title: alphaNAC requires an interaction with c-Jun to exert its transcriptional coactivation.
abstract: alphaNAC is a transcriptional coactivator known to interact with the N-terminal activation domain of the c-Jun transcription factor. In this article, we describe the identification of the c-Jun interaction domain within the alphaNAC protein. Deletion analysis of alphaNAC indicated that the c-Jun binding site was located in the middle part of the protein, between residues 89 and 129. The deletion of the C-terminal end of alphaNAC, including the c-Jun interacting domain, induced a nuclear translocation of the mutated coactivator. Despite its presence in the nucleus, this deletion mutant did not retain the capacity to coactivate an AP-1 response. These results demonstrate that the interaction between alphaNAC and c-Jun was necessary for the potentiation of theAP-1 transcriptional activity. These data are consistent with a mechanism by which alphaNAC acts as a coactivator for c-Jun-dependent transcription by interacting with the c-Jun N-terminal activation domain.
authors.affiliation: Genetics Unit, Shriners Hospital for Children, McGill University, Montreal, Quebec, Canada.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Quèlo
authors.forename: Mélanie
authors.initials: M
authors.lastname: Hurtubise
authors.forename: René
authors.initials: R
authors.lastname: St-Arnaud
mesh_terms: D000818:Animals; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism; D002845:Chromatography; D017353:Gene Deletion; D005822:Genetic Vectors; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008156:Luciferases / Q000378:metabolism; D018832:Molecular Chaperones; D009154:Mutation; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016755:Proto-Oncogene Proteins c-jun / Q000737:chemistry / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013997:Time Factors; D015534:Trans-Activators / Q000737:chemistry* / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection
chemical_list: D018832:Molecular Chaperones; D016755:Proto-Oncogene Proteins c-jun; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; C096099:nascent-polypeptide-associated complex; D008156:Luciferases