title: Analysis of an anti-inflammatory steroidal drug, difluprednate, in aqueous humor by combination of semi-micro HPLC and column switching method.
abstract: A specific and sensitive method for the determination for difluprednate (DFBA) and its metabolite (deacetylated DFBA, DFB) in aqueous humor was developed. DFBA and DFB were initially absorbed on a Pinkerton-type column, then analyzed by high-performance liquid chromatography using a semi-micro column after column switching. Under the optimized conditions, calibration curves for DFBA and DFB showed good linearity over the range of 1.0-50 and 0.5-50 ng/ml, respectively. We applied the method to the analysis of DFBA and DFB in rabbit aqueous humor, and found that DFBA in rabbit aqueous humor 1 h after instillation of 0.002% DFBA ophthalmic emulsion was not detected, but DFB was present at the concentration of 4.3+/-3.1 ng/ml.
authors.affiliation: Senju Pharmaceutical Co., Ltd. 2-5-8, Hiranomachi, Chuo-Ku, Osaka 541-0046, Japan.
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Yasueda
authors.forename: Masako
authors.initials: M
authors.lastname: Kimura
authors.forename: Akira
authors.initials: A
authors.lastname: Ohtori
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Kakehi
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis* / Q000378:metabolism; D001082:Aqueous Humor / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D005477:Fluprednisolone / Q000031:analogs & derivatives* / Q000032:analysis* / Q000378:metabolism; D011817:Rabbits; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000379:methods*
chemical_list: D000893:Anti-Inflammatory Agents; D005477:Fluprednisolone; C015808:difluprednate