title: Production of macrophage inflammatory protein (MIP)-1alpha and MIP-1beta by human polymorphonuclear neutrophils stimulated with Porphyromonas endodontalis lipopolysaccharide.
abstract: This study was undertaken to investigate the capacity of polymorphonuclear neutrophils (PMNs) to secrete Macrophage Inflammatory Protein (MIP)-1alpha and MIP-1beta after stimulation with Porphyromonas endodontalis lipopolysaccharide (LPS). Escherichia coli LPS was used as a positive control. Venous blood was collected and PMNs were isolated from healthy volunteers. Cells were cultured with various concentrations of LPS for different periods of time. Cell supernatants were assayed by enzyme-linked immunosorbent assay. The levels of chemokine secretion in PMNs stimulated with each LPS were found to be significantly higher than in the unstimulated control cells (p < 0.05), and this expression occurred in a time- and dose-dependent manner. E. coli LPS induced higher levels of cytokines than P. endodontalis LPS. These findings demonstrated that P. endodontalis LPS is capable of stimulating PMNs to produce chemotactic cytokines and suggested that PMNs stimulated with P. endodontalis LPS may play a crucial role in the inflammatory and immunopathological reactions of pulpal and periapical diseases.
authors.affiliation: Department of Conservative Dentistry, College of Dentistry, Seoul National University, Korea.
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Ko
authors.forename: Sung Sam
authors.initials: SS
authors.lastname: Lim
mesh_terms: D016475:3T3 Cells; D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D003788:Dental Pulp Diseases / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology; D005347:Fibroblasts / Q000276:immunology; D006801:Humans; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000276:immunology*; D019402:Macrophage Inflammatory Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D051379:Mice; D020556:Neutrophil Infiltration / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D010483:Periapical Diseases / Q000276:immunology; D010513:Periodontal Ligament / Q000166:cytology / Q000276:immunology; D017861:Porphyromonas / Q000276:immunology*; D018709:Statistics, Nonparametric; D013997:Time Factors
chemical_list: D054405:Chemokine CCL3; D054407:Chemokine CCL4; D008070:Lipopolysaccharides; D019402:Macrophage Inflammatory Proteins