[CD62p expression in platelet during the preparation course of Cryopreservated platelet-rich plasma].
In order to explore the factors that affect CD62p expression in platelet during the whole course of cryopreservated platelets preparation, CD62p expression of platelet was evaluated by flow cytometry assay. The whole course of cryopreservated platelets preparation in order included whole blood collection, centrifugation for fresh platelet-rich plasma preparation, addition of dimethyl sulfoxide, and freeze in -80 degrees C refrigerator and thaw in 38 degrees C water bath. Result showed that the CD62p expressed slowly from whole blood collection to addition of dimethyl sulfoxide, but expressed abruptly after freeze and thaw and it occupied 82 per cent of the whole expression. It was concluded that the whole blood collection, centrifugation for fresh platelet enriched plasma preparation and addition of dimethyl sulfoxide were optimized in the whole course, but the damage to platelets in the whole course of cryopreservation could not be avoided. It suggests that improved techniques are needed to reduce the damage to cryopreservated platelets.
Liu
Jing-Han
JH
Department of Blood Transfusion, The General Hospital of PLA, Centre for Clinical Blood Transfusion Medicine of PLA, Beijing 100853, China.
Ouyang
Xi-Lin
XL
Shi
Qun
Q
Luo
Qun
Q
Li
Xi-Jin
XJ
Wang
Hai-Bao
HB
Cheng
Min-Cai
MC
Han
Wei
W
Gao
Dayong
D
D001792:Blood Platelets / Q000378:metabolism*; D001793:Blood Preservation / Q000379:methods* / Q000592:standards; D015925:Cryopreservation / Q000379:methods* / Q000592:standards; D005434:Flow Cytometry; D006801:Humans; D019007:P-Selectin / Q000096:biosynthesis*
D019007:P-Selectin