Characterization of proteins from boar prostate.
OBJECTIVE
Most components of seminal plasma are secreted by accessory sexual glands: seminal vesicle, prostate gland and bulbourethral gland. The portion of proteins secreted by prostate gland differs in various species. Characterization of boar prostate proteins is the subject of this communication.


METHODS
Proteins of boar prostate gland were separated by affinity chromatography on heparin-polyacrylamide to non-heparin-binding (H-) and heparin-binding (H+) fractions. The H- and H+ fractions were subjected to reverse phase high performance liquid chromatography (RP HPLC) and their elution profiles were compared with those of the H- and H+ fractions of boar seminal plasma. The isolated proteins were characterized by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), immunodetection, N-terminal amino acid sequencing and mass spectrometry (MALDI).


RESULTS
The following proteins of boar prostate secretion were identified: beta-microseminoprotein, serotransferrin, serum albumin, myoglobin and PSP I and PSP II spermadhesins.


CONCLUSIONS
Presented results demonstrate composition of the main proteins of boar prostate secretion. Beta-Microseminoprotein was found to be a major protein of prostate secretion. PSP I and PSP II, major proteins of the H- fraction of boar seminal plasma, were found in boar prostate secretion in lower amounts. The major proteins of the H+ fraction of boar seminal plasma (AQN, AWN) were not detected in prostate secretion.
Manásková
Pavla
P
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic. pavlam@img.cas.cz
Rylava
Helena
H
Tichá
Marie
M
Jonáková
Vera
V
D000818:Animals; D006493:Heparin / Q000378:metabolism; D008297:Male; D009211:Myoglobin / Q000302:isolation & purification / Q000378:metabolism; D011467:Prostate / Q000378:metabolism*; D029584:Prostatic Secretory Proteins / Q000302:isolation & purification / Q000378:metabolism; D029589:Seminal Plasma Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012709:Serum Albumin / Q000302:isolation & purification / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine / Q000378:metabolism*; D014168:Transferrin / Q000302:isolation & purification / Q000378:metabolism
D009211:Myoglobin; D029584:Prostatic Secretory Proteins; D029589:Seminal Plasma Proteins; D012709:Serum Albumin; D014168:Transferrin; C043076:beta-microseminoprotein; C425042:spermadhesin; D006493:Heparin