title: Very high-field EPR study of glycyl radical enzymes.
abstract: This work shows that very high-field EPR spectroscopy allows a rather accurate determination of the g-tensor of protein radicals, including C-centered ones, and thus may be used as a probe for distinguishing a tyrosyl-, a glycyl-, or a tryptophanyl-radical. In this paper, we report the first complete analysis of the g-tensor of glycyl radical enzymes (anaerobic ribonucleotide reductase, pyruvate formate lyase, and benzylsuccinate synthase), thus providing new information on their EPR properties. Because the g-anisotropy is small, the complete resolution of the g-tensor could be only obtained at very high field (18.8 T).
authors.affiliation: High Magnetic Field Laboratory of Grenoble, CNRS-MPI, 25 av. des Martyrs, 38042 Grenoble, France. toia@labs.polycnrs-gre.fr
authors.forename: Carole
authors.initials: C
authors.lastname: Duboc-Toia
authors.forename: Alia K
authors.initials: AK
authors.lastname: Hassan
authors.forename: Etienne
authors.initials: E
authors.lastname: Mulliez
authors.forename: Sandrine
authors.initials: S
authors.lastname: Ollagnier-de Choudens
authors.forename: Marc
authors.initials: M
authors.lastname: Fontecave
authors.forename: Christina
authors.initials: C
authors.lastname: Leutwein
authors.forename: Johann
authors.initials: J
authors.lastname: Heider
mesh_terms: D016880:Anisotropy; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005609:Free Radicals / Q000737:chemistry; D005998:Glycine / Q000737:chemistry*; D012264:Ribonucleotide Reductases / Q000737:chemistry*
chemical_list: D005609:Free Radicals; D012264:Ribonucleotide Reductases; D005998:Glycine