Microcystin-LR alters the growth, anthocyanin content and single-stranded DNase enzyme activities in Sinapis alba L seedlings.
Seedlings of the white mustard (Sinapis alba L.) are sensitive to the cell-free extracts of a toxigenic strain of Microcystis aeruginosa and to microcystin-LR. Fresh mass of plants, plant length, including hypocotyl and root length and lateral root formation is inhibited in microcystin-LR treated seedlings. The decrease of anthocyanin content is obtained in microcystin treated mustard cotyledons. The tissue necrosis of cotyledons is a characteristic consequence of microcystin treatment. Microcystin-LR induces an increase in single stranded deoxyribonucleases (ssDNases) activity of S. alba seedlings as shown by spectrophotometric assays and by ssDNase activity polyacrylamide gels. The significance of this phenomenon is discussed in relation to general stress responses in plants. We conclude that microcystin-LR affects the whole physiology and the growth of plants.
M-Hamvas
Márta
M
Department of Botany, The University of Debrecen, H-4010, P O Box 14, Debrecen, Hungary.
Máthé
Csaba
C
Molnár
Erika
E
Vasas
Gábor
G
Grigorszky
István
I
Borbely
George
G
D000872:Anthocyanins / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic / Q000494:pharmacology*; D018517:Plant Roots / Q000201:enzymology / Q000254:growth & development / Q000502:physiology; D036226:Seedlings / Q000201:enzymology / Q000254:growth & development; D031226:Sinapis / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D013053:Spectrophotometry
D000872:Anthocyanins; D004791:Enzyme Inhibitors; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; D003851:Deoxyribonucleases; C057862:cyanoginosin LR