Registration of serial tissue section images.
OBJECTIVE: This study aims to tackle the problem of image registration during computer-assisted three-dimensional (3D) reconstruction of serial tissue sections. METHODS: We proposed segmentation-counting algorithm for computerized image registration on the basis of joint histogram. This approach utilizes thresholding of the 2 images to be registered, and the criterion function is defined as the counting in a specific region of the joint histogram. The registration parameters can be obtained by optimizing the criterion function. RESULTS: In the trial application of this approach in image registration for the serial tissue sections of mouse wse embryos, a more efficient result was achieved. CONCLUSION: The method can rapidly accomplish the image registration task for serial tissue sections with simpler calculation processes.
Liu
Zhe-Xing
ZX
Key Lab for Medical Images, First Military Medical University, Guangzhou 510515, China.
Dong
Wu
W
Li
Shu-Xiang
SX
Jiang
Gui-Ping
GP
Zhang
Yu
Y
Xie
Xiao-Mian
XM