title: A genomic switch at the transition from cell proliferation to terminal differentiation in the Drosophila eye.
abstract: Organogenesis involves cell proliferation followed by complex determination and differentiation events that are intricately controlled in time and space. The instructions for these different steps are, to a large degree, implicit in the gene expression profiles of the cells that partake in organogenesis. Combining fluorescence-activated cell sorting and SAGE, we analyzed genomic expression patterns in the developing eye of Drosophila melanogaster. Genomic activity changes as cells pass from an uncommitted proliferating progenitor state through determination and differentiation steps toward a specialized cell fate. Analysis of the upstream sequences of genes specifically expressed during the proliferation phase of eye development implicates the transcription factor DREF and its inhibitor dMLF in the control of cell growth in this organ.
authors.affiliation: Department of Biomedical Genetics, University of Rochester Medical Center, Rochester, NY 14642, USA. henri_jasper@urmc.rochester.edu
authors.forename: Heinrich
authors.initials: H
authors.lastname: Jasper
authors.forename: Vladimir
authors.initials: V
authors.lastname: Benes
authors.forename: Ann
authors.initials: A
authors.lastname: Atzberger
authors.forename: Silvia
authors.initials: S
authors.lastname: Sauer
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Ansorge
authors.forename: Dirk
authors.initials: D
authors.lastname: Bohmann
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics; D005123:Eye / Q000166:cytology / Q000196:embryology*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D038081:Organogenesis / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*
chemical_list: C099494:Dref protein, Drosophila; D029721:Drosophila Proteins; C468117:Mlf protein, Drosophila; D014157:Transcription Factors