Leaderless mRNAs bind 70S ribosomes more strongly than 30S ribosomal subunits in Escherichia coli.
By primer extension inhibition assays, 70S ribosomes bound with higher affinity, or stability, than did 30S subunits to leaderless mRNAs containing AUG or GUG start codons. Addition of translation initiation factors affected ribosome binding to leaderless mRNAs. Our results suggest that translation of leaderless mRNAs might initiate through a pathway involving 70S ribosomes or 30S subunits lacking IF3.
O'Donnell
Sean M
SM
Department of Microbiology, Miami University, Oxford, Ohio 45056, USA.
Janssen
Gary R
GR
D018387:Codon, Initiator; D004926:Escherichia coli / Q000235:genetics*; D039662:Prokaryotic Initiation Factors; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*
D018387:Codon, Initiator; D039662:Prokaryotic Initiation Factors; D012333:RNA, Messenger