title: Development of DNA chip for the simultaneous detection of various beta-lactam antibiotic-resistant genes.
abstract: A robust and fast DNA chip method was developed in order to detect the various beta-lactam antibiotic-resistance genes in one slide. These genes included PSE, OXA, FOX, MEN, CMY, TEM, SHV, OXY, and AmpC. beta-lactam antibiotic-resistance genes were labeled with a fluorescent nucleotide by a multiplex polymerase chain reaction using a mixture of specific primer sets for each gene. This labeled target was hybridized with a DNA chip that contained the spots of the specific probe DNAs for each beta-lactam antibiotic-resistance gene. This technique made it possible to detect the specific resistance gene, even in a single bacterium.
authors.affiliation: Department of Biotechnology, Seoul Women's University, Korea.
authors.forename: Yeonhee
authors.initials: Y
authors.lastname: Lee
authors.forename: Choong-Sik
authors.initials: CS
authors.lastname: Lee
authors.forename: Yeo-Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Sungmin
authors.initials: S
authors.lastname: Chun
authors.forename: Seohyung
authors.initials: S
authors.lastname: Park
authors.forename: Young Sun
authors.initials: YS
authors.lastname: Kim
authors.forename: Byoung-Don
authors.initials: BD
authors.lastname: Han
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D018440:beta-Lactam Resistance / Q000235:genetics*