[Modulation of nerve growth factor on wound healing of burn].
OBJECTIVE
To investigate the effect of nerve growth factor(NGF) on the burn wound healing and to study the mechanism of burn wound healing.


METHODS
Six domestic pigs weighting around 20 kg were used as experimental animals. Twenty-four burn wound, each 2.5 cm in diameter, were induced on every pigs by scalding. Three different concentrations of NGF, 1 microgram/ml, 2.5 micrograms/ml, 5 micrograms/ml were topically applied after thermal injury, and saline solution used as control group. Biopsy specimens were taken at 3, 5 and 9 days following treatment and immunohistochemistry method was used to detect the epidermal growth factor(EGF), EGF receptor (EGF-R), NGF, NGF receptor (NGF-R), NGF, NGF-R, CD68 and CD3.


RESULTS
The expression of EGF, EGF-R, NGF, NGF-R CD68 and CD3 were observed in the experimental group, especially at 5 and 9 days, no expression of those six items in the control group.


CONCLUSIONS
NGF can not only act directly on burn wound, but also modulate other growth factors on the burn wound to accelerate the healing of burn wound.
Lü
G Z
GZ
Department of Burn Surgery, Wuxi No. 3 People's Hospital, Wuxi Jiangshu, P. R. China 214041.
Chen
Y L
YL
Yang
M J
MJ
D000818:Animals; D002056:Burns / Q000188:drug therapy* / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism; D020932:Nerve Growth Factor / Q000378:metabolism / Q000627:therapeutic use*; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism; D013556:Swine, Miniature; D014945:Wound Healing / Q000187:drug effects*
D020800:Receptor, Nerve Growth Factor; D004815:Epidermal Growth Factor; D020932:Nerve Growth Factor; D066246:ErbB Receptors