title: Differences in virulence factors among clinical isolates of Escherichia coli causing cystitis and pyelonephritis in women and prostatitis in men.
abstract: Differences in the presence of nine urovirulence factors among clinical isolates of Escherichia coli causing cystitis and pyelonephritis in women and prostatitis in men have been studied. Hemolysin and necrotizing factor type 1 occur significantly more frequently among isolates causing prostatitis than among those causing cystitis (P < 0.0001) or pyelonephritis (P < 0.005). Moreover, the papGIII gene occurred more frequently in E. coli isolates associated with prostatitis (27%) than in those associated with pyelonephritis (9%) (P < 0.05). Genes encoding aerobactin and PapC occurred significantly less frequently in isolates causing cystitis than in those causing prostatitis (P < 0.01 and P < 0.0001, respectively) and pyelonephritis (P < 0.01 and P < 0.0001, respectively). No differences in the presence of Sat or type 1 fimbriae were found. Finally, AAFII and Bfp fimbriae are no longer considered uropathogenic virulence factors since they were not found in any of the strains analyzed. Overall, the results showed that clinical isolates producing prostatitis need greater virulence than isolates producing pyelonephritis in women or, in particular, cystitis in women (P < 0.05). Overall, the results suggest that clinical isolates producing prostatitis are more virulent that those producing pyelonephritis or cystitis in women.
authors.affiliation: Servei de Microbiologia, Institut Clínic Infecciones i Inmunología, Hospital Clínic-IDIBAPS, Barcelona, Spain. joruiz@clinic.ub.es
authors.forename: Joaquim
authors.initials: J
authors.lastname: Ruiz
authors.forename: Karine
authors.initials: K
authors.lastname: Simon
authors.forename: Juan P
authors.initials: JP
authors.lastname: Horcajada
authors.forename: Maria
authors.initials: M
authors.lastname: Velasco
authors.forename: Margarita
authors.initials: M
authors.lastname: Barranco
authors.forename: Gloria
authors.initials: G
authors.lastname: Roig
authors.forename: Antonio
authors.initials: A
authors.lastname: Moreno-Martínez
authors.forename: Jose A
authors.initials: JA
authors.lastname: Martínez
authors.forename: Teresa
authors.initials: T
authors.lastname: Jiménez de Anta
authors.forename: Josep
authors.initials: J
authors.lastname: Mensa
authors.forename: Jordi
authors.initials: J
authors.lastname: Vila
mesh_terms: D003556:Cystitis / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D010861:Fimbriae, Bacterial / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D008297:Male; D011472:Prostatitis / Q000382:microbiology*; D011704:Pyelonephritis / Q000382:microbiology*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D037521:Virulence Factors