Demonstration of Anaplasma (Ehrlichia) platys inclusions in peripheral blood platelets of a dog in Japan.
A free-roaming dog in Okinawa island showed Anaplasma (Ehrlichia) platys-like inclusions within the platelets of peripheral blood samples. The inclusions were positive in indirect fluorescence test with anti-A. phagocytophila serum. The platelet count of the dog was 170,000 microl(-1). The sequence analysis of the 16S rRNA, citrate synthase and heat shock protein genes of DNA from the infected platelets confirmed that the inclusions were A. platys. This is the first detection of A. platys inclusions in dogs in Japan.
Inokuma
Hisashi
H
Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan. inokuma@yamaguchi-u.ac.jp
Fujii
Kaori
K
Matsumoto
Koutaro
K
Okuda
Masaru
M
Nakagome
Kenji
K
Kosugi
Ryusei
R
Hirakawa
Munetaka
M
Onishi
Takafumi
T
D000709:Anaplasma / Q000235:genetics / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000097:blood / Q000469:parasitology*; D000818:Animals; D001792:Blood Platelets / Q000469:parasitology*; D004269:DNA, Bacterial / Q000235:genetics; D004283:Dog Diseases / Q000097:blood / Q000469:parasitology*; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D002479:Inclusion Bodies / Q000469:parasitology; D007564:Japan; D010976:Platelet Count / Q000662:veterinary; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S