title: IgE-mediated hypersensitivity against human sweat antigen in patients with atopic dermatitis.
abstract: Sweating aggravates itch in atopic dermatitis, but the mechanism is unclear. In this study, we examined the involvement of type I hypersensitivity in the aggravation of atopic dermatitis by sweating. Skin tests with autologous sweat were positive in 56 of 66 patients (84.4%) with atopic dermatitis, but only in 3 of 27 healthy volunteers (11.1%). Sweat samples from both patients and healthy volunteers induced varying degrees of histamine release from basophils of patients with atopic dermatitis. However, the histamine release was impaired by removal of IgE on the basophils. Incubation of basophils with myeloma IgE before sensitization with serum of patients blocked the ability to release histamine-induced sweat. IgE antibody against antigen(s) in sweat may be present in serum of patients with atopic dermatitis. Key words:
authors.affiliation: Department of Dermatology, Graduate School of Biomedical Sciences, Hiroshima University, Japan. mhide@hiroshima-u.ac.jp
authors.forename: Michihiro
authors.initials: M
authors.lastname: Hide
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Tanaka
authors.forename: Yumi
authors.initials: Y
authors.lastname: Yamamura
authors.forename: Osamu
authors.initials: O
authors.lastname: Koro
authors.forename: Shoso
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D001324:Autoantigens / Q000032:analysis; D001491:Basophils / Q000276:immunology / Q000378:metabolism; D016022:Case-Control Studies; D002478:Cells, Cultured; D015331:Cohort Studies; D003876:Dermatitis, Atopic / Q000276:immunology* / Q000503:physiopathology; D003888:Desensitization, Immunologic; D005260:Female; D006632:Histamine / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology* / Q000503:physiopathology; D007073:Immunoglobulin E / Q000032:analysis*; D008297:Male; D011336:Probability; D012016:Reference Values; D012680:Sensitivity and Specificity; D012882:Skin Tests; D013542:Sweat / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D001324:Autoantigens; D007073:Immunoglobulin E; D006632:Histamine