[Application of high performance liquid chromatography to the study on the production of maltooligosaccharide by synergistic action of two-enzymes].
This paper describes the application of high performance liquid chromatograpy to the study on the production of maltooligosaccharide by synergistic action of two-enzymes. Seven sugars in products were separated on a column of Nucleosil C18 with water as the mobile phase and determined refractometrically. Meanwhile, for the preparation of maltooligosaccharide, the effect of supplementing rate of the two enzymes on the content of maltooligosaccharide in products is examined in this paper. The optimun technological conditions were obtained by using the orthogonal design and the amount of the two enzymes added was determined. Thus more than 70% of M3-M6 maltooligosaccharide in product was obtained.
Wu
H
H
Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, 350002.
Tang
G
G
Li
H
H
Li
Z
Z
D002851:Chromatography, High Pressure Liquid; D005524:Food Technology / Q000379:methods; D007517:Isoamylase / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis / Q000138:chemical synthesis*; D000516:alpha-Amylases / Q000032:analysis*
D009844:Oligosaccharides; C021705:maltooligosaccharides; D000516:alpha-Amylases; D007517:Isoamylase