Recombinant human growth hormone modulates the hepatic acute-phase response and P-selectin in burned rats.
The purpose of this study was to determine the effect of recombinant human growth hormone (rhGH) on serum constitutive proteins, cytokines, P-selectin, and insulin-like growth factor (IGF-1) in the thermally injured rats.Sprague-Dawley rats (64 males) were given 30% total body surface area full thickness scald burn. They were randomly divided to receive either 2.5mg/kg per day im rhGH or saline (control). Rats were sacrified on postburn days 1, 2, 5, and 7, and serum constitutive proteins, cytokines, P-selectin, and IGF-1 levels were measured.Serum IGF-1 levels were increased on days 2, 5, or 7 after burn in rhGH-treated rats compared with controls (P<0.001, <0.01 and <0.001, respectively). Serum transferrin and albumin levels were increased on days 7 after burn in rhGH-treated rats compared with controls (P<0.05). The cytokines increased after thermal injury. The rhGH decreased serum levels of tumor necrosis factor-alpha on postburn days 1 compared with controls (P<0.001). Serum levels of interleukin-1 were decreased on days 1 and 2 after burn in rhGH treated rats compared with controls (P<0.001, <0.01, respectively). Rats receiving rhGH showed significantly increased P-selectin levels at 5 and 7 postburn days compared with controls (P<0.001). Our data indicate that rhGH, given after thermal injury, increased albumin, transferrin, IGF-1, and P-selectin levels and decreased serum tumor necrosis factor-alpha and interleukin-1 levels.
Basoglu
Mahmut
M
Department of General Surgery, School of Medicine, Ataturk University, Erzurum, Turkey. mabasoglu@hotmail.com
Kiziltunc
Ahmet
A
Yildirgan
M Ilhan
MI
Gumustekin
Kenan
K
Gumus
Metehan
M
Yildirim
Abdulkadir
A
Atamanalp
S Selcuk
SS
D000210:Acute-Phase Reaction; D000818:Animals; D002056:Burns / Q000097:blood / Q000188:drug therapy* / Q000276:immunology; D019382:Human Growth Hormone / Q000627:therapeutic use*; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D007375:Interleukin-1 / Q000097:blood; D008099:Liver / Q000378:metabolism; D008297:Male; D019007:P-Selectin / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000032:analysis; D012867:Skin / Q000276:immunology / Q000293:injuries*; D013997:Time Factors; D014168:Transferrin / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D015430:Weight Gain
D007375:Interleukin-1; D019007:P-Selectin; D012709:Serum Albumin; D014168:Transferrin; D014409:Tumor Necrosis Factor-alpha; D019382:Human Growth Hormone; D007334:Insulin-Like Growth Factor I