Characterization of the poly(A) binding proteins expressed during oogenesis and early development of Xenopus laevis.
During vertebrate oogenesis and early embryogenesis, gene expression is governed mainly by translational control. The recruitment of Poly(A) Binding Protein (PABP) during poly(A) tail lengthening appears to be the key to translational activation during this period of development in Xenopus laevis. We showed that PABP1 and ePABP proteins are both present during oogenesis and early development. We selected ePABP as an eRF3 binding protein in a two-hybrid screening of a X. laevis cDNA library and demonstrated that this protein is associated with translational complexes. It can complement essential functions of the yeast homologue Pab1p. We discuss specific expression patterns of the finely tuned PABP1 and ePABP proteins.
Cosson
Bertrand
B
Université de Rennes 1, CNRS UMR 6061, IFR 97, 2, avenue Pr Léon Bernard, 35043 Rennes cedex, France.
Couturier
Anne
A
Le Guellec
René
R
Moreau
Jacques
J
Chabelskaya
Svetlana
S
Zhouravleva
Galina
G
Philippe
Michel
M
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D039603:Eukaryotic Initiation Factor-4G / Q000235:genetics / Q000378:metabolism; D039642:Eukaryotic Initiation Factors / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D046911:Macromolecular Substances; D009865:Oocytes / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D009866:Oogenesis / Q000235:genetics*; D010454:Peptide Termination Factors / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D039122:Poly(A)-Binding Protein I / Q000235:genetics / Q000378:metabolism; D039102:Poly(A)-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics / Q000378:metabolism
D039603:Eukaryotic Initiation Factor-4G; D039642:Eukaryotic Initiation Factors; D046911:Macromolecular Substances; C424905:PABPC4 protein, Xenopus; D010454:Peptide Termination Factors; D039122:Poly(A)-Binding Protein I; D039102:Poly(A)-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D029867:Xenopus Proteins; C450100:pab1 protein, S cerevisiae; C087971:peptide-chain-release factor 3