title: Mitochondria-rich cells as experimental model in studies of epithelial chloride channels.
abstract: The mitochondria-rich (mr) cell of amphibian skin epithelium is differentiated as a highly specialised pathway for passive transepithelial transport of chloride. The apical membrane of mr cells expresses several types of Cl(-) channels, of which the function of only two types has been studied in detail. (i) One type of channel is gated by voltage and external chloride concentration. This intriguing type of regulation leads to opening of channels only if [Cl(-)](o) is in the millimolar range and if the electrical potential is of a polarity that secures an inwardly directed net flux of this ion. Reversible voltage activations of the conductance proceed with long time constants, which depend on V in such a way that the rate of conductance activation increases when V is clamped at more negative values (serosal bath grounded). The gating seems to involve processes that are dependent on F-actin localised in the submembrane domain in the neck region of the flask-shaped mr cell. (ii) The other identified Cl(-) pathway of mr cells is mediated by small-conductance apical CFTR chloride channels as concluded from its activation via beta-adrenergic receptors, ion selectivity, genistein stimulation and inhibition by glibenclamide. bbCFTR has been cloned, and immunostaining has shown that the gene product is selectively expressed in mr cells. There is cross-talk between the two pathways in the sense that activation of the conductance of the mr cell by voltage clamping excludes activation via receptor occupation, and vice versa. The mechanism of this cross-talk is unknown.
authors.affiliation: Zoophysiological Laboratory, August Krogh Institute, University of Copenhagen, Universitetsparken 13, DK-2100 Copenhagen Ø, Denmark.
authors.forename: Niels J
authors.initials: NJ
authors.lastname: Willumsen
authors.forename: Jan
authors.initials: J
authors.lastname: Amstrup
authors.forename: Nadja
authors.initials: N
authors.lastname: Møbjerg
authors.forename: Ase
authors.initials: A
authors.lastname: Jespersen
authors.forename: Poul
authors.initials: P
authors.lastname: Kristensen
authors.forename: E Hviid
authors.initials: EH
authors.lastname: Larsen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D002023:Bufo bufo; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D018118:Chloride Channels / Q000378:metabolism*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000737:chemistry / Q000378:metabolism; D004553:Electric Conductivity; D004734:Energy Metabolism; D004848:Epithelium / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D008564:Membrane Potentials; D008928:Mitochondria; D023421:Models, Animal; D008969:Molecular Sequence Data; D018408:Patch-Clamp Techniques; D016415:Sequence Alignment; D012867:Skin / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D018118:Chloride Channels; D019005:Cystic Fibrosis Transmembrane Conductance Regulator