title: 23-Hydroxybetulinic acid-mediated apoptosis is accompanied by decreases in bcl-2 expression and telomerase activity in HL-60 Cells.
abstract: 23-Hydroxybetulinic acid, a derivative of betulinic acid, was investigated for its apoptotic effect and the associated telomerase activity in human leukemia HL-60 cells. Apoptosis and bcl-2 were determined by flow cytometry analysis. A PCR-based telomeric repeat amplification protocol assay was used to detect telomerase activity. Results showed that 23-hydroxybetulinic acid induced growth arrest and apoptotic cell death in HL-60 cells. The apoptotic events were associated with concurrent down-regulation of bcl-2 and the telomerase activity. Our data suggest that 23-hydroxybetulinic acid may be a potential cytotoxic agent for treatment of cancer.
authors.affiliation: Department of Pharmacology, China Pharmaceutical University, 210009, Nanjing, China
authors.forename: Zhao-Ning
authors.initials: ZN
authors.lastname: Ji
authors.forename: Wen-Cai
authors.initials: WC
authors.lastname: Ye
authors.forename: Guo-Ging
authors.initials: GG
authors.lastname: Liu
authors.forename: W L Wendy
authors.initials: WL
authors.lastname: Hsiao
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000633:toxicity*; D017209:Apoptosis; D002455:Cell Division / Q000187:drug effects; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D018922:HL-60 Cells; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000276:immunology / Q000378:metabolism*; D019098:Telomerase / Q000378:metabolism*; D014315:Triterpenes / Q000737:chemistry / Q000633:toxicity*
chemical_list: C468651:23-hydroxybetulinic acid; D000972:Antineoplastic Agents, Phytogenic; D019253:Proto-Oncogene Proteins c-bcl-2; D014315:Triterpenes; D019098:Telomerase