title: Rapid effects of neurotrophic factors on calcium homeostasis in rat visceral afferent neurons.
abstract: Neurotrophic factors maintain and modulate neuron function in adults. We tested the hypothesis that neurotrophic factors rapidly alter intracellular calcium concentrations, thereby affecting neuron excitability. The majority of rat nodose neurons express TrkA, TrkB and TrkC receptor after 1 day in culture. Addition of nerve growth factor, brain derived neurotrophic factor or glial derived neurotrophic factor increased cytosolic calcium in about one third of the neurons within less than 10 min. This increase was due to calcium release from intracellular stores and could be blocked by the tyrosine kinase inhibitor K252a. The rapid effect of neurotrophic factors suggests a role of these molecules in the early response after inflammation as potential mediators for sensitization of afferent neurons.
authors.affiliation: Department of Internal Medicine, University of Iowa, 4614 JCP, 200 Hawkins Drive, Iowa City, IA 52242, USA.
authors.forename: Kenneth
authors.initials: K
authors.lastname: Lamb
authors.forename: Klaus
authors.initials: K
authors.lastname: Bielefeldt
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D016257:Fura-2; D051100:Glial Cell Line-Derived Neurotrophic Factor; D006706:Homeostasis / Q000187:drug effects* / Q000502:physiology; D008297:Male; D020932:Nerve Growth Factor / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D009620:Nodose Ganglion / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020917:Receptor, trkA / Q000187:drug effects / Q000378:metabolism; D020813:Receptor, trkB / Q000187:drug effects / Q000378:metabolism; D020812:Receptor, trkC / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D017833:Visceral Afferents / Q000187:drug effects* / Q000378:metabolism
chemical_list: C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D020932:Nerve Growth Factor; D020917:Receptor, trkA; D020813:Receptor, trkB; D020812:Receptor, trkC; D002118:Calcium; D016257:Fura-2