title: Mechanism of copper resistance in a copper mine isolate Pseudomonas putida strain S4.
abstract: The mechanism of copper resistance in a multiple-metal-resistant natural isolate Pseudomonas putida strain S4 is based on inducible efflux. Active extrusion of copper ions occurs from the cytoplasm during the exponential phase of growth. Involvement of ATPase in the efflux of copper ions has been demonstrated by employing specific inhibitors. The effluxed copper is not thrown out of the cell, but remains in a bound form (to a protein) in the periplasm. Thus, a balance between the intracellular level, to fulfill the metabolic requirements, and the periplasmic sequestration, to evade toxicity, is maintained by this isolate.
authors.affiliation: Department of Genetics, University of Delhi South Campus, New Delhi-110021, India.
authors.forename: Deepa
authors.initials: D
authors.lastname: Saxena
authors.forename: Nalini
authors.initials: N
authors.lastname: Joshi
authors.forename: Sheela
authors.initials: S
authors.lastname: Srivastava
mesh_terms: D003300:Copper / Q000378:metabolism* / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D008906:Mining; D019897:Periplasm / Q000378:metabolism; D016958:Pseudomonas putida / Q000187:drug effects* / Q000254:growth & development
chemical_list: D003300:Copper