Mutual binding inhibition of tetrodotoxin and saxitoxin to their binding protein from the plasma of the puffer fish, Fugu pardalis.
The mutual binding inhibition of tetrodotoxin and saxitoxin to their binding protein from the plasma of Fugu pardalis was investigated by HPLC. The values for the half inhibitory concentration of tetrodotoxin (1.6 microM) binding to this protein (1.2 microM) for saxitoxin, and of saxitoxin (0.47 microM) binding to that (0.30 microM) for tetrodotoxin were 0.35 +/- 0.057 microM and 81 +/- 16 microM (n = 2), respectively.
Yotsu-Yamashita
Mari
M
Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan. myama@biochem.tohoku.ac.jp
Shoji
Yuki
Y
Terakawa
Takahiro
T
Yamada
Shunichi
S
Miyazawa
Teruo
T
Yasumoto
Takeshi
T
D000818:Animals; D001667:Binding, Competitive; D001798:Blood Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D020128:Inhibitory Concentration 50; D011485:Protein Binding; D012530:Saxitoxin / Q000037:antagonists & inhibitors / Q000097:blood*; D023721:Tetraodontiformes; D013779:Tetrodotoxin / Q000037:antagonists & inhibitors / Q000097:blood*
D001798:Blood Proteins; D012530:Saxitoxin; D013779:Tetrodotoxin