Zinc concentration effect at the organismal, cellular and subcellular levels in the eastern oyster.
The purpose of this research was to demonstrate a concentration effect of zinc exposure at organismal, cellular and sub-cellular levels in the eastern oyster and to find associated protein expression signatures (PES) for each concentration of zinc. Oysters were exposed to six concentrations of zinc for 48 h in a controlled environment. At the organismal level, fecal material was observed as a measure of physiological health during metal exposures. At the cellular level, lysosomal destabilization was measured using hemolymph. This cellular response was significant only at the highest concentration, when the fecal index was lowest. Protein responses were monitored in the oyster following exposure to zinc. Gill tissue was excised and homogenized, and then analyzed using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and digital image analysis. Protein expression signatures were found to be specific to each concentration. The protein responses were linked to the other biological parameters measured, each of which followed a concentration gradient of zinc.
Meiller
Jessica C
JC
Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore 21250, USA.
Bradley
Brian P
BP
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D004781:Environmental Exposure; D005243:Feces / Q000737:chemistry; D005880:Gills / Q000502:physiology; D006304:Health Status; D006458:Hemolymph; D008247:Lysosomes / Q000473:pathology*; D010125:Ostreidae / Q000502:physiology*; D014176:Protein Biosynthesis; D014018:Tissue Distribution; D014873:Water Pollutants / Q000009:adverse effects* / Q000493:pharmacokinetics; D015032:Zinc / Q000009:adverse effects* / Q000493:pharmacokinetics
D014873:Water Pollutants; D015032:Zinc