title: Circadian rhythm of gene expression of myocardial contractile protein, left ventricular pressure and contractility.
abstract: Objective. A number of cardiovascular variables exhibit a circadian rhythm. Whether myocardial contractile response and gene expression of the contractile protein also show changes with a similar period was here investigated. Method. Circadian variabilities in the left ventricular developed pressure (LVP) and contractility (LV dp/dt max) were measured in 24 Sprague-Dawley rats by directly left ventricular catheterizing and compared with changes in the gene expression of alpha-myosin heavy chain (alpha-MHC) in myocytes obtained from the same animals by dot blotting analysis. Results. A circadian rhythm was seen in the variability of LVP (P<0.001), LV dp/dt max (P<0.001) and the biochemically measured expression of the alpha-MHC gene (P<0.01). As compared to the amplitude of the rhythm in alpha-MHC gene expression, the amplitude of the contractility rhythm was large (P<0.01) and the circadian amplitude of the LVP (P<0.001) was the largest, representing perhaps a composite of intracardiac plus any extracardiac contributions. Conclusion. One of factors determining the circadian rhythm of myocardial contractile function is alpha-MHC gene expression level.
authors.affiliation: Biomedical Engineering Department, West China University of Medical Sciences, Chengdu, P.R. China.
authors.forename: Z R
authors.initials: ZR
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: C M
authors.initials: CM
authors.lastname: Wan
authors.forename: G
authors.initials: G
authors.lastname: Cornelissen
authors.forename: I
authors.initials: I
authors.lastname: Anand
authors.forename: F
authors.initials: F
authors.lastname: Halberg
mesh_terms: D000818:Animals; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology*; D003285:Contractile Proteins / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006339:Heart Rate / Q000235:genetics / Q000502:physiology; D008297:Male; D009200:Myocardial Contraction / Q000235:genetics / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016277:Ventricular Function, Left / Q000235:genetics / Q000502:physiology*; D017725:Ventricular Pressure / Q000235:genetics / Q000502:physiology*
chemical_list: D003285:Contractile Proteins; D018995:Myosin Heavy Chains