Overexpression of cyclin A in hepatocellular carcinoma and its relationship with HBx gene integration.
OBJECTIVE
To investigate the frequency of cyclin A overexpression in hepatocellular carcinoma (HCC) and its relationship with clinical significance and HBx gene integration.


METHODS
PCR, RT-PCR and Western blot were used to detect the gene, mRNA and protein level of cyclin A in the tumor and nontumorous tissue. PCR and Southern blot were used to detect the integration of HBx gene in HCC.


RESULTS
Amplification of cyclin A gene was found in 1 of 35 patients; overexpression of cyclin A mRNA and protein was found in 16 of 35, 21 of 35 patients, respectively. Overexpression of cyclin A protein was correlated with patient's age, tumor size and HBx integration.


CONCLUSIONS
Overexpression of cyclin A occurs in the early stage of HCC carcinogenesis. It may be one of the important approaches by which HBV affects the normal cell cycle of hepatocyte.
Zhang
Yi
Y
Department of General Surgery, First People's Hospital, Shanghai 200080, China.
Peng
Zhihai
Z
Qiu
Guoqiang
G
Wang
Zhaowen
Z
Gu
Wei
W
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology / Q000821:virology; D019925:Cyclin A / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger; D015534:Trans-Activators / Q000235:genetics*; D054334:Viral Regulatory and Accessory Proteins; D016662:Virus Integration
D019925:Cyclin A; D012333:RNA, Messenger; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein