Long-term immunologic induction of donor-specific tolerance to skin allografts by bone marrow transplant in rabbits.
The induction of donor-specific tolerance to skin allografts was investigated in rabbits using bone marrow transplantation techniques reported to be effective in mice. Various routes of bone marrow transplantation (i.e., intravenous, portal venous, or intraosseous) were also examined. In regimen A, the animals were treated with portal venous injection of bone marrow cells from the donor on day 0 and intravenous injection of bone marrow cells from the same donor on posttransplant day 5. In regimen B, the animals were treated with portal venous and intraosseous injections of donor bone marrow cells on day 0 and intravenous injection of bone marrow cells from the same donor on posttransplant day 5. In regimen C, the animals were given intraosseous injection of donor bone marrow cells on day 0 and intravenous injection of bone marrow cells from the same donor on posttransplant day 5. It was found that regimens B and C were more effective than regimen A in prolonging allograft survival. The results demonstrate that induction of allograft tolerance can be achieved by bone marrow transplantation in a rabbit model. This protocol deserves further study in other large animal models.
Wang
Yi Biao
YB
Department of Plastic and Reconstructive Surgery, Kansai Medical University, Moriguchi City, Osaka, Japan. wangy@takii.kmu.ac.jp
Ogawa
Yutaka
Y
Doi
Hideaki
H
Kusumoto
Kenji
K
Jin
Tie Nan
TN
Ikehara
Susumu
S
D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology; D016026:Bone Marrow Transplantation; D006084:Graft Rejection / Q000276:immunology; D006085:Graft Survival; D007108:Immune Tolerance; D007959:Lymphocyte Culture Test, Mixed; D008212:Lymphocyte Depletion; D011817:Rabbits; D016038:Skin Transplantation / Q000276:immunology* / Q000473:pathology; D014184:Transplantation, Homologous / Q000276:immunology