High-throughput purification of combinatorial arrays.
Performance and reproducibility of the Biotage Parallex, a high-throughput purification system, was evaluated using known standards. The results indicate that parallel purification is a robust technique for purifying large numbers of compounds. Results from one of the first libraries to be purified on the Biotage Parallex are presented and discussed. Since fractionation by UV can often result in a large number of fractions, threshold trigger versus yield and number of fractions was also investigated. This approach was used to purify an array of 4320 compounds, produced by an 11-step solid-phase synthesis in Irori MicroKans. Ninety-three percent of the compounds were successfully processed, with >90% having purity >95%.
Edwards
Christine
C
Biotage, Inc, A Dyax Corp Company, Charlottesville, Virginia 22902, USA. cedwards@biotage.com
Hunter
David J
DJ