title: A novel high-cell-density protein expression system based on Ralstonia eutropha.
abstract: We describe the development of a novel protein expression system based on the industrial fermentation organism Ralstonia eutropha (formerly known as Alcaligenes eutrophus) NCIMB 40124. This new system overcomes some of the shortcomings of traditional Escherichia coli-based protein expression systems, particularly the propensity of such systems to form inclusion bodies during high-level expression. Using a proteomics approach, we identified promoters that can be induced by simple process parameters or medium compositions in high-density cell culture or shake flasks, respectively. By combining newly developed molecular biological tools with a high-cell-density fermentation process, we were able to produce high levels (>1 g/liter) of soluble, active organophosphohydrolase, a model enzyme prone to inclusion body formation in E. coli.
authors.affiliation: Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA.
authors.forename: Sriram
authors.initials: S
authors.lastname: Srinivasan
authors.forename: Gavin C
authors.initials: GC
authors.lastname: Barnard
authors.forename: Tillman U
authors.initials: TU
authors.lastname: Gerngross
mesh_terms: D020118:Cupriavidus necator / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D011994:Recombinant Proteins