Characterization of recombinant monoclonal IgA anti-PDC-E2 autoantibodies derived from patients with PBC.
Primary biliary cirrhosis (PBC) is an autoimmune liver disease characterized by the presence of autoantibodies to mitochondria (AMA). Recent evidence suggests that PBC develops after a locally driven response in the mucosa, where immunoglobulin A (IgA) is the dominant antibody isotype. In this study, we produced recombinant pyruvate dehydrogenase complex (PDC-E2)-specific dimeric human IgA monoclonal antibodies (mAbs) in a baculovirus expression system. By using 2 anti-PDC-E2 IgG mAbs derived from patients with PBC, we constructed 2 recombinant baculoviruses, each containing heavy chains with the Calpha constant region. These were simultaneously co-infected into Sf9 insect cells with recombinant baculovirus containing the J chain. A sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) immunoblotting profile of the IgA using a 6% nonreducing gel verified the dimeric nature of the autoantibodies. Both recombinants retained their original specificity for PDC-E2. In addition, the antibody showed a mitochondrial staining pattern in HEp2 cells and apically stained the biliary epithelial cells (BECs) in the liver of a patient with PBC but not a normal patient. Transcytosis experiments performed using human polymeric immunoglobulin receptor (pIgR) expressing Madine-Darby canine kidney (MDCK) cells showed that one of the recombinants showed a high degree of colocalization with PDC-E2. In conclusion, these data provide further support of the hypothesis that PDC-E2-specific IgA may enter biliary epithelial cells of PBC patients via the pIgR and complex with PDC-E2, thereby potentially contributing to the pathology of BECs. Moreover, this recombinant PDC-E2-specific mAb provides a tool for further determination of the role of anti-PDC-E2 IgA in the pathogenesis of PBC.
Fukushima
Nobuyoshi
N
Division of Rheumatology/Allergy and Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
Nalbandian
Greg
G
Van De Water
Judy
J
White
Kandra
K
Ansari
Aftab A
AA
Leung
Patrick
P
Kenny
Thomas
T
Kamita
Shizuo G
SG
Hammock
Bruce D
BD
Coppel
Ross L
RL
Stevenson
Freida
F
Ishibashi
Hiromi
H
Gershwin
M Eric
ME
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D004847:Epithelial Cells / Q000166:cytology / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000235:genetics / Q000276:immunology*; D007150:Immunohistochemistry; D008105:Liver Cirrhosis, Biliary / Q000276:immunology*; D011768:Pyruvate Dehydrogenase Complex / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D007070:Immunoglobulin A; D011768:Pyruvate Dehydrogenase Complex; D011994:Recombinant Proteins; D051047:Dihydrolipoyllysine-Residue Acetyltransferase