Morphology of calretinin-immunoreactive neurons in the superficial layers of hamster superior colliculus after enucleation: lack of co-localization with GABA.
We recently reported on the distribution and effects of eye enucleation on the immunoreactivity of calretinin in the superficial layers of the hamster superior colliculus (SC). In the present study, we describe the types of labeled cells and compare this labeling to that of GABA, the major inhibitory neurotransmitter in the central nervous system. An almost complete depletion of calretinin-immunoreactive (IR) fibers in the superficial layers of the contralateral SC was found following unilateral enucleation. On the contralateral SC, many calretinin-IR cells were newly appeared. The majority of the newly-appeared cells had small- to medium-sized round, oval, or vertical fusiform cell bodies. Two-color immunofluorescence revealed that none of these newly-appeared cells were labeled with an antibody to GABA. The present results show that the calretinin-IR cells are unique in the superficial hamster SC when compared to most of the other brain areas, where many calretinin-IR cells are GABAergic interneurons.
Kang
Youn-Sil
YS
Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu 702-701, Korea.
Kong
Jee-Hyun
JH
Park
Won-Mee
WM
Kwon
Oh-Ju
OJ
Lee
Ji-Eun
JE
Kim
Si-Yeol
SY
Jeon
Chang-Jin
CJ
D000818:Animals; D064032:Calbindin 2; D006224:Cricetinae; D015353:Eye Enucleation; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes; D009412:Nerve Fibers / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism; D010477:Perfusion; D012165:Retinal Ganglion Cells / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013477:Superior Colliculi / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D064032:Calbindin 2; D005456:Fluorescent Dyes; D009419:Nerve Tissue Proteins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G; D005680:gamma-Aminobutyric Acid