title: Antigen detection assay for identification of Penicillium marneffei infection.
abstract: Two recently produced monoclonal antibodies were used to develop an antigen capture enzyme-linked immunosorbent assay (ELISA) for rapid diagnosis of Penicillium marneffei. The method was evaluated with 53 patients with culture-confirmed penicilliosis and 240 controls. The diagnostic sensitivity, specificity, and accuracy of the ELISA were 92.45, 97.5, and 96.59%, respectively.
authors.affiliation: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand. scscy@mahidol.ac.th
authors.forename: Sansanee C
authors.initials: SC
authors.lastname: Chaiyaroj
authors.forename: Runglawan
authors.initials: R
authors.lastname: Chawengkirttikul
authors.forename: Stitaya
authors.initials: S
authors.lastname: Sirisinha
authors.forename: Pramuan
authors.initials: P
authors.lastname: Watkins
authors.forename: Yuttana
authors.initials: Y
authors.lastname: Srinoulprasert
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000946:Antigens, Fungal / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009181:Mycoses / Q000382:microbiology*; D010407:Penicillium / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D011933:Reagent Kits, Diagnostic