Galectin 1 inhibits incorporation of vitronectin and chondroitin sulfate B into the extracellular matrix of human vascular smooth muscle cells.
Galectin-1, a beta-galactoside-binding dimeric lectin, interacts with the extracellular matrix (ECM) of smooth muscle cells (SMCs) and with particular ECM proteins. Enrichment of the ECM with galectin-1 affects adhesion and proliferation of cultured SMCs. Here we investigated whether galectin-1 (1) interacts with glycosaminoglycan (GAG) chains, (2) cross-links between ligands and facilitates the incorporation of GAGs, vitronectin and plasma fibronectin in the ECM of vascular SMCs. A recombinant galectin-1 fusion protein GalH, used in this study, formed dimers and interacted with ECM proteins. GAG chains inhibited these interactions. Among the studied GAG chains, only chondroitin sulfate B interacted with GalH in beta-galactoside-dependent manner. GalH did not bridge between ECM proteins on solid phase and [125I]-labelled ECM proteins or GAGs in solution. The ECM incorporated less vitronectin in the presence of soluble GalH. GalH-enriched ECM incorporated less vitronectin and chondroitin sulfate B. The ECM partially depleted of endogenous galectins incorporated more chondroitin sulfate B compared to untreated ECM. These results suggest that galectin-1 is likely to be involved in the ECM assembly affecting incorporation of some ECM components important for SMC behaviour.
Moiseeva
Elena P
EP
Division of Cardiology, Department of Medicine, University of Leicester, Clinical Sciences Wing, Glenfield General Hospital, Leicester LE3 9QP, UK. em9@le.ac.uk
Williams
Bryan
B
Samani
Nilesh J
NJ
D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry; D003871:Dermatan Sulfate / Q000378:metabolism*; D019281:Dimerization; D005109:Extracellular Matrix / Q000378:metabolism*; D037483:Galectin 1 / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D019096:Vitronectin / Q000378:metabolism*
D003432:Cross-Linking Reagents; D037483:Galectin 1; C445499:LGALS1 protein, human; D011993:Recombinant Fusion Proteins; D019096:Vitronectin; D003871:Dermatan Sulfate