title: MHC class II compartments in human dendritic cells undergo profound structural changes upon activation.
abstract: Immature dendritic cells efficiently capture exogenous antigens in peripheral tissues. In an inflammatory environment, dendritic cells are activated and become highly competent antigen-presenting cells. Upon activation, they lose their ability for efficient endocytosis and gain capability to migrate to secondary lymphoid organs. In addition, peptide loading of MHC class II molecules is enhanced and MHC class II/peptide complexes are redistributed from an intracellular location to the plasma membrane. Using immuno-electron microscopy, we show that activation of human monocyte-derived dendritic cells induced striking modifications of the lysosomal multilaminar MHC class II compartments (MIICs), whereby electron-dense tubules and vesicles emerged from these compartments. Importantly, we observed that MHC class II expression in these tubules/vesicles transiently increased, while multilaminar MIICs showed a strongly reduced labeling of MHC class II molecules. This suggests that formation of the tubules/vesicles from multilaminar MIICs could be linked to transport of MHC class II from these compartments to the cell surface. Further characterization of endocytic organelles with lysosomal marker proteins, such as the novel dendritic cell-specific lysosomal protein DC-LAMP, HLA-DM and CD68, revealed differential sorting of these markers to the tubules and vesicles.
authors.affiliation: Department of Cell Biology and Institute of Biomembranes, Utrecht University School of Medicine, 3584 CX Utrecht, the Netherlands.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Barois
authors.forename: Blandine
authors.initials: B
authors.lastname: de Saint-Vis
authors.forename: Serge
authors.initials: S
authors.lastname: Lebecque
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Geuze
authors.forename: Monique J
authors.initials: MJ
authors.lastname: Kleijmeer
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism*; D004583:Electrons; D004705:Endocytosis; D005434:Flow Cytometry; D005802:Genes, MHC Class II; D006681:HLA-D Antigens / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D051907:Lysosomal Membrane Proteins; D008247:Lysosomes / Q000378:metabolism; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D009000:Monocytes / Q000378:metabolism; D013997:Time Factors; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D006681:HLA-D Antigens; D051907:Lysosomal Membrane Proteins; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C084245:HLA-DM antigens