Inhibition of enzymes of polyamine biosynthesis by substrate-like O-substituted hydroxylamines.
The biogenic amines spermine, spermidine, and putrescine are essential factors of cell growth and differentiation. To inhibit pyridoxal-5'-phosphate dependent ornithine decarboxylase and pyruvate dependent S-adenosylmethionine decarboxylase, key enzymes of polyamine biosynthesis, a system of substrate-like O-substituted hydroxylamines is suggested. The best of these compounds were active at nanomolar concentrations. High potency and specificity of this type of inhibitors are discussed in terms of structural similarity of E-I and E-S complexes.
Khomutov
A R
AR
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia. alexkhom@genome.eimb.relarn.ru
D012437:Adenosylmethionine Decarboxylase / Q000037:antagonists & inhibitors*; D000818:Animals; D006898:Hydroxylamines / Q000737:chemistry* / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology; D051379:Mice; D008968:Molecular Conformation; D015394:Molecular Structure; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines / Q000378:metabolism*; D051381:Rats; D013095:Spermidine / Q000096:biosynthesis / Q000378:metabolism; D013096:Spermine / Q000096:biosynthesis / Q000378:metabolism; D013379:Substrate Specificity
D006898:Hydroxylamines; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines; D013096:Spermine; D012437:Adenosylmethionine Decarboxylase; D013095:Spermidine