Multispectral imaging fluorescence microscopy for living cells.
Multispectral imaging technologies have been widely used in fields of astronomy and remote sensing. Interdisciplinary approaches developed in, for example, the National Aeronautics and Space Administration (NASA, USA), the Jet Propulsion Laboratory (JPL, USA), or the Communications Research Laboratory (CRL, Japan) have extended the application areas of these technologies from planetary systems to cellular systems. Here we overview multispectral imaging systems that have been devised for microscope applications. We introduce these systems with particular interest in live cell imaging. Finally we demonstrate examples of spectral imaging of living cells using commercially available systems with no need for user engineering.
Hiraoka
Yasushi
Y
Kansai Advanced Research Center, Communications Research Laboratory, Kobe, Japan. yasushi@crl.go.jp
Shimi
Takeshi
T
Haraguchi
Tokuko
T
D000818:Animals; D005057:Eukaryotic Cells / Q000166:cytology* / Q000378:metabolism; D036681:Fluorescence Recovery After Photobleaching; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence
D005456:Fluorescent Dyes