Mast cell regulation via paired immunoglobulin-like receptor PIR-B.
Activating (PIR-A) and inhibitory (PIR-B) isoforms of the paired immunoglobulin (Ig)-like receptor family have been evaluated for their modulating potential in mast cell responses to IgE antibody and mast/stem cell growth factor (SCF). Mast cells produce PIR-A and PIR-B, but PIR-B was found to be predominantly expressed on the cell surface, where it was constitutively tyrosine phosphorylated and associated with SHP-1 tyrosine phosphatase. Efficient coligation of PIR-B with FcepsilonRI inhibited IgE-induced mast cell activation and serotonin release. PIR-B and c-kit (or mast/SCF receptor) coligation also inhibited SCF-induced mast cell responses. The PIR-B inhibitory activity was unimpaired in SHP-1-deficient mast cells, perhaps because of non-SHP-1-binding tyrosine-based inhibitory motif in the cytoplasmic tail of PIR-B. This analysis suggests that PIR-B may serve to control mast cell activity.
Chen
Ching-Cheng
CC
Department of Microbiology, University of Alabama at Birmingham, 35294, USA.
Kong
Dong-Won
DW
Cooper
Max D
MD
Kubagawa
Hiromi
H
D000818:Animals; D015870:Gene Expression; D047908:Intracellular Signaling Peptides and Proteins; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D051381:Rats; D017455:Receptors, IgE / Q000378:metabolism; D011971:Receptors, Immunologic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D047908:Intracellular Signaling Peptides and Proteins; C105948:Pira1 protein, mouse; C105949:Pirb protein, mouse; C491380:Pirb protein, rat; D017455:Receptors, IgE; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins; D019009:Proto-Oncogene Proteins c-kit; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; D017027:Protein Tyrosine Phosphatases; C509248:Ptpn6 protein, mouse; C509250:Ptpn6 protein, rat