Spectral response for laser enhancement in hepatic regeneration for hepatectomized rats.
OBJECTIVE
The low intensity laser therapy (LILT) has been widely used in all medical fields due to its therapeutic effects in reparative process, pain relief, and biostimulation. Even though there is a therapeutic window of wavelengths for clinical application, little has been done concerning the frequency spectrum response to biological effects. In this work, we investigate the dependence of different wavelengths irradiation in the enhancement of the tissue regeneration after partial hepatectomy in Wistar rats.


METHODS
The proliferating cell nuclear antigen (PCNA) labeling index and the respiratory control (oxygen consumption in extracted mitochondria) were the tests used to evaluate the liver regeneration after laser irradiation with different wavelengths.


CONCLUSIONS
The results show a correlated spectral response that can be explained based on the combined effect of light penetration on biological tissues and the biomolecular excitation efficiency for each wavelength used.
Castro-e-Silva
O
O
Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. dos Bandeirantes 3900, 14048-900 Ribeirão Preto, São Paulo, Brazil.
Zucoloto
S
S
Marcassa
L G
LG
Marcassa
J
J
Kurachi
C
C
Melo
C A S
CA
Ramalho
F S
FS
Ramalho
L N Z
LN
Bagnato
V S
VS
D000818:Animals; D004195:Disease Models, Animal; D004307:Dose-Response Relationship, Radiation; D006498:Hepatectomy; D008107:Liver Diseases / Q000532:radiotherapy* / Q000601:surgery*; D008115:Liver Regeneration / Q000528:radiation effects*; D028022:Low-Level Light Therapy; D008297:Male; D008930:Mitochondria, Liver / Q000528:radiation effects; D010101:Oxygen Consumption / Q000528:radiation effects; D018809:Proliferating Cell Nuclear Antigen / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D013057:Spectrum Analysis; D013997:Time Factors
D018809:Proliferating Cell Nuclear Antigen