[The effect of sodium azide on the thermotolerance of the yeast Saccharomyces cerevisiae and Candida albicans].
The addition of sodium azide (a mitochondrial inhibitor) at a concentration of 0.15 mM to glucosegrown Saccharomyces cerevisiae or Candida albicans cells before exposing them to heat shock increased cell survival. At higher concentrations of azide, its protective effect on glucose-grown cells decreased. Furthermore, azide, even at low concentrations, diminished the thermotolerance of galactose-grown yeast cells. It is suggested that azide exerts a protective effect on the thermotolerance of yeast cells when their energy requirements are met by the fermentation of glucose. However, when cells obtain energy through respiratory metabolism, the azide inhibition of mitochondria enhances damage inflicted on the cells by heat shock.
Rikhvanov
E G
EG
Siberian Institute of Plant Physiology and Biochemistry, Siberian Division, Russian Academy of Sciences, ul. Lermontova 132, Irkutsk, 664033 Russia.
Varakina
N N
NN
Rusaleva
T M
TM
Rachenko
E I
EI
Voĭnikov
V K
VK
D002176:Candida albicans / Q000254:growth & development / Q000502:physiology*; D003470:Culture Media; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005690:Galactose; D005947:Glucose; D018869:Heat-Shock Response / Q000187:drug effects; D006358:Hot Temperature; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000502:physiology*; D019810:Sodium Azide / Q000494:pharmacology*
D003470:Culture Media; D004791:Enzyme Inhibitors; D019810:Sodium Azide; D005947:Glucose; D005690:Galactose