title: The promoter of the classical MHC class I locus in rainbow trout (Oncorhynchus mykiss).
abstract: In rainbow trout only a single classical major histocompatibility complex (MHC) class I locus has been identified. In previous studies it was shown that this locus, Onmy-UBA, is characterised by extensive polymorphism and ubiquitous expression. In the present study the sequence of the promoter region of Onmy-UBA was analysed. Within this region several fragments for transcription regulation could be detected, including interferon-sensitive enhancer elements. The promoter region is very similar to that of classical MHC class I in higher vertebrates. In agreement with the interferon sensitive elements identified in its promoter, Onmy-UBA expression in rainbow trout gonad cells (RTG-2 cell line) was up-regulated after infection with infectious haematopoietic necrosis virus (IHNV).
authors.affiliation: Inland Station/National Research Institute of Aquaculture, Fisheries Research Agency, Tamaki, Mie, 519-0423, Japan.
authors.forename: Johannes Martinus
authors.initials: JM
authors.lastname: Dijkstra
authors.forename: Yasutoshi
authors.initials: Y
authors.lastname: Yoshiura
authors.forename: Ikunari
authors.initials: I
authors.lastname: Kiryu
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Aoyagi
authors.forename: Bernd
authors.initials: B
authors.lastname: Köllner
authors.forename: Uwe
authors.initials: U
authors.lastname: Fischer
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Ototake
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D005786:Gene Expression Regulation; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000276:immunology; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D015395:Histocompatibility Antigens Class I