Poly(A)-binding protein and eRF3 are associated in vivo in human and Xenopus cells.
An interaction between human poly(A)-binding protein (PABP) et human eRF3 has been demonstrated using a double-hybrid approach and in vitro assays. Here, we show that the binding of both proteins is conserved through evolution. We also demonstrate that the last 39 C-terminal amino acids of PABP contain the interface that interacts with eRF3. This region includes helix 5, identified by RMN, which is conserved in all known PABPs. Lastly, we demonstrate that eRF3 et PABP molecules interact in vivo.
Cosson
Bertrand
B
Université de Rennes 1, CNRS UMR 6061, IFR 97, avenue du professeur Leon Bernard, 35043 Rennes cedex, France.
Berkova
Nadia
N
Couturier
Anne
A
Chabelskaya
Svetlana
S
Philippe
Michel
M
Zhouravleva
Galina
G
D000818:Animals; D002457:Cell Extracts; D002478:Cells, Cultured; D018388:Codon, Terminator / Q000235:genetics*; D005057:Eukaryotic Cells / Q000378:metabolism*; D019143:Evolution, Molecular; D006801:Humans; D010454:Peptide Termination Factors / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D039102:Poly(A)-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics*; D017434:Protein Structure, Tertiary / Q000235:genetics; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D017386:Sequence Homology, Amino Acid; D014982:Xenopus laevis
D002457:Cell Extracts; D018388:Codon, Terminator; D010454:Peptide Termination Factors; D039102:Poly(A)-Binding Proteins; C087971:peptide-chain-release factor 3