title: Local inflammatory reaction induced by Bothrops jararaca venom differs in mice selected for acute inflammatory response.
abstract: Bothrops jararaca venom (BjV) causes severe systemic and local reactions, characterized by an acute inflammatory reaction with accumulation of leukocytes and release of endogenous mediators. The systemic and local effects of BjV were compared in lines of mice genetically selected for maximal (AIR(max)) or minimal (AIR(min)) acute inflammatory reactivity (AIR). The systemic reaction was evaluated by LD(50) and the local reaction by edema formation, cellular influx, release of PGE(2), NO and H(2)O(2) and the production of the pro-inflammatory cytokines IL-6, Tumor necrosis factor-alpha (TNF-alpha) and IFN-gamma. Both mouse lines were equally susceptible to the lethal effects of the venom showing similar LD(50) but differed significantly in terms of the local inflammatory reaction. Footpad edema and leukocyte influx in the peritoneum after BjV inoculation was higher in AIR(max) compared to AIR(min), BALB/c or outbred Swiss mice. Coincidently, higher levels of the soluble mediators PGE(2), IFN-gamma and TNF-alpha were detected in the inflammatory exudate induced by BjV in AIR(max) mice. Cytokines levels were correlated to in vitro NO and H(2)O(2) production. The results demonstrate that the genetic factors selected in AIR(max) and AIR(min) lines of mice interfere in the control of the acute local reaction triggered by BjV venom.
authors.affiliation: Laboratório de Imunogenética, Instituto Butantan, Av Vital Brasil 1500-cep 05503-900 São Paulo, SP, Brazil.
authors.forename: Adriana S
authors.initials: AS
authors.lastname: Carneiro
authors.forename: Orlando G
authors.initials: OG
authors.lastname: Ribeiro
authors.forename: Marcelo
authors.initials: M
authors.lastname: De Franco
authors.forename: Wafa H K
authors.initials: WH
authors.lastname: Cabrera
authors.forename: Francisca
authors.initials: F
authors.lastname: Vorraro
authors.forename: Maria
authors.initials: M
authors.lastname: Siqueira
authors.forename: Olga M
authors.initials: OM
authors.lastname: Ibañez
authors.forename: Nancy
authors.initials: N
authors.lastname: Starobinas
mesh_terms: D000818:Animals; D017837:Bothrops; D003435:Crotalid Venoms / Q000008:administration & dosage / Q000633:toxicity*; D016207:Cytokines / Q000187:drug effects; D015232:Dinoprostone / Q000378:metabolism; D004487:Edema / Q000139:chemically induced / Q000473:pathology; D005260:Female; D006861:Hydrogen Peroxide / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced* / Q000473:pathology; D007274:Injections, Intraperitoneal; D007371:Interferon-gamma / Q000187:drug effects; D015850:Interleukin-6 / Q000378:metabolism; D007928:Lethal Dose 50; D007962:Leukocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D009569:Nitric Oxide / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects
chemical_list: D003435:Crotalid Venoms; D016207:Cytokines; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D007371:Interferon-gamma; D006861:Hydrogen Peroxide; D015232:Dinoprostone