Developing transgenic Arabidopsis plants to be metal-specific bioindicators.
Deoxyribonucleic acid (DNA) microarrays provide a means to assess genome-wide expression patterns after exposure of an organism to different xenobiotics. Potential uses for this technology include identification of unknown toxicants, assessment of toxicity of new compounds, and characterization of the cellular mechanisms of toxicant action. Here we describe another use of DNA microarrays in toxicant-specific gene discovery. Combining results from two DNA microarray experiments, we have identified genes from the model plant Arabidopsis thaliana that are induced in response to one but not other heavy metals. The promoters of these genes should be useful in developing metal-specific transgenic biomonitors. To test this idea, we have fused the promoter of one of the newly identified Ni-inducible genes (AHB1) to the beta-glucuronidase (GUS) reporter gene. Arabidopsis plants containing the AHBI::GUS transgene show reporter gene activity when they are grown on media containing Ni but not when grown on media containing Cd, Cu, Zn, or without added metals. Thus, this approach has resulted in the creation of a transgenic strain of Arabidopsis that can report on the presence and concentration of Ni in plant growth media. Such transgenic models can serve as cheap and efficient biomonitors of bioavailable heavy metal contamination in soils and sediments.
Krizek
Beth A
BA
Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208, USA. krizek@sc.edu
Prost
Valerie
V
Joshi
Ratan Mani
RM
Stoming
Terry
T
Glenn
Travis C
TC
D001682:Biological Availability; D015415:Biomarkers / Q000032:analysis; D019607:Brassicaceae / Q000235:genetics*; D004784:Environmental Monitoring / Q000379:methods; D019216:Metals, Heavy / Q000493:pharmacokinetics / Q000633:toxicity*; D020411:Oligonucleotide Array Sequence Analysis; D030821:Plants, Genetically Modified
D015415:Biomarkers; D019216:Metals, Heavy