Expression imbalance map: a new visualization method for detection of mRNA expression imbalance regions.
We describe the development of a new visualization method, called the expression imbalance map (EIM), for detecting mRNA expression imbalance regions, reflecting genomic losses and gains at a much higher resolution than conventional technologies such as comparative genomic hybridization (CGH). Simple spatial mapping of the microarray expression profiles on chromosomal location provides little information about genomic structure, because mRNA expression levels do not completely reflect genomic copy number and some microarray probes would be of low quality. The EIM, which does not employ arbitrary selection of thresholds in conjunction with hypergeometric distribution-based algorithm, has a high tolerance of these complex factors. The EIM could detect regionally underexpressed or overexpressed genes (called, here, an expression imbalance region) in lung cancer specimens from their gene expression data of oligonucleotide microarray. Many known as well as potential loci with frequent genomic losses or gains were detected as expression imbalance regions by the EIM. Therefore, the EIM should provide the user with further insight into genomic structure through mRNA expression.
Kano
Makoto
M
School of Engineering, University of Tokyo, Tokyo 113-8655, Japan. mkano@jp.ibm.com
Nishimura
Kunihiro
K
Ishikawa
Shumpei
S
Tsutsumi
Shuichi
S
Hirota
Koichi
K
Hirose
Michitaka
M
Aburatani
Hiroyuki
H
D022981:Allelic Imbalance / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D002874:Chromosome Mapping / Q000379:methods* / Q000706:statistics & numerical data; D016000:Cluster Analysis; D005784:Gene Amplification / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods* / Q000706:statistics & numerical data; D023061:Gene Order / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000706:statistics & numerical data; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger