title: Pasteurized tumoral autograft as a novel procedure for limb sparing in the dog: A clinical report.
abstract: OBJECTIVE
  To evaluate use of a pasteurized tumoral autograft prepared from the resected primary bone neoplasm for limb sparing in a dog with distal radial osteosarcoma (OSA).
  
  
  METHODS
  Clinical case report.
  
  
  METHODS
  A 9-year-old male Maremma shepherd dog.
  
  
  METHODS
  After right distal radial OSA removal, the tumoral autograft was pasteurized. The excised bone segment was placed in a sterile watertight box containing sterile saline solution preheated to 65 degrees C in a water bath. The box was kept immersed in the water bath at 65 degrees C for 40 minutes to kill the tumor cells. The autograft was then fixed in the host with a plate and screws based on standard AO/ASIF technique for carpal arthrodesis. Three doses of cisplatin (70 mg/m(2) intravenously) were administered, 3 weeks apart; the initial dose was administered the day after surgery.
  
  
  RESULTS
  The autograft was incorporated in a manner comparable to an allograft, and after 708 days, the metallic implants were removed. A 1-month activity restriction as well as spoon splint to protect the leg from a full loading were used thereafter. Limb function was fair to good, and the dog remains disease free after 56 months.
  
  
  CONCLUSIONS
  A pasteurized autograft consisting of the resected primary bone neoplasm is a valid alternative to a cortical bone allograft for limb sparing in dogs with appendicular OSA in terms of feasibility and pattern of healing.
  
  
  CONCLUSIONS
  This procedure can be an alternative method of limb sparing when difficulties are encountered in establishing and maintaining a canine bone allograft bank.
authors.affiliation: Department of Animal Pathology, School of Veterinary Medicine, Turin, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Buracco
authors.forename: Emanuela
authors.initials: E
authors.lastname: Morello
authors.forename: Marina
authors.initials: M
authors.lastname: Martano
authors.forename: Maria Elisabetta
authors.initials: ME
authors.lastname: Vasconi
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000150:complications / Q000601:surgery / Q000662:veterinary*; D001863:Bone Screws / Q000662:veterinary; D016025:Bone Transplantation / Q000379:methods / Q000662:veterinary*; D004283:Dog Diseases / Q000000981:diagnostic imaging / Q000601:surgery*; D004285:Dogs; D007794:Lameness, Animal / Q000209:etiology; D008297:Male; D012516:Osteosarcoma / Q000150:complications / Q000601:surgery / Q000662:veterinary*; D011859:Radiography; D011884:Radius / Q000637:transplantation*; D014182:Transplantation, Autologous / Q000379:methods / Q000662:veterinary; D014945:Wound Healing