Cultivation of primmorphs from the marine sponge Suberites domuncula: morphogenetic potential of silicon and iron.
Marine demosponges (phylum Porifera) are rich sources for potent bioactive compounds. With the establishment of the primmorph system from sponges, especially from Suberites domuncula, the technology to cultivate sponge cells in vitro improved considerably. This progress was possible after the elucidation that sponges are provided with characteristic metazoan cell adhesion receptors and extracellular matrix molecules which allow their cells a positioning in a complex organization pattern. This review summarizes recent data on the cultivation of sponges in aquaria and--with main emphasis--of primmorphs in vitro. It is outlined that silicon and Fe(+++) contribute substantially to the formation of larger primmorphs (size of 10 mm) as well as of a canal system in primmorphs; canals are probably required for an improved oxygen and food supply. We conclude that the primmorph system will facilitate a sustainable use of sponges in the production of bioactive compounds; it may furthermore allow new and hitherto not feasible insights into basic questions on the origin of Metazoa.
Le Pennec
Gaël
G
Institut für Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Universität, Duesbergweg 6, D-55099 Mainz, Germany.
Perovic
Sanja
S
Ammar
Mohammed Shokry A
MS
Grebenjuk
Vladislav A
VA
Steffen
Renate
R
Brümmer
Franz
F
Müller
Werner E G
WE
D000818:Animals; D019149:Bioreactors; D002449:Cell Aggregation / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D017753:Ecosystem; D005293:Ferritins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006133:Growth Substances / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology; D009024:Morphogenesis / Q000502:physiology; D011161:Porifera / Q000145:classification / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development*; D017640:Silicates / Q000494:pharmacology; D013045:Species Specificity
D006133:Growth Substances; D017640:Silicates; D005293:Ferritins; D007501:Iron