title: Protective effects of polypeptide from Chlamys farreri on Hela cells damaged by ultraviolet A.
abstract: OBJECTIVE
  To study the protective effect of polypeptide isolated from Chlamys farreri (PCF) on Hela cells damaged by ultraviolet A (UVA) in vitro.
  
  
  METHODS
  Cell proliferation was determined by MTT method; intra-cellular free calcium [Ca2+]i and rates of apoptosis and death were measured by flow cytometry (FCM).
  
  
  RESULTS
  PCF (0.5 %-2 %) enhanced the activities of glutathione peroxidase (GSH-px), superoxide dismutase (SOD), and catalase (CAT), and stimulated cell proliferation. The concentration of [Ca2+]i was increased while the amounts of MDA and the rates of apoptosis and death of the cells were decreased. The differences between the PCF groups and control group were significant (P<0.05, P<0.01).
  
  
  CONCLUSIONS
  PCF protected Hela cells against damage by UVA via its anti-oxidative mechanisms.
authors.affiliation: Hospital of Medical College, Qingdao University, Qingdao 266003, China.
authors.forename: Ru-Yong
authors.initials: RY
authors.lastname: Yao
authors.forename: Chun-Bo
authors.initials: CB
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002118:Calcium / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D010125:Ostreidae / Q000737:chemistry*; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D008419:Materia Medica; D010455:Peptides; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D002118:Calcium