Continuous solid-phase synthesis and disulfide cyclization of peptide-PNA-peptide chimeras.
Chelator peptides were extended from the N-terminus of peptide nucleic acid (PNA) dodecamers, which in turn were extended from the N-termini of disulfide-bridged peptide ligand analogues, using Fmoc coupling for all residues. The cysteine thiols were cyclized on a solid support, either before or after PNA extension. This simplified synthetic approach might allow preparation of a variety of multipeptide disulfide-bridged PNA chimeras. [structure: see text]
Tian
Xiaobing
X
Departments of Biochemistry & Molecular Pharmacology and Microbiology & Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Wickstrom
Eric
E
D000595:Amino Acid Sequence; D001483:Base Sequence; D004220:Disulfides / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000138:chemical synthesis*
D004220:Disulfides; D020135:Peptide Nucleic Acids; D010455:Peptides; D010456:Peptides, Cyclic