Enhanced immunocytochemical expression of antioxidant enzymes in rat submandibular gland after normobaric oxygenation.
In order to clarify the role of antioxidant enzymes in the male rat submandibular gland against short-term normobaric oxygenation, we performed immunocytochemical staining of manganese-containing superoxide dismutase (Mn-SOD), copper- and zinc-containing SOD (Cu/Zn-SOD), catalase (CAT), glutathione peroxidase, and glutathione S-transferases (GST alpha, GST mu, and GST pi) between days 1 and 7 after normobaric oxygenation. Ultrastructural alterations and immunoreactivities for malondialdehyde (MDA), a lipid peroxidation-related molecule, of the acinar and ductal cells after the oxygenation were also investigated. Immunoreactivity for MDA was exhibited in the acinar cells throughout the experiment. On the other hand, immunoreactivity for the SODs, CAT, and GSTs was not altered, when compared to that of controls, but was significantly elevated in the granular, striated, and excretory ductal cells. Since an increase of lipid peroxidation as indicated by enhanced immunoreactivity for MDA was detected in the acinar and intercalated ductal cells, the results indicate that the enhanced antioxidant enzymes in the granular, striated, and excretory ductal cells play a crucial role in the self-defense system of the male rat submandibular gland against normobaric oxygenation.
Morita
Miyako
M
Department of Oral and Maxillofacial Surgery, University of Occupational and Environmental Health, School of Medicine, Yahata Nishi-ku, Kitakyushu, Japan. v-miya@med.ueoh-u.ac.jp
Kudo
Hideaki
H
Doi
Yoshiaki
Y
Hirano
Takeshi
T
Ikemura
Kunio
K
Fujimoto
Sunao
S
D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D001273:Atmosphere Exposure Chambers; D002374:Catalase / Q000032:analysis / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005979:Glutathione Peroxidase / Q000032:analysis / Q000378:metabolism; D005982:Glutathione Transferase / Q000032:analysis / Q000378:metabolism; D007124:Immunoenzyme Techniques; D019570:Inhalation Exposure; D008297:Male; D010100:Oxygen / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D013363:Submandibular Gland / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism
D000975:Antioxidants; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D010100:Oxygen