Motors and switches: AAA+ machines within the replisome.
Clamp loaders are required to load the ring-shaped clamps that tether replicative DNA polymerases onto DNA. Recently solved crystal structures, along with a series of biochemical studies, have provided a detailed understanding of the clamp loading reaction. In particular, studies of the Escherichia coli clamp loader--an AAA+ machine--have provided insights into the architecture of clamp loaders from eukaryotes, bacteriophage T4 and archaea. Other AAA+ proteins are also involved in the initiation of DNA replication, and studies of the E. coli clamp loader indicate mechanisms by which these proteins might function.
Davey
Megan J
MJ
Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.
Jeruzalmi
David
D
Kuriyan
John
J
O'Donnell
Mike
M
D001105:Archaea / Q000502:physiology; D001426:Bacterial Proteins / Q000502:physiology; D001435:Bacteriophages / Q000502:physiology; D004261:DNA Replication / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000502:physiology*; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000502:physiology; D004926:Escherichia coli / Q000502:physiology; D005057:Eukaryotic Cells / Q000502:physiology; D006801:Humans; D020409:Molecular Motor Proteins / Q000502:physiology*; D051738:Origin Recognition Complex; D017434:Protein Structure, Tertiary
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; D020409:Molecular Motor Proteins; D051738:Origin Recognition Complex; D004259:DNA-Directed DNA Polymerase