title: Distinct roles of the Src family kinases, SRC-1 and KIN-22, that are negatively regulated by CSK-1 in C. elegans.
abstract: To elucidate the primitive roles of the Src family kinases (SFKs), here we characterized Caenorhabditis elegans orthologues of SFKs (src-1 and kin-22) and their regulator kinase Csk (csk-1). SRC-1 and KIN-22 possess the C-terminal regulatory tyrosines characteristic of SFKs, and their activities are negatively regulated by CSK-1 in a yeast expression system. The src-1 and csk-1 genes are co-expressed in some head neurons, the anchor cell and the tail region, while kin-22 and csk-1 genes are co-expressed in pharyngeal muscles and tail region. Expression of KIN-22 induced morphological defects in the pharynx, whereas expression of SRC-1 did not show any overt phenotype in adult. RNA interference of src-1, but not that of kin-22, caused a developmental arrest in early development. These results suggest that SRC-1 and KIN-22 play distinct roles under the control of CSK-1.
authors.affiliation: Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan. thirose@biken.osaka-u.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Hirose
authors.forename: Makoto
authors.initials: M
authors.lastname: Koga
authors.forename: Yasumi
authors.initials: Y
authors.lastname: Ohshima
authors.forename: Masato
authors.initials: M
authors.lastname: Okada
mesh_terms: D000818:Animals; D000081247:CSK Tyrosine-Protein Kinase; D017173:Caenorhabditis elegans / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D007814:Larva; D008322:Mammals; D009474:Neurons / Q000378:metabolism; D010614:Pharynx / Q000502:physiology; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D051076:Proto-Oncogene Proteins c-fyn; D013623:Tail / Q000378:metabolism; D015003:Yeasts / Q000235:genetics; D019061:src-Family Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; D011518:Proto-Oncogene Proteins; D011494:Protein Kinases; C471235:SRC-1 protein, C elegans; C471236:csk-1 protein, C elegans; C471234:src-2 protein, C elegans; D000081247:CSK Tyrosine-Protein Kinase; D051076:Proto-Oncogene Proteins c-fyn; D019061:src-Family Kinases