Sample matrix influence on the choice of background electrolyte for the analysis of bases with capillary zone electrophoresis.
Low levels of impurities need to be determined in drugs. Consequently, if UV detection is used, a large sample amount must be loaded and as narrow peaks as possible obtained. The sample matrix and the stability of the samples as well as the peak resolution should be considered when the electrolyte is chosen. In this study the influence of the sample matrix composition with varying background electrolytes on the peak appearance of model mixtures loaded in large amounts was investigated. A robust electrolyte for analysis of bases in a sample with varying pH was found to consist of a buffering co-ion and a buffering counter-ion (the pH was approximately 4.2 in the electrolyte). If a minor component has higher mobility than the macrocomponent and the co-ion, better peak shape can be obtained if, for instance, enough sodium chloride is added to the sample, i.e., sample self-stacking is exploited. The effect of addition of organic modifiers, isopropanol or acetonitrile, was examined and good linearity and precision have been shown for impurities in the concentration range tested, approximately 0.03 to 5 mol% of the main component, in model mixtures.
Andersson
E K Margareta
EK
Product Analysis, Pharmaceutical and Analytical R&D, AstraZeneca, SE-151 85 Södertälje, Sweden. margareta.andersson@astrazeneca.com
Hägglund
Ingela
I
D002021:Buffers; D004573:Electrolytes / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D013056:Spectrophotometry, Ultraviolet
D002021:Buffers; D004573:Electrolytes