title: Contribution of ferrous iron to maintenance of the gastric colonization of Helicobacter pylori in miniature pigs.
abstract: Our previous study showed that the colonization levels of Helicobacter pylori were higher in the stomachs of 5-day-old miniature pigs than in 2-week-old ones. As dietary factors can cause these differences, we compared two diets, i.e., Weanymilk and a similar formula with a higher concentration of Fe(II), Weanylobulin. The colonization levels in the fundic mucosa were significantly higher in 2-week-old pigs fed Weanylobulin than in those fed Weanymilk. Supplementing Weanylobulin with an iron chelator, deferoxamine mesylate, significantly lowered the bacteria counts in the gastric mucosa. Normal diets supplemented with Fe(II) in 2-month-old pigs caused significantly more sites of bacteria in the antrum compared with normal diets alone. In addition, ranitidine, an inhibitor of gastric acid secretion that reduces Fe(III) to Fe(II) in the stomach, decreased the bacteria counts in 10-month-old pigs. These results suggested that Fe(II) maintained the colonization levels of H. pylori in the stomach of the miniature pigs.
authors.affiliation: Biological Research Laboratories, Sankyo Co., Ltd., Shinagawa-ku, Tokyo, Japan. tekoga@shina.sankyo.co.jp
authors.forename: Tetsufumi
authors.initials: T
authors.lastname: Koga
authors.forename: Yukio
authors.initials: Y
authors.lastname: Shimada
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Sato
authors.forename: Keigo
authors.initials: K
authors.lastname: Takahashi
authors.forename: Isamu
authors.initials: I
authors.lastname: Kikuchi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Okazaki
authors.forename: Tomoko
authors.initials: T
authors.lastname: Miura
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Katsuta
authors.forename: Masayuki
authors.initials: M
authors.lastname: Iwata
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D003676:Deferoxamine / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000382:microbiology*; D016480:Helicobacter pylori / Q000254:growth & development*; D007505:Iron-Dextran Complex / Q000494:pharmacology; D008297:Male; D011899:Ranitidine / Q000494:pharmacology; D013552:Swine; D013556:Swine, Miniature
chemical_list: D005296:Ferrous Compounds; D011899:Ranitidine; D007505:Iron-Dextran Complex; D003676:Deferoxamine