title: Distribution of extended-spectrum beta-lactamases in clinical isolates of Enterobacteriaceae in Vietnam.
abstract: Among 730 Escherichia coli, 438 Klebsiella pneumoniae, and 141 Proteus mirabilis isolates obtained between September 2000 and September 2001 in seven hospitals in Ho Chi Minh City, Vietnam, 26.6% were resistant to ceftazidime, 30% were resistant to cefotaxime, 31.5% were resistant to ceftriaxone, 15.9% were resistant to cefoperazone, and 6% were resistant to cefepime. Resistance to imipenem was found in 5.6% of the isolates. In 55 strains producing extended-spectrum beta-lactamases (32 E. coli isolates, 13 K. pneumoniae isolates, and 10 P. mirabilis isolates), structural genes for VEB-1 (25.5%), CTX-M (25.5%), SHV (38.1%), and TEM (76.3%) enzymes were detected alone or in combination. Sequencing of the PCR products obtained from the K. pneumoniae isolates revealed the presence of bla(VEB-1), bla(CTX-M-14), bla(CTX-M-17), bla(SHV-2), and bla(TEM-1). Molecular typing of the strains with a similar resistance phenotype to broad-spectrum cephalosporins indicated polyclonal spread. ISEcp1 was presumably responsible for dissemination of the bla(CTX-M-like) gene.
authors.affiliation: Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15, France.
authors.forename: Van
authors.initials: V
authors.lastname: Cao
authors.forename: Thierry
authors.initials: T
authors.lastname: Lambert
authors.forename: Duong Quynh
authors.initials: DQ
authors.lastname: Nhu
authors.forename: Huynh Kim
authors.initials: HK
authors.lastname: Loan
authors.forename: Nguyen Kim
authors.initials: NK
authors.lastname: Hoang
authors.forename: Guillaume
authors.initials: G
authors.lastname: Arlet
authors.forename: Patrice
authors.initials: P
authors.lastname: Courvalin
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004755:Enterobacteriaceae / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010641:Phenotype; D015995:Prevalence; D014744:Vietnam; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases