High-performance liquid chromatographic separation and on-line mass spectrometric detection of saturated and unsaturated oligogalacturonic acids.
A method for the simultaneous determination of saturated and unsaturated oligogalacturonic acids up to degree of polymerization (dp) of 7 by high-performance liquid chromatography (HPLC) is presented. For this purpose, a Cyclobond I 2000 column and a volatile mobile phase consisting of ammonium formate and methanol were used, allowing direct coupling of HPLC to a mass spectrometer via an electrospray interface (ESI-MS) without additional desalting. The analytical system was used for the characterization of digests obtained by incubation of polygalacturonic acid with commercial enzyme preparations.
Stoll
Thomas
T
Institute of Food Technology, Section Plant Foodstuff Technology, Hohenheim University, Garbenstrasse 25, D-70599 Stuttgart, Germany.
Schieber
Andreas
A
Carle
Reinhold
R
D002851:Chromatography, High Pressure Liquid; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D009862:Online Systems; D021241:Spectrometry, Mass, Electrospray Ionization
D009844:Oligosaccharides; C412763:oligogalacturonic acid; D006026:Glycoside Hydrolases