Isolation and characterization of cDNAs that encode homologs of a pathogenesis-related protein allergen from Cryptomeria japonica.
Many plant pathogenesis-related (PR) proteins are allergenic. We isolated three cDNAs, Cry j 3.1, Cry j 3.2, and Cry j 3.3, that encoded homologs of Jun a 3, a PR protein allergen in Juniperus ashei, from a cDNA library derived from the pollen of Cryptomeria japonica. The predicted amino acid sequences encoded by the three cDNAs were more than 85% identical to each other and about 57% identical to the sequence of Jun a 3. The Cry j 3 genes seemed to form a small multigene family in the genome of C. japonica. Expression of Cry j 3 was strong in roots and in female and male strobili; expression was weaker in cotyledons, leaves, stems, and pollen grains.
Futamura
Norihiro
N
Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute, Ibaraki 305-8687, Japan.
Mukai
Yuzuru
Y
Sakaguchi
Masahiro
M
Yasueda
Hiroshi
H
Inouye
Sakae
S
Midoro-Horiuti
Terumi
T
Goldblum
Randall M
RM
Shinohara
Kenji
K
D000485:Allergens / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D032485:Cryptomeria / Q000235:genetics / Q000276:immunology*; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification*; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D012313:RNA / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D000485:Allergens; D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D011994:Recombinant Proteins; D012313:RNA