Upregulation of Ku expression in human neuroectodermal tumor cells after retroviral DNA integration.
OBJECTIVE
To examine the expression of DNA-dependent protein kinase (DNA-PK); including DNA-PKcs, Ku70 and Ku80 in human neuroectodermal tumor cells with or without retroviral DNA integration.


METHODS
RT-PCR assay was used to examine the transcript of DNA-PK. Western blot and immunocytochemistry assays were used to examine the protein level of DNA-PK.


RESULTS
The expression of DNA-PKcs was similar in the cells with or without retroviral DNA integration, but the expression of Ku (both mRNA and protein for Ku70 and only protein for Ku80) was higher in cells with retroviral DNA integration than in cells without retroviral DNA integration.


CONCLUSIONS
The expression of Ku but not the expression of DNA-PKcs is induced by retroviral DNA integration. Ku70 may play an important role in the regulation of Ku function in response to retroviral DNA integration.
Liu
Xuan
X
Laboratory of Genomic Physiology, School of Life Sciences, Fudan University, Shanghai, PR China.
Lin
Junji
J
Huang
Jia
J
Li
Lishu
L
Liu
Lei
L
Xu
Ping
P
D034961:Antigens, Nuclear; D004265:DNA Helicases; D051747:DNA-Activated Protein Kinase; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D000072200:Ku Autoantigen; D017599:Neuroectodermal Tumors / Q000378:metabolism / Q000821:virology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D012190:Retroviridae / Q000472:pathogenicity / Q000502:physiology*; D014407:Tumor Cells, Cultured; D016662:Virus Integration
D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human; D017346:Protein Serine-Threonine Kinases; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen