[Screening and analysis of early cardiopathology-related gene in type 2 diabetes mellitus].
OBJECTIVE
To screen and analyze early cardiopathology related gene in type 2 diabetes mellitus (DM).


METHODS
(1) To develop a rat model of type 2 DM, SD rats were injected with streptozotocin (STZ, 15 mg/kg) after high caloric diet had been given for two months. (2) After establishment of the model for half a month, one and a half month and six months respectively, dissected myocardium was observed under electron microscope. (3) After establishment of the model for half a month, myocardium was analyzed with fluorescence labelled mRNA differential display. Positive clones were BLAST after conformed by Northern blot. Via the application of bioinformatics, the function and structure of unknown sequences were predicted.


RESULTS
(1) The model of type 2 DM developed with the character of lightly impaired islet function and insulin resistance. (2) Diabetic cardiomyopathy was verified by the ultrastructural change of myocardium which became serious with time. (3) Screened and conformed clones were muscle carnitine palmitoyltransferase 1 and 8 and novel sequences were named DCM1, 2, 5, 6, 8, 11, 12, 16, which had been submitted to Genbank. DCM 5 was predicted to play a role of catalysis or signal conduction via the application of bioinformatics.


CONCLUSIONS
The altered mRNA expression of these genes suggested that they were candidates for a role in the development of cardiopathy prior to the ultrastructural alterations of myocardium. The change of key enzyme in energy metabolism may be the initial factor during heart functional disorder. Further investigation was required for reverse intervention of diabetic cardiopathy in early period.
Zhang
Fanglin
F
Shanghai Institute of Endrocrinology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China. fanglin@yahoo.com
Li
Guo
G
Ding
Wei
W
Liu
Youping
Y
Xie
Chao
C
Zhang
Di
D
Zhou
Wenzhong
W
Sun
Weihua
W
Yang
Jialin
J
Luo
Min
M
D000818:Animals; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000473:pathology; D015870:Gene Expression; D008297:Male; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D012333:RNA, Messenger