Evidence for packaging of rep-cap sequences into adeno-associated virus (AAV) type 2 capsids in the absence of inverted terminal repeats: a model for generation of rep-positive AAV particles.
We previously reported that a 350-bp region of the adeno-associated virus (AAV) type 2 rep gene contains a cis-acting element responsible for the Rep-dependent replication of a transiently transfected rep-cap plasmid. In this study, we further report that replicated rep-cap sequences can be packaged into AAV capsids in the absence of the inverted terminal repeats.
Nony
Pascale
P
INSERM ERM 0105, CHU Hotel-Dieu, 44035 Nantes, France.
Chadeuf
Gilliane
G
Tessier
Jacques
J
Moullier
Philippe
P
Salvetti
Anna
A
D002213:Capsid / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000235:genetics*; D000229:Dependovirus / Q000502:physiology*; D010957:Plasmids; D020079:Terminal Repeat Sequences; D014764:Viral Proteins / Q000235:genetics*; D014771:Virion / Q000502:physiology*; D019065:Virus Assembly
D004268:DNA-Binding Proteins; D014764:Viral Proteins; C068734:rep proteins, Adeno-associated virus 2