title: Interleukin-12 inhibits apoptosis in chronic lymphatic leukemia (CLL) B cells.
abstract: The purpose of the present study was to investigate the effect of interleukin-12 on apoptosis of chronic lymphatic leukemia (CLL) B cells. Apoptotic indices were determined in highly purified CD5(+) B lymphocytes isolated from peripheral blood of seven patients with histologically confirmed CLL. Interleukin-4 as a known inhibitor of apoptosis was used as control. Quantitative analysis of apoptosis was determined by cell death detection ELISA. Our findings indicate that interleukin-12 inhibits ex vivo apoptosis in a large proportion of B-CLL patients and may be closely involved in the pathogenesis of disease. Therefore, our results may help identify potential new therapeutic targets in this malignancy.
authors.affiliation: Medizinische Hochschule Hannover, Hämatologie und Onkologie, Hannover, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Schuhknecht
authors.forename: S
authors.initials: S
authors.lastname: Duensing
authors.forename: I
authors.initials: I
authors.lastname: Dallmann
authors.forename: J
authors.initials: J
authors.lastname: Grosse
authors.forename: M
authors.initials: M
authors.lastname: Reitz
authors.forename: J
authors.initials: J
authors.lastname: Atzpodien
mesh_terms: D000328:Adult; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D005260:Female; D006801:Humans; D018664:Interleukin-12 / Q000494:pharmacology* / Q000627:therapeutic use; D015847:Interleukin-4; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D008875:Middle Aged
chemical_list: D018664:Interleukin-12; D015847:Interleukin-4