The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway.
The Arabidopsis thaliana (L.) Heynh. mutant delayed-dehiscence2-2 (dde2-2) was identified in an En1/Spm1 transposon-induced mutant population screened for plants showing defects in fertility. The dde2-2 mutant allele is defective in the anther dehiscence process and filament elongation and thus exhibits a male-sterile phenotype. The dde2-2 phenotype can be rescued by application of methyl jasmonate, indicating that the mutant is affected in jasmonic acid biosynthesis. The combination of genetic mapping and a candidate-gene approach identified a frameshift mutation in the ALLENE OXIDE SYNTHASE (AOS) gene, encoding one of the key enzymes of jasmonic acid biosynthesis. Expression analysis and genetic complementation of the dde2-2 phenotype by overexpression of the AOS coding sequence confirmed that the male-sterile phenotype is indeed caused by the mutation in the AOS gene.
von Malek
Bernadette
B
Institute of Plant Biology, University of Zurich, Zollikerstr. 107, 8008 Zurich, Switzerland.
van der Graaff
Eric
E
Schneitz
Kay
K
Keller
Beat
B
D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D005298:Fertility / Q000235:genetics; D005816:Genetic Complementation Test; D019746:Intramolecular Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D054883:Oxylipins; D017386:Sequence Homology, Amino Acid
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid; C072239:methyl jasmonate; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase