[Serum glutathione S-transferase in bromobenzene-induced acute hepato-toxicity in mice].
The activity of serum glutathione S-transferase (GST) was tested in bromobenzene-induced acute hepatotoxicity in mice. The data showed that a lot of small local necrosis in liver biopsy was observed and serum GST activity was increased after 2 h of treatment with bromobenzene (150 mg/kg i.p.) in mice. Serum alanine transaminase (ALT) activity was also gradually enhanced after 8 hours of treatment. The most obvious changes were at 16 hours of bromobenzene treatment, and serum GST activity was firstly restored to normal level after 24 hours of treatment. There were good positive correlations between serum GST, ALT, as well as serum GST and the changes on liver biopsy. Evaluating analysis of the diagnosis test showed that the sensitivity, specificity and accuracy was 89%, 75% and 82% in serum GST test, and 39%, 92% and 64% in serum ALT test respectively. The ROC work curve also demonstrated that serum GST was more sensitive and specific than serum ALT.
Wang
H
H
Department of Pharmacology, Hubei Medical University, Wuhan 430071.
Peng
R
R
Wu
D
D
Li
S
S
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001969:Bromobenzenes / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood* / Q000209:etiology; D005982:Glutathione Transferase / Q000097:blood*; D008297:Male; D051379:Mice
D001969:Bromobenzenes; C032036:bromobenzene; D005982:Glutathione Transferase; D000410:Alanine Transaminase