TA-19, a novel protein antigen of Trichosporon asahii, in summer-type hypersensitivity pneumonitis.
The most common form of hypersensitivity pneumonitis in Japan is summer-type hypersensitivity pneumonitis (SHP), which is caused by the inhalation of Trichosporon asahii or Trichosporon mucoides. To seek protein antigens relevant to the immunopathogenesis of SHP, we constructed a cDNA expression library of T. asahii, a major causative yeast species of SHP. Using the immunoscreening method, we identified and cloned a novel gene encoding a 19-kD protein, named TA-19, which proved to be specifically recognized in the bronchoalveolar lavage (BAL) fluids and sera of patients with SHP. IgG, IgA, and IgM antibodies to the recombinant TA-19 protein were significantly elevated in the sera as well as in the BAL fluids from SHP patients compared with those from non-SHP groups. This protein also induced SHP-specific proliferation of the mononuclear cells from both the peripheral blood and BAL. These results reveal that TA-19 derived from T. asahii may play a relevant role in specific cellular and humoral immune responses in patients with SHP.
Matsunaga
Yasujiro
Y
Department of Pulmonary Medicine, Faculty of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan.
Usui
Yutaka
Y
Yoshizawa
Yasuyuki
Y
D000280:Administration, Inhalation; D000328:Adult; D000368:Aged; D000542:Alveolitis, Extrinsic Allergic / Q000097:blood / Q000209:etiology* / Q000235:genetics; D000908:Antibodies, Fungal / Q000235:genetics / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D000946:Antigens, Fungal / Q000008:administration & dosage / Q000009:adverse effects* / Q000276:immunology*; D001483:Base Sequence; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000276:immunology; D005260:Female; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000276:immunology; D015723:Gene Library; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D007075:Immunoglobulin M / Q000276:immunology / Q000378:metabolism; D007564:Japan; D008218:Lymphocytosis / Q000097:blood / Q000209:etiology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D011237:Predictive Value of Tests; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012621:Seasons; D014250:Trichosporon / Q000235:genetics / Q000276:immunology*
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011994:Recombinant Proteins