Mitochondrial creatine kinase with atypical pI values detected in serum of a patient with ovarian hepatoid yolk sac tumor.
Atypical mitochondrial creatine kinase (creatine N-phosphotransferase, CK, EC 2.7.3.2) was detected in the serum of a patient with carcinoma of germ cell origin, probably hepatoid yolk sac tumor. The pI of the oligomeric atypical mitochondrial CK (Mi-CK) was found at the acidic side compared to that of the typical ubiquitous Mi-CK (uMi-CK), while the molecular size of the atypical Mi-CK was similar to that of the typical uMi-CK. The pIs of the oligomeric and the dimeric atypical Mi-CKs became the same as those of the typical uMi-CK upon treatment with 2-mercaptoethanol. Therefore, the atypical Mi-CK was suggested to be an oxidized form of uMi-CK, and the oxidation might have occurred in the mitochondria because the oligomeric atypical Mi-CK had atypical pIs. The physicochemical characteristics of the oxidized uMi-CK were similar to those of the typical uMi-CK.
Kanemitsu
Fusae
F
Clinical Laboratories, Kurashiki Central Hospital, 1-1-1 Miwa, 710-8602, Kurashiki, Japan.
Kageoka
Takeshi
T
Kira
Shohei
S
D003402:Creatine Kinase / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D018240:Endodermal Sinus Tumor / Q000097:blood / Q000201:enzymology*; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008875:Middle Aged; D008928:Mitochondria / Q000201:enzymology*; D010051:Ovarian Neoplasms / Q000097:blood / Q000201:enzymology*
D003402:Creatine Kinase