title: [Characterization of thermophilic spore-forming bacteria from a geothermal spring in Lithuania based on 16S rDNA and 16S-23S rDNA intergenic spacers analyses].
abstract: Forty-two strains of gram-positive, aerobic, heterotrophic, obligately thermophilic, spore-forming bacteria were isolated from a geothermal site near the Baltic Sea in Lithuania. All of the strains were able to hydrolyze collagen and/or casein. Since characteristics of proteolytic activity are correlated with taxonomic positions of bacteria, the strains were grouped on the basis of molecular biological analyses. On the basis of RFLP patterns of 16S rDNA and 16S-23S rDNA ITS-PCR analysis, the strains were subdivided into nine groups.
authors.affiliation: Department of Plant Physiology and Microbiology, Faculty of Natural Sciences, Vilnius University, Chiurlionio 21/27, Vilnius, 2009 Lithuania. nomeda.kuisiene@gf.vu.lt
authors.forename: N
authors.initials: N
authors.lastname: Kuĭsene
authors.forename: R
authors.initials: R
authors.lastname: Emantene
authors.forename: D
authors.initials: D
authors.lastname: Valiunas
authors.forename: D
authors.initials: D
authors.lastname: Chitavichus
mesh_terms: D002364:Caseins / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis*; D021903:DNA, Ribosomal Spacer / Q000032:analysis*; D016936:Gram-Positive Endospore-Forming Bacteria / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D008097:Lithuania; D010447:Peptide Hydrolases; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D014871:Water Microbiology
chemical_list: D002364:Caseins; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D003094:Collagen; D010447:Peptide Hydrolases