A novel method for preparing histology slides without a microtome.
A study was undertaken to determine if it might be possible to prepare tissue sections on slides without the use of paraffin embedding, microtome sectioning, or cryostat sectioning which involve equipment and training not always available to scholars or professionals wishing to examine tissue microscopically. After evaluating many different reagents, cutting instruments and solid supports, we developed a method involving application of super glue to a slide, adhering a section of tissue to it, cutting the tissue with a disposable microtome blade, staining the tissue and removing the superglue with a commercially available product. The sections are similar to those sectioned on a microtome, but do not at this time equal their quality. However, histoarchitecture is preserved and individual cell morphology is usually good. We conclude that this is a viable method for preparing histology sections without the use of a microtome or cryostat, something long thought impossible. We have dubbed the method 'RAMP' (Rapid Adhesive-Mediated Procedure).
Troyer
D L
DL
Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA. troyer@vet.ksu.edu
Cash
W C
WC
Provo-Klimek
J
J
Kennedy
G A
GA
D000269:Adhesives; D008722:Methods; D008853:Microscopy; D008867:Microtomy; D010232:Paraffin; D013048:Specimen Handling / Q000379:methods*; D013194:Staining and Labeling
D000269:Adhesives; D010232:Paraffin