title: The spoilage yeast Zygosaccharomyces bailii forms mitotic spores: a screening method for haploidization.
abstract: Zygosaccharomyces bailii ISA 1307 and the type strain of this spoilage yeast show a diploid DNA content. Together with a rather peculiar life cycle in which mitotic but no meiotic spores appear to be formed, the diploid DNA content explains the observed difficulties in obtaining auxotrophic mutants. Mitotic chromosome loss induced by benomyl and selection on canavanine media resulted in three haploid strains of Z. bailii. This new set of Z. bailii strains allows the easy isolation of recessive mutants and is suitable for further molecular genetic studies.
authors.affiliation: Instituto de Ciências da Vida e Saúde and Centro de Ciências do Ambiente, Departamento de Biologia, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Fernando
authors.initials: F
authors.lastname: Rodrigues
authors.forename: Paula
authors.initials: P
authors.lastname: Ludovico
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Sousa
authors.forename: H Yde
authors.initials: HY
authors.lastname: Steensma
authors.forename: Manuela
authors.initials: M
authors.lastname: Côrte-Real
authors.forename: Cecília
authors.initials: C
authors.lastname: Leão
mesh_terms: D001542:Benomyl / Q000494:pharmacology; D003470:Culture Media; D004271:DNA, Fungal / Q000235:genetics; D006238:Haploidy; D008938:Mitosis; D009154:Mutation; D009172:Mycology / Q000379:methods; D013172:Spores, Fungal / Q000502:physiology; D020068:Zygosaccharomyces / Q000235:genetics* / Q000254:growth & development*
chemical_list: D003470:Culture Media; D004271:DNA, Fungal; D001542:Benomyl