[Effect of electroacupuncture on mRNA expression of NGF and IGF-1 in injured nerve].
OBJECTIVE
To investigate the effect of electroacupuncture on mRNA expression of NGF and IGF-1 in injured nerve.


METHODS
Sciatic nerve injury model was established by transection of right side sciatic nerve in 90 male SD rats, which were randomly divided into two groups. The experimental group was treated with electroacupuncture, no treatment in the control group. The distal part of the injured nerve was harvested after 1, 2, 4, 6 and 10 weeks of operation and stored in the liquid nitrogen. The total RNA was extracted by the TRIzol reagent. Reverse transcriptase-polymerase chain reaction(RT-PCR) was used to detected the mRNA expression of NGF and IGF-1.


RESULTS
The mRNA expression of NGF in the experimental group was increased quickly from the second week, and reached to highest level in the fourth week. It was much higher than that of the control group (P < 0.05). Then it began to decline in following time and approximately reached to the level of the first week after 10 weeks of operation. The mRNA expression of IGF-1 in the experimental group was remarkably increased in the second and fourth week, and which was much higher than that of the control group respectively(P < 0.05). Although the mRNA expression of IGF-1 after 10 weeks of operation in the experimental group was higher than that of the control group, but there was no significant difference between the two groups(P > 0.05). There was linear correlation in the fourth week between mRNA expression of NGF and IGF-1 in the experimental group.


CONCLUSIONS
The mRNA expression of NGF and IGF-1 can be elevated in injured nerve at early stage interfered with electroacupuncture.
Chen
X D
XD
Department of Orthopedic Surgery, Xinhua Hospital, Shanghai Second Medical University, Shanghai, P. R. China 200092.
Gu
Y D
YD
Yang
Y
Y
D000818:Animals; D015671:Electroacupuncture; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D020932:Nerve Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D009416:Nerve Regeneration; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries* / Q000378:metabolism
D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D020932:Nerve Growth Factor