title: Plzf mediates transcriptional repression of HoxD gene expression through chromatin remodeling.
abstract: The molecular mechanisms that regulate coordinated and colinear activation of Hox gene expression in space and time remain poorly understood. Here we demonstrate that Plzf regulates the spatial expression of the AbdB HoxD gene complex by binding to regulatory elements required for restricted Hox gene expression and can recruit histone deacetylases to these sites. We show by scanning forced microscopy that Plzf, via homodimerization, can form DNA loops and bridge distant Plzf binding sites located within HoxD gene regulatory elements. Furthermore, we demonstrate that Plzf physically interacts with Polycomb proteins on DNA. We propose a model by which the balance between activating morphogenic signals and transcriptional repressors such as Plzf establishes proper Hox gene expression boundaries in the limb bud.
authors.affiliation: Molecular Biology Program, Department of Pathology, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Barna
authors.forename: Taha
authors.initials: T
authors.lastname: Merghoub
authors.forename: José A
authors.initials: JA
authors.lastname: Costoya
authors.forename: Davide
authors.initials: D
authors.lastname: Ruggero
authors.forename: Matthew
authors.initials: M
authors.lastname: Branford
authors.forename: Anna
authors.initials: A
authors.lastname: Bergia
authors.forename: Bruno
authors.initials: B
authors.lastname: Samori
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Pandolfi
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006657:Histones / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics*; D051741:Kruppel-Like Transcription Factors; D051379:Mice; D018625:Microscopy, Atomic Force; D063146:Polycomb-Group Proteins; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D012097:Repressor Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D006657:Histones; D018398:Homeodomain Proteins; D051741:Kruppel-Like Transcription Factors; D063146:Polycomb-Group Proteins; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D012097:Repressor Proteins; D014157:Transcription Factors; C513267:Zbtb16 protein, mouse; D004247:DNA