Clonal distribution of an atypical MRP+, EF*, and suilysin+ phenotype of virulent Streptococcus suis serotype 2 strains in Brazil.
Streptococcus suis is considered one of the most important bacterial swine pathogens worldwide. The distribution of the 35 described serotypes in diseased animals may vary in different regions. Data regarding S. suis isolation from pigs in South America is not available. In the present study, 51 isolates of S. suis recovered in pure culture or as the predominant species from diseased animals in Brazil, were analyzed. These isolates were classified as serotypes 2 (58.8%), 3 (21.5%), 7 (13.7%), 1 (3.9%), and 14 (2%). Serotype 2 isolates were further studied for their production of virulence-related proteins muramidase-released protein (MRP), extracellular factor (EF), and suilysin. In addition, the genetic diversity was studied by randomly amplified polymorphic DNA. All but 1 of the serotype 2 isolates showed a clonal distribution of an atypical phenotype (MRP+, EF*, suilysin+), different from the known European (MRP+, EF+, suilysin+), and North American (MRPv, EF-, suilysin-), phenotypes.
Martinez
G
G
Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de médecine vétérinaire, Université de Montreal, CP 5000, St-Hyacinthe, Québec J2S 7C6.
Pestana de Castro
A F
AF
Ribeiro Pagnani
K Jordão
KJ
Nakazato
G
G
Dias da Silveira
W
W
Gottschalk
M
M
D000818:Animals; D000942:Antigens, Bacterial; D016667:Bacterial Capsules; D001426:Bacterial Proteins / Q000378:metabolism*; D001938:Brazil; D006460:Hemolysin Proteins / Q000378:metabolism*; D009930:Organic Chemicals; D010641:Phenotype; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D012703:Serotyping / Q000662:veterinary; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D017011:Streptococcus suis / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D013553:Swine Diseases / Q000382:microbiology*; D014774:Virulence
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C082316:Epf protein, Streptococcus suis; D006460:Hemolysin Proteins; C421489:MRP protein, Streptococcus suis; D009930:Organic Chemicals; C104760:suilysin