title: Concentration of collagen cross-links in human dentin bears no relation to the individual age.
abstract: Lysylpyridinoline (LP) and hydroxylysylpyridinoline (HP) are collagen cross-link residues. HP is present in most tissues, whereas LP is specific for bone and dentin. Based on the current literature there are certain indications that measurement of the concentrations of HP and LP in dentin may be a valuable tool to determine the individual age. The purpose of this investigation was to assess if the concentrations of LP and HP in dentin increase during lifetime. We have investigated 173 molars from 173 individuals (2 through 78 years of age, 31 primary and 142 secondary teeth) in the course of the present study. Levels of LP and HP were measured by HPLC and fluorescence detection. The results show that dentinal concentrations of HP and LP did not increase with age and varied between individuals of the same age and that determination of dentinal concentrations of HP and LP cannot be used to determine the individual age.
authors.affiliation: Department of Oral and Maxillofacial Surgery, University of Kiel, Arnold-Hellerstrasse 16, 24105 Kiel, Germany.
authors.forename: Y
authors.initials: Y
authors.lastname: Açil
authors.forename: I N G
authors.initials: IN
authors.lastname: Springer
authors.forename: J G
authors.initials: JG
authors.lastname: Prasse
authors.forename: J
authors.initials: J
authors.lastname: Hedderich
authors.forename: S
authors.initials: S
authors.lastname: Jepsen
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000596:Amino Acids / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000032:analysis*; D003432:Cross-Linking Reagents / Q000032:analysis; D003804:Dentin / Q000737:chemistry*; D005553:Forensic Dentistry; D006801:Humans; D008875:Middle Aged; D008963:Molar / Q000737:chemistry
chemical_list: D000596:Amino Acids; D015415:Biomarkers; D003432:Cross-Linking Reagents; C015484:pyridinoline; C036020:deoxypyridinoline; D003094:Collagen