Application of DNA microarrays in pharmacogenomics and toxicogenomics.
Many drugs or xenobiotics can induce specific or nonspecific cellular signal transduction events that activate various physiologic and pharmacologic responses including homeostasis, proliferation, differentiation, apoptosis, and necrosis. To minimize the insults caused by these xenobiotics, tissues and organs are equipped with protective mechanisms that either pump drugs out of the cells (e.g., the multidrug-resistant, mdr, family of proteins) or increase the level of detoxifying enzymes such as phase I and II drug-metabolizing enzymes (DMEs), after exposure to xenobiotics. This review discusses the molecular analysis of pharmaco- or toxicogenomic gene expression profiles following exposure to cancer chemotherapeutic and chemopreventive agents. We present the development of DNA microarray technology and its use in expression profiling of possible signal transduction events elicited by these compounds, and its potential future applications in drug discovery and development in the pharmaceutical industry.
Chin
Khew-Voon
KV
Department of Medicine and Pharmacology, The Cancer Institute of New Jersey, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, New Brunswick, New Jersey 08901, USA. chinkv@umdnj.edu
Kong
A N Tony
AN
D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods* / Q000639:trends; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000639:trends; D010597:Pharmacogenetics / Q000379:methods* / Q000639:trends