title: High prevalence of Neisseria gonorrhoeae and multiple sexually transmitted diseases among rural women in the Eastern Highlands Province of Papua New Guinea, detected by polymerase chain reaction.
abstract: BACKGROUND
  In a previous community-based study among rural women in the Eastern Highlands Province (EHP) of Papua New Guinea we determined that the prevalences of Trichomonas vaginalis infection, Chlamydia trachomatis infection, and syphilis were 46%, 26%, and 4%, respectively. Surprisingly, however, the prevalence of Neisseria gonorrhoeae infection was only 1%, which we considered low in consideration of the high prevalence of other sexually transmitted diseases (STDs). The aim of the current study was to reexamine samples that were collected in that survey and retest them with use of polymerase chain reaction (PCR).
  
  
  METHODS
  Using a cluster-sampling method, we surveyed 201 women aged 15 to 45 years in a population of approximately 19,000 people. In addition, 243 other women living in the same area who wished to be screened for STDs were included in the study.
  
  
  METHODS
  Endocervical samples that were stored frozen at -80 degrees C were retested with multiplex PCR (M-PCR) for the detection of both N gonorrhoeae and C trachomatis and with a separate PCR for the detection of T vaginalis.
  
  
  RESULTS
  A total of 373 samples that were still available were analyzed. The prevalences of T vaginalis, C trachomatis, and N gonorrhoeae infections were 42.6%, 26.5%, and 18.2%, respectively; 59.8% of the women had at least one STD, while 21.7% had mixed infections, 5.9% of them with all three pathogens.
  
  
  CONCLUSIONS
  STDs are very common among rural women in the EHP of Papua New Guinea and often present as multiple infections.
authors.affiliation: Papua New Guinea Institute of Medical Research, P.O. Box 60, Goroka, EHP 441, Papua New Guinea. csmgone@datec.com.pg
authors.forename: Charles S
authors.initials: CS
authors.lastname: Mgone
authors.forename: Tony
authors.initials: T
authors.lastname: Lupiwa
authors.forename: William
authors.initials: W
authors.lastname: Yeka
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D002690:Chlamydia Infections / Q000453:epidemiology / Q000209:etiology; D005260:Female; D006069:Gonorrhea / Q000453:epidemiology* / Q000209:etiology; D006801:Humans; D008875:Middle Aged; D016017:Odds Ratio; D010219:Papua New Guinea / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012423:Rural Health; D012749:Sexually Transmitted Diseases / Q000453:epidemiology / Q000209:etiology; D013587:Syphilis / Q000453:epidemiology / Q000209:etiology; D014247:Trichomonas Vaginitis / Q000453:epidemiology / Q000209:etiology; D016387:Women's Health