title: Expression of pejerrey gonadotropin-releasing hormone in three orders of fish.
abstract: Molecular variants of GnRH were characterized by reverse-phase, high-performance liquid chromatography from brain extracts of fish in three different orders: Synbranchiformes (swamp eel [Synbranchus marmoratus]), Cyprinidontiformes (platyfish [Xiphophorus maculatus] and green swordtail [X. helleri]), and Atheriniformes (Patagonia pejerrey [Odontesthes hatchery]). Also, pituitary gland extracts from the pejerrey O. bonariensis (Atheriniformes) were characterized. Eluted fractions were tested in radioimmunoassays with antisera specific to GnRH, including both antisera that detected only one form of GnRH and those that detected several forms. The results show that brain extracts obtained from all species contained the same three molecular forms of GnRH, which were immunologically and chromatographically undistinguishable from chicken GnRH-II, pejerrey GnRH (pjGnRH), and salmon GnRH. This study supports the hypothesis that expression of these three forms is common in different fish orders and that pjGnRH is the main regulator of pituitary function in these fish.
authors.affiliation: Instituto Tecnológico de Chascomús (IIB-INTECH, CONICET-Universidad de San Martín), Camino de Circunvalación Laguna, Km 6. CC 164 (B7130IWA) Chascomús, Provincia de Buenos Aires, Argentina. somoza@intech.gov.ar
authors.forename: G M
authors.initials: GM
authors.lastname: Somoza
authors.forename: D W
authors.initials: DW
authors.lastname: Lescheid
authors.forename: L A
authors.initials: LA
authors.lastname: Miranda
authors.forename: F L
authors.initials: FL
authors.lastname: Lo Nostro
authors.forename: L
authors.initials: L
authors.lastname: Magliulo-Cepriano
authors.forename: A D
authors.initials: AD
authors.lastname: Montaner
authors.forename: M P
authors.initials: MP
authors.lastname: Schreibman
authors.forename: J E
authors.initials: JE
authors.lastname: Rivier
authors.forename: N M
authors.initials: NM
authors.lastname: Sherwood
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D003532:Cyprinodontiformes / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics* / Q000302:isolation & purification; D008297:Male; D010902:Pituitary Gland / Q000737:chemistry; D023701:Smegmamorpha / Q000378:metabolism*; D014020:Tissue Extracts / Q000737:chemistry
chemical_list: D014020:Tissue Extracts; D007987:Gonadotropin-Releasing Hormone