Structural characterisation of a perdeuteriomethylated exopolysaccharide by NMR spectroscopy: characterisation of the novel exopolysaccharide produced by Lactobacillus delbrueckii subsp. bulgaricus EU23.
The exopolysaccharide (EPS) from Lactobacillus delbrueckii subsp. bulgaricus EU23 was perdeuteriomethylated and the perdeuteriomethylated EPS (pdm-EPS) purified by elution from a C(18) Sep-Pak cartridge. Both 1D and 2D NMR spectra were recorded for the pdm-EPS and these were interpreted to provide assignments for the individual 1H and 13C resonances of the sugar residues of the repeating unit. Using a combination of the results from monomer analysis and linkage analysis of the native EPS and the ROESY and HMBC NMR spectra of the pdm-EPS the following structure has been determined for the repeating unit:A process for characterising polysaccharides having low solubility in aqueous solution is reported.
Harding
Lindsay P
LP
School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield HD1 3DH, UK.
Marshall
Valerie M
VM
Elvin
Mark
M
Gu
Yucheng
Y
Laws
Andrew P
AP
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003903:Deuterium; D007778:Lactobacillus / Q000737:chemistry*; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D011135:Polysaccharides, Bacterial; D003903:Deuterium