Automated analysis of polyethylene glycol-induced inhibition of P-glycoprotein activity in vitro.
Previous studies in our laboratories have shown that commonly used polyethoxylated pharmaceutical excipients inhibit P-glycoprotein activity in cell culture models of the intestinal mucosa. The results presented in this technical note show that the TECAN Genesis robotic workstation can be utilized to automate cellular transport studies for evaluating excipient effects on P-glycoprotein activity in vitro and for estimating the permeation of drug-like molecules across cell monolayers.
Hugger
Erin D
ED
Pharmaceutical Chemistry Department, The University of Kansas, Lawrence, Kansas 66047, USA. Erin_D_Hugger@gsk.com
Cole
Clayton J
CJ
Raub
Thomas J
TJ
Burton
Philip S
PS
Borchardt
Ronald T
RT
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D018938:Caco-2 Cells; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D006801:Humans; D011092:Polyethylene Glycols / Q000493:pharmacokinetics*; D012371:Robotics / Q000295:instrumentation / Q000379:methods*; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000379:methods*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D011092:Polyethylene Glycols