A unique amino acid deletion in the chimpanzee Cyclin T1 does not affect Tat trans-activation of HIV.
We have cloned and sequenced the chimpanzee (Pan troglodytes) cyclin T1 cDNA and performed functional HIV-1 Tat trans-activation studies. A unique codon deletion leading to a deleted asparagine residue in the N-terminal region of the first cyclin domain was discovered. This mutation does not significantly change the trans-activation of HIV-1, suggesting that Tat-Cyclin T1 mediated transcription is not a major barrier to HIV replication in the chimpanzee.
Rollman
Erik N
EN
Swedish Institute for Infectious Disease Control and Microbiology and Tumorbiology Center, Stockholm, Sweden.
Lund
Lars H
LH
Sjöstrand
Dan E
DE
Leitner
Thomas
T
Wahren
Britta E
BE
D000818:Animals; D001483:Base Sequence; D016213:Cyclins / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D015696:Gene Products, tat; D015497:HIV-1 / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D002679:Pan troglodytes / Q000821:virology*; D015533:Transcriptional Activation; D054322:tat Gene Products, Human Immunodeficiency Virus
D016213:Cyclins; D015696:Gene Products, tat; D054322:tat Gene Products, Human Immunodeficiency Virus