Probing the structural dynamics of nucleic acids by quantitative time-resolved and equilibrium hydroxyl radical "footprinting".
For many years, hydroxyl radical footprinting has been an insightful probe of the solvent accessibility of local regions of nucleic acid structure. Recently, quantitative applications of this technique have been developed that allow time-resolved and equilibrium analysis of transitions involving nucleic acid ligand binding and conformation change to be analyzed incisively.
Brenowitz
Michael
M
Department of Biochemistry, Center for Synchrotron Biosciences, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA. brenowit@aecom.yu.edu
Chance
Mark R
MR
Dhavan
Gauri
G
Takamoto
Keiji
K
D004247:DNA / Q000737:chemistry*; D018983:DNA Footprinting / Q000379:methods*; D017665:Hydroxyl Radical; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*
D009696:Nucleic Acids; D017665:Hydroxyl Radical; D012313:RNA; D004247:DNA