Application of centrifugal force to the extraction and separation of parasorboside and gerberin from Gerbera hybrida.
A method for the separation of parasorboside and gerberin from the ornamental plant Gerbera hybrida (Asteraceae) has been developed. The two closely related glucosides were extracted using an Extrachrom instrument, a prototype multi-functional separation tool equipped with an extraction chamber. The rotation planar extraction procedure was compared with that of a medium pressure solid-liquid extraction system. The resulting extracts were pre-purified using rotation planar chromatography and the results compared with those obtained using medium pressure liquid chromatography with silica gel as the stationary phase and a mobile phase of methanol:ethyl acetate:tetrahydrofuran at selectivity point Ps = 111 with 1% formic acid as modifier. The title compounds were isolated from the purified extracts by TLC and their structures confirmed by 1H- and 13C-NMR spectroscopy.
Yrjönen
Teijo
T
Department of Pharmacy, Division of Pharmacognosy, University of Helsinki, PO Box 56, Helsinki FIN-00014, Finland.
Vuorela
Pia
P
Klika
Karel D
KD
Pihlaja
Kalevi
K
Teeri
Teemu H
TH
Vuorela
Heikki
H
D019659:Asteraceae / Q000737:chemistry*; D002498:Centrifugation / Q000295:instrumentation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D035264:Flowers / Q000737:chemistry; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D011753:Pyrones / Q000737:chemistry* / Q000302:isolation & purification
D005960:Glucosides; D010936:Plant Extracts; D011753:Pyrones; C473204:gerberin; C473203:parasorboside