Characteristics of bioemulsifiers synthesised in crude oil media by Halomonas eurihalina and their effectiveness in the isolation of bacteria able to grow in the presence of hydrocarbons.
Halomonas eurihalina strains F2-7, H28, H96, H212 and H214 were capable of producing large amounts of exopolysaccharides (EPS) in MY medium with added crude oil. The biopolymers showed lower carbohydrate and protein content than those synthesised in control medium without oil. Nevertheless, the percentages of uronic acids, acetyls and sulphates were higher. The emulsifying activity of biopolymers was measured; crude oil was the substrate most efficiently emulsified. Furthermore, all the EPS tested emulsified higher volumes of crude oil than the commercial surfactants used as controls. We have also proved the effectiveness of both Halomonas eurihalina strains and their EPS to select indigenous bacteria able to grow in the presence of polycyclic aromatic hydrocarbons (naphthalene, phenanthrene and pyrene) from waste crude oil. The majority of isolated strains belonged to the genus Bacillus.
Calvo
C
C
Environmental Microbiology Research Group, Institute of Water Research, University of Granada, C/Ramón y Cajal no. 4. Edificio Fray Luis de Granada, 18071 Granada, Spain. ccalvo@platon.ugr.es
Martínez-Checa
F
F
Toledo
F L
FL
Porcel
J
J
Quesada
E
E
D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D001673:Biodegradation, Environmental; D005079:Excipients / Q000378:metabolism / Q000494:pharmacology*; D020632:Halomonas / Q000378:metabolism*; D010578:Petroleum / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000494:pharmacology*
D005079:Excipients; D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D011135:Polysaccharides, Bacterial