[Various effects of laminin-1 and laminin-2/4 on adhesion and migration of cultured human keratinocytes].
The cell-matrix interaction is one of the factors defining the cell behavior in normal and wounded tissues. To determine the function of laminin-2/4, one of components of the skin basement membrane in the process of reepithelization, we studied its interaction with human keratinocytes. The adhesive properties of laminin-2/4 and its effect on keratinocytes migration in vitro were analysed. For comparison with our present investigation, we used the earlier studied laminin-1 from EHS mouse sarcoma. Laminin-2/4 appeared to be a good substrate for human keratinocytes, and this correlates with a greater number of cell surface receptors compared with laminin-1. Laminin-2/4 alone does not stimulate keratinocyte migration, but, in contrast to laminin-1, supports EGF-mediated migration. The obtained results give an insight into the function of laminin-2/4 in normal skin and during wound healing.
Kalmykova
N V
NV
Institute of Cytology RAS, St. Petersburg. kanv@mailru.com
Cherepanova
O A
OA
Gorelik
Iu V
IuV
Voronkina
I V
IV
Blinova
M I
MI
Pinaev
G P
GP
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000502:physiology*; D007797:Laminin / Q000494:pharmacology / Q000502:physiology*; D051379:Mice; D020033:Protein Isoforms
C501369:LAMA4 protein, human; C501370:Lama4 protein, mouse; D007797:Laminin; D020033:Protein Isoforms; C121346:laminin 1; C102645:laminin alpha 2; D004815:Epidermal Growth Factor