Solution 1H NMR investigation of the active site molecular and electronic structures of substrate-bound, cyanide-inhibited HmuO, a bacterial heme oxygenase from Corynebacterium diphtheriae.
The molecular structure and dynamic properties of the active site environment of HmuO, a heme oxygenase (HO) from the pathogenic bacterium Corynebacterium diphtheriae, have been investigated by (1)H NMR spectroscopy using the human HO (hHO) complex as a homology model. It is demonstrated that not only the spatial contacts among residues and between residues and heme, but the magnetic axes that can be related to the direction and magnitude of the steric tilt of the FeCN unit are strongly conserved in the two HO complexes. The results indicate that very similar contributions of steric blockage of several meso positions and steric tilt of the attacking ligand are operative. A distal H-bond network that involves numerous very strong H-bonds and immobilized water molecules is identified in HmuO that is analogous to that previously identified in hHO (Li, Y., Syvitski, R. T., Auclair, K., Wilks, A., Ortiz de Montellano, P. R., and La Mar, G. N. (2002) J. Biol. Chem. 277, 33018-33031). The NMR results are completely consistent with the very recent crystal structure of the HmuO.substrate complex. The H-bond network/ordered water molecules are proposed to orient the distal water molecule near the catalytically key Asp(136) (Asp(140) in hHO) that stabilizes the hydroperoxy intermediate. The dynamic stability of this H-bond network in HmuO is significantly greater than in hHO and may account for the slower catalytic rate in bacterial HO compared with mammalian HO.
Li
Yiming
Y
Department of Chemistry, University of California, Davis, California 95616, USA.
Syvitski
Ray T
RT
Chu
Grace C
GC
Ikeda-Saito
Masao
M
Mar
Gerd N La
GN
D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry; D001426:Bacterial Proteins; D001665:Binding Sites; D002384:Catalysis; D003353:Corynebacterium diphtheriae / Q000201:enzymology* / Q000378:metabolism; D018360:Crystallography, X-Ray; D003486:Cyanides / Q000494:pharmacology*; D006418:Heme / Q000737:chemistry; D006419:Heme Oxygenase (Decyclizing) / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D006859:Hydrogen; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D011485:Protein Binding; D011522:Protons; D014867:Water / Q000737:chemistry
D001426:Bacterial Proteins; D003486:Cyanides; D008024:Ligands; D011522:Protons; D014867:Water; D001224:Aspartic Acid; D006418:Heme; D006859:Hydrogen; D006419:Heme Oxygenase (Decyclizing); C104107:HmuO protein, bacteria