title: Alkane hydroxylase homologues in Gram-positive strains.
abstract: We isolated Gram-positive alkane-degraders from soil and a tricking-bed reactor, and show using polymerase chain reaction (PCR) with degenerate alkane hydroxylase primers and Southern blots that most Rhodococcus isolates contain three to five quite divergent homologues of the Pseudomonas putida GPo1 alkB gene. Two Mycobacterium isolates each contain one homologue, however there is no evidence for the presence of alkB homologues in the remaining strains.
authors.affiliation: Institute of Biotechnology, Swiss Federal Institute of Technology (ETH), ETH-Hönggerberg, CH-8093 Zürich, Switzerland.
authors.forename: Jan B
authors.initials: JB
authors.lastname: van Beilen
authors.forename: Theo H M
authors.initials: TH
authors.lastname: Smits
authors.forename: Lyle G
authors.initials: LG
authors.lastname: Whyte
authors.forename: Susanne
authors.initials: S
authors.lastname: Schorcht
authors.forename: Martina
authors.initials: M
authors.lastname: Röthlisberger
authors.forename: Thorsten
authors.initials: T
authors.lastname: Plaggemeier
authors.forename: Karl-Heinrich
authors.initials: KH
authors.lastname: Engesser
authors.forename: Bernard
authors.initials: B
authors.lastname: Witholt
mesh_terms: D000473:Alkanes / Q000378:metabolism; D015139:Blotting, Southern; D042926:Cytochrome P-450 CYP4A; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D006094:Gram-Positive Bacteria / Q000145:classification / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology
chemical_list: D000473:Alkanes; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D042926:Cytochrome P-450 CYP4A