[Reaction kinetics of hydrolysis of animal fat by pancreatic lipase].
Hydrolysis of lipids from beef fat by pancreatic lipase was studied. Maximal release of free fatty acids was shown to take place for the first 3 h of experiment. After this, transetherification developed. Following most important time course parameters were determined: maximal hydrolysis rate V = 1.25 +/- 0.1 mg fat/(ml min), Michaelis constant KMH = 100 +/- 12 mg fat/ml, constant of substrate inhibition KS = 10.0 +/- 0.8 mg fat/ml, equilibrium constant KP = 277 +/- 170 mg fat/ml, and activation energy of beef fat hydrolysis by pancreatic lipase Ea = 19.1 +/- 1.1 kJ/mole. The time course method used could be applied to develop biotransformation of poorly assimilated fats to more valuable products.
German
A B
AB
Moscow State University of Forestry, 141001, Mytishchi, Moscow Oblast. aivankin@mgul.ac.ru
Nekliudov
A D
AD
Ivankin
A N
AN
Berdutina
A V
AV
D000818:Animals; D002417:Cattle; D005230:Fatty Acids, Nonesterified / Q000032:analysis / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D050356:Lipid Metabolism; D010179:Pancreas / Q000201:enzymology*
D005230:Fatty Acids, Nonesterified; D008049:Lipase