[A study of the expression of hypertrophic scar related cytoskeletal genes during early postburn stage].
OBJECTIVE
To screen the hypertrophic scar related cytoskeletal genes during early postburn stage, so as to explore their roles in postburn scar contraction.


METHODS
cDNA microarray chips containing 4096 human cDNAs were employed to investigate the cytoskeletal gene expression of the scar samples from human postburn hypertrophic scar. Furthermore, the expression of one of the cytoskeletal genes in hypertrophic scar tissue was studied by in situ hybridization.


RESULTS
Thirteen up - regulated cytoskeletal genes in 3 early postburn hypertrophic scar samples were identified. Moreover, the cells expressing human tropomyosin TM30 mRNA, one of the up - regulated cytoskeletal genes, were found increased in the early postburn hypertrophic scar samples.


CONCLUSIONS
In this study up - regulated expression of many hypertrophic scar related cytoskeletal genes was found in the scar samples during early postburn stage, and they might be important factors leading to postburn hypertrophic scar formation and contraction.
Ma
Bing
B
Institute of Burn Research, Southwestern Hospital, The Third Military Medical University, Chongqing 400038, P.R. China.
Wu
Jun
J
Yi
Shaoxuan
S
Wang
Zhenxiang
Z
He
Weifeng
W
Zhu
Jin
J
Luo
Gaoxing
G
Chen
Xiwei
X
D000293:Adolescent; D000328:Adult; D002056:Burns / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D017439:Cicatrix, Hypertrophic / Q000235:genetics*; D003599:Cytoskeleton / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014335:Tropomyosin / Q000235:genetics; D015854:Up-Regulation
D012333:RNA, Messenger; D014335:Tropomyosin