[Influence of low molecular weight heparin on proliferation of bovine lens epithelial cells on the surface of intraocular lens].
OBJECTIVE
To investigate the anti-proliferation effect of low molecular weight heparin (LMWH) on bovine lens epithelial cells (BLEC) on the surface of polymethylmethacrylate (PMMA) intraocular lens (IOL).


METHODS
BLECs were incubated in vitro on the surface of PMMA IOLs. The cultural media contained LMWH (1 x 10(5) U/L) or normal heparin (1 x 10(5) U/L) or none of them (control). Cells were counted by flow cytometry.


RESULTS
The heparin or LMWH treated BLECs on the surface of PMMA IOLs showed immature appearance, while the BLECs in the control group showed a tendency of fibrosis. The numbers of BLECs (per ml) in the digested solution from the surface of IOLs were 878.2 +/- 107.8 (control), 545.5 +/- 58.2 (heparin) and 407.8 +/- 36.6 (LMWH). The comparisons among the three groups showed significant differences (P < 0.001).


CONCLUSIONS
Low molecular weight heparin inhibits proliferation of lens epithelial cells on the surface of intraocular lens, which could be used as a potential drug to prevent the occurrence of postoperative capsular opacification after IOL implantation.
Wu
Renyi
R
Department of Ophthalmology, Affiliated Second Hospital, Medical College of Zhejiang University, Hangzhou 310009, China.
Yao
Ke
K
Ye
Juan
J
Xu
Wen
W
Sun
Zhaohui
Z
Shentu
Xingchao
X
D000818:Animals; D002417:Cattle; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects*; D005434:Flow Cytometry; D006495:Heparin, Low-Molecular-Weight / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects; D007910:Lenses, Intraocular; D019904:Polymethyl Methacrylate
D006495:Heparin, Low-Molecular-Weight; D019904:Polymethyl Methacrylate