Elucidation of structure-function relationships in the protein subunit of bacterial RNase P using a genetic complementation approach.
RNase P is a ribonucleoprotein involved in tRNA biosynthesis in all living organisms. Bacterial RNase P is comprised of a catalytic RNA subunit and a lone protein cofactor which plays a supporting, albeit essential, role in the tRNA processing reaction in vivo. In this study, we have searched various databases to identify homologs of the protein subunit of RNase P from diverse bacteria and used an alignment of their primary sequences to determine the most highly conserved residues, and thereby extend earlier predictions of which residues might play an important role in RNA recognition. By employing a genetic complementation assay, we have also gained insights into structure- function relationships in the protein subunit of bacterial RNase P.
Jovanovic
Milan
M
Department of Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
Sanchez
Ruth
R
Altman
Sidney
S
Gopalan
Venkat
V
D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D015153:Blotting, Western; D017124:Conserved Sequence; D030562:Databases, Protein; D004722:Endoribonucleases / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D029968:Escherichia coli Proteins; D005816:Genetic Complementation Test; D008954:Models, Biological; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D017433:Protein Structure, Secondary; D021122:Protein Subunits; D016337:RNA, Catalytic / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D043262:Ribonuclease P; D016415:Sequence Alignment; D013329:Structure-Activity Relationship
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D021122:Protein Subunits; D016337:RNA, Catalytic; D016601:RNA-Binding Proteins; D004722:Endoribonucleases; D043262:Ribonuclease P; C470865:ribonuclease P, E coli