title: Cytokinin affinity purification and identification of a tobacco BY-2 adenosine kinase.
abstract: Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be disrupted by several other adenine- but not guanine-based purine derivatives. The observed interaction with cytokinins is discussed in view of a putative role for adenosine kinase in TBY-2 cytokinin metabolism. The presented results show for the first time a plant adenosine kinase affinity-purified to homogeneity that was identified by primary structure analysis.
authors.affiliation: Laboratorium voor Plantenbiochemie en -fysiologie, Department of Biology, University of Antwerp (UIA), Universiteitsplein 1, B-2610, Antwerp, Belgium. kris.laukens@ua.ac.be
authors.forename: Kris
authors.initials: K
authors.lastname: Laukens
authors.forename: René
authors.initials: R
authors.lastname: Lenobel
authors.forename: Miroslav
authors.initials: M
authors.lastname: Strnad
authors.forename: Harry
authors.initials: H
authors.lastname: Van Onckelen
authors.forename: Erwin
authors.initials: E
authors.lastname: Witters
mesh_terms: D000248:Adenosine Kinase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D003583:Cytokinins / Q000378:metabolism; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012995:Solubility; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics; D015026:Zeatin / Q000378:metabolism
chemical_list: D003583:Cytokinins; D015026:Zeatin; D000248:Adenosine Kinase