The Nod factor-elicited annexin MtAnn1 is preferentially localised at the nuclear periphery in symbiotically activated root tissues of Medicago truncatula.
The Medicago truncatula MtAnn1 gene, encoding a putative annexin, is transcriptionally activated in root tissues in response to rhizobial Nod factors. To gain further insight into MtAnn1 function during the early stages of nodulation, we have examined in detail both spatio-temporal gene expression patterns and MtAnn1 activity and localisation in root tissues. Analysis of transgenic Medicago plants expressing a pMtAnn1-GUS fusion has revealed a novel pattern of transcription in both outer and inner cell layers of the root following either Nod factor-treatment or rhizobial inoculation. The highest gene expression levels were observed in the endodermis and outer cortex. These transgenic plants also revealed that MtAnn1 expression is associated with lateral root development and cell differentiation in the root apex independent of nodulation. By purifying recombinant MtAnn1 we were able to demonstrate that this plant annexin indeed possesses the calcium-dependent binding to acidic phospholipids typical of the annexin family. Antisera against recombinant MtAnn1 were then used to show that tissue-specific localisation of the MtAnn1 protein in Medicago roots matches the pMtAnn1-GUS expression pattern. Finally, both immunolabelling and in vivo studies using MtAnn1-GFP reporter fusions have revealed that MtAnn1 is cytosolic and in particular localises to the nuclear periphery in cortical cells activated during the early stages of nodulation. In the light of our findings, we discuss the possible role of this annexin in root tissues responding to symbiotic rhizobial signals.
De Carvalho-Niebel
Fernanda
F
Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, INRA-CNRS UMR 215, BP 27, 31326 Castanet-Tolosan Cedex, France. fniebel@toulouse.inra.fr
Timmers
Antonius C J
AC
Chabaud
Mireille
M
Defaux-Petras
Annie
A
Barker
David G
DG
D017305:Annexin A1 / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D049452:Green Fluorescent Proteins; D008070:Lipopolysaccharides / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D029902:Medicago / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D008856:Microscopy, Fluorescence; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D016962:Sinorhizobium meliloti / Q000254:growth & development; D013559:Symbiosis / Q000502:physiology
D017305:Annexin A1; D008070:Lipopolysaccharides; D008164:Luminescent Proteins; C092082:Nod factor IV, Rhizobium meliloti; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins