Isolation of bovine viral diarrhea virus from an alpaca.
A frozen, stillborn alpaca (Lama pacos) was submitted to the Minnesota Veterinary Diagnostic Laboratory for diagnostic purposes. No gross or histopathologic changes of any significance were seen. A pool of lung, liver, and brain tissues was positive for bovine viral diarrhea virus (BVDV) by reverse transcription-polymerase chain reaction. On inoculation in cell cultures, a noncytopathic BVDV (type 1b) was isolated. No evidence of BVDV was seen on immunohistochemical examination of tissues. This indicates the importance of using multiple tests for arriving at a diagnosis and appears to be the first report of BVDV isolation from alpaca.
Goyal
Sagar M
SM
Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
Bouljihad
Mostafa
M
Haugerud
Sigrun
S
Ridpath
Julia F
JF
D000818:Animals; D002161:Camelids, New World / Q000821:virology*; D001908:Diarrhea Viruses, Bovine Viral / Q000302:isolation & purification* / Q000472:pathogenicity; D005260:Female; D005313:Fetal Death / Q000662:veterinary; D007150:Immunohistochemistry; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction