title: [Genetic typing of Mycobacterium tuberculosis strains by spoligotyping and genome fingerprinting techniques].
abstract: A total of 122 M. tuberculosis clinical drug-resistant strains isolated in Central Russia were studied by spoligotyping and genome fingerprinting techniques. According to spoligotyping results 77% of M. tuberculosis strains were distributed to 13 oligotypes, while 23% of these strains were found to form unique patterns. Most of them belonged to the families Beijing and Haarlem (43.4% and 13.9% respectively). The patterns of the strains of oligotype 12 (7F-7F-7E-0E-78-3E) were identical to those of the strains isolated in Brazil, France and the Netherlands. The strains of the spoligotype 22 (7F-1E-7F-7F-07-3E) had the patterns identical to those of the strains of group S13, also isolated in Brazil. According to genome fingerprinting 31.4% of the strains were found to belong to clusters with the similarity coefficient equal to 1. The strains belonging to genotypes W and A1 were found to prevail in the analyzed group.
authors.affiliation: State Research Center of Applied Microbiology, Obolensk, Moscow Region, Russia.
authors.forename: I G
authors.initials: IG
authors.lastname: Shemiakin
authors.forename: V N
authors.initials: VN
authors.lastname: Stepanshina
authors.forename: O V
authors.initials: OV
authors.lastname: Korobova
authors.forename: V A
authors.initials: VA
authors.lastname: Anisimova
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Ivanov
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Lipin
authors.forename: A  A
authors.initials: A
authors.lastname: Lazarev
authors.forename: A I
authors.initials: AI
authors.lastname: Skaldina
authors.forename: L A
authors.initials: LA
authors.lastname: Chernavskaia
authors.forename: T I
authors.initials: TI
authors.lastname: Tarasova
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D005838:Genotype; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D009841:Oligonucleotides / Q000032:analysis; D012150:Polymorphism, Restriction Fragment Length; D021521:Ribotyping / Q000379:methods*; D012426:Russia; D013045:Species Specificity; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology*; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D004269:DNA, Bacterial; D009841:Oligonucleotides