Novel surface modification of polymer-based separation media controlling separation selectivity, retentivity and generation of electroosmotic flow.
Uniformly sized packing materials based on synthetic polymer particles for high-performance liquid chromatography (HPLC) and capillary electrochromatography (CEC) have been prepared from polymerization mixtures containing methacrylic acid (MAA) as a functional monomer and by using a novel surface modification method. This "dispersion method" affords effectively modified separation media. Both the amount of MAA utilized in the preparation and reaction time affect the selectivity of chromatographic separation in both the HPLC and the CEC mode and electroosmotic flow. This detailed study revealed that the dispersion method effectively modified internal surface of macroporous separation media and, based on the amount of MAA introduced, exclusion mechanism for the separation of certain solutes could be observed.
Hosoya
Ken
K
Department of Polymer Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. kenpc@ipc.kit.ac.jp
Kubo
Takuya
T
Takahashi
Katsuo
K
Ikegami
Tohru
T
Tanaka
Nobuo
N
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation*; D009995:Osmosis; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013499:Surface Properties
D011108:Polymers