Cutting edge: dissociation between autoimmune response and clinical disease after vaccination with dendritic cells.
Autoimmunity represents a caveat to the use of dendritic cells (DCs) as adjuvant for human vaccines. We derived DCs from normal BALB/c mice or from mice prone to autoimmunity (NZB x NZW) F(1). We allowed DCs to phagocytose apoptotic thymocytes and vaccinated syngeneic animals. All mice developed anti-nuclear and anti-dsDNA Abs. Autoantibodies in normal mice were transient, without clinical or histological features of autoimmunity or tissue involvement. In contrast, autoimmunity was maintained in susceptible mice, which underwent renal failure and precociously died. The data suggest that DC vaccination consistently triggers autoimmune responses. However, clinical autoimmunity develops in susceptible subjects only.
Bondanza
Attilio
A
Cancer Immunotherapy and Gene Therapy Program, Clinical Immunology and Rheumatology Unit, H. San Raffaele Scientific Institute, and Università Vita-Salute San Raffaele, Milan, Italy.
Zimmermann
Valérie S
VS
Dell'Antonio
Giacomo
G
Dal Cin
Elena
E
Capobianco
Annalisa
A
Sabbadini
Maria Grazia
MG
Manfredi
Angelo A
AA
Rovere-Querini
Patrizia
P
D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000276:immunology; D001323:Autoantibodies / Q000096:biosynthesis; D001327:Autoimmune Diseases / Q000235:genetics / Q000276:immunology* / Q000401:mortality / Q000473:pathology; D001854:Bone Marrow Cells / Q000276:immunology; D016026:Bone Marrow Transplantation; D003433:Crosses, Genetic; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology / Q000637:transplantation*; D005260:Female; D005921:Glomerulonephritis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D007678:Kidney Glomerulus / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008814:Mice, Inbred NZB; D010587:Phagocytosis / Q000235:genetics / Q000276:immunology; D013234:Stem Cells / Q000166:cytology / Q000276:immunology; D014611:Vaccination / Q000379:methods
D001323:Autoantibodies