Cationic liposome-mediated gene delivery in vivo.
Several improvements have been made in liposomal delivery, thus making this technology potentially useful for treatment of certain diseases in the clinic. Success in non-viral delivery is complicated and requires optimization of several components. These components include nucleic acid purification, plasmid design, formulation of the delivery vehicle, administration route and schedule, dosing, detection of gene expression, and others. With further improvements, broad use of non-viral delivery systems to treat human disorders should be possible.
Templeton
Nancy Smyth
NS
Baylor College of Medicine, Center for Cell and Gene Therapy, Houston, TX 77030, USA. NANCY@bcm.tmc.edu
D000818:Animals; D002412:Cations / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D002478:Cells, Cultured; D004274:DNA, Recombinant / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics / Q000493:pharmacokinetics; D006207:Half-Life; D006801:Humans; D008081:Liposomes / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D046911:Macromolecular Substances; D055672:Static Electricity; D013499:Surface Properties; D014018:Tissue Distribution; D014780:Viruses / Q000235:genetics
D002412:Cations; D004274:DNA, Recombinant; D008081:Liposomes; D046911:Macromolecular Substances