Production of patchouli mild mosaic virus resistant patchouli plants by genetic engineering of coat protein precursor gene.
Patchouli (Pogostemon cablin (Blanco) Benth), an aromatic crop which yields an essential oil, is widely cultivated in South-east Asia. Patchouli mild mosaic virus (PaMMV) infects patchouli plants and causes decrease in leaf biomass and essential oil yield. Transgenic patchouli plants with PaMMV coat protein precursor (CP-P) gene have been produced by Agrobacterium-mediated transformation. PaMMV CP-P gene integration into the patchouli genome was confirmed by the PCR method and by Southern blot analysis. The transformants were estimated to contain one to three copy genes using Southern blot analysis. The transformant with three copy genes was tested for the resistance to PaMMV by artificially inoculating plants grown in an environmentally controlled cabinet, and this transformant was found to be highly resistant to PaMMV. The transgenic patchouli plant with PaMMV CP-P gene should provide valuable material for protecting against PaMMV.
Kadotani
Naoto
N
Department of Bioscience, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan. kadotani@nodai.ac.jp
Ikegami
Masato
M
D015139:Blotting, Southern; D036022:Capsid Proteins / Q000235:genetics*; D046508:Culture Techniques; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D007113:Immunity, Innate / Q000235:genetics; D019686:Lamiaceae / Q000235:genetics* / Q000821:virology; D009029:Mosaic Viruses / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D010942:Plant Viruses / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics
D036022:Capsid Proteins