title: Thioridazine induces erythrocyte stomatocytosis due to interactions with negatively charged lipids.
abstract: Despite the fact that thioridazine is used clinically as a neuroleptic drug, little is known about the molecular mechanisms underlying its biological effects, in particular about its interactions with membranes. In the present work we investigate the influence of thioridazine on model and cell membranes, using calorimetry, DPH fluorescence polarization measurements, studies of haemolysis and scanning electron microscopy. The experiments show that thioridazine interacts with lipid bilayers and intercalates into bilayer structure. We found that erythrocyte stomatocytosis induced by the drug might be related to preferential interaction of thioridazine with charged lipids.
authors.affiliation: Department of Biophysics, Wrocław Medical University, Chałubińskiego 10, 50-368 Wrocław, Poland.
authors.forename: Andrzej B
authors.initials: AB
authors.lastname: Hendrich
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Lichacz
authors.forename: Anna
authors.initials: A
authors.lastname: Burek
authors.forename: Krystyna
authors.initials: K
authors.lastname: Michalak
mesh_terms: D014150:Antipsychotic Agents / Q000494:pharmacology*; D002151:Calorimetry; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005454:Fluorescence Polarization; D006801:Humans; D050356:Lipid Metabolism; D008855:Microscopy, Electron, Scanning; D013881:Thioridazine / Q000494:pharmacology*
chemical_list: D014150:Antipsychotic Agents; D013881:Thioridazine