title: Single-cell imaging of the Ca2+ influx into bovine endothelial cells occurring in response to an alternating electric stimulus.
abstract: The electric control of cellular functions via Ca2+ was formerly suggested. From this viewpoint, the involvement of a Ca2+ channel was studied using bovine fetal arterial endothelial (BFAE) cells in which P2X4, an ATP-operated and fluid shear stress sensitive Ca2+ channel, exists predominantly. An electric stimulus (sine wave, 10 Hz, 10 VPP, 30 s) caused a marked influx of Ca2+ into BFAE cells from an extracellular solution. The magnitude of the [Ca2+]i change increased with a decrease in the frequency in the range from 100 Hz to 5 Hz. Regarding the pathway of this Ca2+ influx, single-cell imaging and an ATP depletion experiment strongly suggested the involvement of a pathway different from P2X4. This pathway was thought to be a non-specific one, because typical Ca2+ channel blockers, such as verapamil, Gd3+, and Co2+, could not inhibit the Ca2+ influx.
authors.affiliation: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Matsuoka
authors.forename: Kenji
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Horikiri
authors.forename: Mikako
authors.initials: M
authors.lastname: Saito
authors.forename: Kimiko
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Joji
authors.initials: J
authors.lastname: Ando
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects; D002417:Cattle; D002478:Cells, Cultured; D004558:Electric Stimulation; D004727:Endothelium / Q000166:cytology* / Q000378:metabolism*; D013314:Stress, Mechanical; D013997:Time Factors
chemical_list: D002121:Calcium Channel Blockers; D002118:Calcium