title: The kinetics of reversal of pre-sickled erythrocytes by the aqueous extract of Cajanus cajan seeds.
abstract: The aqueous methanol extract (3:1, v/v) of the seeds of Cajanus cajan was investigated for antisickling properties. The extract possessed significant antisickling activity and was found to be concentration-dependent. The kinetics of reversal of presickled erythrocyte (HbSS) cells using the extract at 0.5 mg/mL, 1.0 mg/mL, 1.5 mg/mL, 2.0 mg/mL and 2.5 mg/mL showed first-order kinetics with rate constants of 5.833 x 10(-3) min(-1), 6.143 x 10(-3) min(-1), 5.957 x 10(-3) min(-1) 6.00 x 10(-3) min(-1) and 6.046 x 10(-3) min(-1), respectively, and with an average half-life 115.6 min. Phytochemical screening of the extract revealed the presence of free amino acids, phenolic compounds, tannins, globulins and saponins. These results show that the extract has potential use in the management of painful episodes experienced by sickle cell patients.
authors.affiliation: Department of Pharmaceutical Chemistry, University of Jos, PMB 2084, Jos, Nigeria.
authors.forename: Johnson
authors.initials: J
authors.lastname: Ogoda Onah
authors.forename: Paul I
authors.initials: PI
authors.lastname: Akubue
authors.forename: George B
authors.initials: GB
authors.lastname: Okide
mesh_terms: D000293:Adolescent; D000755:Anemia, Sickle Cell / Q000188:drug therapy; D000986:Antisickling Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D036561:Cajanus; D002648:Child; D004305:Dose-Response Relationship, Drug; D004913:Erythrocytes, Abnormal / Q000187:drug effects*; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D012639:Seeds
chemical_list: D000986:Antisickling Agents; D010936:Plant Extracts