Atrazine mineralization potential in two wetlands.
The fate of atrazine in agricultural soils has been studied extensively but attenuation in wetland systems has received relatively little attention. The purpose of this study was to evaluate the mineralization of atrazine in two wetlands in central Ohio. One was a constructed wetland, which is fed by Olentangy River water from an agricultural catchment area. The other was a natural fen (Cedar Bog) in proximity to atrazine-treated cornfields. Atrazine mineralization potential was measured by 14CO2 evolution from [U-ring-14C]-atrazine in biometers. The constructed wetland showed 70-80% mineralization of atrazine within 1 month. Samples of wetland water that were pre-concentrated 200-fold by centrifugation also mineralized 60-80% of the added atrazine. A high extent of atrazine mineralization (75-81% mineralized) was also associated with concentrated water samples from the Olentangy River that were collected upstream and downstream of the wetland. The highest levels of mineralization were localized to the top 5 cm zone of the wetland sediment, and the activity close to the outflow at the Olentangy wetland was approximately equal to that near the inflow. PCR amplification of DNA extracted from the wetland sediment samples showed no positive signals for the atzA gene (atrazine chlorohydrolase), while Southern blots of the amplified DNA showed positive bands in five of the six Olentangy wetland sediment samples. Amplification with the trzD (cyanuric acid amidohydrolase) primers showed a positive PCR signal for all Olentangy wetland sediment samples. There was little mineralization of atrazine in any of the Cedar Bog samples. DNA extracted from Cedar Bog samples did not yield PCR products, and the corresponding Southern hybridization signals were absent. The data show that sediment microbial communities in the Olentangy wetland mineralize atrazine. The level of activity may be related to the seasonality of atrazine runoff entering the wetland. Comparable activity was not observed in the Cedar Bog, perhaps because it does not directly receive agricultural runoff. Qualitatively, the detection of the genes was associated with measurable mineralization activity which was consistent with the differences between the two study sites.
Anderson
Kristen L
KL
Department of Microbiology, Ohio State University, Columbus 43210-1292, USA.
Wheeler
Kevin A
KA
Robinson
Jayne B
JB
Tuovinen
Olli H
OH
D000383:Agriculture; D001280:Atrazine / Q000737:chemistry* / Q000378:metabolism; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000032:analysis; D017753:Ecosystem; D019015:Geologic Sediments / Q000382:microbiology; D006540:Herbicides / Q000737:chemistry* / Q000378:metabolism; D010944:Plants; D016133:Polymerase Chain Reaction; D014873:Water Pollutants / Q000032:analysis*
D004269:DNA, Bacterial; D006540:Herbicides; D014873:Water Pollutants; D001280:Atrazine