The protein encoded at the 3' end of the serine protease gene of Aeromonas sobria functions as a chaperone in the production of the protease.
For the successful production of Aeromonas sobria serine protease (ASP), open reading frame 2 (ORF2) protein, encoded at the 3' end of the protease operon, is required. In this study, we examined the action of ORF2 protein. The results showed that the protein associated with ASP in the periplasm and helped ASP to form an active structure.
Nomura
Tomohiko
T
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro, Japan. nomu@ph.bunri-u.ac.jp
Fujii
Yoshio
Y
Yamanaka
Hiroyasu
H
Kobayashi
Hidetomo
H
Okamoto
Keinosuke
K
D000333:Aeromonas / Q000201:enzymology*; D001426:Bacterial Proteins / Q000502:physiology*; D018832:Molecular Chaperones / Q000502:physiology*; D009876:Operon; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000235:genetics*
D001426:Bacterial Proteins; D018832:Molecular Chaperones; D012697:Serine Endopeptidases