[Relationship between recurrence and metastasis of gastric cancer and expression of EGFR, IL-6R, PCNA, and DI].
OBJECTIVE
The primary reasons for the failure were recurrence and metastasis in the treatment of gastric cancer. This study was designed to investigate the distributive regularity of EGFR, IL-6R, PCNA, DI and the diploid in the gastric cancer patients with recurrence and metastasis, to provide reliable basis to judge whether it has metastasis before operation and recurrence post operation.


METHODS
Immunohistochemistry SP method and image analyzer (Fegulgen staining) were used to test the EGFR, IL-6R, PCNA, DI and the diploid in 199 patients with gastric cancer, 20 cases with normal gastric mucous membrane, and 20 cases of atypical hyperplasia.


RESULTS
The level of expression of EGFR was higher in the patients with metastasis and residual gastric cancer(61.11%, 66%, 66.67%) than in the patients with other cancers, normal gastric mucous membrane, atypical hyperplasia(47.83%, 0%, 35%) (P < 0.01); In the distribution form of DNA diploid, the rate of non-diploid cells was remarkably higher in the patients with metastasis and residual gastric cancer(56.9 +/- 13.3% vs 12.8 +/- 6.3%, 0%, 12.2), and so is the DNA index (2.91 +/- 0.33 vs 2.17 +/- 0.29), but the rate of 2C cells is lower(43.8 +/- 12.9 vs 10.2 +/- 7.5, P < 0.01).


CONCLUSIONS
EGFR, DI, and the diploid are valuable targets for judging metastasis and recurrence of gastric cancer before and after operation.
Wang
Zhi-hui
ZH
Department of Pathology, Linyi Tumor Hospital, Linyi 276000, P. R. China.
Wang
Chun-ying
CY
Liu
Xiao-jiang
XJ
Wang
Lu-guang
LG
Li
Ming
M
Chen
Chang-zhao
CZ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004273:DNA, Neoplasm / Q000032:analysis*; D066246:ErbB Receptors / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis*; D019947:Receptors, Interleukin-6 / Q000096:biosynthesis*; D012008:Recurrence; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*
D004273:DNA, Neoplasm; D018809:Proliferating Cell Nuclear Antigen; D019947:Receptors, Interleukin-6; D066246:ErbB Receptors