DNA binding studies of ruthenium(II) complexes containing asymmetric tridentate ligands.
Absorption spectroscopy, fluorescence spectroscopy and viscosity measurements have been used to characterize the DNA binding of [Ru(tpy)(dppt)](2+) (tpy=2,2':6',2"-terpyridine, dppt=3-(1,10-phenanthrolin-2-yl)-5,6-diphenyl-as-triazine), [Ru(tpy)(pta)](2+) (pta=3-(1,10-phenanthrolin-2-yl)-as-triazino[5,6-f]acenaphthylene) and [Ru(tpy)(ptp)](2+) (ptp=3-(1,10-phenanthrolin-2-yl)-as-triazino[5,6-f]-phenanthrene). The results indicate that [Ru(tpy)(pta)](2+) and [Ru(tpy)(ptp)](2+) bind with CT-DNA in an intercalative mode, while [Ru(tpy)(dppt)](2+) binds with DNA by partial intercalation. The ligand planarity of the complex has a significant effect on DNA binding affinity increases in the order [Ru(tpy)(dppt)](2+)<[Ru(tpy)(pta)](2+)<[Ru(tpy)(ptp)](2+).
Chao
Hui
H
Department of Chemistry, Zhongshan University, 510275, Guangzhou, PR China. ceschh@zsu.edu.cn
Mei
Wen Jie
WJ
Huang
Qi Wen
QW
Ji
Liang Nian
LN
D000818:Animals; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D005453:Fluorescence; D007364:Intercalating Agents / Q000737:chemistry; D008024:Ligands; D017975:Ruthenium Compounds / Q000737:chemistry*; D013057:Spectrum Analysis; D014783:Viscosity
D007364:Intercalating Agents; D008024:Ligands; D017975:Ruthenium Compounds; D004247:DNA; C427203:calf thymus DNA