[Effect of expression of gut-enriched Kruppel-like factor on esophageal squamous cancer cells].
Gut-enriched Kruppel-like factor (GKLF) is a newly identified eukaryotic zinc finger protein expressed extensively in the gastrointestinal tract, and its expression is associated with growth arrest. Previous work showed that GKLF expression was down-regulated in esophageal squamous cancer. In the present work, the effects of sense and antisense expression of GKLF on the esophageal squamous cancer cell line EC9706 were observed to get more knowledge of the role of down-regulation of GKLF in the initiation and/or progression of esophageal cancer. Antisense transfection of GKLF gene in EC9706 led to decreased expression of GKLF in the cells, and these cells showed increased cell growth rate, indicating that down-regulation of the GKLF gene might contribute to cellular hyperproliferation. Both increased and decreased expression of GKLF in EC9706 by sense and antisense GKLF expression also led to the decreased adhesion of cells, suggesting a role of GKLF in the metastasis of esophageal squamous cancer. The results demonstrate that down-regulation of GKLF expression in esophageal squamous cancer causes the cancer cells to become hyperproliferative and less adhesive, and suggest that decreased expression of GKLF may contribute to the malignant phenotype of esophageal squamous cancer.
Wang
Nan
N
National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, Beijing 100021, China.
Liu
Zhi-Hua
ZH
Ding
Fang
F
Wang
Xiu-Qin
XQ
Wu
Min
M
D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D016373:DNA, Antisense / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D004938:Esophageal Neoplasms / Q000235:genetics / Q000473:pathology*; D006801:Humans; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D016373:DNA, Antisense; D004268:DNA-Binding Proteins; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors