Rapid enterovirus RNA detection in clinical specimens by using nucleic acid sequence-based amplification.
Enterovirus (EV) detection by nucleic acid sequence-based amplification was compared with EV isolation in cell culture. The NucliSens Basic kit (bioMerieux) was utilized for RNA detection. For virus isolation, samples were inoculated into MRC-5, primary rhesus monkey kidney, A549, rhabdomyosarcoma, and/or Buffalo green monkey kidney cells.
Landry
Marie L
ML
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA. marie.landry@yale.edu
Garner
Robin
R
Ferguson
David
D
D000293:Adolescent; D000328:Adult; D018929:Cell Culture Techniques; D002648:Child; D002675:Child, Preschool; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D012367:RNA, Viral / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D021161:Self-Sustained Sequence Replication / Q000379:methods*
D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic