Activity of phospholipase A2 with different localization in liposomes.
Damages to liposomal membranes resulted from intensification of lipid peroxidation with iron ions in the presence of ascorbate or treatment with the membrane disintegrator tetrachloromethane led to activation of phospholipase A2, which depended on the enzyme localization in liposomes. Activity of phospholipase A2 located on the inner liposome surface increased less significantly compared to the enzyme bound to the outer surface.
Karmalita
E G
EG
Department of Biochemistry and Molecular Biology, Laboratory of Biochemistry, Central Research Laboratory, Siberian State Medical University, Tomsk. ekarmalita@mail.ru
Serebrov
V Yu
VY
Novitskii
S V
SV
Novitskaya
T V
TV
Vavilkin
D A
DA
D001205:Ascorbic Acid / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D015227:Lipid Peroxidation; D008081:Liposomes / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D013696:Temperature; D024505:Tocopherols / Q000378:metabolism
D005227:Fatty Acids; D008081:Liposomes; D002251:Carbon Tetrachloride; D007501:Iron; D010741:Phospholipases A; D054467:Phospholipases A2; D001205:Ascorbic Acid; D024505:Tocopherols