Effects of alginate hydrogels and TGF-beta 1 on human dental pulp repair in vitro.
The objective of this study was to investigate the use of alginate hydrogels to present either exogenous or endogenous transforming growth factor (TGF)-beta 1 to the dentin-pulp complex to signal reparative processes. Hydrogels were prepared, applied to cultured human tooth slices and the effects on tertiary dentinogenesis examined histologically. Both TGF-beta 1-containing and acid-treated alginate hydrogels, but not untreated hydrogels, upregulated dentin matrix secretion and induced odontoblast-like cell differentiation with subsequent secretion of regular tubular dentin matrix on cut pulpal surfaces. It is concluded that TGF-beta 1 can signal both induction of odontoblast-like cell differentiation and upregulation of their matrix secretion in the human dentin-pulp complex. Alginate hydrogels provide an appropriate matrix in which dental regeneration can take place and may also be useful for delivery of growth factors, including TGF-beta s, to enhance the natural regenerative capacity of the dental pulp.
Dobie
K
K
School of Dentistry, University of Birmingham, St Chad's Queensway, Birmingham B4 6NN, UK.
Smith
G
G
Sloan
A J
AJ
Smith
A J
AJ
D000143:Acids / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D000464:Alginates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D003782:Dental Pulp / Q000503:physiopathology*; D003804:Dentin / Q000378:metabolism; D003810:Dentinogenesis / Q000187:drug effects*; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D020100:Hydrogels / Q000494:pharmacology*; D009804:Odontoblasts / Q000473:pathology; D009924:Organ Culture Techniques; D012038:Regeneration / Q000187:drug effects*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1; D015854:Up-Regulation
D000143:Acids; D000464:Alginates; D020100:Hydrogels; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1