title: Development of Vero cell-derived inactivated Japanese encephalitis vaccine.
abstract: We have established a manufacturing system for a Vero cell-derived inactivated Japanese encephalitis vaccine at a 500l scale. The production system involves expansion of Vero cells using microcarrier, followed by virus infection. Except for an additional purification step, the downstream purification processes are similar to those used for the current mouse brain-derived vaccine; cell removal, concentration and removal of low-molecular weight impurities by membrane filtration, formalin-inactivation, sucrose density gradient ultracentrifugation, and Sulfate-Cellulofine column chromatography are conducted. The antigen obtained from the manufacturing system was highly purified and its physico-chemical and immunological properties were comparable with those of antigen derived from mouse brains. Our system is very simple and could be easily scaled-up to allow vaccine production at a several thousand litre scale.
authors.affiliation: Kikuchi Research Center, The Chemo-Sero Therapeutic Research Institute, Kumamoto 869-1298, Japan. sugawara@kaketsuken.or.jp
authors.forename: Keishin
authors.initials: K
authors.lastname: Sugawara
authors.forename: Kiyoto
authors.initials: K
authors.lastname: Nishiyama
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: Motoharu
authors.initials: M
authors.lastname: Abe
authors.forename: Kengo
authors.initials: K
authors.lastname: Sonoda
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Komatsu
authors.forename: Yoshikane
authors.initials: Y
authors.lastname: Horikawa
authors.forename: Kengo
authors.initials: K
authors.lastname: Takeda
authors.forename: Tomitaka
authors.initials: T
authors.lastname: Honda
authors.forename: Shoji
authors.initials: S
authors.lastname: Kuzuhara
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Kino
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizokami
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Mizuno
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Oka
authors.forename: Kennosuke
authors.initials: K
authors.lastname: Honda
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002522:Chlorocebus aethiops; D004664:Encephalitis Virus, Japanese / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure; D004797:Enzyme-Linked Immunosorbent Assay; D005285:Fermentation; D022321:Japanese Encephalitis Vaccines / Q000276:immunology / Q000302:isolation & purification*; D051379:Mice; D008854:Microscopy, Electron; D009500:Neutralization Tests; D012449:Safety; D015164:Vaccines, Inactivated / Q000276:immunology / Q000302:isolation & purification; D014709:Vero Cells; D014776:Virus Cultivation
chemical_list: D000956:Antigens, Viral; D022321:Japanese Encephalitis Vaccines; D015164:Vaccines, Inactivated