Regulation of APC-Cdc20 by the spindle checkpoint.
The spindle checkpoint ensures the fidelity of chromosome segregation in mitosis and meiosis. In response to defects in the mitotic apparatus, it blocks the activity of the anaphase-promoting complex, a large ubiquitin ligase required for chromosome segregation. Recent studies indicate that the spindle checkpoint monitors both the attachment of chromosomes to the mitotic spindle and the tension across the sister chromatid generated by microtubules. Upon checkpoint activation, checkpoint protein complexes containing BubR1(Mad3), Bub3, Mad2 and Cdc20 directly bind to the anaphase-promoting complex and inhibit its ligase activity. Therefore, the checkpoint proteins form a complex intracellular signalling network to inhibit the anaphase-promoting complex.
Yu
Hongtao
H
Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9041, USA. hongtao.yu@utsouthwestern.edu
D000705:Anaphase; D064173:Anaphase-Promoting Complex-Cyclosome; D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D002352:Carrier Proteins; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins / Q000378:metabolism*; D020090:Chromosome Segregation; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D008025:Ligases / Q000378:metabolism*; D046911:Macromolecular Substances; D064246:Mad2 Proteins; D008540:Meiosis; D008870:Microtubules / Q000502:physiology; D008938:Mitosis; D008957:Models, Genetic; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D008941:Spindle Apparatus / Q000502:physiology*; D043743:Ubiquitin-Protein Ligase Complexes
C577495:CDC20 protein, S cerevisiae; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; C577695:MAD2 protein, S cerevisiae; D046911:Macromolecular Substances; D064246:Mad2 Proteins; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D008025:Ligases