Virus replication and cytokine production in dengue virus-infected human B lymphocytes.
Dengue virus (DV) replication, antibody-enhanced viral infection, and cytokine responses of human primary B lymphocytes (cells) were characterized and compared with those of monocytes. The presence of a replication template (negative-strand RNA intermediate), viral antigens including core and nonstructural proteins, and increasing amounts of virus with time postinfection indicated that DV actively replicated in B cells. Virus infection also induced B cells to produce interleukin-6 and tumor necrosis factor alpha, which have been previously implicated in virus pathogenesis. In addition, a heterologous antibody was able to enhance both virus and cytokine production in B cells. Furthermore, the levels of virus replication, antibody-enhanced virus replication, and cytokine responses observed in B cells were not statistically different from those in monocytes. These results suggest that B cells may play an important role in DV pathogenesis.
Lin
Yu-Wen
YW
Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan 70101, Republic of China.
Wang
Kuan-Ju
KJ
Lei
Huan-Yao
HY
Lin
Yee-Shin
YS
Yeh
Trai-Ming
TM
Liu
Hsiao-Sheng
HS
Liu
Ching-Chuan
CC
Chen
Shun-Hua
SH
D000914:Antibodies, Viral / Q000494:pharmacology; D001323:Autoantibodies / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology* / Q000821:virology*; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D003715:Dengue / Q000209:etiology / Q000276:immunology / Q000821:virology; D003716:Dengue Virus / Q000145:classification / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000096:biosynthesis; D009000:Monocytes / Q000276:immunology / Q000821:virology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014779:Virus Replication / Q000276:immunology
D000914:Antibodies, Viral; D001323:Autoantibodies; D016207:Cytokines; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha