title: Enzyme histochemistry is useful to assess viability of tumor tissue after microwave coagulation therapy (MCT): metastatic adenocarcinoma treated by lateral segmentectomy after MCT.
abstract: We report on a case of metastatic adenocarcinoma of liver that was removed and examined histochemically after microwave coagulation therapy (MCT). The patient was a 65-year-old woman who had a metastatic tumor in the liver (S3) after high anterior resection due to a rectal adenocarcinoma and received MCT against the tumor. One month after MCT, multiple metastatic tumors were detected by abdominal computed tomography (CT) scan. As it was difficult to control them by MCT alone, we performed lateral segmentectomy. To assess the effects of microwave ablation on cellular viability of metastatic tumor, we used enzyme histochemistry for acid phosphatase (AcP), which is positive in macrophages infiltrating in the tumor. In a part of the ablated area of resected liver, there was remaining neoplastic tissue of which the morphology was maintained in H&E staining. This was found to be microwave-fixed non-viable tissue because no enzyme activity of AcP was detected in the infiltrating macrophages. This case report suggests that enzyme histochemistry was useful to assess the effect of MCT, enabling us to distinguish fixed cells from viable cells.
authors.affiliation: First department of Surgery, Shiga University of Medical Science, Shiga, Ohtsu, Japan.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Mukaisho
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Kurumi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sugihara
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Naka
authors.forename: Sumihiro
authors.initials: S
authors.lastname: Kamitani
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tsubosa
authors.forename: Suzuko
authors.initials: S
authors.lastname: Moritani
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Endo
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Hanasawa
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Morikawa
authors.forename: Toshiro
authors.initials: T
authors.lastname: Inubushi
authors.forename: Takanori
authors.initials: T
authors.lastname: Hattori
authors.forename: Tohru
authors.initials: T
authors.lastname: Tani
mesh_terms: D000135:Acid Phosphatase; D000230:Adenocarcinoma / Q000473:pathology / Q000556:secondary* / Q000601:surgery / Q000628:therapy*; D000368:Aged; D004564:Electrocoagulation; D005260:Female; D005629:Frozen Sections; D006498:Hepatectomy; D006651:Histocytochemistry; D006801:Humans; D008099:Liver / Q000201:enzymology / Q000528:radiation effects*; D008113:Liver Neoplasms / Q000473:pathology / Q000556:secondary* / Q000601:surgery / Q000628:therapy*; D008872:Microwaves / Q000627:therapeutic use*; D012004:Rectal Neoplasms / Q000473:pathology*
chemical_list: D000135:Acid Phosphatase