Contribution of ferrous iron to maintenance of the gastric colonization of Helicobacter pylori in miniature pigs.
Our previous study showed that the colonization levels of Helicobacter pylori were higher in the stomachs of 5-day-old miniature pigs than in 2-week-old ones. As dietary factors can cause these differences, we compared two diets, i.e., Weanymilk and a similar formula with a higher concentration of Fe(II), Weanylobulin. The colonization levels in the fundic mucosa were significantly higher in 2-week-old pigs fed Weanylobulin than in those fed Weanymilk. Supplementing Weanylobulin with an iron chelator, deferoxamine mesylate, significantly lowered the bacteria counts in the gastric mucosa. Normal diets supplemented with Fe(II) in 2-month-old pigs caused significantly more sites of bacteria in the antrum compared with normal diets alone. In addition, ranitidine, an inhibitor of gastric acid secretion that reduces Fe(III) to Fe(II) in the stomach, decreased the bacteria counts in 10-month-old pigs. These results suggested that Fe(II) maintained the colonization levels of H. pylori in the stomach of the miniature pigs.
Koga
Tetsufumi
T
Biological Research Laboratories, Sankyo Co., Ltd., Shinagawa-ku, Tokyo, Japan. tekoga@shina.sankyo.co.jp
Shimada
Yukio
Y
Sato
Kiyoshi
K
Takahashi
Keigo
K
Kikuchi
Isamu
I
Okazaki
Yoko
Y
Miura
Tomoko
T
Katsuta
Mitsuo
M
Iwata
Masayuki
M
D000818:Animals; D015169:Colony Count, Microbial; D003676:Deferoxamine / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000382:microbiology*; D016480:Helicobacter pylori / Q000254:growth & development*; D007505:Iron-Dextran Complex / Q000494:pharmacology; D008297:Male; D011899:Ranitidine / Q000494:pharmacology; D013552:Swine; D013556:Swine, Miniature
D005296:Ferrous Compounds; D011899:Ranitidine; D007505:Iron-Dextran Complex; D003676:Deferoxamine