An enhancer complex confers both high-level and cell-specific expression of the human type X collagen gene.
Type X collagen expression is restricted to hypertrophic chondrocytes in the endochondral growth plate. Transient transfection of reporter constructs containing the human collagen X promoter into primary growth plate chondrocytes identified a cis-acting positive regulatory DNA element(s) that has cell-specific enhancer properties and binds a nuclear protein expressed specifically in growth plate chondrocytes. Functional disruption of this region results in a significant reduction in the activation of reporter gene transcription. The identified enhancer is a major element controlling both high-level and cell-specific expression of type X collagen gene.
Chambers
David
D
Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences, 2.205 Stopford Building, Oxford Road, University of Manchester, M13 9PT, Manchester, UK.
Young
David A
DA
Howard
Charles
C
Thomas
J Terrig
JT
Boam
David S
DS
Grant
Michael E
ME
Wallis
Gillian A
GA
Boot-Handford
Raymond P
RP
D001483:Base Sequence; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D019902:Chondrocytes / Q000378:metabolism; D024121:Collagen Type X / Q000235:genetics*; D004247:DNA; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D011401:Promoter Regions, Genetic
D024121:Collagen Type X; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase