title: Activation of p38 MAPK induces cell cycle arrest via inhibition of Raf/ERK pathway during muscle differentiation.
abstract: Cell cycle arrest is essential for initiation of muscle differentiation in myoblasts. Given the previously described essential role for p38 MAPK in myogenesis, we undertook the present study to investigate the role of p38 MAPK in the cell cycle arrest that initiates muscle differentiation. p38 MAPK activity increased during, and was required for, muscle differentiation. Inhibition of p38 MAPK stimulated Raf and ERK activities, and induced cell proliferation in differentiation medium. The concomitant inhibition of p38 MAPK and ERK, however, failed to induce differentiation or proliferation. In conclusion, inhibition of the Raf/ERK pathway and the consequent cell cycle arrest is one of the major functions of p38 MAPK during muscle differentiation.
authors.affiliation: Department of Biotechnology, Dongseo University, Pusan, Republic of Korea.
authors.forename: Jinhwa
authors.initials: J
authors.lastname: Lee
authors.forename: Feng
authors.initials: F
authors.lastname: Hong
authors.forename: Sijoong
authors.initials: S
authors.lastname: Kwon
authors.forename: Sam Soo
authors.initials: SS
authors.lastname: Kim
authors.forename: Dong Ok
authors.initials: DO
authors.lastname: Kim
authors.forename: Heung Sun
authors.initials: HS
authors.lastname: Kang
authors.forename: Su Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Joohun
authors.initials: J
authors.lastname: Ha
authors.forename: Sung Soo
authors.initials: SS
authors.lastname: Kim
mesh_terms: D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology*; D002454:Cell Differentiation; D002460:Cell Line; D003470:Culture Media; D004789:Enzyme Activation; D007093:Imidazoles / Q000494:pharmacology; D048669:MAP Kinase Kinase 6; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D009206:Myocardium / Q000166:cytology / Q000201:enzymology; D019908:Proto-Oncogene Proteins c-raf / Q000037:antagonists & inhibitors*; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D014162:Transfection; D048051:p38 Mitogen-Activated Protein Kinases
chemical_list: D003470:Culture Media; D007093:Imidazoles; D011725:Pyridines; D019908:Proto-Oncogene Proteins c-raf; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D048669:MAP Kinase Kinase 6; C093642:SB 203580