title: A DNA probe specific for Aeromonas colonies.
abstract: Members of the genus Aeromonas are important enteropathogens. Commercial identification systems are often unable to correctly identify Aeromonas strains and misidentification as Vibrio spp. is common. A digoxigenin-DNA probe based on a 237 bp of the glycerophospholipid-cholesterol acyltransferase gene has been tested in a colony hybridization assay. The probe hybridized with all Aeromonas species tested (n = 16) but not with strains of other enteropathogenic bacteria (n = 20). The probe allowed the unequivocal identification of Aeromonas in primary isolation media within 36 h.
authors.affiliation: Departamento de Ciencias Médicas Básicas, Facultad de Medicina y Ciencias de la Salud, Universidad Rovira y Virgili, San Lorenzo 21, 43201, Reus, Spain.
authors.forename: M R
authors.initials: MR
authors.lastname: Chacón
authors.forename: G
authors.initials: G
authors.lastname: Castro-Escarpulli
authors.forename: L
authors.initials: L
authors.lastname: Soler
authors.forename: J
authors.initials: J
authors.lastname: Guarro
authors.forename: M J
authors.initials: MJ
authors.lastname: Figueras
mesh_terms: D000217:Acyltransferases / Q000235:genetics*; D000333:Aeromonas / Q000145:classification* / Q000235:genetics / Q000254:growth & development*; D000818:Animals; D003470:Culture Media; D015342:DNA Probes; D004076:Digoxigenin; D006168:Guinea Pigs; D006801:Humans; D008049:Lipase / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D003470:Culture Media; D015342:DNA Probes; D000217:Acyltransferases; C085461:Aeromonas hydrophilia lipase-acyltransferase; D008049:Lipase; D004076:Digoxigenin