[Study on optimization of the required amount of mobile phase for preparative unit of simultaneous renaturation and purification of proteins].
Based on the stoichiometric displacement model for retention (SDM-R) in high performance liquid chromatography(HPLC), the biopolymer retentions are mainly dominated by displacer's concentration in mobile phase. According to this conclusion, an equation is presented for evaluating the minimum amount of mobile phase used for the preparative purpose with the unit of simultaneous renaturation and purification of proteins (USRPP) by linear gradient elution. Furthermore, the resolution and refolding efficiency of lysozyme were found to be independent of the flow-rate and gradient time when the amount of mobile phase was kept constant. The conclusions were tested by experiments and a good coincidence was obtained.
Zhang
Y J
YJ
Shaaxi Provincial Key Laboratory of Modern Separation Science, Institute of Modern Separation Science, Northwest University, Xi'an 710069, China.
Li
X
X
Geng
X D
XD
D001704:Biopolymers; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D003574:Cytochrome c Group / Q000032:analysis / Q000302:isolation & purification; D046911:Macromolecular Substances; D009113:Muramidase / Q000032:analysis / Q000302:isolation & purification*; D009211:Myoglobin / Q000032:analysis / Q000302:isolation & purification; D020673:Protein Renaturation
D001704:Biopolymers; D003574:Cytochrome c Group; D046911:Macromolecular Substances; D009211:Myoglobin; D009113:Muramidase