Hexadecylphosphocholine selectively upregulates expression of intracellular adhesion molecule-1 and class I major histocompatibility complex antigen in human monocytes.
U 937 cells are widely used as a model system to study human monocytes, since they express typical human monocyte markers and properties. Hexadecylphosphocholine (HPC) is the main representative of a class of synthetic phospholipids, the alkylphosphocholines (APCs), and is able to form stable multilamellar vesicles (MLVs = liposomes) to deliver HPC to monocytes/macrophages. Here we report the ability of both micellar and liposomal HPC (L-HPC) to interact with human monocytes and upregulate specific adhesion molecules. Whereas CD14 could neither be induced by HPC nor by L-HPC on U 937 cells, intracellular adhesion molecule-1 and class 1 major histocompatibility complex (MHC-1) antigen were upregulated by both HPC and L-HPC in a dose-dependent manner. These data support and complete previous studies on HPC-induced activation of U 937 cells and provide additional mechanistic information on the initial steps of HPC-mediated recruitment of macrophages and their antitumor activity.
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ineseue@aol.com
D000970:Antineoplastic Agents / Q000494:pharmacology*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D008081:Liposomes; D008823:Micelles; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D020298:U937 Cells; D015854:Up-Regulation
D000970:Antineoplastic Agents; D015395:Histocompatibility Antigens Class I; D018950:Lipopolysaccharide Receptors; D008081:Liposomes; D008823:Micelles; D010767:Phosphorylcholine; D018799:Intercellular Adhesion Molecule-1; C039128:miltefosine