[Effects of acoustic stimuli on intracellular free calcium isolated cochlear OHC].
OBJECTIVE
To study the effects of acoustic stimuli upon intracellular free calcium concentration ([Ca2+]i) of cochlear outer hair cells (OHCs) isolated from guinea pigs.


METHODS
The specimens were stained with fluorescence, and measured by using ACAS 570.


RESULTS
Acoustic stimuli caused a remarkable increase of [Ca2+]i and the concentration of [Ca2+]i decreased gradually after reaching its peak value. The reponeses can repeat with another stimuli.


CONCLUSIONS
The acoustic stimuli could alter the physiologic characteristics of single OHC.
Li
Y
Y
Zhujiang Hospital, First Military Medical University, Guangzhou 510282.
Huang
Y
Y
Wang
P
P
D000161:Acoustic Stimulation; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male
D002118:Calcium