Detection and characterization of membrane-bound selenoproteins in the kidney of the rat.
The essential trace element selenium is of fundamental importance to human biology. Information on the membrane-bound selenoproteins was obtained by labeling of rats in vivo with (75)Se-selenite, subcellular fractionation of the tissue homogenates, separation of the proteins by SDS-PAGE, two-dimensional electrophoresis, and selenium detection by autoradiography. In this way, in addition to already known membrane-bound selenoproteins three novel selenoproteins in the molecular mass range of 38-40, 18, and 12-13 kDa were detected in the microsomal membranes.
Kyriakopoulos
A
A
Department of Molecular Trace Element Research in the Life Sciences, Hahn-Meitner-Institut, D-14109 Berlin, Germany. kyriakopoulos@hmi.de
Bertelsmann
H
H
Graebert
A
A
Hoppe
B
B
Kuhbacher
M
M
Behne
D
D
D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D007668:Kidney / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000172:deficiency*; D012644:Selenium Radioisotopes; D051140:Selenoproteins
D000596:Amino Acids; D011506:Proteins; D012644:Selenium Radioisotopes; D051140:Selenoproteins; D012643:Selenium