title: The effects of desferrioxamin and vitamin E as supplements to antibiotics in the treatment of peritonitis in rats.
abstract: OBJECTIVE
  The aim of the study was to determine the effects of vitamin E and the iron chelating agent desferrioxamin (Dfx), supplemented by clindamycin and gentamycin therapy, on peritonitis caused by caecal ligation of a puncture wound in an experimental model.
  
  
  METHODS
  One hundred and twenty Spraque Dawley rats were divided into eight groups. Three groups were used as controls; intraperitoneal (i.p.), subcutaneous (s.c.) and i.p. and s.c., respectively. Group 4 was treated with Dfx, Group 5 with vitamin E and Group 6 with antibiotics. Group 7 was treated with vitamin E in combination with antibiotics, and Group 8 with a combination of antibiotics and Dfx. The rats were studied for 14 days following treatment, and survivors then humanely dispatched. Post-mortem examination was undertaken on all the rats studied.
  
  
  RESULTS
  In the control groups, mortality at 14 days was 66%. Rats treated with antibiotics alone (Group 5) had a mortality rate of 40%. Those treated with a combination of antibiotics and vitamin E (Group 7), however, had a mortality rate of only 14%, and those treated with antibiotics and Dfx had a mortality rate of only 7%.
  
  
  CONCLUSIONS
  This study suggests that treatment of peritonitis in rats with a combination of Dfx and antibiotics has a significant beneficial effect on survival, in comparison with treatment with antibiotics alone.
authors.affiliation: Department of Anesthesiology, Istanbul Memorial Hospital, Istanbul, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Soybir
authors.forename: G
authors.initials: G
authors.lastname: Soybir
authors.forename: H
authors.initials: H
authors.lastname: Lice
authors.forename: K
authors.initials: K
authors.lastname: Dolay
authors.forename: A
authors.initials: A
authors.lastname: Ozşeker
authors.forename: F
authors.initials: F
authors.lastname: Köksoy
mesh_terms: D000818:Animals; D002981:Clindamycin / Q000627:therapeutic use; D003676:Deferoxamine / Q000627:therapeutic use*; D004359:Drug Therapy, Combination / Q000627:therapeutic use*; D005839:Gentamicins / Q000627:therapeutic use; D008297:Male; D010538:Peritonitis / Q000188:drug therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014810:Vitamin E / Q000627:therapeutic use*
chemical_list: D005839:Gentamicins; D014810:Vitamin E; D002981:Clindamycin; D003676:Deferoxamine