title: 5' exonuclease assay for detection of serogroup Y Neisseria meningitidis.
abstract: The incidence of serogroup Y meningococcal disease has increased recently in the United States. Here, we describe the development of a 5' exonuclease assay for the detection of serogroup Y Neisseria meningitidis and demonstrate the usefulness of this assay for resolving serogroup identification of strains that are resistant to conventional serogrouping and for the nonculture identification of serogroup Y meningococcal disease.
authors.affiliation: Microbial Diseases Laboratory, Division of Communicable Disease Control, California Department of Health Services, Berkeley, California 94704-1011, USA. wprobert@dhs.ca.gov
authors.forename: William S
authors.initials: WS
authors.lastname: Probert
authors.forename: Susan L
authors.initials: SL
authors.lastname: Bystrom
authors.forename: Shideh
authors.initials: S
authors.lastname: Khashe
authors.forename: Kimmi N
authors.initials: KN
authors.lastname: Schrader
authors.forename: Jane D
authors.initials: JD
authors.lastname: Wong
mesh_terms: D001769:Blood / Q000382:microbiology; D002555:Cerebrospinal Fluid / Q000382:microbiology; D005092:Exonucleases / Q000235:genetics*; D006801:Humans; D008589:Meningococcal Infections / Q000175:diagnosis / Q000382:microbiology*; D038581:Neisseria meningitidis, Serogroup Y / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012703:Serotyping; D012799:Sialyltransferases / Q000235:genetics
chemical_list: D012799:Sialyltransferases; D005092:Exonucleases