The aberrant expression of cytosolic carbonic anhydrase and its clinical significance in human non-small cell lung cancer.
This study was designed to elucidate the possible relationship between the expression of cytosolic carbonic anhydrase (CA) and non-small cell lung cancer (NSCLC). The activity and protein expression patterns of carbonic anhydrase I (CAI) and II (CAII) of 70 NSCLC patients were analyzed by CA activity analysis, immunoblotting and immunohistochemical staining. The results showed that the CA activity and protein expression were significantly decreased in both squamous cell carcinoma (SCC) and adenocarcinoma (AD) (P<0.001 and P<0.001). From our study, it was suggested that the reduction of CAI and CAII in both SCC and AD patients may promote tumor cell motility and contribute to tumor growth and metastasis.
Chiang
Whei Ling
WL
School of Medical Technology, Chung Shan Medical University, No 110, Section 1, Chien Kuo N. Road, Taichung 402, Taiwan.
Chu
Shu Chen
SC
Yang
Shyh Sheng
SS
Li
Mu Chun
MC
Lai
Jyh Chang
JC
Yang
Shun Fa
SF
Chiou
Hui Ling
HL
Hsieh
Yih Shou
YS
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D024401:Carbonic Anhydrase I / Q000378:metabolism*; D024402:Carbonic Anhydrase II / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology* / Q000601:surgery; D003600:Cytosol; D015536:Down-Regulation; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000201:enzymology* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D011379:Prognosis
D014408:Biomarkers, Tumor; D024401:Carbonic Anhydrase I; D024402:Carbonic Anhydrase II