Molecular typing of Aeromonas isolates in natural mineral waters.
A total of 103 isolates of Aeromonas spp. were obtained over a 3-year period from a natural mineral water and from surface streams located within the boundaries of the watershed of the natural mineral water wells and were typed by macrorestriction analysis of genomic DNA with XbaI and by pulsed-field gel electrophoresis. All Aeromonas caviae isolates from the natural mineral water belonged to the same clone, and an analogous clonal identity was found among Aeromonas hydrophila isolates. These two clones expressed no hemolytic or cytotoxic activities. Aeromonas isolates from surface waters showed high molecular heterogeneity and were not related to the clones found in the natural mineral water. The presence of aeromonads chronically found in the natural mineral water was a likely consequence of a localized development of a biofilm, with no exogenous contamination of the aquifer. Molecular fingerprinting of drinking water isolates is a useful tool in explaining possible reasons for bacterial occurrences.
Villari
P
P
Department of Experimental Medicine and Pathology, University La Sapienza, 00161 Rome, Italy. paolo.villari@uniroma1.it
Crispino
M
M
Montuori
P
P
Boccia
S
S
D000333:Aeromonas / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D016980:Aeromonas hydrophila / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D000818:Animals; D015373:Bacterial Typing Techniques; D002522:Chlorocebus aethiops; D003603:Cytotoxins / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D005618:Fresh Water / Q000382:microbiology*; D006461:Hemolysis; D008900:Mineral Waters / Q000382:microbiology*; D014709:Vero Cells; D014774:Virulence; D014881:Water Supply
D003603:Cytotoxins; D004269:DNA, Bacterial; D008900:Mineral Waters; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific