Involvement of active oxygen species in degradation of light-harvesting proteins under light stresses.
This paper presents evidence for light-mediated degradation of isolated light-harvesting proteins (Lhc2) and involvement of oxygen free radicals in the process. The time course of light harvesting photodestruction is much slower than that of D1 protein (requiring hours for complete breakdown). By use of mass spectrometry and amino acid sequencing, it has been revealed that the primary cleavages take place in the hydrophilic portion of the NH(2) region where oxygen-containing radicals attack randomly and not at specific sites. Moreover, these chlorophyll binding proteins are completely fragmented. From the effectiveness of scavengers and the preliminary electron paramagnetic resonance measurements reported, it appears that singlet oxygen is involved as a short-lived species, and hydroxyl and alkoxyl radicals act at higher light intensity or over a longer time, whereas hydrogen peroxide and superoxide anions are not observed. Antenna proteins appear more resistance to photodestruction in their monomeric form than in trimeric form, while minor antenna are highly sensitive. However, the organization of both minor and major proteins in the photosystem II supracomplex affords some photoprotection. Interestingly, leaves exposed to strong light contained degraded major antenna, unlike those kept in the dark, which is consistent with studies on the illumination of isolated proteins, supporting the hypothesis that active oxygen species play a role in vivo in the short-term acclimative adaptation of plants.
Zolla
Lello
L
Department of Environmental Science, University of Tuscia, Viterbo, Italy. zolla@unitus.it
Rinalducci
Sara
S
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D061146:Chlorophyll Binding Proteins; D008027:Light; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D010100:Oxygen / Q000737:chemistry; D018532:Pisum sativum; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000378:metabolism*; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D011487:Protein Conformation; D017382:Reactive Oxygen Species / Q000737:chemistry / Q000378:metabolism*; D018724:Spinacia oleracea; D020524:Thylakoids / Q000737:chemistry
D029681:Arabidopsis Proteins; C069660:CP29 light harvesting complex; D061146:Chlorophyll Binding Proteins; C490278:LHCA1 protein, Arabidopsis; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D010100:Oxygen