title: Detection of a double-stranded RNA virus from a strain of the violet root rot fungus Helicobasidium mompa Tanaka.
abstract: Three double-stranded (ds) RNA species (ca. 1.30, 1.27 and 1.23 x 106) were isolated by CF-11 cellulose chromatography from a strain of the violet root rot fungus Helicobasidium mompa recovered from apple roots. Purified virion preparations contained isometric particles about 25 nm in diameter, and also the same three species of dsRNA isolated from total extracts by CF-11 cellulose chromatography. The molecular mass of the coat protein was about 67 K when estimated by SDS-PAGE. The largest dsRNA (referred to as dsRNA1) contains a single, long open reading frame of 1794 nucleotides that encodes a putative polypeptide containing 598 amino acid residues with a molecular mass of 69.9 K. This polypeptide contains amino acid sequence motifs conserved in putative RNA-dependent RNA polymerases of RNA viruses. Phylogenetic analysis revealed similarities to RNA-dependent RNA polymerases from Atkinsonella hypoxylon 2H virus, a member of the family Partitiviridae.
authors.affiliation: National Institute of Fruit Tree Science, Fujimoto, Tsukuba, Japan. hideki@affrc.go.jp
authors.forename: Hideki
authors.initials: H
authors.lastname: Osaki
authors.forename: Kinya
authors.initials: K
authors.lastname: Nomura
authors.forename: Toru
authors.initials: T
authors.lastname: Iwanami
authors.forename: Satoko
authors.initials: S
authors.lastname: Kanematsu
authors.forename: Ikuko
authors.initials: I
authors.lastname: Okabe
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Matsumoto
authors.forename: Atsuko
authors.initials: A
authors.lastname: Sasaki
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ohtsu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001487:Basidiomycota / Q000821:virology*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D027845:Malus; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012328:RNA Viruses / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D012330:RNA, Double-Stranded / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017422:Sequence Analysis, DNA; D014197:Trees / Q000382:microbiology; D032413:Viola / Q000382:microbiology*; D014771:Virion / Q000302:isolation & purification / Q000648:ultrastructure
chemical_list: D018076:DNA, Complementary; D012330:RNA, Double-Stranded; D012324:RNA-Dependent RNA Polymerase