Oxygenation of hypoxic region in solid tumor by administration of human serum albumin incorporating synthetic hemes.
Recombinant human serum albumin incorporating synthetic heme (rHSA-FeP) was tested for its ability to increase O(2) tension in the hypoxia of the solid tumor rat model. By the direct administration of the rHSA-FeP solution (10 mL/kg) via the aorta descendens to the ascites hepatoma LY80 tumor on the right femur, the O(2) tension of the hypoxic region immediately increased to 3.45 +/- 1.43 Torr, which corresponds to a 2.4-fold increase compared to that of the baseline value. These high O(2) levels continued for 300 s after the infusion. The rHSA-FeP solution can be utilized not only as a red blood cell substitute but also as an O(2)-carrying medicine for the effective reoxygenation of the hypoxia solid tumor.
Kobayashi
Koichi
K
Department of General Thoracic Surgery, School of Medicine, Keio University, Tokyo 160-8582, Japan.
Komatsu
Teruyuki
T
Iwamaru
Arifumi
A
Matsukawa
Yasuko
Y
Horinouchi
Hirohisa
H
Watanabe
Masazumi
M
Tsuchida
Eishun
E
D000818:Animals; D006418:Heme / Q000737:chemistry*; D000860:Hypoxia / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D008297:Male; D010100:Oxygen / Q000008:administration & dosage*; D051381:Rats; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000737:chemistry; D012709:Serum Albumin / Q000008:administration & dosage* / Q000737:chemistry; D014407:Tumor Cells, Cultured
D011994:Recombinant Proteins; D012709:Serum Albumin; D006418:Heme; D010100:Oxygen