Gene introgression into Coffea arabica by way of triploid hybrids (C. arabica x C. canephora).
Interspecific triploid hybrid plants between the tetraploid species Coffea arabica L. and the diploid species C. canephora P. were backcrossed to C. arabica. Although characterised by a low production and an important fruit dropping, all attempted crosses (ie, 6) generated BC(1) progenies. Flow cytometric analysis of the nuclear DNA content revealed that most of the BC1 individuals were nearly tetraploid. Among the male gametes produced by the interspecific triploid hybrids, those presenting a high number of chromosomes appeared strongly favoured. Only pollen mother cells having nearly 22 chromosomes were effective, the others leading to deficient endosperm and fruit dropping. Molecular markers (ie, microsatellite and AFLP) combined with evaluations of morphological characteristics and resistance to leaf rust were applied to verify the occurrence of gene transfer from C. canephora into C. arabica, and to estimate the amount of introgression present in BC(1) individuals. The results reveal a strong deficiency in the C. canephroa alleles indicating a severe counter-selection against the introgression of genetic material from C. canephora into C. arabica by way of triploid hybrids. However, introgressants displaying desirable traits such as a high resistance to leaf rust were obtained. The low level of introgression could be an advantage by facilitating the recovery of the recurrent parent and possibly reducing the number of required backcrosses. On the other hand, this could be a limitation when attempting the transfer of a complex trait or several simply inherited traits.
Herrera
J C
JC
Centro Nacional de Investigaciones de Cafe, CENICAFE, AP 2427, Manizales, Colombia.
Combes
M C
MC
Cortina
H
H
Alvarado
G
G
Lashermes
P
P
D040503:Coffea / Q000235:genetics*; D004247:DNA / Q000378:metabolism; D005819:Genetic Markers; D014644:Genetic Variation; D005854:Germ Cells; D006824:Hybridization, Genetic; D018895:Microsatellite Repeats; D011123:Polyploidy
D005819:Genetic Markers; D004247:DNA