Prevention of Staphylococcus aureus biofilm on dialysis catheters and adherence to human cells.
BACKGROUND
Dialysis patients, often carriers of Staphylococcus aureus in their nares, are at high risk of S. aureus infections.


METHODS
We examined whether RNAIII inhibiting peptide (RIP), which interferes with quorum sensing mechanisms, reduces adherence of S. aureus to host cells and to dialysis catheter polymers in vitro. Adherence was tested by spectroscopy using safranin staining, by confocal scanning laser microscopy and by atomic force microscopy.


RESULTS
RIP inhibited bacterial adherence to HaCat and HEp-2 cells and reduced adherence and biofilm formation not only on polystyrene, but also on both polyurethane- and silicone-made dialysis catheters, with a preponderant effect on silicone, to which bacteria were more adherent.


CONCLUSIONS
RIP opens a new perspective in anti-S. aureus prophylaxis, particularly in dialysis patients.
Balaban
Naomi
N
Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel. nbalaban@ucdavis.edu
Gov
Yael
Y
Bitler
Arkady
A
Boelaert
Johan R
JR
D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000254:growth & development*; D002408:Catheters, Indwelling / Q000382:microbiology*; D045744:Cell Line, Tumor; D006801:Humans; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000382:microbiology; D007676:Kidney Failure, Chronic / Q000382:microbiology / Q000628:therapy; D009666:Nose / Q000166:cytology; D009842:Oligopeptides / Q000494:pharmacology*; D006435:Renal Dialysis; D012867:Skin / Q000166:cytology; D013203:Staphylococcal Infections / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000254:growth & development*
D009842:Oligopeptides; C111711:RNAIII inhibiting peptide