title: Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cDNA fragments.
authors.affiliation: Institute of Dentistry, University of Oulu, Finland.
authors.forename: Jyrki T
authors.initials: JT
authors.lastname: Aatsinki
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D007202:Indicators and Reagents; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D007202:Indicators and Reagents; D012313:RNA