Caged RNase: photoactivation of the enzyme from perfect off-state by site-specific incorporation of 2-nitrobenzyl moiety.
Photo-triggered activation of semisynthetic Ribonuclease S' from a perfect off-state was successfully carried out by incorporation of photo-labile caged moiety into a proximity to the active site.
Hiraoka
Takashi
T
Institute for Fundamental Research of Organic Chemistry (IFOC), Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Fukuoka 812-8581, Japan.
Hamachi
Itaru
I
D000595:Amino Acid Sequence; D001665:Binding Sites; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009578:Nitrobenzenes / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry / Q000528:radiation effects; D010782:Photolysis; D012260:Ribonucleases / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects
D009578:Nitrobenzenes; D010446:Peptide Fragments; C023560:ribonuclease S-peptide; C036077:nitrobenzene; D012260:Ribonucleases; C022542:ribonuclease S