Affects of different access routes on autologous satellite cell implantation stimulating myocardial regeneration.
OBJECTIVE
To study the effect of different access routes on autologous satellite cell implantation to stimulate myocardial regeneration.


METHODS
Satellite cells were procured from skeletal muscle (gluteus max) of adult mongrel canine, cultured, proliferated and labeled with 4', 6-diamidino-2-phenylindone (DAPI) in vitro. The cells were autologously implanted into the site of acute myocardial infarction by local injection or perfusion through the ligated distal left anterior descending coronary artery. Specimens were harvested 2, 4 and 8 weeks later for histological study.


RESULTS
The labeling efficiency of satellite cells with DAPI was close to 100%. Fluorescent cells were found at the infarcted zone, papillary muscle and local injection site. Some of these cells had progressively differentiated into striated muscle fibers connected to intercalated discs. The infant cells appeared different from the mature myocardium under an electron microscope. Satellite cells implanted by perfusion through the coronary artery were arranged in order of consistency with host myocardial fibers. The satellite cells, implanted by local injection, were found growing in a disordered way.


CONCLUSIONS
Satellite cells, implanted by coronary artery perfusion, can progressively differentiate into striated muscle fibers, arranging in order and disseminating over the infarcted zone. This approach seems more favorable for the recovery of myocardial contractile function than that of local injection.
Zhong
Hong
H
Department of Thoracic Surgery, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China. zhonghongyy@21cn.com
Zhu
Hongsheng
H
Zhang
Zhen
Z
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D004285:Dogs; D009203:Myocardial Infarction / Q000473:pathology / Q000628:therapy*; D009206:Myocardium / Q000166:cytology*; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000637:transplantation*; D014182:Transplantation, Autologous