Yellow fever virus replicons as an expression system for hepatitis C virus structural proteins.
Chimeric yellow fever virus (YF) RNAs were constructed in which the YF structural genes were replaced by the hepatitis C virus (HCV) structural genes or fusions between the YF and HCV structural genes. Interestingly, RNA replication required nucleotide complementarity between the 3'-located conserved sequence 1 and an RNA sequence located in the 5' end of the YF capsid sequence. The (chimeric-)HCV structural proteins were efficiently expressed and processed, and the native E1/E2 heterodimer was formed. However, no indication for the production of HCV-like particles was obtained.
Molenkamp
Richard
R
Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, The Netherlands.
Kooi
Engbert A
EA
Lucassen
Marjoleine A
MA
Greve
Sophie
S
Thijssen
Joyphi C P
JC
Spaan
Willy J M
WJ
Bredenbeek
Peter J
PJ
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005814:Genes, Viral; D016174:Hepacivirus / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D012093:Replicon; D015678:Viral Structural Proteins / Q000235:genetics*; D015005:Yellow fever virus / Q000235:genetics*
D012367:RNA, Viral; D015678:Viral Structural Proteins