title: [Determination of major components in liuyingwan pills by supercritical fluid extraction(SFE)].
abstract: OBJECTIVE
  On the basis of SFE and CGC to establish a method for the qualitative and quantitative analysis of major components in Liuyingwan pills.
  
  
  METHODS
  Orthogonal design and variance analysis were used to optimize the four variables (temperature, pressure, volume of modifier and static extraction time) of SFE, and the quantification was completed by CGC.
  
  
  RESULTS
  The optimized conditions are as follows: pressure 48.2 MPa, temperature 60 degrees C, 0.2 ml ethyl acetate as modifier and static extraction 10 min. The recoveries of borneol and eugenol are 101.93% (RSD 1.56%) and 98.43% (RSD 2.26%) respectively.
  
  
  CONCLUSIONS
  Compared with conventional methods, SFE is rapid, convenient and accurate in determining borneol and eugenol.
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: B
authors.initials: B
authors.lastname: Chen
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: H S
authors.initials: HS
authors.lastname: Fang
authors.forename: G J
authors.initials: GJ
authors.lastname: Yang
authors.forename: Y T
authors.initials: YT
authors.lastname: Wu
mesh_terms: D001892:Camphanes / Q000032:analysis; D025924:Chromatography, Supercritical Fluid; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005054:Eugenol / Q000032:analysis; D008419:Materia Medica / Q000737:chemistry*
chemical_list: D001892:Camphanes; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D008419:Materia Medica; D005054:Eugenol; C022871:isoborneol