The function of the nuclear matrix attachment region of silkworm rDNA as an autonomously replicating sequence in plasmid and chromosomal replication origin in yeast.
Nuclear matrix attachment regions (MARs) play a crucial role in chromatin architecture, gene expression, and DNA replication. Although it is well known that yeast autonomously replicating sequences (ARSs) bind nuclear matrix and MARs also function as ARS elements in yeast, whether a heterologous MAR or ARS element acts as a replication origin in the chromosome has not been elucidated. We previously identified a MAR (rMAR) located in the nontranscribed spacer (NTS) of silkworm Attacus ricini rDNA. We report here that this rMAR contains 10 copies of ARS consensus sequence (ACS) and several DNA unwinding regions. The rMAR employs ARS activity in yeast and a rARS element locates in the 3(') region of the rMAR. Furthermore, we have also revealed that either the rMAR or the rARS element functions as a replication origin in the chromosome. Our results provide the first direct evidence to demonstrate that heterologous rMAR and rARS display chromosomal origin activity, suggesting that the chromosome structure and replication origin of rDNA reserve some common features during evolution.
Chen
Ying
Y
The Institute of Molecular Biology, The University of Hong Kong, Hong Kong.
Zhao
Mujun
M
Li
Zai-Ping
ZP
He
Ming-Liang
ML
D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D015825:Chromosomes, Fungal; D016384:Consensus Sequence; D004261:DNA Replication; D004275:DNA, Ribosomal / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D015530:Nuclear Matrix / Q000378:metabolism; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D018741:Replication Origin; D015003:Yeasts / Q000235:genetics
D004275:DNA, Ribosomal