A macrophage-tropic HIV-1 that expresses green fluorescent protein and infects alveolar and blood monocyte-derived macrophages.
We describe the construction of a macrophage-tropic HIV-1 molecular clone, pNLAD8-EGFP, which expresses enhanced green fluorescent protein. We show that NLAD8-EGFP can infect monocyte-derived macrophages as well as alveolar macrophages. NLAD8-EGFP-infected macrophages can be easily and sensitively detected based on the visualization of intracellular green fluorescent protein.
Rich
Elizabeth A
EA
Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
Orenstein
Jan M
JM
Jeang
Kuan-Teh
KT
D015726:Giant Cells; D049452:Green Fluorescent Proteins; D015658:HIV Infections; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D008264:Macrophages / Q000166:cytology / Q000821:virology*; D016676:Macrophages, Alveolar / Q000821:virology*; D008854:Microscopy, Electron; D009000:Monocytes / Q000166:cytology; D012907:Smoking / Q000009:adverse effects
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins