title: Suppression and augmentation of rat adjuvant arthritis with monoclonal anti-interferon-gamma antibody.
abstract: The effects of a monoclonal antibody (MoAb DB-1), which neutralized rat interferon-gamma (IFN-gamma), on the induction and progression of adjuvant arthritis in Lewis rats induced by intraplantar injection of Freund's complete adjuvant was studied. The animals were treated intraperitoneally with MoAb DB-1 (0.3-5 mg) for various times. Prophylactic treatment with MoAb DB-1, starting 2 days prior to arthritis induction, inhibited oedema in both the injected and non-injected hind paws and delayed joint destruction as shown by radiography. However, despite continued MoAb treatment, the disease progressed. High doses of MoAb DB-1 exacerbated the disease. A control MoAb of the same isotype did not have significant effects on adjuvant arthritis. Therapeutic treatment with the MoAb DB-1 starting 8 days after arthritis induction caused only slight and short-lived inhibitory effects. IFN-gamma appears to be a critical lymphokine for the development of adjuvant arthritis.
authors.affiliation: Department of Inflammation/Bone/Allergy, Pharmaceuticals Research Division, Ciba-Geigy, Basle, Switzerland.
authors.forename: I
authors.initials: I
authors.lastname: Wiesenberg
authors.forename: P H
authors.initials: PH
authors.lastname: Van der Meide
authors.forename: H
authors.initials: H
authors.lastname: Schellekens
authors.forename: S
authors.initials: S
authors.lastname: Alkan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D001169:Arthritis, Experimental / Q000209:etiology* / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D005620:Freund's Adjuvant / Q000008:administration & dosage; D007371:Interferon-gamma / Q000037:antagonists & inhibitors* / Q000276:immunology; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D000911:Antibodies, Monoclonal; D007371:Interferon-gamma; D005620:Freund's Adjuvant