[Evaluation of Emit  netilmicin and amikacin assays on Dimension  RXL HM (Dade Behring) open channels].
The aim of this study was to evaluate and to validate an enzyme immunoassay in homogeneous phase for netilmicin and amikacin, adapted on the Dimension RXL HM (Dade Behring) machine. The results were compared with those obtained with automated polarization of fluorescence immunoassay using TDx FLx (Abbott). The protocol of the study and the analytical criteria were inspired by the protocol Valtec version 2002 recommended by the French Society of Clinical Biology (SFBC). The validation of this technique as adapted to the Dimension RXL HM has allowed its use for routine dosage adjustment of amikacin and netilmicin. The practicability is however the weak point of the adaptation of these techniques, even limiting as for their implementation.
Vételé
F
F
Laboratoire de biochimie, bâtiment 3B, Centre hospitalier Lyon-Sud, Chemin du grand Revoyet, 69495 Pierre-Bénite Cedex, France.
Manchon
M
M
Mialon
A
A
Berny
C
C
Baltassat
P
P
D000583:Amikacin / Q000097:blood*; D017248:Enzyme Multiplied Immunoassay Technique / Q000295:instrumentation; D006801:Humans; D009428:Netilmicin / Q000097:blood*; D012044:Regression Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D009428:Netilmicin; D000583:Amikacin