28 kDa Fasciola gigantica cysteine proteinase in the diagnosis of prepatent ovine fasciolosis.
Coprological confirmation of ovine fasciolosis in the field, prior to out breaks of the disease and/or strategic antifluke medication, seem to be of little consequence. Efforts are, therefore, being made to evolve a putative antigen specific to serodiagnostic test for early diagnosis during prepatency. In the present investigation, 28 kDa cysteine proteinase was used in ELI SA and Western blot to detect Fasciola gigantica antibodies and further Dipstick-ELISA was developed for field application, using known positive monospecific sera from experimentally infected sheep with 100 F. gigantica metacercariae. Isolation of 28 kDa cysteine proteinase was achieved from bubalian origin flukes. The specific antigen, recognised homologous antifluke antibodies by Western blot as early as 2nd week post-infection (wpi) with 100% sensitivity, in sera samples of sheep harbouring 38 flukes and by 10th wpi in sheep harbouring 3-8 flukes. All sheep were found positive for the infection when ELISA and/or Dipstick-ELISA was applied from 4th wpi. In pooled sera of infected sheep, these were positive during 4th wpi.
Dixit
A K
AK
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243122, India.
Yadav
S C
SC
Sharma
R L
RL
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D015153:Blotting, Western; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005209:Fasciola / Q000201:enzymology* / Q000276:immunology* / Q000302:isolation & purification; D005211:Fascioliasis / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D005260:Female; D008297:Male; D008970:Molecular Weight; D011817:Rabbits; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D012756:Sheep / Q000276:immunology / Q000469:parasitology*; D012757:Sheep Diseases / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D013997:Time Factors
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D003546:Cysteine Endopeptidases