Use of a single monoclonal antibody to determine the susceptibilities of herpes simplex virus type 1 and type 2 clinical isolates to acyclovir.
This report describes a flow cytometry drug susceptibility assay that uses a single fluorochrome-labeled monoclonal antibody to determine the acyclovir susceptibilities of herpes simplex virus (HSV) type 1 or type 2 clinical isolates. This assay yields 50% effective doses (drug concentrations that reduce the number of antigen-positive cells by 50%) for HSV clinical isolates that are equivalent to those obtained with the plaque reduction assay.
Chutkowski
Christine
C
Center for Immunology and Microbial Disease, Albany Medical Center, Albany, New York 12208, USA.
Olson
Betty
B
McDonough
Ann
A
Mahoney
James
J
McSharry
James J
JJ
D000212:Acyclovir / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis*; D000998:Antiviral Agents / Q000494:pharmacology*; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000276:immunology; D018258:Herpesvirus 2, Human / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000998:Antiviral Agents; D005456:Fluorescent Dyes; D000212:Acyclovir