title: Modulation by polyamines of gamma-glutamyl transpeptidase activity and lactate production in cultured Sertoli cells from immature and adult regressed golden hamster.
abstract: Polyamines are involved in cellular growth and differentiation. To analyze a possible role of polyamines on the regulation of Sertoli cell function, we studied the effect of putrescine, spermidine, and spermine on gamma-glutamyl transpeptidase (gamma-GTP) activity and lactate production on Sertoli cell cultures obtained from immature and adult-regressed golden hamsters. Sertoli cells were cultured for 7 days. The 72 hour conditioned media obtained on day 6 were used to evaluate lactate levels. Gamma glutamyl transpeptidase activity was determined in the cells harvested on day 7. Cultured Sertoli cells isolated from immature and adult-regressed golden hamsters exhibited a clear morphological response to follicle-stimulating hormone (FSH) and to spermine. Gamma glutamyl transpeptidase activity increased in response to FSH in a dose-dependent manner. Dose-dependent stimulation of lactate production by FSH was also observed. For each functional parameter, a similar ED50 value of FSH stimulation was observed in both groups of animals. Spermine increased basal and FSH-stimulated gamma-GTP activity in immature and adult-regressed Sertoli cell cultures. A stimulatory effect of spermidine and putrescine on gamma-GTP activity was exclusively observed in adult-regressed Sertoli cell cultures. In Sertoli cells obtained from immature hamsters, spermine exerted a stimulatory effect on basal and FSH-stimulated lactate production. These results suggest that, in addition to the known effects of hormones and paracrine factors, polyamines may influence the functionality of Sertoli cells.
authors.affiliation: Centro de Investigaciones Endocrinológicas, Hospital de Niños R. Gutiérrez, Universidad de Buenos Aires, Argentina.
authors.forename: H F
authors.initials: HF
authors.lastname: Schteingart
authors.forename: S B
authors.initials: SB
authors.lastname: Cigorraga
authors.forename: R S
authors.initials: RS
authors.lastname: Calandra
authors.forename: S I
authors.initials: SI
authors.lastname: Gonzalez-Calvar
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae / Q000502:physiology*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D019344:Lactic Acid / Q000096:biosynthesis*; D008297:Male; D017440:Photoperiod; D011700:Putrescine / Q000494:pharmacology*; D012098:Reproduction / Q000528:radiation effects; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D013095:Spermidine / Q000494:pharmacology*; D013096:Spermine / Q000494:pharmacology*; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
chemical_list: D013096:Spermine; D019344:Lactic Acid; D005640:Follicle Stimulating Hormone; D005723:gamma-Glutamyltransferase; D013095:Spermidine; D011700:Putrescine