Simultaneous demonstration of acid phosphatase and glucose-6-phosphate dehydrogenase in mouse hepatocytes. A novel electron-microscopic dual staining enzyme-cytochemistry.
Acid phosphatase (ACPase) and glucose-6-phosphate dehydrogenase (G6PD) play important roles in cell biology/disease pathophysiology in various organs including the liver. The purpose of the present report is to introduce a new enzymecytochemical method to simultaneously demonstrate the subcellular localization of ACPase and G6PD within the same hepatocyte in the mouse liver. The ultrastructural localization of ACPase and G6PD were demonstrated, with concomitant use of the cerium method and the copper-ferrocyanide method, respectively. ACPase labelings were localized in the lysosomes, and G6PD labelings were visible in the cytoplasm and on the cytosolic side of the endoplasmic reticulum of the hepatocyte. This novel double staining procedure may be a useful histochemical tool for the study of liver functions in both physiological and pathological conditions.
Matsubara
S
S
Department of Obstetrics, Gynecology and Anatomy, Jichi Medical School, Minamikawachi-machi 3311-1, Kawachi-gun, Tochigi 329-0498, Japan. matsushi@jichi.ac.jp
D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D002563:Cerium; D004396:Coloring Agents; D003593:Cytoplasm / Q000201:enzymology / Q000648:ultrastructure; D003600:Cytosol / Q000201:enzymology / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000648:ultrastructure; D005295:Ferrocyanides; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D022781:Hepatocytes / Q000201:enzymology* / Q000648:ultrastructure; D006651:Histocytochemistry; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron; D013194:Staining and Labeling; D013347:Subcellular Fractions
D004396:Coloring Agents; D005295:Ferrocyanides; D002563:Cerium; C039105:cupric ferrocyanide; D005954:Glucosephosphate Dehydrogenase; D000135:Acid Phosphatase