title: Expression, crystallization and preliminary X-ray studies of the recombinant PTB domain of human dok-5 protein.
abstract: The human dok-5 PTB domain fusion protein has been overexpressed in Escherichia coli and crystallized in a form suitable for X-ray crystallographic study. Crystals were obtained by the vapour-diffusion method. The crystal has unit-cell parameters a = b = 75.9, c = 108.0 A, alpha = beta = 90, gamma = 120 degrees and belongs to space group P3(2)21. Diffraction data were collected to 2.8 A resolution in-house. Furthermore, a selenomethionine (SeMet) derivative of dok-5 PTB domain fusion protein was overexpressed using the same expression system and was purified in a reductive environment. The derivative crystals were obtained under similar conditions. Subsequently, three different wavelength data sets were collected to 2.3 A resolution from the derivative crystal at the Advanced Photon Source, Argonne National Laboratory.
authors.affiliation: MOE Laboratory of Protein Sciences and Laboratory of Structural Biology, Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Shi
authors.forename: Weihong
authors.initials: W
authors.lastname: Zhou
authors.forename: Kaifu
authors.initials: K
authors.lastname: Tang
authors.forename: Yu
authors.initials: Y
authors.lastname: Gao
authors.forename: Jian
authors.initials: J
authors.lastname: Jin
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Xiaozhong
authors.initials: X
authors.lastname: Peng
authors.forename: Mark
authors.initials: M
authors.lastname: Bartlam
authors.forename: Boqin
authors.initials: B
authors.lastname: Qiang
authors.forename: Jiangang
authors.initials: J
authors.lastname: Yuan
authors.forename: Zihe
authors.initials: Z
authors.lastname: Rao
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D010750:Phosphoproteins; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D017931:DNA Primers; C432912:DOK5 protein, human; D010750:Phosphoproteins; D011994:Recombinant Proteins