title: Effect of human serum albumin on cell attachment of human gingival epithelial cells.
abstract: OBJECTIVE
  To investigate the effect of human serum albumin (HSA) on cell attachment of human gingival epithelial cells (HGE).
  
  
  METHODS
  HGE were primary cultured with keratinocyte serum-free medium (KSFM) and dispase. The cultured cells were immunohistochemically stained by monoclonal anti-pan cytokeratin. MTT test was employed to investigate the influence of HSA on the cell attachment on polystyrene surface. The cell growth curve of HGE which were cultured in KSFM with 50 g/L HSA was observed.
  
  
  RESULTS
  The results showed significant decrease in cell numbers within 8 hours after HGE were inoculated, in which the polystyrene surface was preincubated with 50 g/L HSA. But it did not prove to be the case from 10 hours to 24 hours after HGE were inoculated. There were no significant difference within 24 hours in cell numbers between cultured in KSFM with 50 g/L HSA and control. The cell numbers in cell growth curve of HGE in KSFM with and without 50 g/L HSA did not show significant difference.
  
  
  CONCLUSIONS
  HSA preincubation on polystyrene were produce inhibitory effect of HGE attachment in early stage.
authors.affiliation: Department of Stomatology, General Navy Hospital, Beijing 100037, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Shenggen
authors.initials: S
authors.lastname: Shi
authors.forename: Yingliang
authors.initials: Y
authors.lastname: Song
authors.forename: Songshan
authors.initials: S
authors.lastname: Lin
authors.forename: Hang
authors.initials: H
authors.lastname: Guo
authors.forename: He
authors.initials: H
authors.lastname: Zhang
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D011137:Polystyrenes; D012709:Serum Albumin / Q000494:pharmacology*
chemical_list: D011137:Polystyrenes; D012709:Serum Albumin