title: Failure of angiotensin II and insulin to stimulate transforming growth factor-beta1. Release from cultured bovine retinal pericytes.
abstract: BACKGROUND
  Activation of the renin-angiotensin system (RAS) may induce cardiovascular and renal fibrosis in hypertension and diabetes. This fibrogenic effect is mainly mediated by Transforming Growth Factor-B1 (TGF-B1), a multifunctional citokyne released by endothelial, vascular smooth muscle and renal mesangial cells, that is able to increase extracellular matrix deposition. Retinal capillary pericytes have functions similar to those of mesangial cells, including ability to synthesize and release TGF-B1 and produce extracellular matrix. An intraocular RAS was described in the human eye and may produce effects similar to those observed in the heart and kidney, which could be mediated by TGF-B1. In particular, TGF-B1 might be involved in thickening of the capillary basement membrane in diabetic microangiopathy. We therefore aimed at evaluating the possible effects of Angiotensin-II on TGF-B1 secretion by cultured retinal pericytes (BRP).
  
  
  METHODS
  BRP cultures were incubated with Angiotensin-II or insulin (known to play a permissive effect on TGF-B1 release from mesangial cells) or Angiotensin-II + insulin at final concentrations of 10-10, 10-8, 10-6, 10-4 mol/L.
  
  
  RESULTS
  Baseline TGF-B1 concentrations in the supernatants of pericyte cultures were 6 139 +/- 1 919 pg/mL/106 cells; no changes of TGF-B1 concentrations resulted from adding increasing amounts of Ang II, insulin or both.
  
  
  CONCLUSIONS
  Though confirming that cultured bovine retinal pericytes spontaneously release TGF-B1, Angiotensin-II did not produce any stimulatory effects of in our experimental system
authors.affiliation: Department of Internal Medicine, University of Torino, Italy. paolopiero.limone@unito.it
authors.forename: P
authors.initials: P
authors.lastname: Limone
authors.forename: C
authors.initials: C
authors.lastname: Berardi
authors.forename: F
authors.initials: F
authors.lastname: Pomero
authors.forename: P
authors.initials: P
authors.lastname: Del Rizzo
authors.forename: A
authors.initials: A
authors.lastname: Allione
authors.forename: E
authors.initials: E
authors.lastname: Beltramo
authors.forename: F
authors.initials: F
authors.lastname: D'alu
authors.forename: E
authors.initials: E
authors.lastname: Ponte
authors.forename: C
authors.initials: C
authors.lastname: Pellissetto
authors.forename: A
authors.initials: A
authors.lastname: Lacaria
authors.forename: A M
authors.initials: AM
authors.lastname: Barberis
authors.forename: M
authors.initials: M
authors.lastname: Porta
mesh_terms: D000704:Analysis of Variance; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D007328:Insulin / Q000494:pharmacology*; D020286:Pericytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012160:Retina / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D053773:Transforming Growth Factor beta1
chemical_list: D007328:Insulin; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D000804:Angiotensin II