[Detection of TT virus DNA and sequence analysis of TTV gene in sera from young children in Nanjing].
OBJECTIVE
To investigate the prevalence of TTV genomes in young children from Nanjing and establish the PCR method for detecting TTV DNA.


METHODS
A hemi-nested polymerase chain reaction (PCR) assay was established to detect TTV DNA in 110 young children and two PCR-amplified products were sequenced and the data were analyzed with the computer programs.


RESULTS
TTV DNA was detected in 14 out of 110 children, and 222 bp sequences of 2 isolates from the children were analyzed. Compared with representative of published isolates, they have the nucleotide identity of 93.7%-98.6% with TX011.


CONCLUSIONS
The infection rate of TTV in children is 12.73%, and 2 isolates from children belong to subgroup G1b. Our results indicates that TTV could be transmitted via other pathway besides hematogenous one, and TTV could be carried by healthy population.
Pan
X
X
Academy of Life Science, Nanjing Normal University, Nanjing 210017, China.
Shan
X
X
Tang
J
J
D001483:Base Sequence; D002353:Carrier State; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D004266:DNA Virus Infections / Q000453:epidemiology / Q000821:virology*; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015995:Prevalence; D017422:Sequence Analysis, DNA; D022783:Torque teno virus / Q000235:genetics / Q000302:isolation & purification*
D004279:DNA, Viral