Identification of coronaviruses in dogs that segregate separately from the canine coronavirus genotype.
The genetic diversity of 16 canine coronavirus (CCoV) samples is described. Samples were obtained from pups infected naturally living in different areas. Sequence data were obtained from the M gene and pol1a and pol1b regions. The phylogenetic relationships among these sequences and sequences published previously were determined. The canine samples segregated in two separate clusters. Samples of the first cluster were intermingled with reference strains of CCoV genotype and therefore could be assigned to this genotype. The second cluster segregated separately from CCoV and feline coronavirus genotypes and therefore these samples may represent genetic outliers. The reliability of the classification results was confirmed by repeating the phylogenetic analysis with nucleotide and amino acid sequences from multiple genomic regions.
Pratelli
Annamaria
A
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010, Valenzano, Bari, Italy. a.pratelli@veterinaria.uniba.it
Martella
Vito
V
Pistello
Mauro
M
Elia
Gabriella
G
Decaro
Nicola
N
Buonavoglia
Domenico
D
Camero
Michele
M
Tempesta
Maria
M
Buonavoglia
Canio
C
D000595:Amino Acid Sequence; D000818:Animals; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D000086862:Coronavirus M Proteins; D017939:Coronavirus, Canine / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014763:Viral Matrix Proteins / Q000235:genetics
D000086862:Coronavirus M Proteins; D014763:Viral Matrix Proteins; D012321:DNA-Directed RNA Polymerases