title: Antioxidant systems and lymphocyte proliferation in the horse, sheep and dog.
abstract: To better define the species-specific antioxidant systems and to ascertain the influence of the intracellular redox status on the immune system of different animal species, we determined lymphocyte glutathione peroxidase (GSHPx) activity, plasmatic glutathione levels (GSH) and the effect of H2O2 on the responsiveness of lymphocytes to proliferative stimuli. Among the three species considered, sheep presented the lowest plasmatic GSH and the highest lymphocyte GSHPx activity. On the contrary, dogs showed an inverted pattern (high GSH - low GSHPx). Horses displayed intermediate values for both parameters analysed. The effect of H2O2 on the proliferative capacity of lymphocytes was the same for all three species; the 200 microM dose in particular was strongly inhibiting. Each species, however, showed different rates of inhibition: sheep exhibited the highest sensitivity to the antiproliferative effect of H2O2. Our results confirmed that high H2O2 concentrations (200 microM) are noxious for the cellular functions of all animals; however this effect is mediated by a rigorously species-specific relationship between the intracellular reactive oxygen species (ROS) and the molecular systems involved in cell proliferation.
authors.affiliation: Department of Technology and Biotechnology of Animal Production, University of Perugia, via S. Costanzo 4, 06126 Perugia, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Chiaradia
authors.forename: Alberto
authors.initials: A
authors.lastname: Gaiti
authors.forename: Lucia
authors.initials: L
authors.lastname: Scaringi
authors.forename: Paola
authors.initials: P
authors.lastname: Cornacchione
authors.forename: Pierfrancesco
authors.initials: P
authors.lastname: Marconi
authors.forename: Luca
authors.initials: L
authors.lastname: Avellini
mesh_terms: D000818:Animals; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005260:Female; D005978:Glutathione / Q000097:blood*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006736:Horses; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D012680:Sensitivity and Specificity; D012756:Sheep; D015398:Signal Transduction; D013045:Species Specificity
chemical_list: D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D005978:Glutathione