title: Evaluation of a rapid human immunodeficiency virus test at two community clinics in Kwazulu-Natal.
abstract: OBJECTIVE
  To establish whether the Determine (Abbott, Tokyo, Japan) HIV antibody test is suitable as an on-site rapid HIV test at primary health care centres by determining its sensitivity and specificity compared with the standard enzyme-linked immunoassay (ELISA) test.
  
  
  METHODS
  Prospective field evaluation study of a rapid HIV test compared with an ELISA.
  
  
  METHODS
  KwaDabeka clinic and St Mary's Hospital, urban primary health care clinics in the Durban western metropolitan area.
  
  
  METHODS
  Women attending antenatal clinics and those presenting at onset of labour.
  
  
  METHODS
  Performance of the rapid test versus conventional ELISA testing, sensitivity, specificity, feasibility of implementing the test at primary health care clinics, prevalence of HIV infection at study sites and its association with patient booking status.
  
  
  RESULTS
  A total of 323 specimens were tested from patients from two community clinics, KwaDabeka (N = 159) and St Mary's (N = 164). The overall HIV prevalence was 45.5%. There was a significant difference in HIV prevalence (P < 0.001) between KwaDabeka (35.2%) and St Mary's (55.5%). Of the participants 49.2% were from KwaDabeka clinic and 50.8% from St Mary's Hospital. Overall, HIV prevalence among unbooked participants was 43.0%, and among booked participants 46.3%. This was not statistically different (P = 0.612) between the two clinics. The rapid test showed a sensitivity and specificity of 100% when compared with a conventional diagnostic ELISA test.
  
  
  CONCLUSIONS
  The Determine rapid HIV antibody test is sensitive, specific, easy to perform and provides a valuable method for HIV testing especially in settings with limited access to laboratory infrastructures and trained laboratory staff.
authors.affiliation: Department of Physiology, University of Natal Durban.
authors.forename: R
authors.initials: R
authors.lastname: Phili
authors.forename: E
authors.initials: E
authors.lastname: Vardas
mesh_terms: D015492:AIDS Serodiagnosis / Q000191:economics / Q000379:methods* / Q000592:standards; D000704:Analysis of Variance; D016009:Chi-Square Distribution; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000379:methods* / Q000592:standards; D005260:Female; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D015528:HIV Seroprevalence; D006801:Humans; D008403:Mass Screening / Q000191:economics / Q000379:methods* / Q000592:standards; D010865:Pilot Projects; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D011320:Primary Health Care; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013019:South Africa / Q000453:epidemiology; D013997:Time Factors