Relationship between biomarker activity and developmental endpoints in Chironomus riparius Meigen exposed to an organophosphate insecticide.
The biomarkers acetylcholinesterase (AChE) and glutathione S-transferase (GST) were measured in fourth-instar Chironomus riparius Meigen larvae exposed to the organophosphate insecticide pirimiphos methyl (0, 5, 10, and 50ng/g) for 48 or 96h, and at high or low food ration. Larvae exposed to 50ng/g pirimiphos methyl died within 48h. The weight of larvae exposed to 10ng/g pirimiphos methyl was significantly lower than those exposed to 0 and 5ng/g. AChE activity was significantly reduced in larvae exposed to 10ng/g, but GST activity remained unaffected. Lower food ration reduced larval weights across all treatments but did not affect biomarker measurements. Insecticide exposure was associated with a longer time to adult emergence and oviposition, fewer egg masses, a greater proportion of deformed egg masses, and fewer eggs.
Crane
Mark
M
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, UK. craneconsultants@aol.com
Sildanchandra
Wanwisu
W
Kheir
Rania
R
Callaghan
Amanda
A
D000110:Acetylcholinesterase / Q000032:analysis*; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002683:Chironomidae / Q000254:growth & development*; D004305:Dose-Response Relationship, Drug; D004531:Eggs; D023381:Endpoint Determination; D005260:Female; D005508:Food Deprivation; D005982:Glutathione Transferase / Q000032:analysis*; D007306:Insecticides / Q000009:adverse effects*; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D009946:Organothiophosphorus Compounds / Q000009:adverse effects*
D015415:Biomarkers; D007306:Insecticides; D009946:Organothiophosphorus Compounds; C014153:pirimiphos methyl; D005982:Glutathione Transferase; D000110:Acetylcholinesterase