Enzymatic hydrolysis of molasses.
Kinetic studies of the enzymatic hydrolysis of molasses were conducted using glucoamylase. Central Sugar Refinery SDN BHD contains 13-20% glucose. The molasses was diluted and the kinetic experiments were conducted at 67 degrees C with 100-1000 mg/l of glucoamylase. The glucose contents of the molasses were enhanced after hydrolysis of molasses solution with 1000 mg/l glucoamylase. A Lineweaver-Burk plot was obtained based on enzyme kinetic data. The rate constant, Km and maximum reaction rate, Vmax for 500 mg/l of glucoamylase were 100 mmol/l (18 g/l) and 5 mmol/l min (0.9 g/l min), respectively. The maximum reaction rate, Vmax for 1000 mg/l of glucoamylase was doubled, to 100 mmol/l (18 g/l) and the rate constant, Km was the same for 500 mg/l of glucoamylase. The substrate inhibition model was noncompetitive based on the resulting Lineweaver-Burk plot for enzyme concentration of 500 and 1000 mg/l.
Najafpour
Ghasem D
GD
School of Chemical Engineering, Universiti Sains Malaysia, Pulau Pinang. najafpour@hotmail.com
Shan
Cheong Poi
CP
D003116:Color; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008965:Molasses / Q000032:analysis; D014867:Water / Q000032:analysis
D014867:Water; D005087:Glucan 1,4-alpha-Glucosidase