Effects of exercise on canine skeletal muscle proteolysis: an investigation of the ubiquitin-proteasome pathway and other metabolic markers.
The effects of long-term athletic training are associated with excessive skeletal muscle turnover attributable to increased rates of myofibrillar protein synthesis and proteolysis, which are mechanisms poorly understood in the athletic dog. A physiologic field study using 44 English pointers and Labrador retrievers that had been purposely bred for bird hunting and retrieving was conducted to examine changes in the ubiquitin-proteasome (UP) pathway, which has been implicated in exercise-induced proteolysis. Muscle biopsy samples were collected from all dogs in September (preseason, pretraining) and February (peak season, peak activity). Western blot analysis was used to assess changes in expression of various components of the UP pathway in the biopsy samples. Citrate synthase and glycogen levels were also measured in a subset of these samples. Results across the population indicated pronounced up-regulation of ubiquitinated conjugates and the p31 regulatory capping subunit during the peak hunting period compared with the preseason period. In contrast, the catalytic core of the proteasome (beta-subunits) showed no apparent up-regulation in response to increased physical activity. Increased physical activity during the hunting season was associated with increased muscle glycogen levels and citrate synthase activity in these dogs. Overall, up-regulation of specific components of the UP pathway was an indication that it plays a role in the proteolytic process associated with skeletal muscle turnover during long-term athletic training, as previously believed.
Wakshlag
Joseph J
JJ
Cornell University, College of Veterinary Medicine, Department of Clinical Sciences, Ithaca, NY 14853, USA.
Kallfelz
Francis A
FA
Barr
Stephen C
SC
Ordway
George
G
Haley
Nicolas J
NJ
Flaherty
Colleen E
CE
Kelley
Russell L
RL
Altom
Eric K
EK
Lepine
Allan J
AJ
Davenport
Gary M
GM
D000818:Animals; D015153:Blotting, Western; D001835:Body Weight; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004285:Dogs / Q000378:metabolism / Q000502:physiology*; D005260:Female; D006003:Glycogen / Q000378:metabolism; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D025801:Ubiquitin / Q000378:metabolism*; D015854:Up-Regulation
D025801:Ubiquitin; D006003:Glycogen; D002950:Citrate (si)-Synthase; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease