Recent advances in fluorescence correlation spectroscopy.
Fluorescence correlation spectroscopy is a method in which fluctuations in the fluorescence arising from a very small sample volume are correlated to obtain information about the processes giving rise to the fluctuations. Recent progress has been made in methodologies such as two-photon excitation, photon counting histogram analysis, cross-correlation, image correlation and evanescent excitation. Fluorescence correlation spectroscopy techniques have been applied to several biological processes, including fluorescent protein photodynamics, binding equilibria and kinetics, protein oligomerization, nucleic acid interactions, and membrane and intracellular dynamics.
Thompson
Nancy L
NL
Department of Chemistry, Campus Box 3290, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3290, USA. nlt@unc.edu
Lieto
Alena M
AM
Allen
Noah W
NW
D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000737:chemistry; D017785:Photons; D011506:Proteins / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods* / Q000639:trends
D008164:Luminescent Proteins; D011506:Proteins; D049452:Green Fluorescent Proteins