Expression of proinflammatory cytokines and receptors by human fallopian tubes in organ culture following challenge with Neisseria gonorrhoeae.
Infection of the Fallopian tubes (FT) by Neisseria gonorrhoeae can lead to acute salpingitis, an inflammatory condition, which is a major cause of infertility. Challenge of explants of human FT with gonococci induced mRNA expression and protein secretion for the proinflammatory cytokines interleukin (IL)-1alpha, IL-1beta, and tumor necrosis factor alpha (TNF-alpha) but not for granulocyte-macrophage colony-stimulating factor. In contrast, FT expression of IL-6 and of the cytokine receptors IL-6R, TNF receptor I (TNF-RI), and TNF-RII was constitutive and was not increased by gonococcal challenge. These studies suggest that several proinflammatory cytokines are likely to contribute to the cell and tissue damage observed in gonococcal salpingitis.
Maisey
Kevin
K
Laboratorio de Inmunología de la Reproducción, Facultad de Química y Biología, Universidad de Santiago de Chile (USACH) Instituto Chileno de Medicina Reproductiva, Santiago, Chile. krmaisey@mi-mail.cl
Nardocci
Gino
G
Imarai
Monica
M
Cardenas
Hugo
H
Rios
Miguel
M
Croxatto
Horacio B
HB
Heckels
John E
JE
Christodoulides
Myron
M
Velasquez
Luis A
LA
D016207:Cytokines / Q000378:metabolism*; D005187:Fallopian Tubes / Q000276:immunology* / Q000382:microbiology*; D005260:Female; D006801:Humans; D007249:Inflammation; D007378:Interleukins / Q000378:metabolism; D018613:Microscopy, Confocal; D009344:Neisseria gonorrhoeae / Q000276:immunology* / Q000472:pathogenicity; D009924:Organ Culture Techniques; D018121:Receptors, Cytokine / Q000378:metabolism; D019947:Receptors, Interleukin-6 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D007378:Interleukins; D018121:Receptors, Cytokine; D019947:Receptors, Interleukin-6; D014409:Tumor Necrosis Factor-alpha