Interleukin-1-induced glenohumeral synovitis and shoulder pain in rotator cuff diseases.
Synovitis of the subacromial bursa has been identified as a main source of shoulder pain in rotator cuff diseases. Little interest, however, has been paid into the synovitis of glenohumeral joint. The mRNA expression levels of interleukin-1beta (IL-1beta) and interleukin-1 receptor antagonists produced in the synovitis reflect the magnitude of inflammation. The present study was undertaken to determine the relationship between mRNA expression levels of IL-1beta and its receptor antagonists (secreted interleukin-1 receptor antagonist (IL-1ra) and intracellular IL-1ra) in the synovium of the glenohumeral joint and shoulder pain in rotator cuff diseases, analyzing the synovial specimens by reverse transcriptase polymerase chain reaction. Thirty-five patients with rotator cuff diseases were candidates. Based on the presence of cuff perforation, they were divided into two categories: 16 with non-perforating tears and 19 with perforating tears. The degree of shoulder pain was evaluated by use of a visual analogue scale. The pain degree of non-perforating tears was significantly greater than that of perforating tears (P < 0.01). In contrast, the expression levels of the cytokine-mRNAs were constitutively greater in perforating tears than in non-perforating tears (P < 0.01, respectively). The expression levels of the cytokine-mRNAs were inversely correlated with the degree of pain (IL-1beta: r = 0.930; secreted IL-1ra: r = 0.861; intracellular IL-1ra: r = 0.932, P < 0.001 respectively). These results suggest that the expression levels of the cytokine-mRNAs in the synovium of the glenohumeral joint contribute less to the generation of shoulder pain in rotator cuff diseases.
Gotoh
Masafumi
M
Department of Orthopaedic Surgery, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan. gotomasa@kurume.ktarn.or.jp
Hamada
Kazutoshi
K
Yamakawa
Hideyuki
H
Yanagisawa
Kazuhiro
K
Nakamura
Masato
M
Yamazaki
Hitoshi
H
Inoue
Akio
A
Fukuda
Hiroaki
H
D000328:Adult; D000368:Aged; D005260:Female; D015870:Gene Expression; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010146:Pain / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D012333:RNA, Messenger / Q000032:analysis; D017006:Rotator Cuff / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D012785:Shoulder Joint / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D013585:Synovitis / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*
D007375:Interleukin-1; D012333:RNA, Messenger