Evaluation of skin barrier function using direct current II: effects of duty cycle, waveform, frequency and mode.
The aim of this study was to evaluate the reduction in skin barrier function caused by pulsed iontophoresis by measuring resistance in the short term. Experiments under direct current (DC) and pulsed direct current (PDC) conditions were carried out using rat abdominal skin in vivo. The resistance was measured every 62.5 micros and analyzed using a two-compartment model consisting of surface and skin resistance. Moreover, the initial value and the rate constant of surface resistance were calculated with the non-linear approximation program. Using this method, effects of duty cycle, waveform, frequency and mode on the skin barrier function were examined. The barrier function decreased with increasing duty cycle. With regard to waveforms, the influence on the skin barrier function was greatest in the order of sine, rectangular, saw and triangular waveforms. A frequency of less than 100 Hz reduced the barrier function. Considering the reduction in barrier function and skin polarization, the PDC of a rectangular waveform (around 75% as duty cycle and less than 100 Hz as frequency) or sine waveform was most suitable. In addition, the difference in the amount of drug delivered by DC and by PDC was discussed.
Kanebako
Makoto
M
Fuji Research Laboratories, Pharmaceutical Division, Kowa Company, LTD, Shizuoka, Japan. m-kanebk@kowa.co.jp
Inagi
Toshio
T
Takayama
Kozo
K
D000279:Administration, Cutaneous; D000818:Animals; D004553:Electric Conductivity; D017097:Electric Impedance; D004566:Electrodes; D005712:Galvanic Skin Response / Q000502:physiology; D007478:Iontophoresis / Q000295:instrumentation / Q000379:methods*; D009991:Oscillometry / Q000295:instrumentation / Q000379:methods; D051381:Rats; D012869:Skin Absorption / Q000502:physiology*