Structural elucidation of the EPS of slime producing Brevundimonas vesicularis sp. isolated from a paper machine.
The slime forming bacteria Brevundimonas vesicularis sp. was isolated from a paper mill and its EPS was produced on laboratory scale. After production, the exopolysaccharide (EPS) was purified and analysed for its purity and homogeneity, HPSEC revealed one distinct population with a molecular mass of more than 2,000 kDa. The protein content was around 9 w/w%. The sample was analysed to determine its chemical structure. The EPS was found to consist of rhamnose, glucose, galacturonic acid and glucuronic acid. Due to the presence of uronic acids the molar ratio between the four sugars found varies from 3:5:2:4 by sugar composition analyses after methanolysis to 1:1:1:1 found by NMR. A repeating unit with a molecular mass of 678 Da was confirmed by MALDI-TOF mass spectrometry after mild acid treatment. 13C and 1H hetero- and homonuclear 2D NMR spectroscopy of the native and partial hydrolysed EPS revealed a repeating unit, no non-sugar substituents were present.
Verhoef
René
R
Wageningen University, Department of Agrotechnology and Food Sciences, Laboratory of Food Chemistry, Bomenweg 2, NL-6703 HD Wageningen, The Netherlands.
de Waard
Pieter
P
Schols
Henk A
HA
Rättö
Marjaana
M
Siika-aho
Matti
M
Voragen
Alphons G J
AG
D020561:Alphaproteobacteria / Q000737:chemistry* / Q000302:isolation & purification; D002240:Carbohydrate Sequence; D007218:Industrial Microbiology / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010209:Paper; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D011135:Polysaccharides, Bacterial