title: Isolation and characterization of a potato cDNA corresponding to a 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene differentially activated by stress.
abstract: 1-Aminocyclopropane-1-carboxylate (ACC) oxidase enzyme catalyses the final step in ethylene biosynthesis, converting 1-aminocyclopropane-1-carboxylic acid to ethylene. A cDNA clone encoding an ACC oxidase, ST-ACO3, was isolated from potato (Solanum tuberosum L.) by differential screening of a Fusarium eumartii infected-tuber cDNA library. The deduced amino acid sequence exhibited similarity to other ACC oxidase proteins from several plants species. Northern blot analysis revealed that the ST-ACO3 mRNA level increased in potato tubers upon inoculation with F. eumartii, as well as after treatment with salicylic acid and indole-3-acetic acid, suggesting a cross-talk between different signalling pathways involved in the defence response of potato tubers against F. eumartii attack.
authors.affiliation: Instituto de Investigaciones Biológicas-Departamento de Biología, Universidad Nacional de Mar del Plata, Funes 3250, CC 1245, 7600 Mar del Plata, Argentina.
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Zanetti
authors.forename: María Cecilia
authors.initials: MC
authors.lastname: Terrile
authors.forename: Débora
authors.initials: D
authors.lastname: Arce
authors.forename: Andrea Verónica
authors.initials: AV
authors.lastname: Godoy
authors.forename: Blanca San
authors.initials: BS
authors.lastname: Segundo
authors.forename: Claudia
authors.initials: C
authors.lastname: Casalongué
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D018076:DNA, Complementary; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D011198:Solanum tuberosum / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D000594:Amino Acid Oxidoreductases; C059460:1-aminocyclopropane-1-carboxylic acid oxidase