title: In vitro hydrodynamics of a decellularized pulmonary porcine valve, compared with a glutaraldehyde and polyurethane heart valve.
abstract: BACKGROUND
  Hydrodynamic performance of a decellularized pulmonary porcine valve was evaluated with a computer versatile pulse duplicator and compared to glutaraldehyde fixated stentless porcine bioprosthesis and a polyurethane heart valve.
  
  
  METHODS
  Decellularized pulmonary porcine matrices (Group I, n = 5) were treated chemically to become cell-free collagen matrices. The findings of this heart valve were compared with aortic glutaraldehyde treated porcine prostheses (Group II, n = 5) and polyurethane three leaflet valve prostheses (Group III, n = 1). Measurements were performed in 0.9% saline test fluid at room temperature. Measurements compared were closing time, closing volume, systemic pressure difference and energy losses. Each valve was measured 6 times with 70 beats/minute, a stroke volume of 70 ml corresponds to a cardiac output of 4.9 L/minute.
  
  
  RESULTS
  Group I and group III showed no significant differences between parameters. The measured closing time was significantly different (p < 0.001) between group I and II, respectively 24.333 and 53.600 ms and group II and III respectively 53.600 and 24.000. Difference in closing volume was significant (p < 0.05) between groups II and I respectively 3.67 and 0.68 ms and group II and III respectively 3.67 and 0.71. Systolic mean pressure gradient was 18.25 +/- 1.04 mm Hg in group II which was significantly different (p < 0.001) from groups I and III, respectively 10.65 +/- 0.29 mm Hg and 7.70 +/- 0.30 mm Hg.
  
  
  CONCLUSIONS
  Decellularized pulmonary porcine valves showed the same excellent performance as polyurethane valve prosthesis, which are superior to the investigated glutaraldehyde fixed xenograft.
authors.affiliation: Department of Cardiovascular Surgery, Charité, Humboldt University, Berlin, Germany. pascal.dohmen@charite.de
authors.forename: P M
authors.initials: PM
authors.lastname: Dohmen
authors.forename: M
authors.initials: M
authors.lastname: Scheckel
authors.forename: M
authors.initials: M
authors.lastname: Stein-Konertz
authors.forename: W
authors.initials: W
authors.lastname: Erdbruegger
authors.forename: K
authors.initials: K
authors.lastname: Affeld
authors.forename: W
authors.initials: W
authors.lastname: Konertz
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D005976:Glutaral; D006350:Heart Valve Prosthesis / Q000592:standards* / Q000639:trends; D008422:Materials Testing; D011336:Probability; D011474:Prosthesis Design; D011664:Pulmonary Valve; D011673:Pulsatile Flow; D012680:Sensitivity and Specificity; D013552:Swine; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D005976:Glutaral