[Characteristics of field isolates of Newcastle disease virus isolated in the course of outbreaks in the poultry plant in the Leningrad region in 2000].
A field isolate of Newcastle disease virus (NDV) was isolated in the Russko-Vysotskaya poultry farm, Leningrad region. Within four days after infection, the isolate caused 100% mortality in 60-day-old susceptible chickens. The HA titer of the allantoic fluid samples collected after one passage in SPF-chicken embryos was 1:512, and it reacted only with the NDV specific antiserum in HI test. Intracerebral pathogenicity index and mean embryo death time were 1.97 and 49 hours, respectively. The isolate has the amino acid sequence of the protease cleavage site of the fusion protein F0 (112R-R-Q-R-R-F117), which is similar to that in the velogenic strains of NDV. Therefore, it was concluded that the virus isolated in this work was an ethiological agent of the ND outbreak in this poultry farm.
Manin
T B
TB
Shcherbakova
L O
LO
Bochkov
Iu A
IuA
El'nikov
V V
VV
Pchelkina
I P
IP
Starov
S K
SK
Drygin
V V
VV
D000818:Animals; D020134:Catalytic Domain / Q000235:genetics; D002642:Chick Embryo; D002645:Chickens; D004196:Disease Outbreaks / Q000662:veterinary*; D010450:Endopeptidases / Q000378:metabolism; D006386:Hemagglutination Tests; D009521:Newcastle Disease / Q000453:epidemiology* / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011200:Poultry / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis; D012426:Russia / Q000453:epidemiology; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism
D012367:RNA, Viral; D014760:Viral Fusion Proteins; D010450:Endopeptidases