Simultaneous measurement of plasma concentrations and 13C-enrichment of short-chain fatty acids, lactic acid and ketone bodies by gas chromatography coupled to mass spectrometry.
A new method has been developed for the simultaneous measurement, in a reduced plasma sample, of concentration and 13C-isotopic enrichment of acetic, propionic, butyric, lactic, acetoacetic and beta-hydroxybutyric acids by gas chromatography coupled to mass spectrometry. After plasma deproteinisation, a diethylic extraction and a N-tert.-butyldimethylsilyl-N-methyltrifluoroacetamide derivatisation were performed. Both diethyl extraction and derivatisation procedures were optimised using the central composite designs methodology. The optimised method provides good linearity, intra-day and within-day repeatability. Except for beta-hydroxybutyric (49 microM) and acetoacetic acid (5 microM), detection limits were ranging between 0.2 and 0.7 microM allowing uses of this method for colonic metabolism studies.
Moreau
N M
NM
Unité de Nutrition et Endocrinologie, Ecole Nationale Vétérinaire, Route de Gachet, BP 40706, 44307 cedex 03, Nantes, France.
Goupry
S M
SM
Antignac
J P
JP
Monteau
F J
FJ
Le Bizec
B J
BJ
Champ
M M
MM
Martin
L J
LJ
Dumon
H J
HJ
D000818:Animals; D002138:Calibration; D005227:Fatty Acids / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007657:Ketone Bodies / Q000097:blood*; D019344:Lactic Acid / Q000097:blood*
D005227:Fatty Acids; D007657:Ketone Bodies; D019344:Lactic Acid