title: Invasion of bovine epithelial cells by verocytotoxin-producing Escherichia coli O157:H7.
abstract: The ability of verocytotoxin-producing Escherichia coli (VTEC) O157:H7 to enter selected human (RPMI-4788 and HeLa) and bovine (MAC-T, mammary secretory; MDBK, kidney) epithelial cell lines was evaluated. All VTEC evaluated efficiently entered RPMI-4788 and MAC-T cell lines. VTEC entered MDBK cells at approximately 4% of MAC-T cells. VTEC were not able to invade HeLa cells. Presence of plasmid had no influence on efficiency of entry, nor did production of shiga-like toxin (SLT I or SLT II). Internalization required microfilaments, but not microtubules. Two types of adherence, localized and diffuse, were exhibited depending on isolate and cell line evaluated. Ability of VTEC to invade bovine mammary epithelial cells may be important in pathogenesis in the bovine, may indicate a route by which raw milk may potentially become contaminated, and may provide a reservoir of bacteria for the contamination of workers, equipment and carcass at time of slaughter.
authors.affiliation: Department of Food Science, Rutgers University, New Brunswick, NJ 08903-0231, USA.
authors.forename: K R
authors.initials: KR
authors.lastname: Matthews
authors.forename: P A
authors.initials: PA
authors.lastname: Murdough
authors.forename: A J
authors.initials: AJ
authors.lastname: Bramley
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002417:Cattle; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000378:metabolism / Q000502:physiology*; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D022622:Shiga Toxin 1 / Q000096:biosynthesis*
chemical_list: D022622:Shiga Toxin 1