Molecular characterization of voltage-gated sodium channels in human gliomas.
Voltage-sensitive sodium channels appear to be an electrophysiological hallmark of gliomas. However, the expression of channel subtypes is unclear in these tumors. In this study different gliomas were investigated for the expression of sodium channel subtypes Na(v)1.1, Na(v)1.2, Na(v)1.3, Na(v)1.4, Na(v)1.6, and Na(x)(Na(v)2.1) using RT-PCR. At least one subtype of channels could be detected in each tumor. High-grade gliomas expressed fewer sodium channel subtypes and these at weaker levels than low-grade tumors. Expression of Na(v)1.6, the most abundant isoform in the CNS, was almost absent in the gliomas except the pilocytic variant. Our study gives clear evidence for a differential expression of sodium channel subtypes in gliomas and indicates a predominant expression of channels related to malignancy grades.
Schrey
Michael
M
Institute of Pathology (Neuropathology), Friedrich Schiller University-Jena, Bachstrasse 18, D-07740 Jena, Germany.
Codina
Carolina
C
Kraft
Robert
R
Beetz
Christian
C
Kalff
Rolf
R
Wölfl
Stefan
S
Patt
Stephan
S
D000368:Aged; D001932:Brain Neoplasms / Q000503:physiopathology*; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000503:physiopathology*; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D015222:Sodium Channels / Q000235:genetics*
D012333:RNA, Messenger; D015222:Sodium Channels