The canine minute virus (minute virus of canines) is a distinct parvovirus that is most similar to bovine parvovirus.
We characterized the genome and proteins of the canine minute virus (the minute virus of canines (MVC)). The genome sequence showed MVC to be an autonomous parvovirus encoding a large nonstructural protein 1 gene, a smaller nonstructural protein, and overlapping VP1 and VP2 protein genes. The virus was most closely related to bovine parvovirus (BPV), with which it was 43% identical at the DNA sequence level, while the NS1 and VP1 proteins were 33.6 and 41.4% identical to those of BPV, respectively. Spliced messages of the NS1 gene transcripts were detected by RT-PCR. VP1 and VP2 proteins were detected in purified capsids, as were modified versions of each protein, and VP3 was also found in full capsids.
Schwartz
Daniel
D
James A. Baker Institute and Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Green
Bryan
B
Carmichael
Leland E
LE
Parrish
Colin R
CR
D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000235:genetics*; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D004285:Dogs / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017992:Parvovirus / Q000145:classification* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins / Q000235:genetics*
D036022:Capsid Proteins; C106202:NS1 protein, parvovirus; D017361:Viral Nonstructural Proteins