An improved method for rapid ABO genotyping.
A method for ABO genotyping originally designed by Lee and Chang [3] and further developed by Akane et al. [7] has been even more simplified and improved. We obtained a rapid, robust, sensitive and low cost method for detection of sequence polymorphism of ABO glycosyltransferase gene by changing the MaeII restriction enzyme for its isoschizomer TaiI and by optimization of the condition during digestion and electrophoretic separation.
Vanĕk
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DNA Laboratory, Institute of Criminalistics, Police of Czech Republic, POB 62/KUP, Strojnická 27, 170 82 Prague 7, Czech Republic. danvanek@login.cz
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D000017:ABO Blood-Group System / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005554:Forensic Medicine; D005838:Genotype; D016695:Glycosyltransferases / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D000017:ABO Blood-Group System; D016695:Glycosyltransferases; D004262:DNA Restriction Enzymes