Antigen-driven murine CD4+ T lymphocyte proliferation and interleukin-2 production are diminished by dietary (n-3) polyunsaturated fatty acids.
This study is the first to describe the impact of consuming a diet rich in (n-3) polyunsaturated fatty acids (PUFA) from fish oil on antigen-driven activation of naive CD4+ T lymphocytes. To accomplish this, we used lymphocytes isolated from T cell receptor (TCR) transgenic mice (i.e., DO11.10). A large portion of the T lymphocytes from these mice expresses a TCR specific for a peptide within the ovalbumin (OVA) molecule (OVA(323-339)). When this antigen is presented in the context of major histocompatibility complex I-A(d) with costimulation, these naive CD4+ T cells become activated, produce interleukin (IL)-2 and clonally expand. (n-3) PUFA enrichment was accomplished by feeding DO11.10 mice one of two nutritionally complete experimental diets that differed only in the source of fat: lard or menhaden fish oil [high in (n-3) PUFA]. After 2 wk of consuming the experimental diets, lymphocytes were isolated from the spleen of each mouse, then cultured in the presence of antigen (i.e., OVA(323-339)) or concanavalin A (Con A), a nonspecific, polyclonal T cell stimulus. IL-2 production and lymphocyte proliferation were determined after 48 and 72 h, respectively. Naive CD4+ T lymphocytes from fish oil-fed mice stimulated with antigen produced less IL-2 ( approximately 33%; P < 0.001) and proliferated to a lesser extent ( approximately 50%; P < 0.0001) than the same cells from lard-fed DO11.10 mice. When stimulated with Con A, (n-3) PUFA did not affect either proliferation or IL-2 production. In summary, we report for the first time that feeding mice a diet enriched with (n-3) PUFA reduces in vitro antigen-stimulated production of IL-2 and subsequent proliferation of naive CD4+ T lymphocytes.
Pompos
Lisa J
LJ
Department of Animal Sciences, Nutritional Sciences and the Comparative Medicine Program, University of Missouri, Columbia, MO 65211, USA.
Fritsche
Kevin L
KL
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D015525:Fatty Acids, Omega-3 / Q000378:metabolism / Q000494:pharmacology*; D005434:Flow Cytometry; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008623:Mercaptoethanol / Q000494:pharmacology; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D010047:Ovalbumin / Q000276:immunology; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000378:metabolism
D000941:Antigens; D004042:Dietary Fats, Unsaturated; D015525:Fatty Acids, Omega-3; D007376:Interleukin-2; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; D003208:Concanavalin A; D008623:Mercaptoethanol; D010047:Ovalbumin