title: The use of single-donor fibrin glue prepared by recycled cryoprecipitation in experimental liver surgery.
abstract: The purpose of the study was to evaluate the hemostatic effectiveness of fibrin glue (FG) prepared by a modification of cryoprecipitation technique in experimental rat liver surgery. FG component 1 was prepared by triple or 'recycled' cryoprecipitation method from single-donor plasma. Rats subjected to liver incision, partial and total lobectomy were treated with FG on the surgical cut surface or underwent standard surgical technique. The efficacy of FG-treatment was evaluated on the basis of the 24-hour survival ratio and peripheral blood hematological parameters. The mean values of fibrinogen, FXIII, fibronectin and horizontal tensile strength of FG were 54.2 +/- 19.9 g/l, 13.5 +/- 3.6 IU/ml, 3103.1 +/- 148.9 mg/l, and 1.076 +/- 0.18 N/cm2, respectively. The survival of FG-treated rats subjected to partial and total lobectomy was significantly higher in comparison to the FG-nontreated animals, accompanied with higher values of red blood cell counts, hemoglobin concentration and hematocrit. When liver incision was performed, although there were no differences in survival rate, FG-treated animals had significantly higher values of the tested hematological parameters. The presented results demonstrated that by using 'recycled' cryoprecipitation it is possible to obtain high quality single-donor FG with successful hemostatic therapeutical effects, as confirmed in the experimental rat model of liver surgery.
authors.affiliation: Institute of Transfusiology of MMA, Belgrade, Yugoslavia. belab@imi.bg.ac.yu
authors.forename: Bela
authors.initials: B
authors.lastname: Balint
authors.forename: Ibolja
authors.initials: I
authors.lastname: Cernak
authors.forename: Marijana
authors.initials: M
authors.lastname: Petakov
authors.forename: Diana
authors.initials: D
authors.lastname: Bugarski
authors.forename: Zivorad
authors.initials: Z
authors.lastname: Malićević
authors.forename: Slavka
authors.initials: S
authors.lastname: Mandic-Radić
authors.forename: Jovan
authors.initials: J
authors.lastname: Taseski
mesh_terms: D000818:Animals; D011232:Chemical Precipitation; D003080:Cold Temperature; D013505:Digestive System Surgical Procedures / Q000379:methods*; D004353:Drug Evaluation, Preclinical; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology* / Q000627:therapeutic use; D006403:Hematologic Tests; D006488:Hemostasis, Surgical / Q000379:methods; D008099:Liver / Q000601:surgery*; D051381:Rats; D015996:Survival Rate
chemical_list: D015718:Fibrin Tissue Adhesive