title: An improved method for the purification of the trichothecene deoxynivalenol (vomitoxin) from Fusarium graminearum culture.
abstract: It was hypothesized that a simplified and efficient strategy could be developed for large-scale production and purification of the mycotoxin deoxynivalenol from Fusarium graminearum rice cultures for toxicological studies. F. graminearum R6576 was cultured on rice and extracted with methanol, and the extract was concentrated and subjected to silica gel low-pressure liquid chromatography (LPLC) under a hexane-acetone gradient system. Deoxynivalenol isolation was monitored by thin-layer chromatography, and fractions containing deoxynivalenol were pooled, concentrated, and applied to a second LPLC column under the same conditions. An enriched deoxynivalenol fraction was obtained, which yielded a crystalline material. Repeated crystallization yielded spectroscopically pure deoxynivalenol. The identity of this compound was confirmed by HPLC comparison to an authentic deoxynivalenol standard, FABMS analysis, and comparison of the (1)H and (13)C NMR spectra with published data. This simplified purification scheme eliminated many laborious steps and equipment previously required to obtain gram quantities of crystalline deoxynivalenol for biological testing in animal models.
authors.affiliation: Department of Food Science and Human Nutrition, Institute for Environmental Toxicology, 234 G.M. Trout Building, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Clifford
authors.forename: Qunshan
authors.initials: Q
authors.lastname: Jia
authors.forename: James J
authors.initials: JJ
authors.lastname: Pestka
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods; D002855:Chromatography, Thin Layer; D003460:Crystallization; D003470:Culture Media; D005670:Fusarium / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D012275:Oryza; D014255:Trichothecenes / Q000302:isolation & purification*
chemical_list: D003470:Culture Media; D014255:Trichothecenes; C007262:deoxynivalenol