[Effects of musk glucoprotein on the function of rat polymorphonuclear leukocytes activated by IL-8 in vitro].
OBJECTIVE
To investigate the effects of musk-1, a glucoprotein component isolated from the water extract of musk, on some functions of rat polymorphonuclear leukocytes activated by IL-8 in vitro.


METHODS
An in vitro incubation system was used. Superoxide anion production was determined by cytochrome C reduction. beta-glucuronidase and lysozyme release was quantitated by enzyme reactions in which phenolph-thaleinglucuronic acid and Micrococcus Lysodeikticus were as the substrates, respectively.


RESULTS
In comparison with control, musk-1 at concentration 1-100 micrograms.ml-1 can increase superoxide anion production by 91.7%-291%, and decrease beta-glucuronidase and lysozyme release by 2.2%-58.1% and 3.9%-39.8%, respectively.


CONCLUSIONS
Inhibition of lysosomel enzyme release might be considered as one of mechanisms of antiinflammatory action of musk.
Wang
W J
WJ
Institute of Materia Medica, CAMS and PUMC, Beijing, 100050, China.
Bai
J Y
JY
Cheng
G F
GF
Zhu
X Y
XY
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D005260:Female; D005966:Glucuronidase / Q000378:metabolism*; D008297:Male; D008419:Materia Medica / Q000494:pharmacology*; D009113:Muramidase / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D000894:Anti-Inflammatory Agents, Non-Steroidal; D005229:Fatty Acids, Monounsaturated; D008419:Materia Medica; C008563:musk; D009113:Muramidase; D005966:Glucuronidase; D010100:Oxygen