title: DNA Pooling: a tool for large-scale association studies.
abstract: DNA pooling is a practical way to reduce the cost of large-scale association studies to identify susceptibility loci for common diseases. Pooling allows allele frequencies in groups of individuals to be measured using far fewer PCR reactions and genotyping assays than are used when genotyping individuals. Here, we discuss recent developments in quantitative genotyping assays and in the design and analysis of pooling studies. Sophisticated pooling designs are being developed that can take account of hidden population stratification, confounders and inter-loci interactions, and that allow the analysis of haplotypes.
authors.affiliation: P080, Institute of Psychiatry, King's College, Denmark Hill, London SE5 8AF, UK. p.sham@iop.kcl.ac.uk
authors.forename: Pak
authors.initials: P
authors.lastname: Sham
authors.forename: Joel S
authors.initials: JS
authors.lastname: Bader
authors.forename: Ian
authors.initials: I
authors.lastname: Craig
authors.forename: Michael
authors.initials: M
authors.lastname: O'Donovan
authors.forename: Michael
authors.initials: M
authors.lastname: Owen
mesh_terms: D000818:Animals; D004247:DNA; D005787:Gene Frequency; D005821:Genetic Techniques; D006239:Haplotypes; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: D004247:DNA