title: A first-generation EST RH comparative map of the porcine and human genome.
abstract: We have constructed a first-generation EST radiation hybrid comparative map of the porcine genome by assigning 1,058 markers to the IMpRH(7000) panel. Chromosomal localization was determined with a 2pt LOD of 4.8 for 984 markers, using the IMpRH mapping tool. Annotated ESTs represent 46.2% or 489 of the markers. Marker distribution was not stochastic and ranged from 0.41 for SSC8 to 1.77 for SSC12, respectively. Two hundred fifty-one markers assigned to the physical map of the pig did not find a homologous sequence in V22 of the human genome assembly, indicative of gaps in the assembled human genome sequence. The comparative porcine/human map covers 3,290 MB, or 98.3% of the presumed size of the human genome. However, 60 breakpoints were identified between chromosomes, as well as 90 micro-rearrangements within synteny groups. Six porcine chromosomes-SSC2, 5, 6, 7, 12, and 14-correspond to the three gene-richest human chromosomes, HSA17, 19, and 22, and show above average marker density. Porcine Chrs 1, 8, 11, and X display a low DNA/marker ratio and correspond to the 'genome deserts' on HSA 18, 4, 13, and X.
authors.affiliation: Department of Animal Biotechnology, College of Agriculture, Biotechnology and Natural Resources, University of Nevada, Reno, Nevada 89557, USA. arink@cabnr.unr.edu
authors.forename: Anette
authors.initials: A
authors.lastname: Rink
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Santschi
authors.forename: Katie M
authors.initials: KM
authors.lastname: Eyer
authors.forename: Benjamin
authors.initials: B
authors.lastname: Roelofs
authors.forename: Markus
authors.initials: M
authors.lastname: Hess
authors.forename: Myra
authors.initials: M
authors.lastname: Godfrey
authors.forename: Elif K
authors.initials: EK
authors.lastname: Karajusuf
authors.forename: Martine
authors.initials: M
authors.lastname: Yerle
authors.forename: Denis
authors.initials: D
authors.lastname: Milan
authors.forename: Craig W
authors.initials: CW
authors.lastname: Beattie
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D006822:Hybrid Cells; D021101:Radiation Hybrid Mapping / Q000379:methods / Q000662:veterinary*; D013552:Swine / Q000235:genetics*; D026801:Synteny
chemical_list: D005819:Genetic Markers