[Developmental toxicity of Al(NO3)3 on rat embryos].
The developmental toxicity and its mechanism of Al(NO3)3 on embryos in SD rats were studied. On the 9.5th day of gestation, the embryos were incubated in a whole-embryo culture system with Al(NO3)3 at concentrations of Al3+ from 0.6 to 9.0 micrograms/ml in culture media for 48 hrs. Viable embryos were evaluated by Brown's morphological scoring system, and the diameter of yolk sac, crown-rump, head length and the dry weight of embryos were measured. There was a dose-dependent relations of decreasing embryo development with increasing concentrations of Al3+. Yolk sac diameter, head length, dry weight and heart, forelimb as well as neural tube scores decreased significantly at 1.2 micrograms/ml (P < 0.05). When embryos were exposed to Al3+ at > or = 3.0 micrograms/ml, the embryonic development and morphological differentiation were obviously inhibited(P < 0.05); meanwhile, the incidence of dysmorphogenesis significantly increased, including neural tube defects and dorsiflexion teratogenesis. The results suggested that aluminum might be a developmental toxicant and dysmorphogenesis agent.
Zhang
B
B
Department of Public Health, Lanzhou Medical College, Lanzhou 730000, China.
Gao
X
X
Wu
D
D
D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D046508:Culture Techniques; D004622:Embryo, Mammalian; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D008297:Male; D009566:Nitrates / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013723:Teratogens / Q000633:toxicity*
D017607:Aluminum Compounds; D009566:Nitrates; D013723:Teratogens; C050609:aluminum nitrate