Egg-matrix for large-scale single-step affinity purification of plant lectins with different carbohydrate specificities.
Hen eggs represent an easily available and inexpensive source of glycoproteins expressing a variety of sugars. Egg glycoproteins might therefore be exploited to purify by affinity chromatography carbohydrate-binding proteins (lectins) with different specificities. A method to generate an affinity matrix from hen eggs is described. The matrix was assayed for its ability to purify in a single step biologically active phytohemagglutinin, wheat germ agglutinin, lentil lectin, and peanut agglutinin. Milligrams of purified lectins per gram of matrix was obtained, with the only exception of peanut agglutinin that was not efficiently retained into the affinity column. Hen egg chromatography is a relatively simple, fast, and reproducible method to purify high amount of plant lectins.
Zoccatelli
Gianni
G
Dipartimento Scientifico e Tecnologico, Facoltà di Scienze MM.FF.NN., Università di Verona, Strada Le Grazie 15, CV1, I-37134 Verona, Italy.
Dalla Pellegrina
Chiara
C
Vincenzi
Simone
S
Rizzi
Corrado
C
Chignola
Roberto
R
Peruffo
Angelo D B
AD
D000818:Animals; D050260:Carbohydrate Metabolism; D002645:Chickens; D002846:Chromatography, Affinity / Q000379:methods*; D004531:Eggs; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000737:chemistry; D006420:Hemeproteins / Q000302:isolation & purification / Q000378:metabolism; D019887:Peanut Agglutinin / Q000302:isolation & purification / Q000378:metabolism; D037121:Plant Lectins / Q000302:isolation & purification* / Q000378:metabolism*; D013379:Substrate Specificity; D014908:Triticum / Q000737:chemistry; D014909:Wheat Germ Agglutinins / Q000302:isolation & purification / Q000378:metabolism
D006420:Hemeproteins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D014909:Wheat Germ Agglutinins; C033619:lentil lectin