[Effects of mechanical strain on the proliferation of pulmonary epithelial cells and reorganization of integrins].
Using a cyclic strain unit, the proliferation and reorganization of alpha(5), beta(1 ) integrins in human pulmonary epithelial A549 cells that underwent mechanical strain were studied. Flow cytometry revealed that cellular proliferative index reduced significantly after cells were subjected to 15% elongation at frequency 20 cycles/min or 40 cycles/min for 48 h. Confocal microscopy revealed that alpha(5), beta(1 ) integrins in human pulmonary epithelial A549 cells transferred from the apic al layer to the basal layer and created adhesion plaques after 24 h exposure to 15% elongation at frequency 40 cycles/min. It is concluded that alpha(5), beta(1 ) integrins in pulmonary epithelial cells play an important role in transducing mechanical stress into intracellular signals.
Zhang
Hui-Jing
HJ
College of Bioengineering, Key Laboratory of Biomechanics Tissue Engineering under the State Ministry of Education, Chongqing University, Chongqing 400044, China. Zhanghuijing@mail.tmmu.com.cn
Cai
Shao-Xi
SX
Lu
Xiao
X
Wang
Yuan-Liang
YL
Wang
Hong-Bing
HB
Huang
Qi-Ping
QP
D002455:Cell Division / Q000502:physiology; D004847:Epithelial Cells / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D039482:Integrin alpha5 / Q000502:physiology; D019012:Integrin beta1 / Q000502:physiology; D016023:Integrins / Q000502:physiology*; D008168:Lung / Q000166:cytology / Q000502:physiology; D018613:Microscopy, Confocal; D013314:Stress, Mechanical; D014407:Tumor Cells, Cultured
D039482:Integrin alpha5; D019012:Integrin beta1; D016023:Integrins