Fiber composition and oxidative capacity of hamster skeletal muscle.
The hamster is a valuable biological model for physiological investigation. Despite the obvious importance of the integration of cardiorespiratory and muscular system function, little information is available regarding hamster muscle fiber type and oxidative capacity, both of which are key determinants of muscle function. The purpose of this investigation was to measure immunohistochemically the relative composition and size of muscle fibers composed of types I, IIA, IIX, and IIB fibers in hamster skeletal muscle. The oxidative capacity of each muscle was also assessed by measuring citrate synthase activity. Twenty-eight hindlimb, respiratory, and facial muscles or muscle parts from adult (144-147 g bw) male Syrian golden hamsters (n=3) were dissected bilaterally, weighed, and frozen for immunohistochemical and biochemical analysis. Combining data from all 28 muscles analyzed, type I fibers made up 5% of the muscle mass, type IIA fibers 16%, type IIX fibers 39%, and type IIB fibers 40%. Mean fiber cross-sectional area across muscles was 1665 +/- 328 microm(2) for type I fibers, 1900 +/- 417 microm(2) for type IIA fibers, 3230 +/- 784 microm(2) for type IIX fibers, and 4171 +/- 864 microm(2) for type IIB fibers. Citrate synthase activity was most closely related to the population of type IIA fibers (r=0.68, p<0.0001) and was in the rank order of type IIA > I > IIX > IIB. These data demonstrate that hamster skeletal muscle is predominantly composed of type IIB and IIX fibers.
Mattson
John P
JP
Department of Exercise and Sport Science, University of Utah, Salt Lake City 84112, USA. jmattson@hsc.utah.edu
Miller
Todd A
TA
Poole
David C
DC
Delp
Michael D
MD
D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism; D006224:Cricetinae; D007150:Immunohistochemistry; D008297:Male; D008647:Mesocricetus; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000145:classification / Q000648:ultrastructure*; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure*; D010084:Oxidation-Reduction
D002950:Citrate (si)-Synthase