title: Structural characterization of the O-chain polysaccharide isolated from Bordetella avium ATCC 5086: variation on a theme(1).
abstract: The O-chain polysaccharide (O-PS) of Bordetella avium was isolated from the lipopolysaccharide by mild acid hydrolysis to remove the lipid A, followed by hydrofluorolysis to remove the lipopolysaccharide core oligosaccharide leaving a residual O-PS for structural analysis. High resolution (1)H and (13)C NMR and MALDI studies showed the O-chain to be a polymer composed of 1,4-linked 2-acetamidino-3-[3-hydroxybutanamido]-2,3-dideoxy-beta-D-glucopyranosyluronic acid residues.
authors.affiliation: Institute for Biological Sciences, NRC, Ottawa, ON, Canada.
authors.forename: Suzon
authors.initials: S
authors.lastname: Larocque
authors.forename: Jean-Robert
authors.initials: JR
authors.lastname: Brisson
authors.forename: Hélène
authors.initials: H
authors.lastname: Thérisod
authors.forename: Malcolm B
authors.initials: MB
authors.lastname: Perry
authors.forename: Martine
authors.initials: M
authors.lastname: Caroff
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000737:chemistry; D000081:Acetamides / Q000737:chemistry; D001884:Bordetella / Q000737:chemistry*; D002247:Carbon Isotopes; D004591:Electrophoresis, Polyacrylamide Gel; D006858:Hydrofluoric Acid / Q000737:chemistry; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000081:Acetamides; D002247:Carbon Isotopes; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; C030686:acetamide; D006858:Hydrofluoric Acid; D020155:3-Hydroxybutyric Acid