Tumor necrosis factor-producing activity of wogonin in RAW 264.7 murine macrophage cell line.
Wogonin from Scutellaria baicalensis, was demonstrated to increase nitric oxide (NO) in the murine macrophage cell line RAW 264.7. It is our aim to investigate the modulatory effect of wogonin on tumor necrosis factor-alpha (TNF-alpha) gene expression. By using RAW 264.7 as an in vitro model, the effects of wogonin on inducible nitric oxide synthase (NOS2) and TNF-alpha gene expression were evaluated by ELISA and reverse transcribed polymerase chain reaction (RT-PCR). Aspirin and H7 were used to determine the possible signal transduction pathways. The results showed that wogonin at the concentration of 10 (-5) M and 10 (-6) M up-regulated NOS2 gene expression in RAW 264.7 cells. Besides, wogonin up-regulated the gene expression of TNF-alpha, in terms of TNF-alpha secretion and transcription, in a dose dependent manner. The fact that aspirin but not H7 blocks the enhancing effect suggests that NF-kappaB might be involved in wogonin-enhanced TNF-alpha gene expression. We conclude that a low concentration of wogonin up-regulates NOS2 and TNF-alpha gene expression through NF-kappaB pathway.
Chiu
Jen-Hwey
JH
Lay
Ing-Shiow
IS
Su
Ming-Yuan
MY
Chiu
Hsiu-Li
HL
Chiu
Ai-Chin
AC
Lui
Wing-Yiu
WY
Wu
Chew-Wun
CW
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line / Q000187:drug effects; D017931:DNA Primers; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D044950:Flavanones; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology*; D005786:Gene Expression Regulation; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D019001:Nitric Oxide Synthase / Q000187:drug effects*; D052247:Nitric Oxide Synthase Type II; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D031363:Scutellaria; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects*
D017931:DNA Primers; D044950:Flavanones; D005419:Flavonoids; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C085514:wogonin