title: Proteomic study of non-typable Haemophilus influenzae.
abstract: Non-typable Haemophilus influenzae (NTHi) are small, gram-negative bacteria and are strictly human pathogens, causing acute otitis media, sinusitis and community-acquired pneumonia. There is no vaccine available for NTHi, as there is for H. influenzae type b. Recent advances in proteomic techniques are finding novel applications in the field of vaccinology. There are several protein separation techniques available today, each with inherent advantages and disadvantages. We employed a combined proteomics approach, including sequential extraction and analytical two-dimensional polyacrylamide electrophoresis (2D PAGE), and two-dimensional semi-preparative electrophoresis (2D PE), in order to study protein expression in the A4 NTHi strain. Although putative vaccine candidates were identified with both techniques, 11 of 15 proteins identified using the 2D PE approach were not identified by 2D PAGE, demonstrating the complementarily of the two methods.
authors.affiliation: Institute of Medical Biochemistry, Göteborg University, Box 440, Sweden.
authors.forename: Kajsa
authors.initials: K
authors.lastname: Thorén
authors.forename: Elisabet
authors.initials: E
authors.lastname: Gustafsson
authors.forename: Annica
authors.initials: A
authors.lastname: Clevnert
authors.forename: Thomas
authors.initials: T
authors.lastname: Larsson
authors.forename: Jörgen
authors.initials: J
authors.lastname: Bergström
authors.forename: Carol L
authors.initials: CL
authors.lastname: Nilsson
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D006193:Haemophilus influenzae / Q000737:chemistry*; D013058:Mass Spectrometry; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins