title: Infection of immortalized human epithelial cell line Hacat with high-concentration Epstein-Barr virus.
abstract: OBJECTIVE
  To study the mechanism by which Epstein-Barr (EB) virus infects human epithelial cells.
  
  
  METHODS
  Large-scale culture of marmoset lymphocytes B958 was carried out to extract and condense EB virus therein. Titrated by lymphocytes from fetal umbilical blood, the EB virus of high concentration was used to infect immortalized human epithelial cell line Hacat, followed by genomic DNA extraction from the Hacat cells and amplification of the special DNA sequence Bam HIw fragments of EBV genomic DNA by PCR. Southern blotting and in situ hybridization were employed to confirm the result of PCR.
  
  
  RESULTS
  The results of PCR, Southern blotting and in situ hybridization all indicated that high-concentration EBV could infect Hacat cell line, but the rate of infection was rather low.
  
  
  CONCLUSIONS
  There is an unknown pathway of infection for EBV entry into the epithelial cells other than the mediation by CR2 or pIgR.
authors.affiliation: Institute of Cancer Research, First Military Medical University, Guangzhou 510515, China.
authors.forename: Pei-Zhou
authors.initials: PZ
authors.lastname: Jiang
authors.forename: Xin-Ming
authors.initials: XM
authors.lastname: Shen
authors.forename: Hua
authors.initials: H
authors.lastname: Huang
authors.forename: Kai-Tai
authors.initials: KT
authors.lastname: Yao
mesh_terms: D002460:Cell Line; D004848:Epithelium / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D016133:Polymerase Chain Reaction; D017464:Receptors, Complement 3d / Q000502:physiology
chemical_list: D017464:Receptors, Complement 3d