title: Degradation of cholesterol by Bacillus subtilis SFF34 isolated from Korean traditional fermented flatfish.
abstract: OBJECTIVE
  To examine cholesterol degradation by Bacillus subtilis SFF34.
  
  
  RESULTS
  Cholesterol degradation and cholesterol oxidase production by B. subtilis SFF34 were investigated in a medium containing 0.2% cholesterol. In addition, the oxidized product of cholesterol by the purified cholesterol oxidase was detected using a gas chromatograph. Cholesterol oxidase production reached its maximal level (3.14 U ml(-1) after 24 h of incubation in the cholesterol medium. The residual cholesterol content reduced to 0.98 mg g(-1) after 60 h of cultivation in the cholesterol medium. Two cholesterol oxidases were purified from the culture supernatant fluid and their reaction product against cholesterol was identified as 4-cholesten-3-one.
  
  
  CONCLUSIONS
  B. subtilis SFF34 degraded cholesterol and produced a high level of extracellular cholesterol oxidase.
  
  
  CONCLUSIONS
  Bacillus subtilis will be very useful for the reduction of cholesterol in many fermented foods and as a source of cholesterol oxidase.
authors.affiliation: Department of Food Science and Technology, Kyungpook National University, Taegu, Korea.
authors.forename: K P
authors.initials: KP
authors.lastname: Kim
authors.forename: C H
authors.initials: CH
authors.lastname: Rhee
authors.forename: H D
authors.initials: HD
authors.lastname: Park
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism*; D002789:Cholesterol Oxidase / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D005412:Flatfishes / Q000378:metabolism / Q000382:microbiology*; D007723:Korea
chemical_list: D003470:Culture Media; D002784:Cholesterol; D002789:Cholesterol Oxidase