White light-mediated DNA strand breaks in lens epithelial cells.
It was hypothesized that white light can induce DNA single-strand breaks/alkali labile sites in lens epithelial cells during the dissection process when lenses are being collected for study. Dissection of lenses in white light significantly increased 'Integrated Intensity' (P=0.0216), an index of DNA single strand breaks/alkali labile sites, using the alkaline microgel electrophoresis assay. In addition, we investigated the DNA damaging effect of visible light in a human lymphocyte model in which cells were exposed to white light, red light or minimal indirect light for 5 min. Results of these experiments confirm our conclusion that visible light induces DNA damage in cells. These findings may be relevant in a genotoxicity study on cells ex vivo.
Singh
Narendra P
NP
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA. narendra@u.washington.edu
Penn
Phillip E
PE
Pendergrass
William R
WR
Wolf
Norman S
NS
D000818:Animals; D020552:Comet Assay; D004249:DNA Damage / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics*; D004848:Epithelium / Q000502:physiology; D006801:Humans; D007908:Lens, Crystalline / Q000502:physiology*; D008027:Light; D008214:Lymphocytes / Q000502:physiology; D051381:Rats
D004277:DNA, Single-Stranded