title: Induction of colitis in mice deficient of Peyer's patches and mesenteric lymph nodes is associated with increased disease severity and formation of colonic lymphoid patches.
abstract: Inflammatory bowel disease is associated with immune activation in Peyer's patches and mucosal lymph nodes. The role of these organs in dextran sodium sulfate (DSS)-induced colitis was investigated. We used mice lacking Peyer's patches and/or lymph nodes because of lymphotoxin-alpha gene deficiency or treatment in utero with lymphotoxin-beta-receptor IgG and tumor necrosis factor-receptor-I (55)-IgG fusion proteins. Mice lacking Peyer's patches and lymph nodes because of lymphotoxin-alpha deficiency or in utero fusion protein treatment developed more severe colitis than control mice as indicated by more severe intestinal shrinking, longer colonic ulcers, and higher histological disease scores. Oral DSS triggered the formation of colonic submucosal lymphoid patches in these mice and caused an increase in the number of submucosal lymphoid patches in mice treated in utero with the fusion proteins. Mice lacking Peyer's patches only showed more submucosal lymphoid patches whereas intestinal length and histological disease score were similar to control mice. In conclusion, more severe DSS-induced colitis correlates with the loss of the mesenteric lymph nodes. However, neither the absence of Peyer's patches nor the presence of colonic lymphoid patches were correlated with increased disease severity.
authors.affiliation: Department of Medicine B, Münster University Hospital, Albert Schweitzer-Strasse 33, D-48129 Münster, Germany. spahnth@t-online.de
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Spahn
authors.forename: Hermann
authors.initials: H
authors.lastname: Herbst
authors.forename: Paul D
authors.initials: PD
authors.lastname: Rennert
authors.forename: Norbert
authors.initials: N
authors.lastname: Lügering
authors.forename: Christian
authors.initials: C
authors.lastname: Maaser
authors.forename: Mathias
authors.initials: M
authors.lastname: Kraft
authors.forename: Adriano
authors.initials: A
authors.lastname: Fontana
authors.forename: Howard L
authors.initials: HL
authors.lastname: Weiner
authors.forename: Wolfram
authors.initials: W
authors.lastname: Domschke
authors.forename: Torsten
authors.initials: T
authors.lastname: Kucharzik
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology*; D003106:Colon / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D016264:Dextran Sulfate / Q000633:toxicity; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007202:Indicators and Reagents / Q000633:toxicity; D008198:Lymph Nodes / Q000166:cytology / Q000378:metabolism*; D008233:Lymphotoxin-alpha / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D010581:Peyer's Patches / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000494:pharmacology
chemical_list: D007202:Indicators and Reagents; D008233:Lymphotoxin-alpha; D011993:Recombinant Fusion Proteins; D016264:Dextran Sulfate