title: A new method to enhance contrast of ultrathin cryosections for immunoelectron microscopy.
abstract: Adequate contrast of ultrathin cryosections is crucial for evaluating morphological detail to assess immunocytochemical localization at the electron microscopic level. We have developed a positive staining method for achieving contrast in ultrathin cryosections, from tissue fixed only in paraformaldehyde, that provides excellent contrast at the electron microscopic level.
authors.affiliation: Department of Physiology, Ohio State University, Columbus, Ohio 43210, USA. takizawa.2@osu.edu
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Takizawa
authors.forename: Clark L
authors.initials: CL
authors.lastname: Anderson
authors.forename: John M
authors.initials: JM
authors.lastname: Robinson
mesh_terms: D051242:Caveolin 1; D022461:Caveolins / Q000032:analysis; D002478:Cells, Cultured; D005404:Fixatives; D005557:Formaldehyde; D005629:Frozen Sections; D006801:Humans; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron / Q000379:methods*; D010920:Placenta / Q000737:chemistry / Q000648:ultrastructure; D011108:Polymers; D013194:Staining and Labeling / Q000379:methods*; D014471:Umbilical Veins / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure
chemical_list: D051242:Caveolin 1; D022461:Caveolins; D005404:Fixatives; D011108:Polymers; D005557:Formaldehyde; C003043:paraform