[Expression of novel cloned genes HC56, HC71 and HC90 mapped on human chromosome 17p13.3 in leukemic cells].
In order to investigate expression of novel genes HC56, HC70 and HC90 mapped on chromosome 17p13.3 in human leukemic cells, HC56, HC71 and HC90 genes expression was examined in cells from 35 patients with acute leukemias and 4 leukemic cell lines by using semi-quantitative RT-PCR with incorporation of alpha(32)P dATP, betaM2 gene as endogenous control. The results showed that HC90 gene expression level was lower in patients with acute lymphocytic leukemia and T lymphocytic leukemic cell line Jurkat than that in normal control. Low-level HC71 gene expression was detected in acute myeloid leukemia. There was no expression difference in HC56 between leukemic cells and normal blood cells. It was concluded that both HC70 and HC90 genes were aberrantly expressed in human leukemic cells.
Wang
Rong
R
Institute of Hematology, Medical College of Jinan University, Guangzhou 510632, China. rwang@iarc.fr
Po
Xi-Yan
XY
Wan
Da-Fang
DF
Yu
Zhi
Z
Du
Jun-Xiang
JX
Mu
Qi-Tian
QT
Zhang
Yuan
Y
Gu
Jin-Ren
JR
D000208:Acute Disease; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction