Significant differences between procyclic and bloodstream forms of Trypanosoma brucei in the maintenance of their plasma membrane potential.
The plasma membrane potential (deltapsi) of procyclic and bloodstream trypomastigotes of Trypanosoma brucei was studied using the potentiometric fluorescent dye bisoxonol. Our results suggest that a proton pump plays a significant role in the regulation of deltapsi in procyclic and bloodstream forms, as evidenced by depolarization of the plasma membrane by H(+)-ATPase inhibitors (e.g. dicyclohexylcarbo-diimide, N-ethylmaleimide, diethylstilbestrol, and bafilomycin A1). In bloodstream stages the plasma membrane was significantly depolarized by ouabain only when the cells were incubated in sodium-rich buffers indicating that a sodium pump was being inhibited. In contrast, ouabain had no effect on the deltapsi of the procyclic stages in a sodium-rich buffer. However, it induced an additional significant depolarization in these stages when their plasma membrane was already partially depolarized by the H(+)-ATPase inhibitor dicyclohexylcarbo-diimide, indicating the presence of an ouabain-sensitive sodium pump whose activity is masked by the H(+)-ATPase. Unlike procyclic forms, the deltapsi of bloodstream-stage trypomastigotes was markedly sensitive to extracellular Na+ and K+ concentrations. Thus, there are significant differences between procyclic and bloodstream forms in the maintenance of the deltapsi and in their permeability to cations.
Van der Heyden
Nicole
N
Department of Pathobiology, University of Illinois at Urbana-Champaign, 61802, USA.
Docampo
Roberto
R
D000818:Animals; D001769:Blood / Q000469:parasitology; D002462:Cell Membrane / Q000502:physiology*; D002463:Cell Membrane Permeability; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials / Q000502:physiology*; D051379:Mice; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase; D013696:Temperature; D014346:Trypanosoma brucei brucei / Q000254:growth & development* / Q000502:physiology
D006180:Proton-Translocating ATPases; D000254:Sodium-Potassium-Exchanging ATPase