title: Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia.
abstract: Micro-RNAs (miR genes) are a large family of highly conserved noncoding genes thought to be involved in temporal and tissue-specific gene regulation. MiRs are transcribed as short hairpin precursors ( approximately 70 nt) and are processed into active 21- to 22-nt RNAs by Dicer, a ribonuclease that recognizes target mRNAs via base-pairing interactions. Here we show that miR15 and miR16 are located at chromosome 13q14, a region deleted in more than half of B cell chronic lymphocytic leukemias (B-CLL). Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority ( approximately 68%) of CLL cases.
authors.affiliation: Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA.
authors.forename: George Adrian
authors.initials: GA
authors.lastname: Calin
authors.forename: Calin Dan
authors.initials: CD
authors.lastname: Dumitru
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Shimizu
authors.forename: Roberta
authors.initials: R
authors.lastname: Bichi
authors.forename: Simona
authors.initials: S
authors.lastname: Zupo
authors.forename: Evan
authors.initials: E
authors.lastname: Noch
authors.forename: Hansjuerg
authors.initials: H
authors.lastname: Aldler
authors.forename: Sashi
authors.initials: S
authors.lastname: Rattan
authors.forename: Michael
authors.initials: M
authors.lastname: Keating
authors.forename: Kanti
authors.initials: K
authors.lastname: Rai
authors.forename: Laura
authors.initials: L
authors.lastname: Rassenti
authors.forename: Thomas
authors.initials: T
authors.lastname: Kipps
authors.forename: Massimo
authors.initials: M
authors.lastname: Negrini
authors.forename: Florencia
authors.initials: F
authors.lastname: Bullrich
authors.forename: Carlo M
authors.initials: CM
authors.lastname: Croce
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology; D015152:Blotting, Northern; D015153:Blotting, Western; D018956:CD5 Antigens / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D017353:Gene Deletion; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D006822:Hybrid Cells / Q000737:chemistry; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D009369:Neoplasms / Q000235:genetics / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000737:chemistry; D014066:Palatine Tonsil / Q000166:cytology; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000737:chemistry
chemical_list: D018956:CD5 Antigens; D035683:MicroRNAs; D012334:RNA, Neoplasm