IL-18 production in human pulmonary and pleural tuberculosis.
Interleukin-18 (IL-18) has multiple important pro-inflammatory effects, including the induction of interferon-gamma (IFN-gamma) in various diseases. In this study, we investigated the IL-18-producing activities in human pulmonary and pleural tuberculosis (TB) in response to purified protein derivative (PPD) antigen (Ag) from Mycobacterium tuberculosis. The most significant IL-18 production was found in chronic refractory TB (CRTB) patients. However, IFN-gamma production in CRTB patients was significantly less than that in healthy tuberculin reactors or in patients with tuberculous pleurisy (TBP). Elevated levels of both IL-18 and IFN-gamma were found in pleural fluids from TBP patients. In vitro production of IL-18 was dramatically decreased following an 18 h stimulation with PPD. However, IFN-gamma was markedly increased in pleural mononuclear cells from TBP patients after in vitro stimulation with PPD. The mesothelial cell type was the main source of pro-IL-18 in pleural cells from TBP patients, suggesting an important role for these cells in TBP. Taken together, these data indicate that IL-18 is elevated in peripheral blood mononuclear cells from CRTB patients, as well as at the site of TBP, indicating a possible role for IL-18 in both protective immunity and pathologic responses in human TB.
Song
C-H
CH
Department of Microbiology, College of Medicine, Chungnam National University, Taejon, Korea.
Lee
J-S
JS
Nam
H-H
HH
Kim
J-M
JM
Suhr
J-W
JW
Jung
S-S
SS
Na
M-J
MJ
Paik
T-H
TH
Kim
H-J
HJ
Park
J-K
JK
Jo
E-K
EK
D002478:Cells, Cultured; D004848:Epithelium / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D020382:Interleukin-18 / Q000096:biosynthesis* / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000276:immunology; D010996:Pleural Effusion / Q000276:immunology; D010998:Pleurisy / Q000276:immunology; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D014373:Tuberculin / Q000276:immunology; D014396:Tuberculosis, Pleural / Q000276:immunology* / Q000473:pathology; D014397:Tuberculosis, Pulmonary / Q000276:immunology*; D015854:Up-Regulation
D020382:Interleukin-18; D011498:Protein Precursors; D012333:RNA, Messenger; D014373:Tuberculin; D007371:Interferon-gamma