title: Foetal protection against bovine virus diarrhoea virus after two-step vaccination.
abstract: In order to assess the efficacy of a two-step vaccination protocol with respect to foetal protection against transplacental infections with bovine virus diarrhoea virus (BVDV) with special attention to BVDV-2 seronegative heifers were vaccinated with an inactivated BVDV-1 vaccine and boostered with a modified live BVDV-1 vaccine after 4 weeks. A second group was left unvaccinated as control. Between days 30 and 120 of pregnancy the heifers of both groups were intranasally challenged with a mixture of BVDV-1 and -2. All heifers of the vaccinated group gave birth to nine clinically healthy, seronegative (precolostral) and BVDV-free calves. In contrast in the control group four BVDV viraemic underdeveloped calves were born. Additionally, one calf was stillborn and another viraemic calf was not viable and died 2 days after birth. All six calves of the control group were viraemic with BVDV-2. This study demonstrated for the first time that two-step vaccination of breeding cattle with a modified live BVDV vaccine 4 weeks after application of an inactivated BVDV vaccine was capable of providing a foetal protection against transplacental infection with BVDV-2.
authors.affiliation: Institute of Virology, School of Veterinary Medicine, Buenteweg 17, D-30559 Hannover, Germany.
authors.forename: H-R
authors.initials: HR
authors.lastname: Frey
authors.forename: K
authors.initials: K
authors.lastname: Eicken
authors.forename: B
authors.initials: B
authors.lastname: Grummer
authors.forename: S
authors.initials: S
authors.lastname: Kenklies
authors.forename: T C
authors.initials: TC
authors.lastname: Oguzoglu
authors.forename: V
authors.initials: V
authors.lastname: Moennig
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000235:genetics / Q000302:isolation & purification; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000517:prevention & control* / Q000635:transmission / Q000821:virology; D002417:Cattle; D030241:Diarrhea Virus 1, Bovine Viral / Q000276:immunology* / Q000472:pathogenicity; D030242:Diarrhea Virus 2, Bovine Viral / Q000276:immunology* / Q000472:pathogenicity; D004334:Drug Administration Schedule; D005260:Female; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control / Q000662:veterinary; D007279:Injections, Subcutaneous / Q000662:veterinary; D009500:Neutralization Tests / Q000662:veterinary; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000517:prevention & control / Q000662:veterinary; D014611:Vaccination; D015164:Vaccines, Inactivated; D014765:Viral Vaccines
chemical_list: D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines