title: Sulfated polysaccharides from red microalgae have antiinflammatory properties in vitro and in vivo.
abstract: The primary goal of the present research was to determine whether sulfated polysaccharides derived from red microalgae possess antiinflammatory properties when directed against specific parameters of human skin inflammation. These unique biopolymers were studied in both in vitro and in vivo models of skin inflammation. Human subjects were recruited to participate in a study in which the polysaccharide material was applied topically and shown to inhibit cutaneous erythema induced by a known irritant. Leukocyte migration from capillary blood into sites of inflammation is an essential component of the inflammatory process and occurs in a series of steps, two of which are adhesion and chemotaxis. In vitro, the polysaccharide material primarily inhibited the migration of polymorphonuclear leukocytes (PMNs) toward a standard chemoattractant molecule and also partially blocked adhesion of PMNs to endothelial cells. The data obtained strongly suggest that sulfated polysaccharides derived from red microalgae have significant beneficial potential for use in topical products. In addition, the data suggested that the antiinflammatory mechanism for the polysaccharide was, at least in part, due to inhibition of circulating immune cell recruitment toward inflammatory stimuli.
authors.affiliation: Biological Research Division, The Estee Lauder Companies, 125 PineLawn Road, Melville, NY 11747, USA. mmatsui@estee.com
authors.forename: Mary S
authors.initials: MS
authors.lastname: Matsui
authors.forename: Neelam
authors.initials: N
authors.lastname: Muizzuddin
authors.forename: Shoshana
authors.initials: S
authors.lastname: Arad
authors.forename: Kenneth
authors.initials: K
authors.lastname: Marenus
mesh_terms: D000287:Administration, Topical; D000328:Adult; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001453:Balsams; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004727:Endothelium / Q000378:metabolism; D004890:Erythema / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D000461:Rhodophyta / Q000737:chemistry; D012867:Skin / Q000187:drug effects*; D012882:Skin Tests
chemical_list: D000893:Anti-Inflammatory Agents; D001453:Balsams; D011134:Polysaccharides; C025080:Peruvian balsam