[Role of phorbol 12-myristate 13-acetate on cytokine expression in JAR trophoblast cell line].
OBJECTIVE
To investigate whether phorbol 12-myristate 13-acetate (PMA) modifies the invasive ability of trophoblast cells by regulating their cytokine productions.


METHODS
Reverse transcriptase-polymerase chain reaction was used to examine the effect of PMA on the expression of cytokines which regulated the invasive ability of trophoblast cells.


RESULTS
Prior to PMA treatment, expressions of the cytokins including hepatocyte growth factor (HGF), interleukin (IL)-1beta, insulin-like growth factor (IGF)-II, transforming growth factor (TGF)- beta and vascular endothelial growth factor (VEGF) were all detected in JAR cells, only with the exception of IGF-I. After incubation with 100 nmol/L PMA for 24 h, the cells showed strong expression of IL-1beta, HGF and IGF-II, with reduced expression of TGF-beta2 and TGF-beta3.


CONCLUSIONS
By regulating the autocrine of these cytokines, PMA exercises its effect to enhance the invasive ability of trophoblast or choriocarcinoma cells.
Zhang
Xi-qian
XQ
Department of Obstetrics and Gynecology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. cherd075@yahoo.com.cn
Xing
Fu-qi
FQ
Li
Hong
H
Chen
Si-mei
SM
Pang
Zhan-jun
ZJ
Huang
Min-zhen
MZ
D002460:Cell Line; D002822:Choriocarcinoma / Q000473:pathology*; D016207:Cytokines / Q000235:genetics*; D016228:Endothelial Growth Factors / Q000235:genetics; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D008222:Lymphokines / Q000235:genetics; D009361:Neoplasm Invasiveness; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D013002:Somatomedins / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000235:genetics; D014327:Trophoblasts / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D014594:Uterine Neoplasms / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016207:Cytokines; D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D012333:RNA, Messenger; D013002:Somatomedins; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D013755:Tetradecanoylphorbol Acetate