Chemoattraction of human T cells by IL-18.
Cell locomotion is crucial to the induction of an effective immune response. We report here the chemoattraction of CD4(+) T cells by IL-18, a member of the IL-1 cytokine family. Recombinant IL-18 increased the proportion of T cells in polarized morphology in vitro and stimulated their subsequent invasion into collagen gels in an IL-18 concentration gradient-dependent manner. Immunofluorescent microscopy studies determined that the major cell type responding to IL-18 was IL-18R(+)CD4(+). Importantly, synovial CD4(+) T cells from patients with rheumatoid arthritis responded to IL-18, adopting polarized morphology and gel invasion without further activation ex vivo, indicating the physiologic relevance of our observations. Finally, injection of rIL-18 into the footpad of DBA/1 mice led to local accumulation of inflammatory cells. These data therefore demonstrate for the first time lymphocyte chemoattractant properties of a member of the IL-1 cytokine family and its relevance in inflammatory diseases.
Komai-Koma
Mousa
M
Division of Immunology, Infection and Inflammation, Faculty of Medicine, University of Glasgow, United Kingdom.
Gracie
J Alastair
JA
Wei
Xiao-Qing
XQ
Xu
Damo
D
Thomson
Neil
N
McInnes
Iain B
IB
Liew
Foo Y
FY
D000818:Animals; D048429:Cell Size / Q000276:immunology; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D007279:Injections, Subcutaneous; D020382:Interleukin-18 / Q000008:administration & dosage / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation; D016131:Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D051379:Mice; D008811:Mice, Inbred DBA; D013583:Synovial Membrane / Q000166:cytology / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology*; D018417:Th1 Cells / Q000166:cytology / Q000276:immunology
D020382:Interleukin-18