Relationship between the changes in cellular volume of fish spermatozoa and their cryoresistance.
We have investigated the hypothesis that the spermatozoa of marine fish are more resistant than freshwater species to the dynamic changes in osmotic pressure that occur during the process of cryopreservation. We show that while the spermatozoa of marine fish can be successfully activated across a wide range of osmotic pressures (0-2000 mOsmol/l), those of the freshwater species only survive activation within a more restricted range (0-300 mOsm/l). After freeze-thawing, up to 30 percent of motile cells were found in silver carp samples, while up to 90 percent of motile cells were observed in samples from the haarder (Mugil soiuy B). Haarder spermatozoa showed no change of cell volume after dilution in activating or cryoprotective media, while the silver carp spermatozoa responded by swelling and eventual cell disruption. We propose that the differences in cryoresistance of silver carp (Hypophthalmichthys molitrix V.) and haarder spermatozoa may be determined by the ability to preserve cellular volume under non-isotonic conditions.
Dzuba
B B
BB
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, 23, Pereyaslavskaya str, 61015, Kharkov, Ukraine. cryo@online.kharkov.ua
Kopeika
E F
EF
D000818:Animals; D002347:Carps; D002470:Cell Survival; D015925:Cryopreservation; D005399:Fishes; D005618:Fresh Water; D008297:Male; D009997:Osmotic Pressure; D012623:Seawater; D012662:Semen Preservation; D012965:Sodium Chloride; D013081:Sperm Motility
D012965:Sodium Chloride