Comparison of the endogenous ileal and faecal amino acid excretion in the dog (Canis familiaris) and the rat (Rattus rattus) determined under protein-free feeding and peptide alimentation.
The aim of the study was to determine and compare the endogenous ileal excretions of nitrogen and amino acids under protein-free and peptide alimentation by the dog and rat. Two diets were prepared, one that was devoid of protein and the other containing 23% enzyme hydrolysed casein. Chromic oxide was included in the diets as an indigestible marker. A total of 10 mixed breed dogs were fed hourly either a protein-free or enzymatically hydrolysed casein diet for a total of 10 days. A faecal sample was obtained from each dog on day 9 while digesta was obtained from the terminal 20 cm of the ileum directly after euthanasia on day 10. A total of 12 8-week-old Sprague-Dawley rats received the same diets as the dogs. A faecal sample from each rat was obtained on day 7 while ileal digesta samples were obtained on day 8. The endogenous ileal excretions of most amino acids were greater in the dogs and rats that received the enzymatically hydrolysed casein diet compared with those receiving the protein free diet. Whereas the pattern of endogenous amino acid excretion was similar in the rats and dogs, the dogs excreted a significantly greater amount of nitrogen (1.91 vs. 2.27 and 1.63 vs. 4.12 g/kg dry matter intake for the protein-free and peptide alimentation method, respectively) and all amino acids except for glycine, isoleucine and leucine. Endogenous ileal amino acid excretions are higher in dogs compared to omnivorous animals such as rats and pigs but similar to the carnivorous cat.
Hendriks
W H
WH
Institute of Food Nutrition and Human Health, Massey University, Palmerston North, New Zealand. w.hendriks@massey.ac.nz
Sritharan
K
K
Hodgkinson
S M
SM
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002364:Caseins / Q000008:administration & dosage*; D004032:Diet / Q000592:standards / Q000662:veterinary; D004044:Dietary Proteins / Q000008:administration & dosage* / Q000592:standards; D004285:Dogs / Q000378:metabolism*; D005243:Feces / Q000737:chemistry*; D005260:Female; D007082:Ileum / Q000378:metabolism*; D008297:Male; D023421:Models, Animal; D009584:Nitrogen / Q000032:analysis; D011492:Protein Hydrolysates / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000596:Amino Acids; D002364:Caseins; D004044:Dietary Proteins; D011492:Protein Hydrolysates; C026375:trypticase-soy broth; D009584:Nitrogen