LytG of Bacillus subtilis is a novel peptidoglycan hydrolase: the major active glucosaminidase.
LytG (YubE) of Bacillus subtilis is a novel 32 kDa autolysin produced during vegetative growth under the control of Esigma(A) RNA polymerase. Muropeptide analysis of vegetative cells of B. subtilis revealed LytG to be the major glucosaminidase responsible for peptidoglycan structural determination during vegetative growth. Overexpression and purification of LytG allowed its biochemical characterization. Despite sequence homology suggesting muramidase activity, LytG is a novel glucosaminidase with exoenzyme activity and may form part of a novel family of autolysins. It is involved in cell division, lysis, and motility on swarm plates.
Horsburgh
Gavin J
GJ
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, United Kingdom.
Atrih
Abdelmadjid
A
Williamson
Michael P
MP
Foster
Simon J
SJ
D001412:Bacillus subtilis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001426:Bacterial Proteins / Q000737:chemistry* / Q000502:physiology; D001433:Bacteriolysis / Q000502:physiology; D001483:Base Sequence; D002455:Cell Division / Q000502:physiology; D002473:Cell Wall / Q000201:enzymology / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D010450:Endopeptidases / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D006596:Hexosaminidases / Q000737:chemistry* / Q000502:physiology; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009068:Movement / Q000502:physiology; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000032:analysis; D010457:Peptidoglycan / Q000737:chemistry; D011485:Protein Binding
D001426:Bacterial Proteins; D010455:Peptides; D010457:Peptidoglycan; D006596:Hexosaminidases; D010450:Endopeptidases; C019404:gamma-D-glutamyl-meso-diaminopimelate peptidase I; D009238:N-Acetylmuramoyl-L-alanine Amidase