[Studies on the metabolism of gentiopicroside by rat intestinal flora].
OBJECTIVE
To study the metabolism of gentiopicroside by rat intestinal flora in vitro and time profiles of gentiopicroside and its metabolites in rat serum after oral administration of gentiopicroside.


METHODS
Using high performance liquid chromatography (HPLC) for determination of gentiopicroside, gentianine and gentianal content of rat intestinal flora culture medium in vitro and rat serum sample in vivo.


RESULTS
Gentiopicroside was metabolized quickly in vitro by rat intestinal flora, consequently gentianine and gentianal content appeared. Gentianine and gentianal were observed after gentiopicroside oral administration 4 times in rat serum sample, and gentiopicroside content decreased after oral administration 6 times.


CONCLUSIONS
Gentianine and gentianal were the important bioactive metabolites of gentiopicroside in rats.
Yang
X F
XF
Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Song
C Q
CQ
D000470:Alkaloids / Q000096:biosynthesis / Q000097:blood; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D030012:Gentiana / Q000737:chemistry; D005960:Glucosides / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology; D057888:Iridoid Glucosides; D039823:Iridoids / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D008297:Male; D011714:Pyrans / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D000470:Alkaloids; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans; C012997:gentiopicroside; C060402:gentianine; C060403:gentianal