Roles of cell-wall invertases and monosaccharide transporters in the growth and development of Arabidopsis.
The hydrolysis of sucrose by cell-wall invertases (cwINV) and the subsequent import of hexoses into target cells appears to be crucial for appropriate metabolism, growth and differentiation in plants. Hexose uptake from the apoplast is catalysed by monosaccharide/H+ symporters (Sugar Transport Proteins or STPs), which have the potential to sense sugars. Import of extracellular hexoses may generate signals to orchestrate cellular activities, or simply feed metabolic pathways distinct from those fed by sucrose. It is predicted that Arabidopsis has six cwINV genes and at least 14 STP genes. These genes show different spatial and temporal patterns of expression, and several knock-out mutants have been isolated for analysis. AtSTP1 transports glucose, galactose, xylose, and mannose, but not fructose. It accounts for the majority of the AtSTP activity in vegetative tissues and its activity is markedly repressed by treatment with exogenous sugars. These observations are consistent with a role in the retrieval of cell-wall-derived sugars, for example, during carbohydrate limitation or cell expansion. The AtSTP1 gene is also expressed in developing seeds, where it might be responsible for the uptake of glucose derived from imported sucrose. The large number of AtcwINV and AtSTP genes, together with complex patterns of expression for each, and the possibility that each protein may have more than one physiological function, provides the plant with the potential for a multiplicity of patterns of monosaccharide utilization to direct growth and differentiation or to respond flexibly to changing environmental conditions.
Sherson
Sarah M
SM
Institute of Cell and Molecular Biology, University of Edinburgh, The King's Buildings, Mayfield Road, Edinburgh EH9 3JH, Scotland, UK.
Alford
Heather L
HL
Forbes
Susan M
SM
Wallace
Graham
G
Smith
Steven M
SM
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D002473:Cell Wall / Q000201:enzymology*; D003600:Cytosol / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D006601:Hexoses / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D028081:Proton-Phosphate Symporters / Q000235:genetics / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism; D043324:beta-Fructofuranosidase
D029681:Arabidopsis Proteins; D002241:Carbohydrates; D006601:Hexoses; D009004:Monosaccharide Transport Proteins; D028081:Proton-Phosphate Symporters; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase