Technique for producing platelet-rich plasma and platelet concentrate: background and process.
Autologous platelet-rich plasma is a source of platelet-derived growth factor (PDGF) and transforming growth factor beta (TGF-beta), both important in accelerating hard and soft tissue maturation. This article describes a two-step centrifugation method to sequester and concentrate platelets to a level four to eight times baseline whole blood values. The technique produces concentrations of PDGF-AB above 500% and TGF-beta1 greater than 800%. Flow cytometry measuring p-selectin expression shows that the platelets remain quiescent throughout the procedure, maintaining their integrity and viability, with no inadvertent activation.
Gonshor
Aron
A
arongonshor@hotmail.com
D002498:Centrifugation; D005782:Gels / Q000138:chemical synthesis; D006801:Humans; D019007:P-Selectin / Q000032:analysis; D010949:Plasma; D015539:Platelet Activation; D010982:Platelet-Derived Growth Factor / Q000032:analysis; D010983:Plateletpheresis / Q000379:methods*; D016212:Transforming Growth Factor beta / Q000032:analysis
D005782:Gels; D019007:P-Selectin; D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta