title: Application of micellar electrokinetic chromatography to the determination of sultamicillin in oral pharmaceutical preparations.
abstract: A micellar electrokinetic capillary electrophoretic method for determination of sultamicillin in Unasyn oral preparations--tablets and suspension--was evaluated. Phosphate-borate buffer at pH 7.0 containing 1.0% sodium dodecylsulfate was used as a mobile phase. The elaborated method ensures separation of sultamicillin from p-toluenesulfonic acid and the impurities, ampicillin, sulbactam and penicillamine. The method was validated for specificity, reproducibility, precision, accuracy and assay linearity (in a concentration range of sultamicillin of 0.05-1.5 mg/ml). Statistical analysis by Student's t-test showed no significant differences between the results obtained by micellar electrokinetic chromatography and HPLC, t(calculated) 0.519 for suspension assays and 0.284 for tablets assays were smaller then t(tabulated).
authors.affiliation: Antibiotics and Microbiology Department, Drug Institute, 30/34 Chełmska St., 00-725 Warsaw, Poland. tyski@il.waw.pl
authors.forename: Genowefa
authors.initials: G
authors.lastname: Pajchel
authors.forename: Stefan
authors.initials: S
authors.lastname: Tyski
mesh_terms: D000284:Administration, Oral; D000667:Ampicillin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004359:Drug Therapy, Combination / Q000008:administration & dosage / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013407:Sulbactam / Q000032:analysis*
chemical_list: C035444:sultamicillin; D000667:Ampicillin; D013407:Sulbactam