title: Role of heme oxygenase-1 in morphine-modulated apoptosis and migration of macrophages.
abstract: We examined the role of heme oxygenase (HO)-1 in morphine-induced decrease in macrophage migration. Morphine promoted expression of HO-1 in murine macrophages. Morphine-receiving mice (MRCs) showed decreased (P<.001) macrophage migration and increased (P<.001) occurrence of macrophage apoptosis. In in vitro studies, peritoneal macrophages harvested from MRCs also showed decreased (P<.001) migration, compared with those from control mice. Bone marrow cells isolated from MRCs showed not only decreased (P<.001) migration but also increased apoptosis. Pretreatment of MRCs with hemin not only decreased migration of macrophages further but also enhanced the apoptosis of peritoneal macrophages. On the other hand, pretreatment of MRCs with zinc protoporphyrin attenuated the effect of morphine on both macrophage migration and the occurrence of apoptosis. In in vitro studies, pretreatment of macrophages with hemin exacerbated morphine-induced apoptosis, whereas pretreatment with zinc protoporphyrin attenuated morphine-induced macrophage apoptosis.
authors.affiliation: Department of Medicine, Long Island Jewish Medical Center, New Hyde Park, New York 11040, USA.
authors.forename: Kalpesh
authors.initials: K
authors.lastname: Patel
authors.forename: Madhu
authors.initials: M
authors.lastname: Bhaskaran
authors.forename: Dhimant
authors.initials: D
authors.lastname: Dani
authors.forename: Krishna
authors.initials: K
authors.lastname: Reddy
authors.forename: Pravin C
authors.initials: PC
authors.lastname: Singhal
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D018932:Chemokine CCL2 / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006419:Heme Oxygenase (Decyclizing) / Q000502:physiology*; D051547:Heme Oxygenase-1; D006427:Hemin / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D009020:Morphine / Q000494:pharmacology*
chemical_list: D018932:Chemokine CCL2; D008565:Membrane Proteins; D006427:Hemin; D009020:Morphine; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse