title: Structure-antibacterial activity relationship of cecropin A derivatives.
abstract: To investigate the effect of net positive charge, alpha-helicity and hydrophobicity of the peptides on antibacterial activity, we designed three kinds of cecropin A (CA) derivatives. Compared to CA, F3 with the highest net positive charge exhibited similar or slightly weaker antibacterial activity (MIC: 3.13 approximately 6.25 microM). F1 showed lower antibacterial activity than that of F3, even though it has higher hydrophobicity and a-helicity than F3. This result indicates that the net positive charge of cecropin A-like peptides appears to be a more important factor in antibacterial activity than alpha-helicity and hydrophobicity. The two peptides F1 and F2 possessed almost similar antibacterial activity, but F2 showed very lower activity in the membrane disruption than F1, suggesting other factors are involved in the antibacterial activity of the peptides as well as peptide-lipid interaction.
authors.affiliation: Research Center for Proteineous Materials, Chosun University, Kwangju 501-759, Korea. syshin@chosun.ac.kr
authors.forename: Song Yub
authors.initials: SY
authors.lastname: Shin
authors.forename: Keun Woo
authors.initials: KW
authors.lastname: Lee
authors.forename: Yangmee
authors.initials: Y
authors.lastname: Kim
authors.forename: Jae Il
authors.initials: JI
authors.lastname: Kim
authors.forename: Kyung-Soo
authors.initials: KS
authors.lastname: Hahm
authors.forename: Shin-Won
authors.initials: SW
authors.lastname: Kang
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000378:metabolism*; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism*; D002942:Circular Dichroism; D009036:Moths / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D010455:Peptides; C031161:cecropin A