title: A simple and effective method for producing nonrandom peptide libraries using cotton as a carrier in continuous flow peptide synthesizers.
abstract: A method has been developed for generating nonrandom peptide libraries on cotton. Disks of cotton fabric were chemically modified to enable peptide synthesis. Incorporation of a 6-aminocaproic acid residue handle on the cellulose turned out to be advantageous. Disks were labeled with silver ink, stacked one on top of another in a continuous flow peptide synthesizer column, and simultaneously subjected to automated synthesis procedures. Depending on the sequences to be synthesized, the automatic synthesis procedure was stopped, and the disks were removed from the column, sorted, and reapplied to subsequent synthesis steps. In this way, individual peptides could be easily prepared in milligram quantities on each of the cotton disks.
authors.affiliation: Department of Pharmaceutical Biosciences, Division of Pharmacology, Uppsala University, Box 591, Biomedicum, SE-751 24 Uppsala, Sweden.
authors.forename: Felikss
authors.initials: F
authors.lastname: Mutulis
authors.forename: Marcus
authors.initials: M
authors.lastname: Tysk
authors.forename: Ilze
authors.initials: I
authors.lastname: Mutule
authors.forename: Jarl E S
authors.initials: JE
authors.lastname: Wikberg
mesh_terms: D001331:Automation / Q000295:instrumentation / Q000379:methods; D020650:Combinatorial Chemistry Techniques / Q000295:instrumentation / Q000379:methods; D032561:Cotton Fiber; D019151:Peptide Library; D010455:Peptides / Q000138:chemical synthesis
chemical_list: D019151:Peptide Library; D010455:Peptides