Phylogeny of ruminants secretory ribonuclease gene sequences of pronghorn (Antilocapra americana).
Phylogenetic analyses based on primary structures of mammalian ribonucleases, indicated that three homologous enzymes (pancreatic, seminal and brain ribonucleases) present in the bovine species are the results of gene duplication events, which occurred in the ancestor of the ruminants after divergence from other artiodactyls. In this paper sequences are presented of genes encoding pancreatic and brain-type ribonuclease genes of pronghorn (Antilocapra americana). The seminal-type ribonuclease gene could not be detected in this species, neither by PCR amplification nor by Southern blot analyses, indicating that it may be deleted completely in this species. Previously we demonstrated of a study of amino acid sequences of pancreatic ribonucleases of a large number of ruminants the monophyly of bovids and cervids, and that pronghorn groups with giraffe. Here we present phylogenetic analyses of nucleotide sequences of ribonucleases and other molecules from ruminant species and compare these with published data. Chevrotain (Tragulus) always groups with the other ruminants as separate taxon from the pecora or true ruminants. Within the pecora the relationships between Bovidae, Cervidae, Giraffidae, and pronghorn (Antilocapra) cannot be decided with certainty, although in the majority of analyses Antilocapra diverges first, separately or joined with giraffe. Broad taxon sampling and investigation of specific sequence features may be as important for reliable conclusions in phylogeny as the lengths of analyzed sequences.
Beintema
Jaap J
JJ
Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. beintema@chem.rug.nl
Breukelman
Heleen J
HJ
Dubois
Jean-Yves F
JY
Warmels
Hayo W
HW
D000595:Amino Acid Sequence; D000818:Animals; D000866:Antelopes / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D019143:Evolution, Molecular; D016013:Likelihood Functions; D008297:Male; D008969:Molecular Sequence Data; D010179:Pancreas / Q000378:metabolism; D010802:Phylogeny; D011328:Prions / Q000235:genetics; D012260:Ribonucleases / Q000235:genetics* / Q000378:metabolism; D012418:Ruminants / Q000235:genetics / Q000378:metabolism / Q000821:virology; D012661:Semen / Q000378:metabolism
D011328:Prions; D012260:Ribonucleases