Characterization of a toxigenic Vibrio cholerae O139 strain belonging to a new ribotype and isolated from a diarrheal patient.
We characterized a Vibrio cholerae O139 strain isolated from a diarrheal patient admitted to Taluk Hospital, Cherthala, Alleppey, Kerala, India, on 9 June 2000. The V. cholerae O139 strain possesses the core of the CTX genetic element, colonization toxin-coregulated pilus, the adherence outer membrane protein, and the central regulatory protein encoded by toxR and produces cholera toxin (200 pg/ml). We provide molecular evidence showing that toxigenic V. cholerae O139 strain ALO95 belongs to a distinct genotype characterized by a unique ribotype designated B-VII and has a unique enterobacterial repetitive intergenic consensus sequence PCR fingerprint profile designated E-V.
Bhanumathi
R
R
Rajiv Gandhi Center for Biotechnology, Poojappura, Thiruvananthapuram 695 014, Kerala, India.
Sabeena
F
F
Isac
Sree Renjini
SR
Radhakutty
G
G
Singh
D V
DV
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D002771:Cholera / Q000382:microbiology; D002772:Cholera Toxin / Q000235:genetics* / Q000378:metabolism; D003967:Diarrhea / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D021521:Ribotyping; D041242:Vibrio cholerae O139 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D014774:Virulence
D001426:Bacterial Proteins; D002772:Cholera Toxin