Localization of tumor by chemiluminescence probe during photosensitization action.
As a chemiluminescence (CL) probe, 3,7-dihydro-6-[4-[2-(N'-(5-fluoresceinyl) thioureido)ethoxy]phenyl]-2-methylimidazo[1,2-a]pyrazin-3-one dosium salt (FCLA) can sensitively and specifically react with singlet oxygen ((1)O(2)) and superoxide (O(2)(-)). Based on the fact that photosensitization action involves (1)O(2) generation, this report presents a novel method for localization of tumor mediated by FCLA. In experiments, FCLA was used as an optical reporter of (1)O(2) produced from photosensitization reaction of hematoporphyrin derivative. The effects of azide inhibition and deuterium enhancement on photosensitized CL indicated that (1)O(2) molecules generated during photosensitization action could be measured and imaged. In vivo experiments in vivo with tumor-bearing nude mice showed a clear CL image of tumor. The study suggests that CL imaging method may be applicable to the detection of (1)O(2) and the diagnosis of superficial tumors.
Wang
Juan
J
Institute of Laser Life Science, South China Normal University, 510631, Guangzhou, China.
Xing
Da
D
He
Yonghong
Y
Hu
Xuejin
X
D000818:Animals; D004195:Disease Models, Animal; D005452:Fluoresceins; D006801:Humans; D007093:Imidazoles; D008163:Luminescent Measurements; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009374:Neoplasms, Experimental / Q000175:diagnosis*; D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species; D026082:Singlet Oxygen / Q000032:analysis; D019810:Sodium Azide
D005452:Fluoresceins; D007093:Imidazoles; D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species; C080213:3,7-dihydro-6-(4-(2-(N'-(5-fluoresceinyl)thioureido)ethoxy)phenyl)-2-methylimidazo-(1,2-a)pyrazin-3-one; D026082:Singlet Oxygen; D019810:Sodium Azide