Ischemic preconditioning attenuates the oxidant-dependent mechanisms of reperfusion cell damage and death in rat liver.
In an in vivo rat model of liver ischemia followed by reperfusion a consistent appearance of necrosis and activation of biochemical pathways of apoptosis was reproduced and monitored after 30 minutes reperfusion. Preconditioning by application of a short cycle of ischemia-reperfusion (10 minutes + 10 minutes) positively conditioned recovery of the organ at reperfusion, attenuating both necrotic and apoptotic events. Preconditioning at least halved cell oxidative damage occurring early at reperfusion, and as a major consequence, the increase of cytolysis and apoptosis occurring at reperfusion was about 50% less. The attenuation of both pathways of cell death by preconditioning appeared at least partly related to its modulate action on H(2)O(2) and 4-hydroxy-2,3-trans-nonenal production. The overall data point to a marked diminished oxidant generation and oxidative reactions as one major possible mechanism through which ischemic preconditioning exerts protection against necrotic and apoptotic insult to the postischemic liver.
Cavalieri
Barbara
B
Laboratory of Experimental Liver Pathology, the Department of Clinical and Biological Sciences, University of Turin, Italy.
Perrelli
Maria-Giulia
MG
Aragno
Manuela
M
Mastrocola
Raffaella
R
Corvetti
Giovanna
G
Durazzo
Marilena
M
Poli
Giuseppe
G
Cutrìn
Juan C
JC
D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D015536:Down-Regulation; D006861:Hydrogen Peroxide / Q000378:metabolism; D019194:Ischemic Preconditioning; D008099:Liver / Q000473:pathology* / Q000503:physiopathology*; D008102:Liver Circulation; D008297:Male; D009336:Necrosis; D016877:Oxidants / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology* / Q000503:physiopathology*
D000447:Aldehydes; D003574:Cytochrome c Group; D016877:Oxidants; C462359:tert-4-hydroxy-2-nonenal; D006861:Hydrogen Peroxide; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases