title: CO2 laser surgery for vulvar intraepithelial neoplasia. Excisional, destructive and combined techniques.
abstract: OBJECTIVE
  To evaluate CO2 laser excision, vaporization and combined techniques for treatment of vulvar intraepithelial neoplasia (VIN).
  
  
  METHODS
  Thirty-nine cases of VIN 3, 15 cases of VIN 2 and 9 of VIN 1, for a total of 63 patients with histologically proven VIN, underwent laser excision or vaporization under colposcopic guidance, using local anesthesia, in an outpatient setting or after day-surgery admission. Clinical aspects, cervical intraepithelial neoplasia (CIN) and vaginal intraepithelial neoplasia (VaIN) association, types of CO2 laser treatment, follow-up, recurrences and second treatments were evaluated.
  
  
  RESULTS
  Twenty-seven (41.3%) patients underwent laser vaporization, and 37 (58.7%) with VIN 3, underwent laser excision or the combined technique. Colposcopic and biopsy examinations of patients with VIN revealed three cases of CIN 3 and nine cases of VaIN 3; two patients had concomitant VIN 3, CIN 3 and VaIN 3. Local anesthesia, using 2% carbocaine, and outpatient or day-surgery treatments were possible in all cases. A small incidence of intraoperative complications (4.8%) and absence of postoperative complications were observed. A single session was curative in 76.9% of patients treated with laser vaporization and in 78.4% of those treated with laser excision. Eleven cases of recurrent VIN and two cases of invasive vulvar carcinoma were observed during follow-up. A second laser procedure was carred out in all cases of relapsed VIN, with an overall cure rate of 96.8% after two treatments. Radical vulvectomy associated with inguinal-femoral lymphadenectomy was performed in the two cases of invasive carcinoma.
  
  
  CONCLUSIONS
  CO2 laser surgery permits treatment of VIN in an outpatient or day-surgery setting under local anesthesia with excellent cosmetic and functional results. The treatment can also be adjusted to the patient's specific needs, with the possibility of calibrating the depth of the vaporized and removed tissues. Excisional treatment is the preferred method because it permits histologic evaluation of the excised tissue and detection of possible occult early invasion.
authors.affiliation: Department of Gynecology, Perinatology and Human Reproduction, University of Florence, Florence, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Penna
authors.forename: M Grazia
authors.initials: MG
authors.lastname: Fallani
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Fambrini
authors.forename: Elisa
authors.initials: E
authors.lastname: Zipoli
authors.forename: Mauro
authors.initials: M
authors.lastname: Marchionni
mesh_terms: D000328:Adult; D000368:Aged; D000553:Ambulatory Care; D000772:Anesthesia, Local; D002245:Carbon Dioxide; D002278:Carcinoma in Situ / Q000453:epidemiology / Q000473:pathology / Q000601:surgery*; D003127:Colposcopy; D005260:Female; D006801:Humans; D007558:Italy / Q000453:epidemiology; D053685:Laser Therapy / Q000379:methods / Q000592:standards*; D008499:Medical Records; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000453:epidemiology / Q000473:pathology / Q000601:surgery*; D011183:Postoperative Complications; D012189:Retrospective Studies; D014846:Vulvar Neoplasms / Q000453:epidemiology / Q000473:pathology / Q000601:surgery*
chemical_list: D002245:Carbon Dioxide