title: Mutagenic transgene insertion into a region of high gene density and multiple linkage disruptions on mouse chromosome 11.
abstract: We have characterized a 185-kb contig surrounding a transgene on distal mouse chromosome 11, the insertion of which has caused a recessive phenotype with skeletal malformations. By cDNA selection and sequencing we have found six genes (Lasp1, Rpl23, Mllt6, Pip5k2b, Psmb3, Zfp144), one truncated gene (Mel13), and one pseudogene (Rps15-ps) within this region. The murine Mllt6 gene is new, it was identified by its high homology (90% identity) with the human homologue MLLT6. Psmb3 and Pip5k2b had not yet been assigned to mouse chromosomes. A comparison with the corresponding region on human chromosome 17q12 revealed several small-scale rearrangements during evolutionary divergence within this cluster of densely packed genes.
authors.affiliation: Institute of Molecular Biology, Salzburg, Austria.
authors.forename: N
authors.initials: N
authors.lastname: Kleiter
authors.forename: I
authors.initials: I
authors.lastname: Artner
authors.forename: N
authors.initials: N
authors.lastname: Gmachl
authors.forename: N
authors.initials: N
authors.lastname: Ghaffari-Tabrizi
authors.forename: K
authors.initials: K
authors.lastname: Kratochwil
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001483:Base Sequence; D033481:Chromosomes, Mammalian / Q000235:genetics*; D003094:Collagen / Q000235:genetics; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D003598:Cytoskeletal Proteins; D004251:DNA Transposable Elements; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D019143:Evolution, Molecular; D015321:Gene Rearrangement; D008040:Genetic Linkage; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D007506:Iron-Sulfur Proteins; D060588:LIM Domain Proteins; D007763:Lac Operon; D051379:Mice; D008822:Mice, Transgenic; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics; D020161:Physical Chromosome Mapping; D011506:Proteins / Q000235:genetics; D011544:Pseudogenes; D012269:Ribosomal Proteins / Q000235:genetics; D013045:Species Specificity
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C098536:Cisd3 protein, mouse; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D003598:Cytoskeletal Proteins; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D007506:Iron-Sulfur Proteins; C096353:LASP1 protein, human; D060588:LIM Domain Proteins; C490692:Lasp1 protein, mouse; C096106:MLLT6 protein, human; D024101:Mitochondrial Proteins; C486273:Mllt6 protein, mouse; D009363:Neoplasm Proteins; D011506:Proteins; D012269:Ribosomal Proteins; C036004:ribosomal protein S15; D003094:Collagen