title: Structural features of the glycogen branching enzyme encoding genes from aspergilli.
abstract: A maltose binding protein, p78, was purified to homogeneity from Aspergillus nidulans by a single column chromatography step on cross-linked amylose. The partial amino acid sequence was highly homologous to the glycogen branching enzymes (GBEs) of human and yeast, and p78 did show branching enzyme activity. The genomic gene and its cDNA encoding GBE (p78) were isolated from the A. nidulans genomic and cDNA libraries. Furthermore, a cDNA encoding A. oryzae GBE was entirely sequenced. A. nidulans GBE shared overall and significant amino acid sequence identity with GBEs from A. oryzae (83.9%), Saccharomyces cerevisiae (61.1%) and human (63.0%), and with starch branching enzymes from green plants (55-56%).
authors.affiliation: Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya-shi, Aichi 464-8601, Japan.
authors.forename: Prasetyawan
authors.initials: P
authors.lastname: Sasangka
authors.forename: Aya
authors.initials: A
authors.lastname: Matsuno
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Tanaka
authors.forename: Yuki
authors.initials: Y
authors.lastname: Akasaka
authors.forename: Sachie
authors.initials: S
authors.lastname: Suyama
authors.forename: Sumie
authors.initials: S
authors.lastname: Kano
authors.forename: Makiko
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Akao
authors.forename: Masashi
authors.initials: M
authors.lastname: Kato
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Norihiro
authors.initials: N
authors.lastname: Tsukagoshi
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000201:enzymology*; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D058619:Maltose-Binding Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D058619:Maltose-Binding Proteins; D015061:1,4-alpha-Glucan Branching Enzyme