A "step down" PCR-based technique for walking into and the subsequent direct-sequence analysis of flanking genomic DNA.
Zhang
Ziguo
Z
Department of Plant Sciences, University of Oxford, Oxford, UK.
Gurr
Sarah Jane
SJ
D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D017931:DNA Primers; D007202:Indicators and Reagents; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping / Q000379:methods; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*
D017931:DNA Primers; D007202:Indicators and Reagents; D004247:DNA