title: [Proliferation and denucleation of K562 cells induced by cytochalasin B].
abstract: OBJECTIVE
  To observe the effect of cytochalasin B on the denucleation of K562 cells.
  
  
  METHODS
  K562 cells were grown in RPMI 1640 medium supplemented with 10% calf serum and 4 microL cytochalasin B (CB). Denucleation was induced in the cultured cells by CB, and the cells were examined by phase contrast microscopy and Giemsa staining respectively.
  
  
  RESULTS
  The denucleation of K562 cells induced by CB was clearly observed, and the cell proliferation was obviously inhibited.
  
  
  CONCLUSIONS
  The denucleation efficiency of K562 cells is positively correlated with CB concentrations and the duration of CB treatment. CB at low doses may inhibit the cell proliferation and at high doses causes cell death.
authors.affiliation: Institute of Molecular Biology, First Military University, Guangzhou 510515, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wei
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Ma
authors.forename: Xiang-Ming
authors.initials: XM
authors.lastname: Mao
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Song
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.forename: Chun-Qiong
authors.initials: CQ
authors.lastname: Feng
authors.forename: Wen-Ling
authors.initials: WL
authors.lastname: Zheng
mesh_terms: D002455:Cell Division / Q000187:drug effects; D003571:Cytochalasin B / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020014:K562 Cells
chemical_list: D003571:Cytochalasin B