Background-free, fast protein staining in sodium dodecyl sulfate polyacrylamide gel using counterion dyes, zincon and ethyl violet.
A background-free, fast protein staining method in polyacrylamide gel electrophoresis using an acidic dye, zincon (ZC) and a basic dye, ethyl violet (EV) is described. It is based on the counterion dye staining technique that employs two oppositely charged dyes to form an ion-pair complex in staining solution. The selective binding of free dye molecules to proteins in acidic solution produces bluish violet-colored bands. It is a rapid and end-point staining procedure, involving only fixing and staining steps that are completed in 1-1.5 h. The detection limit of this method is 8-15 ng of protein that is comparable to the sensitivity of the colloidal Coomassie Brilliant Blue G (CBBG) stain. Due to its sensitivity and speed, this stain may be more practical than any other dye-based stains for routine laboratory purposes.
Choi
Jung-Kap
JK
College of Pharmacy, Chonnam National University, Kwangju, South Korea. jkchoi@chonnam.ac.kr
Tak
Keong-Hoon
KH
Jin
Li-Tai
LT
Hwang
Sun-Young
SY
Kwon
Tae-Ik
TI
Yoo
Gyurng-Soo
GS
D001391:Azo Compounds; D004396:Coloring Agents; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005562:Formazans; D007202:Indicators and Reagents; D011506:Proteins / Q000032:analysis*; D012394:Rosaniline Dyes; D012967:Sodium Dodecyl Sulfate
D001391:Azo Compounds; D004396:Coloring Agents; D005562:Formazans; D007202:Indicators and Reagents; D011506:Proteins; D012394:Rosaniline Dyes; D012967:Sodium Dodecyl Sulfate; C009809:2-carboxy-2'-hydroxy-5'-sulfoformazylbenzene; C034887:ethyl violet