Directional restriction site-free insertion of PCR products into vectors.
Chen
Guo Jun
GJ
F. Hoffmann La-Roche, Basel, Switzerland.
D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases / Q000235:genetics; D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific; D005822:Genetic Vectors; D007202:Indicators and Reagents; D016254:Mutagenesis, Insertional / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping
D017931:DNA Primers; D007202:Indicators and Reagents; D002265:Carboxylic Ester Hydrolases; C019674:aminoacyl-tRNA hydrolase; C020102:endodeoxyribonuclease DpnI; D015252:Deoxyribonucleases, Type II Site-Specific