Modulation of ATP-dependent chromatin-remodeling complexes by inositol polyphosphates.
Eukaryotes use adenosine triphosphate (ATP)-dependent chromatin-remodeling complexes to regulate gene expression. Here, we show that inositol polyphosphates can modulate the activities of several chromatin-remodeling complexes in vitro. Inositol hexakisphosphate (IP6) inhibits nucleosome mobilization by NURF, ISW2, and INO80 complexes. In contrast, nucleosome mobilization by the yeast SWI/SNF complex is stimulated by inositol tetrakisphosphate (IP4) and inositol pentakisphosphate (IP5). We demonstrate that mutations in genes encoding inositol polyphosphate kinases that produce IP4, IP5, and IP6 impair transcription in vivo. These results provide a link between inositol polyphosphates, chromatin remodeling, and gene expression.
Shen
Xuetong
X
Laboratory of Molecular Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4255, USA.
Xiao
Hua
H
Ranallo
Ryan
R
Wu
Wei-Hua
WH
Wu
Carl
C
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D007295:Inositol Phosphates / Q000378:metabolism*; D007296:Myo-Inositol-1-Phosphate Synthase / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins; D009707:Nucleosomes / Q000378:metabolism*; D010833:Phytic Acid / Q000378:metabolism / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
D002843:Chromatin; D004268:DNA-Binding Proteins; C086468:ISWI protein; D007295:Inositol Phosphates; D009687:Nuclear Proteins; D009707:Nucleosomes; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014157:Transcription Factors; C081651:inositol-1,4,5,6-tetrakisphosphate; C013571:inositol pentaphosphate; D010833:Phytic Acid; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D007296:Myo-Inositol-1-Phosphate Synthase