[Tea polyphenol inhibit on growth of human nasopharyngeal carcinoma cell and xenograft in nude mice].
OBJECTIVE
It was reported that tea polyphenol(TP) possess preventive and anticancer effects on various human cancers. However the report about TP against human nasopharyngeal carcinoma(NPC) was very rare. Our previous studies have also suggested that TP has antiproliferative effect and may induce apoptosis in human NPC cell. In order to further explore the antitumor effect, the authors investigated the growth inhibition effect of TP on various human NPC cell lines and xenograft tumors of human NPC in nude mice.


METHODS
Antiproliferative effect of TP against seven human NPC cell lines was tested by MTT method. Antitumor effect of TP was determined using the xenografts models of human NPC cell (CNE2) in nude mice.


RESULTS
The fifty percent inhibition concentration (IC50) of TP against NPC cell lines (NPC/HK1, CNE1, CNE2, HNE1, HNE2, SUNE1, and Fadu cells) were calculated to be 55.83, 98.43, 119.21, 127.74, 158.07, 160.72, and 163.59 micrograms/ml, respectively. Average inhibitory rates of TP against xenograft tumors of human NPC in nude mice were 12.7% (P > 0.05), under the dosage of 5 mg.(kg..d)-1, ig x 18 d. Under the dosages of 10 mg.(kg..d)-1, ig x 18 d and 20 mg.(kg..d)-1, ig x 18 average inhibitory rates were 31.0% and 38.5%, (P < 0.01), respectively.


CONCLUSIONS
The results indicated that TP possessed different antiproliferative effect on seven human NPC cell lines in vitro and on xenograft tumor of human NPC in nude mice.
Feng
Gong-kan
GK
Cancer Institute, Cancer Center, Sun Yet-sen University, Guangzhou 510060, P. R. China.
Xie
Bing-fen
BF
Liu
Zong-chao
ZC
Zhu
Xiao-feng
XF
Ye
Yan-li
YL
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use*; D002455:Cell Division / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D005419:Flavonoids; D006801:Humans; D020128:Inhibitory Concentration 50; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000473:pathology; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000188:drug therapy*; D010636:Phenols / Q000627:therapeutic use*; D011108:Polymers / Q000627:therapeutic use*; D059808:Polyphenols; D013662:Tea / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D013662:Tea