Hepatitis A virus inhibits cellular antiviral defense mechanisms induced by double-stranded RNA.
The consequences of a hepatitis A virus (HAV) infection on cell-based antiviral responses and the interactions between virus and host cells resulting in persistent infections are poorly understood. In this report, we show that HAV does inhibit double-stranded (dsRNA)-induced beta interferon (IFN-beta) gene expression by influencing the IFN-beta enhanceosome, as well as dsRNA-induced apoptosis, which suggests that both effects may be connected by shared viral and/or cellular factors. This ability of HAV, which preserves the sites of virus production for a longer time, may allow the virus to establish an infection and may be the presupposition for setting up persistent infections. Our results suggest that the inhibitory effect of HAV on the cellular defense mechanisms might not be sufficient to completely prevent the antiviral reactions, which may be induced by accumulating viral dsRNA, at a later stage of infection. However, HAV seems to counteract this situation by downregulation of viral replication and in the following production of viral dsRNA. This ability of noncytopathogenic HAV acts dominantly on cytopathogenic HAV in trans. The downregulation might ensure the moderate replication which seems necessary for inhibition of the antiviral mechanisms by HAV and therefore for the persistent state of the HAV infection.
Brack
Kerstin
K
Department of Virology, University of Bremen, D-28359 Bremen, Germany.
Berk
Iris
I
Magulski
Thomas
T
Lederer
Jörg
J
Dotzauer
Andreas
A
Vallbracht
Angelika
A
D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004742:Enhancer Elements, Genetic; D015870:Gene Expression; D006506:Hepatitis A / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000821:virology; D030041:Hepatitis A virus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D016899:Interferon-beta / Q000096:biosynthesis / Q000235:genetics; D008253:Macaca mulatta; D012330:RNA, Double-Stranded / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014774:Virulence; D014779:Virus Replication
D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D016899:Interferon-beta