title: Regional distribution and relative frequency of gastrointestinal endocrine cells in large intestines of C57BL/6 mice.
abstract: The regional distributions and relative frequencies of some gastrointestinal endocrine cells in the three portions (cecum, colon and rectum) of the large intestinal tract of C57BL/6 mice were examined with immunohistochemical method using 7 types of specific antisera against chromogranin A (CGA), serotonin, somatostatin, human pancreatic polypeptide (HPP), glucagon, gastrin and cholecyctokinin (CCK)-8. In this study, all 3 types of immunoreactive (IR) cells were identified. Most of these IR cells in the large intestinal portion were generally spherical or spindle in shape (open-typed cell) while cells with a round shape (close-typed cell) were found in the intestinal gland. Their relative frequencies varied according to each portion of the large intestinal tract. CGA-IR cells were found throughout the whole large intestinal tract but were most predominant in the colon. Serotonin-IR cells were detected throughout the whole large intestinal tract and showed highest frequency in the colon. Peculiarly, glucagon-IR cells were restricted to the colon with a low frequency. However, no somatostatin-, HPP-, gastrin- and CCK-8-IR cells were found in the large intestinal tract. In conclusion, some peculiar distributional patterns of large intestinal endocrine cells were identified in C57BL/6 mice.
authors.affiliation: College of Natural Resources, Yeungnam University, Kyungsan, Republic of Korea. shtae@yumail.ac.kr
authors.forename: Tae-Su
authors.initials: TS
authors.lastname: Ham
mesh_terms: D000818:Animals; D053379:Chromogranin A; D002864:Chromogranins / Q000378:metabolism; D019858:Enteroendocrine Cells / Q000378:metabolism* / Q000502:physiology; D005260:Female; D005755:Gastrins / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D007150:Immunohistochemistry / Q000662:veterinary; D007420:Intestine, Large / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010191:Pancreatic Polypeptide / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D012844:Sincalide / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism
chemical_list: D053379:Chromogranin A; D002864:Chromogranins; D005755:Gastrins; D012701:Serotonin; D013004:Somatostatin; D010191:Pancreatic Polypeptide; D005934:Glucagon; D012844:Sincalide