A new method for fish leucocyte counting and partial differentiation by flow cytometry.
A simple and rapid method for analysis of fish blood cells is presented. Carp (Cyprinus carpio) blood was diluted 200 times with Hanks' solution containing 1 microg/ml of DiOC6(3) which is a fluorescent, lipophilic dye. After staining for 10 min, the blood cells were measured by a flow cytometer (FACS). Several blood cell populations were identified by different FL-1 (green fluorescence), FSC (forward scatter), and SSC (side scatter) properties. FL-1 v. SSC or FSC v. SSC dot-plot of stained blood cells displayed five separate cell populations: erythrocytes: a mixture of thrombocytes plus lymphocytes; monocytes; neutrophils; and basophils. The number of each type of blood cell counted by the FACS was in good agreement with those counted microscopically.
Inoue
Takamasa
T
Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Kameino 1866, Fujisawa, Kanagawa, 252-8510 Japan.
Moritomo
Tadaaki
T
Tamura
Yumi
Y
Mamiya
Sachiko
S
Fujino
Hiroshi
H
Nakanishi
Teruyuki
T
D000818:Animals; D001772:Blood Cell Count / Q000379:methods / Q000662:veterinary*; D002347:Carps / Q000097:blood*; D002469:Cell Separation / Q000379:methods / Q000662:veterinary*; D004906:Erythrocyte Count / Q000379:methods / Q000662:veterinary; D005434:Flow Cytometry / Q000379:methods / Q000662:veterinary*; D005456:Fluorescent Dyes; D007958:Leukocyte Count / Q000379:methods / Q000662:veterinary; D007962:Leukocytes; D018655:Lymphocyte Count / Q000379:methods / Q000662:veterinary; D010976:Platelet Count / Q000379:methods / Q000662:veterinary; D013194:Staining and Labeling
D005456:Fluorescent Dyes