Cycloheximide prevents inhibition of expression of immediate early gene c-fos in paraventricular nuclei of rat hypothalamus produced by delta sleep-inducing peptide.
We studied expression of the immediate early gene c-fos in hypothalamic paraventricular nuclei in rats with different prognostic resistance to emotional stress receiving delta-sleep-inducing peptide after intracerebroventricular administration of cycloheximide. Delta-sleep-inducing peptide inhibited expression of the c-fos gene in rats receiving intracerebroventricular injection of physiological saline. Pretreatment with the protein synthesis blocker cycloheximide abolished changes produced by delta-sleep-inducing peptide.
Umriukhin
P E
PE
P. K. Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia.
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology*; D003701:Delta Sleep-Inducing Peptide / Q000494:pharmacology*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects* / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
D011500:Protein Synthesis Inhibitors; D016760:Proto-Oncogene Proteins c-fos; D003701:Delta Sleep-Inducing Peptide; D003513:Cycloheximide