title: Analysis of novel disease-related genes in bronchial asthma.
abstract: Bronchial asthma is a complex disease characterized by airway inflammation involving interleukin (IL)-4 and IL-13. We have applied microarray analyses to human bronchial epithelial cultures to probe for genes regulated by these cytokines and have identified a subset of disease-relevant genes by comparison with cDNA libraries derived from normal and asthmatic bronchial biopsies. Squamous cell carcinoma antigen-1 (SCCA1) and SCCA2, the cysteine and serine protease inhibitors, respectively, showed the highest expression by IL-4 and IL-13, and particularly, SCCA1 was significantly increased in the asthmatic cDNA library. STAT6 was shown to be involved in expression of SCCA1 and SCCA2 in vitro. Furthermore, serum levels of SCCA were also elevated in asthmatic patients. Taken together, it was supposed that SCCA may play some role in the pathogenesis of bronchia asthma, and measuring its serum level may be relevant for diagnosing or monitoring the status of bronchial asthma. In a complex disorder such as asthma, this combination of in vitro and in vivo genomic approaches is a powerful discriminatory method enabling identification of novel disease-related genes and their mechanisms of regulation.
authors.affiliation: Genox Research, Inc. Kawasaki, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Yuyama
authors.forename: Donna E
authors.initials: DE
authors.lastname: Davies
authors.forename: Mina
authors.initials: M
authors.lastname: Akaiwa
authors.forename: Keiko
authors.initials: K
authors.lastname: Matsui
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Hamasaki
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Suminami
authors.forename: Ning Lu
authors.initials: NL
authors.lastname: Yoshida
authors.forename: Miyako
authors.initials: M
authors.lastname: Maeda
authors.forename: Anita
authors.initials: A
authors.lastname: Pandit
authors.forename: James L
authors.initials: JL
authors.lastname: Lordan
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Kamogawa
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Arima
authors.forename: Fumio
authors.initials: F
authors.lastname: Nagumo
authors.forename: Mitsuhiko
authors.initials: M
authors.lastname: Sugimachi
authors.forename: Ann
authors.initials: A
authors.lastname: Berger
authors.forename: Ivan
authors.initials: I
authors.lastname: Richards
authors.forename: Steven L
authors.initials: SL
authors.lastname: Roberds
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Yamashita
authors.forename: Fumio
authors.initials: F
authors.lastname: Kishi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kato
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Arai
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohshima
authors.forename: Jutaro
authors.initials: J
authors.lastname: Tadano
authors.forename: Naotaka
authors.initials: N
authors.lastname: Hamasaki
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Miyatake
authors.forename: Yuji
authors.initials: Y
authors.lastname: Sugita
authors.forename: Stephen T
authors.initials: ST
authors.lastname: Holgate
authors.forename: Kenji
authors.initials: K
authors.lastname: Izuhara
mesh_terms: D000293:Adolescent; D000328:Adult; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000097:blood; D001249:Asthma / Q000235:genetics* / Q000378:metabolism; D001980:Bronchi / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007223:Infant; D018793:Interleukin-13 / Q000502:physiology; D015847:Interleukin-4 / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D020545:Respiratory Mucosa / Q000378:metabolism; D015843:Serpins
chemical_list: D000951:Antigens, Neoplasm; D018793:Interleukin-13; D015843:Serpins; C051072:squamous cell carcinoma-related antigen; D015847:Interleukin-4