PCR-SSOP molecular typing of HLA-C alleles in an Iranian population.
HLA-C alleles were characterized by a polymerase chain reaction-sequence specific oligonucleotide probe (PCR-SSOP) hybridization protocol in a sample of 120 Iranians from Tehran. A total of 23 alleles were identified with the four most predominant--Cw*0401, Cw*0602, Cw*1202, and Cw*0701/06--accounting for almost 50% of HLA-C alleles. A comparison of HLA-C diversity among several populations indicates that Iranians stand at an intermediate genetic position between Europeans and Africans, an observation that may be related to their geographical location at a continental crossroads. The results also reveal a very high correlation between genetic and geographic distances on a global scale. A total of 30 HLA-C-DRB1 haplotypes were found in the Iranians, with the highest frequencies of 6.6% and 6.04 % being for Cw*0602-DRB1*0701 and Cw*1202-DRB1*1502, respectively.
Buhler
S
S
Laboratory of Genetics and Biometry, University of Geneva, Switzerland. stephane.buhler@anthro.unige.ch
Sanchez-Mazas
A
A
Zanone
R
R
Djavad
N
N
Tiercy
J-M
JM
D015343:DNA Probes, HLA / Q000235:genetics*; D005828:Genetics, Population; D015236:HLA-C Antigens / Q000235:genetics*; D006801:Humans; D007492:Iran; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic
D015343:DNA Probes, HLA; D015236:HLA-C Antigens