Isolation and characterization of an infectious HIV type 1 molecular clone from a patient with primary infection.
We isolated a subtype B infectious DNA clone of human immunodeficiency virus type 1 (HIV-1) from a seronegative patient with acute infection and determined the entire nucleotide sequence. All the reading frames encoding the structural proteins (Gag, Pol, and Env) and nonstructural proteins (Tat, Rev, Vpr, Vif, and Nef) were found. Although moat functional domains in these proteins were conserved, we identified a duplication of the T cell factor lei (TCF-1alpha) element in the long terminal repeat and many variations in the N-linked glycosylation sites in the V4-V5 region but not in the V1-V3 loop of Env, compared with prototype subtype B clones. Furthermore, phylogenetic analysis of the entire nucleotide sequence indicated that this HIV-1 was distinct from the prototype subtype B clones, suggesting that transmitted viruses can be variants. This HIV-1 DNA done will be a useful prototype for investigating the mechanism of HIV-1 transmission.
Takeuchi
Hiroaki
H
Department of Virology, Tohoku University Graduate School of Medicine, Aoba-ku, Sendai 980-8575, Japan.
Suzuki
Youichi
Y
Tatsumi
Masashi
M
Hoshino
Hiroo
H
Daar
Eric S
ES
Koyanagi
Yoshio
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D015658:HIV Infections / Q000821:virology*; D018023:HIV Seronegativity; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D004279:DNA, Viral; D014764:Viral Proteins