title: Asynchronous replication of alleles in genomes carrying a microdeletion.
abstract: BACKGROUND
  While most allelic pairs of DNA replicate synchronously during the S phase of the cell cycle, some genes normally replicate asynchronously, i.e., genes on the X chromosome and imprinted genes. The replication control mechanism is unknown but was shown to be impaired in malignancies and chromosomal trisomies where replication pattern becomes asynchronous.
  
  
  OBJECTIVE
  To determine the level of asynchronization in replication timing of cells from patients with microdeleted genomes.
  
  
  METHODS
  We applied monocolor fluorescent in situ hybridization with different probes on leukocytes from microdeleted genomes.
  
  
  RESULTS
  All samples derived from the microdeleted genomes showed significantly higher levels of an asynchronized pattern compared to normal individuals.
  
  
  CONCLUSIONS
  Even a "small" genetic imbalance (microdeletion) can interfere with gene replication and cell cycle progression, as previously shown in full trisomies.
authors.affiliation: Genetic Institute, Meir General Hospital, Kfar Saba, Israel. alizaamiel@hotmail.com
authors.forename: Aliza
authors.initials: A
authors.lastname: Amiel
authors.forename: Orit
authors.initials: O
authors.lastname: Reish
authors.forename: Elena
authors.initials: E
authors.lastname: Gaber
authors.forename: Ronit
authors.initials: R
authors.lastname: Masterman
authors.forename: Tally
authors.initials: T
authors.lastname: Tohami
authors.forename: Moshe D
authors.initials: MD
authors.lastname: Fejgin
mesh_terms: D022981:Allelic Imbalance / Q000235:genetics*; D002904:Chromosomes, Human, 21-22 and Y / Q000235:genetics; D004261:DNA Replication / Q000235:genetics*; D017353:Gene Deletion; D016259:Genes, myc / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007962:Leukocytes / Q000502:physiology; D015336:Molecular Probe Techniques; D016160:Retinoblastoma Protein / Q000235:genetics; D016196:S Phase / Q000235:genetics
chemical_list: D016160:Retinoblastoma Protein