title: Validation of a new fluorogenic real-time PCR assay for detection of CYP2C9 allelic variants and CYP2C9 allelic distribution in a German population.
abstract: OBJECTIVE
  The aim was to develop a fast detection method for the polymorphic alleles related to impaired CYP2C9-mediated metabolism.
  
  
  METHODS
  CYP2C9 genotypes were identified in 118 DNA samples using real-time PCR (LightCycler) followed by melting curve analysis. All samples were re-tested by validated PCR-RFLP methodology.
  
  
  RESULTS
  The concordance between the two methods was 100% for two variant alleles. The frequencies of CYP2C9*1 (wild type), CYP2C9*2 and CYP2C9*3 (with 95% confidence intervals) were 0.81(0.05), 0.14(0.04) and 0.05(0.03), respectively, and are similar to those observed in other Caucasian populations.
  
  
  CONCLUSIONS
  This assay is simple and rapid and may be used for CYP2C9-genotyping in a clinical setting.
authors.affiliation: Pharmazentrum Frankfurt, Institut für Klinische Pharmakologie, Klinikum der J.W. Goethe-Universität, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Burian
authors.forename: Sabine
authors.initials: S
authors.lastname: Grösch
authors.forename: Irmgard
authors.initials: I
authors.lastname: Tegeder
authors.forename: Gerd
authors.initials: G
authors.lastname: Geisslinger
mesh_terms: D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D020137:Base Pair Mismatch; D065729:Cytochrome P-450 CYP2C9; D005453:Fluorescence; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D005858:Germany / Q000208:ethnology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length
chemical_list: C450260:CYP2C9 protein, human; D065729:Cytochrome P-450 CYP2C9; D001189:Aryl Hydrocarbon Hydroxylases