title: A natural compound (reuterin) produced by Lactobacillus reuteri for biological-tissue fixation.
abstract: The study was undertaken to examine the degree of tissue fixation by reuterin, a natural compound produced by Lactobacillus reuteri, at distinct fixation conditions (pH, temperature, and fixative concentration). Additionally, the rate of tissue fixation by reuterin was investigated using glutaraldehyde as a control. It was found by the Fourier transformed infrared spectroscopy and nuclear magnetic resonance spectroscopy that both mono- and di-aldehyde reuterin oligomers may be present in the acidic and basic aqueous reuterin solutions. Therefore, reuterin may crosslink biological tissues as glutaraldehyde (a di-aldehyde agent). The degree of tissue fixation by reuterin is significantly affected by its fixation conditions. Generally, with increasing the pH, temperature, or fixative concentration, the reduction in free-amino-group content, denaturation temperature, tensile strength, and resistance against enzymatic degradation of the reuterin-fixed tissue increased significantly. Also, the rate of tissue fixation by reuterin is significantly slower than that by glutaraldehyde. However, after fixation, it was noted that the reuterin-fixed tissue has comparable free-amino-group content, denaturation temperature, tensile strength, and resistance against enzymatic degradation as the glutaraldehyde-fixed tissue.
authors.affiliation: Department of Chemical Engineering, National Tsing Hua University, Hsinchu, 30013, Taiwan. hwsung@che.nthu.edu.tw
authors.forename: Hsing-Wen
authors.initials: HW
authors.lastname: Sung
authors.forename: Chiun-Nan
authors.initials: CN
authors.lastname: Chen
authors.forename: Hsiang-Fa
authors.initials: HF
authors.lastname: Liang
authors.forename: Min-Hao
authors.initials: MH
authors.lastname: Hong
mesh_terms: D000447:Aldehydes; D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D001688:Biological Products / Q000737:chemistry; D001705:Bioprosthesis; D003432:Cross-Linking Reagents / Q000737:chemistry; D005404:Fixatives / Q000737:chemistry; D005976:Glutaral; D005985:Glyceraldehyde / Q000031:analogs & derivatives; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000378:metabolism; D008422:Materials Testing; D019906:Nuclear Magnetic Resonance, Biomolecular; D010496:Pericardium; D011407:Propane; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D034421:Sus scrofa; D013696:Temperature; D013718:Tensile Strength; D016707:Tissue Fixation / Q000379:methods*; D014867:Water
chemical_list: D000447:Aldehydes; D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D001688:Biological Products; D003432:Cross-Linking Reagents; D005404:Fixatives; D012996:Solutions; D014867:Water; C047158:3-hydroxypropionaldehyde; D005985:Glyceraldehyde; D005976:Glutaral; D011407:Propane