L-MBP is expressed in epithelial cells of mouse small intestine.
The mannan-binding proteins (L-MBP and S-MBP, also denoted MBL-C and MBL-A), mainly produced in liver and existing in liver and serum, play important roles in the innate immunity against a variety of pathogens. Total RNA from mouse tissues were screened for MBP mRNA by RT-PCR. In addition to liver, S-MBP mRNA was detected in lung, kidney, and testis, and L-MBP mRNA was detected in kidney, thymus, and small intestine. Quantitative RT-PCR revealed that the small intestine is a predominant site of extrahepatic expression of L-MBP. Western blotting with polyclonal Abs against rat L-MBP demonstrated this protein in Triton X-100 extracts of the small intestine obtained from mice that had undergone systemic perfusion. Immunohistochemical staining with an mAb against mouse L-MBP and in situ hybridization revealed that L-MBP is selectively expressed in some villous epithelial cells of the small intestine. These findings suggest that L-MBP plays a role in mucosal innate immunity.
Uemura
Kazuhide
K
Department of Biological Chemistry, Core Research for Science and Technology Project, Japan Science and Technology Corp., Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.
Saka
Masako
M
Nakagawa
Tomoaki
T
Kawasaki
Nobuko
N
Thiel
Steffen
S
Jensenius
Jens Christian
JC
Kawasaki
Toshisuke
T
D000818:Animals; D022781:Hepatocytes / Q000737:chemistry / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000737:chemistry / Q000378:metabolism*; D007421:Intestine, Small / Q000737:chemistry / Q000378:metabolism*; D007668:Kidney / Q000737:chemistry / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008168:Lung / Q000737:chemistry / Q000378:metabolism; D008297:Male; D037601:Mannose-Binding Lectin / Q000031:analogs & derivatives* / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000737:chemistry / Q000378:metabolism
D037601:Mannose-Binding Lectin; D020033:Protein Isoforms; D012333:RNA, Messenger; C069858:mannose binding protein A