The development of an ELISA for chicken apolipoprotein II quantitation.
Polyclonal antibodies raised against chicken apoll was characterised for its use in Western blotting and ELISA detection systems of apoll in chicken plasma. The antibody has a high avidity and specificity for apolipoprotein II (apoII). Western blots show that the antibody reacts with a single band at 15 kDa. The antibody was used for setting up both direct and indirect ELISA assays for apoll. The indirect ELISA has a broader detection range (10-1,600U/mL) than the direct ELISA (10-100U/mL). It was found that both ELISA systems discriminate very well between vitellogenic (laying hen) and non-vitellogenic (rooster) plasma. The in- direct ELISA, due to its broad detection range, can potentially be used for monitoring female reproductive cycles, accidental and environmental exposure of males to estrogen, and for apoII secretion by cultured hepatocytes and hepatomas.
Pool
E J
EJ
Ecophysiology Laboratory, Zoology Department, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa.
van Wyk
J H
JH
Hermann
M
M
Ivessa
N E
NE
Hurter
E
E
D000818:Animals; D001053:Apolipoproteins / Q000032:analysis* / Q000737:chemistry*; D015153:Blotting, Western; D002645:Chickens; D004306:Dose-Response Relationship, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D008297:Male; D011498:Protein Precursors / Q000032:analysis* / Q000737:chemistry*
D001053:Apolipoproteins; D011498:Protein Precursors; C054949:apolipoprotein II