title: [Diffuse lamellar keratitis: prophylactic treatment with ketorolac tromethamine 0.5% in an animal model].
abstract: OBJECTIVE
  The objective of this study is to evaluate the use of a topical non-steroidal anti-inflammatory drug in the treatment of induced diffuse lamellar keratitis (DLK) in an animal model.
  
  
  METHODS
  A corneal flap was created in 40 eyes of 20 Dutch-belted rabbits using the ASC microkeratome. The interface was inoculated with either Pseudomonas Aeruginosa Lipopolysaccharide (LPS) endotoxin or Ultra Palmolive liquid dish washer. The rabbits were divided in two groups: Group I (n=20) treated with ketorolac tromethamine ophthalmic solution 0.5% 4 times a day and the group II (n=20) used as control. The rabbits were examined at the slit lamp at day 1, 3, 5 and 7 postoperatively. DLK was graded from I-IV.
  
  
  RESULTS
  At the end of the study 31 eyes were available for evaluation. 28 eyes (90%) developed DLK: 86% of the treated group and 94% of the control group during the follow-up. The treated group showed a lower rate of DLK as well as a lower severity. However, no statistically significant difference was found when comparing both groups (P>0.05).
  
  
  CONCLUSIONS
  Pseudomonas aeruginosa LPS endotoxin and Palmolive Ultra can induce DLK in rabbit eyes. The postoperative prophylactic treatment with a topical non-steroidal anti-inflammatory drug showed a tendency towards a lower DLK rate as well as the severity of the disease.
authors.affiliation: Magill Research Center for Vision Correction, Storm Eye Institute, Medical University of South Carolina, SC 29425, USA. sandoval@musc.edu
authors.forename: H P
authors.initials: HP
authors.lastname: Sandoval
authors.forename: L G
authors.initials: LG
authors.lastname: Vargas
authors.forename: M P
authors.initials: MP
authors.lastname: Holzer
authors.forename: T J
authors.initials: TJ
authors.lastname: Kasper
authors.forename: D T
authors.initials: DT
authors.lastname: Vroman
authors.forename: D J
authors.initials: DJ
authors.lastname: Apple
authors.forename: K D
authors.initials: KD
authors.lastname: Solomon
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D007634:Keratitis / Q000188:drug therapy* / Q000517:prevention & control; D020731:Keratomileusis, Laser In Situ; D020911:Ketorolac Tromethamine / Q000627:therapeutic use*; D023421:Models, Animal; D009883:Ophthalmic Solutions; D011817:Rabbits; D013524:Surgical Flaps
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D009883:Ophthalmic Solutions; D020911:Ketorolac Tromethamine