G-protein beta-3-subunit and eNOS gene polymorphism in transplant recipients with long-term renal graft function.
BACKGROUND
Despite new immunosuppressive drugs, only a minority of graft survive over 15 years. The aim of our study was to determine the influence of gene polymorphisms in the G-protein-beta(3) subunit (Gbeta3) and endothelial nitric oxide synthase (eNOS) on the long-term outcome of kidney grafts.


METHODS
Using PCR, corresponding genotypes in Gbeta3 (C825T) and eNOS (G894T) gene polymorphism were evaluated in patients with preserved graft function over 15 years and in a control group of transplant recipients.


RESULTS
There were no differences in allele and genotype distributions of both polymorphisms between groups. In Gbeta3 polymorphism, the 825T allele carriers had a significantly lower body mass index while in eNOS polymorphism there were no links between genotypes, renal function and atherosclerosis risk factors.


CONCLUSIONS
Our data suggest that these gene polymorphisms have only a minor influence on long-term renal graft function.
Viklický
Ondrej
O
Department of Nephrology, Institute for Clinical and Experimental Medicine, 1858/9 Videnská, CS-14021 Prague 4, Czech Republic. ondrej.viklicky@medicon.cz
Hubácek
Jaroslav A
JA
Vítko
Stefan
S
Heemann
Uwe W
UW
Vasarhelyi
Barna
B
Kohnle
Mathias
M
Teplan
Vladimir
V
Lácha
Jirí
J
Szabó
Attila J
AJ
D000483:Alleles; D001161:Arteriosclerosis / Q000150:complications; D015992:Body Mass Index; D005260:Female; D005838:Genotype; D006085:Graft Survival / Q000235:genetics* / Q000502:physiology*; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics*; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007677:Kidney Function Tests; D016030:Kidney Transplantation / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III; D011110:Polymorphism, Genetic / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors
C454420:G-protein beta3 subunit; D007166:Immunosuppressive Agents; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; D020962:Heterotrimeric GTP-Binding Proteins