[Purification and properties of degrading enzyme of organophosphorus pesticides from Aspergillus ornatus Z58].
Degrading enzyme of organophosophorus pesticides was purified from Aspergillus oryzeZ58 by (NH4)2SO4 precipitation, Sephadex G-100 gel filtration, DEAE-cellulose chromatography with 34.2-fold purification, 17.8% recovery and 76.2 U/mg specific activity. The purified enzyme was homogeneous on polyacrylamide gel electrophoresis. The molecular weight of the enzyme estimated with Sephadex G-100 was 67000. The optimal conditions for activity were pH 7.2, temperature 45 degrees C. The enzyme was stable over the range of pH 6-10 and below 40 degrees C, the activity was inhibited by SDS and stimulated by Cu2+, the substrate ranges were nonspecific to organophosophorus pesticides.
Liu
Y
Y
Department of Biochemistry, School of Life Sciences, Zhongshan University, Guangzhou 510275.
Zhong
Y
Y
D001230:Aspergillus / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D004798:Enzymes / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000378:metabolism*; D008970:Molecular Weight; D009943:Organophosphorus Compounds; D013379:Substrate Specificity; D013696:Temperature
D004798:Enzymes; D007306:Insecticides; D009943:Organophosphorus Compounds