title: Monitoring the expression pattern of around 7,000 Arabidopsis genes under ABA treatments using a full-length cDNA microarray.
abstract: Full-length cDNAs are essential for functional analysis of plant genes. Recently, cDNA microarray analysis has been developed for quantitative analysis of global and simultaneous analysis of expression profiles. Microarray technology is a powerful tool for identifying genes induced by environmental stimuli or stress and for analyzing their expression profiles in response to environmental signals. We prepared an Arabidopsis full-length cDNA microarray containing around 7,000 independent full-length cDNA groups and analyzed the expression profiles of genes. The transcripts of 245, 299, 54 and 213 genes increased after abscisic acid (ABA), drought-, cold-, and salt-stress treatments, respectively, with inducibilities more than fivefold compared with those of control genes [corrected]. The cDNA microarray analysis showed that many ABA-inducible genes were induced after drought- and high-salinity-stress treatments, and that there is more crosstalk between drought and ABA responses than between ABA and cold responses. Among the ABA-inducible genes identified, we identified 22 transcription factor genes, suggesting that many transcriptional regulatory mechanisms exist in the ABA signal transduction pathways.
authors.affiliation: Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba 305-0074, Japan.
authors.forename: Motoaki
authors.initials: M
authors.lastname: Seki
authors.forename: Junko
authors.initials: J
authors.lastname: Ishida
authors.forename: Mari
authors.initials: M
authors.lastname: Narusaka
authors.forename: Miki
authors.initials: M
authors.lastname: Fujita
authors.forename: Tokihiko
authors.initials: T
authors.lastname: Nanjo
authors.forename: Taishi
authors.initials: T
authors.lastname: Umezawa
authors.forename: Asako
authors.initials: A
authors.lastname: Kamiya
authors.forename: Maiko
authors.initials: M
authors.lastname: Nakajima
authors.forename: Akiko
authors.initials: A
authors.lastname: Enju
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakurai
authors.forename: Masakazu
authors.initials: M
authors.lastname: Satou
authors.forename: Kenji
authors.initials: K
authors.lastname: Akiyama
authors.forename: Kazuko
authors.initials: K
authors.lastname: Yamaguchi-Shinozaki
authors.forename: Piero
authors.initials: P
authors.lastname: Carninci
authors.forename: Jun
authors.initials: J
authors.lastname: Kawai
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Hayashizaki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics*; D003080:Cold Temperature; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020411:Oligonucleotide Array Sequence Analysis; D009997:Osmotic Pressure; D011401:Promoter Regions, Genetic
chemical_list: D000040:Abscisic Acid