NPY modulates epinephrine-induced leukocytosis via Y-1 and Y-5 receptor activation in vivo: sympathetic co-transmission during leukocyte mobilization.
Sympathetic nervous system (SNS) activation mobilizes blood leukocytes. Under these circumstances, both epinephrine (EPI) and neuropeptide Y (NPY) are released. Therefore, we investigated a possible interaction between these transmitters during leukocyte mobilization, using intravenous catheterization of male adult Lewis rats. Intravenous application of NPY followed by EPI, dose-dependently facilitated, intensified and inhibited EPI-induced leukocytosis with subset-specificity for NK-cells, monocytes, and B-lymphocytes. Pharmacological assessment of NPY receptors involved revealed a Y-1R-mediated inhibition and a Y-5R-mediated facilitation. RT-PCR on peripheral blood mononuclear cells (PBMC) detected Y-1R mRNA only, suggesting direct Y-1R-mediated effects on leukocytes and indirect effects via the Y-5R. Thus, via a specific Y-1R/Y-5R interplay, NPY acts as a neuroimmune co-transmitter in vivo.
Bedoui
Sammy
S
Department of Functional and Applied Anatomy, Hannover Medical School, 30625 Hanover, Germany.
Lechner
Sandra
S
Gebhardt
Thomas
T
Nave
Heike
H
Beck-Sickinger
Annette G
AG
Straub
Rainer H
RH
Pabst
Reinhard
R
von Hörsten
Stephan
S
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects*; D007964:Leukocytosis / Q000139:chemically induced*; D008297:Male; D009478:Neuropeptide Y / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011917:Rats, Inbred Lew; D017476:Receptors, Neuropeptide Y / Q000235:genetics / Q000502:physiology*; D009435:Synaptic Transmission
D009478:Neuropeptide Y; D012333:RNA, Messenger; D017476:Receptors, Neuropeptide Y; C097212:neuropeptide Y-Y1 receptor; C100568:neuropeptide Y5 receptor; D004837:Epinephrine