Electron capture dissociation of weakly bound polypeptide polycationic complexes.
We have previously reported that, in electron capture dissociation (ECD), rupture of strong intramolecular bonds in weakly bound supramolecular aggregates can proceed without dissociation of weak intermolecular bonds. This is now illustrated on a series of non-specific peptide-peptide dimers as well as specific complexes of modified glycopeptide antibiotics with their target peptide. The weak nature of bonding is substantiated by blackbody infrared dissociation, low-energy collisional excitation and force-field simulations. The results are consistent with a non-ergodic ECD cleavage mechanism.
Haselmann
Kim F
KF
Department of Chemistry, University of Southern Denmark, Odense, Denmark. kfh@chem.sdu.dk
Jørgensen
Thomas J D
TJ
Budnik
Bogdan A
BA
Jensen
Frank
F
Zubarev
Roman A
RA
D000900:Anti-Bacterial Agents / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D019281:Dimerization; D004583:Electrons; D006020:Glycopeptides; D006860:Hydrogen Bonding; D013058:Mass Spectrometry; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*; D014640:Vancomycin / Q000737:chemistry
D000900:Anti-Bacterial Agents; D002412:Cations; D006020:Glycopeptides; D010455:Peptides; C053759:eremomycin; D014640:Vancomycin