The Pseudomonas aeruginosa rhlAB operon is not expressed during the logarithmic phase of growth even in the presence of its activator RhlR and the autoinducer N-butyryl-homoserine lactone.
The Pseudomonas aeruginosa rhlAB operon encodes the enzyme rhamnosyltransferase 1, which produces the biosurfactant mono-rhamnolipid; rhlAB induction is dependent on the quorum-sensing transcription activator RhlR complexed with the autoinducer N-butyryl-homoserine lactone (C(4)-HSL). In this work we studied rhlAB induction in a P. aeruginosa and Escherichia coli background. We found that, in both bacteria, its expression is not induced during the logarithmic phase of growth even in the presence of RhlR and C(4)-HSL. Additionally, we found that rhlAB expression is partially sigma(s) dependent.
Medina
Gerardo
G
Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62251, Mexico.
Juárez
Katy
K
Soberón-Chávez
Gloria
G
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006017:Glycolipids / Q000378:metabolism; D006602:Hexosyltransferases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D009876:Operon; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D015398:Signal Transduction
D001426:Bacterial Proteins; D006017:Glycolipids; C092312:N-butyrylhomoserine lactone; C086515:RhlR protein, Pseudomonas aeruginosa; C418382:rhamnolipid; D006602:Hexosyltransferases; C088697:rhamnosyltransferase 1; D015107:4-Butyrolactone