A method to obtain axenic Angiostrongylus vasorum first-stage larvae from dog feces.
An improved method to obtain a large number of axenic larvae of Angiostrongylus vasorum from fecal samples was developed in the present study. The procedure here in reported consisted of obtaining larvae using a modified Baermann technique, followed by an additional filtration step. This isolation technique recovered almost 90% of the living larvae in a clean preparation. Isolated larvae were submitted to decontamination treatments with either sodium hypochlorite or antibiotic cocktail solutions. The axenic status, as confirmed by oral inoculation of decontaminated larvae into germ-free mice, was only achieved using larvae treated with 0.5% sodium hypochlorite solution for 10 min. The isolation and decontamination treatment did not affect larval viability. Treated larvae remained viable and infective to the invertebrate host.
Barçante
J M P
JM
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos 6627, Campus Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil.
Barçante
T A
TA
Dias
S R C
SR
Vieira
L Q
LQ
Lima
W S
WS
Negrão-Corrêa
D
D
D000801:Angiostrongylus / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D000818:Animals; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D005243:Feces / Q000469:parasitology*; D005260:Female; D005374:Filtration; D005856:Germ-Free Life; D007814:Larva; D051379:Mice; D012973:Sodium Hypochlorite / Q000494:pharmacology
D012973:Sodium Hypochlorite