[Identification of hepatitis C or/and G virus RNA in one tube by reverse transcription nested polymerase chain reaction].
Hepatitis C and G viruses belong to the flaviviridae family. They have similar modes of transmission. The dual infection of HGV and HCV were reported. A simultaneous detection for HCV or/and HGV was established by reverse transcription nested polymerase chain reaction(RT-nested PCR). The primers were derived from the 5' non-coding region of HCV and NS3 region of HGV, and the length of the PCR product was between 170 bp and 300 bp. RT-nested PCR can be finished in one test tube and HGV and/or HCV can be detected simultaneously in one RT-nest PCR. 153 samples were detected by his method. Our results showed that the established assay is useful for screening and identification of HCV or/and HGV RNA. This method is simple and specific practicable.
Tan
W
W
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052.
Xia
N
N
Cong
Y
Y
D020121:5' Untranslated Regions / Q000235:genetics; D005260:Female; D029407:GB virus C / Q000302:isolation & purification*; D016174:Hepacivirus / Q000302:isolation & purification*; D006526:Hepatitis C / Q000821:virology; D006801:Humans; D008297:Male; D020365:RNA Helicases; D012367:RNA, Viral / Q000097:blood*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases; D015163:Superinfection / Q000821:virology*; D017361:Viral Nonstructural Proteins / Q000235:genetics; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D020121:5' Untranslated Regions; D020365:RNA Helicases; D012367:RNA, Viral; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases