title: Genetic linkage maps of the West African clawed frog Xenopus tropicalis.
abstract: Amphibians, and particularly the African clawed frog Xenopus laevis, have been used for more than a century as models of vertebrate embryonic development. However, in many cases, elucidation of developmental functions of specific gene sequences could be severely impeded, because X. laevis is a tetraploid species, with multiple functional copies of many genes of interest. Recent studies have shifted focus to the West African or tropical clawed frog, X. tropicalis, the only known diploid species of the genus Xenopus. Here, we present two preliminary linkage maps, constructed by analysis of joint segregation of amplified fragment length polymorphism (AFLP) markers in a X. tropicalis interstrain hybrid. A total of 53 markers, including 51 AFLP markers and 2 isozyme markers, are presently assigned to 13 multipoint linkage groups on a map of the maternal strain, whereas 9 AFLP markers from the paternal strain are assigned to 3 linkage groups on a separate map. A dense genetic linkage map is essential in mapping new developmental mutants and determining their sequences by positional cloning.
authors.affiliation: Department of Carcinogenesis, University of Texas M.D. Anderson Cancer Center, Science Park--Research Division, Smithville, Texas 78957, USA.
authors.forename: Kelli J
authors.initials: KJ
authors.lastname: Kochan
authors.forename: David A
authors.initials: DA
authors.lastname: Wright
authors.forename: Lisa J
authors.initials: LJ
authors.lastname: Schroeder
authors.forename: Jianjun
authors.initials: J
authors.lastname: Shen
authors.forename: Donald C
authors.initials: DC
authors.lastname: Morizot
mesh_terms: D000349:Africa; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D008040:Genetic Linkage; D005819:Genetic Markers; D008957:Models, Genetic; D011110:Polymorphism, Genetic; D020033:Protein Isoforms; D014981:Xenopus / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D020033:Protein Isoforms