[Effect of hyperthermia on tight junctions between endothelial cells of the blood-brain barrier model in vitro].
OBJECTIVE
To investigate the effect of hyperthermia in vitro on tight junctions between the endothelial cells of the blood-brain barrier.


METHODS
An in vitro blood-brain barrier model was established by coculture of ECV304 cells with astrocytes. Transendothelial resistance (TER) of in vitro blood-brain barrier was determined by Millicell-ERS system, and the morphological change of tight junctions examined by silver staining. The expression level of zonula occluden-1(ZO-1) was analyzed by semi-quantitative reverse transcriptase-PCR.


RESULTS
The integrity of tight junctions was disrupted and the expression level of ZO-1 decreased after treatment with hyperthermia.


CONCLUSIONS
Tight junctions between the endothelial cells of the blood-brain barrier can be destroyed by hyperthermia, and decreased expression level of ZO-1 induced by hyperthermia is one of the most important molecular mechanisms.
Chen
Yi-zhao
YZ
Department of Neurosurgery, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Xu
Ru-xiang
RX
Huang
Qi-jin
QJ
Xu
Zhong-jun
ZJ
Jiang
Xiao-dan
XD
Cai
Ying-qian
YQ
D000818:Animals; D001253:Astrocytes / Q000502:physiology; D001812:Blood-Brain Barrier; D018920:Coculture Techniques; D004730:Endothelium, Vascular / Q000378:metabolism / Q000648:ultrastructure*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006358:Hot Temperature; D008565:Membrane Proteins / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D019108:Tight Junctions / Q000378:metabolism / Q000648:ultrastructure*; D062826:Zonula Occludens-1 Protein
D005904:Glial Fibrillary Acidic Protein; D008565:Membrane Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; C569044:Tjp1 protein, rat; D062826:Zonula Occludens-1 Protein