Electrochemical DNA biosensor for analysis of wastewater samples.
The application of a disposable electrochemical DNA biosensor to wastewater samples is reported. The DNA biosensor is assembled by immobilising double-stranded calf thymus DNA on the surface of a disposable, carbon screen-printed electrode (SPE). The oxidation signal of the guanine base, obtained by a square wave voltammetric scan, is used as analytical signal. The presence of compounds with affinity for DNA is measured by their effect on the guanine oxidation. The comparison of the results with a toxicity test based on bioluminescent bacteria has confirmed the applicability of the method to real samples.
Lucarelli
F
F
Dipartimento di Chimica/Polo Scientifico, Università degli studi di Firenze, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy.
Kicela
A
A
Palchetti
I
I
Marrazza
G
G
Mascini
M
M
D000818:Animals; D000873:Anthracenes / Q000032:analysis; D001559:Benzhydryl Compounds; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006147:Guanine / Q000737:chemistry; D007220:Industrial Waste / Q000032:analysis*; D008163:Luminescent Measurements; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis; D014865:Waste Disposal, Fluid; D014873:Water Pollutants / Q000032:analysis*; D000098826:Bisphenol A Compounds
D000873:Anthracenes; D001559:Benzhydryl Compounds; D004247:DNA; D006147:Guanine; D007220:Industrial Waste; D010636:Phenols; D014873:Water Pollutants; C006593:2-anthramine; C427203:calf thymus DNA; C006780:bisphenol A; D000098826:Bisphenol A Compounds