Uses and limitations of the XTT assay in studies of Candida growth and metabolism.
Colorimetric tetrazolium assays are used increasingly in studies of fungi, often in the absence of standardization or correlation with other methods. We examined species- and strain-related tetrazolium metabolism in Candida albicans and Candida parapsilosis by using XTT [2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide] and WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphonyl)-2H-tetrazolium] and found marked variations. Also, significant signal was often missed in the absence of dimethyl sulfoxide extraction.
Kuhn
D M
DM
Division of Infectious Diseases, Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Balkis
M
M
Chandra
J
J
Mukherjee
P K
PK
Ghannoum
M A
MA
D002175:Candida / Q000254:growth & development* / Q000378:metabolism; D002455:Cell Division; D003124:Colorimetry / Q000379:methods; D006801:Humans; D013778:Tetrazolium Salts / Q000378:metabolism
D013778:Tetrazolium Salts; C042207:4-anisyltetrazolium blue