Quantitative analysis of selegiline and three metabolites (N-desmethylselegiline, methamphetamine, and amphetamine) in human plasma by high-performance liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry.
This report describes a sensitive and specific high-performance liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry method for the detection of subnanogram concentrations of selegiline and its three principle metabolites, N-desmethylselegiline, methamphetamine, and amphetamine, in human plasma. The assay has a dynamic range of 0.1-20 ng/mL for selegiline and N-desmethylselegiline (norselegiline) and 0.2-20 ng/mL for methamphetamine and amphetamine. The inter- and intra-assay precision and accuracy varied by less than 11% for all analytes at 0.3, 2.5, and 15 ng/mL and less than 16% at the lower limit of quantitation (0.1 ng/mL for selegiline and norselegiline; and 0.2 ng/mL for methamphetamine and amphetamine). Selegiline and its metabolites showed no significant loss in quantitative accuracy after three freeze/thaw cycles or after up to 6 h at room temperature prior to extraction. Extracted plasma samples retained quantitative accuracy after storage for at least 7 days at -20 degrees C or up to 70 h at room temperature. Methanolic stock solutions were stable for at least 6 h when kept at room temperature or at least 90 days when kept at -20 degrees C.
Slawson
Matthew H
MH
Department of Pharmacology and Toxicology, University of Utah Health Sciences Center, Salt Lake City 84112, USA. matt.slawson@m.cc.utah.edu
Taccogno
James L
JL
Foltz
Rodger L
RL
Moody
David E
DE
D000661:Amphetamine / Q000032:analysis; D000662:Amphetamines / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005615:Freezing; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008694:Methamphetamine / Q000032:analysis; D008996:Monoamine Oxidase Inhibitors / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012642:Selegiline / Q000031:analogs & derivatives / Q000097:blood* / Q000493:pharmacokinetics
D000662:Amphetamines; D008996:Monoamine Oxidase Inhibitors; D012642:Selegiline; D008694:Methamphetamine; C017861:desmethylselegiline; D000661:Amphetamine