Separation of biosynthetic polyunsaturated fatty acid (PUFA) with supercritical fluid.
The separation of biosynthetic polyunsaturated fatty acid (PUFA) was undertaken with supercritical fluid. The polyunsaturated fatty acid was synthesised by WL-1021, which is a strain of marine bacterium isolated from marine fish. The polyunsaturated fatty acid can be very efficiently extracted from WL-1021 that has the characteristics of rapid growth in the artificial medium. The PUFA in WL-1021 has been successfully separated by supercritical fluid extraction (SFE). The SFE process parameters including pressure, temperature and culture temperature variations have been measured by High Performance Liquid Chromatography (HPLC), GC/FT-IR and GC/MS.
Wang
Li
L
Department of Environmental Engineering, College of Resources of Civil Engineering, Northeastern University, Shenyang, China, Postcode: 110006. liwang@uow.edu.au
Muttucumaru
Sivakumar
S
D000818:Animals; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D019149:Bioreactors; D002245:Carbon Dioxide / Q000378:metabolism; D002478:Cells, Cultured; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology; D004281:Docosahexaenoic Acids / Q000302:isolation & purification / Q000378:metabolism; D015118:Eicosapentaenoic Acid; D005231:Fatty Acids, Unsaturated / Q000302:isolation & purification* / Q000378:metabolism; D005399:Fishes / Q000382:microbiology; D008386:Marine Biology / Q000379:methods; D008954:Models, Biological; D011312:Pressure; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013269:Stochastic Processes; D013696:Temperature; D014871:Water Microbiology
D003470:Culture Media; D005231:Fatty Acids, Unsaturated; D002245:Carbon Dioxide; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid