Regulative effect of IFN-gamma on the Fas/Fas L system of cholangiocarcinoma cells.
OBJECTIVE
To detect the regulative effects of IFN-gamma on the expression of Fas and FasL of cholangiocarcinoma cells.


METHODS
We studied that the expression of Fas and FasL gene by the human cholangiocarcinoma cell line QBC939 by RT-PCR, Western blot, immunohistochemistry. At the same time, we investigated the regulative effect of IFN-gamma on them.


RESULTS
Fas and FasL mRNA and protein were expressed by cholangiocarcinoma cells. We also found IFN-gamma could upregulate the expression of the two genes (P < 0.01). However, IFN-gamma could also downregulate the ability of them to make Jurkat cells apoptotic. With the increasing of dosage and time, the effect was enhanced.


CONCLUSIONS
IFN-gamma could regulate the expression of Fas and FasL by cholangiocarcinoma cells, therefore it could reduce the ability of cholangiocarcinoma to occur immune escape. This provides new theoretical basis for immunological therapy of cholangiocarcinoma.
Li
Ziyu
Z
Department of General Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Wang
Jianming
J
Tang
Cong
C
Wang
Xin
X
Zou
Shengquan
S
D017209:Apoptosis / Q000187:drug effects; D001650:Bile Duct Neoplasms; D001653:Bile Ducts, Intrahepatic; D018281:Cholangiocarcinoma; D006801:Humans; D008562:Membrane Glycoproteins; D012333:RNA, Messenger / Q000235:genetics; D019014:fas Receptor
D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D019014:fas Receptor