Effect of the iron chelator deferoxamine on Trichomonas vaginalis in vitro.
The effect of 10 microMol, 15 microMol, 30 microMol, and 60 microMol concentrations of deferoxamine (DFO), a clinically approved iron chelator, was determined on viability and multiplication of Trichomonas vaginalis grown in TYM axenic culture medium at 24 hours interval. DFO killed all T. vaginalis isolates with a minimum lethal concentration of 30 microMol after 48 hours culture incubation with the drug. A potent and persistent inhibitory effect of DFO on the parasite viability and multiplication was recorded throughout the study till its end, in a drug concentration and time exposure-dependent manner. Furthermore, the present work studied the proteinase activity of T. vaginalis grown for 48 hours in DFO inoculated TYM medium, and recorded a significant decrease by all drug concentrations applied in the work. Different possible mechanisms of action of DFO against T. vaginalis and its possible use for treatment of trichomoniasis are discussed.
Mahmoud
Manal S E
MS
Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
D000818:Animals; D003470:Culture Media; D003676:Deferoxamine / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D010450:Endopeptidases / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007502:Iron Chelating Agents / Q000494:pharmacology* / Q000627:therapeutic use; D007700:Kinetics; D014245:Trichomonas Infections / Q000188:drug therapy; D014246:Trichomonas vaginalis / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development
D003470:Culture Media; D007502:Iron Chelating Agents; D010450:Endopeptidases; D003676:Deferoxamine