[Platelet rich plasma (PRP): potentialities and techniques of extraction].
This paper describes the various techniques of platelet-rich plasma (PRP) extraction codified in recent years and their use potential is evaluated. PRP is one of the techniques with which at the moment it is attempted to modulate and facilitate the cure of a wound. The use of PRP is based on the theoretical premise that by concentrating platelets the effects of the growth factors (PDGF, TGF-beta, IGF-I and -II) so released will be increased. Marx's original technique is described above all. This prescribes the sampling of a unit of blood (450-500 ml) and the use of a cell separator. We then analysed the technique of Marx and Hannon in which the quantity of blood sampled is reduced to 150 ml, and the two simplified techniques of the Sacchi and Bellanda group. Finally, a new PRP extraction technique is described. We conclude that platelet gel allows access to autologous growth factors which by definition are neither toxic nor immunogenic and are capable of accelerating the normal processes of bone regeneration. PRP can thus be considered a useful instrument for increasing the quality and final quantity of regenerated bone in oral and maxillo-facial surgery operations.
Pacifici
L
L
Cattedra di Clinica Odontostomatologica, Corso di Laurea in Odontoiatria e Protesi Dentaria, Università degli Studi di Roma La Sapienza, Rome, Italy. dr.pacifici@libero.it
Casella
F
F
Maggiore
C
C
D001688:Biological Products / Q000302:isolation & purification / Q000627:therapeutic use; D001792:Blood Platelets / Q000378:metabolism; D001861:Bone Regeneration / Q000187:drug effects; D002122:Calcium Chloride / Q000494:pharmacology; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D002498:Centrifugation; D004798:Enzymes / Q000494:pharmacology; D005340:Fibrinogen / Q000737:chemistry; D005782:Gels; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism / Q000494:pharmacology; D007335:Insulin-Like Growth Factor II / Q000378:metabolism / Q000494:pharmacology; D019647:Oral Surgical Procedures; D010949:Plasma / Q000737:chemistry / Q000166:cytology*; D010976:Platelet Count; D010982:Platelet-Derived Growth Factor / Q000378:metabolism / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000378:metabolism / Q000494:pharmacology
D001688:Biological Products; D004798:Enzymes; D005782:Gels; D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta; C064929:botropase; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D005340:Fibrinogen; D002122:Calcium Chloride