title: Interleukin-1beta and interleukin-1 receptor antagonist gene polymorphisms in ankylosing spondylitis.
abstract: OBJECTIVE
  Since ulcerative colitis and Crohn's disease, which are associated with ankylosing spondylitis (AS), have been found to be variably associated with the IL-1B and the IL-1RN genes encoding interleukin-1beta (IL-1beta) and the interleukin-1 receptor antagonist (IL-1ra), we have investigated whether these polymorphisms in IL-1B and IL-1RN are also involved in AS.
  
  
  METHODS
  DNA was isolated from peripheral blood of 106 patients with AS and 104 healthy controls. All patients and controls were Dutch Caucasians. Bi-allelic polymorphisms at positions +3,953 and -511 in the IL-1B gene, and a penta-allelic polymorphism in intron 2 of the IL-1RN gene were studied by polymerase chain reaction-based methods.
  
  
  RESULTS
  Allele IL-1RN*2 was significantly increased in AS (odds ratio=1.60; 95% confidence interval=1.20-2.80; P=0.031) compared with healthy controls, and independent from the polymorphism in loci IL-1B-511 and IL-1B+3,953. No significant associations were found between AS and the IL-1B-511 or IL-1B+3,953 polymorphisms.
  
  
  CONCLUSIONS
  Similar to other chronic inflammatory diseases, AS is associated with the IL-1RN*2 allele. Further studies are necessary to determine the biological significance of these findings in relation to susceptibility or severity of the disease.
authors.affiliation: The Jan van Breemen Institute, Amsterdam, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: van der Paardt
authors.forename: J B A
authors.initials: JB
authors.lastname: Crusius
authors.forename: M A
authors.initials: MA
authors.lastname: García-González
authors.forename: P
authors.initials: P
authors.lastname: Baudoin
authors.forename: P J
authors.initials: PJ
authors.lastname: Kostense
authors.forename: B Z
authors.initials: BZ
authors.lastname: Alizadeh
authors.forename: B A C
authors.initials: BA
authors.lastname: Dijkmans
authors.forename: A S
authors.initials: AS
authors.lastname: Peña
authors.forename: I E
authors.initials: IE
authors.lastname: van der Horst-Bruinsma
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D015796:HLA-B27 Antigen / Q000032:analysis; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors* / Q000235:genetics; D013167:Spondylitis, Ankylosing / Q000235:genetics* / Q000276:immunology
chemical_list: D015796:HLA-B27 Antigen; D007375:Interleukin-1; D017472:Receptors, Interleukin-1