Single-molecule fluorescence of nucleic acids.
Less than a decade old, single-molecule fluorescence of nucleic acids has rapidly become an important tool in the arsenal of biological probes. A variety of novel approaches to investigate conformational dynamics, catalytic mechanisms, folding pathways and protein-nucleic-acid interactions have recently been devised for nucleic acids using this technique. Combined with biomechanical tools and ensemble measurements, single-molecule fluorescence methods extend our ability to observe and understand biomolecules and complex biological processes.
Mollova
Emilia T
ET
Department of Biochemistry, Stanford University, Stanford, CA 94305, USA. mollova@cmgm.stanford.edu
D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004735:Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005456:Fluorescent Dyes; D009696:Nucleic Acids; D012313:RNA; D004247:DNA