title: Nanostructured polymer/nanophase ceramic composites enhance osteoblast and chondrocyte adhesion.
abstract: Osteoblast (bone-forming cell) and chondrocyte (cartilage-synthesizing cell) adhesion on novel nanostructured polylactic/glycolic acid (PLGA) and titania composites were investigated in the present in vitro study. Nanostructured polymers were created by chemically treating micron-structured PLGA with select concentrations of NaOH for various periods of time. Dimensions of ceramics were controlled by utilizing either micron or nanometer grain size titania. Compared with surfaces with conventional or micron surface roughness dimensions, results provided the first evidence of increased osteoblast and chondrocyte adhesion on 100 wt% PLGA films with nanometer polymer surface roughness dimensions. Results also confirmed other literature reports of enhanced osteoblast adhesion on 100 wt% nanometer compared with conventional grain size titania compacts; however, the present study provided the first evidence that decreasing titania grain size into the nanometer range did not influence chondrocyte adhesion. Finally, osteoblast and chondrocyte adhesion increased on 70/30 wt% PLGA/titania composites formulated to possess nanosurface rather than conventional surface feature dimensions. The present study, thus, provided evidence that these nanostructured PLGA/titania composites may possess the ability to simulate surface and/or chemical properties of bone and cartilage, respectively, to allow for exciting alternatives in the design of prostheses with greater efficacy.
authors.affiliation: Deparment of Biomedical Engineering, Purdue University, 1296 Potter Building, West Lafayette, IN 49707, USA.
authors.forename: Sarina
authors.initials: S
authors.lastname: Kay
authors.forename: Anil
authors.initials: A
authors.lastname: Thapa
authors.forename: Karen M
authors.initials: KM
authors.lastname: Haberstroh
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Webster
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D002516:Ceramics / Q000737:chemistry; D019902:Chondrocytes / Q000502:physiology; D006801:Humans; D036103:Nanotechnology; D010006:Osteoblasts / Q000502:physiology; D011108:Polymers / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods
chemical_list: D011108:Polymers