Simultaneous determination of creatinine and pseudouridine concentrations in bovine plasma by reversed-phase liquid chromatography with photodiode array detection.
A simple, rapid method for the simultaneous determination of creatinine and pseudouridine in bovine plasma is described. Plasma was de-proteinised, concentrated, and chromatographed for 15 min on a C(18) column. Analytes were detected at an optimum wavelength (262 nm) and the internal standard (cimetidine) was detected at 220 nm. The pH of analysis was between 6.5 and 7 where both analytes exist in single chemical forms giving maximum accuracy. Recoveries of both analytes were above 96%. Lowest detectable amounts of creatinine and pseudouridine were 0.28 nmol and 9.0 pmol, and the typical levels detected (+/-SD) were 60 (+/-2.8) and 2.3 (+/-0.10) micromol/L, respectively.
Hewavitharana
A K
AK
Cooperative Research Centre for Cattle and Beef Quality, CSIRO, Food Science Australia, 4170, Cannon Hill, Queensland, Australia. a.hewavitharana@foodscience.afisc.csiro.au
Bruce
H L
HL
D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D003404:Creatinine / Q000097:blood*; D011560:Pseudouridine / Q000097:blood*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D011560:Pseudouridine; D003404:Creatinine