title: Determination of trace isoflavone phytoestrogens in biological materials by capillary electrochromatography.
abstract: Capillary electrochromatography using a specialty monolithic matrix was utilized in developing a rapid and highly efficient separation of isoflavones in biological materials. Without a preconcentration technique, it is relatively easy to reach ppm-ppb concentrations of these compounds in soy-based foods and verify them structurally using a photodiode array detector. With on-column preconcentration, we were able to measure low-ppb levels in human serum. Using blood samples from human volunteers, whose diet was supplemented by a soy-based product, the method has been validated for high-throughput screening of isoflavones in clinical studies.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
authors.forename: Jason A
authors.initials: JA
authors.lastname: Starkey
authors.forename: Yehia
authors.initials: Y
authors.lastname: Mechref
authors.forename: Chang Kyu
authors.initials: CK
authors.lastname: Byun
authors.forename: Rosemary
authors.initials: R
authors.lastname: Steinmetz
authors.forename: John S
authors.initials: JS
authors.lastname: Fuqua
authors.forename: Ora H
authors.initials: OH
authors.lastname: Pescovitz
authors.forename: Milos V
authors.initials: MV
authors.lastname: Novotny
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods* / Q000592:standards; D004968:Estrogens, Non-Steroidal / Q000097:blood* / Q000302:isolation & purification; D005502:Food; D006801:Humans; D007223:Infant; D007225:Infant Food; D007231:Infant, Newborn; D007529:Isoflavones / Q000097:blood* / Q000302:isolation & purification; D008895:Milk, Human / Q000737:chemistry; D048789:Phytoestrogens; D028321:Plant Preparations; D013025:Glycine max / Q000737:chemistry
chemical_list: D004968:Estrogens, Non-Steroidal; D007529:Isoflavones; D048789:Phytoestrogens; D028321:Plant Preparations