title: Strawberry pectin methylesterase (PME): purification, characterization, thermal and high-pressure inactivation.
abstract: Pectin methylesterase (PME) was extracted from strawberries (Fragaria ananassa, cv Elsanta) and purified by affinity chromatography on a CNBr-Sepharose 4B-PME-inhibitor column. A single protein and PME activity peak was obtained. A biochemical characterization in terms of molecular mass, pI, and kinetic parameters of strawberry PME was performed. In a second step, the thermal and high-pressure stability of the enzyme was studied. Isothermal and combined isothermal-isobaric inactivation of purified strawberry PME could be described by a fractional-conversion model. Purified strawberry PME is much more stable toward high-pressure treatments in comparison to those from oranges and bananas.
authors.affiliation: Laboratory of Food Technology, Department of Food and Microbial Technology, Faculty of Agricultural and Applied Biological Sciences, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, B-3001 Leuven, Belgium.
authors.forename: Binh
authors.initials: B
authors.lastname: Ly-Nguyen
authors.forename: Ann M
authors.initials: AM
authors.lastname: Van Loey
authors.forename: Diana
authors.initials: D
authors.lastname: Fachin
authors.forename: Isabel
authors.initials: I
authors.lastname: Verlent
authors.forename: Thomas
authors.initials: T
authors.lastname: Duvetter
authors.forename: Son T
authors.initials: ST
authors.lastname: Vu
authors.forename: Chantal
authors.initials: C
authors.lastname: Smout
authors.forename: Marc E
authors.initials: ME
authors.lastname: Hendrickx
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D004795:Enzyme Stability; D031985:Fragaria / Q000201:enzymology; D007700:Kinetics; D011312:Pressure; D011489:Protein Denaturation; D013696:Temperature
chemical_list: D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase