title: Granulocyte responses to experimental injection of live and formalin-killed bacteria in carp (Cyprinus carpio).
abstract: The objective of the study was to investigate the dynamics of changes in number of granulocytes in bacterial infections of carp (Cyprinus carpio). Carp were inoculated with non-pathogenic or pathogenic bacteria and changes in type I (neutrophils) and type II granulocyte (basophils/eosinophils) counts in kidney, circulating blood and peritoneal cavity were assessed. After the injection of non-pathogenic bacteria (Escherichia coli), the number of type I and II cells in blood increased after 6-12h, but returned to the control level after 24-48 h. In contrast, after the injection of pathogenic bacteria (Aeromonas hydrophila), the number of type I cells initially increased followed by an increase in the number of type II cells. The peak counts of type I and II cells were at 12 and 24h after the injection, respectively. When the fish were given serial-injections of formalin-killed bacteria at 12-h intervals, the type II cells also predominantly increased and remained at high levels, following the peak count of type I cells.
authors.affiliation: Department of Veterinary Medicine, Nihon University, Kameino 1866, Fujisawa, Kanagawa, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kodama
authors.forename: Kiyohiko
authors.initials: K
authors.lastname: Tijiwa
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Moritomo
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nakanishi
mesh_terms: D016980:Aeromonas hydrophila / Q000166:cytology / Q000276:immunology*; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology; D002347:Carps / Q000276:immunology*; D004926:Escherichia coli / Q000166:cytology / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology; D005557:Formaldehyde; D006098:Granulocytes / Q000166:cytology / Q000276:immunology*; D007958:Leukocyte Count; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000276:immunology
chemical_list: D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated; D005557:Formaldehyde