Passive transfer of serum antibodies induced by BBG2Na, a subunit vaccine, in the elderly protects SCID mouse lungs against respiratory syncytial virus challenge.
Respiratory syncytial virus (RSV) is responsible for severe low respiratory tract infections in young infants and the elderly. To investigate whether BBG2Na, a recombinant subunit vaccine comprising aa 130-230 of the RSV G protein, induced protective Abs in subjects over 60 years during phase II clinical trial, pre- and postimmunization sera of individuals immunized with BBG2Na or placebo were transferred into SCID mice before RSV challenge. These sera dose-dependently reduced lung RSV titers. However at some points of serial dilutions, postimmunization sera of BBG2Na-immunized subjects only were significantly more efficient than the corresponding preimmunization sera, in agreement with the induction of an increased Ab response against multiple epitopes on RSV-A G protein. Thus, BBG2Na is immunogenic in the elderly and confers passive protection in mice after serum transfer. To our knowledge, this is the first description of protective Abs induced by a subunit vaccine in human.
Plotnicky-Gilquin
Hélène
H
Centre d'Immunologie Pierre Fabre, 5 Av. Napoléon III, F74 164, Saint-Julien-en-Genevois, France. helene.plotnicky.gilquin@pierre-fabre.com
Cyblat-Chanal
Dominique
D
Goetsch
Liliane
L
Lacheny
Christine
C
Libon
Christine
C
Champion
Thierry
T
Beck
Alain
A
Pasche
Hélène
H
Nguyen
Thien N
TN
Bonnefoy
Jean Yves
JY
Bouveret-le-Cam
Nancy
N
Corvaïa
Nathalie
N
D000281:Administration, Intranasal; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000914:Antibodies, Viral / Q000008:administration & dosage* / Q000096:biosynthesis; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D015707:HN Protein / Q000235:genetics; D006801:Humans; D007116:Immunization, Passive; D008168:Lung / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D008875:Middle Aged; D011995:Recombination, Genetic; D018357:Respiratory Syncytial Virus Infections / Q000517:prevention & control* / Q000821:virology; D022261:Respiratory Syncytial Virus Vaccines / Q000008:administration & dosage / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology; D014759:Viral Envelope Proteins
D000914:Antibodies, Viral; D015707:HN Protein; D022261:Respiratory Syncytial Virus Vaccines; D022223:Vaccines, Subunit; D014759:Viral Envelope Proteins; C116204:attachment protein G