In vitro autoreactivity against skin and synovial cells in patients with juvenile idiopathic and rheumatoid arthritis.
In the present study we compared specific lysis of various autologous target cells in patients with juvenile idiopathic arthritis JIA; n = 8) or rheumatoid arthritis RA; n = 17) with those of healthy controls (n = 15). (51)Cr-release cytotoxic assay with autologous peripheral blood mononuclear cells as effector cells was used. When compared with controls, effector cells of patients with JIA or RA were found to lyse significantly autologous synovial cells (p < 0.0005) and epidermal keratinocytes (p < 0.0005), however, no difference was found for autologous dermal fibroblasts.
Stechova
K
K
2nd Department of Paediatrics, University Hospital Motol, Prague, Czech Republic. katerina.stechova@lfmotol.cuni.cz
Vavrincova
P
P
Chudoba
D
D
Frantlova
M
M
Reitzova
H
H
Sosna
A
A
Zimak
J
J
Lednicky
L
L
Dickinson
A M
AM
Hromadnikova
I
I
D000293:Adolescent; D000328:Adult; D001171:Arthritis, Juvenile / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000276:immunology; D015551:Autoimmunity / Q000276:immunology*; D016923:Cell Death / Q000276:immunology; D002478:Cells, Cultured; D002648:Child; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D005260:Female; D005347:Fibroblasts / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000276:immunology; D013583:Synovial Membrane / Q000276:immunology*