title: Hydrogen exchange in a large 29 kD protein and characterization of molten globule aggregation by NMR.
abstract: The nature of denatured ensembles of the enzyme human carbonic anhydrase (HCA) has been extensively studied by various methods in the past. The protein constitutes an interesting model for folding studies that does not unfold by a simple two-state transition, instead a molten globule intermediate is highly populated at 1.5 M GuHCl. In this work, NMR and H/D exchange studies have been conducted on one of the isozymes, HCA I. The H/D exchange studies, which were enabled by the previously obtained resonance assignment of HCA I, have been used to identify unfolded forms that are accessible from the native state. In addition, the GuHCl-induced unfolded states of HCA I have also been characterized by NMR at GuHCl concentrations in the 0-5 M range. The most important findings in this work are as follows: (1) Amide protons located in the center of the beta-sheet require global unfolding events for efficient H/D exchange. (2) The molten globule and the native state give similar protection against H/D exchange for all of the observable amide protons (i.e., water seems not to efficiently penetrate the interior of the molten globule). (3) At high protein concentrations, the molten globule can form large aggregates, which are not detectable by solution-state NMR methods. (4) The unfolded state (U), present at GuHCl concentrations above 2 M, is composed of an ensemble of conformations having residual structures with different stabilities.
authors.affiliation: Department of Biochemistry and Department of Organic Chemistry, Umeå University, SE-901 87 Umeå, Sweden.
authors.forename: Annika
authors.initials: A
authors.lastname: Kjellsson
authors.forename: Ingmar
authors.initials: I
authors.lastname: Sethson
authors.forename: Bengt-Harald
authors.initials: BH
authors.lastname: Jonsson
mesh_terms: D000577:Amides / Q000145:classification; D024401:Carbonic Anhydrase I / Q000737:chemistry*; D003903:Deuterium / Q000737:chemistry; D019791:Guanidine / Q000737:chemistry; D006801:Humans; D008956:Models, Chemical; D008970:Molecular Weight; D009587:Nitrogen Isotopes / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011522:Protons / Q000145:classification; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013816:Thermodynamics
chemical_list: D000577:Amides; D009587:Nitrogen Isotopes; D011522:Protons; D003903:Deuterium; D024401:Carbonic Anhydrase I; D019791:Guanidine