title: A new parvovirus genotype persistent in human skin.
abstract: Parvovirus B19 is the exclusive human pathogen of the Erythrovirus genus. In classical view, the B19 DNA sequence shows little variability, with no disease-specific or tissue type specific associations. We examined skin biopsies from patients with B19-unrelated skin disease or from constitutionally healthy adults by polymerase chain reaction assays for four different genomic regions of the B19 virus. Sequencing showed that the skin-derived viral DNA differed within the protein-coding region from the B19 reference sequences by 10.8% and from the V9 variant by 8.6% and within the noncoding region (covering nucleotides 189-435 of the promoter region) by 26.5 and 17.2%, respectively. Despite this sequence difference, the promoter region was shown by a luciferase gene expression assay to be biologically active. We have detected a new B19 virus genotype, K71, which differs extensively from the known B19-virus genotypes and is persistently carried in human skin.
authors.affiliation: Department of Virology, Haartman Institute and Helsinki University Central Hospital, University of Helsinki, FIN-00290 Helsinki, Finland. kati.hokynar@helsinki.fi
authors.forename: Kati
authors.initials: K
authors.lastname: Hokynar
authors.forename: Maria
authors.initials: M
authors.lastname: Söderlund-Venermo
authors.forename: Maria
authors.initials: M
authors.lastname: Pesonen
authors.forename: Annamari
authors.initials: A
authors.lastname: Ranki
authors.forename: Olli
authors.initials: O
authors.lastname: Kiviluoto
authors.forename: Esa K
authors.initials: EK
authors.lastname: Partio
authors.forename: Klaus
authors.initials: K
authors.lastname: Hedman
mesh_terms: D001706:Biopsy; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis*; D005838:Genotype; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D008969:Molecular Sequence Data; D016732:Parvovirus B19, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D012867:Skin / Q000821:virology*; D014162:Transfection
chemical_list: D004279:DNA, Viral; D008156:Luciferases