title: Stability of ingested methylcellulose in the rat determined by polymer molar mass measurements by light scattering.
abstract: Methylcellulose (MC) is ingested by humans in food and pharmaceutical formulations. The functional properties of MC like those of other linear polymers depend primarily on polymer length or molar mass for largely linear polymers. Although many studies in animals and humans have shown complete excretion of MC, in vitro human fecal fermentation studies indicate that MC can be degraded and presumably lose some of its functionality. In this study, MC polymer distribution in the feces from rats fed a diet containing 8% methylcellulose were compared to the fed MC. The water-soluble polymers in the feces were separated by a size exclusion chromatography (SEC) and the polymer distributions determined by multiple angle laser light scattering (MALLS). Detection of the fluorescent MC-calcofluor complex was used to confirm the identity of the eluting MC peak. All dietary MC was recovered in the feces. There is a small shift (P < 0.06) in the weight-averaged molecular weight of polymer distribution of MC extracted from the feces to 2.71 +/- 0.15 x 10(5) g/mol from 3.15 +/- 0.02 x 10(5) g/mol in the standard. There is also an increase in the polydispersity from 1.21 in the standard to 1.8 in the fecal extract. The distribution of the substituted methoxylated glucose monomers by gas chromatography also confirms the stability of MC fed to rats. The amount of actual hydrolysis is estimated to be about 0.1 glycosidic linkage/molecule. MC is not easily determined by standard dietary fiber methods, and SEC with MALLS and/or fluorescence may be a useful alternative.
authors.affiliation: Western Regional Research Center, ARS, USDA, 800 Buchanan Street, Albany, California 94710, USA. wally@pw.usda.gov
authors.forename: Wallace H
authors.initials: WH
authors.lastname: Yokoyama
authors.forename: Benny E
authors.initials: BE
authors.lastname: Knuckles
authors.forename: Paul A
authors.initials: PA
authors.lastname: Davis
authors.forename: Bruce P
authors.initials: BP
authors.lastname: Daggy
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004043:Dietary Fiber / Q000008:administration & dosage; D004355:Drug Stability; D005243:Feces / Q000737:chemistry; D006868:Hydrolysis; D008027:Light; D008297:Male; D008747:Methylcellulose / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism; D008970:Molecular Weight; D011108:Polymers / Q000032:analysis*; D051381:Rats; D011916:Rats, Inbred F344; D012542:Scattering, Radiation
chemical_list: D004043:Dietary Fiber; D011108:Polymers; D008747:Methylcellulose