[Cloning of human brain-derived neurotrophin-6 gene and its expression in procaryotic cell].
OBJECTIVE
To clone human brain-derived neurotrophin-6(NT-6) gene and to observe its expression in the procaryotic cell.


METHODS
Total RNA was extracted from aborted antenatal cerebral cortex, and cDNA fragment of NT-6 was amplified through reverse transcript-polymerase chain reaction. After being incised and recovered, the NT-6 gene was cloned into pBK-CMV plasmid to construct a NT-6 gene expression vector. Expression of NT-6 gene in Escherichia coli was studied after being induced by isopropyl beta-D-thiogalactoside(IPTG).


RESULTS
The NT-6 gene expression vector was constructed and Escherichia coli with recombinant vector expressed specific protein after induction by IPTG.


CONCLUSIONS
The cloning of human brain-derived NT-6 gene provides a basis for further studying the structure, function and clinical application of NT-6.
Zhang
Chengwu
C
Department of Physiology, School of Pre-clinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, 610041 P. R. China.
Cai
Qingsong
Q
Zhuang
Xu
X
Zhai
Chaoyang
C
Zheng
Yu
Y
D000595:Amino Acid Sequence; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D017434:Protein Structure, Tertiary; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D009414:Nerve Growth Factors; D011994:Recombinant Proteins; C075936:neurotrophin 6; D012313:RNA