Cross-talk between Msx/Dlx homeobox genes and vitamin D during tooth mineralization.
Rickets is associated with site-specific disorders of enamel and dentin formation, which may reflect the impact of vitamin D on a morphogenetic pathway. This study is devoted to potential cross-talk between vitamin D and Msx/Dlx transcription factors. We raised the question of a potential link between tooth defects seen in mice with rickets and Msx2 gene misexpression, using mutant mice lacking the nuclear vitamin D receptor as an animal model. Our data showed a modulation of Msx2 expression. In order to search for a functional impact of this Msx2 misexpression secondary to rickets, we focused our attention on osteocalcin as a target gene for both vitamin D and Msx2. Combining Msx2 overexpression and vitamin D addition in vitro, we showed an inhibitory effect on osteocalcin expression in immortalized MO6-G3 odontoblasts. Finally, in the same cells, such combinations appeared to modulate VDR expression outlining the existence of complex cross-regulations between vitamin D and Msx/Dix pathways.
Lézot
F
F
Laboratoire de Biologie Orofaciale et Pathologie INSERM EMI 0110, Université Paris 7 EA2380, IFR 58 Institut des Cordeliers, rue de l'Ecole de Médecine, 75006 Paris, France. flezot@infobiogen.fr
Descroix
V
V
Mesbah
M
M
Hotton
D
D
Blin
C
C
Papagerakis
P
P
Mauro
N
N
Kato
S
S
MacDougall
M
M
Sharpe
P
P
Berdal
A
A
D000818:Animals; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005801:Genes, Homeobox / Q000502:physiology*; D018398:Homeodomain Proteins / Q000235:genetics*; D007180:Incisor / Q000503:physiopathology*; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics; D008822:Mice, Transgenic; D008903:Minerals / Q000378:metabolism*; D008963:Molar / Q000196:embryology; D009804:Odontoblasts / Q000378:metabolism; D015675:Osteocalcin / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000235:genetics; D012279:Rickets / Q000503:physiopathology*; D014157:Transcription Factors / Q000235:genetics*; D014807:Vitamin D / Q000378:metabolism*
D004268:DNA-Binding Proteins; C088334:Distal-less homeobox proteins; D018398:Homeodomain Proteins; C084119:MSX2 protein; D008903:Minerals; D012333:RNA, Messenger; D018167:Receptors, Calcitriol; D014157:Transcription Factors; D015675:Osteocalcin; D014807:Vitamin D