title: BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
abstract: The BayGenomics gene-trap resource (http://baygenomics.ucsf.edu) provides researchers with access to thousands of mouse embryonic stem (ES) cell lines harboring characterized insertional mutations in both known and novel genes. Each cell line contains an insertional mutation in a specific gene. The identity of the gene that has been interrupted can be determined from a DNA sequence tag. Approximately 75% of our cell lines contain insertional mutations in known mouse genes or genes that share strong sequence similarities with genes that have been identified in other organisms. These cell lines readily transmit the mutation to the germline of mice and many mutant lines of mice have already been generated from this resource. BayGenomics provides facile access to our entire database, including sequence tags for each mutant ES cell line, through the World Wide Web. Investigators can browse our resource, search for specific entries, download any portion of our database and BLAST sequences of interest against our entire set of cell line sequence tags. They can then obtain the mutant ES cell line for the purpose of generating knockout mice.
authors.affiliation: Department of Pharmaceutical Chemistry, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA.
authors.forename: Doug
authors.initials: D
authors.lastname: Stryke
authors.forename: Michiko
authors.initials: M
authors.lastname: Kawamoto
authors.forename: Conrad C
authors.initials: CC
authors.lastname: Huang
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Johns
authors.forename: Leslie A
authors.initials: LA
authors.lastname: King
authors.forename: Courtney A
authors.initials: CA
authors.lastname: Harper
authors.forename: Elaine C
authors.initials: EC
authors.lastname: Meng
authors.forename: Roy E
authors.initials: RE
authors.lastname: Lee
authors.forename: Alice
authors.initials: A
authors.lastname: Yee
authors.forename: Larry
authors.initials: L
authors.lastname: L'Italien
authors.forename: Pao-Tien
authors.initials: PT
authors.lastname: Chuang
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Young
authors.forename: William C
authors.initials: WC
authors.lastname: Skarnes
authors.forename: Patricia C
authors.initials: PC
authors.lastname: Babbitt
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Ferrin
mesh_terms: D000818:Animals; D002460:Cell Line; D030561:Databases, Nucleic Acid; D004622:Embryo, Mammalian / Q000166:cytology*; D023281:Genomics; D020407:Internet; D051379:Mice / Q000235:genetics*; D018345:Mice, Knockout; D016254:Mutagenesis, Insertional; D009154:Mutation; D013234:Stem Cells / Q000166:cytology*; D014584:User-Computer Interface