Protein histidine phosphatase: a novel enzyme with potency for neuronal signaling.
The importance of reversible phosphorylation for neuronal signaling and cell survival is well recognized. Knowledge in vertebrates, however, is so far limited to O-phosphates from serine, threonine, and tyrosine. The authors describe an enzyme acting on N-phosphates. It is the first protein histidine phosphatase identified in vertebrates. This histidine phosphatase is ubiquitously expressed in mammalian tissues including brain. Characterization and sequencing showed a yet unknown protein with no similarity to other phosphatases. In Caenorhabditis elegans, the homolog of this histidine phosphatase was exclusively expressed in neurons, suggesting a distinct role of reversible histidine phosphorylation in neuronal functions.
Klumpp
Susanne
S
Institute for Medicinal Chemistry, Westfälische Wilhelms-University, Münster, Germany. klumpp@uni-muenster.de
Hermesmeier
Jan
J
Selke
Dagmar
D
Baumeister
Ralf
R
Kellner
Roland
R
Krieglstein
Josef
J
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology; D017173:Caenorhabditis elegans; D049452:Green Fluorescent Proteins; D006639:Histidine / Q000378:metabolism; D007202:Indicators and Reagents / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D008322:Mammals; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000201:enzymology*; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D015398:Signal Transduction / Q000502:physiology*
D007202:Indicators and Reagents; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D006639:Histidine; D010749:Phosphoprotein Phosphatases