Replication and transcription of viral RNAs by recombinant L proteins of New Jersey serotype of vesicular stomatitis virus.
The large (L) protein of vesicular stomatitis virus (VSV), catalytic subunit of RNA-dependent RNA polymerase is responsible for the transcription and replication of VSV. The L protein of the Indiana serotype of VSV (VSV(Ind)) has previously been cloned and expressed, and used in the reverse genetics of VSV(Ind). However, the cDNA clones expressing functional L proteins of the VSV(NJ) serotype were not available. It was necessary to obtain functional clones of the New Jersey serotype of VSV (VSV(NJ)) in order to study homologous viral interference. Here we report the cDNA cloning, expression, and functional analyses of L proteins from both the Hazelhurst subtype and Concan subtype of VSV(NJ). The analysis of the expressed L proteins for the transcription and replication of VSV demonstrate that both VSV(NJ) L clones express functional RNA-dependent RNA polymerase.
Kim
Gyoung Nyoun
GN
Department of Microbiology and Immunology, Faculty of Medicine and Dentistry, Siebens-Drake Research Institute, University of Western Ontario, London Ont, Canada N6G 2V4.
Choi
Woo-young
WY
Park
Manhoon
M
Kang
C Yong
CY
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012703:Serotyping; D014158:Transcription, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018116:Vesiculovirus; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
D018076:DNA, Complementary; D012367:RNA, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins; C040530:L protein, vesicular stomatitis virus; D012324:RNA-Dependent RNA Polymerase