[The effect of heparin on mesangial cells proliferation and cytokines production].
The human glomerular mesangial cells(MC) proliferation and cytokines secretion were detected by using MTT method and biological active method so as to assess the effects of heparin on the cultured human glomerular MC induced by lipopolysaccharides (LPS). The results showed that there were basic production of interleukin-6(IL-6) and tumor necrosis factor-alpha (TNF-alpha) on MC in the control group without LPS, that high-concentration heparin(500 U/ml) increased the effects of LPS on MC, and that low-concentration heparin (5 U/ml) conversely inhibited the effects of LPS on MC. These data indicate that the low-concentration heparin could inhibit the proliferation of MC and the secrection of IL-6 and TNF-alpha induced by LPS, and hence provide an experimental evidence for administration of heparin in the treatment glomerular disease.
Liu
X
X
Department of Nophrology, First Affiliated Hospital, WCUMS, Chengdu 610041.
Qu
S
S
Qiu
H
H
Yang
L
L
D002478:Cells, Cultured; D005920:Glomerular Mesangium / Q000166:cytology* / Q000378:metabolism; D006493:Heparin / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D006493:Heparin