[Activation of random-donor platelet concentrates and platelet prepared from random-donor apheresis collections during storage].
The activation of random-donor platelet concentrates and platelets prepared from random-donor apheresis collections (plateletpheresis) during storage were studied. Percentage of CD62p staining and mean channel fluorescence (MCF) of CD41 of two kinds of platelets during storage on day 0, day 1, day 3 and day 5 were determined by flow cytometry. The results showed that percentages of CD62p staining and MCF of CD41 in plateletpheresis were (18.91 +/- 6.25)%, (19.48 +/- 8.27)%, (22.82 +/- 6.06)%, (56.71 +/- 11.79)% and (8.09 +/- 2.38)%, (8.13 +/- 2.45)%, (8.44 +/- 2.51)%, (19.87 +/- 6.13)%, while the results of platelet concentrates were (30.65 +/- 12.33)%, (31.46 +/- 11.86)%, (32.51 +/- 13.05)%, (63.55 +/- 13.27)% and (10.33 +/- 4.37)%, (11.09 +/- 6.61)%, (13.46 +/- 9.69)%, (24.41 +/- 10.15)%, respectively. The platelet count and pH value were also determined. The platelet number, pH value, percentage of CD62p staining and MCF of CD41 had no significant difference within 3 days of platelet storage. The platelet number and pH value decreased significantly (P < 0.001), while percentages of CD62p staining and MCF of CD41 increased significantly (P < 0.001) on day 5 of storage. It is concluded that the quality of plateletpheresis is better than platelet concentrate.
Lin
Jun-Jie
JJ
Shanghai Blood Center, Shanghai 200051, China. linzhou69@yahoo.com.cn
Fan
Hua-Hua
HH
Ma
Qing
Q
Feng
Ming-Liang
ML
Shen
Xing-Feng
XF
D001782:Blood Donors; D001793:Blood Preservation; D006801:Humans; D019007:P-Selectin / Q000097:blood; D015539:Platelet Activation; D040201:Platelet Membrane Glycoprotein IIb / Q000097:blood; D010983:Plateletpheresis
D019007:P-Selectin; D040201:Platelet Membrane Glycoprotein IIb