title: Bipolar localization of putative photoreceptor protein for phototaxis in thermophilic cyanobacterium Synechococcus elongatus.
abstract: We identified an open reading frame from a database of the entire genome of Synechococcus elongatus, the product of which was very similar to pixJ1, which was proposed as photoreceptor gene for phototaxis in Synechocystis sp. PCC6803 [Yoshihara et al. (2000) Plant Cell Physiol. 41: 1299]. The mRNA of S. elongatus pixJ (SepixJ) was expressed in vivo as a part of the product of an operon. SePixJ was detected exclusively in the membrane fraction after cell fractionation. Immunogold labeling of SePixJ in ultra-thin sections indicated that it existed only in both ends of the rod-shaped cell; probably bound with the cytoplasmic membrane.
authors.affiliation: Graduate School of Integrated Science, Yokohama City University, Seto 22-2, Kanazawa-ku, Yokohama, 236-0027 Japan.
authors.forename: Youichi
authors.initials: Y
authors.lastname: Kondou
authors.forename: Norifumi
authors.initials: N
authors.lastname: Mogami
authors.forename: Fumiko
authors.initials: F
authors.lastname: Hoshi
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Kutsuna
authors.forename: Miki
authors.initials: M
authors.lastname: Nakazawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakurai
authors.forename: Minami
authors.initials: M
authors.lastname: Matsui
authors.forename: Takakazu
authors.initials: T
authors.lastname: Kaneko
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tabata
authors.forename: Ichiro
authors.initials: I
authors.lastname: Tanaka
authors.forename: Katsushi
authors.initials: K
authors.lastname: Manabe
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D000458:Cyanobacteria / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects; D015964:Gene Expression Regulation, Bacterial / Q000528:radiation effects; D008027:Light; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D020283:Photoreceptors, Microbial / Q000235:genetics* / Q000378:metabolism; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism / Q000528:radiation effects; D010834:Phytochrome / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000528:radiation effects; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D020283:Photoreceptors, Microbial; D045322:Photosynthetic Reaction Center Complex Proteins; D010834:Phytochrome