Structural characterization of the mouse high growth deletion and discovery of a novel fusion transcript between suppressor of cytokine signaling-2 (Socs-2) and viral encoded semaphorin receptor (Plexin C1).
The high growth (HG) mouse mutation is a 460 Kb deletion of chromosome 10 which causes a 30-50% increase in growth in the homozygous animal. We have shotgun sequenced six bacterial artificial chromosomes which span the length of the deletion to an average depth of 13.2x to generate a 649,868 bp sequence. Sequence analysis revealed the presence of three genes, suppressor of cytokine signaling-2 (Socs-2), caspase and RIP adaptor with death domain (Raidd/Cradd), and viral encoded semaphorin receptor (Plexin C1, viral encoded semaphorin receptor). The two deletion breakpoints lie in within the second introns of both Socs-2 and Plexin C1, resulting in the formation of a novel expressed fusion transcript between Socs-2 and Plexin C1 in HG mice. Expression of the fusion transcript, the presence of four splice variants of Raidd/Cradd and the exon structure of Socs-2 were illustrated using polymerase chain reaction. Genomic comparisons of the mouse and human sequence were used to verify the sequence assembly.
Wong
Marisa L
ML
Department of Animal Science, University of California, One Shields Avenue, Davis, CA 95616-8521, USA.
Islas-Trejo
Alma
A
Medrano
Juan F
JF
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001483:Base Sequence; D053420:CRADD Signaling Adaptor Protein; D002352:Carrier Proteins / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000235:genetics*; D019457:Chromosome Breakage / Q000235:genetics; D002872:Chromosome Deletion; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins; D005091:Exons; D015870:Gene Expression; D005796:Genes / Q000235:genetics; D006801:Humans; D007438:Introns; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D012097:Repressor Proteins; D017422:Sequence Analysis, DNA; D050826:Suppressor of Cytokine Signaling Proteins; D015534:Trans-Activators
D048868:Adaptor Proteins, Signal Transducing; D053420:CRADD Signaling Adaptor Protein; C104252:CRADD protein, human; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; C484481:Cradd protein, mouse; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D012097:Repressor Proteins; C106643:SOCS2 protein, human; C491409:Socs2 protein, mouse; D050826:Suppressor of Cytokine Signaling Proteins; D015534:Trans-Activators; C091529:plexin; D004247:DNA