Anti-nociceptive effects of diazepam binding inhibitor in the central nervous system of rats.
The present study investigated the effect of diazepam binding inhibitor (DBI) on nociception in the central nervous system of rats. There were dose-dependent increases in hindpaw withdrawal latency (HWL) to noxious thermal and mechanical stimulation after intrathecal injection of 1, 5 or 10 microg of DBI in rats, indicating a DBI-induced anti-nociceptive effect at the spinal levels of rats. Furthermore, it was found that there were no significant influences of intrathecal co-administration of gamma-aminobutyric acid (GABA) on the intrathecal DBI-induced increases in HWLs of rats. Intracerebroventricular administration of 1, 10 or 20 microg of DBI also induced dose-dependent increases in HWL to thermal and mechanical stimulation in rats, suggesting an anti-nociceptive effect of DBI in the brain. Moreover, there were no significant influences of intracerebroventricular co-administration of 2 microg of GABA on the intracerebroventricular DBI-induced increases in HWL of rats. The results of the present study demonstrated that DBI played anti-nociceptive effects in the central nervous system of rats.
Wang
Wei
W
Department of Physiology, College of Life Science, National Laboratory of Biomembrane and Membrane Biotechnology and Center for Brain and Cognitive Science, Peking University, Beijing 100871, PR China.
Wu
Dong-Chuan
DC
Chen
Ya-Hui
YH
He
Wei
W
Yu
Long-Chuan
LC
D000818:Animals; D002490:Central Nervous System / Q000187:drug effects*; D026261:Diazepam Binding Inhibitor / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007276:Injections, Intraventricular; D007278:Injections, Spinal; D008297:Male; D010146:Pain / Q000188:drug therapy*; D010147:Pain Measurement; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time; D005680:gamma-Aminobutyric Acid / Q000008:administration & dosage / Q000502:physiology
D026261:Diazepam Binding Inhibitor; D005680:gamma-Aminobutyric Acid