title: Regulation of lymphocyte clustering by CD30-mediated ICAM-1 up-regulation.
abstract: CD30 is expressed transiently on activated B and T lymphocytes and constitutively on several B- and T cell lymphomas. CD30 functions include participation in negative selection of thymocytes, costimulation of activated T cells, isotype switching of B cells, and regulation of the effector activity of cytotoxic lymphocytes. Although CD30 is not a marker for T helper 2 (TH2) cells, it may participate in the polarization of TH1 and TH2 cells. The pleiotropic functions of CD30 are initiated by interaction of CD30-expressing cells with other immune competent cells expressing CD30-L and providing the signals for modulation of effector cell activity. Here, we report that CD30 signals generated by anti-CD30 on activated, normal murine T cells strongly up-regulate the expression of intercellular adhesion molecule 1 (ICAM-1, CD54), and to a lesser extent, ICAM-2 (CD102). CD30 signals moreover delay the subsequent decline of ICAM expression. CD30 cross-linking did not alter the expression of CD11a/CD18 (LFA-1), the counter receptor for ICAM abundant on T cells. CD30-mediated ICAM-1 up-regulation is independent of cytokine secretion and appears to be transmitted directly through NF-kappaB activation. CD30-mediated up-regulation of ICAM-1 expression led to a significant increase in cluster formation of lymph node cells. Increased lymphocyte self-aggregation mediated by CD30 may set the stage for fraternal signaling to modulate lymphocyte function.
authors.affiliation: Department of Microbiology, School of Natural Science, Jeonju University, 560-759, Jeonju, Republic of Korea.
authors.forename: Sang Yun
authors.initials: SY
authors.lastname: Nam
authors.forename: Kyong Shin
authors.initials: KS
authors.lastname: Cho
authors.forename: Young Moon
authors.initials: YM
authors.lastname: Heo
authors.forename: Jong Cheon
authors.initials: JC
authors.lastname: Ha
authors.forename: Young Hyun
authors.initials: YH
authors.lastname: Kim
authors.forename: Ho
authors.initials: H
authors.lastname: Keun Yi
authors.forename: Pyong
authors.initials: P
authors.lastname: Han Hwang
authors.forename: Hyung Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Eckhard R
authors.initials: ER
authors.lastname: Podack
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D017730:Ki-1 Antigen / Q000096:biosynthesis* / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000494:pharmacology; D015398:Signal Transduction; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D016207:Cytokines; C065289:ICAM2 protein, human; D017730:Ki-1 Antigen; D016169:Lymphocyte Function-Associated Antigen-1; D016328:NF-kappa B; D018799:Intercellular Adhesion Molecule-1