A first-generation EST RH comparative map of the porcine and human genome.
We have constructed a first-generation EST radiation hybrid comparative map of the porcine genome by assigning 1,058 markers to the IMpRH(7000) panel. Chromosomal localization was determined with a 2pt LOD of 4.8 for 984 markers, using the IMpRH mapping tool. Annotated ESTs represent 46.2% or 489 of the markers. Marker distribution was not stochastic and ranged from 0.41 for SSC8 to 1.77 for SSC12, respectively. Two hundred fifty-one markers assigned to the physical map of the pig did not find a homologous sequence in V22 of the human genome assembly, indicative of gaps in the assembled human genome sequence. The comparative porcine/human map covers 3,290 MB, or 98.3% of the presumed size of the human genome. However, 60 breakpoints were identified between chromosomes, as well as 90 micro-rearrangements within synteny groups. Six porcine chromosomes-SSC2, 5, 6, 7, 12, and 14-correspond to the three gene-richest human chromosomes, HSA17, 19, and 22, and show above average marker density. Porcine Chrs 1, 8, 11, and X display a low DNA/marker ratio and correspond to the 'genome deserts' on HSA 18, 4, 13, and X.
Rink
Anette
A
Department of Animal Biotechnology, College of Agriculture, Biotechnology and Natural Resources, University of Nevada, Reno, Nevada 89557, USA. arink@cabnr.unr.edu
Santschi
Elizabeth M
EM
Eyer
Katie M
KM
Roelofs
Benjamin
B
Hess
Markus
M
Godfrey
Myra
M
Karajusuf
Elif K
EK
Yerle
Martine
M
Milan
Denis
D
Beattie
Craig W
CW
D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D006822:Hybrid Cells; D021101:Radiation Hybrid Mapping / Q000379:methods / Q000662:veterinary*; D013552:Swine / Q000235:genetics*; D026801:Synteny
D005819:Genetic Markers