Structural and serological characterization of the lipopolysaccharide from Proteus penneri 20 and classification of the cross-reacting Proteus penneri strains 10, 16, 18, 20, 32 and 45 in Proteus serogroup O17.
O-specific polysaccharide (O-antigen) of the lipopolysaccharide of Proteus penneri 20 was studied using sugar analysis along with various one- and two-dimensional NMR spectroscopy techniques. The following structure of the polysaccharide was established: [formula: see text] It has the same carbohydrate backbone structure as that described earlier for P. penneri 16, in which the positions of the O-acetyl groups have not been determined. P. penneri 20 O-antiserum showed a strong cross-reactivity with the lipopolysaccharides of P. penneri 10, 16, 18, 32, 45 and P. mirabilis O17. These data enable classifying these strains together with P. penneri 20 in one Proteus serogroup, O17.
Sidorczyk
Zygmunt
Z
Department of General Microbiology, Institute of Microbiology and Immunology, University of Lódź, Banacha 12/16, 90-237 Lódź, Poland. zsidor@biol.uni.lodz.pl
Toukach
Filip V
FV
Zych
Krystyna
K
Arbatsky
Nikolay P
NP
Drzewiecka
Dominika
D
Ziółkowski
Andrzej
A
Shashkov
Alexander S
AS
Knirel
Yuriy A
YA
D000107:Acetylation; D000818:Animals; D000907:Antibodies, Bacterial; D015373:Bacterial Typing Techniques; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D006801:Humans; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D011511:Proteus / Q000145:classification* / Q000276:immunology* / Q000472:pathogenicity; D011817:Rabbits; D012703:Serotyping; D013045:Species Specificity
D000907:Antibodies, Bacterial; D019081:O Antigens