title: In vitro modulation of C1q mRNA expression and secretion by interleukin-1, interleukin-6, and interferon-gamma in resident and stimulated murine peritoneal macrophages.
abstract: The complement system plays an important role in the humoral immune response. Activation of the classical complement pathway is mediated by its subcomponent, C1q. Among the main C1q-synthesising tissues, macrophages have been attributed as a source of particular importance. We investigated the effects of cytokines (IL-1, IL-6 and Interferon-gamma) on local C1q mRNA expression and C1q secretion in resident and in thioglycollate-stimulated murine peritoneal macrophages in vitro. The macrophages were isolated from murine peritoneal lavage fluid, maintained in culture and incubated with the cytokines. Among the cytokines, only IL-6 had a stimulatory effect on C1q production (25% increase vs. control), while IL-1 and interferon-gamma had an inhibitory effect (50% decrease vs. control), especially in stimulated peritoneal macrophages in culture. Our data suggest that C1q production in macrophages may be differentially regulated by inflammatory cytokines such as IL-1, IL-6 and interferon-gamma, the response being dependent on macrophage activation.
authors.affiliation: Institute of Medical Microbiology and Hygiene, Johannes Gutenberg-University, Mainz, Germany.
authors.forename: Dominik
authors.initials: D
authors.lastname: Faust
authors.forename: Michael
authors.initials: M
authors.lastname: Loos
mesh_terms: D000818:Animals; D015922:Complement C1q / Q000096:biosynthesis* / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins; D013864:Thioglycolates / Q000494:pharmacology
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D011994:Recombinant Proteins; D013864:Thioglycolates; D015922:Complement C1q; D007371:Interferon-gamma