[The relationship between tumor necrosis factor A-308 gene polymorphism and susceptibility of severe periodontitis in adults].
OBJECTIVE
To investigate the relationship between tumor necrosis factor A-308 gene polymorphism and susceptibility of severe periodontitis in adults.


METHODS
Buccal swabs from 65 adult patients with severe periodontitis and 96 periodontal healthy adult controls were obtained. DNA was extracted from each sample using Chelex-100 method, then PCR-RFLP was performed to determine the TNFA-308 genotype. Data were analyzed by statistics software.


RESULTS
A significant difference of genotype distribution was found between the patients and controls. The percentage of 1/1 type of the patients was much higher (P < 0.001). Risk Analysis showed odds ratio for allele 1 was 4.64.


CONCLUSIONS
This study suggested that TNFA-308 allele 1 maybe a marker of susceptibility to severe periodontitis.
Qian
Wei
W
Department of Periodontics, Beijing Stomatology Hospital, Capital University of Medical Sciences, Beijing 100050, China.
Zhang
Jincai
J
Zhang
Yunhui
Y
D000328:Adult; D020022:Genetic Predisposition to Disease; D015804:HLA-DR4 Antigen / Q000235:genetics; D006801:Humans; D010518:Periodontitis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D015804:HLA-DR4 Antigen; D014409:Tumor Necrosis Factor-alpha