Antioxidant requirements for bovine oocytes varies during in vitro maturation, fertilization and development.
Antioxidants may be beneficial additives to synthetic culture media because these well defined media lack serum or other macromolecules that serve as reactive oxygen species scavengers. In this study, three separate experiments were performed to determine the effects of antioxidants on the development of oocytes to the morula and blastocyst stage when added during in vitro maturation (IVM) of bovine oocytes, during in vitro fertilization (IVF), and during embryo culture for the first 72 h of the development period. Bovine oocytes were matured, fertilized (under 20% O(2)), and embryos were cultured (under 7% O(2)) in defined conditioned medium in vitro with or without supplementation with the antioxidant cysteine, N-acetyl-L-cysteine (NAC), catalase and superoxide dismutase (SOD). Significant improvements in the proportion of oocytes undergoing morula and blastocyst development (33.3% versus 20.3%, P<0.05) were achieved when cysteine (0.6 mM) was added to the maturation medium as compared to control medium without antioxidant supplementation. However, the addition of NAC (0.6mM), catalase (5 or 127 U/ml) or SOD (10 or 1000 U/ml) to the maturation medium did not improve the proportion of oocytes undergoing morula and blastocyst development. During the IVF period, addition of antioxidants (cysteine or NAC 0.6mM, catalase 127U/ml, SOD 100U/ml) significantly reduced the subsequent rate of bovine embryo development to the morula and blastocyst stage (P<0.05). In a defined medium for embryo culture (7% O(2)), the addition of cysteine improved the development of bovine embryos while NAC, catalase and SOD had no positive effect on embryonic development. Our study showed that medium supplementation with cysteine during IVM and in vitro culture (IVC) improved the rate of bovine embryo development, in contrast to extracellular antioxidants like catalase and SOD that caused no improvement.
Ali
A A
AA
Department of Animal Science, Centre de Recherche en Biologie de la Reproduction (CRBR), Laval University, Quebec, Canada G1K 7P4.
Bilodeau
J F
JF
Sirard
M A
MA
D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology; D002374:Catalase / Q000494:pharmacology; D002417:Cattle / Q000196:embryology*; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D003545:Cysteine / Q000494:pharmacology; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005307:Fertilization in Vitro / Q000662:veterinary; D016166:Free Radical Scavengers / Q000378:metabolism; D009028:Morula / Q000187:drug effects / Q000502:physiology; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase / Q000494:pharmacology
D000975:Antioxidants; D003470:Culture Media; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D002374:Catalase; D013482:Superoxide Dismutase; D003545:Cysteine; D000111:Acetylcysteine