A scalable high-throughput chemical synthesizer.
A machine that employs a novel reagent delivery technique for biomolecular synthesis has been developed. This machine separates the addressing of individual synthesis sites from the actual process of reagent delivery by using masks placed over the sites. Because of this separation, this machine is both cost-effective and scalable, and thus the time required to synthesize 384 or 1536 unique biomolecules is very nearly the same. Importantly, the mask design allows scaling of the number of synthesis sites without the addition of new valving. Physical and biological comparisons between DNA made on a commercially available synthesizer and this unit show that it produces DNA of similar quality.
Livesay
Eric A
EA
Center for Biomedical Inventions (CBI), University of Texas Southwestern Medical Center, Dallas, Texas 75390-9187, USA. Eric.Livesay@UTSouthwestern.edu
Liu
Ying-Horng
YH
Luebke
Kevin J
KJ
Irick
Joel
J
Belosludtsev
Yuri
Y
Rayner
Simon
S
Balog
Robert
R
Johnston
Stephen Albert
SA
D004247:DNA / Q000096:biosynthesis / Q000138:chemical synthesis* / Q000191:economics; D019020:Directed Molecular Evolution / Q000191:economics / Q000295:instrumentation* / Q000379:methods*; D007202:Indicators and Reagents; D015345:Oligonucleotide Probes / Q000096:biosynthesis / Q000138:chemical synthesis / Q000191:economics; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000191:economics / Q000295:instrumentation / Q000379:methods; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D007202:Indicators and Reagents; D015345:Oligonucleotide Probes; D004247:DNA