Incomplete block of B cell development and immunoglobulin production in mice carrying the muMT mutation on the BALB/c background.
The expression of the preB cell receptor (preBCR), composed of the mu chain, surrogate light chains and the Igalpha /Igbeta signal transduction unit, permits further differentiation of Bcell precursors. C57BL/6 mice homozygous for an inactivating mutation of the membrane exon of the mu chain gene (C57BL/6muMT/muMT)) cannot form a preBCR and are, consequently, devoid of mature B lymphocytes. Here we present evidence that the block of B cell development by the muMT mutation is incomplete in BALB/c mice. Unlike C57BL/6muMT/muMT), BALB/cmuMT/muMT) mice generate small numbers of mature B cells, accumulate plasma cells and produce high levels of all immunoglobulin isotypes, except IgM. The observed phenomenon seems to be controlled by a single genetic locus that is not linked to IgH.
Hasan
Milena
M
Department of Histology and Embryology, Medical Faculty, University of Rijeka, Rijeka, Croatia.
Polic
Bojan
B
Bralic
Marina
M
Jonjic
Stipan
S
Rajewsky
Klaus
K
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D002454:Cell Differentiation; D005362:Ficoll / Q000031:analogs & derivatives* / Q000276:immunology; D018507:Gene Expression Regulation, Developmental; D005803:Genes, Immunoglobulin; D007114:Immunization; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis / Q000235:genetics; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000235:genetics; D007148:Immunoglobulin mu-Chains / Q000235:genetics*; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000235:genetics*; D008070:Lipopolysaccharides / Q000276:immunology; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology; D038041:Lymphopoiesis / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009154:Mutation; D014301:Trinitrobenzenes / Q000276:immunology
D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains; D007136:Immunoglobulins; D008070:Lipopolysaccharides; C020529:TNP-ficoll; D014301:Trinitrobenzenes; C048761:trinitrophenyl-lipopolysaccharide; D005362:Ficoll