title: In vitro toxicity of stimulant soft drinks.
abstract: Some drinks containing natural products with stimulant properties are highly consumed among young adults. The market for these drinks has increased in past years around the globe, and, although they might be harmless, overdoses or combination of these with other drinks could be harmful to the health of some consumers in certain circumstances. Samples were obtained at food shops and different popular brands were chosen. Cytotoxicity tests used were neutral red uptake, total protein content, and tetrazolium assay on Chinese hamster ovary cells. Results revealed that tested samples were not cytotoxic; however, studies have demonstrated the toxicity of high concentrations of some of these products. For this reason, the authors considered it to be of critical importance to carryout an in vitro toxicity screening of stimulant soft drinks that are highly consumed.
authors.affiliation: Department of Biotecnología, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain. asantamaria@isciii.es
authors.forename: A
authors.initials: A
authors.lastname: Santa-María
authors.forename: M M
authors.initials: MM
authors.lastname: Díaz
authors.forename: A
authors.initials: A
authors.lastname: López
authors.forename: M T
authors.initials: MT
authors.lastname: de Miguel
authors.forename: M J
authors.initials: MJ
authors.lastname: Fernández
authors.forename: A I
authors.initials: AI
authors.lastname: Ortiz
mesh_terms: D000818:Animals; D001628:Beverages / Q000633:toxicity*; D001681:Biological Assay; D016466:CHO Cells; D000697:Central Nervous System Stimulants / Q000633:toxicity*; D004396:Coloring Agents / Q000493:pharmacokinetics; D006224:Cricetinae; D009499:Neutral Red / Q000493:pharmacokinetics; D011506:Proteins / Q000032:analysis; D013778:Tetrazolium Salts / Q000378:metabolism
chemical_list: D000697:Central Nervous System Stimulants; D004396:Coloring Agents; D011506:Proteins; D013778:Tetrazolium Salts; D009499:Neutral Red