[Effects of cyclosporin A on expression of FasL and Fas in the contralateral testis after the unilateral testis injured in KM mouse].
OBJECTIVE
To investigate the effects of Cyclosporin A (CsA) on spermatogenesis and expression of FasL and Fas in the contralateral testis after the unilateral testis was injured.


METHODS
60 mice were randomly divided equally into groups A (control), B (the unilateral testis was injured by glacial acetic acid), C (excision of ipsilateral testis at 6 hours after the unilateral testis was injured by glacial acetic acid) and D (CsA within 6 hours after the unilateral testis was injured by glacial acetic acid). Sperm density and sperm motility were evaluated after 4 weeks. Expression of FasL and Fas was performed by immunohistochemistry (SP method). The positive cells with SP staining in seminiferous tubules were calculated.


RESULTS
Sperm density and sperm motility in group D were significantly increased compared with group B(P < 0.05). Expression of FasL and Fas in group D decreased significantly compared with group B (24.3 +/- 7.0 vs 37.8 +/- 5.8 and 17.8 +/- 4.3 vs 32.4 +/- 3.6, P < 0.05).


CONCLUSIONS
CsA decreased expression of Fas and FasL and maintained spermatogenesis in the contralateral testis after the unilateral testis was injured by glacial acetic acid.
Shu
Yong
Y
Department of Urology, Second College of Clinical Medicine, Chongqing University of Medical Sciences, Chongqing 400010, China.
Yang
Guang-Yong
GY
Zhang
Wei-Li
WL
Hu
Zi-Li
ZL
D000818:Animals; D016572:Cyclosporine / Q000494:pharmacology*; D004195:Disease Models, Animal; D053222:Fas Ligand Protein; D015870:Gene Expression / Q000187:drug effects*; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D051379:Mice; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D019014:fas Receptor / Q000096:biosynthesis*
D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008562:Membrane Glycoproteins; D019014:fas Receptor; D016572:Cyclosporine