Increase of separation resolution through field enhancement in microchips.
An enhanced ability to separate charged species from neutral compounds in a microfluidic chip is demonstrated using a chip design with low-resistance electrode channels operating with a multiport pressure/voltage controller. A factor of 2.7 improvement in resolution was obtained from chips made using identical mask designs but different etch depth protocols. Greater separation power allows one to cover a wider dynamic range for compounds with different electrophoretic mobilities.
Kerby
Matthew B
MB
Caliper Technologies Corp., 605 Fairchild Drive, Mountain View, CA 94043, USA.
Chien
Ring-Ling
RL
D001120:Arginine / Q000031:analogs & derivatives / Q000302:isolation & purification; D001896:Boron Compounds / Q000302:isolation & purification; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D008904:Miniaturization
D001896:Boron Compounds; D001120:Arginine