Cloned cytosine deaminase gene expression of Bifidobacterium longum and application to enzyme/pro-drug therapy of hypoxic solid tumors.
Bifidobacterium longum is a nonpathogenic anaerobic bacterium among normal bacterial flora. Recently, it was reported that B. longum accumulated in hypoxic solid tumors. The gene of interest was expressed in transfected B. longum by the shuttle vector pBLES100 in solid tumors. In this report, we constructed pBLES100-S-eCD, which included the cytosine deaminase gene. We confirmed by western blotting that transfected B. longum produced cytosine deaminase. In addition, transfected B. longum produced cytosine deaminase that converted 5-fluorocytosine into 5-fluorouracil. B. longum could be useful for enzyme/pro-drug therapy of hypoxic solid tumors.
Nakamura
Toshiyuki
T
Department of Surgery, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan.
Sasaki
Takayuki
T
Fujimori
Minoru
M
Yazawa
Kazuyuki
K
Kano
Yasunobu
Y
Amano
Jun
J
Taniguchi
Shun'ichiro
S
D000964:Antimetabolites, Antineoplastic / Q000493:pharmacokinetics* / Q000627:therapeutic use; D001644:Bifidobacterium / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D003001:Cloning, Molecular; D043525:Cytosine Deaminase; D005437:Flucytosine / Q000493:pharmacokinetics* / Q000627:therapeutic use; D005472:Fluorouracil / Q000493:pharmacokinetics* / Q000627:therapeutic use; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D000860:Hypoxia / Q000503:physiopathology; D009369:Neoplasms / Q000188:drug therapy; D009700:Nucleoside Deaminases / Q000235:genetics* / Q000378:metabolism*; D011355:Prodrugs / Q000493:pharmacokinetics* / Q000627:therapeutic use; D014162:Transfection; D014169:Transformation, Bacterial
D000964:Antimetabolites, Antineoplastic; D011355:Prodrugs; D005437:Flucytosine; D009700:Nucleoside Deaminases; D043525:Cytosine Deaminase; D005472:Fluorouracil