Identification and characterization of UEV3, a human cDNA with similarities to inactive E2 ubiquitin-conjugating enzymes.
Recent studies have shown that ubiquitination is an essential factor in endosomal sorting and virus assembly. The human TSG101 gene has been demonstrated to belong to a group of genes coding for apparently inactive E2 ubiquitin-conjugating enzymes, which exert regulatory effects on E2 activity in cellular ubiquitination processes. In this study, a novel human cDNA (UEV3) encoding a putative protein of 379 amino acids was isolated from a human placenta library that may represent a partial paralogue of human TSG101. The predicted protein contains an N-terminal domain homologous to the catalytic domain of ubiquitin-conjugating enzymes (Ubc), which is fused to a sequence showing significant homology to members of the lactate dehydrogenase protein family. The UEV3 gene is located on chromosome 11 closely adjacent to TSG101 and LDH-C. Northern blot and UEV3-specific reverse transcription/polymerase chain reaction (RT/PCR) analyses of various colon carcinoma cell lines as well as both normal and tumor samples from colon revealed an expression of the UEV3 cDNA in all tested samples.
Kloor
Matthias
M
Department of Molecular Pathology, University of Heidelberg, Im Neuenheimer Feld 220/221, Heidelberg, Germany.
Bork
Peer
P
Duwe
Alexander
A
Klaes
Ruediger
R
von Knebel Doeberitz
Magnus
M
Ridder
Ruediger
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002880:Chromosomes, Human, Pair 11; D018076:DNA, Complementary / Q000032:analysis* / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics; D056827:Endosomal Sorting Complexes Required for Transport; D015723:Gene Library; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000235:genetics; D008025:Ligases / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics; D014407:Tumor Cells, Cultured; D025801:Ubiquitin / Q000378:metabolism*; D044763:Ubiquitin-Conjugating Enzymes; D000099177:Tsg101 Protein
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D008025:Ligases; D014157:Transcription Factors; D025801:Ubiquitin; D044763:Ubiquitin-Conjugating Enzymes; D000099177:Tsg101 Protein; C409673:lactate dehydrogenase C4; C470840:UEVLD protein, human