Treatment with prostaglandin F2alpha increases expression of prostaglandin synthase-2 in the rat corpus luteum.
Recent studies indicate that the corpus luteum (CL) may be a source of prostaglandin F2alpha (PGF2alpha) for regression. We investigated expression of mRNA and protein for prostaglandin G/H synthase (PGHS) in the CL of immature superovulated rats following administration of PGF2alpha. We observed an increase in mRNA for PGHS-2, the induced isoform, at 1 h and protein at 8 and 24 h after treatment. One hour after PGF2alpha, there was also a progressive decrease in plasma progesterone concentration. There were no changes, however, in expression of PGHS-1, the constitutive isoform, over the 24 h sampling period. These results indicate that PGHS-2 increases following PGF2alpha treatment and that expression of this enzyme in the rat CL may contribute to the luteolytic mechanism.
Narayansingh
R M
RM
Department of Biology, University of Waterloo, Ont., Canada.
Senchyna
M
M
Carlson
J C
JC
D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D015237:Dinoprost / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression; D003341:Luteolysis / Q000187:drug effects*; D011374:Progesterone / Q000097:blood; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013480:Superovulation / Q000097:blood
D011374:Progesterone; D015237:Dinoprost; D011451:Prostaglandin-Endoperoxide Synthases