Generation of a sequence characterized amplified region probe for authentication of crocodilian species.
A 209-base pair (bp) crocodilian-specific sequence characterized amplified region (SCAR) was identified from a 425-bp randomly amplified polymorphic DNA (RAPD) fragment. The 209-bp SCAR was produced from amplifications of DNA extracted from fresh and/or dry meat samples from at least three species of Crocodylus, Caiman crocodylus, and Alligator mississippiensis. No amplification was observed from DNA of other common animal species. The use of SCAR opens the way for quick authentication of crocodilian samples for conservation biology and trade regulation.
Yau
Forrest Chung-Fai
FC
Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
Wong
Ka-Lok
KL
Wang
Jun
J
But
Paul Pui-Hay
PP
Shaw
Pang-Chui
PC
D000489:Alligators and Crocodiles / Q000145:classification* / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003132:Commerce; D015342:DNA Probes / Q000235:genetics*; D004463:Ecology; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity
D015342:DNA Probes; D012336:RNA, Ribosomal, 16S