title: Microsatellite DNA library for Caiman latirostris.
abstract: New genetic markers were characterized for the broad-snouted caiman (Caiman latirostris) by constructing libraries enriched for microsatellite DNA. Construction and characterization of these libraries are described in the present study. One microsatellite marker was developed from a (ACC-TGG)(n)enriched microsatellite DNA library, and 12 microsatellite markers were developed from a (AC-TG)(n)enriched microsatellite DNA library. These markers were tested in wild-caught animals, and these tests resulted in ten new polymorphic microsatellites for C. latirostris.
authors.affiliation: Centro de Energia Nuclear na Agricultura/USP, Piracicaba, São Paulo, 13400-970, Brasil.
authors.forename: Rodrigo Barban
authors.initials: RB
authors.lastname: Zucoloto
authors.forename: Luciano Martins
authors.initials: LM
authors.lastname: Verdade
authors.forename: Luiz Lehmann
authors.initials: LL
authors.lastname: Coutinho
mesh_terms: D000489:Alligators and Crocodiles / Q000235:genetics*; D000818:Animals; D003001:Cloning, Molecular; D005260:Female; D015723:Gene Library; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*