The complete nucleotide sequence of the PanAsia strain of foot-and-mouth disease virus isolated in Japan.
The complete nucleotide sequence of the foot-and-mouth disease virus (FMDV) O/JPN/2000 strain, the PanAsia strain, was determined by cycle sequencing and primer walking. The 5' end of the genome upstream from homopolymeric poly(C) tract (S-fragment) was 367 nucleotides in length, and the remainder of the genome (L-fragment), excepting the poly(A) tail, was 7808 nucleotides. The L-fragment contains a single open reading frame of 6996 nucleotides terminating at a UAA codon 96 bases from the 3' poly(A) sequence. Comparison of sequences shows that the length of the structural and non-structural protein coding regions are identical to those in the O1/Kaufbeuren strain, and no striking differences such as deletion or insertion were observed between them.
Kanno
Toru
T
Department of Exotic Diseases, National Institute of Animal Health, Kodaira, Tokyo, Japan. kannot@affrc.go.jp
Yamakawa
Makoto
M
Yoshida
Kazuo
K
Sakamoto
Kenichi
K
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D005536:Foot-and-Mouth Disease / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D007564:Japan; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA