Cytotoxicity of dentine-bonding agents on human pulp cells in vitro.
OBJECTIVE
To investigate the cytotoxicity of five different dentine-bonding agents on human pulp cells in vitro.


METHODS
Set specimens from Clearfil SE Bond (CB), Heliobond (HB), Prime & Bond NT (PB), Single Bond (SB), and Syntac Single Component (SC) were eluted with culture medium for 2 and 5 days. Cytotoxicity was judged using tetrazolium bromide reduction assay on human primary pulp cells.


RESULTS
Elutes from five dentine-bonding agents were cytotoxic to primary human pulp cells (P < 0.05). CB was the least toxic sealer amongst the chemicals tested. The cytotoxic response decreased in an order of SB > PB > SC > HB > CB.


CONCLUSIONS
The influence of the cytotoxicity depended on the materials tested. Dentine-bonding agents have significant potential for pulpal toxicity.
Huang
F-M
FM
Department of Prosthodontics, Chung Shan Medical University Hospital, Chung Shan Medical University, Taichung, Taiwan.
Chang
Y-C
YC
D000179:Acrylates / Q000633:toxicity; D000180:Acrylic Resins / Q000633:toxicity; D000704:Analysis of Variance; D001672:Biocompatible Materials / Q000633:toxicity*; D017438:Bisphenol A-Glycidyl Methacrylate / Q000633:toxicity; D018929:Cell Culture Techniques; D004396:Coloring Agents; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D017220:Dentin-Bonding Agents / Q000633:toxicity*; D006801:Humans; D011109:Polymethacrylic Acids / Q000633:toxicity; D019279:Resin Cements / Q000633:toxicity; D013223:Statistics as Topic; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
D000179:Acrylates; D000180:Acrylic Resins; D001672:Biocompatible Materials; C438313:Clearfil SE Bond; D004396:Coloring Agents; D017220:Dentin-Bonding Agents; D011109:Polymethacrylic Acids; C425843:Prime and Bond NT; D019279:Resin Cements; C078484:Syntac dentine adhesive; D013778:Tetrazolium Salts; D013844:Thiazoles; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; C040415:heliobond; C022616:thiazolyl blue