[Effects of hexamethylene bisacetamide on related genes expression of apoptosis and cell proliferating in human mucoepidermoid carcinoma cell line MEC-1].
OBJECTIVE
To investigate the effects of hexamethylene bisacetamide (HMBA) on related genes expression of apoptosis and cell proliferating in human mucoepidermoid carcinoma cell line MEC-1.


METHODS
MEC-1 was induced 72 hours by 0.002 mol/L HMBA in vitro. The morphologic observation and the expression of PCNA, bcl-2, bax protein and bcl-2 mRNA were determined by microscope, immunohistochemistry, in situ hybridization and image analysis.


RESULTS
The expression of bax, bcl-2 protein and bcl-2 mRNA were higher than control group (P < 0.01). The related genes of cell proliferating PCNA were decreased in control group.


CONCLUSIONS
HMBA could inhibit the MEC-1 cell proliferation by down-regulation of PCNA expression, it could also induce apoptosis effectively that might be through the way of up-regulation of bax and bcl-2 gene expression.
Wang
Lijing
L
Department of Stomatology, Lanzhou Medical College, Lanzhou 730000, China.
Wang
Jing
J
D000081:Acetamides / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000235:genetics*; D018277:Carcinoma, Mucoepidermoid / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D019254:Genes, bcl-2; D006801:Humans; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
D000081:Acetamides; D000970:Antineoplastic Agents; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; C014026:hexamethylene bisacetamide