title: Real-time fluorescence-based PCR for detection of malaria parasites.
abstract: A fluorescence-based real-time 5' nuclease PCR capable of detecting all four human malaria parasites was developed to screen large numbers of samples during an outbreak to prevent further transmission of malaria. The effectiveness of antimalarial therapy for malaria patients can be monitored by determining the reduction of parasitemia by this method.
authors.affiliation: Defence Medical Research Institute, Defence Science and Technology Agency, National University of Singapore, Singapore 117597. nmiv13@nus.edu.sg
authors.forename: May-Ann
authors.initials: MA
authors.lastname: Lee
authors.forename: Chiew-Hoon
authors.initials: CH
authors.lastname: Tan
authors.forename: Lay-Tin
authors.initials: LT
authors.lastname: Aw
authors.forename: Choon-Siang
authors.initials: CS
authors.lastname: Tang
authors.forename: Mulkit
authors.initials: M
authors.lastname: Singh
authors.forename: Sze-Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Hwee-Pin
authors.initials: HP
authors.lastname: Chia
authors.forename: Eric P H
authors.initials: EP
authors.lastname: Yap
mesh_terms: D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis; D003851:Deoxyribonucleases / Q000378:metabolism*; D006801:Humans; D008288:Malaria / Q000469:parasitology*; D018512:Parasitemia / Q000469:parasitology; D010961:Plasmodium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D019914:Taq Polymerase / Q000378:metabolism
chemical_list: D016054:DNA, Protozoan; D019914:Taq Polymerase; D003851:Deoxyribonucleases