Separation of ephedrine stereoisomers by molecularly imprinted polymers--influence of synthetic conditions and mobile phase compositions on the chromatographic performance.
Separation of ephedrine stereoisomers by molecularly imprinted polymers was performed to study the factors that affect the selectivity and column efficiency. The polymer synthesized with pentaerythritol triacrylate as the cross-linker and chloroform as the porogen was found to have the best overall separation performance. Investigation of the recognition mechanism by NMR and chromatographic analysis revealed that the major binding forces between the polymer stationary phase and ephedrine are the ionic and hydrogen bonding interactions. Studies of the influence of mobile phase compositions on the HPLC analysis have shown that a methanol-aqueous buffer was the suitable mobile phase for the separation in which pH, ionic strength and water content can be adjusted to optimize the chromatographic analysis.
Dong
Xiangchao
X
Department of Chemistry, Nankai University, Tianjin, China. xcdong@nankai.edu.cn
Sun
Hui
H
Lu
Xianyu
X
Wang
Haibo
H
Liua
Shuxia
S
Wang
Ning
N
D018663:Adrenergic Agents / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004809:Ephedrine / Q000032:analysis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates; D011108:Polymers; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D018663:Adrenergic Agents; D008689:Methacrylates; D011108:Polymers; C008384:methacrylic acid; D004809:Ephedrine