Aqueous humor levels of topically applied levofloxacin in human eyes.
OBJECTIVE
To evaluate transcorneal penetration of topically applied 0.5% levofloxacin into the aqueous humor in human eyes.


METHODS
Twenty cataract patients (14 females, 6 males) received 3 drops of 0.5% levofloxacin at 15 min intervals from 90 minutes before the surgery. At the beginning of the cataract surgery, 50 microL of aqueous humor was aspirated from the anterior chamber and stored at -80 degrees C until analysis. The drug concentration of the samples was analyzed using high-performance liquid chromatography.


RESULTS
A mean aqueous humor level of levofloxacin was 1.00 +/- 0.48 microg/mL (mean +/- standard deviation, n = 20), ranging from 0.30 microg/mL to 2.32 microg/mL.


CONCLUSIONS
The mean concentration of levofloxacin in the aqueous humor was higher than the MIC(90) values against some common pathogens of postoperative endophthalmitis, although a great degree of interpatient variability was present.
Yamada
Masakazu
M
Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan. yamadam@sc.itc.keio.ac.jp
Mochizuki
Hiroshi
H
Yamada
Kyoko
K
Kawai
Masataka
M
Mashima
Yukihiko
Y
D000287:Administration, Topical; D000368:Aged; D000369:Aged, 80 and over; D000890:Anti-Infective Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D001082:Aqueous Humor / Q000378:metabolism*; D005260:Female; D006801:Humans; D007158:Immunologic Techniques; D064704:Levofloxacin; D008297:Male; D008875:Middle Aged; D015242:Ofloxacin / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D009994:Osmolar Concentration
D000890:Anti-Infective Agents; D064704:Levofloxacin; D015242:Ofloxacin