New PCR-RFLP analysis for the mouse Tnfsf6gld gene caused by a point mutation in the Tnfsf6 [tumor necrosis factor (ligand) superfamily, member 6] locus.
A new polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was developed for genetic typing of the mouse Tnfsf6gld mutation. An artificial restriction site was introduced to the mouse Tnfsf6gld mutation by PCR amplification using a modified primer. The three genotypes of the Tnfsf6 locus (Tnfsf6gld/Tnfsf6gld, Tnfsf6gld/+, and +Tnfsf6-gld/+Tnfsf6-gld) could be distinguished clearly and easily. This PCR-RFLP analysis was found to be useful for the identification of the mouse Tnfsf6gld mutation.
Masui
Norio
N
Japan SLC, Inc., 3-5-1 Aoihigashi, Hamamatsu, Shizuoka 433-8114, Japan.
Takagi
Yumie
Y
Nishikawa
Tetsu
T
Yanabe
Makoto
M
Nose
Masato
M
Sato
Katsunori
K
D000818:Animals; D002874:Chromosome Mapping; D004252:DNA Mutational Analysis / Q000379:methods; D017931:DNA Primers; D005838:Genotype; D051379:Mice; D008809:Mice, Inbred C3H / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D017931:DNA Primers; D014409:Tumor Necrosis Factor-alpha