title: Close correlation between restriction fragment length polymorphism of L-myc gene and susceptibility to gastric cancer.
abstract: OBJECTIVE
  A common inherited RFLP of the L-myc proto-oncogene has been reported to correlate with cancer susceptibility. Our aim was to test the hypothesis that there was association between L-myc S allele in gastric cancer and predisposition to the disease.
  
  
  METHODS
  The distribution of L-myc polymorphism in 25 patients with gastric cancer was determined by polymerase chain reaction-based restriction fragment length polymorphism and compared with that of 83 healthy control subjects.
  
  
  RESULTS
  We found a significant difference, both in the distribution of the LL, LS and SS genotypes and in the allelic frequencies, between the control group and the patient group; that is, the frequencies of L-myc alleles were, L and S, 0.52 and 0.48, 0.64 and 0.36, respectively. This difference was primarily the result of a high frequency of the S allele among gastric cancer patients compared to controls. There was a significant difference in the distribution of both genotypes (P = 0.004) and allele frequencies (P = 0.005) between patients with gastric cancer and control groups.
  
  
  CONCLUSIONS
  Our results suggested that L-myc polymorphism may be significant in an individual's susceptibility to gastric cancer in Turkey and may be useful for identifying patients at high risk of developing gastric cancer.
authors.affiliation: Department of Molecular Medicine, Institute of Experimental Medical Research, University of Istanbul, DETAE 34390, P.O. Box 7 Capa, Istanbul, Turkey. tisbir@superonline.com.tr
authors.forename: Turgay
authors.initials: T
authors.lastname: Isbir
authors.forename: Ilhan
authors.initials: I
authors.lastname: Yaylim
authors.forename: Soykan
authors.initials: S
authors.lastname: Arikan
authors.forename: Esra
authors.initials: E
authors.lastname: Kaytan
authors.forename: Tamer
authors.initials: T
authors.lastname: Karşidağ
authors.forename: Savaş
authors.initials: S
authors.lastname: Bayrak
authors.forename: Hakan
authors.initials: H
authors.lastname: Camlica
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D004247:DNA / Q000097:blood / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005787:Gene Frequency; D016259:Genes, myc / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D000090063:Proto-Oncogene Mas; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D017931:DNA Primers; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D004247:DNA