[Biological analysis of a novel gene related with interleukin-6].
OBJECTIVE
To understand the action mechanism of interleukin (IL)-6 through investigation of its related genes.


METHODS
Using electronic cloning with IL-6-related expressed sequence tags (ESTs), a novel gene at its full length of 924 bp was acquired. Reverse transcriptase-PCR method was subsequently employed to amplify the cDNA of this gene from the total RNA of human U937 cells, which were previously activated with 100 ng/ml IL-6 for 8 h. The fragment was then ligated into the pGEM-Teasy vector and sequence analysis was conducted.


RESULTS
The whole cDNA gene was successfully fished.


CONCLUSIONS
Electronic cloning technology can be instrumental in identifying novel genes.
Xia
Rong
R
Deparment of Transfusion, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Li
Yan
Y
Lin
Lun-jiang
LJ
Feng
Jian-nan
JN
Lan
Jiong-cai
JC
Shen
Bei-fen
BF
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification*; D020224:Expressed Sequence Tags; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020298:U937 Cells
D018076:DNA, Complementary; D015850:Interleukin-6