Reprogramming of the activity of the activator/dissociation transposon family during plant regeneration in rice.
Many aspects of epigenetic phenomena have been elucidated via studies of transposable elements. An active transposable element frequently loses its ability to mobilize and goes into an inactive state during development. In this study, we describe the cyclic activity of a maize transposable element dissociation (Ds) in rice. In rice genome, Ds undergoes the spontaneous loss of mobility. However, an inactive state of Ds can be changed into an active state during tissue culture. The recovery of mobility accompanies not only changes in the methylation patterns of the terminal region of Ds, but also alteration in the steady state level of the activator (Ac) mRNA that is expressed by a constitutive CaMV 35S promoter. Furthermore, the Ds-reactivation process is not random, but stage-specific during plantlet regeneration. Our findings have expanded previous observations on Ac reactivation in the tissue culture of maize.
Ki
Chul Min
CM
Division of Applied Life Science, Gyeongsang National University, Jinju, Korea.
Je
Byoung Il
BI
Piao
Hai Long
HL
Par
Soon Ju
SJ
Kim
Min Jung
MJ
Park
Sung Han
SH
Park
Jin Young
JY
Park
Su Hyun
SH
Lee
Eun Kyeong
EK
Chon
Nam Soo
NS
Won
Yong Jae
YJ
Lee
Gi Hwan
GH
Nam
Min Hee
MH
Yun
Doh Won
DW
Lee
Myung Chul
MC
Cha
Young Soon
YS
Le Kon
Ho
H
Eun
Moo Young
MY
Han
Chang-Deok
CD
D004251:DNA Transposable Elements / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D012275:Oryza / Q000235:genetics* / Q000254:growth & development*; D003313:Zea mays / Q000235:genetics
D004251:DNA Transposable Elements