Site-directed photochemical disruption of the actin cytoskeleton by actin-binding Rose Bengal-conjugates.
The in situ light-induced, non-enzymatic digestion of cytoskeletal actin by a xanthene dye conjugated to heavy meromyosin, anti-actin antibodies and/or anti-myosin antibodies is reported. The dye Rose Bengal was conjugated to either anti-actin antibodies, anti-myosin antibodies or heavy meromyosin. Under our experimental conditions, visible light induced the non-enzymatic breakdown of cytoskeletal actin when mammalian tissue culture cells were probed either with Rose Bengal-conjugated anti-actin and/or anti-myosin antibodies. Similar results were obtained when tissue culture cells were probed with Rose Bengal-conjugated heavy meromyosin before irradiation with visible light. The in situ photochemical reaction depended on the presence of actin-binding Rose Bengal-conjugates.
Conlon
Kimberly A
KA
Department of Pharmacological Sciences, School of Medicine, University Hospital and Medical Center, State University of New York at Stony Brook, Stony Brook, NY 11794-8651, USA. conlon@pharm.sunysb.edu
Rosenquist
Thomas
T
Berrios
Miguel
M
D000199:Actins / Q000378:metabolism / Q000528:radiation effects*; D000818:Animals; D001665:Binding Sites; D002645:Chickens; D003599:Cytoskeleton / Q000528:radiation effects*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D015879:Myosin Subfragments / Q000378:metabolism / Q000528:radiation effects; D009218:Myosins / Q000378:metabolism / Q000528:radiation effects; D010777:Photochemistry; D011817:Rabbits; D012395:Rose Bengal / Q000493:pharmacokinetics*
D000199:Actins; D005456:Fluorescent Dyes; D015879:Myosin Subfragments; D012395:Rose Bengal; D009218:Myosins