Short communication: characterization of a new genetic variant in the caprine kappa-casein gene.
A new polymorphism has been identified in the goat kappa-casein gene by evaluating genomic DNA from the Montefalcone breed in Italy. The polymorphic site consists of a single nucleotide substitution A to G at position 242 of the exon 4 and produces an amino acid substitution Asp/Gly. A polymerase chain reaction-restriction fragment length polymorphism protocol for rapid genotyping of the variant has been developed, using the HaeIII enzyme. Animals from Italian, Spanish, and French breeds have been analyzed to investigate the occurrence of the allele in other populations. The allele appears to be exclusive to the Montefalcone breed.
Angiolillo
A
A
Dipartimento di Scienze Animali, Vegetali e dell'Ambiente, Università del Molise, Campobasso, Italy. angiolillo@unimol.it
Yahyaoui
M H
MH
Sánchez
A
A
Pilla
F
F
Folch
J M
JM
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002364:Caseins / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D014644:Genetic Variation; D006041:Goats / Q000235:genetics*; D007558:Italy; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D002364:Caseins; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases