Effect of 4-hydroxynonenal, a lipid peroxidation product, on exocytosis in HL-60 cells.
Our work analysed the effect of 4-hydroxynonenal (HNE), a chemotactic aldehydic end-product of lipid peroxidation, on exocytosis in HL-60 cells. We measured the release of beta-glucuronidase, an enzyme of azurophil granules, from the cells incubated at 37 degrees C for 10 min in the presence of HNE concentrations ranging between 10(-8) and 10(-5) M. The release of lactate dehydrogenase was assayed to test cell viability. HNE (1 microM) was able to induce a significant and strong stimulation of beta-glucuronidase secretion without leading to cytotoxic effects. The finding that HNE could increase the exocytotic secretion from HL-60 cells together with its known chemotactic property supports the hypothesis that this lipid peroxidation product may play an important role as a chemical mediator of inflammation; moreover it is noteworthy that micromolar concentrations of HNE have actually been found in exudates from acute and chronic inflammations.
Maggiora
Marina
M
Department of Experimental Medicine and Oncology, Section of General Pathology, University of Turin, Corso Raffaello 30, 10125 Turin, Italy.
Dianzani
Mario Umberto
MU
Rossi
Maria Armida
MA
D000447:Aldehydes / Q000494:pharmacology*; D002470:Cell Survival; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005089:Exocytosis; D005966:Glucuronidase / Q000378:metabolism; D006131:Growth Inhibitors / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D007249:Inflammation; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation; D013696:Temperature; D013997:Time Factors
D000447:Aldehydes; D015853:Cysteine Proteinase Inhibitors; D006131:Growth Inhibitors; D007770:L-Lactate Dehydrogenase; D005966:Glucuronidase; C027576:4-hydroxy-2-nonenal