Effect of supranutritional level of dietary alpha-tocopheryl acetate and selenium on rabbit semen.
This research examined the effects of dietary alpha-tocopheryl acetate (50 or 200 mg/kg diet) and selenium (Se, 0 or 0.5 ppm) supplementation on motion characteristics, oxidative stability and fertilizing ability of rabbit spermatozoa, fresh and stored for 24 h at 5 degrees C. The higher amount of dietary alpha-tocopheryl acetate increased the level of Vitamin E in the fresh semen (1.75 mmol/l versus 0.95 mmol/l) and its oxidative stability (thiobarbituric acid reactive substances-TBARS 12.44 nmol malondialdehyde/10(8) sperm versus 21.4 nmol malondialdehyde/10(8) sperm). Dietary Se increased gluthatione peroxidase activity (GPx) in erythrocytes (285 U/g Hb versus 207 U/g Hb), seminal plasma (270 U/l versus 190 U/l) and spermatozoa (1338 mU/10(9) sperm versus 1103 mU/10(9) sperm), whereas it did not show any effect on alpha-tocopherol level and TBARS. No synergy between Vitamin E and Se was shown. Storage for 24 h at 5 degrees C increased the TBARS level in all the experimental groups. Neither live and acrosome reacted spermatozoa, nor kinetic parameters, nor fertility rate were modified by dietary supplementation.
Castellini
Cesare
C
Department of Animal Science, Borgo 20 Giugno, 74-06100 Perugia, Italy. cesare@unipg.it
Lattaioli
Paolo
P
Bosco
Alessandro Dal
AD
Beghelli
Daniela
D
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D004032:Diet; D004357:Drug Synergism; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D005306:Fertilization; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D010084:Oxidation-Reduction; D011817:Rabbits; D012643:Selenium / Q000008:administration & dosage*; D012661:Semen / Q000737:chemistry / Q000187:drug effects*; D012662:Semen Preservation; D013094:Spermatozoa / Q000187:drug effects / Q000201:enzymology / Q000502:physiology; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D024505:Tocopherols; D014810:Vitamin E / Q000032:analysis / Q000097:blood; D024502:alpha-Tocopherol / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000032:analysis
D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D024502:alpha-Tocopherol; D012643:Selenium; D024505:Tocopherols