title: A new method for fish leucocyte counting and partial differentiation by flow cytometry.
abstract: A simple and rapid method for analysis of fish blood cells is presented. Carp (Cyprinus carpio) blood was diluted 200 times with Hanks' solution containing 1 microg/ml of DiOC6(3) which is a fluorescent, lipophilic dye. After staining for 10 min, the blood cells were measured by a flow cytometer (FACS). Several blood cell populations were identified by different FL-1 (green fluorescence), FSC (forward scatter), and SSC (side scatter) properties. FL-1 v. SSC or FSC v. SSC dot-plot of stained blood cells displayed five separate cell populations: erythrocytes: a mixture of thrombocytes plus lymphocytes; monocytes; neutrophils; and basophils. The number of each type of blood cell counted by the FACS was in good agreement with those counted microscopically.
authors.affiliation: Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Kameino 1866, Fujisawa, Kanagawa, 252-8510 Japan.
authors.forename: Takamasa
authors.initials: T
authors.lastname: Inoue
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Moritomo
authors.forename: Yumi
authors.initials: Y
authors.lastname: Tamura
authors.forename: Sachiko
authors.initials: S
authors.lastname: Mamiya
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fujino
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nakanishi
mesh_terms: D000818:Animals; D001772:Blood Cell Count / Q000379:methods / Q000662:veterinary*; D002347:Carps / Q000097:blood*; D002469:Cell Separation / Q000379:methods / Q000662:veterinary*; D004906:Erythrocyte Count / Q000379:methods / Q000662:veterinary; D005434:Flow Cytometry / Q000379:methods / Q000662:veterinary*; D005456:Fluorescent Dyes; D007958:Leukocyte Count / Q000379:methods / Q000662:veterinary; D007962:Leukocytes; D018655:Lymphocyte Count / Q000379:methods / Q000662:veterinary; D010976:Platelet Count / Q000379:methods / Q000662:veterinary; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes