Tissue culture studies of retinal development.
Because of a limited number of cell types, a series of well-described cell-type-specific markers and a stereotyped sequence of cell development, the retina has been a valuable model of CNS development. Dissociated and explant cultures have been used to help define some of the requirements for differentiation of each major cell class. In addition to mixed-cell cultures it is now possible to use cell purification or selective growth methods to give cultures of single cell types. Alteration of gene expression by viral infection has proved to be a valuable method to help elucidate developmental pathways.
Zhang
Samuel Shao-Min
SS
Department of Pathology, Yale University School of Medicine, 330 Cedar Street, P.O. Box 208061, New Haven, CT 06520, USA.
Fu
Xin Yuan
XY
Barnstable
Colin J
CJ
D000818:Animals; D046508:Culture Techniques / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D018014:Gene Transfer Techniques; D012160:Retina / Q000166:cytology* / Q000254:growth & development*