title: Analysis of microsatellite polymorphism around the HLA-B locus in Iranian patients with Behçet's disease.
abstract: We have previously suggested that in a Japanese population the susceptible locus for Behçet's disease (BD) is HLA-B51 itself. To confirm this finding in another population, we performed HLA class I typing using the PCR-SSP method and analyzed eight polymorphic markers distributed within 1100 kb around the HLA-B gene using automated sequencer and subsequent automated fragment detection by fluorescent-based technology with the DNA samples of 84 Iranian patients with BD and 87 healthy ethnically matched controls. As a result, three microsatellite alleles (MICA-A6, MIB-348, C1-4-1-217) and HLA-B51 were found to be strongly associated with BD. Of these alleles HLA-B51 is the most strongly associated allele. There were no alleles that were increased in allele frequency at any microsatellite loci centromeric of MICA or telomeric of HLA-B51. Therefore, HLA-B51 was confirmed to be by far the most strongly associated gene with BD in an Iranian population.
authors.affiliation: Department of Ophthalmology, Yokohama City University School of Medicine, Kanagawa, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Mizuki
authors.forename: K
authors.initials: K
authors.lastname: Yabuki
authors.forename: M
authors.initials: M
authors.lastname: Ota
authors.forename: Y
authors.initials: Y
authors.lastname: Katsuyama
authors.forename: H
authors.initials: H
authors.lastname: Ando
authors.forename: E
authors.initials: E
authors.lastname: Nomura
authors.forename: K
authors.initials: K
authors.lastname: Funakoshi
authors.forename: F
authors.initials: F
authors.lastname: Davatchi
authors.forename: H
authors.initials: H
authors.lastname: Chams
authors.forename: B
authors.initials: B
authors.lastname: Nikbin
authors.forename: A A
authors.initials: AA
authors.lastname: Ghaderi
authors.forename: S
authors.initials: S
authors.lastname: Ohno
authors.forename: H
authors.initials: H
authors.lastname: Inoko
mesh_terms: D001528:Behcet Syndrome / Q000235:genetics* / Q000276:immunology; D002874:Chromosome Mapping; D015235:HLA-B Antigens / Q000235:genetics*; D059949:HLA-B51 Antigen; D006801:Humans; D007492:Iran; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic
chemical_list: D015235:HLA-B Antigens; D059949:HLA-B51 Antigen