Salivary gland delivery of pDNA-cationic lipoplexes elicits systemic immune responses.
OBJECTIVE
To test the ability of two cationic lipoplexes, Vaxfectin and GAP-DLRIE/DOPE, to facilitate transfection and elicit immune responses from plasmid DNAs (pDNAs) after retrograde instillation into salivary glands.


METHODS
Two pDNA expression vectors encoding either the influenza NP protein or human growth hormone (hGH) were complexed with the cationic lipid transfection reagents, GAP-DLRIE/DOPE or Vaxfectin, and delivered to the submandibular glands of rats. Samples from rats receiving the influenza NP protein pDNA and cationic lipoplexes were analyzed for anti-influenza NP-specific IgG1, IgG2a, and IgG2b in serum, and IgA in saliva, by an enzyme- linked immunosorbent assay (ELISA). Cytotoxic T-cell lymphocyte (CTL) assays were also performed. Transgene protein expression (hGH) was determined from extracts of submandibular glands of rats receiving hGH lipoplexes.


RESULTS
Serum antibodies (IgG) against the NP protein developed and were highest in all rats vaccinated with GAP-DLRIE/DOPE or Vaxfectin. The major serum IgG subclass stimulated by this route of immunization was IgG2b, followed by IgG2a. CTL assay results showed statistically significantly higher percentage killing in the Vaxfectin group than controls (P < 0.05). No rats developed IgA antibodies to NP protein in saliva. Animals receiving plasmid encoding hGH and either lipoplex expressed significantly higher amounts of hGH compared with those receiving the hGH plasmid and water. Although hGH expression was higher in the animals receiving pDNA/Vaxfectin (approximately 30-fold > pDNA/water), the difference with those receiving pDNA/GAP-DLRIE/DOPE (approximately 10-fold > pDNA/water) was not significant.


CONCLUSIONS
Retrograde instillation of pDNA complexed with Vaxfectin into the salivary glands can stimulate cytotoxic and humoral responses to the influenza NP protein antigen. Optimization of the conditions required to stimulate humoral and secretory antibody formation may facilitate use of this tissue for specific clinical applications of pDNA immunization.
Sankar
V
V
Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research (NIDCR), NIH, Bethesda, MD 20892, USA. vsankar@dir.nidcr.nih.gov
Baccaglini
L
L
Sawdey
M
M
Wheeler
C J
CJ
Pillemer
S R
SR
Baum
B J
BJ
Atkinson
J C
JC
D000704:Analysis of Variance; D000818:Animals; D000917:Antibody Formation / Q000235:genetics / Q000276:immunology*; D000956:Antigens, Viral / Q000235:genetics; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004987:Ethers / Q000008:administration & dosage; D005260:Female; D005822:Genetic Vectors / Q000235:genetics; D019382:Human Growth Hormone / Q000235:genetics; D006801:Humans; D007111:Immunity, Cellular / Q000235:genetics / Q000276:immunology*; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D009980:Influenza A virus / Q000235:genetics; D007267:Injections; D018655:Lymphocyte Count; D010714:Phosphatidylethanolamines / Q000008:administration & dosage; D010957:Plasmids / Q000008:administration & dosage*; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D013223:Statistics as Topic; D018709:Statistics, Nonparametric; D013363:Submandibular Gland / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000473:pathology; D014162:Transfection
C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D000956:Antigens, Viral; D004337:Drug Carriers; D004987:Ethers; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; C101963:N-(3-aminopropyl)-N,N-dimethyl-2,3-bis(dodecyloxy)-1-propanaminium; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; C460866:vaxfectin; D019382:Human Growth Hormone