[Effect of UV-radiation on the level of ascorbic acid, SH-groups, and activity of glutathione reductase in the eye lens].
The effect of UV radiation in vitro on the level of ascorbate, SH-groups and glutathione reductase activity in the soluble fraction of bovine eye lens was studied. UV-Irradiation increased NADPH-oxidoreductase activity, the level of ascorbate oxidation and decreased the content of SH-groups and activity of glutathione reductase. Significant activation of the NADPH-oxidoreductase activity in the presence of ascorbate and Cu2+ was observed after UV-irradiation. It is suggested that ascorbate may play an important role in the UV-induced lens pathology.
Byshneva
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LN
Byelorussion State University, Biology Faculty, Department of Biochemistry, 220080, Minsk, F. Scorina Pr, 4.
Senchuk
V V
VV
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D002417:Cattle; D005980:Glutathione Reductase / Q000378:metabolism*; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D018384:Oxidative Stress / Q000528:radiation effects; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D013438:Sulfhydryl Compounds; D005980:Glutathione Reductase; D001205:Ascorbic Acid