Anti-inflammatory and analgesic properties of the stem bark extract of Mitragyna ciliata (Rubiaceae) Aubrév. & Pellegr.
Mitragyna ciliata is widely used in traditional medicine for the treatment of inflammation, hypertension, headache, rheumatism, gonorrhoea and broncho-pulmonary diseases. In the present study, the anti-inflammatory and analgesic properties of the stem bark extract of M. ciliata were investigated. The stem bark of this plant was extracted over Soxhlet with hexane followed by another extraction with methanol. The resulting methanol extract was used for the pharmacological test. Anti-inflammatory activity was evaluated on the basis of the inhibitory effect of the extract on 5-lipoxygenase, and carrageenin-induced hind paw oedema in the rat. The methanol extract, at a dose of 19.2 microg/ml, exhibited no inhibition on 5-lipoxygenase. However, this extract administered per os (50 mg/kg) produced about 70% inhibition of carrageenin-induced paw oedema 1 h after administration. This inhibition was maintained to about 50% 2 h after administration. The dose of 50 mg/kg of MeOH extract significantly decreased sensitivity to pain from 78.75 to 107.5 g These findings suggest that extracts of the bark of M. ciliata, possess potent anti-inflammatory and analgesic effects. Chemical analysis of the extract showed the presence of alkaloids and kaempferol derivative which may be responsible for the anti-inflammatory properties.
Dongmo
A B
AB
Faculty of Science, University of Douala, PO Box 24157, Douala, Cameroon. adongmo@hotmail.com
Kamanyi
A
A
Dzikouk
G
G
Nkeh
B Chungag-Anye
BC
Tan
P V
PV
Nguelefack
T
T
Nole
T
T
Bopelet
M
M
Wagner
H
H
D000700:Analgesics / Q000737:chemistry / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000627:therapeutic use*; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D005260:Female; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy; D007962:Leukocytes / Q000187:drug effects / Q000201:enzymology; D008297:Male; D032065:Mitragyna / Q000737:chemistry*; D010146:Pain / Q000188:drug therapy*; D008517:Phytotherapy; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D018547:Plant Stems / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D001094:Arachidonate 5-Lipoxygenase