Calcium-deficient apatite: influence of granule size and consolidation mode on release and in vitro activity of vancomycin.
The use of dynamic compaction and isostatic compression to consolidate calcium phosphate powder loaded with a therapeutic agent avoids a sintering step that could destroy the drug. The present study applied these consolidation methods to vancomycin-loaded calcium-deficient apatite powder, using three granulometric fractions (40-80, 80-200 and 200-500 micrometer). In vitro release profiles were determined via an original system derived from low-pressure liquid chromatography. The biological activity of vancomycin was measured by an in vitro standardized bacteriologic assay, which showed that the drug is completely active after association with calcium phosphate. Regardless of the consolidation method and granulometric fraction used, release profiles were not significantly different and therefore adaptable to injectable suspensions.
Obadia
L
L
Centre de Recherche INSERM 99-03 sur les Matériaux d'Intérêt Biologique, EM INSERM 99-03, BP 84125, Cedex 44042, Nantes, France. laetitia.obadia@sante.univ-nantes.fr
Amador
G
G
Daculsi
G
G
Bouler
J-M
JM
D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D001031:Apatites / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D002118:Calcium / Q000737:chemistry*; D004339:Drug Compounding; D016503:Drug Delivery Systems; D066298:In Vitro Techniques; D014640:Vancomycin / Q000008:administration & dosage* / Q000737:chemistry
D000900:Anti-Bacterial Agents; D001031:Apatites; D001672:Biocompatible Materials; D014640:Vancomycin; D002118:Calcium