Ultrastructural observation of spermatozoa and fertilization in Schistosoma japonicum.
The ultrastructure of the sperm and the process of fertilization are described in Schistosoma japonicum. The sperm of S. japonicum has an elongated head and a single tail. The head measures 6.2 x 1.4 microm in average size. No acrosome is present. A mass of mitochondria locates in front of the nucleus. A layer of about 100-120 peripheral microtubules is parallel with the long axis of the head under plasma membrane. The nucleus is dense with some electron-lucent patches. The tail is a single flagellum with unique axoneme, which originates from a centriole. The structure of axoneme includes two types: 9 x 2 + <<l>> in the main part of the flagellum, and 9 x 2 + 0 near the end of the flagellum. The sperm ultrastructure of S. japonicum is similar to that of other schistosomes, apart from the fact that two types of configuration coexisted in the same axoneme, and there is no striated root found in S. japonicum. The sperm differs distinctly from other Digenea. The aberrant ultrastructure of S. japonicum reflects that its evolution is far away from other genera in Digenea. Fertilization occurs at the posterior part of oviduct, in which region the oviduct wall lacks lamellae. Some cortical granules (CG) fuse with plasma membrane, and discharge their content on the surface of the fertilized ovum. The other CGs break down or degenerate in the cytoplasm. By the secondary mature division, the secondary oocyte finally divides to form a female pronucleus. During this period a male pronucleus also forms. The female and male pronucleus approach each other, come into contact in the central region and finally fuse to form a zygote. The function of CGs is discussed.
Yang
Ming-Yi
MY
Research Laboratory of Schistosomiasis, Department of Medical Parasitology, Medical School, Wuhan University, 430071 Wuhan, People's Republic of China.
Dong
Hui-Fen
HF
Jiang
Ming-Sen
MS
D000818:Animals; D005260:Female; D005306:Fertilization / Q000502:physiology*; D008297:Male; D008854:Microscopy, Electron; D008870:Microtubules / Q000648:ultrastructure; D010063:Ovum / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D012549:Schistosoma japonicum / Q000196:embryology* / Q000648:ultrastructure; D013077:Sperm Head / Q000648:ultrastructure; D013082:Sperm Tail / Q000648:ultrastructure; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000166:cytology / Q000648:ultrastructure*