title: Interleukin-12 p40 gene (IL12B) 3'-untranslated region polymorphism is associated with susceptibility to atopic dermatitis and psoriasis vulgaris.
abstract: Interleukin-12 (IL-12) is believed to play an important role in inducing Th1-type cytokine profiles. Atopic dermatitis (AD) and psoriasis vulgaris (PsV) are considered to be Th2 and Th1 type disease, respectively. The IL-12 p40 subunit gene (IL12B) is located at chromosome 5q31-33 and linkage findings of AD on 5q31 were reported. Recently single nucleotide polymorphism (SNP) (1188A/C) of IL12B has been reported. In function, it has been reported that this SNP is associated with IL12B mRNA expression levels. To learn whether this SNP is associated with susceptibility to AD or PsV, we investigated the genotype and allele frequencies of the SNP in AD patients, in PsV patients and in controls, examining 164 AD patients, 143 PsV patients and 100 healthy individuals in Japanese population. Genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism method. The A allele was decreased in AD patients (40.9%, p = 0.031) and increased in PsV patients (60.1%, p = 0.035) compared with controls (50.5%). This suggests that IL12B SNP is associated with susceptibility to AD and PsV, presumably by affecting the Th1/Th2 balance.
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan. ytsun-tky@umin.ac.jp
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Tsunemi
authors.forename: Hidehisa
authors.initials: H
authors.lastname: Saeki
authors.forename: Koichiro
authors.initials: K
authors.lastname: Nakamura
authors.forename: Takashi
authors.initials: T
authors.lastname: Sekiya
authors.forename: Koichi
authors.initials: K
authors.lastname: Hirai
authors.forename: Hideki
authors.initials: H
authors.lastname: Fujita
authors.forename: Noriko
authors.initials: N
authors.lastname: Asano
authors.forename: Megumi
authors.initials: M
authors.lastname: Kishimoto
authors.forename: Yuka
authors.initials: Y
authors.lastname: Tanida
authors.forename: Takashi
authors.initials: T
authors.lastname: Kakinuma
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mitsui
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Tada
authors.forename: Motoshi
authors.initials: M
authors.lastname: Wakugawa
authors.forename: Hideshi
authors.initials: H
authors.lastname: Torii
authors.forename: Mayumi
authors.initials: M
authors.lastname: Komine
authors.forename: Akihiko
authors.initials: A
authors.lastname: Asahina
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Tamaki
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000483:Alleles; D003876:Dermatitis, Atopic / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D018664:Interleukin-12 / Q000235:genetics*; D053762:Interleukin-12 Subunit p40; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D011565:Psoriasis / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C500879:IL12B protein, human; D053762:Interleukin-12 Subunit p40; D018664:Interleukin-12