title: Cathepsin B stability, but not activity, is affected in cysteine:cystine redox buffers.
abstract: In order to test the hypothesis that the lysosomal cysteine protease cathepsin B may be redox regulated in vivo, cathepsin B activity and stability were measured in cysteine- and/or cystine-containing buffers. Cathepsin B activity in cysteine-containing buffers was similar at pH 6.0 and pH 7.0, over all thiol concentrations tested. In contrast, the stability of the enzyme was greater at pH 6.0 than at pH 7.0. This suggests that the enzyme's operational pH in vivo may be < pH 7.0. The activity of the enzyme was depressed in glutathione-containing buffers. When assessed in cysteine:cystine redox buffers (pH 6.0-7.0) cathepsin B was active over a broad redox potential range, suggesting that cathepsin B activity may not be redox regulated. However, at pH 7.0, the stability of cathepsin B decreased with increasing reduction potential and ambient cystine concentration. This suggests that the stability of the enzyme at neutral pH is dependent on redox potential, and on the presence of oxidising agents.
authors.affiliation: Department of Biochemistry, School of Molecular and Cellular Biosciences, University of Natal, Pietermaritzburg, South Africa.
authors.forename: Ché S
authors.initials: CS
authors.lastname: Pillay
authors.forename: Clive
authors.initials: C
authors.lastname: Dennison
mesh_terms: D000818:Animals; D002021:Buffers; D002401:Cathepsin B / Q000737:chemistry* / Q000378:metabolism*; D002417:Cattle; D003545:Cysteine / Q000494:pharmacology; D003553:Cystine / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D005978:Glutathione / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D016877:Oxidants / Q000494:pharmacology; D010084:Oxidation-Reduction
chemical_list: D002021:Buffers; D016877:Oxidants; D003553:Cystine; D002401:Cathepsin B; D005978:Glutathione; D003545:Cysteine