NESbase version 1.0: a database of nuclear export signals.
Protein export from the nucleus is often mediated by a Leucine-rich Nuclear Export Signal (NES). NESbase is a database of experimentally validated Leucine-rich NESs curated from literature. These signals are not annotated in databases such as SWISS-PROT, PIR or PROSITE. Each NESbase entry contains information of whether NES was shown to be necessary and/or sufficient for export, and whether the export was shown to be mediated by the export receptor CRM1. The compiled information was used to make a sequence logo of the Leucine-rich NESs, displaying the conservation of amino acids within a window of 25 residues. Surprisingly, only 36% of the sequences used for the logo fit the widely accepted NES consensus L-x(2,3)-[LIVFM]-x(2,3)-L-x-[LI]. The database is available online at http://www.cbs.dtu.dk/databases/NESbase/.
la Cour
Tanja
T
Center for Biological Sequence Analysis, Building-208, Technical University of Denmark, DK-2800 Lyngby, Denmark.
Gupta
Ramneek
R
Rapacki
Kristoffer
K
Skriver
Karen
K
Poulsen
Flemming M
FM
Brunak
Søren
S
D021581:Active Transport, Cell Nucleus; D020816:Amino Acid Motifs; D000818:Animals; D017124:Conserved Sequence; D030562:Databases, Protein; D007930:Leucine / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D016415:Sequence Alignment
D011506:Proteins; D007930:Leucine