Lipids are co-eluted with immunoglobulins G during purification by recombinant streptococcal protein G affinity chromatography.
The efficiency of recombinant streptococcal protein G (rec-spG) affinity column chromatography in purifying immunoglobulins G (IgG) from lipids has been studied, with particular reference to IgG fractions from bronchoalveolar lavage (BAL) fluid and serum samples from different sources. It was found that the IgG fractions purified by rec-spG affinity column chromatography also contained cholesterol and phospholipids.
Kitsiouli
Eirini
E
Laboratoire de Biogenèse Membranaire CNRS-UMR 5544, Université Victor Segalen, Bordeaux 2, France. me00157@cc.uoi.gr
Lekka
Marilena E
ME
Nakos
George
G
Cassagne
Claude
C
Maneta-Peyret
Lilly
L
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002784:Cholesterol / Q000097:blood; D002846:Chromatography, Affinity / Q000379:methods* / Q000592:standards; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification*; D008055:Lipids / Q000097:blood / Q000302:isolation & purification*; D010743:Phospholipids / Q000097:blood; D011817:Rabbits
D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D007074:Immunoglobulin G; D008055:Lipids; D010743:Phospholipids; D002784:Cholesterol