[Effect of oxidatively modified high density lipoprotein on transcriptional expression of proto c-fos and PDGF receptor genes in cultured human arterial smooth muscle cell].
The proliferation of arterial smooth muscle cell (SMC) plays an important role in the process of atherosclero-genesis. Using the establishment of primary culture and sub-culture method for human arterial SMC, we observed the effects of native HDL (N-HDL) and oxidized HDL of proto c-fos and PDGF receptor genes transcriptional expression in cultured SMC. The results showed that N-HDL had no effect on the expression of proto c-fos and PDGF receptor genes in the cultured human SMC, that OX-HDL had no effect on the expression of PDGF receptor gene in the cultured human SMC, and that OX-HDL promoted the expression of protoc-fos gene significantly (P < 0.01). These results suggest that the OX-HDL's atherogenic role is closely related to its stimulating effect on proto c-fos gene expression of SMC.
Jiang
Y
Y
Institute of Biochemistry and Molecular Biology, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
Liu
B
B
Qin
Y
Y
Sun
Z
Z
D001011:Aorta / Q000166:cytology; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D008075:Lipoproteins, HDL / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D010084:Oxidation-Reduction; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D017479:Receptors, Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000235:genetics
D008075:Lipoproteins, HDL; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D017479:Receptors, Platelet-Derived Growth Factor