title: Study of urinary nucleosides as biological marker in cancer patients analyzed by micellar electrokinetic capillary chromatography.
abstract: Thirteen normal and modified nucleosides, primarily degradation products of transfer ribonucleic acid (tRNA), were evaluated as potential tumor markers for cancer patients. Their urinary concentrations were determined by means of micellar electrokinetic capillary chromatography (MEKC) in the urine from 54 healthy adults and 70 cancer patients, then quantitatively expressed as a function of creatinine excretion. It was found that urinary nucleosides for cancer patients were on the average significantly higher than those for healthy controls, however, no significant differences were found between male and female or between different ages. Based on 13 urinary nucleoside concentrations, principal component analysis (PCA) could be used to classify 72% of cancer patients from the healthy controls. The present study shows that the precise measurement of urinary nucleosides by MEKC in combining with PCA technique may provide a clinically useful approach for diagnosis of cancer.
authors.affiliation: National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences.
authors.forename: Yu-Fang
authors.initials: YF
authors.lastname: Zheng
authors.forename: Guo-Wang
authors.initials: GW
authors.lastname: Xu
authors.forename: Da-Yu
authors.initials: DY
authors.lastname: Liu
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Xiong
authors.forename: Pu-Dun
authors.initials: PD
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: Shen
authors.initials: S
authors.lastname: Lv
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000652:urine; D002021:Buffers; D002681:China; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D008823:Micelles; D008875:Middle Aged; D009369:Neoplasms / Q000652:urine*; D009705:Nucleosides / Q000652:urine*; D012016:Reference Values; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D002021:Buffers; D007202:Indicators and Reagents; D008823:Micelles; D009705:Nucleosides