[Diffuse lamellar keratitis: prophylactic treatment with ketorolac tromethamine 0.5% in an animal model].
OBJECTIVE
The objective of this study is to evaluate the use of a topical non-steroidal anti-inflammatory drug in the treatment of induced diffuse lamellar keratitis (DLK) in an animal model.


METHODS
A corneal flap was created in 40 eyes of 20 Dutch-belted rabbits using the ASC microkeratome. The interface was inoculated with either Pseudomonas Aeruginosa Lipopolysaccharide (LPS) endotoxin or Ultra Palmolive liquid dish washer. The rabbits were divided in two groups: Group I (n=20) treated with ketorolac tromethamine ophthalmic solution 0.5% 4 times a day and the group II (n=20) used as control. The rabbits were examined at the slit lamp at day 1, 3, 5 and 7 postoperatively. DLK was graded from I-IV.


RESULTS
At the end of the study 31 eyes were available for evaluation. 28 eyes (90%) developed DLK: 86% of the treated group and 94% of the control group during the follow-up. The treated group showed a lower rate of DLK as well as a lower severity. However, no statistically significant difference was found when comparing both groups (P>0.05).


CONCLUSIONS
Pseudomonas aeruginosa LPS endotoxin and Palmolive Ultra can induce DLK in rabbit eyes. The postoperative prophylactic treatment with a topical non-steroidal anti-inflammatory drug showed a tendency towards a lower DLK rate as well as the severity of the disease.
Sandoval
H P
HP
Magill Research Center for Vision Correction, Storm Eye Institute, Medical University of South Carolina, SC 29425, USA. sandoval@musc.edu
Vargas
L G
LG
Holzer
M P
MP
Kasper
T J
TJ
Vroman
D T
DT
Apple
D J
DJ
Solomon
K D
KD
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D007634:Keratitis / Q000188:drug therapy* / Q000517:prevention & control; D020731:Keratomileusis, Laser In Situ; D020911:Ketorolac Tromethamine / Q000627:therapeutic use*; D023421:Models, Animal; D009883:Ophthalmic Solutions; D011817:Rabbits; D013524:Surgical Flaps
D000894:Anti-Inflammatory Agents, Non-Steroidal; D009883:Ophthalmic Solutions; D020911:Ketorolac Tromethamine