title: [Application of less-than-one-hour in situ hybridization to diagnosis of infectious agents].
abstract: Less-than-one-hour in situ hybridization manual method for immunocytochemical analyses of formalin-fixed, paraffin embedded tissue sections was used to detect infectious agents. The method employs capillary action to sequentially apply, incubate and remove liquid reagents from opposite pairs of glass microscope slides and allows for simultaneous immunocytochemical analyses. We used this method to detect EB virus in nasopharyngeal tissues and human papilloma virus in cervical tissues. This rapid and sensitive procedure represents a significant improvement for clinical immunocytochemical and research laboratories alike.
authors.affiliation: Department of Ololaryngology, Shenzhen City People's Hospital, Shenzhen 518020.
authors.forename: M
authors.initials: M
authors.lastname: Liu
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: Q
authors.initials: Q
authors.lastname: Pang
authors.forename: C
authors.initials: C
authors.lastname: Liang
authors.forename: C S
authors.initials: CS
authors.lastname: Park
mesh_terms: D020031:Epstein-Barr Virus Infections; D005260:Female; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D009303:Nasopharyngeal Neoplasms / Q000821:virology*; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections; D014412:Tumor Virus Infections; D002583:Uterine Cervical Neoplasms / Q000821:virology*