title: First characterization of Candida albicans by random amplified polymorphic DNA method in Nicaragua and comparison of the diagnosis methods for vaginal candidiasis in Nicaraguan women.
abstract: A total of 106 women with vaginitis in Nicaragua were studied. The positive rate for the identification of Candida species was 41% (44 positive cultures out of 106 women with vaginitis). The sensitivity of microscopic examination of wet mount with the potassium hydroxide (KOH) was 61% and 70% with Gram's stain when using the culture of vaginal fluid as gold standard for diagnosis of candidiasis. Among the 44 positives cultures, isolated species of yeast from vaginal swabs were C. albicans (59%), C. tropicalis (23%), C. glabrata (14%) and C. krusei (4%). This study reports the first characterization of 26 C. albicans stocks from Nicaragua by the random amplified polymorphic DNA method. The genetic analysis in this small C. albicans population showed the existence of linkage disequilibrium, which is consistent with the hypothesis that C. albicans undergoes a clonal propagation.
authors.affiliation: Centro Nacional de Diagnostico y Referencia, Ministerio de Salud de Nicaragua, Managua, Nicararagua. madarcebello@wanadoo.fr
authors.forename: Martha
authors.initials: M
authors.lastname: Darce Bello
authors.forename: Alcides
authors.initials: A
authors.lastname: Gonzalez
authors.forename: Christian
authors.initials: C
authors.lastname: Barnabe
authors.forename: Georges
authors.initials: G
authors.lastname: Larrouy
mesh_terms: D002176:Candida albicans / Q000145:classification / Q000235:genetics*; D002181:Candidiasis, Vulvovaginal / Q000175:diagnosis* / Q000382:microbiology; D005260:Female; D006801:Humans; D009527:Nicaragua; D019105:Random Amplified Polymorphic DNA Technique; D012680:Sensitivity and Specificity