Comparative study on the application of capillary liquid chromatography and capillary electrochromatography for investigation of enantiomeric purity of the contraceptive drug levonorgestrel.
In the last few years, it has been shown that capillary electrochromatography (CEC) is a promising technique for enantioseparations. However, to date almost no studies are published on a critical comparison of CEC and its pressure-driven counterpart, capillary liquid chromatography (CLC) for real samples. In this study, the goal was to compare CLC and CEC for the determination of the enantiomeric purity of the contraceptive drug levonorgestrel and its pharmaceutical formulation. The study prevailed that not all potential advantages of CEC over CLC can easily be transformed in a real gain of detection limit of the enantiomeric impurity. However, certain advantages of CEC over CLC have been unambiguously shown.
Chankvetadze
Bezhan
B
Institute of Pharmaceutical and Medicinal Chemistry, University of Münster, Hittorfstrasse 58-62, D-48149 Münster, Germany. chankve@uni-muenster.de
Kartozia
Irma
I
Yamamoto
Chiyo
C
Okamoto
Yoshio
Y
Blaschke
Gottfried
G
D002853:Chromatography, Liquid / Q000379:methods; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003280:Contraceptives, Oral, Synthetic / Q000032:analysis* / Q000737:chemistry; D004340:Drug Contamination; D016912:Levonorgestrel / Q000032:analysis* / Q000737:chemistry; D013237:Stereoisomerism
D003280:Contraceptives, Oral, Synthetic; D016912:Levonorgestrel