[Mitochondrial H+-ATPase: identification of subunits of the F0 subcomplex contacting with membrane lipids].
The subunits of the F0 membrane sector of bovine heart mitochondrial H(+)-ATPase that contact the lipids of the mitochondrial inner membrane were identified with the use of specially synthesized proteoliposomes that contained active mitochondrial H(+)-ATPase and a photoreactive lipid, which was 1-acyl-2-[12-(diazocyclopentadiene-2-carbonylamino)-[12-14C]dodecanoyl]-sn- glycero-3-phosphocholine, 1-acyl-2-[11-([125I]diazoiodocyclopentadiene-2-carbonyloxy)undecanoyl]-sn- glycero-3-phosphocholine, or 1-acyl-2-[12-(diazocyclopentadiene-2-carbonylamino)dodecanoyl]-sn-glycero- 3-phosphocholine, where acyl is a mixture of the residues of palmitic (70%) and stearic (30%) acids. An analysis of the cross-linked products obtained upon the UV-irradiation of these proteoliposomes indicated that subunits c and a of the F0 membrane sector contact the lipids. The cross-linked products were identified by SDS-PAGE and MALDI mass spectrometry.
Zaĭtseva
L G
LG
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia.
Ovchinnikova
T V
TV
Vodovozova
E L
EL
Molotkovskĭ
Iu G
IuG
Poliakov
N B
NB
Titov
M I
MI
Esipov
S E
SE
Grinkevich
V A
VA
D000818:Animals; D001345:Autoradiography; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D007425:Intracellular Membranes / Q000201:enzymology*; D008563:Membrane Lipids / Q000737:chemistry*; D008929:Mitochondria, Heart / Q000201:enzymology*; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry* / Q000302:isolation & purification; D021122:Protein Subunits / Q000737:chemistry* / Q000302:isolation & purification; D011510:Proteolipids / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D008563:Membrane Lipids; D021122:Protein Subunits; D011510:Proteolipids; C020485:proteoliposomes; D025261:Mitochondrial Proton-Translocating ATPases