title: [Study on the effect of a biostimulant on the growth and toxigenic function of Clostridium tetani strain Copenhagen-471].
abstract: Bakstim, a new biostimulating preparation obtained from the organs of the immune system of animals, was developed. The impact of Bakstim on the growth and toxigenic function of C. tetani production strain Copenhagen-471 was evaluated. The addition of the preparation to Gluzman commercial medium for obtaining tetanus toxoid led to an increase in the yield of bacterial biomass from 1.9 to 4-fold and an increase in the toxoid production from 2 to 2.8-fold. The optimum concentration of this biostimulant ensuring the maximum yield of tetanus toxin from the production culture was determined (1,000 mg/l). Bakstim will supposedly be used as additive to nutrient media for the production of tetanus toxoid.
authors.affiliation: Research Institute for Vaccines and Sera, Tashkent, Uzbekistan.
authors.forename: F Iu
authors.initials: FIu
authors.lastname: Garib
authors.forename: V Iu
authors.initials: VIu
authors.lastname: Petrov
authors.forename: E A
authors.initials: EA
authors.lastname: Komarova
authors.forename: N N
authors.initials: NN
authors.lastname: Sheremet'ev
mesh_terms: D001426:Bacterial Proteins / Q000494:pharmacology*; D001685:Biological Factors / Q000302:isolation & purification / Q000494:pharmacology*; D018533:Biomass; D003017:Clostridium tetani / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D003470:Culture Media; D005069:Evaluation Studies as Topic; D007107:Immune System / Q000737:chemistry; D013744:Tetanus Toxin / Q000096:biosynthesis*; D013745:Tetanus Toxoid / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D001685:Biological Factors; D003470:Culture Media; D013744:Tetanus Toxin; D013745:Tetanus Toxoid