title: Integration of a membrane-based desalting step in a microfabricated disposable polymer injector for mass spectrometric protein analysis.
abstract: A simple desalting procedure for the coupling of a polymer microchip injector to mass spectrometry is proposed. The overall process is based on the adsorption of proteins on a poly(vinylidene difluoride) (PVDF) membrane, which are then directly eluted in the spraying solution. This microchip-based approach has been successfully applied to small drugs, peptides and proteins originally diluted in phosphate-buffered saline (PBS). Moreover, when eluting the retained proteins in small volumes, a preconcentration is obtained. The combination of single-use, mass-produceable, low-sample-consumption, easy-to-automate, miniaturized polymer injectors with easy-to-handle solution-exchange membranes makes this system particularly amenable to screening applications.
authors.affiliation: Electrochemistry Laboratory, Swiss Federal Institute of Technology, CH-1015 Lausanne, Switzerland.
authors.forename: Niels
authors.initials: N
authors.lastname: Lion
authors.forename: Véronique
authors.initials: V
authors.lastname: Gobry
authors.forename: Henrik
authors.initials: H
authors.lastname: Jensen
authors.forename: Joël S
authors.initials: JS
authors.lastname: Rossier
authors.forename: Hubert
authors.initials: H
authors.lastname: Girault
mesh_terms: D000804:Angiotensin II / Q000302:isolation & purification; D004209:Disposable Equipment; D006801:Humans; D007328:Insulin / Q000302:isolation & purification; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008567:Membranes, Artificial; D008904:Miniaturization; D009211:Myoglobin / Q000302:isolation & purification; D036103:Nanotechnology; D011433:Propranolol / Q000032:analysis
chemical_list: D007328:Insulin; D008567:Membranes, Artificial; D009211:Myoglobin; D000804:Angiotensin II; D011433:Propranolol