Inhibition of polyomavirus ori-dependent DNA replication by mSin3B.
When tethered in cis to DNA, the transcriptional corepressor mSin3B inhibits polyomavirus (Py) ori-dependent DNA replication in vivo. Histone deacetylases (HDACs) appear not to be involved, since tethering class I and class II HDACs in cis does not inhibit replication and treating the cells with trichostatin A does not specifically relieve inhibition by mSin3B. However, the mSin3B L59P mutation that impairs mSin3B interaction with N-CoR/SMRT abrogates inhibition of replication, suggesting the involvement of N-CoR/SMRT. Py large T antigen interacts with mSin3B, suggesting an HDAC-independent mechanism by which mSin3B inhibits DNA replication.
Xie
An-Yong
AY
Department of Biochemistry, University of Missouri-Columbia, Columbia, Missouri 65211, USA.
Folk
William R
WR
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D004261:DNA Replication / Q000235:genetics*; D004279:DNA, Viral / Q000096:biosynthesis* / Q000235:genetics*; D006655:Histone Deacetylases / Q000378:metabolism; D051379:Mice; D008954:Models, Biological; D011120:Polyomavirus / Q000235:genetics* / Q000378:metabolism*; D018741:Replication Origin; D012097:Repressor Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
D004279:DNA, Viral; D012097:Repressor Proteins; C115812:Sin3b protein, mouse; D014157:Transcription Factors; D006655:Histone Deacetylases