[Expression and characterization of Kringle 1-4.5 domains of human plasminogen].
The cDNA encoding Kringle 1-4 and part of Kringle 5 domains of human plasminogen (K1-4.5), obtained from HepG2 by RT-PCR, was cloned into expression vector pHIL-S1. The recombinant plasmid pHIL-K1-4.5 was transformed into Pichi pastoris GS115 and the recombinant yeast was induced to express the recombinant proteins by methanol. The expressed proteins were purified by lysine affinity chromatography to a purity of 95%. The recombinant K1-4.5 inhibited the growth of bovine capillary endothelial cells (BAEC) stimulated by the basic fibroblast growth factor (bFGF), in a dosage-dependent manner with a half maximal concentration of 2 mg/L. rhK1-4.5 also inhibited 40% of the BAEC migration stimulated by bFGF in the concentration of 1 mg/L.
Zhou
Qing-Wei
QW
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China.
Xie
Jing-Li
JL
Xin
Li
L
Xu
Ren
R
Ye
Qing
Q
Li
Zai-Ping
ZP
Gan
Ren-Bao
RB
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D018082:Kringles / Q000235:genetics*; D010843:Pichia / Q000235:genetics; D010958:Plasminogen / Q000737:chemistry / Q000235:genetics*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2; D010958:Plasminogen