Quercetin glucuronides inhibited 2-aminofluorene acetylation in human acute myeloid HL-60 leukemia cells.
Our earlier study has demonstrated that following the exposure of rat to the arylamine carcinogen 2-aminofluorene, DNA-2-aminofluorene adducts were found in the target tissues liver, bladder, colon, lung and also in circulating leukocytes (lymphocytes and monocytes). The result also demonstrated that orally treated antioxidants decreased N-acetylation of 2-aminofluorene in target tissues and leukocytes. Therefore, this study investigated whether quercetin glucuronides could affect N-acetylation of 2-aminofluorene in human acute myeloid leukemia HL-60 cells. Evidence is presented here that human leukemia cells are capable of acetylating 2-aminofluorene. Quercetin glucuronides did inhibit 2-aminofluorene acetylation in intact cells. The results also indicated that quercetin glucuronides induced cytotoxicity in dose-dependent manner in the examined human acute myeloid leukemia HL-60 cells.
Kuo
H M
HM
Department of Parasitology, China Medical College, Taichung, Taiwan, Republic of China.
Ho
H J
HJ
Chao
P D L
PD
Chung
J G
JG
D000107:Acetylation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005449:Fluorenes / Q000378:metabolism*; D020719:Glucuronides / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D011794:Quercetin / Q000494:pharmacology*
D005449:Fluorenes; D020719:Glucuronides; C012177:2-aminofluorene; D011794:Quercetin; D004121:Dimethyl Sulfoxide