[Mechanism of anti-inflammatory action of Huanglian Jiedu decoction a traditional Chinese prescription].
OBJECTIVE
To elucidate the mechanism of anti-inflammatory action of Huanglian Jiedu Decoction (HJD).


METHODS
An acute inflammatory air-pouch was established in mice by s.c. injecting air and 1% carrageenan. A mouse endotoxeamia induced by lipopolysaccharide was also used. Splenocyte proliferation was quantified with 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetery. NO2- concentration was examined by a Griess reagent and interleukin-1 activity was evaluated in thymocyte proliferation.


RESULTS
HJD significantly inhibited the leukocyte infiltration and PGE2 production in the mouse air-pouch model. In the in vitro assay, HJD significantly inhibited concanavalin A(Con A)-induced transformation of spleen cells isolated from the endotoxeamia mice, but did not influence that from the normal mice. HJD did not affact Con A-induced interleukin-2 production in splenocytes in either normal or endotoxeamia mice. HJD remarkably reduced interleukin-1 and NO production in peritoneal macrophages elicited by lipopol ysaccharide.


CONCLUSIONS
HJD displays an anti-inflammatory effect mainly through inhibiting the production of inflammatory cytokines including IL-1, NO and PGE2.
Wang
L J
LJ
Department of Pharmacology for Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, Jiangsu, China.
Xu
Q
Q
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D019446:Endotoxemia / Q000378:metabolism; D007375:Interleukin-1 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis; D010946:Plants, Medicinal
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007375:Interleukin-1; D009569:Nitric Oxide