title: Express primer tool for high-throughput gene cloning and expression.
abstract: High-throughput approaches for gene cloning and expression require the development of new nonstandard tools for molecular biologists and biochemists. We introduce a Web-based tool to design primers specifically for the generation of expression clones for both laboratory-scale and high-throughput projects. The application is designed not only to allow the user complete flexibility to specify primer design parameters but also to minimize the amount of manual intervention needed to generate a large number of primers for the simultaneous amplification of multiple target genes.
authors.affiliation: Argonne National Laboratory, Argonne, IL, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Yoon
authors.forename: P D
authors.initials: PD
authors.lastname: Laible
authors.forename: M
authors.initials: M
authors.lastname: Gu
authors.forename: H N
authors.initials: HN
authors.lastname: Scott
authors.forename: F R
authors.initials: FR
authors.lastname: Collart
mesh_terms: D000465:Algorithms; D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers / Q000138:chemical synthesis / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D015195:Drug Design; D001330:Electronic Data Processing; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D012984:Software; D013696:Temperature
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial