The effect of postpartum rumen undegradable protein supplementation on hepatic gluconeogenic enzyme activities in dairy cows with fatty liver.
The effect of postpartum supplementation with rumen undegradable protein on the activities of gluconeogenic enzymes was studied in cows with induced fatty liver. Prepartum liver and blood samples were collected at about one week before the expected date of calving and postpartum samples were collected at 10 and 20 days (d) postpartum. At 10 d postpartum, concentrations of serum nonesterified fatty acids and hepatic triacylglycerol levels were higher than at one wk before parturition. The postpartum increases in nonesterified fatty acids and hepatic triacylglycerols were significantly higher in the cows that were fed extra protein than in the control cows. There were no differences between the groups with regard to postpartum changes in the concentrations of plasma glucose, liver glycogen, and serum insulin. The postpartum increase in the activity of fructose 1-6-bisphosphatase was higher in the test group than in the control group, but the increase in the activity of glucose-6-phosphatase was lower. There were no group differences in the postpartum activities of phosphoenolpyruvate carboxykinase, pyruvate carboxylase, and propionyl-CoA carboxylase. Our results suggest that intense lipolysis released more glycerol in the protein-supplemented cows, which stimulated the activity of fructose 1-6-bisphosphatase. However, postpartum rumen undegradable protein supplementation did not affect the activities of the other enzymes of gluconeogenesis, and fatty liver was even exacerbated.
Murondoti
Absolom
A
Department of Farm Animal Health, Department of Clinical Veterinary Studies, Faculty of Veterinary Science, University of Zimbabwe, Mount Pleasant, Harare, Zimbabwe.
Tivapasi
Musavengana T
MT
Geelen
Math J H
MJ
Wensing
Theo
T
Beynen
Anton C
AC
D000821:Animal Feed; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002417:Cattle; D004044:Dietary Proteins / Q000008:administration & dosage* / Q000378:metabolism*; D019587:Dietary Supplements; D004798:Enzymes / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005234:Fatty Liver / Q000201:enzymology*; D005260:Female; D005943:Gluconeogenesis / Q000502:physiology*; D007328:Insulin / Q000097:blood; D008099:Liver / Q000201:enzymology*; D008112:Liver Glycogen / Q000032:analysis; D049590:Postpartum Period / Q000378:metabolism*; D012417:Rumen / Q000378:metabolism*; D014280:Triglycerides / Q000032:analysis
D001786:Blood Glucose; D004044:Dietary Proteins; D004798:Enzymes; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D008112:Liver Glycogen; D014280:Triglycerides