[Molecular mechanism of antisense glycosyltransferase oligonucleotide inhibiting human gliomas cell line SWO-38].
OBJECTIVE
It was reported that Globo series glycoshingolipids have relationship to many human cancers, and alpha 1, 4-galactosyltransferase(alpha 1, 4Gal-T) oligonucleotide is the specific glycosyltransferase for the synthesis of Globo series glycoshingolipids. This study was designed to evaluate the effects of antisense alpha 1, 4Gal-T oligonucleotide on human gliomas cell line SWO-38.


METHODS
SWO-38 glioma cells were reacted with 10 mumol/L antisense alpha 1, 4Gal-T oligonucleotide for 72 hours by means of lipofectin transfection. The growth inhibition was detected by colony-forming unit assay. DNA fragmentation was examined by flow cytometric analysis(FCM) and agarose gel eletrophoresis. The expressions of fas, p53, bax, and bcl-2 protein were determined by flow cytometric analysis (FCM).


RESULTS
Antisense alpha 1, 4Gal-T oligonucleotide signifantly inhibited cell growth (P < 0.01), induced the apoptosis (P < 0.05), downregulated expression of bcl-2 protein (P < 0.01) and upregulated expressions of fas and bax protein (P < 0.01), but did not influence p53 expression in glioma cell line SWO-38.


CONCLUSIONS
Antisense alpha 1, 4Gal-T oligonucleotide can significantly inhibit proliferation and induce apoptosis in human gliomas cell line SWO-38, which maybe due to the interactions of fas, bax, and bcl-2.
Zheng
Gang
G
Department of Oncology, First Military Medical University, Guangzhou 510282, P. R. China. zheng980918@263.net
Zhang
Ji-ren
JR
Liu
Fa-quang
FQ
Wang
Xiong-wen
XW
Zhang
Xin-yu
XY
D002455:Cell Division / Q000235:genetics / Q000502:physiology; D053938:DNA Fragmentation; D005434:Flow Cytometry; D015971:Gene Expression Regulation, Enzymologic; D005910:Glioma / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D016695:Glycosyltransferases / Q000235:genetics*; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D019014:fas Receptor / Q000096:biosynthesis
D016376:Oligonucleotides, Antisense; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D019014:fas Receptor; D016695:Glycosyltransferases