Cloning and sequence analysis of cDNA and genomic DNA of human thrombopoietin gene.
OBJECTIVE: To study the role of thrombopoietin (TPO) gene 5' untranslated region (UTR) and the intron in TPO expression regulation. METHODS: With the mRNA derived from Chinese human fetal liver as the template, full-length TPO cDNA (approximately 1.1 kb) and TPO genomic DNA(6.2 kb) along with all the introns of TPO gene were isolated by PCR and long-distance PCR techniques from Chinese fetal liver tissue. RESULT: Sequencing analysis indicated that all the coding sequences and the exon/intron splice sites were consistent with the results previously reported by Sohma et al except for only a DNA and all the introns in the intron of TPO gene. CONCLUSION: We have successfully cloned full-length TPO cDNA, TPO genomic DNA and all the introns of TPO gene from Chinese fetal liver tissue.
Ning
Yun-Shan
YS
Institute of Molecular Immunology, First Military Medical University, Guangzhou 510515, China.
Zhou
Hong-Wei
HW
Zhou
Ming-Qian
MQ
Chen
Ze-Hong
ZH
Fang
Xiang-Dong
XD
Fu
Ning
N
Wang
Xiao-Ning
XN