title: Structure and nucleotide sequence of Euphorbia characias copper/TPQ-containing amine oxidase gene.
abstract: A cDNA encoding for a copper containing amine oxidase has been isolated and sequenced from young leaves of Euphorbia characias, a perennial mediterranean shrub. A single long open reading frame of 2068 pb encodes a protein composed of 653 amino acids with a molecular mass of about 74 kDa. A putative 24-aminoacid signal peptide precedes the sequence of the mature protein, with characteristics of a secretion signal peptide. Alignments of Euphorbia amine oxidase cDNA nucleotide sequence with that of amine oxidase from the seedlings of the pulses lentil, pea, and chickpea reveal several conserved regions, especially in the C-terminus, with a homology 90%-97%. The near 5' region shows several insertions, deletions, and different nucleotide sequence with ca. 60% homology. The enzyme contains 1%-2% carbohydrate deduced by deglycosylation experiments. Five cysteine residues are present in the deduced aminoacid sequence with a single disulfide bridge as judged by titration with cysteine reagents.
authors.affiliation: Department of Sciences Applied to Biosystems, University of Cagliari, Cagliari, Italy. padiglia@unica.it
authors.forename: Alessandra
authors.initials: A
authors.lastname: Padiglia
authors.forename: Rosaria
authors.initials: R
authors.lastname: Medda
authors.forename: Tiziana
authors.initials: T
authors.lastname: Scanu
authors.forename: Silvia
authors.initials: S
authors.lastname: Longu
authors.forename: Antonello
authors.initials: A
authors.lastname: Rossi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Floris
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D004220:Disulfides / Q000737:chemistry; D028481:Euphorbia / Q000201:enzymology* / Q000235:genetics; D007887:Fabaceae / Q000235:genetics; D006031:Glycosylation; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D018515:Plant Leaves / Q000201:enzymology; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018076:DNA, Complementary; D004220:Disulfides; D006631:Amine Oxidase (Copper-Containing)