title: A rapid and simple procedure for direct cloning of PCR products into baculoviruses.
authors.forename: Tamara S
authors.initials: TS
authors.lastname: Gritsun
authors.forename: Michael V
authors.initials: MV
authors.lastname: Mikhailov
authors.forename: Ernest A
authors.initials: EA
authors.lastname: Gould
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D007313:Insecta / Q000821:virology; D008164:Luminescent Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D000077183:Occlusion Body Matrix Proteins; D016133:Polymerase Chain Reaction / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000032:analysis; D014162:Transfection / Q000379:methods; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D008164:Luminescent Proteins; D000077183:Occlusion Body Matrix Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus; D049452:Green Fluorescent Proteins; D001616:beta-Galactosidase