title: Transcription of sterol Delta(5,6)-desaturase and sterol 14alpha-demethylase is induced in the plant pathogenic ascomycete, Leptosphaeria maculans, during treatment with a triazole fungicide.
abstract: Two genes whose derived amino acid sequences closely resemble the ergosterol biosynthetic enzymes, sterol Delta(5,6)-desaturase (erg3) and sterol 14alpha-demethylase (erg11), were cloned from the plant pathogenic fungus Leptosphaeria maculans. Transcript levels of both these genes increased following exposure of L. maculans to the triazole fungicide, flutriafol, which specifically inhibits the ergosterol biosynthetic pathway. This induction may be due to a decrease in ergosterol content or to abnormal levels of the ergosterol precursor, 24-methylene dihydrolanosterol.
authors.affiliation: School of Botany, the University of Melbourne, 3010, Melbourne, Victoria, Australia.
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Griffiths
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Howlett
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D004790:Enzyme Induction; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000096:biosynthesis / Q000235:genetics*; D010944:Plants / Q000382:microbiology; D017386:Sequence Homology, Amino Acid; D058886:Sterol 14-Demethylase; D014158:Transcription, Genetic; D014230:Triazoles / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D014230:Triazoles; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D058886:Sterol 14-Demethylase; C039306:sterol delta-5 desaturase