Plo1(+) regulates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle.
The regulation of gene expression plays an important part in cell cycle controls. We describe the molecular machinery that co-ordinates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle. A sequence is identified in the cdc15(+) promoter that we call a PCB (pombe cell cycle box), which confers M-G(1)-specific transcription. Sequences similar to the PCB are present in the promoters of seven other genes, spo12(+), cdc19(+), fin1(+), sid2(+), ppb1(+), mid1(+)/dmf1(+) and plo1(+), which we find to be transcribed at M-G(1). A transcription factor complex is identified that binds to the PCB sequence, which we name PBF, for PCB-binding factor. Finally, we show that PBF binding activity and consequent gene transcription are regulated by the Plo1p protein kinase, thus invoking a potential auto-feedback loop mechanism that regulates mitotic gene transcription and passage through septation and cytokinesis.
Anderson
Mark
M
Division of Biochemistry and Molecular Biology, Institute of Biological and Life Sciences, University of Glasgow, UK.
Ng
Szu Shien
SS
Marchesi
Vanessa
V
MacIver
Fiona H
FH
Stevens
Frances E
FE
Riddell
Tracy
T
Glover
David M
DM
Hagan
Iain M
IM
McInerny
Christopher J
CJ
D001483:Base Sequence; D017931:DNA Primers; D016193:G1 Phase; D005800:Genes, Fungal / Q000502:physiology*; D008938:Mitosis / Q000502:physiology*; D012568:Schizosaccharomyces / Q000166:cytology*; D014158:Transcription, Genetic / Q000502:physiology*
D017931:DNA Primers