[Sequencing and analysis of the NS5 region of HGV/GBV-C gene].
To study the genomic organization of non structural region 5 of HGV in China, 186 nucleotides of NS5 region of HGV were amplified, cloned and sequenced. The data were analyzed with computer programs. The results indicated that the gene sequence homology between 2 HGV isolates in this study was 95.2%. The comparison of these 2 isolates and 3 foreign isolates reported previously showed that the sequence homology was in the range of 84.4% to 92.4%, and was obvious by divergent from the foreign isolates. The results make us consider that HGV may have different genotypes, after a further inspection into the gene sequence of each region of HGV. The 2 isolates were from the sera collected in 1984, therefore the existence of HGV infection in Harbin was retrospectively traced to 1980s.
Wang
Z
Z
Herbin Sanitation and Antiepidemic Station, Harbin 150010.
Ji
K
K
Love
E J
EJ
D001483:Base Sequence; D018178:Flaviviridae Infections / Q000821:virology*; D029407:GB virus C / Q000235:genetics*; D006525:Hepatitis, Viral, Human / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D017361:Viral Nonstructural Proteins / Q000235:genetics*
C096041:NS5 protein, flavivirus; D017361:Viral Nonstructural Proteins