title: Isolation of bovine viral diarrhea virus from an alpaca.
abstract: A frozen, stillborn alpaca (Lama pacos) was submitted to the Minnesota Veterinary Diagnostic Laboratory for diagnostic purposes. No gross or histopathologic changes of any significance were seen. A pool of lung, liver, and brain tissues was positive for bovine viral diarrhea virus (BVDV) by reverse transcription-polymerase chain reaction. On inoculation in cell cultures, a noncytopathic BVDV (type 1b) was isolated. No evidence of BVDV was seen on immunohistochemical examination of tissues. This indicates the importance of using multiple tests for arriving at a diagnosis and appears to be the first report of BVDV isolation from alpaca.
authors.affiliation: Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
authors.forename: Sagar M
authors.initials: SM
authors.lastname: Goyal
authors.forename: Mostafa
authors.initials: M
authors.lastname: Bouljihad
authors.forename: Sigrun
authors.initials: S
authors.lastname: Haugerud
authors.forename: Julia F
authors.initials: JF
authors.lastname: Ridpath
mesh_terms: D000818:Animals; D002161:Camelids, New World / Q000821:virology*; D001908:Diarrhea Viruses, Bovine Viral / Q000302:isolation & purification* / Q000472:pathogenicity; D005260:Female; D005313:Fetal Death / Q000662:veterinary; D007150:Immunohistochemistry; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction