A candidate X-linked mental retardation gene is a component of a new family of histone deacetylase-containing complexes.
Eukaryotic genes are under the control of regulatory complexes acting through chromatin structure to control gene expression. Here we report the identification of a family of multiprotein corepressor complexes that function through modifying chromatin structure to keep genes silent. The polypeptide composition of these complexes has in common a core of two subunits, HDAC1,2 and BHC110, an FAD-binding protein. A candidate X-linked mental retardation gene and the transcription initiation factor II-I (TFII-I) are components of a novel member of this family of complexes. Other subunits of these complexes include polypeptides associated with cancer causing chromosomal translocations. These findings not only delineate a novel class of multiprotein complexes involved in transcriptional repression but also reveal an unanticipated role for TFII-I in transcriptional repression.
Hakimi
Mohamed-Ali
MA
Wistar Institute, Philadelphia, Pennsylvania 19104, USA.
Dong
Yuanshu
Y
Lane
William S
WS
Speicher
David W
DW
Shiekhattar
Ramin
R
D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D002845:Chromatography; D041321:Chromosomes, Human, X; D008040:Genetic Linkage; D006367:HeLa Cells; D006655:Histone Deacetylases / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D008607:Intellectual Disability / Q000235:genetics*; D013058:Mass Spectrometry; D008957:Models, Genetic; D010455:Peptides / Q000737:chemistry; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D034741:RNA, Small Interfering / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic; D014162:Transfection; D014178:Translocation, Genetic
D002843:Chromatin; D010455:Peptides; D016760:Proto-Oncogene Proteins c-fos; D034741:RNA, Small Interfering; D006655:Histone Deacetylases