title: Ocular surface inflammation induced by Propionibacterium acnes.
abstract: OBJECTIVE
  In the eye, is commonly isolated in the lid, conjunctiva, and meibomian gland secretion. Well known as a causative bacterium of granulomatous endophthalmitis and a potent inflammatory stimulus, reportedly induces a delayed-type hypersensitivity (DTH) response and forms granulomas in the liver and lung in animal models. In this study, we examined whether can induce a DTH response in the cornea.
  
  
  METHODS
  Six- to 8-week-old female Lewis rats were immunized with heat-killed suspension of and assessed as to DTH response via ear challenge at 2 weeks after immunization. At 3 weeks after immunization, suspension was injected in the rat corneal stroma, which was then observed biomicroscopically at 6, 24, and 48 hours after injection. Phenol-killed suspension was also used for the comparison. Histological examination was also performed on the corneal tissues, using hematoxylin and eosin staining as well as immunohistochemical staining against CD4 and CD8 T cells.
  
  
  RESULTS
  Rats immunized with suspension showed significantly higher ear swelling values at both the 24- and 48-hour measurements than did the naïve controls (p < 0.005). Massive cellular infiltration with stromal edema was observed biomicroscopically at 48 hours after injection of suspension in the corneal stroma. Histological study showed that the cell infiltration pattern was similar to that of DTH in the skin, i.e., neutrophils infiltrated at 6 hours, followed by mononuclear cells that, including macrophages and lymphocytes, increased and mixed with neutrophils, accompanied by stromal edema at 48 hours. Immunohistochemical study revealed that CD4 T-cell infiltration in the corneal stroma appeared to predominate over CD8 T-cell infiltration.
  
  
  CONCLUSIONS
  These results indicate that can induce a DTH response in the cornea and may be a causative bacterium of ocular surface inflammation.
authors.affiliation: Department of Ophthalmology, Kyoto Prefectural University of Medicine Kajii-cho, Kawaramachi-Hirokoji Kamigyo-ku, Kyoto City 602-0841, Japan. tsuzuki@ophth.kpu-m.ac.jp
authors.forename: Tomo
authors.initials: T
authors.lastname: Suzuki
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Sano
authors.forename: Osamu
authors.initials: O
authors.lastname: Sasaki
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kinoshita
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003319:Corneal Stroma / Q000382:microbiology; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D007634:Keratitis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D023421:Models, Animal; D011425:Propionibacterium acnes / Q000502:physiology*; D051381:Rats; D011917:Rats, Inbred Lew