Apoptosis induction by wheat-flour sphingoid bases in DLD-1 human colon cancer cells.
The apoptotic effects of plant sphingoid bases prepared from wheat-flour cerebroside on human colorectal cancer DLD-1 cells were examined. The viability of DLD-1 cells treated with such plant sphingoid bases was reduced in a dose-dependent manner and was similar to that of cells treated with sphingosine. Morphological changes such as condensed chromatin fragments were found, so those sphingoid bases reduced cell viability through causing apoptosis in these cells.
Sugawara
Tatsuya
T
Laboratory of Molecular Biodynamics, Graduate School of Life Science and Agriculture, Tohoku University, Sendai 981-8555, Japan.
Kinoshita
Mikio
M
Ohnishi
Masao
M
Miyazawa
Teruo
T
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure; D002851:Chromatography, High Pressure Liquid; D003110:Colonic Neoplasms / Q000473:pathology*; D004273:DNA, Neoplasm / Q000187:drug effects; D005433:Flour / Q000032:analysis*; D006801:Humans; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014908:Triticum / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D002843:Chromatin; D004273:DNA, Neoplasm; D013110:Sphingosine