Choice of different dyes to label tyrosine and nitrotyrosine.
In this work, we will present some attempts to analyze tyrosine and nitrotyrosine using capillary electrophoresis and either UV-Visible detection or laser-induced fluorescence (LIF) detection. An argon ion (488 nm) laser is used for fluorescein isothiocyanate (FITC) and 7-fluoro-4-nitro-2,1,3-benzoxadiazole (NBD-F). A near infrared (780 nm) laser is used for NIR 780 derivatives. The UV-Visible limit of detection is 2.5 microM whereas it is in the range of 30 nM for LIF detection.
Massip
C
C
Laboratoire de Biochimie CHR Rangueil, 31043 Toulouse Cedex, France.
Riollet
P
P
Quemener
E
E
Bayle
C
C
Salvayre
R
R
Couderc
F
F
Caussé
E
E
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives* / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D019265:Spectroscopy, Near-Infrared; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry*
D005456:Fluorescent Dyes; C032930:7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole; C002744:3-nitrotyrosine; D014443:Tyrosine; D009327:4-Chloro-7-nitrobenzofurazan; D016650:Fluorescein-5-isothiocyanate