Analysis of the role of GADD153 in the control of apoptosis in NS0 myeloma cells.
Apoptosis can limit the maximum production of recombinant protein expression from cultured mammalian cells. This article focuses on the links between nutrient deprivation, ER perturbation, the regulation of (growth arrest and DNA damage inducible gene 153) GADD153 expression and apoptosis. During batch culture, decreases in glucose and glutamine correlated with an increase in apoptotic cells. This event was paralleled by a simultaneous increase in GADD153 expression. The expression of GADD153 in batch culture was suppressed by the addition of nutrients and with fed-batch culture the onset of apoptosis was delayed but not completely prevented. In defined stress conditions, glucose deprivation had the greatest effect on cell death when compared to glutamine deprivation or the addition of tunicamycin (an inhibitor of glycosylation), added to generate endoplasmic reticulum stress. However, the contribution of apoptosis to overall cell death (as judged by morphology) was smaller in conditions of glucose deprivation than in glutamine deprivation or tunicamycin treatment. Transient activation of GADD153 expression was found to occur in response to all stresses and occurred prior to detection of the onset of cell death. These results imply that GADD153 expression is either a trigger for apoptosis or offers a valid indicator of the likelihood of cell death arising from stresses of relevance to the bioreactor environment.
Lengwehasatit
Idsada
I
Biochemistry Research Division, School of Biological Sciences, 2.205 Stopford Building, University of Manchester, Oxford Road, United Kingdom.
Dickson
Alan J
AJ
D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism*; D002452:Cell Count; D002470:Cell Survival; D003470:Culture Media / Q000378:metabolism; D004187:Disaccharides / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D005688:Galactosamine / Q000008:administration & dosage / Q000031:analogs & derivatives*; D005947:Glucose / Q000172:deficiency*; D005973:Glutamine / Q000172:deficiency*; D009101:Multiple Myeloma / Q000473:pathology / Q000503:physiopathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D051742:Transcription Factor CHOP; D014157:Transcription Factors / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D022762:CCAAT-Enhancer-Binding Proteins; D003470:Culture Media; D004187:Disaccharides; D014157:Transcription Factors; D005973:Glutamine; D051742:Transcription Factor CHOP; C076322:tunicamine; D005688:Galactosamine; D005947:Glucose