Different activity of glyoxalase system enzymes in specimens of Sparus auratus exposed to sublethal copper concentrations.
The present study regards possible changes in the activity of glyoxalase system enzymes (glyoxalase I, GI, and glyoxalase II, GII) in tissues (brain, liver and white muscle) of the mediterranean bony fish Sparus auratus after a 20 days exposure to sublethal concentrations (0.1 or 0.5 ppm) of Cu in the marine water and on control untreated animals. The experiments also included measurements of copper concentration in the tissues, as well as of lactate dehydrogenase (LDH) activity, to evaluate possible Cu accumulation and changes in glycolytic activity respectively. Cu accumulation only occurs in the liver. GI, GII and LDH activities kept unchanged in the brain after copper exposure. GI activity in liver and muscle of copper-exposed animals decreases probably for a slackening in the glycolytic rate, as suggested by the lowering of LDH activity. GII activity remains unchanged or increases (liver extract, 0.5 ppm of Cu), maybe to safeguard enough cellular levels of GSH.
Antognelli
Cinzia
C
Department of Experimental Medicine, Division of Cellular and Molecular Biology, University of Perugia, Via del Giochetto, 06122 Perugia, Italy.
Romani
Rita
R
Baldracchini
Francesca
F
De Santis
Alessandra
A
Andreani
Giulia
G
Talesa
Vincenzo
V
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D003300:Copper / Q000493:pharmacokinetics / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007791:Lactoylglutathione Lyase / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D011765:Pyruvaldehyde / Q000378:metabolism; D021541:Sea Bream / Q000378:metabolism*; D013869:Thiolester Hydrolases / Q000378:metabolism*; D000099301:Hydroxyacylglutathione Hydrolase
D003300:Copper; D005978:Glutathione; D007770:L-Lactate Dehydrogenase; D007791:Lactoylglutathione Lyase; D011765:Pyruvaldehyde; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase