Determination of melanotan-II in rat plasma by liquid chromatography/tandem mass spectrometry: determination of pharmacokinetic parameters in rat following intravenous administration.
A method is described for the determination of melanotan-II (MT-II), a synthetic cyclic heptapeptide analog of alpha-melanocyte-stimulating hormone (alpha-MSH), in rat plasma by liquid chromatography/tandem mass spectrometry (LC/MS/MS). The analyte is recovered from plasma by solid-phase extraction (SPE) and subsequently analyzed by LC/MS/MS. A SPE procedure using OASIS 96-well plates is used for extraction of MT-II from rat plasma. Using the described approach a limit of quantitation of 5 ng/mL was achieved in rat plasma. This level of sensitivity allowed the determination of pharmacokinetic parameters, following intravenous administration of MT-II in rat.
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Merck Research Laboratories, Rahway, NJ 07065, USA.
Shen
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Tamvakopoulos
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D000818:Animals; D001682:Biological Availability; D002853:Chromatography, Liquid / Q000379:methods*; D007262:Infusions, Intravenous; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D000521:alpha-MSH / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics*
D010456:Peptides, Cyclic; C079282:melanotan-II; D000521:alpha-MSH