Simultaneous solid-phase extraction combined with liquid chromatography with ultraviolet absorbance detection for the determination of remifentanil and its metabolite in dog plasma.
To establish pharmacokinetic/pharmacodynamic relationships, a selective and specific high-performance liquid chromatographic method was developed for the quantitation of remifentanil and its metabolite in dog plasma. The assay involves a solid-phase extraction and a reversed-phase chromatographic separation with ultraviolet detection (lambda=210 nm). The calibration curves are linear in the range of 7.89-1500 ng ml(-1). Intra-day assay variability is less than 7% for all standards evaluated. Good recovery, linearity, accuracy, and precision were achieved with the assay that proved readily applicable to pharmacokinetic studies in dogs.
Kabbaj
M
M
Faculté de Pharmacie, Université de Montréal, Quebec H3C 3J7, Montréal, Canada.
Varin
F
F
D000701:Analgesics, Opioid / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D010880:Piperidines / Q000097:blood* / Q000493:pharmacokinetics; D000077208:Remifentanil; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D000701:Analgesics, Opioid; D010880:Piperidines; D000077208:Remifentanil