[An exploration of the preventive effects on lanthanum chloride on enteral bacterial translocation in scalded rats].
OBJECTIVE
To explore the preventive effect of lanthanum chloride on enteral bacterial translocation in scalded rats.


METHODS
Ninety Sprague-Dawley (SD) rats were employed in the study and randomly divided into three groups, i.e. normal control (A), burn control (B) and treatment (C) groups. Plasmid PUC19 labelled by JM109 was transfected to Escherichia coli (E. coli), so that restriction endonuclease finger - print image spectrum analysis could be applied to the tracing and quantification of the translocation of E. coli from intestine to mesenteric lymph nodes (MLNs) and blood. The intestinal tissue contents of endotoxin (ET), nitric oxide (NO), nitric oxide synthase (NOS), malondialdehyde (MDA) and superoxide dismutase (SOD) were determined.


RESULTS
It was identified that the bacteria in MLNs and blood exhibited the same gene map with those from gastric gavage in B and C groups. But the bacterial quantity in MLNs in C group on 3 postburn day (PBD) was much lower than that in B group (P < 0.05). The intestinal MDA content in C group on 1 and 3 PBDs was obviously higher than that in B group (P < 0.05).


CONCLUSIONS
Bacteria (E. coli) could be translocated from gut to MLNs and blood, which could be evidently alleviated by lanthanum chloride by means of its bactericidal property, inhibition of NOS activity, so that NO production decreased, and its ability to increase SOD activity leading to less production of MDA.
Liu
Qiang
Q
Burn Center, The First Affiliated Hospital of Jiangxi Medical University, Nanchang 330006, Jiangxi Province, P.R. China.
Zhang
Yongmo
Y
Li
Guohui
G
Cao
Yong
Y
Hu
Qinghong
Q
Wu
Xieqing
X
Zhong
Xiaochun
X
Wang
Wen
W
Wang
Nianyun
N
D000818:Animals; D002056:Burns / Q000188:drug therapy* / Q000382:microbiology; D004731:Endotoxins / Q000097:blood / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D004927:Escherichia coli Infections / Q000097:blood / Q000382:microbiology / Q000517:prevention & control*; D005260:Female; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects / Q000382:microbiology; D007811:Lanthanum / Q000494:pharmacology*; D008198:Lymph Nodes / Q000187:drug effects / Q000382:microbiology; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D008643:Mesentery / Q000187:drug effects / Q000382:microbiology; D009569:Nitric Oxide / Q000097:blood / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000097:blood / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism
D004731:Endotoxins; C028521:lanthanum chloride; D009569:Nitric Oxide; D008315:Malondialdehyde; D007811:Lanthanum; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase