title: VCIP135, a novel essential factor for p97/p47-mediated membrane fusion, is required for Golgi and ER assembly in vivo.
abstract: NSF and p97 are ATPases required for the heterotypic fusion of transport vesicles with their target membranes and the homotypic fusion of organelles. NSF uses ATP hydrolysis to dissociate NSF/SNAPs/SNAREs complexes, separating the v- and t-SNAREs, which are then primed for subsequent rounds of fusion. In contrast, p97 does not dissociate the p97/p47/SNARE complex even in the presence of ATP. Now we have identified a novel essential factor for p97/p47-mediated membrane fusion, named VCIP135 (valosin-containing protein [VCP][p97]/p47 complex-interacting protein, p135), and show that it binds to the p97/p47/syntaxin5 complex and dissociates it via p97 catalyzed ATP hydrolysis. In living cells, VCIP135 and p47 are shown to function in Golgi and ER assembly.
authors.affiliation: Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, United Kingdom.
authors.forename: Keiji
authors.initials: K
authors.lastname: Uchiyama
authors.forename: Eija
authors.initials: E
authors.lastname: Jokitalo
authors.forename: Fumi
authors.initials: F
authors.lastname: Kano
authors.forename: Masayuki
authors.initials: M
authors.lastname: Murata
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.forename: Benito
authors.initials: B
authors.lastname: Canas
authors.forename: Richard
authors.initials: R
authors.lastname: Newman
authors.forename: Catherine
authors.initials: C
authors.lastname: Rabouille
authors.forename: Darryl
authors.initials: D
authors.lastname: Pappin
authors.forename: Paul
authors.initials: P
authors.lastname: Freemont
authors.forename: Hisao
authors.initials: H
authors.lastname: Kondo
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D015415:Biomarkers / Q000032:analysis; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000502:physiology*; D002460:Cell Line; D002478:Cells, Cultured; D017124:Conserved Sequence; D006224:Cricetinae; D003600:Cytosol / Q000201:enzymology / Q000378:metabolism; D053487:DEAD-box RNA Helicases; D010450:Endopeptidases; D004721:Endoplasmic Reticulum / Q000502:physiology*; D006056:Golgi Apparatus / Q000502:physiology*; D006868:Hydrolysis; D036341:Intercellular Signaling Peptides and Proteins; D008099:Liver / Q000166:cytology; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D008954:Models, Biological; D009687:Nuclear Proteins / Q000737:chemistry / Q000502:physiology*; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D051381:Rats; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D000074405:Valosin Containing Protein
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D010455:Peptides; C046951:valosin; C075446:Ddx39b protein, rat; D010450:Endopeptidases; C470473:Vcpip1 protein, rat; D000251:Adenosine Triphosphatases; D053487:DEAD-box RNA Helicases; D000074405:Valosin Containing Protein; C000614180:Vcp protein, rat