[Effects of bone morphogenetic protein on proliferation and collagen-1 synthesis of skeletal muscle satellite cells].
OBJECTIVE
To investigate the effects of bone morphogenetic protein (BMP) on the proliferation and collagen synthesis of skeletal muscle satellite cells.


METHODS
Skeletal muscle satellite cells were harvested and cultured in vitro. The 0 ng/ml, 50 ng/ml, 100 ng/ml, 500 ng/ml, and 1000 ng/ml BMP were used to induce skeletal muscle satellite cells for 48 hours. Cell proliferation, rate of myotube formation and collagen-1 synthesis were measured.


RESULTS
BMP promoted cell proliferation and reduced the rate of myotube formation. Collagen synthesis increased when skeletal muscle satellite cells were induced with more than 500 ng/ml BMP. And the higher the concentration of BMP was, the stronger this effect became.


CONCLUSIONS
BMP can enhance the proliferation of skeletal muscle satellite cells and change their differentiation from myoblasts to osteoblasts.
Wei
Kuan-hai
KH
Department of Traumutology and Orthopedics, Nanfang Hospital, First Military Medical University, Guangzhou Guangdong, P. R. China 510515.
Pei
Guo-xian
GX
Jin
Dan
D
D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002459:Cell Fusion; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D010012:Osteogenesis; D051381:Rats; D017208:Rats, Wistar; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000187:drug effects*
D019485:Bone Morphogenetic Proteins; D003094:Collagen