title: Microarray reveals differences in both tumors and vascular specific gene expression in de novo CD5+ and CD5- diffuse large B-cell lymphomas.
abstract: Malignant lymphoma is a heterogeneous disease with different clinical features. Among diffuse large B-cell lymphomas (DLBCLs), a unique subtype has been identified recently based on cell surface marker CD5 and clinicopathological features. These de novo CD5(+) DLBCLs account for approximately 10% of all of the DLBCLs and have poorer prognosis. To additionally understand this subtype of DLBCLs at the molecular level and to find genes that are differentially expressed in de novo CD5(+) DLBCLs, CD5(-) DLBCLs, and mantle cell lymphomas, which also have poor prognosis, we performed gene expression profiling using cDNA microarray technology. Data from a total of 9 samples of CD5(-) DLBCLs, 11 samples of de novo CD5(+) DLBCLs, and 10 samples of mantle cell lymphomas were acquired. A series of genes were identified that distinguish these three types of lymphomas. Among DLBCL cases, integrin beta1 and/or CD36 adhesion molecules were overexpressed in most cases of CD5(+) DLBCL. An immunohistochemical confirmation study revealed that integrin beta1 was expressed on lymphoma cells, which may account for the high extranodal involvement and poor prognosis of CD5(+) DLBCLs. In contrast, CD36 was overexpressed on vascular endothelia in CD5(+) DLBCLs, although there was no difference in vascularity detected by von Wilbrand factor antibody between CD5(+) and CD5(-) DLBCLs. Those results suggest that CD5(+) and CD5(-) DLBCLs have different gene expression signatures in both tumor cells and their vascular systems.
authors.affiliation: The Second Department of Internal Medicine, Mie University School of Medicine, Tsu, 514-8507, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Motoko
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Seungchan
authors.initials: S
authors.lastname: Kim
authors.forename: Jun
authors.initials: J
authors.lastname: Morikawa
authors.forename: Shoko
authors.initials: S
authors.lastname: Ogawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ueno
authors.forename: Edward
authors.initials: E
authors.lastname: Suh
authors.forename: Edward
authors.initials: E
authors.lastname: Dougherty
authors.forename: Ilya
authors.initials: I
authors.lastname: Shmulevich
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shiku
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001808:Blood Vessels / Q000473:pathology*; D018956:CD5 Antigens / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016130:Immunophenotyping; D016393:Lymphoma, B-Cell / Q000145:classification / Q000235:genetics* / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000145:classification / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D014841:von Willebrand Factor / Q000235:genetics
chemical_list: D018956:CD5 Antigens; D014841:von Willebrand Factor