title: Molecular fingerprinting of fusidic acid- and rifampicin-resistant strains of methicillin-resistant Staphylococcus aureus (MRSA) from Malaysian hospitals.
abstract: The emergence and spread of multiresistant methicillin-resistant Staphylococcus aureus (MRSA) strains, especially those resistant to fusidic acid and rifampicin, in Malaysian hospitals is of concern. In this study DNA fingerprinting by PFGE was performed on fusidic acid- and rifampicin-resistant isolates from Malaysian hospitals to determine the genetic relatedness of these isolates and their relationship with the endemic MRSA strains. In all, 32 of 640 MRSA isolates from 9 Malaysian hospitals were resistant to fusidic acid and rifampicin. Seven PFGE types (A, ZC, ZI, ZJ, ZK, ZL and ZM) were observed. The commonest type was type ZC, seen in 72% of isolates followed by type A, seen in 13%. Each of the other types (ZI, ZJ, ZK, ZL and ZM) was observed in a single isolate. Each type, even the commonest, was found in only one hospital. This suggests that the resistant strains had arisen from individual MRSA strains in each hospital and not as a result of the transmission of a common clone.
authors.forename: A
authors.initials: A
authors.lastname: Norazah
authors.forename: V K E
authors.initials: VKE
authors.lastname: Lim
authors.forename: Y T
authors.initials: YT
authors.lastname: Koh
authors.forename: M Y
authors.initials: MY
authors.lastname: Rohani
authors.affiliation: Bacteriology Unit, Infectious Diseases Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, *Hospital Umum Sarawak, Jalan Tun Abang Haji Openg, 93590 Kuching, Sarawak, †Hospital Queen Elizabeth, Jalan Mat Salleh, 88590 Kota Kinabalu, Sabah, ‡Hospital Alor Setar, Jalan Sultan Badlishah, 05590 Alor Setar, Kedah and §Faculty of Allied Health Sciences, National University of Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: H
authors.initials: H
authors.lastname: Zuridah
authors.affiliation: Bacteriology Unit, Infectious Diseases Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, *Hospital Umum Sarawak, Jalan Tun Abang Haji Openg, 93590 Kuching, Sarawak, †Hospital Queen Elizabeth, Jalan Mat Salleh, 88590 Kota Kinabalu, Sabah, ‡Hospital Alor Setar, Jalan Sultan Badlishah, 05590 Alor Setar, Kedah and §Faculty of Allied Health Sciences, National University of Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: K
authors.initials: K
authors.lastname: Spencer
authors.affiliation: Bacteriology Unit, Infectious Diseases Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, *Hospital Umum Sarawak, Jalan Tun Abang Haji Openg, 93590 Kuching, Sarawak, †Hospital Queen Elizabeth, Jalan Mat Salleh, 88590 Kota Kinabalu, Sabah, ‡Hospital Alor Setar, Jalan Sultan Badlishah, 05590 Alor Setar, Kedah and §Faculty of Allied Health Sciences, National University of Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: P P
authors.initials: PP
authors.lastname: Ng
authors.affiliation: Bacteriology Unit, Infectious Diseases Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, *Hospital Umum Sarawak, Jalan Tun Abang Haji Openg, 93590 Kuching, Sarawak, †Hospital Queen Elizabeth, Jalan Mat Salleh, 88590 Kota Kinabalu, Sabah, ‡Hospital Alor Setar, Jalan Sultan Badlishah, 05590 Alor Setar, Kedah and §Faculty of Allied Health Sciences, National University of Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: A G M
authors.initials: AGM
authors.lastname: Kamel
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016172:DNA Fingerprinting / Q000379:methods*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005672:Fusidic Acid / Q000494:pharmacology*; D006801:Humans; D008296:Malaysia / Q000453:epidemiology; D016106:Methicillin Resistance / Q000235:genetics; D008826:Microbial Sensitivity Tests; D012293:Rifampin / Q000494:pharmacology*; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D005672:Fusidic Acid; D012293:Rifampin