Rapid time-resolved immunofluorometric assay for the measurement of creatine kinase in serum and whole blood samples.
A rapid and simple immunochemical method was developed for the assessment of the creatine kinase (MM) isoenzyme [CK(MM)], a protein marker linked with animal welfare and meat quality. The one-step time-resolved immunofluorometric assay produced quantitative results from serum or whole blood samples in 20 min. The analytical limit of detection (mean + 2s) for the immunoassay was 17 ng/mL (n = 6), and the functional limit of detection for the analysis of porcine whole blood samples was 426 ng/mL (n = 24). The working range of the method was linear up to 50 micro g/mL, and the within-assay precision varied between 2.1 and 10.9%. The analysis of porcine serum samples showed that the results from the immunoassay method and colorimetric CK enzyme activity determination were highly correlated (r(2) = 0.965, n = 17, p < 0.001). The practicability of the assay was demonstrated by the analysis of 300 porcine whole blood samples in a slaughterhouse environment.
Tuomola
Mika
M
Department of Biotechnology, University of Turku, FIN-20520 Turku, Finland. mika.tuomola@utu.fi
Vainio
Jani
J
Lövgren
Timo
T
D000818:Animals; D019921:Biotinylation; D003124:Colorimetry; D003402:Creatine Kinase / Q000097:blood*; D052283:Creatine Kinase, MM Form; D004591:Electrophoresis, Polyacrylamide Gel; D015200:Fluoroimmunoassay; D007527:Isoenzymes / Q000097:blood*; D008460:Meat; D018482:Muscle, Skeletal / Q000201:enzymology; D011786:Quality Control; D013552:Swine / Q000097:blood
D007527:Isoenzymes; D003402:Creatine Kinase; D052283:Creatine Kinase, MM Form