title: Age-related increase of protein glycation in peripheral blood lymphocytes is restricted to preferential target proteins.
abstract: Advanced glycation end products (AGE) have been analyzed in aging human peripheral blood lymphocytes since protein glycation and glycoxidation are believed to contribute to the intracellular age-related accumulation of damaged proteins, a process that has been associated with the cellular functional deficits that occur with age. The appearance of AGE in cell lysates was monitored with an enzyme-linked immunosorbent assay using an anti-AGE antibody raised against glycated RNAse. When lymphocyte cytosolic extracts from old donors (86-91 years old) were compared with those from young donors (20-25 years old), a small but significant 40% increase of protein glycation was observed. In both age groups, further analysis of the pattern of glycated proteins by two-dimensional gel electrophoresis followed by western blotting with the same anti-AGE antibody, showed that the protein silver stain and the immunoblot patterns were not superimposable indicating that glycoxidative modifications are targeting only a restricted set of proteins. Among these preferential protein targets, seven of them exhibited a significant age-related increased immunoreactivity with the anti-AGE antibody suggesting that the corresponding modified proteins might serve as biomarkers of aging lymphocytes.
authors.affiliation: Laboratoire de Biologie et Biochimie Cellulaire du Vieillissement, Université Denis Diderot-Paris 7, CC 7128, 2 place Jussieu, 75251 Paris Cedex 05, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Poggioli
authors.forename: Hilaire
authors.initials: H
authors.lastname: Bakala
authors.forename: Bertrand
authors.initials: B
authors.lastname: Friguet
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D016922:Cellular Senescence / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D017127:Glycation End Products, Advanced / Q000032:analysis*; D006031:Glycosylation; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*
chemical_list: D017127:Glycation End Products, Advanced; D011506:Proteins