[Expression mechanisms of inducible type nitric oxide synthasegene via interleukin-1 receptor signaling].
The functional study on NF-kB bindings sites in transcription-regulatory region of rat inducible type nitric oxide synthase (iNOS) has been carried out by reporter assay to learn the signaling mechanisms of actions of interleukin-1 (IL-1). By progressive excision of 5'-ends of the insert and introduction of mutagenesis of each NF-kB binding site, it has been shown that 3 kB sites are essential for IL-1-induced signaling pathway in a mutually dependent manner.
Hisayama
Tetsuhiro
T
Department of Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokushima, Tokushima 770-8505, Japan.
Shinohara
Yasuyuki
Y
Fukui
Hiroyuki
H
D000818:Animals; D001665:Binding Sites / Q000502:physiology; D002478:Cells, Cultured; D015870:Gene Expression; D018836:Inflammation Mediators / Q000502:physiology*; D007375:Interleukin-1 / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000201:enzymology; D016328:NF-kappa B / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011493:Protein Kinase C / Q000502:physiology; D051381:Rats; D015398:Signal Transduction / Q000502:physiology*; D015533:Transcriptional Activation
D018836:Inflammation Mediators; D007375:Interleukin-1; D016328:NF-kappa B; D019001:Nitric Oxide Synthase; D011493:Protein Kinase C