title: Detection and characterization of membrane-bound selenoproteins in the kidney of the rat.
abstract: The essential trace element selenium is of fundamental importance to human biology. Information on the membrane-bound selenoproteins was obtained by labeling of rats in vivo with (75)Se-selenite, subcellular fractionation of the tissue homogenates, separation of the proteins by SDS-PAGE, two-dimensional electrophoresis, and selenium detection by autoradiography. In this way, in addition to already known membrane-bound selenoproteins three novel selenoproteins in the molecular mass range of 38-40, 18, and 12-13 kDa were detected in the microsomal membranes.
authors.affiliation: Department of Molecular Trace Element Research in the Life Sciences, Hahn-Meitner-Institut, D-14109 Berlin, Germany. kyriakopoulos@hmi.de
authors.forename: A
authors.initials: A
authors.lastname: Kyriakopoulos
authors.forename: H
authors.initials: H
authors.lastname: Bertelsmann
authors.forename: A
authors.initials: A
authors.lastname: Graebert
authors.forename: B
authors.initials: B
authors.lastname: Hoppe
authors.forename: M
authors.initials: M
authors.lastname: Kuhbacher
authors.forename: D
authors.initials: D
authors.lastname: Behne
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D007668:Kidney / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000172:deficiency*; D012644:Selenium Radioisotopes; D051140:Selenoproteins
chemical_list: D000596:Amino Acids; D011506:Proteins; D012644:Selenium Radioisotopes; D051140:Selenoproteins; D012643:Selenium