Direct electron transfer between hemoglobin and a glassy carbon electrode facilitated by lipid-protected gold nanoparticles.
We synthesized a kind of gold nanoparticle protected by a synthetic lipid (didodecyldimethylammonium bromide, DDAB). With the help of these gold nanoparticles, hemoglobin can exhibit a direct electron transfer (DET) reaction. The formal potential locates at -169 mV vs. Ag/AgCl. Spectral data indicated the hemoglobin on the electrode was not denatured. The lipid-protected gold nanoparticles were very stable (for at least 8 months). Their average diameter is 6.42 nm. It is the first time to use monolayer-protected nanoparticles to realize the direct electrochemistry of protein.
Han
Xiaojun
X
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 159 Renmin Street, Jilin 130022, Changchun, China.
Cheng
Wenlong
W
Zhang
Zheling
Z
Dong
Shaojun
S
Wang
Erkang
E
D002244:Carbon / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D006046:Gold / Q000737:chemistry*; D006427:Hemin / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry*; D008055:Lipids / Q000138:chemical synthesis / Q000737:chemistry*; D010316:Particle Size; D013057:Spectrum Analysis / Q000379:methods
D006454:Hemoglobins; D008055:Lipids; D006427:Hemin; D002244:Carbon; D006046:Gold