Structure of the oligomers obtained by enzymatic hydrolysis of the glucomannan produced by the plant Amorphophallus konjac.
Dimers and trimers obtained by enzymatic hydrolysis of the glucomannan produced by the plant Amorphophallus konjac were analysed in order to obtain information on the saccharidic sequences present in the polymer. The polysaccharide was digested with cellulase and beta-mannanase and the oligomers produced were isolated by means of size-exclusion chromatography. They were structurally characterised using electrospray mass spectrometry, capillary electrophoresis, and NMR. The investigation revealed that many possible sequences were present in the polymer backbone suggesting a Bernoulli-type chain.
Cescutti
Paola
P
Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università di Trieste, via L. Giorgieri 1, Italy.
Campa
Cristiana
C
Delben
Franco
F
Rizzo
Roberto
R
D031056:Amorphophallus / Q000737:chemistry*; D004187:Disaccharides / Q000032:analysis; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry* / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry*; D014312:Trisaccharides / Q000032:analysis
D004187:Disaccharides; D008351:Mannans; D009844:Oligosaccharides; D014312:Trisaccharides; C022901:(1-6)-alpha-glucomannan; D006026:Glycoside Hydrolases