Quantitative analysis of osteonectin mRNA in thyroid carcinomas.
Our recent study of the gene expression profile in thyroid carcinoma showed an overexpression of osteonectin mRNA, an extracellular matrix protein, in an anaplastic carcinoma. To confirm this, we measured the expression levels of osteonectin mRNA in 84 thyroid normal and tumor tissues, including five anaplastic carcinomas by realtime quantitative reverse-transcription PCR. Increased expression of osteonectin mRNA was observed in anaplastic carcinoma tissue. However, in five anaplastic carcinoma cell lines, no increase was observed in the expression levels of osteonectin mRNA. These findings suggest the possibility that increased expression of osteonectin mRNA in anaplastic carcinoma tissue may be due to its overexpression in stromal cells, but not in anaplastic carcinoma cells.
Takano
Toru
T
Department of Laboratory Medicine, Osaka University Medical School, Suita, Japan.
Hasegawa
Yukiko
Y
Miyauchi
Akira
A
Matsuzuka
Fumio
F
Yoshida
Hiroshi
H
Kuma
Kanji
K
Hayashi
Nobuyasu
N
Nakamori
Shoji
S
Amino
Nobuyuki
N
D002277:Carcinoma / Q000737:chemistry*; D003199:Computer Systems; D006801:Humans; D015676:Osteonectin / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013961:Thyroid Gland / Q000737:chemistry; D013964:Thyroid Neoplasms / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D015676:Osteonectin; D012333:RNA, Messenger