In situ imaging of specific binding of [3H]isatin in rat brain.
Isatin is an endogenous indole that influences a range of processes both in vivo and in vitro. It has a distinct and discontinuous distribution in the brain, as well as in other mammalian tissues and body fluids. However, the distribution of isatin binding sites in the brain is not known. Using a real-time beta-imager we have investigated the distribution of [3H]isatin-specific binding in rat brain sections. The highest labeling was found in hypothalamic nuclei and in the cortex, hippocampus, and cerebellum. Administration of the mechanism based monoamine oxidase inhibitor, pargyline, reduced but did not abolish the specific binding of [3H]isatin in the rat brain. The distribution became cortex, cerebellum, hypothalamus > hippocampus > brain stem > thalamus approximately striatum.
Crumeyrolle-Arias
Michèle
M
Laboratoire CRRET -CNRS FRE2412, Université Paris 12, Créteil, France. crumeyrolle@univ-paris12.fr
Medvedev
Alexei
A
Cardona
Ana
A
Barritault
Denis
D
Glover
Vivette
V
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites / Q000187:drug effects; D001667:Binding, Competitive / Q000187:drug effects; D001921:Brain / Q000000981:diagnostic imaging / Q000187:drug effects / Q000378:metabolism*; D007510:Isatin / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D010293:Pargyline / Q000494:pharmacology; D011877:Radionuclide Imaging; D051381:Rats; D017208:Rats, Wistar; D014316:Tritium
D008996:Monoamine Oxidase Inhibitors; D014316:Tritium; D007510:Isatin; D010293:Pargyline