TPU-0037-A, B, C and D, novel lydicamycin congeners with anti-MRSA activity from Streptomyces platensis TP-A0598.
In screening for anti-MRSA antibiotics, novel lydicamycin congeners, TPU-0037-A, B, C and D, were isolated from a culture broth of an actinomycete strain. The producing strain, TP-A0598, was isolated from a seawater sample collected in Toyama Bay, Japan, and identified as Streptomyces platensis based on taxonomic characteristics. TPU-0037-A, B, C and D were purified by HP-20 resin, ODS column chromatographies and preparative HPLC, consecutively, and their structures were determined to be 30-demethyllydicamycin, 14,15-dehydro-8-deoxylydicamycin, 30-demethyl-8-deoxylydicamycin and 8-deoxylydicamycin, respectively, by NMR and MS analyses. The new congeners showed antibiotic activity against gram-positive bacteria including MRSA with the MIC of 1.56 to approximately 12.5 microg/ml.
Furumai
Tamotsu
T
Biotechnology Research Center, Toyama Prefectural University, Kosugi, Toyama 939-0398, Japan. furumai@pu-toyama.ac.jp
Eto
Keitaro
K
Sasaki
Tomomitsu
T
Higuchi
Hiroko
H
Onaka
Hirayasu
H
Saito
Noriko
N
Fujita
Takeshi
T
Naoki
Hideo
H
Igarashi
Yasuhiro
Y
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005233:Fatty Alcohols / Q000494:pharmacology; D005285:Fermentation; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D011760:Pyrrolidinones / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects*; D013302:Streptomyces; D013329:Structure-Activity Relationship
D000900:Anti-Bacterial Agents; D005233:Fatty Alcohols; D011760:Pyrrolidinones; C068814:lydicamycin