MG63 osteoblastic cell adhesion to the hydrophobic surface precoated with recombinant osteopontin fragments.
The hydrophobicity of biomaterials has been recognized as a limitation to the adequate function of anchorage-dependent cells when hydrophobic biomaterials are used for tissue engineering. This is due to flawed solid-state signals from cell adhesion. In this study, a recombinant osteopontin (rOPN17-169) fragment containing the cell adhesion motifs was expressed in E. coli and was precoated on the hydrophobic surface prior to osteoblastic MG63 cell culture. Precoating the hydrophobic surface with rOPN17-169 improved osteoblastic cell adhesion, which was blocked by soluble RGDS. The adhesion of MG63 cells to rOPN17-169 pre-coated surface-activated mitogen-activated protein kinases (MAPK) such as extracellular signal-receptor kinase 1/2, p38, and c-Jun N-terminal kinase (JNK). In addition, p38 MAPK was activated in response to a soluble factor of transforming growth factor-beta in the cells adhered to the hydrophobic surface via rOPN17-169. This suggests that rOPN17-169 precoated on the hydrophobic surface can allow osteoblastic cells to generate adhesion signals sufficient for cell adhesion, MAPK activation, and the cytokine activation of osteoblastic cells.
Lee
Yun Jung
YJ
Laboratory for the Study of Molecular Biointerfaces, Department of Oral Anatomy, College of Dentistry, BK21HLS, and Intellectual Biointerface Engineering Center, Seoul National University, 28-22, YeonKun-Dong, ChongRo-Ku, Seoul 110-749, South Korea.
Park
Su Jin
SJ
Lee
Woo Kul
WK
Ko
Jea Seung
JS
Kim
Hyun Man
HM
D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D003001:Cloning, Molecular; D020099:Coated Materials, Biocompatible; D017931:DNA Primers; D004926:Escherichia coli; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000502:physiology; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D053495:Osteopontin; D010446:Peptide Fragments / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000494:pharmacology; D012795:Sialoglycoproteins / Q000235:genetics / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases
D020099:Coated Materials, Biocompatible; D017931:DNA Primers; D010446:Peptide Fragments; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D053495:Osteopontin; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases