title: Regulation of chromatin remodeling by inositol polyphosphates.
abstract: Chromatin remodeling is required for efficient transcription of eukaryotic genes. In a genetic selection for budding yeast mutants that were defective in induction of the phosphate-responsive PHO5 gene, we identified mutations in ARG82/IPK2, which encodes a nuclear inositol polyphosphate kinase. In arg82 mutant strains, remodeling of PHO5 promoter chromatin is impaired, and the adenosine triphosphate-dependent chromatin-remodeling complexes SWI/SNF and INO80 are not efficiently recruited to phosphate-responsive promoters. These results suggest a role for the small molecule inositol polyphosphate in the regulation of chromatin remodeling and transcription.
authors.affiliation: Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0448, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Steger
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Haswell
authors.forename: Aimee L
authors.initials: AL
authors.lastname: Miller
authors.forename: Susan R
authors.initials: SR
authors.lastname: Wente
authors.forename: Erin K
authors.initials: EK
authors.lastname: O'Shea
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D007295:Inositol Phosphates / Q000378:metabolism*; D009154:Mutation; D009687:Nuclear Proteins; D028061:Phosphate Transport Proteins / Q000235:genetics* / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D028081:Proton-Phosphate Symporters / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D004718:Saccharomycetales / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D007295:Inositol Phosphates; D009687:Nuclear Proteins; D028061:Phosphate Transport Proteins; D028081:Proton-Phosphate Symporters; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C013571:inositol pentaphosphate; C055831:phosphate permease; C425775:ARG82 protein, S cerevisiae; D017853:Phosphotransferases (Alcohol Group Acceptor); C051038:Inositol 1,4,5-trisphosphate 3-kinase; D000251:Adenosine Triphosphatases