[Effect of EMP-1 gene on human esophageal cancer cell line].
OBJECTIVE
EMP-1 was selected from a series of differential expressed genes obtained from cDNA microarray in the authors' lab. Epithelial membrane pnteiu-1 gene (EMP-1) was expressed 6 fold lower in esophageal cancer than in normal tissue. The authors further designed the experiment to study the effect of human EMP-1 gene on human esophageal cancer cell line in order to explain the function of this gene on the carcinogensis and progression esophageal cancer.


METHODS
EMP-1 gene was cloned into eukaryotic vector and transfected into the human esophageal cancer cell line. The transfection effect was qualified by Western blot and RT-PCR method. The cell growth curve was observed and the cell cycle was checked by FACS method.


RESULTS
EMP-1 was transfected into EC9706 cell line and its expression was up-regulated. The cell growth is accelerated and expression of EMP-1 is linked to induction of S phase arrest.


CONCLUSIONS
EMP-1 gene has some relationship with carcinogenesis of esophagus.
Wang
Hai-tao
HT
National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100021, P. R. China.
Liu
Zhi-hua
ZH
Wang
Xiu-qin
XQ
Wu
Min
M
D015153:Blotting, Western; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006801:Humans; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014162:Transfection; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D015800:Protozoan Proteins; C085731:erythrocyte membrane protein 1, Plasmodium falciparum