[Pseudomonas aeruginosa vaccine on the basis of antigens isolated from the supernatant of culture media K-4].
The possibility of using experimental culture medium K-4 prepared on the basis of casein hydrolysate peptides with the isoelectric point 4.1 for obtaining antigens from P. aeruginosa strains was evaluated. Two antigenic fractions were isolated from the culture fluid containing extracellular slime. The study of the toxicity of the antigenic preparations revealed that one of these fractions had low toxicity for mice (the second antigenic fraction was highly toxic). The former P. aeruginosa antigenic fraction was used for obtaining pyocyanic vaccine. One vaccination dose of this vaccine contained 0.2 mg of the antigen adsorbed on aluminum hydroxide. Pyocyanic vaccine ensured the active protection of mice challenged with P. aeruginosa homologous and heterologous strains.
Nuriddinova
N R
NR
Research Institute for Vaccines and Sera, Tashkent, Uzbekistan.
Ivanova
L E
LE
Sheremet'ev
N N
NN
Garib
F Iu
FIu
D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage* / Q000302:isolation & purification / Q000633:toxicity; D002498:Centrifugation; D011232:Chemical Precipitation; D003470:Culture Media / Q000737:chemistry*; D003956:Dialysis; D004195:Disease Models, Animal; D019811:Hydroxylamine; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D051379:Mice; D011552:Pseudomonas Infections / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000276:immunology*; D014611:Vaccination; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000302:isolation & purification / Q000633:toxicity
D000942:Antigens, Bacterial; D003470:Culture Media; D022223:Vaccines, Subunit; D019811:Hydroxylamine