Development of a hexaplex PCR assay for rapid detection of virulence and regulatory genes in Vibrio cholerae and Vibrio mimicus.
We have developed a hexaplex PCR assay for rapid detection of the virulence and regulatory genes for cholera toxin enzymatic subunit A (ctxA), zonula occludens toxin (zot), accessory cholera enterotoxin (ace), toxin-coregulated pilus (tcpA), outer membrane protein (ompU), and central regulatory protein ToxR (toxR) in Vibrio cholerae and Vibrio mimicus. This hexaplex PCR proved successful in screening pathogenic-toxigenic and nonpathogenic-nontoxigenic V. cholerae and V. mimicus strains from both clinical and environmental sources.
Singh
D V
DV
Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram 695 014, Kerala, India. durg-singh@mailcity.com
Isac
Sree Renjini
SR
Colwell
R R
RR
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002771:Cholera / Q000821:virology; D002772:Cholera Toxin / Q000235:genetics / Q000378:metabolism; D004783:Environmental Microbiology; D005809:Genes, Regulator; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors; D014733:Vibrio / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D014734:Vibrio cholerae / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D002772:Cholera Toxin