title: Localization of a new highly repeated DNA sequence of Lemur cafta (Lemuridae, Strepsirhini).
abstract: We have isolated and cloned an 800-bp highly repeated DNA (HRDNA) sequence from Lemur catta (LCA) and described its localization on LCA chromosomes. Lemur catta HRDNA sequences were localized by performing FISH experiments on standard and elongated metaphasic chromosomes using an LCA HRDNA probe (LCASAT). A complex hybridization pattern was detected. A strong pericentromeric hybridization signal was observed on most LCA chromosomes. Chromosomes 7 and 13 were lit in pericentromeric regions, as well as in the interspersed heterochromatin. Chromosomes 1, 3, 4, 17, 19, X, and microchromosomes (20, 25, 26, and 27) showed no signals in the pericentromeric region, but chromosomes 3 and 4 showed a positive hybridization in heterochromatic regions. The 800-bp L catta HRDNA was species specific. We performed FISH experiments with the LCASAT probe on Eulemur macaco macaco (EMA) and Eulemur fulvus fulvus (EFU) metaphases and no positive signal of hybridization was detected. These findings were also confirmed by Southern blot analysis and PCR.
authors.affiliation: Università di Trieste, Dipartimento di Scienze della Riproduzione e dello Sviluppo, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Boniotto
authors.forename: Mario
authors.initials: M
authors.lastname: Ventura
authors.forename: Maria Francesca
authors.initials: MF
authors.lastname: Cardone
authors.forename: Francesca
authors.initials: F
authors.lastname: Boaretto
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Archidiacono
authors.forename: Mariano
authors.initials: M
authors.lastname: Rocchi
authors.forename: Sergio
authors.initials: S
authors.lastname: Crovella
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D006570:Heterochromatin / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D007900:Lemur / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D006570:Heterochromatin; D004247:DNA