title: Automated screening procedure for high-throughput generation of antibody fragments.
abstract: In the emerging field of proteomics, there is an urgent need for catcher molecules such as antibodies for detecting the proteome or parts of the proteome in a microarray format. A suitable source for providing a large diversity of binders is obtained by combinatorial libraries, such as phage display libraries of single chain antibody fragments (scFv) or Fab fragments. To find novel binders from the n-CoDeR libraries with a high throughput, we have automated the screening process with robotics. The automated system is configured to screen tens of thousands of clones per day to target antigens in various formats, including peptides and soluble proteins, as well as cell-bound targets; thus, it is well designed to meet demands from the proteomics area.
authors.affiliation: BioInvent Therapeutic AB (publ), Lund, Sweden.
authors.forename: Johan
authors.initials: J
authors.lastname: Hallborn
authors.forename: Roland
authors.initials: R
authors.lastname: Carlsson
mesh_terms: D000941:Antigens / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020650:Combinatorial Chemistry Techniques / Q000295:instrumentation / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods; D005786:Gene Expression Regulation; D007128:Immunoglobulin Fragments / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods; D012371:Robotics / Q000295:instrumentation* / Q000379:methods
chemical_list: D000941:Antigens; D007128:Immunoglobulin Fragments