title: Antioxidant and antiviral activities of Euphorbia thymifolia L.
abstract: The antioxidant and antiviral activities of Euphorbia thymifolia L. (Euphorbiaceae) were investigated in this study. The results showed that all of the fractions (MeOH, CHCl(3), EtOAc, n-butanol and water) and pure compounds (3-O-galloyl-4,6-(S)-HHDP-D-glucose, rugosin B and 1,3,4,6-tetra-O-galloyl-K-beta-D-glucose)tested possessed antioxidant activities, with the exception of the organic aqueous fraction in the anti-lipid and anti-superoxide formation assays. The range of IC(50) of anti-lipid formation, anti-superoxide formation and free radical scavenging assays for all fractions and pure compounds were 2.81-7.63, 0.03-2.18 and 0.013-2.878 mg/ml, respectively. Electron spin resonance studies showed that water extract and pure compounds of E. thymifolia exhibited superoxide radical and hydroxyl radical scavenging activities. Besides antioxidant activities, 3-O-galloyl-4,6-(S)-HHDP-D-glucose and EtOAc fraction also showed anti-HSV-2 activity. Thus, E. thymifolia was concluded to possess antioxidant and anti-HSV-2 activities.
authors.affiliation: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC. aalin@ms24.hinet.net
authors.forename: Chun-Ching
authors.initials: CC
authors.lastname: Lin
authors.forename: Hua-Yew
authors.initials: HY
authors.lastname: Cheng
authors.forename: Chien-Min
authors.initials: CM
authors.lastname: Yang
authors.forename: Ta-Chen
authors.initials: TC
authors.lastname: Lin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005591:Chemical Fractionation; D028481:Euphorbia / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005609:Free Radicals; D018258:Herpesvirus 2, Human / Q000187:drug effects; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides
chemical_list: D000975:Antioxidants; D000998:Antiviral Agents; D016166:Free Radical Scavengers; D005609:Free Radicals; D010936:Plant Extracts; D013481:Superoxides