PCR detection of DNAs of animal origin in feed by primers based on sequences of short and long interspersed repetitive elements.
PCR primers for the detection of materials derived from ruminants, pigs, and chickens were newly designed on the basis of sequences of the Art2 short interspersed repetitive element (SINE), PRE-1 SINE, and CR1 long interspersed repetitive element (LINE), respectively. These primers amplified the SINE or LINE from total DNA extracted from the target animals and from test feed containing commercial meat and bone meal (MBM). With the primers, detection of Art2, PRE-1, or CR1 in test feed at concentrations of 0.01% MBM or less was possible. This method was suitable for the detection of microcontamination of feed by animal materials.
Tajima
Kiyoshi
K
National Institute of Livestock and Grassland Science, P.O. Box 5, Tsukuba, Norindanchi, Ibaraki 305-0901, Japan. ktajima@affrc.go.jp
Enishi
Osamu
O
Amari
Masahiro
M
Mitsumori
Makoto
M
Kajikawa
Hiroshi
H
Kurihara
Mitsunori
M
Yanai
Satoshi
S
Matsui
Hiroki
H
Yasue
Hiroshi
H
Mitsuhashi
Tadayoshi
T
Kawashima
Tomoyuki
T
Matsumoto
Mitsuto
M
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002645:Chickens; D004247:DNA / Q000032:analysis*; D017931:DNA Primers / Q000032:analysis; D005399:Fishes; D006041:Goats; D006736:Horses; D020071:Interspersed Repetitive Sequences; D008969:Molecular Sequence Data; D011784:Quail; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012756:Sheep; D013552:Swine
D017931:DNA Primers; D004247:DNA