Dietary mannan-oligosaccharides and their effect on chicken caecal microflora in relation to Salmonella Enteritidis colonization.
This study first investigates the effects of mash diet, or mash supplemented with either 2.5% mannose-oligosaccharide (MOS) or palm kernel meal (PKM), on the microflora of the hen caecal contents. Second, it investigates the effect of caecal contents of hens (HCC) fed mash or mash supplemented with MOS or PKM on the major microflora groups of chicks, and their inhibitory effect on Salmonella enterica serovar Enteritidis (PT4) colonization. Finally, this study investigates the effect over time of diets supplemented with MOS or PKM on S. Enteritidis colonization and the microflora of chicks. In hens, supplemented diets increased Bifidobacterium spp., while decreasing members of Enterobacteriaceae and Enterococcus spp., compared with the mash diet. Chicks dosed with the HCC showed, on average, increased numbers of anaerobes, while the numbers of aerobes decreased including coliforms and S. Enteritidis compared with controls without HCC. In chicks fed the MOS-supplemented or PKM-supplemented diets, S. Enteritidis colonization decreased over time, compared with mash alone. Four-week-old PKM birds showed an increase in Bifidobacterium spp. and Lactobacillus spp., with a decrease in S. Enteritidis compared with week 2. Generally, the HCC and diets supplemented with MOS or PKM affected the birds intestinal microflora by increasing the Bifidobacterium spp. and Lactobacillus spp., while decreasing the Enterobacteriaceae groups. They also reduced susceptibility in young chickens to colonization by S. Enteritidis.
Fernandez
F
F
Division of Food Animal Science, Department of Clinical Veterinary Science, University of Bristol, Langford, Avon BS40 5DU, UK. Fresie.Fernandez@bristol.ac.uk
Hinton
M
M
Van Gils
B
B
D000821:Animal Feed; D000818:Animals; D002432:Cecum / Q000187:drug effects* / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology*; D004032:Diet; D019587:Dietary Supplements; D005260:Female; D005766:Gastrointestinal Contents / Q000187:drug effects / Q000382:microbiology; D008297:Male; D008351:Mannans / Q000008:administration & dosage / Q000494:pharmacology*; D009844:Oligosaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000517:prevention & control; D012477:Salmonella enteritidis / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors
D008351:Mannans; D009844:Oligosaccharides