[Study of the antiradical and radioprotective properties of S-containing compounds using the complex of in vitro test-systems].
Capability of S-radioprotectors (AET, 2-AT, 2-ADT) to react with OH-radicals and to protect various molecular biotest systems against radiation damage was compared with that of 5-methoxytryptamine, some amino acids and t-butanol. A method of competing acceptors was used to determine rate constants in reactions of the radioprotectors with OH-radicals. A complex of biotest systems (protein, DNA, protein-lipid complex) was applied to estimate the radioprotective activity in vitro. It was found that the studied S-compounds are capable of modifying the protective effect as compared to the expectation from the competitive kinetics approach. Both enhancing and lessening of the effect was observed depending on the test system used. The obtained results can be explained by the impact of secondary radicals on the bio-target and/or by the interaction of the S-compounds with the bio-target that altered its radiosensitivity.
Kondakova
N V
NV
Scientific Research Center of Biomedical Technology, Russian Academy of Agricultural Sciences, Moscow, 123056 Russia.
Sakharova
V V
VV
Ripa
N V
NV
Rebrov
L B
LB
Mandrugin
A A
AA
Fedoseev
V M
VM
D008735:5-Methoxytryptamine / Q000494:pharmacology; D000818:Animals; D004912:Erythrocytes / Q000187:drug effects / Q000528:radiation effects; D016166:Free Radical Scavengers; D005609:Free Radicals; D005720:Gamma Rays; D066298:In Vitro Techniques; D008297:Male; D008962:Models, Theoretical; D011829:Radiation Dosage; D011837:Radiation-Protective Agents / Q000494:pharmacology; D051381:Rats; D013844:Thiazoles / Q000494:pharmacology; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology
D016166:Free Radical Scavengers; D005609:Free Radicals; D011837:Radiation-Protective Agents; D013844:Thiazoles; D000338:beta-Aminoethyl Isothiourea; D008735:5-Methoxytryptamine; C031363:2-aminothiazoline