[Impact on IL-2 expression of lymphocytes in donors after G-CSF administration and its clinical significance].
In this study, to investigate the effect on expression of IL-2 in lymphocytes from bone marrow and peripheral blood of normal donors after they were mobilized by G-CSF in allo-BMT, 7 normal donors bone marrow and peripheral blood were harvested before and after G-CSF administration. The separated lymphocytes were measured by FCM after they were stained intracellularly by anti-IL-2, and their expressions of IL-2 were compared. The degree of aGVHD in patients after bone marrow transplantation was evaluated clinically, and it was compared with the status of aGVHD of 15 patients whose donors didn't receive G-CSF administration in our department, and 2 groups of patients are comparable in age, types of diseases and status of donors. The results showed that the expression of IL-2 in lymphocytes in 7 G-CSF mobilized donors decreased significantly after G-CSF administration and more severe aGVHD than grade II didn't develop in these recipient patients, and comparing with 15 patients received the bone marrow from donors who didn't receive G-CSF, the incidence of aGVHD decreased. It is suggested that the expression of IL-2 in lymphocytes was influenced by donors' G-CSF administration, and it is likely that thereby reduces the incidence of aGVHD in patients after BMT.
Jia
Ye-Hui
YH
Xijing Hospital, The Fourth Military University, Xi'an 710032, China.
Ji
Shu-Quan
SQ
Lai
Chun-Ning
CN
Chen
Hui-Ren
HR
Yu
Ming
M
Li
Yan
Y
Shen
Ben-Fen
BF
D000328:Adult; D001782:Blood Donors; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D005260:Female; D005434:Flow Cytometry; D006086:Graft vs Host Disease / Q000209:etiology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male
D007376:Interleukin-2; D016179:Granulocyte Colony-Stimulating Factor