[Effect of acrosin inhibitor KF-950 on acrosin activity and acrosome of human sperm].
OBJECTIVE
To study the inhibitory effect of KF-950 on human acrosin and sperm acrosome.


METHODS
Human acrosin was extracted and purified with 2% acetic acid, and its residual activity was evaluated by BAEE/ADH assay after treated with different concentrations of KF-950. ABC assay was used to observe the effect of KF-950 on human acrosome with Biotin-PSA as a probe.


RESULTS
1. The activity of normal sperm acrosin was (37.65 +/- 4.47) U/L. 2. The residual activity was inversely related to the concentration of KF-950 (r = -0.998), and had a dose-response curve. The result could be described by Y = 7.57-1.895X. 3. With increase of KF-950 concentration and prolongation of action time, the staining rate of acrosome obviously dropped (P < 0.01).


CONCLUSIONS
KF-950 directly inhibits acrosin activity and assumely injures sperm acrosome. It might be a new kind of highly effective inhibitor.
Zhan
Ying
Y
Department of Obstetric and Gynecology, 401st Hospital of PLA, Qingdao, Shandong 266071, China.
Zhao
Ya-Nan
YN
Li
Zong-Lin
ZL
Li
Li
L
Song
Qian
Q
D000176:Acrosin / Q000037:antagonists & inhibitors*; D000177:Acrosome / Q000378:metabolism*; D006801:Humans; D008297:Male; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology
D000176:Acrosin