title: [Study on hydrolysis conditions of Dioscorea nipponica with uniform design method].
abstract: OBJECTIVE
  To optimize hydrolysis conditions of Dioscorea nipponica so as to provide parameters for extracting diosgenin in industry.
  
  
  METHODS
  The optimum hydrolysis conditions were investigated using diosgenin as an index with uniform design and high performance liquid chromatography method.
  
  
  RESULTS
  The optimum conditions were as follows: the hydrochloric acid concentration was 2.5 mol/L, solid-liquid rate was 1:5, the reaction temperature was 140 degrees C, the reaction time was 4.0 h, the mix round speed was 120 r/min.
  
  
  CONCLUSIONS
  The content of diosgenin under the optimum hydrolysis conditions is raised, and the hydrochloric acid concentration should be strictly controlled because of its large influences on the results.
authors.affiliation: Jiangsu University, Zhenjiang 212013.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Kedi
authors.initials: K
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
mesh_terms: D027742:Dioscorea / Q000737:chemistry*; D004144:Diosgenin / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification*; D006851:Hydrochloric Acid / Q000032:analysis; D006868:Hydrolysis; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D004144:Diosgenin; D006851:Hydrochloric Acid