Immunofluorescence detection of Escherichia coli in seawater: a comparison of various commercial antisera.
Through a microscopical method, relying on the interaction between fluorescent antibodies and target antigen, it is possible to detect and enumerate Escherichia coli in seawaters. Various commercial monoclonal and polyclonal antisera have been tested in an indirect immunofluorescence (IIF) assay developed for microbiological monitoring of coastal waters. Prior to use, they have been titrated and screened for cross-reactions with a collection of clinical and environmental isolates. A comparison among counts obtained on field samples showed higher performance for microscopical than for plate methods, due to the ability of all antisera to label target cells specifically, regardless of their viability. Because of their different specificities, polyclonal antisera yielded better quantitative results than monoclonal antisera. The study further suggested the usefulness of the immunofluorescence assay as a rapid alternative analytical tool for the specific detection of bacterial pathogens in aquatic environments.
Caruso
G
G
Istituto Sperimentale Talassografico, Istituto Sperimentale Talassografico CNR, Spianata S. Raineri 86, 98122 Messina, Italy. caruso@ist.me.cnr.it
Crisafi
E
E
Mancuso
M
M
D015169:Colony Count, Microbial / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D012623:Seawater / Q000382:microbiology*; D012680:Sensitivity and Specificity