Neural cell adhesion molecule promotes accumulation of TGN organelles at sites of neuron-to-neuron contacts.
Transformation of a contact between axon and dendrite into a synapse is accompanied by accumulation of the synaptic machinery at this site, being delivered in intracellular organelles mainly of TGN origin. Here, we report that in cultured hippocampal neurons, TGN organelles are linked via spectrin to clusters of the neural cell adhesion molecule (NCAM) in the plasma membrane. These complexes are translocated along neurites and trapped at sites of initial neurite-to-neurite contacts within several minutes after initial contact formation. The accumulation of TGN organelles at contacts with NCAM-deficient neurons is reduced when compared with wild-type cells, suggesting that NCAM mediates the anchoring of intracellular organelles in nascent synapses.
Sytnyk
Vladimir
V
Zentrum für Molekulare Neurobiologie, Universität Hamburg, D-20246 Hamburg, Germany.
Leshchyns'ka
Iryna
I
Delling
Markus
M
Dityateva
Galina
G
Dityatev
Alexander
A
Schachner
Melitta
M
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D020756:Coatomer Protein / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D019006:Neural Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D015388:Organelles / Q000378:metabolism*; D011726:Pyridinium Compounds / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013049:Spectrin / Q000378:metabolism; D013569:Synapses / Q000378:metabolism*; D016708:Synaptophysin / Q000378:metabolism; D013997:Time Factors; D021601:trans-Golgi Network / Q000378:metabolism*
D020756:Coatomer Protein; C092350:FM 4-64; D005456:Fluorescent Dyes; D019006:Neural Cell Adhesion Molecules; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D011993:Recombinant Fusion Proteins; D016708:Synaptophysin; D013049:Spectrin