Outer membrane protein A (OmpA): a new pathogen-associated molecular pattern that interacts with antigen presenting cells-impact on vaccine strategies.
Outer membrane protein A (OmpA) is a class of proteins highly conserved among the Enterobacteriaceae family and throughout evolution. We have observed that antigen presenting cells (APCs) recognize and are activated by the recombinant OmpA from Klebsiella pneumoniae (KpOmpA). KpOmpA triggers cytokine production by macrophages and dendritic cells (DC), induces DC maturation and signals via Toll-like receptor 2. KpOmpA also interacts with endocytic receptor(s) expressed on DC and macrophages. Tumor antigens coupled to KpOmpA are taken up by APCs and gain access to the MHC class I pathway, triggering the initiation of protective anti-tumor cytotoxic responses in the absence of CD4 T cell help and adjuvant. Thus, OmpA appears as a new type of pathogen-associated molecular pattern (PAMP) usable as a vector in anti-infectious and therapeutic anti-tumor vaccines to elicit CTLs.
Jeannin
Pascale
P
Centre d'Immunologie Pierre Fabre, 5, Avenue Napoléon III, F-74164 Saint-Julien en Genevois, France. pascale.jeannin@pierre-fabre.com
Magistrelli
Giovanni
G
Goetsch
Liliane
L
Haeuw
Jean-Francois
JF
Thieblemont
Nathalie
N
Bonnefoy
Jean-Yves
JY
Delneste
Yves
Y
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology* / Q000378:metabolism; D001428:Bacterial Vaccines; D003713:Dendritic Cells / Q000276:immunology; D006801:Humans; D008264:Macrophages / Q000276:immunology; D008562:Membrane Glycoproteins / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D015398:Signal Transduction; D051195:Toll-Like Receptor 2; D051193:Toll-Like Receptors
D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2; D051193:Toll-Like Receptors; C078167:OMPA outer membrane proteins