Serotonin regulates hippocampal glucocorticoid receptor expression via a 5-HT7 receptor.
Glucocorticoid receptor expression in primary hippocampal cell cultures was significantly increased with either 10 mM 8-bromo cAMP, 50 nM 5-carboxamidotryptamine (5-CT), a potent 5-HT7 receptor agonist, or 100 nM 5-HT. The effect of 5-HT or 5-CT was blocked with methiothepin or by a protein kinase A inhibitor, but not pindolol. These results suggest that the effects of 5-HT on hippocampal GR expression is mediated by a 5-HT7 receptor.
Laplante
Patricia
P
Developmental Neuroendocrinology Laboratory, Douglas Hospital Research Center, Departments of Psychiatry and Neurology & Neurosurgery, McGill University, 6875 Boul. LaSalle, Quebec, Montréal, Canada H4H 1R3.
Diorio
Josie
J
Meaney
Michael J
MJ
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005246:Feedback / Q000502:physiology; D005333:Fetus; D006185:Habituation, Psychophysiologic / Q000502:physiology; D006234:Handling, Psychological; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats; D020318:Rats, Long-Evans; D011965:Receptors, Glucocorticoid / Q000187:drug effects / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000187:drug effects / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism* / Q000494:pharmacology; D012702:Serotonin Antagonists / Q000494:pharmacology; D017366:Serotonin Receptor Agonists / Q000494:pharmacology; D013312:Stress, Physiological / Q000378:metabolism* / Q000503:physiopathology
D004791:Enzyme Inhibitors; D011965:Receptors, Glucocorticoid; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D017366:Serotonin Receptor Agonists; C104425:serotonin 7 receptor; D015124:8-Bromo Cyclic Adenosine Monophosphate; D012701:Serotonin; D003346:Corticotropin-Releasing Hormone; D000242:Cyclic AMP