[The relationship between donor TNFalpha - 308 (G/A) genotype and recipient acute GVHD in allo-BMT].
OBJECTIVE
To study the relationship between donor TNFalpha - 308 (G/A) genotype and recipient acute graft versus host disease (aGVHD).


METHODS
TNFalpha - 308 (G/A) and TNFalpha - 308 (G/G) genotypes were analyzed by DHPLC and DNA sequence in twenty-one degree III/IV (III/IV) aGVHD patients, and twenty-eight degree 0/I (0/I) aGVHD patients. Serum TNFalpha levels were determined by ELISA.


RESULTS
The frequency of TNFalpha - 308 (G/A) genotype was significantly higher in III/IV aGVHD patients than in 0/I aGVHD patients (8/21 vs 1/28) (P < 0.05); and so did for higher serum TNFalpha levels (P < 0.05). TNFalpha levels were higher in III/IV aGVHD patients than in 0/I aGVHD patients in TNFalpha - 308 (G/G) patients (P < 0.01).


CONCLUSIONS
TNFalpha - 308 (G/A) is positively related to serum TNFalpha levels and most likely contributes to high risk for III/IV aGVHD.
Wang
Jingbo
J
Institute of Hematology and People's Hospital, Peking University, Beijing 100044, China.
Pan
Kaifeng
K
Li
Dan
D
Lu
Daopei
D
D000208:Acute Disease; D001483:Base Sequence; D001782:Blood Donors; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005787:Gene Frequency; D005838:Genotype; D006086:Graft vs Host Disease / Q000097:blood / Q000235:genetics*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
D014409:Tumor Necrosis Factor-alpha; D004247:DNA