Potentiation of nerve growth factor-induced neurite outgrowth in PC12 cells by a Coptidis Rhizoma extract and protoberberine alkaloids.
A methanol extract of Coptidis Rhizoma effectively enhanced the outgrowth of neurite in PC12 cells induced by nerve growth factor (NGF). Following solvent partition and preparative HPLC, berberine was isolated as the major active compound. Berberine enhanced the proportion of neurite-bearing cells in a dose-dependent manner without cytotoxicity. Its structural relatives, palmatine and coptisine, showed a slightly weaker NGF-enhancing effect than berberine. These three alkaloids inhibited acetylcholinesterase activity at a level comparable to that of physostigmine, but this inhibition was not responsible for the potentiation of NGF-induced neurite outgrowth. It is demonstrated for the first time that protoberberine alkaloids potentiated the NGF-induced differentiation of neural cells.
Shigeta
Koji
K
School of Bioresources, Hiroshima Prefectural University, 562 Nanatsuka, Shobara 727-0023, Japan.
Ootaki
Keisuke
K
Tatemoto
Hideki
H
Nakanishi
Tsutomu
T
Inada
Akira
A
Muto
Norio
N
D000818:Animals; D001599:Berberine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001600:Berberine Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000089042:Coptis chinensis; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology; D004789:Enzyme Activation / Q000502:physiology; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D016716:PC12 Cells; D051381:Rats
D001600:Berberine Alkaloids; D002800:Cholinesterase Inhibitors; D004365:Drugs, Chinese Herbal; D009414:Nerve Growth Factors; C034384:coptisine; D001599:Berberine; C009090:protoberberine; C107393:Coptidis rhizoma extract; C005413:palmatine