Localisation of aphidicolin-induced break points in Holstein-Friesian cattle (Bos taurus) using RBG-banding.
Fragile sites (FS) seem to play a role in genome instability and may be involved in karyotype evolution and chromosome aberrations. The majority of common fragile sites are induced by aphidicolin. Aphidicolin was used at two different concentrations (0.15 and 0.30 microM) to study the occurrence of FS in the cattle karyotype. In this paper, a map of aphidicolin induced break points and fragile sites in cattle chromosomes was constructed. The statistical analysis indicated that any band with three or more breaks was significantly damaged (P<0.05). According to this result, 30 of the 72 different break points observed were scored as fragile sites. The Pearson correlation test showed a positive association between chromosome length and the number of fragile sites (r=0.54). On the contrary, 21 FS were identified on negative R bands while 9 FS were located on positive R bands.
Rodriguez
Viviana
V
Department of Cell and Molecular Biology, Genetic Section, Laboratory of Genetics Analysis in Domestic Animals, Faculty of Veterinary, UDELAR, A. Lasplaces 1550 CP 11600, Montevideo, Uruguay.
Llambí
Silvia
S
Postiglioni
Alicia
A
Guevara
Karina
K
Rincón
Gonzalo
G
Fernández
Gabriel
G
Mernies
Beatriz
B
Arruga
María Victoria
MV
D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology*; D002417:Cattle; D002871:Chromosome Banding; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D002874:Chromosome Mapping; D002875:Chromosomes / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D016590:Aphidicolin