title: Pseudothrombocytopenia: a report of a new method to count platelets in a patient with EDTA- and temperature-independent antibodies of the IgM type.
abstract: Pseudothrombocytopenia is usually associated with blood specimens anticoagulated with ethylenediamine tetraacetic acid (EDTA) or other anticoagulants. It may be caused by temperature-independent, EDTA-dependent antibodies of the immunoglobulin-M (IgM) type. Here a patient with EDTA-independent and temperature-independent pseudothrombocytopenia mediated by IgM or IgM-containing immune complex is reported, and a reliable method is described for a proper counting of platelets in such cases.
authors.affiliation: Department of Clinical Chemistry, University Medical Center St Radboud, PO Box 9101, 6500 HB Nijmegen, The Netherlands. w.vandermeer@ckcl.azn.nl
authors.forename: W
authors.initials: W
authors.lastname: van der Meer
authors.forename: W
authors.initials: W
authors.lastname: Allebes
authors.forename: A
authors.initials: A
authors.lastname: Simon
authors.forename: Y
authors.initials: Y
authors.lastname: van Berkel
authors.forename: M H
authors.initials: MH
authors.lastname: de Keijzer
mesh_terms: D000918:Antibody Specificity / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology / Q000378:metabolism; D004492:Edetic Acid; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010976:Platelet Count / Q000379:methods*; D013696:Temperature; D013921:Thrombocytopenia / Q000097:blood* / Q000473:pathology
chemical_list: D007075:Immunoglobulin M; D004492:Edetic Acid