Transcription initiation sites and promoter structure of the human TRAIL-R3 gene.
TRAIL-R3 is a decoy receptor for TRAIL (tumor necrosis factor-related apoptosis-inducing ligand), a member of the tumor necrosis factor ligand family. In several cell types decoy receptors inhibit TRAIL-induced apoptosis by binding TRAIL and preventing its binding to TRAIL pro-apoptotic receptors. Here we report the cloning of the promoter region of human TRAIL-R3 and the mapping of the transcriptional start sites. This gene contains a consensus TATA box and the minimal promoter lies within the first 33 nucleotides upstream of the transcription start site. Transient transfection assays of luciferase reporter plasmids demonstrate that human TRAIL-R3 promoter can be induced in doxorubicin-treated MCF-7 cells in a p53-independent manner.
Ruiz de Almodóvar
Carmen
C
Instituto de Parasitología y Biomedicina, CSIC, calle Ventanilla 11, Granada, Spain.
López-Rivas
Abelardo
A
Redondo
Juan Miguel
JM
Rodríguez
Antonio
A
D024506:5' Flanking Region; D000970:Antineoplastic Agents / Q000494:pharmacology; D001483:Base Sequence; D003001:Cloning, Molecular; D004317:Doxorubicin / Q000494:pharmacology; D058851:GPI-Linked Proteins; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis / Q000235:genetics*; D053320:Receptors, Tumor Necrosis Factor, Member 10c; D024363:Transcription Initiation Site; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured; D053319:Tumor Necrosis Factor Decoy Receptors; D016159:Tumor Suppressor Protein p53 / Q000502:physiology
D000970:Antineoplastic Agents; D058851:GPI-Linked Proteins; D012333:RNA, Messenger; D018124:Receptors, Tumor Necrosis Factor; D053320:Receptors, Tumor Necrosis Factor, Member 10c; C108661:TNFRSF10C protein, human; D053319:Tumor Necrosis Factor Decoy Receptors; D016159:Tumor Suppressor Protein p53; D004317:Doxorubicin