title: [Ex vivo expansion of CD34(+) CD59(+) cells from bone marrow of paroxysmal nocturnal hemoglobinuria patients].
abstract: OBJECTIVE
  To study the separation, purification and ex vivo expansion of CD(34)(+) CD(59)(+) cells from patients with paroxysmal nocturnal hemoglobinuria (PNH), and explore the new treatment for the PNH patients.
  
  
  METHODS
  CD(34)(+) CD(59)(+) cells were selected from the bone marrow mononuclear cells of PNH patients by means of immunomagnetic microbead-flow cytometry two step sorting method, followed by ex vivo expansion of the cells with combination of hematopoietic factors for two weeks.
  
  
  RESULTS
  The best combination for the ex vivo expansion was SCF + IL-3 + IL-6 + FL + Tpo + Epo, and the seventh day was the most suitable time for the best harvest when the CD(34)(+) CD(59)(+) cells were 22.42 +/- 3.73 fold expanded. After ex vivo expansion, the cells remained CD(59) positive and potent capacity of colony formation, but their potentialities to multilineage differentiation were decreased.
  
  
  CONCLUSIONS
  The present study shows that ex vivo expansion of CD(34)(+) CD(59)(+) cells from PNH patients might promise the possibility of performing ABMT or APBSCT clinincally for the patients.
authors.affiliation: Department of Hematology, Pekin Union Medical College Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Xiao
authors.forename: Yongji
authors.initials: Y
authors.lastname: Wu
authors.forename: Zhinan
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhaojiang
authors.initials: Z
authors.lastname: Lu
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis*; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology*; D018957:CD59 Antigens / Q000032:analysis*; D002454:Cell Differentiation / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D019070:Cell Lineage; D005434:Flow Cytometry; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood* / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D013997:Time Factors
chemical_list: D018952:Antigens, CD34; D018957:CD59 Antigens