title: Study of the nervous tissue development in rainbow trout (Oncorhynchus mykiss) embryos treated with oxytetracycline.
abstract: The purpose of this study was to discover whether the use of different doses of oxytetracycline causes any alteration in the development of nervous tissue in rainbow trout embryos. Five thousands eggs of females rainbow trout were divided into five groups. One group acted as control and the other four were administered with one of four doses of oxytetracycline, 0.025, 0.050, 0.100, or 0.201 microM, at the moment of fertilization. The eggs were incubated under pisciculture conditions to just before being ready to spring off. From the 10th day, 10-egg samples were taken regularly and fixed. Five were processed for histological techniques and stained with haematoxylin and eosin, cresyl fast violet and silver, the other five were homogenized for antibiotic detection. Histological alterations appeared in 37-day-old embryos, with an abnormal migration of the neuroblasts to the marginal layer of the neural cord, and alterations in the development of the lens and eye layers. Some embryos showed abnormal curvature of the spinal cord but these changes were not statistically significant.
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, University of Concepción, Chillán, Chile. paarias@udec.cl
authors.forename: P
authors.initials: P
authors.lastname: Arias
authors.forename: L F
authors.initials: LF
authors.lastname: Pinochet
authors.forename: A
authors.initials: A
authors.lastname: Disi
mesh_terms: D000367:Age Factors; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000294:innervation*; D047108:Embryonic Development; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D017686:Oncorhynchus mykiss / Q000196:embryology*; D010118:Oxytetracycline / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010118:Oxytetracycline