[Evaluation of povidone abdominal washing for prevention of peritoneal cancer cell seeding: experimental study in the rat].
OBJECTIVE
The aim of the study was to evaluate the in vitro cytototoxicity of diluted povidone iodine on colon cancer cells and its in vivo antitumoral effect in a model of peritoneal carcinomatosis in the rat.


METHODS
Cell cytotoxicity of a povidone iodine diluted solution was assessed, in vitro, on rat colon cancer cells (DHD/K12/PROb) and human colon cancer cells (HT29). The antitumoral effect of diluted povidone iodine washing was measured in BDIX rats after the intraperitoneal inoculation of 10(6) DHD/K12/PROb cells. Results were compared to an abdominal washing within a 9 g/l salinel solution. In one experiment, peritoneal scars and a colocolic anastomosis were performed after the injection of cancer cells.


RESULTS
A short 10 min incubation of human and rat colon cancer cells with diluted povidone iodine resulted in a complete cell killing. In animals, a peritoneal washing with 1% diluted povidone iodine completely inhibited the tumor growth in parietal peritoneum. However, development of peritoneal tumor nodules was not inhibited in the omentum, in scarified peritoneum or in intestinal anastomosis.


CONCLUSIONS
Despite its high in vitro efficacy, diluted povidone iodine has an incomplete effect in the prevention of peritoneal carcinomatosis, with only a partial inhibition in scarred peritoneum epiploïc area and intestinal anastomosis. In contrary, it procures a complete inhibition of tumor growth in normal peritoneum.
Favoulet
P
P
Service de chirurgie digestive, thoracique et cancérologique, Centre Hospitalier Universitaire du Bocage, 1, Avenue du Maréchal de Lattre de Tassigny, 21034 Dijon, France.
Benoit
L
L
Guiu
B
B
Rat
P
P
Chauffert
B
B
Favre
J P
JP
D000818:Animals; D002277:Carcinoma / Q000556:secondary*; D003110:Colonic Neoplasms / Q000473:pathology*; D004195:Disease Models, Animal; D007263:Infusions, Parenteral; D008297:Male; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D009360:Neoplastic Cells, Circulating; D010534:Peritoneal Neoplasms / Q000517:prevention & control* / Q000556:secondary*; D010592:Pharmaceutic Aids / Q000008:administration & dosage / Q000494:pharmacology*; D011205:Povidone / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats
D010592:Pharmaceutic Aids; D011205:Povidone