title: Undersulfation of proteoglycans and proteins alter C6 glioma cells proliferation, adhesion and extracellular matrix organization.
abstract: Proteoglycans are considered to be important molecule in cell-microenvironment interactions. They are overexpressed in neoplastic cells modifying their growth and migration in hosts. In this work we verified that undersulfation of proteoglycans and other sulfated molecules, induced by sodium chlorate treatment, inhibited C6 glioma cells proliferation in a dose-dependent way. This effect was restored by the addition of exogenous heparin. We could not detect significant cell mortality in our culture condition. The treatment also impaired in a dose-dependent manner, C6 cell adhesion to extracellular matrix (ECM) proteins (collagen IV, laminin and fibronectin). In addition, sodium chlorate treatment altered C6 glioma cell morphology, from the fibroblast-like to a more rounded one. This effect was accompanied by increased synthesis of fibronectin and alterations in its extracellular network organization. However, we could not observe modifications on laminin organization and synthesis. The results suggest an important connection between sulfation degree with important tumor functions, such as proliferation and adhesion. We suggest that proteoglycans may modulate the glioma microenvironment network during tumor cell progression and invasion.
authors.affiliation: Department Biologia Celular, Embriologia e Genética, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário-Trindade, 88040-900 Florianópolis, S.C., Brazil.
authors.forename: Claudia B N
authors.initials: CB
authors.lastname: Mendes de Aguiar
authors.forename: Ricardo Castilho
authors.initials: RC
authors.lastname: Garcez
authors.forename: Marcio
authors.initials: M
authors.lastname: Alvarez-Silva
authors.forename: Andréa Gonçalves
authors.initials: AG
authors.lastname: Trentin
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002704:Chlorates / Q000008:administration & dosage / Q000378:metabolism*; D005109:Extracellular Matrix / Q000187:drug effects; D005353:Fibronectins / Q000648:ultrastructure; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology*; D005910:Glioma / Q000378:metabolism / Q000473:pathology; D007797:Laminin / Q000648:ultrastructure; D011506:Proteins / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D012016:Reference Values; D013431:Sulfates / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D002704:Chlorates; D005353:Fibronectins; D007797:Laminin; D011506:Proteins; D011509:Proteoglycans; D013431:Sulfates; C032706:sodium chlorate