title: Specific binding of silkworm Bombyx mori 30-kDa lipoproteins to carbohydrates containing glucose.
abstract: Insect lectins are important as part of nonspecific self-defense, but their antifungal mechanisms remain to be elucidated. Fungi contain glucans on the cell surface and insect glucan-binding proteins are considered to be essential for antifungal mechanisms. We purified glucose-binding proteins from hemolymph of pupae of the silkworm Bombyx mori, and the amino acid sequence analysis showed that their two proteins are 30-kDa lipoproteins, major components of B. mori hemolymph. These lipoproteins specifically bound to glucose and glucans, suggesting that they are involved in insect self-defense systems.
authors.affiliation: Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agriculture, Meijo University, Tempaku-ku, Nagoya 468-8502, Japan. ujita@ccmfs.meijo-u.ac.jp
authors.forename: Minoru
authors.initials: M
authors.lastname: Ujita
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kimura
authors.forename: Daisuke
authors.initials: D
authors.lastname: Nishino
authors.forename: Eiji
authors.initials: E
authors.lastname: Yokoyama
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Banno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fujii
authors.forename: Akira
authors.initials: A
authors.lastname: Hara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D005936:Glucans / Q000737:chemistry; D005947:Glucose / Q000378:metabolism*; D006458:Hemolymph / Q000737:chemistry / Q000378:metabolism; D008074:Lipoproteins / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009004:Monosaccharide Transport Proteins / Q000737:chemistry / Q000302:isolation & purification; D011485:Protein Binding; D011679:Pupa
chemical_list: D005936:Glucans; D008074:Lipoproteins; D009004:Monosaccharide Transport Proteins; D005947:Glucose