The expression of ADAM12 (meltrin alpha) in human giant cell tumours of bone.
OBJECTIVE
To examine the expression of ADAM12 (meltrin alpha), a member of the disintegrin and metalloprotease (ADAM) family, in human giant cell tumours of the bone, skeletal muscle tissue from human embryos, and human adult skeletal muscle tissue.


METHODS
ADAM12 mRNA was detected by reverse transcription polymerase chain reaction and in situ hybridisation.


RESULTS
ADAM12 mRNA was detected in 14 of the 20 giant cell tumours of bone and in three of the six tumour cell cultures. The expression of ADAM12 in cells cultured from the tumour was linked to the presence of multinucleated giant cells. ADAM12 mRNA could not be detected in the five adult skeletal muscle tissue samples, although it was found in the two embryonic skeletal muscle tissue samples. ADAM12 mRNA was localised to the cytoplasm of multinucleated giant cells and some mononuclear stromal cells.


CONCLUSIONS
These results indicate that multinucleated giant cells are formed by the cell fusion of mononuclear stromal cells in giant cell tumours of bone and that ADAM12 is involved in the cell fusion process.
Tian
B L
BL
Department of Pathology, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, People's Republic of China.
Wen
J M
JM
Zhang
M
M
Xie
D
D
Xu
R B
RB
Luo
C J
CJ
D051722:ADAM Proteins; D000072199:ADAM12 Protein; D000328:Adult; D001859:Bone Neoplasms / Q000378:metabolism*; D019483:Disintegrins / Q000378:metabolism; D015870:Gene Expression; D018212:Giant Cell Tumor of Bone / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D019483:Disintegrins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D051722:ADAM Proteins; D000072199:ADAM12 Protein; C000606480:ADAM12 protein, human; D008666:Metalloendopeptidases