title: The cartilage-specific fibronectin isoform has a high affinity binding site for the small proteoglycan decorin.
abstract: Binding of fibronectin to the small proteoglycan decorin plays an important role in cell differentiation and cell migration. The cartilage-specific (V+C)(-) fibronectin isoform, in which nucleotides that normally encode the protein segments V, III(15), and I(10) are spliced out, is one of the major splice variants present in cartilage matrices. Full-length and truncated cDNA constructs were used to express recombinant versions of fibronectin. Results demonstrated that the (V+C)(-) isoform has a higher affinity for decorin. Dissociation constants for decorin and fibronectin interaction were calculated to be 93 nm for the V(+)C(+) isoform and 24 nm and 223 nm for (V+C)(-) fibronectin. Because heparin competed with decorin competitively, binding of decorin to fibronectin likely occurs at a heparin-binding region. We propose that alternative splicing of the V and C regions changes the global conformation of fibronectin in such a way that it opens an additional decorin-binding site. This conformational change is responsible for the higher affinity of the (V+C)(-) fibronectin isoform for decorin.
authors.affiliation: James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
authors.forename: Rina
authors.initials: R
authors.lastname: Gendelman
authors.forename: Nancy I
authors.initials: NI
authors.lastname: Burton-Wurster
authors.forename: James N
authors.initials: JN
authors.lastname: MacLeod
authors.forename: George
authors.initials: G
authors.lastname: Lust
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002358:Cartilage, Articular / Q000378:metabolism*; D017931:DNA Primers; D058575:Decorin; D004285:Dogs; D016326:Extracellular Matrix Proteins; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism*; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D020033:Protein Isoforms; D011509:Proteoglycans