Short-term and long-term modulation of gene expression by antisense therapeutics.
To achieve effective modulation of gene expression by antisense oligonucleotides, novel oligonucleotide chemistries that do not promote RNase H degradation of target RNA are needed. In addition to short-term oligonucleotide effects, long-term gene regulation can be accomplished by intracellularly expressed antisense RNAs delivered by viral vectors.
Sazani
Peter
P
Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina, CB 7295, Chapel Hill, NC 27599-7295, USA.
Vacek
Marla M
MM
Kole
Ryszard
R
D005786:Gene Expression Regulation / Q000187:drug effects*; D015316:Genetic Therapy / Q000379:methods; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000235:genetics* / Q000494:pharmacology; D014176:Protein Biosynthesis; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics; D016372:RNA, Antisense / Q000737:chemistry* / Q000235:genetics* / Q000494:pharmacology; D016914:Ribonuclease H / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D016376:Oligonucleotides, Antisense; D016372:RNA, Antisense; D016914:Ribonuclease H