title: Measurement of erythrocyte lipids, lipid peroxidation, antioxidants and osmotic fragility in cervical cancer patients.
abstract: BACKGROUND
  Our aim was to examine the structural integrity of red blood cells in cervical cancer patients by measuring the concentrations of thiobarbituric acid reactive substances (TBARS), antioxidant status, cholesterol/phospholipid (C/P) molar ratio, enzyme activity and osmotic fragility of erythrocytes.
  
  
  METHODS
  This study has been conducted on 32 adult female cervical cancer patients and an equal number of age- and sex-matched normal subjects. Erythrocyte concentrations of lipids, TBARS, vitamin E, reduced glutathione and enzymic activities of catalase and Na(+)K(+)-ATPase were measured as well as plasma concentrations of sodium and potassium. The present study also examined the changes in erythrocyte osmotic fragility in cervical cancer patients and normal subjects. The red cell fluidity and permeability were determined by estimating the C/P ratio and Na(+)K(+)-ATPase activity, respectively.
  
  
  RESULTS
  The release of thiobarbituric acid reactive substances was significantly higher in cervical cancer patients as compared to normal subjects. The increased lipid peroxidation with concomitant decrease in antioxidants was notable in cervical cancer patients. Red blood cells of cervical cancer patients were more fragile than those from normal subjects. Increase in red cell membrane C/P ratio and Na(+)K(+)-ATPase activity was noticed in cervical cancer patients as compared to normal subjects.
  
  
  CONCLUSIONS
  Increased lipid peroxidation, insufficient antioxidant potential and changes in C/P molar ratio as well as activity of Na(+)K(+)-ATPase cause structural and functional abnormalities in the erythrocytes of cervical cancer patients.
authors.affiliation: Department of Biochemistry, Annamalai University, Annamalai Nagar, Tamilnadu 608 002, India.
authors.forename: K
authors.initials: K
authors.lastname: Kolanjiappan
authors.forename: S
authors.initials: S
authors.lastname: Manoharan
authors.forename: M
authors.initials: M
authors.lastname: Kayalvizhi
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood; D004910:Erythrocyte Membrane / Q000201:enzymology / Q000378:metabolism*; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D008055:Lipids / Q000097:blood*; D008563:Membrane Lipids / Q000097:blood; D008875:Middle Aged; D009996:Osmotic Fragility; D010743:Phospholipids / Q000097:blood; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000097:blood* / Q000201:enzymology
chemical_list: D000975:Antioxidants; D008055:Lipids; D008563:Membrane Lipids; D010743:Phospholipids; D017392:Thiobarbituric Acid Reactive Substances; D002784:Cholesterol; D000254:Sodium-Potassium-Exchanging ATPase