Immunolocalization of PTHrP in prepubertal and pubertal testis of European bison.
The present study deals with immunohistochemical localization of PTHrP in prepubertal and pubertal testis of European bison. PTHrP immunoreactivity was observed in germinal cells in the testis of both prepubertal and pubertal animals. In calves, PTHrP was found in germinal cells, in seminiferous tubules lacking the lumen. The reaction was strong and regularly distributed within the cytoplasm. In adult animals, the reaction showed differentiation in spermatogenic cells. Some cells were strongly and diffusely stained, others exhibited weaker reaction of granular pattern. Sertoli cells and Leydig cells were PTHrP-negative in calves and adult animals.
Czykier
Elzbieta
E
Department of Histology and Embryology, Medical Academy, Białystok, Poland. czykier@amb.edu.pl
Zabel
Maciej
M
Surdyk-Zasada
Joanna
J
D000818:Animals; D016164:Bison / Q000254:growth & development*; D007150:Immunohistochemistry; D008297:Male; D044162:Parathyroid Hormone-Related Protein; D036361:Peptide Hormones / Q000032:analysis* / Q000276:immunology; D012671:Seminiferous Tubules / Q000737:chemistry; D012708:Sertoli Cells / Q000737:chemistry; D012741:Sexual Maturation; D013737:Testis / Q000033:anatomy & histology / Q000737:chemistry* / Q000254:growth & development*
D044162:Parathyroid Hormone-Related Protein; D036361:Peptide Hormones