Biochemical and genetic evidence for the involvement of yeast Ypt6-GTPase in protein retrieval to different Golgi compartments.
Yeast Ypt6p, the homologue of the mammalian Rab6 GTPase, is not essential for cell viability. Based on previous studies with ypt6 deletion mutants, a regulatory role of the GTPase either in protein retrieval to the trans-Golgi network or in forward transport between the endoplasmic reticulum (ER) and early Golgi compartments was proposed. To assess better the primary role(s) of Ypt6p, temperature-sensitive ypt6 mutants were generated and analyzed biochemically and genetically. Defects in N-glycosylation of proteins passing the Golgi and of Golgi-resident glycosyltransferases as well as protein sorting defects in the trans-Golgi were recorded shortly after functional loss of Ypt6p. ER-to-Golgi transport and protein secretion were delayed but not interrupted. Mis-sorting of the vesicular SNARE Sec22p to the late Golgi was also observed. Combination of the ypt6-2 mutant allele with a number of mutants in forward and retrograde transport between ER, Golgi, and endosomes led to synthetic negative growth defects. The results obtained indicate that Ypt6p acts in endosome-to-Golgi, in intra-Golgi retrograde transport, and possibly also in Golgi-to-ER trafficking.
Luo
Zongli
Z
Max Planck Institute for Biophysical Chemistry, Department of Molecular Genetics, D-37070 Göttingen, Germany.
Gallwitz
Dieter
D
D004721:Endoplasmic Reticulum / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis; D006031:Glycosylation; D016695:Glycosyltransferases / Q000502:physiology; D006056:Golgi Apparatus / Q000378:metabolism*; D020559:Monomeric GTP-Binding Proteins / Q000502:physiology*; D009154:Mutation; D021381:Protein Transport; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013696:Temperature; D020691:rab GTP-Binding Proteins / Q000502:physiology
D006023:Glycoproteins; D029701:Saccharomyces cerevisiae Proteins; D016695:Glycosyltransferases; C060755:YPT1 protein, S cerevisiae; D020559:Monomeric GTP-Binding Proteins; C499307:YPT6 protein, S cerevisiae; D020691:rab GTP-Binding Proteins