Evidence for antibody-catalyzed ozone formation in bacterial killing and inflammation.
Recently, we showed that antibodies catalyze the generation of hydrogen peroxide (H2O2) from singlet molecular oxygen (1O2*) and water. Here, we show that this process can lead to efficient killing of bacteria, regardless of the antigen specificity of the antibody. H2O2 production by antibodies alone was found to be not sufficient for bacterial killing. Our studies suggested that the antibody-catalyzed water-oxidation pathway produced an additional molecular species with a chemical signature similar to that of ozone. This species is also generated during the oxidative burst of activated human neutrophils and during inflammation. These observations suggest that alternative pathways may exist for biological killing of bacteria that are mediated by potent oxidants previously unknown to biology.
Wentworth
Paul
P
Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
McDunn
Jonathan E
JE
Wentworth
Anita D
AD
Takeuchi
Cindy
C
Nieva
Jorge
J
Jones
Teresa
T
Bautista
Cristina
C
Ruedi
Julie M
JM
Gutierrez
Abel
A
Janda
Kim D
KD
Babior
Bernard M
BM
Eschenmoser
Albert
A
Lerner
Richard A
RA
D000818:Animals; D017151:Antibodies, Catalytic / Q000276:immunology / Q000378:metabolism*; D001183:Arthus Reaction / Q000276:immunology* / Q000378:metabolism; D001770:Blood Bactericidal Activity; D002374:Catalase / Q000378:metabolism; D002384:Catalysis; D004926:Escherichia coli / Q000276:immunology*; D006415:Hematoporphyrins / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007203:Indigo Carmine / Q000378:metabolism; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D018375:Neutrophil Activation; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*; D010084:Oxidation-Reduction; D010126:Ozone / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D016897:Respiratory Burst; D026082:Singlet Oxygen / Q000378:metabolism; D014466:Ultraviolet Rays; D014867:Water / Q000378:metabolism
D017151:Antibodies, Catalytic; D006415:Hematoporphyrins; D014867:Water; D026082:Singlet Oxygen; D010126:Ozone; D006861:Hydrogen Peroxide; D007203:Indigo Carmine; D002374:Catalase