Apparent lack of trans-dominant negative effects of various vif mutants on the replication of HIV-1.
The vif gene of human immunodeficiency virus type 1 (HIV-1) is essential for virus growth in non-permissive cells such as H9. To elucidate the mechanism of action of the Vif protein, vif mutants, which show trans-dominant negative effects on the replication of HIV-1, would be useful tools. In this study, a new assay system to identify the mutants of this category was established. For this new system, various reporter clones carrying both mutant and authentic vif sequences were generated. By determining the growth ability of the viruses derived from the reporter constructs, the potential negative effect of the mutant vif sequence was readily and sensitively monitored. Ten vif mutant sequences tested were found not to exert the trans-dominant negative effect on the replication of HIV-1.
Fujita
Mikako
M
Department of Virology, The University of Tokushima Graduate School of Medicine, Tokushima-shi, Tokushima 770-8503, Japan. mfujita@basic.med.tokushima-u.ac.jp
Matsumoto
Sora
S
Sakurai
Akiko
A
Doi
Naoya
N
Miyaura
Maki
M
Yoshida
Akiko
A
Adachi
Akio
A
D001681:Biological Assay; D015153:Blotting, Western; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000096:biosynthesis; D016346:Gene Products, vif / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017930:Genes, Reporter; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D009154:Mutation; D014779:Virus Replication; D054320:vif Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D016346:Gene Products, vif; D054320:vif Gene Products, Human Immunodeficiency Virus