title: Definitive high resolution typing of HLA-E allelic polymorphisms: Identifying potential errors in existing allele data.
abstract: A set of robust PCR-SSP reactions were developed for each of the five polymorphic sites that define the five alleles of the HLA class Ib gene, HLA-E. This method was developed using 28 homozygous cell lines and further tested in a sample of African-Americans, a sample of Japanese, and a core panel of cell lines compiled for the 13th International Histocompatibility Workshop. Three alleles were found in each of these four sample groups, HLA-E*0101 (64.29, 50.00, 32.00 and 56.58%, respectively), *01031 (5.36, 20.65, 39.00 and 18.42%) and *01032 (30.35, 29.35, 29.00, and 25.00%). HLA-E*0102 was not detected in any of these samples nor in the cell line, LCL 722.221, in which this allele was originally described. HLA-E*0104 was not found either. This latter allele was originally reported in Japanese at a frequency of 1/22 (4.5%), which should have been high enough to have resulted in multiple occurrences of the *0104 allele in the samples tested in this study. We propose that the existence of the HLA-E*0102 and E*0104 alleles should be questioned.
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
authors.forename: C
authors.initials: C
authors.lastname: Grimsley
authors.forename: A
authors.initials: A
authors.lastname: Kawasaki
authors.forename: C
authors.initials: C
authors.lastname: Gassner
authors.forename: N
authors.initials: N
authors.lastname: Sageshima
authors.forename: Y
authors.initials: Y
authors.lastname: Nose
authors.forename: K
authors.initials: K
authors.lastname: Hatake
authors.forename: D E
authors.initials: DE
authors.lastname: Geraghty
authors.forename: A
authors.initials: A
authors.lastname: Ishitani
mesh_terms: D000483:Alleles; D006680:HLA Antigens / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods* / Q000592:standards*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods / Q000592:standards; D000097625:HLA-E Antigens
chemical_list: D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D000097625:HLA-E Antigens