[Effect of human calcyclin binding protein encoding gene on development of multiple drug resistance in gastric cancer].
OBJECTIVE
To study the effect of human calcyclin binding protein (CacyBP) encoding gene on the development of multiple drug resistance in gastric cancer.


METHODS
hCacyBP sense nucleic acid eukaryotic expression vector (pcDNA3.1/hCacyBP +) was constructed and then transfected steadily into the gastric cancer drug sensitive cell (SGC7901) mediated by lipofectamine ( trade mark ) 2000. RT-PCR was used to measure the CacyBP mRNA expression level. MTT was used to measure the adriamycin (ADR) drug sensitivity of SGC7901 and SGC7901 after transfection. FCM was used to measure the average ADR accumulation concentration and cell cycle of SGC7901 and SGC7901 after transfection.


RESULTS
The hCacyBP mRNA expression level of SGC7901 transfected with pcDNA3.1/hCacyBP + was higher than SGC7901 transfected with pcDNA3.1 or SGC7901, with the higher survival rate in the former. The average ADR accumulation concentration in SGC7901 and SGC7901 transfected with pcDNA3.1 or pcDNA3.1/hCacyBP + was 5.64, 5.49 and 5.17, respectively. The G(1) phase cell proportion of SGC7901 transfected with pcDNA3.1/hCacyBP + or pcDNA3.1 was reduced slightly but G(2) and S phases increased slightly as compared with SGC7901.


CONCLUSIONS
Calcyclin binding protein may play a certain role in gastric cancer drug resistance.
Hu
Wenhua
W
Department of Gastroenterology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Yin
Fang
F
Jin
Xiaohang
X
Fan
Daiming
D
D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000502:physiology*; D018076:DNA, Complementary / Q000032:analysis; D018432:Drug Resistance, Multiple / Q000502:physiology*; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D004354:Drug Screening Assays, Antitumor; D018014:Gene Transfer Techniques; D006801:Humans; D008969:Molecular Sequence Data; D037121:Plant Lectins; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
C491778:CACYBP protein, human; D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D037121:Plant Lectins