title: Genome analysis of dengue type-1 virus isolated between 1990 and 2001 in Brazil reveals a remarkable conservation of the structural proteins but amino acid differences in the non-structural proteins.
abstract: We have investigated the genetic diversity of dengue type-1 (DEN-1) virus in Brazil. The full nucleotide sequences of three DEN-1 virus isolated from DEN fever (DF) and DEN hemorrhagic fever patients in northeastern Brazil in 1997 (BR/97) and one from a DF patient in the south of Brazil in 2001 (BR/01) were compared to that of the reference strain BR/90 obtained in the city of Rio de Janeiro in 1990. Sequence analysis showed that the structural proteins were remarkably conserved between all isolates. A total of 27 amino acid changes occurred throughout the non-structural proteins. Among them, nine amino acid substitutions were specific of BR/97 and BR/01 isolates, indicating that in situ evolution of these strains had occurred. Within the BR/97 and BR/01 samples, some amino acid substitutions have been previously identified in DEN-1 virus strains sequenced so far, suggesting that recombination events might have occurred.
authors.affiliation: Instituto de Biologia Molecular do Paraná, Curitiba, Paraná and Fundação Oswaldo Cruz, R J, Rio de Janeiro, Brazil. clsantos@tecpar.br
authors.forename: Claudia Nunes Duarte
authors.initials: CN
authors.lastname: dos Santos
authors.forename: Carlos Fernando S
authors.initials: CF
authors.lastname: Rocha
authors.forename: Marli
authors.initials: M
authors.lastname: Cordeiro
authors.forename: Stênio P
authors.initials: SP
authors.lastname: Fragoso
authors.forename: Felix
authors.initials: F
authors.lastname: Rey
authors.forename: Vincent
authors.initials: V
authors.lastname: Deubel
authors.forename: Philippe
authors.initials: P
authors.lastname: Desprès
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001938:Brazil; D017124:Conserved Sequence; D003715:Dengue / Q000821:virology; D003716:Dengue Virus / Q000235:genetics* / Q000302:isolation & purification; D019143:Evolution, Molecular; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D019595:Severe Dengue; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics*; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins