[Amino acid sequence analysis of peptides that can react to the nuclear localization signals of human cytomegalovirus PPUL44].
OBJECTIVE
To find the peptides that have strong binding ability to the nuclear localization signals (NLS) of human Cytomegalovirus (HCMV) PPUL44 protein, and to analyze their amino acid sequences.


METHODS
Peptide clones that have binding ability to the NLSs were selected from a random peptide display library by using synthesized HCMV PPUL44 NLSA and NLSB respectively. The DNA sequences of these clones were detected by using "ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit". The amino acid sequences of the clones were analyzed for their homology, and the homologous sequences were compared with known sequences in protein bank.


RESULTS
The peptides that can bind to the HCMV PPUL44 NLSA(named as bNLSApep) have higher homologous amino acid sequence to importin alpha subunit, the bNLSApep48 sequence AVVTPVLTEILK is more similar to the sequence of the importin alpha subunit Arm 7 from amino acid 18 to 29, ANIFPVLTEILQ; The bNLSBpep 39 is similar to the sequence of all 8 Arm regions of importin alpha subunit from amino acid 10 to 21.


CONCLUSIONS
It is possible that the HCMV PPUL44 NLSA is a specific recognition site by importin alpha subunit, it can bind to the Arm 7 region of importin alpha subunit; HCMV PPUL44 NLSB is a non-specific recognition site for importin alpha subunit. This study provided an experimental data for the proposal that the Arm repeat region of importin alpha subunit is the binding region of NLS.
Ruan
Q
Q
Virus Laboratory, Second Affiliated Hospital of China Medical University, Shenyang 110003, China.
Ripalti
A
A
Landini
M P
MP
D000595:Amino Acid Sequence; D003587:Cytomegalovirus / Q000235:genetics*; D028884:Karyopherins / Q000378:metabolism; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals / Q000235:genetics*; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication
D028884:Karyopherins; D019913:Nuclear Localization Signals; D014764:Viral Proteins