Drug targeting Mycobacterium tuberculosis cell wall synthesis: development of a microtiter plate-based screen for UDP-galactopyranose mutase and identification of an inhibitor from a uridine-based library.
A microtiter plate assay for UDP-galactopyranose mutase, an essential cell wall biosynthetic enzyme of Mycobacterium tuberculosis, was developed. The assay is based on the release of tritiated formaldehyde from UDP-galactofuranose but not UDP-galactopyranose by periodate and was used to identify a uridine-based enzyme inhibitor from a chemical library.
Scherman
Michael S
MS
Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA.
Winans
Katharine A
KA
Stern
Richard J
RJ
Jones
Victoria
V
Bertozzi
Carolyn R
CR
McNeil
Michael R
MR
D002473:Cell Wall / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000494:pharmacology; D019751:Intramolecular Transferases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000201:enzymology*; D013329:Structure-Activity Relationship
D004791:Enzyme Inhibitors; D019751:Intramolecular Transferases; C098051:UDP-galactopyranose mutase