1,25-Dihydroxyvitamin D(3) increases striatal GDNF mRNA and protein expression in adult rats.
Glial cell line-derived neurotrophic factor (GDNF) has been postulated as a possible candidate for therapeutic treatment in Parkinson's disease (PD). Recent in vitro data suggest that 1,25-dihydroxyvitamin D3 [1,25(OH)(2)D(3)] treatment may enhance GDNF mRNA expression. In the present study, using semiquantitative RT-PCR and Western blot, we have shown that 1,25(OH)(2)D(3) administration intraperitoneally, significantly increases GDNF mRNA and protein levels in the striatum of adult rats.
Sanchez
Begoña
B
Department of Physiology, School of Medicine, University of Santiago de Compostela, Spain.
Lopez-Martin
Elena
E
Segura
Carmen
C
Labandeira-Garcia
Jose L
JL
Perez-Fernandez
Roman
R
D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002120:Calcium Channel Agonists / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects* / Q000502:physiology; D051100:Glial Cell Line-Derived Neurotrophic Factor; D006801:Humans; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D010300:Parkinson Disease / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic
D002120:Calcium Channel Agonists; C495039:GDNF protein, human; C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D002117:Calcitriol