title: Novel surface modification of polymer-based separation media controlling separation selectivity, retentivity and generation of electroosmotic flow.
abstract: Uniformly sized packing materials based on synthetic polymer particles for high-performance liquid chromatography (HPLC) and capillary electrochromatography (CEC) have been prepared from polymerization mixtures containing methacrylic acid (MAA) as a functional monomer and by using a novel surface modification method. This "dispersion method" affords effectively modified separation media. Both the amount of MAA utilized in the preparation and reaction time affect the selectivity of chromatographic separation in both the HPLC and the CEC mode and electroosmotic flow. This detailed study revealed that the dispersion method effectively modified internal surface of macroporous separation media and, based on the amount of MAA introduced, exclusion mechanism for the separation of certain solutes could be observed.
authors.affiliation: Department of Polymer Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. kenpc@ipc.kit.ac.jp
authors.forename: Ken
authors.initials: K
authors.lastname: Hosoya
authors.forename: Takuya
authors.initials: T
authors.lastname: Kubo
authors.forename: Katsuo
authors.initials: K
authors.lastname: Takahashi
authors.forename: Tohru
authors.initials: T
authors.lastname: Ikegami
authors.forename: Nobuo
authors.initials: N
authors.lastname: Tanaka
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation*; D009995:Osmosis; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D011108:Polymers