[Pilot experience of studying calicivirus infection in Moscow children].
An RT-PCR-based assay for detecting calicivirus RNA was developed. Samples of feces of 124 children patients with acute gastroenteritis were tested for the presence of NLV-1, NLV-2, and SLV. Calicivirus infection was detected in 6.4% of cases of children with acute gastroenteritis. All isolates were non-homogeneous Norwalk like Viruses 2 genotype with maximum nucleotide homology to the strains, isolated in UK and Japan. The use of the RT-PCR assay developed in our laboratory allowed the etiology of 22.2% of cases of acute gastroenteritis of uncertain etiology to be identified.
Mukhina
A A
AA
Shipulin
G A
GA
Bokovoĭ
A G
AG
Iatsyshina
S B
SB
D000208:Acute Disease; D017250:Caliciviridae Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000209:etiology; D002675:Child, Preschool; D017931:DNA Primers; D005243:Feces / Q000821:virology*; D005759:Gastroenteritis / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D007223:Infant; D009031:Moscow / Q000453:epidemiology; D029322:Norovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D019590:Nucleocapsid Proteins / Q000235:genetics; D010802:Phylogeny; D010865:Pilot Projects; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029341:Sapovirus / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D019590:Nucleocapsid Proteins; D012367:RNA, Viral