title: Peripheral blood-derived bovine dendritic cells promote IgG1-restricted B cell responses in vitro.
abstract: Regulation of humoral responses involves multiple cell types including the requirements for cognate interactions between T and B cells to drive CD40-dependent responses to T-dependent antigens. A third cell type has also been shown to play an essential role, the dendritic cell (DC). We demonstrate that bovine peripheral blood-derived (PB)-DC are similar in function to features described for human interstitial DC including the production of signature type 2 cytokines [interleukin (IL)-13, IL-10]. PB-DC express moderate-to-high costimulatory molecule expression, and major histocompatibility complex class II is negative for CD14 expression and has low or no expression of CD11c. Consistent with the interstitial phenotype is the ability of PB-DC to influence B cell activation and differentiation via direct expression of CD40L and type 2 cytokines. Collectively, these results suggest that direct B cell-DC interactions may promote an immunoglobulin-isotype expression pattern consistent with type 2 responses, independent of direct T cell involvement.
authors.affiliation: Department of Veterinary Pathobiology, Program for Prevention of Animal Infectious Diseases, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Anna A
authors.initials: AA
authors.lastname: Bajer
authors.forename: David
authors.initials: D
authors.lastname: Garcia-Tapia
authors.forename: Kimberly R
authors.initials: KR
authors.lastname: Jordan
authors.forename: Karen M
authors.initials: KM
authors.lastname: Haas
authors.forename: Dirk
authors.initials: D
authors.lastname: Werling
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Howard
authors.forename: D Mark
authors.initials: DM
authors.lastname: Estes
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D001773:Blood Cells / Q000166:cytology; D023201:CD40 Ligand / Q000032:analysis / Q000235:genetics; D002417:Cattle; D018920:Coculture Techniques; D016207:Cytokines / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology*; D016130:Immunophenotyping; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D016207:Cytokines; D007074:Immunoglobulin G; D012333:RNA, Messenger; D023201:CD40 Ligand