Identification of the epitope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus.
In order to identify the neutralizing epitope of the porcine epidemic diarrhea virus (PEDV), the spike protein region that is presumed to contain the virus-neutralizing epitope was determined. This was based on the sequence information for the neutralizing epitope of the transmissible gastroenteritis virus (TGEV). A recombinant protein that corresponds to the spike region of TGEV was produced, and polyclonal antisera were generated using the recombinant protein. It was discovered that polyclonal antisera significantly inhibited plaque formation by PEDV, suggesting that this region of the spike protein contains the epitope(s) that is capable of inducing PEDV-neutralizing antibodies. In addition, the region that corresponds to the neutralizing epitope of TGEV may also be involved in neutralizing PEDV, although the two viruses are serologically quite distinct. Finally, the amino acid sequences that are deduced from the genes for the determined-neutralizing epitope were highly homologous among the PEDV strains that were isolated from different geographical areas, which suggests conservation of the antigen gene.
Chang
Sun-Hwa
SH
Division of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju, Korea.
Bae
Jong-Lye
JL
Kang
Tae-Jin
TJ
Kim
Ju
J
Chung
Gook-Hyun
GH
Lim
Chae-Woong
CW
Laude
Hubert
H
Yang
Moon-Sik
MS
Jang
Yong-Suk
YS
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D017934:Coronavirus / Q000276:immunology*; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology*; D005761:Gastroenteritis, Transmissible, of Swine / Q000276:immunology* / Q000821:virology*; D008969:Molecular Sequence Data; D013552:Swine / Q000276:immunology / Q000821:virology
D000906:Antibodies; D000941:Antigens; D000939:Epitopes