Expression of prostaglandin I(2) synthase, but not prostaglandin E synthase, changes in myometrium of women at term pregnancy.
Prostaglandins (PGs) act as potent uterotonins at the time of labor. Prostaglandin E synthase (PGES) is responsible for the formation of PGE(2), a uterotonin. PGI(2) is synthesized by the prostaglandin I synthase enzyme (PGIS) and contributes to relaxation in the lower uterine segment. We examined the expression of membrane-bound PGES and PGIS in myometrium from pregnant women during preterm and term labor. Tissues were collected from the lower uterine segment from preterm no labor, preterm labor, term no labor, and term labor patients and used for immunohistochemistry and Western blot analysis using specific antibodies. Immunoreactive (ir-) PGES and PGIS proteins were localized to the cytoplasm of myocytes of the myometrium and vascular smooth muscle cells. Ir-PGES was also detected in vascular endothelial cells. Western blot analyses revealed a predominant protein band of 180 kDa, and a second 16-kDa band for ir-PGES and 56-kDa band for ir-PGIS. There was no significant change in ir-PGES protein (180 or 16 kDa) or mRNA levels with preterm or term labor or gestational age. There was a significant decrease in PGIS mRNA and protein with advancing gestational age. We conclude that the gestational age decrease in the inhibitory PGIS is consistent with lessening of its influence in myometrium at the time of labor. The lack of change in PGES indicates that alterations at other points along the pathway of arachidonic acid metabolism may be of greater importance in affecting local changes in PGE(2).
Giannoulias
D
D
Canadian Institutes of Health Research in Human Development, Department of Physiology, University of Toronto, 1 King's College Circle, Medical Sciences Building Room 3344, Toronto, Ontario, Canada M5S 1A8. diana.giannoulias@utoronto.ca
Alfaidy
N
N
Holloway
A C
AC
Gibb
W
W
Sun
M
M
Lye
S J
SJ
Challis
J R G
JR
D000199:Actins / Q000235:genetics; D015153:Blotting, Western; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D003593:Cytoplasm / Q000201:enzymology; D004730:Endothelium, Vascular / Q000201:enzymology; D005260:Female; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D015870:Gene Expression; D005865:Gestational Age; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D019746:Intramolecular Oxidoreductases / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D007743:Labor, Obstetric / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000201:enzymology; D009215:Myometrium / Q000098:blood supply / Q000378:metabolism*; D011247:Pregnancy; D000071876:Prostaglandin-E Synthases; D012333:RNA, Messenger / Q000032:analysis
D000199:Actins; D005456:Fluorescent Dyes; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; C022461:prostacyclin synthetase