Quantitative assessment of antigen-specific CD8+ T cells in the mouse: application to vaccine research.
An effective HIV vaccine will likely need to induce potent and broad-based immunity, including CD8+ T cell responses. Hence, a quantitative assay to measure such responses in animal models will be important in the evaluation of candidate HIV vaccines. We show here that a single immunization with HIV DNA vaccines, followed by challenge with recombinant vaccinia virus expressing the relevant HIV antigen, allows quantitative assessment of CD8+ T cell responses. These responses can be profound (>30% of total CD8+ T cells) and directly reflect the level of memory CD8+ T cells at the time of challenge. Therefore, this assay will facilitate the selection of promising HIV vaccine candidates for further evaluation.
Otten
Gillis R
GR
Vaccines Research, Chiron Corporation, 4560 Horton Street, Mail Stop 4.3, Emeryville, CA 94608, USA. gillis_otten@chiron.com
Chen
Minchao
M
Doe
Barbara
B
zur Megede
Jan
J
Barnett
Susan
S
Ulmer
Jeffrey
J
D016915:AIDS Vaccines / Q000235:genetics / Q000276:immunology*; D019264:Adoptive Transfer; D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D015750:Genes, gag / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D051379:Mice; D013154:Spleen / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology
D016915:AIDS Vaccines; D001426:Bacterial Proteins; C113709:HsvG protein, Pantoea agglomerans; D014614:Vaccines, Synthetic; D007371:Interferon-gamma