Changes in Na(+)-K(+)-Cl(-) cotransporter immunoreactivity in the gerbil hippocampus following transient ischemia.
We examined alterations in Na(+)-K(+)-Cl(-) cotransporter 1 (NKCC1) immunoreactivity following ischemia. Twelve hours after ischemia, NKCC1 immunoreactivity in the CA1 region and in the hilar region was significantly diminished. Twenty-four hours after ischemia, NKCC1 immunoreactivity was intensified in these hippocampal regions as well as CA2-3. Two days after ischemia, NKCC1 immunoreactivity in the CA1 and the hilar neurons had disappeared, although in the CA2-3 and the granule cell layer NKCC1 immunoreactivities had recovered to the sham level. This finding suggests that NKCC1 may play an important role in the ischemic neuronal injury induced by excitotoxicity as well as neuronal edema.
Kang
Tae Cheon
TC
Department of Anatomy, College of Medicine, Hallym University, Kangwon-Do 200-702, Chunchon, Republic of Korea.
An
Sung Jin
SJ
Park
Seung Kook
SK
Hwang
In Koo
IK
Yoon
Dae Kun
DK
Shin
Hyo Sun
HS
Won
Moo Ho
MH
D000818:Animals; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D028021:Sodium-Potassium-Chloride Symporters / Q000378:metabolism*; D064506:Solute Carrier Family 12, Member 2; D013997:Time Factors; D015854:Up-Regulation / Q000502:physiology
D028021:Sodium-Potassium-Chloride Symporters; D064506:Solute Carrier Family 12, Member 2