[Determination of rhein in plasma of rat by HPLC/MS].
A HPLC/MS method has been established for the determination of rhein in rat plasma, using an Alltima C18 column (4.6 x 250 mm, 5 microns) and a mixture of ACN-water (60:40) as the mobile phase, at the flow rate of 0.8 ml/min with the column temperature at 25 degrees C. The results showed that the level of rhein distributed in plasma reached the max value 0.5 h after the oral administration, and maintained a relatively high value from 0.25 h to 1.5 h. The method possessed high selectivity and sensitivity.
Zhu
Chenchen
C
New Drug R&D Center, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405.
Zheng
Zhihua
Z
Chen
Zhiliang
Z
Liang
Xi
X
D000818:Animals; D000880:Anthraquinones / Q000097:blood* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D006899:Mixed Function Oxygenases / Q000097:blood* / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D012250:Rheum / Q000737:chemistry*; D012680:Sensitivity and Specificity
D000880:Anthraquinones; D006899:Mixed Function Oxygenases; C020491:rhein