title: Hyphal tip-associated localization of Cdc42 is F-actin dependent in Candida albicans.
abstract: The rho-type GTPase Cdc42 is important for the establishment and maintenance of eukaryotic cell polarity. To examine whether Cdc42 is regulated during the yeast-to-hypha transition in Candida albicans, we constructed a green fluorescence protein (GFP)-Cdc42 fusion under the ACT1 promoter and observed its localization in live C. albicans cells. As in Saccharomyces cerevisiae, GFP-Cdc42 was observed around the entire periphery of the cell. In yeast-form cells of C. albicans, it clustered to the tips and sides of small buds as well as to the mother-daughter neck region of large-budded cells. Upon hyphal induction, GFP-Cdc42 clustered to the site of hyphal evagination and remained at the tips of the hyphae. This temporal and spatial localization of Cdc42 suggests that its activity is regulated during the yeast-to-hypha transition. In addition to the accumulation at the hyphal tip, GFP-Cdc42 was also seen as a band within the hyphal tube in cells that had undergone nuclear separation. With the F-actin-assembly inhibitor latrunculin A, we found that GFP-Cdc42 accumulation at the bud site in yeast-form cells is F-actin independent, whereas GFP-Cdc42 accumulation at the hyphal tip requires F-actin. Furthermore, disruption of the F-actin cytoskeleton impaired the transcriptional induction of hypha-specific genes. Therefore, hypha formation resembles mating in Saccharomyces cerevisiae in that both require F-actin for GFP-Cdc42 localization and efficient signaling.
authors.affiliation: Department of Biological Chemistry, University of California-Irvine, Irvine, California 92697-1700, USA.
authors.forename: Idit
authors.initials: I
authors.lastname: Hazan
authors.forename: Haoping
authors.initials: H
authors.lastname: Liu
mesh_terms: D000199:Actins / Q000378:metabolism*; D015152:Blotting, Northern; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002176:Candida albicans / Q000378:metabolism*; D002453:Cell Cycle; D003599:Cytoskeleton / Q000378:metabolism; D017931:DNA Primers / Q000494:pharmacology; D005800:Genes, Fungal; D049452:Green Fluorescent Proteins; D025301:Hyphae / Q000378:metabolism*; D008164:Luminescent Proteins / Q000378:metabolism; D008387:Marine Toxins / Q000494:pharmacology; D008957:Models, Genetic; D010957:Plasmids / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D015398:Signal Transduction; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines; D013997:Time Factors; D014158:Transcription, Genetic; D020764:cdc42 GTP-Binding Protein / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D017931:DNA Primers; D008164:Luminescent Proteins; D008387:Marine Toxins; D011993:Recombinant Fusion Proteins; D013844:Thiazoles; D053778:Thiazolidines; D049452:Green Fluorescent Proteins; D020764:cdc42 GTP-Binding Protein; C037067:latrunculin A