A new experimental model in rats for study of the pathophysiology of subarachnoid hemorrhage.
A new experimental model of subarachnoid hemorrhage (SAH) in rats is described. A needle was stereotaxically placed in the prechiasmatic cistern and 300, 250 or 200 microl of blood was injected manually, keeping the intracranial pressure (ICP) at the mean arterial blood pressure (MABP) level. An acceptable mortality was observed only after injection of 200 microl of blood. In this group, MABP and ICP increased immediately after SAH, but soon approached baseline levels. The subarachnoid blood was mainly distributed in the basal cisternal system and its estimated volume was about 95% of the amount injected. This new model resembles clinical SAH, is very reproducible, easy to use and seems to be a suitable model for studies of the pathophysiology of SAH.
Prunell
Giselle F
GF
Department of Clinical Neuroscience, Section for Neurosurgery, Karolinska Institutet, Box 130, 171 76 Stockholm, Sweden. giselle.prunell@ks.se
Mathiesen
Tiit
T
Svendgaard
Niels-Aage
NA
D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013345:Subarachnoid Hemorrhage / Q000401:mortality / Q000503:physiopathology*; D014951:Wounds, Stab / Q000401:mortality / Q000503:physiopathology