Significant differences in capillary electrophoretic patterns of follicular fluids and sera from women pre-treated for in vitro fertilization.
Human ovarian follicular fluids and sera obtained from women pre-treated for in vitro fertilization (IVF) were investigated by capillary zone electrophoresis. Comparison of the matching physiological liquids showed substantial differences in the electrophoretic patterns. Significant decrease in the alpha(1)- and gamma-fractions of follicular fluids of every woman were observed, whereas other fractions of the samples did not show such alterations. Since follicular fluid is a product of both, secretion by granulosa cells and diffusion from the theca capillaries, we can assume that the forced production of follicular fluid upon hormone stimulation (with gonadotropin releasing hormone (GnRH), follicle stimulating hormone (FSH) and corionic gonadotroph hormone (hCG)) may play role in the uneven presence of the proteins.
Rezeli
Melinda
M
Department of Analytical Chemistry, Faculty of Sciences, University of Pécs, Ifjúság útja 6., 7624 Pécs, Hungary.
Világhy
Bernadett
B
Kilár
Ferenc
F
Kanyó
Katalin
K
Török
Béla
B
Török
Attila
A
D001797:Blood Protein Electrophoresis / Q000379:methods; D001798:Blood Proteins / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D015571:Follicular Fluid / Q000737:chemistry*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000188:drug therapy / Q000378:metabolism / Q000628:therapy*; D010062:Ovulation Induction / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis; D012712:Serum Globulins / Q000032:analysis; D013480:Superovulation / Q000187:drug effects
D001798:Blood Proteins; D006454:Hemoglobins; D012709:Serum Albumin; D012712:Serum Globulins; D007987:Gonadotropin-Releasing Hormone; D005640:Follicle Stimulating Hormone