Aplysia cardioactive peptide (NdWFamide) enhances the L-type Ca2+ current of Aplysia ventricular myocytes.
NdWFamide is a D-amino acid containing tripeptide purified from Aplysia heart. Although the cardioexcitatory action of NdWFamide is well established, little is known about how the excitatory action is induced. To examine the action of the peptide on the ion channels expressed in the Aplysia heart muscles, we carried out whole cell clamp experiments in the isolated Aplysia ventricular myocytes. We found that the high voltage-activated (HVA) Ca(2+) current of Aplysia ventricular myocytes is mostly a nifedipine-sensitive L-type current, and that the current was enhanced by NdWFamide via the activation of G proteins.
Kanemaru
Kazunori
K
Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama 1-3-1, Japan.
Morishita
Fumihiro
F
Matsushima
Osamu
O
Furukawa
Yasuo
Y
D000818:Animals; D001048:Aplysia; D020746:Calcium Channels, L-Type / Q000187:drug effects* / Q000502:physiology; D006352:Heart Ventricles / Q000166:cytology / Q000187:drug effects*; D015640:Ion Channel Gating / Q000187:drug effects; D009842:Oligopeptides / Q000494:pharmacology*
D020746:Calcium Channels, L-Type; D009842:Oligopeptides; C109335:asparaginyl-tryptophyl-phenylalaninamide