Protein expression signatures: an application of proteomics.
The methods of proteomics, the study of the protein complement of the genome, are applicable to environmental testing. Sets of proteins specific to different stressors can be isolated using computer imaging software. Individual proteins can be identified by mass spectrometry. The Protein Expression Signatures (PES) obtained have potential in diagnosing adverse factors in the environment. The challenge is to demonstrate their feasibility in complex environments. We have shown that PES for three endocrine disrupting compounds in trout (Onchorhynchus mykiss), can be detected in mixed sewage effluent. Other studies support these results. As protein databases expand, identification becomes routine, and capture molecules specific to each protein are developed, the possibility of simple field tests for multiple stressors becomes real.
Bradley
Brian P
BP
Department of Biological Sciences, University of Maryland, UMBC, Baltimore 21250, USA. bbradley@umbc.edu
Shrader
Elizabeth A
EA
Kimmel
David G
DG
Meiller
Jessica C
JC
D000818:Animals; D004703:Endocrine System / Q000187:drug effects; D004784:Environmental Monitoring / Q000379:methods; D004785:Environmental Pollutants / Q000009:adverse effects*; D005786:Gene Expression Regulation; D013058:Mass Spectrometry; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification; D040901:Proteomics; D012722:Sewage; D012984:Software; D014337:Trout / Q000235:genetics / Q000502:physiology
D004785:Environmental Pollutants; D011506:Proteins; D012722:Sewage