A DNA probe specific for Aeromonas colonies.
Members of the genus Aeromonas are important enteropathogens. Commercial identification systems are often unable to correctly identify Aeromonas strains and misidentification as Vibrio spp. is common. A digoxigenin-DNA probe based on a 237 bp of the glycerophospholipid-cholesterol acyltransferase gene has been tested in a colony hybridization assay. The probe hybridized with all Aeromonas species tested (n = 16) but not with strains of other enteropathogenic bacteria (n = 20). The probe allowed the unequivocal identification of Aeromonas in primary isolation media within 36 h.
Chacón
M R
MR
Departamento de Ciencias Médicas Básicas, Facultad de Medicina y Ciencias de la Salud, Universidad Rovira y Virgili, San Lorenzo 21, 43201, Reus, Spain.
Castro-Escarpulli
G
G
Soler
L
L
Guarro
J
J
Figueras
M J
MJ
D000217:Acyltransferases / Q000235:genetics*; D000333:Aeromonas / Q000145:classification* / Q000235:genetics / Q000254:growth & development*; D000818:Animals; D003470:Culture Media; D015342:DNA Probes; D004076:Digoxigenin; D006168:Guinea Pigs; D006801:Humans; D008049:Lipase / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction; D013045:Species Specificity
D003470:Culture Media; D015342:DNA Probes; D000217:Acyltransferases; C085461:Aeromonas hydrophilia lipase-acyltransferase; D008049:Lipase; D004076:Digoxigenin