Effects of novel human chemokine-like factor 1 (CKLF1) on bone marrow hematopoietic stem cell/progenitor cell in vitro.
OBJECTIVE
To explore the effects of a novel human chemokine-like factor 1 (CKLF1) on stem cell/progenitor cells.


METHODS
Human bone marrow mononuclear cells were separated by Ficoll (1.077 g/ml), and cultured in suspension and semisolid colony culture. The effects of CKLF1 on CFU-GM and CFU-Mix were observed.


RESULTS
The number of CFU-GM was significantly increased in 10 groups by addition of CKLF1 plasmid supernatant. The mean value was 234.81 +/- 98.71/1 x 10(5) cells, 2.42 fold of control group (P < 0.05). The mean value of CFU-Mix in groups of negative control, CKLF1, PHA, GM-CSF and G-CSF were 82.00 +/- 20.25, 105.76 +/- 36.70, 146.63 +/- 27.09, 143.33 +/- 60.23 per 1 x 10(5) cells, respectively, no statistical differences were seen between control and CKLF1 groups. The CD(34)(+) cells were detected by FACS. The average percentage in the groups of normal control, CKLF1, PHA and GM-CSF were (0.75 +/- 0.62)%, (1.32 +/- 0.87)%, (0.15 +/- 0.02)%, and (0.29 +/- 0.23)%, respectively. Compared with control, no significant differences were seen between each group (P > 0.05).


CONCLUSIONS
Novel chemokine-like factor 1 can increase the proliferation of CFU-GM, which indicated that CKLF1 could increase the proliferation of human bone marrow hematopoietic progenitor cells and colony formation.
Ke
Xiaoyan
X
Department of Hematology, The Third Hospital of Beijing University, Beijing 100083, China.
Jia
Liping
L
Jing
Hongmei
H
Liu
Yan
Y
Zhang
Yingmei
Y
Di
Chunhui
C
D000328:Adult; D002478:Cells, Cultured; D018925:Chemokines / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D062792:MARVEL Domain-Containing Proteins
C432676:CKLF protein, human; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins