title: Stimulatory effect of puerarin on alpha1A-adrenoceptor to increase glucose uptake into cultured C2C12 cells of mice.
abstract: Effects of puerarin, an active principle contained in the roots of Pueraria lobata (Leguminosae), on the regulation of glucose metabolism in an insulin deficient state were investigated in cultured myoblast C 2 C 12 cells using glucose uptake as indicator. Puerarin enhanced the uptake of radioactive glucose into C 2 C 12 cells in a concentration-dependent manner, which was abolished by prazosin pretreatment. Activation of alpha 1 -adrenoceptors by puerarin was further indicated by the displacement of [ 3H]prazosin binding in C 2 C 12 cells. The stimulatory action of puerarin on glucose uptake was also reduced in C 2 C 12 cells pre-incubated with the antagonists, both WB 4101 and RS 17 056, at concentrations sufficient to block alpha 1A -adrenoceptor (alpha 1A -AR). An activation of alpha 1A -AR seems responsible for the action of puerarin in C 2 C 12 cells. Pharmacological inhibition of phospholipase C (PLC) by U73312 resulted a concentration-dependent decrease of puerarin-stimulated glucose uptake in C 2 C 12 cells. This inhibition of glucose uptake by U73122 was specific because the inactive congener, U73343, failed to block puerarin-stimulated glucose uptake. Moreover, both chelerythrine and GF 109203X diminished the action of puerarin at concentration sufficient to inhibit protein kinase C (PKC). The obtained data suggest that an activation of alpha 1A -AR by puerarin in C 2 C 12 cells may increase the glucose uptake via the PLC-PKC pathway.
authors.affiliation: Department of Surgery, Mackay Memorial Hospital, Taipei City, Taiwan, R.O.C.
authors.forename: Hsi-Hsien
authors.initials: HH
authors.lastname: Hsu
authors.forename: Cheng-Kuei
authors.initials: CK
authors.lastname: Chang
authors.forename: Hui-Chen
authors.initials: HC
authors.lastname: Su
authors.forename: I-Min
authors.initials: IM
authors.lastname: Liu
authors.forename: Juei-Tang
authors.initials: JT
authors.lastname: Cheng
mesh_terms: D000818:Animals; D002460:Cell Line / Q000378:metabolism; D003920:Diabetes Mellitus / Q000517:prevention & control; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D007529:Isoflavones / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D032446:Myoblasts / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D018517:Plant Roots; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D029908:Pueraria; D011869:Radioligand Assay; D018340:Receptors, Adrenergic, alpha-1 / Q000187:drug effects* / Q000378:metabolism; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors
chemical_list: D007004:Hypoglycemic Agents; D007529:Isoflavones; D010936:Plant Extracts; D018340:Receptors, Adrenergic, alpha-1; D011493:Protein Kinase C; D010738:Type C Phospholipases; D005947:Glucose; C033607:puerarin