title: An improved method for recovering rabies virus from cloned cDNA.
abstract: A new system for recovery of rabies virus from cDNA plasmid, the transcription of which was driven by cellular RNA polymerase II, was developed. The plasmid contains full-length viral cDNA flanked by hammerhead ribozyme and hepatitis delta ribozyme sequences, arranged downstream of the cytomegalovirus (CMV) promotor. Transfection with the full-length cDNA plasmid together with helper plasmids encoding viral N, P, and L proteins without supply of T7 RNA polymerase produced a recombinant rabies virus in several cell lines. The efficiency of recovery between the conventional T7 promotor system and the new CMV promotor system was compared using these plasmid constructs. The newly established system is applicable to various cell lines and allows rapid and efficient generation of recombinant rabies virus.
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, 162-8640, Tokyo, Japan.
authors.forename: Ken ichi
authors.initials: Ki
authors.lastname: Inoue
authors.forename: Youko
authors.initials: Y
authors.lastname: Shoji
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
authors.forename: Toshio
authors.initials: T
authors.lastname: Iijima
authors.forename: Takeo
authors.initials: T
authors.lastname: Sakai
authors.forename: Kinjiro
authors.initials: K
authors.lastname: Morimoto
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D006224:Cricetinae; D003587:Cytomegalovirus; D018076:DNA, Complementary / Q000235:genetics*; D006801:Humans; D051379:Mice; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011820:Rabies virus / Q000235:genetics / Q000302:isolation & purification*; D011995:Recombination, Genetic; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D018076:DNA, Complementary