Submerged and solid-state production of laccase and Mn-peroxidase by Panus tigrinus on olive mill wastewater-based media.
The possible use of olive-mill wastewater (OMW) as a growth medium for the production of extracellular laccase and manganese peroxidase (MnP) from the white-rot fungus Panus tigrinus (P. tigrinus) CBS 577.79 was studied using a properly formulated OMW-based medium (2-fold diluted OMW supplemented with 0.5% sucrose and 0.1% yeast extract) either in a stirred-tank or an air-lift reactor. Solid-state fermentation (SSF) was also performed in a rotary drum reactor using maize stalks moistened with the OMW-based medium. Highest levels of laccase and manganese peroxidase activity were obtained in the stirred-tank reactor (4600+/-98 U l(-1) on day 13) and in the air-lift reactor (410+/-22 on day 7), respectively. Based on total enzyme activities, SSF appears to be more suitable than LSF but the latter exhibits better volumetric productivities.
Fenice
Massimiliano
M
Dipartimento di Agrobiologia e Agrochimica, University of Tuscia, Via San Camillo de Lellis snc, 01100 Viterbo, Italy.
Giovannozzi Sermanni
Giovanni
G
Federici
Federico
F
D'Annibale
Alessandro
A
D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development; D001673:Biodegradation, Environmental; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D007220:Industrial Waste / Q000517:prevention & control; D042845:Laccase; D000069463:Olive Oil; D010088:Oxidoreductases / Q000096:biosynthesis*; D010544:Peroxidases / Q000096:biosynthesis*; D010636:Phenols / Q000378:metabolism; D010938:Plant Oils; D012996:Solutions; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000378:metabolism
D007220:Industrial Waste; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; D012996:Solutions; D014874:Water Pollutants, Chemical; D010088:Oxidoreductases; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase