title: Anti-macrophage inhibitory factor antibody inhibits PMSG-hCG-induced follicular growth and ovulation in mice.
abstract: OBJECTIVE
  To investigate the effect of an anti-MIF antibody on PMSG-hCG-induced murine follicular growth and ovulation and to determine whether MIF plays an essential role in this process.
  
  
  METHODS
  Mice were primed with an intraperitoneal injection of pregnant mare serum gonadotropin (PMSG) and were treated with an anti-rat MIF antibody and human chorionic gonadotropin (hCG) to induce ovulation. After that, the ovulated ova were counted. The ovaries were studied using standard histological procedures.
  
  
  RESULTS
  Ovaries treated with the anti-MIF antibody showed reduced numbers of growing follicles surrounded by granulosa cells and theca cells with a little proliferation compared with the control. The average numbers of ova collected from mice treated with the anti-MIF antibody were reduced compared with those collected from control mice.
  
  
  CONCLUSIONS
  Anti-MIF antibody inhibits the follicular growth and ovulation in mice, and MIF may play an important role in the inflammatory reactions during follicle growth and ovulation.
authors.affiliation: Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu-shi, Shizuoka 431-3192, Japan. matsuura@hama-med.ac.jp
authors.forename: Toshiki
authors.initials: T
authors.lastname: Matsuura
authors.forename: Motoi
authors.initials: M
authors.lastname: Sugimura
authors.forename: Takayuki
authors.initials: T
authors.lastname: Iwaki
authors.forename: Ryota
authors.initials: R
authors.lastname: Ohashi
authors.forename: Naohiro
authors.initials: N
authors.lastname: Kanayama
authors.forename: Jun
authors.initials: J
authors.lastname: Nishihira
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D004338:Drug Combinations; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D008263:Macrophage Migration-Inhibitory Factors / Q000037:antagonists & inhibitors / Q000276:immunology*; D051379:Mice; D008813:Mice, Inbred ICR; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D010060:Ovulation / Q000187:drug effects*
chemical_list: D000906:Antibodies; D006063:Chorionic Gonadotropin; D004338:Drug Combinations; D006064:Gonadotropins, Equine; D008263:Macrophage Migration-Inhibitory Factors; C036835:PMSG-HCG