title: Complete cDNA sequence encoding 20S proteasome alpha 5 subunit PAE from soybean.
abstract: The 20S proteasome is the proteolytic complex that is responsible for degrading short-lived and abnormal proteins, especially those targeted by ubiquitin conjugation. The complex exists, as a hollow cylinder shaped structure comprised of four stacked rings. The outer rings contain 7 alpha subunits and the inner rings contain 7 beta subunits. In this study, we report the amino acid sequence of the alpha 5 subunit (PAE) in soybean (Gylcine max) based on the cDNA sequence. The amino acid sequence identity is 96% with the Arabidops alpha 5 subunit and 95% with the rice alpha 5 subunit. The highly conserved sequence homology suggests there is an important biological role for this proteasome.
authors.affiliation: Department of Genetic Engineering, College of Natural Sciences, University of Suwon, San 2-2 Wauri, Bongdamup Whasungsi Kyonggido, Suwon, 445-743, South Korea. pbpark@mail.suwon.ac.kr
authors.forename: Phun Bum
authors.initials: PB
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D018744:DNA, Plant; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D005810:Multigene Family / Q000235:genetics; D046988:Proteasome Endopeptidase Complex; D021122:Protein Subunits / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013025:Glycine max / Q000201:enzymology* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D009097:Multienzyme Complexes; D021122:Protein Subunits; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex