title: Geobacillus toebii sp. nov., a novel thermophilic bacterium isolated from hay compost.
abstract: A thermophilic, spore-forming rod isolated from hay compost in Korea was subjected to a taxonomic study. The micro-organism, designated strain SK-1(T), was identified as being aerobic, Gram-positive, motile and rod-shaped. Growth of the isolate was observed at 45-70 degrees C (optimum 60 degrees C) and pH 6.0-9.0 (optimum pH 7.5). The G+C content of the genomic DNA was 43.9 mol%. Chemotaxonomic characteristics of the isolate included the presence of mesodiaminopimelic acid in the cell wall and iso-C15:0 and iso-C17:0 as the major cellular fatty acids. The predominant isoprenoid quinone was MK-7. The chemotaxonomic characteristics of strain SK-1(T) were the same as those of the genus Geobacillus. Phylogenetic analysis based on 16S rDNA sequences showed that strain SK-1(T) is most closely related to Geobacillus thermoglucosidasius. However, the phenotypic properties of strain SK-1(T) were clearly different from those of G. thermoglucosidasius. The level of DNA-DNA relatedness between strain SK-1(T) and the type strain of G. thermoglucosidasius was 27%. On the basis of the phenotypic traits and molecular systematic data, strain SK-1(T) represents a novel species within the genus Geobacillus, for which the name Geobacillus toebii sp. nov. is proposed. The type strain is strain SK-1(T) (= KCTC 0306BP(T) - DSM 14590(T)).
authors.forename: M H
authors.initials: MH
authors.lastname: Sung
authors.forename: H
authors.initials: H
authors.lastname: Kim
authors.forename: J W
authors.initials: JW
authors.lastname: Bae
authors.forename: S K
authors.initials: SK
authors.lastname: Rhee
authors.forename: C O
authors.initials: CO
authors.lastname: Jeon
authors.forename: K
authors.initials: K
authors.lastname: Kim
authors.forename: J J
authors.initials: JJ
authors.lastname: Kim
authors.forename: S P
authors.initials: SP
authors.lastname: Hong
authors.forename: S G
authors.initials: SG
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Yoon
authors.forename: Y H
authors.initials: YH
authors.lastname: Park
authors.forename: D H
authors.initials: DH
authors.lastname: Baek
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D016937:Gram-Positive Endospore-Forming Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006358:Hot Temperature; D007723:Korea; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D006109:Poaceae / Q000382:microbiology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D009626:Terminology as Topic
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S