[The immunohistochemistry and clinic study on surgical margin of laryngeal cancer].
OBJECTIVE
To probe into the safe incisal range of removing laryngectomy cancer determined from the albumen balance of cells.


METHODS
LSAB or SABC of immunohistochemistry was applied, and three groups of samples with cancer tissue, nearby cancer tissue and distal cancer tissue of 36 primary laryngectomy cancer were tested by antibodies p16, p21, TGF-beta 1, PCNA and EGFR.


RESULTS
The difference of the positive rate with the five antibodies between the cancer group and the two side incisal group were very notable with P < 0.01 or P < 0.05, but which between nearby cancer tissue and distal cancer tissue were not notable with P > 0.05. The differences of the positive rates with 5 antibodied between high differentiation group and low differentiation group were notable with P < 0.05, and P < 0.05 with PCNA in the group whether the lymphonodi cervicales transmitted or not. The incisals for all the cases are beyond 5 mm, the recurerence rate is 3%.


CONCLUSIONS
The 5 mm incisal for laryngectomy cancer is appropriate on the albumen cevel of cells.
Dai
S
S
Department of Otolaryngology, PLA 463 Hospital, Shenyang 110042.
Sun
X
X
Wu
X
X
Zhao
J
J
D000328:Adult; D000368:Aged; D066246:ErbB Receptors / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000737:chemistry / Q000473:pathology / Q000601:surgery*; D007825:Laryngectomy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D016212:Transforming Growth Factor beta / Q000032:analysis
D018809:Proliferating Cell Nuclear Antigen; D016212:Transforming Growth Factor beta; D066246:ErbB Receptors