title: Gamma-tubulin37C and gamma-tubulin ring complex protein 75 are essential for bicoid RNA localization during drosophila oogenesis.
abstract: bicoid (bcd) RNA localization requires the activity of exuperantia and swallow at sequential steps of oogenesis and is microtubule dependent. In a genetic screen, we identified two novel genes essential for bcd RNA localization. They encode maternal gamma-Tubulin37C (gammaTub37C) and gamma-tubulin ring complex protein 75 (Dgrip75), both of which are gamma-tubulin ring complex components. Mutations in these genes specifically affect bcd RNA localization, whereas other microtubule-dependent processes during oogenesis are not impaired. This provides direct evidence that a subset of microtubules organized by the gamma-tubulin ring complex is essential for localization of bcd RNA. At stage 10b, we find gammaTub37C and Dgrip75 anteriorly concentrated and propose the formation of a microtubule-organizing center at the anterior pole of the oocyte.
authors.affiliation: Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstr. 35, 72076, Tübingen, Germany. frank.schnorrer@tuebingen.mpg.de
authors.forename: Frank
authors.initials: F
authors.lastname: Schnorrer
authors.forename: Stefan
authors.initials: S
authors.lastname: Luschnig
authors.forename: Iris
authors.initials: I
authors.lastname: Koch
authors.forename: Christiane
authors.initials: C
authors.lastname: Nüsslein-Volhard
mesh_terms: D000818:Animals; D018385:Centrosome / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D005260:Female; D017344:Genes, Insect; D049452:Green Fluorescent Proteins; D018398:Homeodomain Proteins / Q000235:genetics*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D022101:Microtubule-Organizing Center / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D016296:Mutagenesis; D009865:Oocytes / Q000378:metabolism; D009866:Oogenesis / Q000502:physiology*; D012313:RNA / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics*; D014404:Tubulin / Q000235:genetics / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; C469730:Grip75 protein, Drosophila; D018398:Homeodomain Proteins; D008164:Luminescent Proteins; D008869:Microtubule-Associated Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014404:Tubulin; C056478:bcd protein, Drosophila; C469729:gammaTub37C protein, Drosophila; C073970:swa protein, Drosophila; D049452:Green Fluorescent Proteins; D012313:RNA