Insulin-like growth factor I administration in young rats with acute renal failure.
The outcome of ischemic acute renal failure (IARF) is better in young than adult rats. Insulin-like growth factor I (IGF-I) treatment may increase mortality of adult rats with IARF, probably because of an exaggerated inflammatory response. We report the response to IGF-I therapy in young rats with IARF. Male rats, aged 28+/-1 days, with IARF were given subcutaneous IGF-I, 50 microg/100 g at 0, 8, and 16 h after reperfusion (IGF) or were untreated (ARF). Sham-operated rats were used as controls. At 2 and 7 days after ischemia, serum urea nitrogen and histological damage score, cell proliferation, apoptosis, neutrophil infiltration, and IGF-I receptor mRNA in kidneys were analyzed. The degree of renal failure, mortality rate, histological damage, cell proliferation, and neutrophil infiltration were not different between IGF-I and ARF rats. Hence, short-term IGF-I treatment did not modify the course of IARF in young rats.
Medina
Alberto
A
Department of Pediatrics, Hospital Central de Asturias, SESPA and School of Medicine, University of Oviedo, Oviedo, Asturias, Spain.
Fernández-Fuente
Marta
M
Carbajo-Pérez
Eduardo
E
Santos
Fernando
F
Amil
Benito
B
Rodríguez
Julián
J
Crespo
Manuel
M
D058186:Acute Kidney Injury / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D000818:Animals; D000963:Antimetabolites; D017209:Apoptosis / Q000502:physiology; D001806:Blood Urea Nitrogen; D001973:Bromodeoxyuridine; D002455:Cell Division / Q000502:physiology; D017403:In Situ Hybridization; D007334:Insulin-Like Growth Factor I / Q000627:therapeutic use*; D007668:Kidney / Q000473:pathology; D007677:Kidney Function Tests; D008297:Male; D020556:Neutrophil Infiltration; D023282:Nuclease Protection Assays; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D016019:Survival Analysis
D000963:Antimetabolites; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D001973:Bromodeoxyuridine