Myocardial infarction and cardiac remodelling in mice.
We established a mouse model of cardiac dysfunction due to myocardial infarction (MI). For this we ligated the left anterior descending coronary artery in male C57BL/6J mice and assessed healing and left ventricular (LV) remodelling at 1, 2 and 4 days and 1, 2 and 4 weeks after MI. Echocardiography was performed at 1 and 2 weeks and 1, 2, 4 and 6 months after MI. We found that neutrophil infiltration of the infarct border was noticeable at 1-2 days. Marked macrophage infiltration occurred at day 4, while lymphocyte infiltration was apparent at 7-14 days. Massive proliferation of fibroblasts and collagen accumulation began by day 7-14, and scar formation was completed by day 21. LV diastolic dimension increased markedly at 2 weeks and remained at the same level thereafter. LV shortening fraction decreased significantly at 2 weeks and then slowly decreased. In non-infarcted areas of the LV, myocyte cross-sectional area and interstitial collagen fraction increased progressively, reaching a maximum at 4 months. This study provides important qualitative and quantitative information about the natural history of cardiac remodelling after MI in mice.
Yang
Fang
F
Hypertension and Vascular Research Division, Department of Internal Medicine, Henry Ford Hospital, Detroit, MI, USA.
Liu
Yun-He
YH
Yang
Xiao-Ping
XP
Xu
Jiang
J
Kapke
Alissa
A
Carretero
Oscar A
OA
D000818:Animals; D001794:Blood Pressure; D001835:Body Weight; D002455:Cell Division; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000473:pathology; D006339:Heart Rate; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009203:Myocardial Infarction / Q000401:mortality / Q000473:pathology / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000473:pathology; D009504:Neutrophils / Q000473:pathology; D009929:Organ Size; D018487:Ventricular Dysfunction, Left / Q000401:mortality / Q000473:pathology / Q000503:physiopathology; D020257:Ventricular Remodeling / Q000502:physiology*
D003094:Collagen