title: Lymphoid and myeloid neoplasms involving cerebrospinal fluid: comparison of morphologic examination and immunophenotyping by flow cytometry.
abstract: We studied 53 samples of cerebrospinal fluid (CSF) by cytologic examination and immunophenotyping by flow cytometry. The samples were taken from 43 patients; 25 had a previous diagnosis of malignant lymphoma/leukemia and the remaining 18 a variety of other diseases involving the central nervous system (CNS). Lymphoma/leukemia was detected in 21 samples: 12 by morphologic examination and immunophenotyping and nine by immunophenotyping alone. There were two cases with a suspicious morphologic examination and negative immunophenotyping in which the final diagnosis were cryptococcal and viral meningitis. In the group of 18 patients, one was diagnosed as a primary malignant lymphoma of the CNS and was positive with cytology and immunophenotyping. The other 17 were negative with both methods and follow-up showed no evidence of lymphoma/leukemia. This study shows that morphologic examination combined with flow cytometry enhances the detection rate by 75% over morphologic examination alone in CSF samples.
authors.affiliation: Center for Medical Education and Clinical Investigations, Buenos Aires, Argentina. andresroma@hotmail.com
authors.forename: Andres A
authors.initials: AA
authors.lastname: Roma
authors.forename: Alejandro
authors.initials: A
authors.lastname: Garcia
authors.forename: Alejandra
authors.initials: A
authors.lastname: Avagnina
authors.forename: Carla
authors.initials: C
authors.lastname: Rescia
authors.forename: Boris
authors.initials: B
authors.lastname: Elsner
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D002493:Central Nervous System Diseases / Q000134:cerebrospinal fluid / Q000473:pathology; D002555:Cerebrospinal Fluid / Q000166:cytology*; D002648:Child; D003581:Cytodiagnosis / Q000379:methods; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016130:Immunophenotyping; D007938:Leukemia / Q000134:cerebrospinal fluid* / Q000473:pathology; D008223:Lymphoma / Q000134:cerebrospinal fluid* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity
chemical_list: D015703:Antigens, CD