Extract and identify ingredient from Ligustrum Lucidum Ait and study its effect to periodontal pathogen.
OBJECTIVE
To extract the effective ingredient (crystal I) from effective section (saponin) of Ligustrum Lucidum Ait, identify the chemical structure of crystal I, study the effect of crystal I on P. gingivalis, B. forsythus and P. intermedia.


METHODS
Isolated crystal I from saponin using the silica gel column chromatograph. Identified crystal I with IR spectra, (1)H-NMR and (13)C-NMR. Measured the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) through micro-liquid dilution. Studied the killing curve of ursolic acid on B. forsythus and P. intermedia.


RESULTS
The crystal I was identified as ursolic acid; its MIC and MBC to P. gingivalis, B. forsythus and P. intermedia were 0.740 and 0.295 microg/L respectively. The killing curve indicated that 0.800 microg/L ursolic acid could kill P. intermedia and B. forsythus in 3 and 6 hours respectively.


CONCLUSIONS
Ursolic acid has obvious effect to inhibit periodontal pathogen.
Wang
Qian
Q
Department of Operative Dentistry and Endodontics, School of Stomatology, Wuhan University, Wuhan 430079, China.
Fan
Mingwen
M
Bian
Zhuan
Z
Nie
Min
M
Chen
Zhi
Z
D001439:Bacteroides / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D031659:Ligustrum; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008826:Microbial Sensitivity Tests; D010519:Periodontium / Q000382:microbiology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D016966:Porphyromonas gingivalis / Q000187:drug effects; D018713:Prevotella intermedia / Q000187:drug effects; D014315:Triterpenes / Q000494:pharmacology; D000097245:Ursolic Acid
D010936:Plant Extracts; D014315:Triterpenes; D000097245:Ursolic Acid