Somatostatin, cholecystokinin and neuropeptide Y mRNAs in normal and weaver mouse brain.
The distribution of mRNAs encoding for somatostatin, cholecystokinin and neuropeptide Y was determined by in situ hybridization histochemistry in the weaver (wv/wv) mouse, a model of dopamine deficiency as well as in normal (+/+) controls. Weaver mutants did not show any appreciable departure from the normal pattern of expression for mRNA encoding for neuropeptide Y. In contrast, an 82% increase in mRNA encoding for somatostatin was observed in the reticular thalamic nucleus, whereas increases in the order of 20-87% were observed in different hypothalamic nuclei of the weaver brain. In addition, a 47-103% increase of the hybridization signal encoding for cholecystokinin was observed in the cerebral cortex, hippocampus and thalamus of the weaver brain. It can be assumed that the elevated and region-specific somatostatin and cholecystokinin levels observed in the weaver brain may be due to a secondary or compensatory response under conditions of altered neurotransmitter levels.
Zavitsanou
K
K
Department of Physiology, Faculty of Medicine, University of Patras, Patras, Greece.
Triarhou
L C
LC
Kouvelas
E D
ED
Mitsacos
A
A
Palacios
J M
JM
Mengod
G
G
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002766:Cholecystokinin / Q000032:analysis* / Q000235:genetics; D004195:Disease Models, Animal; D004298:Dopamine / Q000172:deficiency*; D017403:In Situ Hybridization; D051379:Mice; D008818:Mice, Neurologic Mutants; D009478:Neuropeptide Y / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D013004:Somatostatin / Q000032:analysis* / Q000235:genetics; D015854:Up-Regulation
D009478:Neuropeptide Y; D012333:RNA, Messenger; D013004:Somatostatin; D002766:Cholecystokinin; D004298:Dopamine