title: Comparative effect of dietary administration of Lathyrus sativus pulse on behaviour, neurotransmitter receptors and membrane permeability in rats and guinea pigs.
abstract: Neurolathyrism, an upper motor neuron disease, has been thought to be caused by long-term dietary consumption of lathyrus pulse, which contains the toxin beta-N-oxalyl-L-alpha,beta-diaminopropionic acid. Earlier behavioural studies employing oral feeding of lathyrus pulse to animals has been conducted without evaluating the biochemical toxicity potential. In the present investigation the effect of dietary feeding of 10%, 50% and 80% lathyrus pulse to rats and guinea pigs for 3 months on neurobehavioural parameters, including locomotor activity, inclined plain test and neurotoxicological parameters such as neurotransmitter receptor binding, Ca(2+) influx and membrane fluidity, was investigated. Exposure of 50% low and high toxin lathyrus to rats did not cause any significant change in locomotor activity, whereas guinea pigs at the same dosage regimen of high toxin lathyrus showed significant lowering of inclined plain test scores. Furthermore, studies of neuroreceptor binding in rats fed 50% low and high toxin lathyrus showed significant changes in glutamate, dopamine and muscarinic receptors, whereas the benzodiazepine receptor elicited no change. Guinea pigs, on the other hand, fed 50% and 80% lathyrus in the diet showed significant changes in glutamate, dopamine, muscarinic and benzodiazepine receptors. Interestingly, significant elevation in intracellular calcium with a concomitant increase in membrane fluidity was observed in rats (50% low and high toxin) and guinea pigs (50% and 80%) fed a lathyrus diet. These results indicate that although both species (rats and guinea pigs) are susceptible to neurochemical changes on exposure to lathyrus, locomotor changes are only noticed in guinea pigs. Thus, guinea pigs may be more prone to lathyrus toxicity and may serve as a sensitive animal model compared with rats.
authors.affiliation: Food Toxicology Laboratory, Industrial Toxicology Research Centre, M.G. Marg, Post Box 80, Lucknow 226001, India.
authors.forename: Archana
authors.initials: A
authors.lastname: Amba
authors.forename: Kavita
authors.initials: K
authors.lastname: Seth
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ali
authors.forename: Mukul
authors.initials: M
authors.lastname: Das
authors.forename: A K
authors.initials: AK
authors.lastname: Agarwal
authors.forename: Subhash K
authors.initials: SK
authors.lastname: Khanna
authors.forename: P K
authors.initials: PK
authors.lastname: Seth
mesh_terms: D000284:Administration, Oral; D000599:Amino Acids, Diamino / Q000032:analysis; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002118:Calcium / Q000378:metabolism; D004032:Diet; D006168:Guinea Pigs; D066298:In Vitro Techniques; D027807:Lathyrus / Q000737:chemistry / Q000633:toxicity*; D008560:Membrane Fluidity / Q000187:drug effects; D009498:Neurotoxins / Q000032:analysis; D011869:Radioligand Assay; D051381:Rats; D011954:Receptors, Dopamine / Q000187:drug effects / Q000378:metabolism; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism; D017470:Receptors, Glutamate / Q000187:drug effects / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism; D017981:Receptors, Neurotransmitter / Q000187:drug effects* / Q000378:metabolism; D013045:Species Specificity
chemical_list: D000599:Amino Acids, Diamino; D009498:Neurotoxins; D011954:Receptors, Dopamine; D011963:Receptors, GABA-A; D017470:Receptors, Glutamate; D011976:Receptors, Muscarinic; D017981:Receptors, Neurotransmitter; C100126:oxalyldiaminopropionic acid; D002118:Calcium