Mass spectrometric detection of nodularin and desmethylnodularin in mussels and flounders.
Samples of mussels and flounders from the Baltic Sea were analysed for nodularin content on two different LC-MS instruments (triple quadrupole and ion trap). The triple quadrupole instrument was well suited for the quantitative analysis. The limit of detection in the selected ion recording mode was 5 pg and in the multiple reactant monitoring mode 500 pg on column for extracts of Nodularia spumigena. The fragmentation patterns of nodularin-R and desmethylnodularin-R were recorded and shown to be similar to those of microcystins. LC-MS proved to be an excellent tool for the analyses of cyanobacterial hepatotoxins in complex matrices.
Karlsson
Krister
K
Department of Biochemistry and Pharmacy, Abo Akademi University, 20521, Turku, Finland.
Sipiä
Vesa
V
Kankaanpää
Harri
H
Meriluoto
Jussi
J
D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D005432:Flounder; D013058:Mass Spectrometry / Q000379:methods*; D010456:Peptides, Cyclic / Q000032:analysis*
D010456:Peptides, Cyclic; C475949:desmethylnodularin; C063998:nodularin