title: Biosynthesis of aminoglycoside antibiotics: cloning, expression and characterisation of an aminotransferase involved in the pathway to 2-deoxystreptamine.
abstract: The gene btrR from Bacillus circulans has been cloned and expressed and shown to produce a protein which catalyses the transamination of 2-deoxy-scyllo-inosose to give 2-deoxy-scyllo-inosamine, an intermediate in the biosynthesis of 2-deoxystreptamine.
authors.affiliation: Cambridge Centre for Molecular Recognition, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK CB2 1EW.
authors.forename: Fanglu
authors.initials: F
authors.lastname: Huang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Li
authors.forename: Jinquan
authors.initials: J
authors.lastname: Yu
authors.forename: Jonathan B
authors.initials: JB
authors.lastname: Spencer
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001407:Bacillus / Q000201:enzymology; D003001:Cloning, Molecular; D016680:Genome, Bacterial; D006595:Hexosamines / Q000096:biosynthesis*; D000637:Transaminases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D006595:Hexosamines; D000637:Transaminases; C007265:2-deoxystreptamine