title: A novel solid support for synthesis of oligonucleotide 3'-phosphorothioate monoesters.
abstract: A new reagent immobilized on solid support allowing for solid-phase synthesis of oligonucleotides with a 3'-terminal phosphorothioate monoester is described. The support is compatible with phosphoramidite chemistry for automated oligonucleotide synthesis. Final deprotection with ammonia under standard conditions leads to oligonucleotide 3'-terminal phosphorothioate.
authors.affiliation: Isis Pharmaceuticals, 2292 Faraday Avenue, Carlsbad, CA 92008, USA.
authors.forename: Zacharia S
authors.initials: ZS
authors.lastname: Cheruvallath
authors.forename: Douglas L
authors.initials: DL
authors.lastname: Cole
authors.forename: Vasulinga T
authors.initials: VT
authors.lastname: Ravikumar
mesh_terms: D000641:Ammonia / Q000737:chemistry; D019075:Electrophoresis, Capillary; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009946:Organothiophosphorus Compounds / Q000138:chemical synthesis*
chemical_list: D007202:Indicators and Reagents; D009841:Oligonucleotides; D009946:Organothiophosphorus Compounds; D000641:Ammonia