title: The non-ionic detergent Brij 58P mimics chaperone effects.
abstract: The non-ionic detergent Brij 58P is recommended as a stabilizing agent for protein storage; for example, the aggregation-prone chaperone DnaJ can be maintained in solution by low concentrations of Brij 58P. During protein folding studies with alpha-glucosidase, rhodanese and citrate synthase as model proteins, we discovered that the low concentrations of Brij 58P usually added with purified DnaJ to renaturation samples are sufficient to mimic chaperone effects with respect to prevention of protein aggregation. Furthermore, addition of Brij 58P to refolding alpha-glucosidase and citrate synthase enhanced the yield of refolded protein by a factor of two. Thus, Brij 58P can mimic chaperone effects and care should be taken when the substance is used to stabilize chaperone preparations.
authors.affiliation: Institute for Biotechnology, Martin-Luther-Universität Halle-Wittenberg, Kurt-Mothes-Strasse 3, 06120 Halle, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Krause
authors.forename: Rainer
authors.initials: R
authors.lastname: Rudolph
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Schwarz
mesh_terms: D002950:Citrate (si)-Synthase / Q000737:chemistry / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004795:Enzyme Stability / Q000187:drug effects; D005079:Excipients / Q000494:pharmacology*; D007700:Kinetics; D018832:Molecular Chaperones / Q000494:pharmacology; D011489:Protein Denaturation; D017510:Protein Folding; D000520:alpha-Glucosidases / Q000737:chemistry / Q000378:metabolism
chemical_list: D003902:Detergents; D005079:Excipients; D018832:Molecular Chaperones; D002950:Citrate (si)-Synthase; D000520:alpha-Glucosidases