title: Rous sarcoma virus negative regulator of splicing selectively suppresses SRC mRNA splicing and promotes polyadenylation.
abstract: Retroviruses require a balance of spliced and unspliced RNA for efficient replication. Here, we examined the effect of mutations in a splicing suppressor sequence called the negative regulator of splicing (NRS), located within the gag gene of Rous sarcoma virus. While the NRS mutant viruses showed only small changes in the levels of spliced env mRNAs, they had significant increases in src mRNA levels and transformed cells more efficiently than wild-type virus. None of these mutations prevented viral replication; however, some of the mutant viruses replicated more slowly than wild-type virus. In addition, increased transcriptional readthrough of the poly(A) site in the 3' LTR was observed with the NRS mutant viruses, suggesting that the wild-type NRS sequence promotes polyadenylation.
authors.affiliation: Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
authors.forename: Christopher T
authors.initials: CT
authors.lastname: O'Sullivan
authors.forename: Tatjana S
authors.initials: TS
authors.lastname: Polony
authors.forename: Robert E
authors.initials: RE
authors.lastname: Paca
authors.forename: Karen L
authors.initials: KL
authors.lastname: Beemon
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002478:Cells, Cultured; D002642:Chick Embryo; D004742:Enhancer Elements, Genetic; D005347:Fibroblasts; D015967:Gene Expression Regulation, Viral; D015750:Genes, gag; D016391:Genes, src / Q000235:genetics*; D017354:Point Mutation; D026723:Polyadenylation; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral