A polyaromatic caveolin-binding-like motif in the cytoplasmic tail of the type 1 receptor for angiotensin II plays an important role in receptor trafficking and signaling.
The type 1 receptor for angiotensin II (AT(1)) is a member of the G protein-coupled receptor family. The presence of a caveolin-binding-like motif (phiXphiXXXXphiXXphi where phi is an aromatic residue) within the cytoplasmic tail of the AT(1) receptor suggests an implication for caveolae in the functionality of this receptor. We constructed a mutant AT(1) receptor where each of the aromatic residues in the caveolin-binding-like motif were replaced by alanine (AT(1)-YFFY/A). Mutation of this motif considerably reduced the plasma membrane expression of the receptor that accumulated in a perinuclear compartment. The agonist-induced internalization rate of the AT(1)-YFFY/A receptor was also significantly reduced. Finally, the AT(1)-YFFY/A receptor was poorly activated as indicated by a low agonist-induced production of inositol phosphates. Unexpectedly, the proportion of AT(1) receptor found in caveolae was minor under basal conditions and did not increase under stimulated conditions. Coexpression of the AT(1) receptor with dopamine receptor interacting protein of 78 kDa, a protein implicated in the cellular routing of the dopamine D1 receptor, increased plasma membrane expression of the AT(1) receptor. However, dopamine receptor interacting protein of 78 kDa had no effect on the expression of the AT(1)-YFFY/A receptor. Taken together, these results suggest that the caveolin-binding-like motif of the AT(1) receptor does not promote localization of the receptor to caveolae but rather may act as a docking site for regulatory proteins modulating the routing and the functionality of the receptor.
Leclerc
Patrice C
PC
Department of Pharmacology, Faculty of Medicine, Université de Sherbrooke, Sherbrooke, Québec, Canada J1H 5N4.
Auger-Messier
Mannix
M
Lanctot
Pascal M
PM
Escher
Emanuel
E
Leduc
Richard
R
Guillemette
Gaétan
G
D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D051242:Caveolin 1; D022461:Caveolins / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D005326:Fetal Proteins; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D007457:Iodine Radioisotopes; D007700:Kinetics; D008164:Luminescent Proteins; D008565:Membrane Proteins / Q000494:pharmacology; D018613:Microscopy, Confocal; D018832:Molecular Chaperones; D016296:Mutagenesis; D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D017447:Receptors, Dopamine D1 / Q000235:genetics; D011993:Recombinant Fusion Proteins; D015398:Signal Transduction; D013329:Structure-Activity Relationship; D014162:Transfection
C495540:CAV1 protein, human; D051242:Caveolin 1; D022461:Caveolins; C476535:DNAJC14 protein, human; D005326:Fetal Proteins; D007295:Inositol Phosphates; D007457:Iodine Radioisotopes; D008164:Luminescent Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin; D017447:Receptors, Dopamine D1; D011993:Recombinant Fusion Proteins; D000804:Angiotensin II; D049452:Green Fluorescent Proteins