Immunolocalization of PTCH protein in odontogenic cysts and tumors.
The human patched gene (PTCH) functions in both embryologic development and tumor suppression. PTCH mutations have been found in odontogenic keratocysts. However, the expression and localization of the protein product of the gene have not been determined in odontogenic tumors and cysts. We investigated 68 odontogenic lesions by immunohistochemistry, and compared their PTCH expression with that in basal cell carcinomas. All odontogenic lesions, including two keratocysts with truncating mutations, were positive for PTCH. Different types of lesions had different amounts of staining. Lack of staining was noted in the majority of basal cell carcinomas. Taken together, these data suggest that odontogenic keratocysts arise with heterozygous mutations of the PTCH gene.
Barreto
D C
DC
Departmento de Clínica, Patologia e Cirurgia, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte-MG, Brazil.
Bale
A E
AE
De Marco
L
L
Gomez
R S
RS
D000595:Amino Acid Sequence; D002280:Basal Cell Carcinoma / Q000737:chemistry / Q000235:genetics; D016147:Genes, Tumor Suppressor; D006579:Heterozygote; D006801:Humans; D007124:Immunoenzyme Techniques; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics; D009807:Odontogenic Cysts / Q000737:chemistry / Q000235:genetics*; D009808:Odontogenic Tumors / Q000737:chemistry / Q000235:genetics*; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; D011956:Receptors, Cell Surface
D008565:Membrane Proteins; D009363:Neoplasm Proteins; C000606236:PTCH1 protein, human; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; D011956:Receptors, Cell Surface