title: Correlative changes in metabolism and DNA damage in turbot (Scophthalmus maximus) exposed to benzo[a]pyrene.
abstract: Juvenile turbot (Scophthalmus maximus) were injected intraperitoneally with either corn oil or 5 mg/kg benzo[a]pyrene (BaP) dissolved in corn oil and sampled I and 3 days after injection. After 1 day, no elevation of 7-ethoxyresorufin O-deethylase (EROD) activity was observed, however bile metabolites (BaP-7,8 dihydrodiol representing 70% of the total metabolites) and a single hepatic DNA adduct spot (0.47 adducts/10(8) nucleotides) identified by 32P-postlabelling were formed. No BaP metabolites or DNA adducts were observed in either control or carrier control fish. Fish sampled after 3 days reported 5-fold higher (P < 0.05) levels of EROD activity, a shift in the bile metabolite profile towards BaP phenol formation (1OH and 30H BaP comprising up to 60% of total metabolites detected) and the formation of two adduct spots (0.86 and 0.71 adducts/10(8) nucleotides). These results show that BaP can be metabolised and form hydrophobic DNA adducts in turbot without EROD elevation. Following EROD elevation, a shift in the profile of both BaP metabolites and BaP metabolite-DNA interactions occurs indicative of other oxidative processes.
authors.affiliation: NERC Plymouth Marine Laboratory, Devon, UK. fatma.tellikarakoc@posta.mam.gov.tr
authors.forename: F
authors.initials: F
authors.lastname: Telli-Karakoç
authors.forename: P J
authors.initials: PJ
authors.lastname: Ruddock
authors.forename: D J
authors.initials: DJ
authors.lastname: Bird
authors.forename: A
authors.initials: A
authors.lastname: Hewer
authors.forename: A
authors.initials: A
authors.lastname: Van Schanke
authors.forename: D H
authors.initials: DH
authors.lastname: Phillips
authors.forename: L D
authors.initials: LD
authors.lastname: Peters
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000009:adverse effects*; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis* / Q000494:pharmacology; D018736:DNA Adducts; D004249:DNA Damage; D004790:Enzyme Induction; D005412:Flatfishes / Q000235:genetics* / Q000502:physiology; D007274:Injections, Intraperitoneal; D018384:Oxidative Stress; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
chemical_list: D018736:DNA Adducts; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene; D019363:Cytochrome P-450 CYP1A1