title: Sterol effects and sites of sterol accumulation in Caenorhabditis elegans: developmental requirement for 4alpha-methyl sterols.
abstract: Caenorhabditis elegans requires sterol, usually supplied as cholesterol, but this is enzymatically modified, and different sterols can substitute. Sterol deprivation decreased brood size and adult growth in the first generation, and completely, reversibly, arrested growth as larvae in the second. After one generation of sterol deprivation, 10 ng/ml cholesterol allowed delayed laying of a few eggs, but full growth required 300 ng/ml. C. elegans synthesizes two unusual 4alpha-methyl sterols (4MSs), but each 4MS supported only limited growth as the sole sterol. However, addition of only 10 ng of cholesterol to 1,000 ng of 4MS restored full growth and egg-laying, suggesting that both a 4MS and an unmethylated sterol are required for development. Filipin stained sterols in only a few specific cells: the excretory gland cell, two amphid socket cells, two phasmid socket cells and, in males, spicule socket cells. Sterols were also present in the pharynx and in the intestine of feeding animals in a proximal-to-distal gradient. This non-random sterol distribution, the low concentration requirements, and the effects of 4MSs argues that sterols are unlikely to be used for bulk structural modification of cell membranes, but may be required as hormone precursors and/or developmental effectors.
authors.affiliation: Department of Physiology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Merris
authors.forename: William G
authors.initials: WG
authors.lastname: Wadsworth
authors.forename: Uttam
authors.initials: U
authors.lastname: Khamrai
authors.forename: Robert
authors.initials: R
authors.lastname: Bittman
authors.forename: David J
authors.initials: DJ
authors.lastname: Chitwood
authors.forename: John
authors.initials: J
authors.lastname: Lenard
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism* / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D002782:Cholestenes / Q000378:metabolism* / Q000494:pharmacology*; D002784:Cholesterol / Q000008:administration & dosage / Q000172:deficiency / Q000378:metabolism; D005453:Fluorescence; D015801:Helminth Proteins / Q000378:metabolism; D015394:Molecular Structure; D009747:Nutritional Physiological Phenomena; D011485:Protein Binding / Q000187:drug effects; D012098:Reproduction / Q000187:drug effects; D013261:Sterols / Q000378:metabolism* / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D002782:Cholestenes; D015801:Helminth Proteins; D013261:Sterols; C013605:4-methylcholest-8-en-3-ol; D002784:Cholesterol