Cytoskeletal proteins and resident flora.
Recent observations demonstrate that enteropathogenetic and enterohaemorrhagic bacteria, as well as other non enteropathogenetic bacteria (Listeria, Coxiella Burnetii), may subvert the host cell cytoskeleton. Models from enteropathogenic bacteria demonstrate that cytoskeletal proteins are required for bacteria binding to the enterocytes and that they play a role in the immune response of the host to intestinal bacteria. The cytoskeletal protein family Tropomyosins is present in all eukaryotic cells, with multiple isoforms regulated by multiple genes. Of the different Tropomyosin isoforms, TM5 has been shown to be expressed in colonic and jejunal epithelial cells, while TM1 in colonic and jejunal smooth muscle. In vitro studies have shown the presence of serum and mucosal IgG against TM5 in almost two thirds of patients with ulcerative colitis, suggesting: a. a possible autoimmune response to Tropomyosin in these patients; b. the hypothesis that the development of pouchitis may be related to the expression of TM5 in the ileal pouch; c. the use of probiotics in the treatment of pouchitis. Overall, the new expression of cytoskeletal proteins on the cell surface appears to be possibly induced by several mechanisms, including intestinal bacteria and apoptosis. The expression of cytoskeletal proteins on the cell surface may induce tolerance or autoimmune response on target cells. Further investigations are, however needed on the possible role of cytoskeletal proteins in human diseases.
Biancone
L
L
Chair of Gastroenterology, Tor Vergata University of Rome, Italy. biancone@med.uniroma2.it
Palmieri
G
G
Lombardi
A
A
Vavassori
P
P
Monteleone
I
I
Del Vecchio Blanco
G
G
Colantoni
A
A
Spagnoli
L
L
Tonelli
F
F
Pallone
F
F
D003598:Cytoskeletal Proteins / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D019449:Pouchitis / Q000209:etiology / Q000628:therapy; D019936:Probiotics / Q000627:therapeutic use; D014335:Tropomyosin / Q000378:metabolism
D003598:Cytoskeletal Proteins; C498783:TPM3 protein, human; D014335:Tropomyosin