Lymphocytes expressing alpha1beta1 integrin (very late antigen-1) in peripheral blood of patients with arthritis are a subset of CD45RO(+) T-cells primed for rapid adhesion to collagen IV.
We report that very late antigen-1 (VLA-1(+)) CD3(+)CD45RO(+) T-cells are selectively segregated from VLA-1(-) peripheral blood (PB) mononuclear cells (MC), in which CD3(+) T-cells are evenly CD45RO(+) and CD45RO(-), when PBMC are stained with a monoclonal antibody (mAb) to VLA-1 and passaged on immunomagnetic columns. In contrast, both VLA-1(+) and VLA-1(-) MC isolated from synovial fluid (SF) are mainly CD45RO(+)CD3(+) T-cells. VLA-1(+) MC formed 13 +/- 5.3% of MC eluting from columns loaded with PBMC of patients with seropositive rheumatoid arthritis (n = 6) and 2.3 +/- 1.6% of patients (n = 4) with other arthritides (P < 0.022). Importantly, only the VLA-1(+) MC from PB and SF adhered to collagen IV upon triggering with phorbol 12-myristate 13-acetate. Moreover, adhesion and migration on collagen IV were preferentially maintained in lines cultured from VLA-1(+) T-cells, and both were inhibited by mAb to the VLA-1 alpha1 I domain. These results suggest that VLA-1(+) CD45RO(+) T-cells in patients with arthritis could play a role in both systemic and local inflammation by rapidly adhering to collagen IV.
Bank
Ilan
I
Department of Medicine F, Chaim Sheba Medical Center and Tel Aviv University, Tel Hashomer, 52621, Israel.
Koltakov
Alexander
A
Goldstein
Itamar
I
Chess
Leonard
L
D000911:Antibodies, Monoclonal; D001168:Arthritis / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology; D002448:Cell Adhesion; D002465:Cell Movement; D024141:Collagen Type IV / Q000378:metabolism*; D006801:Humans; D039222:Integrin alpha1beta1 / Q000378:metabolism*; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D013582:Synovial Fluid / Q000166:cytology; D016176:T-Lymphocyte Subsets / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D024141:Collagen Type IV; D039222:Integrin alpha1beta1; D017493:Leukocyte Common Antigens