title: Iron uptake by hepatopancreas brush border membrane vesicles (BBMV) of the lobster (Homarus americanus).
abstract: The uptake of (55)Fe(2+) and solubilized (55)Fe(3+) into brush border membrane vesicles prepared from the hepatopancreas of the Atlantic lobster (Homarus americanus) was investigated. Non-specific surface binding of (55)Fe(2+) at equilibrium to the vesicular surface approximated 57% of total (55)Fe(2+) uptake. (55)Fe(2+) uptake showed temperature sensitivity and was trans-stimulated by a Ca(2+) gradient (at 5mM) directed out. Equilibrated (59)Fe(2+) exchanged for both Cd(2+) and cold Fe(2+). The data obtained in this study are suggestive that at least a portion of ferrous iron absorption may occur by a divalent exchanger mechanism.
authors.affiliation: Department of Physiology, University of Hawaii, Honolulu, Hawaii 96822, USA.
authors.forename: Amy G
authors.initials: AG
authors.lastname: Aslamkhan
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Ahearn
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002104:Cadmium / Q000378:metabolism; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002413:Cations, Divalent / Q000378:metabolism / Q000494:pharmacology; D004064:Digestive System / Q000166:cytology* / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism*; D008121:Nephropidae / Q000166:cytology / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D015032:Zinc / Q000494:pharmacology
chemical_list: D002413:Cations, Divalent; D002104:Cadmium; D007501:Iron; D015032:Zinc; D002118:Calcium