title: Pituitary adenylate-cyclase-activating polypeptide expression in the immune system.
abstract: Although pituitary adenylate-cyclase-activating polypeptide (PACAP) is a multifunctional and pleiotropic neuropeptide with many different immunomodulatory properties, investigations of its source in lymphoid organs are scarce. The present report contributes to the knowledge on the origin and synthesis of this peptide in immune cells of the lymphoid organs and peritoneum using immunohistochemistry, immunocytochemical staining, Western blot and RT-PCR methods. Our study reveals PACAP immunoreactivity in the thymus, spleen and lymph nodes. Cytochemical results show that PACAP is present in thymocytes, lymphocytes and plasma cells from the spleen and lymph nodes. Western blot analysis showed a band corresponding to PACAP for all lymphoid organs studied. mRNA appears in both double (CD4+CD8+)- and single-positive (CD4+CD8-, CD4-CD8+) thymocytes and in T subsets and B cells from the spleen and lymph nodes. In addition, PACAP mRNA is expressed in lymphocytes, but not in macrophages from peritoneal suspensions. Our findings show that PACAP produced by lymphocytes could be added to the growing list of mediators shared by nervous, endocrine and immune systems. Moreover, PACAP could be considered as a lymphocyte-derived cytokine in the central and peripheral lymphoid organs acting on lymphocytes and stromal cells.
authors.affiliation: Department of Cellular Biology, Faculty of Biology, Complutense University, Madrid, Spain.
authors.forename: Catalina
authors.initials: C
authors.lastname: Abad
authors.forename: Carmen
authors.initials: C
authors.lastname: Martinez
authors.forename: Javier
authors.initials: J
authors.lastname: Leceta
authors.forename: Maria G
authors.initials: MG
authors.lastname: Juarranz
authors.forename: Mario
authors.initials: M
authors.lastname: Delgado
authors.forename: Rosa P
authors.initials: RP
authors.lastname: Gomariz
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D015704:CD4 Antigens / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D005784:Gene Amplification / Q000235:genetics; D007107:Immune System / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D009479:Neuropeptides / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D010950:Plasma Cells / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology / Q000378:metabolism
chemical_list: C495403:Adcyap1 protein, rat; D015704:CD4 Antigens; D016827:CD8 Antigens; D016207:Cytokines; D018076:DNA, Complementary; D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D012333:RNA, Messenger