title: Use of polymerase chain reaction for diagnosis of disseminated adenovirus infection.
abstract: We report a fatal case of disseminated adenovirus infection with fulminant hepatic failure in an 8-month-old child after peripheral blood stem cell transplantation. The virus was identified in blood, urine, respiratory aspirate and stool samples and was typed as adenovirus type 2 through PCR and sequencing of part of the hexon gene, with the use of degenerated consensus primers.
authors.affiliation: Laboratory of Clinical Virology, Rega Institute for Medical Research, University of Leuven, Belgium.
authors.forename: Annabel
authors.initials: A
authors.lastname: Rector
authors.forename: Nadira
authors.initials: N
authors.lastname: Azzi
authors.forename: Corinne
authors.initials: C
authors.lastname: Liesnard
authors.forename: Kalina
authors.initials: K
authors.lastname: Zlateva
authors.forename: Daniele
authors.initials: D
authors.lastname: Van Beers
authors.forename: Robert
authors.initials: R
authors.lastname: Snoeck
authors.forename: Marc
authors.initials: M
authors.lastname: Van Ranst
mesh_terms: D000257:Adenoviridae Infections / Q000175:diagnosis* / Q000628:therapy; D000260:Adenoviruses, Human / Q000302:isolation & purification*; D004279:DNA, Viral / Q000097:blood*; D018450:Disease Progression; D017809:Fatal Outcome; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D006801:Humans; D007223:Infant; D015479:Leukemia, Myelomonocytic, Acute / Q000175:diagnosis / Q000276:immunology / Q000628:therapy; D008297:Male; D009102:Multiple Organ Failure; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D014766:Viremia / Q000175:diagnosis* / Q000628:therapy
chemical_list: D004279:DNA, Viral