Rolling-circle amplification in DNA diagnostics: the power of simplicity.
Due to its robustness and simplicity, the rolling replication of circular DNA probes holds a distinct position in DNA diagnostics among other isothermal methods of target, probe or signal amplification. Major rolling-circle amplification approaches to DNA detection via posthybridization probe/signal turn-by-turn enhancement are briefly overviewed here with an emphasis on the new concepts and latest progress in the field, including the single-molecule and single-mutation detection assays as exemplary applications. Underlying mechanisms, current controversies and principal advantages of rolling-circle amplification are also considered. Possible future directions for the further advancement of this diagnostic methodology are outlined.
Demidov
Vadim V
VV
Center for Advanced Biotechnology, Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA. vvd@bu.edu
D004252:DNA Mutational Analysis / Q000379:methods; D015342:DNA Probes / Q000737:chemistry*; D004261:DNA Replication; D004270:DNA, Circular / Q000096:biosynthesis* / Q000235:genetics; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005544:Forecasting; D005784:Gene Amplification; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008957:Models, Genetic; D009690:Nucleic Acid Conformation; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide; D013696:Temperature; D013698:Templates, Genetic
D015342:DNA Probes; D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004259:DNA-Directed DNA Polymerase