title: Glycosaminoglycan-binding ability is a feature of wild-type strains of herpes simplex virus type 1.
abstract: Adaptation of some viruses to replication in cultured cells selects variants that due to alterations in the viral attachment proteins convert to using heparan sulfate (HS) as initial receptor. We report that the nucleotide sequence of herpes simplex virus type 1 (HSV-1) glycoprotein C (gC), a principal attachment component of the virus, remained unchanged during adaptation of wild-type strains to cultured cells. Likewise, amino acid residues critical for binding of gC to HS were conserved in viral strains that replicated in vivo in different human tissues. Moreover wild-type HSV-1 strains derived directly from clinical specimens were, similar to their cell culture propagated progeny viruses and common laboratory strains, sensitive to heparin and demonstrated impairment in their ability to infect HS/chondroitin sulfate deficient cells. These results demonstrate that the HS-binding ability is a feature of wild-type strains of HSV-1.
authors.affiliation: Department of Clinical Virology, Göteborg University, Guldhedsgatan 10B, S-413 46 Göteborg, Sweden.
authors.forename: Edward
authors.initials: E
authors.lastname: Trybala
authors.forename: Anette
authors.initials: A
authors.lastname: Roth
authors.forename: Maria
authors.initials: M
authors.lastname: Johansson
authors.forename: Jan-Ake
authors.initials: JA
authors.lastname: Liljeqvist
authors.forename: Elham
authors.initials: E
authors.lastname: Rekabdar
authors.forename: Olle
authors.initials: O
authors.lastname: Larm
authors.forename: Tomas
authors.initials: T
authors.lastname: Bergström
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D002522:Chlorocebus aethiops; D006025:Glycosaminoglycans / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism; D006561:Herpes Simplex / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D006801:Humans; D007739:L Cells; D051379:Mice; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1