Ca2+/cAMP response element-binding protein (CREB)-dependent activation of Per1 is required for light-induced signaling in the suprachiasmatic nucleus circadian clock.
Light is a prominent stimulus that synchronizes endogenous circadian rhythmicity to environmental light/dark cycles. Nocturnal light elevates mRNA of the Period1 (Per1) gene and induces long term state changes, expressed as phase shifts of circadian rhythms. The cellular mechanism for Per1 elevation and light-induced phase advance in the suprachiasmatic nucleus (SCN), a process initiated primarily by glutamatergic neurotransmission from the retinohypothalamic tract, was examined. Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN. CRE-decoy ODN also blocked light-induced phase advances in vivo. Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA. Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.
Tischkau
Shelley A
SA
Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, B107 CLSL, 61801, USA.
Mitchell
Jennifer W
JW
Tyan
Sheue-Houy
SH
Buchanan
Gordon F
GF
Gillette
Martha U
MU
D000818:Animals; D002118:Calcium / Q000502:physiology*; D018797:Cell Cycle Proteins; D002940:Circadian Rhythm / Q000502:physiology*; D017362:Cyclic AMP Response Element-Binding Protein / Q000502:physiology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000502:physiology; D005786:Gene Expression Regulation; D018698:Glutamic Acid / Q000378:metabolism / Q000494:pharmacology; D008027:Light; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000502:physiology; D009687:Nuclear Proteins / Q000235:genetics*; D056950:Period Circadian Proteins; D051381:Rats; D020318:Rats, Long-Evans; D020218:Response Elements / Q000502:physiology; D013493:Suprachiasmatic Nucleus / Q000502:physiology*
D018797:Cell Cycle Proteins; D017362:Cyclic AMP Response Element-Binding Protein; D009687:Nuclear Proteins; C539023:Per1 protein, mouse; C539024:Per1 protein, rat; D056950:Period Circadian Proteins; D009569:Nitric Oxide; D018698:Glutamic Acid; D017868:Cyclic AMP-Dependent Protein Kinases; D002118:Calcium