Changes in hepatic enzyme activities in transgenic mice carrying human prototype c-Ha-ras gene treated with diethylnitrosamine.
Activities of malate dehydrogenase (MDH) and aspartate aminotransferase in the malate-aspartate shuttle were significantly increased in the cytosolic fractions of livers with early neoplastic symptoms such as swelling and discoloration in transgenic mice carrying the prototype human c-Ha-ras gene, Tg-rasH2 mice, which were administered with diethylnitrosamine (DEN) as a carcinogenic chemical at a dose of 200 mg/kg body weight. Cytosolic MDH/LDH (lactate dehydrogenase) (ML) ratio increased significantly and was considered to be a useful marker to characterize the energy metabolism at early neoplastic stage in livers of the Tg-rasH2 mice.
Arai
Toshiro
T
Division of Veterinary Biochemistry, Nippon Veterinary and Animal Science University, Musashino, Tokyo, Japan.
Ogawa
Takashi
T
Nakamura
Masako
M
Hosoya
Masaru
M
Ohnishi
Yasuyuki
Y
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000032:analysis; D002273:Carcinogens / Q000494:pharmacology*; D004052:Diethylnitrosamine / Q000494:pharmacology*; D004195:Disease Models, Animal; D004734:Energy Metabolism / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D015689:Oncogene Protein p21(ras) / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000032:analysis / Q000235:genetics; D019076:Transgenes / Q000235:genetics
D014408:Biomarkers, Tumor; D002273:Carcinogens; D004052:Diethylnitrosamine; D012313:RNA; D008291:Malate Dehydrogenase; D001219:Aspartate Aminotransferases; D015689:Oncogene Protein p21(ras)