title: Yellow fever virus replicons as an expression system for hepatitis C virus structural proteins.
abstract: Chimeric yellow fever virus (YF) RNAs were constructed in which the YF structural genes were replaced by the hepatitis C virus (HCV) structural genes or fusions between the YF and HCV structural genes. Interestingly, RNA replication required nucleotide complementarity between the 3'-located conserved sequence 1 and an RNA sequence located in the 5' end of the YF capsid sequence. The (chimeric-)HCV structural proteins were efficiently expressed and processed, and the native E1/E2 heterodimer was formed. However, no indication for the production of HCV-like particles was obtained.
authors.affiliation: Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, The Netherlands.
authors.forename: Richard
authors.initials: R
authors.lastname: Molenkamp
authors.forename: Engbert A
authors.initials: EA
authors.lastname: Kooi
authors.forename: Marjoleine A
authors.initials: MA
authors.lastname: Lucassen
authors.forename: Sophie
authors.initials: S
authors.lastname: Greve
authors.forename: Joyphi C P
authors.initials: JC
authors.lastname: Thijssen
authors.forename: Willy J M
authors.initials: WJ
authors.lastname: Spaan
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Bredenbeek
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005814:Genes, Viral; D016174:Hepacivirus / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D012093:Replicon; D015678:Viral Structural Proteins / Q000235:genetics*; D015005:Yellow fever virus / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D015678:Viral Structural Proteins