Fundamentals of experimental design for cDNA microarrays.
Microarray technology is now widely available and is being applied to address increasingly complex scientific questions. Consequently, there is a greater demand for statistical assessment of the conclusions drawn from microarray experiments. This review discusses fundamental issues of how to design an experiment to ensure that the resulting data are amenable to statistical analysis. The discussion focuses on two-color spotted cDNA microarrays, but many of the same issues apply to single-color gene-expression assays as well.
Churchill
Gary A
GA
The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA. garyc@jax.org
D000818:Animals; D018076:DNA, Complementary / Q000032:analysis*; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D051379:Mice; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012015:Reference Standards; D015203:Reproducibility of Results; D012107:Research Design; D013223:Statistics as Topic
D018076:DNA, Complementary