[Relationship of nitric oxide with intrauterine human cytomegalovirus active infection during early pregnancy].
OBJECTIVE
To study the relationship of nitric oxide (NO) with intrauterine human cytomegalovirus (HCMV) active infection during early pregnancy.


METHODS
Reverse transcript polymerase chain reaction (RT-PCR) was used to screen HCMV-mRNA of chorionic villi during early pregnancy in 81 cases who had poor pregnant history. The serum HCMV-IgM was measured by enzyme linked immunoadsorbent assay (ELISA) and the expression of decidual iNOS-mRNA was investigated with in situ hybridization (ISH).


RESULTS
(1) Six cases were HCMV-mRNA positive in chorionic villous tissues and 14 cases with serum HCMV-IgM positive among cases, the positive rate of serum HCMV-IgM was 17.3% (14/81). All cases were divided into three groups according to HCMV-mRNA and serum HCMV IgM. Group I: 5 cases with HCMV-mRNA positive and IgM positive, and the transmission rate between pregnant women and fetus was 35.7% (5/14); Group II: 9 cases with HCMV-mRNA negative and IgM positive, Group III: 66 cases with HCMV-mRNA and IgM negative; In addition, 1 case with HCMV-mRNA positive but IgM negative. (2) The expressive quantity of decidual iNOS-mRNA in group I was significantly higher than those in group II and III (P < 0.05). The case with HCMV-mRNA positive and serum IgM negative also has a strong staining of decidual iNOS-mRNA.


CONCLUSIONS
NO may play a critical role in intrauterine HCMV active infection, NO could be served as an assistant marker of HCMV infection.
Wang
Hui
H
Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
Wen
Liangzhen
L
Ling
Xiazhen
X
D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000209:etiology*; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D018445:Infectious Disease Transmission, Vertical; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis / Q000209:etiology*; D012333:RNA, Messenger / Q000032:analysis
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D012333:RNA, Messenger; D009569:Nitric Oxide; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II