title: Survey of restriction-modification systems and transformation in Mannheimia haemolytica and Pasteurella trehalosi.
abstract: A significant obstacle to molecular studies of Mannheimia (Pasteurella) haemolytica, has been its resistance to genetic transformation. The lack of competence of many M. haemolytica strains has been attributed to the presence of restriction modification systems. In this study, representative strains of 12 M. haemolytica serotypes and four Pasteurella trehalosi serotypes were successfully transformed by electroporation using a recombinant vector derived from the native M. haemolytica A1 serotype plasmid pNSF2176. Transformation was achieved despite PCR-based evidence for the presence of genes encoding a type I restriction enzyme, phaI, and a type II restriction enzyme hsdM, in each of the M. haemolytica strains.
authors.affiliation: Moredun Research Institute, International Research Centre, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, Edinburgh, Scotland EH26 0PZ, UK. a.hill@hw.ac.uk
authors.forename: A E
authors.initials: AE
authors.lastname: Hill
authors.forename: F A
authors.initials: FA
authors.lastname: Lainson
mesh_terms: D015280:DNA Restriction-Modification Enzymes; D018274:Electroporation; D005822:Genetic Vectors; D016978:Mannheimia haemolytica / Q000737:chemistry / Q000235:genetics*; D010325:Pasteurella / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014169:Transformation, Bacterial / Q000235:genetics*
chemical_list: D015280:DNA Restriction-Modification Enzymes