IFN-gamma production in rabbits: behavioral and endocrine correlates.
Freely interacting male rabbits were studied to establish the effect of exogenous testosterone on interferon-gamma (IFN-gamma) production in peripheral blood mononuclear cells (PBMCs) and to evaluate if this effect is related to season, social rank, plasma corticosterone and glucocorticoid receptors (GcR) in PBMCs. Dominance behavior increases after testosterone propionate (TP) administration only in rank 1 animals, while submission behavior increases after TP only in rank 4 animals, indicating a reinforcing effect of TP on the behavior. Corticosterone and IFN-gamma production are higher and GcR binding capacity is lower in spring than in autumn, suggesting that seasonal fluctuations in the immune system may be related to the pattern of secretion of immunomodulatory hormones. In autumn, corticosterone decreases after TP treatment and increases after social interaction, while GcR binding capacity decreases after TP treatment and social interaction. IFN-gamma production decreases in spring and increases in autumn after TP treatment plus social interaction, indicating that the modulating action of testosterone is related to the current immune status. The relationship between dominance, testosterone and the immune system in spring is suggested by the finding that GcR binding capacity after TP treatment is directly related to social rank, as confirmed by the positive correlation with dominance behavior frequency. The dominance index is positively correlated with GcR binding capacity and negatively with IFN-gamma production before TP treatment, indicating that high receptor activity in immunocompetent cells and low immunoreactivity could be prerequisites for dominance behavior. The immunosuppressive effect of corticosterone and the mechanism of down-regulation on GcR are confirmed by the negative correlations with IFN-gamma production and GcR binding capacity.
Muscettola
Michela
M
Department of Physiology, University of Siena, Via Aldo Moro, I-53100 Siena, Italy. muscettola@unisi.it
Massai
Lauretta
L
Lodi
Leda
L
Briganti
Federica
F
Fontani
Giuliano
G
Lupo
Concetta
C
D000818:Animals; D001522:Behavior, Animal; D002478:Cells, Cultured; D003345:Corticosterone / Q000097:blood; D007109:Immunity; D007371:Interferon-gamma / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D011817:Rabbits; D011965:Receptors, Glucocorticoid / Q000097:blood; D012621:Seasons; D012930:Social Dominance; D013739:Testosterone / Q000494:pharmacology*
D011965:Receptors, Glucocorticoid; D013739:Testosterone; D007371:Interferon-gamma; D003345:Corticosterone