[Study on HIV DNA of epidemic HIV-1 strains].
OBJECTIVE
To study the characteristics of variation of HIV DNA in prevalent HIV-1 strains.


METHODS
HIV DNA, extracted from peripheral blood mononuclear cell(PBMC) in 63 patients infected with HIV, was investigated by reverse-transcript PCR (RT-PCR), Long-Distance PCR(LD-PCR), molecular cloning hybridization and sequencing, respectively.


RESULTS
The subtypes were identified in 57 samples. Subtype B was identified in 43 cases, subtype C in 5 and subtype E in 9. It was found that both full-length and defective HIV DNA in 31 out of 57 cases, defective HIV DNA in 19 cases and full-length HIV DNA in 7 cases. The deleted regions of genome were from 71 to 7,759 nucleotides.


CONCLUSIONS
The variation of gene segments of HIV DNA plays an important role in the infection and epidemic of HIV.
Xu
X
X
Department of Infectious Diseases, First Clinical Hospital, Beijing Medical University, Beijing 100034, China.
Xing
H
H
Chen
L
L
D000328:Adult; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D015658:HIV Infections / Q000235:genetics / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004279:DNA, Viral