title: Simultaneous quantification of citalopram, clozapine, fluoxetine, norfluoxetine, maprotiline, desmethylmaprotiline and trazodone in human serum by HPLC analysis.
abstract: We describe an analytical procedure for the simultaneous quantification of citalopram (seropram), clozapine (leponex), fluoxetine (fluctine), norfluoxetine, maprotiline (ludiomil), desmethylmaprotiline and trazodone (trittico) in human serum within a period of 11.5 minutes using reversed phase HPLC. After 2 liquid/liquid extractions in the sample preparation phase, the drugs and metabolites were separated on a C18 column using a mobile phase consisting of acetonitrile/buffer (30/70, v:v) at 70 degrees C, a flow rate of 1.5 m/min and haloperidol as internal standard. Absorption and native fluorescence signals of the eluted compounds were detected simultaneously at 260 nm and 227/300 nm (excitation/emission), respectively. The calibration ranges for citalopram, clozapine, fluoxetine, norfluoxetine, maprotiline, and desmethylmaprotiline ranged from 50-400 microg/l and for trazodone from 50-3,200 microg/l. The CVs varied between 0.6% and 5.5% (within-run) and between 3.2% and 7.1% (between-run). Recoveries were > 90% for all pharmaceuticals. We noticed no interferences from several commonly used drugs.
authors.affiliation: Medical Central Laboratory, Feldkirch, Austria. rwaschgler@mzl.at
authors.forename: R
authors.initials: R
authors.lastname: Waschgler
authors.forename: M R
authors.initials: MR
authors.lastname: Hubmann
authors.forename: A
authors.initials: A
authors.lastname: Conca
authors.forename: W
authors.initials: W
authors.lastname: Moll
authors.forename: P
authors.initials: P
authors.lastname: König
mesh_terms: D018687:Antidepressive Agents, Second-Generation / Q000097:blood*; D014150:Antipsychotic Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D015283:Citalopram / Q000097:blood; D003024:Clozapine / Q000097:blood; D005473:Fluoxetine / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D008376:Maprotiline / Q000031:analogs & derivatives* / Q000097:blood; D014196:Trazodone / Q000097:blood
chemical_list: D018687:Antidepressive Agents, Second-Generation; D014150:Antipsychotic Agents; D005473:Fluoxetine; D015283:Citalopram; D008376:Maprotiline; C027056:desmethylmaprotiline; D003024:Clozapine; C036139:norfluoxetine; D014196:Trazodone