Pseudomonas type III effector AvrPtoB induces plant disease susceptibility by inhibition of host programmed cell death.
The AvrPtoB type III effector protein is conserved among diverse genera of plant pathogens suggesting it plays an important role in pathogenesis. Here we report that Pseudomonas AvrPtoB acts inside the plant cell to inhibit programmed cell death (PCD) initiated by the Pto and Cf9 disease resistance proteins and, remarkably, the pro-apoptotic mouse protein Bax. AvrPtoB also suppressed PCD in yeast, demonstrating that AvrPtoB functions as a cell death inhibitor across kingdoms. Using truncated AvrPtoB proteins, we identified distinct N- and C-terminal domains of AvrPtoB that are sufficient for host recognition and PCD inhibition, respectively. We also identified a novel resistance phenotype, Rsb, that is triggered by an AvrPtoB truncation disrupted in the anti-PCD domain. A Pseudomonas syringae pv. tomato DC3000 strain with a chromosomal mutation in the AvrPtoB C-terminus elicited Rsb-mediated immunity in previously susceptible tomato plants and disease was restored when full-length AvrPtoB was expressed in trans. Thus, our results indicate that a type III effector can induce plant susceptibility to bacterial infection by inhibiting host PCD.
Abramovitch
Robert B
RB
Boyce Thompson Institute for Plant Research, Department of Plant Pathology, Cornell University, Ithaca, NY 14853, USA.
Kim
Young-Jin
YJ
Chen
Shaorong
S
Dickman
Martin B
MB
Martin
Gregory B
GB
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000633:toxicity*; D020022:Genetic Predisposition to Disease; D007113:Immunity, Innate; D051379:Mice; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000166:cytology / Q000382:microbiology / Q000502:physiology; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000502:physiology; D019253:Proto-Oncogene Proteins c-bcl-2; D011549:Pseudomonas / Q000472:pathogenicity*; D012231:Rhizobium / Q000235:genetics; D017384:Sequence Deletion; D014026:Nicotiana / Q000166:cytology / Q000382:microbiology* / Q000502:physiology; D051028:bcl-2-Associated X Protein
D001426:Bacterial Proteins; C494827:Bax protein, mouse; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; C081695:avrPto protein, Pseudomonas syringae; D051028:bcl-2-Associated X Protein