title: In vivo selection of Campylobacter isolates with high levels of fluoroquinolone resistance associated with gyrA mutations and the function of the CmeABC efflux pump.
abstract: Enrofloxacin treatment of chickens infected with fluoroquinolone(FQ)-sensitive Campylobacter promoted the emergence of FQ-resistant Campylobacter mutants which propagated in the intestinal tract and recolonized the chickens. The recovered isolates were highly resistant to quinolone antibiotics but remained susceptible to non-FQ antimicrobial agents. Specific single-point mutations in the gyrA gene and the function of the CmeABC efflux pump were linked to the acquired FQ resistance. These results reveal that Campylobacter is hypermutable in vivo under the selection pressure of FQ and highlight the need for the prudent use of FQ antibiotics.
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio 44691, USA.
authors.forename: Naidan
authors.initials: N
authors.lastname: Luo
authors.forename: Orhan
authors.initials: O
authors.lastname: Sahin
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.forename: Linda O
authors.initials: LO
authors.lastname: Michel
authors.forename: Qijing
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002169:Campylobacter Infections / Q000188:drug therapy; D016123:Campylobacter jejuni / Q000187:drug effects / Q000235:genetics*; D002645:Chickens; D002939:Ciprofloxacin / Q000494:pharmacology; D027081:DNA Gyrase / Q000187:drug effects / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D000077422:Enrofloxacin; D024841:Fluoroquinolones; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology; D015363:Quinolones / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; D009268:Nalidixic Acid; D000077422:Enrofloxacin; D002939:Ciprofloxacin; D027081:DNA Gyrase