Expression and folding of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm.
In this review, we summarize some of our results on folding and directed evolution of an antibody fragment in Escherichia coli cytoplasm. We will also discuss some attempts to construct other antibodies active in this cellular compartment.
Laden
Jean-Charles
JC
CNRS UMR5094, Faculté de Pharmacie, Montpellier, France.
Philibert
Pascal
P
Torreilles
François
F
Pugnière
Martine
M
Martineau
Pierre
P
D003593:Cytoplasm / Q000378:metabolism*; D019020:Directed Molecular Evolution; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000378:metabolism*; D017510:Protein Folding
D007128:Immunoglobulin Fragments; C055966:immunoglobulin Fv