Profile of nonprotein thiols, lipid peroxidation and delta-aminolevulinate dehydratase activity in mouse kidney and liver in response to acute exposure to mercuric chloride and sodium selenite.
The effects of mercury (Hg(2+)) and selenite (Se(4+)) on delta-aminolevulinic acid dehydratase (delta-ALA-D) activity, 2-thiobarbituric acid reactive substances (TBARS) and nonprotein sulfhydryl content (NPSH) in mouse kidney and liver were investigated. Male mice were given a single i.p. injection of Hg(2+) and/or Se(4+) (25 micromol/kg) and were killed at 6, 12, 24 and 48 h after treatment. Hg(2+) inhibited renal delta-ALA-D at 6 and 12 h after treatment. Se(4+) abolished the inhibitory effect of mercury on renal delta-ALA-D at 12 h after treatment. Renal and hepatic NPSH content decreased after Hg(2+) exposure and selenite inhibited, at least in part, the Hg-induced oxidation of renal and hepatic NPSH. Se(4+) and Hg(2+), when injected alone, did not alter hepatic or renal TBARS levels; however, simultaneous exposure to these compounds increased hepatic and renal TBARS levels at 12 and 48 h after treatment, respectively. Present results suggest that selenium abolishes the interaction of Hg(2+) with sulfhydryl groups of protein and nonprotein sources.
Farina
Marcelo
M
Curso de Farmácia, Centro de Ciências da Saúde, Universidade Regional Integrada do Alto Uruguai e Missões, Campus de Erechim, RS, Brazil. marcelofarina@zipmail.com.br
Brandão
Ricardo
R
de Lara
Fabiana S
FS
Pagliosa
Letícia B
LB
Soares
Félix A
FA
Souza
Diogo O
DO
Rocha
João B T
JB
D000818:Animals; D007668:Kidney / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008627:Mercuric Chloride / Q000037:antagonists & inhibitors* / Q000494:pharmacology* / Q000633:toxicity; D051379:Mice; D000623:Porphobilinogen Synthase / Q000378:metabolism*; D018038:Sodium Selenite / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D008627:Mercuric Chloride; D000623:Porphobilinogen Synthase; D018038:Sodium Selenite