title: Development of supercritical fluid extraction with a solid-phase trapping for fast estimation of toxic load of polychlorinated dibenzo-p-dioxins-dibenzofurans in sawmill soil.
abstract: A method consisting of automated supercritical fluid extraction (SFE) with simultaneous cleanup by a solid-phase trap was developed for fast analysis of polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs) in soil. SFE was optimised to replace conventional liquid-based methods in routine analyses of PCDD/PCDFs in sawmill soil contaminated by a chlorophenol formulation. PCDD/PCDFs were quantitatively extracted in 60 min using CO2 at 400 atm and 100 degrees C without a modifier. A trap containing a small amount of activated carbon mixed with Celite efficiently collected PCDD/PCDFs after SFE. After SFE co-extracted impurities were eluted out from the trap with 4 ml of hexane and PCDD/PCDFs were eluted with 10 ml of toluene. The concentrations and TCDD-equivalent of PCDD/PCDFs corresponded to the results of traditional solvent extraction method (Soxhlet) in six sawmill soils tested. The performance of the trap was maintained over a long period of time (nearly 100 extractions).
authors.affiliation: Department of Environmental Health, National Public Health Institute, P.O. Box 95, FIN-70701 Kuopio, Finland.
authors.forename: Mari
authors.initials: M
authors.lastname: Mannila
authors.forename: Jaana
authors.initials: J
authors.lastname: Koistinen
authors.forename: Terttu
authors.initials: T
authors.lastname: Vartiainen
mesh_terms: D001572:Benzofurans / Q000032:analysis*; D000072338:Dibenzofurans, Polychlorinated; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012989:Soil Pollutants / Q000032:analysis*
chemical_list: D001572:Benzofurans; D000072338:Dibenzofurans, Polychlorinated; D000072317:Polychlorinated Dibenzodioxins; D012989:Soil Pollutants