[Expression of Fas in six human urogenital malignant cell lines].
OBJECTIVE
Expression of Fas in tumors is an important basis for the treatment of the patients with tumor and determination of the prognosis. This study was designed to investigate the expression of Fas in urogenital tumor cell lines.


METHODS
Using direct immunofluorescence flow cytometry, Western blot, we detected and Northern blot, the expression of Fas in six urinary malignant cell lines [bladder carcinoma(T24, EJ, BIU-87), renal carcinoma (GRC-1, RCC-949), prostatic carcinoma (PC-3M)] and one primary in vitro cultured renal fibroblast.


RESULTS
Expression of Fas in 6 urinary tumor cell lines was commonly presented but with limited positive cell percentage (range 16.51%-49.13%) and relatively lower fluorescence intensity, compared with expression of Fas in normal control of primary in vitro cultured renal fibroblast (the positive rate of Fas reached 77.98%) measured by flow cytometry with direct immunofluorescence; In detection of expression of Fas in 6 urogenital cell lines, a 48 kDa clear band was found with the strongest intensity in T24 cell line and the weakest in BIU-87. Fas mRNA from all tumor cell lines but BIU-87 was detected.


CONCLUSIONS
Fas was extensively expressed in urogenital malignant cells and normal cell. The lower expression of Fas in urinary malignant cell lines than that in normal cells might be the reason for occurrence and progression of urinary malignant tumors.
Li
Hong-jun
HJ
Daxing Male Hospital of Wu Jieping's Medical Foundation, Beijing 102600, P. R. China. hongjun63@btamail.net.cn
Yu
Li-zhang
LZ
Guo
Ying-lu
YL
Ding
Yi
Y
D015152:Blotting, Northern / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D015870:Gene Expression; D006801:Humans; D007668:Kidney / Q000166:cytology; D007680:Kidney Neoplasms / Q000235:genetics; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000235:genetics; D014565:Urogenital Neoplasms / Q000235:genetics*; D019014:fas Receptor / Q000096:biosynthesis / Q000235:genetics*
D019014:fas Receptor