Acute phase effects of ATP-MgCl2 on experimental spinal cord injury.
The purpose of this study was to study the acute phase effects of adenosine triphosphate (ATP)-MgCl2 on experimental spinal cord clip compression injury. Spinal cord clip compression injury was performed on 36 albino Wistar rats. The rats were divided into five groups. T4-T8 total laminectomy was performed on all rats. Group 1: sham-operated group. Group 2: clip compression group. In group 3, ATP-MgCl2 (100 micro mol/kg) was given 2 min before the "clip compression injury." In group 4, ATP-MgCl2 (100 micro mol/kg) was given 5 min after the clip compression injury. In group 5, ATP MgCl2 (100 micro mol/kg) was administered 8 h after the injury. The spinal cords were excised for a length of 2 cm and deep frozen at -76 degrees C. Tissue malondialdehyde (MDA) levels were used to determine the effects of ATP-MgCl2 on spinal cord lipid peroxidation. In the groups in which ATP MgCl2 was administered after the clip compression injury (groups 4 and 5), the decrease in spinal cord MDA levels was statistically significant when compared with those of the injury group (group 2). Although MDA levels of group 4 were lower than those of group 5, this difference was not statistically significant. Administration of the ATP-MgCl2 before the clip compression injury (in group 3) did not have a statistically significant effect on lipid peroxidation when compared with the injury group (group 2). In this study, we found that ATP-MgCl2 has decreased lipid peroxidation in spinal cord injury and protected the spinal cord from secondary injury after the trauma. We concluded that ATP-MgCl2 may be used in the treatment of spinal cord injuries in conjunction with the other treatment modalities, but further investigations are mandatory.
Cakir
Ertugrul
E
Department of Neurosurgery, KTU Farabi Hospital, 61080 Trabzon, Turkey. ertugrulcakir@hotmail.com
Baykal
Suleyman
S
Karahan
S Caner
SC
Kuzeyli
Kayhan
K
Uydu
Huseyin
H
D000210:Acute-Phase Reaction / Q000188:drug therapy* / Q000209:etiology*; D000255:Adenosine Triphosphate / Q000627:therapeutic use*; D000818:Animals; D004195:Disease Models, Animal; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000737:chemistry; D013117:Spinal Cord Compression / Q000150:complications* / Q000188:drug therapy*; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000209:etiology*; D013997:Time Factors
D008315:Malondialdehyde; D000255:Adenosine Triphosphate