Evaluation of MicroScan Autoscan for identification of Pseudomonas aeruginosa isolates from cystic fibrosis patients.
Accurate identification of gram-negative bacilli from cystic fibrosis (CF) patients is essential. Only 57% (108 of 189) of nonmucoid strains and 40% (24 of 60) of mucoid strains were definitively identified as Pseudomonas aeruginosa with MicroScan Autoscan. Most common misidentifications were Pseudomonas fluorescens-Pseudomonas putida (i.e., the strain was either P. fluorescens or P. putida, but the system did not make the distinction and yielded the result P. fluorescens/putida) and Alcaligenes spp. Extending the incubation to 48 h improved identification, but 15% of isolates remained misidentified. The MicroScan Autoscan system cannot be recommended for the identification of P. aeruginosa isolates from CF patients.
Saiman
Lisa
L
Department of Pediatrics, Division of Infectious Diseases, Columbia University, New York, New York 10032, USA. ls5@columbia.edu
Burns
Jane L
JL
Larone
Davise
D
Chen
Yunhua
Y
Garber
Elizabeth
E
Whittier
Susan
S
D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic
D011933:Reagent Kits, Diagnostic