Reactive arthritis and serum levels of mannose binding lectin -- lack of association.
The purpose was to evaluate the possible association of serum mannose binding lectin (s-MBL) levels on type of triggering microbe, duration of diarrhoea, incidence and course of reactive arthritis (ReA) caused by Salmonella, Yersinia and Campylobacter. Sixty patients with ReA of 1-228 months duration, 173 patients with ReA or uncomplicated enterocolitis caused by Campylobacter, 226 sera from patients with elevated antibody levels against Salmonella, Yersinia or Campylobacter, and 114 blood donors were tested for s-MBL using ELISA technique, both direct mannan binding assay and sandwich ELISA. s-MBL was compared with C-reactive protein (CRP) levels and with the ability of activating complement C4. Among the 114 donors 9% had s-MBL <50 microg/l, 16% had from 50-500 microg/l and 75% had >500 microg/l. The distribution of s-MBL levels in the three-patient groups did not differ significantly from the controls. There were no indications that low s-MBL was associated with prolonged duration of arthritis, diarrhoea or individual bacterial infections. The two MBL assays were comparable with respect to serum concentrations, indicating that the actual circulating MBL was also functionally active. s-MBL exhibited acute phase reactant behaviour and correlated to CRP level, but only in patients with s-MBL concentrations exceeding 1000 microg/l. MBL in 10 randomly selected ReA sera were tested for the ability to activate complement C4. The results did not differ from those of donor controls. This study demonstrates that the distributions of s-MBL levels in serum among patients with ReA are not different from donor controls. The course, outcome or triggering bacteria are not associated with a particular level of s-MBL.
Locht
H
H
Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark. hlo@ssi.dk
Christiansen
M
M
Laursen
I
I
D000293:Adolescent; D000328:Adult; D000368:Aged; D016918:Arthritis, Reactive / Q000097:blood* / Q000382:microbiology; D002097:C-Reactive Protein / Q000032:analysis; D002169:Campylobacter Infections / Q000097:blood*; D003181:Complement C4 / Q000032:analysis; D003967:Diarrhea / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood*; D008875:Middle Aged; D000091402:Prohibitins; D012480:Salmonella Infections / Q000097:blood*; D015009:Yersinia Infections / Q000097:blood*
D003181:Complement C4; D037601:Mannose-Binding Lectin; C000717331:PHB2 protein, human; D000091402:Prohibitins; D002097:C-Reactive Protein