Secretion of granulocyte chemotactic protein-2 by cultured human endometrial stromal cells.
OBJECTIVE
To evaluate the expression of granulocyte chemotactic protein-2 (GCP-2) in human endometrial stromal cells.


METHODS
The effects of interleukin (IL)-1alpha, IL-1beta, tumor necrosis factor (TNF)-alpha, TNF-beta, interferon (IFN)-gamma, and lipopolysaccharide (LPS) on the production of GCP-2 by endometrial stromal cells were investigated.


METHODS
Research laboratory at a medical university.


METHODS
Eight endometrial specimens in the late proliferative phase were used.


METHODS
Endometrial stromal cells were incubated for 24 hours with IL-1alpha, IL-1beta, TNF-alpha, TNF-beta, IFN-gamma, and LPS.The concentration of GCP-2 in the culture media was measured using an enzyme-linked immunosorbent assay (ELISA).


RESULTS
A small amount of GCP-2 was detected in the culture media of unstimulated endometrial stromal cells. The production of GCP-2 by endometrial stromal cells was stimulated with IL-1alpha, IL-1beta, TNF-alpha, TNF-beta, and LPS in a dose-dependent manner. Interferon-gamma did not affect GCP-2 production by these cells.


CONCLUSIONS
These results suggest that GCP-2 is an additional ELR(+)-CXC chemokine expressed in endometrial stromal cells. The modulation of GCP-2 concentrations in the local environment may contribute to the normal and pathological processes of human reproduction by regulating the neutrophil trafficking in the endometrium.
Mine
Shinichiro
S
Department of Obstetrics and Gynecology, Oita Medical University, Oita, Japan.
Nasu
Kaei
K
Fukuda
Junichiro
J
Sun
Bing
B
Miyakawa
Isao
I
D002478:Cells, Cultured; D054427:Chemokine CXCL6; D019743:Chemokines, CXC / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000737:chemistry; D004717:Endometrium / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008233:Lymphotoxin-alpha / Q000494:pharmacology; D017154:Stromal Cells / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
C515503:CXCL6 protein, human; D054427:Chemokine CXCL6; D019743:Chemokines, CXC; D017077:Culture Media, Conditioned; D007375:Interleukin-1; D008070:Lipopolysaccharides; D008233:Lymphotoxin-alpha; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma