Induction of MC-1 immunoreactivity in axons after injection of the Fc fragment of human immunoglobulins in macaque monkeys.
Although previous studies have suggested an increased activation of humoral immunity in neurodegenerative diseases, it remains unclear whether this phenomenon is secondary to lesion formation or contributes directly to their development. Using stereotaxic injections in macaque monkey cerebral cortex, we studied the effects of human immunoglobulins on the neuronal cytoskeleton. Under these conditions, several MC-1-immunoreactive axons were observed in the vicinity of injection site. No MC-1 or TG-3 staining was detected in neuronal soma. Ultrastructurally, several axons in the same area displayed curly formations and accumulation of twisted tubules but not paired helical filaments. These data suggest that Fc fragment induce conformational changes of tau and subtle structural alterations in axons in this model. Immunocytochemical analyses in human autopsy materials revealed the presence of human Fc fragments as well as Fc receptors only in large pyramidal neurons known to be vulnerable in brain aging and Alzheimer's disease, further supporting a possible role of immunoglobulins in neurodegeneration.
Bouras
Constantin
C
Department of Psychiatry, HUG Belle-Idée, University of Geneva School of Medicine, Switzerland. Constantin.Bouras@medecine.unige.ch
Riederer
Beat M
BM
Hof
Patrick R
PR
Giannakopoulos
Panteleimon
P
D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000378:metabolism* / Q000648:ultrastructure; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000008:administration & dosage / Q000378:metabolism*; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000378:metabolism*; D007150:Immunohistochemistry; D007267:Injections; D008252:Macaca fascicularis; D008297:Male; D009474:Neurons / Q000378:metabolism; D011961:Receptors, Fc / Q000378:metabolism; D013238:Stereotaxic Techniques; D014018:Tissue Distribution
D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D011961:Receptors, Fc