title: Novel noninvasive sensitive determination of tear volume changes in normal cats.
abstract: We describe a novel, high-resolution and noninvasive method for measuring tear volume changes in cats. The method entails photographing at the lid margin the tear meniscus area defined by instillation of 0.1% fluorescein solution into the cul-de-sac. The inferior tear meniscus area was obtained from the digitized images with computer-assisted software. The tear meniscus area increased in proportion to the saline volume applied into the conjunctival sac, which validates the technique. Furthermore, this technique detected with high sensitivity previously described increases in tear fluid secretion induced by the P2Y(2) agonist. We demonstrate in cats that changes in conjunctival sac tear volume can be evaluated by measurement of its inferior tear meniscus area.
authors.affiliation: Ophthalmic Research Division, Santen Pharmaceutical Co., Ltd., Ikoma-shi, Nara, Japan. tadahiro.murakami@santen.co.jp
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Murakami
authors.forename: Hiromi
authors.initials: H
authors.lastname: Fujita
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Fujihara
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Nakamura
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Nakata
mesh_terms: D000818:Animals; D002415:Cats; D003228:Conjunctiva / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D003941:Diagnostic Techniques, Ophthalmological; D019793:Fluorescein; D015590:Fluorophotometry / Q000379:methods; D008297:Male; D008592:Menisci, Tibial / Q000187:drug effects / Q000378:metabolism; D009883:Ophthalmic Solutions / Q000494:pharmacology; D011122:Polyphosphates; D012680:Sensitivity and Specificity; D013666:Tears / Q000378:metabolism*; D013997:Time Factors; D014500:Uracil Nucleotides
chemical_list: D009883:Ophthalmic Solutions; D011122:Polyphosphates; D014500:Uracil Nucleotides; C403315:diquafosol; D019793:Fluorescein