Construction and characterization of insertion/deletion mutations of the tutF, tutD, and tutG genes of Thauera aromatica strain T1.
Thauera aromatica T1 was isolated for its ability to use toluene as a sole carbon source under denitrifying conditions. A genetic approach was used to examine the roles of the tutF, tutD, and tutG gene products (part of a single operon) in the metabolism of toluene. The genes were individually deleted from the chromosome and each resulting mutant strain was unable to metabolize toluene. Plasmids carrying individual in-frame gene deletions failed to complement the corresponding chromosomal deletions but did complement chromosomal deletions downstream of the in-frame deletion. Hence, the tutF, tutD, and tutG genes are each essential for toluene metabolism in T. aromatica T1.
Coschigano
Peter W
PW
Department of Biomedical Sciences and Program in Molecular and Cellular Biology, College of Osteopathic Medicine, Ohio University, Athens, OH 45701-2979, USA. coschiga@ohiou.edu
D003001:Cloning, Molecular; D017931:DNA Primers; D017353:Gene Deletion; D005798:Genes, Bacterial; D005816:Genetic Complementation Test / Q000379:methods; D008957:Models, Genetic; D016254:Mutagenesis, Insertional; D009154:Mutation; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D020628:Thauera / Q000235:genetics* / Q000378:metabolism*; D014050:Toluene / Q000032:analysis / Q000378:metabolism*
D017931:DNA Primers; D014050:Toluene