title: Infection of myocytes with chlamydiae.
abstract: Chlamydial infection has been associated with myocarditis in animals and humans. However, the mechanism resulting in myocarditis following infection is not known. Here, evidence is presented that both Chlamydia trachomatis and Chlamydia pneumoniae can infect and replicate in myocytes isolated from neonate rats. The infected myocytes contained chlamydial inclusions, indicative of chlamydial growth, and infectious particles were recovered from the infected myocytes. It was also found that chlamydial infection at a late stage induced significant damage to the infected myocytes, as evidenced by an increased lactate dehydrogenase release, reactive oxygen species production and a reduced ATP level. However, no nuclear apoptosis was detected in the infected myocytes. Collectively, these observations have demonstrated that Chlamydia spp. are able to both infect and damage myocytes, suggesting a potential role of chlamydial infection in myocarditis.
authors.affiliation: Faculty of Pharmacy, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada1.
authors.forename: Guqi
authors.initials: G
authors.lastname: Wang
authors.affiliation: Faculty of Pharmacy, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada1.
authors.forename: Frank
authors.initials: F
authors.lastname: Burczynski
authors.affiliation: Faculty of Pharmacy, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada1.
authors.forename: Brian
authors.initials: B
authors.lastname: Hasinoff
authors.affiliation: Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA2.
authors.forename: Guangming
authors.initials: G
authors.lastname: Zhong
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002690:Chlamydia Infections / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000472:pathogenicity*; D016993:Chlamydophila pneumoniae / Q000472:pathogenicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000382:microbiology*; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase