[Screening of short peptides that bind specifically to osteosarcoma cells by phage-displayed peptide library].
OBJECTIVE
To obtain short peptides which bind specifically to osteosarcoma cells os-732 by means of screening from 12 peptide libraries.


METHODS
Osteosarcoma cells os-732 were used as the target cells and osteoblasts as the absorber cells for subtraction biopanning from a 12-mer peptide phage-display library. After 3 rounds of screening, the positive phage clones were identified by cell enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, and the amino acid sequences were deduced by DNA sequencing.


RESULTS
Nine positive phage clones screened out of 20 clones showed specific binding with osteosarcoma os-732, but no conserved motif was found in these peptides.


CONCLUSIONS
The specific peptides screened from the phage library may be used as potential candidates as ligands for tumor-targeting therapy. The results also suggested that there are different epitopes on the surface of tumor cells.
Zhu
Guo-hong
GH
Department of Rachidial Diseases, Nanfang Hospital, China. ghzhu68@fimmu.edu.cn
Liu
Bei-yi
BY
Zheng
Shan-gen
SG
Lü
Hai
H
Shi
Zhan-jun
ZJ
D000595:Amino Acid Sequence; D001859:Bone Neoplasms / Q000378:metabolism*; D017124:Conserved Sequence; D006801:Humans; D007150:Immunohistochemistry; D012516:Osteosarcoma / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*
D019151:Peptide Library; D010455:Peptides