title: Mercury and non-protein thiol compounds in the seagrass Posidonia oceanica.
abstract: Mercury concentrations, non-protein thiol levels and the enzyme activities of glutathione-S-transferase (GST) were measured in the blades and sheaths of the marine phanerogam Posidonia oceanica. The seagrass was collected in January and June and at three sites: the Bay of Rosignano (Italy) known for its mercury contamination, the north of the Lérins islands (Bay of Cannes, France), the Bay of Tonnara (Corsica, France). The two latter sites are considered as free of any known industrial inputs. Mercury concentrations and GST activities in both tissues were always higher in samples from Rosignano, particularly in June. Non-protein thiol levels were significantly higher in the blades than in the sheaths of P. oceanica from Tonnara and Lérins. In contrast, at Rosignano, the sheaths presented a significantly higher non-protein thiol concentration than the blades, particularly in June. Levels in the sheaths appeared to increase with the degree of pollution. Western Blot performed on sheaths of P. oceanica collected in June at Rosignano and Lérins revealed a characteristic band of GSTs at 31 kDa, proving the presence of the GST enzyme in this tissue. Mercury seemed to exert an influence upon non-protein thiol metabolism, including GST induction, in P. oceanica collected from the NW Mediterranean.
authors.affiliation: EqEl, University of Corsica, BP 52, 20250 Corte, France.
authors.forename: Lila
authors.initials: L
authors.lastname: Ferrat
authors.forename: Mauricette
authors.initials: M
authors.lastname: Gnassia-Barelli
authors.forename: Christine
authors.initials: C
authors.lastname: Pergent-Martini
authors.forename: Michèle
authors.initials: M
authors.lastname: Roméo
mesh_terms: D035961:Alismatales / Q000378:metabolism*; D015153:Blotting, Western; D004784:Environmental Monitoring / Q000379:methods*; D005978:Glutathione / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism*; D005982:Glutathione Transferase / Q000032:analysis / Q000378:metabolism; D019083:Mediterranean Region; D008628:Mercury / Q000032:analysis / Q000378:metabolism*; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D014874:Water Pollutants, Chemical; C402172:homoglutathione; D005982:Glutathione Transferase; D008628:Mercury; D005978:Glutathione