title: Use of real-time PCR to detect and quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" DNA.
abstract: A real-time PCR assay using Taqman probes was developed to detect and quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" in feline blood samples. The assay was rapid and sensitive and was successfully used to monitor the in vivo kinetics of cats experimentally infected with each species.
authors.affiliation: Department of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol, BS40 5DU, United Kingdom. s.tasker@bristol.ac.uk
authors.forename: Séverine
authors.initials: S
authors.lastname: Tasker
authors.forename: Chris R
authors.initials: CR
authors.lastname: Helps
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Day
authors.forename: Tim J
authors.initials: TJ
authors.lastname: Gruffydd-Jones
authors.forename: Dave A
authors.initials: DA
authors.lastname: Harbour
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000382:microbiology; D002415:Cats; D004269:DNA, Bacterial / Q000032:analysis; D004195:Disease Models, Animal; D007700:Kinetics; D009174:Mycoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000382:microbiology* / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial