title: Vaccination of mice against invasive aspergillosis with recombinant Aspergillus proteins and CpG oligodeoxynucleotides as adjuvants.
abstract: In a murine model of invasive pulmonary aspergillosis, dendritic cells (DCs) pulsed with Aspergillus antigens induced the activation of CD4(+) Th1 cells capable of conferring resistance to the infection. Here we show that the combined, local delivery of unmethylated CpG oligodeoxynucleotides (ODNs) and the Asp f 16 Aspergillus allergen resulted in the functional maturation and activation of airway DCs capable of inducing Th1 priming and resistance to the fungus. Therefore, ODNs act as a potent adjuvant for the vaccine-induced protection against the fungus by promoting dominant Th1 response to Aspergillus antigens and allergens.
authors.affiliation: Microbiology section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bozza
authors.forename: Roberta
authors.initials: R
authors.lastname: Gaziano
authors.forename: Grayson B
authors.initials: GB
authors.lastname: Lipford
authors.forename: Claudia
authors.initials: C
authors.lastname: Montagnoli
authors.forename: Angela
authors.initials: A
authors.lastname: Bacci
authors.forename: Paolo
authors.initials: P
authors.lastname: Di Francesco
authors.forename: Viswanath P
authors.initials: VP
authors.lastname: Kurup
authors.forename: Hermann
authors.initials: H
authors.lastname: Wagner
authors.forename: Luigina
authors.initials: L
authors.lastname: Romani
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001228:Aspergillosis / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D001232:Aspergillus fumigatus / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000235:genetics / Q000276:immunology; D005657:Fungal Vaccines; D007249:Inflammation / Q000276:immunology; D008172:Lung Diseases, Fungal / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000627:therapeutic use; D018417:Th1 Cells / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D005656:Fungal Proteins; D005657:Fungal Vaccines; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins