[Study on hydrolysis conditions of Dioscorea nipponica with uniform design method].
OBJECTIVE
To optimize hydrolysis conditions of Dioscorea nipponica so as to provide parameters for extracting diosgenin in industry.


METHODS
The optimum hydrolysis conditions were investigated using diosgenin as an index with uniform design and high performance liquid chromatography method.


RESULTS
The optimum conditions were as follows: the hydrochloric acid concentration was 2.5 mol/L, solid-liquid rate was 1:5, the reaction temperature was 140 degrees C, the reaction time was 4.0 h, the mix round speed was 120 r/min.


CONCLUSIONS
The content of diosgenin under the optimum hydrolysis conditions is raised, and the hydrochloric acid concentration should be strictly controlled because of its large influences on the results.
Wang
Jun
J
Jiangsu University, Zhenjiang 212013.
Yang
Kedi
K
Chen
Jun
J
D027742:Dioscorea / Q000737:chemistry*; D004144:Diosgenin / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification*; D006851:Hydrochloric Acid / Q000032:analysis; D006868:Hydrolysis; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D004144:Diosgenin; D006851:Hydrochloric Acid