title: Increased urinary hydrogen peroxide levels caused by coffee drinking.
abstract: Experiments with volunteers in Singapore have demonstrated that coffee drinking increases urinary hydrogen peroxide levels (Long, Halliwell, Free Rad. Res., 32, 463-467 (2000)). We re-examined the effect of coffee drinking of healthy Japanese subjects on urinary hydrogen peroxide levels. A cup of brewed or canned coffee commercially available in Japan generated 120-420 micro mol hydrogen peroxide in incubation in a neutral medium at 37 degrees C for 6 h. The increased levels were higher than those obtained from a cup of green tea extract or a glass of red wine. After the subject drank a cup of coffee, apparent hydrogen peroxide levels (micro mol/g creatinine) in urine collected 1-2 h after coffee drinking increased 3-10-fold compared to the levels before coffee drinking. The increased urinary hydrogen peroxide levels are likely derived mainly from 1,2,4-benzenetriol excreted in urine, because the major component that generates hydrogen peroxide is found to be 1,2,4-benzenetriol, and storing urine collected after coffee drinking increased hydrogen peroxide levels in a time-dependent fashion. Total hydrogen peroxide equivalent levels excreted in 3 h-urine after coffee drinking were estimated to be 0.5-10% that of coffee consumed. A residual amount of hydrogen peroxide may be retained or consumed in human bodies.
authors.affiliation: School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hiramoto
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kida
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Kikugawa
mesh_terms: D000328:Adult; D000368:Aged; D003069:Coffee / Q000378:metabolism*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000652:urine*; D008297:Male; D008875:Middle Aged; D013997:Time Factors
chemical_list: D003069:Coffee; D006861:Hydrogen Peroxide