title: Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus.
abstract: RNA silencing or post-transcriptional gene silencing (PTGS) in plants is known as a defense system against virus infection. Several plant viruses have been shown to encode an RNA silencing suppressor. Using a green fluorescent protein-based transient suppression assay, we show that NSs protein of Tomato spotted wilt virus (TSWV) has RNA silencing suppressor activity. TSWV NSs protein suppressed sense transgene-induced PTGS but did not suppress inverted repeat transgene-induced PTGS. TSWV NSs protein is the first RNA silencing suppressor identified in negative-strand RNA viruses.
authors.affiliation: Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takeda
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Sugiyama
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nagano
authors.forename: Masashi
authors.initials: M
authors.lastname: Mori
authors.forename: Masanori
authors.initials: M
authors.lastname: Kaido
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Mise
authors.forename: Shinya
authors.initials: S
authors.lastname: Tsuda
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Okuno
mesh_terms: D018506:Gene Expression Regulation, Plant; D008957:Models, Genetic; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid; D014026:Nicotiana / Q000033:anatomy & histology / Q000235:genetics / Q000821:virology; D017865:Tospovirus / Q000472:pathogenicity*; D014764:Viral Proteins / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; D018749:RNA, Plant; D014764:Viral Proteins