title: High-performance liquid chromatography coupled with negative ion tandem mass spectrometry for determination of pravastatin in human plasma.
abstract: A new method, using high-performance liquid chromatography/ion electrospray (negative ion) mass spectrometry, has been developed for the determination of a hydrophilic liver-specific inhibitor of the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase, pravastatin in human plasma. In this method, plasma samples were prepared by a solid-phase extraction on C(18) Bond Elut cartridge. Chromatography was carried out with a Zorbax C(8) column. Simple isocratic chromatography conditions were used. The method has been validated in a linear range of 0.25-300 ng/ml with a coefficient of variation of 0.6-3.4%. The overall recovery was 90.5% for pravastatin and 90.8% for the internal standard beta-hydroxy-lovastatin. The method is simple and reliable with a total run time of less than 2 min.
authors.affiliation: Pharmascience, 6111 Royalmount Ave., Suite 100, Quebec, H4P 2T4, Montreal, Canada. zzhu@pharmascience.com
authors.forename: Zhimeng
authors.initials: Z
authors.lastname: Zhu
authors.forename: Len
authors.initials: L
authors.lastname: Neirinck
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000097:blood*; D013058:Mass Spectrometry / Q000379:methods*; D017035:Pravastatin / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D017035:Pravastatin