title: Relationship between DNA mismatch repair genes expression, Ku-genes expression and ploidy-related parameters in the progression of pigmented lesions of the skin.
abstract: Defects of DNA repair systems in cutaneous tumours are related to DNA mismatch repair genes (MLH1, MSH2, PMS1, PMS2) and Ku70/80 genes involved in double- strand repair. In this study we investigated the statistical relationship between these systems and DNA-ploidy-related parameters in 19 naevus cell naevi, 23 lentigos maligna, 76 primary melanomas and 31 melanoma metastases, applying the correlation coefficient according to Spearman. In naevi significant correlations were found between Ku70/80 gene expression and some ploidy-related parameters. In lentigos, additionally, some significant correlations between the expression of DNA mismatch repair genes were found. Similar results were demonstrated for primary melanomas. In metastases no one significant correlation between DNA mismatch repair genes and Ku-genes was present. We postulate that DNA mismatch repair genes and Ku70/80 genes are functionally independent and that some of them are able to influence ploidy-related parameters.
authors.affiliation: Department of Cytopathology, Department of Gastroenteropathology, Georg August University Göttingen, 37075 Göttingen, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Korabiowska
authors.forename: Michael
authors.initials: M
authors.lastname: Tscherny
authors.forename: Jerzy
authors.initials: J
authors.lastname: Stachura
authors.forename: Ilka
authors.initials: I
authors.lastname: Ruschenburg
authors.forename: Carlos
authors.initials: C
authors.lastname: Cordon-Cardo
authors.forename: Ulrich
authors.initials: U
authors.lastname: Brinck
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D034961:Antigens, Nuclear / Q000235:genetics* / Q000378:metabolism; D020137:Base Pair Mismatch; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002452:Cell Count; D004265:DNA Helicases; D004260:DNA Repair; D045643:DNA Repair Enzymes; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006801:Humans; D018327:Hutchinson's Melanotic Freckle / Q000235:genetics / Q000473:pathology; D019044:Image Cytometry; D007150:Immunohistochemistry; D000072200:Ku Autoantigen; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D008297:Male; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism / Q000556:secondary; D008875:Middle Aged; D000070976:Mismatch Repair Endonuclease PMS2; D000070957:MutL Protein Homolog 1; D000070956:MutL Proteins; D051718:MutS Homolog 2 Protein; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D009506:Nevus / Q000235:genetics / Q000473:pathology; D009687:Nuclear Proteins; D011003:Ploidies; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D034961:Antigens, Nuclear; D002352:Carrier Proteins; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; C501486:MLH1 protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; C088996:PMS1 protein, human; D011518:Proto-Oncogene Proteins; D000251:Adenosine Triphosphatases; C094934:PMS2 protein, human; C497172:MSH2 protein, human; D000070976:Mismatch Repair Endonuclease PMS2; D000070957:MutL Protein Homolog 1; D000070956:MutL Proteins; D051718:MutS Homolog 2 Protein; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen; D045643:DNA Repair Enzymes