title: A rapid and efficient method using chromoslots to assign any newly cloned DNA sequence to its cognate chromosome in the filamentous fungus Podospora anserina.
abstract: An efficient method was developed to assign cloned genes to individual chromosomes of the fungus Podospora anserina. The chromosomes were separated by pulsed-field gel electrophoresis and the DNA was isolated from the gel bands. The DNA from the isolated chromosomes was slotted onto membranes; the resulting chromoslots were used to confirm that genetically mapped genes could be detected in the expected position. Then, 20 genes, not yet assigned to a linkage group, were attributed to individual chromosomes while six were attributed to a band containing two chromosomes.
authors.affiliation: Laboratoire de Biologie et Génomique de Podospora, Institut de Biochimie et Génétique Cellulaires, UMR CNRS 5095, Université de Bordeaux 2, France. christian.barreau@ibgc.u-bordeaux2.fr
authors.forename: Christian
authors.initials: C
authors.lastname: Barreau
authors.forename: Carole
authors.initials: C
authors.lastname: Sellem
authors.forename: Philippe
authors.initials: P
authors.lastname: Silar
authors.forename: Annie
authors.initials: A
authors.lastname: Sainsard-Chanet
authors.forename: Béatrice
authors.initials: B
authors.lastname: Turcq
mesh_terms: D001203:Ascomycota / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D005800:Genes, Fungal; D008040:Genetic Linkage
chemical_list: D004269:DNA, Bacterial