title: Calreticulin-like molecule in trophozoites of Entamoeba histolytica HM1:IMSS (Swissprot: accession P83003).
abstract: In this work, we report the partial sequence of a 51 kDa protein of Entamoeba histolytica that is highly immunogenic in humans. Partial sequencing of the N-terminal end showed that 18 of the first 20 amino acid residues of the protein were identified uniquely, indicating that the final product was a homogeneous protein preparation. The N-terminal sequence that was found was: KVYFEETFENGWKXIWSKW. Comparing the 19-amino acid sequence of the protein in automated databases shows significant similarity with amino acid sequences of the calreticulin-like protein of spinach leaves (77%) and of the calreticulin precursor of Dictyostelium discoideum (60%).
authors.affiliation: Depto. de Medicina Experimental, Facultad de Medicine, UNAM, México, DF, México.
authors.forename: Enrique
authors.initials: E
authors.lastname: González
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Rico
authors.forename: Guillermo
authors.initials: G
authors.lastname: Mendoza
authors.forename: Fernando
authors.initials: F
authors.lastname: Ramos
authors.forename: Gabriela
authors.initials: G
authors.lastname: García
authors.forename: Patricia
authors.initials: P
authors.lastname: Morán
authors.forename: Alicia
authors.initials: A
authors.lastname: Valadez
authors.forename: Emma I
authors.initials: EI
authors.lastname: Melendro
authors.forename: Cecilia
authors.initials: C
authors.lastname: Ximénez
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D015153:Blotting, Western; D037282:Calreticulin / Q000737:chemistry* / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004748:Entamoeba histolytica / Q000254:growth & development* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry* / Q000276:immunology*; D020539:Sequence Analysis, Protein
chemical_list: C470490:51 kDa protein, Entamoeba histolytica; D000913:Antibodies, Protozoan; D037282:Calreticulin; D015800:Protozoan Proteins