Ginseng and aluminium hydroxide act synergistically as vaccine adjuvants.
The dry extract prepared from the Panax ginseng C.A. Meyer-root (total ginseng (T-ginseng)) contain ginsenosides (G-des) which were shown to have adjuvant properties as demonstrated by: (a) injecting guinea pigs with a mixture of T-ginseng and inactivated porcine parvovirus (PPV) as a conventional vaccine; (b)injecting PPV-antigen and T-ginseng simultaneously but separately at different sites on the animal and (c)injecting only the T-ginseng 1 or 2 weeks prior to immunisation with the PPV-antigen. Using a haemagglutination inhibition (HI) test in the antibody titration, it was found that the mean HI-titre for the animals injected with PPV-antigen only was 320 +/- 0. By comparison, the mean titre value was 2026 +/- 1206 for the sera from the animals injected with the same vaccine but adjuvanted with 4 mg of T-ginseng, while the antibody titre induced by a vaccine containing Al(OH)(3)-gel was 2986 +/- 1596. Interestingly, the T-ginseng and Al(OH)(3) acted synergistically and further improved the antibody response to the PPV-antigen to 6826 +/- 2413, i.e. more than 20 times the HI titre of the non-adjuvanted PPV-vaccine. Immunisations using PPV-vaccines adjuvanted with single purified G-des demonstrated that the ginseng fractions Rb1 and Rg1 are potent adjuvants inducing higher or similar antibody titres than the vaccine adjuvanted with Al(OH)(3), e.g. Rb1 tested at a concentration of 830 microg per dose induced a significantly (P = 0.009) higher antibody titre than the one adjuvanted with Al(OH)(3). Nevertheless, different than the mixture Al(OH)(3)-T-ginseng; Rb1 and Rg1 act antagonistically and partially inhibit each other. The G-des adjuvanted vaccines induced significantly (P = 0.0011) higher titres of IgG2 antibodies compared with IgG1.
Rivera
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National Veterinary Institute, Uppsala S-751 89, Sweden. esteban.rivera@sva.se
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D000276:Adjuvants, Immunologic; D000284:Administration, Oral; D000536:Aluminum Hydroxide / Q000008:administration & dosage / Q000276:immunology*; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002417:Cattle; D002645:Chickens; D004306:Dose-Response Relationship, Immunologic; D004357:Drug Synergism; D036145:Ginsenosides / Q000008:administration & dosage / Q000276:immunology*; D006168:Guinea Pigs; D006461:Hemolysis / Q000187:drug effects; D007115:Immunization Schedule; D007117:Immunization, Secondary; D007273:Injections, Intramuscular; D005894:Panax / Q000276:immunology*; D028323:Parvovirus, Porcine / Q000276:immunology*; D010936:Plant Extracts / Q000008:administration & dosage / Q000276:immunology*; D012449:Safety; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D014611:Vaccination; D014765:Viral Vaccines / Q000276:immunology
D000276:Adjuvants, Immunologic; D000956:Antigens, Viral; D036145:Ginsenosides; D010936:Plant Extracts; D014765:Viral Vaccines; D000536:Aluminum Hydroxide