Physical methods.
Despite the advent of high-output, recombinant DNA-based screening strategies, many important protein-protein interactions in the plant cell have been and still are revealed using co-sedimentation, affinity chromatography and other affinity techniques, co-immunoprecipitation and cross-linking. The advantages of these techniques, the care that should be taken interpreting the data obtained and the possible ways to overcome pitfalls are illustrated.
Vitale
Alessandro
A
Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milano, Italy. vitale@ibv.mi.cnr.it
D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003432:Cross-Linking Reagents / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding / Q000187:drug effects; D025941:Protein Interaction Mapping / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D003432:Cross-Linking Reagents; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins