Fluorometric detection of enzyme activity with synthetic supramolecular pores.
The reversible blockage of synthetic pores formed by rigid-rod beta barrels, either by substrates or products, was used to sense a variety of enzymatic reactions in high-throughput format with "naked-eye" fluorescent detection. Improvement of sensor sensitivity beyond three orders of magnitude by straightforward internal mutations underscores the functional plasticity of rigid-rod beta barrels. Such detectors of enzyme activity with the aforementioned characteristics are needed in areas as diverse as proteomics and environmentally benign organic synthesis.
Das
Gopal
G
Department of Organic Chemistry, University of Geneva, CH-1211 Geneva, Switzerland.
Talukdar
Pinaki
P
Matile
Stefan
S
D000255:Adenosine Triphosphate / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D001081:Apyrase / Q000378:metabolism*; D002384:Catalysis; D004798:Enzymes / Q000378:metabolism*; D005456:Fluorescent Dyes; D005470:Fluorometry; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005700:Galactosyltransferases / Q000378:metabolism; D008051:Lipid Bilayers; D010455:Peptides / Q000737:chemistry; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D020543:Proteome; D012680:Sensitivity and Specificity
D004798:Enzymes; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D010455:Peptides; D020543:Proteome; D000255:Adenosine Triphosphate; D005700:Galactosyltransferases; D000469:Alkaline Phosphatase; D001081:Apyrase; D005634:Fructose-Bisphosphate Aldolase