Self-assembled ternary complex of cationic dendrimer, cucurbituril, and DNA: noncovalent strategy in developing a gene delivery carrier.
A ternary complex of PPI-DAB dendrimer [(1,4-diaminobutane); Gen = N; dendri-poly(propyleneimine); -[NHC(=O)CH(2)NH(2)(+)(CH(2))(4)NH(3)(+)](z)()], DNA, and cucurbituril (CB) was evaluated as an example of a totally self-assembled gene delivery carrier. The complex was formed in a noncovalent way in which DNA interacts with PPI-DAB electrostatistically and CB with PPI-DAB through multiple noncovalent interactions. Dynamic light scattering data indicated that the diameter and size distributions of the complexes were dependent upon the sequence of mixing of each component with unimodal distribution ranging from 150.8 to 210.2 nm under favorable conditions. Fluorescence studies showed the quantitative binding of CB to PPI-DAB after ternary complex formation. The complex was able to transfect mammalian cells with high efficiency and the cytotoxicity of the PPI-DAB/CB complex was relatively low.
Lim
Yong-Beom
YB
School of Chemistry & Molecular Engineering, Seoul National University, Seoul 151-742, Korea.
Kim
Taeil
T
Lee
Jae Wook
JW
Kim
Seon-Mi
SM
Kim
Hee-Joon
HJ
Kim
Kimoon
K
Park
Jong-Sang
JS
D000818:Animals; D001952:Bridged-Ring Compounds / Q000009:adverse effects / Q000737:chemistry*; D002412:Cations / Q000009:adverse effects / Q000737:chemistry*; D002522:Chlorocebus aethiops; D004247:DNA / Q000009:adverse effects / Q000737:chemistry*; D004337:Drug Carriers / Q000009:adverse effects / Q000737:chemistry*; D007093:Imidazoles / Q000009:adverse effects / Q000737:chemistry*; D008027:Light; D011108:Polymers / Q000009:adverse effects / Q000737:chemistry*; D011700:Putrescine / Q000009:adverse effects / Q000737:chemistry*; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D055672:Static Electricity; D014162:Transfection / Q000379:methods*; D014709:Vero Cells; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils
D001952:Bridged-Ring Compounds; D002412:Cations; D004247:DNA; D004337:Drug Carriers; D007093:Imidazoles; D011108:Polymers; D011700:Putrescine; C454805:cucurbit(6)uril; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils