[Telomerase gene antisense oligodeoxynucleotide down-regulates telomerase activity in HL-60 cells].
OBJECTIVE
Human telomerase reverse transcriptase (hTERT) is a limiting component for telomerase activity. Telomerase inhibitor targeting telomerase RNA could arrest growth of tumor cells. This study was designed to explore the effect of hTERT gene antisense phosphorothioate oligodeoxynucleotide (ASODN) on telomerase activity in HL-60 cells.


METHODS
Telomeric repeat amplification protocol was used to determine telomerase activity. The expression levels of hTERT mRNA and protein were assayed by reverse transcriptase polymerase chain reaction (RT-PCR) and immunofluorescence using fluorescence isothiocyanate label respectively.


RESULTS
RT-PCR and immunofluorescence assay showed that the expression levels of hTERT mRNA and protein from HL-60 cells decreased with time after hTERT ASODN treatment. There was no difference in hTERT mRNA and protein levels between control and SODN-treated cells. Telomerase activity decreased when HL-60 cells were treated with ASODN for 48 h. Telomerase activity of HL-60 cells was significantly inhibited when treated with ASODN for 72h. There was no difference in telomerase activity levels between control and hTERT SODN-treated cells.


CONCLUSIONS
Telomerase activity could be down-regulated by hTERT ASODN in HL-60 cells.
He
Dong-mei
DM
Institute of Hematology, Jinan University Medical College, Guangzhou 510632, P. R. China.
Zhang
Huan
H
D002455:Cell Division / Q000187:drug effects; D004268:DNA-Binding Proteins; D015536:Down-Regulation; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019098:Telomerase / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D019098:Telomerase