title: [Expression of macrophage migration inhibitory factor mRNA in Schwann cells].
abstract: OBJECTIVE
  To investigate the expression of macrophage migration inhibitory factor (MIF) mRNA in Schwann cells after peripheral nerve injury and roles of Schwann cells and MIF in macrophages activation and nerve regeneration.
  
  
  METHODS
  Fifty SD rats were divided into 10 groups. One group served as normal control. The rest were anesthetized with 3% sodium pentobarbital (30 - 60 mg/kg, i.p) and sciatic nerves were transected distal to the obturator tendon respectively 1 h, 12 h, 1 d, 3 d, 7 d, 10 d, 14 d, 17 d and 21 d before being killed. Sciatic nerves were resected and connective tissues excised. Schwann cells were obtained by digesting the nerve tissues with trypsin and collagenase. RNA was isolated and reverse-transcription-polymerase chain reaction (RT-PCR) was carried out. cDNA was analyzed by automatic system and the parameters were assessed to define the status of MIF mRNA expression in different groups.
  
  
  RESULTS
  The level of MIF mRNA started to increase 12 h after the nerve transection. The level remained high from day 7 up to 10 after the injury. During the period from days 10 to 21, MIF mRNA decreased slowly to the pre-transection level.
  
  
  CONCLUSIONS
  After peripheral nerve injury, Schwann cells can secrete MIF which may play a pivotal role as an immunomodulatory cytokine in macrophage activation and inflammatory reaction.
authors.affiliation: Institute of Clinical Anatomy, First Military Medical University, Guangzhou 510515, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Qin
authors.forename: Shaohu
authors.initials: S
authors.lastname: Xiong
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Huo
authors.forename: Hua
authors.initials: H
authors.lastname: Liao
authors.forename: Jianyi
authors.initials: J
authors.lastname: Li
authors.forename: Dayong
authors.initials: D
authors.lastname: Liu
mesh_terms: D000818:Animals; D005260:Female; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics*; D008297:Male; D059348:Peripheral Nerve Injuries; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012583:Schwann Cells / Q000378:metabolism*
chemical_list: D008263:Macrophage Migration-Inhibitory Factors; D012333:RNA, Messenger