title: Clinical and experimental study of two cases of myelodysplastic syndrome with t(3; 5) (q25; q34) translocation.
abstract: OBJECTIVE
  To report two myelodysplatic syndromes (MDS) patients with t(3; 5) (q25; q34).
  
  
  METHODS
  Chromosome specimens were prepared by short-term culture of bone marrow cells. Karyotype analysis was performed by R banding technique, chromosome painting (fluorescence in situ hybridization, FISH) by using whole chromosome 3 and 5 probes in case 1.
  
  
  RESULTS
  The clinical and hematological findings were compatible with diagnosis of MDS. Karyotype analysis showed that both patients had identical t(3; 5) (q25; q34) translocation. A reciprocal translocation between chromosomes 3q and 5q was proved by FISH in one patient.
  
  
  CONCLUSIONS
  t(3; 5) translocation is a rare chromosome abnormality specifically associated with MDS and frequently displays trilineage dysplasia. Chromosome painting technique is a reliable tool for detecting this translocation.
authors.affiliation: The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Suzhou 215006, China.
authors.forename: Yafang
authors.initials: Y
authors.lastname: Wu
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Xue
authors.forename: Meng
authors.initials: M
authors.lastname: Zhao
authors.forename: Saijuan
authors.initials: S
authors.lastname: Chen
authors.forename: Jinlan
authors.initials: J
authors.lastname: Pan
authors.forename: Dingwei
authors.initials: D
authors.lastname: Lu
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D002893:Chromosomes, Human, Pair 3; D002895:Chromosomes, Human, Pair 5; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007963:Leukocytes, Mononuclear / Q000145:classification / Q000276:immunology; D008297:Male; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000503:physiopathology; D014178:Translocation, Genetic
chemical_list: D015703:Antigens, CD