title: Region specificity of chromosome III on gene expression in the yeast Saccharomyces cerevisiae.
abstract: A single copy of a reporter gene cassette, such as PGKP-lacZ-LEU2 (promoter-reporter-marker gene) cassette, was inserted into one of 32 positions along chromosome III in Saccharomyces cerevisiae with an interval of approximately 10 kb. The amounts of translational gene product (beta-galactosidase) synthesized by the cassette-transformed cells were then determined. The region specificity in chromosome III could be demonstrated in gene expression: two higher-expressed regions (hot regions), 133 and 199 (MAT) regions, and seven lower-expressed regions (cold regions). For the steady and high production of polypeptide, foreign gene products, by yeast, we would like to state that we hope for an insertion of the artificially prepared gene cassette [(promoter)-(foreign gene)-(marker gene) ] into a hot region, such as 199 (MAT) region of chromosome III.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Engineering, Fukuyama University, Fukuyama 729-0292, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Yamane
authors.forename: Masaki
authors.initials: M
authors.lastname: Yamaoka
authors.forename: Mami
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Maruki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Matsuzaki
authors.forename: Takushi
authors.initials: T
authors.lastname: Hatano
authors.forename: Sakuzo
authors.initials: S
authors.lastname: Fukui