title: Bacteriological and genetic assessment of game meat from Japanese wild boars.
abstract: Bacterial tests were used to assess bacterial contamination of game meat from Japanese wild boars. The bacterial contamination of wild boar meat was less than that of domestic pork, as determined by aerobic plate counts (APC) and coliform counts. None of the meat examined in this study was contaminated by Salmonella or E. coli O-157. To detect adulteration by domestic pig meat or European wild boar meat, 46 samples of game meat sold as Japanese wild boar were examined genetically. A total of 17 samples showed genetic haplotypes of European and Asian domestic pigs in the D-loop of mitochondrial DNA (mtDNA), and 16 samples showed nuclear glucosephosphate isomerase-processed pseudogene (GPIP) genotypes of European domestic pigs. The European GPIP genotypes of these samples were confirmed by PCR-RFLP analysis. These results indicate that some game meat sold as Japanese wild boar is adulterated by cross-breeding between pigs and wild boars or by contamination with meat from domestic pigs or European wild boars.
authors.affiliation: Laboratory of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Naya
authors.forename: Motohiro
authors.initials: M
authors.lastname: Horiuchi
authors.forename: Naotaka
authors.initials: N
authors.lastname: Ishiguro
authors.forename: Morikazu
authors.initials: M
authors.lastname: Shinagawa
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000235:genetics* / Q000382:microbiology*; D001947:Breeding; D015169:Colony Count, Microbial; D004272:DNA, Mitochondrial / Q000032:analysis; D005506:Food Contamination; D005516:Food Microbiology; D005838:Genotype; D006239:Haplotypes; D007564:Japan; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine / Q000235:genetics* / Q000382:microbiology*
chemical_list: D004272:DNA, Mitochondrial