[Immunophenotypic characteristics of multiple myeloma cells].
To investigate the immunophenotypic characteristics of multiple myeloma (MM) cells, 20 bone marrow samples from patients with multiple myeloma were analyzed by flow cytometry with three-color direct immunofluorescence staining. Results showed that all of myeloma cells expressed bright CD38, dim or negative CD45 and negative CD19. Most of the cells were CD56(+) and a small portions were ckappa(+) or clambda(+), or CD20(+). The phenotypes of normal plasmocyte, CD19(+) and CD56(-), except CD56(-) in one-third samples, were not appeared in all detected samples. It was concluded that the surface marker analysis of myeloma cells is a useful tool for diagnosis and further evaluating prognosis of multiple myeloma.
Li
Jin-Lan
JL
Institute of Hematology, Peking University, Beijing 100044, China.
Liu
Yan-Rong
YR
Chang
Yan
Y
Fu
Jia-Yu
JY
Chen
Shan-Shan
SS
D036541:ADP-ribosyl Cyclase / Q000276:immunology; D051997:ADP-ribosyl Cyclase 1; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000276:immunology; D018941:Antigens, CD19 / Q000276:immunology; D018951:Antigens, CD20 / Q000276:immunology; D001854:Bone Marrow Cells / Q000276:immunology*; D019002:CD56 Antigen / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000276:immunology; D008297:Male; D008562:Membrane Glycoproteins; D008875:Middle Aged; D009101:Multiple Myeloma / Q000276:immunology*
D015703:Antigens, CD; D018941:Antigens, CD19; D018951:Antigens, CD20; D019002:CD56 Antigen; D008562:Membrane Glycoproteins; D017493:Leukocyte Common Antigens; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1