Activation of peroxisome proliferator-activated receptor (PPAR)-gamma by 15S-hydroxyeicosatrienoic acid parallels growth suppression of androgen-dependent prostatic adenocarcinoma cells.
Although dietary gamma-linolenic acid (GLA) and its 15-lipoxygenase metabolite, 15S-hydroxyeicosatrienoic acid (15S-HETrE), have been reported to exert antiproliferative activities in other systems, their role in prostatic carcinogenesis is unknown. To evolve a possible mechanism for the suppressive effect on growth of prostatic cells, we incubated GLA and 15S-HETrE with androgen-dependent prostatic adenocarcinoma cells. 15S-HETrE but not GLA markedly inhibited [(3)H]thymidine uptake in parallel with the upregulation of peroxisome proliferator-activated receptor-gamma expression (a growth modulating nuclear receptor). The data, taken together, suggest that dietary GLA via its in vivo metabolite 15S-HETrE could serve as an endogenous adjunct to attenuate prostatic tumorigenesis.
Pham
Hung
H
Department of Dermatology, School of Medicine, University of California--Davis, Davis, CA 95616, USA.
Banerjee
Tinku
T
Nalbandian
Gregory M
GM
Ziboh
Vincent A
VA
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004537:Eicosanoic Acids / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D009376:Neoplasms, Hormone-Dependent / Q000378:metabolism* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis*; D014157:Transcription Factors / Q000096:biosynthesis*; D017965:gamma-Linolenic Acid / Q000494:pharmacology
D004537:Eicosanoic Acids; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D013196:Dihydrotestosterone; C073049:15-hydroxyeicosanoic acid; D017965:gamma-Linolenic Acid