[The functional and phenotypic analysis of T lymphocytes under stimulation of THANK].
OBJECTIVE
To study the function of THANK in T lymphocyte immune regulation.


METHODS
On the basis of THANK gene cloning and expression, isolated T and B lymphocytes were stimulated by purified THANK protein and its function in immune regulation was investigated by (3)HTdR incorperation and flow cytometric analysis.


RESULTS
In addition to the costimulation on B cell growth, THANK promoted CD3-mediated T cells proliferation, activated resting peripheral blood T lymphocytes and induced apoptosis and the phenotypic change of activated T lymphocytes.


CONCLUSIONS
THANK is involved in the modulation of T cells phenotype and function.
Wu
Dong
D
Eastern Hepatobiliary Hospital, Shanghai, 200438 China.
Shen
Feng
F
Wu
Mengchao
M
D015703:Antigens, CD / Q000032:analysis; D053264:B-Cell Activating Factor; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D017252:CD3 Complex / Q000032:analysis; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D008565:Membrane Proteins / Q000235:genetics / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000494:pharmacology*
D015703:Antigens, CD; D053264:B-Cell Activating Factor; D017252:CD3 Complex; D008565:Membrane Proteins; D011994:Recombinant Proteins; C505719:TNFSF13B protein, human; D014409:Tumor Necrosis Factor-alpha; D014316:Tritium; D013936:Thymidine