title: Magnification chromoendoscopy for the detection of intestinal metaplasia and dysplasia in Barrett's oesophagus.
abstract: BACKGROUND
  The presence of intestinal metaplasia (IM) in the columnar lined distal oesophagus defines Barrett's oesophagus with the risk of future malignant transformation. The distribution of both IM and dysplasia (low grade (LGD) and high grade (HGD)) within the columnar lined oesophagus is patchy and mosaic requiring random biopsies. Techniques that could help target areas of high yield within Barrett's mucosa would be helpful.
  
  
  OBJECTIVE
  To study the utility of high magnification chromoendoscopy (MCE) in the detection of IM, LGD, and HGD in patients with Barrett's oesophagus.
  
  
  METHODS
  Consecutive patients detected with columnar mucosa in the distal oesophagus were studied using an Olympus magnification endoscope (GIF-Q16OZ, 115x). The distal oesophagus was sprayed with indigo carmine solution and the oesophageal columnar mucosa patterns were noted under high magnification and targeted for biopsy. All biopsies were read by pathologists blinded to the endoscopic findings.
  
  
  RESULTS
  Eighty patients with suspected Barrett's oesophagus (that is, columnar lined distal oesophagus) were studied: mean age 62.7 years (range 35-81). Mean length of columnar mucosa was 3.7 cm (range 0.5-17). Three types of mucosal patterns were noted within the columnar mucosa after spraying indigo carmine and using MCE: ridged/villous pattern, circular pattern, and irregular/distorted pattern. The yield of IM on target biopsies according to the patterns was: ridged/villous 57/62 (97%) and circular 2/12 (17%). Six patients had an irregular/distorted pattern and all had HGD on biopsy (6/6 (100%)). Eighteen patients had LGD on target biopsies; all had the ridged/villous pattern. All patients with long segment Barrett's were identified using MCE whereas 23/28 patients (82%) with short segment Barrett's had the ridged/villous pattern.
  
  
  CONCLUSIONS
  MCE helps visually identify areas with IM and HGD having specific patterns but not patients with LGD (appear similar to IM). MCE may be a useful clinical tool for the increased detection of patients with IM as well as for surveillance of patients for the detection of HGD. If these preliminary results are validated, MCE would help identify high yield areas, potentially eliminating the need for random biopsies.
authors.affiliation: Department of Medicine, Gastroenterology, and Pathology Section, University of Kansas School Medicine and Veterans Affairs Medical Center, Kansas City, MO, USA. psharma@kumc.edu
authors.forename: P
authors.initials: P
authors.lastname: Sharma
authors.forename: A P
authors.initials: AP
authors.lastname: Weston
authors.forename: M
authors.initials: M
authors.lastname: Topalovski
authors.forename: R
authors.initials: R
authors.lastname: Cherian
authors.forename: A
authors.initials: A
authors.lastname: Bhattacharyya
authors.forename: R E
authors.initials: RE
authors.lastname: Sampliner
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001471:Barrett Esophagus / Q000473:pathology*; D004396:Coloring Agents; D004938:Esophageal Neoplasms / Q000473:pathology*; D004945:Esophagoscopy / Q000379:methods*; D004947:Esophagus / Q000473:pathology*; D005260:Female; D006801:Humans; D007203:Indigo Carmine; D007422:Intestines / Q000473:pathology; D008297:Male; D008679:Metaplasia; D008875:Middle Aged; D009092:Mucous Membrane / Q000473:pathology; D011230:Precancerous Conditions / Q000473:pathology*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D004396:Coloring Agents; D007203:Indigo Carmine