Characterization of a putative type IV aminophospholipid transporter P-type ATPase.
The P-type ATPases comprise a well-studied family of proteins involved in the active transport of charged substrates across biological membranes. Starting from a mouse bone marrow-derived macrophage cDNA library and using a signal peptide trapping strategy, we identified a new P-type ATPase family member. We characterized the genomic structure of this gene, named Atp10d, as well as its human counterpart. The presence of P-type ATPase consensus motifs and phylogenetic analysis showed that this gene is a member of the type IV, putative amphipath transporters subfamily. We showed that this gene is expressed in kidney and placenta. We also found that the C57BL/6 strain carries a constitutive stop codon in the sequence of Atp10d exon 12, whereas 14 other inbred mouse strains show an uninterrupted reading frame at this location. This mutation in C57BL/6 should lead to a non-functional protein, suggesting that this gene may not be essential. We discuss the involvement of the Atp10d gene in the fat-prone phenotype of the C57BL/6 strain and its physical mapping within a QTL associated with HDL-cholesterol levels.
Flamant
Stéphane
S
Hôpital Necker-Enfants Malades, INSERM U393, Tour Lavoisier, 2 ème étage, 149 rue de Sèvres, 75015 Paris, France.
Pescher
Pascale
P
Lemercier
Brigitte
B
Clément-Ziza
Mathieu
M
Képès
Franois
F
Fellous
Marc
M
Milon
Geneviève
G
Marchal
Gilles
G
Besmond
Claude
C
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D019295:Computational Biology; D006801:Humans; D008252:Macaca fascicularis; D008264:Macrophages; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D019151:Peptide Library; D049294:Phospholipid Transfer Proteins; D010802:Phylogeny; D021382:Protein Sorting Signals; D017422:Sequence Analysis, DNA
D002352:Carrier Proteins; D008565:Membrane Proteins; D019151:Peptide Library; D049294:Phospholipid Transfer Proteins; D021382:Protein Sorting Signals; D000251:Adenosine Triphosphatases