title: [Expression of histone-based fusion protein HNHG in E.coli].
abstract: Histone H1 contributes to condense nucleosome into super-structure during the transformation of chromatin into chromosome. It is shown in this rep or t that the fusion protein HNHG with the core of C-terminus of histone H1(0) expressed in BL21 (DE3) could also condense the plasmid DNA, just as histones did in nucleus. Under electron microscope, plasmid DNA condensed and supercoiled after t he addition of HNHG, in contrast to plasmid DNA control. This specific ability of the fusion protein HNHG of binding and condensing plasmids could be utilized to construct novel exogenous gene delivery systems. HNHG would be a promising candidate for gene delivery.
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032, China. nlorg@public.sta.net.cn
authors.forename: Fei-Han
authors.initials: FH
authors.lastname: Dai
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Gong
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Yang
authors.forename: Pei-Kun
authors.initials: PK
authors.lastname: Tian
authors.forename: Jian-Ren
authors.initials: JR
authors.lastname: Gu
mesh_terms: D004278:DNA, Superhelical / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D024202:Electrophoretic Mobility Shift Assay; D015870:Gene Expression; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008854:Microscopy, Electron; D010957:Plasmids / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D004278:DNA, Superhelical; D006657:Histones; D011993:Recombinant Fusion Proteins