Activator recruitment by the general transcription machinery: X-ray structural analysis of the Oct-1 POU domain/human U1 octamer/SNAP190 peptide ternary complex.
Transcriptional activation of the human U1 snRNA genes is dependent on a noncanonical octamer element contained within an upstream enhancer. The U1 octamer only weakly recruits the Oct-1 POU domain, although recruitment is stimulated by a peptide containing the Oct-1-binding domain of SNAP190. Structural analysis of the Oct-1 POU domain/U1 octamer/SNAP190 peptide complex revealed that SNAP190 makes extensive protein contacts with the Oct-1 POU-specific domain and with the DNA phosphate backbone within the enhancer. Although SNAP190 and OCA-B both interact with the Oct-1 POU domain through the same Oct-1 interface, a single nucleotide within the U1 octamer ablates OCA-B recruitment without compromising activator recruitment by SNAP190.
Hovde
Stacy
S
Department of Chemistry, Michigan State University, East Lansing, Michigan 48823, USA.
Hinkley
Craig S
CS
Strong
Katie
K
Brooks
Aimee
A
Gu
Liping
L
Henry
R William
RW
Geiger
James
J
D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D051863:Host Cell Factor C1; D006801:Humans; D009690:Nucleic Acid Conformation; D050811:Octamer Transcription Factor-1; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary / Q000235:genetics; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D015533:Transcriptional Activation
D004268:DNA-Binding Proteins; C497692:HCFC1 protein, human; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; C494165:POU2F1 protein, human; D012342:RNA, Small Nuclear; C116351:SNAPC4 protein, human; D014157:Transcription Factors; C405483:U1 small nuclear RNA