Strategies for helicase recruitment and loading in bacteria.
DNA replication initiation in prokaryotes and eukaryotes requires the recruitment and loading of a helicase at the replication origin. To subsequently unwind the double-stranded DNA, the helicase must be properly positioned on the separated DNA strands. Several studies have revealed similarities and differences in the mechanisms used by different autonomously replicating DNA elements (replicons) for recruitment and activation of the appropriate helicase. Of particular interest are plasmid replicons that are adapted for replication in diverse bacterial hosts and are therefore intriguingly able to exploit the helicases of distantly related bacterial species. The different molecular mechanisms by which replicons recruit and load helicases are only just beginning to be understood.
Konieczny
Igor
I
Department of Molecular and Cellular Biology, Faculty of Biotechnology, University of Gdansk, 24 Kladki, Poland. igor@biotech.univ.gda.pl
D001426:Bacterial Proteins / Q000378:metabolism*; D004265:DNA Helicases / Q000378:metabolism*; D004261:DNA Replication / Q000502:physiology*; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000378:metabolism; D053488:DnaB Helicases; D029968:Escherichia coli Proteins / Q000378:metabolism; D046911:Macromolecular Substances; D008957:Models, Genetic; D009038:Motion; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D018741:Replication Origin; D012093:Replicon; D013045:Species Specificity
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; C071702:DnaC protein, E coli; D029968:Escherichia coli Proteins; D046911:Macromolecular Substances; D004265:DNA Helicases; D053488:DnaB Helicases