Humic acids isolated from earthworm compost enhance root elongation, lateral root emergence, and plasma membrane H+-ATPase activity in maize roots.
Earthworms (Eisenia foetida) produce humic substances that can influence plant growth by mechanisms that are not yet clear. In this work, we investigated the effects of humic acids (HAs) isolated from cattle manure earthworm compost on the earliest stages of lateral root development and on the plasma membrane H(+)-ATPase activity. These HAs enhance the root growth of maize (Zea mays) seedlings in conjunction with a marked proliferation of sites of lateral root emergence. They also stimulate the plasma membrane H(+)-ATPase activity, apparently associated with an ability to promote expression of this enzyme. In addition, structural analysis reveals the presence of exchangeable auxin groups in the macrostructure of the earthworm compost HA. These results may shed light on the hormonal activity that has been postulated for these humic substances.
Canellas
Luciano Pasqualoto
LP
Centro de Ciências Tecnologias Agropecuárias, Laboratório de Solos, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, CEP 28015-620, Campos dos Goytacazes, Rio de Janeiro, Brazil.
Olivares
Fabio Lopes
FL
Okorokova-Façanha
Anna L
AL
Façanha
Arnoldo Rocha
AR
D000818:Animals; D002247:Carbon Isotopes; D002417:Cattle; D006812:Humic Substances / Q000737:chemistry / Q000494:pharmacology*; D007210:Indoleacetic Acids / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008372:Manure / Q000032:analysis; D009835:Oligochaeta / Q000737:chemistry*; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D012987:Soil / Q000032:analysis; D003313:Zea mays / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*
D002247:Carbon Isotopes; D006812:Humic Substances; D007210:Indoleacetic Acids; D008372:Manure; D012987:Soil; C030737:indoleacetic acid; D006180:Proton-Translocating ATPases