title: Response regulator ChiR regulates expression of chitinase gene, chiC, in Streptomyces coelicolor.
abstract: Transcription from the chiC promoter, directing expression of the chitinase gene, chiC, in Streptomyces coelicolor, was analyzed using xylE reporter gene and high-resolution S1-nuclease mapping. The transcription from the chiC promoter was induced by chitin, and this induction was dramatically reduced in the S. coelicolor chiR-disrupted strain. This indicated a dependence of chiC expression upon the chiR gene encoding a response regulator protein. To investigate this relationship, the S. coelicolor ChiR was overproduced using Escherichia coli T7 RNA polymerase expression system. However, gel mobility shift-assay with such a purified ChiR showed no binding in the chiC promoter region, which indicates a lack of specific phosphorylation of E. coli overproduced ChiR that is necessary for DNA-binding activity of response regulators.
authors.affiliation: Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovakia.
authors.forename: D
authors.initials: D
authors.lastname: Homerová
authors.forename: R
authors.initials: R
authors.lastname: Knirschová
authors.forename: J
authors.initials: J
authors.lastname: Kormanec
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002688:Chitinases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D014157:Transcription Factors; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; C403318:ChiR protein, Streptomyces; D014157:Transcription Factors; D015719:Single-Strand Specific DNA and RNA Endonucleases; D002688:Chitinases; C101644:chitinase C-1