title: Optimization and validation of an analytical procedure by high-performance liquid chromatography for the quantification of peroxisomicines and isoperoxisomicines.
abstract: Peroxisomicine A1 is a potential antineoplastic substance extracted from plants of the genus Karwinskia. An RP-HPLC-DAD method was developed and validated for the separation and quantification of four isomers of this compound. These isomers coelute in the preparative procedure and are present at a proportion ranging between 3 and 5% in the peroxisomicine A1 purified in the laboratory. The desirability coefficient of the method described here was enhanced 140% with respect to the previously reported method.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Medicine, U.A.N.L., P.O. Box 2316, Suc. Tecnológico, 64841, Nuevo Leon, Monterrey, Mexico
authors.forename: A
authors.initials: A
authors.lastname: Osorio-Pérez
authors.forename: M
authors.initials: M
authors.lastname: Salazar-Cavazos
authors.forename: A
authors.initials: A
authors.lastname: Piñeyro-López
authors.forename: N
authors.initials: N
authors.lastname: Waksman de Torres
mesh_terms: D000873:Anthracenes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D000873:Anthracenes