title: Selective eosinophil adhesion to fibroblast via IFN-gamma-induced galectin-9.
abstract: Among galectin family members, galectin-9 was first described as a potent eosinophil chemoattractant derived from Ag-stimulated T cells. In the present study a role of galectin-9 in the interaction between eosinophils and fibroblasts was investigated using a human lung fibroblast cell line, HFL-1. RT-PCR, real-time PCR, and Western blot analyses revealed that both galectin-9 mRNA and protein in HFL-1 cells were up-regulated by IFN-gamma stimulation. On the one hand, IL-4, known as a Th2 cytokine, did not affect the galectin-9 expression in HFL-1 cells. We further confirmed that IFN-gamma up-regulated the expression of galectin-9 in primary human dermal fibroblasts. Flow cytometric analysis revealed that IFN-gamma up-regulated surface galectin-9 expression on HFL-1 cells. Stimulation of HFL-1 cells with IFN-gamma up-regulated adhesion of eosinophils, but not neutrophils, to HFL-1 cells. This adherence of eosinophils to HFL-1 cells was inhibited by both lactose and anti-galectin-9 Ab. These findings demonstrate that IFN-gamma-induced galectin-9 expression in fibroblasts mediates eosinophil adhesion to the cells, suggesting a crucial role of galectin-9 in IFN-gamma-stimulated fibroblasts as a physiological modulator at the inflammatory sites.
authors.affiliation: Department of Radiology, Kagawa Medical University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Asakura
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Kashio
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakamura
authors.forename: Masako
authors.initials: M
authors.lastname: Seki
authors.forename: Shuyan
authors.initials: S
authors.lastname: Dai
authors.forename: Yukako
authors.initials: Y
authors.lastname: Shirato
authors.forename: Mohammad J
authors.initials: MJ
authors.lastname: Abedin
authors.forename: Naoko
authors.initials: N
authors.lastname: Yoshida
authors.forename: Nozomu
authors.initials: N
authors.lastname: Nishi
authors.forename: Tadaatsu
authors.initials: T
authors.lastname: Imaizumi
authors.forename: Naoki
authors.initials: N
authors.lastname: Saita
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Toyama
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Takashima
authors.forename: Takanori
authors.initials: T
authors.lastname: Nakamura
authors.forename: Motoomi
authors.initials: M
authors.lastname: Ohkawa
authors.forename: Mitsuomi
authors.initials: M
authors.lastname: Hirashima
mesh_terms: D000328:Adult; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D002474:Cell-Free System / Q000737:chemistry / Q000502:physiology; D002631:Chemotactic Factors, Eosinophil / Q000096:biosynthesis / Q000302:isolation & purification / Q000502:physiology; D004804:Eosinophils / Q000502:physiology*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology*; D037161:Galectins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008168:Lung / Q000378:metabolism / Q000473:pathology; D017563:Lung Diseases, Interstitial / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology; D011657:Pulmonary Eosinophilia / Q000378:metabolism / Q000473:pathology
chemical_list: D002631:Chemotactic Factors, Eosinophil; D037161:Galectins; C105032:LGALS9 protein, human; D007371:Interferon-gamma