Asynchronous replication of alleles in genomes carrying a microdeletion.
BACKGROUND
While most allelic pairs of DNA replicate synchronously during the S phase of the cell cycle, some genes normally replicate asynchronously, i.e., genes on the X chromosome and imprinted genes. The replication control mechanism is unknown but was shown to be impaired in malignancies and chromosomal trisomies where replication pattern becomes asynchronous.


OBJECTIVE
To determine the level of asynchronization in replication timing of cells from patients with microdeleted genomes.


METHODS
We applied monocolor fluorescent in situ hybridization with different probes on leukocytes from microdeleted genomes.


RESULTS
All samples derived from the microdeleted genomes showed significantly higher levels of an asynchronized pattern compared to normal individuals.


CONCLUSIONS
Even a "small" genetic imbalance (microdeletion) can interfere with gene replication and cell cycle progression, as previously shown in full trisomies.
Amiel
Aliza
A
Genetic Institute, Meir General Hospital, Kfar Saba, Israel. alizaamiel@hotmail.com
Reish
Orit
O
Gaber
Elena
E
Masterman
Ronit
R
Tohami
Tally
T
Fejgin
Moshe D
MD
D022981:Allelic Imbalance / Q000235:genetics*; D002904:Chromosomes, Human, 21-22 and Y / Q000235:genetics; D004261:DNA Replication / Q000235:genetics*; D017353:Gene Deletion; D016259:Genes, myc / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007962:Leukocytes / Q000502:physiology; D015336:Molecular Probe Techniques; D016160:Retinoblastoma Protein / Q000235:genetics; D016196:S Phase / Q000235:genetics
D016160:Retinoblastoma Protein