title: Broad-host-range mobilizable suicide vectors for promoter trapping in gram-negative bacteria.
abstract: Here we report the construction of three different vectors for the identification of bacterial genes induced in vitro and/or in vivo. These plasmids contain kanamycin, gentamicin, or tetracycline resistance genes as selectable markers. A promoterless cat and an improved GFP (mut3-gfp) can be used to follow the induction of gene expression by measuring chloramphenicol resistance and fluorescence, respectively.
authors.affiliation: Université Laval, Ste-Foy, Québec, Canada.
authors.forename: Mounir
authors.initials: M
authors.lastname: Izallalen
authors.forename: Roger C
authors.initials: RC
authors.lastname: Levesque
authors.forename: Xavier
authors.initials: X
authors.lastname: Perret
authors.forename: William J
authors.initials: WJ
authors.lastname: Broughton
authors.forename: Hani
authors.initials: H
authors.lastname: Antoun
mesh_terms: D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D003227:Conjugation, Genetic / Q000235:genetics; D004265:DNA Helicases / Q000235:genetics; D004268:DNA-Binding Proteins; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005111:Extrachromosomal Inheritance; D005470:Fluorometry; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics*; D023281:Genomics; D006090:Gram-Negative Bacteria / Q000235:genetics*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D009586:Nitrogen Fixation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D008164:Luminescent Proteins; D015534:Trans-Activators; C045264:replication initiator protein; D049452:Green Fluorescent Proteins; D015500:Chloramphenicol O-Acetyltransferase; D004265:DNA Helicases