title: A Class II fructose-1,6-bisphosphate aldolase from a halophilic archaebacterium Haloferax mediterranei.
abstract: Fructose-1,6-bisphosphate (FBP) aldolase (EC 4.1.2.13) was purified 97-fold from a halophilic archaebacterium Haloferax mediterranei, with a specific activity of 2.8. The enzyme was characterized as a Class II aldolase on the basis of its inhibition by EDTA and other metal chelators. The enzyme had a specific requirement for divalent metal Fe(2+) for activity. Sulfhydryl compounds enhanced aldolase activity.
authors.affiliation: Radiation Biology and Biochemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.
authors.forename: Sandra E.
authors.initials: SE
authors.lastname: D'Souza
authors.forename: Wijaya
authors.initials: W
authors.lastname: Altekar