title: Increased expression of phosphatase and tensin homolog in reactive astrogliosis following intracerebroventricular kainic acid injection in mouse hippocampus.
abstract: A phosphatase and tensin homolog (PTEN) has been known to play multiple biological roles. However, role of PTEN in astrocyte activation is not clear yet. In the present study, the expression pattern of PTEN in the process of reactive gliosis was immunohistochemically examined in intracerebroventricular (i.c.v.) injected kainic acid mouse hippocampus. Mice were grouped into three; 30 min, 1 day and 7 days after kainic acid i.c.v. injection. Thirty minutes after kainic acid i.c.v. injection, astrocytes were activated and PTEN was weakly expressed in immature astrocytes. Seven days after kainic acid i.c.v. injection, PTEN expression was decreased in highly activated astrocytes showing extensively spindled shape. Immunofluorescence double labeling experiment showed that PTEN was expressed in glial fibrillary acidic protein-positive astrocytes. These findings suggest that PTEN might have a role in early stage of reactive astrogliosis in vivo.
authors.affiliation: Department of Anatomy, Seoul National University College of Medicine and Institute of Neuroscience, Medical research Center, Seoul National University, Yongon-dong 28, Chongno-gu, Seoul 110-799, South Korea.
authors.forename: Jaeyoung
authors.initials: J
authors.lastname: Cho
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Je Hoon
authors.initials: JH
authors.lastname: Seo
authors.forename: Hee-Sung
authors.initials: HS
authors.lastname: Kim
authors.forename: Jung-Gil
authors.initials: JG
authors.lastname: Ahn
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Kim
authors.forename: Sung-Vin
authors.initials: SV
authors.lastname: Yim
authors.forename: Dong-Keun
authors.initials: DK
authors.lastname: Song
authors.forename: Sa Sun
authors.initials: SS
authors.lastname: Cho
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D018690:Excitatory Amino Acid Agonists / Q000008:administration & dosage / Q000494:pharmacology*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000187:drug effects; D005911:Gliosis / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D007608:Kainic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D051059:PTEN Phosphohydrolase; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis / Q000187:drug effects*; D013997:Time Factors; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis / Q000187:drug effects*
chemical_list: D018690:Excitatory Amino Acid Agonists; D005904:Glial Fibrillary Acidic Protein; D025521:Tumor Suppressor Proteins; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; D007608:Kainic Acid