Serial analysis of gene expression in circulating gamma delta T cell subsets defines distinct immunoregulatory phenotypes and unexpected gene expression profiles.
Gene expression profiles were compared in circulating bovine GD3.5+ (CD8-) and GD3.5- (predominantly CD8+) gammadelta T cells using serial analysis of gene expression (SAGE). Approximately 20,000 SAGE tags were generated from each library. A comparison of the two libraries demonstrated 297 and 173 tags representing genes with 5-fold differential expression in GD3.5+ and GD3.5- gammadelta T cells, respectively. Consistent with their localization into sites of inflammation, GD3.5+ gammadelta T cells appeared transcriptionally and translationally more active than GD3.5- gammadelta cells. GD3.5- gammadelta T cells demonstrated higher expression of the cell proliferation inhibitor BAP 37, which was associated with their less activated gene expression phenotype. The immune regulatory and apoptosis-inducing molecule, galectin-1, was identified as a highly abundant molecule and was higher in GD3.5+gammadelta T cells. Surface molecules attributed to myeloid cells, such as CD14, CD68, and scavenger receptor-1, were identified in both populations. Furthermore, expression of B lymphocyte-induced maturation protein, a master regulator of B cell and myeloid cell differentiation, was identified by SAGE analysis and was confirmed at the RNA level to be selectively expressed in gammadelta T cells vs alphabeta T cells. These results provide new insights into the inherent differences between circulating gammadelta T cell subsets.
Meissner
Nicole
N
Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717, USA.
Radke
Jay
J
Hedges
Jodi F
JF
White
Michael
M
Behnke
Michael
M
Bertolino
Shannon
S
Abrahamsen
Mitchell
M
Jutila
Mark A
MA
D000818:Animals; D016827:CD8 Antigens / Q000096:biosynthesis; D002417:Cattle; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology*; D015723:Gene Library; D016130:Immunophenotyping; D008213:Lymphocyte Activation / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D022423:Myeloid Cells / Q000276:immunology / Q000378:metabolism; D014176:Protein Biosynthesis / Q000276:immunology; D011499:Protein Processing, Post-Translational; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*; D014158:Transcription, Genetic / Q000276:immunology
D016827:CD8 Antigens; D008565:Membrane Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta