Proteolytic processing of the hepatitis B virus e antigen precursor. Cleavage at two furin consensus sequences.
The Hepatitis B virus P22 protein is a nonstructural protein that is the precursor of the 17-kDa secreted e antigen (HBeAg). The mature HBeAg is obtained after the removal of the C-terminal region of P22, a process which involves a proprotein convertase. Our studies show first that the protease could cleave P22 at the C-terminal side of Arg(167) or Arg(154) and second, that the maturation process can be either done in one step or in two steps with the generation of a processing intermediate (P20). Our data also demonstrate that the removal of the P22 C terminus, which occurs mainly in the trans-Golgi network, can also be achieved after exocytosis. Keeping in mind this characteristic and the amino acid sequence of the cleavage sites, we concluded that furin is involved in the maturation of the HBeAg. In addition, we show that in our experimental system, the HBeAg is a 164-amino acid protein and not a 159-amino acid protein as previously reported.
Messageot
Fabienne
F
Laboratoire de Génétique des Virus, Gif sur Yvette, 91198 France.
Salhi
Samia
S
Eon
Patricia
P
Rossignol
Jean-Michel
JM
D000595:Amino Acid Sequence; D045683:Furin; D006513:Hepatitis B e Antigens / Q000096:biosynthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D043484:Proprotein Convertases; D011498:Protein Precursors / Q000378:metabolism*; D013381:Subtilisins / Q000502:physiology*
D006513:Hepatitis B e Antigens; D011498:Protein Precursors; D043484:Proprotein Convertases; D013381:Subtilisins; D045683:Furin