Regulation of the manganese-containing superoxide dismutase gene from fission yeast.
The manganese superoxide dismutase (MnSOD) is a mitochondrial enzyme that dismutates a potentially toxic superoxide radical into hydrogen peroxide and dioxygen. To study the regulation of the Schizosaccharomyces pombe MnSOD gene, the 943 bp upstream region was fused into the promoterless beta-galactosidase gene of the shuttle vector YEp357, which resulted in the fusion plasmid pMS14. Restriction mapping and nucleotide sequencing confirmed its construction. The synthesis of beta-galactosidase from the fusion plasmid was induced by aluminum chloride, menadione, cadmium chloride, manganese chloride, and hydrogen peroxide. It was also induced by NO-generating S-nitroso-N-acetylpenicillamine (SNAP). However, cupric chloride and zinc chloride did not affect the synthesis of beta-galactosidase from the fusion plasmid. The beta-galactosidase synthesis appeared to be independent of the Pap1 protein. These results suggest that some metals, oxidative stress, and nitric oxide regulate the S. pombe MnSOD gene.
Jung
Haeng-Im
HI
Division of Life Sciences, Kangwon National University, Chuncheon, Korea.
Lee
Yuk-Young
YY
Lim
Hye-Won
HW
Ahn
Ki-Sup
KS
Park
Eun-Hee
EH
Lim
Chang-Jin
CJ
D020121:5' Untranslated Regions / Q000235:genetics; D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D006861:Hydrogen Peroxide / Q000378:metabolism; D008345:Manganese / Q000378:metabolism*; D008670:Metals / Q000378:metabolism; D000073718:Pancreatitis-Associated Proteins; D010396:Penicillamine / Q000031:analogs & derivatives* / Q000378:metabolism; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism*; D013696:Temperature; D024483:Vitamin K 3 / Q000378:metabolism
D020121:5' Untranslated Regions; D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D008670:Metals; D000073718:Pancreatitis-Associated Proteins; C105096:Pap1 protein, S pombe; C000612141:REG3A protein, human; C110881:S-nitro-N-acetylpenicillamine; D029702:Schizosaccharomyces pombe Proteins; D008345:Manganese; D024483:Vitamin K 3; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D010396:Penicillamine