[Nuclear ribosomal RNA small subunit (18S rRNA) nucleotide sequencing and characterization of sailonggu(whole bone of Myospalax baileyi Thomas)].
OBJECTIVE
Sequencing the nuclear ribosomal RNA small subunit (18S rRNA) gene of Myospalax baileyi (Cricetidae) to develop an ultimate and definitive means for origin identification of genuine Sailonggu.


METHODS
The total DNA was prepared from dried tail tissues. The nuclear 18S rRNA gene region was amplified by PCR using a consensus primer set and its nucleotide sequence was determined by PCR direct sequencing. The characteristic analysis of 18S rRNA sequences was generated using software program Genetyx-SV/R Version 10.1.


RESULTS
The entire 18S rRNA gene region of M. baileyi spanded 1851 bp in length. Although multiple alignment of sequence indicates that there are only lower homology (72.04%-72.18%) comparing with its two alias Mus musculus (GenBank Accession number X00686) and Rattus norvegicus (M11188) (Muridae), their highly conservative domain is located in 1020-1509 nt. There are many variable sites from upstream of 5'-end, which could provide a novel information for molecular recognition of Sailonggu.


CONCLUSIONS
DNA sequencing could be a useful and reliable tool in the origin identification of genuine Sailonggu.
Cao
H
H
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Liu
Y P
YP
Zhang
S L
SL
Zhou
K Y
KY
D000818:Animals; D003411:Arvicolinae / Q000235:genetics*; D001483:Base Sequence; D001842:Bone and Bones / Q000737:chemistry; D004340:Drug Contamination; D008419:Materia Medica / Q000737:chemistry; D008969:Molecular Sequence Data; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D051381:Rats; D017423:Sequence Analysis, RNA
D008419:Materia Medica; D012337:RNA, Ribosomal, 18S