Determination of available phenolic compounds in soils by liquid chromatography with solid-phase extraction.
A fast, selective, and sensitive liquid chromatographic (LC) method was developed for determination of derivatives of benzoic and cinnamic acids (gallic, protocatechuic, 2,3,4-trihydroxybenzoic, 4-hydroxybenzoic, vanillic, caffeic, syringic, 4-coumaric, ferulic, sinapic, benzoic, 2-coumaric, cinnamic acids, and 4-hydroxybenzaldehyde and vanillin) in soil samples. The method for sample pretreatment is based on temperature-controlled extraction with water (pH 5.6) for 60 min. Extracts were preconcentrated and purified by solid-phase extraction on OASIS HLB sorbent, with subsequent separation and quantification of individual substances by LC with UV diode-array detection. Limits of detection (3 signal-to-noise LODs) better than 65 ng/g (dry weight) and recoveries from 88 to 99% were found for each compound at absorbance 280 nm. The method was used for determination of bioavailable phenolic compounds in different soil samples.
Matejícek
David
D
Mendel University of Agriculture and Forestry, Department of Chemistry and Biochemistry, Brno, Czech Republic.
Klejdus
Borivoj
B
Kubán
Vlastimil
V
D002853:Chromatography, Liquid; D005612:Freeze Drying; D006851:Hydrochloric Acid; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D010636:Phenols / Q000032:analysis*; D012015:Reference Standards; D012987:Soil / Q000032:analysis*; D012997:Solvents
D007202:Indicators and Reagents; D010636:Phenols; D012987:Soil; D012997:Solvents; D006851:Hydrochloric Acid