Stimulation of the secretion of pro-inflammatory cytokines by Bifidobacterium strains.
To characterize the ability of bifidobacteria to affect the production of macrophage-derived cytokines, a murine macrophage-like cell line, J774.1, was cultured in the presence of 27 strains of heat-inactivated bifidobacteria. Bifidobacterium adolescentis and B. longum, known as adult-type bifidobacteria, induced significantly more pro-inflammatory cytokine secretion, IL-12 and TNF-alpha, by J774.1 cells, than did the infant-type bifidobacteria, B. bifidum, B. breve, and B. infantis (P<0.01). In contrast, B. adolescentis did not stimulate the production of anti-inflammatory IL-10 from J774.1 cells as the other tested bacteria did. The results suggest that the adult-type bifidobacteria, especially B. adolescentis, may be more potent to amplify but less able to down-regulate the inflammatory response.
He
Fang
F
Technical Research Laboratory; Takanashi Milk Products Co., Ltd., Yokohama, Kanagawa 241-0023, Japan. he-fang@takanashi-milk.co.jp
Morita
Hirotsugu
H
Ouwehand
Arthur C
AC
Hosoda
Masataka
M
Hiramatsu
Masaru
M
Kurisaki
Jun-ichi
J
Isolauri
Erika
E
Benno
Yoshimi
Y
Salminen
Seppo
S
D000328:Adult; D000818:Animals; D001644:Bifidobacterium / Q000276:immunology*; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007249:Inflammation / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D013045:Species Specificity
D016207:Cytokines