title: Molecular diagnostic procedures for production of pathogen-free propagation material.
abstract: Production of disease-free propagation material is a major means of controlling most bacterial diseases of plants, particularly when neither resistant clones nor effective chemical treatments are available. For this purpose sensitive, specific and rapid detection methods are required. The advent of molecular biology and, in particular, the polymerase chain reaction (PCR) has opened new ways for the characterization and identification of plant pathogens and the development of disease-management strategies. PCR-based detection methods rely on the development of primers for the specific detection of the pathogen. The use of pathogenicity genes as targets for primer design is the preferred procedure for obtaining specific primers but other procedures may also be useful for this purpose. In the present review we describe four examples of procedures for detecting four important bacterial pathogens in Israel: Erwinia herbicola pv gypsophilae in gypsophila, Xanthomonas campestris pv pelargonii in geranium, Agrobacterium tumefaciens in asters and roses, and Xanthomonas campestris pv campestris in crucifers. Procedures for constructing specific PCR primers for each bacterium are illustrated and discussed as well as the combination of PCR with other methods.
authors.affiliation: Department of Plant Pathology, ARO, Volcani Center, Bet-Dagan, 50250, Israel. shulam@volcani.agri.gov.il
authors.forename: Shulamit
authors.initials: S
authors.lastname: Manulis
authors.forename: Laura
authors.initials: L
authors.lastname: Chalupowicz
authors.forename: Orit
authors.initials: O
authors.lastname: Dror
authors.forename: Frida
authors.initials: F
authors.lastname: Kleitman
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity; D031173:Aster Plant / Q000382:microbiology; D001419:Bacteria / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity; D019607:Brassicaceae / Q000382:microbiology; D029748:Caryophyllaceae / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D004885:Erwinia / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity; D031315:Geranium / Q000382:microbiology; D019684:Magnoliopsida / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D019105:Random Amplified Polymorphic DNA Technique; D027826:Rosa / Q000382:microbiology; D013047:Specific Pathogen-Free Organisms; D016959:Xanthomonas campestris / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity
chemical_list: D004269:DNA, Bacterial