Sequence analysis and genomic organization of Aphid lethal paralysis virus: a new member of the family Dicistroviridae.
The complete nucleotide sequence of the genomic RNA of an aphid-infecting virus, Aphid lethal paralysis virus (ALPV), has been determined. The genome is 9812 nt in length and contains two long open reading frames (ORFs), which are separated by an intergenic region of 163 nt. The first ORF (5' ORF) is preceded by an untranslated leader sequence of 506 nt, while an untranslated region of 571 nt follows the second ORF (3' ORF). The deduced amino acid sequences of the 5' ORF and 3' ORF products respectively showed similarity to the non-structural and structural proteins of members of the newly recognized genus Cripavirus (family Dicistroviridae). On the basis of the observed sequence similarities and identical genome organization, it is proposed that ALPV belongs to this genus. Phylogenetic analysis showed that ALPV is most closely related to Rhopalosiphum padi virus, and groups in a cluster with Drosophila C virus and Cricket paralysis virus, while the other members of this genus are more distantly related. Infectivity experiments showed that ALPV can not only infect aphid species but is also able to infect the whitefly Trialeurodes vaporariorum, extending its host range to another family of the order Hemiptera.
van Munster
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Plant Research International, PO Box 16, 6700 AA Wageningen, The Netherlands1.
Dullemans
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AM
Plant Research International, PO Box 16, 6700 AA Wageningen, The Netherlands1.
Verbeek
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M
Plant Research International, PO Box 16, 6700 AA Wageningen, The Netherlands1.
van den Heuvel
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JFJM
De Ruiter Seeds, PO Box 1050, 2660 BB Bergschenhoek, The Netherlands2.
Clérivet
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Unité de Phytopathologie, IRD, BP 5045 Montpellier, France3.
van der Wilk
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Plant Research International, PO Box 16, 6700 AA Wageningen, The Netherlands1.
D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000472:pathogenicity / Q000821:virology*; D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D016679:Genome, Viral; D006430:Hemiptera / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012328:RNA Viruses / Q000737:chemistry / Q000145:classification* / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins / Q000235:genetics
D036022:Capsid Proteins; D017361:Viral Nonstructural Proteins