title: Cloning and characterization of two novel crystal protein genes from a Bacillus thuringiensis serovar dakota strain.
abstract: Two genes encoding the 32- and 40-kDa polypeptides of Bacillus thuringiensis strain 90-F-45-14 crystals were cloned, expressed in an acrystalliferous B. thuringiensis strain, and sequenced. The polypeptides had deduced molecular weights of 30,319 and 33,885, respectively. The amino acid sequence of the 32-kDa protein was 37.7% identical to the known sequence of a non-insecticidal parasporal protein in B. thuringiensis serovar thompsoni crystals. The sequence of the cloned 40-kDa protein was 37.0% and 22.3% identical to that of the existing Cry protein classes, Cry15Aa1 and Cry23Aa1, respectively. Thus, this protein constitutes a novel protein class, Cry33Aa1. The open reading frames of the two genes were located on the predominant plasmid of 17,629 bp (=11,752 MDa) in the same orientation, and they were separated by the sequence of 32 nucleotides. The two proteins are likely produced simultaneously from a single transcript to form spherical crystals.
authors.affiliation: Biotechnology & Food Research Institute, Fukuoka Industrial Technology Center, Aikawa-machi 1465-5 Kurume, Fukuoka 839-0861, Japan.
authors.forename: Ho San
authors.initials: HS
authors.lastname: Kim
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Saitoh
authors.forename: Satoko
authors.initials: S
authors.lastname: Yamashita
authors.forename: Tetsuyuki
authors.initials: T
authors.lastname: Akao
authors.forename: Yu Shin
authors.initials: YS
authors.lastname: Park
authors.forename: Minoru
authors.initials: M
authors.lastname: Maeda
authors.forename: Rie
authors.initials: R
authors.lastname: Tanaka
authors.forename: Eiichi
authors.initials: E
authors.lastname: Mizuki
authors.forename: Michio
authors.initials: M
authors.lastname: Ohba
mesh_terms: D000595:Amino Acid Sequence; D001413:Bacillus thuringiensis / Q000166:cytology / Q000235:genetics; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D001427:Bacterial Toxins; D001483:Base Sequence; D003001:Cloning, Molecular; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D008957:Models, Genetic; D013171:Spores, Bacterial / Q000648:ultrastructure
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis