title: [Preimplantation genetic diagnosis: update of the Parisian group].
abstract: To report the birth of the first fourteen infants conceived after preimplantation genetic diagnosis (PGD) in our unit. Fifty-nine couples were enrolled between January 2000 and July 2001. They had a total of 71 oocyte pick-up cycles. The collected oocytes were inseminated by intracytoplasmic sperm injection. The resulting embryos were biopsied on the third day of development and the genetic analysis was performed on the same day. Most of the embryo transfers were carried out on the fourth day. The 71 oocyte pick-up cycles yielded 872 oocytes of which 731 were suitable for intracytoplasmic sperm injection. Among the 505 embryos obtained, 421 embryos were biopsied and genetic diagnosis was performed for 312 (74%) of these 127 embryos were transferred during the course of 58 transfer procedures. There were 18 biochemical and 12 ongoing (7 singles, 4 twins and 1 triple) pregnancies. Sixteen infants have been born and 2 are expected. PGD has gained a place among the choices offered to couples at risk of transmission of a serious and incurable genetic disease.
authors.affiliation: Service d'Histologie-Embryologie-Cytogénétique à orientation Biologique et Génétique de la Reproduction, Hôpital Antoine Béclère (AP-HP), 157, rue de la Porte de Trivaux-92141 Clamart.
authors.forename: René
authors.initials: R
authors.lastname: Frydman
authors.forename: Gérard
authors.initials: G
authors.lastname: Tachdjian
authors.forename: Nelly
authors.initials: N
authors.lastname: Achour-Frydman
authors.forename: Pierre
authors.initials: P
authors.lastname: Ray
authors.forename: Serge
authors.initials: S
authors.lastname: Romana
authors.forename: Samir
authors.initials: S
authors.lastname: Hamamah
authors.forename: Sabine
authors.initials: S
authors.lastname: Marcadet-Fredet
authors.forename: Violaine
authors.initials: V
authors.lastname: Kerbrat
authors.forename: Renato
authors.initials: R
authors.lastname: Fanchin
authors.forename: Jacques
authors.initials: J
authors.lastname: Kadoch
authors.forename: Tania
authors.initials: T
authors.lastname: Attie
authors.forename: M
authors.initials: M
authors.lastname: Lelorc'h
authors.forename: Michel
authors.initials: M
authors.lastname: Vekemans
authors.forename: Arnold
authors.initials: A
authors.lastname: Munnich
mesh_terms: D000328:Adult; D002873:Chromosome Fragility; D020732:Cytogenetic Analysis; D005260:Female; D006801:Humans; D008297:Male; D009865:Oocytes; D011247:Pregnancy; D011256:Pregnancy Outcome; D019836:Preimplantation Diagnosis; D012307:Risk Factors; D020554:Sperm Injections, Intracytoplasmic