title: Rare earth-doped glass microbarcodes.
abstract: The development of ultraminiaturized identification tags has applications in fields ranging from advanced biotechnology to security. This paper describes micrometer-sized glass barcodes containing a pattern of different fluorescent materials that are easily identified by using a UV lamp and an optical microscope. A model DNA hybridization assay using these "microbarcodes" is described. Rare earth-doped glasses were chosen because of their narrow emission bands, high quantum efficiencies, noninterference with common fluorescent labels, and inertness to most organic and aqueous solvents. These properties and the large number (>1 million) of possible combinations of these microbarcodes make them attractive for use in multiplexed bioassays and general encoding.
authors.affiliation: Science and Technology Division, Corning Incorporated, Corning, NY 14831, USA. dejnekamj@corning.com
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Dejneka
authors.forename: Alexander
authors.initials: A
authors.lastname: Streltsov
authors.forename: Santona
authors.initials: S
authors.lastname: Pal
authors.forename: Anthony G
authors.initials: AG
authors.lastname: Frutos
authors.forename: Christy L
authors.initials: CL
authors.lastname: Powell
authors.forename: Kevin
authors.initials: K
authors.lastname: Yost
authors.forename: Po Ki
authors.initials: PK
authors.lastname: Yuen
authors.forename: Uwe
authors.initials: U
authors.lastname: Müller
authors.forename: Joydeep
authors.initials: J
authors.lastname: Lahiri
mesh_terms: D001709:Biotechnology; D004247:DNA / Q000235:genetics*; D005456:Fluorescent Dyes; D008674:Metals, Rare Earth; D009693:Nucleic Acid Hybridization / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D008674:Metals, Rare Earth; D004247:DNA