Rational design of cytophilic and cytophobic polyelectrolyte multilayer thin films.
Nanostructured polyelectrolyte multilayer thin films electrostatically assembled alternately from such polymers as poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA) were investigated for their in vitro cell interactions. Not surprisingly, NR6WT cells, a highly adhesive murine fibroblast cell line, attached to many different multilayer combinations tested. However, PAH/PAA multilayers constructed at pH deposition conditions of 2.0/2.0 were completely bioinert. Analogous cell interactions were observed with PAH/poly(methacrylic acid) (PAH/PMA), PAH/sulfonated poly(styrene) (PAH/SPS), and poly(diallyldimethylammonium chloride)/SPS (PDAC/SPS) systems, thereby suggesting a general trend in the fibroblasts' response to multilayers. Specifically, highly ionically stitched films attracted cells, whereas weakly ionically cross-linked multilayers, which swell substantially in physiological conditions to present richly hydrated surfaces, resisted fibroblast attachment. Thus, by manipulating the multilayer pH or ionic strength assembly conditions or both, which in turn dictate the molecular architecture of the thin films, one may powerfully direct a single multilayer combination to be either cell adhesive or cell resistant.
Mendelsohn
Jonas D
JD
Department of Materials Science and Engineering and Center for Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Yang
Sung Yun
SY
Hiller
Jeri'Ann
J
Hochbaum
Allon I
AI
Rubner
Michael F
MF
D000180:Acrylic Resins; D015195:Drug Design; D004573:Electrolytes / Q000138:chemical synthesis* / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry*; D011109:Polymethacrylic Acids; D013053:Spectrophotometry; D013499:Surface Properties
D000180:Acrylic Resins; D004573:Electrolytes; D011108:Polymers; D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; C006903:carbopol 940