title: Cerebrospinal fluid and serum protein levels of tumour necrosis factor-alpha (TNF-alpha) interleukin-6 (IL-6) and soluble interleukin-6 receptor (sIL-6R gp80) in multiple sclerosis patients.
abstract: The aim of this study was to evaluate soluble proteins of tumour necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and IL-6 receptor subunit gp80 (sIL-6R gp80), as markers of multiple sclerosis (MS). Paired cerebrospinal fluid (CSF) and serum samples of 20 MS patients and 15 controls suffering from non-inflammatory neurological diseases have been assayed retrospectively using monoclonal antibodies-based ELISAs. While TNF-alpha could not be detected in CSF, it was measurable in 20% of total sera. Interleukin-6 was measurable in 5% of total CSF and in 10% of total sera only. However, soluble IL-6R gp80 protein subunit was readily measurable, showing sera concentration (pg/mL) about 34 times higher and specific content (pg/mg total protein) around five times lower than those in paired CSF, similarly for both group of patients. No significant difference of sIL-6R gp80 level, which could be disease-, gender- or age-related, and no correlation of CSF sIL-6R gp80 content with that of paired serum or with routine clinical data for CSF, have been observed. We have concluded that soluble proteins of TNF-alpha, IL-6 and sIL-6R gp80 assayed by monoclonal antibodies-based ELISAs could not serve as markers of the MS activity.
authors.affiliation: Department of Neurology, General Hospital Sveti Duh, Zagreb, Croatia.
authors.forename: Anton
authors.initials: A
authors.lastname: Vladić
authors.forename: Gordana
authors.initials: G
authors.lastname: Horvat
authors.forename: Stjepan
authors.initials: S
authors.lastname: Vukadin
authors.forename: Zvonimir
authors.initials: Z
authors.lastname: Sucić
authors.forename: Sumski
authors.initials: S
authors.lastname: Simaga
mesh_terms: D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood / Q000134:cerebrospinal fluid*; D008297:Male; D009103:Multiple Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid / Q000209:etiology / Q000276:immunology*; D019947:Receptors, Interleukin-6 / Q000097:blood*; D012995:Solubility; D014409:Tumor Necrosis Factor-alpha / Q000134:cerebrospinal fluid* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators; D015850:Interleukin-6; D019947:Receptors, Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C113250:interleukin-6 receptor alpha