Isolation, chemical investigation and antiviral activity of polysaccharides from Gracilaria corticata (Gracilariaceae, Rhodophyta).
Polysaccharides were sequentially extracted from the agarophyte Gracilaria corticata. Chemical analysis combined with infrared spectroscopy showed that the cold water extracted material consists mainly of a high molecular weight sulfated galactan. Most of the sulfate groups are alkali labile and are located at C-4 of the 1,3-linked D-galactose units and C-6 of the 1,4-linked L-galactose residues. The autoclaved extracts contain agar type polysaccharide having a high pyruvate content and a variable degree of methylation, but were contaminated with floridean starch. 1H-NMR studies indicate that methoxyl groups, when present, occur at C-6 of the 1,3-linked D-galactose units and C-2 of the 1,4-linked L-galactose residues of agar polymer. Bioassays showed that a high molecular weight galactan sulfate, exhibited selective antiviral activity against herpes simplex virus types 1 and 2, likely due to an inhibition of the initial virus attachment to the host cell.
Mazumder
Sutapa
S
Department of Chemistry, The University of Burdwan, 713 104, WB, Burdwan, India.
Ghosal
Prodyut K
PK
Pujol
Carlos A
CA
Carlucci
María J
MJ
Damonte
Elsa B
EB
Ray
Bimalendu
B
D000362:Agar / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D001681:Biological Assay; D002522:Chlorocebus aethiops; D002855:Chromatography, Thin Layer; D004305:Dose-Response Relationship, Drug; D005685:Galactans / Q000737:chemistry; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D011134:Polysaccharides / Q000737:chemistry*; D018139:Simplexvirus / Q000378:metabolism; D013055:Spectrophotometry, Infrared; D013213:Starch / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry / Q000494:pharmacology; D014709:Vero Cells
D000925:Anticoagulants; D000998:Antiviral Agents; D005685:Galactans; D011134:Polysaccharides; D013431:Sulfates; D000362:Agar; D013213:Starch