[The effect of separating red blood cells from bone marrow graft in vitro by methylcellulose].
It has need to separate red blood cells (RBC) from marrow graft in ABO group unmatched BMT and auto-BMT with purging tumor cells, the separating effect of methylcellulose was observed. The mixture of 0.5% methylcellulose and bone marrow was laid up in an open transfusion system, and then sedimentation of RBC was performed in the transfusion tube. The separating results of 18 marrow grafts showed that the recovery rates of mononuclear cells and CD34(+) cells were (83.8 +/- 55.2)% and (90.3 +/- 7.2)%, respectively. RBC residual rate was (4.3 +/- 1.5)%. The yield of CFU-GM was (60.8 +/- 22.4)/2 x 10(5) MNC, and there was no difference to [(69.8 +/- 23.4)/2 x 10(5) MNC] yielded from same marrow samples, separated by Ficoll-Hypaque separation. It is concluded that this method could be used for bone marrow transplantation.
Zhang
Miao
M
Department of Hematology, The General Hospital of PLA, Beijing 100853, China. miao-zhang@hotmail.com
Zhang
Bo-Long
BL
Jin
Hai-Jie
HJ
Gao
Chun-Ji
CJ
Lou
Fang-Ding
FD
Da
Wan-Ming
WM
D016026:Bone Marrow Transplantation / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D008747:Methylcellulose / Q000494:pharmacology*
D008747:Methylcellulose