Myosin light chain kinase inhibitors induce retraction of mature oligodendrocyte processes.
Mature oligodendrocytes emit numerous myelinating processes. Force generating molecules are required for process outgrowth and spreading. We have analyzed the effect of the myosin II light chain kinase inhibitors ML-7 and ML-9 in cultured oligodendrocytes. Both drugs affect oligodendrocyte cell shape, provoking a retraction of high order processes. Our results suggest that the adhesion of the myelinating processes to the substrate depends on MLC phosphorylation, thus likely implicating myosin IIA.
Thomas
María G
MG
IIB Fundación Campomar, IIB FCEyN, University of Buenos Aires and IIBBA-CONICET, Buenos Aires, Argentina.
Santa Coloma
Tomás A
TA
Correale
Jorge
J
Boccacci
Graciela L
GL
D000818:Animals; D001381:Azepines / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D009219:Myosin-Light-Chain Kinase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009281:Naphthalenes / Q000494:pharmacology*; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar
D001381:Azepines; D004791:Enzyme Inhibitors; D009281:Naphthalenes; C056218:ML 9; C070571:ML 7; D009219:Myosin-Light-Chain Kinase