Identification of two genes differentially expressed upon different spatial configuration of the MGH-U3 human bladder cancer cells.
By using mRNA differential display technology, we have compared gene expression in neoconfluent (actively proliferating) and 14-day confluent (non-proliferative) MGH-U3 cells. Two clones (6.17.6 and 10.9.2) displayed significantly increased expression in the 14-day confluent MGH-U3 cells, as confirmed by Northern blot analysis. Nucleotide sequence showed that clone 6.17.6 was part of the human insulin-like growth factor binding protein-5 (IGFBP-5) cDNA, while clone 10.9.2 was part of a human gene with unknown function (clone HSI12602). The differential IGFBP-5 expression in this model system was also evidenced by Western analysis. The possible anti-proliferative role of IGFBP-5 in this model system is briefly discussed.
Champetier
Serge
S
Centre Hospitalier Universitaire de Québec, Centre de recherche du Pavillon l'Hôtel-Dieu de Québec, 9 rue McMahon, Québec City, Québec, Canada G1R 2J6.
Fradet
Yves
Y
Bachvarov
Dimcho
D
D001483:Base Sequence; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018975:Insulin-Like Growth Factor Binding Protein 5 / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology*
D018975:Insulin-Like Growth Factor Binding Protein 5; D012333:RNA, Messenger; D012334:RNA, Neoplasm