Multianalyte pin-printed biosensor arrays based on protein-doped xerogels.
We report the first biosensor arrays based on pin printing protein-doped xerogels. The individual biosensor elements are on the order of 100 microm in diameter. Arrays are formed (1) onto a planar substrate that is excited by an external source (laser) or (2) directly on the face of a light-emitting diode. We illustrate the potential of our approach by fabricating, testing, and characterizing four types of pin-printed biosensor arrays (PPBSA) for the simultaneous detection of glucose and O2. The analytically reliable operating ranges for the PPBSAs are 0.1-10 mM for glucose and 0.1-100% for O2. The PPBSAs exhibit short- and long-term reproducibilities of no worse than 4 and 8%, respectively. The overall array-to-array response reproducibilities are < or = 12%. These results demonstrate for the first time the combination sol-gel processing and pin printing methods as a way to rapidly form ensembles of integrated, reusable, and stable biosensor arrays for simultaneous multianalyte detection.
Cho
Eun Jeong
EJ
Department of Chemistry, Natural Sciences Complex, University at Buffalo, The State University of New York, Buffalo, New York 14260-3000, USA.
Tao
Zunyu
Z
Tehan
Elizabeth C
EC
Bright
Frank V
FV
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D005782:Gels / Q000737:chemistry; D005947:Glucose / Q000032:analysis; D010100:Oxygen / Q000032:analysis; D011506:Proteins / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005782:Gels; D011506:Proteins; D005947:Glucose; D010100:Oxygen