title: Aquaporin expression in developing human teeth and selected orofacial tissues.
abstract: The aquaporin (AQP) family of membrane channel proteins function as selective pores through which water, glycerol, and other small solutes cross the cell plasma membrane. To date, 11 members of this transporter family, designated AQP0-10, have been cloned and characterized in humans. The AQPs are differentially expressed in temporospatial patterns, where different AQPs demonstrate distinct tissue distributions that may reflect differing cell membrane transport functions. The purpose of this study was to evaluate AQP expression in the developing human teeth by RT-PCR and Western blot analysis. To access the generality of AQP expression, selected other orofacial tissues were studied by RT-PCR. The presence of all eleven human AQPs was screened in each tissue by RT-PCR. Positive amplification products were verified by direct DNA sequencing. AQPs 1, 3, 4, 5, 6, and 10 were identified by RT-PCR in developing teeth, and AQP1, 3, 5, and 6 were confirmed by Western blot analysis. AQP 4 was not detected by Western blot analysis, and we were unable to test for the recently identified AQP10 due to unavailability of antibodies. AQPs detected in other orofacial tissues by RT-PCR included gingiva (AQP3, 7, 10); Meckel's cartilage (AQP1, 3, 4, 5, 6); submandibular gland (AQP1, 3, 4, 5, 6, 7); masseter muscle (AQP1, 3, 4, 7, 8, 9,10); and infrahyoid muscle (AQP1, 3, 4,10). These results demonstrate that multiple aquaporins are expressed in developing teeth and in selected orofacial tissues.
authors.affiliation: Center for Craniofacial and Dental Genetics, Division of Oral Biology, School of Dental Medicine, University of Pittsburgh, 614 Salk Hall, 3501 Terrace Street, Pittsburgh, PA 15261, USA.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: P S
authors.initials: PS
authors.lastname: Hart
authors.forename: N P
authors.initials: NP
authors.lastname: Piesco
authors.forename: X
authors.initials: X
authors.lastname: Lu
authors.forename: M C
authors.initials: MC
authors.lastname: Gorry
authors.forename: T C
authors.initials: TC
authors.lastname: Hart
mesh_terms: D000293:Adolescent; D000328:Adult; D020346:Aquaporins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002358:Cartilage, Articular / Q000254:growth & development / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D005881:Gingiva / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D008297:Male; D008406:Masseter Muscle / Q000254:growth & development / Q000378:metabolism; D008875:Middle Aged; D009055:Mouth; D038081:Organogenesis; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013284:Stomatognathic System; D013363:Submandibular Gland / Q000254:growth & development / Q000378:metabolism; D014070:Tooth / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D014083:Tooth Germ / Q000196:embryology / Q000378:metabolism
chemical_list: D020346:Aquaporins; D017931:DNA Primers; D012333:RNA, Messenger; D012313:RNA