Erythropoietin improves synaptic transmission during and following ischemia in rat hippocampal slice cultures.
Erythropoietin (EPO) prevents neuronal damage following ischemic, metabolic, and excitotoxic stress. In this study evoked extracellular field potentials (FP) were used to investigate the effect of EPO on synaptic transmission in hippocampal slice cultures. EPO treated cultured slices (40 units/ml for 48 h) showed significantly increased FP during and following oxygen and glucose deprivation compared with untreated control slices. The addition of the Jak2 inhibitor AG490 (50 microM for 48 h) blocked the EPO effect. These data suggest that EPO improves synaptic transmission during and following ischemia in hippocampal slice cultures.
Weber
Astrid
A
Department of Neonatology, Charité, Humboldt University Berlin, Campus Virchow-Klinikum, Augustenburger Platz 1, D-13353 Berlin, Germany. astrid.weber@charite.de
Maier
Rolf F
RF
Hoffmann
Ulrike
U
Grips
Martin
M
Hoppenz
Marc
M
Aktas
Ayse G
AG
Heinemann
Uwe
U
Obladen
Michael
M
Schuchmann
Sebastian
S
D000818:Animals; D015687:Cell Hypoxia / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004921:Erythropoietin / Q000096:biosynthesis / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D066298:In Vitro Techniques; D053614:Janus Kinase 2; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D011518:Proto-Oncogene Proteins; D051381:Rats; D017208:Rats, Wistar; D017467:Receptors, Erythropoietin / Q000096:biosynthesis; D009435:Synaptic Transmission / Q000187:drug effects* / Q000502:physiology
D004791:Enzyme Inhibitors; D011518:Proto-Oncogene Proteins; D017467:Receptors, Erythropoietin; D004921:Erythropoietin; D011505:Protein-Tyrosine Kinases; C507927:Jak2 protein, rat; D053614:Janus Kinase 2