Tissue specific, sex and age--related differences in the 6-phosphogluconate dehydrogenase gene expression.
Data presented in thid paper indicate that: (1) the age-related changes in 6-phosphogluconate dehydrogenase (6PGDH) activity depend on sex and tissue. No differences in the liver 6PGDH activity between young (1-month-old) males and females were found. In adult males, the activity was the same as in young animals but, in adult females, it reached the value twice as high as in the young. In adipose tissue (both white and brown) and kidney cortex, the enzyme activity decreased with age both in males and females. There were no differences between males and females 6PGDH activity in brain, heart and skeletal muscle. (2) The sex and age-related changes in the liver 6PGDH activity occur predominantly at the level of mRNA cellular concentration. (3) In the liver of ovariectomized rats decrease of 6PGDH activity and mRNA level was observed. Oestradiol administration to ovariectomized rats restored liver 6PGDH activity and liver 6PGDH mRNA levels to that observed in controls. No changes in 6PGDH activity and mRNA levels were found in white adipose tissue (WAT) of ovariectomized adult rats and in ovariectomized rats treated with oestradiol. (4) Oestradiol administration to males caused an increase of liver 6PGDH activity and mRNA levels to values observed in females, but was without an effect on WAT 6PGDH activity and mRNA level. (5) These results suggest that 6PGDH activity in different tissues is not regulated in coordinate fashion and that oestradiol plays an important role in the liver enzyme activity regulation.
Pankiewicz
Areta
A
Department of Biochemistry, Medical University of Gdansk, 80-211, Gdansk, Poland.
Sledzinski
Tomasz
T
Nogalska
Anna
A
Swierczynski
Julian
J
D000273:Adipose Tissue / Q000201:enzymology / Q000378:metabolism; D000367:Age Factors; D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D004958:Estradiol / Q000097:blood / Q000494:pharmacology; D005227:Fatty Acids / Q000096:biosynthesis; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009928:Organ Specificity; D010052:Ovariectomy / Q000009:adverse effects; D010734:Phosphogluconate Dehydrogenase / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012737:Sex Factors
D005227:Fatty Acids; D012333:RNA, Messenger; D004958:Estradiol; D010734:Phosphogluconate Dehydrogenase