Harvesting of CD34 antigen-expressing cells with a new programme for the collection of mononuclear cells with use of the Amicus (Baxter) blood cell separator.
A study was performed to evaluate a new programme for peripheral blood stem cell (PBSC) collection with the use of the Amicus (Baxter) blood cell separator. Healthy donors (n = 9) and oncology patients (n = 21) scheduled for PBSC transplant were studied. Ten PBSC harvests were performed in the donors and 30 in the patients. A median of 6.37 x 106 CD34+ cells per kg recipient body weight (range 3.08-11.06 x 106) were collected from the donors in a product weight of 169.5 g (118-186). From the patients, 6.26 x 106 CD34+ cells per kg body weight (range 0.2-53.6 x 106) were harvested in a product weighing 121.5 g (range 92-190). The median platelet contamination was 0.93 x 1011 (range 0.45-1.23 x 1011) per donor product and 0.2 x 1011 (range 0.05-0.86 x 1011) per patient product. No severe side effects were observed during or after the PBSC collection procedures.
Moog
R
R
Institut für Transfusionsmedizin, Universitätsklinikum Essen, Essen, Germany. rainer.moog@uni-essen.de
D000328:Adult; D000368:Aged; D018952:Antigens, CD34; D002404:Catheterization / Q000009:adverse effects; D005260:Female; D006412:Hematopoietic Stem Cells; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation*; D007963:Leukocytes, Mononuclear; D008297:Male; D008875:Middle Aged; D012984:Software
D018952:Antigens, CD34