title: Electrochemical properties of Nile Blue covalently immobilized on self-assembled thiol-monolayer modified gold electrodes.
abstract: A monolayer of Nile Blue (NB) has been covalently immobilized on the self-assembled thiol-monolayer modified gold electrode. Cyclic voltammograms indicated a stable and reverse redox process of NB bonded on the electrode surface. The mechanisms of redox process coupling with proton transfer were proposed. The NB-modified electrode showed excellent electrocatalytic activity toward Nicotinamide adenine dinucleotide (NADH) oxidation and horseradish peroxidase (HRP) reduction. A hydrogen peroxide biosensor based on NB as a mediator has been demonstrated.
authors.affiliation: Department of Chemistry, Wuhan University, Wuhan 430072, China.
authors.forename: Hui-Hong
authors.initials: HH
authors.lastname: Liu
authors.forename: Ji-Lin
authors.initials: JL
authors.lastname: Lu
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Dai-Wen
authors.initials: DW
authors.lastname: Pang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold; D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D009243:NAD / Q000378:metabolism; D010078:Oxazines / Q000737:chemistry*; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds
chemical_list: D010078:Oxazines; D013438:Sulfhydryl Compounds; D009243:NAD; C008619:Nile Blue; D006046:Gold; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase