title: Serum cytokine concentration after liver lobe harvesting for transplantation.
abstract: OBJECTIVE
  To investigate cytokine concentration after liver lobe harvesting for transplantation in order to prove that it is a relatively safe surgical procedure with limited tissue injury.
  
  
  METHODS
  Study was performed in 17 healthy liver donors and 6 patients with benign liver tumors. Serum levels of IL-6, IL-1ra and sTNFRI were measured before surgery and on the days 1, 3 and 7 thereafter, by immunoassay (ELISA).
  
  
  RESULTS
  There was a significant increase in serum concentrations of IL-6 on day 1-7 (from 2.4 pg/ml to 315.1 +/- 526, 47.3 +/- 48 and 15.3 +/- 15 pg/ml; p = 0.0002, p = 0.0006, p = 0.003), for IL-1ra on day 1-3 (from 472.5 +/- 436 pg/ml to 2072.6 +/- 3511 and 715.5 +/- 268 pg/ml; p = 0.001, p = 0.004), and for sTNFRI on day 1-3 (from 1075.7 +/- 338.0 pg/ml to 1601.4 +/- 317.0 and 1528.9 +/- 402.0 pg/ml; p = 0.0006, p = 0.003), following liver harvesting. No significant difference was observed between pre and postoperative IL-6 serum concentration, whereas IL-1ra and sTNFRI were elevated only on day 3 after liver resection (p = 0.02, p = 0.04). A significantly higher level of sTNFRI was seen in patients after liver resection, as compared to liver donors on day 1 (p = 0.01), 3 (p = 0.03) and 7 (p = 0.0006) after surgery, whereas on day 3 (p = 0.03) and 7 (p = 0.01) when IL-1ra was measured.
  
  
  CONCLUSIONS
  The short period of elevated concentrations of IL6, sTNFRI and IL1ra after harvesting of lobes for transplantation and a relatively low serum level of these cytokines indicate that this type of procedure does not evoke any major postoperative SIRS-type reaction as seen not infrequently after resection of liver for primary or secondary liver pathology.
authors.affiliation: Dept. of Surgical Research & Transplantology, Medical Research Center, Polish Academy of Science, Poland. slotwinski@cmdik.pan.pl
authors.forename: R
authors.initials: R
authors.lastname: Słotwiński
authors.forename: W L
authors.initials: WL
authors.lastname: Olszewski
authors.forename: R
authors.initials: R
authors.lastname: Paluszkiewicz
authors.forename: K
authors.initials: K
authors.lastname: Zieniewicz
authors.forename: P
authors.initials: P
authors.lastname: Hevelke
authors.forename: M
authors.initials: M
authors.lastname: Zaleska
authors.forename: M
authors.initials: M
authors.lastname: Krawczyk
authors.forename: I W
authors.initials: IW
authors.lastname: Krasnodebski
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000097:blood; D016207:Cytokines / Q000097:blood*; D005260:Female; D006498:Hepatectomy; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D015850:Interleukin-6 / Q000097:blood; D016031:Liver Transplantation; D008297:Male; D011184:Postoperative Period; D018124:Receptors, Tumor Necrosis Factor / Q000097:blood; D047888:Receptors, Tumor Necrosis Factor, Type I; D012795:Sialoglycoproteins / Q000097:blood; D013997:Time Factors; D014019:Tissue Donors; D020858:Tissue and Organ Harvesting / Q000379:methods*
chemical_list: D015703:Antigens, CD; D016207:Cytokines; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D015850:Interleukin-6; D018124:Receptors, Tumor Necrosis Factor; D047888:Receptors, Tumor Necrosis Factor, Type I; D012795:Sialoglycoproteins