[Feasibility of calcium polyphosphate fiber as scaffold materials for tendon tissue engineering in vitro].
OBJECTIVE
To study the feasibility of calcium polyphosphate fiber (CPPF) as the scaffold material of tendon tissue engineering.


METHODS
CPPF (15 microns in diameter) were woven to form pigtail of 3 mm x 2 mm transverse area; and the tensile strength, porous ratio and permeability ratio were evaluated in vitro. Tendon cells (5 x 10(4)/ml) derived from phalangeal flexor tendon of SD rats were co-culture with CPPF scaffold or CPPF scaffold resurfaced with collagen type-I within 1 week. The co-cultured specimens were examined under optical and electric scanning microscope.


RESULTS
The tensile strength of CPPF scaffolds was (122.80 +/- 17.34) N; permeability ratio was 61.56% +/- 14.57%; and porous ratio was 50.29% +/- 8.16%. CPPF had no obvious adhesive interaction with tendon cells, while CPPF of surface modified with collagen type-I showed good adhesive interaction with tendon cells.


CONCLUSIONS
The above results show that CPPF has some good physical characteristics as scaffold of tendon tissue engineering, but its surface should be modified with organic substance or even bioactive factors.
Sun
Zheng-yi
ZY
Orthopaedic Institute of Lanzhou Medical College, Lanzhou Gansu, P. R. China 730030.
Zhao
Lin
L
D000818:Animals; D001672:Biocompatible Materials; D002130:Calcium Phosphates; D002448:Cell Adhesion; D002478:Cells, Cultured; D005240:Feasibility Studies; D011108:Polymers; D051381:Rats; D017207:Rats, Sprague-Dawley; D013710:Tendons / Q000166:cytology*; D023822:Tissue Engineering
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011108:Polymers