title: Enhancement of oxidative response and cytokine production by yam mucopolysaccharide in murine peritoneal macrophage.
abstract: Yam (Dioscorea batatas) mucopolysaccharide (YMP) was examined for the ability to induce secretory and cellular responses in murine peritoneal macrophages. When macrophages were treated with various doses (10-100 microg/ml) of YMP for 48 h, a significant cytotoxicity against B 16 cells was observed. YMP also increased the myeloperoxidase activity and the production of H(2)O(2), O(2)(-), nitric oxide (NO) and tumor necrosis factor (TNF-alpha). However, there was a little alteration on the production of IFN-gamma. These results indicate that reactive oxygen intermediates (ROI), reactive nitrogen intermediates (RNI) and TNF-alpha are likely major mediators of cytotoxic activity of YMP-treated macrophages.
authors.affiliation: Department of Biotechnology & Bioproducts Research Center, Yonsei University, Seoul, South Korea.
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Choi
authors.forename: Jae-Kwan
authors.initials: JK
authors.lastname: Hwang
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis*; D027742:Dioscorea / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006025:Glycosaminoglycans / Q000737:chemistry / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D010100:Oxygen / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D016207:Cytokines; D006025:Glycosaminoglycans; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D009195:Peroxidase; D010100:Oxygen