Characterization of a holin (HolNU3-1) in methicillin-resistant Staphylococcus aureus host.
The gene encoding holin protein HolNU3-1 from a clinical isolate of methicillin-resistant Staphylococcus aureus (MRSA) NU3-1 was cloned and expressed in S. aureus RN4220. HolNU3-1 encoded by the holNU3-1 gene, which is located upstream of the deleted endolysin gene, was functional. Expression of the holNU3-1 gene induced a decrease in culture turbidity and formation of translucent (empty ghost) cells in S. aureus. We found heterogeneity of the holin genes and diversity of the two-component lysis system, which consists of holin and endolysin, in MRSA hosts. We suggest that this diversity is important in the identification of the evolution of clinical isolates of S. aureus.
Horii
Toshinobu
T
Department of Laboratory Medicine and Group of Infection Control Research, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Hamamatsu 431-3192, Japan. horiihm@hama-med.ac.jp
Suzuki
Yasuhiro
Y
Kobayashi
Miya
M
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001435:Bacteriophages; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D006801:Humans; D016106:Methicillin Resistance; D008969:Molecular Sequence Data; D009238:N-Acetylmuramoyl-L-alanine Amidase; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; C108616:LytH protein, Staphylococcus aureus; D009238:N-Acetylmuramoyl-L-alanine Amidase