title: [Protective effect of bajitian oligosaccharide on PC12 cells lesioned by corticosterone].
abstract: OBJECTIVE
  To study the possible mechanism of antidepressant effect of Bajitian oligosaccharide (MW-97).
  
  
  RESULTS
  After incubating with the PC12 cells in the presence of corticosterone(2 x 10(-4) mol.L-1) for 48 h, it was found that MW-97 could protect PC12 cells from the lesion done by corticosterone in a concentration-dependent manner. Furthermore, using RT-PCR mediated cross-species partial cDNA cloning, it was found that MW-97 and desipramine(DIM) increased NGF, BDNF mRNA in the frontal cortex after chronic administration for 21 days, meanwhile, BDNF mRNA in hippocampus was also increased.
  
  
  CONCLUSIONS
  The cytoprotective effect of MW-97 consists in the possible mechanism of its antidepressant action, while the increasing expression of neurotrophic factors (NGF, BDNF mRNA) may be contributes to the cytoprotective effect.
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.forename: Y M
authors.initials: YM
authors.lastname: Zhao
authors.forename: Z P
authors.initials: ZP
authors.lastname: Luo
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000302:isolation & purification / Q000494:pharmacology; D001921:Brain / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D020932:Nerve Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology; D009844:Oligosaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D016716:PC12 Cells / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D019666:Rubiaceae / Q000737:chemistry*
chemical_list: D000928:Antidepressive Agents; D019208:Brain-Derived Neurotrophic Factor; D018696:Neuroprotective Agents; D009844:Oligosaccharides; D012333:RNA, Messenger; D020932:Nerve Growth Factor