Glyceride stearic acid content and structure affect the energy available to growing rats.
To better understand the relative absorption of 18:0, specific structured triglycerides (STG) with varied ratios of 18:0 and short-chain organic acids (2:0, 3:0, 4:0) were compared with naturally occurring 18:0 in cocoa butter and to other mono- and diglycerides (DGs) containing 18:0. A bioassay for available fat energy was developed for growing Sprague-Dawley rats fed reduced energy from a control diet containing an American Heart Association (AHA) fat blend to generate 60 or 80% normal growth. The resulting standard growth curve was applied to the test fats, including cocoa butter and six glycerides, which were blended 3:1 with the AHA blend (to ensure EFA sufficiency) and pair-fed to match intake of control rats (AHA diet, 80% normal growth). Available energy from test fats ranged from 30 to 12 kJ/g (7.1 to 2.9 kcal/g) for cocoa butter to 18:0-DG, respectively, with the mean of the four different STG being 22 kJ/g (5.2 kcal/g). Energy available from test fats was negatively related to total 18:0 in the STG (r = -0.90; P < 0.001) and fecal dry weight (r = -0.92; P < 0.001); the effect was greater for monoglyceride (monolong-18:0) than for DG (dilong-18:0) but was not related to fecal 18:0. Compared with monoglyceride-18:0, available energy was increased or decreased when short-chain organic acids (SCOA) were added to form triglycerides, depending on the addition of butyrate or acetate, respectively. The different fat sources altered the available energy without apparent changes in lipoproteins or body composition. Thus, the reduced energy available from a glyceride containing 18:0 is determined by its total 18:0 and reflects the mono- or dilong chain character of the glyceride, its content of SCOA and triglyceride structure or organization per se.
Treadwell
Robyn M
RM
Foster Biomedical Research Laboratory, Brandeis University, Waltham, MA 02254, USA.
Pronczuk
Andrzej
A
Hayes
K C
KC
D000818:Animals; D001682:Biological Availability; D002784:Cholesterol / Q000097:blood; D004041:Dietary Fats / Q000032:analysis / Q000493:pharmacokinetics*; D002149:Energy Intake; D004734:Energy Metabolism; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000737:chemistry; D005989:Glycerides / Q000032:analysis / Q000737:chemistry* / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013229:Stearic Acids / Q000032:analysis*; D014280:Triglycerides / Q000097:blood; D015430:Weight Gain
D004041:Dietary Fats; D005227:Fatty Acids; D005989:Glycerides; D013229:Stearic Acids; D014280:Triglycerides; C031183:stearic acid; D002784:Cholesterol