Immunocytochemical description of five bipolar cell types of the mouse retina.
With the ever-growing number of transgenic mice being used in vision research, a precise knowledge of the cellular organization of the mouse retina is required. As with the cat, rabbit, rat, and primate retinae, as many as 10 cone bipolar types and one rod bipolar type can be expected to exist in the mouse retina; however, they still have to be defined. In the current study, several immunocytochemical markers were applied to sections of mouse retina, and the labeling of bipolar cells was studied using confocal microscopy and electron microscopy. By using antibodies against the neurokinin-3 receptor NK3R; the plasma membrane calcium ATPase1 (PMCA1); and the calcium (Ca)-binding proteins CaB1, CaB5, caldendrin, and recoverin, three different OFF-cone bipolar cells could be identified. One type of ON-cone bipolar cell was identified through its immunoreactivity for CaB5 and PMCA1. Rod bipolar cells, comparable in morphology to those of other mammalian retinae, expressed protein kinase Calpha and CaB5. It was also shown that putative OFF-cone bipolar cells receive light signals through flat contacts at the cone pedicle base, whereas ON-cone bipolar signaling involves invaginating contacts. The distribution of the kainate receptor subunit GluR5 was studied by confocal and electron microscopy. GluR5 was expressed at flat bipolar cell contacts; however, it appears to be involved with only certain types of OFF-cone bipolar cells. This suggests that different bipolar cell types receive their light signals through different sets of glutamate receptors.
Haverkamp
Silke
S
Max-Planck-Institut für Hirnforschung, D-60528 Frankfurt/Main, Germany.
Ghosh
Krishna K
KK
Hirano
Arlene A
AA
Wässle
Heinz
H
D000818:Animals; D000918:Antibody Specificity; D015415:Biomarkers / Q000032:analysis; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000096:biosynthesis; D000252:Calcium-Transporting ATPases / Q000032:analysis / Q000096:biosynthesis; D027682:Cation Transport Proteins; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D053499:Plasma Membrane Calcium-Transporting ATPases; D011493:Protein Kinase C / Q000032:analysis / Q000096:biosynthesis; D051571:Protein Kinase C-alpha; D018092:Kainic Acid Receptors / Q000032:analysis / Q000096:biosynthesis; D018042:Receptors, Neurokinin-3 / Q000032:analysis / Q000096:biosynthesis; D012160:Retina / Q000166:cytology*; D017949:Retinal Cone Photoreceptor Cells / Q000166:cytology* / Q000378:metabolism; D017948:Retinal Rod Photoreceptor Cells / Q000166:cytology* / Q000378:metabolism
D015415:Biomarkers; D002135:Calcium-Binding Proteins; D000252:Calcium-Transporting ATPases; D027682:Cation Transport Proteins; D053499:Plasma Membrane Calcium-Transporting ATPases; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D018092:Kainic Acid Receptors; D018042:Receptors, Neurokinin-3; C496630:Prkca protein, mouse; C506954:Atp2b1 protein, mouse; C506955:Atp2b1 protein, rat