Immunohistochemical, molecular, and pathomorphological study of liver biopsy specimens during chronic hepatitis C.
We carried out a comparative study of replication markers of hepatitis C virus (HCV RNA) in some biological substrates and NS3-antigen in liver biopsy specimens. It was found that 82% liver specimens contained RNA HCV, in 44% cases HCV RNA was present in the serum, mononuclear blood cells, and liver. The presence of NS3-antigen in hepatocytes can be considered as a structural marker of HCV replication, which is confirmed by positive correlation with the results of PCR for viral RNA in tissue specimens. In most cases the presence of HCV replication markers did not correlate with activity of the infectious process assessed by biochemical and histological tests.
Nepomnyashchikh
G I
GI
Laboratory of Ultrastructure Basis of Pathology, Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk. pathol@cyber.ma.nsc.ru
Aidagulova
S V
SV
Nepomnyashchikh
D L
DL
Tolokonskaya
N P
NP
Karavaeva
Yu Yu
YY
Sakharova
E G
EG
Mezentseva
G A
GA
Batemirova
E V
EV
D000293:Adolescent; D000328:Adult; D001706:Biopsy; D002908:Chronic Disease; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000473:pathology* / Q000821:virology*; D022781:Hepatocytes / Q000378:metabolism / Q000821:virology; D006801:Humans; D007150:Immunohistochemistry; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis
D004279:DNA, Viral; D012367:RNA, Viral