[Establishment and primary study of an assay for the detection of EB virus specific cytotoxic T lymphocytes].
In our study, a non-radioactive cytotoxicity assay by using a Balb/C (H-2d) mice, P815(H-2d) cells and lactate dehydrogenase (LDH) system was established, in order to detect EB virus specific cytotoxic T lymphocytes(CTLs). In this study, we detected both the changes of primary CTLs and secondary stimulated CTLs. Spleen CTL activity assay showed that the levels of primary and secondary CTLs in recombinant EBV-LMP1 vaccinia virus group were more higher than that in control group, at the same time, obvious enhancement of EBV-LMP1 specific CTL activity was observed in experimental group immunized by Baculovirus system expressing EBV-LMP1 protein when compared with the normal mice group. This work proved that the LDH assay can be used to detect cytotoxic T lymphocytes.
Liu
H
H
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052.
Zhou
L
L
Zeng
Y
Y
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics*; D007770:L-Lactate Dehydrogenase; D051379:Mice; D008807:Mice, Inbred BALB C; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured; D014616:Vaccinia virus / Q000235:genetics*; D014763:Viral Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
D004274:DNA, Recombinant; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins; D007770:L-Lactate Dehydrogenase