title: Analysis of N-terminal amino acid sequence of 12 000-protein in gingival crevicular fluid and its clinical significance.
abstract: OBJECTIVE
  To study the essence of N-terminal amino acid sequence of 12 000-protein in gingival crevicular fluid (GCF).
  
  
  METHODS
  GCF samples from patients with RPP and AP were collected. 12 000-protein was separated by SDS-PAGE and transformed to PVDF by electronic transformation. The aim band was cut to be analyzed in 491 Protein Sequencer.
  
  
  RESULTS
  The first ten of N-terminal amino acid sequence of 12 000-protein in GCF was Met, Leu, Thr, Glu, Leu, Glu, Lys, Ala, Leu, Asn. Through checking up in MS-Edman, the sequence was similar to "Ca binding protein, MRP8" which is the light subunit of Calprotectin.
  
  
  CONCLUSIONS
  Calprotectin is a major protein in granulocytes and monocytes, and is related to many inflammatory diseases, maybe served as a effective marker for evaluating the inflammation of periodontium.
authors.affiliation: Department of Stomatology, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Rongji
authors.initials: R
authors.lastname: Wang
authors.forename: Huanxin
authors.initials: H
authors.lastname: Meng
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Chen
authors.forename: Caifang
authors.initials: C
authors.lastname: Cao
mesh_terms: D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D006801:Humans; D039841:Leukocyte L1 Antigen Complex; D010519:Periodontium
chemical_list: D039841:Leukocyte L1 Antigen Complex