Activation of p38 MAPK induces cell cycle arrest via inhibition of Raf/ERK pathway during muscle differentiation.
Cell cycle arrest is essential for initiation of muscle differentiation in myoblasts. Given the previously described essential role for p38 MAPK in myogenesis, we undertook the present study to investigate the role of p38 MAPK in the cell cycle arrest that initiates muscle differentiation. p38 MAPK activity increased during, and was required for, muscle differentiation. Inhibition of p38 MAPK stimulated Raf and ERK activities, and induced cell proliferation in differentiation medium. The concomitant inhibition of p38 MAPK and ERK, however, failed to induce differentiation or proliferation. In conclusion, inhibition of the Raf/ERK pathway and the consequent cell cycle arrest is one of the major functions of p38 MAPK during muscle differentiation.
Lee
Jinhwa
J
Department of Biotechnology, Dongseo University, Pusan, Republic of Korea.
Hong
Feng
F
Kwon
Sijoong
S
Kim
Sam Soo
SS
Kim
Dong Ok
DO
Kang
Heung Sun
HS
Lee
Su Jae
SJ
Ha
Joohun
J
Kim
Sung Soo
SS
D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology*; D002454:Cell Differentiation; D002460:Cell Line; D003470:Culture Media; D004789:Enzyme Activation; D007093:Imidazoles / Q000494:pharmacology; D048669:MAP Kinase Kinase 6; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D009206:Myocardium / Q000166:cytology / Q000201:enzymology; D019908:Proto-Oncogene Proteins c-raf / Q000037:antagonists & inhibitors*; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D014162:Transfection; D048051:p38 Mitogen-Activated Protein Kinases
D003470:Culture Media; D007093:Imidazoles; D011725:Pyridines; D019908:Proto-Oncogene Proteins c-raf; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D048669:MAP Kinase Kinase 6; C093642:SB 203580