Monoclonal antibody against free beta-subunit of human chorionic gonadotropin.
Spleen cells from BALB/c mice immunized with free native human chorionic gonadotropin hormone beta-subunit (beta hCG) were fused with mouse myeloma cells (P3/X63-Ag8) and one hybridoma secreting monoclonal antibodies (MAbs), was obtained. This hybridoma specifically recognizes beta hCG and does not cross-react with other human glycoprotein hormones, such as luteinizing hormone (LH), follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), and human chorionic gonadotropin (hCG). The MAb was of the IgG(1) subclass and ascitic fluid from this hybridoma was purified by affinity chromatography on Protein A-Sepharose CL-4B column to isolate the IgG(1) active fraction. The affinity constant of this MAb was 1.5 x 10(10)M(-1).
Baluja Conde
Ileana B
IB
Laboratorio de Anticuerpos Monoclonales, Centro de Inmunoensayo, Apartado 6653, Ave 25 y 134, Cubanacán, Playa, Ciudad Habana, Cuba. iqmonoclol@cie.sld.cu
Brito Moreno
Adys I
AI
Acosta Bas
Carmen
C
Zalueta Rodríguez
Orlando
O
Melchor Rodríguez
Antonio
A
Amores Sanchéz
Isis
I
Martínez
Luisa
L
Hernández
Milenen
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D006063:Chorionic Gonadotropin / Q000737:chemistry* / Q000276:immunology*; D002847:Chromatography, Agarose; D004305:Dose-Response Relationship, Drug; D004314:Down Syndrome / Q000175:diagnosis; D000939:Epitopes; D005640:Follicle Stimulating Hormone / Q000737:chemistry; D006801:Humans; D006825:Hybridomas; D007074:Immunoglobulin G / Q000378:metabolism; D007700:Kinetics; D007986:Luteinizing Hormone / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013972:Thyrotropin / Q000737:chemistry
D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D000939:Epitopes; D007074:Immunoglobulin G; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin