title: Heart-selective expression of the chicken FK506-binding protein (FKBP) 12.6 gene during embryonic development.
abstract: FKBP12.6, a member of the family of FK506-binding proteins, selectively associates with the cardiac isoform of the ryanodine receptor and thereby stabilizes this Ca(2+) release channel. A chicken FKBP12.6 (chFKBP12.6) cDNA was cloned and shown to encode a protein of 108 amino acids. The deduced amino acid sequence of chFKBP12.6 is 91-92% identical to those of mammalian FKBP12.6 proteins. Northern blot analysis revealed that chFKBP12.6 mRNA is largely restricted to the heart during embryonic development and that the abundance of this mRNA in the heart decreases, and it becomes restricted to the atrium during cardiogenesis. In situ hybridization revealed that chFKBP12.6 mRNA is localized to the precardiac mesoderm before formation of the primitive heart tube. Expression of the chFKBP12.6 gene was initially apparent throughout the developing multichambered heart but became restricted to the atria before hatching. Reverse transcription and polymerase chain reaction analysis demonstrated that chFKBP12.6 mRNA is present in the embryo from early gastrulation and is most abundant immediately after the onset of the heartbeat. These observations suggest that the chFKBP12.6 gene is expressed before heart morphogenesis to play a role in excitation-contraction coupling in cardiomyocytes and that the function of the encoded protein becomes increasingly restricted to the atrium during embryonic development.
authors.affiliation: United Graduate School of Agricultural Science, Gifu University, Gifu, Japan.
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Yazawa
authors.forename: Koji
authors.initials: K
authors.lastname: Obata
authors.forename: Akio
authors.initials: A
authors.lastname: Iio
authors.forename: Masafumi
authors.initials: M
authors.lastname: Koide
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yokota
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Sasaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kagami
authors.forename: Tamao
authors.initials: T
authors.lastname: Ono
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002642:Chick Embryo; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology*; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009206:Myocardium / Q000166:cytology*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D022021:Tacrolimus Binding Proteins / Q000096:biosynthesis*; D013997:Time Factors
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D022021:Tacrolimus Binding Proteins