Sensor and biosensor based on Prussian Blue modified gold and platinum screen printed electrodes.
Gold (Au) and platinum (Pt) screen-printed electrodes were modified with Prussian Blue (PB) for the development of amperometric sensors selective for hydrogen peroxide detection. The sensors exhibited sensitivities towards H(2)O(2) equal to 2 A M(-1) cm(-2) for Au and 1 A M(-1) cm(-2) for Pt electrodes. The sensors were also employed as the basis for construction of glucose biosensors through further modification with crystallised glucose oxidase immobilised in a Nafion membrane. In order to improve the operational stability of the modified electrodes a buffer solution containing tetrabutylammonium toluene-4-sulfonate was used. The long-term performance of the sensors and biosensors were evaluated by continuous monitoring of hydrogen peroxide and glucose solutions (50 microM and 1 mM, respectively) in the flow-injection mode for 10 h.
de Mattos
Ivanildo Luiz
IL
Department of Analytical Chemistry, University of Lund, P.O. Box 124, S-22100 Lund, Sweden.
Gorton
Lo
L
Ruzgas
Tautgirdas
T
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005295:Ferrocyanides / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000295:instrumentation; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D006046:Gold; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007854:Lead; D008422:Materials Testing; D011540:Prussian Blue Reaction / Q000295:instrumentation / Q000379:methods; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D005295:Ferrocyanides; D007854:Lead; D006046:Gold; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; C000170:ferric ferrocyanide