title: Changes in peripheral blood lymphocyte subsets in elderly subjects are associated with an impaired function of the hypothalamic-pituitary-adrenal axis.
abstract: A growing body of evidence indicates that ageing brings a progressive disruption in the immune and endocrine systems. However, very few reports have correlated the changes in the immune system with the endocrine function in the elderly. The aim of the present study was to investigate the changes occurring in the peripheral blood lymphocyte subpopulations with age and correlate them with the hypothalamic-pituitary-adrenal (HPA) function. We determined the peripheral blood lymphocyte phenotype and the T cell receptor usage by flow cytometry analysis. The HPA function was evaluated by the basal serum levels of adrenal steroids and the response to stimulation with a low-dose ACTH. In the elderly, we observed a decrease of major T subsets together with an increase of NK cells and activated T cells. With regard to the HPA function, the most significant decline was found in dehydroepiandrosterone (DHEA) and dehydroepiandrosterone-sulphate (DHEAS). A close correlation between immune changes with ageing and DHEA response to ACTH stimulation was found. The present study showed an inverse correlation of lymphocyte changes with the plasma levels of steroids, especially DHEA and its metabolite, DHEAS. This association was not found for other steroids and points for the possibility of using DHEA to correct the immunological decline associated with ageing.
authors.affiliation: Services of Rheumatology, Immunology, and Endocrinology, Santander, Spain. inmlhm@humv.es
authors.forename: Victor
authors.initials: V
authors.lastname: Martínez-Taboada
authors.forename: María José
authors.initials: MJ
authors.lastname: Bartolomé
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Amado
authors.forename: Ricardo
authors.initials: R
authors.lastname: Blanco
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: García-Unzueta
authors.forename: Vicente
authors.initials: V
authors.lastname: Rodríguez-Valverde
authors.forename: Marcos
authors.initials: M
authors.lastname: López-Hoyos
mesh_terms: D000305:Adrenal Cortex Hormones / Q000097:blood; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D000735:Androstenedione / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism; D003366:Cosyntropin; D003687:Dehydroepiandrosterone / Q000097:blood; D005260:Female; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007030:Hypothalamo-Hypophyseal System / Q000503:physiopathology*; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010913:Pituitary-Adrenal System / Q000503:physiopathology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D012016:Reference Values
chemical_list: D000305:Adrenal Cortex Hormones; D006684:HLA-DR Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D015375:Receptors, Interleukin-2; D003366:Cosyntropin; D000735:Androstenedione; D003687:Dehydroepiandrosterone; D006854:Hydrocortisone