title: Which sites react first? Functional site distribution and kinetics on solid supports investigated using confocal Raman and fluorescence microscopy.
abstract: Fluorescence microscopy is a powerful technique for analyzing beads with very low loadings of fluorophores; however, the method is flawed when looking at more highly loaded beads as a result of severe problems with absorption. To probe distributions at higher loading levels, Raman spectroscopy avoids many of these issues. These studies show that there is a uniform distribution of reactive sites throughout the beads but that the spatial distribution of reacted sites depends on the polymer type, with a fine balance between reaction and diffusion rate.
authors.affiliation: Department of Chemistry, University of Southampton, Southampton SO17 1BJ, United Kingdom.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Kress
authors.forename: Riccardo
authors.initials: R
authors.lastname: Zanaletti
authors.forename: Abigail
authors.initials: A
authors.lastname: Rose
authors.forename: Jeremy G
authors.initials: JG
authors.lastname: Frey
authors.forename: William S
authors.initials: WS
authors.lastname: Brocklesby
authors.forename: Mark
authors.initials: M
authors.lastname: Ladlow
authors.forename: Mark
authors.initials: M
authors.lastname: Bradley
mesh_terms: D001665:Binding Sites; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000379:methods; D008863:Microspheres; D036103:Nanotechnology / Q000379:methods; D012117:Resins, Synthetic; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*
chemical_list: D012117:Resins, Synthetic