Effects of homocysteine on the cellular responses of murine endothelial cells cultured in vitro.
Homocysteine, derived from the metabolism of methionine, is claimed as a proatherogenic factor that leads to vascular dysfunction. To gain better insight into the molecular mechanisms involved, homocysteine was tested on a model of murine endothelial cells cultured in vitro, using a prototype DNA chip. The DNA chip was designed to follow the expression at the mRNA level of some major proinflammatory genes; TNF-alpha was used as a positive control.
Tacheny
A
A
Department of Biology, Laboratory of Cellular Biochemistry and Biology, University of Namur (FUNDP), Namur, Belgium.
dos Santos
S
S
Huet
A-C
AC
van Steenbrugge
M
M
Michiels
C
C
Holvoet
P
P
Raes
M
M
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D006710:Homocysteine / Q000633:toxicity*; D007249:Inflammation / Q000235:genetics; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D014409:Tumor Necrosis Factor-alpha; D006710:Homocysteine