title: Bioavailability of Petit-Suisse cheese as food vehicle for iron fortification estimated by the prophylactic method.
abstract: Microencapsulated ferrous sulfate (SFE-171) and ferric orthophosphate in Petit-Suisse cheese were examined for iron bioavailability by the prophylactic method. The iron sources were industrially added to different samples of Petit-Suisse cheese, which were mixed with other food components in our laboratory before use. A reference standard diet inclusive of nonmicroencapsulated ferrous sulfate and a control diet low in iron content were prepared in the laboratory. The final iron content in the fortified diets was approximately 15 mg Fe/kg diet. These diets were administered to weaning rats for 23 days. The iron bioavailability was evaluated as the ratio of iron incorporated into hemoglobin to oral iron intake, thereby being estimated as 62.6 +/- 8.8% for ferrous sulfate and 59.2 +/- 10.6% for SFE-171, which were significantly effective at p < 0.01 compared to 43.4 +/- 10.5% for ferric orthophosphate. It thus turned out that SFE-171 was stable through industrial processing with Petit-Suisse cheese as the food vehicle and served as an iron fortifier equal to ferrous sulfate in bioavailability.
authors.affiliation: Radioisotope Laboratory, School of Pharmacy and Biochemistry, University of Buenos Aires, Junín 956, 1113-Buenos Aires, Argentina. aelysion@huemul.ffyb.uba.ar
authors.forename: Alexis E
authors.initials: AE
authors.lastname: Lysionek
authors.forename: Marcela B
authors.initials: MB
authors.lastname: Zubillaga
authors.forename: María J
authors.initials: MJ
authors.lastname: Salgueiro
authors.forename: Ricardo A
authors.initials: RA
authors.lastname: Caro
authors.forename: Moti
authors.initials: M
authors.lastname: Segal
authors.forename: Nava
authors.initials: N
authors.lastname: Shafran
authors.forename: Niva
authors.initials: N
authors.lastname: Shapira
authors.forename: José R
authors.initials: JR
authors.lastname: Boccio
mesh_terms: D000284:Administration, Oral; D018798:Anemia, Iron-Deficiency / Q000517:prevention & control*; D000818:Animals; D001682:Biological Availability; D002611:Cheese / Q000032:analysis; D004339:Drug Compounding; D005260:Female; D005290:Ferric Compounds / Q000493:pharmacokinetics; D005296:Ferrous Compounds / Q000493:pharmacokinetics; D005527:Food, Fortified; D006454:Hemoglobins / Q000737:chemistry*; D019266:Iron, Dietary / Q000493:pharmacokinetics*; D010713:Phosphatidylcholines / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014886:Weaning
chemical_list: D005290:Ferric Compounds; D005296:Ferrous Compounds; D006454:Hemoglobins; D019266:Iron, Dietary; D010713:Phosphatidylcholines; C463359:SFE-171; C020748:ferrous sulfate; C035885:ferric phosphate