Application of real time PCR determination to assess interanimal variabilities in CYP1A induction in the European flounder (Platichthys flesus).
In both laboratory experiments and field investigations with fish a large interanimal variability in CYP1A expression has been observed which may be attributed to variations in environmental inducer exposure and/or inducer response. We are carrying out laboratory investigations to assess the contribution of a potential genetic component in inducer response of flounder (Platichthy sflesus) CYP1A to PCB exposure and in this paper we report development of a sensitive quantitative RT-PCR procedure (real time PCR) where accumulation of the intercalated dye SYBR Green I is followed during cycling. Preliminary experiments using this procedure with artificially reared Arochlor 1254-treated flounders showed large interanimal differences in response for a single family group indicating that variability does have a genetic basis.
Dixon
T J
TJ
Institute of Aquaculture, University of Stirling, Scotland, UK.
Taggart
J B
JB
George
S G
SG
D000818:Animals; D013956:Antithyroid Agents / Q000378:metabolism / Q000494:pharmacology; D020111:Chlorodiphenyl (54% Chlorine) / Q000378:metabolism / Q000494:pharmacology; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis* / Q000235:genetics*; D004781:Environmental Exposure; D005432:Flounder / Q000235:genetics* / Q000502:physiology; D005786:Gene Expression Regulation; D007700:Kinetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D013956:Antithyroid Agents; D020111:Chlorodiphenyl (54% Chlorine); D019363:Cytochrome P-450 CYP1A1