Rifampin- and multidrug-resistant tuberculosis in Russian civilians and prison inmates: dominance of the beijing strain family.
Consecutive patient cultures (140) of Mycobacteriium tuberculosis were collected from five Russian civilian and prison tuberculosis laboratories and analyzed for rifampin (rpoB) and isoniazid resistance (inhA, katG, ahpC); transmission of Beijing family isolates; and the importance of prison and previous therapy in drug resistance. Rifampin, isoniazid, and multidrug resistance occurred in 58.2%, 51.6%, and 44.7% of cultures, respectively; 80% of prison cultures were rifampin resistant. Spoligotyping and variable number tandem repeat (VNTR) fingerprinting divided the isolates into 43 groups. Spoligotyping demonstrated that a high proportion (68.1%) of patients were infected with Beijing family strains and that most (69.1%) were rifampin resistant; the highest proportion (81.6%) occurred in prison. One VNTR subgroup (42435) comprised 68 (72.3%) of the Beijing isolates with a small number of IS6110 types; 50 (73.5%) were rifampin resistant. Rifampin-resistant Beijing isolates are dominant within the patient population, especially among prisoners, and threaten treatment programs.
Drobniewski
Francis
F
Guy's King's amd St Thomas' Medical School, King's College Hospital, London, United Kingdom. francis.drobniewski@kcl.ac.uk
Balabanova
Yanina
Y
Ruddy
Michael
M
Weldon
Laura
L
Jeltkova
Katya
K
Brown
Timothy
T
Malomanova
Nadezdna
N
Elizarova
Elvira
E
Melentyey
Alexander
A
Mutovkin
Ebgeny
E
Zhakharova
Svetlana
S
Fedorin
Ivan
I
D000995:Antitubercular Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002681:China; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D006801:Humans; D008297:Male; D018598:Minisatellite Repeats; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D011329:Prisoners; D012293:Rifampin / Q000494:pharmacology* / Q000627:therapeutic use; D012426:Russia / Q000453:epidemiology; D014376:Tuberculosis / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*
D000995:Antitubercular Agents; D012293:Rifampin