Effect of irrigation solutions for arthroscopic surgery on intraarticular tissue: comparison in human meniscus-derived primary cell culture between lactate Ringer's solution and saline solution.
In order to determine whether there is a difference in effect on cell morphology and function between two common arthroscopic irrigation solutions, primary cultures of cells derived from the surgically excised human menisci were incubated for 3 or 6 h in lactated Ringer's solution, isotonic sodium chloride solution, or serum-free cell culture medium (negative-control condition). Cell integrity was blindly evaluated by three independent examiners scoring photomicrographs of the cell cultures on a battery of five-point scales for abnormality of cell shape, irregularity of cell membrane, change of cell size and cell density. Cell cultures were also quantitatively assayed by semi-quantitative reverse-transcription-polymerase-chain-reaction for mRNA of alpha1 (I) procollagen, alpha1 (II) procollagen, aggrecan and heat-shock protein 70 to assess functional consequences of exposure to the solutions. There was a statistically significant difference in cell integrity scores between either lactated Ringer's solution or serum-free cell-culture medium and isotonic sodium chloride solution with greater damage to cells displayed. Scores for lactated Ringer's solution did not differ from those for serum-free cell-culture medium. There were no significant differences in mRNA expression level among the treatment conditions. It was concluded that the lactated Ringer's solution better maintained human meniscus cell integrity than the isotonic saline.
Shinjo
Hirotaka
H
Department of Orthopaedic Surgery, Osaka University Medical School, 2-2 Yamada-oka, Suita City, Osaka 565-0871, Japan.
Nakata
Ken
K
Shino
Konsei
K
Hamada
Masayuki
M
Nakamura
Norimasa
N
Mae
Tatsuo
T
Miyama
Takahide
T
Horibe
Shuji
S
Yoshikawa
Hideki
H
Ochi
Takahiro
T
D000293:Adolescent; D000328:Adult; D053674:Aggrecans; D001182:Arthroscopy; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics; D024043:Collagen Type II / Q000235:genetics; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D016326:Extracellular Matrix Proteins; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D007552:Isotonic Solutions / Q000494:pharmacology*; D037181:Lectins, C-Type; D008297:Male; D008592:Menisci, Tibial / Q000166:cytology*; D008875:Middle Aged; D011509:Proteoglycans / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D000077325:Ringer's Lactate; D012965:Sodium Chloride / Q000494:pharmacology*; D007507:Therapeutic Irrigation; D014158:Transcription, Genetic / Q000187:drug effects
D053674:Aggrecans; D024042:Collagen Type I; D024043:Collagen Type II; D016895:Culture Media, Serum-Free; D016326:Extracellular Matrix Proteins; D018840:HSP70 Heat-Shock Proteins; D007552:Isotonic Solutions; D037181:Lectins, C-Type; D011509:Proteoglycans; D012333:RNA, Messenger; D000077325:Ringer's Lactate; D012965:Sodium Chloride