title: Trends in contact lens-associated microbial keratitis in Southern India.
abstract: OBJECTIVE
  To review the microbiologic profile, clinical course, treatment, and outcome in patients with contact lens-associated microbial keratitis in the setting of a tertiary eye care center in a developing country in the Tropics.
  
  
  METHODS
  Retrospective noncomparative case series selected from an ongoing prospective series.
  
  
  METHODS
  Twenty-eight subjects examined in the contact lens clinic of L. V. Prasad Eye Institute, Hyderabad, south India, between February 1991 and September 2000, who presented with corneal stromal infiltrate on slit-lamp examination, were included in the study.
  
  
  METHODS
  All patients underwent standard diagnostic microbiologic evaluation and smear and culture-guided topical antimicrobial therapy. Penetrating keratoplasty was performed in some patients.
  
  
  METHODS
  Culture results and clinical response to antimicrobial therapy.
  
  
  RESULTS
  Twenty eight (0.11%) of 23,889 contact lens-wearing patients presented with laboratory-proven infectious keratitis; this constituted 0.56% of 4967 corneal ulcer patients seen during the same period. Most (15/28) of the patients wore soft contact lenses on a daily basis. Rigid gas-permeable lenses were worn by six patients, therapeutic bandage contact lenses by four, and Silsoft lenses by four. Laboratory results showed bacterial infections in 25 patients (89.2%); Pseudomonas was the most common organism (13/25; 52%). Acanthamoeba, herpes simplex virus, and Aspergillus niger were isolated in one case each. Laboratory-based medical therapy led to the healing of ulcers in 24 (85.7%) of 28 patients, whereas 4 patients required penetrating keratoplasty. Most of the organisms were sensitive to antibiotics.
  
  
  CONCLUSIONS
  Contact lens-associated microbial keratitis is rare in southern India. Soft contact lens wear is the most common risk factor; Pseudomonas keratitis is the predominant causative agent. Fungal, viral, and Acanthamoeba keratitis are rare. The offending bacteria are usually sensitive to antibiotics, and the treatment outcome with medical therapy is good.
authors.affiliation: Jhaveri Microbiology Centre, Prof. Brien Holden Eye Research Centre, Hyderabad Eye Research Foundation, L. V. Prasad Eye Institute, Hyderabad, India. savitri@lvpeye.stph.net
authors.forename: Savitri
authors.initials: S
authors.lastname: Sharma
authors.forename: Sujatha
authors.initials: S
authors.lastname: Gopalakrishnan
authors.forename: Murali K
authors.initials: MK
authors.lastname: Aasuri
authors.forename: Prashant
authors.initials: P
authors.lastname: Garg
authors.forename: Gullapalli N
authors.initials: GN
authors.lastname: Rao
mesh_terms: D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000627:therapeutic use; D001419:Bacteria / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D003131:Combined Modality Therapy; D003261:Contact Lenses / Q000009:adverse effects*; D003315:Cornea / Q000382:microbiology; D015817:Eye Infections / Q000453:epidemiology / Q000209:etiology*; D005260:Female; D005658:Fungi / Q000302:isolation & purification; D018259:Herpesvirus 1, Human / Q000302:isolation & purification; D006801:Humans; D015994:Incidence; D007194:India / Q000453:epidemiology; D007223:Infant; D007634:Keratitis / Q000453:epidemiology* / Q000382:microbiology*; D015948:Keratoplasty, Penetrating; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents