A re-evaluation of the contributions of Apterous and Notch to the dorsoventral lineage restriction boundary in the Drosophila wing.
The Drosophila limb primordia are subdivided into compartments: cell populations that do not mix during development. The wing is subdivided into dorsal (D) and ventral (V) compartments by the activity of the selector gene apterous in D cells. Apterous causes segregation of D and V cell populations by at least two distinct mechanisms. The LRR transmembrane proteins Capricious and Tartan are transiently expressed in D cells and contribute to initial segregation of D and V cells. Signaling between D and V cells mediated by Notch and Fringe contributes to the maintenance of the DV affinity boundary. Given that Notch is activated symmetrically, in D and V cells adjacent to the boundary, its role in boundary formation remains somewhat unclear. We re-examine the roles of Apterous and Fringe activities in DV boundary formation and present evidence that Fringe cannot, by itself, generate an affinity difference between D and V cells. Although not sufficient, Fringe is required via Notch activation for expression of an Apterous-dependent affinity difference. We propose that Apterous controls expression of surface proteins that confer an affinity difference in conjunction with activated Notch. Thus, we view Apterous as instructive and Notch activity as essential, but permissive.
Milán
Marco
M
European Molecular Biology Laboratory, Meyerhofstr 1, 69117 Heidelberg, Germany.
Cohen
Stephen M
SM
D000483:Alleles; D000818:Animals; D019521:Body Patterning / Q000235:genetics / Q000502:physiology; D002450:Cell Communication; D004330:Drosophila / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D029721:Drosophila Proteins / Q000235:genetics* / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D018398:Homeodomain Proteins / Q000235:genetics* / Q000502:physiology; D060850:LIM-Homeodomain Proteins; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D008954:Models, Biological; D009154:Mutation; D051880:Receptors, Notch; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology; D014921:Wings, Animal / Q000166:cytology / Q000254:growth & development*
D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; D008565:Membrane Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch; D014157:Transcription Factors; C074459:ap protein, Drosophila