title: T-DNA integration into the Arabidopsis genome depends on sequences of pre-insertion sites.
abstract: A statistical analysis of 9000 flanking sequence tags characterizing transferred DNA (T-DNA) transformants in Arabidopsis sheds new light on T-DNA insertion by illegitimate recombination. T-DNA integration is favoured in plant DNA regions with an A-T-rich content. The formation of a short DNA duplex between the host DNA and the left end of the T-DNA sets the frame for the recombination. The sequence immediately downstream of the plant A-T-rich region is the master element for setting up the DNA duplex, and deletions into the left end of the integrated T-DNA depend on the location of a complementary sequence on the T-DNA. Recombination at the right end of the T-DNA with the host DNA involves another DNA duplex, 2-3 base pairs long, that preferentially includes a G close to the right end of the T-DNA.
authors.affiliation: URGV, UMR en Génomique Végétale, INRA/CNRS/Université Evry-Val d'Essonne, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Brunaud
authors.forename: Sandrine
authors.initials: S
authors.lastname: Balzergue
authors.forename: Bertrand
authors.initials: B
authors.lastname: Dubreucq
authors.forename: Sébastien
authors.initials: S
authors.lastname: Aubourg
authors.forename: Franck
authors.initials: F
authors.lastname: Samson
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Chauvin
authors.forename: Nicole
authors.initials: N
authors.lastname: Bechtold
authors.forename: Corinne
authors.initials: C
authors.lastname: Cruaud
authors.forename: Richard
authors.initials: R
authors.lastname: DeRose
authors.forename: Georges
authors.initials: G
authors.lastname: Pelletier
authors.forename: Loïc
authors.initials: L
authors.lastname: Lepiniec
authors.forename: Michel
authors.initials: M
authors.lastname: Caboche
authors.forename: Alain
authors.initials: A
authors.lastname: Lecharny
mesh_terms: D024509:3' Flanking Region; D024506:5' Flanking Region; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D018014:Gene Transfer Techniques; D018745:Genome, Plant; D030821:Plants, Genetically Modified; D017385:Sequence Homology
chemical_list: D004247:DNA