Chromomycin A3 inhibits influenza a virus multiplication in chick embryo fibroblast cells.
The multiplication of Ulster 73 virus, an avian strain of type A influenza virus, was blocked in chick embryo fibroblast cells, CEF, by treatment with 0.5 microg/ml of chromomycin A3 whereas in LLC-MK2 cells no inhibition of replication was observed. Virus-induced polypeptide synthesis in chick embryo fibroblast cells was confined to the synthesis of PB2, PB1 and PA subunits of the RNA dependent-RNA polymerase, the nucleoprotein NP, the non-structural protein NS1, the haemagglutinin HA, the non-structural protein NS2; only the membrane M1 polypeptide synthesis was greatly inhibited. Viral unpolyadenylated cRNAs synthesis was studied at a late time of the infection, 8 hours p.i.: chromomycin A3 was able to inhibit the "novo" synthesis of complementary RNA poly(A)- and segment 7 of virion RNA. The mode of action of the drug in chick embryo fibroblast cells is discussed.
Conti
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G
Department of Pathology, Medical School, University of Parma, Italy.
Portincasa
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P
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D014128:Chromomycin A3 / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000187:drug effects* / Q000821:virology; D009980:Influenza A virus / Q000187:drug effects* / Q000502:physiology; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology*; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects*
D019384:Nucleic Acid Synthesis Inhibitors; D012367:RNA, Viral; D014128:Chromomycin A3