title: High-performance thin-layer chromatography-bioautography for multiple antibiotic residues in cow's milk.
abstract: An analytical method to identify and quantify multiple antibiotic residues (chloramphenicol, ampicillin, benzylpenicillin, dicloxacillin and erythromycin) in cow's milk by high-performance thin-layer chromatography (HPTLC) combined with bioautography was developed. The test microorganism used for bioautography was Bacillus subtilis ATCC 6633. Antibiotic residues were extracted with acetonitrile, fat eliminated with petroleum ether and residues isolated with dichloromethane The sensitivity of the method guarantees the detection of the above-mentioned antibiotics at levels below maximum residue limits (MRL) allowed for milk. Percentage recoveries ranged between 90 and 100%, with coefficients of variation between 7.2 and 21.3%. Some advantages of this methodology over thin-layer chromatography (TLC)/bioautography are also discussed.
authors.affiliation: Departamento de Producción Agricola y Animal, Universidad Autónoma Metropolitana, Unidad Xochimilco, Calzada del Hueso No. 1100, Col. Villa Quietud, 04960, D.F., Coyoacán, Mexico. acaram@cueyatl.uam.mx
authors.forename: Acacia
authors.initials: A
authors.lastname: Ramirez
authors.forename: Rey
authors.initials: R
authors.lastname: Gutiérrez
authors.forename: Gilberto
authors.initials: G
authors.lastname: Diaz
authors.forename: Clementina
authors.initials: C
authors.lastname: González
authors.forename: Norma
authors.initials: N
authors.lastname: Pérez
authors.forename: Salvador
authors.initials: S
authors.lastname: Vega
authors.forename: Mario
authors.initials: M
authors.lastname: Noa
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D002855:Chromatography, Thin Layer / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D008892:Milk / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents