Crystal structure of triosephosphate isomerase complexed with 2-phosphoglycolate at 0.83-A resolution.
The atomic resolution structure of Leishmania mexicana triosephosphate isomerase complexed with 2-phosphoglycolate shows that this transition state analogue is bound in two conformations. Also for the side chain of the catalytic glutamate, Glu(167), two conformations are observed. In both conformations, a very short hydrogen bond exists between the carboxylate group of the ligand and the catalytic glutamate. The distance between O11 of PGA and Oepsilon2 of Glu(167) is 2.61 and 2.55 A for the major and minor conformations, respectively. In either conformation, Oepsilon1 of Glu(167) is hydrogen-bonded to a water network connecting the side chain with bulk solvent. This network also occurs in two mutually exclusive arrangements. Despite the structural disorder in the active site, the C termini of the beta strands that construct the active site display the least anisotropy compared with the rest of the protein. The loops following these beta strands display various degrees of anisotropy, with the tip of the dimer interface loop 3 having very low anisotropy and the C-terminal region of the active site loop 6 having the highest anisotropy. The pyrrolidine ring of Pro(168) at the N-terminal region of loop 6 is in a strained planar conformation to facilitate loop opening and product release.
Kursula
Inari
I
Department of Biochemistry and Biocenter Oulu, University of Oulu, P. O. Box 3000, Oulu FIN-90014, Finland.
Wierenga
Rik K
RK
D000818:Animals; D016880:Anisotropy; D001665:Binding Sites; D018360:Crystallography, X-Ray; D019281:Dimerization; D018698:Glutamic Acid / Q000737:chemistry; D006016:Glycolates / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry; D006860:Hydrogen Bonding; D007894:Leishmania mexicana / Q000201:enzymology; D008956:Models, Chemical; D008958:Models, Molecular; D011392:Proline / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011522:Protons; D014305:Triose-Phosphate Isomerase / Q000737:chemistry*
D006016:Glycolates; D011522:Protons; D018698:Glutamic Acid; D006639:Histidine; D011392:Proline; D014305:Triose-Phosphate Isomerase; C013193:phosphoglycolate