New automated method for determining postantifungal effect of amphotericin B against Candida species: effects of concentration, exposure time, and area under the curve.
The postantifungal effect (PAFE) of amphotericin B was determined with a BacT/Alert automated system, which is based on the colorimetric detection of CO(2). The levels of accuracy and precision of the automated method were high. Longer PAFEs were obtained for Candida albicans (P < 0.001), C. glabrata (P < 0.01), and C. krusei (P < 0.01) at increasing amphotericin B concentrations and exposure times.
Chryssanthou
Erja
E
Department of Clinical Microbiology, Karolinska Hospital, Stockholm, Sweden. Erja.Chryssanthou@ks.se
Cars
Otto
O
Sjölin
Jan
J
D000666:Amphotericin B / Q000493:pharmacokinetics / Q000494:pharmacology*; D019540:Area Under Curve; D002175:Candida / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D008826:Microbial Sensitivity Tests / Q000379:methods; D013045:Species Specificity; D013997:Time Factors
D000666:Amphotericin B