title: Pseudomonas extremorientalis sp. nov., isolated from a drinking water reservoir.
abstract: On the basis of phenotypic and genotypic characteristics and 16S rDNA sequence analysis, a novel species belonging to the genus Pseudomonas sensu stricto was identified. The saprophytic, fluorescent bacterium, designated KMM 3447(T), was isolated from a drinking water reservoir near Vladivostok City, Russia. The novel organism was a Gram-negative, aerobic, rod-shaped bacterium that produced a cyclic depsipeptide with surface-active properties. It degraded casein, but did not degrade gelatin, starch, agar or Tween 80. The bacterium was also haemolytic. Growth of the novel bacterium occurred between 4 and 35 degrees C. The predominant cellular fatty acids of the novel pseudomonad were C16:0, C16:1(n-7), C18:1(n-7) and C17.0 cyclo; branched fatty acids were only found in trace amounts. The G+C content of the novel bacterium was 61.0 mol%. 16S rDNA sequence analysis indicated that the novel bacterium had a clear affiliation with Pseudomonas fluorescens and species closely related to this recognized pseudomonad. DNA-DNA hybridization experiments showed that the novel bacterium bound at low levels (27-53%) with the DNA of the type strains of its nearest phylogenetic relatives, namely Pseudomonas tolaasii, Pseudomonas veronii, Pseudomonas orientalis and Pseudomonas rhodesiae, indicating that the novel bacterium represented a novel species within the genus Pseudomonas, for which the name Pseudomonas extremorientalis is proposed; the type strain is KMM 3447(T) (= LMG 19695(T)).
authors.affiliation: Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russia. eivanova@swin.edu.au
authors.forename: Elena P
authors.initials: EP
authors.lastname: Ivanova
authors.forename: Nataliya M
authors.initials: NM
authors.lastname: Gorshkova
authors.forename: Tomoo
authors.initials: T
authors.lastname: Sawabe
authors.forename: Karin
authors.initials: K
authors.lastname: Hayashi
authors.forename: Nataliya I
authors.initials: NI
authors.lastname: Kalinovskaya
authors.forename: Anatolii M
authors.initials: AM
authors.lastname: Lysenko
authors.forename: Natalie V
authors.initials: NV
authors.lastname: Zhukova
authors.forename: Dan V
authors.initials: DV
authors.lastname: Nicolau
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Kuznetsova
authors.forename: Valery V
authors.initials: VV
authors.lastname: Mikhailov
authors.forename: Richard
authors.initials: R
authors.lastname: Christen
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D013045:Species Specificity; D014871:Water Microbiology; D014881:Water Supply
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S