Application of a real-time biosensor to detect bacteria in platelet concentrates.
A spore-based biosensor for detecting low levels of bacteria in real-time has been recently developed. The system (termed LEXSAS, label-free exponential signal-amplification system) exploits spore's ability to produce fluorescence when sensing neighboring bacterial cells. We studied the LEXSAS as a possible approach for identifying bacterially contaminated platelet concentrates prior to transfusion because the system offers rapid analysis, high sensitivity, and low cost. If successful, this approach could reduce the risk of morbidity and mortality from transfusion-related bacteremia and sepsis. In this study, we used the LEXSAS for detecting bacteria in platelet concentrates spiked with Bacillus cereus, Enterobacter cloacae, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, or Streptococcus pyogenes. Bacteria were separated from platelets using a 2-min procedure based on bacterial resistance to detergents and osmotic shock. The results indicate that the LEXSAS could be used to design a practical biosensor for identifying bacterially contaminated platelets in real-time.
Rotman
Boris
B
Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA. rotman@bcrbiotech.com
Cote
Mindy A
MA
D000626:Aminopeptidases; D016470:Bacteremia / Q000517:prevention & control; D001419:Bacteria / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods*; D001792:Blood Platelets / Q000382:microbiology*; D004151:Dipeptides; D005453:Fluorescence; D006801:Humans; D018805:Sepsis / Q000517:prevention & control; D013171:Spores, Bacterial; D065227:Transfusion Reaction
D004151:Dipeptides; C002956:alanylalanine; D000626:Aminopeptidases