title: Tight binding of influenza virus hemagglutinin to its receptor interferes with fusion pore dilation.
abstract: Deletion of oligosaccharide side chains near the receptor binding site of influenza virus A/USSR/90/77 (H1N1) hemagglutinin (HA) enhanced the binding of HA to erythrocyte receptors, as was also observed with A/FPV/Rostock/34 (H7N1). Correlated with the enhancement of binding activity, the cell fusion activity of HA was reduced. A mutant HA in which three oligosaccharide side chains were deleted showed the highest level of binding and the lowest level of fusion among the HAs tested. The cell fusion activity of the oligosaccharide deletion mutant of HA, however, was drastically elevated when the binding activity was reduced by deletion of four amino acids adjacent to the receptor binding site. Thus, a reciprocal relationship was observed between the receptor binding and the cell fusion activities of H1/USSR HA. No difference was observed, however, in lipid mixing activity, so-called hemifusion, between wild-type (WT) and oligosaccharide deletion mutant HAs. Soluble dye transfer testing showed that even the HA with the lowest cell fusion activity was able to form fusion pores through which a small molecule such as calcein could pass. However, electron microscopic studies revealed that a large molecule such as hemoglobin hardly passed through the fusion pores formed by the mutant HA, whereas hemoglobin did efficiently pass through those formed by the WT HA. These results suggested that interference in the process of dilation of fusion pores occurs when the binding of HA to the receptor is too tight. Since the viral nucleocapsid is far larger than hemoglobin, appropriate receptor binding affinity is important for virus entry.
authors.affiliation: Department of Microbiology, Kawasaki Medical School, Kurashiki 701-0192, Japan. mohuchi@med.kawasaki-m.ac.jp
authors.forename: Masanobu
authors.initials: M
authors.lastname: Ohuchi
authors.forename: Reiko
authors.initials: R
authors.lastname: Ohuchi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Sakai
authors.forename: Akira
authors.initials: A
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D001665:Binding Sites; D004912:Erythrocytes / Q000378:metabolism; D006168:Guinea Pigs; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000378:metabolism*; D008561:Membrane Fusion / Q000502:physiology*
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus