The distribution and morphological characteristics of catecholaminergic cells in the brain of monotremes as revealed by tyrosine hydroxylase immunohistochemistry.
The present study describes the distribution and cellular morphology of catecholaminergic neurons in the CNS of two species of monotreme, the platypus (Ornithorhynchus anatinus) and the short-beaked echidna (Tachyglossus aculeatus). Tyrosine hydroxylase immunohistochemistry was used to visualize these neurons. The standard A1-A17, C1-C3 nomenclature was used for expediency, but the neuroanatomical names of the various nuclei have also been given. Monotremes exhibit catecholaminergic neurons in the diencephalon (A11, A12, A13, A14, A15), midbrain (A8, A9, A10), rostral rhombencephalon (A5, A6, A7), and medulla (A1, A2, C1, C2). The subdivisions of these neurons are in general agreement with those of other mammals, and indeed other amniotes. Apart from minor differences, those being a lack of A4, A3, and C3 groups, the catecholaminergic system of monotremes is very similar to that of other mammals. Catecholaminergic neurons outside these nuclei, such as those reported for other mammals, were not numerous with occasional cells observed in the striatum. It seems unlikely that differences in the sleep phenomenology of monotremes, as compared to other mammals, can be explained by these differences. The similarity of this system across mammalian and amniote species underlines the evolutionary conservatism of the catecholaminergic system.
Manger
P R
PR
Department of Psychiatry, University of California, Los Angeles, Neurobiology Research 151A3, Sepulveda VAMC, North Hills, Calif., USA. mangerpr@anatomy.wits.ac.za
Fahringer
H M
HM
Pettigrew
J D
JD
Siegel
J M
JM
D000818:Animals; D005075:Biological Evolution; D001921:Brain / Q000033:anatomy & histology*; D001931:Brain Mapping; D002799:Cholinergic Fibers / Q000000981:diagnostic imaging*; D004298:Dopamine / Q000502:physiology; D004837:Epinephrine / Q000502:physiology; D009415:Nerve Net / Q000033:anatomy & histology; D009474:Neurons / Q000000981:diagnostic imaging; D010802:Phylogeny; D010987:Platypus / Q000033:anatomy & histology*; D012890:Sleep / Q000502:physiology; D013045:Species Specificity; D004442:Tachyglossidae / Q000033:anatomy & histology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*; D014463:Ultrasonography
D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine; D004837:Epinephrine