Design and fabrication of a silica on silicon integrated optical biochip as a fluorescence microarray platform.
Previous research into the use of Flame Hydrolysis Deposition (FHD) of glasses in integrated optics has focused on the successful commercial exploitation of low cost optical devices within the field of telecommunications and optoelectronics. Recently we have sought to apply these fabrication technologies to the development of optical biochips, utilising their ability to be integrated with microfluidics as a 'Lab-on-a-chip' platform. In this paper, we carry this development forward by seeking to create a microarray of integrated optical sensing elements, addressed using a glass-polymer hybrid technology in which poly(dimethylsiloxane), PDMS, is used as an elastomeric packaging over-layer. In particular, we describe the wide range of modelling and microfabrication processes required for the successful manufacture, integration and packaging of such arrays. The integration of both optical and fluidic circuits in this device avoids precise alignment requirements and results in a compact, robust and reliable device. Finally, in this paper, we describe the implementation of a pumping system for delivering small amounts of fluid across the array together with an optical signal treatment.
Ruano
Jesus M
JM
Department of Electronics and Electrical Engineering, The University of Glasgow, Glasgow G12 8LT, UK.
Glidle
Andrew
A
Cleary
Alison
A
Walmsley
Adrian
A
Aitchison
J Stewart
JS
Cooper
Jonathan M
JM
D002232:Carbocyanines / Q000032:analysis; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D020650:Combinatorial Chemistry Techniques / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D005470:Fluorometry / Q000295:instrumentation* / Q000379:methods; D008567:Membranes, Artificial; D008832:Microchemistry / Q000295:instrumentation; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D055096:Optical Devices; D055095:Optics and Photonics / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012825:Silicon
D002232:Carbocyanines; D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; C085321:cyanine dye 5; D012825:Silicon