[Production of vitamin C precursor--2-keto-L-gulonic acid from D-sorbitol by mixed culture of microorganisms].
Gluconobacter oxydans SCB329 only produce a little amount of 2-Keto-L-gulonic acid(2-KLG) from D-Sorbitol when growing alone; while Gluconobacter sp. SCB110 can transform D-Sorbitol to L-Sorbose and can not produce 2-KLG. 2-Keto-L-gulonic acid, the precursor of L-Ascorbic acid (Vitamin C) synthesis, was prepared directly with a high efficiency from D-Sorbitol by mixed culture of microorganism, which comprised Gluconobacter sp. SCB110 and Gluconobacter oxydans SCB329. The fermentation product from the mixed culture broth in the D-Sorbotol-containing medium was identified as 2-Keto-L-gulonic acid by HPLC, elementary analysis and infra-red adsorption spectrum.
Yin
G
G
Shanghai Research Center of Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China.
Lin
W
W
Qiao
C
C
Ye
Q
Q
D018920:Coculture Techniques; D005285:Fermentation; D020576:Gluconobacter / Q000378:metabolism*; D020600:Gluconobacter oxydans / Q000378:metabolism*; D013012:Sorbitol / Q000378:metabolism*; D013400:Sugar Acids / Q000378:metabolism*
D013400:Sugar Acids; D013012:Sorbitol; C011491:provitamin C