[Effect of Salvia miltiorrhiza on autocrining growth factor by fibroblasts cultured in vitro].
OBJECTIVE
To investigate the mechanism of overhealing alleviation by salvia miltiorrhiza (SM) in wound healing.


METHODS
Fibroblasts were cultured in vitro, and SM was applied with different concentrations (40, 80, 160 and 320 micrograms/ml) and time(the 1st, 2nd, 3rd, 4th and 5th days) to influence their autocrine. The levels of transforming growth factor-beta 1 (TGF-beta 1) and epidermal growth factor (EGF) were determined by ELIAS and radioimmunoassay respectively.


RESULTS
The SM could inhibit autocrine of TGF-beta 1 by fibroblasts (P < 0.05). However, it did not affect autocrine of EGF (P > 0.05).


CONCLUSIONS
The above results indicate that SM reduces overhealing by inhibiting the autocrine of TGF-beta 1 selectively.
Wang
Yi-ming
YM
Department of Pediatric Surgery, West China Hospital, Sichuan University, Chengdu Sichuan, P. R. China 610041.
Wei
Fu-kang
FK
Liu
Min
M
D002455:Cell Division; D002478:Cells, Cultured; D002648:Child; D017439:Cicatrix, Hypertrophic / Q000473:pathology; D003094:Collagen / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000187:drug effects*; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D027561:Salvia miltiorrhiza; D013997:Time Factors; D015291:Transforming Growth Factors / Q000187:drug effects*; D014945:Wound Healing
D004365:Drugs, Chinese Herbal; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D003094:Collagen