Estrogenic activity in water and sediments of a French river: contribution of alkylphenols.
Alkylphenols, known to possess estrogenic activity, have been found in the aquatic environment. In this study, we focused on the contribution of alkylphenols to total estrogenic activity in sediment and water extracts of French rivers. Four sites representing rural, agricultural, urban, and industrial watersheds were studied. The concentrations of alkylphenols in water and sediment were quantified by GC/MS. Estrogen-responsive reporter cell lines (MELN) have been used for investigating estrogenic activity at these sites. These observed activities were compared with activities mediated by known concentrations of alkylphenols. In water, the concentration of alkylphenols, from 0.06 to 0.550 microg x L(-1) and from < 0.001 microg x L(-1) to 0.077 microg x L(-1) for nonylphenols and 4t-octylphenol, respectively, were too low to contribute to the observed estrogenic activity. In sediment of the industrial, rural, and urban sites, the observed estrogenic activities could be explained in great part by the alkylphenol concentrations from 0.26 to 2.87 microg x g(-1) and from 0.005 microg x g(-1) to 0.49 microg x g(-1) for nonylphenols and 4t-octylphenol, respectively. In the agricultural site, the alkylphenols (0.022 microg x g(-1) of nonylphenols) poorly contribute to the observed estrogenic activity. Other compounds, such as natural and synthetic hormones, present in water and sediments could act additively in the overall activity.
Fenet
H
H
Laboratoire de chimie analytique, Faculté de Pharmacie, Montpellier, France.
Gomez
E
E
Pillon
A
A
Rosain
D
D
Nicolas
J-C
JC
Casellas
C
C
Balaguer
P
P
D000383:Agriculture; D001943:Breast Neoplasms / Q000473:pathology; D002947:Cities; D004703:Endocrine System / Q000187:drug effects; D004967:Estrogens / Q000096:biosynthesis* / Q000494:pharmacology; D005602:France; D008401:Gas Chromatography-Mass Spectrometry; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter; D019015:Geologic Sediments / Q000737:chemistry; D006801:Humans; D007221:Industry; D010636:Phenols / Q000009:adverse effects*; D010957:Plasmids; D014162:Transfection; D014407:Tumor Cells, Cultured; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
D004967:Estrogens; D010636:Phenols; D014874:Water Pollutants, Chemical