[Location of the binary toxin gene of Bacillus sphaeriucs and some biological characteristics of its asprogenous mutants].
Location of the binary toxin gene of Bs C3-41 and some biological characteristics of its asporogenous mutants which obtained by DES treatment were studied in this paper. Mutant C4, L5 with blocked at the stage II of sporulation course were not possessed of any crystalline inclusion, but the binary toxin proteins were detected in their cells by SDS-PAGE analysis. Another mutant G5 which blocked at the stage III of sporulation course formed parasporal crystal in the cells, and its toxicity to larvae of Culex quinquefasciatus was about 50 times higher than those of mutant C4 and L5. The result of curing plasmid and Southern blot analysis confirm the binary toxin gene of Bs C3-41, Bs 1691 and Bs-10 locate on the chromosome.
Liu
Y
Y
Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071.
Sun
F
F
Yuan
Z
Z
Liu
E
E
Zhang
Y
Y
D000818:Animals; D001407:Bacillus / Q000235:genetics* / Q000502:physiology; D001427:Bacterial Toxins / Q000032:analysis / Q000235:genetics*; D003465:Culex / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D009154:Mutation; D013171:Spores, Bacterial / Q000235:genetics
D001427:Bacterial Toxins; C046134:binB protein, Bacillus sphaericus