title: Range of separation of potential tool for bioseparation, microchip electrophoresis system, for DNA polymorphisms on the human Y-chromosome.
abstract: For the requirement of a high, fast and sufficient technology to suit the needs of 21st century biotechnology, the separation range of a microchip electrophoresis system was studied. Two DNA fragments on the human Y-chromosome, SY594 (82 bp) and 12f2 (88 bp), were successfully separated with a reproducibility of 1.9% and an accuracy of 2.8%. Then, a mixture of 10 DNA markers ranging from 61 bp to 189 bp was successfully separated with high resolution. All of these results demonstrate the superiority of microchip electrophoresis as a tool for 21st century bioseparation.
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, CREST, Japan Science and Technology Corporation, Tokushima 770-8505, Japan. c400141005@stud.tokushima-u.ac.jp
authors.forename: Mohammad
authors.initials: M
authors.lastname: Jabasini
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Fuquan
authors.initials: F
authors.lastname: Dang
authors.forename: Toshikatsu
authors.initials: T
authors.lastname: Shinka
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakahori
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D041322:Chromosomes, Human, Y; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008904:Miniaturization; D011110:Polymorphism, Genetic