title: Both B and T lymphocytes may be clonally involved in myelofibrosis with myeloid metaplasia.
abstract: A combination of magnetic cell sorting (MACS) and fluorescent in situ hybridization (FISH) techniques was used to detect clonal cytogenetic markers in different myeloid and lymphoid cell types of the peripheral blood from 4 patients with myelofibrosis with myeloid metaplasia (MMM) that was associated with either a 13q- or a 20q- karyotypic abnormality. Interphase cytogenetics studies demonstrated abnormal clonal FISH signal patterns in neutrophil, myeloid, erythroid, megakaryocyte, and B- and T-cell preparations in 3 of the 4 patients. In one patient, FISH results were within normal limits in T cells and slightly abnormal in B cells. In general, the percentage of abnormal nuclei was variable in both lymphocyte populations but always higher in B lymphocytes compared with T lymphocytes. The current study provides direct evidence for the clonal involvement of both B and T lymphocytes in MMM. A larger study is needed to clarify the relevance of the observed interpatient heterogeneity in clonal constitution.
authors.affiliation: Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Terra L
authors.initials: TL
authors.lastname: Reeder
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Bailey
authors.forename: Gordon W
authors.initials: GW
authors.lastname: Dewald
authors.forename: Ayalew
authors.initials: A
authors.lastname: Tefferi
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D001402:B-Lymphocytes / Q000648:ultrastructure*; D019070:Cell Lineage; D002467:Cell Nucleus / Q000648:ultrastructure; D002872:Chromosome Deletion; D002882:Chromosomes, Human, Pair 13; D002890:Chromosomes, Human, Pair 20; D002999:Clone Cells / Q000648:ultrastructure; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D007621:Karyotyping; D008297:Male; D008533:Megakaryocytes / Q000648:ultrastructure; D022423:Myeloid Cells / Q000648:ultrastructure; D009504:Neutrophils / Q000648:ultrastructure; D009928:Organ Specificity; D055728:Primary Myelofibrosis / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D013601:T-Lymphocytes / Q000648:ultrastructure*
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm