[Low frequency of precore stop mutation in patients with dual infection of hepatitis B and C viruses].
In order to study the mutation of hepatitis B virus(HBV) precore gene in patients with dual infections of HBV and HCV and its possible clinical significance, the precore stop codon mutation (stop 28) was analysed by polymerase chain reaction (PCR) and restriction fragment length polymorphism(RFLP) in patients with chronic liver disease, which were 25 cases with HBV DNA and HCV RNA positive(group A) and 31 cases with HBsAg and HBV DNA positive but anti-HCV and HCV RNA negative (group B). The results showed that the serum HBV DNA positive rate by 1st PCR in patients with dual infections(group A, 16%) was significantly lower than that in patients with HBV infection alone (group B, 65%), the stop 28 positive rate was also significantly lower (28%, versus 68%). The results suggest that the low frequency of precore stop codon mutant in patients with HBV and HCV dual infections was possibly due to low replication of HBV.
Qiu
W
W
Department of Infectious Disease, Guangzhou Railway Central Hospital, Guangzhou 510080.
Hou
J
J
Lou
K
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D018388:Codon, Terminator / Q000235:genetics; D005260:Female; D016174:Hepacivirus / Q000235:genetics; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics*; D006526:Hepatitis C / Q000821:virology*; D006801:Humans; D008070:Lipopolysaccharides; D008297:Male; D008875:Middle Aged; D009154:Mutation; D015163:Superinfection / Q000821:virology*; D014758:Viral Core Proteins / Q000235:genetics; D014779:Virus Replication
D018388:Codon, Terminator; D008070:Lipopolysaccharides; C085483:Re lipopolysaccharide; D014758:Viral Core Proteins