Regeneration of serous membrane on gelatin-processed polyglycolic acid (PGA)-human collagen membrane and its efficacy on the prevention of adhesion.
We prepared a complex membrane consisting of human amnion-derived collagen membrane and polyglycolic acid (PGA), and then gelatin was crosslinked using heat on one side of the complex membrane to prepare a membrane that can prevent adhesion (gelatin-processed PGA-human collagen membrane). Applying this membrane to a rabbit cecum-abdominal-wall-adhesion model, the prevention of adhesion and tissue regeneration were investigated. The animals were sacrificed 2 and 12 weeks after surgery and then examined. The adhesion scores in the short-term observation group (2 weeks after surgery) and long-term observation group (12 weeks after surgery) were 1.0 +/- 2.4 and 0.8 +/- 2.0, respectively, showing a significant prevention of adhesion compared to the control value of 6.3 +/- 2.5 (p < 0.01). Histologically, gelatin was not absorbed, and outgrowth of connective tissue accompanied by capillary blood vessels was observed between the sample and the cecum in the short-term observation group. In the long-term observation group, the sample was completely absorbed, and serous membrane was regenerated on the surface of connective tissue. Based on these findings, it is possible to use gelatin-processed PGA-human collagen membrane as a filling material with both an adhesion-preventing effect and tissue-regenerating function.
Lee
Yong Ho
YH
Department of Bioartificial Organs, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8397, Japan.
Nakamura
Tatsuo
T
Shimizu
Yasuhiko
Y
Yamamoto
Yasumich
Y
Kiyotani
Tetsuya
T
Tsuda
Touru
T
Teramachi
Masayoshi
M
Takimoto
Yukinobu
Y
D000818:Animals; D003094:Collagen / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D011100:Polyglycolic Acid / Q000737:chemistry*; D011817:Rabbits; D000267:Tissue Adhesions
D011100:Polyglycolic Acid; D005780:Gelatin; D003094:Collagen