title: Regulation of freA, acoA, lysF, and cycA expression by iron availability in Aspergillus nidulans.
abstract: In the filamentous fungus Aspergillus nidulans, iron homeostasis is regulated at the transcriptional level by the negative-acting GATA factor SREA. In this study the expression of a putative heme-containing metalloreductase-encoding gene, freA, was found to be upregulated by iron limitation independently of SREA, demonstrating the existence of an iron-regulatory mechanism which does not involve SREA. In contrast to freA, various other genes encoding proteins in need of iron-containing cofactors-acoA, lysF, and cycA-were downregulated in response to iron depletion. Remarkably, SREA deficiency led to increased expression of acoA, lysF, and cycA under iron-replete growth conditions.
authors.affiliation: Department of Molecular Biology. Institute of Legal Medicine, University of Innsbruck, A-6020 Innsbruck, Austria.
authors.forename: Harald
authors.initials: H
authors.lastname: Oberegger
authors.forename: Michelle
authors.initials: M
authors.lastname: Schoeser
authors.forename: Ivo
authors.initials: I
authors.lastname: Zadra
authors.forename: Markus
authors.initials: M
authors.lastname: Schrettl
authors.forename: Walther
authors.initials: W
authors.lastname: Parson
authors.forename: Hubertus
authors.initials: H
authors.lastname: Haas
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D006836:Hydro-Lyases / Q000096:biosynthesis; D007501:Iron / Q000502:physiology*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D017386:Sequence Homology, Amino Acid
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D007501:Iron; C469336:FreA protein, Aspergillus nidulans; D010088:Oxidoreductases; D006836:Hydro-Lyases; C107855:homoaconitate hydratase