title: ACTH(1-24) down-regulates expression of ciliary neurotrophic factor mRNA in cultured rat astrocyte.
abstract: We examined the effects on astrocytes of ACTH, which is used to treat West syndrome. We stimulated cultured rat astrocytes with ACTH(1-24), corticotropin-releasing factor, and dexamethasone, and examined changes in neurotrophic factor mRNAs by reverse transcription-PCR. Down-regulation of ciliary neurotrophic factor mRNA expression was observed by stimulation with ACTH(1-24), but the expression of nerve growth factor, brain-derived neurotrophic factor, and nerotrophin-3 mRNAs was unaffected. Northern blot analysis revealed that the decrease in ciliary neurotrophic factor mRNA occurred 4 h after stimulation with more than 10 nM of ACTH(1-24). Up-regulation of nerotrophin-3 mRNA expression was found after stimulation with 1 mM dexamethasone. These results suggest that ACTH(1-24) administrated in West syndrome may influence the expression of neurotrophic factors in astrocytes in vivo.
authors.affiliation: Department of Pediatrics, Neonatology and Congenital Disorders, Nagoya City University Graduate School of Medical Sciences, Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Kokubo
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Asai
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Mineyoshi
authors.initials: M
authors.lastname: Aoyama
authors.forename: Masayuki
authors.initials: M
authors.lastname: Morikawa
authors.forename: Hajime
authors.initials: H
authors.lastname: Togari
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Wada
authors.forename: Taiji
authors.initials: T
authors.lastname: Kato
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism*; D001483:Base Sequence; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics; D002478:Cells, Cultured; D020934:Ciliary Neurotrophic Factor / Q000235:genetics*; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology; D003366:Cosyntropin / Q000494:pharmacology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D004247:DNA / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007223:Infant; D020932:Nerve Growth Factor / Q000235:genetics; D020933:Neurotrophin 3 / Q000235:genetics; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013036:Spasms, Infantile / Q000188:drug therapy / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D020934:Ciliary Neurotrophic Factor; D004791:Enzyme Inhibitors; D020933:Neurotrophin 3; D012333:RNA, Messenger; D003366:Cosyntropin; D003907:Dexamethasone; D004247:DNA; D003346:Corticotropin-Releasing Hormone; D020932:Nerve Growth Factor; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C