Large-scale production of coenzyme F420-5,6 by using Mycobacterium smegmatis.
Production of coenzyme F420 and its biosynthetic precursor FO was examined with a variety of aerobic actinomycetes to identify an improved source for these materials. Based on fermentation costs, safety, and ease of growth, Mycobacterium smegmatis was the best source for F420-5,6. M. smegmatis produced 1 to 3 micromol of intracellular F420 per liter of culture, which was more than the 0.85 to 1.0 micromol of F420-2 per liter usually obtained with Methanobacterium thermoautotrophicum and approximately 10-fold higher than what was previously reported for the best aerobic actinomycetes. An improved chromatography system using rapidly flowing quaternary aminoethyl ion-exchange material and Florisil was used to more quickly and easily purify F420 than with previous methods.
Isabelle
Dale
D
Department of Microbiology, University of Iowa, Iowa City, Iowa 52242, USA.
Simpson
D Randall
DR
Daniels
Lacy
L
D039903:Actinobacteria / Q000254:growth & development / Q000378:metabolism; D000332:Aerobiosis; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D020102:Mycobacterium smegmatis / Q000378:metabolism*; D012256:Riboflavin / Q000031:analogs & derivatives* / Q000096:biosynthesis*
C015084:coenzyme F420; D012256:Riboflavin