The cross-reactivity of I-A(g7) and I-A(d) tetramers.
OBJECTIVE
To investigate the cross-reactivity between glutamic acid decarboxylase (GAD)-I-A(g7) and I-A(d) tetramer in diabetes-prone non-obese diabetic (NOD) mice (I-A(g7)) and diabetes-free Balb/c mice (I-A(d)).


METHODS
Two GAD peptide I-A(g7) and I-A(d) tetramers were generated and compared for phenotype and function of sorted GAD peptide I-A(g7) and I-A(d) tetramer-positive (tet+) T cells.


RESULTS
The cross-reactivity is shown in either tetramer positive percentage or tetramer staining intensity. The NOD and Balb/c derived-tet+ T cells were able to be cross-stained by GAD peptide I-A(g7) and I-A(d) tetramers, and responded to both irradiated NOD and Balb/c splenotyes under stimulation by synthetic and recombinant GAD peptides.


CONCLUSIONS
Although I-A(g7) and I-A(d) are closely related in biochemical and biological aspects, their most notable difference is the presence or absence of a negatively charged residue at position beta57 that links to insulin-dependent diabetes mellitus.
Peng
Yong
Y
Department of Neurology, The Third Affiliated Hospital, Hunan Medical University, Changsha, Hunan 410013, China. yongpeng70@yahoo.com
Liu
Chih-pin
CP
D000595:Amino Acid Sequence; D000818:Animals; D003429:Cross Reactions; D005260:Female; D005968:Glutamate Decarboxylase / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000276:immunology
D000949:Histocompatibility Antigens Class II; C407099:I-A g7 antigen; C111389:I-Ad antigen; D005968:Glutamate Decarboxylase