Identification of an antigen-specific B cell population.
The difficulty in characterizing antigen-specific B cells that arise in the native B cell repertoire has been a formidable obstacle to understanding both protective and pathogenic antibody responses. We have developed a tetramer-based technique for identifying antigen-specific B cells. Biotin-labeled antigen is made tetrameric by interaction with streptavidin. The enhanced avidity of this antigenic compound for the B cell membrane permits the visualization, characterization and isolation of antigen-specific B cells.
Newman
Jeffrey
J
Room 405, Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Forch. Building, Bronx, NY 10461, USA.
Rice
Jeffrey S
JS
Wang
Chuansheng
C
Harris
Shannon L
SL
Diamond
Betty
B
D000818:Animals; D000941:Antigens / Q000737:chemistry; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D001710:Biotin; D051925:CD79 Antigens; D002462:Cell Membrane / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D007114:Immunization; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D020836:Protein Structure, Quaternary; D013154:Spleen / Q000166:cytology / Q000276:immunology; D019809:Streptavidin
D000941:Antigens; D015703:Antigens, CD; D051925:CD79 Antigens; C497884:Cd79b protein, mouse; D001710:Biotin; D019809:Streptavidin