Close correlation between restriction fragment length polymorphism of L-myc gene and susceptibility to gastric cancer.
OBJECTIVE
A common inherited RFLP of the L-myc proto-oncogene has been reported to correlate with cancer susceptibility. Our aim was to test the hypothesis that there was association between L-myc S allele in gastric cancer and predisposition to the disease.


METHODS
The distribution of L-myc polymorphism in 25 patients with gastric cancer was determined by polymerase chain reaction-based restriction fragment length polymorphism and compared with that of 83 healthy control subjects.


RESULTS
We found a significant difference, both in the distribution of the LL, LS and SS genotypes and in the allelic frequencies, between the control group and the patient group; that is, the frequencies of L-myc alleles were, L and S, 0.52 and 0.48, 0.64 and 0.36, respectively. This difference was primarily the result of a high frequency of the S allele among gastric cancer patients compared to controls. There was a significant difference in the distribution of both genotypes (P = 0.004) and allele frequencies (P = 0.005) between patients with gastric cancer and control groups.


CONCLUSIONS
Our results suggested that L-myc polymorphism may be significant in an individual's susceptibility to gastric cancer in Turkey and may be useful for identifying patients at high risk of developing gastric cancer.
Isbir
Turgay
T
Department of Molecular Medicine, Institute of Experimental Medical Research, University of Istanbul, DETAE 34390, P.O. Box 7 Capa, Istanbul, Turkey. tisbir@superonline.com.tr
Yaylim
Ilhan
I
Arikan
Soykan
S
Kaytan
Esra
E
Karşidağ
Tamer
T
Bayrak
Savaş
S
Camlica
Hakan
H
D000483:Alleles; D016022:Case-Control Studies; D004247:DNA / Q000097:blood / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005787:Gene Frequency; D016259:Genes, myc / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D000090063:Proto-Oncogene Mas; D013274:Stomach Neoplasms / Q000235:genetics*
D017931:DNA Primers; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D004247:DNA