Whole blood cryopreservation in epidemiological studies.
Standardized and cost-effective biological sample collection, processing, and storage procedures are needed in large-scale epidemiological studies to provide material for testing a broad range of etiological hypotheses. One component of sample collection in the Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial involves shipment of blood in acid-citrate-dextrose anticoagulant to a central processing laboratory, where 10% DMSO is added, and whole blood aliquots are cryopreserved. A single technician is able to routinely process 50-60 samples/day. Tests conducted to evaluate potential uses of cryopreserved whole blood showed successful EBV transformation (>90%, up to 20 months of storage). In addition, lymphocytes maintained good viability and stable T-cell:B-cell ratios, and T cells maintained the capacity to proliferate in response to solid phase anti-CD3/CD28 plus interleukin 2. Whole blood cryopreservation is a cost-effective approach to large-scale storage of viable cells in epidemiological studies.
Hayes
Richard B
RB
Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, Maryland 20892, USA. hayesr@mail.nih.gov
Smith
Craig O
CO
Huang
Wen-Yi
WY
Read
Yvonne
Y
Kopp
William C
WC
D000368:Aged; D001402:B-Lymphocytes / Q000502:physiology; D015415:Biomarkers / Q000097:blood; D001769:Blood; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002471:Cell Transformation, Neoplastic; D015179:Colorectal Neoplasms / Q000097:blood / Q000175:diagnosis; D003362:Cost-Benefit Analysis; D015925:Cryopreservation / Q000379:methods; D016021:Epidemiologic Studies; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis; D008213:Lymphocyte Activation / Q000502:physiology; D016246:Lymphocytes, Tumor-Infiltrating / Q000502:physiology; D008297:Male; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood / Q000175:diagnosis; D010949:Plasma / Q000737:chemistry / Q000166:cytology; D011471:Prostatic Neoplasms / Q000097:blood / Q000175:diagnosis; D012720:Severity of Illness Index; D013601:T-Lymphocytes / Q000502:physiology; D013997:Time Factors
D015415:Biomarkers