[Expression of core-binding factor a1 by human skin fibroblasts induced in vitro].
OBJECTIVE
To investigate the probabilities of core-biding factor a1 (Cbfa1) expression by human skin fibroblasts induced in vitro.


METHODS
The fibroblasts were isolated, purified from human skin, and were grown in incubation in the media of TNF-alpha, BMP-2, and combined TNF-alpha and BMP-2 at certain concentrations, respectively. The changes in biological features of these fibroblasts correlated with osteogenesis were detected by immunohistochemistry and RT-PCR assay.


RESULTS
TNF-alpha could switch phenotype of collagen in fibroblasts from Type I and III to Type I and induce fibroblasts to express Ras and BMP type I receptor (BMPR-IA). TNF-alpha in combination with BMP-2 could induce fibroblasts to express Cbfa1 and osteocalcin mRNA.


CONCLUSIONS
Human skin fibroblast could be induced into pro-osteoblast expressing Cbfa1, an osteoblast-specific transcription factor and a regulation of osteoblast differentiation, and combined use of TNF-alpha and BMP-2 was one of the regulating factors.
Deng
Lianfu
L
Shanghai Institute of Traumatology and Orthopaedics, Shanghai 200025, China.
Feng
Wei
W
Zhang
Yue
Y
Zhu
Yaping
Y
D055396:Bone Morphogenetic Protein 2; D052005:Bone Morphogenetic Protein Receptors, Type I; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D050659:Core Binding Factor Alpha 1 Subunit; D050660:Core Binding Factors; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D009363:Neoplasm Proteins; D015675:Osteocalcin / Q000096:biosynthesis; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D017978:Receptors, Growth Factor / Q000096:biosynthesis; D012867:Skin / Q000166:cytology; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D050659:Core Binding Factor Alpha 1 Subunit; D050660:Core Binding Factors; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D017978:Receptors, Growth Factor; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D015675:Osteocalcin; D003094:Collagen; D017346:Protein Serine-Threonine Kinases; C498211:BMPR1A protein, human; D052005:Bone Morphogenetic Protein Receptors, Type I