Development and characterization of polymorphic microsatellite markers in taro (Colocasia esculenta).
Microsatellite-containing sequences were isolated from enriched genomic libraries of taro (Colocasia esculenta (L.) Schott). The sequencing of 269 clones yielded 77 inserts containing repeat motifs. The majority of these (81.7%) were dinucleotide or trinucleotide repeats. The GT/CA repeat motif was the most common, accounting for 42% of all repeat types. From a total of 43 primer pairs designed, 41 produced markers within the expected size range. Sixteen (39%) were polymorphic when screened against a restricted set of taro genotypes from Southeast Asia and Oceania, with an average of 3.2 alleles detected on each locus. These markers represent a useful resource for taro germplasm management, genome mapping, and marker-assisted selection.
Mace
Emma S
ES
M.S. Swaminathan Applied Genomics Laboratory, International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Andhra Pradesh, India. e.mace@cgiar.org
Godwin
Ian D
ID
D000483:Alleles; D001210:Asia, Southeastern; D001483:Base Sequence; D003001:Cloning, Molecular; D031060:Colocasia / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018900:Dinucleotide Repeats; D018745:Genome, Plant; D015698:Genomic Library; D018895:Microsatellite Repeats; D010139:Pacific Islands; D011110:Polymorphism, Genetic; D018911:Trinucleotide Repeats
D017931:DNA Primers; D018744:DNA, Plant