Isolation and partial characterization of an antibacterial substance produced by Enterococcus faecium.
A strain of Enterococcus faecium isolated from Bulgarian yellow cheese "kashkaval" produced a bacteriocin-like substance named enterococcin A 2000. The antibacterial substance had a low molar mass (< 2 kDa), was relatively stable toward heat but was sensitive to selected proteolytic enzymes. It was active against Gram-positive bacteria including enterococci, such as Listeria, Bacillus and Streptococcus, and also against Gram-negative E. coli. Production of enterococcin A 2000 has a maximum near the end of the exponential phase of producer growth. The peptide was purified by ammonium sulfate precipitation, butanol extraction, followed by cation-exchange chromatography and reversed-phase chromatography. A partial sequence of purified enterococcin A 2000 indicated that this substance does not belong to the class IIa of bacteriocins presenting the consensus anti-Listeria motif YGNGV.
Pantev
A
A
Department of Microbiology, Faculty of Biology, University of Sofia, 1423 Sofia, Bulgaria.
Kabadjova
P
P
Dalgalarrondo
M
M
Haertlé
T
T
Ivanova
I
I
Dousset
X
X
Prévost
H
H
Chobert
J M
JM
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001430:Bacteriocins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D001483:Base Sequence; D002611:Cheese / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D016984:Enterococcus faecium / Q000737:chemistry* / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects; D008087:Listeria / Q000187:drug effects; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S