title: Maintenance of mitochondrial morphology is linked to maintenance of the mitochondrial genome in Saccharomyces cerevisiae.
abstract: In the yeast Saccharomyces cerevisiae, certain mutant alleles of YME4, YME6, and MDM10 cause an increased rate of mitochondrial DNA migration to the nucleus, carbon-source-dependent alterations in mitochondrial morphology, and increased rates of mitochondrial DNA loss. While single mutants grow on media requiring mitochondrial respiration, any pairwise combination of these mutations causes a respiratory-deficient phenotype. This double-mutant phenotype allowed cloning of YME6, which is identical to MMM1 and encodes an outer mitochondrial membrane protein essential for maintaining normal mitochondrial morphology. Yeast strains bearing null mutations of MMM1 have altered mitochondrial morphology and a slow growth rate on all carbon sources and quantitatively lack mitochondrial DNA. Extragenic suppressors of MMM1 deletion mutants partially restore mitochondrial morphology to the wild-type state and have a corresponding increase in growth rate and mitochondrial DNA stability. A dominant suppressor also suppresses the phenotypes caused by a point mutation in MMM1, as well as by specific mutations in YME4 and MDM10.
authors.affiliation: Department of Molecular Biology, University of Wyoming, Laramie 82071-3944, USA.
authors.forename: Theodor
authors.initials: T
authors.lastname: Hanekamp
authors.forename: Mary K
authors.initials: MK
authors.lastname: Thorsness
authors.forename: Indrani
authors.initials: I
authors.lastname: Rebbapragada
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Fisher
authors.forename: Corrine
authors.initials: C
authors.lastname: Seebart
authors.forename: Monica R
authors.initials: MR
authors.lastname: Darland
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Coxbill
authors.forename: Dustin L
authors.initials: DL
authors.lastname: Updike
authors.forename: Peter E
authors.initials: PE
authors.lastname: Thorsness
mesh_terms: D004272:DNA, Mitochondrial; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D004272:DNA, Mitochondrial; C089419:MDM10 protein, S cerevisiae; C089418:MMM1 protein, S cerevisiae; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins