title: Methylphosphonate LNA: a locked nucleic acid with a methylphosphonate linkage.
abstract: Synthesis of an oligonucleotide containing one methylphosphonate locked nucleic acid (LNA) thymine monomer using the phosphoramidite approach is described. The binding affinity of this 9-mer methylphosphonate LNA towards complementary DNA and RNA oligonucleotides was increased compared to the reference DNA, but decreased compared to the reference LNA. In the 9-mer sequence context studied, introduction of a single methylphosphonate LNA monomer, contrary to a single LNA monomer, efficiently inhibits 3'-exonucleolytic degradation.
authors.affiliation: Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark.
authors.forename: Anne
authors.initials: A
authors.lastname: Lauritsen
authors.forename: Britta M
authors.initials: BM
authors.lastname: Dahl
authors.forename: Otto
authors.initials: O
authors.lastname: Dahl
authors.forename: Birte
authors.initials: B
authors.lastname: Vester
authors.forename: Jesper
authors.initials: J
authors.lastname: Wengel
mesh_terms: D004247:DNA / Q000138:chemical synthesis; D004277:DNA, Single-Stranded / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000494:pharmacology; D005092:Exonucleases / Q000037:antagonists & inhibitors; D007202:Indicators and Reagents; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000737:chemistry*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D012313:RNA / Q000138:chemical synthesis
chemical_list: D004277:DNA, Single-Stranded; D004791:Enzyme Inhibitors; D007202:Indicators and Reagents; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C032627:methylphosphonic acid; D012313:RNA; D004247:DNA; D005092:Exonucleases; D010727:Phosphoric Diester Hydrolases