[Plant regeneration from Agobacterium-mediated CTV-cp gene transformation of Poncirus trifoliata Raf].
OBJECTIVE
To lay a foundation for the resistant breeding, the anti-virus CTV-cp gene was transformed into the epicotyles mediated by Agrobacterium tumefaciens in Poncirus trifoliata.


METHODS
The explants used for the genetic transformation were the epicotyls from P. trifoliata. The Agrobacterum tumefaciens strain was EHA101 containing vector plasmid pGA482GG. The coat protein gene (CTV-cp gene), GUS gene and NPT II gene were introduced into the transformation plasmid.


RESULTS
Ceftaxime used as antibiotics was better than carbenicillin. The concentration was 300 mg.L-1; The selection pressure for kanamycin was 50 mg.L-1; 70.0% of the resistant plants were GUS-positive; extra gene was proved to be in P. trifoliata plant by southern blot examination.


CONCLUSIONS
An effective genetic transformation mediated by Agrobacterium tumefacines, which harbours a CTV-cp gene, has been developed in P. trifoliata. Transgenic CTV-cp plants were obtained.
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Institute of Chinese Materia Medica, University of Traditional Chinese Medical and Pharmaceutical Sciences, Guangzhou 510405, Guangdong, China.
Han
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ML
Li
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GG
D002213:Capsid; D036022:Capsid Proteins; D010942:Plant Viruses / Q000235:genetics*; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal / Q000235:genetics*; D032104:Poncirus / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics; D014170:Transformation, Genetic
D036022:Capsid Proteins; C068839:coat protein, citrus tristeza virus