[Interaction of membrane proton conductivity, membrane and oxidation-reduction potential in Escherichia coli].
It was shown that the proton conductivity of Escherichia coli membranes depends on pH and other conditions of bacterial growth. It is considerably lower in cells fermenting glucose and accomplishing the nitrate-nitrite respiration compared with cells accomplishing the oxygen respiration. Proton conductivity increases substantially with decreasing pH of medium. It was found that proton conductivity is related to the redox and membrane potentials of cells. The energy-dependent flux of protons from cells and the ATPase activity of membrane vesicles considerably vary depending on whether bacteria are grown under aerobic or anaerobic conditions. The H+ flux from cells fermenting glucose (pH 7.5) was 1.7 times greater than the H+ flux from cells that accomplish the nitrate-nitrite and oxygen respiration. The N,N'-dicyclohexylcarbodiimide (DCCD)-sensitive ATPase activity increased 2.5 times as K+ concentration increased to 100 mM (including residual K+ in potassium-free medium). The DCCD-sensitive ATPase activity considerably decreased with decreasing pH of medium, whereas the ATPase activity that was not suppressed by DCCD was stimulated. These results can be used for establishing the relationship between membrane proton conductivity and the energy-dependent H+ flux and ATPase activity.
Akopian
K
K
Yerevan State University, ul. A. Manukyana 1, Yerevan, 375026 Armenia.
Zakharian
E
E
Kirakosian
G
G
Mnatsakanian
N
N
Bagramian
K
K
Trchunian
A
A
D000251:Adenosine Triphosphatases / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology / Q000502:physiology; D004553:Electric Conductivity; D004926:Escherichia coli / Q000502:physiology* / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials; D010084:Oxidation-Reduction; D011522:Protons
D011522:Protons; D000251:Adenosine Triphosphatases