title: A simplified method for the detection of Y chromosome microdeletions in infertile men using a multiplex sequence-tagged site-based amplification.
abstract: Sixteen sequence-tagged sites (STSs) were combined in five amplification reactions, to screen for deletions of DNA fragments located within the AZFa, AZFb, and AZFc regions of the Y chromosome. This multiplex strategy is fast and reliable, and most of the azoospermia-associated deletions reported so far are detected with this simplified method. Internal control STSs are included that allow discrimination between deletion and failure of amplification.
authors.affiliation: Laboratoire de Biochimie et Biologie Moléculaire, Hôpital R Poincaré, 92380 Garches, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Charpenel
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Guillon
authors.forename: Olivier
authors.initials: O
authors.lastname: Dorson
authors.forename: Catherine
authors.initials: C
authors.lastname: Boucly
authors.forename: Bertille
authors.initials: B
authors.lastname: Mathieu
authors.forename: Martine
authors.initials: M
authors.lastname: Montagnon
authors.forename: Françoise
authors.initials: F
authors.lastname: Merlet
authors.forename: Geneviève
authors.initials: G
authors.lastname: Van Amerongen
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Bisson
authors.forename: Philippe
authors.initials: P
authors.lastname: de Mazancourt
mesh_terms: D001483:Base Sequence; D041322:Chromosomes, Human, Y; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D017384:Sequence Deletion; D016324:Sequence Tagged Sites