Role of luminal nutrients and endogenous GLP-2 in intestinal adaptation to mid-small bowel resection.
To elucidate the role of luminal nutrients and glucagon-like peptide-2 (GLP-2) in intestinal adaptation, rats were subjected to 70% midjejunoileal resection or ileal transection and were maintained with total parenteral nutrition (TPN) or oral feeding. TPN rats showed small bowel mucosal hyperplasia at 8 h through 7 days after resection, demonstrating that exogenous luminal nutrients are not essential for resection-induced adaptation when residual ileum and colon are present. Increased enterocyte proliferation was a stronger determinant of resection-induced mucosal growth in orally fed animals, whereas decreased apoptosis showed a greater effect in TPN animals. Resection induced significant transient increases in plasma bioactive GLP-2 during TPN, whereas resection induced sustained increases in plasma GLP-2 during oral feeding. Resection-induced adaptive growth in TPN and orally fed rats was associated with a significant positive correlation between increases in plasma bioactive GLP-2 and proglucagon mRNA expression in the colon of TPN rats and ileum of orally fed rats. These data support a significant role for endogenous GLP-2 in the adaptive response to mid-small bowel resection in both TPN and orally fed rats.
Dahly
Elizabeth M
EM
Department of Nutritional Sciences, University of Wisconsin, Madison 53706, USA.
Gillingham
Melanie B
MB
Guo
Ziwen
Z
Murali
Sangita G
SG
Nelson
David W
DW
Holst
Jens J
JJ
Ney
Denise M
DM
D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001835:Body Weight; D002455:Cell Division / Q000502:physiology; D004435:Eating / Q000502:physiology; D020895:Enterocytes / Q000166:cytology; D005934:Glucagon / Q000378:metabolism; D053767:Glucagon-Like Peptide 2; D004763:Glucagon-Like Peptides; D007082:Ileum / Q000166:cytology / Q000502:physiology / Q000601:surgery*; D007583:Jejunum / Q000166:cytology / Q000502:physiology / Q000601:surgery*; D008297:Male; D010288:Parenteral Nutrition; D010455:Peptides / Q000378:metabolism*; D052336:Proglucagon; D011498:Protein Precursors / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013393:Sucrase / Q000378:metabolism
D053767:Glucagon-Like Peptide 2; D010455:Peptides; D011498:Protein Precursors; D052336:Proglucagon; D004763:Glucagon-Like Peptides; D005934:Glucagon; D013393:Sucrase