title: Dynamics of vitellogenin mRNA expression and changes in hemolymph vitellogenin levels during ovarian maturation in the giant freshwater prawn Macrobrachium rosenbergii.
abstract: The dynamics of vitellogenin mRNA expression during ovarian maturation in Macrobrachium rosenbergii were examined by measuring hemolymph vitellogenin (Vg) levels and Vg mRNA expression in the hepatopancreas and ovary at differing reproductive stages in both intact and eyestalk ablated animals. Vg mRNA was quantified using real-time RT-PCR and hemolymph Vg was measured by enzyme immunoassay. In intact animals, Vg mRNA levels in the hepatopancreas and hemolymph Vg levels showed a gradual increase during the molt cycle concomitant with increasing gonadosomatic index (GSI), with Vg levels decreasing prior to ecdysis although GSI continued to increase. Eyestalk ablation was seen to accelerate Vg synthesis as well as ovarian maturation, although it did not alter the overall pattern of Vg expression. Vg mRNA expression was negligible in the ovary of both intact and eyestalk ablated animals, confirming that the hepatopancreas is the principal site of Vg synthesis in M. rosenbergii with the ovary being only a minor contributor. This study has shown that Vg synthesis is correlated to ovarian maturation and the molt cycle in M. rosenbergii.
authors.affiliation: Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305-8686, Japan.
authors.forename: Vidya
authors.initials: V
authors.lastname: Jayasankar
authors.forename: Naoaki
authors.initials: N
authors.lastname: Tsutsui
authors.forename: Safiah
authors.initials: S
authors.lastname: Jasmani
authors.forename: Hisako
authors.initials: H
authors.lastname: Saido-Sakanaka
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Yang
authors.forename: Atsuro
authors.initials: A
authors.lastname: Okuno
authors.forename: Thi Thanh Hien
authors.initials: TT
authors.lastname: Tran
authors.forename: Katsumi
authors.initials: K
authors.lastname: Aida
authors.forename: Marcy N
authors.initials: MN
authors.lastname: Wilder
mesh_terms: D000818:Animals; D033364:Decapoda / Q000235:genetics* / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006458:Hemolymph / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D010053:Ovary / Q000254:growth & development*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014819:Vitellogenins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D014819:Vitellogenins