Transcription initiation activity of adenovirus left-end sequence in adenovirus vectors with e1 deleted.
We analyzed the transcription initiation activity of the left-end sequence (first 342 bp) of the adenovirus genome in the context of an adenovirus vector with E1 deleted in in vitro and in vivo gene transfer models. While nucleotide sequences 1 to 190 and 1 to 342 showed strong activity in three out of three lung cancer cell lines, nucleotide sequence 1 to 103 showed limited activity in H358, cells which show characteristics of type 2 alveolar cells. In vivo, the transcription initiation activities of nucleotide sequence 1 to 103 in the liver and the lung were minimal, while nucleotide sequences 1 to 190 and 1 to 342 showed strong activity comparable to that of the cytomegalovirus promoter. Further understanding of the transcription initiation activity of the left-end sequence of the adenovirus genome should lead to optimization of adenovirus vectors.
Yamamoto
Masato
M
Division of Human Gene Therapy, Department of Medicine, and the Gene Therapy Center, University of Alabama at Birmingham, 35294-2172, USA. Masato.Yamamoto@ccc.uab.edu
Davydova
Julia
J
Takayama
Koichi
K
Alemany
Ramon
R
Curiel
David T
DT
D000256:Adenoviridae / Q000235:genetics*; D017498:Adenovirus E1 Proteins / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004261:DNA Replication; D005260:Female; D005822:Genetic Vectors; D051379:Mice; D008807:Mice, Inbred BALB C; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D017498:Adenovirus E1 Proteins; D017931:DNA Primers