title: In vivo determination of local skin optical properties and photon path length by use of spatially resolved diffuse reflectance with applications in laser Doppler flowmetry.
abstract: Methods for local photon path length and optical properties estimation, based on measured and simulated diffuse reflectance within 2 mm from the light source, are proposed and evaluated in vivo on Caucasian human skin. The accuracy of the methods was good (2%-7%) for path length and reduced scattering but poor for absorption estimation. Reduced scattering and absorption were systematically lower in the fingertip than in the forearm skin (633 nm). A maximum intrasite and interindividual variation of approximately 35% in an average photon path length was found. The methodology was applied in laser Doppler flowmetry, where path-length normalization of the estimated perfusion removed the optical property dependency.
authors.affiliation: Department of Biomedical Engineering, Linköpings Universitet, University Hospital, S-581 85 Linköping, Sweden. marla@imt.liu.se
authors.forename: Marcus
authors.initials: M
authors.lastname: Larsson
authors.forename: Henrik
authors.initials: H
authors.lastname: Nilsson
authors.forename: Tomas
authors.initials: T
authors.lastname: Strömberg
mesh_terms: D003198:Computer Simulation; D005385:Fingers; D005542:Forearm; D006801:Humans; D017078:Laser-Doppler Flowmetry; D008962:Models, Theoretical; D055095:Optics and Photonics; D017785:Photons; D012039:Regional Blood Flow; D012542:Scattering, Radiation; D012867:Skin / Q000098:blood supply*