Pressor response to chemoreflex activation before and after microinjection of glycine into the NTS of awake rats.
Microinjection of glycine into the rostral (bilateral) and caudal (midline) commissural nucleus of the solitary tract (NTS) using three guide cannulas implanted in the direction of these sites produced an increase in mean arterial pressure (MAP) and abolished the pressor response to chemoreflex activation [potassium cyanide (n = 7)]. Strychnine, a glycine receptor antagonist, attenuated the increase in MAP, and in this new experimental condition (n = 5) the pressor response to chemoreflex activation was not altered. Considering that the effect of glycine on the attenuation of the pressor response to chemoreflex activation could be secondary to the increase in baseline MAP, in a third group of rats (n = 5) sodium nitroprusside infusion (intravenous) after microinjections of glycine into the NTS normalizes MAP. In this case, the pressor response to chemoreflex activation was similar to the control. These data show that glycine when microinjected bilaterally into the lateral commissural NTS as well as into the medial commissural NTS plays no major inhibitory role in the processing of the neurotransmission of the sympathoexcitatory component of the chemoreflex.
Pimentel
Franklin F
FF
Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, 14049-900, Ribeirão Preto, SP, Brazil.
Bonagamba
Leni G H
LG
Machado
Benedito H
BH
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D002628:Chemoreceptor Cells / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005998:Glycine / Q000494:pharmacology*; D018684:Glycine Agents / Q000494:pharmacology; D006339:Heart Rate / Q000187:drug effects*; D008297:Male; D009599:Nitroprusside / Q000494:pharmacology; D011190:Potassium Cyanide / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017552:Solitary Nucleus / Q000033:anatomy & histology / Q000187:drug effects*; D013331:Strychnine / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology; D014851:Wakefulness
D004791:Enzyme Inhibitors; D018684:Glycine Agents; D014665:Vasodilator Agents; D009599:Nitroprusside; D013331:Strychnine; D011190:Potassium Cyanide; D005998:Glycine