title: Spatial accuracy of 3D reconstructed radioluminographs of serial tissue sections and resultant absorbed dose estimates.
abstract: Many agents using tumour-associated characteristics are deposited heterogeneously within tumour tissue. Consequently, tumour heterogeneity should be addressed when obtaining information on tumour biology or relating absorbed radiation dose to biological effect. We present a technique that enables radioluminographs of serial tumour sections to be reconstructed using automated computerized techniques, resulting in a three-dimensional map of the dose-rate distribution of a radiolabelled antibody. The purpose of this study is to assess the reconstruction accuracy. Furthermore, we estimate the potential error resulting from registration misalignment, for a range of beta-emitting radionuclides. We compare the actual dose-rate distribution with that obtained from the same activity distribution but with manually defined translational and rotational shifts. As expected, the error produced with the short-range 14C is much larger than that for the longer range 90Y; similarly values for the medium range 131I are between the two. Thus, the impact of registration inaccuracies is greater for short-range sources.
authors.affiliation: Department of Oncology, Royal Free and University College Medical School, London, UK.
authors.forename: I A
authors.initials: IA
authors.lastname: Petrie
authors.forename: A A
authors.initials: AA
authors.lastname: Flynn
authors.forename: R B
authors.initials: RB
authors.lastname: Pedley
authors.forename: A J
authors.initials: AJ
authors.lastname: Green
authors.forename: E
authors.initials: E
authors.lastname: El-Emir
authors.forename: J L J
authors.initials: JL
authors.lastname: Dearling
authors.forename: G M
authors.initials: GM
authors.lastname: Boxer
authors.forename: R
authors.initials: R
authors.lastname: Boden
authors.forename: R H J
authors.initials: RH
authors.lastname: Begent
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000532:radiotherapy; D000327:Adsorption; D019412:Anatomy, Cross-Sectional / Q000379:methods*; D000818:Animals; D001345:Autoradiography / Q000379:methods; D015179:Colorectal Neoplasms / Q000473:pathology / Q000532:radiotherapy*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007668:Kidney / Q000166:cytology / Q000528:radiation effects*; D051379:Mice; D009368:Neoplasm Transplantation; D025341:Principal Component Analysis; D011829:Radiation Dosage; D016499:Radioimmunotherapy / Q000379:methods; D011874:Radiometry / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique