Ion chromatography of phytate in roots and tubers.
The ion chromatographic method for the quantification of phytate (InsP(6)) in foods was adapted for the analysis of roots and tubers. To maximize sensitivity, ultraviolet (UV) detection following postcolumn derivatization was compared with evaporative light-scattering detection (ELSD). Detection limits for phytate were 0.5 and 1 microg for UV and ELSD, respectively. Unidentified peaks eluting close to and after InsP(6) were removed by solid-phase extraction. Phytate was detected in 11 of 15 roots or tubers. The highest phytate levels were 0.169 and 0.133% of the fresh weight of taro (Colocasia esculenta) and yuca (Manihot esculenta), respectively. Potatoes (Solanum tuberosum) contained 0.035-0.073% phytate, whereas no phytate at a detection limit of 0.003% of fresh weight was observed in sweet potatoes (Ipomoea batatas).
Phillippy
Brian Q
BQ
Commodity Utilization and Formosan Subterranean Termite Research Groups, Southern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1100 Robert E. Lee Boulevard, New Orleans, LA70124, USA. bqphil@srrc.ars.usda.gov
Bland
John M
JM
Evens
Terence J
TJ
D002845:Chromatography / Q000379:methods*; D031060:Colocasia / Q000737:chemistry; D002365:Manihot / Q000737:chemistry; D010833:Phytic Acid / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry*; D035281:Plant Tubers / Q000737:chemistry*; D032322:Solanum / Q000737:chemistry; D014675:Vegetables / Q000737:chemistry*
D010833:Phytic Acid