Rhodopseudomonas acidophila strain 10050 contains photosynthetic LH2 antenna complexes that are not enriched with phosphatidylglycerol, and the phospholipids have a fatty acyl composition that is unusual for purple non-sulfur bacteria.
The phospholipid composition of Rhodopseudomonas acidophila strain 10050 grown aerobically or anaerobically in the light was determined. The major phospholipids present in the aerobic cells were phosphatidylethanolamine (PE; 54%), phosphatidylglycerol (PG; 24%) and cardiolipin (diphosphatidylglycerol, DPG) (14%), together with phosphatidylcholine (PC; 5%). On moving the cells to anaerobic photosynthetic growth in the light PE remained the major phospholipid (37-49%), but there was a major change in the proportion of PC, which increased to 31-33%, and corresponding reductions in the contents of PG to 11-16% and DPG to 4-5%. The fatty acid composition of the phospholipids was unusual, compared with other purple non-sulfur photosynthetic bacteria, in that it contained 16:0 (29%), 17:1 (20%) and 19:1 (9%) plus several mainly unsaturated 2-OH fatty acids (9% total) as major components, when grown aerobically in the dark. In contrast when grown photosynthetically under anaerobic conditions there was <2% 17:1 or 19:1 present, while the amounts of 16:1 and 18:1 increased, and 16:0 decreased. The phospholipid composition of the purified light-harvesting complex 2 (LH2) complex was PE (43%), PC (42%) and DPG (15%). Unexpectedly, there was no PG associated with the purified LH2. These findings contrast with previous studies on several other photosynthetic bacteria, which had shown an increase in PG upon photosynthetic growth [Biochem. J. 181 (1979) 339]. The prior hypothesis that phosphatidylglycerol has some specific role to play in the function of light-harvesting complexes cannot be true for Rps. acidophila. It is suggested that specific integral membrane proteins may strongly influence the phospholipid content of the host membranes into which they are inserted.
Russell
Nicholas J
NJ
Department of Agricultural Sciences, Imperial College, Wye Campus, Ashford, Kent TN25 5AH, UK. nicholas.russell@ic.ac.uk
Coleman
Julie K
JK
Howard
Tina D
TD
Johnston
Evelyn
E
Cogdell
Richard J
RJ
D001426:Bacterial Proteins / Q000737:chemistry; D002458:Cell Fractionation; D002462:Cell Membrane / Q000737:chemistry; D008027:Light; D046911:Macromolecular Substances; D010715:Phosphatidylglycerols / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry; D020836:Protein Structure, Quaternary; D012241:Rhodopseudomonas / Q000737:chemistry* / Q000378:metabolism
D001426:Bacterial Proteins; D046911:Macromolecular Substances; D010715:Phosphatidylglycerols; D010743:Phospholipids; D045322:Photosynthetic Reaction Center Complex Proteins