title: [Establishment and characterization of human extrahepatic growing hepatocellular carcinoma cell line EGHC-9901].
abstract: OBJECTIVE
  To establish a new extrahepatic growing hepatocellular carcinoma cell line.
  
  
  METHODS
  A specimen from extrahepatic growing hepatocellular carcinoma was cultured in vitro. Cancer cells were studied morphologically and subjected to karyotype analysis, DNA analysis, and tumor formation evaluation.
  
  
  RESULTS
  Morphological observation and functional analysis showed that their features were similar to those of HCC. Chromosomes with a variation of 76 approximately 104 were able to secret AFP in vitro and to form bile canaliculi with microvilli.
  
  
  CONCLUSIONS
  EGHC-9901 cell line has characteristics of the extrahepatic growing hepatocellular carcinoma.
authors.affiliation: Department of General Surgery, Qilu Hospital, Shandong University, Ji'nan 250012, China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Wu
authors.forename: Zhanmin
authors.initials: Z
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Yingmao
authors.initials: Y
authors.lastname: Gao
authors.forename: Zhaoting
authors.initials: Z
authors.lastname: Li
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Liu
mesh_terms: D000328:Adult; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology*; D002869:Chromosome Aberrations; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D000509:alpha-Fetoproteins