Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP.
For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto, we describe a PCR-RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large-scale processing of field-collected larval and adult specimens to be identified in malaria vector studies.
Fanello
C
C
London School of Hygiene and Tropical Medicine, London, UK.
Santolamazza
F
F
della Torre
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A
D000349:Africa; D000375:Aging; D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D017344:Genes, Insect; D007814:Larva / Q000145:classification / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity