Association of Bcl-2 with cyclin a/Cdk-2 complex and its effects on Cdk-2 activity.
In the human endometrial carcinoma cell line HEC1B, the overexpression of Bcl-2 leads to a G2/M cell cycle arrest. The experiments presented in this work suggest a direct interaction between the antiapoptotic protein Bcl-2 and the cyclin-dependent kinase Cdk-2 and suggest that such interaction is cell cycle dependent. While further experiments are currently under way in our laboratory to elucidate the significance of Cdk-2/Bcl-2 complexes in PCD and cell cycle regulation, we demonstrate also a specific inhibitory function of Bcl-2 on the activity of the coprecipitated kinase.
Crescenzi
Elvira
E
Dipartimento di Biologia e Patologia Cellulare e Molecolare, University of Naples Federico II, Via S. Pansini, 5, 80131 Naples, Italy, and ASI, Rome, Italy. elviracescenzi@hotmail.com
Sannino
Maria
M
Tonziello
Gilda
G
Palumbo
Giuseppe
G
D042846:CDC2-CDC28 Kinases; D002453:Cell Cycle / Q000502:physiology*; D019925:Cyclin A / Q000378:metabolism*; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D016889:Endometrial Neoplasms; D005260:Female; D006706:Homeostasis / Q000502:physiology; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D019925:Cyclin A; D019253:Proto-Oncogene Proteins c-bcl-2; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases