title: Simple and sensitive determination of the new antitumor drug CKD-602 in human plasma by liquid chromatography.
abstract: A simple and sensitive high-performance liquid chromatographic with fluorescence detection method has been developed and validated for the determination of the new antitumor agent CKD-602 in human plasma. Plasma proteins were precipitated with methanol and the samples were acidified with 7% (v/v) perchloric acid. The supernatants were analyzed by HPLC using a Capcell Pak C(18) UG120 column and a mixture of methanol-0.1 M hexane-1-sulfonic acid in methanol-0.01 M TEMED in water at pH 6.0 (40:1:59, v/v) as the mobile phase. The lower limit of quantification was 0.2 ng/ml using 200 microliter plasma samples. Mean within-run precision and between-run precision at six tested concentrations (0.2-400 ng/ml) were </=10% and mean accuracy was </=15%. Stability studies showed that CKD-602 is stable in both plasma and methanol extracts for at least 3 months at -30 degrees C. The described method was used for the pharmacokinetic analysis of CKD-602 during clinical phase I studies, in patients with solid tumors.
authors.affiliation: Department of Pharmacology, Seoul National University College of Medicine and Hospital, 28 Yongun-dong, Chongro-gu, 110-799, Seoul, South Korea. jycho@cpt.snu.ac.kr
authors.forename: Joo Youn
authors.initials: JY
authors.lastname: Cho
authors.forename: Hyo Bum
authors.initials: HB
authors.lastname: Seo
authors.forename: Kyung Sang
authors.initials: KS
authors.lastname: Yu
authors.forename: Kyun Seop
authors.initials: KS
authors.lastname: Bae
authors.forename: So Young
authors.initials: SY
authors.lastname: Yi
authors.forename: In Jin
authors.initials: IJ
authors.lastname: Jang
authors.forename: Sang Goo
authors.initials: SG
authors.lastname: Shin
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood*; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000970:Antineoplastic Agents; C116963:belotecan; D002166:Camptothecin