Isolation of vascular smooth muscle cells from a single murine aorta.
The vascular smooth muscle cell plays a significant role in many important cardiovascular disorders, and smooth muscle biology is therefore important to cardiovascular research. The mouse is critical to basic cardiovascular research, largely because techniques for genetic manipulation are more fully developed in the mouse than in any other mammalian species. We describe here a technique for isolating smooth muscle cells from a single mouse aorta. This technique is particularly useful when material is limiting, as is frequently the case when genetically modified animals are being characterized.
Ray
J L
JL
Cardiovascular Research Center, University of Michigan Medical School, Department of Internal Medicine, Ann Arbor, MI, USA.
Leach
R
R
Herbert
J M
JM
Benson
M
M
D000199:Actins / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000166:cytology*; D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins / Q000378:metabolism; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D017364:Collagenases; D007150:Immunohistochemistry; D008164:Luminescent Proteins; D051379:Mice; D008822:Mice, Transgenic; D008840:Microfilament Proteins / Q000235:genetics; D009124:Muscle Proteins / Q000235:genetics; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D015203:Reproducibility of Results; D000096985:Calponins
D000199:Actins; D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins; D017364:Collagenases; D008164:Luminescent Proteins; D008840:Microfilament Proteins; D009124:Muscle Proteins; D000096985:Calponins; C058992:Tagln protein, mouse; C051615:transgelin; C054562:yellow fluorescent protein, Bacteria