Expression profiling of periodontal ligament cells stimulated with enamel matrix proteins in vitro: a model for tissue regeneration.
Several studies have examined the role of enamel matrix proteins in root formation and periodontal regeneration, although most of these have focused on a few specific genes which had previously been implicated. However, recent advances in expressional profiling have made it possible to examine the range of genetic responses involved in these processes. In the present experiments, we have therefore utilized this technique to determine the effects of enamel matrix proteins on the gene activities of periodontal ligament cells in vitro. Such cells were found to have an elevated level of RNA synthesis compared with control cells. Moreover, hybridization of the cDNA prepared from this RNA to gene array filters showed that there was differential expression of 121 genes, most of which had not previously been associated with periodontal regeneration. Some of these selective changes in gene activity might thus reflect the fundamental events that underlie periodontal development.
Brett
P M
PM
Department of Periodontology, Eastman Dental Institute, University College London, 256 Gray's Inn Road, London WC1X 8LD, UK.
Parkar
M
M
Olsen
I
I
Tonetti
M
M
D000328:Adult; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D012038:Regeneration / Q000235:genetics*
D003746:Dental Enamel Proteins; C109069:enamel matrix proteins; D012313:RNA; D004247:DNA