title: The canine minute virus (minute virus of canines) is a distinct parvovirus that is most similar to bovine parvovirus.
abstract: We characterized the genome and proteins of the canine minute virus (the minute virus of canines (MVC)). The genome sequence showed MVC to be an autonomous parvovirus encoding a large nonstructural protein 1 gene, a smaller nonstructural protein, and overlapping VP1 and VP2 protein genes. The virus was most closely related to bovine parvovirus (BPV), with which it was 43% identical at the DNA sequence level, while the NS1 and VP1 proteins were 33.6 and 41.4% identical to those of BPV, respectively. Spliced messages of the NS1 gene transcripts were detected by RT-PCR. VP1 and VP2 proteins were detected in purified capsids, as were modified versions of each protein, and VP3 was also found in full capsids.
authors.affiliation: James A. Baker Institute and Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Schwartz
authors.forename: Bryan
authors.initials: B
authors.lastname: Green
authors.forename: Leland E
authors.initials: LE
authors.lastname: Carmichael
authors.forename: Colin R
authors.initials: CR
authors.lastname: Parrish
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000235:genetics*; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D004285:Dogs / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017992:Parvovirus / Q000145:classification* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; C106202:NS1 protein, parvovirus; D017361:Viral Nonstructural Proteins