title: Plasma and neutrophil fatty acid composition in advanced cancer patients and response to fish oil supplementation.
abstract: Metabolic demand and altered supply of essential nutrients is poorly characterised in patients with advanced cancer. A possible imbalance or deficiency of essential fatty acids is suggested by reported beneficial effects of fish oil supplementation. To assess fatty acid status (composition of plasma and neutrophil phospholipids) in advanced cancer patients before and after 14 days of supplementation (12+/-1 g day(-1)) with fish (eicosapentaenoic acid, and docosahexaenoic acid) or placebo (olive) oil. Blood was drawn from cancer patients experiencing weight loss of >5% body weight (n=23). Fatty acid composition of plasma phospholipids and the major phospholipid classes of isolated neutrophils were determined using gas liquid chromatography. At baseline, patients with advanced cancer exhibited low levels (<30% of normal values) of plasma phospholipids and constituent fatty acids and elevated 20 : 4 n-6 content in neutrophil phospholipids. High n-6/n-3 fatty acid ratios in neutrophil and plasma phospholipids were inversely related to body mass index. Fish oil supplementation raised eicosapentaenoic acid and docosahexaenoic acid content in plasma but not neutrophil phospholipids. 20 : 4 n-6 content was reduced in neutrophil PI following supplementation with fish oil. Change in body weight during the supplementation period related directly to increases in eicosapentaenoic acid in plasma. Advanced cancer patients have alterations in lipid metabolism potentially due to nutritional status and/or chemotherapy. Potential obstacles in fatty acid utilisation must be addressed in future trials aiming to improve outcomes using nutritional intervention with fish oils.
authors.affiliation: Department of Agricultural, Food and Nutritional Sciences, University of Alberta, 410 Ag/Forestry Center, Edmonton, Alberta, T6G 2P5 Canada.
authors.forename: V C
authors.initials: VC
authors.lastname: Pratt
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: E
authors.initials: E
authors.lastname: Bruera
authors.forename: J
authors.initials: J
authors.lastname: Mackey
authors.forename: M T
authors.initials: MT
authors.lastname: Clandinin
authors.forename: V E
authors.initials: VE
authors.lastname: Baracos
authors.forename: C J
authors.initials: CJ
authors.lastname: Field
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001481:Basal Metabolism; D015992:Body Mass Index; D001835:Body Weight; D002849:Chromatography, Gas; D004281:Docosahexaenoic Acids / Q000097:blood*; D015118:Eicosapentaenoic Acid / Q000097:blood*; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood*; D009504:Neutrophils / Q000378:metabolism*; D000069463:Olive Oil; D010743:Phospholipids / Q000378:metabolism*; D010938:Plant Oils / Q000008:administration & dosage
chemical_list: D015525:Fatty Acids, Omega-3; D000069463:Olive Oil; D010743:Phospholipids; D010938:Plant Oils; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid