[Construction and identification of cDNA library of E.coli mRNA with poly(A) tracts].
OBJECTIVE
To construct and identify the cDNA library of E.coli mRNA with poly(A) tracts.


METHODS
The cDNA library of E.coli was constructed by restriction display-PCR (RD-PCR) technique, followed by sequencing and bioinformatics analyses.


RESULTS
cDNA library of E.coli mRNA with poly(A) tracts was successfully constructed, and 66 gene fragments were sequenced.


CONCLUSIONS
The constructed cDNA library of E.coli mRNA with poly(A) tracts contains a low rate of repetition and is of high quality.
Hu
Zi-You
ZY
Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.
Ma
Wen-Li
WL
Wu
Qing-Hua
QH
Zhang
Bao
B
Song
Yan-Bin
YB
Guo
Qiu-Ye
QY
Zheng
Wen-Ling
WL
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015723:Gene Library; D005798:Genes, Bacterial; D026723:Polyadenylation; D012329:RNA, Bacterial / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*
D018076:DNA, Complementary; D012329:RNA, Bacterial; D012333:RNA, Messenger