[Antisense inhibition of cyclooxygenase-2 reduces malignant phenotype of gastric cancer cells].
OBJECTIVE
To investigate the role of cyclooxygenase-2 (COX-2) in tumorigenesis of gastric cancer, and the introduction of into human gastric cancer cells to suppress COX-2 expression.


METHODS
The high COX-2 expressing human gastric cancer cell line SGC 7901 was stably transfected with the COX-2 antisense recombinant vector and plain vector (named as 7901-AS and 7901-P cells). The COX-2 expression levels in transfected cells were detected by immunocytochemistry and dot blotting methods. Proliferation and tumorigenic ability of transfected (7901-AS, 7901-P) and control (SGC 7901) cells were evaluated in vitro with MTT assay and in vivo with nude mice.


RESULTS
Antisense treatment for COX-2 gene significantly reduced the expression level of COX-2 protein and mRNA and led inhibition of proliferation in 7901-AS cells. The tumor graft of 7901-AS in nude mice 30 days after implantation had less volume and weight than that of SGC7901 and 7901-P [(486.67 +/- 15.28) mg vs (826.67 +/- 77.67) mg and (776.67 +/- 300.06) mg, P < 0.01].


CONCLUSIONS
Overexpression of COX-2 in human gastric cancer cell line SGC 7901 was related to the malignant phenotype of cancer cells. Inhibition of COX-2 expression by antisense technique could reverse malignant phenotypes of gastric cancer cells.
Wu
Hanping
H
Institute of Digestive Disease, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Wu
Kaichun
K
Yao
Liping
L
Xu
Meihong
M
Fan
Daiming
D
D000818:Animals; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D051546:Cyclooxygenase 2; D016373:DNA, Antisense / Q000235:genetics* / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D010641:Phenotype; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured
D016373:DNA, Antisense; D007527:Isoenzymes; D008565:Membrane Proteins; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases