Nitric oxide synthase: use of stopped-flow spectroscopy and rapid-quench methods in single-turnover conditions to examine formation and reactions of heme-O2 intermediate in early catalysis.
Wei
Chin-Chuan
CC
Department of Immunology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA.
Wang
Zhi-Qiang
ZQ
Stuehr
Dennis J
DJ
D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001894:Borohydrides / Q000737:chemistry; D002384:Catalysis; D019281:Dimerization; D004227:Dithionite / Q000737:chemistry; D006900:Hydroxylation; D007501:Iron / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000737:chemistry / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D013057:Spectrum Analysis / Q000379:methods*
D001894:Borohydrides; D004227:Dithionite; D009569:Nitric Oxide; D001120:Arginine; D007501:Iron; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D010100:Oxygen