Approaches to library screening.
Fuelled by the drive to complete the Human Genome Project, many laboratories have developed new methods of screening clone libraries. From PCR-based strategies to pooling schemes and increased automation, the tedious task of library screening has become less labour-intensive and more cost-efficient. Currently, two main screening methods dominate: hybridization and polymerase chain reaction (PCR). In the following article, we present a brief overview of hybridization and PCR-based screening of yeast and bacterial libraries. Multi-faceted approaches combining different techniques, as well as less frequently employed methods such as fingerprinting are also described.
Campbell
Tessa N
TN
Center for Biomedical Research, Department of Biology, University of Victoria, British Columbia, Canada. tessac@uvic.ca
Choy
Francis Y M
FY
D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods; D015698:Genomic Library; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*
D004247:DNA