Encapsulated Bifidobacterium bifidum potentiates intestinal IgA production.
We asked whether Bifidobacterium bifidum regulates the synthesis of IgA by mucosal lymphoid cells. B. bifidum alone, but not Clostridium perfringens, significantly induced total IgA and IgM synthesis by both mesenteric lymph nodes (MLN) and Peyer's patch (PP) cells. We, further, investigated the mucosal antibody production following peroral administration of B. bifidum to mice. Ingested B. bifidum significantly increased the number of Ig (IgM, IgG, and IgA) secreting cells in the culture of both MLN and spleen cells. Nonetheless, B. bifidum itself does not induce the own specific antibody responses, implying that B. bifidum does not provoke unnecessary immune reaction. Subsequently, it was found that encapsulation of B. bifidum further augments the total IgA production in the culture of both MLN and spleen cells. Finally, we found that the immuno-stimulating activity of B. bifidum is due to its cellular components but not due to any actively secreting component(s) from bacteria.
Park
Ji Hee
JH
Department of Microbiology, Kangwon National University, 200-701, Chunchon, Republic of Korea.
Um
Ju In
JI
Lee
Beom Jin
BJ
Goh
Juhn Su
JS
Park
Seung Yong
SY
Kim
Wan Sup
WS
Kim
Pyeung Hyeun
PH
D000276:Adjuvants, Immunologic; D000464:Alginates; D000818:Animals; D001644:Bifidobacterium; D002452:Cell Count; D002478:Cells, Cultured; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007114:Immunization; D007070:Immunoglobulin A / Q000032:analysis / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008643:Mesentery / Q000276:immunology; D051379:Mice; D010581:Peyer's Patches / Q000276:immunology; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000276:Adjuvants, Immunologic; D000464:Alginates; D006603:Hexuronic Acids; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D020723:Glucuronic Acid