title: Purification and characterization of a rhamnose-binding lectin with immunoenhancing activity from grass carp (Ctenopharyngodon idellus) ovaries.
abstract: A rhamnose-specific lectin was isolated from ovaries of the grass carp (Ctenopharyngodon idellus). The grass carp lectin possesses a molecular mass of 205 kDa. It is composed of six subunits each with a molecular mass of 35 kDa. The N-terminal amino acid sequence of the grass carp shows similarity to those of other fish species with 26-35% amino acid identity. It is mitogenic toward murine splenocytes and peritoneal exudate cells.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Basic Medical Sciences Bldg. Rm. 302B, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
authors.forename: Y W
authors.initials: YW
authors.lastname: Lam
authors.forename: T B
authors.initials: TB
authors.lastname: Ng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006384:Hemagglutination / Q000187:drug effects; D007107:Immune System / Q000187:drug effects* / Q000378:metabolism; D007371:Interferon-gamma / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010053:Ovary / Q000737:chemistry*; D012210:Rhamnose / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D017385:Sequence Homology; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: D007376:Interleukin-2; D037102:Lectins; D008934:Mitogens; D007371:Interferon-gamma; D012210:Rhamnose