Complete mitochondrial DNA sequence of a tadpole shrimp (Triops cancriformis) and analysis of museum samples.
The complete mitochondrial DNA (mtNDA) of the tadpole shrimp Triops cancriformis was sequenced. The sequence consisted of 15,101 bp with an A+T content of 69%. Its gene arrangement was identical with those sequences of the water flea (Daphnia pulex) and giant tiger prawn (Penaeus monodon), whereas it differed from that of the brine shrimp (Artemia franciscana) in the arrangement of its genes for tRNAs. Phylogenetic analysis revealed T. cancriformis to be more closely related to the water flea than to the brine shrimp and giant tiger prawn. We also compared the 16S rRNA sequences of five formalin-fixed tadpole shrimps that had been collected in five different locations and stored in a museum. The sequence divergence was in the range of 0-1.51%, suggesting that those samples were closely related to each other.
Umetsu
Kazuo
K
Department of Forensic Medicine, Yamagata University School of Medicine, Yamagata, Japan. kumetsu@med.id.yamagata-u.ac.jp
Iwabuchi
Naruki
N
Yuasa
Isao
I
Saitou
Naruya
N
Clark
Paul F
PF
Boxshall
Geoff
G
Osawa
Motoki
M
Igarashi
Keiji
K
D000818:Animals; D001483:Base Sequence; D003445:Crustacea / Q000145:classification / Q000235:genetics*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000379:methods; D016678:Genome; D005843:Geography; D009144:Museums; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015183:Restriction Mapping; D013045:Species Specificity
D017931:DNA Primers; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S