[In vitro study on hormonal regulation of human endometrial stroma cell decidualization].
OBJECTIVE
To investigate the roles of luteinizing hormone releasing-hormone (LHRH), follicle stimulating hormone (FSH), human chorionic gonadotropin (hCG) and ovary steroids in regulation of human endometrial stroma cell (ESC) decidualization.


METHODS
Stroma cells derived from human endometrium during the proliferative phase were cultured in vitro and treated with physiological doses of estradiol (E(2)), testosterone (T), progesterone (P) or LHRH, FSH and hCG. Their morphologic changes and prolactin (PRL) production in the media were examined and compared.


RESULTS
Addition of E(2) or T or P stimulated ESC proliferation, resulting in increase of the saturation density. The fibroblast- morphologic changes to polygonal shape and began to express PRL simultaneously after treatment of P or T. P in presence of E(2) or T significantly enhanced PRL production (P < 0.01), suggesting a synergistic action between them in stimulating ESC decidualization. LHRH, FSH or hCG also induced morphologic changes and PRL production by ESC in the presence of E(2) + P. Among them the impact of hCG is most remarkable, especially in this culture system.


CONCLUSIONS
E(2), T and P play an important role in proliferation and differentiation of the ESC. LHRH, FSH, hCG also directly exert an effect on ESC decidualization.
Su
Aiqin
A
Department of Obstetrics and Gynecology, Tianjin First Central Hospital, Tianjin 300192, China.
Mi
Ruoran
R
Zhang
Shuangge
S
Tu
Chikun
C
Zhang
Yuhua
Y
D000328:Adult; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003656:Decidua / Q000502:physiology*; D004717:Endometrium / Q000166:cytology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D006801:Humans; D011388:Prolactin / Q000378:metabolism; D017154:Stromal Cells / Q000187:drug effects / Q000502:physiology
D006063:Chorionic Gonadotropin; D012739:Gonadal Steroid Hormones; D007987:Gonadotropin-Releasing Hormone; D011388:Prolactin; D005640:Follicle Stimulating Hormone