Lipid peroxidation stimulated by Solvesso, Bawanol and methanol, and its counteraction by antioxidants in human placental mitochondria.
The influence of some solvents used in the paint and lacquer industry, Solvesso 100, 150 and Bawanol, on the process of lipid peroxidation was evaluated. The interactions of the solvents with methanol and the antioxidative effect of coenzyme Q(10) (CoQ(10)) and vitamin E were also examined. The mitochondria isolated from human placenta were used as a model for investigation. The level of peroxidation was evaluated by malonodialdehyde (MDA) measurement. It was observed that Solvesso 150, Bawanol and methanol caused a statistically significant increase of MDA concentration and that the examined solvents had an antagonistic interaction with methanol. Solvesso 100 does not influence on MDA concentration but gives the same type of interaction with methanol as Solvesso 150. CoQ(10) and vitamin E proved to be effective antioxidants to counteract oxidative stress caused by the studied solvents, lowering lipid peroxidation expressed by MDA concentration.
Długosz
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Department of Toxicology, Wroclaw Medical University, 54-117 Wroclaw, Traugutta 57/59, Poland. amtox@polbox.com
Piotrowska
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D
D000975:Antioxidants / Q000494:pharmacology; D018929:Cell Culture Techniques; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000032:analysis; D000432:Methanol / Q000009:adverse effects*; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D010920:Placenta / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D012997:Solvents / Q000009:adverse effects*; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D012997:Solvents; D014810:Vitamin E; D008315:Malondialdehyde; D000432:Methanol