Possible coupling of prostaglandin E receptor EP(1) to TRP5 expressed in Xenopus laevis oocytes.
We previously reported that the prostaglandin E(2) (PGE(2)) receptor subtype EP(1) is coupled to intracellular Ca(2+) mobilization in CHO cells, which is dependent on extracellular Ca(2+) in a pertussis toxin-insensitive manner [H. Katoh, et al., Biochim. Biophys. Acta 1244 (1995) 41-48]. However, it remains unknown about the signal transduction involved in this response. To investigate the mechanism regulating Ca(2+) mobilization mediated by EP(1) receptors in detail, we performed a series of experiments using the Xenopus laevis oocyte expression system and found that endogenous G(q) and/or G(11), and not G(i1) is involved in the Ca(2+) mobilization induced by PGE(2). We further investigated the receptor-activated Ca(2+) channel (RACC)-related response by introducing mRNA for mouse transient receptor potential 5 (TRP5), a possible candidate for the RACC, and found effective coupling between them. These results suggest that the EP(1) receptors induce Ca(2+) mobilization via G(q) and/or G(11) and Ca(2+) influx via TRP.
Tabata
Hiroyuki
H
Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.
Tanaka
Satoshi
S
Sugimoto
Yukihiko
Y
Kanki
Hideaki
H
Kaneko
Shuji
S
Ichikawa
Atsushi
A
D000818:Animals; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism*; D027682:Cation Transport Proteins; D006224:Cricetinae; D017931:DNA Primers; D015232:Dinoprostone / Q000378:metabolism; D015640:Ion Channel Gating; D051379:Mice; D016376:Oligonucleotides, Antisense; D009865:Oocytes / Q000378:metabolism; D011485:Protein Binding; D018078:Receptors, Prostaglandin E / Q000378:metabolism*; D015398:Signal Transduction; D050052:TRPC Cation Channels; D014982:Xenopus laevis
D015220:Calcium Channels; D027682:Cation Transport Proteins; D017931:DNA Primers; D016376:Oligonucleotides, Antisense; D018078:Receptors, Prostaglandin E; D050052:TRPC Cation Channels; C112021:Trpc5 protein, mouse; D015232:Dinoprostone; D002118:Calcium