Interleukin-12 inhibits apoptosis in chronic lymphatic leukemia (CLL) B cells.
The purpose of the present study was to investigate the effect of interleukin-12 on apoptosis of chronic lymphatic leukemia (CLL) B cells. Apoptotic indices were determined in highly purified CD5(+) B lymphocytes isolated from peripheral blood of seven patients with histologically confirmed CLL. Interleukin-4 as a known inhibitor of apoptosis was used as control. Quantitative analysis of apoptosis was determined by cell death detection ELISA. Our findings indicate that interleukin-12 inhibits ex vivo apoptosis in a large proportion of B-CLL patients and may be closely involved in the pathogenesis of disease. Therefore, our results may help identify potential new therapeutic targets in this malignancy.
Schuhknecht
S
S
Medizinische Hochschule Hannover, Hämatologie und Onkologie, Hannover, Germany.
Duensing
S
S
Dallmann
I
I
Grosse
J
J
Reitz
M
M
Atzpodien
J
J
D000328:Adult; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D005260:Female; D006801:Humans; D018664:Interleukin-12 / Q000494:pharmacology* / Q000627:therapeutic use; D015847:Interleukin-4; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D008875:Middle Aged
D018664:Interleukin-12; D015847:Interleukin-4