Preclinical qualitative evaluation of the antitumoral pharmacodynamic action of some natural polyphenolic biopreparations.
We have investigated the impact of POLYAS I and POLYAS II polyphenolic biopreparations - specifically separated and purified from Asclepias syriaca leaves, and characterized in vitro as cytotoxic and/or cytostatic agents - on the tumor generation process. A series of in vivo tests of their effect on the development of Guerin T-8 lymphotropic epithelioma and Walker 256 carcinosarcoma were conducted. In a first stage of preclinical trial we had used several tests meant to evaluate their antitumoural activity indices. The same tests were then used under similar experimental conditions in the solid tumoral systems mentioned. A comparative analysis of the antitumoral activity evaluation indices resulting from our tests with the reference indices set by the American and German preclinical screening programs pointed to their compatibility. Thus, we found similar values of mean tumoral regressions, of the ratio between mean tumoral weights of the treated and control groups, respectively, of the T/C products resulting from successive re-tests. Also T/C values resulting from retests were within the limits of admissible variability range. All those results highlighted the antineoplastic pharmacotherapeutic effect of the polyphenolic biopreparations and also proved that effect to be replicable. The qualitative evaluation of the pharmacodynamic action of those preparations was a condition for their further quantitative pharmacological evaluation in point of antitumoral therapeutic effectiveness in a preclinical stage.
Rotinberg
P
P
Institute of Biological Research, Jassy, Romania.
Kelemen
S
S
Gramescu
M
M
Rotinberg
H
H
Nuta
V
V
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D031108:Asclepias; D002279:Carcinoma 256, Walker / Q000188:drug therapy*; D005069:Evaluation Studies as Topic; D005260:Female; D005419:Flavonoids; D009374:Neoplasms, Experimental / Q000188:drug therapy*; D010636:Phenols / Q000494:pharmacology*; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D059808:Polyphenols; D051381:Rats; D017208:Rats, Wistar
D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D010636:Phenols; D028321:Plant Preparations; D011108:Polymers; D059808:Polyphenols