title: [Influence of escharectomy and skin grafting during early burn stage on acute-phase response in severely burned rats and its significance].
abstract: OBJECTIVE
  To investigate the influence of escharectomy and skin-grafting during early burn stage on the production of acute-phase reactants by the liver in severely burned rats.
  
  
  METHODS
  Ninety-six Wistar rats were randomly divided into three groups: normal control group (C), escharectomy on postburn day (PBD) 1 group (0) and escharectomy on PBD4 group (F). 30% III degrees (total body surface area full-thickness) burn injury was caused by immersing the back in boiling water for 10 seconds. Escharectomy and skin grafting with cryopreserved allogeneic skin were performed one day or 4 days after the burn injury. ON days 1, 2, 4, 5 and 7 after burn injury blood was withdrawn from the abdominal aorta of 8 rats for each experimental group. The serum contents al-antitrypsin (AT), al-acid glycoprotein (AGP), C-reactive protein (CRP), albumin (Alb), and transferrin (Trf) were measured by turbidimetry.
  
  
  RESULTS
  Significant increase in concentrations of positive acute-phase reactant, CRP, AGP, and AT, was observed in group O and group F as compared with normal control, (P < 0.05), while the concentrations of negative acute-phase reactants, Alb and Trf, decreased markedly (P < 0.05). The serum concentrations of AT and AGP in group O began to decrease significantly on PBD 5 and PBD7 respectively in comparison with that of the group F (P < 0.05), however, they still maintained a rather higher levels on PBD7. The concentration of CRP in group O began to decrease on PBD4, and the values on PBD4, 5, and 7 were significantly lower than those of the group F (P < 0.05). The concentration of CRP in PBD1 group reached the normal level on PBD7, however, the concentration of CRP in group F kept increasing till PBD5, and remained significantly higher than that in group C (P < 0.05). The concentration of Alb in group O was significantly higher on PBD7 than that in group F (P < 0.05). The concentration of Trf in group O on PBD5 and PBD7 was significantly higher than those in group F (P < 0.05).
  
  
  CONCLUSIONS
  Escharectomy and skin grafting during early burn stage attenuates acute-phase response after burn injury and are beneficial to the organism.
authors.affiliation: Department of Burn and Plastic Surgery, 304th Hospital of Chinese People's Liberation Army, Beijing 100037, China.
authors.forename: Jiake
authors.initials: J
authors.lastname: Chai
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Wu
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Sheng
mesh_terms: D000210:Acute-Phase Reaction / Q000097:blood / Q000209:etiology / Q000601:surgery*; D000818:Animals; D002056:Burns / Q000097:blood / Q000150:complications / Q000601:surgery*; D002097:C-Reactive Protein / Q000378:metabolism; D008297:Male; D009961:Orosomucoid / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000378:metabolism; D016038:Skin Transplantation; D013997:Time Factors; D014168:Transferrin / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000378:metabolism
chemical_list: D009961:Orosomucoid; D012709:Serum Albumin; D014168:Transferrin; D000515:alpha 1-Antitrypsin; D002097:C-Reactive Protein