Sodium azide reduces the thermotolerance of respiratively grown yeasts.
The effect of sodium azide in heat shock-induced cell death was studied in Debaryomyces vanrijiae, Candida albicans, and Saccharomyces cerevisiae yeasts. The results presented demonstrate that the azide addition induced a drastic decrease in the thermotolerance of glucose-grown D. vanrijiae. In contrast, glucose-grown S. cerevisiae and C. albicans cells treated with NaN(3) became more resistant to heat shock than control cells. Nevertheless, in galactose medium the decrease of thermotolerance of S. cerevisiae and C. albicans cells was observed in the presence of sodium azide. It was suggested that the decreasing effect of sodium azide on thermotolerance takes place only when the yeast cell is incapable of using fermentation for ATP synthesis and obtains energy via oxidative phosphorylation.
Rikhvanov
Eugene G
EG
Siberian Institute of Plant Physiology and Biochemistry, Siberian Division, Russian Academy of Sciences, Lermontov St. 132, Irkutsk, 664033 Russia. eugene@sifibr.irk.ru
Varakina
Nina N
NN
Rusaleva
Tatyana M
TM
Rachenko
Elena I
EI
Voinikov
Victor K
VK
D003470:Culture Media; D018869:Heat-Shock Response; D010101:Oxygen Consumption / Q000187:drug effects*; D019810:Sodium Azide / Q000494:pharmacology*; D013696:Temperature; D015003:Yeasts / Q000187:drug effects* / Q000254:growth & development* / Q000502:physiology
D003470:Culture Media; D019810:Sodium Azide