title: Oxygen affinity of hemoglobin regulates O2 consumption, metabolism, and physical activity.
abstract: The oxygen affinity of hemoglobin is critical for gas exchange in the lung and O(2) delivery in peripheral tissues. In the present study, we generated model mice that carry low affinity hemoglobin with the Titusville mutation in the alpha-globin gene or Presbyterian mutation in the beta-globin gene. The mutant mice showed increased O(2) consumption and CO(2) production in tissue metabolism, suggesting enhanced O(2) delivery by mutant Hbs. The histology of muscle showed a phenotypical conversion from a fast glycolytic to fast oxidative type. Surprisingly, mutant mice spontaneously ran twice as far as controls despite mild anemia. The oxygen affinity of hemoglobin may control the basal level of erythropoiesis, tissue O(2) consumption, physical activity, and behavior in mice.
authors.affiliation: Department of Molecular Gerontology, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo 173-0015, Japan. sirasawa@tmig.or.jp
authors.forename: Takuji
authors.initials: T
authors.lastname: Shirasawa
authors.forename: Masahiko
authors.initials: M
authors.lastname: Izumizaki
authors.forename: Yo-ichi
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ishihara
authors.forename: Takahiko
authors.initials: T
authors.lastname: Shimizu
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Tamaki
authors.forename: Fei
authors.initials: F
authors.lastname: Huang
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Koizumi
authors.forename: Michiko
authors.initials: M
authors.lastname: Iwase
authors.forename: Hiromi
authors.initials: H
authors.lastname: Sakai
authors.forename: Eishun
authors.initials: E
authors.lastname: Tsuchida
authors.forename: Kenji
authors.initials: K
authors.lastname: Ueshima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Inoue
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Koseki
authors.forename: Toshiya
authors.initials: T
authors.lastname: Senda
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kuriyama
authors.forename: Ikuo
authors.initials: I
authors.lastname: Homma
mesh_terms: D000818:Animals; D006454:Hemoglobins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D010641:Phenotype; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011485:Protein Binding
chemical_list: D006454:Hemoglobins; D010100:Oxygen