A highly sensitive quantitative immunochromatography assay for antigen-specific IgE.
We have developed a highly sensitive quantitative enzyme immunochromatography system for antigen-specific IgE, which is clinically important for the diagnosis of allergic diseases. The system uses alkaline phosphatase-labeled anti-IgE antibody (ALP-anti-IgE) and immobilized target antigen, for example cedar pollen antigen, on a membrane. Antigen-specific IgE present in the serum binds the immobilized antigen after complexing with the ALP-anti-IgE. Subsequently, the enzyme substrate migrates to the complex on the antigen line, which is stained blue. The intensity of the staining was analyzed by a quantitative detector, but can also be assessed directly by the naked eye. This system was able to detect 0.2 U/ml of IgE specific for Japanese cedar pollen. The results correlated well (Spearman's rank correlation coefficient, 0.95) to those in AlaSTAT system, which is a reliable enzyme-linked immunosorbent assay (ELISA) method. This antigen-specific IgE assay system is suitable for point-of-care testing.
Ono
Tetsuya
T
Research Division, Iatron Laboratories, Inc., 1460-6 Mitodai, Tako, Katori, Chiba 289-2247, Japan. ono-tetsuya@iatron.co.jp
Kawamura
Masahide
M
Arao
Shinsuke
S
Nariuchi
Hideo
H
D000485:Allergens; D000918:Antibody Specificity; D000941:Antigens; D002845:Chromatography / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D007121:Immunocompetence; D007124:Immunoenzyme Techniques / Q000379:methods* / Q000706:statistics & numerical data; D007073:Immunoglobulin E / Q000032:analysis* / Q000097:blood; D011058:Pollen; D015203:Reproducibility of Results; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology; D012680:Sensitivity and Specificity
D000485:Allergens; D000941:Antigens; D007073:Immunoglobulin E