Molecular cloning and phylogenetic analysis of Babesia gibsoni heat shock protein 70.
The Babesia gibsoni heat shock protein 70 gene (BGHsp70) was cloned by polymerase chain reaction (PCR) and sequenced. The length of the gene was 1938 bp and the predicted polypeptide was 646 amino acids long with a calculated molecular weight of 70,627. The amino acid sequences of BGHsp70 from 17 isolates were identical, though there were six types of polymorphisms among the corresponding nucleotide sequences. There was no intron in the BGHsp70 gene. Phylogenetic analysis of the amino acid sequence of Hsp70 showed that B. gibsoni was most closely related to B. bovis and lies within a phylogenetic cluster with Theileria. These results suggest that Hsp70 was well conserved among intraerythrocytic protozoa.
Yamasaki
M
M
Laboratory of Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Tajima
M
M
Lee
K W
KW
Jeong
J R
JR
Yamato
O
O
Maede
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001403:Babesia / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004285:Dogs; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D018840:HSP70 Heat-Shock Proteins; D012313:RNA