Genetic specification of axonal arbors: atonal regulates robo3 to position terminal branches in the Drosophila nervous system.
Drosophila sensory neurons form distinctive terminal branch patterns in the developing neuropile of the embryonic central nervous system. In this paper we make a genetic analysis of factors regulating arbor position. We show that mediolateral position is determined in a binary fashion by expression (chordotonal neurons) or nonexpression (multidendritic neurons) of the Robo3 receptor for the midline repellent Slit. Robo3 expression is one of a suite of chordotonal neuron properties that depend on expression of the proneural gene atonal. Different features of terminal branches are separately regulated: an arbor can be shifted mediolaterally without affecting its dorsoventral location, and the distinctive remodeling of one arbor continues as normal despite this arbor shifting to an abnormal position in the neuropile.
Zlatic
Marta
M
Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, United Kingdom.
Landgraf
Matthias
M
Bate
Michael
M
D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure*; D051792:Basic Helix-Loop-Helix Proteins; D002450:Cell Communication / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology* / Q000254:growth & development; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D005260:Female; D007839:Functional Laterality / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D020439:Growth Cones / Q000378:metabolism / Q000648:ultrastructure; D007814:Larva / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009420:Nervous System / Q000166:cytology / Q000196:embryology* / Q000254:growth & development; D009421:Nervous System Malformations / Q000235:genetics; D009473:Neuronal Plasticity / Q000235:genetics; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D000097905:Roundabout Proteins
D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011971:Receptors, Immunologic; C082142:ato protein, Drosophila; C110665:comm protein, Drosophila; C421244:robo3 protein, Drosophila; D000097905:Roundabout Proteins; C066819:sli protein, Drosophila