title: Complementary antioxidant defense by cytoplasmic and mitochondrial peroxiredoxins in Leishmania infantum.
abstract: In Kinetoplastida 2-Cys peroxiredoxins are the ultimate members of unique enzymatic cascades for detoxification of peroxides, which are dependent on trypanothione, a small thiol specific to these organisms. Here we report on two distinct Leishmania infantum peroxiredoxins, LicTXNPx and LimTXNPx, that may be involved in such a pathway. LicTXNPx, found in the cytoplasm, is a typical 2-Cys peroxiredoxin encoded by LicTXNPx, a member of a multicopy gene family. LimTXNPx, encoded by a single copy gene, LimTXNPx, is confined to the mitochondrion and is unusual in possessing an Ile-Pro-Cys motif in the distal redox center, replacing the common peroxiredoxin Val-Cys-Pro sequence, apart from an N-terminal mitochondrial leader sequence. Based on sequence and subcellular localization, the peroxiredoxins of Kinetoplastida can be separated in two distinct subfamilies. As an approach to investigate the function of both peroxiredoxins in the cell, L. infantum promastigotes overexpressing LicTXNPx and LimTXNPx were assayed for their resistance to H(2)O(2) and tert-butyl hydroperoxide. The results show evidence that both enzymes are active as peroxidases in vivo and that they have complementary roles in parasite protection against oxidative stress.
authors.affiliation: Institute for Molecular and Cell Biology, Porto, Portugal.
authors.forename: Helena
authors.initials: H
authors.lastname: Castro
authors.forename: Carla
authors.initials: C
authors.lastname: Sousa
authors.forename: Marta
authors.initials: M
authors.lastname: Santos
authors.forename: Anabela
authors.initials: A
authors.lastname: Cordeiro-da-Silva
authors.forename: Leopold
authors.initials: L
authors.lastname: Flohé
authors.forename: Ana M
authors.initials: AM
authors.lastname: Tomás
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D015139:Blotting, Southern; D015153:Blotting, Western; D003360:Cosmids; D003593:Cytoplasm / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D018105:DNA, Kinetoplast / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals; D015723:Gene Library; D005822:Genetic Vectors; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018314:Leishmania infantum / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010544:Peroxidases / Q000378:metabolism*; D054464:Peroxiredoxins; D010641:Phenotype; D010802:Phylogeny; D015800:Protozoan Proteins; D012313:RNA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013438:Sulfhydryl Compounds; D014162:Transfection; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D018105:DNA, Kinetoplast; D005609:Free Radicals; D015800:Protozoan Proteins; D013438:Sulfhydryl Compounds; D012313:RNA; D004247:DNA; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D010544:Peroxidases; C110959:tryparedoxin peroxidase; D054464:Peroxiredoxins