title: Endometrial function in vervet monkeys (Cercopithecus aethiops): morphology, beta3 integrin and insulin-like growth factor binding protein-1 expression during the menstrual cycle and pregnancy in the normal and disrupted endometrium.
abstract: The expression of endometrial beta3 integrin and insulin-like growth factor binding protein-1 (IGFBP-1) was studied in cycling and pregnant vervet monkeys. There were clear changes of beta3 integrin expression during the menstrual cycle, with the strongest immunostaining observed on day 26. Moderate to strong expression was observed during pregnancy. The expression of IGFBP-1 during the menstrual cycle was weak but upregulated during pregnancy with moderate to strong staining. The administration of a single dose of onapristone at 10 mg/kg on days 17, 21 and 22 of the menstrual cycle, followed by a biopsy on days 22, 22 and 26, respectively, and during pregnancy (34-44 days menstrual age) 24 h before the biopsy, disrupted and desynchronized the endometrium. However, no effect on beta3 integrin expression could be observed and staining reflected the untreated patterns. The same applied to IGFBP-1 except that during pregnancy the expression of this protein was reduced or abolished. The results suggest that beta3 integrin is associated with endometrial receptivity in vervet monkeys and that IGFBP-1 plays an important role during pregnancy in this species. The administration of onapristone appeared to only influence IGFBP-1 expression. To our knowledge, this is the first time that these endometrial proteins have been investigated in vervet monkeys. This study should therefore contribute to improving our understanding of the reproductive function of this species.
authors.affiliation: Primate Unit, Diabetes Research Group (formerly Experimental Biology Programme)/MRC, Tygerberg, Cape Town, South Africa. jurgun.seier@mrc.ac.za
authors.forename: J V
authors.initials: JV
authors.lastname: Seier
authors.forename: K
authors.initials: K
authors.lastname: Chwalisz
authors.forename: J
authors.initials: J
authors.lastname: Louw
authors.forename: G
authors.initials: G
authors.lastname: van der Horst
authors.forename: M
authors.initials: M
authors.lastname: de Kock
authors.forename: D
authors.initials: D
authors.lastname: du Toit
authors.forename: J A
authors.initials: JA
authors.lastname: Laubscher
mesh_terms: D000818:Animals; D001706:Biopsy / Q000662:veterinary; D002522:Chlorocebus aethiops / Q000033:anatomy & histology / Q000378:metabolism / Q000502:physiology*; D004717:Endometrium / Q000033:anatomy & histology / Q000378:metabolism / Q000502:physiology*; D005260:Female; D006067:Gonanes / Q000494:pharmacology; D006727:Hormone Antagonists / Q000494:pharmacology; D007150:Immunohistochemistry / Q000662:veterinary; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000096:biosynthesis* / Q000378:metabolism; D039661:Integrin beta3 / Q000096:biosynthesis* / Q000378:metabolism; D008297:Male; D008597:Menstrual Cycle / Q000502:physiology*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism / Q000502:physiology*; D011374:Progesterone / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D006067:Gonanes; D006727:Hormone Antagonists; D018970:Insulin-Like Growth Factor Binding Protein 1; D039661:Integrin beta3; D011374:Progesterone; C053238:onapristone