Apoptosis-specific protein (ASP 45 kDa) is distinct from human Apg5, the homologue of the yeast autophagic gene apg5.
We have examined whether the apoptosis-specific protein p45ASP and human Apg5 are identical proteins. Like p45ASP, myc-hApg5 cross-reacted with a c-Jun antibody and approximately 50% of myc-hApg5 was bound to a Triton X-100-insoluble fraction in HeLa cells. However, soluble myc-hApg5 was degraded during apoptosis induced by staurosporine or TNFalpha/cycloheximide whilst expression of soluble p45ASP was stabilised. Furthermore, myc-hApg5 degradation was blocked by the caspase inhibitor Boc-Asp(OMe)FMK whilst p45ASP expression was eliminated. Moreover, myc-hApg5 ( approximately 32 kDa) never assumed the size of p45ASP (45 kDa). It is therefore likely that p45ASP and human Apg5 are distinct proteins although they do share some common characteristics.
Yung
Hong Wa
HW
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, UK.
Xue
Luzheng
L
Tolkovsky
Aviva M
AM
D017209:Apoptosis; D000071187:Autophagy-Related Protein 5; D053148:Caspase 3; D020169:Caspases / Q000494:pharmacology; D003429:Cross Reactions; D003598:Cytoskeletal Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D005800:Genes, Fungal; D006367:HeLa Cells; D006801:Humans; D011506:Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000032:analysis; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D044767:Ubiquitin-Protein Ligases
C102268:ATG5 protein, S cerevisiae; D000071187:Autophagy-Related Protein 5; D003598:Cytoskeletal Proteins; D011506:Proteins; D016755:Proto-Oncogene Proteins c-jun; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D044767:Ubiquitin-Protein Ligases; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases