Inhibition of LPS-induced NO production by the oleogum resin of Commiphora wightii and its constituents.
Three new (1-3) and five known compounds (4-8) were isolated from the oleogum resin of Commiphora wightii (Arnott.) Bhanol. Their structures were elucidated by spectroscopic and chemical methods. The MeOH extract and the EtOAc-sol. fraction were found to demonstrate significant inhibition of NO formation in lipopolysaccharide (LPS)-activated murine macrophages J774.1 in vitro (IC(50) values of 16.4 and 12.8 microg/ml, respectively). When compared with curcumin (IC(50) value of 12.3 microM), Z- and E-Guggulsterones (4 and 5, respectively) were the most potent inhibitors of NO production (IC(50) values of 1.1 and 3.3 microM, respectively), followed by myrrhanol A (7) and myrrhanone A (8) (IC(50) values of 21.1 and 42.3 microM, respectively). Guggulsterone-M (1) and its didehydro derivative (2) were weak inhibitors, while guggulsterols I (6) and Y (3) were inactive (IC(50) >500 microM).
Meselhy
Meselhy R
MR
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr el-Aini Street, 11562 Cairo, Egypt. meselhyragab@yahoo.com
D000818:Animals; D002478:Cells, Cultured; D029744:Commiphora / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism*; D012116:Resins, Plant / Q000737:chemistry* / Q000494:pharmacology*
D008070:Lipopolysaccharides; D012116:Resins, Plant; D009569:Nitric Oxide