title: [Effect of a proteinase-activated receptor-2 (PAR-2) agonist on tryptase release from human mast cells].
abstract: Proteinase-activated receptor-2 (PAR-2) expression has been observed on numerous cell types. However, little is known about the functional expression of PAR-2 in human mast cells. In the current study, the actions of a PAR-2 agonist trans-cinnamoyl-Leu-Ile-Gly-Arg-Leu-Orn-amide (tc-LIGRLO) on tryptase release from dispersed human colonic mast cells were examined. The results showed that tc-LIGRLO was able to induce a fold increase in tryptase release over the basal level following a 15 min incubation of colonic mast cells, whereas tc-OLRGIL did not have any effect on tryptase release. The potency of tc-LIGRLO appeared greater than that of anti-IgE and calcium ionophore A23187 (CI) in induction of tryptase release. Extending the incubation time to 30 min had no significant effect on the actions of tc-LIGRLO or anti-IgE. In the time course study, it was observed that the tryptase release from mast cells induced by tc-LIGRLO started at 1 min and peaked at 3 min following incubation. The above-mentioned results indicate that tc-LIGRLO is a potent stimulus of tryptase release from human mast cells, which strongly suggests that PAR-2s are expressed in human mast cells.
authors.affiliation: Allergy and Inflammation Research Institute, the Medical College of Shantou University, Shantou 515031. shaohenghe@hotmail.com
authors.forename: Shao-Heng
authors.initials: SH
authors.lastname: He
authors.forename: Hua
authors.initials: H
authors.lastname: Xie
authors.forename: Yong-Song
authors.initials: YS
authors.lastname: He
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D044464:Receptor, PAR-2 / Q000819:agonists*; D053802:Tryptases / Q000378:metabolism*
chemical_list: D044464:Receptor, PAR-2; D053802:Tryptases