title: Drug targeting Mycobacterium tuberculosis cell wall synthesis: development of a microtiter plate-based screen for UDP-galactopyranose mutase and identification of an inhibitor from a uridine-based library.
abstract: A microtiter plate assay for UDP-galactopyranose mutase, an essential cell wall biosynthetic enzyme of Mycobacterium tuberculosis, was developed. The assay is based on the release of tritiated formaldehyde from UDP-galactofuranose but not UDP-galactopyranose by periodate and was used to identify a uridine-based enzyme inhibitor from a chemical library.
authors.affiliation: Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Scherman
authors.forename: Katharine A
authors.initials: KA
authors.lastname: Winans
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Stern
authors.forename: Victoria
authors.initials: V
authors.lastname: Jones
authors.forename: Carolyn R
authors.initials: CR
authors.lastname: Bertozzi
authors.forename: Michael R
authors.initials: MR
authors.lastname: McNeil
mesh_terms: D002473:Cell Wall / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000494:pharmacology; D019751:Intramolecular Transferases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000201:enzymology*; D013329:Structure-Activity Relationship
chemical_list: D004791:Enzyme Inhibitors; D019751:Intramolecular Transferases; C098051:UDP-galactopyranose mutase