Cholesterol oxidation using hollow fiber dialyzer immobilized with cholesterol oxidase: effect of storage and reuse.
Cholesterol oxidase (COD) was covalently bonded onto the surface of polyacrylonitrile (PAN) hollow fiber via glutaraldehyde. The optimum conditions were found to be at pH 7, -20 degrees C and in the dry state. Immobilized COD retained relative activity above 42% after a 30-day period, when storing at 25 degrees C. After reusing for 30 times, the activity of dry-stored immobilized COD retained 58% of activity. By rinsing with PBS, the decrease in the activity can be greatly reduced. At a flow speed of 1.09cm/s, the immobilized COD can decrease the concentration of cholesterol by about 40% in a 4-h run.
Lin
Chi-Chang
CC
Department of Polymer Engineering, Hollow Fiber Laboratory, National Taiwan University of Science and Technology, Taipei 10672, Taiwan.
Yang
Ming-Chien
MC
D002784:Cholesterol / Q000378:metabolism*; D002789:Cholesterol Oxidase / Q000378:metabolism*; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D006435:Renal Dialysis / Q000295:instrumentation; D013696:Temperature
D004800:Enzymes, Immobilized; D002784:Cholesterol; D002789:Cholesterol Oxidase