Analysis of caprine arthritis encephalitis virus (CAEV) temporal gene expression in infected cells.
Caprine arthritis encephalitis virus (CAEV) is a lentivirus that is closely related to visna virus and more distantly related to the human lentivirus, Human Immunodeficiency Virus type 1 (HIV-1). The CAEV genome contains several small open reading frames (ORFs) that encode viral regulatory proteins. One of these non-structural proteins, Rev-C, is required for cytoplasmic transport of viral un/incompletely spliced mRNAs and efficient viral replication. In HIV-1 and visna virus, Rev is responsible for the temporal shift from non-structural protein synthesis to synthesis of structural proteins that is observed during the viral infectious cycle. Since it encodes a Rev protein, CAEV would be predicted to exhibit a similar temporal shift in gene expression during its replicative cycle. Immunoprecipitation analysis of 35S-pulse labeled, CAEV-infected goat synovial membrane (GSM) cells indicates that Rev-C is more abundant than is Gag at 12 h post-infection (PI); at later times PI Gag predominates. Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) experiments using nuclear and cytoplasmic RNA from CAEV-infected GSM cells indicates that the viral unspliced gag mRNA accumulates significantly in the cytoplasm only after Rev is detected. These data indicate that a temporal shift from viral non-structural to structural gene expression occurs in CAEV infected GSM cells.
Schoborg
Robert V
RV
Department of Microbiology, James H Quillen College of Medicine, East Tennessee State University, Box 70579, Johnson City, TN 37614-0579, USA. schoborg@etsu.edu
D000818:Animals; D016088:Arthritis-Encephalitis Virus, Caprine / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D015683:Gene Products, gag / Q000378:metabolism; D015694:Gene Products, rev / Q000235:genetics / Q000378:metabolism*; D006041:Goats; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
D015683:Gene Products, gag; D015694:Gene Products, rev; D012333:RNA, Messenger; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins