title: The solution structure of the loop E region of the 5S rRNA from spinach chloroplasts.
abstract: A structure has been obtained for the loop E region of the 5S rRNA from Spinacia oleracia chloroplast ribosomes using residual dipolar coupling data as well as NOE, J coupling and chemical shift information. Even though the loop E sequence of this chloroplast 5S rRNA differs from that of Escherichia coli loop E at approximately 40% of its positions, its conformation is remarkably similar to that of E.coli loop E. Consistent with this conclusion, ribosomal protein L25 from E.coli, which binds to the loop E region of both intact E.coli 5S rRNA and to oligonucleotides containing that sequence, also binds to the chloroplast-derived oligonucleotide discussed here.
authors.affiliation: Department of Chemistry, Yale University, New Haven, CT 06520-8107, USA.
authors.forename: Pramodh
authors.initials: P
authors.lastname: Vallurupalli
authors.forename: Peter B
authors.initials: PB
authors.lastname: Moore
mesh_terms: D001482:Base Composition; D002736:Chloroplasts / Q000737:chemistry* / Q000235:genetics; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009690:Nucleic Acid Conformation; D011485:Protein Binding / Q000235:genetics; D014176:Protein Biosynthesis; D011522:Protons; D012329:RNA, Bacterial / Q000737:chemistry; D018749:RNA, Plant / Q000737:chemistry*; D012341:RNA, Ribosomal, 5S / Q000737:chemistry*; D012269:Ribosomal Proteins / Q000737:chemistry / Q000378:metabolism; D012996:Solutions; D018724:Spinacia oleracea / Q000737:chemistry* / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D011522:Protons; D012329:RNA, Bacterial; D018749:RNA, Plant; D012341:RNA, Ribosomal, 5S; D012269:Ribosomal Proteins; D012996:Solutions; C031127:ribosomal protein L25