Characterization of two new members of the formyl peptide receptor gene family from 129S6 mice.
Formyl peptide receptors are G-protein-coupled, seven-transmembrane domain receptors originally identified in leukocytes. Ligands for this class of receptors include the chemotactic peptide fMet-Leu-Phe, lipoxin A(4), serum amyloid A and beta-amyloid peptides. The formyl peptide receptor gene family contains three members in human and six members in mouse. By screening a mouse genomic library, we isolated two novel genes that were provisionally named Fpr-rs6 and Fpr-rs7. They encode putative seven-transmembrane domain proteins of 339 and 338 residues, respectively, and share between them 94% amino acid identity. The predicted amino acid sequences of Fpr-rs6 and Fpr-rs7 are 53-74% identical to other mouse formyl peptide receptors. The transcript of Fpr-rs6 is found in brain, spleen and skeletal muscle, and at high level in testis. The Fpr-rs7 transcript is more widely expressed in heart, liver, lung, spleen, smooth muscle and pancreas. Our data suggest that the expression of Fpr-rs6 and Fpr-rs7 is differentially regulated in mouse despite their high sequence homology.
Wang
Zhen-Guo
ZG
Department of Pharmacology, College of Medicine, University of Illinois, Chicago, IL 60612, USA.
Ye
Richard D
RD
D000595:Amino Acid Sequence; D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000235:genetics*; D018000:Receptors, Peptide / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
C471237:Fpr-rs6 protein, mouse; C471238:Fpr-rs7 protein, mouse; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; D012313:RNA; D004247:DNA