Collaborative evaluation of optimal antifungal susceptibility testing conditions for dermatophytes.
A multicenter study was conducted to define the most suitable testing conditions for antifungal susceptibility of dermatophytes. Broth microdilution MICs of clotrimazole, itraconazole, and terbinafine were determined in three centers against 60 strains of dermatophytes. The effects of inoculum density (ca. 10(3) and 10(4) CFU/ml), incubation time (3, 7, and 14 days), endpoint criteria for MIC determination (complete [MIC-0] and prominent [MIC-2] growth inhibition), and incubation temperature (28 and 37 degrees C) on intra- and interlaboratory agreement were analyzed. The optimal testing conditions identified were an inoculum of 10(4) CFU/ml, a temperature of incubation of 28 degrees C, an incubation period of 7 days, and MIC-0.
Fernández-Torres
Belkys
B
Unitat de Microbiología, Facultat de Medicina, Universitat Rovira i Virgili, Reus, Barcelona, Spain.
Cabañes
Francisco J
FJ
Carrillo-Muñoz
Alfonso J
AJ
Esteban
Alexandre
A
Inza
Isabel
I
Abarca
Lourdes
L
Guarro
Josep
J
D000935:Antifungal Agents / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000187:drug effects* / Q000254:growth & development; D003022:Clotrimazole / Q000494:pharmacology*; D003470:Culture Media; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards; D009281:Naphthalenes / Q000494:pharmacology*; D015203:Reproducibility of Results; D013696:Temperature; D000077291:Terbinafine
D000935:Antifungal Agents; D003470:Culture Media; D009281:Naphthalenes; D017964:Itraconazole; D003022:Clotrimazole; D000077291:Terbinafine