title: Inhibitory effect against polymerase and ribonuclease activities of HIV-reverse transcriptase of the aqueous leaf extract of Terminalia triflora.
abstract: Dichloromethane, methanol and aqueous extracts from the leaves of Terminalia triflora were investigated for their inhibitory effect on polymerase and ribonuclease activities of HIV reverse transcriptase.The most potent activity was found in the aqueous extract, which inhibited both polymerase and ribonuclease activities of the enzyme with an IC50 of 1.6 micro g/mL and 1.8 micro g/mL respectively. The antiinfective activity of the extract was demonstrated in HLT4LacZ-IIIB cell culture with an IC50 of 1.0 micro g/mL. The extract was submitted to a purification process by extractive and chromatographic methods. The activity remained in the hydrophillic fraction. Tannins present in this active purified fraction, as determined by TLC and HPLC methods, could account for the anti HIV-RT activity found in the aqueous extract.
authors.affiliation: Instituto de Química y Metabolismo del Fármaco (IQUIMEFA-PROSIVAD) (UBA-CONICET), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, República Argentina.
authors.forename: V S
authors.initials: VS
authors.lastname: Martino
authors.forename: P
authors.initials: P
authors.lastname: López
authors.forename: J J
authors.initials: JJ
authors.lastname: Martinez Irujo
authors.forename: M
authors.initials: M
authors.lastname: Sanromán
authors.forename: M T
authors.initials: MT
authors.lastname: Cuevas
authors.forename: E
authors.initials: E
authors.lastname: Santiago
authors.forename: J J
authors.initials: JJ
authors.lastname: Lasarte
authors.forename: M
authors.initials: M
authors.lastname: Font
authors.forename: J D
authors.initials: JD
authors.lastname: Coussio
authors.forename: A
authors.initials: A
authors.lastname: Monge
mesh_terms: D019380:Anti-HIV Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002460:Cell Line / Q000187:drug effects / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D015658:HIV Infections / Q000188:drug therapy; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D015497:HIV-1 / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D020128:Inhibitory Concentration 50; D019384:Nucleic Acid Synthesis Inhibitors; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D018894:Reverse Transcriptase Inhibitors / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D012260:Ribonucleases / Q000187:drug effects; D029766:Terminalia
chemical_list: D019380:Anti-HIV Agents; D019384:Nucleic Acid Synthesis Inhibitors; D010936:Plant Extracts; D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase; D012260:Ribonucleases