Oxysterols induce apoptosis and accumulation of cell cycle at G(2)/M phase in the human monocytic THP-1 cell line.
The cytotoxic activity of oxysterols, 7 beta-hydroxycholesterol (7 beta-OHC) and 25-hydroxycholesterol (25-OHC), has been evaluated using various leukemia cell lines. Among the tested cell lines, both oxysterols showed the highest cytotoxicity to THP-1, human monocytic leukemia cell line. These oxysterols induced apoptosis through down-regulation of Bcl-2 expression and activation of caspases. Also, the oxysterols showed the accumulation at G(2)/M phase of cell cycle through down-regulation of cyclin B1 expression. Taken together, these results indicated that both 7 beta-OHC and 25-OHC inhibited the proliferation of THP-1 cells through apoptosis and cell cycle accumulation at G(2)/M phase.
Lim
Hee-Kyoung
HK
Department of Biochemistry, College of Medicine Cheju National University, 1 Ara 1-dong, Jeju, Jeju-do, 690-756, South Korea.
Kang
Hee-Kyoung
HK
Yoo
Eun-Sook
ES
Kim
Bum-Joon
BJ
Kim
Youn-Woo
YW
Cho
Moonjae
M
Lee
Jae-Hwa
JH
Lee
Yun-Sil
YS
Chung
Myung-Hee
MH
Hyun
Jin-Won
JW
D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053938:DNA Fragmentation; D005434:Flow Cytometry; D016195:G2 Phase / Q000187:drug effects*; D018922:HL-60 Cells / Q000473:pathology; D006801:Humans; D006888:Hydroxycholesterols / Q000494:pharmacology*; D007938:Leukemia / Q000473:pathology; D007948:Leukemia, Monocytic, Acute / Q000473:pathology*; D015459:Leukemia-Lymphoma, Adult T-Cell; D008938:Mitosis / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D006888:Hydroxycholesterols; C011724:cholest-5-en-3 beta,7 alpha-diol; C007997:25-hydroxycholesterol