In vitro evaluation of newly developed adsorbent for selective removal of glycosylated low-density lipoprotein.
Chronic hyperglycemia leads to the accumulation of nonenzymatically derived glycosylation products on proteins. Such glycosylation products, especially glycosylated low-density lipoprotein (glc-LDL), have been increasingly recognized as factors in the pathogeneses of diabetic complications. A new adsorbent was developed for the selective removal of glc-LDL from plasma. The adsorbent has dual ligands in order to improve the specific binding affinity for glc-LDL that consisted of boronic acid moiety for the glycosylated site and acrylic acid (AA) moiety for the apolipoprotein B of LDL. The adsorbent was synthesized by copolymerization of 4-vinyl phenyl boronic acid and AA. Five kinds of copolymers having different compositions were prepared and evaluated in terms of glc-LDL in vitro adsorption in human plasma. The adsorption behaviors were different depending on the polymer composition. The adsorbent having the AA composition from 50% to 90% showed very high selectivity for glc-LDL adsorption. The capability of selective adsorption was not impaired in human plasma. These results suggested that the adsorbent would be a promising material for glc-LDL apheresis.
Murabayashi
Shun
S
Department of Biomedical Engineering, Graduate School of Engineering, Hokkaido University, Sapporo, Japan. shun@bme.eng.hokudai.ac.jp
Nishide
Takuji
T
Mitamura
Yoshinori
Y
D000179:Acrylates / Q000737:chemistry; D000327:Adsorption; D001781:Blood Component Removal; D001897:Boronic Acids / Q000737:chemistry; D017127:Glycation End Products, Advanced; D006031:Glycosylation; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000097:blood*; D011108:Polymers; D014753:Vinyl Compounds / Q000737:chemistry; D000093844:Glycated Proteins
D000179:Acrylates; D001897:Boronic Acids; D017127:Glycation End Products, Advanced; D008077:Lipoproteins, LDL; D011108:Polymers; D014753:Vinyl Compounds; C427523:4-vinylphenyl boronic acid; C031797:glycated lipoproteins, LDL; D000093844:Glycated Proteins; C036658:acrylic acid