[New tactics of human red blood cells stored at 4 degrees C-protective effect of antioxidant solution on red blood cells damage].
Aliquots of venous blood from healthy donor were collected in plastic blood storage bags with ACD, GMA or antioxidant solution (superoxide dismutase, SOD), respectively, and stored at 4 degrees C. After storage for varying periods, the parameters of the blood were detected in the blood samples. Results showed that the parameters of the blood stored at 4 degrees C for 75 days in SOD group were following: the recovery of RBC-Hb was 87.2%, plasma-Hb (mg/L) was 193.2, P50 (mmHg) was 34.0 (normal value was 33.1); deformability (DImax) was 0.2413 (74.3% of normal value). There was no evident hemolysis, color change, air bubble and clots. It was concluded that human RBC stored at 4 degrees C for 75 days with SOD solution, recovery of levels of RBC-Hb and plasma-Hb were accorded with the requirements of "Basic Demands of Blood Station" in China.
Ma
En-Pu
EP
Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China. enpuma@yahoo.com
Liu
Xiu-Zhen
XZ
Han
Ying
Y
Liu
Ming-Dong
MD
Ren
Su-Ping
SP
Liu
An
A
Jin
Peng
P
Bu
Feng-Rong
FR
Wu
Zu-Ze
ZZ
D000975:Antioxidants / Q000494:pharmacology*; D001793:Blood Preservation / Q000379:methods*; D003080:Cold Temperature; D004907:Erythrocyte Deformability / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D006454:Hemoglobins / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D013482:Superoxide Dismutase / Q000494:pharmacology; D013997:Time Factors
D000975:Antioxidants; D016166:Free Radical Scavengers; D006454:Hemoglobins; D013482:Superoxide Dismutase