title: Substrate selectivity of plant and microbial lysophosphatidic acid acyltransferases.
abstract: Linoleic acid (18:2) is found in a large variety of plant oils but to date there is limited knowledge about the substrate selectivity of acyltransferases required for its incorporation into storage triacylglycerols. We have compared the incorporation of oleoyl (18:1) and linoleoyl (18:2) acyl-CoAs onto lysophosphatidic acid acceptors by sub-cellular fractions prepared from a variety of plant and microbial species. Our assays demonstrated: (1). All lysophosphatidic acid acyltransferase (LPA-AT) enzymes tested incorporated 18:2 acyl groups when presented with an equimolar mix of 18:1 and 18:2 acyl-CoA substrates. The ratio of 18:1 to 18:2 incorporation into phosphatidic acid varied between 0.4 and 1.4, indicating low selectivity between these substrates. (2). The presence of either stearoyl (18:0) or oleoyl (18:1) groups at the sn-1 position of lysophosphatidic acid did not affect the selectivity of incorporation of 18:1 or 18:2 into the sn-2 position of phosphatidic acid. (3). All LPA-AT enzymes tested incorporated the saturated palmitoyl (16:0) acyl group from equimolar mixtures of 16:0- and 18:1-CoA. The ratios of 18:1 to 16:0 incorporation are generally much higher than those of 18:1 to 18:2 incorporation, varying between 2.1 and 8.6. (4). The LPA-AT from oil palm kernel is an exception as 18:1 and 16:0 are utilised at comparable rates. These results show that, in the majority of species examined, there is no correlation between the final sn-2 composition of oil or membrane lipids and the ability of an LPA-AT to use 18:2 as a substrate in in vitro assays.
authors.affiliation: School of Biological and Biomedical Sciences, University of Durham, South Road, DH1 3LE, Durham, UK.
authors.forename: Adrian P
authors.initials: AP
authors.lastname: Brown
authors.forename: Antoni R
authors.initials: AR
authors.lastname: Slabas
authors.forename: Helen
authors.initials: H
authors.lastname: Denton
mesh_terms: D000217:Acyltransferases / Q000032:analysis / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D019684:Magnoliopsida / Q000201:enzymology*; D010938:Plant Oils / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013379:Substrate Specificity; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism
chemical_list: D010938:Plant Oils; D014280:Triglycerides; D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase