title: PCR detection of DNAs of animal origin in feed by primers based on sequences of short and long interspersed repetitive elements.
abstract: PCR primers for the detection of materials derived from ruminants, pigs, and chickens were newly designed on the basis of sequences of the Art2 short interspersed repetitive element (SINE), PRE-1 SINE, and CR1 long interspersed repetitive element (LINE), respectively. These primers amplified the SINE or LINE from total DNA extracted from the target animals and from test feed containing commercial meat and bone meal (MBM). With the primers, detection of Art2, PRE-1, or CR1 in test feed at concentrations of 0.01% MBM or less was possible. This method was suitable for the detection of microcontamination of feed by animal materials.
authors.affiliation: National Institute of Livestock and Grassland Science, P.O. Box 5, Tsukuba, Norindanchi, Ibaraki 305-0901, Japan. ktajima@affrc.go.jp
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Tajima
authors.forename: Osamu
authors.initials: O
authors.lastname: Enishi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Amari
authors.forename: Makoto
authors.initials: M
authors.lastname: Mitsumori
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kajikawa
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Kurihara
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yanai
authors.forename: Hiroki
authors.initials: H
authors.lastname: Matsui
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yasue
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Mitsuhashi
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kawashima
authors.forename: Mitsuto
authors.initials: M
authors.lastname: Matsumoto
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002645:Chickens; D004247:DNA / Q000032:analysis*; D017931:DNA Primers / Q000032:analysis; D005399:Fishes; D006041:Goats; D006736:Horses; D020071:Interspersed Repetitive Sequences; D008969:Molecular Sequence Data; D011784:Quail; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012756:Sheep; D013552:Swine
chemical_list: D017931:DNA Primers; D004247:DNA