title: Local expression of tumor necrosis factor-alpha and interleukin-10 on wound healing of intestinal anastomosis during endotoxemia in mice.
abstract: BACKGROUND
  This study aimed to evaluate the integrity of anastomotic wound healing after digestive surgery under septic conditions and define the participation of local expression of tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) around the anastomotic segment.
  
  
  METHODS
  Experimental animals were divided into lipopolysaccharide (LPS) and control groups, which had either LPS or normal saline solution injected into the peritoneal cavity 24 h before transection and anastomosis of the colon. Anastomotic bursting pressure (ABP) and tissue hydroxyproline concentration (HP) were measured as indicators of wound healing. Immunohistochemical staining for TNF-alpha and IL-10 on tissue samples obtained from the anastomotic segment were examined 1, 6, and 24 h after the operation. The reactive cells were counted under light microscopy.
  
  
  RESULTS
  ABP and HP were significantly lower in the LPS group than in the control group 7 days after surgery. In the LPS group, TNF-alpha expression increased about threefold over that in the control group 1 h after the operation. TNF-alpha-reactive cells were observed until 24 h after the operation in the LPS group, but not in the control group. On the other hand, IL-10 was not expressed in the control group during the observed period, whereas IL-10 was observed 24 h after the operation in the LPS group.
  
  
  CONCLUSIONS
  It is suggested that anastomotic wound healing was impaired after the digestive surgery in animals treated with intraperitoneal LPS, and that local expression of TNF-alpha and IL-10 at the anastomotic site acts as an inhibitory factor in the wound healing process.
authors.affiliation: First Department of Surgery, Kagawa Medical University, 1750-1, Ikenobe, Miki, Kita, Kagawa, 761-0793, Japan. ishimura@kms.ac.jp
authors.forename: Ken
authors.initials: K
authors.lastname: Ishimura
authors.forename: Akito
authors.initials: A
authors.lastname: Moroguchi
authors.forename: Keiichi
authors.initials: K
authors.lastname: Okano
authors.forename: Takashi
authors.initials: T
authors.lastname: Maeba
authors.forename: Hajime
authors.initials: H
authors.lastname: Maeta
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D019446:Endotoxemia / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D006909:Hydroxyproline / Q000032:analysis; D007150:Immunohistochemistry; D016753:Interleukin-10 / Q000032:analysis*; D007422:Intestines / Q000737:chemistry* / Q000473:pathology / Q000601:surgery; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011312:Pressure; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D006909:Hydroxyproline