In vitro assay of hydrolysis and chlorohydroxy derivatives of bisphenol A diglycidyl ether for estrogenic activity.
Bisphenol A diglycidyl ether (BADGE) is an epoxy resin monomer. Epoxy-based solution coatings are used in many applications as additives for a variety of plastic coatings in food packaging. It is well known that unreacted BADGE can migrate from epoxy-based packing materials into foods. Not only BADGE but also its derivatives can easily migrate into foods and it is likely that we intake BADGE and its derivatives through food or drink. Recently, endocrine disrupting chemicals (EDCs) have attracted attention because they have been shown to affect reproduction in wildlife. The estrogenic activity of BADGE derivatives has not previously been investigated. Therefore, we investigated the estrogenic activity of the BADGE derivatives, dihydrolysed BADGE (BADGE-4OH) and chlorohydroxy BADGE (BADGE-2Cl), using breast cancer cell (T47D) proliferation assay and estrogen receptor (ER) (alpha) binding assay. These chemicals exhibited T47D cell proliferation at concentrations of 10(-14)-10(-4) M. However, these chemicals did not bind to ER (alpha) in the binding assay.
Nakazawa
H
H
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo 142-8501, Japan. nakazawa@hoshi.ac.jp
Yamaguchi
A
A
Inoue
K
K
Yamazaki
T
T
Kato
K
K
Yoshimura
Y
Y
Makino
T
T
D001559:Benzhydryl Compounds; D001943:Breast Neoplasms; D002273:Carcinogens / Q000378:metabolism / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004852:Epoxy Compounds / Q000378:metabolism / Q000633:toxicity*; D047628:Estrogen Receptor alpha; D005260:Female; D005506:Food Contamination; D018857:Food Packaging; D006801:Humans; D006868:Hydrolysis; D011960:Receptors, Estrogen / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D002273:Carcinogens; D004852:Epoxy Compounds; D047628:Estrogen Receptor alpha; D011960:Receptors, Estrogen; C019273:bisphenol A diglycidyl ether; D000098826:Bisphenol A Compounds