Rapid and noninvasive diagnosis of the presence and severity of coronary heart disease using 1H-NMR-based metabonomics.
Although a wide range of risk factors for coronary heart disease have been identified from population studies, these measures, singly or in combination, are insufficiently powerful to provide a reliable, noninvasive diagnosis of the presence of coronary heart disease. Here we show that pattern-recognition techniques applied to proton nuclear magnetic resonance (1H-NMR) spectra of human serum can correctly diagnose not only the presence, but also the severity, of coronary heart disease. Application of supervised partial least squares-discriminant analysis to orthogonal signal-corrected data sets allows >90% of subjects with stenosis of all three major coronary vessels to be distinguished from subjects with angiographically normal coronary arteries, with a specificity of >90%. Our studies show for the first time a technique capable of providing an accurate, noninvasive and rapid diagnosis of coronary heart disease that can be used clinically, either in population screening or to allow effective targeting of treatments such as statins.
Brindle
Joanne T
JT
Biological Chemistry, Biomedical Sciences Division, Faculty of Medicine, Imperial College of Science, Technology and Medicine, Sir Alexander Fleming Building, Exhibition Road, South Kensington, London, UK.
Antti
Henrik
H
Holmes
Elaine
E
Tranter
George
G
Nicholson
Jeremy K
JK
Bethell
Hugh W L
HW
Clarke
Sarah
S
Schofield
Peter M
PM
McKilligin
Elaine
E
Mosedale
David E
DE
Grainger
David J
DJ
D003324:Coronary Artery Disease / Q000175:diagnosis; D003327:Coronary Disease / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D016018:Least-Squares Analysis; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*