title: Molecular studies of the synergistic interactions between plum pox virus HC-Pro protein and potato virus X.
abstract: Helper component proteinase (HC-Pro) is a multifunctional viral protein involved in vection and movement of potyvirus; suppression of host post-transcriptional gene silencing reaction; and synergism of potyvirus with other viruses, notably potexvirus. When the HC-Pro of plum pox potyvirus (PPV) was transiently expressed in N. benthamiana and N. clevelandii plants via potato virus X (PVX) vector, a highly synergistic response of leaf necrosis or plant death, was observed in PVXHC-infected plants. However, when mutations were introduced into the highly conserved "PTK" and "KITC" motifs of HC-Pro, known for their involvement in protein/protein interaction during aphid-mediated transmission of potyvirus, the resulting change of amino acid residue from lysine (K) to glutamic acid (E) in the "KITC" motif had no obvious influence.
authors.affiliation: Centre de Recherches INRA-Bordeaux, Villenave d'Ornon, France.
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.forename: M
authors.initials: M
authors.lastname: Ravelonandro
mesh_terms: D019943:Amino Acid Substitution; D003546:Cysteine Endopeptidases / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D005822:Genetic Vectors; D018698:Glutamic Acid; D008239:Lysine; D009154:Mutation; D010935:Plant Diseases / Q000821:virology; D017801:Plum Pox Virus / Q000235:genetics / Q000502:physiology*; D017863:Potexvirus / Q000235:genetics / Q000472:pathogenicity*; D011485:Protein Binding; D016865:Reassortant Viruses / Q000472:pathogenicity*; D014026:Nicotiana / Q000235:genetics / Q000821:virology; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D014774:Virulence
chemical_list: D014764:Viral Proteins; D018698:Glutamic Acid; D003546:Cysteine Endopeptidases; C072775:HC-Pro protein, potyvirus; D008239:Lysine