Strategies and outcomes of the first 100 cycles of preimplantation genetic diagnosis at the Guy's and St. Thomas' Center.
OBJECTIVE
To establish strategies for the implementation of a successful preimplantation genetic diagnosis (PGD) service.


METHODS
Retrospective review of data from a single center.


METHODS
A United Kingdom National Health Service hospital.


METHODS
Patients (60 couples) were referred for PGD from UK genetic centers.


METHODS
We followed the protocol of ovarian stimulation, oocyte retrieval, fertilization, single cell biopsy on day 3, and embryo transfer on day 4. Pregnancies unaffected by the familial genetic condition.


RESULTS
A total of 60 couples was treated for 20 different conditions. Early cycles using nonsequential embryo culture media were less successful (13% pregnancy rate/embryo transfer) than later cycles using sequential media (33.5%). Ninety-four percent of embryos (n = 473) had a single cell removed at biopsy. The overall pregnancy rate was 24% per cycle started, 29% per egg collection, 38% per transfer, and 40% per couple treated. In one cycle, an affected pregnancy followed PGD for spinal muscular atrophy (SMA).


CONCLUSIONS
The use of sequential media and single cell biopsy results in a successful PGD program with encouraging pregnancy rates.
Pickering
Susan
S
Center for Preimplantation Genetic Diagnosis, Guy's and St. Thomas' NHS Trust, Guy's Hospital, London Bridge, London, United Kingdom. susan.pickering@kcl.ac.uk
Polidoropoulos
Nikolaos
N
Caller
Jenny
J
Scriven
Paul
P
Ogilvie
Caroline Mackie
CM
Braude
Peter
P
D002869:Chromosome Aberrations; D015925:Cryopreservation; D046508:Culture Techniques; D004624:Embryo Transfer; D004622:Embryo, Mammalian; D005260:Female; D005307:Fertilization in Vitro; D030342:Genetic Diseases, Inborn / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D008297:Male; D010062:Ovulation Induction; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011256:Pregnancy Outcome; D019836:Preimplantation Diagnosis; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D016896:Treatment Outcome; D006113:United Kingdom