title: Osseointegration on metallic implant surfaces: effects of microgeometry and growth factor treatment.
abstract: Orthopedic implants often loosen due to the invasion of fibrous tissue. The aim of this study was to devise a novel implant surface that would speed healing adjacent to the surface, and create a stable interface for bone integration, by using a chemoattractant for bone precursor cells, and by controlling tissue migration at implant surfaces via specific surface microgeometry design. Experimental surfaces were tested in a canine implantable chamber that simulates the intramedullary bone response around total joint implants. Titanium and alloy surfaces were prepared with specific microgeometries, designed to optimize tissue attachment and control fibrous encapsulation. TGF beta, a mitogen and chemoattractant (Hunziker EB, Rosenberg LC. J Bone Joint Surg Am 1996;78:721-733) for osteoprogenitor cells, was used to recruit progenitor cells to the implant surface and to enhance their proliferation. Calcium sulfate hemihydrate (CS) was the delivery vehicle for TGF beta; CS resorbs rapidly and appears to be osteoconductive. Animals were sacrificed at 6 and 12 weeks postoperatively. Results indicated that TGFbeta can be reliably released in an active form from a calcium sulfate carrier in vivo. The growth factor had a significant effect on bone ingrowth into implant channels at an early time period, although this effect was not seen with higher doses at later periods. Adjustment of dosage should render TGF beta more potent at later time periods. Calcium sulfate treatment without TGF beta resulted in a significant increase in bone ingrowth throughout the 12-week time period studied. Bone response to the microgrooved surfaces was dramatic, causing greater ingrowth in 9 of the 12 experimental conditions. Microgrooves also enhanced the mechanical strength of CS-coated specimens. The grooved surface was able to control the direction of ingrowth. This surface treatment may result in a clinically valuable implant design to induce rapid ingrowth and a strong bone-implant interface, contributing to implant longevity.
authors.affiliation: Department of Orthopaedic Surgery, Musculoskeletal Research Center, New York University Hospital for Joint Diseases, New York, New York 10003, USA. sally.frankel@excite.com
authors.forename: Sally R
authors.initials: SR
authors.lastname: Frenkel
authors.forename: Jordan
authors.initials: J
authors.lastname: Simon
authors.forename: Harold
authors.initials: H
authors.lastname: Alexander
authors.forename: Michael
authors.initials: M
authors.lastname: Dennis
authors.forename: John L
authors.initials: JL
authors.lastname: Ricci
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002133:Calcium Sulfate; D004285:Dogs; D008422:Materials Testing; D008670:Metals; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration / Q000187:drug effects; D019736:Prostheses and Implants; D011994:Recombinant Proteins / Q000008:administration & dosage; D013314:Stress, Mechanical; D013499:Surface Properties; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000008:administration & dosage*
chemical_list: D001672:Biocompatible Materials; D008670:Metals; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D002133:Calcium Sulfate