title: Hepatocyte nuclear factor-4 alpha mediates the stimulatory effect of peroxisome proliferator-activated receptor gamma co-activator-1 alpha (PGC-1 alpha) on glucose-6-phosphatase catalytic subunit gene transcription in H4IIE cells.
abstract: It has recently been shown that adenoviral-mediated expression of peroxisome proliferator-activated receptor gamma co-activator-1 alpha (PGC-1 alpha) in hepatocytes stimulates glucose-6-phosphatase catalytic subunit (G6Pase) gene expression. A combination of fusion gene, gel retardation and chromatin immunoprecipitation assays revealed that, in H4IIE cells, PGC-1 alpha mediates this stimulation through an evolutionarily conserved region of the G6Pase promoter that binds hepatocyte nuclear factor-4 alpha.
authors.affiliation: Department of Molecular Physiology and Biophysics, Vanderbilt University Medical School, Nashville, Tennessee 37232, USA.
authors.forename: Jared N
authors.initials: JN
authors.lastname: Boustead
authors.forename: Beth T
authors.initials: BT
authors.lastname: Stadelmaier
authors.forename: Angela M
authors.initials: AM
authors.lastname: Eeds
authors.forename: Peter O
authors.initials: PO
authors.lastname: Wiebe
authors.forename: Christina A
authors.initials: CA
authors.lastname: Svitek
authors.forename: James K
authors.initials: JK
authors.lastname: Oeser
authors.forename: Richard M
authors.initials: RM
authors.lastname: O'Brien
mesh_terms: D000818:Animals; D001483:Base Sequence; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D001665:Binding Sites; D020134:Catalytic Domain; D017931:DNA Primers; D004268:DNA-Binding Proteins; D015971:Gene Expression Regulation, Enzymologic; D005952:Glucose-6-Phosphatase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051557:Hepatocyte Nuclear Factor 4; D051379:Mice; D010750:Phosphoproteins / Q000378:metabolism / Q000502:physiology*; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D017384:Sequence Deletion; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D017931:DNA Primers; D004268:DNA-Binding Proteins; D051557:Hepatocyte Nuclear Factor 4; D010750:Phosphoproteins; D018160:Receptors, Cytoplasmic and Nuclear; C501970:Tcfl4 protein, mouse; D014157:Transcription Factors; C111264:peroxisome-proliferator-activated receptor-gamma coactivator-1; D005952:Glucose-6-Phosphatase