title: [Establishment and comparative genomic hybridization analysis of human esophageal carcinomas cell line EC9706].
abstract: OBJECTIVE
  To establish a long-standing cell line of esophageal squamous cell carcinoma (ESCC) in pursuit of a model for in vitro study of carcinogenesis.
  
  
  METHODS
  Small tissue blocks taken from resected specimens of esophageal cancer were cultured, and cell line EC9706 was established. The biologic properties of EC9706 were characterized. Comparative genomic hybridization(CGH) was performed on the cell line.
  
  
  RESULTS
  The growth curve of EC9706 was detected. The cell generation time was 26 hours. The plate colony forming efficiency is 91.9%, with the capacity of forming clones in soft agar. EC9706 cells show high tumorigenecity as indicated by the rapid regeneration of moderate-poor-differentiated squamous cell carcinomas after injection into nude mice. CGH analysis indicated copy number gains of 1p1, 1q2-4, 2p1, 2q1, 5p, 7p14, 7q21, 11q1, 15q2, 20q and losses of 2p2, 2q2, 3p, 4, 9p, 14, 18, Xq. High-level gain of 5p was observed.
  
  
  CONCLUSIONS
  Established cell line EC9706 can serve as a useful tool for studying the carcinogenesis of ESCC.
authors.affiliation: National Laboratory of Molecular Oncology, Cancer Institute (Hospital), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021 P. R. China. wangmr@263.net.cn
authors.forename: Yaling
authors.initials: Y
authors.lastname: Han
authors.forename: Fang
authors.initials: F
authors.lastname: Wei
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Cai
authors.forename: Baosheng
authors.initials: B
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Shuhua
authors.initials: S
authors.lastname: Xia
authors.forename: Hai
authors.initials: H
authors.lastname: Hu
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Huang
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Han
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.forename: Mingrong
authors.initials: M
authors.lastname: Wang
mesh_terms: D000368:Aged; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002455:Cell Division; D002869:Chromosome Aberrations; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured