A method for measuring cerebral glucose metabolism in vivo by 13C-NMR spectroscopy.
Current methods for estimating the rate of cerebral glucose utilization (CMR(glc)) typically measure metabolic activity for 40 min or longer subsequent to administration of [(13)C]glucose, 2-[(14)C]deoxyglucose, or 2-[(18)F]deoxyglucose. We report preliminary findings on estimating CMR(glc) for a period of 15 min by use of 2-[6-(13)C]deoxyglucose. After a 24-hr fast, rats were anesthetized, infused with [1-(13)C]glucose for 50 min, and injected with 2-[6-(13)C]deoxyglucose (500 mg/kg). During the subsequent 12.95 min the estimated value of CMR(glc) was 0.6 +/- 0.4 micromol/min/g (mean +/- SD, N = 7), in agreement with values reported for anesthetized rats studied with the 2-[(14)C]deoxyglucose method and other (13)C-NMR methods that measure CMR(glc). In rats injected with bicuculline methiodide (a known stimulant of CMR(glc)), CMR(glc) increased by more than 75% during 12.95 min following injection of bicuculline (Wilcoxon signed rank test, P = 0.042, N = 8).
Cohen
David M
DM
Children's Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030, USA. dcohen@bmc.tmc.edu
Wei
Jingna
J
O'Brian Smith
E
E
Gao
Xiaolian
X
Quast
Michael J
MJ
Sokoloff
Louis
L
D000818:Animals; D001692:Biological Transport; D001812:Blood-Brain Barrier; D001921:Brain / Q000378:metabolism*; D002560:Cerebrovascular Circulation; D003847:Deoxyglucose / Q000378:metabolism* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D023421:Models, Animal; D011336:Probability; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity
D003847:Deoxyglucose