[Anti-inflammatory effect of peroxisome proliferator-activated receptor-gamma incultured human mesangial cells].
OBJECTIVE
To investigate whether peroxisome proliferator-activator receptor-gamma (PPARgamma) could retard the inflammatory challenge from human mesangial cells.


METHODS
Based on the inflammatory mesangial cell model followed by IL-1beta, the levels of IL-6 and TNF-gamma in the supernatants were measured by enzyme-linked immunosorbant assay (ELISA). PPARgamma protein expression of human mesangial cell lines (HMCL) lysate was detected with Western blot. PPARgamma, IL-6 and TNF-gamma mRNA expression in HMCL lysate were measured by semi-quantitative RT-PCR assay.


RESULTS
The levels of IL-6 and TNF-gamma in HMCL supernatants were dramatically increased when cultured HMCL were stimulated by IL-1beta (10 ng/ml). Protein and mRNA expressions of PPARgamma in IL-1beta challenge cells were significantly increased than observed in untreated cells. However, PPARgamma agonist troglitazone down-regulated mRNA and protein expression of PPARgamma from IL-1beta challenge HMCLs. PPARgamma agonists troglitazone, rosiglitazone, and 15-deoxy-Delta(12, 14)-prosglandin J(2) significantly decreased the upexpression of TNF-alpha and IL-6 in HMCL supernatants stimulated by IL-1beta. Furthermore, troglitazone down-regulated TNF-alpha and IL-6 mRNA expression levels of HMCLs.


CONCLUSIONS
These data suggest that PPARgamma play an important role in response to inflammatory stress in mesangial cells. PPARgamma may prove to be a pharmacological target in glomerulonephritis.
Xiong
Zuying
Z
Renal Division of First Hospital, Institute of Nephrology, Peking University, Beijing 100034, China.
Huang
Haichang
H
Li
Jingzi
J
Wang
Haiyan
H
D002478:Cells, Cultured; D002839:Chromans / Q000494:pharmacology; D005920:Glomerular Mesangium / Q000166:cytology*; D005921:Glomerulonephritis / Q000209:etiology / Q000517:prevention & control*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D018160:Receptors, Cytoplasmic and Nuclear / Q000502:physiology*; D013844:Thiazoles / Q000494:pharmacology; D045162:Thiazolidinediones; D014157:Transcription Factors / Q000502:physiology*; D000077288:Troglitazone; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D002839:Chromans; D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D013844:Thiazoles; D045162:Thiazolidinediones; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha; D000077288:Troglitazone