Overexpressed yeast mitochondrial putative RNA helicase Mss116 partially restores proper mtRNA metabolism in strains lacking the Suv3 mtRNA helicase.
RNA helicase, encoded by the Saccharomyces cerevisiae nuclear gene SUV3, is a subunit of the mitochondrial (mt) degradosome: an enzyme complex that takes part in turnover of mtRNAs. Deletion of the SUV3 gene leads to a variety of disturbances in mtRNA metabolism and results in respiratory incompetence of yeast cells. Here we show that the nuclear gene MSS116, which codes for a mitochondrial putative RNA helicase necessary for splicing of several mt introns, can suppress the lack of the SUV3 gene. Overexpression of the Mss116 putative helicase from a multicopy plasmid present in the SUV3-deleted strains partially restores respiratory competence, brings the steady-state levels of COB and ATP6/8 mRNA back almost to normal and lowers the accumulation of 21S rRNA and ATP6/8 RNA precursors to the wild-type levels. To the best of our knowledge, this is the first reported case of a substitution of one RNA helicase by another, belonging to a different class of RNA helicases.
Minczuk
Michal
M
Department of Genetics, University of Warsaw and Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106 Warsaw, Poland.
Dmochowska
Aleksandra
A
Palczewska
Malgorzata
M
Stepien
Piotr P
PP
D053487:DEAD-box RNA Helicases; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008928:Mitochondria / Q000201:enzymology*; D012313:RNA / Q000378:metabolism*; D020365:RNA Helicases / Q000235:genetics* / Q000378:metabolism*; D012331:RNA, Fungal / Q000378:metabolism; D000077278:RNA, Mitochondrial; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins
D012331:RNA, Fungal; D000077278:RNA, Mitochondrial; D029701:Saccharomyces cerevisiae Proteins; D012313:RNA; C058150:MSS116 protein, S cerevisiae; C075918:SUV3 protein, S cerevisiae; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases