title: Effects of a high-fat diet and strain on hypothalamic gene expression in rats.
abstract: OBJECTIVE
  This study was designed to investigate whether dietary fat and genetic background might differentially alter the expression of hypothalamic genes involved in food intake.
  
  
  METHODS
  Three-month-old Osborne-Mendel (OM) and S5B/Pl rats were fed either a high-fat or a low-fat diet for 14 days. mRNA for neuropeptide Y (NPY), corticotrophin-releasing hormone, NPY Y-1 receptor and Y-5 receptor, and serotonin 2c (5-HT2c) receptors were measured using Northern blotting or ribonuclease protection assays.
  
  
  RESULTS
  OM rats showed an increased expression of NPY and corticotrophin-releasing hormone compared with S5B/Pl rats. The expression of NPY-Y1 and -Y5 receptor mRNA was significantly higher in the hypothalamus of OM rats compared with S5B/Pl rats. The expression of 5HT-2c receptor mRNA was significantly reduced in both strains of rats eating a high-fat diet when compared with the animals eating the low-fat diet.
  
  
  CONCLUSIONS
  These data suggest that over activity of the NPY system may contribute to the development of obesity in OM rats and that expression of the 5HT-2c receptor gene may be modulated by dietary fat.
authors.affiliation: Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, Louisiana 70808, USA.
authors.forename: Andrea O
authors.initials: AO
authors.lastname: Schaffhauser
authors.forename: Abram M
authors.initials: AM
authors.lastname: Madiehe
authors.forename: Hugh D
authors.initials: HD
authors.lastname: Braymer
authors.forename: George A
authors.initials: GA
authors.lastname: Bray
authors.forename: David A
authors.initials: DA
authors.lastname: York
mesh_terms: D000818:Animals; D003345:Corticosterone / Q000097:blood; D003346:Corticotropin-Releasing Hormone / Q000235:genetics; D004041:Dietary Fats / Q000008:administration & dosage*; D004435:Eating; D015870:Gene Expression; D007031:Hypothalamus / Q000737:chemistry*; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D008297:Male; D009478:Neuropeptide Y / Q000235:genetics; D009765:Obesity / Q000235:genetics; D009929:Organ Size; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D044405:Receptor, Serotonin, 5-HT2C; D017476:Receptors, Neuropeptide Y / Q000235:genetics; D011985:Receptors, Serotonin / Q000235:genetics; D013045:Species Specificity; D015430:Weight Gain
chemical_list: D004041:Dietary Fats; D007328:Insulin; D020738:Leptin; D009478:Neuropeptide Y; D012333:RNA, Messenger; D044405:Receptor, Serotonin, 5-HT2C; D017476:Receptors, Neuropeptide Y; D011985:Receptors, Serotonin; C097212:neuropeptide Y-Y1 receptor; C100568:neuropeptide Y5 receptor; D003346:Corticotropin-Releasing Hormone; D003345:Corticosterone