High-performance thin-layer chromatography-bioautography for multiple antibiotic residues in cow's milk.
An analytical method to identify and quantify multiple antibiotic residues (chloramphenicol, ampicillin, benzylpenicillin, dicloxacillin and erythromycin) in cow's milk by high-performance thin-layer chromatography (HPTLC) combined with bioautography was developed. The test microorganism used for bioautography was Bacillus subtilis ATCC 6633. Antibiotic residues were extracted with acetonitrile, fat eliminated with petroleum ether and residues isolated with dichloromethane The sensitivity of the method guarantees the detection of the above-mentioned antibiotics at levels below maximum residue limits (MRL) allowed for milk. Percentage recoveries ranged between 90 and 100%, with coefficients of variation between 7.2 and 21.3%. Some advantages of this methodology over thin-layer chromatography (TLC)/bioautography are also discussed.
Ramirez
Acacia
A
Departamento de Producción Agricola y Animal, Universidad Autónoma Metropolitana, Unidad Xochimilco, Calzada del Hueso No. 1100, Col. Villa Quietud, 04960, D.F., Coyoacán, Mexico. acaram@cueyatl.uam.mx
Gutiérrez
Rey
R
Diaz
Gilberto
G
González
Clementina
C
Pérez
Norma
N
Vega
Salvador
S
Noa
Mario
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D002855:Chromatography, Thin Layer / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D008892:Milk / Q000737:chemistry*
D000900:Anti-Bacterial Agents