Lac z Histochemistry and immunohistochemistry reveal ephrin-B ligand expression in the inner ear.
Immunostaining in transgenic mice carrying the lac z gene can be used to map gene and protein distribution in a single tissue. In this study, we examined inner ears from ephrin-B3 homozygous and ephrin-B2 heterozygous mice. Ephrin-B3 lac z expression was limited in these mice. However, immunostaining revealed ephrin-B3 throughout cochlear and vestibular regions. Immunoreactivity was absent in ephrin-B3-homozygous null mutants, demonstrating the specificity of the antibody. Ephrin-B2 lac z reactivity was detected in a limited number of cells in cochlear and vestibular regions. Different immunostaining patterns were found with different antibodies. Comparison with lac z expression indicated which antibody was specific for the transmembrane-bound ephrin-B2 ligand.
Bianchi
Lynne M
LM
Neuroscience Program, Oberlin College, Ohio 44074, USA. lynne.bianchi@oberlin.edu
Dinsio
Kyl
K
Davoli
Katherine
K
Gale
Nicholas W
NW
D000818:Animals; D007758:Ear, Inner / Q000196:embryology / Q000378:metabolism*; D036388:Ephrin-B2 / Q000235:genetics / Q000378:metabolism*; D036389:Ephrin-B3 / Q000235:genetics / Q000378:metabolism*; D006651:Histocytochemistry; D008024:Ligands; D051379:Mice; D008817:Mice, Mutant Strains; D009154:Mutation; D001616:beta-Galactosidase / Q000378:metabolism*
D036388:Ephrin-B2; D036389:Ephrin-B3; D008024:Ligands; D001616:beta-Galactosidase