[Screening strains producing inulinase and cloning of inulinase gene].
A wild-type strain AF10 producing inulinase was screened from soil samples, the strain is identified to be a A. niger. A endoinulinase gene inuA1 from A. niger AF10 was wequenced and analyzed, after PCR amplification. The results shows inuA1 is 1551 bp in length without any intron and contains 4 potential N-glycosylation sites. The conservative sequence is WMNEPN from the N-terminus. The inuA1 was cloned to pUC118 and the recombinant vector pinuA1 was obtained and transformed into E. coli JM109. The recombinant JM109/inuA1 included inuA1 was obtained.
Zhang
Linghua
L
Department of Food Science and Bioengineering, Dalian Institute of Light Idustry, Dalian 116001, China.
Wang
Yunji
Y
Ye
Shuhong
S
Zhang
Fuqi
F
D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics* / Q000302:isolation & purification; D005800:Genes, Fungal; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000302:isolation & purification; D010407:Penicillium / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D014170:Transformation, Genetic
D004271:DNA, Fungal; D005656:Fungal Proteins; D006026:Glycoside Hydrolases; C023086:inulinase