Lysis of methicillin-resistant Staphylococcus aureus by 2,4-diacetylphloroglucinol produced by Pseudomonas sp. AMSN isolated from a marine alga.
Previously 2,4-diacetylphloroglucinol (DAPG) produced by Pseudomonas sp. AMSN isolated from a marine alga, had demonstrated a high level of anti-methicillin-resistant Staphylococcus aureus (MRSA) comparable with that of vancomycin. In this study, this substance had bacteriolytic activity against MRSA at 1 microg/ml as well as similar activity against Vibrio parahaemolyticus at 24 microg/ml that suggests a novel antibacterial mode of action by this substance. Heat and pH stability tests showed it to be stable at temperatures ranging from 35 to 70 degrees C and at pHs ranging from 2-7. It was not acutely toxic to mice at levels up to 100 mg/kg.
Kamei
Yuto
Y
Marine and Highland Bioscience Center, Saga University, 152-1 Shonan-cho, Karatsu, Saga 847-0021, Japan. kameiy@cc.saga-u.ac.jp
Isnansetyo
Alim
A
D001433:Bacteriolysis / Q000187:drug effects*; D056890:Eukaryota / Q000382:microbiology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000494:pharmacology*; D011549:Pseudomonas / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000187:drug effects*
C059817:2,4-diacetylphloroglucinol; D010696:Phloroglucinol