Tracheospasmolytic activity of viteosin-A and vitexicarpin isolated from vitex trifolia.
The n-hexane extract that has shown activity in the tracheospasmolytic bioassay was fractionated by solvent extraction and from the major active fraction two compounds were isolated and identified as viteosin-A and vitexicarpin. These compounds blocked spontaneous contraction of isolated male guinea pig trachea induced by histamine; however only vitexicarpin was active in a model using sensitized guinea pig trachea stimulated by ovalbumin up to minimum dose of 1.3 x 10(-5) M. The result suggests that vitexicarpin is able to block effects of histamine released from sensitized mast cells possibly by stabilizing the mast cells membrane function.
Alam
Gemini
G
Wahyuono
Subagus
S
Ganjar
Ibnu G
IG
Hakim
Lukman
L
Timmerman
H
H
Verpoorte
Rob
R
D000818:Animals; D001986:Bronchial Spasm / Q000139:chemically induced / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology*; D006168:Guinea Pigs; D006632:Histamine; D007546:Isoquinolines / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D010047:Ovalbumin; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D018515:Plant Leaves; D014132:Trachea / Q000187:drug effects*; D020950:Vitex
D005419:Flavonoids; D007546:Isoquinolines; D010936:Plant Extracts; C470478:curine; C054133:casticin; D006632:Histamine; D010047:Ovalbumin