[Apoptosis of human lung carcinoma cell line GLC-82 induced by high power electromagnetic pulse].
OBJECTIVE
Electromagnetic pulse (EMP) could be used for sterilization of food and the efficiency is higher than 2450 MHz continuous microwave done. This study was designed to evaluate the effect of electromagnetic pulse (EMP) on apoptosis of human lung carcinoma cell line GLC-82, so that to explore and develop therapeutic means for cancer.


METHODS
The injury changes in GLC-82 cells after irradiated with EMP (electric field intensity was 60 kV/m, 5 pulses/2 min) were analyzed by cytometry, MTT chronometry, and flow cytometry. The immunohistochemical SP staining was used to determine the expressions of bcl-2 protein and p53 protein. The stained positive cells were analyzed by CMIAS-II image analysis system at a magnification 400. All data were analyzed by SPSS8.0 software.


RESULTS
EMP could obviously inhibited proliferation and activity of lung carcinoma cell line GLC-82. The absorbance value (A570) of MTT decreased immediately, at 0 h, 1 h, and 6 h after the GLC-82 cells irradiated by EMP as compared with control group. The highest apoptosis rate was found to reach 13.38% by flow cytometry at 6 h after EMP irradiation. Down-regulation of bcl-2 expression and up-regulation of p53 expression were induced by EMP.


CONCLUSIONS
EMP promotes apoptosis of GLC-82 cells. At same time, EMP can down-regulate bcl-2 expression and up-regulate p53 expression in GLC-82 cells. The bcl-2 and the p53 protein may involve the apoptotic process.
Cao
Xiao-zhe
XZ
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, P. R. China. xiaozhecao@yahoo.com
Zhao
Mei-lan
ML
Wang
De-wen
DW
Dong
Bo
B
D017209:Apoptosis / Q000528:radiation effects*; D002455:Cell Division / Q000528:radiation effects; D055590:Electromagnetic Phenomena; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism / Q000528:radiation effects; D013997:Time Factors; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism / Q000528:radiation effects
D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53