[Expression of nicotinamide edenine dinucleotide dehydrogenase gene in placenta of patients with pregnancy induced hypertension].
OBJECTIVE
To investigate the expression of nicotinamide edenine dinucleotide (NADH) dehydrogenase gene in the placenta of patients with pregnancy induced hypertension (PIH) and the role of NADH dehydrogenase in PIH pathogenesis.


METHODS
Using (32)P dATP labeled human NADH dehydrogenase cDNA probe, the expression of NADH dehydrogenase gene in placental tissues of 10 normal late pregnant (NLP) women and 10 PIH patients was detected by dot blot. Mean A of each hybridization dot was measured by image analysis system to compare NADH dehydrogenase mRNA level in PIH placentae with that in NLP placenta.


RESULTS
The NADH dehydrogenase gene values in patients with PIH and NLP were 21 +/- 6 and 56 +/- 16. The level of NADH dehydrogenase mRNA in PIH placenta was significantly higher than that in normal placenta (P < 0.05).


CONCLUSIONS
Elevated expression of NADH dehydrogenase gene may play a role in PIH pathogenesis.
Yang
Liyuan
L
Department of Obstetrics and Gynecology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Yao
Yuanqing
Y
Shi
Yongquan
Y
Wang
Xin
X
Shi
Juanzi
J
D000328:Adult; D017209:Apoptosis; D005260:Female; D006801:Humans; D006973:Hypertension / Q000201:enzymology*; D015227:Lipid Peroxidation; D009245:NADH Dehydrogenase / Q000235:genetics* / Q000502:physiology; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000201:enzymology*
D009245:NADH Dehydrogenase