title: Synthesis of long Poly(dA).Poly(dT) DNA without structural defects using enzymatic reaction; tailored ligated Poly(dA).Poly(dT).
abstract: Poly(dA).Poly(dT) molecules up to 1000 base pairs (bp) have been synthesized using enzymatic reaction, and characterization by STM observation shows that the DNA has no defects in the duplex structure.
authors.affiliation: Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Tanaka
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Fujiwara
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Masateru
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Tabata
authors.forename: Kiichi
authors.initials: K
authors.lastname: Fukui
authors.forename: Tomoji
authors.initials: T
authors.lastname: Kawai
mesh_terms: D004259:DNA-Directed DNA Polymerase / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D017348:Microscopy, Electron, Scanning Transmission; D009690:Nucleic Acid Conformation; D011067:Poly dA-dT / Q000138:chemical synthesis* / Q000737:chemistry; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000737:chemistry; D011130:RNA Ligase (ATP) / Q000737:chemistry; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000737:chemistry
chemical_list: D011067:Poly dA-dT; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D004259:DNA-Directed DNA Polymerase; D015719:Single-Strand Specific DNA and RNA Endonucleases; D011130:RNA Ligase (ATP)