Calreticulin-like molecule in trophozoites of Entamoeba histolytica HM1:IMSS (Swissprot: accession P83003).
In this work, we report the partial sequence of a 51 kDa protein of Entamoeba histolytica that is highly immunogenic in humans. Partial sequencing of the N-terminal end showed that 18 of the first 20 amino acid residues of the protein were identified uniquely, indicating that the final product was a homogeneous protein preparation. The N-terminal sequence that was found was: KVYFEETFENGWKXIWSKW. Comparing the 19-amino acid sequence of the protein in automated databases shows significant similarity with amino acid sequences of the calreticulin-like protein of spinach leaves (77%) and of the calreticulin precursor of Dictyostelium discoideum (60%).
González
Enrique
E
Depto. de Medicina Experimental, Facultad de Medicine, UNAM, México, DF, México.
Rico
Guadalupe
G
Mendoza
Guillermo
G
Ramos
Fernando
F
García
Gabriela
G
Morán
Patricia
P
Valadez
Alicia
A
Melendro
Emma I
EI
Ximénez
Cecilia
C
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D015153:Blotting, Western; D037282:Calreticulin / Q000737:chemistry* / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004748:Entamoeba histolytica / Q000254:growth & development* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry* / Q000276:immunology*; D020539:Sequence Analysis, Protein
C470490:51 kDa protein, Entamoeba histolytica; D000913:Antibodies, Protozoan; D037282:Calreticulin; D015800:Protozoan Proteins