Effect of media supplements and culture conditions on inulinase production by an actinomycete strain.
Streptomyces sp. GNDU 1 produced high levels of extra-cellular inulinase (0.552 IU/ml) after 24 h at pH 7.5, temperature 46 degrees C in the presence of 1% inulin. The optimum temperature and pH for enzyme activity were 60 degrees C and 5.5 respectively. Yeast extract as a nitrogen source was found to be most suitable one for inulinase production whereas ammonium ion was inhibitory to the enzymatic production. All these conditions make Streptomyces sp. GNDU 1, a potential candidate for industrial enzymatic production of fructose from inulin.
Gill
Prabhjot Kaur
PK
Department of Biotechnology, Guru Nanak Dev University, Amritsar 143005, Punjab, India. pjk_gill@yahoo.com
Sharma
Arun Dev
AD
Harchand
Rajesh Kumari
RK
Singh
Prabhjeet
P
D003470:Culture Media; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007221:Industry; D013302:Streptomyces / Q000201:enzymology*; D013696:Temperature
D003470:Culture Media; D006026:Glycoside Hydrolases; C023086:inulinase