Measurement of bisphenol A levels in human blood serum and ascitic fluid by HPLC using a fluorescent labeling reagent.
A sensitive column-switching HPLC method with fluorescence detection was developed for the determination of bisphenol A (BPA) in human blood serum and ascitic fluid samples. 4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) was used as the fluorescent label, and the excess reagent was removed by a column-switching technique. Liquid-liquid extraction with chloroform was used for the pretreatment of serum and ascitic fluid samples. BPA in both the samples could be determined in the concentration range of 0.1-7.0 ppb with the detection limit of 0.04 ppb at a signal-to-noise ratio of 3. The recoveries of BPA spiked to serum and ascitic fluid were 78.6 and 77.7%, respectively. The mean concentrations of BPA (n=9) in maternal and umbilical cord blood sera obtained from healthy pregnant women were 0.46+/-0.20 and 0.62+/-0.13 ppb, respectively. BPA levels (n=21) in blood sera and ascitic fluid obtained from the patients with sterility were also determined to be 0.46+/-0.20 and 0.56+/-0.19 ppb, respectively. Relationships of BPA concentrations were observed between maternal and umbilical cord blood serum samples (r=0.626), as well as blood serum and ascitic fluid samples (r=0.785).
Kuroda
Naotaka
N
Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan. n-kuro@net.nagasaki-u.sc.jp
Kinoshita
Yumi
Y
Sun
Yen
Y
Wada
Mitsuhiro
M
Kishikawa
Naoya
N
Nakashima
Kenichiro
K
Makino
Tsunehisa
T
Nakazawa
Hiroyuki
H
D001202:Ascitic Fluid / Q000737:chemistry*; D001559:Benzhydryl Compounds; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D005456:Fluorescent Dyes / Q000032:analysis*; D006801:Humans; D010636:Phenols / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D011247:Pregnancy; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D005456:Fluorescent Dyes; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds