Ursolic acid as a trypanocidal constituent in rosemary.
The MeOH extract of the leaves of rosemary (Rosmarinus officinalis L.) completely inhibited the motility of cultured epimastigotes of Trypanosoma cruzi at the concentration of 2 mg/ml after 2 h of incubation. Activity-guided fractionation of the MeOH extract has resulted in the isolation of three triterpene acids, betulinic, oleanolic and ursolic acids. Ursolic acid stopped the movement of all T. cruzi epimastigotes at the minimum concentration (MC(100)) of 40 micro g/ml (88 micro M) after 48 h of incubation. Oleanolic acid was less active (MC(100): 250 micro g/ml, 550 micro M) and betulinic acid was practically inactive.
Abe
Fumiko
F
Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
Yamauchi
Tatsuo
T
Nagao
Tsuneatsu
T
Kinjo
Junei
J
Okabe
Hikaru
H
Higo
Hiroo
H
Akahane
Hiroshige
H
D000818:Animals; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D027542:Rosmarinus / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D014344:Trypanocidal Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D000097245:Ursolic Acid
D010936:Plant Extracts; D014315:Triterpenes; D014344:Trypanocidal Agents; D000097245:Ursolic Acid