title: Characterization of CMY-type beta-lactamases in clinical strains of Proteus mirabilis and Klebsiella pneumoniae isolated in four hospitals in the Paris area.
abstract: We isolated five clinical strains (three Proteus mirabilis and two Klebsiella pneumoniae) with beta-lactam resistance phenotypes consistent with production of an AmpC-type beta-lactamase. The predicted amino acid sequences of the enzymes were typical of class C beta-lactamases. The enzymes were identified as CMY-2, CMY-4 and a new CMY-variant beta-lactamase, CMY-12. The AmpC beta-lactamases from the two K. pneumoniae isolates were found to be encoded on self-transferable plasmids. The genes encoding the AmpC-type beta-lactamase produced by the three P. mirabilis isolates were chromosomal. Four of the five clinical isolates were from patients transferred from Greece, Algeria and Egypt; one of the K. pneumoniae strains was recovered from a French patient. PFGE analysis and rep-PCR fingerprinting showed that the two P. mirabilis isolates from Greek patients were closely related.
authors.affiliation: Service de Bactériologie, Hôpital Saint Antoine,UFR Saint-Antoine, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France. dominique.decre@sat.ap-hop-paris.fr
authors.forename: Dominique
authors.initials: D
authors.lastname: Decré
authors.forename: Charlotte
authors.initials: C
authors.lastname: Verdet
authors.forename: Laurent
authors.initials: L
authors.lastname: Raskine
authors.forename: Hervé
authors.initials: H
authors.lastname: Blanchard
authors.forename: Béatrice
authors.initials: B
authors.lastname: Burghoffer
authors.forename: Alain
authors.initials: A
authors.lastname: Philippon
authors.forename: Marie José
authors.initials: MJ
authors.lastname: Sanson-Le-Pors
authors.forename: Jean Claude
authors.initials: JC
authors.lastname: Petit
authors.forename: Guillaume
authors.initials: G
authors.lastname: Arlet
mesh_terms: D003428:Cross Infection / Q000201:enzymology* / Q000382:microbiology*; D006801:Humans; D007710:Klebsiella Infections / Q000201:enzymology / Q000453:epidemiology / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests / Q000706:statistics & numerical data; D010297:Paris / Q000453:epidemiology; D011512:Proteus Infections / Q000201:enzymology / Q000453:epidemiology / Q000382:microbiology; D011513:Proteus mirabilis / Q000201:enzymology / Q000302:isolation & purification*; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*
chemical_list: C103022:beta-lactamase CMY-2; D001618:beta-Lactamases