Platelet activation and PDGF-AB release during dialysis.
During hemodialysis the blood-membrane contact causes a release of platelet granule content, which contains Platelet Derived Growth Factor (PDGF-AB). In view of its possible role in accelerated atherosclerotic processes, we evaluated the intra- and post-dialytic changes in PDGF-AB serum levels during hemodialysis sessions performed with Hemophan and Polysulfone membranes. PDGF-AB, PF4, betaTG and MPV levels were determined in the peripheral blood in 30 patients each of whom underwent 6 dialysis sessions: 3 with Hemophan (HE) membrane and 3 with Polysulfone (PS) membrane, interpolated by a wash out session with PS membrane. Blood samples were taken at times 0', 30', 120', 180', 240' during dialysis sessions and at 1, 4 and 20 hours after the end of the session. Statistical analysis was done using the ANOVA one way test and Student's t test PDGF-AB serum levels initially increased and, except for a sharp fall at 120', remained constantly high during HD with both membranes tested, not returning to basal values until 20 hours after the end of the session. PF4, betaTG and MPV all showed a similar trend to PDGF. No statistically significant difference was found between the two membranes tested. PDGF-AB, a powerful growth factor in cells of mesenchymal origin, is released during dialysis mainly as a result of the blood-membrane contact. This we found regardless of the type of dialyzer we tested, and, above all, proved to return very slowly to basal values. We speculate that the release of PDGF-AB could play a part like other atherosclerosis risk-factors in the appearance and worsening of atherosclerotic lesions in hemodialysis patients.
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Nephrology Dialysis and Renal Transplantation Unit, Department of Clinical Medicine and Applied Biotechnology, S. Orsola University Hospital, Bologna, Italy. emodia@orsola-malpighi.med.unibo.it
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La Manna
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Feliciangeli
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Stefoni
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D000368:Aged; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D016014:Linear Models; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D015539:Platelet Activation / Q000502:physiology*; D010976:Platelet Count; D010978:Platelet Factor 4 / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D006435:Renal Dialysis / Q000295:instrumentation*; D001620:beta-Thromboglobulin / Q000378:metabolism
D008567:Membranes, Artificial; D010982:Platelet-Derived Growth Factor; D001620:beta-Thromboglobulin; C095477:platelet-derived growth factor AB; D010978:Platelet Factor 4