title: [Comparison of detection of trisomy 8 with fluorescence in situ hybridization and conventional karyotype analysis in myelodysplastic syndrome].
abstract: The purpose of this study was to compare the detection of trisomy 8 in myelodysplastic syndrome (MDS) patients with interphase fluorescence in situ hybridization (FISH) and cytogenetic karyotype analysis. Using Spectrum Green labeled chromosome 8 centromere probe, interphase FISH was established. The trisomy 8 clones were simultaneously detected in 48 MDS cases with FISH and conventional cytogenetic analysis (CCA). Results showed that the CCA revealed no significant difference of constitutional proportion between MDS-RA and MDS-RAEB with karyotypes of whole +8, partial +8 and one +8. With FISH, detectable rates were 66.1% for whole +8. Partial +8 and sole +8 were significantly higher than one +8 and complex +8, respectively. The percentages of trisomy 8 were similar in MDS-RA and MDS-RAEB. Trisomy 8 was detected in 1 of 15 specimens with normal or abnormal karyotype without trisomy 8 by FISH. There was linear correlation between the percentages of partial +8 detected by FISH and CCA. Two patients received CCA and FISH examination at diagnosis and during treatment, the percentage of trisomy 8 was increased with progress of disease. In conclusion, our results showed that FISH is a sensitive and accurate technique to detect trisomy 8 in MDS patients. It can provide contribute to diagnosis, assessment of curative effect and predicting progress of disease in MDS. Clone size of trisomy 8 does not related to classification of MDS, but sole +8 is seems to see in MDS-RA frequently.
authors.affiliation: Henan Anyang People's Hospital, Anyang 455000, China.
authors.forename: Zhi-Biao
authors.initials: ZB
authors.lastname: Zhang
authors.forename: Shi-He
authors.initials: SH
authors.lastname: Liu
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Li-Jin
authors.initials: LJ
authors.lastname: Bo
authors.forename: Huan-Ying
authors.initials: HY
authors.lastname: Cui
authors.forename: Xu-Ping
authors.initials: XP
authors.lastname: Liu
authors.forename: Yan-Xia
authors.initials: YX
authors.lastname: Nie
authors.forename: Shuang
authors.initials: S
authors.lastname: Qin
mesh_terms: D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000473:pathology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014314:Trisomy