[The effects of sera from patients with paroxysmal nocturnal hematoglobinuria on the growth of CD34(+) cells].
To investigate the effects of sera on the growth of single CD34(+) cells from patients with paroxysmal nocturnal hematoglobinuria (PNH), sera from both PNH patients and normal individuals were added separately to the single cell culture system and semi-solid colony formation system. The growth of the normal CD34(+) and PNH CD34(+) CD59(+) and CD34(+) CD59(-) cells was evaluated. No growth difference was found for growth of the normal CD34(+) and PNH CD34(+) CD59(+) cells when PNH or normal sera were added to the culture media in either single cell culture or colony formation culture. While no difference was detected for single PNH CD34(+) CD59(-) cells growth when PNH or normal sera were added, more colonies were observed in semi-solid culture with PNH sera. A conclusion was reached that compared with those from normal controls, the sera from PNH patients had no significant influence on single hematopoietic stem/progenitor cells derived from normal subjects and from PNH patients, but the PNH sera might promote the colony formation of the CD34(+) CD59(+) cells in semi-solid culture
Han
Bing
B
Department of Hematology, Chinese Academy of Medical Sciences, Peking Union Medical College, Peking Union Medical College Hospital, Beijing 100730, China.
Wu
Yong-Ji
YJ
Zhang
Zhi-Nan
ZN
D018952:Antigens, CD34 / Q000032:analysis; D001798:Blood Proteins / Q000494:pharmacology*; D018957:CD59 Antigens / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000473:pathology; D006457:Hemoglobinuria, Paroxysmal / Q000276:immunology / Q000473:pathology*; D006801:Humans
D018952:Antigens, CD34; D001798:Blood Proteins; D018957:CD59 Antigens