Changes of ATP and ADP in cultured astrocytes under and after in vitro ischemia.
A very large body of evidence from in vivo studies has been accumulated on a link between the change of energy and cell survival/apoptosis. Using an in vitro ischemia model, we have previously shown that ischemia could induce apoptosis in astrocytes. In this study, we utilized the same in vitro model to investigate changes in ATP and ADP levels in cultured astrocytes and attempted to demonstrate an energy-cell death linkage. Astrocytes remained unaltered after 2 hr of ischemia but were moderately or severely damaged after 4 or 6-8 hr, respectively. The astrocytes that survived various lengths of in vitro ischemic incubation retained their ability to produce ATP after ischemia. Both ATP and ADP levels were increased in astrocytes that remained alive under in vitro ischemia for over 6 hr. The largest decline in the percent of viable astrocytes during ischemia corresponded well to the reduction in ATP and ADP levels in these cultures.
Yu
Albert Cheung Hoi
AC
Neuroscience Research Institute and Department of Neurobiology, Peking University, Beijing, China. achy@dnachip.com.hk
Lau
Anson Ming Fung
AM
Fu
Ada Wing Yu
AW
Lau
Lok Ting
LT
Lam
Philip Yeung
PY
Chen
Xiao Qian
XQ
Xu
Zhen Yu
ZY
D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D016923:Cell Death; D002470:Cell Survival; D002478:Cells, Cultured; D051381:Rats; D017207:Rats, Sprague-Dawley
D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate