title: [Hepatitis B virus antigen-induced specific TCR V beta gene subfamily amplifications and their diversity].
abstract: The human immune system confronts various antigens, then the recognitive structures of T lymphocytes must be diverse. In this study, we analyzed the TCR V beta 1-20 gene repertoire by reverse transcription-polymerase chain reaction (RT-PCR) in peripheral blood lymphocytes (PBLs) from individuals vaccinated with recombinant hepatitis B vaccine and PBLs from healthy donors stimulated by supernatants of the HepG2 2215 cell line transfected by HBV-DNA. The specific amplifications of V beta 6, 14, V beta 6, 15 were observed in PBLs after vaccination and stimulation by HepG2 2215 cell line respectively. The results suggest that the V beta segments may be the specific recognitive units for these antigens and involved in restriction and cytotoxicity.
authors.affiliation: Department of Molecular Biology, Guangdong Pharmacy College, Guangzhou 510224.
authors.forename: S
authors.initials: S
authors.lastname: Huang
authors.forename: M
authors.initials: M
authors.lastname: Cao
authors.forename: L
authors.initials: L
authors.lastname: Luo
mesh_terms: D002478:Cells, Cultured; D019674:Genes, T-Cell Receptor beta / Q000235:genetics*; D014644:Genetic Variation; D006514:Hepatitis B Surface Antigens / Q000494:pharmacology*; D017325:Hepatitis B Vaccines / Q000276:immunology; D006513:Hepatitis B e Antigens / Q000494:pharmacology*; D006515:Hepatitis B virus / Q000276:immunology; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D014407:Tumor Cells, Cultured; D014611:Vaccination
chemical_list: D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D006513:Hepatitis B e Antigens