title: Molecular characterization of foot-and-mouth disease virus O/SKR/2000.
abstract: Molecular cloning and sequencing of the genome of foot-and-mouth disease virus (FMDV) O/SKR/2000, one of PanAsia strain, were performed from FMDV infected cattle. From the poly (C) tract of the 5' nontranslated region (NTR) to the 3' NTR including 14 base pairs (bp) of poly (A) tail, 7813 bp sequences comprising approximately 95% of the whole genome were obtained by reverse transcription polymerase reaction (RT-PCR). The deduced amino acid sequences of the structural and nonstructural proteins (NSP) of the O/SKR/2000 virus were analyzed for the sequence similarity among type O strains. Comparison between FMDV O/SKR/2000 and other strains indicates that overall the number of amino acids appears to be conserved without any deletion in either NSP or capsid proteins, thus, suggesting that O/SKR/2000 evolved with minor difference from preexisting strains.
authors.affiliation: Virology Research Division, National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, Anyang, South Korea. kweonch@mail.nvrqs.go.kr
authors.forename: Chang Hee
authors.initials: CH
authors.lastname: Kweon
authors.forename: Young Joon
authors.initials: YJ
authors.lastname: Ko
authors.forename: Won Il
authors.initials: WI
authors.lastname: Kim
authors.forename: Byung Joon
authors.initials: BJ
authors.lastname: Kwon
authors.forename: Bang Hun
authors.initials: BH
authors.lastname: Hyun
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: Sohn
authors.forename: Kang Seuk
authors.initials: KS
authors.lastname: Choi
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Shin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D003001:Cloning, Molecular; D005536:Foot-and-Mouth Disease / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins