title: Antioxidant effect of hydroxytyrosol (DPE) and Mn2+ in liver of cadmium-intoxicated rats.
abstract: Liver TBARS formation in cadmium-intoxicated rats was completely reduced by administering a low amount of MnCl(2) (2 mg/kg b.w.) 1 h before intoxication. A similar antioxidant effect was first shown by hydroxytyrosol (2-(3,4-dihydroxyphenyl)ethanol, (DPE), a phenolic compound present in olive oil, given twice to rats (9 mg/kg b.w.) after cadmium administration. The antioxidant properties shown in vivo by both Mn(2+) and DPE were also active in vitro when rat liver microsomes were subjected to lipid peroxidation by cadmium or other prooxidant systems. The increase in liver glutathione concentrations occurring in cadmium-intoxicated rats, was also found, for the first time, 24 h after MnCl(2) administration. Unlike cadmium intoxication, which caused a higher formation of both glutathione and TBARS, Mn(2+) induced glutathione synthesis without any TBARS formation. The same situation was also observed when cadmium plus Mn(2+) or cadmium plus DPE was given to rats. Our data show that: (a). both DPE and low Mn(2+) concentrations may have an antioxidant effect in the livers of cadmium-intoxicated rats and (b). Mn(2+), like cadmium, induces liver glutathione synthesis and this effect is probably independent of TBARS formation.
authors.affiliation: Department of Pharmaco-Biology, University of Bari, Str Prov Per Casamassima, Km 3, 70010 Valenzano,(Ba), Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Casalino
authors.forename: Giovanna
authors.initials: G
authors.lastname: Calzaretti
authors.forename: Cesare
authors.initials: C
authors.lastname: Sblano
authors.forename: Vito
authors.initials: V
authors.lastname: Landriscina
authors.forename: Mario
authors.initials: M
authors.lastname: Felice Tecce
authors.forename: Clemente
authors.initials: C
authors.lastname: Landriscina
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D002104:Cadmium / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008345:Manganese / Q000494:pharmacology* / Q000627:therapeutic use; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D002104:Cadmium; C005975:3,4-dihydroxyphenylethanol; D008345:Manganese; D010626:Phenylethyl Alcohol