title: Characterization of the antigenic, immunogenic, and pathogenic variation of infectious bursal disease virus due to propagation in different host systems (bursa, embryo, and cell culture). III. Pathogenicity.
abstract: Differences in the relative pathogenicity of variant (1084 E and GLS) and standard (Edgar and STC) infectious bursal disease virus (IBDV) strains were observed after propagation in the bursa of Fabricius, embryos, or cell cultures. Bursa-derived IBDV induced the most severe lesions in the bursa of Fabricius when compared with strains propagated in embryos or cell cultures. Embryo-derived IBDV induced moderate gross bursal lesions, whereas cell culture-derived IBDV did not damage the bursa grossly. A high frequency of virus re-isolations was obtained from bursal, spleen, and thymic samples collected from birds inoculated with bursa-derived or embryo-derived IBDV. Virus re-isolation occurred much less frequently from birds inoculated with cell culture-adapted IBDV. Serological evaluations demonstrated that bursa-derived IBDV strains induced a higher neutralizing antibody response than did embryo-derived or cell culture-derived strains. These results document that the relative pathogenicity and immunogenicity of IBDV is reduced following propagation in embryos or cell cultures.
authors.affiliation: Department of Animal and Food Sciences, Allen Biotechnology Laboratory, University of Delaware, 601 Sincock Lane, Newark, DE 19717, USA. isaacrch@lycos.com
authors.forename: Isaac R
authors.initials: IR
authors.lastname: Rodriguez-Chavez
authors.forename: John K
authors.initials: JK
authors.lastname: Rosenberger
authors.forename: Sandra S
authors.initials: SS
authors.lastname: Cloud
authors.forename: Conrad R
authors.initials: CR
authors.lastname: Pope
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D002060:Bursa of Fabricius / Q000276:immunology* / Q000473:pathology; D002478:Cells, Cultured; D002642:Chick Embryo / Q000821:virology*; D002645:Chickens; D007243:Infectious bursal disease virus / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D011201:Poultry Diseases / Q000276:immunology / Q000473:pathology; D014774:Virulence; D014779:Virus Replication