Inhibitory effect of p27KIP1 gene transfer on head and neck squamous cell carcinoma cell lines.
BACKGROUND
Although p27 gene mutations are rarely found in cancer, the level of p27 protein expression decreases during tumor development. In several tumors, including laryngeal cancer, decreased expression of p27 is associated with a poor prognosis.


METHODS
The proliferation-inhibitory effect of p27 gene transfer on human head and neck squamous cell carcinoma (HNSCC) cell lines (SNU-1041, SNU-1066, and SNU-1076) by adenoviral vector was investigated.


RESULTS
On transduction of the human HNSCC cell line with adenovirus-p27 (Ad-p27), a high level of p27 expression and increase of cyclin D1 and E were observed. Cell cycle analysis showed a marked decrease in the proportion of cells in S phase and an increase in G1 phase. Soft agar clonogenic assay showed a marked decrease in clonogenicity.


CONCLUSIONS
These results suggested that overexpression of p27 could show proliferation-inhibitory effects on HNSCC cell lines. Thus, gene therapy using adenovirus-p27 seemed to have a potential to develop into a new cancer gene therapy modality.
Koh
Tae-Yong
TY
Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul, 110-744, Korea.
Park
Seok-Woo
SW
Park
Kyung-Ho
KH
Lee
Sang-Goo
SG
Seol
Jae-Goo
JG
Lee
Dong-Wook
DW
Lee
Choon-Taek
CT
Heo
Dae-Seog
DS
Kim
Kwang-Hyun
KH
Sung
Myung-Whun
MW
D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D017209:Apoptosis; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000628:therapy*; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D015316:Genetic Therapy; D005822:Genetic Vectors; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000628:therapy*; D006801:Humans; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
D018797:Cell Cycle Proteins; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases