[Effect of epidural morphine anesthesia on NO-ergic activity of nociceptive neurons of spinal ganglia and spinal cord].
The effect of epidural morphine anesthesia on NO-ergic mechanisms was studied during primary and secondary sensitization. Nitro-oxide synthase (NOS) activity was evaluated by the method of Home and Vincent (1989) (identification of NADPH diaphorase). NOS and NADPH diaphorase are two double enzymes in cells with synchronously changing activity. NOS activity corresponds to the level of NO secretion. The enzyme was measured on a microdensitometer. The findings evidence active involvement of NO-ergic mechanisms in the formation of primary and secondary hyperalgesia, and injection of morphine caused a significant decrease in the count of NO-expressing neurons in the total number of cells with high and average activities of the enzyme.
Shumatov
V B
VB
Shumatova
T A
TA
Balashova
T V
TV
D000328:Adult; D000767:Anesthesia, Epidural; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006930:Hyperalgesia / Q000378:metabolism; D008875:Middle Aged; D009020:Morphine / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009619:Nociceptors / Q000166:cytology / Q000378:metabolism; D012774:Shock, Traumatic / Q000209:etiology / Q000628:therapy; D013376:Substantia Gelatinosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014947:Wounds and Injuries / Q000150:complications
D009569:Nitric Oxide; D009020:Morphine; D019001:Nitric Oxide Synthase