Curcumin induces caspase-3-independent apoptosis in human multidrug-resistant cells.
Curcumin, the major component of the spice turmeric, used in 50- micro M concentration, induced cell death in multidrug-resistant CEM(P-gp4) and LoVo(P-gp4) cells as well as in their sensitive counterparts as assessed by TUNEL method and morphological observation. In all cells induced to undergo cell death with curcumin, there was no caspase-3 activation because only the unprocessed form of caspase-3 was observed using immunoblotting.
Piwocka
Katarzyna
K
Laboratory of Molecular Bases of Aging, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland. kapi@nencki.gov.pl
Bielak-Mijewska
Anna
A
Sikora
Ewa
E
D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D003474:Curcumin / Q000494:pharmacology*; D018432:Drug Resistance, Multiple / Q000502:physiology*; D006801:Humans; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; D003474:Curcumin