Importance of TP53 and RB in the repair of potentially lethal damage and induction of color junctions after exposure to ionizing radiation.
Repair of potentially lethal damage (PLD) was investigated in cells with functional G1-phase arrest with wild-type TP53 and wild-type RB and in cells in which G1-phase arrest was abrogated by inactivation of TP53 or RB. Confluent cultures of cells were plated for clonogenic survival assay either immediately or 24 h after irradiation. Induction of color junctions, an exchange between a painted and unpainted chromosome, was studied in chromosomes 18 and 19 after irradiation with 4 Gy gamma rays. Significant repair of PLD was found in cells carrying both wild-type TP53 and wild-type RB. In cells in which TP53 or RB was inactivated, the survival curves from immediately plated and delayed-plated cells were not significantly different. The numbers of radiation-induced color junctions in chromosomes 18 and 19 were similar in all cell lines. From this study we conclude that a functional G1-phase arrest is important for repair of PLD and that TP53 and RB do not affect the frequencies of induction of color junctions in chromosome 18 or 19.
Franken
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NA
Department of Radiotherapy, University of Amsterdam, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands. N.A.Franken@amc.nl
van Bree
C
C
ten Cate
R
R
van Oven
C H
CH
Haveman
J
J
D015153:Blotting, Western; D002869:Chromosome Aberrations; D002887:Chromosomes, Human, Pair 18 / Q000528:radiation effects; D002888:Chromosomes, Human, Pair 19 / Q000528:radiation effects; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D016193:G1 Phase / Q000528:radiation effects; D005720:Gamma Rays; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008677:Metaphase; D011839:Radiation, Ionizing; D016160:Retinoblastoma Protein / Q000235:genetics*; D014407:Tumor Cells, Cultured
D016160:Retinoblastoma Protein; D004247:DNA