Association of GYS1 and beta(3)-AR gene with postprandial hyperglycemia and serum uric acid in type 2 diabetes mellitus.
OBJECTIVE
To determine the relationships of Met416Val and XbaI polymorphism of muscle glycogen synthase (GYS1) gene and Trg64Arg variant of the beta(3)-adrenergic-receptor (beta(3)-AR) gene with type 2 diabetes mellitus (DM) and its intermediate phenotypes in the Chinese population.


METHODS
Polymerase chain reaction-oligonucleotide ligation assay and restriction fragment length polymorphism assay were used to evaluate the GYS1 and beta(3)-AR gene polymorphisms in 102 pairs of case-control Chinese spouses.


RESULTS
Subjects with Met416Val variant had a significantly higher 2-hour post-glucose level than subjects without this variant had in diabetic group (P = 0.032). The Met416Val polymorphism of GYS1 gene was not significantly associated with the risk of type 2 DM (adjusted OR = 1.67; 95% CI: 0.73 - 3.81, P = 0.223). Subjects with Trp64Arg variant had a significantly higher serum uric acid level than subjects without this variant had in diabetic group (P = 0.034). The combination of BMI and Arg64 allele carrier of the beta(3)-AR gene increased the diabetic risk over four-fold (adjusted OR = 4.00; 95% CI: 1.53 - 10.45, P = 0.005).


CONCLUSIONS
In the Chinese population, Met416Val polymorphism is identified in a subgroup of diabetic subjects with high 2-hour post-glucose. It will explain why some diabetic patients appear to be genetically predisposed to developing high postpradial glucose level. The presence of the Arg64 allele in the beta(3)-AR gene may predispose patients to higher serum uric acid level.
Wang
Guoying
G
Department of Endocrinology, The Third Hospital of Beijing University, China.
Li
Qiongfang
Q
Niu
Tianhua
T
Chen
Changzhong
C
Xu
Xiping
X
D000328:Adult; D000368:Aged; D000483:Alleles; D015992:Body Mass Index; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics*; D006006:Glycogen Synthase / Q000235:genetics*; D006801:Humans; D006943:Hyperglycemia / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D019518:Postprandial Period / Q000502:physiology*; D022702:Receptors, Adrenergic, beta-3 / Q000235:genetics*; D014527:Uric Acid / Q000097:blood*
D022702:Receptors, Adrenergic, beta-3; D014527:Uric Acid; D006006:Glycogen Synthase