Competitive PCR for quantitation of a Cytophaga-Flexibacter-Bacteroides phylum bacterium associated with the Tuber borchii Vittad. mycelium.
An uncultured bacterium associated with the ectomycorrhizal fungus Tuber borchii Vittad. was identified as a novel member of the Cytophaga-Flexibacter-Bacteroides group. Utilizing a quantitative PCR targeting the 16S rRNA gene, we relatively quantified this bacterium in the host. The estimated number of bacteria was found to be approximately 10(6) cells per 30-day-old T. borchii mycelium culture. This represents the first molecular attempt to enumerate an uncultured bacterium associated with a mycorrhizal fungus.
Barbieri
Elena
E
Giorgio Fornaini Institute of Biochemistry, University of Urbino, Pesaro-Urbino, Italy.
Riccioni
Giulia
G
Pisano
Anna
A
Sisti
Davide
D
Zeppa
Sabrina
S
Agostini
Deborah
D
Stocchi
Vilberto
V
D001203:Ascomycota; D001439:Bacteroides / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D003589:Cytophaga / Q000235:genetics / Q000302:isolation & purification*; D005417:Flavobacterium / Q000235:genetics / Q000302:isolation & purification*; D018628:Gene Dosage; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D025282:Mycelium; D009876:Operon; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D012336:RNA, Ribosomal, 16S