Identification and characterization of the peptidic component of the immunomodulatory glycoconjugate Immunoferon.
Inmunoferon is a glycoconjugate of natural origin, formed by the noncovalent association of a protein from Ricinus communis and a polysacharidic moiety, and endowed with immunomodulatory as well as pharmacological activities. This study investigated the nature of polypeptidic component of Inmunoferon. Through biochemical procedures and comparison with protein databases, the isolated protein was identified as the processed form of the seed of Ricinus communis 2S storage polypeptide, which has been termed RicC3. Further analysis of the isolated protein has revealed that it is composed of two different subunits, alpha and beta, which form an heterodimer of high stability and resistance to denaturation, acidic pH and proteolytic cleavage. These findings confirm the excellent properties of the product after oral administration and provide additional support for the pharmacological activities of Inmunoferon.
Varela
J
J
Centro de Investigaciones Biológicas (CIB), Industrial Farmaceútica Cantabria S.A., Madrid, Spain.
Navarro Pico
M L
ML
Guerrero
A
A
García
F
F
Giménez Gallego
G
G
Pivel
J P
JP
D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D000418:Albumins / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D030562:Databases, Protein; D019281:Dimerization; D006001:Glycoconjugates / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D006020:Glycopeptides / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011487:Protein Conformation; D011489:Protein Denaturation; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D012278:Ricinus / Q000737:chemistry; D020539:Sequence Analysis, Protein / Q000379:methods; D017386:Sequence Homology, Amino Acid; D014357:Trypsin / Q000378:metabolism
D000276:Adjuvants, Immunologic; D000418:Albumins; D002130:Calcium Phosphates; D006001:Glycoconjugates; D006020:Glycopeptides; D010455:Peptides; D010940:Plant Proteins; C038773:Immunoferon; D014357:Trypsin