Fabrication of a novel porous PGA-chitosan hybrid matrix for tissue engineering.
Polyglycolide (PGA) and chitosan mixture solution was prepared using solvents of low toxicity to create novel, porous, biocompatible, degradable, and modifiable hybrid matrices for biomedical applications. The porosity of these PGA-chitosan hybrid matrices (P/C matrices) was created by a thermally induced phase separation method. Two types of the P/C hybrid matrices containing 70 wt% PGA (P/C-1 matrix) and 30 wt% PGA (P/C-2 matrix) were fabricated. Chitosan matrix was also prepared for comparison. A 35-day in vitro degradation revealed that the weight losses for the P/C-1 and P/C-2 matrices were similar ( approximately 61%), but the releases of glycolic acid from the P/C-1 and P/C-2 matrices were 95% and 60%, respectively. The P/C-1 matrix had higher porosity and higher mechanical strength than the P/C-2 and chitosan matrices. Fibroblast cells cultivated in these matrices proliferated well and the cell density was the highest in the P/C-1 matrix, followed by the chitosan and P/C-2 matrices, suggesting good biocompatibility for the P/C-1 matrix. We thereby concluded that the P/C-1 matrix, due to its high strength, porosity, biocompatibility and degradability, is a promising biomaterial. The presence of chitosan in the P/C matrices provides many amino groups for further modifications such as biomolecule conjugation and thus enhances the application potential of the P/C hybrid matrices in tissue engineering.
Wang
Yu Chi
YC
Department of Chemical Engineering, National Taiwan University, 106, Taipei, Taiwan, ROC.
Lin
Mei Chiao
MC
Wang
Da Ming
DM
Hsieh
Hsyue Jen
HJ
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D018929:Cell Culture Techniques / Q000379:methods; D002470:Cell Survival / Q000187:drug effects; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000648:ultrastructure; D048271:Chitosan; D005347:Fibroblasts / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D011100:Polyglycolic Acid / Q000737:chemistry*; D016062:Porosity; D011208:Powders; D023822:Tissue Engineering / Q000379:methods
D001672:Biocompatible Materials; D011208:Powders; D002686:Chitin; D011100:Polyglycolic Acid; D048271:Chitosan