title: Induction of monocyte chemoattractant protein-1 by Porphyromonas gingivalis in human endothelial cells.
abstract: The association between periodontal and cardiovascular diseases could be mediated by direct interaction of periodontal pathogens with cardiac tissue. In order to explore this possibility, the effect of the periodontal pathogen Porphyromonas gingivalis on monocyte chemoattractant protein-1 (MCP-1) production by endothelial cells was investigated. When incubated with live P. gingivalis 381, MCP-1 production by human umbilical vein endothelial cells (HUVEC) was potently increased. Compared to the type strain 381, non-adhesive/invasive strains (W50 and DPG3) did not increase MCP-1 production, which was also demonstrated at the mRNA level. Killed P. gingivalis 381 was much less effective than live bacteria for MCP-1 induction. Treatment of HUVEC with cytochalasin D, an inhibitor of endocytosis, prevented MCP-1 mRNA up-regulation by P. gingivalis 381, suggesting that internalization of P. gingivalis is necessary for MCP-1 induction. In conclusion, the secretion of high levels of MCP-1 resulting from interactions of P. gingivalis with endothelial cells could enhance atherosclerosis progression by contributing to the recruitment of monocytes.
authors.affiliation: Department of Oral Biology, State University of New York, 3435 Main Street, Buffalo, NY 14214, USA.
authors.forename: In-Chol
authors.initials: IC
authors.lastname: Kang
authors.forename: Howard K
authors.initials: HK
authors.lastname: Kuramitsu
mesh_terms: D001161:Arteriosclerosis / Q000503:physiopathology; D001422:Bacterial Adhesion; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D017077:Culture Media, Conditioned; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D016966:Porphyromonas gingivalis / Q000276:immunology* / Q000472:pathogenicity*; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D018932:Chemokine CCL2; D017077:Culture Media, Conditioned; D012333:RNA, Messenger