A defined human gastrin sequence stimulates the growth of Helicobacter pylori.
This study describes the interaction between gastrin and Helicobacter pylori. Human gastrin amino acids 4-17 were found to be the minimal growth-stimulating sequence. Gastrin from other mammals did not stimulate bacterial growth. When human serum was used to stimulate bacterial growth in brucella broth, gastrin was shown to be a necessary and sufficient growth-stimulating factor. Competition for the gastrin effect by pentagastrin and cholecystokinin (CCK-8) resulted in inhibition of bacterial growth. This effect was mediated by the four C-terminal amino acids which are shared by gastrin, CCK-8 and pentagastrin. In conclusion, the interaction between gastrin and H. pylori was shown to be specific, essential, and dependent on a defined gastrin sequence.
Chowers
Michal Yael
MY
Infectious Disease Unit, Meir Hospital, Sapir Medical Center, Kfar Saba 44281, Israel. chowersm@post.tau.ac.il
Keller
Nathan
N
Bar-Meir
Simon
S
Chowers
Yehuda
Y
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000145:classification / Q000378:metabolism; D005755:Gastrins / Q000737:chemistry* / Q000494:pharmacology*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development*; D006801:Humans; D008969:Molecular Sequence Data; D010418:Pentagastrin / Q000378:metabolism
D000596:Amino Acids; D005755:Gastrins; C472485:gastrin (4-17); D010418:Pentagastrin