title: Increase of separation resolution through field enhancement in microchips.
abstract: An enhanced ability to separate charged species from neutral compounds in a microfluidic chip is demonstrated using a chip design with low-resistance electrode channels operating with a multiport pressure/voltage controller. A factor of 2.7 improvement in resolution was obtained from chips made using identical mask designs but different etch depth protocols. Greater separation power allows one to cover a wider dynamic range for compounds with different electrophoretic mobilities.
authors.affiliation: Caliper Technologies Corp., 605 Fairchild Drive, Mountain View, CA 94043, USA.
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Kerby
authors.forename: Ring-Ling
authors.initials: RL
authors.lastname: Chien
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives / Q000302:isolation & purification; D001896:Boron Compounds / Q000302:isolation & purification; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D008904:Miniaturization
chemical_list: D001896:Boron Compounds; D001120:Arginine