Evaluation of humoral response to tumor antigens using recombinant expression-based serological mini-arrays (SMARTA).
Screening of expression cDNA libraries derived from human neoplasms with autologous sera (SEREX) is an established method for defining antigens immunogenic in individual cancer patients. Although the majority of SEREX-derived cDNA clones encode autoantigens, some of them represent shared cancer antigens with cancer-related serological profiles. Routine evaluation of multiple SEREX-derived clones in serological assays using panels of allogeneic sera from cancer patients is an important step towards defining disease parameters of diagnostic and prognostic significance. Here we show how the seroreactivity of multiple SEREX-derived antigens can be simultaneously evaluated using a rapid semi-quantitative protocol of allogeneic screening, which we call SMARTA (serological mini-arrays of recombinant tumor antigens).
Lagarkova
Maria A
MA
Department of Molecular Immunology, Belozersky Institute of Physico-Chemical Biology, Center for Molecular Medicine, Moscow State University, Vorobjovy Gory, 119899, Moscow, Russia.
Koroleva
Ekaterina P
EP
Kuprash
Dmitry V
DV
Boitchenko
Veronica E
VE
Kashkarova
Ulyana A
UA
Nedospasov
Sergei A
SA
Shebzukhov
Yuriy V
YV
D000912:Antibodies, Neoplasm; D000917:Antibody Formation / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000235:genetics / Q000276:immunology*; D001323:Autoantibodies; D003001:Cloning, Molecular; D018076:DNA, Complementary; D020869:Gene Expression Profiling; D015723:Gene Library; D006801:Humans; D011994:Recombinant Proteins / Q000276:immunology; D012698:Serologic Tests / Q000379:methods*
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D001323:Autoantibodies; D018076:DNA, Complementary; D011994:Recombinant Proteins