Reverse genetic analysis of the Caenorhabditis elegans 26S proteasome subunits by RNA interference.
Reverse genetic analysis was performed on the Caenorhabditis elegans 26S proteasome subunit genes by double-stranded RNA-mediated interference (RNAi). Embryonic and post-embryonic lethality was caused by interference of all of the eight tested 20S core subunits and all of the 19S regulatory particle subunits except for Ce-Rpn9, Ce-Rpn10, and Ce-Rpn12, where RNAi caused no abnormality. However, synthetic suppression of Ce-Rpn10 and Ce-Rpn12 was lethal, whereas neither the combination of Ce-Rpn9 with Ce-Rpn10 nor with Ce-Rpn12 resulted in abnormalities in RNAi. These results indicate that the 26S proteasome is indispensable for embryogenesis and post-embryonic development, although Ce-Rpn9, Ce-Rpn10, and Ce-Rpn12 are not essential, at least under the conditions used. Ce-Rpn10 and Ce-Rpn12 are considered to compensate for the suppression of each other.
Takahashi
Maiko
M
School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
Iwasaki
Hideki
H
Inoue
Hideshi
H
Takahashi
Kenji
K
D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology / Q000254:growth & development; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000502:physiology; D023281:Genomics / Q000379:methods; D010447:Peptide Hydrolases / Q000235:genetics* / Q000502:physiology; D046988:Proteasome Endopeptidase Complex; D021122:Protein Subunits / Q000235:genetics / Q000502:physiology; D034622:RNA Interference; D015996:Survival Rate
D029742:Caenorhabditis elegans Proteins; D021122:Protein Subunits; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease