Molecular characterization of natural Erwinia pyrifoliae strains deficient in hypersensitive response.
From necrotic tissue of a Nashi pear tree, 24 Erwinia pyrifoliae strains, found to be identical by pulsed-field gel electrophoresis analysis, were isolated. Thirteen strains were not virulent on immature pears and did not induce a hypersensitive response in tobacco leaves. The defective gene hrpL was complemented with intact genes from E. pyrifoliae and Erwinia amylovora.
Jock
Susanne
S
Max-Planck-Institut für Zellbiologie, Rosenhof, 68526 Ladenburg, Germany.
Kim
Won-Sik
WS
Barny
Marie-Anne
MA
Geider
Klaus
K
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D004268:DNA-Binding Proteins; D016521:Electrophoresis, Gel, Pulsed-Field; D004885:Erwinia / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D031989:Pyrus / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012808:Sigma Factor; D014026:Nicotiana / Q000382:microbiology; D014774:Virulence
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C085855:HrpL protein, Pseudomonas syringae; D012808:Sigma Factor