title: [Effect of NF-kappa B pathway on apoptosis of hepatic carcinoma cell line-7721 induced by TNF-alpha].
abstract: OBJECTIVE
  It was proved that nuclear factor-kappa B play an important role in the activation of immune cell, anti-virus, various stress reaction, and regulation of apoptosis, etc. The objective of this study was to investigate the effect of NF-kappa B signal-transduction pathway on apoptosis of hepatic carcinoma cell line-7721 induced by TNF-alpha.
  
  
  METHODS
  The activation of TNF-alpha on NF-kappa B conduction pathway was determined with ELISA and immunohistochemistry. Then the activation of TNF-alpha was blocked with TPCK. Flow cytometry was used to detect apoptosis.
  
  
  RESULTS
  1. TNF-alpha could activate the NF-kappa B conduction pathway. 2. TPCK could inhibit the activation of NF-kappa B induced by TNF-alpha. 3. Inactivation of NF-kappa B by TPCK could enhance apoptosis induced by TNF-alpha.
  
  
  CONCLUSIONS
  NF-kappa B conduction pathway of 7721 cell takes part in anti-apoptosis and plays an important role in the tolerance on cytotoxicity of TNF-alpha of 7721 cell. Inhibition of NF-kappa B conduction pathway may has potential value in tumor treatment.
authors.affiliation: Department of Clinical Experiment, Chendu Army General Hospital, Chengdu 610083, P. R. China. yangjiyun@yeah.net
authors.forename: Ji-yun
authors.initials: JY
authors.lastname: Yang
authors.forename: Xiang-yuan
authors.initials: XY
authors.lastname: Zeng
authors.forename: Bu-ren
authors.initials: BR
authors.lastname: Ma
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha