Prodigiosin induces caspase-9 and caspase-8 activation and cytochrome C release in Jurkat T cells.
Prodigiosin (PG) is an active component of bacterial origin, with reported apoptotic effects. We examined the activation of caspases-9, -8, and -3 in PG-treated Jurkat cells in a dose-response study. These caspases were activated in apoptotic cells, as judged by the appearance of cleavage products from procaspases, and cytochrome c (cyt c) was released to the cytosol. In addition, PG induced the cleavage of poly (ADP-ribose) polymerase (PARP). This study demonstrates that the activation of caspases-9, -8, and -3 mediates PG-induced apoptosis.
Montaner
Beatriz
B
Departament de Biologia Cellular i Anatomia Patològica Cancer Cell Biology Research Group, Universitat de Barcelona, Feixa Llarga s/n, E-08907 L'Hospitalet, Barcelona, Spain.
Perez-Tomas
Ricardo
R
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism*; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D007700:Kinetics; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011353:Prodigiosin / Q000494:pharmacology*; D012706:Serratia marcescens
D000900:Anti-Bacterial Agents; D003574:Cytochrome c Group; D011065:Poly(ADP-ribose) Polymerases; C505530:CASP8 protein, human; C506433:CASP9 protein, human; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases; D011353:Prodigiosin