title: Assembly of centrosomal proteins and microtubule organization depends on PCM-1.
abstract: The protein PCM-1 localizes to cytoplasmic granules known as "centriolar satellites" that are partly enriched around the centrosome. We inhibited PCM-1 function using a variety of approaches: microinjection of antibodies into cultured cells, overexpression of a PCM-1 deletion mutant, and specific depletion of PCM-1 by siRNA. All approaches led to reduced targeting of centrin, pericentrin, and ninein to the centrosome. Similar effects were seen upon inhibition of dynactin by dynamitin, and after prolonged treatment of cells with the microtubule inhibitor nocodazole. Inhibition or depletion of PCM-1 function further disrupted the radial organization of microtubules without affecting microtubule nucleation. Loss of microtubule organization was also observed after centrin or ninein depletion. Our data suggest that PCM-1-containing centriolar satellites are involved in the microtubule- and dynactin-dependent recruitment of proteins to the centrosome, of which centrin and ninein are required for interphase microtubule organization.
authors.affiliation: Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland, UK.
authors.forename: Alexander
authors.initials: A
authors.lastname: Dammermann
authors.forename: Andreas
authors.initials: A
authors.lastname: Merdes
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D000941:Antigens / Q000378:metabolism; D001324:Autoantigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016466:CHO Cells; D019556:COS Cells; D018797:Cell Cycle Proteins; D002502:Centrioles / Q000378:metabolism*; D002645:Chickens; D006224:Cricetinae; D003598:Cytoskeletal Proteins; D000072159:Dynactin Complex; D019204:GTP-Binding Proteins / Q000378:metabolism; D017353:Gene Deletion; D015870:Gene Expression / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008845:Microinjections; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D032446:Myoblasts / Q000166:cytology; D009687:Nuclear Proteins; D012516:Osteosarcoma; D034622:RNA Interference / Q000502:physiology; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000378:metabolism; D014981:Xenopus; D029867:Xenopus Proteins
chemical_list: D000906:Antibodies; D000941:Antigens; D001324:Autoantigens; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; C000606361:DCTN2 protein, human; C000606365:Dctn2 protein, mouse; D000072159:Dynactin Complex; D008869:Microtubule-Associated Proteins; C512806:NIN protein, human; C099035:Nin protein, mouse; D009687:Nuclear Proteins; C490113:PCM1 protein, Xenopus; C086074:PCM1 protein, human; C490114:Pcm1 protein, mouse; D029867:Xenopus Proteins; C086178:pericentrin; D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D019204:GTP-Binding Proteins