Effects of cyclosporin A on the levels of intercellular adhesion molecule-1 expressed by oral fibroblasts.
OBJECTIVE
To investigate the effects of cyclosporin A (CSA) on the level of intercellular adhesion molecule-1 (ICAM-1) expressed by oral fibroblasts (FB).


METHODS
The fibroblasts were obtained from normal buccal mucosa (NM-FB) and cultured in vitro. Then the levels of ICAM-1 expressed by fibroblasts incubated with or without CSA in the presence of 10% fetal calf serum for 48 hours at 37 degrees C in 5% CO(2) and air were monitored by using cell-based ELISA for ICAM-1.


RESULTS
We demonstrated that NM-FB had ICAM-1 expression (A = 0.324 +/- 0.030), and after 48 hours incubated with CSA the ICAM-1 levels expressed by oral buccal mucosa fibroblasts were dose-dependently decreased.


CONCLUSIONS
CSA reduces the levels of ICAM-1 expressed by oral mucosal FB, and may be useful in the treatment of some oral mucosal disorders.
Feng
Yunzhi
Y
Department of Prosthodonties, Peking University School of Stomatology, Beijing 100081, China.
Ling
Tianyou
T
Wu
Hanjiang
H
Li
Yunliang
Y
Liu
Zhiwen
Z
D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D009061:Mouth Mucosa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D007166:Immunosuppressive Agents; D018799:Intercellular Adhesion Molecule-1; D016572:Cyclosporine