Comparative chemical attributes of native North American hop, Humulus lupulus var. lupuloides E. Small.
The genetic diversity of 159 representative genotypes of native hop (Humulus lupulus var. lupuloides E. Small, Cannabaceae) from 34 selected populations was assessed by relative magnitudes and ranges of alpha acids (AA), beta acids (BA), and the cohumulone (CoH) component of alpha acids, with reference to temporal changes between 1989-1990 and 2001, and to the same attributes in American and European hop cultivars, principally H. lupulus var. lupulus L. Chemical profiles of these genotypes were generated by high pressure liquid chromatography (HPLC) of methanol extracts from their processed samples (cones). The alpha ratio (AR, alpha acids / alpha+beta acids) measured the degree to which alpha acids predominated in cone extracts. Synchronous ranges of AR and CoH were also selected for graphic portrayals of native hop genotypic diversity. Cones sampled and analyzed from eight populations that were accessible in both 1989 and 2001 were distinct in chemical attributes, indicating a succession of genotypes, and suggesting temporal cycling of H. lupulus var.lupuloides germplasm. The principal distinctions between the two sub-species were a markedly higher proportion of CoH (38-88% vs. 19-41%) in alpha acids of H. l. var. lupuloides, and generally higher concentrations of AA in cultivars of both American and European commercial hop cultivars, predominantly H. lupulus var. lupulus. All of the 159 native hop genotypes also contained detectable levels of xanthohumol and xanthogalenol, prenylflavonoids recently reported to have mammalian anti-cancer activity. Some native genotypes had previously exhibited natural repellence of insect and mite pests; thus H. lupulus var. lupuloides germplasm offers a diverse resource of underutilized and yet undefined biochemicals.
Hampton
Richard
R
Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR 97331, USA. hamporc@fmtc.com
Nickerson
Gail
G
Whitney
Peggy
P
Haunold
Alfred
A
D002264:Carboxylic Acids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003512:Cyclohexanones / Q000032:analysis / Q000378:metabolism; D003627:Data Interpretation, Statistical; D005060:Europe; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D040961:Genetic Drift; D014644:Genetic Variation; D005838:Genotype; D027582:Humulus / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D009656:North America; D011153:Population / Q000235:genetics; D012815:Signal Processing, Computer-Assisted; D013997:Time Factors
D002264:Carboxylic Acids; D003512:Cyclohexanones; D005419:Flavonoids; C442487:cohumulone