Protein reorientation and bound water molecules measured by 1H magnetic spin-lattice relaxation.
The water-proton spin-lattice relaxation rate constant, 1/T(1), was measured as a function of magnetic field strength for several dilute protein solutions. By separating the intermolecular contributions from the intramolecular contributions to the water-proton spin-lattice relaxation, the number of water molecules that bind to the protein for a time long compared with the rotational correlation time may be measured. We find a good correlation between the number of long-lived water molecules and the predictions based on available free volume in the proteins studied. The rotational correlation times of these proteins are larger than predicted by the Stokes-Einstein-Debye (SED) model for a sphere reorienting in a viscous liquid. The discrepancy between experiment and theory is usually attributed to hydration effects increasing the effective radius of the particle. However, the average lifetime of water molecules at the protein interface is far too short to justify such a picture. We suggest that surface roughness may be responsible for the retardation of rotational mobility and find that the SED model provides a reasonable representation of experiment if the radius assumed for the reorienting particle is the arithmetic mean of the crystallographic packing radius and the radius deduced from the effective surface area of the protein.
Van-Quynh
Alexandra
A
Chemistry Department, University of Virginia, Charlottesville 22904-4319, USA.
Willson
Steven
S
Bryant
Robert G
RG
D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000737:chemistry; D003574:Cytochrome c Group / Q000737:chemistry; D017666:Deuterium Oxide / Q000737:chemistry; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009038:Motion; D010179:Pancreas / Q000378:metabolism; D010434:Pepsin A / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D011522:Protons; D012259:Ribonuclease, Pancreatic / Q000737:chemistry; D012399:Rotation; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012996:Solutions / Q000737:chemistry; D013113:Spin Labels; D019097:Spin Trapping; D013820:Thermolysin / Q000737:chemistry; D014867:Water / Q000737:chemistry*
D003574:Cytochrome c Group; D046911:Macromolecular Substances; D011506:Proteins; D011522:Protons; D012996:Solutions; D013113:Spin Labels; D014867:Water; D012710:Serum Albumin, Bovine; D012259:Ribonuclease, Pancreatic; D002918:Chymotrypsin; C117608:alpha-chymotrypsin; D010434:Pepsin A; D013820:Thermolysin; D017666:Deuterium Oxide