Activated SRC protein tyrosine kinase is overexpressed in late-stage human ovarian cancers.
OBJECTIVE
The objective of this study was to determine if the Src tyrosine kinase is overexpressed and activated in late-stage human ovarian cancers.


METHODS
Western analysis and immune complex kinase assays were performed on a panel of human ovarian cancer cell lines and normal ovarian epithelial cell cultures, and immunohistochemical analysis for Src and activated Src were performed on a panel of late-stage human ovarian tumors.


CONCLUSIONS
Src is overexpressed and activated in a majority of late-stage ovarian tumors as well as in a panel of cultured malignant human ovarian epithelium grown in vitro, but not in normal ovarian epithelium (NOE) or immortalized NOE. Src overexpression was found to be frequently, but not always, associated with HER-2/neu overexpression, but no statistical association between Src and Her-2/neu overexpression could be demonstrated.
Wiener
Jon R
JR
Department of Molecular Therapeutics, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA. Jon.R.Wiener@uth.tmc.edu
Windham
T Christopher
TC
Estrella
Veronica C
VC
Parikh
Nila U
NU
Thall
Peter F
PF
Deavers
Michael T
MT
Bast
Robert C
RC
Mills
Gordon B
GB
Gallick
Gary E
GE
D004789:Enzyme Activation; D005260:Female; D019073:HT29 Cells / Q000201:enzymology; D006801:Humans; D007150:Immunohistochemistry; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000201:enzymology* / Q000473:pathology; D051575:Proto-Oncogene Proteins c-yes; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis; D014407:Tumor Cells, Cultured; D019061:src-Family Kinases / Q000096:biosynthesis / Q000378:metabolism*
D051575:Proto-Oncogene Proteins c-yes; D018719:Erb-b2 Receptor Tyrosine Kinases; D019061:src-Family Kinases; C489834:YES1 protein, human