In situ conversion of protochlorophyllide b to protochlorophyllide a in barley. Evidence for a novel role of 7-formyl reductase in the prolamellar body of etioplasts.
We recently put forth a model of a protochlorophyllide (Pchlide) light-harvesting complex operative during angiosperm seedling de-etiolation (Reinbothe, C., Lebedev, N., and Reinbothe, S. (1999) Nature 397, 80-84). This model, which was based on in vitro reconstitution experiments with zinc analogs of Pchlide a and Pchlide b and the two NADPH:protochlorophyllide oxidoreductases (PORs), PORA and PORB, of barley, predicted a 5-fold excess of Pchlide b, relative to Pchlide a, in the prolamellar body of etioplasts. Recent work (Scheumann, V., Klement, H., Helfrich, M., Oster, U., Schoch, S., and Rüdiger, W. (1999) FEBS Lett. 445, 445-448), however, contradicted this model and reported that Pchlide b would not be present in etiolated plants. Here we demonstrate that Pchlide b is an abundant pigment in barley etioplasts but is rather metabolically unstable. It is rapidly converted to Pchlide a by virtue of 7-formyl reductase activity, an enzyme that had previously been implicated in the chlorophyll (Chl) b to Chl a reaction cycle. Our findings suggest that etiolated plants make use of 7-formyl reductase to fine tune the levels of Pchlide b and Pchlide a and thereby may regulate the steady-state level of light-harvesting POR-Pchlide complex.
Reinbothe
Steffen
S
Université Joseph Fourier et CNRS, UMR 5575, BP53, CERMO, F-38041 Grenoble cedex 9, France.
Pollmann
Stephan
S
Reinbothe
Christiane
C
D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D002851:Chromatography, High Pressure Liquid; D001467:Hordeum / Q000378:metabolism*; D010088:Oxidoreductases / Q000502:physiology; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011521:Protochlorophyllide / Q000378:metabolism*
D002734:Chlorophyll; D011521:Protochlorophyllide; C037184:chlorophyll b; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C023886:protochlorophyllide reductase; D000077194:Chlorophyll A