Preservation of human cell bioassay device for the on-site evaluation of environmental waters.
We have already developed a novel disposable bioassay device based on the low-density lipoprotein (LDL) uptaking activity of human hepatoblastoma Hep G2 cells in our previous work. However, this device is not readily applicable to evaluate river water toxicity on-site because it cannot be preserved for more than one week. In this work, we developed the method for preservation of the device to enable it to be preserved for at least one month. The device can be supplied to individual environmental sites without any facilities for cell culture. We can evaluate river water toxicity by 2 hours of exposure after thawing. Therefore, this kind of device could be a promising tool for daily water quality management.
Shoji
R
R
Department of Chemical Science and Engineering, Tokyo National College of Technology, 1220-2 Kunugida-Machi, Hachioji-shi, Tokyo 193-0997, Japan.
Sakai
Y
Y
Sakoda
A
A
Suzuki
M
M
D001681:Biological Assay / Q000295:instrumentation; D018929:Cell Culture Techniques; D008078:Cholesterol, LDL / Q000378:metabolism / Q000493:pharmacokinetics*; D003094:Collagen; D015925:Cryopreservation; D018197:Hepatoblastoma / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D016062:Porosity; D013048:Specimen Handling; D014407:Tumor Cells, Cultured; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D008078:Cholesterol, LDL; D014874:Water Pollutants, Chemical; D003094:Collagen