title: Pyruvate reverses metabolic effects produced by hypoxia in glioma and hepatoma cell cultures.
abstract: The intervention of pyruvate in glucose metabolism was investigated during hypoxic stress in tumour cell cultures having respiratory capacities under normoxic conditions. Results obtained with nuclear magnetic resonance (NMR) spectroscopy showed that, under normoxic conditions, rat glioma C6 and human hepatoma Hep G2 cell cultures metabolised [(13)C(1)]glucose into lactate, alanine, glutamate and other less abundant metabolites, as already known from the literature. In the absence of pyruvate, during hypoxia or cyanide poisoning, both cell types dramatically decreased the label into glutamate and accumulated [(13)C(3)]glycerol-3-phosphate. The compound was further identified by 31P NMR spectroscopy. The accumulation of the label in glycerol-3-phosphate, however, did not occur when the cells were incubated in the presence of pyruvate. The fate of the latter, followed under normoxic conditions by incubating cells with [(13)C(3)]pyruvate and natural glucose, showed that the label was mainly found in alanine, lactate and glutamate. Anoxic conditions increased the label in lactate and reduced that of glutamate. The data show a metabolic effect of pyruvate during mitochondrial blockade due to severe lack of oxygen in tumour cell lines.
authors.affiliation: Unité de biophysique, CRSSA, BP 87, 38702 La Tronche cedex, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Perrin
authors.forename: Emilie
authors.initials: E
authors.lastname: Roudier
authors.forename: Hervé
authors.initials: H
authors.lastname: Duborjal
authors.forename: Christine
authors.initials: C
authors.lastname: Bachelet
authors.forename: Catherine
authors.initials: C
authors.lastname: Riva-Lavieille
authors.forename: Xavier
authors.initials: X
authors.lastname: Leverve
authors.forename: Raphaël
authors.initials: R
authors.lastname: Massarelli
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology*; D015687:Cell Hypoxia / Q000187:drug effects*; D045744:Cell Line, Tumor; D005910:Glioma / Q000378:metabolism* / Q000473:pathology*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D000860:Hypoxia / Q000188:drug therapy; D019344:Lactic Acid / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D019289:Pyruvic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats
chemical_list: D019344:Lactic Acid; D019289:Pyruvic Acid; D005947:Glucose