Alterations in skeletal protein, distribution of PKCalpha, and level of phospholipids in erythrocyte membranes of women with primary breast cancer.
The aim of our work was to study the influence of primary breast cancer on mature erythrocyte membranes. Blood was sampled from 29 women with primary breast cancer, aged 35-86 years, in different stages of clinical progression of the disease. In red blood cell membranes an increase of phosphatidylinositol-4-phosphate and phosphatidylinositol-4,5-diphosphate levels was observed. These changes were accompanied by a decrease in phospholipase C activity. Simultaneously, a significant decrease in concentration of phosphatidylserine, sphingomyelin, and phosphatidylinositol was found. Quantitative protein evaluation showed an increase in band 4.1 protein content with no changes in the level of constitutive PKCalpha responsible for the phosphorylation of this protein and its affinity to glycophorine C. In parallel a greater increase of PKCalpha translocation after PMA treatment compared to controls was observed. Possible oxidative damage of erythrocyte membranes indicated by an increase in malonyldialdehyde level and decrease in SH-group content as well as by an increase in the w/ ratio was documented. From the results it is concluded that primary breast cancer seems to affect the membranes of mature erythrocytes.
Kaczmarek
Jolanta
J
Department of Clinical Chemistry, Karol Marcinkowski University of Medical Sciences in Poznań, Poznań, Poland.
Thieleman
Anna
A
Kopczyński
Zygmunt
Z
Goslar
Janina
J
Hoffmann
Stanisław Kazimierz
SK
Rybczyńska
Maria
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000097:blood* / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000032:analysis*; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000473:pathology*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008565:Membrane Proteins / Q000032:analysis; D008875:Middle Aged; D009479:Neuropeptides; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000032:analysis*; D011493:Protein Kinase C / Q000378:metabolism*; D051571:Protein Kinase C-alpha; D015398:Signal Transduction; D013438:Sulfhydryl Compounds / Q000032:analysis; D013755:Tetradecanoylphorbol Acetate
D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D009479:Neuropeptides; D010743:Phospholipids; D013438:Sulfhydryl Compounds; C028841:erythrocyte membrane band 4.1 protein; C120557:erythrocyte membrane protein band 4.1-like 1; C496628:PRKCA protein, human; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D013755:Tetradecanoylphorbol Acetate