Promoters P3, Pa/Pb, P4, and P5 upstream from bla(TEM) genes and their relationship to beta-lactam resistance.
Using an isogenic system, we have determined the impact that the four promoters known to control bla(TEM) gene expression have on beta-lactamase activity. For both TEM-1 and TEM-30, this activity gradually increased in relation to the presence of promoters P3, Pa/Pb, and P4 upstream of the corresponding gene. Promoter P5, only found upstream of the bla(TEM-1B) gene, was related to the highest expression of this gene.
Lartigue
Marie Frédérique
MF
Service de Microbiologie-Hygiène, Hôpital Ambroise Paré Assistance Publique-Hôpitaux de Paris, Boulogne, France. marie-helene.nicolas-chanoine@apr.ap-hop-paris.fr
Leflon-Guibout
Véronique
V
Poirel
Laurent
L
Nordmann
Patrice
P
Nicolas-Chanoine
Marie-Hélène
MH
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D011401:Promoter Regions, Genetic / Q000235:genetics*; D001618:beta-Lactamases / Q000187:drug effects / Q000235:genetics*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases