title: Relation of aging with oxidative protein damage parameters in the rat skeletal muscle.
abstract: OBJECTIVE
  An increase in oxidative stress may contribute to the development of oxidative protein damage in the aging rat skeletal muscle. Our aim was to reveal protein carbonyl (PCO), advanced oxidation protein products (AOPP), a novel marker of oxidative stress, and protein thiol (P-SH) levels as markers of protein oxidation, as well as lipid hydroperoxide (LHP) levels as a marker of lipid peroxidation, and relation of nitrotyrosine (NT) levels with these markers in skeletal muscle tissue of young, adult, and old male Wistar rats.
  
  
  METHODS
  In the present study, we investigated the relation between aging and oxidative protein damage parameters such as PCO, NT, AOPP, and P-SH, as well as oxidative stress parameters such as total thiol, nonprotein thiol, and LHP in the skeletal muscle tissue of young, adult, and old Wistar rats.
  
  
  RESULTS
  PCO and NT levels of old rats were significantly increased compared with those of young and adult rats. Skeletal muscle AOPP levels were significantly increased in old rats compared with those of adult rats. P-SH levels were significantly decreased in old rats compared with those of young rats.
  
  
  CONCLUSIONS
  The finding that the increase in PCO levels of young vs. old group was more significant than that of adult vs. old group may suggest that PCO formation is an early specific marker of aging process in skeletal muscle. In addition, increased levels of nitrotyrosine in the skeletal muscle of the old rat group may be a novel specific marker of oxidative protein damage in the aging muscle. The absence of correlation between oxidative protein damage markers mentioned above and LHP levels may indicate that protein oxidation and lipid peroxidation in the aging rat skeletal tissue are two distinct mechanisms.
authors.affiliation: Istanbul University, Istanbul Faculty of Medicine, Central Laboratory of Biochemistry, Capa 34390, Istanbul, Turkey.
authors.forename: Ufuk
authors.initials: U
authors.lastname: Cakatay
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Telci
authors.forename: Refik
authors.initials: R
authors.lastname: Kayali
authors.forename: Fatma
authors.initials: F
authors.lastname: Tekeli
authors.forename: Tülay
authors.initials: T
authors.lastname: Akçay
authors.forename: Ahmet
authors.initials: A
authors.lastname: Sivas
mesh_terms: D000375:Aging; D000818:Animals; D002244:Carbon / Q000097:blood; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000097:blood; D013997:Time Factors; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism
chemical_list: D008054:Lipid Peroxides; D013438:Sulfhydryl Compounds; C002744:3-nitrotyrosine; D014443:Tyrosine; D002244:Carbon; D010100:Oxygen