CCl4-induced acute liver injury in mice is inhibited by hepatocyte growth factor overexpression but stimulated by NK2 overexpression.
Hepatocyte growth factor (HGF) inhibits acute liver injury. NK2 acts as an antagonist to HGF in vitro, but its in vivo function has reached no consensus conclusions. We have investigated in vivo effects of HGF and NK2 on CCl4-induced acute liver injury. Elevation of the serum alanine aminotransferase level and extension of centrilobular necrosis were inhibited in HGF transgenic mice but were promoted in NK2 transgenic mice. Hepatocyte proliferation after liver injury was not inhibited in NK2 transgenic mice. Thus, this study indicates that HGF inhibits liver injury, and NK2 antagonizes HGF on liver injury, however, NK2 may not antagonize HGF on hepatocyte proliferation.
Otsuka
Toshiyuki
T
First Department of Internal Medicine, Gunma University School of Medicine, 3-39-15 Showa, Gunma 371-8511, Maebashi, Japan. otsukato@showa.gunma-u.ac.jp
Takagi
Hitoshi
H
Horiguchi
Norio
N
Toyoda
Mitsuo
M
Sato
Ken
K
Takayama
Hisashi
H
Mori
Masatomo
M
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D015152:Blotting, Northern; D002251:Carbon Tetrachloride / Q000494:pharmacology*; D002455:Cell Division; D018076:DNA, Complementary / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000473:pathology*; D051379:Mice; D008822:Mice, Transgenic; D009336:Necrosis; D017434:Protein Structure, Tertiary; D012313:RNA / Q000378:metabolism; D013997:Time Factors; D019076:Transgenes
D018076:DNA, Complementary; D012313:RNA; D017228:Hepatocyte Growth Factor; D002251:Carbon Tetrachloride; D000410:Alanine Transaminase