Expression of heat-shock protein 25 immunoreactivity in the dental pulp and enamel organ during odontogenesis in the rat molar.
The present immunocytochemical study reports on the expression of heat-shock protein (Hsp) 25 during odontogenesis in rat molars from postnatal 1 to 100 days. Hsp 25 immunoreactivity (IR) appeared in the immature dental mesenchymal cells and the differentiating and differentiated odontoblasts. At 30 days, the coronal odontoblasts retained intense Hsp25-IR, whereas the odontoblasts in the root and floor pulp were initially weak or negative but increased in IR in the later stages, indicating that the expression of Hsp 25 reflects the differentiation status of odontoblasts. During amelogenesis, the secretory ameloblasts were Hsp 25 immunopositive and the enamel free area (EFA) cells showed intense Hsp 25-IR when they developed a ruffled border. Ruffle-ended ameloblasts (RA) also consistently showed intense Hsp 25-IR, but smooth ended ameloblasts (SA) showed weak IR. These data suggest that Hsp 25 is related to the formation and maintenance of the ruffled border of RA and EFA cells.
Ohshima
Hayato
H
Division of Oral Anatomy, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-dori, Niigata 951-8514, Japan. histoman@dent.niigata-u.ac.jp
Nakakura-Ohshima
Kuniko
K
Maeda
Takeyasu
T
D000375:Aging / Q000378:metabolism; D000565:Ameloblasts / Q000378:metabolism; D000566:Amelogenesis / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism; D002454:Cell Differentiation; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D004658:Enamel Organ / Q000378:metabolism*; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007150:Immunohistochemistry; D008963:Molar / Q000502:physiology*; D009363:Neoplasm Proteins / Q000378:metabolism*; D009804:Odontoblasts / Q000166:cytology / Q000378:metabolism; D009805:Odontogenesis / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C486114:Hspb1 protein, rat; D009363:Neoplasm Proteins