Involvement of bcl-family expression in the spatial memory impairment induced by repeated ischemia.
In the present study, we examined the effects of repeated ischemia (10 min x 2, 1 hr interval) on spatial memory in rats in an 8-arm radial maze test compared with single ischemia (10 min x 1). Repeated ischemia produced more severe impairment of spatial memory and stronger TUNEL-positive immunoreactivity in the hippocampal CA1 region than single ischemia at 7 days after reperfusion. Moreover, repeated ischemia altered bcl-family expression, which is related to apoptosis, while this was not affected by single ischemia. These results suggest that spatial memory impairment at 7 days after repeated ischemia may be related to apoptosis in hippocampal CA1 cells.
Irie
Keiichi
K
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, 814-0180, Fukuoka, Japan.
Mishima
Kenichi
K
Ishibashi
Daisuke
D
Egashira
Nobuaki
N
Iwasaki
Katsunori
K
Fujiwara
Michihiro
M
D000109:Acetylcholine / Q000502:physiology; D000818:Animals; D016923:Cell Death; D019940:Genes, bcl-1 / Q000502:physiology*; D006624:Hippocampus / Q000473:pathology; D020925:Hypoxia-Ischemia, Brain / Q000235:genetics* / Q000473:pathology / Q000523:psychology*; D020287:In Situ Nick-End Labeling; D008297:Male; D018782:Maze Learning / Q000502:physiology; D008569:Memory Disorders / Q000235:genetics* / Q000473:pathology / Q000523:psychology*; D018377:Neurotransmitter Agents / Q000502:physiology; D009638:Norepinephrine / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000235:genetics* / Q000473:pathology / Q000523:psychology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013028:Space Perception / Q000502:physiology*
D018377:Neurotransmitter Agents; D000109:Acetylcholine; D009638:Norepinephrine