title: Temperature effect in the production of multiple xylanases by Aspergillus fumigatus.
abstract: This work has evaluated the temperature effect in the production of multiple xylanases by a locally isolated strain of Aspergillus fumigatus Fresenius. Three isoenzymes, identified as xylanases I, II, and III with apparent molecular weight of 45.7 KDa, 39.8 KDa and 18.2 KDa, respectively, were produced in cultures developed at 30 degrees C and at 42 degrees C. The pattern of distribution of xylanase activity among the three isoenzymes was greatly affected by the growth temperature: at 30 degrees C, the total xylanase activity was distributed homogeneously among the three enzymes, while at 42 degrees C, the total xylanase activity was mainly due to the fractions with the highest MW (I and II) and the xylanase III was a minor component.
authors.affiliation: Departamento de Bioquimica, Universidade Estadual de Maringá, 87.020-900, Maringá, PR, Brazil.
authors.forename: Veridiana
authors.initials: V
authors.lastname: Lenartovicz
authors.forename: Cristina Giatti
authors.initials: CG
authors.lastname: Marques de Souza
authors.forename: Fabiana Guillen
authors.initials: FG
authors.lastname: Moreira
authors.forename: Rosane Marina
authors.initials: RM
authors.lastname: Peralta
mesh_terms: D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000378:metabolism; D018554:Avena / Q000166:cytology / Q000378:metabolism; D002482:Cellulose / Q000145:classification / Q000378:metabolism; D002850:Chromatography, Gel; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000145:classification / Q000378:metabolism; D013696:Temperature; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000096:biosynthesis* / Q000145:classification
chemical_list: D003470:Culture Media; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase