Novel N gene-associated, temperature-independent resistance to the movement of tobacco mosaic virus vectors neutralized by a cucumber mosaic virus RNA1 transgene.
The N gene conditions for resistance to Tobacco mosaic virus (TMV) but only below 28 degrees C. However, a TMV-based vector expressing green fluorescent protein (TMV-GFP) showed only limited movement at 33 degrees C in tobacco plants harboring the N gene and other genes cointrogressed from Nicotiana glutinosa. TMV-GFP moved efficiently in tobacco plants that either lacked these genes or that contained the N gene but were transgenic for RNA1 of Cucumber mosaic virus. These findings identified novel temperature-independent resistance to the movement of TMV-GFP which could be neutralized by a different viral transgene. Using the N gene and nahG gene-transgenic tobacco, we show that this novel resistance is manifested specifically by the N gene itself and operates via a pathway independent of salicylic acid.
Canto
Tomas
T
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom.
Palukaitis
Peter
P
D001692:Biological Transport; D017799:Cucumovirus / Q000235:genetics*; D017343:Genes, Plant / Q000502:physiology*; D005822:Genetic Vectors / Q000502:physiology*; D005838:Genotype; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D020365:RNA Helicases / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000502:physiology*; D013696:Temperature; D014026:Nicotiana / Q000235:genetics* / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000235:genetics / Q000502:physiology*; D019076:Transgenes
D008164:Luminescent Proteins; D012367:RNA, Viral; D049452:Green Fluorescent Proteins; D020365:RNA Helicases