title: [Pathological research in experimental diabetes in rat].
abstract: OBJECTIVE
  To study dynamically the pathological changes of diabetic retinopathy (DR).
  
  
  METHODS
  The techniques of light microscopy, immunohistochemical staining, electron microscopy and histochemical electron microscopy were used to quantitatively analyze the Type IV collagen, laminin and their anionic sites in the rat retinal capillary basement membrane (RBM).
  
  
  RESULTS
  RBM became gradually thickened and was accompanied with an increased staining reaction for type IV collagen and laminin and decrease of anionic sites in number.
  
  
  CONCLUSIONS
  The increase in thickness, in type IV collagen and laminin and the decrease of the anionic sites in the RBM may be the pathological changes of the exudative lesion in DR.
authors.affiliation: Department of Ophthalmology, The second Affiliated Hospital, Hebei Medical University, Shijiazhuang 050000, China. wlg522@hotmail.com
authors.forename: Lingge
authors.initials: L
authors.lastname: Wei
authors.forename: Changling
authors.initials: C
authors.lastname: Wang
authors.forename: Huijun
authors.initials: H
authors.lastname: Duan
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D002196:Capillaries / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure; D024141:Collagen Type IV / Q000378:metabolism; D003930:Diabetic Retinopathy / Q000378:metabolism / Q000473:pathology*; D005260:Female; D007150:Immunohistochemistry; D007797:Laminin / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure; D013997:Time Factors
chemical_list: D024141:Collagen Type IV; D007797:Laminin