Hypoxia activates multiple transcriptional pathways in mouse pheochromocytoma cells.
Mouse pheochromocytoma cells (MPCs) provide an excellent model system for investigating the effects of hypoxia on catecholamine enzyme genes and on transcription factors mediating stress responses. RT-PCR detects rapid, transient increases in PNMT mRNA in hypoxic MPC 712 cells. Additionally, elevation of mRNAs encoding transcription factors hypoxia inducible factor 1 (HIF-1) alpha subunit and Egr-1 are evident within 60 min incubation in anoxia. Therefore, hypoxia elicits rapid transcriptional responses in numerous genes expressed by chromaffin cells.
Evinger
Marian J
MJ
Department of Pediatrics, State University of New York at Stony Brook, Stony Brook, New York 11794, USA. mevinger@notes.cc.sunysb.edu
Cikos
Stefan
S
Nwafor-Anene
Victoria
V
Powers
James F
JF
Tischler
Arthur S
AS
D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000818:Animals; D002395:Catecholamines / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D000860:Hypoxia; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism; D010673:Pheochromocytoma / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014157:Transcription Factors; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D002395:Catecholamines; D004268:DNA-Binding Proteins; C497443:Hif1a protein, mouse; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors