title: Comparison of levels of HIV-1 resistance to protease inhibitors by recombinant versus conventional virus phenotypic assay and two genotypic interpretation procedures in treatment-naive and HAART-experienced HIV-infected patients.
abstract: OBJECTIVE
  To compare genotypic and phenotypic HIV-1 drug resistance assays.
  
  
  METHODS
  Protease inhibitor (PI) susceptibility was phenotypically analysed in HIV-1 isolates and recombinant viruses expressing proteases from viral isolates. Two genotypic interpretation methods were carried out in parallel.
  
  
  RESULTS
  Entirely concordant resistance levels were shown in 5/10 (50%) highly active anti-retroviral therapy (HAART)-experienced patients, whereas minor discrepancies were observed in the remaining five patients.
  
  
  CONCLUSIONS
  The four assays provide comparable results. The recombinant virus phenotypic assay may provide the most accurate evaluation of resistance; however, genotypic interpretation procedures are helpful for daily therapeutic decisions.
authors.affiliation: Servizio di Virologia, IRCCS Policlinico San Matteo, 27100 Pavia, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Paolucci
authors.forename: Fausto
authors.initials: F
authors.lastname: Baldanti
authors.forename: Maurizio
authors.initials: M
authors.lastname: Zavattoni
authors.forename: Giuditta
authors.initials: G
authors.lastname: Comolli
authors.forename: Nazzarena
authors.initials: N
authors.lastname: Labò
authors.forename: Stefano
authors.initials: S
authors.lastname: Menzo
authors.forename: Massimo
authors.initials: M
authors.lastname: Clementi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Gerna
mesh_terms: D023241:Antiretroviral Therapy, Highly Active / Q000706:statistics & numerical data; D024882:Drug Resistance, Viral / Q000235:genetics*; D005838:Genotype; D015658:HIV Infections / Q000188:drug therapy* / Q000235:genetics / Q000821:virology*; D017320:HIV Protease Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D015497:HIV-1 / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D006367:HeLa Cells; D006801:Humans; D010641:Phenotype; D011995:Recombination, Genetic
chemical_list: D017320:HIV Protease Inhibitors