title: Yersinia effector YopJ inhibits yeast MAPK signaling pathways by an evolutionarily conserved mechanism.
abstract: Yersinia effector, YopJ, inhibits the innate immune response by blocking MAP kinase and NFkappaB signaling pathways in mammalian cells. Herein, YopJ is shown to disrupt the MAP kinase signaling pathways in Saccharomyces cerevisiae. Expression of YopJ in yeast blocks the ability of yeast to respond to alpha factor by disrupting activation of the pheromone signaling pathway upstream of the activation of the MAPK Fus3p. YopJ also blocks the high osmolarity growth (HOG) MAP kinase pathway in yeast upstream of the activation of the MAPK Hog1p. YopJ is proposed to block the MAP kinase pathways in yeast in a similar manner to the way it blocks mammalian signaling pathways, implicating that a novel, evolutionarily conserved mechanism of regulation is utilized for signal transduction by these pathways.
authors.affiliation: Department of Molecular Biology, University of Texas Southwestern Medical School, Dallas 75390-9148, USA.
authors.forename: Sara
authors.initials: S
authors.lastname: Yoon
authors.forename: Zhengchang
authors.initials: Z
authors.lastname: Liu
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Eyobo
authors.forename: Kim
authors.initials: K
authors.lastname: Orth
mesh_terms: D001426:Bacterial Proteins / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D005822:Genetic Vectors; D020935:MAP Kinase Signaling System; D008954:Models, Biological; D016328:NF-kappa B / Q000378:metabolism; D009997:Osmotic Pressure; D010675:Pheromones / Q000378:metabolism; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors; D015007:Yersinia / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins; D016328:NF-kappa B; D010675:Pheromones; C109227:YopP protein, Yersinia