[Study of the mutation and expression of PTEN gene in endometrial carcinoma and epithelial ovarian cancer].
OBJECTIVE
To study the relationship between the mutation and protein expression of PTEN and carcinogenesis of endometrial carcinoma and epithelial ovarian cancer.


METHODS
The mutations of Exon5, 8 of PTEN in epithelial ovarian cancer tissues and endometrial carcinoma tissues were examined by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequence analysis; and immunohistochemistry was used to evaluate the expression of PTEN protein in corresponding tissues.


RESULTS
The mutation rate (25%) in endometrial carcinomas was higher than that of normal tissue (0, P < 0.05) and the mutation rate had significant relationship with pathological grade, histological type and depth of myometrial invasion (P < 0.05). PTEN protein expression deletion rate (60%) in endometrial carcinoma tissues was higher than that of normal tissue (0, P < 0.05). The protein expression deletion was significantly associated with pathological grade and histological type (P < 0.05), but was irrelevant to stage and depth of myometrial invasion (P > 0.05). The mutation frequency in epithelial ovarian carcinomas (5%) had no significant difference compared with those of normal ovarian tissues (0) and benign epithelial ovarian tumors (0). PTEN protein expression deletion in epithelial ovarian cancers (23%) was higher than those of normal ovarian tissues (0) and benign epithelial ovarian tumors (0, P < 0.05). The expression level of PTEN protein varied between different stages and differential grades. There was no difference between the expression levels of two different histological types and different ages.


CONCLUSIONS
The mutation and expression deletion of PTEN played a role in the carcinogenesis of endometrial cancers, however, in epithelial ovarian cancers PTEN gene played its role by expression deletion other than mutation.
Che
Yanci
Y
Department of Obstetrics and Gynecology, Affiliated Hospital of Medical College of Qingdao University, Qingdao 266003,China.
Yao
Qin
Q
Dai
Shuzhen
S
Luo
Bing
B
Wang
Yankui
Y
D000328:Adult; D000368:Aged; D016889:Endometrial Neoplasms / Q000235:genetics*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics*; D051059:PTEN Phosphohydrolase; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000032:analysis / Q000235:genetics*
D025521:Tumor Suppressor Proteins; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human