title: Ultrastructural studies of experimental scrapie and Creutzfeldt-Jakob disease in hamsters. II. Astrocytic and macrophage reaction towards axonal destruction.
abstract: We report here the microglial (macrophage) and astrocytic reaction in several models of transmissible spongiform encephalopathies (TSEs) or prion diseases. With the low power electron microscopy it was readily apparent that myelinated vacuoles were surrounded by cells and their processes. The latter belonged either to hyperplastic reactive astrocytes or to macrophages. Typically, reactive astrocytes exhibited cytoplasm filled with innumerable glial filaments and, occasionally, other organelles (like cilia) and abundant tortuous intercellular junctions of adhesive plaque junction type. Desmosome-like junctions connecting astrocytic elements were also seen. As described earlier, astrocytic processes were occasionally interdigitated with oligodendroglial cells and their processes. Two types of macrophages were readily described. The majority of them exhibited electron-dense cytoplasm and numerous "empty" vacuoles (digestive chambers) containing cellular debris. Occasional vacuoles were surrounded by a thin collar reminiscent of "lyre-like inclusions" of the second type of macrophages. Several mylinated fibres were clearly engulfed by the cytoplasm of a macrophage containing unusual annulate lamellae.
authors.affiliation: Laboratory of Electron Microscopy and Neuropathology, Department of Molecular Biology, Medical Academy Łódź, Poland. ppliber@csk.am.lodz.pl.
authors.forename: Paweł P
authors.initials: PP
authors.lastname: Liberski
authors.forename: Jolanta
authors.initials: J
authors.lastname: Bratosiewicz-Wasik
authors.forename: D Carleton
authors.initials: DC
authors.lastname: Gajdusek
authors.forename: Paul
authors.initials: P
authors.lastname: Brown
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000473:pathology*; D001369:Axons / Q000473:pathology* / Q000648:ultrastructure; D001921:Brain / Q000473:pathology / Q000648:ultrastructure; D007562:Creutzfeldt-Jakob Syndrome / Q000473:pathology*; D006224:Cricetinae; D008264:Macrophages / Q000473:pathology* / Q000648:ultrastructure; D008647:Mesocricetus; D008854:Microscopy, Electron; D009413:Nerve Fibers, Myelinated / Q000473:pathology / Q000648:ultrastructure; D012608:Scrapie / Q000473:pathology*; D014617:Vacuoles / Q000648:ultrastructure; D014855:Wallerian Degeneration / Q000473:pathology