title: Formation of a self-assembled phenylboronic acid monolayer and its application toward developing a surface plasmon resonance-based monosaccharide sensor.
abstract: For the surface-optoelectronic study of sugar sensing, we synthesized and characterized dithiobis(4-butyrylamino-m-phenylboronic acid) (DTBA-PBA) as a recognition molecule. DTBA-PBA has a boronic acid group that has been known to form covalently bonded complexes with the 1,2- or 1,3-diol of sugars. A self-assembled monolayer (SAM) of DTBA-PBA was formed on a gold surface and characterized by atomic-force microscopy, Fourier transform infrared reflection absorption spectroscopy, and surface electrochemical measurements. An interaction study between monosaccharides and DTBA-PBA SAM was performed using surface plasmon resonance spectroscopy. The increase in molecular interactions between DTBA-PBA SAM and monosaccharides resulted in an optically induced electron excitation change on the Au surface through a refractive index change of the interfacial recognition layer. This correlation between electron excitation and molecular interaction was measurable at very low monosaccharide concentrations (1.0 x 10(-12)M). DTBA-PBA SAM shows a selective fructose sensing among four kinds of monosaccharides, even in a low concentration range.
authors.affiliation: Department of Sensor Engineering, Graduate School, Kyungpook National University, Taegu 702-701, South Korea.
authors.forename: Minsu
authors.initials: M
authors.lastname: Lee
authors.forename: Tae-Il
authors.initials: TI
authors.lastname: Kim
authors.forename: Kyung-Ho
authors.initials: KH
authors.lastname: Kim
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Myung-Sook
authors.initials: MS
authors.lastname: Choi
authors.forename: Heung-Jin
authors.initials: HJ
authors.lastname: Choi
authors.forename: Kwangnak
authors.initials: K
authors.lastname: Koh
mesh_terms: D015374:Biosensing Techniques; D001897:Boronic Acids / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D006046:Gold / Q000737:chemistry; D018625:Microscopy, Atomic Force; D009005:Monosaccharides / Q000032:analysis* / Q000737:chemistry; D012031:Refractometry; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties
chemical_list: D001897:Boronic Acids; D009005:Monosaccharides; D006046:Gold; C010686:benzeneboronic acid