[Primary studies on the establishment of cytomegalovirus murine model].
Intraperitoneal inoculation of Murine cytomegalovirus(MCMV) into Balb/C mice at age of 4 weeks resulted in acute MCMV infection of the mice. The infected mice had body weight reduction, growth retardation, salivary gland swelling and death. A lethal dose was 5 x 10(6) PFU/ml of MCMV. Virus could be recovered from salivary gland and the infectivity titer of MCMV reached to a high titer of 2 x 10(5) PFU/ml. Plaque formation of MCMV in 3T3/Swiss albino cells was identified. Histopathological examination showed the appearance of MCMV-specific intracytoplasmic inclusion bodies in neuron cells of the mouse brain.
Ji
Y
Y
Department of Clinical Laboratory, Rui Jin Hospital, Shanghai 200025.
Zhu
Y
Y
Chen
S
S
D016475:3T3 Cells; D000818:Animals; D003586:Cytomegalovirus Infections; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C