AcrAB Efflux System: Expression and Contribution to Fluoroquinolone Resistance in Klebsiella spp.
Seven Klebsiella pneumoniae and four Klebsiella oxytoca clinical isolates with different levels of resistance to ciprofloxacin were studied. Mutations in the topoisomerase genes were found in almost all strains, but the contribution of a multidrug efflux system homologous to AcrAB in Escherichia coli was also observed. Overexpression of this efflux system was demonstrated by immunoblotting with antibodies against E. coli AcrA.
Mazzariol
Annarita
A
Dipartimento di Patologia, Sezione di Microbiologia, Università di Verona, Italy. annarita.mazzariol@univr.it
Zuliani
Jessica
J
Cornaglia
Giuseppe
G
Rossolini
Gian Maria
GM
Fontana
Roberta
R
D000890:Anti-Infective Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000378:metabolism / Q000494:pharmacology; D004264:DNA Topoisomerases, Type I / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D041121:Klebsiella oxytoca / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D008826:Microbial Sensitivity Tests
D000890:Anti-Infective Agents; D002939:Ciprofloxacin; D004264:DNA Topoisomerases, Type I