title: In vitro study of collagen coating of titanium implants for initial cell attachment.
abstract: The aim of this study was to examine the influence of collagen coating on titanium on the initial attachment of human gingival fibroblasts for the development of the implant with periimplant soft tissue attachment. The morphological changes of cultured human gingival fibroblasts were investigated by scanning electron microcopy (SEM). Four different surfaces, i.e. non-coated mirror-polished titanium, collagen-coated titanium, non-coated tissue-culture polystyrene, and collagen-coated polystyrene were examined. Collagen coating of titanium was effective for enhancing the initial cell attachment. It is expected that collagen coating of titanium implants will improve the attachment of the peri-implant soft tissue to titanium at early stages after the implantation. SEM observation revealed the morphological effect of collagen coating on both titanium and polystyrene surfaces. Many lamellipodia and filopodia were recognized on collagen-coated titanium or polystyrene. Collagen coating improved the activity of human gingival fibroblasts.
authors.affiliation: Department of Comprehensive Clinical Dentistry, Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo 2-870-1, Sakaecho-nishi, Matsudo, Chiba 271-8587, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Nagai
authors.forename: Tohru
authors.initials: T
authors.lastname: Hayakawa
authors.forename: Akira
authors.initials: A
authors.lastname: Fukatsu
authors.forename: Masafumi
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Masahiko
authors.initials: M
authors.lastname: Fukumoto
authors.forename: Fumio
authors.initials: F
authors.lastname: Nagahama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mishima
authors.forename: Masao
authors.initials: M
authors.lastname: Yoshinari
authors.forename: Kimiya
authors.initials: K
authors.lastname: Nemoto
authors.forename: Takao
authors.initials: T
authors.lastname: Kato
mesh_terms: D002448:Cell Adhesion; D002452:Cell Count; D048429:Cell Size; D002470:Cell Survival; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D015921:Dental Implants; D017267:Dental Prosthesis Design; D005347:Fibroblasts / Q000166:cytology; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011137:Polystyrenes / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D011137:Polystyrenes; D003094:Collagen; D014025:Titanium