Construction of designer glycoconjugate vaccines with size-specific oligosaccharide antigens and site-controlled coupling.
Coupling of carbohydrate antigens to protein carriers is a typical approach to enhancing the immunogenicity of carbohydrate-based vaccines. Glycoconjugates with well-defined structures are needed for studies defining the structural variables that govern antibody responses. We report a chemical strategy for preparation of an array of glycoconjugates containing saccharides of desired molecular sizes by selective depolymerization of bacterial polysaccharides and chemically controlled site-specific coupling. As an example, we synthesized and evaluated an oligosaccharide-based vaccine against type III group B Streptococcus.
Wang
Julia Y
JY
Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA. ywang@channing.harvard.edu
Chang
Alex H C
AH
Guttormsen
Hilde-Kari
HK
Rosas
Angel L
AL
Kasper
Dennis L
DL
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D016667:Bacterial Capsules; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003503:Cyclobutanes; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006001:Glycoconjugates / Q000138:chemical synthesis / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000276:immunology* / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D022541:Streptococcal Vaccines / Q000276:immunology*; D013292:Streptococcus agalactiae / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D003503:Cyclobutanes; D006001:Glycoconjugates; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D022541:Streptococcal Vaccines; C105866:group B streptococcal type III capsular polysaccharide-tetanus toxoid vaccine; C032263:streptococcal polysaccharide type III group B; C030042:squaric acid