title: Efficient germline transmission of mouse embryonic stem cells grown in synthetic serum in the absence of a fibroblast feeder layer.
authors.affiliation: Immunology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, United Kingdom. wardcm@hotmail.com
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Ward
authors.forename: Peter
authors.initials: P
authors.lastname: Stern
authors.forename: Mark A
authors.initials: MA
authors.lastname: Willington
authors.forename: Ann Marie
authors.initials: AM
authors.lastname: Flenniken
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002678:Chimera; D003470:Culture Media; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D005796:Genes; D005854:Germ Cells / Q000166:cytology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology
chemical_list: D003470:Culture Media