[Isolation and biological activity of mycorrhizal fungi from Dendrobium candidum and D. nobile].
OBJECTIVE
To isolate the mycorrhizal fungi from the roots of wild Dendribium candidum and D. nobile collected from Yunnan and Sichuan provinces and determine their biological activities.


METHODS
The isolation was completed using solidified potato dextrose agar (PDA, Difco) of plates and the biological activities were tested by means of symbiotic germination of fungus-seedlings.


RESULTS
25 Species of mycorrhizal fungi were obtained and most of them belong to Basidiomycotina and Deuteromycotina. The preliminary biological activity test has shown that of these fungi 5 species help promote the seed germination of D. candidun, 7 species can establish the symbiotical relationship with seedlings of D. candium and D. nobile, and 3 species can stimulate the growth of seedlings of the two medicinal plants.


CONCLUSIONS
Isolation and screening of fungi helpful to the growth and development of Dendrobium is important in the production of the herb.
Guo
S X
SX
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100094, China.
Cao
W Q
WQ
Gao
W W
WW
D031667:Dendrobium / Q000254:growth & development / Q000382:microbiology*; D038821:Mycorrhizae / Q000302:isolation & purification* / Q000502:physiology; D018517:Plant Roots / Q000382:microbiology; D012639:Seeds / Q000254:growth & development; D013559:Symbiosis / Q000502:physiology