title: Degradation of environmental pollutants by Trametes trogii.
abstract: The ability of the ligninolytic fungus Trametes trogii to degrade in vitro different xenobiotics (PCBs, PAHs and dyes) was evaluated. Either 200 ppm of a PCB mixture (Aroclor 1150) or 160 ppm of an industrial PAH mixture (10% V/V of PAHs, principal components hexaethylbenzene, naphthalene, 1-methyl naphthalene, acenaphthylene, anthracene, fluorene and phenanthrene), were added to trophophasic and idiophasic cultures growing in a nitrogen limited mineral medium (glucose/asparagine) and in a complex medium (malt extract/glucose). Gas-liquid chromatography proved that within 7 to 12 d more than 90% of the organopollutants added were removed. The decrease in absorbance at 620 nm demonstrated that cultures of this fungus were able to transform 80% of the dye Anthraquinone-blue (added at a concentration of 50 ppm) in 1.5 h. Enzyme estimations indicated high activity of laccase (up to 0.55 U/mL), as well as lower production of manganese-peroxidase. Laccase activity, detected in all the conditions assayed, could be implicated in the degradation of these organopollutants. Considering the results obtained, T. trogii seems promising for detoxification.
authors.affiliation: Laboratorio de Microbiología, Departamento de Química Biológica, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina.
authors.forename: C
authors.initials: C
authors.lastname: Haglund
authors.forename: L
authors.initials: L
authors.lastname: Levín
authors.forename: F
authors.initials: F
authors.lastname: Forchiassin
authors.forename: M
authors.initials: M
authors.lastname: López
authors.forename: A
authors.initials: A
authors.lastname: Viale
mesh_terms: D001140:Aroclors / Q000378:metabolism; D001673:Biodegradation, Environmental; D002617:Chemical Industry; D004396:Coloring Agents / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006841:Hydrocarbons, Aromatic / Q000378:metabolism; D007220:Industrial Waste; D042845:Laccase; D010088:Oxidoreductases / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000378:metabolism; D020072:Polyporales / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism*; D015262:Xenobiotics / Q000378:metabolism
chemical_list: D001140:Aroclors; D004396:Coloring Agents; D005656:Fungal Proteins; D006841:Hydrocarbons, Aromatic; D007220:Industrial Waste; D012989:Soil Pollutants; D015262:Xenobiotics; D011078:Polychlorinated Biphenyls; D010088:Oxidoreductases; D042845:Laccase