title: Synthesis and initial evaluation of 17-(11)C-heptadecanoic acid for measurement of myocardial fatty acid metabolism.
abstract: Fatty acid oxidation defects are being increasingly identified as causes of abnormal heart function and sudden death in children. Children with medium-chain acyl-coenzyme A (acyl-CoA) dehydrogenase defects can metabolize fatty acids labeled in the carboxylic acid end of the compound. Accordingly, our goal was to label a long-chain fatty acid in the omega-position and evaluate its myocardial kinetics.
  
  
  METHODS
  Heptadecanoic acid, a 17-carbon fatty acid, was labeled in the C-17 position with (11)C by the general process of coupling (11)C-methyliodide to t-butyl-15-hexadecanoate. Yield was approximately 5%-10% end-of-bombardment. Subsequently, evaluation studies were performed on isolated perfused rat hearts and in intact, anesthetized dogs. The myocardial uptake and efflux of 17-(11)C-heptadecanoic acid were compared with those of 1-(11)C-palmitate.
  
  
  RESULTS
  With the exception of delayed efflux of tracer reflecting the temporal delay for beta-oxidation, the washout of 17-(11)C-heptadecanoic acid from the heart mirrored that of 1-(11)C-palmitate in isolated rat hearts and in intact dogs with PET.
  
  
  CONCLUSIONS
  17-(11)C-Heptadecanoic acid may be a useful tracer for the identification of defects in fatty acid metabolism in subjects with medium- and short-chain fatty acid oxidation defects.
authors.affiliation: Division of Cardiology, Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA. tc311@columbia.edu
authors.forename: T Lee
authors.initials: TL
authors.lastname: Collier
authors.forename: Yuying
authors.initials: Y
authors.lastname: Hwang
authors.forename: Ravichandran
authors.initials: R
authors.lastname: Ramasamy
authors.forename: Robert R
authors.initials: RR
authors.lastname: Sciacca
authors.forename: Kathleen T
authors.initials: KT
authors.lastname: Hickey
authors.forename: Norman R
authors.initials: NR
authors.lastname: Simpson
authors.forename: Steven R
authors.initials: SR
authors.lastname: Bergmann
mesh_terms: D000818:Animals; D002250:Carbon Radioisotopes; D004285:Dogs; D005227:Fatty Acids / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D014055:Tomography, Emission-Computed
chemical_list: D002250:Carbon Radioisotopes; D005227:Fatty Acids; C013102:margaric acid