title: Inhibitory effect of chlorophyllin on the frequency of micronuclei induced by sodium nitrite in mice.
abstract: In this report the potency of chlorophyllin (CHL) was evaluated to prevent two types of damage produced by nitrite in mice: the increase of micronucleated polychromatic erythrocytes (MNPE) and the bone marrow toxicity, measured as the index of polychromatic erythrocytes/normochromatic erythrocytes (PE/NE). The study was done in eight groups of male mice. The first three groups were administered orally for 4 days with sodium nitrite (10, 15 and 20 mg/kg), the daily administration with nitrite was followed by an intraperitoneal administration of CHL (4 mg/kg), three more groups were administered with the same amounts of nitrite, a seventh group of mice was treated with distilled water while another was treated with CHL (4 mg/kg). Our study produced two main results: (a) no bone marrow injury was induced by any of the tested chemicals, as indicated with the PE/NE index, and (b) CHL protected (as high as 44%) the MNPE produced in nitrite treated mice.
authors.affiliation: Laboratorio de Citogenética FESC-UNAM, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México.
authors.forename: S
authors.initials: S
authors.lastname: Diaz-Barriga Arceo
authors.forename: A
authors.initials: A
authors.lastname: Hernández-Ceruelos
authors.forename: E
authors.initials: E
authors.lastname: Madrigal-Bujaidar
authors.forename: G
authors.initials: G
authors.lastname: Chamorro
mesh_terms: D000284:Administration, Oral; D000818:Animals; D016587:Antimutagenic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002735:Chlorophyllides / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects* / Q000473:pathology; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects / Q000473:pathology; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens / Q000008:administration & dosage / Q000494:pharmacology*; D008517:Phytotherapy; D012977:Sodium Nitrite / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D016587:Antimutagenic Agents; D002735:Chlorophyllides; D009153:Mutagens; C007020:chlorophyllin; D012977:Sodium Nitrite