A cryopreservation protocol for embryos of the endangered species Zizania texana.
Seeds of the endangered species Zizania texana are recalcitrant, making it difficult to preserve the remaining genetic diversity of this species in genebanks. Excised embryos can be cryopreserved using solution-based cryoprotection protocols. Survival following cryoexposure increased from less than 5% to about 75% by preculturing embryos in high concentrations of sugars, bathing them in cryoprotectant solutions, and partially drying them to water contents of about 0.6 g H2O/g dry mass.
Walters
Christina
C
USDA-ARS National Center for Genetic Resources, 1111 S. Mason Street, Fort Collins, Colorado, USA. chrisv@lamar.colostate.edu
Touchell
Darren H
DH
Power
Paula
P
Wesley-Smith
James
J
Antolin
Michael F
MF
D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D003890:Desiccation / Q000379:methods*; D006109:Poaceae / Q000196:embryology*; D012639:Seeds / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development*; D015996:Survival Rate; D013997:Time Factors; D014867:Water / Q000032:analysis
D003451:Cryoprotective Agents; D014867:Water