Simian virus 40 sequences in human lymphoblastoid B-cell lines.
Human Epstein-Barr virus-immortalized lymphoblastoid B-cell lines tested positive by PCR for simian virus 40 (SV40) DNA (22 of 42 cell lines, 52.3%). B lymphocytes or tissues from which B-cell lines derived were also SV40 positive. In situ hybridization showed that SV40 DNA was present in the nucleus of a small fraction (1/250) of cells. SV40 T-antigen mRNA was detected by reverse transcription-PCR. Lymphoblastoid B-cell lines (n = 4) infected with SV40 remained SV40 positive for 4 to 6 months. SV40-positive B-cell lines were more tumorigenic in SCID mice than were SV40-negative cell lines (4 of 5 [80%] SV40-positive cell lines versus 2 of 4 [50%] SV40-negative cell lines). These results suggest that SV40 may play a role in the early phases of human lymphomagenesis.
Dolcetti
Riccardo
R
Divisions of Experimental Oncology, Centro di Riferimento Oncologico, IRCCS, 33081 Aviano (Pordenone), Italy.
Martini
Fernanda
F
Quaia
Michele
M
Gloghini
Annunziata
A
Vignocchi
Beatrice
B
Cariati
Roberta
R
Martinelli
Marcella
M
Carbone
Antonino
A
Boiocchi
Mauro
M
Tognon
Mauro
M
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D001402:B-Lymphocytes / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013539:Simian virus 40 / Q000235:genetics / Q000302:isolation & purification*
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D012333:RNA, Messenger