Molecular cloning, mRNA expression and chromosomal localization of mouse angiotensin-converting enzyme-related carboxypeptidase (mACE2).
We isolated two mouse cDNA clones which show significant similarities with human angiotensin-converting enzyme-related carboxypeptidase (ACE2). The cDNAs were 2746 and 1995 bp in length and seemed to arise from the same gene by alternative splicing. The longer cDNA encoded a 798-amino acid protein containing the sequence motif conserved among zinc metallopeptidases. Mouse ACE2 showed 83% identity with human ACE2. Northern blot analysis revealed that 2.8- and 2.0-kb transcripts were expressed mainly in the kidney and the lungs. The mouse ACE2 gene was mapped to chromosome X 70.5 cM.
Komatsu
Takami
T
Laboratory of Genome Structure Analysis, Human Genome Center, Institute of Medical Science, University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Suzuki
Yutaka
Y
Imai
Junichi
J
Sugano
Sumio
S
Hida
Munetomo
M
Tanigami
Akira
A
Muroi
Sawako
S
Yamada
Yoshitsugu
Y
Hanaoka
Kazuo
K
D000595:Amino Acid Sequence; D000085962:Angiotensin-Converting Enzyme 2; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002268:Carboxypeptidases / Q000096:biosynthesis / Q000235:genetics*; D002874:Chromosome Mapping; D041321:Chromosomes, Human, X; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007668:Kidney; D008168:Lung; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D007703:Peptidyl-Dipeptidase A; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D002268:Carboxypeptidases; D007703:Peptidyl-Dipeptidase A; C000705307:ACE2 protein, human; C000705328:Ace2 protein, mouse; D000085962:Angiotensin-Converting Enzyme 2