title: [The effects of antisenes oligodeoxynucleotide on the cyclic nucleotide monophosphates in smooth muscle cells of human corpus cavernosum].
abstract: OBJECTIVE
  To investigate the effects of antisense oligodeoxynucleotide(ASON) on the cyclic nucleotide monophosphates (cNMP) in smooth muscle cells of human corpus cavernosum, and provide experimental groundwork for the gene therapy of erectile dysfunction.
  
  
  METHODS
  PDE5 gene ASON(containing exon 1) was transfected into the corpus cavernosum smooth muscle cells with the presence of liposome DOTAP. Another sense oligodeoxynucleotide(SON) and 1% of bovine serum were also transducted into the cells as controls. Two of cNMP, cAMP and cGMP, were probed and measured by ELISA at 1, 2, 4, 6, 10, 24 and 48 h after transfection.
  
  
  RESULTS
  After transfection, the level of cGMP(1-6 h) in human corpus cavernosum smooth muscle cells was significantly higher than that in controls(P < 0.01).
  
  
  CONCLUSIONS
  The PDE5 gene ASON had been showed to manifest stimulative effect on the cGMP in smooth muscle cells of human corpus cavernosum in vitro, and it provides experimental groundwork for the gene therapy of erectile dysfunction.
authors.affiliation: Department of Urology, People's Hospital, Peking University, Beijing 100044, China. baiwenjunbaiyue@sohu.com
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Bai
authors.forename: Shu-Kun
authors.initials: SK
authors.lastname: Hou
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Yan
authors.forename: Pei-Ying
authors.initials: PY
authors.lastname: He
authors.forename: Qing-Ping
authors.initials: QP
authors.lastname: Deng
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Hu
authors.forename: Kao-Peng
authors.initials: KP
authors.lastname: Guan
mesh_terms: D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000037:antagonists & inhibitors* / Q000235:genetics; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; D006801:Humans; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects* / Q000378:metabolism; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D010413:Penis / Q000166:cytology*
chemical_list: D020319:Oligodeoxyribonucleotides, Antisense; D000242:Cyclic AMP; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; C517381:PDE5A protein, human; D006152:Cyclic GMP