title: Apparent lack of trans-dominant negative effects of various vif mutants on the replication of HIV-1.
abstract: The vif gene of human immunodeficiency virus type 1 (HIV-1) is essential for virus growth in non-permissive cells such as H9. To elucidate the mechanism of action of the Vif protein, vif mutants, which show trans-dominant negative effects on the replication of HIV-1, would be useful tools. In this study, a new assay system to identify the mutants of this category was established. For this new system, various reporter clones carrying both mutant and authentic vif sequences were generated. By determining the growth ability of the viruses derived from the reporter constructs, the potential negative effect of the mutant vif sequence was readily and sensitively monitored. Ten vif mutant sequences tested were found not to exert the trans-dominant negative effect on the replication of HIV-1.
authors.affiliation: Department of Virology, The University of Tokushima Graduate School of Medicine, Tokushima-shi, Tokushima 770-8503, Japan. mfujita@basic.med.tokushima-u.ac.jp
authors.forename: Mikako
authors.initials: M
authors.lastname: Fujita
authors.forename: Sora
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Akiko
authors.initials: A
authors.lastname: Sakurai
authors.forename: Naoya
authors.initials: N
authors.lastname: Doi
authors.forename: Maki
authors.initials: M
authors.lastname: Miyaura
authors.forename: Akiko
authors.initials: A
authors.lastname: Yoshida
authors.forename: Akio
authors.initials: A
authors.lastname: Adachi
mesh_terms: D001681:Biological Assay; D015153:Blotting, Western; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000096:biosynthesis; D016346:Gene Products, vif / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017930:Genes, Reporter; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D009154:Mutation; D014779:Virus Replication; D054320:vif Gene Products, Human Immunodeficiency Virus
chemical_list: D004279:DNA, Viral; D016346:Gene Products, vif; D054320:vif Gene Products, Human Immunodeficiency Virus