title: The in vitro efficacy of a quaternary ammonia disinfectant and/or ethylenediaminetetraacetic acid-tris against commercial broiler hatchery isolates of Pseudomonas aeruginosa.
abstract: Studies have indicated variations in the degree of efficacy of certain commercial disinfectants used in poultry production facilities. We used an adequate method of in vitro testing to compare the efficacy of disinfectants while testing them in conditions similar to those of the poultry facilities. BioSentry 904, ethylenediaminetetracetic acid (EDTA)-Tris, and a combination of the two were tested by this method against five field isolates of Pseudomonas aeruginosa at 10(3), 10(6), and 10(9) colony-forming units (CFU)/ml. At the 10(9) CFU/ml concentration, most compounds failed to achieve a total kill with a contact time of 15 min. When tested at bacterial concentrations of 10(3) CFU/ml, the combination of EDTA-Tris mixed at a 1:1 ratio with BioSentry 904 killed the bacteria upon initial contact (< or = 0.05 min). This disinfectant mixture exhibited antagonistic, indifferent, or synergetic effects when exposed to different bacterial isolates at a concentration of 10(6) CFU/ml.
authors.affiliation: Department of Poultry Science, College of Agriculture and Applied Economics University of Georgia, Athens, GA 30602, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: Walker
authors.forename: J E
authors.initials: JE
authors.lastname: Sander
authors.forename: I-Hsin
authors.initials: IH
authors.lastname: Cheng
authors.forename: R E
authors.initials: RE
authors.lastname: Wooley
mesh_terms: D000818:Animals; D002645:Chickens; D004202:Disinfectants / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology*; D006799:Housing, Animal; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*
chemical_list: D004202:Disinfectants; D000644:Quaternary Ammonium Compounds; D004492:Edetic Acid