title: Molecular characterization of the cDNA and localization of the mRNA encoding the prohormone convertase PC5-A in the European green frog.
abstract: The structure and distribution of PC5-A, a prohormone convertase that is thought to be involved in post-translational processing of peptide hormone and neuropeptide precursors, have not been investigated in submammalian vertebrates. In the present study, we characterized the cDNA encoding PC5-A in the European green frog Rana esculenta. The frog PC5-A cDNA encodes a 913-amino acid protein that encompasses a 28-amino acid signal peptide, the Asp/His/Ser catalytic triad found in all serine proteinases of the subtilisin family, and two potential N-linked glycosylation sites located in a C-terminal cysteine-rich domain. Reverse transcriptase polymerase chain reaction amplification showed that PC5-A mRNA is expressed in various organs including the brain, spinal cord, pituitary, lung, liver, intestine, and testis, but not in the stomach and pancreas. The distribution of PC5-A mRNA in the frog brain was studied by in situ hybridization histochemistry. Intense expression was observed in the mitral cellular layer of the olfactory bulb, the nucleus of the diagonal band of Broca, the anterior preoptic area, and the suprachiasmatic and ventral hypothalamic nuclei. The expression pattern of PC5-A mRNA in the central nervous system of anuran amphibians was consistent with the implication of this prohormone convertase in the processing of various neuropeptide precursors.
authors.affiliation: European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U-413, UA CNRS, University of Rouen, 76821 Mont-Saint-Aignan, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Gangnon
authors.forename: Sylvie
authors.initials: S
authors.lastname: Jégou
authors.forename: Mauro
authors.initials: M
authors.lastname: Vallarino
authors.forename: Didier
authors.initials: D
authors.lastname: Vieau
authors.forename: Hubert
authors.initials: H
authors.lastname: Vaudry
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D018076:DNA, Complementary / Q000737:chemistry*; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000737:chemistry*; D045702:Proprotein Convertase 5; D012333:RNA, Messenger / Q000032:analysis*; D011893:Rana esculenta / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D045702:Proprotein Convertase 5; D012697:Serine Endopeptidases