title: DHA-enriched phospholipid diets modulate age-related alterations in rat hippocampus.
abstract: Our previous work on rat hippocampus showed that a loss of docosahexaenoic acid (DHA) occurs in the fatty acid composition of phosphatidylethanolamine (PE), plasmenylethanolamine (PmE) and phosphatidylserine (PS) with increasing age. The present study investigated whether a DHA-enriched phospholipid dietary supplement could restore DHA levels and cholinergic activity. Male rats were fed a balanced diet containing both linoleic and alpha-linolenic acids until the age of 2, 18 and 21 months. From 18 to 21 months, one subgroup received a diet supplemented with DHA-enriched phospholipids from egg yolk (E-PL), and another a diet with DHA-enriched phospholipids from pig brain (B-PL). Compared to the control diet, the E-PL diet restored the proportion of polyunsaturated fatty acids (PUFAs: 22:6n-3 and 20:4n-6) in PE and PmE, while enhancing spontaneous and evoked-acetylcholine (Ach) release. The B-PL diet had no effect on PUFAs, but increased basal extracellular levels of Ach in 21-month-old rats as compared to the age-matched control. Our results show that supplementation with DHA-enriched egg PL can enhance Ach release and correct PUFA composition.
authors.affiliation: EA 1223 G.E.M.C.I., Faculté de Médecine et Pharmacie, 86005 Poitiers, France. s.favreliere@voila.fr
authors.forename: S
authors.initials: S
authors.lastname: Favrelière
authors.forename: M C
authors.initials: MC
authors.lastname: Perault
authors.forename: F
authors.initials: F
authors.lastname: Huguet
authors.forename: D
authors.initials: D
authors.lastname: De Javel
authors.forename: N
authors.initials: N
authors.lastname: Bertrand
authors.forename: A
authors.initials: A
authors.lastname: Piriou
authors.forename: G
authors.initials: G
authors.lastname: Durand
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000375:Aging / Q000378:metabolism*; D000821:Animal Feed; D000818:Animals; D004032:Diet; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D004530:Egg Yolk; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D017551:Microdialysis; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism; D010743:Phospholipids / Q000494:pharmacology; D010955:Plasmalogens / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005231:Fatty Acids, Unsaturated; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D010743:Phospholipids; D010955:Plasmalogens; C020791:phosphatidal ethanolamines; D004281:Docosahexaenoic Acids; D000109:Acetylcholine; D011188:Potassium