[Determination of nodakenin in Peucedanum].
OBJECTIVE
To establish a HPLC method to determine the contents of nodakenin in Peucedanum.


METHODS
The determination method was achieved by RP-HPLC on an ODS column by UV detection at 334 nm; with nodakenin as external standard and H2O-CH3OH-CH3CN(75:5:20) as mobile phase.


RESULTS
Several samples of 6 species of Peucedanum were analyzed by this method. The contents of nodakenin ranged from 0.005%-3.090%.


CONCLUSIONS
Nodakenin cannot be used to control the quality of Peucedanum for pharmaceutical use, but this method is a simple and feasible way to control the quality of P. decursivum.
Xu
Q
Q
Department of Pharmaceutical Analysis, Guilin Medical College, Guilin 541004, Guangxi, China.
Liu
B M
BM
D019661:Apiaceae / Q000737:chemistry* / Q000145:classification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003374:Coumarins; D005960:Glucosides / Q000032:analysis; D006575:Heterocyclic Compounds, 3-Ring / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
D003374:Coumarins; D005960:Glucosides; D006575:Heterocyclic Compounds, 3-Ring; C471579:nodakenin