title: Gene expression profiling using advanced mass spectrometric approaches.
abstract: In the era of systems biology, computational and high-throughput experimental biological approaches are increasingly being combined to provide global snapshots of entire genomes and proteomes under tissue- and disease-specific conditions. The aim is to identify proteins changing in concentration and/or post-translational state and/or location, and develop a better molecular level understanding of the operation of biological systems. Here we describe an approach for comparative proteomics that builds upon the combination of high-performance nano-scale separations with the high-mass measurement accuracy, mass-resolving power and sensitivity of Fourier transform ion cyclotron resonance mass spectrometry to provide broad dynamic range, comprehensive and quantitative proteome measurements.
authors.affiliation: Environmental Molecular Sciences Laboratory, MSIN: K8-98, Pacific Northwest National Laboratory, PO Box 999, Richland, Washington 99352, USA. ljiljana.pasatolic@pnl.gov
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Pasa-Tolić
authors.forename: Mary S
authors.initials: MS
authors.lastname: Lipton
authors.forename: Christophe D
authors.initials: CD
authors.lastname: Masselon
authors.forename: Gordon A
authors.initials: GA
authors.lastname: Anderson
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Shen
authors.forename: Nikola
authors.initials: N
authors.lastname: Tolić
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics; D034301:Deinococcus / Q000737:chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D036103:Nanotechnology; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D029742:Caenorhabditis elegans Proteins; D005656:Fungal Proteins