Interleukin-10 expression after intramuscular DNA electrotransfer: kinetic studies.
Transfected muscle can be used as a secreting tissue for therapeutic proteins. Skeletal muscle transfection is increased by suitable electric pulse application (electrotransfer). We and others had shown that electrotransfer of interleukin-10 encoding plasmid is an effective strategy in animal models of chronic diseases such as myocarditis, atherosclerosis, or rheumatoid arthritis. In the present work, we have studied murine interleukin-10 production and secretion after i.m. electrotransfer. In immunocompetent mice, serum and muscle mIL-10 levels were enhanced by electrotransfer. Serum mIL-10 concentration reached rapidly a peak level 2 days after electrotransfer. It then decreased to background at day 14. Muscle mIL-10 mRNA and protein remained more stable, being detectable up to 84 days after electrotransfer. A boost reinjection led to similar high level of circulating mIL-10. The fast decrease of serum mIL-10 was not observed in SCID mice.
Deleuze
Virginie
V
UMR 7001 CNRS/ENSCP/Gencell S.A., Centre de Recherche de Vitry-Alforville, 13 quai Jules Guesde, BP 14, 94403, Vitry-sur-Seine Cedex, France.
Scherman
Daniel
D
Bureau
Michel F
MF
D000818:Animals; D004558:Electric Stimulation; D005260:Female; D018014:Gene Transfer Techniques; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics*; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D018482:Muscle, Skeletal / Q000473:pathology; D009132:Muscles / Q000473:pathology; D010957:Plasmids / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
D012333:RNA, Messenger; D016753:Interleukin-10