title: In ovo electroporation of Crim1 in the developing chick spinal cord.
abstract: The novel mammalian gene Crim1 encodes a transmembrane bound protein with similarity to the secreted bone morphogenetic protein (BMP) antagonists, vertebrate Chordin, and its Drosophila homologue short gastrulation. Crim1 is expressed in the neural tube in mouse in a restricted pattern, but its function in central nervous system development is largely unknown. We isolated the chicken Crim1 orthologue and analyzed its expression in the developing neural tube. Chicken CRIM1 shares strong homology to human/mouse CRIM1 and C. elegans CRIM1-like proteins. Crim1 is expressed in a similar but not identical pattern to that in the developing spinal cord of mouse, including the notochord, floor plate, motor neurons, and the roof plate. Unlike follistatin, a secreted inhibitor of BMPs, in ovo electroporation of CRIM1, as a full-length transmembrane bound or secreted ectodomain was not sufficient to disrupt early patterning of the neural tube. However, ectodomain CRIM1 overexpression leads to an approximate 50% decrease in populations of specific ventral neuronal populations, including ISL-1(+) motor neurons, CHX-10(+) V1, and EN-1(+) V2 interneurons.
authors.affiliation: Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Kolle
authors.forename: Annemieke
authors.initials: A
authors.lastname: Jansen
authors.forename: Toshiya
authors.initials: T
authors.lastname: Yamada
authors.forename: Melissa
authors.initials: M
authors.lastname: Little
mesh_terms: D000818:Animals; D052004:Bone Morphogenetic Protein Receptors; D017173:Caenorhabditis elegans; D002490:Central Nervous System / Q000196:embryology; D002642:Chick Embryo; D003001:Cloning, Molecular; D018274:Electroporation; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins; D051379:Mice; D009046:Motor Neurons / Q000166:cytology; D009432:Neural Crest / Q000196:embryology; D009474:Neurons / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011506:Proteins; D013116:Spinal Cord / Q000196:embryology*
chemical_list: C486175:CRIM1 protein, human; D008565:Membrane Proteins; D009687:Nuclear Proteins; D011506:Proteins; C074370:cysteine-rich protein, mammalian; D052004:Bone Morphogenetic Protein Receptors