Isolation of glucocardiolipins from Geobacillus stearothermophilus NRS 2004/3a.
Glucose-substituted cardiolipins account for about 4 mol% of total phospholipid extracted from exponentially grown cells of Geobacillus stearothermophilus NRS 2004/3a. Individual glucocardiolipin species exhibited differences in fatty acid substitution, with iso-C(15:0) and anteiso-C(17:0) prevailing. The compounds were purified to homogeneity by a novel protocol and precharacterized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
Schäffer
Christina
C
Zentrum für Ultrastrukturforschung und Ludwig Boltzmann-Institut für Molekulare Nanotechnologie, Universität für Bodenkultur Wien, A-1180 Vienna, Austria. CRS@edv1.boku.ac.at
Beckedorf
Anke I
AI
Scheberl
Andrea
A
Zayni
Sonja
S
Peter-Katalinić
Jasna
J
Messner
Paul
P
D001406:Bacillaceae / Q000737:chemistry*; D002308:Cardiolipins / Q000032:analysis / Q000302:isolation & purification*; D005947:Glucose; D008562:Membrane Glycoproteins; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D002308:Cardiolipins; D008562:Membrane Glycoproteins; C055764:S-layer proteins; D005947:Glucose