Valency of antibody binding to enveloped virus particles as determined by surface plasmon resonance.
A simple method is described for determining the valency of binding of immunoglobulin G to immobilized influenza A virus. Where there is a free Fab arm (monovalent binding), a second virus particle is captured. This is detected by surface plasmon resonance. The methodology should be applicable to all enveloped and nonenveloped viruses.
Hardy
Sam A
SA
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom.
Dimmock
Nigel J
NJ
D001666:Binding Sites, Antibody; D015374:Biosensing Techniques; D007074:Immunoglobulin G / Q000378:metabolism*; D009980:Influenza A virus / Q000378:metabolism*; D020349:Surface Plasmon Resonance
D007074:Immunoglobulin G