Synthesis and high content cell-based profiling of simplified analogues of the microtubule stabilizer (+)-discodermolide.
(+)-Discodermolide, a C24:4, trihydroxylated, octamethyl, carbamate-bearing fatty acid lactone originally isolated from a Caribbean sponge, has proven to be the most potent of the microtubule-stabilizing agents. Recent studies suggest that it or its analogues may have advantages over other classes of microtubule-stabilizing agents. (+)-Discodermolide's complex molecular architecture has made structure-activity relationship analysis in this class of compounds a formidable task. The goal of this study was to prepare simplified analogues of (+)-discodermolide and to analyze their biological activities to expand structure-activity relationships. A small library of analogues was prepared wherein the (+)-discodermolide methyl groups at C-14 and C-16 and the C-7 hydroxyl were removed, and the lactone was replaced by simple esters. The library components were analyzed for microtubule-stabilizing actions in vitro, antiproliferative activity against a small panel of human carcinoma cells, and cell signaling, microtubule architecture and mitotic spindle alterations by a multiparameter fluorescence cell-based screening technique. The results show that even drastic structural simplification can lead to analogues with actions related to microtubule targeting and signal transduction, but that these subtle effects were illuminated only through the high information content cell-based screen.
Minguez
Jose M
JM
Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA.
Giuliano
Kenneth A
KA
Balachandran
Raghavan
R
Madiraju
Charitha
C
Curran
Dennis P
DP
Day
Billy W
BW
D000473:Alkanes; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001665:Binding Sites; D002219:Carbamates; D002244:Carbon / Q000737:chemistry; D002277:Carcinoma / Q000473:pathology*; D002455:Cell Division; D004305:Dose-Response Relationship, Drug; D015723:Gene Library; D006367:HeLa Cells; D006801:Humans; D007783:Lactones / Q000494:pharmacology*; D008870:Microtubules / Q000187:drug effects* / Q000378:metabolism; D008956:Models, Chemical; D017239:Paclitaxel / Q000494:pharmacology; D010766:Phosphorylation; D011753:Pyrones; D015398:Signal Transduction; D008941:Spindle Apparatus; D014404:Tubulin / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000473:Alkanes; D000970:Antineoplastic Agents; D002219:Carbamates; D007783:Lactones; D011753:Pyrones; D014404:Tubulin; D002244:Carbon; C071229:discodermolide; D017239:Paclitaxel