title: Reevaluating human gene annotation: a second-generation analysis of chromosome 22.
abstract: We report a second-generation gene annotation of human chromosome 22. Using expressed sequence databases, comparative sequence analysis, and experimental verification, we have extended genes, fused previously fragmented structures, and identified new genes. The total length in exons of annotation was increased by 74% over our previously published annotation and includes 546 protein-coding genes and 234 pseudogenes. Thirty-two potential protein-coding annotations are partial copies of other genes, and may represent duplications on an evolutionary path to change or loss of function. We also identified 31 non-protein-coding transcripts, including 16 possible antisense RNAs. By extrapolation, we estimate the human genome contains 29,000-36,000 protein-coding genes, 21,300 pseudogenes, and 1500 antisense RNAs. We suggest that our revised annotation criteria provide a paradigm for future annotation of the human genome.
authors.affiliation: The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.
authors.forename: John E
authors.initials: JE
authors.lastname: Collins
authors.forename: Melanie E
authors.initials: ME
authors.lastname: Goward
authors.forename: Charlotte G
authors.initials: CG
authors.lastname: Cole
authors.forename: Luc J
authors.initials: LJ
authors.lastname: Smink
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Huckle
authors.forename: Sarah
authors.initials: S
authors.lastname: Knowles
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Bye
authors.forename: David M
authors.initials: DM
authors.lastname: Beare
authors.forename: Ian
authors.initials: I
authors.lastname: Dunham
mesh_terms: D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D005796:Genes / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data