title: Does growth hormone treatment alter skeletal muscle mitochondrial respiration in rats?
abstract: OBJECTIVE
  Growth hormone (GH) has been shown to stimulate lipolysis and enhance lipid oxidation. We investigated whether GH could improve mitochondrial oxidative capacity.
  
  
  METHODS
  Fourteen male Wistar rats received 14-day treatment with biosynthetic human GH (10 IU/kg/24 h) or placebo. Mitochondria were isolated from the total muscle of one hind limb of the rat. Mitochondrial oxygen consumption was measured in vitro using a Clark-type electrode with three substrates: palmitoyl-L-carnitine, pyruvate and succinate (+ rotenone).
  
  
  RESULTS
  Muscle mitochondrial yield was not significantly different in the GH-treated group from that in controls. Neither the basal nor ADP-stimulated respiratory state reached a significant difference between the 2 groups with palmitoyl-L-carnitine, pyruvate, and succinate.
  
  
  CONCLUSIONS
  GH treatment did not improve the oxidative capacity of skeletal muscle mitochondria.
authors.affiliation: Département de Physiologie, Laboratoire de Physiologie des Interactions, UPRES EA701, Institut de Biologie, Faculté de Médecine, Montpellier, France. c.peyreigne@laposte.net
authors.forename: C
authors.initials: C
authors.lastname: Peyreigne
authors.forename: E
authors.initials: E
authors.lastname: Raynaud
authors.forename: C
authors.initials: C
authors.lastname: Fedou
authors.forename: C
authors.initials: C
authors.lastname: Prefaut
authors.forename: J-F
authors.initials: JF
authors.lastname: Brun
authors.forename: J
authors.initials: J
authors.lastname: Mercier
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D050356:Lipid Metabolism; D008297:Male; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000378:metabolism*; D009124:Muscle Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009124:Muscle Proteins; D000244:Adenosine Diphosphate; D013006:Growth Hormone; D009245:NADH Dehydrogenase