title: Different activity of glyoxalase system enzymes in specimens of Sparus auratus exposed to sublethal copper concentrations.
abstract: The present study regards possible changes in the activity of glyoxalase system enzymes (glyoxalase I, GI, and glyoxalase II, GII) in tissues (brain, liver and white muscle) of the mediterranean bony fish Sparus auratus after a 20 days exposure to sublethal concentrations (0.1 or 0.5 ppm) of Cu in the marine water and on control untreated animals. The experiments also included measurements of copper concentration in the tissues, as well as of lactate dehydrogenase (LDH) activity, to evaluate possible Cu accumulation and changes in glycolytic activity respectively. Cu accumulation only occurs in the liver. GI, GII and LDH activities kept unchanged in the brain after copper exposure. GI activity in liver and muscle of copper-exposed animals decreases probably for a slackening in the glycolytic rate, as suggested by the lowering of LDH activity. GII activity remains unchanged or increases (liver extract, 0.5 ppm of Cu), maybe to safeguard enough cellular levels of GSH.
authors.affiliation: Department of Experimental Medicine, Division of Cellular and Molecular Biology, University of Perugia, Via del Giochetto, 06122 Perugia, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Antognelli
authors.forename: Rita
authors.initials: R
authors.lastname: Romani
authors.forename: Francesca
authors.initials: F
authors.lastname: Baldracchini
authors.forename: Alessandra
authors.initials: A
authors.lastname: De Santis
authors.forename: Giulia
authors.initials: G
authors.lastname: Andreani
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Talesa
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D003300:Copper / Q000493:pharmacokinetics / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007791:Lactoylglutathione Lyase / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D011765:Pyruvaldehyde / Q000378:metabolism; D021541:Sea Bream / Q000378:metabolism*; D013869:Thiolester Hydrolases / Q000378:metabolism*; D000099301:Hydroxyacylglutathione Hydrolase
chemical_list: D003300:Copper; D005978:Glutathione; D007770:L-Lactate Dehydrogenase; D007791:Lactoylglutathione Lyase; D011765:Pyruvaldehyde; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase