title: Characterization of a novel murine retrovirus mixture that facilitates hematopoiesis.
abstract: A new virus previously arose in BALB/c females mated repeatedly to C57BL/6 (B6) males and then injected with fixed, activated B6 male spleen cells (V. S. Ter-Grigorov, O. Krifuks, E. Liubashevsky, A. Nyska, Z. Trainin, and V. Toder, Nat. Med. 3:37-41, 1997). In the present study, BALB/cJ mice inoculated with virus-containing plasma from affected mice developed splenomegaly, which was caused by increased numbers of Sca-1(+) Lin(-) hematopoietic stem cells (HSC) and their differentiated progeny. Biological and molecular analyses of a new virus revealed a mixture of murine leukemia viruses (MuLVs). These MuLVs comprised ecotropic and mink lung cell focus-forming (MCF) virus classes and are termed Rauscher-like MuLVs because they bear numerous similarities to the ecotropic and MCF viruses of the Rauscher MuLV complex but do not include a spleen focus-forming virus. The ecotropic virus component alone transferred some disease characteristics, while MCF virus alone did not. Thus, we have described a novel virus mixture, termed Rauscher-like MuLV, that causes an increase in hematopoiesis due to activation of pluripotent HSC. Experiments using mice and a protocol that replicated the pregnancy and immunization strategy of the original experiment demonstrated that endogenous BALB/c mouse ecotropic and xenotropic MuLVs are activated by these treatments. Emv1 was expressed in the spleens of multiparous mice but not in those of virgin mice, and Bxv1Emv1-pseudotyped MuLVs were recovered following injection of fixed, activated B6 cells. Thus, multiple pregnancies and allostimuli appear to have provided the signals required for activation of and recombination among endogenous viruses and could have resulted in generation of the Rauscher-like MuLV mixture.
authors.affiliation: The Jackson Laboratory, Bar Harbor, Maine 04609, USA.
authors.forename: Lauren M
authors.initials: LM
authors.lastname: Hook
authors.forename: Brooke A
authors.initials: BA
authors.lastname: Jude
authors.forename: Victor S
authors.initials: VS
authors.lastname: Ter-Grigorov
authors.forename: Janet W
authors.initials: JW
authors.lastname: Hartley
authors.forename: Herbert C
authors.initials: HC
authors.lastname: Morse
authors.forename: Zeev
authors.initials: Z
authors.lastname: Trainin
authors.forename: Vladimir
authors.initials: V
authors.lastname: Toder
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Chervonsky
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Golovkina
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D020077:Endogenous Retroviruses / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity / Q000502:physiology*; D005260:Female; D015752:Genes, env; D006410:Hematopoiesis / Q000502:physiology*; D007519:Isoantigens; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011247:Pregnancy; D011925:Rauscher Virus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology; D012192:Retroviridae Infections / Q000209:etiology; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000821:virology
chemical_list: D007519:Isoantigens