Comparative molecular dynamics simulations of amphotericin B-cholesterol/ergosterol membrane channels.
Amphotericin B (AmB) is a very effective anti-fungal polyene macrolide antibiotic whose usage is limited by its toxicity. Lack of a complete understanding of AmB's molecular mechanism has impeded attempts to design less toxic AmB derivatives. The antibiotic is known to interact with sterols present in the cell membrane to form ion channels that disrupt membrane function. The slightly higher affinity of AmB toward ergosterol (dominant sterol in fungal cells) than cholesterol (mammalian sterol) is regarded as the most essential factor on which antifungal chemotherapy is based. To study these differences at the molecular level, two realistic model membrane channels containing molecules of AmB, sterol (cholesterol or ergosterol), phospholipid, and water were studied by molecular dynamics (MD) simulations. Comparative analysis of the simulation data revealed that the sterol type has noticeable effect on the properties of AmB membrane channels. In addition to having a larger size, the AmB channel in the ergosterol-containing membrane has a more pronounced pattern of intermolecular hydrogen bonds. The interaction between the antibiotic and ergosterol is more specific than between the antibiotic and cholesterol. These observed differences suggest that the channel in the ergosterol-containing membrane is more stable and, due to its larger size, would have a higher ion conductance. These observations are in agreement with experiments.
Baginski
Maciej
M
Department of Pharmaceutical Technology and Biochemistry, Chemical Faculty, Technical University of Gdansk, Narutowicza St. 11, 80-952 Gdansk, Poland. maciekb@hypnos.chem.pg.gda.pl
Resat
Haluk
H
Borowski
Edward
E
D000666:Amphotericin B / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry*; D004875:Ergosterol / Q000737:chemistry*; D006860:Hydrogen Bonding; D015394:Molecular Structure
D000666:Amphotericin B; D002784:Cholesterol; D004875:Ergosterol