Regulation of the pdx1 gene promoter in pancreatic beta-cells.
In the adult pancreas the expression of the transcription factor PDX1 is mainly restricted to the beta-cells of the islets of Langerhans. In this study we have identified a region of the pdx1 promoter between -2715 and -1960 which was essential to direct pancreatic islet-cell-specific expression of PDX1. We have also begun for the first time to understand the complex nutritional and hormonal regulation controlling PDX1 expression. The current study has established the fact that glucose, GLP-1, insulin, T(3), HB-EGF, and TNF-alpha all positively regulate the PDX1 gene promoter in pancreatic beta-cells. This study represents the first detailed exploration of the nutritional and hormonal regulation of this vital beta-cell gene.
Campbell
Susan C
SC
School of Cell and Molecular Biosciences, University of Newcastle upon Tyne, NE2 4HH, Newcastle upon Tyne, UK.
Macfarlane
Wendy M
WM
D000818:Animals; D002460:Cell Line; D005934:Glucagon / Q000494:pharmacology; D052216:Glucagon-Like Peptide 1; D005947:Glucose / Q000494:pharmacology; D018398:Homeodomain Proteins; D006728:Hormones / Q000494:pharmacology; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D010446:Peptide Fragments / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011498:Protein Precursors / Q000494:pharmacology; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion; D015534:Trans-Activators / Q000235:genetics*; D015533:Transcriptional Activation
D018398:Homeodomain Proteins; D006728:Hormones; D010446:Peptide Fragments; D011498:Protein Precursors; D015534:Trans-Activators; C084010:pancreatic and duodenal homeobox 1 protein; D052216:Glucagon-Like Peptide 1; D005934:Glucagon; D005947:Glucose