Effect of Tween 80 and monensin on ruminal fermentation of the diet containing 70% wheat straw treated by white-rot fungus in artificial rumen.
The objective of this study was to investigate the effect of Tween 80 and monensin on rumen fermentation of the diet containing 70% wheat straw treated by white-rot fungus Pleurotus tuber-regium (TWS-PT) and 30% barley in artificial rumen (RUSITEC). The RUSITEC consisted of four fermentation vessels (V1, V2, V3, V4): V1 was without additives (control), V2 received daily 10 mg of monensin, V3 received daily 0.5% Tween 80 (vol.wt-1) and V4 involved the combination of 10 mg of monensin with 0.5% Tween 80 (vol.wt-1). After an adaptation period (6 days) the fermentation parameters were determined for six consecutive days. Tween 80 did not affect the rumen fermentation of the diet consisting 70% TWS-PT and 30% barley in RUSITEC. Monensin affected the rumen fermentation of the diet by the decreased degradability of DM, neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose, cellulose (p < 0.001), the decrease of methane production (p < 0.001) and the higher proportion of propionate within the volatile fatty acids (p < 0.001) in comparison to control. Tween 80 did not improve the potency of monensin. Only some indices of the increase mol% of propionate (about 3.4%) and the decrease of methane production (about 0.47 mmol.day-1) were found by using Tween 80 plus monensin in comparison to use of monensin alone.
Jalc
Dusan
D
Institute of Animal Physiology Slovak Academy of Sciences, Kosice, Slovak Republic.
D000821:Animal Feed; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D005285:Fermentation / Q000187:drug effects; D066298:In Vitro Techniques; D007476:Ionophores / Q000494:pharmacology; D008985:Monensin / Q000494:pharmacology*; D020076:Pleurotus / Q000378:metabolism; D011136:Polysorbates / Q000494:pharmacology*; D012417:Rumen / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D013501:Surface-Active Agents / Q000494:pharmacology*; D014908:Triticum / Q000378:metabolism / Q000382:microbiology*
D000935:Antifungal Agents; D007476:Ionophores; D011136:Polysorbates; D013501:Surface-Active Agents; D008985:Monensin