title: Serum antibodies to Dicrocoelium dendriticum in sheep from Sardinia (Italy).
abstract: An indirect enzyme-linked immunosorbent assay (ELISA) with Dicrocoelium dendriticum excretory/secretory antigens was used to evaluate the presence of serum antibodies against the trematode in 738 sheep randomly chosen in Sardinia (Italy). Coprological sedimentation was used to discover egg-output. Seropositivity was detected in 86.2% tested sheep, whereas faecal prevalence was 6.7%; all that were faecal-positive also were ELISA-positive.
authors.affiliation: Departamento de Patologi;a Animal, Parasitologi;a y Enfermedades Parasitarias, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27071 Lugo, Spain. ritasan@lugo.usc.es
authors.forename: R
authors.initials: R
authors.lastname: Sánchez-Andrade
authors.forename: A
authors.initials: A
authors.lastname: Paz-Silva
authors.forename: J L
authors.initials: JL
authors.lastname: Suárez
authors.forename: M
authors.initials: M
authors.lastname: Arias
authors.forename: C
authors.initials: C
authors.lastname: López
authors.forename: P
authors.initials: P
authors.lastname: Morrondo
authors.forename: A
authors.initials: A
authors.lastname: Scala
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000302:isolation & purification*; D000947:Antigens, Helminth / Q000276:immunology; D004011:Dicrocoeliasis / Q000175:diagnosis / Q000276:immunology* / Q000469:parasitology / Q000662:veterinary*; D004013:Dicrocoelium / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D005260:Female; D007558:Italy; D008297:Male; D010270:Parasite Egg Count; D015995:Prevalence; D012680:Sensitivity and Specificity; D012757:Sheep Diseases / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology; D034561:Sheep, Domestic / Q000276:immunology* / Q000469:parasitology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth