Resveratrol inhibits proliferation and induces apoptosis in human pancreatic cancer cells.
BACKGROUND
Because of lack of early diagnosis and poor therapeutic responsiveness, median survival in patients with pancreatic cancer is <6 months, and survival beyond 5 years is rare. Thus, another dimension in chemotherapeutic agents for pancreatic cancer would be beneficial to control metastatic and unresectable disease. Resveratrol, a natural product from grapes, has been shown to be chemopreventive for carcinogen-induced skin cancer and also to inhibit proliferation of oral squamous, breast, colonic, and prostate cancer cells.


OBJECTIVE
To investigate the effect of resveratrol in pancreatic cancer.


METHODS
To evaluate the potential role of resveratrol on pancreatic cancer cell proliferation, two human pancreatic cancer cell lines, PANC-1 and AsPC-1, were used.


RESULTS
Resveratrol inhibited proliferation of both PANC-1 and AsPC-1 in a concentration- and time-dependent manner as measured by [ H]thymidine incorporation. Cell number of both PANC-1 and AsPC-1 was also significantly decreased following 48 and 72 hours of treatment with 100 micromol/L resveratrol. The growth inhibition induced by resveratrol was accompanied by apoptotic morphologic changes, characterized by cell rounding and cell membrane blebbing suggesting apoptosis. Propidium iodide staining of DNA, measured by flow cytometry, showed a dramatic increase in the fraction of sub-G0/G1 cells following resveratrol treatment in both PANC-1 and AsPC-1. The substantial apoptosis inducted by resveratrol on these two cell lines was confirmed by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling assay.


CONCLUSIONS
These findings suggest that the natural product resveratrol may have a potent antiproliferative effect on human pancreatic cancer with induction of apoptosis. Resveratrol is likely to be valuable for the management and prevention of human pancreatic cancer.
Ding
Xian-Zhong
XZ
Department of Surgery, Northwestern University Medical School, Chicago, Illinois, USA
Adrian
Thomas E
TE
D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D017209:Apoptosis; D002455:Cell Division / Q000187:drug effects; D004396:Coloring Agents / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020287:In Situ Nick-End Labeling; D007700:Kinetics; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D011419:Propidium / Q000737:chemistry; D000077185:Resveratrol; D013267:Stilbenes / Q000627:therapeutic use*; D013936:Thymidine / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D016588:Anticarcinogenic Agents; D004396:Coloring Agents; D013267:Stilbenes; D011419:Propidium; D004247:DNA; D000077185:Resveratrol; D013936:Thymidine