title: Genomic instability of prawn white spot bacilliform virus (WSBV) and its association to virus virulence.
abstract: Prawn White Spot Bacilliform Virus (WSBV) is a major pathogen that causes prawn diseases. In this study, we examined the sequence of WSBV genome DNA in the shrimp Penaeus japonicus, P. vannamei, P. Monodon, P. chinensis and Metapenaeus ensis through successive PCR amplification of the DNA fragments in the whole WSBV genome. We found a sequence deletion hotspot in the WSBV genome that is 305107 bp in length. The sizes of the deleted fragments were 4.6, 4.8 or 8.1 kbp depending on the species of prawn. Since the mortality of shrimp infected by the intact WSBV was always significantly higher than that of shrimp infected by DNA fragment-deleted WSBV, we suggest that this deletion be somehow linked to the virulence of the virus itself. This result may lead to the discovery of the molecular mechanism of the pathogenicity of WSBV in shrimps.
authors.affiliation: School of Life Science, Xiamen University, Xiamen 361005, PR China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Lan
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Xun
authors.initials: X
authors.lastname: Xu
mesh_terms: D000818:Animals; D001483:Base Sequence; D004267:DNA Viruses / Q000235:genetics / Q000472:pathogenicity*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D017353:Gene Deletion; D016679:Genome, Viral; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000821:virology*; D017422:Sequence Analysis, DNA; D014774:Virulence
chemical_list: D004279:DNA, Viral