Arachidonic acid stimulates internalisation of leptin by human placental choriocarcinoma (BeWo) cells.
Arachidonic acid at 100 nM stimulated internalisation of 125I-leptin in human placental choriocarcinoma (BeWo) cells by 3-fold compared with controls. In contrast, eicosapentaenoic acid at similar concentration decreased internalisation of leptin by 2-fold. Use of ibuprofen and indomethacin (inhibitors of prostaglandin synthesis) inhibited the stimulatory effect of arachidonic acid. Prostaglandin E(2), a cyclooxygenase metabolite of arachidonic acid, stimulated internalisation of leptin by these cells. All these data demonstrate that stimulation of leptin internalisation by arachidonic acid in placental trophoblasts may be mediated via prostaglandin E(2).
Duttaroy
Asim K
AK
Institute for Nutrition Research, University of Oslo, P.O. Box 1046, Blindern, N-0316, Oslo, Norway. a.k.d@basalmed.uio.no
Taylor
Jonathon
J
Gordon
Margaret J
MJ
Hoggard
Nigel
N
Campbell
Fiona M
FM
D016718:Arachidonic Acid / Q000378:metabolism*; D001692:Biological Transport / Q000502:physiology*; D002822:Choriocarcinoma / Q000378:metabolism; D005227:Fatty Acids / Q000494:pharmacology; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes / Q000378:metabolism; D020738:Leptin / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy; D011453:Prostaglandins / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000378:metabolism; D054411:Receptors, Leptin; D014327:Trophoblasts / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D005227:Fatty Acids; D007457:Iodine Radioisotopes; C515448:LEPR protein, human; D020738:Leptin; D011453:Prostaglandins; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D016718:Arachidonic Acid