title: Nested MWC model describes hydrolysis of GroEL without assuming negative cooperativity in binding.
abstract: Folding assistance and ATPase activity of GroEL are based on the existence of different conformations. In order to characterise these conformations, published data on steady state ATPase activity in the absence of GroES were reanalysed simultaneously in terms of the Nested MWC model. This model is a hierarchical extension of the symmetry-model of Monod et al. [J. Mol. Biol. 12 (1965) 88]. An unique set of GroEL specific parameters was obtained. This set was supported by comparison of predictions arising from this set of values with experimental data for hydrolysis of ATP in the presence of ADP and ATPgammaS, binding of ATPgammaS and ADP to GroEL in the absence of ATP, and binding of ATP as monitored by fluorescence labelling. Thus, for the first time, multiple data sets for the interaction of nucleotides with GroEL are described quantitatively by an allosteric model. A noteworthy feature of our model is that no negative cooperativity in ATP binding occurs in accordance to experimental observations. Furthermore, the model also includes the existence of a conformation with very high ATPase activity. Such a conformation might be of importance at a certain stage in the folding cycle.
authors.affiliation: Institute for Molecular Biophysics, University of Mainz, Germany. nadja@biophysik.biologie.uni-mainz.de
authors.forename: Nadja
authors.initials: N
authors.lastname: Hellmann
authors.forename: Heinz
authors.initials: H
authors.lastname: Decker
mesh_terms: D000244:Adenosine Diphosphate; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives*; D002138:Calibration; D018834:Chaperonin 60 / Q000378:metabolism*; D003198:Computer Simulation; D006868:Hydrolysis; D008958:Models, Molecular; D011485:Protein Binding
chemical_list: D018834:Chaperonin 60; C022571:adenosine 5'-O-(3-thiotriphosphate); D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases