Production of hybrid double- or triple-layered virus-like particles of group A and C rotaviruses using a baculovirus expression system.
Dual infections by group A and group C rotaviruses have been reported, but no reassortants between group A and group C rotaviruses have been described. The VP6 major inner capsid protein of group A and C rotaviruses shares common antigens detected by monoclonal antibodies and also shares 40-43% amino acid identity. Coinfection of Spodoptera frugiperda (Sf9) insect cells with different combinations of the recombinant baculoviruses encoding either group A [RF VP2 (A-VP2), IND VP6 (A-VP6), and VP7 (A-VP7[IND]), 2292B VP7 (A-VP7[2292B])] or C [Shintoku VP6 (C-VP6) and VP7 (C-VP7)] bovine rotavirus proteins produced hybrid group A/C triple-layered VP2/6/7 virus-like particles (TLPs) composed of A-VP2/C-VP6/C-VP7, A-VP2/C-VP6/A-VP7(IND), A-VP2/C-VP6/A-VP7(2292B), and A-VP2/A-VP6/C-VP7. To our knowledge, this is the first report that the inner capsid VP6 of group A or group C rotavirus can support attachment of the heterologous, antigenically distinct group A (G6, IND or G10, 2292B) or group C rotavirus outer capsid VP7 to produce hybrid TLPs in vitro.
Kim
Yunjeong
Y
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster 44691, USA.
Chang
Kyeong Ok
KO
Kim
Won Yong
WY
Saif
Linda J
LJ
D000818:Animals; D000956:Antigens, Viral; D016367:Baculoviridae; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D005822:Genetic Vectors; D006801:Humans; D008253:Macaca mulatta; D008854:Microscopy, Electron / Q000379:methods; D012401:Rotavirus / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D018411:Spodoptera; D014771:Virion / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D019065:Virus Assembly / Q000502:physiology*
D000956:Antigens, Viral; D036022:Capsid Proteins; C442545:VP2 protein, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus