title: [Environmental Isolates of Sporothrix schenckii in China].
abstract: Restriction fragment length polymorphism (RFLP) in mitochondrial DNA (mtDNA) from 25 environmental isolates of Sporothrix schenckii from northeastern China was investigated. Based on the mtDNA-RELP patterns with Hae III, 6 isolates were confirmed to be S. schenckii, while the other 19 isolates were confirmed to be species distinct from S. schenckii. The mtDNA RFLP patterns of the 19 non-S. schenckii were identical to each other. The non-S. schenckii isolates could not be discriminated from S. schenckii by their macro- or micro-morphological features, and were not pathogenic in guinea pigs. Serological and delayed hypersensitivity cross-reactions were found between S. schenckii and the non-S. schenckii species, suggesting antigenic similarity. These results indicate that RFLP analysis of mtDNA is essential for the identification of environmental isolates of S. schenckii.
authors.affiliation: Department of Dermatology, Kanazawa Medical University, Uchinada, Ishikawa 920-0293, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishizaki
authors.forename: Masako
authors.initials: M
authors.lastname: Kawasaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Mochizuki
authors.forename: Xue-Zhu
authors.initials: XZ
authors.lastname: Jin
authors.forename: Saburo
authors.initials: S
authors.lastname: Kagawa
mesh_terms: D000818:Animals; D002681:China; D004271:DNA, Fungal / Q000032:analysis; D004272:DNA, Mitochondrial / Q000032:analysis; D006168:Guinea Pigs; D017720:Molecular Epidemiology / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D013175:Sporothrix / Q000235:genetics / Q000302:isolation & purification*; D013174:Sporotrichosis / Q000382:microbiology
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial