title: Laser-induced fluorescence and UV detection of derivatized aldehydes in air samples using capillary electrophoresis.
abstract: In this work, two capillary zone electrophoresis methodologies using UV absorption detection (214 nm) and laser-induced fluorescence detection (He/Cd laser, 325 nm excitation, 520 nm emission) of selected aldehydes (formaldehyde, acetaldehyde, propionaldehyde and acrolein) derivatized with dansylhydrazine (DNSH, 5-dimethylaminonaphthalene-1-sulfohydrazide) were proposed and validated. The aldehydes react with DNSH to form negatively charged molecules in methanolic medium. In both methodologies, nine DNSH-derivatives, including isomers of acetaldehyde, propionaldehyde and acrolein and two impurities were baseline separated in 20 mmol l(-1) phosphate buffer at pH 7.02, in less than 9 min. The limits of detection for the UV and LIF methodologies ranged from 1.1-9.5 microg l(-1) and 0.29-5.3 microg l(-1), respectively. The applicability of both methodologies to contemplate real samples was confirmed in the analysis of aldehyde-DNSH derivatives in indoor and outdoor air samples.
authors.affiliation: Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, 05508-900 São Paulo, SP, Brazil.
authors.forename: Elisabete Alves
authors.initials: EA
authors.lastname: Pereira
authors.forename: Emanuel
authors.initials: E
authors.lastname: Carrilho
authors.forename: Marina F M
authors.initials: MF
authors.lastname: Tavare
mesh_terms: D000388:Air / Q000032:analysis*; D000447:Aldehydes / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007834:Lasers; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D000447:Aldehydes