[An improvement on determination of the related substances in vindesine sulfate and its injection].
A new method for the analysis of related substances in vindesine sulfate and its injection to improve the ability of separation by RP-HPLC was established. The separation was achieved by applying a reversed-phase C18 column (150 mm x 4.6 mm i.d.) and a mixture of V(MeOH):V(buffer solution) = 70:30 as mobile phase. The buffer solution was an aqueous solution of volume fraction 1.5% diethylamine adjusted to pH 7.5 with phosphoric acid. The UV-detector was set at 267 nm. Four related substances were separated from vindesine within thirteen minutes. The tailing factor of the peak of vindesine is 1.00-1.05. The results showed that this method is more efficient for separating the related substances in vindesine sulfate than the conventional method.
Chen
J
J
Zhejiang Institute for Drug Control, Hangzhou, 310004.
Huang
Z
Z
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004340:Drug Contamination / Q000517:prevention & control*; D007267:Injections; D014751:Vindesine / Q000032:analysis* / Q000302:isolation & purification*
D014751:Vindesine