[Histochemical assessment of immunoreactivity of thyroid peroxidase and its correlation with biochemical activity].
BACKGROUND
Peroxidase content has been recently evaluated in normal thyroid and in different thyroid disorders by biochemical, histochemical, ultrastructural and immunocytochemical methods Nevertheless immunocytochemical detection of thyroid peroxidase in thyroid samples conventionally processed for histology hes never been done using a commercially available antibody, neither its correlation with the biochemical activity on adjacent samples.


METHODS
In this study we have analyzed normal thyroid tissue (3 patients), follicular adenoma (2 patients) and multinodular goiter (2 patients) conventionally processed for histology and stiined by immunocytochemistry (Avidin Biotin System) using a polyclonal (rabbit) antibody for horseradish peroxidase (Serotec). Biochemical assay was performed on adjacent samples according to Hosoya method.


RESULTS
Normal thyroid showed peroxidase immunoresectivity in the majority of follicular celis; neoplastic cells of adenomas were variably stained. Biochemical assay showed positive correlation with ICC ranging from 20.4 mg/mg/prot a in multinodular goiter to 42.12 in normal thyroid, up to 122 of follicular adenoma.


CONCLUSIONS
Peroxidase content in the thyroid gland may be of clinical interest in several thyroid diseases, and in this study we have demonstrated that thyroid peroxidase can be detected by ICC in routinely processed thyroid samples using a commercially available antibody.
Carlei
F
F
P.O. Anagni Asl FR, Divisione di Chirurgia Geriatrica, Università degli Studi di L'Aquila.
Perata
E
E
Schietroma
M
M
Ventura
T
T
Natuzzi
G
G
Rossi
M
M
Capperucci
G
G
Lezoche
E
E
D006801:Humans; D007150:Immunohistochemistry; D007453:Iodide Peroxidase / Q000276:immunology*; D013961:Thyroid Gland / Q000276:immunology* / Q000473:pathology*
D007453:Iodide Peroxidase