Determination of bone and tissue concentrations of teicoplanin mixed with hydroxyapatite cement to repair cortical defects.
A highly specific and sensitive isocratic reversed-phase high performance liquid chromatography (HPLC) method for the determination of the major component of teicoplanin in tissue is reported. Comparing fluorescamine and o-phthalaldehyde (OPA) as derivatizing agents, the derivative formed with the latter exhibits superior fluorescence intensity allowing detection of femtomole quantities. Pretreatment for tissue samples is by solid-phase extraction which uses Bakerbond PolarP C(18) cartridges and gives effective clean up from endogenous by-products. Linearity was given from 0.6 to 100 ng per injection. The coefficient of variation did not exceed 5.8% for both interday and intraday assays. It was found that when bone defects are repaired with a hydroxyapatite-teicoplanin mixture, the antibiotic does not degrade, even when it is in the cement for several months. The stability of teicoplanin in bone cement was determined fluorodensitometrically.
Eggenreich
K
K
Institute of Pharmaceutical Chemistry and Pharmaceutical Technology, Karl-Franzens University, Schubertstrasse 1, A-8010 Graz, Austria.
Zeipper
U
U
Schwendenwein
E
E
Hadju
S
S
Kaltenecker
G
G
Laslo
I
I
Lang
S
S
Roschger
P
P
Vecsei
V
V
Wintersteiger
R
R
D000818:Animals; D001843:Bone Cements / Q000032:analysis*; D001842:Bone and Bones / Q000737:chemistry* / Q000601:surgery; D002484:Cementation / Q000009:adverse effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005450:Fluorescamine; D050723:Fractures, Bone / Q000378:metabolism / Q000601:surgery; D016908:Gram-Positive Bacterial Infections / Q000209:etiology / Q000517:prevention & control; D006882:Hydroxyapatites / Q000032:analysis* / Q000627:therapeutic use; D008422:Materials Testing / Q000379:methods; D011817:Rabbits; D013050:Spectrometry, Fluorescence / Q000379:methods; D017334:Teicoplanin / Q000032:analysis* / Q000627:therapeutic use; D009764:o-Phthalaldehyde
D001843:Bone Cements; D006882:Hydroxyapatites; C475044:hydroxyapatite cement; D005450:Fluorescamine; D017334:Teicoplanin; D009764:o-Phthalaldehyde