Herpetomonas samuelpessoai: dimethylsulfoxide-induced differentiation is influenced by proteinase expression.
In this study, we analyzed the influence of proteinase expression on the cellular differentiation of Herpetomonas samuelpessoai. Along cellular differentiation, which was induced by dimethylsulfoxide (DMSO), the trypanosomatids secreted several molecules with variable proteolytic activity. All of them were inhibited by 10 m M 1,10-phenanthroline, suggesting that they are zinc-metalloproteinases. Analysis of parasite extracts revealed the occurrence of a 63-kDa metalloproteinase and a 45-kDa cysteine proteinase. After extraction with Triton X-114 followed by water-detergent partition, the 63-kDa component was present in both aqueous and detergent phases, which indicated that this enzyme may be distributed over different cellular compartments including membrane domains. The 45-kDa component, however, presented hydrophilic properties and was predominantly expressed by DMSO non-treated parasites, suggesting that proteinases may be involved in the process of cellular differentiation in H. samuelpessoai. This was confirmed by the fact that a cysteine proteinase inhibitor abrogated parasite differentiation. The role of proteinases and their relevance in the differentiation of H. samuelpessoai are discussed.
Souza Dos Santos
André Luis
AL
Departamento de Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes (IMPPG), Centro de Ciências da Saúde (CCS), Bloco I, Universidade do Brasil (UFRJ), Ilha do Fundão, 21941-590, Rio de Janeiro, RJ, Brazil.
Rodrigues
Marcio Lourenço
ML
Alviano
Celuta Sales
CS
Angluster
Jayme
J
De Araújo Soares
Rosangela Maria
RM
D000818:Animals; D002454:Cell Differentiation; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008666:Metalloendopeptidases / Q000032:analysis / Q000378:metabolism*; D014351:Trypanosomatina / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development
D008666:Metalloendopeptidases; D004121:Dimethyl Sulfoxide