title: Methicillin resistant Staphylococcus aureus: a multicentre study.
abstract: OBJECTIVE
  To determine the frequency of Methicillin resistance Staphylococcus aureus infection in major cities of Pakistan.
  
  
  METHODS
  Various laboratories of the country with one as the central Laboratory.
  
  
  METHODS
  Seven hundred and ninety two consecutive clinical isolates of Staphylococcus aureus were collected from 8 laboratories all over Pakistan i.e. Karachi, Peshawar, Lahore, Sukkhur, Islamabad, Quetta, and Mirpur, Azad Kashmir. Antibiotic sensitivity was done by Kirby Bauer disc diffusion method and MIC of Vancomycin was determined by 'E' test.
  
  
  RESULTS
  Forty two percent of the isolates were found to be Methicillin resistant staphylococcus aureus (MRSA) while no Vancomycin resistance was encountered.
  
  
  CONCLUSIONS
  Methicillin resistant Staphylococcus aureus (MRSA) are seen in the local population with frequencies varying between 2-61% highest incidence is seen in the major cities of the country. Fortunately no Vancomycin resistant Staphylococcus has been isolated from any of the major cities.
authors.affiliation: Mid-East Medical Center, Karachi.
authors.forename: S
authors.initials: S
authors.lastname: Hafiz
authors.forename: A N
authors.initials: AN
authors.lastname: Hafiz
authors.forename: L
authors.initials: L
authors.lastname: Ali
authors.forename: A S
authors.initials: AS
authors.lastname: Chughtai
authors.forename: B
authors.initials: B
authors.lastname: Memon
authors.forename: A
authors.initials: A
authors.lastname: Ahmed
authors.forename: S
authors.initials: S
authors.lastname: Hussain
authors.forename: G
authors.initials: G
authors.lastname: Sarwar
authors.forename: T
authors.initials: T
authors.lastname: Mughal
authors.forename: S J
authors.initials: SJ
authors.lastname: Siddiqui
authors.forename: A
authors.initials: A
authors.lastname: Awan
authors.forename: K
authors.initials: K
authors.lastname: Zaki
authors.forename: A
authors.initials: A
authors.lastname: Fareed
mesh_terms: D003906:Developing Countries; D006801:Humans; D015994:Incidence; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D010154:Pakistan / Q000453:epidemiology; D011446:Prospective Studies; D018570:Risk Assessment; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000453:epidemiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification; D014640:Vancomycin / Q000494:pharmacology*
chemical_list: D014640:Vancomycin