Pre- and postsurgical detection of IgG, IgM, and IgA specific to hydatidosis by ELISA with purified antigen enriched with the 5/B antigen complex.
An enzyme-linked immunoassay (ELISA) using purified 5/B Echinococcus enriched antigen was used to follow IgG, IgM, and IgA antibody levels pre- and posttreatment or surgical removal of hydatid cysts. The sensitivity was 97%, 37.5%, and 54.5%, respectively, and the specificity was 95.7%, 100%, and 98.9%, respectively. All isotypes could be detected 3 years after surgical removal of cysts in patients showing no remaining cyst evidence. This was especially true for IgG, which persisted in 85.2% of the patients. The data indicate that antigen purification improves specificity without affecting sensitivity, although this new antigen offers no advantages in the postsurgical monitoring of the patients.
Doiz
Olga
O
Servicio de Microbiología, Hospital Clínico Universitario, Zaragoza, España.
Benito
Rafael
R
Gil
Joaquina
J
Rojas
Almudena
A
Rubio
M Carmen
MC
Osuna
Antonio
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D000947:Antigens, Helminth / Q000276:immunology; D004443:Echinococcosis / Q000097:blood / Q000276:immunology* / Q000601:surgery; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis* / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis* / Q000276:immunology; D007519:Isoantigens / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period
C451327:5b antigen; D000936:Antigen-Antibody Complex; D000947:Antigens, Helminth; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007519:Isoantigens