[Hepatitis B virus antigen-induced specific TCR V beta gene subfamily amplifications and their diversity].
The human immune system confronts various antigens, then the recognitive structures of T lymphocytes must be diverse. In this study, we analyzed the TCR V beta 1-20 gene repertoire by reverse transcription-polymerase chain reaction (RT-PCR) in peripheral blood lymphocytes (PBLs) from individuals vaccinated with recombinant hepatitis B vaccine and PBLs from healthy donors stimulated by supernatants of the HepG2 2215 cell line transfected by HBV-DNA. The specific amplifications of V beta 6, 14, V beta 6, 15 were observed in PBLs after vaccination and stimulation by HepG2 2215 cell line respectively. The results suggest that the V beta segments may be the specific recognitive units for these antigens and involved in restriction and cytotoxicity.
Huang
S
S
Department of Molecular Biology, Guangdong Pharmacy College, Guangzhou 510224.
Cao
M
M
Luo
L
L
D002478:Cells, Cultured; D019674:Genes, T-Cell Receptor beta / Q000235:genetics*; D014644:Genetic Variation; D006514:Hepatitis B Surface Antigens / Q000494:pharmacology*; D017325:Hepatitis B Vaccines / Q000276:immunology; D006513:Hepatitis B e Antigens / Q000494:pharmacology*; D006515:Hepatitis B virus / Q000276:immunology; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D014407:Tumor Cells, Cultured; D014611:Vaccination
D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D006513:Hepatitis B e Antigens