title: Laccase stabilization by covalent binding immobilization on activated polyvinyl alcohol carrier.
abstract: OBJECTIVE
  Attempts were made to immobilize laccase from Panus conchatus.
  
  
  RESULTS
  The laccase was immobilized on carboxylated polyvinyl alcohol (PVA) activated by N-hydroxysuccinimide (N-HSI) in aqueous solution at different pHs, temperatures, and with different reaction times. An optimum condition for laccase immobilization is at pH 3.2, 40 degrees C and 12 h, respectively. Immobilization of laccase increased optimal pH for reaction with 2, 2'-azinobis (3-ethylbenzthiazoline-6-solfonate) (ABTS) and pH stability. Immobilized laccase proved to be reacted consecutively 17 times with only a 50% decrease on activity and be used in removal of 2,4,6-trichlorophenol (TCP).
  
  
  CONCLUSIONS
  It was possible to immobilize the laccase on carboxylated polyvinyl alcohol by activation with N-hydroxysuccinimide in HAc-NaAc buffer. The immobilized laccase is both stable and reusable. SIGNIFICANCE IMPACT OF THE STUDY: The results indicate that this immobilized laccase can be used in the removal of poisonous effluent from pulp bleaching mills.
authors.affiliation: Laboratory of Cellulose and Lignocellulosics Chemistry, Guangzhou Institute of Chemistry, Chinese Academy of Sciences, Guanzhou, PR, China.
authors.forename: Diao
authors.initials: D
authors.lastname: Yinghui
authors.forename: Wang
authors.initials: W
authors.lastname: Qiuling
authors.forename: Fu
authors.initials: F
authors.lastname: Shiyu
mesh_terms: D001487:Basidiomycota / Q000201:enzymology*; D002733:Chlorophenols / Q000378:metabolism; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D007220:Industrial Waste; D042845:Laccase; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism*; D010209:Paper; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D014865:Waste Disposal, Fluid / Q000379:methods
chemical_list: D002733:Chlorophenols; D004800:Enzymes, Immobilized; D007220:Industrial Waste; D011142:Polyvinyl Alcohol; D010088:Oxidoreductases; D042845:Laccase; C024564:2,4,6-trichlorophenol