[Determination of phenazine-1-carboxylic acid in anti-fungal agent M18 by high performance liquid chromatography].
A reversed-phase HPLC method for the determination of phenazine-1-carboxylic acid (PCA) in antifungal agent M18 is established. The mobile phase was a mixture of MeOH-5 mmol/L phosphate buffer (pH 5.0) (60:40, volume ratio). The flow rate was 1.0 mL/min, and the detection wavelength was 248 nm. The linear range and detectable limit were 50 mg/L-500 mg/L and 30 mg/L respectively. The recovery was 97.53% and RSD was 1.5%. The method of PCA extraction and detection has proven to be much faster, simpler, more sensitive, accurate and reproducible than those reported already. The assay results can be used as a very important criterion for large-scale production.
Zhu
D H
DH
College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China.
Zhu
X D
XD
Xu
Y Q
YQ
D000935:Antifungal Agents / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D010619:Phenazines / Q000032:analysis*; D015203:Reproducibility of Results
D000935:Antifungal Agents; D010619:Phenazines; C037165:1-phenazinecarboxylic acid