Activation of myosin V-based motility and F-actin-dependent network formation of endoplasmic reticulum during mitosis.
It is widely believed that microtubule- and F-actin-based transport of cytoplasmic organelles and membrane fusion is down-regulated during mitosis. Here we show that during the transition of Xenopus egg extracts from interphase to metaphase myosin V-driven movement of small globular vesicles along F-actin is strongly inhibited. In contrast, the movement of ER and ER network formation on F-actin is up-regulated in metaphase extracts. Our data demonstrate that myosin V-driven motility of distinct organelles is differently controlled during the cell cycle and suggest an active role of F-actin in partitioning, positioning, and membrane fusion of the ER during cell division.
Wollert
Torsten
T
Institute of Cell Biology and Biosystems Technology, University of Rostock, D-18051 Rostock, Germany.
Weiss
Dieter G
DG
Gerdes
Hans-Hermann
HH
Kuznetsov
Sergei A
SA
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000502:physiology*; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D002458:Cell Fractionation; D022162:Cytoplasmic Vesicles / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D008561:Membrane Fusion / Q000502:physiology; D008938:Mitosis / Q000502:physiology*; D020409:Molecular Motor Proteins / Q000378:metabolism; D024701:Myosin Type V / Q000378:metabolism*; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D015388:Organelles / Q000378:metabolism; D019311:Staurosporine / Q000494:pharmacology; D014982:Xenopus laevis / Q000502:physiology
D000199:Actins; D020409:Molecular Motor Proteins; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D024701:Myosin Type V; D019311:Staurosporine