Molecular cloning of mouse collectin liver 1.
Collectins are members of the superfamily of vertebrate C-type lectins that contain a collagen-like region, and are involved in first-line host defense. We earlier cloned and characterized a new kind of collectin, collectin liver 1 (CL-L1). In this study, we isolated the mouse homologue of CL-L1 encoding 277 amino acid residues; its deduced protein sequence was 88% identical with human CL-L1. Mouse CL-L1 mRNA was expressed mainly in the liver and stomach, but was found also in muscles, testes, intestines, and embryos. In mouse embryos, the level of CL-L1 mRNA gradually increased with embryonic age. In 16-day-old mouse embryos, CL-L1 mRNA was expressed in the liver, amnion, and visceral yolk sac. The mouse CL-L1 gene, Cll1 was found on chromosome 15 in a region syntenic with human chromosome 8q. CL-L1 was a highly conserved protein in mammals, birds, and fish.
Kawai
Takao
T
Division of Food Microbiology, Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka 537-0025, Japan. kawai@iph.pref.osaka.jp
Suzuki
Yasuhiko
Y
Eda
Souji
S
Kase
Tetsuo
T
Ohtani
Katsuki
K
Sakai
Yoshinori
Y
Keshi
Hiroyuki
H
Fukuoh
Atsushi
A
Sakamoto
Takashi
T
Nozaki
Masami
M
Copeland
Neal G
NG
Jenkins
Nancy A
NA
Wakamiya
Nobutaka
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002021:Buffers; D002875:Chromosomes / Q000235:genetics / Q000648:ultrastructure; D003001:Cloning, Molecular; D037222:Collectins / Q000096:biosynthesis*; D015342:DNA Probes / Q000138:chemical synthesis; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D015723:Gene Library; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013045:Species Specificity
D002021:Buffers; C445459:COLEC10 protein, human; D037222:Collectins; D015342:DNA Probes; D018076:DNA, Complementary; D012333:RNA, Messenger; D011994:Recombinant Proteins