Lincomycin resistance mutations in two regions immediately downstream of the -10 region of lmr promoter cause overexpression of a putative multidrug efflux pump in Bacillus subtilis mutants.
We isolated 19 lincomycin-resistant Bacillus subtilis mutants by expressing lmrB encoding a putative multidrug efflux protein. Eighteen of the mutants altered at two regions (-3 to -1 and +15) immediately downstream of the -10 region of the lmr promoter increased lmr transcription in vivo and in vitro.
Kumano
Miyuki
M
Institute of Biological Sciences, University of Tsukuba, Tsukuba-shi, Ibaraki 305-8572, Japan.
Fujita
Masaya
M
Nakamura
Kouji
K
Murata
Makiko
M
Ohki
Reiko
R
Yamane
Kunio
K
D001412:Bacillus subtilis / Q000235:genetics* / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D008034:Lincomycin / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009154:Mutation
D008034:Lincomycin