[Protective effect of bajitian oligosaccharide on PC12 cells lesioned by corticosterone].
OBJECTIVE
To study the possible mechanism of antidepressant effect of Bajitian oligosaccharide (MW-97).


RESULTS
After incubating with the PC12 cells in the presence of corticosterone(2 x 10(-4) mol.L-1) for 48 h, it was found that MW-97 could protect PC12 cells from the lesion done by corticosterone in a concentration-dependent manner. Furthermore, using RT-PCR mediated cross-species partial cDNA cloning, it was found that MW-97 and desipramine(DIM) increased NGF, BDNF mRNA in the frontal cortex after chronic administration for 21 days, meanwhile, BDNF mRNA in hippocampus was also increased.


CONCLUSIONS
The cytoprotective effect of MW-97 consists in the possible mechanism of its antidepressant action, while the increasing expression of neurotrophic factors (NGF, BDNF mRNA) may be contributes to the cytoprotective effect.
Li
Y F
YF
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
Yang
M
M
Zhao
Y M
YM
Luo
Z P
ZP
D000818:Animals; D000928:Antidepressive Agents / Q000302:isolation & purification / Q000494:pharmacology; D001921:Brain / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D020932:Nerve Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology; D009844:Oligosaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D016716:PC12 Cells / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D019666:Rubiaceae / Q000737:chemistry*
D000928:Antidepressive Agents; D019208:Brain-Derived Neurotrophic Factor; D018696:Neuroprotective Agents; D009844:Oligosaccharides; D012333:RNA, Messenger; D020932:Nerve Growth Factor