title: Expression of receptor activator of NF-kappa B ligand and osteoprotegerin in culture of human periodontal ligament cells.
abstract: The receptor activator of NF-kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG), are the important proteins implicated in osteoclastogenesis. In this study, we investigated the expressions of RANKL and OPG in cultured human periodontal ligament (PDL) cells and their roles in osteoclastogenesis. Northern blotting revealed that the OPG mRNA was down-regulated remarkably by application of 10-8 m one-alpha, 25-dihydroxyvitamin D3[1,25-(OH)2D3] and 10-7 m dexamethasone (Dex). In contrast, RANKL mRNA was up-regulated by the same treatment. Western blotting demonstrated decrease of OPG by the application of 1,25-(OH)2D3 and Dex. Tartrate-resistant acid phosphatase-positive multinuclear cells were markedly induced when the PDL cells were cocultured with mouse bone marrow cells in the presence of an anti-OPG antibody together with 1,25-(OH)2D3 and Dex. These results indicate that PDL cells synthesize both RANKL and OPG and that inactivation of OPG may play a key role in the differentiation of osteoclasts.
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Hokkaido University, Sapporo, Hokkaido, Japan. hasegawa@den.hokudai.ac.jp
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: Takashi
authors.initials: T
authors.lastname: Kikuiri
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Yawaka
authors.forename: Sadaaki
authors.initials: S
authors.lastname: Takeyama
authors.forename: Akira
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Oguchi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Shirakawa
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D015152:Blotting, Northern; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002117:Calcitriol / Q000494:pharmacology; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D016207:Cytokines / Q000032:analysis / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D015536:Down-Regulation; D005786:Gene Expression Regulation; D005938:Glucocorticoids / Q000494:pharmacology; D006023:Glycoproteins / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000235:genetics; D051379:Mice; D016328:NF-kappa B / Q000032:analysis* / Q000235:genetics; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism; D053244:Osteoprotegerin; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D053245:RANK Ligand; D012333:RNA, Messenger / Q000235:genetics; D053246:Receptor Activator of Nuclear Factor-kappa B; D018160:Receptors, Cytoplasmic and Nuclear / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000235:genetics; D018124:Receptors, Tumor Necrosis Factor / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013223:Statistics as Topic; D000071681:Tartrate-Resistant Acid Phosphatase; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000235:genetics; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; D016207:Cytokines; D005938:Glucocorticoids; D006023:Glycoproteins; D007527:Isoenzymes; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; D053246:Receptor Activator of Nuclear Factor-kappa B; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; C505686:TNFRSF11A protein, human; C506030:TNFRSF11B protein, human; C505688:TNFSF11 protein, human; C505687:Tnfrsf11a protein, mouse; C506031:Tnfrsf11b protein, mouse; C505689:Tnfsf11 protein, mouse; D014409:Tumor Necrosis Factor-alpha; D003907:Dexamethasone; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D002117:Calcitriol