Use of two replacements of serum during bovine embryo culture in vitro.
This study evaluated the effect of two commercial serum replacements (Ultroser G and CPSR-3 on in vitro bovine embryo culture. In Experiment 1, zygotes were cultured in SOF+Ultroser G (2, 4 and 6%), SOF+CPSR-3 (2, 4 and 6%), and SOF+5% FCS (control). Blastocyst rates obtained after culturing with Ultroser G were lower than those with FCS. However, blastocyst rates for CPSR-3 were similar to those for serum. In addition, embryos produced in SOF+CPSR-3 had the same proportion inner cell mass number and total cell number as embryos cultured with FCS. In Experiment 2, a combination of serum replacements during different periods showed that treatment before the five-to eight-cell stages had no effect on further embryo development. However, treatments up to the morula stage affected blastocyst formation. The concentration of supplement and the timing of its inclusion in culture markedly affected embryo development. The serum replacement CPSR-3 can supplement embryo culture with blastocyst rates and quality similar to those for serum.
Duque
Paloma
P
Consejería de Medio Rural y Pesca, SERIDA-CENSYRA, Camino de los Claveles 604, Somió, 33203 Gijón, Asturias, Spain. pduque@serida.org
Gómez
Enrique
E
Díaz
Elena
E
Facal
Nieves
N
Hidalgo
Carlos
C
Díez
Carmen
C
D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002417:Cattle / Q000196:embryology*; D002452:Cell Count / Q000662:veterinary; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques / Q000662:veterinary; D004624:Embryo Transfer / Q000662:veterinary*; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D009028:Morula / Q000502:physiology; D015053:Zygote / Q000502:physiology*
D003470:Culture Media