title: A cryopreservation protocol for embryos of the endangered species Zizania texana.
abstract: Seeds of the endangered species Zizania texana are recalcitrant, making it difficult to preserve the remaining genetic diversity of this species in genebanks. Excised embryos can be cryopreserved using solution-based cryoprotection protocols. Survival following cryoexposure increased from less than 5% to about 75% by preculturing embryos in high concentrations of sugars, bathing them in cryoprotectant solutions, and partially drying them to water contents of about 0.6 g H2O/g dry mass.
authors.affiliation: USDA-ARS National Center for Genetic Resources, 1111 S. Mason Street, Fort Collins, Colorado, USA. chrisv@lamar.colostate.edu
authors.forename: Christina
authors.initials: C
authors.lastname: Walters
authors.forename: Darren H
authors.initials: DH
authors.lastname: Touchell
authors.forename: Paula
authors.initials: P
authors.lastname: Power
authors.forename: James
authors.initials: J
authors.lastname: Wesley-Smith
authors.forename: Michael F
authors.initials: MF
authors.lastname: Antolin
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D003890:Desiccation / Q000379:methods*; D006109:Poaceae / Q000196:embryology*; D012639:Seeds / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development*; D015996:Survival Rate; D013997:Time Factors; D014867:Water / Q000032:analysis
chemical_list: D003451:Cryoprotective Agents; D014867:Water