[Establishment of a cisplatin-induced human hepatocellular carcinoma drug-resistant cell line and its biological characteristics].
OBJECTIVE
Drug resistance is a popular topic in tumor study. The drug-resistant cell line established in vitro is generally used as the research model. To explore the mechanism of resistance of hepatocellular carcinoma(HCC) to cisplatin, we designed this study to establish a cisplatin-induced human hepatocellular carcinoma drug-resistant cell line and study its characteristics.


METHODS
A resistant HCC cell line (QGY/cDDP) was established gradually by increasing dose of cisplatin and intermittent administration; drug sensitivity was detected by MTT assay; the changes of its biological characteristics were determined using light microscopy, electron microscopy, cell counting, flow cytometry(FCM), and chromatosome analysis.


RESULTS
QGY/cDDP cell line was developed after 3 months with stable resistance to cisplatin and the resistance index was 10.81; QGY/cDDP cells exhibited cross-resistance to many other chemotherapeutic agents (5-Fuorouracil, epiadriamycin, etc). The morphology and chromatosome number of QGY/cDDP changed; doubling time prolonged; and the cell number of S-phase and G2/M-phase decreased while in G0/G1 phase increased compared with parent cells.


CONCLUSIONS
QGY/cDDP cell line shows the typical and stable resistant phenotype.
Yang
Jun-xia
JX
Department of Pharmacology, Chongqing Medical University, Chongqing 400016, P. R. China. junxia-yang@263.net
Tang
Wei-xue
WX
D000970:Antineoplastic Agents / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002869:Chromosome Aberrations; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D005434:Flow Cytometry; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D002945:Cisplatin