Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis.
We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration.
Limatola
Cristina
C
Dipartimento di Fisiologia Umana e Farmacologia, Università di Roma La Sapienzo, Piazzale Aldo Moro 5, I00185 Rome, Italy. cristina.limatola@uniroma1.it
Di Bartolomeo
Sabrina
S
Trettel
Flavia
F
Lauro
Clotilde
C
Ciotti
Maria T
MT
Mercanti
Delio
D
Castellani
Loriana
L
Eusebi
Fabrizio
F
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D002465:Cell Movement / Q000276:immunology*; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D002525:Cerebellar Cortex / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D054360:Chemokine CXCL1; D054426:Chemokine CXCL2; D018925:Chemokines / Q000276:immunology* / Q000378:metabolism; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000276:immunology* / Q000378:metabolism; D002633:Chemotaxis / Q000276:immunology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000276:immunology* / Q000378:metabolism; D015213:Neuroimmunomodulation / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011233:Precipitin Tests; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D023063:Receptors, Interleukin-8B / Q000276:immunology* / Q000378:metabolism; D015398:Signal Transduction / Q000276:immunology
D054360:Chemokine CXCL1; D054426:Chemokine CXCL2; D018925:Chemokines; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D018691:Excitatory Amino Acid Antagonists; D036341:Intercellular Signaling Peptides and Proteins; D018091:Receptors, AMPA; D023063:Receptors, Interleukin-8B; C475829:CXCL1 protein, human; C513297:Cxcl1 protein, rat; C513296:Cxcl2 protein, rat