Inhibitory effect of vitamin D3 on 3'methyl-4-dimethyl-amino-azobenzene-induced rat hepatocarcinogenesis: a study on antioxidant defense enzymes.
The anticarcinogenic effect of vitamin D3 (VD3) on 3,-methyl-4-dimethyl-amino-azobenzene (3,-Met-DAB)-induced hepatocarcinogenesis was investigated in male Sprague-Dawley rats. Anticancer efficacy of VD3 was estimated using different possible biomarkers, namely reduced glutathione (GSH) concentration, gamma-glutamyl transpeptidase (GGT) activity, glutathione S-transferase (GST) activity, glutathione reductase (GRd) activity, glutathione peroxidase (GPx) activity in hyperplastic nodules (HNs) and non-nodular surrounding parenchyma (NNSP) liver areas. VD3 was found to control the carcinogen-induced alterations in GSH level, GST, GGT, GRd and GPx activity both in HNs and NNSP liver areas during long-term exposure. A decrease in the number of HNs was also evident in the present investigation. VD3 was proved to be an effective antitumor drug during the initiation/promotion phases of hepatic carcinogenesis but the effect was found to be less prominent during initiation and promotion phases.
Karmakar
Ranajit
R
Department of Zoology, Taki Government College, Taki, North 24 Parganas, West Bengal, India. rk_wbes@lycos.com
Banik
Sarmistha
S
Chatterjee
Malay
M
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced* / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002762:Cholecalciferol / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D005723:gamma-Glutamyltransferase / Q000378:metabolism; D010128:p-Aminoazobenzene / Q000031:analogs & derivatives* / Q000494:pharmacology*
D016588:Anticarcinogenic Agents; D000975:Antioxidants; D002762:Cholecalciferol; D010128:p-Aminoazobenzene; C008468:3-methyl-4-methylaminoazobenzene; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005723:gamma-Glutamyltransferase; D005982:Glutathione Transferase