title: Controlling the lateral distribution and alignment of human gingival fibroblasts by micropatterned polysaccharide surfaces.
abstract: Adhesion and alignment of primary human gingival fibroblast to Petri dishes were controlled by proper modification of the surface chemistry of the solid substrate. In particular, air plasma (glow-discharge) treatment, coupled to a masking technique, was used to pattern cell-adhesive areas on a cell-adhesion-resistant alginate coated surface. Fibroblasts were successfully confined to the air plasma-treated areas, which show a much greater growth rate and cell density as compared to the original alginate coated surface. Beside the implications for the study of the mechanisms of bio-adhesion at hydrophilic surfaces, these systems can be of interest for a number of applications in dental surgery and materials.
authors.affiliation: Nobil Bio Ricerche, Villafranca d'Asti, Italy.
authors.forename: Clara
authors.initials: C
authors.lastname: Cassinelli
authors.forename: Marco
authors.initials: M
authors.lastname: Morra
authors.forename: Angelo
authors.initials: A
authors.lastname: Carpi
authors.forename: Roberto
authors.initials: R
authors.lastname: Giardino
authors.forename: Milena
authors.initials: M
authors.lastname: Fini
mesh_terms: D000464:Alginates / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects*; D005881:Gingiva / Q000166:cytology; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D011134:Polysaccharides / Q000494:pharmacology*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D011134:Polysaccharides; D020723:Glucuronic Acid