Plectin-like proteins are present in cells of Chlamydomonas eugametos (Volvocales).
Using both monoclonal and polyclonal antibodies against mammalian plectin (multifunctional protein cross-linking cytoskeletal structures, mainly intermediate filaments, in mammalian cells), several putative isoforms of plectin-like proteins were found in protein extracts from the green alga Chlamydomonas eugametos (Volvocales). Immunofluorescence and immunoblotting revealed that some of the plectin-like proteins were present in perinuclear region or localized near the cell wall, probably being attached to the cytoplasmic membrane.
Hendrychová
J
J
Division of Autotrophic Microorganisms, Institute of Microbiology, Academy of Sciences of the Czech Republic, 379 81 Trebon.
Vítová
M
M
Bisová
K
K
Wiche
G
G
Zachleder
V
V
D020418:Algal Proteins / Q000378:metabolism*; D000818:Animals; D002696:Chlamydomonas / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D051190:Plectin; D011817:Rabbits; D013347:Subcellular Fractions / Q000378:metabolism
D020418:Algal Proteins; D007381:Intermediate Filament Proteins; D051190:Plectin