[Sequence analysis of RNA3 of Rice stripe virus isolates found in China: evidence for reassortment in Tenuivirus].
The RNA3 segments of four isolates of Rice stripe virus (RSV), isolated from endemic sites at Panjin (PJ), Liaoning Province, Kunming (KM) and Yiliang (YL), Yunnan Province, as well as from outbreak sites at Hongze (HZ), Jiangsu Province, were determined. RNA3 of these four isolates were 2480 bp, 2509 bp, 2489 bp and 2497 bp in length, respectively. Compared with RNA3 of T and M isolates from Japan and Y isolate from Yunnan Province of China, that had been previously reported, these seven isolates could be divided into two groups. KM and YL isolates formed group one, and PJ, HZ, Y, T and M isolates belonged to another group. The two groups shared 97%-98% and 93%-94% sequence homology in viral RNA3 (vRNA3) and viral complementary RNA3 (vcRNA3) at the nucleotide levels, respectively, and there was no significant difference between the two groups at the amino acid levels. In the first group, Y isolate was significantly different from HZ,PJ and two Japan isolates in their RNA4 segment. These results show that there were two subgroups in RSV natural population related with geographical location, and reassorment may be the main factor leading to different segments of Y isolate belonging to different subgroups. The results may provide another evidence for reassortment variation in Tenuivirus.
Wei
Tai-Yun
TY
Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Wang
Hui
H
Lin
Han-Xin
HX
Wu
Zu-Jian
ZJ
Lin
Qi-Ying
QY
Xie
Lian-Hui
LH
D001483:Base Sequence; D002681:China; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D029364:Tenuivirus / Q000235:genetics* / Q000302:isolation & purification
D018076:DNA, Complementary; D012367:RNA, Viral