Effect of angelica on the expressional changes of cytokines in endothelial cells induced by hyperlipidemic serum.
The aim of this article was to examine the protective effect of Chinese traditional medicine angelica on human umbilical vein endothelial cells (HUVECs, ECV304) from injury induced by hyperlipidemic serum (HLS) and to study the underlying mechanism. Spectrophotometer and immunocytochemical methods were used to detect the content of nitric oxide (NO) in suspension and expression of intercellular adhesion molecule-1 (ICAM-1), transforming growth factor beta1 (TGFbeta1), basic fibroblast growth factor (bFGF) on the cell surface, respectively. After incubated with 50 microl/ml HLS for 24 hours, expression of ICAM-1 and bFGF in ECs was significantly increased, while expression of TGFbeta1 and the release of NO from ECs were significantly decreased. All these effect of HLS on ECs can be reversed by angelica significantly. The above effect of angelica may be related to its anti-atherosclerotic action. Our findings provided experimental basement for the clinical application of angelica to prevent the development of atherosclerosis.
Jing-Ping
Ou-Yang
OY
Department of Pathophysiology, Medical College of Wuhan University, Wuhan, 430071, P.R. China. hybs@public.wh.hb.cn
Baohua
Wang
W
Yongming
Liu
L
Lei
Wei
W
Jingwei
Yang
Y
D029969:Angelica; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006801:Humans; D006949:Hyperlipidemias / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D011817:Rabbits; D016212:Transforming Growth Factor beta / Q000378:metabolism; D053773:Transforming Growth Factor beta1
D000975:Antioxidants; D016207:Cytokines; D010936:Plant Extracts; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D016222:Fibroblast Growth Factor 2; D018799:Intercellular Adhesion Molecule-1; D009569:Nitric Oxide