Differential expression of human 5S snoRNA genes.
Four novel small nucleolar RNAs (snoRNAs), h5sn1, h5sn2, h5sn3, and h5sn4, were successfully amplified from human total RNAs using RT-PCR. They exhibited the structural hallmarks of box H/ACA snoRNAs and formed sequence complementarity to 5S rRNA. The nucleotide sequences of the snoRNAs from different donors were highly conserved as evidenced by single-stranded conformational polymorphism and direct nucleotide sequence analysis. Although their host genes had no protein-coding potential, the expression of the snoRNAs was differentially displayed in different tissues. Noticeably, h5sn2 was highly expressed in normal brain, but its expression drastically decreased in meningioma. This opens the fascinating possibility of the relationship between the processing of snoRNAs and carcinogenesis.
Chang
Long Sen
LS
Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan. lschang@mail.nsysu.edu.tw
Lin
Shel Yio
SY
Lieu
Ann Shung
AS
Wu
Tony Lin
TL
D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D017124:Conserved Sequence; D005796:Genes; D006801:Humans; D008577:Meningeal Neoplasms / Q000235:genetics / Q000378:metabolism; D008579:Meningioma / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D020537:RNA, Small Nucleolar / Q000096:biosynthesis* / Q000235:genetics; D016415:Sequence Alignment; D014018:Tissue Distribution; D014158:Transcription, Genetic
D012341:RNA, Ribosomal, 5S; D020537:RNA, Small Nucleolar