An indigenously developed method for sweat collection and estimation of chloride for diagnosis of cystic fibrosis.
An indigenously developed method for sweat collection and titration method for estimation of chloride was validated. The mean difference in estimated chloride value from the known strength of saline in 50 samples was -1.04 +/- 4.13 mEq/L (95% CI: -0.07 to 2.28). The mean difference in the estimated chloride values between two observers when the test was performed on known strengths of saline solution was -2.5 +/- 4.24 mEq/L (95% CI: -3.67 to 1.33). The inter observer variability between two observers when the test was performed on sweat samples obtained from 50 individuals was -1.12 +/- 4.34 mEq/L (95% CI: -2.23 to 0.8 ). Sweat weight of more than 100 mg could be collected in first attempt in 602 of 757 (80%) patient with an average sweat weight of 230 mg. This inexpensive method of sweat collection and chloride estimation has acceptable accuracy and repeatability and can be used in resource poor setting for making a diagnosis of cystic fibrosis.
Kabra
S K
SK
Department of Pediatrics, All India Institute of Medical Sciences, New Delhi 110 029, India. skkabra@hotmail.com
Kabra
M
M
Gera
S
S
Lodha
R
R
Sreedevi
K N
KN
Chacko
S
S
Mathew
J
J
Shastri
S
S
Ghosh
M
M
D003550:Cystic Fibrosis / Q000175:diagnosis*; D007478:Iontophoresis; D015588:Observer Variation; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods*; D013542:Sweat / Q000737:chemistry*