title: Binding symmetry of extracellular divalent cations to conduction pore studied using tandem dimers of a CNG channel.
abstract: Cyclic nucleotide-gated (CNG) channels are composed of the tetramer of alpha-subunit alone or alpha- and beta-subunits. The alpha-subunits of these channels have a conserved glutamate (Glu) residue within the pore-forming region and the residue determines the selectivity as well as the affinity for the extracellular divalent cations. Using the high-affinity mutant (E363D) of bovine retinal CNG channel in which the Glu at position 363 was replaced to Asp, we constructed tandem dimers and investigated the binding characteristics of divalent cations to the site. The gating and permeation characteristics of individual homomeric tandem dimers are indistinguishable to those of homo-tetramers formed by parental monomers. The heteromeric tandem dimers showed the binding affinity for Sr(2+) identical to the geometric mean of the affinities for two parent channels, indicating the energy additive and thus the simultaneous interaction. On the other hand, the binding affinity for Mg(2+) followed the harmonic mean of those parent channels indicating that Mg(2+) interacts more strongly with the subunit bearing Asp residue at the position. Thus the results strongly suggest that the Glu363 residues in the CNG channel pore be flexible enough to adapt different binding symmetries for different divalent cations. Moreover, the simultaneous interaction between the four Glu residues and Sr(2+) provides an important structural constraint to the CNG channel outer vestibule of unknown structure.
authors.affiliation: Laboratory of Molecular Neurobiology, Department of Life Science, Kwangju Institute of Science and Technology (K-JIST), 1 Oryong-dong, Buk-gu, Gwangju 500-712, Republic of Korea.
authors.forename: Ryuk-Jun
authors.initials: RJ
authors.lastname: Kwon
authors.forename: Tal Soo
authors.initials: TS
authors.lastname: Ha
authors.forename: Wonjae
authors.initials: W
authors.lastname: Kim
authors.forename: Chul-Seung
authors.initials: CS
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002413:Cations, Divalent / Q000378:metabolism*; D054815:Cyclic Nucleotide-Gated Cation Channels; D019281:Dimerization; D005260:Female; D007473:Ion Channels / Q000737:chemistry / Q000378:metabolism*; D017136:Ion Transport; D008274:Magnesium / Q000378:metabolism; D008969:Molecular Sequence Data; D011485:Protein Binding; D013324:Strontium / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: D002413:Cations, Divalent; D054815:Cyclic Nucleotide-Gated Cation Channels; D007473:Ion Channels; D008274:Magnesium; D013324:Strontium