title: Analysis of transcription of plant 7SL RNA gene variants in HeLa in vitro transcription system.
abstract: We have performed an analysis of transcription of hop 7SL RNA genes in heterologous human extract from HeLa cells. Several variants of the Hl7SL-1 gene with a truncated or mutated 5' non-transcribed part revealed a crucial importance of TATA box for transcription. The USE element was found important but dispensable for transcription. Transcription of mutants in the A-like box and experiments with hop 7SL RNA pseudogenes E44 and G32 revealed the importance of internal elements. The A-like box and possibly CG doublet at position +15/+16 described by Bredow et al. (1990a) are according to our results indispensable for in vitro transcription of plant 7SL RNA genes in human extract.
authors.affiliation: Department of Molecular Genetics, Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Ceské Budejovice, Czech Republic.
authors.forename: L
authors.initials: L
authors.lastname: Vrba
authors.forename: J
authors.initials: J
authors.lastname: Matousek
mesh_terms: D001483:Base Sequence; D006367:HeLa Cells; D006801:Humans; D027582:Humulus / Q000235:genetics*; D008969:Molecular Sequence Data; D020733:RNA, Small Cytoplasmic / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D016415:Sequence Alignment; D018271:Signal Recognition Particle / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: C115990:7SL RNA; D020733:RNA, Small Cytoplasmic; D018271:Signal Recognition Particle