title: In vitro effects of ascorbate and Trolox on the biocompatibility of dental restorative materials.
abstract: Previous in vitro studies on the cytotoxicity of eight dental restorative materials including composites, compomers, resin-modified glass ionomer cements and glass ionomer cements have demonstrated a depletion of intracellular glutathione in gingival fibroblasts incubated with eluates of these materials and a protective effect of N-acetylcysteine. In the present study, we investigate the effects of two other antioxidants: ascorbate and Trolox. It was found that Trolox reduced the cytotoxicity induced by resin-based biomaterial eluates. In contrast, ascorbate increased in a dose-dependent manner the toxic effect of all eluates except for Z100 MP and Tetric flow (composites). The effect of D-mannitol was studied for GC FUJI II and was found to neutralize the additional toxic effect of ascorbate. Ascorbate increased the depletion of intracellular glutathione of these dental material eluates (between 17% and 24%, depending on the material). Quantification of metal ions in the dental material eluates showed the presence of significant amounts of aluminum and iron in GC FUJI II > photac fil > GC FUJI II LC > F2000. The mechanism of this increased cytotoxicity could be explained by the Fenton reaction resulting from the pro-oxidant effect of ascorbate in the presence of iron (transition metal ions) and/or aluminum.
authors.affiliation: Laboratoire de Biologie et Physiopathologie Cranio-faciales, EA 2496, Faculté de Chirurgie Dentaire, Université Paris V, 1 rue Maurice Arnoux, Montrouge 92120, France.
authors.forename: E
authors.initials: E
authors.lastname: Soheili Majd
authors.forename: M
authors.initials: M
authors.lastname: Goldberg
authors.forename: L
authors.initials: L
authors.lastname: Stanislawski
mesh_terms: D000535:Aluminum / Q000032:analysis / Q000633:toxicity; D001205:Ascorbic Acid / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002839:Chromans / Q000494:pharmacology*; D003764:Dental Materials / Q000737:chemistry / Q000633:toxicity*; D003793:Dental Restoration, Permanent; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D005881:Gingiva / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000032:analysis / Q000633:toxicity; D008353:Mannitol / Q000494:pharmacology; D008422:Materials Testing; D008670:Metals / Q000032:analysis / Q000633:toxicity
chemical_list: D002839:Chromans; D003764:Dental Materials; D016166:Free Radical Scavengers; D008670:Metals; D008353:Mannitol; D000535:Aluminum; D007501:Iron; D005978:Glutathione; D001205:Ascorbic Acid; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid