Bovine colostrum in oral treatment of enterogenic endotoxaemia in rats.
BACKGROUND
Under conditions of shock, bacteria and endotoxins in the intestines can traverse the mucosal barrier by translocation and enter the blood and lymphatic system. Immunoglobulins and lactoferrin have been reported to neutralize endotoxins and bacteria. We studied the essential therapeutic factors of colostrum products in an animal experiment.


METHODS
We simulated endotoxaemia by per-oral administration of a suspension of Escherichia coli and antibiotics into the duodenum of anaesthetized rats after giving intraperitoneal carrageenan. At the same time, pure bovine colostrum or lactoferrin-enriched bovine colostrum was given. Therapeutic effects were studied by examining plasma endotoxin activity and bacterial contamination of mesenterial lymph nodes and peritoneal lavages. Albumin was used in a control group.


RESULTS
The most effective bovine colostrum was able to reduce the maximum plasma endotoxin value by 67% as compared with the albumin group. The combination of this colostrum with lactoferrin brought about a reduction by 80%. The reduction in bacterial contamination of lymph nodes and peritoneal lavages was also evident.


CONCLUSIONS
Both gammaglobulin and lactoferrin may help to eliminate endotoxins when bovine colostrum is administered into the gut in conditions of septic shock.
Döhler
J Rüdiger
JR
Department of Orthopaedic, Trauma and Hand Surgery, Klinikum Plau am See, Mecklenburg-Vorpommern, Germany. doehler@plau.mediclin.de
Nebermann
Lars
L
D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial; D003126:Colostrum / Q000737:chemistry*; D004731:Endotoxins / Q000097:blood; D005260:Female; D007781:Lactoferrin / Q000494:pharmacology*; D008198:Lymph Nodes / Q000382:microbiology; D008297:Male; D011247:Pregnancy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012772:Shock, Septic / Q000628:therapy*; D018709:Statistics, Nonparametric
D004731:Endotoxins; D007781:Lactoferrin