Electron tomography: towards visualizing the molecular organization of the cytoplasm.
Electron tomography of thin sections of plastic-embedded biological material provides valuable insights into the organellar organization of cells. With the advent of automated data-acquisition procedures, it has become possible to study radiation-sensitive specimens, such as frozen-hydrated organelles and whole cells. This opens up exciting perspectives for visualizing the molecular organization of the cytoplasm.
Baumeister
Wolfgang
W
Department of Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany. baumeist@biochem.mpg.de
D000818:Animals; D003593:Cytoplasm / Q000648:ultrastructure*; D004583:Electrons; D005615:Freezing; D007091:Image Processing, Computer-Assisted; D007515:Islets of Langerhans / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000648:ultrastructure; D015388:Organelles / Q000648:ultrastructure*; D014054:Tomography / Q000379:methods*