Effects of flavonoids on cisplatin-induced apoptosis of HL-60 and L1210 leukemia cells.
Effects of three flavonoids, quercetin (QU), galangin (GA), and chrysin (ChR) on cisplatin (cis-Pt)-induced apoptosis of human promyelocytic leukemia HL-60 cells and murine leukemia L1210 cells were investigated. The quantitative analysis of apoptotic DNA fragmentation was used to show that preincubation of cells with flavonoids can influence cis-Pt-induced apoptosis in different way. ChR had no effect, QU enhanced, and GA reduced apoptotic DNA fragmentation. It is also shown that combined treatment with QU and cis-Pt showed synergistic effect, however, GA combined with cis-Pt exhibited antagonism on cytotoxicity in L1210 murine leukemia cells. We assume that tested flavonoids affect the important biological activities connected with cancer chemotherapy and chemoprevention as they differently modulated the sensitivity of cells to cis-Pt treatment. QU is presented as pro-apoptotic agent and GA as agent with anti-apoptotic potential.
Cipák
Lubos
L
Cancer Research Institute, Vlárska 7, 833 91, Bratislava, Slovak Republic.
Rauko
Peter
P
Miadoková
Eva
E
Cipáková
Ingrid
I
Novotný
Ladislav
L
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects; D004273:DNA, Neoplasm / Q000187:drug effects; D004347:Drug Interactions; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D018922:HL-60 Cells / Q000187:drug effects*; D006801:Humans; D007939:Leukemia L1210 / Q000473:pathology*; D051379:Mice; D015394:Molecular Structure; D011794:Quercetin / Q000737:chemistry / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004273:DNA, Neoplasm; D005419:Flavonoids; C037032:galangin; C043561:chrysin; D011794:Quercetin; D002945:Cisplatin