title: Evaluation of MicroScan Autoscan for identification of Pseudomonas aeruginosa isolates from cystic fibrosis patients.
abstract: Accurate identification of gram-negative bacilli from cystic fibrosis (CF) patients is essential. Only 57% (108 of 189) of nonmucoid strains and 40% (24 of 60) of mucoid strains were definitively identified as Pseudomonas aeruginosa with MicroScan Autoscan. Most common misidentifications were Pseudomonas fluorescens-Pseudomonas putida (i.e., the strain was either P. fluorescens or P. putida, but the system did not make the distinction and yielded the result P. fluorescens/putida) and Alcaligenes spp. Extending the incubation to 48 h improved identification, but 15% of isolates remained misidentified. The MicroScan Autoscan system cannot be recommended for the identification of P. aeruginosa isolates from CF patients.
authors.affiliation: Department of Pediatrics, Division of Infectious Diseases, Columbia University, New York, New York 10032, USA. ls5@columbia.edu
authors.forename: Lisa
authors.initials: L
authors.lastname: Saiman
authors.forename: Jane L
authors.initials: JL
authors.lastname: Burns
authors.forename: Davise
authors.initials: D
authors.lastname: Larone
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Chen
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Garber
authors.forename: Susan
authors.initials: S
authors.lastname: Whittier
mesh_terms: D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic
chemical_list: D011933:Reagent Kits, Diagnostic