title: Morphological alterations induced by cold-shock in Neurospora crassa.
abstract: The effects of cold-shock on Neurospora crassa was here investigated. In cold-shock treated hyphae (6 h at 4 degrees C) the branching pattern was changed and dichotomy as well as a fan-like-pattern structure were temporarily detected at the tips. When exogenous Ca(2+) (500 mM) was added to the hyphae, these morphological alterations were not detected. In addition, the protein electrophoretic profile of cultures submitted to cold-shock was different from that of cultures kept at 30 degrees C. After 1 and 2 hours at 4 degrees C at least 5 bands were more intense than at 30 degrees C. The results suggest that cold-shock temporarily increases the synthesis of some proteins probably involved in the acclimatization process at 4 degrees C, and that the effects on morphology are probably due to damage to calcium influx.
authors.affiliation: Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café s/n, CEP 14040-903--Ribeirão Preto--SP--Brasil.
authors.forename: Cristina Yoshiko
authors.initials: CY
authors.lastname: Kawano
authors.forename: Suraia
authors.initials: S
authors.lastname: Said
mesh_terms: D002118:Calcium / Q000378:metabolism; D003080:Cold Temperature; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis; D025301:Hyphae / Q000166:cytology / Q000254:growth & development; D025282:Mycelium / Q000302:isolation & purification; D009492:Neurospora crassa / Q000737:chemistry / Q000166:cytology* / Q000254:growth & development; D013997:Time Factors
chemical_list: D005656:Fungal Proteins; D002118:Calcium