title: Topology of the 10 subunits within the decamer of KLH, the hemocyanin of the marine gastropod Megathura crenulata.
abstract: Immunoelectron microscopy has been performed using negatively stained immune complexes of keyhole limpet hemocyanin isoform 1 (KLH1) decamers and a functional unit-specific monoclonal antibody anti-KLH1-c1. The antibody links hemocyanin molecules at both the collar and the collarless edge of the decamer, indicating a peripheral localization of functional units c. In isoform 2 (KLH2) the positions of functional units c have been identified with the peanut agglutinin (PNA), which has previously been shown to exclusively bind to KLH2-c. Ferritin linked to PNA was used to visualize labeled molecules electron microscopically. The pattern of labeling also indicates a peripheral localization of the c functional units. The data presented in this paper support only one of two possible models for the subunit orientation within the hemocyanin decamer.
authors.affiliation: Institute of Zoology, University of Mainz, Mainz, Germany. gebauer@mail.uni-mainz.de
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Gebauer
authors.forename: J
authors.initials: J
authors.lastname: Robin Harris
authors.forename: Jürgen
authors.initials: J
authors.lastname: Markl
mesh_terms: D000818:Animals; D018360:Crystallography, X-Ray; D006433:Hemocyanins / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure; D008854:Microscopy, Electron; D008958:Models, Molecular; D008970:Molecular Weight; D008974:Mollusca / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D021122:Protein Subunits / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D021122:Protein Subunits; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin