[Inhibition of alpha-interferon and cinnamic acid on proliferation of human lung cancer cell].
OBJECTIVE
Recently, the research showed that cinnamic acid (CINN) could inhibit some cancer cells. This study was designed to investigate the effects of alpha-interferon and CINN on proliferation of human lung cancer cells.


METHODS
Human lung adenocarcinoma cell A549 were treated with alpha-interferon and CINN. The growth curve, soft-agar colony-forming rate, and methyl greenpyronine staining were used to analyze the inhibition to cell proliferation. The cell cycle was detected by flow cytometry(FCM).


RESULTS
alpha-interferon and CINN could markedly inhibit proliferation of A549 (P < 0.05), promote the differentiation (P < 0.05), and reduce the colony-forming rate in the soft-agar drastically(P < 0.05), FCM showed that the cell cycle was arrested in the G1/G0 phase.


CONCLUSIONS
alpha-interferon and CINN can inhibit the proliferation of lung adenocarcinoma cell. Combination of the alpha-interferon and CINN show the stronger effect that the alpha-interferon or CINN alone.
Jin
Ge
G
Department of Biochemistry, Medical College, Zhengzhou University, Zhengzhou 450052, P. R. China. xinxin@371.net
Zhang
Ting
T
Wang
Tao
T
Yang
Li-ping
LP
D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002934:Cinnamates / Q000494:pharmacology*; D004357:Drug Synergism; D005434:Flow Cytometry; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology; D013997:Time Factors; D014407:Tumor Cells, Cultured
D002934:Cinnamates; D016898:Interferon-alpha; C029010:cinnamic acid