Isolation and identification of an esterase from a Mexican strain of Boophilus microplus (Acari: Ixodidae).
A strain of Mexican Boophilus microplus (Cz) collected near Coatzacoalcos, Veracruz, Mexico, exhibits a moderate, but significant, level of permethrin resistance. Unlike other highly permethrin resistant strains, the Cz strain does not have a mutation within the sodium channel gene that results in target-site insensitivity. However, the Cz strain possesses a substantial increase in general and permethrin esterase activity relative to highly permethrin resistant and control strains suggesting the involvement of a metabolic esterase(s) in the expression of permethrin resistance. We report the isolation of a 62.8 kDa protein from Cz strain larvae that we think is the esterase previously reported as Cz EST9. In addition, internal amino acid sequence data obtained from the 62.8 kDa protein suggest that it is the gene product of a previously reported B. microplus carboxylesterase cDNA. We propose that the 62.8 kDa protein (Cz EST9) has permethrin hydrolytic activity and as a result plays an important role in Cz strain resistance to permethrin.
Pruett
J H
JH
Knipling-Bushland U. S. Livestock Insects Research Laboratory, USDA-ARS-SPA, Kerrville, TX 78028, USA. jhptlab@ktc.com
Guerrero
F D
FD
Hernandez
R
R
D000595:Amino Acid Sequence; D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004950:Esterases / Q000032:analysis*; D026863:Ixodidae / Q000201:enzymology*; D008800:Mexico; D008969:Molecular Sequence Data; D020539:Sequence Analysis, Protein; D012967:Sodium Dodecyl Sulfate
D012967:Sodium Dodecyl Sulfate; D004950:Esterases