PAGE4 is a cytoplasmic protein that is expressed in normal prostate and in prostate cancers.
PAGE4 is an X chromosome-linked cancer-testis antigen that was identified by expressed sequence tags database mining and a functional genomic approach. PAGE4 is preferentially expressed in normal male and female reproductive tissues and also in a variety of cancers including prostate. In the present study, we have used in situ hybridization to show that PAGE4 mRNA is expressed only in the epithelial cells of normal and prostate-cancer specimens. Analysis of the protein product encoded by the PAGE4 mRNA reveals that it encodes a Mr 16,000 protein and is detected in tissue extracts from both normal prostate and prostate cancer. Cell fractionation analysis of PAGE4 protein indicates that PAGE4 is localized in the cytoplasm of the cell. Furthermore, cDNA microarray analysis indicates that the expression of lipoprotein lipase, a gene frequently deleted in prostate cancer, is down-regulated in a cell line that expresses PAGE4.
Iavarone
Carlo
C
Laboratory of Molecular Biology, Clinical Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA.
Wolfgang
Curt
C
Kumar
Vasantha
V
Duray
Paul
P
Willingham
Mark
M
Pastan
Ira
I
Bera
Tapan K
TK
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm / Q000096:biosynthesis; D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism*; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D008040:Genetic Linkage; D006801:Humans; D017403:In Situ Hybridization; D008071:Lipoprotein Lipase / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014407:Tumor Cells, Cultured; D014960:X Chromosome
D000951:Antigens, Neoplasm; D018076:DNA, Complementary; C474505:PAGE4 protein, human; D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D008071:Lipoprotein Lipase