Streptomyces and Escherichia coli, model organisms for the analysis of the initiation of bacterial chromosome replication.
Streptomyces coelicolor A3(2) and Escherichia coli are quite different in their life-style and the structures of their genomes. Streptomyces exhibit complex multicellular development including formation of multigenomic hyphae during growth. These organisms possess a large linear (8.7 Mb) and GC-rich (approximately 72%) chromosome. The genome sequence of S. coelicolor has just been completed. The difference between E. coli and Streptomyces making them an excellent model organisms for a comparison of their replication modes. In this review, we compare initiation of chromosome replication in both organisms. Their replication origins are different in size, but both have DnaA boxes--a binding motifs for initiator DnaA protein. The two DnaA proteins have practically the same biochemical properties. Many aspects of the control of initiation seem to be similar. A comparison of the two systems thus allows us to define those aspects of replication initiation that are universally used in the eubacterial kingdom.
Messer
Walter
W
Max-Planck-Institut für molekulare Genetik, D-14195 Berlin, Germany. messer@molgen.mpg.de
Zakrzewska-Czerwińska
Jolanta
J
D001426:Bacterial Proteins / Q000378:metabolism; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D018741:Replication Origin; D013302:Streptomyces / Q000235:genetics*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria