title: Prevalence of streptogramin resistance genes among Enterococcus isolates recovered from retail meats in the Greater Washington DC area.
abstract: The prevalence of streptogramin resistance genes in enterococci recovered from retail poultry in the Greater Washington DC area was examined. Forty-three chicken and 32 turkey retail samples were analysed. Thirty-one non-Enterococcus faecalis enterococcal strains were isolated that displayed MICs of quinupristin-dalfopristin and virginiamycin of > or = 4 mg/L. These included Enterococcus faecium (turkey n = 4, chicken n = 23), Enterococcus gallinarum (turkey n = 2, chicken n = 1) and Enterococcus hirae (chicken n = 1). The presence of streptogramin resistance genes was examined by PCR in all non-E. faecalis isolates. The vat(E) gene was detected in 10/23 chicken E. faecium and from 2/4 turkey E. faecium. No other streptogramin resistance genes were detected by PCR. In addition, erm(B) was detected in all the E. faecium and E. gallinarum found in turkeys and in 7/23 E. faecium found in chickens. The vat(E) gene was transferable by conjugation from only two of the 12 E. faecium isolates (one from chicken and one from turkey). This study suggests that there is a high prevalence of low-level streptogramin resistance among enterococci found in retail poultry and that other, yet to be identified, mechanisms operate in these isolates that confer streptogramin resistance in enterococci.
authors.affiliation: US Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Road, Laurel, MD 20708, USA. ssimjee@cvm.fda.gov
authors.forename: S
authors.initials: S
authors.lastname: Simjee
authors.forename: D G
authors.initials: DG
authors.lastname: White
authors.forename: J
authors.initials: J
authors.lastname: Meng
authors.forename: D D
authors.initials: DD
authors.lastname: Wagner
authors.forename: S
authors.initials: S
authors.lastname: Qaiyumi
authors.forename: S
authors.initials: S
authors.lastname: Zhao
authors.forename: J R
authors.initials: JR
authors.lastname: Hayes
authors.forename: P F
authors.initials: PF
authors.lastname: McDermott
mesh_terms: D000818:Animals; D002645:Chickens / Q000382:microbiology; D004219:District of Columbia; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016983:Enterococcus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D008460:Meat / Q000382:microbiology*; D025361:Streptogramins / Q000494:pharmacology; D014422:Turkeys / Q000382:microbiology
chemical_list: D025361:Streptogramins