[Construction of full-length cDNA clone and complete nucleotide sequence of RNA2 of cucumber mosaic virus SD strain].
The full-length cDNA clone of CMV-SD RNA2 was constructed from two cDNA clones, which were obtained by RT-PCR with primers synthesized based on terminal sequences determined using 5' RACE and 3' RACE, respectively; and the complete sequence was determined. It consists of 3048 nt and there are two partial overlapped ORFs. ORF1, which is located in the 5' portion (79-2652 nt), codes for 2a protein of 858aa, and GDD consensus sequence reported to be "polymerase site" was found in deduced 2a protein sequence. ORF2, which is located in the 3' portion (2414-2746 nt), codes for 2b protein of 111aa. The overall nucleotide sequence homologies with RNA2 of CMV Fny and Q strain, which are representatives of subgroups I and II, respectively, are 91.7% and 75.6%; the amino acid sequence homologies are 93.8% and 67.7% for 2a protein, and 83.0% and 51.3% for 2b protein, respectively. These results demonstrated that CMV-SD belongs to subgroup I.
Zhang
G
G
Laboratory of Plant Biotechnology, Institute of Microbiology, Academia Sinica, Beijing 100080.
Xu
Y
Y
Cai
W
W
Fang
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017799:Cucumovirus / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012367:RNA, Viral / Q000235:genetics*; D017385:Sequence Homology
D018076:DNA, Complementary; D012367:RNA, Viral