title: Effect of traumatic brain injury and nitrone radical scavengers on relative changes in regional cerebral blood flow and glucose uptake in rats.
abstract: Changes in regional cerebral blood flow (rCBF) and glucose metabolism are commonly associated with traumatic brain injury (TBI). Reactive oxygen species (ROS) have been implicated as key contributors to the secondary injury process after TBI. Here, pretreatment with the nitrone radical scavengers (alpha-phenyl-N-tert-butyl nitrone (PBN) or its sulfonated analogue sodium 2-sulfophenyl-N-tert-butyl nitrone (S-PBN) were used as tools to study the effects of ROS on rCBF and glucose metabolism after moderate (2.4-2.6 atm) lateral fluid percussion injury (FPI) in rats. S-PBN has a half-life in plasma of 9 min and does not penetrate the blood-brain barrier (BBB). In contrast, PBN has a half-life of 3 h and readily penetrates the BBB. Regional cerebral blood flow (rCBF) and glucose metabolism was estimated by using (99m)Tc-HMPAO and [(18)F]Fluoro-2-deoxyglucose (FDG) autoradiography, respectively, at 42 min (n = 37) and 12 h (n = 34) after the injury. Regions of interest were the parietal cortex and hippocampus bilaterally. As expected, FPI produced an early (42-min) hypoperfusion in ipsilateral cortex and an increase in glucose metabolism in both cortex and hippocampus, giving way to a state of hypoperfusion and decreased glucose metabolism at 12 h postinjury. On the contralateral side, a hypoperfusion in the cortex and hippocampus was seen at 12 h only, but no significant changes in glucose metabolism. Both S-PBN and PBN attenuated the trauma-induced changes in rCBF and glucose metabolism. Thus, the early improvement in rCBF and glucose metabolism correlates with and may partly mediate the improved functional and morphological outcome after TBI in nitrone-treated rats.
authors.affiliation: Department of Neuroscience; Neurosurgery, Uppsala University, Sweden. Niklas.Marklund@neurokir.uu.se
authors.forename: Niklas
authors.initials: N
authors.lastname: Marklund
authors.forename: Sven
authors.initials: S
authors.lastname: Sihver
authors.forename: Bengt
authors.initials: B
authors.lastname: Långström
authors.forename: Mats
authors.initials: M
authors.lastname: Bergström
authors.forename: Lars
authors.initials: L
authors.lastname: Hillered
mesh_terms: D000818:Animals; D001557:Benzenesulfonates / Q000494:pharmacology; D001930:Brain Injuries / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000378:metabolism; D002560:Cerebrovascular Circulation / Q000187:drug effects* / Q000502:physiology; D003497:Cyclic N-Oxides; D019788:Fluorodeoxyglucose F18; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005947:Glucose / Q000493:pharmacokinetics*; D006624:Hippocampus / Q000098:blood supply / Q000000981:diagnostic imaging / Q000378:metabolism; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology; D009589:Nitrogen Oxides / Q000494:pharmacology*; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D019690:Technetium Tc 99m Exametazime
chemical_list: D001557:Benzenesulfonates; D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D009589:Nitrogen Oxides; D019275:Radiopharmaceuticals; D017382:Reactive Oxygen Species; D019788:Fluorodeoxyglucose F18; C094272:N-tert-butyl-(2-sulfophenyl)nitrone; D019690:Technetium Tc 99m Exametazime; C029217:phenyl-N-tert-butylnitrone; D005947:Glucose