[In vitro expression of human factor VIII gene induced by sodium butyrate].
OBJECTIVE
To explore the effect and mechanism of sodium butyrate on expression of human clotting factor VIII in vitro.


METHODS
Mouse NIH/3T3 cell line was transfected with recombinant plasmid vector pRC/RSV-BDD-hFVIII, which enclosed B-domain deleted (760aa approximately 1 639aa) human factor VIII cDNA (BDD-hFVIII cDNA). Then cells were incubated in Dulbecco's modification of Eagle's medium (DMEM) containing sodium butyrate for 24 hours, hFVIII: C and hFVIII: Ag in the cell culture medium were measured by ELISA assay and one-stage method, respectively. In addition, the effect of sodium butyrate on transcription of cDNA encoding the whole hFVIII, heavy and light chain of hFVIII was also investigated by means of run-on assay.


RESULTS
After stimulation of sodium butyrate, the levels of hFVIII: C and hFVIII: Ag increased 70% than those of control. Run-on assay showed that sodium butyrate enhanced the transcription of cDNA which encoded heavy chain of hFVIII.


CONCLUSIONS
Sodium butyrate can improve the expression of hFVIII through enhancing the transcription of hFVIII heavy chain encoding cDNA. It demonstrated that sodium butyrate had potential utility in inducing the expression of hFVIII in vitro.
Yin
Jun
J
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Wang
Hongli
H
Wang
Xuefeng
X
Chu
Haiyan
H
Li
Dao
D
Chen
Hongbing
H
Fu
Qihua
Q
Duan
Baohua
B
Kang
Wenying
W
Ding
Qiulan
Q
Qi
Zhengwu
Z
Wang
Zhenyi
Z
D016475:3T3 Cells; D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D005169:Factor VIII / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D051379:Mice
D002087:Butyrates; D005169:Factor VIII