title: Induction of cellular immunity to varicella-zoster virus glycoproteins tested with pernasal coadministration of Escherichia coli enterotoxin in mice.
abstract: A mutant of Escherichia coli enterotoxin promotes the induction of cellular immunity to a live varicella vaccine (the Oka strain) as a mucosal adjuvant in mice. An investigation was carried out to determine which of the purified glycoproteins of the virus among three induced cellular immunity with a single nasal administration. Spleen cells from mice immunized nasally with the vaccine and toxin produced interleukin-2 (IL-2) at the same level on restimulation in vitro with glycoprotein H: glycoprotein L (gH:gL), gB, and gE:gI, but not IL-4. The spleen cells from mice immunized with gH:gL, gB, or gE:gI and toxin produced IL-2 on restimulation with gH:gL, gB, or gE:gI, respectively, and the vaccine, but not IL-4. Immunization with gH:gL and the toxin showed increased thymidine uptake and production of IL-2 and interferon-gamma (IFN-gamma) of the spleen cells, but not IL-4, depending on the dose of gH:gL used for immunization and restimulation in vitro. Purified gE:gI and gB have been reported to be the strongest stimulators of cellular immunity to varicella upon subcutaneous injection and are useful as a subunit vaccine. All the glycoproteins tested are excellent stimulators of cellular immunity to the virus and itself on nasal co-immunization with the toxin.
authors.affiliation: Department of Microbiology, School of Medicine, Fujita Health University, Toyoake, Aichi, Japan. ttsuji@fujita-hu.ac.jp
authors.forename: Takao
authors.initials: T
authors.lastname: Tsuji
authors.forename: Kimiyasu
authors.initials: K
authors.lastname: Shiraki
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sato
authors.forename: Keiko
authors.initials: K
authors.lastname: Sasaki
authors.forename: Michiko
authors.initials: M
authors.lastname: Arita
authors.forename: Michio
authors.initials: M
authors.lastname: Kato
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Sadayuki
authors.initials: S
authors.lastname: Ochi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Ichinose
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokochi
authors.forename: Yoshizo
authors.initials: Y
authors.lastname: Asano
mesh_terms: D000276:Adjuvants, Immunologic; D000281:Administration, Intranasal; D000818:Animals; D001427:Bacterial Toxins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D019433:Chickenpox Vaccine / Q000008:administration & dosage / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D004768:Enterotoxins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D014645:Herpesvirus 3, Human / Q000276:immunology*; D007111:Immunity, Cellular; D007114:Immunization; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D014764:Viral Proteins / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D001427:Bacterial Toxins; D019433:Chickenpox Vaccine; D016207:Cytokines; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D008562:Membrane Glycoproteins; D014764:Viral Proteins; C094648:glycoprotein gL, varicella-zoster virus; C040496:gp 118 protein, Herpesvirus 3, Human; C027538:heat-labile enterotoxin, E coli