title: Fertilization, embryonic development and pregnancy losses with intracytoplasmic sperm injection for surgically-retrieved spermatozoa.
abstract: Prior to the development of intracytoplasmic sperm injection (ICSI), azoospermic and severely oligozoospermic men had little to no chance of having a biological child. In this study, ICSI outcome in 454 transfers with ejaculated spermatozoa and 59 transfers with surgically retrieved spermatozoa were evaluated. Normal fertilization rate using ejaculated spermatozoa was 75% of 5995 oocytes, and 73% of 751 oocytes for surgically retrieved spermatozoa; with ongoing pregnancy rates of 53% (242/454) and 61% (36/59) respectively. Surgically retrieved spermatozoa significantly (P < 0.05) impacted 1PN oocytes (6.1%, 46/751), severely fragmented embryos (8.8%, 46/550) and incidence of pregnancy loss (11%, 4/36). When using ejaculated spermatozoa, incidence of 1PN oocytes, severely fragmented embryos and pregnancy loss was 2.9% (177/5995), 4.5% (200/4365), 2.4% (6/242) respectively.
authors.affiliation: 200 Hawthorne Lane, 6A-IVF, Charlotte, NC 28233, USA. Tranderson@novanthealth.org
authors.forename: Anthony R
authors.initials: AR
authors.lastname: Anderson
authors.forename: Klaus E
authors.initials: KE
authors.lastname: Wiemer
authors.forename: Margaret L
authors.initials: ML
authors.lastname: Weikert
authors.forename: Melissa L
authors.initials: ML
authors.lastname: Kyslinger
mesh_terms: D000022:Abortion, Spontaneous / Q000453:epidemiology*; D001755:Blastocyst / Q000166:cytology / Q000502:physiology; D010064:Embryo Implantation; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D011247:Pregnancy; D011255:Pregnancy Maintenance / Q000502:physiology; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*