title: A simple method for preparation of D-rhamnose.
abstract: A rapid procedure for the preparation of D-rhamnose from bacterial lipopolysaccharide (LPS) has been developed. It involves purification of LPS from Pseudomonas syringae pv. phaseolicola by phenol extraction and hydrophobic interaction chromatography (HIC), followed by mild hydrolysis and cleavage of the O-antigen into D-fucose and D-rhamnose. The monosaccharides were separated by column chromatography, and D-rhamnose recovered after filtration over Sephadex-LH 20.
authors.affiliation: Institute of Pharmacy, Friedrich-Schiller-University Jena, Semmelweisstrasse 10, D-07743 Jena, Germany. mramm@pmail.hki-jena.de
authors.forename: Michael
authors.initials: M
authors.lastname: Ramm
authors.forename: Martina
authors.initials: M
authors.lastname: Lobe
authors.forename: Matthias
authors.initials: M
authors.lastname: Hamburger
mesh_terms: D002845:Chromatography; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification; D011549:Pseudomonas / Q000737:chemistry; D012210:Rhamnose / Q000138:chemical synthesis* / Q000302:isolation & purification
chemical_list: D008070:Lipopolysaccharides; D012210:Rhamnose