Induction of a 72-kDa heat-shock protein in cultured rat gastric mucosal cells and rat gastric mucosa by zinc L-carnosine.
An antiulcer drug, zinc L-carnosine (polaprezinc), provides gastric mucosal protection against various irritants. In this study, we evaluated the effects of zinc L-carnosine on expression of 72-kDa heat shock protein (HSP72, stress inducible HSP70), which is known as an endogenous cytoprotectant in a wide variety of cells, including rat gastric mucosa in vitro and in vivo. Expression of HSP72 after exposure to zinc L-carnosine, zinc sulfate, or L-carnosine (1-300 microM) in rat gastric mucosal cells (RGM1) and intragastric administration of zinc L-carnosine, zinc sulfate (30 or 100 mg/kg) and L-carnosine (76 mg/kg) was investigated by western blotting and densitometric analysis. Exposure to zinc L-carnosine and zinc sulfate increased the expression of HSP72 significantly in RGM1 cells. Intragastric administration of zinc L-carnosine and zinc sulfate showed significant increment in HSP72 in rat gastric mucosa also in vivo. The ability to induce HSP72 is significantly higher in zinc L-carnosine compared with zinc sulfate based on molecular concentration in vivo. However, L-carnosine did not increase the expression of HSP72 in vitro and in vivo. Zinc derivatives, especially zinc L-carnosine, could be a strong HSP72 (chaperon) inducer, which has been known to enhance mucosal protective ability.
Odashima
Masaru
M
First Department of Internal Medicine, Akita University School of Medicine, Akita City, Akita, Japan.
Otaka
Michiro
M
Jin
Mario
M
Konishi
Noriaki
N
Sato
Toshihiro
T
Kato
Sayuri
S
Matsuhashi
Tamotsu
T
Nakamura
Chieko
C
Watanabe
Sumio
S
D000818:Animals; D002336:Carnosine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002478:Cells, Cultured; D005752:Gastric Mucins / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008297:Male; D009942:Organometallic Compounds / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015032:Zinc; D017967:Zinc Compounds; D019287:Zinc Sulfate / Q000494:pharmacology
D005752:Gastric Mucins; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D009942:Organometallic Compounds; D017967:Zinc Compounds; C061957:polaprezinc; D019287:Zinc Sulfate; D002336:Carnosine; D015032:Zinc