The Pap1-independent induction by metal ions of a third gene encoding glutathione S-transferase gene from the fission yeast.
A third gene that encodes glutathione S-transferase (GSTIII) was previously cloned from the fission yeast Schizosaccharomyces pombe. Using the GSTIII-lacZ fusion plasmid pGDA-19, its expression was shown to be enhanced by various metal ions. In the present study, four additional fusion plasmids, pGDA-29, pGDA-39, PGDA-49, and pGDA-59, were designed to carry 998, 378, 276, and 115 bp upstream regions from the translational initiation point, respectively. The major activation region was located between -998 and -378 bp upstream of the GSTIII gene. Regulatory sequences that are responsible for the induction by metal ions reside between -998 and -378 bp and between -276 and -115 bp upstream of the gene. The overexpressed Pap1 exerts a repression effect on the GSTIII expression via -998 to approximately -378 bp region, whereas it exerts an activation effect on the GSTIII expression via -270 to approximately -115 bp region. However, the induction of the GSTIII gene by metal ions occurs independent of Pap1.
Sa
Jae-Hoon
JH
Division of Life Sciences, Kangwon National University, Chuncheon 200-701, Korea.
Shin
Youn Hee
YH
Lim
Hye Won
HW
Kim
Kyunghoon
K
Park
Eun-Hee
EH
Lim
Chang-Jin
CJ
D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000494:pharmacology; D001252:Astringents / Q000494:pharmacology; D050976:Basic-Leucine Zipper Transcription Factors; D001665:Binding Sites; D019256:Cadmium Chloride / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004136:Dinitrobenzenes / Q000494:pharmacology; D005656:Fungal Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D000073718:Pancreatitis-Associated Proteins; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000201:enzymology; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D015854:Up-Regulation; D001616:beta-Galactosidase / Q000378:metabolism
D017607:Aluminum Compounds; D001252:Astringents; D050976:Basic-Leucine Zipper Transcription Factors; D002712:Chlorides; D017931:DNA Primers; D004268:DNA-Binding Proteins; D004136:Dinitrobenzenes; D005656:Fungal Proteins; D000073718:Pancreatitis-Associated Proteins; C105096:Pap1 protein, S pombe; C000612141:REG3A protein, human; D029702:Schizosaccharomyces pombe Proteins; C040234:1,2-dinitrobenzene; D000077410:Aluminum Chloride; D005982:Glutathione Transferase; D001616:beta-Galactosidase; D019256:Cadmium Chloride