title: Effects of selenium and iodine on the expression of c-fos and c-jun mRNA and their proteins in cultured rat hippocampus neurons.
abstract: OBJECTIVE
  To study the effect of selenium (Se) and iodine (I) and the compound of both on the proto-oncogenes c-fos and c-jun mRNA and their protein expression in the cultured rat hippocampus neurons.
  
  
  METHODS
  Using the technique of serum free hippocampus neuron culture, different doses of Se and I and Se + I compound were added into the medium. The expression of the mRNA of c-fos, c-jun in hippocampus neurons cultured for 1, 3, 5, 7 and 10 d were studied using both in situ hybridization and SABC immunohistochemical technique.
  
  
  RESULTS
  Both Se and I could enhance the expression of c-fos, c-jun mRNA and their proteins, especially the combination of I and Se able to give a remarkable effect on c-jun mRNA expression.
  
  
  CONCLUSIONS
  Se and I may effect the expression of both c-fos and c-jun mRNA, especially the c-jun mRNA and its protein of hippocampus neurons, and thus may effect the differentiation and development of neurons.
authors.affiliation: Department of Pathology, Shantou University Medical College, Shantou 515031, China (Email: minsu@ncrc.stu.edu.cn)
authors.forename: Dongping
authors.initials: D
authors.lastname: Tian
authors.forename: Min
authors.initials: M
authors.lastname: Su
authors.forename: Xianying
authors.initials: X
authors.lastname: Wu
authors.forename: Qiaoshan
authors.initials: Q
authors.lastname: Li
authors.forename: Ruiming
authors.initials: R
authors.lastname: Zheng
authors.forename: Guangyuan
authors.initials: G
authors.lastname: Li
authors.forename: Tianbao
authors.initials: T
authors.lastname: Song
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Xu
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D007455:Iodine; D009474:Neurons / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012643:Selenium
chemical_list: D004268:DNA-Binding Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D007455:Iodine; D012643:Selenium