title: Factor V Leiden and factor II G20210A in preeclampsia and HELLP syndrome.
abstract: BACKGROUND
  The association of factor V and factor II mutations with preeclampsia and hemolysis, elevated liver enzymes, and low platelets (HELLP) syndrome, and a possible role of the two thrombophilic mutations in the pathogenesis of the diseases have been previously investigated. The results, however, are still inconclusive and contradictory.
  
  
  METHODS
  A case-control study was performed over an interval of 24 months, on 111 subjects with preeclampsia and 111 normal pregnant women matched for age and parity, without previous thromboembolic disorders. The subjects were tested for the mutation A1691G in the factor V gene (R506Q or Leiden mutation) and the mutation A20210G in the factor II (prothrombin) gene. The Student's t-test and the chi2-test were applied when appropriate, and odds ratios and 95% confidence intervals were calculated.
  
  
  RESULTS
  Fourteen patients with preeclampsia (12.6%) had at least one of the two mutations, as compared with six controls (5.4%). Factor V Leiden was found in eight patients with preeclampsia (7.2%) and in five controls (4.5%). Factor II G20210A was detected in eight preeclamptic women (7.2%) and in one normal pregnant woman (0.9%)(p = 0.041). In the subgroup of 32 preeclamptic subjects with HELLP syndrome, factor V Leiden was found in three patients (9.3%) and factor II G20210A in two (6.2%).
  
  
  CONCLUSIONS
  The prevalence of factor V and factor II mutations is increased in patients with preeclampsia; the thrombophilic mutations may interact with other pathogenic factors to determine the clinical features of the disease and of its complications.
authors.affiliation: Department of Gynecology and Obstetrics, University of Turin, Via Baiardi 43, 10126 Turin, Italy. chbened@tin.it
authors.forename: Chiara
authors.initials: C
authors.lastname: Benedetto
authors.forename: Luca
authors.initials: L
authors.lastname: Marozio
authors.forename: Loredana
authors.initials: L
authors.lastname: Salton
authors.forename: Vincenza
authors.initials: V
authors.lastname: Maulà
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Chieppa
authors.forename: Marco
authors.initials: M
authors.lastname: Massobrio
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004252:DNA Mutational Analysis; D005165:Factor V / Q000235:genetics*; D005260:Female; D017359:HELLP Syndrome / Q000235:genetics* / Q000503:physiopathology*; D006801:Humans; D011247:Pregnancy; D015995:Prevalence; D011516:Prothrombin / Q000235:genetics*; D012307:Risk Factors; D019851:Thrombophilia / Q000235:genetics
chemical_list: C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin