Genotyping single-nucleotide polymorphisms by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry.
In recent years matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI) has emerged as a very powerful method for genotyping single nucleotide polymorphisms. The accuracy, speed of data accumulation, and data structure are the major features of MALDI. Several SNP genotyping methods have been implemented with a high degree of automation and are being applied for large-scale association studies. Most methods for SNP genotyping using MALDI mass spectrometric detection and their potential application for high-throughput are reviewed here.
Sauer
Sascha
S
Max-Planck-Institut für Molekulare Genetik, Abteilung Lehrach, Ihnestrasse 73, 14195 Berlin-Dahlem, Germany.
Gut
Ivo G
IG
D001331:Automation; D005838:Genotype; D009693:Nucleic Acid Hybridization; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*