title: [Prokaryotic expression of endostatin and preparation of polyclonal antibody].
abstract: OBJECTIVE
  The aim of this study was to express and purify human endostatin and to prepare polyclonal antibody of mouse anti-human endostatin.
  
  
  METHODS
  The cDNA of endostatin was amplified by PCR, then recombined into prokaryotic expression vector and transformed into Escherichia coli BL21 for expression; the mice were immunized with purified products.
  
  
  RESULTS
  Prokaryotic expression vector pQE-30 of human endostatin was successfully constructed; the expression product was gained after pQE-30 was transferred into BL21. After purified by Ni affinity chromatography, the product was identified to be a single component by SDS-PAGE. Western blot analysis showed that high titer mouse anti-human endostatin polyclonal antibody was successfully prepared.
  
  
  CONCLUSIONS
  Highly purified expression product and prepared polyclonal antibody provide the necessary material for further study.
authors.affiliation: Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, P. R. China. zym6677@sina.com.cn
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Zhang
authors.forename: Ruo-ning
authors.initials: RN
authors.lastname: Wang
authors.forename: Yan-li
authors.initials: YL
authors.lastname: Li
authors.forename: Dong-min
authors.initials: DM
authors.lastname: Gu
authors.forename: Zhi-xiang
authors.initials: ZX
authors.lastname: Cheng
authors.forename: Jun
authors.initials: J
authors.lastname: Xiong
authors.forename: Wei
authors.initials: W
authors.lastname: De
authors.forename: Bing-ying
authors.initials: BY
authors.lastname: Chen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D002846:Chromatography, Affinity; D003094:Collagen / Q000235:genetics* / Q000276:immunology; D043169:Endostatins; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D051379:Mice; D010446:Peptide Fragments / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D043169:Endostatins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D003094:Collagen