Typing of Haemophilus parasuis strains by PCR-RFLP analysis of the tbpA gene.
On the basis of a species-specific PCR assay, a RFLP analysis for typing of Haemophilus parasuis strains was developed and evaluated. Amplification was based on the gene tbpA, encoding a transferrin-binding protein. RFLP analysis of the 1.9-kb tbpA-amplicon using TaqI, AvaI and RsaI endonucleases produced 12 different patterns for the reference strains of the 15 known H. parasuis serovars, and showed a high heterogeneity (33 RFLP groups) for 101 H. parasuis clinical isolates tested. The sensitivity, typeability (100% versus 65% for immunodiffusion), high degree of discrimination (0.93 versus 0.84 for immunodiffusion), simplicity and low cost per test make this PCR-RFLP assay a useful method for typing H. parasuis and, therefore, for studying the epidemiology of outbreaks of Glässer's disease.
de la Puente Redondo
V A
VA
Department of Animal Health, Faculty of Veterinary Medicine, León, Spain.
Navas Méndez
J
J
García del Blanco
N
N
Ladrón Boronat
N
N
Gutiérrez Martín
C B
CB
Rodríguez Ferri
E F
EF
D000818:Animals; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006190:Haemophilus / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D006192:Haemophilus Infections / Q000382:microbiology / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology; D033881:Transferrin-Binding Protein A / Q000737:chemistry / Q000235:genetics*
D004269:DNA, Bacterial; D033881:Transferrin-Binding Protein A