Routes for salmonella contamination of poultry meat: epidemiological study from hatchery to slaughterhouse.
Data were collected on the prevalence of salmonella at different stages during the life cycle of 18 broiler flocks on different farms as well as during slaughter in different poultry slaughterhouses. For the isolation of salmonella, the highest sensitivity (93.9%) was obtained by enrichment in the semi-solid agar Diasalm. The 'overshoe method' utilizing several pairs of overshoes provided the highest sensitivity for determining the salmonella status of the broilers during rearing. A clear decrease of the relative importance of the first production stages was demonstrated for the salmonella contamination of the end product, whereas horizontal transmission of salmonella to broilers during rearing and to broiler carcasses in the slaughterhouse was shown to be the main determinative factor. Ten of the 18 flocks received a salmonella positive status with the highest shedding occurring during the first 2 weeks of rearing. The shedding of the animals was significantly negatively influenced by the use of subtherapeutic or therapeutic doses of antibiotics. The intake of portable material in the broiler house was identified as the most important risk factor for horizontal transmission. Significant associations were found between the contamination level of a flock and hygiene of the broiler house, feed and water in the broiler house and both animal and non-animal material sampled in the environment. No correlation was found between contamination during the rearing period and contamination found after slaughtering. The presence of faecal material in the transport crates and predominantly the identity of the slaughterhouse seemed to be the determining factors for carcass quality. Improved hygiene management during transport of broilers and in some slaughterhouses could significantly reduce the risk of salmonella contamination of poultry meat.
Heyndrickx
M
M
Center for Agricultural Research, Department for Animal Product Quality, Melle, Belgium.
Vandekerchove
D
D
Herman
L
L
Rollier
I
I
Grijspeerdt
K
K
De Zutter
L
L
D000822:Animal Husbandry; D000818:Animals; D001530:Belgium / Q000453:epidemiology; D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial / Q000302:isolation & purification*; D018562:Disease Transmission, Infectious; D005516:Food Microbiology / Q000592:standards*; D016133:Polymerase Chain Reaction; D011200:Poultry / Q000382:microbiology; D011201:Poultry Diseases / Q000453:epidemiology* / Q000382:microbiology / Q000517:prevention & control / Q000635:transmission*; D015995:Prevalence; D012307:Risk Factors; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification; D012481:Salmonella Infections, Animal / Q000453:epidemiology* / Q000382:microbiology / Q000517:prevention & control / Q000635:transmission*; D012680:Sensitivity and Specificity; D014186:Transportation
D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial