LOX-1 pathway affects the extent of myocardial ischemia-reperfusion injury.
Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) was originally identified as a receptor for oxidized low-density lipoprotein predominantly expressed in endothelial cells. LOX-1 expression can be induced in cardiomyocytes and that activation of LOX-1 is involved in apoptosis. To investigate possible roles of LOX-1 in myocardial ischemia-reperfusion injury, rats were subjected to coronary artery ligation for 1h followed by reperfusion for 2h. Immunohistochemistry revealed that expression of LOX-1 in cardiac myocytes was induced following ischemia-reperfusion but not ischemia alone. Administration of anti-LOX-1 monoclonal antibody resulted in a nearly 50% reduction in myocardial infarction size compared with that of normal IgG or saline (P<0.05). These findings suggest that activation of the LOX-1 pathway is involved in determining the extent of myocardial ischemia-reperfusion injury and that inhibition of the LOX-1 pathway may provide a novel strategy for treatment of acute myocardial infarction in humans.
Kataoka
Kazuaki
K
Department of Cardiovascular Medicine, Graduate School of Medicine Kyoto University, Kyoto, Japan.
Hasegawa
Koji
K
Sawamura
Tatsuya
T
Fujita
Masatoshi
M
Yanazume
Tetsuhiko
T
Iwai-Kanai
Eri
E
Kawamura
Teruhisa
T
Hirai
Taku
T
Kita
Toru
T
Nohara
Ryuji
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017209:Apoptosis; D002452:Cell Count; D002478:Cells, Cultured; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008297:Male; D017202:Myocardial Ischemia / Q000473:pathology / Q000503:physiopathology; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D011973:Receptors, LDL / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051131:Receptors, Oxidized LDL; D015427:Reperfusion Injury / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control*; D051127:Scavenger Receptors, Class E; D016896:Treatment Outcome
D000911:Antibodies, Monoclonal; C495136:OLR1 protein, rat; D011973:Receptors, LDL; D051131:Receptors, Oxidized LDL; D051127:Scavenger Receptors, Class E