title: Early and late gene changes in MPTP mice model of Parkinson's disease employing cDNA microarray.
abstract: Recently, we reported specific brain gene expression changes in the chronic MPTP model inthe late stage of degeneration, employing cDNA expression array, which indicate a "domino" cascade of events involved in neuronal cell death. In an attempt to elucidate early gene expression profile in the region of the substantia nigra (SN) and the striatum of acute MPTP-treated mice (3-24 h), we elected a restricted number of genes affected by the long-term MPTP treatment, and their expression was examined. Specifically, we detected alterations in the expression of genes implicated in oxidative-stress, inflammatory processes, signal transduction and glutamate toxicity. These pro-toxic genes appear to be compensated by the elevated expression in trophic factors and antioxidant defenses, which are also activated by short exposure to MPTP. The time course of these gene expression changes indicates the importance of investigating the early gene cascade of events occurring prior to late nigrostriatal dopamine neuronal cell death.
authors.affiliation: Eye Topf and U.S. National Parkinson's Foundation Centers of Excellence for Neurodegenerative Diseases, Bruce Rappaport Family Research Institute and Department of Pharmacology, Haifa, Israel.
authors.forename: Silvia
authors.initials: S
authors.lastname: Mandel
authors.forename: Edna
authors.initials: E
authors.lastname: Grünblatt
authors.forename: Gila
authors.initials: G
authors.lastname: Maor
authors.forename: Moussa B H
authors.initials: MB
authors.lastname: Youdim
mesh_terms: D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000503:physiopathology; D018076:DNA, Complementary / Q000235:genetics*; D015259:Dopamine Agents; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D010302:Parkinson Disease, Secondary / Q000139:chemically induced* / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D015259:Dopamine Agents; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D014446:Tyrosine 3-Monooxygenase