Effect of oxidized LDL on hemolytic resistance of erythrocyte.
Using the method of peroxide-induced chemiluminescence we showed that incubation of the whole blood with oxidized LDL or oxidized blood plasma increased plasma hemoglobin concentration, which linearly depended on the degree of LDL oxidation. Similar effects were observed in erythrocyte suspension. Hemolytic activity of oxidized plasma 3-4-fold surpassed that of LDL isolated by ultracentrifugation. LDL capacity to oxidation in the presence of Cu(2+)increased by 50% and osmotic hemolysis of erythrocytes increased by 53% in coronary patients in comparison with healthy donors. These results indicate that oxidized LDL induce erythrocyte hemolysis.
Azizova
O A
OA
Institute of Physicochemical Medicine, Ministry of Health of the Russian Federation, Moscow.
Piryazev
A P
AP
Nikitina
N A
NA
Savchenkova
A P
AP
Lopukhin
Yu M
YM
D016022:Case-Control Studies; D004910:Erythrocyte Membrane / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008163:Luminescent Measurements
D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein