[Determination of tetracyclines antibiotics by reversed-phase high performance liquid chromatography].
A method for the determination of 7 tetracycline antibiotics (TCs) by reversed-phase high performance liquid chromatography is described. TCs were successfully separated on a Diamonsil C18 column (250 mm x 4.6 mm i.d., 5 microns) using methanol-acetonitrile-0.01 mol/L oxalic acid (pH 2.0) (11:22:67, volume ratio) as mobile phase at a flow rate of 0.8 mL/min and detected at 267 nm within 22 min. Effects of the pH value of the mobile phase, concentration of the mobile phase buffer, elution composition and detection wavelength on the response and retention were studied. Tetracycline (TC) and oxytetracycline(OTC) in medicinal tablets were quantitated by standard added method. It has been proven that the method is fast, accurate and suitable for routine analysis.
Wang
Lei
L
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
Zhang
Xiao-song
XS
Xu
Zhi-xiu
ZX
Shao
Xue-guang
XG
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004318:Doxycycline / Q000032:analysis; D010118:Oxytetracycline / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013752:Tetracycline / Q000032:analysis
D000900:Anti-Bacterial Agents; D013752:Tetracycline; D004318:Doxycycline; D010118:Oxytetracycline