title: [The effect of cryopreservation on human sperm chromosome].
abstract: OBJECTIVE
  To evaluate the effects of cryopreservation on human sperm chromosome.
  
  
  METHODS
  Sperm chromosome were acquired using in vitro fertilization of zona-free hamster oocytes and human sperm. The frequency of sperm chromosome anomalies and sex chromosomes ratio before and after freezing and with different freezing methods were compared.
  
  
  RESULTS
  There were no significant differences of frequency of sperm chromosome anomalies among fresh, fast frozen and slow frozen sperm (9.40%, 7.48% and 8.74%) (P > 0.75) or ratios of sex chromosomes (P > 0.90).
  
  
  CONCLUSIONS
  These studies indicate that cryopreservation does not exert effects on human sperm chromosome.
authors.affiliation: Department of Urology, First Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Pu
authors.forename: Zai-Xian
authors.initials: ZX
authors.lastname: Chen
mesh_terms: D002875:Chromosomes; D015925:Cryopreservation; D006801:Humans; D008297:Male; D013094:Spermatozoa / Q000502:physiology*