title: High-performance liquid chromatographic analysis of chrysin derivatives on a Nova-Pak C18 column.
abstract: A high-performance liquid chromatographic method has been developed for the separation and quantification of chrysin and synthetic chrysin derivatives (12 chrysin alkyl and 7 chrysin acyl derivatives). The chromatography was performed using a Nova-Pak C18 column. A RP-HPLC was performed by using a binary mixture (MeOH-10 mM H3PO4) as a mobile phase, and the column temperature was maintained at room temperature. A flow rate was 1.0 ml/min, and the effluent was monitored at a wavelenth of 280 nm. The retention times for chrysin acyl and alkyl derivatives were within 10 minutes and 20 minutes, respectively. The absolute recovery of samples were all over 96%. The detection limits were 0.1-18 ng at S/N = 3 ratio.
authors.affiliation: College of Pharmacy, Seoul National University, Kwanak-Gu, Korea.
authors.forename: Kyoung Soon
authors.initials: KS
authors.lastname: Kim
authors.forename: Joon Su
authors.initials: JS
authors.lastname: Shin
authors.forename: Youmie
authors.initials: Y
authors.lastname: Park
authors.forename: Sanghyun
authors.initials: S
authors.lastname: Lee
authors.forename: Yang Bae
authors.initials: YB
authors.lastname: Kim
authors.forename: Bak-Kwang
authors.initials: BK
authors.lastname: Kim
mesh_terms: D002250:Carbon Radioisotopes / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry*
chemical_list: D002250:Carbon Radioisotopes; D005419:Flavonoids; C043561:chrysin