title: HBV C promoter Sp1 binding sequence functionally substitutes for the yeast ARS1 ABF1 binding site.
abstract: Transcriptional factors have been implicated in eukaryotic DNA replication. We have studied the potential function of a viral promoter sequence in DNA replication. The hepatitis B virus (HBV) pregenomic promoter is regulated by two enhancers and cis-elements. The G-C rich region between 1734-1754 nt, which contains two SP1 binding sites, is necessary for transcription origin and HBV replication. We found that the Abf1-binding B3 element in yeast ARS1 can be functionally replaced by the viral Sp1-binding DNA sequence, which activates transcription from the HBV C promoter. Further, yeast RAP1 bound to the viral Sp1 binding sites in vitro. These results suggest that RAP1 binds to the Sp1 binding sites and stimulates yeast DNA replication.
authors.affiliation: Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
authors.forename: Peijun
authors.initials: P
authors.lastname: Yan
authors.forename: Xicheng
authors.initials: X
authors.lastname: Mao
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Xiliang
authors.initials: X
authors.lastname: Zha
authors.forename: Changde
authors.initials: C
authors.lastname: Lu
mesh_terms: D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D004261:DNA Replication / Q000235:genetics; D004271:DNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D000089804:Shelterin Complex; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D034501:Telomere-Binding Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: C061886:ABF1 protein, S cerevisiae; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; C440829:RAP1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D000089804:Shelterin Complex; D016329:Sp1 Transcription Factor; D034501:Telomere-Binding Proteins; D014157:Transcription Factors