Neural differentiation of mouse embryonic stem cells.
Pluripotent embryonic stem cells can give rise to neuroectodermal derivatives in culture. This potential could be harnessed to generate neurons and glia for cell-replacement therapies in the central nervous system and for use in drug discovery. However, current methods of neural differentiation are empirical and relatively inefficient. Here, we review these methodologies and present new tools for quantification, analysis and manipulation of embryonic stem cell neural determination.
Stavridis
M P
MP
Institute for Stem Cell Research, University of Edinburgh, The King's Buildings, West Mains Road, Edinburgh EH9 3JQ, UK.
Smith
A G
AG
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D019070:Cell Lineage; D003470:Culture Media; D004622:Embryo, Mammalian / Q000166:cytology*; D051379:Mice; D008954:Models, Biological; D009474:Neurons / Q000473:pathology*; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors
D003470:Culture Media