Synthesis of chloroplast galactolipids in apicomplexan parasites.
Monogalactosyldiacylglycerol and digalactosyldiacylglycerol are major chloroplast lipids of algae and land plants and are synthesized within the plastid envelope. Here we report that in Toxoplasma gondii and Plasmodium falciparum lysates, radiolabeled UDP-galactose is incorporated into monogalactosylcerebrosides, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol due to distinct enzymological activities. Furthermore, DGDG is immunologically detected in apicomplexans.
Maréchal
Eric
E
Laboratoire de Physiologie Cellulaire Végétale, UMR 5019 CNRS-CEA-Université Joseph Fourier, Département Réponse et Dynamique Cellulaire, CEA-Grenoble, 38054 Grenoble Cedex 9, France. emarechal@cea.fr
Azzouz
Nahid
N
de Macedo
Cristiana Santos
CS
Block
Maryse A
MA
Feagin
Jean E
JE
Schwarz
Ralph T
RT
Joyard
Jacques
J
D000818:Animals; D002240:Carbohydrate Sequence; D002736:Chloroplasts / Q000378:metabolism*; D038983:Galactolipids / Q000096:biosynthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010963:Plasmodium falciparum / Q000378:metabolism*; D014122:Toxoplasma / Q000378:metabolism*
D038983:Galactolipids; C007388:digalactosyldiacylglycerol; C009909:monogalactosyldiacylglycerol