[Effects of curcumin on proliferation and apoptosis in human hepatic cells].
OBJECTIVE
To investigate the effect of curcumin on the proliferation, cell cycle distribution and apoptosis of hepatocarcinoma cell line QGY.


METHODS
The MTT method was used to assay the biologic activities of curcumin in different times and different doses. The cell cycle distribution was detected by flow cytometic analysis. The cell ultrastructure was observed by electronic microscopy.


RESULTS
Curcumin could inhibit effectively QGY in a dose- and time- dependent manner. IC(50) of curcumin to QGY was 49.50 micromol/L in 72 hours. The cell growth was arrested at S stage. Curcumin could lead to the degeneration, necrosis, and apoptosis.


CONCLUSIONS
Curcumin can interrupt the cell cycle and has a role in cytotoxicity, antiproliferation and inducing apoptosis of QGY.
Li
Hongyuan
H
Department of General Surgery, First Affiliated Hospital of Chongqing University of Medical Sciences, China.
Che
Yi
Y
Tang
Weixue
W
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000648:ultrastructure; D008854:Microscopy, Electron; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000648:ultrastructure
D000970:Antineoplastic Agents; D003474:Curcumin