Auxin and ethylene response interactions during Arabidopsis root hair development dissected by auxin influx modulators.
The plant hormones auxin and ethylene have been shown to play important roles during root hair development. However, cross talk between auxin and ethylene makes it difficult to understand the independent role of either hormone. To dissect their respective roles, we examined the effects of two compounds, chromosaponin I (CSI) and 1-naphthoxyacetic acid (1-NOA), on the root hair developmental process in wild-type Arabidopsis, ethylene-insensitive mutant ein2-1, and auxin influx mutants aux1-7, aux1-22, and double mutant aux1-7 ein2. Beta-glucuronidase (GUS) expression analysis in the BA-GUS transgenic line, consisting of auxin-responsive domains of PS-IAA4/5 promoter and GUS reporter, revealed that 1-NOA and CSI act as auxin uptake inhibitors in Arabidopsis roots. The frequency of root hairs in ein2-1 roots was greatly reduced in the presence of CSI or 1-NOA, suggesting that endogenous auxin plays a critical role for the root hair initiation in the absence of an ethylene response. All of these mutants showed a reduction in root hair length, however, the root hair length could be restored with a variable concentration of 1-naphthaleneacetic acid (NAA). NAA (10 nM) restored the root hair length of aux1 mutants to wild-type level, whereas 100 nM NAA was needed for ein2-1 and aux1-7 ein2 mutants. Our results suggest that insensitivity in ethylene response affects the auxin-driven root hair elongation. CSI exhibited a similar effect to 1-NOA, reducing root hair growth and the number of root hair-bearing cells in wild-type and ein2-1 roots, while stimulating these traits in aux1-7and aux1-7ein2 roots, confirming that CSI is a unique modulator of AUX1.
Rahman
Abidur
A
Graduate School of Science and Technology, Kobe University, Kobe, Japan.
Hosokawa
Satoko
S
Oono
Yutaka
Y
Amakawa
Taisaku
T
Goto
Nobuharu
N
Tsurumi
Seiji
S
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D004347:Drug Interactions; D005030:Ethylenes / Q000494:pharmacology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D005966:Glucuronidase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D006016:Glycolates / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010641:Phenotype; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D030821:Plants, Genetically Modified; D012503:Saponins / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects
D005030:Ethylenes; D006016:Glycolates; D007210:Indoleacetic Acids; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D012503:Saponins; C075945:chromosaponin I; C034182:1-naphthaleneacetic acid; C036216:ethylene; C018610:1-naphthoxyacetic acid; D005966:Glucuronidase