title: [Regulation of alpha(1) type I collagen promoter by cytokines and losartan].
abstract: OBJECTIVE
  To study the roles of transforming growth factor (TGF) beta(1), PDGF and losartan on regulation of collagen gene promoter.
  
  
  METHODS
  To rat mesangial cells transfected by pCOLH 1.5 and pCOLH 2.5, which were composed of different length of alpha(1) type I collagen gene promoter segments and chloramphenicol acetyltransferase (CAT) were added different concentrations of TGF beta(1), PDGF, Ang II and losartan. The activity of CAT was measured by ELISA technique.
  
  
  RESULTS
  TGF beta(1) enhanced the activity of pCOLH 1.5 and pCOLH 2.5 dose-dependently, showing significant difference with the control (P < 0.01). CAT activities of pCOLH 1.5 and pCOLH 2.5 were not affected with 10 micro g/L and 25 micro g/L of PDGF (P > 0.05). Ang II could increase the CAT activity of the two recombinants. Losartan inhibited the transcriptional activity of the promotor. Losartan + Ang II decreased the enhancement of the activity of promoter recombinant CAT (P < 0.01).
  
  
  CONCLUSIONS
  In mesangial cells, TGF beta(1) enhanced the activity of alpha(1) type I collagen promoter. PDGF did not significantly regulate the activity of CAT. Losartan prevented the increase of the activity of promoter transcription induced by Ang II partly.
authors.affiliation: Department of Nephrology, Navy General Hospital, Beijing 100037, China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Zhou
authors.forename: Changlin
authors.initials: C
authors.lastname: Mei
authors.forename: Chunfang
authors.initials: C
authors.lastname: Gao
authors.forename: Xia
authors.initials: X
authors.lastname: Lei
mesh_terms: D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D024042:Collagen Type I / Q000235:genetics* / Q000378:metabolism; D000090602:Collagen Type I, alpha 1 Chain; D016207:Cytokines / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D019808:Losartan / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D051381:Rats; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D053773:Transforming Growth Factor beta1
chemical_list: D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D016207:Cytokines; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D000804:Angiotensin II; D015500:Chloramphenicol O-Acetyltransferase; D019808:Losartan