Mapping of the active site of rat kidney gamma-glutamyl transpeptidase using activated esters and their amide derivatives.
The enzyme gamma-glutamyl transpeptidase (GGT), implicated in many physiological processes, catalyses the transfer of a gamma-glutamyl from a donor substrate to an acyl acceptor substrate, usually an amino acid or a peptide. In order to investigate which moieties of the donor substrate are necessary for recognition by GGT, the structure of the well-recognized substrate L-gamma-glutamyl-p-nitroanilide was modified. Several activated esters and their amide derivatives were synthesized and used as substrates. Kinetic (K(m) and V(max)) and inhibition constants (K(i)) were measured and reveal that almost the entire gamma-glutamyl moiety is necessary for recognition in the binding site of the donor substrate. The implied presence of certain complementary amino acids in this substrate binding site will allow the more rational design of various substrate analogues and inhibitors.
Castonguay
Roselyne
R
Département de chimie, Université de Montréal, C.P. 6128, Succursale Centre-Ville, Québec, Canada.
Lherbet
Christian
C
Keillor
Jeffrey W
JW
D000577:Amides / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D004952:Esters / Q000737:chemistry* / Q000494:pharmacology; D005973:Glutamine / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D010449:Peptide Mapping; D010648:Phenylacetates / Q000737:chemistry; D051381:Rats; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D005723:gamma-Glutamyltransferase / Q000737:chemistry* / Q000378:metabolism
D000577:Amides; D004791:Enzyme Inhibitors; D004952:Esters; D010648:Phenylacetates; D005973:Glutamine; D005723:gamma-Glutamyltransferase; C118008:4-nitrophenylacetic acid