High incidence of Hox11L2 expression in children with T-ALL.
The orphan homeobox gene HOX11L2 was previously found to be transcriptionally activated as a result of the t(5;14)(q35;q32) translocation in three T-ALL cases. We now tested by RT-PCR Hox11L2 expression in 23 consecutive cases of T-ALL (15 children aged 0.8-14 years, eight adults aged 17-55 years) and as control 13 B-ALL patients from a single institution. Hox11L2 expression was undetectable in all patients with B-ALL, nor in adults with T-ALL. Nine children (60% of the cases), all boys, expressed Hox11L2. Blast cells from most of the latter patients carried surface CD1a, CD10 and not CD34 antigens, in contrast to the other children. FISH, M-FISH and IPM-FISH analysis failed to detect a t(5;14)(q35;q32) in one of them, which suggests a possible distinct genetic mechanism in Hox11L2 expression induction. Hence, Hox11L2 expression seems to be the most frequent abnormality in childhood T-ALL to date, comparable to the t(12;21) in child B-ALL.
Mauvieux
L
L
Laboratoire d'Hématologie, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.
Leymarie
V
V
Helias
C
C
Perrusson
N
N
Falkenrodt
A
A
Lioure
B
B
Lutz
P
P
Lessard
M
M
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002883:Chromosomes, Human, Pair 14; D002895:Chromosomes, Human, Pair 5; D005260:Female; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D016130:Immunophenotyping; D015994:Incidence; D007223:Infant; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics* / Q000401:mortality; D008297:Male; D015513:Oncogene Proteins / Q000235:genetics*; D011518:Proto-Oncogene Proteins; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis; D015996:Survival Rate; D014178:Translocation, Genetic
D015703:Antigens, CD; D018398:Homeodomain Proteins; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; C489494:TLX3 protein, human; C071250:TLX1 protein, human