title: Effect of NaCl and NaHCO3 on serum ionised calcium and blood gas status during sprinting.
abstract: Sodium bicarbonate is often administered to horses before racing in an attempt to delay fatigue and improve performance. We examined the effect of acid-base status on serum ionised calcium concentration (iCa) during high intensity exercise in 8 Standardbred mares. In a randomised, blinded, cross-over study, mares were administered each of 3 treatments, NaCl (0.7 g/kg bwt), NaHCO3 (1 g/kg bwt) in 3 l water, or 3 l of water only, 4 h before performing a standardised exercise test to fatigue on a treadmill. Mixed venous blood samples were collected as the horses ran for 5 min at 3 m/s, to fatigue at a predetermined speed (approximately 113% VO2max) and for 5 min at 3 m/s. There was no effect of treatment on time to fatigue (P = 0.744). NaHCO3 attenuated (P<0.05) the exercise-induced decrease in venous pH (mean +/- s.e. 6.97, 6.95 and 7.06 +/- 0.02 at end of sprint for water, NaCl and NaHCO3, respectively). Both serum total calcium concentration (tCa) and iCa increased (P<0.05) with running. NaHCO3 decreased iCa (P<0.05) compared to water; iCa of 1.58 and 1.44 +/- 0.04 mmol/l before exercise and 1.69 and 1.49 +/- 0.05 end sprint, for water and NaHCO3 treatments, respectively. These results demonstrate an effect of NaHCO3 on iCa during exercise. Further study is necessary to determine the effect of alterations in iCa on exercise performance.
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus 43210, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Beard
authors.forename: K W
authors.initials: KW
authors.lastname: Hinchcliff
mesh_terms: D000136:Acid-Base Equilibrium / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D001784:Blood Gas Analysis / Q000662:veterinary; D002118:Calcium / Q000097:blood*; D018592:Cross-Over Studies; D004573:Electrolytes / Q000097:blood; D005260:Female; D006736:Horses / Q000097:blood / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D010805:Physical Conditioning, Animal / Q000502:physiology*; D005082:Physical Exertion / Q000502:physiology; D012420:Running / Q000502:physiology; D017693:Sodium Bicarbonate / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D004573:Electrolytes; D012965:Sodium Chloride; D017693:Sodium Bicarbonate; D002118:Calcium