SycE allows secretion of YopE-DHFR hybrids by the Yersinia enterocolitica type III Ysc system.
The Ysc type III secretion system allows Yersinia enterocolitica to translocate virulence proteins, called Yop effectors, into the cytosol of eukaryotic cells. Some of the Yop effectors possess an individual chaperone called a Syc protein. The first 15 amino acids of the YopE effector constitute a secretion signal that is sufficient to promote secretion of several reporter proteins. Residues 15-50 of YopE comprise the minimal binding domain for the SycE chaperone. In this study, we investigated the secretion by the Ysc system of several YopE-DHFR hybrid proteins with different folding properties, and evaluated the role of SycE, the cognate chaperone of YopE, in this context. We have analysed the secretion of hybrids containing 16 (YopE16), 52 (YopE52) and 80 (the complete region covered by the chaperone, YopE80) amino acids of YopE or full-length YopE (YopEFL) with wild-type DHFR and two mutants with altered folding properties. The hybrids containing DHFR delta77, the mutant whose folding properties are the most drastically affected, could be secreted in all the conditions tested, even in the absence of the chaperone SycE. In contrast, DHFRwt could only be secreted fused to the first 52 amino acids of YopE, and its secretion was strictly dependent on SycE. The hybrids YopE80-DHFRwt and YopEFL-DHFRwt were not secreted. YopEFL-DHFRwt completely jammed the channel in an SycE-dependent fashion. Our experiments indicate that, in order to be secreted, proteins must be unfolded or only partially folded, and that TSS chaperones could keep their substrates in a secretion-competent conformation, probably by preventing their folding. In addition, they show that the secretion apparatus can reject folded proteins if they are not deeply engaged into the injectisome.
Feldman
Mario F
MF
Division of Molecular Microbiology, University of Basel, Switzerland.
Müller
Simone
S
Wüest
Esther
E
Cornelis
Guy R
GR
D000630:Aminopterin / Q000494:pharmacology; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009154:Mutation; D017510:Protein Folding; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D015008:Yersinia enterocolitica / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; C081269:SycE protein, Yersinia; D015534:Trans-Activators; C063239:yopE protein, Yersinia; D013762:Tetrahydrofolate Dehydrogenase; D000630:Aminopterin