title: Immunohistochemical detection of cyclooxygenase-2 in lungs of pigs naturally infected with Actinobacillus pleuropneumoniae.
abstract: Cyclooxygenase-2 (COX-2) protein was detected immunohistochemically in formalin-fixed, paraffin wax-embedded lung tissues from 15 pigs with naturally occurring pleuropneumonia caused by Actinobacillus pleuropneumoniae. Positive cells typically exhibited a red reaction product without background staining. Alveolar macrophages, neutrophils, and bronchial and bronchiolar epithelial cells had positive immunohistochemical signals. Immunoreactivity of COX-2 protein was intense in the clustered leucocytes with streaming nuclear chromatin that are a characteristic histological feature of porcine pleuropneumonia. COX-2 protein was always associated with macrophages and neutrophils in pleuropneumonic lung lesions but was minimal in non-lesional lung of A. pleuropneumoniae -infected pigs and in normal lung from control pigs. The results suggest that COX-2 plays a role in pathophysiological processes during A. pleuropneumoniae infection.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Kyounggi-Do, Republic of Korea.
authors.forename: W-S
authors.initials: WS
authors.lastname: Cho
authors.forename: C
authors.initials: C
authors.lastname: Chae
mesh_terms: D000189:Actinobacillus Infections / Q000201:enzymology / Q000473:pathology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000472:pathogenicity / Q000502:physiology*; D000818:Animals; D051546:Cyclooxygenase 2; D007124:Immunoenzyme Techniques / Q000379:methods / Q000662:veterinary*; D007527:Isoenzymes / Q000378:metabolism*; D008168:Lung / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D013552:Swine; D013553:Swine Diseases / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology
chemical_list: D007527:Isoenzymes; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases