title: Surface effects and desorption of tetracycline supramolecular complex on bovine dentine.
abstract: The aim of this in vitro study was to evaluate the antimicrobial activity, the substantivity, and surface effects of the inclusion compound tetracycline: beta-cyclodextrin on bovine roots. The antimicrobial activity was assessed by dentine slabs which had been immersed in the inclusion complex in concentrations 8.0%, 4.0%, 2.0%, 1.0%, 0.5% and 0.25% for 5min compared to a control of tetracycline hydrochloride. Each slab was tested in a broth of overnight culture of Actinobacillus actinomycetemcomitans (Y4-FDC). The inclusion complex significantly inhibited the A. actinomycetemcomitans (p<0.01) verified at concentrations from 1.0% to 8.0%. The substantivity of tetracycline was evaluated by the measure of desorption from the slabs previously immersed in solution samples and removed at 24h intervals. The tetracycline encapsulated in beta-cyclodextrin showed a flow rate near to zero order in comparison to free tetracycline. The surface morphology determined by SEM showed a more homogeneous and integrated layer with the complex compared to the effect of free tetracycline. We concluded that the root surfaces treated with tetracycline: beta-cyclodextrin release lower concentrations of active drug over 5 days at inhibitory concentrations against A. actinomycetemcomitans with enhanced disponibility in comparison to tetracycline.
authors.affiliation: Depto de Odontologia Restauradora, da Faculdade de Odontologia, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos 6627, CEP 31270-901, Belo Horizonte, Brazil.
authors.forename: A L
authors.initials: AL
authors.lastname: Pataro
authors.forename: C F
authors.initials: CF
authors.lastname: Franco
authors.forename: V R
authors.initials: VR
authors.lastname: Santos
authors.forename: M E
authors.initials: ME
authors.lastname: Cortés
authors.forename: R D
authors.initials: RD
authors.lastname: Sinisterra
mesh_terms: D000188:Actinobacillus / Q000187:drug effects; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D002417:Cattle; D003804:Dentin / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D008826:Microbial Sensitivity Tests; D013752:Tetracycline / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D013752:Tetracycline