[Mutagen effects in various organs and tissues of rats studied by fluorometric analysis].
The method of fluorometric analysis for DNA unity (FADU) was used to study the genotoxic effects of cyclophosphamide in rats. The dose dependence and time variation of mutagenicity manifestations in various organs and tissues of the experimental animals was studied. It was established that FADU results agree with the data obtained by conventional methods of cytogenetics. The results confirm the expediency of using FADU for the preclinical investigations of drugs.
Usol'tsev
M V
MV
Institute of Pharmacology, Russian Academy of Medical Sciences, Baltiiskaya ul. 8, Moscow, 125315 Russia.
Kapkova
Ia L
IaL
Durnev
A D
AD
Seredenin
S B
SB
D000818:Animals; D003520:Cyclophosphamide / Q000633:toxicity*; D004247:DNA / Q000032:analysis / Q000187:drug effects*; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000032:analysis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D009691:Nucleic Acid Denaturation; D009928:Organ Specificity; D051381:Rats; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D004277:DNA, Single-Stranded; D009153:Mutagens; D003520:Cyclophosphamide; D004247:DNA