Gene expression profiling: methodological challenges, results, and prospects for addiction research.
This review describes the current methods used to profile gene expression. These methods include microarrays, spotted arrays, serial analysis of gene expression (SAGE), and massive parallel signature sequencing (MPSS). Methodological and statistical problems in interpreting microarray and spotted array experiments are also discussed. Methods and formats such as minimum information about microarray experiments (MIAME) needed to share gene expression data are described. The last part of the review provides an overview of the application of gene-expression profiling technology to substance abuse research and discusses future directions.
Pollock
Jonathan D
JD
Genetics and Molecular Neurobiology Research Branch, National Institute on Drug Abuse, 6001 Executive Blvd, Rockville, MD 20850, USA. jp183r@nih.gov
D000818:Animals; D003627:Data Interpretation, Statistical; D020869:Gene Expression Profiling / Q000379:methods* / Q000592:standards; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012106:Research / Q000639:trends; D015398:Signal Transduction / Q000502:physiology; D019966:Substance-Related Disorders / Q000235:genetics* / Q000378:metabolism