Histological examination of sublethal effects of diazinon on ovary of bluegill, Lepomis macrochirus.
The effects of the insecticide, diazinon (an organophosphorous compound), on the ovaries of bluegill (Lepomis macrohirus) were studied. Histological preparations of bluegill ovarian tissue was examined at 24, 48, 72 and 96 h and 1, 2 and 3 weeks following exposure to sublethal doses of diazinon (60 microg/l). The control contained primary follicles with an intact ovigerous lamellae and tunica albuginea. The control also revealed well developed stage IV oocytes with properly distributed provitelline nucleoli. After 24 h of exposure to diazinon, primary follicles began to show adhesion and cytoplasmic retraction in oocyte II occured. Cytoplasmic degeneration and additional adhesion and more retraction were visible at 48 h in oocyte II; 72 h brought forth adhesion, retraction and cytoplamic expulsion from oocyte IV. The number of atretic oocytes increased. Damages to the oocyte IV started to occur after 72 h of exposure. Cytoplasmic retraction and clumping was more visible at 96 h in oocyte IV. Partial destruction of the ovigerous lamellae and vitellogenic membrane occurred after 1 week. Two weeks continued to reveal destruction of follicles. Severe damage of the ovigerous lamellae, increased intrafollicular spaces, vacuolated cytoplasm, extrusion of karyoplasm and necrosis in the cytoplasm were most evident following 3 weeks of exposure. The ovarian wall became frayed and broken. Additionally, a marked increase of atretic follicles, shrinkage, and embedded nucleoli into the surrounding cytoplasm in oocyte II, III and IV were observed at week 3. This study revealed that oocytes at their different stages of maturation get affected differently at various exposure. Based on observations of the ovarian tissue compositional and structural changes following given exposure times, it becomes evident that consistent sublethal doses of diazinon can and will alter microscopic anatomy of the fish ovary.
Dutta
Hiran M
HM
Department of Biological Sciences, Kent State University, OH 44242, USA. h.dutta@kent.edu
Maxwell
Laura B
LB
D000818:Animals; D003976:Diazinon / Q000506:poisoning*; D004781:Environmental Exposure; D005260:Female; D005399:Fishes; D007306:Insecticides / Q000506:poisoning*; D009865:Oocytes / Q000187:drug effects / Q000473:pathology; D006080:Ovarian Follicle / Q000187:drug effects / Q000473:pathology; D010053:Ovary / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors
D007306:Insecticides; D003976:Diazinon