Capillary electrophoresis with solid-state electrochemiluminescence detector.
We report capillary electrophoresis coupling to a solid-state electrochemiluminescence (ECL) detector for the first time. The solid-state ECL detector was fabricated by immobilizing the ECL reagent tris(2,2'-bipyridyl)ruthenium (TBR) in poly-(p-styrenesulfonate)-silica-poly(vinyl alcohol) grafting 4-vinylpyridine copolymer films. The excellent stability of the solid-state ECL detector in the phosphate solution satisfied application in CE. The CE with solid-state ECL detector system was characterized using tripropylamine (TPA) and proline. The influences of detection potential, the concentration of TBR in the film, and pH value of ECL buffer were investigated. The linear range for TPA and proline was 0.005-10 microM and 5-10 mM with correlation coefficients of 0.997 and 0.998, respectively. The detection limit (signal-to-noise ratio S/N = 3) was estimated to be 0.002 and 2.0 microM for TPA and proline, respectively. The relative standard deviations for 1.0 microM TPA and 1.0 mM proline were 8.7% and 7.5% with theoretical plate numbers of 70 000 and 16 000, respectively. Compared with the CE-ECL of TBR in aqueous solution, the CE coupling with solid-state ECL detector system gave the same sensitivity of analysis.
Cao
Weidong
W
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, China.
Jia
Jianbo
J
Yang
Xiurong
X
Dong
Shaojun
S
Wang
Erkang
E
D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000592:standards; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D009942:Organometallic Compounds; D010084:Oxidation-Reduction; D011392:Proline / Q000032:analysis; D011437:Propylamines / Q000032:analysis; D012680:Sensitivity and Specificity
D009942:Organometallic Compounds; D011437:Propylamines; C415768:tris(2,2'-bipyridyl)ruthenium(II); C096226:tripropylamine; D011392:Proline