[Construction of adenovirus type 7(vaccine strain) vector and expression of beta-galactosidase gene].
Human adenovirus type 7 DNA was extracted from purified virus cultured in WI-38 cells. The essential fragment (68-100 mu) of Ad7 DNA was used for constructing a non-defective Ad7 vector termed as pAd7 delta E3. The vector was characterized by a deletion at the E3 region that contains a multicloning site for the insertion of foreign genes. A helper-independent adenovirus type 7-beta-galactosidase recombinant was established. The recombinant contains the beta-galactosidase gene flanked by CMV early promoter and SV40 poly A signal. The constructed recombinant virus showed efficient capacity in expressing the foreign gene, beta-galactosidase.
Shi
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Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052.
Wen
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Liu
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D017503:Adenovirus E3 Proteins / Q000235:genetics*; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics*; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D011061:Poly A; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics; D014765:Viral Vaccines; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000235:genetics
D017503:Adenovirus E3 Proteins; D004279:DNA, Viral; D011994:Recombinant Proteins; D014765:Viral Vaccines; D011061:Poly A; D001616:beta-Galactosidase