title: Anti-nociceptive effects of diazepam binding inhibitor in the central nervous system of rats.
abstract: The present study investigated the effect of diazepam binding inhibitor (DBI) on nociception in the central nervous system of rats. There were dose-dependent increases in hindpaw withdrawal latency (HWL) to noxious thermal and mechanical stimulation after intrathecal injection of 1, 5 or 10 microg of DBI in rats, indicating a DBI-induced anti-nociceptive effect at the spinal levels of rats. Furthermore, it was found that there were no significant influences of intrathecal co-administration of gamma-aminobutyric acid (GABA) on the intrathecal DBI-induced increases in HWLs of rats. Intracerebroventricular administration of 1, 10 or 20 microg of DBI also induced dose-dependent increases in HWL to thermal and mechanical stimulation in rats, suggesting an anti-nociceptive effect of DBI in the brain. Moreover, there were no significant influences of intracerebroventricular co-administration of 2 microg of GABA on the intracerebroventricular DBI-induced increases in HWL of rats. The results of the present study demonstrated that DBI played anti-nociceptive effects in the central nervous system of rats.
authors.affiliation: Department of Physiology, College of Life Science, National Laboratory of Biomembrane and Membrane Biotechnology and Center for Brain and Cognitive Science, Peking University, Beijing 100871, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Dong-Chuan
authors.initials: DC
authors.lastname: Wu
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Long-Chuan
authors.initials: LC
authors.lastname: Yu
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000187:drug effects*; D026261:Diazepam Binding Inhibitor / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007276:Injections, Intraventricular; D007278:Injections, Spinal; D008297:Male; D010146:Pain / Q000188:drug therapy*; D010147:Pain Measurement; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time; D005680:gamma-Aminobutyric Acid / Q000008:administration & dosage / Q000502:physiology
chemical_list: D026261:Diazepam Binding Inhibitor; D005680:gamma-Aminobutyric Acid