title: Galactosylated chitosan (GC)-graft-poly(vinyl pyrrolidone) (PVP) as hepatocyte-targeting DNA carrier. Preparation and physicochemical characterization of GC-graft-PVP/DNA complex (1).
abstract: Galactosylated chitosan was conjugated with poly(vinyl pyrrolidone) (PVP) as a hydrophilic group. The complex formation of GC-graft-PVP (GCPVP)/DNA complexes was confirmed by agarose gel electrophoresis. The morphology of the complex observed by atomic force microscopy had a compact and spherical shape, around 40 nm particle sizes at a charge ratio of 3. The binding strength of GCPVP 10K/DNA complex measured by ethidium bromide binding assay was superior to that of the GCPVP 50K/DNA one, probably attributable to its higher flexibility due to the smaller size, whereas the DNase I protection of GCPVP 10K/DNA complex was inferior to that of the GCPVP 50K/DNA one. This indicated that effective complex formation required both higher binding strength and minimal molecular weight of polycation enough to induce the condensation of DNA. The DNA-binding property of GCPVP mainly depended on the molecular weight of chitosan and composition of PVP.
authors.affiliation: School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, South Korea.
authors.forename: I K
authors.initials: IK
authors.lastname: Park
authors.forename: J E
authors.initials: JE
authors.lastname: Ihm
authors.forename: Y H
authors.initials: YH
authors.lastname: Park
authors.forename: Y J
authors.initials: YJ
authors.lastname: Choi
authors.forename: S I
authors.initials: SI
authors.lastname: Kim
authors.forename: W J
authors.initials: WJ
authors.lastname: Kim
authors.forename: T
authors.initials: T
authors.lastname: Akaike
authors.forename: C S
authors.initials: CS
authors.lastname: Cho
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002686:Chitin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry*; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D022781:Hepatocytes / Q000187:drug effects*; D008297:Male; D011205:Povidone / Q000008:administration & dosage / Q000737:chemistry*; D012474:Salmon
chemical_list: D004337:Drug Carriers; D002686:Chitin; D004247:DNA; D048271:Chitosan; D011205:Povidone