title: Boron supplementation and activated factor VII in healthy men.
abstract: OBJECTIVE
  The aim of the present study was to determine whether postprandial concentrations of the active component of serine protease coagulation factor VII (VIIa) were lowered by acute boron supplementation in vivo.
  
  
  METHODS
  An acute, randomized, placebo-controlled, double blind, cross-over study.
  
  
  METHODS
  Free-living population.
  
  
  METHODS
  Fifteen apparently healthy men, aged 45-65 y.
  
  
  METHODS
  Subjects visited the centre on two occasions, with the study days separated by a minimum of 2 weeks. Following collection of a fasting blood sample, subjects received either placebo or acute bolus of 11.6 mg boron (given as 102.6 mg sodium tetraborate decahydrate) together with a standard fat-rich meal. Blood samples were obtained at 1, 2, 4 and 6 h after the administration of the test meal, during which time subjects were at liberty to consume deionized water only. Blood samples were assayed for concentrations of insulin, glucose, lipids and boron. Measurement of the concentration of activated factor VIIa and of factor VII antigen, and of the activity of coagulation factors VII, IX and X was also carried out.
  
  
  RESULTS
  Plasma boron concentrations were significantly higher following consumption of the boron supplement compared with placebo (0.124+/-0.02 vs 0.008+/-0.01 mg/l; P< or =0.001). There was no significant effect of acute boron supplementation on plasma insulin and glucose concentration or on blood lipid or coagulation factor profile. Factor VIIa rose significantly following consumption of the high fat meal (1.05+/-0.07 vs 1.26+/-0.07; P< or =0.001), but this increase was not altered by boron supplementation.
  
  
  CONCLUSIONS
  Results from this study suggest that acute boron supplementation (at 11.6 mg boron) does not alter the activity of factor VIIa following consumption of a high-fat meal.
  
  
  BACKGROUND
  This work was funded by Borax Europe Ltd.
authors.affiliation: Northern Ireland Centre for Diet and Health, University of Ulster, Coleraine, Northern Ireland, UK. j.wallace@ulster.ac.uk
authors.forename: J M W
authors.initials: JM
authors.lastname: Wallace
authors.forename: M P A
authors.initials: MP
authors.lastname: Hannon-Fletcher
authors.forename: P J
authors.initials: PJ
authors.lastname: Robson
authors.forename: W S
authors.initials: WS
authors.lastname: Gilmore
authors.forename: S A
authors.initials: SA
authors.lastname: Hubbard
authors.forename: J J
authors.initials: JJ
authors.lastname: Strain
mesh_terms: D000368:Aged; D001786:Blood Glucose / Q000032:analysis; D001895:Boron / Q000008:administration & dosage / Q000097:blood* / Q000494:pharmacology*; D018592:Cross-Over Studies; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism*; D019587:Dietary Supplements; D015942:Factor VIIa / Q000187:drug effects / Q000378:metabolism*; D005215:Fasting; D006801:Humans; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D019518:Postprandial Period
chemical_list: D001786:Blood Glucose; D004041:Dietary Fats; D007328:Insulin; D008055:Lipids; D015942:Factor VIIa; D001895:Boron