Parallel flow measurements in microstructures by use of a multifocal 4 x 1 diffractive optical fan-out element.
We have developed a multifocal optical fluorescence correlation spectroscopy system for parallel flow analyses. Multifocal excitation was made possible through a 4 x 1 diffractive optical fan-out element, which produces uniform intensity in all four foci. Autocorrelation flow analyses inside a 20 microm x 20 microm square microchannel, with the 4 x 1 fan-out foci perpendicular to the flow direction, made it possible to monitor different flows in all four foci simultaneously. We were able to perform cross-correlation flow analyses by turning the microstructure, thereby having all four foci parallel to the direction of flow. Transport effects of the diffusion as a function of flow and distance could then also be studied.
Blom
Hans
H
Department of Microelectronics and Information Technology, Royal Institute of Technology, Kista, Sweden. blom@ele.kth.se
Johansson
Mathias
M
Gösch
Michael
M
Sigmundsson
Toni
T
Holm
Johan
J
Hård
Sverker
S
Rigler
Rudolf
R
D005456:Fluorescent Dyes; D008855:Microscopy, Electron, Scanning; D008962:Models, Theoretical; D055095:Optics and Photonics; D012235:Rhodamines; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D012235:Rhodamines; C005358:tetramethylrhodamine