[Effects of different frequency electromagnetic fields on the differentiation of midbrain neural stem cells].
Objective. To examine the effect of electromagnetic fields (EMF) (20 Hz, 8 mT; 5 Hz, 8 mT) on the neuron-orientated differentiation of neural stem cells (NSCs) from midbrains of new-bom rats. Method. Differentiated NSCs were exposed to EMF for 2 x 15 min per day lasting for I d, 5 d, or 10 d. The sham-exposure controls were correspondingly established. Cells were fixed and processed for immunofluorescent staining using the antibody against neuron-specific marker MAP2, then the percentage of MAP2+ cells was calculated. Result. The two EMFs promoted the differentiation of a neuronal fate in different ways. Both of them came into effect even after 1 d exposure. When cells exposed to the 20 Hz EMF, the percentage of neuron-orientated cells gradually increased with longer-term exposure and the most significant effect appeared in 10 d group while that happened in 5 d group under the condition of 5 Hz EMF. The effect contrasted horizontally, significant differences between the two EMFs were observed only at 10 d groups, 20 Hz EMF having more favorable effect than 5 Hz EMF. Conclusion. 20 Hz and 5 Hz EMF could promote the differentiation of midbrain NSCs to a neuronal phenotype in different ways, suggesting that the physical induction might be another strategy to manipulate the differentiation of NSCs.
Li
Yi
Y
The Second Military Medical University, Shanghai, China.
Zhao
Lun
L
Xing
Xuan
X
Lou
Shu-jie
SJ
He
Cheng
C
Lu
Chang-lin
CL
D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000502:physiology / Q000528:radiation effects*; D002478:Cells, Cultured / Q000528:radiation effects; D004574:Electromagnetic Fields; D008636:Mesencephalon / Q000166:cytology / Q000528:radiation effects*; D009474:Neurons / Q000502:physiology / Q000528:radiation effects*; D051381:Rats; D013234:Stem Cells / Q000502:physiology / Q000528:radiation effects*; D013997:Time Factors