[Functional phage display of human interferon alpha lc/86D].
We have successfully displayed human interferon-alpha lc/86D(IFN-alpha lc/86D) on the surface of the filamentous bacteriophage using a phagemid vector system (pCANTAB5E). The IFN-alpha lc/86D cDNA was fused to a DNA sequence encoding the amino-terminal domain of pIII, a minor coat protein exposed at one end of the phage. Fusion gene was packaged into phagemid particles upon superinfection with M13K07 helper phage. Expression of IFN-alpha lc/86D was verified by its reactivity with IFN-alpha l specific neutralizing antibodies and the phage displayed IFN-alpha lc86D was found to possess antiviral biological activity on VSV challenged WISH cells comparable to that of human recombinant IFN-alpha lb. These results demonstrate that IFN-alpha lc/86D can be displayed on phage in a correctly folded and functionally active form, and this system can be applicable to the sorting of a large repertoire of phage-displayed IFN-alpha lc/86D variants.
Ma
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State Key Laboratory for Molecular Virology and Genetic Engineering, Beijing 100052.
Hu
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R
Lu
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H
D000998:Antiviral Agents; D001435:Bacteriophages / Q000235:genetics*; D020650:Combinatorial Chemistry Techniques; D004274:DNA, Recombinant / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis* / Q000235:genetics; D016898:Interferon-alpha / Q000096:biosynthesis* / Q000235:genetics; D019151:Peptide Library; D011994:Recombinant Proteins
D000998:Antiviral Agents; D004274:DNA, Recombinant; D007370:Interferon Type I; D016898:Interferon-alpha; D019151:Peptide Library; D011994:Recombinant Proteins