title: Comparison of tissue culture and animal models for assessment of Cryptospridium parvum infection.
abstract: The current increased interest for using tissue culture as a surrogate for mouse infection to assess Cryptospridium viability suggests that a comparison of the two models is essential for data interpretation. Therefore, a need remains for a statistical comparison that can demonstrate if infection and inactivation predicted by new tissue culture models are comparable with those predicted by animal models. Data from a total of 31 dose-response trials using both tissue culture and mouse models to assess C. parvum infectivity were compared. The dose needed to infect 50% of the tissue cultures (ID(50)) was also compared to each ID(50) in mice. Average ID(50)s developed using the logit dose-response method for tissue culture and mice were 8 and 107, respectively, suggesting that tissue culture was more sensitive to infection. However, correlation (r) between tissue culture and mouse infectivity was statistically significant (0.9167 [95% CI=0.8428 to 0.9594, p<0.0001]). Comparison of oocyst disinfection by UV and chlorine dioxide showed no significant difference between inactivation predicted by tissue culture and mouse models (p=0.8893; t=0.0141; n=21). These results demonstrate that tissue culture can successfully be used to measure C. parvum infection and can be used for determining inactivation in disinfection studies.
authors.affiliation: Orange County Utilities Laboratory, Orlando, FL 32825, USA. terri.slifko@ocfl.net
authors.forename: Theresa R
authors.initials: TR
authors.lastname: Slifko
authors.forename: Debra E
authors.initials: DE
authors.lastname: Huffman
authors.forename: Bertrand
authors.initials: B
authors.lastname: Dussert
authors.forename: James H
authors.initials: JH
authors.lastname: Owens
authors.forename: Walter
authors.initials: W
authors.lastname: Jakubowski
authors.forename: Charles N
authors.initials: CN
authors.lastname: Haas
authors.forename: Joan B
authors.initials: JB
authors.lastname: Rose
mesh_terms: D000230:Adenocarcinoma; D000818:Animals; D000831:Animals, Newborn; D003457:Cryptosporidiosis / Q000469:parasitology*; D016785:Cryptosporidium parvum / Q000502:physiology*; D004195:Disease Models, Animal; D006801:Humans; D007078:Ileal Neoplasms; D007080:Ileocecal Valve; D051379:Mice; D008807:Mice, Inbred BALB C; D012044:Regression Analysis; D014407:Tumor Cells, Cultured