[Activation of G protein on the membrane of TCS-sensitive cells].
Trichosanthin (TCS), a Type I Ribosome Inactivating Protein isolated from the root tuber of Trichosanthes Kirilowii M. has various biological activities including abortion induction, anti-tumor and anti-HIV. The mechanism of TCS specifically killing sensitive cells has not been studied clearly. In this study, we initially found that there exists TCS-affinity molecule on Syncytiotrophoblast cells and Jar cells. Furthermore, by [35S]GTP gamma S Binding Assay, we find that TCS can activate G protein on the membrane of TCS-sensitive cells. These results indicate that on the membrane of TCS-sensitive cells exists TCS-specific receptor.
Wu
Z H
ZH
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031.
Nie
H L
HL
Lu
B
B
Wang
Q
Q
Ke
Y B
YB
D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D019204:GTP-Binding Proteins / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D020014:K562 Cells; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015978:Trichosanthin / Q000494:pharmacology*; D014327:Trophoblasts / Q000166:cytology
D011956:Receptors, Cell Surface; D015978:Trichosanthin; D019204:GTP-Binding Proteins