Effects of aging and caloric restriction on the gene expression of Foxo1, 3, and 4 (FKHR, FKHRL1, and AFX) in the rat skeletal muscles.
In C. elegans, insulin-like hormone signal pathway plays a significant role in longevity. In particular, daf-16 gene product is indispensable factor for this lifespan-extension. This signal pathway is critical for dauer formation, which is a similar state to hibernation in mammals. We examined the expression level of mammalian daf-16 homologues, Foxo 1,3, and 4 (FKHR, FKHRL1, and AFX) mRNAs in the rat skeletal muscles during aging and in 30% caloric restricted of ad libitum fed. The expression level of AFX mRNA was significantly higher at 6 and 12 months than at 3 and 26 months, and FKHRL1 expression was significantly higher at 6 months than at 3 and 26 months but FKHR expression showed no significant change with age. We observed a characteristic expression of AFX and FKHR mRNAs to be significantly higher in the second day in caloric restriction by every-other-day feeding than in ad libitum fed. This suggests that caloric restriction may increase the expression of FKHR-family genes and prevent the aging process in the skeletal muscles.
Furuyama
Tatsuo
T
Department of Molecular Genetics, National Institute for Longevity Sciences (NILS), Aichi, Japan. tfuruyam@nils.go.jp
Yamashita
Hitoshi
H
Kitayama
Kazuko
K
Higami
Yoshikazu
Y
Shimokawa
Isao
I
Mori
Nozomu
N
D000375:Aging / Q000378:metabolism*; D000818:Animals; D031204:Caloric Restriction; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins / Q000235:genetics*; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; D015870:Gene Expression; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D051381:Rats; D011916:Rats, Inbred F344; D014157:Transcription Factors / Q000235:genetics*; D000071161:Forkhead Box Protein O1
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; D009419:Nerve Tissue Proteins; D014157:Transcription Factors; C499515:FOXO3 protein, rat; C108803:FOXO4 protein, human; C074639:Foxo1 protein, rat; D000071161:Forkhead Box Protein O1