title: Species-specific pheromonal compounds induce distinct conformational changes of pheromone binding protein subtypes from Antheraea polyphemus.
abstract: We have investigated the structural features of three pheromone binding protein (PBP) subtypes from Antheraea polyphemus and monitored possible changes induced upon interaction with the Antheraea pheromonal compounds 4E,9Z-14:Ac [(E4,Z9)-tetradecadienyl-1-acetate], 6E,11Z-16:Ac [(E6,Z11)-hexadecadienyl-1-acetate], and 6E,11Z-16:Al [(E6,Z11)-hexadecadienal]. Circular dichroism and second derivative UV-difference spectroscopy data demonstrate that the structure of subtype PBP1 significantly changes upon binding of 4E,9Z-14:Ac. The related 6E,11Z-16:Ac was less effective and 6E,11Z-16:Al showed only a small effect. In contrast, in subtype PBP2 pronounced structural changes were only induced by the 6E,11Z-16:Al, and the subtype PBP3 did not show any considerable changes in response to the pheromonal compounds. The UV-spectroscopic data suggest that histidine residues are likely to be involved in the ligand-induced structural changes of the proteins, and this notion was confirmed by site-directed mutagenesis experiments. These results demonstrate that appropriate ligands induce structural changes in PBPs and provide evidence for ligand specificity of these proteins.
authors.affiliation: University of Hohenheim, Institute of Physiology, Garbenstrasse 30, 70599 Stuttgart, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Mohl
authors.forename: Heinz
authors.initials: H
authors.lastname: Breer
authors.forename: Jürgen
authors.initials: J
authors.lastname: Krieger
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000187:drug effects*; D002942:Circular Dichroism; D007301:Insect Hormones / Q000737:chemistry; D019476:Insect Proteins / Q000737:chemistry* / Q000187:drug effects*; D036341:Intercellular Signaling Peptides and Proteins; D009036:Moths; D010675:Pheromones / Q000494:pharmacology*; D011487:Protein Conformation / Q000187:drug effects; D011994:Recombinant Proteins; D013045:Species Specificity
chemical_list: C094731:Aper-2 protein, Antheraea pernyi; D002352:Carrier Proteins; D007301:Insect Hormones; D019476:Insect Proteins; D036341:Intercellular Signaling Peptides and Proteins; D010675:Pheromones; D011994:Recombinant Proteins; C067715:APR-1 pheromone-binding protein, Antheraea pernyi