Dorsal bipedicled island skin flap: a new flap model in mice.
Mice are popular animals for biomedical studies, but few skin flap models have been reported in them. To investigate the ischaemia/reperfusion phenomenon in skin flaps, we first investigated the vascular anatomy of murine dorsal skin and then designed a suitable murine dorsal skin flap model. In 120 mice, six distinct vascular patterns were identified, one being seen in 111 mice (93%). Based on this finding, in Part 2 of the study, 15 mice had flaps (4 x 4 cm) raised based on the two caudal vascular pedicles of the left and right deep circumflex iliac vessels as a bipedicled flap in which the mean (SD) survival was 96 (5)%. In a further 10 mice, flaps were raised based on a single pedicle, the left deep circumflex iliac vessel, as a monopedicled flap, in which the mean (SD) survival was 71 (12)%. The bipedicled flap model was then used to study ischaemia/reperfusion injury. Twenty flaps were subjected to eight hours of ischaemia and subsequent reperfusion, and their mean (SD) survival was 43 (26)%. Histological assessments were also carried out using neutrophil and leucocyte counts, and significant differences between groups were observed.
Minh
Trinh Cao
TC
Department of Plastic and Reconstructive Surgery, Biomedical Engineering, University of Tokyo, Japan.
Ichioka
Shigeru
S
Harii
Kiyonori
K
Shibata
Masahiro
M
Ando
Joji
J
Nakatsuka
Takashi
T
D000704:Analysis of Variance; D000818:Animals; D006085:Graft Survival / Q000502:physiology*; D008214:Lymphocytes / Q000502:physiology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008833:Microcirculation; D023421:Models, Animal; D009336:Necrosis; D009504:Neutrophils / Q000502:physiology; D015427:Reperfusion Injury / Q000503:physiopathology*; D015203:Reproducibility of Results; D012867:Skin / Q000098:blood supply / Q000503:physiopathology*; D013524:Surgical Flaps / Q000098:blood supply / Q000502:physiology*