[Effects of cartilage-derived growth factor on cultured rabbit chondrocytes].
OBJECTIVE
To investigate the effect of cartilage-derived growth factor (CDGF) on cultured rabbit chondrocytes, and the relation between CDGF, insulin-like growth factor-I (IGF-I), and proliferation and metabolism of chondrocytes.


METHODS
CDGF was extracted from chicken xiphoid in our laboratory. Chondrocytes were isolated from 3-week-old New Zealand male rabbits, and cultured in Ham's F-12 medium containing 10% fetal calf serum. The chondrocyts of second generation were treated with CDGF and/or IGF-I of different concentrations. With chloramine T method and MTT method, we compared the content of hydroxyproline in Ham's F-12 medium and observed proliferation and energy synthesis of chondrocytes.


RESULTS
CDGF and IGF-I both stimulated the proliferation and synthesis of hydroxyproline of chondrocytes dose-dependently. The optimal concentration of CDGF was 16 ng/ml and 32 ng/ml respectively, and that of IGF-I was 30 ng/ml. There was obvious synergic effect between CDGF and IGF-I.


CONCLUSIONS
CDGF can stimulate the proliferation and collagen synthesis of chondrocytes and has synergistic effect with IGF-I.
Ma
Jinfeng
J
Department of Orthopaedics, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, China.
Chen
Xiaoliang
X
Wang
Yingzhen
Y
Wang
Jixian
J
Huang
Hongshi
H
D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D019902:Chondrocytes / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006909:Hydroxyproline / Q000096:biosynthesis; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D008297:Male; D011506:Proteins / Q000494:pharmacology*; D011817:Rabbits
D011506:Proteins; D007334:Insulin-Like Growth Factor I; D006909:Hydroxyproline