[Dendritic cells cultured with human umbilical cord serum instead of fetal calf serum].
To investigate whether the dendritic cells (DC) could grow up in cultural system with umbilical cord serum (UCS), the UCS was used in the culture instead of fetal calf serum. The phenotype of dendritic cells was detected by flow cytometry and the antigen presenting ability of DC in allo-MLR was measured by MTT assay. The results showed that DC grown in UCS (UCS-DC) had higher expression rate of CD86, CD83 and HLA-DR than that in grown in FCS (FCS-DC). (P < 0.05), and their expression of CD1a was lower than that of FCS-DC. The ability to induce T cell proliferation had no difference between UCS-DC and FCS-DC. It is suggested that dendritic cells with more mature phenotype had been produced in the medium containing UCS than those in the medium containing FCS, and UCS-DC possessed potent ability to stimulate proliferation of allogeneic T cells.
Wang
Li-Ru
LR
Department of Hematology, Fu Xing Hospital, Capital University of Medical Sciences, Beijing 100038, China. Wangliru@sohu.com
Shi
Bin
B
Cui
Jian-Ying
JY
Han
Shu-Xia
SX
Wang
Shen-Wu
SW
Chen
Jin-Li
JL
D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D051940:B7-2 Antigen; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D005312:Fetal Blood / Q000502:physiology*; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology; D000097802:CD83 Antigen
D015703:Antigens, CD; D051940:B7-2 Antigen; D003470:Culture Media; D006684:HLA-DR Antigens; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen; C497964:CD86 protein, human