Angiotensin II induces prostaglandin E(2) release in human gingival fibroblasts.
We investigated the effect of angiotensin II on prostaglandin E(2) release in human gingival fibroblasts. Stimulation of human gingival fibroblasts with angiotensin II elicited prostaglandin E(2) release in a concentration- and time-dependent manner. Angiotensin III also induced prostaglandin E(2) release, but the effect was weaker than that of angiotensin II. Angiotensin II- and angiotensin III-induced prostaglandin E(2) release was inhibited by AT(1) receptor antagonist FR-130,739, but not AT(2) receptor antagonist PD-123,319. Angiotensin II evoked an increase in intracellular Ca(2+) in fura-2-loaded human gingival fibroblasts. These results suggest that angiotensin II functions as a physiological mediator via Ca(2+)-mobilizing AT(1) receptor activation in human gingival fibroblasts.
Segawa
Masaomi
M
Department of Physiology, Nihon University School of Dentistry at Matsudo, Chiba 271-8587, Japan.
Nakao
Sumi
S
Ogata
Yorimasa
Y
Sugiya
Hiroshi
H
Furuyama
Shunsuke
S
D000804:Angiotensin II / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000805:Angiotensin III / Q000494:pharmacology; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D014662:Vasoconstrictor Agents / Q000494:pharmacology*
D014662:Vasoconstrictor Agents; D000804:Angiotensin II; D000805:Angiotensin III; D015232:Dinoprostone; D002118:Calcium