Structure-activity relationship on fungal laccase from Rigidoporus lignosus: a Fourier-transform infrared spectroscopic study.
The structure and thermal stability of a laccase from Rigidoporus lignosus (Rl) was analysed by Fourier-transform infrared (FT-IR) spectroscopy. The enzyme was depleted of copper atoms, then part of the apoenzyme was re-metalled and these two forms of the protein were analysed as well. The enzymatic activity, lost by the removal of copper atoms, was restored in the re-metalled apoenzyme and resulted similar to that of native protein. The infrared data indicated that the enzyme contains a large amount of beta-sheets and a small content of alpha-helices, and it displayed a marked thermostability showing the T(m) at 92.5 degrees C. The apoenzyme and the re-metalled apoenzyme did not show remarkable differences in the secondary structure with respect to the native protein, but the thermal stability of the apoenzyme was dramatically reduced showing a T(m) close to 72 degrees C, while the re-metalled protein displayed the T(m) at 90 degrees C. These data indicate that copper atoms, beside their role in catalytic activity, play also an important role on the stabilisation of the structure of Rl laccase. About 35% of the polypeptide chain is buried and/or constitutes a particular compact structure, which, beside copper atoms, is probably involved in the high thermal stability of the protein. Another small part of the structure is particularly sensitive to high temperatures and it could be the cause of the loss of enzymatic activity when the temperature is raised above 45-50 degrees C.
Ragusa
Santa
S
Department of Chemical Sciences, University of Catania, 95125 Catania, Italy.
Cambria
Maria Teresa
MT
Pierfederici
Francesco
F
Scirè
Andrea
A
Bertoli
Enrico
E
Tanfani
Fabio
F
Cambria
Antonio
A
D004578:Electron Spin Resonance Spectroscopy; D004795:Enzyme Stability; D006358:Hot Temperature; D007700:Kinetics; D042845:Laccase; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism*; D020072:Polyporales / Q000201:enzymology*; D011489:Protein Denaturation; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013816:Thermodynamics
D010088:Oxidoreductases; D042845:Laccase