Intraspecific variability and pharmacokinetic characteristics of Androctonus mauretanicus mauretanicus scorpion venom.
We evaluated the degree of venom toxicity and protein content of several specimens of Androctonus mauretanicus mauretanicus. The quantity of protein of individual venom obtained after manual extraction from 31 different scorpions varied from a minimum of 0.15 mg to a maximum of 1.53 mg. We determined the venom toxicity, in mice, by estimating the number of LD(50)s of 20 scorpions chosen randomly among the 31 scorpions. It ranged from less than 40 LD(50)s to a maximum of 272 LD(50)s. The correlation between protein content and venom lethality is not systematic. We also determined the pharmacokinetics of the venom and its specific anti-venom in rabbits to compare their distribution and elimination properties. After a subcutaneous injection, high concentrations of venom were measured by ELISA in the vascular space rapidly after the injection (T(max) = 0.5 h). The terminal half-life was 2.8 h, close the one determined after intravenous injection (t(1/2beta) = 3.2 h). The total volume of distribution (Vd(ss) or Vd(beta)) was between 317 and 380 ml/kg. The total body clearance was 82 ml/kg/h. For scorpion anti-venom, the terminal half-life, after intravenous injection, was 20.25 h; the volume of distribution was 83 ml/kg and the total body clearance was 3 ml/kg/h. After intramuscular administration, T(max) was reached at 36 h. The results show that venom lethality varies from specimen to specimen and that pharmacokinetic parameters of venom and anti-venom are totally different. This must be taken under consideration in anti-venom production (anti-venom titre) as well as in therapeutic protocols (dose, injection route) to improve serotherapy.
El Hafny
B
B
Unité des Venins et Toxines, Département de Recherche, Institut Pasteur du Maroc, 1 Place Abou Kacem Ez-zahraoui, Casablanca, Morocco. bouchra.elhafny@pasteur.ma
Chgoury
F
F
Adil
N
N
Cohen
N
N
Hassar
M
M
D000818:Animals; D000997:Antivenins / Q000008:administration & dosage* / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D006736:Horses; D007273:Injections, Intramuscular; D007275:Injections, Intravenous; D007279:Injections, Subcutaneous; D007928:Lethal Dose 50; D051379:Mice; D008815:Mice, Inbred Strains; D011817:Rabbits; D011971:Receptors, Immunologic / Q000276:immunology; D012604:Scorpion Venoms / Q000008:administration & dosage / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics* / Q000633:toxicity*; D012605:Scorpions
D000997:Antivenins; C052643:F(ab')2 receptor; D011971:Receptors, Immunologic; D012604:Scorpion Venoms