IgG antibodies to enterobacteria 60 kDa heat shock proteins in the sera of HLA-B27 positive ankylosing spondylitis patients.
OBJECTIVE
To study the association of HLA-B27 and the IgG response to the 60 kDa HSPs of Klebsiella pneumoniae, Yersinia enterocolitica, Shigella flexneri, Escherichia coli and Salmonella typhi.


METHODS
IgG against the 60 kDa HSPs of enterobacteria was determined by ELISA in the sera from 49 HLA-B27+ ankylosing spondylitis (AS) patients; 41 HLA-B27+ healthy relatives of AS patients and 101 HLA-B27-unrelated healthy individuals.


RESULTS
HLA-B27+ patients and healthy individuals, showed significantly higher IgG antibody levels to the Klebsiella, Yersinia and Salmonella HSPs than HLA-B27- healthy controls. B27+ patients had a significantly higher response to E. coli HSP than the two other groups. IgG response anti-Shigella HSP was similar in the three groups.


CONCLUSIONS
There is a relationship between HLA-B27 and the response to HSPs 60 from Klebsiella, Yersinia, Escherichia and Salmonella, that may be important in the initiation of AS.
Dominguez-López
M L
ML
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, SSa, México.
Burgos-Vargas
R
R
Galicia-Serrano
H
H
Bonilla-Sánchez
M T
MT
Rangel-Acosta
H H
HH
Cancino-Diaz
M E
ME
Jiménez-Zamudio
L
L
Granados
J
J
García-Latorre
E
E
D000907:Antibodies, Bacterial / Q000097:blood; D018834:Chaperonin 60 / Q000096:biosynthesis / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004755:Enterobacteriaceae / Q000276:immunology; D004756:Enterobacteriaceae Infections / Q000097:blood / Q000150:complications / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D015796:HLA-B27 Antigen / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D013167:Spondylitis, Ankylosing / Q000097:blood / Q000276:immunology* / Q000382:microbiology
D000907:Antibodies, Bacterial; D018834:Chaperonin 60; D015796:HLA-B27 Antigen; D007074:Immunoglobulin G