title: Simultaneous measurement of plasma concentrations and 13C-enrichment of short-chain fatty acids, lactic acid and ketone bodies by gas chromatography coupled to mass spectrometry.
abstract: A new method has been developed for the simultaneous measurement, in a reduced plasma sample, of concentration and 13C-isotopic enrichment of acetic, propionic, butyric, lactic, acetoacetic and beta-hydroxybutyric acids by gas chromatography coupled to mass spectrometry. After plasma deproteinisation, a diethylic extraction and a N-tert.-butyldimethylsilyl-N-methyltrifluoroacetamide derivatisation were performed. Both diethyl extraction and derivatisation procedures were optimised using the central composite designs methodology. The optimised method provides good linearity, intra-day and within-day repeatability. Except for beta-hydroxybutyric (49 microM) and acetoacetic acid (5 microM), detection limits were ranging between 0.2 and 0.7 microM allowing uses of this method for colonic metabolism studies.
authors.affiliation: Unité de Nutrition et Endocrinologie, Ecole Nationale Vétérinaire, Route de Gachet, BP 40706, 44307 cedex 03, Nantes, France.
authors.forename: N M
authors.initials: NM
authors.lastname: Moreau
authors.forename: S M
authors.initials: SM
authors.lastname: Goupry
authors.forename: J P
authors.initials: JP
authors.lastname: Antignac
authors.forename: F J
authors.initials: FJ
authors.lastname: Monteau
authors.forename: B J
authors.initials: BJ
authors.lastname: Le Bizec
authors.forename: M M
authors.initials: MM
authors.lastname: Champ
authors.forename: L J
authors.initials: LJ
authors.lastname: Martin
authors.forename: H J
authors.initials: HJ
authors.lastname: Dumon
mesh_terms: D000818:Animals; D002138:Calibration; D005227:Fatty Acids / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007657:Ketone Bodies / Q000097:blood*; D019344:Lactic Acid / Q000097:blood*
chemical_list: D005227:Fatty Acids; D007657:Ketone Bodies; D019344:Lactic Acid