Transforming growth factor-beta (TGF-beta) and tissue transglutaminase expression in the small intestine in children with coeliac disease.
The production of cytokines from T cells and macrophages is of potential importance for the histological changes apparent in coeliac disease (CoD). Small intestinal biopsy specimens from children with CoD and disease control subjects were investigated for their content of cytokines and tissue transglutaminase (tTG). The transforming growth factor-beta1 (TGF-beta1) expression was increased in the lamina propria of children with villous atrophy. In contrast, TGF-beta3 was expressed at a higher level in the epithelium and the lamina propria of the disease control subjects. The tTG expression was increased in the small intestine of CoD patients as compared with that in subjects. Interleukin-4 (IL-4) was detected in the lamina propria of both CoD patients and controls, and some of the investigated biopsy specimens also showed IL-4 expression in the epithelium. We conclude that children with active CoD could have an altered expression of TGF-beta and tTG in the small intestine and that a disturbed regulation of TGF-beta may be of importance in the immune pathogenesis of CoD.
Hansson
T
T
Department of Rheumatology, Karolinska University Hospital, Stockholm; Pharmacia Diagnostics; and Department of Pediatrics, Uppsala University Hospital, Uppsala, Sweden. tony.hansson@cmm.ki.se
Ulfgren
A-K
AK
Lindroos
E
E
DannAEus
A
A
Dahlbom
I
I
Klareskog
L
L
D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology; D016022:Case-Control Studies; D002446:Celiac Disease / Q000201:enzymology* / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D004848:Epithelium / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D005260:Female; D019204:GTP-Binding Proteins / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D015847:Interleukin-4 / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D007421:Intestine, Small / Q000201:enzymology* / Q000276:immunology* / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000473:pathology; D008297:Male; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*; D053773:Transforming Growth Factor beta1; D053782:Transforming Growth Factor beta3; D011503:Transglutaminases / Q000096:biosynthesis*; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D019204:GTP-Binding Proteins; D015847:Interleukin-4; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D053782:Transforming Growth Factor beta3; D011503:Transglutaminases; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C508960:TGFB1 protein, human