Renal cell carcinoma: handling and treatment.
The quality of samples and of pre-analytical steps are crucial in all biological tests, this is dramatically true in proteomics analysis. In renal cell carcinoma preparation for two-dimensional gel electrophoresis the time elapsed between sample collection and treatment, and the heterogeneity of tissues are considered in order to obtain high quality and reproducibility of spots. The mechanical dissection and cell separation by magnetic beads coated with anti-Ber and EP4 antibodies to minimize the contamination of nonepithelial cells are described.
Sarto
Cecilia
C
University Department of Laboratory Medicine-Desio Hospital, Desio, Milan, Italy. sarto@uds.unimib.it
Valsecchi
Cristina
C
Mocarelli
Paolo
P
D014408:Biomarkers, Tumor / Q000032:analysis; D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D018189:Immunomagnetic Separation; D009363:Neoplasm Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins