Regulation of the production of polygalacturonase by Aspergillus niger.
Synthesis of ethylene in static cultures as well as the effect of endogenous and exogenous ethylene on the synthesis of polygalacturonase by Aspergillus niger were determined. This strain produced maximum ethylene amounts when cultured at 30 degrees C for 3 d. The effect of adding ethylene precursors (citrate-cycle intermediates) on ethylene production was investigated. Best intracellular and extracellular polygalacturonase production was obtained with 2-oxoglutaric, pyruvic and fumaric acids, and with glutamic acid too. Addition of ethylene to the culture medium also increased the synthesis of polygalacturonase, although to a lower degree than when glutamic acid was added.
Maldonado
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Department of Industrial Microbiology, Faculty of Biochemistry and Pharmacy, National University of Tucumán, Ayacucho 491, 4000 Tucumán, Argentina.
Cáceres
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Galli
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Navarro
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D001234:Aspergillus niger / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D018533:Biomass; D002952:Citric Acid Cycle; D003470:Culture Media; D005030:Ethylenes / Q000096:biosynthesis / Q000494:pharmacology; D005650:Fumarates / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology; D007656:Ketoglutaric Acids / Q000494:pharmacology; D007700:Kinetics; D011096:Polygalacturonase / Q000096:biosynthesis*; D019289:Pyruvic Acid / Q000494:pharmacology
D003470:Culture Media; D005030:Ethylenes; D005650:Fumarates; D007656:Ketoglutaric Acids; D018698:Glutamic Acid; D019289:Pyruvic Acid; C032005:fumaric acid; C036216:ethylene; D011096:Polygalacturonase