Overexpression and RNA interference of Ap-cyclic AMP-response element binding protein-2, a repressor of long-term facilitation, in Aplysia kurodai sensory-to-motor synapses.
cyclic AMP-response element binding protein-2 (CREB2) is a member of the CREB/transcription factor (CREB/ATF4) family. CREB2 is a transcription factor known to be involved in Aplysia long-term facilitation. To further examine the role of ApCREB2 on long-term synaptic facilitation, we isolated ApCREB2 from Aplysia kurodai in full-length cDNA library, and found that the overexpression of ApCREB2 blocked 5-hydroxytryptamine (5-HT)-induced long-term synaptic facilitation in Aplysia sensory-to-motor synapses. Furthermore, a single pulse of 5-HT, which normally induces only short-term facilitation, in the presence of ApCREB2 inhibition by RNA interference, induced long-term facilitation in Aplysia sensory-to-motor synapses. These results suggest that ApCREB2 is a functional repressor of long-term facilitation in Aplysia sensory-to-motor synapses.
Lee
Jin-A
JA
National Research Laboratory, Institute of Molecular Biology and Genetics, School of Biological Sciences, College of Natural Sciences, Seoul National University, South Korea.
Kim
Hyoung
H
Lee
Yong-Seok
YS
Kaang
Bong-Kiun
BK
D000818:Animals; D001048:Aplysia; D002478:Cells, Cultured; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics / Q000378:metabolism*; D019706:Excitatory Postsynaptic Potentials; D017774:Long-Term Potentiation / Q000187:drug effects; D018613:Microscopy, Confocal; D009046:Motor Neurons / Q000502:physiology / Q000648:ultrastructure; D009475:Neurons, Afferent / Q000502:physiology / Q000648:ultrastructure; D012313:RNA / Q000378:metabolism*; D012701:Serotonin / Q000494:pharmacology; D013569:Synapses / Q000378:metabolism / Q000502:physiology*
D017362:Cyclic AMP Response Element-Binding Protein; D012701:Serotonin; D012313:RNA