Response regulator ChiR regulates expression of chitinase gene, chiC, in Streptomyces coelicolor.
Transcription from the chiC promoter, directing expression of the chitinase gene, chiC, in Streptomyces coelicolor, was analyzed using xylE reporter gene and high-resolution S1-nuclease mapping. The transcription from the chiC promoter was induced by chitin, and this induction was dramatically reduced in the S. coelicolor chiR-disrupted strain. This indicated a dependence of chiC expression upon the chiR gene encoding a response regulator protein. To investigate this relationship, the S. coelicolor ChiR was overproduced using Escherichia coli T7 RNA polymerase expression system. However, gel mobility shift-assay with such a purified ChiR showed no binding in the chiC promoter region, which indicates a lack of specific phosphorylation of E. coli overproduced ChiR that is necessary for DNA-binding activity of response regulators.
Homerová
D
D
Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovakia.
Knirschová
R
R
Kormanec
J
J
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002688:Chitinases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D014157:Transcription Factors; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C403318:ChiR protein, Streptomyces; D014157:Transcription Factors; D015719:Single-Strand Specific DNA and RNA Endonucleases; D002688:Chitinases; C101644:chitinase C-1