title: Analysis and quantitative determination of group B saponins in processed soybean products.
abstract: Pure analytical standards for the major saponins present in processed soy products, the group B saponins (soyasaponins I, II, III and IV), were isolated in mg quantities by a combination of processing, precipitation/re-solubilisation, TLC and preparative HPLC. These standards were determined to be pure by LC-ESI/MS analysis and NMR. The standards were used to perfect a facile analytical HPLC method using spectrometric detection to determine the percent composition of the group B soyasaponins in various products from processing of soybean.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research Center, 1815 North University Street, Peoria, Illinois, USA. berhowma@ncaur.usda.gov
authors.forename: Mark A
authors.initials: MA
authors.lastname: Berhow
authors.forename: Charles L
authors.initials: CL
authors.lastname: Cantrell
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Duval
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Dobbins
authors.forename: Jonathan
authors.initials: J
authors.lastname: Maynes
authors.forename: Steven F
authors.initials: SF
authors.lastname: Vaughn
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D005511:Food Handling; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification; D013025:Glycine max / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D012503:Saponins; C407096:soyasapogenol B; C116604:soyasapogenol A; C056684:soyasaponin I; C072357:soyasaponin III; C106126:soyasaponin II; D009828:Oleanolic Acid