Identification of a MAP65 isoform involved in directional expansion of plant cells.
MAP65 comprises a multigene family specific to plants. To see which isoform is utilised for the unique mechanism of cell expansion, uncomplicated by division structures, carrot cells were deprived of auxin whereupon they stopped dividing and elongated instead. During elongation, a MAP65 protein triplet reduced to a single band. Mass spectrometric analysis demonstrated that this corresponded to a single carrot cDNA; it also corresponded to the major protein previously shown to form filamentous cross-bridges between microtubules in vitro. This MAP65 isoform is concluded to have a major role in establishing the parallel microtubule arrays characteristic of cells undergoing directional expansion.
Chan
Jordi
J
Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK.
Mao
Guojie
G
Smertenko
Andrei
A
Hussey
Patrick J
PJ
Naldrett
Mike
M
Bottrill
Andrew
A
Lloyd
Clive W
CW
D000595:Amino Acid Sequence; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000302:isolation & purification; D018552:Daucus carota / Q000166:cytology / Q000235:genetics; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D059828:Plant Cells; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D010944:Plants / Q000235:genetics; D020033:Protein Isoforms; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D018076:DNA, Complementary; D008869:Microtubule-Associated Proteins; D010940:Plant Proteins; D020033:Protein Isoforms