title: Lipids are co-eluted with immunoglobulins G during purification by recombinant streptococcal protein G affinity chromatography.
abstract: The efficiency of recombinant streptococcal protein G (rec-spG) affinity column chromatography in purifying immunoglobulins G (IgG) from lipids has been studied, with particular reference to IgG fractions from bronchoalveolar lavage (BAL) fluid and serum samples from different sources. It was found that the IgG fractions purified by rec-spG affinity column chromatography also contained cholesterol and phospholipids.
authors.affiliation: Laboratoire de Biogenèse Membranaire CNRS-UMR 5544, Université Victor Segalen, Bordeaux 2, France. me00157@cc.uoi.gr
authors.forename: Eirini
authors.initials: E
authors.lastname: Kitsiouli
authors.forename: Marilena E
authors.initials: ME
authors.lastname: Lekka
authors.forename: George
authors.initials: G
authors.lastname: Nakos
authors.forename: Claude
authors.initials: C
authors.lastname: Cassagne
authors.forename: Lilly
authors.initials: L
authors.lastname: Maneta-Peyret
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002784:Cholesterol / Q000097:blood; D002846:Chromatography, Affinity / Q000379:methods* / Q000592:standards; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification*; D008055:Lipids / Q000097:blood / Q000302:isolation & purification*; D010743:Phospholipids / Q000097:blood; D011817:Rabbits
chemical_list: D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D007074:Immunoglobulin G; D008055:Lipids; D010743:Phospholipids; D002784:Cholesterol