Expression of protective antigen in transgenic plants: a step towards edible vaccine against anthrax.
Protective antigen (PA) is the most potent molecule for vaccination against anthrax. In the present study, we have successfully integrated protective antigen gene in nuclear genome of tobacco plants by Agrobacterium mediated leaf-disc transformation method. Expression of protective antigen gene was detected by immunoblot analysis using antisera raised against purified PA. A distinct band of approximately 83kDa lighted up in the protein extracted from transformed plants while there was no such band in untransformed plants. The plant expressed PA showed biological activity just like native PA, which was demonstrated by cytolytic assay on macrophage like cell lines with lethal factor. This study establishes for the first time expression of PA gene in a plant system and thus marks the first milestone towards developing edible vaccine against anthrax.
Aziz
Mohd Azhar
MA
Centre for Biotechnology, Jawaharlal Nehru University, 110067, New Delhi, India.
Singh
Samer
S
Anand Kumar
P
P
Bhatnagar
Rakesh
R
D000881:Anthrax / Q000276:immunology / Q000517:prevention & control*; D000942:Antigens, Bacterial / Q000276:immunology / Q000378:metabolism; D001408:Bacillus anthracis / Q000276:immunology*; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic; D022462:Vaccines, Edible / Q000235:genetics / Q000276:immunology
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D022462:Vaccines, Edible; C030325:anthrax toxin