Affinity chromatography system for parallel purification of recombinant protein samples.
In protein engineering, the tasks of generating and testing a large number of variants of a molecule and of optimizing expression conditions for one distinct molecule create the need for purification methods that can handle a large number of samples simultaneously. We describe the development and some application results of a simple affinity chromatography system that can be used for the parallel purification of 24 protein samples, yielding sufficient quantities for biochemical and functional analysis. Advantages of this system over existing systems are as follows. Compared with commercially available complete chromatography systems, the costs of this system are minimal. In comparison with vacuum systems with various outlets, and with batch purification systems where centrifugation is necessary, this system allows gentler processing of the samples. This could be important for proteins that are easily damaged.
Gottstein
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C
Department of Internal Medicine I, University of Cologne, 50924 Cologne and Aqua Research, 50974 Cologne, Germany.
Forde
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D002846:Chromatography, Affinity / Q000191:economics / Q000295:instrumentation* / Q000379:methods*; D005434:Flow Cytometry; D006639:Histidine / Q000737:chemistry; D000922:Immunotoxins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007930:Leucine / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D014316:Tritium
D000922:Immunotoxins; D011994:Recombinant Proteins; D014316:Tritium; D006639:Histidine; D007930:Leucine