[Hairy root induction and in vitro cultivation of Pueraria lobata willa. ohwi].
OBJECTIVE
To study the hairy root induction and hormone-free in vitro liquid cultivation of Pueraria lobata (Willd) Ohwi.


METHODS
Co-cultivation of super-virulent Agrobacterium rhizogenes R1601 with P. lobata leaves in vitro.


RESULTS
Hairy roots of rapid growth, high branches and plagiotropism developed vigorously on the surface of leaves, exhibiting rapid growth and resistance to kanamycin in hormone-free medium in vitro.


CONCLUSIONS
A method of hairy root induction with A. rhizogenes as well as a system hairy root liquid cultivation in vitro for P. lobata have been established.
Liu
C F
CF
Agronomy College of Jiangxi Agricultural University, Nanchang 330045 Jiangxi, China.
Li
L
L
Shi
H P
HP
Pan
R C
RC
Yu
S H
SH
D018920:Coculture Techniques; D003470:Culture Media; D018515:Plant Leaves / Q000254:growth & development; D018517:Plant Roots / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*; D029908:Pueraria / Q000254:growth & development*; D012231:Rhizobium / Q000502:physiology*
D003470:Culture Media