[Apoptosis of K562 cells induced by antisense-oligonucleotides of CRKL gene].
OBJECTIVE
It was recently reported that the proteins coded by CRKL (CT10 regulator of kinase like, CRK like) gene play an important role in the pathogenesis of chronic myeloid leukemia (CML). This study was designed to investigate the effect of antisense oligonucleotides of CRKL gene (CRKL-ASDON) on CML K562 cell lines.


METHODS
K562 cells were transfected with CRKL-ASDON, using liposome as the vector. The changes of cell morphology, cell cycle, and gene expression were observed through living cell count, transmission electron microscopy, flow cytometry (FCM), DNA Ladder assay.


RESULTS
Under the action of CRKL-ASDON, growth of K562 cell was markedly inhibited. An apoptotic peak appeared before diploid peak in FCM; various apoptotic stages of K562 cells were observed under electron microscope; over ladder-shaped band of cell apoptosis was seen in abstracted DNA of the gene group by gel electrophoresis. Meanwhile, the above changes did not appear in the control.


CONCLUSIONS
CRKL gene is an important factor in the pathogenesis of Ph-positive CML.
Wang
Sha
S
Department of Hematology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, P. R. China.
Sun
Bing-zhong
BZ
Yu
Wen-qiang
WQ
Zhang
Tao
T
Feng
Qi
Q
Shang
Zhen-chuan
ZC
D048868:Adaptor Proteins, Signal Transducing; D017209:Apoptosis; D002452:Cell Count; D002455:Cell Division; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells / Q000648:ultrastructure; D008854:Microscopy, Electron; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000502:physiology; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D014162:Transfection
D048868:Adaptor Proteins, Signal Transducing; C082869:CRKL protein; D004273:DNA, Neoplasm; D009687:Nuclear Proteins; D016376:Oligonucleotides, Antisense