title: The effect of pH on the foam fractionation of beta-glucosidase and cellulase.
abstract: The surface tension-pH profile of beta-glucosidase was established to determine its relationship to the corresponding profile of cellulase and to the foam fractionation of that cellulase. The goal of this work was to determine the optimal foaming points for both cellulase and cellobiase. This data may prove useful in the separation of certain components of cellulase, since the non-foaming hydrophilic beta-glucosidase does not foam as well as the hydrophobic components of cellulase at low concentrations. A key finding from these experiments was that there are two local minima in the surface tension-pH trajectory for Trichoderma reesei cellulase, as contrasted to the usual single minimum. The lower of these minimum points corresponds to the cellulase isoelectric point. The double minimum surface tension-pH profile was also observed for cellobiase alone. The optimal foaming pH for cellobiase alone was determined to be around 10.5, while for cellulase it was between 6 and 9.
authors.affiliation: Department of Chemical Engineering, Vanderbilt University, Nashville, TN 37235, USA.
authors.forename: William D
authors.initials: WD
authors.lastname: Lambert
authors.forename: Liping
authors.initials: L
authors.lastname: Du
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Ma
authors.forename: Veara
authors.initials: V
authors.lastname: Loha
authors.forename: Vorakan
authors.initials: V
authors.lastname: Burapatana
authors.forename: Ales
authors.initials: A
authors.lastname: Prokop
authors.forename: Robert D
authors.initials: RD
authors.lastname: Tanner
authors.forename: Neville B
authors.initials: NB
authors.lastname: Pamment
mesh_terms: D002480:Cellulase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D013500:Surface Tension; D014242:Trichoderma / Q000201:enzymology; D001617:beta-Glucosidase / Q000737:chemistry*
chemical_list: D001617:beta-Glucosidase; D002480:Cellulase