title: Determination of undecylenic and sorbic acids in cosmetic preparations by high performance liquid chromatography with electrochemical detection.
abstract: A highly sensitive and selective method for the determination of sorbic (SA) and undecylenic acid (UA) in cosmetic formulations by a high performance liquid chromatography method with electrochemical detection (ECD) is described. The pre-column derivatizations of SA and UA and the internal standard (cyclohexanoic acid (cHA)) were carried out using 1-(2,5-dihydroxyphenyl)-2-bromoethanone (2,5-DBE) as an electroactive labeling reagent previously synthesized in our lab. The resulting electroactive esters were separated by isocratic elution of a 5 micrometer Hypersil CN column with acetonitrile-acetate buffer eluent. The compounds were detected by a porous graphite electrode set at an oxidation potential of +0.45 V. The analytical method developed in this study is suitable for quality control assays of complex cosmetic formulations containing sorbic and/or UA.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università degli Studi di Catania, Viale Andrea Doria 6, 95125, Catania, Italy. bousquet@unict.it
authors.forename: Ennio
authors.initials: E
authors.lastname: Bousquet
authors.forename: A
authors.initials: A
authors.lastname: Spadaro
authors.forename: N A
authors.initials: NA
authors.lastname: Santagati
authors.forename: S
authors.initials: S
authors.lastname: Scalia
authors.forename: G
authors.initials: G
authors.lastname: Ronsisvalle
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003358:Cosmetics / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D013011:Sorbic Acid / Q000032:analysis* / Q000737:chemistry; D014476:Undecylenic Acids / Q000032:analysis* / Q000737:chemistry
chemical_list: D003358:Cosmetics; D014476:Undecylenic Acids; D013011:Sorbic Acid