Interleukin regulation of asymmetric antibody synthesized by isolated placental B cells.
OBJECTIVE
Protecting antibodies against trophoblast surface molecules were previously described. Here we analysed the synthesis of asymmetric IgG by placental B-lymphocytes.


METHODS
B cells were isolated from human term placenta and cord blood, stimulated with anti-CD40 IgG and cocultured with transfected Fcgamma R-expressing mice Ltk-fibroblast. Interleukin-4, IL-6, IL-10, IL-11 and IL-13 were added to cultures for 14 days. Asymmetric IgG were assessed in culture supernatants by concanavalin A (Con A) fixation and enzyme-linked immunosorbent assay.


RESULTS
When IL-6 was added to the cultures, the percentages of asymmetric IgG synthesized by placental B cells were: IL-6: 29 +/- 10; IL-6 + IL-10: 24 +/- 7; IL-4 + IL-10 + IL-6: 38 +/- 9. The last combination induced the highest increase in the asymmetric IgG synthesis as compared with control (19 +/- 10%, P < 0.05). Additionally, placental B cells synthesized more asymmetric IgG than umbilical cord blood B-lymphocytes (P = 0.0015).


CONCLUSIONS
Isolated placental B-lymphocytes synthesized asymmetric IgG in response to Th2 interleukins, more notably IL-6 in combination with IL-4 and IL-10. The in vitro increase of protective asymmetric IgG synthesis in response to Th2-cytokines support the hypothesis that a local Th2-switch is beneficial for pregnancy outcome.
Canellada
A
A
IDEHU-National Council of Scientific and Technological Research, University of Buenos Aires, Buenos Aires, Argentina. acanell@ffyb.uba.ar
Färber
A
A
Zenclussen
A C
AC
Gentile
T
T
Dokmetjian
J
J
Keil
A
A
Blois
S
S
Miranda
S
S
Berod
L
L
Gutiérrez
G
G
Markert
U R
UR
Margni
R A
RA
D000818:Animals; D000917:Antibody Formation / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007378:Interleukins / Q000378:metabolism*; D051379:Mice; D010920:Placenta / Q000276:immunology*
D007074:Immunoglobulin G; D007378:Interleukins