[Detection of TT virus DNA and sequence analysis of partial gene of TTV in sera from blood donors].
In order to investigate TTV infection in blood donors, 262 sera samples from blood donors were detected by nested--PCR method using primers from ORF1 of TTV genome DNA. The results showed that 18 of 58(31.0%) serum samples from blood donors with elevated transaminase level were positive for TTV DNA. 30 of 204 (14.7%) sera samples from blood donors with normal transaminase levels were positive for TTV DNA. There were significant difference between them (chi 2 = 38.1, P < 0.05). Five products of PCR for TTV DNA were purified and cloned to T Vector. The sequence analysis showed that there were above 97% nucleotide identity among AB008394 (from Japan), TTVCHNI (from China) and our 5 isolates from blood donors. The results suggest that there exists TTV infection among blood donors in China. TTV may be transmitted by blood transfusion. Our isolates, AB008394 and TTVCHN1 share a same genotype.
Huang
C
C
Shenzhen Baoan Blood Center, 518101.
Zhou
Y
Y
Chen
R
R
D001483:Base Sequence; D001782:Blood Donors; D004266:DNA Virus Infections / Q000821:virology*; D004279:DNA, Viral / Q000097:blood* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D022783:Torque teno virus / Q000235:genetics / Q000302:isolation & purification*
D004279:DNA, Viral