Maturational and developmental competence of cumulus-free immature human oocytes derived from stimulated and intracytoplasmic sperm injection cycles.
The present experiments compared the maturational and developmental competence of immature oocytes derived from stimulated cycles, following culture in a newly designed in-vitro maturation medium (IVM-medium) or in standard tissue culture medium (TCM-199; control). The results indicated that maturation and fertilization rates were comparable when the cumulus-free M-I stage oocytes were matured in the IVM-medium (78.6%) or the control medium (70.8%). However, there was a significant difference in blastocyst development (P < 0.05) when M-I oocytes were matured in these two media (19.6 versus 7.7%). Both maturation and early embryonic development rates of GV-stage oocytes were significantly higher (P < 0.01) in the IVM-medium (maturation: 75.7%; blastocyst: 12.9%) compared with control (maturation 55.7%; blastocyst: 0.0%). Moreover, embryos developed to the blastocyst stage at a higher rate in both media if GV-stage oocytes had matured within 24 h compared with 48 h of culture. These results demonstrate that immature human oocytes derived from stimulated ovaries can achieve maturation and early embryonic development in vitro, especially in the new IVM-medium, which may allow additional embryos to be produced for clinical use at embryo transfer.
Chian
R C
RC
Department of Obstetrics and Gynecology, McGill University, Montreal, Montreal, Quebec, Canada. ri-cheng.chian@muhc.mcgill.ca
Tan
S L
SL
D001755:Blastocyst / Q000166:cytology*; D002453:Cell Cycle; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D006801:Humans; D008297:Male; D008677:Metaphase; D009865:Oocytes / Q000166:cytology* / Q000502:physiology; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*
D003470:Culture Media