Microbial susceptibility to calcium hydroxide pastes and their vehicles.
The aim of this study was to investigate the susceptibility of some microorganisms commonly isolated from root canals to calcium hydroxide in combination with several vehicles by the agar diffusion method. Stainless-steel cylinders were placed on each inoculated agar medium. The test medications and their controls were placed inside the cylinders. The zones of growth inhibition were measured and recorded after the incubation period for each plate, and the results were analyzed statistically. Enterococcus faecalis was most resistant, whereas the anaerobic Porphyromonas endodontalis was more susceptible to all medications, followed by P. gingivalis and Prevotella intermedial intermedia. Ca(OH)2 + CMCP + glycerin showed significantly larger mean zones of inhibition when compared with the other medications. We conclude that anaerobic Gram-negative bacteria are more susceptible to calcium hydroxide pastes than facultative Gram-positive microorganisms.
Gomes
Brenda Paula Figueiredo de Almeida
BP
Dental School of Piracicaba, State University of Campinas, SP, Brazil.
Ferraz
Caio Cezar Randi
CC
Garrido
Fabio Devora
FD
Rosalen
Pedro Luiz
PL
Zaia
Alexandre Augusto
AA
Teixeira
Fabricio Batista
FB
de Souza-Filho
Francisco José
FJ
D000190:Actinomyces / Q000187:drug effects; D000362:Agar; D000704:Analysis of Variance; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002164:Camphor / Q000494:pharmacology; D002733:Chlorophenols / Q000494:pharmacology; D015169:Colony Count, Microbial; D003470:Culture Media; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004338:Drug Combinations; D024881:Drug Resistance, Bacterial; D013293:Enterococcus faecalis / Q000187:drug effects; D005079:Excipients; D005990:Glycerol / Q000494:pharmacology; D006089:Gram-Negative Anaerobic Bacteria / Q000187:drug effects; D006801:Humans; D009824:Ointments; D014677:Pharmaceutical Vehicles; D011092:Polyethylene Glycols / Q000494:pharmacology; D017861:Porphyromonas / Q000187:drug effects; D016966:Porphyromonas gingivalis / Q000187:drug effects; D018720:Prevotella / Q000187:drug effects; D018713:Prevotella intermedia / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D013291:Streptococcus / Q000187:drug effects
D002733:Chlorophenols; D003470:Culture Media; D004338:Drug Combinations; D005079:Excipients; D009824:Ointments; D014677:Pharmaceutical Vehicles; D012387:Root Canal Filling Materials; D011092:Polyethylene Glycols; D002164:Camphor; C029804:camphorated parachlorophenol; D000362:Agar; D005990:Glycerol; D002126:Calcium Hydroxide