Influence of the storage time and the method of stimulation on whole blood chemiluminescence.
The ultra-weak light, chemiluminescence (CL), of stimulated leukocytes is a well-known phenomenon. Parameters of this CL are modified by many factors including laboratory procedures. The order of stimulation and enhancement (two possibilities) and two concentrations of luminol create four types of procedure, which were accomplished in five sample storage 'time points'. We received the strongest signals of CL using higher concentrations of luminol (and DMSO), but only when stimulation (FMLP) was used before enhancement (luminol); luminol used before FMLP strongly inhibited CL. For lower luminol concentration (and DMSO), the order of stimulation and enhancement was of no importance. There were comparable but weaker signals of CL in this case. We received stronger signals with storage time for all procedures. It may be dependent on the priming of phagocytes by releasing cell factors. Stimulation (FMLP) before enhancement (luminol) eliminates the inhibitory effect of DMSO on CL.
Podraza
Wojciech
W
Department of Medical Physics, Pomeranian Academy of Medicine, Szczecin, Poland. podrazaw@sci.pam.szczecin.pl
Gonet
Bolesław
B
Kordek
Agnieszka
A
D000328:Adult; D001769:Blood; D001793:Blood Preservation; D004121:Dimethyl Sulfoxide; D005260:Female; D005609:Free Radicals; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol; D008297:Male; D008875:Middle Aged; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010587:Phagocytosis; D012015:Reference Standards; D013997:Time Factors
D005609:Free Radicals; D009240:N-Formylmethionine Leucyl-Phenylalanine; D008165:Luminol; D004121:Dimethyl Sulfoxide