Nuclear factor-kappaB inhibitors abolish hypoxic vasoconstriction in sheep-isolated pulmonary arteries.
The aim of this study was to determine the role of nuclear factor-kappaB (NF-kappaB) in hypoxic constriction of isolated pulmonary arteries. Rings were suspended in an organ bath filled with Krebs-Henseleit solution and isometric contractions were recorded continuously. Hypoxia (%95 N(2)-%5 CO(2)) had no marked effect on resting force in artery rings. However, hypoxia caused further contractions in serotonin-precontracted arteries. Hypoxia-induced vasoconstrictions were abolished by preincubation with NF-kappaB inhibitors, pyrrolidine dithiocarbamate (100 microM) or pyrithione (10 microM). These results suggest that reactive oxygen species and/or NF-kappaB activation may be involved in the hypoxia-induced vasoconstriction in sheep-isolated pulmonary arteries.
Uzun
Ozge
O
Department of Pharmacology, Faculty of Medicine, Abant izzet Baysal University, 14450 Konuralp, Düzce, Turkey.
Demiryürek
A Tuncay
AT
D000818:Animals; D000860:Hypoxia / Q000503:physiopathology*; D066298:In Vitro Techniques; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011651:Pulmonary Artery / Q000187:drug effects* / Q000502:physiology; D011725:Pyridines / Q000494:pharmacology; D011759:Pyrrolidines / Q000494:pharmacology*; D012701:Serotonin / Q000494:pharmacology; D012756:Sheep; D013859:Thiocarbamates / Q000494:pharmacology*; D013871:Thiones; D014661:Vasoconstriction / Q000187:drug effects*
D016328:NF-kappa B; D011725:Pyridines; D011759:Pyrrolidines; D013859:Thiocarbamates; D013871:Thiones; C020972:pyrrolidine dithiocarbamic acid; D012701:Serotonin; C008704:pyrithione