title: Detection of the anti-P53 antibodies in dogs with tumors.
abstract: To detect the anti-P53 antibodies of dogs with tumors, a GST-recombinant canine (rc) P53 fusion protein was expressed and purified. Immunoblot analysis was performed using this GST-rcP53 fusion protein as an antigen and serum samples from dogs suffering from tumors as primary antibodies. Out of 16 serum samples obtained from various tumor cases, four samples showed reaction with GST-rcP53. In contrast, serum from other 12 dogs with tumors, four dogs with non-neoplastic diseases and two control healthy dogs (as controls) did not show any reaction with GST-rcP53 in immunoblotting. The p53 gene mutation and the P53 protein expression were examined, using the tumor tissues to explore the relationship between the existence of the GST-rcP53 bands, gene mutations of p53 and the accumulation of P53 protein. One case, which showed a clear GST-rcP53 band, had a point mutation of the p53 cDNA and showed nuclear accumulation of P53 protein. These results suggest that the anti-P53 antibodies are also produced in tumor dogs with p53 gene mutations.
authors.affiliation: Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, Yoshida, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Kanaya
authors.forename: Masaru
authors.initials: M
authors.lastname: Okuda
authors.forename: Naomi
authors.initials: N
authors.lastname: Toyama
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Oikawa
authors.forename: Hisashi
authors.initials: H
authors.lastname: Inokuma
authors.forename: Masahiro
authors.initials: M
authors.lastname: Morimoto
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Hayashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Une
authors.forename: Munekazu
authors.initials: M
authors.lastname: Nakaichi
authors.forename: Yasuho
authors.initials: Y
authors.lastname: Taura
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
authors.forename: Takafumi
authors.initials: T
authors.lastname: Onishi
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology*; D004252:DNA Mutational Analysis; D018076:DNA, Complementary / Q000235:genetics; D004283:Dog Diseases / Q000235:genetics / Q000276:immunology*; D004285:Dogs; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D009369:Neoplasms / Q000276:immunology* / Q000662:veterinary*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000276:immunology*
chemical_list: D000906:Antibodies; D018076:DNA, Complementary; D016159:Tumor Suppressor Protein p53