PCR analysis of Yq microdeletions in infertile males, a study from South India.
OBJECTIVE
To estimate the frequency of microdeletions in the long arm of Y-chromosome of 20 infertile males from South India.


METHODS
Polymerase chain reaction (PCR) amplification using Y-specific STS of azoospermia factor (AZF) regions i.e., SY 84 for AZFa, SY 127 for AZFb and SY 254 for AZFc.


RESULTS
Of the 20 infertile subjects 3 (15 %), one azoospermic and two oligozoospermic, showed microdeletions in the AZF region of Y-chromosome.


CONCLUSIONS
The frequency of deletions involving AZF region of the Y-chromosome is 15 % in azoospermic and severely oligozoospermic infertile men. PCR amplification of AZF locus is useful for the diagnosis of microdeletions in the Y-chromosome.
Babu
S Ramesh
SR
Institute of Genetics & Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad-500 016, India.
Swarna
M
M
Padmavathi
P
P
Reddy
P P
PP
D001483:Base Sequence; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D041322:Chromosomes, Human, Y; D017931:DNA Primers; D005260:Female; D005787:Gene Frequency; D056426:Genetic Loci; D006801:Humans; D007194:India; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D009845:Oligospermia / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012016:Reference Values; D029589:Seminal Plasma Proteins / Q000235:genetics
D017931:DNA Primers; D029589:Seminal Plasma Proteins