title: A Cajal body-specific pseudouridylation guide RNA is composed of two box H/ACA snoRNA-like domains.
abstract: Site-specific post-transcriptional conversion of uridines to pseudouridine in ribosomal RNAs and small nuclear RNAs (snRNAs) is directed by guide RNAs which possess the conserved box H and ACA sequence elements and fold into the consensus 'hairpin-hinge-hairpin-tail' secondary structure. Here, we describe an unusual mammalian pseudouridylation guide RNA, called U93, that is composed of two tandemly arranged box H/ACA RNA domains. The U93 RNA therefore carries two H and two ACA box motifs, all of which are essential for accumulation of the full-length RNA. The human U93 RNA accumulates in Cajal (coiled) bodies and it is predicted to function in pseudouridylation of the U2 spliceosomal snRNA. Our results lend further support to the notion that modification of the RNA polymerase II-transcribed spliceosomal snRNAs takes place in Cajal bodies.
authors.affiliation: Laboratoire de Biologie Moléculaire Eucaryote du CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex, France.
authors.forename: Arnold M
authors.initials: AM
authors.lastname: Kiss
authors.forename: Beáta E
authors.initials: BE
authors.lastname: Jády
authors.forename: Xavier
authors.initials: X
authors.lastname: Darzacq
authors.forename: Céline
authors.initials: C
authors.lastname: Verheggen
authors.forename: Edouard
authors.initials: E
authors.lastname: Bertrand
authors.forename: Tamás
authors.initials: T
authors.lastname: Kiss
mesh_terms: D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002347:Carps / Q000235:genetics; D002417:Cattle; D020541:Coiled Bodies / Q000235:genetics* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011560:Pseudouridine / Q000378:metabolism*; D012342:RNA, Small Nuclear / Q000235:genetics / Q000378:metabolism; D020537:RNA, Small Nucleolar / Q000737:chemistry* / Q000235:genetics; D020718:Ribonucleoproteins, Small Nucleolar / Q000235:genetics / Q000378:metabolism; D017381:Spliceosomes / Q000378:metabolism; D058727:RNA, Small Untranslated
chemical_list: C485802:GAR1 protein, human; C407731:GAR1 protein, mammalian; D012342:RNA, Small Nuclear; D020537:RNA, Small Nucleolar; D020718:Ribonucleoproteins, Small Nucleolar; C405484:U2 small nuclear RNA; D011560:Pseudouridine; D058727:RNA, Small Untranslated