Correlation between retention and 1-octanol-water partition coefficients of some estrane derivatives in reversed-phase thin-layer chromatography.
The retention constants (RM) of a series of estrane and secoestrane derivatives are experimentally determined on C18-modified silica gel layers with methanol-water mobile phases of various concentrations. The slopes (m) and intercepts (RMW) of the linear relationships between RM and the volume fraction of methanol are calculated. Both constants increase when the retention of compounds increases, and there is a linear dependence between them indicating their additivity; they represent the sums of particular retention contributions of skeleton and substituents. The contributions, particularly the retention fragmental constants, are calculated by combining the linear relationships RM/log P, RM/m, and RMW/log P. The log P values of the compounds and skeleton were calculated using Rekker's fragmental constants.
Petrović
S M
SM
Department of Analytical Chemistry, Faculty of Technology, University of Novi Sad, Bul. Cara Lazara, PO Box 340, 21000 Novi Sad, Yugoslavia.
Loncar
E
E
Kolarov
Lj
Lj
Pejanović
V
V
D020003:1-Octanol / Q000737:chemistry*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004962:Estranes / Q000737:chemistry / Q000302:isolation & purification*; D014867:Water / Q000737:chemistry*
D004962:Estranes; D014867:Water; D020003:1-Octanol