title: A motif of a microbial starch-binding domain found in human genethonin.
abstract: The sequence of the starch-binding domain present in 10% of amylolytic enzymes of microbial origin and classified as the carbohydrate-binding module family 20, was identified in the equivalent part of sequence of human genethonin, a skeletal muscle protein of unknown function. The sequence identity between the starch-binding domain from Bacillus sp. strain 1011 cyclodextrin glucanotransferase and the corresponding segment of human genethonin was higher than 28%. The amino acid residues known to be involved in the raw starch binding were found to be conserved in the genethonin sequence. The three-dimensional structure of the genethonin 'starch-binding domain' was modelled and its eventual function briefly discussed.
authors.affiliation: Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, SK-84251 Bratislava, Slovakia. stefan.janecek@savba.sk
authors.forename: Stefan
authors.initials: S
authors.lastname: Janecek
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics*; D017124:Conserved Sequence; D030562:Databases, Protein; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics*; D017434:Protein Structure, Tertiary / Q000235:genetics; D016415:Sequence Alignment / Q000379:methods*; D020539:Sequence Analysis, Protein / Q000379:methods; D013045:Species Specificity; D013213:Starch / Q000378:metabolism
chemical_list: D005087:Glucan 1,4-alpha-Glucosidase; D005964:Glucosyltransferases; D008565:Membrane Proteins; D009124:Muscle Proteins; D013213:Starch; C116148:STBD1 protein, human; C019963:cyclomaltodextrin glucanotransferase