title: A role for chromatin remodeling in transcriptional termination by RNA polymerase II.
abstract: Chromatin remodeling can facilitate the recruitment of RNA polymerase II (Pol II) to targeted promoters, as well as enhancing the level of transcription. Here, we describe a further key role for chromatin remodeling in transcriptional termination. Using a genetic screen in S. pombe, we identified the CHD-Mi2 class chromatin remodeling ATPase, Hrp1, as a termination factor. In S. cerevisiae, we show that transcriptional termination and chromatin structure at the 3' ends of three genes all depend on the activity of the Hrp1 homolog, Chd1p, either alone or redundantly with the ISWI ATPases, Isw1p, and Isw2p. We suggest that chromatin remodeling of termination regions is a necessary prelude to efficient Pol II termination.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, OX1 3RE, Oxford, United Kingdom.
authors.forename: Claudia
authors.initials: C
authors.lastname: Alén
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Kent
authors.forename: Hannah S
authors.initials: HS
authors.lastname: Jones
authors.forename: Justin
authors.initials: J
authors.lastname: O'Sullivan
authors.forename: Agustín
authors.initials: A
authors.lastname: Aranda
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Proudfoot
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D001665:Binding Sites; D002843:Chromatin / Q000235:genetics / Q000648:ultrastructure*; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D008836:Micrococcal Nuclease / Q000378:metabolism; D012319:RNA Polymerase II / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D020413:3' Untranslated Regions; D002843:Chromatin; D004271:DNA, Fungal; D012319:RNA Polymerase II; D008836:Micrococcal Nuclease