title: Monitoring of active HHV-6 infection in bone marrow transplant recipients by real time PCR; comparison to detection of viral DNA in plasma by qualitative PCR.
abstract: Twelve (46%) of the 26 patients had human herpesvirus 6 (HHV-6) viremia after bone marrow transplant (BMT). All isolates were recovered from the samples obtained at 2 weeks after BMT. The sensitivity and the specificity of detection of viral DNA in plasma by qualitative polymerase chain reaction (PCR) for monitoring active virus replication were 92% and 97% respectively. Moreover, the positive (85%) and negative (99%) predictive values were also high. The patients with HHV-6 viremia showed a clear peak in HHV-6 DNA in peripheral blood mononuclear cells (PBMCs) at 2 weeks after BMT, which was measured by real time PCR. The virus DNA level in PBMCs between the two groups (patients with viremia and patients without viremia) was statistically different at 2 weeks after BMT (P = 0.033). In patients with HHV-6 viremia, mean HHV-6 DNA copy number was higher in the samples collected at 2 weeks after BMT than the samples collected at any other time period.
authors.affiliation: Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Ihira
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Kyoko
authors.initials: K
authors.lastname: Suzuki
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ohashi
authors.forename: Sadao
authors.initials: S
authors.lastname: Suga
authors.forename: Keizo
authors.initials: K
authors.lastname: Horibe
authors.forename: Naoko
authors.initials: N
authors.lastname: Tanaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kimura
authors.forename: Seiji
authors.initials: S
authors.lastname: Kojima
authors.forename: Koji
authors.initials: K
authors.lastname: Kato
authors.forename: Takaharu
authors.initials: T
authors.lastname: Matsuyama
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Nishiyama
authors.forename: Yoshizo
authors.initials: Y
authors.lastname: Asano
mesh_terms: D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D004279:DNA, Viral / Q000097:blood* / Q000737:chemistry / Q000235:genetics; D005260:Female; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016867:Immunocompromised Host; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D019349:Roseolovirus Infections / Q000097:blood / Q000821:virology*; D012680:Sensitivity and Specificity; D014766:Viremia / Q000175:diagnosis
chemical_list: D004279:DNA, Viral