A site-specific recombinase (RinQ) is required to exert incompatibility towards the symbiotic plasmid of Rhizobium etli.
The replication/partition region of the symbiotic plasmid p42d of Rhizobium etli CE3 is characterized by the presence of the repABC operon. A recombinant plasmid containing this region is able to replicate in a R. etli derivative cured from p42d, with the same stability and copy number shown by the parental plasmid. However, when this construct is introduced into the wild-type strain, instead of exerting incompatibility against the p42d, it forms a stable cointegrate with it. In this paper, we show that a site-specific resolvase, and its action sites are essential factors to displace the symbiotic p42d. We propose a model for this novel incompatibility mechanism.
Quintero
Verónica
V
Programa de Evolución Molecular, centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Morelos, México.
Cevallos
Miguel A
MA
Dávila
Guillermo
G
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004254:DNA Nucleotidyltransferases; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics; D008969:Molecular Sequence Data; D009876:Operon; D011938:Rec A Recombinases / Q000235:genetics; D045522:Recombinases; D012231:Rhizobium / Q000235:genetics* / Q000378:metabolism; D013559:Symbiosis / Q000235:genetics*; D019895:Transposases / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D045522:Recombinases; D004254:DNA Nucleotidyltransferases; D011938:Rec A Recombinases; D019895:Transposases; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V