Quality assessment of Mycobacterium tuberculosis genotyping in a large laboratory network.
Quality assessment exercises were conducted to evaluate the reproducibility of IS6110 DNA fingerprinting performed by eight laboratories in the National Tuberculosis Genotyping and Surveillance Network. Three panels, each with 8 to 16 isolates, were typed at all laboratories, resulting in 280 images. When the pattern obtained by the majority for each isolate was used as the standard, exact matches were obtained for 73% of patterns; 90% and 97% of patterns matched within one- and two-band differences, respectively. A second approach involved retyping of randomly selected isolates at the Centers for Disease Control and Prevention. Retyping was done for 8-19 isolates per laboratory (76 total). Paired images matched exactly for 54% of isolates and within one and two band differences, 78% and 93%, respectively. We evaluated reasons for mismatching. We also evaluated the reproducibility of spoligotyping using a test panel of 13 isolates; a discrepancy of 1 in 91 results was noted.
Braden
Christopher R
CR
Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. cbraeden@cdc.gov
Crawford
Jack T
JT
Schable
Barbara A
BA
D015373:Bacterial Typing Techniques / Q000592:standards*; D002487:Centers for Disease Control and Prevention, U.S. / Q000592:standards; D016172:DNA Fingerprinting / Q000592:standards; D004269:DNA, Bacterial / Q000032:analysis; D005838:Genotype; D006801:Humans; D007753:Laboratories / Q000458:organization & administration / Q000592:standards*; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D014376:Tuberculosis / Q000175:diagnosis / Q000382:microbiology / Q000517:prevention & control; D014481:United States
D004269:DNA, Bacterial