Expression and clinical implication of soluble resistance-associated calcium-binding protein gene and multi-drug resistance gene in leukemia.
OBJECTIVE
To study the relationship between soluble resistance-related calcium-binding protein (sorcin) gene and multidrug resistance gene (mdr1), and their significance in clinical drug resistance and prognosis of acute myeloid leukemia (AML).


METHODS
Amplification of sorcin gene and mdr1 gene in K562/A02 cell detected by Northern blot, were monitored by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) in 65 AML patients and 27 normal controls, with their relationship and clinical outcame analyzed.


RESULTS
The amplification of sorcin gene and mdr1 gene in AML patients were significantly higher than that in the normal control, which were related to clinical drug resistance and prognosis. The amplification of sorcin gene was related to the amplification of mdr1 gene in the two groups. The clinical drug resistance incidence rate and complete remission rate were 92.9% and 7.1% in sorcin(+)/mdr1(+) group. They were 8.6% and 91.4% in the sorcin(-)/mdr1(-) group (P < 0.001).


CONCLUSIONS
The co-amplification of sorcin and mdr1 gene can be taken as a good indicator of clinical drug resistance and prognosis of AML.
Li
Guangyao
G
National Laboratory of Experimental Hematology, Hematologic Institute, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin 300020, China.
Tan
Yaohong
Y
Yang
Chunzheng
C
Zhao
Chunhua
C
Zhao
Hongguo
H
Wang
Jianxiang
J
Xue
Yanping
Y
Han
Mingzhe
M
Qian
Linsheng
L
Zhao
Chunting
C
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics*; D000208:Acute Disease; D015152:Blotting, Northern / Q000379:methods; D002135:Calcium-Binding Proteins / Q000235:genetics*; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D015870:Gene Expression; D006801:Humans; D020014:K562 Cells; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000503:physiopathology; D009363:Neoplasm Proteins / Q000235:genetics*; D011379:Prognosis
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002135:Calcium-Binding Proteins; D009363:Neoplasm Proteins; C503113:SRI protein, human