title: Expression of aquaporin 1 and aquaporin 4 water channels in rat choroid plexus.
abstract: The role of aquaporins in cerebrospinal fluid (CSF) secretion was investigated in this study. Western analysis and immunocytochemistry were used to examine the expression of aquaporin 1 (AQP1) and aquaporin 4 (AQP4) in the rat choroid plexus epithelium. Western analyses were performed on a membrane fraction that was enriched in Na(+)/K(+)-ATPase and AE2, marker proteins for the apical and basolateral membranes of the choroid plexus epithelium, respectively. The AQP1 antibody detected peptides with molecular masses of 27 and 32 kDa in fourth and lateral ventricle choroid plexus. A single peptide of 29 kDa was identified by the AQP4 antibody in fourth and lateral ventricle choroid plexus. Immunocytochemistry demonstrated that AQP1 is expressed in the apical membrane of both lateral and fourth ventricle choroid plexus epithelial cells. The immunofluorescence signal with the AQP4 antibody was diffusely distributed throughout the cytoplasm, and there was no evidence for AQP4 expression in either the apical or basolateral membrane of the epithelial cells. The data suggest that AQP1 contributes to water transport across the apical membrane of the choroid plexus epithelium during CSF secretion. The route by which water crosses the basolateral membrane, however, remains to be determined.
authors.affiliation: School of Biological Sciences, University of Manchester, UK.
authors.forename: Tracey
authors.initials: T
authors.lastname: Speake
authors.forename: Lyle J
authors.initials: LJ
authors.lastname: Freeman
authors.forename: Peter D
authors.initials: PD
authors.lastname: Brown
mesh_terms: D000818:Animals; D051398:Aquaporin 1; D051401:Aquaporin 4; D020346:Aquaporins / Q000378:metabolism*; D015153:Blotting, Western; D002831:Choroid Plexus / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C496022:Aqp1 protein, rat; C496032:Aqp4 protein, rat; D051401:Aquaporin 4; D020346:Aquaporins; D051398:Aquaporin 1