Role of anthocyanidin reductase, encoded by BANYULS in plant flavonoid biosynthesis.
Condensed tannins (CTs) are flavonoid oligomers, many of which have beneficial effects on animal and human health. The flavanol (-)-epicatechin is a component of many CTs and contributes to flavor and astringency in tea and wine. We show that the BANYULS (BAN) genes from Arabidopsis thaliana and Medicago truncatula encode anthocyanidin reductase, which converts anthocyanidins to their corresponding 2,3-cis-flavan-3-ols. Ectopic expression of BAN in tobacco flower petals and Arabidopsis leaves results in loss of anthocyanins and accumulation of CTs.
Xie
De-Yu
DY
Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.
Sharma
Shashi B
SB
Paiva
Nancy L
NL
Ferreira
Daneel
D
Dixon
Richard A
RA
D000595:Amino Acid Sequence; D000872:Anthocyanins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins; D002392:Catechin / Q000378:metabolism; D002942:Circular Dichroism; D017343:Genes, Plant; D009682:Magnetic Resonance Spectroscopy; D029902:Medicago / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000378:metabolism; D030821:Plants, Genetically Modified; D044945:Proanthocyanidins; D011994:Recombinant Proteins / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D013237:Stereoisomerism; D013634:Tannins / Q000096:biosynthesis* / Q000737:chemistry; D014026:Nicotiana / Q000235:genetics
D000872:Anthocyanins; D029681:Arabidopsis Proteins; D044945:Proanthocyanidins; D011994:Recombinant Proteins; D013634:Tannins; D009243:NAD; C013221:proanthocyanidin; D009249:NADP; D002392:Catechin; D010105:Oxygenases; C118159:anthocyanidin synthase; C401411:BANYULS protein, Arabidopsis; D009247:NADH, NADPH Oxidoreductases