Role of a positive regulator of root hair development, CAPRICE, in Arabidopsis root epidermal cell differentiation.
In Arabidopsis, root hairs are formed only from a set of epidermal cells named trichoblasts or hair-forming cells. Previous studies showed CAPRICE (CPC) promotes differentiation of hair-forming cells by controlling a negative regulator, GLABRA2 (GL2), which is preferentially expressed in hairless cells. Here, we show that CPC is also predominantly expressed in the hairless cells, but not in the neighboring hair-forming cells, and that CPC protein moves to the hair-forming cells and represses the GL2 expression. We also show that the N terminus of bHLH protein interacts with CPC and is responsible for the GL2 expression. We propose a model in which CPC plays a key role in the fate-determination of hair-forming cells.
Wada
Takuji
T
Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-Ku, Yokohama, Kanagawa 230-0045, Japan.
Kurata
Tetsuya
T
Tominaga
Rumi
R
Koshino-Kimura
Yoshihiro
Y
Tachibana
Tatsuhiko
T
Goto
Koji
K
Marks
M David
MD
Shimura
Yoshiro
Y
Okada
Kiyotaka
K
D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D018398:Homeodomain Proteins / Q000378:metabolism; D017403:In Situ Hybridization; D010641:Phenotype; D019441:Plant Epidermis / Q000166:cytology / Q000502:physiology*; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D020598:Proto-Oncogene Proteins c-myb / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
C511988:ATR1 protein, Arabidopsis; D029681:Arabidopsis Proteins; C511989:CPC protein, Arabidopsis; C089991:GL2 protein, Arabidopsis; D018398:Homeodomain Proteins; D010940:Plant Proteins; D020598:Proto-Oncogene Proteins c-myb; D011993:Recombinant Fusion Proteins