Bone regeneration following injection of mesenchymal stem cells and fibrin glue with a biodegradable scaffold.
OBJECTIVE
The purpose of this study was to determine whether a combination of fibrin glue, beta-tricalcium phosphate as a biodegradable (beta-TCP) and mesenchymal stem cells would provide three-dimensional templates for bone growth resulting in new bone formation at heterotopic sites in the rat with plasticity.


METHODS
Growing stem cells and developing matrices, explanted from the rat femur, were fragmented and mixed with fibrin glue in a syringe. The cells/beta-TCP fibrin glue admixtures were injected into the subcutaneous space on the dorsum of the rat.


RESULTS
Eight weeks after implantation, gross morphology revealed a pearly opalescence and firm consistency. Histological inspections showed newly formed bone structures in all admixtures, but none in the control groups when only fibrin glue and beta-TCP were injected. Osteopontin, a protein important in bone development, was identified by using antibodies in all cells/beta-TCP fibrin glue admixtures.


CONCLUSIONS
Mesenchymal stem cells/beta-TCP fibrin glue admixtures can result in successful bone formation. This technique holds the promise of a minimally invasive means of generating autogenous bone to correct or reconstruct bony defects.
Yamada
Yoichi
Y
Department of Oral and Maxillofacial Surgery, Nagoya University, Graduate School of Medicine, Japan
Boo
Jae Seong
JS
Ozawa
Ryotaro
R
Nagasaka
Tetsuro
T
Okazaki
Yasuhiro
Y
Hata
Ken-ichiro
K
Ueda
Minoru
M
D000818:Animals; D000906:Antibodies; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000627:therapeutic use*; D002130:Calcium Phosphates / Q000627:therapeutic use*; D002516:Ceramics / Q000627:therapeutic use*; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D008297:Male; D016555:Matched-Pair Analysis; D008648:Mesoderm / Q000166:cytology / Q000502:physiology*; D009999:Ossification, Heterotopic / Q000378:metabolism / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D053495:Osteopontin; D010750:Phosphoproteins / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D012795:Sialoglycoproteins / Q000032:analysis; D013234:Stem Cells / Q000502:physiology*; D014014:Tissue Adhesives / Q000627:therapeutic use*
D000906:Antibodies; D018786:Bone Substitutes; D002130:Calcium Phosphates; D015718:Fibrin Tissue Adhesive; D010750:Phosphoproteins; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D014014:Tissue Adhesives; C485817:beta-tricalcium phosphate; D053495:Osteopontin; C018392:tricalcium phosphate