title: AcePrimer: automation of PCR primer design based on gene structure.
abstract: AcePrimer is an internet-accessed application based on CGI/Perl programming that designs PCR primers to search for deletion alleles in Caenorhabditis elegans gene knockout experiments and uses electronic PCR to search the entire genomic DNA sequence for potential false priming or multiple PCR amplification targets. Features such as the ability to target specific exons with the 'poison primer' approach and evaluation of primers with electronic PCR provide a flexible, web-based approach to design effective primers whilst minimizing the need for empirical optimization of PCR experiments.
authors.affiliation: Genome Sciences Centre, British Columbia Cancer Agency, 600 W 10th Avenue, Vancouver, BC, Canada V5Z 4E6.
authors.forename: Sheldon J
authors.initials: SJ
authors.lastname: McKay
authors.forename: Steven J M
authors.initials: SJ
authors.lastname: Jones
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics*; D003198:Computer Simulation; D017931:DNA Primers / Q000235:genetics*; D003628:Database Management Systems; D030561:Databases, Nucleic Acid; D017353:Gene Deletion; D020407:Internet; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software; D014584:User-Computer Interface
chemical_list: D017931:DNA Primers