rDNA (18S-28S and 5S) colocalization and linkage between ribosomal genes and (TTAGGG)(n) telomeric sequence in the earthworm, Octodrilus complanatus (Annelida: Oligochaeta: Lumbricidae), revealed by single- and double-color FISH.
Spermatogonial and metaphase I chromosomes of the lumbricid earthworm Octodrilus complanatus (Annelida: Oligochaeta) were examined using fluorescent in situ hybridization (FISH) with three repetitive DNA probes-5S rDNA, 18S-26S rDNA, and (TTAGGG)(n). Single-color FISH consistently mapped one chromosome pair per spread using either 5S rDNA or 18S-26S rDNA as probes. Simultaneous (18S-26S)-5S and (18S-26S)-(TTAGGG)(n) FISH demonstrated that repeated units of the two ribosomal families were overlapped and closely associated with telomeric sequences.
Vitturi
R
R
Dipartimento di Biologia Animale, Università di Palermo, Palermo, Italy. zuvitcol@unipa.it
Colomba
M S
MS
Pirrone
A M
AM
Mandrioli
M
M
D000818:Animals; D004275:DNA, Ribosomal; D008040:Genetic Linkage; D017404:In Situ Hybridization, Fluorescence; D009835:Oligochaeta / Q000235:genetics*; D016615:Telomere
D004275:DNA, Ribosomal