Laser fluorescence bronchoscopy for detection of fluorescent reporter genes in airway epithelia.
Current methods for detecting successful gene transfer to airway epithelia involve obtaining a sample of the target tissue. This may affect the longevity of expression of the transgene under evaluation. We describe a laser fluorescence bronchoscopic system that can detect the expression of the fluorescent protein, green fluorescence protein (GFP), in the airway of monkeys that have been transfected with adenovirus, without the need for obtaining tissue. This technique will have applications in pre-clinical and clinical studies of gene transfer to airway epithelia and other surface epithelia accessible by endoscopy.
Rooney
C P
CP
Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, IA, USA.
Suter
M
M
McLennan
G
G
Donnelley
M
M
Reinhardt
J
J
Delsing
A
A
Hoffman
E A
EA
Zabner
J
J
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001999:Bronchoscopy / Q000379:methods*; D005453:Fluorescence; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008253:Macaca mulatta; D008297:Male; D011817:Rabbits; D020545:Respiratory Mucosa / Q000378:metabolism*; D014162:Transfection; D019076:Transgenes
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins