title: Characterization of a novel human UDP-GalNAc transferase, pp-GalNAc-T10.
abstract: A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) gene family was cloned as a homolog of human pp-GalNAc-T7, and designated pp-GalNAc-T10. pp-GalNAc-T10 transcript was found in the small intestine, stomach, pancreas, ovary, thyroid gland and spleen. In a polypeptide GalNAc-transfer assay, recombinant pp-GalNAc-T10 transferred GalNAc onto a panel of mucin-derived peptide substrates. Furthermore, pp-GalNAc-T10 demonstrated strong transferase activity with glycopeptide substrates.
authors.affiliation: Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Central-2, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan.
authors.forename: Lamei
authors.initials: L
authors.lastname: Cheng
authors.forename: Kouichi
authors.initials: K
authors.lastname: Tachibana
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jian ming
authors.initials: Jm
authors.lastname: Guo
authors.forename: Kahori
authors.initials: K
authors.lastname: Kahori Tachibana
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kameyama
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.forename: Toru
authors.initials: T
authors.lastname: Hiruma
authors.forename: Hiroko
authors.initials: H
authors.lastname: Iwasaki
authors.forename: Akira
authors.initials: A
authors.lastname: Togayachi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kudo
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narimatsu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics* / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000096:biosynthesis; D013379:Substrate Specificity; D014018:Tissue Distribution; D000097763:Polypeptide N-acetylgalactosaminyltransferase
chemical_list: D017350:N-Acetylgalactosaminyltransferases; D010455:Peptides; D012333:RNA, Messenger; D000097763:Polypeptide N-acetylgalactosaminyltransferase