Protective effect of ginkgo biloba extract on endothelial cell against damage induced by oxidative stress.
The viability of bovine aortic endothelial cells (BAECs) treated with 0.1 m H O was decreased by 39.8%, and 100 mg/l EGb761 increased the viability by 20.6%. Exposure BAECs to H O for 6 min resulted in a significant elevation in the intracellular free Ca. Pretreatment of BAECs with 10 mg/l and 100 mg/l EGb761 for 10 min showed a decrease in the intracellular free Ca, 4.5% and 20.6%, respectively. The apoptotic rate of BAECs measured by propidium iodide (PI) staining was (38.1 +/- 2%) after 18 h of treatment with H O. Pretreatment of BAECs with 100 mg/l EGb761 for 1 h reduced the apoptotic rate to 27 +/- 1%. In addition, there were about 5-7% of cells stained positive measured by TUNEL assay. When BAECs were exposed to 0.1 m H O for 18 h, the number of TUNEL-positive cells increased to 37-44%. When 10 mg/l EGb761 and 100 mg/l EGb761 were used, the TUNEL-positive cells decreased to 26.5 +/- 3.1% and 17.5 +/- 1.7%, respectively. Furthermore, EGb761 also inhibited caspase-3 activity induced by H O. It is concluded that EGb761 has protective effect on bovine vascular endothelial cells against damage induced by H O. Further studies are needed to clarify the mechanisms of action of EGb761.
Ren
De Cheng
DC
Instiute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Du
Guan-Hua
GH
Zhang
Jun Tian
JT
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D020441:Ginkgo biloba; D006861:Hydrogen Peroxide / Q000009:adverse effects* / Q000037:antagonists & inhibitors; D020287:In Situ Nick-End Labeling / Q000379:methods; D018384:Oxidative Stress; D010936:Plant Extracts / Q000627:therapeutic use*; D000096983:Ginkgo Extract
D002118:Calcium; D053148:Caspase 3; D020169:Caspases; D006861:Hydrogen Peroxide; D010936:Plant Extracts; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract