Effect of stress hormones on the expression of fibrinogen-binding receptors in platelets.
Acute coagulopathy is a common clinical complication after trauma, and contributes to posttraumatic multiple organ failure. The phenomenon may be due to the effect of stress hormones on platelet adhesion molecule expression after trauma. Catecholamine levels correlate with injury severity scores and changes of L-selectin expression on leucocytes, whilst adrenaline (ADR) (epinephrine) alone also activates platelets. This study thus investigates the effects of ADR and noradrenaline (NOR) (norepinephrine) on platelets, at doses similar to those found in the plasma of normal and trauma subjects. Blood was taken from 19 healthy subjects and placed in tubes containing sodium citrate. Anti-platelet-bound fibrinogen monoclonal antibody was used to identify the activated platelets while anti-CD41 was used to identify platelets with and without activation. Five increasing concentrations of ADR and NOR (1, 3, 5, 10, 30 nmol/l) as well as one negative control (0.9% normal saline) and one positive control (10 micromol/l adenosine diphosphate/ADP) were prepared for the stimulation. A whole blood protocol was used in order to minimize any activation artefacts, which might be created by centrifugation. The percentage of platelets expressing fibrinogen receptors increased significantly with ADR and NOR even at the lowest dose (1 nmol/l) and continued to increase in a dose-dependent manner. Although the effect of ADR was greater than NOR in stimulating platelets to express fibrinogen receptors, the average number of fibrinogen receptors on each platelet was constant. ADR and NOR activated platelets to express fibrinogen receptors at doses that are similar to those found in the plasma of trauma patients.
Lam
Nicole Y-L
NY
Accident and Emergency Medicine Academic Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong.
Rainer
Timothy H
TH
Ng
Margaret H-L
MH
Leung
Yonna
Y
Cocks
Robert A
RA
D000328:Adult; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D004837:Epinephrine / Q000494:pharmacology*; D005260:Female; D005340:Fibrinogen / Q000187:drug effects* / Q000502:physiology; D005434:Flow Cytometry; D006801:Humans; D016014:Linear Models; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology*; D015539:Platelet Activation / Q000187:drug effects; D013566:Sympathomimetics / Q000494:pharmacology*; D014947:Wounds and Injuries / Q000097:blood
D013566:Sympathomimetics; D005340:Fibrinogen; D009638:Norepinephrine; D004837:Epinephrine