HIV type 1 abrogates TAP-mediated transport of antigenic peptides presented by MHC class I. Transporter associated with antigen presentation.
Downregulation of MHC class I expression following human immunodeficiency virus 1 (HIV-1) infection is thought to play an important role in viral escape from immune recognition by cytotoxic T-lymphocytes (CTLs). Since exogenous addition of HIV-1-derived peptides restores susceptibility of HIV-1-infected cells to CTL-mediated lysis, we tested whether endogenous peptide loading is impaired in these cells. Our results show that in HIV-1-infected cells the ability of the transporter associated with antigen presentation (TAP) to translocate antigenic peptides from the cytosol to the lumen of the ER for presentation on MHC class I molecules is abolished. These data suggest that interference with the supply of antigenic peptides to the MHC class I pathway provides an additional mechanism by which HIV-1 evades the CTL-mediated immune response.
Kutsch
O
O
Institute of Immunobiology and Virology, The Julius-Maximilians University, Würzburg, Germany. okutsch@uab.edu
Vey
T
T
Kerkau
T
T
Hünig
T
T
Schimpl
A
A
D018528:ATP-Binding Cassette Transporters / Q000502:physiology*; D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology; D017951:Antigen Presentation; D015536:Down-Regulation; D004721:Endoplasmic Reticulum / Q000378:metabolism; D015497:HIV-1 / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D021381:Protein Transport; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014762:Viral Interference
D018528:ATP-Binding Cassette Transporters; D015395:Histocompatibility Antigens Class I