New serological assay for detection of putative Helicobacter pylori virulence factors.
We evaluated a new immunoblot assay (Helicoblot 2.1) for Helicobacter pylori infection and CagA and VacA status by using serum samples from 222 patients. The test accurately detected H. pylori infection and VacA status, but improvements in the interpretation criteria are required before it can be recommended for determination of CagA status.
Park
Chang-Young
CY
Department of Medicine, Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Texas, USA.
Cho
Yong-Kyun
YK
Kodama
Tadashi
T
El-Zimaity
Hala M T
HM
Osato
Michael S
MS
Graham
David Y
DY
Yamaoka
Yoshio
Y
D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000032:analysis*; D016481:Helicobacter Infections / Q000276:immunology; D016480:Helicobacter pylori / Q000472:pathogenicity*; D006801:Humans; D015151:Immunoblotting; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014774:Virulence; D037521:Virulence Factors / Q000032:analysis*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; D037521:Virulence Factors; C080866:cagA protein, Helicobacter pylori