Collecting human spermatozoa onto filters for FISH. Application to the study of extreme oligozoospermia.
OBJECTIVE
To describe a simple method of fluorescence in situ hybridization (FISH) performed on germ cells collected on filters that allows the study of aneuploid sperm frequency in cases of extreme oligozoospermia.


METHODS
The aneuploidy rates for chromosomes X, Y and 18 were analyzed in three groups to test the reliability of the filtration-FISH procedure when compared to controls and to evaluate the frequency of aneuploid spermatozoa in five oligospermic men.


RESULTS
Filtration of semen samples before FISH does not modify the basal rate of aneuploidy even after various sperm dilutions. In oligospermic men, the frequency of gonosomal aneuploidies is significantly increased.


CONCLUSIONS
The filtration-FISH technique is a powerful method of analyzing chromosomal abnormalities in sperm nuclei in men with extreme oligozoospermia.
Siffroi
Jean Pierre
JP
Department of Histology, Biology of Reproduction and Cytogenetics, Tenon Hospital, 4 Rue de la Chine, 75020 Paris, France. jean-pierre.dadoune@tnn.ap-hop-paris.fr
Le Bourhis
Corine
C
Dadoune
Jean Pierre
JP
D002467:Cell Nucleus / Q000473:pathology; D002469:Cell Separation / Q000379:methods*; D002869:Chromosome Aberrations; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D009845:Oligospermia / Q000235:genetics / Q000473:pathology*; D013094:Spermatozoa / Q000473:pathology*