[Research of the anti-tumor effect of human endostatin mediated by recombinant adeno-associated virus].
OBJECTIVE
It was reported that endostatin could inhibit tumor angiogenesis, then inhibit the growth and metastasis of tumor. However, there was few report about the treatment usage of endostatin. This study was designed to explore the effect of endostatin mediated with recombinant adeno-associated virus(rAAV) on tumor growth and metastasis.


METHODS
To obtain the endostatin gene complete cDNA by RT-PCR, and clone it into the plasmid pSNAV and package the recombinant rAAV-SS-endostatin; to analyze its anti-tumor effect through animal experiments.


RESULTS
In the B16F10 melanoma tumor model, C57BL/6 mice were inoculated with 10(11)TU rAAV-SS-endostatin i.m. injection, which the inhibition rate was 57.1%; In the lung metastatic model, the inhibition rate of metastases was 70.7%.


CONCLUSIONS
rAAV-SS-endostatin can effectively inhibit tumor growth and metastasis.
Lu
Guo-qiu
GQ
State Key Laboratory of Molecular Oncology, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100021, P. R. China.
Zha
Yuan-yuan
YY
Wu
Xiao-bing
XB
Yang
Jian-guo
JG
Zhang
Xue-yan
XY
Fu
Ming
M
Liang
Xiao
X
Wu
Min
M
Lin
Chen
C
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D003094:Collagen / Q000235:genetics / Q000627:therapeutic use*; D000229:Dependovirus / Q000235:genetics; D004195:Disease Models, Animal; D043169:Endostatins; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000517:prevention & control*; D010446:Peptide Fragments / Q000235:genetics / Q000627:therapeutic use*
D000970:Antineoplastic Agents; D043169:Endostatins; D010446:Peptide Fragments; D003094:Collagen