title: Selective depression of interferon-gamma and granulysin production with increase of proliferative response by Vgamma9/Vdelta2 T cells in children with tuberculosis.
abstract: Vgamma9/Vdelta2 T cells can contribute to protective immune response against Mycobacterium tuberculosis, although the extent to which and mechanisms by which they could actually protect against human tuberculosis remain unclear. We have previously reported that Vgamma9/Vdelta2 T cells from tuberculin purified protein derivative (PPD)-positive children, either healthy or affected by different clinical forms of tuberculosis, strongly proliferate to different phosphoantigens in vitro, whereas Vgamma9/Vdelta2 T cells from PPD-negative healthy subjects proliferate very poorly. We report here that Vgamma9/Vdelta2 T cells from tuberculous children have an increased proliferative activity, but decreased interferon (IFN)-gamma production and granulysin expression. After successful chemotherapy, the Vgamma9/Vdelta2 T cell proliferative response strongly decreased, whereas IFN-gamma and granulysin production consistently increased. Disease-associated changes in Vgamma9/Vdelta2 T cell effector functions in patients with tuberculosis are consistent with the possibility that these T cells may play a protective role in immune response against M. tuberculosis infection.
authors.affiliation: Dipartimento di Biopatologia, Università di Palermo, 90134 Palermo, Italy. dieli@unipa.it
authors.forename: Francesco
authors.initials: F
authors.lastname: Dieli
authors.forename: Guido
authors.initials: G
authors.lastname: Sireci
authors.forename: Nadia
authors.initials: N
authors.lastname: Caccamo
authors.forename: Caterina
authors.initials: C
authors.lastname: Di Sano
authors.forename: Lucina
authors.initials: L
authors.lastname: Titone
authors.forename: Amelia
authors.initials: A
authors.lastname: Romano
authors.forename: Paola
authors.initials: P
authors.lastname: Di Carlo
authors.forename: Annalisa
authors.initials: A
authors.lastname: Barera
authors.forename: Antonia
authors.initials: A
authors.lastname: Accardo-Palumbo
authors.forename: Alan M
authors.initials: AM
authors.lastname: Krensky
authors.forename: Alfredo
authors.initials: A
authors.lastname: Salerno
mesh_terms: D000293:Adolescent; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis; D000995:Antitubercular Agents / Q000627:therapeutic use; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007371:Interferon-gamma / Q000096:biosynthesis; D008297:Male; D009169:Mycobacterium tuberculosis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*; D014374:Tuberculin Test; D014376:Tuberculosis / Q000097:blood / Q000188:drug therapy / Q000276:immunology*
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D000995:Antitubercular Agents; C075005:GNLY protein, human; D016692:Receptors, Antigen, T-Cell, gamma-delta; D007371:Interferon-gamma