title: Proinflammatory cytokines but not acute phase serum amyloid A or C-reactive protein, downregulate paraoxonase 1 (PON1) expression by HepG2 cells.
abstract: The expression of paraoxonase1 (PON1) during inflammation has been investigated in vitro. The alteration of steady state PON1 mRNA in HepG2 cells by interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha), was investigated relative to acute-phase serum amyloid A (A-SAA) mRNA. PON1 mRNA expression by HepG2 cells was decreased within three hours of stimulation by IL-1beta or TNF-alpha. Relative to PON1 mRNA expression, the pattern of steady state A-SAA mRNA expression was altered reciprocally and inversely by IL-1beta. These findings suggested that the decrease in serum PON activity after abdominal surgery in our previous clinical study may be ascribed to a decrease in steady state PON1 mRNA expression by liver with proinflammatory cytokines.
authors.affiliation: Second Department of Internal Medicine, Kochi Medical School, Nankoku, Japan. kumony@kochi-ms.ac.jp
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Kumon
authors.forename: Yuh
authors.initials: Y
authors.lastname: Nakauchi
authors.forename: Tadashi
authors.initials: T
authors.lastname: Suehiro
authors.forename: Tomoko
authors.initials: T
authors.lastname: Shiinoki
authors.forename: Noriyasu
authors.initials: N
authors.lastname: Tanimoto
authors.forename: Mari
authors.initials: M
authors.lastname: Inoue
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nakamura
authors.forename: Kozo
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Jean D
authors.initials: JD
authors.lastname: Sipe
mesh_terms: D001053:Apolipoproteins / Q000494:pharmacology; D043303:Aryldialkylphosphatase; D002097:C-Reactive Protein / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000378:metabolism; D002352:Carrier Proteins; D002373:Cyclic AMP Receptor Protein / Q000494:pharmacology; D016207:Cytokines / Q000276:immunology* / Q000494:pharmacology; D015536:Down-Regulation; D004950:Esterases / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D000685:Serum Amyloid A Protein / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D001053:Apolipoproteins; D002352:Carrier Proteins; D002373:Cyclic AMP Receptor Protein; D016207:Cytokines; D000685:Serum Amyloid A Protein; D002097:C-Reactive Protein; D004950:Esterases; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human