Recruitment of postmitotic neurons into the regenerating spinal cord of urodeles.
By using fluorescent tracers, we have investigated the origin of the cells that form the regenerating spinal cord after tail amputation in urodele amphibians. We show that spinal cord cells immediately adjacent to the amputation plane die and are removed by phagocytic cells. Spinal cells just anterior to these dying cells are destined to make the majority of the regenerating cord. The largest contribution is likely to come from the radial ependymal cells, but we also demonstrate that postmitotic neurons in this location can translocate into the regenerating cord. These neurons integrate into the regenerate structure and survive for at least 4 weeks. We find no evidence that these translocated neurons dedifferentiate and divide during this regeneration process. We discuss the possibility that these neurons survive long term in the regenerate cord and become part of the functional neuronal circuitry.
Zhang
Fang
F
Developmental Biology Unit, Institute of Child Health, University College London, London, United Kingdom.
Ferretti
Patrizia
P
Clarke
Jonathan D W
JD
D000557:Ambystoma / Q000502:physiology*; D000671:Amputation, Surgical; D000818:Animals; D016922:Cellular Senescence / Q000502:physiology; D008938:Mitosis / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D011999:Recruitment, Neurophysiological / Q000502:physiology*; D013116:Spinal Cord / Q000502:physiology*; D013623:Tail / Q000294:innervation