Polyamine-assisted rapid and clean cleavage of oligonucleotides from cis-diol bearing universal support.
Polyamine-assisted deprotection conditions have been developed for the rapid and clean cleavage of oligonucleotide chains from a cis-diol group bearing universal polymer support, making it compatible with modern oligonucleotide synthesis via all types of phosphoramidite synthons, including base labile protecting group bearing synthons as well. The synthesized oligonucleotides were found to be comparable with the corresponding standard oligomers with respect to their retention time on HPLC, mass on MALDI-TOF and biological activity in PCR amplification.
Kumar
P
P
Nucleic Acids Research Laboratory, Institute of Genomics and Integrative Biology (formerly Centre for Biochemical Technology), Mall Road, Delhi University Campus, Delhi 110 007, India.
Dhawan
G
G
Chandra
R
R
Gupta
K C
KC
D000588:Amines / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004896:Erythritol / Q000031:analogs & derivatives; D008670:Metals / Q000494:pharmacology; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011108:Polymers / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013096:Spermine / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D000588:Amines; D008670:Metals; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D011073:Polyamines; D011108:Polymers; C434331:phosphoramidite; D013096:Spermine; D004896:Erythritol