A rat monoclonal antibody that catalyses the hydrolysis of a nitrophenyl-beta-lactam.
We report the first example of a monoclonal antibody-catalysed hydrolysis of a beta-lactam where the antibodies were generated by a simple transition-state analogue. A rat monoclonal antibody (1/91c/4d/26) generated by using an acyclic 4-nitrophenylphosphate immunogen catalysed the hydrolysis of corresponding 4-nitrophenyl carbonates but, more importantly, also catalysed the hydrolysis of N-(4-nitrophenyl)-azetidinone at pH 8 with k(cat)=8.7 x 10(-6)s(-1) and K(M)=35 microM. This is the first example of a rat monoclonal catalytic antibody.
Ostler
Elizabeth L
EL
School of Pharmacy and Biomolecular Sciences, University of Brighton, Cockcroft Building, Moulsecoomb, BN2 4GJ, Brighton, UK.
Dean
Christopher J
CJ
Barber
Nicola
N
Valeri
Maurizio
M
James
Stuart
S
Resmini
Marina
M
Boucher
Guillaume
G
Romanov
Nickolas
N
Brocklehurst
Keith
K
Gallacher
Gerard
G
D000818:Animals; D017151:Antibodies, Catalytic / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002384:Catalysis; D006868:Hydrolysis; D007700:Kinetics; D009596:Nitrophenols / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D047090:beta-Lactams / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
D017151:Antibodies, Catalytic; D000911:Antibodies, Monoclonal; D009596:Nitrophenols; D047090:beta-Lactams; C024719:4-nitrophenyl