Distinct expression of calnexin in major human salivary glands.
Calnexin (Cnx) has been characterized as a membrane-bound protein that transiently interacts in a unique chaperone system with newly synthesized glycoproteins in order to allow the establishment of their proper tertiary and, in most cases, quarternary structures. The aim of the study was to identify and to locate the expression of Cnx in the three major salivary glands of humans by different methods. Strong expression of Cnx protein and mRNA were generally found in serous salivary secretory units. With regard to mucous secretory units, expression of Cnx was only detectable at a low level in mucous acinar cells of sublingual glands, but not of submandibular glands. Expression of Cnx was always preserved in the surface epithelium of intralobar and interlobular duct segments. In addition, expression of Cnx was detected in sebaceous glands of parotid tissues, with a distribution pattern resembling that seen in sebaceous glands of the normal skin. In conclusion, production of saliva is associated with the expression of Cnx. Synthesis of molecules in mucous secretory units is not necessarily associated with a strong Cnx expression, whereas synthesis in serous secretory units apparently is. The tissue-specific Cnx expression is also paralleled by the observation that the secretions produced by the major salivary glands differ in their composition and amount.
Gassler
N
N
Institute of Pathology, University of Heidelberg, Germany,
Bohn
J
J
Schnölzer
M
M
Scheuerer
J
J
Obermüller
N
N
Otto
H F
HF
Autschbach
F
F
D015153:Blotting, Western; D037281:Calnexin / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D012469:Salivary Glands / Q000166:cytology / Q000378:metabolism*; D012627:Sebaceous Glands / Q000166:cytology / Q000378:metabolism
D037281:Calnexin