Hypothesis: the RNase-sensitive restraint to unfolding of spermidine nucleoids from Escherichia coli is composed of cotranslational insertion linkages.
The genomic DNA of bacteria is highly localized in one or a few bodies known as nucleoids. A number of restraints to the unfolding of the DNA of spermidine nucleoids from Escherichia coli were previously associated with characteristic urea concentrations (U(m) values). The dominant restraint to unfolding was sensitive to pancreatic RNase and underwent a cooperative transition at U(m) = 3.2 M urea. The losses of the RNase-sensitive restraint caused by urea or pancreatic RNase appear to result from breakage of cotranslational insertion linkages which joined the nucleoid to the cell envelope in growing cells. This conclusion is based upon effects from exposures of cells to antibiotics (chloramphenicol, rifampicin, streptomycin), treatment of nucleoid preparations with formaldehyde or concentrated NaCl solutions, and effects of urea on purified ribosomes. The specific RNase-sensitive and urea-sensitive components of the spermidine nucleoids are suggested to be the mRNA and ribosomes, respectively, of cotranslational insertion linkages.
Murphy
Lizabeth D
LD
Laboratory of Molecular Biology, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0560, USA.
Zimmerman
Steven B
SB
D004926:Escherichia coli / Q000378:metabolism*; D005557:Formaldehyde / Q000737:chemistry; D014176:Protein Biosynthesis; D012260:Ribonucleases / Q000378:metabolism*; D012270:Ribosomes / Q000737:chemistry; D013095:Spermidine / Q000378:metabolism*; D014508:Urea / Q000737:chemistry
D005557:Formaldehyde; D014508:Urea; D012260:Ribonucleases; D013095:Spermidine