Separation of neutral saccharide mixtures with capillary electrochromatography using hydrophilic monolithic columns.
While developing a combination of capillary electrochromatography (CEC) with tandem mass spectrometry (MS) for the benefit of characterizing complex oligosaccharide mixtures, we needed highly efficient CEC columns operating in an "MS-friendly" mode. We demonstrate here novel types of polar, monolithic CEC columns that separate effectively complex mixtures of saccharides with the use of mobile phases containing acetonitrile/dilute ammonium formate buffers. Using the positive-ion mode of detection for neutral saccharides, the detection conditions were optimized down to the low-femtomole sensitivities with the use of an ion trap mass spectrometer. This column technology provides a nearly universal system that can separate a wide range of carbohydrates: mono- and oligosaccharides with the intact reducing end, as well as saccharide alditols. Even the anomers formed due to mutarotation could be resolved with a high content of organic phase.
Que
Amy H
AH
Department of Chemistry, Indiana University, Bloomington 47405, USA.
Novotny
Milos V
MV
D002241:Carbohydrates / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D007202:Indicators and Reagents; D036103:Nanotechnology; D021241:Spectrometry, Mass, Electrospray Ionization; D013402:Sugar Alcohols / Q000032:analysis
D002241:Carbohydrates; D007202:Indicators and Reagents; D013402:Sugar Alcohols