Cyclic nucleotide content of tobacco BY-2 cells.
The cyclic nucleotide content of cultured tobacco bright yellow-2 (BY-2) cells was determined, after freeze-killing, perchlorate extraction and sequential chromatography, by radioimmunoassay. The identities of the putative cyclic nucleotides, adenosine 3',5'-cyclic monophosphate (cyclic AMP), guanosine 3',5'-cyclic monophosphate (cyclic GMP) and cytidine 3',5'-cyclic monophosphate (cyclic CMP) were unambiguously confirmed by tandem mass spectrometry. The potential of BY-2 cell cultures as a model system for future investigations of cyclic nucleotide function in higher plants is discussed.
Richards
Helen
H
Biochemistry Group, Wallace Building, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK.
Das
Swadipa
S
Smith
Christopher J
CJ
Pereira
Louisa
L
Geisbrecht
Alan
A
Devitt
Nicola J
NJ
Games
David E
DE
van Geyschem
Jan
J
Gareth Brenton
A
A
Newton
Russell P
RP
D002460:Cell Line; D000242:Cyclic AMP / Q000032:analysis* / Q000302:isolation & purification; D003563:Cyclic CMP / Q000032:analysis* / Q000302:isolation & purification; D006152:Cyclic GMP / Q000032:analysis* / Q000302:isolation & purification; D013058:Mass Spectrometry; D014026:Nicotiana / Q000737:chemistry* / Q000166:cytology*
D003563:Cyclic CMP; D000242:Cyclic AMP; D006152:Cyclic GMP