title: Mulberry anthracnose antagonists (iturins) produced by Bacillus amyloliquefaciens RC-2.
abstract: Bacillus amyloliquefaciens strain RC-2 produced seven antifungal compounds (1-7) secreted into the culture filtrate. These compounds inhibited the development of mulberry anthracnose caused by the fungus, Colletotrichum dematium. Chemical structural analyses by NMR and FAB-MS revealed that all these compounds were iturins (cyclic peptides with the following sequence: L-Asn --> D-Tyr --> D-Asn --> L-Gln --> L-Pro --> D-Asn --> L-Ser --> D-beta-amino acid -->) and compounds 1-6 are identical to iturins A-2-A-7, respectively. Compound 7 (iturin A-8) is a new iturin, which has a -(CH(2))(10)CH(CH(3))CH(2)CH(3) group as a side chain in the beta-amino acid in the molecule.
authors.affiliation: Department of Biological Safety Science, National Institute for Agro-Environmental Sciences, 3-1-3 Kan-nondai, Tsukuba, Ibaraki 305-8604, Japan. hiradate@affrc.go.jp
authors.forename: Syuntaro
authors.initials: S
authors.lastname: Hiradate
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Yoshida
authors.forename: Hajime
authors.initials: H
authors.lastname: Sugie
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yada
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Fujii
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001407:Bacillus / Q000254:growth & development / Q000378:metabolism*; D005658:Fungi / Q000187:drug effects*; D031625:Morus / Q000382:microbiology*; D010456:Peptides, Cyclic / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D013057:Spectrum Analysis
chemical_list: D000935:Antifungal Agents; D010456:Peptides, Cyclic