title: Quaternary structure of hemoglobin in solution.
abstract: Many important proteins perform their physiological functions under allosteric control, whereby the binding of a ligand at a specific site influences the binding affinity at a different site. Allosteric regulation usually involves a switch in protein conformation upon ligand binding. The energies of the corresponding structures are comparable, and, therefore, the possibility that a structure determined by x-ray diffraction in the crystalline state is influenced by its intermolecular contacts, and thus differs from the solution structure, cannot be excluded. Here, we demonstrate that the quaternary structure of tetrameric human normal adult carbonmonoxy-hemoglobin can readily be determined in solution at near-physiological conditions of pH, ionic strength, and temperature by NMR measurement of (15)N-(1)H residual dipolar couplings in weakly oriented samples. The structure is found to be a dynamic intermediate between two previously solved crystal structures, known as the R and R2 states. Exchange broadening at the subunit interface points to a rapid equilibrium between different structures that presumably include the crystallographically observed states.
authors.affiliation: Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA.
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Lukin
authors.forename: Georg
authors.initials: G
authors.lastname: Kontaxis
authors.forename: Virgil
authors.initials: V
authors.lastname: Simplaceanu
authors.forename: Yue
authors.initials: Y
authors.lastname: Yuan
authors.forename: Ad
authors.initials: A
authors.lastname: Bax
authors.forename: Chien
authors.initials: C
authors.lastname: Ho
mesh_terms: D002263:Carboxyhemoglobin / Q000737:chemistry; D003460:Crystallization; D019281:Dimerization; D006441:Hemoglobin A / Q000737:chemistry*; D006801:Humans; D020836:Protein Structure, Quaternary; D012996:Solutions
chemical_list: D012996:Solutions; D006441:Hemoglobin A; D002263:Carboxyhemoglobin