Effect of different growth factors on human cultured osteoblast-like cells.
OBJECTIVE
Proliferation and differentiation of osteoblast-like cells are regulated by complex interactions among systemic hormones, cytokines, and local growth factors. The success of oral rehabilitation using biomaterial implants depends on the growth of osteoblasts and their adhesivity to the surface of the implant. The study aimed to investigate the effect of different growth factors on the proliferation and adhesivity of human osteoblast-like cells in vitro.


METHODS
The effects of transforming growth factor beta1 (TGFbeta1), fibroblast growth factor (FGF), and interleukin-2 (IL-2) on the growth and adhesivity of human osteoblast-like cells in monolayer culture were studied. Their growth was measured by (3)H-thymidine incorporation and their adhesivity by flow cytometry.


RESULTS
Incorporation of (3)H-thymidine was increased by all growth factors. The effect did not appear to be dose dependent. No synergic effect was found between TGFbeta1 and FGF. Treatment with TGFbeta1 or FGF increased cell adhesivity by shortening the time needed for cell adhesion to the culture dish surface and hydroxyapatite-coated implants. IL-2 did not modify cell adhesivity.


CONCLUSIONS
Growth factors can modulate cell proliferation and adhesivity, which may help to increase the success of implantation therapy.
Reyes-Botella
Candela
C
Department of Stomatology, Physiology Section, Institute of Neuroscience, University of Granada, Madrid, Spain.
Vallecillo-Capilla
Manuel F
MF
Ruiz
Concepción
C
D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D000918:Antibody Specificity; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry; D005260:Female; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D007376:Interleukin-2 / Q000494:pharmacology*; D008297:Male; D015260:Neprilysin / Q000096:biosynthesis; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1; D014316:Tritium
D020099:Coated Materials, Biocompatible; D018960:Hyaluronan Receptors; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D014316:Tritium; D015675:Osteocalcin; D005346:Fibroblast Growth Factors; D017886:Durapatite; D000469:Alkaline Phosphatase; D015260:Neprilysin; D013936:Thymidine