title: Influence of calcium hydroxide root-canal sealer on microbial growth in vitro.
abstract: The calcium hydroxide-based filling material Apexit, which is often used in endodontic practice, was evaluated for its antibacterial and antifungal effects against microorganisms isolated from oral cavity (Serratia marcescens, Pseudomonas putida, Staphylococcus aureus, Candida albicans). Two different quantitative techniques were employed--the direct-contact test was used to examine the efficacy of freshly mixed material while the broth-survival test was employed to check the antimicrobial properties of 5-d-old material. Apexit inhibited Gram-negative bacteria more effectively than Gram-positive ones but had none or a very weak inhibitory effect on C. albicans.
authors.affiliation: Department of Oral Pathology, Department of Stomatology, Medical Faculty, University of Rijeka, Croatia. sonja.pezelj-ribaric@ri.hinet.hr
authors.forename: S
authors.initials: S
authors.lastname: Pezelj-Ribarić
authors.forename: I
authors.initials: I
authors.lastname: Brekalo
authors.forename: M
authors.initials: M
authors.lastname: Abram
authors.forename: M
authors.initials: M
authors.lastname: Dorić
authors.forename: I
authors.initials: I
authors.lastname: Miletić
authors.forename: Z
authors.initials: Z
authors.lastname: Karlović
mesh_terms: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D066298:In Vitro Techniques; D009055:Mouth / Q000382:microbiology; D016958:Pseudomonas putida / Q000187:drug effects / Q000254:growth & development; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012390:Root Canal Therapy; D012706:Serratia marcescens / Q000187:drug effects / Q000254:growth & development; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; C074418:Apexit; D012387:Root Canal Filling Materials; D002126:Calcium Hydroxide