Analysis of N-terminal amino acid sequence of 12 000-protein in gingival crevicular fluid and its clinical significance.
OBJECTIVE
To study the essence of N-terminal amino acid sequence of 12 000-protein in gingival crevicular fluid (GCF).


METHODS
GCF samples from patients with RPP and AP were collected. 12 000-protein was separated by SDS-PAGE and transformed to PVDF by electronic transformation. The aim band was cut to be analyzed in 491 Protein Sequencer.


RESULTS
The first ten of N-terminal amino acid sequence of 12 000-protein in GCF was Met, Leu, Thr, Glu, Leu, Glu, Lys, Ala, Leu, Asn. Through checking up in MS-Edman, the sequence was similar to "Ca binding protein, MRP8" which is the light subunit of Calprotectin.


CONCLUSIONS
Calprotectin is a major protein in granulocytes and monocytes, and is related to many inflammatory diseases, maybe served as a effective marker for evaluating the inflammation of periodontium.
Wang
Rongji
R
Department of Stomatology, Peking University People's Hospital, Beijing 100044, China.
Meng
Huanxin
H
Chen
Zhibin
Z
Cao
Caifang
C
D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D006801:Humans; D039841:Leukocyte L1 Antigen Complex; D010519:Periodontium
D039841:Leukocyte L1 Antigen Complex