title: Delayed hypothermia prevents decreases in N-acetylaspartate and reduced glutathione in the cerebral cortex of the neonatal pig following transient hypoxia-ischaemia.
abstract: The effects of normothermia and delayed hypothermia on the levels of N-acetylaspartate (NAA), reduced glutathione (GSH) and the activities of mitochondrial complex I, II-III, IV and citrate synthase were measured in brain homogenates obtained from anaesthetized neonatal pigs following transient in vivo hypoxia-ischaemia. In the normothermic animals there was a significant decrease in complex I activity and in the levels of GSH and NAA when compared to the controls. Delayed hypothermia preserved NAA and GSH at control levels and enhanced the rate of complex II-III activity. There was correlation (R = 0.79) between GSH and NAA levels when data from all three experimental groups were analyzed. Citrate synthase activity was not significantly different in the three groups, indicating maintenance of mitochondrial integrity. These data suggest that delayed hypothermia affords protection of integrated mitochondrial function in the neonatal brain following transient hypoxia-ischaemia.
authors.affiliation: Department of Paediatrics, University College London Medical School, London WC1E 6JJ, United Kingdom. Keith_J_Brooks@gsk.com
authors.forename: Keith J
authors.initials: KJ
authors.lastname: Brooks
authors.forename: Iain
authors.initials: I
authors.lastname: Hargreaves
authors.forename: Kishore
authors.initials: K
authors.lastname: Bhakoo
authors.forename: Mark
authors.initials: M
authors.lastname: Sellwood
authors.forename: Francis
authors.initials: F
authors.lastname: O'Brien
authors.forename: Martina
authors.initials: M
authors.lastname: Noone
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Sakata
authors.forename: Ernest
authors.initials: E
authors.lastname: Cady
authors.forename: Marzena
authors.initials: M
authors.lastname: Wylezinska
authors.forename: John
authors.initials: J
authors.lastname: Thornton
authors.forename: Roger
authors.initials: R
authors.lastname: Ordidge
authors.forename: Quyen
authors.initials: Q
authors.lastname: Nguyen
authors.forename: Matthew
authors.initials: M
authors.lastname: Clemence
authors.forename: John
authors.initials: J
authors.lastname: Wyatt
authors.forename: Timothy E
authors.initials: TE
authors.lastname: Bates
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000378:metabolism*; D002540:Cerebral Cortex / Q000201:enzymology / Q000378:metabolism*; D002950:Citrate (si)-Synthase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D007035:Hypothermia / Q000378:metabolism*; D020925:Hypoxia-Ischemia, Brain / Q000201:enzymology / Q000378:metabolism*; D013552:Swine
chemical_list: D001224:Aspartic Acid; C000179:N-acetylaspartate; D002950:Citrate (si)-Synthase; D005978:Glutathione