Immunoactivation of lectin-conjugated praecoxin A on IL-6, IL-12 expression.
Lectin-conjugated praecoxin A is a compound, which is combined Wheat Germ Agglutinin (WGA) Lectin with praecoxin A and also known to have an anti-tumor activity. In our lab, in order to investigate its immune reaction other than the anti-tumor activity ever known, we examined cytokines such as IL-6 and IL-12 through their mRNA expressions, which are generally secreted by macrophage both in vivo and in vitro. To analyze, we used RT-PCR for total RNAs of macrophages. As a result, we obtained that both in vitro and in vivo, lectin-conjugated praecoxin A showed an interesting increase on IL-6 and IL-12 even though it may be little hard to say the conjugated form is absolutely more effective than that of lectin or praecoxin A alone for immune response activities. Those results suggest that the conjugated form may give an additional opportunity in a future therapeutic use over its immuno activation properties.
Joo
Seong-Soo
SS
Division of Immunology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
Chang
Jae-Kwon
JK
Park
Jeong-Hwan
JH
Kang
Hee-Chul
HC
Lee
Do-Ik
DI
D000818:Animals; D002478:Cells, Cultured; D018664:Interleukin-12 / Q000096:biosynthesis* / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D008262:Macrophage Activation / Q000187:drug effects / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D037121:Plant Lectins / Q000276:immunology* / Q000378:metabolism* / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D013634:Tannins / Q000276:immunology* / Q000378:metabolism* / Q000494:pharmacology; D014909:Wheat Germ Agglutinins / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology
D015850:Interleukin-6; D037121:Plant Lectins; D012333:RNA, Messenger; D013634:Tannins; D014909:Wheat Germ Agglutinins; D018664:Interleukin-12