Treadmill exercise increases cell proliferation in dentate gyrus of rats with streptozotocin-induced diabetes.
Diabetes mellitus is a common and serious metabolic disorder in humans. Streptozotocin (STZ)-induced diabetic rats were shown to produce a significant reduction in the number of proliferating cells in the dentate gyrus. In the present study, the effect of treadmill exercise on cell proliferation in the dentate gyrus of rats with STZ-induced diabetes was investigated via immunohistochemistry. Male Sprague-Dawley rats were divided into four groups: the control-rest group, the control-exercise group, the diabetes-rest group, and the diabetes-exercise group. Each of the animals of the diabetes groups was given a single injection of STZ (50 mg/kg) intraperitoneally. All animals were injected with 5-bromo-2'-deoxyuridine (50 mg/kg) for six consecutive days, starting on the second day after STZ injection, and rats of the exercise groups were made to run on treadmill for 30 min each day over the same period. On the eighth day of the experiment, all animals were sacrificed. In the present results, it was shown that cell proliferation in the dentate gyrus is suppressed under diabetic conditions, and that treadmill is effective in enhancing hippocampal granular cell proliferation in rats with STZ-induced diabetes. These results raise the possibility that treadmill exercise is of help in the alleviation of the central neural sequelae of diabetes mellitus.
Kim
Hyun-Bae
HB
Department of Physiology, College of Medicine, Kyung Hee University, 1 Hoigi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea.
Jang
Mi-Hyeon
MH
Shin
Min-Chul
MC
Lim
Baek-Vin
BV
Kim
Young-Pyo
YP
Kim
Kyung-Ji
KJ
Kim
Ee-Hwa
EH
Kim
Chang-Ju
CJ
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D001973:Bromodeoxyuridine / Q000032:analysis; D002455:Cell Division; D018891:Dentate Gyrus / Q000473:pathology*; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D005082:Physical Exertion; D051381:Rats; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D001973:Bromodeoxyuridine