Tubuloside B from Cistanche salsa rescues the PC12 neuronal cells from 1-methyl-4-phenylpyridinium ion-induced apoptosis and oxidative stress.
The neuroprotective effects of tubuloside B, one of the phenylethanoids isolated from the Chinese herbal medicine Cistanche salsa, on 1-methyl-4-phenylpyridinium ion (MPP +)-induced apoptosis and oxidative stress in PC12 neuronal cells were investigated. PC12 cells treated with MPP + underwent apoptotic death as determined by MTT assay, flow cytometry and DNA agarose gel electrophoresis; intracellular accumulation of reactive oxygen species (ROS) was measured by DCFH-DA staining with laser scanning confocal microscopy (LSCM). Simultaneous treatment with tubuloside B markedly attenuated MPP +-induced cytotoxicity, DNA fragmentation, and intracellular accumulation of ROS. These results strongly indicate that tubuloside B prevents MPP +-induced apoptosis and oxidative stress. Tubuloside B may be applied as an antiparkinsonian agent.
Sheng
Guoqing
G
Department of Molecular and Cellular Pharmaoclogy, School of Pharmaceutical Sciences, Peking University, Beijing, China.
Pu
Xiaoping
X
Lei
Li
L
Tu
Pengfei
P
Li
Changling
C
D015655:1-Methyl-4-phenylpyridinium; D000818:Animals; D000978:Antiparkinson Agents / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D031671:Cistanche; D053938:DNA Fragmentation / Q000187:drug effects; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D005960:Glucosides / Q000494:pharmacology* / Q000627:therapeutic use; D018613:Microscopy, Confocal; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells / Q000187:drug effects; D010300:Parkinson Disease / Q000188:drug therapy; D008517:Phytotherapy; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
D000978:Antiparkinson Agents; D005960:Glucosides; D017382:Reactive Oxygen Species; C111523:tubuloside B; D015655:1-Methyl-4-phenylpyridinium