Overexpression of neurogenin1 induces neurite outgrowth in F11 neuroblastoma cells.
Neurogenin1 (Ngn1) is a basic helix-loop-helix (bHLH) transcription factor expressed in neuronal precursors in the developing nervous system. The function of Ngn1 in neurogenesis has been shown in various aspects. In this study, we investigated the neurogenic potential of Ngn1 using neuroblastoma cell line, F11, which could be induced to differentiate into neurons in the presence of cAMP. To investigate the expression of Ngn1, expression vectors for the full-length and the C- terminal deletion mutant of Ngn1 were constructed and their transactivation potential was verified using reporter gene containing the E-box sequence. Overexpression of the full-length Ngn1 induced neurite outgrowth in F11 cells in the absence of cAMP. A C-terminal deletion mutant, Ngn1(1-197), inhibited neurite outgrowth induced by cAMP in F11 cells. These results indicate that the Ngn1 plays an important role in differentiation of neuroblastoma cells and the C terminus of Ngn1 is essential for the efficient differentiation.
Kim
Soyeon
S
Department of Anatomy, School of Medicine, Ajou University, Suwon 442-749, Korea.
Ghil
Sung-Ho
SH
Kim
Sung-Soo
SS
Myeong
Hyeon-Ho
HH
Lee
Young-Don
YD
Suh-Kim
Haeyoung
H
D051792:Basic Helix-Loop-Helix Proteins; D015153:Blotting, Western; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D018257:Helix-Loop-Helix Motifs; D006801:Humans; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism*; D009447:Neuroblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015533:Transcriptional Activation
D051792:Basic Helix-Loop-Helix Proteins; D000242:Cyclic AMP; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D014157:Transcription Factors; C105389:NEUROG1 protein, human