Thermo-acido-tolerant phytase production from a soil bacterium in a medium containing rice bran and soybean meal extract.
A bacterial strain capable of producing a thermo-acido-tolerant phytase was isolated from soil around haystacks and designated as strain PH01. The phytase produced was purified to homogeneity as determined by native PAGE. From SDS-PAGE, it was 30 kDa in size. The purified phytase was a thermo-acido-tolerant enzyme. A complex medium for the PH01 phytase production was developed. The medium, "PheB", was composed of 2% glucose, 0.2% CaCl(2), 0.5% NH(4)NO(3), 0.05% KCl, 0.05% MgSO(4).7H(2)O, 0.001% FeSO(4).7H(2)O, 0.001% MnSO(4).H(2)O in rice bran plus soybean meal extract containing 3% (v/v) phosphate solution (7.3% NaHPO(4)+3.2%KH(2)PO(4), pH 7.2). Cultivation was done at 37 degrees C with aeration for 48 h which produced phytase at 10 U/ml. Exposure of the phytase to 1% bile salt; i.e., taurocholate or deoxycholate, caused less than 15% reduction of activity. Potential application of PH01 phytase as a feed supplement was suggested.
Popanich
Suthiluk
S
Centre for Biotechnology, Institute for Research and Development in Science and Technology, Mahidol University, Buddhamonthon 4 Rd., Salaya, Nakornpathom 73170, Thailand.
Klomsiri
Chananart
C
Dharmsthiti
Saovanee
S
D010832:6-Phytase / Q000096:biosynthesis / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D012275:Oryza; D012037:Refuse Disposal; D012988:Soil Microbiology; D013025:Glycine max; D013696:Temperature
D010832:6-Phytase