The partially degraded lipopolysaccharide of Burkholderia cepacia and ornithine-containing lipids derived from some Gram-negative bacteria are useful complex lipid adjuvants.
The partially degraded lipopolysaccharide of Burkholderia cepacia (LPSdegr) and the ornithine-containing lipids were purified from some bacteria. The substances were developed as complex lipid adjuvants, because they have weak toxicity and are able to activate the immune systems of the living body. After various toxoid antigens such as pertussis toxoid, diphtheria toxoid and tetanus toxoid were mixed with the complex lipid adjuvants, the mixtures were administered to mice subcutaneously. Antitoxoid IgG antibody titers in the serum were measured several times over 3 months. The efficacy of the LPSdegr as adjuvant was almost as high as that of the ornithine-containing lipids, and it was almost equal to that of the aluminum hydroxide adjuvant (Alum), which is generally used as a vaccine adjuvant.
Kawai
Yohko
Y
Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1-23-1, Toyama-cho, Shinjuku-ku, Tokyo 162-8640, Japan.
Watanabe
Mineo
M
Matsuura
Motohiro
M
Nishijima
Masahiro
M
Kawahara
Kazuyoshi
K
D000276:Adjuvants, Immunologic / Q000494:pharmacology* / Q000627:therapeutic use; D000534:Alum Compounds / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D016956:Burkholderia cepacia / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D004168:Diphtheria Toxoid / Q000276:immunology; D006090:Gram-Negative Bacteria / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009952:Ornithine / Q000032:analysis*; D013208:Staphylococcal Toxoid / Q000276:immunology; D013745:Tetanus Toxoid / Q000276:immunology
D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D004168:Diphtheria Toxoid; D015850:Interleukin-6; D008055:Lipids; D008070:Lipopolysaccharides; D013208:Staphylococcal Toxoid; D013745:Tetanus Toxoid; C041524:aluminum sulfate; D009952:Ornithine