Fibroblast and epidermal growth factors modulate proliferation and neural cell adhesion molecule expression in epithelial cells derived from the adult mouse tongue.
Lingual epithelial cells, including those of the taste buds, are regularly replaced by proliferative stem cells. We found that integrin beta(1), a keratinocyte stem cell marker, was expressed at the basal layer and taste buds of adult mouse tongue epithelium. We purified and cultured integrin beta(1)-positive cells (termed KT-1 cells), whose growth was stimulated by epidermal growth factor (EGF) and basic fibroblast growth factor (FGF-2). FGF-2 stimulation induced translocation of the FGF type I receptor (FGFR1) into nuclei, suggesting that the growth-stimulating effect of FGF-2 was mediated through FGFR1. EGF and FGF-2 also regulated cell surface expression of the neural cell adhesion molecule (N-CAM) in KT-1 cells. Anti-N-CAM antibody immunoreactivity was restricted to the gustatory epithelium and the nerves in the tongue epithelium, giving rise to the possibility that KT-1 may contain gustatory epithelial cells. KT-1 cells may thus be useful for analyzing the factors that regulate the growth and differentiation of lingual and gustatory epithelial cells in vitro.
Ookura
Tetsuya
T
National Food Research Institute, 2-1-12 Kan-nondai, Tsukuba, Ibaraki 305-8642, Japan. ookura@nfri.affrc.go.jp
Kawamoto
Keiko
K
Tsuzaki
Hidetoshi
H
Mikami
Yoshinori
Y
Ito
Yumiko
Y
Oh
Seong-Lee
SL
Hino
Akihiro
A
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D018929:Cell Culture Techniques; D002455:Cell Division / Q000502:physiology; D004847:Epithelial Cells / Q000378:metabolism / Q000502:physiology*; D066246:ErbB Receptors / Q000502:physiology*; D005786:Gene Expression Regulation; D019012:Integrin beta1 / Q000502:physiology; D051379:Mice; D017468:Receptors, Fibroblast Growth Factor / Q000502:physiology*; D013650:Taste Buds / Q000737:chemistry / Q000196:embryology / Q000254:growth & development; D014059:Tongue / Q000166:cytology* / Q000294:innervation
D000943:Antigens, Differentiation; D015816:Cell Adhesion Molecules, Neuronal; D019012:Integrin beta1; D017468:Receptors, Fibroblast Growth Factor; D066246:ErbB Receptors