[The distribution of androgen receptor and its coexistence with gonadotropin releasing hormone receptor in rat submaxillary].
OBJECTIVE
To study the coexistence of androgen receptor(AR) and GnRH receptor(GnRHR), and further identify that submaxillary is a target organ of androgen and GnRH in SD Rat.


METHODS
Sequential deparaffinized sections of SD rat submaxillary were immunostained with SABC method. The first antibodies were rabbit anti-rat GnRH idiotypic antibodies and mouse anti-rat androgen receptor antibodies.


RESULTS
AR immunoreactive cells were found in glandular epithelial cells of serous acinus and epithelial cells in all gland ducts. While the distribution of GnRHR coincides with that of AR. The immunoreactive substances were distributed in cytoplasm of all positive cells with negative nuclei.


CONCLUSIONS
The results showed that AR existed in submaxillary and was widely distributed in glandular epithelial cells with distributive pattern similar to those of GnRHR. It suggests that submaxillary is a target organ of androgen, responsible for modulating biological function of submaxillary.
Yao
Bing
B
Laboratory of Reproduction & Genetics, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China.
Fan
Jun
J
Wang
Yong-Mei
YM
Cui
Ying-Xia
YX
Huang
Yu-Feng
YF
D000818:Animals; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011944:Receptors, Androgen / Q000378:metabolism*; D011966:Receptors, LHRH / Q000378:metabolism*; D013363:Submandibular Gland / Q000378:metabolism*
D011944:Receptors, Androgen; D011966:Receptors, LHRH