title: Inflammatory response to different endodontic irrigating solutions.
abstract: OBJECTIVE
  The aim of this study was to evaluate the inflammatory response to irrigating solutions injected into the peritoneal cavity of mice.
  
  
  METHODS
  Sixty mice received intra-peritoneal injections of 0.3 mL of 0.5% sodium hypochlorite, 2.0% chlorhexidine digluconate or phosphate buffered saline (PBS, control). Five animals of each group were sacrificed at 4, 24, 48 h and 7 days after the injection. Liquid from the peritoneal cavity of each animal was collected for the total and differential counting of inflammatory cells and protein leakage.
  
  
  RESULTS
  The 0.5% sodium hypochlorite solution group had greater migration of neutrophils and mononuclear cells to the peritoneal cavity from 48 to 168 h (P < 0.05). There was a significant increase in protein leakage to the peritoneal cavity after 4 up to 48 h in the 0.5% sodium hypochlorite group compared to the control group. Protein leakage was similar in all groups at 168 h. The 2.0% chlorhexidine group had similar results to the control group at all time periods.
  
  
  CONCLUSIONS
  The 0.5% sodium hypochlorite solution induced an inflammatory response, however, the 2.0% chlorhexidine digluconate solution did not induce a significant inflammatory response.
authors.affiliation: Department of Endodontics, School of Dentistry of Araraquara, University of the State of São Paulo, Araraquara, SP, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Tanomaru Filho
authors.forename: M R
authors.initials: MR
authors.lastname: Leonardo
authors.forename: L A B
authors.initials: LA
authors.lastname: Silva
authors.forename: F F
authors.initials: FF
authors.lastname: Aníbal
authors.forename: L H
authors.initials: LH
authors.lastname: Faccioli
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000139:chemically induced / Q000473:pathology; D001672:Biocompatible Materials / Q000009:adverse effects*; D002465:Cell Movement; D002710:Chlorhexidine / Q000009:adverse effects / Q000031:analogs & derivatives*; D004202:Disinfectants / Q000009:adverse effects; D007274:Injections, Intraperitoneal; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000473:pathology; D008297:Male; D051379:Mice; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000473:pathology; D010529:Peritoneal Cavity / Q000473:pathology*; D011506:Proteins / Q000032:analysis; D011897:Random Allocation; D012388:Root Canal Irrigants / Q000009:adverse effects*; D012965:Sodium Chloride; D012973:Sodium Hypochlorite / Q000009:adverse effects; D018709:Statistics, Nonparametric; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D004202:Disinfectants; D011506:Proteins; D012388:Root Canal Irrigants; D012965:Sodium Chloride; D012973:Sodium Hypochlorite; C010882:chlorhexidine gluconate; D002710:Chlorhexidine