title: Cell membrane modification for rapid display of proteins as a novel means of immunomodulation: FasL-decorated cells prevent islet graft rejection.
abstract: Long-term display of exogenous proteins on the cell surface may have important research and therapeutic implications. We report a novel method for the cell-surface display of proteins that involves generation of a chimeric protein with core streptavidin, biotinylation of cells, and "decoration" with the protein. A chimeric protein with the extracellular portions of FasL (SA-FasL) was efficiently displayed on the cell surface within 2 hr without detectable cellular toxicity. Biotin and SA-FasL persisted on the cell surface for weeks in vitro and in vivo. Immunomodulation with SA-FasL-decorated splenocytes effectively blocked alloreactive responses in naive and presensitized rodents and prevented the rejection of allogeneic pancreatic islets. This approach may serve as an alternative to gene transfer-based expression with broad research and therapeutic applications.
authors.affiliation: Institute for Cellular Therapeutics and Department of Microbiology and Immunology, University of Louisville, Louisville, KY 40202, USA.
authors.forename: Esma S
authors.initials: ES
authors.lastname: Yolcu
authors.forename: Nadir
authors.initials: N
authors.lastname: Askenasy
authors.forename: Narendra P
authors.initials: NP
authors.lastname: Singh
authors.forename: Salah-Eddine Lamhamedi
authors.initials: SE
authors.lastname: Cherradi
authors.forename: Haval
authors.initials: H
authors.lastname: Shirwan
mesh_terms: D000818:Animals; D016525:Antigenic Modulation / Q000235:genetics / Q000276:immunology*; D002462:Cell Membrane / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D053222:Fas Ligand Protein; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control; D016381:Islets of Langerhans Transplantation / Q000276:immunology; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins