Isolation and characterization of bile salts-sensitive mutants of Enterococcus faecalis.
A library of insertional mutants of Enterococcus faecalis was constructed; it allowed the isolation and the characterization of 10 mutants affected in resistance to bile salts. Insertion loci of two mutants corresponded to genes of unknown function, while the amino acid sequences deduced from the other loci were homologous to proteins related to DNA repair, oxidative response, transcriptional regulation, dGTP hydrolysis, membrane composition, or cell wall synthesis. Further characterization of one mutant revealed that the insertion within the E. faecalis sagA gene led to a decrease of the resistance towards numerous independent physicochemical stresses, to modifications of the cell wall integrity, and to perturbations of cell division with septation anomalies.
Breton
Yoann Le
YL
Laboratoire de Microbiologie de l'Environnement, EA 956 USC INRA, Institut de Recherche en Biologie Appliquée, Université de Caen, 14032 Caen Cedex, France.
Mazé
Alain
A
Hartke
Axel
A
Lemarinier
Sylviane
S
Auffray
Yanick
Y
Rincé
Alain
A
D001426:Bacterial Proteins / Q000235:genetics; D001647:Bile Acids and Salts / Q000494:pharmacology*; D003470:Culture Media; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D008855:Microscopy, Electron, Scanning; D016254:Mutagenesis, Insertional; D009154:Mutation
D001426:Bacterial Proteins; D001647:Bile Acids and Salts; D003470:Culture Media