[A colorimetric method of HEC toxin hemolysis test for diagnosis of paroxysmal nocturnal hemoglobinuria].
The purpose of the study is to establish a colorimetric method of HEC toxin hemolysis test for diagnosis of paroxysmal nocturnal hemoglobinuria (PNH). RBCs from normal persons and patients with PNH and non-PNH anemia were treated with HEC toxin secreted by Aeromonas hydrophila J-1 strain and the absorbance at 630 nm was measured to quantitate the extent of hemolysis. The results demonstrated that the RBCs from PNH patients showed resistance to the toxin hemolysis, which was in accord with the percentages of CD59(-) cells, while the RBCs from normal persons and non-PNH anemic patients were nearly totally lysed. It is concluded that the method can be considered as a simple, specific and reliable method for the diagnosis of PNH.
Yang
Yang
Y
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Faculty of Basic Medicine of Peking Union Medical College, Beijing 100005, China.
Xu
Cai-Min
CM
Wang
Jian
J
Chen
Lan
L
Pan
Hua-Zhen
HZ
Zhang
Zhi-Nan
ZN
D001427:Bacterial Toxins / Q000633:toxicity*; D003124:Colorimetry; D005434:Flow Cytometry; D006457:Hemoglobinuria, Paroxysmal / Q000175:diagnosis*; D006461:Hemolysis; D006801:Humans
D001427:Bacterial Toxins; C475608:HEC toxin, Aeromonas hydrophila