Real-time fluorescence-based PCR for detection of malaria parasites.
A fluorescence-based real-time 5' nuclease PCR capable of detecting all four human malaria parasites was developed to screen large numbers of samples during an outbreak to prevent further transmission of malaria. The effectiveness of antimalarial therapy for malaria patients can be monitored by determining the reduction of parasitemia by this method.
Lee
May-Ann
MA
Defence Medical Research Institute, Defence Science and Technology Agency, National University of Singapore, Singapore 117597. nmiv13@nus.edu.sg
Tan
Chiew-Hoon
CH
Aw
Lay-Tin
LT
Tang
Choon-Siang
CS
Singh
Mulkit
M
Lee
Sze-Hee
SH
Chia
Hwee-Pin
HP
Yap
Eric P H
EP
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis; D003851:Deoxyribonucleases / Q000378:metabolism*; D006801:Humans; D008288:Malaria / Q000469:parasitology*; D018512:Parasitemia / Q000469:parasitology; D010961:Plasmodium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D019914:Taq Polymerase / Q000378:metabolism
D016054:DNA, Protozoan; D019914:Taq Polymerase; D003851:Deoxyribonucleases