title: Dendritic cells are decreased in blood and accumulated in granuloma in tuberculosis.
abstract: Immunity against tuberculosis consists of innate and adaptive immune responses. In this study, we investigated the dynamics of dendritic cells (DC), which are known to elicit a variety of immune responses, in patients with tuberculosis. CD11c(+) peripheral blood DC were decreased in patients with tuberculosis. Immunohistochemical analyses demonstrated that a number of fascin(+), CD11c(+), HLA-DR(+) DC were infiltrating the lymphocyte areas of the tuberculous granulomas (tubercles). Immunohistochemical analyses also demonstrated that interferon-gamma-producing Th1 cells were increased in the tubercles of the patients, indicating the presence of Th1 polarization at least in the context of inflammatory tissues. In vitro coculture of autologous naive T cells with CD11c(+) or CD11c(-) DC pretreated with Bacillus Calmette Guérin augmented the production of Th1 cells. These findings suggested that the trafficking of DC from the peripheral blood into the tubercles causes a dominant Th1 balance and thus plays an essential role in the immunity against tuberculosis.
authors.affiliation: First Department of Internal Medicine, Kansai Medical University, 10-15 Fumizonocho, Moriguchi, Osaka, 570-8506, Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Uehira
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Amakawa
authors.forename: Tomoki
authors.initials: T
authors.lastname: Ito
authors.forename: Kenichirou
authors.initials: K
authors.lastname: Tajima
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Naitoh
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Ozaki
authors.forename: Toshiki
authors.initials: T
authors.lastname: Shimizu
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Uemura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kitajima
authors.forename: Seibun
authors.initials: S
authors.lastname: Yonezu
authors.forename: Shirou
authors.initials: S
authors.lastname: Fukuhara
mesh_terms: D039521:CD11c Antigen / Q000032:analysis; D002454:Cell Differentiation; D002465:Cell Movement; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006099:Granuloma / Q000276:immunology*; D006801:Humans; D008297:Male; D009163:Mycobacterium bovis / Q000276:immunology; D013601:T-Lymphocytes; D018417:Th1 Cells / Q000276:immunology*; D014376:Tuberculosis / Q000097:blood / Q000276:immunology* / Q000473:pathology
chemical_list: D039521:CD11c Antigen