title: Characterization of the poliovirus 147S particle: new insights into poliovirus uncoating.
abstract: A Sabin 1 strain poliovirus (PV) mutant, S1(2Y-1I), carrying a Tyr at amino acid position VP2(142) and an Ile at position VP1(160), can establish persistent infections in HEp-2c cells. This mutant forms atypical 147S particles upon interaction at 0 degrees C with either cells expressing PV receptor (PVR) CD155, or PVR-IgG2a, a chimeric molecule consisting of an extracellular moiety of PVR and the hinge and Fc portion of a mouse IgG2a. Upon interaction with PVR at 37 degrees C, S1(2Y-1I), similar to the parental strain, forms both 135S A particles and 80S empty capsids. At 0 degrees C, surprisingly, at a concentration equal to or greater than 5 nM, PVR-IgG2a induced both the extrusion of VP4 from the capsid of S1(2Y-1I) and the formation of 80S particles. The same transitions were observed at 0 degrees C with the parental strain Sabin 1 at 40 nM PVR-IgG2a. Thus, the formation of 80S particles and VP4 extrusion, considered as one of the steps of PV uncoating, can be temperature-independent at high PVR concentration. This implies that structural changes of the PV capsid occurred following adsorption at low temperature.
authors.affiliation: Unité de Neurovirologie et Régénération du Système Nerveux, Institut Pasteur, 25 rue du Dr Roux, 75724, Paris Cedex, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Pelletier
authors.forename: Laurent
authors.initials: L
authors.lastname: Ouzilou
authors.forename: Minetaro
authors.initials: M
authors.lastname: Arita
authors.forename: Akio
authors.initials: A
authors.lastname: Nomoto
authors.forename: Florence
authors.initials: F
authors.lastname: Colbère-Garapin
mesh_terms: D002213:Capsid / Q000737:chemistry*; D036022:Capsid Proteins / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008565:Membrane Proteins; D017955:Poliovirus / Q000737:chemistry* / Q000502:physiology; D011487:Protein Conformation; D011991:Receptors, Virus / Q000502:physiology; D014771:Virion / Q000737:chemistry* / Q000502:physiology
chemical_list: D036022:Capsid Proteins; D007074:Immunoglobulin G; D008565:Membrane Proteins; D011991:Receptors, Virus; C066100:poliovirus receptor