title: gyrA gene mutations in quinolone-resistant Shigellae flexneri.
abstract: OBJECTIVE: To study the resistance mechanism of quinolone against Shigellae flexneri. METHODS: The N-terminal coding region of gyrA gene of 38 quinolone-resistant Shigellae flexneri were amplified by PCR and mutations detected by single- strand conformation polymorphism (SSCP), followed by sequence analysis. RESULTS: Eight isolates were found to be positive for gyrA mutations, and DNA sequence analysis of gyrA gene revealed 2 mutations that resulted in changes of the amino acids (Ser-83--Leu, Asp-87--Gly). CONCLUSION: gryA gene mutations is closely related to high resistance of Shigellae flexneri to quinolone.
authors.affiliation: Department of Medical Teaching and Research, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Xiang
authors.forename: Shou-Yi
authors.initials: SY
authors.lastname: Yu
authors.forename: Hong
authors.initials: H
authors.lastname: Wang