title: Responses in the respiratory tract of rats following exposure to sulphuric acid aerosols for 5 or 28 days.
abstract: Sulphuric acid mists have been classified by the International Agency for Research on Cancer as being carcinogenic to humans based on epidemiological findings of respiratory tract tumours. To determine if early changes in the respiratory tract following exposure to sulphuric acid (H(2)SO(4)) aerosols are consistent with the possible development of tumours after extended periods of exposure, groups of female rats were exposed to respirable aerosols of H(2)SO(4) at target concentrations of 0, 0.2, 1.0 or 5.0 mg m(-3) for 6 h per day for either 5 days or for 5 days a week over a 28-day period. Additional groups exposed to 0 or 5.0 mg m(-3) over the 28-day period were retained after exposure for 4 or 8 weeks to assess recovery. Histopathological examinations and quantitative cell proliferation measurements were conducted on the nasal passages, larynx and lung. Achieved concentrations were 0.3, 1.38 and 5.52 mg m(-3) H(2)SO(4). Histological and cell proliferative changes were confined to the larynx and no effects were seen in the nasal passages or lungs. At the two highest concentrations, squamous metaplasia accompanied by significant cell proliferation was apparent after 5 and 28 days of exposure and there was a reduction in the severity of the pathological changes following the recovery periods. No effects were seen at 0.3 mg m(-3) after 5 days of exposure and only minimal metaplastic change was seen after 28 days in a few animals and was not accompanied by cell proliferation. The toxicological relevance of these findings is discussed.
authors.affiliation: Syngenta CTL, Alderley Park, Macclesfield, Cheshire SK10 4TJ, UK. Joanne.Kilgour@syngenta.com
authors.forename: Joanne D
authors.initials: JD
authors.lastname: Kilgour
authors.forename: John
authors.initials: J
authors.lastname: Foster
authors.forename: Anthony
authors.initials: A
authors.lastname: Soames
authors.forename: David G
authors.initials: DG
authors.lastname: Farrar
authors.forename: Paul M
authors.initials: PM
authors.lastname: Hext
mesh_terms: D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005260:Female; D007830:Larynx / Q000187:drug effects / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D008679:Metaplasia / Q000139:chemically induced / Q000473:pathology; D009296:Nasal Cavity / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012137:Respiratory System / Q000187:drug effects* / Q000473:pathology*; D013464:Sulfuric Acids / Q000008:administration & dosage / Q000633:toxicity*; D013997:Time Factors
chemical_list: D000336:Aerosols; D013464:Sulfuric Acids; C033158:sulfuric acid