[Clinical significance of fluoroscopic patterns specific for the mitotic spindle in patients with rheumatic diseases].
OBJECTIVE
To determine the clinical significance of anti-NuMA and anti-HsEg5 antibodies in a group of patients affected with rheumatic diseases.


METHODS
Indirect immunofluorescence on HEp-2000 cells at serum dilution of 1:40 was used to examine 26 sera which had previously showed a "mitotic spindle" fluoroscopic pattern type during laboratory routine.


RESULTS
21 sera (80,7%) were identified with NuMA and 5 (19,3%) with HsEg5 patterns alone or associated with other ANA patterns. However only patients with isolated positivity and that is 15 with NuMA and 4 with HsEg5 stainings were included in this study. Of the NuMA positive patients 5 were affected with arthropathies associated to different forms of thyroiditis, 2 with seronegative arthritis, 2 with antiphospholipid syndrome, 1 with systemic lupus erythematosus (SLE), 1 with rheumatoid arthritis, 1 with sicca syndrome, 1 with undifferentiated connective tissue disease, 1 with Mycoplasma pneumoniae infection and 1 with retinal thrombosis. Of the HsEg5 positive patients 3 were affected with SLE and 1 with seronegative arthritis.


CONCLUSIONS
NuMA does not prevail in any defined rheumatic disease, while HsEg5 staining were more frequent (75%) in patients affected with SLE all of whom showing high antibody titres.
Grypiotis
P
P
Cattedra e Divisione di Reumatologia, Università degli Studi di Padova. panagiotis.grypiotis@unipd.it
Ruffatti
A
A
Tonello
M
M
Winzler
C
C
Radu
C
C
Zampieri
S
S
Favaro
M
M
Calligaro
A
A
Todesco
S
S
D000230:Adenocarcinoma / Q000473:pathology; D000974:Antibodies, Antinuclear / Q000276:immunology; D034961:Antigens, Nuclear; D016736:Antiphospholipid Syndrome / Q000097:blood / Q000276:immunology; D001168:Arthritis / Q000097:blood / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D001327:Autoimmune Diseases / Q000097:blood / Q000276:immunology*; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D018385:Centrosome / Q000276:immunology*; D003240:Connective Tissue Diseases / Q000097:blood / Q000276:immunology; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes / Q000032:analysis; D006801:Humans; D016547:Kinesins / Q000032:analysis* / Q000276:immunology; D007822:Laryngeal Neoplasms / Q000473:pathology; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology; D008856:Microscopy, Fluorescence; D034521:Nuclear Matrix-Associated Proteins; D009687:Nuclear Proteins / Q000032:analysis* / Q000276:immunology; D011019:Pneumonia, Mycoplasma / Q000097:blood / Q000276:immunology; D012859:Sjogren's Syndrome / Q000097:blood / Q000276:immunology; D008941:Spindle Apparatus / Q000276:immunology*; D013927:Thrombosis / Q000097:blood / Q000276:immunology; D013967:Thyroiditis, Autoimmune / Q000097:blood / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000648:ultrastructure; D029867:Xenopus Proteins / Q000032:analysis* / Q000276:immunology
D000974:Antibodies, Antinuclear; D034961:Antigens, Nuclear; D001323:Autoantibodies; D018797:Cell Cycle Proteins; D005456:Fluorescent Dyes; C088335:KIF11 protein, Xenopus; C470243:KIF11 protein, human; C489079:NUMA1 protein, Xenopus; C050045:NUMA1 protein, human; D034521:Nuclear Matrix-Associated Proteins; D009687:Nuclear Proteins; D029867:Xenopus Proteins; D016547:Kinesins