title: A lipase-inhibiting protein from lipoxygenase-deficient soybean seeds.
abstract: A lipase-inhibiting protein was isolated from lipoxygenase (LOX)-deficient soybean seeds. The molecular mass of the protein was 56.0-kDa and the N-terminal amino acid was blocked. The protein was identified by peptide mass fingerprinting in combination with matrix-assisted laser desorption ionization/time-of-flight mass spectrometry. The masses of the lysyl endopeptidase-digested peptides of the 56.0-kDa inhibiting protein were almost identical to the calculated masses of the theoretically predicted lysyl endopeptidase-treated peptides of beta-amylase from soybean seed. In a previous paper (Biosci. Biotechnol. Biochem., 62, 1498-1503, 1998), we reported that LOX-1, an isozyme of soybean seed LOX, inhibited hydrolysis of soybean oil by pancreatic lipase. Purified beta-amylase also inhibited lipase activity, although the magnitude of inhibition was weaker than that by LOX-1. Thus, there are at least two lipase-inhibiting proteins, one is a LOX and the other is a beta-amylase, in soybean seed.
authors.affiliation: Department of Applied Biological Science, Fukuyama University, Fukuyama 729-0292, Japan. satouchi@fubac.fukuyama-u.ac.jp
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Satouchi
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Kodama
authors.forename: Kaoru
authors.initials: K
authors.lastname: Murakami
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Tanaka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Iwamoto
authors.forename: Masao
authors.initials: M
authors.lastname: Ishimoto
mesh_terms: D000595:Amino Acid Sequence; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008049:Lipase / Q000037:antagonists & inhibitors*; D008084:Lipoxygenase / Q000172:deficiency*; D008969:Molecular Sequence Data; D012639:Seeds / Q000187:drug effects / Q000201:enzymology; D013025:Glycine max / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D001614:beta-Amylase / Q000032:analysis / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D008084:Lipoxygenase; D008049:Lipase; D001614:beta-Amylase