Characterization of a novel human UDP-GalNAc transferase, pp-GalNAc-T10.
A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) gene family was cloned as a homolog of human pp-GalNAc-T7, and designated pp-GalNAc-T10. pp-GalNAc-T10 transcript was found in the small intestine, stomach, pancreas, ovary, thyroid gland and spleen. In a polypeptide GalNAc-transfer assay, recombinant pp-GalNAc-T10 transferred GalNAc onto a panel of mucin-derived peptide substrates. Furthermore, pp-GalNAc-T10 demonstrated strong transferase activity with glycopeptide substrates.
Cheng
Lamei
L
Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Central-2, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan.
Tachibana
Kouichi
K
Zhang
Yan
Y
Guo
Jian ming
Jm
Kahori Tachibana
Kahori
K
Kameyama
Akihiko
A
Wang
Han
H
Hiruma
Toru
T
Iwasaki
Hiroko
H
Togayachi
Akira
A
Kudo
Takashi
T
Narimatsu
Hisashi
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics* / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000096:biosynthesis; D013379:Substrate Specificity; D014018:Tissue Distribution; D000097763:Polypeptide N-acetylgalactosaminyltransferase
D017350:N-Acetylgalactosaminyltransferases; D010455:Peptides; D012333:RNA, Messenger; D000097763:Polypeptide N-acetylgalactosaminyltransferase