Nasal immunization with homogenate and peptide antigens induces protective immunity against Trichinella spiralis.
Mice were successfully immunized against the intestinal nematode Trichinella spiralis by intranasal administration of a 30-mer peptide antigen with cholera toxin B. Immunized mice developed antigen-specific serum immunoglobulin G1, intestinal immunoglobulin A, and a type 2-biased cytokine response. Intranasal immunization therefore generates the Th2-mediated responses required for immunity against intestinal parasites.
McGuire
Carolann
C
School of Life and Environmental Sciences, University of Nottingham, University Park, United Kingdom.
Chan
Weng C
WC
Wakelin
Derek
D
D000281:Administration, Intranasal; D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D002772:Cholera Toxin / Q000235:genetics / Q000276:immunology; D005260:Female; D007114:Immunization; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000517:prevention & control*; D014614:Vaccines, Synthetic / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D010455:Peptides; D014614:Vaccines, Synthetic; D002772:Cholera Toxin