Inhibition of cytokine production by the traditional oriental medicine, 'Gamcho-Sasim-Tang' in mitogen-stimulated peripheral blood mononuclear cells from Adamantiades-Behçet's patients.
Gamcho-Sasim-Tang (GS-Tang) is a traditional Chinese medication, which has been successfully used in Korea for the treatment of Adamantiades-Behçet's disease (ABD). We investigated the modulation effects of GS-Tang on cytokine production from phytohaemagglutinin-stimulated peripheral blood mononuclear cells of Behçet's patients. ABD is a systemic inflammatory disorder and might involve immune dysfunction. Cytokines involved in the regulation of inflammatory reactions and immune responses may play a role in the pathogenesis of ABD. GS-Tang (1 mg/ml) significantly inhibited the production of proinflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta), compared to absence of GS-Tang (by 42.0+/-6.6% inhibition for TNF-alpha and 95.9+/-5.7% for IL-1 beta, P<0.05). GS-Tang also inhibited the production of IFN-gamma, immunoregulatory T helper cell type 1 cytokine, by 80.2+/-5.3% (P=0.001). The inhibitory effects of GS-Tang on cytokine production showed dose-dependent manner. Our results suggest that GS-Tang might have anti-inflammatory and immuno-regulatory effects through the cytokine modulation.
Kim
Mi-Sun
MS
College of Pharmacy, Wonkwang University, and KI Co, Ltd, Iksan Jeonbuk 570-749, South Korea.
Chung
Hwan-Suck
HS
Lee
Jun-Gyoung
JG
Lim
Woon Ki
WK
Hwang
Chung-Yeon
CY
Lee
Eon-Jeong
EJ
Cho
Kwang-Ho
KH
Wi
Dac-Han
DH
Kim
Hyung-Min
HM
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000328:Adult; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D001528:Behcet Syndrome / Q000188:drug therapy*; D016207:Cytokines / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000187:drug effects; D007375:Interleukin-1 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D008517:Phytotherapy; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects
D000276:Adjuvants, Immunologic; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma