title: [Effect of interleukin-1beta on aromatase activity in human osteoblast-like cells].
abstract: OBJECTIVE
  To study the stimulation of interleukin (IL)-1beta and interleukin-1 receptor antagonist (IL-1ra) on aromatase activity in human osteoblast cells.
  
  
  METHODS
  In the present study, the effect of IL-1beta and IL-1ra on aromatase (Arom) activity and mRNA expression in human osteoblast-like cells was investigated by [(3)H] water released upon the conversion of [1beta-(3)H] androstenedione to estrone and the reverse-transcription polymerase cain reaction (RT-PCR) method. The experimental group is divided into four group: G1 (IL-1beta 1 microgram/L), G2 (10 microgram/L), G3 (IL-1beta 10 microgram/L + IL-1ra 500 microgram/L) and G4 (IL-1ra 500 microgram,/L).
  
  
  RESULTS
  The activity of G1 and G2 resulted in (4 211 +/- 348) pmol/10(6) cell.h(-1) and (4 958 +/- 231) pmol/10(6) cell.h(-1), respectively. IL-1ra neutralized the increased arom activity to control level in G3 (3 652 +/- 203) pmol/10(6) cell.h(-1). Arom mRNA expression was increased with the dose increasing of IL-1beta.
  
  
  CONCLUSIONS
  These findings suggest that IL-1beta stimulates arom activity through the IL-1 receptor and addition of IL-1beta increased arom activity in a dose-dependent manner.
authors.affiliation: Department of Obstetrics and Gynecology, Second College of China Medical University, Shenyang 110004, China.
authors.forename: Xiaoxin
authors.initials: X
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Shulan
authors.initials: S
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Yanaihara
mesh_terms: D001141:Aromatase / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D008875:Middle Aged; D010006:Osteoblasts / Q000187:drug effects / Q000201:enzymology*; D017698:Postmenopause; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D001141:Aromatase