Characterization of a novel plasmid pXZ608 from Corynebacterium glutamicum.
The complete nucleotide sequence of a novel cryptic plasmid pXZ608 from Corynebacterium glutamicum 227 was determined. pXZ608 was 5949 bp with six open reading frames (ORF1-6). The predicted ORF1 gene product was homologous to replication proteins of rolling circle replication plasmids. The conserved single- and double-stranded origins of rolling circle replication were found, and interestingly, the two origins were both located on ORF1, which indicated that the Rep protein encoded by ORF1 could bind to its own gene region. Deletion analysis revealed that the minimal replicon was located on the 2.14-kb SacI-BstEII fragment.
Lei
Chengxiang
C
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, PR China.
Ren
Zhaorui
Z
Yang
Wen
W
Chen
Yuan
Y
Chen
Dan
D
Liu
Mingqiu
M
Yan
Weiyao
W
Zheng
Zhaoxin
Z
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003352:Corynebacterium / Q000235:genetics*; D004269:DNA, Bacterial / Q000096:biosynthesis; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D012093:Replicon; D016415:Sequence Alignment
D001426:Bacterial Proteins; D004269:DNA, Bacterial