[Analysis of selected usage and clonal expansion of TCR Vbeta repertoire of peripheral blood T cells in patients with non-Hodgkin's lymphoma].
To investigate the distribution and clonal expansion of TCR Vbeta subfamily T cells in patients with B-NHL and T-NHL, the CDR3 of TCR Vbeta 24 subfamily genes was amplified in peripheral blood mononuclear cells from 4 cases with B-NHL and 2 cases with T-NHL using RT-PCR, and to observe the usage of TCR Vbeta repertoire, the PCR products were further labeled with fluorescence and analyzed by genescan technique for the CDR3 size, to evaluating clonality of the detectable TCR Vbeta T cells. The results indicated that only selected expression of 6-12 Vbeta subfamily T cells could be identified in the 6 cases with NHL, and Vbeta1, Vbeta8, Vbeta13 and Vbeta19 were expressed in all samples, Vbeta2 and Vbeta16 could be found in 5 samples, whereas Vbeta4-6, Vbeta10-12, Vbeta15, Vbeta17-18, Vbeta20 and Vbeta22-23 were absent in all samples. Genescan analysis showed that clonal expansion of T cells could be found in 1-3 Vbeta subfamilies from 2 cases with B-NHL and 1 case with T-NHL. In conclusions, the similar selected usage of TCR Vbeta subfamily T cells could be found in peripheral blood from patients with B and T NHL, clonal expansion of T cells which were considered to be related to lymphoma cell antigen could be detected in a part of patients with B or T NHL.
Lu
Yu-Hong
YH
Department of Hematology, The First Affiliated Hospital, Institute of Hematology, Medical College of Jinan University, Guangzhou 510632, China.
Li
Yang-Qiu
YQ
Yang
Li-Jian
LJ
Chen
Shao-Hua
SH
Zhang
Tao
T
Luo
Geng-Xin
GX
Zhang
Yu-Ping
YP
D002460:Cell Line; D002999:Clone Cells; D022801:Complementarity Determining Regions / Q000235:genetics; D019674:Genes, T-Cell Receptor beta / Q000235:genetics*; D006801:Humans; D019169:Jurkat Cells; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics* / Q000276:immunology; D012334:RNA, Neoplasm / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000378:metabolism*
D022801:Complementarity Determining Regions; D012334:RNA, Neoplasm