Neuronal NO synthase and its inhibitor PIN are present and influenced by glucose in the human beta-cell line CM and in rat INS-1 cells.
Nitric oxide (NO) is synthesised by different nitric oxide synthases (NOS) from L-arginine and acts as a signal transducer in a variety of cells. The neuronal isoenzyme of NOS (nNOS) was recently found in rodent beta-cells and beta-cell lines. We provide evidence that nNOS is also present in the human beta-cell line CM and that the specific inhibitor of nNOS PIN is expressed in CM and INS-1 cells. Furthermore, we investigated the influence of glucose on the activity of nNOS and the expression of PIN and are able to show that both are increased by glucose stimulation in the beta-cell lines but not in the mouse fibroblastic cell line LTK. This indicates that nNOS and PIN play a role in the specific function of beta-cells, not only in rodents but also in humans.
Dorff
Gernot
G
Department of Biochemistry, Hannover Medical School, Germany.
Meyer
Gustav
G
Krone
Dorothee
D
Pozzilli
Paolo
P
Zühlke
Hartmut
H
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D029721:Drosophila Proteins; D004398:Dyneins; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007515:Islets of Langerhans / Q000201:enzymology* / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
D002352:Carrier Proteins; D029721:Drosophila Proteins; D004247:DNA; C496320:NOS1 protein, human; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat; D004398:Dyneins; D005947:Glucose