title: Circadian clockwork genes are expressed in the reproductive tract and conceptus of the early pregnant mouse.
abstract: Circadian genes are expressed in some peripheral tissues, but the expression status of the female reproductive tract and the conceptus over the preimplantation period is unknown. Oocytes, uterine, oviducal tissues and preimplantation conceptuses from days 1-4 of mouse pregnancy were analysed for transcript presence by reverse transcription polymerase chain reaction. Transcripts encoded by the seven known mammalian canonical circadian genes (Per1-3, Cry1-2, Bmal1 and Clock), plus the mammalian genetic homologue of the Drosophila canonical gene Timeless, were detected in the uteri and oviducts taken from mice on days 1-4 of pregnancy and in unfertilized oocytes. After fertilization, transcripts for Per1, Cry1, Bmal1, Clock and Tim have been detected unambiguously. Transcript levels for each of these five genes fall at the two-cell stage, but are restored rapidly for Per1, Cry1 and Bmal1, presumptively by zygotic gene expression. In contrast, transcripts for Clock and Tim recover more slowly. It is concluded that circadian genes are expressed, and may therefore have a role, during the early development of the mammal.
authors.affiliation: Department of Physiology (F13), University of Sydney, NSW 2006, Australia. mhj@mole.bio.cam.ac.uk
authors.forename: Martin H
authors.initials: MH
authors.lastname: Johnson
authors.forename: Alice
authors.initials: A
authors.lastname: Lim
authors.forename: Dinukshi
authors.initials: D
authors.lastname: Fernando
authors.forename: Margot L
authors.initials: ML
authors.lastname: Day
mesh_terms: D000818:Animals; D002940:Circadian Rhythm / Q000235:genetics*; D005187:Fallopian Tubes / Q000737:chemistry*; D005260:Female; D005333:Fetus / Q000378:metabolism*; D015870:Gene Expression; D005865:Gestational Age; D051379:Mice; D009865:Oocytes / Q000737:chemistry; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014599:Uterus / Q000737:chemistry*