Characterization of a novel HLA-C allele, Cw*0315.
The presence of an unusual HLA class I reactivity pattern was detected in a Caucasoid-Asian individual by PCR-sequence specific primer (PCR-SSP) typing. Exons 2 and 3 were characterized using PCR-sequence-based typing (PCR-SBT) and were found to contain a novel Cw*03 sequence, Cw*0315. In the region studied, Cw*0315 was comprised mainly of the Cw*0302 sequence, but at four positions it contained nucleotides normally only found in other HLA Cw locus alleles. These positions each resulted in an amino acid substitution.
Chapple
S D J
SD
Transplant Immunology, Oxford Transplant Center, Churchill Hospital, Oxford, UK.
Agudelo
J D
JD
Barnardo
M C N M
MC
Vargas-Cuero
A L
AL
Fuggle
S V
SV
D001483:Base Sequence; D015236:HLA-C Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D016415:Sequence Alignment
D015236:HLA-C Antigens; C475486:HLA-C*03:15 antigen