title: Dyes for caries detection: influence on composite and compomer microleakage.
abstract: The aim of this study was to evaluate the influence of caries-detecting dyes on the microleakage of adhesive materials. Sixty cubic class V cavities were prepared on buccal and lingual surfaces of 30 human third molars. Coronal margins were located in enamel and gingival margins in cementum. The teeth were randomly divided into six groups of ten restorations each. Cavities were restored with an adhesive system (Single Bond, 3M ESPE, St. Paul, Minn., USA), a compomer (F2000, 3M ESPE), or a composite resin (Z100, 3M ESPE) according to the manufacturer's directions. Acid red dye (Seek, Ultradent, South Jordan, Ut., USA) and basic fuchsin dye (Vide Cárie, Inodon, Porto Alegre, Brazil) were tested. Control groups were prepared without the use of dyes. After 7 days of storage in distilled water, the restorations were polished and the teeth were subjected to thermal cycling followed by immersion in 2% methylene blue. The teeth were sectioned, and microleakage scores were evaluated under magnification (40x). Data were submitted to statistical analysis using the nonparametric Kruskal-Wallis test. A statistically significant difference ( P<0.05) in microleakage was found between the materials in cementum (Z100>F2000) but not in enamel. Control and experimental groups using dyes showed similar results. It was concluded that dyes for caries detection did not increase microleakage of the adhesive materials tested.
authors.affiliation: Federal University of Pelotas, School of Dentistry, Department of Operative Dentistry, Rua Gonçalves Chaves, 457 - Centro, Pelotas-RS, Brazil 96015-560. pivadent@ufpel.tche.br
authors.forename: Evandro
authors.initials: E
authors.lastname: Piva
authors.forename: Luciene
authors.initials: L
authors.lastname: Meinhardt
authors.forename: Flávio F
authors.initials: FF
authors.lastname: Demarco
authors.forename: John M
authors.initials: JM
authors.lastname: Powers
mesh_terms: D017438:Bisphenol A-Glycidyl Methacrylate; D004396:Coloring Agents / Q000009:adverse effects*; D020770:Compomers; D003188:Composite Resins; D001840:Dental Bonding; D003731:Dental Caries / Q000175:diagnosis; D003763:Dental Leakage / Q000209:etiology*; D018772:Dental Marginal Adaptation; D003793:Dental Restoration, Permanent / Q000379:methods*; D005899:Glass Ionomer Cements; D006801:Humans; D008963:Molar; D011897:Random Allocation; D012235:Rhodamines / Q000009:adverse effects; D012394:Rosaniline Dyes / Q000009:adverse effects; D012822:Silicon Dioxide; D015040:Zirconium
chemical_list: D004396:Coloring Agents; D020770:Compomers; D003188:Composite Resins; C118368:F2000 cement; D005899:Glass Ionomer Cements; D012235:Rhodamines; D012394:Rosaniline Dyes; C083568:Z100 composite resin; C115612:single bond; C022027:lissamine rhodamine B; D017438:Bisphenol A-Glycidyl Methacrylate; D012822:Silicon Dioxide; D015040:Zirconium