title: Aspects of nucleosomal positional flexibility and fluidity.
abstract: Nucleosomes have been considered until recently to be stable and uniquely localized particles. We focus here on two properties of nucleosomes that are emerging as central attributes of their functions: mobility and multiplicity of localization. The biological relevance of these phenomena is based on the fact that chromatin functions depend on the relative stability of nucleosomes, on their covalent or conformational modifications, their dynamics, their localization, and the density of their distribution. In order to understand these complex behaviors both the structure of the nucleosome core particles and the informational rules governing their interaction with defined DNA sequences are here taken into consideration. The fact that nucleosomes solve the problem of how to locate a specific interaction site on a potentially infinite combination of sequences, with interactions recurring to a controlled level of informational ambiguity and stochasticity, is discussed. Nucleosomes have been shown to slide along DNA. This novel facet of their behavior and its implications in chromatin remodeling are reviewed.
authors.affiliation: Centro Acidi Nucleici, CNR c/o Dipartimento di Genetica e Biologia Molecolare, Università di Roma La Sapienza, Rome, Italy.
authors.forename: Micaela
authors.initials: M
authors.lastname: Caserta
authors.forename: Loredana
authors.initials: L
authors.lastname: Verdone
authors.forename: Ernesto
authors.initials: E
authors.lastname: Di Mauro
mesh_terms: D004247:DNA / Q000378:metabolism; D009068:Movement; D009707:Nucleosomes / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D009707:Nucleosomes; D004247:DNA