[Preliminary study on apoptosis of BEL-7402 cells induced by Chinese herbs for warming yang and dispersing stasis].
OBJECTIVE
To investigate the antitumor mechanism of Chinese herbs for warming yang and dispersing stasis and observe the action of these herbs in inducing apoptosis of BEL-7402 cells.


METHODS
Apoptosis of BEL-7402 cells induced by the above-said herbs was detected through fast red tablet staining, flow cytometry and terminal uridine deoxynucleotidyl and labeling (TUNEL).


RESULTS
After treating BEL-7402 cells with the herbs typical apoptosis characteristics could be seen under light microscope and TUNEL positive cells were detectable. The apoptotic rate was 13.9% (drug concentration 0.5%) and 21.6% (drug concentration 1%)


CONCLUSIONS
Chinese herbs for warming Yang and dispersing stasis could induce apoptosis of BEL-7402 cells and this effect depends on drug concentration. Inducing the apoptosis of tumor cells may be part of the mechanism displayed by Chinese herbs for warming Yang and dispersing stasis in treating hepatoma.
Zhang
X
X
Beijing Military General Hospital, Beijing 100700, China.
Liu
D Q
DQ
Liu
Y Z
YZ
Wang
P
P
Chen
X Y
XY
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D010946:Plants, Medicinal; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal