title: [Cloning and sequencing of adenovirus type 7 vaccine strain 76.5-87 mu fragment].
abstract: Adenovirus 7(Ad7) vaccine strain has been used more than 30 years in US army recruits for prevention of Ad7 infection and has been proven to be safe and effective. Recent years more attention has been paid to exploring the possibilities of using it for gene therapy or making recombinant vaccine. In order to construct adenovirus 7 vector, Ad 7 vaccine strain 76.5-87 mu fragment was cloned and sequenced. The fragment contains 3,557 bp which encodes 6 proteins of E3 region, namely 12.1 kD, 19.2 kD, 20.1 kD, 20.5 kD, 10.3 kD and partial of 15.2 kD. Nucleotide sequences of all these proteins show a high homology with the corresponding region of human adenovirus prototype strain Ad 7(Ad 7p) and Ad 7h(the homology of nucleotide sequence of the 6 proteins is more than 97.2%). The equivalent of ORF 16.1 kD in Ad7p and Ad7h was mutation due to a base pair deletion. The region corresponding to ORF 7.7 kD in Ad7p was missing in Ad7v due to a deletion. The results should be helpful to the construction of Ad7v vectors.
authors.affiliation: Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052.
authors.forename: C
authors.initials: C
authors.lastname: Shi
authors.forename: L
authors.initials: L
authors.lastname: Wen
authors.forename: S
authors.initials: S
authors.lastname: Liu
mesh_terms: D017503:Adenovirus E3 Proteins / Q000235:genetics*; D000260:Adenoviruses, Human / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA; D014614:Vaccines, Synthetic
chemical_list: D017503:Adenovirus E3 Proteins; D004279:DNA, Viral; D014614:Vaccines, Synthetic