Effect of retinoblastoma gene on human osteosarcoma cell line OS732.
OBJECTIVE
To investigate the effect of growth suppression and gap junctional intercellular communication (GJIC) on osteosarcoma cell line OS732 transduced with the exogenous N-terminal truncated retinoblastoma (Rb) gene.


METHODS
Recombinant eukaryotic expression plasmid harbouring 1.65 kb Rb gene was constructed and transduced into OS732 cells. Rb mRNA expression was detected by RT-PCR and Northern blot. The cell count, cell cycle analysis and soft agar colony formation test were used to observe growth features. RT-PCR and Lucifer yellow dye transfer were carried out to measure GJIC.


RESULTS
Both exogenous and endogenous Rb gene could be detected in OS732 cell transduced with the N-terminal truncated Rb gene. Compared with the parental cells, change in morphology of Rb-transduced cells were found; growth rate decreased; amount of colony formation reduced; cell cycle arrested at G(0)/G(1) checkpoint; Connexin43 expression and GJIC enhanced.


CONCLUSIONS
The malignant phenotype of OS732 is inhibited and GJIC is increased by transduction of N-terminal truncated Rb gene.
Qiao
Hui
H
Department of Pathology, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China (Email: qiaohui99@163.net)
Qiu
Jushi
J
Li
Yang
Y
Zhang
Meng
M
D001859:Bone Neoplasms; D002450:Cell Communication; D002460:Cell Line; D016161:Genes, Retinoblastoma; D006801:Humans; D012516:Osteosarcoma; D014407:Tumor Cells, Cultured