title: Maintenance of sexual immaturity in male mice and bucks by immunization against N-terminal peptides of the follicle-stimulating hormone receptor.
abstract: The follicle-stimulating hormone is one of the two pituitary hormones that control fertility in both sexes. In the male, receptors for FSH (FSHR) are only expressed on testicular Sertoli cells. FSH plays different roles during the male life; it functions as a growth factor during development and sustains spermatogenesis in adults. However, the exact role of this hormone as an initiator of male fertility is not fully understood and few data are available concerning its involvement during the peripubertal period. We recently produced filamentous phages displaying FSHR fragments overlapping residues 18-38, which, if injected in animals, induced anti-FSH receptor immunity capable of inhibiting hormone binding. We employed this strategy to transiently inhibit FSH activity in male mice and male goats of the Saanen and the Mongolian Alpas Cashmere breeds at the prepubertal stage. Anti-FSHR peptide immunization from the age of 3 wk delayed the acquisition of fecundity in male mice by up to 1 wk. Once fertile, progeny sizes produced by mating immunized males and untreated females were found to be reduced by up to 60%. In two different breeds of goats, FSHR peptide vaccines were able to maintain circulating testosterone at low prepubertal levels for several months despite no alteration in LH levels, reflecting their ability to delay the onset of puberty. These results support the conclusion that FSH may play a central role in the male at puberty through the control of testosterone production.
authors.affiliation: Laboratory of Molecular and Cellular Biology, I.N.R.A. Biotechnology, 78352 Jouy-en-Josas, France.
authors.forename: Latifa
authors.initials: L
authors.lastname: Abdennebi
authors.forename: E Ying
authors.initials: EY
authors.lastname: Chun
authors.forename: Hélène
authors.initials: H
authors.lastname: Jammes
authors.forename: De
authors.initials: D
authors.lastname: Wei
authors.forename: Jean Jacques
authors.initials: JJ
authors.lastname: Remy
mesh_terms: D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D005298:Fertility; D005640:Follicle Stimulating Hormone / Q000037:antagonists & inhibitors / Q000502:physiology; D006041:Goats; D006128:Growth; D007114:Immunization; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D011962:Receptors, FSH / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000276:immunology*; D012741:Sexual Maturation / Q000276:immunology* / Q000502:physiology; D013739:Testosterone / Q000096:biosynthesis; D014935:Wool / Q000254:growth & development
chemical_list: D010446:Peptide Fragments; D011962:Receptors, FSH; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone