[Determination of CD34(+) cells in mobilized peripheral blood by flow cytometry].
To explore a simple and effective method to determinate the volume of CD34(+) cells in the peripheral blood of donors received drug mobilization for stem cell transplantation by using flow cytometry, the mobilized peripheral blood from donors and 100 micro l fresh whole blood were labeled with monoclonal antibodies Anti-CD34-PE and Anti-CD45-FITC, after lying the red blood cells, and assessed with flow cytometer FL2 (log) vs SSC (log) and FL1 (log) vs SSC (log) were mainly used for analysis windows. The results showed that a level of CD34(+) cells in whole nucleated cells as low as 0.05% - 0.1% can be detected effectively using this method when 10(5) nucleated cells were counted. At day 5 or day 6, the level of CD34(+) cells in most samples of patients reached a peak volume, some of samples and the levels were more than one percent in. It was concluded that CD34(+) cells can be effectively determined by using this method. According to the relative rate of CD34(+) cells, the time to harvest the stem cells in blood can be determined.
Song
Wen-Gang
WG
Department of Immunology, Taishan Medical College, Taian 27100, China.
Zhang
Ming-Hui
MH
Zhang
Wei-Ping
WP
Qu
Xun
X
Song
Xian-Min
XM
Wang
Jian-Min
JM
Cao
Xue-Tao
XT
D018952:Antigens, CD34 / Q000097:blood*; D001782:Blood Donors; D005434:Flow Cytometry / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans
D018952:Antigens, CD34