Abnormal protein patterns in blood serum and cerebrospinal fluid detected by capillary electrophoresis.
Capillary zone electrophoresis and high-resolution agarose gel electrophoresis were compared to detect protein components in serum and cerebrospinal fluid of patients. Both electrophoretic methods proved to be useful for detection of protein abnormalities (e.g., mono- and oligoclonal bands) in biological fluids, but capillary electrophoresis offered several important advantages, such as sample application without preliminary concentration, lack of staining procedures, and on-line evaluation of patterns. Furthermore, capillary electrophoresis exhibits shorter analysis time and high resolution with low baseline noise. The results were proven to be powerful in diagnosis and monitoring of dyscrasias in routine laboratory practice.
Ivanova
Mariella
M
Department of Neurology, Biochemistry Laboratory, Medical University, 1 Georgy Sofijski Str., 1431 Sofia, Bulgaria.
Tzvetanova
Elena
E
Jetcheva
Veska
V
Kilár
Ferenc
F
D000293:Adolescent; D000328:Adult; D000368:Aged; D001797:Blood Protein Electrophoresis / Q000379:methods; D001798:Blood Proteins / Q000032:analysis*; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006521:Hepatitis, Chronic / Q000097:blood / Q000134:cerebrospinal fluid; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000097:blood / Q000134:cerebrospinal fluid; D009422:Nervous System Diseases / Q000097:blood / Q000134:cerebrospinal fluid; D011506:Proteins
D001798:Blood Proteins; D002556:Cerebrospinal Fluid Proteins; D011506:Proteins