title: Subunit communications crucial for the functional integrity of the yeast RNA polymerase II elongator (gamma-toxin target (TOT)) complex.
abstract: In response to the Kluyveromyces lactis zymocin, the gamma-toxin target (TOT) function of the Saccharomyces cerevisiae RNA polymerase II (pol II) Elongator complex prevents sensitive strains from cell cycle progression. Studying Elongator subunit communications, Tot1p (Elp1p), the yeast homologue of human IKK-associated protein, was found to be essentially involved in maintaining the structural integrity of Elongator. Thus, the ability of Tot2p (Elp2p) to interact with the HAT subunit Tot3p (Elp3p) of Elongator and with subunit Tot5p (Elp5p) is dependent on Tot1p (Elp1p). Also, the association of core-Elongator (Tot1-3p/Elp1-3p) with HAP (Elp4-6p/Tot5-7p), the second three-subunit subcomplex of Elongator, was found to be sensitive to loss of TOT1 (ELP1) gene function. Structural integrity of the HAP complex itself requires the ELP4/TOT7, ELP5/TOT5, and ELP6/TOT6 genes, and elp6Delta/tot6Delta as well as elp4Delta/tot7Delta cells can no longer promote interaction between Tot5p (Elp5p) and Tot2p (Elp2p). The association between Elongator and Tot4p (Kti12p), a factor that may modulate the TOT activity of Elongator, requires Tot1-3p (Elp1-3p) and Tot5p (Elp5p), indicating that this contact requires a preassembled holo-Elongator complex. Tot4p also binds pol II hyperphosphorylated at its C-terminal domain Ser(5) raising the possibility that Tot4p bridges the contact between Elongator and pol II.
authors.affiliation: Institut für Genetik, Biologicum, Martin-Luther-Universität Halle-Wittenberg, Weinbergweg 10, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Frohloff
authors.forename: Daniel
authors.initials: D
authors.lastname: Jablonowski
authors.forename: Lars
authors.initials: L
authors.lastname: Fichtner
authors.forename: Raffael
authors.initials: R
authors.lastname: Schaffrath
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry / Q000378:metabolism*; D051548:Histone Acetyltransferases; D051550:I-kappa B Kinase; D055646:Killer Factors, Yeast; D009183:Mycotoxins / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D021122:Protein Subunits; D012319:RNA Polymerase II / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D055646:Killer Factors, Yeast; D009183:Mycotoxins; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; C058975:zymocin; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; D017346:Protein Serine-Threonine Kinases; D051550:I-kappa B Kinase; D012319:RNA Polymerase II