Cloning, expression and characterization of a novel human VMP gene.
We report here cloning and characterization of a novel human gene, termed VMP, which is a vesicular membrane protein. RT-PCR analysis shows that VMP is expressed exclusively in brain of the 16 tissues examined, suggesting that it is a neuron-specific membrane protein. The cDNA encodes 195 amino acid with a putative molecular weight of about 24 KDa. VMP contains two putative membrane spanning domains and a hydrophilic tail homologous to the microtubule-binding domain of MAPs. So it is speculated that VMP may associated with microtubules through its C-terminal and plays an important role in vesicular organelles transport and nerve signals.
Cheng
Chao
C
Xu
Jian
J
Ye
Xin
X
Dai
Jianfeng
J
Wu
Qihan
Q
Zeng
Li
L
Wang
Liu
L
Zhao
Wei
W
Ji
Chaoneng
C
Gu
Shaohua
S
Xie
Yi
Y
Mao
Yumin
Y
D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009474:Neurons / Q000737:chemistry; D017434:Protein Structure, Tertiary; D014018:Tissue Distribution
D018076:DNA, Complementary; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; C046375:NRSN1 protein, human