title: Study of chaperone-like activity of human haptoglobin: conformational changes under heat shock conditions and localization of interaction sites.
abstract: With respect to the mechanism of chaperone-like activity, we examined the behavior of haptoglobin under heat shock conditions. Secondary structure changes during heat treatment were followed by circular dichroism, Raman and infrared spectroscopy. A model of the haptoglobin tetramer, based on its sequence homology with serine proteases and the CCP modules, has been proposed. Sequence regions responsible for the chaperone-like activity were not fully identical with the region that takes part in formation of the hemoglobin-haptoglobin complex. We can postulate the presence of at least two different chaperone-binding sites on each haptoglobin heavy chain.
authors.affiliation: Institute of Physical Biology, Zámek 136, CZ-37333 Nové Hrady, Czech Republic
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Ettrich
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Brandt
authors.forename: Vladimír
authors.initials: V
authors.lastname: Kopecký
authors.forename: Vladimír
authors.initials: V
authors.lastname: Baumruk
authors.forename: Katerina
authors.initials: K
authors.lastname: Hofbauerová
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Pavlícek
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002374:Catalase / Q000737:chemistry; D006242:Haptoglobins / Q000737:chemistry* / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012997:Solvents / Q000737:chemistry; D013057:Spectrum Analysis; D013816:Thermodynamics; D017186:Titrimetry
chemical_list: D006242:Haptoglobins; D006360:Heat-Shock Proteins; D006454:Hemoglobins; D018832:Molecular Chaperones; D012997:Solvents; D002374:Catalase