B chromosomes of Korean field mouse Apodemus peninsulae (Rodentia, Murinae) analysed by microdissection and FISH.
Organization of B chromosomes in the Korean field mouse Apodemus peninsulae was analyzed. We painted its metaphase chromosomes with whole and partial chromosome paints generated by microdissection and DOP-PCR. The results of the painting indicated that all B chromosomes contained a large amount of repeated DNA sequences. The repeats could be classified in terms of their homology and predominant location. Pericentromeric repeats of B chromosomes were present in many copies in pericentromeric C-blocks of all autosomes and in non-centromeric C-blocks of the sex chromosomes. B arm specific type 1 repeats comprised the main body of the arms of almost all B chromosomes and were present in the arms of A chromosomes as interspersed sequences. B arm-specific type 2 repeats were found at the ends of some B chromosomes that did not undergo compaction at the interphase- metaphase transition and remained uncondensed. On the basis of comparative analysis of localization of B chromosome repeats in the chromosomes of two related species, A. peninsulae and A. agrarius, we suggest a hypothesis of B chromosome origin and evolution in the genus Apodemus.
Karamysheva
T V
TV
Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia.
Andreenkova
O V
OV
Bochkaerev
M N
MN
Borissov
Y M
YM
Bogdanchikova
N
N
Borodin
P M
PM
Rubtsov
N B
NB
D000818:Animals; D002874:Chromosome Mapping; D020223:Chromosome Painting / Q000379:methods; D002875:Chromosomes / Q000235:genetics; D004210:Dissection / Q000379:methods; D005260:Female; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D007723:Korea; D008297:Male; D008677:Metaphase; D009115:Muridae / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics