title: Dma1 prevents mitotic exit and cytokinesis by inhibiting the septation initiation network (SIN).
abstract: In the fission yeast Schizosaccharomyces pombe, the septation initiation network (SIN) triggers cytokinesis after mitosis. We investigated the relationship between Dma1p, a spindle checkpoint protein and cytokinesis inhibitor, and the SIN. Deletion of dma1 inactivates the spindle checkpoint and allows precocious SIN activation, while overexpressing Dma1p reduces SIN signaling. Dma1p seems to function by inhibiting the SIN activator, Plo1p kinase, since dma1 overexpression and deletion phenotypes suggest that Dma1p antagonizes Plo1p localization. Furthermore, failure to maintain high cyclin-dependent kinase (CDK) activity during spindle checkpoint activation in dma1 deletion cells requires Plo1p. Dma1p itself localizes to spindle pole bodies through interaction with Sid4p. Our observations suggest that Dma1p functions to prevent mitotic exit and cytokinesis during spindle checkpoint arrest by inhibiting SIN signaling.
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01605, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Guertin
authors.forename: Srinivas
authors.initials: S
authors.lastname: Venkatram
authors.forename: Kathleen L
authors.initials: KL
authors.lastname: Gould
authors.forename: Dannel
authors.initials: D
authors.lastname: McCollum
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division / Q000235:genetics; D029721:Drosophila Proteins; D018816:Genes, cdc / Q000502:physiology*; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D008938:Mitosis / Q000235:genetics*; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics; D012568:Schizosaccharomyces / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins; D015398:Signal Transduction / Q000235:genetics; D008941:Spindle Apparatus / Q000235:genetics* / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; C103134:Dma1 protein, S pombe; D029721:Drosophila Proteins; D008869:Microtubule-Associated Proteins; D029702:Schizosaccharomyces pombe Proteins; C409024:Sid4 protein, S pombe; D017346:Protein Serine-Threonine Kinases