title: Identification of Ruscus steroidal saponins by HPLC-MS analysis.
abstract: A novel HPLC-UV method has been developed for the fingerprint analysis of the steroidal saponins in the rhizomes of three Ruscus species (Ruscus aculeatus, Ruscus hypoglossum and Ruscus colchicus). Saponins were identified by HPLC-ESI-MS. During the study a new major saponin was detected in the rhizomes of R. hypoglossum and R. colchicus. The structure of the new compound was defined as 1-O-[alpha-L-rhamnopyranosyl-(1-->2)-6-O-acetyl-beta-D-galactopyranosyl]-1beta,3beta,22xi,26-tetrahydroxy-furost-5(6)-en-26-O-beta-D-glucopyranoside (8) by spectral analysis.
authors.affiliation: Indena SpA, Research and Development Laboratories, Via Don Minzoni 6, 20090 Settala (MI), Italy.
authors.forename: E
authors.initials: E
authors.lastname: de Combarieu
authors.forename: M
authors.initials: M
authors.lastname: Falzoni
authors.forename: N
authors.initials: N
authors.lastname: Fuzzati
authors.forename: F
authors.initials: F
authors.lastname: Gattesco
authors.forename: A
authors.initials: A
authors.lastname: Giori
authors.forename: M
authors.initials: M
authors.lastname: Lovati
authors.forename: R
authors.initials: R
authors.lastname: Pace
mesh_terms: D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D031443:Ruscus / Q000737:chemistry* / Q000145:classification; D012503:Saponins / Q000032:analysis* / Q000737:chemistry*
chemical_list: C473408:1-O-(rhamnopyranosyl-(1-2)-6-O-acetyl-galactopyranosyl)-1,3,22,26-tetrahydroxy-furost-5(6)-en-26-O-glucopyranoside; D012503:Saponins