title: Anion selectivity by the sodium iodide symporter.
abstract: The iodide transporter of the thyroid (NIS) has been cloned by the group of Carrasco. The NIS-mediated transport was studied by electrophysiological methods in NIS-expressing Xenopus oocytes. Using this method, the anion selectivity of NIS was different from that previously reported for thyroid cells, whereas perchlorate and perrhenate were found not transported. In this study we compared the properties of human NIS, stably transfected in COS-7 cells to those of the transport in a thyroid cell line, the FRTL5 cells, by measuring the transport directly. We measured the uptake of (125)I(-), (186)ReO(4)(-), and (99m)TcO(4)(-) and studied the effect on it of known competing anions, i.e. ClO(4)(-), SCN(-), ClO(3)(-), ReO(4)(-), and Br(-). We conclude that the properties of the NIS transporter account by themselves for the properties of the thyroid iodide transporter as described previously in thyroid slices. The order of affinity was: ClO(4)(-) > ReO(4)(-) > I(-) >/= SCN(-) > ClO(3)(-) > Br(-). NIS is also inhibited by dysidenin (as in dog thyroid).
authors.affiliation: Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire, University of Brussels, School of Medicine, Erasmus Hospital, B 1070 Brussels, Belgium. jvsande@ulb.ac.be
authors.forename: J
authors.initials: J
authors.lastname: Van Sande
authors.forename: C
authors.initials: C
authors.lastname: Massart
authors.forename: R
authors.initials: R
authors.lastname: Beauwens
authors.forename: A
authors.initials: A
authors.lastname: Schoutens
authors.forename: S
authors.initials: S
authors.lastname: Costagliola
authors.forename: J E
authors.initials: JE
authors.lastname: Dumont
authors.forename: J
authors.initials: J
authors.lastname: Wolff
mesh_terms: D000818:Animals; D000838:Anions / Q000378:metabolism* / Q000494:pharmacology; D001667:Binding, Competitive; D001692:Biological Transport; D001965:Bromides / Q000378:metabolism; D019556:COS Cells; D002460:Cell Line; D002704:Chlorates / Q000378:metabolism; D006801:Humans; D007454:Iodides / Q000378:metabolism; D007457:Iodine Radioisotopes / Q000378:metabolism; D007700:Kinetics; D010087:Oxides / Q000378:metabolism; D011868:Radioisotopes; D051381:Rats; D012211:Rhenium / Q000378:metabolism; D013670:Sodium Pertechnetate Tc 99m / Q000378:metabolism; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D013861:Thiocyanates / Q000378:metabolism; D013961:Thyroid Gland / Q000378:metabolism; D014162:Transfection; D000099209:Sodium-Iodide Symporters
chemical_list: D000838:Anions; D001965:Bromides; D002704:Chlorates; D007454:Iodides; D007457:Iodine Radioisotopes; D010087:Oxides; D011868:Radioisotopes; D012211:Rhenium; D013670:Sodium Pertechnetate Tc 99m; D027981:Symporters; D013861:Thiocyanates; D000099209:Sodium-Iodide Symporters; C015428:perrhenate