Fluorescence quenching of dimeric and monomeric forms of yeast hexokinase (PII): effect of substrate binding steady-state and time-resolved fluorescence studies.
Fluorescence quenching studies on the PII isoenzyme of yeast hexokinase have been performed using charged as well as polar uncharged quenchers. In both 'open' (i.e. in the absence of glucose) and 'closed' (i.e. in the presence of glucose) forms of the enzyme, bimolecular quenching rate constant (kq) for acrylamide is significantly larger than that of KI, indicating that all the tryptophans are not fully exposed to the solvent. Overall accessibility of tryptophans towards KI was greater in the presence of glucose than in the absence of glucose. At high ionic strength, the value of bimolecular quenching rate constant (kq) for KI did not change suggesting that the average environment of the accessible tryptophan residue(s) is almost neutral. Quenching by KI is dynamic in nature. Accessibility of tryptophans towards acrylamide at concentration > or = 0.2 M was more in the 'open' form of the enzyme than that observed in the 'closed' form whereas at concentration < or = 0.2 M no significant difference in the extent of quenching was observed. It is reasonable to conclude that glucose induced conformational change leads some tryptophan residue(s) to be more exposed and at the same time some tryptophan residue(s) in the hydrophobic region become more buried. Dimeric and monomeric forms of the enzyme behave similarly towards the quenching by acrylamide. In the unfolded state, the accessibility of tryptophans was considerably higher for both the quenchers. Temperature dependent study and the fluorescence lifetime data indicate that the mechanism of quenching by acrylamide is primarily dynamic in nature.
Maity
Haripada
H
Tata Institute of Fundamental Research, Colaba, Mumbai, India.
Jarori
Gotam K
GK
D020106:Acrylamide / Q000737:chemistry; D003461:Crystallography / Q000379:methods; D019281:Dimerization; D004789:Enzyme Activation; D005947:Glucose / Q000737:chemistry*; D006593:Hexokinase / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D007527:Isoenzymes / Q000737:chemistry; D046911:Macromolecular Substances; D008968:Molecular Conformation; D011193:Potassium Iodide / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013379:Substrate Specificity; D014364:Tryptophan / Q000737:chemistry*; D015003:Yeasts / Q000201:enzymology*
D007527:Isoenzymes; D046911:Macromolecular Substances; D011193:Potassium Iodide; D020106:Acrylamide; D014364:Tryptophan; D006593:Hexokinase; D005947:Glucose