title: The role of focal adhesion kinase in the emigration of cells from confluent cultures.
abstract: We studied the effect of the modification of focal adhesion kinase (FAK) on the growth, migration and adhesion of C3H 10T1/2 cells. Cells transfected with plasmid coding for antisense FAK displayed a low level of FAK protein. Interestingly, the transfected cells achieved a higher saturation density at confluence, and displayed reduced adhesion and enhanced emigration from a confluent layer of cells when stimulated with fibronectin. In conclusion, it can be postulated that FAK plays an important role in the mechanism of contact inhibition.
authors.affiliation: Department of Cell Biology, Cancer Center, M. Skłodowska-Curie Institute of Oncology, W.K. Roentgen 5, 02-781Warsaw, Poland.
authors.forename: Halina
authors.initials: H
authors.lastname: Trembacz
authors.forename: Joanna
authors.initials: J
authors.lastname: Miłoszewska
authors.forename: Bozena
authors.initials: B
authors.lastname: Szaniawska
authors.forename: Maciej
authors.initials: M
authors.lastname: Małecki
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Przybyszewska
authors.forename: Tomasz
authors.initials: T
authors.lastname: Skorski
authors.forename: Przemysław
authors.initials: P
authors.lastname: Janik
mesh_terms: D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D016373:DNA, Antisense; D018076:DNA, Complementary / Q000378:metabolism*; D015536:Down-Regulation; D005353:Fibronectins / Q000378:metabolism; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; D051379:Mice; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*
chemical_list: D016373:DNA, Antisense; D018076:DNA, Complementary; D005353:Fibronectins; D011505:Protein-Tyrosine Kinases; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; C496051:Ptk2 protein, mouse