title: Pananotin, a potent antifungal protein from roots of the traditional chinese medicinal herb Panax notoginseng.
abstract: The roots of the sanchi ginseng, Panax notoginseng, were extracted with an aqueous buffer. The extract was chromatographed on a CM-cellulose column to remove extraneous unadsorbed proteins. The adsorbed fraction was dialyzed and chromatographed on Affi-gel blue gel. The adsorbed fraction was again collected, dialyzed and applied on a column of Mono S. The second peak was dialyzed and chromatographed on an FPLC-gel filtration Superdex 75 column. An antifungal protein with an N-terminal sequence similar to those of chitinases was isolated from the first peak which had a molecular mass of 35 kDa. The sequence was distinctive in that the third and ninth highly conserved N-terminal residues (C and G) were replaced by H and M, respectively. The protein inhibited mycelial growth in Coprinus comatus, Physalospora piricola, Botrytis cinerea, and Fusarium oxysporum with an IC 50 of 100 nM, 1 microM, 630 nM and 560 nM, respectively. It inhibited cell-free translation with an IC 50 of 630 nM. Its antifungal and translation-inhibitory activities were more potent than those of previously reported antifungal proteins. It inhibited human immunodeficiency virus-1 reverse transcriptase by 35.8 % at 12.6 microM and 24.7 % at 1.26 microM.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
authors.forename: S K
authors.initials: SK
authors.lastname: Lam
authors.forename: T B
authors.initials: TB
authors.lastname: Ng
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology* / Q000627:therapeutic use; D020171:Botrytis / Q000187:drug effects; D003302:Coprinus / Q000187:drug effects; D005658:Fungi / Q000187:drug effects*; D005670:Fusarium / Q000187:drug effects; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D006801:Humans; D006999:Hypocreales / Q000187:drug effects; D020128:Inhibitory Concentration 50; D008516:Medicine, Chinese Traditional; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009181:Mycoses / Q000188:drug therapy; D005894:Panax; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D010940:Plant Proteins / Q000494:pharmacology* / Q000627:therapeutic use; D014176:Protein Biosynthesis / Q000187:drug effects; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D010936:Plant Extracts; D010940:Plant Proteins; D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase