title: Expression of a novel member of sorting nexin gene family, SNX-L, in human liver development.
abstract: The sorting nexin (SNX) protein family is implicated in the regulation of receptor degradation and membrane traffic in the cell. With the aim of identifying novel genes involved in receptor degradation and recycling, we have cloned a new member of the sorting nexin gene family, human sorting nexin L, SNX-L (or SNX21). This gene includes 4 exons and 3 introns, and is located on chromosome 20q12-13.1 region, encompassing 8 kb. The full-length cDNA of SNX-L is 1,811 bp, with an open reading frame of 1,092 bp. The protein consists of 364 amino acids and encodes a 40 kDa protein. The SNX-L protein has a common PX domain shared by all SNX family members. The similarity of SNX-L PX domain to the PX consensus sequence is over 40%. PX domains have been shown to associate with specific phospholipids and membrane compartments. Expression analysis of SNX-L mRNA indicates that SNX-L is distinctly and highly expressed in fetus liver, but only weakly expressed in brain, muscle (skeleton muscle, smooth muscle, and cardiac muscle), kidney, and adrenal gland. Strong liver expression of SNX-L is maintained from 12 to 25 weeks during human fetus development, suggesting that SNX-L may be a regulatory gene involved in receptor protein degradation during embryonic liver development.
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha, 410081 Hunan, People's Republic of China.
authors.forename: Weiqi
authors.initials: W
authors.lastname: Zeng
authors.forename: Wuzhou
authors.initials: W
authors.lastname: Yuan
authors.forename: Yuequn
authors.initials: Y
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Jiao
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Chunxia
authors.initials: C
authors.lastname: Huang
authors.forename: Dali
authors.initials: D
authors.lastname: Li
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Li
authors.forename: Chuanbing
authors.initials: C
authors.lastname: Zhu
authors.forename: Xiushan
authors.initials: X
authors.lastname: Wu
authors.forename: Mingyao
authors.initials: M
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D005091:Exons; D005333:Fetus / Q000033:anatomy & histology / Q000502:physiology; D006801:Humans; D007438:Introns; D008099:Liver / Q000254:growth & development*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D058305:Sorting Nexins; D014018:Tissue Distribution; D033921:Vesicular Transport Proteins
chemical_list: D002352:Carrier Proteins; C470513:SNX21 protein, human; D058305:Sorting Nexins; D033921:Vesicular Transport Proteins