title: NORs inheritance analysis in crossings including individuals from two stocks of rainbow trout (Oncorhynchus mykiss).
abstract: Silver nitrate staining of rainbow trouts (Oncorhynchus mykiss) chromosomes, for the identification of the nucleolar organizing regions (NORs), revealed that in individuals from Núcleo Experimental de Salmonicultura de Campos do Jordão (Brazil) NORs were located in the long arms of submetacentric pair while in specimens from Mount Shasta (USA) NORs were located in the short arms of a submetacentric pair. Cytogenetic analysis of the offspring, obtained through artificial crosses including individuals from both stocks, allowed the identification of NORs in two submetacentric chromosomes, one in the short arms and the other in the long arms, confirming the effectiveness of the hybridization process. Complementary results obtained using the FISH technique with 18S and 5S rDNA probes showed that NOR-bearing chromosomes exhibited a cluster of 5S genes located in tandem with the 18S gene cluster in both stocks. The results allow us to suggest that the difference in NOR-bearing chromosomes found between the two stocks is likely to be due to pericentric inversion involving the chromosome segment where 18S and 5S rDNA genes are located. The presence of ribosomal genes in the long arms of a submetacentric chromosome is apparently a particular characteristic of the rainbow trout stock of Campos do Jordão and might be used as a chromosome marker in studies of controlled crosses in this species.
authors.affiliation: Departamento de Morfologia, Instituto de Biociências, Universidade Estadaul Paulista, campus de Botucatu, 18618-000, Botucatu, SP, Brazil. fabio@ibb.unesp.br
authors.forename: Fábio
authors.initials: F
authors.lastname: Porto-Foresti
authors.forename: Claudio
authors.initials: C
authors.lastname: Oliveira
authors.forename: Yara Aiko
authors.initials: YA
authors.lastname: Tabata
authors.forename: Marcos Guilherme
authors.initials: MG
authors.lastname: Rigolino
authors.forename: Fausto
authors.initials: F
authors.lastname: Foresti
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D017404:In Situ Hybridization, Fluorescence; D009697:Nucleolus Organizer Region; D017686:Oncorhynchus mykiss / Q000235:genetics*