Supercritical fluid extraction of all-trans-lycopene from tomato.
A procedure is proposed for the supercritical fluid extraction of all-trans-lycopene from tomato using carbon dioxide at 40 degrees C without modifier. The present method minimizes the risk of degradation via isomerization and oxidation of health-promoting ingredients, such as lycopene. The effect of different experimental variables on the solvating power of the supercritical fluid was evaluated in terms of both the selectivity achievable in the process and the yield of the extraction of all-trans-lycopene. Satisfactory separations of the all-trans-lycopene isomers from the cis counterparts were achieved using a C(30) column. The obtained extract contained 88% all-trans-lycopene and 12% cis-lycopene.
Gómez-Prieto
M Salud
MS
Instituto de Fermentaciones Industriales, CSIC. c/ Juan de la Cierva 3, 28006 Madrid, Spain.
Caja
M Mar
MM
Herraiz
Marta
M
Santa-María
Guillermo
G
D002338:Carotenoids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D005638:Fruit / Q000737:chemistry; D007536:Isomerism; D000077276:Lycopene; D018551:Solanum lycopersicum / Q000737:chemistry*
D002338:Carotenoids; D000077276:Lycopene