Immunohistochemical detection of polychlorinated biphenyls in field collected damselfish (Abudefduf sordidus; Pomacentridae) embryos and larvae.
Antibodies against polychlorinated biphenyls (PCBs) were used to determine if immunohistochemical methods could detect PCBs in embryos and larvae of a territorial coral reef fish (Abudefduf sordidus; Pomacentridae) collected from Johnston Atoll, Central Pacific Ocean. Sites with differing levels of contamination were sampled, one with relatively high sediment PCB concentrations of up to 389.0 ng/g and another with low PCB concentrations of only 0.5 ng/g. Immunostaining suggested that PCB concentrations were higher in fish larvae from the PCB contaminated site and that PCB concentrations within abnormal embryos were higher than normal embryos from the same nest. This technique will be useful for detecting exposed populations in the field and assessing correlations with adverse effects, particularly in potential indicator organisms such as Abudefduf sordidus.
Lobel
Lisa M Kerr
LM
Department of Biology, University of Massachusetts, Boston 02125, USA. lisa.lobel@umb.edu
Davis
Elizabeth A
EA
D000818:Animals; D037421:Anthozoa; D004625:Embryo, Nonmammalian / Q000737:chemistry; D004784:Environmental Monitoring / Q000379:methods*; D005399:Fishes / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D007814:Larva / Q000737:chemistry; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D012623:Seawater; D014874:Water Pollutants, Chemical / Q000032:analysis*
D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls