Spatial characterisation of ryanodine-induced calcium release in mouse pancreatic acinar cells.
In pancreatic acinar cells, agonists evoke intracellular Ca(2+) transients which are initiated in the apical region of these polarized cells. There are contradictory experimental data concerning Ca(2+) release from ryanodine receptors (RyRs) in the apical region. In the present study, we have used low doses of ryanodine to open RyRs leading to the release of Ca(2+) from intracellular stores. Ryanodine causes Ca(2+) release that is initiated in the apical region of the cell but is dependent upon functional inositol 1,4,5-trisphosphate receptors (IP(3)Rs). These results suggests that co-ordinated release from co-localized RyRs and IP(3)Rs underlies the increased sensitivity of the apical region to initiation of intracellular Ca(2+) transients.
Ashby
Michael C
MC
Medical Research Council Secretory Control Research Group, The Physiological Laboratory, University of Liverpool, UK. ashby@liv.ac.uk
Petersen
Ole H
OH
Tepikin
Alexei V
AV
D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D053496:Inositol 1,4,5-Trisphosphate Receptors; D051379:Mice; D010179:Pancreas / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000187:drug effects / Q000378:metabolism; D012433:Ryanodine / Q000494:pharmacology*; D019837:Ryanodine Receptor Calcium Release Channel / Q000187:drug effects / Q000378:metabolism
D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018160:Receptors, Cytoplasmic and Nuclear; D019837:Ryanodine Receptor Calcium Release Channel; D012433:Ryanodine; D002110:Caffeine; D002118:Calcium