Meiotic chromosomal variation resulting from irradiation of pollen in maize.
The objective of this study was to standardize an induction strategy of chromosome aberrations in maize inbred line L-869. Pollen grains irradiated with 0, 36 and 72 Gy were used for fertilization. Resulting seeds were planted in a greenhouse to assess the number of abnormal meiotic cells. Germination, height, sterility and mortality were verified. Cells with delayed separation of chromosomes, translocation, deficiency, abnormal pairing, later condensation and anaphase bridges were observed. The number of abnormalities increased as the dosage increased but chromosome aberration types were the same regardless of the dosages used. Various chromosome-altered plants were obtained without viability loss
Viccini
Lyderson F
LF
Biology Department, Federal University of Juiz de Fora, Campus Universitário, 36036-330, Juiz de Fora - MG, Brazil. lviccini@icb.ufjf.br
De Carvalho
Carlos R
CR
D002869:Chromosome Aberrations / Q000528:radiation effects*; D035264:Flowers / Q000235:genetics / Q000528:radiation effects; D005720:Gamma Rays; D018525:Germination / Q000528:radiation effects; D008540:Meiosis / Q000528:radiation effects*; D011058:Pollen / Q000235:genetics* / Q000528:radiation effects*; D014178:Translocation, Genetic / Q000528:radiation effects; D003313:Zea mays / Q000235:genetics* / Q000528:radiation effects*