title: RNase P RNA-mediated catalysis.
abstract: The endoribonuclease RNase P is involved in the processing of tRNA precursors to generate mature 5' termini. The catalytic activity of RNase P is associated with an RNA, RNase P RNA. A specific interaction between the 3' end of the substrate and RNase P RNA, to form an RNase P RNA-substrate complex, is referred to as the '73-294-interaction'. This interaction has an important role for efficient and correct cleavage to occur. Here our understanding of the contribution of the 73-294-interaction and metal ions, with respect to efficient and correct cleavage in RNase P RNA-mediated catalysis, will be discussed.
authors.affiliation: Department of Cell and Molecular Biology, Box 596, Biomedical Center, SE-751 24 Uppsala, Sweden. Leif.Kirsebom@icm.uu.se
authors.forename: L A
authors.initials: LA
authors.lastname: Kirsebom
mesh_terms: D001483:Base Sequence; D002384:Catalysis; D004722:Endoribonucleases / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012313:RNA / Q000502:physiology*; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing; D016337:RNA, Catalytic / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D043262:Ribonuclease P
chemical_list: D012322:RNA Precursors; D016337:RNA, Catalytic; D012313:RNA; D012343:RNA, Transfer; D004722:Endoribonucleases; D043262:Ribonuclease P