Gene family of oleosin isoforms and their structural stabilization in sesame seed oil bodies.
Oleosins are structural proteins sheltering the oil bodies of plant seeds. Two isoform classes termed H- and L-oleosin are present in diverse angiosperms. Two H-oleosins and one L-oleosin were identified in sesame oil bodies from the protein sequences deduced from their corresponding cDNA clones. Sequence analysis showed that the main difference between the H- and L-isoforms is an insertion of 18 residues in the C-terminal domain of H-oleosins. H-oleosin, presumably derived from L-oleosin, was duplicated independently in several species. All known oleosins can be classified as one of these two isoforms. Single copy or a low copy number was detected by Southern hybridization for each of the three oleosin genes in the sesame genome. Northern hybridization showed that the three oleosin genes were transcribed in maturing seeds where oil bodies are being assembled. Artificial oil bodies were reconstituted with triacylglycerol, phospholipid, and sesame oleosin isoforms. The results indicated that reconstituted oil bodies could be stabilized by both isoforms, but L-oleosin gave slightly more structural stability than H-oleosin.
Tai
Sorgan S K
SS
Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University, Taichung, Taiwan 40227, ROC.
Chen
Miles C M
MC
Peng
Chi-Chung
CC
Tzen
Jason T C
JT
D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D015723:Gene Library; D007536:Isomerism; D008969:Molecular Sequence Data; D009391:Nephelometry and Turbidimetry; D010743:Phospholipids / Q000737:chemistry; D010940:Plant Proteins / Q000235:genetics*; D011061:Poly A / Q000096:biosynthesis; D012313:RNA / Q000302:isolation & purification; D012639:Seeds / Q000235:genetics; D012715:Sesame Oil / Q000235:genetics*; D031685:Sesamum / Q000235:genetics*; D014280:Triglycerides / Q000737:chemistry
D018076:DNA, Complementary; D010743:Phospholipids; D010940:Plant Proteins; D014280:Triglycerides; C110161:oleosin protein, Sesamum indicum; D011061:Poly A; D012313:RNA; D012715:Sesame Oil