title: Arginine metabolic pathways involved in the modulation of tumor-induced angiogenesis by macrophages.
abstract: Neovascularization, an essential step for tumor progression and metastasis development, can be modulated by the presence of macrophages (Mps) in the tumor microenvironment. The ability of Mps to regulate the angiogenicity of the LMM3 tumor cell line was studied. Peritoneal Mps from LMM3 tumor-bearing mice (TMps) potentiate in vivo LMM3 angiogenicity. These results were confirmed by CD31 immunoblotting assays. The activity of TMps depended on nitric oxide synthase (NOS) and arginase (A) activity. By immunoblotting we evidenced that AI and AII isoforms were up-regulated in TMps while the inducible and neuronal NOS isoforms were highly expressed in normal Mps. TMps might positively modulate tumor growth by stimulating angiogenic cascade mainly through polyamine synthesis.
authors.affiliation: Instituto de Oncología Angel H. Roffo, Av. San Martín 5481, CP 1417, Buenos Aires, Argentina.
authors.forename: Lilia Elena
authors.initials: LE
authors.lastname: Davel
authors.forename: María Adela
authors.initials: MA
authors.lastname: Jasnis
authors.forename: Eulalia
authors.initials: E
authors.lastname: de la Torre
authors.forename: Tomomi
authors.initials: T
authors.lastname: Gotoh
authors.forename: Miriam
authors.initials: M
authors.lastname: Diament
authors.forename: Gabriela
authors.initials: G
authors.lastname: Magenta
authors.forename: E
authors.initials: E
authors.lastname: Sacerdote de Lustig
authors.forename: María Elena
authors.initials: ME
authors.lastname: Sales
mesh_terms: D000818:Animals; D001119:Arginase / Q000502:physiology; D001120:Arginine / Q000378:metabolism*; D005260:Female; D007527:Isoenzymes / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000201:enzymology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000098:blood supply* / Q000201:enzymology / Q000378:metabolism*; D009389:Neovascularization, Pathologic; D019001:Nitric Oxide Synthase / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: D007527:Isoenzymes; D001120:Arginine; D019001:Nitric Oxide Synthase; D001119:Arginase