Platelet aggregation inhibitory activity of bovine, ovine, and caprine kappa-casein macropeptides and their tryptic hydrolysates.
Kappa-casein macropeptide (CMP) is one of the components of whey and is obtained as a by-product in cheesemaking. There has been increasing interest in research to find new uses of cheese industry by-products in order to improve their value and promote their use. Human platelet aggregation inhibitory activities of bovine, ovine, and caprine CMPs and their tryptic hydrolysates were studied. CMPs from the three species exhibited in vitro antithrombotic properties similar to the activity of the gamma-fibrinogen 400-411 peptide. Inhibitory activities increased following hydrolysis with trypsin. Active sequences were identified among the tryptic peptides by reversed-phase high-performance liquid chromatography with on-line mass spectrometry.
Manso
M A
MA
Instituto de Fermentaciones Industriales, Consejo Superior de Investigaciones Científicas, Juan de la Cierva 3, 28006 Madrid, Spain.
Escudero
C
C
Alijo
M
M
López-Fandiño
R
R
D000818:Animals; D002364:Caseins / Q000032:analysis*; D002417:Cattle; D002611:Cheese; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006041:Goats; D006868:Hydrolysis; D010446:Peptide Fragments / Q000032:analysis*; D010975:Platelet Aggregation Inhibitors / Q000032:analysis*; D012756:Sheep; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D002364:Caseins; D010446:Peptide Fragments; D010975:Platelet Aggregation Inhibitors