[Study on telomerase activity and expression of hTERT, c-myc and bcl-2 during terminal differentiation of HL-60 cells induced by retinoic acid].
The study was to explore the telomerase activity and the expression of hTERT, c-myc and bcl-2 mRNA during terminal differentiation of HL-60 cells induced by all trans-retinoic acid (ATRA) and to study the possible molecular mechanism. By use of the model of differentiated HL-60 cells induced by ATRA, the telomerase activity was determined by TRAP-PCR-ELISA and the expression of hTERT, c-myc, bcl-2 mRNA was detected by RT-PCR in differentiated HL-60 cells. The results showed that during differentiation of HL-60 cells, the telomerase activity was decreased, the expression of hTERT, c-myc and bcl-2 mRNA was downregulated, and the downregulation of hTERT occurred prior to suppression of telomerase activity. It is concluded that the telomerase activity is related to decrease expression of hTERT, c-myc and bcl-2 mRNA during HL-60 cell differentiation induced by ATRA.
Wu
Li-De
LD
Fujian Institute of Hematology, Union Hospital of Fujian Medical University, Fuzhou 350001, China.
Chen
Yuan-Zhong
YZ
Li
Nai-Nong
NN
Wu
Yong
Y
D002454:Cell Differentiation; D004268:DNA-Binding Proteins; D015972:Gene Expression Regulation, Neoplastic; D019254:Genes, bcl-2; D016259:Genes, myc; D018922:HL-60 Cells; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology*
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D014212:Tretinoin; D019098:Telomerase