title: Salt stress sensitivity of nitrogen fixation in Enterobacter agglomerans strains.
abstract: Two strains 333 to 339 of Enterobacter agglomerans were selected in the present study to evaluate the response of increasing concentrations of NaCl on growth, N(2)-fixation, and nitrogenase activity/synthesis. E. agglomerans strains 333 and 339 showed optimum growth and acetylene-reducing activity with 0.5 to 1.0% NaCl in a nitrogen-free minimal medium (NFDM) with glucose, respectively, in 28 h incubation, although both strains displayed better growth and acetylene-reducing activity with 3.0% and 2.0% NaCl after 52 h and 100 h incubation periods than the 28 h culture did. Our experiments with shiftings of salt concentrations in NFDM medium indicated that a synthesis of nitrogenase enzyme was generally more sensitive to higher concentrations of NaCl than nitrogenase activity was.
authors.affiliation: Department of Genetics, University of Bayreuth, D-95440 Bayreuth, Germany.
authors.forename: Raman
authors.initials: R
authors.lastname: Rai
authors.forename: Gabriele
authors.initials: G
authors.lastname: Rieder