title: [Effects of newborn bull serum and vitamins on cryopreservation of mouse seminiferous epithelial cells].
abstract: OBJECTIVE
  To investigate the effects of newborn bull serum(NBS), vitamin C and vitamin E on cryopreservation of mouse seminiferous epithelial cells.
  
  
  METHODS
  The seminiferous epithelial cells from 7-day-old mice were cryopreserved in different freezing solutions. The cell recoveries were examined by Trypan blue exclusive staining after thawing. The freezing solutions composed of DMEM, 10% dimethylsulphoxide(DMSO), and 0, 5%, 10%, or 20% NBS, respectively, or composed of DMEM, 10% DMSO, 10% NBS, and 150 micrograms/ml vitamin C or 50 micrograms/ml vitamin E, respectively.
  
  
  RESULTS
  The cell recoveries in freezing solution containing 0, 5%, 10%, or 20% NBS were 83.4%, 84.7%, 85.7% and 83.6%, respectively. There were no significant differences between them. The cell recoveries in freezing solution containing vitamin C or vitamin E were 88.0% and 82.9%, respectively. There was no significant differences compared with that in freezing solution containing 10% DMSO and 10% NBS.
  
  
  CONCLUSIONS
  NBS, vitamin C and vitamin E have no significant protecting effects on mouse seminiferous epithelial cells, and can not significantly improve the cell recoveries.
authors.affiliation: Department of Preclinical Veterinary Medicine, Faculty of Animal Science and Technology, Quartermaster University of PLA, Changchun, Jilin 130062, China. llian63@sohu.com
authors.forename: Lian-Jun
authors.initials: LJ
authors.lastname: Li
authors.forename: De-Xue
authors.initials: DX
authors.lastname: Li
authors.forename: Xue-Ming
authors.initials: XM
authors.lastname: Zhang
authors.forename: Zhan-Peng
authors.initials: ZP
authors.lastname: Yue
authors.forename: Xing-Hao
authors.initials: XH
authors.lastname: Wen
authors.forename: Bing-Kun
authors.initials: BK
authors.lastname: Luo
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002417:Cattle; D015925:Cryopreservation; D004847:Epithelial Cells / Q000502:physiology; D005312:Fetal Blood / Q000502:physiology*; D008297:Male; D051379:Mice; D012670:Seminiferous Epithelium / Q000166:cytology*; D014812:Vitamin K / Q000494:pharmacology*
chemical_list: D014812:Vitamin K; D001205:Ascorbic Acid