Gas chromatographic-mass spectrometric detection of 2- and 3-hydroxy fatty acids as methyl esters from soil, sediment and biofilm.
Hydroxy fatty acids (OH-FAs) can be used in the characterization of microbial communities, especially Gram-negative bacteria. We prepared methyl esters of 2- and 3-OH-FAs from the lipid extraction residue of soil, sediment, and biofilm samples without further purification or derivatization of hydroxyl groups. OH-FA methyl esters were analyzed using a gas chromatograph equipped with a mass selective detector (GC-MS). The ions followed in MS were m/z 103 for 3-OH-FAs and m/z 90 and M-59 for 2-OH-FAs. The rapid determination of 3- and 2-OH-FAs concomitantly with phospholipid fatty acids provided more detailed information on the microbial communities present in soil, sediment, and drinking water biofilm.
Keinänen
M M
MM
Department of Environmental Health, National Public Health Institute, P.O. Box 95, FIN-70701, Kuopio, Finland. minna.keinanen@ktl.fi
Korhonen
L K
LK
Martikainen
P J
PJ
Vartiainen
T
T
Miettinen
I T
IT
Lehtola
M J
MJ
Nenonen
K
K
Pajunen
H
H
Kontro
M H
MH
D018441:Biofilms; D004952:Esters; D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D019015:Geologic Sediments / Q000737:chemistry*; D012987:Soil / Q000032:analysis*
D004952:Esters; D005227:Fatty Acids; D012987:Soil