title: Platelet endothelial cell adhesion molecule-1 in neutrophil emigration during acute bacterial pneumonia in mice and rats.
abstract: Platelet endothelial cell adhesion molecule-1 (PECAM-1) (CD31) is an adhesion molecule believed to mediate transendothelial migration of neutrophils and other leukocytes after CD11/CD18-mediated adhesion. Our study evaluated the role of PECAM-1 in neutrophil emigration across the pulmonary capillaries and the bronchial microvasculature using blocking anti-PECAM-1 antibodies in mice and rats. Neutrophil emigration was induced by Escherichia coli, a stimulus eliciting CD11/CD18-dependent emigration, or Streptococcus pneumoniae, a stimulus inducing CD11/CD18-independent emigration. Although anti-PECAM-1 antibodies partially inhibited glycogen-induced neutrophil emigration into the peritoneum, neutrophil emigration across either the pulmonary capillaries or the bronchial microvasculature in response to either E. coli or S. pneumoniae was not prevented when the function of PECAM-1 was inhibited in either mice or rats. There was also no increase in the number of intravascular neutrophils within the bronchial vessels after treatment with anti-PECAM-1 antibody. These studies indicate that either CD11/CD18-dependent or -independent adhesion pathways may lead to PECAM-1-independent transendothelial migration through the pulmonary or the bronchial endothelium.
authors.affiliation: Department of Pediatrics, Rainbow Babies and Children's Hospital, Case Western Reserve University, Cleveland, Ohio 44106, USA.
authors.forename: Sadatomo
authors.initials: S
authors.lastname: Tasaka
authors.forename: Lan
authors.initials: L
authors.lastname: Qin
authors.forename: Ariko
authors.initials: A
authors.lastname: Saijo
authors.forename: Steven M
authors.initials: SM
authors.lastname: Albelda
authors.forename: Horace M
authors.initials: HM
authors.lastname: DeLisser
authors.forename: Claire M
authors.initials: CM
authors.lastname: Doerschuk
mesh_terms: D000208:Acute Disease; D000704:Analysis of Variance; D000818:Animals; D018845:CD11 Antigens / Q000378:metabolism; D018821:CD18 Antigens / Q000032:analysis / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D004195:Disease Models, Animal; D004926:Escherichia coli; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis / Q000378:metabolism*; D018410:Pneumonia, Bacterial / Q000276:immunology* / Q000382:microbiology / Q000503:physiopathology*; D011897:Random Allocation; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D012680:Sensitivity and Specificity; D013296:Streptococcus pneumoniae
chemical_list: D018845:CD11 Antigens; D018821:CD18 Antigens; D019408:Platelet Endothelial Cell Adhesion Molecule-1