title: Unique features of fowl adenovirus 9 gene transcription.
abstract: We examined the transcriptional organization of fowl adenovirus 9 (FAdV-9) and analyzed temporal transcription profiles of its early and late mRNAs. At least six early and six late transcriptional regions were identified for FAdV-9. Extensive splicing was observed in all FAdV-9 early transcripts examined. Sequence analysis of the cDNAs representing the early proteins identified untranslated leader sequences, precise locations of splice donor and acceptor sites, as well as polyadenylation signals and polyadenylation sites. A unique characteristic, compared to other adenoviruses, was the detection by RT-PCR of multiple transcripts specific for each of five late genes (protein III, pVII, pX, 100K, and fiber), suggesting that FAdV-9 late transcripts undergo more extensive splicing than reported for other adenoviruses.
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, College of Biological Science, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: Davor
authors.initials: D
authors.lastname: Ojkic
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Krell
authors.forename: Eva
authors.initials: E
authors.lastname: Nagy
mesh_terms: D017456:Adenovirus Early Proteins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000259:Aviadenovirus / Q000235:genetics*; D001483:Base Sequence; D002213:Capsid / Q000378:metabolism; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D017456:Adenovirus Early Proteins; D036022:Capsid Proteins; D012333:RNA, Messenger; D014764:Viral Proteins; C018315:hexon capsid protein, Adenovirus