title: Fruit-juice concentrate of Asian plum inhibits growth signals of vascular smooth muscle cells induced by angiotensin II.
abstract: Bainiku-ekisu, the fruit-juice concentrate of the Oriental plum (Prunus mume) has recently been shown to improve human blood fluidity. We have shown that angiotensin II (AngII) stimulates growth of vascular smooth muscle cells (VSMCs) through epidermal growth factor (EGF) receptor transactivation that involves reactive oxygen species (ROS) production. To better understanding the possible cardiovascular protective effect of Bainiku-ekisu, we have studied whether Bainiku-ekisu inhibits AngII-induced growth promoting signals in VSMCs. Bainiku-ekisu markedly inhibited AngII-induced EGF receptor transactivation. H(2)O(2)-induced EGF receptor transactivation was also inhibited by Bainiku-ekisu. Thus, Bainiku-ekisu markedly inhibited AngII-induced extracellular signal-regulated kinase (ERK) activation. However, EGF-induced ERK activation was not affected by Bainiku-ekisu. AngII stimulated leucine uptake in VSMCs that was significantly inhibited by Bainiku-ekisu. Also, Bainiku-ekisu possesses a potent antioxidant activity. Since the activation of EGF receptor, ERK and the production of ROS play central roles in mediating AngII-induced vascular remodeling, these data suggest that Bainiku-ekisu could exert a powerful cardiovascular protective effect with regard to cardiovascular diseases.
authors.affiliation: Department of Pathology, Wakayama Medical University, Japan.
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Utsunomiya
authors.forename: Susumu
authors.initials: S
authors.lastname: Takekoshi
authors.forename: Nobuki
authors.initials: N
authors.lastname: Gato
authors.forename: Hisao
authors.initials: H
authors.lastname: Utatsu
authors.forename: Evangeline D
authors.initials: ED
authors.lastname: Motley
authors.forename: Kunie
authors.initials: K
authors.lastname: Eguchi
authors.forename: Trinita G
authors.initials: TG
authors.lastname: Fitzgerald
authors.forename: Mizuo
authors.initials: M
authors.lastname: Mifune
authors.forename: Gerald D
authors.initials: GD
authors.lastname: Frank
authors.forename: Satoru
authors.initials: S
authors.lastname: Eguchi
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D019343:Citric Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D066246:ErbB Receptors / Q000037:antagonists & inhibitors / Q000378:metabolism; D005663:Furans / Q000302:isolation & purification / Q000494:pharmacology*; D015227:Lipid Peroxidation; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D014176:Protein Biosynthesis; D027861:Prunus; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D015398:Signal Transduction; D015533:Transcriptional Activation
chemical_list: D005663:Furans; D010936:Plant Extracts; C412144:mumefural; D000804:Angiotensin II; D019343:Citric Acid; D066246:ErbB Receptors; D020928:Mitogen-Activated Protein Kinases; D002118:Calcium