title: Tryptophans 286 and 288 in the C-terminal region of protein B23.1 are important for its nucleolar localization.
abstract: Nucleolar protein B23 can shuttle between the nucleolus and cytoplasm. However, the mechanism involved in the protein moving and staying in the nucleolus is not fully understood. To identify the nucleolar localization signal sequence of protein B23, we examined the subnuclear location of B23.1 mutant proteins fused with green fluorescent protein in HeLa cells. The results suggested that the two C-terminal tryptophan residues (Trp-286 and Trp-288) of protein B23.1 were important in this phenomenon.
authors.affiliation: Department of Sustainable Resource Science, Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu, Mie 514-8507, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohkubo
authors.forename: Yukio
authors.initials: Y
authors.lastname: Furuichi
authors.forename: Hayato
authors.initials: H
authors.lastname: Umekawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002466:Cell Nucleolus / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D049452:Green Fluorescent Proteins; D007536:Isomerism; D008164:Luminescent Proteins / Q000737:chemistry; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry* / Q000648:ultrastructure; D000090243:Nucleophosmin; D017354:Point Mutation; D011233:Precipitin Tests; D051381:Rats; D014364:Tryptophan / Q000737:chemistry*
chemical_list: D008164:Luminescent Proteins; D009687:Nuclear Proteins; D000090243:Nucleophosmin; D049452:Green Fluorescent Proteins; D014364:Tryptophan