[Inhibition of mixture of lucid garoderma and lucid garoderma spore on tumor cell in vitro and in vivo].
OBJECTIVE
Garoderma lucidum (lingzhi) is a kind of medicinal fungus with a long history in China as a valuable tonic folk remedy. It has been demonstrated that lingzhi had growth inhibition effect against implanted mice tumors. It is generally regarded that anti-tumor action of lingzhi is induced by promoting function of immune system. Recently, we found that mixture of lucid garoderma and lucid garoderma spore (MLGLGS) could markedly inhibit activities of topoisomerase I and II, so this study was designed to further evaluate the inhibition effects of MLGLGS on tumor cell in vitro and in vivo.


METHODS
Inhibition of MLGLGS on tumor cell were detected by MTT or SRB method in vitro study and by human tumor nude mice xenografts as models in vivo study.


RESULTS
Among them, the IC50 values were lower than 2 mg/ml in P388, U-937, and HL-60 leukemias, and lower than 4 mg/ml in 2 human lung cancer and 2 human gastric cancer cells, respectively. In vivo study revealed that MLGLGS had certain experimental therapeutic action on human lung tumor xenograft LAX-83 (dose: 1 g/kg, inhibitory rate: 49.47%) and gastric xenograft SGC-7901 (dose: 2 g/kg, inhibitory rate: 43.09%).


CONCLUSIONS
MLGLGS has inhibitory action on tumor cell at high concentration and high dosage.
Chen
Ling-ji
LJ
Division of Anti-Tumor Pharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, P. R. China.
Han
Jia-xian
JX
Yang
Wei-yi
WY
Lu
Li-juan
LJ
Zhang
Jia-liu
JL
Yang
Qiu-long
QL
Yuan
Sheng-tao
ST
Ding
Jian
J
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D018922:HL-60 Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000188:drug therapy*; D010936:Plant Extracts / Q000627:therapeutic use; D020940:Reishi; D013172:Spores, Fungal / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D020298:U937 Cells; D023041:Xenograft Model Antitumor Assays
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts