BACFinder: genomic localisation of large insert genomic clones based on restriction fingerprinting.
We have developed software that allows the prediction of the genomic location of a bacterial artificial chromosome (BAC) clone, or other large genomic clone, based on a simple restriction digest of the BAC. The mapping is performed by comparing the experimentally derived restriction digest of the BAC DNA with a virtual restriction digest of the whole genome sequence. Our trials indicate that this program identified the genomic regions represented by BAC clones with a degree of accuracy comparable to that of end-sequencing, but at considerably less cost. Although the program has been developed principally for use with Arabidopsis BACs, it should align large insert genomic clones to any fully sequenced genome.
Crowe
Mark L
ML
John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK.
Rana
Debashis
D
Fraser
Fiona
F
Bancroft
Ian
I
Trick
Martin
M
D017360:Arabidopsis / Q000235:genetics; D020137:Base Pair Mismatch / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular; D020451:Contig Mapping / Q000379:methods; D016172:DNA Fingerprinting / Q000379:methods*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D016678:Genome; D018745:Genome, Plant; D015698:Genomic Library; D020161:Physical Chromosome Mapping / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity; D012984:Software
D004262:DNA Restriction Enzymes