Effect of fixation period on HER2/neu gene amplification detected by fluorescence in situ hybridization in invasive breast carcinoma.
This study investigated if formalin fixation duration affects HER2/neu gene amplification detection by fluorescence in situ hybridization (FISH) in breast cancer. Tumor tissues from 35 cases were divided into three groups and subjected to two formalin fixation protocols per group (12 hr, 27 hr in the first; 2 hr, 17.5 hr in the second; 28.5 hr, 541 hr in the third) before FISH analysis. There was no significant difference in FISH signal detection between the two different fixation protocols in the first two groups. In the third, no signal was detected in 4/6 cases fixed for an extended duration.
Selvarajan
Sathiyamoorthy
S
Department of Anatomy, National University of Singapore, Singapore General Hospital, Singapore.
Bay
Boon-Huat
BH
Choo
Andrew
A
Chuah
Khoon-Leong
KL
Sivaswaren
Christina Rudduck
CR
Tien
Sim-Leng
SL
Wong
Chow-Yin
CY
Tan
Puay-Hoon
PH
D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005404:Fixatives; D005557:Formaldehyde; D005784:Gene Amplification; D018734:Genes, erbB-2; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009361:Neoplasm Invasiveness; D013997:Time Factors; D016707:Tissue Fixation / Q000379:methods
D005404:Fixatives; D005557:Formaldehyde