WT1 downregulation during K562 cell differentiation and apoptosis induced by bufalin.
OBJECTIVE
To study the change of WT1 gene expression during human leukemic K562 cell differentiation and apoptosis induced by bufalin.


METHODS
Cell viability was determined by trypan blue exclusion, cell differentiation and apoptosis by nitro blue tetrazolium (NBT) reduction test, morphology and flow cytometry, expression of WT1 protein by Western blot analysis, and expression of WT1 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR).


RESULTS
(1) The cell proliferation was inhibited by bufalin and the IC(50) at 24, 48, 72 h were 0.026, 0.032 and 0.006 micro mol/L, respectively. (2) Bufalin induced K562 cell differentiation towards macrophage/monocyte within concentration from 0.01 to 0.05 micro mol/L and apoptosis at higher than 0.026 micro mol/L. (3) The expression of WT1 protein and mRNA were downregulated by bufalin in the initial stage of differentiation and apoptosis induced by bufalin.


CONCLUSIONS
K562 cell differentiation and apoptosis induced by bufalin might relate to the downregulation of WT1 expression.
Liu
Yunpeng
Y
Department of Medical Oncology, The First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Qu
Xiujuan
X
Wang
Pingping
P
Tian
Xin
X
Luo
Ying
Y
Liu
Shizhou
S
Lu
Xianglan
X
D017209:Apoptosis; D002454:Cell Differentiation; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D025721:WT1 Proteins / Q000235:genetics
D025721:WT1 Proteins