Studies on survival and outgrowth of processes of cultured rat hippocampus neurons in containing selenium and free serum medium.
OBJECTIVE
To study the effect of selenium on cultured newborn rat's hippocampus neurons survival and outgrowth development.


METHODS
Using the technique of primary culture of hippocampal neurons of newborn rat. The different dose of Se (62.5 microgram/L, 125.0 microgram/L, 182.5 microgram/L) were added into the medium at same time. We not only investigated the number of survival of neurons on 1 - 14 d and 1d to 10 d in with and without serum containing-Se medium, but also observed the length outgrowth of the neurite at 16 h, 24 h, 36 h, 48 h during culture.


RESULTS
Selenium could obviously enhance the outgrowth of early processed in 10% fetal serum medium and average length of neurite outgrowth is 15 - 20 micrometer more longer than control groups (P < 0.01) and selenium could also increase the livability of neurons and prolong survival time of cultured neurons in serum-free medium.


CONCLUSIONS
Selenium may play a very important role for early processed growth and development of hippocampal neurons.
Tian
Dongping
D
Department of Pathology, Shantou University Medical College, Guangdong 515031, China.
Su
Min
M
Song
Tianbao
T
Li
Guangyuan
G
Xu
Xiaohu
X
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D005260:Female; D006624:Hippocampus / Q000166:cytology*; D008297:Male; D016501:Neurites / Q000187:drug effects; D009474:Neurons / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000494:pharmacology*
D000975:Antioxidants; D016895:Culture Media, Serum-Free; D012643:Selenium