title: KIF17 dynamics and regulation of NR2B trafficking in hippocampal neurons.
abstract: KIF17, a recently characterized member of the kinesin superfamily proteins, has been proposed to bind in vitro to a protein complex containing mLin10 (Mint1/X11) and the NR2B subunit of the NMDA receptors (NMDARs). In the mammalian brain, NMDARs play an important role in synaptic plasticity, learning, and memory. Here we present, for the first time, the dynamic properties of KIF17 and provide evidence of its function in the transport of NR2B in living mammalian neurons. KIF17 vesicles enter and move specifically along dendrites in a processive way, at an average speed of 0.76 microm/sec. These vesicles are effectively associated with extrasynaptic NR2B, and thus they transport and deliver NR2B subunits in dendrites. However, KIF17 does not seem to enter directly into postsynaptic regions. Cellular knockdown or functional blockade of KIF17 significantly impairs NR2B expression and its synaptic localization. Interestingly, the decrease in the number of synaptic NR2B subunits is followed by a parallel increase in the number of NR2A subunits at synapses. In contrast, upregulation of the expression level of NR2B, after treatment with the NMDAR antagonist D(-)-2-amino-5-phosphonopentanoic acid, simultaneously increases the expression level of KIF17. These observations concerning the downregulation or upregulation of KIF17 and NR2B reveal the probable existence of a shared regulation process between the motor and its cargo. Taken together, these results illustrate the complex mechanisms underlying the active transport and regulation of NR2B by the molecular motor KIF17 in living hippocampal neurons.
authors.affiliation: Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Laurent
authors.initials: L
authors.lastname: Guillaud
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Hirokawa
mesh_terms: D016475:3T3 Cells; D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D002352:Carrier Proteins / Q000032:analysis; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D016547:Kinesins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D007700:Kinetics; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D051379:Mice; D020409:Molecular Motor Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000032:analysis; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D021381:Protein Transport; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000032:analysis; D015854:Up-Regulation
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C513072:Apba1 protein, mouse; D001426:Bacterial Proteins; D002352:Carrier Proteins; C409642:KIF17 protein, mouse; D008164:Luminescent Proteins; D020409:Molecular Motor Proteins; C121001:NR2B NMDA receptor; D009419:Nerve Tissue Proteins; D016376:Oligonucleotides, Antisense; D016194:Receptors, N-Methyl-D-Aspartate; D011993:Recombinant Fusion Proteins; C054562:yellow fluorescent protein, Bacteria; D016547:Kinesins