[Using shock waves for transfer of molecules in cells].
A mixture of human lymphocytes (L1210) and fluorescent marker molecules are subjected to shockwaves in vitro. Due to the transient cavitation generated by the shockwaves, the cells take up the marker molecules. Cavitation is characterized by the bubble collapse times. An electrohydraulic generator XL-1 and a piezoelectric generator PR-II were used; PR-II was more effective. Depending on the pulse energy and number of pulses, up to 70% of the surviving cells took up the molecules. Shockwave-mediated molecule transfer provides a useful tool for the transfer of molecules into cells, which can be used as a research tool in the medical and biotechnological fields. Due to the large penetration potential of shock-waves into the body, the method may be further developed for in vivo transfer of drugs and cell transfection use.
Ueberle
Friedrich
F
Dornier MedTech, Wessling, Deutschland. public@ueberle.de
Delius
Michael
M
Guo
Lei
L
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D003911:Dextrans / Q000493:pharmacokinetics*; D019793:Fluorescein / Q000493:pharmacokinetics*; D006801:Humans; D007939:Leukemia L1210; D008096:Lithotripsy
D003911:Dextrans; D019793:Fluorescein