The pore-forming domain of colicin A fused to a signal peptide: a tool for studying pore-formation and inhibition.
Pore-forming colicins are plasmid-encoded bacteriocins that kill Escherichia coli and closely related bacteria. They bind to receptors in the outer membrane and are translocated across the cell envelope to the inner membrane where they form voltage-dependent ion-channels. Colicins are composed of three domains, with the C-terminal domain responsible for pore-formation. Isolated C-terminal pore-forming domains produced in the cytoplasm of E. coli are inactive due to the polarity of the transmembrane electrochemical potential, which is the opposite of that required. However, the pore-forming domain of colicin A (pfColA) fused to a prokaryotic signal peptide (sp-pfColA) is transported across and inserts into the inner membrane of E. coli from the periplasmic side, forming a functional channel. Sp-pfColA is specifically inhibited by the colicin A immunity protein (Cai). This construct has been used to investigate colicin A channel formation in vivo and to characterise the interaction of pfColA with Cai within the inner membrane. These points will be developed further in this review.
Duché
Denis
D
Laboratoire d'Ingéniérie des Systèmes Macromoléculaires, Institut de Biologie Structurale et Microbiologie, CNRS, 31 chemin Joseph-Aiguier, 13402 Marseille cedex 20, France. duche@ibsm.cnrs-mrs.fr
D001426:Bacterial Proteins / Q000378:metabolism; D003087:Colicins / Q000737:chemistry* / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D019897:Periplasm / Q000378:metabolism; D018272:Porins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000502:physiology*; D017434:Protein Structure, Tertiary
D001426:Bacterial Proteins; D003087:Colicins; D018272:Porins; D021382:Protein Sorting Signals; C007104:colicin immunity proteins