Effect of allopurinol, superoxide-dismutase, and hyperbaric oxygen on flap survival.
The effect of allopurinol, superoxide-dismutase, and hyperbaric oxygen was compared on axial pattern skin flap survival. An abdominal flap based on the inferior epigastric pedicle was raised in rats for this purpose. Three experimental groups were studied. In the first group, adult male Wistar rats received 50 mg/kg I.P. of allopurinol. The second received 20,000 I.U./kg of superoxide-dismutase. The third group was submitted to hyperbaric oxygen therapy. The flaps were exposed to 8-h warm ischemia. Flap survival was evaluated on postoperative day 7. All flaps survived, and the mean survival areas were 63.53%, 83.03%, and 55.98%, respectively, in the allopurinol, superoxide-dismutase, and hyperbaric groups. The percentage of flap necrosis was significantly smaller in all experimental groups when compared to controls (P < 0.05). It was clear that the superoxide-dismutase group had better results on axial pattern ischemic skin flap survival, under the tested conditions. The tested methods improved flap survival to ischemic injury, and the flap designed is a reliable model for further investigations.
Prada
Flavia S
FS
Section of Reconstructive Microsurgery, Division of Traumatology and Orthopaedic Surgery, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil. fsprada@unisys.com.br
Arrunategui
Gino
G
Alves
Marcos C
MC
Ferreira
Marcos C
MC
Zumiotti
Arnaldo V
AV
D000005:Abdomen / Q000601:surgery; D000493:Allopurinol / Q000494:pharmacology*; D000818:Animals; D062109:Dermatologic Surgical Procedures; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006085:Graft Survival / Q000187:drug effects*; D006931:Hyperbaric Oxygenation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects; D016038:Skin Transplantation; D013482:Superoxide Dismutase / Q000494:pharmacology*; D013524:Surgical Flaps; D013997:Time Factors
D016166:Free Radical Scavengers; D000493:Allopurinol; D013482:Superoxide Dismutase