title: Taenia saginata derived synthetic peptides with potential for the diagnosis of bovine cysticercosis.
abstract: Immunity in Taeniids is predominantly antibody mediated and thus many serological immuno-determinants will have potential in both protection and diagnosis. The antigenicity of six peptides derived from four potentially protective molecules cloned from a Taenia saginata oncospheres cDNA library have been evaluated as targets for the specific diagnosis of bovine cysticercosis. The six peptides consist of: two peptides (HP6-2 and HP6-3) derived from the sequence of the 18 kDa surface/secreted oncospheral adhesion antigen identified by McAb-HP6, two peptides (Ts45W-1 and Ts45W-5) derived from the sequence of the T. saginata homologue of the T. ovis 45W protective gene family, one peptide (TS45S-10) derived from a T. saginata sequence with significant similarity to the T. ovis 45S protective antigen, and one peptide (TEG-1) derived from the sequence of the T. saginata homologue of Echinococcus spp. main surface protein. Longitudinal studies indicate that T. saginata infected cattle respond to all six peptides by 3-4 weeks post-infection and that the antibody levels remain high for at least 12 weeks post-infection. As protection against Taeniid parasites is predominantly antibody mediated, some of these six peptides may be of value as immuno-prophylactic tools and hence also in assays to determine resistance to infection with the parasite. For diagnosis, on the other hand, only three peptides (HP6-2, TEG-1 and Ts45S-10) performed with the necessary sensitivity and specificity to determine exposure to infection with T. saginata, and now merit an exhaustive evaluation prior to employment as routine diagnostic tools.
authors.affiliation: Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey GU24 ONF, UK.
authors.forename: E
authors.initials: E
authors.lastname: Ferrer
authors.forename: L
authors.initials: L
authors.lastname: Benitez
authors.forename: M
authors.initials: M
authors.lastname: Foster-Cuevas
authors.forename: D
authors.initials: D
authors.lastname: Bryce
authors.forename: L W
authors.initials: LW
authors.lastname: Wamae
authors.forename: J A
authors.initials: JA
authors.lastname: Onyango-Abuje
authors.forename: T
authors.initials: T
authors.lastname: Garate
authors.forename: L J S
authors.initials: LJ
authors.lastname: Harrison
authors.forename: R M E
authors.initials: RM
authors.lastname: Parkhouse
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D003429:Cross Reactions; D003551:Cysticercosis / Q000175:diagnosis* / Q000276:immunology / Q000662:veterinary*; D005260:Female; D007106:Immune Sera / Q000276:immunology; D008297:Male; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D041202:Taenia saginata / Q000276:immunology*; D013997:Time Factors
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007106:Immune Sera; D010455:Peptides