title: Influence of scaffold thickness and scaffold composition on bioartificial graft survival.
abstract: Biological scaffolds exhibit advantageous properties for tissue engineering of small diameter vessels. The influence of their extracellular matrix (ECM) components during in vivo repopulation is unknown. We implanted different xenogenic vascular matrices in a rat model to determine the influence of scaffold-thickness and ECM composition on in vivo repopulation. Decellularized ovine jugular vein (JV, n=42), carotid artery (CA, n=42) and aorta (AO, n=42) were implanted subcutaneously in the neck of adult male rats. Animals were sacrificed 2, 4 and 8 weeks after implantation. Cell and matrix morphology of explanted scaffolds were characterized by hematoxylin-eosin and pentachrome staining. Monoclonal anti-rat-CD31 was used to identify revascularization. Quantification of cell density was done by DNA-isolation. THICKNESS OF IMPLANTED XENOGENIC SCAFFOLDS VARIED ACCORDING TO THE MATERIAL USED (AO: 3.0-3.8mm; CA: 0.7-0.88mm; JV: 0.35-0.61mm). Immunohistology revealed complete repopulation of AO, CA, and JV scaffolds with endothelial cells and myofibroblasts within 2 weeks. After 8 weeks of implantation, AO scaffolds were completely covered by an endothelial monolayer and showed signs of a central matrix degeneration. JV scaffolds were completely degenerated at this stage. In contrast, CA scaffolds showed preserved ECM with a normal myofibroblast population and endothelial cell coverage.
authors.affiliation: Division of Thoracic and Cardiovascular Surgery, Hannover Medical School, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Walles
authors.forename: Tanja
authors.initials: T
authors.lastname: Herden
authors.forename: Axel
authors.initials: A
authors.lastname: Haverich
authors.forename: Heike
authors.initials: H
authors.lastname: Mertsching
mesh_terms: D000818:Animals; D001705:Bioprosthesis / Q000009:adverse effects*; D002339:Carotid Arteries / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism*; D006085:Graft Survival; D023421:Models, Animal; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*