Inhibitory effect of the water-soluble extract of Salvia miltiorrhiza on neutrophil-endothelial adhesion.
The effect of the water-soluble extract (WSE) of Salvia miltiorrhiza on neutrophil-endothelial cell adhesion was investigated. Cell adhesion was evaluated by testing neutrophil myeloperoxidase activity: expression of adhesion molecules in human umbilical vein endothelial cells (HUVEC) was measured by ELISA: the neutrophil activation rate induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP) was tested by the method of nitroblue tetrazolium (NBT) reduction. The results showed that the adhesion rate of neutrophils to unstimulated HUVEC was very low. TNFalpha (50 - 800 U/ml) increased the adhesion of neutrophils to TNFalpha-stimulated HUVEC in a concentration- and time-dependent manner. The WSE of Salvia miltiorrhiza (0.01 - 1 mg/ml) dose-dependently inhibited the adhesion of neutrophils. The inhibitory rate of the WSE of Salvia miltiorrhiza at 0.01, 0.1 and 1 mg/ml was 6.2%, 17.0% and 28.0%, respectively. fMLP (10(-9) - 10(-5) M) increased the activation rate of neutrophils concentration-dependently. The WSE of Salvia miltiorrhiza also concentration-dependently inhibited the adhesion of fMLP-activated neutrophils to HUVEC. The inhibitory rate of the WSE of Salvia miltiorrhiza at 0.001, 0.01 and 0.1 mg/ml was 5.3%, 26.3% and 28.9%, respectively. Moreover, TNFalpha upregulated expression of adhesion molecule E-selectin, ICAM-1 and VCAM-1. The WSE of Salvia miltiorrhiza had an inhibitory effect on TNF alpha-induced expression of these molecules. These results indicated that the WSE of Salvia miltiorrhiza inhibited neutrophil-endothelial adhesion. The action mechanism of the WSE of Salvia miltiorrhiza was partly related to suppressing the expression of adhesion molecules.
Ren
De Cheng
DC
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Du
Guan Hua
GH
Zhang
Jun Tian
JT
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D018375:Neutrophil Activation; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D018517:Plant Roots; D051381:Rats; D017208:Rats, Wistar; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects; D014867:Water
D000893:Anti-Inflammatory Agents; D015815:Cell Adhesion Molecules; D004365:Drugs, Chinese Herbal; D014409:Tumor Necrosis Factor-alpha; D014867:Water; D009240:N-Formylmethionine Leucyl-Phenylalanine