title: Application of the AMeX method to the evaluation of HER-2 status in breast carcinomas: comparison with the results of HercepTest.
abstract: The recent development of the recombinant humanized monoclonal antibody against HER-2 oncoprotein requires a simple and accurate method for the evaluation of HER-2 status in patients with breast cancers. We here report that the evaluation of the HER-2 status is improved by the use of the acetone-methanol-xylene (AMeX) method. Compared with an ordinary test of HercepTest, 25 out of 63 cases (39.7%) were scored upwards by the AMeX method. In addition, the HER-2 gene amplification was easily estimated by fluorescence in situ hybridization (FISH) using the AMeX method. Thus, the AMeX method is likely to provide more improved data about the HER-2 status in breast carcinoma specimens.
authors.affiliation: Division of Pathological Diagnosis, Jichi Medical School Hospital, and Department of Pathology, Jichi Medical School, Tochigi, Japan.
authors.forename: Masayo
authors.initials: M
authors.lastname: Mizogami
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakurai
authors.forename: Midori
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Kenichi
authors.initials: K
authors.lastname: Ohta
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Sakurai
authors.forename: Akira
authors.initials: A
authors.lastname: Tanaka
authors.forename: Ken
authors.initials: K
authors.lastname: Saito
mesh_terms: D001943:Breast Neoplasms / Q000737:chemistry / Q000175:diagnosis / Q000235:genetics*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000737:chemistry / Q000175:diagnosis / Q000235:genetics*; D005260:Female; D018734:Genes, erbB-2 / Q000235:genetics*; D016591:Histocytological Preparation Techniques / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D011933:Reagent Kits, Diagnostic; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis / Q000235:genetics*; D015203:Reproducibility of Results
chemical_list: D011933:Reagent Kits, Diagnostic; D018719:Erb-b2 Receptor Tyrosine Kinases