[Determination of guanine plus cytosine content of Pseudomonas cocovenenans subsp farinofermantans with two methods and the comparative study].
The guanine plus cytosine content (G + C) mol% of Pseudomonas cocovenenans subsp farinofermantans, Burkholderia gladioli and Burkholderia cocovenenans, which were similar in biochemical characteristics, were determined with Tm values method and HPLC method, and the comparative study of these two methods was discussed. A negative conclusion could not be made from the results. The three bacteria were the same species. The HPLC method was more accurate than the Tm values method, but the repeatability of the latter was better.
Jiao
Z
Z
Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China.
Liu
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X
Yang
R
R
Guo
Z
Z
D015373:Bacterial Typing Techniques / Q000379:methods*; D019117:Burkholderia / Q000145:classification / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D003596:Cytosine / Q000032:analysis*; D004269:DNA, Bacterial / Q000032:analysis*; D006147:Guanine / Q000032:analysis*; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics; D013045:Species Specificity
D004269:DNA, Bacterial; D006147:Guanine; D003596:Cytosine