title: Crystallization of membrane proteins from media composed of connected-bilayer gels.
abstract: The use of hydrated-lipid gels in which the bilayer is an infinitely periodic (or at least continuous), three-dimensional structure offers a relatively new approach for the crystallization of membrane proteins. While excellent crystals of the Halobacterial rhodopsins have been obtained with such media, success remains poor in extending their use to other membrane proteins. Experience with crystallization of bacteriorhodopsin has led us to recognize a number of improvements that can be made in the use of such hydrated-gel media, which may now prove to be of general value for the crystallization of other membrane proteins.
authors.affiliation: Life Sciences Division, Donner Laboratory, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA.
authors.forename: Shahab
authors.initials: S
authors.lastname: Rouhani
authors.forename: Marc T
authors.initials: MT
authors.lastname: Facciotti
authors.forename: Glenna
authors.initials: G
authors.lastname: Woodcock
authors.forename: Vincent
authors.initials: V
authors.lastname: Cheung
authors.forename: Christian
authors.initials: C
authors.lastname: Cunningham
authors.forename: Doris
authors.initials: D
authors.lastname: Nguyen
authors.forename: Behzad
authors.initials: B
authors.lastname: Rad
authors.forename: Chiann-Tso
authors.initials: CT
authors.lastname: Lin
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Lunde
authors.forename: Robert M
authors.initials: RM
authors.lastname: Glaeser
mesh_terms: D001436:Bacteriorhodopsins / Q000737:chemistry / Q000648:ultrastructure; D003460:Crystallization; D018360:Crystallography, X-Ray; D003470:Culture Media; D005782:Gels; D006217:Halobacterium; D008051:Lipid Bilayers / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D008958:Models, Molecular; D008968:Molecular Conformation; D011487:Protein Conformation; D013816:Thermodynamics
chemical_list: D003470:Culture Media; D005782:Gels; D008051:Lipid Bilayers; D008563:Membrane Lipids; D008565:Membrane Proteins; D001436:Bacteriorhodopsins