[Effects of alpha-tocopherol and beta-carotene on the oxidized low density lipoprotein induced by Cu2+].
The effects of different concentrations of alpha-tocopherol and beta-carotene on low density lipoprotein(LDL) oxidation were examined. LDL was oxidized by Cu2+ in a cell-free(10 mumol/L Cu2+ in PBS) system. The results showed that alpha-tocopherol and beta-carotene inhibited the oxidative modification of LDL in this system as evidenced by a concentration-dependent decrease in lipid peroxide(thiobarbituric-acid-reasting substances activity, TBARS) and the electrophoretic mobility of LDL. The effect of alpha-tocopherol on the generation of TBARS was stronger, while on the electrophoretic mobility was weaker than that of beta-carotene. Both alpha-tocopherol and beta-carotene slightly but not significantly reduced lipofusin. It suggested that the mechanisms were different. Thus, beta-carotene, as well as alpha-tocopherol, inhibit LDL oxidation and might play important roles in reducing the generation of oxidized LDL.
Cheng
X
X
Department of Biochemistry, Shanxi Medical University, Taiyuan 030001, China.
Cui
Y
Y
Chen
Y
Y
Zhang
X
X
D000975:Antioxidants / Q000494:pharmacology*; D003300:Copper / Q000032:analysis; D008077:Lipoproteins, LDL / Q000032:analysis*; D010084:Oxidation-Reduction; D024502:alpha-Tocopherol / Q000494:pharmacology*; D019207:beta Carotene / Q000494:pharmacology*
D000975:Antioxidants; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D019207:beta Carotene; D003300:Copper; D024502:alpha-Tocopherol