Human atherosclerotic plaques express DC-SIGN, a novel protein found on dendritic cells and macrophages.
The association of autoimmune phenomena with atherosclerosis suggests that plaques may contain specialized antigen-presenting cells, dendritic cells (DCs). DC-SIGN is a C-type lectin expressed by DCs. This study assessed whether human atherosclerotic plaques expressed DC-SIGN and several other macrophage/DC markers. Plaques from human coronary and carotid arteries and aorta contained DC-SIGN-immunoreactive cells. Double-labelling showed co-expression of DC-SIGN and macrophage/DC lineage markers CD14, CD68, HLA-DR, and S100. There was no immunoreactivity for the DC activation markers CD83 or CMRF-44. Since DC-SIGN mediates adhesion to T-lymphocytes and endocytosis, its expression in atherosclerotic plaques may have functional implications. Activated DCs migrate quickly from areas of inflammation to regional lymph nodes, possibly explaining the paucity of activated DCs in atherosclerotic plaques. In conclusion, this study has shown that DC-SIGN is expressed in atherosclerosis.
Soilleux
E J
EJ
Medical Research Council Cancer Centre Unit, Hutchinson/MRC Research Centre, Cambridge, UK.
Morris
L S
LS
Trowsdale
J
J
Coleman
N
N
Boyle
J J
JJ
D001012:Aorta, Abdominal / Q000378:metabolism; D001018:Aortic Diseases / Q000378:metabolism / Q000473:pathology; D001161:Arteriosclerosis / Q000378:metabolism* / Q000473:pathology; D015415:Biomarkers / Q000032:analysis; D002340:Carotid Artery Diseases / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003324:Coronary Artery Disease / Q000378:metabolism / Q000473:pathology; D003713:Dendritic Cells / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D037181:Lectins, C-Type / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D015415:Biomarkers; D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin