Protein fingerprinting of Saccharomyces isolates with therapeutic relevance using one- and two-dimensional electrophoresis.
In therapeutic products and preparations Saccharomyces cerevisiae is used because of its nutritive properties. Moreover, so called Saccharomyces boulardii yeasts are used in the prevention and treatment of several types of diarrhea. Taxonomically however, S. boulardii is not accepted as a distinct species. The protein fingerprint obtained after sodium dodecyl sulfate-polyacrylamide gel electrophoresis was identical for all isolates and therefore confirmed the designation of S. boulardii to the species S. cerevisiae. In contrast, using native polyacrylamide gel electrophoresis, 12 different protein fingerprints were detected, and allowed grouping of the product isolates. The spot patterns obtained by two-dimensional electrophoresis revealed a large degree of resemblance, however, small qualitative expression differences could be detected as well. Firstly, a spot having an isoelectric point of approximately 6 and 30 kDa could not be detected in S. boulardii yeasts. Secondly, nine different formations of spots occurred in the region around 16 kDa and pH 6. Therefore, on the one hand, it could be demonstrated that all of the product isolates belong to the same species, and on the other hand, it was possible to extensively subdivide the strains. In particular, two-dimensional electrophoresis allowed clustering of so called S. boulardii strains within the species S. cerevisiae.
Mitterdorfer
Gabriele
G
Department of Dairy Research and Bacteriology, University of Agricultural Sciences, Vienna, Austria.
Mayer
Helmut K
HK
Kneifel
Wolfgang
W
Viernstein
Helmut
H
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D008970:Molecular Weight; D010449:Peptide Mapping / Q000379:methods*; D012440:Saccharomyces / Q000737:chemistry / Q000145:classification; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000145:classification; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D013045:Species Specificity
D029701:Saccharomyces cerevisiae Proteins