Nitric oxide controls the light adaptive chromatic difference in receptive field size of H1 horizontal cell network in carp retina.
In carp retina, the receptive field size of the H1-type horizontal cell (HC) network is known to be chromatically selective, as electrophysiological signals are generated by short-wavelength (SW) light stimuli, which spread much less than those for long-wavelength (LW). We have shown previously that the signalling mechanism underlying this chromatic difference operates only in the light-adapted retina and that it involves cGMP as an intermediary messenger. In the present study, the possible role of nitric oxide (NO) as such a control mechanism was investigated. Application of a NO donor (SNP or SNOG) to dark-adapted retinae produced a chromatic difference in the receptive field size, such as in the light-adapted state. This effect was due mainly to a reduction in the spread of signals generated by SW stimuli; LW signalling spread was not altered. No such effect was observed in light-adapted retinae where a chromatic difference in receptive field size was already present. On the other hand, application of the NO 'scavenger' haemoglobin to light-adapted retinae suppressed the chromatic difference. These results are consistent with NO being a light-adaptive retinal neuromodulator involved in the generation of the chromatic difference in H1 cell receptive field size. These results are discussed in the context of two different hypotheses.
Furukawa
Tetsuo
T
Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan.
Petruv
Renata
R
Yasui
Syozo
S
Yamada
Masahiro
M
Djamgoz
Mustafa B A
MB
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000221:Adaptation, Ocular / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D002347:Carps; D046508:Culture Techniques; D003623:Dark Adaptation / Q000187:drug effects / Q000502:physiology; D004594:Electrophysiology / Q000379:methods; D009415:Nerve Net / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D010775:Photic Stimulation; D011930:Reaction Time; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D026422:S-Nitrosoglutathione / Q000494:pharmacology
D020030:Nitric Oxide Donors; D009599:Nitroprusside; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione