title: Quantitative analysis of tropane alkaloids in biological materials by gas chromatography-mass spectrometry.
abstract: A simple and rapid method for quantitation of tropane alkaloids in biological materials has been developed using an Extrelut column with gas chromatography-mass spectrometry (GC-MS). Biological materials (serum and urine) were mixed with a borate buffer and then applied to an Extrelut column. The adsorbed tropane alkaloids were eluted with dichloromethane before a GC-MS analysis. Atropine-d(3) was used as an internal standard. The extracted tropane alkaloids were converted to trimethylsilyl derivatives prior to GC analysis, to improve the instability of tropane alkaloids from heating and the property of them for a GC column. The recoveries of the compounds, which had been spiked to biological materials, were more than 80%. The GC separation of the derivatives from endogenous impurities was generally satisfactory with the use of a semi-polar capillary column. Tropane alkaloids showed excellent linearity in the range of 10-5000 ng/ml and the limit of detection was 5.0 ng/ml for biological materials. The present method is simple and more rapid than those previously reported, and was applied to a poisoning case. It is useful for the routine analysis of tropane alkaloids in cases of suspected tropane alkaloids poisoning.
authors.affiliation: Department of Legal Medicine, Division of Medical Intelligence and Informatics, Graduate School of Biomedicel Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan. namera@hiroshima-u.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Namera
authors.forename: Mikio
authors.initials: M
authors.lastname: Yashiki
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Hirose
authors.forename: Seiichi
authors.initials: S
authors.lastname: Yamaji
authors.forename: Tadato
authors.initials: T
authors.lastname: Tani
authors.forename: Tohru
authors.initials: T
authors.lastname: Kojima
mesh_terms: D000368:Aged; D000470:Alkaloids / Q000097:blood* / Q000652:urine*; D002849:Chromatography, Gas; D032302:Datura / Q000737:chemistry; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry; D018514:Plant Structures / Q000737:chemistry; D011041:Poisoning / Q000175:diagnosis; D014326:Tropanes / Q000097:blood* / Q000652:urine*
chemical_list: D000470:Alkaloids; D014326:Tropanes