title: PqqC/D, which converts a biosynthetic intermediate to pyrroloquinoline quinone.
abstract: PqqC/D was purified from Escherichia coli transformant. The purified enzyme converted an intermediate that accumulated in a pqqC mutant of Methylobacterium extorquens AM1 to PQQ. The reaction did not show any dependence of NAD(P)H that was observed in the crude extract before purification. PqqC/D reacted with the intermediate stoichiometrically, but not catalytically. When partially purified proteins from the crude extract of E. coli were added to the reaction mixture, the rate of PQQ production increased dependent on the amount of NADPH added and the total amount of PQQ produced increased.
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 753-8515, Yamaguchi, Japan. toyama@agr.yamaguchi-u.ac.jp
authors.forename: Hirohide
authors.initials: H
authors.lastname: Toyama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Fukumoto
authors.forename: Megumi
authors.initials: M
authors.lastname: Saeki
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Matsushita
authors.forename: Osao
authors.initials: O
authors.lastname: Adachi
authors.forename: Mary E
authors.initials: ME
authors.lastname: Lidstrom
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D020601:Methylobacterium extorquens / Q000201:enzymology*; D009249:NADP / Q000494:pharmacology; D045542:PQQ Cofactor; D017434:Protein Structure, Tertiary; D015363:Quinolones / Q000378:metabolism*; D011809:Quinones / Q000378:metabolism*; D017384:Sequence Deletion
chemical_list: D001426:Bacterial Proteins; C095467:PqqC protein, Bacteria; C091163:PqqD protein, Methylobacterium extorquens; D015363:Quinolones; D011809:Quinones; D009249:NADP; D045542:PQQ Cofactor