[Preparative separation of alkaloids from Cortex Phellodendri by high speed countercurrent chromatography].
High speed countercurrent chromatography was successfully applied to the separation of alkaloids from Cortex Phellodendri with a two-phase solvent system composed of CHCl3-CH3OH-0.5 mol/L HCl (2:1:1, volume ratio). Some alkaloid components were obtained, and identification of berberine was made by thin-layer chromatography.
Yuan
Li-Ming
LM
College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650092, China. yuanlm@public.km.yn.cn
Wu
Ping
P
Xia
Tao
T
Chen
Xue-Xian
XX
D000470:Alkaloids / Q000302:isolation & purification*; D001599:Berberine / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods; D032102:Phellodendron / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry
D000470:Alkaloids; D001599:Berberine