title: Diayangambin exerts immunosuppressive and anti-inflammatory effects in vitro and in vivo.
abstract: In this study, the furofuran lignan (+)-diayangambin [tetrahydro-1,4-bis(3,4,5-trimethoxyphenyl)-(1 R)-1alpha,3abeta,4alpha,6abeta-1 H,3 H-furo [3,4- c]furan] was evaluated in vitro and in vivo for its immunomodulatory and anti-inflammatory efficacy. Human mononuclear cell proliferation was inhibited by diayangambin with an IC 50 value of 1.5 (0.5 - 2.8) microM. In addition, the compound reduced for 40.8 % prostaglandin E 2 generation in stimulated RAW 264.7 macrophage cell line at 10 microM. In vivo, a clear reduction of ear swelling was observed when diayangambin (40 mg/kg) was administered orally to 2,4-dinitrofluorobenzene-treated mice. The inhibition of swelling was associated with a reduction of leukocyte infiltration determined as myeloperoxidase activity. In the carrageenan mouse paw edema model, diayangambin significantly suppressed inflamed paw volume and prostaglandin E 2 levels. Our findings indicate the potential interest of diayangambin in the treatment of immune and inflammatory responses.
authors.affiliation: Centro de Investigaciones Farmacognósticas de la Flora Panameña (CIFLORPAN), Facultad de Farmacia, Universidad de Panamá, Panamá.
authors.forename: Elvira J
authors.initials: EJ
authors.lastname: De León
authors.forename: Dionisio A
authors.initials: DA
authors.lastname: Olmedo
authors.forename: Pablo N
authors.initials: PN
authors.lastname: Solís
authors.forename: Mahabir P
authors.initials: MP
authors.lastname: Gupta
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Terencio
mesh_terms: D000818:Animals; D000840:Anisoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology* / Q000627:therapeutic use; D001379:Azathioprine / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D004487:Edema / Q000188:drug therapy; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology* / Q000627:therapeutic use; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D008213:Lymphocyte Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D008517:Phytotherapy; D031701:Piper; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000840:Anisoles; D000894:Anti-Inflammatory Agents, Non-Steroidal; D005663:Furans; D007166:Immunosuppressive Agents; D010936:Plant Extracts; C472151:diayangambin; D003907:Dexamethasone; D015232:Dinoprostone; D001379:Azathioprine