title: Purification of (+)-dihydromyricetin from leaves extract of Ampelopsis grossedentata using high-speed countercurrent chromatograph with scale-up triple columns.
abstract: Purification of (+)-dihydromyricetin from an extract (16 g) of leaves of Ampelopsis grossedentata was performed using a preparative triple-column countercurrent chromatograph. With a solvent system composed of n-hexane-ethyl acetate-methanol-water (1:3:2:4, v/v) 11.3 g of (+)-dihydromyricetin was obtained at a high purity of over 99% by HPLC at 254 nm in 9 h.
authors.affiliation: Institute of Food and Biological Engineering, Hangzhou University of Commerce, China.
authors.forename: Qizhen
authors.initials: Q
authors.lastname: Du
authors.forename: Weijian
authors.initials: W
authors.lastname: Cai
authors.forename: Ming
authors.initials: M
authors.lastname: Xia
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D032421:Ampelopsis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000295:instrumentation* / Q000379:methods; D005419:Flavonoids / Q000302:isolation & purification*; D044948:Flavonols; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D044948:Flavonols; D010936:Plant Extracts; C472036:dihydromyricetin