Dexamethasone attenuates by colchicine induced Fos expression in the rat deep cerebellar and vestibular nuclei.
1. The intent of the present study was to find out whether dexamethasone pretreatment may affect the induction of Fos protein in cell nuclei of the cerebellar vestibular neuronal complex (CVNC) elicited by central administration of colchicine. Specifically, the rate of the dexamethasone-sensitive cell population was analyzed and compared at different levels of the CVNC using a light microscopic avidin-biotin peroxidase immunohistochemistry. 2. Male Wistar rats were pretreated with dexamethasone 3 days prior (2.5 mg/kg/day, s.c.) and 24 h after an intracerebroventricular delivery of colchicine (60 microg/10 microL). Animals were sacrificed 48 h after colchicine treatment by a transcardial perfusion with fixative. 3. Dexamethasone in itself had no effect on the activity of cells of the CVNC. However, in colchicine treated animals, which exhibited a large number of Fos-positive cells over the entire CVNC, the dexamethasone elicited a substantial reduction in the number of the Fos-immunoreactive cells over the CVNC. Distinct dexamethasone dependent reduction (50-90%) of Fos-immunoreactivity was observed in each of the deep cerebellar nuclei. On the other hand, less number of dexamethasone-sensitive cells were recognized in the vestibular structures. From these, maximal Fos-inhibition by dexamethasone was recognized in the medial vestibular nucleus, however, even in this case the number of suppressed cells did not exceed 50%. 4. The results provide for the first time evidence about the dexamethasone dependent reduction of Fos-immunoreactivity in the cells of the CVNC in response to stimulation elicited by colchicine. The data also indicate that the glucocorticoids might be involved in the regulation of some functions of the CVNC under stress conditions.
Pirnik
Zdeno
Z
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlárska str. 3, 833 06 Bratislava, Slovakia.
Kiss
Alexander
A
D000818:Animals; D002529:Cerebellar Nuclei / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D003078:Colchicine / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D014726:Vestibular Nuclei / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism
D016760:Proto-Oncogene Proteins c-fos; D003907:Dexamethasone; D003078:Colchicine