Autoantibody to DNA binding protein B as a novel serologic marker in systemic sclerosis.
Systemic sclerosis is a systemic disease that is characterized by tissue fibrosis, small-vessel vasculopathy, and an autoimmune response associated with autoantibodies. We performed serological analysis of cDNA expression library (SEREX) to identify autoantibodies associated with systemic sclerosis. We identified 4 clones that react with sera of patients with SSc but not with those of healthy donors. These clones are phosphoglycerate mutase, centromere autoantigen C, U1 small nuclear ribonucleoprotein, and DNA binding protein B (dbpB). We chose to study autoantibody to DNA binding protein B. Immunoreactivity against recombinant dbpB was detected in 40.5% (15/37) of patients with SSc, 14.6% (6/41) of patents with systemic lupus erythematosus, 6.7% (1/15) of patients with rheumatoid arthritis, 0% (0/12) of patients with Sjogren syndrome, and 5.9% (1/17) of patients with polymyositis/dermatomyositis. The frequency of anti-dbpB was significantly higher in the SSc patients (15/37, 40.5%) compared to the healthy controls (3/41, 7.3%, p=0.0005 by chi(2) test). Eleven patients (11/20, 55%) with the diffuse cutaneous type of SSc had anti-dbpB and 4 patients (4/17, 23.5%) with the limited cutaneous type had anti-dbpB. The presence of anti-dbpB was significantly associated with the diffuse cutaneous type (p=0.00003 by chi(2) test). This is the first report to suggest that autoantibody to dbpB can be used as a serologic marker of systemic sclerosis.
Jeoung
Doo-il
DI
Cancer Genomics Division, In2Gen Company, 6th Floor, Cancer Research Center, Seoul National University College of Medicine, 228, Yongon-dong, Chongno-gu, Seoul 110-799, Republic of Korea.
Bong Lee
Eun
E
Lee
Seongeun
S
Lim
Yoon
Y
Lee
Dae-Yeon
DY
Kim
Jongwan
J
Kim
Hae-Yeong
HY
Wook Song
Yeong
Y
D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers; D022762:CCAAT-Enhancer-Binding Proteins / Q000276:immunology* / Q000378:metabolism; D004268:DNA-Binding Proteins; D005260:Female; D015723:Gene Library; D006801:Humans; D008297:Male; D008875:Middle Aged; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D012595:Scleroderma, Systemic / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D012698:Serologic Tests; D014157:Transcription Factors / Q000276:immunology / Q000378:metabolism; D051840:Y-Box-Binding Protein 1
D001323:Autoantibodies; D015415:Biomarkers; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human