Determination of eight selected periodontal pathogens in the subgingival plaque of maxillary first molars in Japanese school children aged 8-11 years.
To prevent the onset or progression of periodontitis, we must understand when periodontal pathogens are first harbored and how they develop the biofilm that causes periodontal disease. The purpose of this study was to determine the relationship between clinical status and selected periodontal pathogens in subgingival plaque in school children. This study was conducted with 95 school children, 8-11 years old. The presence and distribution of eight selected periodontal pathogens sampled from the maxillary right first molar were determined by an indirect immunofluorescent technique and compared with clinical parameters. Of the 95 sites sampled, only one site had all eight pathogens and five sites did not have any of the eight pathogens. The mean number of positive pathogens per site was 3.5 +/- 1.8 and mean percentage of positive pathogens was 3.82 +/- 4.22%. The number and total percentage of positive pathogens were strongly correlated with the Plaque Index (PI). In addition, the number of positive pathogens was correlated to the presence of subgingival calculus. The most frequently found pathogens were Campylobacter rectus (84.2%) and Eikenella corrodens (83.2%), and the least, Actinobacillus actinomycetemcomitans serotype c (7.4%). Of the eight pathogens, the frequency and distribution of Porphyromonas gingivalis were significantly correlated with PI and the presence of calculus. In addition, seven sites with both P. gingivalis and Bacteroides forsythus showed a correlation with gingival inflammation. In conclusion, the presence of P. gingivalis or P. gingivalis and B. forsythus may be a risk marker to be sought in screening for the onset of periodontal disease.
Suda
Reiko
R
Department of Periodontics, Showa University Dental School, Tokyo, Japan. okiers@aol.com
Kurihara
Chikako
C
Kurihara
Masaki
M
Sato
Taisuke
T
Lai
Chern-Hsiung
CH
Hasegawa
Kohji
K
D016976:Aggregatibacter actinomycetemcomitans / Q000145:classification / Q000302:isolation & purification; D000704:Analysis of Variance; D001439:Bacteroides / Q000145:classification / Q000302:isolation & purification; D015415:Biomarkers; D002167:Campylobacter / Q000302:isolation & purification; D016009:Chi-Square Distribution; D002648:Child; D015169:Colony Count, Microbial; D003728:Dental Calculus / Q000382:microbiology; D003773:Dental Plaque / Q000382:microbiology*; D003774:Dental Plaque Index; D016124:Eikenella corrodens / Q000302:isolation & purification; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000302:isolation & purification; D006801:Humans; D007564:Japan; D008297:Male; D008963:Molar / Q000382:microbiology*; D010518:Periodontitis / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D012307:Risk Factors; D012703:Serotyping; D018709:Statistics, Nonparametric
D015415:Biomarkers