title: Aging and developmental transitions in the B cell lineage.
abstract: One explanation for the deterioration of the humoral immune response in elderly individuals is that B lymphopoiesis declines with increasing age. Recent studies report a dramatic decline in pre-B cell numbers in old mice. Surprisingly, the number of mature B cells does not decline with age. To determine if new B cells are made in aged animals despite the drop in pre-B cells, we used 5'-bromo-2-deoxyuridine labeling to determine the production rate of B cells in the bone marrow and spleen of young and old mice. Because of the great variability in the number of early B lineage cells in old mice, we acquired data on >60 young and 50 old mice throughout these experiments. The transitional and mature B cell compartments in the spleen have slower labeling kinetics in old mice as compared to young. By the end of 4 weeks of labeling, an average of only 15% of the mature B cell compartment consists of newly made cells compared to 30% in young mice. However, in contrast to an earlier report, our results indicate that there is no statistical difference in the rate of production of new immature B cells in the marrow of young and old animals. In total, our results confirm previous work showing that mature B cells in old mice have a slower turnover, but more importantly suggest that the defect in mature B cell turnover is not due to a decline in B lymphopoiesis, but rather an inability of the newly made cells to replenish the peripheral compartments.
authors.affiliation: Departments of Microbiology and Immunology, Loyola University Medical Center, Maywood, IL 60153, USA.
authors.forename: Kara M
authors.initials: KM
authors.lastname: Johnson
authors.forename: Kevin
authors.initials: K
authors.lastname: Owen
authors.forename: Pamela L
authors.initials: PL
authors.lastname: Witte
mesh_terms: D000375:Aging / Q000276:immunology*; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D001853:Bone Marrow / Q000276:immunology / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology*; D016922:Cellular Senescence / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D001973:Bromodeoxyuridine