title: Assessment of performance of laboratories in determining acrylamide in crispbread.
abstract: A proficiency testing round was undertaken to assess the performance of laboratories to measure acrylamide in a sample of crispbread. Retail samples of crispbread were ground to a fine powder and after thorough mixing were packed in 40 g units for distribution. Ten samples were selected at random and analyzed in duplicate for acrylamide by liquid chromatography/mass spectrometry (LC/MS). Standard statistical tests showed that the material was homogeneous for the purposes of proficiency testing. Test samples were distributed to 55 laboratories in 16 countries in Europe, North America, Australia, and the Middle East. The results were analyzed by standard proficiency testing statistical procedures, and laboratories were awarded z-scores on the basis of their reported results. Based on a target standard deviation (sigmap value) taken from the Horwitz equation, for a robust mean value of 1.2 mg/kg acrylamide, satisfactory results (z-score within +/- 2 for those between 0.8 and 1.6 mg/kg) were obtained by 86% of the 37 laboratories that returned results. Only 1 laboratory was unsatisfactory and 4 had questionable results. About equal numbers of laboratories used gas chromatography (GC)/MS and LC/MS procedures with about 25% using MS/MS and one using GC with electron capture detection. There was no evident trend in performance or bias in results. GC/MS and LC/MS data were evenly distributed across the population of laboratories reporting results.
authors.affiliation: Department for Environment Food and Rural Affairs, Central Science Laboratory, Sand Hutton, York, United Kingdom. db.clarke@csl.gov.uk
authors.forename: Don B
authors.initials: DB
authors.lastname: Clarke
authors.forename: Janet
authors.initials: J
authors.lastname: Kelly
authors.forename: Lesley A
authors.initials: LA
authors.lastname: Wilson
mesh_terms: D000178:Acrylamides / Q000032:analysis*; D001939:Bread / Q000032:analysis*; D002853:Chromatography, Liquid; D008401:Gas Chromatography-Mass Spectrometry; D015203:Reproducibility of Results
chemical_list: D000178:Acrylamides