title: Cloned cytosine deaminase gene expression of Bifidobacterium longum and application to enzyme/pro-drug therapy of hypoxic solid tumors.
abstract: Bifidobacterium longum is a nonpathogenic anaerobic bacterium among normal bacterial flora. Recently, it was reported that B. longum accumulated in hypoxic solid tumors. The gene of interest was expressed in transfected B. longum by the shuttle vector pBLES100 in solid tumors. In this report, we constructed pBLES100-S-eCD, which included the cytosine deaminase gene. We confirmed by western blotting that transfected B. longum produced cytosine deaminase. In addition, transfected B. longum produced cytosine deaminase that converted 5-fluorocytosine into 5-fluorouracil. B. longum could be useful for enzyme/pro-drug therapy of hypoxic solid tumors.
authors.affiliation: Department of Surgery, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Nakamura
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sasaki
authors.forename: Minoru
authors.initials: M
authors.lastname: Fujimori
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Yazawa
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Kano
authors.forename: Jun
authors.initials: J
authors.lastname: Amano
authors.forename: Shun'ichiro
authors.initials: S
authors.lastname: Taniguchi
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000493:pharmacokinetics* / Q000627:therapeutic use; D001644:Bifidobacterium / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D003001:Cloning, Molecular; D043525:Cytosine Deaminase; D005437:Flucytosine / Q000493:pharmacokinetics* / Q000627:therapeutic use; D005472:Fluorouracil / Q000493:pharmacokinetics* / Q000627:therapeutic use; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D000860:Hypoxia / Q000503:physiopathology; D009369:Neoplasms / Q000188:drug therapy; D009700:Nucleoside Deaminases / Q000235:genetics* / Q000378:metabolism*; D011355:Prodrugs / Q000493:pharmacokinetics* / Q000627:therapeutic use; D014162:Transfection; D014169:Transformation, Bacterial
chemical_list: D000964:Antimetabolites, Antineoplastic; D011355:Prodrugs; D005437:Flucytosine; D009700:Nucleoside Deaminases; D043525:Cytosine Deaminase; D005472:Fluorouracil