title: Effect of triclosan dentifrices on mouth volatile sulphur compounds and dental plaque trypsin-like activity during experimental gingivitis development.
abstract: BACKGROUND
  The objective of this study was to evaluate the effect of three commercial anti-plaque dentifrices containing 0.3% triclosan + 2% pvm/ma (Colgate Total), 0.3% triclosan + 0.75% Zn (Signal Global) and 0.3% triclosan + 5% PPi (Crest Complete) in comparison with an experimental dentifrice (0.3% triclosan + 2% pvm/ma + 0.75% Zn + 4% PPi) and a control dentifrice without anti-plaque agents on trypsin-like activity in dental plaque (detected by the hydrolysis of [Na-Benzoyl-DL-Anginine p-Nitroanilide (BAPNA)] and volatile sulphur compounds (VSCs) in mouth air during experimental gingivitis development.
  
  
  METHODS
  A 5-step double blind, crossover experimental gingivitis study was conducted on 19 volunteers during a 21-day period. The volunteers refrained from brushing an experimental quadrant of teeth. The dentifrices were applied to those teeth via toothshield three times per day; simultaneously they brushed the other teeth with the same dentifrice. After each period, VSCs in mouth air and BAPNA hydrolysis by dental plaque accumulated in the experimental quadrant were determined.
  
  
  RESULTS
  There was an increase (p < 0.05) in VSCs in mouth air when experimental gingivitis was induced in only one quadrant of teeth. None of the dentifrices was able to avoid the increase of VSCs during the experimental gingivitis development. The majority of the antiplaque dentifrices evaluated reduced the increase of VSC formation in comparison with the control (p < 0.05). There was no relationship between the ability of the dentifrices in reducing VSC formation and the inhibition of trypsin-like activity in dental plaque.
  
  
  CONCLUSIONS
  Anti-plaque dentifrices reduce the increase of VSCs that occurs during the development of experimental gingivitis.
authors.affiliation: Department of Periodontics, Faculty of Dentistry, Bahia Science Foundation, Salvador, Bahia, Brazil.
authors.forename: G R
authors.initials: GR
authors.lastname: Nogueira-Filho
authors.forename: P M
authors.initials: PM
authors.lastname: Duarte
authors.forename: S
authors.initials: S
authors.lastname: Toledo
authors.forename: C P M
authors.initials: CP
authors.lastname: Tabchoury
authors.forename: J A
authors.initials: JA
authors.lastname: Cury
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D001586:Benzoylarginine Nitroanilide; D001944:Breath Tests; D018592:Cross-Over Studies; D003773:Dental Plaque / Q000188:drug therapy* / Q000201:enzymology; D003802:Dentifrices / Q000627:therapeutic use*; D004311:Double-Blind Method; D005260:Female; D005891:Gingivitis / Q000378:metabolism*; D006209:Halitosis / Q000517:prevention & control*; D006801:Humans; D008297:Male; D018709:Statistics, Nonparametric; D013457:Sulfur Compounds / Q000378:metabolism*; D014260:Triclosan / Q000008:administration & dosage / Q000627:therapeutic use*; D014357:Trypsin / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D003802:Dentifrices; D013457:Sulfur Compounds; D014260:Triclosan; D001586:Benzoylarginine Nitroanilide; D014357:Trypsin