title: Expression of CD44V6 and PCNA in squamous cell carcinomas.
abstract: OBJECTIVE
  To investigate the expression of cluster of differentiation 44 variant 6 (CD(44V6)) and proliferating cell nuclear antigen (PCNA) in ocular squamous cell carcinomas.
  
  
  METHODS
  Streptavidin-biotin complex (SABC) immunohistochemistry was used to explore the expression of CD(44V6) and PCNA in 35 cases of ocular squamous cell carcinomas, 20 cases of papillomas, and 11 cases of normal eyelid tissue.
  
  
  RESULTS
  The CD(44V6) positive rate was 62.9% (22/35) in ocular squamous cell carcinomas, 15.0% (3/20) in papillomas, but not detectable in the 11 cases of normal eyelid tissue. The positive expression rates of CD(44V6) in ocular squamous cell carcinomas were significantly higher than in benign tumors (chi(2) = 11.57, P < 0.01) or control tissue (P = 0.001), and the positive expression rates of CD(44V6) in metastasis were significantly higher than without metastasis (P = 0.049). PCNA labeling indexes (PI) in tumors with CD(44V6) expression were significantly higher than those without (t = 20.21, P < 0.01).
  
  
  CONCLUSIONS
  Overexpression of CD(44V6) is correlated with the progress and metastasis of ocular squamous cell carcinomas. CD(44V6) protein positive staining is associated with high PI. CD(44V6) and PCNA are useful for evaluating prognosis.
authors.affiliation: Department of Ophthalmology, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China.
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Niu
authors.forename: Fuling
authors.initials: F
authors.lastname: Liu
authors.forename: Zhanyu
authors.initials: Z
authors.lastname: Zhou
authors.forename: Hongyun
authors.initials: H
authors.lastname: Wang
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000473:pathology; D005134:Eye Neoplasms / Q000737:chemistry* / Q000473:pathology; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis*; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D012867:Skin / Q000737:chemistry
chemical_list: C112697:CD44v6 antigen; D006023:Glycoproteins; D018960:Hyaluronan Receptors; D018809:Proliferating Cell Nuclear Antigen