title: Expression of B7 costimulator in eight human malignant hematological cell lines and its significance.
abstract: OBJECTIVE
  To explore the expression of B7 co-stimulator in human malignant hematological cell lines and its significance.
  
  
  METHODS
  Amplified and purified plasmid DNA was transfected by liposome and detection of B7 gene expression by flow cytometry and RT-PCR.
  
  
  RESULTS
  Eight human malignant hematological cell lines U937, K562, CEM, Daudi, GM, PEER, Jurkat, Raji express high level of HLA molecules, low or undetectable level of B7-1 gene, and high level of B7-2 gene. Expression level of B7-1 gene was enhanced after B7-1 transfection. T cell expressed high level of IL-2 mRNA after B7-1 transfection.
  
  
  CONCLUSIONS
  Multiple human malignant hematological cell lines express low or undetectable level of B7-1 gene, suggesting that B7-1 plays a critical role in tumor immunity.
authors.affiliation: The Third Hospital, Beijing University, Beijing 100083, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Jia
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ke
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
mesh_terms: D018122:B7-1 Antigen; D002460:Cell Line; D005434:Flow Cytometry; D006801:Humans; D007376:Interleukin-2; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection; D020298:U937 Cells
chemical_list: D018122:B7-1 Antigen; D007376:Interleukin-2