[Interferon-gamma up-regulates Fas expression and increases Fas-mediated apoptosis in tumor cell lines].
OBJECTIVE
To investigate effect of interferon-gamma (IFN-gamma) on Fas expression and Fas-mediated apoptosis in tumor cell lines.


METHODS
Fas expression was detected by flow cytometry, and the tumor cell apoptosis evaluated by enzyme-linked immunosorbent assay and AnnexinV staining assay.


RESULTS
All the 4 tumor cell lines used in this study, e.g. gastric cancer cell lines Kato-III AGS, lung cancer cell line A549 and laryngeal cancer cell line Hep-2, expressed Fas protein to varied degrees. Fas expression was up-regulated by IFN-gamma (P<0.05), which alone was enough to induce apoptosis in tumor cells and increase the susceptibility of them to anti-Fas antibodies. IFN-gamma induced Fas expression up-regulation and tumor cell apoptosis in a time-dependent manner.


CONCLUSIONS
Fas-mediated apoptosis is one of the mechanisms for IFN-gamma to exercise its anti-tumor effect.
Zheng
Hang
H
Department of Oncology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Luo
Rong-Cheng
RC
Zhang
Luo-Sheng
LS
Mai
Guo-Feng
GF
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D019014:fas Receptor / Q000378:metabolism* / Q000502:physiology
D000970:Antineoplastic Agents; D019014:fas Receptor; D007371:Interferon-gamma