Vancomycin encapsulation in biodegradable poly(epsilon-caprolactone) microparticles for bone implantation. Influence of the formulation process on size, drug loading, in vitro release and cytocompatibility.
Vancomycin encapsulation in biodegradable poly(epsilon-caprolactone) microparticles (200 microm mean diameter) was most efficient with a simple emulsion technique that dispersed 122.5 mg/g of polymer. Scanning electron micrographs showed smooth or pitted particles. Dissolution studies were correlated with microparticle morphology, indicating higher release with pitted particles when vancomycin was encapsulated in a dissolved state. The cytocompatibility of these poly(epsilon-caprolactone) microparticles was demonstrated by a direct contact cytotoxic assay. This material can be considered as an efficient drug delivery system for bone implantation.
Le Ray
A-M
AM
Laboratoire de Pharmacie Galénique, Centre de Recherche sur les Matériaux d'intérêt Biologique, Equipe INSERM 99-03, BP84215, 44042 Nantes cedex, France.
Chiffoleau
S
S
Iooss
P
P
Grimandi
G
G
Gouyette
A
A
Daculsi
G
G
Merle
C
C
D020341:Absorbable Implants; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology; D001672:Biocompatible Materials / Q000494:pharmacology*; D018786:Bone Substitutes; D002208:Caproates / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line; D016503:Drug Delivery Systems; D005347:Fibroblasts / Q000378:metabolism; D007700:Kinetics; D007783:Lactones / Q000737:chemistry* / Q000494:pharmacology*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D013053:Spectrophotometry; D013997:Time Factors; D014640:Vancomycin / Q000008:administration & dosage / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D018786:Bone Substitutes; D002208:Caproates; D007783:Lactones; C121056:caprolactone; D014640:Vancomycin