[Cell surface peroxidase--generator of superoxide anion in wheat root cells under wound stress].
Development of wound stress in excised wheat roots is known to be accompanied with an increase in reactive oxygen species (ROS) production, fall of membrane potential, release of K+ from cells, alkalization of extracellular solution, changes in respiration and metabolism of structural lipids. Dynamics of superoxide release correlates with changes in other physiological parameters, indicating the cross-reaction of these processes. Activity of peroxidase in extracellular solution after a 1 h incubation and removal of roots was shown to be stimulated by the range of organic acids, detergents, metals, and to be inhibited by cyanide. Superoxide production was sensitive to the addition of Mn2+ and H2O2. Increase in superoxide production correlates with the enhancement of peroxidase activity at the application of organic acids and detergents. The results obtained indicate that cell surface peroxidase is one of the main generators of superoxide in wounded wheat root cells. Different ways of stimulation of the ROS producing activity in root cells is supposed. By controlling superoxide and hydrogen peroxide formation, the cell surface peroxidase can control the adaptation processes in stressed plant cells.
Chasov
A V
AV
Institute of Biochemistry and Biophysics, Kazan' Research Center RAS. chasov@mail.knc.ru
Gordon
L Kh
LKh
Kolesnikov
O P
OP
Minibaeva
F V
FV
D002462:Cell Membrane / Q000201:enzymology; D018384:Oxidative Stress; D018517:Plant Roots / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism*; D014908:Triticum / Q000378:metabolism*
D013481:Superoxides