The role of membrane stage in the interaction of cortisol with hepatocytes.
We studied the effect of polyvinylpyrrolidone-immobilized cortisol on the distribution of glucocorticoid-receptor complexes in hepatocytes. Immobilized cortisol 1.2-1.5-fold increased the number of glucocorticoid-receptor complexes associated with the nuclei in a dose-dependent manner. The transport of glucocorticoid-receptor complexes into hepatocyte nuclei induced by immobilized cortisol is associated with their activation. Moreover, the ratio between nuclear pools of glucocorticoid-receptor complexes resistant to 0.2 and 0.4 M KCl extraction was changed. It was concluded that glucocorticoid binding to specific plasma membrane sites induced further stages of interaction between the hormone and competent cell.
Ogurtsov
S I
SI
Russian Medical State University, Moscow.
Dukhanin
A S
AS
D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology*; D009994:Osmolar Concentration; D011189:Potassium Chloride / Q000494:pharmacology; D011205:Povidone; D011965:Receptors, Glucocorticoid / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; D011189:Potassium Chloride; D011205:Povidone; D006854:Hydrocortisone