Precision and accuracy of a HPLC method for measurement of fecal porphyrin concentrations.
We studied precision and accuracy of a HPLC method for determination of porphyrins in feces. A commercial standard solution appeared to contain less coproporphyrin (15%) than stated by the manufacturer. The between-batch coefficients of variation were often below 15% and were higher than the within-batch coefficients. The precision of porphyrin measurements was not influenced by the type of porphyria. Recoveries of added coproporphyrin and protoporphyrin were 90% and 108%; coefficients of variation were 6% and 19%, respectively.
Zuijderhoudt
Floris M J
FM
Department of Clinical Chemistry, Deventer Ziekenhuis, Deventer, The Netherlands. Zuijderf@DZ.nl
Kamphuis
Johannes S
JS
Kluitenberg
Willem E
WE
Dorresteijn-de Bok
Jenneke
J
D002851:Chromatography, High Pressure Liquid; D003306:Coproporphyrins / Q000032:analysis; D005243:Feces / Q000737:chemistry*; D006801:Humans; D011164:Porphyrias / Q000175:diagnosis*; D011166:Porphyrins / Q000032:analysis*; D011524:Protoporphyrins / Q000032:analysis; D012680:Sensitivity and Specificity
D003306:Coproporphyrins; D011166:Porphyrins; D011524:Protoporphyrins; C026971:coproporphyrin III; C031467:coproporphyrin I