Autoantibodies from an SLE patient immunostain the Barr body.
To identify specific autoimmune disorders that produce autoantibodies against the mammalian Barr body, sera from 185 autoimmune patients were screened using indirect immunofluorescence on human fibroblasts. Serum from a patient with systemic lupus erythematosus immunostained epi- topes concentrated at the Barr body in female fibroblasts. Such autoantibodies provide a novel tool for characterization of Barr body composition and structure.
Brooks
W H
WH
Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Satoh
M
M
Hong
B
B
Reeves
W H
WH
Yang
T P
TP
D001323:Autoantibodies / Q000097:blood*; D001327:Autoimmune Diseases / Q000276:immunology; D002460:Cell Line; D002869:Chromosome Aberrations; D004303:Dosage Compensation, Genetic; D005260:Female; D005347:Fibroblasts; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D066298:In Vitro Techniques; D008180:Lupus Erythematosus, Systemic / Q000235:genetics / Q000276:immunology*; D008297:Male; D012728:Sex Chromatin / Q000276:immunology*
D001323:Autoantibodies