Ranking the toxicity of fatty acids on Jurkat and Raji cells by flow cytometric analysis.
The fatty acids have an important role in the control of leukocyte metabolism and function. Higher concentrations of certain fatty acids, particularly polyunsaturated fatty acids (PUFAs) and volatile fatty acids, can cause cell death via apoptosis or, when concentrations are greater, necrosis. In this study, we determined the highest concentrations of various fatty acids that are non-toxic to two human leukemic cell lines, Jurkat (T-lymphocyte) and Raji (B-lymphocyte). Toxicity was evaluated by either loss of membrane integrity and/or DNA fragmentation using flow cytometric analysis. There were no remarkable differences for the toxicity of the fatty acids between B and T cell lines. The cytotoxicity of the fatty acids was related to the carbon chain length and number of double bonds: docosahexaenoic acid=eicosapentaenoic acid=arachidonic acid=gamma-linolenic acid=stearic acid=palmitic acid > linoleic acid=palmitoleic acid > vacenic acid=lauric acid > oleic acid > elaidic acid > capric acid > butyric acid > caprylic acid=caproic acid=propionic acid. The proportion of cells undergoing apoptosis or necrosis, induced by the fatty acids tested, remains to be investigated.
Lima
T M
TM
Thais Martins de Lima, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1524, 05508-900, Butantã, São Paulo, SP, Brazil. thais_lima@hotmail.com
Kanunfre
C C
CC
Pompéia
C
C
Verlengia
R
R
Curi
R
R
D017209:Apoptosis; D004249:DNA Damage; D005231:Fatty Acids, Unsaturated / Q000633:toxicity*; D005232:Fatty Acids, Volatile / Q000633:toxicity*; D005434:Flow Cytometry; D006801:Humans; D019169:Jurkat Cells; D007938:Leukemia / Q000473:pathology; D009336:Necrosis; D012016:Reference Values; D014407:Tumor Cells, Cultured
D005231:Fatty Acids, Unsaturated; D005232:Fatty Acids, Volatile