title: Interleukin regulation of asymmetric antibody synthesized by isolated placental B cells.
abstract: OBJECTIVE
  Protecting antibodies against trophoblast surface molecules were previously described. Here we analysed the synthesis of asymmetric IgG by placental B-lymphocytes.
  
  
  METHODS
  B cells were isolated from human term placenta and cord blood, stimulated with anti-CD40 IgG and cocultured with transfected Fcgamma R-expressing mice Ltk-fibroblast. Interleukin-4, IL-6, IL-10, IL-11 and IL-13 were added to cultures for 14 days. Asymmetric IgG were assessed in culture supernatants by concanavalin A (Con A) fixation and enzyme-linked immunosorbent assay.
  
  
  RESULTS
  When IL-6 was added to the cultures, the percentages of asymmetric IgG synthesized by placental B cells were: IL-6: 29 +/- 10; IL-6 + IL-10: 24 +/- 7; IL-4 + IL-10 + IL-6: 38 +/- 9. The last combination induced the highest increase in the asymmetric IgG synthesis as compared with control (19 +/- 10%, P < 0.05). Additionally, placental B cells synthesized more asymmetric IgG than umbilical cord blood B-lymphocytes (P = 0.0015).
  
  
  CONCLUSIONS
  Isolated placental B-lymphocytes synthesized asymmetric IgG in response to Th2 interleukins, more notably IL-6 in combination with IL-4 and IL-10. The in vitro increase of protective asymmetric IgG synthesis in response to Th2-cytokines support the hypothesis that a local Th2-switch is beneficial for pregnancy outcome.
authors.affiliation: IDEHU-National Council of Scientific and Technological Research, University of Buenos Aires, Buenos Aires, Argentina. acanell@ffyb.uba.ar
authors.forename: A
authors.initials: A
authors.lastname: Canellada
authors.forename: A
authors.initials: A
authors.lastname: Färber
authors.forename: A C
authors.initials: AC
authors.lastname: Zenclussen
authors.forename: T
authors.initials: T
authors.lastname: Gentile
authors.forename: J
authors.initials: J
authors.lastname: Dokmetjian
authors.forename: A
authors.initials: A
authors.lastname: Keil
authors.forename: S
authors.initials: S
authors.lastname: Blois
authors.forename: S
authors.initials: S
authors.lastname: Miranda
authors.forename: L
authors.initials: L
authors.lastname: Berod
authors.forename: G
authors.initials: G
authors.lastname: Gutiérrez
authors.forename: U R
authors.initials: UR
authors.lastname: Markert
authors.forename: R A
authors.initials: RA
authors.lastname: Margni
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007378:Interleukins / Q000378:metabolism*; D051379:Mice; D010920:Placenta / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D007378:Interleukins