title: Dermal fibroblasts respond to mechanical conditioning in a strain profile dependent manner.
abstract: Fibroblasts within tissues are exposed to a dynamic mechanical environment, which influences the structural integrity of both healthy and healing soft tissues. Various systems have been proposed to subject such cells to mechanical stimulation in culture. However the diverse nature of the studies, in terms of the strain profiles and the cell types, makes direct comparisons almost impossible. The present study addresses this issue by examining the metabolic response of two cell types subjected to three well defined strain profiles.A young fibroblast cell population, represented by HuFFs, showed both greater cell proliferation and collagen production than adult dermal fibroblasts under unstrained conditions. The three strain profiles produced differing effects on both cell types. Uniaxial strains enhanced [(3)H]-thymidine incorporation for both cell types, whilst biaxial strains either inhibited or had no effect on its incorporation. In contrast, [(3)H]-proline incorporation was inhibited under biaxial and uniaxial strains for the adult fibroblasts, whilst the HuFF cells showed a small increase in proline incorporation under non-uniform and uniaxial strains.
authors.affiliation: IRC in Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary, University of London, UK.
authors.forename: C C
authors.initials: CC
authors.lastname: Berry
authors.forename: C
authors.initials: C
authors.lastname: Cacou
authors.forename: D A
authors.initials: DA
authors.lastname: Lee
authors.forename: D L
authors.initials: DL
authors.lastname: Bader
authors.forename: J C
authors.initials: JC
authors.lastname: Shelton
mesh_terms: D000328:Adult; D000375:Aging / Q000502:physiology; D018929:Cell Culture Techniques; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D048429:Cell Size / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D003094:Collagen / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D010812:Physical Stimulation / Q000379:methods; D011392:Proline / Q000378:metabolism; D012867:Skin / Q000166:cytology*; D013314:Stress, Mechanical; D013936:Thymidine / Q000378:metabolism
chemical_list: D003094:Collagen; D011392:Proline; D013936:Thymidine