A recessive mutant cell line with a constitutive IkappaB kinase activity.
To search for negative regulatory components of the NF-kappaB activation pathways, we mutagenized Rat-1 fibroblasts and established a stable mutant cell line with a constitutive NF-kappaB activity. This mutant cell line, designated as TK26, showed permanently elevated I kappa B kinase (IKK) activity and a genetically recessive phenotype revealed by somatic cell hybridization between TK26 and Rat-1. Our results suggested that lack of a negative regulation of IKK could lead to permanent NF-kappaB activation. The TK26 cell line will be useful to genetically identify a component necessary for keeping the IKK complex under an inactive form in resting cells.
Chinanonwait
Netnaphis
N
Department of Molecular Virology, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, 113-8519, Tokyo, Japan.
Miura
Hideyasu
H
Yamamoto
Naoki
N
Yamaoka
Shoji
S
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015342:DNA Probes; D024202:Electrophoretic Mobility Shift Assay; D005808:Genes, Recessive; D051550:I-kappa B Kinase; D009154:Mutation; D016328:NF-kappa B / Q000378:metabolism; D010641:Phenotype; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D051381:Rats
D015342:DNA Probes; D016328:NF-kappa B; D017346:Protein Serine-Threonine Kinases; D051550:I-kappa B Kinase