[Genetic transformation of Panax ginseng C.A. Meyer induced by root inducing plasmid(Ri) of Agrobacterium rhizogenes].
OBJECTIVE
To obtain the hairy root of Panax ginseng (HRPG).


METHODS
HRPG was obtained by infecting the germ-free seedling, cotyledon, leaf the blade, Petiole with Agrobacterium rhizogenes 15,834 harbouring agropine-type Ri plasmid. The transformation was proved by PCR and TLC.


RESULTS
HRPG grew rapidly on hormone-free medium and showed typical phenotype of hairy root. The induced rate and time can be improved by adding exogenous hormone and AS(hydroxyacetosyringone). PCR analysis confirmed the integration of TL-DNA, including 564 bp rolC sequence in the HRPG. Opine analysis evidenced the integration and expression of TL-DNA. The total saponin in HRPG (2.486%) was higher the than control(1.403% Panax ginseng).


CONCLUSIONS
HRPG grows rapidly and proves high in content of ginseng saponin. This culture system of HRPG will be useful for the production of active components in ginseng.
Liu
J
J
Department of Biotechnology, China Pharmaceutical University, Nanjing 210038, Jiangsu, China.
Ding
J Y
JY
Wang
J B
JB
D005894:Panax / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics / Q000254:growth & development; D010946:Plants, Medicinal / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics*; D012503:Saponins / Q000032:analysis; D014170:Transformation, Genetic
D012503:Saponins