Components with potential immunosuppressive activity in lipopolysaccharide of laboratory Pseudomonas aeruginosa strains.
The ability of culture filtrate (CF) of two laboratory Pseudomonas aeruginosa strains to inhibit delayed type hypersensitivity (DTH) to non-bacterial antigen in CBA mice has been studied. It was shown that intraperitoneal injection of native CF of the strains did not affect the level of DTH. However, redox treated CF expressed the immunosuppressive activity. Gel filtration of redox activated CF through Sephadex G-200 showed that CF contains three immunosuppressive components differing by their molecular weight and specificity. All components contained lipid group and O-polysaccharide chains that indicated their lipopolysaccharide (LPS) nature. These experiments show that laboratory P. aeruginosa strains have three LPS components but not all of them display the immunosuppressive activity.
Vikström
Elena V
EV
Division of Medical Microbiology, Department of Molecular and Clinical Medicine, Linköping University, SE-581 85, Linköping, Sweden. elevi@imk.liu.se
D000818:Animals; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007109:Immunity / Q000187:drug effects*; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011550:Pseudomonas aeruginosa / Q000737:chemistry*
D007166:Immunosuppressive Agents; D008070:Lipopolysaccharides