title: A "step down" PCR-based technique for walking into and the subsequent direct-sequence analysis of flanking genomic DNA.
authors.affiliation: Department of Plant Sciences, University of Oxford, Oxford, UK.
authors.forename: Ziguo
authors.initials: Z
authors.lastname: Zhang
authors.forename: Sarah Jane
authors.initials: SJ
authors.lastname: Gurr
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D017931:DNA Primers; D007202:Indicators and Reagents; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping / Q000379:methods; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D017931:DNA Primers; D007202:Indicators and Reagents; D004247:DNA