Expression of the 12-oxophytodienoic acid 10,11-reductase gene in the compatible interaction between pea and fungal pathogen.
Suppressors produced by Mycosphaerella pinodes are glycopeptides to block pea defense responses induced by elicitors. A clone, S64, was isolated as cDNA for suppressor-inducible gene from pea epicotyls. The treatment of pea epicotyls with suppressor alone induced an increase of S64 mRNA within 1 h, and it reached a maximum level at 3 h after treatment. The induction was not affected by application of the elicitor, indicating that the suppressor has a dominant action to regulate S64 gene expression. S64 was also induced by inoculation with a virulent pathogen, M. pinodes, but not by inoculation with a non-pathogen, Ascochyta rabiei, nor by treatment with fungal elicitor. The deduced structure of S64 showed high homology to 12-oxophytodienoic acid reductase (OPR) in Arabidopsis thaliana. A recombinant protein derived from S64 had OPR activity, suggesting compatibility-specific activation of the octadecanoid pathway in plants. Treatment with jasmonic acid (JA) or methyl jasmonic acid, end products of the octadecanoid pathway, inhibited the elicitor-induced accumulation of PAL mRNA in pea. These results indicate that the suppressor-induced S64 gene expression leads to the production of JA or related compounds, which might contribute to the establishment of compatibility by inhibiting the phenylpropanoid biosynthetic pathway.
Ishiga
Yasuhiro
Y
Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, Tsushima-naka, 1-1-1 Okayama, 700-8530 Japan.
Funato
Akiko
A
Tachiki
Tomoyuki
T
Toyoda
Kazuhiro
K
Shiraishi
Tomonori
T
Yamada
Tetsuji
T
Ichinose
Yuki
Y
D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology; D003890:Desiccation; D005658:Fungi / Q000254:growth & development*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors; D054883:Oxylipins; D018532:Pisum sativum / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology; D010650:Phenylalanine Ammonia-Lyase / Q000187:drug effects / Q000378:metabolism; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000737:chemistry / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C011006:jasmonic acid; C072239:methyl jasmonate; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C106360:12-oxophytodienoate reductase; D009247:NADH, NADPH Oxidoreductases; D010650:Phenylalanine Ammonia-Lyase