title: Specific modulation of the anti-DNA autoantibody-nucleic acids interaction by the high affinity RNA aptamer.
abstract: Anti-DNA autoantibodies are one of the frequently found autoantibodies in systemic lupus erythematosus patient sera. RNA aptamers for the monoclonal G6-9 anti-DNA autoantibody were selected from a random pool of RNA library. Binding affinity of the best aptamer is around 2nM, which is at least 100-fold higher than that of cognate DNA antigen to the autoantibody. Aptamer binds specifically to the G6-9 autoantibody but not to other similar autoantibodies. Minimal binding motif of the aptamer was mapped, providing a hint for a natural epitope of the autoantibody. DNA binding to the G6-9 autoantibody is shown to be efficiently inhibited by the aptamer. Such binding property of the RNA aptamer could be used not only as a modulator for the pathogenic anti-DNA autoantibody, but also as a useful biochemical reagent for elucidating a fine specificity of the autoantibody-nucleic acid interaction.
authors.affiliation: Department of Molecular Biology, College of Natural Sciences, Dankook University, Hannam-dong san 8, Yongsan-ku, Seoul 140-714, Republic of Korea.
authors.forename: Young-Mee
authors.initials: YM
authors.lastname: Kim
authors.forename: Kang Hyun
authors.initials: KH
authors.lastname: Choi
authors.forename: Young-Ju
authors.initials: YJ
authors.lastname: Jang
authors.forename: Jaehoon
authors.initials: J
authors.lastname: Yu
authors.forename: Sunjoo
authors.initials: S
authors.lastname: Jeong
mesh_terms: D000974:Antibodies, Antinuclear / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D004247:DNA / Q000276:immunology*; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D020349:Surface Plasmon Resonance
chemical_list: D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D009843:Oligoribonucleotides; D012313:RNA; D004247:DNA