title: [Expression of vascular endothelial growth factor C in human breast cancer cell lines MCF-7 and MCF-7/Adr].
abstract: OBJECTIVE
  Vascular endothelial growth factor C (VEGF-C) discovered recently is a new member of vascular endothelial growth factor (VEGF) family. Studies have shown that VEGF-C is one specific regulating factor for lymphatic endothelial cells, and it expresses in a variety of human tumors. In order to explore the role of VEGF-C in the process of human tumor formation and development, the authors designed this study to examine the expressions of VEGF-C mRNA and protein in human breast cancer cell line MCF-7 and drug resistant cell line MCF-7/Adr.
  
  
  METHODS
  According to the VEGF-C gene sequence, A specific oligonucleotide probe labeled with digoxin was designed and constructed; Then in situ hybridization was applied to detect the expression of VEGF-C mRNA in the cultured human breast cancer cell lines MCF-7 and MCF-7/Adr respectively; Finally immunohistochemistry was performed to examine the expression of VEGF-C protein in both cell lines.
  
  
  RESULTS
  Blue positive staining granules for VEGF-C mRNA were observed in the cytoplasm of these cells by in situ hybridization where brown positive staining granules for VEGF-C protein were also observed by immunohistochemistry. While there were no positive staining granules in neither control cells.
  
  
  CONCLUSIONS
  Human breast cancer cell lines MCF-7 and MCF-7/Adr can transcribe VEGF-C mRNA and translate corresponding protein in their cytoplasm.
authors.affiliation: Department of Human Anatomy, Medical School, Shandong University, Jinan 250012, P. R. China. jieyuan_gx@yahoo.com.cn
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Zhi-yu
authors.initials: ZY
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Yu-shun
authors.initials: YS
authors.lastname: Bi
authors.forename: Hua
authors.initials: H
authors.lastname: Tian
mesh_terms: D001943:Breast Neoplasms / Q000737:chemistry / Q000378:metabolism*; D016228:Endothelial Growth Factors / Q000032:analysis / Q000235:genetics*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured; D042582:Vascular Endothelial Growth Factor C
chemical_list: D016228:Endothelial Growth Factors; D012333:RNA, Messenger; D042582:Vascular Endothelial Growth Factor C