Molecular typing of Plasmodium falciparum from Giemsa-stained blood smears confirms nosocomial malaria transmission.
In this report, we describe the partial molecular characterisation of Plasmodium falciparum isolates obtained from two individuals who were involved in a probable case of accidental malaria transmission after admission to a hospital in the metropolitan area of São Paulo, Brazil. Molecular analysis of polymorphic stretches of the merozoite surface protein 1 and 2 genes using PCR-typing and nucleotide sequencing revealed that the two isolates were identical and that the identified msp-1 gene was different from all others published to date. Additional anamnestic data supported our findings and made all other possible routes of infection unlikely. The methodology used here is simple to perform and needs as little as one Giemsa-stained blood smear as starting material.
Kirchgatter
K
K
Núcleo de Estudos em Malária, Superintendência de Controle de Endemias (SUCEN), Av Dr Eneas de Carvalho Aguiar 470, Cerqueira César, SP São Paulo 05403-000, Brazil.
Wunderlich
G
G
Branquinho
M S
MS
Salles
T M
TM
Lian
Y C
YC
Carneiro-Junior
R A
RA
Di Santi
S M
SM
D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000302:isolation & purification; D001399:Azure Stains; D001483:Base Sequence; D002675:Child, Preschool; D003428:Cross Infection / Q000175:diagnosis / Q000635:transmission*; D005260:Female; D006403:Hematologic Tests; D006801:Humans; D016778:Malaria, Falciparum / Q000175:diagnosis / Q000635:transmission*; D008297:Male; D020066:Merozoite Surface Protein 1 / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D015800:Protozoan Proteins / Q000235:genetics / Q000302:isolation & purification
D000953:Antigens, Protozoan; D001399:Azure Stains; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium