title: Seroconversion against Lutzomyia longipalpis saliva concurrent with the development of anti-Leishmania chagasi delayed-type hypersensitivity.
abstract: Antibody responses to salivary gland sonicate (SGS) from Lutzomyia longipalpis were investigated using serum samples from individuals living in an area where visceral leishmaniasis is endemic. Individuals were classified into 2 groups, according to the alteration of their responses to Leishmania chagasi antigen over the course of 6 months. Group 1 included children who experienced anti-L. chagasi seroconversion from negative to positive; group 2 included children who experienced delayed-type hypersensitivity (DTH) response to L. chagasi antigen conversion from negative to positive. Individuals who experienced seroconversion against L. chagasi antigens did not have increased anti-saliva antibody response, whereas those who developed a positive anti-L. chagasi DTH response had increased immunoglobulin (Ig) G, IgG1 and IgE anti-SGS antibody levels. Despite wide variation, serum samples from individuals in group 2 recognized more bands in SGS than did those from individuals in group 1. This simultaneous appearance of anti-saliva humoral response and anti-L. chagasi cell-mediated immunity supports the hypothesis that induction of immune response against SGS can facilitate induction of a protective response against leishmaniasis.
authors.affiliation: Centro de Pesquisa Gonçalo Moniz, Fundação Oswaldo Cruz, and Faculdade de Medicina, Universidade Federal da Bahia, Salvador, Bahia, Brazil.
authors.forename: Regis B
authors.initials: RB
authors.lastname: Gomes
authors.forename: Cláudia
authors.initials: C
authors.lastname: Brodskyn
authors.forename: Camila I
authors.initials: CI
authors.lastname: de Oliveira
authors.forename: Jackson
authors.initials: J
authors.lastname: Costa
authors.forename: José C
authors.initials: JC
authors.lastname: Miranda
authors.forename: Arlene
authors.initials: A
authors.lastname: Caldas
authors.forename: Jesus G
authors.initials: JG
authors.lastname: Valenzuela
authors.forename: Manoel
authors.initials: M
authors.lastname: Barral-Netto
authors.forename: Aldina
authors.initials: A
authors.lastname: Barral
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D002648:Child; D019353:Endemic Diseases; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D007891:Leishmania / Q000276:immunology*; D007896:Leishmaniasis / Q000453:epidemiology / Q000276:immunology*; D011576:Psychodidae / Q000276:immunology*; D012469:Salivary Glands / Q000276:immunology
chemical_list: D007074:Immunoglobulin G