[Influence of selective cyclooxygenase-2 inhibitor on proliferation of human gastric cancer cells].
OBJECTIVE
Accumulating evidence indicates that nonsteroidal antiinflammatory drugs (NSAIDs) may reduce the risk of digestive system tumors, their chemopreventive effects appear to be due to inhibition of cyclooxygenase-2 (COX-2). At present, influence of selective COX-2 inhibitor on proliferation of human gastric cancer cells and mechanism were not very clear. The current study was designed to evaluate the potential role of Nimesulide, a selective COX-2 inhibitor, on the PGE2 level, proliferation and apoptosis of gastric adenocarcinoma cell line SGC7901.


METHODS
MTT assay and radioimmunoassay were used to determine the influence of Nimesulide on the proliferation of SGC7901 cells and PGE2 release in the culture supernatant; Transmission electron microscopy and flow cytometry were used to observe the induction of Nimesulide on the apoptosis of SGC7901 cells and influence on the distribution of cell cycle.


RESULTS
Nimesulide inhibited the cells proliferation in a time- and dose-dependent fashion and reduced the release of PGE2, induced apoptosis of the cells in a dose-dependent and non-linear manner and increased the proportion of cells in the G0/G1 phase.


CONCLUSIONS
Nimesulide may inhibit the proliferation of gastric adenocarcinoma cells SGC7901 through decreasing PGE2 release, and affecting the distribution of cell cycle and inducing apoptosis. Selective COX-2 inhibitor may be a new way of the chemoprevention and chemotherapy for gastric carcinoma.
Li
Jian-ying
JY
Department of Gastroenterology, People's Hospital, Wuhan University, Wuhan 430060, P. R. China.
Yu
Jie-ping
JP
Luo
He-sheng
HS
Yu
Bao-ping
BP
Tian
Geng
G
Mei
Qiao
Q
Huang
Jie-an
JA
D000230:Adenocarcinoma; D017209:Apoptosis; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D051546:Cyclooxygenase 2; D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D015232:Dinoprostone / Q000378:metabolism; D007527:Isoenzymes / Q000037:antagonists & inhibitors*; D011451:Prostaglandin-Endoperoxide Synthases; D013274:Stomach Neoplasms; D013449:Sulfonamides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors; D007527:Isoenzymes; D013449:Sulfonamides; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone; C012655:nimesulide