Structural determination of hexadecanoic lysophosphatidylcholine regioisomers by fast atom bombardment tandem mass spectrometry.
The structural determination of sn-1 and sn-2 hexadecanoic lysophosphatidylcholine (LPC) regioisomers was carried out using fast atom bombardment tandem mass spectrometry (FAB-MS/MS). The collision-induced dissociation (CID) of protonated and sodiated molecules produced diverse product ions due mainly to charge remote fragmentations. Based on the information obtained from the CID spectra of protonated and sodiated molecules, sn-1 and sn-2 hexadecanoic LPC isomers could be discriminated. Especially, the abundance ratio of the diagnostic ion pair [m/z 224/226] in the CID spectra of [M + H](+) ions was shown to be greatly different. Moreover, the CID-MS/MS spectra of sodium-adducted molecules for hexadecanoic LPC isomers showed characteristic product ions such as [M + Na - 103](+), [M + Na - 85](+), and [M + Na - 59](+), by which their regio-specificity can be differentiated.
Hong
Jongki
J
Hazardous Substance Research Team, Korea Basic Science Institute, Seoul 136-701, Korea. jongki@kbsi.re.kr
Kim
Young Hwan
YH
Gil
Ji Hye
JH
Cho
Kun
K
Jung
Jee H
JH
Han
So-Yeop
SY
D008244:Lysophosphatidylcholines / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods*
C470482:1-hydroxy-2-hexadecanoyl-sn-glycero-3-phosphocholine; D008244:Lysophosphatidylcholines