title: Changes in pyridoxal kinase immunoreactivity in the gerbil hippocampus following spontaneous seizure.
abstract: To identify the roles of pyridoxal kinase (PLK) in epileptogenesis and the recovery mechanisms in spontaneous seizure, a chronological and comparative analysis of PLK expression in the gerbil hippocampus was conducted. PLK immunoreactivity in a pre-seizure group of seizure sensitive (SS) gerbils was more strongly detected than that in a seizure resistant (SR) group. The density of PLK immunoreactivity in a 30-min postictal group was significantly lower than that of a pre-seizure group. In a 12 h postictal group, PLK immunodensity recovered to pre-seizure level. The over-expression of PLK in the hippocampus of pre-seizure SS gerbils suggests that PLP play an important role in the modulation of GAD activity and GABA reuptake as mediated by membrane transporter via neurons.
authors.affiliation: Department of Anatomy, College of Medicine, College of Life Science, Hallym University, Kangwon-Do, Chunchon, South Korea. tckang@hallym.ac.kr
authors.forename: Tae Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Seung Kook
authors.initials: SK
authors.lastname: Park
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Sung Jin
authors.initials: SJ
authors.lastname: An
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Bahn
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Oh Shin
authors.initials: OS
authors.lastname: Kwon
authors.forename: Nam In
authors.initials: NI
authors.lastname: Baek
authors.forename: Hyeon Yong
authors.initials: HY
authors.lastname: Lee
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D018891:Dentate Gyrus / Q000201:enzymology / Q000503:physiopathology; D015536:Down-Regulation / Q000502:physiology; D004827:Epilepsy / Q000201:enzymology* / Q000503:physiopathology; D005455:Fluorescent Antibody Technique; D050485:GABA Plasma Membrane Transport Proteins; D005849:Gerbillinae; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D006624:Hippocampus / Q000201:enzymology* / Q000503:physiopathology; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins; D009433:Neural Inhibition / Q000502:physiology; D009474:Neurons / Q000201:enzymology*; D027361:Organic Anion Transporters; D011731:Pyridoxal Kinase / Q000378:metabolism*; D011930:Reaction Time / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D050485:GABA Plasma Membrane Transport Proteins; D007527:Isoenzymes; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D027361:Organic Anion Transporters; D005680:gamma-Aminobutyric Acid; D011731:Pyridoxal Kinase; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1; C401141:glutamate decarboxylase 2