[DNA chip technology and its application in toxicology [correction of toxiology]].
DNA chip is a new molecular biology technology rapidly developed in recent years. It fixes thousands of oligonucleotide on a silicon chip of 1 cm2, hybridizes the measuring materials marked with fluorescein or isotope with probe in DNA chip, and gets the fluorescent signal of hybridized probe through the scan of confocalmicroscope. This technology has been extensively used in HGP, DNA sequencing, transcript analysis, gene diagnosis etc. Toxicology is an important branch of life science. DNA chip can be extensively used in the toxicological [correction of toxiological] field. This paper reviewed the DNA chip technology in mechanism, manufacturing method, signal check, advantages and application prospect in toxicology.
Li
Shu-guang
SG
Institute of Space Medico-Engineering, Beijing, China.
Liang
Hong
H
D000337:Aerospace Medicine / Q000295:instrumentation / Q000379:methods; D001709:Biotechnology; D004247:DNA; D008967:Molecular Biology / Q000295:instrumentation / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis; D014116:Toxicology / Q000295:instrumentation / Q000379:methods*
D004247:DNA