Immunosuppression in specific-pathogen-free broilers administered infectious bursal disease virus vaccines by in ovo route.
The effect of two infectious bursal disease virus (IBDV) vaccines (IBDV-immune complex [Icx] and IBDV-2512), administered in ovo, on the cell-mediated immunity of specific-pathogen-free (SPF) broilers was examined. A decrease (P < 0.05) in the T-cell mitogenic response occurred in birds vaccinated with both vaccines on days 9 and 21 post in ovo vaccination (PIOV), but an increase (P < 0.05) occurred on day 15 PIOV. The T cells from birds given the IBDV-2512 were less responsive. There were no significant differences in proportions of lymphocytes expressing CT4+CT8 and CT8+CT4- except on day 21 PIOV, where an increase (P < 0.05) in IBDV-2512-vaccinated birds and a decrease (P < 0.05) in percentage of CT4+CT8- in IBDV-Icx-vaccinated birds was observed. There was an increase (P < 0.05) in percentage of CT8+CT4- T cells on day 21 PIOV in both vaccinated groups. A decrease (P < 0.05) in B-cell percentage was observed on day 21 PIOV in birds given both vaccines. Results indicated that although humoral immunosuppression is associated with destruction of B cells (bursal atrophy), cell-mediated immunosuppression induced by these two IBDV vaccines in SPF birds was not associated with altered helper (CT4+CT8-) or cytotoxic (CT8+CT4-) subpopulations of T lymphocytes.
Corley
Michelle M
MM
102 Milbank Hall, Department of Agriculture and Environmental Sciences, Tuskegee University, Tuskegee, AL 36088, USA.
Giambrone
Joseph J
JJ
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D018175:Birnaviridae Infections / Q000276:immunology / Q000662:veterinary*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002645:Chickens; D003208:Concanavalin A; D007108:Immune Tolerance; D007243:Infectious bursal disease virus / Q000276:immunology* / Q000302:isolation & purification; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D011201:Poultry Diseases / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage*
D014765:Viral Vaccines; D003208:Concanavalin A