title: Identification of a MAP65 isoform involved in directional expansion of plant cells.
abstract: MAP65 comprises a multigene family specific to plants. To see which isoform is utilised for the unique mechanism of cell expansion, uncomplicated by division structures, carrot cells were deprived of auxin whereupon they stopped dividing and elongated instead. During elongation, a MAP65 protein triplet reduced to a single band. Mass spectrometric analysis demonstrated that this corresponded to a single carrot cDNA; it also corresponded to the major protein previously shown to form filamentous cross-bridges between microtubules in vitro. This MAP65 isoform is concluded to have a major role in establishing the parallel microtubule arrays characteristic of cells undergoing directional expansion.
authors.affiliation: Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK.
authors.forename: Jordi
authors.initials: J
authors.lastname: Chan
authors.forename: Guojie
authors.initials: G
authors.lastname: Mao
authors.forename: Andrei
authors.initials: A
authors.lastname: Smertenko
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Hussey
authors.forename: Mike
authors.initials: M
authors.lastname: Naldrett
authors.forename: Andrew
authors.initials: A
authors.lastname: Bottrill
authors.forename: Clive W
authors.initials: CW
authors.lastname: Lloyd
mesh_terms: D000595:Amino Acid Sequence; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000302:isolation & purification; D018552:Daucus carota / Q000166:cytology / Q000235:genetics; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D059828:Plant Cells; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D010944:Plants / Q000235:genetics; D020033:Protein Isoforms; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D018076:DNA, Complementary; D008869:Microtubule-Associated Proteins; D010940:Plant Proteins; D020033:Protein Isoforms