[Isolation and identification of cDNA sequence differentially expressed in human lung carcinoma].
OBJECTIVE
The molecular mechanism of the pathogenesis of lung carcinoma is unclear; because key genes related to lung carcinoma have not been identified. This study was designed to isolate and identify differentially expressed cDNA sequences in human lung carcinoma for cloning lung carcinoma-related genes.


METHODS
Using mRNA differential display method combined with cloning and reserve Northern dot blot and sequencing and RT-PCR, differentially expressed cDNA sequences were isolated between two lung cancer cell lines, two adult lung carcinoma tissue and paired normal tissue of trachea epithelia.


RESULTS
Sixteen differentially expressed cDNA fragments were isolated, subsequent cloning of sixteen differentially expressed cDNA fragments were confirmed by reverse Northern dot blot and sequencing and BLAST analysis. Two of these were shown to be novel gene sequences that had not been reported. Eight of the remaining cDNA sequences homologize to known genes; additionally two differentially expressed cDNA sequences were confirmed by RT-PCR.


CONCLUSIONS
Using mRNA differentially display, The authors had successfully isolated ten differentially expressed cDNA sequences from human lung carcinoma, these cDNA sequences might be involved in the pathogenesis of lung carcinoma.
Xie
Hai-long
HL
Laboratory of Tumor Cell Biology, Cancer Research Institute, Xiangya Medical School, Central South University, Changsha 410078, P. R. China.
Li
You-jun
YJ
Chen
Zhu-chu
ZC
Guan
Yong-jun
YJ
He
Chun-mei
CM
D015152:Blotting, Northern; D018076:DNA, Complementary / Q000096:biosynthesis / Q000302:isolation & purification*; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary