title: Extract and identify ingredient from Ligustrum Lucidum Ait and study its effect to periodontal pathogen.
abstract: OBJECTIVE
  To extract the effective ingredient (crystal I) from effective section (saponin) of Ligustrum Lucidum Ait, identify the chemical structure of crystal I, study the effect of crystal I on P. gingivalis, B. forsythus and P. intermedia.
  
  
  METHODS
  Isolated crystal I from saponin using the silica gel column chromatograph. Identified crystal I with IR spectra, (1)H-NMR and (13)C-NMR. Measured the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) through micro-liquid dilution. Studied the killing curve of ursolic acid on B. forsythus and P. intermedia.
  
  
  RESULTS
  The crystal I was identified as ursolic acid; its MIC and MBC to P. gingivalis, B. forsythus and P. intermedia were 0.740 and 0.295 microg/L respectively. The killing curve indicated that 0.800 microg/L ursolic acid could kill P. intermedia and B. forsythus in 3 and 6 hours respectively.
  
  
  CONCLUSIONS
  Ursolic acid has obvious effect to inhibit periodontal pathogen.
authors.affiliation: Department of Operative Dentistry and Endodontics, School of Stomatology, Wuhan University, Wuhan 430079, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Mingwen
authors.initials: M
authors.lastname: Fan
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Bian
authors.forename: Min
authors.initials: M
authors.lastname: Nie
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
mesh_terms: D001439:Bacteroides / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D031659:Ligustrum; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008826:Microbial Sensitivity Tests; D010519:Periodontium / Q000382:microbiology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D016966:Porphyromonas gingivalis / Q000187:drug effects; D018713:Prevotella intermedia / Q000187:drug effects; D014315:Triterpenes / Q000494:pharmacology; D000097245:Ursolic Acid
chemical_list: D010936:Plant Extracts; D014315:Triterpenes; D000097245:Ursolic Acid