Expression of sorcin predicts poor outcome in acute myeloid leukemia.
Using a cDNA microarray 12 differentially expressed genes were identified in a multidrug resistant (MDR) cell line K562/A02. The differential expression of sorcin, which was one of the 12 genes, has been confirmed by Northern blot. To determine the clinical role of sorcin, we have measured its expression in leukemic blast cells of 65 acute myeloid leukemia (AML) patients by reverse transcriptase polymerase chain reaction (RT-PCR). Sorcin overexpression in AML patients was associated with poor clinical outcomes, the complete remission (CR) rate in sorcin- cases was significantly higher than that of sorcin+ cases (P<0.001). Furthermore, sorcin expression in AML patients was positively correlated with mdr1 expression (r=0.841, P<0.001). Combination of sorcin and mdr1 was related to clinical outcome too, cases with sorcin-/mdr1- had best response to induction chemotherapy. Our results indicated that sorcin might be one of the factors that contributes to drug resistance of AML patients.
Tan
Yaohong
Y
State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300020, China.
Li
Guangyao
G
Zhao
Chunhua
C
Wang
Jianxiang
J
Zhao
Hongguo
H
Xue
Yanping
Y
Han
Mingzhe
M
Yang
Chunzheng
C
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002648:Child; D018432:Drug Resistance, Multiple / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D007951:Leukemia, Myeloid / Q000175:diagnosis* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis; D016896:Treatment Outcome
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002135:Calcium-Binding Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C503113:SRI protein, human