NMR studies of drug interaction with membranes and membrane-associated proteins.
This review focuses on the recent developments in the study of drug interactions with biological membranes and membrane-associated proteins using nuclear magnetic resonance (NMR) spectroscopy and other spectroscopic techniques. Emphasis is placed on a class of low-affinity neurological agents as exemplified by volatile general anesthetics and structurally related compounds. The technical aspects are reviewed of how to prepare membrane-mimetic systems and of NMR approaches that are either in current use or opening new prospects. A brief literature survey covers studies ranging from drug distribution in simplified lipid matrix to specific drug interaction with neuronal receptors reconstituted in complicated synthetic membrane systems.
Xu
Yan
Y
Department of Anesthesiology and Critical Care Medicine, University of Pittsburgh School of Medicine, PA 15261, USA. xuy@anes.upmc.edu
Yushmanov
Victor E
VE
Tang
Pei
P
D018681:Anesthetics, General / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects*; D005544:Forecasting; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D017136:Ion Transport / Q000187:drug effects; D008081:Liposomes / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry / Q000187:drug effects*; D008823:Micelles; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D025941:Protein Interaction Mapping
D018681:Anesthetics, General; D008081:Liposomes; D008563:Membrane Lipids; D008565:Membrane Proteins; D008823:Micelles