title: Molecular cloning using the interrupted recognition sequence of BplI to select for recombinant molecules.
authors.affiliation: University of Western Ontario, London, Canada.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Mastrodicasa
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Brandl
mesh_terms: D025065:Borrelia burgdorferi / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics / Q000302:isolation & purification*; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D015723:Gene Library; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D013379:Substrate Specificity; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial; D004274:DNA, Recombinant; D015246:Deoxyribonuclease EcoRI; C109633:endodeoxyribonuclease BplI; D015252:Deoxyribonucleases, Type II Site-Specific