title: Colony multiplex PCR assay for identification and differentiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus.
abstract: A multiplex PCR assay was used to simultaneously detect genes from the five major clinically relevant Campylobacter spp. Those genes selected were hipO and 23S rRNA from Campylobacter jejuni; glyA from each of C. coli, C. lari, and C. upsaliensis; and sapB2 from C. fetus subsp. fetus. The assay was evaluated with 137 clinical and environmental isolates and was found to be rapid and easy to perform and had a high sensitivity and specificity for characterizing isolates, even in mixed cultures.
authors.affiliation: National Laboratory for Enteric Pathogens, National Microbiology Laboratory, Winnipeg, Manitoba, Canada. gehua_Wang@hc-sc.gc.ca
authors.forename: Gehua
authors.initials: G
authors.lastname: Wang
authors.forename: Clifford G
authors.initials: CG
authors.lastname: Clark
authors.forename: Tracy M
authors.initials: TM
authors.lastname: Taylor
authors.forename: Chad
authors.initials: C
authors.lastname: Pucknell
authors.forename: Connie
authors.initials: C
authors.lastname: Barton
authors.forename: Lawrence
authors.initials: L
authors.lastname: Price
authors.forename: David L
authors.initials: DL
authors.lastname: Woodward
authors.forename: Frank G
authors.initials: FG
authors.lastname: Rodgers
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002167:Campylobacter / Q000145:classification* / Q000235:genetics; D002169:Campylobacter Infections / Q000382:microbiology*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial