[Study on the relationship between cadmium immunotoxicity and corticotropin-releasing factor].
To explore the relationship between cadmium immunotoxicity and corticotropin-releasing factor(CRF), murine T- and B-lymphocytes mitogen-induced proliferation function(3H-TdR incorporation method) and CRF levels of hypothalami and blood plasma were observed both in cadmium chloride(CdCl2) and CdCl2 + alpha-hCRF-treated animals in vivo. In cadmium-treated animals, T and B lymphocytes stimulation indexes were significantly lower than those in control(P < 0.05) with a dose dependent response. And interestingly, the central CRF levels in CdCl2-treated groups were significantly higher than those in control (P < 0.05), an increasing CRF level was also observed accompanied by the increase of CdCl2 level. In peripheral blood plasma, no significant difference of CRF levels was found among all groups(P > 0.05). When animals were treated with CdCl2 in combination with alpha-hCRF, no significant difference of B-lymphocyte proliferation function existed among the alpha-hCRF + CdCl2 groups (P > 0.05). Similar results were also observed in detecting T-lymphocyte proliferation function. Hypothalamic CRF levels among various doses of CdCl2 + alpha-hCRF groups showed no statistical difference, but all were higher than those in the control, and peripheral CRF levels had a trend to increase in alpha-hCRF + CdCl2 co-administrated groups. The results suggested that CdCl2 showed an immunosuppressive action upon murine T and B lymphocytes, CdCl2 could cause central CRF increase. alpha-hCRF could partially reduce the cadmium immunotoxicity. Central CRF-mediated immunomodulation was shown in cadmium immunotoxical effects on murine splenic lymphocytes.
Zhang
X
X
Department of Environment Health, Sun Yat-Sen University of Medical Science, Guangzhou 510089, China.
Li
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D
Dong
S
S
Zhang
J
J
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D019256:Cadmium Chloride / Q000633:toxicity*; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism* / Q000633:toxicity; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*
C069206:alpha helical corticotropin-releasing hormone; D003346:Corticotropin-Releasing Hormone; D019256:Cadmium Chloride