The effects of elutriates from PAH and heavy metal polluted sediments on Crassostrea gigas (Thunberg) embryogenesis, larval growth and bio-accumulation by the larvae of pollutants from sedimentary origin.
The release, bio-availability and toxicity of contaminants, when sediments are resuspended have been examined, studying concurrently their effects on the embryogenesis and on the larval growth of the Crassostrea gigas larvae and their bio-accumulation in those organisms. Three characteristic sediments have been selected (one contaminated by PAHs, a second by heavy metals and the last by the both pollutants). The organisms were directly exposed to elutriates obtained from each sediment or fed on algae (Isochrysis galbana) contaminated with the same elutriates. The elutriates used in this study show contamination levels similar to those observed in some polluted coastal and estuary environments. The larval growth test has appeared to be more sensitive that the embryotoxicity test. The biological effects and the contaminant bio-accumulation were more pronounced when larvae were directly exposed to different elutriates. In the case of PAHs, the contamination of algae was sufficient to lead to effect on the larval growth of the Crassostrea gigas. In all cases, a fraction of contaminants adsorbed on suspended particles was bio-available and accumulated by the larvae. This study has shown that resuspending polluted sediments constitutes a threat to pelagic organisms and than the C. gigas larval growth may be proposed as a test to protect the most sensitive areas.
Geffard
Olivier
O
Laboratoire de Physico-Toxicochimie des Systèmes Naturels, Université de Bordeaux 1, 33405 Talence Cedex, France. o.geffard@lpte.u-bordeaux.fr
Budzinski
Hélène
H
His
Edouard
E
D000818:Animals; D001267:Atlantic Ocean; D001681:Biological Assay; D001682:Biological Availability; D004625:Embryo, Nonmammalian; D056890:Eukaryota / Q000737:chemistry; D019015:Geologic Sediments / Q000737:chemistry*; D066298:In Vitro Techniques; D007814:Larva; D019216:Metals, Heavy / Q000009:adverse effects* / Q000302:isolation & purification; D010125:Ostreidae / Q000187:drug effects* / Q000196:embryology / Q000254:growth & development; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects* / Q000302:isolation & purification; D012623:Seawater; D018675:Toxicity Tests / Q000379:methods; D014873:Water Pollutants / Q000009:adverse effects*
D019216:Metals, Heavy; D011084:Polycyclic Aromatic Hydrocarbons; D014873:Water Pollutants