title: X-ray Crystal Structure of a Bisubstrate Inhibitor Bound to the Enzyme Catechol-O-methyltransferase: A Dramatic Effect of Inhibitor Preorganization on Binding Affinity We thank F. Hoffmann-La Roche for generous support of this work. We are grateful to P. Malherbe for the cloning of COMT, P. Caspers for the expression of COMT, A. Cesura for enzyme purification, B. Wipf for fermentation, and H. W. Lahm for sequencing.
authors.affiliation: Laboratorium für Organische Chemie, ETH-Zentrum Universitätstrasse 16, 8092 Zürich (Switzerland).
authors.forename: Christian
authors.initials: C
authors.lastname: Lerner
authors.forename: Armin
authors.initials: A
authors.lastname: Ruf
authors.forename: Volker
authors.initials: V
authors.lastname: Gramlich
authors.forename: Birgit
authors.initials: B
authors.lastname: Masjost
authors.forename: Gerhard
authors.initials: G
authors.lastname: Zürcher
authors.forename: Roland
authors.initials: R
authors.lastname: Jakob-Roetne
authors.forename: Edilio
authors.initials: E
authors.lastname: Borroni
authors.forename: François
authors.initials: F
authors.lastname: Diederich