Increased expression of B-lymphocyte chemoattractant, but not pro-inflammatory cytokines, in muscle tissue in rhesus chronic Lyme borreliosis.
Inflammation in skeletal muscle is a consistent feature of Lyme borreliosis, both in the human disease and experimental models. This study had two goals: to evaluate the expression of selected pro-inflammatory and chemokine genes in skeletal muscle in the Rhesus model of Lyme disease, and to identify unexpected cytokine genes involved in Lyme myositis. Two different techniques for measuring cytokine messenger RNA (mRNA) levels were used to achieve these goals: gene expression microarrays and. real-time RT-PCR (Taqman). Muscle from necropsies and biopsies were used, and were obtained from both infected and uninfected non-human primates (NHPs). Although many cytokines were found expressed in muscle tissue, pro-inflammatory cytokines commonly associated with inflammation were not consistently upregulated in infected muscles relative to uninfected muscles. However, B-lymphocyte chemoattractant (BLC), a chemokine implicated in the trafficking of B-cells into tissue, was increased in expression. This study is the first to extensively characterize cytokine gene expression in chronically inflamed tissue in Lyme borreliosis.
Pachner
Andrew R
AR
Department of Neurosciences, University of Medicine and Dentistry of New Jersey--New Jersey Medical School, Newark, NJ 07103, USA.
Dail
Donna
D
Narayan
Kavitha
K
Dutta
Kaberi
K
Cadavid
Diego
D
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D025065:Borrelia burgdorferi / Q000302:isolation & purification*; D054382:Chemokine CXCL13; D019743:Chemokines, CXC / Q000096:biosynthesis*; D002908:Chronic Disease; D016207:Cytokines / Q000096:biosynthesis*; D020869:Gene Expression Profiling / Q000379:methods; D018836:Inflammation Mediators / Q000378:metabolism; D008193:Lyme Disease / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008253:Macaca mulatta; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
C110663:CXCL13 protein, human; D054382:Chemokine CXCL13; D019743:Chemokines, CXC; D016207:Cytokines; D018836:Inflammation Mediators