Aldehyde-induced xanthine oxidase activity in raw milk.
In the present study, the aldehyde-induced pro-oxidative activity of xanthine oxidase was followed in an accelerated raw milk system using spin-trap electron spin resonance (ESR) spectroscopy. The aldehydes acetaldehyde, propanal, hexanal, trans-2-hexenal, trans-2-heptenal, trans-2-nonenal, and 3-methyl-2-butenal were all found to initiate radical reactions when added to milk. Formation of superoxide through aldehyde-induced xanthine oxidase activity is suggested as the initial reaction, as all tested aldehydes were shown to trigger superoxide formation in an ultrahigh temperature (UHT) milk model system with added xanthine oxidase. It was found that addition of aldehydes to milk initially increased the ascorbyl radical concentration with a subsequent decay due to ascorbate depletion, which renders the formation of superoxide in milk with added aldehyde. The present study shows for the first time potential acceleration of oxidative events in milk through aldehyde-induced xanthine oxidase activity.
Steffensen
Charlotte L
CL
Department of Animal Product Quality, Danish Institute of Agricultural Sciences, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark.
Andersen
Henrik J
HJ
Nielsen
Jacob H
JH
D000079:Acetaldehyde / Q000494:pharmacology; D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D008892:Milk / Q000201:enzymology*; D016877:Oxidants / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism*
D000447:Aldehydes; D016877:Oxidants; C013166:2-nonenal; C064600:2-heptenal; C051750:2-hexenal; C010463:n-hexanal; C005556:propionaldehyde; D014969:Xanthine Oxidase; D000079:Acetaldehyde