Induction of heat shock protein 40 and GrpE mRNAs following transient focal cerebral ischemia in the rat.
Cerebral ischemia is associated with the induction of several heat shock proteins (HSPs), but the effects on HSP40 and GrpE are less clear. The present study investigated the induction of Hsp40 and GrpE mRNAs following 30 min of middle cerebral artery occlusion in the rat model. Reverse transcription-polymerase chain reaction (PCR) and in situ hybridization analyses showed significant induction of both mRNAs in the ischemic cortex. These results demonstrate the synergic induction of HSP70 molecular chaperone machinery in cerebral ischemia.
Tamura
Shin'ya
S
Department of Neurosurgery, Akita University School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan.
Kinouchi
Hiroyuki
H
Izaki
Kenji
K
Okubo
Atsuya
A
Sugawara
Taku
T
Kunizuka
Hisanori
H
Mizoi
Kazuo
K
D000818:Animals; D001345:Autoradiography; D002540:Cerebral Cortex; D017931:DNA Primers; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D017403:In Situ Hybridization; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D020768:Middle Cerebral Artery / Q000502:physiology; D018832:Molecular Chaperones / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; C109172:GrpE-like 1 mitochondrial protein, vertebrate; C484655:Grpel1 protein, rat; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D012333:RNA, Messenger