title: T-box binding site mediates the dorsal activation of myf-5 in Xenopus gastrula embryos.
abstract: Myf-5, a member of the muscle regulatory factor family of transcription factors, plays an important role in the determination, development, and differentiation of the skeletal muscle. Factors that regulate the expression of myf-5 itself are not well understood. We show here that a T-box binding site in the Xenopus myf-5 promoter mediated the activation of myf-5 expression through specific interaction with nuclear proteins of gastrula embryos. The T-box binding site could be bound by and respond to T-box proteins. T-box genes could induce Xmyf-5. The results suggest that T-box proteins are involved in the specification of myogenic mesoderm and muscle development.
authors.affiliation: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, People's Republic of China.
authors.forename: Gu Fa
authors.initials: GF
authors.lastname: Lin
authors.forename: Xin
authors.initials: X
authors.lastname: Geng
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Qu
authors.forename: Fubin
authors.initials: F
authors.lastname: Wang
authors.forename: Ruiying
authors.initials: R
authors.lastname: Hu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ding
mesh_terms: D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002467:Cell Nucleus / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004268:DNA-Binding Proteins; D005775:Gastrula / Q000378:metabolism*; D017930:Genes, Reporter; D017403:In Situ Hybridization; D008648:Mesoderm / Q000166:cytology; D008957:Models, Genetic; D009124:Muscle Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D009132:Muscles / Q000166:cytology; D051787:Myogenic Regulatory Factor 5; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020825:T-Box Domain Proteins / Q000737:chemistry* / Q000378:metabolism; D015534:Trans-Activators; D014981:Xenopus; D029867:Xenopus Proteins
chemical_list: D004268:DNA-Binding Proteins; D009124:Muscle Proteins; C497419:Myf5 protein, Xenopus; D051787:Myogenic Regulatory Factor 5; D011500:Protein Synthesis Inhibitors; D020825:T-Box Domain Proteins; D015534:Trans-Activators; D029867:Xenopus Proteins; D003513:Cycloheximide; D004262:DNA Restriction Enzymes