title: Manufacture of porous polymer nerve conduits by a novel low-pressure injection molding process.
abstract: A method to fabricate porous, biodegradable conduits using a combined injection molding, thermally induced phase transition technique was developed which produced conduits with dimensionally toleranced, longitudinally aligned channels. The geometry of the channels was designed to approximate the architecture of peripheral nerves and to support the monolayer adherence of physiologically relevant numbers of Schwann cells. The channel configuration could be varied from a single 1.35 mm diameter channel up to 100 0.08 mm diameter channels. A conduit with 100 channels has approximately 12.5 times the lumenal surface area of a single channel conduit and supports the adherence of five times the number of Schwann cells in the native peripheral nerve. In this study, poly(DL-lactide-co-glycolide) (DL-PLGA) was dissolved in acetic acid and injected into a cold mold which induced solid-liquid phase separation and, ultimately, solidification of the polymer solution. The acetic acid was removed by sublimation and the resulting foam had a macrostructure of high anisotropy. Semi-permeable skins formed on the outer and lumen diameters of the conduit as a consequence of rapid quenching. Macropores were organized into bundles of channels, up to 20 microm wide, in the DL-PLGA matrix and represented remnants of acetic acid that crystallized during solidification.
authors.affiliation: Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Wellman 625 55 Fruit Street, Boston 02114, USA. csundback@partners.org
authors.forename: Cathryn
authors.initials: C
authors.lastname: Sundback
authors.forename: Tessa
authors.initials: T
authors.lastname: Hadlock
authors.forename: Mack
authors.initials: M
authors.lastname: Cheney
authors.forename: Joseph
authors.initials: J
authors.lastname: Vacanti
mesh_terms: D000818:Animals; D001673:Biodegradation, Environmental; D001704:Biopolymers; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D009584:Nitrogen; D017933:Peripheral Nervous System / Q000502:physiology*; D011098:Polyglactin 910 / Q000737:chemistry*; D016062:Porosity; D019736:Prostheses and Implants; D012583:Schwann Cells / Q000502:physiology; D013193:Stainless Steel; D013499:Surface Properties; D013816:Thermodynamics; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001704:Biopolymers; D013193:Stainless Steel; D011098:Polyglactin 910; D009584:Nitrogen