The effect of liposomes on thermotropic membrane phase transitions of bovine spermatozoa and oocytes: implications for reducing chilling sensitivity.
We have examined the effects of combinations between egg-phosphatidylcholine (EPC) or dipalmitoylphosphatidylcholine (DPPC) liposomes with either bovine spermatozoa or oocytes on cellular chilling sensitivity, lipid phase transition temperature (T(m)), and the ability of the oocytes to develop to the blastocyst stage. Spermatozoa and oocytes were exposed to EPC and DPPC liposomes at various temperatures (spermatozoa: 4, 12, 16, and 25 degrees C; oocytes: 4, 16, and 32 degrees C). The membrane integrity of the spermatozoa-control group decreased significantly following exposure to 16 or 12 degrees C, compared to ambient temperature (25 degrees C). In contrast, the EPC-sperm group had a greater resistance to chilling at each temperature and showed a decline in membrane integrity only at the lowest temperatures investigated. However, the DPPC-sperm group was injured significantly at all temperatures tested. Similar to the sperm, oocytes from the control group that were exposed to 16 degrees C were injured more severely than oocytes that were electrofused with EPC or DPPC liposomes. The membrane integrity of the oocytes at 16 degrees C that were electrofused with either EPC or DPPC liposomes was approximately the same as the control group held at 32 degrees C (normalized to 100%), compared to 46% in the control group at 16 degrees C (P<0.01). The transition temperatures of the sperm and oocyte membranes revealed different T(m) for the different liposome treatments. All groups had a significantly higher cleavage rate, as well as increased blastocyst formation when oocytes were exposed to temperatures above or below their T(m). We suggest that the T(m) of spermatozoa or oocytes can be changed by spontaneous association or electrofusion of liposomes with cellular membranes and, consequently, the chilling sensitivity can be altered. The resulting possibility is that embryo development after cryopreservation could be improved with such a method.
Zeron
Y
Y
Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel.
Tomczak
M
M
Crowe
J
J
Arav
A
A
D015060:1,2-Dipalmitoylphosphatidylcholine; D000818:Animals; D002417:Cattle; D003080:Cold Temperature; D015925:Cryopreservation; D005314:Embryonic and Fetal Development; D005260:Female; D066298:In Vitro Techniques; D008081:Liposomes; D008297:Male; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism*; D010713:Phosphatidylcholines; D013094:Spermatozoa / Q000378:metabolism*; D013816:Thermodynamics
D008081:Liposomes; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D015060:1,2-Dipalmitoylphosphatidylcholine