Effect of multiple freeze-thaw cycles on detection of measles, mumps, and rubella virus antibodies.
We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4 degrees C until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on the ability to measure these antibodies by enzyme-linked immunoassays.
Pinsky
Norman A
NA
Mayo Vaccine Research Group, Mayo Clinic, Rochester, Minnesota 55905, USA.
Huddleston
Jeanne M
JM
Jacobson
Robert M
RM
Wollan
Peter C
PC
Poland
Gregory A
GA
D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D015925:Cryopreservation; D004797:Enzyme-Linked Immunosorbent Assay; D005615:Freezing; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008457:Measles / Q000175:diagnosis / Q000276:immunology; D009107:Mumps / Q000175:diagnosis / Q000276:immunology; D012327:RNA Virus Infections / Q000175:diagnosis* / Q000276:immunology; D015203:Reproducibility of Results; D012409:Rubella / Q000175:diagnosis / Q000276:immunology
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M