[Screening of novel biomarkers for the detection of intraperitoneally disseminated cancer cells using human cDNA microarray].
In this study, we performed a global analysis of the differential gene expression of a gastric cancer cell line established from a primary main tumor (SNU-1) and that of other cell lines established from the metastasis to the peritoneal cavity (KATO-III, SNU-5, SNU-719, MKN45P, HS39). The application of a high-density cDNA microarray method made it possible to analyze the expression of approximately 21,168 genes. Our examinations of KATO-III, SNU-5, SNU-719, MKN45P, and HS39 showed that several genes were up-regulated in addition to expression of sequence tags (ESTs). The analysis revealed altered up-regulation of CD44 (cell adhesion), CEA, 14-3-3, Ubiquitin A and several kinds of ESTs in gastric cancer cells from malignant ascites. We then analyzed these gastric cancer cell lines with Northern blots and observed preferential up-regulation of these selected genes in cells prone to peritoneal dissemination. RT-PCR confirmed that several genes selected by DNA microarray were also overexpressed in clinical samples of malignant ascites. It is therefore considered that these genes may be related to the peritoneal dissemination of gastric cancers. The results of this global gene expression analysis of gastric cancer cells with peritoneal dissemination promise to provide new insights into the study of human gastric cancer peritoneal dissemination.
Sakakura
Chouhei
C
Dept. of Gastroenterological Surgery, Kyoto Prefectural University of Medicine.
Simomura
Katsumi
K
Shuichi
Kin
K
Nakase
Yuen
Y
Fukuda
Ken-ichiro
K
Takagi
Tsuyoshi
T
Hagiwara
Akeo
A
Ichikawa
Yasushi
Y
Nishizuka
Itaru
I
Ishikawa
Takashi
T
Okazaki
Yasushi
Y
Yamagishi
Hisakazu
H
D015415:Biomarkers / Q000032:analysis*; D015152:Blotting, Northern; D002460:Cell Line; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010534:Peritoneal Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000556:secondary*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D015415:Biomarkers