The promoter of the classical MHC class I locus in rainbow trout (Oncorhynchus mykiss).
In rainbow trout only a single classical major histocompatibility complex (MHC) class I locus has been identified. In previous studies it was shown that this locus, Onmy-UBA, is characterised by extensive polymorphism and ubiquitous expression. In the present study the sequence of the promoter region of Onmy-UBA was analysed. Within this region several fragments for transcription regulation could be detected, including interferon-sensitive enhancer elements. The promoter region is very similar to that of classical MHC class I in higher vertebrates. In agreement with the interferon sensitive elements identified in its promoter, Onmy-UBA expression in rainbow trout gonad cells (RTG-2 cell line) was up-regulated after infection with infectious haematopoietic necrosis virus (IHNV).
Dijkstra
Johannes Martinus
JM
Inland Station/National Research Institute of Aquaculture, Fisheries Research Agency, Tamaki, Mie, 519-0423, Japan.
Yoshiura
Yasutoshi
Y
Kiryu
Ikunari
I
Aoyagi
Kazuhiko
K
Köllner
Bernd
B
Fischer
Uwe
U
Nakanishi
Teruyuki
T
Ototake
Mitsuru
M
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D005786:Gene Expression Regulation; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000276:immunology; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D015395:Histocompatibility Antigens Class I