Common sites of retroviral integration in mouse hematopoietic tumors identified by high-throughput, single nucleotide polymorphism-based mapping and bacterial artificial chromosome hybridization.
Retroviral insertional mutagenesis in mouse hematopoietic tumors provides a powerful cancer gene discovery tool. Here, we describe a high-throughput, single nucleotide polymorphism (SNP)-based method, for mapping retroviral integration sites cloned from mouse tumors, and a bacterial artificial chromosome (BAC) hybridization method, for localizing these retroviral integration sites to common sites of retroviral integration (CISs). Several new CISs were identified, including one CIS that mapped near Notch1, a gene that has been causally associated with human T-cell tumors. This mapping method is applicable to many different species, including ones where few genetic markers and little genomic sequence information are available. It can also be used to map endogenous proviruses.
Shen
Haifa
H
Mouse Cancer Genetics Program, National Cancer Institute-Frederick, Maryland 21702, USA.
Suzuki
Takeshi
T
Munroe
David J
DJ
Stewart
Claudia
C
Rasmussen
Lynn
L
Gilbert
Debra J
DJ
Jenkins
Nancy A
NA
Copeland
Neal G
NG
D000818:Animals; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D019337:Hematologic Neoplasms / Q000821:virology*; D051379:Mice; D016254:Mutagenesis, Insertional; D020641:Polymorphism, Single Nucleotide; D012190:Retroviridae / Q000235:genetics / Q000502:physiology*; D016662:Virus Integration