title: [The expressions of TGF-beta1 and Smad 2 mRNA on diseased glomeruli and their significance in the development of glomerulosclerosis].
abstract: OBJECTIVE
  To explore the expressions of transforming growth factor-beta1 (TGF-beta1) and its signaling transduction molecule Smad 2 and their significance in the development of glomerulosclerosis.
  
  
  METHODS
  Using in situ hybridization and immunohistochemistry to detect Smad 2 mRNA expression and TGF-beta1, collagen IV, fibronectin expression in renal biopsies from 61 cases with a spectrum of glomerulonephritis including IgA nephropathy (40 cases), membranous glomerulonephritis (10 cases) and sclerosing glomerulonephritis (11 cases), compared with 11 cases of glomerular mild lesion with image analysis system.
  
  
  RESULTS
  With the exception of Smad 2 mRNA expression in mild type IgA nephropathy, all other types of diseased glomeruli showed increased expression of both TGF-beta1 and Smad 2 mRNA when compared with the 11 cases of mild glomerular lesions. The expressions of glomerular TGF-beta1 and Smad 2 mRNA positively correlated with collagen IV and fibronectin deposition in the glomeruli.
  
  
  CONCLUSIONS
  TGF-beta1 and Smad 2 may be involved in the excessive deposition of glomerular extracellular matrix and play an important role in the development of glomerulosclerosis.
authors.affiliation: Department of Pathology, Medical Center of Fudan University, Shanghai 200032, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Ruan
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiurong
authors.initials: X
authors.lastname: Zhang
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.forename: Muyi
authors.initials: M
authors.lastname: Guo
mesh_terms: D024141:Collagen Type IV / Q000032:analysis; D004268:DNA-Binding Proteins / Q000235:genetics*; D005353:Fibronectins / Q000032:analysis; D005921:Glomerulonephritis / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007678:Kidney Glomerulus / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis*; D051899:Smad2 Protein; D015534:Trans-Activators / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000032:analysis*; D053773:Transforming Growth Factor beta1
chemical_list: D024141:Collagen Type IV; D004268:DNA-Binding Proteins; D005353:Fibronectins; D012333:RNA, Messenger; C497782:SMAD2 protein, human; D051899:Smad2 Protein; C508960:TGFB1 protein, human; D015534:Trans-Activators; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1