title: Use of large particles support for fast analysis of methadone and its primary metabolite in human plasma by liquid chromatography-mass spectrometry.
abstract: A bioanalytical method was developed for the quantitation of methadone (MTD) and its primary metabolite, (EDDP) in plasma. The extraction step was performed within a capillary column packed with large particles (35x0.3 mm I.D.; d(p) 30 micrometer) at high flow-rate conditions (450 microliter/min). The separation was performed on a microbore analytical column (55x2 mm I.D.; d(p) 3 micrometer) coupled to a mass spectrometer (MS). This procedure was based on a column-switching unit. Analytes of interest were retained on the precolumn by hydrophobic interactions and backflushed from the precolumn to the analytical column. The detection was carried out with a MS single quadrupole equipped with an electrospray interface. The total analysis time was 6 min. The limits of quantification were evaluated at 10 and 25 ng/ml for MTD and EDDP, respectively. At this level, good accuracies were obtained for both analytes with repeatability values less than 18%.
authors.affiliation: Laboratory of Analytical Pharmaceutical Chemistry, University of Geneva, 20 Boulevard d'Yvoy, 1211 4, Geneva, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Souverain
authors.forename: S
authors.initials: S
authors.lastname: Rudaz
authors.forename: D
authors.initials: D
authors.lastname: Ortelli
authors.forename: E
authors.initials: E
authors.lastname: Varesio
authors.forename: J L
authors.initials: JL
authors.lastname: Veuthey
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008691:Methadone / Q000097:blood*
chemical_list: D008691:Methadone