title: Identification of Candida spp. by randomly amplified polymorphic DNA analysis and differentiation between Candida albicans and Candida dubliniensis by direct PCR methods.
abstract: Because Candida species have innately highly variable antifungal susceptibilities, the availability of a fast and reliable species identification test is very important so that suitable and effective therapeutic measures may be taken. Using three oligonucleotide primers, we established a randomly amplified polymorphic DNA (RAPD) analysis method that enabled direct identification of the most common opportunistic pathogenic Candida species. RAPD analysis revealed a characteristic molecular fingerprint for each Candida species. Differences between the profiles for Candida albicans and C. dubliniensis were evident. RAPD analysis is a relatively easy, reproducible, and reliable technique that can be useful in providing genetic fingerprints for the identification of strains. In addition, a collection of different C. albicans strains was identified by a specific PCR based on multiple secreted aspartic proteinase (SAP) genes and the dipeptidyl aminopeptidase (DAP2) gene. Our findings demonstrate that PCR based upon the SAP and DAP2 sequences is a simple, rapid, clear, and direct technique for the identification and differentiation of C. albicans and C. dubliniensis.
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, I.P.N., Mexico D.F., Mexico.
authors.forename: Consuelo
authors.initials: C
authors.lastname: Bautista-Muñoz
authors.forename: Xavier M
authors.initials: XM
authors.lastname: Boldo
authors.forename: Lourdes
authors.initials: L
authors.lastname: Villa-Tanaca
authors.forename: César
authors.initials: C
authors.lastname: Hernández-Rodríguez
mesh_terms: D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002176:Candida albicans / Q000235:genetics / Q000302:isolation & purification; D004271:DNA, Fungal / Q000032:analysis*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
chemical_list: D004271:DNA, Fungal