title: Prevalence and HLA association of GAD65 antibodies in Hungarian schoolchildren.
abstract: Pancreas beta-cell autoantibodies are important tools in prediction of type 1 diabetes, however, background frequencies of these markers reveal considerable population-specific variations. The aim of current study was to determine the frequency of glutamic acid decarboxylase (65kD) antibodies (GADA) in healthy Hungarian schoolchildren (n = 2,664) and to correlate development of GADA with HLA DQ genotypes. GADA was determined using a radioligand assay (cut-off limit: 97.5th percentile); HLA DQ genotypes were identified with allele-specific polymerase chain reaction. Thirty-five children (1.3%) tested positive for GADA among which the proportion of girls was significantly higher when compared with boys (1.8% and 0.8%, p = 0.03). The study population was followed for 5.2 years and one girl in the whole cohort, who tested positive for GADA, has developed diabetes. HLA DQ2/DQ8 and DQ2/y genotypes (where y not equal DQB1*0302, *0301, *0602, *0603) were significantly more prevalent in the GADA positive group than in antibody negative children (17.1% vs 1.7%, p = 0.0003 and 25.7% vs 10.3%, p = 0.027, respectively). In conclusion, the prevalence of GADA in the Hungarian general population is in the middle of the range found in other Caucasian ethnic groups. Individuals carrying HLA DR3-DQ2 haplotype and females were more prone to develop GADA. We suggest that for future screening programs it is important to determine predictive value of islet-cell antibodies in populations with varying disease incidence.
authors.affiliation: Department of Pediatrics, University of Pécs, Pécs, Hungary. robert.hermann@utu.fi
authors.forename: Robert
authors.initials: R
authors.lastname: Hermann
authors.forename: Gyula
authors.initials: G
authors.lastname: Soltész
mesh_terms: D000293:Adolescent; D001323:Autoantibodies / Q000097:blood*; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D005260:Female; D005968:Glutamate Decarboxylase / Q000276:immunology*; D006683:HLA-DQ Antigens / Q000235:genetics*; D015803:HLA-DR3 Antigen / Q000235:genetics; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D007527:Isoenzymes / Q000276:immunology*; D008297:Male; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D015995:Prevalence
chemical_list: D001323:Autoantibodies; D006683:HLA-DQ Antigens; D015803:HLA-DR3 Antigen; D007527:Isoenzymes; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2