Effects of serum-free culture media on in vitro development of domestic cat embryos following in vitro maturation and fertilization.
This study was conducted to determine the adequate medium for a serum-free culture system of domestic cat embryos produced by in vitro maturation (IVM) and fertilization (IVF). Cumulusoocyte complexes recovered from cat ovaries were matured in vitro for 24 h, and then inseminated in vitro for 12 h. After insemination, the oocytes were cultured in five media [Ham's F10, Waymouth 752/1 (Waymouth), TCM199, modified Earle's balanced salt solution (MK-1) and CR1aa], each of which contained 0.4% bovine serum albumin. There were no significant differences among the rates of fertilization of oocytes cultured in five media following IVF. The rate of oocytes/embryos developed to at least the morula stage was significantly lower (p < 0.05) in Waymouth than in MK-1, TCM199 and CR1aa. Moreover, none of the embryos cultured in Ham's F10 and Waymouth developed to the blastocyst stage. There were no differences among the rates of development to the blastocyst stage of oocytes/embryos cultured in MK-1, TCM199 and CR1aa. These results indicate that the type of serum-free medium has a major impact on in vitro development of domestic cat embryos derived from IVM/IVF, and MK-1, TCM199 and CR1aa media are suitable for in vitro culture of cat embryos in a serum-free culture system.
Murakami
M
M
Laboratory of Animal Reproduction and Biotechnology, Department of Veterinary Sciences, Yamaguchi University, Yamaguchi, Japan.
Otoi
T
T
Karja
N W K
NW
Ooka
A
A
Suzuki
T
T
D000818:Animals; D001947:Breeding; D002415:Cats / Q000196:embryology*; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D004622:Embryo, Mammalian / Q000187:drug effects* / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D008297:Male; D011247:Pregnancy; D012661:Semen
D016895:Culture Media, Serum-Free