[Kinetic parameters of hydrolysis of CpA and UpA sequences in an oligoribonucleotide by compounds functionally mimicking ribonuclease A].
Kinetic parameters of cleavage of CpA and UpA sequences in an oligoribonucleotide under the action of artificial ribonuclease ABL3C1 were measured. The compounds were built of RNA-binding domain B, catalytic fragment C, linker L3 comprising 3 methylene groups, and aliphatic fragment A. The rate of cleavage of phosphodiester bonds in CpA sequence within decaribonucleotide UUCAUGUAAA was shown to be 3.4 +/- 0.2 times higher than in UpA sequence. The rate of cleavage of phosphodiester bonds were found to depend on substrate length: a thousandfold increase in cleavage rate constant was observed for CpA sequence in decaribonucleotide as compared with diribonucleotide monophosphate CpA. A slight decrease in the cleavage rates was observed for the reactions proceeding in different buffers at pH 7.0: imidazole > HEPES > phosphate > cacodylate. At the same time, the ratio of cleavage rates for CpA and UpA sequences remained constant.
Beloglazova
N G
NG
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
Mironova
N L
NL
Konevets
D A
DA
Petiuk
V A
VA
Sil'nikov
V N
VN
Vlasov
V V
VV
Zenkova
M A
MA
D001372:Aza Compounds / Q000737:chemistry*; D001665:Binding Sites; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D002384:Catalysis; D015226:Dinucleoside Phosphates / Q000737:chemistry; D006868:Hydrolysis; D007093:Imidazoles / Q000737:chemistry*; D007700:Kinetics; D018716:Molecular Mimicry; D009843:Oligoribonucleotides / Q000737:chemistry*; D012313:RNA / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000737:chemistry; D012260:Ribonucleases / Q000737:chemistry*; D013329:Structure-Activity Relationship
D001372:Aza Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D015226:Dinucleoside Phosphates; D007093:Imidazoles; D009843:Oligoribonucleotides; C009627:uridylyl-(2'-5')-adenosine; C013907:cytidylyl adenosine; D012313:RNA; D012260:Ribonucleases; D012259:Ribonuclease, Pancreatic