Synthesis of long Poly(dA).Poly(dT) DNA without structural defects using enzymatic reaction; tailored ligated Poly(dA).Poly(dT).
Poly(dA).Poly(dT) molecules up to 1000 base pairs (bp) have been synthesized using enzymatic reaction, and characterization by STM observation shows that the DNA has no defects in the duplex structure.
Tanaka
Shin-ichi
S
Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
Fujiwara
Shinsuke
S
Tanaka
Hiroyuki
H
Taniguchi
Masateru
M
Tabata
Hitoshi
H
Fukui
Kiichi
K
Kawai
Tomoji
T
D004259:DNA-Directed DNA Polymerase / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D017348:Microscopy, Electron, Scanning Transmission; D009690:Nucleic Acid Conformation; D011067:Poly dA-dT / Q000138:chemical synthesis* / Q000737:chemistry; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000737:chemistry; D011130:RNA Ligase (ATP) / Q000737:chemistry; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000737:chemistry
D011067:Poly dA-dT; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D004259:DNA-Directed DNA Polymerase; D015719:Single-Strand Specific DNA and RNA Endonucleases; D011130:RNA Ligase (ATP)