[A novel member of SH(2) signaling protein family: cloning and characterization of SH(2)A gene].
OBJECTIVE
Cloning and characterization of a novel gene by exon trapping and exon linking at chromosome 8p22.


METHODS
A novel gene was cloned using exon trapping and exon linking, and its expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot.


RESULTS
A sequence containing 3 exons was found. The sequence is homologous with the putative gene AK024799 which consists of 2880 bp cDNA with 1362 bp open reading frame and codes 454 amino acids with an SH(2) domain. The gene was named SH(2)A at chromosome 8p22. SH(2)A gene is ubiquitously expressed in various tissues with three transcripts. The aberrant expression of SH(2)A gene in some cancers was detected.


CONCLUSIONS
SH(2)A is a novel docking protein of SH(2) signaling protein family, which may play an important role in cellular signal transduction. It relates to the pathogenesis of tumor.
Dai
Shuping
S
Department of Medical Genetics, China Medical University, Shenyang, Liaoning, 110001 P. R. China.
Zhao
Yanyan
Y
Ding
Qian
Q
D000818:Animals; D015152:Blotting, Northern; D019556:COS Cells; D002352:Carrier Proteins; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005091:Exons; D020690:GTPase-Activating Proteins; D015870:Gene Expression; D005796:Genes / Q000235:genetics; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D007438:Introns; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D020830:rac1 GTP-Binding Protein; D018909:src Homology Domains / Q000235:genetics*
D002352:Carrier Proteins; D018076:DNA, Complementary; C402110:DOCK2 protein, human; D020690:GTPase-Activating Proteins; D020662:Guanine Nucleotide Exchange Factors; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C474507:SH2D4A protein, human; D012313:RNA; D020830:rac1 GTP-Binding Protein