A supercritical fluid-based coating technology. 3: preparation and characterization of bovine serum albumin particles coated with lipids.
Solvent-free microparticles, loaded with bovine serum albumin as a model protein, were produced using a novel supercritical (SC) fluid-based coating technology. Coating material consists either of trimyristin (Dynasan 114) or of Gelucire 50-02. Microparticles obtained were characterized as regards their morphology, protein content and in vitro release profile. A discontinuous coating made of micro-needles of trimyristin led to an initial burst release of approximately 70% in 30 min. However, a prolonged release of the BSA could be achieved in a phosphate buffer solution at 37 degrees C over a 24 h period from particles coated with Gelucire 50-02. Furthermore, it was shown that BSA does not undergo any degradation after SC CO(2) treatment under the conditions used in the coating process.
Ribeiro Dos Santos
I
I
Unité de Vectorization Particulaire INSERM - ERIT M 0104, 10, rue A Boquel, 49100 Angers cedex, France.
Richard
J
J
Thies
C
C
Pech
B
B
Benoit
J-P
JP
D002214:Capsules; D002245:Carbon Dioxide / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D004339:Drug Compounding / Q000379:methods; D016503:Drug Delivery Systems; D005223:Fats / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009821:Oils / Q000737:chemistry; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000008:administration & dosage* / Q000737:chemistry; D012995:Solubility; D013499:Surface Properties; D013678:Technology, Pharmaceutical; D013696:Temperature
D002214:Capsules; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005223:Fats; D008055:Lipids; D009821:Oils; C071183:Gelucire 50-02; D002245:Carbon Dioxide; D012710:Serum Albumin, Bovine