Immobilization of natural macromolecules on poly-L-lactic acid membrane surface in order to improve its cytocompatibility.
With the use of a grafting-coating method, three kinds of natural macromolecules, that is, gelatin, collagen, or chitosan, were immobilized on poly-L-lactic acid (PLLA) membrane surfaces with the goal of improving of cellular interactions. Attenuated total reflectance infrared spectroscopy (ATR-IR), x-ray photoelectron spectroscopy (XPS) and surface morphology analysis using scanning electronic microscopy (SEM) confirmed that the natural macromolecule layers adhered tightly to the hydrophobic PLLA membrane surfaces. Chondrocyte culture showed that the modified PLLA membranes had higher cell attachment, higher cell proliferation rate, and higher cell activity than the control PLLA membrane. Moreover, the chondrocytes were more spread out on the modified PLLA membranes than on the control PLLA membranes.
Ma
Zuwei
Z
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Gao
Changyou
C
Gong
Yihong
Y
Ji
Jian
J
Shen
Jiacong
J
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D019902:Chondrocytes / Q000166:cytology; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D003094:Collagen; D005780:Gelatin; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000138:chemical synthesis / Q000737:chemistry; D046911:Macromolecular Substances; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011091:Polyesters; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000138:chemical synthesis / Q000737:chemistry; D011817:Rabbits; D013499:Surface Properties; D013778:Tetrazolium Salts; D013844:Thiazoles
D020099:Coated Materials, Biocompatible; D046911:Macromolecular Substances; D011091:Polyesters; D011108:Polymers; D013778:Tetrazolium Salts; D013844:Thiazoles; D002686:Chitin; D019344:Lactic Acid; C033616:poly(lactide); D005780:Gelatin; D003094:Collagen; D019904:Polymethyl Methacrylate; D048271:Chitosan; C022616:thiazolyl blue