Functional characterization of the human spermidine/spermine N(1)-acetyltransferase gene promoter.
Spermidine/spermine N(1)-acetyltransferase (SSAT), the key enzyme of polyamine catabolism, is induced by antiproliferative stresses. We analyzed the 5' flanking region of the human SSAT gene, and clarified that the binding of Sp1 to the GC-box located 42 to 51 bp upstream from the transcription start site is essential for transcription in HeLa S3 cells. A polyamine-responsive element (PRE) seemed to be responsible for the elevated transcription after X-ray irradiation.
Tomitori
Hideyuki
H
Department of Clinical Biochemistry, Graduate School of Pharmaceutical Sciences, Chiba University, 1-33, Yayoi-cho, Inage-ku, Chiba, Japan.
Nenoi
Mitsuru
M
Mita
Kazuei
K
Daino
Kazuhiro
K
Igarashi
Kazuei
K
Ichimura
Sachiko
S
D024506:5' Flanking Region; D000123:Acetyltransferases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D018983:DNA Footprinting; D004268:DNA-Binding Proteins; D004307:Dose-Response Relationship, Radiation; D024202:Electrophoretic Mobility Shift Assay; D006367:HeLa Cells / Q000528:radiation effects; D006801:Humans; D017354:Point Mutation; D011401:Promoter Regions, Genetic / Q000502:physiology* / Q000528:radiation effects; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D016329:Sp1 Transcription Factor / Q000378:metabolism; D013095:Spermidine / Q000378:metabolism*; D013096:Spermine / Q000378:metabolism*; D013997:Time Factors; D024363:Transcription Initiation Site; D014158:Transcription, Genetic / Q000528:radiation effects; D015854:Up-Regulation; D000099228:Diamine N-Acetyltransferase
D000123:Acetyltransferases; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D016329:Sp1 Transcription Factor; D013095:Spermidine; D013096:Spermine; D000099228:Diamine N-Acetyltransferase