5-Hydroxytryptamine and platelets: uptake and aggregation in hypoxic pulmonary hypertensive rats.
Pulmonary hypertension is associated with various alterations in 5-hydroxytryptamine (5-HT) physiology. In this study in platelets from hypoxic pulmonary hypertensive rats (10% O(2); 1 week) and normoxic rats (room air), (i) initial rates of specific [3H]5-HT uptake were measured and (ii) potentiation of collagen- and ADP-induced aggregation by 5-HT was quantified. The platelet count was almost halved in hypoxic rats. In uptake experiments, there was a decrease in 5-HT uptake in platelets from hypoxic compared with normoxic rats, due to a 36% reduction in the maximal initial rate of uptake. The aggregation experiments showed that 5-HT (1-100 microM) increased the magnitude of responses to collagen and the duration of responses to ADP, but there was no difference between hypoxic and normoxic rats. Abnormalities in platelet function may conceivably lead to increases in plasma 5-HT levels in hypoxic pulmonary hypertension, but are unlikely to aggravate pulmonary thromboembolism.
Awabdy
Doreen
D
Department of Physiology and Pharmacology, School of Biomedical Sciences, The University of Queensland, Queensland 4072, Brisbane, Australia.
Bryan-Lluka
Lesley J
LJ
Wanstall
Janet C
JC
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D003094:Collagen / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006400:Hematocrit; D006976:Hypertension, Pulmonary / Q000097:blood* / Q000209:etiology / Q000473:pathology; D000860:Hypoxia / Q000150:complications; D008297:Male; D009206:Myocardium / Q000473:pathology; D009929:Organ Size; D017374:Paroxetine / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D010976:Platelet Count; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000493:pharmacokinetics / Q000494:pharmacology*; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology
D000244:Adenosine Diphosphate; D003094:Collagen; D017374:Paroxetine; D012701:Serotonin; D017367:Selective Serotonin Reuptake Inhibitors