Organic disulfides as a means to generate streak-free two-dimensional maps with narrow range basic immobilized pH gradient strips as first dimension.
Streaking is a severe problem when narrow range basic immobilized pH gradient strips are used as the first dimension of two-dimensional (2-D) electrophoresis. It is demonstrated that this cysteinyl related streaking is eliminated when focusing is done in the presence of hydroxyethyl disulfide (DeStreak). Use of DeStreak also results in 2-D maps with simplified spot patterns and improved reproducibility.
Olsson
Ingmar
I
Proteomics Product Development, Amersham Biosciences, Uppsala, Sweden.
Larsson
Kjell
K
Palmgren
Ronnie
R
Bjellqvist
Bengt
B
D000818:Animals; D004220:Disulfides / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000737:chemistry; D051379:Mice; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*
D004220:Disulfides; D020543:Proteome