[Relationship between human leukocyte antigen-DRB1 allele gene polymorphism and intrahepatic cholestasis of pregnancy].
OBJECTIVE
To study the relationship between human leukocyte antigen-DRB1 (HLA-DRB1) allele genes polymorphism and intrahepatic cholestasis of pregnancy (ICP).


METHODS
Forty-two patients with ICP were tested for HLA-DRB1 allele genes polymorphism with the polymerase chain reaction technique and sequence specific oligonucleotide (PCR-SSO) probes hybridization, 56 normal pregnant women as control group were also tested. In addition, the phenotype frequencies of HLA-DRB1 alleles were compared with it's clinical character in patients with ICP.


RESULTS
The higher frequencies were observed for alleles DR9, DR12 and DR4 in both groups. DR6 alleles were detected in 14 cases out of 42 patients. Patients with ICP had a significantly higher frequency of the allele DR6 when compared to control group (16.7% vs 3.6%), with a relative risk (RR) as 6.5 (P < 0.01). No significant differences were observed between the frequencies of other detected HLA-DRB1 alleles in both groups. There was no association between HLA-DR6 allele and the level of liver function and cholylglycine in ICP.


CONCLUSIONS
The study showed that HLA-DR6 gene might be one of the susceptibility genes to ICP.
Chen
Fuqiang
F
Department of Obstetrics, Shanghai Pudong New Area Health Hospital For Women and Children, Shanghai 201200, China.
Fan
Lian
L
Xu
Lingdi
L
Yao
Fangjuan
F
D000328:Adult; D000483:Alleles; D002780:Cholestasis, Intrahepatic / Q000150:complications / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006684:HLA-DR Antigens / Q000235:genetics*; D059810:HLA-DR Serological Subtypes; D015804:HLA-DR4 Antigen / Q000235:genetics; D015808:HLA-DR6 Antigen / Q000235:genetics; D059811:HLA-DRB1 Chains; D006801:Humans; D011110:Polymorphism, Genetic; D011247:Pregnancy; D011248:Pregnancy Complications
D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C082411:HLA-DR12 antigen; D015804:HLA-DR4 Antigen; D015808:HLA-DR6 Antigen; D059811:HLA-DRB1 Chains