title: Characterization of Staphylococcus aureus strains isolated from bovine mastitis in Japan.
abstract: Fifty-three strains of Staphylococcus aureus isolated from cows affected with mastitis from 21 prefectures in Japan were characterized by phenotypic and genotypic methods. Thirty-three (62.3%) strains showed biotype K-beta+CV:A, coagulase type VI, and sensitivity to bovine phages of group III or IV. These 33 strains could be subdivided into two groups on the basis of the production of staphylococcal enterotoxins (SEs) and on toxic shock syndrome toxin-1 (TSST-1). By pulsed-field gel electrophoresis, the 16 SEC- and TSST-1-producing strains showed similar patterns that differed by only a few fragments, suggesting that they were genetically closely related. Fifteen of 17 non SEC-producing strains which did not produce any other SEs and TSST-1 were genetically different from the SEC-producing strains and showed genetic diversity.
authors.affiliation: Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University, Kobe-shi, Hyogo, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Nagase
authors.forename: Akira
authors.initials: A
authors.lastname: Shimizu
authors.forename: Junichi
authors.initials: J
authors.lastname: Kawano
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamashita
authors.forename: Haruo
authors.initials: H
authors.lastname: Yoshimura
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Ishimaru
authors.forename: Akemi
authors.initials: A
authors.lastname: Kojima
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle; D005260:Female; D007564:Japan / Q000453:epidemiology; D008414:Mastitis, Bovine / Q000453:epidemiology / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology* / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*