Effects of selenium and iodine on the expression of c-fos and c-jun mRNA and their proteins in cultured rat hippocampus neurons.
OBJECTIVE
To study the effect of selenium (Se) and iodine (I) and the compound of both on the proto-oncogenes c-fos and c-jun mRNA and their protein expression in the cultured rat hippocampus neurons.


METHODS
Using the technique of serum free hippocampus neuron culture, different doses of Se and I and Se + I compound were added into the medium. The expression of the mRNA of c-fos, c-jun in hippocampus neurons cultured for 1, 3, 5, 7 and 10 d were studied using both in situ hybridization and SABC immunohistochemical technique.


RESULTS
Both Se and I could enhance the expression of c-fos, c-jun mRNA and their proteins, especially the combination of I and Se able to give a remarkable effect on c-jun mRNA expression.


CONCLUSIONS
Se and I may effect the expression of both c-fos and c-jun mRNA, especially the c-jun mRNA and its protein of hippocampus neurons, and thus may effect the differentiation and development of neurons.
Tian
Dongping
D
Department of Pathology, Shantou University Medical College, Shantou 515031, China (Email: minsu@ncrc.stu.edu.cn)
Su
Min
M
Wu
Xianying
X
Li
Qiaoshan
Q
Zheng
Ruiming
R
Li
Guangyuan
G
Song
Tianbao
T
Xu
Xiaohu
X
D000818:Animals; D004268:DNA-Binding Proteins / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D007455:Iodine; D009474:Neurons / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012643:Selenium
D004268:DNA-Binding Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D007455:Iodine; D012643:Selenium