title: Intestinal alkaline phosphatase release is not associated with chylomicron formation.
abstract: Intestinal alkaline phosphatase (IAP) is one of the major sources of alkaline phosphatase in circulation. It is secreted into the intestinal lumen, serum, and lymph. After the ingestion of lipid, lymphatic alkaline phosphatase secretion increases significantly. We have found that the nonabsorbable fat olestra is unable to stimulate lymphatic alkaline phosphatase secretion. We also found that the hydrophobic surfactant Pluronic L-81, which blocks chylomicron formation, fails to inhibit this increase in lymphatic alkaline phosphatase secretion. These results suggest that it is the lipid uptake into the mucosa and/or reesterification to form triacylglycerols, but not the formation of chylomicrons, that is necessary for the stimulation of the secretion of alkaline phosphatase into the lymph.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0529, USA.
authors.forename: Andromeda M
authors.initials: AM
authors.lastname: Nauli
authors.forename: Shuqin
authors.initials: S
authors.lastname: Zheng
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: Ronggui
authors.initials: R
authors.lastname: Li
authors.forename: Ronald
authors.initials: R
authors.lastname: Jandacek
authors.forename: Patrick
authors.initials: P
authors.lastname: Tso
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002914:Chylomicrons / Q000096:biosynthesis*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004951:Esterification; D019358:Fat Substitutes / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology; D006056:Golgi Apparatus / Q000378:metabolism; D007413:Intestinal Mucosa / Q000201:enzymology*; D008196:Lymph / Q000201:enzymology; D008297:Male; D020442:Poloxamer / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013395:Sucrose / Q000031:analogs & derivatives* / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology; D014280:Triglycerides / Q000378:metabolism
chemical_list: D002914:Chylomicrons; D019358:Fat Substitutes; D005227:Fatty Acids; D013501:Surface-Active Agents; D014280:Triglycerides; D020442:Poloxamer; D013395:Sucrose; C013692:sucrose polyester; D000469:Alkaline Phosphatase