title: Down-regulation of an ankyrin repeat-containing protein, V-1, during skeletal muscle differentiation and its re-expression in the regenerative process of muscular dystrophy.
abstract: Using Western blot analysis and immunohistochemical methods, we examined the expression of V-1, a member of the ankyrin repeat-containing protein family, during differentiation and regeneration of skeletal muscle. The expression of V-1 was high in cultured myoblasts and decreased during their differentiation into myotubes, while high expression was maintained when muscle differentiation was inhibited by treatment with basic fibroblast growth factor. Down-regulation of V-1 also occurred during in vivo muscle differentiation from embryonic to postnatal stages, reaching an undetectable level in mature skeletal muscle. In contrast, strong V-1 immunoreactivity was detected again in myoblasts and regenerating muscle fibers with a small diameter, which were observed in Duchenne muscular dystrophy and its animal model, mdx mouse. Thus, it seems that V-1 is a good marker for early stage of muscle regeneration and changes of its expression suggest that V-1 plays a role in prenatal muscle differentiation and postnatal muscle regeneration.
authors.affiliation: Mitsubishi Kagaku Institute of Life Sciences, Minamiooya 11, Machida, 194-8511, Tokyo, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Furukawa
authors.forename: Naohiro
authors.initials: N
authors.lastname: Hashimoto
authors.forename: Tohru
authors.initials: T
authors.lastname: Yamakuni
authors.forename: Yukisato
authors.initials: Y
authors.lastname: Ishida
authors.forename: Chieko
authors.initials: C
authors.lastname: Kato
authors.forename: Masayo
authors.initials: M
authors.lastname: Ogashiwa
authors.forename: Masumi
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Ikuya
authors.initials: I
authors.lastname: Nonaka
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Mizusawa
authors.forename: Si Young
authors.initials: SY
authors.lastname: Song
mesh_terms: D000328:Adult; D000818:Animals; D017089:Ankyrin Repeat; D015153:Blotting, Western; D002454:Cell Differentiation; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D003893:Desmin / Q000378:metabolism; D003964:Diaphragm / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000378:metabolism / Q000473:pathology; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D009136:Muscular Dystrophies / Q000378:metabolism* / Q000473:pathology; D032446:Myoblasts / Q000378:metabolism; D018008:Myogenin / Q000378:metabolism; D009218:Myosins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology*
chemical_list: D003893:Desmin; D036341:Intercellular Signaling Peptides and Proteins; C408902:MHC binding peptide; C503799:MYOG protein, human; C503801:Myog protein, mouse; C503803:Myog protein, rat; D018008:Myogenin; D009419:Nerve Tissue Proteins; D009842:Oligopeptides; C065700:myotrophin; D017868:Cyclic AMP-Dependent Protein Kinases; D009218:Myosins