[Preliminary study on hens immunized with recombinant human testis prostaglandin D synthase DNA].
OBJECTIVE
In order to identify whether the hens immunized with recombinant human testis prostaglandin D synthase (rhtL-PGDS) DNA can produce anti-L-PGDS antibody.


METHODS
The serum were got from the hens immunized with recombinant plasmid pGEX-2T/htL-PGDS DNA (100 micrograms) every 2 weeks for 2 times. The exist of anti-L-PGDS antibody and its titer were tested with agarose dual immunodiffusion and ELISA with rhtL-PGDS as antigen.


RESULTS
The serum anti-L-PGDS antibody in hen immunized with pGEX-2T/htL-PGDS DNA were confirmed and its titer tested by ELISA was 1:2,048.


CONCLUSIONS
It is feasible to produce anti-L-PGDS antibody by immunizing hens with recombinant pGEX-2T/htL-PGES DNA.
Lu
Jin-Chun
JC
Clinical Laboratory, Nanjing Hospital of the Chinese Armed Police Force, Nanjing, Jiangsu 210024, China.
Huang
Yu-Feng
YF
Zhang
Xi-Ran
XR
D000818:Animals; D000906:Antibodies / Q000032:analysis; D002645:Chickens; D004274:DNA, Recombinant / Q000008:administration & dosage / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D007114:Immunization; D019746:Intramolecular Oxidoreductases / Q000235:genetics* / Q000276:immunology; D054834:Lipocalins; D008297:Male; D023421:Models, Animal; D010957:Plasmids / Q000235:genetics; D013737:Testis / Q000201:enzymology; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology
D000906:Antibodies; D004274:DNA, Recombinant; D054834:Lipocalins; D019444:Vaccines, DNA; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase