[Mutation analysis of KLF6 gene in human nasopharyngeal carcinomas].
OBJECTIVE
Nasopharyngeal carcinoma (NPC) is one of the most common cancers in South China and Southeast Asia. The etiological factor is believed to be the interaction between genetic susceptibility, EBV infection and environmental factors, involved in the multi-step process of carcinogenesis and development of NPC. However, the molecular pathology of NPC is unclear yet. Kruppel-like factor 6(KLF6) is a ubiquitously expressed nuclear transcription factor, which is deleted and/or mutated with high frequency in a subset of prostate cancer. This study was designed to investigate KLF6 mutation in NPC tumors and NPC cell lines.


METHODS
Genomic DNAs from 19 NPC tumor biospies and 3 NPC cell lines were used in mutation detection of KLF6 coding region and splice sites by PCR-sequencing. 100 chromosomes from 50 random healthy individuals were used as control.


RESULTS
In 3 of 19 NPC tissues, 3 different mutations (Glu75Val, Ser136Arg, Arg243 Lys) in KLF6 gene were found by PCR-sequencing. None of the 3 mutations were detected in the 50 random healthy individuals.


CONCLUSIONS
KLF6 gene may be involved in carcinogenesis of sporadic NPC.
Chen
Han-kui
HK
Cancer Center, Sun Yat-sen University, Guangzhou 510060, P. R. China.
Liu
Xiao-qiong
XQ
Lin
Jie
J
Chen
Tian-yu
TY
Feng
Qi-sheng
QS
Zeng
Yi-xin
YX
D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004252:DNA Mutational Analysis; D006801:Humans; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors; D009154:Mutation; D020125:Mutation, Missense; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators / Q000235:genetics*
C105465:KLF6 protein, human; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D004247:DNA