title: The cellular compatibility of five endodontic sealers during the setting period.
abstract: The purpose of this study was to evaluate the cellular compatibility of five endodontic sealers in the first 24 h after mixing. Specimens of N2, Endomethasone, Apexit, AH Plus, and Ketac Endo were extracted with cell culture medium 0, 1, 5, and 24 h after mixing. Eluates were tested for cytotoxicity with immortal 3T3 cells and primary human periodontal ligament fibroblasts using XTT-assays. Data were analyzed for statistically significant differences by means of Dunnett's t tests (p < 0.05). All extracts of N2 completely inhibited cell metabolism (p < 0.05). Similar effects were provoked by the first three eluates of Endomethasone, but the 24-h extract irritated cells significantly less (p < 0.05). Severe cytotoxicity was also observed with all Ketac Endo extracts (p < 0.05). A significant inhibition of mitochondrial activity was induced by the first (3T3) or the first and second eluate (periodontal ligament fibroblasts) of AH Plus (p < 0.05). The subsequent eluates of this sealer and all extracts of Apexit did not reveal any cytotoxic potency.
authors.affiliation: Department of Conservative Dentistry & Periodontology, Medical University Hannover, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Schwarze
authors.forename: I
authors.initials: I
authors.lastname: Fiedler
authors.forename: G
authors.initials: G
authors.lastname: Leyhausen
authors.forename: W
authors.initials: W
authors.lastname: Geurtsen
mesh_terms: D016475:3T3 Cells; D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000633:toxicity; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity*; D002126:Calcium Hydroxide / Q000633:toxicity; D003907:Dexamethasone / Q000633:toxicity; D004338:Drug Combinations; D004853:Epoxy Resins / Q000633:toxicity; D005054:Eugenol / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects; D005557:Formaldehyde / Q000633:toxicity; D005899:Glass Ionomer Cements / Q000633:toxicity; D006801:Humans; D006854:Hydrocortisone; D007202:Indicators and Reagents; D007509:Irritants / Q000633:toxicity; D051379:Mice; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000633:toxicity*; D013223:Statistics as Topic; D013778:Tetrazolium Salts; D013943:Thymol / Q000031:analogs & derivatives* / Q000633:toxicity; D013997:Time Factors; D015034:Zinc Oxide / Q000633:toxicity
chemical_list: D000893:Anti-Inflammatory Agents; C074418:Apexit; D001672:Biocompatible Materials; D004338:Drug Combinations; D004853:Epoxy Resins; D005899:Glass Ionomer Cements; D007202:Indicators and Reagents; D007509:Irritants; C074934:Ketac Endo; D012387:Root Canal Filling Materials; D013778:Tetrazolium Salts; C011244:corticosteroid methanetriol mixture; C534916:epoxy resin-based root canal sealer; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; D005557:Formaldehyde; D013943:Thymol; D005054:Eugenol; D003907:Dexamethasone; C003799:N2 Dental Cement; D002126:Calcium Hydroxide; D015034:Zinc Oxide; D006854:Hydrocortisone