title: Gene expression profile analysis in human hepatocellular carcinoma by cDNA microarray.
abstract: We performed gene expression profiling of normal and hepatocellular carcinoma (HCC) liver tissues using a high-density microarray that contained 3,063 human cDNA. The results of a microarray hybridization experiment from eight different HCC tissues were analyzed and classified by the Cluster program. Among these differentially-expressed genes, the galectin-3, serine/threonine kinase SGK, translation factor eIF-4A, -4B, -3, fibroblast growth factor receptor, and ribosomal protein L35A were up-regulated; the mRNAs of Nip3, decorin, and the insulin-like growth factor binding protein-3 were down-regulated in HCC. The differential expression of these genes was further confirmed by an RT-PCR analysis. In addition, our data suggest that the gene expression profile of HCC varies according to the histological types.
authors.affiliation: Department of Immunology, School of Medicine, Kyungpook National University, Daegu 700-422, Korea.
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Chung
authors.forename: Young Kwan
authors.initials: YK
authors.lastname: Sung
authors.forename: Mohammad
authors.initials: M
authors.lastname: Farooq
authors.forename: Younghee
authors.initials: Y
authors.lastname: Kim
authors.forename: Sanguk
authors.initials: S
authors.lastname: Im
authors.forename: Won Young
authors.initials: WY
authors.lastname: Tak
authors.forename: Yoon Jin
authors.initials: YJ
authors.lastname: Hwang
authors.forename: Yang Il
authors.initials: YI
authors.lastname: Kim
authors.forename: Hyung Soo
authors.initials: HS
authors.lastname: Han
authors.forename: Jung-Chul
authors.initials: JC
authors.lastname: Kim
authors.forename: Moon Kyu
authors.initials: MK
authors.lastname: Kim
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism; D016000:Cluster Analysis; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation; D015870:Gene Expression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D012333:RNA, Messenger