The effect of nickel sulphate, potassium dichromate, cobalt nitrate and cadmium sulphate on the proteins of cellular contacts and actin skeleton of cultivated human keratinocytes.
The authors tested the effects of known allergens, namely nickel sulphate, potassium dichromate, cobalt nitrate and cytotoxic cadmium sulphate on the proteins of cellular contacts (vinculin, talin, E-cadherin, desmoplaktin) and actin cytoskeleton (actin filaments) of cultivated human keratinocytes. The localisation of proteins of cellular contacts was detected by means of direct immunofluorescence. The authors have detected a decrease in, and destruction of cellular contact proteins and actin cytoskeleton after testing the effect of all allergens, while the most significant changes were detected in E-cadherin, vunkulin and actin filaments. Desmoplaktin and talin were less damaged. Potassium dichromate caused damage already in concentration of 1 microg/ml. A similar effect of the other two tested haptanes was brought about in concentration being 100-fold higher.


CONCLUSIONS
the gained results indicate that the investigation of cellular contact proteins and actin cytoskeleton of cultivated human keratinocytes can possibly become a part of the testing of allergy-triggering potential of chemical substances. (Tab. 1, Fig. 4, Ref. 18.)
Lozsekova
A
A
1st Department of Dermatovenerology, Faculty of Medicine, Comenius University Bratislava, Slovakia. bll@fmed.uniba.sk
Kaiser
H W
HW
Danilla
T
T
Buchvald
J
J
Simko
J
J
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000485:Allergens / Q000494:pharmacology*; D019187:Cadmium Compounds / Q000494:pharmacology; D002478:Cells, Cultured; D003035:Cobalt / Q000494:pharmacology; D003598:Cytoskeletal Proteins / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007365:Intercellular Junctions / Q000187:drug effects* / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D008670:Metals / Q000494:pharmacology*; D009532:Nickel / Q000494:pharmacology; D011192:Potassium Dichromate / Q000494:pharmacology; D013431:Sulfates / Q000494:pharmacology
D000485:Allergens; D019187:Cadmium Compounds; D003598:Cytoskeletal Proteins; D008670:Metals; D013431:Sulfates; D003035:Cobalt; C029938:nickel sulfate; C025913:cobaltous nitrate; D009532:Nickel; C037123:cadmium sulfate; D011192:Potassium Dichromate