Steroidogenic alterations in testes and sera of rats exposed to formulated Fenvalerate by inhalation.
Fenvalerate (Fen) is a synthetic pyrethroid, which is commonly used for destroying a variety of insect pests damaging several vegetable, fruit, and cotton crops. This insecticide is also used to mitigate household insects like flies, cockroaches, mosquitoes, and so forth. Human beings are exposed to formulated Fen preparations mostly by inhalation during spraying in fields for crop protection, for control of household insects, and also during handling and packaging at manufacturing plants. Limited online information is available regarding toxic effects of formulated Fen exposure on mammalian reproductive system. The present study has been undertaken to investigate male reproductive toxic effects of a formulated preparation of Fen (20% EC) particularly in relation to steroidogenic alterations in testes and sera of rats exposed by nose-only inhalation for (4 hours/day and five days a week) for three months. The results indicate significant reduction in the weight of testes, epididymal sperm counts, and sperm motility, along with decrease in marker testicular enzymes for testosterone biosynthesis viz. 17-beta-hydroxy steroid dehydrogenase (17-beta-HSD) and glucose-6-phosphate dehydrogenase (G6PDH), leading to net decrease in serum testosterone concentration in group of rats exposed to one-fifth LC50 of Fen (20% EC) by inhalation (4 hours/day, five days a week) subchronically for three months. These results for the first time indicate the role of testosterone in Fen (20% EC)-induced male reproductive toxicity of rats subchronically exposed by inhalation probably due to neuroendocrine-mediated phenomenon and hormone-disrupting property of the insecticide.
Mani
U
U
Inhalation Toxicology Laboratory, Industrial Toxicology Research Centre, PO Box 80, M.G. Marg, Lucknow 226 001, Uttar Pradesh, India. uthmani@rediffmail.com
Islam
F
F
Prasad
A K
AK
Kumar
P
P
Suresh Kumar
V
V
Maji
B K
BK
Dutta
K K
KK
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000280:Administration, Inhalation; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007306:Insecticides / Q000737:chemistry / Q000633:toxicity*; D007928:Lethal Dose 50; D008297:Male; D009570:Nitriles; D009929:Organ Size / Q000187:drug effects; D011722:Pyrethrins / Q000737:chemistry / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013737:Testis / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D013739:Testosterone / Q000097:blood*
D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D013739:Testosterone; D015067:17-Hydroxysteroid Dehydrogenases; D005954:Glucosephosphate Dehydrogenase; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase; C017690:fenvalerate