The physiological response of diploid and triploid brook trout to exhaustive exercise.
Using triploidy as an experimental model, we examined whether cell size limits the post-exercise recovery process in fish. Because triploids generally possess larger cells, which could affect many physiological and biochemical processes, we hypothesized that triploids would take longer to recover from exhaustive exercise compared to diploids. To test this, we measured plasma lactate, glucose and osmolality, and white muscle energy stores (glycogen, phosphocreatine and ATP) and lactate before and immediately following exhaustive exercise and during recovery at 2 and 4 h post-exercise. In addition, oxygen consumption and ammonia excretion rates were determined before and after exhaustive exercise. Overall, diploid and triploid brook trout showed similar metabolic responses exercise, but plasma osmolality, white muscle lactate, white muscle ATP and post-exercise oxygen consumption rates recovered earlier in triploids compared to diploids. The results of this study suggest that the characteristic larger cell size of triploidy does not limit the physiological response to, or recovery from, exhaustive exercise.
Hyndman
C A
CA
Department of Biology and Centre for Coastal Studies and Aquaculture, University of New Brunswick, PO Box 5050, Saint John, N B E2L 4L5, Canada.
Kieffer
J D
JD
Benfey
T J
TJ
D000255:Adenosine Triphosphate / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D048429:Cell Size / Q000502:physiology; D004171:Diploidy; D004734:Energy Metabolism / Q000502:physiology; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism; D006019:Glycolysis / Q000502:physiology; D019344:Lactic Acid / Q000097:blood; D018482:Muscle, Skeletal / Q000378:metabolism; D009994:Osmolar Concentration; D010101:Oxygen Consumption / Q000502:physiology; D010807:Physical Endurance / Q000502:physiology*; D011123:Polyploidy; D014337:Trout / Q000235:genetics / Q000378:metabolism*; D014867:Water / Q000378:metabolism
D001786:Blood Glucose; D014867:Water; D019344:Lactic Acid; D000641:Ammonia; D000255:Adenosine Triphosphate