title: No effects of extremely low frequency magnetic fields found on cytotoxic activities and cytokine production of human peripheral blood mononuclear cells in vitro.
abstract: Several epidemiologic studies have suggested an association between exposure to extremely low frequency (ELF) magnetic fields (MFs) and cancer in adults and children. A possible target of MFs is the immune system. The effects of the exposure to ELF MFs on the immunological functions of human peripheral blood mononuclear cells (PBMCs) obtained from healthy male volunteers were assessed by measuring the natural killer (NK) and lymphokine activated killer (LAK) activities and the production of interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), interleukin-2 (IL-2), and interleukin-10 (IL-10). The PBMCs were exposed to three different MF: linearly polarized (vertical), circularly polarized, and elliptically polarized, at 50 and 60 Hz. Magnetic flux densities were set at 500, 100, 20, and 2 microT (rms) for vertical field and at 500 microT (rms) for the rotating fields. Using cytotoxicity assay and enzyme-linked immunosorbent assay (ELISA) for cytokine production, we could not find any effects of ELF MFs on the cytotoxic activities and the cytokines production of human PBMCs.
authors.affiliation: Technical Research Center, The Kansai Electric Power Company, Inc, Amagasaki, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ikeda
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shinmura
authors.forename: Hiroki
authors.initials: H
authors.lastname: Mizoe
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yoshizawa
authors.forename: Atsuya
authors.initials: A
authors.lastname: Yoshida
authors.forename: Seiichi
authors.initials: S
authors.lastname: Kanao
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sumitani
authors.forename: Setsu
authors.initials: S
authors.lastname: Hasebe
authors.forename: Toshio
authors.initials: T
authors.lastname: Motomura
authors.forename: Taku
authors.initials: T
authors.lastname: Yamakawa
authors.forename: Fumio
authors.initials: F
authors.lastname: Mizuno
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Otaka
authors.forename: Hideki
authors.initials: H
authors.lastname: Hirose
mesh_terms: D000328:Adult; D016207:Cytokines / Q000096:biosynthesis*; D003601:Cytotoxicity Tests, Immunologic; D004574:Electromagnetic Fields / Q000009:adverse effects*; D006801:Humans; D015979:Killer Cells, Lymphokine-Activated / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
chemical_list: D016207:Cytokines