Kinetic study of delta-Ala induced porphyrins in mice using photoacoustic and fluorescence spectroscopies.
The production of delta-aminolevulinic acid (ALA)-induced porphyrins in mice skin and blood was studied by photoacoustic and fluorescence spectroscopies. Mice were intraperitoneally administered with 30 mg/kg of ALA. The abdominal skin was subsequently excised at specific times within an 8-h interval and its absorption spectrum obtained by photoacoustics. The highest porphyrins concentration in skin, determined from the optical absorption of the Soret band at 410 nm, was found to occur nearly 2 h after ALA administration, but a first peak was also observed at approximately 15 min. Our hypothesis that the first peak represents the porphyrins content in blood vessels within the skin, whereas the second peak corresponds to porphyrins production in skin tissue, was confirmed by analysing the evolution of protoporphyrin IX content in plasma extracted intracardiacally. By finally applying phase resolved photoacoustic spectroscopy, we were able to evaluate the mean depth at which porphyrins are generated.
Stolik
Suren
S
Departamento de Física, Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear-CITMA, Calle 30 No. 502, Miramar, Playa, Ciudad de la Habana, Cuba. stolik@fis.cinvestav.mx
Tomás
Sergio A
SA
Ramón-Gallegos
Eva
E
Sánchez
Feliciano
F
D000622:Aminolevulinic Acid / Q000494:pharmacology*; D000818:Animals; D005260:Female; D007700:Kinetics; D008543:Melanins / Q000378:metabolism; D051379:Mice; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011166:Porphyrins / Q000378:metabolism; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
D008543:Melanins; D017319:Photosensitizing Agents; D011166:Porphyrins; D000622:Aminolevulinic Acid