title: Characterization of gamma-tocopherol methyltransferases from Capsicum annuum L and Arabidopsis thaliana.
abstract: Tocopherols are essential micronutrients in human and animal nutrition due to their function as lipophilic antioxidants. They are exclusively synthesized by photosynthetic organisms including higher plants. Despite the attributed beneficial health effects and many industrial applications, research on the tocopherol biosynthetic pathway and its regulation in plants is still limited. In the work presented here we performed a detailed biochemical characterization of a gamma-tocopherol methyltransferase (gamma-TMT) from Arabidopsis thaliana and of a gamma-TMT purified from Capsicum annuum fruits, a tissue with high accumulation of tocopherols. The biochemical characteristics of both enzyme preparations were remarkably similar including substrate specificities. Both enzymes converted delta- and gamma- into beta- and alpha-tocopherol, respectively, but beta-tocopherol was not accepted as a substrate, pointing to a specific methylation at the C(5)-position of the tocopherol aromatic head group. A kinetic analysis performed with the Arabidopsis enzyme was consistent with an iso-ordered bi-bi type reaction mechanism. Our results emphasize the role of gamma-TMT in regulating the spectrum of accumulated tocopherols in plants.
authors.affiliation: Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Koch
authors.forename: Rainer
authors.initials: R
authors.lastname: Lemke
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Heise
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Mock
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000378:metabolism; D002212:Capsicum / Q000201:enzymology*; D005638:Fruit / Q000201:enzymology; D007700:Kinetics; D008780:Methyltransferases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012435:S-Adenosylhomocysteine / Q000378:metabolism / Q000494:pharmacology; D013379:Substrate Specificity; D024502:alpha-Tocopherol / Q000378:metabolism / Q000494:pharmacology; D024503:beta-Tocopherol / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins; D012435:S-Adenosylhomocysteine; D024503:beta-Tocopherol; D008780:Methyltransferases; C047259:gamma-tocopherol methyltransferase; D024502:alpha-Tocopherol