A simplified method for the detection of Y chromosome microdeletions in infertile men using a multiplex sequence-tagged site-based amplification.
Sixteen sequence-tagged sites (STSs) were combined in five amplification reactions, to screen for deletions of DNA fragments located within the AZFa, AZFb, and AZFc regions of the Y chromosome. This multiplex strategy is fast and reliable, and most of the azoospermia-associated deletions reported so far are detected with this simplified method. Internal control STSs are included that allow discrimination between deletion and failure of amplification.
Charpenel
Christine
C
Laboratoire de Biochimie et Biologie Moléculaire, Hôpital R Poincaré, 92380 Garches, France.
Guillon
Anne-Sophie
AS
Dorson
Olivier
O
Boucly
Catherine
C
Mathieu
Bertille
B
Montagnon
Martine
M
Merlet
Françoise
F
Van Amerongen
Geneviève
G
Bisson
Jean Pierre
JP
de Mazancourt
Philippe
P
D001483:Base Sequence; D041322:Chromosomes, Human, Y; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D017384:Sequence Deletion; D016324:Sequence Tagged Sites