Immunomodulatory effect of glutoxim on some activities of isolated human neutrophils and in whole blood.
Activity of Glutoxim, a sulfur-containing hexapeptide with immunomodulating effect on lymphocytes, was studied on human neutrophils. Commercial available agent containing the substance, Glutoxim, was used. At the doses of 1, 3, 6 or 100 microg/mL the drug stimulated the superoxide anion and hypochlorous acid generation in resting neutrophils but inhibited them in cells stimulated with zymosan or PMA. The only inhibiting effect was observed on nitric oxide production in LPS-stimulated RAW 264.7 cells. The drug also influenced neutrophil chemotaxis showing chemoattractant activity on cells and inhibiting fMLP-induced chemotaxis. These effects on neutrophils confirm and extend range of Glutoxim immunomodulating abilities.
Fimiani
Vincenzo
V
Department of Experimental Pathology and Microbiology, Medical School, University of Messina, 98125 Messina, Italy. vincenzo.fimiani@unime.it
Cavallaro
Antonia
A
Ainis
Tommaso
T
Baranovskaia
Galina
G
Ketlinskaya
Olga
O
Kozhemyakin
Leonid
L
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000328:Adult; D000818:Animals; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006997:Hypochlorous Acid / Q000378:metabolism; D051379:Mice; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D009569:Nitric Oxide / Q000096:biosynthesis; D009842:Oligopeptides / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D009842:Oligopeptides; C462699:glutoxim; D013481:Superoxides; D009569:Nitric Oxide; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006997:Hypochlorous Acid; D013755:Tetradecanoylphorbol Acetate