[Enhancement of ionizing radiation on liposome-mediated gene delivery in human rectal cancer HR-8348 cells].
OBJECTIVE
To investigate the effect of ionizing radiation on liposome-mediated gene delivery and find out a way to improve gene transfection.


METHODS
Prior to liposome transfection, HR-8348 cells were irradiated at doses of 0, 2, 4, 8 Gy selected according to the surviving fraction line of HR-8348 cells after different dosage of radiation. After 36 h of liposome transfection, green fluorocytes were counted. The transfection efficiency was figured out and compared with each other.


RESULTS
The transfection efficiency of liposome-mediated gene delivery was 21.32%, 62.17%, 68.00%, 77.78% at the dose of 0, 2, 4, 8 Gy respectively and the clinical dose (2 Gy) was as high as 62.17%. Combined radiation and liposome-mediated gene delivery achieved the approximate transfection efficiency of virus vector.


CONCLUSIONS
Ionizing radiation can improve the transfection efficiency of liposome-mediated gene delivery markedly and it is expected to treat human malignancy with liposome-mediated gene delivery combined with radiation.
Lu
Wenping
W
Department of General Surgery, Beijing Military Area General Hospital, Beijing 100700, China.
Li
Shiyong
S
An
Ping
P
Yu
Bo
B
D004307:Dose-Response Relationship, Radiation; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006801:Humans; D008081:Liposomes; D012004:Rectal Neoplasms / Q000628:therapy*; D014162:Transfection
D008081:Liposomes