Purification and characterization of soluble peroxidase from oil palm (Elaeis guineensis Jacq) leaf.
Soluble peroxidase (POD) from oil palm leaf was purified by (NH(4))(2)SO(4) precipitation, anion exchange chromatography and molecular exclusion chromatography. The purification grade obtained was 429 yielding 54% of the enzyme activity. Electrophoresis of purified enzyme under denatured conditions revealed M(r) of 48+/-2 kDa. It has an optimum pH of 5 and it exhibited very high pH and thermal stabilities. K(m) for guaiacol, ABTS and pyrogallol were 3.96, 1 and 0.84 mM, respectively. Immunocytochemical localization studies showed that soluble POD was mainly located in the vascular bundles and epidermis of leaf.
Deepa
S S
SS
Agro Processing Division, Industrial Estate P O, Trivandrum 695 019, Kerala, India.
Arumughan
C
C
D000918:Antibody Specificity; D028023:Arecaceae / Q000201:enzymology*; D002241:Carbohydrates / Q000032:analysis; D004795:Enzyme Stability; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D008970:Molecular Weight; D010544:Peroxidases / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism*; D018515:Plant Leaves / Q000201:enzymology; D012995:Solubility; D013997:Time Factors
D002241:Carbohydrates; D010544:Peroxidases