[Studies on method for the determination of kaerophyllin in Bupleurum smithii wolff Wolff var. parvifolium].
OBJECTIVE
To establish a quantitative method for the determination of kaerophyllin in Chinese medicine Bupleurum smithii ver. parvifolium.


METHODS
Kaerophyllin was determined by HPLC, the mobile phase consisted of methanol-water(70:30), the flow rate was 1 ml.min-1, the eluate was monitored with UV detector at 328 nm.


RESULTS
The average recovery was 97.1% with RSD = 2.15 (n = 5).


CONCLUSIONS
The result was precise and stable. This method was sensitive and its repeatability was excellent. This method may serve determination of kaerophyllin in B. smithii var. parvifolium.
Dou
H S
HS
School of Pharmaceutical Sciencn, West China University of Medical Sciences, Chengdu 610041, Sichuan, China.
Zeng
S X
SX
Wang
Y H
YH
Shang
M Y
MY
D029972:Bupleurum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
D004365:Drugs, Chinese Herbal