title: Cyclic nucleotide content of tobacco BY-2 cells.
abstract: The cyclic nucleotide content of cultured tobacco bright yellow-2 (BY-2) cells was determined, after freeze-killing, perchlorate extraction and sequential chromatography, by radioimmunoassay. The identities of the putative cyclic nucleotides, adenosine 3',5'-cyclic monophosphate (cyclic AMP), guanosine 3',5'-cyclic monophosphate (cyclic GMP) and cytidine 3',5'-cyclic monophosphate (cyclic CMP) were unambiguously confirmed by tandem mass spectrometry. The potential of BY-2 cell cultures as a model system for future investigations of cyclic nucleotide function in higher plants is discussed.
authors.affiliation: Biochemistry Group, Wallace Building, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK.
authors.forename: Helen
authors.initials: H
authors.lastname: Richards
authors.forename: Swadipa
authors.initials: S
authors.lastname: Das
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Smith
authors.forename: Louisa
authors.initials: L
authors.lastname: Pereira
authors.forename: Alan
authors.initials: A
authors.lastname: Geisbrecht
authors.forename: Nicola J
authors.initials: NJ
authors.lastname: Devitt
authors.forename: David E
authors.initials: DE
authors.lastname: Games
authors.forename: Jan
authors.initials: J
authors.lastname: van Geyschem
authors.forename: A
authors.initials: A
authors.lastname: Gareth Brenton
authors.forename: Russell P
authors.initials: RP
authors.lastname: Newton
mesh_terms: D002460:Cell Line; D000242:Cyclic AMP / Q000032:analysis* / Q000302:isolation & purification; D003563:Cyclic CMP / Q000032:analysis* / Q000302:isolation & purification; D006152:Cyclic GMP / Q000032:analysis* / Q000302:isolation & purification; D013058:Mass Spectrometry; D014026:Nicotiana / Q000737:chemistry* / Q000166:cytology*
chemical_list: D003563:Cyclic CMP; D000242:Cyclic AMP; D006152:Cyclic GMP