title: Targeted tissue oxidation in the cerebral cortex induces local prolonged depolarization and cortical spreading depression in the rat brain.
abstract: Spreading depression (SD) has been linked to several neurological disorders as epilepsy, migraine aura, trauma, and cerebral ischemia, which were also influenced by disorderliness of the brain redox homeostasis. To investigate whether local tissue oxidation directly induces SD, we oxidized a restricted local area of the rat cerebral cortex using photo-dynamic tissue oxidation (PDTO) technique and examined the cerebral blood flow (CBF) and direct current (DC) potential in and around the oxidized area. Intensive PDTO induced prolonged depolarization only in the photo-oxidized area, which led to global changes of CBF and DC potential: synchronous negative shifts of DC potential (with an amplitude of approximately 20 mV) and hyperperfusion of CBF occurred. The changes in DC potential and CBF spread at a rate of around 3mm/min beyond the oxidized area to the whole hemisphere of the cerebral cortex, indicating that intensive local oxidation induces SD in the rat brain.
authors.affiliation: Department of Neuroscience, Osaka Bioscience Institute, 6-2-4 Furuedai, Suita, Osaka 565-0874, Japan.
authors.forename: Yilong
authors.initials: Y
authors.lastname: Cui
authors.forename: Yosky
authors.initials: Y
authors.lastname: Kataoka
authors.forename: Qing Hua
authors.initials: QH
authors.lastname: Li
authors.forename: Chihiro
authors.initials: C
authors.lastname: Yokoyama
authors.forename: Aya
authors.initials: A
authors.lastname: Yamagata
authors.forename: Noriko
authors.initials: N
authors.lastname: Mochizuki-Oda
authors.forename: Jun
authors.initials: J
authors.lastname: Watanabe
authors.forename: Hisao
authors.initials: H
authors.lastname: Yamada
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000502:physiology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D013181:Cortical Spreading Depression / Q000187:drug effects / Q000502:physiology*; D005456:Fluorescent Dyes / Q000378:metabolism / Q000494:pharmacology; D008027:Light; D008297:Male; D010083:Oxidants, Photochemical / Q000378:metabolism / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D010777:Photochemistry; D051381:Rats; D017208:Rats, Wistar; D012395:Rose Bengal / Q000378:metabolism / Q000494:pharmacology
chemical_list: D005456:Fluorescent Dyes; D010083:Oxidants, Photochemical; D012395:Rose Bengal