Photodynamic therapy with photofrin reduces invasiveness of malignant human glioma cells.
In this study we investigated the influence of Photofrin-based photodynamic therapy (PDT) on the migration of two human glioma cell lines in vitro. U87 and U25ln tumour cells were treated with Photofrin at various doses and subjected to a fixed optical (632 nm) dose of 100 mJ/cm(2). Photofrin cytotoxicity was determined using MTT and colony forming assays. Using a matrigel artificial basement membrane migration assay, we demonstrated that low doses of subcytotoxic PDT treatment, such as PDT with 2.5 micro g/ml Photofrin on U87 cells and 1 micro g/ml on U25ln cells, significantly ( p<0.001) inhibited in vitro migration of both cell lines. Furthermore, in a qualitative spheroid confrontation assay, subcytotoxic PDT of co-cultures between tumour spheroids and brain aggregates resulted in an absence of progressive tumour invasion and destruction of the brain aggregate. In conclusion, our data indicate that low-dose subcytotoxic PDT with Photofrin significantly inhibits invasiveness of U87 and U25ln cells.
Jiang
F
F
Henry Ford Health Sciences Center, Department of Neurology, Detroit, MI, USA.
Chopp
M
M
Katakowski
M
M
Cho
K-K
KK
Yang
X
X
Hochbaum
N
N
Tong
L
L
Mikkelsen
T
T
D000818:Animals; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000473:pathology; D018920:Coculture Techniques; D017323:Dihematoporphyrin Ether / Q000008:administration & dosage*; D005910:Glioma / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D018613:Microscopy, Confocal; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D051381:Rats; D018874:Spheroids, Cellular / Q000187:drug effects; D014407:Tumor Cells, Cultured
D017319:Photosensitizing Agents; D017323:Dihematoporphyrin Ether