Rapid microsatellite analysis using discontinuous polyacrylamide gel electrophoresis.
A method for screening large numbers of samples for microsatellites using discontinuous, non-denaturing polyacrylamide gels and rapid fluorescent gel staining is described. Disc electrophoresis on slab gels provides high-resolution of PCR products. It is useful for collecting population data once microsatellite loci have been characterized.
White
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E
Pacific Forestry Centre, Canadian Forest Service, 506 West Burnside Road, Victoria, BC V8Z 1M5, Canada. ewhite@pfc.forestry.ca
Sahota
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R
Edes
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S
D001483:Base Sequence; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction
D017931:DNA Primers