Introduction of a Na+/H+ antiporter gene from Atriplex gmelini confers salt tolerance to rice.
We engineered a salt-sensitive rice cultivar (Oryza sativa cv. Kinuhikari) to express a vacuolar-type Na+/H+ antiporter gene from a halophytic plant, Atriplex gmelini (AgNHX1). The activity of the vacuolar-type Na+/H+ antiporter in the transgenic rice plants was eight-fold higher than that in wild-type rice plants. Salt tolerance assays followed by non-stress treatments showed that the transgenic plants overexpressing AgNHX1 could survive under conditions of 300 mM NaCl for 3 days while the wild-type rice plants could not. These results indicate that overexpression of the Na+/H+ antiporter gene in rice plants significantly improves their salt tolerance.
Ohta
Masaru
M
Plantech Research Institute, 1000 Kamoshida-cho, Aoba-ku, Yokohama, 227-0033, Kanagawa, Japan.
Hayashi
Yasuyuki
Y
Nakashima
Asae
A
Hamada
Akira
A
Tanaka
Akira
A
Nakamura
Tatsunosuke
T
Hayakawa
Takahiko
T
D029748:Caryophyllaceae / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D012313:RNA / Q000378:metabolism; D012492:Salts / Q000494:pharmacology*; D012964:Sodium / Q000494:pharmacology; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics* / Q000502:physiology*; D013050:Spectrometry, Fluorescence; D013997:Time Factors; D014170:Transformation, Genetic
D012492:Salts; D017923:Sodium-Hydrogen Exchangers; D012313:RNA; D012964:Sodium