title: Biotinylated Stealth magnetoliposomes.
abstract: Dimyristoylphosphatidylethanolamine (DC(14:0)PE) and the dioleoyl analogue (DC(18:1cis)PE) were mixed with alpha-biotinylamido-omega-N-succinimidoxycarbonyl-poly(ethylene glycol) (NHS-PEG-biotin) and quantitatively converted to alpha-biotinylamido-omega-(dimyristoylphosphatidylethanolamino-carbonyl)polyethylene glycol (DC(14:0)PE-PEG-biotin) and the dioleoyl analogue DC(18:1cis)PE-PEG-biotin, respectively. As shown by thin-layer chromatography and 1H NMR spectroscopy, PEGylation of both phosphatidylethanolamine types went to completion if the reaction was performed in organic solvent in the presence of triethylamine. The resulting derivatives were successfully incorporated into both classical phospholipid vesicles and a phospholipid bilayer surrounding nanometer-sized magnetite cores. In the latter case, the so-called activated Stealth(1) magnetoliposomes were produced which very efficiently immobilized streptavidinylated alkaline phosphatase.
authors.affiliation: Rheinisch-Westfälische Technische Hochschule Aachen, Prof. Pirlet-Strasse 1, D-52074, Aachen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Hodenius
authors.forename: Marcel
authors.initials: M
authors.lastname: De Cuyper
authors.forename: Linda
authors.initials: L
authors.lastname: Desender
authors.forename: Detlef
authors.initials: D
authors.lastname: Müller-Schulte
authors.forename: Alois
authors.initials: A
authors.lastname: Steigel
authors.forename: Heiko
authors.initials: H
authors.lastname: Lueken
mesh_terms: D000469:Alkaline Phosphatase; D001710:Biotin; D016503:Drug Delivery Systems / Q000379:methods*; D052203:Ferrosoferric Oxide; D007501:Iron; D008081:Liposomes / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D010087:Oxides; D011092:Polyethylene Glycols; D019809:Streptavidin
chemical_list: D008081:Liposomes; D010087:Oxides; D011092:Polyethylene Glycols; D001710:Biotin; D019809:Streptavidin; D007501:Iron; D000469:Alkaline Phosphatase; D052203:Ferrosoferric Oxide