title: Transcript analysis of genes encoding a family 61 endoglucanase and a putative membrane-anchored family 9 glycosyl hydrolase from Phanerochaete chrysosporium.
abstract: Phanerochaete chrysosporium cellulase genes were cloned and characterized. The cel61A product was structurally similar to fungal endoglucanases of glycoside hydrolase family 61, whereas the cel9A product revealed similarities to Thermobifida fusca Cel9A (E4), an enzyme with both endo- and exocellulase characteristics. The fungal Cel9A is apparently a membrane-bound protein, which is very unusual for microbial cellulases. Transcript levels of both genes were substantially higher in cellulose-grown cultures than in glucose-grown cultures. These results show that P. chrysosporium possesses a wide array of conventional and unconventional cellulase genes.
authors.affiliation: Department of Bacteriology. Department of Chemical Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
authors.forename: Amber Vanden
authors.initials: AV
authors.lastname: Wymelenberg
authors.forename: Stuart
authors.initials: S
authors.lastname: Denman
authors.forename: Diane
authors.initials: D
authors.lastname: Dietrich
authors.forename: Jennifer
authors.initials: J
authors.lastname: Bassett
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Yu
authors.forename: Rajai
authors.initials: R
authors.lastname: Atalla
authors.forename: Paul
authors.initials: P
authors.lastname: Predki
authors.forename: Ulla
authors.initials: U
authors.lastname: Rudsander
authors.forename: Tuula T
authors.initials: TT
authors.lastname: Teeri
authors.forename: Daniel
authors.initials: D
authors.lastname: Cullen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002480:Cellulase / Q000235:genetics*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D020075:Phanerochaete / Q000201:enzymology* / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D002480:Cellulase; C451300:cel9A protein, Pseudomonas