Establishment of a triploid V79 cell line from tetraploid cells obtained through polyploidization using K-252a.
Triploid V79 cells were established from tetraploid cells. Diploid V79 cells were polyploidized by K-252a, an inhibitor of protein kinases, and then released from the drug for 10 days. At that time, the cell population was a mixture of diploid and tetraploid cells. Triploid cells were obtained through the cloning of tetraploid cells. They had 33 chromosomes (1.5 times the diploid number) and showed a karyotype of three homologueous chromosomes. The duration of the G1, S and G2/M phases was almost the same as for diploid cells. The cell volume of triploid V79 cells was about two times that of the diploid cells. An explanation for the diploid-tetraploid-triploid transition is proposed.
Fujikawa-Yamamoto
Kohzaburo
K
Division of Basic Science, Research Institute of Medical Science, Kanazawa Medical University, Ishikawa, Japan. fujikawa@kanazawa-med.ac.jp
Yamagishi
Hiroko
H
Miyagoshi
Minoru
M
D000818:Animals; D002227:Carbazoles / Q000494:pharmacology*; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D048429:Cell Size; D002875:Chromosomes / Q000648:ultrastructure; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D004171:Diploidy; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D026121:Indole Alkaloids; D007621:Karyotyping; D007700:Kinetics; D008957:Models, Genetic; D011123:Polyploidy
D002227:Carbazoles; D004791:Enzyme Inhibitors; D026121:Indole Alkaloids; D004247:DNA; C049985:staurosporine aglycone