The binding of cofactors to photosystem I analyzed by spectroscopic and mutagenic methods.
This review focuses on cofactor-ligand and protein-protein interactions within the photosystem I reaction center. The topics include a description of the electron transfer cofactors, the mode of binding of the cofactors to protein-bound ligands, and a description of intraprotein contacts that ultimately allow photosystem I to be assembled (in cyanobacteria) from 96 chlorophylls, 22 carotenoids, 2 phylloquinones, 3 [4Fe-4S] clusters, and 12 polypeptides. During the 15 years that have elapsed from the first report of crystals to the atomic-resolution X-ray crystal structure, cofactor-ligand interactions and protein-protein interactions were systematically being explored by spectroscopic and genetic methods. This article charts the interplay between these disciplines and assesses how good the early insights were in light of the current structure of photosystem I.
Golbeck
John H
JH
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA. JHG5@psu.edu
D001665:Binding Sites; D002734:Chlorophyll / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins; D008958:Models, Molecular; D009154:Mutation; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000235:genetics; D045331:Photosystem I Protein Complex; D011485:Protein Binding; D011487:Protein Conformation; D021122:Protein Subunits; D011506:Proteins / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics; D013057:Spectrum Analysis / Q000379:methods*; D010837:Vitamin K 1 / Q000737:chemistry*; D014961:X-Ray Diffraction / Q000379:methods
D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D021122:Protein Subunits; D011506:Proteins; D011994:Recombinant Proteins; C089909:photosystem I, psaB subunit; D002734:Chlorophyll; D010837:Vitamin K 1