Cysteamine pre-treatment reduces pentylenetetrazol-induced plasticity and epileptiform discharge in the CA1 region of rat hippocampal slices.
The effects of prior treatment of cysteamine, a somatostatin inhibitor, on pentylenetetrazol (PTZ) induced epileptic and plastic changes in CA1 excitability were examined. Population spikes were evoked by activation of Schaffer collaterals with a range of stimulation intensities. Changes in the population spike and epileptiform amplitudes were used as indices to quantify the effects of PTZ exposure in the control and cysteamine pre-treated slices. Cysteamine pre-treatment decreased baseline CA1 population spike amplitude following high intensity stimulation of Schaffer collaterals. Following PTZ application directly to the slices, cysteamine diminished the increased population spike and epileptiform amplitudes which were normally observed following collateral stimulation. Magnesium-free medium induced epileptiform activity was also significantly reduced with cysteamine pre-treatment. It is concluded that somatostatin may be involved in PTZ-induced epileptic and plastic changes in CA1 excitability.
Rostampour
Mohammad
M
Department of Physiology, School of Medical Sciences, Tarbiat Modarres University, PO Box 14155-111, Tehran, Iran.
Fathollahi
Yaghoub
Y
Semnanian
Saeed
S
Hajizadeh
Sohrab
S
Mirnajafizadeh
Javad
J
Shafizadeh
Mahshied
M
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D003543:Cysteamine / Q000494:pharmacology* / Q000627:therapeutic use; D004827:Epilepsy / Q000139:chemically induced* / Q000188:drug therapy / Q000503:physiopathology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D009473:Neuronal Plasticity / Q000187:drug effects* / Q000502:physiology; D010433:Pentylenetetrazole / Q000009:adverse effects*; D051381:Rats
D003543:Cysteamine; D010433:Pentylenetetrazole