title: cDNA microarray analysis of changes in gene expression induced by neuronal hypoxia in vitro.
abstract: We used cDNA microarray gene expression profiling to characterize the transcriptional response to exposure of cultured mouse cerebral cortical neurons to hypoxia for 24 hr. Of 11,200 genes examined, 1,405 (12.5%) were induced or repressed at least 1.5-fold, whereas 26 known genes were induced and 20 known genes were repressed at least 2.5-fold. The most strongly induced genes included genes coding for endoplasmic reticulum proteins (Ero1L/Giig11, Sac1p, Ddit3/Gadd153), proteins involved in ubiquitination (Arih2, P4hb), proteins induced by hypoxia in non-neuronal systems (Gpi1, Aldo1, Anxa2, Hig1), and proteins that might promote cell death (Gas5, Egr1, Ndr1, Vdac2). These findings reinforce the importance of endoplasmic reticulum-based mechanisms and of protein-ubiquitination pathways in the neuronal response to hypoxia.
authors.affiliation: Buck Institute for Age Research, Novato, CA 94945, USA.
authors.forename: K
authors.initials: K
authors.lastname: Jin
authors.forename: X O
authors.initials: XO
authors.lastname: Mao
authors.forename: M W
authors.initials: MW
authors.lastname: Eshoo
authors.forename: G
authors.initials: G
authors.lastname: del Rio
authors.forename: R
authors.initials: R
authors.lastname: Rao
authors.forename: D
authors.initials: D
authors.lastname: Chen
authors.forename: R P
authors.initials: RP
authors.lastname: Simon
authors.forename: D A
authors.initials: DA
authors.lastname: Greenberg
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D015687:Cell Hypoxia / Q000502:physiology*; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D015870:Gene Expression; D051379:Mice; D009474:Neurons / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000378:metabolism; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D011506:Proteins; D025801:Ubiquitin