Clinical application of the serum 1,5-anhydroglucitol assay method using glucose 3-dehydrogenase.
We attempted to develop a novel serum 1,5-anhydroglucitol (1,5-AG) assay kit using glucose 3-dehydrogenase (G3DH) from Halomonas sp. alpha-15 strain. The major advantages of this method are that the 1,5-AG detection requires a very small amount of G3DH, and the enzyme catalyzes a simple reaction. The analytical performances were acceptable for clinical use operated with a clinical automatic analyzer. The correlation with a commercial assay kit against sera of healthy volunteers was y=0.975x+0.008, r=0.993, Sylx=1.32 microg/mL. However, sham-negative specimens were observed in the validation of this method using specimens from hospital patients.
Hamafuji
Tetsuro
T
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Tokyo, Japan. hamafuji@irc-net.co.jp
Tsugawa
Wakako
W
Sode
Koji
K
D001774:Blood Chemical Analysis; D003847:Deoxyglucose / Q000097:blood*; D004305:Dose-Response Relationship, Drug; D005069:Evaluation Studies as Topic; D005948:Glucose Dehydrogenases / Q000378:metabolism*; D006801:Humans; D010949:Plasma / Q000737:chemistry* / Q000378:metabolism; D015203:Reproducibility of Results; D013778:Tetrazolium Salts / Q000378:metabolism
C110656:2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium; D013778:Tetrazolium Salts; C006584:1,5-anhydroglucitol; D003847:Deoxyglucose; D005948:Glucose Dehydrogenases