Activity of the A3 adenosine receptor gene promoter in transgenic mice: characterization of previously unidentified sites of expression.
Sites of A3 adenosine receptor gene expression have not been fully explored nor has this gene's promoter activity been confirmed in vivo. Transgenic mice were generated in which 2.3 kb upstream of the transcriptional start site of the mouse A3 adenosine receptor was coupled to a beta-galactosidase reporter gene. Selective transgene expression was detected in testis and brain as well as at other sites in which A3 adenosine receptor message has not been previously reported, including retinal ganglion cells and smooth muscle cells of the cerebrospinal vasculature. Our study suggests that this promoter may be useful in the selective targeting of gene expression to specific tissues.
Yaar
Ron
R
Department of Biochemistry and Whitaker Cardiovascular Institute, Boston University School of Medicine, K225, 715 Albany Street, Boston, MA 02118, USA.
Lamperti
Edward D
ED
Toselli
Paul A
PA
Ravid
Katya
K
D000818:Animals; D001921:Brain / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D007763:Lac Operon; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008957:Models, Genetic; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D051381:Rats; D017207:Rats, Sprague-Dawley; D043684:Receptor, Adenosine A3; D018047:Receptors, Purinergic P1 / Q000235:genetics*; D012160:Retina / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000378:metabolism; D019076:Transgenes; D001616:beta-Galactosidase / Q000378:metabolism
D043684:Receptor, Adenosine A3; D018047:Receptors, Purinergic P1; D001616:beta-Galactosidase