title: Detection of rice grassy stunt tenuivirus nonstructural proteins p2, p5 and p6 from infected rice plants and from viruliferous brown planthoppers.
abstract: The genome of Rice grassy stunt virus (RGSV) consists of 6 ambisense RNA segments, among which RNAs 1, 2, 5 and 6 are equivalent to RNAs 1, 2, 3 and 4, respectively, of Rice stripe virus, the type species of the genus Tenuivirus. The RGSV 36-kDa nucleocapsid protein (N) is encoded on the complementary strand of RNA 5. Here, we studied accumulation of three nonstructural proteins, a 23-kDa p2 protein encoded on vRNA 2 (virus genomic strand), a 22-kDa p5 protein encoded on vRNA 5, and a 21-kDa p6 protein encoded on vRNA 6, from RGSV-infected rice leaf tissues and from viruliferous vector insects (brown planthopper, Nilaparvata lugens) by Western blot analyses. p2, p5 and p6 were detected from RGSV-infected rice leaf homogenates; p2 was detected mostly in the cytoplasmic soluble fraction but also a small amount was detected in the cell-wall, organelle-enriched and crude membrane fractions; p5 and p6 were detected from the cytoplasmic soluble fraction in large amounts. Among individual nymphs of N. lugens raised on RGSV-infected rice leaves, only 20% of insects were positive with the N protein. A large amount of p5 was detected from all the N-positive insects. Small amounts of p2 and p6 were detected only from a subset of the N- and p5-positive insects. p5 may have an essential role in virus infection in both plant and insect hosts, whereas p2 may function in plants such as a cell-to-cell movement protein.
authors.affiliation: Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, Japan.
authors.forename: P
authors.initials: P
authors.lastname: Chomchan
authors.forename: G J
authors.initials: GJ
authors.lastname: Miranda
authors.forename: Y
authors.initials: Y
authors.lastname: Shirako
mesh_terms: D000818:Animals; D015153:Blotting, Western; D007303:Insect Vectors; D007313:Insecta / Q000821:virology*; D008970:Molecular Weight; D019590:Nucleocapsid Proteins / Q000032:analysis; D009758:Nymph / Q000821:virology; D012275:Oryza / Q000821:virology*; D010935:Plant Diseases / Q000821:virology; D010936:Plant Extracts; D018515:Plant Leaves / Q000821:virology; D013347:Subcellular Fractions / Q000821:virology; D029364:Tenuivirus / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000032:analysis* / Q000737:chemistry
chemical_list: D019590:Nucleocapsid Proteins; D010936:Plant Extracts; D017361:Viral Nonstructural Proteins