title: Synergistic anticancer action of reversibly and irreversibly acting ligands of poly (ADP-ribose) polymerase.
abstract: The synergistic interaction of two ligands (INH2BP and the prodrug INO2BA) of PARP I has been demonstrated for two human leukemia cell lines (855-2 and HL-60), for a human lung cancer cell (A549) and for Eras 20 cancer cells. Synergism was calculated using kinetic combination constants based on cell multiplication rates. Reducing cellular GSH content by BSO strongly augmented synergism, an effect partly explained by the removal of C-NO scavenging (by GSH). However, INH2BP action was augmented by BSO, an effect most probably explained by the sensitization of the cell to apoptosis by GSH removal.
authors.affiliation: Department of Anatomy, School of Medicine, University of California, San Francisco Medical Center, San Francisco, CA 94143-0452, USA. ernestkun@mac.com
authors.forename: Ernest
authors.initials: E
authors.lastname: Kun
authors.forename: Eva
authors.initials: E
authors.lastname: Kirsten
authors.forename: Jerome
authors.initials: J
authors.lastname: Mendeleyev
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism*; D004357:Drug Synergism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D008024:Ligands; D011065:Poly(ADP-ribose) Polymerases; D005978:Glutathione