title: Affinity labeling of the nuclear vitamin D receptor with nonsteroidal alkylating agents.
abstract: Synthesis of an affinity alkylating non steroidal mimic of 1alpha,25-dihydroxyvitamin D(3) and its radiolabeled counterpart is presented. We also report the affinity labeling of the VDR-ligand binding domain (VDR-LBD) with this analogue.
authors.affiliation: Bioorganic Chemistry and Structural Biology, Section in Endocrinology, Diabetes and Metabolism, Department of Medicine, Boston University School of Medicine, 85 East Newton Street, Boston, MA 02118, USA.
authors.forename: Ana
authors.initials: A
authors.lastname: Fernández-Gacio
authors.forename: Carlos
authors.initials: C
authors.lastname: Fernández-Marcos
authors.forename: Narasimha
authors.initials: N
authors.lastname: Swamy
authors.forename: Rahul
authors.initials: R
authors.lastname: Ray
mesh_terms: D000345:Affinity Labels; D000477:Alkylating Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D002117:Calcitriol / Q000737:chemistry; D004967:Estrogens / Q000737:chemistry / Q000494:pharmacology; D007202:Indicators and Reagents; D018716:Molecular Mimicry; D018167:Receptors, Calcitriol / Q000187:drug effects*; D018160:Receptors, Cytoplasmic and Nuclear / Q000187:drug effects*; D011960:Receptors, Estrogen / Q000187:drug effects
chemical_list: D000345:Affinity Labels; D000477:Alkylating Agents; D004967:Estrogens; D007202:Indicators and Reagents; D018167:Receptors, Calcitriol; D018160:Receptors, Cytoplasmic and Nuclear; D011960:Receptors, Estrogen; D002117:Calcitriol