Adoptive immunotherapy for superficial bladder cancer with autologous macrophage activated killer cells.
OBJECTIVE
We assessed the efficacy and safety of adoptive immunotherapy administered to 17 patients with TaGIII or recurrent TaGII superficial bladder cancer following transurethral tumor resection.


METHODS
Macrophage activated killer (MAK) cells were obtained from autologous mononuclear cells harvested by apheresis, after in vitro culture for 7 days and activation with interferon-gamma on the last day of culture. The patients received 6 weekly intravesical infusions of approximately 2 x 10(8) cells each. Additionally, 5 patients received 2 or 3 more infusions at 3-month intervals. Each patient was followed for 1 year or until tumor recurrence, whichever came first.


RESULTS
A total of 112 intravesical infusions were performed. During the 12-month followup period 8 patients experienced 11 common toxicity criteria grade 1 or grade 2 adverse events considered possibly related to protocol. No clinically relevant grade 1 or 2 laboratory test results were reported while the patients received treatment. In 17 patients 8 tumors recurred compared to 34 recurrences during the year before the first MAK cell infusion. This difference was highly significant (p </=0.0005).


CONCLUSIONS
The promising efficacy and safety results of this study and the fact that the MAK cell treatment regimen proved feasible should encourage initiation of further large scale studies to confirm these data.
Thiounn
Nicolas
N
Service d'Urologie, Hôpital Cochin, France.
Pages
Franck
F
Mejean
Arnaud
A
Descotes
Jean-Luc
JL
Fridman
Wolf-H
WH
Romet-Lemonne
Jean-Loup
JL
D000283:Administration, Intravesical; D000368:Aged; D005260:Female; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000009:adverse effects; D007694:Killer Cells, Natural / Q000276:immunology*; D008262:Macrophage Activation; D008297:Male; D008875:Middle Aged; D001749:Urinary Bladder Neoplasms / Q000628:therapy*