title: Interplay of TBP inhibitors in global transcriptional control.
abstract: The TATA binding protein (TBP) is required for the expression of nearly all genes and is highly regulated both positively and negatively. Here, we use DNA microarrays to explore the genome-wide interplay of several TBP-interacting inhibitors in the yeast Saccharomyces cerevisiae. Our findings suggest the following: The NC2 inhibitor turns down, but not off, highly active genes. Autoinhibition of TBP through dimerization contributes to transcriptional repression, even at repressive subtelomeric regions. The TAND domain of TAF1 plays a primary inhibitory role at very few genes, but its function becomes widespread when other TBP interactions are compromised. These findings reveal that transcriptional output is limited in part by a collaboration of different combinations of TBP inhibitory mechanisms.
authors.affiliation: Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16803, USA.
authors.forename: Carmelata
authors.initials: C
authors.lastname: Chitikila
authors.forename: Kathryn L
authors.initials: KL
authors.lastname: Huisinga
authors.forename: Jordan D
authors.initials: JD
authors.lastname: Irvin
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Basehoar
authors.forename: B Franklin
authors.initials: BF
authors.lastname: Pugh
mesh_terms: D001665:Binding Sites; D019281:Dimerization; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics; D016681:Genome, Fungal; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D010750:Phosphoproteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship; D035181:TATA-Box Binding Protein / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics; D016615:Telomere / Q000235:genetics; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: C409889:ARN2 protein, S cerevisiae; D026901:Membrane Transport Proteins; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors; C075995:down-regulator of transcription 1