title: [Whether the impairment of grafted liver was induced by the inflammatory cells in cold and warm ischemia after transplantation].
abstract: OBJECTIVE
  To investigate whether the impairment of grafted liver after transplantation was induced by the same inflammatory cells in cold and warm ischemia.
  
  
  METHODS
  Male SD rats were divided into two groups randomly, 24 grafted livers in each group were stored for 120 or 240 min at 4 degrees Centigrade Ringer's solution. Also male SD rats were divided into three groups, in which 24 grafted livers in each group were experienced warm ischemia ranged from 90, 120 to 150 min from non-heart-beating donor. The recipients were killed after 1, 3, 6, and 24 hours of transplantation for sample collection.
  
  
  RESULTS
  Along with the prolongation of cold and warm ischemia time, the serum ALT and AST levels were increased gradually after transplantation. Light microscopy showed some necroses in hepatocytes after 3 and 6 hours of transplantation in cold ischemia, and some neutrophilic infiltration in sinusoids. There were a large number of hepatocytes necroses after 3, 6 hours of transplantation in warm ischemia from non-heart-beating donor and a lot of lymphocytic infiltration in sinusoids. The findings in electron microscopy were as the same as those found in light microscopy, and the lymphocytes which infiltrated in sinusoids in warm ischemia were identified as T lymphocytes in electron microscopy.
  
  
  CONCLUSIONS
  The impairment of grafted livers after transplantation seems to be induced by two different inflammatory cells in cold and warm ischemia, that is, neutrophils mediate the cold ischemia-reperfusion, and T lymphocytes mediate the warm ischemia-reperfusion from non-heart-beating donor.
authors.affiliation: Department of Surgery, Ruijin Hospital, Shanghai Secondary Medical University, Shanghai 200025, China.
authors.forename: Tanglei
authors.initials: T
authors.lastname: Shao
authors.forename: Weiyao
authors.initials: W
authors.lastname: Cai
authors.forename: Weiping
authors.initials: W
authors.lastname: Yang
authors.forename: Mingjun
authors.initials: M
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Zhongmin
authors.initials: Z
authors.lastname: Di
authors.forename: Guangwen
authors.initials: G
authors.lastname: Zhou
authors.forename: Hongwei
authors.initials: H
authors.lastname: Li
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D006085:Graft Survival / Q000502:physiology; D022781:Hepatocytes / Q000473:pathology / Q000648:ultrastructure; D008099:Liver / Q000098:blood supply / Q000503:physiopathology* / Q000648:ultrastructure; D016031:Liver Transplantation / Q000502:physiology*; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology; D013601:T-Lymphocytes / Q000502:physiology*; D013696:Temperature; D013997:Time Factors
chemical_list: D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase