In vitro development of bovine embryos encapsulated in sodium alginate.
The objective of this study was to evaluate the in vitro development of bovine embryos encapsulated in alginate. Day-4 embryos produced in vitro (n = 110) were encapsulated with 1.5% sodium alginate and co-cultured with oviduct cells. Unencapsulated embryos (n = 106) were used as controls. In vitro development rate to the blastocyst stage at Day 7 was similar between encapsulated, 42.7%, (47/110) and control. 34% (36/106). embryos. Although encapsulated embryos were able to hatch on Day 9, they did so in a lower proportion than controls (P < 0.05). In conclusion, alginate encapsulation of bovine embryos does not disturb the in vitro development up to the blastocyst stage but significantly reduces the hatching process.
Yániz
J L
JL
Department of Animal Production, Polytechnic School of Huesca, University of Zaragoza, Spain.
Santolaria
P
P
López-Gatius
F
F
D000464:Alginates / Q000008:administration & dosage*; D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D002417:Cattle / Q000196:embryology*; D002478:Cells, Cultured; D003470:Culture Media; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008846:Micromanipulation / Q000379:methods / Q000662:veterinary
D000464:Alginates; D003470:Culture Media; D006603:Hexuronic Acids; D020723:Glucuronic Acid