Clinical significance of nm23 expression and chromosome 17 numerical aberrations in primary gastric cancer.
The metastasis suppressor gene nm23 located on chromosome 17 might be one of the targets in deletions of chromosome 17. In this study, we analyzed the expression of nm23 and chromosome 17 aberrations in gastric cancer and assessed their clinicopathological and prognostic significance. In 103 gastric cancer patients, we examined nm23 expression by immunohistochemistry and detected chromosome 17 aberrations by fluorescence in situ hybridization. There was a significant difference in the expression of nm23 among differentiated histologic types (well > moderately > poorly) (p < 0.01). Negative expression of nm23 correlated with depth of invasion (p < 0.01), lymph node metastasis (p < 0.05), lymphatic invasion (p < 0.05), venous invasion (p < 0.05), poor prognosis (p < 0.05), and chromosome 17 loss (p < 0.05). Chromosome 17 aberrations broadly correlated with clinicopathological variables and were associated with poor prognosis (p < 0.05). Univariate analyses identified nm23 (p < 0.05), chromosome 17 aberrations (p < 0.05), tumor size (p < 0.01), depth of invasion (p < 0.0001), lymph node metastasis (P < 0.001), hepatic metastasis (p < 0.01), peritoneal dissemination (p < 0.01), and lymphatic invasion (p < 0.01) as significant prognostic factors. Multivariate analysis showed that expression of nm23 and chromosome 17 aberrations were not independent prognostic indicators. Our results indicate that negative expression of nm23 and chromosome 17 numerical aberrations correlate with tumor progression and poor prognosis but are not independent prognostic indicators.
Terada
Ryusuke
R
First Department of Surgery, School of Medicine, Nagasaki University, 1-7-1 Sakamoto, Nagasaki City, Nagasaki 852-8102, Japan. TE0403@aol.com
Yasutake
Toru
T
Nakamura
Shirou
S
Hisamatsu
Takashi
T
Sawai
Terumitsu
T
Yamaguchi
Hiroyuki
H
Nakagoe
Tohru
T
Ayabe
Hiroyoshi
H
Tagawa
Yutaka
Y
D000328:Adult; D000368:Aged; D002869:Chromosome Aberrations; D002886:Chromosomes, Human, Pair 17; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D020559:Monomeric GTP-Binding Proteins / Q000032:analysis*; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase; D011379:Prognosis; D013274:Stomach Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000401:mortality; D015996:Survival Rate; D014157:Transcription Factors / Q000032:analysis*
D054778:NM23 Nucleoside Diphosphate Kinases; D014157:Transcription Factors; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins