title: Cloning and phylogenetic analysis of the ribosomal internal transcribed spacer-1 (ITS1) of Cryptosporidium wrairi and its relationship to C. parvum genotypes.
abstract: We have cloned and sequenced the ribosomal internal transcribed spacer-1 (ITS1) of Cryptosporidium wrairi. Phylogenetic analysis of this region provided further support to the validity of C. wrairi as a distinct species and also to the concept that many of the genotypes recently identified within C. parvum are in fact separate species. Analysis placed the "cattle" and "mouse" genotypes of C. parvum as each other's closest relatives and C. wrairi as a sister group to these two genotypes, followed by the "human" genotype.
authors.affiliation: Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA, Australia.
authors.forename: U M
authors.initials: UM
authors.lastname: Morgan-Ryan
authors.forename: P
authors.initials: P
authors.lastname: Monis
authors.forename: A
authors.initials: A
authors.lastname: Possenti
authors.forename: A
authors.initials: A
authors.lastname: Crisanti
authors.forename: F
authors.initials: F
authors.lastname: Spano
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D003457:Cryptosporidiosis / Q000469:parasitology / Q000662:veterinary; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics*; D016785:Cryptosporidium parvum / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D004196:Disease Outbreaks; D005838:Genotype; D006168:Guinea Pigs; D006801:Humans; D016013:Likelihood Functions; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012376:Rodent Diseases / Q000469:parasitology; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014158:Transcription, Genetic
chemical_list: D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer