title: Regulative effect of IFN-gamma on the Fas/Fas L system of cholangiocarcinoma cells.
abstract: OBJECTIVE
  To detect the regulative effects of IFN-gamma on the expression of Fas and FasL of cholangiocarcinoma cells.
  
  
  METHODS
  We studied that the expression of Fas and FasL gene by the human cholangiocarcinoma cell line QBC939 by RT-PCR, Western blot, immunohistochemistry. At the same time, we investigated the regulative effect of IFN-gamma on them.
  
  
  RESULTS
  Fas and FasL mRNA and protein were expressed by cholangiocarcinoma cells. We also found IFN-gamma could upregulate the expression of the two genes (P < 0.01). However, IFN-gamma could also downregulate the ability of them to make Jurkat cells apoptotic. With the increasing of dosage and time, the effect was enhanced.
  
  
  CONCLUSIONS
  IFN-gamma could regulate the expression of Fas and FasL by cholangiocarcinoma cells, therefore it could reduce the ability of cholangiocarcinoma to occur immune escape. This provides new theoretical basis for immunological therapy of cholangiocarcinoma.
authors.affiliation: Department of General Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Ziyu
authors.initials: Z
authors.lastname: Li
authors.forename: Jianming
authors.initials: J
authors.lastname: Wang
authors.forename: Cong
authors.initials: C
authors.lastname: Tang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Shengquan
authors.initials: S
authors.lastname: Zou
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001650:Bile Duct Neoplasms; D001653:Bile Ducts, Intrahepatic; D018281:Cholangiocarcinoma; D006801:Humans; D008562:Membrane Glycoproteins; D012333:RNA, Messenger / Q000235:genetics; D019014:fas Receptor
chemical_list: D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D019014:fas Receptor