title: Chemotaxonomic and phylogenetic analyses of Sphingomonas strains isolated from ears of plants in the family Gramineae and a proposal of Sphingomonas roseoflava sp. nov.
abstract: Chemotaxonomic and phylogenetic characteristics of Sphingomonas strains isolated from plants of the family Gramineae were investigated. All strains contained the monosaccharide (glucuronic acid) type of glycosphingolipid (GSL-1). Most were found also to contain the oligosaccharide-type glycosphingolipids. Fatty acid and sphingosine profiles of the isolates were identical, although the ratio of the contents varies among the isolates. They all contained ubiquinone Q-10, and the G1C contents were from 66 to 68%. Phylogenetic analysis using 16S rRNA gene base sequences revealed that all the isolates were placed in the phylogenetic group of Sphingomonas paucimobilis in the alpha-4 subclass of Proteobacteria. By DNA-DNA hybridization experiments, the plant isolates were divided into five genotypic groups (groups 1 to 5). The strains of group 5 showed common physiological characteristics and formed pink-yellow colored colonies. Based on these results, Sphingomonas roseoflava sp. nov. was proposed for that homology group.
authors.affiliation: Department of Bacteriology, The Kitasato Institute, Minato-ku, Tokyo 108-8642, Japan.
authors.forename: Na Rae
authors.initials: NR
authors.lastname: Yun
authors.forename: Yong Kook
authors.initials: YK
authors.lastname: Shin
authors.forename: Se Young
authors.initials: SY
authors.lastname: Hwang
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kuraishi
authors.forename: Junta
authors.initials: J
authors.lastname: Sugiyama