Tyrosine 92 of aristolochene synthase directs cyclisation of farnesyl pyrophosphate.
A mutant of Aristolochene Synthase (AS), in which Tyr 92 was replaced by Val, produced the alicyclic beta-(E)-farnesene as the major product, indicating that cyclisation of FPP is controlled by Tyr 92 in AS.
Calvert
Melanie J
MJ
School of Chemical Sciences, University of Birmingham, Edgbaston, Birmingham, UK B15 2TT.
Taylor
Susan E
SE
Allemann
Rudolf K
RK
D019943:Amino Acid Substitution; D003500:Cyclization / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D007535:Isomerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D011106:Polyisoprenyl Phosphates / Q000378:metabolism*; D012717:Sesquiterpenes / Q000378:metabolism; D014443:Tyrosine / Q000494:pharmacology
D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; C062671:beta-farnesene; D014443:Tyrosine; C004808:farnesyl pyrophosphate; D007535:Isomerases; C060050:aristolochene synthase