[Analysis of proteins with altered expression in human esophageal squamous cell carcinomas].
OBJECTIVE
Esophageal squamous cell carcinoma is characterized by high incidence and high mortality. This study was designed to identify the proteins dysregulated in ECSS by.


METHODS
Microdissection of routinely unstained frozen sections was used to procure cancer cells from seven esophageal squamous cell carcinomas, and esophageal epithelial cells from normal tissues adjacent to the tumors. By two-dimensional electrophoresis, protein profiles of normal and cancerous tissues were obtained. Selected proteins with altered expression in tumors were identified through MALDI-TOF-MS.


RESULTS
Compared the protein profiles of tumors with those of normal epithelia, 11 proteins were found as dysregulated in tumors. Among them, HSP27 was observed up-expressed and three isoforms of ANNEXIN I were seen down-expressed in cancerous tissues.


CONCLUSIONS
Two-dimensional electrophoresis coupled with mass spectrometry can contribute to rapidly screen dysregulated proteins in human maligancies. Altered expressions of HSP27 and ANNEXIN I were the frequent events in Chinese esophageal squamous cell carcinomas.
Xia
Shu-hua
SH
National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, P. R. China.
Hu
Hai
H
Hu
Li-ping
LP
Xu
Xin
X
Cai
Yan
Y
Han
Ya-ling
YL
Chen
Bao-sheng
BS
Wei
Fang
F
Ying
Wan-tao
WT
Qian
Xiao-hong
XH
Cai
You-yu
YY
Shen
Yan
Y
Wu
Min
M
Wang
Ming-rong
MR
D002294:Carcinoma, Squamous Cell / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D004938:Esophageal Neoplasms / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D009363:Neoplasm Proteins / Q000032:analysis*
D009363:Neoplasm Proteins