title: Genomic and expression analysis of the 12p11-p12 amplicon using EST arrays identifies two novel amplified and overexpressed genes.
abstract: We performed parallel array comparative genomic hybridization and array expression analysis of the 12p11-p12 amplicon in human testicular seminomas and an ovarian carcinoma cell line using an expressed sequence tags (ESTs) array spotted with 8254 ESTs. The data were normalized using a robust statistical modeling and the significance inferred from the local SD. We identified two ESTs within the chromosomal amplicon that were amplified and overexpressed in > or =75-100 percent of analyzed tumors with the 12p11-p12 amplicon. These sequences, belonging to coding regions of two novel genes designated here as GCT1 and GCT2, were broadly expressed in a panel of human tissues, including testis and ovary. GCT1 and GCT2 were overexpressed in 92 and 71 percent, respectively, of a panel of seminomas tested. Combined array comparative genomic hybridization and array expression analysis is a valid approach for gene discovery in large chromosomal amplicons.
authors.affiliation: Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
authors.forename: Véronique
authors.initials: V
authors.lastname: Bourdon
authors.forename: Félix
authors.initials: F
authors.lastname: Naef
authors.forename: Pulivarthi H
authors.initials: PH
authors.lastname: Rao
authors.forename: Victor
authors.initials: V
authors.lastname: Reuter
authors.forename: Samuel C
authors.initials: SC
authors.lastname: Mok
authors.forename: George J
authors.initials: GJ
authors.lastname: Bosl
authors.forename: Sanjay
authors.initials: S
authors.lastname: Koul
authors.forename: Vundaralli V V S
authors.initials: VV
authors.lastname: Murty
authors.forename: Raju S
authors.initials: RS
authors.lastname: Kucherlapati
authors.forename: R S K
authors.initials: RS
authors.lastname: Chaganti
mesh_terms: D000368:Aged; D000465:Algorithms; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D020224:Expressed Sequence Tags; D005260:Female; D005784:Gene Amplification; D015870:Gene Expression; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D010051:Ovarian Neoplasms / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018239:Seminoma / Q000235:genetics*; D013736:Testicular Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured