title: [Study on plasma coagulation factor VII (FVII) levels and polymorphisms of FVII gene in patients with coronary heart disease].
abstract: OBJECTIVE
  To investigate the plasma levels of coagulation factor VII (FVII) and polymorphisms of FVII gene in patients with coronary heart disease (CHD), and evaluate the effect of plasma FVII levels and FVII gene polymorphisms on CHD.
  
  
  METHODS
  Plasma FVIIa, FVII: Ag and FVIIc were measured and polymorphisms of FVII gene were analyzed in 149 control cases and 60 CHD cases, including 33 acute myocardial infarction (AMI) cases by a combination of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and agarose gel electrophoresis.
  
  
  RESULTS
  FVIIa, FVIIc in AMI group were significantly higher than that in control group, but FVII: Ag wasn't. There were no significant difference in plasma FVIIa, FVII: Ag and FVIIc between CHD and control group. The IVS7 genotypic frequency in AMI group was significantly different from that in control group. There was no significant difference in genotypic frequencies and allelic frequencies in other polymphism sites. FVII: Ag was significantly higher in -402A homozygote than that in -402G homozygote.
  
  
  CONCLUSIONS
  Increased FVII levels, especially FVIIa and FVIIc in plasma, may contribute to coronary artery thrombosis. There was significant difference in IVS7 genotype frequency between control and AMI groups, but the rest weren't significantly different. FVII: Ag was significantly higher in -402A homozygote than that in -402G homozygote. Polymorphism of -402 G/A may play an indirect role in AMI by regulating plasma FVII levels.
authors.affiliation: Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
authors.forename: Wenying
authors.initials: W
authors.lastname: Kang
authors.forename: Hongli
authors.initials: H
authors.lastname: Wang
authors.forename: Lifan
authors.initials: L
authors.lastname: Xiong
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Chu
authors.forename: Bin
authors.initials: B
authors.lastname: Qu
authors.forename: Xiangfan
authors.initials: X
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
authors.forename: Baohua
authors.initials: B
authors.lastname: Duan
authors.forename: Jinde
authors.initials: J
authors.lastname: Yu
authors.forename: Zhenyi
authors.initials: Z
authors.lastname: Wang
mesh_terms: D003327:Coronary Disease / Q000097:blood* / Q000235:genetics; D005167:Factor VII / Q000032:analysis* / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D005167:Factor VII