[A study on gene expression of angiotensin receptors in arteries from tail-suspended rats].
Objective. To investigate whether simulated microgravity [correction of microgravtiy] can result in alterations of mRNA expression of angiotensin receptors in rat arteries. Method. Rats were divided into simulated microgravity group and synchronous control group. Simulated microgravity rats were subjected to tail-suspension for 4-wk to simulate the effect of weightlessness. Result. Results from RT-PCR demonstrated that mRNAs of angiotensin II receptors, AT1a, AT1b, and AT2 were expressed in carotid arteries, abdominal aorta and femoral arteries, whereas only mRNAs of AT1a and AT1b were expressed in basilar arteries. There were no significant differences between the simulated-microgravity and control rats in the expression of mRNAs of AT1a, AT1b, and AT2 in these arteries. Conclusion. Angiotensin II receptors may play no regulatory role in the occurrence of the differentiated vascular adaptation during simulated microgravity.
Zhang
Le-ning
LN
Department of Aerospace Physiology, The Fourth Military Medical University, Xi'an. zhangln@fmmu.edu.cn
Meng
Qing-jun
QJ
Zhang
Li-fan
LF
Ma
Jin
J
D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D001012:Aorta, Abdominal / Q000502:physiology; D001158:Arteries / Q000378:metabolism* / Q000502:physiology; D001488:Basilar Artery / Q000502:physiology; D002339:Carotid Arteries / Q000502:physiology; D005263:Femoral Artery / Q000502:physiology; D015870:Gene Expression; D019417:Hindlimb Suspension; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; D011945:Receptors, Angiotensin / Q000235:genetics / Q000378:metabolism*; D012084:Renin-Angiotensin System / Q000235:genetics; D018474:Weightlessness Simulation
D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; D011945:Receptors, Angiotensin