The endosomal epsilon-coatomer protein is involved in human adenovirus type 5 internalisation.
The effects of bafilomycin A1 and of the reduced level of endosomal epsilon-COP (coatomer protein) on the infectivity of human adenovirus type 5 were investigated in Coxsackie adenovirus receptor- (CAR-) transfected Chinese hamster ovary (CHO) cells. The endosomal proton pump inhibitor bafilomycin A1 was able to cause only partial inhibition. Using Id1F cells (an epsilon-COP thermosensitive mutant CHO cell line) the reduction of epsilon-COP level also had partial inhibitory effect. Based on these results and comparing them to existing models of the adenovirus entry, we propose a refined model in which there are two pathways of adenoviral entry: the first one involves the epsilon-COP as the downstream effector of the acidification and can be blocked by bafilomycin A1 and the second one is a pH-independent pathway.
Jeney
Cs
C
Institute of Medical Microbiology, Semmelweis University, H-1089 Budapest, Nagyvárad tér 4, Hungary. jencsa@xenia.sote.hu
Banizs
Boglárka
B
Dobay
Orsolya
O
Glatz
Katalin
K
Huszár
T
T
Adám
Eva
E
Nász
I
I
D000260:Adenoviruses, Human / Q000502:physiology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016466:CHO Cells; D002738:Chloroquine / Q000494:pharmacology; D020756:Coatomer Protein / Q000378:metabolism*; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D006224:Cricetinae; D004705:Endocytosis / Q000187:drug effects; D011992:Endosomes / Q000187:drug effects / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018942:Macrolides; D054328:Proton Pump Inhibitors; D017494:Proton Pumps / Q000378:metabolism; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
D000900:Anti-Bacterial Agents; C568771:CLMP protein, human; D020756:Coatomer Protein; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D018942:Macrolides; D054328:Proton Pump Inhibitors; D017494:Proton Pumps; D011991:Receptors, Virus; C057620:bafilomycin A; D002738:Chloroquine