title: Correlation between the percentages of myeloblasts in bone marrow obtained by flow cytometry and manual counting on glass slide smears in 74 hematologic patients.
abstract: To investigate reliability of calculating percentage of myeloblasts by flow cytometric method, data were obtained from 74 hematologic patients (76 paired data). Myeloblast counts obtained by manual count versus flow cytometry were compared. Our data show that the percentage of myeloblasts in the bone marrow obtained with flow cytometric method correlates well with manual count (correlation coefficient is 0.9912). A very high correlation coefficient means that reliable percentage of myeloblasts in the bone marrow can be obtained by either method alone. Flow cytometry is a useful adjunct (or quality control) to validate manual myeloblast count and vice versa.
authors.affiliation: tomislav.jelic@camcare.com
authors.forename: Tomislav M
authors.initials: TM
authors.lastname: Jelic
authors.forename: Paul F
authors.initials: PF
authors.lastname: Mellen
authors.forename: Kevin
authors.initials: K
authors.lastname: Zavieh
authors.forename: Josephine C
authors.initials: JC
authors.lastname: Roque
authors.forename: Shawn P
authors.initials: SP
authors.lastname: Farren
authors.forename: Bo
authors.initials: B
authors.lastname: Jin
authors.forename: Naushaba
authors.initials: N
authors.lastname: Ahmad
authors.forename: Oscar C
authors.initials: OC
authors.lastname: Estallila
authors.forename: Ho Haung
authors.initials: HH
authors.lastname: Chang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001399:Azure Stains; D001854:Bone Marrow Cells / Q000473:pathology*; D001856:Bone Marrow Examination / Q000379:methods* / Q000592:standards; D002452:Cell Count; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006402:Hematologic Diseases / Q000473:pathology*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D022423:Myeloid Cells / Q000473:pathology*; D015203:Reproducibility of Results
chemical_list: D001399:Azure Stains