The amyloid precursor-like protein 2 and the adenoviral E3/19K protein both bind to a conformational site on H-2Kd and regulate H-2Kd expression.
A protein of unknown physiological function, called amyloid precursor-like protein 2 (APLP2), forms an association with the murine class I molecule K(d) that is up-regulated by the presence of the adenoviral protein E3/19K. We have extended these findings to show that APLP2 and E3/19K associate preferentially with folded K(d) and not with the open form. APLP2 was detectable at the cell surface, but its surface expression was not up-regulated by the concurrent expression of K(d). Experimental down-regulation of APLP2 expression caused a consistent increase in the surface expression of K(d), indicating that APLP2 normally reduces K(d) surface expression. These data suggest a role for APLP2 in controlling the maturation of major histocompatibility complex class I molecules.
Morris
Chantey R
CR
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.
Petersen
Jason L
JL
Vargas
Shanna E
SE
Turnquist
Heth R
HR
McIlhaney
Mary M
MM
Sanderson
Sam D
SD
Bruder
Joseph T
JT
Yu
Yik Y L
YY
Burgert
Hans-Gerhard
HG
Solheim
Joyce C
JC
D017503:Adenovirus E3 Proteins / Q000737:chemistry / Q000378:metabolism*; D019943:Amino Acid Substitution; D016564:Amyloid beta-Protein Precursor / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000276:immunology; D006183:H-2 Antigens / Q000235:genetics* / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D007739:L Cells; D051379:Mice; D016297:Mutagenesis, Site-Directed; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding; D034741:RNA, Small Interfering / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
C489697:APLP1 protein, human; C084104:APLP2 protein, human; D017503:Adenovirus E3 Proteins; D016564:Amyloid beta-Protein Precursor; C078201:Aplp1 protein, mouse; C492952:Aplp2 protein, mouse; D017931:DNA Primers; D006183:H-2 Antigens; C024755:H-2K(K) antigen; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering