title: alpha 11beta 1 integrin recognizes the GFOGER sequence in interstitial collagens.
abstract: The integrins alpha(1)beta(1), alpha(2)beta(1), alpha(10)beta(1), and alpha(11)beta(1) are referred to as a collagen receptor subgroup of the integrin family. Recently, both alpha(1)beta(1) and alpha(2)beta(1) integrins have been shown to recognize triple-helical GFOGER (where single letter amino acid nomenclature is used, O = hydroxyproline) or GFOGER-like motifs found in collagens, despite their distinct binding specificity for various collagen subtypes. In the present study we have investigated the mechanism whereby the latest member in the integrin family, alpha(11)beta(1), recognizes collagens using C2C12 cells transfected with alpha(11) cDNA and the bacterially expressed recombinant alpha(11) I domain. The ligand binding properties of alpha(11)beta(1) were compared with those of alpha(2)beta(1). Mg(2+)-dependent alpha(11)beta(1) binding to type I collagen required micromolar Ca(2+) but was inhibited by 1 mm Ca(2+), whereas alpha(2)beta(1)-mediated binding was refractory to millimolar concentrations of Ca(2+). The bacterially expressed recombinant alpha(11) I domain preference for fibrillar collagens over collagens IV and VI was the same as the alpha(2) I domain. Despite the difference in Ca(2+) sensitivity, alpha(11)beta(1)-expressing cells and the alpha(11) I domain bound to helical GFOGER sequences in a manner similar to alpha(2)beta(1)-expressing cells and the alpha(2) I domain. Modeling of the alpha I domain-collagen peptide complexes could partially explain the observed preference of different I domains for certain GFOGER sequence variations. In summary, our data indicate that the GFOGER sequence in fibrillar collagens is a common recognition motif used by alpha(1)beta(1), alpha(2)beta(1), and also alpha(11)beta(1) integrins. Although alpha(10) and alpha(11) chains show the highest sequence identity, alpha(2) and alpha(11) are more similar with regard to collagen specificity. Future studies will reveal whether alpha(2)beta(1) and alpha(11)beta(1) integrins also show overlapping biological functions.
authors.affiliation: Department of Medical Biochemistry and Microbiology, Biomedical Center, Box 582, Uppsala University, Uppsala S-751 23, Sweden.
authors.forename: Wan-Ming
authors.initials: WM
authors.lastname: Zhang
authors.forename: Jarmo
authors.initials: J
authors.lastname: Kapyla
authors.forename: J Santeri
authors.initials: JS
authors.lastname: Puranen
authors.forename: C Graham
authors.initials: CG
authors.lastname: Knight
authors.forename: Carl-Fredrik
authors.initials: CF
authors.lastname: Tiger
authors.forename: Olli T
authors.initials: OT
authors.lastname: Pentikainen
authors.forename: Mark S
authors.initials: MS
authors.lastname: Johnson
authors.forename: Richard W
authors.initials: RW
authors.lastname: Farndale
authors.forename: Jyrki
authors.initials: J
authors.lastname: Heino
authors.forename: Donald
authors.initials: D
authors.lastname: Gullberg
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism*; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D016023:Integrins / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D051379:Mice; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry; D010649:Phenylalanine / Q000737:chemistry; D011233:Precipitin Tests; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D038981:Receptors, Collagen / Q000737:chemistry* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D016023:Integrins; D010455:Peptides; D038981:Receptors, Collagen; D011994:Recombinant Proteins; C467800:integrin alpha11beta1; D010649:Phenylalanine; D003094:Collagen; D008274:Magnesium; D002118:Calcium