Quinacrine enhances vesicular stomatitis virus inactivation and diminishes hemolysis of dimethylmethylene blue-phototreated red cells.
Several photodynamic methods for virus inactivation in red blood cell (RBC) suspensions have resulted in unwanted hemolysis during extended 1-6 degrees C storage. To explore the possibility that hemolysis may be mediated by a membrane-bound dye, a molecule similar in structure to yet different in light absorption properties from the photosensitizer was used as an inhibitor for RBC membrane binding in virus photoinactivation and photohemolysis studies. The addition of 500 pM quinacrine to oxygenated RBC before treatment with 3.6 microM dimethylmethylene blue (DMMB) and 219 mJ/cm2 red light resulted in an increased extracellular concentration of the sensitizer, increased extracelluar viral inactivation kinetics, and decreased hemolysis during 1-6 degrees C storage without alteration of quinacrine absorption properties. These results collectively suggest that despite its recognized affinity for viral nucleic acid, DMMB also binds to RBC membranes and that the bound dye is, in part, responsible for photoinduced hemolysis.
Wagner
Stephen J
SJ
American Red Cross Biomedical Services, Jerome H. Holland Laboratory for the Biomedical Sciences, Blood & Cell Therapy Development, Rockville, MD 20855, USA. wagners@usa.redcross.org
Skripchenko
Andrey
A
Thompson-Montgomery
Dedeene
D
D001793:Blood Preservation; D004347:Drug Interactions; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000528:radiation effects; D006461:Hemolysis / Q000187:drug effects* / Q000528:radiation effects; D008027:Light; D008751:Methylene Blue / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D017319:Photosensitizing Agents / Q000494:pharmacology; D011485:Protein Binding; D011796:Quinacrine / Q000494:pharmacology*; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects*; D038301:Virus Inactivation / Q000187:drug effects*
D017319:Photosensitizing Agents; C435946:dimethylmethylene blue; D011796:Quinacrine; D008751:Methylene Blue