Revealing of the spin-spin interaction due to incorporating a spin label in the carbohydrate parts of immuniglobulins M and G.
The EPR spectra of the preparations produced by spin labeling of the carbohydrate parts in monoclonal IgM and normal IgG with 2,2,6,6-tetramethyl-4-aminopiperidine-1-oxyl as the spin label indicate the existence of a rapid spin-spin exchange interaction between two spin labels. In the case of spin-labeled IgM, the carrier of such a spectrum is shown to be a glycopeptide noncovalently bound to IgM; it includes two spin labels and may be detached from the macromolecule by a combination of dialysis and gel filtration.
Timofeev
V P
VP
V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 117984, Russia. tim@genome.eimb.relarn.ru
Nikolsky
D O
DO
Lapuk
V A
VA
Alyoshkin
V A
VA
D002241:Carbohydrates / Q000737:chemistry*; D002850:Chromatography, Gel; D003956:Dialysis; D004578:Electron Spin Resonance Spectroscopy; D006020:Glycopeptides / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000032:analysis / Q000737:chemistry*; D007075:Immunoglobulin M / Q000032:analysis / Q000737:chemistry*; D009589:Nitrogen Oxides / Q000737:chemistry; D011487:Protein Conformation; D013113:Spin Labels; D013696:Temperature
D002241:Carbohydrates; D006020:Glycopeptides; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009589:Nitrogen Oxides; D013113:Spin Labels