[Effect of inhibitor RNA on intracellular inhibition of viral gene expression in 5'-noncoding region of hepatitis C virus].
OBJECTIVE
To determine the effect of internal ribosome entry site (IRES)specific inhibitor RNA (IRNA) on viral gene expression mediated by HCV 5' noncoding region (NCR) in human hepatic carcinoma cell line.


METHODS
Cotransfection of pCMVNCRluc containing 5'NCR-luc fusion genes and eukaryotic vectors of IRNA into human hepatic carcinoma cells (HHCC) was performed and the luciferase activity of reporter gene was measured by luminometer.


RESULTS
pCMVNCRluc was efficiently expressed in HHCC. IRES specific IRNA significantly inhibited the expression of luc mediated by HCV IRES by 50% to 90%, but the mutant IRNA lost the activity of inhibition.


CONCLUSIONS
IRNA can inhibit HCV IRES mediated gene expression in vivo.
Liang
Xuesong
X
Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. ghx69514@163.com
Zhou
Yongxing
Y
Lian
Jianqi
J
Nie
Qinghe
Q
Jia
Zhansheng
Z
D020121:5' Untranslated Regions / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects*; D005814:Genes, Viral; D015316:Genetic Therapy; D016174:Hepacivirus / Q000187:drug effects / Q000235:genetics*; D006526:Hepatitis C / Q000628:therapy; D006801:Humans; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D020121:5' Untranslated Regions; D012330:RNA, Double-Stranded