Methods to increase enantioselectivity of lipases and esterases.
Lipases and esterases are frequently used in the synthesis of optically pure compounds; however, natural enzymes do not always show sufficiently high enantioselectivity. Variation of the structure of the substrates, modification of the reaction system or protein engineering (e.g. the expression of pure enzymes, rational design or directed evolution) are strategies that can be employed to improve the distinction between two enantiomers or enantiotopic groups.
Bornscheuer
Uwe T
UT
Institute of Chemistry & Biochemistry, Department of Technical Chemistry & Biotechnology, Greifswald University, Soldmannstrasse 16, D-17487, Greifswald, Germany. uwe.bornscheuer@uni-greifswald.de
D004789:Enzyme Activation; D004950:Esterases / Q000096:biosynthesis* / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D019143:Evolution, Molecular; D015966:Gene Expression Regulation, Fungal; D007527:Isoenzymes / Q000096:biosynthesis / Q000737:chemistry / Q000145:classification / Q000235:genetics; D008049:Lipase / Q000096:biosynthesis* / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D008968:Molecular Conformation; D015202:Protein Engineering / Q000379:methods*; D011786:Quality Control; D012997:Solvents / Q000737:chemistry; D013379:Substrate Specificity
D007527:Isoenzymes; D012997:Solvents; D004950:Esterases; D008049:Lipase