Protection of peroxide-treated fish erythrocytes by coelenterazine and coelenteramine.
European seabass (Dicentrarchus labrax) erythrocytes treated with tert-butyl hydroperoxide (t-BHP) showed decreasing levels of reduced glutathione, increased lipid peroxidation and DNA damage, and ultimately underwent haemolysis. The addition of the marine luciferin coelenterazine (CLZn) markedly delayed the onset of the haemolytic process induced by t-BHP as well as lipid peroxidation and glutathione oxidation. CLZn also protected the red blood cells' DNA against t-BHP-triggered damage. CLZn's oxidation product coelenteramine (CLM) also delayed the lysis of the cells as well as the occurrence of oxidative stress indicators but it did not offer protection against DNA damage. Both compounds proved more efficient than the vitamin E analogue Trolox C at similar doses. These results demonstrate the ability of CLZn and CLM to protect fish cells against oxidative stress, providing further support to the evolutionary model suggesting that CLZn's first physiological role was that of an antioxidant in fish thriving in surface layers of the ocean, later evolving into its light-emitting function in deep-sea species.
Janssens
Bernadette J
BJ
Animal Biology Unit, Université catholique de Louvain, Croix du Sud 5, B-1348 Louvain-la-Neuve, Belgium. janssens@bani.ucl.ac.be
Marchand-Brynaert
Jacqueline
J
Rees
Jean-François
JF
D000818:Animals; D001492:Bass / Q000097:blood*; D002839:Chromans / Q000494:pharmacology; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D007093:Imidazoles; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress; D011719:Pyrazines / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
D002839:Chromans; D007093:Imidazoles; D011719:Pyrazines; D017392:Thiobarbituric Acid Reactive Substances; C000198:2-amino-3-benzyl-5-(p-hydroxyphenyl)pyrazine; C017144:coelenterazine; D020122:tert-Butylhydroperoxide; D005978:Glutathione; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid