title: The expression of cyclooxygenase-2 in human temporomandibular joint samples: an immunohistochemical study.
abstract: The aim of this investigation was to evaluate the immunohistochemical expression of cyclooxygenase-2 (COX-2) in the temporomandibular joint (TMJ) and to compare it with that control specimens. Expression of COX-2 in the TMJ disc and the synovial membrane in 26 human TMJ samples (internal derangement of TMJ; n=16, and control; n=10) was measured by an immunohistological technique using paraffin-embedded tissue and specific antihuman COX-2 polyclonal antibody. There were obvious distinction of COX-2 immunoreactivity between the control specimens and internal derangement cases, in the region of posterior and/or anterior loose connective tissues. In particular, intensive COX-2 expression was detected in the synovial membrane of internal derangement cases. The findings of the present study suggest that COX-2 might be an important mechanism regulating inflammation in the synovial membrane with internal derangement of TMJ.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Fukui Prefectural Hospital, Fukui, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: Y
authors.initials: Y
authors.lastname: Fukumura
authors.forename: S
authors.initials: S
authors.lastname: Fujita
authors.forename: M
authors.initials: M
authors.lastname: Nishida
authors.forename: T
authors.initials: T
authors.lastname: Iizuka
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D051546:Cyclooxygenase 2; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis*; D013583:Synovial Membrane / Q000737:chemistry; D013704:Temporomandibular Joint / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D008565:Membrane Proteins; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases