Application of directly coupled high performance liquid chromatography-NMR-mass spectometry and 1H NMR spectroscopic studies to the investigation of 2,3-benzofuran metabolism in Sprague-Dawley rats.
The urinary excretion of metabolites of 2,3-benzofuran was studied in Sprague-Dawley rats (n = 5) given a single dose of 150 mg/kg i.p. Urine samples were collected at defined intervals up to 7 days postdose and analyzed using (1). H NMR and directly coupled high performance liquid chromatography (HPLC)-NMR, HPLC-(mass spectrometry) MS and HPLC-MS-NMR methods. The principal metabolites were determined to be 2-hydroxyphenylacetic acid and 2-(2-hydroxyethyl)phenyl hydrogen sulfate, representing 24.3 +/- 6.0% and 19.6 +/- 6.4% of the dose, respectively. This indicates that metabolism of benzofuran to the polar species excreted in urine involves cleavage of the furan ring.
Connelly
John C
JC
Department of Biological Chemistry, Faculty of Medicine, Imperial College of Science, Technology, and Medicine, London, United Kingdom.
Connor
Susan C
SC
Monte
Soria
S
Bailey
Nigel J C
NJ
Borgeaud
Nathan
N
Holmes
Elaine
E
Troke
Jeff
J
Nicholson
Jeremy K
JK
Gavaghan
Claire L
CL
D000818:Animals; D001572:Benzofurans / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley
D001572:Benzofurans; C105430:benzofuran