Parallel analysis with optically gated sample introduction on a multichannel microchip.
As an alternative to the T-type injection on microchips, optically gated sample introduction previously has been demonstrated to provide fast, serial, and reproducible injections on a single-channel microchip. Here, the ability to perform high throughput, multichannel analysis with optically gated sample introduction is described using a voice coil actuator. The microchip is fixed on a stage, which moves back and forth via the voice coil actuator, scanning two laser beams across the channels on the microchip. For parallel analysis on a multichannel microchip, both the gating beam and the probe beam are scanned at 10 Hz to perform multiple injections and parallel detection. Simultaneous, fast separations of 4-choloro-7-nitrobenzofurazan (NBD)-labeled amino acids are demonstrated in multiple channels on a microchip. Serial separations of different samples in multiple channels are also reported. Optically gated sample introduction on multiple, parallel channels shows the potential for high-speed, high-throughput separations that are easily automated by using a single electronic shutter.
Xu
Hongwei
H
Department of Chemistry, Pennsylvania State University, University Park 16802, USA.
Roddy
Thomas P
TP
Lapos
Julie A
JA
Ewing
Andrew G
AG
D009327:4-Chloro-7-nitrobenzofurazan / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification; D019075:Electrophoresis, Capillary; D007834:Lasers; D008904:Miniaturization; D055095:Optics and Photonics / Q000295:instrumentation; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000596:Amino Acids; D009327:4-Chloro-7-nitrobenzofurazan