[Expression of HIF-1 alpha and its relationship to apoptosis and proliferation in lung cancer].
OBJECTIVE
Hipoxia-inducible factor-1 is a transcriptive factor that regulates genes involved in metabolism, angiogenesis, proliferation, and apoptosis. This study was designed to investigate the expression of hypoxia inducible facter-1 alpha(HIF-1 alpha) and its relationship to bcl-2, Bax, PCNA in lung cancer.


METHODS
Immunohistochemical streptavidin/peroxidase(SP) was used to examine the expression of HIF-1a, bcl-2, Bax, and PCNA in 60 cases of lung cancer.


RESULTS
In 60 cases of lung cancer, positive rate for HIF-1a was 28.3% (17/60), specially the positive rate of small cell lung cancer(66.7%) was significantly higher than non-small cell lung cancer (21.6%). HIF-1a expression increased as clinical stage and metastasis increased(P < 0.01). The positive rate of bcl-2, Bax, and PCNA were 31.7% (19/60), 40.0% (24/60), 76.7% (46/60), respectively. Inverse relationship was found between the expression of HIF-1 alpha and bcl-2; while the correlation of HIF-1 alpha and Bax was positive(P < 0.01). The relationship between HIF-1 alpha and Bax was positive(P < 0.01). The relationship between HIF-1 alpha and PCNA was not observed(P > 0.05).


CONCLUSIONS
HIF-1 alpha is correlated with apopotosis, but has no relationship with proliferation.
Fan
Li-fang
LF
Department of Pathology, Medical College, Wuhan University, Wuhan 430071, P. R. China. lifang fan@etang.com
Diao
Lu-ming
LM
Chen
De-ji
DJ
Liu
Ming-qiu
MQ
Zhu
Li-qin
LQ
Li
Hong-gang
HG
Tang
Zhi-jiao
ZJ
Xia
Dong
D
Liu
Xuan
X
Chen
Hong-lei
HL
D000328:Adult; D000368:Aged; D017209:Apoptosis; D002455:Cell Division / Q000502:physiology; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008175:Lung Neoplasms / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D014157:Transcription Factors / Q000096:biosynthesis* / Q000502:physiology; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D018809:Proliferating Cell Nuclear Antigen; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D014157:Transcription Factors; D051028:bcl-2-Associated X Protein