[Effects of culture conditions on callus growth and taxol formation of Taxus yunnanensis Cheng et L.K.Fu].
OBJECTIVE
To seek culture conditions suitable for the callus growth and taxol formation of Taxus yunnanensis.


METHODS
Determining the growth index and taxol content in the callus cultured under different illumination conditions or on different media.


CONCLUSIONS
Darkness, the combination of 0.1 mg.L-1BAP and 2,4-D 1.0 mg.L-1, and B5, DCR or 6,7-V basal medium were favorable for the callus growth and taxol formation of Taxus yunnanensis; high NO3- concentration in the medium promoted callus growth, and high NH4+ concentration greatly inhibited callus growth, but dramatically increased taxol content.
Chen
Y Q
YQ
Faculty of Life Sciences, Hubei University, Wuhan 430062, Hubei, China.
Zhu
W H
WH
Wu
Y Q
YQ
Hu
Q
Q
D003470:Culture Media; D046508:Culture Techniques; D017239:Paclitaxel / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry / Q000254:growth & development*; D020946:Taxus / Q000737:chemistry / Q000254:growth & development*
D003470:Culture Media; D017239:Paclitaxel