title: Measurement of bisphenol A levels in human blood serum and ascitic fluid by HPLC using a fluorescent labeling reagent.
abstract: A sensitive column-switching HPLC method with fluorescence detection was developed for the determination of bisphenol A (BPA) in human blood serum and ascitic fluid samples. 4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) was used as the fluorescent label, and the excess reagent was removed by a column-switching technique. Liquid-liquid extraction with chloroform was used for the pretreatment of serum and ascitic fluid samples. BPA in both the samples could be determined in the concentration range of 0.1-7.0 ppb with the detection limit of 0.04 ppb at a signal-to-noise ratio of 3. The recoveries of BPA spiked to serum and ascitic fluid were 78.6 and 77.7%, respectively. The mean concentrations of BPA (n=9) in maternal and umbilical cord blood sera obtained from healthy pregnant women were 0.46+/-0.20 and 0.62+/-0.13 ppb, respectively. BPA levels (n=21) in blood sera and ascitic fluid obtained from the patients with sterility were also determined to be 0.46+/-0.20 and 0.56+/-0.19 ppb, respectively. Relationships of BPA concentrations were observed between maternal and umbilical cord blood serum samples (r=0.626), as well as blood serum and ascitic fluid samples (r=0.785).
authors.affiliation: Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan. n-kuro@net.nagasaki-u.sc.jp
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
authors.forename: Yumi
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: Yen
authors.initials: Y
authors.lastname: Sun
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Wada
authors.forename: Naoya
authors.initials: N
authors.lastname: Kishikawa
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Nakashima
authors.forename: Tsunehisa
authors.initials: T
authors.lastname: Makino
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakazawa
mesh_terms: D001202:Ascitic Fluid / Q000737:chemistry*; D001559:Benzhydryl Compounds; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D005456:Fluorescent Dyes / Q000032:analysis*; D006801:Humans; D010636:Phenols / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D011247:Pregnancy; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D005456:Fluorescent Dyes; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds