title: [Study on the separation of proteins in human serum by high performance capillary zone electrophoresis].
abstract: A high performance capillary zone electrophoretic method has been developed for the separation of proteins in human serum. Sera were diluted with a buffer (0.05 mol/L boric acid, pH 8.80), and then introduced hydrodynamically into a 37 cm (32 cm to detector) x 50 microns i.d. fused-silica capillary, electrophoresed for 12 min using a boric buffer solution (0.10 mol/L, pH 9.35; containing 4 g/L of polyethylene glycol 8000), and detected at the wavelength of 200 nm. The method is simple, rapid and repeatable.
authors.affiliation: General Hospital of Nanjing Command, PLA, Nanjing 210002, China.
authors.forename: K
authors.initials: K
authors.lastname: Li
authors.forename: Y S
authors.initials: YS
authors.lastname: Yuan
authors.forename: F L
authors.initials: FL
authors.lastname: Zhao
mesh_terms: D000510:Alpha-Globulins / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D012709:Serum Albumin / Q000302:isolation & purification*; D005719:gamma-Globulins / Q000032:analysis
chemical_list: D000510:Alpha-Globulins; D012709:Serum Albumin; D005719:gamma-Globulins