Restriction analysis of the ITS region for characterization of the Debaryomyces species.
The internal transcribed spacer (ITS) region of rDNA was used for taxonomic inferences in ascomycetous yeasts. The Debaryomyces species had a 640-690 ITS size. The analyzed Candida species can be differentiated by its distinct ITS size. The enzymatic digestion of the ITS region show large homogeneity in Debaryomyces, with polymorphism for only two enzymes. The ITS size and the enzymatic restriction method were used in Brazilian isolates, detecting some Debaryomyces misidentifications in cultures previously identified by conventional methods.
Ramos
Jesus P.
JP
Departamento de Genética, Instituto de Biologia, CCS, bloco A, Prof. Paulo de Goes, Universidade Federal do Rio de Janeiro, Ilha do Fundao, Rio de Janeiro, CEP: 21944-970, Brasil.
Valente
Patricia
P
Hagler
Allen N.
AN
Leoncini
Orilio
O