title: Crystallization and preliminary crystallographic analysis of a partial extracellular fragment of a sperm membrane protein YWK-II/APPH related to the Alzheimer betaA4-amyloid precursor protein.
abstract: Crystals of a partial extracellular fragment of a human sperm membrane protein YWK-II/APPH have been grown at 291 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.8 A resolution at 100 K using Cu K(alpha) radiation. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 46.009, b = 67.387, c = 149.241 A, alpha = beta = gamma = 90 degrees. The presence of two molecules per asymmetric unit gives a crystal Volume per protein mass (V(M)) of 3.51 A(3) Da(-1) and a solvent content of 64.6% by Volume. A full set of X-ray diffraction data were collected to 2.8 A resolution from the native crystal.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, People's Republic of China.
authors.forename: Maojun
authors.initials: M
authors.lastname: Yang
authors.forename: Binbin
authors.initials: B
authors.lastname: Liu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.forename: Weihong
authors.initials: W
authors.lastname: Zhou
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Hai
authors.initials: H
authors.lastname: Pang
authors.forename: Shiying
authors.initials: S
authors.lastname: Miao
authors.forename: Linfang
authors.initials: L
authors.lastname: Wang
authors.forename: Zihe
authors.initials: Z
authors.lastname: Rao
mesh_terms: D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000235:genetics; D002462:Cell Membrane / Q000737:chemistry; D017124:Conserved Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments / Q000737:chemistry*; D013094:Spermatozoa / Q000737:chemistry*
chemical_list: C489697:APLP1 protein, human; C084104:APLP2 protein, human; D016564:Amyloid beta-Protein Precursor; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments