Sequence variations in the primer binding regions of the highly polymorphic STR system SE33.
Five cases were found with sequence variation in the primer binding region of the highly polymorphic STR system SE33 used in the German genetic database. This variation can produce homozygote mistyping because of failed primer binding. We calculated a variation rate of 0.0022 (0.0006-0.0056) that could lead to complications in database matching. To avoid errors in individual genetic characterisation for SE33 as described here, it is suggested that two different primer pairs should be used.
Hering
Sandra
S
Institute of Legal Medicine, Technical University of Dresden, Fetscherstrasse 74, 01307 Dresden, Germany. Sandra.Hering@mailbox.tu-dresden.de
Edelmann
Jeanett
J
Dressler
Jan
J
D001483:Base Sequence; D004252:DNA Mutational Analysis / Q000379:methods; D017931:DNA Primers / Q000737:chemistry*; D030561:Databases, Nucleic Acid; D005554:Forensic Medicine; D005858:Germany; D006801:Humans; D008969:Molecular Sequence Data; D010334:Paternity; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences
D017931:DNA Primers