Taenia saginata derived synthetic peptides with potential for the diagnosis of bovine cysticercosis.
Immunity in Taeniids is predominantly antibody mediated and thus many serological immuno-determinants will have potential in both protection and diagnosis. The antigenicity of six peptides derived from four potentially protective molecules cloned from a Taenia saginata oncospheres cDNA library have been evaluated as targets for the specific diagnosis of bovine cysticercosis. The six peptides consist of: two peptides (HP6-2 and HP6-3) derived from the sequence of the 18 kDa surface/secreted oncospheral adhesion antigen identified by McAb-HP6, two peptides (Ts45W-1 and Ts45W-5) derived from the sequence of the T. saginata homologue of the T. ovis 45W protective gene family, one peptide (TS45S-10) derived from a T. saginata sequence with significant similarity to the T. ovis 45S protective antigen, and one peptide (TEG-1) derived from the sequence of the T. saginata homologue of Echinococcus spp. main surface protein. Longitudinal studies indicate that T. saginata infected cattle respond to all six peptides by 3-4 weeks post-infection and that the antibody levels remain high for at least 12 weeks post-infection. As protection against Taeniid parasites is predominantly antibody mediated, some of these six peptides may be of value as immuno-prophylactic tools and hence also in assays to determine resistance to infection with the parasite. For diagnosis, on the other hand, only three peptides (HP6-2, TEG-1 and Ts45S-10) performed with the necessary sensitivity and specificity to determine exposure to infection with T. saginata, and now merit an exhaustive evaluation prior to employment as routine diagnostic tools.
Ferrer
E
E
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey GU24 ONF, UK.
Benitez
L
L
Foster-Cuevas
M
M
Bryce
D
D
Wamae
L W
LW
Onyango-Abuje
J A
JA
Garate
T
T
Harrison
L J S
LJ
Parkhouse
R M E
RM
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D003429:Cross Reactions; D003551:Cysticercosis / Q000175:diagnosis* / Q000276:immunology / Q000662:veterinary*; D005260:Female; D007106:Immune Sera / Q000276:immunology; D008297:Male; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D041202:Taenia saginata / Q000276:immunology*; D013997:Time Factors
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007106:Immune Sera; D010455:Peptides