IL-13 suppresses MUC5AC gene expression and mucin secretion in nasal epithelial cells.
Recently, it was found that IL-13 is involved in allergic inflammation and mucus hypersecretion in in vivo animal models. However, the role of IL-13 in in vitro cellular models has yet to be determined. This study aimed to investigate the direct effect of IL-13 on mucin gene expression and mucin secretion in cultured normal human nasal epithelial cells. After treatment with IL-13, total mucin and MUC5AC mucin levels were measured using an immunoblotting assay. MUC2, MUC5AC and MUC8 mRNA expression were determined using reverse transcription-polymerase chain reaction. Also, immunostaining was performed using a MUC5AC antibody on histologic and cytospin slides. After treatment with > or = 5 ng/ml IL-13, the level of total mucin and MUC5AC mucin secretion decreased substantially. The expression of MUC2 and MUC8 mRNA increased with higher concentrations of IL-13, but the expression of MUC5AC mRNA decreased. On the 7th day after IL-13 treatment, a significant decrease in the number of MUC5AC-positive cells was confirmed by immunostaining. These findings indicate that IL-13 suppresses MUC5AC mucin gene expression and mucin secretion in cultured normal human nasal epithelial cells in vitro.
Kim
Chang-Hoon
CH
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, South Korea.
Song
Kyoung Seob
KS
Koo
Ja Seok
JS
Kim
Hyun-Ung
HU
Cho
Jae Young
JY
Kim
Hyun Jun
HJ
Yoon
Joo-Heon
JH
D002452:Cell Count; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007150:Immunohistochemistry; D018793:Interleukin-13 / Q000494:pharmacology* / Q000502:physiology; D055271:Mucin 5AC; D055262:Mucin-2; D009077:Mucins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D009297:Nasal Mucosa / Q000166:cytology / Q000378:metabolism / Q000503:physiopathology*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018793:Interleukin-13; C524625:MUC2 protein, human; C524678:MUC5AC protein, human; C105059:MUC8 protein, human; D055271:Mucin 5AC; D055262:Mucin-2; D009077:Mucins; D012333:RNA, Messenger