title: Three different pathological lesions in the NF1 gene originating de novo in a family with neurofibromatosis type 1.
abstract: Three members of a Portuguese family, who exhibited clinical evidence of neurofibromatosis type 1 (NF1), were found to possess different heritable and pathological mutations in their NF1 genes: a 1.5-Mb deletion spanning the entire NF1 gene, a truncating CGA-->TGA transition in exon 22 (R1241X), and a frameshift mutation in exon 29 (5406insT). All three lesions occurred de novo and are likely to have been generated by different mutational mechanisms. At least two of the mutations occurred on different chromosomal backgrounds. The probability of finding three non-identical NF1 gene lesions arising de novo in a family with NF1 is very remote, too low to be readily accepted as mere coincidence. A number of possible explanations for this unique finding were therefore explored, but none were found to be wholly convincing. This report nevertheless serves as a reminder that it is unwise, even in the case of an autosomal dominant condition, to extrapolate from the detection of a single mutation in a specific individual to assuming an identical molecular genetic aetiology in other clinically affected members of the same family.
authors.affiliation: Institute of Medical Genetics, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK. upadhyaya@cardiff.ac.uk
authors.forename: Meena
authors.initials: M
authors.lastname: Upadhyaya
authors.forename: Elisa
authors.initials: E
authors.lastname: Majounie
authors.forename: Peter
authors.initials: P
authors.lastname: Thompson
authors.forename: Song
authors.initials: S
authors.lastname: Han
authors.forename: Claudia
authors.initials: C
authors.lastname: Consoli
authors.forename: Michael
authors.initials: M
authors.lastname: Krawczak
authors.forename: Isobel
authors.initials: I
authors.lastname: Cordeiro
authors.forename: David N
authors.initials: DN
authors.lastname: Cooper
mesh_terms: D000328:Adult; D002648:Child; D002886:Chromosomes, Human, Pair 17; D018389:Codon, Nonsense; D005260:Female; D016368:Frameshift Mutation; D017353:Gene Deletion; D016514:Genes, Neurofibromatosis 1; D006801:Humans; D008297:Male; D009456:Neurofibromatosis 1 / Q000235:genetics*; D010375:Pedigree
chemical_list: D018389:Codon, Nonsense