[Change of adhesion molecule expression on CD34(+) cells from bone marrow and peripheral blood during mobilization with combination of chemotherapy and G-CSF].
To explore the dynamic change of CD34(+) cell expressing adhesion molecules in bone marrow and peripheral blood during mobilization with combination of chemotherapy and G-CSF and its clinical significance, mononuclear cells of bone marrow and peripheral blood from malignant hematopathy cases before and after mobilization with G-CSF were labeled by CD45-CY-Chrome, PE conjugated anti-CD34, and FITC conjugated anti-CD44, anti-CD49d, anti-CD62L and anti-CXCR4. For three-color fluorescence analysis by flow cytometry was performed on a FACScalibur. Also the relationship between the number of subpopulations in different expressions of adhesion molecules infused and the time of recovery in different blood cells after transplantation was evaluated. Results showed that a significantly lower expression of CD44(+) and CD49d(+) on CD34(+) cells in bone marrow after mobilization compared to that before mobilization, whereas great higher expression of CD44(+), CD49d(+), anti-CD62L(+) and lower of anti-CXCR4(+) in peripheral blood were observed after mobilization. No significant relations were found between expression of different adhesion molecules on CD34(+) cells infused and the time of reconstitution in blood cells after transplantation. It was concluded that this mobilizing regimen could downregulate the expressions of CD44, CD49d, CD62L, and anti-CXCR4 on CD34(+) cells in bone marrow, it may related to mobilization of CD34(+) cells from marrow to blood, and homing of blood CD34(+) cells into marrow.
Da
Wan-Ming
WM
Department of Hematology, The General Hospital of PLA, Beijing 100853, China. DWanming@hotmail.com
Zhang
Miao
M
Zhang
Bo-Long
BL
Jin
Hai-Jie
HJ
Yu
Li
L
Han
Xiao-Ping
XP
Jing
Yu
Y
Zhao
Yu
Y
Wu
Xiao-Xiong
XX
Huang
Wen-Rong
WR
Wang
Quan-Shun
QS
D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000276:immunology*; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000097:blood; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use; D019337:Hematologic Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000628:therapy; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation; D006689:Hodgkin Disease / Q000276:immunology / Q000378:metabolism / Q000628:therapy; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D039441:Integrin alpha4 / Q000096:biosynthesis; D019041:L-Selectin / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism*; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology / Q000378:metabolism / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000276:immunology / Q000378:metabolism / Q000628:therapy; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000378:metabolism / Q000628:therapy
D018952:Antigens, CD34; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D019041:L-Selectin; D016179:Granulocyte Colony-Stimulating Factor; D039441:Integrin alpha4