title: Two enzyme-linked immunosorbent assay (ELISA) systems for N1,N8-diacetylspermidine and N1,N12-diacetylspermine using monoclonal antibodies.
abstract: We obtained monoclonal antibodies against N(1),N(12)-diacetylspermine (DiAcSpm) and N(1),N(8)-diacetylspermidine (DiAcSpd), and developed two systems of competitive ELISA that utilize the antibodies and a common enzyme-labeled antigen to measure these di-acetylpolyamines. Cross-reactions with N(1)-acetylspermidine in the assay of DiAcSpm and with N8-acetylspermidine in the assay of DiAcSpd were as low as 0.26 and 0.6%, respectively, and were judged to be insignificant in clinical use for measuring urinary diacetylpolyamines. These assays were used to assess diurnal variations in diacetylpolyamine excretion in urine to show that the excretion of diacetylpolyamines after normalization for the concentration of creatinine is stable over a day with only minimal diurnal variation.
authors.affiliation: Immunology Laboratory, Diagnostics Department, Yamasa Corporation, Araoi-cho, Choshi, Chiba 288-0056. hamaoki@yamasa.com
authors.forename: Masaru
authors.initials: M
authors.lastname: Hamaoki
authors.forename: Kyoko
authors.initials: K
authors.lastname: Hiramatsu
authors.forename: Setsuo
authors.initials: S
authors.lastname: Suzuki
authors.forename: Atsuo
authors.initials: A
authors.lastname: Nagata
authors.forename: Masao
authors.initials: M
authors.lastname: Kawakita
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D013095:Spermidine / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology*; D013096:Spermine / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; C093909:N(1),N(8)-diacetylspermidine; D013096:Spermine; C029453:N',N''-diacetylspermine; D013095:Spermidine