cDNA cloning and mRNA expression of Transformer 2 (Tra 2) in chicken embryo.
We report the cloning of a chicken Transformer 2 (Tra 2) cDNA that encodes a protein of 289 amino acids which are 97.9% identical to those of mammalian splicing factor, Tra 2. Tra 2 mRNA was expressed in chicken embryonic tissues and was observed as a band of 1.5 kb by Northern blot analysis. Whole mount in situ hybridization showed an mRNA expression of Tra 2 in telencephalon, mandible, hyoid arch, wing and leg buds as early as day 3.5 of incubation. These results suggest that the Tra 2 gene may play a role in organogenesis in the chicken embryo.
Yamamoto
Ichiro
I
Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan.
Tsukada
Akira
A
Saito
Noboru
N
Shimada
Kiyoshi
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002642:Chick Embryo; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D029721:Drosophila Proteins; D017403:In Situ Hybridization; D018878:Limb Buds; D008334:Mandible / Q000196:embryology / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D012261:Ribonucleoproteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D013687:Telencephalon / Q000196:embryology / Q000378:metabolism; D014921:Wings, Animal / Q000196:embryology / Q000378:metabolism
D018076:DNA, Complementary; D029721:Drosophila Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C066174:tra2 protein, Drosophila