Circulation and distribution of autotransfused fresh, liquid-preserved and cryopreserved baboon platelets.
OBJECTIVE
Studies were carried out in five healthy male baboons to determine the 111indium oxine (111In-oxine) survival of autologous fresh, liquid-preserved and cryopreserved platelets. Simultaneous organ-distribution studies were performed to determine the percentage uptake of platelets by the spleen and/or liver.


METHODS
Each of five baboons was transfused, on three different occasions, with autologous fresh platelets stored at 22 degrees C for 18 h, liquid-preserved platelets stored at 22 degrees C for 5 days and washed previously frozen platelets, labelled with 111In-oxine.


RESULTS
In vivo recovery at 2 h was 81% for the fresh platelets, 54% for the previously frozen platelets and 44% for the 5-day-old liquid-preserved platelets. The weighted mean life span was 5.4 days for fresh platelets, 4.2 days for previously frozen platelets and 2 days for liquid preserved platelets. Increased radioactivity was detected over the liver 2 h after transfusion for both the previously frozen and liquid-preserved platelets.


CONCLUSIONS
Cryopreserved platelets and liquid-preserved platelets stored at 22 degrees C for 5 days had reduced survival 2 h post-transfusion and reduced life span values compared to fresh platelets. In addition, the finding of increased radioactivity over the liver in the baboons that received cryopreserved and liquid-preserved platelets suggested that the liver was the site for removal of the non-viable platelets.
Valeri
C R
CR
Naval Blood Research Laboratory, Boston University School of Medicine, 615 Albany Street, Boston, MA 02118, USA. navblood@bu.edu
Giorgio
A
A
Macgregor
H
H
Ragno
G
G
D000818:Animals; D001775:Blood Circulation; D001792:Blood Platelets / Q000166:cytology*; D001793:Blood Preservation; D001804:Blood Transfusion, Autologous; D002465:Cell Movement; D002470:Cell Survival; D016922:Cellular Senescence; D015925:Cryopreservation; D008099:Liver; D008297:Male; D009942:Organometallic Compounds / Q000493:pharmacokinetics; D015125:Oxyquinoline / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D010215:Papio; D017713:Platelet Transfusion; D011849:Radioactive Tracers; D013154:Spleen
D009942:Organometallic Compounds; D011849:Radioactive Tracers; C032950:indium oxine; D015125:Oxyquinoline