Androgen induces p130 mRNA expression in the neonatal rat hypothalamus.
Our previous research using cDNA microarray analysis demonstrated that female rats displayed a higher p130 mRNA level than males in the hypothalamus at postnatal day (PN) 5. In the present study, it was shown that at PN3 males had a significantly elevated mRNA level over females, whereas at PN7 females displayed a higher expression level using a real-time reverse transcription-polymerase chain reaction. In situ hybridization analysis indicated relatively strong p130 mRNA signals in the ventromedial nucleus and the arcuate nucleus in the neonatal hypothalamus. Subcutaneous injection of 5alpha-dihydrotestosterone as well as testosterone propionate to PN2 neonatal rats significantly increased p130 gene expression at PN3, whereas estradiol benzoate did not have a significant effect. These results suggest that expression of the p130 gene in the neonatal rat hypothalamus is responsive to androgens and may be involved in sexual differentiation of the brain.
Yonehara
Keisuke
K
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo, Tokyo 113-8657, Japan.
Suzuki
Masatoshi
M
Yamanouchi
Keitaro
K
Nishihara
Masugi
M
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001798:Blood Proteins / Q000096:biosynthesis* / Q000187:drug effects* / Q000235:genetics; D013196:Dihydrotestosterone / Q000494:pharmacology; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007031:Hypothalamus / Q000187:drug effects / Q000502:physiology; D017403:In Situ Hybridization; D008297:Male; D016133:Polymerase Chain Reaction; D011506:Proteins; D012333:RNA, Messenger / Q000187:drug effects; D051381:Rats; D050717:Retinoblastoma-Like Protein p130; D012737:Sex Factors; D013739:Testosterone / Q000494:pharmacology
D000728:Androgens; D001798:Blood Proteins; D011506:Proteins; D012333:RNA, Messenger; C493839:Rbl2 protein, rat; D050717:Retinoblastoma-Like Protein p130; D013196:Dihydrotestosterone; C074283:estradiol 3-benzoate; D013739:Testosterone; D004958:Estradiol