title: Molecular diversity, new species and antifungal susceptibilities in the Exophiala spinifera clade.
abstract: Black yeasts morphologically identified as Exophiala cf. spinifera were re-analyzed on the basis of morphology, nutritional physiology and ITS rDNA sequencing data. Ribosomal small subunit (SSU) DNA sequence data confirmed that strains belonged to a clade containing the ex-type strain of E. spinifera. Variation in the Internal Transcribed Spacer (ITS) region of the rDNA was studied within this group. Five infraspecific groups were delineated. Phylogenetic analysis of the SSU rDNA data revealed that several of the strains analyzed were located at considerable evolutionary distance from the E. spinifera clade. ITS rDNA sequencing showed that the deviating strains could not be identified with any existing species. Two new species, E. attenuata and E. nishimurae, were therefore proposed. Antimycotic susceptibility data of the new species were compared with those obtained with members of the E spinifera clade.
authors.affiliation: Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands.
authors.forename: R G
authors.initials: RG
authors.lastname: Vitale
authors.forename: G S
authors.initials: GS
authors.lastname: de Hoog
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D005093:Exophiala / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal