[Effects of periodontitis and opsonized zymosan on chemiluminescence of blood neutrophils].
The objective of the present investigation was to explore oxidative function of parodontitis patient's blood neutrophils stimulated with opsonized zymosan by method of luminol- and lucigenin-dependent chemiluminescence. The leukocytes for this study were obtained from peripheral venous blood of 16 parodontitis patients and 10 healthy subjects. The luminol-dependent chemiluminescence of stimulated neutrophils of parodontitis patients did not differ (p > 0.05) from control subjects (66,849 +/- 6372 cpm and 61,243 +/- 5240 cpm, respectively). Lucigenin-dependent chemiluminescence of stimulated neutrophils of parodontitis patients was increased (p < or = 0.001) comparing with control subjects (1361 +/- 169 cpm and 492 +/- 56 cpm respectively). The luminol- and lucigenin-dependent chemiluminescence (381 +/- 63 cpm, and 389 +/- 52 cpm, respectively) of nonstimulated neutrophils of parodontitis patients was significantly higher (p < or = 0.001) than analogous Cl (134 +/- 22 cpm and 138 +/- 16 cpm respectively) of control subjects. The results indicate that increased oxidative function of neutrophils of parodontitis patients possibly can affect parodontal health.
Zekonis
Gediminas
G
Kauno medicinos universiteto Ortopedines stomatologijos klinika, Sukileliu 51, 3007 Kaunas. zekonisg@hotmail.com
Ivanauskiene
Egle
E
D000293:Adolescent; D000328:Adult; D010520:Aggressive Periodontitis / Q000097:blood / Q000276:immunology; D005260:Female; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*; D009895:Opsonin Proteins / Q000494:pharmacology; D010518:Periodontitis / Q000097:blood* / Q000276:immunology; D015054:Zymosan / Q000494:pharmacology
D009895:Opsonin Proteins; D015054:Zymosan