title: [Nuclear factor-kappa B and intercellular adhesion molecule-1 expression in airway epithelial cells from smoking rats].
abstract: OBJECTIVE
  To investigate the changes and of nuclear factor-kappa B (NF-kappaB) and intercellular adhesion molecule (ICAM)-1 expression in airway epithelial cells from smoking rats.
  
  
  METHODS
  Wistar rats (n = 39) were randomly divided into a nonsmoking control group and 2 smoking groups-smoking for 1 month and 3 months, with 13 rats in each group. Immunohistochemistry and in situ hybridization staining were used to semiquantitatively analyse the changes of NF-kappaB and ICAM-1 expression in airway epithelial cells.
  
  
  RESULTS
  (1) The percentages of positive cells for NF-kappaB nuclear staining in bronchiolar epithelial cells in 1 month smoking group (63 +/- 4)% and 3 month smoking group [(51 +/- 5)%] were significantly higher than that in the control group [(27 +/- 5)%] (P < 0.01 respectively). (2) ICAM-1 mRNA expression in bronchial and bronchiolar epithelial cells in the 1 month smoking group (0.645 +/- 0.038, 0.747 +/- 0.041) and the 3 month smoking group (0.688 +/- 0.062, 0.809 +/- 0.023) was significantly higher than those in the control group (0.526 +/- 0.023, 0.635 +/- 0.044), (P < 0.01 respectively), and the expression of ICAM-1 mRNA in the 3 month smoking group was higher than that in the 1 month smoking group (P < 0.05 and P < 0.01 respectively). (3) ICAM-1 protein expression in the main bronchial and bronchiolarepithelial cells in the 1 month smoking group (0.73 +/- 0.04, 0.94 +/- 0.05) and the 3 month smoking group (0.77 +/- 0.04, 0.99 +/- 0.03) was significantly higher than those in the control group (0.57 +/- 0.04, 0.83 +/- 0.04) (P < 0.01 respectively), and the expression of ICAM-1 protein in the 3 month smoking group was higher than those in the 1 month smoking group (P < 0.05 respectively). (4) ICAM-1 mRNA and protein expression in bronchioles were significantly higher than those in the bronchi (P < 0.01 respectively) in all groups. (5) The percentage of cells staining positive for nuclear NF-kappaB correlated with ICAM-1 protein expression in bronchioles from the 1 month smoking group (r = 0.462, P < 0.05).
  
  
  CONCLUSIONS
  NF-kappaB and ICAM-1 may play an important role in smoking-induced airway inflammation.
authors.affiliation: Department of Pulmonary Disease, Tongji Hospital, Tongji Medical College, Wuhan 430030, China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Xu
authors.forename: Yongcheng
authors.initials: Y
authors.lastname: Du
authors.forename: Dongyan
authors.initials: D
authors.lastname: Li
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000818:Animals; D001980:Bronchi / Q000737:chemistry / Q000378:metabolism*; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis* / Q000235:genetics; D008297:Male; D016328:NF-kappa B / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012907:Smoking / Q000378:metabolism*
chemical_list: D016328:NF-kappa B; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1