alpha5 Subunit-containing GABA(A) receptors form clusters at GABAergic synapses in hippocampal cultures.
We have used triple-label fluorescence immunocytochemistry to demonstrate that alpha5 subunit-containing GABA(A) receptors (GABA(A)Rs) form large clusters at GABAergic synapses in dendrites and axon initial segment of cultured hippocampal neurons. The large synaptic clusters of alpha5 subunit-containing GABA(A)Rs also contained alpha1, beta2/3, gamma2 GABA(A)R subunits and gephyrin. The alpha5 subunit-containing GABA(A)Rs also formed small clusters. The small clusters were not associated with GABAergic synapses and often did not co-localize with gephyrin.
Christie
Sean B
SB
Department of Physiology and Neurobiology, University of Connecticut, Storrs, CT 06268, USA.
de Blas
Angel L
AL
D000818:Animals; D001369:Axons / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D005333:Fetus; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000378:metabolism; D009433:Neural Inhibition / Q000502:physiology*; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D021122:Protein Subunits / Q000378:metabolism*; D017966:Pyramidal Cells / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism* / Q000648:ultrastructure; D009435:Synaptic Transmission / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D002352:Carrier Proteins; D008565:Membrane Proteins; D021122:Protein Subunits; D011963:Receptors, GABA-A; C073441:gephyrin; D005680:gamma-Aminobutyric Acid