A homologue of human placental protein, PP11, and mouse T cell-specific protein, Tcl-30, in exocrine pancreas of a teleost (Paralichthys olivaceus).
We cloned a 1401-bp cDNA encoding a novel pancreatic protein (PPSB) with two cysteine-rich somatomedin B-like domains from Japanese flounder (Paralichthys olivaceus). PPSB is predicted to be composed of 385 amino acids, including a signal sequence. The peptide sequence shares high homology with human placental protein 11 (PP11) and mouse T-cell specific protein (Tcl-30), which both contain a single somatomedin B-like domain. PPSB shares 47% and 44% identity with PP11 and Tcl-30, respectively. The unique point of PPSB is that an additional, somatomedin B-like domain is tandemly inserted. Unlike PP11 and Tcl-30, PPSB mRNA is specifically expressed by the exocrine pancreatic acinar cells, together with trypsinogen. Since PP11 has serine protease activity, it is predicted that its teleost homologue, PPSB, may function as a pancreatic digestive enzyme.
Suzuki
Tohru
T
Metabolism Section, National Research Institute of Aquaculture, Fisheries Research Agency, Nansei, Mie 516-0193, Japan. suzukitr@fra.affrc.go.jp
Srivastava
Anand S
AS
Kurokawa
Tadahide
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004720:Endonucleases; D005432:Flounder / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010179:Pancreas / Q000737:chemistry*; D011257:Pregnancy Proteins / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017385:Sequence Homology; D013601:T-Lymphocytes / Q000737:chemistry*; D000086842:Uridylate-Specific Endoribonucleases
D018076:DNA, Complementary; D011257:Pregnancy Proteins; D012333:RNA, Messenger; C036270:placental protein 11; D004720:Endonucleases; C475860:Endou protein, mouse; D000086842:Uridylate-Specific Endoribonucleases