Determination of ebrotidine and its metabolites by micellar electrokinetic capillary chromatography.
Ebrotidine and its potential metabolites were determined by micellar electrokinetic capillary chromatogaphy (MECC) using sodium dodecylsulfate (SDS) as surfactant. The influences of buffer composition, SDS concentration and addition of a neutral surfactant such as Brij 35 were studied. A 40 mM phosphate buffer at pH 7.50 containing 50 mM of SDS was selected as carrier electrolyte, and provided the optimum separation with regard to resolution and migration time. Linear calibration curves over the range studied (5.0-50 microg ml(-1)), limits of detection between 0.25 and 2.0 microg ml(-1) and run-to-run precision lower than 10% were obtained. The MECC method was applied to the determinaton of these compounds in spiked human urine.
Sentellas
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Department of Analytical Chemistry, University of Barcelona, Martí i Franquès 1-11, E-08028 Barcelona, Spain.
Puignou
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Galceran
M T
MT
D001557:Benzenesulfonates / Q000378:metabolism* / Q000652:urine; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006635:Histamine H2 Antagonists / Q000378:metabolism* / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012015:Reference Standards; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate; D013844:Thiazoles / Q000378:metabolism* / Q000652:urine
D001557:Benzenesulfonates; D002021:Buffers; D006635:Histamine H2 Antagonists; D013844:Thiazoles; D012967:Sodium Dodecyl Sulfate; C075156:ebrotidine