The structure and function of novel proteins of Bacillus anthracis and other spore-forming bacteria: development of novel prophylactic and therapeutic agents.
The overall goal of this review is to summarize the current body of knowledge about the structure and function of major proteins of Bacillus anthracis and/or similar spore-forming organisms. B. anthracis is a key spore-forming biological threat agent, as well as human and animal Gram-positive bacterial pathogen. The structural information described here is limited to approximately the last 5 years. This information is then related to the role of the selected proteins in pathogenesis and in the possible development of novel vaccine and/or other antimicrobial agents against spore-forming organisms, including anthrax, a disease caused by B. anthracis. Among spore-forming bacteria, Bacillus and Clostridium species are the predominant spore-forming bacilli that cause serious diseases. The biochemical properties and mechanism of catalysis of the novel spore germination protease that degrades small, acid-soluble proteins protecting DNA against damage, a cofactor independent phosphoglycerate mutase, NAD+ synthetase, and the three know B. anthracis toxins, protective antigen, lethal factor, and edema factor are described. The studies described in this work review and unify selected information critical for the prevention of microbial diseases such as anthrax. A strategy for the structure-guided development of new prophylactic and therapeutic agents is discussed.
Jedrzejas
Mark J
MJ
Children's Hospital Oakland Research Institute, CA 94609, USA. mjedrzejas@chori.org
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D000881:Anthrax / Q000628:therapy*; D000942:Antigens, Bacterial; D001408:Bacillus anthracis / Q000378:metabolism* / Q000502:physiology*; D001427:Bacterial Toxins / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D003013:Clostridium / Q000378:metabolism; D004249:DNA Damage; D006801:Humans; D013329:Structure-Activity Relationship
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D002352:Carrier Proteins; C030325:anthrax toxin; D000262:Adenylyl Cyclases