title: A novel monoclonal antibody 107-1A4 with high prostate specificity: generation, characterization of antigen expression, and targeting of human prostate cancer xenografts.
abstract: Monoclonal antibodies with high specificity for prostate tissue are of interest for prostate cancer research and treatment. Reactivity and specificity of a new murine monoclonal antibody, 107-1A4, was assessed by immunohistochemistry, ELISA and indirect immunofluorescence (IDIF). 107-1A4 stained all normal and malignant prostate tissue specimens while reactivity to non-prostate tissue was limited to the tubules of the normal kidney and renal cell carcinoma. Twenty two human cell lines were included in the reactivity survey; only the immunogen prostate cancer line LNCaP reacted with 107-1A4. Seminal plasma proteins PSA, PAP, PSMA, and PSP-94 were determined not to be the 107-1A4 antigen.
authors.affiliation: Department of Urology, University of Washington, Seattle, WA 98195, USA.
authors.forename: L G
authors.initials: LG
authors.lastname: Brown
authors.forename: S K
authors.initials: SK
authors.lastname: Wegner
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: K R
authors.initials: KR
authors.lastname: Buhler
authors.forename: E W
authors.initials: EW
authors.lastname: Arfman
authors.forename: P H
authors.initials: PH
authors.lastname: Lange
authors.forename: R L
authors.initials: RL
authors.lastname: Vessella