Protective effects of polypeptide from Chlamys farreri on Hela cells damaged by ultraviolet A.
OBJECTIVE
To study the protective effect of polypeptide isolated from Chlamys farreri (PCF) on Hela cells damaged by ultraviolet A (UVA) in vitro.


METHODS
Cell proliferation was determined by MTT method; intra-cellular free calcium [Ca2+]i and rates of apoptosis and death were measured by flow cytometry (FCM).


RESULTS
PCF (0.5 %-2 %) enhanced the activities of glutathione peroxidase (GSH-px), superoxide dismutase (SOD), and catalase (CAT), and stimulated cell proliferation. The concentration of [Ca2+]i was increased while the amounts of MDA and the rates of apoptosis and death of the cells were decreased. The differences between the PCF groups and control group were significant (P<0.05, P<0.01).


CONCLUSIONS
PCF protected Hela cells against damage by UVA via its anti-oxidative mechanisms.
Yao
Ru-Yong
RY
Hospital of Medical College, Qingdao University, Qingdao 266003, China.
Wang
Chun-Bo
CB
D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002118:Calcium / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D010125:Ostreidae / Q000737:chemistry*; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays
D000975:Antioxidants; D008419:Materia Medica; D010455:Peptides; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D002118:Calcium