title: Dual-tag prokaryotic vectors for enhanced expression of full-length recombinant proteins.
authors.affiliation: Clinical Research Institute of Montreal, University of Montreal, Que, H2W 1R7, Montreal, Canada.
authors.forename: Philippe
authors.initials: P
authors.lastname: Coulombe
authors.forename: Sylvain
authors.initials: S
authors.lastname: Meloche
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001709:Biotechnology / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D005821:Genetic Techniques; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics*; D048053:Mitogen-Activated Protein Kinase 6; D020928:Mitogen-Activated Protein Kinases / Q000737:chemistry; D008969:Molecular Sequence Data; D011387:Prokaryotic Cells; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase; D048053:Mitogen-Activated Protein Kinase 6; D020928:Mitogen-Activated Protein Kinases