[Immunoblotting observation on changes of myosin degradation metabolism in toad gastrocnemius muscle electric stimulation (correction of stmulation) under simulated exercise load].
Objective. To probe into the optimum exercise load in sports training. Method. Myosin heavy chain degradation fragments produced by different-intensity electric stimulation were identified in toad gastrocnemius muscle. The fragments were identified by electrophoresis of unfractionated extracts of toad gastrocnemius muscle on sodium dodecyl sulfate/polyacrylamide gels followed by immunoblotting on nitrocellulose sheets. Polyclonal antibody directed against the entire myosin were used to characterize the fragments. Result. Toad gastrocnemius muscle protein degradation increased under high-intensity electric stimulation; two kinds of Myosin degradation fragments whose molecular weight were 43KD,38KD respectively were produced by double high-intensity electric stimulation; the molecular weight 43KD immunoreactive myosin fragments produced by high-intensity electric stimulation disappeared under low-intensity electric stimulation. Conclusion. Continuous high-intensity exercise load would make muscle contractile protein further degradate while low-intensity exercise load would enhance the recover of contraction and function of the muscle.
Ni
C Z
CZ
Institute of Space Medico-Engineering, Beijing, China.
D000818:Animals; D002025:Bufonidae; D003285:Contractile Proteins / Q000378:metabolism; D004558:Electric Stimulation; D015151:Immunoblotting; D008970:Molecular Weight; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D018995:Myosin Heavy Chains / Q000378:metabolism*; D015879:Myosin Subfragments / Q000378:metabolism*; D010805:Physical Conditioning, Animal / Q000502:physiology
D003285:Contractile Proteins; D009124:Muscle Proteins; D015879:Myosin Subfragments; D018995:Myosin Heavy Chains