Platelet alpha- and gamma-synucleins in Parkinson's disease and normal control subjects.
Alpha-synuclein (alphaSN) has been implicated in Parkinson's Disease (PD) and alphaSN is a major component of Lewy bodies (LBs). This study explored platelets as a model system for study of alphaSN metabolism and platelet alphaSN as a diagnostic marker for PD. We used Western blot analysis to characterize and compare platelet and brain alpha-, beta- and gammaSN; and to quantitate alphaSN levels in platelets from PD and age-matched controls. We found that platelets contain full-length alphaSN and 6 and 12 kDa fragments, and gammaSN-like protein. alphaSN and gammaSN were not secreted by thrombin-activated platelets. Furthermore, we also found that the alphaSN and gammaSN levels in sporadic PD patients and age-matched normal controls were not significantly different. This indicates that platelet alphaSN or gammaSN is not a suitable peripheral diagnostic marker for PD. Platelets may be used for study of alphaSN and gammaSN metabolism, and may give some broad insight into the normal functions of alphaSN and gammaSN.
Li
Qiao-Xin
QX
Department of Pathology, The University of Melbourne, and The Mental Health Research Institute of Victoria, Parkville, Vic 3010, Australia. qiao@unimelb.edu.au
Campbell
Bruce C V
BC
McLean
Catriona A
CA
Thyagarajan
Domimic
D
Gai
Wei-Ping
WP
Kapsa
Robert M
RM
Beyreuther
Konrad
K
Masters
Colin L
CL
Culvenor
Janetta G
JG
D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism*; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D006801:Humans; D009419:Nerve Tissue Proteins / Q000097:blood*; D010300:Parkinson Disease / Q000097:blood / Q000175:diagnosis*; D011237:Predictive Value of Tests; D012016:Reference Values; D051843:Synucleins; D051844:alpha-Synuclein
D015415:Biomarkers; D009419:Nerve Tissue Proteins; C497604:SNCA protein, human; D051843:Synucleins; D051844:alpha-Synuclein