Application of improved iodine-azide procedure for the detection of thiouracils in blood serum and urine with planar chromatography.
The application of iodine-azide reaction for the determination of thiouracils in thin-layer chromatography and high-performance thin-layer chromatography is described. The developed plates were sprayed with a freshly prepared mixture of sodium azide, adjusted to a proper pH, and starch solution, and exposed to iodine vapour for 5 s. The detection limits were established at pmol level. The factors depending on the detection limits were described. A comparison of iodine-azide tests reaction with other procedures is presented. The developed method was applied to detection of thiouracils in blood serum and urine. The possibility of detection of a thiouracils mixture was demonstrated.
Zakrzewski
Robert
R
Department of Instrumental Analysis, University of Lódź, 163 Pomorska Street, 90-236, Lódź, Poland. robzak@chemul.uni.lodz.pl
Ciesielski
Witold
W
D001386:Azides / Q000737:chemistry*; D002855:Chromatography, Thin Layer / Q000379:methods*; D006801:Humans; D007455:Iodine / Q000737:chemistry*; D013889:Thiouracil / Q000032:analysis* / Q000097:blood / Q000652:urine
D001386:Azides; D013889:Thiouracil; D007455:Iodine