[Effects of musk glucoprotein on PAF production and cytosolic Ca2+ level in rat polymorphonuclear leukocytes in vitro].
OBJECTIVE
To investigate the effects of Musk glucoprotein on platelet activating factor (PAF) production and the concentration of cytosolic free Ca2+ in polymorphonuclear leukocytes of rat.


METHODS
An in vitro incubation system was used, production of PAF and activity of acetyl transferase were measured by isotope incorporation, the concentration of cytosolic free Ca2+ was quantitated using the fluorescent Ca2+ indicator Fura-2.


RESULTS
Musk-1 at concentration of 1-100 micrograms.ml-1 can significantly inhibit production of PAF, activity of acetyl transferase and the increase of cytosolic Ca2+ concentration in polymorphonuclear leukocytes of rat.


CONCLUSIONS
Part of mechanisms underlying antiinflammatory action of Musk-1 is through inhibiting the synthesis of PAF and the increase of cytosolic Ca2+ level.
Wang
W J
WJ
Institute of Materia Medica, Chinese Academy of Medical Sciences, Chinese Peking Union Medical College, Beijing 100050, China.
Zhou
L E
LE
Bai
J Y
JY
Cheng
G F
GF
Zhu
X Y
XY
D000123:Acetyltransferases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002469:Cell Separation; D005229:Fatty Acids, Monounsaturated; D005260:Female; D008297:Male; D008419:Materia Medica / Q000494:pharmacology*; D009504:Neutrophils / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D000511:alpha-Macroglobulins / Q000494:pharmacology*
D005229:Fatty Acids, Monounsaturated; D008419:Materia Medica; D010972:Platelet Activating Factor; D000511:alpha-Macroglobulins; C008563:musk; D000123:Acetyltransferases; D002118:Calcium