title: Modulation of J774.1 macrophage L-arginine metabolism by intracellular Mycobacterium bovis BCG.
abstract: Using a Mycobacterium bovis BCG mutant (AS1) lacking a Bacillus subtilis L-arginine transporter homolog, we demonstrate here that the interaction between intracellular mycobacteria and the macrophage with respect to L-arginine transport and metabolism is quite complex. Intracellular AS1 stimulates macrophage L-arginine transport and accumulates 2.5-fold more (3)H label derived from L-arginine than does the wild type. These studies suggest that the accumulation of (3)H label reflects the acquisition of metabolites of L-arginine produced by the macrophage.
authors.affiliation: Department of Microbiology and Molecular Genetics, International Center for Public Health, UMDNJ/New Jersey Medical School, Newark 07101-1709, USA.
authors.forename: Marcy A
authors.initials: MA
authors.lastname: Peteroy-Kelly
authors.forename: Vishwanath
authors.initials: V
authors.lastname: Venketaraman
authors.forename: Meliza
authors.initials: M
authors.lastname: Talaue
authors.forename: Anjali
authors.initials: A
authors.lastname: Seth
authors.forename: Nancy D
authors.initials: ND
authors.lastname: Connell
mesh_terms: D026905:Amino Acid Transport Systems / Q000235:genetics; D026923:Amino Acid Transport Systems, Basic; D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001692:Biological Transport; D002460:Cell Line; D003470:Culture Media; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology*; D051379:Mice; D009154:Mutation; D009163:Mycobacterium bovis / Q000235:genetics / Q000378:metabolism*; D014316:Tritium / Q000378:metabolism
chemical_list: D026905:Amino Acid Transport Systems; D026923:Amino Acid Transport Systems, Basic; D003470:Culture Media; D014316:Tritium; C043909:arginine permease; D001120:Arginine