Development of a dual recombinant vaccine to protect small ruminants against peste-des-petits-ruminants virus and capripoxvirus infections.
A recombinant capripoxvirus vaccine containing a cDNA of the peste-des-petits-ruminants virus (PPRV) fusion protein gene was constructed. A quick and efficient method was used to select a highly purified recombinant virus clone. A trial showed that a dose of this recombinant as low as 0.1 PFU protected goats against challenge with a virulent PPRV strain.
Berhe
G
G
Cirad, Programme Santé Animale, Campus International de Baillarguet, 34398 Montpellier Cedex 05, France.
Minet
C
C
Le Goff
C
C
Barrett
T
T
Ngangnou
A
A
Grillet
C
C
Libeau
G
G
Fleming
M
M
Black
D N
DN
Diallo
A
A
D000820:Animal Diseases / Q000517:prevention & control*; D000818:Animals; D001483:Base Sequence; D018151:Capripoxvirus / Q000276:immunology / Q000302:isolation & purification*; D017931:DNA Primers; D018076:DNA, Complementary; D004306:Dose-Response Relationship, Immunologic; D029021:Peste-des-Petits-Ruminants / Q000517:prevention & control* / Q000662:veterinary; D018107:Peste-des-petits-ruminants virus / Q000276:immunology; D011213:Poxviridae Infections / Q000517:prevention & control* / Q000662:veterinary / Q000821:virology; D012418:Ruminants; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D017931:DNA Primers; D018076:DNA, Complementary; D014765:Viral Vaccines