[Establishment of models for purging leukemic cells from the grafts in vitro].
Using a fluorochrome Calcein-AM, leukemia cells were labeled and seeded into cell lines or bone marrow cells to establish three cell-models of grafts with leukemia. These cell-models were engaged with CD34 immunomagnetic beads and the purging efficacy was evaluated using both fluorescence microscopy and flow cytometry. The results showed that the cell-models established in this study could be evaluated successfully not only with fluorescence microscopy but also flow cytometry. After CD34 positive selection, KG1a cells were removed by (0.98 +/- 0.09) log in model II and NALM-6 cells were removed by (1.82 +/- 0.51) log in model III, respectively. It is concluded that the models established in this study are stable and direct with an excellent reproducibility and an accuracy, which can be used to evaluate purging efficacy of leukemia cells in model graft using immunomagnetic selection and the experimental studies on tumorcidal effect in vitro.
Sun
Fei
F
The Jiaxing First Hospital of Zhejiang Province, Jiaxing, 314000, China. Y-M-Tang@yahoo.com
Tang
Yong-Min
YM
Shen
Hong-Qiang
HQ
Qian
Bai-Qin
BQ
Song
Hua
H
Yang
Shi-Long
SL
Shi
Shu-Wen
SW
Xu
Wei-Qun
WQ
D016465:Bone Marrow Purging; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D007938:Leukemia / Q000473:pathology / Q000628:therapy*; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D014182:Transplantation, Autologous