title: Mutagenicity and in vivo toxicity of combined particulate and semivolatile organic fractions of gasoline and diesel engine emissions.
abstract: Exposure to engine emissions is associated with adverse health effects. However, little is known about the relative effects of emissions produced by different operating conditions, fuels, or technologies. Rapid screening techniques are needed to compare the biological effects of emissions with different characteristics. Here, we examined a set of engine emission samples using conventional bioassays. The samples included combined particulate material and semivolatile organic compound fractions of emissions collected from normal- and high-emitter gasoline and diesel vehicles collected at 72 degrees F, and from normal-emitter groups collected at 30 degrees F. The relative potency of the samples was determined by statistical analysis of the dose-response curves. All samples induced bacterial mutagenicity, with a 10-fold range of potency among the samples. Responses to intratracheal instillation in rats indicated generally parallel rankings of the samples by multiple endpoints reflecting cytotoxic, inflammatory, and lung parenchymal changes, allowing selection of a more limited set of parameters for future studies. The parameters selected to assess oxidative stress and macrophage function yielded little useful information. Responses to instillation indicated little difference in potency per unit of combined particulate material and semivolatile organic compound mass between normal-emitter gasoline and diesel vehicles, or between emissions collected at different temperatures. However, equivalent masses of emissions from high-emitter vehicles of both types were more potent than those from normal-emitters. While preliminary in terms of assessing contributions of different emissions to health hazards, the results indicate that a subset of this panel of assays will be useful in providing rapid, cost-effective feedback on the biological impact of modified technology.
authors.affiliation: Lovelace Respiratory Research Institute, 2425 Ridgecrest Dr SE, Albuquerque, New Mexico 87108, USA. jseagrav@lrri.org
authors.forename: JeanClare
authors.initials: J
authors.lastname: Seagrave
authors.forename: Jacob D
authors.initials: JD
authors.lastname: McDonald
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Gigliotti
authors.forename: Kristen J
authors.initials: KJ
authors.lastname: Nikula
authors.forename: Steven K
authors.initials: SK
authors.lastname: Seilkop
authors.forename: Michael
authors.initials: M
authors.lastname: Gurevich
authors.forename: Joe L
authors.initials: JL
authors.lastname: Mauderly
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D005742:Gasoline; D007322:Instillation, Drug; D007442:Intubation, Intratracheal; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007958:Leukocyte Count; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D016676:Macrophages, Alveolar / Q000378:metabolism; D008297:Male; D009152:Mutagenicity Tests; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D011506:Proteins / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012475:Salmonella / Q000187:drug effects* / Q000235:genetics; D001335:Vehicle Emissions / Q000032:analysis / Q000633:toxicity*; D014835:Volatilization
chemical_list: D000393:Air Pollutants; D005742:Gasoline; D011506:Proteins; D001335:Vehicle Emissions; D007770:L-Lactate Dehydrogenase