Molecular characterization of foot-and-mouth disease virus O/SKR/2000.
Molecular cloning and sequencing of the genome of foot-and-mouth disease virus (FMDV) O/SKR/2000, one of PanAsia strain, were performed from FMDV infected cattle. From the poly (C) tract of the 5' nontranslated region (NTR) to the 3' NTR including 14 base pairs (bp) of poly (A) tail, 7813 bp sequences comprising approximately 95% of the whole genome were obtained by reverse transcription polymerase reaction (RT-PCR). The deduced amino acid sequences of the structural and nonstructural proteins (NSP) of the O/SKR/2000 virus were analyzed for the sequence similarity among type O strains. Comparison between FMDV O/SKR/2000 and other strains indicates that overall the number of amino acids appears to be conserved without any deletion in either NSP or capsid proteins, thus, suggesting that O/SKR/2000 evolved with minor difference from preexisting strains.
Kweon
Chang Hee
CH
Virology Research Division, National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, Anyang, South Korea. kweonch@mail.nvrqs.go.kr
Ko
Young Joon
YJ
Kim
Won Il
WI
Kwon
Byung Joon
BJ
Hyun
Bang Hun
BH
Sohn
Hyun Joo
HJ
Choi
Kang Seuk
KS
Shin
Jin Ho
JH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D003001:Cloning, Molecular; D005536:Foot-and-Mouth Disease / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D014764:Viral Proteins