Influences of a dietary fatty acid composition on the emergence of glutathione S-transferase-P (GST-P) positive foci in the liver of carcinogen-treated rats.
The rats treated with a single i.p. injection of diethylnitrosoamine (DEN) and percial hepatectomy were fed for 11 weeks with a high fat diet mixed with 10% lard, eicosapentaenoic-acid-rich oil (EPA-oil) or arachidonic-acid-rich oil (AA-oil) and the emergence of glutathione S-transferase placental form (GST-P) in the liver was evaluated. There were no significant differences in the serum aminotransferase activities. The molar ratio of n-6 and n-3 fatty acid in the liver phospholipids was significantly low in the EPA-oil group compared with the other groups. In the EPA-oil group, the area percent and the unit area of GST-P positive foci were significantly smaller than the other groups. In the AA-oil group, no significant differences were recognized in the quantitative values for GST-P positive foci compared with the control and lard groups. In conclusion, a hepatic neoplasmic lesion induced by DEN was suppressed with EPA-rich fish oil, and arachidonic-acid-rich oil showed no effect of suppression or acceleration.
Sasagawa
T
T
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, Soja, Japan. sasagawa@fhw.oka-pu.ac.jp
Kosai
K
K
Ota
Y
Y
Mori
M
M
Okita
M
M
D000284:Administration, Oral; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D001798:Blood Proteins / Q000032:analysis; D002273:Carcinogens / Q000494:pharmacology*; D004041:Dietary Fats / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid; D005227:Fatty Acids / Q000737:chemistry* / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D005982:Glutathione Transferase / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010743:Phospholipids / Q000737:chemistry; D051381:Rats; D015430:Weight Gain / Q000187:drug effects
D001798:Blood Proteins; D002273:Carcinogens; D004041:Dietary Fats; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid; D005982:Glutathione Transferase; D000410:Alanine Transaminase; C029310:lard