Leptin-induced changes in body composition in high fat-fed mice.
Female C57BL/6J mice were adapted to 10% or 45% kcal fat diets for 8 weeks. Continuous intraperitoneal infusion of 10 micro g of leptin/day from a miniosmotic pump transiently inhibited food intake in low fat-fed but not high fat-fed mice. In contrast, both low and high fat-fed leptin-infused mice were less fat than their phosphate-buffered saline (PBS) controls after 13 days. Leptin infusion inhibited insulin release but did not change glucose clearance in low fat-fed mice during a glucose tolerance test. A single intraperitoneal injection of 30 micro g of leptin inhibited 24-hr energy intake and inhibited weight gain in both low and high fat-fed mice. Insulin responsiveness was improved in high fat-fed mice during an insulin sensitivity test due to an exaggerated elevation of circulating insulin concentrations. Thus, leptin infusion reduced adiposity independently of energy intake in high fat-fed mice and improved insulin sensitivity in low fat-fed mice, whereas leptin injections, which produced much greater, but transient, increases in serum leptin concentration, inhibited energy intake in both low and high fat-fed mice.
Harris
Ruth B S
RB
Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA. harrisrb@arches.uga.edu
Mitchell
Tiffany D
TD
Hebert
Sadie
S
D000818:Animals; D001823:Body Composition; D001835:Body Weight; D004041:Dietary Fats / Q000008:administration & dosage*; D005247:Feeding Behavior; D005260:Female; D005951:Glucose Tolerance Test; D007333:Insulin Resistance; D020738:Leptin / Q000097:blood / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL
D004041:Dietary Fats; D020738:Leptin