New label enzymes for bioluminescent enzyme immunoassay.
Firefly luciferase catalyzes the oxidation of luciferin in the presence of ATP, magnesium ion and molecular oxygen with a high quantum yield. Due to its high sensitivity and specificity for ATP, luciferase has been used for bioluminescent detection of ATP in various biological samples. But it is not known well to apply the detection of immunoassay. In this article, the use of various enzymes as labels in the design and development of immunoassays for biomolecules has been reviewed.
Maeda
Masako
M
School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. maedam@pharm.showa-u.ac.jp
D000818:Animals; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D008164:Luminescent Proteins / Q000032:analysis* / Q000494:pharmacology
D008164:Luminescent Proteins