title: [Bluetongue: isolation and characterization of the virus and identification of vectors in northeastern Argentina].
abstract: To establish if BTV was circulating in Argentina, 94 bovines from the Santo Tomé and Ituzaingó Departments of Corrientes Province were sampled every 30-60 days during 14 months. Red blood cells from those animals that showed seroconvertion with a c-ELISA were processed for virus isolation by inoculation in embryonated chicken eggs and cell cultures. Cells with CPE were positive by direct and indirect immunofluorescence with BTV specific reagents. These samples examined by electron microscopy showed virus particles with BTV morphological characteristics. Blood samples and tissue culture supernantants were positive by RT-PCR technique with primers corresponding to the segment 3 of the BTV genome. Haematophagous insects were captured in one farm using light traps and Culicoides insignis Lutz was the predominant species detected. This is the first isolation of BTV in Argentina from northeastern bovines without any disease symptom.
authors.affiliation: Institutos de Virología y Patobiología, CICVyA, INTA-Castelar, CC25, 1712 Castelar, Argentina.
authors.forename: C
authors.initials: C
authors.lastname: Gorch
authors.forename: A
authors.initials: A
authors.lastname: Vagnozzi
authors.forename: S
authors.initials: S
authors.lastname: Duffy
authors.forename: J
authors.initials: J
authors.lastname: Miquet
authors.forename: J
authors.initials: J
authors.lastname: Pacheco
authors.forename: A
authors.initials: A
authors.lastname: Bolondi
authors.forename: G
authors.initials: G
authors.lastname: Draghi
authors.forename: B
authors.initials: B
authors.lastname: Cetra
authors.forename: C
authors.initials: C
authors.lastname: Soni
authors.forename: M
authors.initials: M
authors.lastname: Ronderos
authors.forename: S
authors.initials: S
authors.lastname: Russo
authors.forename: V
authors.initials: V
authors.lastname: Ramírez
authors.forename: I
authors.initials: I
authors.lastname: Lager
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001118:Argentina / Q000453:epidemiology; D001819:Bluetongue / Q000453:epidemiology / Q000635:transmission / Q000821:virology*; D001820:Bluetongue virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000635:transmission / Q000821:virology*; D002478:Cells, Cultured / Q000821:virology; D002519:Ceratopogonidae / Q000821:virology*; D002645:Chickens; D004531:Eggs; D004797:Enzyme-Linked Immunosorbent Assay; D016679:Genome, Viral; D007303:Insect Vectors / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D012621:Seasons; D014776:Virus Cultivation
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral