Mycobacterium avium subsp. paratuberculosis strains from cattle and sheep can be distinguished by a PCR test based on a novel DNA sequence difference.
A DNA sequence differing between sheep and cattle types of Mycobacterium avium subsp. paratuberculosis was identified and used to develop a PCR test. The test unequivocally distinguished all sheep types from cattle types and was negative for a wide range of other strains from the Mycobacterium avium-Mycobacterium intracellulare complex. The test will be useful for epidemiological purposes, particularly in hosts such as deer that can be easily infected with either type.
Collins
Desmond M
DM
AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand. desmond.collins@agresearch.co.nz
De Zoete
May
M
Cavaignac
Sonia M
SM
D000818:Animals; D015373:Bacterial Typing Techniques; D001483:Base Sequence / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis; D008969:Molecular Sequence Data; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010283:Paratuberculosis / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial