Carbohydrase activities of the rolC-gene transformed and non-transformed ginseng cultures.
The levels of activity of beta-D-glucosidase, alpha-D-mannosidase, alpha- and beta-D-galactosidase, 1,3-, 1,6-, 1,4-beta-D-glucanases, 1,4-alpha-D-glucanase, fucoidanhydrolase and agarase were measured in extracts of non-transgenic and transgenic Panax ginseng cultures transformed with the rolC gene. Significantly increased levels of activity of beta- and alpha-D-galactosidases and 1,3-beta-D-glucanase were detected in rolC-gene transformed cells, compared to the control non-transformed cells, while levels of activity of other enzymes were unchanged. These, as well as the gel-permeation experiments, revealed that transformation of ginseng cells by the rolC gene could significantly affect activity of some carbohydrases and production of their molecular forms.
Bulgakov
V P
VP
Far East Branch of Russian Academy of Sciences, Laboratory of Biotechnology, Institute of Biology and Soil Science, Prop Stoletija, 159, Vladivostok 690022, Russia. biotech@eastnet.febras.ru
Kusaykin
M
M
Tchernoded
G K
GK
Zvyagintseva
T N
TN
Zhuravlev
Yu N
YN
D002478:Cells, Cultured; D004790:Enzyme Induction; D005798:Genes, Bacterial / Q000235:genetics*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D005894:Panax / Q000201:enzymology* / Q000235:genetics*; D030821:Plants, Genetically Modified; D012231:Rhizobium / Q000235:genetics; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism*
D006026:Glycoside Hydrolases; C019822:carbohydrase; C071257:cytokinin-beta-glucosidase; D001617:beta-Glucosidase