Identification, cloning, and sequencing of different cytokine genes in four species of owl monkey.
Non-human primates could prove to be suitable models for the study of infectious diseases such as malaria, tuberculosis, and hepatitis; the molecules of their immune systems are in the process of being fully characterized. Due to the relevance of cytokines in the modulation of the immune response, a molecular analysis of these proteins in non-human primates from the Aotus genus was carried out. Peripheral blood mononuclear cells from four species of Aotusmonkey were obtained and their mRNAs for interleukin-2 (IL-2), IL-4, IL-6, IL-10, interferon-gamma (IFN), and tumor necrosis factor (TNF)-alpha were characterized. This study shows a high degree of conservation between nucleotide and amino acid sequences of cytokines from different Aotus species and those from humans. The TNF-alpha molecules were identical in amino acid sequences for both.
Hernández
Edith C
EC
Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
Suárez
Carlos F
CF
Méndez
Jairo A
JA
Echeverry
Sandra J
SJ
Murillo
Luis A
LA
Patarroyo
Manuel E
ME
D000595:Amino Acid Sequence; D000818:Animals; D016645:Aotidae / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D016207:Cytokines / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D016207:Cytokines; D012333:RNA, Messenger