Ability of structurally diverse natural products and synthetic chemicals to induce gene expression mediated by estrogen receptors from various species.
The ability of 14 structurally diverse estrogenic compounds to induce reporter gene expression mediated by estrogen receptors (ERs) from different species was examined. MCF-7 cells were transiently transfected with a Gal4-regulated luciferase reporter gene (17m5-G-Luc) and Gal4-ER chimeric receptors containing the D, E and F domains of the human alpha (Gal4-hERalphadef), mouse alpha (Gal4-mERalphadef), mouse beta (Gal4-mERbetadef), chicken (Gal4-cERalphadef), green anole (Gal4-aERalphadef), Xenopus (Gal4-xERdef) or rainbow trout alpha ERs (Gal4-rtERalphadef). The efficacy of 17beta-estradiol (E2) in inducing reporter gene expression was similar among the different constructs overall, with EC(50) values ranging from 0.05 to 0.7nM. However, Gal4-rtERalphadef had an EC(50) value at 37 degrees C of 28nM, though at 20 degrees C an EC(50) value of 1nM was observed. Despite a similar response to E2 treatment among the ERs, many differences were observed in the magnitude of the response to other structurally diverse chemicals. For example, coumestrol induced Gal4-mERbetadef- and Gal4-aERdef-mediated reporter gene expression 164- and 8-fold greater, respectively, than mediated with the other Gal4-ERs. As well, in contrast to results with other Gal4-ERs, alpha-zearalenol consistently induced Gal4-rtERalphadef-mediated reporter gene activity at lower concentrations than did E2. Overall, the results demonstrate that selected estrogenic compounds exhibit a differential ability to induce reporter gene activity mediated by ERs from different vertebrate species. These data also highlight the importance of incubation temperature when examining rtERalpha-mediated activity.
Matthews
J B
JB
Department of Biochemistry and Molecular Biology, Institute for Environmental Toxicology and National Food Safety and Toxicology Center, Michigan State University, East Lansing, MI 48824, USA.
Fertuck
K C
KC
Celius
T
T
Huang
Y-W
YW
Fong
C J
CJ
Zacharewski
T R
TR
D000818:Animals; D004268:DNA-Binding Proteins; D004958:Estradiol / Q000737:chemistry / Q000378:metabolism*; D004965:Estrogen Antagonists / Q000378:metabolism*; D020847:Estrogen Receptor Modulators / Q000378:metabolism*; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D015394:Molecular Structure; D051958:Nuclear Receptor Coactivator 2; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013223:Statistics as Topic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D004965:Estrogen Antagonists; D020847:Estrogen Receptor Modulators; D004968:Estrogens, Non-Steroidal; C052130:GAL4 protein, S cerevisiae; D051958:Nuclear Receptor Coactivator 2; D011960:Receptors, Estrogen; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D004958:Estradiol