title: Expression of protein phosphatase 1 during the asexual development of Neurospora crassa.
abstract: We cloned and sequenced the cDNA and the gene encoding the catalytic subunit of protein phosphatase 1 from the filamentous fungus Neurospora crassa. The gene, designated ppp-1 (phosphoprotein phosphatase 1), was mapped by restriction fragment length polymorphism to linkage group III, in the vicinity of con-7 and trp-1. The expression of the gene was monitored by reverse transcriptase and polymerase chain reactions, by Western blotting, and by protein phosphatase activity assays in synchronized cultures. Transcripts of ppp-1 were detected in the dormant conidia. The abundance of ppp-1 mRNA, Ppp-1 protein, and the activity of protein phosphatase 1 increased during germination and subsequent hyphal elongation as well as during the early stages of aerial mycelium formation.
authors.affiliation: Department of Medical Chemistry, Medical and Health Sciences Center, University of Debrecen, H-4026 Debrecen, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Zeke
authors.forename: Endre
authors.initials: E
authors.lastname: Kókai
authors.forename: Balázs
authors.initials: B
authors.lastname: Szöor
authors.forename: Einat
authors.initials: E
authors.lastname: Yatzkan
authors.forename: Oded
authors.initials: O
authors.lastname: Yarden
authors.forename: Krisztina
authors.initials: K
authors.lastname: Szirák
authors.forename: Zsigmond
authors.initials: Z
authors.lastname: Fehér
authors.forename: Péter
authors.initials: P
authors.lastname: Bagossi
authors.forename: Pál
authors.initials: P
authors.lastname: Gergely
authors.forename: Viktor
authors.initials: V
authors.lastname: Dombrádi
mesh_terms: D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D015723:Gene Library; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000201:enzymology*; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis* / Q000737:chemistry*; D010766:Phosphorylation; D012150:Polymorphism, Restriction Fragment Length; D014176:Protein Biosynthesis; D054645:Protein Phosphatase 1; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013997:Time Factors
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1