[Effects of selenium and fluoride on apoptosis and lipid peroxidation in human hepatocytes].
OBJECTIVE
To study the influence of selenium and fluoride on apoptosis and lipid peroxidation in human hepatocytes in vitro.


METHODS
The apoptosis, cell cycle, GSH content and lipid peroxides (LPO) level in human hepatocytes, LPO level and LDH, AST and ALT activity in cell culture supernatants were investigated after hepatocytes were incubated with selenium and/or fluoride for around 12 hours periods in vitro.


RESULTS
The percentage of hepatocyte apoptosis bodies (15.557 +/- 2.056)%, the number of cells in S phase (4.823 +/- 0.454)% and LPO level in liver tissue and supernatant [(2.884 +/- 0.589) and (3.547 +/- 0.561) nmol/L MDA/mg.prot, respectively], AST and LDH activity in supernatants (91.1 +/- 36.4 and 140.4 +/- 7.6 U/L, respectively) in the fluoride treated group was higher than the control group [(10.313 +/- 1.023)%, (3.253 +/- 0.743)%, (1.473 +/- 0.401) nmol/L MDA/mg.prot, (1.694 +/- 0.443) nmol/L MDA/mg.prot, (54.5 +/- 3.2) U/L and (126.4 +/- 2.6) U/L, respectively], The GSH content in live tissue [(4.225 +/- 0.781) microgram/mg.prot] is lower than control group [(7.595 +/- 1.042) microgram/mg.prot]. Selenium treatment reduced these kinds of toxicity of fluoride through raising GSH content, reducing LPO level, LDH and AST activity and percentage of apoptosis bodies.


CONCLUSIONS
Selenium can antagonist apoptosis and lipid peroxidation of hepatocytes induced by fluoride.
Wang
Aiguo
A
Department of Environmental Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
Xia
Tao
T
Ran
Peng
P
Bai
Yun
Y
Yang
Kedi
K
Chen
Xuemin
X
D000410:Alanine Transaminase / Q000187:drug effects / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D001219:Aspartate Aminotransferases / Q000187:drug effects / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D005459:Fluorides / Q000494:pharmacology*; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008054:Lipid Peroxides / Q000378:metabolism; D012643:Selenium / Q000494:pharmacology*; D013997:Time Factors
D008054:Lipid Peroxides; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; D012643:Selenium; D005459:Fluorides