Low levels of co-receptor CCR5 are sufficient to permit HIV envelope-mediated fusion with resting CD4 T cells.
The susceptibility of phenotypically CCR5-negative resting CD4 T cells for membrane fusion with a CCR5-specific HIV-1 envelope was analysed using a novel sensitive fusion assay. A very low overall density of CCR5 on T cells expressing high levels of CD4 was shown to be sufficient for HIV envelope-mediated membrane fusion. These findings are relevant to the understanding of how HIV-1 R5 strains enter and replicate in resting CD4 T cells in vivo.
Chanel
Chantal
C
Unite de Immunologie Virale, Institut Pasteur, 75015 Paris, France.
Staropoli
Isabelle
I
Baleux
Francoise
F
Amara
Ali
A
Valenzuela-Fernandez
Agustin
A
Virelizier
Jean-Louis
JL
Arenzana-Seisdedos
Fernando
F
Altmeyer
Ralf
R
D000911:Antibodies, Monoclonal / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism / Q000821:virology*; D015699:HIV Envelope Protein gp120 / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D008213:Lymphocyte Activation; D019713:Receptors, CCR5 / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015699:HIV Envelope Protein gp120; D019713:Receptors, CCR5; D019718:Receptors, CXCR4