Peptide-beta2-microglobulin-MHC fusion molecules bind antigen-specific T cells and can be used for multivalent MHC-Ig complexes.
Recombinant soluble MHC molecules are widely used for visualization, activation and inhibition of antigen-specific immune responses. Using a genetic approach, we have generated two novel peptide-beta2-microglobulin-MHC constructs. We have linked the MHC molecule with the peptide of interest, without limiting the recognition by the cognate TCR. This molecule can also be joined with the IgG heavy chain resulting in a dimeric MHC-Ig fusion protein. These molecules bind antigen-specific T cells with high specificity and sensitivity, therefore, providing a valuable tool for detection as well as enrichment of antigen-specific T cells.
Greten
Tim F
TF
Abteilung Gastroenterologie, Hepatologie und Endokrinologie, Medizinische Hochschule Hannover, FZ-Oststadt, Raum 310, Pasteurallee 5, Germany. greten.tim@mh-hannover.de
Korangy
Firouzeh
F
Neumann
Gunnar
G
Wedemeyer
Heiner
H
Schlote
Karola
K
Heller
Astrid
A
Scheffer
Stephan
S
Pardoll
Drew M
DM
Garbe
Annette I
AI
Schneck
Jonathan P
JP
Manns
Michael P
MP
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D019556:COS Cells; D002522:Chlorocebus aethiops; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D015789:HLA-A2 Antigen / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D001613:beta 2-Microglobulin / Q000235:genetics / Q000276:immunology*
D018984:Epitopes, T-Lymphocyte; D015789:HLA-A2 Antigen; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D001613:beta 2-Microglobulin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D004247:DNA