title: MG63 osteoblastic cell adhesion to the hydrophobic surface precoated with recombinant osteopontin fragments.
abstract: The hydrophobicity of biomaterials has been recognized as a limitation to the adequate function of anchorage-dependent cells when hydrophobic biomaterials are used for tissue engineering. This is due to flawed solid-state signals from cell adhesion. In this study, a recombinant osteopontin (rOPN17-169) fragment containing the cell adhesion motifs was expressed in E. coli and was precoated on the hydrophobic surface prior to osteoblastic MG63 cell culture. Precoating the hydrophobic surface with rOPN17-169 improved osteoblastic cell adhesion, which was blocked by soluble RGDS. The adhesion of MG63 cells to rOPN17-169 pre-coated surface-activated mitogen-activated protein kinases (MAPK) such as extracellular signal-receptor kinase 1/2, p38, and c-Jun N-terminal kinase (JNK). In addition, p38 MAPK was activated in response to a soluble factor of transforming growth factor-beta in the cells adhered to the hydrophobic surface via rOPN17-169. This suggests that rOPN17-169 precoated on the hydrophobic surface can allow osteoblastic cells to generate adhesion signals sufficient for cell adhesion, MAPK activation, and the cytokine activation of osteoblastic cells.
authors.affiliation: Laboratory for the Study of Molecular Biointerfaces, Department of Oral Anatomy, College of Dentistry, BK21HLS, and Intellectual Biointerface Engineering Center, Seoul National University, 28-22, YeonKun-Dong, ChongRo-Ku, Seoul 110-749, South Korea.
authors.forename: Yun Jung
authors.initials: YJ
authors.lastname: Lee
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Park
authors.forename: Woo Kul
authors.initials: WK
authors.lastname: Lee
authors.forename: Jea Seung
authors.initials: JS
authors.lastname: Ko
authors.forename: Hyun Man
authors.initials: HM
authors.lastname: Kim
mesh_terms: D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D003001:Cloning, Molecular; D020099:Coated Materials, Biocompatible; D017931:DNA Primers; D004926:Escherichia coli; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000502:physiology; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D053495:Osteopontin; D010446:Peptide Fragments / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000494:pharmacology; D012795:Sialoglycoproteins / Q000235:genetics / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases
chemical_list: D020099:Coated Materials, Biocompatible; D017931:DNA Primers; D010446:Peptide Fragments; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D053495:Osteopontin; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases