Serum deprivation increases the expression of low density lipoprotein receptor-related protein in primary cultured rat astrocytes.
The low density lipoprotein receptor (LDLR)-related protein (LRP) is a multifunctional receptor which mediates the endocytic uptake of several ligands implicated in Alzheimer's disease pathophysiology. Although LRP, as a member of the LDLR family, is likely to be regulated in response to various cellular stresses, this regulation has not been fully understood yet. In the present study we studied the regulation of LRP expression in primary cultured rat astrocytes in response to serum deprivation as a general cellular stress. A significant increase in LRP expression was detected after serum deprivation and this increase was blocked by treatment of U0126, an inhibitor of MAP kinase. This serum deprivation action was partially reversed by either serum or D-glucose supplementation, but further augmented by glutamine. This result contrasted with a finding that glutamine suppressed gadd153 protein induced by serum deprivation. Taken together, the present data suggest that serum deprivation induces dramatically LRP expression in astrocytes partly by MAPK signaling pathways and by signaling pathways apparently distinct from gadd153 induction.
Jo
Inho
I
Department of Biomedical Sciences, National Institute of Health, 5 Nokbun-dong, Eunpyung-gu, 122-701, Seoul, Republic of Korea.
Im
Hyung Min
HM
Shin
Hyun Ju
HJ
Won Cho
Kae
K
Jung
Miyoung
M
Kim
Sun Don
SD
Kim Jeong
Jeongmi
J
Ahn Jo
Sangmee
S
D036002:ADP Ribose Transferases / Q000494:pharmacology; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001427:Bacterial Toxins / Q000494:pharmacology; D015152:Blotting, Northern; D015153:Blotting, Western; D002070:Butadienes / Q000494:pharmacology; D002470:Cell Survival; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005098:Exotoxins / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D005973:Glutamine / Q000494:pharmacology; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000096:biosynthesis*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013997:Time Factors; D015854:Up-Regulation; D037521:Virulence Factors / Q000494:pharmacology; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D002070:Butadienes; D016895:Culture Media, Serum-Free; D004791:Enzyme Inhibitors; D005098:Exotoxins; D005947:Glucose; D005973:Glutamine; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D020928:Mitogen-Activated Protein Kinases; D009570:Nitriles; D012333:RNA, Messenger; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A; C113580:U 0126