title: Successful single donor islet allotransplantation in the streptozotocin diabetes rat model.
abstract: The objective of this study was to define pretransplant islet culture conditions for optimum tissue engraftment in the rat islet allotransplantation model. Lewis rat islets were cultured in TCM 199/5% fetal calf serum for I day at 37 degrees C, followed by 1 day of culture at 22 degrees C. When islets from single donors were allotransplanted intraportally into single streptozotocin-diabetic Wistar-Furth rats, complete normoglycemia was restored within 1 day after transplantation in seven out of seven rats, and persisted up to immunological rejection about 1 week later. In five out of six rats receiving a posttransplant injection of antilymphocyte serum, plasma glucose was normalized for >100 days. These data demonstrate, for the first time, successful single-donor-to-single-recipient transplantation of allogeneic rat pancreatic islets. Because islet engraftment may still be regarded as a main problem for clinical islet transplantation, the pretransplant islet culture regimen outlined in this article may lead to a more efficient use of donor pancreatic islet tissue in the clinical setting, as well.
authors.affiliation: Third Medical Department, University of Giessen, Germany. Henning.Jahr@innere.med.uni-giessen.de
authors.forename: Henning
authors.initials: H
authors.lastname: Jahr
authors.forename: Birte
authors.initials: B
authors.lastname: Hussmann
authors.forename: Torsten
authors.initials: T
authors.lastname: Eckhardt
authors.forename: Reinhard G
authors.initials: RG
authors.lastname: Bretzel
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D006084:Graft Rejection; D007515:Islets of Langerhans / Q000166:cytology; D016381:Islets of Langerhans Transplantation / Q000276:immunology / Q000379:methods* / Q000502:physiology; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D011920:Rats, Inbred WF; D013154:Spleen / Q000166:cytology; D013997:Time Factors; D014184:Transplantation, Homologous
chemical_list: D001786:Blood Glucose