title: [Morphological observation of mesenchymal stem cells cultured with allogenic decalcified bone matrix].
abstract: OBJECTIVE
  To investigate the feasibility of using decalcified bone matrix loaded with mesenchymal stem cells (MSCs) derived from adult human bone marrow as scaffolds for bone tissue engineering.
  
  
  METHODS
  Allogenic decalcified bone matrix was prepared. MSCs were isolated from adult human bone marrow with monocyte-separating medium and then cultured in basic medium of mesenchymal stem cells (MSC). The third generation of the cultured cells were identified by FITC-antiCD105, seeded onto allogenic decalcified bone matrix followed by observation under scanning electron microscope and invert microscope a week later.
  
  
  RESULTS
  Rapid proliferation of both primary and subcultured MSCs was observed, and CD105-positive cells reached 64.1% among the third generation of the cells. MSCs also grew well and proliferated rapidly after being cultured with decalcified bone matrix.
  
  
  CONCLUSIONS
  Naive MSCs are ideal seeding cells for bone tissue engineering, and the allogenic decalcified bone matrix loaded with MSCs may be used as a good scaffold.
authors.affiliation: Department of Anatomy First Military Medical University, Guangzhou 510515, China.
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Liao
mesh_terms: D001857:Bone Matrix / Q000502:physiology*; D002455:Cell Division; D002478:Cells, Cultured; D006801:Humans; D008648:Mesoderm / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D013234:Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D023822:Tissue Engineering / Q000379:methods*