Proline 78 is crucial for human immunodeficiency virus type 1 Nef to down-regulate class I human leukocyte antigen.
Human immunodeficiency virus type 1 Nef down-regulates human leukocyte antigen class I (HLA-I) in T lymphocytes, and the down-regulation involves the Nef proline-rich domain (PRD) containing four prolines at positions 69, 72, 75, and 78. We used a Sendai virus vector with nef and examined regulation by Nef of HLA-I and CD4 in suspension cultures of cells such as T lymphocytes. Analyses of a series of PRD substitution mutants indicated that, because the substitution of Pro78 with Ala abolished down-regulation of HLA-I but not of CD4, Pro78 is important for HLA-I down-regulation in T lymphocytes.
Yamada
Takeshi
T
Division of Infectious Diseases, Advanced Clinical Research Center, The University of Tokyo, Japan.
Kaji
Naotoshi
N
Odawara
Takashi
T
Chiba
Joe
J
Iwamoto
Aikichi
A
Kitamura
Yoshihiro
Y
D002460:Cell Line; D015536:Down-Regulation / Q000502:physiology*; D015693:Gene Products, nef / Q000737:chemistry / Q000502:physiology*; D015497:HIV-1 / Q000502:physiology*; D015395:Histocompatibility Antigens Class I / Q000502:physiology*; D006801:Humans; D011392:Proline / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology; D054311:nef Gene Products, Human Immunodeficiency Virus
D015693:Gene Products, nef; D015395:Histocompatibility Antigens Class I; D054311:nef Gene Products, Human Immunodeficiency Virus; D011392:Proline