title: Susceptibility of Candida albicans isolates from the oral cavities of HIV-positive patients to histatin-5.
abstract: BACKGROUND
  Oral surfaces, including the denture-fitting surface, may serve as a reservoir for disseminated candidal infections, particularly in immunocompromised hosts such as patients with AIDS. Histatins are a group of small, cationic antifungal peptides present in human saliva. There is limited information on the antifungal activity of peptides against Candida albicans isolates from HIV-positive patients.
  
  
  OBJECTIVE
  This study investigated the fungicidal effects of histatin-5 against oral isolates of C. albicans from HIV-positive and HIV-negative patients.
  
  
  METHODS
  An isolate of C. albicans from each of 2 HIV-positive patients (both male) and 3 HIV-negative patients (2 male and 1 female) was obtained. American Type Culture Collection 90028 served as a reference strain. All isolates were identified with sugar assimilation tests and the germ tube test. Fungicidal assays were performed on exponential C. albicans cells in the presence or absence of 0.315 to 50 microm of histatin-5. Numerical data were subjected to 1-way analysis of variance and Tukey's multiple range test (P<.05).
  
  
  RESULTS
  Histatin-5 (50 microm) killed more than 95% of C. albicans isolates from HIV-negative patients and more than 90% of isolates from the reference strain. The same treatment induced 75.3% and 66.1% loss of viability in C. albicans isolates taken from HIV-positive patients (A1 and A2 cells, respectively). The difference between the fungicidal effects in the HIV-positive and HIV-negative groups was significant. (P<.05).
  
  
  CONCLUSIONS
  Within the limited population of this study, C. albicans isolates from the oral cavities of HIV-positive patients were less sensitive to histatin-5 than oral isolates from HIV-negative patients.
authors.affiliation: Department of Prosthetic Dentistry, Faculty of Dentistry, Hiroshima University, Hiroshima, Japan. hirocky@hiroshima-u.ac.jp
authors.forename: Hiroki
authors.initials: H
authors.lastname: Nikawa
authors.forename: Chen
authors.initials: C
authors.lastname: Jin
authors.forename: Seicho
authors.initials: S
authors.lastname: Makihira
authors.forename: Taizo
authors.initials: T
authors.lastname: Hamada
authors.forename: Lakshman P
authors.initials: LP
authors.lastname: Samaranayake
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000276:immunology / Q000382:microbiology*; D000704:Analysis of Variance; D000935:Antifungal Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects*; D002180:Candidiasis, Oral / Q000276:immunology* / Q000382:microbiology; D005260:Female; D018023:HIV Seronegativity; D006679:HIV Seropositivity / Q000382:microbiology; D054808:Histatins; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D012471:Salivary Proteins and Peptides / Q000008:administration & dosage / Q000494:pharmacology*; D018709:Statistics, Nonparametric
chemical_list: D000935:Antifungal Agents; C068075:HTN3 protein, human; D054808:Histatins; D012471:Salivary Proteins and Peptides