Construction of an Actinobacillus pleuropneumoniae serotype 2 prototype live negative-marker vaccine.
Deletions were introduced into the ureC and apxIIA genes of an Actinobacillus pleuropneumoniae serotype 2 strain by homologous recombination and counterselection. The double-mutant contains no foreign DNA, is highly attenuated, protects pigs from homologous challenge upon a single aerosol application, and facilitates the serological discrimination of immunized and infected herds.
Tonpitak
Walaiporn
W
Institut für Mikrobiologie und Tierseuchen, Tieraerztliche Hochschule Hannover, Germany.
Baltes
Nina
N
Hennig-Pauka
Isabel
I
Gerlach
Gerald F
GF
D000189:Actinobacillus Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000235:genetics; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology*; D017353:Gene Deletion; D006460:Hemolysin Proteins; D007114:Immunization; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology / Q000517:prevention & control*; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology*; D014774:Virulence
D000907:Antibodies, Bacterial; C085361:ApxII toxin, bacteria; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D006460:Hemolysin Proteins; D014613:Vaccines, Attenuated