Effect of stress on the ability of a phlA-based quantitative competitive PCR assay to monitor biocontrol strain Pseudomonas fluorescens CHA0.
A quantitative competitive PCR (QC-PCR) assay targeting the phlA gene of Pseudomonas fluorescens CHA0 was developed and tested in vitro. Statistically significant, positive correlations were found between QC-PCR and both CFU and total cell number when studying cells in log or stationary phase. The correlations disappeared when considering stressed cells.
Rezzonico
Fabio
F
Phytopathology Group, Institute of Plant Science, Swiss Federal Institute of Technology (ETH), CH-8092 Zürich, Switzerland.
Moënne-Loccoz
Yvan
Y
Défago
Geneviève
G
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015169:Colony Count, Microbial; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D018869:Heat-Shock Response; D010572:Pest Control, Biological; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011551:Pseudomonas fluorescens / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*
D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D010696:Phloroglucinol