[Frequency of genes speA, speB, and speC in Streptococcus pyogenes strains and the identification of the infective agent by polymerase chain reaction].
In cultures of S. pyogenes isolated from patients and carriers in different territories of the Russian Federation the genes of erythorogenic toxins A, B and C (speA, speB and specC) were detected. The possibility of the identification of S. pyogenes by means of PCR on the basis of primers to erythrogenic toxin B was determined. Gene speB was detected in all S. pyogenes cultures under study and proved to be species specific. Genes speA and speC were detected, respectively, in 29.4% and 9.35% of the S. pyogenes cultures under study. A test system for the identification of S. pyogenes on the basis of primers to gene speB was developed. The prospects for the detection of genes speA and speC for intraspecific typing of this infective agent were evaluated.
Dmitrieva
N F
NF
Sechenov Medical Academy, Moscow, Russia.
Trofimov
D Iu
DIu
Eshchina
A S
AS
Riapis
L A
LA
Pavlova
O G
OG
Petrova
T V
TV
Skorkina
Iu A
IuA
Gerasimov
A N
AN
Alekseev
L P
LP
Zhuravlev
M V
MV
Briko
N I
NI
D001426:Bacterial Proteins; D017931:DNA Primers; D005098:Exotoxins / Q000032:analysis / Q000235:genetics*; D005787:Gene Frequency; D005798:Genes, Bacterial; D006801:Humans; D008565:Membrane Proteins; D016133:Polymerase Chain Reaction / Q000379:methods; D012426:Russia / Q000453:epidemiology; D013045:Species Specificity; D013290:Streptococcal Infections / Q000453:epidemiology / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000235:genetics* / Q000302:isolation & purification*
D001426:Bacterial Proteins; D017931:DNA Primers; D005098:Exotoxins; D008565:Membrane Proteins; C117980:SpeA protein, Streptococcus pyogenes; C027951:erythrogenic toxin