title: Registration of serial tissue section images.
abstract: OBJECTIVE: This study aims to tackle the problem of image registration during computer-assisted three-dimensional (3D) reconstruction of serial tissue sections. METHODS: We proposed segmentation-counting algorithm for computerized image registration on the basis of joint histogram. This approach utilizes thresholding of the 2 images to be registered, and the criterion function is defined as the counting in a specific region of the joint histogram. The registration parameters can be obtained by optimizing the criterion function. RESULTS: In the trial application of this approach in image registration for the serial tissue sections of mouse wse embryos, a more efficient result was achieved. CONCLUSION: The method can rapidly accomplish the image registration task for serial tissue sections with simpler calculation processes.
authors.affiliation: Key Lab for Medical Images, First Military Medical University, Guangzhou 510515, China.
authors.forename: Zhe-Xing
authors.initials: ZX
authors.lastname: Liu
authors.forename: Wu
authors.initials: W
authors.lastname: Dong
authors.forename: Shu-Xiang
authors.initials: SX
authors.lastname: Li
authors.forename: Gui-Ping
authors.initials: GP
authors.lastname: Jiang
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiao-Mian
authors.initials: XM
authors.lastname: Xie