title: Na+-K+-ATPase alpha 2-gene and skeletal muscle characteristics in response to long-term overfeeding.
abstract: The role of Na(+)-K(+)-ATPase alpha2-gene BglII polymorphism in the changes of skeletal muscle metabolic properties after a 100-day overfeeding protocol conducted with 12 pairs of monozygotic twins is reported. The activities of oxoglutarate dehydrogenase (OGDH) and phosphofructokinase (PFK) were determined from muscle biopsies. A larger increase in the total fat mass (127 vs. 61%) (P < 0.05) and low-density lipoprotein cholesterol (20 vs. 0.7%) (P = 0.05) in 8.0/8.0-kb [3.3-kb negative (-); n = 7 pairs] than in 8.0/3.3 + 3.3/3.3-kb [3.3-kb positive (+); n = 5 pairs] subjects was observed. OGDH activity decreased in 3.3-kb(-) (-15%), whereas PFK (+26%) as well as the PFK-to-OGDH ratio (90%) increased. In contrast, among 3.3-kb(+), OGDH increased (+54%) together with a decrease in PFK (-1%) and PFK-to-OGDH ratio (-5%). These changes were significantly different between genotypes (P from <0.05 to 0.01). In conclusion, fat mass, low-density lipoprotein cholesterol, and skeletal muscle glycolytic-to-oxidative enzyme ratio increased more in the alpha2-gene 3.3-kb(-) subjects with overfeeding, suggesting more unfavorable metabolic changes compared with the 3.3-kb(+) subjects.
authors.affiliation: Pennington Biomedical Research Center, Baton Rouge, Louisiana 70808-4124, USA.
authors.forename: Olavi
authors.initials: O
authors.lastname: Ukkola
authors.forename: Denis R
authors.initials: DR
authors.lastname: Joanisse
authors.forename: Angelo
authors.initials: A
authors.lastname: Tremblay
authors.forename: Claude
authors.initials: C
authors.lastname: Bouchard
mesh_terms: D000273:Adipose Tissue / Q000502:physiology; D000328:Adult; D001481:Basal Metabolism / Q000502:physiology; D001786:Blood Glucose / Q000378:metabolism; D001823:Body Composition; D005091:Exons / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D007328:Insulin / Q000097:blood; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology*; D009765:Obesity / Q000201:enzymology / Q000235:genetics; D044343:Overnutrition; D011110:Polymorphism, Genetic / Q000235:genetics; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D014430:Twins, Monozygotic
chemical_list: D001786:Blood Glucose; D005819:Genetic Markers; D007328:Insulin; D008055:Lipids; D007655:Ketoglutarate Dehydrogenase Complex; C498531:ATP1A2 protein, human; D000254:Sodium-Potassium-Exchanging ATPase