[Morphological and immunohistochemical studies in testes of normal and cryptorchid boars].
Scrotal (n = 66), inguinal (n = 7) and abdominal (n = 36) testes from pigs differing in age (from 1 day to 4 years) were collected after slaughtering or by castration, fixed in 4% formalin and examined by light microscopy. The expression of vimentin, desmin, alpha-actin and PCNA was detected by immunohistochemical technique. Histologically no differences between scrotal and cryptorchid testes are obvious during the prepubertal period, until the age of 10-12 weeks, but with the onset of puberty degenerative alterations (Sertoli-cell-only morphology, giant cells) in dystopic testes occur. Additionally the differentiation of the interstitial cells is disturbed in abdominal testes resulting in a deviating expression pattern of alpha-actin. The Sertoli cells show different distribution patterns (basal, perinuclear, apical) of vimentin depending on the position of the testis and the age of the animal.
Loose
Helge
H
Ambulatorische und Geburtshilfliche Tierklinik, Universität Leipzig.
Aupperle
Heike
H
Schnurrbusch
Ute
U
Schoon
Heinz-Adolf
HA
D000199:Actins / Q000378:metabolism; D000367:Age Factors; D000818:Animals; D003456:Cryptorchidism / Q000473:pathology / Q000662:veterinary*; D003893:Desmin / Q000378:metabolism; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000473:pathology*; D013737:Testis / Q000033:anatomy & histology / Q000737:chemistry / Q000473:pathology*; D014746:Vimentin / Q000378:metabolism
D000199:Actins; D003893:Desmin; D018809:Proliferating Cell Nuclear Antigen; D014746:Vimentin