title: Determination of capsaicinoids in topical cream by liquid-liquid extraction and liquid chromatography.
abstract: A reversed-phase liquid chromatography (LC) method has been developed, optimised and validated for the separation and quantitation of capsaicin (CP) and dihydrocapsaicin (DHCP) in a topical cream formulation. Sample preparation involves liquid-liquid extraction prior to LC analysis. The method uses a Hypersil C(18) BDS, 5 micrometer, 250x4.6 mm I.D. column maintained at 35 degrees C. The mobile phase comprises methanol, water, acetonitrile (ACN) and acetic acid (47:42:10:1, v/v/v/v) at a flow rate of 1.0 ml/min. Robustness was evaluated by performing a central composite face-centred design (CCF) experiment. The method shows good selectivity, linearity, sensitivity and repeatability. The conditions allow the separation and quantitation of CP and DHCP without interference from the other substances contained in the cream.
authors.affiliation: Laboratory for Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmacy, Katholieke Universiteit Leuven, E. Van Evenstraat 4, 3000, Leuven, Belgium.
authors.forename: Eliangiringa
authors.initials: E
authors.lastname: Kaale
authors.forename: Ann
authors.initials: A
authors.lastname: Van Schepdael
authors.forename: Eugène
authors.initials: E
authors.lastname: Roets
authors.forename: Jos
authors.initials: J
authors.lastname: Hoogmartens
mesh_terms: D000287:Administration, Topical; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D002853:Chromatography, Liquid / Q000379:methods; D009824:Ointments
chemical_list: D009824:Ointments; D002211:Capsaicin; C012906:dihydrocapsaicin