title: Quality assessment of Mycobacterium tuberculosis genotyping in a large laboratory network.
abstract: Quality assessment exercises were conducted to evaluate the reproducibility of IS6110 DNA fingerprinting performed by eight laboratories in the National Tuberculosis Genotyping and Surveillance Network. Three panels, each with 8 to 16 isolates, were typed at all laboratories, resulting in 280 images. When the pattern obtained by the majority for each isolate was used as the standard, exact matches were obtained for 73% of patterns; 90% and 97% of patterns matched within one- and two-band differences, respectively. A second approach involved retyping of randomly selected isolates at the Centers for Disease Control and Prevention. Retyping was done for 8-19 isolates per laboratory (76 total). Paired images matched exactly for 54% of isolates and within one and two band differences, 78% and 93%, respectively. We evaluated reasons for mismatching. We also evaluated the reproducibility of spoligotyping using a test panel of 13 isolates; a discrepancy of 1 in 91 results was noted.
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. cbraeden@cdc.gov
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Braden
authors.forename: Jack T
authors.initials: JT
authors.lastname: Crawford
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Schable
mesh_terms: D015373:Bacterial Typing Techniques / Q000592:standards*; D002487:Centers for Disease Control and Prevention, U.S. / Q000592:standards; D016172:DNA Fingerprinting / Q000592:standards; D004269:DNA, Bacterial / Q000032:analysis; D005838:Genotype; D006801:Humans; D007753:Laboratories / Q000458:organization & administration / Q000592:standards*; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D014376:Tuberculosis / Q000175:diagnosis / Q000382:microbiology / Q000517:prevention & control; D014481:United States
chemical_list: D004269:DNA, Bacterial