title: In vitro parasiticidal effect of Nitazoxanide against Echinococcus multilocularis metacestodes.
abstract: When humans serve as inadvertent intermediate hosts for Echinococcus multilocularis, disease (alveolar echinococcosis [AE]) may result from the expanding parasite metacestode in visceral organs, mostly in the liver. Benzimidazole carbamate derivatives such as mebendazole and albendazole are used for chemotherapeutic treatment of AE. However, these treatments are, in most cases, parasitistatic rather than parasiticidal. As treatment is discontinued, a recurrence of parasite growth has been observed in many AE patients with nonradical resections. The only curative treatment for AE is radical surgical resection of the parasite tissue and support by chemotherapy. As there is a need for new treatment options for AE, the in vitro efficacy of nitazoxanide (NTZ), a broad-spectrum drug used against intestinal parasites and bacteria, was investigated. We showed that in vitro treatment of E. multilocularis metacestodes with NTZ induced high levels of alkaline phosphatase activity in the medium. Concurrently, distinct morphological and ultrastructural alterations were detected. Most significantly, two distinct types of alterations were observed as soon as after 3 h of NTZ treatment. At first, the drug induced a peripheral output of membranous vesicles from the tegumental membrane into the laminated layer. Simultaneously, germinal layer-associated undifferentiated cells produced large vacuoles filled with lipid-like and often electron-dense membranous segments. Other alterations were observed at later time points, including vacuolization of the germinal layer, accumulation of lipid droplets, and lastly, loss of microtriches and separation of the laminated and germinal layers. The pattern of damage induced by NTZ was different from the alterations earlier observed in albendazole sulfoxide-treated vesicles. The nonviability of NTZ-treated metacestodes was confirmed through bioassay, i.e., inoculation of treated and untreated parasites into mice. These experiments demonstrate the in vitro parasiticidal effect of NTZ on E. multilocularis metacestodes.
authors.affiliation: Institute of Parasitology, Faculties of Veterinary Medicine and Medicine, University of Berne, CH-3012 Berne, Switzerland.
authors.forename: Marianne
authors.initials: M
authors.lastname: Stettler
authors.forename: Renate
authors.initials: R
authors.lastname: Fink
authors.forename: Mirjam
authors.initials: M
authors.lastname: Walker
authors.forename: Bruno
authors.initials: B
authors.lastname: Gottstein
authors.forename: Timothy G
authors.initials: TG
authors.lastname: Geary
authors.forename: Jean François
authors.initials: JF
authors.lastname: Rossignol
authors.forename: Andrew
authors.initials: A
authors.lastname: Hemphill
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000923:Anticestodal Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002478:Cells, Cultured; D004443:Echinococcosis / Q000188:drug therapy / Q000473:pathology; D004446:Echinococcus / Q000187:drug effects* / Q000201:enzymology; D005260:Female; D005849:Gerbillinae; D051379:Mice; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D017348:Microscopy, Electron, Scanning Transmission; D009574:Nitro Compounds; D013844:Thiazoles / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000923:Anticestodal Agents; D009574:Nitro Compounds; D013844:Thiazoles; D000469:Alkaline Phosphatase; C041747:nitazoxanide