title: Detection of HLA polymorphisms by ligase detection reaction and a universal array format: a pilot study for low resolution genotyping.
abstract: We present our results in the identification of polymorphic sites within the second exon of the human leukocyte antigen A (HLA-A) region using the DNA microarray technology. Allele specific detection was performed by polymerase chain reaction followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. By this approach we confirmed 32 human samples previously characterized by direct DNA sequencing, thus demonstrating the interest of this approach.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biomediche and CISI, Università degli Studi di Milano, Segrate, Italy.
authors.forename: Clarissa
authors.initials: C
authors.lastname: Consolandi
authors.forename: Elena
authors.initials: E
authors.lastname: Busti
authors.forename: Cinzia
authors.initials: C
authors.lastname: Pera
authors.forename: Laura
authors.initials: L
authors.lastname: Delfino
authors.forename: Gian Battista
authors.initials: GB
authors.lastname: Ferrara
authors.forename: Roberta
authors.initials: R
authors.lastname: Bordoni
authors.forename: Bianca
authors.initials: B
authors.lastname: Castiglioni
authors.forename: Luigi Rossi
authors.initials: LR
authors.lastname: Bernardi
authors.forename: Cristina
authors.initials: C
authors.lastname: Battaglia
authors.forename: Gianluca
authors.initials: G
authors.lastname: De Bellis
mesh_terms: D011088:DNA Ligases / Q000378:metabolism; D005838:Genotype; D015234:HLA-A Antigens / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000706:statistics & numerical data; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D015234:HLA-A Antigens; D011088:DNA Ligases