title: Cytology of morcellated renal specimens: significance in diagnosis and dissemination.
abstract: OBJECTIVE
  Controversy surrounds the process of morcellation for retrieving laparoscopically removed specimens. The inability to assess tumor stage, increased difficulty in pathological examination and the potential for tumor spillage are cited as significant disadvantages of the technique. We examined cytological findings in bag washings after laparoscopic nephrectomy for benign and malignant diseases.
  
  
  METHODS
  We prospectively obtained cytology washings from the retrieval bag after laparoscopic nephrectomy and manual morcellation. In 22 consecutive cases after specimen fragmentation in a LapSac (Cook Urological, Spencer, Indiana) the bag was thoroughly irrigated with 30 cc normal saline. This wash was then processed by ThinPrep (Cytyc Corp., Marlborough, Massachusetts) and stained with Papanicolaou stain. Standard pathological examination of the morcellated specimen was performed to determine renal histology.
  
  
  RESULTS
  The histological diagnosis was clear cell renal carcinoma in 10 cases, multicystic renal carcinoma in 2, papillary renal cell carcinoma in 1, angiomyolipoma in 1, and oncocytoma in 1. Bag cytological results were accurate in 9 of 13 patients with carcinoma (69%), while in 3 cytological study provided additional information. In all 9 cases of benign histology, cytological findings were consistent with benign cellular features. Neoplastic cells were easily detected and classified into type and grade.
  
  
  CONCLUSIONS
  Cytological examination of LapSac washings after specimen morcellation provided a pathological diagnosis in the majority of patients. This method may complement existing techniques and be useful for increasing the accuracy of pathological analysis of morcellated specimens. In addition, these data suggest that malignant cells are liberated during the morcellation process, which has significant implications for potential tumor dissemination.
authors.affiliation: Department of Urology, University of California School of Medicine, San Francisco, CA, USA.
authors.forename: Maxwell V
authors.initials: MV
authors.lastname: Meng
authors.forename: Theodore R
authors.initials: TR
authors.lastname: Miller
authors.forename: Imok
authors.initials: I
authors.lastname: Cha
authors.forename: Marshall L
authors.initials: ML
authors.lastname: Stoller
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003581:Cytodiagnosis; D005260:Female; D006801:Humans; D007668:Kidney / Q000473:pathology*; D007680:Kidney Neoplasms / Q000175:diagnosis; D010535:Laparoscopy / Q000009:adverse effects; D008297:Male; D008875:Middle Aged; D009366:Neoplasm Seeding; D009392:Nephrectomy; D011446:Prospective Studies; D013048:Specimen Handling