title: Evaluation of acetylcholinesterase activity in several zooplanktonic crustaceans.
abstract: Zooplankton are an essential component of the marine and estuarine food chains. The ecotoxicological risk to zooplanktonic communities, estimated through the use of the biomarkers, can be used as an early warning signal of a potential alteration of the ecosystem health. The aim of this project was to evaluate the potential use of several biomarkers (esterases, mixed function oxidases, porphyrins) in zooplanktonic organisms. The acetylcholinesterase activity (AChE) was determined in homogenates of whole organisms of 10 different zooplanktonic crustaceans. Mean activity of AChE was 10.05 micromol/min/g for Acartia margalefi; 3.30 for Acartia latisetosa; 79.70 for Siriella clausi; 49.97 for Diamysis bahirensis; 7.48 for Siriella armata; 14.20 for Mysidopsis gibbosa; 4.49 for Euphausia crystallorophias; 1.66 for Euphausia superba; 2.74 for Streetsia challengeri; 13.26 for Meganycthiphanes norvegica. The species moreover showed a linear increase in enzyme activity in relation to the increase in sample concentration. The key result of this study concerns the different AChE activity basal values of the crustaceans' different species.
authors.affiliation: Department of Animal Biology and Marine Ecology, University of Messina, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Minutoli
authors.forename: M C
authors.initials: MC
authors.lastname: Fossi
authors.forename: L
authors.initials: L
authors.lastname: Guglielmo
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis / Q000494:pharmacology*; D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D003445:Crustacea / Q000502:physiology*; D004784:Environmental Monitoring; D012016:Reference Values; D015048:Zooplankton / Q000502:physiology*
chemical_list: D015415:Biomarkers; D000110:Acetylcholinesterase