Extended conformation of mammalian translation elongation factor 1A in solution.
The conformation of mammalian elongation factor eEF1A in solution was examined by the small angle neutron scattering and scanning microcalorimetry. We have found that in contrast to the bacterial analogue the eEF1A molecule has no fixed rigid structure in solution. The radius of gyration of the eEF1A molecule (5.2 nm) is much greater than that of prokaryotic EF1A. The specific heat of denaturation is considerably lower for eEF1A than for EF1A, suggesting that the eEF1A conformation is significantly more disordered. Despite its flexible conformation, eEF1A is found to be highly active in different functional tests. According to the neutron scattering data, eEF1A becomes much more compact in the complex with uncharged tRNA. The absence of a rigid structure and the possibility of large conformational change upon interaction with a partner molecule could be important for eEF1A functioning in channeled protein synthesis and/or for the well-known capability of the protein to interact with different ligands besides the translational components.
Budkevich
T V
TV
Institute of Molecular Biology and Genetics, National Academy of Sciences, 150 Zabolotnogo Street, Kiev, 03143 Ukraine.
Timchenko
A A
AA
Tiktopulo
E I
EI
Negrutskii
B S
BS
Shalak
V F
VF
Petrushenko
Z M
ZM
Aksenov
V L
VL
Willumeit
R
R
Kohlbrecher
J
J
Serdyuk
I N
IN
El'skaya
A V
AV
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000502:physiology; D002151:Calorimetry / Q000379:methods; D006153:Guanosine Diphosphate / Q000737:chemistry; D009502:Neutrons; D010452:Peptide Biosynthesis; D020648:Peptide Elongation Factor 1 / Q000031:analogs & derivatives / Q000737:chemistry* / Q000502:physiology; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D012346:RNA, Transfer, Amino Acyl / Q000737:chemistry; D011817:Rabbits; D012542:Scattering, Radiation; D012996:Solutions; D016963:Thermus thermophilus
D001426:Bacterial Proteins; D020648:Peptide Elongation Factor 1; D012346:RNA, Transfer, Amino Acyl; D012996:Solutions; D006153:Guanosine Diphosphate