Expression of autotaxin (NPP-2) is closely linked to invasiveness of breast cancer cells.
Autotaxin (ATX), originally isolated from human melanoma cells, is a novel metastasis-enhancing motogen and angiogenesis factor. In the present study, we compared the expression level of ATX mRNA between normal and breast cancer tissues and found that the expression of ATX mRNA was closely linked to invasiveness of cancer cells. Reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemical analysis showed higher cellular ATX mRNA expression in the cancer than normal breast tissues. MDA-MB-435S breast cancer cells, expressing higher amount of ATX mRNA, showed greater relative invasiveness to fibroblast-conditioned medium (FCM) than MCF7, MDA-MB-231, and HBL-100 breast cancer cells. Furthermore, ATX-transfected MCF7 cells showed increased motility and invasiveness than vector-transfected MCF7 cells. Collectively, our results suggest that the expression of ATX is closely linked to the invasiveness of breast cancer cells.
Yang
So Young
SY
College of Medicine, Konyang University, Nonsan, Korea.
Lee
Jangsoon
J
Park
Chang Gyo
CG
Kim
Seonghwan
S
Hong
Sungyoul
S
Chung
Hyun Cheol
HC
Min
Seong Ki
SK
Han
Jeung Whan
JW
Lee
Hyang Woo
HW
Lee
Hoi Young
HY
D000328:Adult; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D015197:Carcinogenicity Tests; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology*; D017077:Culture Media, Conditioned; D005260:Female; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics / Q000378:metabolism*; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D009097:Multienzyme Complexes; D009361:Neoplasm Invasiveness; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D012016:Reference Values; D014162:Transfection; D014407:Tumor Cells, Cultured; D000099189:Lysophospholipase D
D017077:Culture Media, Conditioned; D005956:Glucose-6-Phosphate Isomerase; D006023:Glycoproteins; D009097:Multienzyme Complexes; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D000099189:Lysophospholipase D