title: 11beta-hydroxylase and aldosterone synthase expression in fetal rat hippocampal neurons.
abstract: The central nervous system produces many of the enzymes responsible for corticosteroid synthesis. A model system to study the regulation of this local system would be valuable. Previously, we have shown that primary cultures of hippocampal neurons isolated from the fetal rat can perform the biochemical reactions associated with the enzymes 11beta-hydroxylase and aldosterone synthase. Here, we demonstrate directly that these enzymes are present within primary cultures of fetal rat hippocampal neurons.
authors.affiliation: Department of Medicine and Therapeutics, Western Infirmary, Church Street, Glasgow G11 6NT, UK. 924473m@clinmed.gla.ac.uk
authors.forename: S M
authors.initials: SM
authors.lastname: MacKenzie
authors.forename: M
authors.initials: M
authors.lastname: Lai
authors.forename: C J
authors.initials: CJ
authors.lastname: Clark
authors.forename: R
authors.initials: R
authors.lastname: Fraser
authors.forename: C E
authors.initials: CE
authors.lastname: Gómez-Sánchez
authors.forename: J R
authors.initials: JR
authors.lastname: Seckl
authors.forename: J M C
authors.initials: JM
authors.lastname: Connell
authors.forename: E
authors.initials: E
authors.lastname: Davies
mesh_terms: D000311:Adrenal Glands / Q000196:embryology / Q000201:enzymology; D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics; D019405:Cytochrome P-450 CYP11B2 / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D006624:Hippocampus / Q000166:cytology / Q000196:embryology* / Q000201:enzymology*; D009474:Neurons / Q000201:enzymology*; D010750:Phosphoproteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013252:Steroid 11-beta-Hydroxylase / Q000235:genetics / Q000378:metabolism*; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D010750:Phosphoproteins; D012333:RNA, Messenger; D000098682:Steroidogenic Acute Regulatory Protein; D019405:Cytochrome P-450 CYP11B2; D013252:Steroid 11-beta-Hydroxylase; D002786:Cholesterol Side-Chain Cleavage Enzyme