title: Cytokine profile of a Holstein calf with bovine leukocyte adhesion deficiency during the acute-phase inflammatory response.
abstract: Changes in interleukin (IL)-1beta, IL-6 and IL-8 in serum, and their mRNA expression on neutrophils from a 4.6-month old Holstein young calf with bovine leukocyte adhesion deficiency (BLAD) during the acute phase were evaluated. IL-1beta concentrations in the serum of the calf with BLAD at age 143-162 days ranged from 8.7 to 16.6 ng/ml, whereas the values were less than 2.7 ng/ml in control calves. Serum IL-6 (0.04 ng/ml) was only detected on the 1st day when the animal was diagnosed with the BLAD. IL-1beta and IL-8 mRNA expression on neutrophils from the affected calf appeared to be similar to those of controls. Serum cytokine levels and their mRNA expression on neutrophils from the calf with BLAD appeared to be little affected by the deficient expression of beta(2)-integrin on leukocytes, and are considered to be modulated by the inflammatory stimuli.
authors.affiliation: Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Nagahata
authors.forename: Katsuro
authors.initials: K
authors.lastname: Hagiwara
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kasamatsu
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Higuchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kurosawa
mesh_terms: D000210:Acute-Phase Reaction / Q000276:immunology*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000235:genetics / Q000276:immunology; D005786:Gene Expression Regulation; D030342:Genetic Diseases, Inborn / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000662:veterinary; D007375:Interleukin-1 / Q000097:blood / Q000235:genetics; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics; D016209:Interleukin-8 / Q000097:blood / Q000235:genetics; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000097:blood / Q000235:genetics / Q000276:immunology* / Q000662:veterinary*; D009504:Neutrophils / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D012333:RNA, Messenger