Microbial cell-surface display.
Cell-surface display allows peptides and proteins to be displayed on the surface of microbial cells by fusing them with the anchoring motifs. The protein to be displayed - the passenger protein - can be fused to an anchoring motif - the carrier protein - by N-terminal fusion, C-terminal fusion or sandwich fusion. The characteristics of carrier protein, passenger protein and host cell, and fusion method all affect the efficiency of surface display of proteins. Microbial cell-surface display has many potential applications, including live vaccine development, peptide library screening, bioconversion using whole cell biocatalyst and bioadsorption.
Lee
Sang Yup
SY
Metabolic and Biomolecular Engineering National Research Laboratory, Daejeon, South Korea. leesy@mail.kaist.ac.kr
Choi
Jong Hyun
JH
Xu
Zhaohui
Z
D000327:Adsorption; D020816:Amino Acid Motifs; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D002462:Cell Membrane / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005821:Genetic Techniques; D005822:Genetic Vectors; D006090:Gram-Negative Bacteria / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D011993:Recombinant Fusion Proteins