title: Cryopreservation of Citrus madurensis embryonic axes encapsulation-dehydration.
abstract: In this paper, we demonstrate that C. madurensis embryonic axes can withstand cryopreservation using the encapsulation-dehydration technique. Up to 57.5 % survival was achieved using a standard encapsulation-dehydration protocol, which included pregrowth of encapsulated axes for 16 h in medium containing 0.8 M sucrose + 1 M glycerol, desiccation of beads to around 30 % moisture content (fresh weight basis) followed by rapid freezing. A slightly higher survival percentage (65 %) was obtained using a modified encapsulation-dehydration protocol, which included pretreatment of axes with 2 M glycerol + 0.6 M sucrose for 1 h, concomitantly with their encapsulation in 3 % calcium alginate beads, followed by desiccation of the beads to around 30 % moisture content.
authors.affiliation: International Plant Genetic Resources Institute (IPGRI), Regional Office for Asia, Pacific and Oceania, P.O. Box 236, UPM Post Office, 43400 Serdang, Selangor Darul Ehsan, Malaysia. e.cho@rda.go.kr.
authors.forename: Eun Gi
authors.initials: EG
authors.lastname: Cho
authors.forename: Y L
authors.initials: YL
authors.lastname: Hor
authors.forename: Haeng Hoon
authors.initials: HH
authors.lastname: Kim
authors.forename: V Ramanatha
authors.initials: VR
authors.lastname: Rao
authors.forename: Florent
authors.initials: F
authors.lastname: Engelmann
mesh_terms: D002957:Citrus / Q000196:embryology*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D046508:Culture Techniques / Q000379:methods; D003890:Desiccation / Q000379:methods*; D012639:Seeds / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development*; D015996:Survival Rate; D013997:Time Factors; D014867:Water / Q000032:analysis
chemical_list: D003451:Cryoprotective Agents; D014867:Water