title: Use of bulk milk for detection of Neospora caninum infection in dairy herds in Thailand.
abstract: The relationship between the level of Neospora caninum antibodies in bulk milk and the seroprevalence in lactating cows was investigated. Bulk milk was also used to estimate the prevalence of N. caninum infection in dairy herds in the northeast and north Thailand. Bulk milk and individual serum from all lactating cows in 11 herds as well as 220 bulk milk samples from nine milk collection centres were analysed for presence of N. caninum antibodies using an iscom ELISA. In the 11 herds the bulk milk absorbances ranged between 0.04 and 0.89 and the seroprevalences varied between 0 and 46%. Five herds had milk absorbances below 0.20, among those were the two herds housing only seronegative lactating cows. In the remaining three herds with such low bulk milk absorbances one or two cows (5-14%) were seropositive. Six of the investigated herds had bulk milk absorbances above 0.20. In the two herds with the highest bulk milk absorbances more than 30% of the cows were seropositive. Using an absorbance of 0.20 to discriminate between negative and positive herds, 102 (46%) of 220 bulk milk samples were judged positive. There was no significant difference in mean bulk milk absorbance between the milk collection centres within each region. However, the proportion of herds with bulk milk absorbances > or =0.50 in the north was statistically (P < 0.01) higher than that in the northeast. It was concluded that bulk milk antibody testing can be used to identify N. caninum-infected herds and that N. caninum is a common infection in dairy herds in Thailand.
authors.affiliation: Department of Ruminant Medicine and Veterinary Epidemiology, Swedish University of Agricultural Sciences, PO Box 7019, SE-75007 Uppsala, Sweden.
authors.forename: Aran
authors.initials: A
authors.lastname: Chanlun
authors.forename: Katarina
authors.initials: K
authors.lastname: Näslund
authors.forename: Suneerat
authors.initials: S
authors.lastname: Aiumlamai
authors.forename: Camilla
authors.initials: C
authors.lastname: Björkman
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D003048:Coccidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007774:Lactation; D008892:Milk / Q000469:parasitology*; D018688:Neospora / Q000302:isolation & purification*; D016036:Seroepidemiologic Studies; D013785:Thailand / Q000453:epidemiology
chemical_list: D000914:Antibodies, Viral