[An experimental study on the dermal substitute of spongy collagen membrane].
OBJECTIVE
To investigate the practicability of a spongy collagen membrane as a dermal substitute.


METHODS
Porcine skin collagen was harvested and mixed and precipitated with chondroitin-6-sulfate, and then lyophilized for 48 hours to form sheets of highly porous membrane. The collagen membrane was embedded subcutaneously in SD rat's skin. The histological compatibility, the vascularization degree and degradation status were determined periodically by means of tissue sampling.


RESULTS
The collagen membrane possessed some degree of strength and tenacity with a structure consisting of multidinous interconnecting pores. It was easy to manipulate. Experimental subcutaneous embedding in SD rats indicated that the membrane exhibited good tissue compatibility, strong tendency to vascularization, with no evidence of acute inflammatory reaction, and slow degradation rate.


CONCLUSIONS
Spongy collagen membrane might be an optimal dermal substitute for wound coverage.
Yang
Jun
J
Department of Burns, Changhai Hospital, The Second Military Medical University Shanghai 200025, P.R. China.
Xiao
Shichu
S
Xia
Zhaofan
Z
D000818:Animals; D003094:Collagen / Q000378:metabolism*; D062109:Dermatologic Surgical Procedures; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000098:blood supply; D016038:Skin Transplantation; D017768:Skin, Artificial; D013552:Swine; D023822:Tissue Engineering / Q000379:methods; D014183:Transplantation, Heterologous
D003094:Collagen