Effect of bone-like layer growth from culture medium on adherence of osteoblast-like cells.
The electrical polarization of ceramic HAp had an effect on the acceleration of bone restoration. Cell behavior in the bone-like growth layer was investigated. The deposits on the ceramic HAp was grown and formed layers by soaking in alpha-minimum essential medium supplemented with 10% fetal bovine serum (alpha-MEM supplemented with 10% FBS). The shapes of the adhering cells on the grown layer gradually changed from spindle to flat with growth of the layer. On the totally grown layer that was grown on the ceramic HAp by soaking in alpha-MEM supplemented with 10% FBS for 7 days, all the adhering cells were flat and the surface was filled with the grown cells. From these results, it was revealed that the grown layer on the ceramic HAp is one of the activation factors of cell growth. Consequently, cell growth was reinforced by acceleration of the layer growth on the negatively charged surface of the polarized ceramic HAp.
Kizuki
Takashi
T
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kandasurugadai, Chiyoda, Tokyo 1010062, Japan. takashi@kizuki.net
Ohgaki
Masataka
M
Katsura
Mihoko
M
Nakamura
Satoshi
S
Hashimoto
Kazuaki
K
Toda
Yoshitomo
Y
Udagawa
Shigekazu
S
Yamashita
Kimihiro
K
D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000502:physiology*; D002455:Cell Division; D016764:Cell Polarity; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D017886:Durapatite; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*
D001672:Biocompatible Materials; D003470:Culture Media; D017886:Durapatite