Maximizing production of Penicillium cyclopium partial acylglycerol lipase.
Penicillium cyclopium partial acylglycerol lipase production was maximized in shaken batch culture. The effect of inoculum size and substrate concentration on the lipase activity released in the culture medium was visualized using a surface response methodology based on a Doehlert experimental design. The main advantage of this approach is the low number of experiments required to construct a predictive model of the experimental domain. Substrate percentage (corn steep, w/v) ranged from 0.1% to 1.9% and inoculum from 100 spores/ml to 3,200 spores/ml. We determined that an optimal set of experimental conditions for high lipase production was 1.0% substrate and 3,200 spores/ml, with initial pH 5.0, temperature 25 degrees C and shaking speed 120 rpm. Between the conditions giving the minimum and the maximum lipase production, we observed a three-fold increase in both the predicted and the measured values.
Vanot
G
G
Laboratoire de Chimie Biologique Appliquée, UDESAM, Faculté des Sciences et Techniques de St-Jérôme, Service 431, Avenue Escadrille Normandie-Niemen, 13397 Marseille Cedex 20, France. guillaume.vanot@univ.u-3mrs.fr
Valérie
D
D
Guilhem
M-C
MC
Phan Tan Luu
R
R
Comeau
L-C
LC
D001709:Biotechnology; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000096:biosynthesis* / Q000378:metabolism; D008954:Models, Biological; D008994:Monoacylglycerol Lipases / Q000096:biosynthesis* / Q000378:metabolism; D009716:Numerical Analysis, Computer-Assisted; D010407:Penicillium / Q000201:enzymology* / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
D003470:Culture Media; D008994:Monoacylglycerol Lipases; D008049:Lipase