title: Amino acid and peptide analysis using derivatization with p-nitrophenol-2,5-dihydroxyphenylacetate bis-tetrahydropyranyl ether and capillary electrophoresis with electrochemical detection.
abstract: The amine derivatization reagent p-nitrophenol-2,5-dihydroxyphenylacetate bis-tetrahydropyranyl ether (NDTE) was used in conjunction with capillary electrophoresis (CE) and electrochemical detection (EC) for the pre-separation derivatization of primary amine analytes present in aqueous solution. Glycine, several dipeptides and angiotensin II were used as model analytes. A miniaturized EC detection cell was designed and fabricated, which featured a fractured-joint field decoupler with a fixed end-column carbon fiber electrode. When a series of glycine and angiotensin II calibration solutions were derivatized with NDTE followed by CE-EC determination, linear calibration plots resulted with pre-derivatization concentration limits of detection of 500 nM (106 attomoles on-column) and 6 microM (1.275 femtomoles on-column), respectively.
authors.affiliation: Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66047, USA.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Rose
authors.forename: Susan M
authors.initials: SM
authors.lastname: Lunte
authors.forename: Robert G
authors.initials: RG
authors.lastname: Carlson
authors.forename: John F
authors.initials: JF
authors.lastname: Stobaugh
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D011753:Pyrones / Q000032:analysis* / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000379:methods*
chemical_list: C119853:4-nitrophenyl-2,5-dihydroxyphenylacetate-bis-tetrahydropyranyl ether; D000596:Amino Acids; D010455:Peptides; D011753:Pyrones