title: Overproduction of alpha-glucosidase in Aspergillus niger transformed with the cloned gene aglA.
abstract: We have transformed an industrial strain, Aspergillus niger GN-3, with the alpha-glucosidase gene (aglA) from the same strain. Southern hybridization analysis revealed that transformants had multiple copies of the cloned DNA inserted into the host genome. An 11-fold improvement of enzyme production was achieved by transformation with a DNA fragment composed of 1.11 kb of the 5' noncoding region, 3.12 kb of the coding region containing three introns, and 1.2 kb of the 3' noncoding region. It was found that the 3' noncoding region (1.2 kb) was preferable for maximum production of the enzyme in the transformant.
authors.affiliation: Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo113-8657, Japan.
authors.forename: Dong-Gun
authors.initials: DG
authors.lastname: Lee
authors.forename: Ikuko
authors.initials: I
authors.lastname: Nishimura-Masuda
authors.forename: Akira
authors.initials: A
authors.lastname: Nakamura
authors.forename: Makoto
authors.initials: M
authors.lastname: Hidaka
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Masaki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Uozumi