title: [Improved sensitive determination method for bromate in bread].
abstract: An effective clean-up procedure was developed to determine trace levels of bromate in bread by high-performance liquid chromatography with post-column flow reactor detection. Bromate was extracted from bread with deionized pure water. After centrifugation, the supernatant was filtered through a paper filter. The filtrate was filtered through a 0.2 micron nylon filter and chloride ion was removed by an IC-SP M Ag cartridge column or On-Guard Ag cartridge column. The eluate was applied to an Oasis MAX anion exchange cartridge column. The column was washed with 20% acetic acid and water. Bromate was then eluted with 0.5% sodium nitrate solution. The eluate was determined by HPLC with post column flow reactor detection. The method had a quantitation limit of 2 ng/g in bread products. Recoveries of bromate from bread ranged from 68 to 72% at a spiked bromate level of 2-10 ng/g.
authors.affiliation: National Institute of Health Sciences: 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kawasaki
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kubota
authors.forename: Chikako
authors.initials: C
authors.lastname: Yomota
authors.forename: Tamio
authors.initials: T
authors.lastname: Maitani
mesh_terms: D001939:Bread / Q000032:analysis*; D001959:Bromates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D008722:Methods
chemical_list: D001959:Bromates