title: Local administration of growth hormone enhances periimplant bone reaction in an osteoporotic rabbit model.
abstract: OBJECTIVE
  The purpose of this study was to determine whether rhGH administered locally during the surgical placement of an implant, in the tibia of an osteoporotic rabbit model, would induce qualitative and quantitative differences in peri-implant bone reaction.
  
  
  METHODS
  Eight New Zealand rabbits were ovariectomized and fed with a low-calcium diet (with 0.07% of calcium) to induce osteoporosis. After 6 weeks, an experimental titanium sheet was inserted into the rabbit tibiae. The rabbits were randomly divided into two groups. The experimental group was treated with 4 IU of rhGH added locally into the ostectomy as a lyophilized powder and the control group was left without any treatment. After 2 weeks, animals were sacrificed, tibial sections were prepared and bone-titanium interface was examined at light microscopy, using Masson, hematoxylin-eosin and Pichrosirius stains. Light microscopic morphometry and densitometry were used to comparatively quantitate bone reaction.
  
  
  CONCLUSIONS
  Local administration of rhGH during the surgical placement of titanium sheets on the tibiae of an osteoporotic rabbit model enhances periosteal and transcortical reaction and mineralization of osteoid 14 days later around titanium sheets, without increasing bone resorption.
authors.affiliation: Department of Oral Medicine and Bucofacial Surgery, School of Dentistry, Complutense University, Madrid, Spain. f3f@eresmas.com
authors.forename: Isabel F
authors.initials: IF
authors.lastname: Tresguerres
authors.forename: Celia
authors.initials: C
authors.lastname: Clemente
authors.forename: Manuel
authors.initials: M
authors.lastname: Donado
authors.forename: Luis
authors.initials: L
authors.lastname: Gómez-Pellico
authors.forename: Luis
authors.initials: L
authors.lastname: Blanco
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Alobera
authors.forename: Jesús A F
authors.initials: JA
authors.lastname: Tresguerres
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001391:Azo Compounds; D015519:Bone Density / Q000502:physiology; D001857:Bone Matrix / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects* / Q000503:physiopathology / Q000601:surgery; D002113:Calcification, Physiologic / Q000187:drug effects; D004396:Coloring Agents; D003720:Densitometry; D015921:Dental Implants; D004195:Disease Models, Animal; D005260:Female; D005456:Fluorescent Dyes; D013006:Growth Hormone / Q000008:administration & dosage / Q000627:therapeutic use*; D016348:Osseointegration; D010024:Osteoporosis / Q000503:physiopathology*; D010027:Osteotomy; D010052:Ovariectomy; D010521:Periosteum / Q000187:drug effects; D011817:Rabbits; D011897:Random Allocation; D011994:Recombinant Proteins; D013223:Statistics as Topic; D013499:Surface Properties; D013977:Tibia / Q000601:surgery; D014025:Titanium / Q000737:chemistry
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D015921:Dental Implants; D005456:Fluorescent Dyes; D011994:Recombinant Proteins; C009798:C.I. direct red 80; D013006:Growth Hormone; D014025:Titanium