Hematopoietic capacity of connexin43 wild-type and knock-out fetal liver cells not different on wild-type stroma.
In 1995 and 1997 we proposed that gap junctions between stromal and hematopoietic cells formed by connexin43 (Cx43) determine hematopoiesis. If this were the case, are the critical gap junctions in this regard those between hematopoietic and stromal cells, or those between stromal cells alone? To test the first possibility, we compared hematopoietic repopulating capacity between fetal liver hematopoietic cells expressing the different mouse Cx43 genotypes, wild type (WT), hemizygous, or knock-out (KO) on WT host mice stroma. We deleted host glucose phosphate isomerase 1(a) (Gpi-1(a)) stems and then raced identifiable Cx43 WT host fetal liver against congenic donor Cx43 WT, hemizygous, or KO cells in sets, comparing their capacity to form 5 end cells. Hematopoietic capacity did not differ between the Cx43 WT and KO genotypes. The role of Cx43 gap junctions in hematopoiesis remains uncertain.
Rosendaal
Martin
M
Department of Anatomy and Developmental Biology, University College, London, United Kingdom. m.rosendaal@ucl.ac.uk
Jopling
Chris
C
D000818:Animals; D020296:Animals, Congenic; D002450:Cell Communication; D003114:Colony-Forming Units Assay; D018031:Connexin 43 / Q000235:genetics / Q000502:physiology*; D017630:Connexins / Q000235:genetics / Q000502:physiology; D005472:Fluorouracil / Q000633:toxicity; D017629:Gap Junctions / Q000502:physiology*; D005819:Genetic Markers; D005838:Genotype; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics; D006411:Hematopoiesis, Extramedullary / Q000235:genetics / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000196:embryology*; D051379:Mice; D018345:Mice, Knockout; D010659:Phenylhydrazines / Q000633:toxicity; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism
D018031:Connexin 43; D017630:Connexins; D005819:Genetic Markers; D010659:Phenylhydrazines; C082920:connexin 45; C030299:phenylhydrazine; D005956:Glucose-6-Phosphate Isomerase; D005472:Fluorouracil