Contribution of protein synthesis to mechanisms of antiepileptic system activity in kindling.
We studied changes in protein composition and their effects on convulsive activity in drug-induced and electrical kindling. Injection of protein fraction of brain extract from animals subjected to metronidazole-induced kindling to intact recipients reduced their sensitivity to the convulsant (i. e. increased the dose of metronidazole inducing clonic convulsions and lethal seizures). Incorporation of labeled amino acids in proteins in different brain structures after appearance of stable afterdischarges in the zone of synaptic stimulation significantly decrease compared to the control. Label incorporation decreased also in the frontal and occipital cortex. Kindling-associated changes in the protein spectrum were studied by electrophoresis.
Karpova
M N
MN
Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow.
Karganov
M Yu
MY
Klishina
N Yu
NY
Khlebnikova
N N
NN
Kryzhanovskii
G N
GN
D000818:Animals; D001921:Brain / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004827:Epilepsy / Q000378:metabolism / Q000517:prevention & control*; D007696:Kindling, Neurologic / Q000378:metabolism*; D008795:Metronidazole; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains; D009419:Nerve Tissue Proteins / Q000502:physiology*; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D008795:Metronidazole