Anti-Vel reactivity diminished by adsorption with rabbit RBC stroma.
BACKGROUND
An anti-Vel, nearly missed in antibody identification studies, and the effect of a commercially available rabbit RBC stroma (RESt, Immucor) adsorptions on eight anti-Vel sera are reported. Anti-Vel is an antibody to an antigen of high prevalence.


METHODS
A 48-year-old woman with chronic vaginal bleeding presented with a Hct of 14.7 percent. The transfusion service was not informed of her history of anti-Vel when she was transferred from another institution. Studies performed on an emergency request for transfusion were interpreted as a cold autoantibody as adsorption with a commercial source of RESt eliminated the reactivity. Stored anti-Vel sera were tested by titration studies before and after adsorption with commercial RESt.


RESULTS
Serum from the index case did not react after adsorption with RESt at the transfusion service. Studies with the stored anti-Vel indicated antibody adsorption with four of four samples at immediate spin (IS) and room temperature (RT) phases, four of eight samples at 37 degrees C in albumin (ALB) phase, and four of eight samples at ALB-IgG-AGT phase. Variations in antibody reactivity were observed in the samples tested, but RESt adsorption diminished antibody reactivity in most samples. All eight stored sera demonstrated some reactivity in at least one phase after adsorption with RESt.


CONCLUSIONS
Anti-Vel was completely or partially adsorbed by RESt. Caution should be used when interpreting cold agglutinins with this method. The manufacturer warns that uncommon alloantibodies may be adsorbed.
Mechanic
Steven A
SA
Department of Pathology and Laboratory Medicine, Temple University Hospital, Philadelphia, Pennsylvania, USA.
Maurer
Joan L
JL
Igoe
Mark J
MJ
Kavitsky
Donna M
DM
Nance
Sandra T
ST
D000017:ABO Blood-Group System / Q000097:blood; D000327:Adsorption; D000373:Agglutinins / Q000097:blood*; D000740:Anemia / Q000209:etiology / Q000628:therapy; D000818:Animals; D001789:Blood Group Antigens / Q000097:blood / Q000276:immunology; D001787:Blood Group Incompatibility / Q000097:blood / Q000175:diagnosis*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D002240:Carbohydrate Sequence; D002554:Cerebrosides / Q000276:immunology; D002908:Chronic Disease; D015897:Comorbidity; D003429:Cross Reactions; D003449:Cryoglobulinemia / Q000175:diagnosis*; D003450:Cryoglobulins; D003951:Diagnostic Errors; D000939:Epitopes / Q000276:immunology; D004912:Erythrocytes / Q000737:chemistry*; D005188:False Negative Reactions; D005260:Female; D006386:Hemagglutination Tests; D006801:Humans; D007114:Immunization; D007202:Indicators and Reagents; D007518:Isoantibodies / Q000097:blood* / Q000276:immunology; D007889:Leiomyoma / Q000150:complications; D008875:Middle Aged; D008969:Molecular Sequence Data; D011356:Product Labeling; D011817:Rabbits; D012015:Reference Standards; D013696:Temperature; D019851:Thrombophilia / Q000150:complications; D065227:Transfusion Reaction; D014592:Uterine Hemorrhage / Q000209:etiology; D014594:Uterine Neoplasms / Q000150:complications
D000017:ABO Blood-Group System; D000373:Agglutinins; D001789:Blood Group Antigens; D002554:Cerebrosides; D003450:Cryoglobulins; D000939:Epitopes; D007202:Indicators and Reagents; D007518:Isoantibodies; C029443:cold agglutinins; C041200:pentaglycosyl ceramide