Down-regulation of heart HFABP and UCP2 gene expression in diet-induced (cafeteria) obese rats.
Long-term exposure to hypercaloric high fat diet induced marked tissue fatty acid accumulation and may influence cell function. Previous results in our laboratory showed that uncoupling proteins (UCPs) and fatty acid-binding protein (FABP) gene expression are changed in adipose tissue and skeletal muscle tissue in diet-induced (cafeteria) obese animals. The aim of this study was to examine heart FABP (HFABP) and UCP2 gene expressions in dietary obese rats. Rats fed on a high-fat diet for 65 days had significantly higher fat stores and body weight than control rats. Interestingly, we found that both HFABP and UCP2 mRNA levels were significantly reduced in cafeteria-obese rats when compared to control animals. Moreover, a statistically significant correlation was observed between the two gene expression levels. The down-regulation of heart HFABP and UCP2 parallels the lower lipid utilization which may account for an enhanced fat deposition. It is plausible that these two genes are regulated by the same family of transcription factors.
Marti
A
A
Dpto de Fisiología y Nutrición, Universidad de Navarra, Pamplona, Spain. amarti@unav.es
Vaquerizo
J
J
Zulet
M A
MA
Moreno-Aliaga
M J
MJ
Martínez
J A
JA
D000818:Animals; D015415:Biomarkers / Q000097:blood; D001835:Body Weight; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics*; D017931:DNA Primers; D004032:Diet; D004041:Dietary Fats; D015536:Down-Regulation; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D007473:Ion Channels; D008297:Male; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D009206:Myocardium / Q000378:metabolism*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D009765:Obesity / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D009929:Organ Size; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013223:Statistics as Topic; D000071246:Uncoupling Protein 2
D015415:Biomarkers; D002352:Carrier Proteins; D017931:DNA Primers; D004041:Dietary Fats; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D007473:Ion Channels; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D012333:RNA, Messenger; C000604391:Ucp2 protein, rat; D000071246:Uncoupling Protein 2