title: Synthesis of chloroplast galactolipids in apicomplexan parasites.
abstract: Monogalactosyldiacylglycerol and digalactosyldiacylglycerol are major chloroplast lipids of algae and land plants and are synthesized within the plastid envelope. Here we report that in Toxoplasma gondii and Plasmodium falciparum lysates, radiolabeled UDP-galactose is incorporated into monogalactosylcerebrosides, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol due to distinct enzymological activities. Furthermore, DGDG is immunologically detected in apicomplexans.
authors.affiliation: Laboratoire de Physiologie Cellulaire Végétale, UMR 5019 CNRS-CEA-Université Joseph Fourier, Département Réponse et Dynamique Cellulaire, CEA-Grenoble, 38054 Grenoble Cedex 9, France. emarechal@cea.fr
authors.forename: Eric
authors.initials: E
authors.lastname: Maréchal
authors.forename: Nahid
authors.initials: N
authors.lastname: Azzouz
authors.forename: Cristiana Santos
authors.initials: CS
authors.lastname: de Macedo
authors.forename: Maryse A
authors.initials: MA
authors.lastname: Block
authors.forename: Jean E
authors.initials: JE
authors.lastname: Feagin
authors.forename: Ralph T
authors.initials: RT
authors.lastname: Schwarz
authors.forename: Jacques
authors.initials: J
authors.lastname: Joyard
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002736:Chloroplasts / Q000378:metabolism*; D038983:Galactolipids / Q000096:biosynthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010963:Plasmodium falciparum / Q000378:metabolism*; D014122:Toxoplasma / Q000378:metabolism*
chemical_list: D038983:Galactolipids; C007388:digalactosyldiacylglycerol; C009909:monogalactosyldiacylglycerol