Primary cultures from the marine sponge Xestospongia muta (Petrosiidae, Haplosclerida).
In the context of the investigations on the origin and in vitro production of bioactive compounds, primary cultures were developed from ectosomal and choanosomal cell suspensions from the sponge Xestospongia muta. Dissociated cells aggregated and reorganized into a striking reticulated network of cells, typical for X. muta. Moreover, in some cultures an isotropic reticulation of small spicules, very similar to that found in the ectosome of adult sponges, was observed. Phytohaemagglutinin promoted aggregation and the reorganization of the cells. HPLC analyses revealed that straight-chain acetylenic compounds were recovered from short-term cultures and that they were synthesized during culture. Heterotrophic bacteria were assumed to be involved in the process. Together our results established that X. muta would be an excellent experimental model to study, in laboratory conditions, the differentiation of the skeleton and the in vitro biosynthesis of straight-chain acetylenic compounds.
Richelle-Maurer
Evelyn
E
Laboratory of Cellular Physiology, IBMM, CP 300, Université Libre de Bruxelles, 50 av. F.D. Roosevelt, B-1050 Brussels, Belgium. emaurer@ulb.ac.be
Gomez
Raquel
R
Braekman
Jean-Claude
JC
Van de Vyver
Gisèle
G
Van Soest
Rob W M
RW
Devijver
Christine
C
D000480:Alkynes; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017691:Caribbean Region; D002449:Cell Aggregation / Q000187:drug effects / Q000502:physiology; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002457:Cell Extracts / Q000737:chemistry; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D046508:Culture Techniques / Q000379:methods*; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000737:chemistry; D005431:Florida; D008386:Marine Biology / Q000379:methods; D009024:Morphogenesis / Q000187:drug effects / Q000502:physiology; D011161:Porifera / Q000737:chemistry / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism; D011786:Quality Control; D012623:Seawater; D013696:Temperature
D000480:Alkynes; D000900:Anti-Bacterial Agents; D002457:Cell Extracts; D005231:Fatty Acids, Unsaturated; C031203:2-hexadecynoic acid