title: Concentration-dependent behavior of nisin interaction with supported bilayer lipid membrane.
abstract: Nisin is a positively charged antibacterial peptide that binds to the negatively charged membranes of gram-positive bacteria. The initial interaction of the peptide with the model membrane of negatively charged DPPG (dipalmitoylphosphatidylglycerol) was studied by cyclic voltammetry and a.c. impedance spectroscopy. Nisin could induce pores in the supported bilayer lipid membrane, thus, it led to the marker ions Fe(CN)(6)(3-/4-) crossing the lipid membrane and giving the redox reaction on the glassy carbon electrode (GCE). Experimental results suggested that the pore formation on supported bilayer lipid membrane was dependent on the concentration of nisin and it included three main concentration stages: low, middling, high concentration.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China. wmhuang@ciac.jl.cn
authors.forename: Weimin
authors.initials: W
authors.lastname: Huang
authors.forename: Zheling
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Han
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wang
authors.forename: Jilin
authors.initials: J
authors.lastname: Tang
authors.forename: Shaojun
authors.initials: S
authors.lastname: Dong
authors.forename: Erkang
authors.initials: E
authors.lastname: Wang
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry; D017097:Electric Impedance; D004563:Electrochemistry; D007202:Indicators and Reagents; D008051:Lipid Bilayers / Q000737:chemistry*; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D009561:Nisin / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry; D016062:Porosity
chemical_list: D000900:Anti-Bacterial Agents; D003902:Detergents; D007202:Indicators and Reagents; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D010715:Phosphatidylglycerols; D009561:Nisin; C030345:1,2-dipalmitoylphosphatidylglycerol