Phylogenetic analysis of acute bee paralysis virus strains.
Reverse transcription-PCR assays have been established for a quick, sensitive, and specific diagnosis of acute bee paralysis virus (ABPV), a common virus of the honeybee (Apis mellifera), directly from clinical samples. A 3,071-nucleotide fragment of the ABPV genome, which includes the entire capsid polyprotein gene, was amplified from Austrian, German, Polish, and Hungarian ABPV samples and sequenced, and the sequences were compared. The alignment of a smaller fragment with ABPV sequences from the United States and the United Kingdom revealed nucleotide identity rates between 89 and 96%, respectively. Phylogenetic trees which display the molecular relationship between the viruses of different geographic origin were constructed.
Bakonyi
Tamás
T
Department of Microbiology and Infectious Diseases, Faculty of Veterinary Sciences, Szent István University, H-1143 Budapest, Hungary.
Grabensteiner
Elvira
E
Kolodziejek
Jolanta
J
Rusvai
Miklós
M
Topolska
Grazyna
G
Ritter
Wolfgang
W
Nowotny
Norbert
N
D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000821:virology*; D002213:Capsid / Q000737:chemistry; D007304:Insect Viruses / Q000145:classification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015678:Viral Structural Proteins / Q000235:genetics
D015678:Viral Structural Proteins