Development of an AFLP-based linkage map and localization of QTLs for seed fatty acid content in condiment mustard (Brassica juncea).
A genetic linkage map of Brassica juncea based on AFLP and RAPD markers was constructed using 131 F1-derived doubled-haploid (DH) plants from a cross between two mustard lines. The map included 273 markers (264 AFLP, 9 RAPD) arranged on 18 linkage groups, and covered a total genetic distance of 1641 cM; 18.3% of the AFLP markers showed a segregation distortion (P < 0.01). The markers with biased segregation were clustered on seven linkage groups. QTLs for oil contents, palmitic acid (16:0), stearic acid (18:0), oleic acid (18:1), linoleic acid (18:2), linolenic acid (18:3), eicosenoic acid (20:1), and erucic acid (22:1), were mapped on the AFLP linkage map. Correlation studies among fatty acids in the DH population and the localization of QTLs involved in their control indicated that a major gene located on linkage group (LG) 2 controlled the elongation step of erucic acid.
Lionneton
E
E
Institut National de la Recherche Agronomique (INRA), Unité de Recherche en Génétique et Amélioration des Plantes (URGAP), BP 86510, 21065 Dijon cedex, France. eric.lionneton@epoisses.inra.fr
Ravera
S
S
Sanchez
L
L
Aubert
G
G
Delourme
R
R
Ochatt
S
S
D001937:Brassica / Q000196:embryology / Q000235:genetics*; D005227:Fatty Acids / Q000032:analysis*; D008040:Genetic Linkage; D018745:Genome, Plant; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci; D012639:Seeds / Q000737:chemistry*
D005227:Fatty Acids