Incorporation of [1-14C]-linoleic acid by LLC-WRC256 tumour cells.
The incorporation of [(14)C]-linoleic acid (LA) into total lipid fractions was higher in LLC-WRC256 cells from the log phase of growth as compared to those of the plateau phase. LA was mainly incorporated into the phospholipid (PL) fraction of cells during the log phase, whereas in the plateau phase it was mostly taken into cholesterol ester. The proportion of radioactivity was higher in phosphatidylserine of cells from the log phase, whereas in the plateau phase it was higher in phosphatidylcholine. This feature of LA incorporation may be an important factor in determining the proliferative capacity of tumour cells.
da Costa
Marilena
M
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Av, Prof. Lineu Prestes 1524, 05508-900 Butantã, São Paulo, SP, Brazil.
Vecchia
Marilene G
MG
Peres
Carmem M
CM
Curi
Rui
R
D000818:Animals; D002250:Carbon Radioisotopes / Q000494:pharmacology; D002455:Cell Division; D002788:Cholesterol Esters / Q000378:metabolism; D002845:Chromatography; D019787:Linoleic Acid / Q000494:pharmacology*; D010718:Phosphatidylserines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D013997:Time Factors; D014407:Tumor Cells, Cultured
D002250:Carbon Radioisotopes; D002788:Cholesterol Esters; D010718:Phosphatidylserines; D010743:Phospholipids; D019787:Linoleic Acid