title: Serum levels of IL-1 beta, TNF-alpha, IL-8, and acute phase proteins in seronegative spondyloarthropathies.
abstract: OBJECTIVE
  Some immunological abnormalities have been described in seronegative spondyloarthropathies (SpA). The aim of this study is to determine the serum levels of IL-1beta, TNF-alpha and IL-8, which are proinflammatory cytokines in active and inactive patients with SpA, to compare the results with those of controls and to investigate a relationship with clinical activity and acute phase proteins.
  
  
  METHODS
  Forty-two patients (34 males and eight females) and 22 healthy controls (17 M and 5 F) were included in the study. All patients fulfilled Amor criteria for the classification of SpA. Among patients 23 had active and 19 had inactive disease. IL-1beta, TNF-alpha and IL-8 were determined by enzyme-linked immunosorbent assay ( ELISA), acute phase proteins were measured by nephelometric assay.
  
  
  RESULTS
  There was no statistically significant difference between mean IL-1beta levels of patient groups and controls. Serum mean TNF-a levels in active and inactive patients were significantly increased as compared to that in the controls (P < 0.05, P < 0.05, respectively). Serum mean IL-8 levels in active patients was significantly increased as compared to that in the controls and in inactive patients (P < 0.01, P < 0.01, respectively). High serum IL-8 levels correlated well with C-reactive protein and haptoglobulin, but there was no correlation between IL-1beta or TNF-alpha levels and acute phase proteins such as C-reactive protein, alpha-1 acid glycoprotein, alpha-1 antitrypsin and haptoglobulin.
  
  
  CONCLUSIONS
  These results suggest that serum IL-8 may reflect clinical activity of the disease and may be helpful for monitoring patients with SpA.
authors.affiliation: Kirkpinar sokak, N 25/8, 06540 Cankaya, Ankara, Turkey. bsonel@yahoo.com
authors.forename: Birkan
authors.initials: B
authors.lastname: Sonel
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Tutkak
authors.forename: Nursen
authors.initials: N
authors.lastname: Düzgün
mesh_terms: D000209:Acute-Phase Proteins / Q000032:analysis*; D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood*; D008297:Male; D025242:Spondylarthropathies / Q000097:blood* / Q000276:immunology / Q000503:physiopathology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
chemical_list: D000209:Acute-Phase Proteins; D007375:Interleukin-1; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha