title: Blazeispirane and protoblazeispirane derivatives from the cultured mycelia of the fungus Agaricus blazei.
abstract: Two blazeispirane derivatives including blazeispirols G and I were isolated from the cultured mycelia of the fungus Agaricus blazei Murill and were established to be (20S, 22S, 23R, 24S)-14 beta,22: 22,25-diepoxy-5-methoxy-des-A-ergosta-5,7,9-triene-11 alpha,23-diol and (20S, 22S, 23R, 24S)-14 beta,22:22,25-diepoxy-5-methoxy-des-A-ergosta-5,7,9,11-tetraene-23,28-diol by comparison of extensive 1D and 2D NMR spectral data with that of blazeispirol A. Furthermore, four blazeispirol derivatives blazeispirols, U, V, V(1) and Z(1) were isolated form the same source described above. Their structures were determined to be (20S, 22S, 23R, 24S)-14 beta,22:22,25-diepoxy-23-hydroxyergosta-4,6,8,11-tetraen-3-one, (20S, 22S, 23R, 24S)-14 beta,22:22,25-diepoxy-6 alpha,7 alpha,23-trihydroxyergosta-4,8,11-trien-3-one, (20S, 22S, 23R, 24S)-14 beta,22:22,25-diepoxy-6 beta,7 alpha,23-trihydroxyergosta-4,8,11-trien-3-one and (20S, 22S, 23R, 24S)-14 beta,22:22,25-diepoxy-23-hydroxy-4,5-seco-ergosta-6,8-diene-3,5-dione by extensive 1 D and 2D NMR spectral data.
authors.affiliation: School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan. hirotanim@pharm.kitasato-u.ac.jp
authors.forename: Masao
authors.initials: M
authors.lastname: Hirotani
authors.forename: Kou
authors.initials: K
authors.lastname: Sai
authors.forename: Reiko
authors.initials: R
authors.lastname: Nagai
authors.forename: Seiko
authors.initials: S
authors.lastname: Hirotani
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takayanagi
authors.forename: Takafumi
authors.initials: T
authors.lastname: Yoshikawa
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D018360:Crystallography, X-Ray; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D025282:Mycelium / Q000737:chemistry*; D013256:Steroids / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D013256:Steroids