Chromium incorporated in RNA and DNA.
The objective of this study was to examine the effect of Cr(III) (chromium chloride) and Cr(VI) (potassium dichromate) on RNA and DNA-chromium adducts formation in isolated nucleic acids and isolated pig lymphocytes. The incubation of cells with potassium dichromate and chromium chloride at concentrations of 10 and 100 microM results in binding of a 1.2-1.9 fold greater number of chromium atoms to nuclear DNA than to total cellular RNA. The incubation of total cellular RNA and nuclear DNA isolated from lymphocytes with CrCl3 and K2Cr2O7 yielded a binding of 1.1-1.6 fold more of Cr atoms to RNA than to DNA. The number of chromium atoms bound to nucleic acids is higher after incubation with K2Cr2O7 than with CrCl3 in both experimental systems.
Krupa
Renata
R
Department of Molecular Genetics, University of Lódź, Poland.
Stañczak
Marcin
M
Walter
Zofia
Z
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002712:Chlorides / Q000737:chemistry*; D017608:Chromium Compounds / Q000737:chemistry*; D004247:DNA / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D018736:DNA Adducts / Q000097:blood*; D008214:Lymphocytes / Q000737:chemistry; D011192:Potassium Dichromate / Q000737:chemistry*; D013552:Swine
D002712:Chlorides; D017608:Chromium Compounds; D018736:DNA Adducts; D004247:DNA; C017133:chromous chloride; D011192:Potassium Dichromate