An experimental approach for direct observation of the interaction of polyanions with sphingosine-containing giant vesicles.
A new approach for direct optical microscopy observation of polyanion interactions with bilayers of giant cationic liposomes (GUVs) was suggested. Polyanions as DNA, dextran sulfate (DS), heparin (H) and polyacrylic acids (PA) were locally delivered by a micropipette to a part of a giant unilamellar vesicle membrane. The phenomena were directly observed under optical microscope. GUVs, about 100 micro m in diameter, formed of phosphatidylcholines and up to 33 mol% of the natural bioactive cationic amphiphile sphingosine (Sph), were prepared by electroformation. The effects of water-soluble molecules with high negative linear charge density as dextran sulfate (DS), heparin (H) polyacrylic acids (PA) and adenosine-5'-triphosphoric acid (ATP) were compared with those of DNAs. The resulting membrane topology transformations were monitored in phase contrast, while the DNA distribution was followed in fluorescence. DNA-induced endocytosis-like membrane morphology transformation due to the DNA/lipid membrane local interactions was observed. The DS, H and PA induced membrane topology transformations similar to those of the DNAs, while ATP did not cause any detectable ones. The endocytosis mechanism involves the formation of ordered domains in the GUV membrane where some surface and charge asymmetries between the two membrane monolayers were created. The sizes of created polyanionic/cationic membrane domains depend on the form, length and elasticity of the adsorbed highly charged molecules. Endosome-including capacities of polyanionic molecules depend heavily on the high linear negative charge at a certain length. An original method for direct studying of the DNA/membrane interactions in autoadaptable giant liposome system imitating biological membrane interactions was forwarded. The model observations could also help for understanding events associated with cationic liposome/DNA complex formation in gene transfer processes.
Hristova
N I
NI
Institute of Biophysics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str, Bl.21, 1113, Sofia, Bulgaria.
Angelova
M I
MI
Tsoneva
I
I
D000180:Acrylic Resins / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D016264:Dextran Sulfate / Q000737:chemistry / Q000648:ultrastructure; D006493:Heparin / Q000737:chemistry / Q000648:ultrastructure; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D010713:Phosphatidylcholines / Q000737:chemistry; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry*; D013110:Sphingosine / Q000737:chemistry*
D000180:Acrylic Resins; D000255:Adenosine Triphosphate; D004247:DNA; D016264:Dextran Sulfate; D006493:Heparin; D008081:Liposomes; D010713:Phosphatidylcholines; D000071228:Polyelectrolytes; D011108:Polymers; D013110:Sphingosine; C006903:carbopol 940