title: A defined human gastrin sequence stimulates the growth of Helicobacter pylori.
abstract: This study describes the interaction between gastrin and Helicobacter pylori. Human gastrin amino acids 4-17 were found to be the minimal growth-stimulating sequence. Gastrin from other mammals did not stimulate bacterial growth. When human serum was used to stimulate bacterial growth in brucella broth, gastrin was shown to be a necessary and sufficient growth-stimulating factor. Competition for the gastrin effect by pentagastrin and cholecystokinin (CCK-8) resulted in inhibition of bacterial growth. This effect was mediated by the four C-terminal amino acids which are shared by gastrin, CCK-8 and pentagastrin. In conclusion, the interaction between gastrin and H. pylori was shown to be specific, essential, and dependent on a defined gastrin sequence.
authors.affiliation: Infectious Disease Unit, Meir Hospital, Sapir Medical Center, Kfar Saba 44281, Israel. chowersm@post.tau.ac.il
authors.forename: Michal Yael
authors.initials: MY
authors.lastname: Chowers
authors.forename: Nathan
authors.initials: N
authors.lastname: Keller
authors.forename: Simon
authors.initials: S
authors.lastname: Bar-Meir
authors.forename: Yehuda
authors.initials: Y
authors.lastname: Chowers
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000145:classification / Q000378:metabolism; D005755:Gastrins / Q000737:chemistry* / Q000494:pharmacology*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development*; D006801:Humans; D008969:Molecular Sequence Data; D010418:Pentagastrin / Q000378:metabolism
chemical_list: D000596:Amino Acids; D005755:Gastrins; C472485:gastrin (4-17); D010418:Pentagastrin