[Study of apoptosis in the male rat spermatogenic cells after vasostomy].
OBJECTIVE
To investigate the effect of vasostomy on apoptosis in the male rat spermatogenic cells after vasoligation.


METHODS
Model of vasoligation and vasostomy in male rat was established, and then terminal deoxynucleotidyl transferase-mediated dUTP nick labelling technique to detect the apoptosis of spermatogenic cells at 4, 8, 12, 16 weeks after vasostomy.


RESULTS
The number of apoptotic cells in vasostomy group was significantly lower than that of vasoligation group since 8 weeks after vasostomy(P < 0.05). The number of apoptotic cells in 8 and 12 weeks after vasostomy were significantly higher than that in prevasoligation(P < 0.05). 16 weeks after vasostomy, the number of apoptotic cells restored to the level same as that in prevasoligation stage.


CONCLUSIONS
Vasostomy can reverse the apoptosis of spermatogenic cells due to vasoligation.
Dong
Q
Q
Department of Urological Surgery, First University Hospital, West China University of Medical Sciences, Chengdu Sichuan, P. R. China 610041.
Yang
Y R
YR
Zhang
W D
WD
D000818:Animals; D017209:Apoptosis; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013090:Spermatocytes / Q000166:cytology*; D014669:Vasovasostomy