Local cell membrane deformations due to receptor-ligand bonding as seen by reflection microscopy.
Understanding surface receptor clustering and redistribution processes at the cell-matrix contact zone requires detailed knowledge of the spatial integration of these molecules in the architecture of this complex interface. Here we present and discuss critically a procedure to extract such information combining reflection contrast microscopy (RCM) and reflection interference microscopy (RIM). As model system, we used living human umbilical vein endothelial cells (HUVEC) adhering to laminin-coated surfaces and investigated the distribution of the alpha2beta1 (CD29/CD49b) integrin at the contact zone of these cells. First, we applied freeze-fracture electron microscopy to gain information on microscopic details of the alpha2beta1 distribution at the contact zone. Next, we visualized and analyzed the overall lateral distribution of the integrins applying RCM using immunogold-labeling with 10 nm labels and a special silver enhancement technique. We found that RCM can be used to determine the lateral position of the marked receptor molecules to an accuracy of about 100-200 nm, instead of large morphological changes at the contact zone during silver enhancement. Finally, we combined RCM with RIM and analyzed the interference pattern of the contact zone around the label positions. Thus, we were able to detect changes of the average shape of the cell membrane due to receptor-ligand bonding of a size down to the resolution of the techniques.
Galle
J
J
Institute of Anatomy, University of Leipzig, Germany.
Reibiger
I
I
Westermann
M
M
Richter
W
W
Löffler
S
S
D002448:Cell Adhesion / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000502:physiology* / Q000648:ultrastructure; D005614:Freeze Fracturing; D006801:Humans; D007124:Immunoenzyme Techniques; D038982:Integrin alpha2beta1 / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D008024:Ligands; D008857:Microscopy, Interference; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism; D014471:Umbilical Veins
D038982:Integrin alpha2beta1; D007797:Laminin; D008024:Ligands; D011956:Receptors, Cell Surface