Transcriptional response of soybean suspension-cultured cells induced by Nod factors obtained from Bradyrhizobium japonicum USDA110.
Genes responding to Nod factors were picked up by the application of a differential display method for soybean suspension-cultured cells. Forty-five cDNA fragments derived from such genes were detected. Seven fragments (ssc1-ssc7) were successfully cloned. The putative product of genes corresponding to ssc1 was estimated to be a disease-resistance protein relating to the induction of the plant defense response against pathogens, and that corresponding to ssc7 was a sucrose transporter. Amino acid sequences deduced from full-length cDNA corresponding to ssc2 and ssc4 were investigated, and it was shown that these polypeptides were equipped with a leucine zipper motif and with phosphorylation sites that were targeted by tyrosin kinase and cAMP-dependent protein kinase, respectively. In a differential display experiment, the transcriptional levels of three genes corresponding to ssc2, ssc3 and ssc5 were estimated to be up-regulated at 6 h after initiation of the treatment and the remaining four were estimated to be down-regulated. However, transcription of the genes corresponding to all ssc was clearly repressed within 2 h after initiation of the treatment. Five of them were restored to their transcriptional level 6 h after initiation of the treatment, although the others were repressed throughout the experimental period.
Hakoyama
Tsuneo
T
Faculty of Agriculture, Tokyo University of Agriculture and Technology, Saiwaicho 3-5-8, Fuchu, Tokyo 183-8509 Japan. hakoyama@cc.tuat.ac.jp
Yokoyama
Tadashi
T
Kouchi
Hiroshi
H
Tsuchiya
Ken-ichi
K
Kaku
Hisatoshi
H
Arima
Yasuhiro
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007113:Immunity, Innate / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D026901:Membrane Transport Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D030262:Soybean Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D013025:Glycine max / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D013559:Symbiosis / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*
D018076:DNA, Complementary; D008070:Lipopolysaccharides; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D030262:Soybean Proteins; C078729:sucrose transport protein, plant; C091899:Nod factor V, Bradyrhizobium japonicum