Phosphorylation of Bruton's tyrosine kinase by c-Abl.
Bruton's tyrosine kinase (Btk) is necessary for B-lymphocyte development. Mutation in the gene coding for Btk causes X-linked agammaglobulinemia (XLA) in humans. Similar to Btk, c-Abl is a tyrosine kinase shuttling between the cytoplasm and the nucleus where it is involved in different functions depending on the localization. In this report we describe for the first time that c-Abl and Btk physically interact and that c-Abl can phosphorylate tyrosine 223 in the SH3 domain of Btk. Interestingly, the Btk sequence matched a v-Abl substrate [correction] identified from a randomized peptide library and was also highly related to a number of previously found c-Abl substrates.
Bäckesjö
Carl Magnus
CM
Clinical Research Center, Karolinska Institutet, Huddinge University Hospital, Huddinge, Sweden. Magnus.Backesjo@crc.ki.se
Vargas
Leonardo
L
Superti-Furga
Giulio
G
Smith
C I
CI
D000077329:Agammaglobulinaemia Tyrosine Kinase; D020816:Amino Acid Motifs; D000818:Animals; D002460:Cell Line; D006801:Humans; D007150:Immunohistochemistry; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D016315:Proto-Oncogene Proteins c-abl / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D014443:Tyrosine / Q000378:metabolism; D018909:src Homology Domains
D011993:Recombinant Fusion Proteins; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases; D000077329:Agammaglobulinaemia Tyrosine Kinase; C000625949:BTK protein, human; D016315:Proto-Oncogene Proteins c-abl