Comparison of a putative second serotype of chicken infectious anemia virus with a prototypical isolate II. Antigenic and physicochemical characteristics.
A putative new serotype of chicken infectious anemia virus (CIAV) isolated from 17-wk-old broiler breeder pullets was compared with a known, previously characterized CIAV isolate, the Del-Ros strain. Physicochemical characteristics evaluated induded thermal stability, size, pH, and chloroform sensitivity. Physicochemically, CIAV-7 was identical to CIAV. The virus isolates were compared antigenically by enzyme-linked immunosorbent assay, virus neutralization, immunofluorescence assay, and western blot. All four serologic assays demonstrated that CIAV-7 is antigenically distinct from the Del-Ros strain of CIAV. Additionally, polymerase chain reaction (PCR) and Southern blot were used to determine if there were similarities in genome sequence between the two viruses. CIAV-7 could not be detected with CIAV-specific PCR primers or a with CIAV-specific probe by Southern hybridization.
Spackman
E
E
Department of Animal and Food Sciences, 044 Townsend Hall, University of Delaware, Newark, DE 19717, USA.
Cloud
S S
SS
Rosenberger
J K
JK
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology; D002478:Cells, Cultured; D017727:Chicken anemia virus / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D002645:Chickens; D018173:Circoviridae Infections / Q000503:physiopathology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D009500:Neutralization Tests / Q000662:veterinary; D011201:Poultry Diseases / Q000503:physiopathology / Q000821:virology*; D013045:Species Specificity
D000914:Antibodies, Viral; D000956:Antigens, Viral