The systemin precursor gene regulates both defensive and developmental genes in Solanum tuberosum.
Transformation of Solanum tuberosum, cv. Desiree, with the tomato prosystemin gene, regulated by the 35S cauliflower mosaic virus promoter, resulted in constitutive increase in defensive proteins in potato leaves, similar to its effects in tomato plants, but also resulted in a dramatic increase in storage protein levels in potato tubers. Tubers from selected transformed lines contained 4- to 5-fold increases in proteinase inhibitor I and II proteins, >50% more soluble and dry weight protein, and >50% more total nitrogen and total free amino acids than found in wild-type tubers. These results suggest that the prosystemin gene plays a dual role in potato plants in regulating proteinase inhibitor synthesis in leaves in response to wounding and in regulating storage protein synthesis in potato tubers in response to developmental cues. The results indicated that components of the systemin signaling pathway normally found in leaves have been recruited by potato plants to be developmentally regulated to synthesize and accumulate large quantities of storage proteins in tubers.
Narváez-Vasquez
Javier
J
Institute of Biological Chemistry, Washington State University, Pullman 99164-6340, USA.
Ryan
Clarence A
CA
D016960:Agrobacterium tumefaciens / Q000502:physiology; D000596:Amino Acids / Q000378:metabolism; D017796:Caulimovirus / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017343:Genes, Plant; D005813:Genes, Synthetic; D018551:Solanum lycopersicum / Q000235:genetics*; D009584:Nitrogen / Q000378:metabolism; D018515:Plant Leaves; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D018517:Plant Roots; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011480:Protease Inhibitors; D018749:RNA, Plant / Q000235:genetics; D015398:Signal Transduction; D011198:Solanum tuberosum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014170:Transformation, Genetic
D000596:Amino Acids; D018076:DNA, Complementary; D010940:Plant Proteins; D011480:Protease Inhibitors; D018749:RNA, Plant; C034104:proteinase inhibitor II protein, plant; C073705:prosystemin protein, Lycopersicon esculentum; C023578:proteinase inhibitor I (plants); D009584:Nitrogen