Effect of streptozotocin-induced diabetes mellitus on alveolar bone deposition in the rat.
Effects of diabetes on alveolar bone remodelling were assessed by quantitative histology and a chronological lead-labelling technique. Experimental diabetes was induced by a single dose of 40 mg/kg of streptozotocin. Remodelling of the alveolar wall surrounding the root of mandibular first molar was studied in control rats fed ad libitum, and in diabetic and insulin-treated diabetic rats 24 days after the induction of diabetes. The volumes of bone formation on the mesial side of the alveolar wall were evaluated over a 10-day period by chronological lead-labelling and computer image analysis. For a histometric measure of bone-resorption, the number of osteoclasts along the distal surface of the alveolar wall was counted. The volume of bone formed and the number of osteoclasts were significantly lower in the diabetic rats than in the controls, but insulin treatment of diabetic rats normalised these histomorphometric measures of bone turnover. These results demonstrate that streptozotocin-induced diabetes mellitus reduces the rate of bone turnover in the alveolar wall surrounding the root, which reduction is corrected by treatment with insulin.
Mishima
Naoyuki
N
Department of Oral and Maxillofacial Surgery I, Graduate School of Dentistry, The Nippon Dental University, Fujimi, Tokyo 102-8159, Japan.
Sahara
Noriyuki
N
Shirakawa
Masayori
M
Ozawa
Hidehiro
H
D016301:Alveolar Bone Loss / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D000539:Alveolar Process / Q000473:pathology / Q000503:physiopathology*; D000818:Animals; D016723:Bone Remodeling; D002452:Cell Count; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000473:pathology / Q000503:physiopathology; D005269:Femur / Q000503:physiopathology; D007004:Hypoglycemic Agents / Q000627:therapeutic use; D007328:Insulin / Q000627:therapeutic use; D008297:Male; D008336:Mandibular Diseases / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D010010:Osteoclasts / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
D007004:Hypoglycemic Agents; D007328:Insulin; D013311:Streptozocin