Effect of estradiol and progesterone on calcitonin expression in in vitro cultured human endometrial epithelial cells in the implantation window.
OBJECTIVE: To explore the effect of estradiol and progesterone on the expression of calcitonin in human endometrial epithelial cells cultured in vitro. METHODS: Human endometrial epithelial cells were cultured in vitro in DMEM medium. After the cells were treated respectively with estradiol and progesterone at different concentrations for 24 and 48 h, the fluorescence density of the cells was measured by flow cytometer. RESULTS: Progesterone treatment of the epithelial cells for 24 h produced in a dose-dependent manner significantly higher fluorescence level than that of the control (P<0.01). Small dose of estradiol took little effect on calcitonin expression, but high-concentration estradiol resulted in lowered fluorescence level than the control. CONCLUSION: Progesterone promotes calcitonin expression in cultured human endometrial epithelial cells in a dose-dependent manner, while estradiol tends to inhibit its expression.
Fu
Zhi-Hong
ZH
Department of Obstetrics and Gynecology, Nangfang Hospital, First Military Medical University, Guangzhou 510515, China.
Chen
Shi-Ling
SL
Xing
Fu-Qi
FQ