Dose-responsive alteration in hepatic lipid peroxidation and retinol metabolism with increasing dietary beta-carotene in iron deficient rats.
Phosphatidylcholine hydroperoxide (PCOOH) levels are increased in the iron-deficient rat liver. We investigated the antioxidative effect of dietary beta-carotene and altered retinol metabolism in iron-deficient rats. Experiment 1: Male Wistar-strain rats were divided into six groups and fed a control diet, an iron-deficient diet, and iron-deficient diets with four different levels of dietary beta-carotene. The PCOOH concentration in the iron-deficient rat liver was decreased by supplementation with dietary beta-carotene. However, the beta-carotene dose response was not related to antioxidative potency. Hepatic and plasma beta-carotene concentrations were increased by iron deficiency. The hepatic retinol concentration was increased while the plasma retinol concentration was decreased in iron-deficient rats. Experiment 2: Male Wistar-strain rats were divided into two groups, with one group receiving a control diet with beta-carotene and the other an iron-deficient diet with beta-carotene. Intestinal iron was decreased and intestinal beta-carotene was unchanged in iron-deficient rats. The intestinal beta-carotene conversion ratio and beta-carotene cleavage enzyme activity were decreased in iron-deficient rats. Dietary beta-carotene played the role of an antioxidant in hepatic lipid peroxidation in the iron-deficient state, but there was no dose dependency. Moreover, intestinal beta-carotene cleavage and hepatic retinol release appear to be altered in iron-deficient rats.
Ikeda
Ryouko
R
Department of Nutritional Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-Ku, Tokyo 156-8502, Japan.
Uehara
Mariko
M
Takasaki
Misao
M
Chiba
Hiroshige
H
Masuyama
Ritsuko
R
Furusho
Tadasu
T
Suzuki
Kazuharu
K
D018798:Anemia, Iron-Deficiency / Q000378:metabolism* / Q000628:therapy*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D004032:Diet; D004305:Dose-Response Relationship, Drug; D006454:Hemoglobins / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014801:Vitamin A / Q000378:metabolism*; D015430:Weight Gain / Q000187:drug effects; D019207:beta Carotene / Q000008:administration & dosage* / Q000097:blood
D000975:Antioxidants; D006454:Hemoglobins; D010713:Phosphatidylcholines; C057590:phosphatidylcholine hydroperoxide; D019207:beta Carotene; D014801:Vitamin A; D007501:Iron