title: Formation of an F' plasmid by recombination between imperfectly repeated chromosomal Rep sequences: a closer look at an old friend (F'(128) pro lac).
abstract: Plasmid F'(128) was formed by an exchange between chromosomal Rep sequences that placed lac near dinB between many pairs of Rep sequences. Plasmid F'(128) is critical for selection-enhanced lac reversion (adaptive mutation), which requires prior lac amplification. The structure of F'(128) supports the idea that amplification is initiated by Rep-Rep recombination and that general mutagenesis requires coamplification of dinB (error-prone polymerase) with lac.
authors.affiliation: Department of Biology, University of Utah, Salt Lake City 84122, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Kofoid
authors.forename: Ulfar
authors.initials: U
authors.lastname: Bergthorsson
authors.forename: E Susan
authors.initials: ES
authors.lastname: Slechta
authors.forename: John R
authors.initials: JR
authors.lastname: Roth
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004265:DNA Helicases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005144:F Factor / Q000235:genetics*; D007763:Lac Operon; D008969:Molecular Sequence Data; D009154:Mutation; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; C506744:rep protein, E coli; D000251:Adenosine Triphosphatases; D004265:DNA Helicases