[The role of mitogen-activated protein kinase (MAPK) pathway in the action mechanism of PYM-induced KB cells apoptosis].
OBJECTIVE
To study the role of mitogen-activated protein kinase (MAPK) pathway in the action mechanism of PYM-induced KB cell apoptosis.


METHODS
Western blot analysis was used to investigate the expression of the mitogen-activated protein kinase.


RESULTS
When treated with PYM in cultured KB cells, the extracellular signal-regulated kinase (ERKl/2) showed a dose-and time-dependent decreasing in phosphorylation status of these proteins through a western blot analysis, whereas protein levels of p38 MAPK remained unchanged.


CONCLUSIONS
The mitogen-activated protein kinase (MAPK) pathway may play an important ro1e in PYM-induced apoptosis of KB cells.
Dai
Guowei
G
Department of Oral and Maxillofacial Surgery, Peking University School of Stomatolog, Beijing 100081, China.
Zhang
Zhenkang
Z
Zhou
Mingyong
M
Yu
Shifeng
S
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001761:Bleomycin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006801:Humans; D007624:KB Cells; D020928:Mitogen-Activated Protein Kinases / Q000502:physiology*; D048051:p38 Mitogen-Activated Protein Kinases
D000903:Antibiotics, Antineoplastic; D001761:Bleomycin; C021771:zhengguangmycin; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases