title: Generation of hepatocytes expressing functional cytochromes P450 from a pancreatic progenitor cell line in vitro.
abstract: The proliferating AR42J-B13 pancreatic cell line is known to respond to glucocorticoid treatment by producing foci of cells that express the liver-specific albumin gene. We demonstrate that this cell line also expresses liver-specific or liver-enriched functional cytochrome P450 proteins when stimulated to trans-differentiate into hepatocytes by glucocorticoid. These data suggest that this cell line has an unusual ability to trans-differentiate into functional hepatocytes and that it could be possible to generate a limitless supply of functional hepatocyte-like cells in vitro.
authors.affiliation: Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK. m.c.wright@abdn.ac.uk
authors.forename: Carylyn J
authors.initials: CJ
authors.lastname: Marek
authors.forename: Gary A
authors.initials: GA
authors.lastname: Cameron
authors.forename: Lucy J
authors.initials: LJ
authors.lastname: Elrick
authors.forename: Gabrielle M
authors.initials: GM
authors.lastname: Hawksworth
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Wright
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology*; D005938:Glucocorticoids / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D008297:Male; D010179:Pancreas / Q000166:cytology*; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000378:metabolism
chemical_list: D005938:Glucocorticoids; D013739:Testosterone; D003907:Dexamethasone; D003577:Cytochrome P-450 Enzyme System