title: Long-term cytocompatibility of various endodontic sealers using a new root canal model.
abstract: It was the purpose of our study to determine the cytotoxicity of several types of root canal sealers in vitro over the period of 1 yr by using a new test model. Roots of extracted human teeth were filled with N2, Apexit, Roekoseal, AH Plus, Ketac Endo, Endomethasone, and one gutta-percha point. In addition, roots filled with laterally condensed gutta-percha/N2. Teeth filled with one gutta-percha point only were controls. All specimens were consecutively extracted with distilled water for a total period of 1 yr. Extracts were investigated for cytotoxicity by using immortalized 3T3 fibroblasts and primary human periodontal ligament fibroblasts. Results were statistically analyzed with Dunnett's t tests (p < 0.05). Pronounced cytotoxic effects were only caused by N2-extracts in both cell cultures (p < 0.05). Furthermore, statistically significant cytotoxic alterations were also induced by 10-week eluates of Endomethasone (p < 0.05). All other investigated materials did not significantly alter cell metabolism.
authors.affiliation: Department of Conservative Dentistry & Periodontology, Medical University Hannover, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Schwarze
authors.forename: G
authors.initials: G
authors.lastname: Leyhausen
authors.forename: W
authors.initials: W
authors.lastname: Geurtsen
mesh_terms: D016475:3T3 Cells; D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000633:toxicity; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity*; D002126:Calcium Hydroxide / Q000633:toxicity; D018929:Cell Culture Techniques; D003738:Dental Cements / Q000633:toxicity; D003907:Dexamethasone / Q000633:toxicity; D004338:Drug Combinations; D004853:Epoxy Resins / Q000633:toxicity; D005054:Eugenol / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects; D005557:Formaldehyde / Q000633:toxicity; D005899:Glass Ionomer Cements / Q000633:toxicity; D006171:Gutta-Percha / Q000633:toxicity; D006801:Humans; D006854:Hydrocortisone; D006863:Hydrogen-Ion Concentration; D051379:Mice; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000633:toxicity*; D013223:Statistics as Topic; D013943:Thymol / Q000031:analogs & derivatives* / Q000633:toxicity; D013997:Time Factors; D015034:Zinc Oxide / Q000633:toxicity
chemical_list: D000893:Anti-Inflammatory Agents; C074418:Apexit; D001672:Biocompatible Materials; D003738:Dental Cements; D004338:Drug Combinations; D004853:Epoxy Resins; D005899:Glass Ionomer Cements; C074934:Ketac Endo; D012387:Root Canal Filling Materials; C011244:corticosteroid methanetriol mixture; C534916:epoxy resin-based root canal sealer; C465381:roeko seal; D005557:Formaldehyde; D013943:Thymol; D005054:Eugenol; D003907:Dexamethasone; C003799:N2 Dental Cement; D006171:Gutta-Percha; D002126:Calcium Hydroxide; D015034:Zinc Oxide; D006854:Hydrocortisone