Mouse plasmacytoid cells: long-lived cells, heterogeneous in surface phenotype and function, that differentiate into CD8(+) dendritic cells only after microbial stimulus.
The CD45RA(hi)CD11c(int) plasmacytoid predendritic cells (p-preDCs) of mouse lymphoid organs, when stimulated in culture with CpG or influenza virus, produce large amounts of type I interferons and transform without division into CD8(+)CD205(-) DCs. P-preDCs express CIRE, the murine equivalent of DC-specific intercellular adhesion molecule 3 grabbing nonintegrin (DC-SIGN). P-preDCs are divisible by CD4 expression into two subgroups differing in turnover rate and in response to Staphylococcus aureus. The kinetics of bromodeoxyuridine labeling and the results of transfer to normal recipient mice indicate that CD4(-) p-preDCs are the immediate precursors of CD4(+) p-preDCs. Similar experiments indicate that p-preDCs are normally long lived and are not the precursors of the short-lived steady-state conventional DCs. However, in line with the culture studies on transfer to influenza virus-stimulated mice the p-preDCs transform into CD8(+)CD205(-) DCs, distinct from conventional CD8(+)CD205(+) DCs. Hence as well as activating preexistant DCs, microbial infection induces a wave of production of a new DC subtype. The functional implications of this shift in the DC network remain to be determined.
O'Keeffe
Meredith
M
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia. okeefe@wehi.edu.au
Hochrein
Hubertus
H
Vremec
David
D
Caminschi
Irina
I
Miller
Joanna L
JL
Anders
E Margot
EM
Wu
Li
L
Lahoud
Mireille H
MH
Henri
Sandrine
S
Scott
Bernadette
B
Hertzog
Paul
P
Tatarczuch
Lilliana
L
Shortman
Ken
K
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D005260:Female; D008221:Lymphoid Tissue / Q000166:cytology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009975:Orthomyxoviridae / Q000276:immunology*; D010641:Phenotype; D013211:Staphylococcus aureus / Q000276:immunology*