title: A 35-kDa co-aggregation factor is a hemin binding protein in Porphyromonas gingivalis.
abstract: It has been known that Porphyromonas gingivalis has an obligate requirement for hemin or selected heme- or Fe-containing compounds for its growth. In addition, the influence of hemin on the expression of several putative virulence factors produced by this bacterium has also been recently documented; however, the mechanisms involved in hemin uptake are poorly defined. We succeeded in cloning the gene coding for the 35-kDa protein, which was specifically expressed in P. gingivalis and seemed to confer colonizing activities. Recently, we have constructed the P. gingivalis 381 mutant defective in the 35-kDa protein by insertion mutagenesis. The beige mutant exhibited little co-aggregation and the virulence was also decreased. Based on these results and homology search analysis, we focused on assessing the hemin bindings and found the heme regulatory motif (HRM) as a hemin direct binding site. The 35-kDa protein did possess the binding ability of selected protoporphyrins involving the hemin. These results demonstrated that 35-kDa protein is one of the hemin binding proteins in P. gingivalis and suggested that hemin binding ability of 35-kDa protein is important for the expression of virulence in P. gingivalis.
authors.affiliation: Department of Biochemistry, Nihon University School of Dentistry at Matsudo, Chiba 271-8587, Japan.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Shibata
authors.forename: Koichi
authors.initials: K
authors.lastname: Hiratsuka
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Hayakawa
authors.forename: Teruaki
authors.initials: T
authors.lastname: Shiroza
authors.forename: Hisashi
authors.initials: H
authors.lastname: Takiguchi
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Nagatsuka
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Abiko
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism* / Q000502:physiology; D001483:Base Sequence; D002352:Carrier Proteins / Q000378:metabolism* / Q000502:physiology; D000081062:Heme-Binding Proteins; D006420:Hemeproteins / Q000378:metabolism* / Q000502:physiology; D006427:Hemin / Q000378:metabolism*; D008969:Molecular Sequence Data; D016966:Porphyromonas gingivalis / Q000472:pathogenicity*; D011524:Protoporphyrins / Q000378:metabolism; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D000081062:Heme-Binding Proteins; D006420:Hemeproteins; D011524:Protoporphyrins; D037521:Virulence Factors; D006427:Hemin