title: Decreased expression of membrane IL-5 receptor alpha on human eosinophils: II. IL-5 down-modulates its receptor via a proteinase-mediated process.
abstract: In the accompanying study, we demonstrated that following Ag challenge, membrane (m)IL-5Ralpha expression is attenuated on bronchoalveolar lavage eosinophils, soluble (s)IL-5Ralpha is detectable in BAL fluid in the absence of increased steady state levels of sIL-5Ralpha mRNA, and BAL eosinophils become refractory to IL-5 for ex vivo degranulation. We hypothesized that IL-5 regulates its receptor through proteolytic release of mIL-5Ralpha, which in turn contributes to the presence of sIL-5Ralpha. Purified human peripheral blood eosinophils were incubated with IL-5 under various conditions and in the presence of different pharmacological agents. A dose-dependent decrease in mIL-5Ralpha was accompanied by an increase in sIL-5Ralpha in the supernatant. IL-5 had no ligand-specific effect on mIL-5Ralpha or sIL-5Ralpha mRNA levels. The matrix metalloproteinase-specific inhibitors BB-94 and GM6001 and tissue inhibitor of metalloproteinase-3 partially inhibited IL-5-mediated loss of mIL-5Ralpha, suggesting that sIL-5Ralpha may be produced by proteolytic cleavage of mIL-5Ralpha. IL-5 transiently reduced surface expression of beta-chain, but had no effect on the expression of GM-CSFRalpha. Pretreatment of eosinophils with a dose of IL-5 that down-modulated mIL-5Ralpha rendered these cells unable to degranulate in response to further IL-5 stimulation, but they were fully responsive to GM-CSF. These findings suggest that IL-5-activated eosinophils may lose mIL-5Ralpha and release sIL-5Ralpha in vivo, which may limit IL-5-dependent inflammatory events in diseases such as asthma.
authors.affiliation: Allergy and Immunology, Department of Medicine, University of Wisconsin, Madison 53792, USA.
authors.forename: Lin Ying
authors.initials: LY
authors.lastname: Liu
authors.forename: Julie B
authors.initials: JB
authors.lastname: Sedgwick
authors.forename: Mary Ellen
authors.initials: ME
authors.lastname: Bates
authors.forename: Rose F
authors.initials: RF
authors.lastname: Vrtis
authors.forename: James E
authors.initials: JE
authors.lastname: Gern
authors.forename: Hirohita
authors.initials: H
authors.lastname: Kita
authors.forename: Nizar N
authors.initials: NN
authors.lastname: Jarjour
authors.forename: William W
authors.initials: WW
authors.lastname: Busse
authors.forename: Elizabeth A B
authors.initials: EA
authors.lastname: Kelly
mesh_terms: D015550:Cell Degranulation / Q000187:drug effects; D002462:Cell Membrane / Q000276:immunology; D015536:Down-Regulation / Q000187:drug effects; D004804:Eosinophils / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D066298:In Vitro Techniques; D015848:Interleukin-5 / Q000378:metabolism / Q000494:pharmacology*; D061965:Matrix Metalloproteinase Inhibitors; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018123:Receptors, Interleukin / Q000235:genetics* / Q000378:metabolism*; D053649:Receptors, Interleukin-5; D011994:Recombinant Proteins; D012995:Solubility
chemical_list: D015848:Interleukin-5; D061965:Matrix Metalloproteinase Inhibitors; D011480:Protease Inhibitors; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D053649:Receptors, Interleukin-5; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D020782:Matrix Metalloproteinases