Immunodominance of H60 is caused by an abnormally high precursor T cell pool directed against its unique minor histocompatibility antigen peptide.
The H60 minor histocompatibility (H) antigen peptide is derived from a glycoprotein that serves as a ligand for the stimulatory NKG2D receptor. We show that this peptide is remarkably immunodominant in that it competes effectively with MHC alloantigens, is efficiently crosspresented by host antigen-presenting cells (APCs), and readily elicits naive CD8 T cell responses in vitro. H60 immunodominance is neither a consequence of NKG2D engagement nor competition among minor H antigens on APCs. Instead, H60 immunodominance is a consequence of an abnormally high naive precursor frequency of H60 peptide reactive CD8 T cells. Understanding why the H60 peptide is so immunogenic has important implications in tissue transplantation and vaccine design.
Choi
Eun Young
EY
The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA.
Christianson
Gregory J
GJ
Yoshimura
Yoshitaka
Y
Sproule
Thomas J
TJ
Jung
Nadja
N
Joyce
Sebastian
S
Roopenian
Derry C
DC
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D016056:Immunodominant Epitopes; D008024:Ligands; D051379:Mice; D015778:Minor Histocompatibility Antigens / Q000276:immunology*; D008912:Minor Histocompatibility Loci / Q000276:immunology*; D055655:NK Cell Lectin-Like Receptor Subfamily K; D016377:Organ Transplantation; D011971:Receptors, Immunologic; D055607:Receptors, Natural Killer Cell; D014181:Transplantation Immunology
D016056:Immunodominant Epitopes; C527085:KLRK1 protein, human; C527086:Klrk1 protein, mouse; D008024:Ligands; D015778:Minor Histocompatibility Antigens; D055655:NK Cell Lectin-Like Receptor Subfamily K; D011971:Receptors, Immunologic; D055607:Receptors, Natural Killer Cell; C114735:minor H antigen H60