Crystallization and preliminary crystallographic analysis of a partial extracellular fragment of a sperm membrane protein YWK-II/APPH related to the Alzheimer betaA4-amyloid precursor protein.
Crystals of a partial extracellular fragment of a human sperm membrane protein YWK-II/APPH have been grown at 291 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.8 A resolution at 100 K using Cu K(alpha) radiation. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 46.009, b = 67.387, c = 149.241 A, alpha = beta = gamma = 90 degrees. The presence of two molecules per asymmetric unit gives a crystal Volume per protein mass (V(M)) of 3.51 A(3) Da(-1) and a solvent content of 64.6% by Volume. A full set of X-ray diffraction data were collected to 2.8 A resolution from the native crystal.
Yang
Maojun
M
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, People's Republic of China.
Liu
Binbin
B
Zhang
Xiaodong
X
Zhou
Weihong
W
Gao
Feng
F
Pang
Hai
H
Miao
Shiying
S
Wang
Linfang
L
Rao
Zihe
Z
D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000235:genetics; D002462:Cell Membrane / Q000737:chemistry; D017124:Conserved Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments / Q000737:chemistry*; D013094:Spermatozoa / Q000737:chemistry*
C489697:APLP1 protein, human; C084104:APLP2 protein, human; D016564:Amyloid beta-Protein Precursor; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments