Glutathione peroxidase-1 contributes to the neuroprotection seen in the superoxide dismutase-1 transgenic mouse in response to ischemia/reperfusion injury.
The authors hypothesized that glutathione peroxidase-1 (Gpx-1) contributes to the neuroprotection seen in the superoxide dismutase-1 transgenic (Sod-1 tg) mouse. To investigate this hypothesis, they crossed the Gpx-1 -/- mouse with the Sod-1 tg and subjected the cross to a mouse model of ischemia/reperfusion. Two hours of focal cerebral ischemia followed by 24 hours of reperfusion was induced via intraluminal suture. The Sod-1 tg/Gpx-1 -/- cross exhibited no neuroprotection when infarct volume was measured; indeed, infarct volume increased in the Sod-1 tg/Gpx-1 -/- cross compared with the wild-type mouse. Our results suggest that Gpx-1 plays an important regulatory role in the protection of neural cells in response to ischemia/reperfusion injury.
Crack
Peter J
PJ
Center for Functional Genomics and Human Disease, Monash Institute of Reproduction and Development, Monash University, Melbourne, Australia. peter.crack@med.monash.edu.au
Taylor
Juliet M
JM
de Haan
Judy B
JB
Kola
Ismail
I
Hertzog
Paul
P
Iannello
Rocco C
RC
D000818:Animals; D001921:Brain / Q000473:pathology; D002545:Brain Ischemia / Q000201:enzymology* / Q000473:pathology*; D002544:Cerebral Infarction / Q000473:pathology; D005979:Glutathione Peroxidase / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics; D008822:Mice, Transgenic / Q000235:genetics; D018696:Neuroprotective Agents; D015427:Reperfusion Injury / Q000201:enzymology* / Q000473:pathology*; D013482:Superoxide Dismutase / Q000502:physiology*; D000072105:Superoxide Dismutase-1; D000094765:Glutathione Peroxidase GPX1
D018696:Neuroprotective Agents; C000606290:SOD1 protein, human; D005979:Glutathione Peroxidase; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D000094765:Glutathione Peroxidase GPX1; C000722068:Gpx1 protein, mouse