title: Excess dNTPs minimize RNA hydrolysis during reverse transcription.
authors.affiliation: Invitrogen, Frederick, MD, USA. ggerard@transgenomic.com
authors.forename: Gary F
authors.initials: GF
authors.lastname: Gerard
authors.forename: Sarah
authors.initials: S
authors.lastname: Collins
authors.forename: Michael D
authors.initials: MD
authors.lastname: Smith
mesh_terms: D001358:Avian Sarcoma Viruses / Q000201:enzymology; D018076:DNA, Complementary / Q000096:biosynthesis*; D003838:Deoxyadenine Nucleotides / Q000494:pharmacology*; D003845:Deoxycytosine Nucleotides / Q000494:pharmacology*; D003848:Deoxyguanine Nucleotides / Q000494:pharmacology*; D004355:Drug Stability; D004587:Electrophoresis, Agar Gel; D006207:Half-Life; D006358:Hot Temperature; D006868:Hydrolysis / Q000187:drug effects; D008274:Magnesium / Q000494:pharmacology; D008979:Moloney murine leukemia virus / Q000201:enzymology; D009691:Nucleic Acid Denaturation; D012313:RNA / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D013942:Thymine Nucleotides / Q000494:pharmacology*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D003838:Deoxyadenine Nucleotides; D003845:Deoxycytosine Nucleotides; D003848:Deoxyguanine Nucleotides; D013942:Thymine Nucleotides; D014764:Viral Proteins; C024107:2'-deoxycytidine 5'-triphosphate; D012313:RNA; C029603:deoxyguanosine triphosphate; D012194:RNA-Directed DNA Polymerase; D008274:Magnesium; C026600:2'-deoxyadenosine triphosphate; C024157:thymidine 5'-triphosphate