title: [Detection of hypodermal and hematopoietic necrosis baculovirus by PCR method].
abstract: For control of the explosive epidemic disease of Penaeid shrimp in China. We have developed a method for detection of Hypodermal and hematopoietic necrosis baculovirus (HHNBV) by polymerase chain reaction (PCR). Two pairs of PCR primers (DP1/DP2 and DP3/DP4) were prepared, based on the RFLP analysis between HHNBV and penaeid rod-shaped DNA virus. PCR using either DP1/DP2 or DP3/DP4 specified HHNBV genome. When PCR products amplified with DP1/DP2 were subjected to nested PCR with DP3/DP4, a high sensitivity about 0.04 pg was seem with the nested PCR.
authors.affiliation: Department to Microbiology an Infection, College of Velerinary Medicine, China Agricultural University Beijing 100094.
authors.forename: C
authors.initials: C
authors.lastname: Xia
authors.forename: J
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D033364:Decapoda / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral