Mcm1 binds replication origins.
Mcm1 is an essential protein required for the efficient replication of minichromosomes and the transcriptional regulation of early cell cycle genes in Saccharomyces cerevisiae. In this study, we report that Mcm1 is an abundant protein that associates globally with chromatin in a punctate pattern. We show that Mcm1 is localized at replication origins and plays an important role in the initiation of DNA synthesis at a chromosomal replication origin in vivo. Using purified Mcm1 protein, we show that Mcm1 binds cooperatively to multiple sites at autonomously replicating sequences. These results suggest that, in addition to its role as a transcription factor for the expression of replication genes, Mcm1 may influence the local structure of replication origins by direct binding.
Chang
Victoria K
VK
Department of Chemistry, Drew University, Madison, New Jersey 07940, USA.
Fitch
Michael J
MJ
Donato
Justin J
JJ
Christensen
Tim W
TW
Merchant
A Margaret
AM
Tye
Bik K
BK
D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004261:DNA Replication / Q000235:genetics*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D026341:Minichromosome Maintenance 1 Protein / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D018741:Replication Origin / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D004271:DNA, Fungal; D026341:Minichromosome Maintenance 1 Protein; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D005982:Glutathione Transferase