title: Novel reversible indole-3-carboxylate decarboxylase catalyzing nonoxidative decarboxylation.
abstract: After enrichment culture with indole-3-carboxylate in static culture, a novel reversible decarboxylase, indole-3-carboxylate decarboxylase, was found in Arthrobacter nicotianae FI1612 and several molds. The enzyme reaction was examined in resting-cell reactions with A. nicotianae FI1612. The enzyme activity was induced specifically by indole-3-carboxylate, but not by indole. The indole-3-carboxylate decarboxylase of A. nicotianae FI1612 catalyzed the nonoxidative decarboxylation of indole-3-carboxylate into indole, and efficiently carboxylated indole and 2-methylindole by the reverse reaction. In the presence of 1 mM dithiothreitol, 50 mM Na2 S2O3, and 20% (v/v) glycerol, indole-3-carboxylate decarboxylase was partially purified from A. nicotianae FI1612. The purified enzyme had a molecular mass of approximately 258 kDa. The enzyme did not need any cofactor for the decarboxylating and carboxylating reactions.
authors.affiliation: Department of Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan.
authors.forename: Toyokazu
authors.initials: T
authors.lastname: Yoshida
authors.forename: Kohei
authors.initials: K
authors.lastname: Fujita
authors.forename: Toru
authors.initials: T
authors.lastname: Nagasawa
mesh_terms: D001173:Arthrobacter / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D002262:Carboxy-Lyases / Q000302:isolation & purification / Q000378:metabolism*; D002384:Catalysis; D003653:Decarboxylation; D004229:Dithiothreitol / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005658:Fungi / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D007211:Indoles / Q000378:metabolism* / Q000494:pharmacology; D013379:Substrate Specificity; D015003:Yeasts / Q000166:cytology / Q000254:growth & development / Q000378:metabolism
chemical_list: D007211:Indoles; C012382:indole-3-carboxylic acid; D002262:Carboxy-Lyases; D004229:Dithiothreitol