Protective immunity of single and multi-antigen DNA vaccines against schistosomiasis.
We evaluated the usefulness of the combination of three plasmids encoding tegumental (pECL and pSM14) and muscular (pIRV5) antigens of the Schistosoma mansoni on improving the protective immunity over the use of a single antigen as DNA vaccines. Female BALB/c mice were inoculated twice with 25 micro g DNA plasmid within two weeks interval. The challenge was performed with 80 cercarias of a regional isolate of S. mansoni (SLM) one week after the last immunization. Six weeks after challenge, all mice were perfused for worm load determination. The following groups were analyzed: saline; empty vector; monovalent formulations of pECL; pSM14 and pIRV5 and also double combinations of pECL/pIRV5 and pIRV5/pSM14 and a triple combination of pECL/pIRV5/pSM14. The protection was expressed as a percentage of worm loads in each group compared with the saline group. The results obtained were 41% (p < 0.05); 52% (p < 0.05); 51% (p < 0.05); 48% (p < 0.05); 55% (p < 0.05); 45% (p < 0.05); 65% (p < 0.05) for each group respectively.
Nascimento
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E
Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fiocruz, Recife, PE, 50670-420, Brasil.
Leão
I C
IC
Pereira
V R A
VR
Gomes
Y M
YM
Chikhlikar
P
P
August
T
T
Marques
E
E
Lucena-Silva
N
N
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D002352:Carrier Proteins; D050612:Fatty Acid Transport Proteins; D005260:Female; D015801:Helminth Proteins / Q000276:immunology; D026901:Membrane Transport Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000276:immunology*; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000517:prevention & control*; D017778:Vaccines, Combined / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*
D000909:Antibodies, Helminth; D002352:Carrier Proteins; D050612:Fatty Acid Transport Proteins; D015801:Helminth Proteins; D026901:Membrane Transport Proteins; D017778:Vaccines, Combined; D019444:Vaccines, DNA; C068709:SM14 protein, Schistosoma mansoni