Expression of the rice vdac isoform2: histochemical localization and expression level.
The voltage-dependent anion-selective channel (VDAC) is a mitochondrial outer membrane ion channel. The putative promoter of the rice vdac isoform2 (osvdac2) was isolated by screening a rice genomic library. Computer-based analyses predicted a TATA box, a putative transcription start and several transcription factor-binding sites including pollen specific elements. The promoter region was fused to the gus reporter gene and introduced into rice by Agrobacterium-mediated transformation. Histochemical and cell-type localizations indicated an overall expression of this promoter with a strong expression in actively growing lateral roots and in the pollen grains. Quantitative experiments showed that the osvdac2 promoter has a strong specific activity in both root and shoot. Thus, the osvdac2 promoter could be a good alternative to viral promoters (e.g. CaMV 35S) to overexpress genes in transgenic Poaceae.
Al Bitar
Fawaz
F
Laboratoire de Physiologie Végétale, Université Libre de Bruxelles, Campus Plaine (CP 206/2), B-1050, Brussels, Belgium.
Roosens
Nancy
N
Boxtel
Jos Van
JV
Dewaele
Eric
E
Jacobs
Michel
M
Homblé
Fabrice
F
D001483:Base Sequence; D020869:Gene Expression Profiling; D005822:Genetic Vectors; D015698:Genomic Library; D024101:Mitochondrial Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D018517:Plant Roots / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism; D011058:Pollen / Q000378:metabolism; D018272:Porins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic; D014162:Transfection; D019076:Transgenes; D050994:Voltage-Dependent Anion Channels
D024101:Mitochondrial Proteins; D010940:Plant Proteins; D018272:Porins; D050994:Voltage-Dependent Anion Channels