[DNA cloning of Ad7 vaccine strain and sequence analysis of its Sma I H fragment].
Human adenoviruses have been a hot topic of study because of their potential utility for gene therapy and development of live viral vectored vaccines. Adenovirus types 4 and 7 have been given orally as vaccines for prevention of acute respiratory infections caused by these serotypes of adenovirus in U.S. army recruits for more than 30 years. Therefore, the advantage of using Ad4 and Ad7 as vectors in the development of oral live recombinant vaccine is obvious. For constructing Ad7 vector, 10.8-100 mu DNA of Ad7 vaccine strain has been cloned. The Sma I H fragment was sequenced using Ad7 DNA as a template and then the fragment was cloned by PCR. For verifying the sequence the fragment was sequenced again. The strategy we used to clone terminal end of adenovirus is reliable and practicable.
Zhou
J
J
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052.
Qu
J
J
Shi
C
C
D017503:Adenovirus E3 Proteins / Q000235:genetics* / Q000276:immunology; D000260:Adenoviruses, Human / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D053938:DNA Fragmentation; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D015723:Gene Library; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014614:Vaccines, Synthetic
D017503:Adenovirus E3 Proteins; D004274:DNA, Recombinant; D004279:DNA, Viral; C084390:E3 glycoprotein 19k, Adenovirus type 7; D014614:Vaccines, Synthetic