Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the expression of synaptic proteins in dissociated rat cortical cells.
We investigated the effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the expression of synaptic proteins in dissociated E18 rat cortical cells. TCDD (0-50 nM) was added to plating media, and cell viability and expression of synaptic proteins were assayed on 4 and 7 days in vitro (DIV), respectively. TCDD had no apparent effect on early neurite outgrowth 12 h after plating. However, on 4 DIV, cell viability was reduced significantly, and neurons often revealed vacuoles in 20 or 50 nM culture and had limited secondary or higher order dendritic processes in 20 or 50 nM culture. Immunoblot analyses of cell homogenates indicated upregulation of NMDA receptor subunits (NR1, NR2A, and NR2B), but downregulation of synaptic organizing proteins (PSD-95, densin-180, and septin6 homologue) and a synapse-enriched enzyme (alphaCaMKII). Changes in the expression of synaptic proteins may be a underlying mechanism for altered synaptic transmission and neuropathy by TCDD.
Cho
Sun-Jung
SJ
Department of Microbiology, College of Natural Sciences, Kyungpook National University, Daegu, Korea.
Jung
Jae-Seob
JS
Jin
Ingnyol
I
Jung
Yong Wook
YW
Ko
Bok Hyun
BH
Nam
Kyung Soo
KS
Park
In Kook
IK
Moon
Il Soo
IS
D000818:Animals; D002460:Cell Line; D002540:Cerebral Cortex / Q000378:metabolism*; D004785:Environmental Pollutants / Q000494:pharmacology* / Q000633:toxicity; D066298:In Vitro Techniques; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology* / Q000633:toxicity; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000096:biosynthesis / Q000235:genetics; D013569:Synapses / Q000187:drug effects / Q000378:metabolism*
D004785:Environmental Pollutants; C409466:NR1 NMDA receptor; D000072317:Polychlorinated Dibenzodioxins; D016194:Receptors, N-Methyl-D-Aspartate