title: Lymphomas of the bone: a pathological and clinical study of 54 cases.
abstract: We examined 28 cases of primary bone lymphomas (PBL; stage IE) and 26 cases of systemic lymphomas involving the bone (SBL; stage IIE to IV). Two histologic types were prevalent: Diffuse large B-cell lymphomas (DLBCL; 26 PBL and 21 SBL) and CD30+ anaplastic large cell lymphomas (ALCL; 1 PBL and 4 SBL). A mature B phenotype (CD45+, CD20+, CD79a+, CDw75+/-, CD10-/+) was established in the DLBCL group. Bcl-2 immunoreactivity was demonstrated in 13/37 cases (35%), and bcl-6 immunostaining was observed in 22/32 cases (69%). ALCL showed null/T phenotype (CD3-/+; CD43+/-; CD30+), with ALK-1 expression in 3/3 cases. With use of a FR3A primer, a monoclonal pattern was demonstrated by PCR analysis in 22/41 lymphomas (54%). Bcl-2 translocation was identified in 2/41 cases (5%). This study details the clinical and pathological characteristics of bone lymphomas. Our immunohistochemical and molecular data suggest that most of them are "de novo" DLBCL and support their follicle center origin.
authors.affiliation: Departement of Medicine, Surgery and Dental Sciences, AO S Paolo (HSP), Milan, Italy.
authors.forename: Umberto
authors.initials: U
authors.lastname: Gianelli
authors.forename: Carlo
authors.initials: C
authors.lastname: Patriarca
authors.forename: Alessia
authors.initials: A
authors.lastname: Moro
authors.forename: Maurilio
authors.initials: M
authors.lastname: Ponzoni
authors.forename: Roberto
authors.initials: R
authors.lastname: Giardini
authors.forename: Maura
authors.initials: M
authors.lastname: Massimino
authors.forename: Rosa Maria
authors.initials: RM
authors.lastname: Alfano
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Armiraglio
authors.forename: Paolo
authors.initials: P
authors.lastname: Nuciforo
authors.forename: Silvano
authors.initials: S
authors.lastname: Bosari
authors.forename: Guido
authors.initials: G
authors.lastname: Coggi
authors.forename: Antonina
authors.initials: A
authors.lastname: Parafioriti
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D001859:Bone Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D002648:Child; D002999:Clone Cells; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D015321:Gene Rearrangement; D019254:Genes, bcl-2 / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D016403:Lymphoma, Large B-Cell, Diffuse / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D017728:Lymphoma, Large-Cell, Anaplastic / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D016016:Proportional Hazards Models
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm