title: Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains.
abstract: In the present study, we have analyzed by sodium docecyl sulphate - polyacrilamide gel electrophoresis (SDS-PAGE), immunoblotting and Concanavalin A blotting (Con A blotting) proteins of membrane fractions and soluble fractions obtained from Giardia duodenalis trophozoites of two axenic strains isolated in Brazil from a symptomatic (BTU-11) and an asymptomatic patient (BTU-10), as compared to the reference strain Portland 1. Both Brazilian strains showed a complex and homogeneous electrophoretic pattern of proteins, but some differences could be observed. Several glycoproteins were detected, particularly the proteins of 81, 72, 59 kDa and the protein of 62 kDa in the membrane proteins and cytosol, respectively. Many antigenic components were revealed by anti-Giardia rabbit IgG antibodies in the immunoblotting analysis. Among these components, the membrane protein of 32 kDa and the cytosol protein of 30 kDa could be related to giardin, as previously demonstrated.
authors.affiliation: Departamento de Parasitologia, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, São Paulo, Brasil. sgviana@ibb.unesp.br
authors.forename: Semíramis
authors.initials: S
authors.lastname: Guimarães
authors.forename: Maria Inês T Leme
authors.initials: MI
authors.lastname: Sogayar
authors.forename: Marcello
authors.initials: M
authors.lastname: Franco
mesh_terms: D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005872:Giardia / Q000737:chemistry* / Q000276:immunology / Q000472:pathogenicity; D006790:Host-Parasite Interactions; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D015800:Protozoan Proteins / Q000032:analysis*; D011817:Rabbits
chemical_list: D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D008565:Membrane Proteins; D015800:Protozoan Proteins