Voltage-gated mobility of the Ca2+ channel cytoplasmic tails and its regulatory role.
Transient increase in intracellular free Ca(2+) concentration generated by the voltage-gated Ca(v)1.2 channels acts as an important intracellular signal. By using fluorescence resonance energy transfer combined with patch clamp in living cells, we present evidence for voltage-gated mobility of the cytoplasmic tails of the Ca(v)1.2 channel and for its regulatory role in intracellular signaling. Anchoring of the C-terminal tail to the plasma membrane caused an inhibition of its state-dependent mobility, channel inactivation, and CREB-dependent transcription. Release of the tail restored these functions suggesting a direct role for voltage-gated mobility of the C-terminal tail in Ca(2+) signaling.
Kobrinsky
Evgeny
E
National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA.
Schwartz
Elena
E
Abernethy
Darrell R
DR
Soldatov
Nikolai M
NM
D000818:Animals; D019556:COS Cells; D020746:Calcium Channels, L-Type / Q000032:analysis* / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D015640:Ion Channel Gating; D017434:Protein Structure, Tertiary; D015398:Signal Transduction
D020746:Calcium Channels, L-Type; C419385:L-type calcium channel alpha(1C)