title: Evaluating the in vitro adhesive strength of biomaterials. Biosimulator for selective leak closure.
abstract: OBJECTIVE
  Surgical measures always involve problems of hemostasis and tissue management. Tissue sealing can be carried out in this case, with knowledge of the adhesive strength of liquid and fleece-bound sealing being of great clinical relevance. By employing a leak closure model, it has been possible to perform in vitro standardized technological-biomechanical experiments on a biomembrane mounted in a pressure chamber (biosimulator).
  
  
  RESULTS
  Liquid sealing (fibrin gluing: 4.1 hPa, photopolymerisate: 82.9 hPa) offers minimal and maximal adhesive strengths. Fleece-bound sealing does not depend on the carrier material and is always more stabile than pure liquid sealing, whereby systems that must be coated "on the spot" (prepare-to-use: 22.3-25.3 hPa) exhibit significantly lower adhesive strength (p < 0.0001) than a biodegradable collagen system, which is ready-to-use thanks to its fibrinogen-based coating (TachoComb H). Practicability, effectiveness and efficiency are further advantages of ready-to-use systems.
  
  
  CONCLUSIONS
  The biosimulator, presented here, is flexible in terms of its application for tissue management and it enables reproducible, economic and ecological evaluation of repair systems, e.g. tissue sealings.
authors.affiliation: Department of Paediatric Surgery, Friedrich-Alexander-University Erlangen-Nuremberg, Maximiliansplatz 2, 91054 Erlangen, Germany. roman.carbon@chir.imed.uni-erlangen.de
authors.forename: Roman T
authors.initials: RT
authors.lastname: Carbon
authors.forename: Sibylle
authors.initials: S
authors.lastname: Baar
authors.forename: Stefanie
authors.initials: S
authors.lastname: Kriegelstein
authors.forename: Hans P
authors.initials: HP
authors.lastname: Huemmer
authors.forename: Knut
authors.initials: K
authors.lastname: Baar
authors.forename: Stefanie-Isabell
authors.initials: SI
authors.lastname: Simon
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D003094:Collagen; D015718:Fibrin Tissue Adhesive; D006488:Hemostasis, Surgical; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing / Q000295:instrumentation*; D008954:Models, Biological; D010994:Pleura; D011312:Pressure; D034421:Sus scrofa; D014014:Tissue Adhesives
chemical_list: D001672:Biocompatible Materials; D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives; D003094:Collagen