Cloning, expression and characterization of a mammalian Nudix hydrolase-like enzyme that cleaves the pyrophosphate bond of UDP-glucose.
A distinct UDP-glucose (UDPG) pyrophosphatase (UGPPase, EC 3.6.1.45) has been characterized using pig kidney ( Sus scrofa ). This enzyme hydrolyses UDPG, the precursor molecule of numerous glycosylation reactions in animals, to produce glucose 1-phosphate (G1P) and UMP. Sequence analyses of the purified enzyme revealed that, similar to the case of a nucleotide-sugar hydrolase controlling the intracellular levels of ADP-glucose linked to glycogen biosynthesis in Escherichia coli [Moreno-Bruna, Baroja-Fernández, Muñoz, Bastarrica-Berasategui, Zandueta-Criado, Rodri;guez-López, Lasa, Akazawa and Pozueta-Romero (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 8128-8132], UGPPase appears to be a member of the ubiquitously distributed group of nucleotide pyrophosphatases designated Nudix hydrolases. A complete cDNA of the UGPPase-encoding gene, designated UGPP, was isolated from a human thyroid cDNA library and expressed in E. coli. The resulting cells accumulated a protein that showed kinetic properties identical to those of pig UGPPase.
Yagi
Toshihiro
T
JCR Pharmaceuticals Co. Ltd., Pharmacological Science, Research Division, 2-2-10 Murotani, Nishi-ku, Kobe 651-2241, Japan.
Baroja-Fernández
Edurne
E
Yamamoto
Ryuji
R
Muñoz
Francisco José
FJ
Akazawa
Takashi
T
Hong
Kyoung Su
KS
Pozueta-Romero
Javier
J
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D005943:Gluconeogenesis / Q000502:physiology; D006801:Humans; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D011755:Pyrophosphatases / Q000235:genetics* / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D013379:Substrate Specificity; D034421:Sus scrofa
D011755:Pyrophosphatases; C023255:UDPglucose pyrophosphatase