Biotinylated Stealth magnetoliposomes.
Dimyristoylphosphatidylethanolamine (DC(14:0)PE) and the dioleoyl analogue (DC(18:1cis)PE) were mixed with alpha-biotinylamido-omega-N-succinimidoxycarbonyl-poly(ethylene glycol) (NHS-PEG-biotin) and quantitatively converted to alpha-biotinylamido-omega-(dimyristoylphosphatidylethanolamino-carbonyl)polyethylene glycol (DC(14:0)PE-PEG-biotin) and the dioleoyl analogue DC(18:1cis)PE-PEG-biotin, respectively. As shown by thin-layer chromatography and 1H NMR spectroscopy, PEGylation of both phosphatidylethanolamine types went to completion if the reaction was performed in organic solvent in the presence of triethylamine. The resulting derivatives were successfully incorporated into both classical phospholipid vesicles and a phospholipid bilayer surrounding nanometer-sized magnetite cores. In the latter case, the so-called activated Stealth(1) magnetoliposomes were produced which very efficiently immobilized streptavidinylated alkaline phosphatase.
Hodenius
Michael
M
Rheinisch-Westfälische Technische Hochschule Aachen, Prof. Pirlet-Strasse 1, D-52074, Aachen, Germany.
De Cuyper
Marcel
M
Desender
Linda
L
Müller-Schulte
Detlef
D
Steigel
Alois
A
Lueken
Heiko
H
D000469:Alkaline Phosphatase; D001710:Biotin; D016503:Drug Delivery Systems / Q000379:methods*; D052203:Ferrosoferric Oxide; D007501:Iron; D008081:Liposomes / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D010087:Oxides; D011092:Polyethylene Glycols; D019809:Streptavidin
D008081:Liposomes; D010087:Oxides; D011092:Polyethylene Glycols; D001710:Biotin; D019809:Streptavidin; D007501:Iron; D000469:Alkaline Phosphatase; D052203:Ferrosoferric Oxide