A retrospective look at the cationic peanut peroxidase structure.
The cationic peanut peroxidase has been studied in detail, not only with regard to its peptide structure, but also to the sites and role of the three moieties linked to it. Peanut peroxidase lends itself well to a close examination as a potential example for other plant peroxidase studies. It was the first plant peroxidase for which a 3-D structure was derived from crystals, with the glycans intact. Subsequent analysis of peroxidases structures from other plants have not shown great differences to that of the peanut peroxidase. As the period of proteomics follows on the era of genomics, the study of glycans has been brought back into focus. With the potential use of peroxidase as a polymerization agent for industry, there are some aspects of the overall structure that should be kept in mind for successful use of this enzyme. A variety of techniques are now available to assay for these structures/moieties and their roles. Peanut peroxidase data are reviewed in that light, as well as defining some true terms for isozymes. Because a high return of the enzyme in a pure form has been obtained from cultured cells in suspension culture, a brief review of this is also offered.
van Huystee
Robert B
RB
Department of Plant Sciences, The University of Western Ontario, London, Ontario, N6A 5B7, Canada. huystee@uwo.ca
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D010367:Arachis / Q000201:enzymology*; D002118:Calcium / Q000737:chemistry; D002412:Cations; D006031:Glycosylation; D006418:Heme / Q000737:chemistry; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D010544:Peroxidases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011134:Polysaccharides / Q000737:chemistry
D002412:Cations; D007527:Isoenzymes; D010940:Plant Proteins; D011134:Polysaccharides; D006418:Heme; D010544:Peroxidases; D002118:Calcium