title: Earlier Helicobacter pylori infection increases the risk for the N-methyl-N-nitrosourea-induced stomach carcinogenesis in Mongolian gerbils.
abstract: Helicobacter pylori (H. pylori) is now well known to be associated with stomach cancer, with infection during childhood rather than as an adult considered to be more important for carcinogenesis. To evaluate the difference in susceptibility to stomach carcinogenesis in relation to age of acquisition of H. pylori infection, we designed an experiment involving inoculation of H. pylori ATCC43504 followed by N-methyl-N-nitrosourea (MNU) treatment at different ages. Four-week-old male Mongolian gerbils (MGs) were divided into twelve groups. H. pylori was inoculated at 4, 18 and 32 weeks of age, as representatives of early, middle and late infection, respectively. Two weeks later, the animals were treated with MNU. Groups without H. pylori and/or MNU were included as controls. The incidences of adenocarcinomas at 52 weeks after the inoculation in the early (H. pylori+MNU), middle (H. pylori+MNU), and late (H. pylori+MNU) group were 60% (12/20), 18.4% (2/11), and 10% (2/20), respectively. The corresponding figures were 14.8% (4/27), 0% (0/11), and 0% (0/21) in the MNU-alone groups. A higher titer of serum IgG for H. pylori and higher gastrin level were seen in the early-infected compared to the middle and the late groups (P<0.01). The results clearly demonstrated that early acquisition of H. pylori significantly increases gastric chemical carcinogenesis with MNU, as compared to the case with later infection, possibly because of differences in host gastric mucosal factors and immunologic responses.
authors.affiliation: Division of Oncological Pathology, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya, Aichi 464-8681, Japan.
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Cao
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tsukamoto
authors.forename: Koji
authors.initials: K
authors.lastname: Nozaki
authors.forename: Harunari
authors.initials: H
authors.lastname: Tanaka
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Shimizu
authors.forename: Michio
authors.initials: M
authors.lastname: Kaminishi
authors.forename: Toshiko
authors.initials: T
authors.lastname: Kumagai
authors.forename: Masae
authors.initials: M
authors.lastname: Tatematsu
mesh_terms: D000230:Adenocarcinoma / Q000209:etiology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D005755:Gastrins / Q000097:blood; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008770:Methylnitrosourea; D012306:Risk; D013274:Stomach Neoplasms / Q000209:etiology*
chemical_list: D000907:Antibodies, Bacterial; D005755:Gastrins; D007074:Immunoglobulin G; D008770:Methylnitrosourea