Identification of the CAB2/hCOS16 gene required for the repair of DNA double-strand breaks on a core amplified region of the 17q12 locus in breast and gastric cancers.
We previously reported that CAB1 and c-ERBB-2 genes were found to be located in a core amplified region of the 17q12 locus, which is frequently amplified in various cancers. During identification of this core region, CAB2, a human homologue of the yeast COS16 required for the repair of DNA double-strand breaks was cloned. Autofluorescence analysis of cells transfected with its GFP fusion protein demonstrated that CAB2 translocates into vesicles, suggesting that overexpression of CAB2 may decrease intercellular Mn2+ by accumulating it in the vesicles, in the same way as yeast COS16. This is the first report identifying all of the genes on the core amplified region of the 17q12 locus in breast and gastric cancers.
Nezu
Masahiko
M
Genetics Division, National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045, Japan.
Nishigaki
Michiko
M
Ishizuka
Tomoki
T
Kuwahara
Yoshitaka
Y
Tanabe
Chikako
C
Aoyagi
Kazuhiko
K
Sakamoto
Hiromi
H
Saito
Yasushi
Y
Yoshida
Teruhiko
T
Sasaki
Hiroki
H
Terada
Masaaki
M
D000595:Amino Acid Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009857:Oncogenes; D013274:Stomach Neoplasms / Q000235:genetics*