AMP kinase is required for mitochondrial biogenesis in skeletal muscle in response to chronic energy deprivation.
Mitochondrial biogenesis is a critical adaptation to chronic energy deprivation, yet the signaling mechanisms responsible for this response are poorly understood. To examine the role of AMP-activated protein kinase (AMPK), an evolutionarily conserved fuel sensor, in mitochondrial biogenesis we studied transgenic mice expressing a dominant-negative mutant of AMPK in muscle (DN-AMPK). Both DN-AMPK and WT mice were treated with beta-guanidinopropionic acid (GPA), a creatine analog, which led to similar reductions in the intramuscular ATPAMP ratio and phosphocreatine concentrations. In WT mice, GPA treatment resulted in activation of muscle AMPK and mitochondrial biogenesis. However, the same GPA treatment in DN-AMPK mice had no effect on AMPK activity or mitochondrial content. Furthermore, AMPK inactivation abrogated GPA-induced increases in the expression of peroxisome proliferator-activated receptor gamma coactivator 1alpha and calciumcalmodulin-dependent protein kinase IV (both master regulators of mitochondrial biogenesis). These data demonstrate that by sensing the energy status of the muscle cell, AMPK is a critical regulator involved in initiating mitochondrial biogenesis.
Zong
Haihong
H
Howard Hughes Medical Institute and the Departments of Internal Medicine, Cell Biology, and Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510, USA.
Ren
Jian Ming
JM
Young
Lawrence H
LH
Pypaert
Marc
M
Mu
James
J
Birnbaum
Morris J
MJ
Shulman
Gerald I
GI
D000227:Adenine Nucleotides / Q000378:metabolism; D000263:Adenylate Kinase / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000096:biosynthesis / Q000235:genetics; D004734:Energy Metabolism / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D005799:Genes, Dominant; D006146:Guanidines / Q000494:pharmacology; D051379:Mice; D008822:Mice, Transgenic; D008931:Mitochondria, Muscle / Q000502:physiology*; D009124:Muscle Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D010725:Phosphocreatine / Q000378:metabolism; D011422:Propionates / Q000494:pharmacology; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics
D000227:Adenine Nucleotides; D006146:Guanidines; D009124:Muscle Proteins; D011422:Propionates; D014157:Transcription Factors; C111264:peroxisome-proliferator-activated receptor-gamma coactivator-1; D010725:Phosphocreatine; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C517685:Camk4 protein, mouse; D000263:Adenylate Kinase; C005775:guanidinopropionic acid