Strain differentiation of Indian isolates of Salmonella by ERIC-PCR.
Enterobacterial repetitive intergenic consensus (ERIC) sequences are the repetitive elements present in the family enterobacteriacae. In the present study, ERIC-PCR (target ERIC sequence) was used for the molecular typing of 24 isolates of Salmonella serovars, namely abortusequi, choleraesuis, bareilly and dublin. In ERIC-PCR, seven molecular types were observed with ERIC-Cl primer, and nine molecular types with ERIC-C2 primer. When the results of both the ERIC-PCR were combined for molecular typing, 21 molecular types were observed, which indicated a high degree of discrimination. Both the ERIC primers are designed from the ERIC consensus sequence, yet they gave different profiles, indicating that they supplement each other. ERIC sequences were found to be useful targets for molecular typing. The different profiles observed appear to be due to differences in ERIC sequences and differences in inter-ERIC distance. The study indicates that ERIC-PCR is a very efficient tool for molecular typing of Salmonella species.
Saxena
M K
MK
Division of Veterinary Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, India.
Singh
V P
VP
Lakhcharua
B D
BD
Taj
G
G
Sharma
B
B
D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D007194:India; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012475:Salmonella / Q000737:chemistry / Q000145:classification* / Q000235:genetics
D004269:DNA, Bacterial