Determining the genetic structure of the natural population of Staphylococcus aureus: a comparison of multilocus sequence typing with pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing.
We used a sample of Staphylococcus aureus strains that are carried by humans and that are representative of the natural population of S. aureus strains in order to assess the value of multilocus sequence typing (MLST), pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing as epidemiological tools. Only MLST was able to define clonal complexes unambiguously. All DNA-based typing approaches achieved a high degree of agreement, implying phylogenetic concordance, but predicted epidemiological associations with variable accuracy.
Grundmann
Hajo
H
Division of Microbiology and Infectious Diseases, University Hospital Nottingham, Nottingham NG7 2UH, United Kingdom. Hajo.Grundmann@Nottingham.ac.uk
Hori
Satoshi
S
Enright
Mark C
MC
Webster
Carol
C
Tami
Adriana
A
Feil
Edward J
EJ
Pitt
Tyrone
T
D015373:Bacterial Typing Techniques; D001434:Bacteriophage Typing; D004269:DNA, Bacterial / Q000032:analysis*; D017753:Ecosystem; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D009666:Nose / Q000382:microbiology*; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D013204:Staphylococcus Phages / Q000145:classification; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics*
D004269:DNA, Bacterial