title: Inhibitory effects of the root cortex of Paeonia suffruticosa on interleukin-8 and macrophage chemoattractant protein-1 secretions in U937 cells.
abstract: In an effort to elucidate the mechanism of the anti-inflammatory effect of mudanpi, the root cortex of Paeonia suffruticosa Andrews (Ranunculaceae), we determined the effects of the methanolic extract of mudanpi (MEM) on the secretions of interleukin (IL)-8, a major mediator of acute neutrophil-mediated inflammation, and macrophage chemoattractant protein (MCP)-1, a major mediator of chronic macrophage-mediated inflammation, in human monocytic U937 cells stimulated with phorbol myristate acetate (PMA). MEM significantly inhibited PMA-induced secretions of IL-8 and MCP-1 proteins in a dose-dependent manner. The inhibition of these chemokines by MEM was due to its suppression of IL-8 and MCP-1 genes. In addition, 1,2,3,4,6-penta-O-galloyl-beta-D-glucose, one of major constituents isolated from MEM, inhibited PMA-induced secretions of IL-8 and MCP-1 proteins by its suppression of IL-8 and MCP-1 genes. Thus, one possible anti-inflammatory mechanism of mudanpi, an anti-inflammatory Chinese crude drug, may be to inhibit the secretions of inflammatory chemokines.
authors.affiliation: Department of Microbiology and Immunology, Medicinal Resources Research Center of Wonkwang University, Wonkwang University School of Medicine, 344-2 Shinyong-Dong, Iksan-Shi, Chonbuk 570-749, South Korea.
authors.forename: G S
authors.initials: GS
authors.lastname: Oh
authors.forename: H O
authors.initials: HO
authors.lastname: Pae
authors.forename: B M
authors.initials: BM
authors.lastname: Choi
authors.forename: S
authors.initials: S
authors.lastname: Jeong
authors.forename: H
authors.initials: H
authors.lastname: Oh
authors.forename: C S
authors.initials: CS
authors.lastname: Oh
authors.forename: Y D
authors.initials: YD
authors.lastname: Rho
authors.forename: D H
authors.initials: DH
authors.lastname: Kim
authors.forename: M K
authors.initials: MK
authors.lastname: Shin
authors.forename: Hun-Taeg
authors.initials: HT
authors.lastname: Chung
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D008518:Medicine, East Asian Traditional; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D029596:Paeonia / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D020298:U937 Cells
chemical_list: D000893:Anti-Inflammatory Agents; D018932:Chemokine CCL2; D016209:Interleukin-8; D010936:Plant Extracts; D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate