Changes in pyridoxal kinase immunoreactivity in the gerbil hippocampus following spontaneous seizure.
To identify the roles of pyridoxal kinase (PLK) in epileptogenesis and the recovery mechanisms in spontaneous seizure, a chronological and comparative analysis of PLK expression in the gerbil hippocampus was conducted. PLK immunoreactivity in a pre-seizure group of seizure sensitive (SS) gerbils was more strongly detected than that in a seizure resistant (SR) group. The density of PLK immunoreactivity in a 30-min postictal group was significantly lower than that of a pre-seizure group. In a 12 h postictal group, PLK immunodensity recovered to pre-seizure level. The over-expression of PLK in the hippocampus of pre-seizure SS gerbils suggests that PLP play an important role in the modulation of GAD activity and GABA reuptake as mediated by membrane transporter via neurons.
Kang
Tae Cheon
TC
Department of Anatomy, College of Medicine, College of Life Science, Hallym University, Kangwon-Do, Chunchon, South Korea. tckang@hallym.ac.kr
Park
Seung Kook
SK
Hwang
In Koo
IK
An
Sung Jin
SJ
Bahn
Jae Hoon
JH
Kim
Dae Won
DW
Choi
Soo Young
SY
Kwon
Oh Shin
OS
Baek
Nam In
NI
Lee
Hyeon Yong
HY
Won
Moo Ho
MH
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D018891:Dentate Gyrus / Q000201:enzymology / Q000503:physiopathology; D015536:Down-Regulation / Q000502:physiology; D004827:Epilepsy / Q000201:enzymology* / Q000503:physiopathology; D005455:Fluorescent Antibody Technique; D050485:GABA Plasma Membrane Transport Proteins; D005849:Gerbillinae; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D006624:Hippocampus / Q000201:enzymology* / Q000503:physiopathology; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins; D009433:Neural Inhibition / Q000502:physiology; D009474:Neurons / Q000201:enzymology*; D027361:Organic Anion Transporters; D011731:Pyridoxal Kinase / Q000378:metabolism*; D011930:Reaction Time / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D002352:Carrier Proteins; D050485:GABA Plasma Membrane Transport Proteins; D007527:Isoenzymes; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D027361:Organic Anion Transporters; D005680:gamma-Aminobutyric Acid; D011731:Pyridoxal Kinase; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1; C401141:glutamate decarboxylase 2