[Distribution of hemorrhagic fever with renal syndrome virus in gamasid mites and chigger mites].
OBJECTIVE
To study the distribution of hemorrhagic fever with renal syndrome virus (HFRSV) in mites.


METHODS
In situ reverse transcription-polymerase chain reaction (IS RT-PCR) was used for detecting the distribution of HFRSV in mites.


RESULTS
HFRSV RNA was mainly located in ovary and mid-gut tissues of gamasid mites and chigger mites. The positive signal intensity in the third and fourth generations of gamasid mite was stronger than that in the first and second generations, and that in nymph of chigger mite more than larva.


CONCLUSIONS
Both chigger mite and gamasid mite could play an important role in the transmission of HFRSV.
Zhang
Yun
Y
Institute of Military Medicine, Nanjing Command of PLA, Nanjing 210002, China.
Zhu
Jin
J
Deng
Xiaozhao
X
Wu
Guanghua
G
Zhang
Jiaju
J
Zhou
Yanping
Y
D000818:Animals; D004064:Digestive System / Q000821:virology; D005260:Female; D018063:Hantaan virus / Q000235:genetics* / Q000254:growth & development; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D008925:Mites / Q000821:virology*; D009758:Nymph / Q000821:virology; D010053:Ovary / Q000821:virology; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D012367:RNA, Viral