Early gene expression associated with the commitment and differentiation of a plant tracheary element is revealed by cDNA-amplified fragment length polymorphism analysis.
Isolated mesophyll cells from Zinnia elegans are induced by auxin and cytokinin to form tracheary elements (TEs) in vitro with high synchrony. To reveal the changing patterns of gene expression during the 48 h of transdifferentiation from mesophyll to TE cell fate, we used a cDNA-amplified fragment length polymorphism approach to generate expression profiles of >30,000 cDNA fragments. Transcriptional changes of 652 cDNA fragments were observed, of which 304 have no previously described function or sequence identity. Sixty-eight genes were upregulated within 30 min of induction and represent key candidates for the processes that underlie the early stages of commitment and differentiation to a TE cell fate.
Milioni
Dimitra
D
Department of Cell and Developmental Biology, John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom.
Sado
Pierre-Etienne
PE
Stacey
Nicola J
NJ
Roberts
Keith
K
McCann
Maureen C
MC
D019659:Asteraceae / Q000235:genetics* / Q000502:physiology; D001692:Biological Transport / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D003583:Cytokinins / Q000494:pharmacology; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007210:Indoleacetic Acids / Q000494:pharmacology; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics*
D003583:Cytokinins; D018076:DNA, Complementary; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins