title: Prediction of chemosensitivity for patients with acute myeloid leukemia, according to expression levels of 28 genes selected by genome-wide complementary DNA microarray analysis.
abstract: To identify genes involved in the sensitivity of acute myeloid leukemia (AML) cells to chemotherapy, we monitored gene-expression profiles of cancer cells from 76 AML patients using a cDNA microarray consisting of 23,040 genes. We identified 63 genes that were commonly overexpressed and 372 genes suppressed in AML. Because these genes represent key molecules for disclosing the molecular mechanisms of AML, they may be potential targets for drug development. We also found 28 that revealed different expression levels between good and poor responders to chemotherapy and appeared to be associated with chemosensitivity. On that basis, we developed a "Drug Response Scoring" system that was correlated well with individual sensitivity to an anticancer drug regimen. Among the 44 cases with positive drug-response scores by our definition, 40 achieved complete remission after treatment, whereas the only 3 of the 20 cases with negative scores responded well to the treatment. An ability to predict chemosensitivity should eventually lead to achievement of our goal of "personalized therapy."
authors.affiliation: Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Okutsu
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Tsunoda
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kaneta
authors.forename: Toyomasa
authors.initials: T
authors.lastname: Katagiri
authors.forename: Osamu
authors.initials: O
authors.lastname: Kitahara
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Zembutsu
authors.forename: Rempei
authors.initials: R
authors.lastname: Yanagawa
authors.forename: Shuichi
authors.initials: S
authors.lastname: Miyawaki
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Kuriyama
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kubota
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Kimura
authors.forename: Kohmei
authors.initials: K
authors.lastname: Kubo
authors.forename: Fumiharu
authors.initials: F
authors.lastname: Yagasaki
authors.forename: Toshio
authors.initials: T
authors.lastname: Higa
authors.forename: Hirokuni
authors.initials: H
authors.lastname: Taguchi
authors.forename: Tadasu
authors.initials: T
authors.lastname: Tobita
authors.forename: Hideki
authors.initials: H
authors.lastname: Akiyama
authors.forename: Akihiro
authors.initials: A
authors.lastname: Takeshita
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Toshiko
authors.initials: T
authors.lastname: Motoji
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Ohno
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D000328:Adult; D000368:Aged; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D016678:Genome; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D012313:RNA