The coenzyme thiamine pyrophosphate inhibits the self-splicing of the group I intron.
Effects of the coenzyme thiamine pyrophosphate and its analogs on the inhibition of self-splicing of primary transcripts of the phage T4 thymidylate synthase gene (td) were investigated. Of all compounds tested, the coenzyme thiamine pyrophosphate was the most potent inhibitor and the order of inhibitory efficiency for compounds tested was as follows: thiamine pyrophosphate>thiamine monophosphate>thiamine>thiochrome. Increasing guanosine concentration overcame the suppression of self-splicing by thiamine pyrophosphate close to the level of normal splicing. Kinetic analysis demonstrated that thiamine pyrophosphate acts as a competitive inhibitor for the td intron RNA with a Ki of 2.2mM. The splicing specificity inhibition by thiamine pyrophosphate is predominantly due to changes in Km.
Ahn
Sung Joon
SJ
Department of Biology, Dongguk University, 100-715, Seoul, South Korea.
Park
In Kook
IK
D017122:Bacteriophage T4 / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D006151:Guanosine / Q000378:metabolism / Q000494:pharmacology; D007438:Introns; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology; D012326:RNA Splicing / Q000187:drug effects*; D016337:RNA, Catalytic / Q000187:drug effects; D013831:Thiamine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D013833:Thiamine Monophosphate / Q000378:metabolism / Q000494:pharmacology; D013835:Thiamine Pyrophosphate / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D013940:Thymidylate Synthase / Q000235:genetics
D016337:RNA, Catalytic; D006151:Guanosine; D013833:Thiamine Monophosphate; C005793:thiochrome; D013940:Thymidylate Synthase; D008274:Magnesium; D013835:Thiamine Pyrophosphate; D013831:Thiamine