title: A versatile and scalable two-step ion-exchange chromatography process for the purification of recombinant adeno-associated virus serotypes-2 and -5.
abstract: Here we describe the development of a two-step chromatography process based on the use of ion-exchange resins for the purification of recombinant adeno-associated virus (rAAV) serotypes-2 and-5. In vitro and in vivo results demonstrate that this method, which does not require any prepurification step of the cell lysate, can be applied to obtain highly pure rAAV2 and rAAV5 stocks. As such,this procedure can be easily transferred in vector cores and also scaled up, allowing the direct comparison of these two, and potentially other, AAV serotypes in large animal models.
authors.affiliation: INSERM ERM 0105 Laboratoire de Thérapie Génique, CHU Hotel Dieu, Nantes, France.
authors.forename: Nicole
authors.initials: N
authors.lastname: Brument
authors.forename: Robert
authors.initials: R
authors.lastname: Morenweiser
authors.forename: Véronique
authors.initials: V
authors.lastname: Blouin
authors.forename: Estelle
authors.initials: E
authors.lastname: Toublanc
authors.forename: Isabelle
authors.initials: I
authors.lastname: Raimbaud
authors.forename: Yan
authors.initials: Y
authors.lastname: Chérel
authors.forename: Sébastien
authors.initials: S
authors.lastname: Folliot
authors.forename: Florence
authors.initials: F
authors.lastname: Gaden
authors.forename: Pierre
authors.initials: P
authors.lastname: Boulanger
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Kroner-Lux
authors.forename: Philippe
authors.initials: P
authors.lastname: Moullier
authors.forename: Fabienne
authors.initials: F
authors.lastname: Rolling
authors.forename: Anna
authors.initials: A
authors.lastname: Salvetti
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002586:Cesium / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D002852:Chromatography, Ion Exchange / Q000379:methods*; D002853:Chromatography, Liquid; D000229:Dependovirus / Q000235:genetics* / Q000302:isolation & purification*; D005821:Genetic Techniques; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D010957:Plasmids / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014283:Triiodobenzoic Acids / Q000494:pharmacology
chemical_list: D002712:Chlorides; D014283:Triiodobenzoic Acids; D002586:Cesium; C028019:cesium chloride; C044834:iodixanol