title: Restriction enzyme recognition sequence search program.
abstract: A critical and difficult part of characterizing restriction enzymes and methylases is the identification of recognition sequences. To simplify this process, we have developed a plasmid transformation method along with a computer program named RM search that determines the exact recognition sequences for given restriction and modification systems.
authors.affiliation: University of California, Riverside, Riverside, CA, USA.
authors.forename: Kyle P
authors.initials: KP
authors.lastname: Ellrott
authors.forename: Julie K A
authors.initials: JK
authors.lastname: Kasarjian
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Junichi
authors.initials: J
authors.lastname: Ryu
mesh_terms: D000465:Algorithms; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D016384:Consensus Sequence; D015254:DNA Modification Methylases / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000737:chemistry / Q000378:metabolism; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D012984:Software; D013379:Substrate Specificity
chemical_list: D004279:DNA, Viral; D015254:DNA Modification Methylases; D004262:DNA Restriction Enzymes