title: Flagella and Motility in Actinobacillus pleuropneumoniae.
abstract: Actinobacillus pleuropneumoniae has been considered nonmotile and nonflagellate. In this work, it is demonstrated that A. pleuropneumoniae produces flagella composed of a 65-kDa protein with an N-terminal amino acid sequence that shows 100% identity with those of Escherichia coli, Salmonella, and Shigella flagellins. The DNA sequence obtained through PCR of the fliC gene in A. pleuropneumoniae showed considerable identity (93%) in its 5' and 3' ends with the DNA sequences of corresponding genes in E. coli, Salmonella enterica, and Shigella spp. The motility of A. pleuropneumoniae was observed in tryptic soy or brain heart infusion soft agar media, and it is influenced by temperature. Flagella and motility may be involved in the survival and pathogenesis of A. pleuropneumoniae in pigs.
authors.affiliation: Centro de Investigaciones en Ciencias Microbiológicas, Benemérita Universidad Autónoma de Puebla, Mexico. negretee@yahoo.com
authors.forename: Erasmo
authors.initials: E
authors.lastname: Negrete-Abascal
authors.forename: Magda E
authors.initials: ME
authors.lastname: Reyes
authors.forename: Rosa M
authors.initials: RM
authors.lastname: García
authors.forename: Sergio
authors.initials: S
authors.lastname: Vaca
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Girón
authors.forename: Octavio
authors.initials: O
authors.lastname: García
authors.forename: Edgar
authors.initials: E
authors.lastname: Zenteno
authors.forename: Mireya
authors.initials: M
authors.lastname: De La Garza
mesh_terms: D000189:Actinobacillus Infections / Q000662:veterinary; D016977:Actinobacillus pleuropneumoniae / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D003470:Culture Media; D005407:Flagella / Q000378:metabolism* / Q000648:ultrastructure*; D005408:Flagellin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009068:Movement; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology
chemical_list: D003470:Culture Media; D005408:Flagellin