Quercetin, apoptosis, heat shock.
The present study was designed to investigate the correlation between the expression level of Hsp27 and Hsp72 and induction of apoptosis in HeLa cells in response to quercetin treatment. Treatment of HeLa cells with quercetin or with 1hr period of hyperthermia (42 degrees) increased the number of apoptotic cells. Inhibition of the expression of Hsp72 and Hsp27 in tumour cells by anti-sense oligonucleotides, enhanced the induction of apoptosis by quercetin. Heat shock itself had little effect on apoptotic cell death in these cells, but when combined with quercetin treatment, caused a significant increase in the number of apoptotic cells. These results suggest that the reduction of Hsps expression in the HeLa cell line promotes the induction of apoptosis by quercetin.
Jakubowicz-Gil
Joanna
J
Department of Comparative Anatomy and Anthropology, Maria Curie-Sklodowska University, ul. Akademicka 19, 20-033, Lublin, Poland. jjgil@biotop.umcs.lublin.pl
Rzymowska
Jolanta
J
Gawron
Antoni
A
D017209:Apoptosis; D002470:Cell Survival / Q000187:drug effects; D004347:Drug Interactions; D055551:HSP27 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins; D006367:HeLa Cells; D018882:Heat Stress Disorders / Q000473:pathology; D006360:Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D006979:Hyperthermia, Induced; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology; D011794:Quercetin / Q000494:pharmacology*; D014162:Transfection
D055551:HSP27 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D016376:Oligonucleotides, Antisense; D011794:Quercetin