Characterization of AnRP-mediated negative regulation of the xylanase gene, cgxA, from Chaetomium gracile in Aspergillus nidulans.
OBJECTIVE
In vivo regulatory features of AnRP, a repressor for the cgxA gene, were characterized.


RESULTS
Titration of AnRP by introducing multiple copies of its specific binding sequence 'TTGACAAAT' into an Aspergillus nidulans strain containing the Chaetomium gracile cgxA gene enhanced the cgxA gene expression. AnRP functions independently of and cooperatively with CreA to modulate the cgxA gene expression.


CONCLUSIONS
AnRP is a CreA-independent negative regulatory factor controlling the cgxA expression.


CONCLUSIONS
Xylanases of filamentous fungi have received increased interest because of their potential biotechnological applications. Elucidation of the factors involved in the regulation of the xylanolytic genes in fungi will help to increase levels of xylanase production.
Rao
U
U
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
Kato
M
M
Kobayashi
T
T
Tsukagoshi
N
N
D000681:Amylases / Q000096:biosynthesis; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000145:classification; D002597:Chaetomium / Q000201:enzymology*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004794:Enzyme Repression; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D043325:Xylan Endo-1,3-beta-Xylosidase; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000096:biosynthesis / Q000235:genetics*
D002241:Carbohydrates; D004271:DNA, Fungal; D012097:Repressor Proteins; D014990:Xylans; D000681:Amylases; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; D005947:Glucose