Characterization of human alpha-dystrobrevin isoforms in HL-60 human promyelocytic leukemia cells undergoing granulocytic differentiation.
The biochemical properties and spatial localization of the protein alpha-dystrobrevin and other isoforms were investigated in cells of the human promyelocytic leukemia line HL-60 granulocytic differentiation as induced by retinoic acid (RA). Alpha-dystrobrevin was detected both in the cytosol and the nuclei of these cells, and a short isoform (gamma-dystrobrevin) was modified by tyrosine phosphorylation soon after the onset of the RA-triggered differentiation. Varying patterns of distribution of alpha-dystrobrevin and its isoforms could be discerned in HL-60 promyelocytes, RA-differentiated mature granulocytes, and human neutrophils. Moreover, the gamma-dystrobrevin isoform was found in association with actin and myosin light chain. The results provide new information about potential involvement of alpha-dystrobrevin and its splice isoforms in signal transduction in myeloid cells during induction of granulocytic differentiation and/or at the commitment stage of differentiation or phagocytic cells.
Kulyte
Agné
A
Division of Medical Microbiology, Linköpings Universitet, SE-581 85 Linköping, Sweden. agne.kulyte@cgb.ki.se
Navakauskiene
Ruta
R
Treigyte
Grazina
G
Gineitis
Arunas
A
Bergman
Tomas
T
Magnusson
Karl-Eric
KE
D000199:Actins / Q000378:metabolism; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000737:chemistry* / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D049029:Dystrophin-Associated Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D006098:Granulocytes / Q000166:cytology* / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D015151:Immunoblotting; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D018994:Myosin Light Chains / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D010587:Phagocytosis; D010766:Phosphorylation; D011233:Precipitin Tests; D020033:Protein Isoforms; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism
D000199:Actins; D003598:Cytoskeletal Proteins; D049029:Dystrophin-Associated Proteins; D008565:Membrane Proteins; D018994:Myosin Light Chains; D020033:Protein Isoforms; C098885:dystrobrevin; D014443:Tyrosine; D014357:Trypsin