[Detection of rotavirus RNA using DIG labelled probe prepared by polymerase chain reaction].
The digoxigenin (DIG) labelled cDNA probe of rotavirus was directly prepared by polymerase chain reaction (PCR). The results of cDNA-RNA hybridization showed that the DIG-cDNA probe exhibits rotavirus specificity and can detect as tiny as 10 pg rotavirus RNA. 120 fecal samples of diarrhea from infants and young children were tested by dot-blot hybridization. It was shown that the positive rate of dot-blot hybridization(65.0%) was significantly higher than that of PAGE(49.1%). This study indicated that direct preparation of DIG-labelled rotavirus-cDNA probe by PCR is much faster and simpler than common method of labeling.
Fu
W
W
Department of Pediatrics of Zhujiang Hospital, First Military University, Guangzhou 510282.
Hao
W
W
Peng
Y
Y
D000328:Adult; D018076:DNA, Complementary; D004076:Digoxigenin; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007223:Infant; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000821:virology*
D018076:DNA, Complementary; D012367:RNA, Viral; D004076:Digoxigenin