title: Monoclonal antibody to shiga toxin 1, which blocks receptor binding and neutralizes cytotoxicity.
abstract: A monoclonal antibody, 5-5B, which neutralizes Shiga toxin 1 (Stx1) cytotoxicity of Escherichia coli, was constructed. An epitope analysis indicated that Asn55 in Stx1 B subunit was an important residue. This result and our previous results using an anti-Stx2 monoclonal antibody indicate that the region around the cysteine residue of the disulfide bond might be important for the neutralization of Stx cytotoxicity, making it a potential vaccination candidate.
authors.affiliation: Department of Infectious Diseases, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo 154-8567, Japan. hnakao@nch.go.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakao
authors.forename: Chiwa
authors.initials: C
authors.lastname: Kataoka
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Kiyokawa
authors.forename: Junichiro
authors.initials: J
authors.lastname: Fujimoto
authors.forename: Shinji
authors.initials: S
authors.lastname: Yamasaki
authors.forename: Tae
authors.initials: T
authors.lastname: Takeda
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002460:Cell Line; D000939:Epitopes; D004926:Escherichia coli / Q000378:metabolism / Q000472:pathogenicity*; D005434:Flow Cytometry; D006801:Humans; D016297:Mutagenesis, Site-Directed; D009500:Neutralization Tests; D011956:Receptors, Cell Surface / Q000378:metabolism; D022622:Shiga Toxin 1 / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D011956:Receptors, Cell Surface; D022622:Shiga Toxin 1