title: Real-time analysis of rhythmic gene expression in immortalized suprachiasmatic nucleus cells.
abstract: Immortalized cells derived from the suprachiasmatic nucleus (SCN) retain many properties of the SCN including the capacity to generate circadian rhythms. Stably transfected SCN2.2 cells expressing the human c- promoter linked to a luciferase reporter gene ( /luc) were examined for evidence of transgene responses to stimuli known to induce c- expression and of endogenous rhythmic variation. Bioluminescence-reported transgene expression was induced in SCN2.2 /luc cells following stimulation with fetal bovine serum or KCl. SCN2.2 /luc cells showed 24 h rhythms of bioluminescence with a 9- to 19-fold difference between peak and minimum levels. These results demonstrate that the regulation of /luc transgene expression in SCN2.2 cells is similar to that of the endogenous c- gene in the SCN.
authors.affiliation: Department of Human Anatomy, Texas A and M University Health Science Center, College of Medicine, 238 Reynolds Medical Building, College Station, TX 77843-1114, USA.
authors.forename: Gregg C
authors.initials: GC
authors.lastname: Allen
authors.forename: David J
authors.initials: DJ
authors.lastname: Earnest
mesh_terms: D000818:Animals; D002460:Cell Line; D002940:Circadian Rhythm; D015870:Gene Expression; D016762:Genes, fos; D008156:Luciferases / Q000378:metabolism*; D051381:Rats; D013493:Suprachiasmatic Nucleus / Q000201:enzymology / Q000378:metabolism*; D014162:Transfection
chemical_list: D008156:Luciferases