title: Identification of trypanosomes in wild animals from southern Cameroon using the polymerase chain reaction (PCR).
abstract: One possible explanation of the maintenance of many historical foci of sleeping sickness in Central Africa could be the existence of a wild animal reservoir. In this study, PCR was used to detect the different trypanosome species present in wild animal captured by hunters in the southern forest belt of Cameroon (Bipindi). Trypanosomes were also detected by a parasitological method (Quantitative buffy coat: QBC). Parasite could not be isolated in culture medium (Kit for in vitro isolation: KIVI). Specific primers of T. brucei s.l., T. congolense forest type, T. congolense savannah type, T. vivax, T. simiae and T. b. gambiense group 1 were used to identify parasites in the blood of 164 animals belonging to 24 different species including ungulates, rodents, pangolins, carnivores, reptiles and primates. Of the 24 studied species, eight were carrying T. b. gambiense group 1. Those parasites pathogenic to man were found in monkeys (Cercocebus torquatus and Cercopithecus nictitans), in ungulates (Cephalophus dorsalis and C. monticola), in carnivores (Nandinia binotata and Genetta servalina) and in rodents (Cricetomys gambianus and Atherurus africanus). 13 species (54%) were carrying T. brucei s.l. identified as non-gambiense group 1.
authors.affiliation: LRCT IRD/CIRAD, TA 207/G, Campus international de Baillarguet, F-34398 Montpellier, France. herder@mpl.ird.fr
authors.forename: S
authors.initials: S
authors.lastname: Herder
authors.forename: G
authors.initials: G
authors.lastname: Simo
authors.forename: S
authors.initials: S
authors.lastname: Nkinin
authors.forename: F
authors.initials: F
authors.lastname: Njiokou
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000469:parasitology*; D002163:Cameroon; D016054:DNA, Protozoan / Q000302:isolation & purification*; D004197:Disease Reservoirs / Q000662:veterinary; D007303:Insect Vectors / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D013045:Species Specificity; D014345:Trypanosoma / Q000235:genetics / Q000302:isolation & purification*; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000302:isolation & purification; D014347:Trypanosoma brucei gambiense / Q000235:genetics / Q000302:isolation & purification; D014348:Trypanosoma congolense / Q000235:genetics / Q000302:isolation & purification; D014352:Trypanosomiasis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*
chemical_list: D016054:DNA, Protozoan