Effect of transient hippocampal inhibition on amygdaloid kindled seizures and amygdaloid kindling rate.
In this study the effect of transient inhibition of the CA1 region of the dorsal hippocampus by lidocaine on amygdala kindling rate and amygdaloid kindled seizures was investigated. In experiment 1, rats were divided into four groups. In group 1, animals were implanted only with a tripolar electrode into the amygdala but in groups 2-4, two guide cannulae were also implanted into the CA1 regions of the dorsal hippocampi. Animals were stimulated daily to be kindled. In groups 3 and 4, saline or 2% lidocaine (1 microl/2 min) was also injected respectively into the hippocampus, 5 min before each stimulation. Results obtained showed that amygdala kindling rate and the number of stimulations to receive from stage 4 to stage 5 seizure were significantly increased in group 4. In experiment 2, lidocaine (1% and 2%) was infused (1 microl/2 min) into the hippocampus of amygdala kindled rats bilaterally and animals were stimulated at 5, 15 and 30 min after drug injection. Twenty four h before lidocaine injection, saline was also infused (1 microl/2 min) into the hippocampus as control. Obtained results showed that afterdischarge duration was reduced 5 min after lidocaine (1% and 2%) injection. Stage 5 seizure duration was also decreased 5 and 15 min after 2% lidocaine. Thus, it may be suggested that in amygdala kindling, activation of the hippocampal CA1 region has a role in seizure acquisition and seizure severity so that inhibition of this region results in decreasing of seizure severity and retards amygdala kindling rate.
Mirnajafi-Zadeh
Javad
J
Department of Physiology, School of Medical Sciences, Tarbiat Modarres University, Tehran, Iran. mirnajaf@modares.ac.ir
Mortazavi
Mostafa
M
Fathollahi
Yaghoub
Y
Alasvand Zarasvand
Masoud
M
Reza Palizvan
Mohammad
M
D000679:Amygdala / Q000502:physiology* / Q000503:physiopathology; D000818:Animals; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology* / Q000503:physiopathology; D007696:Kindling, Neurologic / Q000502:physiology*; D008012:Lidocaine / Q000494:pharmacology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000503:physiopathology; D026941:Sodium Channel Blockers / Q000494:pharmacology
D026941:Sodium Channel Blockers; D008012:Lidocaine