title: First occurrence of transferable extended-spectrum beta-lactamase hydrolyzing cefoperazone in multiresistant nosocomial strains of Klebsiella pneumoniae from two hospitals in Czech and Slovak Republics.
abstract: The occurrence of positive synergy between antibiotic discs of amoxicillin/clavulanate and cefoperazone was registered in two Klebsiella pneumoniae strains, isolated from hospitals in Czech and Slovak Republic, indicating the presence of genes coding for an extended-spectrum beta-lactamase active also against cefoperazone, a broad-spectrum cephalosporin. Sulbactam inhibited the hydrolysis of cefoperazone by cell-free lysates of these strains which substantiates its use in combination with cefoperazone. Resistance to cephalothin, cefotaxime, ceftazidime, cefoperazone, cefepime and aztreonam was transferred from K. pneumoniae isolates to Escherichia coli K-12 3110 and to Proteus mirabilis P-38 recipient strains.
authors.affiliation: Institute of Preventive and Clinical Medicine, Limbová 14, 833 01 Bratislava, Slovak Republic. blahova@upkm.sk
authors.forename: J
authors.initials: J
authors.lastname: Blahová
authors.forename: K
authors.initials: K
authors.lastname: Králiková
authors.forename: V
authors.initials: V
authors.lastname: Krćméry
authors.forename: A
authors.initials: A
authors.lastname: Vaculíková
authors.forename: P
authors.initials: P
authors.lastname: Jezek
mesh_terms: D019980:Amoxicillin-Potassium Clavulanate Combination / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002438:Cefoperazone / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology; D003428:Cross Infection / Q000188:drug therapy; D018153:Czech Republic; D004347:Drug Interactions; D018432:Drug Resistance, Multiple; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D006801:Humans; D006868:Hydrolysis; D007710:Klebsiella Infections / Q000188:drug therapy*; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity; D011513:Proteus mirabilis / Q000187:drug effects / Q000235:genetics; D018154:Slovakia
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D019980:Amoxicillin-Potassium Clavulanate Combination; D002438:Cefoperazone