title: Performance of a new chromogenic oxacillin resistance screen medium (Oxoid) in the detection and presumptive identification of methicillin-resistant Staphylococcus aureus.
abstract: A new chromogenic Oxacillin Resistance Screen Agar (ORSA; Oxoid) for the presumptive detection of methicillin-resistant Staphylococcus aureus (MRSA) was compared to a phenyl-mannitol-salt-oxacillin medium (MS-Oxa), blood agar and brain heart-infusion (BHI) on 579 clinical specimens. After 24 h [48h] sensitivity and specificity for ORSA vs. MS-Oxa were 50.8% [68.2%] vs. 53.8% [65.7%] and 95.6% [94.5%] vs. 92.7% [91.8], respectively. Within 24 h, ORSA and MS-Oxa identified 51% and 54% MRSA. It is not feasible to omit BHI in MRSA-screening protocols since 33% MRSA grew in BHI only.
authors.affiliation: Division of Clinical Microbiology, Hygiene-Institute of the University of Vienna, Vienna, Austria. petra.apfalter@akh-wien.ac.at
authors.forename: Petra
authors.initials: P
authors.lastname: Apfalter
authors.forename: Ojan
authors.initials: O
authors.lastname: Assadian
authors.forename: Andrea
authors.initials: A
authors.lastname: Kalczyk
authors.forename: Verena
authors.initials: V
authors.lastname: Lindenmann
authors.forename: Athanasios
authors.initials: A
authors.lastname: Makristathis
authors.forename: Stefan
authors.initials: S
authors.lastname: Mustafa
authors.forename: Manfred
authors.initials: M
authors.lastname: Rotter
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Hirschl
mesh_terms: D017077:Culture Media, Conditioned; D005260:Female; D006801:Humans; D008297:Male; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D010068:Oxacillin / Q000494:pharmacology*; D011237:Predictive Value of Tests; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification*
chemical_list: D017077:Culture Media, Conditioned; D010068:Oxacillin