title: Oxysterols induce apoptosis and accumulation of cell cycle at G(2)/M phase in the human monocytic THP-1 cell line.
abstract: The cytotoxic activity of oxysterols, 7 beta-hydroxycholesterol (7 beta-OHC) and 25-hydroxycholesterol (25-OHC), has been evaluated using various leukemia cell lines. Among the tested cell lines, both oxysterols showed the highest cytotoxicity to THP-1, human monocytic leukemia cell line. These oxysterols induced apoptosis through down-regulation of Bcl-2 expression and activation of caspases. Also, the oxysterols showed the accumulation at G(2)/M phase of cell cycle through down-regulation of cyclin B1 expression. Taken together, these results indicated that both 7 beta-OHC and 25-OHC inhibited the proliferation of THP-1 cells through apoptosis and cell cycle accumulation at G(2)/M phase.
authors.affiliation: Department of Biochemistry, College of Medicine Cheju National University, 1 Ara 1-dong, Jeju, Jeju-do, 690-756, South Korea.
authors.forename: Hee-Kyoung
authors.initials: HK
authors.lastname: Lim
authors.forename: Hee-Kyoung
authors.initials: HK
authors.lastname: Kang
authors.forename: Eun-Sook
authors.initials: ES
authors.lastname: Yoo
authors.forename: Bum-Joon
authors.initials: BJ
authors.lastname: Kim
authors.forename: Youn-Woo
authors.initials: YW
authors.lastname: Kim
authors.forename: Moonjae
authors.initials: M
authors.lastname: Cho
authors.forename: Jae-Hwa
authors.initials: JH
authors.lastname: Lee
authors.forename: Yun-Sil
authors.initials: YS
authors.lastname: Lee
authors.forename: Myung-Hee
authors.initials: MH
authors.lastname: Chung
authors.forename: Jin-Won
authors.initials: JW
authors.lastname: Hyun
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053938:DNA Fragmentation; D005434:Flow Cytometry; D016195:G2 Phase / Q000187:drug effects*; D018922:HL-60 Cells / Q000473:pathology; D006801:Humans; D006888:Hydroxycholesterols / Q000494:pharmacology*; D007938:Leukemia / Q000473:pathology; D007948:Leukemia, Monocytic, Acute / Q000473:pathology*; D015459:Leukemia-Lymphoma, Adult T-Cell; D008938:Mitosis / Q000187:drug effects*; D014407:Tumor Cells, Cultured
chemical_list: D006888:Hydroxycholesterols; C011724:cholest-5-en-3 beta,7 alpha-diol; C007997:25-hydroxycholesterol