Organization in lipid membranes containing cholesterol.
A fundamental attribute of raft formation in cell membranes is lateral separation of lipids into coexisting liquid phases. Using fluorescence microscopy, we observe spontaneous lateral separation in free-floating giant unilamellar vesicles. We record coexisting liquid domains over a range of composition and temperature significantly wider than previously reported. Furthermore, we establish correlations between miscibility in bilayers and in monolayers. For example, the same lipid mixtures that produce liquid domains in bilayer membranes produce two upper miscibility critical points in the phase diagrams of monolayers.
Veatch
Sarah L
SL
Department of Chemistry, University of Washington, Seattle, Washington 98195-1700, USA.
Keller
Sarah L
SL
D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D004083:Cholestanol / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry* / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry; D008856:Microscopy, Fluorescence; D010713:Phosphatidylcholines / Q000737:chemistry*
D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D004083:Cholestanol; D002784:Cholesterol