title: [Biological analysis of a novel gene related with interleukin-6].
abstract: OBJECTIVE
  To understand the action mechanism of interleukin (IL)-6 through investigation of its related genes.
  
  
  METHODS
  Using electronic cloning with IL-6-related expressed sequence tags (ESTs), a novel gene at its full length of 924 bp was acquired. Reverse transcriptase-PCR method was subsequently employed to amplify the cDNA of this gene from the total RNA of human U937 cells, which were previously activated with 100 ng/ml IL-6 for 8 h. The fragment was then ligated into the pGEM-Teasy vector and sequence analysis was conducted.
  
  
  RESULTS
  The whole cDNA gene was successfully fished.
  
  
  CONCLUSIONS
  Electronic cloning technology can be instrumental in identifying novel genes.
authors.affiliation: Deparment of Transfusion, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xia
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Lun-jiang
authors.initials: LJ
authors.lastname: Lin
authors.forename: Jian-nan
authors.initials: JN
authors.lastname: Feng
authors.forename: Jiong-cai
authors.initials: JC
authors.lastname: Lan
authors.forename: Bei-fen
authors.initials: BF
authors.lastname: Shen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification*; D020224:Expressed Sequence Tags; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020298:U937 Cells
chemical_list: D018076:DNA, Complementary; D015850:Interleukin-6