title: DNA microarray analysis reveals novel gene expression profiles in collagen-induced arthritis.
abstract: Global gene expression was analyzed in early and late collagen-induced arthritis (CIA). Of 8734 cDNAs analyzed, 330 were induced and 55 downregulated greater than twofold in early or late disease. Hierarchical clustering of these 385 cDNAs demonstrated five distinct expression patterns differentiating early from late disease and correlating with histopathologic changes in the paw. Of the 385 cDNAs, 185 are known, characterized genes, the majority of which are not described as playing a role in arthritis. However, several of these genes are involved in pathological processes relating to arthritis, including apoptosis, inflammation, and cellular proliferation. One interesting gene, follistatin-like gene, is highly expressed along the margin of contact between inflammatory synovial pannus and eroding bone, suggesting a role in joint destruction. These results demonstrate that global gene expression profiles distinguish early and late CIA and reveal several genes novel to arthritis the further characterization of which will advance our understanding of arthritis.
authors.affiliation: William S. Rowe Division of Rheumatology, Children's Hospital Medical Center, Cincinnati, OH 45229, USA.
authors.forename: Sherry
authors.initials: S
authors.lastname: Thornton
authors.forename: Dawn
authors.initials: D
authors.lastname: Sowders
authors.forename: Bruce
authors.initials: B
authors.lastname: Aronow
authors.forename: David P
authors.initials: DP
authors.lastname: Witte
authors.forename: Hermine I
authors.initials: HI
authors.lastname: Brunner
authors.forename: Edward H
authors.initials: EH
authors.lastname: Giannini
authors.forename: Raphael
authors.initials: R
authors.lastname: Hirsch
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D015551:Autoimmunity / Q000235:genetics; D001483:Base Sequence; D016000:Cluster Analysis; D018076:DNA, Complementary / Q000235:genetics; D038702:Follistatin-Related Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D015375:Receptors, Interleukin-2 / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D018076:DNA, Complementary; D038702:Follistatin-Related Proteins; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2