title: Peptide-beta2-microglobulin-MHC fusion molecules bind antigen-specific T cells and can be used for multivalent MHC-Ig complexes.
abstract: Recombinant soluble MHC molecules are widely used for visualization, activation and inhibition of antigen-specific immune responses. Using a genetic approach, we have generated two novel peptide-beta2-microglobulin-MHC constructs. We have linked the MHC molecule with the peptide of interest, without limiting the recognition by the cognate TCR. This molecule can also be joined with the IgG heavy chain resulting in a dimeric MHC-Ig fusion protein. These molecules bind antigen-specific T cells with high specificity and sensitivity, therefore, providing a valuable tool for detection as well as enrichment of antigen-specific T cells.
authors.affiliation: Abteilung Gastroenterologie, Hepatologie und Endokrinologie, Medizinische Hochschule Hannover, FZ-Oststadt, Raum 310, Pasteurallee 5, Germany. greten.tim@mh-hannover.de
authors.forename: Tim F
authors.initials: TF
authors.lastname: Greten
authors.forename: Firouzeh
authors.initials: F
authors.lastname: Korangy
authors.forename: Gunnar
authors.initials: G
authors.lastname: Neumann
authors.forename: Heiner
authors.initials: H
authors.lastname: Wedemeyer
authors.forename: Karola
authors.initials: K
authors.lastname: Schlote
authors.forename: Astrid
authors.initials: A
authors.lastname: Heller
authors.forename: Stephan
authors.initials: S
authors.lastname: Scheffer
authors.forename: Drew M
authors.initials: DM
authors.lastname: Pardoll
authors.forename: Annette I
authors.initials: AI
authors.lastname: Garbe
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Schneck
authors.forename: Michael P
authors.initials: MP
authors.lastname: Manns
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D019556:COS Cells; D002522:Chlorocebus aethiops; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D015789:HLA-A2 Antigen / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D001613:beta 2-Microglobulin / Q000235:genetics / Q000276:immunology*
chemical_list: D018984:Epitopes, T-Lymphocyte; D015789:HLA-A2 Antigen; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D001613:beta 2-Microglobulin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D004247:DNA