Surface modification of polycaprolactone membrane via aminolysis and biomacromolecule immobilization for promoting cytocompatibility of human endothelial cells.
Amino groups were covalently introduced onto a polycaprolactone (PCL) surface by the reaction between 1,6-hexanediamine and the ester groups of PCL. The occurrence of the aminolysis and the introduction of free NH(2) groups were verified qualitatively by fluorescence spectroscopy, where rhodamine B isothiocyanate was employed to label NH(2) groups, and quantitatively by absorbance spectroscopy, where ninhydrin was used to react with NH(2) to generate a blue product. Due to the presence of deep pores on the PCL membrane, the aminolysis reaction could penetrate as deep as 50 microm to yield NH(2) density as high as 2 x 10(-7) mol/cm(2). By use of the NH(2) groups as active sites, biocompatible macromolecules such as gelatin, chitosan, or collagen were further immobilized on the aminolyzed PCL membrane via a cross-linking agent, glutaraldehyde. X-ray photoelectron spectroscopy (XPS) and surface wettability measurements confirmed the coupling of the biomacromolecules. The endothelial cell culture proved that the cytocompatibility of the aminolyzed PCL was improved slightly regardless of the NH(2) amount on the surface. After immobilization of the biomacromolecules, however, the cell attachment and proliferation ratios were obviously improved and the cells showed a similar morphology to those on tissue culture polystyrene. Measurement of the von Willebrand factor (vWF) secreted by these endothelial cells (ECs) verified the endothelial function. Hence, a better EC-compatible PCL was produced.
Zhu
Yabin
Y
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Gao
Changyou
C
Liu
Xingyu
X
Shen
Jiacong
J
D000586:Amination; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001704:Biopolymers / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D002455:Cell Division / Q000187:drug effects; D004727:Endothelium / Q000166:cytology*; D006801:Humans; D008567:Membranes, Artificial; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods; D014841:von Willebrand Factor / Q000378:metabolism
D001672:Biocompatible Materials; D001704:Biopolymers; D008567:Membranes, Artificial; D011091:Polyesters; D014841:von Willebrand Factor; C016240:polycaprolactone