title: Ischemic preconditioning attenuates the oxidant-dependent mechanisms of reperfusion cell damage and death in rat liver.
abstract: In an in vivo rat model of liver ischemia followed by reperfusion a consistent appearance of necrosis and activation of biochemical pathways of apoptosis was reproduced and monitored after 30 minutes reperfusion. Preconditioning by application of a short cycle of ischemia-reperfusion (10 minutes + 10 minutes) positively conditioned recovery of the organ at reperfusion, attenuating both necrotic and apoptotic events. Preconditioning at least halved cell oxidative damage occurring early at reperfusion, and as a major consequence, the increase of cytolysis and apoptosis occurring at reperfusion was about 50% less. The attenuation of both pathways of cell death by preconditioning appeared at least partly related to its modulate action on H(2)O(2) and 4-hydroxy-2,3-trans-nonenal production. The overall data point to a marked diminished oxidant generation and oxidative reactions as one major possible mechanism through which ischemic preconditioning exerts protection against necrotic and apoptotic insult to the postischemic liver.
authors.affiliation: Laboratory of Experimental Liver Pathology, the Department of Clinical and Biological Sciences, University of Turin, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Cavalieri
authors.forename: Maria-Giulia
authors.initials: MG
authors.lastname: Perrelli
authors.forename: Manuela
authors.initials: M
authors.lastname: Aragno
authors.forename: Raffaella
authors.initials: R
authors.lastname: Mastrocola
authors.forename: Giovanna
authors.initials: G
authors.lastname: Corvetti
authors.forename: Marilena
authors.initials: M
authors.lastname: Durazzo
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Poli
authors.forename: Juan C
authors.initials: JC
authors.lastname: Cutrìn
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D015536:Down-Regulation; D006861:Hydrogen Peroxide / Q000378:metabolism; D019194:Ischemic Preconditioning; D008099:Liver / Q000473:pathology* / Q000503:physiopathology*; D008102:Liver Circulation; D008297:Male; D009336:Necrosis; D016877:Oxidants / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology* / Q000503:physiopathology*
chemical_list: D000447:Aldehydes; D003574:Cytochrome c Group; D016877:Oxidants; C462359:tert-4-hydroxy-2-nonenal; D006861:Hydrogen Peroxide; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases