title: Sm60, a mannose-binding protein from Schistosoma mansoni with inflammatory property.
abstract: We demonstrate here that a mannose-binding protein from Schistosoma mansoni, termed Sm60, was recovered in the mannose-eluted fraction (Man(+)) upon affinity chromatography on immobilised mannose of the soluble antigen fraction from adult worm tegument and cercariae. Sm60 was detected in the Man(+) fraction as a prominent doublet with an apparent molecular mass of 60-66 kDa by SDS-PAGE and appeared as a single band with a pI of approximately 6.9 by isoelectrofocusing. Sm60 was also detected in preparations of schistosomula extract and soluble egg antigens using a mouse polyclonal anti-Sm60 serum on immunoblotting assay. This antiserum demonstrated that Sm60 was localised on the tegument of S. mansoni adult worm. In order to determine the role of Sm60 in host-parasite interactions, we showed that Sm60 induced in vitro migration of human neutrophil in a dose-dependent manner and in vitro mast cell degranulation. Sm60 triggered these activities through its carbohydrate-binding site, since these activities were selectively inhibited by 0.2 M D-mannose, but not by 0.2 M D-galactose. Furthermore, Sm60 induced in vivo neutrophil migration. In contrast, mast cell-depleted rats presented a significant reduction of the neutrophil migration induced by Sm60 as compared with non-depleted controls. These data suggest that in vivo neutrophil migration induced by Sm60 is modulated by mast cell-dependent mechanisms. Sm60 might play a key role in the host-parasite interaction, and its characterization opens perspective to examine the role of this molecule in the biology of S. mansoni.
authors.affiliation: Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, 14049-900 Ribeirão Preto, SP, Brazil. accastel@rpm.fmrp.usp.br
authors.forename: A A M
authors.initials: AA
authors.lastname: Coelho-Castelo
authors.forename: A
authors.initials: A
authors.lastname: Panunto-Castelo
authors.forename: A N
authors.initials: AN
authors.lastname: Moreno
authors.forename: M
authors.initials: M
authors.lastname: Dias-Baruffi
authors.forename: M C
authors.initials: MC
authors.lastname: Jamur
authors.forename: C
authors.initials: C
authors.lastname: Oliver
authors.forename: M-C
authors.initials: MC
authors.lastname: Roque-Barreira
authors.forename: V
authors.initials: V
authors.lastname: Rodrigues
mesh_terms: D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D002846:Chromatography, Affinity; D004305:Dose-Response Relationship, Drug; D019084:Fluorescent Antibody Technique, Indirect; D015801:Helminth Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D006790:Host-Parasite Interactions; D008297:Male; D037601:Mannose-Binding Lectin / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D008407:Mast Cells / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D017208:Rats, Wistar; D012550:Schistosoma mansoni / Q000737:chemistry*
chemical_list: D015801:Helminth Proteins; D037601:Mannose-Binding Lectin; C473830:Sm60 lectin, Schistosoma mansoni