title: Evidence for packaging of rep-cap sequences into adeno-associated virus (AAV) type 2 capsids in the absence of inverted terminal repeats: a model for generation of rep-positive AAV particles.
abstract: We previously reported that a 350-bp region of the adeno-associated virus (AAV) type 2 rep gene contains a cis-acting element responsible for the Rep-dependent replication of a transiently transfected rep-cap plasmid. In this study, we further report that replicated rep-cap sequences can be packaged into AAV capsids in the absence of the inverted terminal repeats.
authors.affiliation: INSERM ERM 0105, CHU Hotel-Dieu, 44035 Nantes, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Nony
authors.forename: Gilliane
authors.initials: G
authors.lastname: Chadeuf
authors.forename: Jacques
authors.initials: J
authors.lastname: Tessier
authors.forename: Philippe
authors.initials: P
authors.lastname: Moullier
authors.forename: Anna
authors.initials: A
authors.lastname: Salvetti
mesh_terms: D002213:Capsid / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000235:genetics*; D000229:Dependovirus / Q000502:physiology*; D010957:Plasmids; D020079:Terminal Repeat Sequences; D014764:Viral Proteins / Q000235:genetics*; D014771:Virion / Q000502:physiology*; D019065:Virus Assembly
chemical_list: D004268:DNA-Binding Proteins; D014764:Viral Proteins; C068734:rep proteins, Adeno-associated virus 2