title: Microbial cell-surface display.
abstract: Cell-surface display allows peptides and proteins to be displayed on the surface of microbial cells by fusing them with the anchoring motifs. The protein to be displayed - the passenger protein - can be fused to an anchoring motif - the carrier protein - by N-terminal fusion, C-terminal fusion or sandwich fusion. The characteristics of carrier protein, passenger protein and host cell, and fusion method all affect the efficiency of surface display of proteins. Microbial cell-surface display has many potential applications, including live vaccine development, peptide library screening, bioconversion using whole cell biocatalyst and bioadsorption.
authors.affiliation: Metabolic and Biomolecular Engineering National Research Laboratory, Daejeon, South Korea. leesy@mail.kaist.ac.kr
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Choi
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Xu
mesh_terms: D000327:Adsorption; D020816:Amino Acid Motifs; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D002462:Cell Membrane / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005821:Genetic Techniques; D005822:Genetic Vectors; D006090:Gram-Negative Bacteria / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D011993:Recombinant Fusion Proteins