title: Distribution of microorganisms in the subsurface of the manus basin hydrothermal vent field in Papua New Guinea.
abstract: The distribution of microorganisms in the subsurfaces of hydrothermal vents was investigated by using subvent rock core samples. Microbial cells and ATP were detected from cores taken at depths of less than 99.4 and 44.8 m below the seafloor (mbsf), respectively. Cores from various depths were incubated anaerobically with a heterotrophic medium. Growth at 60 and 90 degrees C was ascribed to a Geobacillus sp. in the 448.6- to 99.4-mbsf cores and a Deinococcus sp. in the 64.8- to 128.9-mbsf cores, respectively, based on the 16S ribosomal DNA analysis.
authors.affiliation: School of Biosphere Sciences, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 739-8528, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kimura
authors.forename: Ryuji
authors.initials: R
authors.lastname: Asada
authors.forename: Andrew
authors.initials: A
authors.lastname: Masta
authors.forename: Takeshi
authors.initials: T
authors.lastname: Naganuma
mesh_terms: D000693:Anaerobiosis; D001406:Bacillaceae / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D003470:Culture Media; D004275:DNA, Ribosomal / Q000032:analysis; D034301:Deinococcus / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D019015:Geologic Sediments / Q000382:microbiology*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010219:Papua New Guinea; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D003470:Culture Media; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S