title: Evaluation of methods to increase the sensitivity and timeliness of detection of Streptococcus agalactiae in pregnant women.
abstract: Direct culture of rectovaginal specimens on Granada agar was compared to culture on sheep blood agar plate (SBAP) and AccuProbe detection of group B streptococcus from overnight LIM broth enhancement cultures (LIM-SBAP). Both broth-enhanced methods demonstrated excellent sensitivity (97.5% for LIM-SBAP and 93.5% for AccuProbe), while Granada agar demonstrated a sensitivity of only 40.3%.
authors.affiliation: University of Kentucky Hospital. Department of Pathology and Laboratory Medicine, University of Kentucky, Lexington, Kentucky 40536-0293, USA.
authors.forename: Sue B
authors.initials: SB
authors.lastname: Overman
authors.forename: Douglas D
authors.initials: DD
authors.lastname: Eley
authors.forename: Barry E
authors.initials: BE
authors.lastname: Jacobs
authors.forename: Julie A
authors.initials: JA
authors.lastname: Ribes
mesh_terms: D000362:Agar; D001431:Bacteriological Techniques; D003470:Culture Media; D015342:DNA Probes / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D006801:Humans; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology*; D012335:RNA, Ribosomal / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D012007:Rectum / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000382:microbiology*; D013292:Streptococcus agalactiae / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D013997:Time Factors; D014621:Vagina / Q000382:microbiology*
chemical_list: D003470:Culture Media; D015342:DNA Probes; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D011933:Reagent Kits, Diagnostic; D000362:Agar