Immunohistochemistry of voltage-gated calcium channel alpha1B subunit in mouse cerebellum.
Secretion of neurotransmitters is initiated by voltage-gated calcium influx through presynaptic, voltage- gated N-type calcium channels. However, little is known about their cellular distribution in the mouse cerebellum. In the cerebellum, alpha1B immunoreactivity is found mainly on the cell bodies of all Purkinje cells. In addition, the immunoreactivity was detected on a subset of Purkinje cell dendrites, clustered to form a parasagittal array of bands. In the anterior lobe vermis, immunoreactive Purkinje cell dendrites form narrow stripes separated by broad bands of unstained dendrites. Moving caudally through the vermis, these stripes become thicker as a larger fraction of the Purkinje cell dendrites become immunoreactive. This localization study of the alpha1B pore-forming subunits in mouse cerebellum may guide future investigations of the role of calcium channels in neurological pathways.
Chung
Seung-Hyuk
SH
Department of Anatomy, College of Medicine, Konyang University, Nonsan, Chungnam, 320-711, South Korea.
Lee
Kyung-Youl
KY
Kim
Ki-Hyung
KH
Kim
Chul-Tae
CT
Lee
Nam-Seob
NS
Sawada
Kazuhiko
K
Haga
Hiromi
H
Lee
Byung-Chul
BC
Fukui
Yoshihiro
Y
Rhee
Man-Hee
MH
Jeong
Young-Gil
YG
D000818:Animals; D020864:Calcium Channels, N-Type / Q000378:metabolism*; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D011689:Purkinje Cells / Q000378:metabolism
D020864:Calcium Channels, N-Type