[Antisense oligonucleotide of rat gp130 blocked the inhibitory effect of rhIL-6 on proliferation of the rat acute myeloid leukemia cell line R2 in vitro cultures].
The second nonligand binding chain of IL-6 receptor (IL-6R), the membrane glycoprotein with 130 kD mol wt (gp130), is responsible for the signal transduction of IL-6 biological activity. Our experiments indicated that the rat gp130 molecule, which was expressed in the rat acute myeloid leukemia cell line R2, could associate with the complex of rhIL-6 and membrane human IL-6R molecule and transduce the inhibition-inducing signal on R2 cells. In the present study, antisense oligonucleotides of rat gp130 were synthesized and uptaked into the R2 cells. Then the effects of the antisense or sense nucleic acids of rat gp130 on the inhibition induced by rhIL-6 in the R2 cell were investigated. Our results show that the antisense oligonucleotide of rat gp130 blocked the inhibitory effect of rhIL-6 on the R2 cells by (45 +/- 7)% at the optimal concentration
Ren
Yun-Fang
YF
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
Feng
Ming-Chiu
MC
D000208:Acute Disease; D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D002455:Cell Division / Q000187:drug effects; D050822:Cytokine Receptor gp130; D004347:Drug Interactions; D015850:Interleukin-6 / Q000494:pharmacology*; D007951:Leukemia, Myeloid / Q000473:pathology*; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D014407:Tumor Cells, Cultured; D000093242:Interleukin-6 Inhibitors
D015703:Antigens, CD; D050822:Cytokine Receptor gp130; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D016376:Oligonucleotides, Antisense; D000093242:Interleukin-6 Inhibitors; C494201:Il6st protein, rat