[Monoclonal anti-NSE-antibodies: purification, characterization, and immunoenzyme analysis].
The results of NSE purification procedure, as well as hybridoma technology of anti NSE monoclonal antibodies synthesis are presented. The employment of this procedure yielded highly purified NSE preparation. The immunization of BALB/C mice with NSE preparation led to sensitization of the immunocompetent cells, which could form hybridomes, producing the anti-NSE monoclonal antibodies, after the confluence with myeloma cells Sp 2/0-Ag 14. The ELISA test-system for NSE analysis was developed on the basis of highly purified NSE preparation and monoclonal anti-NSE antibodies. This system was characterized by high specificity, accuracy and reliability. This system may be recommended for analysis of blood-brain barrier functions in the neurological and psychiatric diseases.
Chekhonin
V P
VP
Serbsky National Research Centre for Social and Forensic Psychiatry, Moscow, Russia.
Gurina
O I
OI
Portnaia
T S
TS
Riabukhin
I A
IA
Shepeleva
I I
II
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D001921:Brain / Q000201:enzymology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D010751:Phosphopyruvate Hydratase / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D010751:Phosphopyruvate Hydratase