title: Structure-based thermodynamic analysis of a coupled metal binding-protein folding reaction involving a zinc finger peptide.
abstract: The thermodynamics of metal binding by the prototypical Cys(2)His(2) zinc finger peptide CP-1 has been examined through the use of isothermal titration calorimetry. In cholamine buffer at pH 7.0, the binding of zinc(II) to CP-1 shows an enthalpy change of DeltaH degrees (obs) = -33.7 +/- 0.8 kcal/mol. Between one and two protons appear to be released accompanying the metal binding process. The heat of protonation of the cholamine buffer used is quite large (-11.5 kcal/mol), indicating that a portion of the observed metal binding enthalpy is due to buffer protonation. Structure-based thermodynamic analysis including the effect of water release from zinc(II) appears to account for the entropy associated with the coupled metal binding-protein folding process semiquantitatively. The strongest driving force for the reaction is the enthalpy associated with the four bonds from zinc(II) to cysteinate and histidine residues, compared with the bonds from zinc(II) to water. The binding of cobalt(II) to CP-1 is less enthalpically driven than the binding of zinc(II) by -7.6 kcal/mol. This value is approximately equal to, but slightly larger than, the expectation based on considerations of ligand field stabilization energy.
authors.affiliation: Department of Chemistry, Johns Hopkins University, Baltimore, Maryland 21218, USA.
authors.forename: Cheryl A
authors.initials: CA
authors.lastname: Blasie
authors.forename: Jeremy M
authors.initials: JM
authors.lastname: Berg
mesh_terms: D002151:Calorimetry; D003035:Cobalt / Q000737:chemistry*; D008956:Models, Chemical; D009842:Oligopeptides / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry*; D011485:Protein Binding; D017510:Protein Folding; D011522:Protons; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D015032:Zinc / Q000737:chemistry*; D016335:Zinc Fingers
chemical_list: C106243:CP-1 peptide; D009842:Oligopeptides; D011522:Protons; D003035:Cobalt; D015032:Zinc