[Effects of mechanical stretch on collagen type I and total protein synthesis of human periodontal ligament fibroblasts].
OBJECTIVE
The aim of this study is to investigate the effects of mechanical stretch on type I collagen and total protein synthesis of human periodontal ligament fibroblasts (PDLFs).


METHODS
Enzyme link immunosorbent assay (ELISA) and Coomassie brilliant blue stain method were used to examine the anabolism of PDLFs growing on the flexible culture plates subjected to mechanical stretch. The five loading devices of PDLFs were designed and the stain varied from 1% to 8%.


RESULTS
Mechanical stretch had no effect on type I collagen synthesis. However, in the range of 1% to 8% of stretch, the protein synthesis was reduced. The mechanical stretching of 2% strain affected the protein synthesis most obviously.


CONCLUSIONS
Mechanical stretch can modify the composition of extracellular matrix of PDLFs.
Chen
X
X
College of Stomatology, West China University of Medical Sciences.
Han
W
W
Zhao
Y
Y
D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology* / Q000378:metabolism; D014176:Protein Biosynthesis; D013314:Stress, Mechanical
D024042:Collagen Type I; D006023:Glycoproteins