title: Effect of multiple freeze-thaw cycles on detection of measles, mumps, and rubella virus antibodies.
abstract: We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4 degrees C until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on the ability to measure these antibodies by enzyme-linked immunoassays.
authors.affiliation: Mayo Vaccine Research Group, Mayo Clinic, Rochester, Minnesota 55905, USA.
authors.forename: Norman A
authors.initials: NA
authors.lastname: Pinsky
authors.forename: Jeanne M
authors.initials: JM
authors.lastname: Huddleston
authors.forename: Robert M
authors.initials: RM
authors.lastname: Jacobson
authors.forename: Peter C
authors.initials: PC
authors.lastname: Wollan
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Poland
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D015925:Cryopreservation; D004797:Enzyme-Linked Immunosorbent Assay; D005615:Freezing; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008457:Measles / Q000175:diagnosis / Q000276:immunology; D009107:Mumps / Q000175:diagnosis / Q000276:immunology; D012327:RNA Virus Infections / Q000175:diagnosis* / Q000276:immunology; D015203:Reproducibility of Results; D012409:Rubella / Q000175:diagnosis / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M