title: Identification of a fibronectin-binding protein from Staphylococcus epidermidis.
abstract: Staphylococcus epidermidis has been reported to bind to a number of host cell extracellular matrix proteins, including fibronectin. Here we report the identification of a fibronectin-binding protein from S. epidermidis. A phage display library of S. epidermidis genomic DNA was constructed and panned against immobilized fibronectin. A number of phagemid clones containing overlapping inserts were identified, and one of these clones, pSE109FN, contained a 1.4-kb insert. Phage pSE109FN was found to bind to fibronectin but not to collagen, fibrinogen, laminin, or vitronectin. However, pSE109FN also bound to heparin, hyaluronate, and plasminogen, although to a lesser extent than it bound to fibronectin. Analysis of The Institute for Genomic Research S. epidermidis genome sequence database revealed a 1.85-kb region within a putative 30.5-kb open reading frame, to which the overlapping DNA inserts contained within the fibronectin-binding phagemids mapped. We have designated the gene encoding the fibronectin-binding domain embp. A recombinant protein, Embp32, which encompassed the fibronectin-binding domain of Embp, blocked the binding of S. epidermidis, but not the binding of Staphylococcus aureus, to fibronectin. In contrast, a recombinant protein, FnBPB[D1-D4], spanning the fibronectin-binding domain of the S. aureus fibronectin-binding protein FnBPB, blocked binding of S. aureus to fibronectin but had a negligible effect on the binding of S. epidermidis.
authors.affiliation: Cellular Microbiology Research Group, Eastman Dental Institute for Oral Health Care Sciences, University College London, United Kingdom.
authors.forename: Rachel J
authors.initials: RJ
authors.lastname: Williams
authors.forename: Brian
authors.initials: B
authors.lastname: Henderson
authors.forename: Lindsay J
authors.initials: LJ
authors.lastname: Sharp
authors.forename: Sean P
authors.initials: SP
authors.lastname: Nair
mesh_terms: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D003090:Coliphages / Q000235:genetics / Q000378:metabolism; D020650:Combinatorial Chemistry Techniques; D005353:Fibronectins / Q000378:metabolism; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D013212:Staphylococcus epidermidis / Q000235:genetics / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; C469919:Embp32 protein, Staphylococcus epidermidis; D005353:Fibronectins; C078635:fibronectin-binding proteins, bacterial