Attenuation of spontaneous opiate withdrawal in mice by the anandamide transport inhibitor AM404.
The endogenous cannabinoid, anandamide, has been shown to attenuate naloxone-precipitated opiate withdrawal in rodents. Here we show that the spontaneous, but not the naloxone-precipitated withdrawal syndrome in morphine-dependent mice is attenuated by the inhibitor of carrier-mediated anandamide transport N-(4-hydroxyphenyl) arachidonylethanolamide (AM404) (2 and 10 mg/kg, i.p.). These results suggest that spontaneous but not opioid antagonist-precipitated withdrawal is associated with dynamic changes in endogenous cannabinoid signaling.
Del Arco
Ignacio
I
Fundación Hospital Carlos Haya, Avda Carlos Haya 82. 7 Planta, Pabellón A., Málaga 29010, Spain.
Navarro
Miguel
M
Bilbao
Ainhoa
A
Ferrer
Belén
B
Piomelli
Daniele
D
Rodríguez De Fonseca
Fernando
F
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism* / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D063388:Endocannabinoids; D051379:Mice; D009021:Morphine Dependence / Q000378:metabolism*; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D053284:Polyunsaturated Alkamides; D013375:Substance Withdrawal Syndrome / Q000188:drug therapy* / Q000378:metabolism
D001095:Arachidonic Acids; D063388:Endocannabinoids; D009292:Narcotic Antagonists; D053284:Polyunsaturated Alkamides; D009270:Naloxone; C078814:anandamide; C107594:N-(4-hydroxyphenyl)arachidonylamide