Proteolysis during the isoelectric focusing step of two-dimensional gel electrophoresis may be a common problem.
Finnie
Christine
C
Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500 Valby, Copenhagen, Denmark.
Svensson
Birte
B
D015180:Electrophoresis, Gel, Two-Dimensional / Q000592:standards; D010450:Endopeptidases / Q000378:metabolism*; D001467:Hordeum / Q000201:enzymology; D007525:Isoelectric Focusing / Q000592:standards*; D010446:Peptide Fragments; D011480:Protease Inhibitors; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods* / Q000592:standards; D001614:beta-Amylase / Q000302:isolation & purification / Q000378:metabolism
D010446:Peptide Fragments; D011480:Protease Inhibitors; D011506:Proteins; D001614:beta-Amylase; D010450:Endopeptidases