Modifying subsceral fluid pressure in an experimental model of mitomycin-C diffusion.
BACKGROUND
Episcleral application of mitomycin-C (MMC) during glaucoma filtration surgery hinders the post-operative wound healing. Diffusion through the sclera might result in a toxic effect on the ciliary body resulting in reduced aqueous humor production leading to post-operative hypotony. We developed an experimental model to investigate the influence of intraocular pressure on the diffusion of MMC through the sclera and in subscleral compartments.


METHODS
Scleral quadrants of 10 human donor eyes were mounted on PMMA tubes filled with saline imitating the intraocular volume. By height variation of a coupled infusion line different intraocular pressures were simulated (0, 8, 23 and 80 mmHg). Additionally the model included a subscleral sponge to mimic the compartment of the ciliary body. The episcleral sides of the scleral quadrants were exposed for 1 min to sponges soaked with 200 microg ml(-1) MMC. An 8-mm-diameter scleral disk was punched out with a trephine and horizontally dissected with a kryotome. The MMC concentrations of scleral layers, epi-and subscleral sponges and the fluid within the tubes were analysed by means of high-performance liquid chromatography.


RESULTS
The MMC concentration gradually declined from the episcleral sponge (165 microg ml(-1)) to the superficial (3.3 microg ml(-1)) and deep scleral layers (1.2 microg ml(-1)), and to the subscleral sponge (0.2 microg ml(-1)). We were able to detect very small concentrations of MMC in the fluid within the PMMA tubes (0.01 microg ml(-1)).


CONCLUSIONS
We developed a new experimental in vitro model for investigating transscleral MMC diffusion. The different simulated intraocular pressures had no effect on the concentration gradient through the investigated compartments of our model.
Georgopoulos
M
M
Department of Ophthalmology, Medical School, University of Vienna, Währinger Gürtel 18-20, Vienna A-1090, Austria. michael.georgopoulos@akh-wien.ac.at
Vass
C
C
Vatanparast
Z
Z
D004058:Diffusion; D006801:Humans; D066298:In Vitro Techniques; D007429:Intraocular Pressure; D007430:Intraoperative Care; D016685:Mitomycin / Q000493:pharmacokinetics*; D008954:Models, Biological; D019384:Nucleic Acid Synthesis Inhibitors / Q000493:pharmacokinetics*; D012590:Sclera / Q000378:metabolism*; D014130:Trabeculectomy
D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin