Seeligeriolysin O, a cholesterol-dependent cytolysin of Listeria seeligeri, induces gamma interferon from spleen cells of mice.
Seeligeriolysin O (LSO), one of the cholesterol-dependent cytolysins produced by Listeria seeligeri, shows 80% homology to listeriolysin O (LLO) produced by Listeria monocytogenes at the amino acid sequence level. In addition to cytolytic activity, LLO has been shown to exhibit cytokine-inducing activity. In order to determine whether LSO is also capable of exhibiting these two different activities, we constructed a recombinant full-length LSO (rLSO530) and a noncytolytic truncated derivative with a C-terminal deletion (rLSO483) and compared these molecules with recombinant LLO. The cytolytic rLSO530 molecule could induce gamma interferon (IFN-gamma) production in spleen cells when the cytolytic activity was blocked by treatment with cholesterol. The noncytolytic truncated rLSO483 molecule also induced IFN-gamma production. Anti-LLO polyclonal antibody inhibited not only LLO-induced IFN-gamma production but also LSO-induced IFN-gamma production. Both NK cells and CD11b(+) cells were required for LSO-induced IFN-gamma production. Among the various cytokines expressed in CD11b(+) cells, interleukin-12 (IL-12) and IL-18 appeared to be essential. We concluded that LSO exhibits the same biological activity as LLO.
Ito
Yutaka
Y
Department of Microbiology, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan.
Kawamura
Ikuo
I
Kohda
Chikara
C
Baba
Hisashi
H
Nomura
Takamasa
T
Kimoto
Terumi
T
Watanabe
Isao
I
Mitsuyama
Masao
M
D000818:Animals; D001427:Bacterial Toxins; D039481:CD11b Antigen / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D003603:Cytotoxins / Q000276:immunology*; D005260:Female; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D007371:Interferon-gamma / Q000096:biosynthesis*; D008087:Listeria / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D011994:Recombinant Proteins / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology*
D001427:Bacterial Toxins; D039481:CD11b Antigen; D016207:Cytokines; D003603:Cytotoxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins; D007371:Interferon-gamma; D002784:Cholesterol; C038705:hlyA protein, Listeria monocytogenes