SKY detection of chromosome rearrangements in two cases of tMDS with a complex karyotype.
In this study, we used spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH) as complementary techniques for the analysis of two therapy-related secondary myelodysplastic syndrome (t-MDS) cases with complex karyotypes, previously analyzed by G-banding. Different types of SKY's cytogenetic contributions include confirmation of G-banding results, identification of partially characterized rearrangements, identification of marker chromosomes unidentified by G-banding, and detection of cryptic reciprocal translocations. In particular, the ability of SKY to clarify a number of markers led to the comprehension of clonal evolution. The common aberration found in these two t-MDS cases was the fragility of chromosome 5 and monosomy of chromosome 18. We clearly present that the use of SKY combined with conventional G-banding analysis and FISH has assisted in the identification of important chromosomal events that may play a key role in the development of t-MDS.
Cohen
Ninette
N
Institute of Hematology and Pediatric Hemato-Oncology, The Chaim Sheba Medical Center, Tel-Hashomer, Israel. ninettac@yahoo.com
Trakhtenbrot
Luba
L
Yukla
Mona
M
Manor
Yosef
Y
Gaber
Elena
E
Yosef
Gabi
G
Amariglio
Ninette
N
Rechavi
Gideon
G
Amiel
Aliza
A
D000368:Aged; D002869:Chromosome Aberrations / Q000139:chemically induced / Q000528:radiation effects; D002877:Chromosomes, Human / Q000235:genetics; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D016609:Neoplasms, Second Primary / Q000235:genetics*; D032681:Spectral Karyotyping