title: Up-regulation of the human serum and glucocorticoid-dependent kinase 1 in glomerulonephritis.
abstract: Glomerulonephritis is paralleled by excessive formation of transforming growth factor-beta (TGF-beta), which participates in the pathophysiology of the disease. Recently, a novel downstream target of TGF-beta has been identified, i.e. the human serum and glucocorticoid-dependent kinase 1 (hSGK1), a serine/threonine kinase participating in the regulation of Na(+) transport. The present study was performed to elucidate transcriptional regulation of hSGK1 in glomerulonephritis. To this end, in situ hybridization was performed in biopsies from patients with clinical diagnosis of glomerulonephritis. hSGK1 transcript levels were moderately enhanced in 5 out of 9 patients and strongly enhanced in 4 out of 9 patients. Distal nephron epithelial cell hSGK1 transcript levels were low or absent in 7 of the 9 patients but markedly enhanced in 2 of the 9 patients. In conclusion, glomerulonephritis leads to glomerular and in some cases to epithelial up-regulation of hSGK1 transcription.
authors.affiliation: Department of Physiology, University of Tübingen, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Friedrich
authors.forename: S
authors.initials: S
authors.lastname: Wärntges
authors.forename: K
authors.initials: K
authors.lastname: Klingel
authors.forename: M
authors.initials: M
authors.lastname: Sauter
authors.forename: R
authors.initials: R
authors.lastname: Kandolf
authors.forename: T
authors.initials: T
authors.lastname: Risler
authors.forename: G A
authors.initials: GA
authors.lastname: Müller
authors.forename: R
authors.initials: R
authors.lastname: Witzgall
authors.forename: W
authors.initials: W
authors.lastname: Kriz
authors.forename: H J
authors.initials: HJ
authors.lastname: Gröne
authors.forename: F
authors.initials: F
authors.lastname: Lang
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005921:Glomerulonephritis / Q000378:metabolism*; D006801:Humans; D017874:Immediate-Early Proteins; D017403:In Situ Hybridization; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014018:Tissue Distribution; D015854:Up-Regulation; D000099184:Serum-Glucocorticoid Regulated Kinases
chemical_list: D017874:Immediate-Early Proteins; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D000099184:Serum-Glucocorticoid Regulated Kinases