Different role of saturated and unsaturated fatty acids in beta-cell apoptosis.
It is believed that free fatty acids contribute to the pathogenesis of type 2 diabetes in humans. We have recently shown that lipoapoptosis of human beta-cells is specifically induced by saturated fatty acids while unsaturated had no effect. In the present study we tested the effect of co-incubation of different saturated and unsaturated free fatty acids on lipoapoptosis in beta-cells. RIN1046-38 cells and isolated human beta-cells were incubated with combinations of saturated fatty acids (palmitate, stearate) and mono- or polyunsaturated fatty acids (palmitoleate, oleate, and linoleate). Cells were incubated for 24-72 h with 1mM fatty acids. All unsaturated fatty acids tested completely prevented palmitate- or stearate-induced apoptosis of rat and human beta-cells as assessed by flow cytometric cell cycle analysis and TUNEL assay. This might suggest that apoptosis in vivo is predominantly determined by the content of unsaturated fatty acids in a mixed fatty acid pool.
Eitel
Katrin
K
Internal Medicine IV, University of Tübingen, Otfried-Müller-Str. 10, D-72076 Tübingen, Germany.
Staiger
Harald
H
Brendel
Mathias D
MD
Brandhorst
Daniel
D
Bretzel
Reinhard G
RG
Häring
Hans-Ulrich
HU
Kellerer
Monika
M
D017209:Apoptosis; D002478:Cells, Cultured; D005227:Fatty Acids / Q000037:antagonists & inhibitors / Q000494:pharmacology* / Q000502:physiology; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology* / Q000502:physiology; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects; D008041:Linoleic Acids / Q000494:pharmacology; D009829:Oleic Acids / Q000494:pharmacology; D010169:Palmitic Acids / Q000494:pharmacology; D013229:Stearic Acids / Q000037:antagonists & inhibitors; D014407:Tumor Cells, Cultured
D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D009829:Oleic Acids; D010169:Palmitic Acids; D013229:Stearic Acids; C008757:palmitoleic acid; C031183:stearic acid