Evaluating the in vitro adhesive strength of biomaterials. Biosimulator for selective leak closure.
OBJECTIVE
Surgical measures always involve problems of hemostasis and tissue management. Tissue sealing can be carried out in this case, with knowledge of the adhesive strength of liquid and fleece-bound sealing being of great clinical relevance. By employing a leak closure model, it has been possible to perform in vitro standardized technological-biomechanical experiments on a biomembrane mounted in a pressure chamber (biosimulator).


RESULTS
Liquid sealing (fibrin gluing: 4.1 hPa, photopolymerisate: 82.9 hPa) offers minimal and maximal adhesive strengths. Fleece-bound sealing does not depend on the carrier material and is always more stabile than pure liquid sealing, whereby systems that must be coated "on the spot" (prepare-to-use: 22.3-25.3 hPa) exhibit significantly lower adhesive strength (p < 0.0001) than a biodegradable collagen system, which is ready-to-use thanks to its fibrinogen-based coating (TachoComb H). Practicability, effectiveness and efficiency are further advantages of ready-to-use systems.


CONCLUSIONS
The biosimulator, presented here, is flexible in terms of its application for tissue management and it enables reproducible, economic and ecological evaluation of repair systems, e.g. tissue sealings.
Carbon
Roman T
RT
Department of Paediatric Surgery, Friedrich-Alexander-University Erlangen-Nuremberg, Maximiliansplatz 2, 91054 Erlangen, Germany. roman.carbon@chir.imed.uni-erlangen.de
Baar
Sibylle
S
Kriegelstein
Stefanie
S
Huemmer
Hans P
HP
Baar
Knut
K
Simon
Stefanie-Isabell
SI
D000818:Animals; D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D003094:Collagen; D015718:Fibrin Tissue Adhesive; D006488:Hemostasis, Surgical; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing / Q000295:instrumentation*; D008954:Models, Biological; D010994:Pleura; D011312:Pressure; D034421:Sus scrofa; D014014:Tissue Adhesives
D001672:Biocompatible Materials; D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives; D003094:Collagen