Immunolocalization of caveolin-1 and caveolin-3 in monkey skeletal, cardiac and uterine smooth muscles.
Caveolin, a 20-24 kDa integral membrane protein, is a principal component of caveolar domains. Caveolin-1 is expressed predominantly in endothelial cells, fibroblasts, and adipocytes, while the expression of caveolin-3 is confined to muscle cells. However, their localization in various muscles has not been well documented. Using double-immunofluorescence labeling and confocal laser microscopy, we examined the localization of caveolins-1 and 3 in adult monkey skeletal, cardiac and uterine smooth muscles and the co-immunolocalization of these caveolins with dystrophin, which is a product of the Duchenne muscular dystrophy gene. In the skeletal muscle tissue, caveolin-3 was localized along the sarcolemma except for the transverse tubules, and co-immunolocalized with dystrophin, whereas caveolin-1 was absent except in the blood vessels of the muscle tissue. In cardiac muscle cells, caveolins-1 and -3 and dystrophin were co-immunolocalized on the sarcolemma and transverse tubules. In uterine smooth muscle cells, caveolin-1, but not caveolin-3, was co-immunolocalized with dystrophin on the sarcolemma.
Hagiwara
Yasuko
Y
Department of Animal Models for Human Disease, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan. hagiwara@ncnp.go.jp
Nishina
Yasushi
Y
Yorifuji
Hiroshi
H
Kikuchi
Tateki
T
D000818:Animals; D051242:Caveolin 1; D051244:Caveolin 3; D022461:Caveolins / Q000378:metabolism*; D016189:Dystrophin / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D008252:Macaca fascicularis; D018613:Microscopy, Confocal; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D012508:Sarcolemma / Q000378:metabolism / Q000648:ultrastructure; D014599:Uterus / Q000166:cytology* / Q000378:metabolism
D051242:Caveolin 1; D051244:Caveolin 3; D022461:Caveolins; D016189:Dystrophin