In vitro anti-Neisseria gonorrhoeae activity of Terminalia macroptera leaves.
We used the agar dilution method to evaluate the antibacterial effect of Terminalia macroptera leaf (Tml) extract against nine reference and clinical Neisseria gonorrhoeae strains, including penicillin- and tetracycline-resistant and -susceptible strains. Tml possesses anti-N. gonorrhoeae activity against all of the strains and the minimum inhibitory concentrations (MIC) were between 100 and 200 microg ml(-1). We then used a liquid-liquid partition method to divide the Tml extract into five fractions and determined the anti-N. gonorrhoeae activity of each of the fractions. All of the fractions showed antibacterial activity. The most active one was identified as the diethyl ether fraction and had MIC values of between 25 and 50 microg ml(-1) against all of the strains.
Silva
Olga
O
CECF, Laboratory of Pharmacognosy, Faculty of Pharmacy, University of Lisbon, Av das Forças Armadas, 1649-019 Lisbon, Portugal.
Ferreira
Eugénia
E
Vaz Pato
M
M
Caniça
Manuela
M
Gomes
Elsa T
ET
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008263:Macrophage Migration-Inhibitory Factors; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D029766:Terminalia / Q000737:chemistry* / Q000145:classification / Q000378:metabolism
D000900:Anti-Bacterial Agents; D008263:Macrophage Migration-Inhibitory Factors; D010936:Plant Extracts