title: Preliminary crystallographic studies of two C-terminally truncated copper-containing nitrite reductases from Achromobacter cycloclastes: changed crystallizing behaviors caused by residue deletion.
abstract: The C-terminal segment of copper-containing nitrite reductase from Achromobacter cycloclastes (AcNiR) has been found essential for maintaining both the quaternary structure and the enzyme activity of AcNiR. C-terminal despentapeptide AcNiR (NiRc-5) and desundecapeptide AcNiR (NiRc-11) are two important truncated mutants whose activities and stability have been affected by residue deletion. In this study, the two mutants were crystallized using the hanging drop vapor diffusion method. Crystals of NiRc-5 obtained at pH 5.0 and 6.2 both belonged to the P2(1)2(1)2(1) space group with unit cell parameters a=99.0 A, b=117.4 A, c=122.8 A (pH 5.0) and a=98.9A, b=117.7A, c=123.0A (pH 6.2). NiRc-11 was crystallized in two crystal forms: the tetragonal form belonged to the space group P4(1) with a=b=96.0A and c=146.6A; the monoclinic form belonged to the space group P2(1) with a=86.0A, b=110.1A, c=122.7A, and beta=101.9 degrees. The crystallizing behaviors of the two mutants differed from that of the native enzyme. Such change in combination with residue deletion is also discussed here.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, 100101, Beijing, China.
authors.forename: Hai Tao
authors.initials: HT
authors.lastname: Li
authors.forename: Tschining
authors.initials: T
authors.lastname: Chang
authors.forename: Ming Yih
authors.initials: MY
authors.lastname: Liu
authors.forename: Jean
authors.initials: J
authors.lastname: Le Gall
authors.forename: Xiao Min
authors.initials: XM
authors.lastname: An
authors.forename: Lu Lu
authors.initials: LL
authors.lastname: Gui
authors.forename: Ji Ping
authors.initials: JP
authors.lastname: Zhang
authors.forename: Dong Cai
authors.initials: DC
authors.lastname: Liang
authors.forename: Wen Rui
authors.initials: WR
authors.lastname: Chang
mesh_terms: D000421:Alcaligenes / Q000201:enzymology*; D003300:Copper / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D009572:Nitrite Reductases / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D017384:Sequence Deletion
chemical_list: D003300:Copper; D009572:Nitrite Reductases