Systemic movement of a movement-deficient strain of Cucumber mosaic virus in zucchini squash is facilitated by a cucurbit-infecting potyvirus.
Zucchini squash (Cucurbita pepo) is a systemic host for most strains of the cucumovirus Cucumber mosaic virus (CMV), although the long-distance movement of the M strain of CMV (M-CMV) is inhibited in some cultivars. However, co-infection of zucchini plants with M-CMV and the potyvirus Zucchini yellow mosaic virus strain A (ZYMV-A) allowed M-CMV to move systemically, as demonstrated by tissue-print analysis. These doubly infected plants exhibited severe synergism in pathology. Infection of zucchini squash by M-CMV and an attenuated strain of ZYMV (ZYMV-AG) showed a milder synergy in pathology, in which ZYMV-AG also facilitated the long-distance movement of M-CMV similar to that promoted by ZYMV-A. Variation in the extent of synergy in pathology by the two strains of ZYMV did not correlate with differences in levels of accumulation of either virus. Thus, the extent of synergy in pathology is at least in part independent of the resistance-neutralizing function of the potyvirus.
Choi
Seung Kook
SK
Graduate School of Biotechnology, Korea University, Seoul, 136-701, Korea1.
Yoon
Ju Yeon
JY
Plant Virus GenBank, Department of Horticultural Science, Seoul Women's University, Seoul, 139-774, Korea2.
Ryu
Ki Hyun
KH
Plant Virus GenBank, Department of Horticultural Science, Seoul Women's University, Seoul, 139-774, Korea2.
Choi
Jang Kyung
JK
Division of Biological Environment, Kangwon National University, Chunchon 200-701, Korea3.
Palukaitis
Peter
P
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK4.
Park
Won Mok
WM
Graduate School of Biotechnology, Korea University, Seoul, 136-701, Korea1.
D017799:Cucumovirus / Q000472:pathogenicity* / Q000502:physiology*; D009068:Movement; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D017800:Potyvirus / Q000472:pathogenicity* / Q000502:physiology; D014675:Vegetables / Q000821:virology*