title: Photopolymerized hyaluronic acid-based hydrogels and interpenetrating networks.
abstract: Hyaluronic acid (HA) was derivatized with methacrylic esters used for the preparation of hydrogels via photopolymerization. Poly(ethylene glycol) diacrylate (PEG-DA) with a molecular weight of 570 was also used as a comacromonomer to improve elastic modulus and swelling behavior. The hydrogels were readily degraded by hyaluronidase and their mechanical properties could be modulated by HA molecular weight and concentration of PEG-DA. The incorporation of RGD peptides allowed modulation of the HA properties from cell non-adhesive to adhesive. Human dermal fibroblasts were cultured on the RGD, RDG, and non-functionalized HA hydrogels for up to 7d, showing adhesion and proliferation only with incorporated RGD.
authors.affiliation: Department of Materials, Institute for Biomedical Engineering, Swiss Federal Institute of Technology and University of Zürich, Moussonstrasse 18, CH-8044 Zürich, Switzerland.
authors.forename: Yong Doo
authors.initials: YD
authors.lastname: Park
authors.forename: Nicola
authors.initials: N
authors.lastname: Tirelli
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Hubbell
mesh_terms: D000595:Amino Acid Sequence; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004548:Elasticity; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D006821:Hyaluronoglucosaminidase; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry*; D007202:Indicators and Reagents; D008970:Molecular Weight; D010777:Photochemistry; D011092:Polyethylene Glycols; D012867:Skin / Q000166:cytology
chemical_list: D020100:Hydrogels; D007202:Indicators and Reagents; C437167:poly(ethylene glycol)diacrylate; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase