title: [The optimization of guanosine fermentation based on process parameter correlation analysis].
abstract: The characteristic of Bacillus subtilis fermentation process of guanosine on 50 L fermentor was analyzed. Based on determination of on-line and off-line parameter, using correlation analysis, the technology study of physiologic regulation was combined with the metabolic flux distribution of synthesis process. The metabolic flux shift from HMP to EMP and TCA cycle during fermentation was found. The reason of the flux shift was preliminary analyzed, based on which the procedure was optimized to increase the yield of guanosine to 30 g/L.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, ECUST, Shanghai 2000237, China.
authors.forename: Xianpeng
authors.initials: X
authors.lastname: Cai
authors.forename: Shuangxi
authors.initials: S
authors.lastname: Chen
authors.forename: Ju
authors.initials: J
authors.lastname: Chu
authors.forename: Yingping
authors.initials: Y
authors.lastname: Zhuang
authors.forename: Siliang
authors.initials: S
authors.lastname: Zhang
authors.forename: Huanzhang
authors.initials: H
authors.lastname: Wang
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001412:Bacillus subtilis / Q000378:metabolism*; D019149:Bioreactors; D002245:Carbon Dioxide / Q000378:metabolism; D005285:Fermentation; D006151:Guanosine / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism
chemical_list: D006151:Guanosine; D002245:Carbon Dioxide; D000641:Ammonia; D010100:Oxygen