Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype Reston by the use of recombinant nucleoprotein-expressing HeLa cells.
An indirect immunofluorescent assay (IFA) to detect Ebola virus subtype Reston (EBO-R) antibodies was developed by the use of a HeLa cell line stably expressing EBO-R nucleoprotein (NP). This IFA has a high specificity for the detection of EBO-R IgG antibodies in both hyperimmune rabbit sera and monkey sera collected during an EBO-R outbreak in the Philippines in 1996. Furthermore, this IFA showed a higher sensitivity for the detection of EBO-R antibodies than did the IFA using HeLa cells expressing the NP of Ebola virus subtype Zaire. These results suggest that this new IFA is useful for seroepidemiological studies of EBO-R infection among monkeys.
Ikegami
Tetsuro
T
Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
Saijo
Masayuki
M
Niikura
Masahiro
M
Miranda
Mary E G
ME
Calaor
Alan B
AB
Hernandez
Marvin
M
Manalo
Daria L
DL
Kurane
Ichiro
I
Yoshikawa
Yasuhiro
Y
Morikawa
Shigeru
S
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D029043:Ebolavirus / Q000276:immunology* / Q000302:isolation & purification; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006367:HeLa Cells; D019142:Hemorrhagic Fever, Ebola / Q000276:immunology; D006801:Humans; D008252:Macaca fascicularis; D008992:Monkey Diseases / Q000821:virology; D009698:Nucleoproteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D011817:Rabbits; D011994:Recombinant Proteins / Q000378:metabolism; D012680:Sensitivity and Specificity; D014162:Transfection / Q000379:methods
D000914:Antibodies, Viral; D009698:Nucleoproteins; D011994:Recombinant Proteins