Biosynthesis of aminoglycoside antibiotics: cloning, expression and characterisation of an aminotransferase involved in the pathway to 2-deoxystreptamine.
The gene btrR from Bacillus circulans has been cloned and expressed and shown to produce a protein which catalyses the transamination of 2-deoxy-scyllo-inosose to give 2-deoxy-scyllo-inosamine, an intermediate in the biosynthesis of 2-deoxystreptamine.
Huang
Fanglu
F
Cambridge Centre for Molecular Recognition, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK CB2 1EW.
Li
Yanyan
Y
Yu
Jinquan
J
Spencer
Jonathan B
JB
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001407:Bacillus / Q000201:enzymology; D003001:Cloning, Molecular; D016680:Genome, Bacterial; D006595:Hexosamines / Q000096:biosynthesis*; D000637:Transaminases / Q000235:genetics* / Q000378:metabolism
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D006595:Hexosamines; D000637:Transaminases; C007265:2-deoxystreptamine