[The experimental study of intracellular actin in the scar fibroblasts].
OBJECTIVE
To study the relationship between intracellular actin and scar contracture.


METHODS
Fibroblasts from 10 cases of hypertrophic scar and 5 cases of keloid were cultured in vitro. Total actin, filamentous actin(F actin), globular actin (G actin) and the ratio of F to G actin(F/G) were measured by densitometry after differential extraction and separation by polyacrylamide gel electrophoresis in the presence of sodium sulfate.


RESULTS
Total actin, F actin, G actin and F/G in hypertrophic scar fibroblasts were 2.38 ng/10(4) cells, 0.98 ng/10(4) cells, 1.42 ng/10(4) cells and 0.68 respectively, while in keloid fibroblasts were 1.68 ng/10(4) cells. 0.46 ng/10(4) cells, 1.26 ng/10(4) cells, and 0.36 respectively. There was significant differences between two tissues fibroblasts in the items of total actin, F actin, G actin, and F/G (P < 0.01), while no significant difference in G actin (P > 0.05).


CONCLUSIONS
Total intracellular actin, F actin, and F/G may play an important role in the scar contracture. The hypertrophic scar and keloid can be distinguished by the contents of total intracellular actin, F actin and F/G.
Zhang
Q
Q
Department of Plastic Surgery, Ninth People's Hospital, Shanghai Second Medical University, Shanghai, P.R China 200011.
Cui
L
L
Qian
Y L
YL
D000199:Actins / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D017439:Cicatrix, Hypertrophic / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007627:Keloid / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
D000199:Actins