Identification of differentially expressed genes in human salivary gland tumors by DNA microarrays.
Differentially expressed genes among different benign and malignant salivary gland tumors were identified by use of cDNA microarrays containing 19,000 human expressed sequence tags. Tumors were classified by using a subset of 486 genes. Benign Warthin's tumor and pleomorphic adenoma showed very distinctive gene expression patterns. One hundred and thirty-three genes differentiated the single malignant clear cell carcinoma from non-tumor salivary glands (P < 0.01), whereas only 16 genes separated it from the highly related benign pleomorphic adenoma (P < 0.01). Fifty-seven cDNAs were associated with mucoepidermoid carcinoma (P < 0.01). The identified genes might help to disclose the molecular mechanisms and processes underlying malignant salivary gland tumors.
Francioso
Francesca
F
Laboratorio di Genomica Funzionale, Sezione di Istologia ed Embriologia, Dipartimento di Morfologia ed Embriologia, Università degli Studi di Ferrara, 44100 Ferrara, Italy.
Carinci
Francesco
F
Tosi
Lara
L
Scapoli
Luca
L
Pezzetti
Furio
F
Passerella
Emanuele
E
Evangelisti
Rita
R
Pastore
Antonio
A
Pelucchi
Stefano
S
Piattelli
Adriano
A
Rubini
Corrado
C
Fioroni
Massimiliano
M
Carinci
Paolo
P
Volinia
Stefano
S
D018076:DNA, Complementary / Q000378:metabolism; D020224:Expressed Sequence Tags; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000235:genetics* / Q000378:metabolism*
D018076:DNA, Complementary; D012313:RNA