Specific modulation of the anti-DNA autoantibody-nucleic acids interaction by the high affinity RNA aptamer.
Anti-DNA autoantibodies are one of the frequently found autoantibodies in systemic lupus erythematosus patient sera. RNA aptamers for the monoclonal G6-9 anti-DNA autoantibody were selected from a random pool of RNA library. Binding affinity of the best aptamer is around 2nM, which is at least 100-fold higher than that of cognate DNA antigen to the autoantibody. Aptamer binds specifically to the G6-9 autoantibody but not to other similar autoantibodies. Minimal binding motif of the aptamer was mapped, providing a hint for a natural epitope of the autoantibody. DNA binding to the G6-9 autoantibody is shown to be efficiently inhibited by the aptamer. Such binding property of the RNA aptamer could be used not only as a modulator for the pathogenic anti-DNA autoantibody, but also as a useful biochemical reagent for elucidating a fine specificity of the autoantibody-nucleic acid interaction.
Kim
Young-Mee
YM
Department of Molecular Biology, College of Natural Sciences, Dankook University, Hannam-dong san 8, Yongsan-ku, Seoul 140-714, Republic of Korea.
Choi
Kang Hyun
KH
Jang
Young-Ju
YJ
Yu
Jaehoon
J
Jeong
Sunjoo
S
D000974:Antibodies, Antinuclear / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D004247:DNA / Q000276:immunology*; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D020349:Surface Plasmon Resonance
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D009843:Oligoribonucleotides; D012313:RNA; D004247:DNA