title: Extraction of phyllanthusols A and B from Phyllanthus acidus and analysis by capillary electrophoresis.
abstract: Extracts of roots Phyllanthus acidus were examined by free zone capillary electrophoresis, micellar electrokinetic chromatography (MEKC), and MEKC using the sweeping technique which involves application of a negative potential to the inlet end of the capillary and very much longer than conventional injection times. The latter technique, using a buffer of 50 mM sodium dihydrogen phosphate (pH 2) containing 80 mM sodium dodecylsulphate and 30% methanol was found to allow complete resolution of the active constituents of P. acidus, phyllanthusols A and B, from each other and from other extracted components in under 30 min. Several other components could be detected when hydrodynamic injection times of 500 s were used. The separation, combined with an appropriate extraction procedure and using an internal standard of proguanil, permitted quantification of both phyllanthusols. Calibrations were linear over the range 2-8 micrograms/mL for phyllanthusol A, and 1-4 micrograms/mL for phyllanthusol B. Within-day and day-to-day repeatability RSDs were below 10%, and the precision of extraction RSD was around 14%. The limits of quantification and detection were 0.55 and 0.24 microgram/mL, respectively.
authors.affiliation: School of Pharmacy, The Robert Gordon University, Schoolhill, Aberdeen AB10 1FR, Scotland, UK. d.g.durham@rgu.ac.uk
authors.forename: D G
authors.initials: DG
authors.lastname: Durham
authors.forename: R G
authors.initials: RG
authors.lastname: Reid
authors.forename: J
authors.initials: J
authors.lastname: Wangboonskul
authors.forename: S
authors.initials: S
authors.lastname: Daodee
mesh_terms: D001572:Benzofurans / Q000737:chemistry* / Q000302:isolation & purification; D004187:Disaccharides / Q000737:chemistry* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods; D015394:Molecular Structure; D029803:Phyllanthus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry* / Q000302:isolation & purification; D013141:Spiro Compounds / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D001572:Benzofurans; D004187:Disaccharides; D010936:Plant Extracts; D012717:Sesquiterpenes; D013141:Spiro Compounds; C414101:phyllanthusol A; C414102:phyllanthusol B