[Effect of an vacuolar proton pump inhibitor bafilomycin A1 on the intracellular processing of markers for receptor-mediated and liquid phase endocytosis].
By means of subcellular fractionation in density Percoll gradients, immunoblotting and immunofluorescense, the effect of BafA1 on endocytosis of EGF-receptor complexes and horse radish peroxidase (HRP) in A431, HER14 and HC11 cell lines was studied. It was shown that the pretreatment of all used cell lines with BafA1 completely inhibited EGF degradation, but did not interfere with the delivery of significant portion of EGF-receptor complexes to late endosomes and lysosomes and transition of the receptor to juxtranuclear region. At the same time, BafA1 was found to dramatically inhibit the delivery of fluid phase marker HRP to late endosomes of A431 cells. The BafA1 effect on endocytosis of high concentrations of EGF was similar to that on HRP endocytosis. Regulatory mechanisms of early-to-late endosomal compartment transition are discussed.
Melikova
M S
MS
Institute of Cytology RAS, St. Petersburg.
Blagoveshchenskaia
A D
AD
Nikol'skiĭ
N N
NN
Kornilova
E S
ES
D016475:3T3 Cells; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004705:Endocytosis / Q000187:drug effects*; D011992:Endosomes / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D008247:Lysosomes / Q000378:metabolism; D018942:Macrolides; D051379:Mice; D054328:Proton Pump Inhibitors
D000900:Anti-Bacterial Agents; D018942:Macrolides; D054328:Proton Pump Inhibitors; D004815:Epidermal Growth Factor; C040929:bafilomycin A1; D066246:ErbB Receptors