title: CDKN2A region polymorphism and genetic susceptibility to melanoma in the melim swine model of familial melanoma.
abstract: Some herds of miniature swine are genetically predisposed to cutaneous melanoma. To test if swine melanoma susceptibility could be linked to the CDKN2A gene, which is involved in a proportion of 9p21-linked human familial melanoma, we performed a genetic analysis of miniature pigs of the MeLiM strain. F(1) and backcross animals were generated by crossing 1 MeLiM boar with healthy Duroc sows. We isolated the swine CDKN2A gene and characterized a linked informative microsatellite marker, the S0644 marker. Using this marker and 2 flanking markers, we analyzed the segregation of the CDKN2A gene in a 3-generation pedigree. Allelic association, linkage analysis and haplotype analysis of these data led to exclusion of the CDKN2A gene as a candidate for melanoma susceptibility. Nonetheless, this analysis suggests an association with the swine 1q25 chromosomal region, which is homologous to the human 9p21 region.
authors.affiliation: Laboratoire de Radiobiologie et d'Etude du Génome, Commíssariat à l'Energie Atomique-Institut National de Recherche Agronomique, Jouy-en-Josas, France. lechalon@diamant.jouy.inra.fr
authors.forename: Catherine
authors.initials: C
authors.lastname: Le Chalony
authors.forename: Christine
authors.initials: C
authors.lastname: Renard
authors.forename: Silvia
authors.initials: S
authors.lastname: Vincent-Naulleau
authors.forename: Françoise
authors.initials: F
authors.lastname: Crechet
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Leplat
authors.forename: Yves
authors.initials: Y
authors.lastname: Tricaud
authors.forename: Vratislav
authors.initials: V
authors.lastname: Horak
authors.forename: Joseph
authors.initials: J
authors.lastname: Gruand
authors.forename: Pascale
authors.initials: P
authors.lastname: Le Roy
authors.forename: Gérard
authors.initials: G
authors.lastname: Frelat
authors.forename: Claudine
authors.initials: C
authors.lastname: Geffrotin
mesh_terms: D000483:Alleles; D000818:Animals; D020090:Chromosome Segregation; D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D019942:Genes, p16; D008040:Genetic Linkage; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D008545:Melanoma / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D018895:Microsatellite Repeats; D010375:Pedigree; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012878:Skin Neoplasms / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D013553:Swine Diseases / Q000235:genetics*; D013556:Swine, Miniature
chemical_list: D017931:DNA Primers; D004273:DNA, Neoplasm; D005819:Genetic Markers