Microarray analysis of shear stressed endothelial cells.
The cDNA microarray is an extremely beneficial tool for study of differential gene expression in the cardiovascular system. This technique is used in many different applications including drug discovery, environmental science, and the effects of mechanical forces on vascular cell phenotype. The paper reviews work by others, and describes our study on effects of shear stress on vascular endothelial cells. These microarray studies verified earlier findings using Northern and polymerase chain reaction (PCR) analyses in this area; and also found previously unidentified differentially expressed genes, leading to new hypotheses regarding how cells and tissues respond to biochemical and mechanical stimuli.
McCormick
Susan M
SM
Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, USA.
Frye
Stacie R
SR
Eskin
Suzanne G
SG
Teng
Christina L
CL
Lu
Chiung-Mei
CM
Russell
Christopher G
CG
Chittur
Krishnan K
KK
McIntire
Larry V
LV
D000818:Animals; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D013314:Stress, Mechanical