title: Ischaemia-reperfusion injury of rat ovary and the effects of vitamin C, mannitol and verapamil.
abstract: BACKGROUND
  In this prospective controlled study, the aim was to examine the effects of vitamin C, mannitol and verapamil on adnexial ischaemia-reperfusion injury in the rat ovary.
  
  
  METHODS
  Thirty-six female Wistar rats were used. In the controls (group 1), only laparotomy was performed. In group 2, ovarian ischaemia was produced and the bilateral ovaries were surgically removed 4 h later. In group 3, an ischaemic period of 4 h was followed by reperfusion for 1 h; the bilateral ovaries were then removed. In groups 4, 5 and 6, after 4 h of ischaemia, either vitamin C, mannitol or verapamil respectively was infused before reperfusion; after 1 h of reperfusion the ovaries were removed. Thiobarbituric acid reactive substance (TBARS) levels were measured in all ovary tissues.
  
  
  RESULTS
  TBARS levels of the reperfusion group were significantly higher than those of groups treated with vitamin C or mannitol (P = 0.013 and P = 0.045 respectively), but not of the verapamil group.
  
  
  CONCLUSIONS
  Vitamin C and mannitol were found to be effective in reducing ischaemia-reperfusion injury of the ovary during its early stages, but verapamil was ineffective.
authors.affiliation: Kirikkale University Faculty of Medicine, Ankara, Turkey. nsagsoz@isbank.net.tr
authors.forename: Nevin
authors.initials: N
authors.lastname: Sağsöz
authors.forename: Uçler
authors.initials: U
authors.lastname: Kisa
authors.forename: Alparslan
authors.initials: A
authors.lastname: Apan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000627:therapeutic use*; D002121:Calcium Channel Blockers / Q000627:therapeutic use*; D005260:Female; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D007511:Ischemia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008353:Mannitol / Q000627:therapeutic use*; D010053:Ovary / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014700:Verapamil / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D002121:Calcium Channel Blockers; D016166:Free Radical Scavengers; D017392:Thiobarbituric Acid Reactive Substances; D008353:Mannitol; D014700:Verapamil; D001205:Ascorbic Acid