Multi-analyte analysis system using an antibody-based biochip.
A multi-analyte detection system using a unique antibody (Ab) biochip is described. The Ab-based biochip, also referred to as the protein biochip, uses a sensor array based on a complementary metal oxide silicon (CMOS) integrated circuit. The Ab-biochip has a sampling platform of four-by-four microarrays of antibodies deposited onto a Nylon membrane substrate. The micro-arrayed antibodies can be interrogated simultaneously or sequentially using the biochip sensing array detector with the use of a diffractive optical element illuminating each antibody spot individually. The usefulness of the Ab biochip is illustrated by the measurements of immunoglobulin G (IgG) used as the model analyte system. The detection limit for Cy5-labeled IgG molecules was 13 pg.
Moreno-Bondi
Maria Cruz
MC
Department of Analytical Chemistry, Facultad de Quimica, Universidad Complutense, 28040 Madrid, Spain.
Alarie
Jean Pierre
JP
Vo-Dinh
Tuan
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000936:Antigen-Antibody Complex / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002138:Calibration; D004867:Equipment Design; D006041:Goats; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011817:Rabbits; D012756:Sheep
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G