Expression of the p53 and nm23 genes in cholesteatoma.
OBJECTIVE
To evaluate the roles of p53 and nm23 genes in cholesteatoma.


METHODS
The results of p53 and nm23 immunostainings were compared between 28 cholesteatoma samples and 13 control skin samples obtained from the external acoustic canal.


RESULTS
There were no correlations between p53 staining and either gender or type of cholesteatoma (p < 0.05). The mean numbers of p53-positive cells in the unpaired cholesteatoma and skin specimens were 23.1 +/- 43.5 and 1 +/- 1.9, respectively and this difference was not statistically significant (z = -1.531; p = 0.2). The mean numbers of p53-positive cells in the paired cholesteatoma and skin specimens were 3.8 +/- 6.1 and 29 +/- 56.9, respectively and this difference was also not statistically significant (t = -1.078; p = 0.3). Cholesteatoma showed no nm23 positivity.


CONCLUSIONS
The mechanisms relating to the expression of p53 and nm23 genes are not associated with the occurrence of cholesteatoma.
Bayazit
Yildirim A
YA
Department of Otolaryngology and Head & Neck Surgery, Faculty of Medicine, Gaziantep University, Kolejtepe, Gaziantep, Turkey. bayazity@yahoo.com
Bakir
Kemal
K
Kanlikama
Muzaffer
M
Ozer
Enver
E
Mumbuc
Semih
S
Disikirik
Ilyas
I
Ucak
Ramazan
R
D000293:Adolescent; D000328:Adult; D001706:Biopsy; D002648:Child; D018424:Cholesteatoma, Middle Ear / Q000235:genetics* / Q000378:metabolism / Q000601:surgery; D002908:Chronic Disease; D004431:Ear, External / Q000378:metabolism / Q000473:pathology; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase; D012867:Skin / Q000378:metabolism / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D054778:NM23 Nucleoside Diphosphate Kinases; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins