Human mitochondrial transcription factor B1 methylates ribosomal RNA at a conserved stem-loop.
Human mitochondrial transcription factor B1 (h-mtTFB1) has an unprecedented relationship to RNA methyltransferases. Here, we show that this protein methylates a conserved stem-loop in bacterial 16S rRNA and that the homologous sequence in the human mitochondrial 12S molecule is similarly modified. Thus, h-mtTFB1 appears to be dual-function protein, acting both as a transcription factor and an rRNA-modification enzyme.
Seidel-Rogol
Bonnie L
BL
Department of Biochemistry, Rollins Research Center, Emory University School of Medicine, Atlanta, Georgia 30322-3050, USA.
McCulloch
Vicki
V
Shadel
Gerald S
GS
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D017124:Conserved Sequence; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D017353:Gene Deletion; D006801:Humans; D008745:Methylation; D008780:Methyltransferases / Q000235:genetics; D008928:Mitochondria / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D051720:Upstream Stimulatory Factors
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; C066779:B1 factor, Xenopus; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C075526:RNA, ribosomal, 12S; D014157:Transcription Factors; D051720:Upstream Stimulatory Factors; D008780:Methyltransferases; C047346:16S rRNA (adenine(1518)-N(6)-adenine(1519)-N(6))-dimethyltransferase; C100305:kasugamycin