Red blood cell transfusions are associated with alterations in self-reactive antibody repertoires of plasma IgM and IgG, independent of the presence of a specific immune response toward RBC antigens.
Immunization to allogeneic RBC antigens occurs in transfused patients, and may be associated with the development of RBC-destructive antibodies directed against autologous RBC. The present study investigates the effect of transfusion of allogeneic RBC on self-reactive antibody repertoires, independent of the presence of a specific immune response directed toward RBC antigens. Antibody repertoires of IgM and IgG in plasma of hematological patients prior to starting a transfusion regime and of hematological patients receiving regular transfusions of leukocyte-depleted RBC were analyzed using quantitative immunoblotting on a panel of antigens derived from tissue and cell extracts, followed by multiparametric statistical treatment of the data. Multiparametric statistical analysis discriminated between self-reactive antibody repertoires of patients prior to starting a transfusion regime and those receiving regular RBC transfusions (IgM, 0.0001 < P < 0.0244/IgG, 0.0002 < P < 0.0088, depending on the tissue extract), whereas antibody repertoires of plasma IgM and IgG toward bacterial antigens were similar between groups of patients (P > 0.05). We conclude that the challenge of the immune system with allogeneic RBC antigens influences self-reactive antibody repertoires of plasma IgM and IgG, independent of the presence of a specific immune response toward RBC antigens.
Stahl
Dorothea
D
INSERM U430 and Université Pierre et Marie Curie, Hôpital Broussais, 75014 Paris, France. stahld@uni-muenster.de
Lacroix-Desmazes
Sébastien
S
Sibrowski
Walter
W
Kazatchkine
Michel D
MD
Kaveri
Srini V
SV
D000918:Antibody Specificity / Q000276:immunology; D000941:Antigens / Q000097:blood / Q000276:immunology; D019540:Area Under Curve; D001323:Autoantibodies / Q000096:biosynthesis / Q000276:immunology*; D015153:Blotting, Western; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D025341:Principal Component Analysis; D014184:Transplantation, Homologous / Q000276:immunology
D000941:Antigens; D001323:Autoantibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M