Separation and determination of nitrobenzenes by micellar electrokinetic chromatography and high-performance liquid chromatography.
A micellar electrokinetic chromatographic method and a high-performance liquid chromatographic method are proposed for the separation and determination of a mixture of 12 nitrobenzenes and their reduction products, namely 4-nitro-1,2-phenylenediamine, 4-nitro-1,3-phenylenediamine, 2-nitro-1,4-phenylenediamine, 2-nitroaniline, 3-nitroaniline, 4-nitroaniline, 4-amino-2-nitrophenol, 2-amino-5-nitrophenol, 2-amino-4-nitrophenol, 2-nitrophenol, 3-nitrophenol, and 4-nitrophenol. A solution of 50 mM sodium dodecyl sulfate and 10% ethanol in 23 mM sodium borate buffer was used as the electrophoretic medium. Good resolution could be obtained by the addition of tetrahydrofuran to the liquid chromatographic mobile phase. The retention and migration behavior of the nitrobenzenes are discussed.
Wang
Shu-Ping
SP
Department of Applied Chemistry, Providence University, 200 Chunchi Road, Shalu, Taichung Hsien 43301, Taiwan. spwang@pu.edu.tw
Chen
Hwei-Jun
HJ
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006202:Hair Dyes / Q000737:chemistry; D009578:Nitrobenzenes / Q000737:chemistry / Q000302:isolation & purification*
D006202:Hair Dyes; D009578:Nitrobenzenes