Influence of TRAIL gene on biomechanical properties of the human leukemic cell line Jurkat.
We cloned the cDNA fragment of human TNF-related apoptosis inducing ligand (TRAIL) into RevTet-On, a Tet-regulated and high-level gene expression system. Making use of the TRAIL gene expression system in Jurkat as a cell model, we studied the influence of TRAIL gene on the biomechanics properties of Jurkat through measuring changes of cellular biomechanics properties before and after the TRAIL gene expression, which was induced by adding tetracycline derivative doxycycline (Dox). The results indicated that the TRAIL gene expression led to significant changes in cellular biomechanics properties. The osmotic fragility increased and the cell stiffness increased after the expression of TRAIL gene. Thus, the apoptosis-inducing TRAIL gene caused significant changes in the biomechanics properties of Jurkat cells.
Yao
Weijuan
W
School of Basic Medical Sciences, Department of Biophysics, Peking University, 100083, Beijing, China.
Chen
Kai
K
Wang
Xinjuan
X
Xie
Lide
L
Wen
Zongyao
Z
Yan
Zongyi
Z
Chien
Shu
S
D017209:Apoptosis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins; D001696:Biomechanical Phenomena; D003001:Cloning, Molecular; D015536:Down-Regulation / Q000502:physiology; D004318:Doxycycline / Q000008:administration & dosage; D004548:Elasticity; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells / Q000145:classification / Q000187:drug effects / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000502:physiology*; D009997:Osmotic Pressure; D012680:Sensitivity and Specificity; D013314:Stress, Mechanical; D053221:TNF-Related Apoptosis-Inducing Ligand; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*; D014783:Viscosity
D051017:Apoptosis Regulatory Proteins; D008562:Membrane Glycoproteins; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D014409:Tumor Necrosis Factor-alpha; D004318:Doxycycline