Composition of the water-soluble fraction of different cheeses.
Volatile and nonvolatile compounds present in the water-soluble fraction (WSF) and water-soluble fraction with molecular weight lower than 1000 Da (WSF < 1000 Da) of six Spanish cheeses, Cabrales, Idiazábal, Mahón, Manchego, Roncal, and a goat's milk cheese, were analyzed. Different nitrogen fractions (determined by Kjeldahl method), caseins (by capillary electrophoresis), peptides and amino acids (by HPLC), and volatile components (by dynamic headspace coupled to GC-MS) as well as mineral content in the cheese fractions were analyzed and compared. The different nitrogen and volatile compounds identified in the WSF were characteristic of each cheese variety. Cabrales cheese displayed the highest content of free amino acids and the highest quantity and variety of volatile compounds. The WSF < 1000 Da fraction was less representative, especially for volatile compounds, as some of the components were lost in the ultrafiltration. Alcohols were better recovered than ketones and esters.
Taborda
Gonzalo
G
Instituto de Fermentaciones Industriales (CSIC) and Instituto de Química Orgánica (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
Molina
Elena
E
Martínez-Castro
Isabel
I
Ramos
Mercedes
M
Amigo
Lourdes
L
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002364:Caseins / Q000032:analysis; D002417:Cattle; D002611:Cheese / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D010450:Endopeptidases / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006041:Goats; D008903:Minerals / Q000032:analysis; D009584:Nitrogen / Q000032:analysis; D009812:Odorants / Q000032:analysis; D010455:Peptides / Q000032:analysis; D012995:Solubility; D013030:Spain; D014835:Volatilization; D014867:Water
D000596:Amino Acids; D002364:Caseins; D008903:Minerals; D010455:Peptides; D014867:Water; D010450:Endopeptidases; D009584:Nitrogen