Cloning and analysing the up-regulated expression of transthyretin-related gene (LR1) in rat liver regeneration following short interval successive partial hepatectomy.
OBJECTIVE
Cloning and analysing the up-regulated expression of transthyretin-related gene following short interval successive partial hepatectomy (SISPH) to elucidate the mechanism of differentiation, division, dedifferentiation and redifferentiation in rat liver regeneration (LR).


METHODS
Lobus external sinister and lobus centralis sinister, lobus centralis, lobus dexter, lobus candatus were removed one by one from rat liver at four different time points 4, 36, 36 and 36 hr (total time: 4 hr, 40 hr, 76 hr, 112 hr) respectively. Suppression subtractive hybridization (SSH) was carried out by using normal rat liver tissue as driver and the tissue following short interval successive partial hepatectomy (SISPH) as tester to construct a highly efficient forward-subtractive cDNA library. After screening, an interested EST fragment was selected by SSH and primers were designed according to the sequence of the EST to clone the full-length cDNA fragment using RACE (rapid amplification of cDNA end). Homologous detection was performed between the full-lenth cDNA and Genbank.


RESULTS
Forward suppression subtractive hybridization (FSSH) library between 0 h and 112 h following SISPH was constructed and an up-regulated full-length cDNA (named LR1), which was related with the transthyretin gene, was cloned by rapid amplification of cDNA end. It was suggested that the gene is involved in the cellular dedifferentiation in LR following SISPH.


CONCLUSIONS
Some genes were up-regulated in 112 h following SISPH in rat. LR(1) is one of these up-regulated expression genes which may play an important role in rat LR.
Xu
Cun-Shuan
CS
College of Life Science, Henan Normal University, Xinxiang 453002, Henan Province, China. xucs@x263.net
Li
Yu-Chang
YC
Lin
Jun-Tang
JT
Zhang
Hui-Yong
HY
Zhang
Yun-Han
YH
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015723:Gene Library; D006498:Hepatectomy; D008099:Liver / Q000502:physiology*; D008115:Liver Regeneration / Q000502:physiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011228:Prealbumin / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D015854:Up-Regulation / Q000502:physiology*
D011228:Prealbumin; D012333:RNA, Messenger; C474289:Ttr protein, rat