Pyrococcus furiosus ferredoxin is a functional dimer.
Pyrococcus furiosus ferredoxin is subject to a monomer/dimer equilibrium as a function of ionic strength. At physiological ionic strength, approximately 0.35 M NaCl, the protein is very predominantly homodimer. The monomeric form exhibits impaired electron transfer on glassy carbon; it also has a decreased S=3/2 over S=1/2 ratio as shown by electron paramagnetic resonance spectroscopy. Even following sterilization at 121 degrees C the dimer is stable in denaturing gel electrophoresis.
Hasan
M N
MN
Kluyver Department of Biotechnology, Delft University of Technology, Delft, The Netherlands. m.n.hasan@tnw.tudelft.nl
Hagedoorn
P L
PL
Hagen
W R
WR
D002850:Chromatography, Gel; D019281:Dimerization; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport; D004591:Electrophoresis, Polyacrylamide Gel; D005288:Ferredoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology*; D009994:Osmolar Concentration; D020027:Pyrococcus furiosus
D005288:Ferredoxins