An improved method for acetaldehyde determination in blood by high-performance liquid chromatography and solid-phase extraction.
This study reports on an improved method for acetaldehyde (ACH) determination in blood by high-performance liquid chromatography (HPLC). In the case of HPLC analysis, ACH is generally converted to derivatives for ultraviolet detection (for example 2,4-dinitrophenylhydrazine [DNPH] derivative). Nevertheless, elevation of the background during protein precipitation, hydrazone synthesis, or both frequently results in a serious loss of accuracy and precision of the analysis. The method in this study is developed to minimize the increase in nonspecific ACH-DNPH with a view to optimize mainly the synthetic condition of ACH-DNPH. The background is decreased dramatically by gentle deproteination, optimization of the DNPH amount and reaction pH, and reversed-phase solid extraction for the elimination of excess DNPH reagent. The standard curves show good linearity between 0 and 100 microM and minimal background is observed, indicating that the method is useful for monitoring the ACH concentration in blood.
Kozutsumi
Daisuke
D
Pharmaceuticals Development Department, Meiji Dairies Corporation, Odawara, Kanagawa, Japan. daisuke_kozutsumi@meiji-milk.com
Arita
Masayo
M
Kawashima
Akihiro
A
Adachi
Mami
M
Takami
Masao
M
D000079:Acetaldehyde / Q000097:blood*; D000925:Anticoagulants / Q000097:blood; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000431:Ethanol / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000925:Anticoagulants; D002021:Buffers; D000431:Ethanol; D000079:Acetaldehyde