Longevity of neutralizing antibody levels in macaques vaccinated with Quil A-adjuvanted measles vaccine candidates.
Quil A-based candidate measles vaccines have been shown to be immunogenic and protective in cotton rats and macaques. Here we studied the longevity of protective VN antibody levels induced in macaques with one dose of measles virus (MV) iscom. Inactivated MV adjuvanted with iscom-matrix or with purified Quillaja saponin QA-22 were also tested. All animals developed high levels of VN antibody and MV-specific IFNgamma-producing cells. Especially the high VN antibody levels induced by the latter two preparations showed virtually no decrease during the 2-year follow-up. These highly promising candidate MV vaccines should now be tested in infant macaques in the presence or absence of passively transferred and/or maternally derived VN antibodies. In addition, the immunopathological safety of the constructs should be evaluated in the atypical measles model in rhesus macaques.
Stittelaar
Koert J
KJ
Institute of Virology, Erasmus MC, PO Box 1738, 3000 DR, Rotterdam, The Netherlands. stittelaar@viro.fgg.eur.nl
Vos
Helma W
HW
van Amerongen
Geert
G
Kersten
Gideon F A
GF
Osterhaus
Albert D M E
AD
de Swart
Rik L
RL
D000276:Adjuvants, Immunologic / Q000737:chemistry; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology* / Q000502:physiology; D008213:Lymphocyte Activation; D008457:Measles / Q000276:immunology / Q000517:prevention & control; D008458:Measles Vaccine / Q000276:immunology*; D008459:Measles virus / Q000276:immunology; D062605:Quillaja Saponins; D012503:Saponins / Q000737:chemistry*; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014616:Vaccinia virus; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D008458:Measles Vaccine; D062605:Quillaja Saponins; D012503:Saponins; D014614:Vaccines, Synthetic; D014760:Viral Fusion Proteins; C046386:Quil A