Observation of protein-derived (BSA) oxygen-centered radicals by EPR spin-trapping techniques.
Electron paramagnetic resonance (EPR) spin-trapping experiments, employing the novel spin-trap DEPMPO, provide evidence for the formation of protein-peroxyl radicals from the reaction of bovine serum albumin (BSA) or lysozyme with HO. in the presence of O2. Spin-trapping leads to the detection of anisotropic spectra of partially immobilized protein-peroxyl spin-adducts; positive identification is based on a novel spectrum simulation approach (through which broadened anisotropic spectra are simulated and compared with experiment) and by comparison of results with those obtained when MeO2. is trapped and the adduct frozen in a solid matrix.
Clément
Jean-Louis
JL
Laboratoire SREP UMR 6517, CNRS et Universit d'Aix-Marseille 1 et 3, France. jlclement@srepir1.univ-mrs.fr
Gilbert
Bruce C
BC
Rockenbauer
Antal
A
Tordo
Paul
P
Whitwood
Adrian C
AC
D000818:Animals; D016880:Anisotropy; D002417:Cattle; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D009113:Muramidase / Q000737:chemistry; D010100:Oxygen; D012709:Serum Albumin / Q000737:chemistry; D012984:Software
D005609:Free Radicals; D012709:Serum Albumin; D006861:Hydrogen Peroxide; D009113:Muramidase; D010100:Oxygen