Assessment of the efficacy of Japanese Bacillus thuringiensis isolates against the cigarette beetle, Lasioderma serricorne (Coleoptera: Anobiidae).
A total of 2,652 Japanese isolates of Bacillus thuringiensis, belonging to at least 54 H serogroups, were examined for assessment of the toxicity against the cigarette beetle, Lasioderma serricorne (Coleoptera: Anobiidae). When tested with spore/parasporal inclusion mixtures, strong larvicidal activities were associated with 28 isolates (1.1%). Serologically, these toxic isolates fell into 4 known H serovars: thuringiensis (9 isolates), kurstaki (2), kenyae (2), and darmstadiensis (15). Purified parasporal inclusions of the 10 selected isolates exhibited no larvicidal activity, while the supernatants of liquid cultures showed larvicidal and/or growth inhibitory effects. The activities were fully retained after heat treatment at 100 degrees C for 10 min. Overall results suggest that beta-exotoxin (or thuringiensin)-related substances are responsible for the toxicity of the present B. thuringiensis isolates against the cigarette beetle.
Tsuchiya
Satoshi
S
Leaf Tobacco Research Center, Japan Tobacco Inc., 1900 Idei, Oyama, Tochigi 323-0808, Japan. satoshi.tsuchiya@ims.jti.co.jp
Kasaishi
Yoshihiro
Y
Harada
Haruyasu
H
Ichimatsu
Tokio
T
Saitoh
Hiroyuki
H
Mizuki
Eiichi
E
Ohba
Michio
M
D000241:Adenosine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D001413:Bacillus thuringiensis / Q000737:chemistry / Q000145:classification / Q000472:pathogenicity*; D001517:Coleoptera / Q000187:drug effects / Q000254:growth & development / Q000382:microbiology*; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D010572:Pest Control, Biological; D012703:Serotyping; D013171:Spores, Bacterial; D013400:Sugar Acids / Q000302:isolation & purification / Q000494:pharmacology
D013400:Sugar Acids; C068295:thuringiensin; D000241:Adenosine