title: Expression and location of the bradykinin B2 receptor in rat testis.
abstract: To investigate the possible role of the local tissue kallikrein-kinin system in spermatogenesis, we analyzed gene expression and cellular distribution of the bradykinin subtype-2 receptor (B(2) receptor) in the rat testis. Reverse transcription-polymerase chain reaction revealed B(2) receptor expression in testis and primary cultures of Sertoli cells and peritubular cells isolated from immature and mature rats. In situ hybridization of the B(2)-receptor mRNA showed intense labeling of cells on the base of the seminiferous tubule, whereas the autoradiographic signals gradually decreased toward the lumen. Immune histochemistry using testicular sections of pubertal and adult rats showed specific staining for the B(2)-receptor protein in cells of the adluminal compartment of the seminiferous tubules, especially on pachytene spermatocytes and spermatids. This immunostaining varied with the stages of the seminiferous cycle. The receptor protein was also observed on peritubular cells of pubertal rats. In conclusion, we demonstrated a stage-specific expression of the bradykinin B(2) receptor in different cells of the seminiferous tubules of the rat testis. The results point to a possible function of the tissue kallikrein-kinin system in the local regulation of spermatogenesis.
authors.affiliation: Center for Dermatology and Andrology, Justus Liebig University, D-35385 Giessen, Germany. thomas.monsees@derma.med.uni-giessen.de
authors.forename: Thomas K
authors.initials: TK
authors.lastname: Monsees
authors.forename: Sonja
authors.initials: S
authors.lastname: Blöcher
authors.forename: Frank
authors.initials: F
authors.lastname: Heidorn
authors.forename: Anett
authors.initials: A
authors.lastname: Winkler
authors.forename: Wolf-Eberhard
authors.initials: WE
authors.lastname: Siems
authors.forename: Werner
authors.initials: W
authors.lastname: Müller-Esterl
authors.forename: Jaleh
authors.initials: J
authors.lastname: Hayatpour
authors.forename: Werner
authors.initials: W
authors.lastname: Miska
authors.forename: Wolf-Bernhard
authors.initials: WB
authors.lastname: Schill
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000032:analysis; D015870:Gene Expression; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin / Q000032:analysis* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012671:Seminiferous Tubules / Q000737:chemistry / Q000166:cytology; D012708:Sertoli Cells / Q000737:chemistry; D012741:Sexual Maturation; D013087:Spermatids / Q000737:chemistry; D013090:Spermatocytes / Q000737:chemistry; D013091:Spermatogenesis; D013737:Testis / Q000737:chemistry*
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin