title: Sequence analysis of a Hoxa4-Hoxa5 intergenic region including shared regulatory elements.
abstract: The dynamic patterns of Hox gene expression are generated by a combination of regulatory elements differentially controlling Hox gene transcription in a spatio-temporal and tissue-specific fashion. Regulatory elements can either be exclusive for a particular gene, shared by neighboring Hox genes, or be competed for by different promoters. Our interest in Hoxa5 gene function and regulation have led us to localize Hoxa5 cis-acting regulatory elements in the intergenic region between the Hoxa4 and Hoxa5 genes and involved in Hoxa5 organ expression. Mapping of the region and sequence analysis revealed some discrepancies with a previously published sequence. Here, we report an additional DNA fragment of 282 base pairs that possesses high homology with the human HOX sequence, and contains potential binding sites for trans-acting factors.
authors.affiliation: Centre de recherche en cancérologie de l'Université Laval, Centre Hospitalier Universitaire de Québec, L'Hôtel-Dieu de Québec, 9, rue McMahon, Québec, Que., Canada, G1R 2J6.
authors.forename: Julie
authors.initials: J
authors.lastname: Moreau
authors.forename: Lucie
authors.initials: L
authors.lastname: Jeannotte
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D021901:DNA, Intergenic / Q000235:genetics*; D004268:DNA-Binding Proteins; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000032:analysis / Q000235:genetics*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D008969:Molecular Sequence Data; D010750:Phosphoproteins; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D014157:Transcription Factors
chemical_list: D021901:DNA, Intergenic; D004268:DNA-Binding Proteins; C489213:HOXA5 protein, human; D018398:Homeodomain Proteins; C484111:Hoxa4 protein, mouse; C059637:Hoxa5 protein, mouse; D010750:Phosphoproteins; D014157:Transcription Factors; C073547:HOXA4 protein, human