Enhanced permeability of molecular weight markers and poorly bioavailable compounds across Caco-2 cell monolayers using the absorption enhancer, zonula occludens toxin.
OBJECTIVE
Zonula occludens toxin (Zot), a protein elaborated from Vibrio cholerae, has been shown to be capable of reversibly opening tight junctions. The objective of this work was to determine the stability of Zot and to examine the permeability of a series of molecular weight hydrophilic markers and therapeutic agents in the presence of Zot.


METHODS
The transport of molecular weight markers (i.e., PEG 4000, FITC-dextran 10.000 and inulin) and therapeutic agents (i.e., acyclcovir, cyclopsorin, paclitaxel. doxorubicin) was evaluated with Zot (0, 2, and 4 microg/mL) using Caco-2 cell monolayers.


RESULTS
Zot was found to be stable over a 10-day period. Significantly higher (p < 0.05) permeability of the molecular weight markers, in lin, and PEG4000 were observed with Zot (4 microg/mL). The transport of each therapeutic marker was significantly increased with paclitaxel displaying a >3-fold enhancement in Papp values with Zot (4 microg/mL). A 30% decrease in transepithelial electrical resistance values wa observed, which returned to baseline 30 min after Zot was removed.


CONCLUSIONS
Considering the problems of poor oral bioavailability, it is concluded that Zot is a promising drug delivery technology to be used to enhance drug transport across the intestinal mucosa. Future applications are targeted at assessing its usefulness in oral drug delivery using in vivo systems.
Cox
Donna S
DS
Pharmacokinetics Biopharmaceutics Laboratory, Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, 100 Penn Street, AHB, Baltimore, Maryland 21201-6808, USA.
Raje
Sangeeta
S
Gao
Huanling
H
Salama
Noha N
NN
Eddington
Natalie D
ND
D001682:Biological Availability; D018938:Caco-2 Cells / Q000378:metabolism*; D002772:Cholera Toxin / Q000493:pharmacokinetics* / Q000494:pharmacology*; D004731:Endotoxins; D006801:Humans; D008970:Molecular Weight; D010539:Permeability / Q000187:drug effects; D004364:Pharmaceutical Preparations / Q000378:metabolism*
D004731:Endotoxins; D004364:Pharmaceutical Preparations; C077728:zonula occludens toxin, Vibrio cholerae; D002772:Cholera Toxin