title: A compositional analysis of human nasal septal cartilage.
abstract: BACKGROUND
  Nasal septal cartilage is well established as an autograft material. Tissue engineering methods are now being developed to synthesize cartilage constructs with the properties of this type of cartilage. However, important baseline data on the composition of native septal cartilage is sparse.
  
  
  OBJECTIVE
  To characterize quantitatively the major biochemical constituents of native adult human septal cartilage and determine age- or sex-related variation in composition.
  
  
  METHODS
  Cartilage was harvested from the inferior region of the nasal septum in 33 patients (mean +/- SD age, 47.0 +/- 13.5 years; range, 24-80 years) during routine septoplasty or septorhinoplasty. Biochemical assays were used to determine the quantities, relative to wet weight, of the major constituents of cartilage: water, collagen (from hydroxyproline), sulfated glycosaminoglycan (sGAG), and chondrocytes (from DNA).
  
  
  RESULTS
  On average, each gram of wet cartilage contained 77.7% water, 7.7% collagen, 2.9% sGAG, and 24.9 million cells. Hydration and collagen content showed no significant age variation. Advancing age was associated with a reduction in sGAG content (7.7% per decade, P =.02) and cellularity (7.4% per decade, P =.05). No significant sex differences were found in any of these cartilage constituents.
  
  
  CONCLUSIONS
  This study represents the first biochemical characterization of the composition of native human septal cartilage. The data serve as a baseline for future comparison of the properties of tissue-engineered neocartilage constructs. Furthermore, the age-associated variations in cartilage composition have implications for patient selection for reconstructive procedures.
authors.affiliation: Division of Head and Neck Surgery, Veterans Affairs Hospital, 3350 La Jolla Village Dr, 112C, San Diego, CA 92161, USA.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Homicz
authors.forename: Kevin B
authors.initials: KB
authors.lastname: McGowan
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Lottman
authors.forename: Gordon
authors.initials: G
authors.lastname: Beh
authors.forename: Robert L
authors.initials: RL
authors.lastname: Sah
authors.forename: Deborah
authors.initials: D
authors.lastname: Watson
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D002452:Cell Count; D019902:Chondrocytes / Q000502:physiology; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis; D006801:Humans; D006909:Hydroxyproline / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009300:Nasal Septum / Q000737:chemistry* / Q000166:cytology*; D012737:Sex Factors; D023822:Tissue Engineering; D014867:Water / Q000032:analysis
chemical_list: D006025:Glycosaminoglycans; D014867:Water; C013786:A73025; D006909:Hydroxyproline