Use of isolated mature osteoblasts in abundance acts as desired-shaped bone regeneration in combination with a modified poly-DL-lactic-co-glycolic acid (PLGA)-collagen sponge.
Controlled regeneration of bone or cartilage has recently begun to facilitate a host of novel clinical treatments. An osteoblast line, which we isolated is able to form new bone matrix in vivo within 2 days and exhibits a mature osteoblast phenotype both in vitro and in vivo. Using these cells, we show that cuboidal bones can be generated into a predesigned shaped-bone with high-density bone trabeculae when used in combination with a modified poly-DL-lactic-co-glycolic acid (PLGA)-collagen sponge. PLGA coated with collagen gel serves as a good scaffold for osteoblasts. These results indicate that mature osteoblasts, in combination with a scaffold such as PLGA-collagen sponge, show promise for use in a custom-shaped bone regeneration tool for both basic research into osteogenesis and for development of therapeutic applications.
Ochi
Kensuke
K
Department of Pathology, Keio University School of Medicine, Tokyo, Japan.
Chen
Gouping
G
Ushida
Takashi
T
Gojo
Satoshi
S
Segawa
Kaoru
K
Tai
Hitoshi
H
Ueno
Kenju
K
Ohkawa
Hiroyuki
H
Mori
Taisuke
T
Yamaguchi
Akira
A
Toyama
Yoshiaki
Y
Hata
Jun-Ichi
J
Umezawa
Akihiro
A
D020341:Absorbable Implants / Q000639:trends; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology / Q000627:therapeutic use; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D003094:Collagen / Q000494:pharmacology / Q000627:therapeutic use; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005260:Female; D017930:Genes, Reporter; D019344:Lactic Acid / Q000494:pharmacology / Q000627:therapeutic use; D051379:Mice; D008809:Mice, Inbred C3H; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D011100:Polyglycolic Acid / Q000494:pharmacology / Q000627:therapeutic use; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000494:pharmacology / Q000627:therapeutic use; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods*; D016378:Tissue Transplantation / Q000379:methods*
D000954:Antigens, Surface; D001672:Biocompatible Materials; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen