title: [Preimplantation genetic diagnosis].
abstract: OBJECTIVE
  To evaluate the clinical effectiveness of preimplantation genetic diagnosis (PGD) for the couples having a higher risk of genetic diseases with fluorescence in situ hybridization (FISH).
  
  
  METHODS
  Ovarian hyperstimulation, oocyte retrieval and in vitro fertilization were performed in 4 cases. Blastomere biopsy were performed from the embryos at the 6 - 8 cell stage. Genetic diagnosis was identified by single blastomere FISH. The female embryos or normal embryos were transferred into uterus.
  
  
  RESULTS
  Twelve embryos were available for embryo biopsy. Twenty cells were collected. Seventeen nuclei were found after fixation. Hybridization signals of 11 embryos were clear and 8 embryos were transferred into uterus. One clinical pregnancy was achieved, and 1 normal female infant was delivered on september 14, 2001.
  
  
  CONCLUSIONS
  PGD is very valuable for avoiding selective abortion and birth of affected child of the couples having a higher risk of genetic diseases by detecting embryo sex and chromosome number using FISH.
authors.affiliation: Reproductive Medical Research Center, Shandong Institute for Family Planning, Jinan 250002, China.
authors.forename: Xiangge
authors.initials: X
authors.lastname: Meng
authors.forename: Lihong
authors.initials: L
authors.lastname: Zhang
authors.forename: Yunling
authors.initials: Y
authors.lastname: Dong
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Kehua
authors.initials: K
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
authors.forename: Meixin
authors.initials: M
authors.lastname: Zhnag
authors.forename: Yumei
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000328:Adult; D001706:Biopsy; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D011247:Pregnancy; D019836:Preimplantation Diagnosis