title: Decreased frequency of intracellular IFN-gamma producing T cells in whole blood preparations from patients with atopic dermatitis.
abstract: There have been contradictory reports on the shift in the T-cell cytokine expression pattern of peripheral blood mononuclear cells from patients with atopic dermatitis (AD); more specifically the interleukin (IL)-4 and interferon (IFN)-gamma profiles. The aim of this study was to shed further light on this contradiction by measuring the intracellular cytokines IL-4 and IFN-gamma by flow cytometry on unseparated whole blood to obtain results that, as accurately as possible, reflect the situation in circulating cells in vivo. The patient group including 64 patients with AD was compared with 18 nonatopic healthy adults. The results showed that the percentage of CD4+ T cells expressing IFN-gamma was significantly decreased (P < or = 0.001), as well as the percentage expressing IL-4 (P < 0.05) in AD patients compared with healthy controls. Furthermore, in supernatants from whole blood samples stimulated with phorbol 12-myristate 13-acetate and ionomycin, production of IFN-gamma was significantly decreased, while IL-4 production remained unchanged in AD patients compared with healthy controls. We also investigated if there was a relationship between serum IgE level and Phadiatop, a screening test for atopy, vs. the levels of IL-4 and IFN-gamma, but found no correlation with either. However, there was a significant correlation between disease severity and the level of total IgE (r = 0.67, P < 0.05). In conclusion, our results support the evidence for a decreased ability of peripheral CD4+ T cells to produce IFN-gamma among AD patients.
authors.affiliation: Department of Medicine, Division of Clinical Immunology and Allergy, Karolinska Institutet and Hospital, Stockholm, Sweden.
authors.forename: Eva
authors.initials: E
authors.lastname: Källström
authors.forename: Ingrid
authors.initials: I
authors.lastname: Roscher
authors.forename: Annica
authors.initials: A
authors.lastname: Andreasson
authors.forename: Ove
authors.initials: O
authors.lastname: Bäck
authors.forename: Marianne
authors.initials: M
authors.lastname: van Hage-Hamsten
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017252:CD3 Complex / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D016022:Case-Control Studies; D016207:Cytokines / Q000096:biosynthesis; D003876:Dermatitis, Atopic / Q000097:blood* / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D012720:Severity of Illness Index
chemical_list: D017252:CD3 Complex; D016207:Cytokines; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma