A tomato xylem sap protein represents a new family of small cysteine-rich proteins with structural similarity to lipid transfer proteins.
The coding sequence of a major xylem sap protein of tomato was identified with the aid of mass spectrometry. The protein, XSP10, represents a novel family of extracellular plant proteins with structural similarity to plant lipid transfer proteins. The XSP10 gene is constitutively expressed in roots and lower stems. The decline of XSP10 protein levels in tomato infected with a fungal vascular pathogen may reflect breakdown or modification by the pathogen.
Rep
Martijn
M
Plant Pathology, Swammerdam Institute for Life Sciences, University of Amsterdam, P.O. Box 94062, 1090 GB Amsterdam, The Netherlands. rep@science.uva.nl
Dekker
Henk L
HL
Vossen
Jack H
JH
de Boer
Albert D
AD
Houterman
Petra M
PM
de Koster
Chris G
CG
Cornelissen
Ben J C
BJ
D000595:Amino Acid Sequence; D052179:Antigens, Plant; D002352:Carrier Proteins / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry; D005670:Fusarium / Q000472:pathogenicity; D018551:Solanum lycopersicum / Q000737:chemistry* / Q000378:metabolism / Q000382:microbiology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009181:Mycoses / Q000378:metabolism; D010935:Plant Diseases; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018547:Plant Stems / Q000737:chemistry; D017434:Protein Structure, Tertiary; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D040681:Structural Homology, Protein
D052179:Antigens, Plant; D002352:Carrier Proteins; D010940:Plant Proteins; C511920:lipid transfer proteins, plant; D003545:Cysteine