Analysis of microsatellite polymorphism around the HLA-B locus in Iranian patients with Behçet's disease.
We have previously suggested that in a Japanese population the susceptible locus for Behçet's disease (BD) is HLA-B51 itself. To confirm this finding in another population, we performed HLA class I typing using the PCR-SSP method and analyzed eight polymorphic markers distributed within 1100 kb around the HLA-B gene using automated sequencer and subsequent automated fragment detection by fluorescent-based technology with the DNA samples of 84 Iranian patients with BD and 87 healthy ethnically matched controls. As a result, three microsatellite alleles (MICA-A6, MIB-348, C1-4-1-217) and HLA-B51 were found to be strongly associated with BD. Of these alleles HLA-B51 is the most strongly associated allele. There were no alleles that were increased in allele frequency at any microsatellite loci centromeric of MICA or telomeric of HLA-B51. Therefore, HLA-B51 was confirmed to be by far the most strongly associated gene with BD in an Iranian population.
Mizuki
N
N
Department of Ophthalmology, Yokohama City University School of Medicine, Kanagawa, Japan.
Yabuki
K
K
Ota
M
M
Katsuyama
Y
Y
Ando
H
H
Nomura
E
E
Funakoshi
K
K
Davatchi
F
F
Chams
H
H
Nikbin
B
B
Ghaderi
A A
AA
Ohno
S
S
Inoko
H
H
D001528:Behcet Syndrome / Q000235:genetics* / Q000276:immunology; D002874:Chromosome Mapping; D015235:HLA-B Antigens / Q000235:genetics*; D059949:HLA-B51 Antigen; D006801:Humans; D007492:Iran; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic
D015235:HLA-B Antigens; D059949:HLA-B51 Antigen