Evaluation of FIV protein-expressing VEE-replicon vaccine vectors in cats.
Venezuelan equine encephalitis (VEE) virus-replicon particles (VRP) were used to generate feline immunodeficiency virus (FIV) Gag- and ENV-expressing vaccine vectors. Serum and mucosal FIV-specific antibody was detected in cats immunized subcutaneously, once monthly for 5 months, with FIV-expressing VRP. Expansion of the CD8+ L-selectin negative phenotype and transient CD8+ noncytolytic suppressor activity were seen in cats immunized with FIV-expressing or control VRP. Despite induction of FIV-specific immune responses and nonspecific suppressor responses, all cats became infected following vaginal challenge with high dose, pathogenic cell-associated FIV-NCSU(1) although relative early maintenance of CD4+ cells was seen in FIV-immunized cats.
Burkhard
Mary Jo
MJ
Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, NC 27606, USA. maryjo_burkhard@ncsu.edu
Valenski
Loretta
L
Leavell
Sarah
S
Dean
Gregg A
GA
Tompkins
Wayne A F
WA
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D002415:Cats; D004666:Encephalitis Virus, Venezuelan Equine / Q000235:genetics; D005069:Evaluation Studies as Topic; D016181:Feline Acquired Immunodeficiency Syndrome / Q000276:immunology*; D005260:Female; D005822:Genetic Vectors / Q000235:genetics; D006023:Glycoproteins; D016089:Immunodeficiency Virus, Feline / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014621:Vagina / Q000276:immunology / Q000821:virology; D014759:Viral Envelope Proteins; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology; D014766:Viremia / Q000097:blood
D000914:Antibodies, Viral; D006023:Glycoproteins; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C445079:envelope protein, Feline immunodeficiency virus