title: PrP genotyping of Austrian sheep breeds.
abstract: Scrapie, an ovine and caprine transmissible spongiforme encephalopathy, is widely spread among sheep populations in many European countries. As it is known that susceptibility to scrapie is determined genetically, breeding programmes aiming at providing scrapie-resistant flocks have been established. Selection is based on the prion protein (PrP) genotype, which is used to classify animals into risk groups of susceptibility (R1-R5) according to the amino acids encoded by codons at positions 136, 154 and 171, respectively. At position 136 (136V-->136A) alanine and at position 154 (154H-->154R) as well as 171 (171Q-->171R) arginine are the favoured amino acids. Whereas PrP genotyping data are available for many of the European sheep breeds, comparable data for local Austrian sheep breeds are missing. The most known among these are Tyrolean mountain sheep, forest sheep. Tyrolean stone sheep and Carynthian sheep. The genotypes of 112 sheep from these four local breeds were determined. In terms of PrP genetics, Austrian breeds belong to the group of non-valine-breeds, with the exception of the Carynthian sheep, that exhibited a frequency of 136V of 4.2%. The most frequent allele was ARQ with 64.6-71.2% (depending on the breed), followed by ARR (14.8-25.8%). In contrast to the above-mentioned findings, scrapie has never been diagnosed in any of the Austrian sheep breeds. Native Austrian sheep breeds exhibit a very robust constitution, a pronounced adaptation to harsh climates and good reproduction parameters as well as a marked mother instinct. Therefore, these breeds are often used in crossbreeding programmes. Beside the above-mentioned characteristics, our results indicate that the investigated breeds may be effectively used in crossing-out breeding programmes for eliminating valine at position 136 of PrP.
authors.affiliation: II Medical Clinic for Ruminants and Swine, University of Veterinary Medicine, Vienna, Austria. wolfgang.sipos@vu-wien.ac.at
authors.forename: W
authors.initials: W
authors.lastname: Sipos
authors.forename: M
authors.initials: M
authors.lastname: Kraus
authors.forename: F
authors.initials: F
authors.lastname: Schmoll
authors.forename: R
authors.initials: R
authors.lastname: Achmann
authors.forename: W
authors.initials: W
authors.lastname: Baumgartner
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D001317:Austria; D001947:Breeding; D017931:DNA Primers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011328:Prions / Q000235:genetics*; D011897:Random Allocation; D012608:Scrapie / Q000235:genetics*; D012756:Sheep / Q000235:genetics
chemical_list: D017931:DNA Primers; D011328:Prions