Integration of a membrane-based desalting step in a microfabricated disposable polymer injector for mass spectrometric protein analysis.
A simple desalting procedure for the coupling of a polymer microchip injector to mass spectrometry is proposed. The overall process is based on the adsorption of proteins on a poly(vinylidene difluoride) (PVDF) membrane, which are then directly eluted in the spraying solution. This microchip-based approach has been successfully applied to small drugs, peptides and proteins originally diluted in phosphate-buffered saline (PBS). Moreover, when eluting the retained proteins in small volumes, a preconcentration is obtained. The combination of single-use, mass-produceable, low-sample-consumption, easy-to-automate, miniaturized polymer injectors with easy-to-handle solution-exchange membranes makes this system particularly amenable to screening applications.
Lion
Niels
N
Electrochemistry Laboratory, Swiss Federal Institute of Technology, CH-1015 Lausanne, Switzerland.
Gobry
Véronique
V
Jensen
Henrik
H
Rossier
Joël S
JS
Girault
Hubert
H
D000804:Angiotensin II / Q000302:isolation & purification; D004209:Disposable Equipment; D006801:Humans; D007328:Insulin / Q000302:isolation & purification; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008567:Membranes, Artificial; D008904:Miniaturization; D009211:Myoglobin / Q000302:isolation & purification; D036103:Nanotechnology; D011433:Propranolol / Q000032:analysis
D007328:Insulin; D008567:Membranes, Artificial; D009211:Myoglobin; D000804:Angiotensin II; D011433:Propranolol