title: [Studies on method for the determination of kaerophyllin in Bupleurum smithii wolff Wolff var. parvifolium].
abstract: OBJECTIVE
  To establish a quantitative method for the determination of kaerophyllin in Chinese medicine Bupleurum smithii ver. parvifolium.
  
  
  METHODS
  Kaerophyllin was determined by HPLC, the mobile phase consisted of methanol-water(70:30), the flow rate was 1 ml.min-1, the eluate was monitored with UV detector at 328 nm.
  
  
  RESULTS
  The average recovery was 97.1% with RSD = 2.15 (n = 5).
  
  
  CONCLUSIONS
  The result was precise and stable. This method was sensitive and its repeatability was excellent. This method may serve determination of kaerophyllin in B. smithii var. parvifolium.
authors.affiliation: School of Pharmaceutical Sciencn, West China University of Medical Sciences, Chengdu 610041, Sichuan, China.
authors.forename: H S
authors.initials: HS
authors.lastname: Dou
authors.forename: S X
authors.initials: SX
authors.lastname: Zeng
authors.forename: Y H
authors.initials: YH
authors.lastname: Wang
authors.forename: M Y
authors.initials: MY
authors.lastname: Shang
mesh_terms: D029972:Bupleurum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
chemical_list: D004365:Drugs, Chinese Herbal