Cryopreservation of Citrus madurensis embryonic axes encapsulation-dehydration.
In this paper, we demonstrate that C. madurensis embryonic axes can withstand cryopreservation using the encapsulation-dehydration technique. Up to 57.5 % survival was achieved using a standard encapsulation-dehydration protocol, which included pregrowth of encapsulated axes for 16 h in medium containing 0.8 M sucrose + 1 M glycerol, desiccation of beads to around 30 % moisture content (fresh weight basis) followed by rapid freezing. A slightly higher survival percentage (65 %) was obtained using a modified encapsulation-dehydration protocol, which included pretreatment of axes with 2 M glycerol + 0.6 M sucrose for 1 h, concomitantly with their encapsulation in 3 % calcium alginate beads, followed by desiccation of the beads to around 30 % moisture content.
Cho
Eun Gi
EG
International Plant Genetic Resources Institute (IPGRI), Regional Office for Asia, Pacific and Oceania, P.O. Box 236, UPM Post Office, 43400 Serdang, Selangor Darul Ehsan, Malaysia. e.cho@rda.go.kr.
Hor
Y L
YL
Kim
Haeng Hoon
HH
Rao
V Ramanatha
VR
Engelmann
Florent
F
D002957:Citrus / Q000196:embryology*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D046508:Culture Techniques / Q000379:methods; D003890:Desiccation / Q000379:methods*; D012639:Seeds / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development*; D015996:Survival Rate; D013997:Time Factors; D014867:Water / Q000032:analysis
D003451:Cryoprotective Agents; D014867:Water