No effects of extremely low frequency magnetic fields found on cytotoxic activities and cytokine production of human peripheral blood mononuclear cells in vitro.
Several epidemiologic studies have suggested an association between exposure to extremely low frequency (ELF) magnetic fields (MFs) and cancer in adults and children. A possible target of MFs is the immune system. The effects of the exposure to ELF MFs on the immunological functions of human peripheral blood mononuclear cells (PBMCs) obtained from healthy male volunteers were assessed by measuring the natural killer (NK) and lymphokine activated killer (LAK) activities and the production of interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), interleukin-2 (IL-2), and interleukin-10 (IL-10). The PBMCs were exposed to three different MF: linearly polarized (vertical), circularly polarized, and elliptically polarized, at 50 and 60 Hz. Magnetic flux densities were set at 500, 100, 20, and 2 microT (rms) for vertical field and at 500 microT (rms) for the rotating fields. Using cytotoxicity assay and enzyme-linked immunosorbent assay (ELISA) for cytokine production, we could not find any effects of ELF MFs on the cytotoxic activities and the cytokines production of human PBMCs.
Ikeda
Keiichi
K
Technical Research Center, The Kansai Electric Power Company, Inc, Amagasaki, Japan.
Shinmura
Yasushi
Y
Mizoe
Hiroki
H
Yoshizawa
Hiroaki
H
Yoshida
Atsuya
A
Kanao
Seiichi
S
Sumitani
Hiroyuki
H
Hasebe
Setsu
S
Motomura
Toshio
T
Yamakawa
Taku
T
Mizuno
Fumio
F
Otaka
Yoshihisa
Y
Hirose
Hideki
H
D000328:Adult; D016207:Cytokines / Q000096:biosynthesis*; D003601:Cytotoxicity Tests, Immunologic; D004574:Electromagnetic Fields / Q000009:adverse effects*; D006801:Humans; D015979:Killer Cells, Lymphokine-Activated / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
D016207:Cytokines