title: Neural precursor proliferation and newborn cell survival in the adult rat dentate gyrus are affected by vitamin E deficiency.
abstract: The adult hippocampal neurogenesis is affected by vitamin E deficiency. In the present investigation we examined if neural precursor proliferation, newborn cell survival or both are altered by vitamin E deficiency. 5-Bromo-2'-deoxyuridine (BrdU) was employed as a marker of proliferating cells. BrdU-labelled cells were revealed 1 and 30 days after BrdU administration in order to evaluate proliferation and newborn cell survival, respectively. Cell proliferation decreased in controls from juvenile to adult age, and the decrease was lesser in vitamin E deficiency. Thus we found a higher number of proliferating cells in vitamin E-deficient rats than in age-matched controls at 5 months of age. Comparing the number of BrdU-positive cells between 1 and 30 days after the last BrdU injection revealed a remarkable decrease in all groups; this is the greatest in vitamin E-deficient rats and the lowest in control rats. Consistently cell death in the dentate gyrus, assessed by TUNEL technique, was found to decrease from 1 to 5 months of age, but at 5 months it was significantly higher in vitamin E-deficient rats than in age-matched controls. These data show that vitamin E deficiency enhances neural precursor proliferation and cell death during adult neurogenesis.
authors.affiliation: Institute of Morphological Sciences, University of Urbino, Località Crocicchia-Campus Scientifico, I-61029 Urbino, PU, Italy. s.ciaroni@uniurb.it
authors.forename: Sandra
authors.initials: S
authors.lastname: Ciaroni
authors.forename: Tiziana
authors.initials: T
authors.lastname: Cecchini
authors.forename: Paola
authors.initials: P
authors.lastname: Ferri
authors.forename: Riccardo
authors.initials: R
authors.lastname: Cuppini
authors.forename: Patrizia
authors.initials: P
authors.lastname: Ambrogini
authors.forename: Spartaco
authors.initials: S
authors.lastname: Santi
authors.forename: Serena
authors.initials: S
authors.lastname: Benedetti
authors.forename: Paolo
authors.initials: P
authors.lastname: Del Grande
authors.forename: Stefano
authors.initials: S
authors.lastname: Papa
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine; D002452:Cell Count; D016923:Cell Death / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D018891:Dentate Gyrus / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005526:Food, Formulated; D020287:In Situ Nick-End Labeling; D008297:Male; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D014811:Vitamin E Deficiency / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D024502:alpha-Tocopherol / Q000097:blood
chemical_list: D001973:Bromodeoxyuridine; D024502:alpha-Tocopherol