[Analysis of genetic instabilities on chromosome loci 3, 6, 11, and 18 in human cervical carcinoma].
OBJECTIVE
The infection with human papillomavirus and cellular genetic alteration appear to be necessary etiological factors for cervical carcinoma. This study was designed to investigate the genetic instability in cervical carcinoma tissues and provide evidence for discovering new tumor suppressor genes and screening diagnostic molecular marker of cervical carcinoma.


METHODS
Fifty primary cervical carcinoma specimens from high-incidence area was examined through loss of heterozygosity(LOH) and microsatellite instability(MI) analysis with eight polymorphic microsatellite markers on chromosome 3, 6, 11, and 18.


RESULTS
LOH was observed at one or more loci in 33 cases(66%). The highest frequency of the allelic loss was found in D18s474 (18q21, 40.5%). LOH was detected on other sites including D3s1478 (3p21.3-21.2, 31.7%), D3s1766 (18q21.32, 15.0%), D6s260 (6p23, 23.3%), D11s925 (11q22-23, 17.9%), D18s35 (18q21.1-21.31, 8.7%), D18s64 (18q21.32, 16.7%), and D18s68 (18q22.1, 27.3%). MI was detected in 4 cases(8%).


CONCLUSIONS
Different percentages LOH on specific chromosomal regions were found and MI was very infrequent in cervical carcinoma tissues. The results of the current study strongly suggest that the putative suppressor gene(s) likely to be located on specific chromosome region 3p or 18q involve in cervical tumorigenesis.
Zhao
Min
M
Department of Molecular Biology, Medical College of Wuhan University, Wuhan 430071, P. R. China. zhaomin28@hotmail.com
Wu
Xin-xing
XX
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology / Q000821:virology; D002880:Chromosomes, Human, Pair 11; D002887:Chromosomes, Human, Pair 18; D002893:Chromosomes, Human, Pair 3; D002896:Chromosomes, Human, Pair 6; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D050725:Papillomavirus E7 Proteins; D030361:Papillomavirus Infections / Q000235:genetics / Q000473:pathology / Q000821:virology; D014412:Tumor Virus Infections / Q000235:genetics / Q000473:pathology / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology
D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16