[Promotion of post BMT hematopoiesis reconstitution by cotransplantation of IL-3 transfected marrow stromal cells in mice].
OBJECTIVE
To explore whether bone marrow stromal cell line QXMSC1 (H-2(d)) engineered to secrete IL-3 (QXMSC1 IL-3) can improve the hematopoiesis post-allogeneic bone marrow transplantation (BMT) in mice.


METHODS
The stromal cell line QXMSC1 IL-3 was established by transfecting QXMSC1 (H-2(d)) cell with a recombined retrovirus vector PL3SN containing mice IL-3 gene cDNA. Lethally irradiated mice C57BL/6 (H-2(b)) were transplanted with T cell depleted allogeneic bone marrow (BALB/c, H-2(d), 1 x 10(7)/mice) and QXMSC1 IL-3 cells (5 x 10(5)/mice). The numbers of RBC and WBC in peripheral blood were counted 20 and 40 days after bone marrow transplantation. The marrow nucleated cells, CFU-S, CFU-GM, CFU-E and CFU-GEMM yields were measured in recipient mice.


RESULTS
QXMSC1 IL-3 cells could stably secrete IL-3 and increase the peripheral RBC and WBC counts as well as the number of marrow nucleated cells and CFU-GM, CFU-E, CFU-GEMM yields.


CONCLUSIONS
Cotransplantation of QXMSC1 IL-3 cells with T cell depleted marrow grafts improve hematopoiesis post allogeneic BMT in mice.
Jiang
Jiyang
J
Department of Immunology, Health Science Center, Peking University, Beijing 100083, China.
Zhang
Jingmei
J
Hao
Jie
J
Xie
Shusheng
S
D000818:Animals; D016026:Bone Marrow Transplantation; D004912:Erythrocytes / Q000166:cytology; D005260:Female; D006410:Hematopoiesis / Q000235:genetics / Q000502:physiology*; D007377:Interleukin-3 / Q000235:genetics*; D007962:Leukocytes / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D017154:Stromal Cells / Q000378:metabolism / Q000637:transplantation*; D013997:Time Factors; D014162:Transfection
D007377:Interleukin-3