Influence of plasminogen deficiency on the contribution of polymorphonuclear leucocytes to fibrin/ogenolysis: studies in plasminogen knock-out mice.
Plasminogen knock-out (PG(-/-)) mice provide an unique opportunity for the study of alternative mediators of fibrinolysis. Polymorpho-nuclear leucocytes (PMNs) contain non-plasmin fibrinolytic proteases, however the degree to which these cells contribute to fibrin(ogen) degradation in these animals is not known. Thrombi were generated in carotid arteries and jugular veins of PG(-/-) and wild type (PG(+/+)) mice following adventitial application of a 20% ferric chloride solution. PMNs, identified histologically on H&E staining and by immunohistochemistry using anti-mouse PMN RB6-8C5 antibody, accumulated within the thrombus by 6 h after the injury and peaked at 24 h. There was significantly greater retention of PMNs within the thrombi of PG(-/-) mice from 48 to 72 h than in the PG(+/+) controls (at 72 h: PG(-/-)255 +/- 41 cell/mm(2) (n = 5), PG(+/+) 61 +/- 10 cell/mm(2) (n = 5), p<0.01 in the arterial thrombi; PG(-/-) 252 +/- 50 cell/mm(2) (n = 5), PG(+/+) 100 +/- 36 cell/mm(2) (n = 5), p<0.05 in the venous thrombi), providing potential for more PMN derived fibrinolytic enzymes to be present at late times after a thrombotic challenge in PG(-/-) mice relative to the PG(+/+) controls. Intact PMNs were elicited from the peritoneal cavities of PG(-/-) and PG(+/+) mice following 4% thioglycolate stimulation. In vitro studies showed PMNs from PG(-/-) mice to release greater quantities of 10% trichloroacetic acid (TCA)-soluble fibrinopeptides from I(125)-labeled fibrinogen, than cells from PG(+/+) controls although these differences did not become apparent until after 24 h of incubation (at 72 h incubation: PG(-/-) 918 n/10 x 10(6) cells/0.5 ml, PG(+/+) 589 ng/10 10(6) cells/0.5 ml p = 0.005). Furthermore, autoradiographic analysis of the I(125)-labeled fibrinogen degradation products showed the cleavage pattern by PG(-/-) PMNs to be distinct from that produced by PG(+/+) PMNs. These data suggest that a relatively greater role for PMNs-initiated fibrinolysis exists in the setting of plasminogen deficiency, although this prominence only becomes evident more than 24 h after the thrombotic insult. In addition, mechanisms responsible for the process in PG(-/-) mice may be distinct from those primarily responsible for the process in PMNs from PG(+/+) mice.
Zeng
Biao
B
Department of Cardiology, Concord Hospital, The University of Sydney, NSW, Australia.
Bruce
David
D
Kril
Jillian
J
Ploplis
Victoria
V
Freedman
Ben
B
Brieger
David
D
D000818:Animals; D005337:Fibrin / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism*; D005838:Genotype; D006651:Histocytochemistry; D007457:Iodine Radioisotopes; D007700:Kinetics; D051379:Mice; D018345:Mice, Knockout; D009504:Neutrophils / Q000502:physiology*; D010447:Peptide Hydrolases / Q000378:metabolism; D010958:Plasminogen / Q000172:deficiency* / Q000235:genetics; D013927:Thrombosis / Q000473:pathology*
D007457:Iodine Radioisotopes; D005337:Fibrin; D005340:Fibrinogen; D010958:Plasminogen; D010447:Peptide Hydrolases