title: Cloning and sequencing of cytochrome P450 2X1 from channel catfish (Ictalurus punctatus).
abstract: Previous purification and immunochemical studies in livers of channel catfish indicated the presence of at least four cytochrome P450 (CYP) 2-like isoforms. Sequencing of the first 18 amino acids of one purified form indicated a CYP2 isoform. From this N-terminal sequence and other published CYP2 sequences from fish, primers were designed and a full-length CYP cDNA was identified from reverse-transcribed catfish liver mRNA. 5' and 3' RACE was used to obtain an open reading frame of 1470 bp encoding a 490 amino acid protein (approximately 57 kD). CYP2X1 was most identical to Fundulus heteroclitus CYP2P2 (41%); CYP2N2 (40%): and CYP2N1 (39%).
authors.affiliation: Department of Environmental Sciences, University of California, Riverside 92521, USA. daniel.schlenk@ucr.edu
authors.forename: D
authors.initials: D
authors.lastname: Schlenk
authors.forename: B
authors.initials: B
authors.lastname: Furnes
authors.forename: X
authors.initials: X
authors.lastname: Zhou
authors.forename: B C
authors.initials: BC
authors.lastname: Debusk
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D023781:Fundulidae / Q000235:genetics; D007059:Ictaluridae; D008969:Molecular Sequence Data; D020033:Protein Isoforms; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D020033:Protein Isoforms; D003577:Cytochrome P-450 Enzyme System