X-ray Crystal Structure of a Bisubstrate Inhibitor Bound to the Enzyme Catechol-O-methyltransferase: A Dramatic Effect of Inhibitor Preorganization on Binding Affinity We thank F. Hoffmann-La Roche for generous support of this work. We are grateful to P. Malherbe for the cloning of COMT, P. Caspers for the expression of COMT, A. Cesura for enzyme purification, B. Wipf for fermentation, and H. W. Lahm for sequencing.
Lerner
Christian
C
Laboratorium für Organische Chemie, ETH-Zentrum Universitätstrasse 16, 8092 Zürich (Switzerland).
Ruf
Armin
A
Gramlich
Volker
V
Masjost
Birgit
B
Zürcher
Gerhard
G
Jakob-Roetne
Roland
R
Borroni
Edilio
E
Diederich
François
F