A comparative map of bovine chromosome 25 with human chromosomes 7 and 16.
A comparative genome map is necessary for the implementation of comparative positional candidate gene cloning in cattle. We have developed a medium density comparative gene map of bovine chromosome 25 (BTA25). A radiation hybrid (RH) panel was used to map nine microsatellites and nine genes. Eight of the nine comparative loci were also mapped by FISH. These results were combined with data from published articles to create a comprehensive comparative map of BTA25 with human chromosomes 7 (HSA7) and 16 (HSA16). This map should facilitate the cloning of genes of interest on bovine chromosome 25.
Antoniou
E
E
University of Missouri, S 140 Animal Sciences Research Center, Columbia, MO 65211, USA. AntoniouE@Missouri.edu
Gallagher
D
D
Taylor
J
J
Davis
S
S
Womack
J
J
Grosz
M
M
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics*; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D033481:Chromosomes, Mammalian / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D018895:Microsatellite Repeats; D040641:Quantitative Trait Loci; D013045:Species Specificity
D017931:DNA Primers