Dolichol levels in younger and older rat hearts heterotopically transplanted in younger recipients.
Dolichol (D) levels increase dramatically in older tissue. An understanding of the exchangeability of D between tissues may be essential in order to understand the mechanism of the abnormal accumulation associated with aging. The question was investigated by the use of organ transplantation. D-poor hearts donated by 3-mon-old and D-rich by 22-mon-old male Lewis rats were transplanted heterotopically in 3-mon-old syngenic recipients, whose peripheral tissues and liver were poor in D. Native and transplanted hearts were taken 7 and 21 d after surgery. Native hearts of 3-mon- and 22-mon-old male Lewis rats served as control. D concentration and quantity were higher in older than in younger native hearts as expected. In the transplanted hearts, the quantity of D was unchanged, irrespective of the age of the donor and of the time of transplantation, whereas D concentration increased because of the remarkable disuse atrophy. No changes in D were observed in recipients' tissues. It is concluded that dolichol is not redistributed via circulation from the transplanted heart to the tissues and liver of the younger recipient.
Cavallini
Gabriella
G
Dipartimento di Patologia sperimentale, Biotecnologie mediche, Infettivologia e Epidemiologia, University of Pisa, Italy.
Parentini
Ilaeia
I
Di Stefano
Rossella
R
Maccheroni
Marco
M
Masini
Matilde
M
Pollera
Maria
M
Gori
Zina
Z
Mosca
Franco
F
Bergamini
Ettore
E
D000375:Aging / Q000502:physiology*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004286:Dolichols / Q000032:analysis*; D016027:Heart Transplantation; D008297:Male; D009206:Myocardium / Q000737:chemistry* / Q000166:cytology; D009929:Organ Size; D051381:Rats; D011919:Rats, Inbred Strains; D017207:Rats, Sprague-Dawley; D014019:Tissue Donors
D004286:Dolichols