Direct cloning of full-length cell differentially expressed genes by multiple rounds of subtractive hybridization based on long-distance PCR and magnetic beads.
Huang
Xin
X
Department of Immunology, Second Military Medical University, Shanghai, China.
Yuan
Zhenglong
Z
Cao
Xuetao
X
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D007202:Indicators and Reagents; D008280:Magnetics; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods; D013698:Templates, Genetic
D017931:DNA Primers; D018076:DNA, Complementary; D007202:Indicators and Reagents; D012333:RNA, Messenger