Determination of urinary 12(S)-hydroxyeicosatetraenoic acid by liquid chromatography-tandem mass spectrometry with column-switching technique: sex difference in healthy volunteers and patients with diabetes mellitus.
We developed a determination method for human urinary 12-hydroxyeicosatetraenoic acid (12-HETE) using LC-MS-MS. This method, which includes simple extraction and detection in the SRM mode, allows precise and accurate determination of 12-HETE. There was a significant sex difference in urinary 12-HETE levels. Chiral analysis of 12-HETE using LC-MS-MS with column-switching technique revealed that the major enantiomer was 12(S)-HETE. Furthermore, the urinary level in patients with diabetes mellitus (DM) was analyzed. The present in vivo findings indicate that there could be difference in production of 12(S)-HETE between genders and 12(S)-HETE may play a role in the pathogenesis of DM.
Suzuki
Naoto
N
Department of Pharmaceutical Sciences, Tohoku University Hospital, 1-1 Seiryo-machi, Aoba-ku, 980-8574, Sendai, Japan
Hishinuma
Takanori
T
Saga
Toshihide
T
Sato
Jo
J
Toyota
Takayoshi
T
Goto
Junichi
J
Mizugaki
Michinao
M
D000368:Aged; D002138:Calibration; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003920:Diabetes Mellitus / Q000652:urine*; D005260:Female; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000652:urine*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged; D012737:Sex Factors
D006893:Hydroxyeicosatetraenoic Acids