title: LytG of Bacillus subtilis is a novel peptidoglycan hydrolase: the major active glucosaminidase.
abstract: LytG (YubE) of Bacillus subtilis is a novel 32 kDa autolysin produced during vegetative growth under the control of Esigma(A) RNA polymerase. Muropeptide analysis of vegetative cells of B. subtilis revealed LytG to be the major glucosaminidase responsible for peptidoglycan structural determination during vegetative growth. Overexpression and purification of LytG allowed its biochemical characterization. Despite sequence homology suggesting muramidase activity, LytG is a novel glucosaminidase with exoenzyme activity and may form part of a novel family of autolysins. It is involved in cell division, lysis, and motility on swarm plates.
authors.affiliation: Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, United Kingdom.
authors.forename: Gavin J
authors.initials: GJ
authors.lastname: Horsburgh
authors.forename: Abdelmadjid
authors.initials: A
authors.lastname: Atrih
authors.forename: Michael P
authors.initials: MP
authors.lastname: Williamson
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Foster
mesh_terms: D001412:Bacillus subtilis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001426:Bacterial Proteins / Q000737:chemistry* / Q000502:physiology; D001433:Bacteriolysis / Q000502:physiology; D001483:Base Sequence; D002455:Cell Division / Q000502:physiology; D002473:Cell Wall / Q000201:enzymology / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D010450:Endopeptidases / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D006596:Hexosaminidases / Q000737:chemistry* / Q000502:physiology; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009068:Movement / Q000502:physiology; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000032:analysis; D010457:Peptidoglycan / Q000737:chemistry; D011485:Protein Binding
chemical_list: D001426:Bacterial Proteins; D010455:Peptides; D010457:Peptidoglycan; D006596:Hexosaminidases; D010450:Endopeptidases; C019404:gamma-D-glutamyl-meso-diaminopimelate peptidase I; D009238:N-Acetylmuramoyl-L-alanine Amidase