title: Loss of G protein gamma 7 alters behavior and reduces striatal alpha(olf) level and cAMP production.
abstract: The G protein beta gamma-dimer is required for receptor interaction and effector regulation. However, previous approaches have not identified the physiologic roles of individual subtypes in these processes. We used a gene knockout approach to demonstrate a unique role for the G protein gamma(7)-subunit in mice. Notably, deletion of Gng7 caused behavioral changes that were associated with reductions in the alpha(olf)-subunit content and adenylyl cyclase activity of the striatum. These data demonstrate that an individual gamma-subunit contributes to the specificity of a given signaling pathway and controls the formation or stability of a particular G protein heterotrimer.
authors.affiliation: Weis Center for Research, Geisinger Clinic, Danville, Pennsylvania 17822, USA.
authors.forename: William F
authors.initials: WF
authors.lastname: Schwindinger
authors.forename: Kelly S
authors.initials: KS
authors.lastname: Betz
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Giger
authors.forename: Angela
authors.initials: A
authors.lastname: Sabol
authors.forename: Sarah K
authors.initials: SK
authors.lastname: Bronson
authors.forename: Janet D
authors.initials: JD
authors.lastname: Robishaw
mesh_terms: D000818:Animals; D001483:Base Sequence; D003342:Corpus Striatum / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D017931:DNA Primers; D019281:Dimerization; D005260:Female; D044385:GTP-Binding Protein alpha Subunits; D020962:Heterotrimeric GTP-Binding Proteins / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D044385:GTP-Binding Protein alpha Subunits; C084618:olfactory G protein subunit alpha olf; D000242:Cyclic AMP; D020962:Heterotrimeric GTP-Binding Proteins