title: Antibody recognition of chiral surfaces. Enantiomorphous crystals of leucine-leucine-tyrosine.
abstract: Monoclonal antibodies were selected after immunization with crystals of the tripeptide l-leucine-l-leucine-l-tyrosine. They interact with the tripeptide crystals, but do not interact with the tripeptide molecule, with other crystalline surfaces, or with adsorbed protein. The interactions of two antibodies with crystals of l-Leu-l-Leu-l-Tyr and of its enantiomer d-Leu-d-Leu-d-Tyr were characterized in depth. Antibody 48E is stereoselective and enantioselective: it recognizes only the [011] faces of the l-Leu-l-Leu-l-Tyr crystals, and not the enantiomorphous [011] faces of d-Leu-d-Leu-d-Tyr crystals, or any other faces of either crystal. In contrast, antibody 602E is poorly stereoselective and is not enantioselective: it recognizes the crystals of both enantiomers, interacting with a number of different faces of each. The different recognition patterns are explained on the basis of the nature of the interactions and the structure of the interacting surfaces. Understanding this antibody specificity advances our general understanding of surface recognition and transfer of chiral information across biological interfaces.
authors.affiliation: Department of Structural Biology, Weizmann Institute of Science, 76100 Rehovot, Israel.
authors.forename: Merav
authors.initials: M
authors.lastname: Geva
authors.forename: Felix
authors.initials: F
authors.lastname: Frolow
authors.forename: Miriam
authors.initials: M
authors.lastname: Eisenstein
authors.forename: Lia
authors.initials: L
authors.lastname: Addadi
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology*; D000917:Antibody Formation; D000918:Antibody Specificity; D003460:Crystallization; D018360:Crystallography, X-Ray; D005260:Female; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry* / Q000276:immunology*; D011487:Protein Conformation; D013237:Stereoisomerism; D013499:Surface Properties
chemical_list: D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D009842:Oligopeptides; C062538:leucyl-leucyl-tyrosine