[Establishment and characterization of human extrahepatic growing hepatocellular carcinoma cell line EGHC-9901].
OBJECTIVE
To establish a new extrahepatic growing hepatocellular carcinoma cell line.


METHODS
A specimen from extrahepatic growing hepatocellular carcinoma was cultured in vitro. Cancer cells were studied morphologically and subjected to karyotype analysis, DNA analysis, and tumor formation evaluation.


RESULTS
Morphological observation and functional analysis showed that their features were similar to those of HCC. Chromosomes with a variation of 76 approximately 104 were able to secret AFP in vitro and to form bile canaliculi with microvilli.


CONCLUSIONS
EGHC-9901 cell line has characteristics of the extrahepatic growing hepatocellular carcinoma.
Wu
Xiaopeng
X
Department of General Surgery, Qilu Hospital, Shandong University, Ji'nan 250012, China.
Wang
Zhanmin
Z
Liu
Bo
B
Liu
Jun
J
Gao
Yingmao
Y
Li
Zhaoting
Z
Liu
Chunsheng
C
D000328:Adult; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology*; D002869:Chromosome Aberrations; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000032:analysis
D000509:alpha-Fetoproteins