Tools for targeted manipulation of the mouse genome.
In the postgenomic era the mouse will be central to the challenge of ascribing a function to the 40,000 or so genes that constitute our genome. In this review, we summarize some of the classic and modern approaches that have fueled the recent dramatic explosion in mouse genetics. Together with the sequencing of the mouse genome, these tools will have a profound effect on our ability to generate new and more accurate mouse models and thus provide a powerful insight into the function of human genes during the processes of both normal development and disease.
van der Weyden
Louise
L
The Wellcome Trust Sanger Institute, Hinxton, Cambs CB10 1SA, United Kingdom.
Adams
David J
DJ
Bradley
Allan
A
D000818:Animals; D002869:Chromosome Aberrations; D018390:Gene Targeting / Q000379:methods*; D016678:Genome; D019426:Integrases / Q000096:biosynthesis / Q000235:genetics; D051379:Mice / Q000235:genetics*; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D023421:Models, Animal; D016296:Mutagenesis; D011995:Recombination, Genetic; D015533:Transcriptional Activation; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases