Inhibition of hepatitis delta virus RNA editing by short inhibitory RNA-mediated knockdown of ADAR1 but not ADAR2 expression.
Hepatitis delta virus (HDV) requires host RNA editing at the viral RNA amber/W site. Of the two host genes responsible for RNA editing via deamination of adenosines in double-stranded RNAs, short inhibitory RNA-mediated knockdown of host ADAR1 expression but not that of ADAR2 led to decreased HDV amber/W editing and virus production. Despite substantial sequence and structural variation among the amber/W sites of the three HDV genotypes, ADAR1a was primarily responsible for editing all three. We conclude that ADAR1 is primarily responsible for editing HDV RNA at the amber/W site during HDV infection.
Jayan
Geetha C
GC
Division of Molecular Virology and Immunology, Georgetown University Medical Center, Rockville, Maryland 20850, USA.
Casey
John L
JL
D000243:Adenosine Deaminase / Q000235:genetics* / Q000378:metabolism; D017398:Alternative Splicing; D001483:Base Sequence; D002460:Cell Line; D015870:Gene Expression; D005838:Genotype; D003698:Hepatitis Delta Virus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D006801:Humans; D017393:RNA Editing; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism*; D016601:RNA-Binding Proteins; D014162:Transfection; D014779:Virus Replication
D012367:RNA, Viral; D016601:RNA-Binding Proteins; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase