title: Characteristics and association with disease of two major subclones of Shiga toxin (Verocytotoxin)-producing strains of Escherichia coli (STEC) O157 that are present among isolates from patients in Germany.
abstract: Shiga toxin (Verocytotoxin) producing E. coli (STEC) O157 were isolated from 168 patients living in different parts of Germany. Most isolates were from sporadic cases and seven small outbreaks with STEC O157 were identified. The 168 strains were examined for phenotypic and genotypical traits in order to identify major types of STEC O157 occurring in Germany. Phage typing (PT) revealed PT8 (n = 54) and PT2 (n = 48) strains as most frequent (60.7%) among the isolates. Carriage of the stx(2) gene by STEC O157 was closely associated with hemolytic uremic syndrome (100%) and with bloody diarrhea (61.7%). The stx(2) gene was frequent in PT88, PT47 (both 100%), PT2 (91.5%) and PT4 (87.5%) strains and more rarely (33.3%) found in strains belonging to the other PTs. PT8 and PT2 strains formed two groups which differed from each other in their motility, stx-genotypes and the severity of the illness they caused. Pulsed-field gel electrophoresis of PT2 and PT8 strains and hybridization of XbaI digested DNA with stx(1) and stx(2) specific gene probes revealed similarities among epidemiologically unrelated strains belonging to the same PT. The results indicate that STEC O157 PT2 and PT8 strains form two distinct subclones which are dominating in Germany and other European countries.
authors.affiliation: Division of Emerging Bacterial Pathogens, Robert Koch-Institut, Berlin, Germany. BeutinL@rki.de
authors.forename: Lothar
authors.initials: L
authors.lastname: Beutin
authors.forename: Stefan
authors.initials: S
authors.lastname: Kaulfuss
authors.forename: Thomas
authors.initials: T
authors.lastname: Cheasty
authors.forename: Börries
authors.initials: B
authors.lastname: Brandenburg
authors.forename: Sonja
authors.initials: S
authors.lastname: Zimmermann
authors.forename: Kerstin
authors.initials: K
authors.lastname: Gleier
authors.forename: Geraldine A
authors.initials: GA
authors.lastname: Willshaw
authors.forename: Henry R
authors.initials: HR
authors.lastname: Smith
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D002648:Child; D002675:Child, Preschool; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000472:pathogenicity*; D005260:Female; D005858:Germany / Q000453:epidemiology; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D010802:Phylogeny; D022621:Shiga Toxin / Q000096:biosynthesis*; D014774:Virulence
chemical_list: D015415:Biomarkers; D022621:Shiga Toxin