[Effects of newborn bull serum and vitamins on cryopreservation of mouse seminiferous epithelial cells].
OBJECTIVE
To investigate the effects of newborn bull serum(NBS), vitamin C and vitamin E on cryopreservation of mouse seminiferous epithelial cells.


METHODS
The seminiferous epithelial cells from 7-day-old mice were cryopreserved in different freezing solutions. The cell recoveries were examined by Trypan blue exclusive staining after thawing. The freezing solutions composed of DMEM, 10% dimethylsulphoxide(DMSO), and 0, 5%, 10%, or 20% NBS, respectively, or composed of DMEM, 10% DMSO, 10% NBS, and 150 micrograms/ml vitamin C or 50 micrograms/ml vitamin E, respectively.


RESULTS
The cell recoveries in freezing solution containing 0, 5%, 10%, or 20% NBS were 83.4%, 84.7%, 85.7% and 83.6%, respectively. There were no significant differences between them. The cell recoveries in freezing solution containing vitamin C or vitamin E were 88.0% and 82.9%, respectively. There was no significant differences compared with that in freezing solution containing 10% DMSO and 10% NBS.


CONCLUSIONS
NBS, vitamin C and vitamin E have no significant protecting effects on mouse seminiferous epithelial cells, and can not significantly improve the cell recoveries.
Li
Lian-Jun
LJ
Department of Preclinical Veterinary Medicine, Faculty of Animal Science and Technology, Quartermaster University of PLA, Changchun, Jilin 130062, China. llian63@sohu.com
Li
De-Xue
DX
Zhang
Xue-Ming
XM
Yue
Zhan-Peng
ZP
Wen
Xing-Hao
XH
Luo
Bing-Kun
BK
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002417:Cattle; D015925:Cryopreservation; D004847:Epithelial Cells / Q000502:physiology; D005312:Fetal Blood / Q000502:physiology*; D008297:Male; D051379:Mice; D012670:Seminiferous Epithelium / Q000166:cytology*; D014812:Vitamin K / Q000494:pharmacology*
D014812:Vitamin K; D001205:Ascorbic Acid