title: Hispin, a novel ribosome inactivating protein with antifungal activity from hairy melon seeds.
abstract: A ribosome inactivating protein demonstrating a molecular mass of 21 kDa and a novel N-terminal sequence was isolated from seeds of the hairy melon. The purification procedure involved affinity chromatography on Affi-gel blue gel and ion exchange chromatography on CM-Sepharose and Mono S. The protein designated hispin inhibited translation in the cell-free rabbit reticulocyte lysate system with an IC50 of 165 pM and exhibited N-glycosidase activity. Antifungal activity was also observed.
authors.affiliation: Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China. biochemistry@cuhk.edu.hk
authors.forename: T B
authors.initials: TB
authors.lastname: Ng
authors.forename: A
authors.initials: A
authors.lastname: Parkash
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002846:Chromatography, Affinity; D028462:Cucumis / Q000196:embryology*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012270:Ribosomes / Q000187:drug effects*; D012639:Seeds / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents; D010940:Plant Proteins