Mannheimia haemolytica serotype 1 and Pasteurella trehalosi serotype 10 culture supernatants contain fibrinogen-binding proteins.
Fibrinogen-binding proteins were found in the culture supernatants of Mannheimia haemolytica serotype 1 (ATCC 43270) and Pasteurella trehalosi serotype 10 (ECO-100). Sheep fibrinogen was biotinylated and shown to bind to proteins in the culture supernatants by modified western blot. Fibrinogen-binding proteins in the culture supernatant may be important virulence factors leading to the characteristic fibrinous pneumonia caused by these organisms and may be critical antigenic targets for immune prophylaxis.
McNeil
Heather J
HJ
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Ont, N1G 2W1, Guelph, Canada. hmcneil@uoguelph.ca
Shewen
Patricia E
PE
Lo
Reggie Y C
RY
Conlon
Jennifer A
JA
Miller
Michael W
MW
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D015153:Blotting, Western; D017077:Culture Media, Conditioned / Q000737:chemistry*; D005340:Fibrinogen / Q000378:metabolism*; D016978:Mannheimia haemolytica / Q000737:chemistry / Q000378:metabolism* / Q000472:pathogenicity; D010325:Pasteurella / Q000737:chemistry / Q000378:metabolism* / Q000472:pathogenicity; D011485:Protein Binding; D012756:Sheep
D001426:Bacterial Proteins; D017077:Culture Media, Conditioned; D005340:Fibrinogen