Enzymatic preparation of D-beta-acetylthioisobutyric acid and cetraxate hydrochloride using a stereo- and/or regioselective hydrolase, 3,4-dihydrocoumarin hydrolase from Acinetobacter calcoaceticus.
3,4-Dihydrocoumarin hydrolase (DCH) from Acinetobacter calcoaceticus F46, which was previously found on screening for aromatic lactone-hydrolyzing enzymes, catalyzes the hydrolysis of several linear esters. The substrate specificity of the enzyme toward linear esters was quite characteristic, i.e., (1) it was specific toward methyl esters, (2) it recognized the configuration at the 2-position, and (3) it hydrolyzed diesters to monoesters. DCH hydrolyzed the methyl esters of beta-acetylthioisobutyrate and cetraxate. The products of these reactions were identified as D-beta-acetylthioisobutyrate and cetraxate, respectively, i.e., the hydrolysis reactions catalyzed by DCH were stereo- and/or regioselective. With recombinant Escherichia coli cells expressing the DCH gene as a catalyst, stereospecific hydrolysis of methyl beta-acetylthioisobutyrate and regioselective hydrolysis of methyl cetraxate proceeded efficiently.
Honda
K
K
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Kataoka
M
M
Shimizu
S
S
D016954:Acinetobacter calcoaceticus / Q000378:metabolism*; D002087:Butyrates / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008968:Molecular Conformation; D013379:Substrate Specificity; D014148:Tranexamic Acid / Q000031:analogs & derivatives* / Q000378:metabolism*
D002087:Butyrates; D006867:Hydrolases; D014148:Tranexamic Acid; C090217:3-acetylthioisobutyric acid; C000665:cetraxate; C403749:3,4-dihydrocoumarin hydrolase