title: The anti-proliferative gene TIS21 is involved in osteoclast differentiation.
abstract: The remodeling process of bone is accompanied by complex changes in the expression levels of various genes. Several approaches have been employed to detect differentially-expressed genes in regard to osteoclast differentiation. In order to identify the genes that are involved in osteoclast differentiation, we used a cDNAarray-nylon membrane. Among 1,200 genes that showed a measurable signal, 19 genes were chosen for further study. Eleven genes were up-regulated; eight genes were downregulated. TIS21 was one of the up-regulated genes which were highly expressed in mature osteoclasts. To verify the cDNA microarray results, we carried out RT-PCR and real-time RT-PCR for the TIS21 gene. The TIS21 mRNA level was higher in differentiated-osteoclasts when compared to undifferentiated bone-marrow macrophages. Furthermore, the treatment with 1 mM of a TIS21 antisense oligonucleotide reduced the formation of osteoclasts from the bone-marrow-precursor cells by approximately 30%. These results provide evidence for the potential role of TIS21 in the differentiation of osteoclasts
authors.affiliation: National Research Laboratory for Bone Metabolism, Research Center for Proteineous Materials, Chosun University, Gwangju.
authors.forename: Soo Woong
authors.initials: SW
authors.lastname: Lee
authors.forename: Han Bok
authors.initials: HB
authors.lastname: Kwak
authors.forename: Hong Chan
authors.initials: HC
authors.lastname: Lee
authors.forename: Seung Ku
authors.initials: SK
authors.lastname: Lee
authors.forename: Hong-Hee
authors.initials: HH
authors.lastname: Kim
authors.forename: Zang Hee
authors.initials: ZH
authors.lastname: Lee
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation; D016147:Genes, Tumor Suppressor; D017874:Immediate-Early Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010010:Osteoclasts / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins; D015854:Up-Regulation
chemical_list: C489435:Btg2 protein, mouse; D018076:DNA, Complementary; D017874:Immediate-Early Proteins; D016376:Oligonucleotides, Antisense; D025521:Tumor Suppressor Proteins