[Analysis on integration sites of transposon-insertion mutant by transposon rescue].
In order to define the chromosomal locus of the transposon integration in a mutant of Corynebacterium glutamicum, transposon rescue experiments were performed. By isolating parts of the transposon together with adjacent parts of the bacterial chromosome and subsequent sequencing of the adjacent parts, the mutant was found to have three transposon integrations, one just in front of the citrate synthase gene gltA, the other two in hitherto unknown open reading frames designated orfA and orfB. The transposon rescue experiments are proven to be a easy, practical and ideal way in analyzing the transposon integration sites.
Ruan
Hong
H
College of Life Science, Zhejiang University, Hangzhou 310027, China.
Eikmanns
Bernhard
B
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002950:Citrate (si)-Synthase / Q000235:genetics; D003352:Corynebacterium / Q000201:enzymology / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C104631:ORFA protein, Corynebacterium; D002950:Citrate (si)-Synthase