The roles of p53, DNA repair, and oxidative stress in ultraviolet C induction of proliferating cell nuclear antigen expression.
The expression of proliferating cell nuclear antigen (PCNA) promoter was moderately induced in UV-irradiated, quiescent human and rodent cells. The induction was independent of tumor suppressor gene p53, because the PCNA expression was UV-inducible in the subclones of human fibroblasts in which the activity of p53 was abrogated by human papilloma virus E6. Furthermore, the induction did not depend on DNA repair, since PCNA was UV inducible in UVL-10 and xrs-6 cells, in which nucleotide excision repair and double-stranded repair, respectively, are largely compromised. However, the induction was inhibited by antioxidant N-acetylcysteine. The role of oxidative stress observed here is consistent with the previous finding that the proximal AP-1 site is critical to the UV inducibility of PCNA promoter.
Chang
Yu-Ching
YC
Department of Life Science, National Tsing Hua University, Hsin-Chu 30043, Taiwan. ycliu@life.nthu.edu.tw
Chang
Hsueh-Wei
HW
Liao
Chu-Bin
CB
Liu
Yin-Chang
YC
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D017931:DNA Primers; D004260:DNA Repair / Q000187:drug effects*; D005347:Fibroblasts / Q000502:physiology* / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000528:radiation effects*; D016158:Genes, p53 / Q000528:radiation effects; D006801:Humans; D007231:Infant, Newborn; D007700:Kinetics; D008297:Male; D018384:Oxidative Stress; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics* / Q000528:radiation effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin; D014466:Ultraviolet Rays
D017931:DNA Primers; D018809:Proliferating Cell Nuclear Antigen