Flare-up reaction on murine contact hypersensitivity. III. Effect of staphylococcal enterotoxin B.
Staphylococcal enterotoxin B (SEB), a bacterial superantigen, is known as an immunomodulator because it activates an extremely large number of T-cells, and induces the production of large amounts of cytokines. In this study, we examined the effects of SEB on the contact hypersensitivity reaction (CHR). BALB/c mice were first sensitized through haptens applied to the back, and CHR was then induced through challenge to the left ear using the same haptens. SEB was administered intravenously 4 weeks later, causing a flare-up, peaking at 24 h post-administration, in the left ear that had previously exhibited CHR. This flare-up reaction was hapten non-specific, and was inhibited by anti-mouse tumor necrosis factor (TNF)-alpha antibodies. The flare-up was also suppressed by the oral administration of cyclosporin A prior to the administration of SEB. These results suggest that SEB induces a flare-up of CHR via the production of TNF-alpha.
Natsuaki
Masaru
M
Department of Dermatology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan.
Abe
Kayoko
K
Kitano
Yukio
Y
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D016572:Cyclosporine / Q000494:pharmacology; D003877:Dermatitis, Contact / Q000276:immunology / Q000503:physiopathology*; D004139:Dinitrofluorobenzene / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004423:Ear / Q000503:physiopathology; D004768:Enterotoxins / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006241:Haptens / Q000276:immunology; D007275:Injections, Intravenous; D051379:Mice; D008807:Mice, Inbred BALB C; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000276:immunology
D000906:Antibodies; D004768:Enterotoxins; D006241:Haptens; D014409:Tumor Necrosis Factor-alpha; C031818:enterotoxin B, staphylococcal; D016572:Cyclosporine; D004139:Dinitrofluorobenzene