title: Directional restriction site-free insertion of PCR products into vectors.
authors.affiliation: F. Hoffmann La-Roche, Basel, Switzerland.
authors.forename: Guo Jun
authors.initials: GJ
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases / Q000235:genetics; D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific; D005822:Genetic Vectors; D007202:Indicators and Reagents; D016254:Mutagenesis, Insertional / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping
chemical_list: D017931:DNA Primers; D007202:Indicators and Reagents; D002265:Carboxylic Ester Hydrolases; C019674:aminoacyl-tRNA hydrolase; C020102:endodeoxyribonuclease DpnI; D015252:Deoxyribonucleases, Type II Site-Specific