[Changes and its measurement of intracellular Ca2+ during exposure to hyperbaric oxygen].
Objective. To investigate the rule of change of intracellular Ca2+ and its measurement method during exposure to hyperbaric oxygen (HBO). Method. Microfluorescence method was used to measure the changes of intracellular Ca2+ concentration in co-cultured endothelial cells (EC) and smooth muscle cells (SMC) in a specially designed miniature oxygen chamber. Result. (1) [Ca2+]i changes during HBO exposure can be clearly observed directly in the chamber. (2) Within 5 min during 0.2 MPa and 0.3 MPa HBO exposure, EC[Ca2+]i and SMC[Ca2+]i showed a tendency, to increase at first and then decrease with time throughout the exposures, especially the SMC[Ca2+]i. The EC ruptured under exposure to 0.4 MPa HBO. Conclusion. During HBO exposure, EC[Ca2+]i and SMC[Ca2+]i showed biphasic changes, which increased at first and then decreased. The technique and method used were reliable and suitable for investigating of intracellular [Ca2+]i changes under HBO.
Liu
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Naval Medical Research Institute, Shanghai, China.
Liu
J C
JC
Lin
S L
SL
Li
B
B
D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000502:physiology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D004727:Endothelium / Q000166:cytology* / Q000378:metabolism; D006931:Hyperbaric Oxygenation; D008856:Microscopy, Fluorescence; D009130:Muscle, Smooth / Q000166:cytology* / Q000378:metabolism
D002118:Calcium