title: Development of 47 new microsatellite markers from a BTA6 library.
abstract: Chromosome-specific libraries provide a means to isolate genetic markers from specific chromosomal regions. A small-insert BTA6 library, constructed by microdissection, was screened for dinucleotide repeats (CA)15 and (GA)15. A total of 47 new microsatellite loci were developed and tested for polymorphism and informativeness using the MARC bovine mapping family.
authors.affiliation: Department of Veterinary PathoBiology, University of Minnesota, St. Paul, MN, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Reed
authors.forename: N
authors.initials: N
authors.lastname: Ihara
authors.forename: F A
authors.initials: FA
authors.lastname: Ponce de León
authors.forename: T S
authors.initials: TS
authors.lastname: Sonstegard
authors.forename: T P L
authors.initials: TP
authors.lastname: Smith
authors.forename: G L
authors.initials: GL
authors.lastname: Bennett
authors.forename: Y
authors.initials: Y
authors.lastname: Sugimoto
authors.forename: C W
authors.initials: CW
authors.lastname: Beattie
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping / Q000662:veterinary; D033481:Chromosomes, Mammalian / Q000235:genetics*; D015723:Gene Library; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000235:genetics