title: Attenuating functions of the C terminus of lambda integrase.
abstract: The tyrosine family site-specific recombinases, in contrast to the related type I topoisomerases, which act as monomers on a single DNA molecule, rely on multi-protein complexes to synapse partner DNAs and coordinate two sequential strand exchanges involving four nicking-closing reactions. Here, we analyze three mutants of the catalytic domain of lambda integrase (Int), A241V, I353M and W350ter that are defective for normal recombination, but possess increased topoisomerase activity. The mutant enzymes can carry out individual DNA strand exchanges using truncated substrates or Holliday junctions, and they show more DNA-cleavage activity than wild-type Int on isolated att sites. Structural modeling predicts that the substituted residues may destabilize interactions between the C-terminal beta-strand (beta7) of Int and the core of the protein. The cleavage-competent state of Int requires the repositioning of the nucleophile (Y342) located on beta6 and the catalyst K235 located on the flexible beta2-beta3 loop, relative to their positions in a crystal structure of the inactive conformation. We propose that the anchoring of beta7 against the protein core restrains the movement of Tyr342 and/or Lys235, causing an attenuation of cleavage activity in most contexts. Within a bona fide recombination complex, the release of strand beta7 would allow Tyr342 and Lys235 to assume catalytically active conformations in coordination with other Int protomers in the complex. The loss of beta7 packing by misalignment or truncation in the mutant proteins described here causes a loss of regulated activity, thereby favoring DNA cleavage activity in monomeric complexes and forfeiting the coordination of strand-exchange necessary for efficient recombination.
authors.affiliation: Division of Pathology, Department of Microbiology, Pathology and Immunology, Karolinska Institutet, Huddinge University Hospital, F46, SE-141 86 Stockholm, Sweden.
authors.forename: Michael
authors.initials: M
authors.lastname: Tekle
authors.forename: David J
authors.initials: DJ
authors.lastname: Warren
authors.forename: Tapan
authors.initials: T
authors.lastname: Biswas
authors.forename: Tom
authors.initials: T
authors.lastname: Ellenberger
authors.forename: Arthur
authors.initials: A
authors.lastname: Landy
authors.forename: Simone E
authors.initials: SE
authors.lastname: Nunes-Düby
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D010582:Bacteriophage lambda / Q000201:enzymology* / Q000235:genetics; D001665:Binding Sites; D004264:DNA Topoisomerases, Type I / Q000378:metabolism; D004278:DNA, Superhelical / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000821:virology; D006860:Hydrogen Bonding; D019426:Integrases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008956:Models, Chemical; D008958:Models, Molecular; D009154:Mutation; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010641:Phenotype; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D011995:Recombination, Genetic; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D004278:DNA, Superhelical; D010446:Peptide Fragments; D011994:Recombinant Proteins; D019426:Integrases; D004264:DNA Topoisomerases, Type I