title: The histidine kinase Hik33 perceives osmotic stress and cold stress in Synechocystis sp PCC 6803.
abstract: The stress imposed on living organisms by hyperosmotic conditions and low temperature appears to be perceived via changes in the physical state of membrane lipids. We compared genome-wide patterns of transcription between wild-type Synechocystis sp. PCC 6803 and cells with a mutation in the histidine kinase Hik33 using a DNA microarray. Our results indicated that Hik33 regulated the expression of both osmostress-inducible and cold-inducible genes. The respective genes that were regulated by Hik33 under hyperosmotic and low-temperature conditions were, for the most part, different from one another. However, Hik33 also regulated the expression of a set of genes whose expression was induced both by osmotic stress and by cold stress. These results indicate that Hik33 is involved in responses to osmotic stress and low-temperature stress but that the mechanisms of the responses differ.
authors.affiliation: Department of Regulation, National Institute for Basic Biology, Okazaki, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Mikami
authors.forename: Yu
authors.initials: Y
authors.lastname: Kanesaki
authors.forename: Iwane
authors.initials: I
authors.lastname: Suzuki
authors.forename: Norio
authors.initials: N
authors.lastname: Murata
mesh_terms: D002455:Cell Division / Q000502:physiology; D003080:Cold Temperature; D000458:Cyanobacteria / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D000071677:Histidine Kinase; D020411:Oligonucleotide Array Sequence Analysis; D009997:Osmotic Pressure; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D011494:Protein Kinases; D000071677:Histidine Kinase