Characterization of a novel human breast cancer associated gene (BCA3) encoding an alternatively spliced proline-rich protein.
As part of an integrated study of breast cancer gene expression, partial cDNAs were cloned from normal and tumor breast cells by subtractive-hybridization and differential display cloning. The DNA sequence for one of these breast cancer associated genes was used to construct the larger 1319 bp BCA3 cDNA sequence using ESTs without assigned names or functions. High-level BCA3 mRNA expression was found in breast and prostate tumor cell lines whereas normal breast and prostate tissues have low-level expression. Further analysis revealed possible functional domains and alternative splicing of BCA3 that we confirmed by RT-PCR analysis. Immunohistochemistry revealed that the protein is expressed in breast tumor cells in vivo, and not in surrounding stromal tissue.
Kitching
Richard
R
Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Canada.
Li
Haoxia
H
Wong
Michael J
MJ
Kanaganayakam
Shankary
S
Kahn
Harriette
H
Seth
Arun
A
D048868:Adaptor Proteins, Signal Transducing; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000235:genetics*; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D009687:Nuclear Proteins; D009928:Organ Specificity; D011392:Proline / Q000378:metabolism; D011506:Proteins / Q000235:genetics*; D016415:Sequence Alignment
C471621:AKIP1 protein, human; D048868:Adaptor Proteins, Signal Transducing; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D011506:Proteins; D011392:Proline