Expression of hpttg proto-oncogene in lymphoid neoplasias.
Pituitary tumor-transforming gene (pttg) is a distinct proto-oncogene which is expressed in certain normal tissues with high proliferation rate and in a variety of tumors. PTTG is the vertebrate analog of yeast securins Pds1 and Cut2 with a key role in the regulation of sister chromatid separation during mitosis. Impairment of PTTG regulated functions is expected to lead to chromosomal instability and aneuploidy. Human pttg (hpttg) is abundantly expressed in Jurkat T lymphoblastic lymphoma cells but not in normal peripheral blood leukocytes. To obtain additional data on the potential role of hpttg in lymphomagenesis we selected 150 cases of lymphoid tumors for the assessment of hpttg expression in tumor tissues. Immunohistochemical studies on formalin-fixed, paraffin-embedded tissues revealed hPTTG in 38.8% of B-cell lymphomas, 70.2% of T-cell lymphomas, and 73.1% of Hodgkin's lymphomas. Among B-cell lymphomas, the most frequently immunostained tumors were plasma cell tumors, diffuse large cell lymphomas, and follicle center cell lymphomas. In Hodgkin's disease, immunoreactivity was mainly noted in Reed-Sternberg cells. In conclusion, the frequent overexpression of hpttg in many histological subtypes of lymphoma suggests the involvement of this proto-oncogene in lymphomagenesis.
Sáez
Carmen
C
Department of Pathology, Hospital Universitario Virgen del Rocío, Seville, 41013 Spain.
Pereda
Teresa
T
Borrero
Juan J
JJ
Espina
Agueda
A
Romero
Francisco
F
Tortolero
María
M
Pintor-Toro
José A
JA
Segura
Dolores I
DI
Japón
Miguel A
MA
D015152:Blotting, Northern; D015972:Gene Expression Regulation, Neoplastic; D006689:Hodgkin Disease / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D008223:Lymphoma / Q000737:chemistry* / Q000235:genetics; D016393:Lymphoma, B-Cell / Q000737:chemistry / Q000235:genetics; D016403:Lymphoma, Large B-Cell, Diffuse / Q000737:chemistry / Q000235:genetics; D016399:Lymphoma, T-Cell / Q000737:chemistry / Q000235:genetics; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000032:analysis / Q000096:biosynthesis; D016539:Reed-Sternberg Cells / Q000737:chemistry; D064249:Securin
C000715470:MAS1 protein, human; D009363:Neoplasm Proteins; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D064249:Securin; C578002:pituitary tumor-transforming protein 1, human