Analysis of Bacillus megaterium lipolytic system and cloning of LipA, a novel subfamily I.4 bacterial lipase.
The lipolytic system of Bacillus megaterium 370 was investigated, showing the existence of at least two secreted lipases and a cell-bound esterase. A gene coding for an extracellular lipase was isolated and cloned in Escherichia coli. The cloned enzyme displayed high activity on short to medium chain length (C(4)-C(8)) substrates, and poor activity on C(18) substrates. On the basis of amino acid sequence homology, the cloned lipase was classified into subfamily I.4 of bacterial lipases.
Ruiz
Cristian
C
Department of Microbiology, Faculty of Biology, University of Barcelona, Av Diagonal 645, 08028 Barcelona, Spain.
Blanco
Ana
A
Pastor
F I Javier
FI
Diaz
Pilar
P
D001410:Bacillus megaterium / Q000201:enzymology* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000235:genetics*; D008066:Lipolysis
D001426:Bacterial Proteins; C079999:LipA protein, Bacteria; D008049:Lipase