A rapid method for RNA preparation from Gram-positive bacteria.
We have developed a rapid method for preparation of RNA from various Gram-positive bacteria. Unlike some methods, this method does not require lysozyme and proteinase K. Instead, we used glass beads to break cells more efficiently. Using this method, we successfully isolated the total RNA from various Gram-positive bacteria. This method is rapid, simple, and more economic when compared to the previously reported ones. Prepared RNA can be used for the transcriptional analysis of various Gram-positive bacteria.
Oh
Eun Taex
ET
Department of Biological Engineering and Center for Advanced Bioseparation Technology, Inha University, Incheon 402-751, South Korea.
So
Jae-Seong
JS
D004587:Electrophoresis, Agar Gel / Q000379:methods; D016680:Genome, Bacterial; D006094:Gram-Positive Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008863:Microspheres; D019800:Phenol / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013997:Time Factors
D012329:RNA, Bacterial; D019800:Phenol