title: [Analysis of proteins with altered expression in human esophageal squamous cell carcinomas].
abstract: OBJECTIVE
  Esophageal squamous cell carcinoma is characterized by high incidence and high mortality. This study was designed to identify the proteins dysregulated in ECSS by.
  
  
  METHODS
  Microdissection of routinely unstained frozen sections was used to procure cancer cells from seven esophageal squamous cell carcinomas, and esophageal epithelial cells from normal tissues adjacent to the tumors. By two-dimensional electrophoresis, protein profiles of normal and cancerous tissues were obtained. Selected proteins with altered expression in tumors were identified through MALDI-TOF-MS.
  
  
  RESULTS
  Compared the protein profiles of tumors with those of normal epithelia, 11 proteins were found as dysregulated in tumors. Among them, HSP27 was observed up-expressed and three isoforms of ANNEXIN I were seen down-expressed in cancerous tissues.
  
  
  CONCLUSIONS
  Two-dimensional electrophoresis coupled with mass spectrometry can contribute to rapidly screen dysregulated proteins in human maligancies. Altered expressions of HSP27 and ANNEXIN I were the frequent events in Chinese esophageal squamous cell carcinomas.
authors.affiliation: National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, P. R. China.
authors.forename: Shu-hua
authors.initials: SH
authors.lastname: Xia
authors.forename: Hai
authors.initials: H
authors.lastname: Hu
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Hu
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Cai
authors.forename: Ya-ling
authors.initials: YL
authors.lastname: Han
authors.forename: Bao-sheng
authors.initials: BS
authors.lastname: Chen
authors.forename: Fang
authors.initials: F
authors.lastname: Wei
authors.forename: Wan-tao
authors.initials: WT
authors.lastname: Ying
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Qian
authors.forename: You-yu
authors.initials: YY
authors.lastname: Cai
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.forename: Ming-rong
authors.initials: MR
authors.lastname: Wang
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D004938:Esophageal Neoplasms / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D009363:Neoplasm Proteins / Q000032:analysis*
chemical_list: D009363:Neoplasm Proteins