Oxytocin induces PGE2 release from bovine cervical mucosa in vivo.
Oxytocin receptor (OTR) concentrations in bovine cervical mucosa rise steeply a few days before estrus to high concentrations and fall rapidly after estrus. To study the physiological role of these OTR, the effect of OT on the release of PGE, from the cervical mucosa of periestrous cows in vivo was determined by inserting bags made of dialysis tubing containing isooncotic saline solution in the endocervix for two 2-h periods, a fresh bag for each period. During the first period no treatment was given, during the second period OT (100 IU) or saline was injected i.m. PGE2 content in the second bag was significantly greater in OT-treated cows than in saline-treated cows. In a second experiment cervical resistance to stretch, achieved by distention of a balloon inside the cervical canal, was measured in periestrous cows before and 10 h after i.m. injection of OT, or endocervical application of 2.5mg PGE1 in a jelly, or the inactive jelly. A significant reduction in the resistance was achieved with both OT and PGE1; in the doses given the effect of PGE1 was longer lasting than that of OT.
Fuchs
A R
AR
Department of Obstetrics and Gynecology, Cornell University Medical College, New York, NY 10128, USA. annariitta@aol.com
Graddy
L G
LG
Kowalski
A A
AA
Fields
M J
MJ
D000818:Animals; D002417:Cattle; D002582:Cervix Mucus / Q000737:chemistry / Q000187:drug effects; D002584:Cervix Uteri / Q000187:drug effects / Q000378:metabolism*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology; D015232:Dinoprostone / Q000378:metabolism*; D004971:Estrus; D005260:Female; D016595:Misoprostol / Q000494:pharmacology; D009092:Mucous Membrane / Q000378:metabolism; D010121:Oxytocin / Q000494:pharmacology*; D018045:Receptors, Oxytocin / Q000502:physiology
D018045:Receptors, Oxytocin; D016595:Misoprostol; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D010121:Oxytocin; D015237:Dinoprost; D015232:Dinoprostone