[The study on in vivo erythropoietin gene transfer for treatment of renal anemia in animal model].
To explore the possibility of gene therapy to renal anemia, the receptor mediated erythropoietin (EPO) gene transfer in vivo had been tested. EPO gene was inserted to an EBV replicon expression vector to form the plasmid pEPO, Renal anemia rat had been induced by feeding with adenine, and 450 micrograms of the pEPO was delivered by perfusion into the rat peripheral circulation in the form of soluble DNA/galactosylate histone complex. 13 days after injection, the number of peripheral red cells was counted and the concentration of hemoglobin was measured. The results demonstrated that symptom of anemia had been significantly improved(P < 0.01), when compared with that of the control test. (the red cell number was 492 vs 409, and the hemoglobin was 11.4 vs 9.1).
Sui
B
B
National Laboratory of Molecular Virology and Genetic Engineering, Beijing 100052.
Han
F
F
Wu
S
S
D000740:Anemia / Q000628:therapy*; D000818:Animals; D004274:DNA, Recombinant / Q000235:genetics; D004921:Erythropoietin / Q000096:biosynthesis / Q000235:genetics* / Q000627:therapeutic use*; D018014:Gene Transfer Techniques; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins
D004274:DNA, Recombinant; D011994:Recombinant Proteins; D004921:Erythropoietin