Immunohistochemical localization of the sigma1-receptor in oligodendrocytes in the rat central nervous system.
By using a new polyclonal antibody raised against a 21-amino acid peptide sequence corresponding to the fragment 138-157 of the cloned rat sigma(1)-receptor, we demonstrated by immunoperoxidase and double immunofluorescence techniques, that rat oligodendrocytes express the sigma(1)-receptor. Experiments in vivo and in vitro showed that sigma(1)-receptor colocalized with specific markers of progenitor (A2B5) and mature oligodendrocytes (GalC, RIP). These results suggest that sigma(1)-receptor in oligodendrocytes might be involved in myelination by direct implication in cholesterol biosynthesis or by interaction with endogenous ligands such as neurosteroids.
Palacios
Gabriel
G
Histopathology Unit, Research Center, Laboratorios del Dr. Esteve, S.A., Av. Mare de Déu de Montserrat 221, 08041 Barcelona, Spain. gpalacios@esteve.es
Muro
Asunción
A
Vela
José Miguel
JM
Molina-Holgado
Eduardo
E
Guitart
Xavier
X
Ovalle
Sergio
S
Zamanillo
Daniel
D
D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D005699:Galactosylceramides / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009836:Oligodendroglia / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017480:Receptors, sigma / Q000378:metabolism*; D014018:Tissue Distribution; D000097605:Sigma-1 Receptor
D000911:Antibodies, Monoclonal; D005699:Galactosylceramides; D011506:Proteins; D017480:Receptors, sigma; D000097605:Sigma-1 Receptor; C002454:galactocerebroside