High-performance liquid chromatographic analysis of chrysin derivatives on a Nova-Pak C18 column.
A high-performance liquid chromatographic method has been developed for the separation and quantification of chrysin and synthetic chrysin derivatives (12 chrysin alkyl and 7 chrysin acyl derivatives). The chromatography was performed using a Nova-Pak C18 column. A RP-HPLC was performed by using a binary mixture (MeOH-10 mM H3PO4) as a mobile phase, and the column temperature was maintained at room temperature. A flow rate was 1.0 ml/min, and the effluent was monitored at a wavelenth of 280 nm. The retention times for chrysin acyl and alkyl derivatives were within 10 minutes and 20 minutes, respectively. The absolute recovery of samples were all over 96%. The detection limits were 0.1-18 ng at S/N = 3 ratio.
Kim
Kyoung Soon
KS
College of Pharmacy, Seoul National University, Kwanak-Gu, Korea.
Shin
Joon Su
JS
Park
Youmie
Y
Lee
Sanghyun
S
Kim
Yang Bae
YB
Kim
Bak-Kwang
BK
D002250:Carbon Radioisotopes / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry*
D002250:Carbon Radioisotopes; D005419:Flavonoids; C043561:chrysin