title: Characterisation of reversed-phase liquid chromatographic columns by chromatographic tests. Evaluation of 36 test parameters: repeatability, reproducibility and correlation.
abstract: The European Pharmacopoeia (Ph. Eur.) or other official compendia give only a general description of the stationary phase in the description of a liquid chromatographic method. Therefore the selection of a column giving suitable selectivity presents difficulties. Earlier, a test procedure was proposed that allows measurement of a number of parameters which are reported to be representative for stationary phase characteristics. This paper describes how the test procedure was applied on 69 RP-LC C18 columns. Chromatographic parameters obtained as test results were evaluated, and their repeatability, reproducibility and correlation were examined.
authors.affiliation: Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Katholieke Universiteit Leuven, Van Evenstraat 4, B-3000 Leaven, Belgium.
authors.forename: Dóra
authors.initials: D
authors.lastname: Visky
authors.forename: Yvan Vander
authors.initials: YV
authors.lastname: Heyden
authors.forename: Tímea
authors.initials: T
authors.lastname: Iványi
authors.forename: Peggy
authors.initials: P
authors.lastname: Baten
authors.forename: Jacques
authors.initials: J
authors.lastname: De Beer
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Kovács
authors.forename: Béla
authors.initials: B
authors.lastname: Noszál
authors.forename: Eugène
authors.initials: E
authors.lastname: Roets
authors.forename: Désiré L
authors.initials: DL
authors.lastname: Massart
authors.forename: Jos
authors.initials: J
authors.lastname: Hoogmartens
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet