title: Comparative analysis of telomere lengths and erosion with age in human epidermis and lingual epithelium.
abstract: We investigated progressive telomere shortening in normal human epidermis and lingual epithelium during aging, and attempted, in particular, to ascertain whether the telomere shortening that accompanies aging occurs at the same rate in different tissues. We studied telomeric DNA integrity, and estimated annual telomere loss, in 52 specimens of epidermis and 48 specimens of lingual epithelium collected at autopsy from subjects who had died at ages between 0 and 101 y. Most of the DNA samples were measured twice by southern blot hybridization. In addition, the correlation between telomere lengths in the two types of tissues was examined. The telomere reduction rates in epidermis and lingual epithelium were 36 bp and 30 bp per y, respectively, and these were significantly different. The rates obtained by the second measurements in epidermis and lingual epithelium were 39 and 32 bp per y, respectively, and these were also significantly different. The mean telomere lengths in the epidermis of eight neonates and the lingual epithelium of seven neonates were 13.2+/-1.0 and 13.8+/-1.0 kb, respectively. Comparison of telomere lengths in the two tissues for 41 paired samples showed that the mean telomere length in the epidermis (10.7+/-2.3 kb) was less than that in the lingual epithelium (12.4+/-2.5 kb); however, statistical analysis revealed a very significant relationship between epidermal and lingual epithelial telomere length (r=0.842, p<0.0001). These results indicate that the telomeres in epidermis and lingual epithelium are characterized by tissue-specific loss rates.
authors.affiliation: Department of Clinical Pathology, Tokyo Metropolitan Institute of Gerontology, Japan.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Nakamura
authors.forename: Naotaka
authors.initials: N
authors.lastname: Izumiyama-Shimomura
authors.forename: Motoji
authors.initials: M
authors.lastname: Sawabe
authors.forename: Tomio
authors.initials: T
authors.lastname: Arai
authors.forename: Yukitoshi
authors.initials: Y
authors.lastname: Aoyagi
authors.forename: Mutsunori
authors.initials: M
authors.lastname: Fujiwara
authors.forename: Eiju
authors.initials: E
authors.lastname: Tsuchiya
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Motonobu
authors.initials: M
authors.lastname: Kato
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Oshimura
authors.forename: Koji
authors.initials: K
authors.lastname: Sasajima
authors.forename: Kei
authors.initials: K
authors.lastname: Nakachi
authors.forename: Kaiyo
authors.initials: K
authors.lastname: Takubo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015139:Blotting, Southern; D002648:Child; D002675:Child, Preschool; D004817:Epidermis / Q000473:pathology*; D004848:Epithelium / Q000473:pathology; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D012044:Regression Analysis; D015595:Skin Aging / Q000473:pathology*; D016615:Telomere / Q000235:genetics / Q000473:pathology*; D014059:Tongue / Q000473:pathology*