title: Thrombospondin-1 expression as a prognostic predictor of pancreatic ductal carcinoma.
abstract: Pancreatic cancer is a malignant tumor with an extremely poor prognosis. Thrombospondin-1 (TSP-1) was suggested to be a potential regulator of tumor growth and metastasis. We examined TSP-1 expression in 77 cases of invasive ductal adenocarcinoma of the pancreas, and analyzed the correlation between the TSP-1 expression pattern and clinicopathological features in pancreatic cancer. TSP-1 immunoreactivity was detected in the cancer stroma. The diffusely positive and focally positive patterns of TSP-1 were found in 33 (42.9%) and 40 (51.9%) of 77 cases, respectively. The TSP-1 diffuse expression was significantly correlated with lymph node metastasis (p<0.01), neural invasion (p<0.05) and TNM stage (p<0.01). The prognostic significance of clinicopathological parameters were analyzed by univariate and multivariate analysis using the log-rank test and the Cox proportional hazards model. Based on the univariate analysis, histological differentiation (p<0.01), lymphatic invasion (p<0.01), venous invasion (p<0.05), neural invasion (p<0.01), TNM stage (p<0.01) and TSP-1 expression (p<0.01) were significant parameters. These observations suggested that TSP-1 plays important roles in cancer cell growth and metastasis of human pancreatic cancer, and that stromal TSP-1 immunoreactivity is a good prognostic predictor of patients with pancreatic cancer.
authors.affiliation: Department of Surgery, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tobita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kijima
authors.forename: Shoichi
authors.initials: S
authors.lastname: Dowaki
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Oida
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kashiwagi
authors.forename: Masanori
authors.initials: M
authors.lastname: Ishii
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Sugio
authors.forename: Takafumi
authors.initials: T
authors.lastname: Sekka
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ohtani
authors.forename: Makiko
authors.initials: M
authors.lastname: Tanaka
authors.forename: Sadaki
authors.initials: S
authors.lastname: Inokuchi
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Makuuchi
mesh_terms: D021441:Carcinoma, Pancreatic Ductal / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D019700:Thrombospondin 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D019700:Thrombospondin 1