Association of Helicobacter pylori antioxidant activities with host colonization proficiency.
To assess the importance of two separate antioxidant activities in Helicobacter pylori, we tested the abilities of strains with mutations in either tpx (encoding thiolperoxidase) or ahpC (encoding alkyl hydroperoxide reductase [AhpC]) to colonize the stomachs of mice. The tpx strain was clearly more sensitive than the parent strain to both oxygen and cumene hydroperoxide. The strain colonized only 5% of the inoculated mice. Two different classes of oxygen-sensitive ahpC mutants in the type strain (ATCC 43504) were recently described (A. A. Olczak, J. W. Olson, and R. J. Maier, J. Bacteriol. 184:3186-3193, 2002). The same two classes of mutants were recovered upon ahpC mutagenesis of the mouse-adapted strain, SS1. Neither of these mutants was able to colonize mouse stomachs, whereas 78% of the mice inoculated with the parent strain became H. pylori positive.
Olczak
Adriana A
AA
Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA.
Seyler
Richard W
RW
Olson
Jonathan W
JW
Maier
Robert J
RJ
D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics / Q000254:growth & development*; D051379:Mice; D008810:Mice, Inbred C57BL; D008826:Microbial Sensitivity Tests; D009154:Mutation; D018384:Oxidative Stress; D010100:Oxygen / Q000494:pharmacology; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism*; D054464:Peroxiredoxins; D010940:Plant Proteins; D013270:Stomach / Q000382:microbiology*
D001555:Benzene Derivatives; D010940:Plant Proteins; D010544:Peroxidases; D054464:Peroxiredoxins; C087772:TPX1 protein, Lycopersicon esculentum; C007164:cumene hydroperoxide; D010100:Oxygen