Purification and crystallization of coconut globulin cocosin from Cocos nucifera.
Cocosin is a legume class reserve protein found in coconut endosperm. Using coconut endosperm, two methods of purification were done. Crystallization was achieved by vapor diffusion (hanging drop) method using MPD, PEG 3350 and PEG 4000 as precipitants. X-ray diffraction data to 3.5-A resolution were collected using Mar345 image plate detector system. Crystals of cocosin grown under 20% MPD, are rhombohedral with space group R3 and cell dimensions a=92.829 A, b=92.829 A, c=215.290 A.
Balasundaresan
D
D
Department of Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600025, India.
Sugadev
R
R
Ponnuswamy
M N
MN
D003059:Cocos / Q000737:chemistry*; D003460:Crystallization; D005916:Globulins / Q000737:chemistry* / Q000302:isolation & purification; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D014961:X-Ray Diffraction / Q000379:methods
D005916:Globulins; D010940:Plant Proteins