Survey of porcine rotavirus G and P genotype in Poland and the United States using RT-PCR.
Porcine rotaviruses are a common cause of gastroenteritis. Several serotypes have been detected based on the two surface proteins VP4 (P-types) and VP7 (G-types). However, limited studies have been performed on the relative frequency of rotavirus types in diarrhetic pigs primarily because of the lack of availability of suitable methods. In this study, we describe a reverse transcriptase-polymerase chain reaction (RT-PCR) method for the typing of P and G types of rotavirus. This method allowed to detect G and P types in 96.8 and 87.1% of isolates collected in the United States, respectively and in 54.5 and 38.6% of isolates collected in Poland, respectively. Within the US specimens the G3, G4, G5, G9 and G10 types were detected in combination with P6 and P7 types while among Polish specimens only G3, G4 and G5 types in combination with P6 and P7 types were identified. In both instances the G4 and G5 were the most prevalent types. These studies show that a RT-PCR typing method is suitable for molecular epidemiological studies and that there is more diversity among porcine rotavirus than previously reported.
Winiarczyk
S
S
Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, Agriculture University, Lublin, Poland. Stwiniar@hortus.ar.lublin.pl
Paul
P S
PS
Mummidi
S
S
Panek
R
R
Gradzki
Z
Z
D000818:Animals; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics*; D005243:Feces / Q000821:virology; D005260:Female; D005838:Genotype; D008297:Male; D011044:Poland / Q000453:epidemiology; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000592:standards / Q000662:veterinary*; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000453:epidemiology*; D014481:United States / Q000453:epidemiology
D017931:DNA Primers; D004279:DNA, Viral