[Ultrastructural observation of normal spermatozoa incubated with ROS in vitro].
OBJECTIVE
To observe ultrastructural changes of spermatozoa after incubate with reactive oxygen species (ROS).


METHODS
Spermatozoa of normal physiological functions selected from semen samples by Percoll gradient centrifugation technique were regarded as normal sperm models in present study. Ultrastructural changes of spermatozoa observed by transmission electron microscope after model spermatozoa were incubated with ROS generated by hypoxanthine and xanthine oxidase under aerobic environment.


RESULTS
After model spermatozoa were incubated with ROS, impairment of various extent in membrane and acrosome of spermatozoa and abnormality in mitochondria of spermatozoa were found.


CONCLUSIONS
Excessive ROS may cause ultrastructural change in membrane, acrosome and mitochondria of spermatozoa and impair function of spermatozoa.
Shang
Xue-Jun
XJ
Laboratory of Reproduction & Genetics, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China.
Huang
Yu-Feng
YF
Xiong
Cheng-Liang
CL
Yin
Hong-Lin
HL
Wang
Yong-Mei
YM
D000328:Adult; D006801:Humans; D008297:Male; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D013094:Spermatozoa / Q000187:drug effects* / Q000648:ultrastructure
D017382:Reactive Oxygen Species