Nitric oxide inhibits cysteine proteinases and alcohol dehydrogenase 2 of Entamoeba histolytica.
Nitric oxide is involved in the neutrophil and macrophage killing of the protozoan parasite Entamoeba histolytica. In the present study, we found that cysteine proteinases, significant contributors to amebic virulence and alcohol dehydrogenase 2, an enzyme absolutely required for the survival of the parasite, are both significantly inhibited by S-nitroso-glutathione, a physiological nitric oxide donor, within the concentration range 0.5-2.0 m M.
Siman-Tov
Rama
R
Department of Molecular Microbiology, The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, P.O.Box 9649, 31096 Haifa, Israel.
Ankri
Serge
S
D000426:Alcohol Dehydrogenase / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D003546:Cysteine Endopeptidases / Q000032:analysis / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000187:drug effects* / Q000201:enzymology*; D004749:Entamoebiasis / Q000188:drug therapy; D006790:Host-Parasite Interactions; D009569:Nitric Oxide / Q000494:pharmacology; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D026422:S-Nitrosoglutathione / Q000494:pharmacology*
D020030:Nitric Oxide Donors; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; D000426:Alcohol Dehydrogenase; D003546:Cysteine Endopeptidases