Observations on the feeding behaviour of Paratrichodorus anemones in relation to tobravirus transmission.
Feeding by P. anemones, an efficient vector of tobacco rattle virus (TRV), was investigated by video-enhanced interference light microscopy. Four stages were observed after transfer of individual nematodes, extracted from soil, to Nicotiana tabacum seedling roots in agar: i) acclimatisation; ii) approach and scrutiny; iii) preparation; and iv) feeding. Prior to commencement of stage 'iv' about 4 cells perforated by rapid onchiostyle thrusting remained alive, each having been almost immediately abandoned by the nematode. During the stage iv) approximately 5% of perforated cells remained alive. Feeding on individual cells was similar to that previously reported for Trichodorus similis: cells from which cytoplasm was ingested after a prolonged period of salivation were invariably killed, with adjacent cells being unaffected. During feeding a number of cells perforated but soon afterwards abandoned by P. anemones remained alive, providing an effective pathway for successful transmission of TRV to plants by the nematode.
Karanastasi
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E
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK.
Wyss
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U
Brown
D
D
D000818:Animals; D004199:Disease Vectors; D005247:Feeding Behavior; D018715:Microscopy, Video / Q000662:veterinary; D010935:Plant Diseases / Q000469:parasitology / Q000821:virology*; D018517:Plant Roots / Q000469:parasitology / Q000821:virology; D010942:Plant Viruses / Q000502:physiology; D012328:RNA Viruses / Q000502:physiology*; D014026:Nicotiana / Q000469:parasitology / Q000821:virology; D017187:Tylenchida / Q000502:physiology* / Q000821:virology*