title: Simultaneous determination of metronidazole and miconazole in pharmaceutical dosage forms by RP-HPLC.
abstract: A reversed-phase high performance liquid chromatography (RP-HPLC) method with UV detection is described for the simultaneous determination of metronidazole and miconazole in pharmaceutical dosage forms. Chromatography was carried out on a C18 reversed-phase column, using a mixture of methanol-water (40+60, v/v) as a mobile phase, at a flow rate of 1.0 ml min(-1). Sulfamethoxazole was used as an internal standard and detection was performed using a diode array detector at 254 nm. The method produced linear responses in the concentration ranges 10-70 and 1-20 microg ml(-1) with detection limits 0.33 and 0.27 microg ml(-1) for metronidazole and micanozole, respectively. This procedure was found to be convenient and reproducible for analysis of these drugs in ovule dosage forms.
authors.affiliation: Department of Pharmaceutical Sciences, Gülhane Military Medical Academy, 06018 Ankara, Turkey.
authors.forename: Cemal
authors.initials: C
authors.lastname: Akay
authors.forename: Sibel A
authors.initials: SA
authors.lastname: Ozkan
authors.forename: Zühre
authors.initials: Z
authors.lastname: Sentürk
authors.forename: Semsettin
authors.initials: S
authors.lastname: Cevheroğlu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004304:Dosage Forms; D008795:Metronidazole / Q000032:analysis*; D008825:Miconazole / Q000032:analysis*; D012015:Reference Standards; D012044:Regression Analysis; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004304:Dosage Forms; D008795:Metronidazole; D008825:Miconazole