title: The diagnosis of brucellosis by use of BACTEC 9240 blood culture system.
abstract: The diagnosis of brucellosis is generally made when a standard tube agglutination titer of 1/160 or more for anti-Brucella antibodies in the presence of compatible clinical signs and symptoms. However isolation of the organism from blood or bone marrow is the proof of the disease. In this study we aimed to describe the rate and duration of isolation of Brucella spp. from blood and bone marrow by use of automated blood culture system (BACTEC 9240). Between 1997 to 2001, 23 adults were diagnosed as brucellosis. Blood culture was obtained in all and simultaneous bone marrow culture in 18 and both specimens were cultured by BACTEC 9240. Brucella was isolated from blood and bone marrow cultures in 19 (82.6%) and 13 (81.2%) respectively. All positive blood cultures yielded within 7 days and bone marrow cultures in 4 days. We concluded that automated BACTEC culture systems can isolate Brucella spp. in a fast and efficient way.
authors.affiliation: Department of Clinical Bacteriology and Infectious Diseases, University of Istanbul 34303, Aksaray, Istanbul, Turkey.
authors.forename: Recep
authors.initials: R
authors.lastname: Ozturk
authors.forename: Ali
authors.initials: A
authors.lastname: Mert
authors.forename: Funda
authors.initials: F
authors.lastname: Kocak
authors.forename: Resat
authors.initials: R
authors.lastname: Ozaras
authors.forename: Fatma
authors.initials: F
authors.lastname: Koksal
authors.forename: Fehmi
authors.initials: F
authors.lastname: Tabak
authors.forename: Muammer
authors.initials: M
authors.lastname: Bilir
authors.forename: Yildirim
authors.initials: Y
authors.lastname: Aktuglu
mesh_terms: D000328:Adult; D001331:Automation; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology; D001853:Bone Marrow / Q000382:microbiology; D002002:Brucella / Q000302:isolation & purification*; D002006:Brucellosis / Q000097:blood / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008297:Male; D011446:Prospective Studies; D012680:Sensitivity and Specificity