title: Quantitative analysis of osteonectin mRNA in thyroid carcinomas.
abstract: Our recent study of the gene expression profile in thyroid carcinoma showed an overexpression of osteonectin mRNA, an extracellular matrix protein, in an anaplastic carcinoma. To confirm this, we measured the expression levels of osteonectin mRNA in 84 thyroid normal and tumor tissues, including five anaplastic carcinomas by realtime quantitative reverse-transcription PCR. Increased expression of osteonectin mRNA was observed in anaplastic carcinoma tissue. However, in five anaplastic carcinoma cell lines, no increase was observed in the expression levels of osteonectin mRNA. These findings suggest the possibility that increased expression of osteonectin mRNA in anaplastic carcinoma tissue may be due to its overexpression in stromal cells, but not in anaplastic carcinoma cells.
authors.affiliation: Department of Laboratory Medicine, Osaka University Medical School, Suita, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Takano
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Hasegawa
authors.forename: Akira
authors.initials: A
authors.lastname: Miyauchi
authors.forename: Fumio
authors.initials: F
authors.lastname: Matsuzuka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Kanji
authors.initials: K
authors.lastname: Kuma
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Hayashi
authors.forename: Shoji
authors.initials: S
authors.lastname: Nakamori
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Amino
mesh_terms: D002277:Carcinoma / Q000737:chemistry*; D003199:Computer Systems; D006801:Humans; D015676:Osteonectin / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013961:Thyroid Gland / Q000737:chemistry; D013964:Thyroid Neoplasms / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D015676:Osteonectin; D012333:RNA, Messenger