title: Attenuation of spontaneous opiate withdrawal in mice by the anandamide transport inhibitor AM404.
abstract: The endogenous cannabinoid, anandamide, has been shown to attenuate naloxone-precipitated opiate withdrawal in rodents. Here we show that the spontaneous, but not the naloxone-precipitated withdrawal syndrome in morphine-dependent mice is attenuated by the inhibitor of carrier-mediated anandamide transport N-(4-hydroxyphenyl) arachidonylethanolamide (AM404) (2 and 10 mg/kg, i.p.). These results suggest that spontaneous but not opioid antagonist-precipitated withdrawal is associated with dynamic changes in endogenous cannabinoid signaling.
authors.affiliation: Fundación Hospital Carlos Haya, Avda Carlos Haya 82. 7 Planta, Pabellón A., Málaga 29010, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Del Arco
authors.forename: Miguel
authors.initials: M
authors.lastname: Navarro
authors.forename: Ainhoa
authors.initials: A
authors.lastname: Bilbao
authors.forename: Belén
authors.initials: B
authors.lastname: Ferrer
authors.forename: Daniele
authors.initials: D
authors.lastname: Piomelli
authors.forename: Fernando
authors.initials: F
authors.lastname: Rodríguez De Fonseca
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism* / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D063388:Endocannabinoids; D051379:Mice; D009021:Morphine Dependence / Q000378:metabolism*; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D053284:Polyunsaturated Alkamides; D013375:Substance Withdrawal Syndrome / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D001095:Arachidonic Acids; D063388:Endocannabinoids; D009292:Narcotic Antagonists; D053284:Polyunsaturated Alkamides; D009270:Naloxone; C078814:anandamide; C107594:N-(4-hydroxyphenyl)arachidonylamide