title: Maturational and developmental competence of cumulus-free immature human oocytes derived from stimulated and intracytoplasmic sperm injection cycles.
abstract: The present experiments compared the maturational and developmental competence of immature oocytes derived from stimulated cycles, following culture in a newly designed in-vitro maturation medium (IVM-medium) or in standard tissue culture medium (TCM-199; control). The results indicated that maturation and fertilization rates were comparable when the cumulus-free M-I stage oocytes were matured in the IVM-medium (78.6%) or the control medium (70.8%). However, there was a significant difference in blastocyst development (P < 0.05) when M-I oocytes were matured in these two media (19.6 versus 7.7%). Both maturation and early embryonic development rates of GV-stage oocytes were significantly higher (P < 0.01) in the IVM-medium (maturation: 75.7%; blastocyst: 12.9%) compared with control (maturation 55.7%; blastocyst: 0.0%). Moreover, embryos developed to the blastocyst stage at a higher rate in both media if GV-stage oocytes had matured within 24 h compared with 48 h of culture. These results demonstrate that immature human oocytes derived from stimulated ovaries can achieve maturation and early embryonic development in vitro, especially in the new IVM-medium, which may allow additional embryos to be produced for clinical use at embryo transfer.
authors.affiliation: Department of Obstetrics and Gynecology, McGill University, Montreal, Montreal, Quebec, Canada. ri-cheng.chian@muhc.mcgill.ca
authors.forename: R C
authors.initials: RC
authors.lastname: Chian
authors.forename: S L
authors.initials: SL
authors.lastname: Tan
mesh_terms: D001755:Blastocyst / Q000166:cytology*; D002453:Cell Cycle; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D006801:Humans; D008297:Male; D008677:Metaphase; D009865:Oocytes / Q000166:cytology* / Q000502:physiology; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*
chemical_list: D003470:Culture Media