[Preparation and evaluation of glycine derivatized beta-cyclodextrin bonded silica for high performance liquid chromatography].
Glycine derivatized beta-cyclodextrin bonded silica (GCDS) has been prepared for high performance liquid chromatography through the reactions of beta-cyclodextrin bonded silica with tosyl chloride and glycine in sequence. The separation performance of GCDS for positional isomers, dansyl amino acids and phenylpropionic acids was investigated. It was found that nitrophenols could not be eluted with methanol as mobile phase, but could be eluted with methanol-phosphate buffer solution within appropriate time, which was much different from the behavior observed on native beta-cyclodextrin bonded silica. The effect of column temperature on the enantio-separation of dansyl D,L-Phe with GCDS was examined. The results show that the enantio-separation ability of the GCDS decreases with the increase of the column temperature. Other factors such as methanol concentration and pH of the mobile phase influencing the retention behavior of solutes on the GCDS are also discussed.
Feng
Y Q
YQ
College of Chemistry and Environmental Science, Wuhan University, Wuhan 430072, China. yqfeng@public.wh.hb.cn
Da
S L
SL
D000596:Amino Acids / Q000302:isolation & purification; D000814:Aniline Compounds / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D003505:Cyclodextrins; D005998:Glycine; D009596:Nitrophenols / Q000302:isolation & purification; D010666:Phenylpropionates / Q000302:isolation & purification; D058428:Silica Gel; D012822:Silicon Dioxide; D047392:beta-Cyclodextrins
D000596:Amino Acids; D000814:Aniline Compounds; D003505:Cyclodextrins; D009596:Nitrophenols; D010666:Phenylpropionates; D047392:beta-Cyclodextrins; C008625:nitroaniline; D058428:Silica Gel; C019521:phenylpropiolic acid; D012822:Silicon Dioxide; C031215:betadex; D005998:Glycine