Role of abscissic acid in water stress-induced antioxidant defense in leaves of maize seedlings.
Roles of abscissic acid (ABA) in water stress-induced oxidative stress were investigated in leaves of maize (Zea mays L.) seedlings exposed to water stress induced by polyethylene glycol (PEG 6000). Treatment with PEG at -0.7 MPa for 12 and 24 h led to a reduction in leaf relative water content (RWC) by 7.8 and 14.1%, respectively. Duration of the osmotic treatments is considered as mild and moderate water stress. The mild water stress caused significant increases in the generation of superoxide radical (O2-) and hydrogen peroxide (H2O2), the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) and the contents of ascorbate (ASC), reduced glutathione (GSH). The moderate water stress failed to further enhance the capacity of antioxidant defense systems, as compared to the mild water stress. The contents of catalytic Fe, which is critical for H2O2-dependent hydroxyl radical (OH) production, and the oxidized forms of ascorbate and glutathione pools, dehydroascorbate (DHA) and oxidized glutathione (GSSG), markedly increased, a significant oxidative damage to lipids and proteins took place under the moderate water stress. Pretreatment with ABA caused an obvious reduction in the content of catalytic Fe and significant increases in the activities of antioxidant enzymes and the contents of non-enzymatic antioxidants, and then significantly reduced the contents of DHA and GSSG and the degrees of oxidative damage in leaves exposed to the moderate water stress. Pretreatment with an ABA biosynthesis inhibitor, tungstate, significantly suppressed the accumulation of ABA induced by water stress, reduced the enhancement in the capacity of antioxidant defense systems, and resulted in an increase in catalytic Fe, DHA and GSSG, and oxidative damage in the water-stressed leaves. These effects were completely prevented by addition of ABA, which raised the internal ABA content. Our data indicate that ABA plays an important role in water stress-induced antioxidant defense against oxidative stress.
Jiang
Mingyi
M
Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, People's Republic of China.
Zhang
Jianhua
J
D000040:Abscisic Acid / Q000494:pharmacology*; D060387:Ascorbate Peroxidases; D001205:Ascorbic Acid / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004249:DNA Damage; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D009997:Osmotic Pressure; D010544:Peroxidases / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D017973:Tungsten Compounds / Q000494:pharmacology; D014867:Water / Q000378:metabolism*; D003313:Zea mays / Q000187:drug effects* / Q000201:enzymology
D010937:Plant Growth Regulators; D017392:Thiobarbituric Acid Reactive Substances; D017973:Tungsten Compounds; D014867:Water; D013481:Superoxides; D011092:Polyethylene Glycols; D000040:Abscisic Acid; D006861:Hydrogen Peroxide; D007501:Iron; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D005980:Glutathione Reductase; D005978:Glutathione; D001205:Ascorbic Acid; C045951:tungstate