title: Molecular analysis of sarcoidosis tissues for mycobacterium species DNA.
abstract: We performed polymerase chain reaction analysis, for Mycobacterium species 16S rRNA, rpoB, and IS6110 sequences, on 25 tissue specimens from patients with sarcoidosis and on 25 control tissue specimens consisting of mediastinal or cervical lymph nodes and lung biopsies. Mycobacterium species 16S rRNA sequences were amplified from 12 (48%) rpoB sequences and from 6 (24%) of the sarcoidosis specimens. In total, 16S rRNA or rpoB sequences were amplified from 15 sarcoidosis specimens (60%) but were not detected in any of the control tissues (p=0.00002, chi square). In three specimens, the sequences resembled Mycobacterium species other than M. tuberculosis. All specimens with sequences consistent with M. tuberculosis were negative for IS6110. We provide evidence that one of a variety of Mycobacterium species, especially organisms resembling M. tuberculosis, is found in most patients with sarcoidosis.
authors.affiliation: Vanderbilt University School of Medicine and Veteran Affairs Medical Center, Nashville, Tennessee 37232, USA. wonder.drake@mcmail.vanderbilt.edu
authors.forename: Wonder Puryear
authors.initials: WP
authors.lastname: Drake
authors.forename: Zhiheng
authors.initials: Z
authors.lastname: Pei
authors.forename: David T
authors.initials: DT
authors.lastname: Pride
authors.forename: Robert D
authors.initials: RD
authors.lastname: Collins
authors.forename: Timothy L
authors.initials: TL
authors.lastname: Cover
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Blaser
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D009161:Mycobacterium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D012507:Sarcoidosis / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S