[The important role of vitamins in the over-production of pyruvic acid].
The effect of nicotinic acid, thiamine, pyridoxine, biotin and riboflavin on the production of pyruvic acid by Torulopsis glabrata WSH-IP303 with glucose as carbon source and NH4Cl as sole nitrogen source was investigated. By using orthogonal experiment method, thiamine was confirmed to be the most important factor affecting the production of pyruvic acid. Based on a certain concentration range of thiamine (0.01-0.015 mg/L), glucose consumption rate can be enhanced by increasing the concentration of nicotinic acid. When the concentration of nicotinic acid, thiamine, pyridoxine, biotin and riboflavin were 8, 0.015, 0.4, 0.04 and 0.1 mg/L, respectively, the concentration and yield to glucose of pyruvic acid reached 52.4 g/L and 0.525 g/g at 48 h in flask culture, respectively. Batch culture was conducted in a 2.5 L fermentor with initial glucose concentration of 120 g/L. By adopting the optimal concentration combination of vitamins, the concentration and yield to glucose of pyruvic acid reached 69.4 g/L and 0.593 g/g at 57.5 h, which were increased by 32.4% and 13% than the best results in flask culture, respectively.
Li
Y
Y
Lab of Environmental Biotechnology, School of Biotechnology, Wuxi University of Light Industry, Wuxi 214036.
Chen
J
J
Lun
S
S
Rui
X
X
D000643:Ammonium Chloride / Q000378:metabolism; D001710:Biotin / Q000494:pharmacology; D041221:Candida glabrata / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D009525:Niacin / Q000494:pharmacology; D011736:Pyridoxine / Q000494:pharmacology; D019289:Pyruvic Acid / Q000378:metabolism*; D012256:Riboflavin / Q000494:pharmacology; D013831:Thiamine / Q000494:pharmacology*; D014815:Vitamins / Q000494:pharmacology*
D014815:Vitamins; D000643:Ammonium Chloride; D009525:Niacin; D001710:Biotin; D019289:Pyruvic Acid; D005947:Glucose; D011736:Pyridoxine; D012256:Riboflavin; D013831:Thiamine