The detection of phytohemagglutinin-lymphokine activated killer cells' in vitro antitumor action with MTT colorimetry.
OBJECTIVE: To detect the in vitro antitumor action of phytohemagglutinin and lymphokine activated killer (PHA- LAK) cells. METHOD: MTT colorimetric assay was used to detect the in vitro cytotoxicity of PHA-LAK cells on K562 MGc80-3 143TK Hela and LoVo. RESULTS: The significant cytotoxicity of PHA-LAK cells on these five tumor cells from different organs could be found in vitro. The PHA-LAK cell activity on 143TK could reach 57.3% when the ratio of effective cell (EC) to target cell (TC) was 7.5:1. The antitumor effect did not increased or even decreased when the ratio of EC to TC was 15:1. CONCLUSIONS: (1)PHA-LAK cell has non-specific cytotoxicity against tumor cells and can overcome the problems of the quantity and activity of immunocyte of traditional adoptive cellular immunotherapy. (2)Under some conditions does MTT colorimetric assay be a susceptible, simple and convenient method for detecting the cytotoxicity of immunocytes.
Wu
Jun
J
Department of Oncology, General Hospital of Guangzhou Command, Guangzhou 510010, China.
Wang
Xiao-Huai
XH
Yang
Tai-Chen
TC
Xian
Jiang
J