title: The lack of relationship between serum content of MBL, sCD14, anti-PPD and anti-Hsp65 IgG and ingestion of Mycobacterium bovis BCG bacilli by phagocytes.
abstract: Prophylactic vaccination against tuberculosis (TB) with a live attenuated strain of Mycobacterium bovis bacille Calmette-Guérin (BCG) has been used worldwide. However, TB remains one of the most significant diseases of humans and animals. Better understanding of the mechanisms of human immunity to mycobacteria is essential for the development of new vaccines and the estimation of their efficacy. In this study we determined the levels of known humoral mediators of mycobacterial phagocytosis, i.e. mannose-binding lectin (MBL), soluble CD14 (sCD14), antibodies of the immunoglobulin G (IgG) class against mycobacterial purified protein derivative (PPD), and mycobacterial Hsp65 antigen, in the sera from healthy young volunteers vaccinated with BCG and presenting positive and negative Mantoux responses to PPD. Then we asked the question as to whether macrophages and polymorphonuclear leukocytes (PMNs) from the individuals with positive tuberculin test (TT(+)) and negative tuberculin test (TT(-)) differed in their ability to ingest mycobacteria. We also looked for a relationship between the intensity of mycobacterial ingestion by phagocytes in a medium of autologous sera containing different concentrations of MBL, sCD14 and anti-mycobacterial IgG. We found no significant differences between the investigated parameters for TT(+) and TT(-) volunteers. Our result suggest that the ability of macrophages and PMNs to ingest mycobacteria depends on an individual, intrinsic capacity of the phagocytes.
authors.affiliation: Department of Immunology and Biology of Infections, University of Lódź, Lódź, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Paziak-Domańska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Bonar
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kowalewicz-Kulbat
authors.forename: Magdalena
authors.initials: M
authors.lastname: Klink
authors.forename: Michał
authors.initials: M
authors.lastname: Kowalski
authors.forename: Jari
authors.initials: J
authors.lastname: Karhukorpi
authors.forename: Ritta
authors.initials: R
authors.lastname: Karttunen
authors.forename: Magdalena
authors.initials: M
authors.lastname: Jurkiewicz
authors.forename: Barbara
authors.initials: B
authors.lastname: Rózalska
authors.forename: Wiesława
authors.initials: W
authors.lastname: Rudnicka
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001500:BCG Vaccine / Q000276:immunology / Q000494:pharmacology; D001426:Bacterial Proteins; D018834:Chaperonin 60; D018833:Chaperonins / Q000276:immunology; D006801:Humans; D006968:Hypersensitivity, Delayed; D007074:Immunoglobulin G / Q000097:blood; D066298:In Vitro Techniques; D018950:Lipopolysaccharide Receptors / Q000097:blood*; D037601:Mannose-Binding Lectin / Q000097:blood*; D009163:Mycobacterium bovis / Q000276:immunology*; D010586:Phagocytes / Q000276:immunology* / Q000382:microbiology; D010587:Phagocytosis; D012995:Solubility; D014373:Tuberculin / Q000276:immunology; D014374:Tuberculin Test; D014397:Tuberculosis, Pulmonary / Q000276:immunology / Q000517:prevention & control
chemical_list: D000907:Antibodies, Bacterial; D001500:BCG Vaccine; D001426:Bacterial Proteins; D018834:Chaperonin 60; D007074:Immunoglobulin G; D018950:Lipopolysaccharide Receptors; D037601:Mannose-Binding Lectin; D014373:Tuberculin; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins