Interaction of FANCD2 and NBS1 in the DNA damage response.
Fanconi anaemia (FA) and Nijmegen breakage syndrome (NBS) are autosomal recessive chromosome instability syndromes with distinct clinical phenotypes. Cells from individuals affected with FA are hypersensitive to mitomycin C (MMC), and cells from those with NBS are hypersensitive to ionizing radiation. Here we report that both NBS cell lines and individuals with NBS are hypersensitive to MMC, indicating that there may be functional linkage between FA and NBS. In wild-type cells, MMC activates the colocalization of the FA subtype D2 protein (FANCD2) and NBS1 protein in subnuclear foci. Ionizing radiation activates the ataxia telangiectasia kinase (ATM)-dependent and NBS1-dependent phosphorylation of FANCD2, resulting in an S-phase checkpoint. NBS1 and FANCD2 therefore cooperate in two distinct cellular functions, one involved in the DNA crosslink response and one involved in the S-phase checkpoint response.
Nakanishi
Koji
K
Department of Pediatric Oncology, Dana-Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA.
Taniguchi
Toshiyasu
T
Ranganathan
Velvizhi
V
New
Helen V
HV
Moreau
Lisa A
LA
Stotsky
Maria
M
Mathew
Christopher G
CG
Kastan
Michael B
MB
Weaver
David T
DT
D'Andrea
Alan D
AD
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D018797:Cell Cycle Proteins / Q000235:genetics*; D002461:Cell Line, Transformed; D019457:Chromosome Breakage; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D004260:DNA Repair; D004351:Drug Resistance / Q000235:genetics; D005199:Fanconi Anemia / Q000235:genetics; D052236:Fanconi Anemia Complementation Group D2 Protein; D006801:Humans; D016685:Mitomycin / Q000494:pharmacology; D009687:Nuclear Proteins / Q000235:genetics*; D016196:S Phase / Q000235:genetics; D014466:Ultraviolet Rays
D000903:Antibiotics, Antineoplastic; D018797:Cell Cycle Proteins; D003432:Cross-Linking Reagents; C499905:FANCD2 protein, human; D052236:Fanconi Anemia Complementation Group D2 Protein; C112156:NBN protein, human; D009687:Nuclear Proteins; D016685:Mitomycin