title: Arginine methylation of recombinant murine fibrillarin by protein arginine methyltransferase.
abstract: Fibrillarin is a conserved nucleolar SnoRNP with a diverse N-terminal glycine- and arginine-rich (GAR) domain in most eukaryotes. This region in human fibrillarin is known to contain modified dimethylarginines. In this report we demonstrate that recombinant murine fibrillarin is a substrate for protein arginine methyltransferase, including the purified recombinant enzyme (rat PRMT1 and yeast RMT1) and the protein methyltransferases present in lymphoblastoid cell extracts. Our results of protease digestion, methylation competition reactions, and immunoblotting with a methylarginine-specific antibody all indicate that the methylation of fibrillarin is in the N-terminal GAR domain and arginyl residues are modified. Finally, amino acid analyses revealed that the modification of recombinant murine fibrillarin forms methylarginines, mostly as dimethylarginines.
authors.affiliation: Institute of Medicine, Chung Shan Medical and Dental University, Taichung, Taiwan, ROC.
authors.forename: Chia-Hui
authors.initials: CH
authors.lastname: Lin
authors.forename: Hung-Ming
authors.initials: HM
authors.lastname: Huang
authors.forename: Mingli
authors.initials: M
authors.lastname: Hsieh
authors.forename: K Michael
authors.initials: KM
authors.lastname: Pollard
authors.forename: Chuan
authors.initials: C
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008745:Methylation; D008780:Methyltransferases / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011484:Protein-Arginine N-Methyltransferases; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000737:chemistry / Q000235:genetics; D015003:Yeasts / Q000201:enzymology
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D047908:Intracellular Signaling Peptides and Proteins; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; C047458:fibrillarin; D001120:Arginine; D008780:Methyltransferases; C077013:PRMT2 protein, human; D011484:Protein-Arginine N-Methyltransferases; D010450:Endopeptidases