The Drosophila immune defense against gram-negative infection requires the death protein dFADD.
Drosophila responds to Gram-negative infections by mounting an immune response that depends on components of the IMD pathway. We recently showed that imd encodes a protein with a death domain with high similarity to that of mammalian RIP. Using a two-hybrid screen in yeast, we have isolated the death protein dFADD as a molecule that associates with IMD. Our data show that loss of dFADD function renders flies highly susceptible to Gram-negative infections without affecting resistance to Gram-positive bacteria. By genetic analysis we show that dFADD acts downstream of IMD in the pathway that controls inducibility of the antibacterial peptide genes.
Naitza
Silvia
S
Institut de Biologie Moleculaire et Cellulaire, UPR 9022 du CNRS, 15 Rue René Descartes, 67084 Cedex, Strasbourg, France. s.naitza@ibmc.u-strasbg.fr
Rossé
Carine
C
Kappler
Christine
C
Georgel
Philippe
P
Belvin
Marcia
M
Gubb
David
D
Camonis
Jacques
J
Hoffmann
Jules A
JA
Reichhart
Jean Marc
JM
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002352:Carrier Proteins / Q000276:immunology*; D004330:Drosophila / Q000276:immunology* / Q000382:microbiology; D029721:Drosophila Proteins / Q000276:immunology*; D053200:Fas-Associated Death Domain Protein; D005786:Gene Expression Regulation / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology*; D007109:Immunity; D015398:Signal Transduction / Q000276:immunology
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D029721:Drosophila Proteins; C469219:FADD protein, Drosophila; D053200:Fas-Associated Death Domain Protein; C439909:imd protein, Drosophila