Differential expression of collagen IV alpha1 to alpha6 chains in basement membranes of benign and malignant odontogenic tumors.
Type IV collagen, the major component of basement membrane (BM), demonstrates a stage- and position-specific distribution of its isoforms during tooth development. To determine its localization in BM of odontogenic neoplasms, immunohistochemistry using six anti-alpha(IV) chain-specific monoclonal antibodies was performed. Results disclosed that BM demonstrated an irregular alpha(IV) chain profile in malignant odontogenic tumors as compared to benign odontogenic neoplasms. No alpha3(IV) chains were detected. Expression of alpha1(IV)/alpha2(IV) and alpha5(IV)/alpha6(IV) chains was stronger in desmoplastic ( n=3) than in ordinary (n=5) ameloblastomas. The adenomatoid odontogenic tumor ( n=2) distinctly expressed these chains in BM of cribriform areas and hyaline materials (which was also alpha4(IV)-positive), but weakly around epithelial whorls/rosettes/nests and mineralized foci. These five chains also stained BM and tumor cells of ameloblastic fibroma ( n=3) and ameloblastic fibro-odontosarcoma (n=1), but not the inductive hard tissues. Ameloblastic carcinoma ( n=2) showed specific alpha1(IV)/alpha2(IV) chain loss, while primary intraosseous carcinoma ( n=1) demonstrated a discontinuous alpha1(IV)/alpha2(IV) and alpha5(IV)/alpha6(IV) staining pattern. The present results suggest that modification and remodeling of BM collagen IValpha chains occur during odontogenic neoplasms' progression.
Nagatsuka
Hitoshi
H
Department of Oral Pathology and Medicine, Graduate School of Medicine and Dentistry, Okayama University, 2-5-1 Shikata-cho, Okayama 700-8525, Japan. jin@md.okayama-u.ac.jp
Siar
Chong Huat
CH
Nakano
Keisuke
K
Tsujigiwa
Hidetsugu
H
Gunduz
Mehmet
M
Choufuku
Hoh
H
Lee
You-Jin
YJ
Naito
Ichiro
I
Sado
Yoshikazu
Y
Nagai
Noriyuki
N
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism* / Q000473:pathology; D024141:Collagen Type IV / Q000096:biosynthesis* / Q000145:classification; D006801:Humans; D007124:Immunoenzyme Techniques; D007573:Jaw Neoplasms / Q000378:metabolism* / Q000473:pathology; D009808:Odontogenic Tumors / Q000145:classification / Q000378:metabolism* / Q000473:pathology; D051381:Rats
D024141:Collagen Type IV