Suspension (S)-FISH, a new technique for interphase nuclei.
We describe a versatile method for performing fluorescence in situ hybridization (FISH) in suspension instead of on a slide as usually done. This so-called suspension-FISH (S-FISH) opens new possibilities for the analysis of shape and functions of the human interphase nucleus. The procedure is described and the first results using this approach are presented.
Steinhaeuser
Ulf
U
Institute of Human Genetics, Jena, Germany.
Starke
Heike
H
Nietzel
Angela
A
Lindenau
Joerg
J
Ullmann
Peter
P
Claussen
Uwe
U
Liehr
Thomas
T
D002467:Cell Nucleus / Q000378:metabolism*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase; D013535:Suspensions
D013535:Suspensions