Cytokinins: new apoptotic inducers in plants.
High concentrations of cytokinins block cell proliferation and induce programmed cell death (PCD) in both carrot ( Daucus carota L.) and Arabidopsis thaliana (L.) Heynh. cell cultures [13 and 27 micro M N(6)-benzylaminopurine (BAP), respectively]. In the present work, cell death was scored by Evan's blue staining and was also demonstrated to be programmed by various parameters, including chromatin condensation, oligonucleosomal DNA degradation (laddering), and release of cytochrome c from mitochondria. In carrot cells, this induction takes approximately 24 h, with proliferating cells being more sensitive than quiescent ones. Two hormones, namely abscisic acid and 2,4-dichlorophenoxyacetic acid (2,4-D), protect cells against the cytokinin-induced death. PCD is not merely a consequence of the inability of the culture to proliferate, since high levels of 2,4-D block carrot cell proliferation without promoting PCD. Increased ethylene production was also observed in BAP-treated cultures, although this increase was not responsible for PCD because inhibitors of ethylene synthesis and action did not block PCD in BAP-treated cultures. Programmed cell death in the form of DNA laddering was also seen in plants treated with cytokinins. This process was accompanied by accelerated senescence in the form of leaf yellowing.
Carimi
Francesco
F
Centro di Studio delle Biomembrane, CNR, Via G. Colombo 3, 35131 Padova, Italy. carimi@civ.bio.unipd.it
Zottini
Michela
M
Formentin
Elide
E
Terzi
Mario
M
Lo Schiavo
Fiorella
F
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D000040:Abscisic Acid / Q000494:pharmacology; D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D017360:Arabidopsis / Q000187:drug effects* / Q000502:physiology; D001593:Benzyl Compounds; D002478:Cells, Cultured; D002843:Chromatin / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D003583:Cytokinins / Q000494:pharmacology*; D003600:Cytosol / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D018552:Daucus carota / Q000187:drug effects* / Q000502:physiology; D007701:Kinetin; D008928:Mitochondria / Q000187:drug effects; D010937:Plant Growth Regulators / Q000494:pharmacology*; D011687:Purines
D001593:Benzyl Compounds; D002843:Chromatin; D003574:Cytochrome c Group; D003583:Cytokinins; D010937:Plant Growth Regulators; D011687:Purines; D015084:2,4-Dichlorophenoxyacetic Acid; D000040:Abscisic Acid; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin