Antibodies present in normal human serum inhibit invasion of human brain microvascular endothelial cells by Listeria monocytogenes.
Listeria monocytogenes causes meningitis and encephalitis in humans and crosses the blood-brain barrier by yet unknown mechanisms. The interaction of the bacteria with different types of endothelial cells was recently analyzed, and it was shown that invasion into, but not adhesion to, human brain microvascular endothelial cells (HBMEC) depends on the product of the inlB gene, the surface molecule InlB, which is a member of the internalin multigene family. In the present study we analyzed the role of the medium composition in the interaction of L. monocytogenes with HBMEC, and we show that invasion of HBMEC is strongly inhibited in the presence of adult human serum. The strong inhibitory activity, which is not present in fetal calf serum, does not inhibit uptake by macrophage-like J774 cells but does also inhibit invasion of Caco-2 epithelial cells. The inhibitory component of human serum was identified as being associated with L. monocytogenes-specific antibodies present in the human serum. Human newborn serum (cord serum) shows only a weak inhibitory activity on the invasion of HBMEC by L. monocytogenes.
Hertzig
Tobias
T
Lehrstuhl für Mikrobiologie, Theodor-Boveri-Institut für Biowissenschaften der Universität Würzburg, Würzburg, Germany.
Weber
Martin
M
Greiffenberg
Lars
L
Holthausen
Britta Schulte
BS
Goebel
Werner
W
Kim
Kwang Sik
KS
Kuhn
Michael
M
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology*; D001422:Bacterial Adhesion; D001769:Blood / Q000276:immunology; D001921:Brain / Q000098:blood supply*; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D004730:Endothelium, Vascular / Q000382:microbiology*; D005312:Fetal Blood / Q000276:immunology; D006801:Humans; D008089:Listeria monocytogenes / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology; D008833:Microcirculation / Q000382:microbiology; D008855:Microscopy, Electron, Scanning
D000907:Antibodies, Bacterial; D003470:Culture Media