In vivo plasmid DNA electrotransfer.
In vivo electrotransfer is a physical technique for gene delivery in various mammalian tissues, which involves the injection of plasmid DNA into a target tissue and administration of an electric field. Its ease of performance, as well as recent understanding of its mechanism and applications to different mammalian tissues such as skeletal muscle, liver, brain and tumors, makes it a powerful technique. It could be used in gene therapy and as a laboratory tool to study gene functions.
Bigey
Pascal
P
Laboratoire de Chimie Bioorganique et de Biotechnologie Moléculaire et Cellulaire, UMR 7001 ENSCP/CNRS/Aventis, CRVA-Aventis, Batiment Monod, Laboratory 3C05, 13 quai Jules Guesde, 94403 Vitry-sur-Seine cedex, France.
Bureau
Michel F
MF
Scherman
Daniel
D
D000818:Animals; D004247:DNA; D004574:Electromagnetic Fields; D004586:Electrophoresis / Q000379:methods; D018274:Electroporation / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D018482:Muscle, Skeletal / Q000502:physiology; D009369:Neoplasms / Q000628:therapy*; D010957:Plasmids / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D004247:DNA