Expression of inorganic phosphate/vesicular glutamate transporters (BNPI/VGLUT1 and DNPI/VGLUT2) in the cerebellum and precerebellar nuclei of the rat.
Expression of inorganic phosphate/vesicular glutamate transporters (BNPI/VGLUT1 and DNPI/VGLUT2) was studied in the cerebellum and precerebellar nuclei of rats using immunohistochemistry and in situ hybridization. DNPI/VGLUT2-stained mossy fibers were principally seen in the vermis (lobules I and VIII-X) and flocculus, whereas BNPI/VGLUT1-stained mossy fibers were localized throughout the cortex. Some vermal and floccular mossy fibers were stained for both transporters. High levels of DNPI/VGLUT2 mRNA hybridization signals were demonstrated in many neurons throughout the vestibular nuclear complex as well as the lateral reticular, external cuneate, inferior olivary and deep cerebellar nuclei. Significant BNPI/VGLUT1 mRNA signals were demonstrated in the lateral reticular nucleus and vestibular nuclear complex but not in the inferior olivary nucleus, indicating that climbing fibers have DNPI/VGLUT2 only. These results show that DNPI/VGLUT2 is expressed preferentially to vestibulo-, reticulo- and cuneocerebellar neurons, some of which also possess BNPI/VGLUT1, suggesting some differential and co-operative functions between DNPI/VGLUT2 and BNPI/VGLUT1 in the cerebellum.
Hisano
Setsuji
S
Laboratory of Neuroendocrinology, Institute of Basic Medical Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8575, Japan. shisano@md.tsukuba.ac.jp
Sawada
Kazuhiko
K
Kawano
Michihiro
M
Kanemoto
Mizuki
M
Xiong
Guoxiang
G
Mogi
Koichi
K
Sakata-Haga
Hiromi
H
Takeda
Jun
J
Fukui
Yoshihiro
Y
Nogami
Haruo
H
D000818:Animals; D001933:Brain Stem / Q000166:cytology / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression / Q000502:physiology; D018698:Glutamic Acid / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D026901:Membrane Transport Proteins; D009412:Nerve Fibers / Q000378:metabolism* / Q000648:ultrastructure; D009434:Neural Pathways / Q000166:cytology / Q000378:metabolism*; D009847:Olivary Nucleus / Q000166:cytology / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012154:Reticular Formation / Q000166:cytology / Q000378:metabolism; D009435:Synaptic Transmission / Q000502:physiology; D050598:Vesicular Glutamate Transport Protein 1; D050599:Vesicular Glutamate Transport Protein 2; D033921:Vesicular Transport Proteins; D014726:Vestibular Nuclei / Q000166:cytology / Q000378:metabolism
D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D010710:Phosphates; D012333:RNA, Messenger; C493475:Slc17a6 protein, rat; C493466:Slc17a7 protein, rat; D050598:Vesicular Glutamate Transport Protein 1; D050599:Vesicular Glutamate Transport Protein 2; D033921:Vesicular Transport Proteins; D018698:Glutamic Acid