Hydrophilic and lipophilic antioxidant activities of grapes.
The 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)/H2O2/horseradish peroxidase (ABTS/H2O2/HRP) decoloration method permits the evaluation of the antioxidant activity of complex food samples. This method is capable of determining both hydrophilic (in buffered media) and lipophilic (in organic media) antioxidant properties in the same sample. Its application to the study of the antioxidant properties of grapes, one white and two black, has permitted us to establish important differences in the antioxidant activity. The black grapes showed higher antioxidant activity than the white variety. The determination of hydrophilic antioxidant activity (HAA) and lipophilic antioxidant activity (LAA) in the three grapes showed that a minor contribution (4-13%) of total antioxidant activity (TAA) was due to LAA in all cases. The experimentally determined HAA could be approximated using the relative contributions of the calculated values of reference compounds (total phenols as gallic acid and anthocyanins as malvidin).
Alcolea
Jesús F
JF
Departamento de Biología Vegetal (Fisiología Vegetal), Facultad de Biología, Campus Espinardo, Universidad de Murcia, E-30100 Murcia, Spain.
Cano
Antonio
A
Acosta
Manuel
M
Arnao
Marino B
MB
D000872:Anthocyanins / Q000032:analysis; D000975:Antioxidants / Q000032:analysis*; D052160:Benzothiazoles; D003116:Color; D006735:Horseradish Peroxidase; D007202:Indicators and Reagents; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis; D012997:Solvents; D013053:Spectrophotometry; D013451:Sulfonic Acids; D027843:Vitis / Q000737:chemistry* / Q000145:classification
D000872:Anthocyanins; D000975:Antioxidants; D052160:Benzothiazoles; D007202:Indicators and Reagents; D010636:Phenols; D012997:Solvents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D006735:Horseradish Peroxidase