title: Expression and folding of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm.
abstract: In this review, we summarize some of our results on folding and directed evolution of an antibody fragment in Escherichia coli cytoplasm. We will also discuss some attempts to construct other antibodies active in this cellular compartment.
authors.affiliation: CNRS UMR5094, Faculté de Pharmacie, Montpellier, France.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Laden
authors.forename: Pascal
authors.initials: P
authors.lastname: Philibert
authors.forename: François
authors.initials: F
authors.lastname: Torreilles
authors.forename: Martine
authors.initials: M
authors.lastname: Pugnière
authors.forename: Pierre
authors.initials: P
authors.lastname: Martineau
mesh_terms: D003593:Cytoplasm / Q000378:metabolism*; D019020:Directed Molecular Evolution; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000378:metabolism*; D017510:Protein Folding
chemical_list: D007128:Immunoglobulin Fragments; C055966:immunoglobulin Fv