Choline-binding protein A of Streptococcus pneumoniae elicits chemokine production and expression of intercellular adhesion molecule 1 (CD54) by human alveolar epithelial cells.
Recruitment of neutrophils into alveolar air spaces is an early event in the pathogenesis of pneumonia due to Streptococcus pneumoniae. This results from chemokines released by activated endothelial and epithelial cells and alveolar macrophages. Culture supernatants of 6 wild-type strains of S. pneumoniae, shown to contain choline-binding protein A (CbpA; clades A and B), induced release of chemokine CXCL8 from the human alveolar epithelial cell line A549, whereas a CbpA deletion mutant elicited significantly reduced CXCL8 release, compared with that of its isogenic parent (P<.01). Recombinant CbpA up-regulated expression of messenger RNA of CXCL8 and CCL2 but not of XCL1, CXCL10, CCL1, CCL3, CCL4, or CCL5 in A549 cells and induced increased secretion of CXCL8, CCL2, CXCL1, and CXCL5 in a dose- and time-dependent manner. CbpA also increased the expression of intercellular adhesion molecule 1 (CD54) by A549 cells. Thus, CbpA of S. pneumoniae induces the transcription and release of proinflammatory molecules by human alveolar epithelial cells.
Murdoch
Craig
C
Division of Genomic Medicine, University of Sheffield, Sheffield, United Kingdom.
Read
Robert C
RC
Zhang
Qibo
Q
Finn
Adam
A
D000942:Antigens, Bacterial / Q000276:immunology / Q000494:pharmacology*; D001483:Base Sequence; D002460:Cell Line; D018925:Chemokines / Q000235:genetics*; D017931:DNA Primers; D004847:Epithelial Cells / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011650:Pulmonary Alveoli / Q000276:immunology*; D019707:Receptors, Chemokine / Q000235:genetics*; D013296:Streptococcus pneumoniae / Q000276:immunology*; D014158:Transcription, Genetic / Q000187:drug effects
D000942:Antigens, Bacterial; D018925:Chemokines; D017931:DNA Primers; D019707:Receptors, Chemokine; D018799:Intercellular Adhesion Molecule-1