In vivo induction of apoptosis correlating with reduced infectivity during baculovirus infection.
Spodoptera frugiperda caterpillars were infected with a mutant of Autographa californica M nucleopolyhedrovirus lacking the antiapoptotic p35 gene. Viral infectivity, replication, and spread were substantially reduced compared to that of a control revertant virus. Terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling confirmed that apoptosis occurred in mutant-infected caterpillars, thus directly correlating reduced infectivity and in vivo induction of apoptosis.
Clarke
Thomas E
TE
Molecular, Cellular, and Developmental Biology Program, Division of Biology, Kansas State University, Manhattan, Kansas 66506, USA.
Clem
Rollie J
RJ
D000818:Animals; D017209:Apoptosis; D006458:Hemolymph / Q000821:virology; D020287:In Situ Nick-End Labeling; D017924:Nucleopolyhedroviruses / Q000472:pathogenicity*; D010641:Phenotype; D018411:Spodoptera / Q000821:virology*