title: Crystallization and preliminary X-ray analysis of Alicyclobacillus acidocaldarius endoglucanase CelA.
abstract: Crystallization of a family 9 beta-1,4-glucanase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius CelA is reported. Thin plates can be obtained by hanging-drop vapour-diffusion crystallization in high concentrations (60%) of MPD. These crystals are unusual in that they do not bind the dye IZIT in the mother liquor and do not appear to dissolve in water after three weeks or in the storage buffer after 2 d. The crystals diffract weakly and the diffraction pattern is compatible with crystal disorder in one direction. After testing several crystals at the ESRF beamlines ID14-1 and ID14-2, a crystal was found which gave ordered diffraction in all directions. A full data set was collected to 3.0 A resolution, which allowed unambiguous determination of the space group as P2(1)2(1)2 and the unit-cell parameters as a = 85, b = 129.7, c = 48.6 A. Initial promising results from molecular-replacement searches are reported.
authors.affiliation: Humboldt Universität zu Berlin, Institut für Biologie, Bakterienphysiologie, Chausseestrasse 117, D-10115 Berlin, Germany.
authors.forename: Kelvin
authors.initials: K
authors.lastname: Eckert
authors.forename: Heidi A
authors.initials: HA
authors.lastname: Ernst
authors.forename: Erwin
authors.initials: E
authors.lastname: Schneider
authors.forename: Sine
authors.initials: S
authors.lastname: Larsen
authors.forename: Leila
authors.initials: L
authors.lastname: Lo Leggio
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D002480:Cellulase / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D003627:Data Interpretation, Statistical; D008958:Models, Molecular; D012984:Software
chemical_list: C074775:CelA endoglucanase; D002480:Cellulase