Very high-field EPR study of glycyl radical enzymes.
This work shows that very high-field EPR spectroscopy allows a rather accurate determination of the g-tensor of protein radicals, including C-centered ones, and thus may be used as a probe for distinguishing a tyrosyl-, a glycyl-, or a tryptophanyl-radical. In this paper, we report the first complete analysis of the g-tensor of glycyl radical enzymes (anaerobic ribonucleotide reductase, pyruvate formate lyase, and benzylsuccinate synthase), thus providing new information on their EPR properties. Because the g-anisotropy is small, the complete resolution of the g-tensor could be only obtained at very high field (18.8 T).
Duboc-Toia
Carole
C
High Magnetic Field Laboratory of Grenoble, CNRS-MPI, 25 av. des Martyrs, 38042 Grenoble, France. toia@labs.polycnrs-gre.fr
Hassan
Alia K
AK
Mulliez
Etienne
E
Ollagnier-de Choudens
Sandrine
S
Fontecave
Marc
M
Leutwein
Christina
C
Heider
Johann
J
D016880:Anisotropy; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005609:Free Radicals / Q000737:chemistry; D005998:Glycine / Q000737:chemistry*; D012264:Ribonucleotide Reductases / Q000737:chemistry*
D005609:Free Radicals; D012264:Ribonucleotide Reductases; D005998:Glycine