Biological functions of the ISWI chromatin remodeling complex NURF.
The nucleosome remodeling factor (NURF) is one of several ISWI-containing protein complexes that catalyze ATP-dependent nucleosome sliding and facilitate transcription of chromatin in vitro. To establish the physiological requirements of NURF, and to distinguish NURF genetically from other ISWI-containing complexes, we isolated mutations in the gene encoding the large NURF subunit, nurf301. We confirm that NURF is required for transcription activation in vivo. In animals lacking NURF301, heat-shock transcription factor binding to and transcription of the hsp70 and hsp26 genes are impaired. Additionally, we show that NURF is required for homeotic gene expression. Consistent with this, nurf301 mutants recapitulate the phenotypes of Enhancer of bithorax, a positive regulator of the Bithorax-Complex previously localized to the same genetic interval. Finally, mutants in NURF subunits exhibit neoplastic transformation of larval blood cells that causes melanotic tumors to form.
Badenhorst
Paul
P
Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda Maryland 20892-4255, USA.
Voas
Matthew
M
Rebay
Ilaria
I
Wu
Carl
C
D000251:Adenosine Triphosphatases / Q000502:physiology*; D000818:Animals; D030801:Animals, Genetically Modified; D002843:Chromatin / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D005801:Genes, Homeobox / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D000076249:Heat Shock Transcription Factors; D006410:Hematopoiesis / Q000502:physiology*; D018398:Homeodomain Proteins; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D017600:Neuroectodermal Tumor, Melanotic / Q000235:genetics / Q000378:metabolism; D009707:Nucleosomes / Q000502:physiology; D012729:Sex Chromosome Aberrations; D014157:Transcription Factors / Q000502:physiology*; D015533:Transcriptional Activation / Q000187:drug effects / Q000502:physiology*; D014960:X Chromosome / Q000235:genetics
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C437989:E(bx) protein, Drosophila; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D018398:Homeodomain Proteins; C053134:Hsf protein, Drosophila; C086468:ISWI protein; D019476:Insect Proteins; D009707:Nucleosomes; D014157:Transcription Factors; C066177:Ubx protein, Drosophila; D000251:Adenosine Triphosphatases