title: [Allelic analysis on chromosome 5 in sporadic colorectal cancer patients].
abstract: OBJECTIVE
  To evaluate and map the putative tumor suppressor loci on chromosome 5 involved in tumor progress or metastasis.
  
  
  METHODS
  Chromosome 5 of 83 patients with sporadic colorectal cancer was systemically screened. Fifteen microsatellite marker primers labeled with 3 different fluorescents were used to amplify the corresponding loci of the genome DNA. The PCR products were electrophoresed on a 377 PRISM sequencer and the fluorescent signals were analyzed with Genotyper and Genescan software.
  
  
  RESULTS
  The highest loss of heterozygosity (LOH) ratio was found at D5S416 (48.15%) on 5p and at D5S471 (38.71%) on 5q. The region (5q13.3 - 31), where D5S471 and 3 neighboring loci (D5S428, D5S2027 and D5S2115) reside, presented high frequent LOH.
  
  
  CONCLUSIONS
  The deletion of APC, MCC, CTNNA1 and IL cluster in the 5q 13.3 - 31.1 area play important role in the tumorogenesis of colorectal cancer, and the expected existence of another novel tumor suppressor gene on 5p is possible.
authors.affiliation: Department of General Surgery, First People's Hospital, Shanghai 200080, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.forename: Chongzhi
authors.initials: C
authors.lastname: Zhou
authors.forename: Yun
authors.initials: Y
authors.lastname: Ling
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Qiu
authors.forename: Shaochun
authors.initials: S
authors.lastname: Bai
authors.forename: Wanqing
authors.initials: W
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.forename: Zhihai
authors.initials: Z
authors.lastname: Peng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D002872:Chromosome Deletion; D002895:Chromosomes, Human, Pair 5; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged