Loss of G protein gamma 7 alters behavior and reduces striatal alpha(olf) level and cAMP production.
The G protein beta gamma-dimer is required for receptor interaction and effector regulation. However, previous approaches have not identified the physiologic roles of individual subtypes in these processes. We used a gene knockout approach to demonstrate a unique role for the G protein gamma(7)-subunit in mice. Notably, deletion of Gng7 caused behavioral changes that were associated with reductions in the alpha(olf)-subunit content and adenylyl cyclase activity of the striatum. These data demonstrate that an individual gamma-subunit contributes to the specificity of a given signaling pathway and controls the formation or stability of a particular G protein heterotrimer.
Schwindinger
William F
WF
Weis Center for Research, Geisinger Clinic, Danville, Pennsylvania 17822, USA.
Betz
Kelly S
KS
Giger
Kathryn E
KE
Sabol
Angela
A
Bronson
Sarah K
SK
Robishaw
Janet D
JD
D000818:Animals; D001483:Base Sequence; D003342:Corpus Striatum / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D017931:DNA Primers; D019281:Dimerization; D005260:Female; D044385:GTP-Binding Protein alpha Subunits; D020962:Heterotrimeric GTP-Binding Proteins / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D044385:GTP-Binding Protein alpha Subunits; C084618:olfactory G protein subunit alpha olf; D000242:Cyclic AMP; D020962:Heterotrimeric GTP-Binding Proteins