A neoantigenic determinant in the D-dimer fragment of fibrin.
Monoclonal antibody (mAb) III-3b binds D-dimer with K(d)=1.4 x 10(-10) M without cross-reaction with fibrin(ogen). The epitope for this mAb is in Bbeta134-190, presumably in Bbeta155-160. The latter site is buried in the coiled coil structure of fibrin(ogen) but it is exposed as a neoantigenic determinant in D-dimer upon plasmic lysis of fibrin. mAb III-3b may be used as a tool for immunodiagnostic quantification of D-dimer in blood plasma.
Lugovskoy
E V
EV
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, 9 Leontovicha street, Kiev 01601, Ukraine.
Kolesnikova
I N
IN
Gritsenko
P G
PG
Zolotareva
E N
EN
Gaffney
P
P
Nieuwenhuizen
W
W
Komisarenko
S V
SV
D000911:Antibodies, Monoclonal / Q000276:immunology; D000915:Antibody Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D000939:Epitopes; D005337:Fibrin / Q000276:immunology*; D005338:Fibrin Fibrinogen Degradation Products / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005338:Fibrin Fibrinogen Degradation Products; C036309:fibrin fragment D; D005337:Fibrin