Tumor antigen peptides from HL-60 cells induce specific immune response.
OBJECTIVE
To prepare tumor antigen peptides from HL-60 cells and to induce specific immune response.


METHODS
HL-60 antigen peptides were obtained using techniques including freezing and thawing, heat precipitation and acid precipitation. The stimulating effect of the in vitro Hsp70 binding HL-60 peptides on PBMC and the proliferation of stimulated PBMC were observed by T cell activation test. The cytotoxicity of proliferated PBMC is detected by incubating HL-60 cells or K562 cells with PBMC respectively.


RESULTS
The obtained tumor antigen peptides were a peptides mixture. The mixed peptides could activate PBMC and cause PBMC proliferation in vitro after presented by Hsp70. The proliferated PBMC showed specific cytotoxicity to HL-60 cells but not to K562 cells.


CONCLUSIONS
The method for preparing of human leukemia tumor antigen peptides used in this paper is simple and easy; the obtained antigen peptides can induce specific immune response in vitro.
Huang
Bo
B
Department of Medical Molecular Biology, Tongji Medical College, Wuhan 430030, China.
Feng
Zuohua
Z
Zhang
Guimei
G
D002455:Cell Division; D018922:HL-60 Cells; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D009363:Neoplasm Proteins / Q000276:immunology*; D010455:Peptides / Q000276:immunology*
D018840:HSP70 Heat-Shock Proteins; D009363:Neoplasm Proteins; D010455:Peptides