title: Identification of antigenic peptide recognized by the anti-JL1 leukemia-specific monoclonal antibody from combinatorial peptide phage display libraries.
abstract: OBJECTIVE
  In the present study an antigen-mimetic peptide of the anti-JL1 leukemia-specific monoclonal antibody (mAb) was identified and characterized.
  
  
  METHODS
  From combinatorial peptide phage display libraries displaying the random linear heptapeptides and dodecapeptides, we selected clones with affinity to anti-JL1 mAb through repeated rounds of panning on a mAb-coated ELISA plate. The antigenicity and immunogenicity of the peptide epitopes were then studied using chemically synthesized peptides.
  
  
  RESULTS
  The selected clones had the LXPSIP consensus sequence. Two synthetic peptides LPPSIPFGLTVGGGGS and LLPSIPNQAYLGGGGS specifically reacted with anti-JL1 mAb in ELISA. These two peptides were found to inhibit the interaction between anti-JL1 mAb and JL1 antigen-positive Molt-4 cells. Although the immune sera raised against the keyhole limpet hemocyanin-conjugated peptides failed to react with Molt-4 cells, it showed strong reactivity to the peptide epitope. However, one mAb raised by peptide immunization successfully bound to Molt-4 cells.
  
  
  CONCLUSIONS
  An epitope-mimetic peptide of anti-JL1 mAb was found using combinatorial peptide phage display libraries. It induced strong humoral response against itself, but only a limited fraction of this humoral response was cross-reactive with the original JL1 antigen.
authors.affiliation: National Cancer Center, Ilsan-gu, Goyang, Gyeonggi, Korea.
authors.forename: Junho
authors.initials: J
authors.lastname: Chung
authors.forename: Sojung
authors.initials: S
authors.lastname: Park
authors.forename: Dongjo
authors.initials: D
authors.lastname: Kim
authors.forename: Junghyo
authors.initials: J
authors.lastname: Rhim
authors.forename: Ikjung
authors.initials: I
authors.lastname: Kim
authors.forename: Inhak
authors.initials: I
authors.lastname: Choi
authors.forename: Kyesook
authors.initials: K
authors.lastname: Yi
authors.forename: Sungho
authors.initials: S
authors.lastname: Ryu
authors.forename: Pannghill
authors.initials: P
authors.lastname: Suh
authors.forename: Doohyun
authors.initials: D
authors.lastname: Chung
authors.forename: Youngmee
authors.initials: Y
authors.lastname: Bae
authors.forename: Youngkee
authors.initials: Y
authors.lastname: Shin
authors.forename: Sunghoe
authors.initials: S
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000936:Antigen-Antibody Complex; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry* / Q000276:immunology; D001666:Binding Sites, Antibody; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000235:genetics / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007938:Leukemia / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D019151:Peptide Library; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000945:Antigens, Differentiation, T-Lymphocyte; D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; C114952:JL1 antigen; D010446:Peptide Fragments; D019151:Peptide Library