title: Expression profiling with oligonucleotide arrays: technologies and applications for neurobiology.
abstract: DNA microarrays have been used in applications ranging from the assignment of gene function to analytical uses in prognostics. However, the detection sensitivity, cross hybridization, and reproducibility of these arrays can affect experimental design and data interpretation. Moreover, several technologies are available for fabrication of oligonucleotide microarrays. We review these technologies and performance attributes and, with data sets generated from human brain RNA, present statistical tools and methods to analyze data quality and to mine and visualize the data. Our data show high reproducibility and should allow an investigator to discern biological and regional variability from differential expression. Although we have used brain RNA as a model system to illustrate some of these points, the oligonucleotide arrays and methods employed in this study can be used with cell lines, tissue sections, blood, and other fluids. To further demonstrate this point, we provide data generated from total RNA sample sizes of 200 ng.
authors.affiliation: Motorola Life Sciences, Northbrook, IL, USA. ats015@email.mot.com
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Sendera
authors.forename: David
authors.initials: D
authors.lastname: Dorris
authors.forename: Ramesh
authors.initials: R
authors.lastname: Ramakrishnan
authors.forename: Allen
authors.initials: A
authors.lastname: Nguyen
authors.forename: Dionisios
authors.initials: D
authors.lastname: Trakas
authors.forename: Abhijit
authors.initials: A
authors.lastname: Mazumder
mesh_terms: D001921:Brain / Q000502:physiology; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D009446:Neurobiology / Q000379:methods*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA; D015203:Reproducibility of Results
chemical_list: D012313:RNA