title: Urinary free light chain analysis by the Freelite immunoassay: a preliminary study in multiple myeloma.
abstract: Evaluate the Freelite free light chain immunoassay for urine analysis in myeloma. Urine specimens from 20 patients were analyzed by Freelite (The Binding Site) and SDS-agarose gel electrophoresis (Hydragel protéinurie, Sebia). Using the kappa/lambda ratio, Freelite was more sensitive than electrophoresis to detect free light chains, but concentration was overestimated in 75% of cases. Despite high sensitivity and full automation, Freelite inaccurately measures monoclonal free light chains in urine.
authors.affiliation: Laboratoire de Biochimie A, Hôpital St-Louis AP-HP, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France. thierry.le-bricon@sls.ap-hop-paris.fr
authors.forename: Thierry
authors.initials: T
authors.lastname: Le Bricon
authors.forename: Djaouida
authors.initials: D
authors.lastname: Bengoufa
authors.forename: Mourad
authors.initials: M
authors.lastname: Benlakehal
authors.forename: Bernard
authors.initials: B
authors.lastname: Bousquet
authors.forename: Danielle
authors.initials: D
authors.lastname: Erlich
mesh_terms: D000704:Analysis of Variance; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007147:Immunoglobulin Light Chains / Q000652:urine*; D008297:Male; D009101:Multiple Myeloma / Q000652:urine*; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D012680:Sensitivity and Specificity
chemical_list: D007147:Immunoglobulin Light Chains; D011933:Reagent Kits, Diagnostic