Membrane biochips.
Membrane-bound proteins represent the single most important class of drug targets. This article discusses the issues surrounding fabrication of membrane-protein microarrays by conventional robotic pin printing techniques. Ligand binding selectivity and specificity to G protein-coupled receptor (GPCR) microarrays are presented. The potential applications of these arrays for drug screening are discussed.
Fang
Ye
Y
Science and Technology Division, Corning Incorporated, Corning, NY, USA.
Frutos
Anthony G
AG
Webb
Brian
B
Hong
Yulong
Y
Ferrie
Ann
A
Lai
Fang
F
Lahiri
Joydeep
J
D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D019204:GTP-Binding Proteins / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D008565:Membrane Proteins / Q000737:chemistry; D008567:Membranes, Artificial; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000032:analysis / Q000737:chemistry; D018342:Receptors, Adrenergic, beta-1 / Q000032:analysis / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000737:chemistry; D017447:Receptors, Dopamine D1 / Q000032:analysis / Q000737:chemistry; D018028:Receptors, Neurotensin / Q000032:analysis / Q000737:chemistry
D008024:Ligands; D008565:Membrane Proteins; D008567:Membranes, Artificial; D011506:Proteins; D018342:Receptors, Adrenergic, beta-1; D011956:Receptors, Cell Surface; D017447:Receptors, Dopamine D1; D018028:Receptors, Neurotensin; C114036:neurotensin type 1 receptor; D019204:GTP-Binding Proteins