title: Gene expression analysis using oligonucleotide arrays produced by maskless photolithography.
abstract: Microarrays containing 195,000 in situ synthesized oligonucleotide features have been created using a benchtop, maskless photolithographic instrument. This instrument, the Maskless Array Synthesizer (MAS), uses a digital light processor (DLP) developed by Texas Instruments. The DLP creates the patterns of UV light used in the light-directed synthesis of oligonucleotides. This digital mask eliminates the need for expensive and time-consuming chromium masks. In this report, we describe experiments in which we tested this maskless technology for DNA synthesis on glass surfaces. Parameters examined included deprotection rates, repetitive yields, and oligonucleotide length. Custom gene expression arrays were manufactured and hybridized to Drosophila melanogaster and mouse samples. Quantitative PCR was used to validate the gene expression data from the mouse arrays.
authors.affiliation: NimbleGen Systems, Inc., Madison, Wisconsin 53711, USA.
authors.forename: Emile F
authors.initials: EF
authors.lastname: Nuwaysir
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Albert
authors.forename: Jaz
authors.initials: J
authors.lastname: Singh
authors.forename: Kate
authors.initials: K
authors.lastname: Nuwaysir
authors.forename: Alan
authors.initials: A
authors.lastname: Pitas
authors.forename: Todd
authors.initials: T
authors.lastname: Richmond
authors.forename: Tom
authors.initials: T
authors.lastname: Gorski
authors.forename: James P
authors.initials: JP
authors.lastname: Berg
authors.forename: Jeff
authors.initials: J
authors.lastname: Ballin
authors.forename: Mark
authors.initials: M
authors.lastname: McCormick
authors.forename: Jason
authors.initials: J
authors.lastname: Norton
authors.forename: Tim
authors.initials: T
authors.lastname: Pollock
authors.forename: Terry
authors.initials: T
authors.lastname: Sumwalt
authors.forename: Lawrence
authors.initials: L
authors.lastname: Butcher
authors.forename: DeAnn
authors.initials: D
authors.lastname: Porter
authors.forename: Michael
authors.initials: M
authors.lastname: Molla
authors.forename: Christine
authors.initials: C
authors.lastname: Hall
authors.forename: Fred
authors.initials: F
authors.lastname: Blattner
authors.forename: Michael R
authors.initials: MR
authors.lastname: Sussman
authors.forename: Rodney L
authors.initials: RL
authors.lastname: Wallace
authors.forename: Franco
authors.initials: F
authors.lastname: Cerrina
authors.forename: Roland D
authors.initials: RD
authors.lastname: Green
mesh_terms: D000818:Animals; D004247:DNA / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D005796:Genes / Q000235:genetics; D017344:Genes, Insect / Q000235:genetics; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry; D010777:Photochemistry / Q000295:instrumentation / Q000379:methods
chemical_list: D015345:Oligonucleotide Probes; D004247:DNA