Aromatase and prostaglandin inter-relationships in breast adipose tissue: significance for breast cancer development.
The role of prostaglandins in the development of breast cancer is a topic of growing interest. Stimulation of aromatase expression within the breast has been proposed as a mechanism whereby prostaglandins could influence breast cancer growth. In the present study, we show that PGE2 is a powerful stimulator of aromatase expression in human breast adipose stromal cells. Moreover, TNFalpha, which also stimulates aromatase expression in breast adipose stromal cells, acts to increase the secretion of PGE2 by these cells, as well as the expression of COX 2 and PGE synthase, but not that of COX 1. On the other hand, class I cytokines had no effect, either by themselves or in the presence of estradiol. These factors had little influence on secretion of 15-deoxy-delta12, 14-PGJ2, which is inhibitory of aromatase expression by breast adipose stromal cells. These results further substantiate an important role for PGE2 to stimulate estrogen biosynthesis within the local environment of the breast.
Karuppu
Darshini
D
Prince Henry's Institute of Medical Research, Clayton, Victoria, Australia.
Kalus
Alan
A
Simpson
Evan R
ER
Clyne
Colin
C
D000273:Adipose Tissue / Q000187:drug effects* / Q000201:enzymology; D000328:Adult; D001141:Aromatase / Q000096:biosynthesis*; D001940:Breast / Q000187:drug effects* / Q000201:enzymology; D001943:Breast Neoplasms / Q000209:etiology*; D002478:Cells, Cultured; D015232:Dinoprostone / Q000494:pharmacology / Q000502:physiology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D066298:In Vitro Techniques; D008875:Middle Aged; D017154:Stromal Cells / Q000187:drug effects / Q000201:enzymology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D014409:Tumor Necrosis Factor-alpha; D001141:Aromatase; D015232:Dinoprostone