Huperzine B protects rat pheochromocytoma cells against oxygen-glucose deprivation-induced injury.
OBJECTIVE
To test the ability of huperzine B (HupB) to alleviate injury from oxygen-glucose deprivation (OGD) in the rat pheochromocytoma line PC12 cells.


METHODS
After OGD for 3 h and reoxygenation for 24 h, neuronal morphology was observed by phase-contrast microscopy; cell survival was quantified by the reduction of MTT; malondialdehyde (MDA) was determined by the thiobarbituric acid; superoxide dismutase (SOD) was assayed by a modification of the xanthine/xanthine oxidase; and lactate (LA) was measured according to Marbach and Weil.


RESULTS
OGD for 3 h and reoxygenation for 24 h triggered death in nearly 70 % of cells, along with major changes in morphology and biochemistry including elevated level of MDA, SOD activity, and LA content. Cells pretreated with HupB 1-100 micromol/L for 2 h showed significantly improved survival and reduced biochemical and morphologic signs of toxicity.


CONCLUSIONS
HupB protected PC12 cells against OGD-induced injury, most likely by alleviating disturbances of oxidative and energy metabolism.
Wang
Zhi-Fei
ZF
State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Zhou
Jin
J
Tang
Xi-Can
XC
D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects; D005947:Glucose / Q000172:deficiency*; D032501:Huperzia / Q000737:chemistry*; D019344:Lactic Acid / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D016716:PC12 Cells; D010673:Pheochromocytoma / Q000473:pathology; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
D000470:Alkaloids; D018696:Neuroprotective Agents; C053016:huperzine B; D019344:Lactic Acid; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005947:Glucose