Differential expression of class I MHC mRNA in the hypothalamus of Lewis and Fischer rats.
The expression of class I major histocompatibility complex (MHC) mRNA was compared in the brains of inflammatory susceptible, hypothalamic-pituitary-adrenal (HPA)-axis blunted Lewis rats and inflammatory resistant, HPA-axis hyperresponsive Fischer rats by quantitative in situ hybridization following lipopolysaccharide (LPS) or vehicle administration. Lewis rats showed lower levels of class I MHC in the hypothalamic paraventricular nucleus (PVN) and retrochiasmatic area (RCA) compared to Fischer rats under both vehicle and LPS conditions. No differences were detected in other brain structures. Differential class I MHC expression in Lewis rats suggests an alteration in mechanisms related to cellular activity of neuroendocrine cells rather than alterations specific to neuroendocrine molecules.
Puchowicz
Michael
M
Section of Neuroendocrine Immunology and Behavior, National Institute of Mental Health, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA.
Tonelli
Leonardo H
LH
Sternberg
Esther M
EM
D000818:Animals; D002831:Choroid Plexus / Q000276:immunology / Q000378:metabolism; D004198:Disease Susceptibility / Q000276:immunology*; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D007030:Hypothalamo-Hypophyseal System / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D007031:Hypothalamus / Q000276:immunology* / Q000378:metabolism; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D015213:Neuroimmunomodulation / Q000235:genetics / Q000276:immunology; D009474:Neurons / Q000276:immunology / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000276:immunology / Q000378:metabolism; D010913:Pituitary-Adrenal System / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D013356:Subfornical Organ / Q000276:immunology / Q000378:metabolism
D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger