Double chimerism in recipient by transplantation of two allogeneic MHC-mismatched mouse fetal blood units.
We have constituted a mouse model for fetal blood transplantation (FBT) to cross over major histocompatibility complex (MHC) without causing serious GVHD. It seems that full matching at the MHC appears not necessary for FBT, while the nucleated cell dose is critical. Two fetal blood units were combined from different donors to increase the stem/progenitor cell dose so as to explore the possibility of MHC-mismatched allogeneic transplantation. 26 out of 40 mice in mixed FBT group survived in the observation period of 60 days after transplantation without obvious GVHD. Double chimerism was demonstrated by PCR and flow cytometric analysis; and skin transplantation test proved the induction of donor specific immune tolerance. Our data suggest that two MHC-mismatched allogeneic donor fetal blood units could simultaneously engraft and reconstitute immune and hematopoietic system in a mouse model. The result may be beneficial for the expansion of cord blood application and enables more patients to share the advantages of cord blood transplantation.
Shen
Bai-Jun
BJ
Department of Pediatrics, Qilu Hospital, Shandong University, Jinan 250012, China. shenbaijun@21cn.com
Liu
Xing-Xia
XX
Ju
Xiu-Li
XL
Zhang
Li-Ping
LP
Hou
Huai-Shui
HS
Ma
Xiu-Feng
XF
Shi
Qing
Q
D000818:Animals; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005312:Fetal Blood / Q000276:immunology / Q000637:transplantation*; D006086:Graft vs Host Disease / Q000276:immunology / Q000401:mortality; D006183:H-2 Antigens / Q000276:immunology*; D006410:Hematopoiesis / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D015996:Survival Rate; D018183:Transplantation Chimera / Q000235:genetics / Q000276:immunology*; D023001:Transplantation Tolerance / Q000276:immunology
D006183:H-2 Antigens; D004247:DNA