title: [11C]Choline as a potential PET marker for imaging of breast cancer athymic mice.
abstract: [11C]Choline has been evaluated as a potential positron emission tomography (PET) marker for imaging of breast cancer. The biodistribution of [11C]choline was determined at 45 min post iv injection in MCF-7's transfected with IL-1alpha implanted athymic mice and MDA-MB-435 implanted athymic mice. The results showed the uptake of [11C]choline in these tumors was high, 2.0% dose/g in MCF-7's transfected with IL-1alpha implanted mice and 1.8% dose/g in MDA-MB-435 implanted mice; the ratios of tumor/muscle (T/M) and tumor/blood (T/B) were 1.7 (T/M, MCF-7's), 2.1 (T/M, MDA-MB-435) and 6.9 (T/B, MCF-7's), 12.5 (T/B, MDA-MB-435), respectively; the tumor/muscle ratios are moderate, and the tumor/blood ratios are high. The micro-PET imaging of [11C]choline in both breast cancer athymic mice was acquired for 15 min from a MCF-7's transfected with IL-1alpha and/or MDA-MB-435 implanted mouse at 45 min post iv injection of 1 mCi of the tracer using a dedicated high resolution (<3 mm full-width at half-maximum) small FOV (field-of-view) PET imaging system, Indy-PET II scanner, developed in our laboratory, which showed the uptake of [11C]choline in MCF-7's transfected with IL-1alpha tumor or MDA-MB-435 tumor implanted in a nude athymic mouse. These results suggest that [11C]choline may be a potential PET breast cancer imaging agent.
authors.affiliation: Department of Radiology, Indiana University School of Medicine, Indianapolis, IN 46202, USA. qzheng@iupui.edu
authors.forename: Qi-Huang
authors.initials: QH
authors.lastname: Zheng
authors.forename: K Lee
authors.initials: KL
authors.lastname: Stone
authors.forename: Bruce H
authors.initials: BH
authors.lastname: Mock
authors.forename: Kathy D
authors.initials: KD
authors.lastname: Miller
authors.forename: Xiangshu
authors.initials: X
authors.lastname: Fei
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.forename: Ji-Quan
authors.initials: JQ
authors.lastname: Wang
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Glick-Wilson
authors.forename: George W
authors.initials: GW
authors.lastname: Sledge
authors.forename: Gary D
authors.initials: GD
authors.lastname: Hutchins
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D002250:Carbon Radioisotopes / Q000737:chemistry / Q000493:pharmacokinetics; D002794:Choline / Q000138:chemical synthesis / Q000493:pharmacokinetics*; D005240:Feasibility Studies; D051379:Mice; D009368:Neoplasm Transplantation; D009928:Organ Specificity; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000493:pharmacokinetics; D014018:Tissue Distribution; D014055:Tomography, Emission-Computed / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D002250:Carbon Radioisotopes; D019275:Radiopharmaceuticals; D002794:Choline