Semliki forest virus-based expression for versatile use in receptor research.
Semliki Forest virus (SFV) vectors have been generated for highly efficient studies on gene expression in a variety of mammalian host cells, including immortalized cell lines as well as primary cells in culture. Moreover, SFV expression has been scaled up for mammalian suspension cultures in spinner flasks and bioreactors for production of large quantities of recombinant proteins for drug screening and purification. The strong preference of expression in neuronal cells in primary cell cultures, in organotypic hippocampal slices and in vivo has made SFV vectors attractive for neurobiological studies. Additionally, the engineering of novel, less cytotoxic and temperature-sensitive SFV mutant vectors has further increased their application range.
Lundstrom
Kenneth
K
Regulon Inc., Biopole Epalinges, Les Croisettes 22, CH-1066 Epalinges/Lausanne, Switzerland. kenneth@regulon.org
D000818:Animals; D035843:Biomedical Research; D015870:Gene Expression; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D011956:Receptors, Cell Surface / Q000502:physiology*; D012672:Semliki forest virus / Q000235:genetics*; D014162:Transfection
D011956:Receptors, Cell Surface