[Morphological observation of mesenchymal stem cells cultured with allogenic decalcified bone matrix].
OBJECTIVE
To investigate the feasibility of using decalcified bone matrix loaded with mesenchymal stem cells (MSCs) derived from adult human bone marrow as scaffolds for bone tissue engineering.


METHODS
Allogenic decalcified bone matrix was prepared. MSCs were isolated from adult human bone marrow with monocyte-separating medium and then cultured in basic medium of mesenchymal stem cells (MSC). The third generation of the cultured cells were identified by FITC-antiCD105, seeded onto allogenic decalcified bone matrix followed by observation under scanning electron microscope and invert microscope a week later.


RESULTS
Rapid proliferation of both primary and subcultured MSCs was observed, and CD105-positive cells reached 64.1% among the third generation of the cells. MSCs also grew well and proliferated rapidly after being cultured with decalcified bone matrix.


CONCLUSIONS
Naive MSCs are ideal seeding cells for bone tissue engineering, and the allogenic decalcified bone matrix loaded with MSCs may be used as a good scaffold.
Liu
Xiao-jing
XJ
Department of Anatomy First Military Medical University, Guangzhou 510515, China.
Chen
Ying
Y
Yuan
Lin
L
Yu
Lei
L
Wang
Fei
F
Liao
Hua
H
D001857:Bone Matrix / Q000502:physiology*; D002455:Cell Division; D002478:Cells, Cultured; D006801:Humans; D008648:Mesoderm / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D013234:Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D023822:Tissue Engineering / Q000379:methods*