title: Immunoselection and adenoviral genetic modulation of human osteoprogenitors: in vivo bone formation on PLA scaffold.
abstract: The aim of this study was to examine the potential of immunoselected genetically modified human osteoprogenitors to form bone in vivo on porous PLA scaffolds. Human osteoprogenitors from bone marrow were selected using the antibody STRO-1 utilising a magnetically activated cell separation system. The STRO-1(+) fraction isolated 7% of nucleated marrow cells and increased fibroblastic colony formation by 300% and alkaline phosphatase activity by 190% over unselected marrow cell cultures. To engineer bone tissue, STRO-1(+) culture-expanded cells were transduced with AxCAOBMP-2, an adenovirus carrying the human BMP-2 gene, injected into diffusion chambers containing porous PLA scaffolds, and implanted in vivo. After 11 weeks the presence of bone mineral was observed by X-ray analysis and confirmed for mineral by von Kossa, as well as bone matrix composition by Sirius red staining, birefringence, and type I collagen immunohistochemistry. Bone formation in vivo indicates the potential of using immunoselected progenitor cells and ex vivo gene transfer with biodegradable scaffolds, for the development of protocols for the treatment of a wide variety of musculo-skeletal disorders.
authors.affiliation: University Orthopaedics, University of Southampton, SO16 6YD, Southampton, UK.
authors.forename: Daniel
authors.initials: D
authors.lastname: Howard
authors.forename: Kris
authors.initials: K
authors.lastname: Partridge
authors.forename: Xuebin
authors.initials: X
authors.lastname: Yang
authors.forename: Nicholas M P
authors.initials: NM
authors.lastname: Clarke
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Okubo
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Bessho
authors.forename: Steven M
authors.initials: SM
authors.lastname: Howdle
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Shakesheff
authors.forename: Richard O C
authors.initials: RO
authors.lastname: Oreffo
mesh_terms: D020341:Absorbable Implants; D000256:Adenoviridae / Q000235:genetics*; D000368:Aged; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000235:genetics; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D018189:Immunomagnetic Separation; D019344:Lactic Acid; D008297:Male; D051379:Mice; D008819:Mice, Nude; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D010012:Osteogenesis; D011091:Polyesters; D011108:Polymers; D013234:Stem Cells / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D014161:Transduction, Genetic; D016212:Transforming Growth Factor beta
chemical_list: D000911:Antibodies, Monoclonal; C525718:BMP2 protein, human; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011091:Polyesters; D011108:Polymers; D016212:Transforming Growth Factor beta; D019344:Lactic Acid; C033616:poly(lactide); D000469:Alkaline Phosphatase