Development and evaluation of a nano-electrospray ionisation source for atmospheric pressure ion mobility spectrometry.
A nano-electrospray ionisation source has been designed and constructed for a high temperature ion mobility spectrometer. The drift cell was modified by replacement of the 63Ni atmospheric pressure chemical ionisation source with a tube lens/desolvation region and operated using commercial nano-electrospray capillaries. Ions were introduced into the drift region via a Bradbury-Nielson gate (pulse width 50 micros, repetition period 20 ms). A unidirectional flow of nitrogen was used as the drift gas at temperatures in the range 100-150 degrees C to aid desolvation. The performance of the nano-electrospray ion source has been demonstrated for analytes including crown ethers, amino acids and peptides. Reduced mobilities determined by nano-ESI were consistent with those reported using a 63Ni ion source.
Bramwell
Claire J
CJ
Department of Chemistry and Physics, Nottingham Trent University, UK.
Colgrave
Michelle L
ML
Creaser
Colin S
CS
Dennis
Richard
R
D000596:Amino Acids / Q000032:analysis; D013058:Mass Spectrometry / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation*; D009842:Oligopeptides / Q000032:analysis; D011506:Proteins / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization
D000596:Amino Acids; D009842:Oligopeptides; D011506:Proteins