Identification of differentially expressed genes like cofilin2 in growing collateral arteries.
Arteriogenesis, the growth of pre-existing collateral arteries, can be induced in rabbits by occlusion of the femoral artery. In order to analyze the differential gene expression in arteriogenesis, cDNA of collateral arteries 24h after femoral occlusion or sham operation was subjected to suppression subtractive hybridization (SSH). We demonstrated an upregulation of the U6 snRNA binding protein Lsm5, cytochrome b, an expressed sequence tag, and the actin-depolymerizing factor cofilin2 mRNA in collateral arteries 24h after femoral ligation. For cofilin2, we also detected an increase in the protein level and a localization predominantly in smooth muscle cells of collaterals. Simultaneously with the upregulation of cofilin2 we found a downregulation of the alpha-smooth muscle actin mRNA in growing collateral arteries. In summary, our data showed an augmented expression level of genes contributing to different fundamental processes of arteriogenesis.
Boengler
Kerstin
K
Department of Experimental Cardiology, Max-Planck-Institute for Physiological and Clinical Research, Benekestr. 2, Bad Nauheim D-61231 , Germany. k.boengler@kerckoff.mpg.de
Pipp
Frederic
F
Broich
Kerstin
K
Fernandez
Borja
B
Schaper
Wolfgang
W
Deindl
Elisabeth
E
D051339:Actin Depolymerizing Factors; D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D001158:Arteries / Q000254:growth & development* / Q000378:metabolism*; D015152:Blotting, Northern; D051340:Cofilin 2; D003097:Collateral Circulation / Q000235:genetics / Q000502:physiology*; D020869:Gene Expression Profiling; D006614:Hindlimb / Q000098:blood supply; D008026:Ligation; D008840:Microfilament Proteins / Q000096:biosynthesis* / Q000235:genetics; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000098:blood supply / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D015854:Up-Regulation
D051339:Actin Depolymerizing Factors; D000199:Actins; D051340:Cofilin 2; D008840:Microfilament Proteins; D012333:RNA, Messenger