Synthesis of oligonucleotides with a 2'-cap at the 3'-terminus via reversed phosphoramidites.
[reaction: see text] A method is presented for the synthesis of single compounds or small combinatorial libraries of oligonucleotides with 2'-acylamido-2'-deoxyuridine residues at the 3'-terminus. Selection experiments identified the residue of anthraquinone-2-carboxylic acid as a "molecular cap" that increases the UV melting point of the duplex (5'-ACGCGU-3')(2) by up to 28 degrees C compared to the unmodified control duplex.
Connors
William H
WH
Department of Chemistry, University of Constance, 78457 Konstanz, Germany.
Narayanan
Sukunath
S
Kryatova
Olga P
OP
Richert
Clemens
C
D001483:Base Sequence; D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000235:genetics; D009943:Organophosphorus Compounds / Q000737:chemistry*
D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D004247:DNA