title: Biomarkers for endocrine disruptors in three species of Mediterranean large pelagic fish.
abstract: The hypothesis that Mediterranean top predator species, such as large pelagic fish, are potentially at risk due to endocrine disrupting chemicals (EDCs), is investigated. The potential estrogenic effects of PHAHs in three fish species of commercial interest, the top predators bluefin tuna (Thunnus thynnus thynnus), swordfish (Xiphias gladius), and Mediterranean spearfish (Tetrapturus belone), were investigated using vitellogenin (Vtg), zona radiata proteins (Zrp) and mixed function oxidases (EROD, BPMO) as diagnostic tools. High induction of Vtg and Zrp was detected by western blot and ELISA techniques in adult males of X. gladius and T. thynnus thynnus, suggesting that these species are at high toxicological risk in the Mediterranean sea. Comparison of BPMO and EROD activities in the three species indicated, both in male and female, much higher MFO activity in bluefin tuna. This data suggests high exposure of this species to lipophilic xenobiotic contaminants in the Mediterranean environment.
authors.affiliation: Department of Animal Biology and Marine Ecology, University of Messina, Italy. cdfos@tin.it
authors.forename: M C
authors.initials: MC
authors.lastname: Fossi
authors.forename: S
authors.initials: S
authors.lastname: Casini
authors.forename: L
authors.initials: L
authors.lastname: Marsili
authors.forename: G
authors.initials: G
authors.lastname: Neri
authors.forename: G
authors.initials: G
authors.lastname: Mori
authors.forename: S
authors.initials: S
authors.lastname: Ancora
authors.forename: A
authors.initials: A
authors.lastname: Moscatelli
authors.forename: A
authors.initials: A
authors.lastname: Ausili
authors.forename: G
authors.initials: G
authors.lastname: Notarbartolo-di-Sciara
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D004527:Egg Proteins / Q000032:analysis / Q000096:biosynthesis; D004703:Endocrine System / Q000187:drug effects*; D004781:Environmental Exposure; D005260:Female; D008297:Male; D006899:Mixed Function Oxygenases / Q000032:analysis / Q000494:pharmacology*; D010473:Perciformes / Q000502:physiology*; D014819:Vitellogenins / Q000032:analysis / Q000096:biosynthesis*; D014874:Water Pollutants, Chemical / Q000009:adverse effects; D015262:Xenobiotics / Q000009:adverse effects*
chemical_list: D015415:Biomarkers; D004527:Egg Proteins; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D015262:Xenobiotics; C423083:zona radiata protein, fish; D006899:Mixed Function Oxygenases