Construction of BAC libraries derived from the ascidian Ciona intestinalis.
Large insert genomic bacterial artificial chromosome (BAC) libraries were constructed from a basal chordate, the ascidian Ciona intestinalis. Insert analyses of randomly selected clones indicated that in the first library the mean insert size was 135 kb and predicted a 15-fold coverage of the Ciona genome, and in the second library the mean insert size was 165 kb and predicted a 5-fold coverage of the genome. These first large insert genomic libraries of the ascidian should increase the speed of genomic analyses of basal chordates.
Kobayashi
Mari
M
Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Japan.
Matsuda
Masaru
M
Asakawa
Shuichi
S
Shimizu
Nobuyoshi
N
Nagahama
Yoshitaka
Y
Satou
Yutaka
Y
Satoh
Nori
N
D000818:Animals; D022202:Chromosomes, Artificial, Bacterial; D002938:Ciona intestinalis / Q000235:genetics*; D015723:Gene Library; D008297:Male; D013094:Spermatozoa / Q000378:metabolism