title: Identification of snails within the Bulinus africanus group from East Africa by multiplex SNaPshot trade mark analysis of single nucleotide polymorphisms within the cytochrome oxidase subunit I.
abstract: Identification of populations of Bulinus nasutus and B. globosus from East Africa is unreliable using characters of the shell. In this paper, a molecular method of identification is presented for each species based on DNA sequence variation within the mitochondrial cytochrome oxidase subunit I (COI) as detected by a novel multiplexed SNaPshotTM assay. In total, snails from 7 localities from coastal Kenya were typed using this assay and variation within shell morphology was compared to reference material from Zanzibar. Four locations were found to contain B. nasutus and 2 locations were found to contain B. globosus. A mixed population containing both B. nasutus and B. globosus was found at Kinango. Morphometric variation between samples was considerable and UPGMA cluster analysis failed to differentiate species. The multiplex SNaPshotTM assay is an important development for more precise methods of identification of B. africanus group snails. The assay could be further broadened for identification of other snail intermediate host species.
authors.affiliation: Wolfson Wellcome Biomedical Laboratories, Department of Zoology, The Natural History Museum, London.
authors.forename: J R
authors.initials: JR
authors.lastname: Stothard
authors.forename: J
authors.initials: J
authors.lastname: Llewellyn-Hughes
authors.forename: C E
authors.initials: CE
authors.lastname: Griffin
authors.forename: S J
authors.initials: SJ
authors.lastname: Hubbard
authors.forename: T K
authors.initials: TK
authors.lastname: Kristensen
authors.forename: D
authors.initials: D
authors.lastname: Rollinson
mesh_terms: D000818:Animals; D001483:Base Sequence; D002033:Bulinus / Q000033:anatomy & histology / Q000201:enzymology / Q000235:genetics*; D016000:Cluster Analysis; D003576:Electron Transport Complex IV / Q000235:genetics*; D014644:Genetic Variation; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012553:Schistosomiasis haematobia / Q000235:genetics*
chemical_list: D003576:Electron Transport Complex IV