title: Plo1(+) regulates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle.
abstract: The regulation of gene expression plays an important part in cell cycle controls. We describe the molecular machinery that co-ordinates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle. A sequence is identified in the cdc15(+) promoter that we call a PCB (pombe cell cycle box), which confers M-G(1)-specific transcription. Sequences similar to the PCB are present in the promoters of seven other genes, spo12(+), cdc19(+), fin1(+), sid2(+), ppb1(+), mid1(+)/dmf1(+) and plo1(+), which we find to be transcribed at M-G(1). A transcription factor complex is identified that binds to the PCB sequence, which we name PBF, for PCB-binding factor. Finally, we show that PBF binding activity and consequent gene transcription are regulated by the Plo1p protein kinase, thus invoking a potential auto-feedback loop mechanism that regulates mitotic gene transcription and passage through septation and cytokinesis.
authors.affiliation: Division of Biochemistry and Molecular Biology, Institute of Biological and Life Sciences, University of Glasgow, UK.
authors.forename: Mark
authors.initials: M
authors.lastname: Anderson
authors.forename: Szu Shien
authors.initials: SS
authors.lastname: Ng
authors.forename: Vanessa
authors.initials: V
authors.lastname: Marchesi
authors.forename: Fiona H
authors.initials: FH
authors.lastname: MacIver
authors.forename: Frances E
authors.initials: FE
authors.lastname: Stevens
authors.forename: Tracy
authors.initials: T
authors.lastname: Riddell
authors.forename: David M
authors.initials: DM
authors.lastname: Glover
authors.forename: Iain M
authors.initials: IM
authors.lastname: Hagan
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: McInerny
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D016193:G1 Phase; D005800:Genes, Fungal / Q000502:physiology*; D008938:Mitosis / Q000502:physiology*; D012568:Schizosaccharomyces / Q000166:cytology*; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D017931:DNA Primers