[Refolding of recombinant proteins in vitro].
Refolding of recombinant proteins is an important event in the downstream process of genetic engineering. On the basis of the mechanism of protein folding in vitro, general strategy of recombinant protein refolding is postulated, and major developments in recent years on this field are reviewed, including: molecular chaperone mediated refolding, detergent-assisted refolding, protein refolding in reverse micelle, addition of folding enhancer and methods for the formation of disulfide bond during protein refolding.
Long
J Y
JY
Department of Biochemistry, Beijing Institute of Basic Medical Sciences, Beijing 100850.
Wang
H X
HX
D000818:Animals; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D017510:Protein Folding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D011994:Recombinant Proteins