title: Membrane-bound ATPase contributes to hop resistance of Lactobacillus brevis.
abstract: The activity of the membrane-bound H+-ATPase of the beer spoilage bacterium Lactobacillus brevis ABBC45 increased upon adaptation to bacteriostatic hop compounds. The ATPase activity was optimal around pH 5.6 and increased up to fourfold when L. brevis was exposed to 666 microM hop compounds. The extent of activation depended on the concentration of hop compounds and was maximal at the highest concentration tested. The ATPase activity was strongly inhibited by N,N'-dicyclohexylcarbodiimide, a known inhibitor of FoF1-ATPase. Western blots of membrane proteins of L. brevis with antisera raised against the alpha- and beta-subunits of FoF1-ATPase from Enterococcus hirae showed that there was increased expression of the ATPase after hop adaptation. The expression levels, as well as the ATPase activity, decreased to the initial nonadapted levels when the hop-adapted cells were cultured further without hop compounds. These observations strongly indicate that proton pumping by the membrane-bound ATPase contributes considerably to the resistance of L. brevis to hop compounds.
authors.affiliation: Fundamental Research Laboratory, Asahi Breweries, Ltd., Moriya-shi, Ibaraki 302-0106, Japan. kanta.sakamoto@asahibeer.co.jp
authors.forename: Kanta
authors.initials: K
authors.lastname: Sakamoto
authors.forename: H W
authors.initials: HW
authors.lastname: Van Veen
authors.forename: Hiromi
authors.initials: H
authors.lastname: Saito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Wil N
authors.initials: WN
authors.lastname: Konings
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015153:Blotting, Western; D004791:Enzyme Inhibitors / Q000494:pharmacology; D027582:Humulus / Q000737:chemistry*; D007778:Lactobacillus / Q000187:drug effects* / Q000201:enzymology; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D008565:Membrane Proteins; D010936:Plant Extracts; D000251:Adenosine Triphosphatases