Sponge (2',5')oligoadenylate synthetase activity in the whole sponge organism and in a primary cell culture.
A high (2',5')oligoadenylate (2-5A) synthetase activity was found in the marine sponge Geodia cydonium. Here we demonstrate that the 2-5A synthetase activity is present also in other sponge species although the level of the 2-5A synthetase activity varies in several magnitudes in different sponges. The 2-5A synthesizing activity was maintained in the primary culture produced from a sponge.
Kelve
Merike
M
Laboratory of Molecular Genetics, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618, Tallinn, Estonia. merike@kbfi.ee
Kuusksalu
Anne
A
Lopp
Annika
A
Reintamm
Tõnu
T
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism*; D000818:Animals; D002457:Cell Extracts / Q000032:analysis; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods*; D004789:Enzyme Activation; D008386:Marine Biology / Q000379:methods; D011161:Porifera / Q000145:classification / Q000201:enzymology*; D012016:Reference Values; D012623:Seawater; D013045:Species Specificity; D013696:Temperature
D002457:Cell Extracts; D015088:2',5'-Oligoadenylate Synthetase