Gene expression analysis using oligonucleotide arrays produced by maskless photolithography.
Microarrays containing 195,000 in situ synthesized oligonucleotide features have been created using a benchtop, maskless photolithographic instrument. This instrument, the Maskless Array Synthesizer (MAS), uses a digital light processor (DLP) developed by Texas Instruments. The DLP creates the patterns of UV light used in the light-directed synthesis of oligonucleotides. This digital mask eliminates the need for expensive and time-consuming chromium masks. In this report, we describe experiments in which we tested this maskless technology for DNA synthesis on glass surfaces. Parameters examined included deprotection rates, repetitive yields, and oligonucleotide length. Custom gene expression arrays were manufactured and hybridized to Drosophila melanogaster and mouse samples. Quantitative PCR was used to validate the gene expression data from the mouse arrays.
Nuwaysir
Emile F
EF
NimbleGen Systems, Inc., Madison, Wisconsin 53711, USA.
Huang
Wei
W
Albert
Thomas J
TJ
Singh
Jaz
J
Nuwaysir
Kate
K
Pitas
Alan
A
Richmond
Todd
T
Gorski
Tom
T
Berg
James P
JP
Ballin
Jeff
J
McCormick
Mark
M
Norton
Jason
J
Pollock
Tim
T
Sumwalt
Terry
T
Butcher
Lawrence
L
Porter
DeAnn
D
Molla
Michael
M
Hall
Christine
C
Blattner
Fred
F
Sussman
Michael R
MR
Wallace
Rodney L
RL
Cerrina
Franco
F
Green
Roland D
RD
D000818:Animals; D004247:DNA / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D005796:Genes / Q000235:genetics; D017344:Genes, Insect / Q000235:genetics; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry; D010777:Photochemistry / Q000295:instrumentation / Q000379:methods
D015345:Oligonucleotide Probes; D004247:DNA