title: Nonspecific, nested suppression PCR method for isolation of unknown flanking DNA ("cold-start method").
authors.affiliation: Department of Molecular Biosciences, Adelaide University, Australia.
authors.forename: Michael
authors.initials: M
authors.lastname: Lardelli
mesh_terms: D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers; D005784:Gene Amplification / Q000235:genetics; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D004247:DNA