title: Persistent Sin Nombre virus infection in the deer mouse (Peromyscus maniculatus) model: sites of replication and strand-specific expression.
abstract: To address Sin Nombre (SN) virus persistence in deer mice, we sacrificed experimentally infected deer mice at eight time points from day 21 to day 217 postinoculation (p.i.) and examined their tissues for viral nucleocapsid (N) antigen expression and both negative-strand (genomic) and positive-strand (replicative/mRNA) viral S segment RNA titers. All the animals that we inoculated developed persistent infections, and SN virus could be isolated from tissues throughout the course of infection. The transition from an acute to a persistent pattern of infection appeared to occur between days 60 and 90 p.i. Beginning on day 60 p.i., the heart, brown adipose tissue (BAT), and lung retained antigen expression and genomic viral RNA the most frequently. We found a statistically significant association among the presence of replicative RNA in the heart, lung, and BAT, widespread antigen expression (in > or =5 tissues), and RNA viremia. Of these three tissues, the heart retained negative-strand RNA and viral N antigen the most consistently (in 25 of 26 animals). During persistence, there were two distinct patterns of infection: restricted versus disseminated tissue involvement. Mice with the restricted pattern exhibited N antigen expression in < or =3 tissues, an absence of viral RNA in the blood, neutralizing antibody titers of < or =1:1,280 (P = 0.01), and no replicative RNA in the heart, lung, or BAT. Those with the "disseminated" pattern showed N antigen expression in > or =5 tissues, neutralizing antibody titers of 1:160 to 1:20,480, replicative RNA in the heart, lung, and BAT at a high frequency, and RNA viremia. Virus could be isolated consistently only from mice that demonstrated the disseminated pattern. The heart, lung, and BAT are important sites for the replication and maintenance of SN virus during persistent infection.
authors.affiliation: Infectious Diseases and Inflammation Program, University of New Mexico, Albuquerque 87131, USA.
authors.forename: Jason
authors.initials: J
authors.lastname: Botten
authors.forename: Katy
authors.initials: K
authors.lastname: Mirowsky
authors.forename: Donna
authors.initials: D
authors.lastname: Kusewitt
authors.forename: Chunyan
authors.initials: C
authors.lastname: Ye
authors.forename: Keith
authors.initials: K
authors.lastname: Gottlieb
authors.forename: Joseph
authors.initials: J
authors.lastname: Prescott
authors.forename: Brian
authors.initials: B
authors.lastname: Hjelle
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D018804:Hantavirus Pulmonary Syndrome / Q000821:virology*; D009500:Neutralization Tests; D010542:Peromyscus; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029261:Sin Nombre virus / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D019562:Viral Load; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral