Immobilization of oligonucleotides on poly(ethylene glycol) brush-coated Si surfaces.
High-density poly(ethylene glycol) (PEG) molecules are grafted onto Si surfaces in a brush-like configuration. We demonstrate that this surface is an excellent substrate for oligonucleotide immobilization. p-Maleimidophenyl isocyanate is used as a heterobifunctional cross-linker to tether thiol-modified oligonucleotides to terminal OH groups on the PEG brush. This approach gives excellent immobilization specificity and low background. The immobilized oligonucleotides show high sensitivity for the detection of complementary targets.
Cha
T-W
TW
Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
Boiadjiev
V
V
Lozano
J
J
Yang
H
H
Zhu
X-Y
XY
D001483:Base Sequence; D003432:Cross-Linking Reagents / Q000737:chemistry; D003485:Cyanates / Q000737:chemistry; D008301:Maleimides / Q000737:chemistry; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012825:Silicon / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D014966:Xanthenes / Q000737:chemistry
D003432:Cross-Linking Reagents; D003485:Cyanates; D008301:Maleimides; D009841:Oligonucleotides; D013438:Sulfhydryl Compounds; D014966:Xanthenes; C082056:4-maleimidophenyl isocyanate; D011092:Polyethylene Glycols; C034657:Texas red; D012825:Silicon