Tumor-specific shared antigenic peptides recognized by human T cells.
The first tumor-specific shared antigens and the cancer-germline genes that code for these antigens were identified with antitumor cytolytic T lymphocytes obtained from cancer patients. A few HLA class I-restricted antigenic peptides were identified by this 'direct approach'. A large set of additional cancer-germline genes have now been identified by purely genetic approaches or by screening tumor cDNA expression libraries with the serum of cancer patients. As a result, a vast number of sequences are known that can code for tumor-specific shared antigens, but most of the encoded antigenic peptides have not yet been identified. We review here recent 'reverse immunology' approaches for the identification of new antigenic peptides. They are based on in vitro stimulation of naive T cells with dendritic cells that have either been loaded with a cancer-germline protein or that have been transduced with viruses carrying cancer-germline coding sequences. These approaches have led to the identification of many new antigenic peptides presented by class I or class II molecules. We also describe some aspects of the processing and presentation of these antigenic peptides.
Van Der Bruggen
Pierre
P
Ludwig Institute for Cancer Research and Cellular Genetics Unit, Université de Louvain, 74 avenue Hippocrate UCL 74.59, B-1200 Brussels, Belgium. pierre.vanderbruggen@bru.licr.org
Zhang
Yi
Y
Chaux
Pascal
P
Stroobant
Vincent
V
Panichelli
Christophe
C
Schultz
Erwin S
ES
Chapiro
Jacques
J
Van Den Eynde
Benoît J
BJ
Brasseur
Francis
F
Boon
Thierry
T
D000595:Amino Acid Sequence; D017951:Antigen Presentation; D000951:Antigens, Neoplasm / Q000145:classification / Q000235:genetics / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology; D041321:Chromosomes, Human, X / Q000235:genetics; D003001:Cloning, Molecular; D016384:Consensus Sequence; D003713:Dendritic Cells / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D009369:Neoplasms / Q000276:immunology*; D009928:Organ Specificity; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013737:Testis / Q000276:immunology; D014161:Transduction, Genetic; D022223:Vaccines, Subunit
D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D018984:Epitopes, T-Lymphocyte; D006680:HLA Antigens; D010446:Peptide Fragments; D022223:Vaccines, Subunit