title: Caveolin-1 redistribution in human endothelial cells induced by laminar flow and cytokine.
abstract: Caveolin-1 is a principal component of caveolae and is involved in signaling transduction in a number of cells. A hypothesis was proposed in this work that mechanical forces due to flow induce caveolin-1 translocation. So the changes of caveolin-1 expression and distribution in cultured endothelial cells (HUVECs) exposed to a steady laminar flow were studied. For comparing with the influence of cytokine, caveolin-1 in the cells stimulated by TNF-alpha was also investigated. Indirect immunofluorescence and double fluorescence labeling showed that in control cells, caveolin-1 was primarily localized on the cell surface, which corresponded to the peripheral distribution of F-actin, and presented some local concentrations. In the cells exposed to a laminar flow (1.0 Pa), caveolin-1 distribution showed a time-dependent variation. After 24 h of shear, the local concentration of caveolin-1 was found, in the most cells, at upstream side of cell body. Also more caveolin-1 molecules were observed in the cells. In contrast, TNF-alpha induced a decrease of caveolin-1 in cells. The redistribution of caveolin-1 seems to be correlated to F-actin organization.
authors.affiliation: Cell and Tissue Engineering and Mechanics, LEMTA-UMR 7563 CNRS/INPL/UHP and IFR 111 Bioengineering, 2 avenue de la Forêt de Haye, 54500 Vandoeuvre-lès-Nancy, France.
authors.forename: R J
authors.initials: RJ
authors.lastname: Sun
authors.forename: S
authors.initials: S
authors.lastname: Muller
authors.forename: F Y
authors.initials: FY
authors.lastname: Zhuang
authors.forename: J F
authors.initials: JF
authors.lastname: Stoltz
authors.forename: X
authors.initials: X
authors.lastname: Wang
mesh_terms: D000199:Actins / Q000378:metabolism; D051242:Caveolin 1; D022461:Caveolins / Q000378:metabolism*; D018929:Cell Culture Techniques; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D018056:Hemorheology; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008856:Microscopy, Fluorescence; D013314:Stress, Mechanical; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D000199:Actins; C495540:CAV1 protein, human; D051242:Caveolin 1; D022461:Caveolins; D014409:Tumor Necrosis Factor-alpha