title: Dynamic changes of TNF-alpha content in dogs after gunshot wound in the limbs in hot and humid environment.
abstract: OBJECTIVE: To investigate the dynamic changes of TNF-alpha content in the plasma and body tissues of dogs after gunshot wound in the limbs in hot and humid environment. METHODS: Eighteen dogs with gunshot wound were divided into 3 groups (6 in each), one observed in normal environment (Ne group) and the other two in hot and humid environment including a heat-acclimatized group (HA group) and a non-acclimatied group (NHA group). The contents of tumor necrosis factor-alpha (TNF-alpha) in the plasma and muscle tissues from the gunshot wound tract were measured at 0, 1, 3, 4, 6, 8, 10, 14, 18, 24 h respectively after the injury. RESULTS: TNF-alpha content in the muscular tissue from gunshot wound tract was significantly higher than that in the plasma (P<0.05). At 4 h after injury, TNF-alpha content started to rise in NE group, reaching the peak level at 14 h, the rise and peaking of which occurred at 3 and 10 h respectively in NHE group with higher peak level than that in NE group (P<0.05). The changes of TNF-alpha participates in the pathophysiological process after gunshot wound in hot and humid environment and may play the role of and indicator for the pathological changes that take place after the injury.
authors.affiliation: Department of Orthopedics and Traumatology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Xu-Hui
authors.initials: XH
authors.lastname: Zhang
authors.forename: Guo-Xian
authors.initials: GX
authors.lastname: Pei
authors.forename: Kuan-Hai
authors.initials: KH
authors.lastname: Wei
authors.forename: Xiang-Ji
authors.initials: XJ
authors.lastname: Zhou