title: Isolation and partial characterization of an antibacterial substance produced by Enterococcus faecium.
abstract: A strain of Enterococcus faecium isolated from Bulgarian yellow cheese "kashkaval" produced a bacteriocin-like substance named enterococcin A 2000. The antibacterial substance had a low molar mass (< 2 kDa), was relatively stable toward heat but was sensitive to selected proteolytic enzymes. It was active against Gram-positive bacteria including enterococci, such as Listeria, Bacillus and Streptococcus, and also against Gram-negative E. coli. Production of enterococcin A 2000 has a maximum near the end of the exponential phase of producer growth. The peptide was purified by ammonium sulfate precipitation, butanol extraction, followed by cation-exchange chromatography and reversed-phase chromatography. A partial sequence of purified enterococcin A 2000 indicated that this substance does not belong to the class IIa of bacteriocins presenting the consensus anti-Listeria motif YGNGV.
authors.affiliation: Department of Microbiology, Faculty of Biology, University of Sofia, 1423 Sofia, Bulgaria.
authors.forename: A
authors.initials: A
authors.lastname: Pantev
authors.forename: P
authors.initials: P
authors.lastname: Kabadjova
authors.forename: M
authors.initials: M
authors.lastname: Dalgalarrondo
authors.forename: T
authors.initials: T
authors.lastname: Haertlé
authors.forename: I
authors.initials: I
authors.lastname: Ivanova
authors.forename: X
authors.initials: X
authors.lastname: Dousset
authors.forename: H
authors.initials: H
authors.lastname: Prévost
authors.forename: J M
authors.initials: JM
authors.lastname: Chobert
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001430:Bacteriocins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D001483:Base Sequence; D002611:Cheese / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D016984:Enterococcus faecium / Q000737:chemistry* / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects; D008087:Listeria / Q000187:drug effects; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics
chemical_list: D000596:Amino Acids; D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S