[Determination of tetramethylpyrazine in animal serum and cerebrospinal fluid by high performance liquid chromatography].
A reversed-phase high performance liquid chromatographic (RP-HPLC) method for the determination of the tetramethylpyrazine(TMP) in Chuanxiong extract, the animal(mouse) serum and cerebrospinal fluid has been developed. The TMP was separated on an ODS column Zorbax SB-C18(4.6 mm i.d. x 250 mm, 5 microns) at room temperature and detected by using UV detector at 270 nm. The mobile phase was methanol-water (50:50, V/V) containing 0.2 mmol/L of NH4H2PO4 flowing at a rate of 0.8 mL/min and 20 microL samples were injected. The detection limit of TMP was 1 mg/L and the calibration curve is linear between 5 and 500 mg/L with a correlation coefficient (r) of 0.999. The recovery of TMP ranged 98%-103%. The extract of Chuanxiong and pretreated serum and cerebrospinal fluid sample are stable for a week at room temperature.
Ding
M Y
MY
Department of Chemistry, Tsinghua University, Beijing 100084, China. dingmy@chem.tsinghua.edu.cn
Luo
S Z
SZ
Liu
H
H
Chen
P R
PR
Liu
D L
DL
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005260:Female; D030004:Ligusticum; D008297:Male; D008516:Medicine, Chinese Traditional; D000432:Methanol; D011719:Pyrazines / Q000097:blood* / Q000134:cerebrospinal fluid*; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D011719:Pyrazines; C101574:chuangxiong extract; C017953:tetramethylpyrazine; D000432:Methanol