title: [Screening of short peptides that bind specifically to osteosarcoma cells by phage-displayed peptide library].
abstract: OBJECTIVE
  To obtain short peptides which bind specifically to osteosarcoma cells os-732 by means of screening from 12 peptide libraries.
  
  
  METHODS
  Osteosarcoma cells os-732 were used as the target cells and osteoblasts as the absorber cells for subtraction biopanning from a 12-mer peptide phage-display library. After 3 rounds of screening, the positive phage clones were identified by cell enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, and the amino acid sequences were deduced by DNA sequencing.
  
  
  RESULTS
  Nine positive phage clones screened out of 20 clones showed specific binding with osteosarcoma os-732, but no conserved motif was found in these peptides.
  
  
  CONCLUSIONS
  The specific peptides screened from the phage library may be used as potential candidates as ligands for tumor-targeting therapy. The results also suggested that there are different epitopes on the surface of tumor cells.
authors.affiliation: Department of Rachidial Diseases, Nanfang Hospital, China. ghzhu68@fimmu.edu.cn
authors.forename: Guo-hong
authors.initials: GH
authors.lastname: Zhu
authors.forename: Bei-yi
authors.initials: BY
authors.lastname: Liu
authors.forename: Shan-gen
authors.initials: SG
authors.lastname: Zheng
authors.forename: Hai
authors.initials: H
authors.lastname: Lü
authors.forename: Zhan-jun
authors.initials: ZJ
authors.lastname: Shi
mesh_terms: D000595:Amino Acid Sequence; D001859:Bone Neoplasms / Q000378:metabolism*; D017124:Conserved Sequence; D006801:Humans; D007150:Immunohistochemistry; D012516:Osteosarcoma / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*
chemical_list: D019151:Peptide Library; D010455:Peptides