Use of a tyrosine hydroxylase mutant enzyme with reduced metal affinity allows detection of activity with cobalt in place of iron.
Ellis
Holly R
HR
Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843-2128, USA.
McCusker
Kevin P
KP
Fitzpatrick
Paul F
PF
D001665:Binding Sites; D001671:Biochemistry / Q000379:methods*; D003035:Cobalt / Q000032:analysis / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D009154:Mutation; D011622:Pterins / Q000378:metabolism; D013054:Spectrophotometry, Atomic; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics* / Q000378:metabolism*
D011622:Pterins; C011552:tetrahydropterin; D003035:Cobalt; D007501:Iron; D014446:Tyrosine 3-Monooxygenase