DNA cytometry in pleomorphic adenomas with cytologic atypia.
OBJECTIVE
To investigate the nuclear DNA content of pleomorphic adenomas with cytologic atypia.


METHODS
Analysis was performed on new, fuchsin-stained samples of 10 selected cases of pleomorphic adenoma with cytologic atypia. Morphometric analysis was done by a computer-assisted image cytometry system and consisted of the determination of DNA indices, Auer DNA histogram types, and 3c and 5c exceeding rates.


RESULTS
Eight cases were diploid and two cases aneuploid according to the DNA index. The Auer histogram was type I in five cases and type III in the others. In the two aneuploid cases the 3c exceeding rate was > 10% and the 5c exceeding rate > 1%.


CONCLUSIONS
Atypical cells in pleomorphic adenomas with cytologic atypia carry abnormal amounts of DNA. Image cytometry can make detecting very low numbers of aneuploid cells easier due to its higher resolution as compared to that of flow cytometry.
Safali
Mükerrem
M
Department of Pathology, Gülhane Military Medical Academy, Medical Faculty, Ankara, Turkey. msafali@superonline.com
Celasun
Bülent
B
Günhan
Omer
O
D008949:Adenoma, Pleomorphic / Q000473:pathology / Q000648:ultrastructure*; D000328:Adult; D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006801:Humans; D019044:Image Cytometry; D008297:Male; D008875:Middle Aged; D011003:Ploidies; D012468:Salivary Gland Neoplasms / Q000473:pathology / Q000648:ultrastructure*
D004273:DNA, Neoplasm