Activation of PAF-synthesizing enzymes in rat brain stem slices after LTP induction in the medial vestibular nuclei.
LysoPAF acetyltransferase (lysoPAF-AT) and PAF-synthesizing phosphocholinetransferase (PAF-PCT) are the two enzymes which catalyze the final reactions for the synthesis of PAF. Their activities, assayed in the homogenate of rat brain stem slices and under their optimal conditions, increased 5 min after high frequency stimulation of vestibular afferents, inducing LTP in the medial vestibular nuclei. The activity of phosphatidylcholine-synthesizing phosphocholinetransferase, was not affected. Sixty minutes from the induction of LTP, PAF-PCT activity, but not that of lysoPAF-AT, was still significantly higher with respect to 5 min test stimulated control. We used AP-5 to verify whether this increase was strictly dependent upon LTP induction, which requires NMDA receptor activation. In AP-5 treated slices, lysoPAF-acetyltransferase and PAF-synthesizing phosphocholinetransferase activities increased, but they were reduced after high frequency stimulation under AP-5. In conclusion, we have demonstrated that the activities of PAF-synthesizing enzymes are activated soon after the induction of LTP and that this effect is linked to the activation of NMDA-receptors. We suggest that the enzyme activation by AP-5, preventing LTP, might be due to glutamate enhancement but, in neurons showing LTP and under normal conditions, the activation of potentiation mechanisms is critical for the enhancement of enzyme activities.
Francescangeli
Ermelinda
E
Department of Internal Medicine, Section of Biochemistry and Human Physiology, University of Perugia, Italy.
Grassi
Silvarosa
S
Pettorossi
Vito E
VE
Goracci
Gianfrancesco
G
D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D001933:Brain Stem / Q000201:enzymology*; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism*; D004558:Electric Stimulation; D004789:Enzyme Activation; D066298:In Vitro Techniques; D017774:Long-Term Potentiation; D010972:Platelet Activating Factor / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D014726:Vestibular Nuclei / Q000502:physiology*
D010972:Platelet Activating Factor; D000123:Acetyltransferases; C046035:1-alkylglycerophosphocholine acetyltransferase; D002798:Diacylglycerol Cholinephosphotransferase