Label-free bioaffinity detection using terahertz technology.
We report the first use of differential terahertz time-domain spectroscopy for bioaffinity sensing. Binding is observed by measuring the transmission of a thin layer of biotin bound to the sensor protein avidin. We demonstrate the THz wave transmission of a sub-micron-thick film and sensitivity to 0.1 microg cm(-2) of biotin. These results point the way for a host of biosensor applications using T-rays, or pulsed far-infrared (FIR) radiation.
Mickan
Samuel P
SP
Center for Terahertz Research, Department of Physics, Applied Physics & Astronomy, Rensselaer Polytechnic Institute, NY 12180, USA.
Menikh
Abdellah
A
Liu
Haibo
H
Mannella
Carmen A
CA
MacColl
Robert
R
Abbott
Derek
D
Munch
Jesper
J
Zhang
X C
XC
D000345:Affinity Labels; D001360:Avidin / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001710:Biotin / Q000032:analysis / Q000737:chemistry*; D055590:Electromagnetic Phenomena / Q000295:instrumentation; D004867:Equipment Design; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D008872:Microwaves; D011485:Protein Binding; D012031:Refractometry / Q000295:instrumentation / Q000379:methods; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods; D013499:Surface Properties
D000345:Affinity Labels; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D001360:Avidin; D001710:Biotin