[An adenosine triphosphate bioluminescence assay for detecting the number of living cells].
The method for detecting the number of living cells was studied. Using an adenosine triphosphate (ATP) bioluminescence assay, the present authors reported a perfect linear relationship between lg ATP concentrations and lg luminescence counts (r = 0.9963) as well as a relationship between lg number of cells and lg ATP luminescence counts (r = 0.9922). The detectable cells ranged from 10(2) to 10(6) cells/ml, the coefficients of variation 1-3%. This method is simple, accurate and sensitive and has a high reproducibility.
Liu
S
S
Department of Obstetrics and Gynecology, Second Affiliated Hospital, WCUMS, Chengdu 610041.
Peng
Z
Z
Wang
H
H
Lou
J
J
He
B
B
Tang
Q
Q
Qiu
D
D
D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D002452:Cell Count / Q000379:methods; D005350:Fibroma / Q000473:pathology*; D008163:Luminescent Measurements; D051379:Mice; D014407:Tumor Cells, Cultured
D000255:Adenosine Triphosphate