[Calcium 2+ Response of Lymphocytes to Standard Test-Substances in Patients With Ischemic Heart Disease and Heart Failure]
Changes of [Ca 2+]i were studied in peripheral blood lymphocytes of patients with ischemic heart disease and heart failure. Basal [Ca 2+]i and its changes under action of mitogenes (PHA, Con A; 10 mcg/ml), butylhydroxyquinone (BHQ; 9-14 mcM) and arachidonic acid (3-10 mc/M) were measured. Patients with heart failure had pronounced changes of kinetics and amplitude of Ca 2+ response of lymphocytes to mitogenes, significantly higher amplitudes of responses to BHQ (in patients with stage IIB and III heart failure), as well as additional increment of [Ca 2+]i in response to rachidonic acid during action of optimal concentrations of BHQ. This data allowed to propose presence of supplementary inositol triphosphate insensitive calcium depots in cells of patients with heart failure. Furthermore it was possible to assume lowering of sensitivity of Ca 2+ ATP-ase of plasmatic membranes to its natural regulators.
Astashkin
E. I.
EI
Moscow Medical Academy im I.M. Sechenova, Bolshaia Pirogovskaia, 2/6, 119881 Moscow, Russia.
Glezer
M. G.
MG
Khodorova
A. B.
AB
Arabidze
Gr. G.
GG
Orlov
L. L.
LL
Poliakova
O. B.
OB