Endocrine cells with gastrin/cholecystokinin-like immunoreactivity in the pancreas of the spiny dogfish, Squalus acanthias.
Endocrine cells containing gastrin/cholecystokinin (CCK)-like immunoreactivity were localized to the islet tissue in the pancreas of the spiny dogfish. Most of these cells were located in the 'intestinal' lobe of the pancreas; only occasional cells were observed in the 'splenic' lobe. The gastrin/CCK-like immunoreactive cells were often co-localized with the 'classical' pancreas hormones (insulin, glucagon and somatostatin). Radioimmunoassay of water extracts with a C-terminally directed antiserum revealed high levels of immunoreactive material in the intestinal part (48.6 +/- 19.9 pmol/g) and lower levels (4.5 +/- 0.6 pmol/g) in the splenic part. Acetic acid extracts of the intestinal lobe contained low levels (6.8 +/- 3.3 pmol/g) of gastrin/CCK-like immunoreactivity, whereas corresponding extracts of the splenic part showed no immunoreactivity. When the extracts were subjected to DEAE ion-exchange chromatography the gastrin/CCK-like peptides eluted as a major peak. After Sephadex gel filtration, pooled immunoreactive material from the main DEAE chromatographic peak eluted at a position close to that of CCK4. Further characterization by ion-exchange and reversed-phase HPLC showed that, in general, the immunoreactive material behaved like the shorter forms of the gastrin/CCK family (CCK4/G5 and CCK8/Cae 3-10).
Jönsson
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AC
Comparative Neuroscience Unit, Department of Zoophysiology, University of Göteborg, Medicinaregatan 18, S-413 90 Göteborg, Sweden.
D000818:Animals; D002766:Cholecystokinin / Q000032:analysis*; D004284:Dogfish; D004702:Endocrine Glands; D019858:Enteroendocrine Cells / Q000737:chemistry / Q000166:cytology*; D005755:Gastrins / Q000032:analysis*; D007150:Immunohistochemistry / Q000379:methods; D010179:Pancreas / Q000737:chemistry / Q000166:cytology*; D011863:Radioimmunoassay
D005755:Gastrins; D002766:Cholecystokinin