title: Immunolocalization of inducible nitric oxide synthase in localized juvenile periodontitis patients.
abstract: Localized juvenile periodontitis (LJP) is associated with a destruction of periodontal tissues and the presence of Actinobacillus actinomycetemcomitans (AA). Lipopolysaccharide (LPS) from AA was found to induce a significant macrophage production of nitric oxide (NO). Increased nitric oxide synthase (NOS) activity was found to be negatively correlated with the neutrophil chemotactic response. The aim of this study was to determine the occurrence and distribution of inducible NOS (iNOS) in human gingival tissue from LJP patients. The distribution of iNOS was assessed by monoclonal antibody against iNOS. Cellular markers (CD 3, CD 20, and CD 68) were used to determine the cellular origin of iNOS. The immunostaining revealed the appearance of iNOS in inflamed compared to noninflamed gingival tissues. Macrophages expressed high levels of iNOS that may cause some damage to the periodontal tissues. This study suggests that iNOS activity in macrophages may modify abnormalities of neutrophil function.
authors.affiliation: Department of Oral Medicine and Periodontology, Faculty of Medicine, University of Ljubljana, Hrvatski trg 6, 1000 Ljubljana, Slovenia.
authors.forename: B
authors.initials: B
authors.lastname: Gaspirc
authors.forename: A
authors.initials: A
authors.lastname: Masera
authors.forename: U
authors.initials: U
authors.lastname: Skaleric
mesh_terms: D000293:Adolescent; D010520:Aggressive Periodontitis / Q000201:enzymology*; D005260:Female; D005881:Gingiva / Q000201:enzymology; D006801:Humans; D007150:Immunohistochemistry; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D012016:Reference Values; D014018:Tissue Distribution; D015854:Up-Regulation
chemical_list: C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II