Vesicular transport.
The minimal requirements for COP I and COP II vesicle formation from liposomes are the small GTPases Arf1 and Sar1, GTP and the coat complexes themselves. Coat-nucleating factors including specific GTP-GDP exchange factors, SNAREs and other cargo molecules are likely to determine the site of vesicle formation. Tethering mediated by long rod-like coiled-coil proteins precedes docking of vesicles with their target membrane and contributes to specific recognition of this membrane. NSF/Sec18p acts prior to membrane fusion to prime specific SNARE complexes for docking of vesicles with their targets, while the SNAREs are likely to be the minimal membrane-fusion machinery.
Barr
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IBLS, Division of Biochemistry and Molecular Biology, University of Glasgow, CRC-Beatson Laboratories, Garscube Estate, Switchback Road, Glasgow G61 1BD.
D000818:Animals; D022181:COP-Coated Vesicles / Q000378:metabolism; D005057:Eukaryotic Cells / Q000378:metabolism; D006801:Humans; D008051:Lipid Bilayers / Q000378:metabolism; D008561:Membrane Fusion / Q000502:physiology; D008565:Membrane Proteins / Q000378:metabolism; D050600:SNARE Proteins; D013572:Synaptic Vesicles / Q000378:metabolism; D022161:Transport Vesicles / Q000502:physiology*; D033921:Vesicular Transport Proteins / Q000378:metabolism
D008051:Lipid Bilayers; D008565:Membrane Proteins; D050600:SNARE Proteins; D033921:Vesicular Transport Proteins