Binding of Norwalk virus-like particles to ABH histo-blood group antigens is blocked by antisera from infected human volunteers or experimentally vaccinated mice.
Attachment of Norwalk (NV), Snow Mountain (SMV), and Hawaii (HV) virus-like particles (VLPs) to specific ABH histo-blood group antigens was investigated by using human saliva and synthetic biotinylated carbohydrates. The three distinct Norwalk-like viruses (NLVs) have various capacities for binding ABH histo-blood group antigens, suggesting that different mechanisms for NLV attachment likely exist. Importantly, antisera from NV-infected human volunteers, as well as from mice inoculated with packaged Venezuelan equine encephalitis virus replicons expressing NV VLPs, blocked the ability of NV VLPs to bind synthetic H type 1, Le(b), and H type 3, suggesting a potential mechanism for antibody-mediated neutralization of NV.
Harrington
Patrick R
PR
Department of Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7435, USA.
Lindesmith
Lisa
L
Yount
Boyd
B
Moe
Christine L
CL
Baric
Ralph S
RS
D000017:ABO Blood-Group System / Q000378:metabolism*; D000818:Animals; D000914:Antibodies, Viral / Q000378:metabolism*; D001665:Binding Sites; D017250:Caliciviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D002460:Cell Line; D006224:Cricetinae; D004666:Encephalitis Virus, Venezuelan Equine / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D009500:Neutralization Tests; D029322:Norovirus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D009663:Norwalk virus / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D012093:Replicon; D012463:Saliva / Q000276:immunology / Q000821:virology; D014611:Vaccination; D014765:Viral Vaccines / Q000494:pharmacology
D000017:ABO Blood-Group System; D000914:Antibodies, Viral; D014765:Viral Vaccines