[Effects of selenium and zinc on rat renal apoptosis and change of cell cycle induced by fluoride].
OBJECTIVE
This study was conducted to study the effects of sodium fluoride (NaF) on rat renal apoptosis and proliferation, the antagonistic effect of selenium-zinc preparation (Se-Zn) to NaF.


METHODS
Wistar rats were provided with distilled water containing NaF (50 mg/L) and administered by gavage with different dosed of Se-Zn for six months. Kidney cell apoptosis and the cell cycle of proliferation were detected by TUNEL (TdT-mediated dUTP Nick End Labelling) and flow cytometry.


RESULTS
NaF caused rat renal apoptosis, reduce the cell number of G(2)/M period in cell cycle and decrease the relative content of DNA significantly. Se-Zn inhibited the effects of NaF on apoptosis and increased the cell number of G(2)/M period in cell cycle, but failed to increase relative content of DNA.


CONCLUSIONS
It was suggested that NaF could induce apoptosis and change the cell cycle in rat renal cells and Se-Zn could antagonize apoptosis and the changes of cell cycle induced by NaF.
Yu
Rian
R
Department of Environmental Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Xia
Tao
T
Wang
Aiguo
A
Chen
Xuemin
X
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D004336:Drug Antagonism; D005434:Flow Cytometry; D020287:In Situ Nick-End Labeling; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D012643:Selenium / Q000494:pharmacology*; D012969:Sodium Fluoride / Q000494:pharmacology*; D015032:Zinc / Q000494:pharmacology*
D012969:Sodium Fluoride; D004247:DNA; D012643:Selenium; D015032:Zinc