Increased expression of phosphatase and tensin homolog in reactive astrogliosis following intracerebroventricular kainic acid injection in mouse hippocampus.
A phosphatase and tensin homolog (PTEN) has been known to play multiple biological roles. However, role of PTEN in astrocyte activation is not clear yet. In the present study, the expression pattern of PTEN in the process of reactive gliosis was immunohistochemically examined in intracerebroventricular (i.c.v.) injected kainic acid mouse hippocampus. Mice were grouped into three; 30 min, 1 day and 7 days after kainic acid i.c.v. injection. Thirty minutes after kainic acid i.c.v. injection, astrocytes were activated and PTEN was weakly expressed in immature astrocytes. Seven days after kainic acid i.c.v. injection, PTEN expression was decreased in highly activated astrocytes showing extensively spindled shape. Immunofluorescence double labeling experiment showed that PTEN was expressed in glial fibrillary acidic protein-positive astrocytes. These findings suggest that PTEN might have a role in early stage of reactive astrogliosis in vivo.
Cho
Jaeyoung
J
Department of Anatomy, Seoul National University College of Medicine and Institute of Neuroscience, Medical research Center, Seoul National University, Yongon-dong 28, Chongno-gu, Seoul 110-799, South Korea.
Lee
Sang-Hyun
SH
Seo
Je Hoon
JH
Kim
Hee-Sung
HS
Ahn
Jung-Gil
JG
Kim
Sung-Soo
SS
Yim
Sung-Vin
SV
Song
Dong-Keun
DK
Cho
Sa Sun
SS
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D018690:Excitatory Amino Acid Agonists / Q000008:administration & dosage / Q000494:pharmacology*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000187:drug effects; D005911:Gliosis / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D007608:Kainic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D051059:PTEN Phosphohydrolase; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis / Q000187:drug effects*; D013997:Time Factors; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis / Q000187:drug effects*
D018690:Excitatory Amino Acid Agonists; D005904:Glial Fibrillary Acidic Protein; D025521:Tumor Suppressor Proteins; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; D007608:Kainic Acid