[Effects of growth factors on DNA and collagen synthesis of human mandibular condylar cartilage cells].
OBJECTIVE
To investigate the effects of transforming growth factor-beta 1 (TGF-beta 1), insulin-like growth factor-I (IGF-I) and basic fibroblast growth factor(bFGF) on DNA and collagen synthesis of mandibular condylar cartilage(MCC) of human fetus.


METHODS
Cell culture, 3H-TdR and 3H-Proline incorporation methods were used. MCC cells were harvested from 4 to 5 months old human fetus. Cells were seeded at 2 x 10(4)/well on 96-well Plate. After synchronization, medium was replaced by DMEM containing 0.4% NCS with various growth factors and concentrations.


RESULTS
bFGF stimulated the DNA synthesis significantly, and IGF-I had less effect, while the effect of TGF-beta 1 was insignificant. For collagen synthesis, bFGF caused a dose-dependent increase(60%). A greater effect(98%) was achieved when IGF-1 was added. In contrast, TGF-beta 1 could inhibit collagen synthesis (24%).


CONCLUSIONS
Growth factors play an important part in the proliferation and matrix synthesis of MCC cells, which might be of potential application in treating cartilage destructive lesions.
Jiao
Y
Y
College of Stomatology, West China University of Medical Sciences.
Wang
D
D
Han
W
W
Li
T
T
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism; D024043:Collagen Type II / Q000096:biosynthesis*; D004247:DNA / Q000096:biosynthesis*; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D008335:Mandibular Condyle / Q000166:cytology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D053773:Transforming Growth Factor beta1
D024043:Collagen Type II; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D005346:Fibroblast Growth Factors; D007334:Insulin-Like Growth Factor I; D004247:DNA