title: (+)-Catechin inhibits platelet hyperactivity induced by an acute iron load in vivo.
abstract: Reactive oxygen species and platelets are thought to be involved in the pathogenesis of cardiovascular disease. Epidemiological data have indicated that high consumption of fruits and vegetables is associated with a lower incidence of vascular events. Polyphenols were proposed to provide such a protection. In the present study performed in rats, we investigated the influence of (+)-catechin (Cat), a polyphenol identified in tea, cocoa, and red wine, on an acute iron load-induced model of platelet hyperactivity. We found that platelet function was significantly enhanced in iron-loaded rats. These changes were associated with impairment of the antioxidative defense including ex vivo free radical-induced hemolysis. Pretreatment with Cat (10 mg/kg, ip, 4 d) normalized biomarkers of antioxidative status and platelet hyperactivity. The benefits of Cat treatment were only observed in iron-loaded animals and not in control animals. In light of the known antioxidant properties of Cat (or its metabolites), we suggest that oxidative injury-induced modification of platelet calcium homeostasis may have explained the iron load-induced platelet hyperactivity. The protective effect of Cat appears to work probably through normalization of the antioxidative status.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM), Dijon, France. blache@dijon.inra.fr
authors.forename: Denis
authors.initials: D
authors.lastname: Blache
authors.forename: Philippe
authors.initials: P
authors.lastname: Durand
authors.forename: Michel
authors.initials: M
authors.lastname: Prost
authors.forename: Nadine
authors.initials: N
authors.lastname: Loreau
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism*; D002392:Catechin / Q000494:pharmacology*; D007501:Iron / Q000097:blood / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002392:Catechin; D007501:Iron