title: Objective and noninvasive metabolic characterization of donor hearts by phosphorous-31 magnetic resonance spectroscopy.
abstract: OBJECTIVE
  The authors performed a multi-institutional, prospective, blind study on hearts from local donors to validate the feasibility and accuracy of the metabolic evaluation of human hearts by phosphorus-31 ( P) magnetic resonance spectroscopy (MRS) before transplantation.
  
  
  METHODS
  Twenty-one hearts were separated into two groups according to a transplantation score (TS) on the basis of the current clinical and echocardiographic evaluation as follows: TS1 (n=7), grafts for United Network for Organ Sharing (UNOS) 1 patients only; and TS2 (n=14), grafts suitable for UNOS 2 patients. All hearts were cold preserved with Celsior and underwent ex situ P MRS to measure ratios of various high-energy phosphate metabolites and the intracellular pH (pHi).
  
  
  RESULTS
  The total duration of the MRS procedure was 32 min, thereby not unacceptably increasing the total ischemic time for the transplanted grafts. Phosphocreatine (PCr) and inorganic phosphate (Pi ) was significantly different between the two groups (0.95+/-0.29 for TS1 and 2.05+/-0.74 for TS2). The difference in pHi was also significant (7.44+/-0.13 for TS1 and 7.64+/-0.19 for TS2).
  
  
  CONCLUSIONS
  Determination of PCr-Pi and pHi as markers of ischemic injury before transplantation can be considered as an objective and accurate criterion for the decision to accept or refuse heart grafts for transplantation.
authors.affiliation: Centre de Resonance Magnetique Biologique et Medicale, Faculte de Medecine, Marseille, France. frank.kober@medecine.univ-mrs.fr.
authors.forename: Frank
authors.initials: F
authors.lastname: Kober
authors.forename: Thierry
authors.initials: T
authors.lastname: Caus
authors.forename: Alberto
authors.initials: A
authors.lastname: Riberi
authors.forename: Sylviane
authors.initials: S
authors.lastname: Confort-Gouny
authors.forename: Yann
authors.initials: Y
authors.lastname: LeFur
authors.forename: Marguerite
authors.initials: M
authors.lastname: Izquierdo
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Ranjeva
authors.forename: Patrick
authors.initials: P
authors.lastname: Viout
authors.forename: Thierry
authors.initials: T
authors.lastname: Mesana
authors.forename: Dominique
authors.initials: D
authors.lastname: Metras
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Cozzone
authors.forename: Monique
authors.initials: M
authors.lastname: Bernard
mesh_terms: D000328:Adult; D005260:Female; D006321:Heart / Q000000981:diagnostic imaging*; D016027:Heart Transplantation; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008875:Middle Aged; D017682:Myocardial Stunning / Q000000981:diagnostic imaging; D009206:Myocardium / Q000378:metabolism*; D010759:Phosphorus Isotopes; D011446:Prospective Studies; D011877:Radionuclide Imaging; D014019:Tissue Donors
chemical_list: D010759:Phosphorus Isotopes