title: Cloning, expression and characterization of a mammalian Nudix hydrolase-like enzyme that cleaves the pyrophosphate bond of UDP-glucose.
abstract: A distinct UDP-glucose (UDPG) pyrophosphatase (UGPPase, EC 3.6.1.45) has been characterized using pig kidney ( Sus scrofa ). This enzyme hydrolyses UDPG, the precursor molecule of numerous glycosylation reactions in animals, to produce glucose 1-phosphate (G1P) and UMP. Sequence analyses of the purified enzyme revealed that, similar to the case of a nucleotide-sugar hydrolase controlling the intracellular levels of ADP-glucose linked to glycogen biosynthesis in Escherichia coli [Moreno-Bruna, Baroja-Fernández, Muñoz, Bastarrica-Berasategui, Zandueta-Criado, Rodri;guez-López, Lasa, Akazawa and Pozueta-Romero (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 8128-8132], UGPPase appears to be a member of the ubiquitously distributed group of nucleotide pyrophosphatases designated Nudix hydrolases. A complete cDNA of the UGPPase-encoding gene, designated UGPP, was isolated from a human thyroid cDNA library and expressed in E. coli. The resulting cells accumulated a protein that showed kinetic properties identical to those of pig UGPPase.
authors.affiliation: JCR Pharmaceuticals Co. Ltd., Pharmacological Science, Research Division, 2-2-10 Murotani, Nishi-ku, Kobe 651-2241, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Yagi
authors.forename: Edurne
authors.initials: E
authors.lastname: Baroja-Fernández
authors.forename: Ryuji
authors.initials: R
authors.lastname: Yamamoto
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: Muñoz
authors.forename: Takashi
authors.initials: T
authors.lastname: Akazawa
authors.forename: Kyoung Su
authors.initials: KS
authors.lastname: Hong
authors.forename: Javier
authors.initials: J
authors.lastname: Pozueta-Romero
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D005943:Gluconeogenesis / Q000502:physiology; D006801:Humans; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D011755:Pyrophosphatases / Q000235:genetics* / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D013379:Substrate Specificity; D034421:Sus scrofa
chemical_list: D011755:Pyrophosphatases; C023255:UDPglucose pyrophosphatase