Characterisation of the genes encoding Aa1 isoforms from the scorpion Androctonus australis.
Aa1 is a toxin purified from the venom of the North African scorpion Androctonus australis. It blocks fast K(+) currents in cerebellar granular cells [Biochim. Biophys. Acta 1468 (2000) 203]. Two full-length cDNAs (about 250 bp) encoding the precursors of putative Aa1 isoforms (AaTX1 and AaTX2) were amplified by PCR from a venom gland cDNA library of A. australis. The deduced precursors were composed of 59 amino acid residues including a signal peptide of 22 residues and a mature toxin of 37 residues. The peptides display 94% sequence identity with Aa1. Intron-exon organisation of the gene corresponding to the AaTX1 cDNAs was also depicted.
Legros
Christian
C
UMR 6560 CNRS, Faculté de Médecine secteur Nord, Institut Fédératif de Recherche Jean Roche, Université de la Méditerranée, Bd Pierre Dramard, F-13916 Cedex 20, Marseille, France. chritian.legros@chimie.univ-evry.fr
Bougis
Pierre E
PE
Martin-Eauclaire
Marie-France
MF
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D007536:Isomerism; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012604:Scorpion Venoms / Q000737:chemistry / Q000235:genetics*; D012605:Scorpions
D017931:DNA Primers; D009498:Neurotoxins; D012604:Scorpion Venoms; C065314:scorpion toxin I, Androctonus