A novel solid support for synthesis of oligonucleotide 3'-phosphorothioate monoesters.
A new reagent immobilized on solid support allowing for solid-phase synthesis of oligonucleotides with a 3'-terminal phosphorothioate monoester is described. The support is compatible with phosphoramidite chemistry for automated oligonucleotide synthesis. Final deprotection with ammonia under standard conditions leads to oligonucleotide 3'-terminal phosphorothioate.
Cheruvallath
Zacharia S
ZS
Isis Pharmaceuticals, 2292 Faraday Avenue, Carlsbad, CA 92008, USA.
Cole
Douglas L
DL
Ravikumar
Vasulinga T
VT
D000641:Ammonia / Q000737:chemistry; D019075:Electrophoresis, Capillary; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009946:Organothiophosphorus Compounds / Q000138:chemical synthesis*
D007202:Indicators and Reagents; D009841:Oligonucleotides; D009946:Organothiophosphorus Compounds; D000641:Ammonia