[Detection of leptospirosis reservoirs in Madagascar using the polymerase chain reaction technique].
A polymerase chain reaction (PCR) technique was used for detection of the Leptospira interrogans rrs gene in kidney tissue from 115 rats, 50 zebu cattles and 13 pigs in an attempt to identify a possible animal reservoir of leptospirosis in Madagascar. In addition, serological testing of 105 individuals in close contact with animals was carried out. The PCR analysis was negative for all the samples tested and only one person was found seropositive at a low titer. The findings suggest that leptospirosis, if prevalent in Madagascar, is likely rare.
Ralaiarijaona
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Institut Pasteur de Madagascar, BP 1274, 101, Antananarivo, Madagascar.
Bellenger
E
E
Chanteau
S
S
Roger
F
F
Pérolat
P
P
Rasolofo Razanamparany
V
V
D000818:Animals; D001431:Bacteriological Techniques; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D004197:Disease Reservoirs / Q000662:veterinary*; D004199:Disease Vectors; D006801:Humans; D007921:Leptospira interrogans / Q000235:genetics*; D007922:Leptospirosis / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D008270:Madagascar / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011159:Population Surveillance / Q000379:methods; D015995:Prevalence; D051381:Rats; D012376:Rodent Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D015047:Zoonoses / Q000453:epidemiology / Q000382:microbiology
D004269:DNA, Bacterial