Endosomal/lysosomal targeting of a single helper T-cell epitope of an intracellular bacterium by DNA immunisation induces a specific T-cell subset and partial protective immunity in vivo.
We evaluated here the effect of the intracellular targeting of a helper T-cell (Th) epitope, literiolysin O 215-226 derived from Listeria monocytogenes, on induction of a specific Th by gene gun immunisation. Immunisation of C3H/He mice with pE215LAMP plasmid encoding the Th epitope fused with the endosomal/lysosomal targeting signal of lysosome-associated membrane protein (LAMP)-1 gave the epitope-specific proliferative responses of CD4(+) T lymphocytes. In addition, specific interferon-gamma production from the splenocytes was observed. Concomitantly, pE215LAMP-immunised mice showed moderate, but significant protective immunity against listerial challenge. These results suggest that the intracellular targeting of a Th epitope to endosomal/lysosomal compartments by DNA immunisation is useful for eliciting a specific Th subset in vivo.
Uchiyama
Hiroshi
H
Second Department of Internal Medicine, Hamamatsu University School of Medicine, Hamamatsu, Japan.
Nagata
Toshi
T
Yamada
Takashi
T
Uchijima
Masato
M
Aoshi
Taiki
T
Suda
Takafumi
T
Chida
Kingo
K
Nakamura
Hirotoshi
H
Koide
Yukio
Y
D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology*; D002452:Cell Count; D002478:Cells, Cultured; D018984:Epitopes, T-Lymphocyte / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000276:immunology*; D006460:Hemolysin Proteins; D007111:Immunity, Cellular; D007114:Immunization; D007371:Interferon-gamma / Q000032:analysis; D008089:Listeria monocytogenes; D008088:Listeriosis / Q000382:microbiology / Q000517:prevention & control*; D051907:Lysosomal Membrane Proteins; D051379:Mice; D008809:Mice, Inbred C3H; D013154:Spleen / Q000166:cytology / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D019444:Vaccines, DNA / Q000008:administration & dosage*
D015703:Antigens, CD; D001427:Bacterial Toxins; D018984:Epitopes, T-Lymphocyte; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D051907:Lysosomal Membrane Proteins; D019444:Vaccines, DNA; D007371:Interferon-gamma; C038705:hlyA protein, Listeria monocytogenes