[1.4 kb 5' flanking region of class I patatin directs tuber-specific gus expression in potato (Solanum tuberosum L.)].
Binary vectors pPATIs (with partial signal sequence) and pPATI (without signal sequence) were constructed by fusing 1.4 kb 5' flanking regions of Class I patatin gene with GUS. Transient GUS expression was observed in in vitro tuber slices bombarded with pPATI. These constructs were then introduced into potato (cv. Desiree) via Agrobacterium tumefaciens transformation. Transgenic potato plants were confirmed by X-Gluc staining and PCR. Using in vitro tuberization system, GUS activities were assayed by fluorescence. It was shown that, in plants transformed with PATI-GUS, GUS specific activities were about 10-20 fold higher in tubers than in stems. Increased sucrose concentration could not induce PATI-GUS expression, but light enhanced PATI-GUS expression in cultured shoots.
Song
D G
DG
State Key Laboratory of Genetics and Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433.
Zhou
J
J
Huang
D Q
DQ
Ma
H
H
Situ
J F
JF
Wang
G Q
GQ
Wang
X M
XM
D002265:Carboxylic Ester Hydrolases / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D005966:Glucuronidase / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011198:Solanum tuberosum / Q000235:genetics*
D010940:Plant Proteins; C043222:patatin protein, Solanum tuberosum; D002265:Carboxylic Ester Hydrolases; D005966:Glucuronidase