Detection of oxazolidinone-resistant Enterococcus faecalis and Enterococcus faecium strains by real-time PCR and PCR-restriction fragment length polymorphism analysis.
A real-time PCR assay identified linezolid-resistant Enterococcus faecalis and Enterococcus faecium isolates with a G2576U rRNA mutation. PCR-restriction fragment length polymorphism analysis of ribosomal DNA amplicons with NheI also detected this mutation. Both assays detected isolates heterozygous at this position. Recognition of isolates with what is presently the most frequent oxazolidinone resistance mutation may aid surveillance and individual case management.
Woodford
Neil
N
Antibiotic Resistance Monitoring and Reference Laboratory. Laboratory of Enteric Pathogens, Central Public Health Laboratory, London NW9 5HT, United Kingdom. nwoodford@phls.org.uk
Tysall
Luke
L
Auckland
Cressida
C
Stockdale
Mark W
MW
Lawson
Andrew J
AJ
Walker
Rachel A
RA
Livermore
David M
DM
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004275:DNA, Ribosomal / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics; D016984:Enterococcus faecium / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D023303:Oxazolidinones / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics
D000900:Anti-Bacterial Agents; D004275:DNA, Ribosomal; D023303:Oxazolidinones; D012335:RNA, Ribosomal; C067043:endodeoxyribonuclease NheI; D015252:Deoxyribonucleases, Type II Site-Specific