Post-analysis follow-up and validation of microarray experiments.
Measurement of gene-expression profiles using microarray technology is becoming increasingly popular among the biomedical research community. Although there has been great progress in this field, investigators are still confronted with a difficult question after completing their experiments: how to validate the large data sets that are generated? This review summarizes current approaches to verifying global expression results, discusses the caveats that must be considered, and describes some methods that are being developed to address outstanding problems.
Chuaqui
Rodrigo F
RF
Pathogenetics Unit, Laboratory of Pathology and Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.
Bonner
Robert F
RF
Best
Carolyn J M
CJ
Gillespie
John W
JW
Flaig
Michael J
MJ
Hewitt
Stephen M
SM
Phillips
John L
JL
Krizman
David B
DB
Tangrea
Michael A
MA
Ahram
Mamoun
M
Linehan
W Marston
WM
Knezevic
Vladimir
V
Emmert-Buck
Michael R
MR
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D005544:Forecasting; D020869:Gene Expression Profiling / Q000379:methods / Q000592:standards*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000592:standards*; D011506:Proteins / Q000235:genetics; D011786:Quality Control; D012333:RNA, Messenger / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger