title: Maintaining GFP tissue fluorescence through bone decalcification and long-term storage.
authors.affiliation: Pennsylvania State University College of Medicine, Hershey, PA, USA.
authors.forename: John F
authors.initials: JF
authors.lastname: Harms
authors.forename: Lynn R
authors.initials: LR
authors.lastname: Budgeon
authors.forename: Neil D
authors.initials: ND
authors.lastname: Christensen
authors.forename: Danny R
authors.initials: DR
authors.lastname: Welch
mesh_terms: D000143:Acids / Q000494:pharmacology; D000230:Adenocarcinoma / Q000737:chemistry / Q000556:secondary*; D000818:Animals; D001859:Bone Neoplasms / Q000737:chemistry / Q000556:secondary*; D001842:Bone and Bones / Q000737:chemistry* / Q000187:drug effects; D001943:Breast Neoplasms / Q000473:pathology*; D003648:Decalcification Technique; D004492:Edetic Acid / Q000494:pharmacology*; D005260:Female; D005404:Fixatives / Q000494:pharmacology*; D005453:Fluorescence; D005629:Frozen Sections; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008164:Luminescent Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011309:Preservation, Biological; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000737:chemistry; D016707:Tissue Fixation / Q000379:methods*; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000637:transplantation
chemical_list: D000143:Acids; D005404:Fixatives; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D004492:Edetic Acid