title: Isolation and characterization of Shiga toxin-producing Escherichia coli O157:H7 from beef, pork and cattle fecal samples in Changchun, China.
abstract: Meat samples and fecal specimens from adult cattle were collected in Changchun, China and were examined for presence of Shiga toxin-producing Escherichia coli (STEC) serogroup O157. STEC O157 strains were isolated from 2 (5%) of 40 beef, 1 (3.3%) of 30 pork, and 3 (1.7%) of 176 adult cattle fecal samples. The strains belonged to phage types (PT) 4, 8, or 47. Two beef strains and a strain previously isolated from a patient in Shandong, China, were PT-4 and showed a similar PFGE pattern, suggesting the possibility of food-borne transmission. It is suggested that cattle are a reservoir of STEC O157:H7 and meat products are contaminated by this pathogen in Changchun, China as well as in other countries.
authors.affiliation: Faculty of Animal Medicine, Changchun University of Agriculture and Animal Sciences, Changchun, China.
authors.forename: Zhijiang
authors.initials: Z
authors.lastname: Zhou
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.forename: Shangyuan
authors.initials: S
authors.lastname: Hong
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hase
authors.forename: Tom
authors.initials: T
authors.lastname: Cheasty
authors.forename: Henry R
authors.initials: HR
authors.lastname: Smith
authors.forename: Minggong
authors.initials: M
authors.lastname: Zheng
authors.forename: Kosuke
authors.initials: K
authors.lastname: Haruki
mesh_terms: D000818:Animals; D002417:Cattle / Q000382:microbiology*; D002681:China; D004197:Disease Reservoirs / Q000662:veterinary; D019453:Escherichia coli O157 / Q000302:isolation & purification* / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D022621:Shiga Toxin / Q000096:biosynthesis*; D013552:Swine / Q000382:microbiology*
chemical_list: D022621:Shiga Toxin