title: Mitosin, a novel marker of cell proliferation and early recurrence in intracranial meningiomas.
abstract: The expression of mitosin, a novel proliferation-associated molecule was evaluated immunohistochemically in a consecutive series of 47 patients with primary intracranial benign and atypical meningiomas. Mitosin expression was correlated with proliferation markers Ki-67 (MIB-1), proliferating cell nuclear antigen (PCNA), topoisomerase IIalpha (TopoIIalpha) and mitotic index, as well as with standard clinicopathological parameters and patient outcome. Seven tumors recurred (14.8%) following gross total resection, within a follow-up period ranging from 21 to 108 months (median 60 months). The higher proliferation indices were obtained with mitosin and PCNA and the lower ones with TopoIIalpha. Mitosin labeling index (LI) ranged from 0.1 to 57% (median 3%), with a significant overlapping of values between grades. A significant positive correlation was shown between mitosin LI on the one hand and Ki-67 LI (p < 0.001), or the mitotic index (p = 0.027) on the other. The incidence of recurrence was higher in cases with a mitosin LI higher than 3% (p = 0.048). Univariate analysis disclosed mitosin LI (p = 0.033) along with the mitotic index (p = 0.024) and tumor size (p = 0.028) as significant predictors of shortened recurrence-free survival. In multivariate analysis, the labeling indices of mitosin (p = 0.035) and Ki-67 (p = 0.032), along with tumor size, were shown to provide independent prognostic information, beyond that obtained by standard clinical and pathological parameters. However, as indicated by factor analysis, the prognostic information yielded by mitosin was superior to that provided by the remaining proliferation markers (p = 0.041). We conclude that mitosin immunohistochemical expression, although failing to discriminate between benign and atypical meningiomas, may be of use as a novel cell proliferation marker and as a predictor of tumor recurrence.
authors.affiliation: Department of Pathology, Medical Faculty, National University of Athens, Greece. takonst@hol.gr
authors.forename: A E
authors.initials: AE
authors.lastname: Konstantinidou
authors.forename: P
authors.initials: P
authors.lastname: Korkolopoulou
authors.forename: N
authors.initials: N
authors.lastname: Kavantzas
authors.forename: H
authors.initials: H
authors.lastname: Mahera
authors.forename: I
authors.initials: I
authors.lastname: Thymara
authors.forename: X
authors.initials: X
authors.lastname: Kotsiakis
authors.forename: M
authors.initials: M
authors.lastname: Perdiki
authors.forename: E
authors.initials: E
authors.lastname: Patsouris
authors.forename: P
authors.initials: P
authors.lastname: Davaris
mesh_terms: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D002455:Cell Division; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004268:DNA-Binding Proteins; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008577:Meningeal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D008579:Meningioma / Q000378:metabolism*; D008840:Microfilament Proteins; D009364:Neoplasm Recurrence, Local / Q000378:metabolism* / Q000473:pathology; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D016019:Survival Analysis
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D019394:Ki-67 Antigen; D008840:Microfilament Proteins; D018809:Proliferating Cell Nuclear Antigen; C094772:centromere protein F; D004250:DNA Topoisomerases, Type II