[Immunoscreening of Schistosoma japonicum adult worm cDNA library by sera from rabbits vaccinated with ultraviolet-attenuated cercariae].
OBJECTIVE
To find out the major antigenic molecules of radiation-attenuated cercariae, and provide some useful candidate antigens for developing schistosomiasis vaccine.


METHODS
Schistosoma japonicum (Sj) adult worm cDNA library was screened by sera of rabbits vaccinated with ultraviolet-attenuated cercariae, and the inserts of positive clones were specifically amplified by PCR and sequenced.


RESULTS
Ten positive clones were obtained after three rounds of screening, and the size of Sj cDNA fragments of the positive clones ranged from 1.5 kb to 1.8 kb. Five partial sequences were got after preliminary sequencing. Two of them had significantly homology with Sj dynein light chain 5(DLC 5) gene and Sj mitochondrial gene, respectively, and the others were identified as partial sequences of novel genes for they showed only partial homology with non-schistosome genes or other organism in the database.


CONCLUSIONS
The positive clones may be the genes encoding the antigens that can elicit protective immunity against Sj.
Zhou
D M
DM
Laboratory of Schistosomiasis, Hunan Medical University, Changsha 410078.
Yi
X Y
XY
Zeng
X F
XF
Zhou
J C
JC
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D015723:Gene Library; D007106:Immune Sera / Q000235:genetics* / Q000276:immunology; D007814:Larva / Q000276:immunology / Q000528:radiation effects; D011817:Rabbits; D012549:Schistosoma japonicum / Q000235:genetics* / Q000276:immunology; D012554:Schistosomiasis japonica / Q000517:prevention & control; D014466:Ultraviolet Rays; D014613:Vaccines, Attenuated / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*
D000947:Antigens, Helminth; D007106:Immune Sera; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA