Studies on Bacillus stearothermophilus. Part II. Transformation of progesterone.
Bacillus stearothermophilus, a thermophilic bacterium isolated from Kuwaiti desert, when incubated with exogenous progesterone for 10 days at 65 degrees C produced two new dihydroxy isomers of progesterone, and two known compounds, 5 alpha-pregnane-3,6,20-trione and 6-dehydroprogesterone, along with the earlier reported monohydroxylated metabolites and a B-Seco compound. The two new dihydroxy compounds were identified as 6 alpha,20 alpha-dihydroxyprogesterone and 6 beta,20 alpha-dihydroxyprogesterone. These metabolites were purified by TLC and HPLC followed by their identification through 1H, 13C NMR and other spectroscopic data.
Al-Awadi
Sameera
S
Biochemistry Program, Department of Biological Sciences, Faculty of Science, Kuwait University, Safat, Kuwait. sameera@kuc01.kuniv.edu.kw
Afzal
M
M
Oommen
S
S
D001711:Biotransformation; D002845:Chromatography; D001411:Geobacillus stearothermophilus / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011374:Progesterone / Q000737:chemistry / Q000378:metabolism*
D011374:Progesterone