[Effects of two haplotypes of HCAP1 gene on cellular gene expression levels in a human hepatocarcinoma cell line Hep3B].
OBJECTIVE
HCAP1 gene is a novel gene cloned in our laboratory, which has two haplotypes(N and M). This study was designed to investigate the effects of two haplotypes of HCAP1 gene on cellular gene expression levels in a human hepatocarcinoma cell line Hep3B.


METHODS
The Hep3B-Tet-on cells were transfected with HCAP1N or HCAP1M using liposome transfection reagent. In order to obtain the cells with high inducible expression of HCAP1N or HCAP1M, the transfected cells were screened by Western blot. Then, human atlas cDNA expression arrays were used to analyze the cellular gene expression profiles before and after HCAP1N or HCAP1M over-expression. The hybridization results were analyzed with FR-200 image system.


RESULTS
The profiles of differential gene expression before and after HCAP1N or HCAP1M over-expression were established. These data indicated that over-expression of HCAP1M resulted in an up-regulation of genes involved in cellular proliferation (e.g., c-erbB2 receptor and transcription factor DP2) and a down-regulation of genes involved in cell apoptosis (caspase9) or DNA excision repair (ERCC5). While overexpression of HCAP1N resulted in an up-regulation of genes involved in cellular suppressors (e.g., snoN and WAF1) or DNA excision repair (ERCC5) and a down-regulation of genes involved in anti-apoptosis (HSPs).


CONCLUSIONS
Over-expression of HCAP1M may promote cell proliferation. Over-expression of HCAP1N may suppress cell growth.
Wang
Jun-ru
JR
National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032, P. R. China.
Qin
Wen-xin
WX
He
Xiang-huo
XH
Pan
Yong
Y
Hu
Jian
J
Wan
Da-fang
DF
Gu
Jian-ren
JR
D000595:Amino Acid Sequence; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006239:Haplotypes; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism; D015778:Minor Histocompatibility Antigens; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D009857:Oncogenes; D020641:Polymorphism, Single Nucleotide; D017411:Ribonucleoproteins, Small Nuclear; D014162:Transfection
C406815:GEMIN4 protein, human; D015778:Minor Histocompatibility Antigens; D009687:Nuclear Proteins; D017411:Ribonucleoproteins, Small Nuclear