[The cell wall-degrading activities of lysin encoded by chlorella virus PBCV-1].
Lysin was isolated from Chlorella NC64A lysate which was infected by Chlorella virus PBCV-1. The enzyme property detection of lysin shows that it contains at least three enzyme activities: chitinase, chitosanase and beta-1, 3-Glucanase. This is consistent with the compose of Chlolrella cell wall. Chitinase and chitosanase, especially chitinase plays a important role in the process of virus entry. One 52 kD chitinase, one 56 kD chitosanase and one 36 kD chitosanase were obtained after purified by FPLC.
Han
Jigang
J
College of Life Science, Hebei Universisty, Baoding 071002, China.
Liu
Pingfang
P
Kang
Ming
M
Yie
Yin
Y
Tian
Po
P
D002473:Cell Wall / Q000201:enzymology* / Q000378:metabolism; D002688:Chitinases / Q000378:metabolism; D002708:Chlorella / Q000166:cytology / Q000201:enzymology / Q000821:virology*; D043326:Glucan 1,3-beta-Glucosidase / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D009088:Mucoproteins / Q000302:isolation & purification / Q000378:metabolism*; D017862:Phycodnaviridae / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
D009088:Mucoproteins; C010393:lysin, gastropoda; D006026:Glycoside Hydrolases; C019868:chitosanase; D002688:Chitinases; D043326:Glucan 1,3-beta-Glucosidase