title: Improved islet yields from Macaca nemestrina and marginal human pancreata after two-layer method preservation and endogenous trypsin inhibition.
abstract: We tested whether two-layer method (TLM) pancreas preservation and trypsin inhibition (Pefabloc) during processing allows longer preservation while retaining or improving viable islet recovery. Non-marginal primate (Macaca nemestrina) and marginal human (ischemic or preservation-injured) pancreata were processed with a research-oriented pan technique (Seattle method). Organs were processed upon arrival (+/- Pefabloc), or after TLM or University of Wisconsin solution (UW) preservation (+ Pefabloc). Islet yield, viability, and function were assessed. Pefabloc increased M. nemestrina islet yields from 9696 +/- 1749 IE/g to 15 822 +/- 1332 IE/g (p < 0.01). Two-layer method preservation (< 6 h) further increased yields, to 23 769 +/- 2773 IE/g (vs. + Pefabloc; p < 0.01). Similarly, Pefabloc increased marginal human islet yields from 2473 +/- 472 IE/g to 4723 +/- 1006 IE/g (p < 0.04). This increase was maintained after lengthy TLM preservation (> 30 h; 4801 +/- 1066 IE/g). We also tested the applicability of TLM preservation (23.5 +/- 3.2 h) to the processing of marginal human pancreata by the Edmonton/Immune Tolerance Network clinical protocol. Islet yield and function approached published results of pancreata processed 4.8 +/- 0.8 h after organ recovery (p = 0.06). Pefabloc, and TLM vs. UW preservation, prolonged the tolerable interval between organ recovery and islet isolation. Islet yield, viability, and functionality improved from both marginal and nonmarginal pancreata.
authors.affiliation: Puget Sound Blood Center/North-west Tissue Center, Seattle, WA, USA.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Theodore H
authors.initials: TH
authors.lastname: Rigley
authors.forename: Jo Anna
authors.initials: JA
authors.lastname: Reems
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kuroda
authors.forename: R Brian
authors.initials: RB
authors.lastname: Stevens
mesh_terms: D000818:Animals; D006801:Humans; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008254:Macaca nemestrina; D010100:Oxygen / Q000378:metabolism*; D013450:Sulfones / Q000378:metabolism*; D014021:Tissue Preservation / Q000379:methods; D014183:Transplantation, Heterologous; D014357:Trypsin; D014361:Trypsin Inhibitors
chemical_list: D013450:Sulfones; D014361:Trypsin Inhibitors; C002010:4-(2-aminoethyl)benzenesulfonylfluoride; D014357:Trypsin; D010100:Oxygen