[Comparison of ITS sequences between wild ginseng DNA and garden ginseng DNA].
OBJECTIVE
To compare the genetic differences between wild ginseng and garden ginseng (Panax ginseng).


METHODS
The sequences of ITS1 and ITS2 of wild ginsengs were determined on LKB DNA sequencing station through Si-liver Sequence DNA Sequencing System. The sequencies were aligned with DNA SIS software.


CONCLUSIONS
The ITS1 and ITS2 of Panax were 220-221 and 222-224 bases in length respectively. In Panax ginsehg, the seqences of ITS1 were very stable, but ITS2 were changeable. The ITS2 sequences of No. 87 and No. 110 of the wild ginseng collected from Fusong Heilongjiang (China) were exactly the same as those of No. U41680(Jun Wen) and No. U41682(Jun Wen) of garden ginseng collected from Heilongjiang Province (China) and Korea respectively, but different from those of No. U41681(Jun Wen) from Hubei Province (China) in three bases (447, 449, 450) The result implies that the cultivated ginsengs may have been introduced from two different populations of the wild ginseng.
Ma
X J
XJ
Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100094, China.
Wang
X Q
XQ
Xiao
P G
PG
Hong
D Y
DY
D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D008969:Molecular Sequence Data; D005894:Panax / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D020506:Untranslated Regions
D018744:DNA, Plant; D004275:DNA, Ribosomal; D020506:Untranslated Regions