title: Inducible nitric oxide synthase knockout mouse and low-density lipoprotein oxidation.
abstract: We have presented experimental procedures that examine macrophage-mediated LDL oxidation using Ham's F-10 medium. By comparing iNOS-/- and iNOS+/+ macrophages, an antioxidant effect for NO and a prooxidant effect for IFN-gamma were demonstrated. The methods outlined here should allow for the investigation on the mechanism of in vitro LDL oxidation and how the macrophage-mediated LDL oxidation process is affected by various factors, one of which was the effect of iNOS induction by IFN-gamma.
authors.affiliation: Department of Medicine, Duke University Medical Center, Durham, North Carolina 27705, USA.
authors.forename: Xi-Lin
authors.initials: XL
authors.lastname: Niu
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Shoyama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ishiwata
authors.forename: Ruriko
authors.initials: R
authors.lastname: Obama
authors.forename: Hiroe
authors.initials: H
authors.lastname: Nakazawa
mesh_terms: D000818:Animals; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000502:physiology*; D052247:Nitric Oxide Synthase Type II; D010084:Oxidation-Reduction
chemical_list: D008077:Lipoproteins, LDL; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse