title: Impaired renal Na(+) retention in the sgk1-knockout mouse.
abstract: The serum- and glucocorticoid-regulated kinase (sgk1) is induced by mineralocorticoids and, in turn, upregulates heterologously expressed renal epithelial Na(+) channel (ENaC) activity in Xenopus oocytes. Accordingly, Sgk1 is considered to mediate the mineralocorticoid stimulation of renal ENaC activity and antinatriuresis. Here we show that at standard NaCl intake, renal water and electrolyte excretion is indistinguishable in sgk1-knockout (sgk1(-/-)) mice and wild-type (sgk1(+/+)) mice. In contrast, dietary NaCl restriction reveals an impaired ability of sgk1(-/-) mice to adequately decrease Na(+) excretion despite increases in plasma aldosterone levels and proximal-tubular Na(+) and fluid reabsorption, as well as decreases in blood pressure and glomerular filtration rate.
authors.affiliation: Zentrum für Molekulare Neurobiologie, University of Hamburg, Hamburg, Germany.
authors.forename: Peer
authors.initials: P
authors.lastname: Wulff
authors.forename: Volker
authors.initials: V
authors.lastname: Vallon
authors.forename: Dan Yang
authors.initials: DY
authors.lastname: Huang
authors.forename: Harald
authors.initials: H
authors.lastname: Völkl
authors.forename: Fang
authors.initials: F
authors.lastname: Yu
authors.forename: Kerstin
authors.initials: K
authors.lastname: Richter
authors.forename: Martina
authors.initials: M
authors.lastname: Jansen
authors.forename: Michaela
authors.initials: M
authors.lastname: Schlünz
authors.forename: Karin
authors.initials: K
authors.lastname: Klingel
authors.forename: Johannes
authors.initials: J
authors.lastname: Loffing
authors.forename: Gunther
authors.initials: G
authors.lastname: Kauselmann
authors.forename: Michael R
authors.initials: MR
authors.lastname: Bösl
authors.forename: Florian
authors.initials: F
authors.lastname: Lang
authors.forename: Dietmar
authors.initials: D
authors.lastname: Kuhl
mesh_terms: D000818:Animals; D053503:Epithelial Sodium Channels; D005260:Female; D017874:Immediate-Early Proteins; D007668:Kidney / Q000378:metabolism*; D007671:Kidney Concentrating Ability; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases / Q000502:physiology*; D012964:Sodium / Q000378:metabolism*; D015222:Sodium Channels / Q000032:analysis; D017673:Sodium Chloride, Dietary / Q000008:administration & dosage; D000099184:Serum-Glucocorticoid Regulated Kinases
chemical_list: D053503:Epithelial Sodium Channels; D017874:Immediate-Early Proteins; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases; D012964:Sodium; D015222:Sodium Channels; D017673:Sodium Chloride, Dietary; D000099184:Serum-Glucocorticoid Regulated Kinases