title: Nutritionally induced obesity is attenuated in transgenic mice overexpressing plasminogen activator inhibitor-1.
abstract: OBJECTIVE
  The objective of this study was to investigate the role of plasminogen activator inhibitor-1 (PAI-1) in adipose tissue development in vivo.
  
  
  RESULTS
  Transgenic (Tg) mice overexpressing murine PAI-1 under control of the adipocyte promoter aP2 and wild-type (WT) controls were kept on standard food (SFD) or on high-fat diet (HFD) for 15 weeks. The body weight and the weight of the isolated subcutaneous and gonadal fat deposits of the Tg mice kept on the HFD were significantly lower than those of the WT mice. The number of adipocytes in the adipose tissue was similar for Tg and WT mice on the HFD, but adipocyte hypotrophy and a significantly lower ratio of stroma cells/adipocytes were observed in the Tg mice. A significant negative correlation (P<0.01) was observed between expression of preadipocyte factor-1, which blocks adipocyte differentiation, and adipose tissue weight. Fasting insulin and total cholesterol levels on the HFD were lower in Tg than in WT mice.
  
  
  CONCLUSIONS
  High circulating PAI-1 levels attenuate nutritionally induced obesity. This may be related to modifications in adipose tissue cellularity affecting weight and plasma metabolic parameters.
authors.affiliation: Center for Molecular and Vascular Biology, University of Leuven, Belgium. roger.lijnen@med.kuleuven.ac.be
authors.forename: H Roger
authors.initials: HR
authors.lastname: Lijnen
authors.forename: Erik
authors.initials: E
authors.lastname: Maquoi
authors.forename: Pierre
authors.initials: P
authors.lastname: Morange
authors.forename: Gabor
authors.initials: G
authors.lastname: Voros
authors.forename: Berthe
authors.initials: B
authors.lastname: Van Hoef
authors.forename: Francis
authors.initials: F
authors.lastname: Kopp
authors.forename: Désiré
authors.initials: D
authors.lastname: Collen
authors.forename: Irène
authors.initials: I
authors.lastname: Juhan-Vague
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Alessi
mesh_terms: D000273:Adipose Tissue / Q000254:growth & development / Q000473:pathology / Q000502:physiology; D000818:Animals; D001835:Body Weight / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism*; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D009765:Obesity / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis* / Q000097:blood / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D002352:Carrier Proteins; D004041:Dietary Fats; C082667:Fabp5 protein, mouse; C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D017395:Plasminogen Activator Inhibitor 1