title: NESbase version 1.0: a database of nuclear export signals.
abstract: Protein export from the nucleus is often mediated by a Leucine-rich Nuclear Export Signal (NES). NESbase is a database of experimentally validated Leucine-rich NESs curated from literature. These signals are not annotated in databases such as SWISS-PROT, PIR or PROSITE. Each NESbase entry contains information of whether NES was shown to be necessary and/or sufficient for export, and whether the export was shown to be mediated by the export receptor CRM1. The compiled information was used to make a sequence logo of the Leucine-rich NESs, displaying the conservation of amino acids within a window of 25 residues. Surprisingly, only 36% of the sequences used for the logo fit the widely accepted NES consensus L-x(2,3)-[LIVFM]-x(2,3)-L-x-[LI]. The database is available online at http://www.cbs.dtu.dk/databases/NESbase/.
authors.affiliation: Center for Biological Sequence Analysis, Building-208, Technical University of Denmark, DK-2800 Lyngby, Denmark.
authors.forename: Tanja
authors.initials: T
authors.lastname: la Cour
authors.forename: Ramneek
authors.initials: R
authors.lastname: Gupta
authors.forename: Kristoffer
authors.initials: K
authors.lastname: Rapacki
authors.forename: Karen
authors.initials: K
authors.lastname: Skriver
authors.forename: Flemming M
authors.initials: FM
authors.lastname: Poulsen
authors.forename: Søren
authors.initials: S
authors.lastname: Brunak
mesh_terms: D021581:Active Transport, Cell Nucleus; D020816:Amino Acid Motifs; D000818:Animals; D017124:Conserved Sequence; D030562:Databases, Protein; D007930:Leucine / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D011506:Proteins; D007930:Leucine