A new method to enhance contrast of ultrathin cryosections for immunoelectron microscopy.
Adequate contrast of ultrathin cryosections is crucial for evaluating morphological detail to assess immunocytochemical localization at the electron microscopic level. We have developed a positive staining method for achieving contrast in ultrathin cryosections, from tissue fixed only in paraformaldehyde, that provides excellent contrast at the electron microscopic level.
Takizawa
Toshihiro
T
Department of Physiology, Ohio State University, Columbus, Ohio 43210, USA. takizawa.2@osu.edu
Anderson
Clark L
CL
Robinson
John M
JM
D051242:Caveolin 1; D022461:Caveolins / Q000032:analysis; D002478:Cells, Cultured; D005404:Fixatives; D005557:Formaldehyde; D005629:Frozen Sections; D006801:Humans; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron / Q000379:methods*; D010920:Placenta / Q000737:chemistry / Q000648:ultrastructure; D011108:Polymers; D013194:Staining and Labeling / Q000379:methods*; D014471:Umbilical Veins / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure
D051242:Caveolin 1; D022461:Caveolins; D005404:Fixatives; D011108:Polymers; D005557:Formaldehyde; C003043:paraform