[Analysis of aromatic hydrocarbon catabolic genes in strains isolated from soil in Patagonia].
Seven strains belonging to genus Pseudomonas were isolated from an enrichment with hydrocarbon mixtures. Tests for enzyme activities showed that five strains used predominantly the catabolic meta-pathway for aromatic hydrocarbon degradation. Furthermore, the xylE gene which encodes a catechol 2,3-dioxygenase was amplified by PCR, and in two strains the nahAc gene, a key enzyme for naphthalene catabolism, was also found. The xylE gene might be a good marker to identify aromatic hydrocarbon degrading bacteria in soils from Patagonia.
Vacca
G S
GS
CEIMA, Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia, Km 4, 9000 Comodoro Rivadavia, Chubut, Argentina. cygvacca@satlink.com
Kiesel
B
B
Wünsche
L
L
Pucci
O H
OH
D001118:Argentina; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase; D004269:DNA, Bacterial / Q000235:genetics; D049308:Dioxygenases; D004790:Enzyme Induction; D005798:Genes, Bacterial; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D009281:Naphthalenes / Q000378:metabolism; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011520:Protocatechuate-3,4-Dioxygenase / Q000235:genetics / Q000378:metabolism; D011549:Pseudomonas / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006841:Hydrocarbons, Aromatic; D009281:Naphthalenes; D012989:Soil Pollutants; C031721:naphthalene; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase; D011520:Protocatechuate-3,4-Dioxygenase; C026247:gentisate 1,2-dioxygenase