Analysis of loss of chromosome 10q, DMBT1 homozygous deletions, and PTEN mutations in oligodendrogliomas.
OBJECTIVE
Chromosomal deletions of 10q and candidate genes such as PTEN and DMBT1 have been thoroughly investigated in glioblastomas but few data specifically address oligodendrogliomas.


METHODS
In this study, 39 pure oligodendrogliomas were investigated for loss of heterozygosity (LOH) on 10q, PTEN mutations, and DMBT1 homozygous deletions. The LOH on 10q was found in 19 (48%) of 39 oligodendrogliomas and was closely related to anaplasia (p = 0.02), shorter time to progression (p = 0.0005), and poorer survival (p = 0.035). The DMBT1 homozygous deletions were found in 10 (26%) of 39 oligodendrogliomas but only one PTEN mutation was detected. The LOH on 10q is a strong predictor of survival and could be a valuable prognostic marker in oligodendrogliomas.


CONCLUSIONS
Frequent inactivation of DMBT1 contrasting with rare mutations of PTEN may indicate that DMBT1 is preferentially involved in oligodendrogliomas. Nevertheless, the absence of a correlation with survival makes the role of DMBT1 in tumorigenesis still questionable and warrants further investigation.
Sanson
Marc
M
INSERM U495, Laboratoire de Biologie des Interactions Neurones-Glie, Groupe Hospitalier Pitié-Salpêtrière and Université Pierre et Marie Curie, Paris, France. m.sanson@psl.ap-hop-paris.fr
Leuraud
Pascal
P
Aguirre-Cruz
Lucinda
L
He
Jié
J
Marie
Yannick
Y
Cartalat-Carel
Stéphanie
S
Mokhtari
Karima
K
Duffau
Hugues
H
Delattre
Jean-Yves
JY
Hoang-Xuan
Khê
K
D000373:Agglutinins; D001932:Brain Neoplasms / Q000235:genetics* / Q000401:mortality; D002135:Calcium-Binding Proteins; D002879:Chromosomes, Human, Pair 10; D004268:DNA-Binding Proteins; D017353:Gene Deletion; D005819:Genetic Markers; D006720:Homozygote; D006801:Humans; D019656:Loss of Heterozygosity; D009837:Oligodendroglioma / Q000235:genetics* / Q000401:mortality; D051059:PTEN Phosphohydrolase; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D011237:Predictive Value of Tests; D011379:Prognosis; D011956:Receptors, Cell Surface / Q000235:genetics*; D016019:Survival Analysis; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D000373:Agglutinins; D002135:Calcium-Binding Proteins; C108174:DMBT1 protein, human; D004268:DNA-Binding Proteins; D005819:Genetic Markers; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human