Isolation and characterization of the gene URA3 encoding the orotidine-5'-phosphate decarboxylase from Torulaspora delbrueckii.
A DNA fragment containing the URA3 gene from Torulaspora delbrueckii was isolated by complementation cloning in Saccharomyces cerevisiae. DNA sequence analysis revealed the presence of an ORF of 795 bp, encoding a 264 amino acid protein, which shares a high similarity to the Saccharomycetaceae Ura3 proteins. Furthermore, the cloned ORF fully complemented the ura3 mutation of S. cerevisiae, confirming that it encodes for the TdUra3 protein. The GeneBank Accession No. for TdURA3 is AF518402.
Hernandez-Lopez
Maria Jose
MJ
Department of Biotechnology, Instituto de Agroquímica y Tecnología de los Alimentos, Consejo Superior de Investigaciones Científicas, PO Box 73, 46100-Burjassot, Valencia, Spain.
Prieto
Jose Antonio
JA
Randez-Gil
Francisca
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D015003:Yeasts / Q000201:enzymology / Q000235:genetics*
D005656:Fungal Proteins; D009964:Orotidine-5'-Phosphate Decarboxylase