title: The Caenorhabditis elegans presenilin sel-12 is required for mesodermal patterning and muscle function.
abstract: Mutations in presenilin genes impair Notch signalling and, in humans, have been implicated in the development of familial Alzheimer's disease. We show here that a reduction of the activity of the Caenorhabditis elegans presenilin sel-12 results in a late defect during sex muscle development. The morphological abnormalities and functional deficits in the sex muscles contribute to the egg-laying defects seen in sel-12 hermaphrodites and to the severely reduced mating efficiency of sel-12 males. Both defects can be rescued by expressing sel-12 from the hlh-8 promoter that is active during the development of the sex muscle-specific M lineage, but not by expressing sel-12 from late muscle-specific promoters. Both weak and strong sel-12 mutations cause defects in the sex muscles that resemble the defects we found in lin-12 hypomorphic alleles, suggesting a previously uncharacterised LIN-12 signalling event late in postembryonic mesoderm development. Together with a previous study indicating a role of lin-12 and sel-12 during the specification of the pi cell lineage required for proper vulva-uterine connection, our data suggest that the failure of sel-12 animals to lay eggs properly is caused by defects in at least two independent signalling events in different tissues during development.
authors.affiliation: ABI, Department of Biochemistry, Laboratory of Molecular Neurogenetics, Ludwig-Maximilians-Universitaet Munich, Schillerstrasse 44, Munich D-80336, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Eimer
authors.forename: Roland
authors.initials: R
authors.lastname: Donhauser
authors.forename: Ralf
authors.initials: R
authors.lastname: Baumeister
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000235:genetics*; D017173:Caenorhabditis elegans / Q000196:embryology* / Q000235:genetics; D029742:Caenorhabditis elegans Proteins; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015801:Helminth Proteins / Q000235:genetics*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008648:Mesoderm / Q000502:physiology; D009132:Muscles / Q000196:embryology / Q000502:physiology; D009154:Mutation
chemical_list: D029742:Caenorhabditis elegans Proteins; D015801:Helminth Proteins; D008565:Membrane Proteins; C095907:SEL-12 protein, C elegans