Identification of a candidate oncogene SEI-1 within a minimal amplified region at 19q13.1 in ovarian cancer cell lines.
High-level amplification of DNA sequence at 19q13.1 is one of the frequent genetic alterations in ovarian cancer. In an attempt to verify the minimal amplified region (MAR) at 19q13.1 and to identify the target oncogenes, 49 probes within a region from D19S425 to D19S907 ( approximately 19.5 cM) were used to survey the amplification status in four ovarian cancer cell lines that have been confirmed as containing amplification at 19q13.1. Two separated overlapping MARs, MAR1 (approximately 200 kb) and MAR2 (approximately 1.1 Mb), were identified at 19q13.1. Two candidate oncogenes, AKT2 and SEI-1, were identified in MAR2. Amplification and overexpression of these two genes in four ovarian cancer cell lines were confirmed by Southern and Northern blot analyses. The proliferation-related function of AKT2 and SEI-1 suggests that both genes are likely to be biological targets of an amplification event at 19q13.1 in ovarian cancer and to play important roles in ovarian tumorigenesis.
Tang
Terence C-M
TC
Department of Clinical Oncology, The University of Hong Kong, Hong Kong, China.
Sham
Jonathan S T
JS
Xie
Dan
D
Fang
Yan
Y
Huo
Ke-Ke
KK
Wu
Qiu-Liang
QL
Guan
Xin-Yuan
XY
D015139:Blotting, Southern; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D015870:Gene Expression; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009687:Nuclear Proteins; D009857:Oncogenes / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics*; D020161:Physical Chromosome Mapping / Q000379:methods; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins / Q000235:genetics; D051057:Proto-Oncogene Proteins c-akt; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors; D014407:Tumor Cells, Cultured
D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; C402464:SERTAD1 protein, human; D015534:Trans-Activators; D014157:Transcription Factors; C494921:AKT2 protein, human; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt