title: Complete nucleotide sequence of the genomic RNA of a French isolate of Pepino mosaic virus (PepMV). Brief report.
abstract: The complete nucleotide sequence of the genomic RNA of a French isolate of Pepino mosaic virus (PepMV) was determined. With a length of 6,425 nucleotides excluding the poly(A) tail at the 3' terminus, the PepMV genome contains five major open reading frames encoding a 164 kDa replicase, triple gene block proteins of 26 kDa, 14 kDa and 9 kDa, and a 25 kDa coat protein. This genome organization, as well as characteristic consensus motifs and high degrees of similarity of PepMV proteins with those of other potexviruses confirmed that PepMV belongs to the Potexvirus genus. Phylogenetic analyses carried out on replicase, triple gene block protein 1 and coat protein amino acid sequences revealed that PepMV is closely related to species Narcissus mosaic virus (NMV), Scallion virus X (SVX), Cymbidium mosaic virus (CymMV), and Potato aucuba mosaic virus (PAMV).
authors.affiliation: Laboratoire National de la Protection des Végétaux, Unité de Virologie des Plantes Herbacées Montfavet, France. cotillon@avignon.inra.fr
authors.forename: A-C
authors.initials: AC
authors.lastname: Cotillon
authors.forename: M
authors.initials: M
authors.lastname: Girard
authors.forename: S
authors.initials: S
authors.lastname: Ducouret
mesh_terms: D020121:5' Untranslated Regions / Q000737:chemistry; D000595:Amino Acid Sequence; D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017863:Potexvirus / Q000145:classification / Q000235:genetics*; D012367:RNA, Viral / Q000737:chemistry*; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics; D032322:Solanum / Q000821:virology*
chemical_list: D020121:5' Untranslated Regions; D036022:Capsid Proteins; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase