title: Historical control data on reproductive abilities and incidences of spontaneous fetal malformations in Wistar Hannover GALAS rats.
abstract: Wistar Hannover rats, which have recently been introduced into Japan, are expected to be used in reproductive and developmental toxicity studies, yet the accumulation of background data is insufficient. This paper describes our historical data on the reproductive ability of this strain of rat. Three lots of sexually matured females (40 each) were received from CLEA JAPAN, Inc. with males of the same strain (30 or 36 each) and mated. A total of 47 dams were killed on gestation day 20 to examine their fetuses. The remaining 71 pregnant females were allowed to deliver spontaneously and observed for common reproductive parameters. The mating and fertility indices of females were both 99.2%. Overall mean numbers of implants and live fetuses at cesarean sectioning were 12.5 and 11.5, respectively. Fetal resorptions and deaths occurred at an incidence of 8.6%. Morphological examinations of fetuses revealed low incidences of spontaneous malformations (each one case of double aortic arch and absent cervical vertebral arch) and a variety of common variations. The followings are overall means of major reproductive parameters obtained from females with live birth: no. of implants, 12.5; no. of pups delivered, 11.8; viability index of pups at birth, 99.8%; and days of age at sexual maturation (vaginal opening and preputial separation), 30.3 and 42.8, respectively. Our present observations confirmed a minimal deviation among 3 lots of animals in terms of reproductive abilities. These results suggest that this strain of rat can be used in reproductive and developmental toxicity studies, although the sensitivity to toxicants remains to be elucidated.
authors.affiliation: Laboratory of Reproductive Toxicology, Toxicology Division I, Institute of Environmental Toxicology, Mitsukaido, Ibaraki 303-0043, Japan. aoyama@iet.or.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Aoyama
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kikuta
authors.forename: Noriko
authors.initials: N
authors.lastname: Shirasaka
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hojo
authors.forename: Ken L
authors.initials: KL
authors.lastname: Takahashi
authors.forename: Naoko
authors.initials: N
authors.lastname: Shimizu
authors.forename: Michiko
authors.initials: M
authors.lastname: Harigae
authors.forename: Fukushi
authors.initials: F
authors.lastname: Taguchi
authors.forename: Shoji
authors.initials: S
authors.lastname: Teramoto
mesh_terms: D000818:Animals; D017849:Animals, Inbred Strains; D000013:Congenital Abnormalities / Q000453:epidemiology* / Q000235:genetics; D004971:Estrus / Q000502:physiology; D005260:Female; D005298:Fertility / Q000235:genetics / Q000502:physiology*; D014644:Genetic Variation; D008297:Male; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar