title: [The role of Kupffer cells on the postburn production of TNFalpha, IL-1beta and IL-6 in severely scalded rats].
abstract: OBJECTIVE
  To observe the role of Kupffer cells in the postburn production of TNFalpha, IL-1beta and IL-6 in severely scalded rats.
  
  
  METHODS
  (1) The production of TNFalpha, IL-1beta and IL-6 from rat Kupffer cells stimulated by burn serum was observed. (2) The postburn change in the expression of cytokine mRNA from rat Kupffer cells was monitored. (3) The change in the plasma cytokine contents in scalded rats was determined after the application of gadolinium chloride, a specific inhibitor of Kupffer cells.
  
  
  RESULTS
  Kupffer cells could be stimulated by burn serum to release cytokines TNFalpha, IL-1beta and IL-6. The mRNA expression of TNFalpha, IL-1beta and IL-6 from rat Kupffer cells increased significantly after injury. But the postburn plasma levels of TNFalpha, IL-1beta and IL-6 decreased obviously to 34.71%, 36.99% and 33.7% of those in scalding group, respectively, after the Kupffer cell activity was inhibited.
  
  
  CONCLUSIONS
  The plasma cytokines, i.e. TNFalpha, IL-1beta and IL-6, were primarily produced from Kupffer cells after injury in scalded rats, initiated by TNFalpha, IL-1beta and IL-6 mRNA transcription.
authors.affiliation: Department of Burns, Chang Hai Hospital, The Second Military Medical University, Shanghai, 200433 P.R. China.
authors.forename: Guangyi
authors.initials: G
authors.lastname: Wang
authors.forename: Jianguang
authors.initials: J
authors.lastname: Tian
authors.forename: Hongtai
authors.initials: H
authors.lastname: Tang
authors.forename: Shihui
authors.initials: S
authors.lastname: Zhu
authors.forename: Jingning
authors.initials: J
authors.lastname: Huan
authors.forename: Shengde
authors.initials: S
authors.lastname: Ge
authors.forename: Zhaofan
authors.initials: Z
authors.lastname: Xia
mesh_terms: D000818:Animals; D002056:Burns / Q000276:immunology* / Q000378:metabolism; D005682:Gadolinium / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D007728:Kupffer Cells / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D005682:Gadolinium; C038958:gadolinium chloride