title: The seroprevalence of Helicobacter pylori and nitric oxide in acne rosacea.
abstract: BACKGROUND
  Acne rosacea is a dermatosis with unknown etiology. Some studies have reported a high prevalence of Helicobacter pylori infection in acne rosacea. Other studies have reported a decrease in the severity of the lesions of acne rosacea after eradication of H. pylori. H. pylori is a Gram-negative bacterium which colonizes the gastric mucosa and increases the synthesis of oxygen radicals, such as superoxide and proinflammatory cytokines. These cytokines have been demonstrated to stimulate the synthesis of the inflammatory species nitric oxide (NO). In this study, we examined the role of NO in the possible effect of H. pylori in acne rosacea.
  
  
  METHODS
  Thirty-three acne rosacea patients were included in the study and the control group comprised 20 healthy individuals. The levels of immunoglobulin G antibodies against H. pylori in the serum samples were measured using the enzyme-linked immunoabsorbent assay method. Measurement of nitrate was performed using chemiluminescence in accordance with the method described by Braman and Hendrix (Braman RS, Hendrix SA. Nanogram nitrite and nitrate determination in environmental and biological materials by vanadium(iii) reduction with chemiluminescence detection. Anal Chem 1989; 61: 2715-2718). For statistical analysis, the t-test was used.
  
  
  RESULTS
  The seropositivity of H. pylori in acne rosacea patients was found to be high; however, the serum nitrate levels were found to be normal.
  
  
  CONCLUSIONS
  The results of the study indicate that the inflammatory species NO, which has been hypothesized to be associated with H. pylori, has no role in the inflammatory mechanism of acne rosacea.
authors.affiliation: Department of Dermatology, Faculty of Medicine, Gazi University, Besevler, Ankara, Turkey.
authors.forename: Mehmet Ali
authors.initials: MA
authors.lastname: Gürer
authors.forename: Arzu
authors.initials: A
authors.lastname: Erel
authors.forename: Deniz
authors.initials: D
authors.lastname: Erbaş
authors.forename: Kayhan
authors.initials: K
authors.lastname: Cağlar
authors.forename: Ciğdem
authors.initials: C
authors.lastname: Atahan
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D016481:Helicobacter Infections / Q000097:blood / Q000453:epidemiology*; D016480:Helicobacter pylori / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood*; D012393:Rosacea / Q000453:epidemiology* / Q000276:immunology / Q000382:microbiology; D016036:Seroepidemiologic Studies
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D009569:Nitric Oxide