Neural cell adhesion molecule-mediated neurite outgrowth is repressed by overexpression of HES-1.
The neural cell adhesion molecule (NCAM) stimulates neurite outgrowth by activating intracellular signaling cascades. We investigated the role of the transcriptional repressor HES-1 in NCAM-dependent neurite outgrowth by estimating neurite extension from PC12-E2 cells grown in coculture with NCAM-negative or NCAM-positive fibroblasts. PC12-E2 cells were transiently transfected with an expression plasmid encoding HES-1. We found that expression of HES-1 inhibited NCAM-dependent neurite outgrowth. Treatment with arachidonic acid (an important messenger in NCAM-dependent signaling) restored NCAM-induced neurite outgrowth inhibited by HES-1. These results suggest that HES-1 is a regulator of intracellular signal transduction stimulated by cell adhesion molecules involved in neurite outgrowth.
Jessen
Ulla
U
Protein Laboratory, Institute of Molecular Pathology, University of Copenhagen, Copenhagen, Denmark.
Novitskaya
Vera
V
Walmod
Peter S
PS
Berezin
Vladimir
V
Bock
Elisabeth
E
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins; D018920:Coculture Techniques / Q000379:methods; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D007150:Immunohistochemistry; D007739:L Cells; D051379:Mice; D009414:Nerve Growth Factors / Q000378:metabolism; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism*; D016501:Neurites / Q000502:physiology*; D016716:PC12 Cells; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D000072056:Transcription Factor HES-1; D014158:Transcription, Genetic; D014162:Transfection
D016718:Arachidonic Acid; D051792:Basic Helix-Loop-Helix Proteins; D018398:Homeodomain Proteins; D009414:Nerve Growth Factors; D019006:Neural Cell Adhesion Molecules; D011994:Recombinant Proteins; D000072056:Transcription Factor HES-1; C489734:Hes1 protein, mouse; C489735:Hes1 protein, rat