Molecular analysis of the accumulation of the transcripts of the large subunit gene of ribulose-1,5-bisphosphate carboxylase/oxygenase by light.
We previously reported that the rbcL transcript level was significantly increased by light signal [Lee and Sim (1995)]. To investigate whether or not the accumulation of rbcL mRNA by light signal is caused by an increase of transcription activity of the rbcL gene, runon transcription assays were performed The results indicated that the accumulation of rbcL mRNA is due to an increase in the transcriptional activity of the rbcL gene by light. An electrophoretic mobility shift assay (EMSA) was also carried out to elucidate the specific binding proteins that interact with the rbcL promoter region. As a result of EMSA, a plastid protein designated as the rbcL promoter binding (RLPB) protein factor was detected in the plastid extract of light-grown seedlings, but not in that of dark-adapted seedlings before harvesting. It was also ascertained in this study that the promoter core region for the RLPB protein factor to bind is between -3 and -32 nucleotide sequences from the transcription initiation site of the rbcL gene. These results suggest that the stimulation of rbcL transcription by light is, in part, due to the increase in binding of the RLPB protein factor to the rbcL promoter.
Kim
Jong Wan
JW
Division of Life Science, Korea University, Seoul.
Park
Jeong Koo
JK
Kim
Byung Hoon
BH
Lee
Jae-Seon
JS
Sim
Woong Seop
WS
D004268:DNA-Binding Proteins / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D008027:Light; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism*; D012273:Ribulose-Bisphosphate Carboxylase / Q000096:biosynthesis / Q000235:genetics* / Q000528:radiation effects; D014158:Transcription, Genetic / Q000528:radiation effects*; D003313:Zea mays / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D012273:Ribulose-Bisphosphate Carboxylase