title: Effects of single and repeated restraint stresses on the expression of beta(1)-adrenoceptor mRNA in the rat hypothalamus and midbrain.
abstract: We examined the effects of single and repeated restraint stresses on the expression of beta(1)-adrenoceptor mRNA in the rat midbrain and hypothalamus using reverse transcriptase-polymerase chain reaction (RT-PCR). After the rats had been restrained for 4 h (single stress), or for 4 h per day during 2 or 3 consecutive days, the hypothalamus and midbrain were removed immediately and beta(1)-adrenoceptor mRNA levels in these regions were determined by RT-PCR. Single stress significantly decreased the mRNA level in the hypothalamus, but the mRNA level was near control levels after 2 and 3 days of stress. In the midbrain, single stress had no effect on the mRNA level, but 2 days of stress significantly increased it.
authors.affiliation: Department of Psychiatry, Mie University School of Medicine, Tsu, Japan. Zhangkai@clin.medic.mie-u.ac.jp
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Teruhisa
authors.initials: T
authors.lastname: Komori
authors.forename: Satoru
authors.initials: S
authors.lastname: Miyahara
authors.forename: Masato
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Takuya
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Yuji
authors.initials: Y
authors.lastname: Okazaki
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D008636:Mesencephalon / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018342:Receptors, Adrenergic, beta-1 / Q000235:genetics*; D012149:Restraint, Physical / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013312:Stress, Physiological / Q000503:physiopathology*; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D018342:Receptors, Adrenergic, beta-1