[Experimental research on inhibitory effect of alcohol extracts from Loranthus yadoriki Sieb. on coxsackie B3 virus].
OBJECTIVE
To investigate the antiviral effect of two components (B and C) in the alcohol extracts from Loranthus yadoriki Sieb for development of new antiviral drugs for coxsackie B3 virus(CVB3).


METHODS
Using ribavirin as positive control, the plaque reduction assay was adopted for pharmadynamic detection.


RESULTS
In the HEp-2 cell system, with respect to direct virucidal activity and the inhibition on CVB3 infection and multiplication, ED50s of component B were 2.32 micrograms.ml-1, 0.24 microgram.ml-1 and 1.91 micrograms.ml-1, respectively and those of component C, 1.44 micrograms.ml-1, 2.06 micrograms.ml-1 and 3.70 micrograms.ml-1 respectively. For the inhibition on multiplication of CVB3, ED50 of ribavirin was 7.55 micrograms.ml-1.


CONCLUSIONS
Treatment indexes (TI) of the direct virucidal activity and inhibitory effects of component B on CVB3 infection and multiplication 22.6, 219 and 27.5 respectively; component C, 115, 165 and 21.6; inhibition of ribavirin on CVB3 is 38, which is comparable to those of components B and C. It is thus suggested that the anti-CVB3 action of components B and C in the alcohol extracts from Loranthus yadoriki Sieb deserves to be exploited.
Wang
Z J
ZJ
Virus Research Institute, Hubei Medical University, Wuhan 430071, Hubei, China.
Yang
Z Q
ZQ
Huang
T N
TN
Wen
L
L
Liu
Y W
YW
D000998:Antiviral Agents / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D029822:Enterovirus B, Human / Q000187:drug effects*; D028183:Loranthaceae / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal