Overproduction of alpha-glucosidase in Aspergillus niger transformed with the cloned gene aglA.
We have transformed an industrial strain, Aspergillus niger GN-3, with the alpha-glucosidase gene (aglA) from the same strain. Southern hybridization analysis revealed that transformants had multiple copies of the cloned DNA inserted into the host genome. An 11-fold improvement of enzyme production was achieved by transformation with a DNA fragment composed of 1.11 kb of the 5' noncoding region, 3.12 kb of the coding region containing three introns, and 1.2 kb of the 3' noncoding region. It was found that the 3' noncoding region (1.2 kb) was preferable for maximum production of the enzyme in the transformant.
Lee
Dong-Gun
DG
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo113-8657, Japan.
Nishimura-Masuda
Ikuko
I
Nakamura
Akira
A
Hidaka
Makoto
M
Masaki
Haruhiko
H
Uozumi
Takeshi
T