Cryopreserved cell monolayers for rapid detection of herpes simplex virus and influenza virus.
Cryopreserved cell monolayers are a new cell culture technology intended to ensure the availability of cells in the laboratory for virus detection. Two cryopreserved cell monolayers, ELVIS for the detection of herpes simplex virus (HSV) and R-Mix for the detection of influenza virus, were evaluated. The results indicated that fresh and cryopreserved cell monolayers are comparable in sensitivity for the detection of HSV and influenza virus. The cells retain the same level of sensitivity for up to 4 months at -80 degrees C.
Huang
Yung T
YT
Department of Pathology, University Hospitals of Cleveland, Case Western Reserve University, Cleveland, Ohio 44106, USA. yth@po.cwru.edu
Yan
Huimin
H
Sun
Yan
Y
Jollick
Joseph A
JA
Baird
Heather
H
D000818:Animals; D002478:Cells, Cultured; D015925:Cryopreservation; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D009980:Influenza A virus / Q000254:growth & development / Q000302:isolation & purification*; D009981:Influenza B virus / Q000254:growth & development / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013997:Time Factors; D014776:Virus Cultivation / Q000379:methods*
D011933:Reagent Kits, Diagnostic