Structure of the O-specific polysaccharide of Providencia alcalifaciens O16 containing N-acetylmuramic acid.
The O-specific polysaccharide of Providencia alcalifaciens O16 was obtained by mild-acid degradation of the lipopolysaccharide and studied by chemical methods and NMR spectroscopy, including 2D 1H,(1)H COSY, TOCSY, NOESY, and 1H,(13)C HSQC experiments. It was found that the polysaccharide contains N-acetylmuramic acid, which was isolated by solvolysis with trifluoromethanesulfonic acid and identified by the specific optical rotation and NMR spectroscopy. The following structure of the trisaccharide repeating-unit of the polysaccharide was established:
Kocharova
Nina A
NA
ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991, GSP-1, Moscow, Russia.
Zatonsky
George V
GV
Bystrova
Olga V
OV
Ziolkowski
Andrzej
A
Wykrota
Marianna
M
Shashkov
Aleksander S
AS
Knirel
Yuriy A
YA
Rozalski
Antoni
A
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D009112:Muramic Acids / Q000737:chemistry* / Q000302:isolation & purification*; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry*; D009904:Optical Rotation; D011532:Providencia / Q000737:chemistry* / Q000145:classification
D009112:Muramic Acids; D019081:O Antigens; C031651:N-acetylmuramic acid