Production of manganese-dependent peroxidase in a new solid-state bioreactor by Phanerochaete chrysosporium grown on wood shavings. Application to the decolorization of synthetic dyes.
The production of manganese-dependent peroxidase (MnP) by Phanerochaete chrysosporium in a new solid-state bioreactor, the immersion bioreactor, operating with lignocellulosic waste, such as wood shavings, was investigated. Maximum MnP and lignin peroxidase (LiP) activity of 13.4 and 8.48 mukat/L were obtained, respectively. The in vitro decolorization of several synthetic dyes by the extracellular liquid produced in the above-mentioned bioreactor (containing mainly MnP) was carried out and its degrading ability was assessed. The highest decolorization was reached with Indigo Carmine (98%) followed by Bromophenol Blue (56%) and Methyl Orange (36%), whereas Gentian Violet was hardly decolorized (6%).
Rodríguez Couto
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Department of Chemical Engineering, University of Vigo, Lagoas Marcosende, 36200 Vigo, Spain.
Domínguez
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Sanromán
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D001673:Biodegradation, Environmental; D019149:Bioreactors; D003116:Color; D004396:Coloring Agents / Q000378:metabolism; D003470:Culture Media; D010544:Peroxidases / Q000096:biosynthesis* / Q000378:metabolism; D020075:Phanerochaete / Q000201:enzymology* / Q000254:growth & development; D013053:Spectrophotometry; D014934:Wood
D004396:Coloring Agents; D003470:Culture Media; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase