title: The effects of electrode position on the excitability of rat atria during postnatal development.
abstract: Cardiac excitability is determined by the direction of the electric field, which is defined by the positioning of electrodes. However, important morphological and physiological modifications that happen during the postnatal development of the heart may affect the cardiac threshold. In this work we have evaluated the effect of electrode positioning on the excitability threshold of isolated Wistar rat atria (left auricles) during postnatal development. This was performed by determining the parameters of strength-duration curves for stimuli (rheobase, chronaxie and normalized minimum pulse energy) of atria from rats at ages (days) 5, 15, 30, 60, 90 and 120. These parameters were determined using electric field stimulation in four different orientations (apex-base, base-apex, left-right and right-left). Atrial rheobase decreased by 1.5- to 4-fold with animal age and was altered by electric field orientation in a diversified way, whereas atrial chronaxie increased only with animal age. The minimum pulse energy decreased two- to nine-fold with ageing. This was mainly due to rheobase dependence with electric field direction. We showed that the appropriate cardiac stimulation depends on the effects of three combined factors (pulse parameters, electrode position and animal age) on the atrial tissue excitability.
authors.affiliation: Laboratório de Eletrofisiologia Cardíaca, Núcleo de Pesquisas Tecnológicas, Universidade de Mogi das Cruzes, Avenida Dr Cândido Xavier de Almeida e Souza 200, Mogi das Cruzes, SP, 08780-911, Brazil. mgodoy@umc.br
authors.forename: Carlos Marcelo
authors.initials: CM
authors.lastname: Gurjão de Godoy
authors.forename: Kleber
authors.initials: K
authors.lastname: de Magalhães Galvão
authors.forename: Tatiana
authors.initials: T
authors.lastname: de Almeida Bacarin
authors.forename: Gisela Rosa
authors.initials: GR
authors.lastname: Franco
mesh_terms: D000818:Animals; D004558:Electric Stimulation / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004574:Electromagnetic Fields; D006321:Heart / Q000033:anatomy & histology / Q000254:growth & development* / Q000502:physiology*; D006325:Heart Atria / Q000033:anatomy & histology / Q000254:growth & development; D008297:Male; D051381:Rats; D017208:Rats, Wistar