[Separation and cryopreservation of cord blood mononuclear cells].
The influencing factors on cord blood storage after collection and mononuclear cell separation as well as cryopreservation were studied. The mononuclear cell are separated from blood after blood collection, then cryopreserved and washed after thawed. Results showed that the cord blood kept at 4 degrees C or room temperature less than 24 hours after blood collection, mononuclear cell separated by hydroxyethylstarch and 2 centrifugations, mononuclear cell cryopreserved with 50% DMSO and autoplasma from cord blood as protectives and washing the cells after thawing. In conclusion, the optimal project in this study can effectively preserve cord blood mononuclear cells.
Lan
Jiong-Cai
JC
Department of Transfusion, Nanfang Hospital, The First Military Medical University, Guangzhou 510515, China.
Liu
Zhong
Z
Gan
Mao-Zhou
MZ
Chen
Qiang
Q
Zhang
Yin-Zhe
YZ
Meng
Qing-Bao
QB
D001793:Blood Preservation; D002469:Cell Separation / Q000379:methods*; D015925:Cryopreservation; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000502:physiology*