An electrochemical ELISA procedure for the screening of 17beta-estradiol in urban waste waters.
A sensitive electrochemical enzyme-linked immunosorbent assay (ELISA) has been used for the detection of 17beta-estradiol in waste waters. The activity of the label enzyme (horseradish peroxidase) was measured electrochemically using 3,3',5,5'-tetramethylbenzidine as electrochemical substrate. The detection limit was estimated to be 5 pg mL(-1), interday and intraday precision (RSD), ranged from 1 to 3% and from 3 to 6%, respectively. Analysis of waste waters from three different treatment plants demonstrated no matrix effect both for samples diluted 1:1 in buffer and diethyl ether extracted. Data on 36 samples analysed by an LC-ESI-MS-MS procedure and by the electrochemical ELISA assay were compared. Results correlated well. The electrochemical enzyme immunoassay appears suitable as a screening tool for analysis of estradiol in waste waters.
Valentini
F
F
Dipartimento di Scienze e Tecnologie Chimiche, Università Tor Vergata, Rome, Italy. federica.valentini@uniroma2.it
Compagnone
D
D
Gentili
A
A
Palleschi
G
G
D004563:Electrochemistry / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004958:Estradiol / Q000032:analysis*; D017022:Flow Injection Analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*
D014874:Water Pollutants, Chemical; D004958:Estradiol