title: Human T lymphocyte response to Borrelia burgdorferi infection: no correlation between human leukocyte function antigen type 1 peptide response and clinical status.
abstract: We tested the hypothesis that cross-reactivity between the outer surface protein A (OspA) of Borellia burgdorferi and human leukocyte function antigen (LFA) type 1 mediates chronic autoimmune sequelae of Lyme disease. T cell response was studied in subjects with Lyme disease presenting with erythema migrans alone (n=36), erythema migrans with neurological disease (n=12), and chronic Lyme disease syndrome (n=20), as well as healthy control subjects from Lyme-endemic (n=50) and -nonendemic (n=18) regions. Antigens included recombinant OspA and OspC (all strain B31) and human LFA-1 peptide (IYVIEGTSKQDLTSF). Proliferation to OspA was detected in 11 (28%) of 39 of subjects presenting with erythema migrans, which increased to 50% at 4 weeks of follow-up. Reactivity to OspA and LFA-1 was significantly correlated (P<.001) and was observed in 18 (78%) of 23 of OspA-responsive subjects. However, there was no correlation between T cell response to human LFA-1 peptide and clinical status.
authors.affiliation: Department of Dermatology, State University of New York at Stony Brook, 11794-8165, USA. richard.kalish@stonybrook.edu
authors.forename: Richard S
authors.initials: RS
authors.lastname: Kalish
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Wood
authors.forename: William
authors.initials: W
authors.lastname: Golde
authors.forename: Robert
authors.initials: R
authors.lastname: Bernard
authors.forename: Larry E
authors.initials: LE
authors.lastname: Davis
authors.forename: Roger C
authors.initials: RC
authors.lastname: Grimson
authors.forename: Patricia K
authors.initials: PK
authors.lastname: Coyle
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Luft
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial; D000954:Antigens, Surface / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001428:Bacterial Vaccines; D003429:Cross Reactions; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D008074:Lipoproteins; D008193:Lyme Disease / Q000276:immunology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology*; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D008074:Lipoproteins; D016169:Lymphocyte Function-Associated Antigen-1; C072045:OspA protein; C079616:OspC protein; D007371:Interferon-gamma