Determination of ketoprofen enantiomers in human serum by capillary zone electrophoresis: man pharmacokinetic studies after administration of rac-ketoprofen tablets.
A rapid and stereospecific capillary zone electrophoresis (CZE) method to quantify ketoprofen (KTP) enantiomers was developed. The KTP enantiomers and (+)-S-naproxen [(+)-S-NPX] as an internal standard (IS) were extracted with methylene chloride from serum acidified. Recovery of both enantiomers was in the range of 85-91%. The enantiomers were determined using a background electrolyte (BGE), consisting of 0.05 M heptakis 2,3,6-tri-O-methyl-beta-cyclodextrin (TMbetaCD) in a phosphate-triethanolamine buffer, which filled a fused silica capillary of 75 micrometer i.d. The linear range of calibration curves was between 0.25 and 50 mg l(-1), with detection limit of 0.1 mg l(-1) (signal-to-noise baseline ratio (S/N) >4). Intra- and interday precision and accuracy of the calibration curves, expressed by the coefficient of variation (CV), did not exceed 15.0%. The validated method has been successfully applied for pharmacokinetic studies of KTP enantiomers from tablets with rac-KTP in man.
Główka
Franciszek K
FK
Department of Physical Chemistry, K. Marcinkowski University of Medical Sciences, 6 Swiecickiego Street, 60-781, Poznań, Poland. glowka@eucalyptus.usoms.poznan.pl
D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D007660:Ketoprofen / Q000031:analogs & derivatives / Q000097:blood* / Q000493:pharmacokinetics*; D013237:Stereoisomerism; D013607:Tablets
D013607:Tablets; D007660:Ketoprofen