A tumor cell growth inhibitor from Saposhnikovae divaricata.
In the present study, we tested ethanolic extracts from 10 Chinese herbs for their effects on K562, Raji, Wish, HeLa, Calu-1, and Vero tumor cells proliferation. On a percentage basis, panaxynol purified from Saposhnikovae divaricata had the highest inhibitory activity on various tumor cells proliferation. Cell-cycle analysis indicated that panaxynol arrested the cell cycle progression of tumor cells from the G1 transition to the S phase. In an attempt to further localize the point in the cell cycle where arrest occurred, gene expression of cyclin E, a key regulatory event leading to the G1/S boundary was examined. Results indicated that the levels of cyclin E mRNA in various tumor cells were decreased by panaxynol. Thus, the suppressant effects of panaxynol on proliferation of various tumor cells appeared to be mediated, at least in part, through impairments of cyclin E mRNA levels and arresting cell cycle progression in the cells.
Kuo
Yuh-Chi
YC
National Research Institute of Chinese Medicine, Department of Biology, Fu Jen University, Taipei, Taiwan, ROC.
Lin
Yun-Lian
YL
Huang
Cheng-Po
CP
Shu
Jia-Wei
JW
Tsai
Wei-Jern
WJ
D000480:Alkynes; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D019661:Apiaceae / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019927:Cyclin E / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D053280:Diynes; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005233:Fatty Alcohols / Q000302:isolation & purification / Q000494:pharmacology*; D016193:G1 Phase / Q000187:drug effects; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism
D000480:Alkynes; D000970:Antineoplastic Agents; D019927:Cyclin E; D017931:DNA Primers; D053280:Diynes; D004365:Drugs, Chinese Herbal; D005233:Fatty Alcohols; D010936:Plant Extracts; D012333:RNA, Messenger; C018541:falcarinol