Vaccination of mice against invasive aspergillosis with recombinant Aspergillus proteins and CpG oligodeoxynucleotides as adjuvants.
In a murine model of invasive pulmonary aspergillosis, dendritic cells (DCs) pulsed with Aspergillus antigens induced the activation of CD4(+) Th1 cells capable of conferring resistance to the infection. Here we show that the combined, local delivery of unmethylated CpG oligodeoxynucleotides (ODNs) and the Asp f 16 Aspergillus allergen resulted in the functional maturation and activation of airway DCs capable of inducing Th1 priming and resistance to the fungus. Therefore, ODNs act as a potent adjuvant for the vaccine-induced protection against the fungus by promoting dominant Th1 response to Aspergillus antigens and allergens.
Bozza
Silvia
S
Microbiology section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy.
Gaziano
Roberta
R
Lipford
Grayson B
GB
Montagnoli
Claudia
C
Bacci
Angela
A
Di Francesco
Paolo
P
Kurup
Viswanath P
VP
Wagner
Hermann
H
Romani
Luigina
L
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001228:Aspergillosis / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D001232:Aspergillus fumigatus / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000235:genetics / Q000276:immunology; D005657:Fungal Vaccines; D007249:Inflammation / Q000276:immunology; D008172:Lung Diseases, Fungal / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000627:therapeutic use; D018417:Th1 Cells / Q000276:immunology
D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D005656:Fungal Proteins; D005657:Fungal Vaccines; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins