title: Cytoadherence of malaria-infected red blood cells involves exposure of phosphatidylserine.
abstract: Phosphatidylserine (PS) is a membrane phospholipid which in intact cells is exclusively localized in the inner leaflet of the lipid bilayer. However, once cells undergo apoptosis or oxidative stress, PS molecules are exposed on the external surface of the cells and this contributes to their adherence to macrophages or endothelial cells. PS exposure on Plasmodium falciparum-infected red cells was determined by flow cytometry using fluorescein-labeled annexin V, which specifically binds to PS. Involvement of exposed PS in the adherence of malaria-infected red cells to endothelial cells was examined by in vitro cytoadherence assays. Infected cells exposed PS on their surface as the intracellular parasites matured to trophozoite and schizont stages. Adherence of malaria-infected cells to CD36, CD36-expressing Chinese hamster ovary cells, thrombospondin, and C32 amelanotic melanoma cells was inhibited by annexin V, whereas ICAM-1- and chondroitin sulfate A-mediated binding was not. Further, PS liposomes and glycerophosphorylserine, but not phosphatidylcholine liposomes and glycerophosphorylcholine, inhibited the binding of infected cells to CD36 and thrombospondin. In conclusion, these results demonstrate that PS exposed on the surface of malaria-infected red cells contributes, in part, to the adherence of P. falciparum-parasitized red cells to CD36 and thrombospondin.
authors.affiliation: Department of Biology, University of California Riverside, 92521, USA.
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Eda
authors.forename: Irwin W
authors.initials: IW
authors.lastname: Sherman
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D018955:CD36 Antigens / Q000378:metabolism; D016466:CHO Cells; D002448:Cell Adhesion / Q000502:physiology*; D006224:Cricetinae; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology* / Q000473:pathology*; D005434:Flow Cytometry; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000494:pharmacology; D016778:Malaria, Falciparum / Q000097:blood*; D018328:Melanoma, Amelanotic / Q000378:metabolism; D018613:Microscopy, Confocal; D010718:Phosphatidylserines / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D010963:Plasmodium falciparum / Q000254:growth & development*; D019699:Thrombospondins / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D017304:Annexin A5; D018955:CD36 Antigens; D008081:Liposomes; D010718:Phosphatidylserines; D019699:Thrombospondins