[Determination of protocatechuic acid in Blumea riparia (Bl.) DC. by RP-HPLC].
OBJECTIVE
To determine the content of protocatechuic acid in Blumea riparia by RP-HPLC.


METHODS
mu-Bondapak C18 column was used, mobile phase consisted of methanol-water-glacial acetic acid(19:80:1) and detection was performed at UV 260 nm.


RESULTS
The standard curve was linear in the range of 3.31-41.8 micrograms.ml-1. The correlation coefficient was 0.9999. The average recovery rate and RSD were 98.05% and 1.94% (n = 6) respectively.


CONCLUSIONS
The method provides scientific indexes for quality control of riparia.
Xie
P D
PD
Guangxi Provincial Institute for Drug Control, Nanning 530021, Guangxi, China.
Sang
T
T
Gong
X Z
XZ
D019659:Asteraceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D062385:Hydroxybenzoates / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*
D062385:Hydroxybenzoates; C009091:protocatechuic acid