[Effects of components isolated from Astragalus membranaceus Bunge on cardiac function injured by myocardial ischemia reperfusion in rats].
OBJECTIVE
To investigate the effects of components isolated from Astragalus membranaceus on myocardial ischemia reperfusion injury.


METHODS
The myocardial ischemia reperfusion injury model was created by the left anterior descending coronary artery occlusion from the oracotouated rats, and the total saponins, total flavonids and astragaloside i.v. isolated from A. membranaceus on hemodynamics during acute myocardial ischemia, Na(+)-K(+)-ATPase activity, cAMP and malondialdehyede (MDA) contents in the ischemic myocardium were observed.


RESULTS
The total saponins, total flavonids and astragaloside i.v. attenuated the declines of the amplitudes of LVSP and +/- LVdp/dtmax in rat heart injured by ischemia reperfusion in vivo, and decreased Na(+)-K(+)-ATPase activity in the ischemic myocardium. Otherwise, the total saponins increased the cAMP content and the total flavonids decreased the level of MDA production in the ischemic myocardium.


CONCLUSIONS
The effects of different components isolated from A. membranaceus on protecting the cardiac function in the process of ischemia reperfusion may be related to the mechanism of improving energy metabolism, scavenging the oxygen free radicals and inhibiting the production of free radicals in the ischemic myocardium.
Zhou
J Y
JY
Scientific Research Office, Shanghai University of TCM, Shanghai 200032, China.
Fan
Y
Y
Kong
J L
JL
Wu
D Z
DZ
Hu
Z B
ZB
D000818:Animals; D027885:Astragalus propinquus / Q000737:chemistry*; D002316:Cardiotonic Agents / Q000302:isolation & purification / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D006334:Heart Function Tests / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A; D008315:Malondialdehyde; D000254:Sodium-Potassium-Exchanging ATPase