[Early diagnosis of nasopharyngeal carcinoma using recombinant antigens expressed in bacteria].
An enzyme-linked immunosorbent assay(ELISA) was developed by using recombinant purified early antigens, EA-D and EA-R to detect IgA antibodies in sera from nasopharyngeal carcinoma(NPC) patients. 30 sera from NPC patients and 49 sera from normal persons were detected by the developed ELISA. The serum positive rate and antibody titer were compared with immunoenzymatic method(IE) on slide with Raji cells mears. ELISA is more sensitive and specific. The serum positive rate of NPC patients was increased from 70% by IE to 100% by ELISA and 77% of the serum antibody titer reached > or = 1:200. The results show that the expressed recombinant p138 and p54 antigens are effective for detection of EA/IgA antibody in sera from NPC patients and ELISA will be a sensitive, specific and convenient method for early diagnosis of NPC.
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D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004854:Herpesvirus 4, Human / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis*; D011994:Recombinant Proteins / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; C033305:Epstein-Barr virus early antigen; C083204:Epstein-Barr virus early antigen diffuse component; D007070:Immunoglobulin A; D011994:Recombinant Proteins