Analysis of transcription of plant 7SL RNA gene variants in HeLa in vitro transcription system.
We have performed an analysis of transcription of hop 7SL RNA genes in heterologous human extract from HeLa cells. Several variants of the Hl7SL-1 gene with a truncated or mutated 5' non-transcribed part revealed a crucial importance of TATA box for transcription. The USE element was found important but dispensable for transcription. Transcription of mutants in the A-like box and experiments with hop 7SL RNA pseudogenes E44 and G32 revealed the importance of internal elements. The A-like box and possibly CG doublet at position +15/+16 described by Bredow et al. (1990a) are according to our results indispensable for in vitro transcription of plant 7SL RNA genes in human extract.
Vrba
L
L
Department of Molecular Genetics, Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Ceské Budejovice, Czech Republic.
Matousek
J
J
D001483:Base Sequence; D006367:HeLa Cells; D006801:Humans; D027582:Humulus / Q000235:genetics*; D008969:Molecular Sequence Data; D020733:RNA, Small Cytoplasmic / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D016415:Sequence Alignment; D018271:Signal Recognition Particle / Q000235:genetics*; D014158:Transcription, Genetic
C115990:7SL RNA; D020733:RNA, Small Cytoplasmic; D018271:Signal Recognition Particle