title: Developmental expression of eNOS in postnatal swine mesenteric artery.
abstract: Developmental changes in the expression of endothelial nitric oxide synthase (eNOS) within the mesenteric artery of swine were studied in fetal (110 days postconception/117 days total gestation) and on postnatal days 1, 3, 10, and 30. Subjects in the 1-day-old group were subdivided into fed and nonfed. Transcription of eNOS was determined by real-time PCR, protein expression was evaluated by Western blotting, and hemodynamic and oxygenation parameters were measured within in situ gut loops before and after the administration of N(G)-monomethyl-L-arginine (L-NMMA). The abundance of eNOS mRNA remained steady throughout all ages. In contrast, expression of eNOS protein was twofold greater in the 1-day-old fed subjects compared with fetal or 1-day-old nonfed subjects. eNOS protein expression remained elevated on day 3, increased on day 10, and then declined to a level similar to the day 1 nonfed group by postnatal day 30. Intestinal vascular resistance was 31% lower in the day 1 fed group when compared to the day 1 nonfed group; resistance continued to decline through day 10 but then significantly increased on day 30. We conclude that the expression of eNOS changes within the mesenteric artery during early postnatal development at a posttranscriptional level.
authors.affiliation: Department of Pediatrics, Ohio State University and the Children's Research Institute, Children's Hospital, Columbus, Ohio 43205, USA.
authors.forename: Kristina M
authors.initials: KM
authors.lastname: Reber
authors.forename: Baogen Y
authors.initials: BY
authors.lastname: Su
authors.forename: K Reed
authors.initials: KR
authors.lastname: Clark
authors.forename: Dana L
authors.initials: DL
authors.lastname: Pohlman
authors.forename: Charles E
authors.initials: CE
authors.lastname: Miller
authors.forename: Philip T
authors.initials: PT
authors.lastname: Nowicki
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D015153:Blotting, Western; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005215:Fasting; D005502:Food; D015870:Gene Expression; D005865:Gestational Age; D006439:Hemodynamics; D007422:Intestines / Q000098:blood supply / Q000254:growth & development; D008638:Mesenteric Arteries / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III; D010101:Oxygen Consumption; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D013552:Swine; D014158:Transcription, Genetic; D014655:Vascular Resistance; D019323:omega-N-Methylarginine / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D019323:omega-N-Methylarginine; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III