title: Levels of soluble CD27 in sera and synovial fluid and its expression on memory T cells in patients with juvenile idiopathic arthritides.
abstract: OBJECTIVE
  CD27 is a member of tumour necrosis factor receptor family. Its expression is predominantly confined to mature lymphocytes and is strongly enhanced after cell activation. Shedding of the CD27 from the surface of activated cells is related to their effector phase. The aim of the present study was to evaluate the levels of soluble CD27 in sera and synovial fluids, together with its expression on circulating and synovial fluid (SF) memory T cells, in children with JIA.
  
  
  METHODS
  Sera from 40 patients with active JIA were studied for soluble CD27. Paired SF samples were available in 20 patients. Sera from 12 age-matched patients affected with various acute infectious diseases and 12 age-matched healthy subjects were used as controls. In 8 JIA patients freshly isolated circulating and SF lymphocytes were stained for CD27 in CD4+CD45 RO+ T cell subpopulation and analyzed by cytometry.
  
  
  RESULTS
  Soluble CD27 serum levels were significantly higher in patients with polyarticular JIA and acute systemic infectious diseases than in patients with active oligoarticular or healthy controls. Both polyarticular and oligoarticular JIA patients showed increased levels of soluble CD27 in SF when compared with paired serum samples (p = 0.01). In all the patients tested a significant enrichment of CD27- T cells was seen in the SF (median 39.5%, range 18-56%) when compared to paired CD4+CD45RO+ peripheral lymphocytes (median 19.5%, range 5-43%; p = 0.01).
  
  
  CONCLUSIONS
  A clear enrichment of CD4+ memory SF T cells with a CD27-phenotype is observed when compared to correspondent circulating T lymphocytes. This issue is conceivably related to re-activation and recruitment of memory T cells to the site of inflammation, and to the subsequent expansion of a subpopulation of "effector" memory T cells.
authors.affiliation: 2nd Department of Pediatrics, G. Gaslini Scientific Institute for Children and University of Genoa, Largo G. Gaslini 5, 16147, Genoa, Italy. marcogattorno@ospedale-gaslini.ge.it
authors.forename: M
authors.initials: M
authors.lastname: Gattorno
authors.forename: I
authors.initials: I
authors.lastname: Prigione
authors.forename: S
authors.initials: S
authors.lastname: Vignola
authors.forename: F
authors.initials: F
authors.lastname: Falcini
authors.forename: S
authors.initials: S
authors.lastname: Chiesa
authors.forename: F
authors.initials: F
authors.lastname: Morandi
authors.forename: P
authors.initials: P
authors.lastname: Picco
authors.forename: A
authors.initials: A
authors.lastname: Buoncompagni
authors.forename: A
authors.initials: A
authors.lastname: Martini
authors.forename: V
authors.initials: V
authors.lastname: Pistoia
mesh_terms: D000293:Adolescent; D001171:Arthritis, Juvenile / Q000097:blood* / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005434:Flow Cytometry; D006801:Humans; D007156:Immunologic Memory; D007596:Joints / Q000473:pathology; D013582:Synovial Fluid / Q000166:cytology / Q000378:metabolism*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000097:blood*
chemical_list: D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7