title: Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
abstract: Dendritic cells (DCs) are professional antigen-presenting cells with a highly immunostimulatory function and the capacity to activate naïve T cells. In recent years the rapid progress in mouse and human DC research can be mainly attributed to the generation of DCs from precursor cells in vitro, although a lack of reagents has hampered DC research in many large animal models. Here we describe the generation and characterization of ovine monocyte-derived DCs in vitro. In addition to the characteristic morphology and non-adherence of DCs, peripheral blood mononuclear cell monocytes cultured with ovine granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) expressed CD11c and major histocompatibility complex (MHC) class II, but did not express CD14. High levels of endocytosis and an ability to stimulate antigen-specific proliferation of CD4 T lymphocytes were also demonstrated.
authors.affiliation: Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge, UK.
authors.forename: Simon S M
authors.initials: SS
authors.lastname: Chan
authors.forename: Ian
authors.initials: I
authors.lastname: McConnell
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Blacklaws
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D004705:Endocytosis / Q000276:immunology; D005260:Female; D016130:Immunophenotyping; D015847:Interleukin-4 / Q000096:biosynthesis; D008297:Male; D009000:Monocytes / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis; D012756:Sheep / Q000276:immunology*
chemical_list: D011994:Recombinant Proteins; D015847:Interleukin-4