Genomic organization of the murine aminomethyltransferase gene (Amt).
Aminomethyltransferase (Amt), also called glycine cleavage system T-protein is an important enzyme in glycine metabolism (EC 2.1.2.10). Mutations in this gene in humans lead to nonketotic hyperglycinemia, a fatal Mendelian disease. Here, we report the cloning and sequencing of the murine Amt gene. The murine Amt gene consists of nine closely spaced exons that are contained within approximately 5 kb of genomic DNA. It encodes a protein of 403 amino acids that is highly homologous to other mammalian aminomethyltransferases. The cis-acting promoter of the Amt gene is likely to be very short as immediately upstream of the murine Amt gene another gene termed Nicolin 1 gene (Nicn1) is located.
Backofen
Bianca
B
Institute of Animal Breeding and Genetics, School of Veterinary Medicine Hannover, Bünteweg 17p, 30559 Hannover, Germany.
Leeb
Tosso
T
D000595:Amino Acid Sequence; D050960:Aminomethyltransferase; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis; D015698:Genomic Library; D019877:Hydroxymethyl and Formyl Transferases / Q000235:genetics*; D020158:Hyperglycinemia, Nonketotic / Q000201:enzymology / Q000235:genetics; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D004247:DNA; D019877:Hydroxymethyl and Formyl Transferases; D050960:Aminomethyltransferase