Localisation and regulation of the eIF4E-binding protein 4E-BP3.
The cap-binding protein eIF4E-binding protein 3 (4E-BP3) was identified some years ago, but its properties have not been investigated in detail. In this report, we investigated the regulation and localisation of 4E-BP3. We show that 4E-BP3 is present in the nucleus as well as in the cytoplasm in primary T cells, HEK293 cells and HeLa cells. 4E-BP3 was associated with eIF4E in both cell compartments. Furthermore, 4E-BP3/eIF4E association in the cytoplasm was regulated by serum or interleukin-2 starvation in the different cell types. Rapamycin did not affect the association of eIF4E with 4E-BP3 in the cytoplasm or in the nucleus.
Kleijn
Miranda
M
Division of Molecular Physiology, School of Life Sciences, University of Dundee, DD1 5EH, Dundee, UK. m.scheperkleijn@dundee.ac.uk
Scheper
Gert C
GC
Wilson
Mary L
ML
Tee
Andrew R
AR
Proud
Christopher G
CG
D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D039561:Eukaryotic Initiation Factor-4E / Q000737:chemistry* / Q000378:metabolism; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007376:Interleukin-2 / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D012314:RNA Cap Analogs / Q000378:metabolism; D020123:Sirolimus / Q000494:pharmacology; D013601:T-Lymphocytes / Q000378:metabolism; D014162:Transfection
D002352:Carrier Proteins; D016895:Culture Media, Serum-Free; C112661:EIF4EBP3 protein, human; D039561:Eukaryotic Initiation Factor-4E; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D010455:Peptides; D012314:RNA Cap Analogs; C017516:7-methylguanosine triphosphate; D020123:Sirolimus