Specific expression of annexin III in rat-small-hepatocytes.
Small hepatocytes are cells that express characteristic phenotypes such as a high growth potential and differentiation capacity. In order to identify rat-small-hepatocyte specific proteins, we separated the cellular proteins of isolated small and parenchymal hepatocytes by 2D polyacrylamide gel electrophoresis. Comparison of their profiles revealed a protein with a molecular mass of 37 kDa in the small hepatocytes that was not present in the parenchymal hepatocytes. Proteolytic peptide mass fingerprinting was used to identify the protein and it was found to be annexin III. The validity of the identification was confirmed by Western blot analysis with anti-annexin III antibody.
Niimi
Shingo
S
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, 1-18-1 Kamiyoga Setagaya-ku, Tokyo 158-8501, Japan. niimi@nihs.go.jp
Oshizawa
Tadashi
T
Yamaguchi
Teruhide
T
Harashima
Mizuho
M
Seki
Taiichiro
T
Ariga
Toyohiko
T
Kawanishi
Toru
T
Hayakawa
Takao
T
D000818:Animals; D017318:Annexin A3 / Q000032:analysis* / Q000096:biosynthesis*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D022781:Hepatocytes / Q000737:chemistry* / Q000145:classification / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D010449:Peptide Mapping; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D017318:Annexin A3