Electrophoretically mediated microscale reaction of glycosidases: kinetic analysis of some glycosidases at the nanoliter scale.
Capillary electrophoresis (CE) is one of the extremely important analytical techniques known for its high sensitivity and resolution. We have investigated electrophoretically mediated microanalysis (EMMA) for the assay of some native glycosidases. Under optimized conditions, the enzymatic reactions of alpha-glucosidase, beta-galactosidase and beta-N-acetylglucosaminidase were carried out, and the Michaelis constants were obtained. The current method may have advantages over traditional assay methods, especially in terms of the amount of enzyme and substrate required for a reaction.
Kanie
Yoshimi
Y
Mitsubishi Kagaku Institute of Life Sciences (MLTILS), Machida-shi, 194-8511, Tokyo, Japan.
Kanie
Osamu
O
D000818:Animals; D001331:Automation; D002021:Buffers; D002417:Cattle; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008832:Microchemistry; D010710:Phosphates; D012440:Saccharomyces / Q000201:enzymology; D012680:Sensitivity and Specificity
D002021:Buffers; D010710:Phosphates; D006026:Glycoside Hydrolases