BDCM: a novel B-cell line with genetic and functional similarity to dendritic cells.
We describe a B-cell line, BDCM, which arose spontaneously from culture of a pheresis product from a patient with M5a myeloid leukaemia. Cell growth was associated with autocrine activation of the signal transducer and activator of transcription 1 (Stat-1) transcription factor. Although the cells expressed several B-cell surface markers and had a rearranged immunoglobin J region, they also exhibited several characteristics associated with dendritic cells. These included extensive surface projections, cross-priming ability and strong T cell-stimulating capability. In addition, the cytokine production profile of BDCM cells was nearly identical to that of mature monocyte-derived dendritic cells.
Kharbanda
Sandhya
S
Department of Pediatrics, Children's Hospital of Pittsburgh, PA, USA.
Salter
Russell D
RD
Dong
Xin
X
Tuma-Warrino
Renee J
RJ
Steinman
Richard A
RA
D000954:Antigens, Surface / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology*; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D013601:T-Lymphocytes / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology / Q000502:physiology
D000954:Antigens, Surface; D016207:Cytokines