title: SseA, a 3-mercaptopyruvate sulfurtransferase from Escherichia coli: crystallization and preliminary crystallographic data.
abstract: SseA, the translation product of the Escherichia coli sseA gene, is a 31 kDa protein endowed with 3-mercaptopyruvate:cyanide sulfurtransferase activity in vitro. As such, SseA is the prototype of a sulfurtransferase subfamily distinguished from the better known rhodanese sulfurtransferases, which display thiosulfate:cyanide sulfurtransferase activity. The physiological role of the two homologous enzyme families, whose catalytic activity is centred on a reactive invariant cysteine, is a matter of debate. In this framework, the forthcoming crystal structure analysis of SseA will be based on the tetragonal crystal form (space group P4(1) or P4(3)) reported here, with unit-cell parameters a = b = 150.2, c = 37.9 A.
authors.affiliation: Dipartimento di Fisica, infM, Centro di Eccellenza per la Ricerca Biomedica, Universita' di Genova, Via Dodecaneso 33, 16146 Genova, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Spallarossa
authors.forename: Aristodemo
authors.initials: A
authors.lastname: Carpen
authors.forename: Fabio
authors.initials: F
authors.lastname: Forlani
authors.forename: Silvia
authors.initials: S
authors.lastname: Pagani
authors.forename: Martino
authors.initials: M
authors.lastname: Bolognesi
authors.forename: Domenico
authors.initials: D
authors.lastname: Bordo
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013466:Sulfurtransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013884:Thiosulfate Sulfurtransferase / Q000737:chemistry / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D013466:Sulfurtransferases; D013884:Thiosulfate Sulfurtransferase; C021550:3-mercaptopyruvate sulphurtransferase