In vitro mechanistic differences in benzo[a]pyrene-DNA adduct formation using fish liver and mussel digestive gland microsomal activating systems.
Turbot (Scophthalmus maximus) and mussel (Mytilus edulis) microsomes were incubated with DNA to examine if microsomal in vitro metabolism of BaP could result in DNA adducts detected by 32P-postlabelling. Turbot DNA was incubated with benzo[a]pyrene (BaP), NADPH and microsomal activating systems prepared from either livers of unexposed turbot, turbot exposed to BaP or beta-naphthoflavone (beta-NF) or digestive glands from mussels. The beta-NF activating system generated the highest levels of DNA adducts detected in this study (451.7 adducts per 10(8) nucleotides) and were distributed in three discrete adduct TLC spots, one of which (97% of the total adducts) co-migrated with the 32P-postlabelled BaP 7,8-diol, 9,10-epoxide-N2-guanine adduct. Fewer adducts (P < 0.05) were generated by BaP-induced microsomes (9.4-30.6 adducts per 108 nucleotides) but levels were higher (P <0.05) than those generated from untreated fish (3.5 adducts per 10(8) nucleotides). Co-incubation with 500 microM alpha-naphthoflavone (alpha-NF) resulted in 97-99% inhibition in adduct formation implicating cytochrome P450-dependent (CYP) bioactivation however there was some evidence for carry over of BaP in the liver microsomal preparations from BaP injected fish. In contrast to the fish activating systems, no DNA adducts were observed when mussel microsomes were incubated with BaP, DNA and NADPH.
Peters
L D
LD
NERC Plymouth Marine Laboratory, Devon, UK.
Telli-Karakoç
F
F
Hewer
A
A
Phillips
D H
DH
D000818:Animals; D001564:Benzo(a)pyrene / Q000009:adverse effects* / Q000494:pharmacology; D049872:Bivalvia / Q000235:genetics* / Q000502:physiology; D018929:Cell Culture Techniques; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000494:pharmacology; D018736:DNA Adducts; D004249:DNA Damage; D004064:Digestive System / Q000473:pathology; D005412:Flatfishes / Q000235:genetics* / Q000502:physiology; D008099:Liver / Q000473:pathology; D008861:Microsomes; D010761:Phosphorus Radioisotopes / Q000493:pharmacokinetics / Q000627:therapeutic use
D018736:DNA Adducts; D010761:Phosphorus Radioisotopes; D001564:Benzo(a)pyrene; D003577:Cytochrome P-450 Enzyme System