title: The clinical relevance of an isolated increase in the number of circulating hyperchromic red blood cells.
abstract: OBJECTIVE
  To search for laboratory evidence of hereditary spherocytosis (HS) among apparently healthy children with the chance finding of an isolated increase in hyperchromic red cells (cells with intracellular haemoglobin concentration > 410 g/litre).
  
  
  METHODS
  Blood and reticulocyte counts and Pink tests were performed on successive children found on routine counts to have > 4% hyperchromic red cells, and compared with age and mean cell haemoglobin concentration (MCHC) matched controls and children known to have HS.
  
  
  RESULTS
  Thirty four patients with > 4% hyperchromic red cells had significantly higher absolute numbers of such cells (p < 0.0001) and higher reticulocyte counts (p < 0.01) than age matched controls, together with higher MCHC (p < 0.0001) and haemoglobin distribution width (p < 0.0001) values and lower mean cell volume (p < 0.02) values. Significant differences were also found among hyperchromic red blood cell, reticulocyte, and haemoglobin distribution width values when subjects were compared with MCHC matched controls. Pink test values were higher in children with increased hyperchromic red blood cells, but not significantly so. In patients with HS, most variables measured were significantly different both from those of children with > 4% hyperchromic cells and controls. Despite the differences found, few MCHC, HDW, reticulocyte, or Pink test values were outside of the normal limits, and only one child with increased hyperchromic cells had both a mild reticulocytosis and a slightly raised Pink test value.
  
  
  CONCLUSIONS
  Subjects with an isolated increase in hyperchromic red blood cells have a profile of red blood cell changes similar to that of patients with HS, but to a lesser degree. They may carry a recessive form of the disease but lack the laboratory features of clinically manifest HS.
authors.affiliation: Department of Paediatric Haematology, Sheffield Children's Hospital NHS Trust, Sheffield S10 2TH, UK.
authors.forename: A M
authors.initials: AM
authors.lastname: Conway
authors.forename: A J
authors.initials: AJ
authors.lastname: Vora
authors.forename: R F
authors.initials: RF
authors.lastname: Hinchliffe
mesh_terms: D000293:Adolescent; D001772:Blood Cell Count; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004906:Erythrocyte Count; D004911:Erythrocyte Volume; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007223:Infant; D012016:Reference Values; D017701:Reticulocyte Count; D013103:Spherocytosis, Hereditary / Q000097:blood / Q000175:diagnosis*
chemical_list: D006454:Hemoglobins