Gamma-tubulin37C and gamma-tubulin ring complex protein 75 are essential for bicoid RNA localization during drosophila oogenesis.
bicoid (bcd) RNA localization requires the activity of exuperantia and swallow at sequential steps of oogenesis and is microtubule dependent. In a genetic screen, we identified two novel genes essential for bcd RNA localization. They encode maternal gamma-Tubulin37C (gammaTub37C) and gamma-tubulin ring complex protein 75 (Dgrip75), both of which are gamma-tubulin ring complex components. Mutations in these genes specifically affect bcd RNA localization, whereas other microtubule-dependent processes during oogenesis are not impaired. This provides direct evidence that a subset of microtubules organized by the gamma-tubulin ring complex is essential for localization of bcd RNA. At stage 10b, we find gammaTub37C and Dgrip75 anteriorly concentrated and propose the formation of a microtubule-organizing center at the anterior pole of the oocyte.
Schnorrer
Frank
F
Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstr. 35, 72076, Tübingen, Germany. frank.schnorrer@tuebingen.mpg.de
Luschnig
Stefan
S
Koch
Iris
I
Nüsslein-Volhard
Christiane
C
D000818:Animals; D018385:Centrosome / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D005260:Female; D017344:Genes, Insect; D049452:Green Fluorescent Proteins; D018398:Homeodomain Proteins / Q000235:genetics*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D022101:Microtubule-Organizing Center / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D016296:Mutagenesis; D009865:Oocytes / Q000378:metabolism; D009866:Oogenesis / Q000502:physiology*; D012313:RNA / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics*; D014404:Tubulin / Q000235:genetics / Q000378:metabolism*
D029721:Drosophila Proteins; C469730:Grip75 protein, Drosophila; D018398:Homeodomain Proteins; D008164:Luminescent Proteins; D008869:Microtubule-Associated Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014404:Tubulin; C056478:bcd protein, Drosophila; C469729:gammaTub37C protein, Drosophila; C073970:swa protein, Drosophila; D049452:Green Fluorescent Proteins; D012313:RNA