title: Plasticity of electrical pacemaking by interstitial cells of Cajal and gastric dysrhythmias in W/W mutant mice.
abstract: OBJECTIVE
  Interstitial cells of Cajal (ICC) generate and propagate slow waves in the stomach. Gastric peristalsis depends on a proximal-to-distal gradient in slow wave frequency. We tested whether the gastric frequency gradient was an intrinsic property of ICC and whether dysrhythmias result from disruptions of ICC networks.
  
  
  METHODS
  We studied wild-type (WT) and W/W(V) mice, which have only myenteric (pacemaker) ICC in the stomach. ICC distributions were analyzed by Kit immunofluorescence. Pacemaking in tissues was studied by intracellular electrophysiologic recording and in cultured ICC by monitoring mitochondrial [Ca(2+)] oscillations with rhod-2 fluorescence or membrane potential with DiBAC(4)(3) fluorescence.
  
  
  RESULTS
  Slow wave frequencies were constant throughout WT gastric muscle sheets containing corpus and antrum. Separating the antrum from the corpus caused a significant drop in antral slow wave frequency. ICC from WT antrums also displayed significantly slower pacemaker frequencies than corpus ICC, but the corpus pacemaker frequency dominated in cocultures of corpus and antrum ICC. Myenteric ICC networks were reduced in W/W(V) mice, particularly in the corpus. In W/W(V) mice, separating the antrum from the corpus failed to reduce antral slow wave frequency. Antral pacemaker frequency in ICC from W/W(V) stomachs was the same as in corpus ICC.
  
  
  CONCLUSIONS
  The proximal-to-distal slow wave frequency gradient and entrainment of distal electrical activity by proximal pacemakers are fundamental properties of gastric ICC. Chronic depletion of ICC networks disrupts the proximal-to-distal frequency gradient, and emergence of ectopic pacemakers in the antrum may be caused by "reprogramming" of the ICC pacemaker apparatus.
authors.affiliation: Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, 89557, USA.
authors.forename: Tamás
authors.initials: T
authors.lastname: Ordög
authors.forename: Marjolaine
authors.initials: M
authors.lastname: Baldo
authors.forename: Reka
authors.initials: R
authors.lastname: Danko
authors.forename: Kenton M
authors.initials: KM
authors.lastname: Sanders
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004594:Electrophysiology; D051379:Mice; D008817:Mice, Mutant Strains; D009197:Myenteric Plexus / Q000473:pathology / Q000503:physiopathology*; D009415:Nerve Net / Q000503:physiopathology; D009473:Neuronal Plasticity; D010507:Periodicity; D013272:Stomach Diseases / Q000473:pathology / Q000503:physiopathology*