MTO: the second member of a Drosophila dual copper-thionein system.
Drosophila MTO metal binding features were analyzed for comparison with MTN, the paralogous Drosophila metallothionein, and to classify MTO as either zinc- or copper-thionein. This was achieved by a combination of in vivo, in vitro and in silico methodologies. All the results unambiguously classified MTO as a second Drosophila copper-thionein, putting Drosophila forward as the only metazoan in which any zinc-thionein has still to be reported. Interestingly, experimental data only showed minor differences in the coordinative behavior of both MTs, but provided a characteristic spectroscopic fingerprint, revealing the possible binding of chloride anions in certain metal-MTO aggregates.
Domenech
J
J
Departament de Genètica, Facultat de Biologia, Av. Diagonal 645, Universitat de Barcelona, E-08028, Barcelona, Spain.
Palacios
O
O
Villarreal
L
L
González-Duarte
P
P
Capdevila
M
M
Atrian
S
S
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003300:Copper / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism; D008668:Metallothionein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010802:Phylogeny; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015032:Zinc / Q000737:chemistry
D018076:DNA, Complementary; D029721:Drosophila Proteins; D011994:Recombinant Proteins; D003300:Copper; D008668:Metallothionein; D015032:Zinc