title: Inhibitory effect of p27KIP1 gene transfer on head and neck squamous cell carcinoma cell lines.
abstract: BACKGROUND
  Although p27 gene mutations are rarely found in cancer, the level of p27 protein expression decreases during tumor development. In several tumors, including laryngeal cancer, decreased expression of p27 is associated with a poor prognosis.
  
  
  METHODS
  The proliferation-inhibitory effect of p27 gene transfer on human head and neck squamous cell carcinoma (HNSCC) cell lines (SNU-1041, SNU-1066, and SNU-1076) by adenoviral vector was investigated.
  
  
  RESULTS
  On transduction of the human HNSCC cell line with adenovirus-p27 (Ad-p27), a high level of p27 expression and increase of cyclin D1 and E were observed. Cell cycle analysis showed a marked decrease in the proportion of cells in S phase and an increase in G1 phase. Soft agar clonogenic assay showed a marked decrease in clonogenicity.
  
  
  CONCLUSIONS
  These results suggested that overexpression of p27 could show proliferation-inhibitory effects on HNSCC cell lines. Thus, gene therapy using adenovirus-p27 seemed to have a potential to develop into a new cancer gene therapy modality.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul, 110-744, Korea.
authors.forename: Tae-Yong
authors.initials: TY
authors.lastname: Koh
authors.forename: Seok-Woo
authors.initials: SW
authors.lastname: Park
authors.forename: Kyung-Ho
authors.initials: KH
authors.lastname: Park
authors.forename: Sang-Goo
authors.initials: SG
authors.lastname: Lee
authors.forename: Jae-Goo
authors.initials: JG
authors.lastname: Seol
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Lee
authors.forename: Choon-Taek
authors.initials: CT
authors.lastname: Lee
authors.forename: Dae-Seog
authors.initials: DS
authors.lastname: Heo
authors.forename: Kwang-Hyun
authors.initials: KH
authors.lastname: Kim
authors.forename: Myung-Whun
authors.initials: MW
authors.lastname: Sung
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D017209:Apoptosis; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000628:therapy*; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D015316:Genetic Therapy; D005822:Genetic Vectors; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000628:therapy*; D006801:Humans; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases