Effects of multipurpose solutions (MPS) for hydrogel contact lenses on gap-junctional intercellular communication (GJIC) in rabbit corneal keratocytes.
To ensure the effects of multipurpose solutions (MPS) for hydrogel contact lenses on the cornea, the inhibitory activity of three types of MPS on corneal cells has been evaluated with the use of scrape loading and dye transfer assay (SLDT assay) and Western blotting on rabbit corneal keratocytes (RC4). In SLDT assay, MPS-A and poloxamine showed dose-dependent inhibitory activity, suggesting the inhibitory action of MPS-A and poloxamine to gap junctional intercellular communication (GJIC) in the tested cells. Moreover, after treatment with MPS-A, the GJIC was initially inhibited within 4 h, and thereafter gradually turned to an approximately 60% level of the initial value. When MPS-A was removed from the incubation media after exposure of the cells, the recovery of GJIC was time dependent and returned to approximately initial levels at 8 h. Complete recovery was established after approximately 24 h. These findings suggested that the inhibitory action of MPS-A on corneal keratocytes was reversible. This inhibition was accompanied by a decrease in the quantity of connexin-43, which is a major protein constituting the gap junctional channel of these cells, and its change in the phosphorylation state. Taken together, it was suggested that MPS-A interacts with connexin-43, inducing an inhibitory action on GJIC.
Sumide
Taizo
T
Division of Medical Devices, National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Tsuchiya
Toshie
T
D000818:Animals; D041702:BALB 3T3 Cells; D002450:Cell Communication / Q000187:drug effects; D018031:Connexin 43 / Q000378:metabolism; D018639:Contact Lens Solutions / Q000737:chemistry / Q000633:toxicity*; D003263:Contact Lenses, Hydrophilic / Q000009:adverse effects; D003315:Cornea / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D017629:Gap Junctions / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007546:Isoquinolines / Q000493:pharmacokinetics; D008422:Materials Testing; D051379:Mice; D023421:Models, Animal; D011817:Rabbits; D012449:Safety
D018031:Connexin 43; D018639:Contact Lens Solutions; D005456:Fluorescent Dyes; D007546:Isoquinolines; C017475:lucifer yellow