Microarray analysis of neural stem cell differentiation in the striatum of the fetal rat.
1. Gene expression profiles in neural stem cell differentiation in vitro were determined by cDNA microarray analysis. 2. Total RNA was extracted and reverse transcripted into cDNA from differentiated and undifferentiated neural stem cells. The 33P labeled cDNA was hybridized with a cDNA microarray consisting of 14,000 human genes. 3. The results showed that a total of 1406 genes were differentially expressed, of which 148 genes exhibited more than twofold differences. Some genes were obviously activated while others were strongly repressed. These changes in gene expression suggest that differentiation is regulated by different genes at different expressional levels. By biological classification, the differentially expressed genes were divided into four functional categories: molecular function, biological process, cellular component, and new functional genes or ESTs. 4. These findings will be a valuable contribution for gene expression profiling and elucidation of neural stem cell differentiation mechanisms.
Wen
Tieqiao
T
Laboratory of Neural Molecular Biology, School of Life Sciences, Shanghai University, 99 Shangda Road, Shanghai 200436, People's Republic of China. tqwen2001@yahoo.com
Gu
Ping
P
Minning
Todd A
TA
Wu
Qi
Q
Liu
Min
M
Chen
Fuxue
F
Liu
Hao
H
Huang
Haihua
H
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D003342:Corpus Striatum / Q000166:cytology* / Q000502:physiology; D005260:Female; D005333:Fetus; D005786:Gene Expression Regulation / Q000502:physiology; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology