Ethanol differently affects stress protein and HERG K+ channel expression in SH-SY5Y cells.
Ethanol is known to be neurotoxic. Protective mechanisms, however, are activated upon ethanol induction of the glucose-regulated stress proteins (GRPs), GRP78 and GRP94. These endoplasmic reticulum-residing chaperones are known to be involved in channel subunit assembly. The GRP and human-ether-à-gogo-related gene (HERG) K(+)-channel expression were monitored in short- and long-term ethanol incubation experiments using the human neuroblastoma cell line SH-SY5Y. mRNA of the stress proteins and protein levels of the GRPs and HERG were determined using Northern and Western blot methods. Short-term ethanol incubation caused a transient increase of GRP transcripts. Protein levels of GRP94 decreased in chronic experiments, whereas GRP78 did not change. HERG followed the same kinetics as GRP94 with a constant down-regulation. The coordinate down-regulation of GRP94 and HERG implies the specific involvement of the endoplasmic reticulum chaperone GRP94 and HERG, but not GRP78, in a process of cell adaptation.
Mühlbauer
Eckhard
E
Institute of Anatomy and Cell Biology, Martin-Luther-University of Halle-Wittenberg/Saxon Academy of Sciences, Leipzig, Grosse Steinstrasse 52, 06097, Halle, Germany. ekhard.muehlbauer@medizin.uni-halle.de
Rommelspacher
Hans
H
D027682:Cation Transport Proteins; D004268:DNA-Binding Proteins; D000072237:ERG1 Potassium Channel; D000091342:Endoplasmic Reticulum Chaperone BiP; D000431:Ethanol / Q000494:pharmacology*; D051638:Ether-A-Go-Go Potassium Channels; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism*; D015221:Potassium Channels / Q000096:biosynthesis*; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger / Q000096:biosynthesis; D015534:Trans-Activators; D000071230:Transcriptional Regulator ERG; D014407:Tumor Cells, Cultured
D027682:Cation Transport Proteins; D004268:DNA-Binding Proteins; C072312:ERG protein, human; D000072237:ERG1 Potassium Channel; D000091342:Endoplasmic Reticulum Chaperone BiP; D051638:Ether-A-Go-Go Potassium Channels; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; C000606913:KCNH2 protein, human; C092509:KCNH6 protein, human; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger; D015534:Trans-Activators; D000071230:Transcriptional Regulator ERG; D000431:Ethanol