Effects of octylphenol and 17beta-estradiol on the gonads of guppies (Poecilia reticulata) exposed as adults via the water or as embryos via the mother.
Endocrine disrupting alkylphenolic compounds have been found in the aquatic environment, and concern has arisen over the ability of these compounds to affect the reproductive system of fish. In this study, the effects of exposure to an environmentally relevant concentration of octylphenol or 17beta-estradiol on the gonad structure of fish were examined. Viviparous guppies (Poecilia reticulata) were exposed as adults via the water or as embryos via the mother to 26 microg/l octylphenol or 0.85 microg/l 17beta-estradiol (mean measured water concentrations). Histological examinations revealed effects of the exposures on the gonads of the fish exposed as adults. Indications of blocked spermatogonial mitosis were seen in the testis structure of adult males after exposure to octylphenol or 17beta-estradiol. The post-parturition ovaries of adult females exposed to 17beta-estradiol showed effects suggesting an inhibited yolk deposition. At the tested concentrations, exposure to octylphenol or 17beta-estradiol via the mother fish did not significantly affect the weight, length, gonopodium index or sex distribution of the offspring. However, histology revealed effects on the liver structure, suggesting some effect of maternal exposure to octylphenol or 17beta-estradiol. These findings indicate that although octylphenol and 17beta-estradiol affect the gonad structure of adult male and female guppies, these substances have no significant effects on the sexual differentiation of the embryos at the tested concentrations.
Kinnberg
Karin
K
Danish Centre for Environmental Oestrogen Research, Institute of Biology, University of Southern Denmark--Odense, Campusvej 55, DK-5230 Odense M, Denmark. kinnberg@biology.sdu.dk
Korsgaard
Bodil
B
Bjerregaard
Poul
P
D000818:Animals; D004625:Embryo, Nonmammalian / Q000002:abnormalities / Q000187:drug effects*; D004958:Estradiol / Q000633:toxicity*; D005260:Female; D006066:Gonads / Q000002:abnormalities / Q000187:drug effects* / Q000473:pathology; D006727:Hormone Antagonists / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D018811:Maternal Exposure; D009866:Oogenesis / Q000187:drug effects; D010053:Ovary / Q000002:abnormalities / Q000187:drug effects / Q000473:pathology; D010636:Phenols / Q000633:toxicity*; D011036:Poecilia; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000002:abnormalities / Q000187:drug effects / Q000473:pathology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D006727:Hormone Antagonists; D010636:Phenols; D014874:Water Pollutants, Chemical; C474055:octylphenol; D004958:Estradiol