[Expression and significance of P-glycoprotein, glutathione S-transferase-pi and Topoisomerase II in gastric carcinomas].
OBJECTIVE
Multi-drug resistance(MDR) is the most important failing reason of tumor chemotherapy, the substance that P-glycoprotein(P-gp), glutathione S-transferase-pi(GST-pi), Topoisomerase II (Topo II) etc was the fundamental substances producing MDR. Gastric carcinoma is the relatively familiar malignancy, the report was relatively fewness that the expression of P-gp, GST-pi and Topo II by means of immunohistochemistry were associated with in the gastric carcinomas of previously untreated patients. This study was designed to investigate the expression and significance of P-gp, glutathione S-transferase-pi(GST-pi) and Topoisomerase II(Topo II) in gastric carcinomas.


METHODS
Seventy-five human gastric carcinomas of previously untreated patients were studied for the expression of P-gp, GST-pi and Topo II by means of immunohistochemistry.


RESULTS
The expression of P-gp, GST-pi and Topo II were 76.0%, 85.3% and 68.0% in gastric carcinoma tissues. There was no significant relationship between the expression levels and age, sex, location of tumor and depth of tumor invasion. Positive rate was higher in glandular-form carcinoma than that in mucoid carcinoma, signet-ring cell carcinoma and poorly differentiated carcinoma (P < 0.05). There was a significant relationship between the lymph node involvement and the higher expression of P-gp, GST-pi, lower expression of Topo II.


CONCLUSIONS
There is a correlation between the expression of P-gp, GST-pi, Topo II and histologic subtypes, the expression may also related to the differentiation and metastasis of the gastric carcinomas.
Qin
Fang
F
Department of Pathology, Jinan Military General Hospital, Jinan 250031, P. R. China. qin fang-f@sohu.com
Qin
Xia
X
Zhang
Xia
X
Jia
Hai-wei
HW
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis*; D000328:Adult; D000368:Aged; D004250:DNA Topoisomerases, Type II / Q000032:analysis*; D005260:Female; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000473:pathology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D007527:Isoenzymes; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D004250:DNA Topoisomerases, Type II