Pap1-dependent regulation of the GSTII gene from the fission yeast.
The genomic DNA encoding a second glutathione S-transferase (GSTII) was previously isolated from the fission yeast Schizosaccharomyces pombe. Its expression was shown to be induced by menadione, mercuric chloride, o-dinitrobenzene, and NO-generating S-nitroso-N-acetylpenicillamine using the GSTII-lacZ fusion harboring the 910 bp upstream region from the translational initiation point. In this study, the additional fusion plasmids pGST50-590 and pGST50-6R-590 were constructed to carry the 590 bp upstream region in the vectors YEp357 and YEp367R, respectively. The synthesis of beta-galactosidase from the fusion plasmid pGST50-590 was about 3-fold higher than that from the fusion plasmid pGST50-F, indicating the presence of negatively activating sequence in the -910 to approximately -590 region. It was also enhanced by the same agents, which induced the synthesis of beta-galactosidase from the fusion plasmid pGST50-F. The synthesis of beta-galactosidase from both fusion plasmids pGST50-F and pGST50-590 was enhanced by the overexpressed Pap1 protein. The synthesis of beta-galactosidase from the two YEp367R derivatives pGST50-6R-F and pGST50-6R-590 was greatly decreased in the Pap1-negative strain TP108-3C. These results propose the Pap1-dependent regulation of the GSTII gene from the fission yeast.
Lim
Chang-Jin
CJ
Division of Life Sciences, Kangwon National University, Chuncheon 200-701, Korea. cjlim@kangwon.ac.kr
Cho
Young-Wook
YW
Sa
Jae-Hoon
JH
Lim
Hye Won
HW
Kim
Hong-Gyum
HG
Kim
Su-Jung
SJ
Park
Eun-Hee
EH
D050976:Basic-Leucine Zipper Transcription Factors; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000502:physiology*; D004136:Dinitrobenzenes / Q000494:pharmacology; D004202:Disinfectants / Q000494:pharmacology; D015536:Down-Regulation; D005656:Fungal Proteins / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D008627:Mercuric Chloride / Q000494:pharmacology; D009154:Mutation / Q000235:genetics; D000073718:Pancreatitis-Associated Proteins; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012568:Schizosaccharomyces / Q000201:enzymology*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation; D001616:beta-Galactosidase / Q000378:metabolism*
D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D004136:Dinitrobenzenes; D004202:Disinfectants; D005656:Fungal Proteins; D000073718:Pancreatitis-Associated Proteins; C105096:Pap1 protein, S pombe; C000612141:REG3A protein, human; D029702:Schizosaccharomyces pombe Proteins; C040234:1,2-dinitrobenzene; D008627:Mercuric Chloride; D005982:Glutathione Transferase; D001616:beta-Galactosidase; C041116:leukotriene-C4 synthase