Attenuation of fungal infection in thermoregulating Locusta migratoria is accompanied by changes in hemolymph proteins.
Hemolymph proteins in the locust, Locusta migratoria migratorioides infected with the fungus Metarhizium anisopliae var acridum were analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Under conditions that allowed locusts to thermoregulate, 2 proteins, ITB1 (ca. 18kDa) and ITB2 (ca. 13kDa) were induced 48h post inoculation. In contrast, under non-thermoregulating conditions, only 1 band, INTB1 (ca. 18kDa) was induced with similar molecular mass to ITB1. ITB1 and ITB2 were N-terminally sequenced but showed little homology to known proteins. The induction of hemolymphal proteins in infected, thermoregulating locusts and implication in insect immune defence are discussed.
Ouedraogo
Robert M
RM
Département de Phytologie, Université Laval, Sainte-Foy, G1K 7P4, Québec, Qc, Canada.
Kamp
Andrena
A
Goettel
Mark S
MS
Brodeur
Jacques
J
Bidochka
Michael J
MJ
D000818:Animals; D001833:Body Temperature Regulation; D006110:Grasshoppers / Q000382:microbiology* / Q000502:physiology*; D006458:Hemolymph / Q000378:metabolism*; D009181:Mycoses / Q000503:physiopathology*; D011506:Proteins / Q000378:metabolism
D011506:Proteins