[Experimental study of anti-vascular endothelial growth factor hairpin ribozyme gene inhibiting growth of xenografted tumor of ovarian cancer cells].
OBJECTIVE
We propose to block the autocrine and (or) paracrine pathway of vascular endothelial growth factor (VEGF) in ovarian cancer by using anti-VEGF hairpin ribozyme gene to see whether the growth of tumor cells could be inhibited and to further exploit its mechanisms.


METHODS
Anti-VEGF hairpin ribozyme gene eukaryotic expression vector was introduced into ovarian cancer cells SKOV(3) by using lipofectin mediation and positive clones were screened by genticin (G418); ribozyme (RZ) expression was confirmed by RNA dot blot; the VEGF expression of SKOV(3) cells before or after transfection were detected by reverse transcription polymerase chain reaction (RT-PCR) method, transmission electron microscopy demonstrated the morphologic changes of ribozyme-generating SKOV(3) cells; the growth of transfected cells in nude mice were detected.


RESULTS
VEGF expression was decreased distinctly in SKOV(3) cells transfected by RZ (SKOV(3)-RZ) cells. Apoptosis cells were observed; the formation rate and growth speed of xenografted tumor slowed down.


CONCLUSIONS
Anti-VEGF hairpin ribozyme gene can inhibit the expression of VEGF mRNA and VEGF in SKOV(3) cells. The growth of xenografted tumor in nude mice was inhibited by reduced angiogenesis. This provides an experimental basis for curing human ovarian cancer with antiangiogenesis method.
Yan
Ruilan
R
Department of Gynecology and Obstetrics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Xin
Xiaoyan
X
Jin
Ming
M
Hui
Hongxiang
H
Wang
Jian
J
Wang
Detang
D
D000818:Animals; D016228:Endothelial Growth Factors / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000502:physiology; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000502:physiology; D008222:Lymphokines / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000473:pathology / Q000628:therapy*; D016337:RNA, Catalytic / Q000627:therapeutic use*; D014162:Transfection; D014183:Transplantation, Heterologous; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D016337:RNA, Catalytic; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors