[Effect of NF-kappa B pathway on apoptosis of hepatic carcinoma cell line-7721 induced by TNF-alpha].
OBJECTIVE
It was proved that nuclear factor-kappa B play an important role in the activation of immune cell, anti-virus, various stress reaction, and regulation of apoptosis, etc. The objective of this study was to investigate the effect of NF-kappa B signal-transduction pathway on apoptosis of hepatic carcinoma cell line-7721 induced by TNF-alpha.


METHODS
The activation of TNF-alpha on NF-kappa B conduction pathway was determined with ELISA and immunohistochemistry. Then the activation of TNF-alpha was blocked with TPCK. Flow cytometry was used to detect apoptosis.


RESULTS
1. TNF-alpha could activate the NF-kappa B conduction pathway. 2. TPCK could inhibit the activation of NF-kappa B induced by TNF-alpha. 3. Inactivation of NF-kappa B by TPCK could enhance apoptosis induced by TNF-alpha.


CONCLUSIONS
NF-kappa B conduction pathway of 7721 cell takes part in anti-apoptosis and plays an important role in the tolerance on cytotoxicity of TNF-alpha of 7721 cell. Inhibition of NF-kappa B conduction pathway may has potential value in tumor treatment.
Yang
Ji-yun
JY
Department of Clinical Experiment, Chendu Army General Hospital, Chengdu 610083, P. R. China. yangjiyun@yeah.net
Zeng
Xiang-yuan
XY
Ma
Bu-ren
BR
D017209:Apoptosis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha