title: Cochlear mitochondrial DNA3867bp deletion in aged mice.
abstract: OBJECTIVE
  To study the status of cochlear mitochondrial DNA (mtDNA) and to determine the location of mtDNA deletion in aged mice.
  
  
  METHODS
  We detected cochlear mtDNA in 2, 7 - 10 and 17 - 19 month old mice by nested polymerase chain reaction (PCR) and DNA sequencing.
  
  
  RESULTS
  mtDNA3867bp deletions were found in the cochleae of aged mice. The deletion occurred within nt9103-nt12970 and were flanked by 15 base pair direct repeats. Comparing the incidence of mtDNA3867bp deletions, 17 - 19 month old mice (7/8) were significantly higher than 7 - 10 month old mice (4/16). The deletion was not observed in 2 month old mice (0/7). The ratio of deleted mtDNA/total mtDNA in 17 - 19 month old mice was higher than in 7 - 10 month old mice (P < 0.001).
  
  
  CONCLUSIONS
  Cochlear mtDNA 3867bp deletion in aged mice may be related to presbycusis.
authors.affiliation: Department of Otolaryngology, PLA General Hospital, Beijing 100853, China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Zhang
authors.forename: Dongyi
authors.initials: D
authors.lastname: Han
authors.forename: Dalian
authors.initials: D
authors.lastname: Ding
authors.forename: Pu
authors.initials: P
authors.lastname: Dai
authors.forename: Weiyan
authors.initials: W
authors.lastname: Yang
authors.forename: Sichang
authors.initials: S
authors.lastname: Jiang
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Salvi
mesh_terms: D000375:Aging / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003051:Cochlea / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D010085:Oxidative Phosphorylation; D011304:Presbycusis / Q000209:etiology*; D017384:Sequence Deletion
chemical_list: D004272:DNA, Mitochondrial