Simplified method for estimation of microbial activity in compost by ATP analysis.
A simplified method using ATP analysis was proposed to estimate the time course of microbial activity during composting. A compost sample was suspended in distilled water and its filtrate was used to estimate the ATP content in the compost by the luciferine-luciferase fluorescence reaction. The method permitted the rapid and simple determination of ATP in the compost and was successfully applied to monitor the time course of the microbial activity in the laboratory-scale composting process. The analytical simplicity in this method greatly improved the field-applicability of the ATP analysis for the composting process monitoring.
Horiuchi
J I
JI
Department of Chemical System Engineering, Kitami Institute of Technology, Kitami-shi, Hokkaido, Japan. horiuchi@betta.chem.kitami-it.ac.jp
Ebie
K
K
Tada
K
K
Kobayashi
M
M
Kanno
T
T
D000255:Adenosine Triphosphate / Q000032:analysis*; D000363:Agaricales / Q000254:growth & development; D000383:Agriculture / Q000379:methods; D007700:Kinetics; D008156:Luciferases / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012037:Refuse Disposal / Q000379:methods; D013997:Time Factors; D014934:Wood
D011994:Recombinant Proteins; D000255:Adenosine Triphosphate; D008156:Luciferases