Temporary inactivation of the bed nucleus of the stria terminalis but not of the amygdala blocks freezing induced by trimethylthiazoline, a component of fox feces.
Presentation of trimethylthiazoline (TMT, a component of fox feces) to laboratory rats elicits freezing, a prominent behavioral sign of anxiety or fear. The present study investigated the neural basis of this unlearned response. Muscimol, a GABA(A) receptor agonist, was injected (4.4 nmol/0.5 microl) into the bed nucleus of the stria terminalis (BNST) as well as into the amygdala, two brain areas known to be involved in anxiety and fear. Temporary inactivation of the BNST but not of the amygdala significantly blocked TMT-induced freezing. This effect was not caused by an enhancement of motor activity after BNST inactivation. In addition, these results confirm previous studies showing that freezing is possible despite amygdala inactivation. These results, and other findings in the literature, suggest that the BNST is critically involved in unlearned fear, whereas the amygdala is more involved in the acquisition and expression of learned fear.
Fendt
Markus
M
Tierphysiologie, Universität Tübingen, D-72076 Tübingen, Germany. markus.fendt@uni-tuebingen.de
Endres
Thomas
T
Apfelbach
Raimund
R
D000679:Amygdala / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D001007:Anxiety; D001522:Behavior, Animal; D003213:Conditioning, Psychological; D005239:Fear; D005243:Feces / Q000737:chemistry; D005589:Foxes; D018755:GABA Agonists / Q000633:toxicity; D007267:Injections; D007700:Kinetics; D009043:Motor Activity / Q000187:drug effects; D009118:Muscimol / Q000008:administration & dosage / Q000633:toxicity; D009812:Odorants; D051381:Rats; D017207:Rats, Sprague-Dawley; D012686:Septal Nuclei / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D013844:Thiazoles / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology*
C451263:2,4,5 trimethylthiazoline; D018755:GABA Agonists; D013844:Thiazoles; D009118:Muscimol