title: Cardiac outflow of amino acids and purines during myocardial ischemia and reperfusion.
abstract: A novel application of microdialysis was studied, in which myocardial outflow of amino acids and purines was monitored by intravasal microdialysis in the myocardial venous outflow during ischemia and reperfusion. Microdialysis catheters were introduced into the great cardiac vein, pulmonary artery, and external jugular vein in 20 anesthetized pigs. The left anterior descending artery was occluded in four groups of pigs for 0, 10, 15, and 60 min. Ischemia was followed by 120 min of reperfusion. Microdialysis samples were analyzed for taurine, aspartate, glutamate, hypoxanthine, inosine, and guanosine. Myocardial infarction developed when ischemia exceeded 10 min. Taurine, aspartate, inosine, and guanosine increased early in the great cardiac vein during ischemia. We found the outflow patterns of amino acids and purines to be graded in response to different lengths of ischemia. In this study we have demonstrated a graded outflow of amino acids and purines in response to ischemia and a positive correlation between infarct size and myocardial outflow of amino acids and purines. This could be of value in a clinical setting to quantify the extent of myocardial damage.
authors.affiliation: Department of Thoracic Surgery, Karolinska Hospital, Karolinska Institutet, S-171 76 Stockholm, Sweden. tobias.backstrom@kirurgi.ki.se
authors.forename: Tobias
authors.initials: T
authors.lastname: Bäckström
authors.forename: Michel
authors.initials: M
authors.lastname: Goiny
authors.forename: Ulf
authors.initials: U
authors.lastname: Lockowandt
authors.forename: Jan
authors.initials: J
authors.lastname: Liska
authors.forename: Anders
authors.initials: A
authors.lastname: Franco-Cereceda
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D005260:Female; D006151:Guanosine / Q000378:metabolism; D006439:Hemodynamics / Q000502:physiology; D019271:Hypoxanthine / Q000378:metabolism; D007288:Inosine / Q000378:metabolism; D008297:Male; D017551:Microdialysis; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000473:pathology; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D011687:Purines / Q000378:metabolism*; D013552:Swine; D013654:Taurine / Q000378:metabolism
chemical_list: D000596:Amino Acids; D011687:Purines; D006151:Guanosine; D013654:Taurine; D019271:Hypoxanthine; D001224:Aspartic Acid; D007288:Inosine