Expression of protein phosphatase 1 during the asexual development of Neurospora crassa.
We cloned and sequenced the cDNA and the gene encoding the catalytic subunit of protein phosphatase 1 from the filamentous fungus Neurospora crassa. The gene, designated ppp-1 (phosphoprotein phosphatase 1), was mapped by restriction fragment length polymorphism to linkage group III, in the vicinity of con-7 and trp-1. The expression of the gene was monitored by reverse transcriptase and polymerase chain reactions, by Western blotting, and by protein phosphatase activity assays in synchronized cultures. Transcripts of ppp-1 were detected in the dormant conidia. The abundance of ppp-1 mRNA, Ppp-1 protein, and the activity of protein phosphatase 1 increased during germination and subsequent hyphal elongation as well as during the early stages of aerial mycelium formation.
Zeke
Tamás
T
Department of Medical Chemistry, Medical and Health Sciences Center, University of Debrecen, H-4026 Debrecen, Hungary.
Kókai
Endre
E
Szöor
Balázs
B
Yatzkan
Einat
E
Yarden
Oded
O
Szirák
Krisztina
K
Fehér
Zsigmond
Z
Bagossi
Péter
P
Gergely
Pál
P
Dombrádi
Viktor
V
D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D015723:Gene Library; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000201:enzymology*; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis* / Q000737:chemistry*; D010766:Phosphorylation; D012150:Polymorphism, Restriction Fragment Length; D014176:Protein Biosynthesis; D054645:Protein Phosphatase 1; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013997:Time Factors
D018076:DNA, Complementary; D012333:RNA, Messenger; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1