title: Hemorheology and expression of neutrophil adhesion molecules CD18 and CD62L in pancreatic microcirculation of Caerulein induced experimental acute pancreatitis.
abstract: OBJECTIVE
  To explore the changes in hemorheology and expression of neutrophil adhesion molecules CD18 and CD62L in pancreatic microcirculation of Caerulein induced experimental acute pancreatitis (AP).
  
  
  METHODS
  The Wistar rats (n = 21) were randomized into three groups. The model of AP was established by subcutaneous injection of Caerulein. The changes of apparent viscosity of whole blood were measured by Low- shear 30 rheometer. The expression of adhesion molecules on the surface of neutrophil in duced by shear stress was used with stationary control. CD18 expression was increased on neutrophils treated with shear rate, and andanalyzed using flow cytometry.
  
  
  RESULTS
  Rat treated with Caerulein showed hyperamyleimia (t = 69.029, t = 79.734, P < 0.05). Blood viscosity of two AP groups were significantly elevated (0.512 s(-1): t = 10.725, t = 16.945; 5.96 s(-1): t = 12.781, t = 11.992, P < 0.05). Compared with stationary control, CD18 expression was increased on neutrophil treated with shear rate, and significantly induced with shear rate >/= 94.5 s(-1) (94.5 s(-1): t = 7.403, t = 13.323, t = 16.655; 128.5 s(-1): t = 10.092, t = 28.531, t = 24.563, P < 0.05). The expression of CD62L was less sensitive to low shear rate, and began to be down-regulated significantly when the shear rate >/= 94.5 s(-1) (94.5 s(-1): t = 10.687, t = 19.376, t = 12.848; 128.5 s(-1): t = 26.152, t = 48.402, t = 56.814, P < 0.05).
  
  
  CONCLUSIONS
  The changes of apparent viscosity of whole blood, and the effect of fluid shear stress on the expression of neutrophil adhesion molecules CD18, CD62L may play an important role in the pancreatic microcirculatory failure of acute pancreatitis.
authors.affiliation: Department of Hepato-Bili-Pancreato-Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Zongguang
authors.initials: Z
authors.lastname: Zhou
authors.forename: Youqin
authors.initials: Y
authors.lastname: Chen
authors.forename: Yongyang
authors.initials: Y
authors.lastname: Yu
authors.forename: Huaiqing
authors.initials: H
authors.lastname: Chen
mesh_terms: D000208:Acute Disease; D000818:Animals; D002108:Ceruletide; D005434:Flow Cytometry; D018056:Hemorheology; D008833:Microcirculation; D009504:Neutrophils; D010195:Pancreatitis; D017208:Rats, Wistar
chemical_list: D002108:Ceruletide