title: Toward a more complete recognition of immunoreactive antigens in squamous cell lung carcinoma.
abstract: There is very limited knowledge about the antibody response against tumor-expressed antigens in lung cancer. To arrive at a more complete picture of lung cancer antigens, we generated 2 cDNA libraries from squamous cell lung carcinoma and isolated 15 immunogenic antigens using autologous sera. Among the antigens most frequently identified were the lymphoid blast crisis oncogene (LBC), an unknown hypothetical protein and the p53-binding protein (TP53 BP), which have already been associated with tumor development. Of the immunogenic antigens, 6 map to chromosomes that are frequently altered in squamous cell lung carcinoma. SEREX database analysis showed that 7 of the identified immunogenic antigens have been associated with the humoral immune response in other human tumors. Screening with heterologous sera of patients with lung carcinoma identified 4 antigens, including human protein kinase C and TP53 BP, which have also been found by autologous screening. Only 1 of the 15 identified antigens reacted with any of the 36 control sera, which were taken from individuals without known disease. Sera from adenocarcinoma and large cell carcinoma of the lung were not reactive for the antigens. In summary, using 2 newly established cDNA libraries, we isolated 15 novel antigens, which were subsequently evaluated for the frequency of their corresponding antibodies in autologous, normal and heterologous sera; their chromosomal localization; and their correlation with survival after surgery.
authors.affiliation: Department of Human Genetics, Medical School, University of Saarland, Homburg/Saar, Germany.
authors.forename: Isabel
authors.initials: I
authors.lastname: Diesinger
authors.forename: Christine
authors.initials: C
authors.lastname: Bauer
authors.forename: Nicole
authors.initials: N
authors.lastname: Brass
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Schaefers
authors.forename: Nicole
authors.initials: N
authors.lastname: Comtesse
authors.forename: Gerhard
authors.initials: G
authors.lastname: Sybrecht
authors.forename: Eckart
authors.initials: E
authors.lastname: Meese
mesh_terms: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000276:immunology*; D002874:Chromosome Mapping; D004273:DNA, Neoplasm / Q000032:analysis; D015723:Gene Library; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000276:immunology*
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm