Neuroprotection by T-cells depends on their subtype and activation state.
This study analyzes how the antigen specificity, the subtype, and the activation state of T cells modulate their recently discovered neuroprotective potential. We assessed the prevention from neuronal damage in organotypic entorhinal-hippocampal slice cultures after co-culture with Th1 and Th2 cells either specific for myelin basic protein (MBP) or ovalbumin (OVA). We found that MBP-specific Th2 cells were the most effective in preventing central nervous system (CNS) tissue from secondary injury. This neuroprotective T cell effect appears to be mediated by soluble factors. After stimulation with phorbol myristate acetate and ionomycin, all T cells were most effective in preventing neuronal death. Our data show that the T cell subtype and activation state are important features in determining the neuroprotective potential of these cells.
Wolf
Susanne A
SA
Department of Cell and Neurobiology, Institute of Anatomy, Humboldt-University Hospital Charitè, 10098, Berlin, Germany.
Fisher
Jasmin
J
Bechmann
Ingo
I
Steiner
Barbara
B
Kwidzinski
Erik
E
Nitsch
Robert
R
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology / Q000276:immunology* / Q000503:physiopathology; D001930:Brain Injuries / Q000276:immunology* / Q000503:physiopathology; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000276:immunology*; D003260:Contact Inhibition / Q000276:immunology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D000939:Epitopes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D004676:Myelin Basic Protein / Q000276:immunology / Q000494:pharmacology; D009410:Nerve Degeneration / Q000276:immunology* / Q000503:physiopathology; D009474:Neurons / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018417:Th1 Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D018418:Th2 Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*
D016207:Cytokines; D000939:Epitopes; D004676:Myelin Basic Protein; D010047:Ovalbumin; D013755:Tetradecanoylphorbol Acetate