title: Factors affecting ascorbic acid (AsA) biosynthesis in chickens. II. Effect of dietary AsA and strain of chicken.
abstract: The present study examined the effect of supplemental ascorbic acid (AsA) and ascertained if genotype is a determinant of biosynthesis and the response of strains to dietary AsA. Slow- (Ottawa Meat Control; OMC) and fast-growing (Peterson Enhanced x Hubbard; PEH) chicks were fed 1000 mg/kg AsA from 1 to 10 weeks of age. The activity of l-gulonolactone oxidase (GLO) was used to measure biosynthesis and estimated synthetic capacity (ESC) computed. Body weight was not affected by diets and relative kidney weight decreased with age. In 1 week, dietary AsA increased plasma AsA and inhibited GLO activity with a greater reduction in OMC birds. At 10 weeks, GLO activity was depressed almost uniformly in both strains. Strain by age and diet by age interactions were detected for GLO activity and ESC with significantly greater decline in PEH birds and birds fed supplemental AsA. The results demonstrated that dietary AsA inhibited biosynthesis in meat type chickens and the response at 10 weeks was not influenced by growth rate; and the age dependent decline in biosynthesis was more pronounced in the commercial PEH birds. The result suggests that such strains may be compromised in some situations. Research using multiple dietary levels of AsA, commercial strains, and defined stressors is warranted.
authors.affiliation: Department of Animal and Veterinary Sciences, Clemson University, SC 29634, USA.
authors.forename: C L
authors.initials: CL
authors.lastname: Hooper
authors.forename: D V
authors.initials: DV
authors.lastname: Maurice
authors.forename: S F
authors.initials: SF
authors.lastname: Lightsey
authors.forename: J E
authors.initials: JE
authors.lastname: Toler
mesh_terms: D000367:Age Factors; D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage* / Q000096:biosynthesis*; D001835:Body Weight / Q000187:drug effects; D002645:Chickens / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D005838:Genotype; D007668:Kidney / Q000254:growth & development; D050544:L-Gulonolactone Oxidase; D008099:Liver / Q000201:enzymology; D009929:Organ Size / Q000187:drug effects; D011897:Random Allocation; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D013401:Sugar Alcohol Dehydrogenases; D050544:L-Gulonolactone Oxidase; D001205:Ascorbic Acid