Influence of Angelicae sinensis extract on lipid accumulation in rabbit aortic smooth muscle cells induced by oxidized LDL.
OBJECTIVE
To further establish a definite basic of the application value of sodium ferulate (SF) for prevention and cure of acute coronary heart disease, we examined the effects of SF on LDL oxidation and lipid accumulation in rabbit aortic smooth muscle cells induced by modified-LDL.


METHODS
LDL oxidation was carried out in the presence and absence of SF. Cultured rabbit aortic smooth muscle cells were used as the model. To investigate the effects of SF on intracellular lipid accumulation, cells were incubated with Ox-LDL and SF. The lipid content (cholesterol and triglycerides) of the cells were determined.


RESULTS
Intracellular cholesterol and triglycerides were significantly increased in cell-modified LDL group. The enhancements of above indexes were decreased after addition of SF (200 microg/ml). On the other hand, incubation of LDL with SF resulted in a significant decrease in TBARS activity and electrophoretic mobility.


CONCLUSIONS
The results indicated that SF assume significance both in the protection of LDL against oxidation and inhibition of cell-modified LDL effects on intracellular lipid with the potential to prevent cell foamation.
Traoré
Mariama
M
Mécanique et Ingénierie Cellulaire et Tissulaire, LEMTA, UMR CNRS 7563 Faculté de Médecine, 54500 Vandoeuvre-lès-Nancy, France. Mariama.Traore@hemato.u-nancy.fr
Jialing
Hong
H
Chunling
Xu
X
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D018929:Cell Culture Techniques; D003300:Copper / Q000037:antagonists & inhibitors / Q000494:pharmacology; D003373:Coumaric Acids / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D050356:Lipid Metabolism; D008077:Lipoproteins, LDL / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000494:pharmacology; D011817:Rabbits
D003373:Coumaric Acids; D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; D010936:Plant Extracts; C099252:oxidized low density lipoprotein; D003300:Copper; C004999:ferulic acid