title: The importance of alfalfa mosaic virus coat protein dimers in the initiation of replication.
abstract: Deletion and substitution mutations affecting the oligomerization of alfalfa mosaic virus (AMV) coat protein (CP) were studied in protoplasts to determine their effect on genome activation, an early step in AMV replication. The CP mutants that formed dimers, CPDeltaC9 and CPC-A(R)F, were highly active in initiating replication with 63-84% of wild-type (wt) CP activity. However, all mutants that did not form dimers, CPDeltaC18, CPDeltaC19, CPC-WFP, and CPC-W, were much less active with 19-33% of wt CP activity. The accumulation and solubility of mutant CPs expressed from a virus-based vector in Nicotiana benthamiana were similar to that of wt CP. Analysis of CP-RNA interactions indicated that CP dimers and CP monomers interacted very differently with AMV RNA 3' ends. These results suggest that CP dimers are more efficient for replication than CP monomers because of differences in RNA binding rather than differences in expression and accumulation of the mutant CPs in infected cells.
authors.affiliation: Department of Botany and Plant Pathology, Purdue University,West Lafayette, Indiana 47907, USA.
authors.forename: Jiwon
authors.initials: J
authors.lastname: Choi
authors.forename: Bong-Suk
authors.initials: BS
authors.lastname: Kim
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Zhao
authors.forename: Sue
authors.initials: S
authors.lastname: Loesch-Fries
mesh_terms: D017794:Alfalfa mosaic virus / Q000235:genetics / Q000502:physiology*; D002213:Capsid / Q000737:chemistry* / Q000502:physiology; D036022:Capsid Proteins; D016679:Genome, Viral; D012367:RNA, Viral / Q000502:physiology; D014026:Nicotiana / Q000821:virology; D014779:Virus Replication
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; C095691:coat protein, Alfalfa mosaic virus