title: Demonstration of Anaplasma (Ehrlichia) platys inclusions in peripheral blood platelets of a dog in Japan.
abstract: A free-roaming dog in Okinawa island showed Anaplasma (Ehrlichia) platys-like inclusions within the platelets of peripheral blood samples. The inclusions were positive in indirect fluorescence test with anti-A. phagocytophila serum. The platelet count of the dog was 170,000 microl(-1). The sequence analysis of the 16S rRNA, citrate synthase and heat shock protein genes of DNA from the infected platelets confirmed that the inclusions were A. platys. This is the first detection of A. platys inclusions in dogs in Japan.
authors.affiliation: Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan. inokuma@yamaguchi-u.ac.jp
authors.forename: Hisashi
authors.initials: H
authors.lastname: Inokuma
authors.forename: Kaori
authors.initials: K
authors.lastname: Fujii
authors.forename: Koutaro
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Masaru
authors.initials: M
authors.lastname: Okuda
authors.forename: Kenji
authors.initials: K
authors.lastname: Nakagome
authors.forename: Ryusei
authors.initials: R
authors.lastname: Kosugi
authors.forename: Munetaka
authors.initials: M
authors.lastname: Hirakawa
authors.forename: Takafumi
authors.initials: T
authors.lastname: Onishi
mesh_terms: D000709:Anaplasma / Q000235:genetics / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000097:blood / Q000469:parasitology*; D000818:Animals; D001792:Blood Platelets / Q000469:parasitology*; D004269:DNA, Bacterial / Q000235:genetics; D004283:Dog Diseases / Q000097:blood / Q000469:parasitology*; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D002479:Inclusion Bodies / Q000469:parasitology; D007564:Japan; D010976:Platelet Count / Q000662:veterinary; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S