Mast cells produce interleukin-25 upon Fc epsilon RI-mediated activation.
Interleukin-25 (IL-25) is a recently described T helper 2 (T(H)2) cell-derived cytokine that belongs to the IL-17 family and induces the production of IL-4, IL-5, and IL-13 from an unidentified non-T-cell population. Here, we show that mast cells are also potent IL-25-producing cells. When bone marrow-derived mast cells were stimulated by immunoglobulin E cross-linking, IL-25 mRNA was induced within 30 minutes in a calcineurin-dependent manner, and the levels of IL-25 mRNA were comparable with those of activated T(H)2 cells. Production of IL-25 by mast cells was also detected at protein levels by immunoblotting. These results suggest that mast cells may enhance T(H)2-type immune response by producing IL-25.
Ikeda
Kei
K
Department of Internal Medicine II, Chiba University School of Medicine, Chiba, Japan.
Nakajima
Hiroshi
H
Suzuki
Kotaro
K
Kagami
Shin-ichiro
S
Hirose
Koichi
K
Suto
Akira
A
Saito
Yasushi
Y
Iwamoto
Itsuo
I
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D019703:Calcineurin / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects*; D007073:Immunoglobulin E / Q000494:pharmacology*; D020381:Interleukin-17 / Q000096:biosynthesis / Q000235:genetics; D007378:Interleukins / Q000096:biosynthesis* / Q000235:genetics; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis; D017455:Receptors, IgE / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018418:Th2 Cells / Q000378:metabolism
C440021:IL17F protein, human; C486701:IL25 protein, human; D020381:Interleukin-17; D007378:Interleukins; C442281:Mydgf protein, mouse; D012333:RNA, Messenger; D017455:Receptors, IgE; D007073:Immunoglobulin E; D000001:Calcimycin; D019703:Calcineurin; D013755:Tetradecanoylphorbol Acetate