title: Cytokines in mammary lymph and milk during endotoxin-induced bovine mastitis.
abstract: Cytokine kinetics were examined in milk and in afferent and efferent lymph of the supramammary lymph node after intramammary infusion of endotoxin from Escherichia coli. Cows were sampled 0, 2 and 4h after infusion (p.i.). Neutrophils appeared in afferent lymph 2h p.i., and in efferent lymph and milk 4h p.i. The milk contained high concentrations of interleukin-8 (IL-8) at 2 and 4h p.i. IL-8 was also found in lymph, but at lower concentrations. The tumor necrosis factor-alpha (TNF-alpha) concentration tended to increase in afferent lymph at 2h p.i., and increased in milk at 4h p.i. The level of IL-1beta increased at 4h p.i. in milk, but was not detected in lymph. Interferon-gamma was not detected in any sample, at any time. The results indicate a primary role for IL-8 in the recruitment of neutrophils into the gland, and suggest that IL-1beta and TNF-alpha are not necessary for IL-8 production and release in response to endotoxin.
authors.affiliation: Department of Ruminant and Porcine Diseases, National Veterinary Institute, SE-751 89, Uppsala, Sweden. Karin.Persson.Waller@sva.se
authors.forename: K
authors.initials: K
authors.lastname: Persson Waller
authors.forename: I G
authors.initials: IG
authors.lastname: Colditz
authors.forename: S
authors.initials: S
authors.lastname: Lun
authors.forename: K
authors.initials: K
authors.lastname: Ostensson
mesh_terms: D000818:Animals; D002417:Cattle; D016207:Cytokines / Q000032:analysis*; D004731:Endotoxins / Q000494:pharmacology*; D005260:Female; D007375:Interleukin-1 / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis; D007958:Leukocyte Count; D008196:Lymph / Q000737:chemistry*; D008414:Mastitis, Bovine / Q000139:chemically induced* / Q000378:metabolism*; D008892:Milk / Q000737:chemistry*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D016207:Cytokines; D004731:Endotoxins; D007375:Interleukin-1; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha