title: A novel quality control slide for quantitative immunohistochemistry testing.
abstract: We introduce a novel quality control technology that may improve intra- and interlaboratory immunohistochemistry (IHC) standardization. The technology involves the creation of standardized antibody targets that are attached to the same slides as the patient sample. After IHC staining, the targets turn the same color as the stained cells or tissue elements. Unlike current clinical practice, our proposed targets are neither cells nor tissue sections. To create reproducible standards that are available in unlimited supply, we use short constrained peptides as antibody targets. These peptides are attached directly to the glass slide. We show that these peptides simulate the portion of the native antigen to which the antibody binds. They are useful in detecting subtle changes in IHC staining efficacy. Moreover, the peptides do not degrade after deparaffinization or antigen retrieval treatments. This technology may be valuable in creating nationally standardized controls to quantify IHC analytical variability.
authors.affiliation: CytoLogix Corporation, Cambridge, Massachusetts 02139, USA. ssompuram@cytologix.com
authors.forename: Seshi R
authors.initials: SR
authors.lastname: Sompuram
authors.forename: Vani
authors.initials: V
authors.lastname: Kodela
authors.forename: Keming
authors.initials: K
authors.lastname: Zhang
authors.forename: Halasya
authors.initials: H
authors.lastname: Ramanathan
authors.forename: Gail
authors.initials: G
authors.lastname: Radcliffe
authors.forename: Peter
authors.initials: P
authors.lastname: Falb
authors.forename: Steven A
authors.initials: SA
authors.lastname: Bogen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D000939:Epitopes; D005898:Glass; D007150:Immunohistochemistry / Q000379:methods / Q000592:standards*; D051379:Mice; D018716:Molecular Mimicry; D009369:Neoplasms / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000592:standards; D011786:Quality Control; D011980:Receptors, Progesterone / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D010455:Peptides; D011980:Receptors, Progesterone