Role of plasminogen activators during healing after uterine serosal lesioning in the rat.
OBJECTIVE
Study the expression pattern of tissue-type (t-PA) and urokinase-type (u-PA) plasminogen activators during uterine serosal healing after lesioning in a rat model.


METHODS
Descriptive study with an adhesion model using reverse transcriptase-polymerase chain reaction (PCR) and immuncytochemical techniques.


METHODS
Academic medical center.


METHODS
None.


METHODS
None.


METHODS
Expression profiles of t-PA and u-PA were examined in the uteroperitoneal adhesion tissues of rat from the time of injury until day 7 after surgery using relative abundance reverse transcriptase-PCR and immunocytochemical techniques.


RESULTS
The t-PA transcript levels dropped in uteroperitoneal tissues by 6 hours after surgery and remained low until day 5. Thereafter, a rapid up-regulation of this transcript was observed at day 7. In contrast, the u-PA transcript levels demonstrated a biphasic profile during the progression of adhesion development with peaks at 2 and 5 days after surgery. Immunocytochemical staining demonstrated expression of these plasminogen activators in the newly formed blood vessels.


CONCLUSIONS
Altered levels of t-PA and u-PA transcripts and its expression in newly formed blood vessels during healing and adhesion development indicate involvement of these plasminogen activators in serosal healing and adhesion development.
Rout
Ujjwal Kumar
UK
C. S. Mott Center for Human Growth and Development, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA. aa4306@wayne.edu
Diamond
Michael P
MP
D000818:Animals; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D007150:Immunohistochemistry; D010532:Peritoneal Diseases / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D010960:Plasminogen Activators / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000267:Tissue Adhesions / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D010959:Tissue Plasminogen Activator / Q000032:analysis / Q000235:genetics / Q000502:physiology; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis / Q000235:genetics / Q000502:physiology; D014591:Uterine Diseases / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D014599:Uterus / Q000601:surgery*; D014945:Wound Healing / Q000502:physiology*
D012333:RNA, Messenger; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D010960:Plasminogen Activators; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator