Enumeration of Legionella CFU by colony hybridization using specific DNA probes.
Oligonucleotide probes and colony hybridization (CH) were applied to enumerate organisms of the genus Legionella in cooling tower water. The CH counts indicated almost the same results as CFU counts in cultivated samples derived from the water. It was concluded that it is possible to substitute the CH procedure for the conventional one.
Satoh
Wakako
W
Technology and Development Laboratory, Kurita Water Industries Ltd., Kanagawa 243-0124, Japan. wakako.Satoh@kurita.co.jp
Nakata
Mayumi
M
Yamamoto
Hiroyuki
H
Ezaki
Takayuki
T
Hiramatsu
Keiichi
K
D015169:Colony Count, Microbial / Q000379:methods*; D007875:Legionella / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000235:genetics*; D012680:Sensitivity and Specificity
D015345:Oligonucleotide Probes