Determination of 6-hydroxychlorzoxazone and chlorzoxazone in porcine microsome samples.
A simple, accurate and sensitive HPLC method for the in vitro determination of 6-hydroxychlorzoxazone and chlorzoxazone in porcine microsome samples is described. Chromatography was performed on a YMC-Pack ODS-AQ column using a mobile phase of 0.05% phosphoric acid pH 3-methanol (60:40, v/v). UV detection was carried out at 287 nm. The detector response was linear over the concentration range 25-2000 ng/ml. This assay produced quick, accurate, and repeatable results.
Cox
Sherry K
SK
Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, 37901, Knoxville, TN, USA. scox6@utk.edu
Hamner
Tina
T
Bartges
Joe
J
D000818:Animals; D002753:Chlorzoxazone / Q000031:analogs & derivatives* / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D008861:Microsomes / Q000737:chemistry*; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
C039289:6-hydroxychlorzoxazone; D002753:Chlorzoxazone