title: The association of class I HLA alleles and antibody levels after a single dose of measles vaccine.
abstract: Despite the success of the current measles vaccine in controlling disease in industrialized countries, the importance of vaccine failure has become increasingly apparent. Our objective was to determine if associations exist between seronegativity after measles vaccination and class I human leukocyte antigen (HLA) alleles. We undertook a cross-sectional observational study in Rochester, Minnesota, with 242 school-age children previously recruited from a communitywide seroprevalence study. We studied two groups of subjects: 72 were seronegative (EIA < or =0.8 after a single dose of measles vaccine) and 170 were seropositive (enzyme immunoassy [EIA] > or =1.0 after one dose). We used the resources of Mayo Clinic's tissue typing laboratory for serotyping class I HLA-A and HLA-B alleles via microlymphocytotoxicity assays. We found no statistically significant associations with class I HLA-A but did find associations with class I HLA-B, which includes alleles associated with seronegativity (B8, B13, and B44) and those associated with seropositivity (B7 and B51). Elucidation of the specific peptide-HLA complex interactions that lead to varying or failed immune responses may provide fertile groundwork for improved vaccines that can overcome limitations of the current live, attenuated measles vaccine.
authors.affiliation: Department of Pediatric and Adolescent MedicineMayo Clinic, Rochester, MN 55903, USA. jacobson.robert@mayo.edu
authors.forename: Robert M
authors.initials: RM
authors.lastname: Jacobson
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Poland
authors.forename: Robert A
authors.initials: RA
authors.lastname: Vierkant
authors.forename: V Shane
authors.initials: VS
authors.lastname: Pankratz
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Schaid
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Jacobsen
authors.forename: Jennifer St
authors.initials: JS
authors.lastname: Sauver
authors.forename: S Breanndan
authors.initials: SB
authors.lastname: Moore
mesh_terms: D000483:Alleles; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D003430:Cross-Sectional Studies; D006680:HLA Antigens / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008297:Male; D008458:Measles Vaccine / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D008458:Measles Vaccine