Experimental autoimmune labyrinthitis: assessment of molecular size of autoantigens in fractions of inner ear proteins eluted on the Mini Whole Gel Eluter.
In order to determine the molecular size of the causative autoantigens of experimental autoimmune labyrinthitis, crude inner ear antigen was separated into 14 fractions and the constituent molecules were identified by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis using the Mini Whole Gel Eluter. The mean total protein recovery was approximately 66%. Sensitization with 55-65 kDa proteins induced the highest number of infiltrating cells among the fractions and sensitization with 38-45 kDa proteins induced the second highest number of inflammatory cells. These results suggest that 38-45 kDa and 55-65 kDa proteins are the causative autoantigens of experimental autoimmune labyrinthitis.
Tomiyama
Shunichi
S
Department of Otolaryngology, Nippon Medical School, Tama-Nagayama Hospital, Tokyo, Japan. tomii@nms.ac.jp
D000818:Animals; D001324:Autoantigens / Q000276:immunology* / Q000378:metabolism*; D002458:Cell Fractionation / Q000295:instrumentation; D003051:Cochlea / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D003520:Cyclophosphamide; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation; D007074:Immunoglobulin G / Q000276:immunology; D007166:Immunosuppressive Agents; D007762:Labyrinthitis / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000473:pathology; D011506:Proteins / Q000378:metabolism*
D001324:Autoantigens; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; D011506:Proteins; D003520:Cyclophosphamide