Hyperinsulinemia and oxidative stress.
The aim of the study was to compare the effect of short-term hyperglycemia and short-term hyperinsulinemia on parameters of oxidative stress in Wistar rats. Twenty male rats (aged 3 months, average weight 325 g) were tested by hyperinsulinemic clamp (100 IU/l) at two different glycemia levels (6 and 12 mmol/l). Further 20 rats were used as a control group infused with normal saline (instead of insulin) and 30 % glucose simultaneously. Measured parameters of oxidative stress were malondialdehyd (MDA), reduced glutathion (GSH) and total antioxidant capacity (AOC). AOC remained unchanged during hyperglycemia and hyperinsulinemia. Malondialdehyde (as a marker of lipid peroxidation) decreased significantly (p<0.05) during the euglycemic hyperinsulinemic clamp, and increased significantly during isolated hyperglycemia without hyperinsulinemia. Reduced glutathion decreased significantly (p<0.05) during hyperglycemia without hyperinsulinemia. These results suggest that the short-term exogenous hyperinsulinemia reduced the production of reactive oxygen species (ROS) during hyperglycemia in an animal model compared with the control group.
Kyselová
P
P
Department of Internal Medicine, University Hospital, Pilsen, Czech Republic.
Zourek
M
M
Rusavý
Z
Z
Trefil
L
L
Racek
J
J
D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D005978:Glutathione / Q000097:blood; D006943:Hyperglycemia / Q000378:metabolism*; D006946:Hyperinsulinism / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000502:physiology*; D010865:Pilot Projects; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D013997:Time Factors
D000975:Antioxidants; D008315:Malondialdehyde; D005978:Glutathione