title: Analysis of chromosome conservation in Lemur catta studied by chromosome paints and BAC/PAC probes.
abstract: A panel of human chromosome painting probes and bacterial and P1 artificial chromosome (BAC/PAC) clones were used in fluorescence in situ hybridization (FISH) experiments to investigate the chromosome conservation of the ring-tailed lemur (Lemur catta, LCA) with respect to human. Whole chromosome paints specific for human chromosomes 7, 9, 11, 13, 14, 17, 18, 20, 21, and X were found to identify a single chromosome or an uninterrupted chromosomal region in LCA. A large set of partial chromosome paints and BAC/PAC probes were then used to refine the characterization of the rearrangements differentiating the two karyotypes. The results were also used to reconstruct the ancestral Lemuridae karyotype. Lemur catta, indeed, can be used as an outgroup, allowing symplesiomorphic (ancestral) rearrangements to be distinguished from apomorphic (derived) rearrangements in lemurs. Some LCA chromosomes are difficult to distinguish morphologically. The 'anchorage' of most LCA chromosomes to specific probes will contribute to the standardization of the karyotype of this species.
authors.affiliation: Sezione di Genetica, DAPEG, Via Amendola 165/A, 70126 Bari, Italy.
authors.forename: Maria Francesca
authors.initials: MF
authors.lastname: Cardone
authors.forename: Mario
authors.initials: M
authors.lastname: Ventura
authors.forename: Sergio
authors.initials: S
authors.lastname: Tempesta
authors.forename: Mariano
authors.initials: M
authors.lastname: Rocchi
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Archidiacono
mesh_terms: D000818:Animals; D005075:Biological Evolution; D020223:Chromosome Painting; D002875:Chromosomes / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial; D027042:Chromosomes, Artificial, P1 Bacteriophage; D007621:Karyotyping; D007900:Lemur / Q000235:genetics*; D015341:Nucleic Acid Probes; D026801:Synteny / Q000235:genetics*
chemical_list: D015341:Nucleic Acid Probes