title: NPY modulates epinephrine-induced leukocytosis via Y-1 and Y-5 receptor activation in vivo: sympathetic co-transmission during leukocyte mobilization.
abstract: Sympathetic nervous system (SNS) activation mobilizes blood leukocytes. Under these circumstances, both epinephrine (EPI) and neuropeptide Y (NPY) are released. Therefore, we investigated a possible interaction between these transmitters during leukocyte mobilization, using intravenous catheterization of male adult Lewis rats. Intravenous application of NPY followed by EPI, dose-dependently facilitated, intensified and inhibited EPI-induced leukocytosis with subset-specificity for NK-cells, monocytes, and B-lymphocytes. Pharmacological assessment of NPY receptors involved revealed a Y-1R-mediated inhibition and a Y-5R-mediated facilitation. RT-PCR on peripheral blood mononuclear cells (PBMC) detected Y-1R mRNA only, suggesting direct Y-1R-mediated effects on leukocytes and indirect effects via the Y-5R. Thus, via a specific Y-1R/Y-5R interplay, NPY acts as a neuroimmune co-transmitter in vivo.
authors.affiliation: Department of Functional and Applied Anatomy, Hannover Medical School, 30625 Hanover, Germany.
authors.forename: Sammy
authors.initials: S
authors.lastname: Bedoui
authors.forename: Sandra
authors.initials: S
authors.lastname: Lechner
authors.forename: Thomas
authors.initials: T
authors.lastname: Gebhardt
authors.forename: Heike
authors.initials: H
authors.lastname: Nave
authors.forename: Annette G
authors.initials: AG
authors.lastname: Beck-Sickinger
authors.forename: Rainer H
authors.initials: RH
authors.lastname: Straub
authors.forename: Reinhard
authors.initials: R
authors.lastname: Pabst
authors.forename: Stephan
authors.initials: S
authors.lastname: von Hörsten
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects*; D007964:Leukocytosis / Q000139:chemically induced*; D008297:Male; D009478:Neuropeptide Y / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011917:Rats, Inbred Lew; D017476:Receptors, Neuropeptide Y / Q000235:genetics / Q000502:physiology*; D009435:Synaptic Transmission
chemical_list: D009478:Neuropeptide Y; D012333:RNA, Messenger; D017476:Receptors, Neuropeptide Y; C097212:neuropeptide Y-Y1 receptor; C100568:neuropeptide Y5 receptor; D004837:Epinephrine