title: Crystallization and preliminary X-ray diffraction analysis of the di-haem cytochrome c peroxidase from Pseudomonas stutzeri.
abstract: Crystals of cytochrome c peroxidase from Pseudomonas stutzeri were obtained using sodium citrate and PEG 8000 as precipitants. A complete data set was collected to a resolution of 1.6 A under cryogenic conditions using synchrotron radiation at the ESRF. The crystals belong to space group P2(1), with unit-cell parameters a = 69.29, b = 143.31, c = 76.83 A, beta = 100.78 degrees. Four CCP molecules were found in the asymmetric unit, corresponding to a pair of dimers related by local dyads. The crystal packing in the structure shows that the functional dimers can dimerize, as suggested by previous biochemical studies.
authors.affiliation: REQUIMTE, Centro de Química Fina e Biotecnologia, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Monte de Caparica, Portugal.
authors.forename: Cecília
authors.initials: C
authors.lastname: Bonifácio
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Cunha
authors.forename: Axel
authors.initials: A
authors.lastname: Müller
authors.forename: Cristina G
authors.initials: CG
authors.lastname: Timóteo
authors.forename: João M
authors.initials: JM
authors.lastname: Dias
authors.forename: Isabel
authors.initials: I
authors.lastname: Moura
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Romão
mesh_terms: D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D003578:Cytochrome-c Peroxidase / Q000737:chemistry*; D019281:Dimerization; D006418:Heme / Q000737:chemistry*; D008958:Models, Molecular; D011549:Pseudomonas / Q000201:enzymology*; D017356:Synchrotrons
chemical_list: D006418:Heme; D003578:Cytochrome-c Peroxidase