[Physical mapping of DNA of cyanophage LPP-3].
Restrictases fit for the purposes of physical mapping of cyanophage LPP-3 DNA have been selected as a result of the restriction analysis. The use of the methods of mutual hydrolysis, restriction of the fragment isolated from gel and terminal labeling allowed formation a physical map of LPP-3 cyanophage DNA with the complete scheme of allocation of 14 sites for 8 restrictases: Alw44I, Bsp1191, BsuRI, Eco147I, EheI, NcoI, Kpn2I and PvuI as well as the position of certain sites for restrictases HindIII, KpnI and Sau3A.
Syrchin
S A
SA
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Mendzhul
M I
MI
D001435:Bacteriophages / Q000235:genetics*; D000458:Cyanobacteria / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics*; D015183:Restriction Mapping / Q000379:methods
D004279:DNA, Viral