title: [Frequency of the 185delAG mutation in the BRCA1 gene in Chilean healthy women with family history of breast cancer].
abstract: BACKGROUND
  Breast cancer is the most common malignancy among women, and is the second cause of cancer mortality among Chilean women. Female mortality due to breast cancer in Chile has shown a steady increase from 9.5 deaths per 100.000 women in 1985 to 12.8 deaths per 100.000 in 1995. A family history of breast cancer is one of the main risk factors for the development of the disease. BRCA1 and BRCA2 are two major hereditary breast cancer susceptibility genes. Mutations in these genes are associated to inherited breast cancer; 664 predisposing mutations have been described, but in specific populations only some of them, such as 185delAG have been found to be associated with susceptibility to breast cancer.
  
  
  OBJECTIVE
  To establish the frequency of the 185delAG mutation in the BRCA1 gene in Chilean healthy women with a family history of breast cancer.
  
  
  METHODS
  The 185delAG mutation was studied by mismatch polymerase chain (PCR) reaction in 382 Chilean healthy women with at least two relatives affected with breast cancer. The PCR products were digested with the restriction enzyme HinfI. Digestion of the normal allele (170 pb fragment) produces a 150 pb fragment; the PCR product for the mutant allele does not contain a site for HinfI and therefore remains as a 170 bp fragment after digestion.
  
  
  RESULTS
  One of the 382 healthy women presented the fragment of 170 pb after digestion with HinfI suggesting that she was heterozygous carrier for this mutation. The mutant patient had a mammography without suspicion of cancer.
  
  
  CONCLUSIONS
  The frequency of the 185delAG mutation in BRCA1 was 0.26% (1/382) in Chilean healthy women with a family history of breast cancer.
authors.affiliation: Programa de Genética Humana, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile. ljara@machi.med.uchile.cl
authors.forename: Lilian
authors.initials: L
authors.lastname: Jara
authors.forename: Sandra
authors.initials: S
authors.lastname: Ampuero
authors.forename: Lorena
authors.initials: L
authors.lastname: Seccia
authors.forename: Mario
authors.initials: M
authors.lastname: Bustamante
authors.forename: Rafael
authors.initials: R
authors.lastname: Blanco
authors.forename: Eudocia
authors.initials: E
authors.lastname: Santibáñez
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Reyes
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Ojeda
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000235:genetics*; D002677:Chile; D004587:Electrophoresis, Agar Gel; D005260:Female; D017353:Gene Deletion; D019398:Genes, BRCA1; D006801:Humans; D017403:In Situ Hybridization; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D010375:Pedigree; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012307:Risk Factors; D017422:Sequence Analysis, DNA