title: An enhancer complex confers both high-level and cell-specific expression of the human type X collagen gene.
abstract: Type X collagen expression is restricted to hypertrophic chondrocytes in the endochondral growth plate. Transient transfection of reporter constructs containing the human collagen X promoter into primary growth plate chondrocytes identified a cis-acting positive regulatory DNA element(s) that has cell-specific enhancer properties and binds a nuclear protein expressed specifically in growth plate chondrocytes. Functional disruption of this region results in a significant reduction in the activation of reporter gene transcription. The identified enhancer is a major element controlling both high-level and cell-specific expression of type X collagen gene.
authors.affiliation: Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences, 2.205 Stopford Building, Oxford Road, University of Manchester, M13 9PT, Manchester, UK.
authors.forename: David
authors.initials: D
authors.lastname: Chambers
authors.forename: David A
authors.initials: DA
authors.lastname: Young
authors.forename: Charles
authors.initials: C
authors.lastname: Howard
authors.forename: J Terrig
authors.initials: JT
authors.lastname: Thomas
authors.forename: David S
authors.initials: DS
authors.lastname: Boam
authors.forename: Michael E
authors.initials: ME
authors.lastname: Grant
authors.forename: Gillian A
authors.initials: GA
authors.lastname: Wallis
authors.forename: Raymond P
authors.initials: RP
authors.lastname: Boot-Handford
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D019902:Chondrocytes / Q000378:metabolism; D024121:Collagen Type X / Q000235:genetics*; D004247:DNA; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D011401:Promoter Regions, Genetic
chemical_list: D024121:Collagen Type X; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase