[Effect of interleukin-1beta on aromatase activity in human osteoblast-like cells].
OBJECTIVE
To study the stimulation of interleukin (IL)-1beta and interleukin-1 receptor antagonist (IL-1ra) on aromatase activity in human osteoblast cells.


METHODS
In the present study, the effect of IL-1beta and IL-1ra on aromatase (Arom) activity and mRNA expression in human osteoblast-like cells was investigated by [(3)H] water released upon the conversion of [1beta-(3)H] androstenedione to estrone and the reverse-transcription polymerase cain reaction (RT-PCR) method. The experimental group is divided into four group: G1 (IL-1beta 1 microgram/L), G2 (10 microgram/L), G3 (IL-1beta 10 microgram/L + IL-1ra 500 microgram/L) and G4 (IL-1ra 500 microgram,/L).


RESULTS
The activity of G1 and G2 resulted in (4 211 +/- 348) pmol/10(6) cell.h(-1) and (4 958 +/- 231) pmol/10(6) cell.h(-1), respectively. IL-1ra neutralized the increased arom activity to control level in G3 (3 652 +/- 203) pmol/10(6) cell.h(-1). Arom mRNA expression was increased with the dose increasing of IL-1beta.


CONCLUSIONS
These findings suggest that IL-1beta stimulates arom activity through the IL-1 receptor and addition of IL-1beta increased arom activity in a dose-dependent manner.
Ma
Xiaoxin
X
Department of Obstetrics and Gynecology, Second College of China Medical University, Shenyang 110004, China.
Li
Yan
Y
Zhang
Shulan
S
Saito
H
H
Yanaihara
T
T
D001141:Aromatase / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D008875:Middle Aged; D010006:Osteoblasts / Q000187:drug effects / Q000201:enzymology*; D017698:Postmenopause; D012333:RNA, Messenger / Q000032:analysis
D007375:Interleukin-1; D012333:RNA, Messenger; D001141:Aromatase