[Preimplantation genetic diagnosis].
OBJECTIVE
To evaluate the clinical effectiveness of preimplantation genetic diagnosis (PGD) for the couples having a higher risk of genetic diseases with fluorescence in situ hybridization (FISH).


METHODS
Ovarian hyperstimulation, oocyte retrieval and in vitro fertilization were performed in 4 cases. Blastomere biopsy were performed from the embryos at the 6 - 8 cell stage. Genetic diagnosis was identified by single blastomere FISH. The female embryos or normal embryos were transferred into uterus.


RESULTS
Twelve embryos were available for embryo biopsy. Twenty cells were collected. Seventeen nuclei were found after fixation. Hybridization signals of 11 embryos were clear and 8 embryos were transferred into uterus. One clinical pregnancy was achieved, and 1 normal female infant was delivered on september 14, 2001.


CONCLUSIONS
PGD is very valuable for avoiding selective abortion and birth of affected child of the couples having a higher risk of genetic diseases by detecting embryo sex and chromosome number using FISH.
Meng
Xiangge
X
Reproductive Medical Research Center, Shandong Institute for Family Planning, Jinan 250002, China.
Zhang
Lihong
L
Dong
Yunling
Y
Li
Juan
J
Wang
Kehua
K
Jiang
Ping
P
Zhnag
Meixin
M
Xu
Yumei
Y
D000328:Adult; D001706:Biopsy; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D011247:Pregnancy; D019836:Preimplantation Diagnosis