title: [Decreased antioxidant-defence mechanisms in cerebrospinal fluid (CSF) in patients with tick-borne encephalitis (TBE)].
abstract: 14 patients with Tick-borne Encephalitis (TBE) aged 21-64 (mean = 42.3) were analysed. The activity of superoxide dismutase (SOD), glutathione reductase (GSSG-R), glutathione peroxidase (GSH-Px), concentrations of malondialdehyde (MDA) and total sulphydryl groups (-SH) were measured in cerebrospinal fluid (CSF). Control group consisted of 10 patients whose CSF parameters remained in normal range. The CSF examination was performed twice: before and 3 weeks after treatment. The analysed activity of SOD, GSH-Px, GSSG-R, MDA and total sulphydryl groups (-SH) during the acute stage of the disease was significantly lower comparing to the control group. Despite the treatment, GSSG-R activity, MDA concentration and total sulphydryl groups--SH further lowered significantly. Although the SOD activity in CSF was higher in the second examination, it remained significantly lower comparing to the control group. We showed that the GSH-Px and GSSG-R activity in CSF after the acute stage of the TBE remained significantly lower than in the control group. Our examinations prove that during the TBE an increased generation of oxygen-derived free radicals occurs what shows decreased activity of the antioxidant parameters (SOD, GSH-Px, GSSG-R) and decreased concentration of total sulphydryl groups--SH in CSF. Our results suggest that during TBE, molecular structures injury of enzymes and antioxidative reactive cofactors may occur.
authors.affiliation: Kliniki Chorób Zakaźnych i Neuroinfekcji AM w Białymstoku.
authors.forename: Sławomir A
authors.initials: SA
authors.lastname: Pancewicz
authors.forename: Teresa
authors.initials: T
authors.lastname: Hermanowska-Szpakowicz
authors.forename: Marzena
authors.initials: M
authors.lastname: Makarewicz-Płońska
authors.forename: Artur
authors.initials: A
authors.lastname: Witek
authors.forename: Ryszard
authors.initials: R
authors.lastname: Farbiszewski
authors.forename: Joanna
authors.initials: J
authors.lastname: Zajkowska
authors.forename: Bozena
authors.initials: B
authors.lastname: Michalska
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000134:cerebrospinal fluid; D016022:Case-Control Studies; D004675:Encephalitis, Tick-Borne / Q000134:cerebrospinal fluid* / Q000201:enzymology; D005260:Female; D016166:Free Radical Scavengers / Q000134:cerebrospinal fluid*; D005979:Glutathione Peroxidase / Q000134:cerebrospinal fluid; D005980:Glutathione Reductase / Q000134:cerebrospinal fluid; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000134:cerebrospinal fluid; D008875:Middle Aged; D010088:Oxidoreductases / Q000134:cerebrospinal fluid*; D013482:Superoxide Dismutase / Q000134:cerebrospinal fluid; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D016166:Free Radical Scavengers; D008315:Malondialdehyde; D010088:Oxidoreductases; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase