[Characterization of nitric oxide synthase in the chicken cochlea using electrochemical method].
OBJECTIVE
Our investigation was aimed to explore whether certain agonists can induce NO release from neurons of chick cochlea in vitro to characterize NOS isoform in the cochlea.


METHODS
The experiments were performed using CuPtCl6 chemically modified carbon fiber microelectrode under current recording in terms of NO redox activity.


RESULTS
In our previous study it has been shown that solutions containing L-arg, ATP, ACh with Ca2+ can mediate NO release from neurons of chick cochlea, respectively. The present study examined visual current changes induced by other stimuli to cochlear neurons. The solution containing glutamate with Ca2+ could exerted a positive effects and L-arg, ATP, ACh, glutamate without Ca2+ and bradykimin with Ca2+ or without Ca2+ can not induce NO release from the neurons, respectively.


CONCLUSIONS
Based on these results, the NOS isofrom in the chick cochlear neurons is the neuronal isofrom (ncNOS).
Yang
J
J
Department of Otolaryngology, Union Hospital, Tongji Medical University, Wuhan 430022.
Wang
J
J
D000818:Animals; D002645:Chickens; D003051:Cochlea / Q000201:enzymology*; D004563:Electrochemistry / Q000379:methods; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D009474:Neurons / Q000201:enzymology*; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism*
D009569:Nitric Oxide; D019001:Nitric Oxide Synthase