title: Effect of protease EPg222 obtained from Penicillium chrysogenum isolated from dry-cured ham in pieces of pork loins.
abstract: The fungal protease EPg222 obtained from Penicillium chrysogenum Pg222 isolated from dry-cured ham, was assayed for proteolytic activity in a meat model system based on sterile pieces of pork loins for 32 days. Treated samples showed a significative reduction of total high ionic strength-soluble proteins during the incubation period, as compared with a control incubated without enzyme, both on the surface and in the depth. SDS-PAGE analysis of this protein fraction showed higher hydrolysis of the main myofibrillar proteins H-meromyosin, actin, and tropomyosin in treated samples. Non-protein and amino acidic nitrogen were detected in higher amounts in enzyme-added than in control pieces of loins, both on the surface and in the depth. Thus, addition of enzyme EPg222 to whole pieces of meat results in an increase of protein hydrolysis. The effect of this enzyme could be of great interest for stimulating proteolysis in whole dry-cured meat pieces.
authors.affiliation: Higiene de los Alimentos, Facultad de Veterinaria, Universidad de Extremadura, Avda. de la Universidad s/n, 10071 Cáceres, Spain.
authors.forename: María J
authors.initials: MJ
authors.lastname: Benito
authors.forename: Mar
authors.initials: M
authors.lastname: Rodríguez
authors.forename: María J
authors.initials: MJ
authors.lastname: Sosa
authors.forename: Alberto
authors.initials: A
authors.lastname: Martín
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Córdoba
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D006868:Hydrolysis; D008460:Meat / Q000032:analysis* / Q000382:microbiology*; D008970:Molecular Weight; D009994:Osmolar Concentration; D010408:Penicillium chrysogenum / Q000201:enzymology* / Q000302:isolation & purification; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D012995:Solubility; D013552:Swine
chemical_list: D011506:Proteins; C471171:EPg222 protease, Penicillium chrysogenum; D010450:Endopeptidases