title: Blue fluorescent antibodies as reporters of steric accessibility in virus conjugates.
abstract: Nonenveloped viruses provide the chemist with large, preassembled polyvalent protein scaffolds for modification. These structures are typically porous to small molecules but not to large ones. The solution-phase structures and reactivities of such assemblies may be substantially different than indicated by X-ray crystal structures. Here, the attachment of organic compounds to either the inside or outside surface of the cowpea mosaic virus (CPMV) coat protein was verified with an indicating antibody-antigen interaction. Antibody binding was subsequently blocked by the installation of poly(ethylene glycol) chains. These results typify the type of site-specific control that is available with CPMV and related virus building blocks.
authors.affiliation: Department of Chemistry, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Krishnaswami S
authors.initials: KS
authors.lastname: Raja
authors.forename: Kim D
authors.initials: KD
authors.lastname: Janda
authors.forename: Tianwei
authors.initials: T
authors.lastname: Lin
authors.forename: M G
authors.initials: MG
authors.lastname: Finn
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology; D000937:Antigen-Antibody Reactions; D036022:Capsid Proteins / Q000737:chemistry* / Q000276:immunology; D017798:Comovirus / Q000737:chemistry*; D005456:Fluorescent Dyes; D008958:Models, Molecular; D009930:Organic Chemicals / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D014780:Viruses / Q000737:chemistry
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011092:Polyethylene Glycols