Diminution of experimental autoimmune uveoretinitis (EAU) in mice depleted of NK cells.
To evaluate the potential role of NK1.1 (CD161c) cells in autoimmune uveoretinitis, we treated experimental autoimmune uveoretinitis (EAU)-susceptible mice with anti-CD161c antibodies (PK136) to deplete natural killer (NK) cells. Injection of anti-CD161c antibodies deleted NK cells from the peripheral blood of EAU-susceptible mice. The T cell proliferative response against the ocular autoantigen K2 was not suppressed in mice treated with anti-CD161c antibody when compared with T cells from control mice. Although mice treated with anti-CD161c developed EAU, the clinical severity on days 17 and 19 after induction of EAU was significantly mild in anti-CD161c-treated mice compared with control mice. In addition, the histopathological severity of EAU was significantly milder in mice treated with anti-CD161c antibodies than controls 21 days after induction of EAU. Our results indicate that the severity of EAU is augmented by NK1.1(+) NK cells.
Kitaichi
Nobuyoshi
N
Schepens Eye Research Institute and the Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02114, USA.
Kotake
Satoshi
S
Morohashi
Taiki
T
Onoé
Kazunori
K
Ohno
Shigeaki
S
Taylor
Andrew W
AW
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000494:pharmacology; D000950:Antigens, Ly; D000954:Antigens, Surface / Q000276:immunology; D001327:Autoimmune Diseases / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D015551:Autoimmunity; D005260:Female; D007694:Killer Cells, Natural / Q000502:physiology*; D037181:Lectins, C-Type / Q000276:immunology; D008212:Lymphocyte Depletion; D051379:Mice; D008815:Mice, Inbred Strains; D055651:NK Cell Lectin-Like Receptor Subfamily B; D012173:Retinitis / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D014605:Uveitis / Q000209:etiology* / Q000276:immunology / Q000473:pathology
D000906:Antibodies; D000950:Antigens, Ly; D000954:Antigens, Surface; C527211:Klrb1c protein, mouse; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B