title: Self-assembled ternary complex of cationic dendrimer, cucurbituril, and DNA: noncovalent strategy in developing a gene delivery carrier.
abstract: A ternary complex of PPI-DAB dendrimer [(1,4-diaminobutane); Gen = N; dendri-poly(propyleneimine); -[NHC(=O)CH(2)NH(2)(+)(CH(2))(4)NH(3)(+)](z)()], DNA, and cucurbituril (CB) was evaluated as an example of a totally self-assembled gene delivery carrier. The complex was formed in a noncovalent way in which DNA interacts with PPI-DAB electrostatistically and CB with PPI-DAB through multiple noncovalent interactions. Dynamic light scattering data indicated that the diameter and size distributions of the complexes were dependent upon the sequence of mixing of each component with unimodal distribution ranging from 150.8 to 210.2 nm under favorable conditions. Fluorescence studies showed the quantitative binding of CB to PPI-DAB after ternary complex formation. The complex was able to transfect mammalian cells with high efficiency and the cytotoxicity of the PPI-DAB/CB complex was relatively low.
authors.affiliation: School of Chemistry & Molecular Engineering, Seoul National University, Seoul 151-742, Korea.
authors.forename: Yong-Beom
authors.initials: YB
authors.lastname: Lim
authors.forename: Taeil
authors.initials: T
authors.lastname: Kim
authors.forename: Jae Wook
authors.initials: JW
authors.lastname: Lee
authors.forename: Seon-Mi
authors.initials: SM
authors.lastname: Kim
authors.forename: Hee-Joon
authors.initials: HJ
authors.lastname: Kim
authors.forename: Kimoon
authors.initials: K
authors.lastname: Kim
authors.forename: Jong-Sang
authors.initials: JS
authors.lastname: Park
mesh_terms: D000818:Animals; D001952:Bridged-Ring Compounds / Q000009:adverse effects / Q000737:chemistry*; D002412:Cations / Q000009:adverse effects / Q000737:chemistry*; D002522:Chlorocebus aethiops; D004247:DNA / Q000009:adverse effects / Q000737:chemistry*; D004337:Drug Carriers / Q000009:adverse effects / Q000737:chemistry*; D007093:Imidazoles / Q000009:adverse effects / Q000737:chemistry*; D008027:Light; D011108:Polymers / Q000009:adverse effects / Q000737:chemistry*; D011700:Putrescine / Q000009:adverse effects / Q000737:chemistry*; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D055672:Static Electricity; D014162:Transfection / Q000379:methods*; D014709:Vero Cells; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils
chemical_list: D001952:Bridged-Ring Compounds; D002412:Cations; D004247:DNA; D004337:Drug Carriers; D007093:Imidazoles; D011108:Polymers; D011700:Putrescine; C454805:cucurbit(6)uril; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils