title: DNA and PNA sensing on mercury and carbon electrodes by using methylene blue as an electrochemical label.
abstract: Described here are the electrochemical parameters for MB on binding to DNA at hanging mercury drop electrode (HMDE), glassy carbon electrode (GCE), and carbon paste electrode (CPE) in the solution and at the electrode surface. MB, which interacts with the immobilized calf thymus DNA, was detected by using single-stranded DNA-modified HMDE or CPE (ssDNA-modified HMDE or CPE), bare HMDE or CPE, and double-stranded DNA-modified HMDE or CPE (dsDNA-modified HMDE or CPE) in combination with adsorptive transfer stripping voltammetry (AdTSV), differential pulse voltammetry (DPV), and alternating current voltammetry (ACV) techniques. The structural conformation of DNA and hybridization between synthetic peptide nucleic acid (PNA) and DNA oligonucleotides were determined by the changes in the voltammetric peak of MB. The PNA and DNA probes were also challenged with excessive and equal amount of noncomplementary DNA and a mixture that contained one-base mismatched and target DNA. The partition coefficient was also obtained from the signal of MB with probe, hybrid, and ssDNA-modified GCEs. The effect of probe, target, and ssDNA concentration upon the MB signal was investigated. These results demonstrated that MB could be used as an effective electroactive hybridization indicator for DNA biosensors. Performance characteristics of the sensor are described, along with future prospects.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey.
authors.forename: Dilsat
authors.initials: D
authors.lastname: Ozkan
authors.forename: Pinar
authors.initials: P
authors.lastname: Kara
authors.forename: Kagan
authors.initials: K
authors.lastname: Kerman
authors.forename: Burcu
authors.initials: B
authors.lastname: Meric
authors.forename: Arzum
authors.initials: A
authors.lastname: Erdem
authors.forename: Frantisek
authors.initials: F
authors.lastname: Jelen
authors.forename: Peter E
authors.initials: PE
authors.lastname: Nielsen
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ozsoz
mesh_terms: D000818:Animals; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D002244:Carbon / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D005898:Glass; D008628:Mercury / Q000737:chemistry*; D008751:Methylene Blue / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000032:analysis* / Q000737:chemistry; D017354:Point Mutation
chemical_list: D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D020135:Peptide Nucleic Acids; D002244:Carbon; D004247:DNA; C427203:calf thymus DNA; D008628:Mercury; D008751:Methylene Blue