[The effect of cardiopulmonary bypass on expression of myocardial nitric oxide synthase in patients for valve replacement].
The objective of this study was to assess the effect of cardiopulmonary bypass (CPB) on expression of nitric oxide synthase (NOS) in patients for valve replacement. Ten adult patients were included in this study. CPB was initiated with a bubble oxygenator, aorta was clamped, and cold crystalliod cardioplegia was delivered into the aortic root. The myocardium of atrial appendage was obtained pre-CPB and 45 minutes post-CPB. The expression of three kinds of NOS isoenzymes was assessed by immunohistochemistry and microcomputerized image analysis. The results demonstrate that there are expression of nNOS (nervous NOS), iNOS (inducible NOS) and eNOS (endothelial NOS) in the atrial myocardium of patients for valve replacement. Compared with the expressions of NOS pre-CPB, the post-CPB expression of nNOS increased and that of eNOS decreased (P < 0.05), but no significant change in expression of iNOS was noted(P > 0.5). We belive that the decreased expression of eNOS may be related with the deficiency of nitric oxide release and may be responsible for tissue damage during CPB.
Zhou
X
X
Department of Histology, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
Xao
X
X
Zhang
W
W
Yang
H
H
Bao
T
T
D000328:Adult; D002315:Cardiopulmonary Bypass; D019918:Heart Valve Prosthesis Implantation; D006801:Humans; D009206:Myocardium / Q000201:enzymology*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D011184:Postoperative Period
C496320:NOS1 protein, human; C496319:NOS2 protein, human; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III