Characterization of a multipotent neural progenitor cell line cloned from an adult p53-/- mouse cerebellum.
Here we report developmental characteristics of clonal cell line 2Y6f1, which was established from an adult p53(-/-) mouse cerebellum. 2Y6f1 began as a homogeneous population of small polygonal epithelial cells, but during passages it gradually became heterogeneous, containing cells of varying size and shape that expressed either neuron- or astrocyte-specific proteins. Supplements to the culture medium altered the levels of some of the cell type markers. For example, addition of insulin increased expression of neurofilaments, while cholera toxin increased that of glial fibrillary acidic protein. In a colony assay, 2Y6f1 cells gave rise to both homogeneous and heterogeneous colonies, consistent with the idea that they contained multipotent neural progenitor cells. Establishment of subclones that were exclusively neuronal or astroglial in differentiation further supported the conclusion that 2Y6f1 cells have many features that may qualify them as bona fide stem cells and make them a useful new model in neural stem cell biology.
Ohkawara
Bisei
B
Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, City, Noda, Chiba 278-8510, Japan.
Okuno
Misako
M
Ishii
Takashi
T
Horiuchi
Makoto
M
Tomooka
Yasuhiro
Y
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D019070:Cell Lineage; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology* / Q000378:metabolism*; D002999:Clone Cells; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D006133:Growth Substances / Q000494:pharmacology; D007150:Immunohistochemistry; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology / Q000637:transplantation; D009154:Mutation; D011972:Receptor, Insulin / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency
D015415:Biomarkers; D006133:Growth Substances; D007328:Insulin; D016159:Tumor Suppressor Protein p53; D066246:ErbB Receptors; D011972:Receptor, Insulin