Enantiomeric separation of methamphetamine and related analogs by capillary zone electrophoresis: intelligence study in routine methamphetamine seizures.
A method for simultaneous enantiomeric separation of ephedrine, pseudoephedrine, and methamphetamine (MA) in a single run by simple capillary zone electrophoresis (CZE) with beta-cyclodextrin as a chiral selector is described. The effects of the buffer pH, phosphate concentration, beta-cyclodextrin concentration, voltage and temperature on the peak resolution were examined. Good enantiomeric resolution was attained for each analyte under our optimized conditions: 15 mM beta-cyclodextrin, 300 mM NaH2PO4 at pH 2.5 with an uncoated capillary (64.5 cm x 50 microm), applied potential at 20 kV and temperature at 30 degrees C. Ultraviolet (UV) detection at a fixed wavelength (200 nm) was employed using a diode array detector. Using phentermine as an internal standard, migration times for all analytes are reproducible within 0.16% for intra-day and 0.6% for inter-day runs. Application of this method to the analysis of confiscated drugs is discussed.
Cheng
Wing-Chi
WC
Forensic Science Division, Government Laboratory, Homantin Government Offices, Homantin, Hong Kong Special Administrative Region, People's Republic of China.
Lee
Wing-Man
WM
Chan
Man-Fai
MF
Tsui
Pui
P
Dao
Kwok-leung
KL
D003505:Cyclodextrins / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D013287:Illicit Drugs / Q000302:isolation & purification*; D007202:Indicators and Reagents; D008694:Methamphetamine / Q000031:analogs & derivatives / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D003505:Cyclodextrins; D013287:Illicit Drugs; D007202:Indicators and Reagents; D008694:Methamphetamine