title: Protective effect of ginkgo biloba extract on endothelial cell against damage induced by oxidative stress.
abstract: The viability of bovine aortic endothelial cells (BAECs) treated with 0.1 m H O was decreased by 39.8%, and 100 mg/l EGb761 increased the viability by 20.6%. Exposure BAECs to H O for 6 min resulted in a significant elevation in the intracellular free Ca. Pretreatment of BAECs with 10 mg/l and 100 mg/l EGb761 for 10 min showed a decrease in the intracellular free Ca, 4.5% and 20.6%, respectively. The apoptotic rate of BAECs measured by propidium iodide (PI) staining was (38.1 +/- 2%) after 18 h of treatment with H O. Pretreatment of BAECs with 100 mg/l EGb761 for 1 h reduced the apoptotic rate to 27 +/- 1%. In addition, there were about 5-7% of cells stained positive measured by TUNEL assay. When BAECs were exposed to 0.1 m H O for 18 h, the number of TUNEL-positive cells increased to 37-44%. When 10 mg/l EGb761 and 100 mg/l EGb761 were used, the TUNEL-positive cells decreased to 26.5 +/- 3.1% and 17.5 +/- 1.7%, respectively. Furthermore, EGb761 also inhibited caspase-3 activity induced by H O. It is concluded that EGb761 has protective effect on bovine vascular endothelial cells against damage induced by H O. Further studies are needed to clarify the mechanisms of action of EGb761.
authors.affiliation: Instiute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors.forename: De Cheng
authors.initials: DC
authors.lastname: Ren
authors.forename: Guan-Hua
authors.initials: GH
authors.lastname: Du
authors.forename: Jun Tian
authors.initials: JT
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D020441:Ginkgo biloba; D006861:Hydrogen Peroxide / Q000009:adverse effects* / Q000037:antagonists & inhibitors; D020287:In Situ Nick-End Labeling / Q000379:methods; D018384:Oxidative Stress; D010936:Plant Extracts / Q000627:therapeutic use*; D000096983:Ginkgo Extract
chemical_list: D002118:Calcium; D053148:Caspase 3; D020169:Caspases; D006861:Hydrogen Peroxide; D010936:Plant Extracts; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract