title: Nitric oxide promotes differentiation of rat white preadipocytes in culture.
abstract: The putative role of nitric oxide (NO) in modulating adipogenesis was investigated in cultured preadipocytes derived from rat white adipose tissue. The NO releasing reagent, hydroxylamine (HA), and nitric oxide synthase (NOS) substrate L-arginine (Arg) had no influence on cell replication. However, both HA and Arg exhibited significant induction on differentiation, as evidenced by increased lipoprotein lipase (LPL) and glycerol-3-phosphate dehydrogenase (GPDH) activities, as well as accelerated triacylglycerol (TG) accumulation. These observations suggested a positive role of NO in modulating adipogenesis. Preadipocytes were found to produce NO, and a approximately 50% increase over basal level was observed on the first 2 days of differentiation. Deprivation of endogenous NOS activity by a non-selective NOS inhibitor, N(G)-monomethyl-L-arginine (NMMA), partially abrogated the differentiation process, implicating a role for endogenous NO to stimulate preadipocyte differentiation. Both NOS isoforms, eNOS and iNOS, were detected in differentiating preadipocytes. Specific iNOS inhibitors (1400W and aminoguanidine) had little influence on NO production and differentiation, suggesting that eNOS rather than iNOS may be the major isoform involved in modulating adipogenesis.
authors.affiliation: Julia McFarlane Diabetes Research Centre, Departments of Medicine, Biochemistry and Molecular Biology, The University of Calgary, Calgary, AB, T2N 4N1, Canada.
authors.forename: Hongyun
authors.initials: H
authors.lastname: Yan
authors.forename: Edith
authors.initials: E
authors.lastname: Aziz
authors.forename: Gillian
authors.initials: G
authors.lastname: Shillabeer
authors.forename: Alex
authors.initials: A
authors.lastname: Wong
authors.forename: Deena
authors.initials: D
authors.lastname: Shanghavi
authors.forename: Abdenaim
authors.initials: A
authors.lastname: Kermouni
authors.forename: Mohammed
authors.initials: M
authors.lastname: Abdel-Hafez
authors.forename: David C W
authors.initials: DC
authors.lastname: Lau
mesh_terms: D017667:Adipocytes / Q000502:physiology*; D000818:Animals; D001120:Arginine / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D019811:Hydroxylamine / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019811:Hydroxylamine; D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase