[Investigation on antibacterial activity of Forsythia suspense Vahl in vitro with Mueller-Hinton agar].
OBJECTIVE
To evaluate the antibacterial activity of Forsythia suspensa in vitro with different media.


METHODS
MIC determination of Forsythia suspensa against Staphylococci was performed by the agar dilution method.


RESULTS
MIC90 of decoction of Forsythia suspensa against Staphylococcus epidermidis in M-H agar was 1:640, but in nutrient agar 1:40, the antibacterial activity with M-H agar being 16 fold higher than nutrient agar.


CONCLUSIONS
The M-H agar should be recommended to replace nutrient agar as medium in the antibacterial experiment of Traditional Chinese medicine, and it is better to use multipoint inoculating device in the sensitivity test.
Li
Z X
ZX
Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, China.
Wang
X H
XH
Zhao
J H
JH
Yang
J F
JF
Wang
X
X
D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D031657:Forsythia; D008826:Microbial Sensitivity Tests; D010946:Plants, Medicinal; D013211:Staphylococcus aureus / Q000187:drug effects*; D013212:Staphylococcus epidermidis / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D003470:Culture Media; D004365:Drugs, Chinese Herbal; D000362:Agar