Which sites react first? Functional site distribution and kinetics on solid supports investigated using confocal Raman and fluorescence microscopy.
Fluorescence microscopy is a powerful technique for analyzing beads with very low loadings of fluorophores; however, the method is flawed when looking at more highly loaded beads as a result of severe problems with absorption. To probe distributions at higher loading levels, Raman spectroscopy avoids many of these issues. These studies show that there is a uniform distribution of reactive sites throughout the beads but that the spatial distribution of reacted sites depends on the polymer type, with a fine balance between reaction and diffusion rate.
Kress
Jürgen
J
Department of Chemistry, University of Southampton, Southampton SO17 1BJ, United Kingdom.
Zanaletti
Riccardo
R
Rose
Abigail
A
Frey
Jeremy G
JG
Brocklesby
William S
WS
Ladlow
Mark
M
Bradley
Mark
M
D001665:Binding Sites; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000379:methods; D008863:Microspheres; D036103:Nanotechnology / Q000379:methods; D012117:Resins, Synthetic; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*
D012117:Resins, Synthetic