Cell microarrays: an emerging technology for the characterization of antibodies.
The possibility to miniaturize and parallelize biological assays has a great impact on the development of biomedical technologies. Here, we describe a simple, miniaturized, and parallelized method employing entire cells from different cell lines displaying a protein of interest on their surface, which were immobilized on a microarray slide. Antibodies were added to these cellular microarrays, and their specific binding to the cell surface proteins was monitored using appropriate fluorescently labeled detection molecules. This new method is applicable for rapidly screening cell surface-specific antibodies with respect to selectivity and cross-reactivity.
Schwenk
Jochen M
JM
NMI-Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany.
Stoll
Dieter
D
Templin
Markus F
MF
Joos
Thomas O
TO
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry*; D018914:Cells, Immobilized / Q000737:chemistry; D004867:Equipment Design; D006801:Humans; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry*; D008567:Membranes, Artificial; D051379:Mice; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D011485:Protein Binding
D000906:Antibodies; D046911:Macromolecular Substances; D008565:Membrane Proteins; D008567:Membranes, Artificial