Protein adsorption on surfaces: dynamic contact-angle (DCA) and quartz-crystal microbalance (QCM) measurements.
Adsorption of the protein bovine serum albumin (BSA) on gold has been tested at various concentrations in aqueous solution by dynamic contact-angle analysis (DCA) and quartz-crystal microbalance (QCM) measurements. With the Wilhelmy plate technique advancing and receding contact angles and the corresponding hysteresis were measured and correlated with the hydrophilicity and the homogeneity of the surface. With electrical admittance measurements of a gold-coated piezoelectrical quartz crystal, layer mass and viscoelastic contributions to the resonator's frequency shift during adsorption could be separated. A correlation was found between the adsorbed mass and the homogeneity and hydrophilicity of the adsorbed film.
Stadler
H
H
University of Kaiserslautern, Department of Physics, Erwin Schroedinger Str. 56, 67663 Kaiserslautern, Germany.
Mondon
M
M
Ziegler
C
C
D000327:Adsorption; D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D003460:Crystallization; D006046:Gold / Q000737:chemistry*; D008962:Models, Theoretical; D011791:Quartz / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties
D011791:Quartz; D012710:Serum Albumin, Bovine; D006046:Gold