title: A scalable high-throughput chemical synthesizer.
abstract: A machine that employs a novel reagent delivery technique for biomolecular synthesis has been developed. This machine separates the addressing of individual synthesis sites from the actual process of reagent delivery by using masks placed over the sites. Because of this separation, this machine is both cost-effective and scalable, and thus the time required to synthesize 384 or 1536 unique biomolecules is very nearly the same. Importantly, the mask design allows scaling of the number of synthesis sites without the addition of new valving. Physical and biological comparisons between DNA made on a commercially available synthesizer and this unit show that it produces DNA of similar quality.
authors.affiliation: Center for Biomedical Inventions (CBI), University of Texas Southwestern Medical Center, Dallas, Texas 75390-9187, USA. Eric.Livesay@UTSouthwestern.edu
authors.forename: Eric A
authors.initials: EA
authors.lastname: Livesay
authors.forename: Ying-Horng
authors.initials: YH
authors.lastname: Liu
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Luebke
authors.forename: Joel
authors.initials: J
authors.lastname: Irick
authors.forename: Yuri
authors.initials: Y
authors.lastname: Belosludtsev
authors.forename: Simon
authors.initials: S
authors.lastname: Rayner
authors.forename: Robert
authors.initials: R
authors.lastname: Balog
authors.forename: Stephen Albert
authors.initials: SA
authors.lastname: Johnston
mesh_terms: D004247:DNA / Q000096:biosynthesis / Q000138:chemical synthesis* / Q000191:economics; D019020:Directed Molecular Evolution / Q000191:economics / Q000295:instrumentation* / Q000379:methods*; D007202:Indicators and Reagents; D015345:Oligonucleotide Probes / Q000096:biosynthesis / Q000138:chemical synthesis / Q000191:economics; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000191:economics / Q000295:instrumentation / Q000379:methods; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D007202:Indicators and Reagents; D015345:Oligonucleotide Probes; D004247:DNA