Identification and characterization of P15RS, a novel P15(INK4b) related gene on G1/S progression.
To screen genes involved in P15(INK4b) regulation during cell cycle, differential display method was applied to compare mRNAs from G(1) synchronized cells of MLIK6, which overexpressed P15(INK4b) gene, and its control MLC2. By using this approach, 15 cDNA fragments that were preferentially expressed in MLIK6 cells, but not in MLC2 cells, were screened out. A novel gene named P15RS was identified with further analysis. Combining the sequence from DD-PCR, homology analysis against EST database and RACE, a 4,404 bp complete cDNA sequence of P15RS was generated. Sequence analysis revealed that P15RS cDNA encoded a 312-amino-acid peptide containing a RAR domain that is involved in regulation of nuclear pre-mRNA, which suggests that P15RS may be a nuclear regulation protein. Genomic sequence analysis demonstrated that human P15RS gene was localized on chromosome 18q12 with seven exons and six introns. Expressing antisense P15RS in MLIK6 cells can up-regulate the expression of cyclinD1 and cyclinE. These data indicate that P15RS may act as a negative regulator in G(1) phase.
Liu
Jun
J
The Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Science, Beijing Normal University, Beijing 100875, China. liuhuitu@bnu.edu.cn
Liu
Huitu
H
Zhang
Xue
X
Gao
Ping
P
Wang
Juan
J
Hu
Zhijun
Z
D000595:Amino Acid Sequence; D016375:Antisense Elements (Genetics); D001483:Base Sequence; D015152:Blotting, Northern; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D018076:DNA, Complementary / Q000096:biosynthesis / Q000302:isolation & purification; D016193:G1 Phase; D040481:Genome Components; D006801:Humans; D008969:Molecular Sequence Data; D012097:Repressor Proteins; D016196:S Phase; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
D016375:Antisense Elements (Genetics); C493966:CDKN2B protein, human; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16; D018076:DNA, Complementary; C470426:RPRD1A protein, human; D012097:Repressor Proteins; D025521:Tumor Suppressor Proteins