title: Temporomandibular joint synovial fluid sampling: estimation of dilution factor using calcium ion concentration.
abstract: Saline aspirates have been commonly used in the biochemical investigations of temporomandibular joint (TMJ) pathology. However, due to presence of adhesions in the diseased temporomandibular joint, full equilibration between the injected saline and the synovial fluid may not be achieved in all cases. We measured calcium ion concentration in the saline aspirates and the plasma to assess the degree of dilution of the synovial fluid by the injected media. Saline aspirates obtained prior to the arthroscopic examination of 17 patients with painful TMJs not responding to 3 months of conservative treatment were analysed for their calcium content by a highly sensitive spectrophotometric autoanalyser. In 10 patients with unilateral symptoms, the contralateral asymptomatic side was used as a control. Using a concentration volume equation the amount of the synovial fluid in the saline aspirates was calculated. The yield of the saline aspirates was variable ranging from 330 to 1000 microl. The mean calcium level was 0.787 mg/dl in the symptomatic group (C.I. 95% 0.337-1.237 mg/dl) and 0.512 mg/dl (C.I. 95% 0.235-0.797) in the asymptomatic group. Using a Student t-test there was no significant difference between the two groups. Furthermore, there was no demonstrable correlation between the volume of the aspirate and its synovial fluid content. This study confirms that the saline aspirate may not be a representative sample of the TMJ synovial fluid, and that expression of the results of the biochemical assays per volume of the aspirate may be misleading.
authors.affiliation: Department of Oral & Maxillofacial Surgery, Eastman Dental Hospital, 256 Gray's Inn Road, London WC1X 8LD, UK. B.Aghabeigi@eastman.ucl.ac.uk
authors.forename: B
authors.initials: B
authors.lastname: Aghabeigi
authors.forename: N
authors.initials: N
authors.lastname: Cintra
authors.forename: S
authors.initials: S
authors.lastname: Meghji
authors.forename: A
authors.initials: A
authors.lastname: Evans
authors.forename: S J
authors.initials: SJ
authors.lastname: Crean
mesh_terms: D000328:Adult; D000465:Algorithms; D001182:Arthroscopy; D015415:Biomarkers / Q000032:analysis; D002118:Calcium / Q000032:analysis* / Q000097:blood; D003124:Colorimetry; D016001:Confidence Intervals; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D019152:Paracentesis / Q000379:methods; D010635:Phenolphthaleins; D012965:Sodium Chloride; D013053:Spectrophotometry; D013223:Statistics as Topic; D013582:Synovial Fluid / Q000737:chemistry*; D013704:Temporomandibular Joint / Q000378:metabolism*; D013705:Temporomandibular Joint Disorders / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D007202:Indicators and Reagents; D010635:Phenolphthaleins; D012965:Sodium Chloride; C017845:2-cresolphthalexon; D002118:Calcium