title: Expression of proinflammatory cytokines and receptors by human fallopian tubes in organ culture following challenge with Neisseria gonorrhoeae.
abstract: Infection of the Fallopian tubes (FT) by Neisseria gonorrhoeae can lead to acute salpingitis, an inflammatory condition, which is a major cause of infertility. Challenge of explants of human FT with gonococci induced mRNA expression and protein secretion for the proinflammatory cytokines interleukin (IL)-1alpha, IL-1beta, and tumor necrosis factor alpha (TNF-alpha) but not for granulocyte-macrophage colony-stimulating factor. In contrast, FT expression of IL-6 and of the cytokine receptors IL-6R, TNF receptor I (TNF-RI), and TNF-RII was constitutive and was not increased by gonococcal challenge. These studies suggest that several proinflammatory cytokines are likely to contribute to the cell and tissue damage observed in gonococcal salpingitis.
authors.affiliation: Laboratorio de Inmunología de la Reproducción, Facultad de Química y Biología, Universidad de Santiago de Chile (USACH) Instituto Chileno de Medicina Reproductiva, Santiago, Chile. krmaisey@mi-mail.cl
authors.forename: Kevin
authors.initials: K
authors.lastname: Maisey
authors.forename: Gino
authors.initials: G
authors.lastname: Nardocci
authors.forename: Monica
authors.initials: M
authors.lastname: Imarai
authors.forename: Hugo
authors.initials: H
authors.lastname: Cardenas
authors.forename: Miguel
authors.initials: M
authors.lastname: Rios
authors.forename: Horacio B
authors.initials: HB
authors.lastname: Croxatto
authors.forename: John E
authors.initials: JE
authors.lastname: Heckels
authors.forename: Myron
authors.initials: M
authors.lastname: Christodoulides
authors.forename: Luis A
authors.initials: LA
authors.lastname: Velasquez
mesh_terms: D016207:Cytokines / Q000378:metabolism*; D005187:Fallopian Tubes / Q000276:immunology* / Q000382:microbiology*; D005260:Female; D006801:Humans; D007249:Inflammation; D007378:Interleukins / Q000378:metabolism; D018613:Microscopy, Confocal; D009344:Neisseria gonorrhoeae / Q000276:immunology* / Q000472:pathogenicity; D009924:Organ Culture Techniques; D018121:Receptors, Cytokine / Q000378:metabolism; D019947:Receptors, Interleukin-6 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D007378:Interleukins; D018121:Receptors, Cytokine; D019947:Receptors, Interleukin-6; D014409:Tumor Necrosis Factor-alpha