Hammett analysis of a C-C hydrolase-catalysed reaction using synthetic 6-aryl-2-hydroxy-6-ketohexa-2,4-dienoic acid substrates.
A Hammett plot (rho = -0.71) has been measured for C-C hydrolase enzyme BphD from Pseudomonas LB400, using six 6-aryl-2-hydroxy-6-ketohexa-2,4-dienoic acids synthesised by a Heck coupling strategy.
Speare
Damian M
DM
Department of Chemistry, University of Warwick, Coventry, UK CV4 7AL.
Olf
Petra
P
Bugg
Timothy D H
TD
D000466:Alkadienes / Q000138:chemical synthesis / Q000378:metabolism; D002396:Catechols / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism*; D007651:Keto Acids / Q000138:chemical synthesis / Q000378:metabolism*; D007700:Kinetics; D011549:Pseudomonas / Q000201:enzymology; D013379:Substrate Specificity
D000466:Alkadienes; D002396:Catechols; D007651:Keto Acids; C024269:2-hydroxy-6-oxonanoic acid; D006867:Hydrolases; C062907:2,6-dioxo-6-phenylhexa-3-enoate hydrolase