title: Extracellular matrix maturation in the left ventricle of normal and diabetic swine.
abstract: The main objective of this study is to determine the transmural distribution of extracellular matrix (ECM) collagen and maturation in non-diabetic and diabetic hearts. The Yucatan miniature swine heart ECM was analyzed in eight streptozotocin (STZ) induced diabetic pigs (Diabetic-Swine) and age matched normal control pigs (Nondiabetic-Swine). After 12 weeks of STZ induced diabetes, transmural biopsies were obtained from the left ventricular free wall divided into subendocardial, mid- and subepicardial layers. Collagen concentration and maturation were measured by RP-HPLC determination of hydroxyproline (Hyp) and content of hydroxylysylpyridinoline (HP) cross-links, respectively. Results showed a significant elevation in arterial glucose (P<0.05) and reduction in arterial plasma insulin levels in the Diabetic-Swine. Hyp concentration was significantly greater (P<0.05) in the subendocardial layers in both the Diabetic and Nondiabetic animals. The HP cross-link content was significantly greater (17%) in the Diabetic-swine subendocardial layer compared to Nondiabetic-Swine (P<0.05), but not in other layers. In summary, the accumulation and/or increase in HP cross-link content in the Diabetic-Swine subendocardial layer suggests that myocardial fibrosis may be greater in this specific region.
authors.affiliation: Department of Biology and Biochemistry, University of Houston, 369 Science and Research Building II, Houston, TX 77204-5001, USA. ddam@uh.edu
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Martinez
authors.forename: David J
authors.initials: DJ
authors.lastname: Guhl
authors.forename: William C
authors.initials: WC
authors.lastname: Stanley
authors.forename: Arthur C
authors.initials: AC
authors.lastname: Vailas
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism*; D006909:Hydroxyproline / Q000378:metabolism; D007098:Imino Acids / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D013552:Swine; D013556:Swine, Miniature; D014018:Tissue Distribution; D016277:Ventricular Function, Left
chemical_list: D000596:Amino Acids; D007098:Imino Acids; C015484:pyridinoline; D003094:Collagen; D006909:Hydroxyproline