Distribution of nociceptin-like immunoreactivity in the central nervous system of the Mongolian gerbil: an immunohistochemical study.
This study is designed to demonstrate the distribution of nociceptin, endogenous ORL1 receptor ligand, in the central nervous system of the Mongolian gerbil. To intensify the nociceptin-like immunoreactivity (NOC-LI), colchicine was administered into the lateral ventricle, at 48 h prior to the transcardiac perfusion. In the group without colchicine treatment, NOC-LI was observed in the fibres of the spinal dorsal horn, specifically in the superficial layers. However, the NOC-LI in the superficial layers disappeared after the administration of colchicine. In the brain, NOC-LI was prominent in the hypothalamus, hippocampus, cerebral peduncle, substantia nigra, dorsal raphe, periaqueductal grey, locus coeruleus and trapezoid nucleus. Colchicine treatment markedly intensified the NOC-LI in the somata of the central nervous system, whereas the untreated sections were too weak to observe and analyse. The distribution of NOC-LI provides informative data for studies of the neuronal circuit that nociceptin may be involved in.
Kim
H W
HW
Kwon
Y B
YB
Ham
T W
TW
Roh
D H
DH
Yoon
S Y
SY
Han
H J
HJ
Kim
K W
KW
Lee
W S
WS
Kim
J K
JK
Yoon
O B
OB
Lee
J H
JH
D000818:Animals; D001921:Brain / Q000378:metabolism / Q000502:physiology; D002490:Central Nervous System / Q000378:metabolism*; D003078:Colchicine / Q000494:pharmacology; D005849:Gerbillinae / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D009619:Nociceptors / Q000378:metabolism*; D018847:Opioid Peptides / Q000276:immunology / Q000378:metabolism*; D000097629:Nociceptin
D003078:Colchicine; D018847:Opioid Peptides; D000097629:Nociceptin