Molecular and pharmacological diversity of the kinin B1 receptor.
The pharmacological properties of the kinin B1 receptor in binding the endogenous kinin peptides are known to differ across species. Molecular cloning has revealed that these pharmacological differences arise from the diversity within the BDKRB gene. In this report, the molecular diversity of the human BDKRB1 gene is expanded by the identification of eight single nucleotide polymorphisms (SNPs) in the coding sequence of the receptor, three of which change the amino acid sequence of the receptor. The molecular cloning and pharmacological characterization of two primate B1 receptors, rhesus and African Green monkey, reveals that they exhibit the same high degree of selectivity for des-Arg10 kallidin (Lys-bradykinin) relative to des-Arg9 bradykinin that is observed with the human kinin B1 receptor. Previous mutagenesis studies of the human B1 receptor have implicated extracellular domain (EC) IV in conferring this selectivity for des-Arg10 kallidin, by interacting with the N-terminal Lys residue of the peptide. The pharmacological analysis of chimeric B1 receptors, in which EC-IV of the human B1 receptor is replaced with the corresponding domain of either rat or dog, supports the proposal that EC-IV is an important determinant in conferring ligand selectivity.
Hess
J Fred
JF
Department of Neuroscience, Merck Research Laboratory, WP26A-3000, West Point, PA 19486, USA. fred_hess@merck.com
Hey
Patricia J
PJ
Chen
Tsing-Bau
TB
Pettibone
Douglas J
DJ
Chang
Ray S L
RS
D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004285:Dogs; D005787:Gene Frequency; D006801:Humans; D007609:Kallidin / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D051381:Rats; D043783:Receptor, Bradykinin B1; D018002:Receptors, Bradykinin / Q000235:genetics* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013045:Species Specificity
D043783:Receptor, Bradykinin B1; D018002:Receptors, Bradykinin; D011993:Recombinant Fusion Proteins; C024626:bradykinin, des-Arg(9)-; D007609:Kallidin; C052787:kallidin, des-Arg(10)-; D001920:Bradykinin