title: Construction of BAC libraries derived from the ascidian Ciona intestinalis.
abstract: Large insert genomic bacterial artificial chromosome (BAC) libraries were constructed from a basal chordate, the ascidian Ciona intestinalis. Insert analyses of randomly selected clones indicated that in the first library the mean insert size was 135 kb and predicted a 15-fold coverage of the Ciona genome, and in the second library the mean insert size was 165 kb and predicted a 5-fold coverage of the genome. These first large insert genomic libraries of the ascidian should increase the speed of genomic analyses of basal chordates.
authors.affiliation: Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Masaru
authors.initials: M
authors.lastname: Matsuda
authors.forename: Shuichi
authors.initials: S
authors.lastname: Asakawa
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Shimizu
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Nagahama
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Satou
authors.forename: Nori
authors.initials: N
authors.lastname: Satoh
mesh_terms: D000818:Animals; D022202:Chromosomes, Artificial, Bacterial; D002938:Ciona intestinalis / Q000235:genetics*; D015723:Gene Library; D008297:Male; D013094:Spermatozoa / Q000378:metabolism