Catecholaminergic innervation of the septum in the frog: a combined immunohistochemical and tract-tracing study.
In the present study, we have investigated the distribution and the origin of the catecholaminergic innervation of the septal region in the frog Rana perezi. Immunohistochemistry for dopamine and two enzymes required for the synthesis of catecholamines, tyrosine hydroxylase (TH) and dopamine beta-hydroxylase (DBH) revealed a complex pattern of catecholaminergic (CA) innervation in the anuran septum. Dopaminergic fibers were primarily present in the dorsal portion of the lateral septum, whereas noradrenergic (DBH immunoreactive) fibers predominated in the medial septum/diagonal band complex. Catecholaminergic cell bodies were never observed within the septum. To determine the origin of this innervation, applications of dextran amines, both under in vivo and in vitro conditions, into the septum were combined with immunohistochemistry for TH. Results from these experiments demonstrated that four catecholaminergic cell groups project to the septum: (1) the group related to the zona incerta in the ventral thalamus, (2) the posterior tubercle/mesencephalic group, (3) the locus coeruleus, and (4) the nucleus of the solitary tract. While the two first groups provide dopaminergic innervation to the septum, the locus coeruleus provides the major noradrenergic projection. Noradrenergic fibers most likely arise also in the nucleus of the solitary tract. The results obtained in Rana perezi are readily comparable to those in mammals suggesting that the role of catecholamines in the septum is well conserved through phylogeny and that the CA innervation of the amphibian septum may be involved in functional circuits similar to those in mammals.
Sánchez-Camacho
Cristina
C
Departamento de Biología Celular, Facultad de Biología, Universidad Complutense of Madrid, 28040 Madrid, Spain.
Peña
Juan J
JJ
González
Agustín
A
D000344:Afferent Pathways / Q000033:anatomy & histology* / Q000737:chemistry; D000818:Animals; D001921:Brain / Q000033:anatomy & histology; D002395:Catecholamines / Q000032:analysis; D004298:Dopamine / Q000032:analysis*; D004299:Dopamine beta-Hydroxylase / Q000032:analysis*; D007150:Immunohistochemistry; D009412:Nerve Fibers / Q000737:chemistry*; D009474:Neurons / Q000737:chemistry; D011898:Ranidae; D020665:Septum of Brain / Q000033:anatomy & histology / Q000737:chemistry*; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*
D002395:Catecholamines; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D004298:Dopamine