[The inhibitory effect of antisense--RNA of papillomavirus type 16 E6E7 genes on malignant phenotypes of cervical carcinoma cell line].
In our previous study, the malignant phenotypes of the NIH3T3 cells, transformed with HPV-16 E6E7 genes, could be inhibited by the antisense RNA of HPV-16 E6E7. In this study, the antisense RNA of HPV-16 E6E7 was induced into the cervical carcinoma cells, caski, which contained HPV-16 DNA. The results showed that the cellular growth velocity was down regulated by the antisense RNA of HPV-16 E6E7, the ability of forming cellular colonies in soft agar medium and the tumorigenicity in nude mice of CaSki cells were also inhibited by the antisense cellular colonies in soft agar median and the tumorigenicity in nude mice of Caski cells were also inhibited by the antisense RNA. In addition, the decrease of the expression of viral early genes in carcinoma cells was detected with Western blot assay. It denoted that the phenotypes and the carcinogenicity of tumor cells were inhibited by the associated antisense RNA, and this may be one of the pathways inducing tumor cells reversion.
Ming
L
L
Cancer Institute, Chinese Academy Medical Science, Peking Union Medical College, Beijing 100021.
Zhou
L
L
Shang
M
M
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics; D050725:Papillomavirus E7 Proteins; D010641:Phenotype; D016372:RNA, Antisense / Q000494:pharmacology*; D012097:Repressor Proteins; D014157:Transcription Factors / Q000235:genetics; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology / Q000821:virology*
D004279:DNA, Viral; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D016372:RNA, Antisense; D012097:Repressor Proteins; D014157:Transcription Factors; C059731:oncogene protein E7, Human papillomavirus type 16