Epidermal reconstructs: a new tool to study topical and systemic photoprotective molecules.
In this study, we compared the effects of sunscreens and antioxidants on reconstructed epidermis made with or without melanocytes 24 h after UVB, UVA or UVA+B irradiation. For this purpose, we studied sunburn cells and cyclobutane pyrimidine dimer formation, protein and lipid oxidation, catalase and superoxide dismutase activities and vitamin E levels. Topical sunscreens protected against direct cell death and thymine dimer formation whereas their protective effect against protein and lipid oxidation and antioxidant depletion was less marked partly due to the difficulty of spreading the cream. Antioxidant molecules protected against direct cell death and protein oxidation but not against thymine dimer formation. Since topical sunscreens and systemic antioxidant protected the skin differently, we speculate that their association might protect more efficiently against UV-induced damage. This model is relevant to study systemic molecules but is less practical, due to the technical limitations of studying topical molecules.
Cario-André
Muriel
M
Unité de Dermatologie, Laboratoire de Pathologie Moléculaire et Thérapie Génique, INSERM 0217, Université Victor Ségalen, 146 rue Léo Saignat, Batiment TP ouest, 4ème étage, 33076 Bordeaux Cedex, France. muriel.cario-andre@dermatol.u-bordeaux2.fr
Briganti
Stefania
S
Picardo
Mauro
M
Nikaido
Osamu
O
Gall
Yvon
Y
Ginestar
José
J
Taïeb
Alain
A
D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D002944:Circumcision, Male; D004817:Epidermis / Q000187:drug effects / Q000528:radiation effects*; D006801:Humans; D007231:Infant, Newborn; D015227:Lipid Peroxidation; D008297:Male; D008544:Melanocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D011740:Pyrimidine Dimers / Q000032:analysis; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D012016:Reference Values; D013471:Sunburn / Q000473:pathology; D013473:Sunscreening Agents / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays; D014810:Vitamin E / Q000378:metabolism
D000975:Antioxidants; D011740:Pyrimidine Dimers; D011837:Radiation-Protective Agents; D013473:Sunscreening Agents; D014810:Vitamin E; D002374:Catalase; D013482:Superoxide Dismutase