title: Differential actions and excitotoxicity of glutamate agonists on motoneurons in adult mouse cervical spinal cord slices.
abstract: Electrical activity was recorded from motoneurons in adult mouse cervical spinal cord (C4-8) slices. Motoneurons showed almost no response to ionophoretic application of N-methyl-D-aspartic acid (NMDA) in both control and Mg(2+)-free media, but very sensitive to kainate (KA) and amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA). Bath perfusion of KA, AMPA or glucose/O(2) free Krebs-Ringer solution, but not bath perfusion of NMDA, caused membrane depolarization within 3 min and beading of the dendrite trees after more than 10 min perfusion. Our results indicate that adult motoneurons have few or no NMDA receptors.
authors.affiliation: Department of Environmental Health, School of Public Health, University at Albany, 1 University Place, Rm B242, Rensselaer, NY 12144-3456, USA.
authors.forename: N
authors.initials: N
authors.lastname: Hori
authors.forename: Y
authors.initials: Y
authors.lastname: Tan
authors.forename: M
authors.initials: M
authors.lastname: King
authors.forename: N L
authors.initials: NL
authors.lastname: Strominger
authors.forename: D O
authors.initials: DO
authors.lastname: Carpenter
mesh_terms: D000818:Animals; D002574:Cervical Vertebrae / Q000187:drug effects / Q000502:physiology; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D018698:Glutamic Acid / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009046:Motor Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists / Q000502:physiology; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
chemical_list: D018690:Excitatory Amino Acid Agonists; D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid