Improved recovery of pathogenic Vibrio parahaemolyticus from oysters using colony hybridization following enrichment.
The traditional streak plating and alternative spread-plating methods were compared for detection of pathogenic Vibrio parahaemolyticus (Vp) in oyster enrichments. We found the alternative method to be more efficient: it was quicker (2d vs. 3d) and had a significantly (p < 0.05) greater detection rate than streak plating.
Nordstrom
Jessica L
JL
US Food and Drug Administration, Gulf Coast Seafood Laboratory, Post Office Box 158, Dauphin Island, AL 36528-0158, USA. jessica.nordstrom@cfsan.fda.gov
DePaola
Angelo
A
D000818:Animals; D001427:Bacterial Toxins; D015169:Colony Count, Microbial / Q000379:methods*; D003470:Culture Media; D006460:Hemolysin Proteins / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010125:Ostreidae / Q000382:microbiology*; D012680:Sensitivity and Specificity; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity
D001427:Bacterial Toxins; D003470:Culture Media; D006460:Hemolysin Proteins; C068489:thermostable direct hemolysin