[Regulation by ovarian hormones of alpha 1,3-fucosyltransferase gene (FUT9) expression in human endometrium].
Recently it was found that alpha1,3-fucosyltransferase (FUT9) was an essential enzyme involved in the synthesis of Le(X) oligosaccharide. In this article, the expression of FUT9 gene at different hormonal levels and the regulating mechanism of Le(X) synthesis in human endometrium were investigated by using RT-PCR Immuno histochemistry staining and Western blotting. FUT9 mRNA was detected with human endometrium, and the levels of mRNA in secretory endometrium and in proliferative endometrium from patients who had taken mifepristone before operation, were higher than tha t of proliferative endometrium (P<0.01). Le(X) antigen was mainly located on the surface of epithelium. The floating level of Le(X) antigen expression was similar to that of FUT9 gene. There were three (33 kD, 35 kD and 85 kD) LeX carrying proteins expressed in human endometrium, mifepristone treatment made 35 kD b and disappear. The results indicate that FUT9 gene is obviously expressed in human endometrium and can be upregulated by progesterone, and ovary hormones regulate the expression of Le(X) at the level of transcription.
Li
Hong
H
Department of Biochemistry, Institute of Glycobiology, Dalian Medical Univ ersity, Dalian 116027, China. zmhu@mail.dlptt.ln.cn
Kong
Ying
Y
Yan
Bin
B
D000284:Administration, Oral; D015153:Blotting, Western; D004717:Endometrium / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D005260:Female; D005647:Fucosyltransferases / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006727:Hormone Antagonists / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D016256:Lewis X Antigen / Q000032:analysis; D015735:Mifepristone / Q000008:administration & dosage / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D006727:Hormone Antagonists; D016256:Lewis X Antigen; D015735:Mifepristone; D005647:Fucosyltransferases; C031692:galactoside 3-fucosyltransferase