title: Lipase activity and gene cloning of Acinetobacter calcoaceticus LP009.
abstract: The production of lipase from Acinetobacter calcoaceticus LP009, a bacterium isolated from raw milk, was found to be best induced by Tween-80 at 1.0% concentration. It was efficiently secreted, and only a minute amount of activity was detected at the cell surface and intracellularly. A. calcoaceticus LP009 lipase exhibited maximum activity at pH 7.0 and 50 degrees C, and was relatively stable upon storage at pH 5.0 to 7.0 and at 4, 30, or 37 degrees C. The enzyme was found to be inactivated by EDTA suggesting that it was a metalloenzyme. Its activity was reduced by less than 20% with the addition of various ions to reaction mixtures, but long storage with them caused approximately 50% reduction in subsequent reactions under standard conditions. By contrast, the addition of Fe(3+) enhanced activity. The enzyme was highly stable upon storage with 0.1% of Triton X-100, Tween-80 or Tween-20, but highly unstable with various organic solvents tested. PMSF, a serine enzyme inhibitor, and 2-mercaptoethanol, a reducing agent, did not affect enzyme activity. After extraction and transfer, the lipase gene was efficiently expressed in recombinant Aeromonas sobria. This recombinant strain was shown to have increased hydrolyzing efficiency and have high potential for lipid-rich wastewater treatment.
authors.affiliation: Center for Biotechnology, Institute for Research and Development in Science and Technology, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.
authors.forename: Saovanee
authors.initials: S
authors.lastname: Dharmsthiti
authors.forename: Jaturong
authors.initials: J
authors.lastname: Pratuangdejkul
authors.forename: Gunjana Theeragool
authors.initials: GT
authors.lastname: Theeragool
authors.forename: Sudaporn
authors.initials: S
authors.lastname: Luchai