[Associative analysis of the connection between mutation C1167T in the catalase gene and polymorphic marker D6S392 nearby the Mn-dependent superoxide dismutase gene with diabetic microangiopathies].
OBJECTIVE
To study allele polymorphism of two variable regions [C1167T substitution in the catalase (CAT) gene D6S392 microsatellite near the Mn-dependent superoxide dismutase (SOD2) gene] was studied in insulin-dependent diabetic (IDDM) patients with (n = 36) or without (n = 56) diabetic nephropathy, and with (n = 30) or without (n = 44) diabetic retinopathy.


METHODS
Both polymorphic regions were amplified using polymerase chain reaction (PCR). PCR products were separated using polyacrylamide (D6S366) or agarose (C1167T) gel electrophoresis. In a case of C1167, PCR-amplified products were digested with BstXI restriction endonuclease before electrophoresis. A significance of the difference between allele distributions in complicated and uncomplicated IDDM patients was estimated using the exact Fisher's test.


RESULTS
No significant difference was observed in allele and genotype frequencies in complicated and uncomplicated IDDM subjects.


CONCLUSIONS
C1167 polymorphism in the CAT gene and D6S366 near the SOD2 gene are not associated with the development of diabetic nephropathy and diabetic retinopathy in IDDM.
Chistiakov
D A
DA
Savost'ianov
K V
KV
Chugunova
L A
LA
Shamkhalova
M Sh
MSh
Shestakova
M V
MV
Milen'kaia
T M
TM
Nosikov
V V
VV
Dedov
I I
II
Debabov
V G
VG
D000293:Adolescent; D000328:Adult; D000483:Alleles; D002374:Catalase / Q000235:genetics*; D003928:Diabetic Nephropathies / Q000235:genetics*; D003930:Diabetic Retinopathy / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013482:Superoxide Dismutase / Q000235:genetics*
D002374:Catalase; D013482:Superoxide Dismutase