Design of pyridoxal 5'-phosphate dependent catalytic antibodies.
Modern approaches for developing antibodies with coenzyme-dependent activities are discussed for pyridoxal 5'-phosphate dependent transformation of amino acid as an example. A new type of antigens analogous to enzyme--substrate compounds is suggested for the production of such antibodies. Approaches for the development of pyridoxal antiidiotypic antibody using analogs of coenzyme--substrate compounds and corresponding apoenzyme complexes are reviewed.
Khomutov
R M
RM
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 117984 Russia. khomutov@genome.eimb.relarn.ru
D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry / Q000378:metabolism; D017151:Antibodies, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D000941:Antigens / Q000737:chemistry / Q000378:metabolism; D001051:Apoenzymes / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D015202:Protein Engineering; D011732:Pyridoxal Phosphate / Q000737:chemistry / Q000378:metabolism*
D000888:Antibodies, Anti-Idiotypic; D017151:Antibodies, Catalytic; D000941:Antigens; D001051:Apoenzymes; D011732:Pyridoxal Phosphate