A sulphated fucan from the Laminaria abyssalis inhibits the human T cell lymphotropic virus type 1-induced syncytium formation in HeLa cells.
This work evaluated the effect of a sulphated fucan extracted from the Laminaria abyssalis marine algae on the human T cell lymphotropic virus type 1 (HTLV-1)-induced syncytium formation. The experiments were carried out in HeLa cells cocultured with a HTLV-1-infected T cell line (C91/PL cells) in the presence of the sulphated polysaccharide at concentration below that corresponding to the ED50. The sulphated fucan inhibited almost 100% of the syncytium formation at concentration of 100 microg/mI and was still active (>95%) at a concentration of 25 microg/ml. It was also observed that the best inhibition occurred when the compound was added in the first 2 h of the cell-to-cell contact. This is the first report showing that a purified sulphated polysaccharide, extracted from marine algae, is able to inhibit the cell-to-cell contact essential for the spreading of the HTLV-1.
Romanos
M T V
MT
Departamento de Virologia do Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Rio de Janeiro, Brazil.
Andrada-Serpa
M J
MJ
Mourão
P A S
PA
Yoneshigue-Valentin
Y
Y
Costa
S S
SS
Pereira
M S
MS
Miranda
M M F S
MM
Gonçalves
J L S
JL
Wigg
M D
MD
D002450:Cell Communication / Q000187:drug effects / Q000502:physiology; D016264:Dextran Sulfate / Q000494:pharmacology; D015726:Giant Cells / Q000187:drug effects* / Q000821:virology; D015490:HTLV-I Infections / Q000821:virology; D006367:HeLa Cells; D015368:Human T-lymphotropic virus 1 / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007795:Laminaria / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000821:virology; D013997:Time Factors; D014407:Tumor Cells, Cultured
D011134:Polysaccharides; D016264:Dextran Sulfate; C007789:fucoidan