title: Study in vitro of populating autogenous Schwann cells into chemical extracted allogenous nerve.
abstract: OBJECTIVE
  To look for an ideal substance to repair large gap of nerve defect after injury by culture of Schwann cells (Scs) and preparation of acellular allogenous nerve grafts (ANG) with chemical extraction.
  
  
  METHODS
  The double adhesion culture and Arab-c to prohibit the fibroblast growth were used to achieve high-purified Scs. Triton-x-100 and sodium deoxycholate were used to achieve ANG. Finally the Scs were microinjected into the acellural nerve grafts and cultured in vitro. The consequence was analysed.
  
  
  RESULTS
  High purified Scs and ANG were acquired, which could integrate each other well. Scs could survive and transfer to aline in vitro.
  
  
  CONCLUSIONS
  Populating Scs into chemical extracted ANG may be an ideal substance to repair the large gap of nerve defect after injury.
authors.affiliation: Department of Microsurgery, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510089, China.
authors.forename: Jianyun
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Liu
authors.forename: Jiakai
authors.initials: J
authors.lastname: Zhu
authors.forename: Wenping
authors.initials: W
authors.lastname: Li
authors.forename: Jianping
authors.initials: J
authors.lastname: Xiang
mesh_terms: D000818:Animals; D017690:Cell Transplantation; D002478:Cells, Cultured; D006085:Graft Survival; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D023421:Models, Animal; D009416:Nerve Regeneration / Q000502:physiology*; D010525:Peripheral Nerves / Q000601:surgery; D051381:Rats; D011916:Rats, Inbred F344; D012583:Schwann Cells / Q000637:transplantation*; D012584:Sciatic Nerve / Q000637:transplantation; D012680:Sensitivity and Specificity; D016378:Tissue Transplantation; D014184:Transplantation, Homologous