[Relative quantitative analysis of corneal immunogenicity].
OBJECTIVE
To quantitate the relative immunogenicity of three major corneal cell layers (epithelium, stroma, and endothelium) among the whole corneal immunogenicity respectively.


METHODS
Cellular immunity: Three porcine major corneal cell layers were heterotopically allografted to subcutaneous layer of 40 BALB-c mice respectively. Twelve days later, the peripheral white blood cells of recipients were double directly stained with anti-mouse IgG-fluorescein conjugate mAb and analyzed by flow cytometry. Humoral immunity: The suspension of porcine cornea or corneal epithelium was used as antigen to produce anti-porcine immune sera in C57BL-6 mice. With the anti-porcine immune sera, enzyme linked immunosorbent assay was performed.


RESULTS
On cellular immunity detection, the immunogenicities of intact endothelium, epithelium and stroma, equal in thickness, were 70.75%, 27.63%, and 1.62% respectively. On humoral immunity detection, the immunogenicities were 62.11%, 31.77% and 6.12% in the three respective layers of equal thickness.


CONCLUSIONS
These findings indicate that the immunogenicity of corneal stroma is the lowest among the three corneal cell layers of equal thickness. The preliminary results provide the experimental basis for clinical application of xenogenic graft of corneal stroma.
Wang
Zhichong
Z
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China. zcwang59@hotmail.com
Ge
Jian
J
Xu
Jintang
J
Chen
Jiaqi
J
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D003315:Cornea / Q000166:cytology / Q000276:immunology*; D016039:Corneal Transplantation / Q000276:immunology* / Q000379:methods; D004727:Endothelium / Q000166:cytology / Q000276:immunology / Q000637:transplantation; D004847:Epithelial Cells / Q000276:immunology / Q000637:transplantation; D005434:Flow Cytometry; D007111:Immunity, Cellular / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D017154:Stromal Cells / Q000276:immunology / Q000637:transplantation; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology; D014183:Transplantation, Heterologous