Evaluation of methods to increase the sensitivity and timeliness of detection of Streptococcus agalactiae in pregnant women.
Direct culture of rectovaginal specimens on Granada agar was compared to culture on sheep blood agar plate (SBAP) and AccuProbe detection of group B streptococcus from overnight LIM broth enhancement cultures (LIM-SBAP). Both broth-enhanced methods demonstrated excellent sensitivity (97.5% for LIM-SBAP and 93.5% for AccuProbe), while Granada agar demonstrated a sensitivity of only 40.3%.
Overman
Sue B
SB
University of Kentucky Hospital. Department of Pathology and Laboratory Medicine, University of Kentucky, Lexington, Kentucky 40536-0293, USA.
Eley
Douglas D
DD
Jacobs
Barry E
BE
Ribes
Julie A
JA
D000362:Agar; D001431:Bacteriological Techniques; D003470:Culture Media; D015342:DNA Probes / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D006801:Humans; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology*; D012335:RNA, Ribosomal / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D012007:Rectum / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000382:microbiology*; D013292:Streptococcus agalactiae / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D013997:Time Factors; D014621:Vagina / Q000382:microbiology*
D003470:Culture Media; D015342:DNA Probes; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D011933:Reagent Kits, Diagnostic; D000362:Agar