title: Detection by PCR of the Tyzzer's disease organism (Clostridium piliforme) in feces.
abstract: We examined whether the Tyzzer's disease organism, Clostridium piliforme, could be detected in feces by PCR. If the organism could be detected in feces, a diagnosis could be made without sacrifice of the animal. Using the RT strain of C. piliforme, we found that a C. piliforme band could be detected when there were > or = 1 x 10(0) bacteria present in the PCR solution, but the presence of fecal extract in the solution depressed the sensitivity 10 fold. Nevertheless, we could detect the C. piliforme-specific band in fecal extracts from rats in a naturally infected colony, and concluded that the use of PCR to detect C. piliforme DNA in fecal extracts would be a useful diagnostic technique.
authors.affiliation: Research Facilities for Laboratory Animal Science, Hiroshima University, School of Medicine, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Furukawa
authors.forename: Kayo
authors.initials: K
authors.lastname: Furumoto
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Fujieda
authors.forename: Eiko
authors.initials: E
authors.lastname: Okada
mesh_terms: D000818:Animals; D003013:Clostridium / Q000235:genetics / Q000302:isolation & purification*; D003015:Clostridium Infections / Q000175:diagnosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D005243:Feces / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D051381:Rats; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial