title: BACFinder: genomic localisation of large insert genomic clones based on restriction fingerprinting.
abstract: We have developed software that allows the prediction of the genomic location of a bacterial artificial chromosome (BAC) clone, or other large genomic clone, based on a simple restriction digest of the BAC. The mapping is performed by comparing the experimentally derived restriction digest of the BAC DNA with a virtual restriction digest of the whole genome sequence. Our trials indicate that this program identified the genomic regions represented by BAC clones with a degree of accuracy comparable to that of end-sequencing, but at considerably less cost. Although the program has been developed principally for use with Arabidopsis BACs, it should align large insert genomic clones to any fully sequenced genome.
authors.affiliation: John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK.
authors.forename: Mark L
authors.initials: ML
authors.lastname: Crowe
authors.forename: Debashis
authors.initials: D
authors.lastname: Rana
authors.forename: Fiona
authors.initials: F
authors.lastname: Fraser
authors.forename: Ian
authors.initials: I
authors.lastname: Bancroft
authors.forename: Martin
authors.initials: M
authors.lastname: Trick
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D020137:Base Pair Mismatch / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular; D020451:Contig Mapping / Q000379:methods; D016172:DNA Fingerprinting / Q000379:methods*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D016678:Genome; D018745:Genome, Plant; D015698:Genomic Library; D020161:Physical Chromosome Mapping / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity; D012984:Software
chemical_list: D004262:DNA Restriction Enzymes