[Study on the experimental infection of hepatitis G virus in rhesus monkey].
In order to study the experimental infection of HGV in rhesus monkey, the monkeys were infected using the plasma from a donor with HGV RNA positive. The second generation monkeys were infected with the sera of the first generation monkeys infected after 6 weeks, and also the third generation monkeys were infected with the sera of the second generation monkeys. HGV RNA were detected by RT-nPCR. The results showed that in sera of infected monkeys HGV RNA were positive after 1 week of inoculation, and were consecutively positive for up to 28 weeks at longest. The level of ALT raised a little in monkey No. 1 and higher than 100 U/L in No. 5. The liver biopsy showed viral hepatitis--like histological changes. Comparing the sequence of HGV 5'LTR from sera of the infected monkeys and the blood donor, the homogeneity, to strain HGU44402 was 98.33% and 95.83% to strain HGU36380 was 92.50% and 89.17%, respectively. The results suggested that the rhesus monkey is sensitive to HGV and is suitable for establishment of an animal model.
Mao
P
P
Department of Virology, 302 Hospital of PLA, Beijing 100039.
He
H
H
Hong
S
S
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D018178:Flaviviridae Infections; D029407:GB virus C; D006524:Hepatitis, Viral, Animal; D006801:Humans; D008253:Macaca mulatta; D008297:Male