Proteolytic processing of chromogranins is modified in brains of transgenic mice.
In normal brain, secretogranin II and chromogranins A and S are extensively converted by endopeptidases to the peptides secretoneurin, GE-19, and PE-11, respectively. After genetic knockout of PC2 or its helper protein 7B2, but not after mutation of carboxypeptidase E, endoproteolytic processing decreased, as indicated by appearance of intermediate-sized processing products.
Laslop
Andrea
A
Department of Pharmacology, University of Innsbruck, A-6020 Innsbruck, Austria. andrea.laslop@uibk.ac.at
Becker
Alexandra
A
Lindberg
Iris
I
Fischer-Colbrie
Reiner
R
D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000502:physiology; D001921:Brain / Q000378:metabolism*; D043423:Carboxypeptidase H; D002268:Carboxypeptidases / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002864:Chromogranins / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000502:physiology; D051859:Neuroendocrine Secretory Protein 7B2; D010455:Peptides; D010907:Pituitary Hormones / Q000235:genetics* / Q000502:physiology; D045706:Proprotein Convertase 2; D043484:Proprotein Convertases; D013381:Subtilisins / Q000235:genetics* / Q000502:physiology
D002864:Chromogranins; D009419:Nerve Tissue Proteins; D051859:Neuroendocrine Secretory Protein 7B2; D010455:Peptides; D010907:Pituitary Hormones; D002268:Carboxypeptidases; D010450:Endopeptidases; D043423:Carboxypeptidase H; D043484:Proprotein Convertases; D013381:Subtilisins; D045706:Proprotein Convertase 2; D016282:Aspartic Acid Endopeptidases