title: A novel syngeneic murine model for thoracic neuroblastoma obtained by intramediastinal injection of tumor cells.
abstract: In this study, a new syngeneic murine neuroblastoma (NB) model resembling stage 3 NB with thoracic localization has been set up by intramediastinal injection of tumor cells. Two NB cell lines, the murine Neuro2a, and the hybrid NXS2, have been injected in A/J mice. In vivo tumor growth and dissemination have been assessed by macroscopic and microscopic histological analysis performed at different times post injection. Tumor cell localization and growth patterns were compared to those obtained by i.v. and r.p. injections. The results indicated that in the thoracic model both tumor cell lines grow rapidly as huge tumor masses but do not metastatize to distant organs. This new model may be relevant for testing the efficacy of novel immunotherapeutic strategies for poor prognosis NB patients with localized disease.
authors.affiliation: Laboratory of Oncology, Istituto G. Gaslini, L go Gaslini 5, Genova 16148, Italy. mariavaleriacorrias@ospedale-gaslini.ge.it
authors.forename: Maria V
authors.initials: MV
authors.lastname: Corrias
authors.forename: Paola
authors.initials: P
authors.lastname: Bocca
authors.forename: Emanuela
authors.initials: E
authors.lastname: Anelli
authors.forename: Michele
authors.initials: M
authors.lastname: Cilli
authors.forename: Marzia
authors.initials: M
authors.lastname: Occhino
authors.forename: Vito
authors.initials: V
authors.lastname: Pistoia
authors.forename: Claudio
authors.initials: C
authors.lastname: Gambini
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000201:enzymology; D004195:Disease Models, Animal; D005260:Female; D007275:Injections, Intravenous; D008479:Mediastinal Neoplasms / Q000201:enzymology / Q000473:pathology*; D051379:Mice; D008805:Mice, Inbred A; D009447:Neuroblastoma / Q000201:enzymology / Q000556:secondary*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013899:Thoracic Neoplasms / Q000201:enzymology / Q000556:secondary*; D014407:Tumor Cells, Cultured; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D014446:Tyrosine 3-Monooxygenase