title: Detection of a novel pattern of connexin 43 immunoreactivity responsive to dehydration in rat hypothalamic magnocellular nuclei.
abstract: Immunocytochemical expression of Connexin 43 (Cx 43) in the rat Supraoptic Nucleus was analyzed following dehydration, using sequence-specific anti-Cx 43 antibodies (designated 13-8300, 71-0700, and sc-9059) that exhibit differential recognition of Cx 43. Punctate and longitudinally arranged immunostaining patterns of Cx 43 labeling, as evidenced by antibody sc-9059, was detected overlaying the nucleus of magnocellular neuroendocrine cells. This novel form of longitudinally arranged Cx 43 immunoreactivity was modified by dehydration and halothane exposure, but not lactation.
authors.affiliation: Unidad de Neurobiologia Aplicada (CEMIC-CONICET), Av. Galván 4102, 1431, Buenos Aires, Argentina.
authors.forename: Ines
authors.initials: I
authors.lastname: Eisner
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Colombo
mesh_terms: D000818:Animals; D018031:Connexin 43 / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology*; D003681:Dehydration / Q000276:immunology / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D007774:Lactation / Q000502:physiology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013495:Supraoptic Nucleus / Q000737:chemistry* / Q000378:metabolism
chemical_list: D018031:Connexin 43