Modulation of murine macrophages and T lymphocytes by lysozyme dimer.
The effects of lysozyme dimer (2 and 20 microg/kg) administered i.p. once and four times to mice on the phagocytic and killing ability of peritoneal macrophages, interleukin-1 (IL-1) production by murine macrophages stimulated in vitro with lipopolisaccharide of E. coli and expression of thymocyte, splenocyte and mesenteric lymphonode cell CD3+, CD4+ and CD8+ markers were studied. It was found that lysozyme dimer administered once or four times at doses of 2 microg/kg and 20 microg/kg augments the phagocytic and killing activity of peritoneal macrophages. The strongest stimulating effect was noted after four injections of lysozyme dimer at a dose of 20 microg/kg. Moreover, lysozyme dimer is able to modulate the production of IL-1 by murine macrophages stimulated in vitro with LPS. Exposure to four doses of lysozyme dimer (20 microg/kg) enhances the synthesis and release of IL-1, but this drug administered once (2 microg/kg and 20 microg/kg) or four times (2 microg/kg) decreases IL-1 production by peritoneal macrophages. It was also found that administration of lysozyme dimer at a dose of 20 microg/kg, irrespective of the number of doses applied, increases the percentage of CD4+ thymocytes and splenocytes. Moreover, exposure to four doses of lysozyme dimer (2 and 20 microg/kg) increases the percentage of CD4+ and CD8+ mesenteric lymphonode cells.
Obmińska-Mrukowicz
B
B
Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Agricultural University, Norwida 31, 50-375 Wrocław, Poland.
Szczypka
M
M
Gaweda
B
B
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D007375:Interleukin-1 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009113:Muramidase / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects*
D007375:Interleukin-1; D009113:Muramidase