Regulation of freA, acoA, lysF, and cycA expression by iron availability in Aspergillus nidulans.
In the filamentous fungus Aspergillus nidulans, iron homeostasis is regulated at the transcriptional level by the negative-acting GATA factor SREA. In this study the expression of a putative heme-containing metalloreductase-encoding gene, freA, was found to be upregulated by iron limitation independently of SREA, demonstrating the existence of an iron-regulatory mechanism which does not involve SREA. In contrast to freA, various other genes encoding proteins in need of iron-containing cofactors-acoA, lysF, and cycA-were downregulated in response to iron depletion. Remarkably, SREA deficiency led to increased expression of acoA, lysF, and cycA under iron-replete growth conditions.
Oberegger
Harald
H
Department of Molecular Biology. Institute of Legal Medicine, University of Innsbruck, A-6020 Innsbruck, Austria.
Schoeser
Michelle
M
Zadra
Ivo
I
Schrettl
Markus
M
Parson
Walther
W
Haas
Hubertus
H
D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D006836:Hydro-Lyases / Q000096:biosynthesis; D007501:Iron / Q000502:physiology*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D017386:Sequence Homology, Amino Acid
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D007501:Iron; C469336:FreA protein, Aspergillus nidulans; D010088:Oxidoreductases; D006836:Hydro-Lyases; C107855:homoaconitate hydratase