Characterizing stress gene expression in reef-building corals exposed to the mosquitoside dibrom.
We characterize two genes expressed in Acropora cervicornis upon exposure to 0.5 microg/l of dibrom, a pesticide used for mosquito control in the Florida Keys. Fragments of these genes were isolated, sequenced, and developed into chemiluminescent probes for Northern slot blots. Expression of target transcripts was detected in corals exposed to a variety of stressors including organophosphates, organochlorines, heavy metals, naphthalene, and temperature. Within the context of stressors examined, the D25 probe demonstrates toxicant and concentration specificity for organophosphates, whereas the D50 probe had broader specificity, detecting transcripts in corals exposed to dibrom, naphthalene, and temperature stress. After characterizing specificity in the lab, these probes were used on field samples taken from the Florida Keys. Both probes detected their targets in samples taken from the upper Florida Keys in August 2000. Preliminary search of sequence databases suggest similarity exists between D25 and a thioesterase.
Morgan
Michael B
MB
Georgia Institute of Technology, School of Biology, Atlanta, GA 30332-0230, USA. biommm@langate.gsu.edu
Snell
Terry W
TW
D000595:Amino Acid Sequence; D000818:Animals; D037421:Anthozoa / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects; D007306:Insecticides / Q000633:toxicity*; D008969:Molecular Sequence Data; D009267:Naled / Q000633:toxicity*; D016133:Polymerase Chain Reaction; D012623:Seawater
D018076:DNA, Complementary; D007306:Insecticides; D009267:Naled