title: Determination of nitrated polycyclic aromatic hydrocarbons and their precursors in biotic matrices.
abstract: Analytical method for the determination of ultra-trace levels of nitro-PAHs in various biotic matrices has been developed. Soxhlet extraction and/or solvent extraction enhanced by sonication were used for isolation of target analytes; GPC followed by SPE were employed for purification of crude extracts. GC-MS/NCI technique was utilised for identification/quantitation of target analytes. Performance characteristics of implemented method were obtained through thorough in-house validation procedure. The main sources of uncertainties were critically evaluated, possible strategies of their elimination/minimisation were considered and consequently employed. Examination of real-life samples of various foodstuffs (complete human diet, mate tea, pumpkin seed oil, parsley, sausages) was performed in this study.
authors.affiliation: Department of Food Chemistry and Analysis, Institute of Chemical Technology, Technica 3, 166 28 Prague 6, Czech Republic.
authors.forename: Bohuslav
authors.initials: B
authors.lastname: Dusek
authors.forename: Jana
authors.initials: J
authors.lastname: Hajslová
authors.forename: Vladimír
authors.initials: V
authors.lastname: Kocourek
mesh_terms: D002850:Chromatography, Gel; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D009566:Nitrates / Q000737:chemistry*; D011083:Polycyclic Compounds / Q000032:analysis*; D014835:Volatilization
chemical_list: D009566:Nitrates; D011083:Polycyclic Compounds