Rapid spectrophotometric method for serum glutathione S-transferases activity.
BACKGROUND
Glutathione S-transferases (GST) are a family of polymorphic enzymes responsible for the metabolism of a broad range of xenobiotics including carcinogens and endogenous compounds.


METHODS
The aim of this study was to develop a fully automated spectrophotometric method to determine serum GST activity.


RESULTS
The method had a detection limit of 7 U/l. The within- and between-run repeatability reached 2% and 3%, respectively. The reproducibility tested over a 6-month period was 7%. Serum GST activity is stable for at least 1 week at +37, +4 and -20 degrees C, 1 month at -20 degrees C and 6 months at -80 and -196 degrees C. The GST activities were determined on 374 supposedly healthy subjects coming to the Center of Preventive Medicine for a health screening. The sample population comprised 178 males and 196 females 4-80 years of age. The mean value of the serum GST activity calculated for this healthy population was 28.2+/-3.0 U/l without gender difference (range from 20.0 to 38.0 U/l). The global interindividual variability reached 11%.


CONCLUSIONS
This report describes a spectrophotometric assay for monitoring the serum GST activities in human. This method will be useful in examining the relationships between serum GST activities and the GST polymorphisms, especially pi-GST.
Habdous
Mohammed
M
Laboratoire du Centre de Médecine Préventive, Unité INSERM U525, BP 7, 2 Avenue du Doyen Jacques Parisot, Vandoeuvre-les-Nancy 54501, France.
Vincent-Viry
Monique
M
Visvikis
Sophie
S
Siest
Gérard
G
D000082:Acetaminophen / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D004137:Dinitrochlorobenzene / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability; D004976:Ethacrynic Acid / Q000494:pharmacology; D000431:Ethanol / Q000737:chemistry; D005260:Female; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000097:blood* / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000097:blood* / Q000737:chemistry; D008107:Liver Diseases / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*; D014227:Triazines / Q000494:pharmacology
D004137:Dinitrochlorobenzene; D004791:Enzyme Inhibitors; D007527:Isoenzymes; D014227:Triazines; D000082:Acetaminophen; D000431:Ethanol; C012842:Cibacron Blue F 3GA; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; C119995:glutathione S-transferase alpha; D004976:Ethacrynic Acid