Lipofection strategy for the study of Xenopus retinal development.
The analysis of gene function during retinal development can be addressed by perturbing gene expression either by inhibition or by overexpression in desired regions and at defined stages of development. An in vivo lipofection strategy has been applied for stage-specific and region-specific expression of genes in Xenopus retina. Due to colipofection efficiency, this strategy enables us to study functional interaction of genes by lipofecting multiple expression constructs. This lipofection technique also allows us to transfect morpholino oligonucleotides into retinoblasts to block gene function. We present here various aspects of this technique, including recent improvements and modifications.
Ohnuma
Shin-ichi
S
The Hutchison/MRC Research Centre, Department of Oncology, University of Cambridge, Hills Road, UK. so218@cam.ac.uk
Mann
Fanny
F
Boy
Sébastien
S
Perron
Muriel
M
Harris
William A
WA
D000818:Animals; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental; D016376:Oligonucleotides, Antisense / Q000235:genetics; D012160:Retina / Q000254:growth & development* / Q000502:physiology*; D014162:Transfection / Q000295:instrumentation / Q000379:methods*; D014982:Xenopus laevis
D016376:Oligonucleotides, Antisense