Involvement of in vivo induced cheY-4 gene of Vibrio cholerae in motility, early adherence to intestinal epithelial cells and regulation of virulence factors.
Using a global transcription profile approach cheY-4 of Vibrio cholerae was identified as an in vivo induced gene. In the present study, duplication of the gene in the chromosome resulted in increased motility, increased chemotactic response towards isolated intestinal mucus layer and stronger adhesion to human intestinal epithelial cell line at an early phase of infection compared to wild type and a null mutant strain. In contrast to the cheY-4 null mutant, duplication of cheY-4 gene resulted in increased expression of ctxAB and tcpA, the two major virulence genes of V. cholerae.
Banerjee
Rajat
R
Biophysics Division, Indian Institute of Chemical Biology 4, Raja S.C. Mullick Road, Jadavpur, Calcutta-700 032, India.
Das
Soumita
S
Mukhopadhyay
Kanchan
K
Nag
Sanjay
S
Chakrabortty
Amit
A
Chaudhuri
Keya
K
D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology*; D001483:Base Sequence; D002460:Cell Line; D002772:Cholera Toxin / Q000096:biosynthesis / Q000235:genetics; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000382:microbiology / Q000648:ultrastructure; D036781:Fimbriae Proteins / Q000096:biosynthesis / Q000235:genetics; D040461:Gene Components; D020440:Gene Duplication; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006168:Guinea Pigs; D007413:Intestinal Mucosa / Q000382:microbiology*; D007700:Kinetics; D008124:Locomotion; D008969:Molecular Sequence Data; D009154:Mutation; D012329:RNA, Bacterial / Q000096:biosynthesis; D014158:Transcription, Genetic; D014734:Vibrio cholerae / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D037521:Virulence Factors / Q000096:biosynthesis* / Q000235:genetics
D001426:Bacterial Proteins; C470183:CheY-4 protein, Vibrio cholerae; D012329:RNA, Bacterial; C085807:TcpA protein, Vibrio cholerae; D037521:Virulence Factors; D036781:Fimbriae Proteins; D002772:Cholera Toxin