Characterization and chromosome assignment of the porcine AHCY gene for S-adenosylhomocysteine hydrolase.
The gene for S-adenosylhomocysteine hydrolase (AHCY) is involved in the regulation of cellular methylation reactions. Here we report the cloning, sequencing, and chromosomal assignment of the porcine AHCY gene. The gene consists of 10 exons spanning approximately 19 kb of genomic DNA. It encodes a protein of 432 amino acids that shows about 96% identity to the orthologous S-adenosylhomocysteine hydrolases from human, rat, and mouse. The porcine AHCY gene is located very close to the agouti signaling protein gene (ASIP) on SSC17q21. The chromosomal localization was subsequently confirmed by RH mapping and the genetic mapping of an intragenic microsatellite that maps to 57 cM on the linkage map of SSC17.
Leeb
T
T
Institute of Animal Breeding and Genetics, School of Veterinary Medicine Hannover, Hannover, Germany. Tosso.Leeb@tiho-hannover.de
Rohrer
G A
GA
D043383:Adenosylhomocysteinase; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005091:Exons; D006822:Hybrid Cells; D006867:Hydrolases / Q000235:genetics*; D007438:Introns; D020161:Physical Chromosome Mapping; D013552:Swine / Q000235:genetics* / Q000378:metabolism
D004247:DNA; D006867:Hydrolases; D043383:Adenosylhomocysteinase