The foot-and-mouth disease virus cis-acting replication element (cre) can be complemented in trans within infected cells.
A temperature-sensitive (ts) mutation was identified within the 5'-untranslated region of foot-and-mouth disease virus (FMDV) RNA. The mutation destabilizes a stem-loop structure recently identified as a cis-acting replication element (cre). Genetic analyses indicated that the ts defect in virus replication could be complemented. Thus, the FMDV cre can function in trans. It is suggested that the cre be renamed a 3B-uridylylation site (bus).
Tiley
Laurence
L
Institute for Animal Health, Pirbright, Woking, Surrey GU24 ONF, United Kingdom.
King
Andrew M Q
AM
Belsham
Graham J
GJ
D020121:5' Untranslated Regions / Q000737:chemistry*; D000818:Animals; D006224:Cricetinae; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics*; D005816:Genetic Complementation Test; D009154:Mutation; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry*; D012045:Regulatory Sequences, Nucleic Acid; D014779:Virus Replication
D020121:5' Untranslated Regions; D012367:RNA, Viral