Incorporation of DNA and protein precursors into macromolecules by bacteria at -15 degrees C.
DNA and protein precursors were incorporated into trichloroacetic acid-precipitated material by bacterial cell suspensions during incubation for 50 to 100 days at -15 degrees C. Incorporation did not occur at -70 degrees C and was inhibited by antibiotics. The results demonstrate that bacteria can perform macromolecular synthesis under conditions that mimic entrapment in glacial ice.
Christner
Brent C
BC
Department of Microbiology, The Ohio State University, Columbus 43210-1292, USA. bchristner@montana.edu
D001419:Bacteria / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D004269:DNA, Bacterial / Q000096:biosynthesis*; D005615:Freezing; D007930:Leucine / Q000378:metabolism; D046911:Macromolecular Substances; D013936:Thymidine / Q000378:metabolism
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D046911:Macromolecular Substances; D007930:Leucine; D013936:Thymidine