Main polyol dehydrogenase of Gluconobacter suboxydans IFO 3255, membrane-bound D-sorbitol dehydrogenase, that needs product of upstream gene, sldB, for activity.
The D-sorbitol dehydrogenase gene, sldA, and an upstream gene, sldB, encoding a hydrophobic polypeptide, SldB, of Gluconobacter suboxydans IFO 3255 were disrupted in a check of their biological functions. The bacterial cells with the sldA gene disrupted did not produce L-sorbose by oxidation of D-sorbitol in resting-cell reactions at pHs 4.5 and 7.0, indicating that the dehydrogenase was the main D-sorbitol-oxidizing enzyme in this bacterium. The cells did not produce D-fructose from D-mannitol or dihydroxyacetone from glycerol. The disruption of the sldB gene resulted in undetectable oxidation of D-sorbitol, D-mannitol, or glycerol, although the cells produced the dehydrogenase. The cells with the sldB gene disrupted produced more of what might be signal-unprocessed SldA than the wild-type cells did. SldB may be a chaperone-like component that assists signal processing and folding of the SldA polypeptide to form active D-sorbitol dehydrogenase.
Shinjoh
Masako
M
Department of Applied Microbiology, Nippon Roche Research Center, 200 Kajiwara, Kamakura, Kanagawa 247-8530, Japan. masako.shinjoh@roche.com
Tomiyama
Noribumi
N
Miyazaki
Taro
T
Hoshino
Tatsuo
T
D001483:Base Sequence; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000235:genetics*; D002462:Cell Membrane / Q000201:enzymology; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D020576:Gluconobacter / Q000201:enzymology*; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007064:L-Iditol 2-Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D008773:Methylphenazonium Methosulfate / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013013:Sorbose / Q000096:biosynthesis / Q000737:chemistry; D013402:Sugar Alcohols / Q000378:metabolism
D018797:Cell Cycle Proteins; D004269:DNA, Bacterial; D011994:Recombinant Proteins; D013402:Sugar Alcohols; D008773:Methylphenazonium Methosulfate; D007064:L-Iditol 2-Dehydrogenase; D005947:Glucose; D013013:Sorbose