Kinetics of 1,1,1-trichloroethane transformation by iron sulfide and a methanogenic consortium.
To evaluate the effect of potential interactions between methanogenic bacteria and iron sulfide minerals during transformation of 1,1,1-trichloroethane (1,1,1-TCA), we measured the kinetics of 1,1,1-TCA transformation by mackinawite (FeS(1 - x), but abbreviated as FeS) and a methanogenic consortium enriched on lactate (termed LEC). Results from batch kinetic experiments show that 1,1,1-TCA transformation by FeS and resting LEC can be described by second-order rate expressions, with rates depending on 1,1,1-TCA concentration (M), FeS surface area concentration (m2 L(-1)), and LEC concentration (as measured by mg L(-1) volatile suspended solids (VSS)). In reactors containing FeS alone, 1,1-dichloroethane (1,1-DCA) and 2-butyne were identified as products, but only accounted for 6% of the 1,1,1-TCA transformed. In reactors containing LEC alone, the only identified product was 1,1-DCA, which accounted for 46 +/- 8% of the 1,1,1-TCA transformed. Supernatant from LEC-alone reactors also transformed 1,1,1-TCA, suggesting that 1,1,1-TCA may be transformed by some non-cell component (such as a extracellular compound excreted by the organisms) that either reacts directly with 1,1,1-TCA or with the abiotic media to form a reactive species. Comparison of 1,1,1-TCA transformation rates from experiments with combinations of FeS (varying surface area concentrations) and LEC (varying VSS concentrations) to those with just FeS alone or LEC alone suggests some synergism occurs between the two reactive species. Observed enhancements took the form of faster 1,1,1-TCA transformation and faster 1,1-DCA appearance but less production of 1,1-DCA per unit of 1,1,1-TCA transformed. These observations suggest that the faster 1,1,1-TCA transformation in the combined systems (compared to the FeS-alone and LEC-alone experiments) is due to increased reactivity of both FeS and LEC, possibly due to production of soluble microbial products that make the FeS more reactive or less inhibition of LEC by 1,1,1-TCA due to FeS transformation of 1,1,1-TCA.
Gander
Jerry W
JW
Department of Civil and Environmental Engineering, The University of Iowa, Iowa City 52242, USA.
Parkin
Gene F
GF
Scherer
Michelle M
MM
D019605:Euryarchaeota / Q000502:physiology*; D005296:Ferrous Compounds / Q000737:chemistry*; D007700:Kinetics; D012995:Solubility; D012997:Solvents / Q000737:chemistry*; D014240:Trichloroethanes / Q000737:chemistry*; D014835:Volatilization; D014873:Water Pollutants; D018508:Water Purification
D005296:Ferrous Compounds; D012997:Solvents; D014240:Trichloroethanes; D014873:Water Pollutants; C024566:1,1,1-trichloroethane; C022597:ferrous sulfide