title: A genetic method to distinguish crossbred Inobuta from Japanese wild boars.
abstract: To distinguish pig-wild boar crossbred Inobuta from Japanese wild boar populations, a genetic method by using mitochondrial DNA (mtDNA) haplotypes and the nuclear glucosephosphate isomerase-processed pseudogene (GPIP) was developed. Sixteen mtDNA haplotypes from 152 wild boars from Kyushu, Shikoku and Honshu islands of Japan were distinct from those from Asian and European domestic pigs. Five alleles of GPIP were classified into two groups: 1). alleles GPIP*1, GPIP*3 and GPIP*3a from Japanese wild boars, Asian wild boars and domestic pigs; 2). alleles GPIP*4 and GPIP*4a from European wild boars and domestic pigs. An extensive genetic survey was done to distinguish the crossbred Inobuta from 60 wild boars hunted on Tsushima Island, Goto Islands, and Nagasaki and Ooita Prefectures. The mtDNA haplotypes from the 60 samples showed Japanese wild boars, but four wild boar samples from Nagasaki Prefecture had the European GPIP allele, GPIP*4. These results showed that nuclear DNA polymorphism analysis is useful, in addition to mtDNA haplotype assay, to detect "Inobuta" having the European genotype from Japanese wild boar populations.
authors.affiliation: Laboratory of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan. ishiguro@obihiro.ac.jp
authors.forename: Naotaka
authors.initials: N
authors.lastname: Ishiguro
authors.forename: Yuka
authors.initials: Y
authors.lastname: Naya
authors.forename: Motohiro
authors.initials: M
authors.lastname: Horiuchi
authors.forename: Morikazu
authors.initials: M
authors.lastname: Shinagawa
mesh_terms: D000483:Alleles; D000818:Animals; D000835:Animals, Wild / Q000235:genetics*; D001483:Base Sequence; D003433:Crosses, Genetic; D004272:DNA, Mitochondrial / Q000235:genetics*; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D007564:Japan; D011110:Polymorphism, Genetic / Q000235:genetics; D011544:Pseudogenes / Q000235:genetics*; D013045:Species Specificity; D013552:Swine / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D005956:Glucose-6-Phosphate Isomerase