Release of transforming growth factor beta-1 in a vestibular schwannoma cell line.
Vestibular schwannoma (VS) often displays an irregular growth pattern due to factors which remain unknown. In this study, VS cell lines from tissue removed during surgery were cultured in order to assess the presence of transforming growth factor beta-1 (TGFbeta-1) a multifunctional polypeptide that has different pleiotropic effects on various tissues. The antibody-binding inhibition assay technique was used. The proliferation rate of VS cell lines in vitro was assessed by [3H]thymidine incorporation, with determinations carried out on days 0, 1, 2 and 4 after removal of fetal calf serum (FCS) from the medium. After 48 h of FCS deprivation, cell growth decreased. TGFbeta-1 release also decreased progressively 24 h after removing FCS from the medium, reaching an overall decrease of 50% after 3 days. The cell culturing procedure appeared suitable for VS cells, in that VS cultures were viable for at least 4 months. TGFbeta-1 was initially shown to be released in significant amounts, but also to decrease progressively to values five times lower after 4 days. These data thus support the possibility of a significant association between TGFbeta-1 release and VS cell replication.
Bizzarri
Mariano
M
Institute of Experimental Medicine, University of Rome La Sapienza, Rome, Italy.
Filipo
Roberto
R
Valente
Maria Grazia
MG
Bernardeschi
Daniele
D
Ronchetti
Francesco
F
Monini
Simonetta
S
Chiappini
Ilaria
I
Barbara
Maurizio
M
D000936:Antigen-Antibody Complex / Q000097:blood; D002465:Cell Movement / Q000502:physiology; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D046508:Culture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009464:Neuroma, Acoustic / Q000378:metabolism* / Q000473:pathology*; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000097:blood* / Q000378:metabolism; D053773:Transforming Growth Factor beta1
D000936:Antigen-Antibody Complex; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1