Erythrocyte response to near-infrared radiation.
The effects of NIR (near-infrared radiation 700-2,000 nm) on bovine erythrocytes in plasma was studied as a continuation of earlier studies. Cell shape was observed and the changes of ratio of hemolysis and electrokinetic potential measured as a function of irradiation time. After 10 min of irradiation, the shape of erythrocyte cells was mainly echinocytic. When these cells were incubated at 311 K for 24 h they regained their initial shape, but fresh erythrocytes that were irradiated for 30 min and aged in vitro did not. These phenomena are due to: (1) the absorption of NIR excitation by hemoglobin; the primary photochemical process being the photo-dissociation of oxyhemoglobin to deoxyhemoglobin. Resulting shape and ratio of hemolysis, structural changes and oxidative stress follow higher deoxyhemoglobin concentration. (2) The absorption of the NIR excitation by proteins, water and lipids. After NIR absorption the membrane surface dehydrates, leading to enhanced protonation and dissociation of hydrogen-bonded complexes. This in turn leads to a change in electrokinetic potential.
Komorowska
Małgorzata
M
Institute of Physics, Wrocław University of Technology, Wyb. Wyspiańskiego 27, 50-370 Wrocław, Poland. kom@rainbow.if.pwr.wroc.pl
Cuissot
Arnaud
A
Czarnołeski
Adam
A
Białas
Wieslaw
W
D000818:Animals; D002417:Cattle; D048429:Cell Size / Q000528:radiation effects; D004586:Electrophoresis; D004912:Erythrocytes / Q000166:cytology / Q000502:physiology / Q000528:radiation effects*; D006461:Hemolysis / Q000528:radiation effects*; D066298:In Vitro Techniques; D007259:Infrared Rays; D007700:Kinetics; D011199:Potentiometry; D013997:Time Factors