title: The gene CSTF2T, encoding the human variant CstF-64 polyadenylation protein tauCstF-64, lacks introns and may be associated with male sterility.
abstract: Messenger RNA polyadenylation in male germ cells does not seem to require the AAUAAA polyadenylation signal required in all other cell types. To account for this difference, we found a variant form of the polyadenylation protein, the 64,000 Mr protein of the cleavage stimulation factor (CstF-64), in mouse meiotic and postmeiotic germ cells. This protein is a candidate to alter polyadenylation in those cells. More recently, we reported the cloning from mouse pachytene spermatocytes of mouse tauCstF-64 (gene symbol Cstf2t), which is a homolog of CstF-64 fitting the criteria we expected for the variant CstF-64 protein. Here we report the cloning and mapping of the human ortholog of mouse tauCstF-64. The human tauCstF-64 cDNA (gene symbol CSTF2T) is 2324 bp in length and encodes a protein of 616 amino acids (64,442.90 Da). Although most highly related to mouse tauCstF-64 (89.8% identity), human tauCstF-64 is also related to the human and mouse somatic CstF-64 (74.9% and 73.4% identity, respectively). Alignment of human tauCstF-64 with human genome sequence from chromosome 10 shows that CSTF2T lacks introns. Radiation hybrid mapping places the human tauCstF-64 gene at 10q22-q23, which is the site of a translocation that has been associated with human neurological problems and male infertility.
authors.affiliation: Department of Cell Biology & Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas, 79430, USA.
authors.forename: Brinda
authors.initials: B
authors.lastname: Dass
authors.forename: Lisa
authors.initials: L
authors.lastname: McDaniel
authors.forename: Roger A
authors.initials: RA
authors.lastname: Schultz
authors.forename: Eman
authors.initials: E
authors.lastname: Attaya
authors.forename: Clinton C
authors.initials: CC
authors.lastname: MacDonald
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D039241:Cleavage Stimulation Factor; D014644:Genetic Variation; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D011061:Poly A; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D013090:Spermatocytes / Q000378:metabolism*; D014178:Translocation, Genetic
chemical_list: C474466:CSTF2T protein, human; D039241:Cleavage Stimulation Factor; D016601:RNA-Binding Proteins; D011061:Poly A