title: TREN (Tris(2-aminoethyl)amine): an effective scaffold for the assembly of triple helical collagen mimetic structures.
abstract: A new scaffold, TREN-(suc-OH)(3) where TREN is tris(2-aminoethyl)amine and suc is the succinic acid spacers, was incorporated to assemble triple helices composed of Gly-Nleu-Pro sequences (Nleu denotes N-isobutylglycine). Extensive biophysical studies which include denaturation studies, CD and NMR spectroscopy, and molecular modeling demonstrated that TREN-[suc-(Gly-Nleu-Pro)(n)-NH(2)](3) (n = 5 and 6) form stable triple helical structures in solution. A comparative analysis of TREN-assembled and KTA-assembled collagen mimetics (KTA denotes Kemp triacid, 1,3,5-trimethylcyclohexane-1,3,5-tricarboxylic acid) indicates that the flexibility of the TREN scaffold is superior to the KTA scaffold in inducing triple helicity. This effect most likely arises from the flexibility of the TREN scaffold which allows the three peptide chains to adjust their register for a tighter triple helical packing.
authors.affiliation: Department of Chemistry and Biochemistry, University of California-San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0343, USA.
authors.forename: Juliann
authors.initials: J
authors.lastname: Kwak
authors.forename: Antonia
authors.initials: A
authors.lastname: De Capua
authors.forename: Elsa
authors.initials: E
authors.lastname: Locardi
authors.forename: Murray
authors.initials: M
authors.lastname: Goodman
mesh_terms: D002942:Circular Dichroism; D003094:Collagen / Q000737:chemistry*; D005029:Ethylenediamines / Q000737:chemistry*; D008958:Models, Molecular; D018716:Molecular Mimicry; D019906:Nuclear Magnetic Resonance, Biomolecular; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D013816:Thermodynamics
chemical_list: D005029:Ethylenediamines; D009842:Oligopeptides; C099539:tris(2-aminoethyl)amine; D003094:Collagen