Bipolar localization of putative photoreceptor protein for phototaxis in thermophilic cyanobacterium Synechococcus elongatus.
We identified an open reading frame from a database of the entire genome of Synechococcus elongatus, the product of which was very similar to pixJ1, which was proposed as photoreceptor gene for phototaxis in Synechocystis sp. PCC6803 [Yoshihara et al. (2000) Plant Cell Physiol. 41: 1299]. The mRNA of S. elongatus pixJ (SepixJ) was expressed in vivo as a part of the product of an operon. SePixJ was detected exclusively in the membrane fraction after cell fractionation. Immunogold labeling of SePixJ in ultra-thin sections indicated that it existed only in both ends of the rod-shaped cell; probably bound with the cytoplasmic membrane.
Kondou
Youichi
Y
Graduate School of Integrated Science, Yokohama City University, Seto 22-2, Kanazawa-ku, Yokohama, 236-0027 Japan.
Mogami
Norifumi
N
Hoshi
Fumiko
F
Kutsuna
Shinsuke
S
Nakazawa
Miki
M
Sakurai
Tetsuya
T
Matsui
Minami
M
Kaneko
Takakazu
T
Tabata
Satoshi
S
Tanaka
Ichiro
I
Manabe
Katsushi
K
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D000458:Cyanobacteria / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects; D015964:Gene Expression Regulation, Bacterial / Q000528:radiation effects; D008027:Light; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D020283:Photoreceptors, Microbial / Q000235:genetics* / Q000378:metabolism; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism / Q000528:radiation effects; D010834:Phytochrome / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000528:radiation effects; D013696:Temperature
D001426:Bacterial Proteins; D020283:Photoreceptors, Microbial; D045322:Photosynthetic Reaction Center Complex Proteins; D010834:Phytochrome