On-line liquid-chromatography-nuclear magnetic resonance spectroscopy-mass spectrometry coupling for the separation and characterization of secoisolariciresinol diglucoside isomers in flaxseed.
Two secoisolariciresinol diglucoside (SDG) diastereomers were extracted from flaxseed and liberated through alkaline hydrolysis. Anion-exchange and reversed-phase chromatography were successfully employed to purify the hydrolyzed flaxseed extract. On-line LC-NMR-MS analyses revealed the structure of the isolated and purified SDG diastereomers, [2R,2'R]-2,3-bis[(4-hydroxy-3-methoxyphenyl)-methyl]-1,4-butanediyl-bis-beta-glucopyranoside the predominant flaxseed lignan and [2R,2'S]-2,3-bis[(4-hydroxy-3-methoxyphenyl)methyl]-1,4-butanediyl-bis-beta-glucopyranoside, a previously incompletely characterized minor flaxseed lignan. Circular dichroism (CD) analyses confirmed the presence of two distinguished optically active compounds present in the flaxseed extract.
Fritsche
J
J
Unilever Research & Development, Vlaardingen, The Netherlands.
Angoelal
R
R
Dachtler
M
M
D002072:Butylene Glycols / Q000032:analysis* / Q000302:isolation & purification; D002942:Circular Dichroism; D019597:Flax / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis* / Q000302:isolation & purification; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*
D002072:Butylene Glycols; D005960:Glucosides; C090142:secoisolariciresinol diglucoside