Mild thyroid hormones deficiency modifies benzodiazepine and mu-opioid receptor binding in rats.
The effects of a mild hypothyroidism condition on benzodiazepine (BDZ) and mu opioid receptor levels was investigated. Female Wistar rats were randomly divided into two groups: 1) hypothyroid rats (n=7), which received methimazole (60 mg/kg per day) in drinking water for four weeks, and 2) euthyroid rats (n=8), which drank only tap water. Animals were sacrificed and their brains were used for autoradiography experiments. When compared to the euthyroid group, the hypothyroid group presented reduced benzodiazepine receptor binding in medial amygdala (24%) and high mu-receptor levels in frontal (25%), sensorimotor (65%) and temporal (29%) cortices, basolateral amygdala (50%) and ventroposterior thalamic nucleus (49%). The present data suggest that alterations in BDZ and mu-receptor binding could be associated with the higher excitability observed in animals with triiodothyronine (T(3)) deficiency.
Ortiz-Butron
R
R
Departamento de Fisiología Mauricio Russek, Escuela Nacional de Ciencias Biológicas, I.P.N., Carpio y Plan de Ayala, Mexico, D.F., C.P. 11340. rocipn@yahoo.com.mx
Pacheco-Rosado
J
J
Hernández-Garcia
A
A
Briones-Velasco
M
M
Rocha
L
L
D000818:Animals; D013956:Antithyroid Agents; D001345:Autoradiography; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / Q000378:metabolism; D005260:Female; D005445:Flunitrazepam / Q000378:metabolism; D018755:GABA Agonists / Q000378:metabolism; D058785:GABA-A Receptor Agonists; D007037:Hypothyroidism / Q000139:chemically induced / Q000378:metabolism; D008713:Methimazole; D011869:Radioligand Assay; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000378:metabolism*; D017450:Receptors, Opioid, mu / Q000819:agonists / Q000378:metabolism*; D013974:Thyroxine / Q000097:blood*; D014284:Triiodothyronine / Q000097:blood / Q000172:deficiency*
D013956:Antithyroid Agents; D018755:GABA Agonists; D058785:GABA-A Receptor Agonists; D011963:Receptors, GABA-A; D017450:Receptors, Opioid, mu; D014284:Triiodothyronine; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D008713:Methimazole; D005445:Flunitrazepam; D013974:Thyroxine