title: Characterization and imprinting status of OBPH1/Obph1 gene: implications for an extended imprinting domain in human and mouse.
abstract: Human 11p15.5, as well as its orthologous mouse 7F4/F5, is known as the imprinting domain extending from IPL/Ipl to H19. OBPH1 and Obph1 are located beyond the presumed imprinting boundary on the IPL/Ipl side. We determined full-length cDNAs and complete genomic structures of both orthologues. We also investigated their precise imprinting and methylation status. The orthologues resembled each other in genomic structure and in the position of the 5' CpG island and were expressed ubiquitously. OBPH1 and Obph1 were predominantly expressed from the maternal allele only in placenta, with hypo- and not differentially methylated 5' CpG islands in both species. These results suggested that the imprinting domain would extend beyond the presumed imprinting boundary and that methylation of the 5' CpG island was not associated with the imprinting status in either species. It remains to be elucidated whether the gene is under the control of the KIP2/LIT1 subdomain or is regulated by a specific mechanism. Analysis of the precise genomic sequence around the region should help resolve this question.
authors.affiliation: Department of Biomolecular Sciences, Saga Medical School, 5-1-1 Nabeshima, Saga, 849-8501, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Higashimoto
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Soejima
authors.forename: Hitomi
authors.initials: H
authors.lastname: Yatsuki
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Joh
authors.forename: Michiko
authors.initials: M
authors.lastname: Uchiyama
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Obata
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Ono
authors.forename: Youdong
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhenghan
authors.initials: Z
authors.lastname: Xin
authors.forename: Xike
authors.initials: X
authors.lastname: Zhu
authors.forename: Sadahiko
authors.initials: S
authors.lastname: Masuko
authors.forename: Fumitoshi
authors.initials: F
authors.lastname: Ishino
authors.forename: Izuho
authors.initials: I
authors.lastname: Hatada
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Jinno
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Iwasaka
authors.forename: Takeshi
authors.initials: T
authors.lastname: Katsuki
authors.forename: Tsunehiro
authors.initials: T
authors.lastname: Mukai
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D018899:CpG Islands / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D005260:Female; D015870:Gene Expression; D018392:Genomic Imprinting; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D011987:Receptors, Steroid / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D026801:Synteny; D000098825:Oxysterol Binding Proteins
chemical_list: D011987:Receptors, Steroid; D000098825:Oxysterol Binding Proteins; D004247:DNA