[Expression of NF-kappa B in human bladder cancer and its clinical significance].
OBJECTIVE
Studies on solid cancer(such as breast cancer, hepatocellular cancer, pancreatic cancer) indicated that the abnormal expression of nuclear transcription factor Kappa B (NF-kappa B) regulates angiogenesis and cyclin-related genes. This study was designed to investigate the expression differences of NF-kappa B and its regulated genes between human primary transitional cell carcinoma(TCCs) of bladder and non-cancer bladder mucosa and its clinical significance.


METHODS
Forty-three frozen sections including 30 bladder cancer and 13 non-cancer bladder mucosa were subjected to immunohistochemistry and nucleus staining for determining levels of NF-kappa B family and I kappa B alpha; Five paired cancer and non-cancer specimens were subjected to Western blot for analysis p65, an important subtype of NF-kappa B; Thirteen paired specimens were subjected to RT-PCR for determination mRNA levels of p50, p52, p65, c-Rel, RelB, I kappa B alpha, CyclinD1, IL-8.


RESULTS
Expressions of p50, p52, p65, c-Rel, RelB, I kappa B alpha, CyclinD1, IL-8 mRNAs in bladder cancer were higher than that in non-cancer bladder mucosa (P < 0.01, P < 0.05, P < 0.01, P < 0.01, P < 0.05, P < 0.05, P < 0.05 and P < 0.05, respectively). Nucelus stainings of p50, p52, p65, c-Rel, RelB were also stronger in bladder cancer(P < 0.01, P < 0.01, P < 0.01, P < 0.01 and P < 0.01, respectively). Moreover, p65 was expressed more in cancer tissue than that in non-cancer mucosa evidenced by Western blot. In bladder cancer, the ranking score differences of p52, p65, c-Rel protein between lymphatic metastasis group and non-lymphatic metastasis group were statistically significant (P < 0.01, P < 0.01, P < 0.05, respectively).


CONCLUSIONS
Compared to noncancer bladder mucosa, expressions of NF-kappa B family and its regulated genes in bladder cancer are markedly higher. NF-kappa B may be related to lymphatic metastasis.
Xie
Dong-hua
DH
Department of Urology, First People's Hospital of Shanghai, Shanghai 200080, P. R. China. xiedh@online.sh.cn
Tang
Xiao-da
XD
Xia
Shu-jie
SJ
Tan
Jian-ming
JM
Wang
Xiang-hui
XH
Cai
Yong
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D019938:Cyclin D1 / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D006801:Humans; D020672:I-kappa B Proteins / Q000096:biosynthesis / Q000235:genetics; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000096:biosynthesis* / Q000235:genetics; D052002:NF-kappa B p50 Subunit; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics; D020569:Proto-Oncogene Proteins c-rel / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051996:Transcription Factor RelA; D051841:Transcription Factor RelB; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology
D020672:I-kappa B Proteins; D016209:Interleukin-8; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; C000606204:NFKBIA protein, human; D011518:Proto-Oncogene Proteins; D020569:Proto-Oncogene Proteins c-rel; C497593:RELB protein, human; D051996:Transcription Factor RelA; D014157:Transcription Factors; D019938:Cyclin D1; D000072000:NF-KappaB Inhibitor alpha; D051841:Transcription Factor RelB