Competitive hybridization on spotted microarrays as a tool to conduct comparative genomic analyses of Xylella fastidiosa strains.
Xylella fastidiosa strains are responsible for several plant diseases and since such isolates display a broad host range and complex biological behavior, genomic comparisons employing microarray hybridizations may provide an effective method to compare them. Thus, we performed a thorough validation of this type of approach using two recently sequenced strains of this phytopathogen. By matching microarray hybridization results to direct sequence comparisons, we were able to establish precise cutoff ratios for common and exclusive sequences, allowing the identification of exclusive genes involved in important biological traits. This validation will enable the use of microarray-based comparisons across a wide variety of microorganisms
Costa de Oliveira
Regina
R
Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, SP, Brazil. reginaco@umc.br
Yanai
Giane M
GM
Muto
Nair H
NH
Leite
Daniela B
DB
de Souza
Alessandra A
AA
Coletta Filho
Helvécio D
HD
Machado
Marcos A
MA
Nunes
Luiz R
LR
D016680:Genome, Bacterial; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D010935:Plant Diseases / Q000382:microbiology*; D011548:Pseudomonadaceae / Q000235:genetics*; D017385:Sequence Homology