[Expression of energy metabolism-related genes in myocardial tissues of rats with diabetic cardiomyopathy].
OBJECTIVE
To study the pathogenesis of diabetic cardiomyopathy using DNA microarray.


METHODS
Experimental rats were grouped into a diabetic cardiomyopathy and a normal control group. The cDNA probes were prepared by labeling the mRNA extracted from normal tissue and tissue of diabetic cardiomyopathy with Cy3-dUTP and Cy5-dUTP respectively through reverse transcription. DNA microarray were constructed by spotting PCR products of 4 000 rat cDNAs onto a specially treated glass slides, and were then hybridized against the cDNA probes followed by fluorescent signals scanning.


RESULTS
The expression of energy metabolism-related genes were lower in the tissues of diabetic cardiomyopathy than in normal tissue.


CONCLUSIONS
The energy metabolism disorder may play an important role in the pathogenesis of diabetic cardiomyopathy.
Chen
Gang
G
Department of Endocrinology, Fujian Provincial Hospital, Fuzhou 350001, China.
Lin
Li-Xiang
LX
Zhuang
Wei-Te
WT
Yao
Jin
J
Liang
Ji-Xin
JX
Huang
Hui-Bin
HB
Lin
Fan
F
Lin
Jian-Li
JL
D000818:Animals; D009202:Cardiomyopathies / Q000235:genetics / Q000378:metabolism*; D048909:Diabetes Complications; D003920:Diabetes Mellitus / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D004734:Energy Metabolism / Q000235:genetics*; D015870:Gene Expression; D020869:Gene Expression Profiling; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley