Spinning-disk confocal microscopy -- a cutting-edge tool for imaging of membrane traffic.
Confocal laser scanning microscopy is experiencing a revolution in speed from the world of seconds to that of milliseconds. The spinning Nipkow disk method with microlenses has made this remarkable innovation possible. In combination with the ultrahigh-sensitivity, high-speed and high-resolution camera system based on avalanche multiplication of photoconduction, we are now able to observe the extremely dynamic movement of small vesicles in living cells in real time.
Nakano
Akihiko
A
Molecular Membrane Biology Laboratory, RIKEN Discovery Research Institute, Saitama, Japan. nakano@postman.riken.go.jp
D000818:Animals; D005057:Eukaryotic Cells / Q000166:cytology* / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods / Q000639:trends; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure*; D007909:Lenses / Q000592:standards / Q000639:trends; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods / Q000639:trends*; D018715:Microscopy, Video / Q000295:instrumentation / Q000379:methods / Q000639:trends; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure*; D022161:Transport Vesicles / Q000378:metabolism / Q000648:ultrastructure*