title: [Antitumoral effect of HSV-tk suicide gene associated with ganciclovir in an experimental model of head and neck epidermoid carcinoma].
abstract: BACKGROUND
  We studied the transfection by adenoviral vectors and the antitumoral effect of HSV-tk gene associated with ganciclovir (AdCMVtk/GCV) in KB human oral cavity squamous cell carcinoma, in vitro and in vivo.
  
  
  METHODS
  Transfection was assessed by the X-gal stain. It was used in cell cultures and tumoral sections previously exposed to adenoviral vector AdCMVlacZ. In vitro, in order to study the antitumoral effect of AdCMVtk/GCV, survival of cell cultures exposed to AdCMVtk/GCV and to AdCMVlacZ/GCV was compared. In vivo, necrotic volume as a percentage of total tumoral volume, was compared between AdCMVtk/GCV treated group and AdCMVlacZ/GCV exposed group. Hepatic and renal toxicities were assessed.
  
  
  RESULTS
  In vitro, survival of cell cultures treated with AdCMVtk/GCV was less than AdCMVlacZ/GCV exposed cells. In vivo, necrotic volume was larger in AdCMVtk/GCV treated group than in AdCMVlacZ/GCV exposed group. No toxicity was found (hepatic, renal).
  
  
  CONCLUSIONS
  KB cells are transfected by adenoviral vectors and are killed by AdCMVtk/GCV, both in vitro and in vivo (no toxicity was found in the animal model).
authors.affiliation: Dept. de Otorrinolaringología, Clínica Universitaria de Navarra, Pío XII, 36, 31008 Pamplona. boleasag@teleline.es
authors.forename: M S
authors.initials: MS
authors.lastname: Boleas Aguirre
authors.forename: S
authors.initials: S
authors.lastname: Fernández González
authors.forename: M A
authors.initials: MA
authors.lastname: Gallego Madrid
authors.forename: R
authors.initials: R
authors.lastname: García-Tapia Urrutia
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000628:therapy*; D003627:Data Interpretation, Statistical; D004195:Disease Models, Animal; D015774:Ganciclovir / Q000494:pharmacology; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D009062:Mouth Neoplasms / Q000628:therapy*; D018139:Simplexvirus / Q000235:genetics; D013997:Time Factors; D014162:Transfection; D014170:Transformation, Genetic; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D015774:Ganciclovir