S100A8 and S100A9 calcium-binding proteins: localization within normal and cyclosporin A-induced overgrowth gingiva.
S100A8 and S100A9, also called myeloid related protein (MRP) 8 and 14, are calcium-binding proteins highly expressed in neutrophils, in which they play a key role in the inflammatory progression. In this study, we looked at the expression of S100A8/A9 within gingiva from normal and Cyclosporin A (CsA)-induced overgrowth gingiva. In gingiva from the CsA group, several positive S100A8/A9 cells were seen within the connective tissue, whereas in normal gingiva very few positive S100A8/A9 cells were detected. These cells correspond either to activated macrophages or to neutrophils, reflecting the well-known gingival inflammatory status associated with the CsA-treated group. In addition, in both the normal and drug-treated group, the gingival epithelia appeared S100A8/A9 immunopositive. More specifically, S100A8/A9 appeared in the majority within the spino-cellular layer and located extracellularly within the desmosomes. In addition, S100A8/A9 also appeared sporadically intracellularly, located within the cytoplasm and the nuclei, reflecting S100A8/A9 translocations.
Echelard
S
S
Laboratory of Histopathology, Free University of Brussels, Head and Neck Center, Department of Stomatology and Maxillo-Facial Surgery, Erasme Hospital, Brussels, Belgium.
Hoyaux
D
D
Hermans
M
M
Daelemans
P
P
Roth
J
J
Philippart
P
P
Pochet
R
R
D000328:Adult; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D040501:Calgranulin A / Q000378:metabolism*; D040502:Calgranulin B / Q000378:metabolism*; D016572:Cyclosporine / Q000009:adverse effects*; D005260:Female; D005881:Gingiva / Q000378:metabolism; D019214:Gingival Overgrowth / Q000139:chemically induced* / Q000378:metabolism*; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D014018:Tissue Distribution
D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D040502:Calgranulin B; D007166:Immunosuppressive Agents; D016572:Cyclosporine