title: Circularly polarised MF (500 micro T 50 Hz) does not acutely suppress melatonin secretion from cultured Wistar rat pineal glands.
abstract: Magnetic fields (MF, 50 Hz) have been proposed to affect melatonin production in mammals; however, there is very little data about the mechanism by which this possible interaction may occur. Here we describe results from the first study in which circularly polarised 50 Hz MF have been administered to isolated pineals in highly controlled conditions. Melatonin release from isolated Wistar rat pineal glands, dissected 2 h after light onset ZT 2, was measured in a flow through culture system, during and after exposure to a 4 h MF similar in nature and magnitude to that produced in extremely close proximity to a high voltage power line (500 micro T 50 Hz circularly polarised). Melatonin release from isolated pineals was comparable to that observed in previous studies, plateauing to approximately 100 pg/ml/30 min. No significant alterations in pineal melatonin release were caused by exposure to the MF when compared to sham exposure (< 1 micro T). These results suggest that if the circadian system is acutely responsive to MF exposure of this nature, an intact circadian axis may be necessary in order to observe an effect on the production on melatonin from the pineal gland
authors.affiliation: Centre for Chronobiology, School of Biomedical and Life Sciences, University of Surrey, Guildford, Surrey, United Kingdom.
authors.forename: H M
authors.initials: HM
authors.lastname: Tripp
authors.forename: G R
authors.initials: GR
authors.lastname: Warman
authors.forename: J
authors.initials: J
authors.lastname: Arendt
mesh_terms: D000818:Animals; D046508:Culture Techniques; D004574:Electromagnetic Fields; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D008297:Male; D008550:Melatonin / Q000032:analysis / Q000378:metabolism*; D010870:Pineal Gland / Q000378:metabolism* / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012680:Sensitivity and Specificity
chemical_list: D008550:Melatonin