title: Human cytomegalovirus binding to DC-SIGN is required for dendritic cell infection and target cell trans-infection.
abstract: Cytomegalovirus (CMV) infection is characterized by host immunosuppression and multiorganic involvement. CMV-infected dendritic cells (DC) were recently shown to display reduced immune functions, but their role in virus dissemination is not clear. In this report, we demonstrated that CMV could be captured by DC through binding on DC-SIGN and subsequently transmitted to permissive cells. Moreover, blocking DC-SIGN by specific antibodies inhibited DC infection by primary CMV isolates and expression of DC-SIGN or its homolog DC-SIGNR rendered susceptible cells permissive to CMV infection. We demonstrated that CMV envelope glycoprotein B is a viral ligand for DC-SIGN and DC-SIGNR. These results provide new insights into the molecular interactions contributing to cell infection by CMV and extend DC-SIGN implication in virus propagation.
authors.affiliation: Laboratoire d'immunologie, CNRS UMR 5540, Université Bordeaux 2, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Halary
authors.forename: Ali
authors.initials: A
authors.lastname: Amara
authors.forename: Hugues
authors.initials: H
authors.lastname: Lortat-Jacob
authors.forename: Martin
authors.initials: M
authors.lastname: Messerle
authors.forename: Thierry
authors.initials: T
authors.lastname: Delaunay
authors.forename: Corinne
authors.initials: C
authors.lastname: Houlès
authors.forename: Franck
authors.initials: F
authors.lastname: Fieschi
authors.forename: Fernando
authors.initials: F
authors.lastname: Arenzana-Seisdedos
authors.forename: Jean François
authors.initials: JF
authors.lastname: Moreau
authors.forename: Julie
authors.initials: J
authors.lastname: Déchanet-Merville
mesh_terms: D015815:Cell Adhesion Molecules / Q000276:immunology* / Q000378:metabolism; D003587:Cytomegalovirus / Q000276:immunology* / Q000378:metabolism; D003586:Cytomegalovirus Infections / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D003713:Dendritic Cells / Q000276:immunology / Q000821:virology*; D006367:HeLa Cells; D006801:Humans; D037181:Lectins, C-Type / Q000276:immunology* / Q000378:metabolism; D008024:Ligands; D011956:Receptors, Cell Surface / Q000276:immunology* / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000276:immunology / Q000378:metabolism; D014779:Virus Replication / Q000276:immunology; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D008024:Ligands; D011956:Receptors, Cell Surface; D014759:Viral Envelope Proteins; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C040495:glycoprotein B, Simplexvirus