[Characterization of thermophilic spore-forming bacteria from a geothermal spring in Lithuania based on 16S rDNA and 16S-23S rDNA intergenic spacers analyses].
Forty-two strains of gram-positive, aerobic, heterotrophic, obligately thermophilic, spore-forming bacteria were isolated from a geothermal site near the Baltic Sea in Lithuania. All of the strains were able to hydrolyze collagen and/or casein. Since characteristics of proteolytic activity are correlated with taxonomic positions of bacteria, the strains were grouped on the basis of molecular biological analyses. On the basis of RFLP patterns of 16S rDNA and 16S-23S rDNA ITS-PCR analysis, the strains were subdivided into nine groups.
Kuĭsene
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Department of Plant Physiology and Microbiology, Faculty of Natural Sciences, Vilnius University, Chiurlionio 21/27, Vilnius, 2009 Lithuania. nomeda.kuisiene@gf.vu.lt
Emantene
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R
Valiunas
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Chitavichus
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D
D002364:Caseins / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis*; D021903:DNA, Ribosomal Spacer / Q000032:analysis*; D016936:Gram-Positive Endospore-Forming Bacteria / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D008097:Lithuania; D010447:Peptide Hydrolases; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D014871:Water Microbiology
D002364:Caseins; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D003094:Collagen; D010447:Peptide Hydrolases