title: Interference-free biosensor based on screen-printing technology and sol-gel immobilization for determination of acetaldehyde in wine.
abstract: A monoenzymatic amperometric biosensor was developed for the detection of acetaldehyde. The sensor is based on the association of screen-printed carbon electrodes and aldehyde dehydrogenase immobilized by a sol-gel entrapment method. Modification of screen-printed carbon electrodes with Reinecke salt of Meldola's Blue (MBRS) resulted in highly sensitive and interference-free nicotinamide-adenine dinucleotide (NADH) detectors. Based on MBRS-mediated oxidation of NADH at -150 mV versus pseudo Ag/AgCl, acetaldehyde was determined in the range 10-260 microM, compatible with wine quality monitoring. The method of immobilization based on sol-gel entrapment was optimized to obtain the best compromise between sensitivity and operational stability. The sensor response was stable for 40 consecutive assays with methyltrimethoxysilane used as alkoxide precursor, thus allowing a possible calibration of the sensor before each measurement. The biosensors were used to analyze French wines. The method was validated with a commercially available enzymatic kit based on a standard spectrophotometric method.
authors.affiliation: Université de Perpignan, Centre de Phytopharmacie, UMR CNRS 5054, 52, Perpignan, France. noguer@univ-perp.fr
authors.forename: Thierry
authors.initials: T
authors.lastname: Noguer
authors.forename: Anca
authors.initials: A
authors.lastname: Tencaliec
authors.forename: Carole
authors.initials: C
authors.lastname: Calas-Blanchard
authors.forename: Alina
authors.initials: A
authors.lastname: Avramescu
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Marty
mesh_terms: D000079:Acetaldehyde / Q000032:analysis*; D000444:Aldehyde Dehydrogenase / Q000737:chemistry; D015374:Biosensing Techniques; D002138:Calibration; D004566:Electrodes; D004800:Enzymes, Immobilized; D005782:Gels; D007202:Indicators and Reagents; D009243:NAD / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011199:Potentiometry; D015203:Reproducibility of Results; D014159:Transducers; D014920:Wine / Q000032:analysis*
chemical_list: D004800:Enzymes, Immobilized; D005782:Gels; D007202:Indicators and Reagents; D009243:NAD; D011092:Polyethylene Glycols; D000444:Aldehyde Dehydrogenase; D000079:Acetaldehyde