Effect of genotype on steady-state CYP1A1 gene expression in human peripheral lymphocytes.
We have analyzed the steady-state levels of cytochrome P-450 1A1 (CYP1A1) mRNA in peripheral blood lymphocytes of 177 individuals with various CYP1A1 genotypes using a quantitative reverse transcriptase-polymerase chain reaction technique that makes use of a homologous internal standard for accurate quantitation. We found no effects of ethnicity, age, or smoking status on CYP1A1 gene expression in this population. We did see a significant 2-fold increase in the mean level of CYP1A1 mRNA in women compared with men for both Caucasians and African Americans. We observed no effect of the African American-specific polymorphism (CYP1A1(*)3) on expression of the gene. However, we found a significant 3-fold decrease in expression associated with the homozygous MspI restriction fragment length polymorphism (CYP1A1(*)2A/(*)2A).
Garte
Seymour
S
EOHSI-UMDNJ, 170 Frelinghuysen Road, Piscataway, NJ 08854, USA. garte@eoshi.rutgers.edu
Ganguly
Sabya
S
Taioli
Emanuela
E
D001741:Black or African American; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D005838:Genotype; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000201:enzymology / Q000378:metabolism*; D011110:Polymorphism, Genetic; D020871:RNA Stability; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D019363:Cytochrome P-450 CYP1A1