Colicin A multimerizes when unfolded.
A purified preparation of colicin A produced by Escherichia coli cells contained various forms of colicin A. Unfolding of the purified colicin A with urea provoked multimerization. Dimers, tetramers and hexamers of colicin A were identified.
Cavard
Danièle
D
LISM-CNRS, 31, chemin Joseph-Aiguier, 13402 Marseille cedex 20, France. cavard@ibsm.cnrs-mrs.fr
D003087:Colicins / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D011108:Polymers / Q000378:metabolism*; D017510:Protein Folding; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D003087:Colicins; D011108:Polymers