Low resolution structure determination shows procollagen C-proteinase enhancer to be an elongated multidomain glycoprotein.
Procollagen C-proteinase enhancer (PCPE) is an extracellular matrix glycoprotein that can stimulate the action of tolloid metalloproteinases, such as bone morphogenetic protein-1, on a procollagen substrate, by up to 20-fold. The PCPE molecule consists of two CUB domains followed by a C-terminal NTR (netrin-like) domain. In order to obtain structural insights into the function of PCPE, the recombinant protein was characterized by a range of biophysical techniques, including analytical ultracentrifugation, transmission electron microscopy, and small angle x-ray scattering. All three approaches showed PCPE to be a rod-like molecule, with a length of approximately 150 A. Homology modeling of both CUB domains and the NTR domain was consistent with the low-resolution structure of PCPE deduced from the small angle x-ray scattering data. Comparison with the low-resolution structure of the procollagen C-terminal region supports a recently proposed model (Ricard-Blum, S., Bernocco, S., Font, B., Moali, C., Eichenberger, D., Farjanel, J., Burchardt, E. R., van der Rest, M., Kessler, E., and Hulmes, D. J. S. (2002) J. Biol. Chem. 277, 33864-33869) for the mechanism of action of PCPE.
Bernocco
Simonetta
S
Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS-UCBL1, 69367 Lyon cedex 07, France.
Steiglitz
Barry M
BM
Svergun
Dmitri I
DI
Petoukhov
Maxim V
MV
Ruggiero
Florence
F
Ricard-Blum
Sylvie
S
Ebel
Christine
C
Geourjon
Christophe
C
Deleage
Gilbert
G
Font
Bernard
B
Eichenberger
Denise
D
Greenspan
Daniel S
DS
Hulmes
David J S
DJ
D000595:Amino Acid Sequence; D002460:Cell Line; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D013058:Mass Spectrometry; D008854:Microscopy, Electron; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000378:metabolism; D012542:Scattering, Radiation; D017386:Sequence Homology, Amino Acid; D014461:Ultracentrifugation; D014965:X-Rays
D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; C491555:PCOLCE protein, human; D011994:Recombinant Proteins