Use of control analysis to study the regulation of plant lipid biosynthesis.
Control analysis is a powerful method to quantify the regulation of metabolic pathways. We have applied it to lipid biosynthesis for the first time by using model tissue culture systems from the important oil crops, olive ( Olea europaea L.) and oil palm ( Elaeis guineensis Jacq.). By the use of top-down control analysis, fatty acid biosynthesis has been shown to exert more control than lipid assembly under different experimental conditions. However, both parts of the lipid biosynthetic pathway are important, so that attempts to alter oil yield by manipulating the activity of a single enzyme step are very unlikely to produce significant increases.
Ramli
U S
US
Malaysian Palm Oil Board, P.O. Box 10620, 50700 Kuala Lumpur, Malaysia.
Baker
D S
DS
Quant
P A
PA
Harwood
J L
JL
D001671:Biochemistry / Q000379:methods; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D050356:Lipid Metabolism; D008055:Lipids / Q000096:biosynthesis*; D013696:Temperature
D005227:Fatty Acids; D008055:Lipids