Modeled channel distributions explain extracellular recordings from cultured neurons sealed to microelectrodes.
Amplitudes and shapes of extracellular recordings from single neurons cultured on a substrate embedded microelectrode depend not only on the volume conducting properties of the neuron-electrode interface, but might also depend on the distribution of voltage-sensitive channels over the neuronal membrane. In this paper, finite-element modeling is used to quantify the effect of these channel distributions on the neuron-electrode contact. Slight accumulation or depletion of voltage-sensitive channels in the sealing membrane of the neuron results in various shapes and amplitudes of simulated extracellular recordings. However, estimation of channel-specific accumulation factors from extracellular recordings can be obstructed by co-occuring ion currents and defect sealing. Experimental data from cultured neuron-electrode interfaces suggest depletion of sodium channels and accumulation of potassium channels.
Buitenweg
Jan Reinoud
JR
Institute for Biomedical Technology, Signals and Systems Group, Faculty of Electrical Engineering, University of Twente. PO Box 217, 7500 AE Enschede, The Netherlands. J.R.Buitenweg@el.utwente.nl
Rutten
Wim L C
WL
Marani
Enrico
E
D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D002448:Cell Adhesion; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D003198:Computer Simulation; D004574:Electromagnetic Fields; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D005110:Extracellular Space / Q000502:physiology*; D020342:Finite Element Analysis; D005727:Ganglia, Spinal / Q000502:physiology; D015640:Ion Channel Gating / Q000502:physiology*; D007473:Ion Channels / Q000502:physiology; D008839:Microelectrodes; D008959:Models, Neurological; D009474:Neurons / Q000502:physiology*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015222:Sodium Channels / Q000502:physiology*
D020099:Coated Materials, Biocompatible; D007473:Ion Channels; D015222:Sodium Channels