title: [An evaluation of adenosine triphosphate bioluminescence assay for tumor in vitro chemosensitivity testing].
abstract: This study was aimed at the feasibility of using ATP-bioluminescence assay for tumor in vitro chemosensitivity testing. With the use of this assay, the authors determined dose-response curve in mouse fibroma cell line L929 treated with chemotherapeutic agents, and investigated the different in vitro responses of 6 ovarian carcinomas (5 from fresh tumor tissues, 1 from ascites) treated with etopside, cis-plating, 5-fluorouracil and adriamycin. The results showed that the coefficients of variation for triplicate assays ranged from 1.2% to 15.8% which means high reproducibility of the assay. The single cell suspension (including < 30 cells clusters) could be separated from tissue fragments by means of enzyme cocktail (collagenase, Dnase, pronase). The viable cells were over 90%. This study demonstrates that ATP-bioluminescence assay is a sensitive, reliable and efficient method for tumor chemosensitivity testing. In this connection, the correlation between in vitro drug sensitivity and in vivo patient response is worth further studying.
authors.affiliation: Department of Obstetrics and Gynecology, Second Affiliated Hospital, WCUMS, Chengdu 610041.
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.forename: Z
authors.initials: Z
authors.lastname: Peng
authors.forename: J
authors.initials: J
authors.lastname: Lou
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: Q
authors.initials: Q
authors.lastname: Tang
authors.forename: B
authors.initials: B
authors.lastname: He
mesh_terms: D000255:Adenosine Triphosphate; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002945:Cisplatin / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D005260:Female; D005350:Fibroma / Q000473:pathology*; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D008163:Luminescent Measurements; D051379:Mice; D010051:Ovarian Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D004317:Doxorubicin; D000255:Adenosine Triphosphate; D002945:Cisplatin; D005472:Fluorouracil