Binding of the human homolog of the Drosophila discs large tumor suppressor protein to the mitochondrial ribosomal protein MRP-S34.
The human homolog of the Drosophila discs large tumor suppressor protein (hDLG) functions as a scaffolding protein that facilitates the transmission of diverse downstream signals. Here we show that hDLG interacts through its PDZ domains with the carboxy-terminal S/TXV motif of the mitochondrial ribosomal protein S-34 (MRP-S34). Our results suggest that hDLG interacts with MRP-S34 prior to entry of MRP-S34 into the mitochondria and may be involved in the trafficking of MRP-S34.
Ogawa
Fumiaki
F
Laboratory of Molecular and Genetic Information, Institute for Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
Adachi
Shungo
S
Kohu
Kazuyoshi
K
Shige
Kenjiro
K
Akiyama
Tetsu
T
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D000074288:Discs Large Homolog 1 Protein; D004330:Drosophila; D029721:Drosophila Proteins; D006801:Humans; D015151:Immunoblotting; D019476:Insect Proteins / Q000235:genetics*; D008565:Membrane Proteins; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011485:Protein Binding / Q000502:physiology; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D048868:Adaptor Proteins, Signal Transducing; C513314:DLG1 protein, human; D000074288:Discs Large Homolog 1 Protein; D029721:Drosophila Proteins; D019476:Insect Proteins; C471039:MRPS34 protein, human; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D011506:Proteins; D012269:Ribosomal Proteins; D025521:Tumor Suppressor Proteins; C070350:dlg1 protein, Drosophila