title: Lactic acid is absorbed from the small intestine of sheep.
abstract: A series of experiments was conducted in vivo on anaesthetized sheep to explore the hypothesis that lactic acid is absorbed from the small intestine of sheep. Test solutions varying in lactic acid concentration, pH, osmolarity, and with fixed physiological concentrations of volatile fatty acids (VFAs), K+, Na+, NH4 +, Cl-, and PO4 (-3), were separately introduced into clean, surgically sealed pouches. Studies were undertaken in 27 sheep, each with three pouches in the middle of the duodenum, jejunum, and ileum. Samples were taken at 15-minute intervals for 60 minutes to determine the absorption rates. The experimental results showed that L- and D-lactic acid were absorbed from the pouches of the duodenum, jejunum, and ileum throughout the 60 minutes. In the test solutions with pH 5.3, 420mOsmol/kg, and 12.5mM lactic acid that are in vivo conditions of light lactic acidosis, the mean absorption rates of D-lactic acid and L-lactic acid pooled from three pouches were similar, 0.07micro mol/cm2/min and 0.06micro mol/cm2/min, respectively, based on absorptive surface area. The mean absorption rates of DL-lactic acid from the duodenum, jejunum, and ileum pouches were almost the same, 0.14, 0.14, and 0.11micro mol/cm2/min, respectively. The absorption of lactic acid varied depending on lactic acid concentration, and there was a curvilinear relationship between lactic acid concentration and its absorption rate. A decrease in pH and osmotic pressure resulted in significant, corresponding increases in the absorption of lactic acid (P<0.0001 and P<0.05, respectively).
authors.affiliation: Science Center, Shantou University, Guangdong 515063, P.R. of China.
authors.forename: Zhaokun
authors.initials: Z
authors.lastname: Ding
authors.forename: Youqing
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism*; D019344:Lactic Acid / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics; D009997:Osmotic Pressure; D034561:Sheep, Domestic / Q000378:metabolism*
chemical_list: D019344:Lactic Acid