title: Pyrococcus furiosus ferredoxin is a functional dimer.
abstract: Pyrococcus furiosus ferredoxin is subject to a monomer/dimer equilibrium as a function of ionic strength. At physiological ionic strength, approximately 0.35 M NaCl, the protein is very predominantly homodimer. The monomeric form exhibits impaired electron transfer on glassy carbon; it also has a decreased S=3/2 over S=1/2 ratio as shown by electron paramagnetic resonance spectroscopy. Even following sterilization at 121 degrees C the dimer is stable in denaturing gel electrophoresis.
authors.affiliation: Kluyver Department of Biotechnology, Delft University of Technology, Delft, The Netherlands. m.n.hasan@tnw.tudelft.nl
authors.forename: M N
authors.initials: MN
authors.lastname: Hasan
authors.forename: P L
authors.initials: PL
authors.lastname: Hagedoorn
authors.forename: W R
authors.initials: WR
authors.lastname: Hagen
mesh_terms: D002850:Chromatography, Gel; D019281:Dimerization; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport; D004591:Electrophoresis, Polyacrylamide Gel; D005288:Ferredoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology*; D009994:Osmolar Concentration; D020027:Pyrococcus furiosus
chemical_list: D005288:Ferredoxins