Phase II enzyme-inducing and antioxidant activities of beetroot (Beta vulgaris L.) extracts from phenotypes of different pigmentation.
Free-radical scavenging, reducing, and phase II enzyme-inducing activities of aqueous and 5% aqueous ethanol extracts of freeze-dried root tissue of four beet (Beta vulgaris L.) strains (red, white, orange, and high-pigment (red) phenotypes) were determined. Aqueous and ethanolic tissue extracts of the regular and high-pigment red phenotypes were most capable of inhibiting metmyoglobin/H(2)O(2)-mediated oxidation of 2-2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 2,2'-azobis-(2-amidinopropane) dihydrochloride (AAPH)-mediated bleaching of beta-carotene. These same extracts were also most efficient at reducing ABTS radical cation and inducing quinone reductase in murine hepatoma (Hepa 1c1c7) cells in vitro.
Wettasinghe
Mahinda
M
Department of Food Science, 1605 Linden Drive, Babcock Hall, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Bolling
Bradley
B
Plhak
Leslie
L
Xiao
Hang
H
Parkin
Kirk
K
D000578:Amidines / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D052160:Benzothiazoles; D027461:Beta vulgaris / Q000737:chemistry*; D004790:Enzyme Induction / Q000187:drug effects*; D000431:Ethanol; D005609:Free Radicals / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D008114:Liver Neoplasms, Experimental; D008786:Metmyoglobin / Q000737:chemistry; D051379:Mice; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000096:biosynthesis; D010641:Phenotype; D010860:Pigments, Biological / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D013451:Sulfonic Acids / Q000737:chemistry; D014407:Tumor Cells, Cultured; D014867:Water; D019207:beta Carotene / Q000737:chemistry
D000578:Amidines; D000975:Antioxidants; D052160:Benzothiazoles; D005609:Free Radicals; D010860:Pigments, Biological; D010936:Plant Extracts; D013451:Sulfonic Acids; D019207:beta Carotene; D014867:Water; D008786:Metmyoglobin; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D000431:Ethanol; C046728:2,2'-azobis(2-amidinopropane); D006861:Hydrogen Peroxide; D016660:NAD(P)H Dehydrogenase (Quinone)