Involvement of acetylated tubulin in the regulation of Na+,K+ -ATPase activity in cultured astrocytes.
The results presented support the view that the modulation of Na(+),K(+)-ATPase activity in living cells involves the association/dissociation of acetylated tubulin with the enzyme. We found that the stimulation of Na(+),K(+)-ATPase activity by L-glutamate correlates with decreased acetylated tubulin quantity associated with the enzyme. The effect of L-glutamate was abolished by the glutamate transporter inhibitor DL-threo-beta-hydroxyaspartate but was not affected by either specific agonists or antagonists. The effect of L-glutamate seems to be mediated by Na(+) entry resulting from glutamate transport, since the Na(+) ionophore monensin produced stimulation of Na(+),K(+)-ATPase activity with concomitant decrease of acetylated tubulin quantity associated with the enzyme.
Casale
Cesar H
CH
Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
Previtali
Gabriela
G
Barra
Héctor S
HS
D000107:Acetylation; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D051379:Mice; D008985:Monensin / Q000494:pharmacology; D016202:N-Methylaspartate / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects / Q000378:metabolism*; D014404:Tubulin / Q000187:drug effects / Q000378:metabolism*; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology
D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D007476:Ionophores; D014404:Tubulin; C011274:3-hydroxyaspartic acid; D001224:Aspartic Acid; D018698:Glutamic Acid; D016202:N-Methylaspartate; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D008985:Monensin; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose