Upregulation of phospholipase D1 in the spinal cords of rats with clip compression injury.
This study examined phospholipase D 1 (PLD1) expression in the central nervous system following clip compression spinal cord injury (SCI) in Sprague-Dawley rats. After inducing SCI with a vascular clip, the expression of PLD1 in the affected spinal cord was analyzed by Western blot and immunohistochemistry. Western blot analysis showed that the expression of PLD1 gradually increased in the spinal cord on days 0.5, 1, 2, and 4 post injury. Immunohistochemistry showed that some cells, including neurons, astrocytes, and some inflammatory cells, were positive for PLD1 in the lesions at days 1 and 2 post injury. At day 4, the number of PLD1-positive cells in SCI lesions increased, largely matching the increases in ED1-positive macrophages and glial fibrillary acidic protein-positive astrocytes. At this time, macrophages expressed proliferating cell nuclear antigen in addition to PLD1. These results suggest that PLD1 expression is increased in injured spinal cords, and might be involved in the activation and proliferation of macrophages and astrocytes in SCI.
Jung
Kyungsook
K
Department of Veterinary Medicine, College of Agriculture and Life Science, Cheju National University, Jeju 690-756, South Korea.
Min
Do Sik
DS
Sim
Ki-Bum
KB
Ahn
Meejung
M
Kim
Hwanglyong
H
Cheong
Jongtae
J
Shin
Taekyun
T
D000818:Animals; D001253:Astrocytes / Q000201:enzymology / Q000473:pathology; D008264:Macrophages / Q000201:enzymology / Q000473:pathology; D008297:Male; D009474:Neurons / Q000201:enzymology / Q000473:pathology; D010739:Phospholipase D / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013117:Spinal Cord Compression / Q000201:enzymology* / Q000473:pathology*; D013904:Thoracic Vertebrae; D015854:Up-Regulation
D010739:Phospholipase D; C106801:phospholipase D1