title: Predictive value of circulating immature cell counts in peripheral blood for timing of peripheral blood progenitor cell collection after G-CSF plus chemotherapy-induced mobilization.
abstract: BACKGROUND
  Enumeration of CD34+ cells in peripheral blood (PB) before apheresis predicts the number of CD34+ cells collected, although flow cytometric techniques used are complex and expensive. In an attempt to determine the optimal timing for peripheral blood progenitor cell (PBPC) collection, the usefulness of circulating immature cell (CIC) counts in PB was evaluated.
  
  
  METHODS
  CIC counts in PB and CD34+ cell counts in the apheresis product from 249 collections were assessed, and the relationship between these two parameters was evaluated by with the Pearson rank correlation analysis, the Fisher exact test, and the U-test.
  
  
  RESULTS
  CIC counts were correlated significantly with the number of CD34+ cells per kg of patient's body weight in the apheresis product (Pearson rank correlation analysis: r = 0.635, p < 0.0001). When a level of 1 x 10(9) CICs per L was selected as a cutoff value, the sensitivity and specificity for collecting more than 1 x 10(6) CD34+ cells per kg of body weight were 75.7 and 85.5 percent, respectively.
  
  
  CONCLUSIONS
  The present study strongly suggests that the number of CICs in PB may estimate the number of CD34+ cells collected. The data indicate that CIC counts above 1 x 10(9) per L can be used as a good predictor for PBPC collections containing more than 1 x 10(6) CD34+ cells per kg of body weight in a single apheresis procedure.
authors.affiliation: Second Department of Internal Medicine, Okayama University Medical School, Okayama, Japan. kozuka@md.okayama-u.ac.jp
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Kozuka
authors.forename: Kazuma
authors.initials: K
authors.lastname: Ikeda
authors.forename: Takanori
authors.initials: T
authors.lastname: Teshima
authors.forename: Kensuke
authors.initials: K
authors.lastname: Kojima
authors.forename: Keitaro
authors.initials: K
authors.lastname: Matsuo
authors.forename: Akihiro
authors.initials: A
authors.lastname: Bessho
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Sunami
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hiramatsu
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Maeda
authors.forename: Toshio
authors.initials: T
authors.lastname: Noguchi
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Nobuharu
authors.initials: N
authors.lastname: Fujii
authors.forename: Toshi
authors.initials: T
authors.lastname: Imai
authors.forename: Katsuto
authors.initials: K
authors.lastname: Takenaka
authors.forename: Katsuji
authors.initials: K
authors.lastname: Shinagawa
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Ishimaru
authors.forename: Kenji
authors.initials: K
authors.lastname: Niiya
authors.forename: Norio
authors.initials: N
authors.lastname: Koide
authors.forename: Mitsune
authors.initials: M
authors.lastname: Tanimoto
authors.forename: Mine
authors.initials: M
authors.lastname: Harada
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D001772:Blood Cell Count; D016922:Cellular Senescence; D003131:Combined Modality Therapy; D003561:Cytarabine / Q000008:administration & dosage / Q000494:pharmacology; D003907:Dexamethasone / Q000008:administration & dosage / Q000494:pharmacology; D005047:Etoposide / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007069:Ifosfamide / Q000008:administration & dosage / Q000494:pharmacology; D007937:Leukapheresis; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood / Q000188:drug therapy / Q000628:therapy; D036102:Peripheral Blood Stem Cell Transplantation; D011237:Predictive Value of Tests; D012372:ROC Curve; D014182:Transplantation, Autologous
chemical_list: D018952:Antigens, CD34; D003561:Cytarabine; D016179:Granulocyte Colony-Stimulating Factor; D005047:Etoposide; D003907:Dexamethasone; D007069:Ifosfamide