SUT1 suppresses sec14-1 through upregulation of CSR1 in Saccharomyces cerevisiae.
SUT1 constitutive expression in aerobiosis suppressed the ts phenotype of the sec14-1 mutation, restored growth of the sec14-null mutant and corrected the translocation defect of the vacuolar carboxypeptidase Y. Therefore SUT1 was shown to be a novel potent sec14-1 suppressor. Further, the hypoxic gene CSR1 (YLR380W), a Sec14 homolog, was upregulated upon SUT1 constitutive expression. In addition, SUT1 effects on both sec14-1 suppression and on free sterol composition were abolished in a csr1-null background, showing that this gene acts downstream of SUT1.
Régnacq
Matthieu
M
Laboratoire de Génétique de la Levure, CNRS-UMR 6161, Université de Poitiers, 40 avenue du Recteur Pineau, 86022 Cedex, Poitiers, France.
Ferreira
Thierry
T
Puard
Julien
J
Bergès
Thierry
T
D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D016153:Genes, Suppressor / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics*; D010641:Phenotype; D049294:Phospholipid Transfer Proteins; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D013261:Sterols / Q000096:biosynthesis / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
C483430:CSR1 protein, S cerevisiae; D002352:Carrier Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D012097:Repressor Proteins; C483438:SEC14 protein, S cerevisiae; C118289:SEC24 protein, S cerevisiae; C408523:SUR1 protein, Candida albicans; D029701:Saccharomyces cerevisiae Proteins; D013261:Sterols; D014157:Transcription Factors