[Use of diagnostic DNA probes for detection of the provirus of bovine leukemia virus in infected cows].
A method of detection of BLV proviral DNA in the peripheral blood leukocytes of cattle is reported. Leukocytes were used without preliminary cultivation. Cell DNA was dot-hybridized with P-labeled plasmid containing a fragment of BLV proviral DNA. The sera samples taken from the cattle under study were also tested using immunodiffusion assay (ID). The results of comparison showed that dot-hybridization assay is a more sensitive diagnostic test than ID, because it detects BLV infection in apparently normal cattle, which was seronegative in ID.
Mal'tseva
N A
NA
Kaledin
A S
AS
Oliĭnik
E I
EI
Zhivotov
A A
AA
Baranov
V I
VI
Karelin
V P
VP
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D016583:Enzootic Bovine Leukosis / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D015151:Immunoblotting / Q000379:methods; D005779:Immunodiffusion; D001909:Leukemia Virus, Bovine / Q000235:genetics / Q000302:isolation & purification*; D007962:Leukocytes / Q000821:virology*; D009693:Nucleic Acid Hybridization; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D015342:DNA Probes; D004279:DNA, Viral