title: Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set.
abstract: A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ~120,000 cDNA clones, which were initially re-arrayed into a set of ~11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.
authors.affiliation: Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging (NIA), National Institutes of Health, Baltimore, Maryland, 20892, USA.
authors.forename: Vincent
authors.initials: V
authors.lastname: VanBuren
authors.forename: Yulan
authors.initials: Y
authors.lastname: Piao
authors.forename: Dawood B
authors.initials: DB
authors.lastname: Dudekula
authors.forename: Yong
authors.initials: Y
authors.lastname: Qian
authors.forename: Mark G
authors.initials: MG
authors.lastname: Carter
authors.forename: Patrick R
authors.initials: PR
authors.lastname: Martin
authors.forename: Carole A
authors.initials: CA
authors.lastname: Stagg
authors.forename: Uwem C
authors.initials: UC
authors.lastname: Bassey
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Aiba
authors.forename: Toshio
authors.initials: T
authors.lastname: Hamatani
authors.forename: George J
authors.initials: GJ
authors.lastname: Kargul
authors.forename: Amber G
authors.initials: AG
authors.lastname: Luo
authors.forename: Janet
authors.initials: J
authors.lastname: Kelso
authors.forename: Winston
authors.initials: W
authors.lastname: Hide
authors.forename: Minoru S H
authors.initials: MS
authors.lastname: Ko
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000235:genetics*; D001483:Base Sequence / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D019295:Computational Biology / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics*; D030541:Databases, Genetic; D004622:Embryo, Mammalian / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D015723:Gene Library; D005796:Genes / Q000235:genetics; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018076:DNA, Complementary