title: Tequila authenticity assessment by headspace SPME-HRGC-IRMS analysis of 13C/12C and 18O/16O ratios of ethanol.
abstract: By use of headspace SPME sampling and a PLOT column, on-line capillary gas chromatography-isotope ratio mass spectrometry was employed in the combustion (C) and the pyrolysis (P) modes (HRGC-C/P-IRMS) to determine the delta(13)C(VPDB) and delta(18)O(VSMOW) values of ethanol in authentic (n = 14) and commercial tequila samples (n = 15) as well as a number of other spirits (n = 23). Whereas with delta(13)C(VPDB) values ranging from -12.1 to -13.2 per thousand and from -12.5 to -14.8 per thousand similar variations were found for 100% agave and mixed tequilas, respectively, the delta(18)O(VSMOW) data differed slightly within these categories: ranges from +22.1 to +22.8 per thousand and +20.8 to +21.7 per thousand were determined for both the authentic 100% agave and mixed products, respectively. The data recorded for commercial tequilas were less homogeneous; delta(13)C(VPDB) data from -10.6 to -13.9 per thousand and delta(18)O(VSMOW) values from +15.5 to +22.7 per thousand were determined in tequilas of both categories. Owing to overlapping data, attempts to differentiate between white, rested, and aged tequilas within each of the two categories failed. In addition, discrimination of tequila samples from other spirits by means of delta(13)C(VPDB) and delta(18)O(VSMOW) data of ethanol was restricted to the products originating from C(3) as well as C(4)/CAM raw materials.
authors.affiliation: Lehrstuhl für Lebensmittelchemie, Universität Würzburg, Am Hubland, 97074 Würzburg, Germany.
authors.forename: Blanca O
authors.initials: BO
authors.lastname: Aguilar-Cisneros
authors.forename: Mercedes G
authors.initials: MG
authors.lastname: López
authors.forename: Elke
authors.initials: E
authors.lastname: Richling
authors.forename: Frank
authors.initials: F
authors.lastname: Heckel
authors.forename: Peter
authors.initials: P
authors.lastname: Schreier
mesh_terms: D027882:Agave; D000434:Alcoholic Beverages / Q000032:analysis*; D002244:Carbon / Q000032:analysis; D002247:Carbon Isotopes / Q000032:analysis*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D000431:Ethanol / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007700:Kinetics; D010100:Oxygen / Q000032:analysis; D010103:Oxygen Isotopes / Q000032:analysis*; D011786:Quality Control
chemical_list: D002247:Carbon Isotopes; D010103:Oxygen Isotopes; D000431:Ethanol; D002244:Carbon; D010100:Oxygen