Purification and characterization of a glycoprotein inhibitor of toxic phospholipase from Withania somnifera.
A phospholipase inhibitor (WSG) has been purified from Withania somnifera using gel-filtration and ion-exchange chromatographies. The WSG is an acidic glycoprotein. Its molecular mass as determined by SDS-PAGE was 27kDa. It neutralized the enzyme activity and pharmacological properties such as cytotoxicity, edema, and myotoxicity of a multi-toxic Indian cobra venom phospholipase (NNXIa-PLA) but failed to neutralize the neurotoxicity. The glycan part of the molecule does not appear to be involved in any of the pharmacological properties studied. The results suggest that the neutralization of the pharmacological effects of the toxic phospholipase is brought about by inhibition of the enzyme activity by formation of a complex between the WSG and the toxic phospholipase. We report the purification and characterization of a glycoprotein phospholipase A inhibitor from Withania somnifera, medicinal plant.
Deepa
M
M
Department of Studies in Biochemistry, University of Mysore, Manasagangothri, 570 006, Mysore, India.
Veerabasappa Gowda
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T
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D002470:Cell Survival / Q000187:drug effects; D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D004487:Edema; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D051379:Mice; D018482:Muscle, Skeletal / Q000187:drug effects / Q000473:pathology; D009336:Necrosis; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D018517:Plant Roots / Q000737:chemistry; D032341:Withania / Q000737:chemistry*
D004791:Enzyme Inhibitors; D006023:Glycoproteins; D010741:Phospholipases A