title: Stability of fibrin sealant in cerebrospinal fluid: an in vitro study.
abstract: OBJECTIVE
  The in vitro stability of fibrin sealant in cerebrospinal fluid (CSF) was investigated to verify the efficacy of intracranial application of fibrin sealant.
  
  
  METHODS
  Human CSF was collected from 11 patients. Fibrin glue spheres (diameter, 5 mm) were incubated in CSF specimens at 37 degrees C. At 2, 4, 8, 24, and 48 hours, the diameter of the spheres was measured and the ultrastructure was evaluated by transmission electron microscopy. A control group consisted of sealant spheres in physiological saline (n = 2).
  
  
  RESULTS
  In all CSF samples, the fibrin sealant did not degrade with time. The size, consistency, color, and shape of the sealant remained unchanged, even 48 hours after placement in the CSF. Transmission electron microscopic analysis of the fibrin sealant revealed an amorphous, fibrinous meshwork. No morphological differences existed between fibrin sealant complex placed in the CSF for different time periods and sealant placed in physiological saline.
  
  
  CONCLUSIONS
  Within the limitations of this in vitro study, human CSF has no adverse effects on fibrin sealant in terms of alteration of structure and morphology. Fibrin sealant is stable in a CSF environment and can be effectively used in the cisternal or subarachnoidal space.
authors.affiliation: Department of Neurosurgery, University Medical Center St. Radboud, Nijmegen, The Netherlands.
authors.forename: Tomas
authors.initials: T
authors.lastname: Menovsky
authors.forename: Joost
authors.initials: J
authors.lastname: de Vries
authors.forename: Marius
authors.initials: M
authors.lastname: van den Bergh Weerman
authors.forename: J André
authors.initials: JA
authors.lastname: Grotenhuis
mesh_terms: D000328:Adult; D000368:Aged; D002675:Child, Preschool; D004355:Drug Stability; D005260:Female; D015718:Fibrin Tissue Adhesive / Q000134:cerebrospinal fluid* / Q000737:chemistry*; D006801:Humans; D007223:Infant; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D014014:Tissue Adhesives / Q000737:chemistry* / Q000493:pharmacokinetics*
chemical_list: D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives