Monomeric sarcoplasmic reticulum Ca(2+)-ATPase is functionally active.
cRNAs for wild type and a functionless mutant of sarcoplasmic reticulum (SR) Ca(2+)-ATPase were co-injected into Xenopus oocytes in various molar ratios. SR Ca(2+)-ATPase activity of the microsomal fraction prepared from the injected oocytes increased linearly with increasing amounts of wild type cRNA. This result suggests that monomers are functional units of membrane-bound SR Ca(2+)-ATPase.
Takeda
Kazuo
K
Department of Biology, University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
Kawamura
Masaru
M
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D018075:RNA, Complementary / Q000096:biosynthesis; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D014981:Xenopus
D018075:RNA, Complementary; D000252:Calcium-Transporting ATPases