title: Acoustics of blood plasma on solid surfaces.
abstract: We have quantified surface associated coagulation of human blood plasma with a recently developed methodological system consisting of a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D), a method that measures the weight of adsorbed molecules on surfaces as a function of frequency shifts of a quartz crystal. Further, it measures the damping energy (i.e. viscoelasticity) of the adsorbed layer. Four different surfaces where studied: Heparin (Hep) surface as an active inhibitor of clot formation, titanium (Ti) surfaces that are known to activate the intrinsic pathway, polystyrene (PS) surfaces and poly(urethane urea) (PUUR) surfaces. The experiments were initiated by applying citrated human plasma at the sensor surfaces; calcium was then added toinitiate coagulation. The Hep surfaces showed no apparent indication of clot formation during one hour of incubation at room temperature. However, on Ti surfaces we observed an early and rapid change in both frequency shift and viscoelastic properties of the coagulating plasma. We inhibited the intrinsic pathway activation by using corn trypsin inhibitor (CTI), which is specific for factor FXIIa in the bulk phase, which prolonged the coagulation times for all non-heparinized surfaces. We have also found a peculiar initial plasma protein interaction phenomenon on Ti surfaces. The described methodology would be very efficient for basic studies of surface associated coagulation and as a screening method for new biomaterials.
authors.affiliation: Department of Cell and Molecular Biology/Interface biophysics, Lundberg Laboratory, Göteborg University, Box 462, SE-405 30 Göteborg, Sweden.
authors.forename: Marcus
authors.initials: M
authors.lastname: Andersson
authors.forename: Anders
authors.initials: A
authors.lastname: Sellborn
authors.forename: Camilla
authors.initials: C
authors.lastname: Fant
authors.forename: Christina
authors.initials: C
authors.lastname: Gretzer
authors.forename: Hans
authors.initials: H
authors.lastname: Elwing
mesh_terms: D000162:Acoustics; D000327:Adsorption / Q000187:drug effects; D001777:Blood Coagulation / Q000187:drug effects*; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing / Q000295:instrumentation / Q000379:methods*; D010949:Plasma / Q000737:chemistry*; D011137:Polystyrenes / Q000494:pharmacology; D011140:Polyurethanes / Q000494:pharmacology; D011791:Quartz; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology; D014894:Weights and Measures
chemical_list: D011137:Polystyrenes; D011140:Polyurethanes; D011791:Quartz; D006493:Heparin; C044690:polyetherurethane urea; D014025:Titanium