A study on cryopreservation of cultured rabbit periodontal ligament cells.
Cultured rabbit periodontal ligament cells were subjected to short term cryopreservation in liquid nitrogen, for a period of 84 hours and 168 hours, to study the effect of cryopreservation on coll viability and culturing ability. The vital cell count performed by Trypan Blue exclusion was 14.93 x 10(0) cells/ml in vial A and 9.11 x 10(6) cell/ml in vial B, before cryopreservation. The loss of viability was minimal--vital cell count being 14.64 x 10(6) cells/ml in vial A and 8.87 x 10(6) cells/ml in vial B after 84 hrs of cryopreservation and 14.6 x 10(6) cells/ml in vial A and 8.82 x 10(6) cells/ml in vial B after 168 hours of cryopreservation. The cryopreserved cells after thawing could grow again in cultured.
Pal
Tamal K
TK
Department of Periodontics, Dr. R. Ahmed Dental College, Kolkata, India. tamalpai@cal2.vsnl.net.in
Aibara
Farzan J
FJ
Ali
Nahid
N
D000818:Animals; D002448:Cell Adhesion; D002452:Cell Count; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D004396:Coloring Agents; D015925:Cryopreservation; D003470:Culture Media; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D008297:Male; D009584:Nitrogen; D010513:Periodontal Ligament / Q000166:cytology; D011817:Rabbits; D013997:Time Factors; D014343:Trypan Blue
D004396:Coloring Agents; D003470:Culture Media; D014343:Trypan Blue; D009584:Nitrogen