[PCR amplification and cloning of virulence expression regulatory gene toxR of Vibrio cholerae].
In order to construct a genomic bivalent oral vaccine of Leishmania donovani and Vibrio cholerae, we amplified a 1.3 kb DNA fragment from 7 strains of Vibrio cholerae with primers P1 and P2. Restriction endonuclease analysis of PCR amplified products from 9 strains of Vibrio cholerae was performed by digestion with EcoR I. The results revealed an EcoR I site in the central part of toxR gene. The entire toxR gene of Vibrio cholerae Non-CT strain 7743 was amplified by PCR with primers P1 and P2, digested with endonucleases BamH I and Hind III, then was orientationally cloned into plasmid pAT153. The restriction endonuclease analysis demonstrates that the recombinant plasmid ptR4 contains a 1.3 kb toxR gene fragment.
Chen
J
J
Department of Parasitology, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
Ma
Y
Y
Yang
S
S
Wang
Y
Y
D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D005784:Gene Amplification; D010544:Peroxidases; D016133:Polymerase Chain Reaction; D014734:Vibrio cholerae / Q000235:genetics*
D001426:Bacterial Proteins; D010544:Peroxidases; C089974:TagD protein, Vibrio cholerae