[Molecular cloning of SRG2, a mouse testis spermatocyte apoptosis-related gene].
A novel mouse gene full-length cDNA sequence-SRG2 were identified (GenBank accession number AF395083), which was significantly changed in cryptorchidism, from a mouse testis cDNA library using a cDNA fragment (GenBank accession number BE644542) as an electronic probe. SRG2 was 1058 bp in length. The putative protein encoded by this gene was 295 amino acids with a theoretical molecular weight of 33 579 and isoelectric point of 9.64. The sequence shared no significant homology with any known protein in databases except TSARG2, with which its homology was 78%. RT-PCR showed that SRG2 was expressed significantly in testis.
Liu
Shang-Feng
SF
Human Reproductive Engineering Institute, Central South University Xiangya Med ical School, Changsha 410078, China. lgxdirector@sina.com
Li
Lu-Yun
LY
Fu
Jun-Jiang
JJ
Liu
Gang
G
Xing
Xiao-Wei
XW
Lu
Guang-Xiu
GX
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D015870:Gene Expression; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013090:Spermatocytes / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000378:metabolism*
D018076:DNA, Complementary; D011506:Proteins; C471347:SRG2 protein, mouse