Detection and relocation of rare events. A comparative study using the laser scanning cytometer and the Metafer/RCDetect microscope scanning system.
We compared instrumental analysis of enriched cord blood nucleated red blood cells (CB-NRBC) out of in vitro contamination preparations of dilutions of minute volumes of male cord blood into peripheral blood from nonpregnant women. This was done using the laser scanning cytometer (LSC) and the Metafer/RCDetect microscope scanning system, both allowing for relocation of positive cells defined on the basis of fluorescence parameters. Both instruments were efficient in performing scanning and relocation; a difference in the recovery of CB-NRBC was not significant and can be explained by the method of preparation used.
Hennerbichler
Simone
S
Institute for Histology and Embryology, Karl-Franzens-University, Harrachgasse 21, A-8010 Graz, Austria.
Méhes
Gabor
G
Schmied
Rudolf
R
Wintersteiger
Reinhold
R
Dohr
Gottfried
G
Ambros
Peter
P
Sedlmayr
Peter
P
D000818:Animals; D002469:Cell Separation / Q000295:instrumentation*; D019544:Equipment Failure Analysis; D004900:Erythroblasts / Q000145:classification* / Q000166:cytology*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D019044:Image Cytometry / Q000295:instrumentation; D017404:In Situ Hybridization, Fluorescence; D007231:Infant, Newborn; D007834:Lasers; D008297:Male; D008431:Maternal-Fetal Exchange; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D011247:Pregnancy; D014998:Y Chromosome