title: One step purification of alpha(1)-acid glycoprotein from human plasma. Fractionation of its polymorphic allele products.
abstract: Alpha(1)-acid glycoprotein is a plasma protein that exhibits both microheterogeneity and polymorphism. Its purification from human plasma is usually performed using a sequence of different fractionation steps. Here we report a one-step isolation technique of this protein based upon pseudo-ligand affinity chromatography on immobilized Cibacron Blue F3GA at acidic pH. In addition, the use of two narrow pH elution buffers allows us to separate the two genetic products of this protein, which differ from each other by 21 amino acid substitutions. This technique will facilitate the study of the structural, biological and pharmacokinetic properties of each individual allele product.
authors.affiliation: Department of Microbiology and Immunology, Medical University of South Carolina, 29425, Charleston, SC, USA.
authors.forename: Federica
authors.initials: F
authors.lastname: Azzimonti
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Atchley
authors.forename: Catherine Anne
authors.initials: CA
authors.lastname: Morrison
authors.forename: Seetal
authors.initials: S
authors.lastname: Dodd
authors.forename: David W
authors.initials: DW
authors.lastname: Boulton
authors.forename: C Lindsay
authors.initials: CL
authors.lastname: DeVane
authors.forename: Philippe
authors.initials: P
authors.lastname: Arnaud
mesh_terms: D000483:Alleles; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D009961:Orosomucoid / Q000235:genetics / Q000302:isolation & purification*; D011110:Polymorphism, Genetic
chemical_list: D009961:Orosomucoid