[Study on the interaction between the 5' proximal region of mGAT-1 and nuclear proteins by the method of SPR].
The DNA fragment (named F182) corresponding the position of -1775(-)-1594 in the mouse GABA transporter 1 (mGAT-1) 5' proximal region was amplified by PCR. Then the DNA was immobilized to the surface of sensor chip SA5 via biotin-streptavidin linkage. The interaction between the F182 on SA5 and nuclear proteins from mouse liver and kidney was studied by the method of SPR with Biosensor of BIAcore-1000 respectively. The Binding between F182 and two nuclear proteins was definitely and specifically and both with the apparent dissociation rate of about 1.4E-5/s. Competitive experiment revealed that a conserved sequence within F182 had the main contribution to the binding event.
Wang
Q
Q
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
Cai
G Q
GQ
Fei
J
J
Zhou
M Y
MY
Guo
L H
LH
D000217:Acyltransferases / Q000235:genetics*; D000818:Animals; D015374:Biosensing Techniques; D002352:Carrier Proteins / Q000235:genetics*; D004247:DNA / Q000378:metabolism*; D050485:GABA Plasma Membrane Transport Proteins; D008565:Membrane Proteins / Q000235:genetics*; D026901:Membrane Transport Proteins; D051379:Mice; D017351:N-Acetylglucosaminyltransferases; D009687:Nuclear Proteins / Q000378:metabolism*; D027361:Organic Anion Transporters; D016133:Polymerase Chain Reaction; D011485:Protein Binding
D002352:Carrier Proteins; D050485:GABA Plasma Membrane Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009687:Nuclear Proteins; D027361:Organic Anion Transporters; D004247:DNA; D000217:Acyltransferases; C459903:MGAT1 protein, mouse; D017351:N-Acetylglucosaminyltransferases