title: Depletion of intracellular zinc from neurons by use of an extracellular chelator in vivo and in vitro.
abstract: The membrane-impermeable chelator CaEDTA was introduced extracellularly among neurons in vivo and in vitro for the purpose of chelating extracellular Zn(2+). Unexpectedly, this treatment caused histochemically reactive Zn(2+) in intracellular compartments to drop rapidly. The same general result was seen with intravesicular Zn(2+), which fell after CaEDTA infusion into the lateral ventricle of the brain, with perikaryal Zn(2+) in Purkinje neurons (in vivo) and with cortical neurons (in vitro). These findings suggest either that the volume of zinc ion efflux and reuptake is higher than previously suspected or that EDTA can enter cells and vesicles. Caution is therefore warranted in attempting to manipulate extracellular or intracellular Zn(2+) selectively.
authors.affiliation: NeuroBioTex, Inc., Galveston, Texas 77550, USA. chris@neurobiotex.com
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Frederickson
authors.forename: Sang W
authors.initials: SW
authors.lastname: Suh
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Koh
authors.forename: Yoo K
authors.initials: YK
authors.lastname: Cha
authors.forename: Richard B
authors.initials: RB
authors.lastname: Thompson
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: LaBuda
authors.forename: Rengarajan V
authors.initials: RV
authors.lastname: Balaji
authors.forename: Math P
authors.initials: MP
authors.lastname: Cuajungco
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000648:ultrastructure; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology*; D003864:Depression, Chemical; D004492:Edetic Acid / Q000494:pharmacology*; D005110:Extracellular Space / Q000378:metabolism; D006651:Histocytochemistry; D007276:Injections, Intraventricular; D007424:Intracellular Fluid / Q000378:metabolism; D008297:Male; D008845:Microinjections; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009589:Nitrogen Oxides; D051381:Rats; D017207:Rats, Sprague-Dawley; D013096:Spermine / Q000031:analogs & derivatives* / Q000494:pharmacology; D015032:Zinc / Q000378:metabolism*
chemical_list: D002614:Chelating Agents; D020030:Nitric Oxide Donors; D009589:Nitrogen Oxides; C091861:spermine nitric oxide complex; D013096:Spermine; D004492:Edetic Acid; D015032:Zinc