title: Detection of oxidized high-density lipoprotein.
abstract: This paper reviews working procedures for the separation and detection of oxidized high-density lipoproteins (ox-HDL) and their constituents. It begins with an introductory overview of structural alterations of the HDL particle and its constituents generated during oxidation. The main body of the review delineates various procedures for the isolation and detection of ox-HDL as well as the purification and separation of phosphatidylcholine metabolites and denatured apolipoproteins in the particle. The useful methods published more recently are picked up and the utility of the separation techniques is described. The last section covers a clinical evaluation of changes in these factors in ox-HDL as well as future directions of ox-HDL research.
authors.affiliation: Department of Biochemistry, Saitama Medical School, 38 Morohongo, Moroyama, Iruma-gun, Saitama, 350-0495, Japan. t_matsu@saitama-med.ac.jp
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Matsunaga
authors.forename: Iwao
authors.initials: I
authors.lastname: Koyama
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hokari
authors.forename: Tsugikazu
authors.initials: T
authors.lastname: Komoda
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008054:Lipid Peroxides / Q000737:chemistry; D008075:Lipoproteins, HDL / Q000097:blood / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D008054:Lipid Peroxides; D008075:Lipoproteins, HDL