title: Double-stranded nef RNA interferes with human immunodeficiency virus type 1 replication.
abstract: RNA interference (RNAi) has been reported to be post-transcriptional gene silencing (PTGS) by approximately 500 nucleotide-(nt)-long double-stranded (ds) RNA that specifically targets homologous sequences of messenger RNA. In this report, we describe inhibition of HIV-1 transcription by synthetic dsRNAs constructed with mutated nef genes (nef dsRNAs) derived from long-term non-progressors (LTNPs) using cotransfection of the target gene-expressing plasmid and dsRNA. The effects of nef dsRNAs were examined with luciferase (Luc) reporter which is combined with the HIV-1 (SF2) LTR in persistently HIV-1-infected T cell and macrophage cell lines. At 48 hr, a defective nef dsRNA (556 nt) suppressed Luc activity more potently than did SF2 full-length nef dsRNA (744 nt), suggesting that approximately 500 nt-long nef dsRNA could interfere with the HIV-1 transcription.
authors.affiliation: Division of Nutritional Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Aichi 467-8603, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Shinya
authors.initials: S
authors.lastname: Omoto
authors.forename: Masaaki
authors.initials: M
authors.lastname: Mizuguchi
authors.forename: Hajime
authors.initials: H
authors.lastname: Mizukami
authors.forename: Harumi
authors.initials: H
authors.lastname: Okuyama
authors.forename: Noriko
authors.initials: N
authors.lastname: Okada
authors.forename: Nitin K
authors.initials: NK
authors.lastname: Saksena
authors.forename: Ebiamadon A
authors.initials: EA
authors.lastname: Brisibe
authors.forename: Kaori
authors.initials: K
authors.lastname: Otake
authors.forename: Yoichi R
authors.initials: YR
authors.lastname: Fuji
mesh_terms: D015693:Gene Products, nef / Q000235:genetics / Q000378:metabolism*; D020276:HIV Long-Term Survivors; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D034622:RNA Interference / Q000502:physiology*; D012330:RNA, Double-Stranded / Q000378:metabolism / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*; D054311:nef Gene Products, Human Immunodeficiency Virus
chemical_list: D015693:Gene Products, nef; D012330:RNA, Double-Stranded; D054311:nef Gene Products, Human Immunodeficiency Virus