title: Pathological characteristics, PCNA labeling index and DNA index in prognostic evaluation of patients with moderately differentiated hepatocellular carcinoma.
abstract: OBJECTIVE
  To study the relationship between prognosis and pathological characteristics, proliferating cell nuclear antigen labeling index (PCNA-LI) and DNA index (DI) in patients with moderately differentiated hepatocellular carcinoma(HCC).
  
  
  METHODS
  51 cases of moderately differentiated HCC were analyzed with respect to the relation between their clinical follow-up data and pathological characteristics. Meanwhile, PCNA-LI of HCC cells was detected by immunohistochemistry assay and DI was measured by Feulgen staining and automatic image analysis technique.
  
  
  RESULTS
  Patients with a single tumor nodule, less than 5 cm in diameter, no tumor emboli, no daughter nodules and necrosis had relatively better prognosis; patients with euploidy HCC had better prognosis than those with aneuploidy; among the aneuploidy patients those with DI<1.5 had better prognosis than the cases with DI>1.5; The higher the PCNA-LI, the worse would be the prognosis. The increase in DI was correlated with the increase in PCNA-LI, and both of them were correlated with the pathological changes of the tumor.
  
  
  CONCLUSIONS
  A composite analysis of the pathological characteristics of tumor tissue, DI and PCNA-LI might be useful in predicting the prognosis of HCC patients.
authors.affiliation: Department of Pathology, Medical School of Fudan University, Shanghai, China.
authors.forename: Wen-Jiao
authors.initials: WJ
authors.lastname: Zeng
authors.forename: Guo-Yuan
authors.initials: GY
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Xin-Da
authors.initials: XD
authors.lastname: Zhou
authors.forename: Yue-E
authors.initials: YE
authors.lastname: Zhang
authors.forename: Nong
authors.initials: N
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000782:Aneuploidy; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D002454:Cell Differentiation; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*
chemical_list: D004273:DNA, Neoplasm; D018809:Proliferating Cell Nuclear Antigen