Intracytoplasmic immunoglobulin crystals in follicular lymphoma.
We report a case of follicular lymphoma with crystal inclusions. Swollen lymph nodes taken from the left neck of a 53- year-old Japanese woman were replaced by follicular proliferation of atypical centroblastic and centrocytic cells with intracytoplasmic crystal inclusions. The crystals were confined to lymphoma cells and were not found in histiocytes. Lymphoma cells were positively immunostained with lambda light chain and mu heavy chain, but the crystals were only weakly so. In situ hybridization of light chains disclosed a monoclonal expression of lambda light chain mRNA in lymphoma cells. The crystals had a periodic linear substructure with about 5-nm intervals. The worldwide literature reports 8 cases, including the current case of non-Hodgkin's lymphoma with crystals confined to the neoplastic cells. The cases did not accompany paraproteinemia and crystal-storing histiocytosis and appear to follow a favorable clinical outcome.
Wada
Ryuichi
R
Department of Pathology, Hirosaki University School of Medicine, Hirosaki, Japan.
Ebina
Yoshihito
Y
Kurotaki
Hidekachi
H
Yagihashi
Soroku
S
D003460:Crystallization; D003593:Cytoplasm / Q000737:chemistry / Q000473:pathology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007146:Immunoglobulin lambda-Chains / Q000032:analysis / Q000235:genetics / Q000648:ultrastructure; D007148:Immunoglobulin mu-Chains / Q000032:analysis / Q000235:genetics / Q000648:ultrastructure; D017403:In Situ Hybridization; D008198:Lymph Nodes / Q000737:chemistry / Q000473:pathology*; D008224:Lymphoma, Follicular / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis
D007146:Immunoglobulin lambda-Chains; D007148:Immunoglobulin mu-Chains; D012333:RNA, Messenger; D012334:RNA, Neoplasm