title: Biochemical characterization of TEM-92 extended-spectrum beta-lactamase, a protein differing from TEM-52 in the signal peptide.
abstract: A bla(TEM-92) gene was cloned from a Proteus mirabilis isolate and expressed in Escherichia coli. Production of the enzyme caused reduction of susceptibility to penicillins and narrow- to expanded-spectrum cephalosporins but not to moxalactam and cephamycins. Determination of kinetic parameters with the purified enzyme revealed hydrolysis of expanded-spectrum cephalosporins, while cephamycins, moxalactam, and aztreonam were very poorly or not hydrolyzed. Clavulanate and penicillanic acid sulfones acylated TEM-92 slowly, and deacylation occurred at measurable rates.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biomediche, Università di L'Aquila, Italy.
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Perilli
authors.forename: Bernardetta
authors.initials: B
authors.lastname: Segatore
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: De Massis
authors.forename: Laura
authors.initials: L
authors.lastname: Pagani
authors.forename: Francesco
authors.initials: F
authors.lastname: Luzzaro
authors.forename: Gian Maria
authors.initials: GM
authors.lastname: Rossolini
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Amicosante
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018550:Cephalosporin Resistance / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007700:Kinetics; D010403:Penicillin Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; C445691:TEM-52 beta-lactamase; C434286:beta-lactamase TEM-92; D001618:beta-Lactamases