title: Partial characterization of a 36-kDa protein from Clostridium botulinum type E that inhibits trypsin and chymotrypsin.
abstract: A 36-kDa trypsin inhibitor was purified from Clostridium botulinum type E culture supernatant by multiple molecular sieve and ion exchange chromatographic steps. The sequence of the amino-terminal 13 amino acid residues of this single-chain protein is Asn.Gln.Glu.Val.Phe.Asn.Met.Pro.Lys.Phe.Ser.Thr.Ala-. This novel protein that also inhibits chymotrypsin is produced by an organism that does not appear to produce any protease.
authors.affiliation: Department of Food Microbiology and Toxicology, University of Wisconsin, 1925 Willow Drive, Madison, WI 53706, USA.
authors.forename: Sakthivel
authors.initials: S
authors.lastname: Prabakaran
authors.forename: Bibhuti R.
authors.initials: BR
authors.lastname: DasGupta