Serum paraoxonase activity and paraoxonase gene polymorphism in type 2 diabetic patients with or without vascular complications.
BACKGROUND
Serum paraoxonase (PON) activity and the relevance of PON gene polymorphism in vascular complications of type 2 diabetic patients were investigated in a case-control study.


METHODS
The population included 105 control subjects, 96 diabetic patients without vascular complications and 71 diabetics with vascular complications.


RESULTS
Serum PON activity was significantly decreased (p<0.001) in diabetic patients without vascular complications: 207 IU (25-817) compared with the controls: 259 IU (24-950). Although serum PON activity was also decreased: 232 IU (34-797) in the population with vascular complications, the difference was not statistically significant (p=0.11). The Q192 allele frequency is significantly higher (p<0.005) in diabetics without vascular complications (77%), and with vascular complications (73%) than in the controls (63%). No significant association was found between either PON(1)55 L/M and PON(2)311 C/S gene polymorphisms and vascular complications.


CONCLUSIONS
The difference in allele frequency for the PON(1) Q/R 192 gene polymorphism may be the cause of the low paraoxonase activity observed in type 2 diabetes mellitus. Further studies need to be conducted to elucidate the role of the enzyme in the development of vascular complications in diabetes.
Letellier
C
C
Laboratoire de Biochimie, Hôpital Sud, CHU Rennes, France.
Durou
M R
MR
Jouanolle
A M
AM
Le Gall
J Y
JY
Poirier
J Y
JY
Ruelland
A
A
D000328:Adult; D000483:Alleles; D043303:Aryldialkylphosphatase; D001483:Base Sequence; D015992:Body Mass Index; D016022:Case-Control Studies; D017931:DNA Primers; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology / Q000235:genetics*; D003925:Diabetic Angiopathies / Q000201:enzymology / Q000235:genetics; D004950:Esterases / Q000097:blood* / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012016:Reference Values
D017931:DNA Primers; D004950:Esterases; D043303:Aryldialkylphosphatase