ST2 protein induced by inflammatory stimuli can modulate acute lung inflammation.
We have investigated gene and protein expression of ST2/ST2L in a murine alveolar macrophage (AM) cell line, MH-S, reacting to inflammatory stimuli in vitro and in the lung tissue of an acute lung injury model in vivo. We have also analyzed the effect of soluble ST2 protein on inflammatory response of MH-S cells. Lipopolysaccharide (LPS) and proinflammatory cytokines such as IL-1beta, IL-6, and TNF-alpha induced ST2 mRNA expression in MH-S cells. In an acute lung injury model, protein and mRNA expression levels of ST2 increased to the maximal level at 24-72h after the LPS challenge. Furthermore, pretreatment with ST2 protein significantly reduced the protein production and gene expression of IL-1alpha, IL-6, and TNF-alpha in LPS-stimulated MH-S cells in vitro. These results suggest that increases in endogenous ST2 protein in AM, which is induced by inflammatory stimuli, such as LPS and proinflammatory cytokines, may modulate acute lung inflammation.
Oshikawa
Katsuhisa
K
Division of Pulmonary Medicine, Department of Medicine, Jichi Medical School, 3311 Minamikawachi, Kawachi-gun, 329-0498, Tochigi, Japan. oshikatu@jichi.ac.jp
Yanagisawa
Ken
K
Tominaga
Shin ichi
Si
Sugiyama
Yukihiko
Y
D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D007249:Inflammation; D007375:Interleukin-1 / Q000378:metabolism; D000072179:Interleukin-1 Receptor-Like 1 Protein; D015850:Interleukin-6 / Q000378:metabolism; D007700:Kinetics; D008070:Lipopolysaccharides / Q000378:metabolism; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D055370:Lung Injury; D008264:Macrophages / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000378:metabolism; D018123:Receptors, Interleukin; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; C486189:Il1rl1 protein, mouse; D007375:Interleukin-1; D000072179:Interleukin-1 Receptor-Like 1 Protein; D015850:Interleukin-6; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D014409:Tumor Necrosis Factor-alpha