Molecular cloning and characterization of a novel human gene (ANP32E alias LANPL) from human fetal brain.
Leucine-rich acidic nuclear protein (LANP) is a member of the leucine-rich repeats (LRRs) superfamily. Here we report on a human homologue of LANP, encoded by the gene ANP32E (alias LANPL). The gene was cloned and identified during large-scale sequencing analysis of a human fetal brain cDNA library. The human protein shared 70% amino acid identity with rat LANP. According to bioinformatics analysis, ANP32E is located on chromosome 1q22. RT-PCR analysis indicates that ANP32E was expressed in human peripheral blood leukocytes, colon, small intestine, prostate, thymus, spleen, skeletal muscle, liver and kidney.
Jiang
M
M
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, P.R. China.
Ma
Y
Y
Ni
X
X
Cao
G
G
Ji
C
C
Cheng
H
H
Tang
R
R
Xie
Y
Y
Mao
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D005333:Fetus / Q000378:metabolism; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D008297:Male; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D010750:Phosphoproteins; D011247:Pregnancy; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014018:Tissue Distribution
C490336:ANP32E protein, human; C490338:Anp32a protein, rat; D018076:DNA, Complementary; D018832:Molecular Chaperones; D009479:Neuropeptides; D009687:Nuclear Proteins; D010750:Phosphoproteins