[Culture of endotheliocytes derived from guinea-pig common carotid artery and establishment of guinea-pig donor model].
Since endotheliocyte strains of guinea-pig and inbred strains of guinea-pig are not easily obtained, it is impossible to ensure the consistence of antigenicity between endotheliocytes and donor organs when endotheliocytes are used as an inductor in the research of immunological tolerance. So it is necessary to establish the guinea-pig endotheliocyte-donor model. One side of common carotid arteries was removed and reversed. After collagenase digestion, culture of endotheliocytes was performed. And the survival time of guinea-pig was observed. Cultured cells were confirmed as endotheliocytes by the study of morphology, immunohistochemistry and electron microscopy. The two-month-survival of guinea-pigs was 92% (103/112). The establishment of guinea-pig endotheliocyte-donor model can provide the foundation for the research of guinea-pig endotheliocyte and immunological tolerance.
Lai
Y
Y
Department of General Surgery, First Affiliated Hospital, WCUMS, Chengdu 610041.
Shen
W
W
Li
Y
Y
He
C
C
Li
S
S
D000818:Animals; D017536:Carotid Artery, Common / Q000166:cytology*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000276:immunology / Q000637:transplantation; D006168:Guinea Pigs; D007108:Immune Tolerance; D008297:Male; D014019:Tissue Donors