Investigations into the regulation of lipid biosynthesis in Brassica napus using antisense down-regulation.
We have generated antisense plants of Brassica napus, targeting either the cytoplasmic Type I acetyl-CoA carboxylase or the beta-ketoacyl-acyl carrier protein reductase of the Type II dissociable fatty acid synthase, in order to investigate the importance of these components in regulating lipid metabolism in plants. Using a range of down-regulated plants, it has become clear that down-regulation of these genes also causes down-regulation of other components of lipid metabolism, at the activity, translational and transcriptional levels. These plants exhibit effects, not immediately predicted, on oil yield and carbon resource allocation in seeds.
Slabas
A R
AR
School of Biological and Biomedical Sciences, University of Durham, South Road, UK. A.R.Slabas@durham.ac.uk
White
A
A
O'hara
P
P
Fawcett
T
T
D029688:Brassica napus / Q000378:metabolism*; D015536:Down-Regulation; D005227:Fatty Acids / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006720:Homozygote; D050356:Lipid Metabolism; D008954:Models, Biological; D008956:Models, Chemical; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D010641:Phenotype
D005227:Fatty Acids; D016376:Oligonucleotides, Antisense