Monkey embryonic stem cell lines expressing green fluorescent protein.
The major limitation of nonhuman primate (NHP) embryonic stem (ES) cell research is inefficient genetic modification and limited knowledge of differentiation mechanisms. A genetically modified NHP-ES cell with biomarkers, such as green fluorescent protein (GFP), that allow noninvasive monitoring of transgenic cells, is a useful tool to study cell differentiation control during preimplantation and fetal development, which also plays a crucial role in the development of cell transplantation medicine. Here we report the establishment of transgenic NHP-ES cell lines that express GFP without jeopardizing their pluripotency, which was confirmed by in vitro and in vivo differentiation. These GFP-expressing ES cells reproducibly differentiated into embryoid bodies, neural cells, and cardiac myocytes. They formed teratoma composed of tissues derived from the three embryonic germ layers when transplanted into severe combined immunodeficient disease (SCID) mice. GFP expression was maintained in these differentiated cells, suggesting that these cells were useful for cell transplantation experiments. Furthermore, we showed that these ES cells have the ability to form chimeric blastocysts by introducing into the early preimplantation stage NHP embryo.
Takada
Tatsuyuki
T
Department of Experimental Radiology, Shiga University of Medical Science, Ohtsu, Shiga, 520-2192, Japan.
Suzuki
Yutaka
Y
Kondo
Yasushi
Y
Kadota
Nae
N
Kobayashi
Kinji
K
Nito
Shinji
S
Kimura
Hiroshi
H
Torii
Ryuzo
R
D000818:Animals; D015415:Biomarkers; D018929:Cell Culture Techniques / Q000379:methods* / Q000639:trends; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D005855:Germ Layers / Q000166:cytology / Q000378:metabolism; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000096:biosynthesis*; D008252:Macaca fascicularis; D051379:Mice; D016513:Mice, SCID; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation; D033581:Stem Cell Transplantation / Q000379:methods* / Q000639:trends; D013724:Teratoma / Q000378:metabolism / Q000473:pathology; D018183:Transplantation Chimera / Q000196:embryology / Q000378:metabolism
D015415:Biomarkers; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins