Direct screening for phosphatase activity by turnover-based capture of protein catalysts.
Betley
Jason R
JR
Krebs Institute, Department of Chemistry, University of Sheffield, Sheffield S3 7HF, UK.
Cesaro-Tadic
Sandro
S
Mekhalfia
Abdelaziz
A
Rickard
James H
JH
Denham
Hazel
H
Partridge
Lynda J
LJ
Plückthun
Andreas
A
Blackburn
G Michael
GM
D015374:Biosensing Techniques / Q000379:methods; D002384:Catalysis; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000737:chemistry
D004791:Enzyme Inhibitors; D012710:Serum Albumin, Bovine; D010744:Phosphoric Monoester Hydrolases