[Modified flow cytometry assay for CD62p expression of preserved platelets].
CD62p expression was an important monitoring parameter for preserved platelets quality. To setup an optimized flow cytometry assay for preserved platelets based on the CD62p expression on platelets, the platelet samples were collected, 0.1 mmol/L persantine and 1.1 mmol/L EDTA were added into the modified TB S used to replace PBS dilution; the methodological evaluation were carried out. Results showed that 0.1 mmol/L persantine and 1.1 mmol/L EDTA achieved to prevent platelets activation during the test procedure. The favorable negative or positive samples were prepared for check of fluorescence antibody's quality to ensure the validity of results. CD61 was used to identify platelets for assay and improve veracity of assay. The special injector was also replaced by special big syringe needle for blood collection to reduce in vitro artifacts, and the prepared sample can be steady-going for 48 hours at 4 degrees C after fixed by 1% paraformaldehyde. It is concluded that this flow cytometry assay for CD62p positive platelets is simple and efficient.
Ouyang
Xi-Lin
XL
Department of Blood Transfusion, General Hospital of PLA, Center of Clinical Transfusion of PLA, Beijing 100853, China. ouyang70@plagh.com.cn
Liu
Jing-Han
JH
Shi
Qun
Q
Gao
Da-Yong
DY
D001792:Blood Platelets / Q000737:chemistry*; D001793:Blood Preservation; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D019007:P-Selectin / Q000097:blood*
D019007:P-Selectin