title: [Dopamine receptors and calcium channels regulating striatal inhibitory synaptic transmission].
abstract: A whole-cell patch-clamp study was performed to investigate the modulatory role of dopamine (DA), its ionic mechanisms and their developmental changes in the GABAergic synaptic transmission onto cholinergic interneurones in the rat striatal slices. Inhibitory postsynaptic currents (IPSCs) were evoked by focal stimulation. Bath application of DA inhibited the IPSCs in a concentration-dependent manner with an IC50 value of 10 microM. Pharmacological studies with DA receptor agonists and antagonists suggest the involvement of D2-like receptors. DA reduced the frequency of miniature inhibitory postsynaptic currents without affecting their amplitude distribution. Analyses using selective blockers for N-, or P/Q type Ca2+ channels could estimate the contribution of each Ca2+ channel subtype to the GABAergic transmission. DA had no longer affected the IPSCs after the effect of an N-type channel blocker, omega-conotoxin (omega-CgTX) had reached its steady state. The inhibitory effects of omega-CgTX and DA or a D2-like receptor agonist decreased in parallel during postnatal 12-60 days. DA's action was occluded by omega-CgTX throughout these developmental stages. These results suggest that activation of presynaptic D2-like receptors selectively blocks N-type Ca2+ channels, thereby inhibiting GABA release, and that contribution of N-type channels and D2-like receptor-mediated presynaptic inhibition decrease in parallel with development.
authors.affiliation: Division of Cerebral Structure, National Institute for Physiological Sciences, Okazaki 444-8585, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Momiyama
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020864:Calcium Channels, N-Type / Q000502:physiology*; D002799:Cholinergic Fibers / Q000502:physiology; D003342:Corpus Striatum / Q000502:physiology*; D003864:Depression, Chemical; D004298:Dopamine / Q000494:pharmacology / Q000502:physiology; D065127:Dopamine D2 Receptor Antagonists; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D018408:Patch-Clamp Techniques; D051381:Rats; D017448:Receptors, Dopamine D2 / Q000819:agonists / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism / Q000502:physiology; D020960:omega-Conotoxins / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D020864:Calcium Channels, N-Type; D065127:Dopamine D2 Receptor Antagonists; D017448:Receptors, Dopamine D2; D020960:omega-Conotoxins; D005680:gamma-Aminobutyric Acid; D004298:Dopamine