Prep2: cloning and expression of a new prep family member.
We describe Prep2, a new murine homeobox-containing gene closely related to Prep1. The PREP2 protein belongs to the three amino acid loop extension (TALE) superclass of homeodomain-containing proteins and encodes a polypeptide of 462 residues. As for PREP1, PREP2 binds an appropriate site on DNA as a heterodimer with PBX1A. Northern analysis, immunoblotting, immunohistochemistry, and in situ hybridization show widespread Prep2 expression during organogenesis and in the adult. The data suggest that Prep2 functions to varying degrees in a broad array of tissues and developmental processes.
Haller
Klaus
K
McGill Cancer Centre, McGill University, Montreal, Quebec, Canada.
Rambaldi
Isabel
I
Kovács
Erzsébet Nagy
EN
Daniels
Eugene
E
Featherstone
Mark
M
D000818:Animals; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D038081:Organogenesis / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*
D018398:Homeodomain Proteins; C486665:Pknox2 protein, mouse; D014157:Transcription Factors