[An analysis of hepatitis G virus infection status in various cases of hepatitis in Guangdong area].
To understand hepatitis G virus(HGV) infection status in different clinical types of hepatitis, 721 cases with hepatitis were analyzed in this article. PT-PCR technique was applied to amplify HGV gene, and heat denaturation method was used to extract HCV-RNA from sera. After turning to cDNA by reverse transcription(PT), a nested amplification was conducted and a 238 bp specific fragment was obtained. In this way, serum specimens of 721 cases with hepatitis, were tested including 587 hospitalized cases. 80 cases were found to be HGV RNA positive, of which positive rate of patients with non A-E hepatitis was high, HCV and HBV + HCV came the second, chronic severe hepatitis and liver cirrhosis (LC) was the lower. Results of research showed that HGV infection is prevalent to a certain degree among patients with viral hepatitis in Guangdong area.
Yao
C
C
Department of Infectious Diseases, Third Affiliated Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou 510630.
Zhou
Y
Y
Xie
D
D
D018178:Flaviviridae Infections / Q000821:virology*; D029407:GB virus C / Q000235:genetics / Q000302:isolation & purification*; D006525:Hepatitis, Viral, Human / Q000821:virology*; D006801:Humans; D012367:RNA, Viral / Q000097:blood*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015163:Superinfection / Q000821:virology
D012367:RNA, Viral