23-Hydroxybetulinic acid-mediated apoptosis is accompanied by decreases in bcl-2 expression and telomerase activity in HL-60 Cells.
23-Hydroxybetulinic acid, a derivative of betulinic acid, was investigated for its apoptotic effect and the associated telomerase activity in human leukemia HL-60 cells. Apoptosis and bcl-2 were determined by flow cytometry analysis. A PCR-based telomeric repeat amplification protocol assay was used to detect telomerase activity. Results showed that 23-hydroxybetulinic acid induced growth arrest and apoptotic cell death in HL-60 cells. The apoptotic events were associated with concurrent down-regulation of bcl-2 and the telomerase activity. Our data suggest that 23-hydroxybetulinic acid may be a potential cytotoxic agent for treatment of cancer.
Ji
Zhao-Ning
ZN
Department of Pharmacology, China Pharmaceutical University, 210009, Nanjing, China
Ye
Wen-Cai
WC
Liu
Guo-Ging
GG
Hsiao
W L Wendy
WL
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000633:toxicity*; D017209:Apoptosis; D002455:Cell Division / Q000187:drug effects; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D018922:HL-60 Cells; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000276:immunology / Q000378:metabolism*; D019098:Telomerase / Q000378:metabolism*; D014315:Triterpenes / Q000737:chemistry / Q000633:toxicity*
C468651:23-hydroxybetulinic acid; D000972:Antineoplastic Agents, Phytogenic; D019253:Proto-Oncogene Proteins c-bcl-2; D014315:Triterpenes; D019098:Telomerase