The biomimetics of bone: engineered glass-ceramics a paradigm for in vitro biomineralization studies.
In this study, we investigated the behavior of fetal rat osteoblasts cultured up to 23 days on a bioactive apatite-wollastonite glass-ceramic (AW) and on the same material on which a carbonated apatite layer was formed by a biomimetic process (AWa). The specific activity of alkaline phosphatase activity was about 30% increased on AWa compared to AW disks at the last day of culture. Scanning electron microscopic (SEM) observations of the material surfaces after scrapping off the cell layers revealed that mineralized bone nodules remained attached to both surfaces but in larger numbers on AWa. The AWa/bone interfaces were also analyzed after fracturing the disks and by transmission electron microscopy (TEM). All these results indicated the importance of the surface composition in supporting differentiation of osteogenic cells and the subsequent apposition of bone matrix. Furthermore, prefabrication of a biological apatite layer by a biomimetic method could improve our knowledge of biomineralization processes and could find application as bone-repairing material.
Loty
Christine
C
Laboratoire Biologie-Orofaciale et Pathologie, INSERM EMI-U0110, Université Paris 7, IFR-58, Institut Biomédical des Cordeliers, Esc. E-2è ét., 15-21 rue de l'Ecole de Médecine, 75270 Paris, France.
Sautier
Jean-Michel
JM
Loty
Sabine
S
Hattar
Susan
S
Asselin
Audrey
A
Oboeuf
Martine
M
Kokubo
Tadashi
T
Kim
Huyn-Min
HM
Boulekbache
Habib
H
Berdal
Ariane
A
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001031:Apatites / Q000494:pharmacology; D001698:Biomedical Engineering; D040761:Biomimetic Materials / Q000378:metabolism / Q000494:pharmacology; D001842:Bone and Bones; D002113:Calcification, Physiologic; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002516:Ceramics / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats / Q000196:embryology; D012824:Silicic Acid / Q000494:pharmacology
D001031:Apatites; C077777:apatite-wollastonite-containing glass ceramic; D012824:Silicic Acid; D000469:Alkaline Phosphatase