title: Early impairment of insulin secretion in rats after surgical trauma.
abstract: OBJECTIVE
  Hyperglycaemia associated with insulin resistance is common after trauma and surgical procedures. Both reduced insulin sensitivity and altered insulin secretion may contribute to the impaired glucose homeostasis. We have demonstrated that skeletal muscle insulin resistance is present 2 h after small intestinal resection in rats. In this study, the aim was to investigate insulin secretion in the same experimental model.
  
  
  METHODS
  Small intestinal resection (5 cm) was performed in adult rats. The control animals underwent anaesthesia only.
  
  
  METHODS
  The intravenous glucose tolerance test (IVGTT), the hyperglycaemic clamp and in vitro studies in isolated pancreatic islets were performed after surgery. Concentrations of blood glucose, plasma insulin, corticosterone and interleukin-6 (IL-6) were determined 0-5 h postoperatively.
  
  
  RESULTS
  The insulin response in the IVGTT was attenuated 2 h (P<0.05) but not 4 h or during the hyperglycaemic clamp (3.5-4.5 h) postoperatively. Insulin secretion in response to glucose in vitro was decreased 2 h after the surgery (P<0.05), but no change was seen in arginine-stimulated secretion. Plasma levels of corticosterone were increased 3.5-5 h postoperatively (P<0.001-0.05). Increases in IL-6 were also seen postoperatively.
  
  
  CONCLUSIONS
  We demonstrate that glucose-induced, but not arginine-induced, insulin secretion is temporarily impaired after intestinal resection in rats. The later appearance of elevated corticosterone and IL-6 levels, as well as the preservation of the beta-cell inhibition in vitro, argues against the possibility that these two circulating factors are causally responsible for reduced insulin release seen after surgery in this model.
authors.affiliation: Arvid Wretlinds Laboratory for Metabolic and Nutritional Research, Department of Surgery, Karolinska Institutet at Huddinge University Hospital, 141 86 Stockholm, Sweden. Lisa.Strommer@cfss.ki.se
authors.forename: Lisa
authors.initials: L
authors.lastname: Strömmer
authors.forename: Malin
authors.initials: M
authors.lastname: Wickbom
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Margery K
authors.initials: MK
authors.lastname: Herrington
authors.forename: Claes-Göran
authors.initials: CG
authors.lastname: Ostenson
authors.forename: Urban
authors.initials: U
authors.lastname: Arnelo
authors.forename: Johan
authors.initials: J
authors.lastname: Permert
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology; D003345:Corticosterone / Q000097:blood; D013505:Digestive System Surgical Procedures / Q000009:adverse effects*; D005951:Glucose Tolerance Test; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007421:Intestine, Small / Q000601:surgery*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007328:Insulin; D001120:Arginine; D003345:Corticosterone