5'-p-Fluorosulfonylbenzoyl adenosine inhibits progesterone synthesis in human placental mitochondria.
The human placental mitochondria have an ATP-diphosphohydrolase (apyrase) activity. In this paper we characterized the effect of 5'-p-fluorosulfonylbenzoyl adenosine (FSBA) on placental apyrase, and its repercussion on progesterone synthesis and oxygen consumption. Apyrase activity was inhibited by FSBA. Nucleosides tri- and diphosphates protected against FSBA inactivation, but divalent cations did not, indicating that FSBA attaches itself to an ATP-binding site of apyrase. In mitochondria, the inactivation of apyrase by FSBA was associated with inhibition of progesterone synthesis. Also, the oxygen consumption induced by ATP but not by ADP, was inhibited, clearly showing that FSBA exclusively inactivated the apyrase in human placental mitochondria. It is concluded that the apyrase activity is closely related to progesterone synthesis, probably associated with the cholesterol transport between mitochondrial membranes.
Flores-Herrera
O
O
Departamento de Bioqui;mica, Facultad de Medicina, Universidad Nacional Autónoma de México. Apartado Postal 70-159, 04510, D.F., Mexico, Mexico.
Uribe
A
A
García-Pérez
C
C
Milán
R
R
Martínez
F
F
D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D001081:Apyrase / Q000037:antagonists & inhibitors; D001665:Binding Sites; D005260:Female; D006801:Humans; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D010920:Placenta / Q000378:metabolism* / Q000648:ultrastructure; D011247:Pregnancy; D011374:Progesterone / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D011506:Proteins / Q000378:metabolism
D011506:Proteins; D011374:Progesterone; C010959:5'-(4-fluorosulfonylbenzoyl)adenosine; D001081:Apyrase; D000241:Adenosine