Cloning of a gene encoding acetate kinase from Methanosarcina mazei 2-P isolated from a Japanese paddy field soil.
The ack gene encoding acetate kinase from the mesophilic Methanosarcina mazei 2-P, isolated from a paddy field soil in Japan, was cloned, sequenced, and functionally expressed in Escherichia coli. The terminal region of the putative pta gene, probably encoding phosphotransacetylase, was found upstream of the ack gene. The deduced amino acid sequence of the acetate kinase is 86.5% identical to that of the Methanosarcina thermophila acetate kinase. The activity of the His(6)-tagged acetate kinase purified from E. coli JM109 was optimal at 35 degrees C.
Tonouchi
Akio
A
Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Japan. symbio@cc.hirosaki-u.ac.jp
Nishizaki
Yousuke
Y
Tohyama
Hiromi
H
Takeda
Kiyoshi
K
D000084:Acetate Kinase / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007564:Japan; D017020:Methanosarcina / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D000084:Acetate Kinase