Effective transfection of a cis element "decoy" of the nuclear factor-kappaB binding site into the experimental choroidal neovascularization.
OBJECTIVE
To evaluate the efficacy of the gene transfer of a double-stranded phosphorothioate oligonucleotides (ODNs), called a "decoy", against the NF-kappaB binding site into cells of an experimentally-induced choroidal neovascularization.


METHODS
FITC-labeled decoy was injected into the subretinal space of rat eyes by the HVJ-liposome delivery system, and 3 days later, choroidal neovascularization was induced by laser photocoagulation. The eyes were removed and the transfected cells were detected by fluorescence microscopy and also detected by immunohistochemistry. The degree of neovascularization was evaluated by fluorescein angiography.


RESULTS
The decoy was transfected into the retinal pigment epithelial (RPE) cells, inner and outer segment of the photoreceptors at 3 days after the injection. When choroidal neovascularization was induced, highly effective transfection of the decoy was observed 3 to 14 days after photocoagulation, after which the level decreased. Decoys were transfected into the RPE cells and macrophages in the choroidal neovascularization. The eyes transfected with NF-kappaB decoy showed a weaker leakage in fluorescein angiograms than that of the control eyes transfected with scrambled decoy.


CONCLUSIONS
A decoy can be transfected into retinal cells and cells within a choroidal neovascularization by the HVJ-liposome method. The transferred NF-kappaB decoy reduced the degree of choroidal neovascularization. Decoy targeted against NF-kappaB may be considered as a potential therapy for neovascularization.
Jo
Nobuo
N
Department of Ophthalmology, Kansai Medical University, Moriguchi, Osaka, Japan.
Ogata
Nahoko
N
Aoki
Motokuni
M
Otsuji
Tsuyoshi
T
Morishita
Ryuichi
R
Kaneda
Yasufumi
Y
Matsumura
Miyo
M
D000818:Animals; D001665:Binding Sites / Q000235:genetics; D020256:Choroidal Neovascularization / Q000473:pathology / Q000628:therapy*; D005451:Fluorescein Angiography; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D015316:Genetic Therapy; D007150:Immunohistochemistry; D008081:Liposomes; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D011914:Rats, Inbred BN; D013237:Stereoisomerism; D014162:Transfection
D005456:Fluorescent Dyes; D008081:Liposomes; D016328:NF-kappa B; D016650:Fluorescein-5-isothiocyanate