title: Angiotensin II induces prostaglandin E(2) release in human gingival fibroblasts.
abstract: We investigated the effect of angiotensin II on prostaglandin E(2) release in human gingival fibroblasts. Stimulation of human gingival fibroblasts with angiotensin II elicited prostaglandin E(2) release in a concentration- and time-dependent manner. Angiotensin III also induced prostaglandin E(2) release, but the effect was weaker than that of angiotensin II. Angiotensin II- and angiotensin III-induced prostaglandin E(2) release was inhibited by AT(1) receptor antagonist FR-130,739, but not AT(2) receptor antagonist PD-123,319. Angiotensin II evoked an increase in intracellular Ca(2+) in fura-2-loaded human gingival fibroblasts. These results suggest that angiotensin II functions as a physiological mediator via Ca(2+)-mobilizing AT(1) receptor activation in human gingival fibroblasts.
authors.affiliation: Department of Physiology, Nihon University School of Dentistry at Matsudo, Chiba 271-8587, Japan.
authors.forename: Masaomi
authors.initials: M
authors.lastname: Segawa
authors.forename: Sumi
authors.initials: S
authors.lastname: Nakao
authors.forename: Yorimasa
authors.initials: Y
authors.lastname: Ogata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiya
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Furuyama
mesh_terms: D000804:Angiotensin II / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000805:Angiotensin III / Q000494:pharmacology; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D014662:Vasoconstrictor Agents / Q000494:pharmacology*
chemical_list: D014662:Vasoconstrictor Agents; D000804:Angiotensin II; D000805:Angiotensin III; D015232:Dinoprostone; D002118:Calcium