A simple and sensitive HPLC method for determination of gliclazide in human serum.
A simple, rapid and specific method for analysis of gliclazide in serum by a sensitive high-performance liquid chromatographic method is described. Only 100 microl of serum and a little sample work-up is required. A simple procedure of extraction by toluene followed by evaporation to dryness under a gentle stream of air and dissolving the dried residue in mobile was used. The gliclazide peak was separated from endogenous peaks on a C(8) column by a mobile phase of acetonitrile-water (45:55, v/v), pH 3. Gliclazide and internal standard (phenytoin) were eluted at 6.8 and 3.8 min, respectively. The limit of quantitation (LOQ) for gliclazide in serum was 75 ng/ml at 230 nm. The method was linear over the range of 75-10,000 ng/ml with r(2) of 0.999. Mean recovery for gliclazide and internal standard was 84.5 and 87%, respectively.
Rouini
Mohammad-Reza
MR
Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14155-6451, Iran. rouini@sina.tums.ac.ir
Mohajer
Afshin
A
Tahami
Mohammad-Hosein
MH
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D018592:Cross-Over Studies; D004311:Double-Blind Method; D005907:Gliclazide / Q000097:blood* / Q000493:pharmacokinetics; D006207:Half-Life; D006801:Humans; D007004:Hypoglycemic Agents / Q000097:blood* / Q000493:pharmacokinetics; D007202:Indicators and Reagents; D008297:Male; D010672:Phenytoin / Q000097:blood; D012015:Reference Standards; D015203:Reproducibility of Results
D007004:Hypoglycemic Agents; D007202:Indicators and Reagents; D010672:Phenytoin; D005907:Gliclazide