Cloning and characterization of an acidic cytolysin cDNA from sea anemone Sagartia rosea.
A full-length cDNA of cytolysin (Src I) was isolated from the tentacle of Sagartia rosea (a representative species in China) by reverse transcription polymerase chain reaction. The cDNA with an open reading frame of 648 bp encodes a precursor protein of 216 amino acids, which contains a prepropeptide of 38 amino acids including a signal peptide of 19 amino acids and a propart of 19 amino acids. Lys-Pro at C-terminus of propart is a cleavage site for proline-endopeptidase-like protease. The mature cytolysin has a molecular mass of 19.6 kDa and a pI value of 4.8. Src I is an acidic cytolysin found in sea anemone and shares 75% amino acid sequence similarity to equinatoxin II (Eqt II). The predicted secondary structure of the mature cytolysin comprises 15% alpha-helix, 45% beta-sheet, and 40% random coil. The characteristic amphiphilic alpha-helix of cytolysin is located at the N-terminus of the processed Src I.
Jiang
Xiao-Yu
XY
The Open Laboratory for Marine Functional Genomics of National High-Tech Development, Department of Biochemistry, College of Life Sciences, Zhongshan (Sun Yat-Sen) University, Guangzhou 510275, People's Republic of China.
Yang
Wen-li
WL
Chen
Hui-Ping
HP
Tu
Hong-Bin
HB
Wu
Wen-Yan
WY
Wei
Jian-Wen
JW
Wang
Juan
J
Liu
Wen-Hua
WH
Xu
An-Long
AL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003064:Cnidarian Venoms / Q000235:genetics* / Q000633:toxicity*; D017931:DNA Primers; D018076:DNA, Complementary / Q000737:chemistry; D006460:Hemolysin Proteins / Q000235:genetics / Q000633:toxicity; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012615:Sea Anemones
D003064:Cnidarian Venoms; D017931:DNA Primers; D018076:DNA, Complementary; D006460:Hemolysin Proteins