title: Human milk contains S100B protein.
abstract: The present study constitutes the first finding of the calcium-binding protein S100B and of its mRNA in human milk, as revealed by a quantitative immunoluminometric assay, by Western blot analysis and by reverse transcription-polymerase chain reaction (RT-PCR) assay followed by restriction enzyme digestion. The concentration of S100B in milk is markedly higher than that observed in other biological fluids such as cord blood, peripheral blood, urine, cerebrospinal fluid and amniotic fluid. This finding could be related to a possible trophic role, which has been hypothesized for the protein.
authors.affiliation: Department of Pediatrics, Giannina Gaslini Children's University Hospital, I-16147 Genoa, Italy.
authors.forename: Diego
authors.initials: D
authors.lastname: Gazzolo
authors.forename: Giovanni
authors.initials: G
authors.lastname: Monego
authors.forename: Valentina
authors.initials: V
authors.lastname: Corvino
authors.forename: Matteo
authors.initials: M
authors.lastname: Bruschettini
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Bruschettini
authors.forename: Giovanni
authors.initials: G
authors.lastname: Zelano
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Michetti
mesh_terms: D015153:Blotting, Western; D005260:Female; D006801:Humans; D008163:Luminescent Measurements; D008895:Milk, Human / Q000737:chemistry*; D009414:Nerve Growth Factors / Q000032:analysis* / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000032:analysis* / Q000737:chemistry
chemical_list: D009414:Nerve Growth Factors; D012333:RNA, Messenger; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579160:S100B protein, human