Prediction of chromatographic retention and protein identification in liquid chromatography/mass spectrometry.
Liquid chromatography coupled on- or off-line with mass spectrometry is rapidly advancing as a tool in proteomics capable of dealing with the inherent complexity in biology and complementing conventional approaches based on two-dimensional gel electrophoresis. Proteins can be identified by proteolytic digestion and peptide mass fingerprinting or by searching databases using short-sequence tags generated by tandem mass spectrometry. This paper shows that information on the chromatographic behavior of peptides can assist protein identification by peptide mass fingerprinting in liquid chromatography/mass spectrometry. This additional information is significant and already available at no extra experimental cost.
Palmblad
Magnus
M
The Angstrom Laboratory, Division of Ion Physics, Box 534, and Institute of Chemistry, Department of Analytical Chemistry, Box 531, Uppsala University, SE-751 21 Uppsala, Sweden. palmblad1@llnl.gov
Ramström
Margareta
M
Markides
Karin E
KE
Håkansson
Per
P
Bergquist
Jonas
J
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D016208:Databases, Factual; D006801:Humans; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification
D000596:Amino Acids; D011506:Proteins