Conserved homeodomain proteins interact with MADS box protein Mcm1 to restrict ECB-dependent transcription to the M/G1 phase of the cell cycle.
Two homeodomain proteins, Yox1 and Yhp1, act as repressors at early cell cycle boxes (ECBs) to restrict their activity to the M/G1 phase of the cell cycle in budding yeast. These proteins bind to Mcm1 and to a typical homeodomain binding site. The expression of Yox1 is periodic and directly correlated with its binding to, and repression of, ECB activity. The absence of Yox1 and Yhp1 or the constitutive expression of Yox1 leads to the loss of cell-cycle regulation of ECB activity. Therefore, the cell-cycle-regulated expression of these repressors defines the interval of ECB-dependent transcription. Twenty-eight genes, including MCM2-7, CDC6, SWI4, CLN3, and a number of genes required during late M phase have been identified that are coordinately regulated by this pathway.
Pramila
Tata
T
Fred Hutchinson Cancer Research Center, Basic Sciences Division, Seattle, Washington 98109-1024, USA.
Miles
Shawna
S
GuhaThakurta
Debraj
D
Jemiolo
Dave
D
Breeden
Linda L
LL
D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins; D003503:Cyclobutanes / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D018816:Genes, cdc / Q000502:physiology; D018398:Homeodomain Proteins; D006801:Humans; D007700:Kinetics; D026341:Minichromosome Maintenance 1 Protein / Q000502:physiology*; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins
D018797:Cell Cycle Proteins; D003503:Cyclobutanes; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D026341:Minichromosome Maintenance 1 Protein; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; C080459:Yox1 protein, S cerevisiae; C470232:didecyl squarate