Study of isolation of fluoroquinolone-resistant Ureaplasma urealyticum and identification of mutant sites.
OBJECTIVE
To study the resistance mechanism of clinical isolates of Ureaplasma urealyticum resistant to fluoroquinolones.


METHODS
Thirteen isolates of Ureaplasma urealyticum resistant to six fluoroquinolones were selected out of 184 clinical isolates and their QRDRs (quinolone resistance-determining region) gyrA, gyrB, parC and parE were amplified by PCR. Sequencing results were compared to those susceptible reference strains and a comparison of deduced amino acid sequences were performed.


RESULTS
Sequence comparison revealed a C to A change at 87nt of gyrA QRDR leading to the substitution of Asp95 with glutamic acid and a C to T change at 50nt of parC QRDR leading to the substitution of Ser80 with leucine.


CONCLUSIONS
These results suggest that a C to A change at 87nt of gyrA QRDR and a C to T change at 50nt of parC QRDR are associated with fluoroquinolone resistance of Ureaplasma urealyticum.
Zhang
Wenbo
W
Department of Microbiology, Basic Medical College, Nanhua University, Hengyang 421001, Hunan Province, China.
Wu
Yimou
Y
Yin
Weiguo
W
Yu
Minjun
M
D019943:Amino Acid Substitution; D000890:Anti-Infective Agents / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D024841:Fluoroquinolones; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D016990:Ureaplasma urealyticum / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase