Determination of the diterpenoid, kaurenoic acid, in Annona glabra by HPLC.
The isolation of ent-kaur-16-en-19-oic acid (1) from the leaves and bark of Annona glabra and its determination by reversed-phase HPLC with a photodiode array detector is described. The results indicate that 1 accumulates in the bark of the plant with only very low concentrations in the leaves. Levels of 1 varied throughout the year being highest for bark and leaves in the autumn (5.29 +/- 0.23 and 0.27 +/- 0.01 mg/g dry weight, respectively) and lowest in the spring for bark and in the winter for leaves (4.00 +/- 0.25 and 0.11 +/- 0.01 mg/g dry weight, respectively).
Oliveira
Brás H
BH
UFPR, Departamento de Química, CP19081, 81531-970, Curitiba-PR, Brazil. bho@quimica.ufpr.br
Sant'Ana
Antônio Euzébio G
AE
Bastos
Denise Z L
DZ
D031041:Annona / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification; D045786:Diterpenes, Kaurane; D015394:Molecular Structure; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D012621:Seasons
D004224:Diterpenes; D045786:Diterpenes, Kaurane; D010936:Plant Extracts; C014341:argyrophilic acid; C012042:steviol; C051436:kaurenoic acid