title: Residues of 14C-4n-nonylphenol in mosquitofish (Gambusia holbrooki) oocytes and embryos during dietary exposure of mature females to this xenohormone.
abstract: In order to assess in fish the maternal transfer of alkylphenolic compounds to the progeny, the identification and quantification of the labelled compounds present in oocytes and embryos was conducted after dietary exposure of mature female mosquitofish to 14C-4n-nonylphenol during vitellogenesis and embryogenesis respectively. Radioactivity found in bile and liver extracts accounted for 0.9-0.6 and 0.2-0.1% of ingested radioactivity for females exposed during vitellogenesis and embryogenesis respectively. The amount of extractable radioactivity present in oocytes and embryos was 0.19 and 0.07% of the ingested dose respectively. The radio-HPLC profiles obtained from bile, liver, oocyte and embryo extracts were similar. They showed the presence of 4n-NP-glucuronide as the major metabolite and traces of unchanged 4n-NP. The other metabolites corresponded to 8-hydroxynonylphenol, 9-(4-hydroxyphenyl)-nonanoic acid and para-hydroxybenzoic acid which is the final product of the alkyl chain oxidation. Our results indicate that exposure of ovoviviparous female fish to 4-NP during vitellogenesis and embryogenesis leads to the contamination of the progeny by 4-NP and its metabolites.
authors.affiliation: UMR 1089 Xénobiotiques, INRA/ENSAT/ENVT, Toulouse, France.
authors.forename: Remi
authors.initials: R
authors.lastname: Thibaut
authors.forename: Gilles
authors.initials: G
authors.lastname: Monod
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Cravedi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D003532:Cyprinodontiformes / Q000196:embryology*; D004032:Diet; D004625:Embryo, Nonmammalian / Q000737:chemistry; D005314:Embryonic and Fetal Development; D004703:Endocrine System / Q000187:drug effects; D005260:Female; D018811:Maternal Exposure; D009865:Oocytes / Q000737:chemistry; D010636:Phenols / Q000008:administration & dosage / Q000493:pharmacokinetics*; D014818:Vitellogenesis; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000493:pharmacokinetics*
chemical_list: D002250:Carbon Radioisotopes; D010636:Phenols; D014874:Water Pollutants, Chemical; C025256:nonylphenol