Visualizing the structure and mechanism of a small nucleolytic ribozyme.
Time-resolved crystallography has recently evolved into a powerful and invaluable technique for observing conformational and chemical intermediate states at or near atomic resolution in protein enzymes. The application of monochromatic time-resolved X-ray crystallographic freeze-trapping experiments to an RNA enzyme, the hammerhead ribozyme, is outlined here.
Scott
William G
WG
Department of Chemistry and Biochemistry, Center for Molecular Biology of RNA, University of California, Santa Cruz, CA 95064, USA. wgscott@chemistry.ucsc.edu
D003461:Crystallography; D007700:Kinetics; D011487:Protein Conformation; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D012107:Research Design
D016337:RNA, Catalytic