title: Megakaryoblastic leukemia cell line MOLM-16 derived from minimally differentiated acute leukemia with myeloid/NK precursor phenotype.
abstract: The megakaryoblastic leukemia cell line MOLM-16 was established at relapse from the peripheral blood of a 77-year-old Japanese woman with minimally differentiated acute myeloid leukemia (AML-M0). Immunophenotyping of the fresh leukemic cells revealed a myeloid/NK precursor phenotype being positive for CD7, CD13, CD33, CD34, and CD56. In addition, megakaryocyte-associated antigens CD41 and CD61 were found to be positive. The established cell line designated MOLM-16 was proliferatively responsive to the treatment with various cytokines including EPO, GM-CSF, IL-3, PIXY-321, and TPO. MOLM-16 revealed characteristics of the megakaryocytic lineage in terms of immunophenotyping being positive for CD9, CD31, CD36, CD41, CD61, CD62P, CD63, CD110, CD151, thrombospondin, von Willebrand factor (vWf), and fibrinogen. Electron microscopic analysis showed positivity for ultrastructural platelet peroxidase in the nuclear envelope. The karyotype analysis of MOLM-16 revealed various numerical and structural abnormalities including t(6;8)(q21;q24.3), t(9;18)(q13;q21) and marker chromosomes. The extensive immunological, cytogenetic and functional characterization of MOLM-16 suggests that this cell line may represent a scientifically significant in vitro model which could facilitate the evaluation of megakaryocytic differentiation.
authors.affiliation: Fujisaki Cell Center, Hayashibara Biochemical Labs Inc., Fujisaki, Okayama 702-8006, Japan. yomatsuo@hayashibara.co.jp
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Matsuo
authors.forename: Hans G
authors.initials: HG
authors.lastname: Drexler
authors.forename: Kinuyo
authors.initials: K
authors.lastname: Kaneda
authors.forename: Kensuke
authors.initials: K
authors.lastname: Kojima
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ohtsuki
authors.forename: Masamichi
authors.initials: M
authors.lastname: Hara
authors.forename: Masaki
authors.initials: M
authors.lastname: Yasukawa
authors.forename: Mitsune
authors.initials: M
authors.lastname: Tanimoto
authors.forename: Kunzo
authors.initials: K
authors.lastname: Orita
mesh_terms: D000368:Aged; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D019070:Cell Lineage; D002887:Chromosomes, Human, Pair 18; D002896:Chromosomes, Human, Pair 6; D002898:Chromosomes, Human, Pair 8; D002899:Chromosomes, Human, Pair 9; D020732:Cytogenetic Analysis; D016207:Cytokines / Q000494:pharmacology; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000276:immunology / Q000473:pathology; D007947:Leukemia, Megakaryoblastic, Acute / Q000235:genetics / Q000473:pathology*; D008855:Microscopy, Electron, Scanning; D022423:Myeloid Cells / Q000276:immunology / Q000473:pathology; D012008:Recurrence; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured / Q000166:cytology*
chemical_list: D016207:Cytokines