Detection of HLA polymorphisms by ligase detection reaction and a universal array format: a pilot study for low resolution genotyping.
We present our results in the identification of polymorphic sites within the second exon of the human leukocyte antigen A (HLA-A) region using the DNA microarray technology. Allele specific detection was performed by polymerase chain reaction followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. By this approach we confirmed 32 human samples previously characterized by direct DNA sequencing, thus demonstrating the interest of this approach.
Consolandi
Clarissa
C
Dipartimento di Scienze e Tecnologie Biomediche and CISI, Università degli Studi di Milano, Segrate, Italy.
Busti
Elena
E
Pera
Cinzia
C
Delfino
Laura
L
Ferrara
Gian Battista
GB
Bordoni
Roberta
R
Castiglioni
Bianca
B
Bernardi
Luigi Rossi
LR
Battaglia
Cristina
C
De Bellis
Gianluca
G
D011088:DNA Ligases / Q000378:metabolism; D005838:Genotype; D015234:HLA-A Antigens / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000706:statistics & numerical data; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D015234:HLA-A Antigens; D011088:DNA Ligases