Peptide washout and permeability from glyceryl monooleate buccal delivery systems.
Simultaneous evaluation of the permeation and washout of a peptide from the mucoadhesive liquid crystalline phases of glyceryl monooleate (GMO) has been investigated using a donor compartment flow-through diffusion cell. [D-Ala2, D-Leu5]enkephalin (DADLE) was incorporated into the cubic and lamellar liquid crystalline phases of GMO and applied to excised porcine buccal mucosa mounted in the donor compartment flow-through cell. Phosphate-buffered saline pH 7.4 (PBS) was pumped across the upper surface of the liquid crystalline phases to mimic salivary flow. The steady-state fluxes of DADLE and GMO from the cubic phase were significantly greater than that from the lamellar phase (P < 0.01). There was no statistical difference between the amounts of DADLE and GMO washed out from the lamellar and cubic phases (P > 0.05). The donor compartment flow-through diffusion cell was found to be a useful tool to evaluate the impact of salivary washout on mucoadhesive oral mucosal delivery systems.
Lee
Jaehwi
J
School of Pharmacy, University of London, 29/39 Brunswick Square, London WC1N 1AX, UK.
Kellaway
Ian W
IW
D000268:Adhesiveness; D000278:Administration, Buccal; D000818:Animals; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D003460:Crystallization; D004058:Diffusion; D004337:Drug Carriers; D004355:Drug Stability; D016308:Enkephalin, Leucine-2-Alanine / Q000008:administration & dosage* / Q000032:analysis / Q000493:pharmacokinetics; D005989:Glycerides; D066298:In Vitro Techniques; D008954:Models, Biological; D010539:Permeability; D012463:Saliva / Q000737:chemistry; D013552:Swine; D013997:Time Factors
D004337:Drug Carriers; D005989:Glycerides; D016308:Enkephalin, Leucine-2-Alanine; C005953:monoolein