title: Intron status and 3'-end formation control cotranscriptional export of mRNA.
abstract: Messenger RNA export factors are recruited to genes in a transcription-dependent manner. To ascertain the mechanism of this process, we show that RNA polymerase II transcription is sufficient to recruit the Saccharomyces cerevisiae hnRNP protein Npl3 to a gene independent of RNA sequence. In contrast, the cotranscriptional recruitment of the RNA-binding protein Yra1 is dependent on pre-mRNA processing. Yra1 associates with introns of intron-containing genes in a splicing-dependent manner. Conversely, Yra1 recruitment to genes without introns is not dependent on splicing. Finally, 3'-end formation is required for Yra1 recruitment to genes regardless of intron status.
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
authors.forename: Elissa P
authors.initials: EP
authors.lastname: Lei
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Silver
mesh_terms: D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000235:genetics; D007438:Introns / Q000235:genetics*; D008958:Models, Molecular; D009687:Nuclear Proteins / Q000235:genetics; D026721:RNA 3' End Processing / Q000235:genetics; D012326:RNA Splicing; D012331:RNA, Fungal / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D034441:Heterogeneous-Nuclear Ribonucleoproteins; D009687:Nuclear Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; C105887:YRA1 protein, S cerevisiae