title: Helicobacter species ribosomal DNA recovered from the liver tissue of chinese patients with primary hepatocellular carcinoma.
abstract: Using polymerase chain reaction (PCR), we examined 16S ribosomal DNA (rDNA) of Helicobacter species in liver tissue specimens obtained from 15 patients with hepatocellular carcinoma. Sixty percent (9 of 15) of these specimens were found to be positive for Helicobacter species. Four 16S rDNA fragments from positive PCR samples were directly sequenced. By sequence comparison, all were found to be 99% identical to the 16S rRNA of Helicobacter pylori.
authors.affiliation: Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, Hunan, People's Republic of China. xgfan@hotmail.com
authors.forename: Xue-Gong
authors.initials: XG
authors.lastname: Fan
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Peng
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Javed
authors.initials: J
authors.lastname: Yakoob
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Wang
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Chen
mesh_terms: D000328:Adult; D044466:Asian People; D006528:Carcinoma, Hepatocellular / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis*; D005260:Female; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008113:Liver Neoplasms / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S