[Sulindac induced apoptosis of HT-29 colon adenocarcinoma cell].
This study was intended to examine whether sulindac induces apoptosis in the HT-29 cells. Flow cytometry, transmission electron microscopy and DNA electrophoresis were used. Flow cytometry, transmission electron microscopy and DNA electrophoresis all demonstrated that sulindac could induce apoptosis of the HT-29 cell line in a time- and dose-dependent manner. After treatment with 0.3 mmol/L, 0.6 mmol/L and 1.2 mmol/L sulindac for 48 hours, apoptotic cells in the treatment groups reached 5.8%, 7.6% and 11.7% versus 2.9% in the control group; after treatment for 72 hours, apoptotic cells in the treatment groups increased to 12.5%, 15.4% and 24.4% versus 5.1% in the control group, respectively, P < 0.05. This study has demonstrated that colon adenocarcinoma HT-29 cell line can be induced by sulindac.
Yang
Z
Z
Department of Gastroenterology, First Affiliated Hospital, WCUMS, Chengdu 610041.
Ouyang
Q
Q
Wei
Y
Y
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D019073:HT29 Cells / Q000473:pathology*; D006801:Humans; D013467:Sulindac / Q000494:pharmacology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D013467:Sulindac