One step purification of alpha(1)-acid glycoprotein from human plasma. Fractionation of its polymorphic allele products.
Alpha(1)-acid glycoprotein is a plasma protein that exhibits both microheterogeneity and polymorphism. Its purification from human plasma is usually performed using a sequence of different fractionation steps. Here we report a one-step isolation technique of this protein based upon pseudo-ligand affinity chromatography on immobilized Cibacron Blue F3GA at acidic pH. In addition, the use of two narrow pH elution buffers allows us to separate the two genetic products of this protein, which differ from each other by 21 amino acid substitutions. This technique will facilitate the study of the structural, biological and pharmacokinetic properties of each individual allele product.
Azzimonti
Federica
F
Department of Microbiology and Immunology, Medical University of South Carolina, 29425, Charleston, SC, USA.
Atchley
Daniel H
DH
Morrison
Catherine Anne
CA
Dodd
Seetal
S
Boulton
David W
DW
DeVane
C Lindsay
CL
Arnaud
Philippe
P
D000483:Alleles; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D009961:Orosomucoid / Q000235:genetics / Q000302:isolation & purification*; D011110:Polymorphism, Genetic
D009961:Orosomucoid