title: Degradation of cinnamate via beta-oxidation to benzoate by a defined, syntrophic consortium of anaerobic bacteria.
abstract: A syntrophic consortium was enriched in a basal medium containing cinnamate as the carbon and energy source. It was found to consist of three morphologically distinct microbes, viz., a short, rod-shaped, non-motile bacterium with distinctly pointed ends, Papillibacter cinnamivorans; a rod-shaped, motile bacterium with rounded ends, Syntrophus sp.; and a methanoarchaeon, Methanobacterium sp. This methanogen was then replaced by a collection strain of Methanobacterium formicicum. A syntrophic interdependency of the three partners of the consortium was observed during growth on cinnamate. In the presence of bromoethanesulfonic acid (BESA), cinnamate was transformed to benzoate, whereas under methanogenic conditions without BESA, cinnamate was first transformed to benzoate via beta-oxidation and subsequently completely degraded into acetate, CH(4), and CO(2). Papillibacter cinnamivorans was responsible for benzoate production from cinnamate, whereas a syntrophic association between Syntrophus sp. and the methanogen degraded benzoate to acetate, CH(4), and CO(2). A new anaerobic degradation pathway of cinnamate into benzoate via beta-oxidation by a pure culture of P. cinnamivorans is proposed.
authors.affiliation: Laboratoire de Chimie et Environnement, Université de Provence, 3 Place Victor Hugo, 13331 Marseille cedex 03, France.
authors.forename: Sabria
authors.initials: S
authors.lastname: Defnoun
authors.forename: Maurice
authors.initials: M
authors.lastname: Ambrosio
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Garcia
authors.forename: Alfred
authors.initials: A
authors.lastname: Traoré
authors.forename: Marc
authors.initials: M
authors.lastname: Labat
mesh_terms: D001421:Bacteria, Anaerobic / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D001565:Benzoates / Q000032:analysis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002934:Cinnamates / Q000032:analysis / Q000378:metabolism*; D008958:Models, Molecular; D010084:Oxidation-Reduction
chemical_list: D001565:Benzoates; D002934:Cinnamates