title: Generation of a sequence characterized amplified region probe for authentication of crocodilian species.
abstract: A 209-base pair (bp) crocodilian-specific sequence characterized amplified region (SCAR) was identified from a 425-bp randomly amplified polymorphic DNA (RAPD) fragment. The 209-bp SCAR was produced from amplifications of DNA extracted from fresh and/or dry meat samples from at least three species of Crocodylus, Caiman crocodylus, and Alligator mississippiensis. No amplification was observed from DNA of other common animal species. The use of SCAR opens the way for quick authentication of crocodilian samples for conservation biology and trade regulation.
authors.affiliation: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
authors.forename: Forrest Chung-Fai
authors.initials: FC
authors.lastname: Yau
authors.forename: Ka-Lok
authors.initials: KL
authors.lastname: Wong
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Paul Pui-Hay
authors.initials: PP
authors.lastname: But
authors.forename: Pang-Chui
authors.initials: PC
authors.lastname: Shaw
mesh_terms: D000489:Alligators and Crocodiles / Q000145:classification* / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003132:Commerce; D015342:DNA Probes / Q000235:genetics*; D004463:Ecology; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D015342:DNA Probes; D012336:RNA, Ribosomal, 16S