title: A natural recessive resistance gene against potato virus Y in pepper corresponds to the eukaryotic initiation factor 4E (eIF4E).
abstract: We show here that the pvr2 locus in pepper, conferring recessive resistance against strains of potato virus Y (PVY), corresponds to a eukaryotic initiation factor 4E (eIF4E) gene. RFLP analysis on the PVY-susceptible and resistant pepper cultivars, using an eIF4E cDNA from tobacco as probe, revealed perfect map co-segregation between a polymorphism in the eIF4E gene and the pvr2 alleles, pvr2(1) (resistant to PVY-0) and pvr2(2) (resistant to PVY-0 and 1). The cloned pepper eIF4E cDNA encoded a 228 amino acid polypeptide with 70-86% nucleotide sequence identity with other plant eIF4Es. The sequences of eIF4E protein from two PVY-susceptible cultivars were identical and differed from the eIF4E sequences of the two PVY-resistant cultivars Yolo Y (YY) (pvr2(1)) and FloridaVR2 (F) (pvr2(2)) at two amino acids, a mutation common to both resistant genotypes and a second mutation specific to each. Complementation experiments were used to show that the eIF4E gene corresponds to pvr2. Thus, potato virus X-mediated transient expression of eIF4E from susceptible cultivar Yolo Wonder (YW) in the resistant genotype YY resulted in loss of resistance to subsequent PVY-0 inoculation and transient expression of eIF4E from YY (resistant to PVY-0; susceptible to PVY-1) rendered genotype F susceptible to PVY-1. Several lines of evidence indicate that interaction between the potyvirus genome-linked protein (VPg) and eIF4E are important for virus infectivity, suggesting that the recessive resistance could be due to incompatibility between the VPg and eIF4E in the resistant genotype.
authors.affiliation: Genetics and Breeding of Fruits and Vegetable, Dom St Maurice, BP94, F-84143 Montfavet.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Ruffel
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Dussault
authors.forename: Alain
authors.initials: A
authors.lastname: Palloix
authors.forename: Benoît
authors.initials: B
authors.lastname: Moury
authors.forename: Abdelhafid
authors.initials: A
authors.lastname: Bendahmane
authors.forename: Christophe
authors.initials: C
authors.lastname: Robaglia
authors.forename: Carole
authors.initials: C
authors.lastname: Caranta
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D002212:Capsicum / Q000235:genetics* / Q000821:virology; D003001:Cloning, Molecular; D039561:Eukaryotic Initiation Factor-4E / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D015967:Gene Expression Regulation, Viral; D005808:Genes, Recessive / Q000235:genetics; D005816:Genetic Complementation Test; D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D017800:Potyvirus / Q000235:genetics / Q000254:growth & development*; D014758:Viral Core Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D039561:Eukaryotic Initiation Factor-4E; D014758:Viral Core Proteins