Ligand-receptor interactions: a new method for determining the binding parameters.
A new experimental procedure and new plot coordinates that allow determination of the binding parameters of ligand-acceptor interaction have been proposed. Instead of titration of a constant concentration of receptors with changing concentrations of ligand, as requested by the well-known methods of Klotz and Scatchard, a series of sequential dilutions of the reacting ligand-receptor mixture is suggested. This allows the application of a new coordinate system that transforms the binding isotherms into straight lines. The case of one acceptor with two classes of receptors with different binding constants is also considered briefly, where the correspondent graphs are nonlinear. It is suggested that in some cases this approach can be a simple and convenient substitute of the broadly used methods of Klotz and Scatchard.
Bobrovnik
S A
SA
Department of Molecular Immunology, Palladin Institute of Biochemistry, Kiev 01030, Ukraine. sab@biochem.kiev.ua
D001665:Binding Sites; D001703:Biophysics / Q000379:methods*; D007700:Kinetics; D008024:Ligands; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000378:metabolism; D013696:Temperature
D008024:Ligands