title: Molecular characterization of a cell wall-associated beta(1-3)endoglucanase of Aspergillus fumigatus.
abstract: A 74 kDa beta(1-3)endoglucanase of Aspergillus fumigatus was recently isolated from a cell wall autolysate and biochemically characterized. In this study, we report the cloning and the disruption of the ENGL1 gene encoding this beta(1-3)endoglucanase. ENGL1 contains an open reading frame of 2181 bp encoding a polypeptide of 727 amino acids. Sequence analysis showed that ENGL1 is the first characterized member of a new family of beta(1-3)glucanases. Disruption of ENGL1, however, did not lead to a phenotype distinct from the parental strain, indicating that this cell wall-associated beta(1-3)endoglucanase does not play an essential role in constitutive cell growth.
authors.affiliation: Institut Pasteur, Unité des Aspergillus, 25 rue du Docteur Roux 75724 Paris Cedex 15, France. imouyna@pasteur.fr
authors.forename: I
authors.initials: I
authors.lastname: Mouyna
authors.forename: J
authors.initials: J
authors.lastname: Sarfati
authors.forename: P
authors.initials: P
authors.lastname: Recco
authors.forename: T
authors.initials: T
authors.lastname: Fontaine
authors.forename: B
authors.initials: B
authors.lastname: Henrissatz
authors.forename: J P
authors.initials: JP
authors.lastname: Latge
mesh_terms: D000595:Amino Acid Sequence; D001232:Aspergillus fumigatus / Q000201:enzymology*; D002473:Cell Wall / Q000201:enzymology; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D010641:Phenotype
chemical_list: D004693:Glucan Endo-1,3-beta-D-Glucosidase