title: A DNase I hypersensitive site flanks an origin of DNA replication and amplification in Sciara.
abstract: In chromosomes of metazoa, the assembly of the genome into chromatin makes an important but poorly understood contribution to determining where DNA replication will initiate. We addressed this issue by studying the developmental progression of the location of the DNA replication origin (ORI) and alterations in chromatin structure in one of the best-mapped ORIs in metazoa, that found in DNA puff II/9A of the fly Sciara coprophila. We found that DNA synthesis for both normal chromosomal endoduplication and DNA amplification initiates within the same 5.5 kb EcoRI fragment. We showed that irrespective of the mode of ORI function--replication or amplification--chromatin over the 1 kb major ORI is never remodeled into a conventional DNase I hypersensitive site (DH site). Instead, we found that the major site of alterations to chromatin structure at this locus is a large (approximately 400 bp) DH site located 600 bp away from the major ORI, at a position where the frequency of replication initiation events falls dramatically. We describe a tight positive correlation between ORI activity, strength of this DH site, and the intranuclear titer of protein factor(s) that bind the DH site in a sequence-specific manner. We propose that the Sciara replicator in locus II/9A is composed of sequences that reside within the ORI per se as well as sequences encompassed by the DH site.
authors.affiliation: Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Division of Biology and Medicine, Providence, RI 02912, USA.
authors.forename: Fyodor D
authors.initials: FD
authors.lastname: Urnov
authors.forename: Chun
authors.initials: C
authors.lastname: Liang
authors.forename: Hannah G
authors.initials: HG
authors.lastname: Blitzblau
authors.forename: Heidi S
authors.initials: HS
authors.lastname: Smith
authors.forename: Susan A
authors.initials: SA
authors.lastname: Gerbi
mesh_terms: D000818:Animals; D001483:Base Sequence; D004261:DNA Replication; D003850:Deoxyribonuclease I / Q000378:metabolism*; D004175:Diptera / Q000235:genetics*; D007814:Larva / Q000235:genetics*; D008969:Molecular Sequence Data; D018741:Replication Origin / Q000235:genetics*
chemical_list: D003850:Deoxyribonuclease I