A possible purification method of DNAs' fragments from humic matters in soil extracts using novel stimulus responsive polymer adsorbent.
Novel stimulus responsive, uniformly sized polymer-based adsorbent was prepared for a possible purification method of DNAs from humic matters in soil extracts. The prepared polymer adsorbent has a pair of anion exchangeable and cation exchangeable polymeric selectors, which are reversibly responded by the changes of column temperature as well as pH of mobile phase. At pH 5, DNAs and humic matters were completely adsorbed on the polymer adsorbent at room temperature, while up to 90% of the adsorbed DNAs were released and recovered at 70 degrees C with no release of adsorbed humic matters. Cleaning up of the polymer adsorbent could be performed by washing the adsorbed humic matters with alkaline mobile phase (pH 9) to recover those and realize repeatable use of the adsorbent.
Hosoya
Ken
K
Department of Polymer Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. kenpc@ipc.kit.ac.jp
Kubo
Takuya
T
Tanaka
Nobuo
N
Haginaka
Jun
J
D000327:Adsorption; D004247:DNA / Q000302:isolation & purification*; D006812:Humic Substances / Q000032:analysis*; D011108:Polymers / Q000032:analysis*; D012987:Soil / Q000032:analysis*; D013696:Temperature
D006812:Humic Substances; D011108:Polymers; D012987:Soil; D004247:DNA