The construction and characterization of an amperometric immunosensor for the thyroid hormone (+)-3,3',5,5'-tetraiodo-L-thyronine (L-T4).
A novel amperometric immunosensor, based on graphite paste (graphite powder and paraffin oil), has been constructed for the assay of L-T4. The graphite paste is impregnated with mouse monoclonal anti-(+)-3,3',5,5'-tetraiodo-L-thyronine (anti-L-T4). The immunosensor can be reliably used for the assay of L-T4 in thyroid and in drugs, using chronoamperometry technique, at ppt up to ppb concentration levels. The potential used for L-T4 assay was +450 mV vs. Ag/AgCl electrode. The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new assay.
Stefan
Raluca-Loana
RL
Department of Chemistry, University of Pretoria, South Africa. raluca.stefan@chem.up.ac.za
Aboul-Enein
Hassan Y
HY
D002624:Chemistry, Clinical / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007120:Immunochemistry; D008956:Models, Chemical; D013963:Thyroid Hormones / Q000032:analysis*; D013974:Thyroxine / Q000032:analysis* / Q000097:blood
D013963:Thyroid Hormones; D013974:Thyroxine