[Expression of vascular endothelial growth factor C in human breast cancer cell lines MCF-7 and MCF-7/Adr].
OBJECTIVE
Vascular endothelial growth factor C (VEGF-C) discovered recently is a new member of vascular endothelial growth factor (VEGF) family. Studies have shown that VEGF-C is one specific regulating factor for lymphatic endothelial cells, and it expresses in a variety of human tumors. In order to explore the role of VEGF-C in the process of human tumor formation and development, the authors designed this study to examine the expressions of VEGF-C mRNA and protein in human breast cancer cell line MCF-7 and drug resistant cell line MCF-7/Adr.


METHODS
According to the VEGF-C gene sequence, A specific oligonucleotide probe labeled with digoxin was designed and constructed; Then in situ hybridization was applied to detect the expression of VEGF-C mRNA in the cultured human breast cancer cell lines MCF-7 and MCF-7/Adr respectively; Finally immunohistochemistry was performed to examine the expression of VEGF-C protein in both cell lines.


RESULTS
Blue positive staining granules for VEGF-C mRNA were observed in the cytoplasm of these cells by in situ hybridization where brown positive staining granules for VEGF-C protein were also observed by immunohistochemistry. While there were no positive staining granules in neither control cells.


CONCLUSIONS
Human breast cancer cell lines MCF-7 and MCF-7/Adr can transcribe VEGF-C mRNA and translate corresponding protein in their cytoplasm.
Gao
Jie
J
Department of Human Anatomy, Medical School, Shandong University, Jinan 250012, P. R. China. jieyuan_gx@yahoo.com.cn
Liu
Zhi-yu
ZY
Wang
Wei
W
Bi
Yu-shun
YS
Tian
Hua
H
D001943:Breast Neoplasms / Q000737:chemistry / Q000378:metabolism*; D016228:Endothelial Growth Factors / Q000032:analysis / Q000235:genetics*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured; D042582:Vascular Endothelial Growth Factor C
D016228:Endothelial Growth Factors; D012333:RNA, Messenger; D042582:Vascular Endothelial Growth Factor C