[Genetic typing of Mycobacterium tuberculosis strains by spoligotyping and genome fingerprinting techniques].
A total of 122 M. tuberculosis clinical drug-resistant strains isolated in Central Russia were studied by spoligotyping and genome fingerprinting techniques. According to spoligotyping results 77% of M. tuberculosis strains were distributed to 13 oligotypes, while 23% of these strains were found to form unique patterns. Most of them belonged to the families Beijing and Haarlem (43.4% and 13.9% respectively). The patterns of the strains of oligotype 12 (7F-7F-7E-0E-78-3E) were identical to those of the strains isolated in Brazil, France and the Netherlands. The strains of the spoligotype 22 (7F-1E-7F-7F-07-3E) had the patterns identical to those of the strains of group S13, also isolated in Brazil. According to genome fingerprinting 31.4% of the strains were found to belong to clusters with the similarity coefficient equal to 1. The strains belonging to genotypes W and A1 were found to prevail in the analyzed group.
Shemiakin
I G
IG
State Research Center of Applied Microbiology, Obolensk, Moscow Region, Russia.
Stepanshina
V N
VN
Korobova
O V
OV
Anisimova
V A
VA
Ivanov
I Iu
IIu
Lipin
M Iu
MIu
Lazarev
A  A
A
Skaldina
A I
AI
Chernavskaia
L A
LA
Tarasova
T I
TI
D000995:Antitubercular Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D005838:Genotype; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D009841:Oligonucleotides / Q000032:analysis; D012150:Polymorphism, Restriction Fragment Length; D021521:Ribotyping / Q000379:methods*; D012426:Russia; D013045:Species Specificity; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology*; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology
D000995:Antitubercular Agents; D004269:DNA, Bacterial; D009841:Oligonucleotides