title: Strategies and outcomes of the first 100 cycles of preimplantation genetic diagnosis at the Guy's and St. Thomas' Center.
abstract: OBJECTIVE
  To establish strategies for the implementation of a successful preimplantation genetic diagnosis (PGD) service.
  
  
  METHODS
  Retrospective review of data from a single center.
  
  
  METHODS
  A United Kingdom National Health Service hospital.
  
  
  METHODS
  Patients (60 couples) were referred for PGD from UK genetic centers.
  
  
  METHODS
  We followed the protocol of ovarian stimulation, oocyte retrieval, fertilization, single cell biopsy on day 3, and embryo transfer on day 4. Pregnancies unaffected by the familial genetic condition.
  
  
  RESULTS
  A total of 60 couples was treated for 20 different conditions. Early cycles using nonsequential embryo culture media were less successful (13% pregnancy rate/embryo transfer) than later cycles using sequential media (33.5%). Ninety-four percent of embryos (n = 473) had a single cell removed at biopsy. The overall pregnancy rate was 24% per cycle started, 29% per egg collection, 38% per transfer, and 40% per couple treated. In one cycle, an affected pregnancy followed PGD for spinal muscular atrophy (SMA).
  
  
  CONCLUSIONS
  The use of sequential media and single cell biopsy results in a successful PGD program with encouraging pregnancy rates.
authors.affiliation: Center for Preimplantation Genetic Diagnosis, Guy's and St. Thomas' NHS Trust, Guy's Hospital, London Bridge, London, United Kingdom. susan.pickering@kcl.ac.uk
authors.forename: Susan
authors.initials: S
authors.lastname: Pickering
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Polidoropoulos
authors.forename: Jenny
authors.initials: J
authors.lastname: Caller
authors.forename: Paul
authors.initials: P
authors.lastname: Scriven
authors.forename: Caroline Mackie
authors.initials: CM
authors.lastname: Ogilvie
authors.forename: Peter
authors.initials: P
authors.lastname: Braude
mesh_terms: D002869:Chromosome Aberrations; D015925:Cryopreservation; D046508:Culture Techniques; D004624:Embryo Transfer; D004622:Embryo, Mammalian; D005260:Female; D005307:Fertilization in Vitro; D030342:Genetic Diseases, Inborn / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D008297:Male; D010062:Ovulation Induction; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011256:Pregnancy Outcome; D019836:Preimplantation Diagnosis; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D016896:Treatment Outcome; D006113:United Kingdom