GD3 expression in CHO-K1 cells increases growth rate, induces morphological changes, and affects cell-substrate interactions.
We have generated a panel of CHO-K1 cell clones with different glycolipid compositions by stable transfection of appropriate glycosyltransferases and studied the morphological and growth phenotype of a clone stably expressing Sial-T2. Compared with the GM3 expressing parental cells, Sial-T2 transfectants show low expression of GM3 and neo expression of GD3 and GT3. These cells show about 60% reduction of the mean cell area, and about 2-fold increase of the mean colony area and growth rate. Cells over expressing Sial-T2 showed a flattened appearance, and with time in culture they detached from the substrate leaving adhered material that was GD3 immunoreactive. No apoptotic or proteome differences could be detected in the Sial-T2 transfectants. Thus, increased expression of GD3 and GT3 influence parameters of growth and social behavior of CHO-K1 cells. However, the molecular and cellular basis underlying these influences requires further investigation.
Daniotti
Jose L
JL
CIQUIBIC (UNC-CONICET), Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
Zurita
Adolfo R
AR
Trindade
Vera M T
VM
Maccioni
Hugo J F
HJ
D000818:Animals; D016466:CHO Cells; D002455:Cell Division; D002999:Clone Cells; D006224:Cricetinae; D005732:Gangliosides / Q000378:metabolism*; D020543:Proteome
D005732:Gangliosides; D020543:Proteome; C026226:ganglioside, GD3