In vivo biocompatibility and analytical performance of intravascular amperometric oxygen sensors prepared with improved nitric oxide-releasing silicone rubber coating.
The in vivo biocompatibility and analytical performance of amperometric oxygen-sensing catheters prepared with a new type of nitric oxide (NO)-releasing silicone rubber polymer (DACA/N2O2 SR) is reported. The NO-release silicone rubber coating contains diazeniumdiolated secondary amine sites covalently anchored to a dimethylsiloxane matrix. Narrow diameter (0.9 mm, o.d.) silicone rubber tubing coated with this polymer can be employed to construct functional oxygen-sensing catheters that release NO continuously at levels > 1 x 10(-10) mol/cm2-min for more than 20 h. In vivo evaluation of such sensors within the carotid and femoral arteries of swine over a 16-h time period demonstrates that sensors prepared with the new NO-release coating exhibit no significant platelet adhesion or thrombus formation, but control sensors (non-NO release) implanted within the same animals do show a high propensity for cell adhesion and bulk clot formation. Furthermore, the in vivo analytical data provided by sensors fabricated with NO-release coatings (N = 9) are shown to be statistically equivalent to PO2 levels measured in vitro on discrete samples of blood. Control sensors (N = 9) placed within the same animals yield average PO2 values that are statistically different (p < or = 0.05) (lower) from both the levels measured on discrete samples and those provided by the NO-release sensors over a 16-h in vivo monitoring period.
Frost
Megan C
MC
Department of Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USA.
Rudich
Steven M
SM
Zhang
Huiping
H
Maraschio
Martín A
MA
Meyerhoff
Mark E
ME
D000818:Animals; D001784:Blood Gas Analysis / Q000295:instrumentation* / Q000379:methods; D002339:Carotid Arteries; D002408:Catheters, Indwelling; D020099:Coated Materials, Biocompatible; D004563:Electrochemistry; D005263:Femoral Artery; D006801:Humans; D009569:Nitric Oxide / Q000493:pharmacokinetics; D020030:Nitric Oxide Donors; D010100:Oxygen / Q000097:blood*; D012826:Silicone Elastomers; D013552:Swine
D020099:Coated Materials, Biocompatible; D020030:Nitric Oxide Donors; D012826:Silicone Elastomers; D009569:Nitric Oxide; D010100:Oxygen