A cytogenetic footprint for mammary carcinomas induced by PhIP in rats.
2-Amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhIP), a mutagen/carcinogen belonging to the class of heterocyclic amines (HCAs) found in cooked meats, is a mammary gland carcinogen in rats and has been implicated in the etiology of certain human cancers including breast cancer. To gain insight into the genomic alterations associated with PhIP-induced mammary gland carcinogenesis, we used comparative genomic hybridization (CGH) to examine chromosomal abnormalities in rat mammary carcinomas induced by PhIP, and for comparison, by DMBA (7, 12-dimethylbenz[a]anthracene), a potent experimental mammary carcinogen. There was a consistent and characteristic pattern of chromosome-region loss in PhIP-induced carcinomas that clearly distinguished them from carcinomas induced by DMBA.
Christian
Allen T
AT
Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, P.O. Box 808, L-452, Livermore, CA 94551, USA. christian4@llnl.gov
D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D018983:DNA Footprinting; D005260:Female; D005504:Food Analysis; D007093:Imidazoles / Q000633:toxicity*; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced* / Q000235:genetics* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
D002273:Carcinogens; D007093:Imidazoles; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine