Macrophages and dendritic cells use the cytosolic pathway to rapidly cross-present antigen from live, vaccinia-infected cells.
Professional APCs (pAPC) can process and present on their own MHC class I molecules Ags acquired from Ag donor cells (ADC). This phenomenon of cross-presentation is essential in the induction of CD8(+) T cell responses to viruses that do not infect pAPC and possibly contributes to the induction of CD8(+) responses to many other viruses. However, little is known about the mechanisms underlying this process. In this study, we show that dendritic cells and macrophages cross-present a model Ag supplied by vaccinia virus-infected ADC via the cytosolic route. Strikingly, we also found that cross-presentation of Ags provided by vaccinia-infected cells occurs within a couple of hours of pAPC/ADC interaction, that the duration of cross-presentation lasts for only 16 h, and that cross-presentation can occur at early times of infection when the ADC are still alive.
Ramirez
Maria Carmen
MC
Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
Sigal
Luis J
LJ
D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism / Q000821:virology; D000941:Antigens / Q000378:metabolism; D016923:Cell Death / Q000276:immunology; D018920:Coculture Techniques; D003600:Cytosol / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D006825:Hybridomas; D007739:L Cells; D008264:Macrophages / Q000166:cytology / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism; D015398:Signal Transduction / Q000276:immunology*; D013997:Time Factors; D014616:Vaccinia virus / Q000276:immunology*
D000941:Antigens; D010446:Peptide Fragments; D010047:Ovalbumin