[Drug resistance and genotyping of Klebsiella pneumoniae in lower respiratory tract infection].
OBJECTIVE
To understand the current drug resistance of Klebsiella pneumoniae in lower respiratory tract infection and the prevalence trend of extended spectrum beta-lactamase (ESBLs)-producing Klebsiella pneumoniae.


METHODS
Drug resistance in 86 strains of Klebsiella pneumoniae and ESBLs-producing strains were genotyped by randomly amplified DNA polymorphisms (RAPD).


RESULTS
ESBLs-producing strains accounted for 20.9% of the 86 strains, and 83.3% of the ESBLs-producing strains were from respiratory ICU. The drug resistance rate of ESBLs positive bacteria was much higher than that of ESBLs negative bacteria. ESBLs positive bacteria were resistant to both the quinolones and a minoglylosides. Eighteen ESBLs-producing strains were divided into 12 types. 3 strains had the same profile (genotype A), and 5 strains had an identical profile (genotype B), but other strains had different profiles.


CONCLUSIONS
Chromosomal DNA analysis proved that certain types of ESBLs-producing Klebsiella pneumoniae were epidemic in respiratory ICU. RAPD was an economic, simple but reliable method in epidemic study.
Shi
Jinling
J
Department of Laboratory Medicine, Wuhan University People's Hospital, Wuhan 430060, China.
Li
Yan
Y
Li
Congrong
C
Cai
Xuan
X
Li
Hongxia
H
Peng
Shaohua
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D024881:Drug Resistance, Bacterial; D005260:Female; D005838:Genotype; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged; D019105:Random Amplified Polymorphic DNA Technique; D012141:Respiratory Tract Infections / Q000382:microbiology*; D001618:beta-Lactamases / Q000096:biosynthesis
D001618:beta-Lactamases