title: IgE-binding epitopes of the American cockroach Per a 1 allergen.
abstract: Cockroach is one of the major indoor allergens for IgE-mediated allergic respiratory illnesses throughout the world. The American cockroach (Periplaneta americana) Per a 1 allergen is antigenically cross-reactive with the German cockroach (Blattella germanica) Bla g 1 allergen. The aim of this study was to identify linear B cell epitopes of Per a 1 that are recognized by human IgE. Per a 1 deletion mutants were generated from the recombinant Per a 1.0104 allergen (274 amino acid residues), and antigenicities were assessed by immunoblotting, enzyme-linked immunosorbent assay (ELISA), and binding inhibition. Human atopic sera were not able to recognize deletion mutants consisting of amino acids 1-77, 86-205, and 200-266. However, human sera did recognize the N-terminal mutant containing amino acids 1-87 and the C-terminal mutant containing amino acids 200-274, demonstrating positive IgE binding that was heterogeneously distributed among the different sera tested. Amino acid residues 78-85 and 267-274, containing internal repeats, were shown to be required for IgE binding to the Per a 1.0104 protein. Two peptides corresponding to these IgE-binding amino acid sequences were synthesized. Peptide 78-85 showed a positive IgE interaction with 80% of the sera, while peptide 267-274 was capable of IgE binding to all of the sera tested. Moreover, preincubation of atopic sera with IgE-positive recombinants and peptides resulted in marked inhibition of the IgE binding to purified Per a 1.0104 allergen. Amino acid sequences 78LIRALFGL85 and 267IRSWFGLP1274 of the American cockroach Per a 1.0104 allergen were involved in IgE binding. These findings will advance the understanding of the specific reactivity of the epitopes of cockroach allergens, thereby contributing to the development of specific immunotherapies for clinical use.
authors.affiliation: Department of Education and Research, Taichung Veterans General Hospital, 160 Chung Kang Road, Section 3, Taichung 407, Taiwan. w051441@vghtc.vghtc.gov.tw
authors.forename: Chii-Huei
authors.initials: CH
authors.lastname: Wu
authors.forename: Mey-Fann
authors.initials: MF
authors.lastname: Lee
authors.forename: Jyh-Shyne
authors.initials: JS
authors.lastname: Yang
authors.forename: Ching-Yuan
authors.initials: CY
authors.lastname: Tseng
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000276:immunology*; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D018985:Epitopes, B-Lymphocyte; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007167:Immunotherapy; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D018985:Epitopes, B-Lymphocyte; D011994:Recombinant Proteins; C091463:allergen Per a I; D007073:Immunoglobulin E