External Ca2+ is predominantly used for cytoplasmic and nuclear Ca2+ increases in fertilized oocytes of the marine bivalve Mactra chinensis.
Oocytes of the marine bivalve Mactra chinensis are spawned and arrested at the germinal vesicle stage (first meiotic prophase) until fertilization, without undergoing a process called oocyte maturation. As is the case of other animals, a fertilized oocyte of the bivalve displays increases in intracellular free Ca(2+). We have clarified here the spatiotemporal patterns and sources of the intracellular Ca(2+) changes at fertilization. Shortly after insemination, increased Ca(2+) simultaneously appeared at the whole cortical region of the oocyte and spread inwardly to the center, attaining the maximal Ca(2+) levels throughout the oocyte, including the cytoplasm and nucleus. The initial maximal Ca(2+) peak was followed by a submaximal plateau phase of cytoplasmic and nuclear Ca(2+) elevations, which persisted for several minutes. The nuclear envelope began to break down shortly before the termination of the plateau phase. These sperm-induced Ca(2+) changes were inhibited by suppression of the influx of external Ca(2+) from seawater but not by disturbance of the release of internal Ca(2+) from inositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)]-sensitive stores, suggesting that the increased Ca(2+) is from an external source. In contrast to the situation observed at fertilization, an oocyte artificially stimulated with serotonin (5-hydroxytryptamine, 5-HT) displayed repetitive Ca(2+) transients, each of which started from one cortical region and propagated across the oocyte as a Ca(2+) wave. The 5-HT-induced Ca(2+) transients persisted even in the absence of external Ca(2+). Experiments with caged Ins(1,4,5)P(3) revealed that Ca(2+) release from Ins(1,4,5)P(3)-sensitive stores is another pathway that is sufficient to trigger meiosis reinitiation from the first prophase. These results demonstrate that Mactra oocytes can potentially use two different Ca(2+)-mobilizing pathways: Ca(2+) influx producing a centripetal Ca(2+) wave from the whole cortex and Ca(2+) release from Ins(1,4,5)P(3)-sensitive stores producing a point-source propagating Ca(2+) wave. However, it seems likely that the Ca(2+) influx pathway is predominantly activated at fertilization.
Deguchi
Ryusaku
R
Department of Biology, Miyagi University of Education, Aoba-ku, Sendai, Miyagi 980-0845, Japan. deguchi@staff.miyakyo-u.ac.jp
Morisawa
Masaaki
M
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005306:Fertilization / Q000502:physiology; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D008974:Mollusca / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D012701:Serotonin; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium