Evaluation of a Apo-1/Fas promoter polymorphism in Korean stroke patients.
Apoptosis has been implicated in the pathogenesis of neurodegenerative diseases such as stroke and Alzheimer's disease. Apo-1/Fas gene is one of the mediators of apoptosis in stroke. Mval polymorphism is the first polymorphic marker identified in the Apo-1/Fas gene promoter, which was typed by PCR and followed by Mval digestion and gel electrophoresis. DNA isolated from peripheral blood collected from 91 stroke patients and 103 healthy blood donors was used for genotypes of GG, GA and AA by sequence specific primer PCR. Mval polymorphism was examined based on Fas gene promotor region by restriction fragment length polymorphism (RFLP). The Fas-GG genotype was the least frequent in patients with stroke and healthy controls (P = 0.57). In normal Korean controls the Mval polymorphism GA, AA and GG were 48.6%, 34.9% and 16.5%. In stroke patients were 56.2%, 29.6% and 14.2% respectively. And the allelic frequencies of Mval*2 (G) allele were less frequent than Mval*1 (A) allele in patients with stroke and healthy controls (P = 0.76). In normal Korean controls Mval*1 (A) and Mval*2 (G) alleles were 59.2% and 40.8%. In stroke patients were 57.6% and 42.4%, respectively. Our results, pending confirmation in a larger study, indicate that the Fas genotype may not appear to be a risk factor for stroke in Korean stroke patients.
Seo
Jung-Chul
JC
Department of Acupuncture and Moxibustion, College of Oriental Medicine, Kyungsan University, Gumi 730-090, Korea.
Han
Sang-Won
SW
Yin
Chang-Sik
CS
Koh
Hyung-Kyun
HK
Kim
Chang-Hwan
CH
Kim
Ee-Hwa
EH
Leem
Kang-Hyun
KH
Lee
Hyang-Sook
HS
Park
Hi-Joon
HJ
Kim
Soon-Ae
SA
Choe
Bong-Keun
BK
Lee
Hee-Jae
HJ
Yim
Sung-Vin
SV
Kim
Chang-Ju
CJ
Chung
Joo-Ho
JH
D000328:Adult; D000368:Aged; D002544:Cerebral Infarction / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007723:Korea; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D019014:fas Receptor / Q000235:genetics*
D019014:fas Receptor