Antigen detection assay for identification of Penicillium marneffei infection.
Two recently produced monoclonal antibodies were used to develop an antigen capture enzyme-linked immunosorbent assay (ELISA) for rapid diagnosis of Penicillium marneffei. The method was evaluated with 53 patients with culture-confirmed penicilliosis and 240 controls. The diagnostic sensitivity, specificity, and accuracy of the ELISA were 92.45, 97.5, and 96.59%, respectively.
Chaiyaroj
Sansanee C
SC
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand. scscy@mahidol.ac.th
Chawengkirttikul
Runglawan
R
Sirisinha
Stitaya
S
Watkins
Pramuan
P
Srinoulprasert
Yuttana
Y
D000911:Antibodies, Monoclonal / Q000276:immunology; D000946:Antigens, Fungal / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009181:Mycoses / Q000382:microbiology*; D010407:Penicillium / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D011933:Reagent Kits, Diagnostic