title: Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis.
abstract: Serology (ELISA and immunoblot) using native glycoproteins, affinity purified glycoproteins, and a recombinant antigen is known to be highly specific to Taenia solium cysticercosis in humans and pigs. These techniques were applied for dogs in the highly endemic area of cysticercosis in Papua (Irian Jaya), Indonesia. Analysis of dog sera by both ELISA and immunoblot revealed 7 of 64 dogs were highly positive. Examination of two sero-positive dogs revealed cysticerci of T. solium in the brain and heart of these dogs. Mitochondrial DNA analysis confirmed that they were the same as T. solium previously confirmed from pigs and biopsies from local people from Irian Jaya. It is suggested that the life cycle of T. solium may be completed not only between humans and pigs but also between humans and dogs.
authors.affiliation: Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan. akiraito@asahikawa-med.ac.jp
authors.forename: A
authors.initials: A
authors.lastname: Ito
authors.forename: M I
authors.initials: MI
authors.lastname: Putra
authors.forename: R
authors.initials: R
authors.lastname: Subahar
authors.forename: M O
authors.initials: MO
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Okamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Sako
authors.forename: M
authors.initials: M
authors.lastname: Nakao
authors.forename: H
authors.initials: H
authors.lastname: Yamasaki
authors.forename: K
authors.initials: K
authors.lastname: Nakaya
authors.forename: P S
authors.initials: PS
authors.lastname: Craig
authors.forename: S S
authors.initials: SS
authors.lastname: Margono
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D003551:Cysticercosis / Q000175:diagnosis / Q000662:veterinary*; D018509:DNA, Helminth / Q000032:analysis; D004272:DNA, Mitochondrial / Q000032:analysis; D004199:Disease Vectors; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D011994:Recombinant Proteins / Q000276:immunology; D013552:Swine / Q000469:parasitology; D013621:Taenia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000947:Antigens, Helminth; D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D011994:Recombinant Proteins