title: Quantitative determination of bile salt hydrolase activity in bacteria isolated from the small intestine of chickens.
abstract: A quantitative assay based on high-performance liquid chromatography analysis of bile salts and bacterial protein determination was established for investigating bile salt hydrolase (BSH) activity in bacteria isolated from the small intestine of chickens. Bacteria were isolated using various media and were subsequently grouped according to cell morphology, fermentation profile, and 16S ribosomal DNA sequence. Representative isolates from each bacterial group were assayed for BSH activity. The isolates differed in BSH activity with respect to the state of growth and preculturing with and without taurochenodeoxycholate. The highest levels of BSH activity were found with Enterococcus faecium and Clostridium perfringens.
authors.affiliation: Danish Institute of Agricultural Sciences, Department of Animal Nutrition and Physiology, Research Centre Foulum, DK-8830 Tjele, Denmark. ane.knarreborg@agrsci.dk
authors.forename: Ane
authors.initials: A
authors.lastname: Knarreborg
authors.forename: Ricarda M
authors.initials: RM
authors.lastname: Engberg
authors.forename: Søren K
authors.initials: SK
authors.lastname: Jensen
authors.forename: Bent B
authors.initials: BB
authors.lastname: Jensen
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism*; D000818:Animals; D001419:Bacteria / Q000201:enzymology*; D001483:Base Sequence; D002645:Chickens; D003016:Clostridium perfringens / Q000201:enzymology; D013293:Enterococcus faecalis / Q000201:enzymology; D007421:Intestine, Small / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data
chemical_list: D000581:Amidohydrolases; C023154:choloylglycine hydrolase