[Studies on separation and identification of Rhizoma coptidis brugs based on microemulsion thin-layer chromatography].
OBJECTIVE
To investigate the use of microemulsion TLC for the separation and identification of Rhizoma Coptidis drugs.


METHODS
Thirteen Rhizoma Coptidis drugs were separated and identified with six kinds of SDS/n-C4H9OH/n-C7H16/H2O microemulsions as mobile phase on polyamide film. Effects of microemulsions on separation were investigated.


RESULTS
The best mobile phase was the O/W microemulsion(containing 75% water). Compared with general mobile phases, the separation and detection could be improved and the test conditions were more tolerant.


CONCLUSIONS
This new method is simple, accurate and efficient for the separation and identification of Rhizoma Coptidis drugs.
Kang
C
C
Yangzhou Municipal Institute for Drug Control, Yangzhou 225009, Jiangsu, China.
Wen
L Y
LY
Ding
Z B
ZB
Guo
R
R
Zhu
X S
XS
D001599:Berberine / Q000032:analysis; D001600:Berberine Alkaloids / Q000032:analysis; D002855:Chromatography, Thin Layer / Q000379:methods; D000089042:Coptis chinensis; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry* / Q000145:classification; D029626:Ranunculaceae / Q000737:chemistry* / Q000145:classification
D001600:Berberine Alkaloids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D001599:Berberine; C107393:Coptidis rhizoma extract; C005413:palmatine