title: Rapid microsatellite analysis using discontinuous polyacrylamide gel electrophoresis.
abstract: A method for screening large numbers of samples for microsatellites using discontinuous, non-denaturing polyacrylamide gels and rapid fluorescent gel staining is described. Disc electrophoresis on slab gels provides high-resolution of PCR products. It is useful for collecting population data once microsatellite loci have been characterized.
authors.affiliation: Pacific Forestry Centre, Canadian Forest Service, 506 West Burnside Road, Victoria, BC V8Z 1M5, Canada. ewhite@pfc.forestry.ca
authors.forename: E
authors.initials: E
authors.lastname: White
authors.forename: R
authors.initials: R
authors.lastname: Sahota
authors.forename: S
authors.initials: S
authors.lastname: Edes
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers