Bovine milk enhances the oxidative burst activity of polymorphonuclear leukocytes in low concentrations.
Bovine milk contains various immunoreactive components, and the activation of polymorphonuclear leukocytes (PMNLs) function in breast-fed infants has been reported. In this study, the effect of milk on the oxidative burst of bovine PMNLs was investigated in vitro. When PMNLs were incubated with 0.1% colostrum or normal milk, the oxidative burst induced by serum-opsonized Staphylococcus aureus was enhanced, and the enhancement declined dose-dependently. The enhancement of the oxidative burst by milk was not due to opsonins but the priming activities. Also, the phorbol 12-myristate 13-acetate (PMA)-induced oxidative burst increased after incubation with 0.1% colostrum, but the colostral enhancement of the oxidative burst was unaffected by the incubation time. These results suggest that bovine milk contains oxidative burst promoting factor(s).
Sugisawa
Hitoshi
H
Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan.
Itou
Takuya
T
Ichimura
Yuri
Y
Sakai
Takeo
T
D000818:Animals; D002417:Cattle; D003126:Colostrum / Q000276:immunology; D008892:Milk / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D016897:Respiratory Burst; D013211:Staphylococcus aureus / Q000276:immunology; D013997:Time Factors