title: Taurine modulates induction of cytochrome P450 3A4 mRNA by rifampicin in the HepG2 cell line.
abstract: Taurine is not only present in foods, tonics and nutrient drinks but is also used as a medicinal agent mainly for treatment of chronic heart failure and liver disease. However, little is known about its influence on drug-metabolizing enzymes, especially cytochrome P450 (CYP), in human. We examined whether taurine could affect the expression of CYP3A4 mRNA in the presence or absence of rifampicin (RFP), which is a potent inducer of CYPs, with HepG2 cells. Taurine enhanced twice the induction of CYP3A4 mRNA by RFP, but did not affect the expression by itself. This effect was both concentration- and time-dependent. On the other hand, taurine did not affect the induction by phenobarbital. Taurine did not increase intracellular uptake of RFP. Therefore, we conclude that taurine is an enhancer for the induction of CYP3A4 by RFP.
authors.affiliation: Clinical Evaluation of Medicines and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Hideyasu
authors.initials: H
authors.lastname: Matsuda
authors.forename: Kayoko
authors.initials: K
authors.lastname: Kinoshita
authors.forename: Akihiko
authors.initials: A
authors.lastname: Sumida
authors.forename: Koichi
authors.initials: K
authors.lastname: Takahashi
authors.forename: Shuichi
authors.initials: S
authors.lastname: Fukuen
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Fukuda
authors.forename: Kyoko
authors.initials: K
authors.lastname: Takahashi
authors.forename: Isamu
authors.initials: I
authors.lastname: Yamamoto
authors.forename: Junichi
authors.initials: J
authors.lastname: Azuma
mesh_terms: D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis* / Q000187:drug effects; D002460:Cell Line; D051544:Cytochrome P-450 CYP3A; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004790:Enzyme Induction / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D010089:Oxidoreductases, N-Demethylating / Q000096:biosynthesis* / Q000187:drug effects; D010634:Phenobarbital / Q000494:pharmacology; D012293:Rifampin / Q000494:pharmacology*; D013654:Taurine / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors; D015091:beta-Alanine / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D015091:beta-Alanine; D013654:Taurine; D001189:Aryl Hydrocarbon Hydroxylases; D051544:Cytochrome P-450 CYP3A; D010089:Oxidoreductases, N-Demethylating; D012293:Rifampin; D010634:Phenobarbital