[Detection and partial nucleotide sequence analysis of hepatitis G virus in sera of patients with hepatitis in Guangzhou].
OBJECTIVE
To investigate hepatitis G virus (HGV) infection in hepatitis patients and the gene type of HGV in Guangzhou.


METHODS
Reverse transcriptase-nested polymerase chain reaction(RT-nested PCR) method using primers locating in noncoding region of HGV genome was used for detecting HGV RNA in sera of hepatitis patients and the amplified products were directly sequenced.


RESULTS
25 cases were HGV RNA positive in 251 sera of acute and chronic hepatitis(9.96%), of which 4 cases were positive in 56 sera with hepatitis non A-E(7.14%), 8 cases were positive in 77 sera with hepatitis B (10.39%), 13 cases were positive in 118 sera with hepatitis C(11.17%). The identity of nucleotide sequence was 98.0% between 2 Guangzhou HGV isolates, both were 91.1% with American isolate, 81.7% and 84.2% respectively between 2 Guangzhou isolates and African isolate.


CONCLUSIONS
HGV infection exists in hepatitis patients, 2 Guangzhou HGV isolates may belong to the same gene type and both of them have a high homology with American HGV isolate.
Tang
Y
Y
Institute of Infectious Diseases, Eighth People's Hospital of Guangzhou, Guangzhou 510060, China. yangbotang@163.net
Li
D
D
Liu
G
G
D001483:Base Sequence; D004279:DNA, Viral / Q000097:blood; D018178:Flaviviridae Infections / Q000235:genetics / Q000821:virology*; D029407:GB virus C / Q000235:genetics / Q000302:isolation & purification; D006509:Hepatitis B / Q000821:virology; D006526:Hepatitis C / Q000821:virology; D006525:Hepatitis, Viral, Human / Q000235:genetics / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D004279:DNA, Viral