[Inhibition of tumor growth by a peptide fusion protein binding to vascular endothelial growth factor receptor Flt-1].
OBJECTIVE
Investigating the bio-activities of peptides selected from phage display peptide library with vascular endothelial growth factor receptor Flt-1.


METHODS
Activities of DHFR-F56/F90 binding to human ubilial vein endothelial cells were detected by immunocytochemistry, and the activity of antiangiogenesis was determined with chick embryo chorioallantoric membrane (CAM) assay. Balb/c nude mice were used as model to detect the activity of DHFR-F56/F90 on inhibiting tumor growth, and immunohistochemistry was employed to determine the localization of the DHFR-F56/F90 in tumor.


RESULTS
DHFR-F56/F90 can bind to HUVEC, and DHFR-F56 inhibite angiogenesis in CAM. Meanwhile DHFR-F56 can bind with tumor cells, induce tumor necrosis and inhibit tumor growth in vivo.


CONCLUSIONS
The peptide F56 is an effective antagonist of VEGF binding to Flt-1 and has a potent utility in antiangiogenesis and inhibiting tumor growth.
Lei
Hetian
H
Peking University School of Oncology, Beijing Institute for Cancer Research, Beijing 100034, China.
Shou
Chengchao
C
Wu
Jian
J
Liu
Xiaoying
X
He
Luowen
L
Liu
Meisheng
M
Guo
Qi
Q
Jiang
Beihai
B
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002642:Chick Embryo; D016228:Endothelial Growth Factors / Q000037:antagonists & inhibitors; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines / Q000037:antagonists & inhibitors; D051379:Mice; D008807:Mice, Inbred BALB C; D018919:Neovascularization, Physiologic / Q000187:drug effects; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology*; D013762:Tetrahydrofolate Dehydrogenase / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D042442:Vascular Endothelial Growth Factors
D000970:Antineoplastic Agents; D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D019151:Peptide Library; D010455:Peptides; D011993:Recombinant Fusion Proteins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D013762:Tetrahydrofolate Dehydrogenase; D040281:Vascular Endothelial Growth Factor Receptor-1