title: Two efficient polymeric chemical platforms for oligonucleotide microarray preparation.
abstract: In this report we describe two robust procedures for oligonucleotide microarray preparation based on polymeric coatings. The proposed chemical approaches include: 1) a glass functionalisation step with appropriate silanes (gamma-aminopropyltriethoxysilane-APTES or 3-glycidoxypropyltrimethoxysilane-GOPS), 2) a coating step using polymers (poly-L-Lysine or poly(acrylic acid-co-acrylamide) copolymer) covalently bound to the modified glass and 3) a surface activation step to allow for the attachment of amino-modified oligonucleotides. Results obtained using these chemistries in oligo microarray preparation show: 1) an overall high loading capacity and availability to hybridisation against targets, 2) a good uniformity, 3) resistance to consecutive probing/ stripping cycles, 4) stability to thermal cycles, 5) effectiveness in hybridisation-mediated mutation detection procedures and 6) the possibility to perform enzymatic reactions, such as ligation.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi di Milano, LITA, Via Fratelli Cervi 93, 20090 Segrate Mi, Italy.
authors.forename: Clarissa
authors.initials: C
authors.lastname: Consolandi
authors.forename: Bianca
authors.initials: B
authors.lastname: Castiglioni
authors.forename: Roberta
authors.initials: R
authors.lastname: Bordoni
authors.forename: Elena
authors.initials: E
authors.lastname: Busti
authors.forename: Cristina
authors.initials: C
authors.lastname: Battaglia
authors.forename: Luigi Rossi
authors.initials: LR
authors.lastname: Bernardi
authors.forename: Gianluca
authors.initials: G
authors.lastname: De Bellis
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004252:DNA Mutational Analysis / Q000379:methods; D019398:Genes, BRCA1; D005898:Glass / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D009154:Mutation / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes / Q000737:chemistry* / Q000235:genetics; D011108:Polymers / Q000737:chemistry*; D011437:Propylamines; D012821:Silanes / Q000737:chemistry*
chemical_list: D000178:Acrylamides; D015345:Oligonucleotide Probes; D011108:Polymers; D011437:Propylamines; D012821:Silanes; C431184:poly(acrylamide-co-acrylic acid); C477625:amino-propyl-triethoxysilane