Quantification of hepatitis C virus in human liver and serum samples by using LightCycler reverse transcriptase PCR.
A highly sensitive, non-probe-based, real-time quantitative reverse transcriptase PCR was developed for viral load measurements in both serum and liver samples from patients with hepatitis C virus (HCV) infection. With synthetic RNA, the linearity of the approach was conserved over a wide range of HCV copy numbers. There was a strong correlation between hepatic and serum viral load measurements (r = 0.689, P = 0.004, n = 15), indicating that the level of viremia reflected the amount of virus present in the liver.
White
Peter A
PA
Virology Division, Department of Microbiology, SEALS, Prince of Wales Hospital, Sydney, New South Wales 2031, Australia. withepa@sesahs.nsw.gov.au
Pan
Yong
Y
Freeman
Anthony J
AJ
Marinos
George
G
Ffrench
Rosemary A
RA
Lloyd
Andrew R
AR
Rawlinson
William D
WD
D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D019698:Hepatitis C, Chronic / Q000821:virology*; D006801:Humans; D008099:Liver / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis* / Q000097:blood*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D019562:Viral Load; D014766:Viremia / Q000821:virology
D012367:RNA, Viral