[Infection of mutated mouse complement receptor type II by Epstein-Barr virus].
Site-directed mutagenesis method was used to introduce two desired mutations, which were confirmed by DNA sequencing, into mouse complement receptor Type II gene(MCR2). Then the constructed eukaryotic expression vectors containing wild type mouse CR2/1(wtMCR2/1), mutant type mouse CR2/1 (mtMCR2/1) and human CR2 (hCR2) cDNA were transferred into mouse SP2/0 cells by electroporation. After two-week screening by G418, the stably transfected clones were obtained. Several ways including PCR, RT-PCR, and immunohistochemistry were utilized to screen those clones with interesting genes integrated and expressed. Then Epstein-Barr virus(EBV) was used to infect these transfected cells and EBER-1 (EBV encoded RNAs) hybridization results showed that only hCR2 and mtMCR2 transfected SP2/0 cells could be infected by EBV, but positive rate of the former was much higher than the latter. This study sets groundwork for elucidating the mechanism by which EBV enters the cells and for establishing the animal model of EBV-related nasopharyngeal carcinoma (NPC).
Ren
C P
CP
Cancer Research Institute, Xiangya Medical College, Central South University, Changsha 410078, China.
Lan
K
K
Liu
W D
WD
He
Z W
ZW
Wang
H
H
Yao
K T
KT
D000818:Animals; D018274:Electroporation; D020031:Epstein-Barr Virus Infections; D005057:Eukaryotic Cells / Q000378:metabolism / Q000821:virology; D018014:Gene Transfer Techniques; D004854:Herpesvirus 4, Human; D006801:Humans; D051379:Mice; D009303:Nasopharyngeal Neoplasms / Q000821:virology; D017354:Point Mutation; D017464:Receptors, Complement 3d / Q000235:genetics*; D014162:Transfection
D017464:Receptors, Complement 3d