Induction of neural differentiation by electrically stimulated gene expression of NeuroD2.
Regulation of cell differentiation is an important assignment for cellular engineering. One of the techniques for regulation is gene transfection into undifferentiated cells. Transient expression of NeuroD2, one of neural bHLH transcription factors, converted mouse N1E-115 neuroblastoma cells into differentiated neurons. The regulation of neural bHLH expression should be a novel strategy for cell differentiation. In this study, we tried to regulate neural differentiation by NeuroD2 gene inserted under the control of heat shock protein-70 (HSP) promoter, which can be activated by electrical stimulation. Mouse neuroblastoma cell line, N1E-115, was stably transfected with expression vector containing mouse NeuroD2 cDNA under HSP promoter. Transfected cells were cultured on the electrode surface and applied electrical stimulation. After stimulation, NeuroD2 expression was induced, and transfected cells adopt a neuronal morphology at 3 days after stimulation. These results suggest that neural differentiation can be induced by electrically stimulated gene expression of NeuroD2.
Mie
Masayasu
M
Department of Biological Information, Graduate school of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Endoh
Tamaki
T
Yanagida
Yasuko
Y
Kobatake
Eiry
E
Aizawa
Masuo
M
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D002454:Cell Differentiation / Q000235:genetics / Q000528:radiation effects; D002460:Cell Line; D003001:Cloning, Molecular; D004558:Electric Stimulation; D004574:Electromagnetic Fields; D005786:Gene Expression Regulation / Q000528:radiation effects*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism / Q000528:radiation effects; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology* / Q000503:physiopathology; D009479:Neuropeptides / Q000096:biosynthesis* / Q000235:genetics*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics*; D014162:Transfection / Q000379:methods
D051792:Basic Helix-Loop-Helix Proteins; D018840:HSP70 Heat-Shock Proteins; D009479:Neuropeptides; D014157:Transcription Factors; C098995:Neurod2 protein, mouse