title: Micronucleus induction in mussels exposed to okadaic acid.
abstract: Some toxins present in the marine environment are capable of inducing mutagenicity and/or carcinogenicity. Among these toxins, okadaic acid (OA) is gaining considerable interest since it induces DNA based modifications at low concentrations and accumulates in filter-feeding marine animals, including those used for human consumption. This study aims to evaluate the genotoxicity of OA in the haemocytes of the mussel Perna perna, using the micronucleus assay. Fifty-four mussels were separated into three groups of 18 animals. One group received 0.3 microg of OA diluted in 10 microl of ethanol and ultrapure water while the other groups were considered as controls and were exposed to a solvent plus seawater mixture. A significantly higher frequency of micronuclei was observed in haemocytes from the OA-exposed group. There were no statistical differences between the two control groups.
authors.affiliation: Department of Environmental Engineering, Toxicological Laboratory of the Federal University of Santa Catarina, Brazil.
authors.forename: C R
authors.initials: CR
authors.lastname: Carvalho Pinto-Silva
authors.forename: J F
authors.initials: JF
authors.lastname: Ferreira
authors.forename: R H R
authors.initials: RH
authors.lastname: Costa
authors.forename: P
authors.initials: P
authors.lastname: Belli Filho
authors.forename: E E
authors.initials: EE
authors.lastname: Creppy
authors.forename: W G
authors.initials: WG
authors.lastname: Matias
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000187:drug effects* / Q000235:genetics*; D004784:Environmental Monitoring / Q000379:methods; D006434:Hemocytes / Q000187:drug effects*; D008387:Marine Toxins / Q000633:toxicity*; D015162:Micronucleus Tests; D019319:Okadaic Acid / Q000633:toxicity*; D013997:Time Factors
chemical_list: D008387:Marine Toxins; D019319:Okadaic Acid