Induction of the intrinsic apoptotic pathway in cells infected with cytopathic bovine virus diarrhoea virus.
Cytopathic bovine viral diarrhoea virus (cp BVDV) induces apoptosis in bovine cell cultures. This also seems to be a prominent feature in the pathogenesis of mucosal disease. To gain an insight into the molecular pathways of the cell alterations, the involvement of different members of the apoptotic cascade was analyzed. It was shown that inhibition of the mitochondrial permeability transition pore significantly delayed the cytopathic effect without affecting virus replication. Moreover, the membrane potential (deltapsi(m)) was affected, and translocation of cytochrome c to the cytosol, overexpression of apoptotic protease-activating factor 1 and a significant increase of caspase-9 activity were demonstrated, indicating that the apoptosome is formed. We conclude that at least in vitro, infection of cells with cp BVDV leads to the activation of the intrinsic pathway of apoptosis.
Grummer
B
B
Institute of Virology, School of Veterinary Medicine, Buenteweg 17, 30559, Hannover, Germany.
Bendfeldt
S
S
Wagner
B
B
Greiser-Wilke
I
I
D000818:Animals; D017209:Apoptosis; D053477:Apoptotic Protease-Activating Factor 1; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000821:virology; D053453:Caspase 9; D020169:Caspases / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D003574:Cytochrome c Group / Q000378:metabolism; D003588:Cytopathogenic Effect, Viral; D001908:Diarrhea Viruses, Bovine Viral / Q000472:pathogenicity*; D007473:Ion Channels; D008564:Membrane Potentials; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D011506:Proteins / Q000378:metabolism
D053477:Apoptotic Protease-Activating Factor 1; D003574:Cytochrome c Group; D007473:Ion Channels; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D011506:Proteins; D053453:Caspase 9; D020169:Caspases