title: Amphotericin B lipid complex exerts additive antifungal activity in combination with polymorphonuclear leucocytes against Scedosporium prolificans and Scedosporium apiospermum.
abstract: Scedosporium prolificans and Scedosporium apiospermum are resistant to most antifungal agents and cause refractory pulmonary and disseminated infections. The combined effects of deoxycholate amphotericin B, amphotericin B lipid complex and liposomal amphotericin B with human polymorphonuclear leucocytes (PMNs) in damaging hyphae of these fungi were evaluated by XTT assay. Amphotericin B lipid complex displayed a significant additive effect with PMNs against both Scedosporium species (22% for S. prolificans and 81% for S. apiospermum; P < 0.04). None of the formulations adversely affected the PMN antifungal activity. These findings may be important in designing better strategies for management of infections due to these organisms.
authors.affiliation: Third Paediatric Department, Aristotle University, Hippokration Hospital, Thessaloniki GR-54642, Greece.
authors.forename: Cristina
authors.initials: C
authors.lastname: Gil-Lamaignere
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Roilides
authors.forename: Avgi
authors.initials: A
authors.lastname: Maloukou
authors.forename: Irene
authors.initials: I
authors.lastname: Georgopoulou
authors.forename: George
authors.initials: G
authors.lastname: Petrikkos
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Walsh
mesh_terms: D000328:Adult; D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D004338:Drug Combinations; D004359:Drug Therapy, Combination; D006801:Humans; D009504:Neutrophils / Q000382:microbiology*; D010713:Phosphatidylcholines / Q000494:pharmacology*; D010715:Phosphatidylglycerols / Q000494:pharmacology*; D021681:Scedosporium / Q000187:drug effects* / Q000302:isolation & purification; D018709:Statistics, Nonparametric
chemical_list: D000935:Antifungal Agents; D004338:Drug Combinations; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; C068538:liposomal amphotericin B; D000666:Amphotericin B