Protective action of nipradilol against ischemia-induced retinal damage in rats.
The purpose of this study was to investigate whether nipradilol, a beta-blocker having both vasodilating and alpha(1)-blocking activities, can protect retinal cells from the injury induced by ischemia and reperfusion. Rats were anesthetized and, after an intravitreal injection of nipradilol, the intraocular pressure was raised for 45 min to induce retinal ischemia and reperfusion. Before, and 3 and 7 days after the ischemia, electroretinograms were recorded. After the ischemia, the mean amplitude of the b-waves in rats receiving 5 microl of 1.0 x 10(-6) M nipradilol was significantly larger than of controls (injected with phosphate-buffered saline). Histologically, the reduction in the number of retinal ganglion cells (1.0 x 10(-6) M), and the thickness of the inner and outer plexiform layers and the inner nuclear layer (1.0 x 10(-6), 10(-7) and 10(-8) M) was suppressed by nipradilol. These results indicate that nipradilol protected the retina against retinal ischemia and reperfusion and should be considered for therapeutic use in cases of transient retinal ischemia.
Taniai
Mariko
M
Department of Ophthalmology and Visual Science, Graduate School of Medicine, Chiba University, Chiba, Japan. taniai@ophthalm.m.chiba-u.ac.jp
Sato
Eiju
E
Mizota
Atsushi
A
Adachi-Usami
Emiko
E
D000818:Animals; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004596:Electroretinography / Q000187:drug effects / Q000379:methods; D007511:Ischemia / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D011412:Propanolamines / Q000627:therapeutic use*; D020011:Protective Agents / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury; D012164:Retinal Diseases / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D014665:Vasodilator Agents / Q000627:therapeutic use*
D011412:Propanolamines; D020011:Protective Agents; D014665:Vasodilator Agents; C036089:nipradilol