Determination of lomefloxacin by terbium sensitized chemiluminescence method.
A simple, rapid and sensitive chemiluminescence (CL) method was proposed for the determination of lomefloxacin (LFX). This method is based on the fact that the weak CL from the redox reaction of Ce(4+)-Na(2)SO(3) can be greatly enhanced by the complex of Tb(3+)-LFX. The CL intensity is directly proportional to the concentration of LFX in the range 2.0x10(-9) to 1.0x10(-5) mol L(-1), and the detection limit (S/N=3) is 1.1x10(-9) mol L(-1). This method has been applied to the detection of LFX in pharmaceutical preparation, urine and serum samples. Recoveries were in the range 95-105%. The CL mechanism of Ce(4+)-Na(2)SO(3)-Tb(3+)-LFX system was proposed to be an intermolecular energy transfer from excited SO(2)(*) to LFX and an intramolecular energy transfer from LFX to Tb(3+).
Nie
Li-Hua
LH
Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Zhao
Hui-Chun
HC
Wang
Xu
X
Yi
Lin
L
Lu
Yan
Y
Jin
Lin-Pei
LP
Ma
Hui-Min
HM
D000890:Anti-Infective Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D024841:Fluoroquinolones; D006801:Humans; D007700:Kinetics; D008163:Luminescent Measurements; D015363:Quinolones / Q000032:analysis* / Q000097:blood / Q000652:urine; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000295:instrumentation* / Q000379:methods*; D013725:Terbium / Q000737:chemistry*; D013997:Time Factors
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; D013725:Terbium; C053091:lomefloxacin