Polymerase chain reaction and restriction fragment length polymorphism of cytocrome oxidase subunit I used for differentiation of Brazilian Biomphalaria species intermediate host of Schistosoma mansoni.
The intermediate hosts of Schistosoma mansoni, in Brazil, Biomphalaria glabrata, B. tenagophila and B. straminea, were identified by restriction fragment length polymorphism analysis of the mitochondrial gene cytochrome oxidase I (COI). We performed digestions with two enzymes (AluI and RsaI), previously selected, based on sequences available in Genbank. The profiles obtained with RsaI showed to be the most informative once they were polymorphic patterns, corroborating with much morphological data. In addition, we performed COI digestion of B. straminea snails from Uruguay and Argentina.
Vidigal
Teofânia H D A
TH
Laboratório de Helmintoses Intestinais, Centro de Pesquisas René Rachou, Fiocruz, Belo Horizonte, MG, Brasil.
Montresor
Lângia C
LC
Simpson
Andrew J G
AJ
Carvalho
Omar S
OS
D000818:Animals; D001118:Argentina; D001700:Biomphalaria / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D001938:Brazil; D004272:DNA, Mitochondrial / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics*; D012550:Schistosoma mansoni / Q000502:physiology*; D016622:Silver Staining; D014583:Uruguay
D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; D003576:Electron Transport Complex IV