Gene expression profile analysis in human hepatocellular carcinoma by cDNA microarray.
We performed gene expression profiling of normal and hepatocellular carcinoma (HCC) liver tissues using a high-density microarray that contained 3,063 human cDNA. The results of a microarray hybridization experiment from eight different HCC tissues were analyzed and classified by the Cluster program. Among these differentially-expressed genes, the galectin-3, serine/threonine kinase SGK, translation factor eIF-4A, -4B, -3, fibroblast growth factor receptor, and ribosomal protein L35A were up-regulated; the mRNAs of Nip3, decorin, and the insulin-like growth factor binding protein-3 were down-regulated in HCC. The differential expression of these genes was further confirmed by an RT-PCR analysis. In addition, our data suggest that the gene expression profile of HCC varies according to the histological types.
Chung
Eun Jung
EJ
Department of Immunology, School of Medicine, Kyungpook National University, Daegu 700-422, Korea.
Sung
Young Kwan
YK
Farooq
Mohammad
M
Kim
Younghee
Y
Im
Sanguk
S
Tak
Won Young
WY
Hwang
Yoon Jin
YJ
Kim
Yang Il
YI
Han
Hyung Soo
HS
Kim
Jung-Chul
JC
Kim
Moon Kyu
MK
D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism; D016000:Cluster Analysis; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation; D015870:Gene Expression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D017931:DNA Primers; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D012333:RNA, Messenger