Inorganic-organic polymer hybrid scaffold for tissue engineering--II: partial enzymatic degradation of hydroxyapatite-chitosan hybrid.
This article describes the preparation of porous chitosan, a hydroxyapatite hybrid, by partial enzymatic degradation. Two enzymes, chitosanase and lysozyme, were selected to hydrolyze a chitosan-reinforced matrix and create pores within the chitosan-hydroxyapatite composite. The degree of enzymatic hydrolysis of the chitosan-hydroxyapatite composite was determined by measuring the % weight loss of the chitosan matrix and the hydroxyapatite component. Hydroxyapatite loss from the chitosan matrix increased with the degree of enzymatic hydrolysis of the chitosan-reinforced matrix. After hydrolysis, the composite was further characterized by FTIR. Quantitative analysis revealed a decrease in the characteristic pyranose ring peak (1072 cm(-1)), compared with Po4(2-) (1110 cm(-1)), showing that the chitosan matrices were enzymatically hydrolyzed. The surface of the porous chitosan-hydroxyapatite composite, prepared by controlling enzymatic hydrolysis, was also observed by SEM.
Tachaboonyakiat
Wanpen
W
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Japan.
Serizawa
Takeshi
T
Akashi
Mitsuru
M
D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism; D048271:Chitosan; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000378:metabolism; D020100:Hydrogels / Q000378:metabolism; D006882:Hydroxyapatites / Q000737:chemistry* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*
D020100:Hydrogels; D006882:Hydroxyapatites; D011108:Polymers; D002686:Chitin; D048271:Chitosan; D006026:Glycoside Hydrolases; C019868:chitosanase; D009113:Muramidase