Bone formation in the context of growth retardation induced by hIGFBP-1 overexpression in transgenic mice.
In humans, intrauterine growth retardation (hIUGR) is correlated with an overexpression of insulin-like growth factor binding protein 1 (IGFBP-1). The affected children also present a delay in bone mineralization. In this study, transgenic 12-day-old mutant mice overexpressing human IGFBP-1 hepatospecifically showed a severe growth retardation. Alcian blue and alizarin red S staining of the skeleton revealed mineralization defects at the posterior level of the skull (delayed suture closure) and in appendicular and axial skeleton. Furthermore, microradiographic analysis showed a reduced bone density in the same areas. Thus, overexpressing of hIGFBP-1 demonstrates early postnatal life growth retardation and a delay in mineralization in transgenic mutant mice. These data show the involvement of the IGF/IGFBP system and more particularly IGFBP-1 in the biomineralization process.
Ben Lagha
N
N
Laboratoire de Biologie-Orofaciale et Pathologie, INSERM EMI U-0110, Université Paris 7, IFR 58, Institut Biomédical des Cordeliers, Esc. E, 2è ét., 15-21 rue de l'Ecole de Médecine, 75270 Paris, France. Pierrette.Menuelle@bhdc.jussieu.fr
Menuelle
P
P
Seurin
D
D
Binoux
M
M
Lebouc
Y
Y
Berdal
A
A
D000818:Animals; D000831:Animals, Newborn; D015519:Bone Density; D006130:Growth Disorders / Q000139:chemically induced* / Q000000981:diagnostic imaging / Q000503:physiopathology*; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics; D010012:Osteogenesis; D011859:Radiography
D018970:Insulin-Like Growth Factor Binding Protein 1