Isolation and identification of two [(3)H]norharman- ([(3)H]beta-carboline)-binding proteins from rat liver.
Norharman (9H-pyrido-[3,4-b]indol) represents a member of the mammalian alkaloids with the group name beta-carbolines. In mammals, it exhibits psychotropic and co-mutagenic actions. Highly specific [(3)H]norharman binding sites have been detected in the liver of rats (B(max): 11 pmol mg(-1) protein; K(D): lower nanomolar range). Two [(3)H]norharman binding proteins with apparent molecular masses of 60 and 80 kDa (SDS-PAGE) were isolated from rat liver crude membrane fraction and identified as the enzyme carboxylesterase (EC 3.1.1.1; 60 kDa) and the stress protein glucose-regulated protein 78 (GRP78; 78 kDa). Possible functional consequences of the interaction of norharman with these two proteins are discussed.
Greube
Alexa
A
Free University of Berlin, Department of Clinical Neurobiology, Ulmenallee 30, D-14050, Berlin, Germany.
Rommelspacher
Hans
H
D000595:Amino Acid Sequence; D000818:Animals; D002243:Carbolines / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006247:Harmine / Q000031:analogs & derivatives* / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar; D017386:Sequence Homology, Amino Acid; D014316:Tritium
D002243:Carbolines; D011506:Proteins; D014316:Tritium; D006247:Harmine; C010262:norharman