title: Evaluation of anti-parvovirus B19 activity in sera by assay using quantitative polymerase chain reaction.
abstract: Human parvovirus B19 (B19) infects cells of erythroid lineage. Production of neutralizing antibodies (Abs) is indispensable for recovery from B19-related disease state. In this study, we used a convenient method to measure neutralizing activities in human sera by using a real-time quantitative PCR based assay. Erythroid cell line KU812Ep6 was incubated with test sera before infection with B19 virus. The copy number of B19-DNA in cultures was decreased in the presence of the sera from patients who recovered from acute B19 infection, whereas no decrease in B19-DNA was in cultures incubated with sera from healthy volunteers who had no B19 infection. The decrease in B19-DNA copy number was calculated and the inhibition percentage was expressed as neutralizing activity to B19. A clinical study showed that the levels of neutralizing ability were high in patients who recovered soon after acute B19 infection, but were low in some patients with a prolonged clinical course for recovery from B19 infection. This method is simple and convenient compared with methods described previously, showing its usefulness to evaluate the neutralizing activity to B19.
authors.affiliation: Department of Molecular Diagnostics, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Saito
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Munakata
authors.forename: Yi
authors.initials: Y
authors.lastname: Fu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fujii
authors.forename: Takao
authors.initials: T
authors.lastname: Kodera
authors.forename: Eiji
authors.initials: E
authors.lastname: Miyagawa
authors.forename: Keiko
authors.initials: K
authors.lastname: Ishii
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D016732:Parvovirus B19, Human / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D000914:Antibodies, Viral