Use of polymerase chain reaction for diagnosis of disseminated adenovirus infection.
We report a fatal case of disseminated adenovirus infection with fulminant hepatic failure in an 8-month-old child after peripheral blood stem cell transplantation. The virus was identified in blood, urine, respiratory aspirate and stool samples and was typed as adenovirus type 2 through PCR and sequencing of part of the hexon gene, with the use of degenerated consensus primers.
Rector
Annabel
A
Laboratory of Clinical Virology, Rega Institute for Medical Research, University of Leuven, Belgium.
Azzi
Nadira
N
Liesnard
Corinne
C
Zlateva
Kalina
K
Van Beers
Daniele
D
Snoeck
Robert
R
Van Ranst
Marc
M
D000257:Adenoviridae Infections / Q000175:diagnosis* / Q000628:therapy; D000260:Adenoviruses, Human / Q000302:isolation & purification*; D004279:DNA, Viral / Q000097:blood*; D018450:Disease Progression; D017809:Fatal Outcome; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D006801:Humans; D007223:Infant; D015479:Leukemia, Myelomonocytic, Acute / Q000175:diagnosis / Q000276:immunology / Q000628:therapy; D008297:Male; D009102:Multiple Organ Failure; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D014766:Viremia / Q000175:diagnosis* / Q000628:therapy
D004279:DNA, Viral