Fluorescent bioprobes for visualization of puromycin-sensitive aminopeptidase in living cells.
Non-peptide, small-molecular, non-competitive inhibitors of puromycin-sensitive aminopeptidase (PSA), that is, 3-(2,6-diethylphenyl)-2,4(1H,3H)-quinazolinedione (PAQ-22, 3) and its 1N-methyl analogue (MPAQ-22 4), were structurally modified to afford fluorescent bioprobes, ANTAQ (5) and DAMPAQ-22 (6). The cellular localization of PSA could be visualized by the use of these fluorescent bioprobes.
Kakuta
Hiroki
H
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Japan.
Koiso
Yukiko
Y
Nagasawa
Kazuo
K
Hashimoto
Yuichi
Y
D000626:Aminopeptidases / Q000032:analysis* / Q000037:antagonists & inhibitors; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D020128:Inhibitory Concentration 50; D007700:Kinetics; D008856:Microscopy, Fluorescence; D011691:Puromycin / Q000494:pharmacology; D011799:Quinazolines / Q000138:chemical synthesis / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D005456:Fluorescent Dyes; D011799:Quinazolines; D011691:Puromycin; D000626:Aminopeptidases