Localization of a new highly repeated DNA sequence of Lemur cafta (Lemuridae, Strepsirhini).
We have isolated and cloned an 800-bp highly repeated DNA (HRDNA) sequence from Lemur catta (LCA) and described its localization on LCA chromosomes. Lemur catta HRDNA sequences were localized by performing FISH experiments on standard and elongated metaphasic chromosomes using an LCA HRDNA probe (LCASAT). A complex hybridization pattern was detected. A strong pericentromeric hybridization signal was observed on most LCA chromosomes. Chromosomes 7 and 13 were lit in pericentromeric regions, as well as in the interspersed heterochromatin. Chromosomes 1, 3, 4, 17, 19, X, and microchromosomes (20, 25, 26, and 27) showed no signals in the pericentromeric region, but chromosomes 3 and 4 showed a positive hybridization in heterochromatic regions. The 800-bp L catta HRDNA was species specific. We performed FISH experiments with the LCASAT probe on Eulemur macaco macaco (EMA) and Eulemur fulvus fulvus (EFU) metaphases and no positive signal of hybridization was detected. These findings were also confirmed by Southern blot analysis and PCR.
Boniotto
Michele
M
Università di Trieste, Dipartimento di Scienze della Riproduzione e dello Sviluppo, Italy.
Ventura
Mario
M
Cardone
Maria Francesca
MF
Boaretto
Francesca
F
Archidiacono
Nicoletta
N
Rocchi
Mariano
M
Crovella
Sergio
S
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D006570:Heterochromatin / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D007900:Lemur / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D017931:DNA Primers; D006570:Heterochromatin; D004247:DNA