title: Liver receptor homologue-1 mediates species- and cell line-specific bile acid-dependent negative feedback regulation of the apical sodium-dependent bile acid transporter.
abstract: Intestinal reclamation of bile salts is mediated in large part by the apical sodium-dependent bile acid transporter (ASBT). The bile acid responsiveness of ASBT is controversial. Bile acid feeding in mice results in decreased expression of ASBT protein and mRNA. Mouse but not rat ASBT promoter activity was repressed in Caco-2, but not IEC-6, cells by chenodeoxycholic acid. A potential liver receptor homologue-1 (LRH-1) cis-acting element was identified in the bile acid-responsive region of the mouse but not rat promoter. The mouse, but not rat, promoter was activated by LRH-1, and this correlated with nuclear protein binding to the mouse but not rat LRH-1 element. The short heterodimer partner diminished the activity of the mouse promoter and could partially offset its activation by LRH-1. Interconversion of the potential LRH-1 cis-elements between the mouse and rat ASBT promoters was associated with an interconversion of LRH-1 and bile acid responsiveness. LRH-1 protein was found in Caco-2 cells and mouse ileum, but not IEC-6 cells or rat ileum. Bile acid response was mediated by the farnesoid X receptor, as shown by the fact that overexpression of a dominant-negative farnesoid X-receptor eliminated the bile acid mediated down-regulation of ASBT. In addition, ASBT expression in farnesoid X receptor null mice was unresponsive to bile acid feeding. In summary cell line- and species-specific negative feedback regulation of ASBT by bile acids is mediated by farnesoid X receptor via small heterodimer partner-dependent repression of LRH-1 activation of the ASBT promoter.
authors.affiliation: Mount Sinai School of Medicine, New York, New York 10029, USA.
authors.forename: Frank
authors.initials: F
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.forename: Paul A
authors.initials: PA
authors.lastname: Dawson
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Sinal
authors.forename: Ephraim
authors.initials: E
authors.lastname: Sehayek
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Gonzalez
authors.forename: Jan
authors.initials: J
authors.lastname: Breslow
authors.forename: M
authors.initials: M
authors.lastname: Ananthanarayanan
authors.forename: Benjamin L
authors.initials: BL
authors.lastname: Shneider
mesh_terms: D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002635:Chenodeoxycholic Acid / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D029363:Organic Anion Transporters, Sodium-Dependent; D011401:Promoter Regions, Genetic; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000502:physiology*; D012689:Sequence Homology, Nucleic Acid; D027981:Symporters
chemical_list: D002352:Carrier Proteins; C431936:Nr5a2 protein, mouse; D029363:Organic Anion Transporters, Sodium-Dependent; D018160:Receptors, Cytoplasmic and Nuclear; D027981:Symporters; D002635:Chenodeoxycholic Acid; C071908:sodium-bile acid cotransporter