Detection of specific Helicobacter pylori DNA and antigens in stool samples in dyspeptic patients and healthy subjects.
In this study stool samples from dyspeptic patients and healthy subjects were used for detection of specific Helicobacter pylori antigens and DNA by immunoenzymatic test (PPHpSA) and semi-nested PCR (ureA-PCR), respectively. The H. pylori status was estimated by invasive endoscopy-based rapid urease test and histology or noninvasive urea breath test (UBT), and by serology (ELISA, Western blot). The coincidence of H. pylori-negative invasive tests or UBT and negative antigen or DNA stool tests was very high (mean 95%). The PPHpSA results were found positive for 56% and ureA-PCR for 26% of individuals with H. pylori infection confirmed by invasive tests or UBT. The detection of specific H. pylori antigens and especially DNA in feces is not sufficient as a one-step diagnosis of H. pylori infection.
Wiśniewska
Monika
M
Department of Immunology and Infectious Biology, University of Lódź, 90-237 Lódź, Banacha 12, Poland.
Nilsson
Hans Olof
HO
Bak-Romaniszyn
Leokadia
L
Rechciński
Tomasz
T
Bielański
Władysław
W
Płaneta-Małecka
Izabela
I
Płonka
Małgorzata
M
Konturek
Stanisław
S
Wadström
Torkel
T
Rudnicka
Wiesława
W
Chmiela
Magdalena
M
D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000032:analysis*; D015153:Blotting, Western; D001944:Breath Tests; D002648:Child; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004415:Dyspepsia / Q000175:diagnosis / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology*; D016481:Helicobacter Infections / Q000175:diagnosis / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004269:DNA, Bacterial