title: First Portuguese isolate of Neospora caninum from an aborted fetus from a dairy herd with endemic neosporosis.
abstract: Neospora caninum was isolated from the brain of an aborted 4-month-old fetus from a dairy cow herd with endemic neosporosis in Porto, Portugal. The fetal brain homogenate was inoculated interperitoneally first into outbred Swiss Webster mice given dexamethasone and then the peritoneal exudates from these mice was co-inoculated with mouse sarcoma cells in the peritoneal cavity of mice given dexamethasone. N. caninum tachyzoites were seen in peritoneal exudate of the second passage. Tachyzoites from the peritoneal exudate reacted positively with anti-N. caninum antibodies and not with anti-Toxoplasma gondii antibodies and contained N. caninum specific DNA. This Portuguese isolate of N. caninum has been successfully maintained in cell culture. The dam of the aborted fetus had an antibody titer of 1:10240 in the Neospora agglutination test (NAT). Antibodies to N. caninum were found in 76 of 106 cows from this herd in titers of 1:40 in 31, 1:80 in 22, > or =1:160 or more in 23 in the Neospora agglutination test. This is the first isolation of a viable N. caninum-like parasite from any host in Portugal.
authors.affiliation: Center for the Study of Animal Sciences (CECA/ICETA), University of Porto, Porto, Portugal.
authors.forename: Nuno
authors.initials: N
authors.lastname: Canada
authors.forename: Carla S
authors.initials: CS
authors.lastname: Meireles
authors.forename: António
authors.initials: A
authors.lastname: Rocha
authors.forename: Susana
authors.initials: S
authors.lastname: Sousa
authors.forename: Gertrude
authors.initials: G
authors.lastname: Thompson
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
authors.forename: S
authors.initials: S
authors.lastname: Romand
authors.forename: P
authors.initials: P
authors.lastname: Thulliez
authors.forename: J M
authors.initials: JM
authors.lastname: Correia da Costa
mesh_terms: D000034:Abortion, Veterinary / Q000469:parasitology*; D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001681:Biological Assay; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D003048:Coccidiosis / Q000097:blood / Q000469:parasitology / Q000662:veterinary*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D019353:Endemic Diseases; D005122:Exudates and Transudates / Q000469:parasitology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D051379:Mice; D018688:Neospora / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011174:Portugal; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000469:parasitology / Q000662:veterinary*
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan