[Study on the xenoantigens of pancreatic tissues of Chinese Neijiang pig].
The expression and distribution of xenoantigens in pancreatic tissue of Neijiang pig were investigated with immunohistochemistry technique. BSIB4, normal human serum and anti-human A, B, H monoclonal antibodies were used as the primary antibody to examine the expression of xenoantigens in the pancreatic tissues from 10 Neijiang pigs. The results demonstrate that compared with the negative results of human blood B and H type antigens, the alpha-Gal antigen was highly expressed on the pancreatic vascular endothelial cells and the duct epithelium. The human blood A type antigen was highly expressed on some islet cells, some exocrine secretory cells and all the duct epithelium. Since there are pre-exited xenoantibodies against pig pancreas cells in human serum, cross-matching test and graft pre-treatment are needed before clinical transplantation of pancreas. The expression of xenoantigens in Neijiang pig is different from other pig strains reported by others, which suggests that looking for a pig strain that contains less xenoantigens is possible.
Bu
H
H
Lab. of Transplantation Immunology, First Affiliated Hospital, WCUMS, Chengdu 610041.
Chen
Y
Y
Li
Y
Y
Cheng
J
J
Li
T
T
D000017:ABO Blood-Group System / Q000276:immunology; D000818:Animals; D015478:Antigens, Heterophile / Q000032:analysis*; D006801:Humans; D008297:Male; D010179:Pancreas / Q000276:immunology*; D013552:Swine; D014312:Trisaccharides / Q000032:analysis*
D000017:ABO Blood-Group System; D015478:Antigens, Heterophile; D014312:Trisaccharides; C076569:alpha-galactosyl epitope