title: Induction of epithelial tumors in Drosophila melanogaster heterozygous for the tumor suppressor gene wts.
abstract: The imaginal disk cells of Drosophila have a cell cycle that is very similar to that of mammalian cells. Data concerning factors inducing tumors in these cells may directly relate to the risk of these factors for inducing cancer in humans. One of the genes involved in the regulation of cell cycle control is wts (warts), the Drosophila homolog of the mammalian tumor suppressor gene LATS1. The Drosophila wts mutations are recessive lethal. However, homozygous clones that arise in heterozygous flies in the imaginal disk cells lead to epithelial tumors, spectacular outgrowths visible on the cuticle of the adult. We have treated Drosophila larvae, heterozygous for wts, with the chemical mutagen MMS (methyl methanesulfonate) or with X-rays and measured the appearance of epithelial tumors in the eclosing adult flies. This test is a variation of the well-known Drosophila somatic mutation and recombination test (SMART), where mostly recessive markers have been used leading to visible phenotypes in the eyes and wings of the fly. We show that the sensitivity of this test is far greater than the comparable test system using the recessive eye marker white.
authors.affiliation: Department of Radiation Genetics and Chemical Mutagenesis, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Jan C J
authors.initials: JC
authors.lastname: Eeken
authors.forename: Ilse
authors.initials: I
authors.lastname: Klink
authors.forename: Bert L
authors.initials: BL
authors.lastname: van Veen
authors.forename: Albert
authors.initials: A
authors.lastname: Pastink
authors.forename: Wouter
authors.initials: W
authors.lastname: Ferro
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D003433:Crosses, Genetic; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D005260:Female; D005819:Genetic Markers; D006579:Heterozygote; D006720:Homozygote; D019656:Loss of Heterozygosity; D008297:Male; D008741:Methyl Methanesulfonate; D009152:Mutagenicity Tests; D009153:Mutagens; D009154:Mutation; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics*; D010641:Phenotype; D017956:Photoreceptor Cells, Invertebrate / Q000473:pathology; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000502:physiology; D011995:Recombination, Genetic; D014860:Warts / Q000235:genetics; D014965:X-Rays
chemical_list: D029721:Drosophila Proteins; D005819:Genetic Markers; D009153:Mutagens; D008741:Methyl Methanesulfonate; D011494:Protein Kinases; C093503:wts protein, Drosophila; D017346:Protein Serine-Threonine Kinases