Elimination of Salmonella spp. by lactic acid.
The aim of the present study was to determine the elimination of Salmonella by different lactic acid concentrations in microbiological media and on turkey carcass elements. The average bacteria counts in the control samples without lactic acid were: 1.8 x 10(8), 1.1 x 10(8) and 2.3 x 10(8), for S. Enteritidis, S. Anatum and S. Typhimurium, respectively. The concentration of lactic acid of 0.1% in the agar media completely inhibited the growth of all Salmonella strains. At 0.05% lactic acid concentration, the bacteria count was 2 log cycles lower and at a 0.03% solution it was 1 log cycle lower than that in the respective control samples. However, the examined bacteria developed in the presence of 0.02% and 0.01% lactic acid concentrations and their counts fell into the same log brackets. An analysis of the experimental results obtained from turkey carcass elements immersed in the lactic acid solution showed that the Salmonella identification rate was determined by the bacteria inoculum spread over the turkey carcass surface. The contamination of 10(1) CFU of Salmonella spread onto the turkey carcass was completely eliminated by immersing the carcasses in 1% or 2% lactic acid solutions. The contamination of turkey carcass elements with 10(2) CFU of S. Enteritidis and their immersion in 2% lactic acid solution for 15 min resulted in the reduction of the number of samples with Salmonella compared to the number of control samples with Salmonella. At contaminations of 10(3) CFU on the carcass surfaces, the immersions in 1% and 2% lactic acid solutions did not reduce Salmonella counts.
Mikołajczyk
A
A
Department of Animal Products Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 14, 10-957 Olsztyn, Kortowo II, Poland.
Radkowski
M
M
D000818:Animals; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005516:Food Microbiology; D019344:Lactic Acid / Q000494:pharmacology*; D008460:Meat / Q000382:microbiology; D012475:Salmonella / Q000187:drug effects* / Q000254:growth & development; D014422:Turkeys / Q000382:microbiology
D003470:Culture Media; D019344:Lactic Acid