The utility of green fluorescent protein in transgenic plants.
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has proven to be a powerful tool in plant genetic transformation studies. This paper reviews the history and the progression of the expression of GFP variants in transgenic plants. The distinguishing features of the most useful GFPs, such as those including the S65T chromophore mutation and those with dual excitation peaks, are discussed. The review also focuses on the utility of GFP as a visual selectable marker in aiding the plant transformation process; GFP has been more important in monocot transformation compared with dicot transformation. Finally, the potential utility of new fluorescent proteins is speculated upon.
Stewart
C N
CN
Department of Biology, University of North Carolina-Greensboro, NC 27402-6174, USA. nstewart@uncg.edu.
D000818:Animals; D003063:Cnidaria; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins