[Cloning and analysis of the regulatory gene fragment induced with seed extract in Rhizobium huakuii strain 7653R].
The large plasmids of strain 7653R were digested with restriction enzyme EcoRI. Their DNA fragments were cloned into the expression vector pMP220 to construct a lacZ fusion pool, which were transferred into the recipient strain 7653R. Tri-transconjugants were selected onto plates containing X-gal and seed extract. Five blue colonies were assayed of their beta-galactosidase activity after incubation with or without seed extract. A positive induced strain HN18 was obtained. Hybridization of nodDABC probe on the recombinant plasmid pHN18 showed a 1.7 kb positive band. The evidence makes a deduction that the pHN18 contains a promoter of nod operon.
Nong
G
G
Laboratories of Agro-Microbiology, Huazhong Agricultural University, Wuhan 430070.
Zhang
Z
Z
Hu
F
F
Chen
H
H
D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D007763:Lac Operon; D010936:Plant Extracts / Q000494:pharmacology*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012231:Rhizobium / Q000235:genetics*; D012639:Seeds / Q000737:chemistry
D004269:DNA, Bacterial; D010936:Plant Extracts