Ecophysiological variabilities in ectohydrolytic enzyme activities of some Pseudoalteromonas species, P. citrea, P. issachenkonii, and P. nigrifaciens.
The ecophysiological variabilities in the ectohydrolytic enzyme profiles of the three species of Pseudoalteromonas, P. citrea, P. issachenkonii, and P. nigrifaciens, have been investigated. Forty-one bacteria isolated from several invertebrates, macroalgae, sea grass, and the surrounding water exhibited different patterns of hydrolytic enzyme activities measured as the hydrolysis of either native biopolymers or fluorogenic substrates. The activities of the following enzymes were assayed: proteinase, tyrosinase, lipase, amylase, chitinase, agarase, fucoidan hydrolase, laminaranase, alginase, pustulanase, cellulase, beta-glucosidase, alpha- and beta-galactosidases, beta-N-acetylglucosaminidase, beta-glucosaminidase, beta-xylosidase, and alpha-mannosidase. The occurrence and cell-specific activities of all enzymes varied over a broad range (from 0 to 44 micromol EU per hour) and depended not only on taxonomic affiliation of the strain, but also on the source/place of its isolation. This suggests 'specialization' of different species for different types of polymeric substrates as, for example, all strains of P. citrea and P. issachenkonii hydrolyzed alginate and laminaran, while strains of P. nigrifaciens were lacking the ability to hydrolyze most of the algal polysaccharides. The incidence of certain enzymes such as fucoidan hydrolases, alginate lyases, agarases, and alpha-galactosidases might be strain specific and reflect its particular ecological habitat.
Ivanova
Elena P
EP
Industrial Research Institute Swinburne, Swinburne University of Technology, Hawthorn, Vic 3122, Australia. eivanova@swin.edu.au
Bakunina
I Yu
IY
Nedashkovskaya
Olga I
OI
Gorshkova
Nataliya M
NM
Alexeeva
Yulia V
YV
Zelepuga
Elena A
EA
Zvaygintseva
T N
TN
Nicolau
Dan V
DV
Mikhailov
V V
VV
D003470:Culture Media; D017753:Ecosystem; D006867:Hydrolases / Q000378:metabolism; D031161:Pseudoalteromonas / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D012623:Seawater
D003470:Culture Media; D006867:Hydrolases