Amino acid residues of Escherichia coli acyl carrier protein involved in heterologous protein interactions.
Acyl carrier protein (ACP) is a small, highly conserved protein with an essential role in a myriad of reactions throughout lipid metabolism in plants and bacteria where it interacts with a remarkable diversity of proteins. The nature of the proper recognition and precise alignment between the protein moieties of ACP and its many interactive proteins is not understood. Residues conserved among ACPs from numerous plants and bacteria were considered as possibly being crucial to ACP's function, including protein-protein interaction, and a method of identifying amino acid residue clusters of high hydrophobicity on ACP's surface was used to estimate residues possibly involved in specific ACP-protein interactions. On the basis of this information, single-site mutation analysis of multiple residues, one at a time, of ACP was used to probe the identities of potential contact residues of ACPSH or acyl-ACP involved in specific interactions with selected enzymes. The roles of particular ACP residues were more precisely defined by site-directed fluorescence analyses of various myristoyl-mutant-ACPs upon specific interaction with the Escherichia coli hemolysin-activating acyltransferase, HlyC. This was done by selectively labeling each mutated site, one at a time, with an environmentally sensitive fluoroprobe and observing its fluorescence behavior in the absence and presence of HlyC. Consequently, a picture of the portion of ACP involved in selected macromolecular interaction has emerged.
Worsham
Lesa M S
LM
Department of Biochemistry and Molecular Biology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614, U SA.
Earls
Laurie
L
Jolly
Carrie
C
Langston
Keisha Gordon
KG
Trent
M Stephen
MS
Ernst-Fonberg
M Lou
ML
D000213:Acyl Carrier Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000215:Acylation; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D000596:Amino Acids / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000817:Anilino Naphthalenesulfonates / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D064429:Fatty Acid Synthases / Q000378:metabolism; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes / Q000378:metabolism; D006460:Hemolysin Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D019814:Myristic Acid / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity / Q000235:genetics; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013439:Sulfhydryl Reagents / Q000378:metabolism
D000213:Acyl Carrier Protein; D000596:Amino Acids; D000817:Anilino Naphthalenesulfonates; D029968:Escherichia coli Proteins; D005456:Fluorescent Dyes; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; D019814:Myristic Acid; C021310:2-(4'-maleimidylanilino)naphthalene-6-sulfonic acid; D064429:Fatty Acid Synthases