[Restorative morphogenesis in vitro of the annual Medicago species in presence of benzylaminopurine].
Four annual medick species (Medicago orbicularis (L.) All., M. rigidula (L.) Desr., M. scutellata (L.) Miller, and M. rugosa Desr.) were used as model objects for studying the spectrum of morphogenetic reactions in vitro. The seeds were incubated on nutrient media with benzylaminopurine at different concentrations until germination and, thereafter, the explants of seedlings were cultivated in order to obtain primary calluses and morphogenetic structures. Normal and abnormal (with reduced root and/or apex) seedlings were cultivated in the presence of benzylaminopurine. Further cultivation of explants from the seedlings of both types showed a considerable intra- and interspecific polymorphism by the capacity for callusogenesis, frequency of primary restorative reactions, and pattern of microreproduction in vitro. In the control (the seeds were incubated on a hormone-free medium), no cases of microreproduction by way of organogenesis or somatic embryogenesis were observed. In all experimental variants, the restorative reactions preceded microreproduction in vitro.
Smolenskaia
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Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, pr. Akademika Lavrent'eva 10, Novosibirsk, 630090 Russia.
Ibragimova
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D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D001593:Benzyl Compounds; D003470:Culture Media; D018525:Germination; D018527:Hydroponics / Q000379:methods; D007701:Kinetin; D029902:Medicago / Q000187:drug effects* / Q000254:growth & development*; D009024:Morphogenesis; D010937:Plant Growth Regulators / Q000494:pharmacology*; D011687:Purines; D012639:Seeds / Q000187:drug effects / Q000254:growth & development; D013045:Species Specificity
D001593:Benzyl Compounds; D003470:Culture Media; D010937:Plant Growth Regulators; D011687:Purines; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin