title: Recombinant GroES in combination with CpG oligodeoxynucleotides protects mice against Mycobacterium avium infection.
abstract: The groES gene of Mycobacterium avium strain 485 was cloned and expressed in Escherichia coli and the recombinant GroES protein was purified by affinity chromatography. The GroES preparation showed high purity by electrophoresis and immunoblotting. Immuno-electron microscopy showed that GroES was located both in the cytoplasm and on the surface of the mycobacterial cells and thus is readily available to interact with the host immune system. BALB/c mice were immunised intranasally with recombinant GroES, alone or in combination with a synthetic oligodeoxynucleotide containing unmethylated CpG motifs, and tested for protection against infection with M. avium. Neither GroES nor CpG alone provided any protection against subsequent challenge with M. avium, whereas a combination of the two significantly protected the lungs and spleen against colonisation by M. avium after intranasal challenge with a low dose of the organism. This indicates that intranasal administration of GroES and CpG oligodeoxynucleotides increases the resistance of BALB/c mice to M. avium infection.
authors.forename: Lanfranco
authors.initials: L
authors.lastname: Fattorini
authors.forename: Roberta
authors.initials: R
authors.lastname: Creti
authors.forename: Roberto
authors.initials: R
authors.lastname: Nisini
authors.forename: Roberta
authors.initials: R
authors.lastname: Pietrobono
authors.forename: Yuming
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Laboratory of Bacteriology and Medical Mycology and *Laboratory of Ultrastructures, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors.forename: Annarita
authors.initials: A
authors.lastname: Stringaro
authors.affiliation: Laboratory of Bacteriology and Medical Mycology and *Laboratory of Ultrastructures, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Arancia
authors.forename: Ottaviano
authors.initials: O
authors.lastname: Serlupi-Crescenzi
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Iona
authors.forename: Graziella
authors.initials: G
authors.lastname: Orefici
mesh_terms: D000276:Adjuvants, Immunologic; D000281:Administration, Intranasal; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018835:Chaperonin 10 / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D002846:Chromatography, Affinity; D004269:DNA, Bacterial / Q000737:chemistry; D004926:Escherichia coli; D006801:Humans; D008168:Lung / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009162:Mycobacterium avium / Q000235:genetics* / Q000276:immunology*; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000382:microbiology / Q000473:pathology; D014376:Tuberculosis / Q000276:immunology / Q000517:prevention & control*
chemical_list: D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D018835:Chaperonin 10; D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins