title: Mutation analysis of CBP and PCAF reveals rare inactivating mutations in cancer cell lines but not in primary tumours.
abstract: In this study we screened the histone acetyltransferases CBP and PCAF for mutations in human epithelial cancer cell lines and primary tumours. We identified two CBP truncations (both in cell lines), seven PCAF missense variants and four CBP intronic microdeletions. These data suggest that neither gene is commonly inactivated in human epithelial cancers.
authors.affiliation: Cancer Genomics Program, Department of Oncology, University of Cambridge, Hutchison/MRC Research Centre, Cambridge CB2 2XZ, UK.
authors.forename: H
authors.initials: H
authors.lastname: Ozdağ
authors.forename: S J
authors.initials: SJ
authors.lastname: Batley
authors.forename: A
authors.initials: A
authors.lastname: Försti
authors.forename: N G
authors.initials: NG
authors.lastname: Iyer
authors.forename: Y
authors.initials: Y
authors.lastname: Daigo
authors.forename: J
authors.initials: J
authors.lastname: Boutell
authors.forename: M J
authors.initials: MJ
authors.lastname: Arends
authors.forename: B A J
authors.initials: BA
authors.lastname: Ponder
authors.forename: T
authors.initials: T
authors.lastname: Kouzarides
authors.forename: C
authors.initials: C
authors.lastname: Caldas
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D015153:Blotting, Western; D050882:CREB-Binding Protein; D018797:Cell Cycle Proteins / Q000235:genetics; D005091:Exons; D051548:Histone Acetyltransferases; D006801:Humans; D007438:Introns; D009154:Mutation; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors; D014407:Tumor Cells, Cultured; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor
chemical_list: D000123:Acetyltransferases; D050882:CREB-Binding Protein; D018797:Cell Cycle Proteins; D051548:Histone Acetyltransferases; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C494363:CREBBP protein, human