title: Lactate transport in red blood cells by monocarboxylate transporters.
abstract: The lactate transport activity of red blood cells (RBC) varies widely among different species; in equine RBC, the activity of the main lactate carrier, H+-monocarboxylate co-transporter (MCT), is distributed bimodally. The influence of lactate transport activity is measurable in vivo; after maximal exercise, the RBC lactate concentration in horses with high (HT) lactate transport activity is higher than in those with low (LT) activity. To study the expression of MCT in HT and LT horses, blood samples were taken from 10 horses at rest and after submaximal exercise. Blood and plasma lactate concentrations, lactate and pyruvate transport activities and the amounts of MCT1, MCT2 and MCT4 were measured. After exercise, RBC lactate concentration was higher in HT (n = 5) than in LT (n = 5) horses. At lactate concentrations of 0.25-30 mmol/l and at a pyruvate concentration of 1 mmol/l, transport activity was higher in HT horses. At a lactate concentration of 0.1 mmol/l, transport was similar. In Western blots, the signals for MCT1 and MCT2 were similar in both groups. The amount of CD147, a chaperone necessary for the activity of MCT1, was lower in LT horses. We suggest that MCT2 transports lactate at low concentrations, while MCT1 is needed at higher concentrations. MCT1 may be less active in LT horses and, therefore, during exercise their capacity to take up lactate is low. Further studies are needed to show whether the differences in lactate influx in RBC affect the function of erythrocytes or the performance capacity of horses.
authors.affiliation: Department of Basic Veterinary Sciences, University of Helsinki, Finland.
authors.forename: N M
authors.initials: NM
authors.lastname: Koho
authors.forename: L K
authors.initials: LK
authors.lastname: Väihkönen
authors.forename: A R
authors.initials: AR
authors.lastname: Pösö
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004912:Erythrocytes / Q000378:metabolism*; D005080:Exercise Test / Q000662:veterinary; D005260:Female; D006736:Horses / Q000097:blood*; D007773:Lactates / Q000097:blood*; D008297:Male; D027501:Monocarboxylic Acid Transporters / Q000502:physiology*; D010805:Physical Conditioning, Animal / Q000502:physiology; D011773:Pyruvates / Q000097:blood
chemical_list: D007773:Lactates; D027501:Monocarboxylic Acid Transporters; D011773:Pyruvates