Protective effect of moutan cortex extract on acetaminophen-induced cytotoxicity in human Chang liver cells.
The aim of this study was to investigate the effect of Moutan Cortex on acetaminophen (AAP)-induced toxicity in human Chang liver cells. Cells were incubated with AAP (0-30 mM) to evaluate the drug's ability to reduce cytoviability. For the cells treated with 10, 20 and 30 mM AAP, LDH leakage was 39.8%, 49.0% and 57.6%, respectively. Administration of Moutan Cortex reduced cytotoxicity in a dose-dependent manner. Glutathione (GSH) concentration in human liver cells decreased significantly after exposure to 20 (p<0.05) and 30 mM (p<0.01) AAP, and increased (p<0.05) if incubated with AAP and Moutan Cortex. The ability of AAP to inhibit mitochondrial function and its counteraction by Moutan Cortex was also evaluated. Moutan Cortex showed dose-dependent increases in MTT metabolism and ATP levels in AAP-treated cells. The DNA content of AAP-treated cells increased with the treatment of Moutan Cortex. These observations demonstrate that Moutan Cortex may significantly attenuate AAP-induced toxicity. It can be considered a cytoprotective agent in this in vitro model of drug toxicity.
Shon
Yun-Hee
YH
Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University, Kyongju, Korea.
Nam
Kyung-Soo
KS
D000082:Acetaminophen / Q000633:toxicity*; D002478:Cells, Cultured; D019610:Cytoprotection / Q000187:drug effects* / Q000502:physiology; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D029596:Paeonia
D004365:Drugs, Chinese Herbal; C038881:moutan cortex; D000082:Acetaminophen