title: Attenuation of fungal infection in thermoregulating Locusta migratoria is accompanied by changes in hemolymph proteins.
abstract: Hemolymph proteins in the locust, Locusta migratoria migratorioides infected with the fungus Metarhizium anisopliae var acridum were analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Under conditions that allowed locusts to thermoregulate, 2 proteins, ITB1 (ca. 18kDa) and ITB2 (ca. 13kDa) were induced 48h post inoculation. In contrast, under non-thermoregulating conditions, only 1 band, INTB1 (ca. 18kDa) was induced with similar molecular mass to ITB1. ITB1 and ITB2 were N-terminally sequenced but showed little homology to known proteins. The induction of hemolymphal proteins in infected, thermoregulating locusts and implication in insect immune defence are discussed.
authors.affiliation: Département de Phytologie, Université Laval, Sainte-Foy, G1K 7P4, Québec, Qc, Canada.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Ouedraogo
authors.forename: Andrena
authors.initials: A
authors.lastname: Kamp
authors.forename: Mark S
authors.initials: MS
authors.lastname: Goettel
authors.forename: Jacques
authors.initials: J
authors.lastname: Brodeur
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bidochka
mesh_terms: D000818:Animals; D001833:Body Temperature Regulation; D006110:Grasshoppers / Q000382:microbiology* / Q000502:physiology*; D006458:Hemolymph / Q000378:metabolism*; D009181:Mycoses / Q000503:physiopathology*; D011506:Proteins / Q000378:metabolism
chemical_list: D011506:Proteins