Nicotinamide "protects" resting lymphocytes exposed to hydrogen peroxide from necrosis but not from apoptosis.
The aim of this work was to investigate the relationship between mechanisms of DNA repair and apoptosis induced by oxidative stress (H2O2) in human lymphocytes. Using the comet assay, fluorescent microscopy, and DNA electrophoresis, we studied the DNA damage induced by hydrogen peroxide (H2O2) treatment, the time and the amount of repair of strand breaks, the type of cell death, and the influence of inhibitors of repair (nicotinamide). When lymphocytes were treated with H2O2, we observed an increased in necrosis compared to apoptosis. However, when nicotinamide (which inhibits DNA repair) was added, the mode of death reversed to increased apoptosis. These results indicate that nicotinamide "protects" resting lymphocytes exposed to H2O2 from necrosis but not from apoptosis.
Tronov
V A
VA
Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.
Konstantinov
E M
EM
Petrakou
E
E
Tsilimigaki
S
S
Piperakis
S M
SM
D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D004587:Electrophoresis, Agar Gel; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects* / Q000473:pathology; D009336:Necrosis; D009536:Niacinamide / Q000494:pharmacology*
D009536:Niacinamide; D006861:Hydrogen Peroxide