Live cell imaging: approaches for studying protein dynamics in living cells.
In the last decade, the long-standing biologist's dream of seeing the molecular events within the living cell came true. This technological achievement is largely due to the development of fluorescence microscopy technologies and the advent of green fluorescent protein as a fluorescent probe. Such imaging technologies allowed us to determine the subcellular localization, mobility and transport pathways of specific proteins and even visualize protein-protein interactions of single molecules in living cells. Direct observation of such molecular dynamics can provide important information about cellular events that cannot be obtained by other methods. Thus, imaging of protein dynamics in living cells becomes an important tool for cell biology to study molecular and cellular functions. In this special issue of review articles, we review various imaging technologies of microscope hardware and fluorescent probes useful for cell biologists, with a focus on recent development of live cell imaging.
Haraguchi
Tokuko
T
CREST Research Project of the Japan Science and Technology Corporation, Kansai Advanced Research Center, Kobe, Japan. tokuko@crl.go.jp
D000818:Animals; D005057:Eukaryotic Cells / Q000166:cytology* / Q000378:metabolism; D036681:Fluorescence Recovery After Photobleaching; D031541:Fluorescence Resonance Energy Transfer; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D011506:Proteins / Q000378:metabolism*
D008164:Luminescent Proteins; D011506:Proteins; D049452:Green Fluorescent Proteins