Enhanced expression of B7.2 (CD86) by percutaneous sensitization with house dust mite antigen.
House dust mite antigen is a well-known allergen in the pathogenesis of atopic dermatitis (AD), a chronic relapsing inflammatory skin disease. We evaluated the AD model mice sensitized with house dust mite antigen and observed a Th2-dominant immune response. In this experiment, BALB/c mice were sensitized percutaneously with house dust mite antigen three times with 7 days interval after skin barrier disruption. A remarkable infiltration of polymorphonuclear granulocytes and monocytes in the cutis was observed in mice treated with this antigen, high serum IgE levels and IL-4 mRNA expression in local lymph node cells was also observed. CD19(+) B cell numbers overturned to CD4(+) helper T cells. In these mice, there was significant increase of B7.2 (CD86) expression on CD19(+) B cells. These results indicate that house dust mite antigen sensitizes BALB/c mice and skews their Th1/Th2 balance toward Th2.
Santa
Kazuki
K
Oriental Medicine Research Center, The Kitasato Institute, Tokyo, Japan.
Watanabe
Kenji
K
Nakada
Tsutomu
T
Kato
Hiroyuki
H
Habu
Sonoko
S
Kubota
Shunichiro
S
D000279:Administration, Cutaneous; D000818:Animals; D000941:Antigens / Q000009:adverse effects*; D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics; D051940:B7-2 Antigen; D017449:Dermatitis, Allergic Contact / Q000209:etiology / Q000276:immunology*; D003876:Dermatitis, Atopic / Q000139:chemically induced* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood; D015847:Interleukin-4 / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D039981:Pyroglyphidae / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics; D018418:Th2 Cells / Q000276:immunology
D000941:Antigens; D015703:Antigens, CD; D051940:B7-2 Antigen; C497965:Cd86 protein, mouse; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D015847:Interleukin-4; D007073:Immunoglobulin E