Protective immunity against Taenia crassiceps murine cysticercosis induced by DNA vaccination with a Taenia saginata tegument antigen.
This study investigated the protective capacity of the recombinant Taenia saginata Tso18 antigen administered as a DNA vaccine in the Taenia crassiceps murine model of cysticercosis. This Tso18 DNA sequence, isolated from a T. saginata oncosphere cDNA library, has homologies with Taenia solium and Echinococcus sp. It was cloned in the pcDNA3.1 plasmid and injected once intramuscularly into mice. Compared to saline-vaccinated control mice, immunization reduced the parasite burden by 57.3-81.4%, while lower levels of non-specific protection were induced in control mice injected with the plasmid pcDNA3.1 (18.8-33.1%) or a plasmid with irrelevant construct, pcDNA3.1/3D15 (33.4-38.8%). Importantly, significant levels of protection were observed between the pcDNA3.1/Tso18 plasmid and pcDNA3.1/3D15 plasmid immunized mice. Mice immunized with pTso18 synthesized low levels of, primarily IgG1 sub-class, antibodies. These antibodies were shown to recognize a 66 kDa antigen fraction of T. crassiceps and T. solium. Splenocytes enriched in both CD4+CD8- and CD4-CD8+ T cells from these vaccinated mice proliferated in vitro when exposed to antigens from both T. solium and T. crassiceps cestodes. Immunolocalization studies revealed the Tso18 antigen in oncospheres of T. saginata and T. solium, in the adult tapeworm and in the tegument of T. solium cysticerci. The protective capacity of this antigen and its extensive distribution in different stages, species and genera of cestodes points to the potential of Tso18 antigen for the possible design of a vaccine against cestodes.
Rosas
Gabriela
G
Facultad de Medicina, Unidad de Biomedicina Molecular, Universidad Autónoma del Estado de Morelos, 62210 Cuernavaca, Morelos, Mexico.
Fragoso
Gladis
G
Garate
Teresa
T
Hernández
Beatriz
B
Ferrero
Patricia
P
Foster-Cuevas
Mildred
M
Parkhouse
R Michael E
RM
Harrison
Leslie J S
LJ
Briones
Sergio López
SL
González
Luis Miguel
LM
Sciutto
Edda
E
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002478:Cells, Cultured; D003429:Cross Reactions; D003551:Cysticercosis / Q000276:immunology / Q000517:prevention & control*; D018509:DNA, Helminth / Q000235:genetics / Q000276:immunology*; D008018:Life Cycle Stages / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology; D013621:Taenia / Q000276:immunology*; D041202:Taenia saginata / Q000276:immunology*; D014611:Vaccination; D019444:Vaccines, DNA
D000947:Antigens, Helminth; D018509:DNA, Helminth; D019444:Vaccines, DNA