Expression of CD64 as a potential marker of neonatal sepsis.
The aim of this study was to identify a novel immunological indicator useful for the early diagnosis (through a rapid and single determination) of neonatal sepsis (NS). Peripheral blood samples were taken from 63 neonates, who were classified into four groups: proven NS (n = 17); clinical NS (n = 14); disease without infection (n = 17); and healthy newborns (n = 15). Neutrophil expression of CD64, CD43, CD44, CD50, CD62L and Mac-1, and plasma levels of interleukin (IL)-1beta, IL-6, tumor necrosis factor-alpha (TNF-alpha) and soluble L-selectin (sCD62L), were determined. Expression of CD64 was significantly enhanced in the group with proven sepsis and clinical NS compared to newborns without infection (p < 0.05). Eight newborns with proven or clinical sepsis, but only one with disease without infection, showed an increased percentage of CD64+ cells (diagnostic specificity = 96.8%). No significant differences were found in the expression of the other leucocyte differentiation antigens studied. As previously described, TNF-alpha and IL-6 levels were significantly elevated in newborns with proven or clinical sepsis compared to neonates without infection (p < 0.05). Our results suggest that, through a single determination, the enhanced expression of CD64 is a highly specific indicator of NS, although its diagnostic sensitivity is low (25.8%). In contrast, we found that plasma levels of IL-1beta and sCD62L, as well as the expression of Mac-1, CD43, CD44, CD50, and CD62L, do not appear to be useful for the diagnosis of NS.
Layseca-Espinosa
Esther
E
Department of Immunology, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, México.
Pérez-González
Luis F
LF
Torres-Montes
Abraham
A
Baranda
Lourdes
L
de la Fuente
Hortensia
H
Rosenstein
Yvonne
Y
González-Amaro
Roberto
R
D015703:Antigens, CD / Q000096:biosynthesis / Q000097:blood; D015415:Biomarkers / Q000097:blood; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood; D006801:Humans; D007229:Infant Welfare; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000175:diagnosis* / Q000378:metabolism; D007710:Klebsiella Infections / Q000175:diagnosis / Q000378:metabolism; D007711:Klebsiella pneumoniae; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000096:biosynthesis; D008800:Mexico; D009504:Neutrophils / Q000378:metabolism; D019038:Platelet Glycoprotein GPIb-IX Complex; D011237:Predictive Value of Tests; D017452:Receptors, IgG / Q000096:biosynthesis* / Q000097:blood; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000175:diagnosis* / Q000378:metabolism
D015703:Antigens, CD; D015415:Biomarkers; D016207:Cytokines; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D019038:Platelet Glycoprotein GPIb-IX Complex; D017452:Receptors, IgG; C094990:adhesion receptor