Effect of bombesin on gut mucosal impairment after severe burn.
Severe cutaneous bum alters gut epithelial homeostasis. In previous studies, treatment with bombesin decreased mucosal atrophy and improved maintenance of gut mucosal integrity after severe burn. Our current hypothesis is that bombesin reduces burn-induced gut impairment by decreasing gut epithelial cell death. Fifty-four adult male Fisher-344 rats were randomly assigned to three groups: control, sham burn (I), burn (II), and burn + bombesin (III). Animals in groups II and III received a 60% total body surface area full thickness scald burn, and the treatment group (III) received bombesin subcutaneously (10 microg/kg, every 8 h) beginning immediately before the experiment. The proximal small bowel was harvested at 12 and 72 h after burn with measurement of wet and dry weight, mucosal weight, and protein content, and a 1-cm length of proximal end was excised and fixed in fomalin for histological and immunohistochemical observation. Data are expressed as means +/- SEM. Statistical analysis was by done by analysis of variance (significance at P < 0.05). Bombesin treatment attenuated mucosal atrophy demonstrated by restoration of the mucosal weight, mucosal protein content, and maintenance of mucosal height and total mucosal epithelial cell count. Gut epithelial cell apoptosis was, at least in part, inhibited by bombesin compared with a significant increase of gut cell apoptosis at 12 h after burn. Gut epithelial proliferation was not affected. Bombesin diminished burn-induced gut mucosal atrophy and gut epithelial cell apoptosis, suggesting that bombesin treatment may play an important role in the recovery of gut impairment after severe burn.
Wu
Xiao-Wu
XW
Shriner's Burns Hospital and Department of Surgery, University of Texas Medical Branch, Galveston, Texas 77550, USA.
Spies
Marcus
M
Chappell
Vicky L
VL
Herndon
David N
DN
Thompson
James C
JC
Wolf
Steven E
SE
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001839:Bombesin / Q000494:pharmacology* / Q000627:therapeutic use; D002056:Burns / Q000150:complications* / Q000188:drug therapy* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology* / Q000503:physiopathology; D007421:Intestine, Small / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D008297:Male; D009929:Organ Size; D051381:Rats; D011916:Rats, Inbred F344; D013997:Time Factors; D015599:Trauma Severity Indices
D001839:Bombesin