Ginsenosides inhibit EGF-induced proliferation of renal proximal tubule cells via decrease of c-fos and c-jun gene expression in vitro.
Recent epidemiological studies have demonstrated that ginseng intake is associated with a reduced risk for environmentally related cancers. However, the effects of ginsenosides on the proliferation of renal proximal tubule cells have not yet elucidated. This study investigated the effect of total ginsenosides, protopanaxatriol (PT) saponin, and protopanaxadiol (PD) saponin fraction on epidermal growth factor (EGF)-induced renal cell proliferation and, furthermore, c-fos and c-jun gene expression. In the present study, total ginsenosides (10 -6 g/ml) completely blocked EGF-induced DNA synthesis and cell growth. In contrast, the PT and PD fractions partially blocked it. In addition, the EGF-induced increase of c-fos and c-jun gene expression was completely blocked by total ginsenosides and partially by PT and PD saponins. In conclusion, ginsenosides, in part, inhibit EGF-induced cell proliferation via decrease of c-fos and c-jun gene expression in primary cultured rabbit renal proximal tubular cells (PTCs). Abbreviations. EGF:epidermal growth factor PD:protopanaxadiol PT:protopanaxatriol PTCs:primary cultured renal proximal tubule cells
Han
Ho Jae
HJ
Department of Veterinary Physiology, College of Veterinary Medicine, Hormone Research Center, Chonnam National University, Kwangju 500-757, Korea. hjhan@chonnam.ac.kr
Yoon
Byeong Cheol
BC
Lee
Sang Hern
SH
Park
Soo Hyun
SH
Park
Ji Yeong
JY
Oh
Young Joon
YJ
Lee
Yun Jung
YJ
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor; D015972:Gene Expression Regulation, Neoplastic; D016762:Genes, fos / Q000235:genetics; D016758:Genes, jun / Q000235:genetics; D036145:Ginsenosides / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D007680:Kidney Neoplasms / Q000517:prevention & control; D007687:Kidney Tubules, Proximal / Q000166:cytology* / Q000187:drug effects*; D008297:Male; D005894:Panax; D008517:Phytotherapy; D018517:Plant Roots; D011817:Rabbits; D012502:Sapogenins / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D013936:Thymidine / Q000378:metabolism; D014315:Triterpenes / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
D036145:Ginsenosides; D012502:Sapogenins; D014315:Triterpenes; C081552:protopanaxatriol; D004815:Epidermal Growth Factor; D013936:Thymidine