title: Electrophoretically mediated microscale reaction of glycosidases: kinetic analysis of some glycosidases at the nanoliter scale.
abstract: Capillary electrophoresis (CE) is one of the extremely important analytical techniques known for its high sensitivity and resolution. We have investigated electrophoretically mediated microanalysis (EMMA) for the assay of some native glycosidases. Under optimized conditions, the enzymatic reactions of alpha-glucosidase, beta-galactosidase and beta-N-acetylglucosaminidase were carried out, and the Michaelis constants were obtained. The current method may have advantages over traditional assay methods, especially in terms of the amount of enzyme and substrate required for a reaction.
authors.affiliation: Mitsubishi Kagaku Institute of Life Sciences (MLTILS), Machida-shi, 194-8511, Tokyo, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Kanie
authors.forename: Osamu
authors.initials: O
authors.lastname: Kanie
mesh_terms: D000818:Animals; D001331:Automation; D002021:Buffers; D002417:Cattle; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008832:Microchemistry; D010710:Phosphates; D012440:Saccharomyces / Q000201:enzymology; D012680:Sensitivity and Specificity
chemical_list: D002021:Buffers; D010710:Phosphates; D006026:Glycoside Hydrolases