title: Evidence for a Trypanosoma brucei lipoprotein scavenger receptor.
abstract: African trypanosomes are lipid auxotrophs that live in the bloodstream of their human and animal hosts. Trypanosomes require lipoproteins in addition to other serum components in order to multiply under axenic culture conditions. Delipidation of the lipoproteins abrogates their capacity to support trypanosome growth. Both major classes of serum lipoproteins, LDL and HDL, are primary sources of lipids, delivering cholesterol esters, cholesterol, and phospholipids to trypanosomes. We show evidence for the existence of a trypanosome lipoprotein scavenger receptor, which facilitates the endocytosis of both native and modified lipoproteins, including HDL and LDL. This lipoprotein scavenger receptor also exhibits selective lipid uptake, whereby the uptake of the lipid components of the lipoprotein exceeds that of the protein components. Trypanosome lytic factor (TLF1), an unusual HDL found in human serum that protects from infection by lysing Trypanosoma brucei brucei, is also bound and endocytosed by this lipoprotein scavenger receptor. HDL and LDL compete for the binding and uptake of TLF1 and thereby attenuate the trypanosome lysis mediated by TLF1. We also show that a mammalian scavenger receptor facilitates lipid uptake from TLF1 in a manner similar to the trypanosome scavenger receptor. Based on these results we propose that HDL, LDL, and TLF1 are all bound and taken up by a lipoprotein scavenger receptor, which may constitute the parasite's major pathway mediating the uptake of essential lipids.
authors.affiliation: Department of Medical and Molecular Parasitology, New York University School of Medicine, New York, New York 10010, USA.
authors.forename: Heather P
authors.initials: HP
authors.lastname: Green
authors.forename: Maria
authors.initials: M
authors.lastname: Del Pilar Molina Portela
authors.forename: Emmanuelle N
authors.initials: EN
authors.lastname: St  Jean
authors.forename: Elena B
authors.initials: EB
authors.lastname: Lugli
authors.forename: Jayne
authors.initials: J
authors.lastname: Raper
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology; D018955:CD36 Antigens / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D051379:Mice; D015800:Protozoan Proteins / Q000378:metabolism*; D018110:Receptors, Lipoprotein / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000166:cytology / Q000378:metabolism*
chemical_list: D018955:CD36 Antigens; D005456:Fluorescent Dyes; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D015800:Protozoan Proteins; D018110:Receptors, Lipoprotein; C434063:TLF1 protein, human