Identification of a FasL-like molecule in leucocytes of the teleost fish gilthead seabream (Sparus aurata L.).
The possible expression of FasL in gilthead seabream leucocytes was studied by flow cytometry, immunoblotting and immunocytochemistry, using an anti-mouse FasL monoclonal antibody. The results pointed to a cytosolic FasL-like, but not a membrane-like form, in resting leucocytes from head-kidney, thymus, spleen, blood and peritoneal exudate. Immunoblotting revealed a 19kDa band in resting leucocytes, while activated leucocytes showed the same band and another of 39kDa. The FasL-like molecule is identified in lymphocytes, monocyte-macrophages and acidophilic granulocytes. Phylogenetical and functional implications are suggested.
Cuesta
Alberto
A
Department of Cell Biology, Faculty of Biology, University of Murcia, Campus Universitario de Espinardo, 30100 Murcia, Spain.
Esteban
M Angeles
MA
Meseguer
José
J
D001202:Ascitic Fluid / Q000276:immunology; D053222:Fas Ligand Protein; D005434:Flow Cytometry; D015151:Immunoblotting; D007150:Immunohistochemistry; D007668:Kidney / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000737:chemistry; D008970:Molecular Weight; D021541:Sea Bream / Q000097:blood / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D019014:fas Receptor / Q000032:analysis*
D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008562:Membrane Glycoproteins; D019014:fas Receptor