title: New serological assay for detection of putative Helicobacter pylori virulence factors.
abstract: We evaluated a new immunoblot assay (Helicoblot 2.1) for Helicobacter pylori infection and CagA and VacA status by using serum samples from 222 patients. The test accurately detected H. pylori infection and VacA status, but improvements in the interpretation criteria are required before it can be recommended for determination of CagA status.
authors.affiliation: Department of Medicine, Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Texas, USA.
authors.forename: Chang-Young
authors.initials: CY
authors.lastname: Park
authors.forename: Yong-Kyun
authors.initials: YK
authors.lastname: Cho
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kodama
authors.forename: Hala M T
authors.initials: HM
authors.lastname: El-Zimaity
authors.forename: Michael S
authors.initials: MS
authors.lastname: Osato
authors.forename: David Y
authors.initials: DY
authors.lastname: Graham
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamaoka
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000032:analysis*; D016481:Helicobacter Infections / Q000276:immunology; D016480:Helicobacter pylori / Q000472:pathogenicity*; D006801:Humans; D015151:Immunoblotting; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014774:Virulence; D037521:Virulence Factors / Q000032:analysis*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; D037521:Virulence Factors; C080866:cagA protein, Helicobacter pylori