Differential Akt phosphorylation at Ser473 and Thr308 in cultured neurons after exposure to glutamate in rats.
Akt kinase is involved in growth factor-mediated neuronal protection. In the present study, we found in cultured neurons exposed to glutamate, that phosphorylation at Ser473 was transiently induced, but the level of phosphorylation at Thr308 and Akt activity were unchanged. Inhibition of phosphoinositide 3-kinase with LY294002 decreased phosphorylation and Akt activity, however, pretreatment with LY294002 did not affect glutamate toxicity. Our findings suggested that the endogenous Akt pathway does not play a crucial role in cell survival after exposure to glutamate.
Kitagawa
Kazuo
K
Division of Strokology, Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine (A8), Yamadaoka 2-2, Suita-city, Osaka 565-0871, Japan. kitagawa@medone.med.osaka-u.ac.jp
Takasawa
Kenichiro
K
Kuwabara
Keisuke
K
Sasaki
Tsutomu
T
Tanaka
Shigeru
S
Mabuchi
Takuma
T
Sugiura
Shiro
S
Omura-Matsuoka
Emi
E
Matsumoto
Masayasu
M
Hori
Masatsugu
M
D000818:Animals; D015153:Blotting, Western; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D002867:Chromones / Q000494:pharmacology; D017362:Cyclic AMP Response Element-Binding Protein / Q000187:drug effects / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology* / Q000633:toxicity; D038362:Glycogen Synthase Kinase 3 / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D009025:Morpholines / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins / Q000187:drug effects* / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt; D051381:Rats; D017208:Rats, Wistar; D012694:Serine / Q000378:metabolism; D013912:Threonine / Q000378:metabolism; D013997:Time Factors
D002867:Chromones; D017362:Cyclic AMP Response Element-Binding Protein; D004791:Enzyme Inhibitors; D009025:Morpholines; D011518:Proto-Oncogene Proteins; D013912:Threonine; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D018698:Glutamic Acid; D012694:Serine; C494920:Akt1 protein, rat; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt; D038362:Glycogen Synthase Kinase 3; C448021:glycogen synthase kinase 3 alpha