Comparison of washing and swabbing procedures for collecting genital fluids to assess shedding of human immunodeficiency virus type 1 (HIV-1) RNA in asymptomatic HIV-1-infected women.
Human immunodeficiency virus type 1 (HIV-1) RNA was detected by means of ultrasensitive reverse transcription-PCR assay of 19 cervicovaginal lavage, 21 cervical mucus, 18 vaginal wall, and 17 vaginal fornix paired samples from 25 asymptomatic HIV-1-infected women (76, 84, 72, and 68%, respectively; P > 0.5). Levels of HIV-1 RNA in cervicovaginal wash samples were highly correlated with those in paired endocervical mucus samples (r = 0.71; P = 0.0006), indicating that cervicovaginal washing and endocervical swabbing are equivalent collection procedures.
Andréoletti
Laurent
L
Laboratoire de Virologie, Centre Hospitalier Universitaire, IFR 53/EA-3309, Faculté de Médecine de Reims, Reims, France. landreoletti@chu-reims.fr
Grésenguet
Gérard
G
Chomont
Nicolas
N
Matta
Mathieu
M
Quiniou
Yann
Y
Si-Mohamed
Ali
A
Bélec
Laurent
L
D000328:Adult; D005260:Female; D005836:Genitalia, Female / Q000821:virology*; D015658:HIV Infections / Q000635:transmission / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D012367:RNA, Viral / Q000032:analysis*; D017201:Virus Shedding / Q000502:physiology*; D016387:Women's Health
D012367:RNA, Viral