Optimization and validation of an analytical procedure by high-performance liquid chromatography for the quantification of peroxisomicines and isoperoxisomicines.
Peroxisomicine A1 is a potential antineoplastic substance extracted from plants of the genus Karwinskia. An RP-HPLC-DAD method was developed and validated for the separation and quantification of four isomers of this compound. These isomers coelute in the preparative procedure and are present at a proportion ranging between 3 and 5% in the peroxisomicine A1 purified in the laboratory. The desirability coefficient of the method described here was enhanced 140% with respect to the previously reported method.
Osorio-Pérez
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A
Department of Pharmacology and Toxicology, Faculty of Medicine, U.A.N.L., P.O. Box 2316, Suc. Tecnológico, 64841, Nuevo Leon, Monterrey, Mexico
Salazar-Cavazos
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M
Piñeyro-López
A
A
Waksman de Torres
N
N
D000873:Anthracenes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results
D000873:Anthracenes