title: Effects of hydrogen peroxide on mitochondrial gene expression of intestinal epithelial cells.
abstract: OBJECTIVE
  To study the effects of hydrogen peroxide on mitochondrial gene expression of intestinal epithelial cells in in vitro model of hydrogen peroxide-stimulated SW-480 cells.
  
  
  METHODS
  RNA of hydrogen peroxide-induced SW-480 cells was isolated, and reverse-transcriptional polymerase chain reaction was performed to study gene expression of ATPase subunit 6, ATPase subunit 8, cytochrome c oxidase subunit I (COI), cytochrome coxidase subuit II (COII) and cytochrome c oxidase subunit III (COIII). Mitochondria were isolated and activities of mitochondrial cytochrome c oxidase and ATPase were also measured simultaneously.
  
  
  RESULTS
  Hydrogen peroxide led to differential expression of mitochondrial genes with some genes up-regulated or down-regulated in a dose dependent manner. Differences were very obvious in expressions of mitochondrial genes of cells treated with hydrogen peroxide in a concentration of 400 micromol/L or 4 mmol/L. In general, differential expression of mitochondrial genes was characterized by up-regulation of mitochondrial genes in the concentration of 400 micromol/L and down-regulation in the concentration of 4 mmol/L. In consistence with changes in mitochondrial gene expressions, hydrogen peroxide resulted in decreased activities of cytochrome c oxidase and ATPase.
  
  
  CONCLUSIONS
  The differential expression of mitochondrial genes encoding cytochrome c oxidase and ATPase is involved in apoptosis of intestinal epithelial cells by affecting activities of cytochorme c oxidase and ATPase.
authors.affiliation: Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Cai
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.forename: Guang-Xia
authors.initials: GX
authors.lastname: Xiao
mesh_terms: D000199:Actins / Q000235:genetics; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D017209:Apoptosis; D002460:Cell Line; D004272:DNA, Mitochondrial / Q000235:genetics; D004579:Electron Transport / Q000187:drug effects; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D004272:DNA, Mitochondrial; D012333:RNA, Messenger; D006861:Hydrogen Peroxide; D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases