title: Rapid in vivo screening of experimental drugs for tuberculosis using gamma interferon gene-disrupted mice.
abstract: We have developed a rapid new in vivo method for screening experimental drugs for their activity against Mycobacterium tuberculosis by using the gamma interferon gene-disrupted (GKO) C57BL/6 mouse. Due to the rapid growth of the infection, statistical differences indicating positive efficacy of active compounds can be seen after only 8 days of treatment. To validate this model, several fluoroquinolones, including ciprofloxacin, levofloxacin, moxifloxacin, and gatifloxacin, were tested in parallel.
authors.affiliation: Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA. lenaerts@colostate.edu
authors.forename: Anne J M
authors.initials: AJ
authors.lastname: Lenaerts
authors.forename: Veronica
authors.initials: V
authors.lastname: Gruppo
authors.forename: Jason V
authors.initials: JV
authors.lastname: Brooks
authors.forename: Ian M
authors.initials: IM
authors.lastname: Orme
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D024841:Fluoroquinolones; D007371:Interferon-gamma; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis; D014376:Tuberculosis / Q000188:drug therapy*
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D007371:Interferon-gamma