Alkane hydroxylase homologues in Gram-positive strains.
We isolated Gram-positive alkane-degraders from soil and a tricking-bed reactor, and show using polymerase chain reaction (PCR) with degenerate alkane hydroxylase primers and Southern blots that most Rhodococcus isolates contain three to five quite divergent homologues of the Pseudomonas putida GPo1 alkB gene. Two Mycobacterium isolates each contain one homologue, however there is no evidence for the presence of alkB homologues in the remaining strains.
van Beilen
Jan B
JB
Institute of Biotechnology, Swiss Federal Institute of Technology (ETH), ETH-Hönggerberg, CH-8093 Zürich, Switzerland.
Smits
Theo H M
TH
Whyte
Lyle G
LG
Schorcht
Susanne
S
Röthlisberger
Martina
M
Plaggemeier
Thorsten
T
Engesser
Karl-Heinrich
KH
Witholt
Bernard
B
D000473:Alkanes / Q000378:metabolism; D015139:Blotting, Southern; D042926:Cytochrome P-450 CYP4A; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D006094:Gram-Positive Bacteria / Q000145:classification / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology
D000473:Alkanes; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D042926:Cytochrome P-450 CYP4A