Urinary free light chain analysis by the Freelite immunoassay: a preliminary study in multiple myeloma.
Evaluate the Freelite free light chain immunoassay for urine analysis in myeloma. Urine specimens from 20 patients were analyzed by Freelite (The Binding Site) and SDS-agarose gel electrophoresis (Hydragel protéinurie, Sebia). Using the kappa/lambda ratio, Freelite was more sensitive than electrophoresis to detect free light chains, but concentration was overestimated in 75% of cases. Despite high sensitivity and full automation, Freelite inaccurately measures monoclonal free light chains in urine.
Le Bricon
Thierry
T
Laboratoire de Biochimie A, Hôpital St-Louis AP-HP, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France. thierry.le-bricon@sls.ap-hop-paris.fr
Bengoufa
Djaouida
D
Benlakehal
Mourad
M
Bousquet
Bernard
B
Erlich
Danielle
D
D000704:Analysis of Variance; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007147:Immunoglobulin Light Chains / Q000652:urine*; D008297:Male; D009101:Multiple Myeloma / Q000652:urine*; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D012680:Sensitivity and Specificity
D007147:Immunoglobulin Light Chains; D011933:Reagent Kits, Diagnostic