title: Functional recovery after facial nerve crush is delayed in severe combined immunodeficient mice.
abstract: The goal of the current study was to determine if T and B lymphocytes play a role in functional recovery after peripheral nerve injury. The time course of behavioral recovery following facial nerve crush injury at the stylomastoid foramen was established in scid mice which lack functional T and B cells and reconstituted scid mice as compared to wild-type mice. The average time necessary for recovery of full eye blink reflex and vibrissae movements in wild-type mice was 10.3+/-0.2 and 9.9+/-0.34 days, respectively. In contrast, recovery of full eye blink reflex and vibrissae movements took 14.8+/-0.54 and 12.3+/-0.41 days, respectively, in scid mice. Reconstitution of scid mice with whole splenocytes resulted in functional recovery times similar to wild-type, with eye blink reflex recovery and vibrissae movement being 10.5+/-0.3 and 10.0+/-0.0 days, respectively. These results suggest that the delayed behavioral recovery time observed in scid mice may be due to the absence of T and B lymphocytes.
authors.affiliation: Department of Cell Biology, Neurobiology and Anatomy, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USA. cserpe@luc.edu
authors.forename: Craig J
authors.initials: CJ
authors.lastname: Serpe
authors.forename: Julie E
authors.initials: JE
authors.lastname: Tetzlaff
authors.forename: Susanna
authors.initials: S
authors.lastname: Coers
authors.forename: Virginia M
authors.initials: VM
authors.lastname: Sanders
authors.forename: Kathryn J
authors.initials: KJ
authors.lastname: Jones
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001522:Behavior, Animal; D002470:Cell Survival / Q000276:immunology; D020220:Facial Nerve Injuries / Q000276:immunology* / Q000473:pathology; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D016513:Mice, SCID; D009046:Motor Neurons / Q000166:cytology / Q000276:immunology*; D009409:Nerve Crush; D009416:Nerve Regeneration / Q000276:immunology; D015213:Neuroimmunomodulation / Q000502:physiology; D020127:Recovery of Function / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology