title: Shear stress modulates tumour cell adhesion to the endothelium.
abstract: The adhesion of breast adenocarcinoma cells (MDA-MB-231) to human umbilical vein endothelial cells (HUVEC) was studied in whole blood and under varying flow conditions. This study was done on HUVEC either kept under static conditions or pre-conditioned in flow for 2 hours at a shear stress of 5 or 13 dyn/cm(2). Coverslips coated by HUVEC were placed in a parallel plate perfusion chamber and perfused at a shear rate of 300 or 1500 sec(-1) with heparin-anticoagulated blood containing 111In labelled MDA-MB-231 cells. We report here the optimal conditions for studying the adhesion of MDA-MB-231 to endothelial cells under shear constraints corresponding to those observed into small and medium sized arteries.
authors.affiliation: U553 INSERM, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75475 Paris Cedex 10, France.
authors.forename: N
authors.initials: N
authors.lastname: Gomes
authors.forename: M
authors.initials: M
authors.lastname: Berard
authors.forename: J
authors.initials: J
authors.lastname: Vassy
authors.forename: N
authors.initials: N
authors.lastname: Peyri
authors.forename: C
authors.initials: C
authors.lastname: Legrand
authors.forename: F
authors.initials: F
authors.lastname: Fauvel-Lafeve
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D001943:Breast Neoplasms / Q000473:pathology*; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000473:pathology*; D005260:Female; D006801:Humans; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D013314:Stress, Mechanical; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins / Q000473:pathology