Biochemical identification of A-minor motifs within RNA tertiary structure by interference analysis.
A-minor motifs are the most common tertiary structural elements in RNA helix packing. Biochemical identification of these interactions is now feasible using interference mapping analysis with the adenosine analogues 2'-deoxyadenosine and 3-deaza-adenosine. This approach was used to demonstrate that A-minor motifs mediate helix packing interactions that are important for 5'-splice site selection in the group I intron. By analysing the interference pattern of several analogues it is possible to identify and distinguish the four variants of the A-minor motif.
Strobel
S A
SA
Department of Molecular Biophysics and Biochemistry, Department of Chemistry, 260 Whitney Avenue, Yale University, New Haven, CT 06520-8114, USA. strobel@mail.csb.yale.edu
D001665:Binding Sites / Q000235:genetics; D005091:Exons; D007438:Introns; D008956:Models, Chemical; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D016337:RNA, Catalytic / Q000737:chemistry* / Q000235:genetics
D016337:RNA, Catalytic; D012313:RNA