[Effect of glucose on the antibiotic activity and antibiotic resistance of Streptomyces peucetius subsp. caesius ATCC 27952-2 and its mutants].
The growth of anthracycline producer Streptomices peucetius subsp. caesius ATCC 27952-2 was inhibited by presence of glucose on complete media, containing alternative carbon sources. Amount of clones not producing antibiotic increased to 80.2 per cent along with elevation of glucose concentration in corn meal medium from 0.1 to 1.0 per cent. Mutants of S. peucetius subsp. caesius ATCC 27952-2 able to grow on complete media with 2 per cent of glucose (glr-mutants) were obtained. Glr-mutants had decreased antibiotic production in comparison with 27952-2 strain. 17 per cent of studied glr-mutants synthesized 1.6-3.1-fold quantities of anthracyclines in comparison with parental strain. Glr-mutants synthesized more biomass, although more slowly utilized glucose than strain 27952-2.
Dubitskaia
L P
LP
I. Franko Lviv University, Ukraine.
Fedorenko
V A
VA
D000903:Antibiotics, Antineoplastic / Q000378:metabolism* / Q000494:pharmacology*; D018533:Biomass; D003470:Culture Media; D003864:Depression, Chemical; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D009154:Mutation; D013302:Streptomyces / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
D000903:Antibiotics, Antineoplastic; D003470:Culture Media; D005947:Glucose