Multiple telomeric aberrations in a telomerase-positive leukemia patient.
Bone marrow samples from a pancytopenia/leukemia patient were routinely analyzed at first and second admission. At the first presentation, the karyotype was normal, whereas 17 months later several chromosome aberrations were recognized including presumed additions to the short arms of chromosomes 1 and 16 in all cells, and numerous other aberrations in subpopulations of cells. From the predominance of aberrations at chromosome ends, we suspected insufficient telomere maintenance as an underlying mechanism behind the karyotype changes, in particular as an interstitial deletion in the region harboring the gene for the RNA component (hTERC) of the telomerase enzyme was also noticed; however, while molecular cytogenetic investigation confirmed the terminal aberrations, we found the malignant cells positive for telomerase activity and the presence of an hTERC gene on both chromosomes 3. A presumed chromosome 1 addition turned out to reflect an amplification of a tandemly repeated sequence element. Labeling of multiple tandem repeat sequences in situ by a novel multicolor primed in situ hybridization showed no evidence of instability of other repeated DNA elements.
Serakinci
Nedime
N
Cancer Cytogenetics Laboratory, Department of Hematology, Aarhus Amtssygehus, DK-8000, Aarhus C., Denmark.
Østergaard
Mette
M
Larsen
Herdis
H
Madsen
Bente
B
Pedersen
Bent
B
Koch
Jørn
J
D000328:Adult; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D007938:Leukemia / Q000201:enzymology* / Q000235:genetics*; D008297:Male; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000235:genetics*
D019098:Telomerase