title: Determination of ciprofloxacin with a room-temperature phosphorescence flow-through sensor based on lanthanide-sensitized luminescence.
abstract: Direct measurement of the sensitized luminescence of the europium-ciprofloxacin chelate immobilized on a cationic exchanger was used to develop a flow-through room-temperature phosphorescence optosensor for determination of ciprofloxacin. The phosphorescent chelate is formed on-line at room temperature in a flow-injection system and is immobilized on a weakly acidic cation-exchange resin packed in a flow cell. Optimum experimental conditions and analytical performance are discussed in detail. The sensor response for ciprofloxacin was linear, from 1.5 x 10(-6) to 2.0 x 10(-5)M with a relative standard deviation of 2.5% (n = 10) and a detection limit of 1.1 x 10(-7)M. The effect of possible interferences on sensor response was studied. The sensor was successfully tested for the determination of ciprofloxacin in pharmaceutical formulations.
authors.affiliation: University of Jaén, Department of Physical and Analytical Chemistry, Spain.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Cuenca-Trujillo
authors.forename: M José
authors.initials: MJ
authors.lastname: Ayora-Cañada
authors.forename: Antonio
authors.initials: A
authors.lastname: Molina-Díaz
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis*; D002939:Ciprofloxacin / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D028581:Lanthanoid Series Elements; D008163:Luminescent Measurements; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000890:Anti-Infective Agents; D007202:Indicators and Reagents; D028581:Lanthanoid Series Elements; D012997:Solvents; D002939:Ciprofloxacin