Labeling of monoclonal antibodies with 153Sm for potential use in radioimmunotherapy.
The labeling of a monoclonal (anti-CEA) and a polyclonal (IgG) antibody with 153Sm has been investigated, using the bicyclic anhydride of DTPA (cDTPAa) as the chelating agent. The radiochemical study was performed using a combination of radioanalytical techniques (gel filtration, HPLC, ITLC-SG and SDS-PAGE). Optimization of factors affecting labeling (pH, Ab, Ab-DTPA concentration, etc.) leads to a labeling yield higher than 90%. Biodistribution studies in normal mice showed slow blood clearance and high uptake into the liver, kidney and lungs.
Fani
M
M
National Center of Scientific Research Demokritos, Athens, Greece.
Vranjes
S
S
Archimandritis
S C
SC
Potamianos
S
S
Xanthopoulos
S
S
Bouziotis
P
P
Varvarigou
A D
AD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification* / Q000493:pharmacokinetics / Q000627:therapeutic use; D002614:Chelating Agents; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D018796:Immunoconjugates / Q000302:isolation & purification* / Q000493:pharmacokinetics / Q000627:therapeutic use; D051379:Mice; D004369:Pentetic Acid / Q000031:analogs & derivatives*; D011854:Radiochemistry; D016499:Radioimmunotherapy; D011868:Radioisotopes / Q000302:isolation & purification* / Q000493:pharmacokinetics / Q000627:therapeutic use; D012493:Samarium / Q000302:isolation & purification* / Q000493:pharmacokinetics / Q000627:therapeutic use; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D018796:Immunoconjugates; D011868:Radioisotopes; C039777:cyclic DTPA anhydride; D012493:Samarium; D004369:Pentetic Acid