Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia.
Micro-RNAs (miR genes) are a large family of highly conserved noncoding genes thought to be involved in temporal and tissue-specific gene regulation. MiRs are transcribed as short hairpin precursors ( approximately 70 nt) and are processed into active 21- to 22-nt RNAs by Dicer, a ribonuclease that recognizes target mRNAs via base-pairing interactions. Here we show that miR15 and miR16 are located at chromosome 13q14, a region deleted in more than half of B cell chronic lymphocytic leukemias (B-CLL). Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority ( approximately 68%) of CLL cases.
Calin
George Adrian
GA
Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Dumitru
Calin Dan
CD
Shimizu
Masayoshi
M
Bichi
Roberta
R
Zupo
Simona
S
Noch
Evan
E
Aldler
Hansjuerg
H
Rattan
Sashi
S
Keating
Michael
M
Rai
Kanti
K
Rassenti
Laura
L
Kipps
Thomas
T
Negrini
Massimo
M
Bullrich
Florencia
F
Croce
Carlo M
CM
D000328:Adult; D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology; D015152:Blotting, Northern; D015153:Blotting, Western; D018956:CD5 Antigens / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D017353:Gene Deletion; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D006822:Hybrid Cells / Q000737:chemistry; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D009369:Neoplasms / Q000235:genetics / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000737:chemistry; D014066:Palatine Tonsil / Q000166:cytology; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000737:chemistry
D018956:CD5 Antigens; D035683:MicroRNAs; D012334:RNA, Neoplasm