title: Electrochemical and Raman studies of the biointeraction between Escherichia coli and mannose in polydiacetylene derivative supported on the self-assembled monolayers of octadecanethiol on a gold electrode.
abstract: Here, we describe a new method to study the biointeraction between Escherichia coli and mannose by using supramolecular assemblies composed of polydiacetylene supported on the self-assembled monolayer of octadecanethiol on a gold electrode. These prepared bilayer materials simply are an excellent protosystem to study a range of important sensor-related issues. The experimental results from UV-vis spectroscopy, resonance Raman spectroscopy, and electrochemistry confirm that the specific interactions between E. coli and mannose can cause conformational changes of the polydiacetylene backbone rather than simple nonspecific adsorption. Moreover, the direct electrochemical detection by polydiacetylene supramolecular assemblies not only opens a new path for the use of these membranes in the area of biosensor development but also offers new possibilities for diagnostic applications and screening for binding ligands.
authors.affiliation: Laboratory of Excited-State Process, Changchun Institute of Optic Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130021, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Li
authors.forename: Baoliang
authors.initials: B
authors.lastname: Ma
authors.forename: Yi
authors.initials: Y
authors.lastname: Fan
authors.forename: Xianggui
authors.initials: X
authors.lastname: Kong
authors.forename: Jinghong
authors.initials: J
authors.lastname: Li
mesh_terms: D000114:Acetylene / Q000031:analogs & derivatives* / Q000737:chemistry*; D000327:Adsorption; D004563:Electrochemistry; D004566:Electrodes; D004926:Escherichia coli / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D046911:Macromolecular Substances; D008358:Mannose / Q000378:metabolism*; D015394:Molecular Structure; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes; D013059:Spectrum Analysis, Raman; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D046911:Macromolecular Substances; D000078789:Polyacetylene Polymer; D011108:Polymers; D013438:Sulfhydryl Compounds; D053279:Polyynes; C082361:polydiacetylene; C055360:n-octadecyl mercaptan; D006046:Gold; D000114:Acetylene; D008358:Mannose