The role of PPAR-gamma/RXR-alpha heterodimers in the regulation of human trophoblast invasion.
PPAR-gamma/RXR heterodimers play a key regulatory role in murine placental development. We report here that PPAR-gamma and RXR-alpha are highly expressed in cultured primary extravillous cytotrophoblasts (EVCT) isolated from human first-trimester placenta. Using this cell culture model, we showed that PPAR-gamma agonists inhibit EVCT invasion, whereas PPAR-gamma or pan-RXR antagonists promoted EVCT invasion by themselves and abolished PPAR-gamma agonist-mediated effect. Together these data underscore an important function of PPAR/RXR heterodimers in the modulation of trophoblast invasion.
Fournier
Thierry
T
INSERM U427, Faculté de Pharmacie, 75006 Paris, France. t.f@pharmacie.univ-paris5.fr
Pavan
Laetitia
L
Tarrade
Anne
A
Schoonjans
Kristina
K
Auwerx
Johan
J
Rochette-Egly
Cecile
C
Evain-Brion
Daniele
D
D000818:Animals; D002478:Cells, Cultured; D019281:Dimerization; D005260:Female; D006801:Humans; D051379:Mice; D010920:Placenta / Q000502:physiology; D011247:Pregnancy; D018160:Receptors, Cytoplasmic and Nuclear / Q000502:physiology*; D018168:Receptors, Retinoic Acid / Q000502:physiology*; D047488:Retinoid X Receptors; D014157:Transcription Factors / Q000502:physiology*; D014327:Trophoblasts / Q000502:physiology*
D018160:Receptors, Cytoplasmic and Nuclear; D018168:Receptors, Retinoic Acid; D047488:Retinoid X Receptors; D014157:Transcription Factors