title: Cholesterol oxidation using hollow fiber dialyzer immobilized with cholesterol oxidase: effect of storage and reuse.
abstract: Cholesterol oxidase (COD) was covalently bonded onto the surface of polyacrylonitrile (PAN) hollow fiber via glutaraldehyde. The optimum conditions were found to be at pH 7, -20 degrees C and in the dry state. Immobilized COD retained relative activity above 42% after a 30-day period, when storing at 25 degrees C. After reusing for 30 times, the activity of dry-stored immobilized COD retained 58% of activity. By rinsing with PBS, the decrease in the activity can be greatly reduced. At a flow speed of 1.09cm/s, the immobilized COD can decrease the concentration of cholesterol by about 40% in a 4-h run.
authors.affiliation: Department of Polymer Engineering, Hollow Fiber Laboratory, National Taiwan University of Science and Technology, Taipei 10672, Taiwan.
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Lin
authors.forename: Ming-Chien
authors.initials: MC
authors.lastname: Yang
mesh_terms: D002784:Cholesterol / Q000378:metabolism*; D002789:Cholesterol Oxidase / Q000378:metabolism*; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D006435:Renal Dialysis / Q000295:instrumentation; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D002784:Cholesterol; D002789:Cholesterol Oxidase