Universal inheritable barcodes for identifying organisms.
The needs for recognition of novel conventional or transgenic organisms include protection of patented or Identity Preserved lines, detecting transgenics and tracing dispersal. We propose simple 'Biobarcodes' using universal PCR primers to recognize the universal 'nonsense' recognition site of all biobarcodes, followed by a variable nonsense sequence. The proposed sequences are long enough to allow recognition in spite of mutations, have stop codons to prevent coding, and will not self anneal. Sequences of PCR-amplified biobarcodes can be compared to a universal database.
Gressel
Jonathan
J
Plant Sciences, Weizmann Institute of Science, Rehovot, Israel. jonathan.gressel@weizmann.ac.il
Ehrlich
Gal
G
D018744:DNA, Plant / Q000235:genetics; D030541:Databases, Genetic; D010944:Plants / Q000145:classification / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
D018744:DNA, Plant