Creating polyketide diversity through genetic engineering.
Modular polyketide synthases (PKS) are large multifunctional enzymes that synthesize complex polyketides, a therapeutically important class of natural products. The linear order and composition of catalytic sites that comprise the PKS represent a "code" that determines the identity of the polyketide product. By re-programming the PKS through genetic engineering, it is possible to alter the code in a predictable manner to create specific structural modifications of polyketides and to produce new libraries of these natural products.
Kealey
James T
JT
Kosan Biosciences, 3832 Bay Center Place, Hayward, CA 94545, USA. kealey@kosan.com
D002240:Carbohydrate Sequence / Q000235:genetics; D034261:Epothilones / Q000737:chemistry / Q000235:genetics; D004917:Erythromycin / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D010455:Peptides / Q000737:chemistry / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics; D020123:Sirolimus / Q000737:chemistry / Q000378:metabolism
D034261:Epothilones; D009097:Multienzyme Complexes; D010455:Peptides; D012269:Ribosomal Proteins; D004917:Erythromycin; C114026:desoxyepothilone B; D020123:Sirolimus