Conditional replication of duck hepatitis B virus in hepatoma cells.
To facilitate investigations of replication and host cell interactions in the hepadnavirus system, we have developed cell lines permitting the conditional replication of duck hepatitis B virus (DHBV). With the help of this system, we devised conditions for core particle isolation that preserve replicase activity, which was not found in previous preparations. Investigations of the stability of viral DNA intermediates indicated that both encapsidated DNA and covalently closed circular DNA (cccDNA) were turned over independently of cell division. Moreover, we showed that alpha interferon reduced the accumulation of RNA-containing viral particles. The availability of a synchronized replication system will permit the biochemical analysis of individual steps of the viral replication cycle, including the mechanism and regulation of cccDNA formation.
Guo
Ju-Tao
JT
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.
Pryce
Melissa
M
Wang
Xingtai
X
Barrasa
M Inmaculada
MI
Hu
Jianming
J
Seeger
Christoph
C
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000821:virology*; D002645:Chickens; D004270:DNA, Circular / Q000737:chemistry; D004279:DNA, Viral / Q000096:biosynthesis; D015611:Hepatitis B Virus, Duck / Q000502:physiology*; D008113:Liver Neoplasms / Q000821:virology*; D012367:RNA, Viral / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D004270:DNA, Circular; D004279:DNA, Viral; D012367:RNA, Viral