[In vitro experiment of inhibiting cell proliferation on human esophageal squamous cell cancer T. Tn cell line by proliferating cell nuclear antigen antisense oligodeoxynucleotide].
OBJECTIVE
The high expression of proliferation cell nuclear antigen were found in the patients with esophageal carcinoma in poor prognosis. The aim of this study was to investigate the effect in vitro of antisense oligodeoxynucleotide (ASODN) of PCNA on its gene expression and cell proliferation in human esophageal squamous cell cancer T. Tn cell line.


METHODS
Phosphorothioate PCNA ASODN and sense oligodeoxynucleotide (SODN) were synthesized and transfected to T. Tn cells mediated by dosper liposomal. Their effects on cell proliferation were examined by MTT method, 3H-TdR method, immunohistochemical study, and flow cytometer (FCM).


RESULTS
PCNA ASODN could significantly inhibit the growth of T. Tn cell lines. The SODN and control lipsome showed no such effect. The inhibition peaked at 24 hour after transfection, the inhibition rate reached 63.3% at 0.2 mumol/L by MTT method, the inhibition effect was dose-dependent. ASODN could downregulate the expression levels of PCNA protein. And PCNA ASODN transfection could inhibit the transition period of G0/G1 phase to S phase.


CONCLUSIONS
The data suggested that ASODN could specific inhibit the expression of PCNA protein and regulate cell cycle and cell proliferation in T. Tn cells and antisense therapy could be an useful method to improve prognosis in ESCC.
Yang
Lin
L
Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, P. R. China. yanglin70@yahoo.com
Ye
Shi-duo
SD
Feng
Lun-gao
LG
Wang
Jia-shun
JS
Zhai
Wei
W
D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis / Q000187:drug effects; D004938:Esophageal Neoplasms / Q000473:pathology*; D006801:Humans; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000187:drug effects* / Q000235:genetics; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D020319:Oligodeoxyribonucleotides, Antisense; D018809:Proliferating Cell Nuclear Antigen