title: Lack of correlation between bronchial late allergic reaction to Dermatophagoides pteronyssinus and in vitro immunoglobulin E reactivity to histamine-releasing factor derived from mononuclear cells.
abstract: BACKGROUND
  Activity of immunoglobulin (Ig)E-dependent histamine-releasing factor (HRF) is dependent on the IgE molecules bound to the surface of basophils. Sera capable of passively sensitizing basophils to release histamine to HRF were designated IgE+ sera. IgE+ and HRF have been suggested to play a role in late allergic reaction (LAR).
  
  
  OBJECTIVE
  The working hypothesis was tested that IgE+ induces a LAR. Further, activity of HRF produced by mononuclear cells (HRF(mn)) was compared with that of recombinant HRF p23.
  
  
  METHODS
  Atopic patients (n = 82) were bronchially provoked with Dermatophagoides pteronyssinus extract and the change in forced expiratory volume in 1 second was monitored. A LAR was defined as forced expiratory volume in 1 second as percentage of baseline < 80% 4 to 10 hours after allergen challenge. The presence of HRF-responsive IgE in serum was determined using basophils sensitized in vitro by serum.
  
  
  RESULTS
  The presence of HRF(mn)-responsive IgE (IgE(mn+)) in serum was shown not be essential for a LAR: 63% of the patients with a LAR had no IgE(mn+) in their serum. Further, 71% of patients with IgE(mn+) did not have a LAR. HRF(mn) and recombinant HRF p23 were not equivalent in the bioassay: serum of 38 of 82 atopic patients sensitized basophils to release histamine to HRF(mn), whereas this was found with serum of 1 of 82 patients to HRF p23.
  
  
  CONCLUSIONS
  The results do not support the hypothesis that IgE(mn+) induces a LAR, but do not exclude the alternative hypothesis that HRFs are released during a LAR and contribute to asthma severity.
authors.affiliation: Department of Immunopathology, Sanquin Research at CLB, Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, The Netherlands.
authors.forename: Ilona Kleine
authors.initials: IK
authors.lastname: Budde
authors.forename: Christa E
authors.initials: CE
authors.lastname: Lopuhaa
authors.forename: Pleuni G
authors.initials: PG
authors.lastname: de Heer
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Langdon
authors.forename: Susan M
authors.initials: SM
authors.lastname: MacDonald
authors.forename: Jaring S
authors.initials: JS
authors.lastname: van der Zee
authors.forename: Rob C
authors.initials: RC
authors.lastname: Aalberse
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000009:adverse effects; D001249:Asthma / Q000097:blood / Q000276:immunology; D001491:Basophils / Q000276:immunology / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism / Q000494:pharmacology; D016535:Bronchial Hyperreactivity / Q000097:blood / Q000209:etiology*; D001985:Bronchial Provocation Tests; D005260:Female; D006636:Histamine Release; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000097:blood / Q000209:etiology* / Q000276:immunology; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D039981:Pyroglyphidae / Q000276:immunology*; D011852:Radioallergosorbent Test; D011994:Recombinant Proteins / Q000494:pharmacology; D012882:Skin Tests; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D039741:Antigens, Dermatophagoides; D014408:Biomarkers, Tumor; D011994:Recombinant Proteins; D000090324:Tumor Protein, Translationally-Controlled 1; D007073:Immunoglobulin E