Chronic nicotine and smoking exposure decreases GABA(B1) receptor expression in the rat hippocampus.
Nicotine and smoking have long been proved to play an important role in cognition and memory in the hippocampus. This effect is closely related to the gamma-aminobutyric acid (GABA)ergic system. Previous research has focused on functional and pharmacological aspects of nicotine's modulation activity. In this study, the effects of nicotine and different doses of smoking on GABA(B1) expression in the rat hippocampus have been examined using in situ hybridization and RNase protection assay. GABA(B1) receptor mRNAs were intensely expressed in the CA1, CA2, CA3, and dentate gyrus areas of the hippocampus. Nicotine and smoking doses dependently decreased GABA(B1) receptor expression in the hippocampus. These results revealed new aspects of nicotine's modulation on GABA(B) receptor, and on learning and memory.
Li
Shu P
SP
Division of Life Science and Applied Life Science, College of Natural Sciences, Gyeongsang National University, Gazwa-dong 900, Chinju, Gyeongnam, South Korea.
Park
Moon S
MS
Bahk
Jong Y
JY
Kim
Myeong O
MO
D000818:Animals; D015870:Gene Expression / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D017403:In Situ Hybridization; D008297:Male; D009538:Nicotine / Q000633:toxicity*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D018080:Receptors, GABA-B / Q000096:biosynthesis / Q000187:drug effects* / Q000235:genetics; D012907:Smoking / Q000378:metabolism*; D014028:Tobacco Smoke Pollution
D012333:RNA, Messenger; D018080:Receptors, GABA-B; D014028:Tobacco Smoke Pollution; D009538:Nicotine