title: Virus replication and cytokine production in dengue virus-infected human B lymphocytes.
abstract: Dengue virus (DV) replication, antibody-enhanced viral infection, and cytokine responses of human primary B lymphocytes (cells) were characterized and compared with those of monocytes. The presence of a replication template (negative-strand RNA intermediate), viral antigens including core and nonstructural proteins, and increasing amounts of virus with time postinfection indicated that DV actively replicated in B cells. Virus infection also induced B cells to produce interleukin-6 and tumor necrosis factor alpha, which have been previously implicated in virus pathogenesis. In addition, a heterologous antibody was able to enhance both virus and cytokine production in B cells. Furthermore, the levels of virus replication, antibody-enhanced virus replication, and cytokine responses observed in B cells were not statistically different from those in monocytes. These results suggest that B cells may play an important role in DV pathogenesis.
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan 70101, Republic of China.
authors.forename: Yu-Wen
authors.initials: YW
authors.lastname: Lin
authors.forename: Kuan-Ju
authors.initials: KJ
authors.lastname: Wang
authors.forename: Huan-Yao
authors.initials: HY
authors.lastname: Lei
authors.forename: Yee-Shin
authors.initials: YS
authors.lastname: Lin
authors.forename: Trai-Ming
authors.initials: TM
authors.lastname: Yeh
authors.forename: Hsiao-Sheng
authors.initials: HS
authors.lastname: Liu
authors.forename: Ching-Chuan
authors.initials: CC
authors.lastname: Liu
authors.forename: Shun-Hua
authors.initials: SH
authors.lastname: Chen
mesh_terms: D000914:Antibodies, Viral / Q000494:pharmacology; D001323:Autoantibodies / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology* / Q000821:virology*; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D003715:Dengue / Q000209:etiology / Q000276:immunology / Q000821:virology; D003716:Dengue Virus / Q000145:classification / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000096:biosynthesis; D009000:Monocytes / Q000276:immunology / Q000821:virology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014779:Virus Replication / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D001323:Autoantibodies; D016207:Cytokines; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha