Anti-allergic action of buckwheat (Fagopyrum esculentum Moench) grain extract.
The anti-allergic action of buckwheat grain extract (BGE) was investigated using rodent experimental models. The oral, intraperitoneal and intradermal administration of BGE significantly inhibited the compound 48/80-induced vascular permeability documented by Evans blue extravasation. In addition, BGE showed potent inhibitory effect on passive cutaneous anaphylaxis (PCA) activated by anti-dinitrophenyl (DNP) IgE when orally administered. In an in vitro study, BGE revealed to possess inhibitory potential on the compound 48/80-induced histamine release from rat peritoneal mast cells (RPMC). Moreover, BGE inhibited the IL-4 and TNF-alpha mRNA induction by PMA and A23187 in human leukemia mast cells, HMC-1. Taken together, these results suggest that anti-allergic action of BGE may be due to the inhibition of histamine release and cytokine gene expression in the mast cells.
Kim
Chang Deok
CD
Oriental Hospital, Oriental Medical College of Daejeon University, 22-5 Daeheung-dong, Daejeon 301-724, South Korea.
Lee
Won-Kyung
WK
No
Kyong-Ok
KO
Park
Sang-Ki
SK
Lee
Min-Ho
MH
Lim
Seok Rhin
SR
Roh
Seok-Seon
SS
D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D019612:Fagopyrum / Q000737:chemistry*; D006632:Histamine / Q000378:metabolism; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D018926:Anti-Allergic Agents; D016207:Cytokines; D010936:Plant Extracts; D012333:RNA, Messenger; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine