title: Arsenate reductases in prokaryotes and eukaryotes.
abstract: The ubiquity of arsenic in the environment has led to the evolution of enzymes for arsenic detoxification. An initial step in arsenic metabolism is the enzymatic reduction of arsenate [As(V)] to arsenite [As(III)]. At least three families of arsenate reductase enzymes have arisen, apparently by convergent evolution. The properties of two of these are described here. The first is the prokaryotic ArsC arsenate reductase of Escherichia coli. The second, Acr2p of Saccharomyces cerevisiae, is the only identified eukaryotic arsenate reductase. Although unrelated to each other, both enzymes receive their reducing equivalents from glutaredoxin and reduced glutathione. The structure of the bacterial ArsC has been solved at 1.65 A. As predicted from its biochemical properties, ArsC structures with covalent enzyme-arsenic intermediates that include either As(V) or As(III) were observed. The yeast Acr2p has an active site motif HC(X)(5)R that is conserved in protein phosphotyrosine phosphatases and rhodanases, suggesting that these three groups of enzymes may have evolved from an ancestral oxyanion-binding protein.
authors.affiliation: Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, 540 East Canfield Avenue, Detroit, MI 48201, USA. rmukhopad@med.wayne.edu
authors.forename: Rita
authors.initials: R
authors.lastname: Mukhopadhyay
authors.forename: Barry P
authors.initials: BP
authors.lastname: Rosen
mesh_terms: D053502:Arsenate Reductases; D001149:Arsenates / Q000494:pharmacology; D001151:Arsenic / Q000378:metabolism*; D053501:Arsenite Transporting ATPases; D004351:Drug Resistance; D004926:Escherichia coli / Q000201:enzymology*; D016623:Ion Pumps / Q000494:pharmacology*; D009097:Multienzyme Complexes / Q000494:pharmacology*; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D001149:Arsenates; D016623:Ion Pumps; D009097:Multienzyme Complexes; D029701:Saccharomyces cerevisiae Proteins; C506971:ARR2 protein, S cerevisiae; D053502:Arsenate Reductases; D053501:Arsenite Transporting ATPases; D001151:Arsenic