title: Characterization of Sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis.
abstract: Sm14 was the first fatty acid-binding protein homologue identified in helminths. Thereafter, members of the same family were identified in several helminth species, with high aminoacid sequence homology between them. In addition, immune crossprotection was also reported against Fasciola hepatica infection, in animals previously immunized with the Schistosoma mansoni vaccine candidate, r-Sm14. In the present study, data on preliminary sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis of nine different helminth extracts focusing the identification of Sm14 related proteins, is reported. Out of these, three extracts - Ascaris suum (males and females), Echinostoma paraensei, and Taenia saginata - presented components that comigrated with Sm14 in SDS-PAGE, and that were recognized by anti-rSm14 policlonal serum, in Western blotting tests.
authors.affiliation: Laboratório de Esquistossomose Experimental, Departamento de Helmintologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, 21045-900, Brasil.
authors.forename: Nilton
authors.initials: N
authors.lastname: Thaumaturgo
authors.forename: Mônica Magno
authors.initials: MM
authors.lastname: Vilar
authors.forename: Ricardo
authors.initials: R
authors.lastname: Edelenyi
authors.forename: Miriam
authors.initials: M
authors.lastname: Tendler
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D050612:Fatty Acid Transport Proteins; D005260:Female; D015801:Helminth Proteins / Q000737:chemistry / Q000302:isolation & purification*; D008297:Male; D026901:Membrane Transport Proteins; D012550:Schistosoma mansoni / Q000737:chemistry*; D012555:Schistosomiasis mansoni / Q000276:immunology*
chemical_list: D002352:Carrier Proteins; D050612:Fatty Acid Transport Proteins; D015801:Helminth Proteins; D026901:Membrane Transport Proteins; C068709:SM14 protein, Schistosoma mansoni