Immobilization stress-induced increment of lactate metabolism in the basolateral amygdaloid nucleus is attenuated by diazepam in the rat.
Using in vivo microdialysis technique, extracellular lactate levels were measured in the basolateral amygdaloid nucleus of the rat under immobilization stress. Immobilization stress (40 min) led to a tetrodotoxin-reversible increase in dialysate lactate levels. Diazepam (1.0 mg/kg, i.p.) reduced the ability of immobilization stress to increase lactate levels. Furthermore, the attenuation of the immobilization stress-induced increase of lactate levels by diazepam was antagonized by pretreatment with flumazenil (15 mg/kg, i.p.), a selective antagonist at benzodiazepine receptors. These findings suggest that immobilization stress increases lactate levels in rat basolateral amygdaloid nuclei, which is attenuated by stimulation of benzodiazepine receptors.
Uehara
Takashi
T
Department of Neuropsychiatry, Faculty of Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, 930-0194, Toyama, Japan. uehara@ms.toyama-mpu.ac.jp
Kurata
Kouichi
K
Sumiyoshi
Tomiki
T
Kurachi
Masayoshi
M
D000679:Amygdala / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D003975:Diazepam / Q000494:pharmacology* / Q000627:therapeutic use; D007103:Immobilization; D019344:Lactic Acid / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000188:drug therapy / Q000378:metabolism*
D019344:Lactic Acid; D003975:Diazepam