title: [Study on the intra- and extra-hepatocyte distribution of sodium ions in scalded rats during early postburn stage].
abstract: OBJECTIVE
  To investigate the characteristics of the intra- and extra-hepatocyte sodium ions distribution in scalded rats during early postburn stage,with the aim of improving burn shock resuscitation regime and the resuscitation effects.
  
  
  METHODS
  Adult Sprague-Dawley rats were randomly divided into sham scalding (C, n = 12) and scalding (S, n = 7) groups. The rats in S group were subjected to 40% TBSA III degree scalding on the back and were catheterized via jugular vein for fluid resuscitation. The rats in C group were catheterized via jugular vein without fluid infusion and were sham scalded by warm water in temperature of 37 degrees. The changes in the intra- and extra-hepatocyte sodium ion contents were determined in vivo by (23)Na-magnetic resonance spectrum technology, while the existing state of the intra- and extra-hepatocyte sodium ion was determined by detecting (23)Na-magnetic resonance horizontal delaying time (T(2)).
  
  
  RESULTS
  The extra-hepatocyte sodium content in S group at 24 postburn hours (PBHs) was 17% less than that in C group. In addition, the T(2f) (fast T(2)) in S group remained stable but maintained a higher ratio during the observation time. This suggested that the sodium binding sites in extra-hepatocyte matrix increased relatively and that intra-hepatocyte sodium content increased by 57%. But the T(2) and the fast and slow parts of the T(2) kept stable, which implied that intra-hepatocyte catabolizing products were increased. This led to an increase in the sodium ion binding sites within intra-hepatocyte matrix in proportion to the sodium ion content.
  
  
  CONCLUSIONS
  During early postburn stage, the extra-hepatocyte sodium in a remote organ such as the liver exhibited relative deficiency due to its ingress into hepatocyte cytoplasm and to the increase of sodium combining sites.
authors.affiliation: Department of Burns, Chang Hai Hospital, The Second Military Medical University, Shanghai, 200433. P.R. China.
authors.forename: Zhaofan
authors.initials: Z
authors.lastname: Xia
authors.forename: Guangyi
authors.initials: G
authors.lastname: Wang
authors.forename: Hongtai
authors.initials: H
authors.lastname: Tang
authors.forename: Shihui
authors.initials: S
authors.lastname: Zhu
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Duo
authors.initials: D
authors.lastname: Wei
authors.forename: Jingning
authors.initials: J
authors.lastname: Huan
authors.forename: Shengde
authors.initials: S
authors.lastname: Ge
mesh_terms: D000818:Animals; D001665:Binding Sites; D002056:Burns / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism*
chemical_list: D012964:Sodium