[Diagnosis of tuberculosis by immunocapture of the tuberculous bacillus (using magnetic beads)].
Tuberculosis is worldwide considered as a major health problem with high morbidity and mortality rates. Diagnosis of tuberculosis can be problematic. Microscopy, as the basic diagnostic method, stands inadequately alone due to a low sensitivity, and culture suffers from being time-consuming. A rapid, sensitive and simple diagnostic test, applicable in the field is therefore highly needed. A diagnostic method for the detection of M. tuberculosis by immunocapture technique has been developed using magnetic beads coated with polyclonal anti-M. tuberculosis. The detection of captured bacilli using biotinylated anti-APA monoclonal antibody (APA is a minor secreted antigen) was found more sensitive than microscopy. The results suggest that the development of a rapid strip test to detect major antigen could be a useful tool for the control of tuberculosis.
Rabe
O
O
Institut Pasteur de Madagascar, BP 1274, 101 Antananarivo, Madagascar.
Chanteau
S
S
Marchal
G
G
Rasolofo Razanamparany
V
V
D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001706:Biopsy; D016022:Case-Control Studies; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods* / Q000592:standards; D008270:Madagascar; D008853:Microscopy / Q000592:standards; D009169:Mycobacterium tuberculosis / Q000276:immunology; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology; D013997:Time Factors; D014376:Tuberculosis / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000382:microbiology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; C072681:Mycobacterium tuberculosis antigens