The apoptotic effect of somatostatin analogue SMS 201-995 on human lymphocytes.
The antiproliferative effect of a synthetic octapeptide, somatostatin analogue SMS 201-995 (SMS), and its capacity to bind were evaluated on human peripheral blood lymphocytes (PBL) activated by phytohemoagglutinin (PHA). We then addressed our work to investigate if SMS inhibits PHA activation of PBL by a cytostatic rather than a cytotoxic mechanism. Consequently, we studied the cell cycle distribution and the activation of caspase-3, measuring the presence of the cleavage product of poly(ADP-ribose) polymerases (PARP), and we evaluated the presence of apoptotic DNA by using a monoclonal antibody specific for the single-stranded regions of DNA. All our results indicate that SMS induces apoptosis in activated lymphocytes.
Lattuada
D
D
Department of Pharmacology, School of Medicine, University of Milan, Via Vanvitelli 32, 20129, Milan, Italy
Casnici
C
C
Venuto
A
A
Marelli
O
O
D000328:Adult; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects* / Q000276:immunology; D053148:Caspase 3; D020169:Caspases / Q000187:drug effects / Q000276:immunology; D002453:Cell Cycle / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000276:immunology; D004247:DNA / Q000187:drug effects; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology* / Q000627:therapeutic use; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D008875:Middle Aged; D015282:Octreotide / Q000494:pharmacology* / Q000627:therapeutic use; D011506:Proteins / Q000187:drug effects / Q000276:immunology; D013004:Somatostatin / Q000031:analogs & derivatives*
D000893:Anti-Inflammatory Agents; D007166:Immunosuppressive Agents; D011506:Proteins; D013004:Somatostatin; D004247:DNA; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; D015282:Octreotide