title: Type-1 transforming growth factor-beta differentially modulates tumoricidal activity of murine peritoneal macrophages against metastatic variants of the B16 murine melanoma.
abstract: Transforming growth factor-beta 1 (TGF-beta 1) renders mouse peritoneal macrophages tumoricidal against metastatic variants of the B16 mouse melanoma in vitro. Both direct cytotoxicity and indirect cytotoxicity were observed. A subthreshold concentration (10 U/ml) of recombinant murine interferon-gamma (rMuIFN-gamma) enhanced the direct tumoricidal activity of TGF-beta 1-activated macrophages from 29% to 88% but did not change their indirect tumoricidal profile. Data obtained from macrophages preincubated with either TGF-beta 1 or rMuIFN-gamma showed that TGF-b1 can initiate tumoricidal activity better than rMuIFN-gamma. These effects were plasma-membrane mediated because targeting macrophages with liposomal TGF-beta 1 was ineffective. The order of tumoricidal susceptibility of the B16 melanoma lines to activated macrophages was B16F1 > B16F10 > B16BL6, in inverse order of metastatic potential.
authors.affiliation: Department of Cancer Biology-173, University of Texas, M. D. Anderson Cancer Center, 1515 Holcomble Boulevard, Houston, Texas 77030, USA. dfan@mdanderson.org
authors.forename: Dominic
authors.initials: D
authors.lastname: Fan
authors.forename: Amy
authors.initials: A
authors.lastname: Liaw
authors.forename: Yvonne M
authors.initials: YM
authors.lastname: Denkins
authors.forename: James H
authors.initials: JH
authors.lastname: Collins
authors.forename: Melissa
authors.initials: M
authors.lastname: Van Arsdall
authors.forename: Jolie Lien
authors.initials: JL
authors.lastname: Chang
authors.forename: Subhas
authors.initials: S
authors.lastname: Chakrabarty
authors.forename: Dieuthu
authors.initials: D
authors.lastname: Nguyen
authors.forename: Ewa
authors.initials: E
authors.lastname: Kruzel
authors.forename: Isaiah J
authors.initials: IJ
authors.lastname: Fidler
mesh_terms: D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D008081:Liposomes; D017737:Macrophages, Peritoneal / Q000276:immunology*; D008546:Melanoma, Experimental / Q000276:immunology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1; D014407:Tumor Cells, Cultured
chemical_list: D008081:Liposomes; C508961:Tgfb1 protein, mouse; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1