title: Multianalyte pin-printed biosensor arrays based on protein-doped xerogels.
abstract: We report the first biosensor arrays based on pin printing protein-doped xerogels. The individual biosensor elements are on the order of 100 microm in diameter. Arrays are formed (1) onto a planar substrate that is excited by an external source (laser) or (2) directly on the face of a light-emitting diode. We illustrate the potential of our approach by fabricating, testing, and characterizing four types of pin-printed biosensor arrays (PPBSA) for the simultaneous detection of glucose and O2. The analytically reliable operating ranges for the PPBSAs are 0.1-10 mM for glucose and 0.1-100% for O2. The PPBSAs exhibit short- and long-term reproducibilities of no worse than 4 and 8%, respectively. The overall array-to-array response reproducibilities are < or = 12%. These results demonstrate for the first time the combination sol-gel processing and pin printing methods as a way to rapidly form ensembles of integrated, reusable, and stable biosensor arrays for simultaneous multianalyte detection.
authors.affiliation: Department of Chemistry, Natural Sciences Complex, University at Buffalo, The State University of New York, Buffalo, New York 14260-3000, USA.
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Cho
authors.forename: Zunyu
authors.initials: Z
authors.lastname: Tao
authors.forename: Elizabeth C
authors.initials: EC
authors.lastname: Tehan
authors.forename: Frank V
authors.initials: FV
authors.lastname: Bright
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D005782:Gels / Q000737:chemistry; D005947:Glucose / Q000032:analysis; D010100:Oxygen / Q000032:analysis; D011506:Proteins / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D005782:Gels; D011506:Proteins; D005947:Glucose; D010100:Oxygen