title: Loss of Igf2 imprinting in monoclonal mouse hepatic tumor cells is not associated with abnormal methylation patterns for the H19, Igf2, and Kvlqt1 differentially methylated regions.
abstract: IGFII, the peptide encoded by the Igf2 gene, is a broad spectrum mitogen with important roles in prenatal growth as well as cancer progression. Igf2 is transcribed from the paternally inherited allele, whereas the linked H19 is transcribed from the maternal allele. Igf2 imprinting is thought to be maintained by differentially methylated regions (DMRs) located at multiple sites such as upstream of H19 and Igf2 and within Kvlqt1 loci. Biallelic expression (loss of imprinting (LOI)) of Igf2 is frequently observed in cancers, and a subset of Wilms' and intestinal tumors have been shown to exhibit abnormal methylation at H19DMR associated with loss of maternal H19 expression, but it is not known whether such changes are common in other neoplasms. Because cancers consist of diverse cell populations with and without Igf2 LOI, we established four independent monoclonal cell lines with Igf2 LOI from mouse hepatic tumors. We here demonstrate retention of normal differential methylation at H19, Igf2, or Kvlqt1 DMR by all of the cell lines. Furthermore, H19 was found to be expressed exclusively from the maternal allele, and levels of CTCF, a multifunctional nuclear factor that has an important role in the Igf2 imprinting, were comparable with those in normal hepatic tissues with no mutational changes detected. These data indicate that Igf2 LOI in tumor cells is not necessarily linked to abnormal methylation at H19, Igf2, or Kvlqt1 loci.
authors.affiliation: Department of Pathology, Asahikawa Medical College, 2-1-1-1 East, Midorigaoka, Asahikawa 078-8510, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Ishizaki
authors.forename: Masumi
authors.initials: M
authors.lastname: Yoshie
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yaginuma
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tanaka
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D001483:Base Sequence; D002273:Carcinogens; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000235:genetics*; D004052:Diethylnitrosamine; D018392:Genomic Imprinting; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D051656:KCNQ Potassium Channels; D051657:KCNQ1 Potassium Channel; D008113:Liver Neoplasms / Q000139:chemically induced / Q000235:genetics; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics*; D051379:Mice; D015221:Potassium Channels / Q000235:genetics*; D024642:Potassium Channels, Voltage-Gated; D062085:RNA, Long Noncoding; D012333:RNA, Messenger / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D002273:Carcinogens; D004273:DNA, Neoplasm; C094335:H19 long non-coding RNA; D051656:KCNQ Potassium Channels; D051657:KCNQ1 Potassium Channel; C496910:Kcnq1 protein, mouse; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D022661:RNA, Untranslated; D004052:Diethylnitrosamine; D007335:Insulin-Like Growth Factor II