title: Chromosomal abnormalities in embryos derived from testicular sperm extraction.
abstract: OBJECTIVE
  To compare the rate of chromosome abnormalities in embryos obtained from karyotypically normal patients with nonobstructive azoospermia undergoing testicular sperm extraction (TESE) to those from patients undergoing intracytoplasmic sperm injection (ICSI) with ejaculated sperm.
  
  
  METHODS
  Retrospective analysis.
  
  
  METHODS
  IVF centers.
  
  
  METHODS
  Male partners had either nonobstructive zoospermia or oligospermia.
  
  
  METHODS
  Preimplantation genetic diagnosis. Chromosome enumeration was performed by fluorescence in situ hybridization (FISH). Embryos classified as abnormal were reanalyzed to study mosaicism.
  
  
  METHODS
  Chromosome abnormalities in embryos.
  
  
  RESULTS
  Embryos from ICSI cycles with ejaculated sperm (group 1) were 41.8% normal, 26.2% aneuploid, and 26.5% mosaic. In contrast, the embryos from ICSI cycles with TESE for nonobstructive azoospermia (group 2) were 22% normal, 17% aneuploid, and 53% mosaic. The difference in mosaicism rate between the two groups of embryos was highly significant.
  
  
  CONCLUSIONS
  The present study results indicate a high incidence of mosaicism in embryos derived from TESE in men with a severe deficit in spermatogenesis. Sperm derived from TESE for nonobstructive azoospermia may have a higher rate of compromised or immature centrosome structures leading to mosaicism in the embryo.
authors.affiliation: Infertility Center of St. Louis at St. Luke's Hospital, St. Louis, Missouri, USA.
authors.forename: Sherman
authors.initials: S
authors.lastname: Silber
authors.forename: Tomas
authors.initials: T
authors.lastname: Escudero
authors.forename: Kathleen
authors.initials: K
authors.lastname: Lenahan
authors.forename: Iman
authors.initials: I
authors.lastname: Abdelhadi
authors.forename: Zaid
authors.initials: Z
authors.lastname: Kilani
authors.forename: Santiago
authors.initials: S
authors.lastname: Munné
mesh_terms: D000782:Aneuploidy; D002869:Chromosome Aberrations; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009030:Mosaicism; D009845:Oligospermia / Q000628:therapy; D019836:Preimplantation Diagnosis; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa; D013737:Testis / Q000166:cytology*; D020858:Tissue and Organ Harvesting / Q000379:methods*