title: Determination of diminazene aceturate in pharmaceutical formulations by HPLC and identification of related substances by LC/MS.
abstract: A validated, reversed-phase, isocratic high-performance liquid chromatographic method for the simultaneous assay of diminazene aceturate, antipyrine (excipient) and diminazene impurities in pharmaceutical formulations is described. The chromatographic system consisted of a Lichrospher-60 RP-select B column with a mobile phase composition of acetonitrile-methanol-ammonium formate (pH 4.0, 20 mM) (10:10: 80 v/v/v) and UV detection at 254 nm. The method is specific, precise and accurate for the determination of diminazene in the presence of its manufacturing and degradation impurities with a limit of detection and quantification of 50 ng/ml and 10 microgram/ml (RSD<3.0%), respectively. The major manufacturing impurity [1-(4 amidino phenyl)3-(4 carbamoyl phenyl)-triazene] and a degradant (p-aminobenzamidine) of diminazene aceturate have been resolved and identified by liquid chromatography/electrospray ionization-mass spectrometry operated in a positive ion mode.
authors.affiliation: Department of Pharmaceutical Sciences, University of Strathclyde, 27 Taylor, Street, G4 0NR, Glasgow, UK.
authors.forename: C
authors.initials: C
authors.lastname: Atsriku
authors.forename: D G
authors.initials: DG
authors.lastname: Watson
authors.forename: J N A
authors.initials: JN
authors.lastname: Tettey
authors.forename: M H
authors.initials: MH
authors.lastname: Grant
authors.forename: G G
authors.initials: GG
authors.lastname: Skellern
mesh_terms: D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D004133:Diminazene / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: C003915:diminazene aceturate; D004133:Diminazene