title: B lymphocyte memory: role of stromal cell complement and FcgammaRIIB receptors.
abstract: To dissect the influence of CD21/CD35 and FcgammaRIIB in antigen retention and humoral memory, we used an adoptive transfer model in which antigen-primed B and T lymphocytes were given to sublethally irradiated wild-type mice or mice deficient in CD21/CD35 (Cr2(-/-)) or FcgammaRIIB receptors (FcgammaRIIB(-/-)). Cr2(-/-) chimeras showed impaired memory as characterized by a decrease in antibody titer, reduced frequency of antibody secreting cells, an absence of affinity maturation, and significantly reduced recall response. The impaired memory in Cr2(-/-) chimeras corresponded with the reduced frequency of antigen-specific memory B cells. Interestingly, FcgammaRIIB(-/-) chimeras showed a differential phenotype with impaired splenic but normal bone marrow responses. These data suggest that CD21/CD35 on stroma, including follicular dendritic cells, is critical to the maintenance of long-term B lymphocyte memory.
authors.affiliation: Center for Blood Research and Department of Pathology, Harvard University, Boston, MA 02115, USA.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Barrington
authors.forename: Olga
authors.initials: O
authors.lastname: Pozdnyakova
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Zafari
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Benjamin
authors.forename: Michael C
authors.initials: MC
authors.lastname: Carroll
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D015703:Antigens, CD / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D006241:Haptens; D006433:Hemocyanins / Q000276:immunology; D007156:Immunologic Memory / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D017463:Receptors, Complement 3b / Q000276:immunology*; D017464:Receptors, Complement 3d / Q000276:immunology*; D017452:Receptors, IgG / Q000276:immunology*; D017154:Stromal Cells / Q000276:immunology*; D013997:Time Factors
chemical_list: C098951:4-hydroxy-3-nitrophenylacetyl-keyhole limpet hemocyanin; D000906:Antibodies; D015703:Antigens, CD; C080571:Fc gamma receptor IIB; D006241:Haptens; D017463:Receptors, Complement 3b; D017464:Receptors, Complement 3d; D017452:Receptors, IgG; D006433:Hemocyanins