[Involvement of PHO80 and PHO85 genes in Saccharomyces cerevisiae ion tolerance].
PHO85 is a versatile gene in Saccharomyces cerevisiae, which is involved in metabolism of inorganic phosphate and usage of carbon source, accumulation of glycogen, regulation of protein stability and cell cycle control. The viability of wild type budding yeast strain YPH499 and its derivative pho85Delta mutant, pho80   mutant, and pap1(pcl-7)Delta mutant in different cations were investigated and their tolerance to the cations(LC(50)) was measured. The results showed that the deletion of PHO85 or PHO80 gene both increased sensibility of Sacchromyces cerevisiae to ions K(+), Mg(2+), Zn(2+), Ca(2+) and Mn(2+), while the deletion of pap1(pcl-7) gene did not lead to such phenotype. The difference between the patterns of relative growth curve of the mutants and wild type strain in the above ions also implied that PHO80 was the unique PCLs in complex with PHO85 CDK, that were contributed to K(+) and Mg(2+) ion homeostasis control and there were some other PCLs besides PHO80 that were involved in Zn(2+), Ca(2+) and Mn(2+) tolerance regulation as cyclin of PHO85 CDK. Furthermore, the amount of the total cellular calcium of pho85Delta mutant, pho80Delta mutant and YPH499 indicated that the ability of calcium accumulation of pho85   mutant and pho80Delta mutant was impaired.
Mao
Xi-Cheng
XC
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China.
Xia
Yu-Lei
YL
Hu
Ya-Fang
YF
Lu
Chang-De
CD
D002118:Calcium / Q000378:metabolism; D002122:Calcium Chloride / Q000494:pharmacology; D002412:Cations / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D002712:Chlorides / Q000494:pharmacology; D019327:Copper Sulfate / Q000494:pharmacology; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000502:physiology*; D016213:Cyclins / Q000235:genetics / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D017353:Gene Deletion; D015636:Magnesium Chloride / Q000494:pharmacology; D017895:Manganese Compounds / Q000494:pharmacology; D009154:Mutation; D000073718:Pancreatitis-Associated Proteins; D011189:Potassium Chloride / Q000494:pharmacology; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D019287:Zinc Sulfate / Q000494:pharmacology
D002412:Cations; D002712:Chlorides; D016213:Cyclins; D017895:Manganese Compounds; C066824:PHO80 protein, S cerevisiae; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D015636:Magnesium Chloride; D011189:Potassium Chloride; D019287:Zinc Sulfate; D018844:Cyclin-Dependent Kinases; C058605:PHO85 protein, S cerevisiae; D019327:Copper Sulfate; D002122:Calcium Chloride; C025340:manganese chloride; D002118:Calcium