title: Significance of monocyte chemotactic protein-1 and thymidine phosphorylase in angiogenesis of human cardiac myxoma.
abstract: Angiogenesis is indispensable to tumor development and proliferation. The aim of this study was to investigate whether the expression of monocyte chemotactic protein-1 (MCP-1) and of thymidine phosphorylase (TP) correlates with the angiogenesis and clinicopathologic features in cardiac myxoma. Paraffin-embedded specimens of 17 resected cardiac myxomas were immunohistochemically stained for MCP-1, CC chemokine receptor-2 (CCR-2), TP, CD31, and CD68. Correlations among MCP-1 expression, TP expression, microvessel count (determined by CD31 staining), macrophage count (determined by CD68 staining), and the clinicopathologic features of the patients were analyzed statistically. Immunohistochemical analysis revealed that MCP-1 and TP were expressed in myxoma cells, as well as in stromal cells such as infiltrating cells, fibroblast-like cells and endothelial cells. CCR-2 was abundantly expressed in stromal infiltrating cells in all myxomas and occasionally in the endothelial cells. In the tumor stroma, the major source of MCP-1, TP and CCR-2 was macrophages, and the sites of positive staining for MCP-1, TP and CCR-2 matched in most of the myxomas. Statistical analysis revealed that the proportions of MCP-1-positive myxoma and stromal cells, and TP-positive myxoma and stromal cells significantly correlated with increased microvessel count. The proportions of MCP-1-positive myxoma and stromal cells significantly correlated with the proportion of TP-positive stromal cells. The mean microvessel count in myxomas with both high tumor and high stromal MCP-1 or TP expression was significantly higher than that in myxomas with low tumor and low stromal MCP-1 or TP expression. Small tumors (< or =55 mm in diameter) exhibited high MCP-1 or TP expression, and the microvessel count in small tumors was significantly higher than in large myxomas. Although the difference was not significant, myxomas with both high tumor and high stromal MCP-1 expression had a higher macrophage count than other myxomas. These results indicate that in cardiac myxoma, MCP-1 and TP may be regarded as important angiogenic signals accompanying growth.
authors.affiliation: Second Department of Surgery, Shinshu University School of Medicine, Asahi, Matsumoto, Japan.
authors.forename: Tianshu
authors.initials: T
authors.lastname: Zhang
authors.forename: Naohiko
authors.initials: N
authors.lastname: Koide
authors.forename: Yuko
authors.initials: Y
authors.lastname: Wada
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Tsukioka
authors.forename: Kei
authors.initials: K
authors.lastname: Takayama
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kono
authors.forename: Hiroto
authors.initials: H
authors.lastname: Kitahara
authors.forename: Jun
authors.initials: J
authors.lastname: Amano
mesh_terms: D001808:Blood Vessels / Q000473:pathology; D002452:Cell Count; D018932:Chemokine CCL2 / Q000378:metabolism*; D006338:Heart Neoplasms / Q000150:complications* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008264:Macrophages / Q000473:pathology; D008833:Microcirculation; D008875:Middle Aged; D009232:Myxoma / Q000150:complications* / Q000378:metabolism* / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000209:etiology*; D054390:Receptors, CCR2; D019707:Receptors, Chemokine / Q000378:metabolism; D013194:Staining and Labeling; D013939:Thymidine Phosphorylase / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: C515334:CCR2 protein, human; D018932:Chemokine CCL2; D054390:Receptors, CCR2; D019707:Receptors, Chemokine; D013939:Thymidine Phosphorylase