[Impact of Yersinia pseudotuberculosis on the in vitro production of cytokines by whole blood cells of donors].
The impact of two plasmid (47, 82 MD), single plasmid (47 MD) and non plasmid Y. pseudotuberculosis strains, Y. enterocolitica (47 MD) as well as Y. pseudotuberculosis superantigen (YPM) on the production of interleukin-1 (IL-1), interleukin-6 (IL-6), interferon-alpha (IFN = alpha) and tumor necrosis factor alpha (TNF-alpha) by whole blood cells obtained from donors was studied. All Y. pseudotuberculosis and Y. enterocolitica strains stimulated the production of IFN-alpha, IL-1, IL-6 and TNF-alpha by whole blood cells, but considerably less than Y. pseudotuberculosis lipopolysaccharide and YPM. These data are indicative of the pathogenetic role played by 82 MD plasmid in manifestation of Y. pseudotuberculosis immunosuppressive properties. The maximum stimulation of the production of cytokines was observed under the action of YPM, which confirmed an important role played by this superantigen in the pathogenesis of Y. pseudotuberculosis.
Voropaev
A V
AV
State Medical University, Research Institute for Plague Control of Siberia and the Far East, Irkutsk, Russia.
Shurygina
I A
IA
Klimov
V T
VT
Chesnokova
M V
MV
Maramovich
A S
AS
Malov
I V
IV
Kirdeĭ
E G
EG
D000293:Adolescent; D001426:Bacterial Proteins / Q000494:pharmacology; D001773:Blood Cells / Q000276:immunology / Q000382:microbiology*; D001782:Blood Donors; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D007124:Immunoenzyme Techniques; D016898:Interferon-alpha / Q000032:analysis / Q000096:biosynthesis; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis; D015850:Interleukin-6 / Q000032:analysis / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008970:Molecular Weight; D010957:Plasmids / Q000494:pharmacology; D018089:Superantigens / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000096:biosynthesis; D015011:Yersinia pseudotuberculosis / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*
D001426:Bacterial Proteins; D016207:Cytokines; D016898:Interferon-alpha; D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D018089:Superantigens; D014409:Tumor Necrosis Factor-alpha; C093313:Yersinia pseudotuberculosis-derived mitogen