Localization of Nav1.5 sodium channel protein in the mouse brain.
Na(v)1.5 or SCN5A is a member of the voltage-dependent family of sodium channels. The distribution of Na(v)1.5 protein was investigated in the mouse brain using immunohistochemistry. Immunostaining with a Na(v)1.5-specific antibody revealed that Na(v)1.5 protein was localized in certain distinct regions of brain including the cerebral cortex, thalamus, hypothalamus, basal ganglia, cerebellum and brain stem. Notably, we found that Na(v)1.5 protein co-localized with neurofilaments and clustered at a high density in the neuronal processes, mainly axons. These results suggest that Na(v)1.5 protein may play a role in the physiology of the central nervous system (generation and propagation of electrical signals by axons).
Wu
Ling
L
Cole Eye Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
Nishiyama
Kazutoshi
K
Hollyfield
Joe G
JG
Wang
Qing
Q
D000818:Animals; D000918:Antibody Specificity; D001923:Brain Chemistry; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009124:Muscle Proteins / Q000032:analysis / Q000276:immunology; D062554:NAV1.5 Voltage-Gated Sodium Channel; D015222:Sodium Channels / Q000032:analysis* / Q000276:immunology
D009124:Muscle Proteins; D062554:NAV1.5 Voltage-Gated Sodium Channel; C568321:Scn5a protein, mouse; D015222:Sodium Channels