title: Induction of differentiation rescues HL-60 cells from Rana catesbeiana ribonuclease-induced cell death.
abstract: Rana catesbeiana ribonuclease (RC-RNase) exerted strong anti-tumor activity and its cytotoxicity was shown to correlate with differentiation stages of three different hepatoma cell lines. In this study, we demonstrate different RC-RNase cytotoxicity in undifferentiated HL-60 cells and in those that had been induced to differentiate by retinoic acid or dimethylsulfoxide. RC-RNase showed cytotoxicity in undifferentiated HL-60 cells, but not in HL-60 cells undergoing terminal differentiation. Furthermore, the caspase-9/caspase-3 pathway was activated when RC-RNase induced death in undifferentiated HL-60 cells and induction of differentiation led to a reversal of the caspase activation pathway.
authors.affiliation: Graduate Institute of Life Sciences, National Defense Medical Center, 114, Taipei, Taiwan.
authors.forename: Chyou Wei
authors.initials: CW
authors.lastname: Wei
authors.forename: Chih Chi Andrew
authors.initials: CC
authors.lastname: Hu
authors.forename: Chih Hang Anthony
authors.initials: CH
authors.lastname: Tang
authors.forename: Ming Chieh
authors.initials: MC
authors.lastname: Lee
authors.forename: Jaang Jiun
authors.initials: JJ
authors.lastname: Wang
mesh_terms: D029845:Amphibian Proteins / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western; D053148:Caspase 3; D053453:Caspase 9; D020169:Caspases / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects*; D002454:Cell Differentiation; D004722:Endoribonucleases / Q000494:pharmacology*; D004789:Enzyme Activation; D018922:HL-60 Cells; D006801:Humans; D011892:Rana catesbeiana
chemical_list: D029845:Amphibian Proteins; D004722:Endoribonucleases; C094067:pyrimidine-guanine sequence-specific ribonuclease, Rana catesbeiana; C505407:CASP3 protein, human; C506433:CASP9 protein, human; D053148:Caspase 3; D053453:Caspase 9; D020169:Caspases