[Serologic testing of hepatitis C virus infection].
We tested anti-HCV, anti-HCV IgM and HCV-RNA in serum samples from general adults, blood donors, acute hepatitis patients and liver cirrhosis patients by the methods of ELISA and RT-PCR so as to study the relationship among serum markers. Results showed that the detected positive rates of anti-HCV are 3.57%, 8.58%, 6.25% and 48.38% in normal adults, blood donors, acute hepatitis patients and liver cirrhosis patients respectively; agreement rates between anti-HCV and HCV RNA are 11.43%, 61.11%, 80.0%, and 73.33% in the four groups of people above; agreement rates between anti-HCV IgM and HCV RNA are 75.0%, 90.9% 81.81% and 100% in the same groups of people. Authors pointed out that anti-HCV IgM is superior to anti-HCV in the aspect of revealing HCV viremia. In comparison with RT-PCR, testing anti-HCV IgM is easier and does not need special instruments, so it is suitable to develop in the basic hygiene units. Testing anti-HCV IgM instead of anti-HCV, the morbidity of post-transfusion hepatitis C can be decreased further.
Li
S
S
Jilin Provincial Antiepidemic Station, Changchun 130021.
Wu
Y
Y
Hu
G
G
D000914:Antibodies, Viral / Q000097:blood*; D002353:Carrier State / Q000276:immunology / Q000821:virology; D016174:Hepacivirus / Q000276:immunology / Q000302:isolation & purification*; D006526:Hepatitis C / Q000276:immunology / Q000821:virology*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D012367:RNA, Viral / Q000097:blood*
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D012367:RNA, Viral