Cutting edge: differential chemokine production by myeloid and plasmacytoid dendritic cells.
To examine the different roles of myeloid dendritic cells (M-DCs) and plasmacytoid dendritic cells (P-DCs) in the induction and regulation of immune response, we have studied chemokine secretion by freshly isolated DC subsets in response to bacterial, viral, and T cell-derived stimuli. M-DCs selectively produced very high levels of the homeostatic chemokines CC chemokine ligand (CCL)17 and CCL22, while P-DCs produced very little if any. In contrast, the proinflammatory chemokine CCL3 was secreted mostly by P-DCs, whereas CCL4 and CXC chemokine ligand 8 were produced by both subsets. The selective production of CCL17 and CCL22 by M-DCs but not P-DCs was confirmed in vivo by immunohistology on human reactive lymph node sections. The high production of CCR4 ligands by M-DCs suggests their capacity to selectively recruit at sites of inflammation T cells with regulatory properties or with a Th2 phenotype, whereas P-DCs, by preferentially secreting CCR1/CCR5 ligands, would mostly recruit effector T cells and, in particular, Th1-type cells.
Penna
Giuseppe
G
BioXell, Milano, Italy.
Vulcano
Marisa
M
Roncari
Andrea
A
Facchetti
Fabio
F
Sozzani
Silvano
S
Adorini
Luciano
L
D019070:Cell Lineage / Q000276:immunology; D002478:Cells, Cultured; D054414:Chemokine CCL17; D054422:Chemokine CCL22; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018925:Chemokines / Q000096:biosynthesis* / Q000097:blood; D019742:Chemokines, CC / Q000096:biosynthesis; D003713:Dendritic Cells / Q000145:classification / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis; D022423:Myeloid Cells / Q000276:immunology* / Q000378:metabolism*
C106230:CCL17 protein, human; C515482:CCL22 protein, human; D054414:Chemokine CCL17; D054422:Chemokine CCL22; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018925:Chemokines; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins