Detection and quantification of nitric oxide (NO) synthase-independent generation of NO.
Nitric oxide formation in ischemia-reperfusion injury can be identified and measured directly using EPR of nitroso-heme complexes comprising NO bound to either Mb or Hb-Fe2+. This article described the successful use of this method to detect and quantify the generation of NO formed independently of nitric oxide synthase in ischemia-reperfusion injury to skeletal muscle. The quantification of nitroso-heme complexes using EPR is recommended in ischemia-reperfusion studies of either skeletal or cardiac muscle that aim to characterize the role of nitric oxide.
Lepore
Diana A
DA
Pituitary Research Unit, Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia 3052.
D004578:Electron Spin Resonance Spectroscopy; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D009211:Myoglobin / Q000737:chemistry; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000378:metabolism*; D013113:Spin Labels
D006454:Hemoglobins; D009211:Myoglobin; D013113:Spin Labels; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase