Intestinal bicarbonate secretion by marine teleost fish--why and how?
Intestinal fluids of most marine teleosts are alkaline (pH 8.4-9.0) and contain high levels of HCO(3)(-) equivalents (40-130 mM) which are excreted at a significant rate (>100 microEq kg(-1) h(-1)). Recent research reveals the following about this substantial HCO(3)(-) secretion: (1) It is not involved in acid-base regulation or neutralisation of stomach acid, but increases in parallel with drinking rate at elevated ambient salinities suggesting a role in osmoregulation; (2) In species examined so far, all sections of the intestine can secrete bicarbonate; (3) The secretion is dependent on mucosal Cl(-), sensitive to mucosal DIDS, and immuno-histochemistry indicates involvement of an apical Cl(-)/HCO(3)(-) exchanger. In addition, hydration of CO(2) via carbonic anhydrase in combination with proton extrusion appears to be essential for bicarbonate secretion. The mode of proton extrusion is currently unknown but potential mechanisms are discussed. One consequence of the luminal alkalinity and high bicarbonate concentrations is precipitation of calcium and magnesium as carbonate complexes. This precipitation is hypothesised to reduce the osmolality of intestinal fluids and thus play a potential role in water absorption and osmoregulation. The present studies on European flounder reveal that elevated luminal calcium (but not magnesium) concentrations stimulate intestinal bicarbonate secretion both acutely and chronically, in vitro and in vivo. At the whole animal level, the result of this elevated bicarbonate secretion was increased calcium precipitation with an associated reduction in the osmolality of rectal fluids and plasma. These observations suggest direct functional links between intestinal bicarbonate secretion, divalent cation precipitation and osmoregulation in marine teleost fish.
Wilson
Rod W
RW
School of Biological Sciences, Hatherly Laboratories, University of Exeter, Exeter, UK. r.w.wilson@exeter.ac.uk
Wilson
Jonathan M
JM
Grosell
Martin
M
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000378:metabolism; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism*; D002119:Calcium Carbonate / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D002413:Cations, Divalent; D027963:Chloride-Bicarbonate Antiporters / Q000032:analysis / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D004326:Drinking; D005399:Fishes / Q000097:blood / Q000378:metabolism*; D005432:Flounder; D005455:Fluorescent Antibody Technique; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism*; D008274:Magnesium / Q000737:chemistry / Q000378:metabolism; D009994:Osmolar Concentration; D010477:Perfusion; D012623:Seawater; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis / Q000378:metabolism; D014882:Water-Electrolyte Balance
D001639:Bicarbonates; D002413:Cations, Divalent; D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides; C005479:magnesium carbonate; D000254:Sodium-Potassium-Exchanging ATPase; D002119:Calcium Carbonate; D008274:Magnesium; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid