[The millipore rapid screening of hydantoinase production strains].
OBJECTIVE
To find a strain with a higher hydantoinase activity.


METHODS
The millipore rapid screening method was established to select the strains. The effects of several solvents on the rate of biotransformation were compared.


RESULTS
A hydantoinase production strain has been selected by the millipore rapid screening method. Tween-80 (1%) is found to be a suitable solvent to dissolve the substrate.


CONCLUSIONS
A new method is provided for rapid selection of hydantoinase production strains from large-scale samples. The difficulty in dissolving the substrate has been resolved to some extent.
Luo
X
X
Department of Biochemistry, School of Basic Medical Sciences, WCUMS, Chengdu 610041, China.
Feng
R
R
Hu
Z
Z
D000581:Amidohydrolases / Q000096:biosynthesis* / Q000378:metabolism; D001711:Biotransformation; D011136:Polysorbates; D011549:Pseudomonas / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*
D011136:Polysorbates; D000581:Amidohydrolases; C020047:dihydropyrimidinase