title: Cloning of mouse Cited4, a member of the CITED family p300/CBP-binding transcriptional coactivators: induced expression in mammary epithelial cells.
abstract: The CITED family proteins bind to CBP/p300 transcriptional integrators through their conserved C-terminal acidic domain and function as coactivators. The 21-kDa mouse Cited4 protein, a novel member of the CITED family, interacted with CBP/p300 as well as isoforms of the TFAP2 transcription factor, coactivating TFAP2-dependent transcription. The cited4 gene consisted of only a single exon located on chromosome 4 at 56.5-56.8 cM flanked by marker genes kcnq4 and scml1. Expression of Cited4 protein was strong and selective in embryonic hematopoietic tissues and endothelial cells. In adult animals, Cited4 showed strong milk cycle-dependent induction in pregnant and lactating mammary epithelial cells. Strong induction of Cited4 expression was also observed in SCp2 mouse mammary epithelial cells during their prolactin-dependent in vitro differentiation. These results implied possible roles for Cited4 in regulation of gene expression during development and differentiation of blood cells, endothelial cells, and mammary epithelial cells.
authors.affiliation: Department of Tumor Biology, Massachusetts General Hospital Cancer Center, Charlestown, Massachusetts 02129, USA.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Yahata
authors.forename: Hiroko
authors.initials: H
authors.lastname: Takedatsu
authors.forename: Sally L
authors.initials: SL
authors.lastname: Dunwoodie
authors.forename: José
authors.initials: J
authors.lastname: Bragança
authors.forename: Tracey
authors.initials: T
authors.lastname: Swingler
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Withington
authors.forename: Jingyung
authors.initials: J
authors.lastname: Hur
authors.forename: Kathryn R
authors.initials: KR
authors.lastname: Coser
authors.forename: Kurt J
authors.initials: KJ
authors.lastname: Isselbacher
authors.forename: Shoumo
authors.initials: S
authors.lastname: Bhattacharya
authors.forename: Toshi
authors.initials: T
authors.lastname: Shioda
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D019556:COS Cells; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D050881:E1A-Associated p300 Protein; D004622:Embryo, Mammalian / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005091:Exons; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D005796:Genes / Q000235:genetics; D017403:In Situ Hybridization; D007438:Introns; D008297:Male; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors; D015533:Transcriptional Activation
chemical_list: C499189:Cited4 protein, mouse; D018076:DNA, Complementary; D009687:Nuclear Proteins; D012333:RNA, Messenger; D015534:Trans-Activators; D014157:Transcription Factors; D004247:DNA; D050881:E1A-Associated p300 Protein; C494361:Ep300 protein, mouse