title: A hydrogel prepared by in situ cross-linking of a thiol-containing poly(ethylene glycol)-based copolymer: a new biomaterial for protein drug delivery.
abstract: A new poly(ethylene glycol)-based copolymer containing multiple thiol (-SH) groups was cross-linked in situ to form a polymer hydrogel under mild conditions. No organic solvent, elevated temperature, or harsh pH is required in the formulation or patient administration processes, making it particularly useful for delivery of fragile therapeutics, such as proteins. The in vitro release of fluorescein-labeled bovine serum albumin and the in vivo release of the model proteins, erythropoietin, RANTES and three PEG-conjugated RANTES derivatives showed sustained release for 2-4 weeks and demonstrated prolonged biological activity of the released proteins in animals.
authors.affiliation: Center for Advanced Biotechnology and Medicine, Rutgers University, 679 Hoes Lane, Piscataway, NJ 08854, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Qiu
authors.forename: Simon
authors.initials: S
authors.lastname: Stefanos
authors.forename: Jianglin
authors.initials: J
authors.lastname: Ma
authors.forename: Anita
authors.initials: A
authors.lastname: Lalloo
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Perry
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Leibowitz
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Sinko
authors.forename: Stanley
authors.initials: S
authors.lastname: Stein
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D002417:Cattle; D018946:Chemokine CCL5 / Q000008:administration & dosage; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D004921:Erythropoietin / Q000008:administration & dosage; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry; D066298:In Vitro Techniques; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry; D011506:Proteins / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins; D012449:Safety; D012710:Serum Albumin, Bovine / Q000008:administration & dosage
chemical_list: D001672:Biocompatible Materials; D018946:Chemokine CCL5; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D020100:Hydrogels; D011506:Proteins; D011994:Recombinant Proteins; D004921:Erythropoietin; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols