title: Effects of homocysteine on the cellular responses of murine endothelial cells cultured in vitro.
abstract: Homocysteine, derived from the metabolism of methionine, is claimed as a proatherogenic factor that leads to vascular dysfunction. To gain better insight into the molecular mechanisms involved, homocysteine was tested on a model of murine endothelial cells cultured in vitro, using a prototype DNA chip. The DNA chip was designed to follow the expression at the mRNA level of some major proinflammatory genes; TNF-alpha was used as a positive control.
authors.affiliation: Department of Biology, Laboratory of Cellular Biochemistry and Biology, University of Namur (FUNDP), Namur, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Tacheny
authors.forename: S
authors.initials: S
authors.lastname: dos Santos
authors.forename: A-C
authors.initials: AC
authors.lastname: Huet
authors.forename: M
authors.initials: M
authors.lastname: van Steenbrugge
authors.forename: C
authors.initials: C
authors.lastname: Michiels
authors.forename: P
authors.initials: P
authors.lastname: Holvoet
authors.forename: M
authors.initials: M
authors.lastname: Raes
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D006710:Homocysteine / Q000633:toxicity*; D007249:Inflammation / Q000235:genetics; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D014409:Tumor Necrosis Factor-alpha; D006710:Homocysteine