title: Identification of SNARE and cell trafficking regulatory proteins in the salivary glands of the lone star tick, Amblyomma americanum (L.).
abstract: Prostaglandin E(2) (PGE(2)) stimulates secretion of tick salivary gland proteins via a phosphoinositide signaling pathway and mobilization of intracellular Ca(2+) (). Highly conserved intracellular SNARE (soluble NSF attachment protein receptors) complex proteins are associated with the mechanism of protein secretion in vertebrate and invertebrate neuronal and non-neuronal cells. Proteins in the salivary glands of partially fed female lone star ticks cross-react individually with antibodies to synaptobrevin-2 (vesicle (v)-SNARE), syntaxin-1A, syntaxin-2 and SNAP-25 (target (t)-SNAREs), cytosolic alpha/beta SNAP and NSF (N-ethylmaleimide-sensitive fusion protein), Ca(2+) sensitive synaptotagmin, vesicle associated synaptophysin, and regulatory cell trafficking GTPases Rab3A and nSec1. V-SNARE and t-SNARE proteins form an SDS-resistant, boiling sensitive core complex in the salivary glands. Antibodies to SNARE complex proteins inhibit PGE(2)-stimulated secretion of anticoagulant protein in permeabilized tick salivary glands. We conclude that SNARE and cell trafficking regulatory proteins are present and functioning in the process of PGE(2)-stimulated Ca(2+) regulated protein secretion in tick salivary glands.
authors.affiliation: Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater 74078, USA.
authors.forename: S
authors.initials: S
authors.lastname: Karim
authors.forename: R C
authors.initials: RC
authors.lastname: Essenberg
authors.forename: J W
authors.initials: JW
authors.lastname: Dillwith
authors.forename: J S
authors.initials: JS
authors.lastname: Tucker
authors.forename: A S
authors.initials: AS
authors.lastname: Bowman
authors.forename: J R
authors.initials: JR
authors.lastname: Sauer
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D002463:Cell Membrane Permeability; D017124:Conserved Sequence; D015232:Dinoprostone / Q000494:pharmacology; D019476:Insect Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D050600:SNARE Proteins; D012469:Salivary Glands / Q000187:drug effects / Q000502:physiology*; D013987:Ticks / Q000502:physiology*; D033921:Vesicular Transport Proteins
chemical_list: D019476:Insect Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050600:SNARE Proteins; D033921:Vesicular Transport Proteins; D015232:Dinoprostone