title: Nocardiopsis metallicus sp. nov., a metal-leaching actinomycete isolated from an alkaline slag dump.
abstract: A taxonomic study was carried out on a metal-mobilizing, alkaliphilic bacterium from an alkaline slag dump, strain KBS6(T). The strain produced substrate and aerial mycelia. Growth occurred in the pH range 7.0-10.5, with an optimum at pH 8.5. A salt concentration of up to 10% was tolerated, and various organic substrates were used for growth. The results of a 16S rDNA sequence comparison revealed that strain KBS6(T) belongs to the genus Nocardiopsis. DNA-DNA hybridization with the two closest relatives, Nocardiopsis exhalans and Nocardiopsis prasina, gave similarity values of 18.2 and 44.1%, respectively, which indicated that strain KBS6(T) represents a novel species of the genus Nocardiopsis. This is consistent with the morphological, physiological and chemotaxonomic data. Because of the ability of this micro-organism to solubilize metals, the name Nocardiopsis metallicus sp. nov. is proposed for strain KBS6(T) (= DSM 44598(T) = NRRL B-24159(T)), this being the type strain.
authors.forename: Axel
authors.initials: A
authors.lastname: Schippers
authors.forename: Klaus
authors.initials: K
authors.lastname: Bosecker
authors.forename: Sabine
authors.initials: S
authors.lastname: Willscher
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.forename: Reiner M
authors.initials: RM
authors.lastname: Kroppenstedt
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D008670:Metals; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S