title: In vitro calcium phosphate growth over surface modified PMMA film.
abstract: In vitro nucleation of calcium phosphate phase was studied over functionalized polymethyl methacrylate (PMMA) films using Fourier transform infrared spectroscopy, electron spectroscopy, scanning electron microscopy and energy dispersive X-ray analysis. PMMA films were prepared by dissolving commercial grade pellets in chloroform and cast into thin sheets. The films were immersed in a methanol solution of sodium hydroxide before treating with 1.5% solution of adenosine triphosphate (ATP) at a pH of 5.2 for 24 h. ATP treated films were then soaked in saturated lime solution for 4 days to initiate formation of calcium phosphate precursor phase over their surface. The above films immersed in simulated body fluid solution (1.5 x SBF) for more than 5 days led to the nucleation of apatitic calcium phosphate phase all over the film surface. The ATP coupled film not subjected to lime treatment did not show calcium phosphate nucleation behaviour upon immersion in SBF solution. The Ca/P ratio of the calcium phosphate phase increase with increase in soaking time in SBF solution.
authors.affiliation: Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Thiruvananthapuram 695 012, India.
authors.forename: H K
authors.initials: HK
authors.lastname: Varma
authors.forename: K
authors.initials: K
authors.lastname: Sreenivasan
authors.forename: Y
authors.initials: Y
authors.lastname: Yokogawa
authors.forename: A
authors.initials: A
authors.lastname: Hosumi
mesh_terms: D002130:Calcium Phosphates / Q000737:chemistry*; D004577:Electron Probe Microanalysis; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; D019904:Polymethyl Methacrylate; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous