title: Isolation and identification of novel ADP-ribosylated proteins from Streptomyces coelicolor A3(2).
abstract: An important role of protein ADP-ribosylation in bacterial morphogenesis has been proposed (J. Bacteriol. 178, 3785-3790; 178, 4935-4941). To clarify the detail of ADP-ribosylation, we identified a new kind of target protein for ADP-ribosylation in Streptomyces coelicolor A3(2) grown to the late growth phase. All four proteins (MalE, BldKB, a periplasmic protein for binding branched-chain amino-acids, and a periplasmic solute binding protein) were functionally similar and participated in the regulation of transport of metabolites or nutrients through the membrane. ADP-ribosylation was likely to occur on a cysteine residue, because the modification group was removed by mercuric chloride treatment. The modification site may be the site of lipoprotein modification necessary for protein export. This report is the first suggesting that certain proteins involved in membrane transport can be ADP-ribosylated.
authors.affiliation: National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Sugawara
authors.forename: Naoshi
authors.initials: N
authors.lastname: Dohmae
authors.forename: Koji
authors.initials: K
authors.lastname: Kasai
authors.forename: Hisako
authors.initials: H
authors.lastname: Saido-Sakanaka
authors.forename: Susumu
authors.initials: S
authors.lastname: Okamoto
authors.forename: Koji
authors.initials: K
authors.lastname: Takio
authors.forename: Kozo
authors.initials: K
authors.lastname: Ochi
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification; D008627:Mercuric Chloride / Q000494:pharmacology; D008969:Molecular Sequence Data; D013302:Streptomyces / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D006023:Glycoproteins; D000246:Adenosine Diphosphate Ribose; D008627:Mercuric Chloride