title: Modelling of the human papillomavirus type 16 E5 protein.
abstract: The product of the E5 oncogene in human papillomaviruses (HPVs) participates in cellular transformation. The sequences of E5 from high-risk HPV types are closely related, and the ability to transform is thought to be associated with their structure. Structural determination by standard biophysical methods has proved impossible due to the extreme hydrophobicity of the gene product. We have achieved limited solubility by dividing the sequence into three, structurally distinct domains. Synthetic peptides corresponding to these domains have been examined using circular dichroism (CD) spectroscopy, a method that can detect secondary structure elements in highly dilute protein solutions. Using data on the secondary structure content of these domains under different conditions and in systematic combination to detect constructive domain interactions, a model of HPV E5 structure and position in the membrane is proposed that is consistent with what is known of the larger family of leucine-rich repeat (LRR) proteins to which it belongs.
authors.affiliation: Department of Pathochemistry (0210), Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany.
authors.forename: Angel
authors.initials: A
authors.lastname: Alonso
authors.forename: Jennifer
authors.initials: J
authors.lastname: Reed
mesh_terms: D000595:Amino Acid Sequence; D002472:Cell Transformation, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000737:chemistry*; D027383:Papillomaviridae / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012984:Software; D000086082:Viroporin Proteins
chemical_list: D009856:Oncogene Proteins, Viral; D010446:Peptide Fragments; C054859:oncogene protein E5, Human papillomavirus type 16; D000086082:Viroporin Proteins