title: Blood leptin homeostasis: sex-associated differences in circulating leptin levels in rats are independent of tissue leptin expression.
abstract: Circulating leptin levels are higher in women than in men. The aim of the study has been to determine in rats the putative existence of sex-associated differences in leptin expression in different adipose tissue depots (gonadal, retroperitoneal, mesenteric and inguinal white adipose tissue and interscapular brown adipose tissue) and the relationship with circulating leptin levels. Adult male and female Wistar rats acclimated to 22 degrees C or to 28 degrees C were used. Leptin mRNA expression was assessed by northern blot and serum leptin levels by enzyme-linked immunosorbent assay (ELISA). Contrary to what happens in humans, we report here that male rats acclimated to standard animal house conditions (22 degrees C) have a higher leptin concentration in blood than female rats. This situation cannot be explained by a greater size of the fat depots in males, because the adiposity index is similar in both genders, but are rather associated to higher leptin specific mRNA expression by the white adipose tissue. Around thermoneutral conditions (28 degrees C), sex related differences in leptin mRNA expression disappear, but the gender difference in circulating leptin levels remains. In addition, leptin mRNA expression is higher in both genders in thermoneutral conditions but this is not reflected in changes in the circulating leptin levels. In conclusion, this study shows that rat circulating leptin levels are finely regulated, and not exclusively dependent on leptin mRNA expression, but other mechanisms are also involved, possibly regarding leptin rate of degradation.
authors.affiliation: Departamento de Biología Fundamental y Ciencias de la Salud, Universitat de les Illes Balears, Cra. Valldemossa Km 7.5, E-07071, Palma de Mallorca, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Mulet
authors.forename: Catalina
authors.initials: C
authors.lastname: Picó
authors.forename: Paula
authors.initials: P
authors.lastname: Oliver
authors.forename: Andreu
authors.initials: A
authors.lastname: Palou
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000378:metabolism; D000818:Animals; D005260:Female; D006706:Homeostasis / Q000502:physiology*; D020738:Leptin / Q000097:blood* / Q000378:metabolism; D008297:Male; D009928:Organ Specificity; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012737:Sex Factors; D013696:Temperature
chemical_list: D020738:Leptin; D012333:RNA, Messenger