title: Plectin-like proteins are present in cells of Chlamydomonas eugametos (Volvocales).
abstract: Using both monoclonal and polyclonal antibodies against mammalian plectin (multifunctional protein cross-linking cytoskeletal structures, mainly intermediate filaments, in mammalian cells), several putative isoforms of plectin-like proteins were found in protein extracts from the green alga Chlamydomonas eugametos (Volvocales). Immunofluorescence and immunoblotting revealed that some of the plectin-like proteins were present in perinuclear region or localized near the cell wall, probably being attached to the cytoplasmic membrane.
authors.affiliation: Division of Autotrophic Microorganisms, Institute of Microbiology, Academy of Sciences of the Czech Republic, 379 81 Trebon.
authors.forename: J
authors.initials: J
authors.lastname: Hendrychová
authors.forename: M
authors.initials: M
authors.lastname: Vítová
authors.forename: K
authors.initials: K
authors.lastname: Bisová
authors.forename: G
authors.initials: G
authors.lastname: Wiche
authors.forename: V
authors.initials: V
authors.lastname: Zachleder
mesh_terms: D020418:Algal Proteins / Q000378:metabolism*; D000818:Animals; D002696:Chlamydomonas / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D051190:Plectin; D011817:Rabbits; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D020418:Algal Proteins; D007381:Intermediate Filament Proteins; D051190:Plectin