[Effect of boanmycin on apoptosis and cell cycle of human esophageal cancer(Eca-109) cells].
OBJECTIVE
The anticancer mechanism of boanmycin was thought to inhibit the synthesis of protein in human esophageal cancer cell line. But it is not clear that there are other mechanisms of boanmycin to produce its anticancer effect on human esophageal cancer. This study was designed to examine the effect of boanmycin on apoptosis and cell cycle of human esophageal cancer cells (Eca-109).


METHODS
Microscopic observation, flow cytometry, and agarose gel electrophoresis were used to determine the induction of apoptosis and the changes of cell cycle.


RESULTS
The human esophageal cancer xenografts in nude mice treated by boanmycin showed morphological changes of different apoptotic phases. In vitro, boanmycin (50-125 micrograms/ml, 30-36 hours) elicited typical apoptotic changes in Eca-109 cells and typical DNA ladder of apoptotic cells appeared. The Eca-109 cells treated by boanmycin for 42 hours were blocked at G2/M phase.


CONCLUSIONS
Boanmycin could induce apoptosis of Eca-109 cells and block the cell cycle at G2/M phase.
Tang
Hao
H
Department of Internal Medicine, Hubei Cancer Hospital, Wuhan 430079, P. R. China.
Yang
Xiao-ping
XP
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001761:Bleomycin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D004938:Esophageal Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000648:ultrastructure; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
D004273:DNA, Neoplasm; C052656:bleomycin A6; D001761:Bleomycin