title: Evaluation of the cytotoxicity effect of dimethyl sulfoxide (DMSO) on Caco2/TC7 colon tumor cell cultures.
abstract: Dimethyl sulfoxide (DMSO) is usually used to solubilize poorly soluble drugs in permeation assays such as that using Caco2 enterocyte-like cells. The objective of this study was to evaluate the toxicity of DMSO on Caco2/TC7 cells and determinate the maximal concentration usable in permeation experiments. Caco2/TC7 cells were cultured for 21 d on 96-well plates for evaluation of toxicity. The determination of lactate dehydrogenase (LDH) release in cell supernatant and the measurement of Neutral Red (NR) uptake are used for cytotoxicity assays. DMSO solutions (0-100%) in Hank's balanced salt solution containing HEPES (25 mM), pH 7.4, were incubated with Caco-2/TC7 cells on 96 well plates. Caco2/TC7 cells were cultured on Transwell-Clear inserts to evaluate the influence of DMSO on the apparent permeability of the paracellular marker mannitol. DMSO 10% did not induce any significant increase in LDH release whereas a significant increase in LDH activity (ANOVA, p<0.05) occurred at a DMSO concentration of 20 to 50%. NR incorporation in viable cells was statistically reduced by 27 to 36% at DMSO concentration of 20% up to 100% (ANOVA, p>0.05). No statistical difference (p<0.05) in apparent mannitol permeability was observed between the control and 10% DMSO groups. In conclusion, at concentrations of up to 10%, DMSO did not produce any significant alteration in apical membrane permeability or on cell-to-cell tight junctional complexes.
authors.affiliation: Laboratoire GlaxoSmithkline, New Chemical Entity Unit, Evreux Cedex, France.
authors.forename: Georges
authors.initials: G
authors.lastname: Da Violante
authors.forename: Naima
authors.initials: N
authors.lastname: Zerrouk
authors.forename: Isabelle
authors.initials: I
authors.lastname: Richard
authors.forename: Gérard
authors.initials: G
authors.lastname: Provot
authors.forename: Jean Claude
authors.initials: JC
authors.lastname: Chaumeil
authors.forename: Philippe
authors.initials: P
authors.lastname: Arnaud
mesh_terms: D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D018938:Caco-2 Cells / Q000187:drug effects* / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D003110:Colonic Neoplasms / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000493:pharmacokinetics / Q000633:toxicity*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006801:Humans; D015203:Reproducibility of Results
chemical_list: D004121:Dimethyl Sulfoxide