In-vitro analysis for microbial barrier properties of 2-octyl cyanoacrylate-derived wound treatment films.
BACKGROUND
In recent years, 2-octyl cyanoacrylate monomer has been formulated for various wound care products that have been approved by the Food and Drug Administration (FDA).


OBJECTIVE
To evaluate the in vitro effectiveness of 2-octyl cyanoacrylate formulation-based films as barriers to various pathogens, including bacteria, fungi, and yeast.


METHODS
The barrier properties of the cyanoacrylate films prepared by the following two methods were tested: (1) prepolymerized film and (2) in situ polymerized film. The upper surface of films was inoculated such that the microorganisms would have to penetrate the film to colonize the media beneath. Nine different organisms were used. Plates were observed for growth at two, four, and seven days after inoculation.


RESULTS
No growth was observed in any test, with the exception of prepolymerized film challenged with Pseudomonas aeruginosa. The pattern of growth observed suggests that the bacteria colonized the medium by traveling around and not through the film.


CONCLUSIONS
2-Octyl cyanoacrylate-based films are excellent microbial barriers.
Narang
Upvan
U
Closure Medical Corporation, 5250 Greens Dairy Road, Raleigh, NC 27616, USA. unarang@closuremed.com
Mainwaring
Lawrence
L
Spath
Gina
G
Barefoot
Joe
J
D001419:Bacteria / Q000187:drug effects*; D003487:Cyanoacrylates / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects*; D066298:In Vitro Techniques; D009779:Occlusive Dressings / Q000382:microbiology*; D014014:Tissue Adhesives / Q000494:pharmacology*; D016896:Treatment Outcome
D003487:Cyanoacrylates; D014014:Tissue Adhesives; C100832:octyl 2-cyanoacrylate