[Allelic analysis on chromosome 5 in sporadic colorectal cancer patients].
OBJECTIVE
To evaluate and map the putative tumor suppressor loci on chromosome 5 involved in tumor progress or metastasis.


METHODS
Chromosome 5 of 83 patients with sporadic colorectal cancer was systemically screened. Fifteen microsatellite marker primers labeled with 3 different fluorescents were used to amplify the corresponding loci of the genome DNA. The PCR products were electrophoresed on a 377 PRISM sequencer and the fluorescent signals were analyzed with Genotyper and Genescan software.


RESULTS
The highest loss of heterozygosity (LOH) ratio was found at D5S416 (48.15%) on 5p and at D5S471 (38.71%) on 5q. The region (5q13.3 - 31), where D5S471 and 3 neighboring loci (D5S428, D5S2027 and D5S2115) reside, presented high frequent LOH.


CONCLUSIONS
The deletion of APC, MCC, CTNNA1 and IL cluster in the 5q 13.3 - 31.1 area play important role in the tumorogenesis of colorectal cancer, and the expected existence of another novel tumor suppressor gene on 5p is possible.
Zhang
Fang
F
Department of General Surgery, First People's Hospital, Shanghai 200080, China.
Zhou
Chongzhi
C
Ling
Yun
Y
Qiu
Guoqiang
G
Bai
Shaochun
S
Liu
Wanqing
W
He
Lin
L
Peng
Zhihai
Z
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D002872:Chromosome Deletion; D002895:Chromosomes, Human, Pair 5; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged