Genetic organisation of Iris yellow spot virus M RNA: indications for functional homology between the G(C) glycoproteins of tospoviruses and animal-infecting bunyaviruses.
The complete nucleotide sequence (4838 nucleotides) of Iris yellow spot virus (IYSV) M RNA indicates, typical for tospoviruses, the presence of two genes in ambisense arrangement. The vRNA ORF codes for the potential cell-to-cell movement (NSm) protein (34.8 kDa) and the vcRNA ORF for the viral glycoprotein (G1/G2) precursor (128.6 kDa). Multiple sequence alignment of the NSm and G1/G2 precursor proteins of IYSV with those of other tospoviruses, showed highest homologies to Peanut bud necrosis virus (PBNV) and Watermelon silver mottle virus (WSMV). The potential cell-to-cell movement protein of tospoviruses is highly conserved (40-70% identity), with the exception of the first 60 N terminal amino acids, a domain that clearly diverged. For the G1 and G2 viral glycoproteins, blast searches revealed a significant homology between the C-terminally located tospoviral G1 (G(C)) protein with the counterpart of the animal-infecting bunyaviruses, suggesting a functional homology for these proteins.
Cortez
I
I
Departamento de Protecção de Plantas, ICETA - UTAD, Vila Real, Portugal.
Aires
A
A
Pereira
A-M
AM
Goldbach
R
R
Peters
D
D
Kormelink
R
R
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D006023:Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D053830:Plant Viral Movement Proteins; D010944:Plants / Q000821:virology*; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000737:chemistry; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics*; D016415:Sequence Alignment; D013045:Species Specificity; D017865:Tospovirus / Q000145:classification / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D006023:Glycoproteins; D053830:Plant Viral Movement Proteins; D011498:Protein Precursors; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins