Rat hindlimb unloading: Soleus and Extensor Digitorum Longus histochemistry, mitochondrial DNA content and mitochondrial DNA deletions.
Mitochondrial phenotypic alterations, mitochondrial DNA content and mitochondrial DNA deletions in a slow, Soleus, and a fast, Extensor Digitorum Longus, skeletal muscle of 3- and 15-month-old hindlimb suspended rats have been studied. Cytochrome c oxidase-negative fibers appeared after unloading in all examined animals and their percentage increased with increasing unloading time. After 14 days of suspension the mitochondrial DNA content did not change in 3-month-old but decreased significantly in 15-month-old rats. Soleus was much more affected by unloading than Extensor Digitorum Longus. The mitochondrial DNA deletion of 4834 bp as well as other mtDNA deletions, researched with Long Distance-PCR, were absent in both studied muscles before and after unloading.
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Department of Biochemistry and Molecular Biology, University of Bari, Italy.
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D000818:Animals; D001284:Atrophy; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000235:genetics*; D017353:Gene Deletion; D019417:Hindlimb Suspension / Q000502:physiology*; D006651:Histocytochemistry; D018482:Muscle, Skeletal / Q000737:chemistry / Q000473:pathology / Q000502:physiology*; D051381:Rats
D004272:DNA, Mitochondrial