[Substitution of BMEC-CM for hematopoietic stimulators in the culture of CFU-GM and HPP-CFC].
The effects of > 10 kD component of serum-free conditioned medium from murine bone marrow-derived endothelial cell line on the formation of CFU-GM and HPP-CFC were investigated. We found that > 10 kD component alone could stimulate CFU-GM colony formation in dose-dependent. Above- 10 kD component supplemented with GM-CSF not only resulted in significant increase of CFU-GM as compared with > 10 kD or GM-CSF alone, but also could stimulate HPP-CFC formation. The effect of > 10 kD component on the formation of HPP-CFC was also in dose-dependent. The component above- 10 kD combined with IL-3, IL-6, EPO, SCF or GM-CSF further enhanced HPP-CFC colony formation. The results suggest that > 10 kD component may be used as the substitutes of hematopoietic growth factors to stimulate the formation of CFU-GM and HPP-CFC.
Cheng
L M
LM
Research Laboratory of Blood Physiology, Hunan Medical University, Changsha 410078.
Wang
Q R
QR
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002460:Cell Line; D003114:Colony-Forming Units Assay; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D004727:Endothelium / Q000166:cytology*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008297:Male; D051379:Mice
D017077:Culture Media, Conditioned; D016178:Granulocyte-Macrophage Colony-Stimulating Factor