title: Transcription initiation sites and promoter structure of the human TRAIL-R3 gene.
abstract: TRAIL-R3 is a decoy receptor for TRAIL (tumor necrosis factor-related apoptosis-inducing ligand), a member of the tumor necrosis factor ligand family. In several cell types decoy receptors inhibit TRAIL-induced apoptosis by binding TRAIL and preventing its binding to TRAIL pro-apoptotic receptors. Here we report the cloning of the promoter region of human TRAIL-R3 and the mapping of the transcriptional start sites. This gene contains a consensus TATA box and the minimal promoter lies within the first 33 nucleotides upstream of the transcription start site. Transient transfection assays of luciferase reporter plasmids demonstrate that human TRAIL-R3 promoter can be induced in doxorubicin-treated MCF-7 cells in a p53-independent manner.
authors.affiliation: Instituto de Parasitología y Biomedicina, CSIC, calle Ventanilla 11, Granada, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Ruiz de Almodóvar
authors.forename: Abelardo
authors.initials: A
authors.lastname: López-Rivas
authors.forename: Juan Miguel
authors.initials: JM
authors.lastname: Redondo
authors.forename: Antonio
authors.initials: A
authors.lastname: Rodríguez
mesh_terms: D024506:5' Flanking Region; D000970:Antineoplastic Agents / Q000494:pharmacology; D001483:Base Sequence; D003001:Cloning, Molecular; D004317:Doxorubicin / Q000494:pharmacology; D058851:GPI-Linked Proteins; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis / Q000235:genetics*; D053320:Receptors, Tumor Necrosis Factor, Member 10c; D024363:Transcription Initiation Site; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured; D053319:Tumor Necrosis Factor Decoy Receptors; D016159:Tumor Suppressor Protein p53 / Q000502:physiology
chemical_list: D000970:Antineoplastic Agents; D058851:GPI-Linked Proteins; D012333:RNA, Messenger; D018124:Receptors, Tumor Necrosis Factor; D053320:Receptors, Tumor Necrosis Factor, Member 10c; C108661:TNFRSF10C protein, human; D053319:Tumor Necrosis Factor Decoy Receptors; D016159:Tumor Suppressor Protein p53; D004317:Doxorubicin