The anti-proliferative gene TIS21 is involved in osteoclast differentiation.
The remodeling process of bone is accompanied by complex changes in the expression levels of various genes. Several approaches have been employed to detect differentially-expressed genes in regard to osteoclast differentiation. In order to identify the genes that are involved in osteoclast differentiation, we used a cDNAarray-nylon membrane. Among 1,200 genes that showed a measurable signal, 19 genes were chosen for further study. Eleven genes were up-regulated; eight genes were downregulated. TIS21 was one of the up-regulated genes which were highly expressed in mature osteoclasts. To verify the cDNA microarray results, we carried out RT-PCR and real-time RT-PCR for the TIS21 gene. The TIS21 mRNA level was higher in differentiated-osteoclasts when compared to undifferentiated bone-marrow macrophages. Furthermore, the treatment with 1 mM of a TIS21 antisense oligonucleotide reduced the formation of osteoclasts from the bone-marrow-precursor cells by approximately 30%. These results provide evidence for the potential role of TIS21 in the differentiation of osteoclasts
Lee
Soo Woong
SW
National Research Laboratory for Bone Metabolism, Research Center for Proteineous Materials, Chosun University, Gwangju.
Kwak
Han Bok
HB
Lee
Hong Chan
HC
Lee
Seung Ku
SK
Kim
Hong-Hee
HH
Lee
Zang Hee
ZH
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation; D016147:Genes, Tumor Suppressor; D017874:Immediate-Early Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010010:Osteoclasts / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins; D015854:Up-Regulation
C489435:Btg2 protein, mouse; D018076:DNA, Complementary; D017874:Immediate-Early Proteins; D016376:Oligonucleotides, Antisense; D025521:Tumor Suppressor Proteins