[Several monomes from Tripterygium wilfordii inhibit proliferation of glioma cells in vitro].
OBJECTIVE
Researches indicated that Tripterygium wilfordii possess antitumor activity. The current study was designed to investigate inhibitive effect of several monomes of Tripterygium wilfordii on the proliferation of glioma cells.


METHODS
The effect of three monomes from Tripterygium wilfordii on the proliferation of glioma cell lines SHG44, C6, and U251 in vitro was examined by using MTT assay. Immunohistochemistry was used to examine the expression of Bax, Bcl-2 after treatment of triptolide and celastrol.


RESULTS
The proliferation of glioma cells was remarkably inhibited by triptolide and celastrol. They both increased expression of Bax and decreased expression of Bcl-2 in the SHG44 cells.


CONCLUSIONS
Triptolide and celastrol inhibit the proliferation of glioma cells in vitro, which was associated with promoting the expression of Bax and inhibiting the expression of Bcl-2 and accelerating cell apotosis.
Zhou
You-xin
YX
Department of Neurosurgery, First Affiliated Hospital of Suzhou University, Suzhou, 215006, P. R. China. yxzhou@public.sz.js.cn
Huang
Yu-lun
YL
Xu
Qi-nian
QN
Ye
Ming
M
Sun
Cheng-fa
CF
Zhou
Dai
D
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology; D004852:Epoxy Compounds; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D053978:Pentacyclic Triterpenes; D010616:Phenanthrenes; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D029757:Tripterygium; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D004224:Diterpenes; D004852:Epoxy Compounds; D053978:Pentacyclic Triterpenes; D010616:Phenanthrenes; D010936:Plant Extracts; D014315:Triterpenes; C471118:wilforol A; C001899:triptolide; C050414:celastrol