Monitoring the expression pattern of around 7,000 Arabidopsis genes under ABA treatments using a full-length cDNA microarray.
Full-length cDNAs are essential for functional analysis of plant genes. Recently, cDNA microarray analysis has been developed for quantitative analysis of global and simultaneous analysis of expression profiles. Microarray technology is a powerful tool for identifying genes induced by environmental stimuli or stress and for analyzing their expression profiles in response to environmental signals. We prepared an Arabidopsis full-length cDNA microarray containing around 7,000 independent full-length cDNA groups and analyzed the expression profiles of genes. The transcripts of 245, 299, 54 and 213 genes increased after abscisic acid (ABA), drought-, cold-, and salt-stress treatments, respectively, with inducibilities more than fivefold compared with those of control genes [corrected]. The cDNA microarray analysis showed that many ABA-inducible genes were induced after drought- and high-salinity-stress treatments, and that there is more crosstalk between drought and ABA responses than between ABA and cold responses. Among the ABA-inducible genes identified, we identified 22 transcription factor genes, suggesting that many transcriptional regulatory mechanisms exist in the ABA signal transduction pathways.
Seki
Motoaki
M
Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba 305-0074, Japan.
Ishida
Junko
J
Narusaka
Mari
M
Fujita
Miki
M
Nanjo
Tokihiko
T
Umezawa
Taishi
T
Kamiya
Asako
A
Nakajima
Maiko
M
Enju
Akiko
A
Sakurai
Tetsuya
T
Satou
Masakazu
M
Akiyama
Kenji
K
Yamaguchi-Shinozaki
Kazuko
K
Carninci
Piero
P
Kawai
Jun
J
Hayashizaki
Yoshihide
Y
Shinozaki
Kazuo
K
D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics*; D003080:Cold Temperature; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020411:Oligonucleotide Array Sequence Analysis; D009997:Osmotic Pressure; D011401:Promoter Regions, Genetic
D000040:Abscisic Acid