title: Study of isolation of fluoroquinolone-resistant Ureaplasma urealyticum and identification of mutant sites.
abstract: OBJECTIVE
  To study the resistance mechanism of clinical isolates of Ureaplasma urealyticum resistant to fluoroquinolones.
  
  
  METHODS
  Thirteen isolates of Ureaplasma urealyticum resistant to six fluoroquinolones were selected out of 184 clinical isolates and their QRDRs (quinolone resistance-determining region) gyrA, gyrB, parC and parE were amplified by PCR. Sequencing results were compared to those susceptible reference strains and a comparison of deduced amino acid sequences were performed.
  
  
  RESULTS
  Sequence comparison revealed a C to A change at 87nt of gyrA QRDR leading to the substitution of Asp95 with glutamic acid and a C to T change at 50nt of parC QRDR leading to the substitution of Ser80 with leucine.
  
  
  CONCLUSIONS
  These results suggest that a C to A change at 87nt of gyrA QRDR and a C to T change at 50nt of parC QRDR are associated with fluoroquinolone resistance of Ureaplasma urealyticum.
authors.affiliation: Department of Microbiology, Basic Medical College, Nanhua University, Hengyang 421001, Hunan Province, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Zhang
authors.forename: Yimou
authors.initials: Y
authors.lastname: Wu
authors.forename: Weiguo
authors.initials: W
authors.lastname: Yin
authors.forename: Minjun
authors.initials: M
authors.lastname: Yu
mesh_terms: D019943:Amino Acid Substitution; D000890:Anti-Infective Agents / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D024841:Fluoroquinolones; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D016990:Ureaplasma urealyticum / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase