Protein engineering 20 years on.
It is 20 years since site-directed mutagenesis was first used to modify the active site of an enzyme of known structure and mechanism. Since then, this method has contributed far-reaching insights into catalysis, specificity, stability and folding of proteins. Engineered proteins are now being used in industry and for the improved treatment of human disease.
Brannigan
James A
JA
Department of Chemistry, University of York, York YO10 5YW, UK. jab@ysbl.york.ac.uk
Wilkinson
Anthony J
AJ
D000906:Antibodies / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D006454:Hemoglobins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000737:chemistry / Q000235:genetics; D015394:Molecular Structure; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D015202:Protein Engineering; D014448:Tyrosine-tRNA Ligase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D000906:Antibodies; D006454:Hemoglobins; D007328:Insulin; D014448:Tyrosine-tRNA Ligase