Patterned polymer surfaces for cell culture applications.
We studied the physico/chemical effects of deep UV irradiation of polystyrene, PMMA and polycarbonate with respect to cell adhesion and protein immobilization. Photochemical modifications of the polymer surfaces yielded unstable peroxides and carboxylic acid groups. Patterned enzyme and antibody adsorbates were realized by coupling via carbodiimid activation of the COOH-moities. Hepatoma cells (HepG2) and fibroblasts (L929) adhered in the presence of serum proteins in the culture medium on the irradiated regions of the substrate without any further treatment.
Welle
A
A
Institut für Medizintechnik und Biophysik, Forschungszentrum Karlsruhe GmbH, Deutschland. Alexander.Welle@imb.fzk.de
Gottwald
E
E
Weibezahn
K F
KF
D002448:Cell Adhesion / Q000528:radiation effects*; D002460:Cell Line; D006801:Humans; D011076:Polycarboxylate Cement; D011108:Polymers; D019904:Polymethyl Methacrylate; D011137:Polystyrenes; D023822:Tissue Engineering / Q000295:instrumentation*; D014466:Ultraviolet Rays
D011076:Polycarboxylate Cement; D011108:Polymers; D011137:Polystyrenes; C005506:polycarbonate; D019904:Polymethyl Methacrylate