title: Effects of intensity and duration of exercise on total leukocyte count in normal subjects.
abstract: BACKGROUND
  Leucocytes the mobile units of the protective system of the body, may circulate freely in the blood, adhere to the vascular endothelium in sites where blood flow is relatively slow and then once again re-enter the circulation in a process of continuous exchange. This process of continuous exchange of leucocytes is influenced by proper stimulation such as exercise. This study was done to observe the effects of intensity and duration of exercise on total leucocyte count.
  
  
  METHODS
  Two groups of normal healthy male adult subjects were exercised on treadmill. Group-I performed exercise by running for a duration of 2-3 minutes, at 7% grade (4 degree inclination) and at constant speed ranging from 7.5 to 9 Km/hour while group-II walked on treadmill for a duration of 7-10 minutes at zero grade and at constant speed ranging from 5 to 6 Km/hour. Blood sample was drawn before exercise, immediately after exercise and after 30 minutes of rest after the exercise. Total leukocyte count was then done by improved Neubauer haemocytometer.
  
  
  RESULTS
  The mean of pre-exercise total leucocyte count of Group-I subjects (n = 15) was 9750 +/- 384.47/microliter, while immediately after exercise the mean total leucocyte count increased to 17,856.66 +/- 1213.24/microliter and after 30 minutes rest, it came down to 10,396.67 +/- 648.35/microliter. Whereas, the mean of total leucocyte count of group-II subjects (n = 15) was 10,266.66 +/- 307.60/microliter pre-exercise, 16,336.66 +/- 866.30/microliter immediately post-exercise and 1162.33 +/- 718.85/microliter at 30 minutes post-exercise. The total leucocyte count increased significantly immediately after exercise and it significantly decreased after 30 minutes of rest but was still higher than the pre-exercise count in both the groups
  
  
  CONCLUSIONS
  It is clearly reflected by this study that the magnitude of exercise-induced leucocytosis is higher in exercise of more intensity and less duration.
authors.affiliation: Department of Physiology, Gomal Medical College, DI Khan.
authors.forename: Shaukat
authors.initials: S
authors.lastname: Ali
authors.forename: Farman
authors.initials: F
authors.lastname: Ullah
authors.forename: Habib
authors.initials: H
authors.lastname: Ullah
mesh_terms: D000328:Adult; D005080:Exercise Test; D006801:Humans; D007958:Leukocyte Count; D007964:Leukocytosis / Q000175:diagnosis; D008297:Male; D010807:Physical Endurance / Q000502:physiology*; D011336:Probability; D011446:Prospective Studies; D012016:Reference Values; D012420:Running / Q000502:physiology*; D012680:Sensitivity and Specificity; D013997:Time Factors