Genetic diversity of Plasmodium falciparum field samples from an isolated Colombian village.
Colombian field isolates of Plasmodium falciparum were analyzed for genetic diversity. Fifty-three samples were collected as thick smears from patients living in Panguí, an isolated area with low migration. While the samples were being collected, Panguí was experiencing an epidemic outbreak of malaria. The samples were typified using nested polymerase chain reaction (PCR) amplification of block 2 of the merozoite surface protein 1 (MSP1) gene and nested PCR with mutation-specific primers for position 108 of the dihydrofolate reductase enzyme gene. The results for the circulating population of parasites in Panguí show low diversity--four allelic forms--using MSP1 as a marker, a fact that contrasts with data reported for certain Asian and African zones. A high percentage of mixed infections was observed, as was high complexity of the infection. No differential distributions were found for any allelic type.
Gómez
Diana
D
Laboratory of Biochemistry, Instituto Nacional de Salud, Bogota, Colombia.
Chaparro
Jacqueline
J
Rubiano
Claudia
C
Rojas
María Orfa
MO
Wasserman
Moises
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002648:Child; D002675:Child, Preschool; D003105:Colombia / Q000453:epidemiology; D016054:DNA, Protozoan / Q000032:analysis; D004196:Disease Outbreaks; D005260:Female; D014644:Genetic Variation; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016778:Malaria, Falciparum / Q000453:epidemiology* / Q000469:parasitology; D008297:Male; D020066:Merozoite Surface Protein 1 / Q000235:genetics; D008875:Middle Aged; D010963:Plasmodium falciparum / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012424:Rural Population; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics
D016054:DNA, Protozoan; D020066:Merozoite Surface Protein 1; D013762:Tetrahydrofolate Dehydrogenase