Interplay of tumor vascular oxygenation and tumor pO2 observed using near-infrared spectroscopy, an oxygen needle electrode, and 19F MR pO2 mapping.
This study investigates the correlation of tumor blood oxygenation and tumor pO(2) with respect to carbogen inhalation. After having refined and validated the algorithms for calculating hemoglobin concentrations, we used near-infrared spectroscopy (NIRS) to measure changes of oxygenated hemoglobin concentration (delta[HbO(2)]) and used an oxygen needle electrode and (19)F MRI for pO(2) measurements in tumors. The measurements were taken from Dunning prostate R3327 tumors implanted in rats, while the anesthetized rats breathed air or carbogen. The NIRS results from tumor measurements showed significant changes in tumor vascular oxygenation in response to carbogen inhalation, while the pO(2) electrode results showed an apparent heterogeneity for tumor pO(2) response to carbogen inhalation, which was also confirmed by (19)F MR pO(2) mapping. Furthermore, we developed algorithms to estimate hemoglobin oxygen saturation, sO(2), during gas intervention based on the measured values of delta[HbO(2)] and pO(2). The algorithms have been validated through a tissue-simulating phantom and used to estimate the values of sO(2) in the animal tumor measurement based on the NIRS and global mean pO(2) values. This study demonstrates that the NIRS technology can provide an efficient, real-time, noninvasive approach to monitoring tumor physiology and is complementary to other techniques, while it also demonstrates the need for an NIR imaging technique to study spatial heterogeneity of tumor vasculature under therapeutic interventions.
Kim
Jae G
JG
University of Texas at Arlington/University of Texas Southwestern Medical Center at Dallas, Joint Graduate Program in Biomedical Engineering, Arlington, Texas 76019, USA.
Zhao
Dawen
D
Song
Yulin
Y
Constantinescu
Anca
A
Mason
Ralph P
RP
Liu
Hanli
H
D000465:Algorithms; D000818:Animals; D002245:Carbon Dioxide; D004566:Electrodes; D005461:Fluorine; D008279:Magnetic Resonance Imaging; D008297:Male; D010100:Oxygen / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D010108:Oxyhemoglobins / Q000378:metabolism; D019047:Phantoms, Imaging; D011471:Prostatic Neoplasms / Q000097:blood / Q000098:blood supply* / Q000378:metabolism*; D051381:Rats; D019265:Spectroscopy, Near-Infrared / Q000295:instrumentation / Q000379:methods*
D010108:Oxyhemoglobins; D002245:Carbon Dioxide; D005461:Fluorine; C011700:carbogen; D010100:Oxygen