[Identification of acid-stable carmine in imported apple syrup product].
An unknown red pigment was purified from an apple syrup product imported from Canada, using a DIAION HP-20 column with methanol as the eluent. By spectroscopic means and chemical synthesis, the isolated pigment was identified as 4-aminocarminic acid, which is the major pigment of acid-stable carmine (a red colorant illegal in Japan). In addition, HPLC and TLC methods were proposed to detect this illegal colorant. While the color of carminic acid changed from yellow to red in the pH range of McIlvaine buffer (3.0-7.0), the color of 4-aminocarminic acid was always red, and also the ultraviolet/visible (UV/Vis) spectra did not change. These characteristics are useful to distinguish 4-aminocarminic acid from carminic acid.
Kawasaki
Yoko
Y
National Institute of Health Sciences: 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Sugimoto
Naoki
N
Sato
Kyoko
K
Yamazaki
Takeshi
T
Ishiwata
Hajimu
H
Maitani
Tamio
T
D002329:Carmine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005504:Food Analysis; D005505:Food Coloring Agents / Q000032:analysis*; D027845:Malus
D005505:Food Coloring Agents; D002329:Carmine