Taurine modulates induction of cytochrome P450 3A4 mRNA by rifampicin in the HepG2 cell line.
Taurine is not only present in foods, tonics and nutrient drinks but is also used as a medicinal agent mainly for treatment of chronic heart failure and liver disease. However, little is known about its influence on drug-metabolizing enzymes, especially cytochrome P450 (CYP), in human. We examined whether taurine could affect the expression of CYP3A4 mRNA in the presence or absence of rifampicin (RFP), which is a potent inducer of CYPs, with HepG2 cells. Taurine enhanced twice the induction of CYP3A4 mRNA by RFP, but did not affect the expression by itself. This effect was both concentration- and time-dependent. On the other hand, taurine did not affect the induction by phenobarbital. Taurine did not increase intracellular uptake of RFP. Therefore, we conclude that taurine is an enhancer for the induction of CYP3A4 by RFP.
Matsuda
Hideyasu
H
Clinical Evaluation of Medicines and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Kinoshita
Kayoko
K
Sumida
Akihiko
A
Takahashi
Koichi
K
Fukuen
Shuichi
S
Fukuda
Tsuyoshi
T
Takahashi
Kyoko
K
Yamamoto
Isamu
I
Azuma
Junichi
J
D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis* / Q000187:drug effects; D002460:Cell Line; D051544:Cytochrome P-450 CYP3A; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004790:Enzyme Induction / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D010089:Oxidoreductases, N-Demethylating / Q000096:biosynthesis* / Q000187:drug effects; D010634:Phenobarbital / Q000494:pharmacology; D012293:Rifampin / Q000494:pharmacology*; D013654:Taurine / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors; D015091:beta-Alanine / Q000494:pharmacology
D004791:Enzyme Inhibitors; D015091:beta-Alanine; D013654:Taurine; D001189:Aryl Hydrocarbon Hydroxylases; D051544:Cytochrome P-450 CYP3A; D010089:Oxidoreductases, N-Demethylating; D012293:Rifampin; D010634:Phenobarbital