Genomic organization of the Papaver rhoeas self-incompatibility S(1) locus.
The self-incompatibility (SI) response in Papaver rhoeas depends upon the cognate interaction between a pollen-expressed receptor and a stigmatically expressed ligand. The genes encoding these components are situated within the S-locus. In order for SI to be maintained, the genes encoded by the S-locus must be co-inherited with no recombination between them. Several hypotheses, including sequence heterogeneity and chromosomal position, have been put forward to explain the maintenance of the S-locus in the SI systems of the Brassicaceae and the Solanaceae. A region of the Papaver rhoeas genome encompassing part of the self-incompatibility S(1) locus has been cloned and sequenced. The clone contains the gene encoding the stigmatic component of the response, but does not contain a putative pollen S-gene. The sequence surrounding the S(1) gene contains several diverse repetitive DNA elements. As such, the P. rhoeas S-locus bears similarities to the S-loci of other SI systems. An attempt to localize the P. rhoeas S-locus using fluorescence in situ hybridization (FISH) has also been made. The potential relevance of the findings to mechanisms of recombination suppression is discussed.
Wheeler
M J
MJ
Wolfson Laboratory for Plant Molecular Biology, School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. m.wheeler@bham.ac.uk
Armstrong
S A
SA
Franklin-Tong
V E
VE
Franklin
F C H
FC
D000595:Amino Acid Sequence; D005298:Fertility / Q000235:genetics; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008969:Molecular Sequence Data; D010207:Papaver / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping / Q000379:methods; D012260:Ribonucleases / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
C099641:PRS3S protein, Papaver rhoeas; D010940:Plant Proteins; C402744:SBP protein, Papaver rhoeas; D012260:Ribonucleases; C022542:ribonuclease S