Induction of differentiation rescues HL-60 cells from Rana catesbeiana ribonuclease-induced cell death.
Rana catesbeiana ribonuclease (RC-RNase) exerted strong anti-tumor activity and its cytotoxicity was shown to correlate with differentiation stages of three different hepatoma cell lines. In this study, we demonstrate different RC-RNase cytotoxicity in undifferentiated HL-60 cells and in those that had been induced to differentiate by retinoic acid or dimethylsulfoxide. RC-RNase showed cytotoxicity in undifferentiated HL-60 cells, but not in HL-60 cells undergoing terminal differentiation. Furthermore, the caspase-9/caspase-3 pathway was activated when RC-RNase induced death in undifferentiated HL-60 cells and induction of differentiation led to a reversal of the caspase activation pathway.
Wei
Chyou Wei
CW
Graduate Institute of Life Sciences, National Defense Medical Center, 114, Taipei, Taiwan.
Hu
Chih Chi Andrew
CC
Tang
Chih Hang Anthony
CH
Lee
Ming Chieh
MC
Wang
Jaang Jiun
JJ
D029845:Amphibian Proteins / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western; D053148:Caspase 3; D053453:Caspase 9; D020169:Caspases / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects*; D002454:Cell Differentiation; D004722:Endoribonucleases / Q000494:pharmacology*; D004789:Enzyme Activation; D018922:HL-60 Cells; D006801:Humans; D011892:Rana catesbeiana
D029845:Amphibian Proteins; D004722:Endoribonucleases; C094067:pyrimidine-guanine sequence-specific ribonuclease, Rana catesbeiana; C505407:CASP3 protein, human; C506433:CASP9 protein, human; D053148:Caspase 3; D053453:Caspase 9; D020169:Caspases