[Establishment of a GC and GC/MS method for analysis of fatty acids in human dental plaque].
OBJECTIVE
To study the composition of fatty acids in human dental plaque, a Gas Chromatography (GC) and Gas Chromatography/Mass Spectrometry (GC/MS) method was developed.


METHODS
The C17:0 was internal standard and the C16:0 was test standard. Samples of plaque were methanolysis to be fatty acids methyl ester before detection. GC with OV-1 separated the sample for a quantitative analysis. The column size was 25 m x 25 mm. The temperature program was from 80 degrees C to 260 degrees C with 12 degrees C/min. The sample separation was carried out at fused silica capillary column with OV-1 for qualitative analysis by GC/MS. The column size was 23 m x 0.23 mm. The temperature program was from 40 degrees C to 240 degrees C at 6 degrees C/min.


RESULTS
The linear regression equation was y = 0.0138X-0.1182, r = 0.9945, recovery was 90.18%-103.6%, CV intro-day was 2.60, 1.61, 2.20; CV inter-day was; 2.71, 1.69, 1.61. The lowest limit of detection was 0.6 ug. The GC and GC/MS diagram showed that internal standard and fatty acids of plaque have good separation.


CONCLUSIONS
The method is simple, rapid accurate, and is useful to carry out qualitative and quantitative analyses of fatty acids in dental plaque.
Li
J
J
College of Stomatology, West China University of Medical Sciences.
Zhou
X
X
Dai
C
C
Zhang
P
P
D002849:Chromatography, Gas / Q000379:methods; D003773:Dental Plaque / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans
D005227:Fatty Acids