Expression of antisense thrombin receptor gene inhibits intimal hyperplasia of rat carotid artery after balloon injury.
OBJECTIVE
To study the mechanism of restenosis after angioplasty and to clarify the effect of thrombin and its receptor on restenosis development.


METHODS
Balloon catheter-induced injury was adopted to induce intimal hyperplasia of the carotid arteries in rats. Antisense thrombin receptor (ATR) cDNA was transfected by perfusing recombinant LXSN ATR plasmid/nanoparticle complex into the segment of the injured carotid artery.


RESULTS
PCR result showed integration of the recombined gene. Dot blot showed the expression of antisense TR mediated by recombinant LXSN ATR plasmid/nanoparticle complex in the wall of common carotid arteries of the experimental group rats, which enabled to inhibit TR gene expression and intimal hyperplasia of the injured arteries.


CONCLUSIONS
Thrombin and its receptor play an important role in the formation of neointima after the injury, which provides a potential clue in developing a new approach for prevention and treatment of restenosis after angioplasty.
Ren
Guofeng
G
Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China.
Wang
Zongli
Z
Li
Yongjun
Y
Yang
Jing
J
Liu
Peimao
P
She
Mingpeng
M
D000818:Animals; D002339:Carotid Arteries; D006965:Hyperplasia; D051381:Rats; D018179:Receptors, Thrombin / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology; D017539:Tunica Intima / Q000378:metabolism
D018179:Receptors, Thrombin; D013917:Thrombin