The leukocyte antigen CD43 is expressed in different cell lines of nonhematopoietic origin.
CD43 is an abundant transmembrane sialoglycoprotein in leukocyte-type cell lines, but it has also been suggested to be present in colon adenomas and colon carcinomas. We have now shown that CD43 is expressed in a variety of cell lines of different origins (CaSKI, A549, 293, MTSV1-7, MCF7, HT-1080, Jurkat, K562, COLO 205, HT-29, Caco-2, DLD-1 and SW480). The level of expression of CD43 mRNA was analyzed by reverse transcriptase-polymerase chain reaction and that of the protein by immunoprecipitation and Western blot, flow cytometry and confocal microscopy using two monoclonal anti-CD43 antibodies (L10 and 4D2). As all cell lines expressed CD43, it is suggested that CD43 has a more fundamental function than previously believed and thus cannot be considered only as a specific leukocyte marker.
Fernandez-Rodriguez
J
J
Department of Medical Biochemistry, Göteborg University, Gothenburg, Sweden.
Andersson
C X
CX
Laos
S
S
Baeckström
D
D
Sikut
A
A
Sikut
R
R
Hansson
G C
GC
D000911:Antibodies, Monoclonal / Q000378:metabolism; D015703:Antigens, CD; D015153:Blotting, Western; D002460:Cell Line; D002469:Cell Separation; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D005434:Flow Cytometry; D006801:Humans; D007438:Introns; D019169:Jurkat Cells; D051917:Leukosialin; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000378:metabolism; D011233:Precipitin Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000096:biosynthesis* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D051917:Leukosialin; C465014:SPN protein, human; D012795:Sialoglycoproteins