Post-transcriptional regulation of ura4+ gene expression by glucose in Schizosaccharomyces pombe.
Glucose-inducible gene expression is a fundamental cellular response for optimal cell growth, but identities of glucose-inducible genes and its regulatory mechanism remain largely elusive in Schizosaccharomyces pombe. Here we report that ura4+, encoding orotidine monophosphate decarboxylase (OMPdecase), shows glucose-inducible expression regulated at post-transcriptional level. The ura4+ mRNA level was rapidly decreased by approximately 50% within 20 min after glucose depletion and it was readily recovered upon glucose-readdition within 1 h. Glucose at above 2% similarly raised the transcript level of ura4+, while low concentration (0.1%) was not effective. Interestingly, control of mRNA turnover would be the main regulatory step of the glucose-dependent expression of ura4+. Moreover, stress-activated MAPK (SAPK) pathway was partially responsible for the glucose-regulated expression of ura4+ and rrg1+, another example of glucose-dependent mRNA stability control in S. pombe. These results suggest that the SAPK pathway might participate in the glucose-dependent regulation of ura4+ and rrg1+ mRNA stabilities.
Kim
Min Ji
MJ
School of Biological Sciences, Seoul National University, Seoul 151-742, Korea.
Kim
Minkyu
M
Park
Sang Dai
SD
D015152:Blotting, Northern; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000494:pharmacology*; D020928:Mitogen-Activated Protein Kinases / Q000187:drug effects; D012323:RNA Processing, Post-Transcriptional; D020871:RNA Stability; D012331:RNA, Fungal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism
D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; C448982:RRG1 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; C400810:ura4 protein, S pombe; D020928:Mitogen-Activated Protein Kinases; D005947:Glucose