title: Mechanics of F-actin characterized with microfabricated cantilevers.
abstract: In this report we characterized the longitudinal elasticity of single actin filaments manipulated by novel silicon-nitride microfabricated levers. Single actin filaments were stretched from zero tension to maximal physiological tension, P(0). The obtained length-tension relation was nonlinear in the low-tension range (0-50 pN) with a resultant strain of approximately 0.4-0.6% and then became linear at moderate to high tensions (approximately 50-230 pN). In this region, the stretching stiffness of a single rhodamine-phalloidin-labeled, 1-microm-long F-actin is 34.5 +/- 3.5 pN/nm. Such a length-tension relation could be characterized by an entropic-enthalpic worm-like chain model, which ascribes most of the energy consumed in the nonlinear portion to overcoming thermal undulations arising from the filament's interaction with surrounding solution and the linear portion to the intrinsic stretching elasticity. By fitting the experimental data with such a worm-like chain model, an estimation of persistence length of approximately 8.75 microm was derived. These results suggest that F-actin is more compliant than previously thought and that thin filament compliance may account for a substantial fraction of the sarcomere's elasticity.
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, WA 98195 USA.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Liu
authors.forename: Gerald H
authors.initials: GH
authors.lastname: Pollack
mesh_terms: D000199:Actins / Q000737:chemistry*; D000818:Animals; D001703:Biophysics / Q000295:instrumentation / Q000379:methods; D002645:Chickens; D019277:Entropy; D006358:Hot Temperature; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D018715:Microscopy, Video / Q000379:methods; D009991:Oscillometry; D010590:Phalloidine / Q000494:pharmacology; D012235:Rhodamines / Q000494:pharmacology; D012518:Sarcomeres / Q000737:chemistry; D017655:Silicon Compounds / Q000737:chemistry; D013696:Temperature; D013816:Thermodynamics
chemical_list: D000199:Actins; D012235:Rhodamines; D017655:Silicon Compounds; D010590:Phalloidine; C032734:silicon nitride