title: The route of passive chloride movement across amphibian skin: localization and regulatory mechanisms.
abstract: Transepithelial Cl(-) conductance (G(Cl)) in amphibian skin can be activated in several species by serosa positive potentials. Mitochondria-rich cells (MRC) or tight junctions (TJ) between the epithelial cells are possible sites for this pathway. The properties and the techniques used to investigate this pathway are reviewed in the present paper. In situ techniques are preferable, since specific properties of the MRC are apparently not maintained in isolated cells. Volume measurements and electronprobe microanalysis of intracellular ions suggest the localization of voltage-activated G(Cl) to MRC. G(Cl) correlates poorly with the density of MRC. The vibrating voltage probe allows quantitative correlation of the local Cl(-) current through morphologically identified structures and the transepithelial Cl(-) current. Our analysis shows that 80% of the voltage-activated Cl(-) current is accounted for by current through MRC or their immediate vicinity. The activation patterns of this current and the inhibition by the alpha(1)-adrenergic agonist, epinephrine, conform to those of the transepithelial current. However, less than 20% of the MRC are active at a certain moment and the activity is spontaneously variable with time. The molecular nature of this pathway, physiological control mechanisms and their relation to the temporal activity of MRC remain to be studied.
authors.affiliation: Physiologisches Institut, Universität München, Schillerstr. 44, München, Germany. W.Nagel@lrz.uni-muenchen.de
authors.forename: Wolfram
authors.initials: W
authors.lastname: Nagel
authors.forename: Petra
authors.initials: P
authors.lastname: Somieski
authors.forename: Uri
authors.initials: U
authors.lastname: Katz
mesh_terms: D000818:Animals; D001001:Anura; D001665:Binding Sites; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D002712:Chlorides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D004553:Electric Conductivity; D017097:Electric Impedance; D004577:Electron Probe Microanalysis; D004837:Epinephrine / Q000494:pharmacology; D004848:Epithelium / Q000737:chemistry / Q000378:metabolism; D007552:Isotonic Solutions; D008928:Mitochondria; D018408:Patch-Clamp Techniques; D000077331:Ringer's Solution; D012867:Skin / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D002712:Chlorides; D007552:Isotonic Solutions; D012965:Sodium Chloride; D000077331:Ringer's Solution; D004837:Epinephrine