Amphioxus homologs of Go-coupled rhodopsin and peropsin having 11-cis- and all-trans-retinals as their chromophores.
Because of low contents in the native organs and failure of the expression in cultured cells, the chromophore configurations of the pigments in Go-coupled opsin and peropsin groups in the opsin family are unknown. Here we have succeeded in expression of the amphioxus homologs of these groups in HEK293s cells and found that they can be regenerated with 11-cis- and all-trans-retinals, respectively. Light isomerized the chromophores of these opsins into the all-trans and 11-cis forms, respectively. The results strongly suggest that the physiological function of peropsin would be a retinal photoisomerase, while 11-cis configuration is necessary for the Go-coupled opsin groups.
Koyanagi
Mitsumasa
M
Department of Biophysics, Graduate School of Science, Kyoto University, 606-8502, Kyoto, Japan.
Terakita
Akihisa
A
Kubokawa
Kaoru
K
Shichida
Yoshinori
Y
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D005136:Eye Proteins / Q000737:chemistry*; D019204:GTP-Binding Proteins / Q000737:chemistry*; D006801:Humans; D010802:Phylogeny; D012172:Retinaldehyde / Q000737:chemistry*; D012243:Rhodopsin / Q000737:chemistry*; D014212:Tretinoin / Q000737:chemistry*
D005136:Eye Proteins; C504671:RRH protein, human; D014212:Tretinoin; D012243:Rhodopsin; D019204:GTP-Binding Proteins; D012172:Retinaldehyde