title: Solution structure of fatty acid-binding protein from human brain.
abstract: Human brain-type fatty acid-binding protein (B-FABP) has been recombinantly expressed in Escherichia coli both unlabelled and 15N-enriched for structure investigation in solution using high-resolution NMR spectroscopy. The sequential assignments of the 1H and 15N resonances were achieved by applying multidimensional homo- and heteronuclear NMR experiments. The ensemble of the 20 final energy-minimized structures, representing human B-FABP in solution, have been calculated based on a total of 2490 meaningful distance constraints. The overall B-FABP structure exhibits the typical backbone conformation described for other members of the FABP family, consisting often antiparallel beta-strands (betaA to betaJ) that form two almost orthogonal beta-sheets, a helix-turn-helix motif that closes the beta-barrel on one side, and a short N-terminal helical loop. A comparison with the crystal structure of the same protein complexed with docosahexaenoic acid reveals only minor differences in both secondary structure and overall topology. Moreover, the NMR data indicate a close structural relationship between human B-FABP and heart-type FABP with respect to fatty acid binding inside the protein cavity.
authors.affiliation: Institut für Biophysikalische Chemie, Johann Wolfgang Goethe-Universität, Frankfurt a. M., Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Rademacher
authors.forename: Aukje W
authors.initials: AW
authors.lastname: Zimmerman
authors.forename: Heinz
authors.initials: H
authors.lastname: Rüterjans
authors.forename: Jacques H
authors.initials: JH
authors.lastname: Veerkamp
authors.forename: Christian
authors.initials: C
authors.lastname: Lücke
mesh_terms: D000595:Amino Acid Sequence; D001923:Brain Chemistry; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000074026:Fatty Acid Binding Protein 3; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D019077:Helix-Turn-Helix Motifs; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012996:Solutions; D025521:Tumor Suppressor Proteins
chemical_list: D002352:Carrier Proteins; C493408:FABP3 protein, human; C107608:FABP7 protein, human; D000074026:Fatty Acid Binding Protein 3; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; D012996:Solutions; D025521:Tumor Suppressor Proteins