Production of anti-virus, viroid plants by genetic manipulations.
Many pathogenic plant viruses are RNA viruses, which initiate production of double-stranded RNA intermediates when they replicate in host plant cells. Introduction of double-stranded RNA-specific ribonucleases such as the Schizosaccharomyces pombe derived pac I protein and animal cell derived interferon-induced 2',5'-oligoadenylate synthetase (2-5 Aase)/ribonuclease L (RNase L) system into various plants may make plants resistant to various pathogenic viruses and viroids. We have demonstrated that pac I and 2-5 Aase/RNase L transgenic tobacco plants are resistant to various viruses including tobacco mosaic virus, cucumber mosaic virus and potato virus Y. In addition, pac I transgenic potato plants are resistant to potato spindle tuber viroid. Using Agrobacterium-mediated transformation, we have established a transformation system for chrysanthemum plants and have recently developed pac I transgenic chrysanthemum (Dendranthema grandiflora cv Reagan) resistant to chrysanthemum stunt viroid and have grown them in isolated fields for an evaluation of their effects.
Ishida
Isao
I
Pharmaceutical Division, Kirin Brewery Co Ltd, Tokyo 104-8288, Japan. i-ishida@kirin.co.jp
Tukahara
Masayoshi
M
Yoshioka
Masaharu
M
Ogawa
Toshiya
T
Kakitani
Mokoto
M
Toguri
Toshihiro
T
D031202:Chrysanthemum / Q000235:genetics / Q000821:virology; D017799:Cucumovirus / Q000235:genetics / Q000254:growth & development; D007113:Immunity, Innate / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D010942:Plant Viruses / Q000235:genetics* / Q000254:growth & development; D010944:Plants / Q000235:genetics* / Q000821:virology; D030821:Plants, Genetically Modified; D017800:Potyvirus / Q000235:genetics / Q000254:growth & development; D012328:RNA Viruses / Q000235:genetics* / Q000254:growth & development; D011198:Solanum tuberosum / Q000235:genetics / Q000821:virology; D014026:Nicotiana / Q000235:genetics / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000235:genetics / Q000254:growth & development; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics; D014772:Viroids / Q000235:genetics* / Q000254:growth & development
D054334:Viral Regulatory and Accessory Proteins; C073671:SPP1 pac region protein, Bacteriophage SPP1