Transfusion of pooled buffy coat platelet components prepared with photochemical pathogen inactivation treatment: the euroSPRITE trial.
A nucleic acid-targeted photochemical treatment (PCT) using amotosalen HCl (S-59) and ultraviolet A (UVA) light was developed to inactivate viruses, bacteria, protozoa, and leukocytes in platelet components. We conducted a controlled, randomized, double-blinded trial in thrombocytopenic patients requiring repeated platelet transfusions for up to 56 days of support to evaluate the therapeutic efficacy and safety of platelet components prepared with the buffy coat method using this pathogen inactivation process. A total of 103 patients received one or more transfusions of either PCT test (311 transfusions) or conventional reference (256 transfusions) pooled, leukoreduced platelet components stored for up to 5 days before transfusion. More than 50% of the PCT platelet components were stored for 4 to 5 days prior to transfusion. The mean 1-hour corrected count increment for up to the first 8 test and reference transfusions was not statistically significantly different between treatment groups (13,100 +/- 5400 vs 14,900 +/- 6200, P =.11). By longitudinal regression analysis for all transfusions, equal doses of test and reference components did not differ significantly with respect to the 1-hour (95% confidence interval [CI], -3.1 to 6.1 x 10(9)/L, P =.53) and 24-hour (95% CI, -1.3 to 6.5 x 10(9)/L, P =.19) posttransfusion platelet count. Platelet transfusion dose, pretransfusion storage duration, and patient size were significant covariates (P <.001) for posttransfusion platelet counts. Clinical hemostasis, hemorrhagic adverse events, and overall adverse events were not different between the treatment groups. Platelet components prepared with PCT offer the potential to further improve the safety of platelet transfusion using technology compatible with current methods to prepare buffy coat platelet components.
van Rhenen
Dick
D
Sanquin Blood Bank South West Region, Rotterdam, The Netherlands.
Gulliksson
Hans
H
Cazenave
Jean-Pierre
JP
Pamphilon
Derwood
D
Ljungman
Per
P
Klüter
Harald
H
Vermeij
Hans
H
Kappers-Klunne
Mies
M
de Greef
Georgine
G
Laforet
Michel
M
Lioure
Bruno
B
Davis
Kathryn
K
Marblie
Stephane
S
Mayaudon
Veronique
V
Flament
Jocelyne
J
Conlan
Maureen
M
Lin
Lily
L
Metzel
Peyton
P
Buchholz
Don
D
Corash
Laurence
L
D000328:Adult; D001424:Bacterial Infections / Q000517:prevention & control* / Q000635:transmission; D001787:Blood Group Incompatibility; D001793:Blood Preservation; D004311:Double-Blind Method; D017707:Erythrocyte Transfusion; D005260:Female; D011564:Furocoumarins; D006470:Hemorrhage / Q000453:epidemiology; D006487:Hemostasis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010777:Photochemistry; D017319:Photosensitizing Agents; D010976:Platelet Count; D017713:Platelet Transfusion / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*; D013921:Thrombocytopenia / Q000628:therapy; D013997:Time Factors; D014466:Ultraviolet Rays; D014777:Virus Diseases / Q000517:prevention & control* / Q000635:transmission
D011564:Furocoumarins; D017319:Photosensitizing Agents; C118577:amotosalen