title: New acylated triterpene saponins from Silene fortunei that modulate lymphocyte proliferation.
abstract: Three new acylated triterpene saponins 1-3, with a quillaic acid as aglycon, were isolated from the roots of Silene fortunei together with a known phytoecdysteroid (20-hydroxyecdysone). The compounds were characterized mainly by a combination of 2D NMR techniques, mass spectrometry, and chemical methods. Saponins 1-3, jenisseensosides C and D (4, 5), and 6 (deacylated form of 2/3 and 4/5) were found to stimulate the proliferation of the Jurkat tumor cell lines at low concentration. At high concentration, 2/3 and 4/5 inhibited the proliferation of the cells and suggested the induction of apoptosis.
authors.affiliation: Laboratoire de Pharmacognosie, Unité de Molécules d'Intérêt Biologique, JE 2244, Faculté de Pharmacie, Université de Bourgogne, 7, Bd Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France.
authors.forename: Ghezala
authors.initials: G
authors.lastname: Gaidi
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Miyamoto
authors.forename: Véronique
authors.initials: V
authors.lastname: Laurens
authors.forename: Marie-Aleth
authors.initials: MA
authors.lastname: Lacaille-Dubois
mesh_terms: D000107:Acetylation; D017209:Apoptosis / Q000502:physiology; D006801:Humans; D006868:Hydrolysis; D019169:Jurkat Cells / Q000187:drug effects; D008213:Lymphocyte Activation; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D029751:Silene / Q000737:chemistry*; D013237:Stereoisomerism; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: C471488:3-O-(galactopyranosyl-(1-2)-glucuronopyrnosyl)quillaic acid-28-O-(arabinopyranosyl)-(1-2)-arabinopyranosyl-(1-3)-xylopyranosyl-(1-4)-rhamnopyranosyl-(1-2))-(6-O-acetylglucopyranosyl-(1-3)-4-O-acetylfucopyranoside; C471489:3-O-(galactopyranosyl-(1-2)-glucuronopyrnosyl)quillaic acid-28-O-rhamnopyranosyl-(1-2)-3-O-acetyl-4-p-methoxycinnamoyl fucopyranoside; D012503:Saponins; D014315:Triterpenes; C107206:jenisseensoside C; C107207:jenisseensoside D