[Preparation and identification of monoclonal antibodies against Penicillium marneffei].
OBJECTIVE
To prepare and identify monoclonal antibodies (mAbs) against Penicillium marneffei.


METHODS
Recombinant mannoprotein1 (MP1) of Penicillium marneffei was used to immunize BALB/c mice, and anti-MP1 mAbs were obtained by means of hybridoma. Screening and identification of the mAbs were subsequently performed with indirect enzyme-linked immunosorbent assay and Western blotting, respectively.


RESULTS
Four hybridomas producing antibodies against Penicillium marneffei were obtained, and the IgG isotypes of the 4 mAbs were identified as IgG1 with affinity constants (K) of 8.2x10(-9), 4.7x10(-9), 6.5x10(-9) and 2.7x10(-9), respectively. Western blotting demonstrated specific recognition of Aspergillus fumigatus MP1 by the obtained mAbs.


CONCLUSIONS
The 4 hybridomas producing anti-MP1 mAbs with high specificity and affinity can be of significant value in the diagnosis of Penicilliosis marneffei infections.
Qiu
Li-wen
LW
Central Laboratory, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Peng
Dong-xian
DX
Xu
Hua
H
Jia
Juan
J
Che
Xiao-yan
XY
Yuen
Kwok-yung
KY
D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010407:Penicillium / Q000276:immunology*
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal