title: Uses and limitations of the XTT assay in studies of Candida growth and metabolism.
abstract: Colorimetric tetrazolium assays are used increasingly in studies of fungi, often in the absence of standardization or correlation with other methods. We examined species- and strain-related tetrazolium metabolism in Candida albicans and Candida parapsilosis by using XTT [2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide] and WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphonyl)-2H-tetrazolium] and found marked variations. Also, significant signal was often missed in the absence of dimethyl sulfoxide extraction.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Kuhn
authors.forename: M
authors.initials: M
authors.lastname: Balkis
authors.forename: J
authors.initials: J
authors.lastname: Chandra
authors.forename: P K
authors.initials: PK
authors.lastname: Mukherjee
authors.forename: M A
authors.initials: MA
authors.lastname: Ghannoum
mesh_terms: D002175:Candida / Q000254:growth & development* / Q000378:metabolism; D002455:Cell Division; D003124:Colorimetry / Q000379:methods; D006801:Humans; D013778:Tetrazolium Salts / Q000378:metabolism
chemical_list: D013778:Tetrazolium Salts; C042207:4-anisyltetrazolium blue