Chlamydia pneumoniae infection alters the junctional complex proteins of human brain microvascular endothelial cells.
Chlamydia pneumoniae has been identified and associated with multiple sclerosis (MS) and Alzheimer's disease (AD) pathogenesis, although the relationship of this organism in these diseases remains controversial. We have hypothesized that one potential avenue of infection is through the junctional complexes between the blood-brain barrier (BBB) endothelia. C. pneumoniae is characteristically a respiratory pathogen, but has been implicated in atherosclerosis, coronary artery disease, and neuroinflammatory conditions. C. pneumoniae infection may lead to endothelial damage, junctional alterations, and BBB breakdown. Therefore, in this study, C. pneumoniae infection of human brain microvascular endothelial cells (HBMECs) resulted in increased expression of the zonula adherens proteins beta-catenin, N-cadherin, and VE-cadherin, and decreased expression of the tight junctional protein occludin, as determined by immunocytochemistry and Western blot analyses. These events may underlie a mechanism for the regulation of paracellular permeability while maintaining barrier integrity during C. pneumoniae infection associated with neuropathologies such as MS and AD.
MacIntyre
Angela
A
Department of Biomedical Sciences, Philadelphia College of Osteopathic Medicine, 4170 City Ave, Philadelphia, PA 19131, USA.
Hammond
Christine J
CJ
Little
C Scott
CS
Appelt
Denah M
DM
Balin
Brian J
BJ
D015703:Antigens, CD; D001921:Brain / Q000098:blood supply* / Q000166:cytology; D015820:Cadherins / Q000378:metabolism; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D023521:Chlamydophila Infections / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000378:metabolism / Q000472:pathogenicity*; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000382:microbiology* / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology; D062793:Occludin; D019108:Tight Junctions / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology; D051176:beta Catenin; D000099152:Cadherin 5
D015703:Antigens, CD; D015820:Cadherins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D062793:Occludin; D015534:Trans-Activators; D051176:beta Catenin; D000099152:Cadherin 5; C495270:CTNNB1 protein, human; C568985:OCLN protein, human