title: Neurotrophin-3 is required for appropriate establishment of thalamocortical connections.
abstract: In the vertebrate brain, the thalamus serves as a relay and integration station for diverse neuronal information en route from the periphery to the cortex. Formation of the thalamocortical tract occurs during pre- and postnatal development, with distinct thalamic nuclei projecting to specific cortical regions. The molecular forces that underlie the invasion by axons into specific cortical layers followed by activity-dependent maturation of synapses are poorly understood. We show that genetic ablation of neurotrophin-3 (NT-3) in the mouse neocortex results in reduction of a set of anatomically distinct axonal bundles projecting from thalamus through cortical white matter. These bundles include thalamocortical axons that normally establish connections with retrosplenial and visual cortex, sites of early postnatal NT-3 expression. These results implicate neurotrophins in the critical stage of precise thalamocortical connections.
authors.affiliation: Center for Developmental Biology and Kent Waldrep Foundation Center for Basic Research on Nerve Growth and Regeneration, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors.forename: Long
authors.initials: L
authors.lastname: Ma
authors.forename: Takayuki
authors.initials: T
authors.lastname: Harada
authors.forename: Chikako
authors.initials: C
authors.lastname: Harada
authors.forename: Mario
authors.initials: M
authors.lastname: Romero
authors.forename: Jean M
authors.initials: JM
authors.lastname: Hebert
authors.forename: Susan K
authors.initials: SK
authors.lastname: McConnell
authors.forename: Luis F
authors.initials: LF
authors.lastname: Parada
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002450:Cell Communication / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D002540:Cerebral Cortex / Q000002:abnormalities* / Q000166:cytology / Q000378:metabolism; D002633:Chemotaxis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D051858:Forkhead Transcription Factors; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D020439:Growth Cones / Q000378:metabolism* / Q000648:ultrastructure; D019426:Integrases / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation / Q000235:genetics; D009413:Nerve Fibers, Myelinated / Q000378:metabolism / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000235:genetics; D009434:Neural Pathways / Q000002:abnormalities* / Q000166:cytology / Q000378:metabolism; D020933:Neurotrophin 3 / Q000172:deficiency* / Q000235:genetics; D013788:Thalamus / Q000002:abnormalities* / Q000166:cytology / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics; D014786:Vision Disorders / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D014793:Visual Cortex / Q000002:abnormalities / Q000166:cytology / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D051858:Forkhead Transcription Factors; C489562:Foxd1 protein, mouse; D009419:Nerve Tissue Proteins; D020933:Neurotrophin 3; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases