title: [Effect of inhibitor RNA on intracellular inhibition of viral gene expression in 5'-noncoding region of hepatitis C virus].
abstract: OBJECTIVE
  To determine the effect of internal ribosome entry site (IRES)specific inhibitor RNA (IRNA) on viral gene expression mediated by HCV 5' noncoding region (NCR) in human hepatic carcinoma cell line.
  
  
  METHODS
  Cotransfection of pCMVNCRluc containing 5'NCR-luc fusion genes and eukaryotic vectors of IRNA into human hepatic carcinoma cells (HHCC) was performed and the luciferase activity of reporter gene was measured by luminometer.
  
  
  RESULTS
  pCMVNCRluc was efficiently expressed in HHCC. IRES specific IRNA significantly inhibited the expression of luc mediated by HCV IRES by 50% to 90%, but the mutant IRNA lost the activity of inhibition.
  
  
  CONCLUSIONS
  IRNA can inhibit HCV IRES mediated gene expression in vivo.
authors.affiliation: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. ghx69514@163.com
authors.forename: Xuesong
authors.initials: X
authors.lastname: Liang
authors.forename: Yongxing
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jianqi
authors.initials: J
authors.lastname: Lian
authors.forename: Qinghe
authors.initials: Q
authors.lastname: Nie
authors.forename: Zhansheng
authors.initials: Z
authors.lastname: Jia
mesh_terms: D020121:5' Untranslated Regions / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects*; D005814:Genes, Viral; D015316:Genetic Therapy; D016174:Hepacivirus / Q000187:drug effects / Q000235:genetics*; D006526:Hepatitis C / Q000628:therapy; D006801:Humans; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D020121:5' Untranslated Regions; D012330:RNA, Double-Stranded