T-DNA integration into the Arabidopsis genome depends on sequences of pre-insertion sites.
A statistical analysis of 9000 flanking sequence tags characterizing transferred DNA (T-DNA) transformants in Arabidopsis sheds new light on T-DNA insertion by illegitimate recombination. T-DNA integration is favoured in plant DNA regions with an A-T-rich content. The formation of a short DNA duplex between the host DNA and the left end of the T-DNA sets the frame for the recombination. The sequence immediately downstream of the plant A-T-rich region is the master element for setting up the DNA duplex, and deletions into the left end of the integrated T-DNA depend on the location of a complementary sequence on the T-DNA. Recombination at the right end of the T-DNA with the host DNA involves another DNA duplex, 2-3 base pairs long, that preferentially includes a G close to the right end of the T-DNA.
Brunaud
Véronique
V
URGV, UMR en Génomique Végétale, INRA/CNRS/Université Evry-Val d'Essonne, France.
Balzergue
Sandrine
S
Dubreucq
Bertrand
B
Aubourg
Sébastien
S
Samson
Franck
F
Chauvin
Stéphanie
S
Bechtold
Nicole
N
Cruaud
Corinne
C
DeRose
Richard
R
Pelletier
Georges
G
Lepiniec
Loïc
L
Caboche
Michel
M
Lecharny
Alain
A
D024509:3' Flanking Region; D024506:5' Flanking Region; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D018014:Gene Transfer Techniques; D018745:Genome, Plant; D030821:Plants, Genetically Modified; D017385:Sequence Homology
D004247:DNA