Cyclosporin A as a model antigen: immunochemical and structural studies.
The immunosuppressant drug cyclosporin (Cs) A is a cyclic undecapeptide which has been used as a model antigen because structural information and a large number of analogs, modified at each of its 11 positions, were available. This review summarizes immunochemical and crystallographic studies of the interaction between the Fab of monoclonal antibody R45-45-11 and Cs. Three points are discussed: (1) the different conformations of CsA and the question of its biologically active form; (2) the Fab-CsA recognition mechanism; and (3) the relationship between structure and binding properties of CsA analogs.
Altschuh
Danièle
D
UMR 7100 - CNRS/ULP 'Biotechnologie des Interactions Moléculaires', Ecole Supérieure de Biotechnologie de Strasbourg, Pôle API, Bld Sébastien Brandt, 67400 Illkirch, France. daniele.altschuh@esbs.u-strasbg.fr
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism; D000941:Antigens / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D016572:Cyclosporine / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D007120:Immunochemistry; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000378:metabolism; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D018448:Models, Immunological; D008958:Models, Molecular; D015394:Molecular Structure; D011485:Protein Binding; D011487:Protein Conformation; D055672:Static Electricity
D000911:Antibodies, Monoclonal; D000941:Antigens; D007140:Immunoglobulin Fab Fragments; D007166:Immunosuppressive Agents; D046911:Macromolecular Substances; D016572:Cyclosporine