Differential expression of a 70 kDa O-glycoprotein on T cells: a possible marker for naive and early activated murine T cells.
We purified a 70 kDa O-glycoprotein that binds to the GalNAc specific lectin from Amaranthus leucocarpus (ALLr) and determined its expression pattern on T lymphocytes from different murine lymphoid organs. High level of ALLr expression was demonstrated in 95-98% of both CD4(+)8(+) and CD4(-)8(+) thymocytes, and in 80-95% of CD8(+) T cells from peripheral blood, lymph nodes, and spleen, whereas a minor fraction of CD4(+)8(-) thymocytes (46-67%) and peripheral CD4(+) T cells (9-40%) showed low ALLr expression. Peripheral CD19(+) B cells were ALLr negative and most of the peripheral ALL(+) T cells showed a CD62L(hi)CD45RB(hi)CD44(lo/-) phenotype, indicating features of naive cells. Mitogenic activation of peripheral T cells increased 3-fold the number of ALL(+)CD4(+) T cells 24 h after stimulation, as opposed to a >80% decrease in CD8(+) T cells 72 h after stimulation. Our results suggest that ALL detects a non-described surface O-glycoprotein selectively expressed by naive CD8(+) T cells and by early activated CD4(+) T cells.
Ortíz
Blanca
B
Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, Calzada de Tlalpan 4502, 14080 Mexico City, Mexico.
Porras
Flor
F
Jiménez-Martínez
M C
MC
Montaño
Luis Felipe
LF
Martínez-Cairo
Salvador
S
Lascurain
Ricardo
R
Zenteno
Edgar
E
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D002846:Chromatography, Affinity; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D008297:Male; D008562:Membrane Glycoproteins / Q000302:isolation & purification* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D037121:Plant Lectins / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011975:Receptors, Mitogen / Q000302:isolation & purification* / Q000378:metabolism; D012794:Sialic Acids / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000737:chemistry* / Q000378:metabolism
C121359:Amaranthus leucocarpus lectin; C470691:Amaranthus leucocarpus lectin receptor, mouse; D015703:Antigens, CD; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D037121:Plant Lectins; D011975:Receptors, Mitogen; D012794:Sialic Acids