Analysis of the Escherichia coli Tol-Pal and TonB systems by periplasmic production of Tol, TonB, colicin, or phage capsid soluble domains.
The aim of this review is to describe an in vivo assay of the interactions taking place in the Tol-Pal or TonB-ExbB-ExbD envelope complexes in the periplasm of Escherichia coli and between them and colicins or g3p protein of filamentous bacteriophages. Domains of colicins or periplasmic soluble domains of Tol or TonB proteins can be artificially addressed to the periplasm of bacteria by fusing them to a signal sequence from an exported protein. These domains interact specifically in the periplasm with the Tol or TonB complexes and disturb their function, which can be directly detected by the appearance of specific tol or tonB phenotypes. This technique can be used to detect new interactions, to characterize them biochemically and to map them or to induce tol or tonB phenotypes to study the functions of these two complexes.
Bouveret
Emmanuelle
E
LISM, CNRS, 31, chemin Joseph-Aiguier, 13402, Marseille, cedex 20, France. bouveret@ibsm.cnrs-mrs.fr
Journet
Laure
L
Walburger
Anne
A
Cascales
Eric
E
Bénédetti
Hélène
H
Lloubès
Roland
R
D001426:Bacterial Proteins / Q000378:metabolism*; D036022:Capsid Proteins / Q000378:metabolism*; D003087:Colicins / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D019897:Periplasm / Q000378:metabolism; D014760:Viral Fusion Proteins / Q000378:metabolism
D001426:Bacterial Proteins; D036022:Capsid Proteins; D003087:Colicins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C052874:ExbB protein, E coli; D008565:Membrane Proteins; D014760:Viral Fusion Proteins; C062093:tolA protein, E coli; C057616:tonB protein, Bacteria; C489181:tonB protein, E coli; C060790:exbD protein, E coli