Oligonucleotide microarray data mining: search for age-dependent gene expression.
Information on gene expression in colon tumors versus normal human colon was recently generated by an oligonucleotide microarray study. We used the associated database to search for genes that display age-dependent variations in expression. Statistically significant evidence was obtained that such genes are present in both the tumor and normal tissue databases. Besides the analysis of all genes included in the database, three subsets of genes were analyzed separately: genes controlled by p53, and genes coding for ribosomal proteins and for nuclear-encoded mitochondrial proteins. Among the genes controlled by p53 some show an age-dependent change in expression in tumor tissues, in the sense compatible with an activation of p53 at higher age. A decreased expression of some ribosomal genes at advanced age was detected both in tumor and normal tissues. No significant age-dependent expression could be detected for genes encoding mitochondrial proteins.
Kirschner
Marc
M
Carl C. Icahn Institute for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.
Pujol
Gemma
G
Radu
Aurelian
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000235:genetics*; D003106:Colon / Q000378:metabolism; D003110:Colonic Neoplasms / Q000235:genetics; D030561:Databases, Nucleic Acid; D020869:Gene Expression Profiling; D016158:Genes, p53; D006801:Humans; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics
D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins