4-n-Nonylphenol and 17-beta estradiol may induce common DNA effects in developing barnacle larvae.
There is a growing concern over the potential effects of environmental endocrine disrupters on both human and wildlife populations. However, to date, minimal research has been conducted to determine the effect of estrogens and xenoestrogens at the DNA level. In this study, we used the random amplified polymorphic DNA (RAPD) assay to evaluate the effects on the genomic DNA of barnacle larvae that had been exposed to 17beta-estradiol (E2) and low concentrations of 4-n-nonylphenol (NP). DNA effects include DNA damage as well as mutations and possibly other effects at the DNA level that can be induced by chemical or physical agents that directly and/or indirectly interact with genomic DNA. Not only did exposure to NP and E2 induce changes in RAPD profiles in the exposed barnacle larvae when compared to control patterns, but also, and more importantly, there were similarities in the RAPD modifications in the exposed populations that had been treated to either chemical. We propose that NP and E2 induced some common DNA effects in barnacle larvae and that these specific modifications in RAPD patterns may arise as a consequence of hot spot DNA damage (e.g. DNA adducts) and/or mutations (point mutations or genomic rearrangements). This could help to explain how xenoestrogens mimic the effects produced by natural estrogens. In conclusion, in the field of endocrine disruption, the study of DNA effects induced by estrogens and/or xenoestrogens warrants further investigation. Indeed, changes at the DNA levcl may be the precursors of some of the numerous effects reported at higher levels of biological organisation such as the feminization of males, developmental abnormalities, and infertility.
Atienzar
Franck A
FA
Plymouth Environmental Research Centre, University of Plymouth, Devon, UK. franck.atienzar@ucb-group.com
Billinghurst
Zoë
Z
Depledge
Michael H
MH
D000818:Animals; D004249:DNA Damage; D004958:Estradiol / Q000009:adverse effects*; D007814:Larva / Q000235:genetics; D010636:Phenols / Q000009:adverse effects*; D001468:Thoracica / Q000187:drug effects / Q000235:genetics*; D014874:Water Pollutants, Chemical / Q000009:adverse effects*; D015262:Xenobiotics / Q000009:adverse effects*
D010636:Phenols; D014874:Water Pollutants, Chemical; D015262:Xenobiotics; D004958:Estradiol; C025256:nonylphenol