The use of laser microdissection for the preparation of chromosome-specific painting probes in farm animals.
Laser microbeam microdissection and laser pressure catapulting procedure were used for the construction of chromosome-specific painting probes, arm-specific probes and probes for chromosomal subfragments. We report on a method for generation of fluorescence in-situ hybridization probes from laser dissected chromosomes of farm animals. So far, using the described method, a set of chromosome-specific painting probes has been obtained for all porcine chromosomes, 17 chromosomes of cattle and selected equine chromosomes. It is concluded that the laser technology appears to be a useful and powerful tool for the construction of chromosome-specifi c painting probes. Its main advantage is the fast non-contact collection of chromosomes.
Kubickova
Svatava
S
Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic.
Cernohorska
Halina
H
Musilova
Petra
P
Rubes
Jiri
J
D000818:Animals; D000829:Animals, Domestic / Q000235:genetics*; D002417:Cattle; D002871:Chromosome Banding; D020223:Chromosome Painting / Q000379:methods*; D002875:Chromosomes / Q000235:genetics*; D015342:DNA Probes; D004210:Dissection / Q000379:methods*; D018397:Equidae; D005819:Genetic Markers; D006579:Heterozygote; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D007834:Lasers; D013045:Species Specificity; D013552:Swine; D019914:Taq Polymerase / Q000235:genetics; D014178:Translocation, Genetic
D015342:DNA Probes; D005819:Genetic Markers; D019914:Taq Polymerase