Local administration of growth hormone enhances periimplant bone reaction in an osteoporotic rabbit model.
OBJECTIVE
The purpose of this study was to determine whether rhGH administered locally during the surgical placement of an implant, in the tibia of an osteoporotic rabbit model, would induce qualitative and quantitative differences in peri-implant bone reaction.


METHODS
Eight New Zealand rabbits were ovariectomized and fed with a low-calcium diet (with 0.07% of calcium) to induce osteoporosis. After 6 weeks, an experimental titanium sheet was inserted into the rabbit tibiae. The rabbits were randomly divided into two groups. The experimental group was treated with 4 IU of rhGH added locally into the ostectomy as a lyophilized powder and the control group was left without any treatment. After 2 weeks, animals were sacrificed, tibial sections were prepared and bone-titanium interface was examined at light microscopy, using Masson, hematoxylin-eosin and Pichrosirius stains. Light microscopic morphometry and densitometry were used to comparatively quantitate bone reaction.


CONCLUSIONS
Local administration of rhGH during the surgical placement of titanium sheets on the tibiae of an osteoporotic rabbit model enhances periosteal and transcortical reaction and mineralization of osteoid 14 days later around titanium sheets, without increasing bone resorption.
Tresguerres
Isabel F
IF
Department of Oral Medicine and Bucofacial Surgery, School of Dentistry, Complutense University, Madrid, Spain. f3f@eresmas.com
Clemente
Celia
C
Donado
Manuel
M
Gómez-Pellico
Luis
L
Blanco
Luis
L
Alobera
Miguel Angel
MA
Tresguerres
Jesús A F
JA
D000287:Administration, Topical; D000818:Animals; D001391:Azo Compounds; D015519:Bone Density / Q000502:physiology; D001857:Bone Matrix / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects* / Q000503:physiopathology / Q000601:surgery; D002113:Calcification, Physiologic / Q000187:drug effects; D004396:Coloring Agents; D003720:Densitometry; D015921:Dental Implants; D004195:Disease Models, Animal; D005260:Female; D005456:Fluorescent Dyes; D013006:Growth Hormone / Q000008:administration & dosage / Q000627:therapeutic use*; D016348:Osseointegration; D010024:Osteoporosis / Q000503:physiopathology*; D010027:Osteotomy; D010052:Ovariectomy; D010521:Periosteum / Q000187:drug effects; D011817:Rabbits; D011897:Random Allocation; D011994:Recombinant Proteins; D013223:Statistics as Topic; D013499:Surface Properties; D013977:Tibia / Q000601:surgery; D014025:Titanium / Q000737:chemistry
D001391:Azo Compounds; D004396:Coloring Agents; D015921:Dental Implants; D005456:Fluorescent Dyes; D011994:Recombinant Proteins; C009798:C.I. direct red 80; D013006:Growth Hormone; D014025:Titanium