title: Distribution of HLA-DRB1 genes in patients with sporadic ankylosing spondylitis in the south of Spain.
abstract: OBJECTIVE
  To evaluate the relationship between the presence of different HLA-DRB1 genes and predisposition to develop a sporadic form of ankylosing spondylitis (AS) in a demographically well-defined population.
  
  
  METHODS
  One hundred fifteen selected patients with sporadic (non-familial) forms of AS from six different cities and 748 bone marrow donors as control group. All individuals were typed for HLA-B27 by flow cytometry with monoclonal antibodies and PCR -SSP, as well as for HLA-DRB using the Dynal ELI SSO HLA-DRB Test (Dynal AS, Oslo, Norway). The Inno-Lipa DRB Decoder (Innogenetics NV Zwijndrecht, Belgium), was used for high-resolution HLA-DRB typing.
  
  
  RESULTS
  The presence of the DRB1*01 antigen in the studied population is significantiy higher in B27 positive healthy individuals (bone marrow donors) than in B27 positive AS patients; also, DRB1*01 is higher in B27 negative AS patients than 827 negative controls. The frequency of DRB1*03 is higher in B27 negative controls than B27 negative AS patients.
  
  
  CONCLUSIONS
  The results of this study suggest that DRB1*01 antigens might be involved in the development of sporadic forms of ankylosing spondylitis in HLA-B27 negative individuals in the studied area.
authors.affiliation: Rheumatology Service, Reina Sofía University Hospital, Cordoba, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Perez-Guijo
authors.forename: E
authors.initials: E
authors.lastname: Muñoz
authors.forename: A
authors.initials: A
authors.lastname: Escudero
authors.forename: R
authors.initials: R
authors.lastname: Veroz
authors.forename: M
authors.initials: M
authors.lastname: Sánchez
authors.forename: M C
authors.initials: MC
authors.lastname: Muñoz-Villanueva
authors.forename: R
authors.initials: R
authors.lastname: González
authors.forename: J
authors.initials: J
authors.lastname: Peña
authors.forename: E
authors.initials: E
authors.lastname: Collantes-Estevez
mesh_terms: D000328:Adult; D005260:Female; D020022:Genetic Predisposition to Disease; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006239:Haplotypes; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D013030:Spain; D013167:Spondylitis, Ankylosing / Q000235:genetics*
chemical_list: D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains