title: Association of the kinesin motor KIF1A with the multimodular protein liprin-alpha.
abstract: Liprin-alpha/SYD-2 is a multimodular scaffolding protein important for presynaptic differentiation and postsynaptic targeting of alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid glutamate receptors. However, the molecular mechanisms underlying these functions remain largely unknown. Here we report that liprin-alpha interacts with the neuron-specific kinesin motor KIF1A. KIF1A colocalizes with liprin-alpha in various subcellular regions of neurons. KIF1A coaccumulates with liprin-alpha in ligated sciatic nerves. KIF1A cofractionates and coimmunopreciptates with liprin-alpha and various liprin-alpha-associated membrane, signaling, and scaffolding proteins including alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid receptors, GRIP/ABP, RIM, GIT1, and beta PIX. These results suggest that liprin-alpha functions as a KIF1A receptor, linking KIF1A to various liprin-alpha-associated proteins for their transport in neurons.
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
authors.forename: Hyewon
authors.initials: H
authors.lastname: Shin
authors.forename: Michael
authors.initials: M
authors.lastname: Wyszynski
authors.forename: Kyung-Hye
authors.initials: KH
authors.lastname: Huh
authors.forename: Juli G
authors.initials: JG
authors.lastname: Valtschanoff
authors.forename: Jae-Ran
authors.initials: JR
authors.lastname: Lee
authors.forename: Jaewon
authors.initials: J
authors.lastname: Ko
authors.forename: Michel
authors.initials: M
authors.lastname: Streuli
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Weinberg
authors.forename: Morgan
authors.initials: M
authors.lastname: Sheng
authors.forename: Eunjoon
authors.initials: E
authors.lastname: Kim
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001921:Brain / Q000378:metabolism; D007150:Immunohistochemistry; D016547:Kinesins / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding; D051381:Rats; D018091:Receptors, AMPA / Q000378:metabolism; D015398:Signal Transduction; D013347:Subcellular Fractions / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C490575:Kif1a protein, rat; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; C490599:Ppfia4 protein, rat; D018091:Receptors, AMPA; D016547:Kinesins