title: [Construction and identification of cDNA library of E.coli mRNA with poly(A) tracts].
abstract: OBJECTIVE
  To construct and identify the cDNA library of E.coli mRNA with poly(A) tracts.
  
  
  METHODS
  The cDNA library of E.coli was constructed by restriction display-PCR (RD-PCR) technique, followed by sequencing and bioinformatics analyses.
  
  
  RESULTS
  cDNA library of E.coli mRNA with poly(A) tracts was successfully constructed, and 66 gene fragments were sequenced.
  
  
  CONCLUSIONS
  The constructed cDNA library of E.coli mRNA with poly(A) tracts contains a low rate of repetition and is of high quality.
authors.affiliation: Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.
authors.forename: Zi-You
authors.initials: ZY
authors.lastname: Hu
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Ma
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Wu
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Song
authors.forename: Qiu-Ye
authors.initials: QY
authors.lastname: Guo
authors.forename: Wen-Ling
authors.initials: WL
authors.lastname: Zheng
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015723:Gene Library; D005798:Genes, Bacterial; D026723:Polyadenylation; D012329:RNA, Bacterial / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D018076:DNA, Complementary; D012329:RNA, Bacterial; D012333:RNA, Messenger