title: Differential expression patterns of secreted frizzled related protein genes in synovial cells from patients with arthritis.
abstract: OBJECTIVE
  To detect expression of the secreted frizzled related protein (sFRP) gene in synovial cells from patients with arthritis.
  
  
  METHODS
  Expression of sFRP-1, 2, 3, 4, and 5 genes was detected in synovial cells from patients with rheumatoid arthritis (RA) and osteoarthritis (OA) by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. To identify synovial cell populations expressing sFRP-1, 3, and 4 genes, expression was compared in macrophage-rich populations and fibroblast-like cell-rich populations by RT-PCR. Levels of expression of these genes were also studied in activated peripheral blood mononuclear cells (PBMC) and activated skin fibroblasts.
  
  
  RESULTS
  Expression of the sFRP-1, 3, and 4 genes was observed in both RA and OA synovial cells. sFRP-1 and 4 genes were expressed predominantly in fibroblast-like cell-rich populations, and the sFRP-3 gene was expressed predominantly in macrophage-rich populations. Levels of expression of sFRP-3 and 4 genes were elevated in activated PBMC and activated skin fibroblasts.
  
  
  CONCLUSIONS
  Our findings suggest that sFRP-1, 3, and 4 may play different roles in the pathogenesis of synovitis.
authors.affiliation: Department of Orthopaedic Surgery, Kagoshima University, Kagoshima, Japan.
authors.forename: Kosei
authors.initials: K
authors.lastname: Ijiri
authors.forename: Ryusaku
authors.initials: R
authors.lastname: Nagayoshi
authors.forename: Noriko
authors.initials: N
authors.lastname: Matsushita
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Tsuruga
authors.forename: Noboru
authors.initials: N
authors.lastname: Taniguchi
authors.forename: Akira
authors.initials: A
authors.lastname: Gushi
authors.forename: Harutoshi
authors.initials: H
authors.lastname: Sakakima
authors.forename: Setsuro
authors.initials: S
authors.lastname: Komiya
authors.forename: Takami
authors.initials: T
authors.lastname: Matsuyama
mesh_terms: D001168:Arthritis / Q000235:genetics* / Q000378:metabolism* / Q000503:physiopathology; D001172:Arthritis, Rheumatoid / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008264:Macrophages / Q000378:metabolism; D010003:Osteoarthritis / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D011506:Proteins / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D013583:Synovial Membrane / Q000378:metabolism* / Q000503:physiopathology; D051153:Wnt Proteins; D029961:Zebrafish Proteins; D000099274:Frizzled-Related Proteins; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D051153:Wnt Proteins; D029961:Zebrafish Proteins; D000099274:Frizzled-Related Proteins; D000094742:Secreted Frizzled-Related Proteins