Chiral analysis of biogenic D,L-amino acids derivatized by N-fluorenylmethoxycarbonyl-L-alanyl N-carboxyanhydride using high-performance liquid chromatography.
Nineteen biogenic D,L-amino acids are derivatized with highly reactive N-fluorenylmethoxycarbonyl-L-alanyl N-carboxyanhydride. Using a 0.5M borate buffer at pH 7.5 and acetone, the derivatization of amino acids is completed in 5 min at room temperature. Some of the resulting diastereomeric N-protected dipeptides are successfully separated on an octylsilica stationary phase using 100mM acetate buffer (pH 4.4) and acetonitrile as the eluent.
Zbrozek
Jaroslav
J
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague.
Pumera
Martin
M
Flegel
Martin
M
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005449:Fluorenes / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids