title: Different effects of short- and long-chained fructans on large intestinal physiology and carcinogen-induced aberrant crypt foci in rats.
abstract: Inulin-type fructans, which are nondigestible carbohydrates, have been shown to modulate the number of induced preneoplastic lesions in the colon as well as the colonic microflora in laboratory animals. The present study was designed to investigate the effect of a short- and long-chained inulin-type fructan on 1,2-dimethylhydrazine dihydrochloride-induced aberrant crypt foci (ACF) in the rat colon. In addition, the present study investigated the influence of chain length, dietary level (5% or 15%), and duration of feeding (5 or 10 wk) on the following intestinal parameters supposed to be involved in the development of ACF: microflora, short-chain fatty acids, pH, and cell proliferation. A 3-wk pretreatment period with both fructans was included. Feeding the long-chained fructan (5% or 15%) significantly inhibited the numbers of small and total ACF after 5 and 10 wk. The short-chained fructan (15%) inhibited the number of small and total ACF after 5 and 10 wk but significantly increased the numbers of medium and large ACF after 10 wk. In conclusion, the effect on ACF outcome was influenced by the chain length of the fructans.
authors.affiliation: Institute of Food Safety and Toxicology, Danish Veterinary and Food Administration, DK-2860 Søborg, Denmark. mop@fdir.dk
authors.forename: Morten
authors.initials: M
authors.lastname: Poulsen
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Mølck
authors.forename: Bodil Lund
authors.initials: BL
authors.lastname: Jacobsen
mesh_terms: D019813:1,2-Dimethylhydrazine / Q000633:toxicity; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002432:Cecum / Q000737:chemistry / Q000382:microbiology; D002455:Cell Division; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D015169:Colony Count, Microbial; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000382:microbiology; D005630:Fructans / Q000008:administration & dosage*; D006863:Hydrogen-Ion Concentration; D008297:Male; D011230:Precancerous Conditions / Q000139:chemically induced / Q000517:prevention & control*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D005227:Fatty Acids; D005630:Fructans; D019813:1,2-Dimethylhydrazine