[Study on the contents of HBV DNA in sera of patients with hepatic diseases by QPCR technique].
In order to test the content of serum HBV DNA for evaluating the effectiveness of clinical antiviral treatment and the relation of serum HBV DNA level to state of illness and prognosis, a QPCR method of energy transfer in amplification by semi-nested fluorescein labeled primer was established. Serum HBV DNA contents of 63 cases of hepatic diseases with abnormal liver function were detected by QPCR, PCR and ELISA. The positive detectable rate of the three methods were compared. The results showed that the positive detectable rate of QPCR was 82.54%, PCR 71.43% and ELISA 63.50%. The detectable rate of QPCR was compared with PCR, and uniformity rate of the both methods was 88.89%, P < 0.05; the positive results of QPCR were compared with ELISA, and their uniformity rate was 80.95%, P < 0.05; when serum average content (mean +/- s) of HBV DNA by QPCR method was compared with serum average titer of HBV by ELISA method, serum HBV-DNA average content of the group in which both QPCR and ELISA were positive was much higher than that of the group of QPCR(+) and ELISA(-), P < 0.05; serum QPCR-HBV-DNA of 30 cases of healthy persons were all negative. The results indicated that QPCR method is of highest positive detectable rate and more specific in detecting serum HBV DNA and had no nonspecific amplifying reaction. It has the superiority of detecting low level serum HBV DNA.
Zhao
H
H
Jinan Higher Medical Academy of PLA, Jinan 250022.
Jiang
P
P
Yin
H
H
D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008103:Liver Cirrhosis / Q000821:virology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods
D004279:DNA, Viral