Group II introns: highly specific endonucleases with modular structures and diverse catalytic functions.
Group II introns are large catalytic RNAs with a remarkable repertoire of reactions. Here we present construct designs and protocols that were used to develop a set of kinetic frameworks for studying the structure and reaction mechanisms of group II introns and ribozymes derived from them. In addition, we discuss application of these systems to structure/function analysis of the ai5gamma group II intron.
Fedorova
Olga
O
Department of Biochemistry and Molecular Biophysics, 630 West 168 Street, Box 36, Columbia University, New York, NY 10032, USA.
Su
Linhui Julie
LJ
Pyle
Anna Marie
AM
D002384:Catalysis; D006868:Hydrolysis; D007438:Introns; D007700:Kinetics; D009690:Nucleic Acid Conformation; D012326:RNA Splicing; D016337:RNA, Catalytic / Q000737:chemistry* / Q000502:physiology; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D016337:RNA, Catalytic