A re-assessment of styrene-induced clastogenicity in mice in a subacute inhalation study.
To date, a number of in vivo cytogenetic assays have studied the clastogenicity (chromosome aberrations, micronuclei formation) in bone marrow of rodents exposed to styrene by various routes. The majority of all these cytogenetic experiments yielded negative findings (Scott and Preston, Mutat Res 318:175-203, 1994). Recently published data from a micronucleus test in mice exposed via inhalation for up to 21 days showed some positive response, but was not fully conclusive (Vodicka et al., Chem Biol Interact 137:213-227, 2001). Since this exposure regimen has considerable relevance for workplace exposure, the present study was performed to further elucidate these findings. NMRI mice were exposed by whole body inhalation to styrene concentrations of 750 mg/m3 and 1,500 mg/m3 for 1, 3, 7, 14 and 21 consecutive days (6 h/day). Animals were killed directly after exposure and bone marrow was sampled for analysis of micronucleus induction. Under the experimental conditions used in the present investigation, there was no evidence of clastogenicity at any concentration or exposure interval.
Engelhardt
Günter
G
Product Safety, Regulations, Toxicology and Ecology, BASF Aktiengesellschaft, 67156 Ludwigshafen, Germany. guenter.engelhardt@basf-ag.de
Gamer
Armin
A
Vodicka
Pavel
P
Bárta
Ivo
I
Hoffmann
Hans-Dieter
HD
Veenstra
Gauke
G
D000280:Administration, Inhalation; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests; D016296:Mutagenesis; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*; D016037:Single-Blind Method; D020058:Styrene / Q000008:administration & dosage / Q000633:toxicity*
D009153:Mutagens; D020058:Styrene