Effect of dietary lipid on response to exercise: relationship to metabolic adaptation.
The aim of the present study was to relate changes in muscle oxidative capacity and free fatty acid flux in response to oil supplementation to fuel utilisation during subsequent exercise of varying intensities. Following 10 weeks of oil supplementation there was an increased capacity for fat utilisation during low and moderate intensity exercise as indicated by a lower respiratory exchange ratio (RER) (P<0.05). We suggest that this was contributed to by a parallel increase in the oxidative capacity of muscle as indicated by a significant increase in the activity of muscle citrate synthase (CS) (P<0.05) and trend towards an increase in beta-Hydroxy acyl CoA dehydrogenase (beta-HAD), (P>0.05). In addition, low and moderate intensity exercise was associated with an exercise-induced increase in plasma free fatty acids (FFA) and there was an increased facility for uptake of FFA by working muscle from circulating triglycerides, as suggested by an increase in TL activity (P<0.01). The response to oil supplementation varied between individual horses and the magnitude of response, during the low intensity exercise test, in terms of difference in RER was correlated to the increase in CS activity (r2 = 0.95, P<0.05) following oil supplementation. There was no similar significant correlation with respect to FFA, TL or beta-HAD activity (P>0.05). The hypothesis in this study was that the metabolic adaptation to oil supplementation, in terms of exercise response, was related to individual increases in the activities of CS, beta-HAD or TL. However, the relationship between these parameters was unequivocal and requires further investigation, ideally with a larger group of horses.
Dunnett
C E
CE
Centre for Equine Studies, Animal Health Trust, Newmarket, Suffolk, UK.
Marlin
D J
DJ
Harris
R C
RC
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D000222:Adaptation, Physiological; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage* / Q000378:metabolism; D005080:Exercise Test / Q000662:veterinary; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005684:Gait / Q000502:physiology; D006736:Horses / Q000378:metabolism* / Q000502:physiology; D008049:Lipase / Q000378:metabolism; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism*; D010101:Oxygen Consumption; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011659:Pulmonary Gas Exchange / Q000502:physiology*; D011897:Random Allocation; D013024:Soybean Oil; D014280:Triglycerides / Q000097:blood
D004041:Dietary Fats; D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D013024:Soybean Oil; D015094:3-Hydroxyacyl CoA Dehydrogenases; D002950:Citrate (si)-Synthase; D008049:Lipase