Flow cytometric DNA analysis on fine needle aspiration biopsies of liver lesions.
Flow cytometric DNA analysis on fine needle aspiration biopsies of liver lesions The DNA cell content of 39 fine needle aspiration biopsies (FNAs) from five benign liver lesions, nine hepatocellular carcinomas (HCCs), and 25 metastatic tumours was analysed in a prospective fashion by flow cytometry (FCM). All benign lesions were diploid. Aneuploidy was found in five (55.6%) HCCs and in nine (36%) metastatic tumours. DNA index (DI) differences were not significant. The S-phase fraction (SPF) was higher in the malignant tumours, both combined (P < 0.02) and separated primary and metastatic (P < 0.05). We could not demonstrate an association between diploidy and percentage of benign hepatocytes in the smears of malignant tumours. The serum alpha-fetoprotein (AFP) level did not correlate with ploidy, DI, or SPF in the HCCs. In conclusion, ploidy and DI do not discriminate between benign and malignant liver lesions, but the SPF is higher in malignant tumours. DNA analysis does not help to distinguish primary from metastatic liver tumours. The presence of benign hepatocytes in samples from malignant tumours does not seem to influence the analysis of ploidy by FCM.
Granados
Rosario
R
Department of Pathology, Hospital Universitario de Getafe, Getafe, Madrid, Spain. rgranado@teleline.es
García-Vela
Jose-Antonio
JA
Fenández-Segoviano
Pilar
P
Aramburu
José-Antonio
JA
Martín
Isaac
I
Murillo
Nieves
N
Camarmo
Encarnación
E
De La Cal
Miguel Angel
MA
D000782:Aneuploidy; D001707:Biopsy, Needle; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000235:genetics* / Q000556:secondary; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology; D011446:Prospective Studies; D016196:S Phase / Q000235:genetics; D000509:alpha-Fetoproteins / Q000032:analysis
D004273:DNA, Neoplasm; D000509:alpha-Fetoproteins