Comparison of genetic variability between Czech and foreign isolates of phytopathogenic bacteria Clavibacter michiganensis subsp. sepedonicus by Rep-PCR technique.
Repetitive-sequence-based polymerase chain reaction (Rep-PCR) method was used for analysis of genetic variability among bacterial populations from different world locations. Collection of 26 Czech and 13 foreign strains of Clavibacter michiganensis subsp. sepedonicus was amplified using BOX primer targeting to repetitive motif occurring in eubacterial genomes. Genetic fingerprints were visually compared and statistically evaluated by cluster analysis. Genetic similarity was estimated to be approximately 80% among all tested strains. Populations of these bacteria seem to be highly homogeneous; potential influence of geographic origin was not confirmed.
Fousek
J
J
Department of Plant Virology, Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, 370 05 Ceské Budĕjovice, Czechia. fousek@umbr.cas.cz
Mráz
I
I
Petrzik
K
K
D000192:Actinomycetales / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D018153:Czech Republic; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D014644:Genetic Variation; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012091:Repetitive Sequences, Nucleic Acid; D011198:Solanum tuberosum / Q000382:microbiology*
D004269:DNA, Bacterial