[Application of Agilent 2100 Bioanalyzer in the study of differential gene expression].
OBJECTIVE
To study the application of Agilent 2100 Bioanalyzer in the study of gene differential expression.


METHODS
The total RNAs were extracted and purified from Saccharomyces cerevisiae to synthesize double-stranded cDNAs by reverse transcription. Restriction display-PCR was employed to obtain the cDNA fragments, which were examined by Agilent 2100 Bioanalyzer and agarose gel electrophoresis.


RESULTS
The analysis showed that Agilent 2100 Bioanalyzer was more sensitive and faster to isolate and display the differentially expressed genes, providing at the same time accurate quantitative information for each fragment in the DNA sample.


CONCLUSIONS
Agilent 2100 Bioanalyzer can be instrumental for the study of differential gene expression.
Liu
Cui-Hua
CH
Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.
Ma
Wen-Li
WL
Shi
Rong
R
Ouyang
Qian
Q
Zheng
Wen-Ling
WL
D018076:DNA, Complementary / Q000032:analysis; D004587:Electrophoresis, Agar Gel / Q000379:methods; D015870:Gene Expression; D020869:Gene Expression Profiling; D006360:Heat-Shock Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012680:Sensitivity and Specificity
D018076:DNA, Complementary; D006360:Heat-Shock Proteins