Serum deprivation-induced upregulation of voltage-dependent sodium channels in adrenal chromaffin cells: selective involvement of extracellular signal-regulated kinase pathway.
In the present study, we investigated whether activation of the MAPK family could regulate the cell surface expression of Na channels in cultured bovine adrenal chromaffin cells. The results suggest that constitutively activated ERK (but not p38 or JNK), by various extracellular stimuli, down-modulates the density of cell surface Na channels, which was mediated via the destabilization of Na channel alpha-subunit mRNA.
Yanagita
Toshihiko
T
Department of Pharmacology, Miyazaki Medical College, Miyazaki 889-1692, Japan. yanagita@post1.miyazaki-med.ac.jp
Kobayashi
Hideyuki
H
Yokoo
Hiroki
H
Saitoh
Tomokazu
T
Minami
Shin-Ichi
S
Shiraishi
Seiji
S
Uezono
Yasuhito
Y
Wada
Akihiko
A
D000818:Animals; D002417:Cattle; D019439:Chromaffin Cells / Q000378:metabolism*; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004789:Enzyme Activation; D015151:Immunoblotting; D048031:JNK Mitogen-Activated Protein Kinases; D020935:MAP Kinase Signaling System; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D015222:Sodium Channels / Q000378:metabolism*; D013997:Time Factors; D015854:Up-Regulation; D048051:p38 Mitogen-Activated Protein Kinases
D016895:Culture Media, Serum-Free; D012333:RNA, Messenger; D015222:Sodium Channels; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases