[Characteristics of cyclodextrin production using cyclodextrin glucanotransferases of various groups of microorganisms].
Cyclodextrin glucanotransferases (CGTases; EC 2.4.1.19) from newly isolated mesophilic, thermophilic, alkalophilic, and halophilic bacilli, as well as from thermoactinomycetes, have been purified to homogeneity, and some of their physicochemical and biochemical characteristics (cyclizing, disproportionating, and hydrolytic activities) have been studied. Cyclodextrin (CD) production in the presence and absence of compounds favoring formation of complexes had certain specific features. We were able to demonstrate that CG-Tases of mesophilic and thermophilic strains form mixtures of alpha-, beta-, and gamma-CDs, whereas the enzymes from halophilic and alkalophilic microorganisms preferentially catalyze the formation of beta-CDs.
Abelian
V A
VA
Institute of Microbiology, National Academy of Sciences of Armenia, Abovyan, 378510 Armenia.
Balaian
A M
AM
Manukian
L S
LS
Afian
K B
KB
Meliksetian
V S
VS
Andreasian
N A
NA
Markocian
A A
AA
D001406:Bacillaceae / Q000201:enzymology*; D003505:Cyclodextrins / Q000032:analysis / Q000096:biosynthesis*; D005964:Glucosyltransferases / Q000737:chemistry / Q000378:metabolism*; D008848:Micromonosporaceae / Q000201:enzymology*; D008970:Molecular Weight; D013045:Species Specificity
D003505:Cyclodextrins; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase