title: Measurement of H2'-C2' and H3'-C3' dipolar couplings in RNA molecules.
abstract: The quality of nucleic acid solution structures can be significantly improved using residual dipolar coupling data. However, many of the one-bond couplings that could be used for this purpose are difficult to measure. Conventional 2D experiments are often unable to reveal one-bond H2'-C2' and H3'-C3' couplings in large RNA molecules due to spectral overlap. Here we show how to use 3D HCcH-COSY and Relay HCcH-COSY to measure one-bond H2'-C2' and H3'-C3' couplings which improved the precision of the structures obtained recently for a 42 nucleotide RNA.
authors.affiliation: Department of Chemistry, Yale University, New Haven, CT 06520-8107, USA.
authors.forename: Pramodh
authors.initials: P
authors.lastname: Vallurupalli
authors.forename: Peter B
authors.initials: PB
authors.lastname: Moore
mesh_terms: D002247:Carbon Isotopes; D031541:Fluorescence Resonance Energy Transfer; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D011522:Protons; D012313:RNA / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D011522:Protons; D012313:RNA