[Analysis of surface-active substances in Sapindus mukurossi by high performance liquid chromatography-mass spectrometry].
A high performance liquid chromatography-atmospheric pressure ionization mass spectrometry method has been developed for the analysis of surface-active substances (hederagenin saponins and sesquiterpene oligoglycosides) in the extracts of the pericarp of Sapindus mukurossi. The method consists of the separation of surface-active substances using C18 HPLC column, followed by detection using a diode-array detector at 210 nm and then on-line mass spectrometry. Hederagenin saponins and sesquiterpene oligoglycosides were characterized as [M - H]- or [M + Na]+. Based on the relative molecular mass, established by mass spectrometry and the structure induced by in-source CID technology, three components that had not been reported in Sapindus mukurossi before were identified. Several surface-active substances were obtained by means of semi-preparative HPLC. Their structures were further confirmed by NMR spectrometry as mukurozi-saponin Y2, mukurozi-saponin X, mukurozioside I a and mukurozioside II a.
Wang
X C
XC
Shanghai White Cat Co. Ltd., Shanghai 200231, China. wcat@public.sta.net.cn
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013058:Mass Spectrometry; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis*; D032127:Sapindus / Q000737:chemistry*; D012717:Sesquiterpenes / Q000032:analysis*
D012717:Sesquiterpenes; D009828:Oleanolic Acid; C025763:hederagenin