A new 68Ge/68Ga generator system using an organic polymer containing N-methylglucamine groups as adsorbent for 68Ge.
A macroporous styrene-divinylbenzene copolymer containing N-methylglucamine groups was selected for a new 68Ge/68Ga generator system. This resin packed into a column effectively adsorbed the parent nuclide 68Ge. The daughter 68Ga was eluted from the resin with a solution of a low-affinity gallium chelating ligand such as citric or phosphoric acid. The 68Ge leakage was less than 0.0004% of the 68Ge adsorbed on the resin. By simple mixing of transferrin and desferoxamine conjugated HSA and IgG with the eluate from the column, 68Ga-labeling was completed in high yield.
Nakayama
M
M
Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-Machi, Nagasaki, 852-8131, Japan. morio@net.nagasaki-u.ac.jp
Haratake
M
M
Ono
M
M
Koiso
T
T
Harada
K
K
Nakayama
H
H
Yahara
S
S
Ohmomo
Y
Y
Arano
Y
Y
D000327:Adsorption; D001798:Blood Proteins / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods; D004588:Electrophoresis, Cellulose Acetate; D005710:Gallium Radioisotopes / Q000737:chemistry*; D005720:Gamma Rays; D005857:Germanium / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007553:Isotope Labeling; D008536:Meglumine / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011108:Polymers / Q000737:chemistry*; D011876:Radionuclide Generators; D019275:Radiopharmaceuticals / Q000737:chemistry
D001798:Blood Proteins; D005710:Gallium Radioisotopes; D011108:Polymers; D019275:Radiopharmaceuticals; D005857:Germanium; D008536:Meglumine