Structure determination of a cocaine hydrolytic antibody from a pseudomerohedrally twinned crystal.
Few examples of pseudomerohedrally twinned macromolecular crystals have been described in the literature. This unusual phenomenon arises when a fortuitous unit-cell geometry makes it possible for twinning to occur in a space group that ordinarily does not allow twinning. Here, the crystallization, structure determination and refinement of the cocaine hydrolytic antibody 15A10 at 2.35 A resolution are described. The crystal belongs to space group P2(1), with two molecules in the asymmetric unit and unit-cell parameters a = 37.5, b = 108.4, c = 111.3 A and beta fortuitously near 90 degrees; the refined twinning fraction is alpha = 0.43. Interestingly, the non-crystallographic symmetry (NCS) and twin operators are nearly parallel, which appears to be a relatively frequent situation in protein crystals twinned by merohedry or pseudomerohedry.
Larsen
Nicholas A
NA
The Department of Molecular Biology and Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California, USA.
Heine
Andreas
A
de Prada
Paloma
P
Redwan
El-Rashdy
el-R
Yeates
Todd O
TO
Landry
Donald W
DW
Wilson
Ian A
IA
D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D003042:Cocaine / Q000276:immunology / Q000378:metabolism*; D006868:Hydrolysis; D011487:Protein Conformation
D000906:Antibodies; D003042:Cocaine