title: Pathway of dephosphorylation of myo-inositol hexakisphosphate by phytases of legume seeds.
abstract: Using a combination of high-performance ion chromatography analysis and kinetic studies, the pathway of dephosphorylation of myo-inositol hexakisphosphate by the phytases purified from faba bean and lupine seeds, respectively, was established. The data demonstrate that the legume seed phytases under investigation dephosphorylate myo-inositol hexakisphosphate in a stereospecific way. The phytase from faba bean seeds and the phytase LP2 from lupine seeds degrade phytate by sequential removal of phosphate groups via D-Ins(1,2,3,5,6)P(5), D-Ins(1,2,5,6)P(4), D-Ins(1,2,6)P(3), and D-Ins(1,2)P(2) to finally Ins(2)P, whereas the phytases LP11 and LP12 from lupine seeds generate the final degradation product Ins(2)P via D-Ins(1,2,4,5,6)P(5), D-Ins(1,2,5,6)P(4), D-Ins(1,2,6)P(3), and D-Ins(1,2)P(2).
authors.affiliation: Centre for Molecular Biology, Federal Research Centre for Nutrition, Haid-und-Neu-Strasse 9, D-76131 Karlsruhe, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Greiner
authors.forename: Marie
authors.initials: M
authors.lastname: Larsson Alminger
authors.forename: Nils-Gunnar
authors.initials: NG
authors.lastname: Carlsson
authors.forename: Mercedes
authors.initials: M
authors.lastname: Muzquiz
authors.forename: Carmen
authors.initials: C
authors.lastname: Burbano
authors.forename: Carmen
authors.initials: C
authors.lastname: Cuadrado
authors.forename: Mercedes M
authors.initials: MM
authors.lastname: Pedrosa
authors.forename: Carmen
authors.initials: C
authors.lastname: Goyoaga
mesh_terms: D010832:6-Phytase / Q000378:metabolism*; D007887:Fabaceae / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D010766:Phosphorylation; D010833:Phytic Acid / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology*
chemical_list: D010833:Phytic Acid; D010832:6-Phytase