title: Evidence for antibody-catalyzed ozone formation in bacterial killing and inflammation.
abstract: Recently, we showed that antibodies catalyze the generation of hydrogen peroxide (H2O2) from singlet molecular oxygen (1O2*) and water. Here, we show that this process can lead to efficient killing of bacteria, regardless of the antigen specificity of the antibody. H2O2 production by antibodies alone was found to be not sufficient for bacterial killing. Our studies suggested that the antibody-catalyzed water-oxidation pathway produced an additional molecular species with a chemical signature similar to that of ozone. This species is also generated during the oxidative burst of activated human neutrophils and during inflammation. These observations suggest that alternative pathways may exist for biological killing of bacteria that are mediated by potent oxidants previously unknown to biology.
authors.affiliation: Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Wentworth
authors.forename: Jonathan E
authors.initials: JE
authors.lastname: McDunn
authors.forename: Anita D
authors.initials: AD
authors.lastname: Wentworth
authors.forename: Cindy
authors.initials: C
authors.lastname: Takeuchi
authors.forename: Jorge
authors.initials: J
authors.lastname: Nieva
authors.forename: Teresa
authors.initials: T
authors.lastname: Jones
authors.forename: Cristina
authors.initials: C
authors.lastname: Bautista
authors.forename: Julie M
authors.initials: JM
authors.lastname: Ruedi
authors.forename: Abel
authors.initials: A
authors.lastname: Gutierrez
authors.forename: Kim D
authors.initials: KD
authors.lastname: Janda
authors.forename: Bernard M
authors.initials: BM
authors.lastname: Babior
authors.forename: Albert
authors.initials: A
authors.lastname: Eschenmoser
authors.forename: Richard A
authors.initials: RA
authors.lastname: Lerner
mesh_terms: D000818:Animals; D017151:Antibodies, Catalytic / Q000276:immunology / Q000378:metabolism*; D001183:Arthus Reaction / Q000276:immunology* / Q000378:metabolism; D001770:Blood Bactericidal Activity; D002374:Catalase / Q000378:metabolism; D002384:Catalysis; D004926:Escherichia coli / Q000276:immunology*; D006415:Hematoporphyrins / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007203:Indigo Carmine / Q000378:metabolism; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D018375:Neutrophil Activation; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*; D010084:Oxidation-Reduction; D010126:Ozone / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D016897:Respiratory Burst; D026082:Singlet Oxygen / Q000378:metabolism; D014466:Ultraviolet Rays; D014867:Water / Q000378:metabolism
chemical_list: D017151:Antibodies, Catalytic; D006415:Hematoporphyrins; D014867:Water; D026082:Singlet Oxygen; D010126:Ozone; D006861:Hydrogen Peroxide; D007203:Indigo Carmine; D002374:Catalase