[Effects of beta-carotene and vitamin C on the expression of c-myc in human leukemic cell].
In order to study the effects of beta-carotene and vitamin C(VC) on the expression of c-myc oncogene in human leukemic cell, myelogenous leukemic cell line HL-60 was cultured in vitro with standard methods. Trypan blue exclusion test and Northern blotting hybridization were used to detect the cell proliferation and the expression of c-myc gene. The results showed that the proliferation of HL-60 cell was inhibited after exposed to VC(5, 10, 100 mumol/L) and the occurrence of proliferation was earlier at lower concentration of VC(5 mumol/L). beta-carotene at 10-100 mumol/L could inhibit the proliferation of HL-60 cell in a dose dependent manner. VC(5 mumol/L) had no influence on the expression of c-myc gene(P > 0.05), but beta-carotene could up-regulate the expression of c-myc(P < 0.05). Our observations suggested that the inhibitory effect of beta-carotene on the proliferation of leukemic cells was caused by inducing apoptosis of HL-60 through up-regulating the expression of c-myc gene; however, the inhibitory effect of vitamin C on cell proliferation was not related to the expression of c-myc gene.
Zhang
J
J
Department of Biology, Medical College, Tongji University, Shanghai 200331, China.
Zhang
J
J
Zhao
Y
Y
Shi
H
H
D001205:Ascorbic Acid / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D018922:HL-60 Cells / Q000378:metabolism*; D006801:Humans; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D019207:beta Carotene / Q000494:pharmacology*
D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D019207:beta Carotene; D001205:Ascorbic Acid