Inflammatory response to different endodontic irrigating solutions.
OBJECTIVE
The aim of this study was to evaluate the inflammatory response to irrigating solutions injected into the peritoneal cavity of mice.


METHODS
Sixty mice received intra-peritoneal injections of 0.3 mL of 0.5% sodium hypochlorite, 2.0% chlorhexidine digluconate or phosphate buffered saline (PBS, control). Five animals of each group were sacrificed at 4, 24, 48 h and 7 days after the injection. Liquid from the peritoneal cavity of each animal was collected for the total and differential counting of inflammatory cells and protein leakage.


RESULTS
The 0.5% sodium hypochlorite solution group had greater migration of neutrophils and mononuclear cells to the peritoneal cavity from 48 to 168 h (P < 0.05). There was a significant increase in protein leakage to the peritoneal cavity after 4 up to 48 h in the 0.5% sodium hypochlorite group compared to the control group. Protein leakage was similar in all groups at 168 h. The 2.0% chlorhexidine group had similar results to the control group at all time periods.


CONCLUSIONS
The 0.5% sodium hypochlorite solution induced an inflammatory response, however, the 2.0% chlorhexidine digluconate solution did not induce a significant inflammatory response.
Tanomaru Filho
M
M
Department of Endodontics, School of Dentistry of Araraquara, University of the State of São Paulo, Araraquara, SP, Brazil.
Leonardo
M R
MR
Silva
L A B
LA
Aníbal
F F
FF
Faccioli
L H
LH
D000818:Animals; D001202:Ascitic Fluid / Q000139:chemically induced / Q000473:pathology; D001672:Biocompatible Materials / Q000009:adverse effects*; D002465:Cell Movement; D002710:Chlorhexidine / Q000009:adverse effects / Q000031:analogs & derivatives*; D004202:Disinfectants / Q000009:adverse effects; D007274:Injections, Intraperitoneal; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000473:pathology; D008297:Male; D051379:Mice; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000473:pathology; D010529:Peritoneal Cavity / Q000473:pathology*; D011506:Proteins / Q000032:analysis; D011897:Random Allocation; D012388:Root Canal Irrigants / Q000009:adverse effects*; D012965:Sodium Chloride; D012973:Sodium Hypochlorite / Q000009:adverse effects; D018709:Statistics, Nonparametric; D013997:Time Factors
D001672:Biocompatible Materials; D004202:Disinfectants; D011506:Proteins; D012388:Root Canal Irrigants; D012965:Sodium Chloride; D012973:Sodium Hypochlorite; C010882:chlorhexidine gluconate; D002710:Chlorhexidine