[The biological characteristics of endothelial cells of human umbilical vein and the mechanism of intracellular signal transduction].
We observed the synthesis and release of vasoactive polypeptides (VPs) in the endothelial cells of human umbilical blood vessels (HUVEC). The results showed: (1) The content of VPs in the endothelial cells of non-innervated human umbilical vessels is higher than that of mesenteric vessels. (2) These VPs are synthesized by endothelial cells and released into the outside of cell bodies. (3) VIP and SP can stimulate the proliferation of HUVEC, but somatostatin (SOM) inhibits it. (4) VIP and SP increase opening probability of the Ca2+ channel in the membrane of HUVEC, while SOM decrease it, but VIP, SP and SOM result in the increase of concentrations of intracytosolic free Ca2+ and cAMP, while decreasing that of cGMP in HUVEC. In conclusion, we proved that there are local regulatory mechanisms in non-innervated human umbilical blood vessels, and VPs within endothelial cells may play an important role.
Yao
Z X
ZX
Department of Histology and Embryology, 3rd Military Medical University, Chongqing 400038.
D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004730:Endothelium, Vascular / Q000502:physiology*; D006801:Humans; D008642:Mesenteric Veins / Q000166:cytology; D015398:Signal Transduction; D013373:Substance P / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology*; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP; D002118:Calcium