title: Kinetic model for the inactivation of Lactobacillus plantarum by pulsed electric fields.
abstract: The kinetics of Lactobacillus plantarum inactivation by pulsed electric fields (PEF) was studied in two different growth stages (exponential and stationary), but in the same reference medium (0.6% peptone water). Electric field intensity and treatment time varied from 20 to 28 kV/cm and 30 to 240 micros, respectively. The experimental data showed that cells in the exponential growth stage were more sensitive to PEF treatment than those in the stationary stage. The inactivation data were adjusted to the Bigelow and Hülsheger models and the Weibull frequency distribution function, and constants were calculated for both growth stages in each model. The models were tested and their accuracy was assessed by using the Accuracy Factor. According to this parameter, the Weibull frequency distribution function gave better fittings for the inactivation by PEF than Bigelow or Hülsheger models. On the other hand, the Bigelow model gave a good accuracy factor and is simpler.
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos, C.S.I.C., Apartado de Correos 73, 46100 Burjassot, Valencia, Spain. mrodrigo@iata.csic.es
authors.forename: D
authors.initials: D
authors.lastname: Rodrigo
authors.forename: P
authors.initials: P
authors.lastname: Ruíz
authors.forename: G V
authors.initials: GV
authors.lastname: Barbosa-Cánovas
authors.forename: A
authors.initials: A
authors.lastname: Martínez
authors.forename: M
authors.initials: M
authors.lastname: Rodrigo
mesh_terms: D004558:Electric Stimulation; D004560:Electricity; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D007700:Kinetics; D007778:Lactobacillus / Q000254:growth & development*; D008954:Models, Biological; D011673:Pulsatile Flow; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016008:Statistical Distributions