Down-regulation of an ankyrin repeat-containing protein, V-1, during skeletal muscle differentiation and its re-expression in the regenerative process of muscular dystrophy.
Using Western blot analysis and immunohistochemical methods, we examined the expression of V-1, a member of the ankyrin repeat-containing protein family, during differentiation and regeneration of skeletal muscle. The expression of V-1 was high in cultured myoblasts and decreased during their differentiation into myotubes, while high expression was maintained when muscle differentiation was inhibited by treatment with basic fibroblast growth factor. Down-regulation of V-1 also occurred during in vivo muscle differentiation from embryonic to postnatal stages, reaching an undetectable level in mature skeletal muscle. In contrast, strong V-1 immunoreactivity was detected again in myoblasts and regenerating muscle fibers with a small diameter, which were observed in Duchenne muscular dystrophy and its animal model, mdx mouse. Thus, it seems that V-1 is a good marker for early stage of muscle regeneration and changes of its expression suggest that V-1 plays a role in prenatal muscle differentiation and postnatal muscle regeneration.
Furukawa
Yuko
Y
Mitsubishi Kagaku Institute of Life Sciences, Minamiooya 11, Machida, 194-8511, Tokyo, Japan.
Hashimoto
Naohiro
N
Yamakuni
Tohru
T
Ishida
Yukisato
Y
Kato
Chieko
C
Ogashiwa
Masayo
M
Kobayashi
Masumi
M
Kobayashi
Takayoshi
T
Nonaka
Ikuya
I
Mizusawa
Hidehiro
H
Song
Si Young
SY
D000328:Adult; D000818:Animals; D017089:Ankyrin Repeat; D015153:Blotting, Western; D002454:Cell Differentiation; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D003893:Desmin / Q000378:metabolism; D003964:Diaphragm / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000378:metabolism / Q000473:pathology; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D009136:Muscular Dystrophies / Q000378:metabolism* / Q000473:pathology; D032446:Myoblasts / Q000378:metabolism; D018008:Myogenin / Q000378:metabolism; D009218:Myosins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology*
D003893:Desmin; D036341:Intercellular Signaling Peptides and Proteins; C408902:MHC binding peptide; C503799:MYOG protein, human; C503801:Myog protein, mouse; C503803:Myog protein, rat; D018008:Myogenin; D009419:Nerve Tissue Proteins; D009842:Oligopeptides; C065700:myotrophin; D017868:Cyclic AMP-Dependent Protein Kinases; D009218:Myosins