cDNA cloning, sequencing, and differential expression of a heart-type fatty acid-binding protein in the mummichog (Fundulus heteroclitus).
A heart fatty acid-binding protein (H-FABP) cDNA was isolated from the liver of the mummichog (Fundulus heteroclitus) and the complete cDNA sequence and predicted amino acid sequence was determined. This cDNA binds to a mRNA product of 0.95 kb with the highest level of RNA expression in the male liver, gills, and gonads. Exposure of mummichogs to the polycyclic aromatic hydrocarbon (PAH) pyrene significantly down-regulated H-FABP expression at the highest concentration. This study provides evidence that environmentally relevant toxicants can modulate H-FABP expression.
Bain
Lisa J
LJ
Department of Biological Sciences, University of Texas at El Paso, 79968, USA. lbain@utep.edu
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004781:Environmental Exposure; D050556:Fatty Acid-Binding Proteins; D023781:Fundulidae / Q000502:physiology*; D005786:Gene Expression Regulation; D005880:Gills / Q000737:chemistry; D006066:Gonads / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects*; D012313:RNA / Q000096:biosynthesis
D002352:Carrier Proteins; D018076:DNA, Complementary; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D011084:Polycyclic Aromatic Hydrocarbons; D012313:RNA