Structural analysis of the oligosaccharide alditols released from the jelly coat of Rana dalmatina eggs by reductive beta-elimination.
A combination of ion-exchange chromatography and high performance liquid chromatography (HPLC) has been used to separate the reduced oligosaccharides produced by alkaline borohydride degradation of oviducal mucins obtained from the jelly coat of Rana dalmatina. The primary structures of 26 O-glycans were determined by one-dimensional and two-dimensional 1H and 1H13C NMR spectroscopy. As observed for 20 other amphibian species, these carbohydrate chains are highly species-specific. The main typical feature of the species R. dalmatina consists in the presence of the backbone Gal(beta1-3)[Gal(beta1-4)]Gal(beta1-3)GalNAc-ol, previously observed among Ranidae, such as R. temporaria and R. ridibunda. Nevertheless, the nature of carbohydrates present at the periphery of the glycans perfectly differentiates the three species.
Florea
Doina
D
Departamentul de Biochimie, Facultatea de Biologie, Universitatea din Bucuresti, Splaiul Independentei 91-95, 76201 Bucuresti, Romania.
Maes
Emmanuel
E
Haddad
Mohamed
M
Strecker
Gérard
G
D000818:Animals; D001894:Borohydrides / Q000378:metabolism; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009865:Oocytes / Q000378:metabolism*; D011898:Ranidae / Q000378:metabolism*; D013045:Species Specificity; D013402:Sugar Alcohols / Q000737:chemistry* / Q000302:isolation & purification
D001894:Borohydrides; D009077:Mucins; D009844:Oligosaccharides; D013402:Sugar Alcohols