Endothelin-1 receptor subtypes expression and binding in a perfused rat model of myocardial infarction.
Endothelin-1 (ET-1) pathophysiologic actions are mediated via binding with two receptor subtypes, ET(A) and ET(B). Release of ET-1 from endocardial endothelial cells and cardiac myocytes can modulate heart tissue necrosis and alterations. This study investigates the remodeling processes in Sprague-Dawley rats of myocardial infarction (MI) induced by ligating the left anterior descending coronary artery. Histological studies were done on cell type distribution using cell specific markers and Western blot analysis to localize ET-1 receptor subtypes and assess their expression post-MI. In addition, the binding kinetics of ET-1 with its receptors in heart perfusion, inlet via the aortic lumen and effluent outlet via the right atrium, between two animal model-subgroups were done: (1) sham-operated, and sham-operated-CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate)-treated; and (2) MI-operated, and MI-operated-CHAPS-treated. Effluent ET-1 concentration was plotted vs. time using a physical model for 1:1 ligand-receptor binding at coronary endothelium and myocytes. First order impulse function was used to calculate the affinity constants. In MI hearts, fluorescence activity increased for ET(A) vs. ET(B) across areas of the muscle compared to normal hearts. Western blotting showed upregulation of ET(A) and ET(B) receptors in MI compared with normal hearts. Results of ET-1 binding affinity post-MI indicated drastic reduction in spite the upregulation of ET(B) on coronary endothelium. Furthermore, substantial affinity increase was observed between ET-1 binding with ET(A) at the myocyte site. These findings stipulate that during 1 month post-MI some biochemical and hormonal effects could alter ET-1 receptor subtype(s) regulation and pharmacodynamics thus predisposing to cardiac hypertrophy and mitogenesis.
Bikhazi
Anwar B
AB
Department of Physiology, Faculty of Medicine, American University of Beirut, Beirut, Lebanon. ab04@aub.edu.lb
Khalifeh
Adeeb M
AM
Jaroudi
Wael A
WA
Saadeddine
Rihab E
RE
Jurjus
Abdu R
AR
El-Sabban
Marwan E
ME
Bitar
Khalil M
KM
D000818:Animals; D015153:Blotting, Western; D002793:Cholic Acids / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D004195:Disease Models, Animal; D019084:Fluorescent Antibody Technique, Indirect; D006321:Heart / Q000187:drug effects; D008297:Male; D009203:Myocardial Infarction / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D044022:Receptor, Endothelin A; D017466:Receptors, Endothelin / Q000145:classification / Q000378:metabolism*; D020257:Ventricular Remodeling / Q000502:physiology*
D002793:Cholic Acids; D003902:Detergents; D044022:Receptor, Endothelin A; D017466:Receptors, Endothelin; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate