title: Rapid diagnosis of invasive pulmonary aspergillosis by quantitative polymerase chain reaction using bronchial lavage fluid.
abstract: Polymerase chain reaction (PCR) is a sensitive method for detection of Aspergillus DNA in bronchoalveolar lavage fluid, but it has not yet been able to distinguish infection from contamination. We have established a technique to quantify Aspergillus DNA using a real-time PCR method to resolve this problem, and we report herein a successful application of real-time PCR to diagnose invasive pulmonary aspergillosis by comparing the amount of Aspergillus DNA in bronchial lavage fluid from an affected area to that from an unaffected area. This novel tool will provide rapid, sensitive, and specific diagnosis of pulmonary aspergillosis.
authors.affiliation: Department of Hematology & Oncology, University of Tokyo, Tokyo, Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Kawazu
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kanda
authors.forename: Susumu
authors.initials: S
authors.lastname: Goyama
authors.forename: Masataka
authors.initials: M
authors.lastname: Takeshita
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Nannya
authors.forename: Miyuki
authors.initials: M
authors.lastname: Niino
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Komeno
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nakamoto
authors.forename: Mineo
authors.initials: M
authors.lastname: Kurokawa
authors.forename: Shiho
authors.initials: S
authors.lastname: Tsujino
authors.forename: Seishi
authors.initials: S
authors.lastname: Ogawa
authors.forename: Katsunori
authors.initials: K
authors.lastname: Aoki
authors.forename: Shigeru
authors.initials: S
authors.lastname: Chiba
authors.forename: Toru
authors.initials: T
authors.lastname: Motokura
authors.forename: Nobuya
authors.initials: N
authors.lastname: Ohishi
authors.forename: Hisamaru
authors.initials: H
authors.lastname: Hirai
mesh_terms: D000328:Adult; D001228:Aspergillosis / Q000175:diagnosis* / Q000209:etiology / Q000235:genetics / Q000473:pathology; D001232:Aspergillus fumigatus / Q000235:genetics / Q000302:isolation & purification*; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D003199:Computer Systems; D018549:Cryptogenic Organizing Pneumonia / Q000209:etiology; D004271:DNA, Fungal / Q000032:analysis*; D005260:Female; D006086:Graft vs Host Disease / Q000209:etiology; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects / Q000627:therapeutic use; D015470:Leukemia, Myeloid, Acute / Q000150:complications; D008172:Lung Diseases, Fungal / Q000175:diagnosis* / Q000209:etiology / Q000235:genetics / Q000473:pathology; D009894:Opportunistic Infections / Q000175:diagnosis / Q000209:etiology / Q000235:genetics / Q000473:pathology; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014184:Transplantation, Homologous
chemical_list: D004271:DNA, Fungal; D007166:Immunosuppressive Agents