A putative novel nuclear-encoded subunit of the cytochrome c oxidase complex in trypanosomatids.
A relatively large nuclear-encoded polypeptide, designated trCOIV, is found in the cytochrome c oxidase (CO) complex of trypanosomatids. In order to determine if this polypeptide represents a bona fide subunit of the complex, we have characterized the cDNA and the gene for this polypeptide in Leishmania tarentolae. Its nuclear gene has no sequence similarity to mammalian COIV. The trCOIV preprotein has a long mitochondrial targeting sequence of 31 residues. The mature polypeptide cofractionates with kinetoplast-mitochondria and its preferential mitochondrial localization was confirmed by immunofluorescence and immunoelectron microscopy. Based on the hydropathy plot analysis, the protein lacks pronounced transmembrane domains and likely occupies a peripheral position within the CO complex. The corresponding genes are also present in the sequenced portions of the Trypanosoma cruzi, Trypanosoma brucei and Leishmania major genomes, and the same polypeptide is found in cytochrome oxidase isolated from procyclic T. brucei and promastigote Leishmania mexicana amazonensis. However, the trCOIV gene, the mRNA and the polypeptide could not be detected in a respiration-deficient trypanosomatid Phytomonas serpens.
Maslov
Dmitri A
DA
Department of Biology, University of California, Riverside, CA 92521, USA. maslov@ucrac1.ucr.edu
Zíková
Alena
A
Kyselová
Iveta
I
Lukes
Julius
J
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000201:enzymology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics*; D017125:Genes, Protozoan / Q000235:genetics; D007150:Immunohistochemistry; D046911:Macromolecular Substances; D008928:Mitochondria / Q000502:physiology / Q000648:ultrastructure; D008969:Molecular Sequence Data; D021122:Protein Subunits; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D014351:Trypanosomatina / Q000145:classification / Q000201:enzymology / Q000235:genetics*
D018076:DNA, Complementary; D046911:Macromolecular Substances; D021122:Protein Subunits; D012333:RNA, Messenger; D003576:Electron Transport Complex IV