title: Increased intraabdominal pressure impairs liver regeneration after partial hepatectomy in rats.
abstract: BACKGROUND
  There are many experimental studies showing that increased intraabdominal pressure (IAP) reduces liver blood flow, leading to ischemia and portal venous congestion. But, there is no study evaluating the effect of increased IAP on liver regeneration. It is well known that acute liver ischemia and portal venous congestion impair liver regeneration. We, therefore, aimed to determine the effect of increased IAP on liver regeneration in this study.
  
  
  METHODS
  Sprague-Dawley rats underwent partial hepatectomy with or without IAP of 12-14 mm Hg for 24 h or sham operation. Rats were randomly divided into six groups: two sham-operated groups, two hepatectomy groups, and two hepatectomy with increased IAP groups. Mitotic index, proliferating cell nuclear antigen (PCNA)-labeling index, and liver regeneration rate as liver regeneration parameters were studied on day 1 or on day 4 after operation. Additionally, serum aspartate transaminase (AST) level and histopathological changes in intestinal mucosa were studied.
  
  
  RESULTS
  Hepatectomy with/without increased IAP groups had significantly higher serum AST levels than the sham-operated group on day 1. Serum AST level was found to be significantly higher in the hepatectomy with increased IAP group than in the other groups on day 4. Intestinal mucosal injury was found in the hepatectomy with increased IAP groups on days 1 and 4. Mitotic index and PCNA-labeling index were markedly higher in all hepatectomy with/without increased IAP groups than in the sham-operated groups. However, together with liver regeneration rate, both indices were significantly less in the hepatectomy with increased IAP groups than in the hepatectomy groups both on day 1 and on day 4.
  
  
  CONCLUSIONS
  Maintenance of IAP between 12 and 14 mm Hg for 24 h impaired liver regeneration after partial hepatectomy in rats.
authors.affiliation: Department of Surgery, Celal Bayer University Faculty of Medicine, 45020 Manisa, Turkey.
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Kaya
authors.forename: Erinc
authors.initials: E
authors.lastname: Aral
authors.forename: Teoman
authors.initials: T
authors.lastname: Coskun
authors.forename: Nilufer
authors.initials: N
authors.lastname: Erkasap
authors.forename: Ahmet
authors.initials: A
authors.lastname: Var
mesh_terms: D000005:Abdomen / Q000503:physiopathology*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D006498:Hepatectomy / Q000379:methods*; D007413:Intestinal Mucosa / Q000473:pathology; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D001219:Aspartate Aminotransferases