title: Effects of dark rearing on phosphorylation of neurotrophin Trk receptors.
abstract: Total lack of visual experience (dark rearing, DR) is known to affect development of mammalian visual cortex (VC) and to prolong the critical period of visual cortical plasticity. Neurotrophins (NTs) have been proposed to play a relevant role in activity dependent processes important for the final shaping of cortical visual connections. Neurotrophin supply or antagonism of endogenous NT action profoundly affect visual cortical development and plasticity; in particular, exogenous supply of NTs counteracts DR effects on VC development. However, the effects of DR on NT expression are still debated and mounting evidence reports a mismatch between BDNF mRNA and protein expression in DR animals. To gain insight into the effects of DR on expression of nerve growth factor (NGF) and the functional state of NT signalling pathways, we assessed the phosphorylation state of Trk receptors in light-reared animals (LR), in dark-reared animals (DR), in DR animals briefly exposed to light and in DR animals with exogenous supply of NTs [NGF, brain-derived neurotrophic factor (BDNF) and NT-4] in the VC. We report that DR increases the expression of NGF but reduces the phosphorylation of TrkA and TrkB receptors with respect to LR; normal phosphorylation is rapidly rescued by a brief exposure to light. Exogenous supply of NGF, BDNF or NT4 in DR animals also rescues the phosphorylation of their receptors.
authors.affiliation: Scuola Normale Superiore, p.zza Dei Cavalieri, 7, 56126, Pisa, Italy. viegi@in.pi.cnr.it
authors.forename: Alessandro
authors.initials: A
authors.lastname: Viegi
authors.forename: Tiziana
authors.initials: T
authors.lastname: Cotrufo
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Berardi
authors.forename: Laura
authors.initials: L
authors.lastname: Mascia
authors.forename: Lamberto
authors.initials: L
authors.lastname: Maffei
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology; D003624:Darkness / Q000009:adverse effects*; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D020932:Nerve Growth Factor / Q000378:metabolism* / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D010775:Photic Stimulation; D011233:Precipitin Tests; D051381:Rats; D020318:Rats, Long-Evans; D020917:Receptor, trkA / Q000187:drug effects / Q000378:metabolism*; D020813:Receptor, trkB / Q000187:drug effects / Q000378:metabolism*; D012683:Sensory Deprivation; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D015854:Up-Regulation / Q000187:drug effects; D014793:Visual Cortex / Q000187:drug effects / Q000378:metabolism*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D020932:Nerve Growth Factor; D020917:Receptor, trkA; D020813:Receptor, trkB; C073922:neurotrophin 4