Inhibitory effects of the root cortex of Paeonia suffruticosa on interleukin-8 and macrophage chemoattractant protein-1 secretions in U937 cells.
In an effort to elucidate the mechanism of the anti-inflammatory effect of mudanpi, the root cortex of Paeonia suffruticosa Andrews (Ranunculaceae), we determined the effects of the methanolic extract of mudanpi (MEM) on the secretions of interleukin (IL)-8, a major mediator of acute neutrophil-mediated inflammation, and macrophage chemoattractant protein (MCP)-1, a major mediator of chronic macrophage-mediated inflammation, in human monocytic U937 cells stimulated with phorbol myristate acetate (PMA). MEM significantly inhibited PMA-induced secretions of IL-8 and MCP-1 proteins in a dose-dependent manner. The inhibition of these chemokines by MEM was due to its suppression of IL-8 and MCP-1 genes. In addition, 1,2,3,4,6-penta-O-galloyl-beta-D-glucose, one of major constituents isolated from MEM, inhibited PMA-induced secretions of IL-8 and MCP-1 proteins by its suppression of IL-8 and MCP-1 genes. Thus, one possible anti-inflammatory mechanism of mudanpi, an anti-inflammatory Chinese crude drug, may be to inhibit the secretions of inflammatory chemokines.
Oh
G S
GS
Department of Microbiology and Immunology, Medicinal Resources Research Center of Wonkwang University, Wonkwang University School of Medicine, 344-2 Shinyong-Dong, Iksan-Shi, Chonbuk 570-749, South Korea.
Pae
H O
HO
Choi
B M
BM
Jeong
S
S
Oh
H
H
Oh
C S
CS
Rho
Y D
YD
Kim
D H
DH
Shin
M K
MK
Chung
Hun-Taeg
HT
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D008518:Medicine, East Asian Traditional; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D029596:Paeonia / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D020298:U937 Cells
D000893:Anti-Inflammatory Agents; D018932:Chemokine CCL2; D016209:Interleukin-8; D010936:Plant Extracts; D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate