Multiple genes in human 20q13 chromosomal region are involved in an advanced prostate cancer xenograft.
We analyzed a prostate cancer xenograft derived from a locally advanced tumor using combined cytogenetic, array-based comparative genomic hybridization and expression analyses. This analysis revealed that genes in the 20q13 chromosomal region, CSE1L, ZNF217, MYBL2, and STK15, were significantly overexpressed in this tumor. The expression pattern of these genes was then confirmed in two large human prostate cancer microarray databases. Furthermore, the MYBL2 and STK15 have been significantly overexpressed in prostate metastases, allowing a clear distinction between localized tumors and metastases. Our data suggest these genes to be involved in advanced stages of prostate tumorigenesis and as such, they may serve as markers for tumor progression.
Bar-Shira
Anat
A
Genetic Institute, Tel Aviv Sourasky Medical Center, Tel Aviv 64239, Israel.
Pinthus
Jehonathan H
JH
Rozovsky
Uri
U
Goldstein
Myriam
M
Sellers
William R
WR
Yaron
Yuval
Y
Eshhar
Zelig
Z
Orr-Urtreger
Avi
A
D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D064096:Aurora Kinase A; D064127:Aurora Kinases; D029563:Cellular Apoptosis Susceptibility Protein / Q000096:biosynthesis / Q000235:genetics; D002871:Chromosome Banding; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics*; D030541:Databases, Genetic; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics; D014183:Transplantation, Heterologous
D029563:Cellular Apoptosis Susceptibility Protein; D015534:Trans-Activators; C113698:ZNF217 protein, human; C576917:AURKA protein, human; D064096:Aurora Kinase A; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases