title: 1,25-Dihydroxyvitamin D(3) increases striatal GDNF mRNA and protein expression in adult rats.
abstract: Glial cell line-derived neurotrophic factor (GDNF) has been postulated as a possible candidate for therapeutic treatment in Parkinson's disease (PD). Recent in vitro data suggest that 1,25-dihydroxyvitamin D3 [1,25(OH)(2)D(3)] treatment may enhance GDNF mRNA expression. In the present study, using semiquantitative RT-PCR and Western blot, we have shown that 1,25(OH)(2)D(3) administration intraperitoneally, significantly increases GDNF mRNA and protein levels in the striatum of adult rats.
authors.affiliation: Department of Physiology, School of Medicine, University of Santiago de Compostela, Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Sanchez
authors.forename: Elena
authors.initials: E
authors.lastname: Lopez-Martin
authors.forename: Carmen
authors.initials: C
authors.lastname: Segura
authors.forename: Jose L
authors.initials: JL
authors.lastname: Labandeira-Garcia
authors.forename: Roman
authors.initials: R
authors.lastname: Perez-Fernandez
mesh_terms: D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002120:Calcium Channel Agonists / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects* / Q000502:physiology; D051100:Glial Cell Line-Derived Neurotrophic Factor; D006801:Humans; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D010300:Parkinson Disease / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic
chemical_list: D002120:Calcium Channel Agonists; C495039:GDNF protein, human; C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D002117:Calcitriol