title: Prediction of fetal Rh D and Rh CcEe phenotype from maternal plasma with real-time polymerase chain reaction.
abstract: Real-time PCR methods for the detection of RHD and the C, c, and E allele of RHCE were applied for the prediction of fetal Rh phenotype using maternal plasma. In one of 36 samples investigated the DNA extraction failed. When we tested the remaining 35 samples for Rh antigens which were absent on the mother's red cells, the fetal D-status was correctly determined in 26 of 27 cases (1 false negative). Fetal C was tested correctly in 23 samples, c was true positive in the only c-negative woman and the fetal E-status was correctly determined in 35 cases. In conclusion real-time PCR of maternal plasma is a non-invasive method to determine fetal RH genotype. However, more studies are required for routine applications because the method is not 100% sensitive.
authors.affiliation: Department of Transfusion Medicine, University of Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany. tlegler@med.uni-goettingen.de
authors.forename: Tobias J
authors.initials: TJ
authors.lastname: Legler
authors.forename: Rainer
authors.initials: R
authors.lastname: Lynen
authors.forename: Jens-Holger
authors.initials: JH
authors.lastname: Maas
authors.forename: Gerhard
authors.initials: G
authors.lastname: Pindur
authors.forename: Dietrich
authors.initials: D
authors.lastname: Kulenkampff
authors.forename: Anette
authors.initials: A
authors.lastname: Suren
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Osmers
authors.forename: Michael
authors.initials: M
authors.lastname: Köhler
mesh_terms: D000483:Alleles; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D003199:Computer Systems; D004247:DNA / Q000097:blood* / Q000302:isolation & purification; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D005331:Fetomaternal Transfusion; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007231:Infant, Newborn; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D044469:Racial Groups / Q000235:genetics; D012189:Retrospective Studies; D012203:Rh Isoimmunization / Q000517:prevention & control; D012204:Rh-Hr Blood-Group System / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D006023:Glycoproteins; C066468:RHCE protein, human; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen; D004247:DNA