Purification of long-chain fatty acid ester of epigallocatechin-3-O-gallate by high-speed counter-current chromatography.
Epigallocatechin-3-O-gallate (EGCG) was modified by catalytic esterification with hexadecanoyl chloride. A long-chain fatty acid ester derivative was obtained from the purification of this reaction product by high-speedcounter-current chromatography using a solvent system composed of n-hexane-ethyl acetate-methanol-water (1:1:1:1, v/v). The structure of the derivative, epigallocatechin-3-O-gallate-4'-hexadecanate, was elucidated by IR, MS and 1H NMR.
Chen
Ping
P
Department of Chemistry, Zhejiang University, Hangzhou 310027, China.
Wang
Huigang
H
Du
Qizhen
Q
Ito
Yoichiro
Y
D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods*; D004952:Esters / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods
D004952:Esters; D005227:Fatty Acids; D002392:Catechin; C045651:epigallocatechin gallate