title: Typing of Haemophilus parasuis strains by PCR-RFLP analysis of the tbpA gene.
abstract: On the basis of a species-specific PCR assay, a RFLP analysis for typing of Haemophilus parasuis strains was developed and evaluated. Amplification was based on the gene tbpA, encoding a transferrin-binding protein. RFLP analysis of the 1.9-kb tbpA-amplicon using TaqI, AvaI and RsaI endonucleases produced 12 different patterns for the reference strains of the 15 known H. parasuis serovars, and showed a high heterogeneity (33 RFLP groups) for 101 H. parasuis clinical isolates tested. The sensitivity, typeability (100% versus 65% for immunodiffusion), high degree of discrimination (0.93 versus 0.84 for immunodiffusion), simplicity and low cost per test make this PCR-RFLP assay a useful method for typing H. parasuis and, therefore, for studying the epidemiology of outbreaks of Glässer's disease.
authors.affiliation: Department of Animal Health, Faculty of Veterinary Medicine, León, Spain.
authors.forename: V A
authors.initials: VA
authors.lastname: de la Puente Redondo
authors.forename: J
authors.initials: J
authors.lastname: Navas Méndez
authors.forename: N
authors.initials: N
authors.lastname: García del Blanco
authors.forename: N
authors.initials: N
authors.lastname: Ladrón Boronat
authors.forename: C B
authors.initials: CB
authors.lastname: Gutiérrez Martín
authors.forename: E F
authors.initials: EF
authors.lastname: Rodríguez Ferri
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006190:Haemophilus / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D006192:Haemophilus Infections / Q000382:microbiology / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology; D033881:Transferrin-Binding Protein A / Q000737:chemistry / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D033881:Transferrin-Binding Protein A