The effects of the total saponin of Dipsacus asperoides on the damage of cultured neurons induced by beta-amyloid protein 25-35.
According to the pathogenesis of Alzheimer's disease (AD), beta-amyloid protein (A beta) was directly toxic to neurons, leading to neurodegeneration. Total saponin of Dipsacus asperoides (tSDA) is one of the main ingredients of Dipsacus asperoide, a traditional Chinese medicine. To explore the effects of tSDA on neuronal damage induced by A beta in vitro, biochemical analysis combining primary cultured neurons were adopted. Neurons were treated with 35 mmol/L A beta for 24 h, and tSDA at concentrations of 1-300 mg/L were added to A beta-treated cultures 24 h in advance, A beta for 24 h, the survival rate of neurons decreased closely by 50%. Lactate dehydrogenase release and the Malondialdehyde (MDA) level increased substantially. However, if neurons were pretreated with tSDA, the survival rate of neurons was higher than A beta-treated alone. Lactate dehydrogenase release and the MDA level decreased distinctly. Results demonstrated that tSDA possessed a neuroprotective action and that tSDA protected neurons against the toxicity of A beta, most likely by relieving oxidative stress or inhibiting the process of A beta, inducing free radical generation.
Qian
Yi-Hua
YH
Department of Anatomy, School of Medicine of Xi'an Jiaotong University, Xi'an 710061, China. qianyh38@mail.xjtu.edu.cn
Liu
Yong
Y
Hu
Hai-Tao
HT
Ren
Hui-Min
HM
Chen
Xin-Lin
XL
Xu
Jie-Hua
JH
D000544:Alzheimer Disease / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D016923:Cell Death / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D006836:Hydro-Lyases / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008315:Malondialdehyde / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D010446:Peptide Fragments / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D012503:Saponins / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles
D016229:Amyloid beta-Peptides; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D012503:Saponins; D013778:Tetrazolium Salts; D013844:Thiazoles; C074636:amyloid beta-protein (25-35); C085393:dipsacus saponin B; D008315:Malondialdehyde; D009828:Oleanolic Acid; D006836:Hydro-Lyases; C045581:lactate dehydratase; C022616:thiazolyl blue