Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set.
A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ~120,000 cDNA clones, which were initially re-arrayed into a set of ~11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.
VanBuren
Vincent
V
Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging (NIA), National Institutes of Health, Baltimore, Maryland, 20892, USA.
Piao
Yulan
Y
Dudekula
Dawood B
DB
Qian
Yong
Y
Carter
Mark G
MG
Martin
Patrick R
PR
Stagg
Carole A
CA
Bassey
Uwem C
UC
Aiba
Kazuhiro
K
Hamatani
Toshio
T
Kargul
George J
GJ
Luo
Amber G
AG
Kelso
Janet
J
Hide
Winston
W
Ko
Minoru S H
MS
D000818:Animals; D000831:Animals, Newborn / Q000235:genetics*; D001483:Base Sequence / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D019295:Computational Biology / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics*; D030541:Databases, Genetic; D004622:Embryo, Mammalian / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D015723:Gene Library; D005796:Genes / Q000235:genetics; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary