Helicobacter species ribosomal DNA recovered from the liver tissue of chinese patients with primary hepatocellular carcinoma.
Using polymerase chain reaction (PCR), we examined 16S ribosomal DNA (rDNA) of Helicobacter species in liver tissue specimens obtained from 15 patients with hepatocellular carcinoma. Sixty percent (9 of 15) of these specimens were found to be positive for Helicobacter species. Four 16S rDNA fragments from positive PCR samples were directly sequenced. By sequence comparison, all were found to be 99% identical to the 16S rRNA of Helicobacter pylori.
Fan
Xue-Gong
XG
Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, Hunan, People's Republic of China. xgfan@hotmail.com
Peng
Xiao-Ning
XN
Huang
Yan
Y
Yakoob
Javed
J
Wang
Zhi-Ming
ZM
Chen
Yong-Ping
YP
D000328:Adult; D044466:Asian People; D006528:Carcinoma, Hepatocellular / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis*; D005260:Female; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008113:Liver Neoplasms / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S