Evaluation of an enzyme-linked immunosorbent assay (ELISA) with affinity-purified Em18 and an ELISA with recombinant Em18 for differential diagnosis of alveolar echinococcosis: results of a blind test.
Alveolar echinococcosis (AE) is the most potentially lethal parasitic zoonosis of the nontropical areas in the northern hemisphere, where cystic echinococcosis (CE) is also endemic. Both AE and CE are highly endemic in China, and both serologic detection of echinococcosis, either AE or CE, and differentiation of AE from CE are crucial problems. Evaluation of Western blot analysis (WB) and enzyme-linked immunosorbent assay (ELISA) for the Em18 antigen, using affinity-purified and recombinant Em18, was carried out "blindly" using 60 human sera from patients diagnosed in France. The results were compared with those obtained using a commercially available Echinococcus WB immunoglobulin G (IgG) kit developed in France. The Em18 WB and Echinococcus WB IgG showed very similar results for detection of AE. Both affinity-purified Em18 or a recombinant Em18 WB and Echinococcus WB IgG seem useful for identification of AE, and the latter seems appropriate for both AE and CE, whereas affinity-purified Em18 ELISA and the newly developed recombinant Em18 ELISA appear to be suitable for detection of AE, especially for epidemiological surveys.
Ito
Akira
A
Department of Parasitology. Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa, Japan. akiraito@asahikawa-med.ac.jp
Xiao
Ning
N
Liance
Martine
M
Sato
Marcello O
MO
Sako
Yasuhito
Y
Mamuti
Wulamu
W
Ishikawa
Yuji
Y
Nakao
Minoru
M
Yamasaki
Hiroshi
H
Nakaya
Kazuhiro
K
Bardonnet
Karine
K
Bresson-Hadni
Solange
S
Vuitton
Dominique A
DA
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D015415:Biomarkers; D002846:Chromatography, Affinity; D003937:Diagnosis, Differential; D004444:Echinococcosis, Hepatic / Q000175:diagnosis* / Q000469:parasitology; D004446:Echinococcus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011994:Recombinant Proteins / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015415:Biomarkers; C117889:Em18 antigen; D011994:Recombinant Proteins