Inhibition of protein synthesis by aminoglycoside-arginine conjugates.
Inhibition of translation by small molecule ligands has proven to be a useful tool for understanding this complex cellular mechanism, as well as providing drugs of significant medical importance. Many small molecule ligands inhibit translation by binding to RNA or RNA/protein components of the ribosomal subunits and usurping their function. A class of peptidomimetics [aminoglycoside-arginine conjugates (AAC)] has recently been designed to inhibit HIV TAR/tat interaction and in experiments aimed at assessing the inhibitory effects of AACs on TAR-containing transcripts, we found that AACs are general inhibitors of translation. Experiments reported herein aim at characterizing these novel properties of AACs. We find that AACs are inhibitors of eukaryotic and prokaryotic translation and exert their effects by blocking peptide chain elongation. Structure/activity relationship studies suggest that inhibition of translation by AACs is directly related to the number of arginine groups present on the aminoglycoside backbone and to the nature of the core aminoglycoside. AACs are therefore attractive tools for understanding and probing ribosome function.
Carriere
Marjolaine
M
Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
Vijayabaskar
Veerappan
V
Applefield
Drew
D
Harvey
Isabelle
I
Garneau
Philippe
P
Lorsch
Jon
J
Lapidot
Aviva
A
Pelletier
Jerry
J
D000617:Aminoglycosides / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001120:Arginine / Q000737:chemistry / Q000494:pharmacology; D005057:Eukaryotic Cells / Q000502:physiology; D005260:Female; D005601:Framycetin / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D016325:HIV Long Terminal Repeat / Q000235:genetics; D020128:Inhibitory Concentration 50; D009865:Oocytes / Q000502:physiology; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D011500:Protein Synthesis Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014982:Xenopus laevis
D000617:Aminoglycosides; D010455:Peptides; D011500:Protein Synthesis Inhibitors; C444521:neomycin B-arginine conjugate NeoR; D005601:Framycetin; D001120:Arginine