title: Cellular signaling during the macrophage invasion by Trypanosoma cruzi.
abstract: We have reported that protein tyrosine kinases play an important role in the invasion of Trypanosoma cruzi into primary resident macrophages. In the present study we carry out immunofluorescence assays, using monoclonal anti-phosphotyrosine antibodies, to reveal an accumulation of tyrosine-phosphorylated residues at the site of parasite association with the macrophage surface, colocalizing with host cell F-actin-rich domains. SDS-PAGE analysis of macrophage cell line IC-21 tyrosine phosphoproteins, labeled with [(35)S] L-methionine, revealed several peptides with increased levels of phosphorylation upon interaction with the parasite. Among them, were detected bands of 140, 120, 112, 94, 73, 67, and 56 kDa that match the molecular weights of proteins described as being tyrosine phosphorylated during events that lead to actin assembly in mononuclear phagocytes. The pretreatment of IC-21 macrophages with the tyrosine kinase inhibitor tyrphostin 23 inhibited trypomastigote uptake showing that tyrosine phosphorylation is important for the parasite penetration in this particular cell line. Immunofluorescence microscopy, using antibodies against p85, the regulatory subunit of phosphatidylinositol 3-kinase (PI 3-kinase), placed this enzyme also in the same sites, in accordance to what is reported for phagocytosis. We suggest that once the components of T. cruzi trypomastigotes surface are recognized by macrophage receptors, they trigger the activation of a tyrosine phosphorylation cascade, PI 3-kinase recruitment, and assembly of actin filaments at the site of initial cell-to-cell contact, resembling the events described during phagocytosis. These achievements support the model for a phagocytic-like actin-dependent invasion mechanism for T. cruzi trypomastigotes into macrophages.
authors.affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, RJ, Brazil.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Vieira
authors.forename: Juliana M F
authors.initials: JM
authors.lastname: Dutra
authors.forename: Tecia M U
authors.initials: TM
authors.lastname: Carvalho
authors.forename: Narcisa L
authors.initials: NL
authors.lastname: Cunha-e-Silva
authors.forename: Thaïs
authors.initials: T
authors.lastname: Souto-Padrón
authors.forename: Wanderley
authors.initials: W
authors.lastname: Souza
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000469:parasitology*; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000469:parasitology; D051379:Mice; D018613:Microscopy, Confocal; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction; D014349:Trypanosoma cruzi / Q000254:growth & development*; D014443:Tyrosine / Q000378:metabolism; D020032:Tyrphostins / Q000494:pharmacology
chemical_list: D000199:Actins; D004791:Enzyme Inhibitors; D020032:Tyrphostins; D014443:Tyrosine; D019869:Phosphatidylinositol 3-Kinases; D011505:Protein-Tyrosine Kinases; C060641:tyrphostin A23