Stem-cell leukemia: p53 deficiency mediated suppression of leukemic differentiation in C3H/He myeloid leukemia.
C3H/He mice produce myeloid leukemias after whole body irradiation of 1-3Gy as compared with non-irradiated controls that produce fewer than 1% of leukemia [Radiatiton Research 127 (1991) 146]. Thus, p53-deficient C57BL/6 strain, a malignant lymphoma prone, was crossed back into C3H/He strain. Lethally irradiated wild-type mice to which p53-deficient bone marrow cells were transplanted (transplantation assay) showed dramatic change in the propensity of leukemia of myeloid lineages, the cells lacking CD3, Thy1.2, sIgM, B220, Mac-1, Gr-1, but being positive for c-Kit and CD44. Furthermore, transplanted mice subjected to 3Gy irradiation gave rise to a faster development of leukemia and a higher frequency of double-lineage leukemias than the non-irradiated control.
Yoshida
Kazuko
K
Radiation Hazards Research Group, National Institute of Radiological Sciences, Chibashi, Japan. yosida@nirs.go.jp
Aizawa
Shiro
S
Watanabe
Keiko
K
Hirabayashi
Yoko
Y
Inoue
Tohru
T
D000208:Acute Disease; D000818:Animals; D001854:Bone Marrow Cells / Q000473:pathology; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D019070:Cell Lineage / Q000528:radiation effects; D016130:Immunophenotyping; D015994:Incidence; D007938:Leukemia; D007951:Leukemia, Myeloid / Q000209:etiology* / Q000473:pathology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency* / Q000235:genetics; D014916:Whole-Body Irradiation
D016159:Tumor Suppressor Protein p53