title: Simultaneous determination of the novel neuroprotective agent KR-31378 and its metabolite KR-31612 using high performance liquid chromatography with tandem mass spectrometry in human plasma.
abstract: An LC/MS/MS method for the simultaneous determination of a neuroprotective agent for ischemia-reperfusion damage, KR-31378 and its N-acetyl metabolite KR-31612 in human plasma was developed. KR-31378, KR-31612 and the internal standard, KR-31543 were extracted from human plasma by liquid-liquid extraction. A reverse-phase HPLC separation was performed on Luna phenylhexyl column with the mixture of acetonitrile-5 mM ammonium formate (55:45, v/v) as mobile phase. The detection of analytes was performed using an electrospray ionization tandem mass spectrometry in the multiple reaction monitoring mode. The lower limits of quantification for KR-31378 and KR-31612 were 2.0 ng/ml. The method showed a satisfactory sensitivity, precision, accuracy, recovery and selectivity.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada.
authors.forename: John
authors.initials: J
authors.lastname: Ki
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Ji
authors.forename: Sung-Eun
authors.initials: SE
authors.lastname: Yoo
authors.forename: Sun Ok
authors.initials: SO
authors.lastname: Kim
authors.forename: Dong Ha
authors.initials: DH
authors.lastname: Lee
authors.forename: Hong
authors.initials: H
authors.lastname: Lim
authors.forename: Hye Suk
authors.initials: HS
authors.lastname: Lee
mesh_terms: D001578:Benzopyrans / Q000097:blood* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006146:Guanidines / Q000097:blood* / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D018696:Neuroprotective Agents / Q000097:blood* / Q000737:chemistry; D011714:Pyrans / Q000097:blood* / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D001578:Benzopyrans; D006146:Guanidines; C473250:KR-31612; C438686:N''-cyano-N-(6-amino-3,4-dihydro-3-hydroxy-2-methyl-2-dimethoxymethyl-2H-benzopyran-4-yl)-N'-benzylguanidine; D018696:Neuroprotective Agents; D011714:Pyrans