[The effect of simulated weightlessness on the function of spleen lymphocytes and the expression of c-fos proto-oncogene in tail-suspended mice].
Objective. To investigate the effect of simulated weightlessness on proliferation of spleen lymphocyte, production of interleukin-2 and expression of c-fos proto-oncogene in mice. Method. Mice were tail-suspended for 7 or 14 days to simulate the effect of weightlessness; proliferation of lymphocyte was measured by MTT method, production of IL-2 was determined by biological activity and expression of c-fos proto-oncogene was determined by dot blotting. Result. As compared with the control, the proliferation of spleen lymphocyte and the production of IL-2 were significantly decreased in 14 d suspended group, but the expression of c-fos proto-oncogene [correction of oncogen] was increased in both 7 d and 14 d suspended groups. Conclusion. This result suggested that simulated weightlessness could interfere with the genes regulating the lymphocyte.
Zhang
H
H
Institute of Space Medico-Engineering, Beijing, China.
Hu
P
P
Wen
X L
XL
Huang
B
B
D000818:Animals; D015870:Gene Expression; D016762:Genes, fos / Q000235:genetics*; D019417:Hindlimb Suspension; D007376:Interleukin-2 / Q000378:metabolism*; D008214:Lymphocytes / Q000502:physiology*; D051379:Mice; D011519:Proto-Oncogenes; D013154:Spleen / Q000166:cytology*; D018474:Weightlessness Simulation
D007376:Interleukin-2