title: The transcription cycle in vivo. A blind watchmaker at work.
abstract: Recent work by the Cook and Misteli laboratories addresses the issue of assembly and action of the transcription machinery. Live cell studies of RNA polymerases I (RNAP1) and II (RNAP2) indicate that transcription complexes are assembled in a stochastic fashion from freely diffusible subunits. This contrasts to models involving stable holo-complexes.
authors.affiliation: Department of Cell Biology and Genetics, Erasmus MC Rotterdam, PO Box 1738, 3000 DR, Rotterdam, The Netherlands.
authors.forename: Wim
authors.initials: W
authors.lastname: Vermeulen
authors.forename: Adriaan B
authors.initials: AB
authors.lastname: Houtsmuller
mesh_terms: D004247:DNA / Q000235:genetics*; D007700:Kinetics; D008957:Models, Genetic; D010507:Periodicity; D012318:RNA Polymerase I / Q000235:genetics; D012319:RNA Polymerase II / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D004247:DNA; D012319:RNA Polymerase II; D012318:RNA Polymerase I