title: Mitochondrial deletions in luteinized granulosa cells as a function of age in women undergoing in vitro fertilization.
abstract: OBJECTIVE
  To test the hypothesis that mitochondrial DNA (mtDNA) deletions are more prevalent in granulosa cells from women of advanced reproductive age than from younger women undergoing IVF.
  
  
  METHODS
  Granulosa cells screened for presence or absence of the 4977-bp deletion in human mtDNA.
  
  
  METHODS
  University-based fertility clinic.
  
  
  METHODS
  Twenty-four women divided equally between two groups: </=34 years old and >/=38 years old.
  
  
  METHODS
  Patients were given gonadotropin stimulation in preparation for IVF with granulosa cells isolated at the time of follicular aspiration.
  
  
  METHODS
  Presence or absence of the 4977-bp deletion in human mtDNA.
  
  
  RESULTS
  Seven out of 12 women analyzed who were <38 years old and 0 out of 12 women who were >38 years old had normal mtDNA as indicated by the presence of the 4977-bp fragment.
  
  
  CONCLUSIONS
  These data suggest that women over the age of 38 have granulosa cells that contain a substantial decrease in the level of normal mitochondria as compared with women </=34 years.
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, Division of Reproductive Endocrinology and Infertility, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901, USA. seiferdb@umdnj.edu
authors.forename: David B
authors.initials: DB
authors.lastname: Seifer
authors.forename: Vimaris
authors.initials: V
authors.lastname: DeJesus
authors.forename: Karen
authors.initials: K
authors.lastname: Hubbard
mesh_terms: D000328:Adult; D000375:Aging / Q000502:physiology*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D005307:Fertilization in Vitro; D017353:Gene Deletion; D006107:Granulosa Cells / Q000502:physiology*; D006801:Humans; D034461:Luteinization
chemical_list: D004272:DNA, Mitochondrial