[Application of nitrocellulose membrane in cell culture and pathological techniques].
OBJECTIVE
To provide a new supporting medium for carrying out cell culture and histological staining.


METHODS
LoVo cell line was cultured using nitrocellulose membrane as the culture medium, in parallel with the same cell culture on the coverglass which served as control.


RESULTS
The cells cultured on nitrocellulose membrane showed no visible difference from those cultured on coverglasses in terms of cell growth, morphology, and the structure displayed after staining. No toxic effect was observed due to the application of nitrocellulose membrane.


CONCLUSIONS
Nitrocellulose membrane after treatment has such merit as good lucidity and stability without toxicity on the cells. Having also good affinity with the cells, nitrocellulose membrane can offer an promising alternative for glass as cell culture medium.
Zhang
Hong
H
Surgical Laboratory, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Wang
Ya-dong
YD
Lai
Zhuo-sheng
ZS
D018929:Cell Culture Techniques / Q000379:methods*; D003101:Collodion; D006801:Humans; D013194:Staining and Labeling / Q000379:methods*; D014407:Tumor Cells, Cultured
D003101:Collodion