Single multiplex polymerase chain reaction to detect diverse loci associated with diarrheagenic Escherichia coli.
We developed and tested a single multiplex polymerase chain reaction (PCR) that detects enterotoxigenic, enteropathogenic, enteroinvasive, and Shiga toxin-producing Escherichia coli. This PCR is specific, sensitive, and rapid in detecting target isolates in stool and food. Because of its simplicity, economy, and efficiency, this protocol warrants further evaluation in large, prospective studies of polymicrobial substances.
López-Saucedo
Catalina
C
Dpto. de Biomedicina Molecular, CINVESTAV-IPN, México D.F., México.
Cerna
Jorge F
JF
Villegas-Sepulveda
Nicolas
N
Thompson
Rocío
R
Velazquez
F Raul
FR
Torres
Javier
J
Tarr
Phillip I
PI
Estrada-García
Teresa
T
D017931:DNA Primers; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D029968:Escherichia coli Proteins