title: Recombinagenic effect of cryptolepine in uvsH+//uvsH+ and uvsH//uvsH diploid strains of Aspergillus nidulans.
abstract: Recombinagenic potential of the alkaloid cryptolepine was evaluated in two diploid strains of Aspergillus nidulans--a wild type strain (uvsH+//uvsH+) and a DNA-repair-deficient one (uvsH//uvsH). Treatment of both strains with cryptolepine failed to alter colony growth in culture; its recombinagenic potential was determined by the homozygotization index (in which events of mitotic exchange may cause expression of genes previously masked by the dominant allele). Mitotic crossing-overs were induced by 7 and 14 mg/L doses of cryptolepine in both diploid A. nidulans strains.
authors.affiliation: Department of Cell Biology and Genetics, State University of Maringá, 87 020-900 Maringá, Brazil.
authors.forename: S J R
authors.initials: SJ
authors.lastname: Chiuchetta
authors.forename: M A A
authors.initials: MA
authors.lastname: Castro-Prado
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D001233:Aspergillus nidulans / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D003434:Crossing Over, Genetic; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004171:Diploidy; D005656:Fungal Proteins; D026121:Indole Alkaloids; D007211:Indoles / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008938:Mitosis / Q000187:drug effects; D011804:Quinolines / Q000494:pharmacology*; D011995:Recombination, Genetic; D059005:Topoisomerase II Inhibitors
chemical_list: D000470:Alkaloids; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D026121:Indole Alkaloids; D007211:Indoles; D011804:Quinolines; D059005:Topoisomerase II Inhibitors; C095234:UvsH protein, Aspergillus nidulans; C024015:cryptolepine