The effect of H2O2 and tertiary butyl hydroperoxide upon a murine immortal lens epithelial cell line, alphaTN4-1.
The effect of comparable concentrations of H(2)O(2) and tertiary butyl hydroperoxide (TBHP) upon an immortal murine lens epithelial cell line was examined as part of an ongoing effort to delineate differences in the mechanism by which these peroxides cause cell death. Both compounds result in cell death of normal, unconditioned cells within 24hr. It was found that with similar conditions, TBHP conditioned alphaTN4-1 cells survive H(2)O(2) stress while H(2)O(2) conditioned cells are killed by TBHP. To better understand how these peroxides act, their effect upon unconditioned cells has been investigated. Both peroxides cause a rapid loss of GSH and disruption of pump activity as illustrated by (14)C-choline transport and (86)Rb uptake. While H(2)O(2) exposure resulted in extensive DNA damage, TBHP had a minimal effect. DNA damage caused by H(2)O(2) was shown to activate polyADP-ribosyl polymerase (PARP), leading to depletion of NAD and ATP. H(2)O(2) induced cell death could be delayed by addition of 3-aminobenzamide (3AB), an inhibitor of PARP. ATP levels in cells subjected to H(2)O(2) were also maintained by the presence of 3AB. H(2)O(2) stress also disrupted glycolysis and mitochondrial activity but these parameters were not affected by TBHP. TBHP induced cell death, under the relatively mild conditions used in this work, appears to be caused by membrane disruption and loss of a reducing environment.
Spector
Abraham
A
Department of Ophthalmology, College of Physicians & Surgeons, Columbia University, New York, NY 10032, USA. as42@columbia.edu
Ma
Wanchao
W
Sun
Fang
F
Li
Dayu
D
Kleiman
Norman J
NJ
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001549:Benzamides / Q000494:pharmacology; D002460:Cell Line; D004249:DNA Damage / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects; D005978:Glutathione; D006019:Glycolysis; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007903:Lens Capsule, Crystalline / Q000187:drug effects*; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000494:pharmacology*
D001549:Benzamides; D004791:Enzyme Inhibitors; C025160:3-aminobenzamide; D000255:Adenosine Triphosphate; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D005978:Glutathione