CDKN2A region polymorphism and genetic susceptibility to melanoma in the melim swine model of familial melanoma.
Some herds of miniature swine are genetically predisposed to cutaneous melanoma. To test if swine melanoma susceptibility could be linked to the CDKN2A gene, which is involved in a proportion of 9p21-linked human familial melanoma, we performed a genetic analysis of miniature pigs of the MeLiM strain. F(1) and backcross animals were generated by crossing 1 MeLiM boar with healthy Duroc sows. We isolated the swine CDKN2A gene and characterized a linked informative microsatellite marker, the S0644 marker. Using this marker and 2 flanking markers, we analyzed the segregation of the CDKN2A gene in a 3-generation pedigree. Allelic association, linkage analysis and haplotype analysis of these data led to exclusion of the CDKN2A gene as a candidate for melanoma susceptibility. Nonetheless, this analysis suggests an association with the swine 1q25 chromosomal region, which is homologous to the human 9p21 region.
Le Chalony
Catherine
C
Laboratoire de Radiobiologie et d'Etude du Génome, Commíssariat à l'Energie Atomique-Institut National de Recherche Agronomique, Jouy-en-Josas, France. lechalon@diamant.jouy.inra.fr
Renard
Christine
C
Vincent-Naulleau
Silvia
S
Crechet
Françoise
F
Leplat
Jean-Jacques
JJ
Tricaud
Yves
Y
Horak
Vratislav
V
Gruand
Joseph
J
Le Roy
Pascale
P
Frelat
Gérard
G
Geffrotin
Claudine
C
D000483:Alleles; D000818:Animals; D020090:Chromosome Segregation; D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D019942:Genes, p16; D008040:Genetic Linkage; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D008545:Melanoma / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D018895:Microsatellite Repeats; D010375:Pedigree; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012878:Skin Neoplasms / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D013553:Swine Diseases / Q000235:genetics*; D013556:Swine, Miniature
D017931:DNA Primers; D004273:DNA, Neoplasm; D005819:Genetic Markers