[Immune response of mice to replication-defective recombinant adenovirus containing glycoprotein gene of rabies virus 3aG strain].
OBJECTIVE
To evaluate the immune response, of mice immunized with E1, E3 deleted replicative deficient recombinant adenovirus which can express glycoprotein of Chinese 3aG rabies virus.


METHODS
Rapid fluorescent focus inhibition test (RFFIT) was used to determine the titer of neutralizing antibodies; specific antigen induced spleen lymphocyte proliferation was determined by in vitro 3H-TdR in-corporation assay, and lethal intracerebral challenge with CVS rabies virus was used to evaluate the protective effectiveness of the recombinant virus as candidate vaccine.


RESULTS
Mice immunized by 1 x 10(7) recombinant virus developed high levels of rabies virus neutralizing antibodies (VNA). The spleen lymphocyte of the immunized mice also demonstrated remarkable increased proliferation after stimulation with inactivated rabies virus; 70%-90% of the mice immunized by the recombinant virus were protected from lethal intracerebral challenge with CVS rabies virus.


CONCLUSIONS
E1, E3 deleted replication defective recombinant adenovirus containing 3aG glycoprotein gene may be developed as effective rabies vaccine to control rabies in China.
Li
W
W
Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China.
Zhang
Y
Y
Wang
S
S
D000818:Animals; D000917:Antibody Formation; D000956:Antigens, Viral / Q000276:immunology*; D005260:Female; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology; D051379:Mice; D011818:Rabies / Q000276:immunology / Q000517:prevention & control*; D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000235:genetics / Q000276:immunology; D011897:Random Allocation; D013601:T-Lymphocytes / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology
D000956:Antigens, Viral; D006023:Glycoproteins; D011819:Rabies Vaccines; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C079096:adenovirus 5, rabies glycoprotein recombinant vaccine; C064951:glycoprotein G, Rabies virus