Neurogenin3 participates in gliogenesis in the developing vertebrate spinal cord.
To study the role of basic helix-loop-helix (bHLH) transcription factors in gliogenesis, we examined whether bHLH transcription factors were expressed in glial precursor cells and participated in regulating oligodendrocyte and astrocyte development. As assessed by reverse transcription-polymerase chain reaction (RT-PCR), Neurogenin3 (Ngn3) was transiently expressed in bipotential glial cells fated to become either oligodendrocytes or astrocytes. Mice lacking Ngn3 displayed a loss of Nkx2.2 expression, a transcription factor required for proper oligodendrogliogenesis. Furthermore, a reduction in the expression of myelin basic protein (MBP), proteolipid protein (PLP), and glial fibrillary acidic protein (GFAP), markers for mature oligodendrocytes and astrocytes, was observed in the Ngn3 null mice. Overexpression of Ngn3 was sufficient to drive expression from the PLP promoter in transient cotransfection assays. Overall, the data suggest that Ngn3 may regulate glial differentiation at a developmental stage prior to the segregation of the oligodendrocyte and astrocyte lineage.
Lee
Jeffrey
J
IGBMC, Universite Louis Pasteur, 1 rue Laurent Fries, BP10142, 67404 Illkirch Cedex, CU de Strasbourg, France.
Wu
Yuanyuan
Y
Qi
Yingchuan
Y
Xue
Haipeng
H
Liu
Ying
Y
Scheel
David
D
German
Michael
M
Qiu
Mengsheng
M
Guillemot
Francois
F
Rao
Mahendra
M
Gradwohl
Gerard
G
D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D017931:DNA Primers; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins / Q000235:genetics; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020287:In Situ Nick-End Labeling; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D009457:Neuroglia / Q000201:enzymology*; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013116:Spinal Cord / Q000196:embryology*; D014157:Transcription Factors / Q000235:genetics; D029961:Zebrafish Proteins
D051792:Basic Helix-Loop-Helix Proteins; D017931:DNA Primers; D005904:Glial Fibrillary Acidic Protein; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D029961:Zebrafish Proteins; C498696:Neurog3 protein, rat; C000715627:Nkx2-2 protein, mouse; C000715628:Nkx2-2 protein, rat; C000715630:nkx2.2b protein, zebrafish