[Affection of neoadjuvant chemotherapy on cell apoptosis and proliferation in breast cancer].
OBJECTIVE
Although the researches had verified various kinds of chemotherapic agents can cause the apoptosis of breast cancer cells, but the reports with in vivo data are still scarce. This paper is to investigate whether neoadjuvant chemotherapy could induce tumor cell apoptosis in breast cancer patients, and its effect on cell proliferation.


METHODS
Apoptosis index (AI) of tumor cells is assayed by TdT-mediated dUTP nick end labeling(TUNEL) and proliferating cell nuclear antigen (PCNA) is examined by immunohistochemical labelled streptavidin biotin (ISAB) in 100 beast cancer samples.


RESULTS
Tumor cell AI in neoadjuvant group (mean = 7.47%) was significantly higher than that in control group (mean = 4.83%) (P < 0.01). PCNA positive expression rate in neoadjuvant group (mean = 33.71%) was significantly lower than that in control group (mean = 51.52%) (P < 0.01). There was significant negative association between AI and PCNA in both neoadjuvant chemotherapy group and control group (P < 0.05).


CONCLUSIONS
Neoadjuvant chemotherapy could induce tumor cell apoptosis and inhibit tumor cell proliferation in human breast cancer.
Zhan
You-qing
YQ
Department of Abdomen, Cancer Center, Sun Yat-sen University, Guangzhou 510060, P. R. China. yq_zhan@21cn.com
Xu
Li
L
Sun
Xiao-wei
XW
Zhong
Jian
J
Li
Wei
W
D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D017024:Chemotherapy, Adjuvant; D005260:Female; D006801:Humans; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis
D018809:Proliferating Cell Nuclear Antigen