title: Ovarian response, embryo production and hormonal profile in superovulated goats treated with insulin.
abstract: The influence of insulin on ovarian response and embryo production was investigated in 30 mixed breed goats, divided randomly into three equal (n=10) groups. Goats in Group 1 (control) were superovulated using 20 IU FSH i.m. in six divided descending doses, i.e. 4/4, 3/3 and 3/3 IU at 12 h interval for three consecutive days and were not given insulin treatment. Goats in Group 2 (insulin pretreatment) were pretreated with long acting purified bovine insulin 0.2 IU/kg body weight per day s.c. on Days 7, 8 and 9 of the estrous cycle prior to initiation of superovulatory treatment as in Group 1. Animals in Group 3 (insulin cotreatment) were treated as in Group I, but in addition received long acting purified bovine insulin 0.2 IU/kg body weight per day s.c. as a cotreatment along with the first, third and fifth FSH treatments on three consecutive days. Total ovarian response (corpus luteum and unovulated large follicle (UOLF)) was significantly (P<0.05) higher in insulin pretreatment (17.90+/-3.08) than in the cotreatment (11.50+/-2.34) and control (11.90+/-1.87) groups. The number of UOLF was significantly higher (P<0.05) in the insulin pretreatment (10.2+/-1.67) than the cotreatment (4.9+/-1.14) and control (3.6+/-1.09) groups. The mean transferable quality of embryos did not differ significantly among treatments. Progesterone concentration on the day of PGF(2)alpha treatment was not different (P>0.05) between the insulin treatment groups (5.28+/-0.79; 5.30+/-0.66 ng/ml). Estradiol-17beta concentration was significantly (P<0.05) higher on the day of PGF(2)alpha treatment in both the insulin treatment groups (36.67+/-6.40; 34.33+/-4.33 pg/ml) as compared to the control group (20.00+/-2.73 pg/ml). There is ample evidence to indicate beneficial effect of insulin on folliculogenesis and steroidogenesis in superovulated goats.
authors.affiliation: Division of Animal Physiology, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560030, India. sraju@nainpbng.kar.nic.in
authors.forename: S
authors.initials: S
authors.lastname: Selvaraju
authors.forename: S K
authors.initials: SK
authors.lastname: Agarwal
authors.forename: S D
authors.initials: SD
authors.lastname: Karche
authors.forename: A C
authors.initials: AC
authors.lastname: Majumdar
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D005314:Embryonic and Fetal Development / Q000187:drug effects / Q000502:physiology; D004958:Estradiol / Q000097:blood; D004971:Estrus / Q000502:physiology; D005260:Female; D006041:Goats / Q000196:embryology / Q000502:physiology*; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D011897:Random Allocation; D013480:Superovulation / Q000502:physiology*
chemical_list: D001786:Blood Glucose; D007328:Insulin; D011374:Progesterone; D004958:Estradiol