Production and characterization of tannase from Bacillus cereus KBR9.
A tannase-producing soil bacteria has been isolated and identified as Bacillus cereus. It can degrade tannic acid and produce maximum tannase (0.22 U/ml) at stationary phases of growth (24 h). Maximum growth and enzyme production occurred with initial medium pH of 4.5-5.0. Partial purified tannase showed optimum activity at pH 4.5 and 40 degrees C. It remains stable up to 30 degrees C and pH 4.5 to 5.0. The enzyme is salt tolerant, stable up to 2 m of NaCl and retains 82% original activity in 3 m.
Mondal
Keshab C.
KC
Microbiology Laboratory, Department of Botany and Forestry, Vidyasagar University, Midnapore 721 102, West Bengal, India.
Banerjee
Debdulal
D
Banerjee
Rintu
R
Pati
Bikas R.
BR