title: Evaluation of lymphocyte populations and subpopulations extracted from inflamed periodontal tissues.
abstract: The aim of the present study was evaluation the distribution of B- and T-cells and T-cell subsets in periodontal tissues from patients with different periodontitis forms. Periodontal tissue samples were collected from group P patients during routine surgical procedures, while from group C during tooth extraction for orthodontic or prosthetic purposes. Directly after collection, tissue samples were placed in a criostat or fixed in 10% buffered formalin for 24 h at room temperature. Following fixation the material was embedded in paraffin and subjected to routine histological techniques. Examinations of B- and T-lymphocytes populations and T-lymphocytes subsets were made with the use of immunohistochemical method. In C group single T and B lymphocytes were found in histological examination in pocket epithelium zones. In early onset periodontitis (EOP) patients in inflammatory infiltration lymphocytes T were dominating while in adult periodontitis (AP) patients dominating were B lymphocytes. Mean CD4/CD8 ratio in control group was 1.7 and in EOP and AP patients 1.1 and 2.6 respectively.
authors.affiliation: Department of Conservative Dentistry, Medical Academy of Białystok, Białystok, Poland.
authors.forename: Małgorzata D
authors.initials: MD
authors.lastname: Pietruska
authors.forename: Lech
authors.initials: L
authors.lastname: Zimnoch
authors.forename: Danuta
authors.initials: D
authors.lastname: Waszkiel
authors.forename: Wanda
authors.initials: W
authors.lastname: Stokowska
authors.forename: Ewa
authors.initials: E
authors.lastname: Duraj
mesh_terms: D000328:Adult; D016175:B-Lymphocyte Subsets / Q000502:physiology*; D016516:CD4-CD8 Ratio; D016022:Case-Control Studies; D046508:Culture Techniques; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000175:diagnosis* / Q000473:pathology; D011336:Probability; D012016:Reference Values; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D016176:T-Lymphocyte Subsets / Q000502:physiology*
chemical_list: D018836:Inflammation Mediators