[Ligands with specific affinity to a specific sequence of DNA base pairs. XI. The synthesis and binding to DNA of bis-netropsins with the C-ends of their netropsin fragments tethered by tetra- or pentamethylene linkers].
Bis-Netropsins with the C-ends of their netropsin fragments tethered via tetra- or pentamethylene linkers and with Gly or L-Lys-Gly residues on their N-ends were synthesized. The footprinting technique was used to study the specificity of bis-netropsin binding to the specially constructed DNA fragments containing various clusters of A.T pairs. It was found that the linker length affects the binding of bis-netropsins, with the tetramethylene linker providing better protection than the pentamethylene linker. It was shown that the newly synthesized bis-netropsins bind tighter to the 5'-A4T(4)-3' sequence, whereas the bis-netropsin with a linker between the netropsin N-ends binds better to 5'-T4A(4)-3' sequences. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2002, vol. 28, no. 6; see also http://www.maik.ru.
Grokhovskiĭ
S L
SL
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow Center of Medical Research of Oslo University, Moscow, Russia. grokhovs@imb.ac.ru
Nikolaev
V A
VA
Gottikh
B P
BP
Zhuze
A L
AL
D020029:Base Pairing; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D018983:DNA Footprinting; D008024:Ligands; D008969:Molecular Sequence Data; D009429:Netropsin / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry*; D017422:Sequence Analysis, DNA
D008024:Ligands; D009429:Netropsin; C032161:bis-netropsin; D004247:DNA