title: Treatment with an anti-CD18 monoclonal antibody in rabbits inhibits pneumococcal-induced leukocyte recruitment in the skin, but not in the meninges.
abstract: Inflammatory recruitment of leukocytes into the cerebrospinal fluid (CSF) during bacterial meningitis has been shown to contribute to the neurological damage commonly associated with this disease. In this study we tested whether inhibition of firm leukocyte adhesion to vascular endothelium could reduce leukocyte recruitment into the subarachnoid space (SAS) and into the skin in rabbits challenged with pneumococcal cell wall (PCW) antigen. PCW was given either as an intracisternal or an intradermal (i.d.) injection. Intravenous (i.v.) treatment with a monoclonal antibody (mAb), IB4, against the leukocytic adhesion molecule CD18 has previously been documented to attenuate leukocyte CSF accumulation in experimental bacterial meningitis. In the present study, i.v. treatment with anti-CD18 mAbs (IB4) only tended to inhibit CSF leukocyte influx in animals with PCW-induced meningitis. However, if the antigen was injected i.d., treatment i.v. with the same mAb (IB4) dramatically reduced leukocyte accumulation in the skin. Our findings indicate that the mechanisms responsible for PCW-induced inflammatory accumulation of leukocytes in skin and meninges are different.
authors.affiliation: Department of Infectious Diseases, Karolinska Institute, I 73, Huddinge University Hospital, Sweden. carl.granert@medhs.ki.se
authors.forename: Carl
authors.initials: C
authors.lastname: Granert
authors.forename: Johan
authors.initials: J
authors.lastname: Raud
authors.forename: Lars
authors.initials: L
authors.lastname: Lindquist
authors.forename: Lennart
authors.initials: L
authors.lastname: Lindbom
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D018821:CD18 Antigens / Q000276:immunology*; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D008578:Meninges / Q000276:immunology; D008581:Meningitis / Q000276:immunology / Q000517:prevention & control*; D011008:Pneumococcal Infections / Q000276:immunology / Q000517:prevention & control*; D011134:Polysaccharides / Q000276:immunology / Q000494:pharmacology; D011817:Rabbits; D012867:Skin / Q000276:immunology; D012871:Skin Diseases / Q000276:immunology / Q000517:prevention & control*; D013296:Streptococcus pneumoniae / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity
chemical_list: D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D011134:Polysaccharides; C007789:fucoidan