title: Single-step PCR optimization using touchdown and stepdown PCR programming.
authors.affiliation: Department of Biological Science, Florida State University, Tallahassee, FL, USA.
authors.forename: Kenneth H
authors.initials: KH
authors.lastname: Roux
mesh_terms: D017931:DNA Primers; D016133:Polymerase Chain Reaction / Q000379:methods*; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers