title: Post-analysis follow-up and validation of microarray experiments.
abstract: Measurement of gene-expression profiles using microarray technology is becoming increasingly popular among the biomedical research community. Although there has been great progress in this field, investigators are still confronted with a difficult question after completing their experiments: how to validate the large data sets that are generated? This review summarizes current approaches to verifying global expression results, discusses the caveats that must be considered, and describes some methods that are being developed to address outstanding problems.
authors.affiliation: Pathogenetics Unit, Laboratory of Pathology and Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.
authors.forename: Rodrigo F
authors.initials: RF
authors.lastname: Chuaqui
authors.forename: Robert F
authors.initials: RF
authors.lastname: Bonner
authors.forename: Carolyn J M
authors.initials: CJ
authors.lastname: Best
authors.forename: John W
authors.initials: JW
authors.lastname: Gillespie
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Flaig
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Hewitt
authors.forename: John L
authors.initials: JL
authors.lastname: Phillips
authors.forename: David B
authors.initials: DB
authors.lastname: Krizman
authors.forename: Michael A
authors.initials: MA
authors.lastname: Tangrea
authors.forename: Mamoun
authors.initials: M
authors.lastname: Ahram
authors.forename: W Marston
authors.initials: WM
authors.lastname: Linehan
authors.forename: Vladimir
authors.initials: V
authors.lastname: Knezevic
authors.forename: Michael R
authors.initials: MR
authors.lastname: Emmert-Buck
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D005544:Forecasting; D020869:Gene Expression Profiling / Q000379:methods / Q000592:standards*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000592:standards*; D011506:Proteins / Q000235:genetics; D011786:Quality Control; D012333:RNA, Messenger / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger