The role of focal adhesion kinase in the emigration of cells from confluent cultures.
We studied the effect of the modification of focal adhesion kinase (FAK) on the growth, migration and adhesion of C3H 10T1/2 cells. Cells transfected with plasmid coding for antisense FAK displayed a low level of FAK protein. Interestingly, the transfected cells achieved a higher saturation density at confluence, and displayed reduced adhesion and enhanced emigration from a confluent layer of cells when stimulated with fibronectin. In conclusion, it can be postulated that FAK plays an important role in the mechanism of contact inhibition.
Trembacz
Halina
H
Department of Cell Biology, Cancer Center, M. Skłodowska-Curie Institute of Oncology, W.K. Roentgen 5, 02-781Warsaw, Poland.
Miłoszewska
Joanna
J
Szaniawska
Bozena
B
Małecki
Maciej
M
Przybyszewska
Małgorzata
M
Skorski
Tomasz
T
Janik
Przemysław
P
D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D016373:DNA, Antisense; D018076:DNA, Complementary / Q000378:metabolism*; D015536:Down-Regulation; D005353:Fibronectins / Q000378:metabolism; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; D051379:Mice; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*
D016373:DNA, Antisense; D018076:DNA, Complementary; D005353:Fibronectins; D011505:Protein-Tyrosine Kinases; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; C496051:Ptk2 protein, mouse