title: Proteomic study of the soluble proteins from the unicellular cyanobacterium Synechocystis sp. PCC6803 using automated matrix-assisted laser desorption/ionization-time of flight peptide mass fingerprinting.
abstract: The unicellular cyanobacteria Synechocystis sp. (PCC6803) has become a model organism for a range of biochemical and molecular biology studies aimed at investigating environmental stress responses. In this study the soluble proteins of Synechocystis were analysed using narrow pH range (pH 4.5-5.5) zoom gels, automated matrix-assisted laser desorption/ionization mass spectrometry acquisition, spectral processing and database searching. The work sets the foundation for investigations of proteomic changes following stress treatment. One hundred and ninety-two protein spots were analysed and 105 proteins identified, of these 37 were novel proteins not previously seen on two-dimensional gels. Proteins involved in amino acid biosynthesis, energy metabolism and protein modification were identified using this fully automated procedure demonstrating that automated acquisition and processing will be a useful tool for proteomic analyses on this organism.
authors.affiliation: School of Biological and Biomedical Sciences, University of Durham, Durham, UK.
authors.forename: William J
authors.initials: WJ
authors.lastname: Simon
authors.forename: John J
authors.initials: JJ
authors.lastname: Hall
authors.forename: Iwane
authors.initials: I
authors.lastname: Suzuki
authors.forename: Norio
authors.initials: N
authors.lastname: Murata
authors.forename: Antoni R
authors.initials: AR
authors.lastname: Slabas
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D000458:Cyanobacteria / Q000737:chemistry*; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D006863:Hydrogen-Ion Concentration; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome