Analysis of gene polymorphisms in the regulatory region of MCP-1, RANTES, and CCR5 in liver transplant recipients.
Chemokines and their receptors play a major role in the inflammatory and immune responses that mediate allograft outcome. The production of some chemokines varies among individuals and these variations may be determined by genetic polymorphisms, most commonly within the regulatory region of the gene. We investigated whether the functional polymorphisms of the chemokines RANTES, MCP-1 and chemokine receptor CCR5 are associated with the incidence of acute rejection and long-term liver graft survival. Two hundred nine liver transplant recipients were genotyped using polymerase chain reaction sequence-specific primers for the following polymorphisms: RANTES-28, MCP-1 -2518, and CCR5-59029. There was no association with any of the three genotypes and the incidence of acute rejection episodes. In addition, no association of RANTES-28, MCP-1 -2518, or CCR5 -59029 variants with long-term liver graft survival was found. In conclusion, variants of RANTES-28, MCP-1 -2518, and CCR5-59029 neither influenced the incidence of acute rejection nor affected long-term allograft survival upon liver transplantation in the context of this analysis.
Schröppel
Bernd
B
Division of Nephrology, Mount Sinai School of Medicine, New York, New York 10029, USA. Bernd.Schroppel@mssm.edu
Fischereder
Michael
M
Lin
Marvin
M
Marder
Brad
B
Schiano
Tom
T
Krämer
Bernhard K
BK
Murphy
Barbara
B
D000328:Adult; D018932:Chemokine CCL2 / Q000235:genetics*; D018946:Chemokine CCL5 / Q000235:genetics*; D005260:Female; D006084:Graft Rejection / Q000235:genetics; D006085:Graft Survival / Q000235:genetics; D006801:Humans; D016031:Liver Transplantation / Q000401:mortality / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D019713:Receptors, CCR5 / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D012307:Risk Factors; D016896:Treatment Outcome
D018932:Chemokine CCL2; D018946:Chemokine CCL5; D019713:Receptors, CCR5