title: Effects of 14 days of head-down tilt bed rest on cutaneous vasoconstrictor responses in humans.
abstract: This study tested the hypothesis that head-down tilt bed rest (HDBR) reduces adrenergic and nonadrenergic cutaneous vasoconstrictor responsiveness. Additionally, an exercise countermeasure group was included to identify whether exercise during bed rest might counteract any vasoconstrictor deficits that arose during HDBR. Twenty-two subjects underwent 14 days of strict 6 degrees HDBR. Eight of these 22 subjects did not exercise during HDBR, while 14 of these subjects exercised on a supine cycle ergometer for 90 min a day at 75% of pre-bed rest heart rate maximum. To assess alpha-adrenergic vasoconstrictor responsiveness, intradermal microdialysis was used to locally administer norepinephrine (NE), while forearm skin blood flow (SkBF; laser-Doppler flowmetry) was monitored over microdialysis membranes. Nonlinear regression modeling was used to identify the effective drug concentration that caused 50% of the cutaneous vasoconstrictor response (EC(50)) and minimum values from the SkBF-NE dose-response curves. In addition, the effects of HDBR on nonadrenergic cutaneous vasoconstriction were assessed via the venoarteriolar response of the forearm and leg. HDBR did not alter EC(50) or the magnitude of cutaneous vasoconstriction to exogenous NE administration regardless of whether the subjects exercised during HDBR. Moreover, HDBR did not alter the forearm venoarteriolar response in either the control or exercise groups during HDBR. However, HDBR significantly reduced the magnitude of cutaneous vasoconstriction due to the venoarteriolar response in the leg, and this response was similarly reduced in the exercise group. These data suggest that HDBR does not alter cutaneous vasoconstrictor responses to exogenous NE administration, whereas cutaneous vasoconstriction of the leg due to the venoarteriolar response is reduced after HDBR. It remains unclear whether attenuated venoarteriolar responses in the lower limbs contribute to reduced orthostatic tolerance after bed rest and spaceflight.
authors.affiliation: Institute for Exercise and Environmental Medicine, Presbyterian Hospital of Dallas, Dallas, TX 75231, USA.
authors.forename: Thad E
authors.initials: TE
authors.lastname: Wilson
authors.forename: Manabu
authors.initials: M
authors.lastname: Shibasaki
authors.forename: Jian
authors.initials: J
authors.lastname: Cui
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Levine
authors.forename: Craig G
authors.initials: CG
authors.lastname: Crandall
mesh_terms: D000328:Adult; D001160:Arterioles / Q000502:physiology; D001510:Bed Rest; D001808:Blood Vessels / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005542:Forearm; D018475:Head-Down Tilt; D006801:Humans; D007866:Leg; D008297:Male; D009638:Norepinephrine / Q000008:administration & dosage; D012018:Reflex / Q000502:physiology; D012867:Skin / Q000098:blood supply*; D013997:Time Factors; D014661:Vasoconstriction / Q000502:physiology*; D014662:Vasoconstrictor Agents / Q000008:administration & dosage; D014680:Veins / Q000502:physiology
chemical_list: D014662:Vasoconstrictor Agents; D009638:Norepinephrine