Preparation of dissociated zebrafish spinal neuron cultures.
The method described here explains a simple protocol for how to prepare dissociated Zebrafish spinal neuron cultures. The neurons grow fast in a simple culture medium and at room temperature. Considering the advantages afforded by the optical transparency of the Zebrafish embryo combined with the powerful molecular perturbation techniques available, this technique has potential to further advance molecular analysis of axon growth and guidance.
Andersen
S S
SS
University of California at Berkeley, Department of Molecular and Cellular Biology, 142 Life Sciences Addition #3200, Berkeley, CA 94720-3200, USA. soren@sorenandersen1.org
D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D004210:Dissection / Q000295:instrumentation / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology*; D005820:Genetic Testing / Q000639:trends; D020439:Growth Cones / Q000187:drug effects / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology*; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology*; D014357:Trypsin; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins / Q000235:genetics / Q000302:isolation & purification
D003470:Culture Media; D029961:Zebrafish Proteins; D014357:Trypsin