Morphology of spermatozoa used in IVF and ICSI from oligozoospermic men.
The purpose of this study was to determine morphology in human spermatozoa from men with oligozoospermia and correlate with the fertilization rate, embryo score and pregnancy rate after IVF and intracytoplasmic sperm injection (ICSI) respectively. The study group consisted of 125 couples where the male partner suffered from oligozoospermia. Fifty of these had IVF (group A). Seventy-five couples in whom ICSI had been performed made up group B. Sperm samples were assessed according to the WHO manual. For each male, morphology of spermatozoa was judged according to Krüger's strict criteria, WHO criteria and the teratozoospermia index (TZI). Oocyte monitoring was carried out according to a long down-regulation protocol using gonadotrophin-releasing hormone, recombinant FSH and human chorionic gonadotrophin. Embryos were transferred on day 2 after fertilization, with a maximum of three embryos. This study demonstrated no correlation between any of the morphological assessments of spermatozoa and the fertilization rate, embryo score and pregnancy rate, either after IVF or ICSI. Morphology in human spermatozoa according to Krüger's strict criteria, WHO criteria and the TZI had no predictive value for the outcome after either IVF or ICSI.
Høst
Erik
E
Ciconia Research and Development Aps, Symbion Fruebjergvej 3, DK-2100, Denmark.
Ernst
Erik
E
Lindenberg
Svend
S
Smidt-Jensen
Steen
S