Isolation and characterization of two types of actinophages infecting Streptomyces scabies MR13.
Two types of actinophages, phi S and phi L, were isolated from soil samples by using Streptomyces scabies MR13, a potato scab pathogen, as an indicator strain. The phages were partially characterized according to their physicochemical properties, plaques and particles morphology and their host-range. The host-range of these phages was narrow for phi S and wide for phi L. The adsorption rate constants of the phi S and phi L were 3.44 x 10(-9) and 3.18 x 10(-9) ml/min, and their burst sizes were 1.61 and 3.75 virions, respectively. One-step growth indicated that phi S and phi L have a latent period of 30 min followed by a rise period of 30 min. The temperate character of these phages was tested in other isolates of Streptomyces. Four of the phages (phi SS3, phi SS12, phi SS13 and phi SS17) were identified as temperate phages, since they were able to lysogenize SS3, SS12, SS13 and SS17. phi SS3, phi SS12 and phi SS13 were homoimmune, and they were heteroimmune with respect to phi SS17. The restriction barriers of lysogenic isolates (SS12, SS13 and SS17) interfered with the blockage of plaques formation by phages (phi SS12, phi SS13 or phi SS17) propagated on them, about 75% of lysogenic isolates had restriction systems. The exposure of the lysogenic isolates (SS12, SS13 and SS17) to UV-irradiation prevented the possible restriction barriers of these isolates, and these barriers could be overcome.
el-Sayed
A el-S
Ael-S
Department of Botany, Faculty of Science, Zagazig University, Zagazig, Egypt.
el-Didamony
G
G
Mansour
K
K
D001435:Bacteriophages / Q000302:isolation & purification* / Q000502:physiology / Q000528:radiation effects / Q000648:ultrastructure; D004279:DNA, Viral / Q000235:genetics; D008242:Lysogeny; D012988:Soil Microbiology; D013302:Streptomyces / Q000821:virology*; D014466:Ultraviolet Rays; D010948:Viral Plaque Assay
D004279:DNA, Viral