[Experimental study of the virus inhibitory effect of chuan-kang-ping granule].
OBJECTIVE
To explore the virus inhibitory effect of Chuan-Kang-Ping Granule.


METHODS
Using tissue culture technique, the maximal non-toxic concentration of the drugs in Hep-2 cell culture were measured and the virus inhibitory index of the drugs on the respiratory syncytial virus (RSV), the coxackie B3 virus (Cox B3) and the influenza A1 virus (A1) was calculated. In addition, the degrees of inhibition of the drugs on the cytopathic effect of viruses in cell culture was observed by changing sequence of adding the viruses and the drugs.


RESULTS
The maximal non-toxic concentration of Chuan-Kang-Ping Granule was 1:16. The virus inhibitory index on RSV, CoxB3, A1 was 8.8 and 2, respectively. The maximal non-toxic concentration of Shuang-Huang-Lian oral administration Liquid was 1:32. The virus inhibitory index on RSV, CoxB3, A1 was 8, 16 and 2, respectively. The degrees of cytopathic effect were different when changing sequence of adding the viruses and the drugs.


CONCLUSIONS
Chuan-Kang-Ping Granule inhibited the replication of RSV, CoxB3, A1 in cultured cells. The inhibitory effect of the drugs were the same or nearly the same with Shuang-Huang-Lian oral administration liquid. The mechanism of the inhibitory effects of the drugs on the viruses was probably the direct killing effect in vitro, or the drugs might inhibit some key link of the replication of the viruses in cultured cells.
Li
Z
Z
211th Hospital of PLA, Harbin 150080, China.
Dong
G
G
Li
Z
Z
D000998:Antiviral Agents / Q000494:pharmacology*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D029822:Enterovirus B, Human / Q000187:drug effects*; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects; D008113:Liver Neoplasms; D008826:Microbial Sensitivity Tests; D018113:Respiratory Syncytial Virus, Human / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D000998:Antiviral Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal