A luxCDABE-based bioluminescent bioreporter for the detection of phenol.
A bioluminescent reporter strain, Acinetobacter sp. DF4-8, was constructed for the detection of phenol by inserting a mopR-like promoter upstream of the Vibrio fischeri bioluminescent luxCDABE gene cassette in a modified mini-Tn5 construct. When introduced into the chromosome of Acinetobacter sp. DF4, the bioreporter produced a sensitive bioluminescent response to phenol at concentrations ranging from 2.5 to 100 ppm. This response was linear (R(2)=0.986) in the range from 20 to 90 ppm. A significant bioluminescent response was also recorded when strain DF4-8 was incubated with slurries from aged, phenol-contaminated soil.
Abd-El-Haleem
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The Center for Environmental Biotechnology, The University of Tennessee, Knoxville, TN 37996, USA.
Ripp
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Scott
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Sayler
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D000150:Acinetobacter / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D017930:Genes, Reporter / Q000235:genetics*; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D010088:Oxidoreductases; D019800:Phenol / Q000032:analysis*; D011401:Promoter Regions, Genetic; D012989:Soil Pollutants / Q000032:analysis; D013379:Substrate Specificity; D014733:Vibrio / Q000235:genetics*
D001426:Bacterial Proteins; D012989:Soil Pollutants; D019800:Phenol; D010088:Oxidoreductases; C055198:LuxC protein, Bacteria