[Studies on single cell cloning of Ginkgo biloba L].
OBJECTIVE
To investigate the relations of the productivity stability of to the homogenization of Ginkgo biloba cells by single cell clones.


METHODS
Using plant single cell plate culture technique.


RESULTS
Adding 500 ml.L-1L-glutamine in the medium increased greatly the cell plating efficiency (PE), and within the range 0.5-5.0 x 10(4) Cell.ml-1 of the initial cell density, the PE increased with the increment of the cell density. Of the 48 cell clones obtained, the GKB content in G-22 clone reached 0.099%; and most of the clones remain stable in growth and GKB production during subcultures.


CONCLUSIONS
During the cell and tissue culture of G. biloba, the application of plant single cell cloning technique helps to a certain extent solve the problem of productivity instability of ginkgolide.
Dai
J G
JG
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Zhu
W H
WH
Wu
Y Q
YQ
Hu
Q
Q
Zhang
D Y
DY
D002460:Cell Line; D002999:Clone Cells; D004224:Diterpenes; D020441:Ginkgo biloba / Q000166:cytology* / Q000378:metabolism; D046934:Ginkgolides; D007783:Lactones / Q000378:metabolism; D010946:Plants, Medicinal / Q000166:cytology* / Q000378:metabolism
D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; C045856:ginkgolide B