title: [Application of Agilent 2100 Bioanalyzer in the study of differential gene expression].
abstract: OBJECTIVE
  To study the application of Agilent 2100 Bioanalyzer in the study of gene differential expression.
  
  
  METHODS
  The total RNAs were extracted and purified from Saccharomyces cerevisiae to synthesize double-stranded cDNAs by reverse transcription. Restriction display-PCR was employed to obtain the cDNA fragments, which were examined by Agilent 2100 Bioanalyzer and agarose gel electrophoresis.
  
  
  RESULTS
  The analysis showed that Agilent 2100 Bioanalyzer was more sensitive and faster to isolate and display the differentially expressed genes, providing at the same time accurate quantitative information for each fragment in the DNA sample.
  
  
  CONCLUSIONS
  Agilent 2100 Bioanalyzer can be instrumental for the study of differential gene expression.
authors.affiliation: Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.
authors.forename: Cui-Hua
authors.initials: CH
authors.lastname: Liu
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Ma
authors.forename: Rong
authors.initials: R
authors.lastname: Shi
authors.forename: Qian
authors.initials: Q
authors.lastname: Ouyang
authors.forename: Wen-Ling
authors.initials: WL
authors.lastname: Zheng
mesh_terms: D018076:DNA, Complementary / Q000032:analysis; D004587:Electrophoresis, Agar Gel / Q000379:methods; D015870:Gene Expression; D020869:Gene Expression Profiling; D006360:Heat-Shock Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D018076:DNA, Complementary; D006360:Heat-Shock Proteins