title: Genetic variation and immunohistochemical differences among geographic isolates of Taura syndrome virus of penaeid shrimp.
abstract: Taura syndrome virus (TSV) is an important virus infecting penaeid shrimp in the western hemisphere. Genetic variation and immunohistochemical differences of 20 TSV isolates collected from the USA, Taiwan, Mexico and Nicaragua were compared. Capsid protein genes CP1 (546 bp) and CP2 (584 bp) were amplified by RT-PCR and the cDNAs were sequenced. Pairwise comparison of nucleotide sequences showed a 0-2.4% difference in CP1 and a 0-3.5% difference in CP2. Phylogenetic analyses clustered the TSV isolates into two groups: one contained USA, Taiwan and some Mexican isolates, the other contained Mexican isolates only. Immunohistochemical analysis using a TSV-specific monoclonal antibody produced positive results for the USA and Taiwan isolates but negative results for the Mexican and Nicaraguan isolates. Molecular and immunohistochemical data suggest the existence of at least two TSV strains, one of which might have evolved following contact with a new penaeid host, Penaeus stylirostris.
authors.affiliation: Department of Biological Sciences, California State University, 333 South Twin Oaks Valley Road, San Marcos, California 92096, USA1.
authors.forename: Refugio
authors.initials: R
authors.lastname: Robles-Sikisaka
authors.affiliation: Super Shrimp Inc., 1545 Tidelands Avenue, Suite J, National City, California 91950, USA2.
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Hasson
authors.affiliation: Department of Biological Sciences, California State University, 333 South Twin Oaks Valley Road, San Marcos, California 92096, USA1.
authors.forename: Denise K
authors.initials: DK
authors.lastname: Garcia
authors.affiliation: Super Shrimp Inc., 1545 Tidelands Avenue, Suite J, National City, California 91950, USA2.
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Brovont
authors.affiliation: Super Shrimp Inc., 1545 Tidelands Avenue, Suite J, National City, California 91950, USA2.
authors.forename: Karyn D
authors.initials: KD
authors.lastname: Cleveland
authors.affiliation: Super Shrimp Inc., 1545 Tidelands Avenue, Suite J, National City, California 91950, USA2.
authors.forename: Kurt R
authors.initials: KR
authors.lastname: Klimpel
authors.affiliation: Super Shrimp Inc., 1545 Tidelands Avenue, Suite J, National City, California 91950, USA2.
authors.forename: Arun K
authors.initials: AK
authors.lastname: Dhar
mesh_terms: D000569:Americas; D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014644:Genetic Variation; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000821:virology*; D010802:Phylogeny; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013624:Taiwan
chemical_list: D036022:Capsid Proteins