In vitro inhibition of pentylenetetrazole and bicuculline-induced epileptiform activity in rat hippocampal pyramidal neurons by aqueous fraction isolated from Delphinium denudatum.
Roots of Delphinium denudatum W. are used for the treatment of epilepsy by traditional healers in subcontinent. Aqueous fraction (AF) isolated from D. denudatum has previously shown significant anticonvulsant activity in in vivo and in vitro models of seizures. We investigated anticonvulsant effects of AF on pentylenetetrazole (PTZ) and bicuculline (BIC)-induced epileptiform activity in primary hippocampal neuronal cultures. Electrophysiological studies on single pyramidal neurons were carried out by using whole-cell current clamp technique. Introduction of AF (0.6 mg/ml) in perfusate blocked PTZ (10 mM) and BIC (100 micro M)-induced epileptiform activity comprising of paroxysmal depolarization shifts (PDS). The PDS were elicited again when AF was removed from perfusate. We conclude that AF contains anticonvulsant compounds that possibly interact with GABA(A) receptor to produce blockade of epileptiform activity. Further studies on isolation of compounds from AF may lead to discovery of new class of anticonvulsants.
Raza
Mohsin
M
Department of Neurology, Medical College of Virginia, Virginia Commonwealth University, P O Box 980599, Richmond, VA 23298-0599, USA. mohsinrazahej@yahoo.com
Shaheen
Farzana
F
Choudhary
M I
MI
Rahman
Atta-ur
AU
Sombati
Sompong
S
DeLorenzo
R J
RJ
D000818:Animals; D000831:Animals, Newborn; D000927:Anticonvulsants / Q000494:pharmacology*; D001640:Bicuculline / Q000494:pharmacology; D002478:Cells, Cultured; D031882:Delphinium; D004827:Epilepsy / Q000139:chemically induced; D018756:GABA Antagonists / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D010433:Pentylenetetrazole / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D017966:Pyramidal Cells / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000927:Anticonvulsants; D018756:GABA Antagonists; D010936:Plant Extracts; D010433:Pentylenetetrazole; D001640:Bicuculline