Citicoline has a protective effect on damaged retinal ganglion cells in mouse culture retina.
Some clinical reports indicate that exogenous CDP-choline (citicoline) may have a therapeutic effect in patients with glaucoma. However, the precise effect of citicoline on damaged retinal ganglion cells (RGCs) remains to be explained. We performed tissue culture of mouse retinal explants and investigated the effect of citicoline on damaged RGCs by the quantitative analysis of TdT-dUTP terminal nick-end labeling (TUNEL) staining and the assessment of the number of regenerating neurites. The TUNEL-positive ratio in 0.1-10 micromol/l citicoline-treated retina was very low, and the number of regenerating neurites increased more than in control retina. Our findings suggest that citicoline has a protective effect on damaged RGCs in tissue culture of retina.
Oshitari
Toshiyuki
T
Department of Ophthalmology and Visual Science, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba 260-8670, Japan. tarii@aol.com
Fujimoto
Naoya
N
Adachi-Usami
Emiko
E
D000818:Animals; D046508:Culture Techniques; D003566:Cytidine Diphosphate Choline / Q000378:metabolism* / Q000494:pharmacology; D020287:In Situ Nick-End Labeling; D051379:Mice; D008810:Mice, Inbred C57BL; D009416:Nerve Regeneration / Q000187:drug effects; D016501:Neurites / Q000378:metabolism; D018697:Nootropic Agents / Q000378:metabolism* / Q000494:pharmacology; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000378:metabolism*
D018697:Nootropic Agents; D003566:Cytidine Diphosphate Choline