Novel two-band ratiometric fluorescence probes with different location and orientation in phospholipid membranes.
3-hydroxyflavone (3-HF) derivatives are very attractive fluorescence sensors due to their ability to respond to small changes in their microenvironment via a dramatic alteration of the relative intensities of their two well-separated emission bands. We developed fluorescence probes with locations at different depths and orientations of 3-HF moiety in the phospholipid bilayer, which determine their fluorescence behavior. While the spectral shifts of the probes correlate with their binding site polarity, the intensity ratio is a complex parameter that is also sensitive to the local hydration. We demonstrate that even the deeply located probes sense this hydration effect, which can be modulated by the charge of the lipid heads and is anisotropic with respect to the bilayer plane. Thus the two-band ratiometric fluorescence probes can provide multiparametric information on the properties of lipid membranes at different depths.
Klymchenko
Andrey S
AS
TUBITAK Research Institute for Genetic Engineering and Biotechnology, Gebze-Kocaeli 41470, Turkey.
Duportail
Guy
G
Ozturk
Turan
T
Pivovarenko
Vasyl G
VG
Mély
Yves
Y
Demchenko
Alexander P
AP
D005419:Flavonoids / Q000138:chemical synthesis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D055672:Static Electricity; D014867:Water / Q000737:chemistry
D005419:Flavonoids; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D014867:Water; C017251:1,2-oleoylphosphatidylcholine; C041477:3-hydroxyflavone