title: Synthesis and characterization of nitric oxide generative polyrotaxane.
abstract: L-Arginine was immobilized into a supramolecular-structured polyrotaxane to examine the generation of nitric oxide, with a view to improving antithrombosis and the blood compatibility of polymeric biomaterials. L-Arginine was immobilized to the hydroxyl groups of alpha-cyclodextrins in the polyrotaxane via an ester linkage, and the nitric oxide generation and L-arginine release behavior were characterized. L-Arginine-immobilized polyrotaxane was insoluble in water, but was found to generate nitric oxide when placed in Tris-HCI buffer supplemented with activators. L-Arginine-immobilized polyrotaxane exhibited sustained nitric oxide generation for a period of 250 h. L-Arginine was completely released by non-enzymatic hydrolysis from 200 h to 700 h, with a lag-time for the first 200 h. Consequently, after the generation of nitric oxide and the release of L-arginine from the L-arginine-immobilized polyrotaxane, the residual component will be a polyrotaxane with superior biocompatibility and mechanical properties. These results suggest that L-arginine-immobilized polyrotaxane can be useful in a wide range of medical applications, including use as a nitric oxide generative system for antithrombosis, coating and blending materials of hydrophobic extracorporeal circuits, and implantable catheters.
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology, Tatsunokuchi, Ishikawa 923-1292, Japan.
authors.forename: Won Kyu
authors.initials: WK
authors.lastname: Lee
authors.forename: Junichi
authors.initials: J
authors.lastname: Kobayashi
authors.forename: Tooru
authors.initials: T
authors.lastname: Ooya
authors.forename: Ki Dong
authors.initials: KD
authors.lastname: Park
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Yui
mesh_terms: D001120:Arginine / Q000378:metabolism; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000378:metabolism; D002956:Citrulline / Q000378:metabolism; D003505:Cyclodextrins / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D020442:Poloxamer / Q000138:chemical synthesis* / Q000378:metabolism*; D043862:Rotaxanes; D047391:alpha-Cyclodextrins
chemical_list: D001672:Biocompatible Materials; D003505:Cyclodextrins; D043862:Rotaxanes; D047391:alpha-Cyclodextrins; C475673:polyrotaxane; D020442:Poloxamer; D002956:Citrulline; D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase; C032613:alpha-cyclodextrin