Simultaneous detection of the tumor suppressor FHIT gene and protein using the multi-functional biochip.
The tumor suppressor gene, fragile histidine triad (FHIT), encompasses the most common human chromosomal fragile site, at 3pl4.2. Detection of FHIT gene is important in cancer diagnostics since its alterations have been associated with several human cancers. A unique multi-functional biochip for simultaneous detection of FHIT DNA and FHIT protein on the same platform was applied. The design of the biochip is based on miniaturization of photodiodes, where functioning of multiple optical sensing elements, amplifiers, discriminators, and logic circuitry are integrated on a single IC board. Performance of biochip is based on biomolecular recognition processes using both DNA and protein bioreceptors, Cy5-labeled probes and laser excitation. Application of biochip for concurrent detection of various immobilized target DNA and protein molecules and multiplex of DNA and protein on the same microarray was accomplished. Linearity of biochip for quantitative measurements was demonstrated. Results demonstrated utility of this multi-functional biochip as a useful detection technology with applications in biological and clinical laboratories.
Askari
Minoo D F
MD
Advanced Monitoring Development Group, Life Sciences Division, Graduate School of Biomedical Sciences, Oak Ridge National Laboratory & University of Tennessee/Oak Ridge, Oak Ridge, TN 37831-6101, USA. askarimd@ornl.gov
Miller
Gordon H
GH
Vo-Dinh
Tuan
T
D017766:Acid Anhydride Hydrolases; D002893:Chromosomes, Human, Pair 3; D004273:DNA, Neoplasm / Q000032:analysis; D020869:Gene Expression Profiling; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics*; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D055095:Optics and Photonics; D013274:Stomach Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase