title: Cloning of a gene encoding acetate kinase from Methanosarcina mazei 2-P isolated from a Japanese paddy field soil.
abstract: The ack gene encoding acetate kinase from the mesophilic Methanosarcina mazei 2-P, isolated from a paddy field soil in Japan, was cloned, sequenced, and functionally expressed in Escherichia coli. The terminal region of the putative pta gene, probably encoding phosphotransacetylase, was found upstream of the ack gene. The deduced amino acid sequence of the acetate kinase is 86.5% identical to that of the Methanosarcina thermophila acetate kinase. The activity of the His(6)-tagged acetate kinase purified from E. coli JM109 was optimal at 35 degrees C.
authors.affiliation: Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Japan. symbio@cc.hirosaki-u.ac.jp
authors.forename: Akio
authors.initials: A
authors.lastname: Tonouchi
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Nishizaki
authors.forename: Hiromi
authors.initials: H
authors.lastname: Tohyama
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Takeda
mesh_terms: D000084:Acetate Kinase / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007564:Japan; D017020:Methanosarcina / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D000084:Acetate Kinase