title: Hypoxia activates multiple transcriptional pathways in mouse pheochromocytoma cells.
abstract: Mouse pheochromocytoma cells (MPCs) provide an excellent model system for investigating the effects of hypoxia on catecholamine enzyme genes and on transcription factors mediating stress responses. RT-PCR detects rapid, transient increases in PNMT mRNA in hypoxic MPC 712 cells. Additionally, elevation of mRNAs encoding transcription factors hypoxia inducible factor 1 (HIF-1) alpha subunit and Egr-1 are evident within 60 min incubation in anoxia. Therefore, hypoxia elicits rapid transcriptional responses in numerous genes expressed by chromaffin cells.
authors.affiliation: Department of Pediatrics, State University of New York at Stony Brook, Stony Brook, New York 11794, USA. mevinger@notes.cc.sunysb.edu
authors.forename: Marian J
authors.initials: MJ
authors.lastname: Evinger
authors.forename: Stefan
authors.initials: S
authors.lastname: Cikos
authors.forename: Victoria
authors.initials: V
authors.lastname: Nwafor-Anene
authors.forename: James F
authors.initials: JF
authors.lastname: Powers
authors.forename: Arthur S
authors.initials: AS
authors.lastname: Tischler
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000818:Animals; D002395:Catecholamines / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D000860:Hypoxia; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism; D010673:Pheochromocytoma / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014157:Transcription Factors; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D002395:Catecholamines; D004268:DNA-Binding Proteins; C497443:Hif1a protein, mouse; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors