Chemical approaches untangling sequence-specific DNA binding by proteins.
Structure-based design of novel DNA-binding proteins provides an ultimate test of our understanding of protein-DNA interactions. A combination of synthetic, organic, biochemical and molecular biological approaches has been developed to study the principle of molecular recognition associated with the protein-DNA interactions. The strategies enabled a specific formation of noncovalent peptide dimers and determination of the preferential DNA-binding sequence of short peptides.
Sato
Shin-ichi
S
Institute of Advanced Energy, Kyoto University Uji, Kyoto 611-0011, Japan.
Hagihara
Masaki
M
Sugimoto
Kenji
K
Morii
Takashi
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D019281:Dimerization; D015195:Drug Design; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D013379:Substrate Specificity
D004268:DNA-Binding Proteins; D004247:DNA