[Basic fibroblast growth factor inhibits promoter activities of human alpha 1(I) procollagen gene induced by transforming growth factor-beta 1].
OBJECTIVE
To investigate the effects of basic fibroblast growth factor (bFGF) on the promoter activities of human alpha 1(I) procollagen gene and the interaction between bFGF and transforming growth factor-beta 1 (TGF-beta 1).


METHODS
Fibroblasts of the hypertrophic scar and normal skin from a 3-year-old patient were primarily cultured and subcultured in vitro. Both of the fibroblasts were transient transfected with phCOL 2.5, containing -2.5 kb of 5'f lank sequence of human alpha 1(I) procollagen gene and CAT reporter gene by FuGENE transfection reagent; and treated thereafter by 16 ng/ml bFGF, 2 ng/ml TGF-beta 1 and 16 ng/ml bFGF + 2 ng/ml TGF beta 1 for 24 hours. The relative CAT expression values were determined by CAT-ELISA.


RESULTS
TGF-beta 1 strongly induced the CAT expression level, however, bFGF not only inhibited the basal CAT expression but also reduced the CAT expression up-regulated by TGF-beta 1 in normal skin and hypertrophic scar fibroblasts (P < 0.05).


CONCLUSIONS
bFGF can reduce the promoter activities of human alpha 1(I) procollagen gene and antagonize the role of TGF-beta 1 in up-regulating the promoter activities of human alpha 1(I) procollagen gene in normal skin and hyertrophic scar fibroblasts.
Wan
Wei-dong
WD
Zhongda Hospital, Southeast University, Nanjing Jiangsu, P. R. China 210009.
Yang
Song-lin
SL
Gao
Chun-fang
CF
D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D002921:Cicatrix / Q000473:pathology / Q000517:prevention & control; D024042:Collagen Type I / Q000235:genetics*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors*
D024042:Collagen Type I; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D015500:Chloramphenicol O-Acetyltransferase