Use of real-time PCR to detect and quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" DNA.
A real-time PCR assay using Taqman probes was developed to detect and quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" in feline blood samples. The assay was rapid and sensitive and was successfully used to monitor the in vivo kinetics of cats experimentally infected with each species.
Tasker
Séverine
S
Department of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol, BS40 5DU, United Kingdom. s.tasker@bristol.ac.uk
Helps
Chris R
CR
Day
Michael J
MJ
Gruffydd-Jones
Tim J
TJ
Harbour
Dave A
DA
D000818:Animals; D002371:Cat Diseases / Q000382:microbiology; D002415:Cats; D004269:DNA, Bacterial / Q000032:analysis; D004195:Disease Models, Animal; D007700:Kinetics; D009174:Mycoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000382:microbiology* / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial