title: Genotyping analyses of tuberculosis cases in U.S.- and foreign-born Massachusetts residents.
abstract: We used molecular genotyping to further understand the epidemiology and transmission patterns of tuberculosis (TB) in Massachusetts. The study population included 983 TB patients whose cases were verified by the Massachusetts Department of Public Health between July 1, 1996, and December 31, 2000, and for whom genotyping results and information on country of origin were available. Two hundred seventy-two (28%) of TB patients were in genetic clusters, and isolates from U.S-born were twice as likely to cluster as those of foreign-born (odds ratio [OR] 2.29, 95% confidence interval [CI] 1.69 to 3.12). Our results suggest that restriction fragment length polymorphism analysis has limited capacity to differentiate TB strains when the isolate contains six or fewer copies of IS6110, even with spoligotyping. Clusters of TB patients with more than six copies of IS6110 were more likely to have epidemiologic connections than were clusters of TB patients with isolates with few copies of IS6110 (OR 8.01, 95%; CI 3.45 to 18.93).
authors.affiliation: Massahusetts Department of Public Health, Boston, Massahusetts 02130, USA. Sharon.Sharnprapai@state.ma.us
authors.forename: Sharon
authors.initials: S
authors.lastname: Sharnprapai
authors.forename: Ann C
authors.initials: AC
authors.lastname: Miller
authors.forename: Robert
authors.initials: R
authors.lastname: Suruki
authors.forename: Edward
authors.initials: E
authors.lastname: Corkren
authors.forename: Sue
authors.initials: S
authors.lastname: Etkind
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Driscoll
authors.forename: Michael
authors.initials: M
authors.lastname: McGarry
authors.forename: Edward
authors.initials: E
authors.lastname: Nardell
mesh_terms: D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis; D004641:Emigration and Immigration; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008404:Massachusetts / Q000453:epidemiology; D008875:Middle Aged; D017720:Molecular Epidemiology; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors; D014376:Tuberculosis / Q000453:epidemiology* / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial