Expression of B7 costimulator in eight human malignant hematological cell lines and its significance.
OBJECTIVE
To explore the expression of B7 co-stimulator in human malignant hematological cell lines and its significance.


METHODS
Amplified and purified plasmid DNA was transfected by liposome and detection of B7 gene expression by flow cytometry and RT-PCR.


RESULTS
Eight human malignant hematological cell lines U937, K562, CEM, Daudi, GM, PEER, Jurkat, Raji express high level of HLA molecules, low or undetectable level of B7-1 gene, and high level of B7-2 gene. Expression level of B7-1 gene was enhanced after B7-1 transfection. T cell expressed high level of IL-2 mRNA after B7-1 transfection.


CONCLUSIONS
Multiple human malignant hematological cell lines express low or undetectable level of B7-1 gene, suggesting that B7-1 plays a critical role in tumor immunity.
Jia
Liping
L
The Third Hospital, Beijing University, Beijing 100083, China.
Ke
Xiaoyan
X
Wang
Jing
J
D018122:B7-1 Antigen; D002460:Cell Line; D005434:Flow Cytometry; D006801:Humans; D007376:Interleukin-2; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection; D020298:U937 Cells
D018122:B7-1 Antigen; D007376:Interleukin-2