[Determination of lamotrigine in the serum and study on pharmacokinetics in rabbits by RP-HPLC].
In the treatment of epileptic patients a method was developed to monitor the lamotrigine concentration by HPLC using a YWG-ODS column and a detector at 305 nm. The linear range was from 0.25 mg/L to 50 mg/L with a mean correlation coefficient of 0.9998. The average recovery was 93.78% and the standard deviation was 2.66%. Within and between-day precisions were 2.4% (n = 9) and 5.3% (n = 5) in RSD respectively. The pharmacokinetics was studied after a single dose of 25 mg/kg for 3 rabbits. Lamotrigine concentrations in serum were assayed and the parameters were computed with 3P87 program. The concentration-time curve of lamotrigine in rabbits fitted to a two compartment model, in which Cmax was 9.34 micrograms/mL, t1/2(Ke) was 8.78 h, and CL/f(s) was 0.69 L/h. The method is rapid, accurate and can be used as routine test in the use of lamotrigine.
Hu
Y S
YS
175th Hospital PLA, Zhangzhou 363000, China.
Wu
P
P
Tang
Q H
QH
D000818:Animals; D000927:Anticonvulsants / Q000097:blood / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D000077213:Lamotrigine; D008297:Male; D011817:Rabbits; D014227:Triazines / Q000097:blood / Q000493:pharmacokinetics*
D000927:Anticonvulsants; D014227:Triazines; D000077213:Lamotrigine