Identification of neuropeptide FF-related peptides in human cerebrospinal fluid by mass spectrometry.
Several neuropeptide FF (NPFF)-related peptides, known as modulators of the opioid system, have been previously characterized in bovine and rodent brain. Reverse-phase high pressure liquid chromatography (HPLC) fractions of a human with normal pressure hydrocephalus cerebrospinal fluid (CSF), co-migrating with NPFF-related synthetic peptides, were characterized by capillary HPLC coupled on-line to nanospray ion trap tandem mass spectrometry. Two peptides present in the pro-NPFF(A) precursor, NPAF (AGEGLNSQFWSLAAPQRF-NH2) and NPSF (SLAAPQRF-NH2), were identified. The monitoring of NPFF-related peptides in human CSF can be helpful to understand their roles in pain sensitivity.
Burlet-Schiltz
Odile
O
Institut de Pharmacologie et de Biologie Structurale, CNRS UMR 5089, 205 route de Narbonne, 31077, Toulouse, France.
Mazarguil
Honoré
H
Sol
Jean-Christophe
JC
Chaynes
Patrick
P
Monsarrat
Bernard
B
Zajac
Jean-Marie
JM
Roussin
Anne
A
D000595:Amino Acid Sequence; D002555:Cerebrospinal Fluid / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000134:cerebrospinal fluid / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011863:Radioimmunoassay; D013997:Time Factors
D009842:Oligopeptides; D010455:Peptides; C053651:phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide