Microbial transformation of rubijervine.
Preparative-scale fermentation of rubijervine (1), the known 22,26-epiminocholestane Veratrum alkaloid, with Cunninghamella echinulata ATCC 9244 has resulted in the isolation of the new metabolites 7alpha-hydroxyrubijervine (2) and solanid-5-ene-3beta,12alpha-diol-1-one (3). Structure elucidation of these metabolites was based primarily on 1D- and 2D-NMR analyses. The microbe C. echinulata ATCC 9244 was able to metabolize rings A and B of rubijervine but failed to metabolize rings C, D or its N-containing side chain, a finding which is analogous to the results of previous fermentation studies of steroidal alkaloids.
El Sayed
Khalid A
KA
Pharmacognosy Department, Faculty of Pharmacy, Mansoura University, Egypt. elsayed@ulm.edu
Dunbar
D Chuck
DC
D000959:Antihypertensive Agents / Q000737:chemistry / Q000493:pharmacokinetics; D001711:Biotransformation / Q000502:physiology; D020093:Cunninghamella / Q000378:metabolism*; D005285:Fermentation; D014704:Veratrum Alkaloids / Q000737:chemistry* / Q000493:pharmacokinetics*
D000959:Antihypertensive Agents; D014704:Veratrum Alkaloids