title: [An adenosine triphosphate bioluminescence assay for detecting the number of living cells].
abstract: The method for detecting the number of living cells was studied. Using an adenosine triphosphate (ATP) bioluminescence assay, the present authors reported a perfect linear relationship between lg ATP concentrations and lg luminescence counts (r = 0.9963) as well as a relationship between lg number of cells and lg ATP luminescence counts (r = 0.9922). The detectable cells ranged from 10(2) to 10(6) cells/ml, the coefficients of variation 1-3%. This method is simple, accurate and sensitive and has a high reproducibility.
authors.affiliation: Department of Obstetrics and Gynecology, Second Affiliated Hospital, WCUMS, Chengdu 610041.
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.forename: Z
authors.initials: Z
authors.lastname: Peng
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Lou
authors.forename: B
authors.initials: B
authors.lastname: He
authors.forename: Q
authors.initials: Q
authors.lastname: Tang
authors.forename: D
authors.initials: D
authors.lastname: Qiu
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D002452:Cell Count / Q000379:methods; D005350:Fibroma / Q000473:pathology*; D008163:Luminescent Measurements; D051379:Mice; D014407:Tumor Cells, Cultured
chemical_list: D000255:Adenosine Triphosphate