title: Role of CAPS in dense-core vesicle exocytosis.
abstract: Calcium-dependent activator protein for secretion (CAPS) was initially identified in brain cytosol based on its ability to reconstitute calcium-triggered dense-core vesicle (DCV) exocytosis in permeable cell lines (PC12) of adrenal chromaffin origin. Current evidence indicates that CAPS functions selectively in DCV exocytosis by interacting with DCVs, the plasma membrane, and protein components of the fusion machinery. To further delineate the role of CAPS in endocrine and neural secretion, the tissue distribution of CAPS was determined. Immunoreactive CAPS I localized exclusively to neural and endocrine tissues including adrenal medulla, pancreatic islets, anterior pituitary, thyroid parafollicular C cells, gastrointestinal G cells, renal juxtaglomerular cells, and gray matter throughout the central nervous system. The results are consistent with a widespread functional role of CAPS in the regulated exocytosis of DCVs in the nervous and endocrine systems.
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
authors.forename: James J
authors.initials: JJ
authors.lastname: Wassenberg
authors.forename: Thomas F J
authors.initials: TF
authors.lastname: Martin
mesh_terms: D000313:Adrenal Medulla / Q000378:metabolism; D000818:Animals; D002135:Calcium-Binding Proteins; D002147:Calmodulin / Q000378:metabolism* / Q000502:physiology*; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D005089:Exocytosis; D005753:Gastric Mucosa / Q000378:metabolism; D007150:Immunohistochemistry; D007313:Insecta; D007515:Islets of Langerhans / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013961:Thyroid Gland / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: C485812:Cadps protein, rat; D002135:Calcium-Binding Proteins; D002147:Calmodulin; D012333:RNA, Messenger