Middle ear instillation of gentamicin and streptomycin in chinchillas: electrophysiological appraisal of selective ototoxicity.
The purpose of this study was to investigate selective vestibular ototoxicity of gentamicin and streptomycin in the chinchilla model. In total, 10 chinchillas underwent left middle ear instillation of one of three agents: gentamicin, streptomycin and saline. Electrophysiological data (otoacoustic emissions (OAEs), auditory brainstem evoked response (ABRs), and ice-water electronystagmography were recorded before and after instillation. Animals were sacrificed for temporal bone studies using scanning electron microscopy. Morphological changes in the cochlear and vestibular neuroepithelia were correlated with electrophysiological changes. Widespread ipsilateral cochlear and vestibular neuroepithelial injuries were observed and correlated with loss of OAEs, ABRs and ice-water caloric response. This study provides no evidence of selective vestibular ototoxicity of gentamicin or streptomycin. Morphological damage correlates with, but precedes loss of electrophysiological parameters. Chinchillas, like other small mammals, may not be an ideal model for the study of human ototoxicity.
Hilton
M
M
Department of Otorhinolaryngology, Sunnybrook and Women's College Health Science Centre, University of Toronto, Canada. mhilton@doctors.org.uk
Chen
J
J
Kakigi
A
A
Hirakawa
H
H
Mount
R J
RJ
Harrison
R V
RV
D000818:Animals; D002682:Chinchilla; D003051:Cochlea / Q000648:ultrastructure; D004195:Disease Models, Animal; D004432:Ear, Middle; D004584:Electronystagmography / Q000187:drug effects; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects; D005260:Female; D005839:Gentamicins / Q000633:toxicity*; D007322:Instillation, Drug; D008854:Microscopy, Electron; D017084:Otoacoustic Emissions, Spontaneous / Q000187:drug effects; D013307:Streptomycin / Q000633:toxicity*; D014722:Vestibule, Labyrinth / Q000187:drug effects* / Q000648:ultrastructure
D005839:Gentamicins; D013307:Streptomycin