title: Mannose-specific lectin activity of parasporal proteins from a lepidoptera-specific Bacillus thuringiensis strain.
abstract: Lectin activity, agglutinating sheep erythrocytes, was associated with parasporal inclusion proteins from a Lepidoptera-specific isolate of Bacillus thuringiensis serovar galleriae (H5ab). The activity was generated when parasporal inclusions were solubilized in an alkaline condition. Proteolytic processing was not required for generation of the lectin activity; the activity level was not affected by the presence/absence of the three proteases (trypsin, chymotrypsin, and proteinase K). SDS-PAGE analysis revealed that (1) alkali-solubilized parasporal inclusion proteins consisted of two major components of 130 kDa and 65 kDa, and (2) proteinase K treatment of alkali-solubilized proteins yielded a single major protein of 60 kDa. Lectin activity of our isolate was strongly inhibited by preincubation with D-mannose, but not with the six other monosaccharides: D-galactose, D-glucose, L-fucose, N-acetyl- D-glucosamine, N-acetyl- D-galactosamine, and N-acetylneuraminic acid. In contrast, D-mannose did not inhibit the in vivo larvicidal activity of the proteins against the silkworm, Bombyx mori.
authors.affiliation: Bioresources and Management Lab, Graduate School of Agriculture, Kyushu University, Fukuoka 812-8581, Japan. wasano@fitc.pref.fukuoka.jp
authors.forename: Naoya
authors.initials: N
authors.lastname: Wasano
authors.forename: Akira
authors.initials: A
authors.lastname: Ohgushi
authors.forename: Michio
authors.initials: M
authors.lastname: Ohba
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics / Q000502:physiology; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D037102:Lectins / Q000302:isolation & purification / Q000494:pharmacology*; D007915:Lepidoptera / Q000382:microbiology; D008358:Mannose / Q000378:metabolism*; D013171:Spores, Bacterial / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D037102:Lectins; D008358:Mannose