Gene expression profiling using advanced mass spectrometric approaches.
In the era of systems biology, computational and high-throughput experimental biological approaches are increasingly being combined to provide global snapshots of entire genomes and proteomes under tissue- and disease-specific conditions. The aim is to identify proteins changing in concentration and/or post-translational state and/or location, and develop a better molecular level understanding of the operation of biological systems. Here we describe an approach for comparative proteomics that builds upon the combination of high-performance nano-scale separations with the high-mass measurement accuracy, mass-resolving power and sensitivity of Fourier transform ion cyclotron resonance mass spectrometry to provide broad dynamic range, comprehensive and quantitative proteome measurements.
Pasa-Tolić
Ljiljana
L
Environmental Molecular Sciences Laboratory, MSIN: K8-98, Pacific Northwest National Laboratory, PO Box 999, Richland, Washington 99352, USA. ljiljana.pasatolic@pnl.gov
Lipton
Mary S
MS
Masselon
Christophe D
CD
Anderson
Gordon A
GA
Shen
Yufeng
Y
Tolić
Nikola
N
Smith
Richard D
RD
D000595:Amino Acid Sequence; D000818:Animals; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics; D034301:Deinococcus / Q000737:chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D036103:Nanotechnology; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity
D029742:Caenorhabditis elegans Proteins; D005656:Fungal Proteins