title: B-cell infiltration and frequency of cytokine producing cells differ between localized and disseminated human cutaneous leishmaniases.
abstract: Biopsies from human localized cutaneous lesions (LCL n = 7) or disseminated lesions (DL n = 8) cases were characterized according to cellular infiltration,frequency of cytokine (IFN-gamma, TNF-alpha) or iNOS enzyme producing cells. LCL, the most usual form of the disease with usually one or two lesions, exhibits extensive tissue damage. DL is a rare form with widespread lesions throughout the body; exhibiting poor parasite containment but less tissue damage. We demonstrated that LCL lesions exhibit higher frequency of B lymphocytes and a higher intensity of IFN-gamma expression. In both forms of the disease CD8+ were found in higher frequency than CD4+ T cells. Frequency of TNF-alpha and iNOS producing cells, as well as the frequency of CD68+ macrophages, did not differ between LCL and DL. Our findings reinforce the link between an efficient control of parasite and tissue damage, implicating higher frequency of IFN-gamma producing cells, as well as its possible counteraction by infiltrated B cells and hence possible humoral immune response in situ.
authors.affiliation: Faculdade de Medicina, Universidade Federal da Bahias, Salvador, BA, Brasil.
authors.forename: M G S
authors.initials: MG
authors.lastname: Vieira
authors.forename: F
authors.initials: F
authors.lastname: Oliveira
authors.forename: S
authors.initials: S
authors.lastname: Arruda
authors.forename: A L
authors.initials: AL
authors.lastname: Bittencourt
authors.forename: A A
authors.initials: AA
authors.lastname: Barbosa
authors.forename: M
authors.initials: M
authors.lastname: Barral-Netto
authors.forename: A
authors.initials: A
authors.lastname: Barral
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000276:immunology*; D016516:CD4-CD8 Ratio; D002648:Child; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D016898:Interferon-alpha / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D018709:Statistics, Nonparametric
chemical_list: D016207:Cytokines; D016898:Interferon-alpha; D007371:Interferon-gamma; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II