Expanded bed adsorption processing of mammalian cell culture fluid: comparison with packed bed affinity chromatography.
A comparison between expanded bed adsorption and conventional packed bed Protein A Fast Flow to purify the anti-rHBsAg mAbs from feedstock is presented in this work. Direct capture by STREAMLINE expanded bed adsorption chromatography resulted in 92% product recovery and sevenfold more concentrated product with similar purity levels compared to that obtained by the standard packed method. The process time and buffer consumption were reduced in the expanded bed adsorption method not only with the binding-elution conditions but also with the use of NaOH during the cleaning-in-place step. The latter is the most widely accepted agent in downstream processing, being a cost effective technique that provides not only efficient cleaning but also sanitizes complete column systems and destroys pirogens.
González
Y
Y
Monoclonal Antibodies Department, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, 10600, Havana, Cuba.
Ibarra
N
N
Gómez
H
H
González
M
M
Dorta
L
L
Padilla
S
S
Valdés
R
R
D000327:Adsorption; D000818:Animals; D018929:Cell Culture Techniques; D002846:Chromatography, Affinity / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods