title: Enhanced immunogenicity to Mycobacterium tuberculosis by vaccination with an alphavirus plasmid replicon expressing antigen 85A.
abstract: The immunogenicity of a plasmid DNA vaccine incorporating Sindbis virus RNA replicase functions (pSINCP) and expressing antigen 85A (Ag85A) from Mycobacterium tuberculosis was compared with a conventional plasmid DNA vector encoding Ag85A. pSINCP-85A was highly immunogenic in mice and gave enhanced long-term protection against M. tuberculosis compared with the conventional vector.
authors.affiliation: Cellular Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, and Food and Drug Administration, Bethesda, Maryland 20892, USA.
authors.forename: Joanna R
authors.initials: JR
authors.lastname: Kirman
authors.forename: Tara
authors.initials: T
authors.lastname: Turon
authors.forename: Hua
authors.initials: H
authors.lastname: Su
authors.forename: Amy
authors.initials: A
authors.lastname: Li
authors.forename: Carl
authors.initials: C
authors.lastname: Kraus
authors.forename: John M
authors.initials: JM
authors.lastname: Polo
authors.forename: John
authors.initials: J
authors.lastname: Belisle
authors.forename: Sheldon
authors.initials: S
authors.lastname: Morris
authors.forename: Robert A
authors.initials: RA
authors.lastname: Seder
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000276:immunology; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D012093:Replicon / Q000235:genetics / Q000276:immunology; D012845:Sindbis Virus / Q000235:genetics / Q000276:immunology; D032581:Tuberculosis Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000517:prevention & control*; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D032581:Tuberculosis Vaccines; D019444:Vaccines, DNA; D007371:Interferon-gamma; D000217:Acyltransferases; C112580:antigen 85A, Mycobacterium tuberculosis; D012324:RNA-Dependent RNA Polymerase