title: Primary cultures from the marine sponge Xestospongia muta (Petrosiidae, Haplosclerida).
abstract: In the context of the investigations on the origin and in vitro production of bioactive compounds, primary cultures were developed from ectosomal and choanosomal cell suspensions from the sponge Xestospongia muta. Dissociated cells aggregated and reorganized into a striking reticulated network of cells, typical for X. muta. Moreover, in some cultures an isotropic reticulation of small spicules, very similar to that found in the ectosome of adult sponges, was observed. Phytohaemagglutinin promoted aggregation and the reorganization of the cells. HPLC analyses revealed that straight-chain acetylenic compounds were recovered from short-term cultures and that they were synthesized during culture. Heterotrophic bacteria were assumed to be involved in the process. Together our results established that X. muta would be an excellent experimental model to study, in laboratory conditions, the differentiation of the skeleton and the in vitro biosynthesis of straight-chain acetylenic compounds.
authors.affiliation: Laboratory of Cellular Physiology, IBMM, CP 300, Université Libre de Bruxelles, 50 av. F.D. Roosevelt, B-1050 Brussels, Belgium. emaurer@ulb.ac.be
authors.forename: Evelyn
authors.initials: E
authors.lastname: Richelle-Maurer
authors.forename: Raquel
authors.initials: R
authors.lastname: Gomez
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Braekman
authors.forename: Gisèle
authors.initials: G
authors.lastname: Van de Vyver
authors.forename: Rob W M
authors.initials: RW
authors.lastname: Van Soest
authors.forename: Christine
authors.initials: C
authors.lastname: Devijver
mesh_terms: D000480:Alkynes; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017691:Caribbean Region; D002449:Cell Aggregation / Q000187:drug effects / Q000502:physiology; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002457:Cell Extracts / Q000737:chemistry; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D046508:Culture Techniques / Q000379:methods*; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000737:chemistry; D005431:Florida; D008386:Marine Biology / Q000379:methods; D009024:Morphogenesis / Q000187:drug effects / Q000502:physiology; D011161:Porifera / Q000737:chemistry / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism; D011786:Quality Control; D012623:Seawater; D013696:Temperature
chemical_list: D000480:Alkynes; D000900:Anti-Bacterial Agents; D002457:Cell Extracts; D005231:Fatty Acids, Unsaturated; C031203:2-hexadecynoic acid