Carbazole alkaloids as new cell cycle inhibitor and apoptosis inducers from Clausena dunniana Levl.
Carbazole alkaloids, 3-methylcarbazole (1), murrayafoline A (2), girinimbine (3), mahanimbine (4) and bicyclomahanimbine (5), were isolated for the first time from Clausena dunniana Levl. by bioassay-guided separation procedure and were identified by spectroscopic methods. Compounds 1-5 showed growth inhibitory activity (1, IC50 25 microg/ml) on human fibrosarcoma HT-1080 cells and cell cycle M-phase inhibitory (2, MIC 0.78 microg/ml) and apoptosis inducing (2, MIC 1.56 microg/ml; 3, MIC 25 microg/ml; 4, MIC 20 microg/ml; 5, MIC 30 microg/ml) activities on mouse tsFT210 cells, respectively, and 2-5 provided the first examples of carbazole alkaloids as new cell cycle inhibitors and apoptosis inducers.
Cui
Cheng-Bin
CB
Beijing Institute of Biomedicine, Beijing 100091, People's Republic of China. cuicb@sohu.com
Yan
Shao-Yu
SY
Cai
Bing
B
Yao
Xin-Sheng
XS
D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001681:Biological Assay; D002227:Carbazoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D004249:DNA Damage; D005354:Fibrosarcoma / Q000473:pathology; D006801:Humans; D027821:Rutaceae / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000470:Alkaloids; D002227:Carbazoles; C110004:3-methylcarbazole