[Yeast comparative genetics. A new MEL15 alpha-galactosidase gene of Saccharomyces cerevisiae].
To supplement the earlier identified European family of the highly homologous alpha-galactosidase MEL1-MEL11 genes and the African family of the divergent MEL12-MEL14 genes, a new MEL gene (MEL15) was found in several Saccharomyces cerevisiae strains isolated from maize dough in Ghana. Southern blotting and restriction enzyme analysis assigned the MEL15 gene to the African family and mapped it to chromosomes IV/XII, which migrate together in electrophoresis. Tetrad analysis ruled out the MEL15 location in the left arm of chromosome IV or the right arm of chromosome XII, which respectively contain the known MEL5 and MEL10 genes.
Naumov
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GI
Institute of Genetics and Selection of Industrial Microorganisms, Russian Academy of Sciences, Moscow, 113545 Russia. gnaumov@yahoo.com
Naumova
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Korshunova
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IV
Jakobsen
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D000349:Africa; D015139:Blotting, Southern; D002678:Chimera; D002874:Chromosome Mapping; D009154:Mutation; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D000519:alpha-Galactosidase / Q000235:genetics*
D029701:Saccharomyces cerevisiae Proteins; D000519:alpha-Galactosidase