Cloning and characterization of a novel synaptosome-enriched mRNA that encodes 31 kDa protein.
Although a subpopulation of mRNAs has been identified as translocated to the dendrites or the synaptic regions of neurons, the translocational mechanism has not been elucidated. To find mRNAs enriched in synapses, we compared the synaptosomal mRNAs with those from whole forebrain using differential display (DD). We cloned one of these mRNAs, which encoded a novel 31 kDa protein (PMES-2). PMES-2 mRNA was specifically transcribed in the brain and was present in the dendrites of the hippocampal neurons. PMES-2 protein was partly localized in the postsynaptic density. Although this protein is very similar to human NABC1 protein, its function is still unknown.
Ishimoto
Tetsuya
T
Neuronics Research Group, Special Division for Human Life Technology, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31, Midorigaoka, Ikeda, Japan.
Ninomiya
Kensuke
K
Miyaji
Kazue
K
Uyeda
Atsuko
A
Kasai
Michiki
M
Taguchi
Takahisa
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D003712:Dendrites / Q000378:metabolism; D004926:Escherichia coli; D020869:Gene Expression Profiling; D015151:Immunoblotting; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D016133:Polymerase Chain Reaction; D016548:Prosencephalon / Q000378:metabolism*; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016415:Sequence Alignment; D013574:Synaptosomes / Q000378:metabolism*
C113699:BCAS1 protein, human; C470839:Bcas1 protein, rat; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D012313:RNA