title: Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines.
abstract: Platelet-activating factor receptor (PAF-R) transcripts were analysed by reverse transcriptase-polymerase chain reaction in five human cancer cell lines derived from the breast (BT20, SKBR3 and T47D cells), the pancreas (Miapaca cells) and the bladder (5,637 cells) in order to confirm the existence of a splice variant of the PAF-R transcript 2. After cloning and sequencing, we confirmed its existence in all cell lines. It consisted of the PAF-R transcript 2 lengthening with 82 nucleotides from the 3' end of exon 1 of the PAF-R gene. The role of this elongated form of the tissue-type PAF-R transcript in cell physiology remains to be elucidated.
authors.affiliation: Laboratoire d'Immunologie-UMR CNRS 6101, Faculté de Médecine, 2 rue du Docteur Marcland, 87025 Limoges, France.
authors.forename: Ibtissam
authors.initials: I
authors.lastname: Youlyouz
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Magnoux
authors.forename: Laurence
authors.initials: L
authors.lastname: Guglielmi
authors.forename: Yves
authors.initials: Y
authors.lastname: Denizot
mesh_terms: D001943:Breast Neoplasms; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology*; D006801:Humans; D010190:Pancreatic Neoplasms; D010980:Platelet Membrane Glycoproteins / Q000235:genetics*; D012326:RNA Splicing / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms
chemical_list: D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor