ACTH(1-24) down-regulates expression of ciliary neurotrophic factor mRNA in cultured rat astrocyte.
We examined the effects on astrocytes of ACTH, which is used to treat West syndrome. We stimulated cultured rat astrocytes with ACTH(1-24), corticotropin-releasing factor, and dexamethasone, and examined changes in neurotrophic factor mRNAs by reverse transcription-PCR. Down-regulation of ciliary neurotrophic factor mRNA expression was observed by stimulation with ACTH(1-24), but the expression of nerve growth factor, brain-derived neurotrophic factor, and nerotrophin-3 mRNAs was unaffected. Northern blot analysis revealed that the decrease in ciliary neurotrophic factor mRNA occurred 4 h after stimulation with more than 10 nM of ACTH(1-24). Up-regulation of nerotrophin-3 mRNA expression was found after stimulation with 1 mM dexamethasone. These results suggest that ACTH(1-24) administrated in West syndrome may influence the expression of neurotrophic factors in astrocytes in vivo.
Kokubo
Minoru
M
Department of Pediatrics, Neonatology and Congenital Disorders, Nagoya City University Graduate School of Medical Sciences, Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.
Asai
Kiyofumi
K
Yamamoto
Naoki
N
Aoyama
Mineyoshi
M
Morikawa
Masayuki
M
Togari
Hajime
H
Wada
Yoshiro
Y
Kato
Taiji
T
D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism*; D001483:Base Sequence; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics; D002478:Cells, Cultured; D020934:Ciliary Neurotrophic Factor / Q000235:genetics*; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology; D003366:Cosyntropin / Q000494:pharmacology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D004247:DNA / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007223:Infant; D020932:Nerve Growth Factor / Q000235:genetics; D020933:Neurotrophin 3 / Q000235:genetics; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013036:Spasms, Infantile / Q000188:drug therapy / Q000378:metabolism
D019208:Brain-Derived Neurotrophic Factor; D020934:Ciliary Neurotrophic Factor; D004791:Enzyme Inhibitors; D020933:Neurotrophin 3; D012333:RNA, Messenger; D003366:Cosyntropin; D003907:Dexamethasone; D004247:DNA; D003346:Corticotropin-Releasing Hormone; D020932:Nerve Growth Factor; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C