Antioxidant capability and efficacy of Mega-H silica hydride, an antioxidant dietary supplement, by in vitro cellular analysis using photosensitization and fluorescence detection.
Treatment of Chinese hamster ovary and mouse hybridoma cells with Mega-H brand silica hydride, a marketed antioxidant, after photosensitization with singlet oxygen and hydroxyl/superoxide reactive oxygen species through the use of rose bengal diacetate and malachite green resulted in an effective method of reducing free radical activity by more than 96% against singlet oxygen species and more than 86% for hydroxyl and superoxide free radicals with the dosage recommended by the manufacturer. The analysis used a combinational spectrafluorometric technique to determine cell viability and cytotoxicity through the mechanism of intracellular esterase activity and plasma membrane integrity. Photosensitized controls not treated with silica hydride showed less than 1% viability under the same conditions. The reduction of the introduced free radicals and singlet oxygen species and the consequent high levels of cell viability may be the result of effective and efficient antioxidant and radical scavenging properties of silica hydride.
Stephanson
Cory J
CJ
Department of Chemistry, University of Minnesota, Minneapolis, MN, USA.
Stephanson
Anne M
AM
Flanagan
G Patrick
GP
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016466:CHO Cells / Q000166:cytology; D002470:Cell Survival; D004396:Coloring Agents; D006224:Cricetinae; D019587:Dietary Supplements; D005456:Fluorescent Dyes; D006825:Hybridomas / Q000166:cytology; D017665:Hydroxyl Radical; D051379:Mice; D008856:Microscopy, Fluorescence; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010777:Photochemistry; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012394:Rosaniline Dyes; D012395:Rose Bengal; D012822:Silicon Dioxide / Q000494:pharmacology*; D026082:Singlet Oxygen; D013050:Spectrometry, Fluorescence
D000975:Antioxidants; D004396:Coloring Agents; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D012394:Rosaniline Dyes; C005095:malachite green; D026082:Singlet Oxygen; D012395:Rose Bengal; D017665:Hydroxyl Radical; D012822:Silicon Dioxide; D010100:Oxygen