title: Morphological changes of Pseudomonas pseudoalcaligenes in response to temperature selection.
abstract: Adaptation to novel environments usually entails morphological changes. The cell morphology of six experimental populations of Pseudomonas pseudoalcaligenes and their common ancestor were examined with scanning electron microscopy (SEM). The six experimental populations were propagated under different temperatures for 10 months: three of them cultured at constant normal temperature (35 degrees C) forming the control group, and the other three cultured at incremental higher temperatures (from 41 degrees to 47 degrees C) as the HT group. SEM showed the deformed and elongated cells in the 6-h cultures of both ancestral and control populations at 45 degrees C, indicating that 45 degrees C is stressful for the ancestral and the control populations. In contrast, the HT populations retained normal cell shape in the 6-h cultures at both 35 degrees C and 45 degrees C. The mean cell volumes of control and HT populations increased 29% and 34%, respectively, relative to the ancestor at their respective thermal regimens, suggestion that the culturing conditions might favor larger cells.
authors.affiliation: Department of Ecology & Biodiversity, University of Hong Kong, Hong Kong, China. bshi@hkucc.hku.hk
authors.forename: Bihong
authors.initials: B
authors.lastname: Shi
authors.forename: Xuhua
authors.initials: X
authors.lastname: Xia
mesh_terms: D000222:Adaptation, Physiological; D005075:Biological Evolution; D003470:Culture Media; D006358:Hot Temperature; D008855:Microscopy, Electron, Scanning; D011549:Pseudomonas / Q000254:growth & development* / Q000502:physiology / Q000648:ultrastructure*; D013696:Temperature
chemical_list: D003470:Culture Media