title: Peak compression effects in capillary electrochromatography of basic drug substances using a strong cation-exchanger.
abstract: Peak compression effects in capillary electrochromatography of basic drug substances using a strong cation-exchanger have been studied. Extremely narrow peaks with apparent efficiencies of several million plates per meter could be obtained when the composition of the sample zone differed from that of the mobile phase. The increased efficiencies were predominately observed when the analyte had an elution time similar to that of the electroosmotic flow marker. Peak compression was found to be reproducible and could be obtained for all investigated basic drug substances by altering the composition of the mobile phase in such a way that the analyte co-eluted with the sample zone. An explanation of the observed phenomena is proposed. A sample zone differing in composition from the mobile phase will disturb the equilibrium between the stationary and mobile phase. The elution rate of an analyte will consequently be different when residing inside the sample zone. If the analyte migrates through the sample zone at a higher speed than the rest of the mobile phase and is strongly retained after passing through a boundary in the sample zone, a continuous stacking can be obtained trapping the analyte as a very narrow band.
authors.affiliation: AstraZeneca, Process R&D, Department of Analytical Chemistry, S-151 85 Södertälje, Sweden. anna-maria.enlund@astrazeneca.com
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Enlund
authors.forename: Margareta E
authors.initials: ME
authors.lastname: Andersson
authors.forename: Gunnar
authors.initials: G
authors.lastname: Hagman
mesh_terms: D002411:Cation Exchange Resins; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results
chemical_list: D002411:Cation Exchange Resins; D004364:Pharmaceutical Preparations