title: Identification of a FasL-like molecule in leucocytes of the teleost fish gilthead seabream (Sparus aurata L.).
abstract: The possible expression of FasL in gilthead seabream leucocytes was studied by flow cytometry, immunoblotting and immunocytochemistry, using an anti-mouse FasL monoclonal antibody. The results pointed to a cytosolic FasL-like, but not a membrane-like form, in resting leucocytes from head-kidney, thymus, spleen, blood and peritoneal exudate. Immunoblotting revealed a 19kDa band in resting leucocytes, while activated leucocytes showed the same band and another of 39kDa. The FasL-like molecule is identified in lymphocytes, monocyte-macrophages and acidophilic granulocytes. Phylogenetical and functional implications are suggested.
authors.affiliation: Department of Cell Biology, Faculty of Biology, University of Murcia, Campus Universitario de Espinardo, 30100 Murcia, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Cuesta
authors.forename: M Angeles
authors.initials: MA
authors.lastname: Esteban
authors.forename: José
authors.initials: J
authors.lastname: Meseguer
mesh_terms: D001202:Ascitic Fluid / Q000276:immunology; D053222:Fas Ligand Protein; D005434:Flow Cytometry; D015151:Immunoblotting; D007150:Immunohistochemistry; D007668:Kidney / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000737:chemistry; D008970:Molecular Weight; D021541:Sea Bream / Q000097:blood / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D019014:fas Receptor / Q000032:analysis*
chemical_list: D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008562:Membrane Glycoproteins; D019014:fas Receptor