[Studies on the separation and determination of natural phenolic acids by reversed-phase high performance liquid chromatography].
A new method using gradient elution of reversed-phase high performance liquid chromatography was set up for the separation and determination of 12 natural phenolic acids simultaneously. The separation conditions were studied with a Zorbax ODS (5 microns) column, and optimized conditions were suggested with the mobile phase gradient elution of CH3CN-H2O (with 0.5% HAc) (5:95-->35:65, V/V, in 40 min) at room temperature, flow rate at 1.0 mL/min and detection wavelength at 280 nm. And the effects of acidity on separations and the relationships between the phenolic acids structures and their retention behaviors were discussed. Qualitative and quantitative analysis of the 12 compounds were performed. The regression equation, correlation coefficient, linear range, detection limit and relative standard deviation (RSD) of each compound were obtained under the optimized conditions. The correlation coefficients of the 12 compounds were between 0.9980 and 0.9999. The detection limits ranged from 0.96 ng to 4.10 ng and RSDs were < or = 2.64%. These results demonstrate that the optimized determination method obtained is successful for the separation and determination of the 12 phenolic acids analysed.
Liu
Jiang-yun
JY
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100094, China. jiangyunliu@263.net
Yang
Xue-dong
XD
Xu
Li-zhen
LZ
Yang
Shi-lin
SL
D019817:Benzoic Acid / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000032:analysis / Q000302:isolation & purification; D003373:Coumaric Acids / Q000032:analysis / Q000302:isolation & purification; D005707:Gallic Acid / Q000032:analysis / Q000302:isolation & purification; D062385:Hydroxybenzoates / Q000032:analysis / Q000302:isolation & purification*
D002934:Cinnamates; D003373:Coumaric Acids; D062385:Hydroxybenzoates; C029010:cinnamic acid; D005707:Gallic Acid; D019817:Benzoic Acid; C004999:ferulic acid; C017616:phenolic acid