Inhibitory effects of Choto-san (Diao-teng-san), and hooks and stems of Uncaria sinensis on free radical-induced lysis of rat red blood cells.
The present study is designed to test our hypothesis that the ingestion of Uncaria sinensis (US), the main medicinal plant of Choto-san (Diao-teng-san, CS), would protect red blood cell (RBC) membrane from free radical-induced oxidation if polyphenolics in US could be absorbed and circulated in blood. When incubated with RBC suspension, Choto-san extract (CSE) and Uncaria sinensis extract (USE) exhibited strong protection for RBC membrane against hemolysis induced by 2,2-azo-bis (2-amidinopropane) dihydrochloride (AAPH), an azo free-radical initiator. The inhibitory effect was dose-dependent at concentrations of 50 to 1000 microg/mL. Ingestion of 200 mg of USE was associated with a significant decrease in susceptibility of RBC to hemolysis in rats. Furthermore, caffeic acid, an antioxidative hydroxycinnamic acid, was identified in rat plasma after administration of URE.
Sekiya
N
N
Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Toyama, Japan. s114@ms.toyama-mpu.ac.jp
Shimada
Y
Y
Shibahara
N
N
Takagi
S
S
Yokoyama
K
K
Kasahara
Y
Y
Sakakibara
I
I
Terasawa
K
K
D000578:Amidines / Q000494:pharmacology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal; D004912:Erythrocytes / Q000187:drug effects*; D005609:Free Radicals / Q000009:adverse effects; D006461:Hemolysis / Q000187:drug effects*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D032070:Uncaria
D000578:Amidines; D004365:Drugs, Chinese Herbal; D005609:Free Radicals; D010936:Plant Extracts; C120250:choto-san; C046728:2,2'-azobis(2-amidinopropane)