title: High-throughput assay of ( R)-phenylacetylcarbinol synthesized by pyruvate decarboxylase.
abstract: A novel assay has been developed for the detection of ( R)-phenylacetylcarbinol, ( R)-PAC, a chiral intermediate in the industrial synthesis of ephedrine. It is the product of a biotransformation of benzaldehyde catalysed by the enzyme pyruvate decarboxylase. The assay, using 2,3,5-triphenyltetrazolium chloride, enables high-throughput photometric analysis of the activity of the enzyme thus avoiding time-consuming chromatographic procedures.
authors.affiliation: BASF AG, GVF/E-A030, 67056 Ludwigshafen, Germany. Michael.Breuer@basf-ag.de
authors.forename: Michael
authors.initials: M
authors.lastname: Breuer
authors.forename: Martina
authors.initials: M
authors.lastname: Pohl
authors.forename: Bernhard
authors.initials: B
authors.lastname: Hauer
authors.forename: Bettina
authors.initials: B
authors.lastname: Lingen
mesh_terms: D000096:Acetone / Q000031:analogs & derivatives* / Q000032:analysis* / Q000138:chemical synthesis; D010783:Photometry / Q000379:methods*; D011767:Pyruvate Decarboxylase / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D000096:Acetone; C035293:1-hydroxy-1-phenyl-2-propanone; D011767:Pyruvate Decarboxylase