Antiviral and antitumoral effects of recombinant chicken myelomonocytic growth factor in virally induced lymphoma.
Chicken myelomonocytic growth factor (cMGF) is a 27-kDa glycoprotein that stimulates the growth and activation of cells from the monocyte/macrophage lineage. Recombinant cMGF was produced in a prokaryotic (Escherichia coli) expression system and purified via a C-terminal His-tag. Treatment of 2-week-old histocompatible B(13)/B(13) chickens highly susceptible to Marek's disease (MD) with rcMGF (two daily injections of 50 microg rcMGF per chicken) enhanced background and LPS-inducible systemic NO (NO3- + NO2-) responses 3 days later. NO has antiviral activity on Marek's disease virus (MDV), a herpesvirus specifically inducing T cell-lymphomas in chickens. When the very virulent strain of MDV RBI-B was inoculated 3 days after treatment with rcMGF, MDV viremia was significantly inhibited and development of visceral tumors was drastically reduced. Combination of rcMGF with partially protective vaccination using a herpesvirus of turkey (HVT) further reducedtumor burden and significantly delayed mortality, but only in very young birds. In conclusion, rcMGF might be worth considering as a stimulator of innate immune response in chickens, such as activation of macrophages and NO production, and thus be beneficial for its antiviral and antitumoral effects in vaccination against MD lymphoma.
Djeraba
Aouatef
A
Kut
Emmanuel
E
Rasschaert
Denis
D
Quéré
Pascale
P
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D030161:Avian Proteins / Q000096:biosynthesis / Q000494:pharmacology*; D002645:Chickens / Q000502:physiology*; D016207:Cytokines; D004926:Escherichia coli / Q000378:metabolism; D005347:Fibroblasts / Q000821:virology; D026002:Herpesvirus 1, Meleagrid / Q000276:immunology; D008381:Herpesvirus 2, Gallid / Q000187:drug effects*; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008223:Lymphoma / Q000188:drug therapy* / Q000821:virology*; D008380:Marek Disease / Q000188:drug therapy* / Q000517:prevention & control / Q000821:virology*; D009569:Nitric Oxide / Q000096:biosynthesis; D011201:Poultry Diseases / Q000188:drug therapy* / Q000517:prevention & control / Q000821:virology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
D000970:Antineoplastic Agents; D000998:Antiviral Agents; D030161:Avian Proteins; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D008070:Lipopolysaccharides; C059626:MGF protein, chicken; D011994:Recombinant Proteins; D009569:Nitric Oxide