title: Voltage-gated mobility of the Ca2+ channel cytoplasmic tails and its regulatory role.
abstract: Transient increase in intracellular free Ca(2+) concentration generated by the voltage-gated Ca(v)1.2 channels acts as an important intracellular signal. By using fluorescence resonance energy transfer combined with patch clamp in living cells, we present evidence for voltage-gated mobility of the cytoplasmic tails of the Ca(v)1.2 channel and for its regulatory role in intracellular signaling. Anchoring of the C-terminal tail to the plasma membrane caused an inhibition of its state-dependent mobility, channel inactivation, and CREB-dependent transcription. Release of the tail restored these functions suggesting a direct role for voltage-gated mobility of the C-terminal tail in Ca(2+) signaling.
authors.affiliation: National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA.
authors.forename: Evgeny
authors.initials: E
authors.lastname: Kobrinsky
authors.forename: Elena
authors.initials: E
authors.lastname: Schwartz
authors.forename: Darrell R
authors.initials: DR
authors.lastname: Abernethy
authors.forename: Nikolai M
authors.initials: NM
authors.lastname: Soldatov
mesh_terms: D000818:Animals; D019556:COS Cells; D020746:Calcium Channels, L-Type / Q000032:analysis* / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D015640:Ion Channel Gating; D017434:Protein Structure, Tertiary; D015398:Signal Transduction
chemical_list: D020746:Calcium Channels, L-Type; C419385:L-type calcium channel alpha(1C)