title: Variability of hydrocarbon and fatty acid components in cultures of the filamentous cyanobacterium Scytonema sp. isolated from microbial community "black cover" of limestone walls in Jerusalem.
abstract: The hydrocarbon and lipid components of four strains of the filamentous cyanobacterium Scytonema sp. isolated from microbial community "Black Cover" of limestone walls in Jerusalem were identified by gas chromatography-mass spectrometry using serially coupled capillary columns. The dominant compounds were: 1-heptadecyne (1.5-8%), hexadecanoic acid (14-36%), (Z,Z)-9,12-octadecadienoic acid (12-30%), (Z,Z,Z)-9,12,15-octadecatrienoic acid (6-12%), n-heptadecane (4-16%), and 1-heptadecene (1.5-8%). In addition to unsaturated alkanes and fatty acids, the very long-chain (C30-C32) hydrocarbons, squalene (2.4-3.0%), and branched 4,8,12-trimethyl-C13:0 acid were also isolated. Two major hydrocarbons were detected in the cyanobacteria species using GC-MS and 13C-NMR.
authors.affiliation: Department of Medicinal Chemistry and Natural Products, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem, 91120, Israel. dvalery@cc.huji.ac.il
authors.forename: V M
authors.initials: VM
authors.lastname: Dembitsky
authors.forename: M
authors.initials: M
authors.lastname: Srebnik
mesh_terms: D000473:Alkanes / Q000737:chemistry / Q000302:isolation & purification; D018441:Biofilms; D002119:Calcium Carbonate; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D004783:Environmental Microbiology; D005227:Fatty Acids / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006838:Hydrocarbons / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D007536:Isomerism; D007557:Israel; D013185:Squalene / Q000302:isolation & purification
chemical_list: D000473:Alkanes; D005227:Fatty Acids; D006838:Hydrocarbons; D013185:Squalene; D002119:Calcium Carbonate