Ink-jet printer heads for ultra-small-drop protein crystallography.
Mass-produced automated piezoelectric driven picoliter delivery systems (printer heads) are fast, inexpensive, and reliable devices that are capable of delivering a very large range of volumes and are ideally suited for high-throughput protein crystallography studies. We used this technology to set up under-oil crystallization experiments with drop sizes from the 200-nL to 3-microL volume range, commonly used in protein crystallography, and show its application in setting ultra-small (2 nL) drops, the smallest drop volume reported to date for this type of assay.
Howard
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Institute de Genetique et de Biologie Moleculaire et Cellulaire, Illkirch, France. howard@iflysib.unlp.edu.ar
Cachau
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D000336:Aerosols; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000295:instrumentation*; D003902:Detergents; D004867:Equipment Design; D005990:Glycerol; D008899:Mineral Oil; D009113:Muramidase; D010316:Particle Size; D011136:Polysorbates; D011327:Printing / Q000295:instrumentation*; D011506:Proteins / Q000032:analysis*; D012996:Solutions
D000336:Aerosols; D003902:Detergents; D011136:Polysorbates; D011506:Proteins; D012996:Solutions; D008899:Mineral Oil; D009113:Muramidase; D005990:Glycerol