Immobilized lipase-catalysed production of alkyl esters of restaurant grease as biodiesel.
Simple alkyl ester derivatives of restaurant grease were prepared using immobilized lipases as biocatalysts. The lipases studied included those of Thermomyces lanuginosa and Candida antarctica supported on granulated silica (gran- T.l. and gran- C.a., respectively), C. antarctica supported on a macroporous acrylic resin (SP435) and Pseudomonas cepacia immobilized within a phyllosilicate sol-gel matrix (IM PS-30). All alcoholysis reactions were carried out in solvent-free media employing a one-step addition of the alcohol to the reaction system. Of the lipases studied, IM PS-30 was found to be the most effective in catalysing the methanolysis and ethanolysis of grease. The processes catalysed by gran- T.l. and gran- C.a. lipases gave poor conversions to esters, and the SP435-catalysed reactions gave intermediate yields of ethyl and methyl esters. Water activity (a(w)) was an important factor in the methanolysis reactions; reaction media with a(w)<0.5 resulted in the highest conversions to methyl esters. Molecular sieves also improved methyl ester yields by as much as 20% in transesterification reactions catalysed by IM PS-30. The immobilized lipases also were evaluated for their ability to produce alkyl esters of grease with several additional normal and branched-chain alcohols.
Hsu
An-Fei
AF
Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA. ahsu@arserrc.gov
Jones
Kerby
K
Foglia
Thomas A
TA
Marmer
William N
WN
D001203:Ascomycota / Q000201:enzymology; D001673:Biodegradation, Environmental; D016956:Burkholderia cepacia / Q000201:enzymology; D002175:Candida / Q000201:enzymology; D002384:Catalysis; D004041:Dietary Fats; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D004951:Esterification; D004952:Esters; D000431:Ethanol / Q000737:chemistry; D005742:Gasoline; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D008567:Membranes, Artificial; D010938:Plant Oils / Q000737:chemistry*; D012147:Restaurants; D013045:Species Specificity; D014865:Waste Disposal, Fluid / Q000379:methods
D004041:Dietary Fats; D004800:Enzymes, Immobilized; D004952:Esters; D005742:Gasoline; D008567:Membranes, Artificial; D010938:Plant Oils; D000431:Ethanol; D008049:Lipase