title: High initial activity but low storage stability observed for several preparations of subtilisin Carslberg suspended in organic solvents.
abstract: Colyophilization with methyl-beta-cyclodextrin activates subtilisin Carlsberg by more than 200-fold in organic solvents, though this is a short-lived effect. About 93% of the enzyme's high initial activity observed in THF (at 45 degrees C) decreases exponentially with a t(1/2) of 1.8 h, until it reaches a residual activity (of 7%) that remains constant throughout the 4 days duration of the experiment. A further study of this enzyme reveals a general trend: the initial activities of the lyophilized powder and the cross-linked enzyme crystals are also greatly reduced upon incubation in this solvent, although these preparations retain 50% of their activity after about 20 h of incubation. All of the preparations studied retained some residual activity (which persisted throughout the duration of the experiments) after the initial exponential decay. The data here presented suggest that the mode of enzyme preparation is an important issue to consider when planning lengthy reactions.
authors.affiliation: Department of Chemistry, University of Puerto Rico at Humacao, Humacao, Puerto Rico 00791.
authors.forename: Susimar González
authors.initials: SG
authors.lastname: Martínez
authors.forename: Edgardo
authors.initials: E
authors.lastname: Alvira
authors.forename: Liz Vergara
authors.initials: LV
authors.lastname: Cordero
authors.forename: Amaris
authors.initials: A
authors.lastname: Ferrer
authors.forename: Ileana
authors.initials: I
authors.lastname: Montañés-Clemente
authors.forename: Gabriel
authors.initials: G
authors.lastname: Barletta
mesh_terms: D004795:Enzyme Stability; D005612:Freeze Drying; D006207:Half-Life; D011309:Preservation, Biological; D012997:Solvents; D020860:Subtilisin / Q000737:chemistry*; D013997:Time Factors
chemical_list: D012997:Solvents; D020860:Subtilisin