title: Replication-defective genomic herpes simplex vectors: design and production.
abstract: Herpes simplex virus (HSV) may be engineered to produce flexible and efficient gene delivery vectors. Recent advances in vector design and production have built on increasing understanding of the basic biology of HSV to minimise vector toxicity and exploit viral features that give rise to lifelong latent infection in the nervous system. In addition, the emerging picture of viral cell entry has allowed early steps to be taken towards targeting viral entry to predetermined cellular subsets. Recent work has established sound principles for the straightforward production of large-scale pure preparations of vector stocks for clinical applications.
authors.affiliation: Department of Molecular Genetics and Biochemistry, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA.
authors.forename: Edward A
authors.initials: EA
authors.lastname: Burton
authors.forename: Qing
authors.initials: Q
authors.lastname: Bai
authors.forename: William F
authors.initials: WF
authors.lastname: Goins
authors.forename: Joseph C
authors.initials: JC
authors.lastname: Glorioso
mesh_terms: D004274:DNA, Recombinant / Q000235:genetics / Q000627:therapeutic use; D003673:Defective Viruses / Q000235:genetics / Q000302:isolation & purification; D015967:Gene Expression Regulation, Viral; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics* / Q000302:isolation & purification* / Q000627:therapeutic use; D016679:Genome, Viral; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D009369:Neoplasms / Q000628:therapy; D009422:Nervous System Diseases / Q000628:therapy*; D011995:Recombination, Genetic; D014779:Virus Replication / Q000235:genetics
chemical_list: D004274:DNA, Recombinant