[Environmental Isolates of Sporothrix schenckii in China].
Restriction fragment length polymorphism (RFLP) in mitochondrial DNA (mtDNA) from 25 environmental isolates of Sporothrix schenckii from northeastern China was investigated. Based on the mtDNA-RELP patterns with Hae III, 6 isolates were confirmed to be S. schenckii, while the other 19 isolates were confirmed to be species distinct from S. schenckii. The mtDNA RFLP patterns of the 19 non-S. schenckii were identical to each other. The non-S. schenckii isolates could not be discriminated from S. schenckii by their macro- or micro-morphological features, and were not pathogenic in guinea pigs. Serological and delayed hypersensitivity cross-reactions were found between S. schenckii and the non-S. schenckii species, suggesting antigenic similarity. These results indicate that RFLP analysis of mtDNA is essential for the identification of environmental isolates of S. schenckii.
Ishizaki
Hiroshi
H
Department of Dermatology, Kanazawa Medical University, Uchinada, Ishikawa 920-0293, Japan.
Kawasaki
Masako
M
Mochizuki
Takashi
T
Jin
Xue-Zhu
XZ
Kagawa
Saburo
S
D000818:Animals; D002681:China; D004271:DNA, Fungal / Q000032:analysis; D004272:DNA, Mitochondrial / Q000032:analysis; D006168:Guinea Pigs; D017720:Molecular Epidemiology / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D013175:Sporothrix / Q000235:genetics / Q000302:isolation & purification*; D013174:Sporotrichosis / Q000382:microbiology
D004271:DNA, Fungal; D004272:DNA, Mitochondrial