Identification of an alternatively spliced isoform of the fyn tyrosine kinase.
Two isoforms of the src-family tyrosine kinase p59fyn have been shown to arise through alternative splicing of exon 7 of the fyn gene. These isoforms have been designated fynT, expressed in hematopoietic cells, and fynB, expressed in the brain. Here, we describe a novel isoform, fynDelta7, in which exon 7 is absent. FynDelta7 mRNA transcripts have been identified in peripheral blood mononuclear cells from all individuals tested thus far and semi-quantitative RT-PCR indicates that this fyn transcript is expressed at near wild-type levels. Transcripts coding for the fynDelta7 isoform can be detected in purified B cells, T cells, NK cells, and monocytes, indicating that it is not lineage specific. We further show that the message isolated is functional using an in vitro expression system and by its expression in COS cells.
Goldsmith
Jeffrey F
JF
Department of Pathology, University of Alabama-Birmingham School of Medicine, Birmingham, AL 35294, USA.
Hall
Craig G
CG
Atkinson
T Prescott
TP
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D017931:DNA Primers; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D019169:Jurkat Cells; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051076:Proto-Oncogene Proteins c-fyn; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020298:U937 Cells
D017931:DNA Primers; D007527:Isoenzymes; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C494957:FYN protein, human; D051076:Proto-Oncogene Proteins c-fyn