A maximal effort trial in obese women carrying the beta2-adrenoceptor Gln27Glu polymorphism.
The aim of this study was to observe the relationship of the beta2-adrenoceptor (ADRB2) gene polymorphism Gln27Glu in a group of obese female subjects submitted to an acute physical activity test. Six Glu27/Glu27 obese women were compared with six Gln27/Gln27 obese women (coupled by age, BMI, waist circumference, percentage of fat mass and absence of smoking). These groups were selected after having genotyped 91 obese subjects (BMI>30 kg/m2, age 20-60). Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used to genotype the obese population. The physical activity challenge consisted of a Maximal effort trial (VO2 max) following the Bruce protocol on a walking treadmill. The frequency for the Glu allele was 0.42, which is similar to other Caucasian populations. In basal conditions, subjects with the Glu27/Glu27 genotype showed a higher value of plasma insulin, while circulating glucose levels were similar in both groups. Also, diastolic arterial pressure was higher in the Glu27/Glu27 obese women. After the VO2 max trial, the Glu27 group had a significantly higher respiratory exchange ratio than the Gln27 group suggesting a lower post- exercise fat oxidation. These results provide evidence about a possible impact of the Gln27Glu polymorphism in the ADRB2 gene concerning a better response to exercise in obese Gln27 female subjects, in which basal insulinemia may play a role.
Macho-Azcárate
T
T
Dept of Physiology and Nutrition, University Clinic, University of Navarra, Pamplona, Spain.
Calabuig
J
J
Martí
A
A
Martínez
J A
JA
D001786:Blood Glucose / Q000032:analysis; D001794:Blood Pressure / Q000235:genetics; D015992:Body Mass Index; D004734:Energy Metabolism; D005080:Exercise Test; D005260:Female; D005838:Genotype; D018698:Glutamic Acid / Q000235:genetics; D005973:Glutamine / Q000235:genetics; D006801:Humans; D007328:Insulin / Q000097:blood; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D010101:Oxygen Consumption; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics*; D018709:Statistics, Nonparametric
D001786:Blood Glucose; D007328:Insulin; D018343:Receptors, Adrenergic, beta-2; D005973:Glutamine; D018698:Glutamic Acid