Acute phase immune response to exercise coexists with decreased neutrophil antioxidant enzyme defences.
Long-duration or damaging exercise initiates reactions that resemble the acute phase response to infection and induces neutrophil priming for oxidative activity. Our objective was to establish the status of the antioxidant defences and of the oxidative equilibrium in the neutrophils of sportsmen prior to and after intense physical exercise. Nine voluntary male professional cyclists participated in this study. The exercise was a cycling mountain stage (171 km) and the cyclists took a mean +/- SEM of 270 +/- 12 min to complete it. We determined the activities of catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), the levels and activity of superoxide dismutase (SOD), the concentrations of ascorbate, glutathione and glutathione disulphide (GSSG) and DNA levels in neutrophils. The cycling stage decreased enzyme activities expressed per DNA units: CAT (33%), SOD (38%), GPx (65%); increased ascorbate concentration in neutrophils and decreased the GSH/GSSG ratio and the enzyme activities expressed per DNA units. Neutrophils could contribute to plasma antioxidant defences against oxidative stress induced by exercise because they probably provide antioxidant enzymes and ascorbate.
Tauler
Pedro
P
Laboratori de Ciències de l'Activitat Física and Departament de Biologia Fonamental i Ciències de la Salut. Edifici Guillem Colom, Facultat de Ciències, Universitat de les Illes Balears, Crta. Valldemossa km 7.5, E-07071 Palma de Mallorca, Balears, Spain.
Aguiló
Antoni
A
Cases
Nuria
N
Sureda
Antoni
A
Gimenez
Fernando
F
Villa
Gerardo
G
Córdova
Alfredo
A
Biescas
Antoni Pons
AP
D000210:Acute-Phase Reaction / Q000276:immunology*; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000097:blood; D001642:Bicycling; D002374:Catalase / Q000097:blood; D004247:DNA / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D015444:Exercise; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000737:chemistry / Q000201:enzymology*; D010084:Oxidation-Reduction; D013482:Superoxide Dismutase / Q000097:blood
D000975:Antioxidants; D004247:DNA; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; D001205:Ascorbic Acid