title: [Separation of saccharides in tobacco by capillary zone electrophoresis].
abstract: A method for the separation of derivatized saccharides in tobacco with high performance capillary electrophoresis and ultraviolet spectrophotometry (HPCE-UV) is presented. Five saccharides in tobacco were derivatized with p-aminobenzonitrile, separated at pH 10.5 with 50 mmol/L borate buffer containing 5% (volume fraction, the same in the following percentage concentration) methanol, 5% acetonitrile, 2.5% ethyleneglycol, 2.5% 2-propanol and 1 mmol/L sodium dodecyl sulfate, and detected at 285 nm.
authors.affiliation: Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ma
authors.forename: You-zhao
authors.initials: YZ
authors.lastname: He
authors.forename: Xie-zhong
authors.initials: XZ
authors.lastname: Xiao
authors.forename: Rui
authors.initials: R
authors.lastname: Huang
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Xu
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D005947:Glucose / Q000302:isolation & purification; D008320:Maltose / Q000302:isolation & purification; D009005:Monosaccharides / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D014026:Nicotiana / Q000737:chemistry*; D014994:Xylose / Q000302:isolation & purification
chemical_list: D009005:Monosaccharides; D008320:Maltose; D014994:Xylose; D005947:Glucose