title: [Isolation and identification of cDNA sequence differentially expressed in human lung carcinoma].
abstract: OBJECTIVE
  The molecular mechanism of the pathogenesis of lung carcinoma is unclear; because key genes related to lung carcinoma have not been identified. This study was designed to isolate and identify differentially expressed cDNA sequences in human lung carcinoma for cloning lung carcinoma-related genes.
  
  
  METHODS
  Using mRNA differential display method combined with cloning and reserve Northern dot blot and sequencing and RT-PCR, differentially expressed cDNA sequences were isolated between two lung cancer cell lines, two adult lung carcinoma tissue and paired normal tissue of trachea epithelia.
  
  
  RESULTS
  Sixteen differentially expressed cDNA fragments were isolated, subsequent cloning of sixteen differentially expressed cDNA fragments were confirmed by reverse Northern dot blot and sequencing and BLAST analysis. Two of these were shown to be novel gene sequences that had not been reported. Eight of the remaining cDNA sequences homologize to known genes; additionally two differentially expressed cDNA sequences were confirmed by RT-PCR.
  
  
  CONCLUSIONS
  Using mRNA differentially display, The authors had successfully isolated ten differentially expressed cDNA sequences from human lung carcinoma, these cDNA sequences might be involved in the pathogenesis of lung carcinoma.
authors.affiliation: Laboratory of Tumor Cell Biology, Cancer Research Institute, Xiangya Medical School, Central South University, Changsha 410078, P. R. China.
authors.forename: Hai-long
authors.initials: HL
authors.lastname: Xie
authors.forename: You-jun
authors.initials: YJ
authors.lastname: Li
authors.forename: Zhu-chu
authors.initials: ZC
authors.lastname: Chen
authors.forename: Yong-jun
authors.initials: YJ
authors.lastname: Guan
authors.forename: Chun-mei
authors.initials: CM
authors.lastname: He
mesh_terms: D015152:Blotting, Northern; D018076:DNA, Complementary / Q000096:biosynthesis / Q000302:isolation & purification*; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary