title: In the spiny lobster (Panulirus interruptus) the prophenoloxidase is located in plasma not in haemocytes.
abstract: In the spiny lobster (Panulirus interruptus), unlike other crustaceans most of the prophenoloxidase (proPO) was detected in cell-free plasma (86.3%). In spite of its location, lobster proPO activating system has a similar activation mechanism to other crustacean proPO systems. Haemocyte lysate was able to activate the plasma proPO indicating location of the prophenoloxidase activating enzyme (PPAE) in haemocytes. Lobster haemocyte PPAE was isolated by affinity chromatography and its participation as activating enzyme was demonstrated. This enzyme is a serine-proteinase that transforms the inactive form (proPO) to an active one (phenoloxidase). The PPAE was also present in the cell-free supernatant of haemocytes previously incubated with Vibrio alginolyticus.
authors.affiliation: CIBNOR, P.O. Box 128, La Paz, BCS, 23000, Mexico
authors.forename: Jorge
authors.initials: J
authors.lastname: Hernández-López
authors.forename: Teresa
authors.initials: T
authors.lastname: Gollas-Galván
authors.forename: Silvia
authors.initials: S
authors.lastname: Gómez-Jiménez
authors.forename: Guillermo
authors.initials: G
authors.lastname: Portillo-Clark
authors.forename: Francisco
authors.initials: F
authors.lastname: Vargas-Albores
mesh_terms: D000818:Animals; D004156:Catechol Oxidase / Q000302:isolation & purification* / Q000378:metabolism; D002846:Chromatography, Affinity / Q000662:veterinary; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000302:isolation & purification* / Q000378:metabolism; D006434:Hemocytes / Q000201:enzymology*; D033521:Palinuridae / Q000166:cytology / Q000201:enzymology*; D012697:Serine Endopeptidases / Q000378:metabolism
chemical_list: D004792:Enzyme Precursors; C038451:pro-phenoloxidase; D004156:Catechol Oxidase; D012697:Serine Endopeptidases