title: Humoral immune response against 38-kDa and 16-kDa mycobacterial antigens in bone and joint tuberculosis.
abstract: METHODS
  The diagnosis of bone and joint tuberculosis (BTB) is difficult, and diagnostic delays often occur. A reliable serological test detecting anti-mycobacterial antibodies would thus be of some use in this form of the disease.
  
  
  OBJECTIVE
  To evaluate the diagnostic accuracy of an assay detecting IgG against 38-kDa and 16-kDa recombinant mycobacterial antigens in BTB.
  
  
  METHODS
  In a prospective study, serum samples from 124 subjects were examined: 30 BTB cases, 40 non-specific bone and joint infection patients (NSBI), 30 lung cancer patients (LC), and 24 healthy volunteers (HC). An ELISA-based test (Pathozyme TB complex plus) was used.
  
  
  RESULTS
  The cut-off level was established at 150 U/ml according to receiver operating characteristic (ROC) curves. The quantified level of sensitivity of the test detecting BTB was 56%, at a specificity of 99%. The positive and negative predictive values were respectively 94% and 88%. Mean IgG level in the BTB group was 470 +/- 761 U/ml (mean +/- SD), and was significantly higher than the antibody level in the control groups (NSBI 58 +/- 42 U/ml, LC 43 +/- 38 U/ml, HC 40 +/- 29 U/ml).
  
  
  CONCLUSIONS
  The test presents an acceptable level of sensitivity and very good specificity in the diagnosis of BTB, and can be used in combination with other methods to increase diagnostic accuracy in this disease.
authors.affiliation: Dept of Laboratory Diagnostic and Immunology, Institute of Tuberculosis and Lung Diseases, Warsaw, Poland. u.demkow@igichp.edu.pl
authors.forename: U
authors.initials: U
authors.lastname: Demkow
authors.forename: T M
authors.initials: TM
authors.lastname: Zielonka
authors.forename: M
authors.initials: M
authors.lastname: Nowak-Misiak
authors.forename: M
authors.initials: M
authors.lastname: Filewska
authors.forename: B
authors.initials: B
authors.lastname: Bialas
authors.forename: J
authors.initials: J
authors.lastname: Strzalkowski
authors.forename: K
authors.initials: K
authors.lastname: Rapala
authors.forename: Z
authors.initials: Z
authors.lastname: Zwolska
authors.forename: E
authors.initials: E
authors.lastname: Skopinska-Rozewska
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008074:Lipoproteins / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D014394:Tuberculosis, Osteoarticular / Q000276:immunology*
chemical_list: C075966:Ag38 antigen, Mycobacterium tuberculosis; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D008074:Lipoproteins; C072681:Mycobacterium tuberculosis antigens