Determination of trace isoflavone phytoestrogens in biological materials by capillary electrochromatography.
Capillary electrochromatography using a specialty monolithic matrix was utilized in developing a rapid and highly efficient separation of isoflavones in biological materials. Without a preconcentration technique, it is relatively easy to reach ppm-ppb concentrations of these compounds in soy-based foods and verify them structurally using a photodiode array detector. With on-column preconcentration, we were able to measure low-ppb levels in human serum. Using blood samples from human volunteers, whose diet was supplemented by a soy-based product, the method has been validated for high-throughput screening of isoflavones in clinical studies.
Starkey
Jason A
JA
Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
Mechref
Yehia
Y
Byun
Chang Kyu
CK
Steinmetz
Rosemary
R
Fuqua
John S
JS
Pescovitz
Ora H
OH
Novotny
Milos V
MV
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods* / Q000592:standards; D004968:Estrogens, Non-Steroidal / Q000097:blood* / Q000302:isolation & purification; D005502:Food; D006801:Humans; D007223:Infant; D007225:Infant Food; D007231:Infant, Newborn; D007529:Isoflavones / Q000097:blood* / Q000302:isolation & purification; D008895:Milk, Human / Q000737:chemistry; D048789:Phytoestrogens; D028321:Plant Preparations; D013025:Glycine max / Q000737:chemistry
D004968:Estrogens, Non-Steroidal; D007529:Isoflavones; D048789:Phytoestrogens; D028321:Plant Preparations