Arg tyrosine kinase is involved in homologous recombinational DNA repair.
c-Abl plays important roles in cellular response to DNA damage. However, possible roles for Arg (Abl-related gene) in DNA damage response are unknown. Here, we show that ionizing radiation (IR)-induced Rad51 focus formation is reduced in Arg-deficient cells generated from a chicken B cell line by targeted disruption. This is consistent with the findings that Arg-deficient cells display hypersensitivity to IR, elevated frequencies of IR-induced chromosomal aberrations, and reduced targeted integration frequencies. All of these abnormalities in DNA damage repair are also observed in ATM-deficient cells but not in c-Abl-deficient cells. Finally, we show that Arg interacts with and phosphorylates Rad51 in 293T cells. These results suggest that Arg plays a role in homologous recombinational (HR) DNA repair by phosphorylating Rad51.
Li
Yingzhu
Y
Department of Molecular Pathology, Cancer Research Institute, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa 920-0934, Japan.
Shimizu
Hiroko
H
Xiang
Shuang-Lin
SL
Maru
Yoshiro
Y
Takao
Noriaki
N
Yamamoto
Ken-ichi
K
D000818:Animals; D030161:Avian Proteins; D002460:Cell Line; D002470:Cell Survival; D002645:Chickens; D002869:Chromosome Aberrations; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000378:metabolism; D018390:Gene Targeting; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000502:physiology*; D051135:Rad51 Recombinase; D011839:Radiation, Ionizing; D011995:Recombination, Genetic
D030161:Avian Proteins; D004268:DNA-Binding Proteins; C426236:ARG tyrosine kinase; D011505:Protein-Tyrosine Kinases; C495150:RAD51 protein, Gallus gallus; D051135:Rad51 Recombinase