Serological differentiation of infections with dengue virus serotypes 1 to 4 by using recombinant antigens.
The B domains of dengue virus serotypes 1 to 4 were expressed in Escherichia coli. The purified proteins were applied to immunoblot strips to detect serotype-specific antibodies in paired serum samples from 41 patients with primary and secondary dengue infections. A close correlation between the results obtained with the immunoblot strips and by type-specific reverse transcription-PCR (T. Laue, P. Emmerich, and H. Schmitz, J. Clin. Microbiol. 37:2543-2547, 1999) was observed.
Ludolfs
Diana
D
Bernhard-Nocht Institute for Tropical Medicine, Hamburg, Germany.
Schilling
Stefan
S
Altenschmidt
Jan
J
Schmitz
Herbert
H
D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology*; D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting; D011994:Recombinant Proteins / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012703:Serotyping
D000914:Antibodies, Viral; D000956:Antigens, Viral; D011994:Recombinant Proteins