Antisense down regulation of NtBI-1 in tobacco BY-2 cells induces accelerated cell death upon carbon starvation.
Bax inhibitor-1 (BI-1) protein is proposed to be a conserved programmed cell death suppressor. In this report, we investigate the anti-apoptotic function of plant BI-1 by antisense (AS) down regulation of NtBI-1 in Nicotiana tabacum cv. BY-2 cells. We observed that AS cell lines were more susceptible to autophagy, internucleosomal DNA fragmentation and death than control cells when subjected to sucrose starvation and hypo-osmotic shock, in agreement with a role of BI-1 as a death inhibitor.
Bolduc
Nathalie
N
Department of Biochemistry and Microbiology, Life and Health Science Pavilion, Laval University, Quebec, QC, Canada G1K 7P4.
Brisson
Louise F
LF
D017209:Apoptosis; D002460:Cell Line; D053938:DNA Fragmentation; D016373:DNA, Antisense / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis; D015536:Down-Regulation; D007700:Kinetics; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D013395:Sucrose / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology* / Q000254:growth & development
D016373:DNA, Antisense; D018744:DNA, Plant; D008565:Membrane Proteins; D013395:Sucrose