title: Fibroblast and epidermal growth factors modulate proliferation and neural cell adhesion molecule expression in epithelial cells derived from the adult mouse tongue.
abstract: Lingual epithelial cells, including those of the taste buds, are regularly replaced by proliferative stem cells. We found that integrin beta(1), a keratinocyte stem cell marker, was expressed at the basal layer and taste buds of adult mouse tongue epithelium. We purified and cultured integrin beta(1)-positive cells (termed KT-1 cells), whose growth was stimulated by epidermal growth factor (EGF) and basic fibroblast growth factor (FGF-2). FGF-2 stimulation induced translocation of the FGF type I receptor (FGFR1) into nuclei, suggesting that the growth-stimulating effect of FGF-2 was mediated through FGFR1. EGF and FGF-2 also regulated cell surface expression of the neural cell adhesion molecule (N-CAM) in KT-1 cells. Anti-N-CAM antibody immunoreactivity was restricted to the gustatory epithelium and the nerves in the tongue epithelium, giving rise to the possibility that KT-1 may contain gustatory epithelial cells. KT-1 cells may thus be useful for analyzing the factors that regulate the growth and differentiation of lingual and gustatory epithelial cells in vitro.
authors.affiliation: National Food Research Institute, 2-1-12 Kan-nondai, Tsukuba, Ibaraki 305-8642, Japan. ookura@nfri.affrc.go.jp
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ookura
authors.forename: Keiko
authors.initials: K
authors.lastname: Kawamoto
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Tsuzaki
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Mikami
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Ito
authors.forename: Seong-Lee
authors.initials: SL
authors.lastname: Oh
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hino
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D018929:Cell Culture Techniques; D002455:Cell Division / Q000502:physiology; D004847:Epithelial Cells / Q000378:metabolism / Q000502:physiology*; D066246:ErbB Receptors / Q000502:physiology*; D005786:Gene Expression Regulation; D019012:Integrin beta1 / Q000502:physiology; D051379:Mice; D017468:Receptors, Fibroblast Growth Factor / Q000502:physiology*; D013650:Taste Buds / Q000737:chemistry / Q000196:embryology / Q000254:growth & development; D014059:Tongue / Q000166:cytology* / Q000294:innervation
chemical_list: D000943:Antigens, Differentiation; D015816:Cell Adhesion Molecules, Neuronal; D019012:Integrin beta1; D017468:Receptors, Fibroblast Growth Factor; D066246:ErbB Receptors