Fc epsilon RI and CD22 mRNA are expressed in early B-lineage and myeloid leukemia cell lines.
CD22, one of the important markers for diagnosing B-lineage acute leukemia, was expressed in mature basophil granulocytes. We then investigated the expression of CD22 and other B cell- and basophil-related molecules in 25 human acute leukemia cell lines to find the phenotype of the virtual common progenitor of B and myeloid lineage. Surface and cytoplasmic expressions of antigens were analyzed using a flow cytometer and an essential antibody panel used for diagnosing acute leukemia as well as cytokine receptors and basophil-related enzymes. Messenger RNA expression of Fc epsilon R1 and CD22 was also analyzed. Peroxidase-positive and -negative myeloid leukemias showed eosinophil- and basophil-type expression of enzymes, respectively. Early myeloid and B-lineage cells expressed basically similar combinations of cytokine receptors and various combinations of mRNA listed above, while T-lineage cells did not. The virtual common progenitor of B and myeloid lineage cells may be defined as immature cells simultaneously expressing B and basophil phenotypes.
Toba
Ken
K
First Department of Internal Medicine, Niigata University Medical Hospital, Niigata City, Japan. tobaken@med.niigata-u.ac.jp
Hanawa
Haruo
H
Sakaue
Minori
M
Yoshida
Kaori
K
Itoh
Hiromi
H
Tsuchiyama
Junjiro
J
Maruyama
Soichi
S
Narita
Miwako
M
Takahashi
Masuhiro
M
Watanabe
Kenichi
K
Aizawa
Yoshifusa
Y
D000208:Acute Disease; D015703:Antigens, CD / Q000235:genetics*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics*; D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D001491:Basophils / Q000166:cytology / Q000378:metabolism; D015815:Cell Adhesion Molecules; D019070:Cell Lineage; D006801:Humans; D016130:Immunophenotyping; D037102:Lectins / Q000235:genetics*; D007938:Leukemia / Q000473:pathology; D007951:Leukemia, Myeloid / Q000473:pathology*; D023461:Myeloid Progenitor Cells / Q000166:cytology; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D017455:Receptors, IgE / Q000235:genetics*; D051918:Sialic Acid Binding Ig-like Lectin 2; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; C497867:CD22 protein, human; D015815:Cell Adhesion Molecules; D037102:Lectins; D012333:RNA, Messenger; D017455:Receptors, IgE; D051918:Sialic Acid Binding Ig-like Lectin 2