title: CCR6 is a functional chemokine receptor that serves to identify select B-cell non-Hodgkin's lymphomas.
abstract: Several recent studies have revealed important contributions of chemokines and their receptors to the development and progression of both hematopoietic and nonhematopoietic neoplasms. The chemokine receptor CCR6 is unusual in that it mediates leukocyte chemotaxis in response to a single chemokine, CCL20 (macrophage inhibitory factor-3alpha), as well as in response to a family of antimicrobial peptides termed "beta-defensins." CCR6 is critical for mucosal immunity, and expression of the receptor is tightly regulated on hematopoietic cells. Here we characterize the expression of CCR6 on B cells and B-cell non-Hodgkin's lymphomas. We demonstrate that CCR6 expression is limited to cells comprising the mantle and marginal zones of the secondary lymphoid tissues and serves to identify the majority of mantle cell, marginal zone, and mucosa-associated lymphoid tissue lymphomas. Furthermore, we show that CCR6 serves as a functional chemokine receptor when expressed by neoplastic cells. Finally, we establish that the cognate ligand for CCR6 is present on mucosal epithelium infiltrated by neoplastic cells in select extranodal lymphomas. Thus, CCR6 is a useful new marker identifying a subset of B-cell non-Hodgkin's lymphomas and likely contributes to the localization of select extranodal lymphomas at mucosal sites.
authors.affiliation: Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Rodig
authors.forename: Dan
authors.initials: D
authors.lastname: Jones
authors.forename: Aliakbar
authors.initials: A
authors.lastname: Shahsafaei
authors.forename: David M
authors.initials: DM
authors.lastname: Dorfman
mesh_terms: D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000032:analysis*; D054418:Chemokine CCL20; D019742:Chemokines, CC / Q000032:analysis; D002634:Chemotaxis, Leukocyte; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000737:chemistry; D008221:Lymphoid Tissue / Q000737:chemistry; D016393:Lymphoma, B-Cell / Q000737:chemistry*; D018442:Lymphoma, B-Cell, Marginal Zone / Q000737:chemistry; D019402:Macrophage Inflammatory Proteins / Q000032:analysis; D054399:Receptors, CCR6; D019707:Receptors, Chemokine / Q000032:analysis* / Q000502:physiology; D012680:Sensitivity and Specificity; D013154:Spleen / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; C108355:CCL20 protein, human; C106311:CCR6 protein, human; D054418:Chemokine CCL20; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins; D054399:Receptors, CCR6; D019707:Receptors, Chemokine