title: The effect of nickel sulphate, potassium dichromate, cobalt nitrate and cadmium sulphate on the proteins of cellular contacts and actin skeleton of cultivated human keratinocytes.
abstract: The authors tested the effects of known allergens, namely nickel sulphate, potassium dichromate, cobalt nitrate and cytotoxic cadmium sulphate on the proteins of cellular contacts (vinculin, talin, E-cadherin, desmoplaktin) and actin cytoskeleton (actin filaments) of cultivated human keratinocytes. The localisation of proteins of cellular contacts was detected by means of direct immunofluorescence. The authors have detected a decrease in, and destruction of cellular contact proteins and actin cytoskeleton after testing the effect of all allergens, while the most significant changes were detected in E-cadherin, vunkulin and actin filaments. Desmoplaktin and talin were less damaged. Potassium dichromate caused damage already in concentration of 1 microg/ml. A similar effect of the other two tested haptanes was brought about in concentration being 100-fold higher.
  
  
  CONCLUSIONS
  the gained results indicate that the investigation of cellular contact proteins and actin cytoskeleton of cultivated human keratinocytes can possibly become a part of the testing of allergy-triggering potential of chemical substances. (Tab. 1, Fig. 4, Ref. 18.)
authors.affiliation: 1st Department of Dermatovenerology, Faculty of Medicine, Comenius University Bratislava, Slovakia. bll@fmed.uniba.sk
authors.forename: A
authors.initials: A
authors.lastname: Lozsekova
authors.forename: H W
authors.initials: HW
authors.lastname: Kaiser
authors.forename: T
authors.initials: T
authors.lastname: Danilla
authors.forename: J
authors.initials: J
authors.lastname: Buchvald
authors.forename: J
authors.initials: J
authors.lastname: Simko
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000485:Allergens / Q000494:pharmacology*; D019187:Cadmium Compounds / Q000494:pharmacology; D002478:Cells, Cultured; D003035:Cobalt / Q000494:pharmacology; D003598:Cytoskeletal Proteins / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007365:Intercellular Junctions / Q000187:drug effects* / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D008670:Metals / Q000494:pharmacology*; D009532:Nickel / Q000494:pharmacology; D011192:Potassium Dichromate / Q000494:pharmacology; D013431:Sulfates / Q000494:pharmacology
chemical_list: D000485:Allergens; D019187:Cadmium Compounds; D003598:Cytoskeletal Proteins; D008670:Metals; D013431:Sulfates; D003035:Cobalt; C029938:nickel sulfate; C025913:cobaltous nitrate; D009532:Nickel; C037123:cadmium sulfate; D011192:Potassium Dichromate