title: 2,3-butanedione monoxime (BDM), a potent inhibitor of actin-myosin interaction, induces ion and fluid transport in MDCK monolayers.
abstract: Membrane-cytoskeleton interactions have been shown to be crucial to modulate polarity, cell shape and the paracellular pathway in epithelial MDCK cell monolayers. In particular, actin organization and myosin-dependent contractility play an important role in the regulation of these functions. Participation of myosin in vectorial transport, expressed as formation of domes, was investigated in confluent monolayers of high transepithelial electrical resistance (TER) plated on non-permeable supports. Cells exposed to 2,3-butanedione monoxime, a selective inhibitor of myosin ATPase, showed a remarkable increase in the number of domes. Replacement of extracellular Na+ and Cl- and inhibition of Na+-K+-ATPase blocked the induction of domes. The monoxime also caused a reduction of the TER leading to an increase in the paracellular flux of small molecular weight dextran. However, immunofluorescence microscopy of drug-treated cells showed that the localization and staining pattern of tight junction proteins ZO-1, occludin, and claudin 1, or the actin-myosin ring at the zonula adherens, were not modified. Treatment with the drug produced striking re-arrangements of actin filaments at the microvilli and at the basal level of the cells. Our data show that disruption of actin-myosin interaction at several cellular sites contributed importantly to the increased transport activity and the formation of the domes. These results point to the relevant role or actin-myosin dynamics and actin organization in the regulation of ion and water channel activity in these cells.
authors.affiliation: Department of Biologia Celular, Centro de Investigación y de Estudios Avanzados del IPN, Apartado 14-740, México, DF 07000, México.
authors.forename: Aida M
authors.initials: AM
authors.lastname: Castillo
authors.forename: José Luis
authors.initials: JL
authors.lastname: Reyes
authors.forename: Elsa
authors.initials: E
authors.lastname: Sánchez
authors.forename: Ricardo
authors.initials: R
authors.lastname: Mondragón
authors.forename: Isaura
authors.initials: I
authors.lastname: Meza
mesh_terms: D000199:Actins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D022005:Adherens Junctions / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002449:Cell Aggregation / Q000187:drug effects / Q000502:physiology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D002713:Chlorine / Q000172:deficiency; D003931:Diacetyl / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004285:Dogs; D017097:Electric Impedance; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D009218:Myosins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012964:Sodium / Q000172:deficiency; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism; D019108:Tight Junctions / Q000187:drug effects / Q000378:metabolism; D014882:Water-Electrolyte Balance / Q000187:drug effects / Q000502:physiology*
chemical_list: D000199:Actins; D004791:Enzyme Inhibitors; D007473:Ion Channels; C004717:diacetylmonoxime; D002713:Chlorine; D012964:Sodium; D009218:Myosins; D000254:Sodium-Potassium-Exchanging ATPase; D003931:Diacetyl