Antioxidant activity in common beans (Phaseolus vulgaris L.).
Beans were pearled to evaluate the feasibility of increasing antioxidant activity and phenolic antioxidants. Phenolics were concentrated mostly in the hull fraction at about 56 mg of catechin equivalents per gram of sample. The methanolic extracts of the pearled bean samples were screened for antioxidant potential using the beta-carotene-linoleate and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) in vitro model systems. The pearled material, also referred to as milled samples, exhibited antioxidant activity that correlated with phenolic content and inhibited DPPH significantly in a dose-dependent manner. Phenolics and antioxidant activities were also examined in chromatographic fractions of methanolic extracts of manually obtained hulls that represented a model used previously to ascertain antimutagenic activity. Fractions extracted with ethyl acetate/acetone and acetone displayed antioxidant activity, which implies potent free radical scavenging activity with antimutagenic activity.
Cardador-Martínez
Anaberta
A
Programa de Posgrado en Alimentos del Centro de la República (PROPAC), Research and Graduate Studies in Food Science, School of Chemistry, Universidad Autónoma de Querétaro, Querétaro, Qro., 76010 Mexico.
Loarca-Piña
Guadalupe
G
Oomah
B Dave
BD
D000085:Acetates; D000096:Acetone; D016587:Antimutagenic Agents; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D007700:Kinetics; D019787:Linoleic Acid / Q000737:chemistry; D000432:Methanol; D027805:Phaseolus / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D019207:beta Carotene / Q000737:chemistry
D000085:Acetates; D016587:Antimutagenic Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D019207:beta Carotene; D000096:Acetone; C007650:ethyl acetate; D019787:Linoleic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol