Behavior of phosphatase isoforms during sclerotium formation in Physarum polycephalum.
The behavior of phosphatase isoforms under dark-starvation from plasmodium of Physarum polycephalum were investigated to determine their possible roles in sclerotium formation. Two and a half days after dark-starvation, approximately 95% of plasmodia plates formed sclerotia. Specific phosphatase activity increased markedly up to ca. two-fold within the first day of starvation, after which the enzymatic activity decreased rapidly to a level less than the initial level within 2 days of the starvation period. Among the two isoforms of enzyme detected just before sclerotization under dark-starvation conditions, the enzymatic activity of the major isoform (Rm value of 0.6) decreased gradually within 1.5 days of starvation, then linearly to less than 20% of that at the beginning of the observation. Those of other major isoform (Rm value of 0.7) increased up to ca. two-fold within the first day of starvation, then decreased linearly to levels less than that of the first 2 days of the starvation period. Behavior of this isoform strongly suggests that it initiates the formation of sclerotium under dark-starvation conditions.
Tanaka
Yuhko
Y
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, 2-8-1-Mejirodai, Bunkyo-ku, Tokyo 112-8681, Japan.
Suzuki
Yohko
Y
Osumi
Masako
M
Kaneko
Takako S
TS
D000818:Animals; D003624:Darkness; D008027:Light; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D016839:Physarum polycephalum / Q000201:enzymology* / Q000254:growth & development* / Q000648:ultrastructure; D020033:Protein Isoforms / Q000378:metabolism; D013997:Time Factors
D020033:Protein Isoforms; D010744:Phosphoric Monoester Hydrolases