Effects of intraperitoneal and intranasal application of Lentinan on cellular response in rats.
Lentinan (Ajinomoto, Japan) was administrated intraperitoneally (i.p.) and intranasally (i.n.) at different doses (1, 5 and 10 mg/kg) to rats. Effectiveness of Lentinan treatment was evaluated by comparative testing of cell activation (establishing the number, glycolytic and acid phosphatase activity, H2O2 production and killing ability against Salmonella enteritidis and Staphylococcus aureus) at two different compartments--peritoneal and broncho-alveolar cavities. The results indicated that Lentinan induced high-grade activation of peritoneal cells (PCs) and especially of broncho-alveolar cells (BACs) with markedly enhanced effector function (killing ability against S. aureus). Generally, Lentinan, known usually with its parenteral routes of application, can be successful to stimulate the host cell response in the respiratory tract by intranasal route of administration.
Markova
Nadya
N
Department of Pathogenic Bacteria, Institute of Microbiology, Bulgarian Academy of Sciences, 26 G, Bonchev St., 1113 Sofia, Bulgaria. semahead@geobiz.net
Kussovski
Vesselin
V
Radoucheva
Tatyana
T
Dilova
Krasimira
K
Georgieva
Neli
N
D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000494:pharmacology*; D000281:Administration, Intranasal; D000818:Animals; D005260:Female; D007274:Injections, Intraperitoneal; D007912:Lentinan / Q000008:administration & dosage* / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects*; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000276:Adjuvants, Immunologic; D007912:Lentinan