title: [Immune reaction in the mixed culture of host lymphocytes with allogenic and host epithelial cells].
abstract: OBJECTIVE
  To observe the immune reaction in the mixed culture of host lymphocytes with allogenic and host endothelial cells.
  
  
  METHODS
  The host epithelial cells and lymphocytes from burn patients and allogenic epithelial cells were mix-cultured in different ratios, so as to simulate the local immune micro-environment of host skin island in intermingled skin grafting. In addition, the cells from normal human subjects were also mix-cultured as control. The lymphocyte cpm values were detected by (3)H-TdR and HLA molecules and T cell subgroup were determined by immunohistological technique.
  
  
  RESULTS
  (1) The lymphocyte proliferation reaction could be effectively inhibited by the epithelial cells from burn patients but not from normal control. (2) The inhibition of host lymphocyte proliferation could not be mediated by the HLA-DQ molecules of epithelium from burn patients. (3) The positive expression rate of HLA-DR of epithelia from burn patients was evidently higher that that from normal control (P < 0.05), (4) The CD8 expression of lymphocyte in burn patients was significantly higher than that in normal control (P < 0.01), while the CD4 expression in burn patients was lower than that in normal control (P < 0.01). But there was no obvious difference of the CD3 expression between patients and normal subjects (P > 0.05).
  
  
  CONCLUSIONS
  The lymphocyte proliferation reaction could be obviously inhibited by the host epithelium, which might be related to the specific immune state of the host lymphocytes and epithelium of burn patients.
authors.affiliation: Department of Immunology and Microbiology, The Medical School of Southeast University, Nanjing 210009, Jiang Su Province, P.R. China.
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Shen
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Xia
authors.forename: Xiande
authors.initials: X
authors.lastname: Cai
authors.forename: Jingxia
authors.initials: J
authors.lastname: Xu
authors.forename: Guangyan
authors.initials: G
authors.lastname: Zhou
mesh_terms: D002450:Cell Communication / Q000276:immunology* / Q000502:physiology; D018929:Cell Culture Techniques; D002455:Cell Division; D004847:Epithelial Cells / Q000276:immunology* / Q000502:physiology; D006801:Humans; D008214:Lymphocytes / Q000276:immunology* / Q000502:physiology; D016038:Skin Transplantation / Q000276:immunology*