title: Lincomycin resistance mutations in two regions immediately downstream of the -10 region of lmr promoter cause overexpression of a putative multidrug efflux pump in Bacillus subtilis mutants.
abstract: We isolated 19 lincomycin-resistant Bacillus subtilis mutants by expressing lmrB encoding a putative multidrug efflux protein. Eighteen of the mutants altered at two regions (-3 to -1 and +15) immediately downstream of the -10 region of the lmr promoter increased lmr transcription in vivo and in vitro.
authors.affiliation: Institute of Biological Sciences, University of Tsukuba, Tsukuba-shi, Ibaraki 305-8572, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Kumano
authors.forename: Masaya
authors.initials: M
authors.lastname: Fujita
authors.forename: Kouji
authors.initials: K
authors.lastname: Nakamura
authors.forename: Makiko
authors.initials: M
authors.lastname: Murata
authors.forename: Reiko
authors.initials: R
authors.lastname: Ohki
authors.forename: Kunio
authors.initials: K
authors.lastname: Yamane
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D008034:Lincomycin / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009154:Mutation
chemical_list: D008034:Lincomycin