title: Structure of isolated nucleocapsids from venezuelan equine encephalitis virus and implications for assembly and disassembly of enveloped virus.
abstract: Venezuelan equine encephalitis virus (VEEV) is an important human and equine pathogen in the Americas, with widespread reoccurring epidemics extending from South America to the southern United States. Most troubling, VEEV has been made into a weapon by several countries and is currently restricted by the Centers for Disease Control and Prevention as a potential biological warfare and terrorism agent. To facilitate the development of antiviral compounds, the structure of the nucleocapsid isolated from VEEV has been determined by electron cryomicroscopy and image reconstruction and represents the first three-dimensional structure of a nucleocapsid isolated from a single-stranded enveloped RNA virus. The isolated VEEV nucleocapsid undergoes significant reorganization relative to its structure within VEEV. However, the isolated nucleocapsid clearly exhibits T=4 icosahedral symmetry, and its characteristic nucleocapsid hexons and pentons are preserved. The diameter of the isolated nucleocapsid is approximately 11.5% larger than that of the nucleocapsid within VEEV, with radial expansion being greatest near the hexons. Significantly, this is the first direct structural evidence showing that a simple enveloped virus undergoes large conformational changes during maturation, suggesting that the lipid bilayer and the transmembrane proteins of simple enveloped viruses provide the energy necessary to reorganize the nucleocapsid during maturation.
authors.affiliation: National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: Angel
authors.initials: A
authors.lastname: Paredes
authors.forename: Kathy
authors.initials: K
authors.lastname: Alwell-Warda
authors.forename: Scott C
authors.initials: SC
authors.lastname: Weaver
authors.forename: Wah
authors.initials: W
authors.lastname: Chiu
authors.forename: Stanley J
authors.initials: SJ
authors.lastname: Watowich
mesh_terms: D004666:Encephalitis Virus, Venezuelan Equine / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D008051:Lipid Bilayers; D008565:Membrane Proteins / Q000502:physiology; D019251:Nucleocapsid / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D019065:Virus Assembly
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins