[An experimental study on c-myc gene mRNA expression of endothelial cell induced by bFGF].
OBJECTIVE
To investigate the molecular biological mechanism of endothelial cell proliferation induced by bFGF.


METHODS
Cultured rat myocardial microvascular endothelial cells were stimulated with bFGF of various concentrations. By northern blot analysis, the levels of c-myc mRNA expression were detected.


RESULTS
The expression of c-myc mRNA in the bFGF-treated groups increased (P < 0.05) with a dose- and stimulating time-dependent manner. The c-myc mRNA expression reached to a peak level at 2 hours.


CONCLUSIONS
C-myc expression may be an important component in controlling the transit of cells through the cell cycle.
Zhou
Zhigang
Z
Department of Plastic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
He
Qinglian
Q
Lin
Zihao
Z
Yang
Songlin
S
Zhu
Xiaohai
X
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D016259:Genes, myc / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2