title: Inhibitory effect of stevioside on tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in two-stage carcinogenesis in mouse skin.
abstract: Four steviol (ent-kaurene-type diterpenoid) glycosides, stevioside, rebaudiosides A and C, and dulcoside A, have been isolated from Stevia rebaudiana BERTONI. These compounds showed strong inhibitory activity against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice. The 50% inhibitory dose of these compounds for TPA-induced inflammation was 54.1-291.6 micro g/ear. Furthermore, at 1.0 and 0.1 mg/mouse of stevioside mixture, the mixture of these compounds markedly inhibited the promoting effect of TPA (1 micro g/mouse) on skin tumor formation initiated with 7,12-dimethylbenz[a]anthracene (50 micro g/mouse).
authors.affiliation: College of Pharmacy, Nihon University, Chiba, Japan. yasukawa@pha.nihon-u.ac.jp
authors.forename: Ken
authors.initials: K
authors.lastname: Yasukawa
authors.forename: Susumu
authors.initials: S
authors.lastname: Kitanaka
authors.forename: Shujiro
authors.initials: S
authors.lastname: Seo
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D045786:Diterpenes, Kaurane; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005260:Female; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D008813:Mice, Inbred ICR; D012878:Skin Neoplasms / Q000139:chemically induced / Q000188:drug therapy*; D036625:Stevia / Q000737:chemistry*; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors* / Q000633:toxicity*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D045786:Diterpenes, Kaurane; D005960:Glucosides; C012043:stevioside; D013755:Tetradecanoylphorbol Acetate