Structural investigation of the O-specific polysaccharides of Morganella morganii consisting of two higher sugars.
The lipopolysaccharide of the bacterium Morganella morganii (strain KF 1676, RK 4222) yielded two polysaccharides, PS1 and PS2, when subjected to mild acid degradation followed by GPC. The polysaccharides were studied by 1H and 13C NMR spectroscopy, including two-dimensional COSY, TOCSY, NOESY, 1H,(13)C HMQC, and HMBC experiments. Each polysaccharide was found to contain a disaccharide repeating unit consisting of two higher sugars, 5-acetamidino-7-acetamido-3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic acid (a derivative of 8-epilegionaminic acid, 8eLeg5Am7Ac) and 2-acetamido-4-C-(3'-carboxamide-2',2'-dihydroxypropyl)-2,6-dideoxy-D-galactose (shewanellose, She). The two polysaccharides differ only in the ring size of shewanellose and have the following structures:Shewanellose has been previously identified in a phenol-soluble polysaccharide from Shewanella putrefaciens A6, which shows a close structural similarity to PS2.
Kilcoyne
Michelle
M
Department of Chemistry, National University of Ireland, Galway, Ireland. angela.savage@nuigalway.ie
Shashkov
Alexander S
AS
Senchenkova
Sof'ya A
SA
Knirel
Yuriy A
YA
Vinogradov
Evgeny V
EV
Radziejewska-Lebrecht
Joanna
J
Galimska-Stypa
Regina
R
Savage
Angela V
AV
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D014644:Genetic Variation; D008969:Molecular Sequence Data; D020613:Morganella morganii / Q000737:chemistry* / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry* / Q000378:metabolism; D012794:Sialic Acids / Q000032:analysis / Q000737:chemistry
D019081:O Antigens; D012794:Sialic Acids