title: Structure of the O-specific polysaccharide of Providencia alcalifaciens O16 containing N-acetylmuramic acid.
abstract: The O-specific polysaccharide of Providencia alcalifaciens O16 was obtained by mild-acid degradation of the lipopolysaccharide and studied by chemical methods and NMR spectroscopy, including 2D 1H,(1)H COSY, TOCSY, NOESY, and 1H,(13)C HSQC experiments. It was found that the polysaccharide contains N-acetylmuramic acid, which was isolated by solvolysis with trifluoromethanesulfonic acid and identified by the specific optical rotation and NMR spectroscopy. The following structure of the trisaccharide repeating-unit of the polysaccharide was established:
authors.affiliation: ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991, GSP-1, Moscow, Russia.
authors.forename: Nina A
authors.initials: NA
authors.lastname: Kocharova
authors.forename: George V
authors.initials: GV
authors.lastname: Zatonsky
authors.forename: Olga V
authors.initials: OV
authors.lastname: Bystrova
authors.forename: Andrzej
authors.initials: A
authors.lastname: Ziolkowski
authors.forename: Marianna
authors.initials: M
authors.lastname: Wykrota
authors.forename: Aleksander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
authors.forename: Antoni
authors.initials: A
authors.lastname: Rozalski
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D009112:Muramic Acids / Q000737:chemistry* / Q000302:isolation & purification*; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry*; D009904:Optical Rotation; D011532:Providencia / Q000737:chemistry* / Q000145:classification
chemical_list: D009112:Muramic Acids; D019081:O Antigens; C031651:N-acetylmuramic acid