title: Studies of the mechanism of development of "deprivation" potentiation of population responses of neurons in field CA1 of living hippocampal slices.
abstract: Experiments on rat hippocampal slices were performed with testing of the synaptic connections of Schaffer collaterals and neurons in field CA1 to study the effects of interrupting low-frequency test stimulation (0.05 Hz) on the amplitude of population spikes. These studies demonstrated a correlation between the duration of pauses in stimulation (form 10 to 120 min) and increases in the amplitude of spikes (on average by 30-100% of baseline response magnitude). This "deprivation" potentiation was additive and could persist for long periods of time (testing was up to 1 h). Preliminary induction of long-term post-tetanic potentiation, which prevented the subsequent development of the late, but not the shortterm phase, for 1-3 h, led to suppression of the development of "deprivation" potentiation after a 60-min pause in stimulation. Similar results were obtained in experiments using 20 microM polymyxin B, which blocks protein kinase C and the PKC-dependent phase of long-term post-tetanic potentiation; this is evidence supporting the previously advanced hypothesis that the development of deprivation potentiation and the late. PKC-dependent phase of long-term post-tetanic potentiation share common mechanisms, associated with people phosphorylation.
authors.affiliation: Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow.
authors.forename: V A
authors.initials: VA
authors.lastname: Popov
authors.forename: V A
authors.initials: VA
authors.lastname: Markevich
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D006624:Hippocampus / Q000502:physiology*; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000502:physiology; D008297:Male; D009474:Neurons / Q000502:physiology*; D011112:Polymyxin B / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000502:physiology
chemical_list: D004791:Enzyme Inhibitors; D011493:Protein Kinase C; D011112:Polymyxin B