title: Genetic variability in Puccinia striiformis f. sp. tritici populations sampled on a local scale during natural epidemics.
abstract: This study investigated genetic polymorphism on a local scale in Puccinia striiformis f. sp. tritici populations during natural epidemics, in four fields located in northern France and sampled in 1998 or 1999. Two hundred and forty-seven isolates were analyzed for their amplified fragment length polymorphism (AFLP) pattern through four primer combinations, and 194 of them were also tested for their virulence factors. Only one or two pathotypes were found in each field, and all isolates had virulence v17, matching the recently introduced Yr17 resistance gene. Polymorphism on a field scale was low. Although 67 loci were polymorphic, 77% of the isolates had the same AFLP pattern, all other patterns being rare or unique. Analyses of the genetic distance between AFLP patterns based on the Jaccard index allowed us to define 12 groups, but a bootstrap analysis showed that all isolates could be assigned to a single clonal lineage. This leads us to conclude that P. striiformis f. sp. tritici populations are clonal on a field scale in northern France.
authors.affiliation: Unité de Pathologie Végétale et Epidémiologie, INRA, 78850 Thiverval Grignon, France.
authors.forename: Lorys M M A
authors.initials: LM
authors.lastname: Villaréal
authors.forename: Christian
authors.initials: C
authors.lastname: Lannou
authors.forename: Claude
authors.initials: C
authors.lastname: De Vallavieille-Pope
authors.forename: Claire
authors.initials: C
authors.lastname: Neema
mesh_terms: D001487:Basidiomycota / Q000235:genetics* / Q000472:pathogenicity; D014644:Genetic Variation; D005838:Genotype; D021141:Nucleic Acid Amplification Techniques; D011110:Polymorphism, Genetic; D014774:Virulence