Proliferative activity and expression of cyclin-dependent kinase inhibitor p21WAF1 and p53 protein in endothelial cells of human aorta during replicative aging in vitro.
Experiments with bromodeoxyuridine showed that the count of nonproliferating cells in a monolayer culture of aortic endothelial cells from adult humans rapidly increased during long-term subculturing. Cytochemical assay showed that these cells contain neutral b-galactosidase, the marker of aging cells. Immunocytochemical assay demonstrated that most cells express p53 protein and inhibitor of the cell cycle p21WAF1.
Antropova
Yu G
YG
Laboratory of Molecular and Cellular Cardiology, Institute of Experimental Cardiology, Russian Research-and-Production Center for Cardiology, Russian Ministry of Health, Moscow.
Bryantsev
A L
AL
Kalinina
N I
NI
Il'inskaya
O P
OP
Tararak
E M
EM
D000328:Adult; D000375:Aging; D001011:Aorta / Q000166:cytology / Q000378:metabolism*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D017382:Reactive Oxygen Species; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*; D001616:beta-Galactosidase / Q000378:metabolism
C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; D001616:beta-Galactosidase; D001973:Bromodeoxyuridine