Galactosylated chitosan (GC)-graft-poly(vinyl pyrrolidone) (PVP) as hepatocyte-targeting DNA carrier. Preparation and physicochemical characterization of GC-graft-PVP/DNA complex (1).
Galactosylated chitosan was conjugated with poly(vinyl pyrrolidone) (PVP) as a hydrophilic group. The complex formation of GC-graft-PVP (GCPVP)/DNA complexes was confirmed by agarose gel electrophoresis. The morphology of the complex observed by atomic force microscopy had a compact and spherical shape, around 40 nm particle sizes at a charge ratio of 3. The binding strength of GCPVP 10K/DNA complex measured by ethidium bromide binding assay was superior to that of the GCPVP 50K/DNA one, probably attributable to its higher flexibility due to the smaller size, whereas the DNase I protection of GCPVP 10K/DNA complex was inferior to that of the GCPVP 50K/DNA one. This indicated that effective complex formation required both higher binding strength and minimal molecular weight of polycation enough to induce the condensation of DNA. The DNA-binding property of GCPVP mainly depended on the molecular weight of chitosan and composition of PVP.
Park
I K
IK
School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, South Korea.
Ihm
J E
JE
Park
Y H
YH
Choi
Y J
YJ
Kim
S I
SI
Kim
W J
WJ
Akaike
T
T
Cho
C S
CS
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002686:Chitin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry*; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D022781:Hepatocytes / Q000187:drug effects*; D008297:Male; D011205:Povidone / Q000008:administration & dosage / Q000737:chemistry*; D012474:Salmon
D004337:Drug Carriers; D002686:Chitin; D004247:DNA; D048271:Chitosan; D011205:Povidone