Mucosal immunization with live recombinant bovine respiratory syncytial virus (BRSV) and recombinant BRSV lacking the envelope glycoprotein G protects against challenge with wild-type BRSV.
Recombinant bovine respiratory syncytial virus (rBRSV) and an rBRSV deletion mutant lacking the G gene (rBRSVDeltaG) were characterized in calves with respect to replication competence, attenuation, and protective efficacy as live-attenuated BRSV vaccines. Both recombinant viruses were safe and induced protection against a BRSV challenge infection. rBRSV replicated efficiently in the upper respiratory tract. Intranasal immunization with rBRSVDeltaG led to infection but not to mucosal virus replication. Neutralizing antibodies were induced by rBRSV and rBRSVDeltaG. Thus, the BRSV attachment glycoprotein G seems to be dispensable in vaccinating calves against BRSV.
Schmidt
Ulrike
U
Institute of Molecular Biology, Federal Research Centre for Virus Diseases of Animals, D-17498 Insel Riems, Germany.
Beyer
Jörg
J
Polster
Ulf
U
Gershwin
Laurel J
LJ
Buchholz
Ursula J
UJ
D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D017353:Gene Deletion; D005814:Genes, Viral; D018928:Immunity, Mucosal; D007114:Immunization / Q000662:veterinary; D008168:Lung / Q000473:pathology; D009500:Neutralization Tests; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D018112:Respiratory Syncytial Virus, Bovine / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity / Q000502:physiology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000494:pharmacology; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000494:pharmacology; D014779:Virus Replication
D000914:Antibodies, Viral; D000956:Antigens, Viral; C066028:G protein, Bovine respiratory syncytial virus; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines