Evaluation of carbazole degradation by Pseudomonas rhodesiae strain KK1 isolated from soil contaminated with coal tar.
In this study, strain KK1 isolated from coal tar-contaminated soil was found to be able to mineralize carbazole as a sole source of carbon by radiorespirometric analysis. KK1 cells pregrown on phenanthrene were able to mineralize carbazole much more rapidly than cells pregrown on naphthalene, suggesting a possible close linkage between the pathways for carbazole and phenanthrene catabolism. Also, Rieske-type iron sulfur center sequence of dioxygenase from KK1 was analyzed to evaluate carbazole catabolism by KK1. A gene cloned out from KK1 using a universal dioxygenase primer set was found a dioxygenase for initial catabolism of carbazole based on deduced amino acid sequences. Northern hybridization using the putative carbazole dixoygenase gene fragment as a probe provided the information that catabolism of carbazole might be greatly activated in phenanthrene-grown cells. Analysis of PLFAs extracted from KK1 cells exposed to carbazole revealed that lipids 10:0 3OH, 17:0 cyclo, and 18:0 were representatives produced or significantly increased in response to carbazole. Strain KK1 was identified as Pseudomonas species with 94% confidence when BIOLOG system was applied, as Pseudomonas sp. with over 90% confidence by total cellular compositions of fatty acid, and as Pseudomonas rhodesiae with 99% confidence by 16S rRNA sequence. Accordingly, strain KK1 was identified as Pseudomonas rhodesiae based on combination of the data, and designated Pseudomonas rhodesiae KK1. The phylogenetic tree based on 16S rRNA suggested that strain KK1 was far away in the phylogenetic distance from the strains that can degrade carbazole.
Yoon
Byoung-Jun
BJ
Research Institute for Basic Sciences, Cheju National University, Jeju 690-756, Korea.
Lee
Dong-Heon
DH
Kang
Yoon-Suk
YS
Oh
Duck-Chul
DC
Kim
Seung-Il
SI
Oh
Kye-Heon
KH
Kahng
Hyung-Yeel
HY
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001673:Biodegradation, Environmental; D002227:Carbazoles / Q000378:metabolism*; D003033:Coal Tar; D049308:Dioxygenases; D005227:Fatty Acids / Q000378:metabolism; D008958:Models, Molecular; D010105:Oxygenases / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011549:Pseudomonas / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D016415:Sequence Alignment; D012988:Soil Microbiology; D012989:Soil Pollutants
D001426:Bacterial Proteins; D002227:Carbazoles; D005227:Fatty Acids; D012989:Soil Pollutants; C041514:carbazole; D003033:Coal Tar; D010105:Oxygenases; D049308:Dioxygenases; C108434:carbazole 1,9a-dioxygenase