Immunohistochemical localization of transforming growth factor alpha in regenerating rat liver.
Immunohistochemical localization of TGF-alpha and cell proliferation kinetics during liver regeneration after two-thirds partial hepatectomy (PH) were investigated. Twenty-four to 72 hr after PH, appreciable increase in the number of TGF-alpha-positive hepatocytes was observed in zones 1 and 2. At the peak at 36 hr, almost all positive cells were stained in their nuclei. Considerable increase in the BrdU labeling index was observed 24-36 hr after PH with a peak at 24 hr in zones 1 and 2. These results indicated an association between TGF-alpha expression and hepatocyte regeneration. It is suggested that immunohistochemical localization of TGF-alpha may be a useful marker of cell proliferation activity in rat liver.
Okada
Miyoko
M
Toxicology Laboratory, Pharmaceuticals Research Center, Mitsubishi Pharma Corporation, Kisarazu, Chiba, Japan.
Sakairi
Tetsuya
T
Kusakabe
Manami
M
Goto
Kazuhiro
K
Tsuchiya
Takayuki
T
Sugimoto
Jiro
J
Sano
Fumiko
F
Mutai
Mamoru
M
Morohashi
Tetsuo
T
Kobayashi
Kiyoshi
K
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002455:Cell Division; D006498:Hepatectomy; D022781:Hepatocytes / Q000737:chemistry; D007150:Immunohistochemistry; D008099:Liver / Q000737:chemistry* / Q000166:cytology; D008115:Liver Regeneration; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D013997:Time Factors; D016211:Transforming Growth Factor alpha / Q000032:analysis*
D015415:Biomarkers; D016211:Transforming Growth Factor alpha