Short-term modulation of GABAA receptor function in the adult female rat.
Oxytocin neurons in the supraoptic nucleus (SON) exhibit marked neuronal plasticity during each reproductive cycle. We have previously shown that this neuronal plasticity includes GABAA receptor subunit switching around the time of parturition. Here we focus on addition plasticity in short-term regulatory mechanisms of postsynaptic receptor function before and after parturition, i.e. alterations in metabotropic and allosteric modulation of GABAA receptor activity. Both short- and long-term regulation of the GABAA receptor function affects the electrical behaviour of the oxytocin neurons (Brussaard and Herbison, 2000); however, their causal linkage until recently remained unclear. Non-genomic gonadal steroid feedback to oxytocin neurons is mediated via the neurosteroid allopregnanolone (3 alpha-OH-DHP) that is an allosteric modulator of postsynaptic GABAA receptors. We recently found evidence to support the idea that (1) neurosteroids not only potentiate GABAA receptor function but also prevent its suppression by PKC (Brussaard et al., 2000), and (2) that neurosteroid sensitivity of GABAA receptor is not regulated by subunit switching, but instead, is dependent on the balance between endogenous phosphatase and PKC activity (Koksma et al., 2002). Thus, before pregnancy, the GABAA receptors are sensitive to 3 alpha-OH-DHP, due to a constitutively high level of phosphatase activity. At parturition, endogenous release of oxytocin within the SON shifts the intracellular balance towards a higher level of phosphorylation, leading to 3 alpha-OH-DHP insensitivity of the GABAA receptors. Here we discuss the putative mechanisms underlying these changes in receptor physiology, their causal relations and the functional significance for the hormonal output.
Brussaard
Arjen B
AB
Department of Experimental Neurophysiology, Vrije Universiteit Amsterdam, Research Institute Neurosciences, Centre for Neurogenomics and Cognitive Research, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands. brssrd@bio.vu.nl
Koksma
Jan-Jurjen
JJ
D000818:Animals; D005260:Female; D009474:Neurons / Q000502:physiology*; D011247:Pregnancy; D051381:Rats; D011963:Receptors, GABA-A / Q000502:physiology*; D013495:Supraoptic Nucleus / Q000502:physiology
D011963:Receptors, GABA-A