[Matrine affects early expression of proto-oncogenes in K562 cells].
OBJECTIVE
It has been reported that matrine had the anti-tumor activity, and our previous study have provided that matrine had the effects of inhibiting proliferation and inducing differentiation in K562 cell line, but its molecular mechanism is unknown yet.


METHODS
The expression of several proto-oncogenes(c-myc, c-jun, H-ras, p21, and HNF-1 alpha) in K562 cell line treated by 0.2 mg/ml matrine were measured by RT-PCR.


RESULTS
The c-myc, c-jun, and HNF-1 alpha mRNA of K562 cells treated by 0.2 mg/ml matrine were dramatically decreased at the early stage (3 h), while the H-ras and p21 mRNA were increased obviously at the same time.


CONCLUSIONS
The changes of several proto-oncogenes in K562 cells treated by 0.2 mg/ml matrine may be related to inhibiting proliferation and inducing differentiation.
He
Yu-juan
YJ
Department of Clinical Bio-chemistry, Chongqing University of Medical Sciences, Chongqing 400016, P. R. China. hyj2753@hotmail.com
Jiang
Ji-kai
JK
Ou
Yi-heng
YH
Liu
Bei-zhong
BZ
Liu
Xiao-shan
XS
Zhang
Yan
Y
Ma
Lin-di
LD
Tu
Zhi-guang
ZG
D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004268:DNA-Binding Proteins; D015870:Gene Expression / Q000187:drug effects*; D011905:Genes, ras / Q000187:drug effects / Q000235:genetics; D051537:Hepatocyte Nuclear Factor 1; D051538:Hepatocyte Nuclear Factor 1-alpha; D051539:Hepatocyte Nuclear Factor 1-beta; D006801:Humans; D020014:K562 Cells; D009687:Nuclear Proteins; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics; D011519:Proto-Oncogenes / Q000187:drug effects* / Q000235:genetics; D011807:Quinolizines; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D000093842:Matrines
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D004268:DNA-Binding Proteins; C496487:HNF1A protein, human; C496495:HNF1B protein, human; D051538:Hepatocyte Nuclear Factor 1-alpha; D009687:Nuclear Proteins; D016755:Proto-Oncogene Proteins c-jun; D016271:Proto-Oncogene Proteins c-myc; D011807:Quinolizines; D012333:RNA, Messenger; D014157:Transcription Factors; D051537:Hepatocyte Nuclear Factor 1; D051539:Hepatocyte Nuclear Factor 1-beta; D000093842:Matrines