Pooled human albumin primes neutrophils.
Human pooled albumin has traditionally been used both to pacify the artificial surfaces in a cardiopulmonary bypass circuit and also for volume repletion following surgery. In evaluating the routine use of albumin in multiple phases of cardiac surgery, conscientious surgical teams must assess both the physiological and financial price of albumin. Albumin indiscriminately binds many plasma proteins and lipids. In this series of experiments, we explored the influence of highly purified albumin devoid of bound lipids and globulins on both receptor-dependent (FMLP) and receptor-independent (PMA) priming/activation of human neutrophils. We believe that it is important to distinguish the direct influence of albumin from the albumin-bound proteins and lipids. We, therefore, also examined the effect of clinically accessible human pooled albumin on human neutrophils. We observed a dose-dependent priming/activation (elastase release) of human neutrophils by both pooled and purified albumin. We conclude that it is increasingly difficult to justify the routine use of albumin in cardiac surgical patients.
Rabaglia
Jennifer L
JL
Department of Surgery, University of Colorado School of Medicine, Denver, Colorado 80262, USA.
Gonzalez
Ricardo
R
Moore
Ernest E
EE
Harken
Alden H
AH
D015415:Biomarkers / Q000097:blood; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D004305:Dose-Response Relationship, Drug; D006801:Humans; D019272:Leukocyte Elastase / Q000187:drug effects / Q000502:physiology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000008:administration & dosage / Q000494:pharmacology; D018375:Neutrophil Activation / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology*; D011493:Protein Kinase C / Q000008:administration & dosage / Q000494:pharmacology; D011955:Receptors, Drug / Q000008:administration & dosage; D012016:Reference Values; D012709:Serum Albumin / Q000008:administration & dosage / Q000494:pharmacology*
D015415:Biomarkers; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D011955:Receptors, Drug; D012709:Serum Albumin; C083141:phorbol ester binding protein; C027776:phorbol ester receptor; D009240:N-Formylmethionine Leucyl-Phenylalanine; D011493:Protein Kinase C; D019272:Leukocyte Elastase