title: Simian virus 40 sequences in human lymphoblastoid B-cell lines.
abstract: Human Epstein-Barr virus-immortalized lymphoblastoid B-cell lines tested positive by PCR for simian virus 40 (SV40) DNA (22 of 42 cell lines, 52.3%). B lymphocytes or tissues from which B-cell lines derived were also SV40 positive. In situ hybridization showed that SV40 DNA was present in the nucleus of a small fraction (1/250) of cells. SV40 T-antigen mRNA was detected by reverse transcription-PCR. Lymphoblastoid B-cell lines (n = 4) infected with SV40 remained SV40 positive for 4 to 6 months. SV40-positive B-cell lines were more tumorigenic in SCID mice than were SV40-negative cell lines (4 of 5 [80%] SV40-positive cell lines versus 2 of 4 [50%] SV40-negative cell lines). These results suggest that SV40 may play a role in the early phases of human lymphomagenesis.
authors.affiliation: Divisions of Experimental Oncology, Centro di Riferimento Oncologico, IRCCS, 33081 Aviano (Pordenone), Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Dolcetti
authors.forename: Fernanda
authors.initials: F
authors.lastname: Martini
authors.forename: Michele
authors.initials: M
authors.lastname: Quaia
authors.forename: Annunziata
authors.initials: A
authors.lastname: Gloghini
authors.forename: Beatrice
authors.initials: B
authors.lastname: Vignocchi
authors.forename: Roberta
authors.initials: R
authors.lastname: Cariati
authors.forename: Marcella
authors.initials: M
authors.lastname: Martinelli
authors.forename: Antonino
authors.initials: A
authors.lastname: Carbone
authors.forename: Mauro
authors.initials: M
authors.lastname: Boiocchi
authors.forename: Mauro
authors.initials: M
authors.lastname: Tognon
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D001402:B-Lymphocytes / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013539:Simian virus 40 / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D012333:RNA, Messenger