Meltrin beta mini, a new ADAM19 isoform lacking metalloprotease and disintegrin domains, induces morphological changes in neuronal cells.
Meltrin beta (ADAM19) is a metalloprotease-disintegrin expressed in the peripheral nervous system and other organs during embryogenesis. We report here an alternatively spliced isoform, meltrin beta mini, that lacks the prodomain, metalloprotease and disintegrin domains. A comparison of the cDNA and genomic sequences suggested the existence of a new exon. This isoform was detected in murine dorsal root ganglion and neuronal cell lines by RT-PCR. Overexpression of meltrin beta mini but not meltrin beta induced neurite outgrowth in neuronal cells. These studies suggest that the novel meltrin beta isoform has a distinct function related to neurogenesis.
Kurisaki
Tomohiro
T
Department of Growth Regulation, Institute for Frontier Medical Sciences, Kyoto University, 606-8507, Kyoto, Japan.
Wakatsuki
Shuji
S
Sehara-Fujisawa
Atsuko
A
D051722:ADAM Proteins; D017398:Alternative Splicing; D000818:Animals; D015153:Blotting, Western; D019556:COS Cells; D003545:Cysteine / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D019483:Disintegrins; D005091:Exons; D015723:Gene Library; D015151:Immunoblotting; D008565:Membrane Proteins / Q000737:chemistry*; D008666:Metalloendopeptidases; D045726:Metalloproteases; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry*; D009474:Neurons / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D019483:Disintegrins; D008565:Membrane Proteins; D009124:Muscle Proteins; D020033:Protein Isoforms; D045726:Metalloproteases; D051722:ADAM Proteins; C411902:ADAM19 protein, human; C096116:Adam19 protein, mouse; D008666:Metalloendopeptidases; D003545:Cysteine