title: [Investigation of progesterone receptor on human sperm plasma membrane].
abstract: OBJECTIVE
  To investigate the localization and positive percentage of progesterone receptor (PR) on the human sperm surface.
  
  
  METHODS
  After in vitro capacitation, progesterone binding sites on the sperm were quantitatively analyzed by fluorescence microscopy and flow cytometry using fluorescein isothiocyanate-labeled bovine serum albumin-progesterone complex (P-BSA-FITC).
  
  
  RESULTS
  The spermatozoa stained by P-BSA-FITC mainly showed two labeling patterns, with the green fluorescence on the whole acrosomal region or the equatorial acrosomal region only and the stainless postacrosomal and tail regions. The percentage of progesterone-binding sperm was (30.2 +/- 2.4)%.
  
  
  CONCLUSIONS
  There is selective expression of PR on the human sperm acrosome surface.
authors.affiliation: Department of Histology and Embryology, Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
authors.forename: Yong-Wei
authors.initials: YW
authors.lastname: Huo
authors.forename: Shu-Dong
authors.initials: SD
authors.lastname: Qiu
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Xu
authors.forename: Shao-Li
authors.initials: SL
authors.lastname: Cheng
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Ge
mesh_terms: D000328:Adult; D002462:Cell Membrane / Q000737:chemistry; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D011980:Receptors, Progesterone / Q000032:analysis*; D013094:Spermatozoa / Q000737:chemistry*
chemical_list: D011980:Receptors, Progesterone