Polyribosome loading of spinach mRNAs for photosystem I subunits is controlled by photosynthetic electron transport.
In light-, but not in dark-grown spinach seedlings, the mRNAs for the nuclear-encoded photosystem I subunits D, F and L are associated with polyribosomes and this association is prevented by the application of 3-(3',4'-dichlorophenyl)-1,1'-dimethyl urea (DCMU), an inhibitor of the photosynthetic electron transport. To identify the cis-elements which are responsible for this regulation, we generated a series of chimeric PsaD constructs and tested them in transgenic tobacco. The spinach PsaD 5'-untranslated region is sufficient to confer light- and photosynthesis-dependent polyribosome association onto the uidA reporter gene, while the tobacco PsaD 5'-untranslated region directs constitutive polyribosome association. These results are discussed with regard to signals from photosynthetic electron flow which control processes in the cytoplasm.
Sherameti
Irena
I
Institut für Allgemeine Botanik, Lehrstuhl Pflanzenphysiologie, Friedrich-Schiller-Universität Jena, Dornburger Str. 159, Germany.
Nakamura
Masayaki
M
Yamamoto
Yoshiharu Y
YY
Pfannschmidt
Thomas
T
Obokata
Junichi
J
Oelmüller
Ralf
R
D001483:Base Sequence; D003624:Darkness; D004579:Electron Transport; D008027:Light; D008969:Molecular Sequence Data; D010788:Photosynthesis; D045331:Photosystem I Protein Complex / Q000235:genetics*; D030821:Plants, Genetically Modified; D011132:Polyribosomes / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D018724:Spinacia oleracea / Q000235:genetics* / Q000378:metabolism*; D013997:Time Factors; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism
D045331:Photosystem I Protein Complex; D012333:RNA, Messenger