Monocyte chemoattractant protein 1 and chemokine receptor CCR2 productions in Guillain-Barré syndrome and experimental autoimmune neuritis.
Infiltration of activated lymphocytes and monocytes is a key phenomenon in the pathogenesis of Guillain-Barré syndrome (GBS) and experimental autoimmune neuritis (EAN). To investigate the role of chemokines, we determined the blood and nerve tissue expression of monocyte chemoattractant protein 1 (MCP-1), a major chemoattractant of monocytes and activated lymphocytes, and its receptor CCR2 in GBS and EAN. MCP-1 circulating levels (ng/ml) in GBS were increased at the time of progression, peaked at the time of plateau and normalized with recovery. MCP-1 circulating levels were the highest in the most disabled patients. The number of circulating CCR2 positive cells was lower in patients with GBS than in healthy subjects (p<0.004). In GBS, MCP-1 expression was observed in epineurial and endoneurial vessels, on infiltrating cells, Schwann cells and in the endoneurial extracellular matrix. Some CCR2 positive cells were observed in nerve biopsies of GBS patients. In EAN, a slight positivity for MCP-1 was observed in the sciatic nerve. There was no circulating CCR2 positive cells. However, at the time of plateau, a conspicuous infiltration of CCR2 positive cells was observed in the sciatic nerve that was no longer observed at the time of recovery. These results suggest that MCP-1 and CCR2 may participate to the recruitment of circulating mononuclear cells in nerve tissue in EAN and GBS.
Orlikowski
D
D
Réseau de Neuroimmunologie du Nerf Périphérique (AP/HP), INSERM E0011, Université Paris 12 Val-de-Marne, France.
Chazaud
B
B
Plonquet
A
A
Poron
F
F
Sharshar
T
T
Maison
P
P
Raphaël
J-C
JC
Gherardi
R K
RK
Créange
A
A
D000818:Animals; D002452:Cell Count; D018932:Chemokine CCL2 / Q000097:blood / Q000276:immunology*; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D005260:Female; D020275:Guillain-Barre Syndrome / Q000097:blood / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D009000:Monocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D009444:Neuritis, Autoimmune, Experimental / Q000097:blood / Q000276:immunology* / Q000473:pathology; D010525:Peripheral Nerves / Q000098:blood supply / Q000276:immunology* / Q000473:pathology; D010543:Peroneal Nerve / Q000098:blood supply / Q000276:immunology / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew; D054390:Receptors, CCR2; D019707:Receptors, Chemokine / Q000097:blood / Q000276:immunology*; D012584:Sciatic Nerve / Q000098:blood supply / Q000276:immunology / Q000473:pathology
C515334:CCR2 protein, human; C515336:Ccr2 protein, rat; D018932:Chemokine CCL2; D054390:Receptors, CCR2; D019707:Receptors, Chemokine