Microwave irradiation for rapid epidermis-dermis separation and improved epidermal cell immunodetection.
We explored the effects of microwave irradiation on epidermal-dermal separation and subsequent immunostaining of epidermal cells. Epidermal sheets were obtained after incubation in 0.02 M EDTA in PBS and microwave irradiation with 4 pulses of 420 watts for 5 sec, with a total incubation period of 4 min. The control epidermal sheets were immunostained for Langerhans cells and dendritic epidermal T cells using a conventional immunoperoxidase method. The experimental immunodetection of these cells was assisted by incubating the primary antibodies for 10 min at 70 watts. We showed a simple and rapid method for separation of the epidermal-dermal junction and immunostaining of epidermal cells with optimal morphological preservation.
Sánchez
M A
MA
Instituto de Biomedicina, Universidad Central de Venezuela, Apartado 4043 Caracas 1010A, Venezuela.
Díaz
N L
NL
Tapia
F J
FJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D002469:Cell Separation / Q000379:methods; D003713:Dendritic Cells / Q000378:metabolism; D020405:Dermis / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D000078404:Epidermal Cells; D004817:Epidermis / Q000528:radiation effects*; D019084:Fluorescent Antibody Technique, Indirect; D007150:Immunohistochemistry; D007801:Langerhans Cells / Q000378:metabolism; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008872:Microwaves; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C093569:DEC-205 receptor; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface