Enhancement of protein expression in insect cells by a lobster tropomyosin cDNA leader sequence.
We describe a cis element that dramatically increases the expression levels of exogenous genes in baculovirus-infected insect cells. This 21 bp sequence element is derived from a 5' untranslated leader sequence of a lobster tropomyosin cDNA (L21). By using a transfer vector carrying L21, the expression levels of tropomyosin and luciferase were 20- and seven-fold higher with L21 than without L21, respectively. L21 has both the Kozak sequence and the A-rich sequence found in the polyhedrin leader sequence. We assume that both sequence elements are essential for the enhancement of protein expression in the baculovirus-based expression system.
Sano
Ken-Ichi
K
RIKEN Harima Institute at SPring-8, Laboratory for Structural Biochemistry, Mikazuki, Sayo, Hyogo 679-5148, Japan.
Maeda
Kayo
K
Oki
Manami
M
Maéda
Yuichiro
Y
D020121:5' Untranslated Regions; D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D018076:DNA, Complementary; D033364:Decapoda; D005822:Genetic Vectors; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D018411:Spodoptera / Q000235:genetics*; D014335:Tropomyosin / Q000096:biosynthesis / Q000235:genetics*
D020121:5' Untranslated Regions; D018076:DNA, Complementary; D011994:Recombinant Proteins; D014335:Tropomyosin; D008156:Luciferases