Cloning and characterization of a novel splice variant of the brain-specific protein densin-180.
Densin-180 is a brain-specific synaptic protein of the o-sialoglycoprotein family. It functions in specific adhesion between presynaptic and postsynaptic membranes at glutamatergic synapses. We have cloned a novel splice variant of densin-180 from a fetal brain cDNA library and termed it LRRC7. It is located on 1p31 and consists of 8 exons. It encodes a putative protein of 216 amino acids which contains a nuclear localization signal and four leucine-rich repeats (LRRs). LRRs are 20-29 residue sequence motifs present in a number of proteins with diverse functions. Expression analysis of LRRC7 shows it is expressed ubiquitously while its splice variant densin-180 is brain-specific.
Wang
Liu
L
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, P.R. China.
Xu
Jian
J
Wu
Qihan
Q
Dai
Jianfeng
J
Ye
Xin
X
Zeng
Li
L
Ji
Chaoneng
C
Gu
Shaohua
S
Zhao
Robert Chunhua
RC
Xie
Yi
Y
Mao
Yumin
Y
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D012795:Sialoglycoproteins / Q000235:genetics*
C504461:LRRC7 protein, human; D012795:Sialoglycoproteins