In vitro detection of apoptosis in monocytes/macrophages infected with human coronavirus.
Human coronavirus (HCoV) strain 229E infection, but not HCoV strain OC43 infection, of monocytes/macrophages from healthy donors and patients with multiple sclerosis in remission resulted in increased apoptosis, as measured by DNA changes and annexin V staining. Apoptosis correlated with the differential release of infectious virus. HCoV strain 229E titers were 10(3.5) to 10(6) 50% tissue culture-infective doses (TCID(50))/ml, and HCoV strain OC43 titers were only 10(1.2) to 10(2.7) TCID(50)/ml.
Collins
Arlene R
AR
Department of Microbiology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, New York 14214, USA. acollins@acsu.buffalo.edu
D000956:Antigens, Viral / Q000032:analysis; D017209:Apoptosis; D002478:Cells, Cultured; D017934:Coronavirus / Q000472:pathogenicity*; D016207:Cytokines / Q000096:biosynthesis; D005434:Flow Cytometry; D006801:Humans; D008264:Macrophages / Q000473:pathology / Q000821:virology*; D009000:Monocytes / Q000473:pathology / Q000821:virology*; D009103:Multiple Sclerosis / Q000821:virology
D000956:Antigens, Viral; D016207:Cytokines