title: Brain metabolite composition during early human brain development as measured by quantitative in vivo 1H magnetic resonance spectroscopy.
abstract: Biochemical maturation of the brain can be studied noninvasively by (1)H magnetic resonance spectroscopy (MRS) in human infants. Detailed time courses of cerebral tissue contents are known for the most abundant metabolites only, and whether or not premature birth affects biochemical maturation of the brain is disputed. Hence, the last trimester of gestation was observed in infants born prematurely, and their cerebral metabolite contents at birth and at expected term were compared with those of fullterm infants. Successful quantitative short-TE (1)H MRS was performed in three cerebral locations in 21 infants in 28 sessions (gestational age 32-43 weeks). The spectra were analyzed with linear combination model fitting, considerably extending the range of observable metabolites to include acetate, alanine, aspartate, cholines, creatines, gamma-aminobutyrate, glucose, glutamine, glutamate, glutathione, glycine, lactate, myo-inositol, macromolecular contributions, N-acetylaspartate, N-acetylaspartylglutamate, o-phosphoethanolamine, scyllo-inositol, taurine, and threonine. Significant effects of age and location were found for many metabolites, including the previously observed neuronal maturation reflected by an increase in N-acetylaspartate. Absolute brain metabolite content in premature infants at term was not considerably different from that in fullterm infants, indicating that prematurity did not affect biochemical brain maturation substantially in the studied population, which did not include infants of extremely low birthweight.
authors.affiliation: MR Spectroscopy and Methodology Unit, Department of Clinical Research, University Berne, Berne, Switzerland. roland.kreis@insel.ch
authors.forename: R
authors.initials: R
authors.lastname: Kreis
authors.forename: L
authors.initials: L
authors.lastname: Hofmann
authors.forename: B
authors.initials: B
authors.lastname: Kuhlmann
authors.forename: C
authors.initials: C
authors.lastname: Boesch
authors.forename: E
authors.initials: E
authors.lastname: Bossi
authors.forename: P S
authors.initials: PS
authors.lastname: Hüppi
mesh_terms: D001224:Aspartic Acid / Q000378:metabolism; D001921:Brain / Q000196:embryology* / Q000378:metabolism*; D001923:Brain Chemistry; D002794:Choline / Q000378:metabolism; D005314:Embryonic and Fetal Development; D005260:Female; D005865:Gestational Age; D018698:Glutamic Acid / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007234:Infant, Premature / Q000378:metabolism*; D007294:Inositol / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D012016:Reference Values; D012680:Sensitivity and Specificity
chemical_list: D001224:Aspartic Acid; D018698:Glutamic Acid; D007294:Inositol; D005978:Glutathione; D002794:Choline