In-frame cloning of synthetic genes using PCR inserts.
Pierce
James C
JC
University of the Sciences in Philadelphia, Philadelphia, PA, USA.
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers / Q000138:chemical synthesis / Q000737:chemistry; D015195:Drug Design; D005813:Genes, Synthetic / Q000235:genetics*; D007202:Indicators and Reagents; D016254:Mutagenesis, Insertional / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011506:Proteins / Q000235:genetics; D015183:Restriction Mapping
D017931:DNA Primers; D007202:Indicators and Reagents; D011506:Proteins