Anomalous differences of light elements in determining precise binding modes of ligands to glycerol-3-phosphate dehydrogenase.
Pathogenic protozoa such as Trypanosome and Leishmania species cause tremendous suffering worldwide. Because of their dependence on glycolysis for energy, the glycolytic enzymes of these organisms, including glycerol-3-phosphate dehydrogenase (GPDH), are considered attractive drug targets. Using the adenine part of NAD as a lead compound, several 2,6-disubstituted purines were synthesized as inhibitors of Leishmania mexicana GPDH (LmGPDH). The electron densities for the inhibitor 2-bromo-6-chloro-purine bound to LmGPDH using a "conventional" wavelength around 1 A displayed a quasisymmetric shape. The anomalous signals from data collected at 1.77 A clearly indicated the positions of the halogen atoms and revealed the multiple binding modes of this inhibitor. Intriguing differences in the observed binding modes of the inhibitor between very similarly prepared crystals illustrate the possibility of crystal-to-crystal variations in protein-ligand complex structures.
Choe
Jungwoo
J
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
Suresh
Stephen
S
Wisedchaisri
Goragot
G
Kennedy
Kevin J
KJ
Gelb
Michael H
MH
Hol
Wim G J
WG
D000818:Animals; D000981:Antiprotozoal Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D001665:Binding Sites; D003460:Crystallization; D018360:Crystallography, X-Ray; D005583:Fourier Analysis; D005993:Glycerolphosphate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000737:chemistry*; D007894:Leishmania mexicana / Q000201:enzymology; D008024:Ligands; D008958:Models, Molecular; D011485:Protein Binding; D011687:Purines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology
D000981:Antiprotozoal Agents; D008024:Ligands; D011687:Purines; D005993:Glycerolphosphate Dehydrogenase