Effects of Annona squamosa extract on early pregnancy in rats.
Annona squamosa Linn., family Annonaceae, is said to show varied medicinal effects, including insecticide, antiovulatory and abortifacient. The purpose of present study was to investigate if A. squamosa seed aqueous extract, in doses higher than that popularly used to provoke abortion, interferes with reproductive performance, and to correlate the ingestion of this extract with possible alterations in rat embryonic implantation. Doses of 300 mg/kg (Treated Group I, n = 17) and 600 mg/kg (Treated Group II, n = 12) body wt. were administered by gavage, during days 1 to 5 of pregnancy (preimplantation period). The control group (n = 13) received water in the same manner, during the same period for comparison with experimental groups. The animals were euthanized on day 10 of pregnancy. Treatment of dams during the preimplantation period showed no signs of toxicity, and no alteration in the corpora lutea, implantations and embryo in terms of development numbers. The percentage of preimplantation and postimplantation losses in treated groups I and II did not differ from those of control. Treatment with aqueous extract of A. squamosa seeds caused no morphological change in the endometrium. The absence of morphological alterations in uterine epithelial cells in treated groups I and II permitted a viable embryonic implantation, as verified by the number of embryos in development at day 10 of pregnancy. Thus, A. squamosa seed aqueous extract did not interfere with the reproductive performance of pregnant rats.
Damasceno
D C
DC
Experimental Laboratory of Obstetrics, Department of Gynecology and Obstetrics of School of Human Medicine of Botucatu-UNESP, São Paulo State, Brazil. decaito@bol.com.br
Volpato
G T
GT
Sartori
T C F
TC
Rodrigues
P F
PF
Perin
E A
EA
Calderon
I M P
IM
Rudge
M V C
MV
D000020:Abortifacient Agents, Nonsteroidal / Q000494:pharmacology; D000818:Animals; D031041:Annona; D001755:Blastocyst / Q000187:drug effects; D003338:Corpus Luteum / Q000187:drug effects; D005314:Embryonic and Fetal Development / Q000187:drug effects; D004717:Endometrium / Q000187:drug effects; D005260:Female; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry
D000020:Abortifacient Agents, Nonsteroidal; D010936:Plant Extracts