Effect of E1B mutant adenovirus on p53-deficient leukemic cells.
OBJECTIVE: To study the effect of E1B mutant adenovirus (dl1520) on 3 p53-deficient leukemic cell lines K562, Jurkat and HL-60. METHODS: The replication efficiency of dl1520 in the 3 leukemic cell lines was assessed by plaque assay, and the number of cells killed by the adenovirus determined by using trypan-blue in a course of 10 d following the infection. RESULTS: The replication efficiency of dl1520 in the 3 leukemic cell lines was significantly lower than that in the positive control, and no significant cytocidal effect against the 3 cell lines was observed. CONCLUSION: dl1520 can not inhibit the malignant blood cells as K562, Jurkat and HL-60 cell lines.
Rao
Jin-Jun
JJ
Institute of Pharmaceutic Science, First Military Medical University, Guangzhou 510515, China.
Zhu
Zheng-Guang
ZG
Xu
Wei
W
Wu
Shu-Guang
SG