title: Detection of specific Helicobacter pylori DNA and antigens in stool samples in dyspeptic patients and healthy subjects.
abstract: In this study stool samples from dyspeptic patients and healthy subjects were used for detection of specific Helicobacter pylori antigens and DNA by immunoenzymatic test (PPHpSA) and semi-nested PCR (ureA-PCR), respectively. The H. pylori status was estimated by invasive endoscopy-based rapid urease test and histology or noninvasive urea breath test (UBT), and by serology (ELISA, Western blot). The coincidence of H. pylori-negative invasive tests or UBT and negative antigen or DNA stool tests was very high (mean 95%). The PPHpSA results were found positive for 56% and ureA-PCR for 26% of individuals with H. pylori infection confirmed by invasive tests or UBT. The detection of specific H. pylori antigens and especially DNA in feces is not sufficient as a one-step diagnosis of H. pylori infection.
authors.affiliation: Department of Immunology and Infectious Biology, University of Lódź, 90-237 Lódź, Banacha 12, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Wiśniewska
authors.forename: Hans Olof
authors.initials: HO
authors.lastname: Nilsson
authors.forename: Leokadia
authors.initials: L
authors.lastname: Bak-Romaniszyn
authors.forename: Tomasz
authors.initials: T
authors.lastname: Rechciński
authors.forename: Władysław
authors.initials: W
authors.lastname: Bielański
authors.forename: Izabela
authors.initials: I
authors.lastname: Płaneta-Małecka
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Płonka
authors.forename: Stanisław
authors.initials: S
authors.lastname: Konturek
authors.forename: Torkel
authors.initials: T
authors.lastname: Wadström
authors.forename: Wiesława
authors.initials: W
authors.lastname: Rudnicka
authors.forename: Magdalena
authors.initials: M
authors.lastname: Chmiela
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000032:analysis*; D015153:Blotting, Western; D001944:Breath Tests; D002648:Child; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004415:Dyspepsia / Q000175:diagnosis / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology*; D016481:Helicobacter Infections / Q000175:diagnosis / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004269:DNA, Bacterial