Effects of selected ginsenosides on phorbol ester-induced expression of cyclooxygenase-2 and activation of NF-kappaB and ERK1/2 in mouse skin.
Our previous studies have demonstrated that the methanol extract of heat-processed Panax ginseng C.A. Meyer exerts antioxidative, antiinflammatory, and anti-tumor-promoting effects. In the present study, we examined the antiinflammatory effects of several ginsenosides (Rb(1), Rc, Re, Rg(1), Rg(3)) derived from P. ginseng. Topical application of each of these ginsenosides significantly attenuated ear edema induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). These ginsenosides also suppressed expression of cyclooxygenase-2 (COX-2) and activation of NF-kappaB in the TPA-treated dorsal skin of mice. Of the ginsenosides tested, Rg(3) was found to be most effective in terms of inhibiting TPA-induced ear edema, COX-2 expression, and NF-kappaB activation.
Surh
Young-Joon
YJ
Laboratory of Biochemistry and Molecular Toxicology, College of Pharmacy, Seoul National University, Seoul 151-742, South Korea.
Lee
Ji-Yoon
JY
Choi
Kang-Ju
KJ
Ko
Sung-Ryong
SR
D000818:Animals; D051546:Cyclooxygenase 2; D004789:Enzyme Activation; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D036145:Ginsenosides / Q000494:pharmacology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000235:genetics*; D051379:Mice; D008813:Mice, Inbred ICR; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D009929:Organ Size / Q000187:drug effects; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics*; D012867:Skin / Q000187:drug effects / Q000201:enzymology*; D013329:Structure-Activity Relationship; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D036145:Ginsenosides; D007527:Isoenzymes; D016328:NF-kappa B; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D013755:Tetradecanoylphorbol Acetate