On-line determination of biomass in a microalga bioreactor using a novel computerized flow injection analysis system.
A flow injection analysis (FIA) device has been developed, which is able to assay successfully for biomass in a microalga bioreactor. The device is fully computerized and is operated via diluting small aliquots of the culture followed by measuring optical density (OD); this figure is then accurately correlated with biomass, in terms of both cell number and ash-free dry weight, during the entire culture time. Furthermore, the device is not expensive, is highly versatile, and is easy to operate owing to specifically developed, user-friendly software. The growth rate and biomass productivity of Pavlova lutheri, cultivated under batch and semicontinuous modes, were monitored as experimental testing model.
Meireles
Luís A
LA
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr António Bernardino de Almeida, P-4200-072 Porto, Portugal.
Azevedo
José L
JL
Cunha
João P
JP
Malcata
F Xavier
FX
D018533:Biomass; D019149:Bioreactors; D003201:Computers; D056890:Eukaryota / Q000166:cytology / Q000254:growth & development*; D007700:Kinetics; D009862:Online Systems; D013057:Spectrum Analysis