Accuracy of a commercial enzyme-linked immunosorbent assay for CagA in patients from Brazil with and without gastric carcinoma.
We validated a commercial enzyme-linked immunosorbent assay for the detection of anti-CagA antibodies in Brazilian patients with Helicobacter pylori infection. The test presented high sensitivity (97.4%) and specificity (88.9%) when employed in patients without gastric carcinoma. However, in gastric carcinoma patients, the test was neither sensitive nor specific enough to detect cagA-positive H. pylori infection.
Rocha
Andreia Maria Camargos
AM
Laboratory of Research in Bacteriology, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Rocha
Gifone Aguiar
GA
Santos
Adriana
A
de Oliveira
Celso Affonso
CA
Queiroz
Dulciene Maria Magalhães
DM
D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis*; D001938:Brazil; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D016481:Helicobacter Infections / Q000150:complications / Q000276:immunology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D011933:Reagent Kits, Diagnostic; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000150:complications / Q000382:microbiology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011933:Reagent Kits, Diagnostic; C080866:cagA protein, Helicobacter pylori