title: Promoter polymorphisms -359T/C and -303A/G of the catalytic subunit p110beta gene of human phosphatidylinositol 3-kinase are not associated with insulin secretion or insulin sensitivity in finnish subjects.
abstract: OBJECTIVE
  Phosphatidylinositol (PI) 3-kinase activity is required for insulin-stimulated translocation of GLUT4 transporters and glucose uptake and utilization. Therefore, genes encoding the subunits of PI 3-kinase are promising candidate genes for insulin resistance and type 2 diabetes. We recently cloned the catalytic subunit p110beta gene of human PI 3-kinase and reported two nucleotide polymorphisms, -359T/C and -303A/G, in the promoter region of this gene. In this study, we determined the effects of these polymorphisms on insulin secretion and insulin sensitivity.
  
  
  METHODS
  We studied two separate groups of Finnish nondiabetic subjects. Insulin secretion was evaluated by intravenous glucose tolerance test and insulin sensitivity by hyperinsulinemic-euglycemic clamp.
  
  
  RESULTS
  Our results showed that the -359T/C and -303A/G polymorphisms did not have a significant effect on fasting plasma insulin levels, insulin secretion, or insulin sensitivity.
  
  
  CONCLUSIONS
  It is unlikely that the promoter polymorphisms -359T/C and -303A/G of the catalytic subunit p110beta gene of human PI 3-kinase have a major impact on insulin secretion, insulin sensitivity, or the risk of type 2 diabetes in Finnish subjects.
authors.affiliation: A.I. Virtanen Institute for Molecular Sciences, Kuopio. Department of Medicine, University of Kuopio, Kuopio, Finland.
authors.forename: Maija
authors.initials: M
authors.lastname: Kossila
authors.forename: Jussi
authors.initials: J
authors.lastname: Pihlajamäki
authors.forename: Päivi
authors.initials: P
authors.lastname: Kärkkäinen
authors.forename: Raija
authors.initials: R
authors.lastname: Miettinen
authors.forename: Päivi
authors.initials: P
authors.lastname: Kekäläinen
authors.forename: Ilkka
authors.initials: I
authors.lastname: Vauhkonen
authors.forename: Seppo
authors.initials: S
authors.lastname: Ylä-Herttuala
authors.forename: Markku
authors.initials: M
authors.lastname: Laakso
mesh_terms: D000328:Adult; D015992:Body Mass Index; D020134:Catalytic Domain / Q000235:genetics; D005260:Female; D005387:Finland; D005838:Genotype; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007333:Insulin Resistance / Q000235:genetics*; D000078790:Insulin Secretion; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D007328:Insulin; D019869:Phosphatidylinositol 3-Kinases