The conservation and regulation of rat DSP-PP gene.
Two highly expressed noncollagenous proteins associated with dentin mineralization, dentin sialoprotein (DSP) and phosphophoryn (PP), are encoded by a single DSP-PP transcript. To better understand how DSP-PP transcripts are regulated, we have determined the DSP-PP transcription start site, sequenced its 5' flanking region, and analyzed the transcriptional activity of the gene promoter out to -1615 bp. Comparison of the rat cDNA sequence with the mouse, rat and human genes clearly indicates high sequence conservation within the DSP-PP 5' flanking region, implicating the possible presence of highly conserved gene regulatory cis elements. Among a number of conserved transcription sites identified in the 5' flanking region, we demonstrate that the conserved Y box sequence (ATTGG) can specifically bind nuclear extracts from mouse MDPC23 cells. This sequence (located within the -57 bp/-52 bp 5' flanking region) therefore likely represents one DSP-PP transcriptional regulatory sequence.
Ritchie
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Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, 1011 North University, Ann Arbor, MI 48109-1078, USA. helenar@umich.edu
Li
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Hanks
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Knudtson
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Wang
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D000818:Animals; D001483:Base Sequence / Q000235:genetics; D017124:Conserved Sequence; D016326:Extracellular Matrix Proteins; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics*; D011498:Protein Precursors; D051381:Rats / Q000235:genetics*; D017207:Rats, Sprague-Dawley; D012795:Sialoglycoproteins / Q000235:genetics*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic; D000099311:Dentin Sialophosphoprotein
D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D011498:Protein Precursors; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C017630:phosphophoryn