Gene delivery into primary T cells: overview and characterization of a transgenic model for efficient adenoviral transduction.
Technologies for transfer of exogenous genes into primary T cells have been limited until recently. The introduction of new approaches for gene transfer via different viral vectors has expanded the options for genetic manipulation of primary T cells and has provided powerful tools for studies of T cell activation and differentiation. We provide a brief overview of the systems currently available and contrast the advantages and disadvantages of each. We also describe a new transgenic model that enables highly efficient gene delivery into primary T cells by nonreplicating adenoviral vectors.
Hurez
Vincent
V
Department of Pathology, University of Alabama at Birmingham, 35233-2170, USA.
Hautton
Robin D
RD
Oliver
James
J
Matthews
R James
RJ
Weaver
Casey K
CK
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D015704:CD4 Antigens / Q000235:genetics; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D000229:Dependovirus / Q000235:genetics; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D011991:Receptors, Virus / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology*; D014161:Transduction, Genetic
D015704:CD4 Antigens; C568771:CLMP protein, human; C568772:CLMP protein, mouse; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D011991:Receptors, Virus