title: Terminal RNA replication elements in human parechovirus 1.
abstract: To define structural elements critical for RNA replication in human parechovirus 1 (HPeV1), a replicon with chloramphenicol acetyltransferase as a reporter gene and an infectious virus cDNA clone have been used. It was observed that there are cis-acting signals required for HPeV1 replication located within the 5'-terminal 112 nucleotides of the genome and that these include two terminal stem-loops, SL-A and SL-B, together with a pseudoknot element. Significant disruption of any of these structures impaired both RNA replication and virus growth. In view of the similarity in terminal structures to several picornaviruses, such as cardioviruses and hepatoviruses, the insights generated in this work are of wider significance for understanding picornavirus replication.
authors.affiliation: Department of Biological Sciences, University of Essex, Colchester CO4 3SQ, United Kingdom.
authors.forename: Abdolrahman S
authors.initials: AS
authors.lastname: Nateri
authors.forename: Pamela J
authors.initials: PJ
authors.lastname: Hughes
authors.forename: Glyn
authors.initials: G
authors.lastname: Stanway
mesh_terms: D020121:5' Untranslated Regions / Q000502:physiology; D001483:Base Sequence; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D030061:Parechovirus / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis* / Q000737:chemistry; D014779:Virus Replication
chemical_list: D020121:5' Untranslated Regions; D012367:RNA, Viral