title: NTPDase1 and NTPDase2 immunolocalization in mouse cochlea: implications for regulation of p2 receptor signaling.
abstract: Cellular, molecular, and physiological studies have demonstrated an important signaling role for ATP and related nucleotides acting via P2 receptors in the cochlea of the inner ear. Signal modulation is facilitated by ectonucleotidases, a heterologous family of surface-located enzymes involved in extracellular nucleotide hydrolysis. Our previous studies have implicated CD39/NTPDase1 and CD39L1/NTPDase2, members of the ectonucleoside triphosphate diphosphohydrolase (E-NTPDase) family, as major ATP-hydrolyzing enzymes in the tissues lining the cochlear endolymphatic and perilymphatic compartments. NTPDase1 hydrolyzes both nucleoside triphosphates and diphosphates. In contrast, NTPDase2 is a preferential nucleoside triphosphatase. This study characterizes expression of these E-NTPDases in the mouse cochlea by immunohistochemistry. NTPDase1 can be immunolocalized to the cochlear vasculature and neural tissues (primary auditory neurons in the spiral ganglion). In contrast, NTPDase2 immunolabeling was principally localized to synaptic regions of the sensory inner and outer hair cells, stereocilia and cuticular plates of the outer hair cells, supporting cells of the organ of Corti (Deiters' cells and inner border cells), efferent nerve fibers located in the intraganglionic spiral bundle, and in the outer sulcus and root region of the spiral ligament. This differential expression of NTPDase1 and 2 in the cochlea suggests spatial regulation of P2 receptor signaling, potentially involving different nucleotide species and hydrolysis kinetics.
authors.affiliation: Discipline of Audiology and Department of Physiology, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand. s.vlajkovic@auckland.ac.nz
authors.forename: Srdjan M
authors.initials: SM
authors.lastname: Vlajkovic
authors.forename: Peter R
authors.initials: PR
authors.lastname: Thorne
authors.forename: Jean
authors.initials: J
authors.lastname: Sévigny
authors.forename: Simon C
authors.initials: SC
authors.lastname: Robson
authors.forename: Gary D
authors.initials: GD
authors.lastname: Housley
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D015703:Antigens, CD; D001081:Apyrase / Q000378:metabolism*; D003051:Cochlea / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D051379:Mice; D018613:Microscopy, Confocal; D018048:Receptors, Purinergic P2 / Q000502:physiology
chemical_list: D015703:Antigens, CD; D018048:Receptors, Purinergic P2; D000251:Adenosine Triphosphatases; C027201:ectoATPase; D001081:Apyrase; C067869:CD39 antigen