[Tenotoxic potential of fluoroquinolones in the choice of surgical antibiotic prophylaxis in ophthalmology].
OBJECTIVE
Fluoroquinolones are mainly used in ophthalmic antibiotic prophylaxis because of their broad spectrum activity and good ocular diffusion. But a single oral dose of fluoroquinolones can result in a serious source of tendinopathy and tendon rupture, especially in patients 60 years and older. It seems very important to investigate tendon toxicity of fluoroquinolones to improve the risk-benefit ratio in ophthalmologic antibiotic prophylaxis.


METHODS
and methods: The intrinsic tenotoxic potential of four fluoroquinolones (pefloxacin, ofloxacin, ciprofloxacin, levofloxacin) was directly evaluated on living adherent tendon cells in microplates. Cell viability and reactive oxygen species production was evaluated using neutral red, alamar blue, and dichlorofluorescin diacetate tests.


RESULTS
Results showed a loss of viability associated with free radical production depending on fluoroquinolone molecules. Pefloxacin appeared more tenotoxic but no study has confirmed its efficacy in surgical antibiotic prophylaxis and its use in the patient who is 60 years and older could be disputed. Ciprofloxacin is highly toxic with a low ocular diffusion and seems to be inappropriate for antibiotic prophylaxis. Ofloxacin and levofloxacin are less cytotoxic, associated with good ocular diffusion and a broad antibacterial spectrum.


CONCLUSIONS
Ofloxacin and levofloxacin seem to be good alternatives for improving the risk-benefit ratio in surgical antibiotic prophylaxis in patients 60 years and older.
Pouzaud
F
F
Unité de Pharmaco-Toxicologie Cellulaire, CHNO des XV-XX, 28, rue de Charenton, 75571 Paris cedex 12, France.
Rat
P
P
Cambourieu
C
C
Nourry
H
H
Warnet
J M
JM
D000125:Achilles Tendon / Q000293:injuries; D000368:Aged; D000818:Animals; D019072:Antibiotic Prophylaxis; D002478:Cells, Cultured / Q000187:drug effects; D002939:Ciprofloxacin / Q000633:toxicity; D003627:Data Interpretation, Statistical; D024841:Fluoroquinolones / Q000633:toxicity*; D005609:Free Radicals; D006801:Humans; D064704:Levofloxacin; D008875:Middle Aged; D015242:Ofloxacin / Q000633:toxicity; D013508:Ophthalmologic Surgical Procedures; D015366:Pefloxacin / Q000633:toxicity; D011817:Rabbits; D012421:Rupture; D013710:Tendons / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors
D024841:Fluoroquinolones; D005609:Free Radicals; D015366:Pefloxacin; D002939:Ciprofloxacin; D064704:Levofloxacin; D015242:Ofloxacin