title: [Study on the origin of aberrant clones with chromosome abnormalities in MDS].
abstract: OBJECTIVE
  To study the origin of aberrant clones with chromosome abnormalities in MDS.
  
  
  METHODS
  Antibodies recognizing myeloid cells (CD33), B cells (CD19) and T cells (CD2) were used to sort bone marrow cells of ten MDS patients with chromosome abnormalities (trisomy 8 five cases, trisomy 8 accompanying trisomy 9 one case, trisomy 8 accompanying 7p- one case, 20q- two cases, t (1; 7) one case) by Immuno-Magnetic Bead technique, then the positive cells for CD33, CD19 or CD2 were detected respectively by interphase FISH using the centromeric probes for chromosomes 1, 7and 8, and the locus specific probe for 20q12. 200 to 300 cells were scored for each detection.
  
  
  RESULTS
  FISH showed that trisomy 8, t (1; 7) and 20q- all affected CD33 positive cells, but not CD2 positive cells in these ten MDS patients. Only in one MDS patient, 20q- affected the CD19 positive cells.
  
  
  CONCLUSIONS
  Our data suggest that the chromosomal aberrations in MDS are mostly restricted to myeloid cell lineages, but B lymphocytes are involved in minor MDS implying that abnormal clone originated from a multipotent hematopoietic progenitor.
authors.affiliation: First affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Suzhou, Jiangsu, 215006 China.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Shen
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Xue
authors.forename: Jianyong
authors.initials: J
authors.lastname: Li
authors.forename: Jinlan
authors.initials: J
authors.lastname: Pan
authors.forename: Suning
authors.initials: S
authors.lastname: Chen
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000276:immunology; D018941:Antigens, CD19 / Q000276:immunology; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018801:CD2 Antigens / Q000276:immunology; D002869:Chromosome Aberrations; D002999:Clone Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D063268:Sialic Acid Binding Ig-like Lectin 3
chemical_list: D015703:Antigens, CD; D018941:Antigens, CD19; D015214:Antigens, Differentiation, Myelomonocytic; D018801:CD2 Antigens; C571163:CD33 protein, human; D063268:Sialic Acid Binding Ig-like Lectin 3