Sows intramammarily inoculated with Escherichia coli at parturition. II. Effects on the densities of MHC class II(+), CD4(+) and CD8(+) cells in the mammary gland.
The aim of this study was to determine the density of MHC class II, CD4 and CD8 positive cells in mammary glands of sows around parturition, and whether the densities were altered following intramammary inoculation with Escherichia coli prior to parturition. Also, animals developing clinical disease after inoculation were compared with animals not developing clinical disease. Fourteen cross-bred primiparous sows were subject to intramammary inoculation with E. coli bacteria 24h before estimated parturition. Mammary gland biopsies were collected and clinical observations were made. Four sows were categorised as clinically ill based on general condition, body temperature and gross mammary affection. There were no changes in density of MHC class II, CD4 and CD8 positive cells in non-inoculated glands around parturition, while significant changes in densities were shown in inoculated glands. Here, the density of MHC class II, CD4 and CD8 positive cells reached a peak at 72 h post-inoculation (p<0.01). In sows developing clinical disease, there was a tendency to an over all lower density (p=0.07) of MHC class II positive cells in inoculated glands compared with sows not developing clinical disease. When comparing the categories with respect to the density of CD4 and CD8 positive cells, the sows developing clinical disease showed a higher density (p=0.03) of CD4 and CD8 positive cells in inoculated glands than sows not developing disease. No differences were shown between categories in non-inoculated glands. It is concluded that the density of MHC class II, CD4 and CD8 positive cells seems to be unaltered around parturition. However, there is a rapid increase in density of these cells following intramammary inoculation with E. coli. Also, the data suggest that there is a difference between sows developing and sows not developing clinical disease after inoculation with respect to the increase in density of MHC class II, CD4 and CD8 positive cells in the mammary gland.
Löving
M
M
Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Centre for Reproductive Biology in Uppsala (CRU), P O Box 7039, SLU, SE-75007, Uppsala, Sweden. malin.loving@svdhv.org
Magnusson
U
U
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology*; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D018655:Lymphocyte Count; D008321:Mammary Glands, Animal / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D008413:Mastitis / Q000276:immunology* / Q000662:veterinary; D036801:Parturition; D011247:Pregnancy; D013552:Swine; D013553:Swine Diseases / Q000276:immunology; D013997:Time Factors
D000949:Histocompatibility Antigens Class II