Developmentally regulated expression of Neuro-p24 and its possible function in neurite extension.
Process extension is a most marked and characteristic neuronal feature that is observed during the development, regeneration and plasticity of nervous system tissues. Neuro-p24, a novel membranous protein with a molecular weight of 24 kDa, is specifically localized in neurons, particularly in the neurites. Based on its molecular structure and distribution pattern in the brain we proposed that Neuro-p24 plays a role in neurite extension. In the present study we have made several findings that support this hypothesis; first, Neuro-p24 was abundant in motor axonal fibers, neurites of dorsal root ganglia neurons and apical dendrites of cerebral cortex neurons when their extension or arborization was proceeding very actively. Secondly, when COS-7 epithelial cells were transfected with either wild-type or deletion-mutated Neuro-p24 cDNAs, ectopic expression of wild-type cDNA caused morphological alterations resulting in a neuron-like appearance. These observations firmly support our proposal and indicate that Neuro-p24 plays an important role in the nervous tissue.
Araki
Masasuke
M
Developmental Neurobiology Laboratory, Department of Biological Sciences, Nara Women's University, Nara 630-8506, Japan. masaaraki@cc.nara-wu.ac.jp
Nagata
Kizashi
K
Satoh
Yumiko
Y
Kadota
Yoichi
Y
Hisha
Hiroko
H
Adachi
Yasushi
Y
Taketani
Shigeru
S
D000818:Animals; D000831:Animals, Newborn; D019556:COS Cells; D002454:Cell Differentiation / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007150:Immunohistochemistry; D051379:Mice; D009154:Mutation / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000172:deficiency* / Q000235:genetics; D009420:Nervous System / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D009473:Neuronal Plasticity / Q000502:physiology; D017966:Pyramidal Cells / Q000166:cytology / Q000378:metabolism
D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; C481124:Neuro-p24 protein, mouse