New technique (the NOW test) for rapid detection of Streptococcus pneumoniae in the nasopharynx.
Although the NOW test was originally introduced as a rapid pneumococcal antigen detection test for use with urine samples, it was successfully adapted to nasopharyngeal samples in the present study. The sensitivity, specificity, positive predictive value, and negative predictive value of the test were 92.2, 97.7, 95.9, and 95.5%, respectively. These results demonstrate that nasopharyngeal colonization with Streptococcus pneumoniae can be documented within 15 min of sample collection.
Faden
Howard
H
Department of Pediatrics, State University of New York, University of Buffalo, Buffalo, New York, USA. hfaden@upa.chob.edu
Heimerl
Michael
M
Goodman
Gail
G
Winkelstein
Peter
P
Varma
Chelikani
C
D000293:Adolescent; D000942:Antigens, Bacterial / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D002845:Chromatography; D005260:Female; D006801:Humans; D007118:Immunoassay; D007223:Infant; D008297:Male; D009305:Nasopharynx / Q000382:microbiology*; D010033:Otitis Media / Q000382:microbiology; D011008:Pneumococcal Infections / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013296:Streptococcus pneumoniae / Q000302:isolation & purification*; D013997:Time Factors
D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial; D011933:Reagent Kits, Diagnostic