[Determination of dyes in cosmetic by high performance liquid chromatography].
A reversed-phase high performance liquid chromatographic method was used to detect 5 dyes including p-phenylenediamine in cosmetrics. A Zorbax C8 column was used and mobile phase was V (triethanolamine):V(water):V(acetonitrile) = 0.95:94.05:5 adjusted to pH 7.7 by phosphoric acid. The wavelength 280 nm was selected. Samples were extracted with 95% ethanol by ultrasonic method. The recoveries were 87%-107% and the relative standard deviations were 2.3%-6.4%.
Wang
J
J
Institute of Environmental Health Monitoring, Chinese Academy of Preventive Medicine, Beijing 100021, China.
Hu
J
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004396:Coloring Agents / Q000032:analysis*; D006202:Hair Dyes / Q000032:analysis*; D006873:Hydroquinones / Q000032:analysis; D010655:Phenylenediamines / Q000032:analysis*
D004396:Coloring Agents; D006202:Hair Dyes; D006873:Hydroquinones; D010655:Phenylenediamines; C029728:4-phenylenediamine; C031927:hydroquinone