Mutation of TRS130, which encodes a component of the TRAPP II complex, activates transcription of OCH1 in Saccharomyces cerevisiae.
The Saccharomyces cerevisiae Och1p is required for the initiation of the mannose outer chain elongation of cell wall mannoproteins. A trs130(ts1) mutant that showed an elevated expression of the OCH1-reporters was isolated in our laboratory. In this study, we attempted to gain an understanding of the relationship between the defect in the Trs130p function and the elevated expression of OCH1. The trs130(ts1) mutant showed calcofluor white-resistant and hygromycin B-sensitive phenotypes, indicating that the mutant is defective in cell wall integrity. YPT31 and YPT32, the multi-copy suppressors of the trs130(ts1) mutant, differed in the extent of suppression of the mutant phenotypes and in their genetic interaction with TRS130. Our results suggest that the blocking of vesicle transport by a loss of the Trs130p function causes some defect in the cell wall mannoproteins, which leads to the elevated expression of OCH1 through the Skn7p function, to compensate for the defect.
Yamamoto
Katsuyoshi
K
Institute of Biological Science, University of Tsukuba, Tennoudai 1-1, Tsukuba, Ibaraki 305-8577, Japan.
Jigami
Yoshifumi
Y
D001483:Base Sequence; D001557:Benzenesulfonates / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002473:Cell Wall / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D025141:Drug Resistance, Fungal / Q000235:genetics; D018628:Gene Dosage; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D006921:Hygromycin B / Q000494:pharmacology; D046911:Macromolecular Substances; D008364:Mannosyltransferases / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D013489:Suppression, Genetic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology*; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism
D001557:Benzenesulfonates; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C075636:OCH1 protein, S cerevisiae; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; C083962:SKN7 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C475918:TRS130 protein, S cerevisiae; D014157:Transcription Factors; D033921:Vesicular Transport Proteins; C406327:transport protein particle, TRAPP; D006921:Hygromycin B; C007061:C.I. Fluorescent Brightening Agent 28; D008364:Mannosyltransferases; C087905:YPT31 protein, S cerevisiae; C121633:YPT32 protein, S cerevisiae; D020691:rab GTP-Binding Proteins