[Effects of exogenous adenosine 5'-monophosphate on proliferation and apoptosis of small intestinal epithelial cells].
The effect of exogenous adenosine 5'-monophosphate (AMP) on proliferation and apoptosis of a normal rat small intestinal epithelial cell line, IEC-6, was investigated. Proliferation of IEC-6 was measured by MTT. Cell cycle and apoptosis was determined by flow cytometry. Expression of Bax was detected by immunohistochemistry. The results showed that AMP remarkably inhibited the proliferation of IEC-6 in a dose- and time-dependent way. AMP also significantly changed the distribution of cell cycle, arrested cells in S phase and enhanced apoptosis by inducing Bax expression.
Chen
X
X
School of Public Health, West China University of Medical Science, Chengdu 610041, China.
Wang
R
R
Deng
M
M
Ran
X
X
D000249:Adenosine Monophosphate / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology*; D007421:Intestine, Small / Q000166:cytology*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2; D051381:Rats; D051028:bcl-2-Associated X Protein
C494828:Bax protein, rat; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D000249:Adenosine Monophosphate