T-box binding site mediates the dorsal activation of myf-5 in Xenopus gastrula embryos.
Myf-5, a member of the muscle regulatory factor family of transcription factors, plays an important role in the determination, development, and differentiation of the skeletal muscle. Factors that regulate the expression of myf-5 itself are not well understood. We show here that a T-box binding site in the Xenopus myf-5 promoter mediated the activation of myf-5 expression through specific interaction with nuclear proteins of gastrula embryos. The T-box binding site could be bound by and respond to T-box proteins. T-box genes could induce Xmyf-5. The results suggest that T-box proteins are involved in the specification of myogenic mesoderm and muscle development.
Lin
Gu Fa
GF
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, People's Republic of China.
Geng
Xin
X
Chen
Ying
Y
Qu
Bin
B
Wang
Fubin
F
Hu
Ruiying
R
Ding
Xiaoyan
X
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002467:Cell Nucleus / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004268:DNA-Binding Proteins; D005775:Gastrula / Q000378:metabolism*; D017930:Genes, Reporter; D017403:In Situ Hybridization; D008648:Mesoderm / Q000166:cytology; D008957:Models, Genetic; D009124:Muscle Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D009132:Muscles / Q000166:cytology; D051787:Myogenic Regulatory Factor 5; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020825:T-Box Domain Proteins / Q000737:chemistry* / Q000378:metabolism; D015534:Trans-Activators; D014981:Xenopus; D029867:Xenopus Proteins
D004268:DNA-Binding Proteins; D009124:Muscle Proteins; C497419:Myf5 protein, Xenopus; D051787:Myogenic Regulatory Factor 5; D011500:Protein Synthesis Inhibitors; D020825:T-Box Domain Proteins; D015534:Trans-Activators; D029867:Xenopus Proteins; D003513:Cycloheximide; D004262:DNA Restriction Enzymes