RNA-peptide binding and the effect of inhibitor and RNA mutation studied by on-line acoustic wave sensor.
Acoustic wave devices of the transverse shear-wave type are becoming increasingly important in the study of biochemical binding events at the solid-liquid interface in real time. The operation of the sensor is based on the principle that perturbations occurring at the solid-liquid interface result in changes in the propagating characteristics of the acoustic wave. The binding of the human immunodeficiency virus-type 1 Tat protein to the transactivation-responsive RNA element has been studied using this sensor. Variable acoustic signals in terms of frequency and motional resistance changes are obtained when surface-immobilized RNA is challenged by different peptide fragments derived from Tat protein. The effect of peptide concentration and mutation in addition to the inhibition of RNA-peptide binding by neomycin has been investigated. The results of this study suggest that acoustic physics offers considerable potential for the screening of small-molecule interactions with nucleic acids.
Tassew
Nardos
N
Department of Chemistry, University of Toronto, ON, Canada.
Thompson
Michael
M
D000161:Acoustic Stimulation; D015696:Gene Products, tat / Q000737:chemistry; D007202:Indicators and Reagents; D009154:Mutation; D009355:Neomycin / Q000737:chemistry; D009862:Online Systems; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D012313:RNA / Q000737:chemistry* / Q000235:genetics*
D015696:Gene Products, tat; D007202:Indicators and Reagents; D010455:Peptides; D012313:RNA; D009355:Neomycin