Photopolymerized hyaluronic acid-based hydrogels and interpenetrating networks.
Hyaluronic acid (HA) was derivatized with methacrylic esters used for the preparation of hydrogels via photopolymerization. Poly(ethylene glycol) diacrylate (PEG-DA) with a molecular weight of 570 was also used as a comacromonomer to improve elastic modulus and swelling behavior. The hydrogels were readily degraded by hyaluronidase and their mechanical properties could be modulated by HA molecular weight and concentration of PEG-DA. The incorporation of RGD peptides allowed modulation of the HA properties from cell non-adhesive to adhesive. Human dermal fibroblasts were cultured on the RGD, RDG, and non-functionalized HA hydrogels for up to 7d, showing adhesion and proliferation only with incorporated RGD.
Park
Yong Doo
YD
Department of Materials, Institute for Biomedical Engineering, Swiss Federal Institute of Technology and University of Zürich, Moussonstrasse 18, CH-8044 Zürich, Switzerland.
Tirelli
Nicola
N
Hubbell
Jeffrey A
JA
D000595:Amino Acid Sequence; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004548:Elasticity; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D006821:Hyaluronoglucosaminidase; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry*; D007202:Indicators and Reagents; D008970:Molecular Weight; D010777:Photochemistry; D011092:Polyethylene Glycols; D012867:Skin / Q000166:cytology
D020100:Hydrogels; D007202:Indicators and Reagents; C437167:poly(ethylene glycol)diacrylate; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase