title: Co-localisation of nitric oxide synthase and endothelin in the rat supraoptic nucleus.
abstract: The co-localisation of neuronal nitric oxide synthase and endothelin-1 was studied in the rat supraoptic nucleus at the electron microscopy level. Double pre-embedding immunocytochemistry was performed using ExtrAvidin-horseradish peroxidase and immunogold-silver techniques. Immunoreactivities to neuronal nitric oxide synthase and endothelin-1 were co-localised in sub-populations of endocrine neurones (cell bodies) and dendrites. Double-labelled axon terminals making asymmetrical synapses on unlabelled dendrites were also observed. The findings are discussed in terms of the possible role and significance of nitric oxide and endothlin-1 in the hypothalamo-neurohypophysial system.
authors.affiliation: Department of Anatomy and Developmental Biology and Centre for Neuroscience, University College London, Gower Street, London WCIE 6BT, UK.
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Mukherjee
authors.forename: Andrzej
authors.initials: A
authors.lastname: Loesch
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D019332:Endothelin-1 / Q000096:biosynthesis*; D000939:Epitopes; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D019581:Neuropil / Q000166:cytology / Q000378:metabolism; D009490:Neurosecretory Systems / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D052248:Nitric Oxide Synthase Type I; D051381:Rats; D017207:Rats, Sprague-Dawley; D013495:Supraoptic Nucleus / Q000201:enzymology / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D019332:Endothelin-1; D000939:Epitopes; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat