Vaginal and oral epithelial cell anti-Candida activity.
Candida albicans is the causative agent of acute and recurrent vulvovaginal candidiasis (VVC), a common mucosal infection affecting significant numbers of women in their reproductive years. While any murine host protective role for cell-mediated immunity (CMI), humoral immunity, and innate resistance by neutrophils against the vaginal infection appear negligible, significant in vitro growth inhibition of Candida species by vaginal and oral epithelial cell-enriched cells has been observed. Both oral and vaginal epithelial cell anti-Candida activity has a strict requirement for cell contact to C. albicans with no role for soluble factors, and oral epithelial cells inhibit C. albicans through a cell surface carbohydrate moiety. The present study further evaluated the inhibitory mechanisms by murine vaginal epithelial cells and the fate of C. albicans by oral and vaginal epithelial cells. Similar to human oral cells, anti-Candida activity produced by murine vaginal epithelial cells is unaffected by enzymatic cleavage of cell surface proteins and lipids but sensitive to periodic acid cleavage of surface carbohydrates. Analysis of specific membrane carbohydrate moieties, however, showed no role for sulfated polysaccharides, sialic acid residues, or glucose and mannose-containing carbohydrates, also similar to oral cells. Staining for live and dead Candida in the coculture with fluorescein diacetate (FDA) and propidium iodide (PI), respectively, showed a clear predominance of live organisms, suggesting a static rather than cidal action. Together, the results suggest that oral and vaginal epithelial cells retard or arrest the growth rather than kill C. albicans through an as-yet-unidentified carbohydrate moiety in a noninflammatory manner.
Nomanbhoy
Fatema
F
Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, New Orleans 70112, USA.
Steele
Chad
C
Yano
Junko
J
Fidel
Paul L
PL
D000818:Animals; D002176:Candida albicans / Q000254:growth & development / Q000276:immunology*; D002241:Carbohydrates / Q000276:immunology; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D018920:Coculture Techniques; D015169:Colony Count, Microbial; D004847:Epithelial Cells / Q000276:immunology; D005260:Female; D006801:Humans; D051379:Mice; D008808:Mice, Inbred CBA; D009061:Mouth Mucosa / Q000166:cytology / Q000276:immunology*; D014621:Vagina / Q000166:cytology / Q000276:immunology*
D002241:Carbohydrates