title: GD3 expression in CHO-K1 cells increases growth rate, induces morphological changes, and affects cell-substrate interactions.
abstract: We have generated a panel of CHO-K1 cell clones with different glycolipid compositions by stable transfection of appropriate glycosyltransferases and studied the morphological and growth phenotype of a clone stably expressing Sial-T2. Compared with the GM3 expressing parental cells, Sial-T2 transfectants show low expression of GM3 and neo expression of GD3 and GT3. These cells show about 60% reduction of the mean cell area, and about 2-fold increase of the mean colony area and growth rate. Cells over expressing Sial-T2 showed a flattened appearance, and with time in culture they detached from the substrate leaving adhered material that was GD3 immunoreactive. No apoptotic or proteome differences could be detected in the Sial-T2 transfectants. Thus, increased expression of GD3 and GT3 influence parameters of growth and social behavior of CHO-K1 cells. However, the molecular and cellular basis underlying these influences requires further investigation.
authors.affiliation: CIQUIBIC (UNC-CONICET), Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Jose L
authors.initials: JL
authors.lastname: Daniotti
authors.forename: Adolfo R
authors.initials: AR
authors.lastname: Zurita
authors.forename: Vera M T
authors.initials: VM
authors.lastname: Trindade
authors.forename: Hugo J F
authors.initials: HJ
authors.lastname: Maccioni
mesh_terms: D000818:Animals; D016466:CHO Cells; D002455:Cell Division; D002999:Clone Cells; D006224:Cricetinae; D005732:Gangliosides / Q000378:metabolism*; D020543:Proteome
chemical_list: D005732:Gangliosides; D020543:Proteome; C026226:ganglioside, GD3