[Proliferation and denucleation of K562 cells induced by cytochalasin B].
OBJECTIVE
To observe the effect of cytochalasin B on the denucleation of K562 cells.


METHODS
K562 cells were grown in RPMI 1640 medium supplemented with 10% calf serum and 4 microL cytochalasin B (CB). Denucleation was induced in the cultured cells by CB, and the cells were examined by phase contrast microscopy and Giemsa staining respectively.


RESULTS
The denucleation of K562 cells induced by CB was clearly observed, and the cell proliferation was obviously inhibited.


CONCLUSIONS
The denucleation efficiency of K562 cells is positively correlated with CB concentrations and the duration of CB treatment. CB at low doses may inhibit the cell proliferation and at high doses causes cell death.
Wei
Min
M
Institute of Molecular Biology, First Military University, Guangzhou 510515, China.
Ma
Wen-Li
WL
Mao
Xiang-Ming
XM
Song
Yan-Bin
YB
Zhang
Bao
B
Feng
Chun-Qiong
CQ
Zheng
Wen-Ling
WL
D002455:Cell Division / Q000187:drug effects; D003571:Cytochalasin B / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020014:K562 Cells
D003571:Cytochalasin B