title: Enhancement of nestin protein-immunoreactivity induced by ionizing radiation in the forebrain ependymal regions of rats.
abstract: Expression of nestin was immunohistochemically examined in the forebrains of rats receiving ionizing radiation. Nestin-immunoreactive cells were predominately distributed in ependymal regions. Nestin-immunoreactivity in ependymal regions of irradiated rats increased significantly from 1 to 4 weeks after ionizing radiation compared with that of controls. Double immunofluorescence confirmed that about 94% of nestin-positive cells exhibited glial fibrillary acidic protein-immunoreactivity and a minor population of them showed Ki-67-immunoreactivity in these regions. The results have provided evidence for up-regulation of nestin expression induced by ionizing radiation in ependymal cells, suggesting that these reactive ependymal cells may be involved in remodeling and repairing processes of brain irradiation injury.
authors.affiliation: Department of Radiotherapy, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Shi
authors.forename: Li-Chun
authors.initials: LC
authors.lastname: Wei
authors.forename: Rong
authors.initials: R
authors.lastname: Cao
authors.forename: Liang-Wei
authors.initials: LW
authors.lastname: Chen
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000378:metabolism / Q000503:physiopathology; D002552:Cerebral Ventricles / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D004805:Ependyma / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism / Q000528:radiation effects; D007381:Intermediate Filament Proteins / Q000378:metabolism* / Q000528:radiation effects; D019394:Ki-67 Antigen / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology* / Q000528:radiation effects; D009419:Nerve Tissue Proteins; D064231:Nestin; D016548:Prosencephalon / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D011839:Radiation, Ionizing; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D015854:Up-Regulation / Q000502:physiology* / Q000528:radiation effects
chemical_list: D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D019394:Ki-67 Antigen; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin