[Human umbilical cord blood hematopoietic stem/progenitor cells can grow up in the mouse liver].
The biological characterization, differentiation and regeneration of hepatic stem/progenitor cells are the one of very active and interested fields. In this report, intravenous injection of human umbilical cord blood (HUCB) cells into the BALB/c-nu and SCID mice, an animal model for transplantation and liver injury, was reported. Using of flow cytometry and tissue typing (HLA), it was found that the HUCB cells were survived in mouse liver for 9 weeks. After separation from perfused liver, HUCB cells were detected by hematopoietic colonies (CFU-GEM M) in hepatocyte culture. It was concluded that the transplanted HUCB hematopoietic stem/progenitor cells can be survived in the liver over a long period of time.
Zhou
Sheng-Li
SL
Shandong Cord Blood Stem Cell Center, Jinan 250002, China. shengliz@public3.bta.net.cn
Dong
Zheng-Jun
ZJ
Song
Jian-Qiu
JQ
Luan
Zuo
Z
Zhao
Chun-Hua
CH
Yan
Wen-Ying
WY
Guo
Shuang-Feng
SF
Qu
Wei-Hong
WH
Song
Dao-Gang
DG
D000818:Animals; D002455:Cell Division; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn; D008099:Liver / Q000166:cytology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID
D006684:HLA-DR Antigens