title: Preliminary X-ray crystallographic analysis of tRNA pseudouridine 55 synthase from the thermophilic eubacterium Thermotoga maritima.
abstract: Thermotoga maritima TruB, an enzyme responsible for the formation of pseudouridine in tRNA, has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li(2)SO(4), 20% glycerol, 13% PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 47.39, b = 83.88, c = 98.72 A, and diffract to 2.0 A resolution using synchrotron radiation. A solution was obtained by molecular replacement using part of the recently published crystal structure of Escherichia coli TruB bound to a synthetic RNA.
authors.affiliation: Institut de Recherches Microbiologiques JM Wiame, Belgium. jwouters@dbm.ulb.ac.be
authors.forename: Johan
authors.initials: J
authors.lastname: Wouters
authors.forename: Catherine
authors.initials: C
authors.lastname: Tricot
authors.forename: Virginie
authors.initials: V
authors.lastname: Durbecq
authors.forename: Martine
authors.initials: M
authors.lastname: Roovers
authors.forename: Victor
authors.initials: V
authors.lastname: Stalon
authors.forename: Louis
authors.initials: L
authors.lastname: Droogmans
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D019753:Intramolecular Lyases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019751:Intramolecular Transferases; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D020124:Thermotoga maritima / Q000201:enzymology* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D019751:Intramolecular Transferases; C096995:pseudouridine synthases; D019753:Intramolecular Lyases