Excessive degradation of type II collagen in articular cartilage in equine osteochondrosis.
Articular osteochondrosis (OCD) occurs in both man and animals. The etiology remains to be determined. Studies of OCD lesions in animals may provide clues as to its pathogenesis. The aim of our study was to determine whether there was evidence for increased degradation namely proteoglycan (PG) release and type II collagen cleavage in articular cartilage harvested from OCD lesions. We examined ex vivo explants at post-mortem from equine OCD lesions and macroscopically normal site and age matched cartilage. These were cultured over a 10 day period in serum-free medium. Type II collagen cleavage was measured in articular cartilage and media using an Elisa assay to detect the COL2-3/4C(short) epitope, which is generated on cleavage of the triple helix of type II collagen by collagenases. PG release was measured by a dye-binding assay. Cumulative release of PG and COL2-3/4C(short) and their contents in cartilage at the end of the culture period were determined. In OCD lesions there was a significant increase in type II collagen cleavage by collagenase but no evidence for increase of PG degradation. These findings point to a selective increase in type II collagen cleavage by collagenases, in OCD lesions of the kind observed in osteoarthritis. Further work is needed to determine whether changes represent primary or secondary events in the pathogenesis of OCD.
Laverty
S
S
Faculté de Médecine Vétérinaire, Département de Sciences Cliniques, Université de Montreal, CP 5000, Saint Hyacinthe, Qué., Canada J2S 7C6. sheila.laverty@umontreal.ca
Okouneff
S
S
Ionescu
M
M
Reiner
A
A
Pidoux
I
I
Webber
C
C
Rossier
Y
Y
Billinghurst
R C
RC
Poole
A R
AR
D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D024043:Collagen Type II / Q000378:metabolism*; D017364:Collagenases; D003470:Culture Media; D004247:DNA / Q000032:analysis; D005260:Female; D006734:Horse Diseases / Q000378:metabolism* / Q000473:pathology; D006736:Horses; D008297:Male; D010007:Osteochondritis / Q000378:metabolism* / Q000473:pathology / Q000662:veterinary*; D011489:Protein Denaturation; D011509:Proteoglycans / Q000378:metabolism
D024043:Collagen Type II; D003470:Culture Media; D011509:Proteoglycans; D004247:DNA; D017364:Collagenases