A rapid and efficient method using chromoslots to assign any newly cloned DNA sequence to its cognate chromosome in the filamentous fungus Podospora anserina.
An efficient method was developed to assign cloned genes to individual chromosomes of the fungus Podospora anserina. The chromosomes were separated by pulsed-field gel electrophoresis and the DNA was isolated from the gel bands. The DNA from the isolated chromosomes was slotted onto membranes; the resulting chromoslots were used to confirm that genetically mapped genes could be detected in the expected position. Then, 20 genes, not yet assigned to a linkage group, were attributed to individual chromosomes while six were attributed to a band containing two chromosomes.
Barreau
Christian
C
Laboratoire de Biologie et Génomique de Podospora, Institut de Biochimie et Génétique Cellulaires, UMR CNRS 5095, Université de Bordeaux 2, France. christian.barreau@ibgc.u-bordeaux2.fr
Sellem
Carole
C
Silar
Philippe
P
Sainsard-Chanet
Annie
A
Turcq
Béatrice
B
D001203:Ascomycota / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D005800:Genes, Fungal; D008040:Genetic Linkage
D004269:DNA, Bacterial