title: Prognostic significance of laminin-5 gamma2 chain expression in colorectal carcinoma: immunohistochemical analysis of 103 cases.
abstract: OBJECTIVE
  The laminin-5 gamma2 chain plays an important role in cell migration during tumor invasion and tissue remodeling. Although this chain has been reported to be expressed in tumor-stroma interface of colorectal carcinoma, prognostic significance of its expression has not been elucidated in these tumors, so we investigated the clinicopathologic significance of Laminin-5 gamma2 chain expression in colorectal carcinoma.
  
  
  METHODS
  Laminin-5 gamma2 chain expression was investigated immunohistochemically in 103 colorectal carcinoma patients with Stage II, III, and IV disease. The patients were categorized into three groups according to the number of immunopositive tumor cells in the sections containing the maximum diameter of the tumor as follows: +, less than 20 tumor cells were positive; ++, 20 to 500 tumor cells were positive; +++, more than 500 tumor cells were positive.
  
  
  RESULTS
  Laminin-5 gamma2 chain expression was observed in cytoplasm of tumor cells, especially those in the invasive front of the tumor penetration. Eighteen (17 percent) of tumors showed +, 60 (58 percent) showed ++, and 25 (24 percent) showed +++. The increased number of immunopositive tumor cells was significantly associated with synchronous liver metastasis (P = 0.048). The univariate (P = 0.036) and multivariate (P = 0.001) analysis of the patients' survival revealed that the prognosis became significantly poorer in patients with the increased number of immunopositive tumor cells.
  
  
  CONCLUSIONS
  Increased laminin-5 gamma2 chain immunoreactivity, suggesting a high invasive potential of tumor cells, was a significant poor prognostic indicator for the patients with colorectal carcinoma.
authors.affiliation: Pathology Division, National Cancer Research Institute and Hospital, Tokyo, Japan.
authors.forename: Seishi
authors.initials: S
authors.lastname: Aoki
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Nakanishi
authors.forename: Shingo
authors.initials: S
authors.lastname: Akimoto
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Moriya
authors.forename: Kimio
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Masaki
authors.initials: M
authors.lastname: Kitajima
authors.forename: Michiie
authors.initials: M
authors.lastname: Sakamoto
authors.forename: Setsuo
authors.initials: S
authors.lastname: Hirohashi
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D000368:Aged; D000704:Analysis of Variance; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D015996:Survival Rate; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D000097794:Kalinin