Isulinotropic properties of Nigella sativa oil in Streptozotocin plus Nicotinamide diabetic hamster.
The present study was designed to investigate the possible insulinotropic properties of Nigella sativa L. (N. sativa) oil in Streptozotocin plus Nicotinamide-induced diabetes mellitus in hamsters. Nicotinamide was injected intraperitoneally 15min before injection of Streptozotocin intravenously. Oral treatment with N. sativa oil began 4 weeks after induction of diabetes. Serum insulin was measured by enzymeimmunoassay. Islets insulin was stained using anti-insulin monoclonal antibody. Significant decrease in blood glucose level together with significant increase in serum insulin level were observed after treatment with N. sativa oil for 4 weeks. Big areas with positive immuno-reactivity for the presence of insulin were observed in the pancreases from N. sativa oil-treated group compared to non-treated one using immunohistochemical staining. Therefore, our data show that the hypoglycemic effect of N. sativa oil in Streptozotocin plus Nicotinamide diabetic hamsters resulted, at least partly, from a stimulatory effect on beta cell function with consequent increase in serum insulin level. These results indicate that N. sativa oil has insulinotropic properties in type 2-like model.
Fararh
K M
KM
Department of Physiology and Pathogenetic Veterinary Sciences, The United Graduate School, Gifu University, Japan.
Atoji
Y
Y
Shimizu
Y
Y
Takewaki
T
T
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D006224:Cricetinae; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy*; D007150:Immunohistochemistry; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D009536:Niacinamide / Q000008:administration & dosage; D010179:Pancreas / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology*; D013311:Streptozocin / Q000008:administration & dosage; D029974:Carum
D001786:Blood Glucose; D007328:Insulin; D010938:Plant Oils; D009536:Niacinamide; D013311:Streptozocin; C104525:caraway oil