title: [Optimizing histological image data for 3-D reconstruction using an image equalizer].
abstract: Bone cells form a wired network within the extracellular bone matrix. To analyse this complex 3D structure, we employed a confocal fluorescence imaging procedure to visualize live bone cells within their native surrounding. By means of newly developed image processing software, the "Image-Equalizer", we aimed to enhanced the contrast and eliminize artefacts in such a way that cell bodies as well as fine interconnecting processes were visible.
authors.affiliation: Bereich Med. Informatik, Fachhochschule Dortmund. alexander.roth@fh-dortmund.de
authors.forename: A
authors.initials: A
authors.lastname: Roth
authors.forename: K
authors.initials: K
authors.lastname: Melzer
authors.forename: K
authors.initials: K
authors.lastname: Annacker
authors.forename: H G
authors.initials: HG
authors.lastname: Lipinski
authors.forename: M
authors.initials: M
authors.lastname: Wiemann
authors.forename: D
authors.initials: D
authors.lastname: Bingmann
mesh_terms: D000465:Algorithms; D000818:Animals; D016477:Artifacts; D001842:Bone and Bones / Q000166:cytology*; D002478:Cells, Cultured; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D010781:Photography / Q000295:instrumentation*; D051381:Rats