title: The effect of a porcine-derived small intestinal submucosa product on wounds with exposed bone in dogs.
abstract: OBJECTIVE
  To determine the effect of a porcine-derived small intestinal submucosa product (PSIS) on healing time, epithelialization, angiogenesis, contraction, and inflammation of wounds with exposed bone on the distal aspect of the limbs of dogs.
  
  
  METHODS
  Prospective, controlled, experimental study.
  
  
  METHODS
  10 young adult, purpose-bred, male Beagles.
  
  
  METHODS
  Small wounds with exposed bone were created on the lateral aspect of metatarsal V and the medial aspect of metatarsal II on both hindlimbs. Three sheets of PSIS were sutured into the wounds of the treated limb, and the other limb served as a control. On day 10, punch biopsies of the medial metatarsal wounds were collected and were evaluated microscopically after routine hematoxylin and eosin and phosphotungstic acid hematoxylin (PTAH) staining. The lateral metatarsal wounds were evaluated by planimetry and laser Doppler perfusion imaging on days 7, 14, and 21. Time until complete wound healing was also recorded. The level of significance was set at P < or =.05 for all statistical analyses.
  
  
  RESULTS
  Laser Doppler perfusion measurements were significantly higher in control wounds on day 7, but no differences were noted on days 14 and 21. No significant differences in planimetric values, histopathologic appearance, or time until complete wound healing were noted among treated and control groups.
  
  
  CONCLUSIONS
  No objective differences in healing were noted between control wounds and wounds treated with PSIS.
  
  
  CONCLUSIONS
  There appears to be no contraindication to the use of PSIS on clean wounds with exposed bone on the distal limbs of dogs. However, our objective data provides no evidence that this product affects epithelialization, contraction, or time to complete healing in wounds with exposed bone.
authors.affiliation: Scott Ritchey Research Center and the Department of Clinical Sciences, Auburn University, AL 36849, USA.
authors.forename: James T
authors.initials: JT
authors.lastname: Winkler
authors.forename: Steven F
authors.initials: SF
authors.lastname: Swaim
authors.forename: Eva A
authors.initials: EA
authors.lastname: Sartin
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Henderson
authors.forename: Janet A
authors.initials: JA
authors.lastname: Welch
mesh_terms: D000818:Animals; D001684:Biological Dressings / Q000662:veterinary*; D004285:Dogs / Q000293:injuries* / Q000601:surgery*; D006614:Hindlimb; D007413:Intestinal Mucosa / Q000637:transplantation; D017078:Laser-Doppler Flowmetry; D007869:Leg Injuries / Q000601:surgery / Q000662:veterinary*; D008297:Male; D012867:Skin / Q000098:blood supply* / Q000293:injuries; D013552:Swine; D016896:Treatment Outcome; D014945:Wound Healing