Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes.
We have developed a rapid and easy to perform fluorescence in situ hybridization test that allows specific identification of trypanosomes from the subgenus Trypanozoon, using peptide nucleic acid probes. Probes were designed to target subgenus-specific sequences on the multiple-copy 18S rRNA, greatly facilitating the detection of a single trypanosome.
Radwanska
M
M
Department of Parasitology, Institute of Tropical Medicine, Antwerp, Belgium. mradwanska@uctgsh1.uct.ac.za
Magez
S
S
Perry-O'Keefe
H
H
Stender
H
H
Coull
J
J
Sternberg
J M
JM
Büscher
P
P
Hyldig-Nielsen
J J
JJ
D000818:Animals; D001769:Blood / Q000469:parasitology; D002417:Cattle; D004275:DNA, Ribosomal / Q000032:analysis; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D014345:Trypanosoma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014353:Trypanosomiasis, African / Q000469:parasitology / Q000662:veterinary
D004275:DNA, Ribosomal; D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids; D012337:RNA, Ribosomal, 18S