title: Mcm1p-induced DNA bending regulates the formation of ternary transcription factor complexes.
abstract: The yeast MADS-box transcription factor Mcm1p plays an important regulatory role in several diverse cellular processes. In common with a subset of other MADS-box transcription factors, Mcm1p elicits substantial DNA bending. However, the role of protein-induced bending by MADS-box proteins in eukaryotic gene regulation is not understood. Here, we demonstrate an important role for Mcm1p-mediated DNA bending in determining local promoter architecture and permitting the formation of ternary transcription factor complexes. We constructed mutant mcm1 alleles that are defective in protein-induced bending. Defects in nuclear division, cell growth or viability, transcription, and gene expression were observed in these mutants. We identified one likely cause of the cell growth defects as the aberrant formation of the cell cycle-regulatory Fkh2p-Mcm1p complex. Microarray analysis confirmed the importance of Mcm1p-mediated DNA bending in maintaining correct gene expression profiles and revealed defects in Mcm1p-mediated repression of Ty elements and in the expression of the cell cycle-regulated YFR and CHS1 genes. Thus, we discovered an important role for DNA bending by MADS-box proteins in the formation and function of eukaryotic transcription factor complexes.
authors.affiliation: Department of Biochemistry and Genetics, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom.
authors.forename: Fei-Ling
authors.initials: FL
authors.lastname: Lim
authors.forename: Andrew
authors.initials: A
authors.lastname: Hayes
authors.forename: Adam G
authors.initials: AG
authors.lastname: West
authors.forename: Aline
authors.initials: A
authors.lastname: Pic-Taylor
authors.forename: Zoulfia
authors.initials: Z
authors.lastname: Darieva
authors.forename: Brian A
authors.initials: BA
authors.lastname: Morgan
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Oliver
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Sharrocks
mesh_terms: D000483:Alleles; D001483:Base Sequence; D001665:Binding Sites; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D017930:Genes, Reporter; D005982:Glutathione Transferase / Q000378:metabolism; D026341:Minichromosome Maintenance 1 Protein / Q000235:genetics* / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009690:Nucleic Acid Conformation; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D012689:Sequence Homology, Nucleic Acid; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; D026341:Minichromosome Maintenance 1 Protein; D004247:DNA; D005982:Glutathione Transferase