Death style and respiratory burst of neutrophils in peripheral blood and pulmonary alveolus under endotoxemia in rats.
To study the difference of changes on apoptosis, necrosis and respiratory burst of the polymorphonuclear neutrophils (PMN) in endotoxemia rat model. LPS (O(55)B(5), 5 mg/kg) was injected into abdominal cavity of 20 random normal Wistar rat. 2, 4, 8 and 12 hours after injection, the changes of apoptosis, necrosis and respiratory burst of the rats between PMN from the peripheral blood and from the bronchoalveolar lavage fluid were observed using the flow cytometer. At the same time, 5 uninjected rats were taken as control. The results demonstrated that the quantity proportions of apoptosis of PMN between the peripheral blood PMN and the bronchoalveolar lavage fluid PMN in rat's endotoxemia were similar. However, comparison with the uninjected LPS rat, the necrosis of peripheral blood PMN obviously increased and the respiratory burst capacity was clearly inhibited. Contrarily, the necrosis of bronchoalveolar lavage fluid PMN obviously decreased and the respiratory burst obviously increased in the injecting LPS rat. It was concluded that the necrosis and apoptosis displayed differently between the pulmonary and peripheral blood PMNs in endotoxemia. Under state of inflammation, the surviving PMN in tissue increased and kept the activated state due to tissue injury.
Li
Shuang-Ding
SD
Medicine College of Xi'an Jiaotong University, Xi'an 710061, China.
Liu
Ren
R
He
Chun-Yang
CY
Xiao
Nan
N
Tian
Kun-Lun
KL
D000818:Animals; D017209:Apoptosis; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D019446:Endotoxemia / Q000097:blood*; D009336:Necrosis; D009504:Neutrophils / Q000502:physiology*; D011650:Pulmonary Alveoli / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D016897:Respiratory Burst