title: Determination of total antioxidant capacity in plasma by cyclic voltammetry. Two case reports.
abstract: The cyclic voltammetry (CV) was used for the measurement of the plasma total antioxidant capacity from two types of patients. The first one consisted of 29 volunteers (men aged 18-21 years) who were administered placebo or silymarin at a dose of 858 mg/day. After two months of silymarine administration, CV revealed a statistically significant increase in total antioxidant capacity compared to placebo. No statistically significant changes in TBARS, SH-groups, creatininin, urea, and uric acid concentrations were found. The second group under study comprised 49 patients with chronic renal disease during dialysis therapy. After dialysis, CV revealed a decrease of total antioxidant capacity in the plasma, which was equivalent to a decrease in creatinine, urea and uric acid. CV was performed using a system consisting of a working glassy carbon electrode, an auxiliary platinum electrode, and a reference saturated calomel electrode; a linear change of voltage of 200 mV/s was applied. CV is a simple and relatively reliable method for assessment of body antioxidant status. It is also time and cost effective.
authors.affiliation: Institute of Medical Chemistry and Biochemistry, Palacký University, 775 15 Olomouc, Czech Republic. psotova@tunw.upol.cz
authors.forename: J
authors.initials: J
authors.lastname: Psotová
authors.forename: J
authors.initials: J
authors.lastname: Zahálková
authors.forename: J
authors.initials: J
authors.lastname: Hrbác
authors.forename: V
authors.initials: V
authors.lastname: Simánek
authors.forename: J
authors.initials: J
authors.lastname: Bartek
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D001769:Blood / Q000378:metabolism*; D002908:Chronic Disease; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D007674:Kidney Diseases / Q000097:blood* / Q000628:therapy; D008297:Male; D012016:Reference Values; D006435:Renal Dialysis
chemical_list: D000975:Antioxidants