Enhancement of nestin protein-immunoreactivity induced by ionizing radiation in the forebrain ependymal regions of rats.
Expression of nestin was immunohistochemically examined in the forebrains of rats receiving ionizing radiation. Nestin-immunoreactive cells were predominately distributed in ependymal regions. Nestin-immunoreactivity in ependymal regions of irradiated rats increased significantly from 1 to 4 weeks after ionizing radiation compared with that of controls. Double immunofluorescence confirmed that about 94% of nestin-positive cells exhibited glial fibrillary acidic protein-immunoreactivity and a minor population of them showed Ki-67-immunoreactivity in these regions. The results have provided evidence for up-regulation of nestin expression induced by ionizing radiation in ependymal cells, suggesting that these reactive ependymal cells may be involved in remodeling and repairing processes of brain irradiation injury.
Shi
Mei
M
Department of Radiotherapy, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, PR China.
Wei
Li-Chun
LC
Cao
Rong
R
Chen
Liang-Wei
LW
D000818:Animals; D001930:Brain Injuries / Q000378:metabolism / Q000503:physiopathology; D002552:Cerebral Ventricles / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D004805:Ependyma / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism / Q000528:radiation effects; D007381:Intermediate Filament Proteins / Q000378:metabolism* / Q000528:radiation effects; D019394:Ki-67 Antigen / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology* / Q000528:radiation effects; D009419:Nerve Tissue Proteins; D064231:Nestin; D016548:Prosencephalon / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D011839:Radiation, Ionizing; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D015854:Up-Regulation / Q000502:physiology* / Q000528:radiation effects
D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D019394:Ki-67 Antigen; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin