title: Structural studies of Salmonella typhimurium ArnB (PmrH) aminotransferase: a 4-amino-4-deoxy-L-arabinose lipopolysaccharide-modifying enzyme.
abstract: Lipid A modification with 4-amino-4-deoxy-L-arabinose confers on certain pathogenic bacteria, such as Salmonella, resistance to cationic antimicrobial peptides, including those derived from the innate immune system. ArnB catalysis of amino group transfer from glutamic acid to the 4"-position of a UDP-linked ketopyranose molecule to form UDP-4-amino-4-deoxy-L-arabinose represents a key step in the lipid A modification pathway. Structural and functional studies of the ArnB aminotransferase were undertaken by combining X-ray crystallography with biochemical analyses. High-resolution crystal structures were solved for two native forms and one covalently inhibited form of S. typhimurium ArnB. These structures permitted identification of key residues involved in substrate binding and catalysis, including a rarely observed nonprolyl cis peptide bond in the active site.
authors.affiliation: Structural Genomix, San Diego, CA 92121, USA. brian.noland@stromix.com
authors.forename: Brian W
authors.initials: BW
authors.lastname: Noland
authors.forename: Janet M
authors.initials: JM
authors.lastname: Newman
authors.forename: Jörg
authors.initials: J
authors.lastname: Hendle
authors.forename: John
authors.initials: J
authors.lastname: Badger
authors.forename: Jon A
authors.initials: JA
authors.lastname: Christopher
authors.forename: Jason
authors.initials: J
authors.lastname: Tresser
authors.forename: Michelle D
authors.initials: MD
authors.lastname: Buchanan
authors.forename: Tobi A
authors.initials: TA
authors.lastname: Wright
authors.forename: Marc E
authors.initials: ME
authors.lastname: Rutter
authors.forename: Wendy E
authors.initials: WE
authors.lastname: Sanderson
authors.forename: Hans Joachim
authors.initials: HJ
authors.lastname: Müller-Dieckmann
authors.forename: Ketan S
authors.initials: KS
authors.lastname: Gajiwala
authors.forename: Sean G
authors.initials: SG
authors.lastname: Buchanan
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002384:Catalysis; D018360:Crystallography, X-Ray; D003523:Cycloserine / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D013058:Mass Spectrometry; D008956:Models, Chemical; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011733:Pyridoxamine / Q000031:analogs & derivatives* / Q000737:chemistry; D012486:Salmonella typhimurium / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship; D000637:Transaminases / Q000737:chemistry*
chemical_list: D008070:Lipopolysaccharides; D011733:Pyridoxamine; D003523:Cycloserine; D000637:Transaminases; C010627:pyridoxamine phosphate