title: Cysteine-rich secretory protein-3: a potential biomarker for prostate cancer.
abstract: Electronic profiling of publicly available expressed sequence tag databases identified a gene, cysteine-rich secretoryprotein-3 (CRISP-3), that is up-regulated in prostate cancer, and of which the expression is relatively prostate-specific. The objective of this study was to examine the potential of CRISP-3 as a biomarker for prostate cancer. In transient transfection studies, CRISP-3 was found to be a secretory protein. Using a multiple tissue dot blot experiment, CRISP-3 transcript was identified in a limited number of human tissues including the prostate. In situ hybridization experiments indicated that CRISP-3 mRNA is epithelial-specific and is up-regulated in prostate adenocarcinoma compared with benign prostate tissue. CRISP-3 mRNA overexpression in cancer was confirmed using quantitative real-time reverse-transcription-PCR using benign prostatic epithelia and adenocarcinoma (in 5 of 5 cases) isolated by laser capture microdissection, as well as bulk tissues (in 20 of 23 cases) from surgically resected human prostates. These findings suggest that CRISP-3 is a potential biomarker for prostate cancer.
authors.affiliation: Division of Experimental Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.
authors.forename: Farhad
authors.initials: F
authors.lastname: Kosari
authors.forename: Yan W
authors.initials: YW
authors.lastname: Asmann
authors.forename: John C
authors.initials: JC
authors.lastname: Cheville
authors.forename: George
authors.initials: G
authors.lastname: Vasmatzis
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000235:genetics; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D019295:Computational Biology; D004581:Electronics; D004847:Epithelial Cells / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D015723:Gene Library; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D009369:Neoplasms / Q000097:blood / Q000235:genetics; D017430:Prostate-Specific Antigen / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D011471:Prostatic Neoplasms / Q000097:blood / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012471:Salivary Proteins and Peptides / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics*; D029589:Seminal Plasma Proteins / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics*; D012680:Sensitivity and Specificity; D014162:Transfection; D015854:Up-Regulation / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C489926:CRISP3 protein, human; D012333:RNA, Messenger; D012471:Salivary Proteins and Peptides; D029589:Seminal Plasma Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D017430:Prostate-Specific Antigen