Effect of chitosan film containing basic fibroblast growth factor on wound healing in genetically diabetic mice.
Basic fibroblast growth factor (bFGF) has been shown to stimulate wound healing. However, consistent delivery of bFGF has been problematic. We studied the stability of bFGF incorporated into a chitosan film as a delivery vehicle for providing sustained release of bFGF. The therapeutic effect of this system on wound healing in genetically diabetic mice was determined as a model for treating clinically impaired wound healing. A chitosan film was prepared by freeze-drying hydroxypropylchitosan (a water-soluble derivative of chitosan) acetate buffer solution. Growth factor was incorporated into films before drying by mixing bFGF solution with the hydroxypropylchitosan solution. bFGF activity remained stable for 21 days at 5 degrees C, and 86.2% of activity remained with storage at 25 degrees C. Full-thickness wounds were created on the backs of diabetic mice, and chitosan film or bFGF-chitosan film was applied to the wound. The wound was smaller in after 5 days in both groups, but the wound was smaller on day 20 only in the bFGF-chitosan group. Proliferation of fibroblasts and an increase in the number of capillaries were observed in both groups, but granulation tissue was more abundant in the bFGF-chitosan group. These results suggest that chitosan itself facilitates wound repair and that bFGF incorporated into chitosan film is a stabile delivery vehicle for accelerating wound healing.
Mizuno
Keisuke
K
First Department of Surgery, Nagoya University School of Medicine, Nagoya University School of Medicine, Tsuruma-cho, Showa-ku, Nagoya, 466-8550 Japan. keiko-y@med.nagoya-u.ac.jp
Yamamura
Keiko
K
Yano
Kohji
K
Osada
Takashi
T
Saeki
Satomi
S
Takimoto
Norio
N
Sakurai
Tsunehisa
T
Nimura
Yuji
Y
D000818:Animals; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2; D002686:Chitin; D048271:Chitosan