title: New BAGE (B melanoma antigen) genes mapping to the juxtacentromeric regions of human chromosomes 13 and 21 have a cancer/testis expression profile.
abstract: A first BAGE (B melanoma antigen) gene, BAGE1, was identified because it encodes a human tumour antigen recognised by a cytolytic T lymphocyte. Here, we characterised five new BAGE genes mapping to the juxtacentromeric regions of human chromosomes 13 and 21 and nine BAGE gene fragments mapping to the juxtacentromeric regions of chromosomes 9, 13, 18, and 21. Genes and gene fragments share extensive regions of 90-99% nucleotide identity. We analysed the expression of BAGE genes on 215 tumours of various histological types and on nine normal tissues. Similar to BAGE1, the new BAGE genes are expressed in melanomas, bladder and lung carcinomas and in a few tumours of other histological types. All the normal tissues were negative, with the exception of testis. Our results show that human juxtacentromeric regions harbour genes, which are transcribed and translated, in addition to gene fragments that are generally not expressed. We suggest that the pattern of expression restricted to cancer/testis is a feature of the few genes mapping to juxtacentromeric regions.
authors.affiliation: Institut de Génétique Humaine, CNRS UPR 1142, 141, rue de la Cardonille, 34396 Montpellier, France.
authors.forename: Myriam
authors.initials: M
authors.lastname: Ruault
authors.forename: Pierre
authors.initials: P
authors.lastname: van der Bruggen
authors.forename: Marie-Elisabeth
authors.initials: ME
authors.lastname: Brun
authors.forename: Shelagh
authors.initials: S
authors.lastname: Boyle
authors.forename: Gérard
authors.initials: G
authors.lastname: Roizès
authors.forename: Albertina
authors.initials: A
authors.lastname: De Sario
mesh_terms: D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002503:Centromere / Q000235:genetics*; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics*; D009928:Organ Specificity; D020161:Physical Chromosome Mapping; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; C092706:BAGE protein, human; C490495:BAGE5 protein, human; D012333:RNA, Messenger