Protective effect of Nardostachys jatamansi in rat cerebral ischemia.
The protective effect of Nardostachys jatamansi (NJ) on neurobehavioral activities, thiobarbituric acid reactive substance (TBARS), reduced glutathione (GSH), thiol group, catalase and sodium-potassium ATPase activities was studied in middle cerebral artery (MCA) occlusion model of acute cerebral ischemia in rats. The right MCA of male Wistar rats was occluded for 2 h using intraluminal 4-0 monofilament and reperfusion was allowed for 22 h. MCA occlusion caused significant depletion in the contents of glutathione and thiol group and a significant elevation in the level of TBARS. The activities of Na(+)K(+) ATPase and catalase were decreased significantly by MCA occlusion. The neurobehavioral activities (spontaneous motor activity and motor coordination) were also decreased significantly in MCA occlusion group. All the alternations induced by ischemia were significantly attenuated by 15 days pretreatment of NJ (250 mg/kg po) and correlated well with histopathology by decreasing the neuronal cell death following MCA occlusion and reperfusion. The study provides first evidence of effectiveness of NJ in focal ischemia most probably by virtue of its antioxidant property.
Salim
Sofiyan
S
Neurotoxicology Laboratory, Department of Medical Elementology and Toxicology, Jamia Hamdard University, Hamdard Nagar, New Delhi 110062, India. fislam2001@yahoo.co.in
Ahmad
Muzamil
M
Zafar
Khan Shoeb
KS
Ahmad
Abdullah Shafique
AS
Islam
Fakhrul
F
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001921:Brain / Q000201:enzymology; D001923:Brain Chemistry / Q000502:physiology; D002545:Brain Ischemia / Q000188:drug therapy* / Q000473:pathology*; D002374:Catalase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D032404:Nardostachys / Q000737:chemistry*; D018696:Neuroprotective Agents; D010936:Plant Extracts / Q000627:therapeutic use; D004856:Postural Balance / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D018696:Neuroprotective Agents; D010936:Plant Extracts; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione