Restoration of ethanol-compromised T(h)1 responses by sodium orthovanadate.
Alcohol consumption often diminishes antigen-specific cell-mediated immunity. In alcohol-consuming mice IFN-gamma and delayed-type hypersensitivity (DTH) responses are blunted, although antigen-specific T cell proliferation and IL-2 responses are largely unaffected, suggesting that alcohol differentially affects signal transduction pathways. In the present report we explore the use of the phosphatase inhibitor, Na3 VO4 to restore IFN-gamma secretion in the presence of ethanol both in vivo and in vitro. We show that Na3 VO4 restores IFN-gamma in vitro and antigen-specific DTH in vivo to the levels seen in alcohol non-consuming mice. Our data support the contention that ethanol, by up-regulating phosphotyrosine phosphatase, diminishes the IFN-gamma signal transduction pathway.
Ostrovidov
Serge
S
Department of Microbiology-Immunology, Northwestern University School of Medicine, 303 East Chicago Avenue, Chicago, IL 60611, USA.
Howard
Laurence M
LM
Ikeda
Masato
M
Ikeda
Akiko
A
Waltenbaugh
Carl
C
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D006967:Hypersensitivity / Q000188:drug therapy / Q000276:immunology; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors; D018417:Th1 Cells / Q000187:drug effects* / Q000276:immunology; D014638:Vanadates / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D000431:Ethanol; D014638:Vanadates; D007371:Interferon-gamma; D017027:Protein Tyrosine Phosphatases