[Effects of deoxynivalenol on proliferation and tumor necrosis factor-alpha secretion of human peripheral blood mononuclear cells in vitro].
Deoxynivalenol (DON) is one of the most common contaminating mycotoxins in food at high risk areas of esophageal carcinoma in China. The effects of DON on proliferation and tumor necrosis factor-alpha (TNF-alpha) secretion of human peripheral blood mononuclear (HPBM) cells in vitro were determined with flow cytometric (FCM) analysis, cell counting, MTT bioassay and ELISA method respectively. FCM analysis revealed that after HPBM were prestimulated with PHA for 48 h, the proliferation indexes(PI) of the cells treated with low concentration of DON (50-500 ng/ml) for 6 h were all dramatically lower than that of normal control (19.80%-26.01% vs 37.84%), while the PI of cells treated with high concentration of DON(1000-2000 ng/ml) for 24 h were higher than that of normal control (35.74%-34.37% vs 22.85%). Cell number counting and MTT bioassay results showed that at the presence of PHA, DON inhibited the proliferation of HPBM. Double antibody sandwich enzyme linked immunosorbent assay (ELISA) showed that the secretion of TNF-alpha in cells treated with DON(500-1000 ng/ml) was significantly lower than that in the control (P < 0.01). The results suggested that DON could either inhibit or stimulate cell proliferation depending on active time, concentration and PHA stimulation. In general, DON mainly inhibited the proliferation of HPBM and decrease TNF-alpha secretion in vitro. Thus, DON might have some negative impacts on human immunological system.
Wang
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H
Lab of Experimental Pathology, Hebei Medical Unviersity, Shijiazhuang 050017, China.
Zhang
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X
Yang
Y
Y
Yan
X
X
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects*; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000502:physiology; D009183:Mycotoxins / Q000009:adverse effects; D014255:Trichothecenes / Q000009:adverse effects*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D007166:Immunosuppressive Agents; D009183:Mycotoxins; D014255:Trichothecenes; D014409:Tumor Necrosis Factor-alpha; C007262:deoxynivalenol