Characterisation of reversed-phase liquid chromatographic columns by chromatographic tests. Evaluation of 36 test parameters: repeatability, reproducibility and correlation.
The European Pharmacopoeia (Ph. Eur.) or other official compendia give only a general description of the stationary phase in the description of a liquid chromatographic method. Therefore the selection of a column giving suitable selectivity presents difficulties. Earlier, a test procedure was proposed that allows measurement of a number of parameters which are reported to be representative for stationary phase characteristics. This paper describes how the test procedure was applied on 69 RP-LC C18 columns. Chromatographic parameters obtained as test results were evaluated, and their repeatability, reproducibility and correlation were examined.
Visky
Dóra
D
Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Katholieke Universiteit Leuven, Van Evenstraat 4, B-3000 Leaven, Belgium.
Heyden
Yvan Vander
YV
Iványi
Tímea
T
Baten
Peggy
P
De Beer
Jacques
J
Kovács
Zsuzsanna
Z
Noszál
Béla
B
Roets
Eugène
E
Massart
Désiré L
DL
Hoogmartens
Jos
J
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet