A simple method for preparation of D-rhamnose.
A rapid procedure for the preparation of D-rhamnose from bacterial lipopolysaccharide (LPS) has been developed. It involves purification of LPS from Pseudomonas syringae pv. phaseolicola by phenol extraction and hydrophobic interaction chromatography (HIC), followed by mild hydrolysis and cleavage of the O-antigen into D-fucose and D-rhamnose. The monosaccharides were separated by column chromatography, and D-rhamnose recovered after filtration over Sephadex-LH 20.
Ramm
Michael
M
Institute of Pharmacy, Friedrich-Schiller-University Jena, Semmelweisstrasse 10, D-07743 Jena, Germany. mramm@pmail.hki-jena.de
Lobe
Martina
M
Hamburger
Matthias
M
D002845:Chromatography; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification; D011549:Pseudomonas / Q000737:chemistry; D012210:Rhamnose / Q000138:chemical synthesis* / Q000302:isolation & purification
D008070:Lipopolysaccharides; D012210:Rhamnose