Bone marrow transplant engraftment analysis with loss of an informative allele.
Short tandem repeats (STRs) are highly polymorphic DNA sequences in the human genome. STR genotype analysis is used for human identity testing and to monitor bone marrow engraftment after allogeneic transplantation. Engraftment analysis requires one or more informative STR loci that distinguish recipient from donor. The following case illustrates that chromosome loss in tumor cells during the course of disease may cause corresponding loss of an STR locus. This circumstance is a potential source of error in the interpretation of engraftment analysis, especially if only one informative allele is used to monitor engraftment.
Schichman
Steven A
SA
Pathology and Laboratory Medicine Service, Central Arkansas Veterans Healthcare System, Little Rock 72205, USA. schichman@exchange.UAMS.edu
Lin
Pei
P
Gilbrech
Lori J
LJ
Gray
Pamela S
PS
Wilson
Carla S
CS
Sawyer
Jeffrey R
JR
D000483:Alleles; D016026:Bone Marrow Transplantation; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D004247:DNA / Q000032:analysis; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D006085:Graft Survival / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D019656:Loss of Heterozygosity / Q000235:genetics*; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D020080:Tandem Repeat Sequences / Q000235:genetics*; D014019:Tissue Donors; D014180:Transplantation
D005819:Genetic Markers; D004247:DNA