Inducible nitric oxide synthase knockout mouse and low-density lipoprotein oxidation.
We have presented experimental procedures that examine macrophage-mediated LDL oxidation using Ham's F-10 medium. By comparing iNOS-/- and iNOS+/+ macrophages, an antioxidant effect for NO and a prooxidant effect for IFN-gamma were demonstrated. The methods outlined here should allow for the investigation on the mechanism of in vitro LDL oxidation and how the macrophage-mediated LDL oxidation process is affected by various factors, one of which was the effect of iNOS induction by IFN-gamma.
Niu
Xi-Lin
XL
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27705, USA.
Chen
Yi
Y
Shoyama
Yutaka
Y
Ishiwata
Kazuo
K
Obama
Ruriko
R
Nakazawa
Hiroe
H
D000818:Animals; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000502:physiology*; D052247:Nitric Oxide Synthase Type II; D010084:Oxidation-Reduction
D008077:Lipoproteins, LDL; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse