Conservation of avian Z chromosomes as revealed by comparative mapping of the Z-linked aldolase B gene.
A chicken Z-linked BAC probe containing the aldolase B gene was used for fluorescence in-situ hybridization (FISH) mapping in four different avian species. The biotinylated BAC clone showed distinct unique hybridization sites on the structurally different Z chromosomes. This result, together with previous data, lends credence to the notion that, despite undergoing structural rearrangements, the gene content of the avian Z chromosome remained conserved during evolution. Our study also demonstrates the feasibility of using large genomic clones for comparative mapping of Z-linked genes in birds.
Nanda
I
I
Department of Human Genetics, University of Würzburg, Würzburg, Germany.
Schmid
M
M
D000818:Animals; D001717:Birds; D002478:Cells, Cultured; D002642:Chick Embryo; D002874:Chromosome Mapping; D003370:Coturnix / Q000196:embryology / Q000235:genetics*; D004372:Ducks; D004625:Embryo, Nonmammalian / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence
D005634:Fructose-Bisphosphate Aldolase