Comparison of partial tuf gene sequences for the identification of lactobacilli.
Comparative analysis of partial tuf sequences was evaluated for the identification and differentiation of lactobacilli. Comparison of the amino acid sequences allowed differentiation between species and also between the subspecies of Lactobacillus delbrueckii. The nucleotide sequence comparison allowed differentiation between other subspecies and between some strains. Lactobacilli from several collections and isolates from dairy samples were clearly identified by comparison of short tuf sequences with those of the type strains. In evaluating the taxonomy of the Lactobacillus casei-related taxa, different tuf amino acid signatures are in favour of a classification into three distinct species. The type strain designation for the L. casei species is discussed.
Chavagnat
Frederic
F
Department of Microbiology, Swiss Dairy Research Institute, Liebefeld, CH-3003 Bern, Switzerland.
Haueter
Monika
M
Jimeno
Juan
J
Casey
Michael G
MG
D001426:Bacterial Proteins; D001483:Base Sequence; D017931:DNA Primers / Q000032:analysis; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D009154:Mutation; D010444:Peptide Elongation Factor Tu / Q000235:genetics*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010444:Peptide Elongation Factor Tu