title: Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype Reston by the use of recombinant nucleoprotein-expressing HeLa cells.
abstract: An indirect immunofluorescent assay (IFA) to detect Ebola virus subtype Reston (EBO-R) antibodies was developed by the use of a HeLa cell line stably expressing EBO-R nucleoprotein (NP). This IFA has a high specificity for the detection of EBO-R IgG antibodies in both hyperimmune rabbit sera and monkey sera collected during an EBO-R outbreak in the Philippines in 1996. Furthermore, this IFA showed a higher sensitivity for the detection of EBO-R antibodies than did the IFA using HeLa cells expressing the NP of Ebola virus subtype Zaire. These results suggest that this new IFA is useful for seroepidemiological studies of EBO-R infection among monkeys.
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Ikegami
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saijo
authors.forename: Masahiro
authors.initials: M
authors.lastname: Niikura
authors.forename: Mary E G
authors.initials: ME
authors.lastname: Miranda
authors.forename: Alan B
authors.initials: AB
authors.lastname: Calaor
authors.forename: Marvin
authors.initials: M
authors.lastname: Hernandez
authors.forename: Daria L
authors.initials: DL
authors.lastname: Manalo
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshikawa
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morikawa
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D029043:Ebolavirus / Q000276:immunology* / Q000302:isolation & purification; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006367:HeLa Cells; D019142:Hemorrhagic Fever, Ebola / Q000276:immunology; D006801:Humans; D008252:Macaca fascicularis; D008992:Monkey Diseases / Q000821:virology; D009698:Nucleoproteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D011817:Rabbits; D011994:Recombinant Proteins / Q000378:metabolism; D012680:Sensitivity and Specificity; D014162:Transfection / Q000379:methods
chemical_list: D000914:Antibodies, Viral; D009698:Nucleoproteins; D011994:Recombinant Proteins