Human T lymphocyte response to Borrelia burgdorferi infection: no correlation between human leukocyte function antigen type 1 peptide response and clinical status.
We tested the hypothesis that cross-reactivity between the outer surface protein A (OspA) of Borellia burgdorferi and human leukocyte function antigen (LFA) type 1 mediates chronic autoimmune sequelae of Lyme disease. T cell response was studied in subjects with Lyme disease presenting with erythema migrans alone (n=36), erythema migrans with neurological disease (n=12), and chronic Lyme disease syndrome (n=20), as well as healthy control subjects from Lyme-endemic (n=50) and -nonendemic (n=18) regions. Antigens included recombinant OspA and OspC (all strain B31) and human LFA-1 peptide (IYVIEGTSKQDLTSF). Proliferation to OspA was detected in 11 (28%) of 39 of subjects presenting with erythema migrans, which increased to 50% at 4 weeks of follow-up. Reactivity to OspA and LFA-1 was significantly correlated (P<.001) and was observed in 18 (78%) of 23 of OspA-responsive subjects. However, there was no correlation between T cell response to human LFA-1 peptide and clinical status.
Kalish
Richard S
RS
Department of Dermatology, State University of New York at Stony Brook, 11794-8165, USA. richard.kalish@stonybrook.edu
Wood
Jonathan A
JA
Golde
William
W
Bernard
Robert
R
Davis
Larry E
LE
Grimson
Roger C
RC
Coyle
Patricia K
PK
Luft
Benjamin J
BJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial; D000954:Antigens, Surface / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001428:Bacterial Vaccines; D003429:Cross Reactions; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D008074:Lipoproteins; D008193:Lyme Disease / Q000276:immunology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology*; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D008074:Lipoproteins; D016169:Lymphocyte Function-Associated Antigen-1; C072045:OspA protein; C079616:OspC protein; D007371:Interferon-gamma