Chicken HDAC2 down-regulates IgM light chain gene promoter activity.
In a chicken B cell line, DT40, the disruption of HDAC2 (chHDAC2) gene causes an alteration of several gene expressions including chicken IgM light chain (chIgM-L) gene by 2D-PAGE analysis. To investigate the transcriptional function of chHDAC2, we employed the chIgM-L promoter reporter plasmid. We found that chHDAC2 represses activated chIgM-L promoter activity. In transient expression experiments in NIH 3T3 cell, the specific histone deacetylase inhibitor tricostatin A (TSA) increased transactivation of chIgM-L promoter activity mediated by chicken Oct-1 and OBF-1 proteins. In transient coexpression of the three class I chicken histone deacetylases (chHDAC1-3) tested, only chHDAC2 repressed the activated chIgM-L promoter activity. These findings suggest that chHDAC2 might be recruited to the chIgM-L promoter and specifically repress chIgM-L transcription.
Takechi
Shinji
S
Department of Biochemistry, Miyazaki Medical College, Kihara, Kiyotake, 889-1692, Miyazaki, Japan. stakechi@post1.miyazaki-med.ac.jp
Adachi
Masaru
M
Nakayama
Tatsuo
T
D016475:3T3 Cells; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D002460:Cell Line; D002645:Chickens; D015536:Down-Regulation; D005803:Genes, Immunoglobulin; D056464:Histone Deacetylase 2; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases / Q000502:physiology*; D006877:Hydroxamic Acids / Q000494:pharmacology; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007075:Immunoglobulin M / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008957:Models, Genetic; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000037:antagonists & inhibitors / Q000502:physiology*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D012097:Repressor Proteins; C012589:trichostatin A; C533424:Hdac2 protein, mouse; D056464:Histone Deacetylase 2; D006655:Histone Deacetylases