UUUUUNU oligonucleotide stimulation of vaccinia virus early gene transcription termination, in trans.
Vaccinia virus early gene transcription termination requires the vaccinia termination factor (VTF), NPH I, a single stranded DNA-dependent ATPase, the virion form of RNA polymerase containing the Rap 94 subunit, and the signal UUUUUNU, which resides in the nascent mRNA, located 30 to 50 bases upstream from the poly(A) addition site. Evidence indicates that a required termination factor acts through binding to the UUUUUNU signal. To further investigate the function of UUUUUNU, the ability of UUUUUNU containing oligonucleotides to inhibit transcription termination was tested. A 22-mer RNA oligonucleotide containing a central U9 sequence exhibited sequence and concentration-dependent stimulation of premature transcription termination and transcript release, in trans. Activation of premature termination required VTF, NPH I, Rap 94, and ATP, demonstrating that the normal termination machinery was employed. Premature termination was not stimulated by RNA harboring a mutant UUUUUNU, demonstrating specificity. These data are consistent with a model in which a required termination factor is converted from an inactive to an active form by binding to a UUUUUNU containing oligonucleotide. The active termination factor then interacts with the ternary complex stimulating transcription termination through the normal mechanism, independent of the nascent mRNA sequence.
Mohamed
Mohamed Ragaa
MR
Department of Microbiology, State University of New York, School of Medicine and Biomedical Sciences, Buffalo, New York 14214, USA.
Niles
Edward G
EG
D017766:Acid Anhydride Hydrolases / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D015967:Gene Expression Regulation, Viral; D043583:Nucleoside-Triphosphatase; D009841:Oligonucleotides / Q000494:pharmacology; D014158:Transcription, Genetic; D014529:Uridine / Q000235:genetics; D014616:Vaccinia virus / Q000235:genetics* / Q000254:growth & development*; D014764:Viral Proteins / Q000235:genetics; D014765:Viral Vaccines; D014779:Virus Replication / Q000235:genetics*
D009841:Oligonucleotides; C085819:RAP94 protein, Vaccinia virus; D014764:Viral Proteins; D014765:Viral Vaccines; D017766:Acid Anhydride Hydrolases; D043583:Nucleoside-Triphosphatase; D014529:Uridine