[Comparison of infectious brain edema models induced by different kinds of pertussis bacilli in rats].
OBJECTIVE
To understand the difference of infectious brain edema models induced by varied kinds of pertussis bacilli in rats and to look for a new infectious brain edema model.


METHODS
Sprague-Dawley rats were randomly divided into 3 groups: normal saline group(NS); rude pertussis bacilli(PBR); purified pertussis bacilli(PBP). After infectious brain edema model was induced by rude pertussis bacilli or purified pertussis bacilli, the water content(WC), Evan's blue content(EB), Na+ and K+ content in brain tissues were measured.


RESULTS
The WC, EB and Na+ contents in brain tissues in PBR and PBP groups were significantly increased than those in NS group; K+ content was significantly decreased in PBR and PBP groups than that in NS group(P < 0.01). Evan's blue discoloration was demonstrated in all rats of PBR group, but 6 rats of PBP group showed bluish discoloration. EB content in brain tissues was significantly increased in PBR group than that in PBP group.


CONCLUSIONS
The infectious brain edema model can be induced by rude pertussis bacilli or purified pertussis bacilli in rats, the former gives priority to mixed brain edema, and the latter mainly avails cytotoxin brain edema.
Yin
F
F
Department of Pediatrics, Xiangya Hospital, Hunan Medical University, Changsha 410008.
Yang
Y J
YJ
Liu
C Q
CQ
D000818:Animals; D001886:Bordetella pertussis; D001929:Brain Edema / Q000382:microbiology*; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014917:Whooping Cough