title: Antibodies against beta 2-glycoprotein I (beta 2-GP I) and their relationship to fetoplacental antigens.
abstract: Binding of beta 2-GP I to anionic phospholipids is thought to be the major antigen required in the reaction of anticardiolipin antibodies to phospholipids. The aim of this study was to investigate the changes of anti-beta 2-GP I IgG during the first and second trimester of pregnancy and the relationship between the levels of anti-beta 2-GP I and fetoplacental antigens and the correlation between anti-beta 2-GP I IgG and antibodies against oxidized low-density lipoprotein IgG (oLAb) in serum of pregnant women. We determined anticardiolipin antibodies (ACA) IgG and maternal serum levels of alpha 1-fetoprotein (AFP), human chorionic gonadotrophin (HCG) and trophoblast-specific beta 1-glycoprotein (SP1) in 204 pregnant women in the first and second trimester. From this group we selected 52 serum samples positive for ACA IgG and 16 samples negative for ACA IgG. In the samples of selected patients, the levels of anti-beta 2-GP I IgG and oLAb IgG were determined. Anti-beta 2-GP I IgG levels significantly decreased in the second trimester (6.2+/-9.3 U/ml, mean +/- S.D.) in comparison with the first trimester (8.3+/-10.4 U/ml) (p=0.05). Multiple of median (MoM) AFP correlated negatively but not significantly in the first trimester with anti-beta 2-GP I (r = -0.261, p = 0.12). In the second trimester this correlation was significantly negative (r = -0.278, p = 0.04). The Spearman correlation coefficients for MoM HCG and anti-beta 2-GP I were 0.158 for the first trimester and 0.174 for the second trimester. MoM SP1 also did not correlate significantly with anti-beta 2-GP I in both trimesters. The correlation between anti-beta 2-GP I IgG and oLAb IgG was not significant (r = -0.06). In the first trimester 40 % serum samples were positive for anti-beta 2-GP I IgG and negative for oLAb IgG or vice versa, while 60 % samples in the second trimester were positive only for one determined autoantibody. We can conclude that the levels of anti-beta 2-GP I IgG decrease during the second trimester probably as the result of the effects of some immunosuppressive agents associated with pregnancy. The finding of negative correlation between AFP and anti-beta 2-GP I suggests that anti-beta 2-GP I has an influence on fetus development.
authors.affiliation: First Institute of Medical Chemistry and Biochemistry, Institute of Clinical Chemistry, First Medical Faculty, Charles University, Prague, Czech Republic. lFial@lf1.cuni.cz
authors.forename: L
authors.initials: L
authors.lastname: Fialová
authors.forename: I
authors.initials: I
authors.lastname: Malhoban
authors.forename: L
authors.initials: L
authors.lastname: Mikulíková
authors.forename: O
authors.initials: O
authors.lastname: Benesová
authors.forename: A
authors.initials: A
authors.lastname: Zwinger
mesh_terms: D017152:Antibodies, Antiphospholipid / Q000097:blood*; D006063:Chorionic Gonadotropin / Q000097:blood / Q000276:immunology; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008431:Maternal-Fetal Exchange / Q000276:immunology; D010920:Placenta / Q000276:immunology*; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000276:immunology; D011262:Pregnancy Trimester, Second / Q000276:immunology; D000509:alpha-Fetoproteins / Q000276:immunology* / Q000378:metabolism; D053482:beta 2-Glycoprotein I
chemical_list: D017152:Antibodies, Antiphospholipid; D006063:Chorionic Gonadotropin; D006023:Glycoproteins; D007074:Immunoglobulin G; D000509:alpha-Fetoproteins; D053482:beta 2-Glycoprotein I