Amyloid binding ligands as Alzheimer's disease therapies.
Extracellular senile plaques (SPs) are hallmark brain lesions of sporadic Alzheimer's disease (AD) and the likely consequence of genetic mutations that cause familial AD by increasing production of amyloidogenic amyloid-beta (Abeta). Although Abeta vaccines and inhibitors of amyloidogenic secretases are potential AD therapies, multifaceted strategies may be needed to effectively interrupt Abeta amyloidosis and prevent/arrest AD. One such strategy is the inhibition of Abeta fibrillization as a potential therapy for AD. Certain amyloid-binding molecules, such as Congo red (CR) and chrysamine G (CG) and Thioflavin S (TS) have been shown to bind SPs with high affinity and they can also arrest the formation of Abeta fibrils; however, CR, CG and TS are unsuitable for AD therapy because they do not cross the blood brain barrier (BBB). Therefore, we have generated novel CG and TS derivatives that specifically recognize fibrillar Abeta in vitro, arrest the formation of Abeta fibrils, and cross the BBB of transgenic (TG) mice that model AD amyloidosis. As proof of their ability to cross the BBB and of their high specificity for Abeta fibrils in vivo, we show that following intravenous injection in TG mice these compounds specifically label AD-like brain deposits of fibrillar Abeta. Furthermore, we demonstrate that CG derivative IMSB binds to SPs comprised of Abeta40 with much higher affinity than Abeta42 whereas TS derivative TDZM shows the opposite affinity. Moreover, IMSB but not TDZM binds selectively to neurofibrillary tangles. Significantly both IMSB and TDZM inhibit Abeta fibrillization in test tubes and in cultured cells. Thus, small amyloid binding molecules such as IMSB and TDZM which cross the BBB are potential therapeutic agents for the treatment of AD.
Lee
Virginia M-Y
VM
The Center for Neurodegenerative Disease Research, Department of Pathology and Laboratory Medicine, HUP, Maloney Bldg., 3rd Floor, University of Pennsylvania School of Medicine, 36th and Spruce Streets, Philadelphia 19104, USA. vmylee@mail.med.upenn.edu
D000544:Alzheimer Disease / Q000188:drug therapy* / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism*; D000686:Amyloidosis / Q000188:drug therapy* / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008024:Ligands; D058225:Plaque, Amyloid / Q000187:drug effects / Q000378:metabolism; D013343:Styrenes / Q000378:metabolism / Q000627:therapeutic use
C430896:1-bromo-2,5-bis(3-hydroxycarbonyl-4-hydroxy)styrylbenzene; C472244:1-iodo-2,5-bis-(3-hydroxycarbonyl-4-methoxy)styryl-benzene; D016229:Amyloid beta-Peptides; D008024:Ligands; D013343:Styrenes