Development of 47 new microsatellite markers from a BTA6 library.
Chromosome-specific libraries provide a means to isolate genetic markers from specific chromosomal regions. A small-insert BTA6 library, constructed by microdissection, was screened for dinucleotide repeats (CA)15 and (GA)15. A total of 47 new microsatellite loci were developed and tested for polymorphism and informativeness using the MARC bovine mapping family.
Reed
K M
KM
Department of Veterinary PathoBiology, University of Minnesota, St. Paul, MN, USA.
Ihara
N
N
Ponce de León
F A
FA
Sonstegard
T S
TS
Smith
T P L
TP
Bennett
G L
GL
Sugimoto
Y
Y
Beattie
C W
CW
D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping / Q000662:veterinary; D033481:Chromosomes, Mammalian / Q000235:genetics*; D015723:Gene Library; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000235:genetics