Succinate accumulation in pig large intestine during antibiotic-associated diarrhea and the constitution of succinate-producing flora.
Succinate was the major organic acid detected in the hindgut content of pigs suffering from antibiotic-associated diarrhea. Antibiotic-associated diarrhea was induced by an oral dose of polymyxin B sulfate (3,000,000 units/day) or an intramuscular injection of enrofloxacin (0.6 g enrofloxacin/day). In the large intestine of enrofloxacin-treated pigs, Gram-negative facultative anaerobic rods phylogenetically related to Escherichia coli and Gram-positive facultative anaerobic non-spore-forming rods phylogenetically related to Lactobacilli were isolated as succinate producers. Succinate-producing Lactobacilli were only isolated as the succinate producer in polymyxin B sulfate-treated pigs. In contrast to antibiotic-associated diarrhea pigs, bacteria belonging to Bacteroidaceae, Fusobacteria, and Enterobacteriaceae were detected as succinate producers in a non-treated pig. In antibiotic-associated diarrhea conditions, antibiotic-resistant Enterobacteria, E. coli in particular, and Lactobacilli may contribute to an abnormal succinate accumulation and may affect water absorption in the hindgut that relates to an expression of antibiotic-associated diarrhea.
Tsukahara
Takamitsu
T
Laboratory of Animal Science, Kyoto Prefectural University, Japan.
Ushida
Kazunari
K
D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D000890:Anti-Infective Agents / Q000633:toxicity; D015169:Colony Count, Microbial; D003967:Diarrhea / Q000139:chemically induced / Q000378:metabolism*; D000077422:Enrofloxacin; D004755:Enterobacteriaceae / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D024841:Fluoroquinolones; D006863:Hydrogen-Ion Concentration; D007420:Intestine, Large / Q000378:metabolism* / Q000382:microbiology*; D007777:Lactobacillaceae / Q000378:metabolism*; D008297:Male; D015363:Quinolones / Q000633:toxicity; D019802:Succinic Acid / Q000378:metabolism*; D013552:Swine
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D005227:Fatty Acids; D024841:Fluoroquinolones; D015363:Quinolones; D000077422:Enrofloxacin; D019802:Succinic Acid