Array technology as a tool to monitor exposure of fish to xenoestrogens.
A variety of anthropogenic chemicals are capable of binding to the estrogen receptor of vertebrate species. Binding of these compounds can interfere with homeostasis by disrupting normal gene expression patterns. The purpose of this study was to investigate the feasibility of applying array technology as a monitoring tool for detecting the presence and distribution of estrogenic compounds in coastal habitats using sheepshead minnows as our model. cDNA clones that were isolated from differential display, including vitellogenin alpha and beta, vitelline envelope protein (ZP2), and transferrin, among others, were spotted on the macroarray. The results of these experiments demonstrate a characteristic expression pattern of estrogen responsive genes in sheepshead minnows exposed to 17 beta-estradiol (E2).
Larkin
Patrick
P
Department of Biochemistry and Molecular Biology and Center for Biotechnology, Gainesville, FL 32610 USA.
Folmar
Leroy C
LC
Hemmer
Michael J
MJ
Poston
Arianna J
AJ
Lee
H Stephen
HS
Denslow
Nancy D
ND
D000818:Animals; D003530:Cyprinidae / Q000235:genetics / Q000502:physiology; D004527:Egg Proteins / Q000096:biosynthesis; D004703:Endocrine System / Q000187:drug effects; D004781:Environmental Exposure; D004784:Environmental Monitoring; D004968:Estrogens, Non-Steroidal / Q000009:adverse effects*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005819:Genetic Markers; D020411:Oligonucleotide Array Sequence Analysis; D011960:Receptors, Estrogen / Q000187:drug effects*; D014168:Transferrin / Q000096:biosynthesis; D014819:Vitellogenins / Q000096:biosynthesis; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
D004527:Egg Proteins; D004968:Estrogens, Non-Steroidal; D005819:Genetic Markers; D011960:Receptors, Estrogen; D014168:Transferrin; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; C035651:vitelline membrane proteins