title: Response of rat osteoblast-like cells to microstructured model surfaces in vitro.
abstract: The role of surface microtopography in combination with different surface wettability for rat calvaria cell differentiation was examined. Mineralization and alkaline phosphatase (ALP) activity of rat calvaria cells on flat polydimethylsiloxane (PDMS) or PDMS contained pyramids which were either hydrophilic or hydrophobic were compared. ALP expressing cells were more frequent on hydrophilic PDMS contained pyramids. ALP activity, peaked at day 9, was highest for hydrophilic pyramids followed by hydrophobic pyramids and flat hydrophilic PDMS surfaces. A similar pattern was obtained with respect to mineralized nodules. These observations showed that micro-sized surface features promote differentiation of rat calvaria cells. Further, hydrophilic surfaces are more prone to stimulate differentiation in comparison with hydrophobic surfaces. The results suggest that both material surface chemistry and topography affect osteoblast differentiation.
authors.affiliation: Department of Biomaterials, Box 412, Göteborg University, SE-405 30 Göteborg, Sweden.
authors.forename: Haihong
authors.initials: H
authors.lastname: Liao
authors.forename: Ann-Sofie
authors.initials: AS
authors.lastname: Andersson
authors.forename: Duncan
authors.initials: D
authors.lastname: Sutherland
authors.forename: Sarunas
authors.initials: S
authors.lastname: Petronis
authors.forename: Bengt
authors.initials: B
authors.lastname: Kasemo
authors.forename: Peter
authors.initials: P
authors.lastname: Thomsen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D020099:Coated Materials, Biocompatible / Q000378:metabolism*; D004129:Dimethylpolysiloxanes / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D012828:Silicones / Q000378:metabolism; D012886:Skull / Q000166:cytology; D013194:Staining and Labeling; D013499:Surface Properties; D017155:Wettability
chemical_list: D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D012828:Silicones; C013830:baysilon; D000469:Alkaline Phosphatase