Effect of undernutrition on GMP-PNP binding and adenylate cyclase activity from rat brain.
1. Undernutrition is an insult that affects brain development and functioning. Considering that signaling through metabotropic receptors/G proteins is critical for normal synaptic transmission and contributes to CNS development and synaptic plasticity, the present study investigated the effects of pre- and postnatal protein deprivation (diet: 8% protein; normonourished group: 25% protein) on brain signal transduction by G proteins. 2. Undernutrition decreased the [3H] GMP-PNP binding to G proteins and AC activity, in neural plasma synaptic membranes of 21- and 75-day-old rats. This effect was less pronounced or even absent in old rats. 3. Ontogenetically, the dietary treatment effect might be interpreted as a retarded development associated with protein malnutrition.
Rotta
Liane N
LN
Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Nogueira
Cristina W
CW
da Silva
Luciane
L
Soares
Félix A
FA
Perry
Marcos L S
ML
Souza
Diogo O
DO
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001665:Binding Sites / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000196:embryology* / Q000201:enzymology / Q000254:growth & development; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology*; D004044:Dietary Proteins / Q000378:metabolism; D004622:Embryo, Mammalian; D005260:Female; D005508:Food Deprivation / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006157:Guanosine Monophosphate / Q000378:metabolism*; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D008297:Male; D011247:Pregnancy; D011488:Protein Deficiency / Q000150:complications / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D018094:Receptors, Metabotropic Glutamate / Q000378:metabolism*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology
D004044:Dietary Proteins; D018094:Receptors, Metabotropic Glutamate; D006165:Guanylyl Imidodiphosphate; D006157:Guanosine Monophosphate; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases