title: Association of MHC class I and class II gene polymorphisms with vaccine or challenge response to Salmonella enteritidis in young chicks.
abstract: Salmonella enteritidis (SE) is a worldwide source of salmonellosis in humans, caused mainly by consumption of contaminated poultry products. The major histocompatibility complex (MHC) class I and class II molecules, which function as antigen-presentation structures, are involved in both macrophage and dendritic cell immune response to Salmonella and may, therefore, play an important role in host resistance to infections. The chicken MHC class I and class II were investigated as candidate genes for immune response to SE. We characterized the complete MHC class I cDNA sequences for an outbred broiler line and four diverse highly inbred lines: two MHC-congenic Leghorn (G-B2 and G-B1), one Egyptian Fayoumi (M15.2), and one Spanish (Sp21.1), to define the allelic sequences within these lines, so that we might study the association between particular MHC polymorphisms and response to SE. The F1 offspring of outbred broiler sires crossed with three inbred lines (G-B1, G-B2, and Fayoumi) were evaluated as young chicks for either bacterial load in spleen and cecum after pathogenic SE inoculation or antibody level after SE vaccination. Alleles defined by a Lys(148)-->Met(148) polymorphism in the MHC class I alpha(2) domain were associated with spleen bacterial load after SE challenge. These results suggest that particular MHC haplotypes may contribute to control of responses to SE, and that particular polymorphisms may serve as markers for genetic resistance to SE in the chicken.
authors.affiliation: 2255 Kildee Hall, Department of Animal Science, Iowa State University, Ames, IA 50011-3150, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Marcia M
authors.initials: MM
authors.lastname: Miller
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Lamont
mesh_terms: D000818:Animals; D017849:Animals, Inbred Strains; D021461:Animals, Outbred Strains; D002645:Chickens / Q000235:genetics* / Q000276:immunology; D018076:DNA, Complementary; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D005838:Genotype; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D017434:Protein Structure, Tertiary; D022562:Salmonella Vaccines / Q000276:immunology*; D012477:Salmonella enteritidis / Q000276:immunology*
chemical_list: D018076:DNA, Complementary; D015395:Histocompatibility Antigens Class I; D022562:Salmonella Vaccines