title: The phthalate diesters DEHP and DBP do not induce lauric acid hydroxylase activity in rainbow trout.
abstract: Aquatic organisms are extensively exposed to phthalate esters. We have investigated in trout the effects of diethylhexylphthalate (DEHP) and dibutylphthalate (DBP) on xenobiotic metabolizing enzymes which have been suggested as possible environmental biomarkers. Rainbow trout (Oncorhynchus mykiss) were waterborne exposed to DEHP (1 mg/l) or DBP (0.1 or 1 mg/l) for 72 h. Another group of rainbow trout received daily for 3 days an intraperitoneal injection of 50 mg/kg of DEHP or DBP. Laurate hydroxylation, ethoxyresorufin-o-deethylation, UDP-glucuronyltransferase activity and glutathione-S-transferase activity were measured in liver and extrahepatic tissues. The phthalate esters have been found not to induce these enzymes; in particular, the results do not support the previously described induction of lauric acid hydroxylase in sea bass treated with DEHP [Comp. Biochem. Physiol. B122 (1999) 253.].
authors.affiliation: UMR 1089 Xénobiotiques, INRA/ENSAT/ENVT, Toulouse, France. jcravedi@toulose.inra.fr
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Cravedi
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Perdu-Durand
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D042926:Cytochrome P-450 CYP4A; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D003993:Dibutyl Phthalate / Q000494:pharmacology*; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D004781:Environmental Exposure; D004790:Enzyme Induction; D008099:Liver / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D017686:Oncorhynchus mykiss / Q000502:physiology*; D014874:Water Pollutants, Chemical / Q000494:pharmacology*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D003993:Dibutyl Phthalate; D003577:Cytochrome P-450 Enzyme System; D004051:Diethylhexyl Phthalate; D006899:Mixed Function Oxygenases; D042926:Cytochrome P-450 CYP4A