Development of DNA chip for the simultaneous detection of various beta-lactam antibiotic-resistant genes.
A robust and fast DNA chip method was developed in order to detect the various beta-lactam antibiotic-resistance genes in one slide. These genes included PSE, OXA, FOX, MEN, CMY, TEM, SHV, OXY, and AmpC. beta-lactam antibiotic-resistance genes were labeled with a fluorescent nucleotide by a multiplex polymerase chain reaction using a mixture of specific primer sets for each gene. This labeled target was hybridized with a DNA chip that contained the spots of the specific probe DNAs for each beta-lactam antibiotic-resistance gene. This technique made it possible to detect the specific resistance gene, even in a single bacterium.
Lee
Yeonhee
Y
Department of Biotechnology, Seoul Women's University, Korea.
Lee
Choong-Sik
CS
Kim
Yeo-Jung
YJ
Chun
Sungmin
S
Park
Seohyung
S
Kim
Young Sun
YS
Han
Byoung-Don
BD
D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D018440:beta-Lactam Resistance / Q000235:genetics*