[Transfection of eucaryotic cells using cytochrome CYP450 2B6 gene: sensitivity to cyclophosphamide].
Sensitivity of 293 human epithelial kidney cells transfected by human cytochrome CYP450 gene to cyclophosphamide was investigated. Transfection was carried out by plasmid DNA containing CYP2B6 gene complexed with cationic liposomes. Liposomes were prepared from mixture of cationic lipids and cholesterol at different molar ratios. Experimental protocol included the following steps: transfection of epithelial kidney cells by complexes of plasmid DNA-cationic liposomes, clone selection in the medium with antibiotic Geneticin G418, selected clone harvesting and their treatment by cyclophosphamide as following cytotoxicity evaluation. It was shown that addition of 0.25 mM of cyclophosphamide resulted in death of 40-45% transfected cell population.
Kolomeĭchuk
S N
SN
Institute of Biomedical Chemistry RAMS, Lomonosov Moscow State Academy of Fine Chemical Technology, Moscow, Russia. sokolov@ibmh.msk.su
Abakumova
O Iu
OIu
Maslov
M A
MA
Kalashnikova
E A
EA
Morozova
N G
NG
Serebrennikova
G A
GA
Shvets
V I
VI
Sokolov
N N
NN
D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D002412:Cations; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003520:Cyclophosphamide / Q000494:pharmacology*; D065702:Cytochrome P-450 CYP2B6; D004361:Drug Tolerance; D004847:Epithelial Cells / Q000187:drug effects*; D006801:Humans; D007668:Kidney / Q000166:cytology; D008081:Liposomes; D010089:Oxidoreductases, N-Demethylating / Q000235:genetics*; D014162:Transfection
D018906:Antineoplastic Agents, Alkylating; D002412:Cations; D008081:Liposomes; D003520:Cyclophosphamide; D001189:Aryl Hydrocarbon Hydroxylases; C585599:CYP2B6 protein, human; D065702:Cytochrome P-450 CYP2B6; D010089:Oxidoreductases, N-Demethylating