title: Analysis of catecholamines by capillary electrophoresis and capillary electrophoresis-nanospray mass spectrometry. Use of aqueous and non-aqueous solutions compared with physical parameters.
abstract: Catecholamines were analysed in aqueous and alcoholic non-aqueous solutions by capillary electrophoresis and capillary electrophoresis-mass spectrometry using sheathless nanospray coupling. Decreases in the electrophoretic mobilities of the catecholamines and in the electroosmotic mobilities were observed from water to 1-propanol. Separations were more efficient in all non-aqueous media than in water. The diffusion coefficients of the catecholamines in the different media were determined. The solvent had little effect on the sensitivity of the UV or MS detection. Both methods were successfully applied to the analysis of urine samples.
authors.affiliation: University of Helsinki, Department of Pharmacy, Viikki Drug Discovery Technology Center (DDTC), P.O. Box 56, FIN-00014 Helsinki, Finland
authors.forename: Katariina
authors.initials: K
authors.lastname: Vuorensola
authors.forename: Heli
authors.initials: H
authors.lastname: Sirén
authors.forename: Risto
authors.initials: R
authors.lastname: Kostiainen
authors.forename: Tapio
authors.initials: T
authors.lastname: Kotiaho
mesh_terms: D002395:Catecholamines / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D014867:Water
chemical_list: D002395:Catecholamines; D012996:Solutions; D014867:Water