[Experimental study on Schwann cells cytoplasmic neurotrophic proteins to improve the regeneration of the injured peripheral nerve in vivo].
OBJECTIVE
To study the effects of Schwann cell cytoplasmic derived neurotrophic proteins (SDNF) on the regeneration of peripheral nerve in vivo.


METHODS
Ninety adult SD rats were chosen as the experimental model of degenerated muscle graft with vascular implantation bridging the 10 mm length of right sciatic nerve. They were divided randomly into three groups, 30 SD rats in each groups. 25 microliters of 26 ku SDNF (50 micrograms/ml, group A), 58 ku SDNF (50 micrograms/ml, group B) and normal saline(group C) were injected respectively into the proximal, middle and distal part of the degenerated muscle grafts at operation, 7 and 14 days postoperatively. The motorial function recovery assessment was carried out every 15 days with the sciatic nerve function index(SFI) after 15 days to 6 months of operation. Histological and electrophysiological examination of regenerating nerve were made at 1, 3 and 6 months postoperatively.


RESULTS
There were significant statistic differences between the both of experimental groups(group A and B) and control group(group C) in the respects of the histological, electrophysiological examination and SFI(P < 0.01).


CONCLUSIONS
The 26 ku SDNF and 58 ku SNDF can improve the regeneration of the injured peripheral nerve in vivo.
Xie
G
G
Department of Microsurgery, First Affiliated Hospital of Sun Yat-sen University of Medical Sciences, Guangzhou Guangdong, P. R. China 510080.
Zhu
J K
JK
Gu
X F
XF
D000818:Animals; D005260:Female; D008297:Male; D009414:Nerve Growth Factors / Q000302:isolation & purification / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects*; D016067:Nerve Transfer; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000737:chemistry*; D012584:Sciatic Nerve / Q000293:injuries* / Q000503:physiopathology* / Q000601:surgery
D009414:Nerve Growth Factors