title: Helicobacter pylori organisms induce expression of activation and apoptotic surface markers on human lymphocytes and AGS cells: a cytofluorimetric evaluation.
abstract: Human peripheral blood mononuclear cells (PBMCs) were treated with Helicobacter pylori (Hp) organisms alone or with Hp-stimulated AGS cells (a gastric adenocarcinoma cell line). Hp organisms were able per se to increase the percentage of CD8 +/- CD95 +/- cells, while number of CD25+ cells and HLA-DR molecule expression increased following pretreatment with Hp-stimulated AGS cells. A comparison was made with a test system in which PBMCs were stimulated with Escherichia coli (Ec) organisms and colo-cells (a colon carcinoma cell line). In this case, CD95+ cells and CD25+ cells increased when the combination Ec organisms/colo-cells was present in the culture. On the other hand, Hp bacteria in combination with colo-cells were not able to induce activation and/or apoptotic surface markers on PBMCs, while Ec-stimulated AGS cells increased the expression of CD95 on PBMC. Finally, the direct interaction of AGS cells with Hp was able to induce higher expression of CD95 on gastric epithelial cells than Hp-stimulated PBMCs. Taken together, these data support the interplay between bacteria and epithelial cells in the course of Hp-mediated gastropathy.
authors.affiliation: Department of Internal Medicine, Immunology and Infectious Diseases, University of Bari, Bari, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Lembo
authors.forename: Luigi
authors.initials: L
authors.lastname: Caradonna
authors.forename: Thea
authors.initials: T
authors.lastname: Magrone
authors.forename: Maria Letizia
authors.initials: ML
authors.lastname: Mastronardi
authors.forename: Domenico
authors.initials: D
authors.lastname: Caccavo
authors.forename: Emilio
authors.initials: E
authors.lastname: Jirillo
authors.forename: Luigi
authors.initials: L
authors.lastname: Amati
mesh_terms: D000230:Adenocarcinoma / Q000382:microbiology* / Q000473:pathology; D017209:Apoptosis; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis; D008214:Lymphocytes / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013274:Stomach Neoplasms / Q000382:microbiology* / Q000473:pathology; D014407:Tumor Cells, Cultured; D019014:fas Receptor / Q000032:analysis
chemical_list: D006684:HLA-DR Antigens; D015375:Receptors, Interleukin-2; D019014:fas Receptor; D017493:Leukocyte Common Antigens