title: Activins A, AB, and B inhibit hepatocyte growth factor synthesis by MRC-5 human lung fibroblasts.
abstract: The effect of activins A, AB, and B on hepatocyte growth factor (HGF) synthesis stimulated by 12-O-tetradecanoylphorbol beta-acetate (TPA) was studied in MRC-5 human lung fibroblasts. Activins A, AB, and B inhibited the increase in HGF secretion induced by TPA in different dose-dependent manners and potencies. At 5 ng/ml, activins A and AB inhibited the increase approximately 30% and 10%, respectively, and at 25 ng/ml both activins produced almost maximal inhibition, i.e., approximately 40%. Activin B caused 10% inhibition at 12 ng/ml, and at 25 ng/ml produced almost maximal inhibition, approximately 30%. Further analysis with activin A indicated that the inhibition was caused by decreased HGF mRNA levels, followed by decreased cellular HGF levels. At 25 ng/ml, activin A inhibited the increase in HGF in the cellular lysate and the increase in HGF mRNA level approximately 80% and 40%, respectively.
authors.affiliation: Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, Tokyo, Japan. niimi@nihs.go.jp
authors.forename: Shingo
authors.initials: S
authors.lastname: Niimi
authors.forename: Masashi
authors.initials: M
authors.lastname: Hyuga
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kazama
authors.forename: Masami
authors.initials: M
authors.lastname: Inagawa
authors.forename: Taiichiro
authors.initials: T
authors.lastname: Seki
authors.forename: Toyohiko
authors.initials: T
authors.lastname: Ariga
authors.forename: Tetsu
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Takao
authors.initials: T
authors.lastname: Hayakawa
mesh_terms: D028341:Activins / Q000494:pharmacology* / Q000502:physiology; D000818:Animals; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D006801:Humans; D028322:Inhibin-beta Subunits / Q000494:pharmacology* / Q000502:physiology; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013552:Swine
chemical_list: C422405:activin A; C044147:activin B; C103008:inhibin beta A subunit; D028341:Activins; D017228:Hepatocyte Growth Factor; D028322:Inhibin-beta Subunits