title: Rat hindlimb unloading: Soleus and Extensor Digitorum Longus histochemistry, mitochondrial DNA content and mitochondrial DNA deletions.
abstract: Mitochondrial phenotypic alterations, mitochondrial DNA content and mitochondrial DNA deletions in a slow, Soleus, and a fast, Extensor Digitorum Longus, skeletal muscle of 3- and 15-month-old hindlimb suspended rats have been studied. Cytochrome c oxidase-negative fibers appeared after unloading in all examined animals and their percentage increased with increasing unloading time. After 14 days of suspension the mitochondrial DNA content did not change in 3-month-old but decreased significantly in 15-month-old rats. Soleus was much more affected by unloading than Extensor Digitorum Longus. The mitochondrial DNA deletion of 4834 bp as well as other mtDNA deletions, researched with Long Distance-PCR, were absent in both studied muscles before and after unloading.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bari, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Pesce
authors.forename: A
authors.initials: A
authors.lastname: Cormio
authors.forename: F
authors.initials: F
authors.lastname: Fracasso
authors.forename: A M S
authors.initials: AM
authors.lastname: Lezza
authors.forename: P
authors.initials: P
authors.lastname: Cantatore
authors.forename: M N
authors.initials: MN
authors.lastname: Gadaleta
mesh_terms: D000818:Animals; D001284:Atrophy; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000235:genetics*; D017353:Gene Deletion; D019417:Hindlimb Suspension / Q000502:physiology*; D006651:Histocytochemistry; D018482:Muscle, Skeletal / Q000737:chemistry / Q000473:pathology / Q000502:physiology*; D051381:Rats
chemical_list: D004272:DNA, Mitochondrial