title: The use of a thermally reversible bond for molecular imprinting of silica spheres.
abstract: A silica monomer-estrone complex (EstSi) having a thermally cleavable urethane bond and a cross-linkable triethoxysilane group was synthesized. From EstSi and TEOS, spherical silica particles with sizes of 1.5-3 mum were prepared. The template molecules were removed from the silica matrix by heating at 180 degrees C in DMSO in the presence of water, generating a cavity with an amino group. The control silica particles that had the same sizes and shapes were obtained with aminopropyl triethoxysilane and TEOS. When ethylene glycol was added in place of H2O, an ethyl alcoholic group was formed in the cavity. Their recognition ability and specific binding for estrone were characterized by uptake experiments. The estrone-imprinted silica particles showed a much higher recognition ability than the control silica particles and higher selectivity for estrone than testosterone propionate.
authors.affiliation: Hyperstructured Organic Materials Research Center, School of Materials Science and Engineering, Seoul National University, Seoul 151-744, Korea.
authors.forename: Chang Do
authors.initials: CD
authors.lastname: Ki
authors.forename: Chul
authors.initials: C
authors.lastname: Oh
authors.forename: Seong-Geun
authors.initials: SG
authors.lastname: Oh
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Chang