title: Cytochrome P450 differences in normal and imposex-affected female whelk Buccinum undatum from the open North Sea.
abstract: Normal and imposex-affected female Buccinum undatum were sampled from the open North Sea at three locations, one with low, and two with high shipping densities. Cytochrome P450 components and P450 aromatase activity were determined in the microsomal fractions isolated from pooled digestive gland/gonads. Cytochrome P450 aromatase activity was significantly higher (P < 0.05) in normal females collected in the low shipping density area (1,325 +/- 295 fmol/h/mg protein) than levels from imposex animals from a high shipping density area (620 +/- 287 fmol/h/mg protein). A negative correlation was found between aromatase activity and organotin body burden (r = -0.99). Levels of CYP450, cytochrome b5 and NADPH cytochrome c reductase activity did not show differences among groups. This is the first field evidence of depressed aromatase activity in imposex affected females, although additional research under laboratory controlled conditions is required to fully understand the mechanisms underlying the development of imposex in this species.
authors.affiliation: Department of Zoology and Anthropology, Faculty of Sciences, University of Porto, Portugal. mmsantos@fc.up.pt
authors.forename: M M
authors.initials: MM
authors.lastname: Santos
authors.forename: C C
authors.initials: CC
authors.lastname: ten Hallers-Tjabbes
authors.forename: N
authors.initials: N
authors.lastname: Vieira
authors.forename: J P
authors.initials: JP
authors.lastname: Boon
authors.forename: C
authors.initials: C
authors.lastname: Porte
mesh_terms: D000818:Animals; D001141:Aromatase / Q000494:pharmacology*; D001822:Body Burden; D012734:Disorders of Sex Development / Q000201:enzymology* / Q000662:veterinary*; D005260:Female; D008297:Male; D009251:NADPH-Ferrihemoprotein Reductase / Q000494:pharmacology*; D009947:Organotin Compounds / Q000009:adverse effects*; D012767:Ships; D012908:Snails / Q000502:physiology*; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
chemical_list: D009947:Organotin Compounds; D014874:Water Pollutants, Chemical; D001141:Aromatase; D009251:NADPH-Ferrihemoprotein Reductase