Recombinant human interleukin-1alpha increases serum albumin, Gc-globulin, and alpha1-antitrypsin levels in burned mice.
The response to thermal injury is a complex physiologic process requiring communication between sites of injury and distant target organs. The liver, one of these target organs, synthesizes a family of secretory proteins, the acute phase proteins, that carries out specific immunoprotective functions. In this study we investigated the effects of daily recombinant human interleukin-1alpha (rhIL-1alpha) administration on the serum levels of negatively regulated, i.e., albumin and Gc-globulin and positively regulated, i.e., alpha1-antitrypsin, acute phase proteins in a murine model of thermal injury. Adult CF-1 female mice underwent a 6.5-seconds, 20% total burn surface area, full thickness steam injury, and received either intraperitoneal rhIL-1alpha (20 microg x kg(-1) x day(-1)) or diluent for 10 days. Seven and 14 days after injury, mice were sacrificed, and serum albumin, Gc-globulin and alpha1-antitrypsin levels were measured by crossed immunoelectrophoresis technique. Thermal injury significantly lowered serum albumin levels, tended to decrease Gc-globulin levels, and increased serum alpha1-antitrypsin levels. Daily rhIL-1alpha administration after burn injury prevented hypoalbuminemia, and increased serum levels of Gc-globulin and alpha1-antitrypsin. IL-1 therapy might be helpful to maintain the homeostasis and immunity of the host after thermal injury.
Koike
Kaoru
K
Department of Emergency and Critical Care Medicine, Graduate School of Medicine, Tohoku University, Sendai 980-8574, Japan. kkoike@gonryo.med.tohoku.ac.jp
Shinozawa
Yotaro
Y
Yamazaki
Motoyasu
M
Endo
Tomoyuki
T
Nomura
Ryosuke
R
Aiboshi
Junichi
J
Samarghandian
Saeed
S
Emmett
Merril
M
Peterson
Verlyn M
VM
D000818:Animals; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology; D012709:Serum Albumin / Q000096:biosynthesis*; D012867:Skin / Q000473:pathology; D014809:Vitamin D-Binding Protein / Q000096:biosynthesis*; D000515:alpha 1-Antitrypsin / Q000096:biosynthesis*
D007375:Interleukin-1; D011994:Recombinant Proteins; D012709:Serum Albumin; D014809:Vitamin D-Binding Protein; D000515:alpha 1-Antitrypsin