High-resolution genetic mapping with ordered arrays of Saccharomyces cerevisiae deletion mutants.
We present a method for high-resolution genetic mapping that takes advantage of the ordered set of viable gene deletion mutants, which form a set of colinear markers covering almost every centimorgan of the Saccharomyces cerevisiae genome, and of the synthetic genetic array (SGA) system, which automates the construction of double mutants formed by mating and meiotic recombination. The Cbk1 kinase signaling pathway, which consists minimally of CBK1, MOB2, KIC1, HYM1, and TAO3 (PAG1), controls polarized morphogenesis and activation of the Ace2 transcription factor. Deletion mutations in the Cbk1 pathway genes are tolerated differently by common laboratory strains of S. cerevisiae, being viable in the W303 background but dead in the S288C background. Genetic analysis indicated that the lethality of Cbk1 pathway deletions in the S288C background was suppressed by a single allele specific to the W303 background. SGA mapping (SGAM) was used to locate this W303-specific suppressor to the SSD1 locus, which contains a known polymorphism that appears to compromise SSD1 function. This procedure should map any mutation, dominant or recessive, whose phenotype is epistatic to wild type, that is, a phenotype that can be scored from a mixed population of cells obtained by germination of both mutant and wild-type spores. In principle, SGAM should be applicable to the analysis of multigenic traits. Large-scale construction of ordered mutations in other model organisms would broaden the application of this approach.
Jorgensen
Paul
P
Department of Medical Genetics and Microbiology, University of Toronto, Ontario M5S 1A8, Canada.
Nelson
Bryce
B
Robinson
Mark D
MD
Chen
Yiqun
Y
Andrews
Brenda
B
Tyers
Mike
M
Boone
Charles
C
D048868:Adaptor Proteins, Signal Transducing; D002135:Calcium-Binding Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics; D002868:Chromosomal Proteins, Non-Histone; D002874:Chromosome Mapping; D005656:Fungal Proteins / Q000235:genetics; D005804:Genes, Lethal; D016153:Genes, Suppressor; D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010750:Phosphoproteins / Q000235:genetics; D017346:Protein Serine-Threonine Kinases; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D017384:Sequence Deletion
D048868:Adaptor Proteins, Signal Transducing; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; C444039:MOB2 protein, S cerevisiae; D008869:Microtubule-Associated Proteins; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; C456842:TAO3 protein, S cerevisiae; C418662:TBCD protein, human; C056608:caltractin; C423362:CBK1 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases