Glycosaminoglycan-mimetic biomaterials. 3. Glycopolymers prepared from alkene-derivatized mono- and disaccharide-based glycomonomers.
Mono- and disaccharide-containing glycopolymers were synthesized by two different free-radical processes, and their ability to act as heparan sulfate glycomimetics in promoting the binding of Fibroblast Growth Factor-2 (FGF-2) to its receptor (FGFR-1) was evaluated using an in vitro cell-based assay. Cyanoxyl (*OC triple bond N)-mediated polymerization of acrylamide with alkene-derivatized mono- and disaccharides including sulfated or nonsulfated N-acetyl-D-glucosamine is described. The results of this approach are compared to those obtained via the classical ammonium peroxodisulfate (APS)/N,N,N',N'-tetramethylethylenediamine (TMEDA) initiating system and confirm the capacity of cyanoxyl-mediated polymerization to generate a variety of glycopolymers with high saccharide contents and low polydispersity indexes. In vitro assays demonstrate that specific glycopolymers can potentiate FGF-2/FGFR-1 binding interactions.
Baskaran
Subramanian
S
Laboratory for Biomolecular Materials Research, Department of Surgery and Biomedical Engineering, Emory University, Atlanta, Georgia 30322, USA.
Grande
Daniel
D
Sun
Xue-Long
XL
Yayon
Avner
A
Chaikof
Elliot L
EL
D000475:Alkenes / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D016466:CHO Cells; D002236:Carbohydrate Conformation; D006224:Cricetinae; D004187:Disaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry*; D015394:Molecular Structure; D009005:Monosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D011485:Protein Binding; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D051496:Receptor, Fibroblast Growth Factor, Type 1; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism
D000475:Alkenes; D001672:Biocompatible Materials; D004187:Disaccharides; D005609:Free Radicals; D006025:Glycosaminoglycans; D009005:Monosaccharides; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1