title: Activation of the bradykinin-forming cascade on endothelial cells: a role for heat shock protein 90.
abstract: Bradykinin is a major mediator of swelling in C1 inhibitor deficiency as well as the angioedema seen with ACE inhibitors and may contribute to bronchial hyper-reactivity in asthma. Formation of bradykinin occurs in the fluid phase and along cell surfaces requiring interaction of Factor XII, prekallikrein and high molecular weight kininogen (HK). The mechanism by which initiation occurs is uncertain. Recent data suggest that activation of the kinin-forming cascade can occur on the surface of endothelial cells, even in the absence of Factor XII. We demonstrate herein that during a 2-h incubation time, plasma deficient in either Factor XII or high molecular weight kininogen (HK) fail to activate kinin-forming cascade as compared to normal plasma. With more prolonged incubation, Factor XII deficient plasma gradually activates and HK deficient plasma does not. Our data support both Factor XII-dependent (rapid) and Factor XII-independent (slow) mechanisms; the latter may require a cell-derived protein (possibly protease) to activate prekallikrein in the presence of zinc ion and HK. To further define this cellular factor, we demonstrated that both cytosolic and membrane fractions from endothelial cells possessed the ability to catalyze prekallikrein conversion to kallikrein in the presence of HK and zinc ion. We purified this factor from cytosol by affinity chromatography employing corn trypsin inhibitor (CTI) as ligand. The fractions with peak activity were subjected to SDS-PAGE analysis, ligand blotted with biotinylated CTI, and positive bands were sequenced. Heat shock protein 90 (Hsp90) was identified as one of the proteins. Zinc-dependent activation of the prekallikrein-HK complex on endothelial cells was inhibited upon the addition of polyclonal antibody to Hsp90 in a dose-dependent manner. Although the mechanism by which Hsp90 activates the kinin-forming cascade is not yet clear, this protein represents the cellular contribution to the reaction and may become the dominant mechanism in pathologic circumstances in which Hsp90 is highly expressed or secreted.
authors.affiliation: Department of Medicine, Division of Pulmonary and Critical Care, Allergy and Clinical Immunology and Konishi-MUSC, Institute for Inflammation Research, 96 Jonathan Lucas Street, P.O. Box 250623, Charleston, SC 29425, USA.
authors.forename: Kusumam
authors.initials: K
authors.lastname: Joseph
authors.forename: Baby G
authors.initials: BG
authors.lastname: Tholanikunnel
authors.forename: Allen P
authors.initials: AP
authors.lastname: Kaplan
mesh_terms: D000595:Amino Acid Sequence; D019921:Biotinylation; D001798:Blood Proteins / Q000378:metabolism*; D001920:Bradykinin / Q000378:metabolism*; D002352:Carrier Proteins; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005174:Factor XII / Q000378:metabolism; D015956:Factor XIIa / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D018960:Hyaluronan Receptors; D019679:Kininogen, High-Molecular-Weight / Q000378:metabolism; D008024:Ligands; D008562:Membrane Glycoproteins; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D011288:Prekallikrein / Q000378:metabolism; D011951:Receptors, Complement / Q000378:metabolism; D014471:Umbilical Veins / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
chemical_list: D001798:Blood Proteins; C087299:C1QBP protein, human; D002352:Carrier Proteins; D018841:HSP90 Heat-Shock Proteins; D018960:Hyaluronan Receptors; D019679:Kininogen, High-Molecular-Weight; D008024:Ligands; D008562:Membrane Glycoproteins; D024101:Mitochondrial Proteins; D011951:Receptors, Complement; C032402:complement 1q receptor; D005174:Factor XII; D011288:Prekallikrein; D015956:Factor XIIa; D015032:Zinc; D001920:Bradykinin