title: Gene discovery in the freshwater fish parasite Trypanosoma carassii: identification of trans-sialidase-like and mucin-like genes.
abstract: A total of 1,921 expressed sequence tags (ESTs) were obtained from bloodstream trypomastigotes of Trypanosoma carassii, a parasite of economic importance due to its high prevalence in fish farms. Analysis of the data set allowed us to identify a trans-sialidase (TS)-like gene and three ESTs coding for putative mucin-like genes. TS activity was detected in cell extracts of bloodstream trypomastigotes. We have also used the sequence information obtained to identify genes that have not been previously described in trypanosomatids. (Additional information on these ESTs can be found at http://genoma.unsam.edu.ar/projects/tca.)
authors.affiliation: Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús, Universidad Nacional de General San Martín-CONICET, San Martín, 1650 Buenos Aires, Argentina.
authors.forename: Fernán
authors.initials: F
authors.lastname: Agüero
authors.forename: Vanina
authors.initials: V
authors.lastname: Campo
authors.forename: Laura
authors.initials: L
authors.lastname: Cremona
authors.forename: Adriana
authors.initials: A
authors.lastname: Jäger
authors.forename: Javier M
authors.initials: JM
authors.lastname: Di Noia
authors.forename: Peter
authors.initials: P
authors.lastname: Overath
authors.forename: Daniel O
authors.initials: DO
authors.lastname: Sánchez
authors.forename: Alberto Carlos
authors.initials: AC
authors.lastname: Frasch
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020224:Expressed Sequence Tags; D005393:Fish Diseases / Q000469:parasitology*; D005399:Fishes / Q000469:parasitology; D005618:Fresh Water; D006023:Glycoproteins; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics*; D009439:Neuraminidase / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D014345:Trypanosoma / Q000201:enzymology* / Q000235:genetics; D014352:Trypanosomiasis / Q000469:parasitology / Q000662:veterinary*
chemical_list: D006023:Glycoproteins; D009077:Mucins; D015800:Protozoan Proteins; C069715:trans-sialidase; D009439:Neuraminidase