Comparison and validation of novel pyrogen tests based on the human fever reaction.
The Limulus amoebocyte lysate (LAL) test has replaced about 80% of the use of the rabbit pyrogen test. Ideally, human-based in vitro tests are needed, to replace the remaining use of the rabbit test and the use of the LAL test. The progress of an EU-funded project is described, in which a number of in vitro tests, based on the human fever reaction are passing through a prevalidation study on the way to evaluation in a formal validation study.
Hartung
Thomas
T
University of Konstanz, Steinbeis-Technology Transfer Center for In Vitro Pharmacology and Toxicology (STZ InPuT), 78457 Konstanz, Germany.
D000826:Animal Testing Alternatives; D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D005062:European Union; D005334:Fever / Q000139:chemically induced* / Q000503:physiopathology; D006801:Humans; D066298:In Vitro Techniques; D008033:Limulus Test; D011749:Pyrogens / Q000145:classification / Q000494:pharmacology*; D011817:Rabbits; D015203:Reproducibility of Results
D011749:Pyrogens