The role of BCG in human immune responses induced by multiple injections of autoclaved Leishmania major as a candidate vaccine against leishmaniasis.
To determine if BCG was required in booster injections for autoclaved Leishmania major (ALM) vaccine, 75 volunteers with no response to leishmanin were injected double-blind and randomly with either ALM+BCG or BCG alone for the first injection and boosted either with ALM+BCG, ALM or BCG alone for the second and third. Addition of BCG to the boosters significantly increased the frequency and the magnitude of leishmanin skin tests (LSTs); however, there was no difference in proliferative and IFN-gamma responses (a month and a year later). Three injections of BCG produced no observable adverse reaction; hence BCG could be used in booster injections to increase the protective potential of this candidate vaccine.
Alimohammadian
Mohammad Hossein
MH
Department of Immunology, Pasteur Institute of Iran, Tehran, Iran.
Khamesipour
Ali
A
Darabi
Haiedeh
H
Firooz
Alireza
A
Malekzadeh
Shirin
S
Bahonar
Ahmad
A
Dowlati
Yahya
Y
Modabber
Farrokh
F
D000293:Adolescent; D000328:Adult; D000818:Animals; D001500:BCG Vaccine / Q000276:immunology*; D004311:Double-Blind Method; D004359:Drug Therapy, Combination; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D018320:Leishmania major / Q000276:immunology*; D007896:Leishmaniasis / Q000517:prevention & control*; D054332:Leishmaniasis Vaccines; D008297:Male; D008875:Middle Aged; D016052:Protozoan Vaccines / Q000008:administration & dosage* / Q000276:immunology; D014611:Vaccination; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000009:adverse effects
D001500:BCG Vaccine; D054332:Leishmaniasis Vaccines; D016052:Protozoan Vaccines; D015164:Vaccines, Inactivated; C416262:autoclaved Leishmania major vaccine; D007371:Interferon-gamma