title: Adipose depletion and apoptosis induced by trans-10, cis-12 conjugated linoleic Acid in mice.
abstract: OBJECTIVE
  To compare the effectiveness of a conjugated linoleic acid (CLA) isomer mixture (mCLA) with each main isomer [trans-10,cis-12 CLA (CLA10,12) and cis-9,trans-11 CLA (CLA9,11)] in causing body lipid loss and adipose tissue apoptosis.
  
  
  METHODS
  Mice selected over 16 generations for high (MH) or low (ML) energy expenditure and a control group (MC) were fed diets containing either soy oil or soy oil plus mCLA, CLA10,12, or CLA9,11 for 5 days in one study and 14 days in a second study.
  
  
  RESULTS
  Mice fed mCLA or CLA10,12 had less body lipid (p < 0.05), smaller retroperitoneal fat pads (p < 0.05), and ate less (p < 0.01) than mice fed no CLA or CLA9,11 for 5 days. Mice consuming 1% mCLA or 0.5% CLA10,12 gained less weight (p < 0.01) and had less body lipid (p < 0.05) and smaller epididymal (p < 0.05) and retroperitoneal fat pads (p < 0.01) than mice consuming either control or 0.5% CLA9,11-containing diets for 14 days. Only mCLA and CLA10,12 increased apoptosis in retroperitoneal fat pads (p < 0.01). The effects of mCLA and CLA10,12 were independent of genetic line except for the effect on adipocyte apoptosis. Mice of the MH line were slightly less sensitive than MC or ML mice to CLA-induced adipose tissue apoptosis.
  
  
  CONCLUSIONS
  CLA10,12, but not CLA9,11, can induce both body fat loss and adipose apoptosis. Although mice of a genotype with less body fat and greater metabolic rate and feed intake appear less sensitive, these CLA effects are robust for mice of varying metabolic background.
authors.affiliation: Department of Animal Science, University of Nebraska, Lincoln, USA.
authors.forename: Kimberly M
authors.initials: KM
authors.lastname: Hargrave
authors.forename: ChangLong
authors.initials: C
authors.lastname: Li
authors.forename: Brett J
authors.initials: BJ
authors.lastname: Meyer
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Kachman
authors.forename: Diane L
authors.initials: DL
authors.lastname: Hartzell
authors.forename: Mary Anne
authors.initials: MA
authors.lastname: Della-Fera
authors.forename: Jess L
authors.initials: JL
authors.lastname: Miner
authors.forename: Clifton A
authors.initials: CA
authors.lastname: Baile
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000033:anatomy & histology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001823:Body Composition / Q000187:drug effects*; D001835:Body Weight; D004032:Diet; D004435:Eating; D004822:Epididymis / Q000033:anatomy & histology; D005260:Female; D019787:Linoleic Acid / Q000008:administration & dosage*; D008055:Lipids / Q000032:analysis; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size; D012187:Retroperitoneal Space
chemical_list: D008055:Lipids; D019787:Linoleic Acid