title: A rat monoclonal antibody that catalyses the hydrolysis of a nitrophenyl-beta-lactam.
abstract: We report the first example of a monoclonal antibody-catalysed hydrolysis of a beta-lactam where the antibodies were generated by a simple transition-state analogue. A rat monoclonal antibody (1/91c/4d/26) generated by using an acyclic 4-nitrophenylphosphate immunogen catalysed the hydrolysis of corresponding 4-nitrophenyl carbonates but, more importantly, also catalysed the hydrolysis of N-(4-nitrophenyl)-azetidinone at pH 8 with k(cat)=8.7 x 10(-6)s(-1) and K(M)=35 microM. This is the first example of a rat monoclonal catalytic antibody.
authors.affiliation: School of Pharmacy and Biomolecular Sciences, University of Brighton, Cockcroft Building, Moulsecoomb, BN2 4GJ, Brighton, UK.
authors.forename: Elizabeth L
authors.initials: EL
authors.lastname: Ostler
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Dean
authors.forename: Nicola
authors.initials: N
authors.lastname: Barber
authors.forename: Maurizio
authors.initials: M
authors.lastname: Valeri
authors.forename: Stuart
authors.initials: S
authors.lastname: James
authors.forename: Marina
authors.initials: M
authors.lastname: Resmini
authors.forename: Guillaume
authors.initials: G
authors.lastname: Boucher
authors.forename: Nickolas
authors.initials: N
authors.lastname: Romanov
authors.forename: Keith
authors.initials: K
authors.lastname: Brocklehurst
authors.forename: Gerard
authors.initials: G
authors.lastname: Gallacher
mesh_terms: D000818:Animals; D017151:Antibodies, Catalytic / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002384:Catalysis; D006868:Hydrolysis; D007700:Kinetics; D009596:Nitrophenols / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D047090:beta-Lactams / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
chemical_list: D017151:Antibodies, Catalytic; D000911:Antibodies, Monoclonal; D009596:Nitrophenols; D047090:beta-Lactams; C024719:4-nitrophenyl