Distribution of apoptotic cells and apoptosis-related molecules in the developing murine palatine rugae.
Distribution of apoptotic cells and expression of the apoptosis-related factors p53, bcl-2 and bad during morphogenesis of the murine palatine rugae (PR) were examined histochemically using the terminal deoxynucleotidyl transferase-mediated UTP nick end-labeling (TUNEL) technique and specific antibodies against apoptosis and cell cycle-related molecules. Formation of the PR rudiment was controlled by cell proliferation and apoptosis in the palatal epithelium. TUNEL-positive cells were detected only at the epithelial placode area at 12.5-13.5 days post coitus (dpc), but only a few cells were positive at the protruding PR area at 14.5-16.5 dpc. Bcl-2 protein was expressed mainly in the areas outside of those containing TUNEL-positive cells at 15.5 -6.5 dpc. P53 protein was not detected throughout gestation. Bad was detected in the epithelial layer at 13.5 and 15.5 dpc and overlapping the apoptotic area at 13.5-15.5 dpc. Apoptosis of palatal epithelial cells might therefore involve spatiotemporally regulated expression of bad during murine PR development.
Amasaki
Hajime
H
Department of Veterinary Anatomy, Nippon Veterinary and Animal Science University, Musashino-shi, Tokyo, Japan.
Ogawa
Miyuki
M
Nagasao
Jun
J
Mutoh
Ken-ichiro
K
Ichihara
Nobutsune
N
Asari
Masao
M
D000818:Animals; D017209:Apoptosis; D002352:Carrier Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018507:Gene Expression Regulation, Developmental; D020287:In Situ Nick-End Labeling; D051379:Mice / Q000033:anatomy & histology / Q000196:embryology*; D021362:Palate, Hard / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000378:metabolism; D051025:bcl-Associated Death Protein
C494820:Bad protein, mouse; D002352:Carrier Proteins; C493961:Cdkn1b protein, mouse; D018797:Cell Cycle Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; D051025:bcl-Associated Death Protein; D050760:Cyclin-Dependent Kinase Inhibitor p27