Evaluation of the role of P-glycoprotein in inflammation induced blood-brain barrier damage.
In this study we evaluated the role of the multi-drug transporter p-glycoprotein (Pgp) in the process of activated T lymphocyte-mediated blood-brain barrier dysfunction as described previously. Lymphocyte exposure induced significant endothelial cell death and there was an elevation of the expression of Pgp in the surviving cells. Inhibition of Pgp function using the antibody MRK16 and verapamil displayed a dose-dependent prevention of T cell mediated endothelial cell death and barrier breakdown. These data suggest that the activity of Pgp at the blood-brain barrier may play a role in lymphocyte induced barrier cell damage and a role as a possible survival mechanism to prevent further endothelial cell death in later stages of inflammation.
Tan
Kian H
KH
Department of Neurochemistry, Institute of Neurology, Univeristy College London, London WC1N 3BG, UK.
Purcell
Wendy M
WM
Heales
Simon J R
SJ
McLeod
Julie D
JD
Hurst
Roger D
RD
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000276:immunology*; D000818:Animals; D001812:Blood-Brain Barrier / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology; D016923:Cell Death / Q000276:immunology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology*; D005910:Glioma; D006801:Humans; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins / Q000166:cytology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1