Simultaneous determination of various macrolides by liquid chromatography/mass spectrometry.
Macrolides are frequently used in veterinary medicine as therapeutic and preventive agents for various diseases. It is difficult to determine macrolides simultaneously with conventional methods due to their similar structures. A simultaneous analysis for erythromycin, roxithromycin, tiamulin and tylosin with LC/MS has been developed. Separation was performed on C18 reversed phase column. Mobile phase was gradiently flowed with 10 mM ammonium acetate and methanol. The mass spectrometer was run in the positive mode and selective ion monitoring mode. The molecular ions were [M+H]+ form at m/z 837.5 for erythromycin, at m/z 859.5 for roxithromycin, at m/z 494.2 for tiamulin and at m/z 916.7 for tylosin. Limits of detection were in the range from 0.001 to 0.01 microg/g lower than their MRLs.
Hwang
Youn-Hwan
YH
Division of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chungnam National University.
Lim
Jong-Hwan
JH
Park
Byung-Kwon
BK
Yun
Hyo-In
HI
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004224:Diterpenes / Q000032:analysis*; D004917:Erythromycin / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D015575:Roxithromycin / Q000032:analysis*; D012680:Sensitivity and Specificity; D015645:Tylosin / Q000032:analysis*; D014730:Veterinary Medicine
D000900:Anti-Bacterial Agents; D004224:Diterpenes; D015575:Roxithromycin; D004917:Erythromycin; C014224:tiamulin; D015645:Tylosin