Analysis of genetic polymorphisms at the interleukin-10 loci in aggressive and chronic periodontitis.
OBJECTIVE
Different cytokine genotypes have been described in periodontal disease. The aim of the present study was to investigate the genetic association of two previously described interleukin-10 (IL-10) polymorphisms in patients with aggressive (AP) and chronic periodontitis (CP) and to investigate possible associations with clinical manifestations.


METHODS
Based on clinical parameters and radiographs, 23 patients with CP and 18 patients with AP were included in the study. Additionally, 21 age-matched healthy subjects served as a control group. Genomic DNA was isolated from whole blood samples and the IL-10 promoter sequences from positions - 597 to - 824 were amplified by polymerase chain reaction (PCR). Polymorphisms were detected by restriction-enzyme cleavage. The A and C alleles at the - 597 position were associated with the T and C alleles at the - 824 position, respectively. Fisher's exact test was used for the statistical analysis.


RESULTS
No significant differences were observed in the allele frequencies between controls and AP patients (p = 0.70) or CP patients (p = 0.43), although the previously reported association between allele A at position - 597 and allele T at position - 824 was observed in our population.


CONCLUSIONS
We conclude that the investigated polymorphisms are not associated with periodontal disease.
Gonzales
J R
JR
Department of Periodontology and Department of Statistics, University of Giessen, Germany. jose.gonzales@dentist.med.uni-giessen.de
Michel
J
J
Diete
A
A
Herrmann
J M
JM
Bödeker
R H
RH
Meyle
J
J
D000208:Acute Disease; D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002878:Chromosomes, Human, Pair 1; D002908:Chronic Disease; D005787:Gene Frequency; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D010512:Periodontal Index; D010518:Periodontitis / Q000097:blood / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic
D016753:Interleukin-10