Babesia divergens: cloning and biochemical characterization of Bd37.
The immunoprotective potential of Babesia divergens antigens released in supernatants of in vitro cultures of the parasite is generally known. Among a number of parasite molecules, a 37 kDa protein has been found in the supernatants of Babesia divergens cultures. In this report the cloning and biochemical characterization of this protein, called Bd37, are described. In addition, the processing of the protein was studied in vitro. Results suggest that Bd37 is encoded by a single copy gene. Bd37 appears to be a merozoite-associated molecule attached to the surface by a glycosylphosphatidylinositol moiety containing a palmitate residue attached to the inositol ring. In addition, it is demonstrated that both extremities of the protein are linked by a disulphide bond. Results further indicate that a soluble, hydrophilic form of Bd37 can be released from the merozoite surface by GPI-specific phospholipase D. The potential role the Bd37 protein and the GPI anchor are discussed.
Delbecq
S
S
Laboratoire de Biologie Cellulaire et Moleculaire, Faculté de Pharmacie, Université Montpellier I, France.
Precigout
E
E
Vallet
A
A
Carcy
B
B
Schetters
T P M
TP
Gorenflot
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001403:Babesia / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004220:Disulfides; D005260:Female; D015870:Gene Expression; D015723:Gene Library; D017125:Genes, Protozoan / Q000235:genetics; D005849:Gerbillinae; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals; D014357:Trypsin / Q000378:metabolism
D000953:Antigens, Protozoan; D004220:Disulfides; D008565:Membrane Proteins; D021382:Protein Sorting Signals; D014357:Trypsin