Tumouricidal activity of gilthead seabream (Sparus aurata L.) natural cytotoxic cells: the role played in vitro and in vivo by retinol acetate.
The natural cytotoxic activity of gilthead seabream head-kidney leucocytes was evaluated after in vitro incubation with retinol acetate as vitamin A source, and in samples taken from specimens receiving an intraperitoneal injection or a diet supplemented with this vitamin. Isolated leucocytes were incubated with 0 to 10(-10)m all-trans-retinol acetate-supplemented culture medium for 0, 6 or 24h and assayed for their tumouricidal activity which was found to increase for all the assayed concentrations and incubation times. Seabream specimens were intraperitoneally injected with 0 (control), 1.75 or 5.25 micro g retinol acetate 100 g(-1) biomass and sampled 1, 3 or 5 days post-injection. Leucocyte natural cytotoxic activity increased in a dose-dependent manner 1 and 3 days post-injection. When fish were fed a commercial diet supplemented with 0 (control), 50, 150 or 300 mg retinol acetate kg(-1) diet for 1, 2, 4 or 6 weeks, only fish which had been fed the highest supplement for 2 weeks showed any increase in head-kidney leucocyte cytotoxic activity. Serum was isolated and analysed for all-trans-retinol concentration by reverse-phase high-pressure-liquid-chromatography. The normal level was about 0.4 micro g ml(-1) serum, while treatment for 1 to 4 weeks with vitamin A increased this level. In conclusion, retinol acetate increases gilthead seabream head-kidney leucocyte cytotoxic activity both in vitro and in vivo.
Cuesta
A
A
Department of Cell Biology, Faculty of Biology, University of Murcia, 30100, Murcia, Spain.
Esteban
M A
MA
Meseguer
J
J
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D003601:Cytotoxicity Tests, Immunologic / Q000662:veterinary; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004224:Diterpenes; D004305:Dose-Response Relationship, Drug; D004306:Dose-Response Relationship, Immunologic; D007274:Injections, Intraperitoneal / Q000662:veterinary; D007668:Kidney / Q000166:cytology; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology*; D000084562:Retinyl Esters; D021541:Sea Bream / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014801:Vitamin A / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D004224:Diterpenes; D000084562:Retinyl Esters; D014801:Vitamin A; C009166:retinol acetate