title: Cloning and sequence analysis of cDNA and genomic DNA of human thrombopoietin gene.
abstract: OBJECTIVE: To study the role of thrombopoietin (TPO) gene 5' untranslated region (UTR) and the intron in TPO expression regulation. METHODS: With the mRNA derived from Chinese human fetal liver as the template, full-length TPO cDNA (approximately 1.1 kb) and TPO genomic DNA(6.2 kb) along with all the introns of TPO gene were isolated by PCR and long-distance PCR techniques from Chinese fetal liver tissue. RESULT: Sequencing analysis indicated that all the coding sequences and the exon/intron splice sites were consistent with the results previously reported by Sohma et al except for only a DNA and all the introns in the intron of TPO gene. CONCLUSION: We have successfully cloned full-length TPO cDNA, TPO genomic DNA and all the introns of TPO gene from Chinese fetal liver tissue.
authors.affiliation: Institute of Molecular Immunology, First Military Medical University, Guangzhou 510515, China.
authors.forename: Yun-Shan
authors.initials: YS
authors.lastname: Ning
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Zhou
authors.forename: Ming-Qian
authors.initials: MQ
authors.lastname: Zhou
authors.forename: Ze-Hong
authors.initials: ZH
authors.lastname: Chen
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Fang
authors.forename: Ning
authors.initials: N
authors.lastname: Fu
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Wang