title: Editing site recognition and nucleotide insertion are separable processes in Physarum mitochondria.
abstract: Insertional RNA editing in Physarum polycephalum is a complex process involving the specific addition of non-templated nucleotides to nascent mitochondrial transcripts. Since all four ribonucleotides are substrates for the editing activity(s), both the site of insertion and the identity of the nucleotide to be added at a particular position must be specified, but the signals for these events have yet to be elucidated. Here we report the occurrence of sporadic errors in RNAs synthesized in vitro. These mistakes, which include omission of encoded nucleotides as well as misinsertions, occur only on templates that support editing. The pattern of these misediting events indicates that editing site recognition and nucleotide addition are separable events, and that the recognition step involves features of the mitochondrial template that are required for editing. The larger deletions lack all templated nucleotides between editing sites, suggesting that the transcription/editing apparatus can "jump" from one insertion site to another, perhaps mediated by interactions with editing determinants, while smaller omissions most likely reflect misalignment of the transcript upon resumption of templated RNA synthesis.
authors.affiliation: Center for RNA Molecular Biology, Case Western Reserve University, 2109 Adelbert Road, School of Medicine, Cleveland, OH 44106, USA.
authors.forename: Elaine M
authors.initials: EM
authors.lastname: Byrne
authors.forename: Angela
authors.initials: A
authors.lastname: Stout
authors.forename: Jonatha M
authors.initials: JM
authors.lastname: Gott
mesh_terms: D000818:Animals; D020029:Base Pairing; D018076:DNA, Complementary / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D008928:Mitochondria / Q000378:metabolism; D016839:Physarum polycephalum / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D017393:RNA Editing; D016053:RNA, Protozoan / Q000378:metabolism*; D013698:Templates, Genetic
chemical_list: D018076:DNA, Complementary; D016054:DNA, Protozoan; D016053:RNA, Protozoan