title: Effect of E1B mutant adenovirus on p53-deficient leukemic cells.
abstract: OBJECTIVE: To study the effect of E1B mutant adenovirus (dl1520) on 3 p53-deficient leukemic cell lines K562, Jurkat and HL-60. METHODS: The replication efficiency of dl1520 in the 3 leukemic cell lines was assessed by plaque assay, and the number of cells killed by the adenovirus determined by using trypan-blue in a course of 10 d following the infection. RESULTS: The replication efficiency of dl1520 in the 3 leukemic cell lines was significantly lower than that in the positive control, and no significant cytocidal effect against the 3 cell lines was observed. CONCLUSION: dl1520 can not inhibit the malignant blood cells as K562, Jurkat and HL-60 cell lines.
authors.affiliation: Institute of Pharmaceutic Science, First Military Medical University, Guangzhou 510515, China.
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Rao
authors.forename: Zheng-Guang
authors.initials: ZG
authors.lastname: Zhu
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Wu