title: [Ultrastructural observation of normal spermatozoa incubated with ROS in vitro].
abstract: OBJECTIVE
  To observe ultrastructural changes of spermatozoa after incubate with reactive oxygen species (ROS).
  
  
  METHODS
  Spermatozoa of normal physiological functions selected from semen samples by Percoll gradient centrifugation technique were regarded as normal sperm models in present study. Ultrastructural changes of spermatozoa observed by transmission electron microscope after model spermatozoa were incubated with ROS generated by hypoxanthine and xanthine oxidase under aerobic environment.
  
  
  RESULTS
  After model spermatozoa were incubated with ROS, impairment of various extent in membrane and acrosome of spermatozoa and abnormality in mitochondria of spermatozoa were found.
  
  
  CONCLUSIONS
  Excessive ROS may cause ultrastructural change in membrane, acrosome and mitochondria of spermatozoa and impair function of spermatozoa.
authors.affiliation: Laboratory of Reproduction & Genetics, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Shang
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Huang
authors.forename: Cheng-Liang
authors.initials: CL
authors.lastname: Xiong
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Yin
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Wang
mesh_terms: D000328:Adult; D006801:Humans; D008297:Male; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D013094:Spermatozoa / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D017382:Reactive Oxygen Species