Rapid diagnosis of invasive pulmonary aspergillosis by quantitative polymerase chain reaction using bronchial lavage fluid.
Polymerase chain reaction (PCR) is a sensitive method for detection of Aspergillus DNA in bronchoalveolar lavage fluid, but it has not yet been able to distinguish infection from contamination. We have established a technique to quantify Aspergillus DNA using a real-time PCR method to resolve this problem, and we report herein a successful application of real-time PCR to diagnose invasive pulmonary aspergillosis by comparing the amount of Aspergillus DNA in bronchial lavage fluid from an affected area to that from an unaffected area. This novel tool will provide rapid, sensitive, and specific diagnosis of pulmonary aspergillosis.
Kawazu
Masahito
M
Department of Hematology & Oncology, University of Tokyo, Tokyo, Japan.
Kanda
Yoshinobu
Y
Goyama
Susumu
S
Takeshita
Masataka
M
Nannya
Yasuhito
Y
Niino
Miyuki
M
Komeno
Yukiko
Y
Nakamoto
Tetsuya
T
Kurokawa
Mineo
M
Tsujino
Shiho
S
Ogawa
Seishi
S
Aoki
Katsunori
K
Chiba
Shigeru
S
Motokura
Toru
T
Ohishi
Nobuya
N
Hirai
Hisamaru
H
D000328:Adult; D001228:Aspergillosis / Q000175:diagnosis* / Q000209:etiology / Q000235:genetics / Q000473:pathology; D001232:Aspergillus fumigatus / Q000235:genetics / Q000302:isolation & purification*; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D003199:Computer Systems; D018549:Cryptogenic Organizing Pneumonia / Q000209:etiology; D004271:DNA, Fungal / Q000032:analysis*; D005260:Female; D006086:Graft vs Host Disease / Q000209:etiology; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects / Q000627:therapeutic use; D015470:Leukemia, Myeloid, Acute / Q000150:complications; D008172:Lung Diseases, Fungal / Q000175:diagnosis* / Q000209:etiology / Q000235:genetics / Q000473:pathology; D009894:Opportunistic Infections / Q000175:diagnosis / Q000209:etiology / Q000235:genetics / Q000473:pathology; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014184:Transplantation, Homologous
D004271:DNA, Fungal; D007166:Immunosuppressive Agents