Development of monoclonal antibodies against creatine kinase MB2.
An increase of creatine kinase MB (CKMB) in serum has long been used as a marker for acute myocardial infarction (AMI). It is important with an early diagnosis and since the amount of the CKMB2 isoform rises above reference levels much earlier than the total creatine kinase amount, quantification of CKMB isoforms could be a feasible alternative for early analysis. The two CKMB isoforms differ by only one C-terminal lysine residue, which makes it difficult to separate one from the other. To overcome this problem, monoclonal antibodies were produced using unique peptides as antigen in hybridoma technology. Two peptides with 16 and 15 amino acids corresponding to the C-terminal end of the M-subunits of CKMB2 and CKMB1, respectively, were conjugated to keyhole limpet hemocyanin and used as antigens. Sixteen different monoclonal antibodies to these peptides were obtained and characterized. Their specificity was analyzed by immunoassay and 10 of the antibodies showed cross-reactive binding to creatine kinase. Surface plasmon resonance based biosensor analysis was used to determine affinity and kinetics towards the peptides and the epitopes of four of the antibodies were studied by means of phage display. Some of these antibodies have binding properties that might qualify them for use in the establishment of procedures allowing early diagnosis of AMI.
Leickt
L
L
Department of Chemistry and Biomedical Sciences, University of Kalmar, Sweden.
Grubb
A
A
Ohlson
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D003402:Creatine Kinase / Q000737:chemistry / Q000276:immunology*; D052279:Creatine Kinase, MB Form; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D007527:Isoenzymes / Q000737:chemistry / Q000276:immunology*; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D020349:Surface Plasmon Resonance
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D003402:Creatine Kinase; D052279:Creatine Kinase, MB Form