title: Expression of bcl(xs) and c-myc in atretic follicles of mouse ovary.
abstract: At birth, in the human female, one million follicles are present, out of which only 450 ovulate during the reproductive lifespan while the remainder of the 99.9% follicles degenerate. It is therefore important to understand the regulation of follicular atresia. Immature mice injected with pregnant mare serum gonadotrophin (PMSG) were used as a model for follicular atresia. In this model, we demonstrated by various methods, including the TdT-mediated dUTP nick end labelling (TUNEL) technique, that granulosa cells in atretic follicles undergo apoptosis. In eukaryotic cells, apoptosis is regulated by genes such as bcl(2) and c-myc. Regulation of apoptosis in the ovary by these proteins/genes was studied using the mouse model. bcl(2) is anti-apoptotic; however, bcl(xs), a member of the bcl(2) family, is apoptotic. Immunohistochemical localization of bcl(xs) in the granulosa cells of atretic follicles suggested its role in follicular atresia. Expression of c-myc was studied by in-situ hybridization in the mouse follicular atresia model. In the atretic follicles, expression of c-myc in granulosa cells was observed. Thus our studies indicate that bcl(xs) regulates granulosa cell apoptosis, while c-myc may also play a role in cell death during follicular atresia.
authors.affiliation: Department of Cell Biology, Institute for Research in Reproduction, Parel, Mumbai 400012, India.
authors.forename: Tarala D
authors.initials: TD
authors.lastname: Nandedkar
authors.forename: Shalmali J
authors.initials: SJ
authors.lastname: Dharma