Screening for novel enzymes for biocatalytic processes: accessing the metagenome as a resource of novel functional sequence space.
Historically, biotechnology has missed up to 99% of existing microbial resources by using traditional screening techniques. Strategies of directly cloning 'environmental DNA' comprising the genetic blueprints of entire microbial consortia (the so-called 'metagenome') provide molecular sequence space that along with ingenious in vitro evolution technologies will act synergistically to bring a maximum of available sequence-space into biocatalytic application.
Lorenz
Patrick
P
BRAIN Aktiengesellschaft, Darmstaedter Strasse 34, D-64673, Zwingenberg, Germany. pl@brain.biotech.de
Liebeton
Klaus
K
Niehaus
Frank
F
Eck
Jürgen
J
D001419:Bacteria / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D002384:Catalysis; D003001:Cloning, Molecular; D017753:Ecosystem; D004789:Enzyme Activation; D004798:Enzymes / Q000302:isolation & purification*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D016680:Genome, Bacterial; D015698:Genomic Library; D011994:Recombinant Proteins / Q000302:isolation & purification
D004798:Enzymes; D011994:Recombinant Proteins