Effects of various eye drops on corneal wound healing after superficial keratectomy in rabbits.
OBJECTIVE
The effects of various eye drops on corneal wound healing, particularly in the subepithelial haze area, were investigated histologically following superficial keratectomy in rabbits.


METHODS
Mechanical superficial keratectomy was performed in rabbit eyes. Tranilast, betamethasone, hyaluronic acid, and diclofenac eye drops were administered after the procedure. Physiological saline was used as a control. Corneas were excised 1, 2, 3, and 4 weeks after keratectomy, labeled with 3H-thymidine or 3H-proline, and subjected to autoradiography.


RESULTS
In the control and diclofenac groups, corneal haze occurred 3 weeks after keratectomy. Histological examination revealed an accumulation of proliferating keratocytes and active synthesis of collagen in the subepithelial area. In the tranilast and betamethasone groups, formation of corneal haze was reduced compared to the controls. The proliferation of keratocytes and the production of collagen in the corneal stroma were inhibited by these drugs. In the hyaluronic acid group also, corneal haze was decreased. In this group, although the proliferation of keratocytes was activated compared to the controls, abnormal accumulation of keratocytes in the subepithelial area was not detected.


CONCLUSIONS
Tranilast and betamethasone decrease the formation of subepithelial haze by inhibiting keratocyte proliferation and synthesis of extracellular matrix in the corneal stroma. Hyaluronic acid, on the other hand, inhibits subepithelial haze by promoting physiologic wound healing.
Tani
Emiko
E
Division of Organ Therapeutics, Department of Ophthalmology, Kobe University Graduate School of Medicine, Japan.
Katakami
Chikako
C
Negi
Akira
A
D000818:Animals; D000869:Anterior Eye Segment / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000627:therapeutic use; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage* / Q000627:therapeutic use; D001623:Betamethasone / Q000008:administration & dosage* / Q000494:pharmacology; D003315:Cornea / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D004008:Diclofenac / Q000008:administration & dosage* / Q000627:therapeutic use; D005260:Female; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000494:pharmacology; D009883:Ophthalmic Solutions; D013508:Ophthalmologic Surgical Procedures; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects*; D062367:ortho-Aminobenzoates / Q000008:administration & dosage* / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D009883:Ophthalmic Solutions; D062367:ortho-Aminobenzoates; D004008:Diclofenac; D006820:Hyaluronic Acid; D001623:Betamethasone; C012293:tranilast