Global loss of Na,K-ATPase and its nitric oxide-mediated regulation in a transgenic mouse model of amyotrophic lateral sclerosis.
Na,K-ATPase plays a critical role in energy metabolism and ion fluxes. Its loss was investigated in the G93A mouse model of amyotrophic lateral sclerosis (ALS) in which the mutation of Cu/Zn superoxide dismutase (SOD1) is thought to lead to aberrant oxidative damage. Observed losses in spinal cord Na,K-ATPase activity exceeded all expectations. All three catalytic subunit isoforms (alpha1, alpha2, alpha3) were reduced, and the global alpha subunit loss affected not just neurons, glia, and myelinated axon tracts but even ependymal and pial membranes. Decreases in Na,K-ATPase activity were greater than losses of protein, and there were losses of Na,K-ATPase alpha, but not beta, subunits. Together, these observations are consistent with selective degradation of the alpha subunit after damage. Overexpression of normal SOD1 does not cause ALS-like symptoms, but it has other known pathological effects. In transgenic mice overexpressed normal human SOD1 had a smaller but still considerable effect on Na,K-ATPase. Furthermore, the nitric oxide-mediated regulatory pathway for Na,K-ATPase inhibition was undetectable in spinal cord tissue slices from mice overexpressing either mutant or normal human SOD1. Na,K-ATPase activity did not respond to nitric oxide donors, and the free radical-dependent step of the pathway could not be bypassed by the addition of the downstream protein kinase G activator, 8-Br-cGMP. The data demonstrate that Na,K-ATPase is vulnerable to aberrant SOD1 activity, making it a potential contributing factor in disease pathology. Moreover, the global cellular distribution of Na,K-ATPase loss indicates that SOD1 overexpression is far-reaching in its pathological effects.
Ellis
Dorette Z
DZ
The Neuroscience Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA.
Rabe
Jason
J
Sweadner
Kathleen J
KJ
D000690:Amyotrophic Lateral Sclerosis / Q000201:enzymology*; D000818:Animals; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D020125:Mutation, Missense; D009569:Nitric Oxide / Q000502:physiology*; D010042:Ouabain / Q000494:pharmacology; D020033:Protein Isoforms / Q000032:analysis; D021122:Protein Subunits; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis* / Q000276:immunology / Q000378:metabolism*; D013116:Spinal Cord / Q000187:drug effects / Q000201:enzymology; D013482:Superoxide Dismutase / Q000235:genetics*; D000072105:Superoxide Dismutase-1
D005609:Free Radicals; D020033:Protein Isoforms; D021122:Protein Subunits; C000606290:SOD1 protein, human; D009569:Nitric Oxide; D010042:Ouabain; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D000254:Sodium-Potassium-Exchanging ATPase