Profiling of gene expression associated with hepatolithiasis by complementary DNA expression array.
By use of cDNA expression array method, we compared the expression profiles of genes in hepatolithiasis tissues with those of paired normal tissues. Expression of 1176 cancer related genes that include 33 tumor suppressor genes, 100 proto-oncogenes and 37 DNA damage repair genes were examined in this study. We found that expression of tumor suppressor genes and proto-oncogenes was systemically activated in hepatholithiasis tissues, suggesting that expression of genes controlling cell growth becomes unstable in liver lobe of hepatolithiasis. In contrast, expression of DNA damage repair genes were not activated but rather remained unchanged in hepatholithiasis tissues, suggesting that repair process is not strongly activated but rather impaired in hepatholithiasis tissues.
Kokuryo
Toshio
T
Division of Surgical Oncology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
Yamamoto
Tatsuyoshi
T
Oda
Koji
K
Kamiya
Junichi
J
Nimura
Yuji
Y
Senga
Takeshi
T
Yasuda
Yoshinari
Y
Ohno
Yoshiyuki
Y
Nakanuma
Yasuni
Y
Chen
Miin-Fu
MF
Jan
Yi-Yin
YY
Yeh
Ta-Sen
TS
Chiu
Cheng-Taug
CT
Hsieh
Ling-Ling
LL
Hamaguchi
Michinari
M
D002137:Calculi / Q000150:complications / Q000235:genetics*; D018281:Cholangiocarcinoma / Q000209:etiology; D004249:DNA Damage; D020869:Gene Expression Profiling; D016147:Genes, Tumor Suppressor; D006801:Humans; D008099:Liver / Q000378:metabolism; D008107:Liver Diseases / Q000150:complications / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011519:Proto-Oncogenes