Immunomodulating properties of Argentine plants with ethnomedicinal use.
Five Argentine medicinal plants selected according to folk traditional or ethnomedical use, references and primary pharmacological screening; were chosen to elucidate their immunomodulating properties. Dichloromethane, methanolic and aqueous extracts of the aerial parts of Achyrocline flaccida (A. flaccida), Eupatorium arnottianum (E. arnottianum) and Eupatorioum buniifolium (E. buniifolium), leaves of Lithraea molleoides (L. molleoides) and leaves and stems of Phyllanthus sellowianus (P. sellowianus) were analyzed to disclose their effects on murine normal and tumor cell growth as well as on complement hemolytic activity. Modulation of cell growth was evaluated by tritiated thymidine incorporation while inhibition of complement activity was measured on both classical and alternative complement pathways (CP and AP respectively). The results obtained show that most of the extracts exerted inhibitory effect on tumor as well as on mitogen activated normal spleen cell growth. On tumor cells, IC50 ranged between 1-75 microg/ml for most of the extracts with the exception of dichloromethane of L. molleoides and P. sellowianus which required concentrations higher than 100 microg/ml to produce the effect. On mitogenic activated splenocytes, IC50 ranged between < 1 to 85 microg/ml with the exception of methanolic extract of E. buniifolium or P. sellowianus which were not effective on ConA or LPS stimulated splenocytes respectively. Only E. buniifolium was active on murine normal splenocytes proliferation (IC50 0.5-1.5 microg/ml). Finally, one (7%) of 15 extracts showed inhibition of complement activity on CP and 6 extracts (40%) presented moderate activity on CP. The dichloromethane extract of E. arnottianum was the most active (IC50 5 microg/ml), although remarkable effect was also obtained with dichloromethane and methanolic extracts of P. sellowianus (IC50 11.2 and 17.3 microg/ml respectively). Besides, 2 extracts (13%), dichloromethane extract of E. arnottianum and aqueous extract of P. sellowianus, showed moderate inhibition on AP.
Fernández
T
T
Cátedras de Inmunología/IDEHU, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina. terefern@ffyb.uba.ar
Cerdá Zolezzi
P
P
Risco
E
E
Martino
V
V
López
P
P
Clavin
M
M
Hnatyszyn
O
O
Canigueral
S
S
Hajos
S
S
Ferraro
G
G
Alvarez
E
E
D036921:Achyrocline; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D027926:Anacardiaceae; D000818:Animals; D001118:Argentina; D002455:Cell Division / Q000187:drug effects; D015941:Complement Hemolytic Activity Assay; D004912:Erythrocytes / Q000187:drug effects; D036602:Eupatorium; D005260:Female; D006801:Humans; D020128:Inhibitory Concentration 50; D019684:Magnoliopsida; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D029803:Phyllanthus; D010936:Plant Extracts / Q000494:pharmacology*; D011817:Rabbits; D012756:Sheep / Q000097:blood; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000276:Adjuvants, Immunologic; D010936:Plant Extracts