[Up-expression of IL-6 and down-expression of TNFalpha may be involved in the regulation of apoptosis induced by antisense bcl-2 oligodeoxynucleotides].
OBJECTIVE
To investigate the regulation mechanism of apoptosis induced by the antisense bcl-2 treatment.


METHODS
DNA content analysis and terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) were adopted to detect apoptosis. Semi-quantitative reverse transcription-PCR was performed to detect the mRNA expression of bcl-2 c-myc survivin bax s100A(2) TNFalpha TGFbeta(1) and IL-6 in the small-cell lung cancer cell line NCI-H446 treated with antisense bcl-2 oligodeoxynucliotide.


RESULTS
bcl-2 AS-PS-ODN treatment could induce apoptosis, accompanied with 72.71% up-regulation of IL-6 and 65.90% down-regulation of TNFalpha, whereas little or no effect was seen on c-myc survivin bax s100A(2) and TGFbeta(1).


CONCLUSIONS
IL-6 and TNFalpha may be involved in the regulation of apoptosis induced by antisense bcl-2 treatment.
Chen
Xinji
X
Union Hospital of Fujian Medical University, Fujian Institute of Hematology, Fuzhou, Fujian, 350001 P. R. China.
Hu
Jianda
J
Chen
Zhizhe
Z
Lu
Lianhuang
L
D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D002630:Chemotactic Factors / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D020287:In Situ Nick-End Labeling; D051034:Inhibitor of Apoptosis Proteins; D015850:Interleukin-6 / Q000235:genetics*; D008869:Microtubule-Associated Proteins / Q000235:genetics; D009363:Neoplasm Proteins; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D009418:S100 Proteins / Q000235:genetics; D000077022:Survivin; D016212:Transforming Growth Factor beta / Q000235:genetics; D053773:Transforming Growth Factor beta1; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; C107489:BIRC5 protein, human; D002630:Chemotactic Factors; D051034:Inhibitor of Apoptosis Proteins; D015850:Interleukin-6; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D016376:Oligonucleotides, Antisense; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D009418:S100 Proteins; C098997:S100A2 protein, human; D000077022:Survivin; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D051028:bcl-2-Associated X Protein