title: Characterization of microcapsules: recommended methods based on round-robin testing.
abstract: Alginate beads, as well as microcapsules based on alginate, cellulose sulphate and polymethylene-co-guanidine, were produced at diameters of 0.4, 1.0 and 1.5 mm. These standard materials were tested, by independent laboratories, in regards to water activity, bead or capsule size, mechanical resistance and transport behaviour. The water activity and mechanical resistance were observed to increase with bead and capsule size. Transport properties (ingress) were assessed using a variety of low molar mass and macromolecular probes. It was observed that the penetration of Vitamin B12 increased with bead diameter, as did dextran penetration. However, for the membrane-containing microcapsules, larger membrane thickness, observed for the larger capsules, retarded ingress. The authors, who are part of a European working group, recommend that permeability be assessed either using a large range of probes or a broad molar mass standard, with measurements at one or two molar masses insufficient to simulate the behaviour in application. Mechanical compression is seen as a good means to estimate elasticity and rupture of beads and capsules, with the sensitivity of the force transducer, which can vary from microN to tens of N, required to be tuned to the anticipated bead or capsule strength. Overall, with the exception of the mechanical properties, the precision in the inter-laboratory testing was good. Furthermore, the various methods of assessing transport properties agreed, in ranking, for the beads and capsules characterized, with gels having smaller radii being less permeable. For microcapsules, the permeation across the membrane dominates the ingress, and thicker membranes have lower permeability.
authors.affiliation: Institute of Biocybernetics and Biomedical Engineering, Warsaw, Poland.
authors.forename: S
authors.initials: S
authors.lastname: Rosiński
authors.forename: G
authors.initials: G
authors.lastname: Grigorescu
authors.forename: D
authors.initials: D
authors.lastname: Lewińska
authors.forename: L G
authors.initials: LG
authors.lastname: Ritzén
authors.forename: H
authors.initials: H
authors.lastname: Viernstein
authors.forename: E
authors.initials: E
authors.lastname: Teunou
authors.forename: D
authors.initials: D
authors.lastname: Poncelet
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Fan
authors.forename: D
authors.initials: D
authors.lastname: Serp
authors.forename: I
authors.initials: I
authors.lastname: Marison
authors.forename: D
authors.initials: D
authors.lastname: Hunkeler
mesh_terms: D000464:Alginates / Q000138:chemical synthesis / Q000737:chemistry; D001696:Biomechanical Phenomena; D002214:Capsules / Q000138:chemical synthesis / Q000737:chemistry*; D003911:Dextrans; D004339:Drug Compounding; D005782:Gels; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D066298:In Vitro Techniques; D010316:Particle Size; D010539:Permeability; D013499:Surface Properties; D014805:Vitamin B 12 / Q000008:administration & dosage / Q000493:pharmacokinetics; D014867:Water
chemical_list: D000464:Alginates; D002214:Capsules; D003911:Dextrans; D005782:Gels; D006603:Hexuronic Acids; D014867:Water; D020723:Glucuronic Acid; D014805:Vitamin B 12