[A modified assay for phospholipids in blood serum].
An antiscorbutate reduction method for serum phospholipids assay was modified by decreasing the consumed quantity of sulphuric acid and perchloric acid to 30% of that before modification, and the quantity of serum to 40% of that before modification. The digesting time was only 50%-62% of that before modification. The coefficients of variation (CV) were 3.4% and 3.7% for two batches of assays. Recovery of phosphotidylcholine added to serum ranged form 94% to 109%, averagely 100.8%. Serum phospholipids assay of 50 normal adults by the methods before and after modification showed statistically no significant difference (2.813 +/- 0.333 mmol/L vs 2.828 +/- 0.316 mmol/L, P > 0.05), but good correlation (r = 0.991). The results indicate that the modified assay for serum phospholipids can decrease the use of serum and reagents, especially the harmful acids, and get the same result as that before modification.
Fang
D
D
Department of Biochemistry, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
Liu
B
B
D001205:Ascorbic Acid; D006801:Humans; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000097:blood*
D010743:Phospholipids; D001205:Ascorbic Acid