[Pathological research in experimental diabetes in rat].
OBJECTIVE
To study dynamically the pathological changes of diabetic retinopathy (DR).


METHODS
The techniques of light microscopy, immunohistochemical staining, electron microscopy and histochemical electron microscopy were used to quantitatively analyze the Type IV collagen, laminin and their anionic sites in the rat retinal capillary basement membrane (RBM).


RESULTS
RBM became gradually thickened and was accompanied with an increased staining reaction for type IV collagen and laminin and decrease of anionic sites in number.


CONCLUSIONS
The increase in thickness, in type IV collagen and laminin and the decrease of the anionic sites in the RBM may be the pathological changes of the exudative lesion in DR.
Wei
Lingge
L
Department of Ophthalmology, The second Affiliated Hospital, Hebei Medical University, Shijiazhuang 050000, China. wlg522@hotmail.com
Wang
Changling
C
Duan
Huijun
H
D000818:Animals; D001485:Basement Membrane / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D002196:Capillaries / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure; D024141:Collagen Type IV / Q000378:metabolism; D003930:Diabetic Retinopathy / Q000378:metabolism / Q000473:pathology*; D005260:Female; D007150:Immunohistochemistry; D007797:Laminin / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure; D013997:Time Factors
D024141:Collagen Type IV; D007797:Laminin