title: Crystallization of Pichia pastoris lysyl oxidase.
abstract: A copper-containing amine oxidase (PPLO) from the yeast Pichia pastoris has been purified and crystallized in two forms. PPLO is a glycoprotein. The molecular mass from SDS-polyacrylamide gels is 112 kDa, consistent with 20% glycosylation by weight (the calculated molecular weight of the polypeptide is 89.7 kDa). Orthorhombic crystals belonging to space group P2(1)2(1)2(1), with unit-cell parameters a = 163.7, b = 316.1, c = 84.0 A, diffract to 2.65 A resolution. Monoclinic crystals belonging to space group C2, with unit-cell parameters a = 248.4, b = 121.1, c = 151.8 A, beta = 124.6 degrees, diffract to 1.65 A resolution. Native data have been recorded from each crystal form at 100 K using synchrotron radiation. A self-rotation function for the monoclinic crystal form reveals the presence of a non-crystallographic twofold axis perpendicular to the crystallographic twofold axis, consistent with the presence of two dimers in the asymmetric unit.
authors.affiliation: School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia.
authors.forename: Mihwa
authors.initials: M
authors.lastname: Lee
authors.forename: Katrina
authors.initials: K
authors.lastname: Willingham
authors.forename: David
authors.initials: D
authors.lastname: Langley
authors.forename: Megan J
authors.initials: MJ
authors.lastname: Maher
authors.forename: Aina E
authors.initials: AE
authors.lastname: Cohen
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Ellis
authors.forename: Jason A
authors.initials: JA
authors.lastname: Kuchar
authors.forename: David M
authors.initials: DM
authors.lastname: Dooley
authors.forename: Hans C
authors.initials: HC
authors.lastname: Freeman
authors.forename: J Mitchell
authors.initials: JM
authors.lastname: Guss
mesh_terms: D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D010843:Pichia / Q000201:enzymology*; D011487:Protein Conformation; D008249:Protein-Lysine 6-Oxidase / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D008249:Protein-Lysine 6-Oxidase