Antigen levels of the urokinase-type plasminogen activator and its gene polymorphisms in colorectal cancer.
We analysed the distribution of genotypes of two polymorphisms in the urokinase-type plasminogen activator (uPA) gene: C-->T substitution in exon 6 and T-->C substitution in intron 7 in 52 subjects with colorectal cancer. Genotypes were determined in tumour tissue and distant mucosa samples by allele-specific polymerase chain reaction. The antigen levels of uPA in cancer tissue were higher than in distant mucosa as measured by enzyme-linked immunosorbent assay. The level of uPA antigens in cancer samples with the C/C genotype of C-->T polymorphism in exon 6 was higher than in samples with C/T and T/T genotypes. No differences in the level of uPA antigens between the alleles of the intron 7 T-->C polymorphism were found. As uPA can be involved in cancer invasion and metastasis, C/C genotype in exon 6 of uPA gene can be further considered as being related to colorectal cancer progression.
Przybyłowska
K
K
Department of Molecular Genetics, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
Smolarczyk
K
K
Kulig
A
A
Romanowicz-Makowska
H
H
Dziki
A
A
Ulańska
J
J
Pander
B
B
Błasiak
J
J
D000368:Aged; D000941:Antigens / Q000032:analysis; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics* / Q000276:immunology*
D000941:Antigens; D014568:Urokinase-Type Plasminogen Activator