[Extraction and isolation of active component for inhibiting PC3 cell proliferation in vitro from the fruit of Lycium barbarum L].
OBJECTIVE
To extract and isolate the active component inhibiting PC3 cell proliferation in vitro from the fruit of Lycium barbarum.


METHODS
The active components of the fruit of L. barbarum were extracted by water, 95% ethanol, 50% ethanol respectively. Then, ethanol extract was extracted with acetone and n-butanol and isolated by SiO2 column and purified by TLC to obtain the active component. The chemical structure of active component was identified by the spectral analysis. MTT assay was used to compare the inhibition activity(IC50) of PC3 cells proliferation of the all extracts.


RESULTS
According IC50, the most active component among all extracts isolated from the fruit of L. barbarum isscopoletin.


CONCLUSIONS
Scopoletin is the active component of the fruit of L. barbarum for inhibiting PC3 cell proliferation.
Liu
X L
XL
Institute of Materia Medica, Zhejiang Academy of Medical Sciences1, Hangzhou 310013, Zhejiang, China.
Sun
J Y
JY
Li
H Y
HY
Zhang
L
L
Qian
B C
BC
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D006801:Humans; D032305:Lycium / Q000737:chemistry*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000473:pathology; D012603:Scopoletin / Q000302:isolation & purification* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D012603:Scopoletin