TiO2 sol-gel derived amperometric biosensor for H2O2 on the electropolymerized phenazine methosulfate modified electrode.
A novel hydrogen peroxide biosensor was developed based on the immobilization of horseradish peroxidase (HRP) in a TiO(2) sol-gel matrix on an electropolymerized phenazine methosulfate (PMS) modified electrode surface. Such membranes are of interest due to their high surface area, biological compatibility, and ease of fabrication. HRP entrapped in the TiO(2) matix was stable and retained its activity to a large extent. Cyclic voltammetry and amperometric measurements were employed to demonstrate the feasibility of electron transfer between immobilized HRP and the glassy carbon electrode via electropolymerized PMS. The influence of various experimental parameters such as operating potential, pH, temperature, and stability was investigated for optimum analytical performance. The biosensor provided a wide linear calibration range from 4.0x10(-6) M to 1.0x10(-3) M, with a detection limit of 8.0x10(-7) M at a signal-to-noise ratio of 3. The sensor retained 80% of its original activity after two months of operation.
Xu
Xin
X
Department of Chemistry, Fudan University, Shanghai 200433, P R China.
Zhao
Jiequan
J
Jiang
Dechen
D
Kong
Jilie
J
Liu
Baohong
B
Deng
Jiaqi
J
D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D017736:Ion-Selective Electrodes; D008773:Methylphenazonium Methosulfate / Q000737:chemistry*; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*
C009495:titanium dioxide; D008773:Methylphenazonium Methosulfate; D006861:Hydrogen Peroxide; D014025:Titanium; D006735:Horseradish Peroxidase