Characterization of an integron carrying a new class D beta-lactamase (OXA-37) in Acinetobacter baumannii.
Integrons from a clinical strain of Acinetobacter baumannii highly resistant to beta-lactams have been analyzed. The largest (2.2 kb) contained three gene cassettes: an aacA4, an open reading frame of 417 nucleotides, and an OXA-type encoding gene. The oxa gene nucleotide sequence differed from that of the oxa-20 in 2 bp, one of the mutations being silent. The nonsilent mutationgenerated a substitution of glutamic acid for aspartic acid. The new OXA has been named OXA-37.
Navia
Margarita M
MM
Laboratori de Microbiologia, Institut Clinic Infeccions i Immunologia, Hospital Clinic i Provincial, Institut d'Investigacions Biomédiques August Pi I Sunyer (IDIBAPS), Universitat de Barcelona, Spain.
Ruiz
Joaquim
J
Vila
Jordi
J
D000151:Acinetobacter Infections / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D032023:Integrons / Q000235:genetics*; D007525:Isoelectric Focusing; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013030:Spain; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism*
D003062:Codon; C474923:beta-lactamase OXA-37; D001618:beta-Lactamases