Relationship between lipophilicity of C6-10 hydrocarbon solvents and their ROS-inducing potency in rat cerebellar granule cells.
We have studied the effects of aliphatic, alicyclic, and aromatic C6-10 solvents on the formation of reactive oxygen species (ROS) in rat cerebellar granule cell cultures. ROS formation was assessed by monitoring oxidation of 2',7'-dichlorofluorescin (DCFH) to the fluorescent compound 2',7'-dichlorofluorescein (DCF). We found that aromatic solvents with C > 7, and aliphatic and alicyclic solvents with C > or = 7 induce ROS formation in rat cerebellar granule cells in vitro. The response increased with increasing solvent concentration. The potency of the compounds within each homologous group seemed to be correlated to their octanol water partition-coefficients. The aromatic solvents were generally less efficient in inducing ROS formation than the aliphatic and the alicyclic compounds.
Dreiem
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VISTA, Norwegian Academy of Science and Letters/Statoil, Oslo, Norway. anne.dreiem@ffi.no
Myhre
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D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006841:Hydrocarbons, Aromatic / Q000494:pharmacology*; D050356:Lipid Metabolism; D008297:Male; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012997:Solvents / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D006841:Hydrocarbons, Aromatic; D017382:Reactive Oxygen Species; D012997:Solvents