Simple microdilution test for detection of metallo-beta-lactamase production in Pseudomonas aeruginosa.
A microdilution test measuring imipenem MICs in the presence or absence of a mixture of EDTA plus 1,10-phenanthroline was developed and tested on 190 Pseudomonas aeruginosa isolates, including 18 VIM- and 4 IMP-type metallo-beta-lactamase (MBL) producers. The chelator mixture reduced by fourfold or more the imipenem MICs for MBL producers, while a lower effect or no effect was usually observed with MBL nonproducers.
Migliavacca
Roberta
R
Dipartimento di Scienze Morfologiche, Eidologiche e Cliniche, Sez. di Microbiologia, Università di Pavia, 27100 Pavia, Italy. lpagani@unipv.it
Docquier
Jean-Denis
JD
Mugnaioli
Claudia
C
Amicosante
Gianfranco
G
Daturi
Rossana
R
Lee
Kyungwon
K
Rossolini
Gian Maria
GM
Pagani
Laura
L
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002614:Chelating Agents; D004492:Edetic Acid; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010618:Phenanthrolines; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology*; D001618:beta-Lactamases / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D002614:Chelating Agents; D010618:Phenanthrolines; D015378:Imipenem; D004492:Edetic Acid; D001618:beta-Lactamases; C025205:1,10-phenanthroline