Novel methylotrophy genes of Methylobacterium extorquens AM1 identified by using transposon mutagenesis including a putative dihydromethanopterin reductase.
Ten novel methylotrophy genes of the facultative methylotroph Methylobacterium extorquens AM1 were identified from a transposon mutagenesis screen. One of these genes encodes a product having identity with dihydrofolate reductase (DHFR). This mutant has a C(1)-defective and methanol-sensitive phenotype that has previously only been observed for strains defective in tetrahydromethanopterin (H(4)MPT)-dependent formaldehyde oxidation. These results suggest that this gene, dmrA, may encode dihydromethanopterin reductase, an activity analogous to that of DHFR that is required for the final step of H(4)MPT biosynthesis.
Marx
Christopher J
CJ
Department of Microbiology, University of Washington, Seattle 98195-1750, USA.
O'Brien
Brooke N
BN
Breezee
Jennifer
J
Lidstrom
Mary E
ME
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004251:DNA Transposable Elements; D005816:Genetic Complementation Test; D000432:Methanol / Q000378:metabolism*; D020601:Methylobacterium extorquens / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D011622:Pterins / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D011622:Pterins; C036686:methanopterin; C042416:5,6,7,8-tetrahydromethanopterin; D010088:Oxidoreductases; D013762:Tetrahydrofolate Dehydrogenase; D000432:Methanol