Simultaneous detection of ACP1 and GC genotypes using PCR/SSCP.
The classical enzyme and protein markers ACP1 and GC have gained new importance because of the biological functions of their gene products. ACP1 encodes a low molecular weight enzyme which is now recognized as a phosphotyrosine phosphatase with a role in the regulation of signal transduction pathways, and GC-globulin acts both as a transporter of vitamin D and as a plasma actin scavenger and plays a role in macrophage activation. These two polymorphisms were phenotyped for decades on the basis of electrophoretic isozyme or protein patterns; the gene structures are now known. Nucleotide substitutions determining the common alleles are close enough at each locus to be contained in one short PCR product. We have developed a simple, rapid and reliable multiplex method based on PCR and SSCP which allows the simultaneous determination of the common ACP1 and GC genotypes.
Dissing
J
J
Institute of Forensic Medicine, University of Copenhagen, Denmark. joergen.dissing@forensic.ku.dk
Thymann
M
M
Hopkinson
D
D
D000483:Alleles; D017931:DNA Primers; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D007527:Isoenzymes; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D011518:Proto-Oncogene Proteins; D014809:Vitamin D-Binding Protein / Q000235:genetics* / Q000502:physiology
D017931:DNA Primers; D005819:Genetic Markers; D007527:Isoenzymes; D011518:Proto-Oncogene Proteins; D014809:Vitamin D-Binding Protein; C490429:ACP1 protein, human; D017027:Protein Tyrosine Phosphatases