title: PCR-based detection of Bacillus anthracis in formalin-fixed tissue from a patient receiving ciprofloxacin.
abstract: We demonstrate that Bacillus anthracis may be detected from a formalin-fixed, paraffin-embedded biopsy specimen, even after the patient has received antibiotic treatment. Although traditional PCR methods may not be sufficiently sensitive for anthrax detection in such patients, cycle numbers can be increased or PCR can be repeated by using an aliquot from a previous PCR as the template.
authors.affiliation: Departments of Medicine. Microbiology. Pathology, New York University School of Medicine. Veterans Affairs Medical Center, New York, New York 10016, USA.
authors.forename: Steven M
authors.initials: SM
authors.lastname: Levine
authors.forename: Guillermo
authors.initials: G
authors.lastname: Perez-Perez
authors.forename: Asalia
authors.initials: A
authors.lastname: Olivares
authors.forename: Herman
authors.initials: H
authors.lastname: Yee
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Hanna
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Blaser
mesh_terms: D000328:Adult; D000881:Anthrax / Q000188:drug therapy* / Q000382:microbiology; D000890:Anti-Infective Agents / Q000627:therapeutic use*; D001408:Bacillus anthracis / Q000187:drug effects / Q000302:isolation & purification*; D001706:Biopsy; D002939:Ciprofloxacin / Q000627:therapeutic use*; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D000890:Anti-Infective Agents; D005404:Fixatives; D005557:Formaldehyde; D002939:Ciprofloxacin