Hydrophilic carboxylic acids and iridoid glycosides in the juice of American and European cranberries (Vaccinium macrocarpon and V. oxycoccos), lingonberries (V. vitis-idaea), and blueberries (V. myrtillus).
Analysis of the hydrophilic fraction of cranberry juice by reversed-phase HPLC using an Aqua LUNA column with diode array or MS detection revealed the presence of quinic acid, malic acid, shikimic acid, and citric acid. For the first time, two iridoid glucosides were found in the juice. The two iridoid glucosides were shown to be monotropein and 6,7-dihydromonotropein by MS and NMR spectroscopy. A fast reversed-phase HPLC method for quantification of the hydrophilic carboxylic acids was developed and used for analyses of cranberry, lingonberry, and blueberry juices. The level of hydrophilic carboxylic acids in cranberries was 2.67-3.57% (w/v), in lingonberries 2.27-3.05%, and in blueberries 0.35-0.75%. In lingonberries both iridoid glucosides were present, whereas only monotropein was present in blueberries.
Jensen
Heidi D
HD
Department of Medicinal Chemistry, Royal Danish School of Pharmacy, Copenhagen, Denmark.
Krogfelt
Karen A
KA
Cornett
Claus
C
Hansen
S Honoré
SH
Christensen
S Brøgger
SB
D001628:Beverages / Q000032:analysis*; D002264:Carboxylic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D019343:Citric Acid / Q000032:analysis; D039823:Iridoids / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008293:Malates / Q000032:analysis; D013058:Mass Spectrometry; D011801:Quinic Acid / Q000032:analysis; D012765:Shikimic Acid / Q000032:analysis; D029799:Vaccinium macrocarpon / Q000737:chemistry*; D029800:Vaccinium myrtillus / Q000737:chemistry*; D029801:Vaccinium vitis-idaea / Q000737:chemistry*
D002264:Carboxylic Acids; D039823:Iridoids; D008293:Malates; D011801:Quinic Acid; D019343:Citric Acid; D012765:Shikimic Acid; C030298:malic acid