title: Intra-nuclear RNA trafficking: insights from live cell imaging.
abstract: Despite recent advances, the mechanisms of RNA movements and targeting within the nucleus are still mysterious. While diffusion appears to play a crucial role in nuclear dynamics and RNA transport, some data argue for a model in which diffusion is controlled, at least in part, by the organization of the nucleus in well-defined compartments. Much of the recent progress is based on imaging technologies, and this review will first present them in some detail. We will then summarize studies that analyzed nuclear movements of both polyadenylated RNA and box C/D snoRNP. Indeed, this latter model has already brought a number of interesting results. We will finally present some of our original results on box C/D snoRNA transport.
authors.affiliation: IGMM-CNRS UMR 5535, IFR24, 1919, route de Mende, 34293 Montpellier cedex 5, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Boulon
authors.forename: Eugenia
authors.initials: E
authors.lastname: Basyuk
authors.forename: Jean Marie
authors.initials: JM
authors.lastname: Blanchard
authors.forename: Edouard
authors.initials: E
authors.lastname: Bertrand
authors.forename: Céline
authors.initials: C
authors.lastname: Verheggen
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000378:metabolism; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D038761:Photobleaching; D012313:RNA / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012313:RNA