Biooxidation of n-hexanol by alcohol oxidase and catalase in biphasic and micellar systems without solvent.
Alcohol oxidase from Pichia pastoris together with catalase from bovine liver was used to oxidize n-hexanol to hexanal. For this purpose, an aqueous buffer solution was mixed with large amounts of hexanol by simple agitation, yielding a biphasic system, or by adding the nonionic surfactant Brij 35. Initial velocities and reaction yields after 24 h were measured as a function of various parameters such as the amounts of enzymes, hexanol, or surfactant. High enzymatic activity was determined for hexanol concentrations of between 20 mass% and 80 mass% without using any additional organic solvent. The homogenization of the biphasic systems with the help of Brij 35 did not yield a significant improvement of the bioconversion, which would justify the use of surfactants.
Karra-Chaabouni
Maha
M
Laboratoire de Technologie Enzymatique, Université de Technologie de Compiègne (UTC), France.
Pulvin
Sylviane
S
Meziani
Abdelghani
A
Thomas
Daniel
D
Touraud
Didier
D
Kunz
Werner
W
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism*; D004655:Emulsions; D000441:Hexanols / Q000378:metabolism*; D008823:Micelles; D010084:Oxidation-Reduction; D012997:Solvents / Q000737:chemistry*; D013816:Thermodynamics
D004655:Emulsions; D000441:Hexanols; D008823:Micelles; D012997:Solvents; C036260:1-hexanol; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D002374:Catalase