[Expression and significance of bcl-2 and bcl-xL in rat neuron after acute brain trauma].
OBJECTIVE
To observe the expression of bcl-2 and bcl-xL in rat brain neuron after acute brain trauma.


METHODS
The rat model of mild and sever brain trauma were made by diffused brain injury. The expression of bcl-2 and bcl-xL in rat brain neuron was examined by immunohistochemical staining. Rat neuron apoptosis was detected by TUNEL method.


RESULTS
There were few bcl-2 (cortex: 4.40 +/- 1.67, hippocampal: 3.20 +/- 1.30) and lots of bcl-xL (cortex: 45.60 +/- 4.34, hippocampal: 50.20 +/- 3.50) expression in normal controls. After impact, the expression of bcl-2 in rat brain neuron increased, most distinctly on day 1(cortex: 30.0 +/- 4.3, hippocampal: 46.6 +/- 3.2), in mild group. A negative correlation was seen between bcl-2 expression and neuronal apoptosis (-1 < r < -0.847, P< 0.01, n = 10). No change was seen in bcl-xL expression.


CONCLUSIONS
bcl-2 expression increased after acute brain trauma but bcl-xL did not change much. Both bcl-2 and bcl-xL are concerned with anti-apoptosis in neuron after acute brain trauma.
Yang
Xinyu
X
Department of Neurosurgery, General Hospital, Tianjin Medical University, Tianjin 300052, China.
Yang
Shuyuan
S
Zhang
Jianning
J
Xue
Liang
L
D000208:Acute Disease; D000818:Animals; D017209:Apoptosis; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000737:chemistry* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D051020:bcl-X Protein
C494813:Bcl2l1 protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein