[Evaluation of an ELISA kit made in our laboratory for intratypic discrimination of poliovirus type 1 strains isolated from acute flask paralysis].
In this study, we aimed to develop and evaluate an enzyme based immunological method (ELISA) for the intratypic differentiation (whether if Sabin like-SL, or non Sabin like/wild type-NSL) of poliovirus type 1 isolates, in the frame of Polio Eradication Programme (PEP) in Turkey. Five poliovirus type 1 strains which were formerly isolated and identified in our laboratory from fecal samples of acute flask paralysis (AFP) patients, and standard reference strains of vaccine type 1 and prototype wild (Mahoney) polioviruses have been tested into plate wells that bounded with 5 different monoclonal antibodies (mAbs B18, S2-36, M18, B82, M49). The wells which gave high absorbance than cutoff value estimated by comparing negative control wells, were accepted as positive. As a result, the standard poliovirus strains were differentiated correctly, whereas 2 patient isolates were identified as SL and 3 were NSL. In conclusion, our home-made ELISA method found to be successful for intratypic differentiation of poliovirus type 1 isolates, however more detailed studies are necessary in order to increase the susceptibility and specificity of this test.
Ozkaya
Etem
E
Refik Saydam Hifzissihha Merkezi Başkanliği, Viroloji, Doku Kültürü ve Enterovirus Laboratuvari, Ankara.
Arita
Mineo
M
D000208:Acute Disease; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D005243:Feces / Q000821:virology; D006801:Humans; D010243:Paralysis / Q000821:virology*; D011051:Poliomyelitis / Q000821:virology*; D017955:Poliovirus / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D012015:Reference Standards; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal