title: Preliminary studies on insecticidal activities of actinomycete strains propagated on solid and broth media using Musca domestica (Diptera: Muscidae).
abstract: Forty-one actinomycete soil isolates were propagated on solid media and subjected to a screening program for their insecticidal activities. These strains were studied to evaluate their efficiency as biocontrol agents against third larval instar of the house fly Musca domestica. Two groups of larvae were fed on either artificial media with the organisms studied (group I), or on artificial media inoculated with the secondary metabolites (group II). Among the strains selected for biological activity screening 15 strains of group I affected significantly the larval duration of the examined insects, compared to 18 strains of group II. This increase ranged from 7.2 +/- 0.5 to 11.1 +/- 4.2 for group I and from 7.1 +/- 0.3 to 17.5 +/- 4.81 for group II compared to 7.0 +/- 0.5 of controls. The lethal effects of some strains were recorded when the strain no 27 caused the highest mortality percentage (80%) in group I, while 55% mortality was recorded for group II using the secondary metabolites (broth media) of strain 20. In addition, many strains affected the pupal and adult stages expressing latent effects of their strains. A slight reduction in larval duration was recorded for strain 22. Streptomyces and Streptoverticillium were recorded as the most effective genera.
authors.affiliation: Botany and Microbiology Department, Al-Azhar University, Madinet Nasr, Cairo, Egypt.
authors.forename: S A
authors.initials: SA
authors.lastname: Ghazal
authors.forename: A S
authors.initials: AS
authors.lastname: Bream
authors.forename: Z K
authors.initials: ZK
authors.lastname: Abd el-Aziz
authors.forename: S Y
authors.initials: SY
authors.lastname: Ibrahim
mesh_terms: D039903:Actinobacteria / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D000898:Antibiosis; D003470:Culture Media; D006793:Houseflies / Q000254:growth & development / Q000382:microbiology*; D007306:Insecticides / Q000378:metabolism / Q000494:pharmacology*; D007814:Larva; D008018:Life Cycle Stages; D008826:Microbial Sensitivity Tests; D010572:Pest Control, Biological; D012988:Soil Microbiology
chemical_list: D003470:Culture Media; D007306:Insecticides