title: Juice and phenolic fractions of the berry Aristotelia chilensis inhibit LDL oxidation in vitro and protect human endothelial cells against oxidative stress.
abstract: Oxidative modification of low-density lipoprotein (LDL) particles is a key event in the development of atherosclerosis. Oxidized LDL induces oxidative stress and modifies gene expression in endothelial cells. Berries constitute a rich dietary source of phenolic antioxidants. We found that the endemic Chilean berry Aristotelia chilensis (ach) has higher phenol content and scores better for total radical-trapping potential and total antioxidant reactivity in in vitro antioxidant capacity tests, when compared to different commercial berries. The juice of ach is also effective in inhibiting copper-induced LDL oxidation. In human endothelial cell cultures, the addition of ach juice significantly protects from hydrogen peroxide-induced intracellular oxidative stress and is dose-dependent. The aqueous, anthocyanin-rich fraction of ach juice accounts for most of ach's antioxidant properties. These results show that ach is a rich source of phenolics with high antioxidant capacity and suggest that it may have antiatherogenic properties.
authors.affiliation: Programa Bases Moleculares de las Enfermedades Crónicas, Facultad de Ciencias Biológicas, Pontifica Universidad Católica de Chile, Santiago, Chile.
authors.forename: Soledad
authors.initials: S
authors.lastname: Miranda-Rottmann
authors.forename: Augusto A
authors.initials: AA
authors.lastname: Aspillaga
authors.forename: Druso D
authors.initials: DD
authors.lastname: Pérez
authors.forename: Luis
authors.initials: L
authors.lastname: Vasquez
authors.forename: Alvaro L F
authors.initials: AL
authors.lastname: Martinez
authors.forename: Federico
authors.initials: F
authors.lastname: Leighton
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D003300:Copper / Q000737:chemistry; D004730:Endothelium, Vascular / Q000187:drug effects*; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000737:chemistry*; D019684:Magnoliopsida / Q000737:chemistry*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry; D018514:Plant Structures / Q000737:chemistry; D014471:Umbilical Veins; D014920:Wine / Q000032:analysis
chemical_list: D000975:Antioxidants; D008077:Lipoproteins, LDL; D010636:Phenols; D010936:Plant Extracts; D003300:Copper; D006861:Hydrogen Peroxide