Monitoring of active HHV-6 infection in bone marrow transplant recipients by real time PCR; comparison to detection of viral DNA in plasma by qualitative PCR.
Twelve (46%) of the 26 patients had human herpesvirus 6 (HHV-6) viremia after bone marrow transplant (BMT). All isolates were recovered from the samples obtained at 2 weeks after BMT. The sensitivity and the specificity of detection of viral DNA in plasma by qualitative polymerase chain reaction (PCR) for monitoring active virus replication were 92% and 97% respectively. Moreover, the positive (85%) and negative (99%) predictive values were also high. The patients with HHV-6 viremia showed a clear peak in HHV-6 DNA in peripheral blood mononuclear cells (PBMCs) at 2 weeks after BMT, which was measured by real time PCR. The virus DNA level in PBMCs between the two groups (patients with viremia and patients without viremia) was statistically different at 2 weeks after BMT (P = 0.033). In patients with HHV-6 viremia, mean HHV-6 DNA copy number was higher in the samples collected at 2 weeks after BMT than the samples collected at any other time period.
Ihira
Masaru
M
Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
Yoshikawa
Tetsushi
T
Suzuki
Kyoko
K
Ohashi
Masahiro
M
Suga
Sadao
S
Horibe
Keizo
K
Tanaka
Naoko
N
Kimura
Hiroshi
H
Kojima
Seiji
S
Kato
Koji
K
Matsuyama
Takaharu
T
Nishiyama
Yukihiro
Y
Asano
Yoshizo
Y
D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D004279:DNA, Viral / Q000097:blood* / Q000737:chemistry / Q000235:genetics; D005260:Female; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016867:Immunocompromised Host; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D019349:Roseolovirus Infections / Q000097:blood / Q000821:virology*; D012680:Sensitivity and Specificity; D014766:Viremia / Q000175:diagnosis
D004279:DNA, Viral