Hodgkin and Reed-Sternberg cell-associated autoantigen CLIP-170/restin is a marker for dendritic cells and is involved in the trafficking of macropinosomes to the cytoskeleton, supporting a function-based concept of Hodgkin and Reed-Sternberg cells.
Little is known about the distribution in normal cells of CLIP-170, a linkage mediator between endocytic vesicles and microtubules, and restin, a splice variant encoded by the same gene and marker for Hodgkin and Reed-Sternberg (HRS) cells of Hodgkin disease. Although only trace amounts of CLIP-170/restin are present in peripheral blood mononuclear cell subpopulations, monocyte-derived dendritic cells (DCs) and interleukin-4 (IL-4) + CD40L-activated B cells express high levels of CLIP-170/restin. CLIP-170/restin colocalizes preferentially with membranes of intermediate macropinocytic vesicles, suggesting a new function of CLIP-170/restin in the trafficking of macropinosomes to the cytoskeleton, which is a crucial step in antigen presentation. The strong expression of CLIP-170/restin in HRS cells, DCs, and activated B cells underscores their functional similarities supporting a function-based concept of HRS cells as professional antigen-presenting cells.
Sahin
Ugur
U
Medizinische Klinik I, Saarland University Medical School, Homburg, Germany.
Neumann
Frank
F
Tureci
Ozlem
O
Schmits
Rudolf
R
Perez
Franck
F
Pfreundschuh
Michael
M
D000938:Antigen-Presenting Cells / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D003599:Cytoskeleton / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism*; D020869:Gene Expression Profiling; D006689:Hodgkin Disease / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins; D010873:Pinocytosis; D016133:Polymerase Chain Reaction; D016539:Reed-Sternberg Cells / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D000951:Antigens, Neoplasm; D015415:Biomarkers; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; C076744:cytoplasmic linker protein 170