Differentiation of human gamma-delta T cells towards distinct memory phenotypes.
Vgamma9/Vdelta2 T cells comprise a small population of peripheral T cells responding towards the low molecular weight antigen, (E)-4-hydroxy-3-methyl-but-2-enyl-pyrophosphate (HMB-PP). HMB-PP-stimulated Vgamma9/Vdelta2 T cells proliferated, expressed CCL5/RANTES, and upregulated markers like CD16, CD25, CD69, and CD94, in the presence of either IL-15 or IL-21. Vgamma9/Vdelta2 T cells grown in the presence of IL-15 differentiated into an effector/memory population characterized by production of TNF-alpha, expression of CD45RO and CCR5, and lack of CD62L, CD81, and CCR7. In contrast, Vgamma9/Vdelta2 T cells grown with IL-21 differentiated into putative central memory CD45RO(+) T cells that did not produce TNF-alpha, IFN-gamma, or IL-4, and maintained expression of CD62L, CD81, and CCR7.
Eberl
Matthias
M
Biochemisches Institut, Justus-Liebig-Universität Giessen, Friedrichstr. 24, 35392 Giessen, Germany. matthias.eberl@biochemie.med.uni-giessen.de
Engel
Rosel
R
Beck
Ewald
E
Jomaa
Hassan
H
D015703:Antigens, CD / Q000096:biosynthesis*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured / Q000166:cytology / Q000276:immunology; D018946:Chemokine CCL5 / Q000096:biosynthesis; D011756:Diphosphates / Q000276:immunology; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology*; D018836:Inflammation Mediators / Q000378:metabolism; D019409:Interleukin-15 / Q000494:pharmacology*; D007378:Interleukins / Q000494:pharmacology*; D037181:Lectins, C-Type / Q000096:biosynthesis; D051920:NK Cell Lectin-Like Receptor Subfamily D; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D017452:Receptors, IgG / Q000096:biosynthesis; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*; D000098546:Interleukin-21
C443995:4-hydroxy-3-methylbut-2-enyl pyrophosphate; D015703:Antigens, CD; D018946:Chemokine CCL5; D011756:Diphosphates; D018836:Inflammation Mediators; D019409:Interleukin-15; D007378:Interleukins; C497869:KLRD1 protein, human; D037181:Lectins, C-Type; D051920:NK Cell Lectin-Like Receptor Subfamily D; D016692:Receptors, Antigen, T-Cell, gamma-delta; D017452:Receptors, IgG; D000098546:Interleukin-21