Cloning and identification of a cDNA that encodes a novel human protein with thrombospondin type I repeat domain, hPWTSR.
A cDNA was isolated from the fetal brain cDNA library by high throughput cDNA sequencing. The 2390 bp cDNA with an open reading fragment (ORF) of 816 bp encodes a 272 amino acids putative protein with a thrombospondin type I repeat (TSR) domain and a cysteine-rich region at the N-terminus, so it is named hPWTSR. We used Northern blot detected two bands with length of about 3 kb and 4 kb respectively, which expressed in human adult tissues with different intensities. The expression pattern was verified by RT-PCR, revealing that the transcripts were expressed ubiquitously in fetal tissues and human tumor tissues too. However, the transcript was detected neither in ovarian carcinoma GI-102 nor in lung carcinoma LX-1. Blast analysis against NCBI database revealed that the new gene contained at least 5 exons and located in human chromosome 6q22.33. Our results demonstrate that the gene is a novel member of TSR supergene family.
Chen
Jin-Zhong
JZ
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, 200433, People's Republic of China.
Wang
Shu
S
Tang
Rong
R
Yang
Quan-Sheng
QS
Zhao
Enpeng
E
Chao
Yaoqiong
Y
Ying
Kang
K
Xie
Yi
Y
Mao
Yu-Min
YM
D000328:Adult; D020816:Amino Acid Motifs; D001923:Brain Chemistry / Q000235:genetics; D002896:Chromosomes, Human, Pair 6; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005091:Exons; D005333:Fetus; D006801:Humans; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid; D019700:Thrombospondin 1 / Q000235:genetics*; D019699:Thrombospondins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured; D000098907:R-Spondins
D018076:DNA, Complementary; D012333:RNA, Messenger; D019700:Thrombospondin 1; D019699:Thrombospondins; C475438:RSPO3 protein, human; D000098907:R-Spondins