title: Time between onset of apoptosis and release of nucleosomes from apoptotic cells: putative implications for systemic lupus erythematosus.
abstract: OBJECTIVE
  To investigate the kinetics of nucleosome leakage from apoptotic cells in an in vitro system and extrapolate the results to autoimmune disease, in particular systemic lupus erythematosus.
  
  
  METHODS
  A sensitive nucleosome enzyme linked immunosorbent assay (ELISA) was developed, using a monoclonal antibody (mAb) against histone 3 and an mAb against nucleosomes. Nucleosome release during apoptotic cell death was studied in Jurkat cells. AnnexinV binding (early apoptosis) and propidium iodide positivity (late apoptosis) of the cells were compared with nucleosome release at different times after apoptosis induction.
  
  
  RESULTS
  Nucleosomes appeared in culture supernatant of Jurkat cells 24 to 48 hours after apoptosis induction, when the cells had been late apoptotic for more than 12 hours.
  
  
  CONCLUSIONS
  Nucleosomes are released from late apoptotic Jurkat cells, with a 12 hour delay from the appearance of AnnexinV binding cells. This result suggests that in vivo scavenger mechanisms have 12 hours to remove apoptotic material from the circulation.
authors.affiliation: Department of Immunopathology, Sanquin Research at CLB University of Amsterdam, 1006 AD Amsterdam, The Netherlands.
authors.forename: A E M
authors.initials: AE
authors.lastname: van Nieuwenhuijze
authors.forename: T
authors.initials: T
authors.lastname: van Lopik
authors.forename: R J T
authors.initials: RJ
authors.lastname: Smeenk
authors.forename: L A
authors.initials: LA
authors.lastname: Aarden
mesh_terms: D000974:Antibodies, Antinuclear / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017209:Apoptosis / Q000276:immunology / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006657:Histones / Q000276:immunology; D006801:Humans; D019169:Jurkat Cells; D008180:Lupus Erythematosus, Systemic / Q000276:immunology* / Q000503:physiopathology; D009707:Nucleosomes / Q000276:immunology*; D013997:Time Factors; D019014:fas Receptor / Q000276:immunology
chemical_list: D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D006657:Histones; D009707:Nucleosomes; D019014:fas Receptor