Th1 and Th1-inducing cytokines in Salmonella infection.
Thl and Thl-inducing cytokines and T cell responses were investigated in human salmonellosis. Serum IFN-gamma, IL-12 and IL-18 levels were increased significantly in patients with salmonellosis. The increase in serum IL-15 and IL-18 levels was more significant and prolonged in patients with the systemic form of salmonellosis than in those with the gastroenteric form. The serum IFN-gamma level was correlated significantly with IL-12 and IL18 levels, and the IL-15 level was correlated significantly with IL-18. Upon stimulation with Salmonella in vitro, mononuclear cells from salmonellosis patients produced significantly higher amounts of IFN-gamma and IL-12 compared with those from healthy controls. Anti-IL-12 moAb or anti-IL18 MoAb significantly inhibited Salmonella-induced IFN-gamma production in vitro. gamma delta T cells expressed significantly higher levels of IFN-gamma mRNA in salmonellosis patients than in healthy controls. The results suggest that Th1-inducing cytokines appear to be involved in the in vivo response against Salmonella infection, promoting IFN-gamma production by alpha beta and gamma delta T cells which plays a protective role against Salmonella.
Mizuno
Y
Y
Department of Paediatrics, Graduate School of Medical Sciences, Kyushu University and Fukuoka Children's Hospital and Medical Center for Infectious Diseases, Fukuoka, Japan. yumi-mi@mvc.biglobe.ne.jp
Takada
H
H
Nomura
A
A
Jin
C-H
CH
Hattori
H
H
Ihara
K
K
Aoki
T
T
Eguchi
K
K
Hara
T
T
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002454:Cell Differentiation; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D007223:Infant; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics; D018664:Interleukin-12 / Q000097:blood; D019409:Interleukin-15 / Q000032:analysis; D020382:Interleukin-18 / Q000097:blood; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012480:Salmonella Infections / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
D016207:Cytokines; D019409:Interleukin-15; D020382:Interleukin-18; D012333:RNA, Messenger; D016692:Receptors, Antigen, T-Cell, gamma-delta; D018664:Interleukin-12; D007371:Interferon-gamma