title: Establishment of an immortalized cell line and transplantable xenograft from a bronchioloalveolar lung carcinoma of a cat.
abstract: OBJECTIVE
  To establish an immortalized cell line and transplantable xenograft of feline bronchioloalveolar lung carcinoma (BAC).
  
  
  METHODS
  Pleural effusion from a 12-year-old Persian male cat with BAC.
  
  
  METHODS
  Tumor cells from the pleural effusion were grown in monolayer cell culture and injected into severe combined immunodeficient (SCID) mice to establish an immortalized cell line as well as a transplantable xenograft.
  
  
  RESULTS
  Both the primary lung carcinoma, the derived cell line, and the transplantable xenograft had evidence of a type-II pneumocyte origin expressing lamellar bodies ultrastructurally and thyroid transcription factor-1 and surfactant immunocytochemically. All 3 also expressed nuclear p53 immunoreactivity. A metaphase spread of the cell line (SPARKY) probed with fluorescein-labeled genomic feline DNA gave evidence of its feline origin. Flow cytometric studies indicated aneuploidy with a DNA index of 1.6. An R-banded karyotype revealed a modal number of 66 including the feline Y chromosome. The cell line had a doubling time of 16 hours. The xenograft (SPARKY-X) reached a diameter of 1 cm in 3 weeks in SCID mice. Deoxyribonucleic acid fingerprint analysis revealed that SPARKY and SPARKY-X were novel and strongly matched each other, except for the murine component found in SPARKY-X. Interestingly, SPARKY-X manifested the characteristic lepidic growth pattern of pulmonic BAC.
  
  
  CONCLUSIONS
  Both the cell line and xenograft retained their autochthonous BAC phenotype, making them useful for the subsequent dissection of molecular abnormalities in feline BAC and in vitro screening of chemotherapeutic agents.
authors.affiliation: Department of Pathology, UCLA School of Medicine, Los Angeles, CA 90024, USA.
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Grossman
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: McNiel
authors.forename: Timothy B
authors.initials: TB
authors.lastname: Hackett
authors.forename: Sanford H
authors.initials: SH
authors.lastname: Barsky
mesh_terms: D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000473:pathology / Q000648:ultrastructure / Q000662:veterinary*; D000818:Animals; D001059:Apoproteins / Q000378:metabolism; D002371:Cat Diseases / Q000473:pathology*; D002415:Cats; D020732:Cytogenetic Analysis / Q000662:veterinary; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D007150:Immunohistochemistry / Q000662:veterinary; D008175:Lung Neoplasms / Q000473:pathology / Q000648:ultrastructure / Q000662:veterinary*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D009687:Nuclear Proteins / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D011003:Ploidies; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000378:metabolism; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000378:metabolism; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D001059:Apoproteins; D004273:DNA, Neoplasm; C000614414:Nkx2-1 protein, mouse; D009687:Nuclear Proteins; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; C046616:pulmonary surfactant apoprotein