title: Lateral diffusion of cholesterol and dimyristoylphosphatidylcholine in a lipid bilayer measured by pulsed field gradient NMR spectroscopy.
abstract: The pulsed field gradient NMR method for measuring self-diffusion has been used for a direct determination of the lateral diffusion coefficient of cholesterol, fluorine labeled at the 6-position, for an oriented lamellar liquid-crystalline phase of dimyristoylphosphatidylcholine (DMPC)/cholesterol/water. It is found that the diffusion coefficients of DMPC and cholesterol are equal over a large temperature interval. The apparent energy of activation for the diffusion process (58 kJ/mol) is about the same as for a lamellar phase of DMPC/water, whereas the phospholipid lateral diffusion coefficient is approximately four times smaller in the presence of cholesterol.
authors.affiliation: Department of Biophysical Chemistry, Umeå University, SE-901 87 Umeå, Sweden. greger.oradd@chem.umu.se
authors.forename: Greger
authors.initials: G
authors.lastname: Orädd
authors.forename: Göran
authors.initials: G
authors.lastname: Lindblom
authors.forename: Philip W
authors.initials: PW
authors.lastname: Westerman
mesh_terms: D055592:Biophysical Phenomena; D001703:Biophysics; D002784:Cholesterol / Q000737:chemistry*; D004058:Diffusion; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D008051:Lipid Bilayers; D008055:Lipids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013696:Temperature
chemical_list: D008051:Lipid Bilayers; D008055:Lipids; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine