title: [An experimental study on c-myc gene mRNA expression of endothelial cell induced by bFGF].
abstract: OBJECTIVE
  To investigate the molecular biological mechanism of endothelial cell proliferation induced by bFGF.
  
  
  METHODS
  Cultured rat myocardial microvascular endothelial cells were stimulated with bFGF of various concentrations. By northern blot analysis, the levels of c-myc mRNA expression were detected.
  
  
  RESULTS
  The expression of c-myc mRNA in the bFGF-treated groups increased (P < 0.05) with a dose- and stimulating time-dependent manner. The c-myc mRNA expression reached to a peak level at 2 hours.
  
  
  CONCLUSIONS
  C-myc expression may be an important component in controlling the transit of cells through the cell cycle.
authors.affiliation: Department of Plastic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhou
authors.forename: Qinglian
authors.initials: Q
authors.lastname: He
authors.forename: Zihao
authors.initials: Z
authors.lastname: Lin
authors.forename: Songlin
authors.initials: S
authors.lastname: Yang
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D016259:Genes, myc / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2