Attenuated induction of heme oxygenase after intrathecal exposure to lysed blood in mice overexpressing superoxide dismutase.
Induction of heme oxygenase-1 (HO-1) in the spinal cord was studied in adult wildtype and transgenic mice overexpressing the antioxidant copper, zinc superoxide dismutase (CuZn SOD) 24 h after intrathecal infusion of heterologous lysed blood. Double immunolabeling techniques were used to determine the extent to which HO-1 was induced in astrocytes and microglia/macrophages. HO-1 was induced in both astrocytes and microglia/macrophages in the dorsal horns near the site of infusion of lysed blood in all mice. However, the number of HO-1 labeled cells was significantly less in the transgenic as compared to the wildtype animals. Together, these findings suggest that lysed blood preferentially induces HO-1 in glia and macrophages through the generation of oxidative stress.
Weinzierl
Martin
M
Department of Neurological Surgery, RWTH Aachen, Germany.
Mautes
A E
AE
Lin
Yong
Y
Noble
Linda J
LJ
D000818:Animals; D001769:Blood; D001798:Blood Proteins / Q000494:pharmacology*; D039481:CD11b Antigen / Q000378:metabolism; D004789:Enzyme Activation / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D051547:Heme Oxygenase-1; D007150:Immunohistochemistry; D007278:Injections, Spinal; D008565:Membrane Proteins; D051379:Mice; D008822:Mice, Transgenic; D018384:Oxidative Stress / Q000502:physiology; D011897:Random Allocation; D013116:Spinal Cord / Q000098:blood supply / Q000166:cytology / Q000201:enzymology; D013482:Superoxide Dismutase / Q000187:drug effects* / Q000235:genetics
D001798:Blood Proteins; D039481:CD11b Antigen; D005904:Glial Fibrillary Acidic Protein; D008565:Membrane Proteins; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D013482:Superoxide Dismutase