Comparative analysis of the outer membrane protein profiles of isolates of the Pasteurella multocida (B:2) associated with haemorrhagic septicaemia.
Outer membrane proteins (OMP) of P. multocida (serotype B:2) field isolates (n = 6) and a vaccine strain (P-52) were extracted by a sarkosyl method and characterized using SDS-PAGE and immunoblotting. About 20 polypeptide bands were observed in the profile of the vaccine strain with MW ranging from 16 to 90 kDa and, based on band thickness and intensity of staining, three polypeptides of MW 31, 33 and 37 kDa were considered to be the major OMPs. The profiles of the field isolates showed minor differences when compared with that of the vaccine strain. The OMP of 33 kDa was only expressed by the vaccine strain. Four field isolates expressed an OMP of 39 kDa, which did not appear in the profiles of the remaining two field isolates and the P-52 strain. Similarly, an OMP of 25 kDa was exclusively seen in the profile of a single isolate. By immunoblotting studies, using anti-P. multocida (P-52) whole-cell hyperimmune serum raised in rabbits as well as buffalo immune sera, it became evident that the polypeptide of 37 kDa was the most antigenic OMP in the profiles of all the isolates, including the P-52 strain. Other polypeptides were either weakly antigenic or visible in the profile of only a few of the isolates. The study thus identified the major OMP of P. multocida (B:2) and suggested that this highly antigenic 37 kDa OMP has potential for further protective and immunodiagnostic studies.
Tomer
P
P
Department of Veterinary Microbiology, College of Veterinary Sciences, CCS Haryana Agricultural University, Hisar, India. tomerpankaj@yahoo.com
Chaturvedi
G C
GC
Minakshi
Malik
P
P
Monga
D P
DP
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology; D002020:Buffaloes / Q000382:microbiology; D002417:Cattle / Q000382:microbiology; D004196:Disease Outbreaks; D004591:Electrophoresis, Polyacrylamide Gel; D006483:Hemorrhagic Septicemia / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology* / Q000662:veterinary*; D015151:Immunoblotting; D007194:India / Q000453:epidemiology; D016979:Pasteurella multocida / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines