Subcritical water extraction of antioxidant compounds from rosemary plants.
Subcritical water extraction at several temperatures ranging from 25 to 200 degrees C has been studied to selectively extract antioxidant compounds from rosemary leaves. An exhaustive characterization of the fractions obtained using subcritical water at different temperatures has been carried out by LC-MS, and the antioxidant activities of the extracts have been measured by a free radical method (DPPH). Results indicate high selectivity of the subcritical water toward the most active compounds of rosemary such as carnosol, rosmanol, carnosic acid, methyl carnosate, and some flavonoids such as cirsimaritin and genkwanin. The antioxidant activity of the fractions obtained by extraction at different water temperatures was very high, with values around 11.3 microg/mL, comparable to those achieved by SFE of rosemary leaves. A study of the effect of the temperature on the extraction efficiency of the most typical rosemary antioxidant compounds has been performed.
Ibañez
Elena
E
Instituto de Fermentaciones Industriales, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain. elena@ifi.csic.es
Kubátová
Alena
A
Señoráns
F Javier
FJ
Cavero
Sofia
S
Reglero
Guillermo
G
Hawthorne
Steven B
SB
D045784:Abietanes; D000975:Antioxidants / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004224:Diterpenes / Q000302:isolation & purification; D005419:Flavonoids / Q000302:isolation & purification; D013058:Mass Spectrometry; D015394:Molecular Structure; D010616:Phenanthrenes / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry*; D027542:Rosmarinus / Q000737:chemistry*; D013696:Temperature; D014867:Water
D045784:Abietanes; D000975:Antioxidants; D004224:Diterpenes; D005419:Flavonoids; D010616:Phenanthrenes; D010936:Plant Extracts; D014867:Water; C068623:carnosol; C465581:rosmanol; C018381:salvin