title: Cryopreserved cell monolayers for rapid detection of herpes simplex virus and influenza virus.
abstract: Cryopreserved cell monolayers are a new cell culture technology intended to ensure the availability of cells in the laboratory for virus detection. Two cryopreserved cell monolayers, ELVIS for the detection of herpes simplex virus (HSV) and R-Mix for the detection of influenza virus, were evaluated. The results indicated that fresh and cryopreserved cell monolayers are comparable in sensitivity for the detection of HSV and influenza virus. The cells retain the same level of sensitivity for up to 4 months at -80 degrees C.
authors.affiliation: Department of Pathology, University Hospitals of Cleveland, Case Western Reserve University, Cleveland, Ohio 44106, USA. yth@po.cwru.edu
authors.forename: Yung T
authors.initials: YT
authors.lastname: Huang
authors.forename: Huimin
authors.initials: H
authors.lastname: Yan
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Jollick
authors.forename: Heather
authors.initials: H
authors.lastname: Baird
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015925:Cryopreservation; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D009980:Influenza A virus / Q000254:growth & development / Q000302:isolation & purification*; D009981:Influenza B virus / Q000254:growth & development / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013997:Time Factors; D014776:Virus Cultivation / Q000379:methods*
chemical_list: D011933:Reagent Kits, Diagnostic