Regeneration of small intestinal mucosa after acute ischemia-reperfusion injury.
To determine whether c-Fos and c-Jun are involved in the repair of small intestinal mucosa after ischemia-reperfusion (I/R), we investigated the mechanism of regeneration following acute I/R injury in rats by evaluating changes in DNA synthesis, fractional synthesis rate (FSR) of proteins, and alkaline phosphatase (ALP) activity. Furthermore, we examined the sequential expression of c-Fos and c-Jun using western blot analysis and immunohistochemical staining. Proliferating cell nuclear antigen (PCNA) labeling index (LI) demonstrated that the LI of the I/R group at 2 and 6 hr after reperfusion was significantly higher than that of the controls. Statistically significant differences were found between the FSRs of the I/R group and the corresponding conventional group at 2, 6, and 12 hr. The expression of c-Fos and c-Jun proteins increased markedly after I/R and these proteins decreased with time. The levels of ALP in the I/R group were significantly decreased at 2 and 6 hr after reperfusion compared to controls. These results indicate that c-Fos and c-Jun play a central role in the repair process that results in complete restoration of intestinal mucosal function after I/R.
Itoh
Hiroshi
H
Department of Surgery (II), School of Medicine, Kanazawa University, Ishikawa 920-8641, Japan.
Yagi
Masao
M
Hasebe
Ken
K
Fushida
Sachio
S
Tani
Takashi
T
Hashimoto
Tetsuo
T
Shimizu
Kouichi
K
Miwa
Koichi
K
D000818:Animals; D015153:Blotting, Western; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000502:physiology*; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D012038:Regeneration / Q000502:physiology*; D015427:Reperfusion Injury / Q000503:physiopathology*
D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun