Human cytomegalovirus binding to DC-SIGN is required for dendritic cell infection and target cell trans-infection.
Cytomegalovirus (CMV) infection is characterized by host immunosuppression and multiorganic involvement. CMV-infected dendritic cells (DC) were recently shown to display reduced immune functions, but their role in virus dissemination is not clear. In this report, we demonstrated that CMV could be captured by DC through binding on DC-SIGN and subsequently transmitted to permissive cells. Moreover, blocking DC-SIGN by specific antibodies inhibited DC infection by primary CMV isolates and expression of DC-SIGN or its homolog DC-SIGNR rendered susceptible cells permissive to CMV infection. We demonstrated that CMV envelope glycoprotein B is a viral ligand for DC-SIGN and DC-SIGNR. These results provide new insights into the molecular interactions contributing to cell infection by CMV and extend DC-SIGN implication in virus propagation.
Halary
Franck
F
Laboratoire d'immunologie, CNRS UMR 5540, Université Bordeaux 2, France.
Amara
Ali
A
Lortat-Jacob
Hugues
H
Messerle
Martin
M
Delaunay
Thierry
T
Houlès
Corinne
C
Fieschi
Franck
F
Arenzana-Seisdedos
Fernando
F
Moreau
Jean François
JF
Déchanet-Merville
Julie
J
D015815:Cell Adhesion Molecules / Q000276:immunology* / Q000378:metabolism; D003587:Cytomegalovirus / Q000276:immunology* / Q000378:metabolism; D003586:Cytomegalovirus Infections / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D003713:Dendritic Cells / Q000276:immunology / Q000821:virology*; D006367:HeLa Cells; D006801:Humans; D037181:Lectins, C-Type / Q000276:immunology* / Q000378:metabolism; D008024:Ligands; D011956:Receptors, Cell Surface / Q000276:immunology* / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000276:immunology / Q000378:metabolism; D014779:Virus Replication / Q000276:immunology; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D008024:Ligands; D011956:Receptors, Cell Surface; D014759:Viral Envelope Proteins; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C040495:glycoprotein B, Simplexvirus