title: Characterization of a Saccharomyces cerevisiae NADP(H)-dependent alcohol dehydrogenase (ADHVII), a member of the cinnamyl alcohol dehydrogenase family.
abstract: A new NADP(H)-dependent alcohol dehydrogenase (the YCR105W gene product, ADHVII) has been identified in Saccharomyces cerevisiae. The enzyme has been purified to homogeneity and found to be a homodimer of 40 kDa subunits and a pI of 6.2-6.4. ADHVII shows a broad substrate specificity similar to the recently characterized ADHVI (64% identity), although they show some differences in kinetic properties. ADHVI and ADHVII are the only members of the cinnamyl alcohol dehydrogenase family in yeast. Simultaneous deletion of ADH6 and ADH7 was not lethal for the yeast. Both enzymes could participate in the synthesis of fusel alcohols, ligninolysis and NADP(H) homeostasis.
authors.affiliation: Department of Biochemistry and Molecular Biology, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Carol
authors.initials: C
authors.lastname: Larroy
authors.forename: Xavier
authors.initials: X
authors.lastname: Parés
authors.forename: Josep A
authors.initials: JA
authors.lastname: Biosca
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000737:chemistry; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D019281:Dimerization; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D017353:Gene Deletion; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D009154:Mutation; D009249:NADP / Q000378:metabolism; D020836:Protein Structure, Quaternary; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics*; D012984:Software; D013379:Substrate Specificity
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D009249:NADP; D000429:Alcohol Oxidoreductases; C470097:ADH7 protein, S cerevisiae; D000426:Alcohol Dehydrogenase; C018656:cinnamyl alcohol dehydrogenase; C446927:ADH6 protein, S cerevisiae