Spontaneous establishment of a novel Japanese macaque cell line with epithelial cell phenotypes.
A novel macaque cell line (J3K) with epithelial phenotypes was spontaneously established from a kidney specimen of a Japanese macaque (Macaca fuscata). Its population doubling level reached more than 500, and it was regarded as an established permanent cell line. J3K cells have transformed cell phenotypes such as loss of contact inhibition and anchorage-independent cell growth. Unlike other monkey adherent cell lines, J3K had no SV40 large T antigen. After establishment, cells have constantly expressed telomerase activity, whereas telomere length has been maintained. No mutations in the coding regions of the p53 complementary deoxyribonucleic acid were detected in the late-passaged cells. J3K, a novel transformed epithelial cell line, will be useful material for the comparative study of human and other primate cellular aging as well as cancer cell biology.
Shimizu
Yuko
Y
Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Ishida
Takafumi
T
D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004847:Epithelial Cells / Q000201:enzymology / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D007564:Japan; D008252:Macaca fascicularis; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010641:Phenotype; D019098:Telomerase / Q000378:metabolism; D016615:Telomere; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D016159:Tumor Suppressor Protein p53; D019098:Telomerase