Synthesis and screening of a molecularly imprinted polymer library targeted for penicillin G.
A library of molecularly imprinted polymers (MIPs) was synthesized by radical bulk polymerization using the beta-lactam antibiotic penicillin G as the template. Diversity of the library was obtained by combining various functionalized monomers and cross-linkers and by varying the stoichiometry and the concentration of the components in the prepolymerization mixtures. The library was screened for selectivity to penicillin G by a radioligand binding assay and was compared to a corresponding control library. The best MIP candidate, showing the highest selectivity for penicillin G, was prepared from methacrylic acid and trimethylolpropane trimethacrylate as the functionalized monomer and cross-linker, respectively. Cross-reactivity studies with other beta-lactam antibiotics showed a low cross-reactivity of penicillin V (15%), ampicillin (16%), and amoxicillin (19%). Nafcillin and oxacillin showed less cross-reactivity (<1%). Cross-reaction with a cephalosporin antibiotic (cephapirin) and structurally nonrelated antibiotics (chloramphenicol, tetracycline, dapsone, and erythromycin) was less than 0.01%.
Cederfur
Josefine
J
Biomedical Polymer Technology, Section for Cell and Matrix Biology, Department of Cell and Molecular Biology, Biomedical Center, Lund University, SE-221 84 Lund, Sweden.
Pei
Yuxin
Y
Zihui
Meng
M
Kempe
Maria
M
D000900:Anti-Bacterial Agents / Q000032:analysis; D020650:Combinatorial Chemistry Techniques; D003429:Cross Reactions; D005506:Food Contamination / Q000032:analysis; D010400:Penicillin G / Q000032:analysis*; D011108:Polymers / Q000737:chemistry*; D011869:Radioligand Assay; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D011108:Polymers; D010400:Penicillin G