Self-assembled DNA-conjugated polymer for novel DNA chip.
We developed DNA-conjugated polymer for DNA chip fabrication. A 30 mer probe DNA and disulfide bridges were covalently attached to the polymer side chain. The DNA-conjugated polymer can be specifically adsorbed on a gold substrate surface by a self-assembly technique. The interaction between fully matched DNA and DNA-conjugated polymer was investigated by surface plasmon resonance (SPR) technique. The DNA-conjugated polymer-modified gold surface highly recognized fully matched DNA, rather than unmatched DNA. Therefore, DNA-conjugated polymer can be used for novel DNA chip fabrication.
Taira
Shu
S
Laboratory of Advanced Bioelectronics, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 4, 1-1-1 Higashi, Tsukuba 305-8562, Japan.
Morita
Yasutaka
Y
Tamiya
Eiichi
E
Yokoyama
Kenji
K
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D006863:Hydrogen-Ion Concentration; D008904:Miniaturization; D009693:Nucleic Acid Hybridization; D011108:Polymers / Q000737:chemistry*; D020349:Surface Plasmon Resonance
D015342:DNA Probes; D011108:Polymers; D004247:DNA