title: A single-copy gene encodes Kex1, a serine endoprotease of Pneumocystis jiroveci.
abstract: We have cloned and characterized the kex1 gene of Pneumocystis jiroveci. Unlike the case for Pneumocystis carinii, in which the homologous PRT-1 genes are multicopy, kex1 is a single-copy gene encoding a protein homologous to fungal serine endoproteases, which localize to the Golgi apparatus. Thus, substantial biological differences can be seen among Pneumocystis species.
authors.affiliation: Critical Care Medicine Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Geetha
authors.initials: G
authors.lastname: Kutty
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Kovacs
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018628:Gene Dosage; D008969:Molecular Sequence Data; D043484:Proprotein Convertases; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013381:Subtilisins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; C470926:Kex1 protein, Pneumocystis jirovecii; D043484:Proprotein Convertases; D012697:Serine Endopeptidases; D013381:Subtilisins; C058751:KEX2 protein, S cerevisiae