Influence of light-dark cycle alteration on free radical level in rat cns.
The experiment analysed the evolution of several redox parameters caused by light-dark cycle alteration in different rat brain segments: cortex, brain stem, diencephalon. Continuous light (L:L) enhanced SOD, CAT, GPX and GSH levels to different extents in the various central nervous system regions investigated, depending on the entrainment period: 24 h, 48 h, 72 h, 7 days. Continuous darkness (D:D -24 h, 48 h, 72 h, 7 days) generally diminished SOD, CAT and GSH values, but increased GPX at 48 h and CAT at 72 h. MDA levels were higher in D:D, too. After 7 days L:L or D:D redox indices tended to reach the normal range again. Redox system spatio-temporal versatility and complementarity suggested the existence of a possible biochemical radical cell clock gear.
Baran
D
D
Gr. T. Popa University of Medicine and Pharmacy, Iaşi, Romania.
Paduraru
I
I
Saramet
A
A
Petrescu
E
E
Haulica
I
I
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001933:Brain Stem / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D002940:Circadian Rhythm / Q000502:physiology*; D004027:Diencephalon / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D010100:Oxygen / Q000378:metabolism; D017440:Photoperiod; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D005609:Free Radicals; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D010100:Oxygen