Transduction of concatemeric plasmids containing the cos site of Lactococcus lactis bacteriophage sk1.
Lactococcus lactis bacteriophage sk1 can transduce plasmids containing the phage cos site and surrounding DNA sequences at frequencies as high as 2x10(-3) transductants per PFU. Deletion analysis demonstrated that the presence of phage DNA spanning cos and putative R sites were the most important for efficient plasmid transduction. Inserts of 440 bp containing cos and the R sites were sufficient to induce transduction frequencies of 10(-4) transductants per PFU. The role of the R1 site was investigated by altering 14 of the 19 bases in the site. This resulted in a two-fold decrease in transduction frequency compared to a 26-fold decrease in transduction following deletion of the entire site. It was demonstrated that transducing plasmids were packaged as linear trimeric concatemers commencing at the cos site.
Chandry
P Scott
PS
Food Science Australia, Werribee, Victoria, Australia. scott.chandry@foodscience.afisc.csiro.au
Moore
Sean C
SC
Davidson
Barrie E
BE
Hillier
Alan J
AJ
D001435:Bacteriophages / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D017353:Gene Deletion; D013294:Lactococcus lactis / Q000235:genetics* / Q000821:virology; D010957:Plasmids / Q000235:genetics*; D014161:Transduction, Genetic
D004279:DNA, Viral