Context dependence of meiotic recombination hotspots in yeast: the relationship between recombination activity of a reporter construct and base composition.
Borde and colleagues reported that a reporter plasmid inserted at different genomic locations in Saccharomyces cerevisiae had different levels of meiotic recombination activity. We show that the level of recombination activity is very significantly correlated with the GC content of DNA sequences flanking the insertion.
Petes
Thomas D
TD
Department of Biology and Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA. tompetes@email.unc.edu
Merker
Jason D
JD
D001482:Base Composition; D015825:Chromosomes, Fungal / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004274:DNA, Recombinant / Q000737:chemistry / Q000235:genetics; D017930:Genes, Reporter; D008540:Meiosis / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal; D004274:DNA, Recombinant