Cloning and characterization of a novel UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase, pp-GalNAc-T14.
A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) gene family was cloned and designated pp-GalNAc-T14. This type II membrane protein contains all motifs that are conserved in the pp-GalNAc-T family proteins and forms a subfamily with pp-GalNAc-T2 on the phylogenetic tree. Quantitative real time PCR analysis revealed significantly high expression of the pp-GalNAc-T14 transcript in kidney, although the transcripts were ubiquitously expressed in all tissues examined. Furthermore, the recombinant pp-GalNAc-T14 transferred GalNAc to a panel of mucin-derived peptide substrates such as Muc2, Muc5AC, Muc7, and Muc13 (-58). Our results provide evidence that pp-GalNAc-T14 is a new member of the pp-GalNAc-T family and suggest that pp-GalNAc-T14 may be involved in the O-glycosylation in kidney.
Wang
Han
H
Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Open Space Laboratory Central-2, 1-1-1 Umezono, Tsukuba, Ibaraki-ken 305-8568, Japan.
Tachibana
Kouichi
K
Zhang
Yan
Y
Iwasaki
Hiroko
H
Kameyama
Akihiko
A
Cheng
Lamei
L
Guo
Jian ming
Jm
Hiruma
Toru
T
Togayachi
Akira
A
Kudo
Takashi
T
Kikuchi
Norihiro
N
Narimatsu
Hisashi
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D005091:Exons; D015698:Genomic Library; D006031:Glycosylation; D006801:Humans; D007438:Introns; D007668:Kidney / Q000201:enzymology; D008565:Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D017350:N-Acetylgalactosaminyltransferases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D009928:Organ Specificity; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity; D000097763:Polypeptide N-acetylgalactosaminyltransferase
D008565:Membrane Proteins; D017350:N-Acetylgalactosaminyltransferases; D000097763:Polypeptide N-acetylgalactosaminyltransferase; C471038:UDP-N-acetyl-D-galactosamine polypeptide N-acetylgalactosaminyltransferase 14, human