[Antisense oligodeoxynucleotide targeted at the pre-s region of DHBV could inhibit virus replication in vivo].
To study the antiviral effect of antisense oligodeoxynucleotide (AS-ODN), AS-ODN phosphotioates targeted to DHBV Pre-S region (951-968nt) (5'-TATCTCCTCCATTGTTTG-3') was synthesized. Two day-old ducklings were infected i.p. with 150 microliters of DHBV (3 x 10(8) copies/ml DHBV). After 12 days, 6 DHBsAg and DHBV DNA positive ducks were divided into two groups. In treated group, each duck was injected i.v. AS-ODN 20 micrograms/gram weight/day for 10 dyas successively, while in the control group, each duck received the same volume of normal saline for 10 days. Sera from ducks prior to treatment and treated after 6, 10 days were collected, and after 10 days ducks were sacrificed and livers were examined. DHBsAg was significantly decreased in all three treated ducks after 10 days of AS-ODN treatment. One of these ducks showed decreased DHBsAg even 6 days after treatment. Two out of three treated ducks showed decreased serum DHBV DNA, while one remained unchanged. In contrast, none of the control group showed decreased DHBsAg and DHBV DNA did not change. By Southern blot, DHBV DNA in the liver significantly decreased in 2/3 of the treated ducks and decreased in the other duck. In all control ducks, DHBV DNA remained strongly positive. Data indicated that AS-ODN targeting at Pre-S region could partly inhibit the replication of DHBV.
He
L
L
Department of Molecular Virology, Shanghai Medical University, Shanghai 200032.
Wu
X
X
Chen
C
C
D000818:Animals; D004372:Ducks; D015316:Genetic Therapy; D018347:Hepadnaviridae Infections / Q000821:virology; D015611:Hepatitis B Virus, Duck / Q000235:genetics*; D006524:Hepatitis, Viral, Animal / Q000821:virology; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D014759:Viral Envelope Proteins / Q000235:genetics*; D014779:Virus Replication / Q000187:drug effects*
D020319:Oligodeoxyribonucleotides, Antisense; C077433:Pre-S protein, Duck hepatitis B virus; D014759:Viral Envelope Proteins