Inhibitory effect of chlorophyllin on the frequency of micronuclei induced by sodium nitrite in mice.
In this report the potency of chlorophyllin (CHL) was evaluated to prevent two types of damage produced by nitrite in mice: the increase of micronucleated polychromatic erythrocytes (MNPE) and the bone marrow toxicity, measured as the index of polychromatic erythrocytes/normochromatic erythrocytes (PE/NE). The study was done in eight groups of male mice. The first three groups were administered orally for 4 days with sodium nitrite (10, 15 and 20 mg/kg), the daily administration with nitrite was followed by an intraperitoneal administration of CHL (4 mg/kg), three more groups were administered with the same amounts of nitrite, a seventh group of mice was treated with distilled water while another was treated with CHL (4 mg/kg). Our study produced two main results: (a) no bone marrow injury was induced by any of the tested chemicals, as indicated with the PE/NE index, and (b) CHL protected (as high as 44%) the MNPE produced in nitrite treated mice.
Diaz-Barriga Arceo
S
S
Laboratorio de Citogenética FESC-UNAM, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México.
Hernández-Ceruelos
A
A
Madrigal-Bujaidar
E
E
Chamorro
G
G
D000284:Administration, Oral; D000818:Animals; D016587:Antimutagenic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002735:Chlorophyllides / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects* / Q000473:pathology; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects / Q000473:pathology; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens / Q000008:administration & dosage / Q000494:pharmacology*; D008517:Phytotherapy; D012977:Sodium Nitrite / Q000008:administration & dosage / Q000494:pharmacology*
D016587:Antimutagenic Agents; D002735:Chlorophyllides; D009153:Mutagens; C007020:chlorophyllin; D012977:Sodium Nitrite