[Determination of nanchangmycin and meilingmycin by high performance liquid chromatography].
A high performance liquid chromatographic method had been established for the separation and determination of two antibiotics produced by Streptomyces nanchangensis, polyether nanchangmycin and 16-membered macrolide meilingmycin. The latter is composed of several components. The operating conditions were Waters XTerra RP18 column (3.9 mm i.d. x 150 mm, 5 microns) at 25 degrees C, mobile phase linear gradient elution of acetonitrile-water with 57:43 (volume ratio) during 0 min-30 min, 70:30 during 30 min-32 min, 80:20 during 32 min-34 min, 90:10 during 34 min-50 min at a flow rate of 0.8 mL/min, and photodiode array detector at 234 nm. This method is accurate, rapid and simple, and can be used for the integrated analysis of the two different natural compounds, polyethers and macrolides.
Sun
Yu-hui
YH
Bio-X Life Science Research Center, College of Life Science & Technology, Shanghai Jiaotong University, Shanghai 200030, China.
Zhou
Xiu-fen
XF
Tu
Guo-quan
GQ
Deng
Zi-xin
ZX
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004987:Ethers / Q000032:analysis* / Q000302:isolation & purification; D018942:Macrolides; D013141:Spiro Compounds / Q000032:analysis* / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D004987:Ethers; D018942:Macrolides; D013141:Spiro Compounds; C452420:meilingmycin; C452419:nanchangmycin