title: Polymer-coated fibrous extraction medium for sample preparation coupled to microcolumn liquid-phase separations.
abstract: Polymer-coated fibrous material has been introduced as the extraction medium for a miniaturized sample preparation method being coupled with microcolumn liquid chromatography. The preconcentration and the subsequent liquid chromatographic separation of tricyclic antidepressants (TCAs) drugs, amitriptyline, imipramine, nortriptyline and desipramine, was carried out with the hyphenated system. Several basic experimental parameters, such as extraction and separation conditions, were investigated along with the applicability of the method for the analysis of biological fluids. The results clearly showed that the on-line coupled system could be a powerful tool for the analysis of complex mixtures in biological matrix without a large solvent consumption and specially designed instruments. The lowest limit of quantification was quite acceptable for the analysis of TCAs in clinical and forensic situations.
authors.affiliation: School of Materials Science, Toyohashi University of Technology, Toyohashi 441-8580, Japan.
authors.forename: Motohiro
authors.initials: M
authors.lastname: Imaizumi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Saito
authors.forename: Makiko
authors.initials: M
authors.lastname: Hayashida
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Takeichi
authors.forename: Hiroo
authors.initials: H
authors.lastname: Wada
authors.forename: Kiyokatsu
authors.initials: K
authors.lastname: Jinno
mesh_terms: D000929:Antidepressive Agents, Tricyclic / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010080:Oxazoles / Q000032:analysis / Q000737:chemistry; D011108:Polymers / Q000032:analysis* / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000379:methods*
chemical_list: D000929:Antidepressive Agents, Tricyclic; D010080:Oxazoles; D011108:Polymers; C450140:poly(4-phenylene-2,6-benzobisoxazole)