Effects of probucol in hyperlipidemic rabbit liver: a preliminary ultrastructural study.
Probucol is a lipid-lowering agent with an antioxidant effect; however, its influence on the liver remains unclear. The effects of probucol on hyperlipidemic rabbit liver are investigated to add a structural data on its therapeutical profile. Local albino rabbits were divided into three groups. 1) Hyperlipidemic group: fed with 1% cholesterol (150 g/kg/day) enriched chow for 2 months. 2) Probucol treated group: group 1 + intraperitoneal probucol (10 mg/kg/day) administration for 15 days. 3) Control group fed with normal chow. The blood lipid profile was investigated biochemically. Liver samples were examined electronmicroscopically. Within the parenchymal cells of group 1, the amount of rough surfaced endoplasmic reticulum was increased, its cisterna was dilated displaying a moderately electron dense substance in it and showed close apposition with the condensed mitochondria. In group 2, smooth surfaced endoplasmic reticulum was in extensive amounts filling almost all of the cytoplasm, displayed a reticular, degenerated appearance and was in close relation with the condensed, degenerated mitochondria. Probucol may cause degenerative changes on the liver parenchyme at the subcellular level. It alters the structure of these cells mainly acting on the smooth surfaced endoplasmic reticulum and the mitochondria that are known to be involved in cellular detoxification.
Korkusuz
Petek
P
Hacettepe University, Faculty of Medicine, Department of Histology and Embryology. petek@harlequin.mc.metu
Ugur
Yeşim
Y
Aşan
Esin
E
Aktuna Keskil
Zuhal
Z
Uluoglu
Canan
C
D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology*; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005260:Female; D006949:Hyperlipidemias / Q000188:drug therapy* / Q000473:pathology*; D008099:Liver / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D011341:Probucol / Q000494:pharmacology*; D011817:Rabbits; D014617:Vacuoles / Q000648:ultrastructure
D000924:Anticholesteremic Agents; D011341:Probucol