title: Large deletions in mitochondrial DNA in radiation-associated human thyroid tumors.
abstract: Paired DNA samples of tumor and normal thyroid tissue from adult patients possibly exposed to radioactive Chernobyl fallout [11 cases of papillary thyroid carcinoma (PTC) and 6 follicular adenomas] and from control samples (9 PTC occurring in Japanese patients) were examined for the relative mitochondrial DNA (mtDNA) content, prevalence and level of common deletion (CD), and large-scale deletions in mtDNA. Elevated relative mtDNA content as estimated by real-time PCR was found in tumor tissue in most cases, but no significant correlation with the level of radioiodine contamination of patients' residency nor with clinicopathological data were found. CD was detected in every DNA specimen from all types of tissue regardless of the presence of oxyphillic cell changes. Elevated level of the CD was predominantly found in tumor tissue of the radiation-associated group but not in sporadic PTC. No correlation was noted with clinicopathological parameters, radioiodine contamination, and relative mtDNA content. The quantity of large-scale deletions in mtDNA was elevated in most tumor tissues, especially in the radiation-associated group and tended to correlate with the level of radiopollutant in PTC. In contrast to sporadic PTC, highly significant-positive correlation between the presence of large scale mtDNA deletions and relative mtDNA content was found in radiation-associated tumors (P = 0.001 and P = 0.019 in PTC and follicular adenoma, respectively). Normal tissue displayed the inverse tendency. No association with level of the CD was found in either group of cases. Concordant increase of both relative mtDNA content and number of mtDNA deletions was detected more often in radiation-associated PTC than in sporadic PTC. Thus, simultaneous determination of the number of large-scale mtDNA deletions and relative mtDNA content may be useful to elucidate molecular distinctive features of radiation-associated thyroid tumors.
authors.affiliation: Department of Molecular Medicine, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
authors.forename: Tatiana I
authors.initials: TI
authors.lastname: Rogounovitch
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Saenko
authors.forename: Yuki
authors.initials: Y
authors.lastname: Shimizu-Yoshida
authors.forename: Aleksandr Yu
authors.initials: AY
authors.lastname: Abrosimov
authors.forename: Eugeny F
authors.initials: EF
authors.lastname: Lushnikov
authors.forename: Pavel O
authors.initials: PO
authors.lastname: Roumiantsev
authors.forename: Akira
authors.initials: A
authors.lastname: Ohtsuru
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Namba
authors.forename: Anatoly F
authors.initials: AF
authors.lastname: Tsyb
authors.forename: Shunichi
authors.initials: S
authors.lastname: Yamashita
mesh_terms: D000236:Adenoma / Q000209:etiology / Q000235:genetics*; D000328:Adult; D002291:Carcinoma, Papillary / Q000209:etiology / Q000235:genetics*; D016022:Case-Control Studies; D004272:DNA, Mitochondrial / Q000235:genetics / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009381:Neoplasms, Radiation-Induced / Q000209:etiology / Q000235:genetics*; D011210:Power Plants; D018788:Radioactive Hazard Release; D012426:Russia; D017384:Sequence Deletion; D013964:Thyroid Neoplasms / Q000209:etiology / Q000235:genetics*; D014455:Ukraine
chemical_list: D004272:DNA, Mitochondrial