title: Preliminary crystallographic studies of the creatinine amidohydrolase from Pseudomonas putida.
abstract: Creatinine amidohydrolase (creatininase; EC 3.5.2.10) from Pseudomonas putida has been overexpressed in Escherichia coli and crystallized by the hanging-drop method. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 102.0, b = 150.7, c = 167.1 A. Native data were collected to 1.8 A resolution by a rotation method at 100 K using an ADSC Quantum 4R CCD detector with synchrotron radiation.
authors.affiliation: Biotechnology, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ito
authors.forename: Naota
authors.initials: N
authors.lastname: Kanada
authors.forename: Takahiko
authors.initials: T
authors.lastname: Inoue
authors.forename: Kumiko
authors.initials: K
authors.lastname: Furukawa
authors.forename: Kinuyo
authors.initials: K
authors.lastname: Yamashita
authors.forename: Nobutada
authors.initials: N
authors.lastname: Tanaka
authors.forename: Kazuo T
authors.initials: KT
authors.lastname: Nakamura
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nishiya
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sogabe
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshimoto
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D011487:Protein Conformation; D016958:Pseudomonas putida / Q000201:enzymology*
chemical_list: D000581:Amidohydrolases; C019946:creatininase