Cloning, sequencing, and promoter identification of Blueberry red ringspot virus, a member of the family Caulimoviridae with similarities to the "Soybean chlorotic mottle-like" genus.
The double-stranded DNA genome of Blueberry red ringspot virus (BRRV), a member of the family Caulimoviridae, was cloned and sequenced. The genome organization and relationships of the 8303 nt sequence revealed BRRV to be a tentative member of the genus that has been provisionally named "Soybean chlorotic mottle-like viruses", rather than a member of the genus Caulimovirus, in which it had been placed previously. Insertion of the putative 35S promoter homolog of BRRV into promoterless constructs carrying the UidA (beta-glucuronidase) gene resulted in high-level transient expression from cranberry and stable expression from transgenic tobacco. Sequences of 5'-RACE clones derived from transcripts from transgenic tobacco were consistent with the map position of the promoter.
Glasheen
B M
BM
Department of Plant Biology and Pathology, Cook College, Rutgers University, New Brunswick, New Jersey 08901-8520, USA.
Polashock
J J
JJ
Lawrence
D M
DM
Gillett
J M
JM
Ramsdell
D C
DC
Vorsa
N
N
Hillman
B I
BI
D000595:Amino Acid Sequence; D001483:Base Sequence; D029798:Blueberry Plants / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics; D017796:Caulimovirus / Q000145:classification* / Q000235:genetics*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D013025:Glycine max / Q000821:virology*; D014158:Transcription, Genetic
D036022:Capsid Proteins; D004279:DNA, Viral