title: The subdomains of the transactivation domain of the aryl hydrocarbon receptor (AhR) inhibit AhR and estrogen receptor transcriptional activity.
abstract: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) activates the aryl hydrocarbon receptor (AhR) to mediate transcriptional activity of dioxin-responsive genes. The transactivation domain (TAD) of human AhR (hAhR) has potentially distinct acidic, glutamine-rich, and proline/serine/threonine-rich subdomains. Cotransfection of exogenous hAhR into BP8 cells with isolated subdomains of hAhR TAD fused to glutathione S-transferase exhibited squelching of TCDD-dependent dioxin-response element (DRE)-driven luciferase reporter-gene activity with each subdomain. To study the potential cross talk between AhR- and estrogen receptor (ER)-mediated activities, BP8 cells were cotransfected with hAhR TAD subdomain constructs and ERalpha. The three hAhR TAD subdomains inhibited the 17beta-estradiol-induced estrogen-response element-mediated reporter-gene transactivation. Cotransfection of hAhR with the ligand-binding domain (LBD) of ERalpha also squelched TCDD-dependent DRE-driven reporter-gene activity in the presence of 17beta-estradiol. Similar results were observed in T47D cells that express functional AhR and ERalpha. These results indicate that the isolated subdomains of hAhR's TAD and LBD of ERalpha are capable of squelching ligand-dependent transactivation of either the AhR or the ER, by titrating crucial proteins from an existing common pool of cofactors.
authors.affiliation: Center for Molecular Toxicology and Carcinogenesis and Department of Veterinary Science, Pennsylvania State University, 226, Fenske Lab, University Park, PA 16802, USA.
authors.forename: Rashmeet K
authors.initials: RK
authors.lastname: Reen
authors.forename: Adam
authors.initials: A
authors.lastname: Cadwallader
authors.forename: Gary H
authors.initials: GH
authors.lastname: Perdew
mesh_terms: D000595:Amino Acid Sequence; D047628:Estrogen Receptor alpha; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter; D006801:Humans; D010446:Peptide Fragments / Q000494:pharmacology*; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics*; D011960:Receptors, Estrogen / Q000235:genetics*; D011994:Recombinant Proteins / Q000494:pharmacology; D015534:Trans-Activators; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection
chemical_list: D047628:Estrogen Receptor alpha; D010446:Peptide Fragments; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D011960:Receptors, Estrogen; D011994:Recombinant Proteins; D015534:Trans-Activators