Cloning of full length cDNA sequence of the mouse ameloblastin.
OBJECTIVE
Screening for special genes of matrix proteins of dentin and enamel of mouse dental germ.


METHODS
A cDNA library of dental germ of mouse was screened by differential display. The interesting clones were sequenced.


RESULTS
Six positive clones were isolated from the cDNA library. The sequence of one of the six positive clones was homologous with the ameloblastin sequence of rat. There are 497 homologous base pairs between the 526 base pairs sequenced by pTriplEX 3' primer of this clone and the 32-580 sequence of the rat ameloblastin gene; and there are 533 homologous base pairs between the 567 base pairs sequenced by pTriplEX 5' primer of this clone and the 1285-1854 sequence of the rat ameloblastin gene.


CONCLUSIONS
The full length cDNA sequence of the mouse ameloblastin was cloned.
Gu
Shuping
S
Department of Oral Medicine, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Shi
Junnan
J
Liu
Han
H
Hao
Jianjun
J
Wang
Ping
P
Fei
Jian
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015723:Gene Library; D051379:Mice; D008969:Molecular Sequence Data
D018076:DNA, Complementary