Effect of transforming growth factor-beta(2) on extracellular matrix synthesis in bovine trabecular meshwork cells.
OBJECTIVE
To investigate the effect of transforming growth factor-beta(2) (TGF-beta(2)) in human aqueous humor on extracellular matrix synthesis in cultured bovine trabecular meshwork cells.


METHODS
Cultured 3-5 passage bovine trabecular meshwork cells were divided into control group and experimental group. After treated with 0 ng/L (control), 0.32 x 10(3) ng/L, 1.00 x 10(3) ng/L, 3.20 x 10(3) ng/L TGF-beta(2) for 48 h, collagen, fibronectin and hyaluronic acid synthesis in bovine trabecular meshwork cells were examined respectively by (3)H-proline incorportion and liquid scintillation technique, enzyme linked immunosorbent assay (ELISA) and radioimmunoassay (RIA).


RESULTS
In comparison with the control group, TGF-beta(2) significantly promoted the synthesis of collagen at 0.32 x 10(3) ng/L (P < 0.05), 1.00 x 10(3) ng/L (P < 0.01), 3.20 x 10(3) ng/L (P < 0.01), (3)H-proline incorporation increased in a concentration-dependent manner; and 1.00 x 10(3) ng/L (P < 0.05), 3.20 x 10(3) ng/L (P < 0.01) TGF-beta(2) significantly promoted the synthesis of fibronectin in cultured bovine trabecular meshwork cells. 1.00 x 10(3) ng/L (P < 0.01) and 3.20 x 10(3) ng/L (P < 0.01) TGF-beta(2) significantly inhibited the synthesis of hyaluronic acid in the cultured cells.


CONCLUSIONS
The data suggest that TGF-beta(2) play important roles in extracellular matrix age-related changes of normal trabecular meshwork and abnormal deposition of the extracellular matrix in the trabecular meshwork, especially the juxtacanalicular tissue of primary open-angle glaucoma patients.
Cao
Yang
Y
Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China (Email: ytsao@sohu.com)
Wei
Houren
H
Zhang
Ying
Y
Da
Banghong
B
Lu
Yinping
Y
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism; D005902:Glaucoma, Open-Angle / Q000378:metabolism; D006801:Humans; D014129:Trabecular Meshwork / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000378:metabolism; D053781:Transforming Growth Factor beta2 / Q000494:pharmacology; D015291:Transforming Growth Factors
D016212:Transforming Growth Factor beta; D053781:Transforming Growth Factor beta2; D015291:Transforming Growth Factors