Expression and role of thrombospondin-1 in colorectal cancer.
Thrombospondin-1 (TSP1) inhibits angiogenesis and activates transforming growth factor beta-1 (TGF beta-1). The expression and role of TSP1 remain controversial. On 132 colorectal cancer specimens, we performed immunohistochemical staining of TSP1, TGF beta-1, latency-associated peptide (LAP) and CD34, besides performing in situ hybridization (ISH) of TSP1. TSP1 was mainly localised in fibroblasts of the tumor stroma on ISH. The result revealed that 73 cases (55.3%) were evaluated as high-TSP1 while 84 cases (63.6%) were evaluated as high-TGF beta-1. The expression of TSP1 correlated significantly with vessel counts (p = 0.016) and TGF beta-1 expression (p = 0.043). Cox proportional hazard analysis showed that TSP1 expression was significantly correlated with independent prognostic factors. The present study furnishes evidence indicating that TSP1 is expressed in tumor stroma, inhibits tumor angiogenesis and suppresses tumor growth by activating TGF beta-1.
Miyanaga
Katsuya
K
Second Department of Surgery, Fukui Medical University, Matsuoka-Cho, Yoshida-Gun, Fukui 910-1104, Japan. legend@fmsrsa.fukuimed.ac.jp
Kato
Yasushi
Y
Nakamura
Tomoaki
T
Matsumura
Mitsuyosi
M
Amaya
Hirokazu
H
Horiuchi
Tetsuya
T
Chiba
Yukio
Y
Tanaka
Kuniyoshi
K
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000378:metabolism; D010446:Peptide Fragments / Q000096:biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis; D019700:Thrombospondin 1 / Q000096:biosynthesis / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis; D053773:Transforming Growth Factor beta1
D010446:Peptide Fragments; D011498:Protein Precursors; C508960:TGFB1 protein, human; D019700:Thrombospondin 1; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1