title: Isulinotropic properties of Nigella sativa oil in Streptozotocin plus Nicotinamide diabetic hamster.
abstract: The present study was designed to investigate the possible insulinotropic properties of Nigella sativa L. (N. sativa) oil in Streptozotocin plus Nicotinamide-induced diabetes mellitus in hamsters. Nicotinamide was injected intraperitoneally 15min before injection of Streptozotocin intravenously. Oral treatment with N. sativa oil began 4 weeks after induction of diabetes. Serum insulin was measured by enzymeimmunoassay. Islets insulin was stained using anti-insulin monoclonal antibody. Significant decrease in blood glucose level together with significant increase in serum insulin level were observed after treatment with N. sativa oil for 4 weeks. Big areas with positive immuno-reactivity for the presence of insulin were observed in the pancreases from N. sativa oil-treated group compared to non-treated one using immunohistochemical staining. Therefore, our data show that the hypoglycemic effect of N. sativa oil in Streptozotocin plus Nicotinamide diabetic hamsters resulted, at least partly, from a stimulatory effect on beta cell function with consequent increase in serum insulin level. These results indicate that N. sativa oil has insulinotropic properties in type 2-like model.
authors.affiliation: Department of Physiology and Pathogenetic Veterinary Sciences, The United Graduate School, Gifu University, Japan.
authors.forename: K M
authors.initials: KM
authors.lastname: Fararh
authors.forename: Y
authors.initials: Y
authors.lastname: Atoji
authors.forename: Y
authors.initials: Y
authors.lastname: Shimizu
authors.forename: T
authors.initials: T
authors.lastname: Takewaki
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D006224:Cricetinae; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy*; D007150:Immunohistochemistry; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D009536:Niacinamide / Q000008:administration & dosage; D010179:Pancreas / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology*; D013311:Streptozocin / Q000008:administration & dosage; D029974:Carum
chemical_list: D001786:Blood Glucose; D007328:Insulin; D010938:Plant Oils; D009536:Niacinamide; D013311:Streptozocin; C104525:caraway oil