title: Effect of retinoblastoma gene on human osteosarcoma cell line OS732.
abstract: OBJECTIVE
  To investigate the effect of growth suppression and gap junctional intercellular communication (GJIC) on osteosarcoma cell line OS732 transduced with the exogenous N-terminal truncated retinoblastoma (Rb) gene.
  
  
  METHODS
  Recombinant eukaryotic expression plasmid harbouring 1.65 kb Rb gene was constructed and transduced into OS732 cells. Rb mRNA expression was detected by RT-PCR and Northern blot. The cell count, cell cycle analysis and soft agar colony formation test were used to observe growth features. RT-PCR and Lucifer yellow dye transfer were carried out to measure GJIC.
  
  
  RESULTS
  Both exogenous and endogenous Rb gene could be detected in OS732 cell transduced with the N-terminal truncated Rb gene. Compared with the parental cells, change in morphology of Rb-transduced cells were found; growth rate decreased; amount of colony formation reduced; cell cycle arrested at G(0)/G(1) checkpoint; Connexin43 expression and GJIC enhanced.
  
  
  CONCLUSIONS
  The malignant phenotype of OS732 is inhibited and GJIC is increased by transduction of N-terminal truncated Rb gene.
authors.affiliation: Department of Pathology, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China (Email: qiaohui99@163.net)
authors.forename: Hui
authors.initials: H
authors.lastname: Qiao
authors.forename: Jushi
authors.initials: J
authors.lastname: Qiu
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
mesh_terms: D001859:Bone Neoplasms; D002450:Cell Communication; D002460:Cell Line; D016161:Genes, Retinoblastoma; D006801:Humans; D012516:Osteosarcoma; D014407:Tumor Cells, Cultured