title: Enumeration of Legionella CFU by colony hybridization using specific DNA probes.
abstract: Oligonucleotide probes and colony hybridization (CH) were applied to enumerate organisms of the genus Legionella in cooling tower water. The CH counts indicated almost the same results as CFU counts in cultivated samples derived from the water. It was concluded that it is possible to substitute the CH procedure for the conventional one.
authors.affiliation: Technology and Development Laboratory, Kurita Water Industries Ltd., Kanagawa 243-0124, Japan. wakako.Satoh@kurita.co.jp
authors.forename: Wakako
authors.initials: W
authors.lastname: Satoh
authors.forename: Mayumi
authors.initials: M
authors.lastname: Nakata
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ezaki
authors.forename: Keiichi
authors.initials: K
authors.lastname: Hiramatsu
mesh_terms: D015169:Colony Count, Microbial / Q000379:methods*; D007875:Legionella / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D015345:Oligonucleotide Probes