title: Whole-genome analysis of dorsal-ventral patterning in the Drosophila embryo.
abstract: The maternal Dorsal regulatory gradient initiates the differentiation of several tissues in the early Drosophila embryo. Whole-genome microarray assays identified as many as 40 new Dorsal target genes, which encode a broad spectrum of cell signaling proteins and transcription factors. Evidence is presented that a tissue-specific form of the NF-Y transcription complex is essential for the activation of gene expression in the mesoderm. Tissue-specific enhancers were identified for new Dorsal target genes, and bioinformatics methods identified conserved cis-regulatory elements for coordinately regulated genes that respond to similar thresholds of the Dorsal gradient. The new Dorsal target genes and enhancers represent one of the most extensive gene networks known for any developmental process.
authors.affiliation: Department of Molecular and Cell Biology, Division of Genetics and Development, 401 Barker Hall, University of California, Berkeley, Berkeley, CA 94720, USA.
authors.forename: Angelike
authors.initials: A
authors.lastname: Stathopoulos
authors.forename: Madeleine
authors.initials: M
authors.lastname: Van Drenth
authors.forename: Albert
authors.initials: A
authors.lastname: Erives
authors.forename: Michele
authors.initials: M
authors.lastname: Markstein
authors.forename: Michael
authors.initials: M
authors.lastname: Levine
mesh_terms: D000818:Animals; D019521:Body Patterning; D023081:CCAAT-Binding Factor / Q000235:genetics / Q000378:metabolism; D017124:Conserved Sequence; D004330:Drosophila / Q000196:embryology / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D047108:Embryonic Development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D016678:Genome; D008297:Male; D008648:Mesoderm; D008957:Models, Genetic; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D019076:Transgenes
chemical_list: D023081:CCAAT-Binding Factor; D029721:Drosophila Proteins