Changes in Na(+)-K(+)-Cl(-) cotransporter immunoreactivity in the gerbil hippocampus following spontaneous seizure.
The immunoreactivity of Na(+)-K(+)-Cl(-) cotransporter (NKCC) in the gerbil hippocampus associated with various sequelae of spontaneous seizures were investigated in order to identify the roles of NKCC in the epileptogenesis and the recovery mechanisms in these animals. The NKCC immunoreactivities in the CA2-3 regions, the subiculum and the entorhinal cortex, were significantly more intensified in the pre-seizure group of seizure sensitive (SS) gerbils than in the seizure resistant (SR) gerbils. Following the on-set of seizure, the immunoreactivity of NKCC was significantly changed. In the hippocampal complex except the CA1 region, NKCC immunoreactivity in GABAergic neurons was significantly decreased 30 min after seizure on-set, versus the pre-seizure group. On the other hand, NKCC immunoreactivity was dramatically elevated in the CA1 regions, and 3 h postictal NKCC immunoreactivity increased significantly in the dentate gyrus and the dendrites of the pyramidal cells in the CA2-3 regions. These findings suggest that altered NKCC expression may be associated with seizure activity, and have an important role in the postictal recovery by regulating GABA-mediated inhibitory circuit in the hippocampal complex of the gerbil.
Kang
Tae-Cheon
TC
Department of Anatomy, College of Medicine, Hallym University, 200-702 Kangwon-Do, Chunchon, South Korea. tckang@hallym.ac.kr
An
Sung-Jin
SJ
Park
Seung-Kook
SK
Hwang
In-Koo
IK
Bae
Jae Chun
JC
Suh
Jun-Gyo
JG
Oh
Yang-Seok
YS
Won
Moo Ho
MH
D000818:Animals; D018891:Dentate Gyrus / Q000378:metabolism; D018728:Entorhinal Cortex / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry; D012640:Seizures / Q000378:metabolism*; D028021:Sodium-Potassium-Chloride Symporters / Q000378:metabolism*
D028021:Sodium-Potassium-Chloride Symporters