title: Functional role of N-linked glycosylation on the rat melanin-concentrating hormone receptor 1.
abstract: Melanin-concentrating hormone (MCH) is known to act through two G-protein-coupled receptors MCHR1 and MCHR2. MCHR1 has three potential sites (Asn13, Asn16 and Asn23) for N-linked glycosylation in its extracellular amino-terminus which may modulate its reactivity. Site-directed mutagenesis of the rat MCHR1 cDNA at single or multiple combinations of the three potential glycosylation sites was used to examine the role of the putative carbohydrate chains on receptor activity. It was found that all three potential N-linked glycosylation sites in MCHR1 were glycosylated, and that N-linked glycosylation of Asn23 was necessary for full activity. Furthermore, disruption of all three glycosylation sites impaired proper expression at the cell surface and receptor activity. These data outline the importance of the N-linked glycosylation of the MCHR1.
authors.affiliation: Department of Pharmacology, Saitama Medical School, 38 Moro-Hongo, Moroyama-cho, Iruma-gun, 350-0495, Saitama, Japan. yumisait@saitama-med.ac.jp
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Saito
authors.forename: Mitsue
authors.initials: M
authors.lastname: Tetsuka
authors.forename: Li
authors.initials: L
authors.lastname: Yue
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Kawamura
authors.forename: Kei
authors.initials: K
authors.lastname: Maruyama
mesh_terms: D000818:Animals; D001216:Asparagine / Q000737:chemistry; D001665:Binding Sites; D020013:Calcium Signaling; D002460:Cell Line; D006031:Glycosylation; D006801:Humans; D008024:Ligands; D016297:Mutagenesis, Site-Directed; D051381:Rats; D011979:Receptors, Pituitary Hormone / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D008024:Ligands; D011979:Receptors, Pituitary Hormone; D011994:Recombinant Proteins; C092274:melanin-concentrating hormone receptor; D001216:Asparagine