Gene discovery in the freshwater fish parasite Trypanosoma carassii: identification of trans-sialidase-like and mucin-like genes.
A total of 1,921 expressed sequence tags (ESTs) were obtained from bloodstream trypomastigotes of Trypanosoma carassii, a parasite of economic importance due to its high prevalence in fish farms. Analysis of the data set allowed us to identify a trans-sialidase (TS)-like gene and three ESTs coding for putative mucin-like genes. TS activity was detected in cell extracts of bloodstream trypomastigotes. We have also used the sequence information obtained to identify genes that have not been previously described in trypanosomatids. (Additional information on these ESTs can be found at http://genoma.unsam.edu.ar/projects/tca.)
Agüero
Fernán
F
Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús, Universidad Nacional de General San Martín-CONICET, San Martín, 1650 Buenos Aires, Argentina.
Campo
Vanina
V
Cremona
Laura
L
Jäger
Adriana
A
Di Noia
Javier M
JM
Overath
Peter
P
Sánchez
Daniel O
DO
Frasch
Alberto Carlos
AC
D000595:Amino Acid Sequence; D000818:Animals; D020224:Expressed Sequence Tags; D005393:Fish Diseases / Q000469:parasitology*; D005399:Fishes / Q000469:parasitology; D005618:Fresh Water; D006023:Glycoproteins; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics*; D009439:Neuraminidase / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D014345:Trypanosoma / Q000201:enzymology* / Q000235:genetics; D014352:Trypanosomiasis / Q000469:parasitology / Q000662:veterinary*
D006023:Glycoproteins; D009077:Mucins; D015800:Protozoan Proteins; C069715:trans-sialidase; D009439:Neuraminidase