[Comparison of production and characters of antisera from rabbits and laying hens].
Rabbits and laying hens were immunized with the conjugate of aflatoxin B1 oxime and bovine serum albumin (AFB1 O-BSA). The production processes, titers and specificities of antisera from rabbits and laying hens were investigated. In rabbits, the antisera started to be obviously produced after 60 days when the conjugate was firstly injected, reached peaks after 120 days, and began to go down after 135 days; while in laying hens, the antisera started to be largely produced after 90 days of the first injection, arrived to peaks after 135 days, and began to go down after 165 days. The maximum enzyme linked immunosorbent assay(ELISA) titer of rabbits' antisera was up to 1:30,000, whereas the maximum ELISA titer was only 1:8000 in laying hens. There are no apparent difference in the sensitivities and specificities of rabbits' and laying hens' antisera. Both antisera from rabbits and from laying hens can be used as ELISA reagents detecting AFB1 in agricultural commodities.
Chen
F
F
Agro-Microbiology Key Laboratory, Huazhong Agriculture Universty, Wuhan 430070, China.
Zhou
Q
Q
D016604:Aflatoxin B1 / Q000032:analysis* / Q000276:immunology*; D000818:Animals; D000906:Antibodies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D007106:Immune Sera / Q000276:immunology; D008297:Male; D012275:Oryza; D010058:Oviposition; D011817:Rabbits
D000906:Antibodies; D007106:Immune Sera; D016604:Aflatoxin B1