[Establishment of a method for detecting anti-HCV IgM using recombinated structural region antigens].
Presence of IgM antibody to viral structural protein can be taken as the marker of its replication, so the detection method for anti-HCV IgM was established using as HCV recombinated structural region antigens, including C, E1, E2, as the coated antigen and rabbit anti-human gamma chain sera for neutralization and sheep anti-rabbit IgG coated for the sorbence of the complex of RAH-gamma with human IgG. 76 serum specimens of the hepatitis C patients were detected by indirect ELISA for anti-HCV IgM and by RT-PCR for RNA. The results suggested that IgM antibodies to C, E1, E2 were closely related with RNA (P < 0.005). The components of the IgM antibody, too were identified by indirect ELISA with recombined antigens coated respectively. When whole-fragment C or C + E2 were used as the coated antigens, anti-HCV IgM in 96.6% or 100% of serum specimens can be respectively detected. Using recombined structural region antigens, detection of anti-HCV IgM by ELISA was high sensitivity and specificity.
Wang
J
J
Beijing Youan Hospital, 100054.
Min
F
F
Zhang
S
S
D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000096:biosynthesis* / Q000276:immunology; D005814:Genes, Viral / Q000235:genetics / Q000276:immunology; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology*; D006526:Hepatitis C / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015678:Viral Structural Proteins / Q000235:genetics; D014779:Virus Replication
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007075:Immunoglobulin M; D011994:Recombinant Proteins; D015678:Viral Structural Proteins