title: Extracellular polysaccharides of Erwinia futululu, a bacterium associated with a fungal canker disease of Eucalyptus spp.
abstract: Extracellular polysaccharides (EPSs) produced by an Erwinia spp. associated with a fungal canker disease of Eucalyptus were fractionated into two polysaccharides, one that was identified with that produced by Erwinia stewartii. The other has a similar structure, but with one terminal Glc residue replaced by pyruvic acid to give 4,6-O-[(R)-1-carboxyethylidene)-Galp. Their structures were determined using a combination of chemical and physical techniques including methylation analysis, periodate oxidation, low-pressure gel filtration and anion-exchange chromatographies, high-pH anion-exchange chromatography, mass spectrometry and 1D and 2D 1H NMR spectroscopy. The new polysaccharides, identified as EPS Futululu FF-1 and FF-2, have the following structures:The molecular weights of the polysaccharides range from 1.3-2.1x10(6) and their hydrodynamic properties are those of polydisperse, polyanionic biopolymers with pseudoplastic, non-thixotropic flow characteristics in aqueous solutions.
authors.affiliation: Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA.
authors.forename: Byung Yun
authors.initials: BY
authors.lastname: Yang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Ding
authors.forename: Rex
authors.initials: R
authors.lastname: Montgomery
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005591:Chemical Fractionation; D002965:Classification; D004885:Erwinia / Q000737:chemistry* / Q000145:classification; D005052:Eucalyptus / Q000382:microbiology; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification*; D021521:Ribotyping
chemical_list: D011135:Polysaccharides, Bacterial