Demonstration of hepatic Fc receptor function in vivo.
We describe an investigation of the Fc-receptor dependent function of the hepatic component of the mononuclear phagocyte system (MPS) in rats. Erythrocytes sensitized with the rat IgG2b monoclonal antibody (JY1/98) were cleared by the liver in decomplemented splenectomized rats. This immune clearance was Fc-receptor dependent since it was effectively inhibited by immune complexes of bovine serum albumin (BSA) and rabbit-anti-BSA antibody formed either in vivo or in vitro. Immune complexes formed with F (ab')2 fragment of the rabbit anti-BSA antibody had no effect. Heat-aggregated human gamma globulin was virtually without any competitive activity in the Fc-mediated clearance system. Immune complexes inhibited the hepatic clearance of antibody-sensitized erythrocytes but did not have a significant effect on the early rapid removal of erythrocytes pre-coated with antibody and complement.
Yousaf
N
N
Department of Rheumatology, University Hospital of Wales, Cardiff.
Howard
J C
JC
Williams
B D
BD
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000378:metabolism; D002417:Cattle; D003165:Complement System Proteins / Q000378:metabolism; D004546:Elapid Venoms / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G; D008099:Liver / Q000276:immunology*; D008297:Male; D010586:Phagocytes / Q000276:immunology; D011817:Rabbits; D051381:Rats; D011961:Receptors, Fc / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000276:immunology
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D004546:Elapid Venoms; D007074:Immunoglobulin G; D011961:Receptors, Fc; C016310:cobra venom factor; D012710:Serum Albumin, Bovine; D003165:Complement System Proteins