title: Topology models for 37 Saccharomyces cerevisiae membrane proteins based on C-terminal reporter fusions and predictions.
abstract: We provide experimentally based topology models for 37 integral membrane proteins from Saccharomyces cerevisiae. A C-terminal fusion to a dual Suc2/His4C topology reporter has been used to determine the location of the C terminus of each protein relative to the endoplasmic reticulum membrane, and this information is used in conjunction with theoretical topology prediction methods to arrive at a final topology model. We propose that this approach may be used to produce reliable topology models on a proteome-wide scale.
authors.affiliation: Department of Biochemistry and Biophysics, Stockholm University, Sweden.
authors.forename: Hyun
authors.initials: H
authors.lastname: Kim
authors.forename: Karin
authors.initials: K
authors.lastname: Melén
authors.forename: Gunnar
authors.initials: G
authors.lastname: von Heijne
mesh_terms: D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D020543:Proteome; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D020543:Proteome; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins