Limited growth of severed CNS axons after treatment of adult rat brain with hyaluronidase.
Many chondroitin sulfate proteoglycans (CSPGs) have been shown to influence CNS axon growth in vitro and in vivo. These interactions can be mediated through the core protein or through the chondroitin sulfate (CS) glycosaminoglycan (GAG) side chains. We have shown previously that degrading CS GAG side chains using chondroitinase ABC enhances dopaminergic nigrostriatal axon regeneration in vivo. We test the hypothesis that interfering with complete CSPGs also limit axon growth in vivo. Neurocan, versican, aggrecan, and brevican CSPGs may be anchored within extracellular matrix through binding to hyaluronan glycosaminoglycan. We examine whether degradation of hyaluronan using hyaluronidase might release these inhibitory CSPGs from the extracellular matrix and thereby enhance regeneration of cut nigrostriatal axons. Anesthetized adult rats were given knife cut lesions of the right hemisphere nigrostriatal tract and cannulae were secured transcranially thereby allowing repeated perilesional infusion of saline or saline containing hyaluronidase once daily for 10 days post-axotomy. Eleven days post-transection brains from animals under terminal anesthesia were recovered for histological evaluation. Effective delivery of substance was inferred from the observed reduction in perilesional immunoreactivity for neurocan and versican after treatment with hyaluronidase (relative to saline). Immunolabeling using antibodies against tyrosine hydroxylase was used to examine the response of cut dopaminergic nigral neurons. After transection and treatment with saline, dopaminergic nigral neurons sprouted in a region lacking astrocytes, neurocan and versican. Axons did not regenerate into the lesion surround that contained astrocytes and abundant neurocan and versican. After transection and treatment with hyaluronidase, there was a significant increase in the number of cut dopaminergic nigral axons growing up to 800 microm anterior to the site of transection. However, cut dopaminergic nigral axons still did not regenerate into the lesion surround that contained reduced (albeit residual) neurocan and versican immunoreactivity. Thus, partial degradation of hyaluronan and chondroitin sulfate and depletion of hyaluronan-binding CSPGs enhances local sprouting of cut CNS axons, but long-distance regeneration fails in regions containing residual hyaluronan-binding CSPGs. Hyaluronan, chondroitin sulfate and hyaluronan-binding CSPGs therefore likely contribute toward the failure of spontaneous axon regeneration in the injured adult mammalian brain and spinal cord.
Moon
Lawrence D F
LD
Physiological Laboratory, University of Cambridge, Cambridge, United Kingdom. lmoon@miamiproject.med.miami.edu
Asher
Richard A
RA
Fawcett
James W
JW
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000502:physiology; D001369:Axons / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D019771:Axotomy / Q000379:methods; D002490:Central Nervous System / Q000166:cytology*; D019765:Chondroitin ABC Lyase / Q000378:metabolism; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism; D018960:Hyaluronan Receptors / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology*; D015151:Immunoblotting; D007150:Immunohistochemistry; D037181:Lectins, C-Type; D009416:Nerve Regeneration; D009419:Nerve Tissue Proteins / Q000378:metabolism; D058580:Neurocan; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D053675:Versicans
D011508:Chondroitin Sulfate Proteoglycans; D006025:Glycosaminoglycans; D018960:Hyaluronan Receptors; D037181:Lectins, C-Type; D009419:Nerve Tissue Proteins; D058580:Neurocan; C508322:VCAN protein, human; C508324:Vcan protein, rat; C038142:chondroitin sulfate glycosaminoglycan; D053675:Versicans; C076817:NCAN protein, human; D002809:Chondroitin Sulfates; D014446:Tyrosine 3-Monooxygenase; D006821:Hyaluronoglucosaminidase; D019765:Chondroitin ABC Lyase; D004298:Dopamine