title: The automated radiosynthesis of [18F]FP-TZTP.
abstract: [(18)F]FP-TZTP (3-(3-(3-[(18)F]fluoropropylthio)-1,2,5-thiadiazol-4-yl)-1,2,5,6-tetrahydro-1-methylpyridine) is a muscarinic ligand that displays in vivo selectivity for the M2 subtype. We have developed a one-step radiosynthesis of [(18)F]FP-TZTP that can be conducted with an automated synthesis unit. A number of hardware and software modifications to a Nuclear Interface C-11 Methylation System provided the equipment for the automated radiosynthesis. The manual synthesis produced [(18)F]FP-TZTP in a radiochemical yield of 23.4% +/- 4.3% (EOS, n = 69) with a specific activity of 4377 +/- 2011 mCi/micromol (EOB, n = 100). The automated synthesis unit provided the product in a radiochemical yield of 18.8% +/- 2.4% (EOS, n = 25) with a specific activity of 4112 +/- 2572 mCi/micromol (EOB, n = 25).
authors.affiliation: Positron Emission Tomography Department, Clinical Center, National Institutes of Health, 10/1C401, 10 Center Drive, Bethesda, MD 20892, USA. dk7k@nih.gov
authors.forename: Dale O
authors.initials: DO
authors.lastname: Kiesewetter
authors.forename: Bik-kee
authors.initials: BK
authors.lastname: Vuong
authors.forename: Michael A
authors.initials: MA
authors.lastname: Channing
mesh_terms: D004867:Equipment Design; D005462:Fluorine Radioisotopes / Q000737:chemistry*; D007553:Isotope Labeling / Q000295:instrumentation* / Q000379:methods; D011725:Pyridines / Q000138:chemical synthesis*; D011786:Quality Control; D019275:Radiopharmaceuticals / Q000138:chemical synthesis*; D012371:Robotics / Q000295:instrumentation*; D013844:Thiazoles / Q000138:chemical synthesis*; D014055:Tomography, Emission-Computed / Q000379:methods
chemical_list: D005462:Fluorine Radioisotopes; D011725:Pyridines; D019275:Radiopharmaceuticals; D013844:Thiazoles; C115177:FP-TZTP