title: Upregulation of CRH gene expression and downregulation of arginine vasopressin gene expression in the hypothalamus of bilateral nephrectomized rats.
abstract: The expression of the corticotropin-releasing hormone (CRH) gene and the arginine vasopressin (AVP) gene in the hypothalamus examined in bilateral nephrectomized rats by in situ hybridization histochemistry. The expression of the CRH gene was significantly increased in the parvocellular part of the paraventricular nucleus (PVN) 12 and 20 h after bilateral nephrectomy in comparison with that after sham operation. The plasma concentration of adrenocorticotropic hormone (ACTH) in nephrectomized rats was significantly higher than that in sham operated rats 20 h after surgery. In contrast, the expression of the AVP gene in both the parvocellular and magnocellular parts of the PVN and throughout the supraoptic nucleus (SON) was significantly decreased 20 h after bilateral nephrectomy in comparison with that after sham operation. These results suggest that nephrectomy-induced upregulation of the CRH gene with elevation of plasma ACTH may be due to the activation of the hypothalamo-pituitary adrenal (HPA) axis.
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, 807-8555, Kitakyushu, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Nomura
authors.forename: Ryota
authors.initials: R
authors.lastname: Serino
authors.forename: Yukiyo
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Yumi
authors.initials: Y
authors.lastname: Ozaki
authors.forename: Jun
authors.initials: J
authors.lastname: Saito
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamashita
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Ueta
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D001127:Arginine Vasopressin / Q000096:biosynthesis* / Q000235:genetics*; D001806:Blood Urea Nitrogen; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis* / Q000235:genetics*; D015536:Down-Regulation / Q000502:physiology; D007031:Hypothalamus / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D009392:Nephrectomy; D015345:Oligonucleotide Probes; D010286:Paraventricular Hypothalamic Nucleus / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013495:Supraoptic Nucleus / Q000187:drug effects; D015854:Up-Regulation / Q000502:physiology
chemical_list: D015345:Oligonucleotide Probes; D001127:Arginine Vasopressin; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone