Urinary steroids in men with male-pattern alopecia.
Enzyme hydrolysis, solid phase extraction, methoxym-silyl derivatization and capillary gas chromatographic analysis were used to examine the changes in urinary steroid metabolites in men with androgenic alopecia. A total of 23 men with androgenic alopecia and 7 age-matched control healthy men collected 24-h urine. Significantly increased values were found in the metabolites of testosterone (T): androsterone (A) (p<0.02), and etiocholanolone (E) (p<0.05) in patients with androgenic alopecia, compared to the control values. Elevated levels of 16-hydroxy-dehydroepiandrosterone (16-OHD) (p<0.03) and cortisol (F) (P<0.05) were found, but the levels of cortisol metabolites were unchanged. Calculating the ratio of total 5 alpha/5 beta metabolites provided information on the activity of 5 alpha-reductase. The ratio of total 5 alpha/5 beta metabolites was increased in the patients showing the increased 5 alpha-reductase activity. The elevated 16-OHD level could be indicative of patients who had mild hyperadrenal activity.
Poór
V
V
Central Research Laboratory, Faculty of Medicine, Pécs University Medical School, 12Szigeti út, 1Honvéd u. H-7643 Pécs, Hungary. Viktoria.Poor@AOK.PTE.hu
Juricskay
S
S
Telegdy
E
E
D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000652:urine; D000328:Adult; D000505:Alopecia / Q000652:urine*; D000738:Androsterone / Q000652:urine; D002849:Chromatography, Gas; D003687:Dehydroepiandrosterone / Q000652:urine; D005043:Etiocholanolone / Q000652:urine; D006801:Humans; D006854:Hydrocortisone / Q000652:urine; D008297:Male; D012016:Reference Values; D013256:Steroids / Q000652:urine*; D013739:Testosterone / Q000652:urine
D013256:Steroids; D013739:Testosterone; D003687:Dehydroepiandrosterone; D005043:Etiocholanolone; D000738:Androsterone; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase; D006854:Hydrocortisone