title: Atrial natriuretic peptide gene transfer by means of intranasal administration attenuates airway reactivity in a mouse model of allergic sensitization.
abstract: Atrial natriuretic peptide (ANP) is a bronchodilator; however, the short half-life of ANP in vivo limited its therapeutic utility to treat asthma. The efficacy of intranasally administered plasmid DNA-expressing ANP (pANP; amino acid 99-126; Acc. No. XM131840) on the prevention of allergen-induced airway hyperresponsiveness (AHR) was examined in this study by using a mouse model of asthma. Ovalbumin-sensitized mice were treated with pANP versus control plasmids, and AHR was monitored after ovalbumin challenge for 5 weeks on 10-day intervals starting 4 days after gene transfer. Mice administered pANP demonstrated significantly less AHR for 20 days after treatment. The results demonstrate that pANP gene transfer protects against AHR and might be useful in the treatment of asthma.
authors.affiliation: Division of Allergy and Immunology-Joy McCann Culverhouse Airway Disease Center, University of South Florida College of Medicine and James A. Haley Veteran's Hospital, Tampa 33612, USA.
authors.forename: Mukesh
authors.initials: M
authors.lastname: Kumar
authors.forename: Aruna K
authors.initials: AK
authors.lastname: Behera
authors.forename: Richard F
authors.initials: RF
authors.lastname: Lockey
authors.forename: David L
authors.initials: DL
authors.lastname: Vesely
authors.forename: Shyam S
authors.initials: SS
authors.lastname: Mohapatra
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D001249:Asthma / Q000628:therapy*; D009320:Atrial Natriuretic Factor / Q000235:genetics*; D016535:Bronchial Hyperreactivity / Q000517:prevention & control*; D004195:Disease Models, Animal; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D008168:Lung / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology
chemical_list: D009320:Atrial Natriuretic Factor; D010047:Ovalbumin