[Variable tandem repeat VcA of Vibrio cholerae].
Computer analysis revealed seven potential variable-number tandem-repeat (VNTR) loci in the Vibrio cholerae genome. Specific primers were designed to amplify locus VcA located on chromosome 2 and containing a TGCTGT repeat. The locus was found in all tested strains from a V. cholerae strain collection, the repeat number varying 3 to 23. In total, 14 VcA alleles were observed. The VcA locus was proposed as a marker for the molecular typing of V. cholerae strains.
Vodop'ianov
S O
SO
Institute of Antiplague Research, Rostov-on-Don, 344007 Russia. vodopyanov_s@yahoo.com
Oleĭnikov
I P
IP
Goncharov
E K
EK
Duvanova
O V
OV
Mishan'kin
M B
MB
Suchkov
I Iu
IIu
Vodop'ianov
A S
AS
Shishiianu
M V
MV
Shcherbakova
E V
EV
Kornienko
I V
IV
Mishan'kin
B N
BN
D000465:Algorithms; D000483:Alleles; D015373:Bacterial Typing Techniques / Q000379:methods; D001483:Base Sequence; D017931:DNA Primers; D030561:Databases, Nucleic Acid; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics*
D017931:DNA Primers