title: Cloning and sequence analysis of HIV-1 gene fragments isolated by restriction digest polymerase chain reaction method.
abstract: OBJECTIVE: To clone and analyze the HIV-1gene fragments isolated by restriction digest polymerase chain reaction (RD-PCR). METHOD: All the HIV-1 gene fragments were divided into 10 subgroups and amplified by RD-PCR. The PCR products of each subgroup were purified and cloned into the T-vectors, then identified rapidly. The plasmids were extracted from positive clones and the target gene fragments were amplified and sequenced. RESULTS: Sequences analysis showed that all the fragments amplified were HIV gene. CONCLUSION: A method for cloning and identifying multiple fragments has been improved and used for restriction fragments.
authors.affiliation: Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Ma
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Song
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Wu
authors.forename: Qiu-Ye
authors.initials: QY
authors.lastname: Guo
authors.forename: Wen-Ling
authors.initials: WL
authors.lastname: Zheng