title: pO(2) measurements by phosphorescence quenching: characteristics and applications of an automated system.
abstract: An automated system for pO(2) analysis based upon phosphorescence quenching was tested. The system was calibrated in vitro with capillary samples of saline and blood. Results were compared to a conventional measuring procedure wherein pO(2) was calculated off-line by computer fitting of phosphorescence decay signals. PO(2) measurements obtained by the automated system were correlated (r(2) = 0.98) with readings simultaneously generated by a blood gas analyzer, accuracy being highest in the low (0-20 mm Hg) and medium pO(2) ranges (21-70 mm Hg). Measurements in in vivo studies in the hamster skin-fold preparation were similar to previously reported results. The automated system fits the phosphorescence decay data to a single exponential and allows repeated pO(2) measurements in rapid sequence.
authors.affiliation: Department of Anesthesiology, University Hospital of Mannheim, D-68135 Mannheim, Germany.
authors.forename: Heinz
authors.initials: H
authors.lastname: Kerger
authors.forename: Gesine
authors.initials: G
authors.lastname: Groth
authors.forename: Armin
authors.initials: A
authors.lastname: Kalenka
authors.forename: Peter
authors.initials: P
authors.lastname: Vajkoczy
authors.forename: Amy G
authors.initials: AG
authors.lastname: Tsai
authors.forename: Marcos
authors.initials: M
authors.lastname: Intaglietta
mesh_terms: D000818:Animals; D001331:Automation; D001784:Blood Gas Analysis / Q000379:methods / Q000706:statistics & numerical data; D006224:Cricetinae; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008647:Mesocricetus; D008833:Microcirculation / Q000737:chemistry*; D010100:Oxygen / Q000032:analysis* / Q000097:blood
chemical_list: D010100:Oxygen