[The change in IL-13 mRNA expression in rat lungs with acute pulmonary injury induced by lipopolysaccharide].
OBJECTIVE
To observe the change in IL-13 mRNA expression in rat lungs with acute lung injury (ALI) induced by lipopolysaccharide (LPS), so as to investigate the significance of anti-inflammatory mechanism in the development of ALI.


METHODS
(1) Different doses (2 approximately 8 mg/kg) of LPS were injected via the tail vein to the rats to establish ALI model. (2) The IL-13 mRNA content in pulmonary tissue was determined by RT-PCR.


RESULTS
(1) Different degrees of ALI was induced in the rats by different doses of LPS. ARDS was produced in the rats by LPS of 6 mg/kg. (2) The IL-13 mRNA expression in the pulmonary tissue could be enhanced by the induction of LPS, especially when its dose was larger than 6 mg/kg.


CONCLUSIONS
(1) Rat ALI model could be produced by LPS injection via the tail vein. (2) LPS in dose of 6 mg/kg was the threshold for the production of rat ARDS. (3) The incidence of rat ARDS might be closely related to the enhanced expression of IL-13 mRNA in rat pulmonary tissue.
Li
Qi
Q
Institute of Respiratory Disease, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, P.R. China.
Qian
Guisheng
G
Zhang
Qing
Q
Xu
Jianyue
J
Long
Yong
Y
Tang
Zhengcai
Z
Gong
Jinqian
J
D000208:Acute Disease; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D018793:Interleukin-13 / Q000096:biosynthesis* / Q000235:genetics; D008070:Lipopolysaccharides; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D012129:Respiratory Function Tests; D012131:Respiratory Insufficiency / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology
D018793:Interleukin-13; D008070:Lipopolysaccharides; D012333:RNA, Messenger