title: New variations in intron 4 of growth hormone gene in Chinese native chickens.
abstract: Polymorphism in intron 4 of chicken growth hormone (cGH) gene was studied in 20 Chinese native chicken populations and broiler or layer populations. A total of eight restriction digestion profiles were identified in intron 4 and confirmed by sequencing. Among 20 populations, there were distinctively different allele numbers and frequencies of intron 4 restriction fragment length polymorphisms (RFLPs) between Chinese native chickens and broilers or layers. Two new alleles, allele D and allele E, were identified in Taihe Silkies. Allele D was also identified in other Chinese native breeds and a 50 bp fragment deletion was identified in allele E.
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Nie
authors.forename: S C Y
authors.initials: SC
authors.lastname: Ip
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: F C
authors.initials: FC
authors.lastname: Leung
authors.forename: G
authors.initials: G
authors.lastname: Yang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D017931:DNA Primers; D013006:Growth Hormone / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D013006:Growth Hormone