Campylobacter jejuni cytolethal distending toxin promotes DNA repair responses in normal human cells.
Cytolethal distending toxin (CDT) is a multisubunit protein found in various gram-negative bacterial pathogens of humans which is thought to cause cell death by direct DNA damage of host cells. We sought to determine if a cellular response to DNA damage could be detected by exogenous addition of the holotoxin. Exogenous addition of the Campylobacter jejuni 81-176 CDT to primary human fibroblasts resulted in formation of Rad50 foci, which are formed around double-stranded-DNA breaks. Moreover, such foci are formed in both proliferating and nonproliferating cells that are treated with C. jejuni CDT. Fibroblasts that were intoxicated and later stimulated to proliferate failed to divide and remained arrested in the G(1) phase of the cell cycle.
Hassane
Duane C
DC
Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky 40536, USA.
Lee
Robert B
RB
Pickett
Carol L
CL
D017766:Acid Anhydride Hydrolases; D001427:Bacterial Toxins / Q000494:pharmacology*; D016123:Campylobacter jejuni / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004260:DNA Repair / Q000187:drug effects*; D045643:DNA Repair Enzymes; D004268:DNA-Binding Proteins / Q000378:metabolism; D005347:Fibroblasts; D006801:Humans
D001427:Bacterial Toxins; D004268:DNA-Binding Proteins; C067044:cytolethal distending toxin; D017766:Acid Anhydride Hydrolases; C112158:RAD50 protein, human; D045643:DNA Repair Enzymes