Recombinant human peroxiredoxin VI: preparation and protective properties in vitro.
cDNA of human peroxiredoxin VI, one of the recently discovered novel antioxidant proteins, was expressed in Escherichia coli cells. The expression product was obtained in water-soluble form and purified by a two-step chromatographic procedure using DEAE-Sepharose and Sephacryl S-200. According to CD data, the polypeptide chain of the recombinant human peroxiredoxin VI contains approximately 40% alpha-helical region and 30% beta-structure, which is the same as for native rat peroxiredoxin VI. The protective properties of the recombinant protein determined as its ability to prevent the inactivation of glutamine synthetase from E. coli in a model oxidation system were comparable with the protective properties of native rat peroxiredoxin VI.
Merkulova
M I
MI
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 177997, Russia. mmer@mail.ibch.ru
Shuvaeva
T M
TM
Radchenko
V V
VV
Yanin
B A
BA
Bondar
A A
AA
Sofin
A D
AD
Lipkin
V M
VM
D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D002852:Chromatography, Ion Exchange / Q000379:methods; D002942:Circular Dichroism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D005974:Glutamate-Ammonia Ligase / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology*; D054465:Peroxiredoxin VI; D054464:Peroxiredoxins; D017433:Protein Structure, Secondary; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology
D000975:Antioxidants; D018076:DNA, Complementary; D011994:Recombinant Proteins; D010544:Peroxidases; D054465:Peroxiredoxin VI; D054464:Peroxiredoxins; C515984:Prdx6 protein, rat; D005974:Glutamate-Ammonia Ligase