Identification by PCR of Fusarium culmorum strains producing large and small amounts of deoxynivalenol.
Thirty deoxynivalenol-producing F. culmorum strains, isolated from wheat grains, were incubated in vitro and analyzed for trichothecene production. Seventeen strains produced more than 1 ppm of deoxynivalenol and acetyldeoxynivalenol and were considered high-deoxynivalenol-producing strains, whereas 13 F. culmorum strains produced less than 0.07 ppm of trichothecenes and were considered low-deoxynivalenol-producing strains. For all strains, a 550-base portion of the trichodiene synthase gene (tri5) was amplified and sequenced. According to the tri5 data, the F. culmorum strains tested clustered into two groups that correlated with in vitro deoxynivalenol production. For three high-producing and three low-producing F. culmorum strains, the tri5-tri6 intergenic region was then sequenced, which confirmed the two separate clusters within the F. culmorum strains. According to the tri5-tri6 sequence data, specific PCR primers were designed to allow differentiation of high-producing from low-producing F. culmorum strains.
Bakan
B
B
Laboratoire de Microbiologie et Technologie Céréalières, Institut National de la Recherche Agronomique, 44316 Nantes, France. bakan@nantes.inra.fr
Giraud-Delville
C
C
Pinson
L
L
Richard-Molard
D
D
Fournier
E
E
Brygoo
Y
Y
D001483:Base Sequence; D004271:DNA, Fungal / Q000032:analysis*; D021901:DNA, Intergenic / Q000032:analysis; D005656:Fungal Proteins; D005670:Fusarium / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005784:Gene Amplification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics; D014255:Trichothecenes / Q000096:biosynthesis*
D004271:DNA, Fungal; D021901:DNA, Intergenic; D005656:Fungal Proteins; D014157:Transcription Factors; C094327:Tri6 protein, Fusarium sporotrichioides; D014255:Trichothecenes; C007262:deoxynivalenol