Ultrastructural study of glutamate- and GABA-immunoreactive terminals contacting the primary afferent fibers in frog spinal cord. A double postembedding immunocytochemical study.
The in vitro HRP application to the dorsal root of the frog spinal cord produced an intensive staining of primary afferent fibers. A double postembedding GABA and glutamate immunocytochemical study revealed GABA- or glutamate-immunopositive presynaptic boutons establishing axo-axonic synapses onto HRP-stained primary afferent fibers in the spinal cord intermedial zone.
Vesselkin
N P
NP
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St-Petersburg, Russia.
Adanina
V O
VO
Rio
J P
JP
Repérant
J
J
D000818:Animals; D005260:Female; D018698:Glutamic Acid / Q000502:physiology*; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D008297:Male; D009475:Neurons, Afferent / Q000648:ultrastructure*; D017729:Presynaptic Terminals / Q000648:ultrastructure*; D011896:Rana temporaria; D013116:Spinal Cord / Q000648:ultrastructure*; D016610:Tissue Embedding; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D006735:Horseradish Peroxidase