Identification of 30 protein species involved in replicative senescence and stress-induced premature senescence.
Exposure of human proliferative cells to subcytotoxic stress triggers stress-induced premature senescence (SIPS) which is characterized by many biomarkers of replicative senescence. Proteomic comparison of replicative senescence and stress-induced premature senescence indicates that, at the level of protein expression, stress-induced premature senescence and replicative senescence are different phenotypes sharing however similarities. In this study, we identified 30 proteins showing changes of expression level specific or common to replicative senescence and/or stress-induced premature senescence. These changes affect different cell functions, including energy metabolism, defense systems, maintenance of the redox potential, cell morphology and transduction pathways.
Dierick
Jean François
JF
Unit of Research on Cellular Biology (URBC), Department of Biology, University of Namur (FUNDP), Rue de Bruxelles 61, B-5000, Namur, Belgium.
Kalume
Dário E
DE
Wenders
Frédéric
F
Salmon
Michel
M
Dieu
Marc
M
Raes
Martine
M
Roepstorff
Peter
P
Toussaint
Olivier
O
D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D016922:Cellular Senescence / Q000502:physiology*; D017931:DNA Primers; D015180:Electrophoresis, Gel, Two-Dimensional; D011506:Proteins / Q000737:chemistry* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D011506:Proteins