title: Immunohistochemical localization of active caspase-3 in the mouse ovary: growth and atresia of small follicles.
abstract: Caspase-3 belongs to a family of highly conserved cysteine proteases that mediate the course of apoptotic cell suicide. It is recognized that ovarian follicular atresia is associated with apoptosis, a process that has been characterized mainly in larger antral follicles. The aims of this study were to investigate the expression of caspase-3 in the mouse ovary, and determine whether active caspase-3 is present within smaller follicles, which may constitute the resting pool. The inactive enzyme was expressed as a 32 kDa band on a western blot of tissue extracts, whereas the active form was localized immunohistochemically. Bromodeoxyuridine (BrdU) was administered to mice (n = 7) during a 12 h period and subsequently localized to identify potentially quiescent follicles. Measurements of BrdU-positive cells in the mouse ovary were extrapolated with data obtained by morphometric analyses of small follicles using the nucleator technique. BrdU was incorporated into the granulosa cells of follicles regardless of size and the number of cells they contained, but was absent in a large proportion (89%) of small, single layered follicles. Active caspase-3 was localized to both the oocyte and granulosa cells of follicles that were considered to be undergoing atresia, but was not localized to the granulosa cells of any small, single layered follicles. The results of this study indicate that, in small follicles, granulosa cell proliferation occurs independently of the size of follicles and the number of constituent cells, and that follicles of this type may be inherently less susceptible to the normal physiological factors that induce atresia.
authors.affiliation: Department of Anatomy and Structural Biology, University of Otago, School of Medical Sciences, PO Box 913, Dunedin, New Zealand.
authors.forename: M A
authors.initials: MA
authors.lastname: Fenwick
authors.forename: P R
authors.initials: PR
authors.lastname: Hurst
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D053148:Caspase 3; D020169:Caspases / Q000032:analysis*; D002455:Cell Division; D004789:Enzyme Activation; D005260:Female; D005496:Follicular Atresia / Q000502:physiology*; D006107:Granulosa Cells / Q000201:enzymology; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D009865:Oocytes / Q000201:enzymology; D006080:Ovarian Follicle / Q000502:physiology*
chemical_list: C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases