title: Photoinduced cleavage by a rhodium complex at G.U mismatches and exposed guanines in large and small RNAs.
abstract: Photoinduced cleavage reactions by the rhodium complex tris(4,7-diphenyl-1,10-phenanthroline)rhodium(III) [Rh(DIP)(3)(3+)] with three RNA hairpins, r(GGGGU UCGCUC CACCA) (16 nucleotide, tetraloop(Ala2)), r(GGGGCUAUAGCUCUAGCUC CACCA) (24 nucleotide, microhelix(Ala)), and r(GGCGGUUAGAUAUCGCC) (17 nucleotide, 790 loop), and full-length (1542 nucleotide) 16S rRNA from Escherichia coli were investigated. The cleavage reactions were monitored by gel electrophoresis and the sites of cleavage by Rh(DIP)(3)(3+) were determined by comparisons with chemical or enzymatic sequencing reactions. In general, RNA backbone scission by the metal complex was induced at G.U mismatches and at exposed G residues. The cleavage activity was observed on the three small RNA hairpins as well as on the isolated 1542-nucleotide ribosomal RNA.
authors.affiliation: Department of Chemistry, Wayne State University, Detroit, MI 48202, USA. csc@chem.wayne.edu
authors.forename: Christine S
authors.initials: CS
authors.lastname: Chow
authors.forename: Philip R
authors.initials: PR
authors.lastname: Cunningham
authors.forename: KangSeok
authors.initials: K
authors.lastname: Lee
authors.forename: May
authors.initials: M
authors.lastname: Meroueh
authors.forename: John
authors.initials: J
authors.lastname: SantaLucia
authors.forename: Shikha
authors.initials: S
authors.lastname: Varma
mesh_terms: D001482:Base Composition; D020137:Base Pair Mismatch; D001483:Base Sequence; D001665:Binding Sites; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006150:Guanine Nucleotides / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009942:Organometallic Compounds / Q000737:chemistry*; D010759:Phosphorus Isotopes; D010777:Photochemistry; D012313:RNA / Q000737:chemistry*; D012329:RNA, Bacterial / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D012343:RNA, Transfer / Q000737:chemistry; D012238:Rhodium / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013816:Thermodynamics; D014500:Uracil Nucleotides / Q000737:chemistry*
chemical_list: D006150:Guanine Nucleotides; D009942:Organometallic Compounds; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014500:Uracil Nucleotides; D012313:RNA; D012343:RNA, Transfer; D012238:Rhodium