Cell-type-specific adhesion onto polymer surfaces from mixed cell populations.
The targeted adhesion of a specific cell type from a mixed cell suspension via the surface presentation of a cell-specific ligand is demonstrated. This generic strategy is illustrated by the covalent attachment of a galactose derivative to a polylysine backbone via the amine functionality. Following adsorption of the resultant material to a polymer surface, hepatocyte adhesion is increased via the interaction between galactose and asialoglycoprotein receptors in a concentration-dependent manner. The selective nature of the material is demonstrated by the approximate doubling in the adhesion of hepatocytes relative to a nontargeted cell type (hepatic stellate cells), and an inability of the modified polymer surface to attract additional numbers of the nontargeted cells. This strategy provides a mechanism for controlling the ratios of cell types adhering to scaffold supports, thus enabling the rapid creation of defined coculture systems from heterogeneous cell suspensions.
Quirk
Robin A
RA
School of Pharmaceutical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom.
Kellam
Barrie
B
Bhandari
Rena N
RN
Davies
Martyn C
MC
Tendler
Saul J B
SJ
Shakesheff
Kevin M
KM
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002469:Cell Separation / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D018920:Coculture Techniques / Q000379:methods*; D005690:Galactose / Q000737:chemistry / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects / Q000502:physiology*; D007728:Kupffer Cells / Q000187:drug effects / Q000502:physiology; D019344:Lactic Acid / Q000737:chemistry; D008567:Membranes, Artificial; D011091:Polyesters; D011107:Polylysine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D051381:Rats; D013045:Species Specificity; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D011107:Polylysine; D019344:Lactic Acid; C033616:poly(lactide); D005690:Galactose