Effects of perillyl alcohol in glial C6 cell line in vitro and anti-metastatic activity in chorioallantoic membrane model.
The search for new chemotherapeutic drugs has increased, especially for those that have a natural origin. The monoterpene perillyl alcohol (POH) has been shown to exert chemopreventive activity in mammary, liver, and lung tumor models. It has also been used to treat a variety of rodent cancers, including pancreatic and breast carcinomas. In vitro data suggest that it may be effective against neuroblastomas and leukemias. This work evaluates the effects of the treatment of murine glial C6 cells with perillyl alcohol. In vitro, our studies have indicated that POH inhibits proliferation of the C6 glial cell line. POH was logarithmically diluted in concentrations of 30% through 0.0003% and showed inhibition cell proliferation of 78.36% in concentration of 30%; 69.87% in concentration of 3%; and 67.04% in concentration of 0.03%. In addition, the anti-metastatic activity of POH against these cells was evaluated using chick embryos as an in vivo model. The experiments have shown anti-metastatic activity of POH when the C6 murine glial cells were exposed to a concentration of 0.3 to 0.003% POH for 2 h, prior to its inoculation in chick embryo chorioallantoic membrane model. This phenomenon shows the possible role of POH as an in vivo anti-metastatic drug.
Teruszkin Balassiano
Ilana
I
Instituto de Biofisica Carlos Chagas Filho, Laboratorio de Controle da Expressao Genica, Universidade Federal do Rio de Janeiro, Centro de Ciencias da Saude, Rio de Janeiro, Brazil.
Alves de Paulo
Sheila
S
Henriques Silva
Nathalie
N
Curié Cabral
Maulori
M
Gibaldi
Daniel
D
Bozza
Marcelo
M
Orlando da Fonseca
Clovis
C
Da Glória da Costa Carvalho
Maria
M
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001681:Biological Assay; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002642:Chick Embryo; D066298:In Vitro Techniques; D008954:Models, Biological; D039821:Monoterpenes / Q000494:pharmacology*; D009362:Neoplasm Metastasis / Q000188:drug therapy*; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
D000970:Antineoplastic Agents; D039821:Monoterpenes; D013778:Tetrazolium Salts; D013844:Thiazoles; C032208:perillyl alcohol; C022616:thiazolyl blue