High-throughput assay of ( R)-phenylacetylcarbinol synthesized by pyruvate decarboxylase.
A novel assay has been developed for the detection of ( R)-phenylacetylcarbinol, ( R)-PAC, a chiral intermediate in the industrial synthesis of ephedrine. It is the product of a biotransformation of benzaldehyde catalysed by the enzyme pyruvate decarboxylase. The assay, using 2,3,5-triphenyltetrazolium chloride, enables high-throughput photometric analysis of the activity of the enzyme thus avoiding time-consuming chromatographic procedures.
Breuer
Michael
M
BASF AG, GVF/E-A030, 67056 Ludwigshafen, Germany. Michael.Breuer@basf-ag.de
Pohl
Martina
M
Hauer
Bernhard
B
Lingen
Bettina
B
D000096:Acetone / Q000031:analogs & derivatives* / Q000032:analysis* / Q000138:chemical synthesis; D010783:Photometry / Q000379:methods*; D011767:Pyruvate Decarboxylase / Q000737:chemistry*; D013237:Stereoisomerism
D000096:Acetone; C035293:1-hydroxy-1-phenyl-2-propanone; D011767:Pyruvate Decarboxylase