Diversity of the human erythrocyte membrane sialic acids in relation with blood groups.
The composition of the human erythrocyte membrane (RBC) glycoprotein- and glycolipid-bound sialic acids of A, B, AB and O type donors was studied using a new method (Zanetta et al., Glycobiology 11 (2001) 663-676). In addition to Neu5Ac as the major compound, Kdn, Neu5,9Ac(2), Neu5,7Ac(2), Neu (de-N-acetylated-Neu5Ac), Neu5Ac8Me, Neu5Ac9Lt, Neu4,5Ac(2), Neu5,8Ac(2)9Lt and Neu5Ac8S were characterised. Among these different compounds, Neu5Ac8Me, Neu5Ac9Lt, Neu4,5Ac(2), Neu5,8Ac(2)9Lt and Neu5Ac8S have never been described and quantitatively determined before in human tissues or cells. Neu5Gc and its O-alkylated or O-acylated derivatives were not detected.
Bulai
Tatiana
T
Laboratoire de Glycobiologie Structurale et Fonctionnelle, CNRS Unité Mixte de Recherche No. 8576, Université des Sciences et Technologies de Lille, Bâtiment C9, 59655 Cedex, Villeneuve d'Ascq, France.
Bratosin
Daniela
D
Pons
Alexandre
A
Montreuil
Jean
J
Zanetta
Jean Pierre
JP
D000017:ABO Blood-Group System / Q000737:chemistry*; D004912:Erythrocytes / Q000737:chemistry / Q000502:physiology*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D019158:N-Acetylneuraminic Acid / Q000032:analysis / Q000097:blood*; D013400:Sugar Acids / Q000032:analysis / Q000097:blood
D000017:ABO Blood-Group System; D013400:Sugar Acids; C050008:3-deoxyglycero-galacto-nonulosonic acid; D019158:N-Acetylneuraminic Acid