title: Blood copper concentrations and cuproenzyme activities in a colony of cats.
abstract: BACKGROUND
  There is little published information on plasma copper and whole blood copper concentrations in cats, and except for extracellular superoxide dismutase (EC SOD), we are unaware of published values for cat cuproenzyme activities.
  
  
  OBJECTIVE
  In this study we determined plasma and whole blood copper concentrations, plasma ceruloplasmin (CP), EC SOD and diamine oxidase (DAO) activities, and erythrocyte superoxide dismutase (RBC SOD) activity in a specific pathogen-free colony of cats. Results were evaluated for differences based on age and sex.
  
  
  METHODS
  Blood was obtained from 20 adult cats (10 males, 10 females; >1 year of age) and 20 immature cats (10 males, 10 females; <1 year of age). Copper concentrations were determined using atomic absorption spectrophotometry. EC SOD and RBC SOD activities were determined using the pyrogallol oxidation method, and CP activity was determined by the oxidation of 0 dianisidine dihydrochloride. DAO activity was measured using a colorimetric assay. Differences among groups were analyzed using ANOVA.
  
  
  RESULTS
  There were significant differences in mean plasma copper concentration, and CP and DAO activities (P=.001,.0001, and.02, respectively) among the 4 groups of cats. No age differences were identified. Male cats had significantly greater mean (+/- SEM) plasma copper (15.4 +/- 0.9 micromol/L versus 11.3 +/- 0.6 micromol/L; P =.001) concentrations, and CP (66.8 +/- 2.9 U/L versus 39.7 +/- 2.1 U/L; P=.0001) and DAO (6.68 +/- 0.16 U/L versus 6.15 +/- 0.1 U/L; P =.03) activities compared with female cats. Males had significantly greater whole blood copper concentrations (16.16 +/- 0.55 micromol/L versus 13.36 +/- 0.52 micromol/L; P =.002) than female cats.
  
  
  CONCLUSIONS
  Differences exist between male and female cats with respect to blood copper concentrations, and CP and DAO activities.
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis 95616-8741, USA. ajfascetti@ucdavis.edu
authors.forename: Andrea J
authors.initials: AJ
authors.lastname: Fascetti
authors.forename: Quinton R
authors.initials: QR
authors.lastname: Rogers
authors.forename: James G
authors.initials: JG
authors.lastname: Morris
mesh_terms: D000375:Aging; D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D000818:Animals; D002415:Cats / Q000097:blood*; D002570:Ceruloplasmin / Q000032:analysis*; D003300:Copper / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D008297:Male; D012737:Sex Factors; D013047:Specific Pathogen-Free Organisms; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D003300:Copper; D013482:Superoxide Dismutase; D002570:Ceruloplasmin; D006631:Amine Oxidase (Copper-Containing)