Induction of anti-actin drug resistance in Tetrahymena.
Both cytochalasin D and latrunculin B reversibly inhibited Tetrahymena phagocytosis at concentrations similar to those effective in mammalian systems, even though ciliate actins are known to be highly divergent from mammalian actins. Overnight exposure to relatively low (0.25 microM) concentrations of latrunculin B induced resistance in Tetrahymena to the inhibitory effects of that drug, as well as cross-resistance to cytochalasin D. However, much higher (> 30 microM) concentrations of cytochalasin D were required for induction of cross-resistance to latrunculin B. Anti-actin drug resistance in Tetrahymena may involve a general multidrug resistance mechanism and/or specific feedback regulation of F-actin assembly and stability.
Zackroff
Robert V
RV
Massachusetts College of Pharmacy and Health Sciences, School of Arts and Sciences, 179 Longwood Avenue, Boston, MA 02115 USA. rzackroff@mcp.edu
Hufnagel
Linda A
LA
D000199:Actins / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology*; D015638:Cytochalasin D / Q000494:pharmacology*; D018432:Drug Resistance, Multiple; D021261:Parasitic Sensitivity Tests; D010587:Phagocytosis / Q000187:drug effects; D013768:Tetrahymena / Q000187:drug effects* / Q000378:metabolism; D013844:Thiazoles / Q000494:pharmacology*; D053778:Thiazolidines
D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D013844:Thiazoles; D053778:Thiazolidines; D015638:Cytochalasin D; C037068:latrunculin B