title: [The expression of nitric oxide synthase in testes of male rat].
abstract: OBJECTIVE
  To determine the expression of nitric oxide synthase (NOS) in testis and to investigate the effects of NO on the reproductive function of testis.
  
  
  METHODS
  Testes of adult male Sprague-Dawley rats were fixed in 4% paraformaldehyde. The paraffin sections were made as routine. Immunohistochemical ABC method was used to observe the localization of NOS.
  
  
  RESULTS
  Endothelia NOS (eNOS), neuronal NOS (nNOS) and inductive NOS (iNOS) were all expressed in Leydig cells. Only eNOS was expressed in peritubular myoid cells, endothelial and smooth muscle cells of blood vessel, while only nNOS expressed in tunica adventitia of testicular blood vessels. The reactive substance distributes in cytoplasm with negative nuclei. Immunoreactivity for eNOS, nNOS and iNOS in all spermatogenic cells was negative.
  
  
  CONCLUSIONS
  Three kinds of NOS were all expressed in testis and the distribution of different NOS had a little difference.
authors.affiliation: Department of Histology and Embryology, Nanhua University, Hengyang, Hunan 421001, China.
authors.forename: Mei-Xiang
authors.initials: MX
authors.lastname: Li
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: He
authors.forename: Zi-Qian
authors.initials: ZQ
authors.lastname: Guo
authors.forename: Yue-Shun
authors.initials: YS
authors.lastname: Liu
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Long
mesh_terms: D000818:Animals; D007150:Immunohistochemistry; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000201:enzymology*
chemical_list: D009569:Nitric Oxide; D019001:Nitric Oxide Synthase