[Antiviral activity of antisense oligonucleotides to human cytomegalovirus].
Human cytomegalovirus(HCMV) is one of the important pathogens to human being. At present there is no specific antiviral drugs. In order to find an effective antisense phosphorothioate deoxyoligonucleotide(ASON) against--HCMV, the antiviral activity was observed in this study. Three ASON complementary to UL36 gene were synthesized according to immediate early gene sequence of HCMV, and the antiviral activity was evaluated in vitro using human embryonic lung cells as targeted cells of virus infections. The result showed that ASON targeting to splice donor had fairly strong antiviral activity. The specific ASON has sure antiviral activity. As a new means, it may be used to prevent and treat human CMV infections.
Huang
W
W
Institute of Radiation Medicine, Chinese Academy of Military Medical Sciences, Beijing 100850.
Liu
Z
Z
Bu
F
F
D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000187:drug effects*; D005333:Fetus; D006801:Humans; D008168:Lung / Q000166:cytology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D009946:Organothiophosphorus Compounds / Q000494:pharmacology*; D013873:Thionucleotides
D000998:Antiviral Agents; C428115:Olg antisense phosphorothioate oligonucleotide; D016376:Oligonucleotides, Antisense; D009946:Organothiophosphorus Compounds; D013873:Thionucleotides