[Optimizing histological image data for 3-D reconstruction using an image equalizer].
Bone cells form a wired network within the extracellular bone matrix. To analyse this complex 3D structure, we employed a confocal fluorescence imaging procedure to visualize live bone cells within their native surrounding. By means of newly developed image processing software, the "Image-Equalizer", we aimed to enhanced the contrast and eliminize artefacts in such a way that cell bodies as well as fine interconnecting processes were visible.
Roth
A
A
Bereich Med. Informatik, Fachhochschule Dortmund. alexander.roth@fh-dortmund.de
Melzer
K
K
Annacker
K
K
Lipinski
H G
HG
Wiemann
M
M
Bingmann
D
D
D000465:Algorithms; D000818:Animals; D016477:Artifacts; D001842:Bone and Bones / Q000166:cytology*; D002478:Cells, Cultured; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D010781:Photography / Q000295:instrumentation*; D051381:Rats