title: Transient CENP-E-like kinetochore proteins in plants.
abstract: Derived from candidate sequences of a barley EST database two proteins with homology to the coiled coil region of the human kinetochore protein (KP) CENP-E were generated and classified as centromere protein E-like 1 and 2 (Cpell and Cpe12). Specific antibodies produced against recombinant Cpe11 and Cpe12 proteins labeled the centromere on mitotic chromosomes of barley and field bean and recognized specifically proteins from nuclear/chromosomal protein extracts on immunoblots. No function was predicted for homologues of Cpe11 within the databases for Arabidopsis and rice genomes. However, the centromeric location of Cpe11 and Cpe12 suggests they may have a function within the kinetochore. Plant homologues to barley Cpe12 are N-type kinesins, suggesting that Cpe12 is functionally homologous to human CENP-E.
authors.affiliation: Institute of Plant Genetics and Crop Plant Research, 06466 Gatersleben, Germany.
authors.forename: Rogier
authors.initials: R
authors.lastname: ten Hoopen
authors.forename: Thomas
authors.initials: T
authors.lastname: Schleker
authors.forename: Renate
authors.initials: R
authors.lastname: Manteuffel
authors.forename: Ingo
authors.initials: I
authors.lastname: Schubert
mesh_terms: D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002503:Centromere / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002875:Chromosomes / Q000378:metabolism; D017124:Conserved Sequence; D004926:Escherichia coli / Q000235:genetics; D001467:Hordeum / Q000235:genetics; D006801:Humans; D018386:Kinetochores / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism; D018519:Meristem / Q000166:cytology / Q000378:metabolism; D008938:Mitosis; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D031306:Vicia / Q000235:genetics
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D010940:Plant Proteins; D011994:Recombinant Proteins; C068688:centromere protein E