title: Bacterial spores in silage and raw milk.
abstract: Spore-forming bacteria can survive food-processing treatments. In the dairy industry, Bacillus and Clostridium species determine the shelf-life of a variety of heat-treated milk products, mainly if the level of post-process contamination is low. In order to minimize problems caused by bacterial spores in foods and food production processes a chain management approach, from raw materials, ingredients and environmental sources to final product storage conditions, is most effective. Silage is considered to be a significant source of contamination of raw milk with spores. PCR-RAPD fingerprinting and heat resistance studies of populations of aerobic spore-formers isolated from grass and maize silage and from raw milk confirmed this assumption. Prevention of outgrowth of aerobic spores in silage will contribute to reduction of the total spore load of raw milk. Therefore, it is important that the silage fermentation process is controlled. Application of cultures of lactic acid bacteria or chemical additives can aid silage fermentation and improve aerobic stability.
authors.affiliation: NIZO Food Research, Department of Processing, Quality and Safety, Ede, The Netherlands. giffel@nizo.nl
authors.forename: M C
authors.initials: MC
authors.lastname: te Giffel
authors.forename: A
authors.initials: A
authors.lastname: Wagendorp
authors.forename: A
authors.initials: A
authors.lastname: Herrewegh
authors.forename: F
authors.initials: F
authors.lastname: Driehuis
mesh_terms: D000332:Aerobiosis; D000818:Animals; D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D004275:DNA, Ribosomal / Q000032:analysis; D006358:Hot Temperature; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D012820:Silage / Q000382:microbiology*; D013171:Spores, Bacterial / Q000254:growth & development*
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S