title: Histopathologic and histochemical characterization of calcified structures in hamsters inoculated with Paracoccidioides brasiliensis.
abstract: In pathogenicity studies of 31 Paracoccidioides brasiliensis isolates preserved using Castellani's method we intraperitoneally inoculated 104 young adult hamsters and found laminated concentric structures and calcified appearance that resembled Schaumann bodies, in 43 of them, especially in animals with apparently good condition. We characterized these structures histologically and histochemically using different stains (PAS, Grocott, haematoxylin-eosin, Von Kossa). The Von Kossa staining revealed calcium in these structures. Similar structures have been described in patients with sarcoidosis and also in hamsters inoculated with P. brasiliensis. We found no correlation between the presence of these calcifications and serum calcium levels.
authors.affiliation: Medical Mycology Section, Institute for Tropical Medicine, Universidad Central de Venezuela, Caracas, Venezuela. somae50@hotmail.com
authors.forename: S M
authors.initials: SM
authors.lastname: Essayag
authors.forename: M E
authors.initials: ME
authors.lastname: Landaeta
authors.forename: C
authors.initials: C
authors.lastname: Hartung
authors.forename: S
authors.initials: S
authors.lastname: Magaldi
authors.forename: L
authors.initials: L
authors.lastname: Spencer
authors.forename: R
authors.initials: R
authors.lastname: Suárez
authors.forename: F
authors.initials: F
authors.lastname: García
authors.forename: E
authors.initials: E
authors.lastname: Pérez
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000032:analysis; D002114:Calcinosis; D006224:Cricetinae; D004195:Disease Models, Animal; D006099:Granuloma / Q000276:immunology / Q000473:pathology; D010228:Paracoccidioides / Q000166:cytology* / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D010229:Paracoccidioidomycosis / Q000382:microbiology* / Q000473:pathology; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000908:Antibodies, Fungal