Stromal/epithelial interactions of murine prostatic cell lines in vivo: a model for benign prostatic hyperplasia and the effect of doxazosin on tissue size.
BACKGROUND
One of the major constraints in elucidating the mechanisms involved in the etiology of benign prostatic hyperplasia (BPH) is the lack of suitable model systems that are readily manipulable in vitro and in vivo. To address this issue, we have used murine prostatic cell lines to establish a novel in vivo model for studying prostatic cell interactions.


METHODS
Luminal, basal, and smooth muscle (SM) cell lines were inoculated alone or in combinations under the renal capsule of intact or castrated male mice, and the growth and composition of prostatic tissue in the absence or presence of doxazosin was determined.


RESULTS
Both the luminal and basal cell lines reconstituted prostatic tissue if co-inoculated under the renal capsule with normal SM cells, whereas none of the lines formed significant tissue when inoculated alone. Luminal cells produced and secreted prostatic secretory products. The growth of prostatic tissue formed from co-inoculation of basal and SM cells was androgen responsive. In addition, a significant reduction in prostatic tissue was noted in animals treated with doxazosin.


CONCLUSIONS
We have established an in vivo model that uses prostatic epithelial and SM cell lines for investigating cellular interactions between epithelial and SM cells that regulate prostatic growth and function. This model will be useful for delineating the mechanisms by which prostatic cells interact and in determining the efficacy of new approaches aimed at interfering with prostatic stromal/epithelial interactions that result in abnormal cellular proliferation.
Takao
Tetsuya
T
Department of Cell Biology, New York University School of Medicine, New York, New York 10016, USA.
Tsujimura
Akira
A
Coetzee
Sandra
S
Salm
Sarah N
SN
Lepor
Herbert
H
Shapiro
Ellen
E
Moscatelli
David
D
Wilson
E Lynette
EL
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology*; D000818:Animals; D002450:Cell Communication; D002460:Cell Line; D004195:Disease Models, Animal; D017292:Doxazosin / Q000494:pharmacology*; D004847:Epithelial Cells / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009130:Muscle, Smooth / Q000166:cytology; D011470:Prostatic Hyperplasia / Q000188:drug therapy / Q000503:physiopathology*; D017154:Stromal Cells / Q000502:physiology
D000317:Adrenergic alpha-Antagonists; D017292:Doxazosin