Optimization of a fermentation medium for the production of Penicillin G acylase from Bacillus sp.
OBJECTIVE
Optimization of Penicillin G acylase (PAC) production from a novel isolate of Bacillus sp.


METHODS
Fermentation medium for PAC production was optimized using a two-level fractional factorial design with seven components.


RESULTS
A maximum production of 9.5 U ml(-1) of PAC was obtained in an optimized medium containing (g l(-1): K2HPO4, 1.0; MgSO4.7H2O, 0.1; CaCl2.2H2O, 0.1; PAA, 2.0; tryptone, 5.0; yeast extract, 3.0; and sucrose, 50.0.


CONCLUSIONS
The two-step medium optimization resulted in a twofold increase in PAC production. Since the strain Bacillus sp. PGS10 produces a high level of PAC, it could be a potential candidate for industrial production of PAC.
Rajendhran
J
J
Department of Microbial Technology, Centre for Advanced Studies in Functional Genomics, School of Biological Sciences, Madurai Kamaraj University, Madurai, India.
Krishnakumar
V
V
Gunasekaran
P
P
D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development*; D001431:Bacteriological Techniques; D018533:Biomass; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods; D010399:Penicillin Amidase / Q000096:biosynthesis*; D012988:Soil Microbiology
D003470:Culture Media; D010399:Penicillin Amidase