Resonance double light scattering method for the determination of proteins with morin-CTMAB.
A new determination method of proteins with the limit of determination at nanogram levels is proposed by using a common spectrofluorimeter to detect intensity of resonance double line scattering (RDLS). Proteins including bovine serum albumin (BSA), human serum albumin (HSA) can combine with morin and cetyltrimethylammonium briomide (CTMAB) in the pH range 7.0-8.0 and produce enhanced RDLS signal at lambda(ex)/lambda(em) 305.0/610.0 nm. Optimization conditions for the morin-protein-CTMAB interaction were tested. In the studied system, BSA/CTMAB/morin = 1:2:3. The association constant of morin with BSA is 5.2 x 10(4). Under the optimum conditions, the linear range is 7.5 x 10(-8)-1.0 x 10(-5) g/ml for BSA, 2.5 x 10(-8)-5.0 x 10(-6) g/ml for HSA. The detection limits (S/N = 3) are 66.0 ng/ml for BSA and 23.0 ng/ml for HSA, respectively. Four synthetic samples were analyzed satisfactorily.
Liu
Rutao
R
Department of Chemistry, Shandong University, Jinan 250100, People's Republic of China.
Yang
Jinghe
J
Wu
Xia
X
Lan
Zengjun
Z
D000818:Animals; D002417:Cattle; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D006801:Humans; D008027:Light; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013053:Spectrophotometry; D047309:Flavones
D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D005419:Flavonoids; D012710:Serum Albumin, Bovine; C008548:morin; D047309:Flavones