Comparison of UV inactivation of spores of three encephalitozoon species with that of spores of two DNA repair-deficient Bacillus subtilis biodosimetry strains.
When exposed to 254-nm UV, spores of Encephalitozoon intestinalis, Encephalitozoon cuniculi, and Encephalitozoon hellem exhibited 3.2-log reductions in viability at UV fluences of 60, 140, and 190 J/m(2), respectively, and demonstrated UV inactivation kinetics similar to those observed for endospores of DNA repair-defective mutant Bacillus subtilis strains used as biodosimetry surrogates. The results indicate that spores of Encephalitozoon spp. are readily inactivated at low UV fluences and that spores of UV-sensitive B. subtilis strains can be useful surrogates in evaluating UV reactor performance.
Marshall
Marilyn M
MM
Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA. marshalm@u.arizona.edu
Hayes
Samuel
S
Moffett
Jackie
J
Sterling
Charles R
CR
Nicholson
Wayne L
WL
D000818:Animals; D001412:Bacillus subtilis / Q000502:physiology / Q000528:radiation effects*; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D016819:Encephalitozoon / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects*; D009670:Encephalitozoon cuniculi / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D009154:Mutation; D011836:Radiation Tolerance; D013171:Spores, Bacterial / Q000528:radiation effects; D014466:Ultraviolet Rays