title: Accuracy of a commercial enzyme-linked immunosorbent assay for CagA in patients from Brazil with and without gastric carcinoma.
abstract: We validated a commercial enzyme-linked immunosorbent assay for the detection of anti-CagA antibodies in Brazilian patients with Helicobacter pylori infection. The test presented high sensitivity (97.4%) and specificity (88.9%) when employed in patients without gastric carcinoma. However, in gastric carcinoma patients, the test was neither sensitive nor specific enough to detect cagA-positive H. pylori infection.
authors.affiliation: Laboratory of Research in Bacteriology, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Andreia Maria Camargos
authors.initials: AM
authors.lastname: Rocha
authors.forename: Gifone Aguiar
authors.initials: GA
authors.lastname: Rocha
authors.forename: Adriana
authors.initials: A
authors.lastname: Santos
authors.forename: Celso Affonso
authors.initials: CA
authors.lastname: de Oliveira
authors.forename: Dulciene Maria Magalhães
authors.initials: DM
authors.lastname: Queiroz
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis*; D001938:Brazil; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D016481:Helicobacter Infections / Q000150:complications / Q000276:immunology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D011933:Reagent Kits, Diagnostic; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000150:complications / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011933:Reagent Kits, Diagnostic; C080866:cagA protein, Helicobacter pylori