HLA-E-dependent presentation of Mtb-derived antigen to human CD8+ T cells.
Previous studies in mice and humans have suggested an important role for CD8+ T cells in host defense to Mtb. Recently, we have described human, Mtb-specific CD8+ cells that are neither HLA-A, B, or C nor group 1 CD1 restricted, and have found that these cells comprise the dominant CD8+ T cell response in latently infected individuals. In this report, three independent methods are used to demonstrate the ability of these cells to recognize Mtb-derived antigen in the context of the monomorphic HLA-E molecule. This is the first demonstration of the ability of HLA-E to present pathogen-derived antigen. Further definition of the HLA-E specific response may aid development of an effective vaccine against tuberculosis.
Heinzel
Amy S
AS
Division of Pulmonary & Critical Care Medicine, Portland VA Medical Center, Oregon Health Sciences University, Portland, OR 97201, USA.
Grotzke
Jeff E
JE
Lines
Rebecca A
RA
Lewinsohn
Deborah A
DA
McNabb
Andria L
AL
Streblow
Daniel N
DN
Braud
Veronique M
VM
Grieser
Heather J
HJ
Belisle
John T
JT
Lewinsohn
David M
DM
D017951:Antigen Presentation; D000942:Antigens, Bacterial / Q000276:immunology*; D015703:Antigens, CD / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D003713:Dendritic Cells / Q000502:physiology; D006680:HLA Antigens / Q000502:physiology*; D015395:Histocompatibility Antigens Class I / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D037181:Lectins, C-Type / Q000502:physiology; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D051920:NK Cell Lectin-Like Receptor Subfamily D; D011971:Receptors, Immunologic / Q000502:physiology; D055607:Receptors, Natural Killer Cell; D000097625:HLA-E Antigens
D000942:Antigens, Bacterial; D015703:Antigens, CD; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D007371:Interferon-gamma; D037181:Lectins, C-Type; D051920:NK Cell Lectin-Like Receptor Subfamily D; D011971:Receptors, Immunologic; D055607:Receptors, Natural Killer Cell; D000097625:HLA-E Antigens