Characterization of the GO system of Pseudomonas aeruginosa.
The mutT, mutM, and mutY genes of the GO system of the Pseudomonas aeruginosa PAO1 strain have been characterized by cloning, sequencing, and complementation analysis. The three genes, when cloned in a plasmid, were able to complement the high mutation frequency of the corresponding Escherichia coli deficient strains. Our results demonstrate that the putative mutT, mutM, and mutY gene products from P. aeruginosa are able to perform the expected activity. In addition, the sequence of the P. aeruginosa mutT gene strongly suggested that the product of this gene has a bifunctional activity in P. aeruginosa, being the C-terminal part 40% identical to a consensus sequence of thiamine monophosphate synthases. Our results also demonstrated that the N-terminal part of the protein is necessary and sufficient for the 8-oxodGTP hydrolase activity.
Oliver
Antonio
A
Unidad de Microbiología Molecular, Servicio de Microbiología, Hospital Ramón y Cajal, Carretera de Colmenar Km 9.1, 28034, Madrid, Spain.
Sánchez
Juan Manuel
JM
Blázquez
Jesús
J
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D045647:DNA Glycosylases; D043382:DNA-Formamidopyrimidine Glycosylase; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005816:Genetic Complementation Test / Q000379:methods; D008969:Molecular Sequence Data; D009154:Mutation; D009699:N-Glycosyl Hydrolases / Q000235:genetics* / Q000502:physiology; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics* / Q000502:physiology; D016133:Polymerase Chain Reaction / Q000379:methods; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000378:metabolism; D011755:Pyrophosphatases; D011994:Recombinant Proteins / Q000032:analysis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D000080242:8-Hydroxy-2'-Deoxyguanosine; D010744:Phosphoric Monoester Hydrolases; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; C061738:mutY adenine glycosylase; D043382:DNA-Formamidopyrimidine Glycosylase; C468610:DNA-formamidopyrimidine glycosylase, E coli; D011755:Pyrophosphatases; C052211:mutT protein, E coli; D003849:Deoxyguanosine