Inhibition of Trypanosoma cruzi growth by medical plant extracts.
This study describes the screening of extracts obtained from 18 plants and two fungi used in the Chinese and Mediterranean traditional medicines on epimastigote forms of Trypanosoma cruzi. The extracts were tested against epimastigote of T. cruzi Bra C15C2 clone in vitro at 27 degrees C and at a concentration of 250 microg/ml in axenic culture. Angelica dahurica, A. pubescens, A. sinensis, Astragalus membranaceus, Coptis chinensis, Haplophyllum hispanicum, Phellodendron amurense, Poria cocos, Ranunculus sceleratus and Scutellaria baicalensis showed significant effects against the parasite with a percentage of growth inhibition between 20 and 100%. C. chinensis and R. sceleratus showed the greatest activity with IC(50) values of 1.7 microg/ml for C. chinensis and 10.7 microg/ml for R. sceleratus. These activities are greater than that of allopurinol. C. chinesis and R. sceleratus extracts did not show cytotoxic effects on rat polimorphonuclear cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and lactic dehydrogenase assays. These results allowed us to suggest that R. sceleratus and C. chinensis could be a source of new compounds clinically active against T. cruzi.
Schinella
G R
GR
Cátedra de Farmacología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Calles 60 y 120, La Plata 1900, Argentina. schinell@lacasilla.com.ar
Tournier
H A
HA
Prieto
J M
JM
Ríos
J L
JL
Buschiazzo
H
H
Zaidenberg
A
A
D000818:Animals; D027885:Astragalus propinquus; D004305:Dose-Response Relationship, Drug; D005658:Fungi / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D007962:Leukocytes / Q000187:drug effects; D016015:Logistic Models; D008297:Male; D008516:Medicine, Chinese Traditional; D008519:Medicine, Traditional; D019083:Mediterranean Region; D010936:Plant Extracts / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000254:growth & development*
D010936:Plant Extracts