title: PF4, a FMRFamide-related peptide, gates low-conductance Cl(-) channels in Ascaris suum.
abstract: Here we describe the actions of the peptide Lys-Pro-Asn-Phe-Ile-Arg-Phe-NH(2), or PF4, on inside-out membrane patches (n=164), recorded from vesicles derived from Ascaris suum somatic muscle cells. We observed numerous, small-amplitude Cl(-) channels in the membrane patches. The conductance of the Cl(-) channels ranged from 1.09 to 7.07 pS, the open probability (P(open)) ranged from 0.047+/-0.015 (mean+/-S.E.M.) at 0 microM PF4 to 0.156+/-0.026 at 0.1 microM PF4. The channel mean open time was more variable and prolonged at negative potentials than when the membrane patch was clamped at positive potentials: at 0.03 microM PF4, the mean open time (+/-S.E.M) at -80 mV was 522+/-333 ms; at+80 mV, it was 25+/-7 ms. When patches were isolated from the parent vesicle, there were no changes in channel characteristics, suggesting that the channels function without the involvement of cytoplasmic components. Similarly, the channel characteristics were not affected by the G-protein inhibitor, guanosine-5'-O-(2-thiodiphosphate), indicating that the ion channels do not require a G-protein to function. These data indicate that the PF4-activated Cl(-) channels function independently of intracellular signal transducers and are, therefore, directly gated by PF4.
authors.affiliation: Department of Preclinical Veterinary Science, University of Edinburgh, Summerhall, UK.
authors.forename: Jenny
authors.initials: J
authors.lastname: Purcell
authors.forename: Alan P
authors.initials: AP
authors.lastname: Robertson
authors.forename: David P
authors.initials: DP
authors.lastname: Thompson
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Martin
mesh_terms: D000818:Animals; D017165:Ascaris suum / Q000166:cytology / Q000502:physiology*; D018118:Chloride Channels / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D019204:GTP-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D009132:Muscles / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D009842:Oligopeptides / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D015290:Second Messenger Systems / Q000502:physiology; D013552:Swine / Q000469:parasitology; D013873:Thionucleotides / Q000494:pharmacology
chemical_list: D018118:Chloride Channels; C093065:KPNFIRFamide; D009842:Oligopeptides; D013873:Thionucleotides; D006153:Guanosine Diphosphate; C023427:guanosine 5'-O-(2-thiodiphosphate); D019204:GTP-Binding Proteins