Identification of a wheat allergen, Tri a Bd 36K, as a peroxidase.
A 36-kDa allergen, Tri a Bd 36K, was purified from wheat albumin and characterized. The protein was similar to barley peroxidase BP-1 both in its amino acid sequence and peroxidase activity. The enzyme seemed to contain L-fucose and D-mannose and the glycan moiety reacted with IgE antibodies in a patient's serum.
Yamashita
Hiromi
H
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja-shi, Okayama 719-1197, Japan. yamashit@fhw.oka-pu.ac.jp
Nanba
Yoko
Y
Onishi
Miki
M
Kimoto
Masumi
M
Hiemori
Miki
M
Tsuji
Hideaki
H
D000485:Allergens / Q000032:analysis*; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D005643:Fucose / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000097:blood; D037102:Lectins / Q000097:blood; D008358:Mannose / Q000378:metabolism; D008970:Molecular Weight; D010544:Peroxidases / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D011134:Polysaccharides / Q000378:metabolism; D011817:Rabbits; D014908:Triticum / Q000201:enzymology*
D000485:Allergens; D000906:Antibodies; D037102:Lectins; D011134:Polysaccharides; C064549:lectin, Aleuria aurantia; D005643:Fucose; D007073:Immunoglobulin E; D006735:Horseradish Peroxidase; D010544:Peroxidases; C070881:barley peroxidase; D008358:Mannose