title: Inhibition of natural killer cytotoxicity in vitro by clinical grade serine protease inhibitors.
abstract: The effects of clinical grade serine protease inhibitors on natural killer (NK) activity were compared. Cytotoxicity was measured with the Calcein-AM release method, using K562, Raji as a target. There is a significant correlation between measurements of NK activity by the Calcein-AM method and the 51Cr release assay. Cytotoxicity was inhibited with a calcium chelating agent or a perform inhibitor. Although up to 65% of cytotoxicity was inhibited by nafamostat mesilate with an E/T ratio of 10:1, and by 55% by ulinastatin, neither gabexate mesilate nor antithrombin III inhibited any cytotoxicity. None of these agents inhibited lymphokine-activated killer cell activity. In clinical applications, it should be noted that some protease inhibitors have been proven to have immunosuppressive effects.
authors.affiliation: Blood Transfusion Division, Kobe University Hospital, Japan.
authors.forename: Mitsuteru
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Katsuyasu
authors.initials: K
authors.lastname: Saigo
authors.forename: Masahiro
authors.initials: M
authors.lastname: Koshiba
authors.forename: Noriko
authors.initials: N
authors.lastname: Kitamura
authors.forename: Osamu
authors.initials: O
authors.lastname: Horie
authors.forename: Ryukichi
authors.initials: R
authors.lastname: Ryo
authors.forename: Shunichi
authors.initials: S
authors.lastname: Kumagai
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000990:Antithrombin III / Q000494:pharmacology; D001550:Benzamidines; D002118:Calcium / Q000494:pharmacology; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D005452:Fluoresceins; D016670:Gabexate / Q000494:pharmacology; D006023:Glycoproteins / Q000494:pharmacology; D006146:Guanidines / Q000494:pharmacology; D006801:Humans; D015979:Killer Cells, Lymphokine-Activated / Q000187:drug effects / Q000276:immunology; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D018942:Macrolides; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D000900:Anti-Bacterial Agents; D001550:Benzamidines; D005452:Fluoresceins; D006023:Glycoproteins; D006146:Guanidines; D018942:Macrolides; D015842:Serine Proteinase Inhibitors; C085925:calcein AM; D016670:Gabexate; C036978:concanamycin A; D000990:Antithrombin III; C028665:urinastatin; D002118:Calcium; C032855:nafamostat