Adhesion properties of human bladder cell lines with extracellular matrix components: the role of integrins and glycosylation.
Integrin subunits present on human bladder cells displayed heterogeneous functional specificity in adhesion to extracellular matrix proteins (ECM). The non-malignant cell line (HCV29) showed significantly higher adhesion efficiency to collagen IV, laminin (LN) and fibronectin (FN) than cancer (T24, Hu456) and v-raf transfected (BC3726) cell lines. Specific antibodies to the alpha(2), alpha(5) and beta(1) integrin subunits inhibited adhesion of the non-malignant cells, indicating these integrin participation in the adhesion to ECM proteins. In contrast, adhesion of cancer cells was not inhibited by specific antibodies to the beta(1) integrin subunit. Antibodies to alpha(3) integrin increased adhesion of cancer cells to collagen, LN and FN, but also of the HCV29 line with collagen. It seems that alpha(3) subunit plays a major role in modulation of other integrin receptors especially in cancer cells. Differences in adhesion to ECM proteins between the non-malignant and cancer cell lines in response to Gal and Fuc were not evident, except for the v-raf transfected cell line which showed a distinct about 6-fold increased adhesion to LN on addition of both saccharides. N-Acetylneuraminic acid inhibited adhesion of all cell lines to LN and FN irrespective of their malignancy.
Lityńska
Anna
A
Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Kraków, Poland. Lita@zuk.iz.uj.edu.pl
Przybyło
Malgorzta
M
Pocheć
Ewa
E
Laidler
Piotr
P
D000911:Antibodies, Monoclonal / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology*; D002461:Cell Line, Transformed; D003094:Collagen / Q000145:classification / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism / Q000502:physiology*; D005353:Fibronectins / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D016023:Integrins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D007797:Laminin / Q000378:metabolism; D009005:Monosaccharides / Q000737:chemistry / Q000494:pharmacology; D027384:Oncogene Proteins v-raf / Q000378:metabolism; D021122:Protein Subunits; D014162:Transfection; D014407:Tumor Cells, Cultured; D001743:Urinary Bladder / Q000166:cytology* / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology; D019459:Urothelium / Q000166:cytology
D000911:Antibodies, Monoclonal; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D016023:Integrins; D007797:Laminin; D009005:Monosaccharides; D021122:Protein Subunits; D003094:Collagen; D027384:Oncogene Proteins v-raf