title: Diversity of transconjugants that acquired plasmid pJP4 or pEMT1 after inoculation of a donor strain in the A- and B-horizon of an agricultural soil and description of Burkholderia hospita sp. nov. and Burkholderia terricola sp. nov.
abstract: We examined the diversity of transconjugants that acquired the catabolic plasmids pJP4 or pEMT1, which encode degradation of 2,4-dichlorophenoxyacetic acid (2,4-D), in microcosms with agricultural soil inoculated with a donor strain (Dejonghe, W., Goris, J., El Fantroussi, S., Höfte, M., De Vos, P., Verstraete, W., and Top, E. M. Appl. Environ. Microbiol. 2000, p. 3297-3304). Using repetitive element PCR fingerprinting, eight different rep-clusters and six separate isolates could be discriminated among 95 transconjugants tested. Representative isolates were identified using 16S rDNA sequencing, cellular fatty acid analysis, whole-cell protein analysis and/or DNA-DNA hybridisations. Plasmids pJP4 and pEMT1 appeared to have a similar transfer and expression range, and were preferably acquired and expressed in soil by indigenous representatives of Ralstonia and Burkholderia. Two rep-clusters were shown to represent novel Burkholderia species, for which the names Burkholderia hospita sp. nov. and Burkholderia terricola sp. nov. are proposed. When easily degradable carbon sources were added together with the plasmid-bearing donor strain, also a significant proportion of Stenotrophomonas maltophilia isolates were found. The transconjugant collections isolated from A- (0-30 cm depth) and B-horizon (30-60 cm depth) soil were similar, except for B. terricola transconjugants, which were only isolated from the B-horizon.
authors.affiliation: Laboratorium voor Microbiologie, Universiteit Gent, Belgium. johan.goris@rug.ac.be
authors.forename: Johan
authors.initials: J
authors.lastname: Goris
authors.forename: Winnie
authors.initials: W
authors.lastname: Dejonghe
authors.forename: Enevold
authors.initials: E
authors.lastname: Falsen
authors.forename: Elke
authors.initials: E
authors.lastname: De Clerck
authors.forename: Ben
authors.initials: B
authors.lastname: Geeraerts
authors.forename: Anne
authors.initials: A
authors.lastname: Willems
authors.forename: Eva M
authors.initials: EM
authors.lastname: Top
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
authors.forename: Paul
authors.initials: P
authors.lastname: De Vos
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000378:metabolism; D000383:Agriculture; D001426:Bacterial Proteins / Q000235:genetics; D020562:Betaproteobacteria / Q000145:classification / Q000235:genetics*; D001673:Biodegradation, Environmental; D019117:Burkholderia / Q000145:classification* / Q000235:genetics*; D003227:Conjugation, Genetic; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D018014:Gene Transfer Techniques; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism; D020588:Stenotrophomonas / Q000145:classification / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D015084:2,4-Dichlorophenoxyacetic Acid