Effect of dietary sulfur amino acids on the taurine content of rat tissues.
The effect of dietary sulfur amino acids on the taurine content of rat blood and tissues was investigated. Three types of diet were prepared for this study: a low-taurine diet (LTD), normal taurine diet (NTD; LTD + 0.5% Met), and high-taurine diet (HTD; LTD + 0.5% Met + 3% taurine). These diets had no differing effect on the growth of the rats. The concentration of taurine in the blood from the HTD- and NTD-fed rats was respectively 1200% and 200% more than that from LTD-. In such rat tissues as the liver, the taurine content was significantly affected by dietary sulfur amino acids, resulting in a higher content with HTD and lower content with LTD. However, little or no effect on taurine content was apparent in the heart or eye. The activity for taurine uptake by the small intestine was not affected by dietary sulfur amino acids. The expression level of taurine transporter mRNA was altered only in the kidney under these dietary conditions: a higher expression level with LTD and lower expression level with HTD.
Satsu
H
H
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, Japan. asatsu@mail.ecc.u-tokyo.ac.jp
Kobayashi
Y
Y
Yokoyama
T
T
Terasawa
E
E
Shimizu
M
M
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D001835:Body Weight; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004032:Diet; D007421:Intestine, Small / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins; D008715:Methionine / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013455:Sulfur / Q000378:metabolism*; D013654:Taurine / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism*; D014020:Tissue Extracts / Q000737:chemistry; D000099247:Taurine Transporters
D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D008715:Methionine; D013455:Sulfur; D013654:Taurine; D014020:Tissue Extracts; D000099247:Taurine Transporters