title: Transepithelial ultrafiltration and fractal power diffusion of D-glucose in the perfused rat intestine.
abstract: Despite an enormous body of research investigating the mass transfer of D-glucose through biological membranes, carrier-mediated and first-order models have remained the prevalent models describing glucose's quantitative behavior even though they have proven to be inadequate over extended concentration ranges. Recent evidence from GLUT2 knockout studies further questions our understanding of molecular models, especially those employing Michaelis-Menten (MM)-type kinetic models. In this report, evidence is provided that D-glucose is absorbed by rat intestinal epithelium by a combination of convective ultrafiltration and nonlinear diffusion. The diffusive component of mass transfer is described by a concentration-dependent permeability coefficient, modeled as a fractal power function. Glucose and sodium chloride-dependent-induced aqueous convection currents are the result of prevailing oncotic and osmotic pressure effects, and a direct effect of glucose and sodium chloride on intestinal epithelium resulting in enhanced glucose, sodium ion, and water mobility. The fractal power model of glucose diffusion was superior to the conventional MM description. A convection-diffusion model of mass transfer adequately characterized glucose mass transfer over a 105-fold glucose concentration range in the presence and absence of sodium ion.
authors.affiliation: Division of Pharmacal Sciences, Auburn University School of Pharmacy, 401 Walker Pharmacy Building, Auburn University, AL 36849-5503, USA. kochagm@auburn.edu
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Kochak
authors.forename: Surinder
authors.initials: S
authors.lastname: Mangat
mesh_terms: D000818:Animals; D004058:Diffusion; D017709:Fractals; D005947:Glucose / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D051381:Rats; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D005947:Glucose