[Observation of cell ultrastructuse in suppurative otitis media treated with bosneol and application].
OBJECTIVE
To explore the pharmacological action and optimal concentration of borneol in treating suppurative otitis media.


METHODS
Aspergillus niger separated from the secretion in external auditory canal of the patient and the fungus strains kept in the laboratory were used to determine the minimum fungus inhibiting concentration (MIC) and minimum fungus killing concentration (MFC) of borne. The change of cell ultrastructure after borneol taking effect was observed under electron microscope.


RESULTS
Borneol MIC and MFC turned out to be 5% and 10% respectively. The cell wall of Aspergillus niger became thicker and blurred with vacuoles. In the cytoplasm various materials were found deep dyed and agglutinated with many fat drops. The electron density increased and the ridge arrangement was in disorder. Some organelles were destroyed, and many areas of electron blank appeared. Some cells became warped and deformed and lost their original structure.


CONCLUSIONS
Borneol can destroy the structure of fungus cells, make fungus dissolve and dieout and thus features a fungus inhibiting and killing function. The optimal inhibiting concentration is 5% and killing concentration 10%.
Chang
S P
SP
Haerbin Moslem Hospital, Haerbin 150001, Heilongjiang, China.
Li
Y C
YC
D000935:Antifungal Agents / Q000627:therapeutic use*; D001228:Aspergillosis / Q000188:drug therapy*; D001234:Aspergillus niger / Q000187:drug effects* / Q000302:isolation & purification / Q000648:ultrastructure; D001892:Camphanes / Q000627:therapeutic use*; D006801:Humans; D008419:Materia Medica / Q000627:therapeutic use*; D010035:Otitis Media, Suppurative / Q000188:drug therapy* / Q000382:microbiology
D000935:Antifungal Agents; D001892:Camphanes; D008419:Materia Medica; C022871:isoborneol