title: Simultaneous horizontal gene transfer of a gene coding for ribosomal protein l27 and operational genes in Arthrobacter sp.
abstract: Phylogenetic analysis of bacterial L27 ribosomal proteins showed that, against taxonomy, the L27 protein from the Actinobacteria Arthrobacter sp. clusters with protein sequences from the Bacillus group. The L27 gene clusters in the Arthrobacter sp. genome with six genes responsible for creatinine and sarcosine degradation. Phylogenetic analyses of orthologue proteins encoded by three of these genes also showed a phylogenetic relationship with Bacillus species. Comparisons between the synonymous codon usage of the Arthrobacter sp. genes and those from complete genomes showed that Arthrobacter genes encoding the L27 ribosomal protein and the proteins responsible for the degradation of creatinine and sarcosine have a codon usage that is more similar to that of Bacillus species than that of Arthrobacter. We suggest that the Arthrobacter sp. genes encoding the L27 ribosomal protein and the proteins responsible for the degradation of creatinine and sarcosine were acquired simultaneously through horizontal gene transfer from an unknown Bacillus species.
authors.affiliation: Department of Biochemistry and Biotechnology, Rovira i Virgili University, Pl Imperial Tàrraco 1, E-43005 Tarragona, Catalonia, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Garcia-Vallvé
authors.forename: Francesc X
authors.initials: FX
authors.lastname: Simó
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Montero
authors.forename: Lluís
authors.initials: L
authors.lastname: Arola
authors.forename: Antoni
authors.initials: A
authors.lastname: Romeu
mesh_terms: D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000235:genetics*; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D012269:Ribosomal Proteins; C034661:ribosomal proteins L27