title: New PCR-RFLP analysis for the mouse Tnfsf6gld gene caused by a point mutation in the Tnfsf6 [tumor necrosis factor (ligand) superfamily, member 6] locus.
abstract: A new polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was developed for genetic typing of the mouse Tnfsf6gld mutation. An artificial restriction site was introduced to the mouse Tnfsf6gld mutation by PCR amplification using a modified primer. The three genotypes of the Tnfsf6 locus (Tnfsf6gld/Tnfsf6gld, Tnfsf6gld/+, and +Tnfsf6-gld/+Tnfsf6-gld) could be distinguished clearly and easily. This PCR-RFLP analysis was found to be useful for the identification of the mouse Tnfsf6gld mutation.
authors.affiliation: Japan SLC, Inc., 3-5-1 Aoihigashi, Hamamatsu, Shizuoka 433-8114, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Masui
authors.forename: Yumie
authors.initials: Y
authors.lastname: Takagi
authors.forename: Tetsu
authors.initials: T
authors.lastname: Nishikawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Yanabe
authors.forename: Masato
authors.initials: M
authors.lastname: Nose
authors.forename: Katsunori
authors.initials: K
authors.lastname: Sato
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D004252:DNA Mutational Analysis / Q000379:methods; D017931:DNA Primers; D005838:Genotype; D051379:Mice; D008809:Mice, Inbred C3H / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D017931:DNA Primers; D014409:Tumor Necrosis Factor-alpha