[Evaluation of selective plating media for the detection of heat- or freeze-injured Staphylococcus aureus].
The efficacy of Baird-Parker (BP) agar, mannitol-salt-egg yolk (MSEY) agar and mannitol salt (MS) agar in detecting Staphylococcus aureus FRI-100 heated at 52 degrees C for 20 min in 100 mmol/L potassium phosphate buffer was determined. Brain heart infusion agar with 1% pyruvate (BHIP agar) supported the highest recovery of injured cells and was used as the control medium. Of the three selective media, significantly higher recovery of heat-injured cells was observed on BP agar than MSEY agar, and the poorest recovery was observed on MS agar (p < 0.05). Low recovery of unheated cells was obtained for MS compared with other media (p < 0.05). A reduction in populations occurred gradually in reagent-grade water stored for 14 days at -20 degrees C. There was no significant difference between BHIP agar and MS agar in the number of freeze-injured cells recovered from 1 to 14 days.
Onoue
Yoichi
Y
Kanagawa Prefectural Public Health Laboratory: 1-1-1, Nakao, Asahi-ku, Yokohama 241-0815, Japan.
Shindo
Rie
R
Teranishi
Hiroshi
H
Furukawa
Ichiro
I
Hasegawa
Yukie
Y
Maruyama
Tsutomu
T
D001431:Bacteriological Techniques; D003470:Culture Media; D005615:Freezing; D006358:Hot Temperature; D013211:Staphylococcus aureus / Q000302:isolation & purification* / Q000502:physiology
D003470:Culture Media