Interference of thiomersal in biologicals during protein estimation.
In the present report thiomersal was detected as interfering substance in hepatitis B vaccines during the total protein estimation by Lowry's protein assay. The thiomersal at different concentrations of 0.005%, 0.0075%, 0.01%, 0.02%, 0.05% and 0.1% was found to reduce the Folin Ciocalteu's phenol reagent and produce colour development between 0.024 O.D to 1.023 O.D. values. Further, the thiomersal was shown to interfere between 34.55% to 52.73% with Folin Ciocalteu's phenol reagent, when 10 batches of different hepatitis B vaccines were subjected to estimation of total protein content by Lowry's protein assay.
Dogar
Vikas
V
Central Drugs Laboratory, Central Research Institute, Kasauli (H.P.), 173204, India.
Sokhey
Jaspal
J
Kumar
Sanjeev
S
Sood
Dinesh Kumar
DK
Tahlan
Ajay Kumar
AK
D001688:Biological Products / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D017325:Hepatitis B Vaccines / Q000032:analysis; D007202:Indicators and Reagents; D008982:Molybdenum; D011506:Proteins / Q000032:analysis*; D013849:Thimerosal / Q000032:analysis*; D017973:Tungsten Compounds; D014764:Viral Proteins / Q000032:analysis
D001688:Biological Products; C029556:Folin's phenol reagent; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D007202:Indicators and Reagents; D011506:Proteins; D017973:Tungsten Compounds; D014764:Viral Proteins; D013849:Thimerosal; D008982:Molybdenum