title: Effect of recombinant human interleukin-10 on the in vitro proliferation of rat vascular smooth muscle cells.
abstract: OBJECTIVE: To observe the effects of recombinant human interleukin-10 (rhIL-10) on the proliferation of rat vascular smooth muscle cells (VSMCs) cultured in vitro. METHOD: Aortic VSMCs cultured in vitro were treated with varied doses of rhIL-10 alone or in combination with tumor necrosis factor-alpha (TGF-alpha) or platelet-derived growth factor-BB(PDGF- BB) respectively. The VSMC proliferation was quantified by colormetric assay. RESULTS: Compared with control, both TNF-alpha and PDGF-BB stimulated conspicuous proliferation of VSMCs, but the effect of which was not observed by rhIL-10 treatment alone. In the presence of rhIL-10, the proliferation effects of both TGF-alpha and PDGF-BB on VSMCs were significantly inhibited (P<0.05). CONCLUSION: rhIL-10 can inhibit VSMC proliferation induced by TNF-alpha and PDGF-BB, thus may provide a new therapeutic approach for regulating vascular wall remodeling after vascular injury.
authors.affiliation: Department of Cardiology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ouyang
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Lai
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Peng
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Wu
authors.forename: An-Long
authors.initials: AL
authors.lastname: Xu