A comparison of ecotoxicological tests.
A simple, inexpensive and rapid method of determining toxicity by using a bacterium as the indicator organism was developed and compared with 23 other tests. The average correlation coefficient when comparing these 23 tests with the present test was 0.800, ranging from 0.580 to 0.950. Eleven of the tests were compared in detail by using 35 of the chemicals on the Multicentre Evaluation of In Vitro Cytotoxicity list of test chemicals. Comparing results from the present test with test results for these 35 chemicals with Microtox, Biotox, Daphnia magna, rat hepatocytes and ascites tumour cell resulted in correlation coefficients ranging from 0.871 to 0.933. Comparisons of the test data with rodent LD50 values, human lethal dose estimates from autopsies and human lethal doses obtained from the literature provided correlation coefficients ranging from 0.580 to 0.770, indicating that the test compares less favourably with these methods. This test provides data comparable to data from other ecotoxicological tests.
Botsford
James L
JL
Department of Biology, New Mexico State University, P.O. Box 30001, Las Cruces, NM 88003-8001, USA.
D000826:Animal Testing Alternatives / Q000379:methods*; D000818:Animals; D003621:Daphnia / Q000187:drug effects; D006801:Humans; D007928:Lethal Dose 50; D010084:Oxidation-Reduction; D021281:Quantitative Structure-Activity Relationship; D051381:Rats; D015203:Reproducibility of Results; D016962:Sinorhizobium meliloti / Q000187:drug effects* / Q000378:metabolism; D013778:Tetrazolium Salts / Q000378:metabolism*; D018675:Toxicity Tests / Q000379:methods*
D013778:Tetrazolium Salts