title: Purification and characterization of the lytic activity induced by the prolate-headed bacteriophage P001 in Lactococcus lactis.
abstract: The lytic activity induced by the lactococcal bacteriophage P001 was isolated from phage lysates of Lactococcus lactis by a four-step purification procedure. Two proteins lytic for L. lactis were identified with molecular weights of 28 kDA and 8 kDa, respectively. The N-terminal amino acid sequences of the two proteins were determined and degenerated oligonucleotide probes corresponding to these sequences were synthesized. DNA hybridization experiments with phage P001-DNA and lactococcal DNA revealed that both proteins were apparently encoded by a single lysin gene located on the phage P001 genome. This was confirmed by alignment of the determined N-terminal amino acid sequences with nucleotide sequences which were deduced from cloned Lactococcus bacteriophage lysin genes.
authors.forename: S
authors.initials: S
authors.lastname: Hertwig
authors.forename: W
authors.initials: W
authors.lastname: Bockelmann
authors.forename: M
authors.initials: M
authors.lastname: Teuber
mesh_terms: D001435:Bacteriophages / Q000201:enzymology* / Q000235:genetics; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D016679:Genome, Viral; D013294:Lactococcus lactis / Q000821:virology*; D009088:Mucoproteins / Q000235:genetics*; D015345:Oligonucleotide Probes; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D009088:Mucoproteins; D015345:Oligonucleotide Probes; D014764:Viral Proteins; D015678:Viral Structural Proteins; C010393:lysin, gastropoda