title: Passive transfer of serum antibodies induced by BBG2Na, a subunit vaccine, in the elderly protects SCID mouse lungs against respiratory syncytial virus challenge.
abstract: Respiratory syncytial virus (RSV) is responsible for severe low respiratory tract infections in young infants and the elderly. To investigate whether BBG2Na, a recombinant subunit vaccine comprising aa 130-230 of the RSV G protein, induced protective Abs in subjects over 60 years during phase II clinical trial, pre- and postimmunization sera of individuals immunized with BBG2Na or placebo were transferred into SCID mice before RSV challenge. These sera dose-dependently reduced lung RSV titers. However at some points of serial dilutions, postimmunization sera of BBG2Na-immunized subjects only were significantly more efficient than the corresponding preimmunization sera, in agreement with the induction of an increased Ab response against multiple epitopes on RSV-A G protein. Thus, BBG2Na is immunogenic in the elderly and confers passive protection in mice after serum transfer. To our knowledge, this is the first description of protective Abs induced by a subunit vaccine in human.
authors.affiliation: Centre d'Immunologie Pierre Fabre, 5 Av. Napoléon III, F74 164, Saint-Julien-en-Genevois, France. helene.plotnicky.gilquin@pierre-fabre.com
authors.forename: Hélène
authors.initials: H
authors.lastname: Plotnicky-Gilquin
authors.forename: Dominique
authors.initials: D
authors.lastname: Cyblat-Chanal
authors.forename: Liliane
authors.initials: L
authors.lastname: Goetsch
authors.forename: Christine
authors.initials: C
authors.lastname: Lacheny
authors.forename: Christine
authors.initials: C
authors.lastname: Libon
authors.forename: Thierry
authors.initials: T
authors.lastname: Champion
authors.forename: Alain
authors.initials: A
authors.lastname: Beck
authors.forename: Hélène
authors.initials: H
authors.lastname: Pasche
authors.forename: Thien N
authors.initials: TN
authors.lastname: Nguyen
authors.forename: Jean Yves
authors.initials: JY
authors.lastname: Bonnefoy
authors.forename: Nancy
authors.initials: N
authors.lastname: Bouveret-le-Cam
authors.forename: Nathalie
authors.initials: N
authors.lastname: Corvaïa
mesh_terms: D000281:Administration, Intranasal; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000914:Antibodies, Viral / Q000008:administration & dosage* / Q000096:biosynthesis; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D015707:HN Protein / Q000235:genetics; D006801:Humans; D007116:Immunization, Passive; D008168:Lung / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D008875:Middle Aged; D011995:Recombination, Genetic; D018357:Respiratory Syncytial Virus Infections / Q000517:prevention & control* / Q000821:virology; D022261:Respiratory Syncytial Virus Vaccines / Q000008:administration & dosage / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology; D014759:Viral Envelope Proteins
chemical_list: D000914:Antibodies, Viral; D015707:HN Protein; D022261:Respiratory Syncytial Virus Vaccines; D022223:Vaccines, Subunit; D014759:Viral Envelope Proteins; C116204:attachment protein G