Antibody production in early life supported by maternal lymphocyte factors.
To examine the influence of maternal lymphocyte factors on the immune responses in offspring in early life, antibody production in neonates born to either normal or lymphocyte-deficient mothers was analyzed. Recombination activating gene (Rag)-2(+/-) mouse neonates born to Rag-2(+/+), Rag-2(+/-)or Rag-2(-/-)mothers were injected with goat anti-mouse IgD antiserum, and IgE and IgG(1) production was evaluated. The levels of IgE and IgG(1) were higher in the pups born to Rag-2(+/+)and Rag-2(+/-) dams than to lymphocyte-deficient Rag-2(-/-) dams. The enhanced antibody production in the former compared with the latter neonates was also found following immunization with ovalbumin or TNP-Ficoll. Thus, the presence of maternal lymphocyte factors was suggested in neonates that augmented antigen-specific antibody production in both T cell-dependent and -independent pathways. A reduction in antibody production was observed in normal neonates when they were foster-nursed by Rag-2(-/-) mothers. Thus, the maternal lymphocyte factors enhancing the immune responses in newborns were shown to be present in breast-milk.
Shimamura
Michio
M
Laboratory of Developmental Immunology, Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Tokyo 194-8511, Machida, Japan. michio@libra.ls.m-kagaku.co.jp
Huang
Yi-Ying
YY
Goji
Hiroshi
H
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000097:blood / Q000235:genetics / Q000276:immunology*; D001942:Breast Feeding; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D005260:Female; D005362:Ficoll / Q000031:analogs & derivatives*; D007106:Immune Sera / Q000008:administration & dosage; D007072:Immunoglobulin D / Q000032:analysis; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000097:blood; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D008892:Milk / Q000276:immunology*; D010047:Ovalbumin; D014301:Trinitrobenzenes
D004268:DNA-Binding Proteins; D007106:Immune Sera; D007072:Immunoglobulin D; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C499308:Rag2 protein, mouse; C020529:TNP-ficoll; D014301:Trinitrobenzenes; C064655:V(D)J recombination activating protein 2; D005362:Ficoll; D007073:Immunoglobulin E; D010047:Ovalbumin