title: Cloning and characterization of a novel chromosomal drug efflux gene in Staphylococcus aureus.
abstract: A novel drug efflux gene (named sepA, staphylococcal efflux pump gene) is cloned from antiseptic-resistant mutants of Staphylococcus aureus into Escherichia coli. The sepA gene conferred the reduction of susceptibility to acriflavine and the acceleration of ethidium bromide efflux from the E. coli cells. The sepA (474 bp) encoded the protein that has four predicted transmembrane segments. These results indicate that sepA gene is a multidrug-resistant gene and encodes a drug efflux protein.
authors.affiliation: Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Narui
authors.forename: Norihisa
authors.initials: N
authors.lastname: Noguchi
authors.forename: Kazunori
authors.initials: K
authors.lastname: Wakasugi
authors.forename: Masanori
authors.initials: M
authors.lastname: Sasatsu
mesh_terms: D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D001426:Bacterial Proteins; D002876:Chromosomes, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D019450:Genes, MDR / Q000187:drug effects / Q000235:genetics*; D008666:Metalloendopeptidases / Q000235:genetics*; D008826:Microbial Sensitivity Tests / Q000706:statistics & numerical data; D008969:Molecular Sequence Data; D009154:Mutation; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000891:Anti-Infective Agents, Local; D001426:Bacterial Proteins; D008666:Metalloendopeptidases; C081903:SepA protein, Staphylococcus epidermidis