Glycosidase mechanisms.
The three-dimensional structure of glycosidases and of their complexes and the study of transition-state mimics reveal structural details that correlate with mechanism. Of particular interest are the transition-state conformations harnessed by individual enzymes and the substrate distortion observed in enzyme-ligand complexes. 3D-structure in synergy with transition-state mimicry opens the way for mechanistic interpretation of enzyme inhibition and for the development of therapeutic agents.
Vasella
Andrea
A
Laboratorium für Organische Chemie, ETH Hönggerberg, HCI H317, CH-8093 Zürich, Switzerland.
Davies
Gideon J
GJ
Böhm
Matthias
M
D002384:Catalysis; D004791:Enzyme Inhibitors / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis; D008024:Ligands; D015394:Molecular Structure; D016296:Mutagenesis; D011487:Protein Conformation; D013237:Stereoisomerism; D013379:Substrate Specificity
D004791:Enzyme Inhibitors; D008024:Ligands; D006026:Glycoside Hydrolases