title: Expression of adenosine triphosphate P2X3 receptors in rat molar pulp and trigeminal ganglia.
abstract: OBJECTIVE
  The objective of this study was to investigate the expression of adenosine triphosphate P2X3 receptor subunits in rat molar pulp and trigeminal ganglia and their relationship to substance P.
  
  
  METHODS
  Rat molar pulp and trigeminal ganglia were fixed and sectioned. Double immunostaining with anti-P2X3 and anti-substance P were used to localize P2X3 and substance P expression simultaneously with different stains.
  
  
  RESULTS
  P2X3 immunoreactivity (IR) fibers were present in root and coronal pulp. P2X3-IR fibers formed subodontoblastic plexus and advanced into the predentin and dentin. P2X3-IR neurons were localized in small and medium-sized cells in trigeminal ganglia. Colocalization of P2X3 and substance P was not found in either molar pulp or trigeminal ganglia.
  
  
  CONCLUSIONS
  The results of this study indicated that adenosine triphosphate in pulp tissues may stimulate a subpopulation of non-substance P trigeminal afferent fibers through activation of P2X3 receptors.
authors.affiliation: Department of Endodontics, College of Dentistry, University of Florida, Gainsville, FL 32610-0436, USA.
authors.forename: Jin
authors.initials: J
authors.lastname: Jiang
authors.forename: Jianguo
authors.initials: J
authors.lastname: Gu
mesh_terms: D000255:Adenosine Triphosphate / Q000502:physiology; D000818:Animals; D003782:Dental Pulp / Q000294:innervation* / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D009475:Neurons, Afferent; D051381:Rats; D018048:Receptors, Purinergic P2 / Q000096:biosynthesis*; D058477:Receptors, Purinergic P2X3; D013268:Stimulation, Chemical; D013373:Substance P / Q000032:analysis; D012668:Trigeminal Ganglion / Q000378:metabolism*
chemical_list: C546410:P2rx3 protein, rat; D018048:Receptors, Purinergic P2; D058477:Receptors, Purinergic P2X3; D013373:Substance P; D000255:Adenosine Triphosphate