Biodefense-driven murine model of pneumonic melioidosis.
A whole-body mouse model of pneumonic melioidosis was established for future evaluation of biodefense vaccine candidates. The aerosol 50% lethal doses of Burkholderia pseudomallei strain 1026b for BALB/c and C57BL/6 mice and the times to death, dissemination in organs, and tissue loads after exposure of the mice to low- and high-dose aerosols are reported. In addition, rpsL mutant backgrounds were attenuated in this acute model of disease.
Jeddeloh
J A
JA
Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 21702-5011, USA. jjeddeloh@oriongenomics.com
Fritz
D L
DL
Waag
D M
DM
Hartings
J M
JM
Andrews
G P
GP
D000818:Animals; D023482:Bioterrorism / Q000517:prevention & control*; D016957:Burkholderia pseudomallei / Q000472:pathogenicity*; D004195:Disease Models, Animal; D006801:Humans; D008554:Melioidosis / Q000382:microbiology / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018410:Pneumonia, Bacterial / Q000382:microbiology / Q000473:pathology*; D012269:Ribosomal Proteins / Q000235:genetics; D014774:Virulence
D012269:Ribosomal Proteins; C034212:ribosomal protein S12