title: A culture-independent survey of the bacterial community in a radon hot spring.
abstract: Paralana is an active, radon-containing hot spring situated in a region of South Australia's Flinders Ranges with a long history of hydrothermal activity. Our aim was to determine the bacterial composition of Paralana using a culture-independent, 16S rRNA-based technique. The presence of a diverse bacterial community was strongly suggested by the large number (approximately 180) of different ribotypes obtained upon analysis of nine hot spring samples. DNA sequencing of Paralana 16S rRNA genes corroborated this observation, identifying representatives of seven confirmed and two candidate divisions of the domain Bacteria. These included Cyanobacteria, Proteobacteria (both beta and delta subdivisions), the Cytophaga-Flexibacter-Bacteroides group, Low G + C Gram-positives, Nitrospira, green non-sulfur bacteria, green sulfur bacteria, OP8, and OP12. No known ionizing radiation-resistant Bacteria were identified. Only one Paralana 16S rRNA sequence type (recombinant B5D) was homologous to a sequence previously identified from a radioactive environment.
authors.affiliation: Australian Centre for Astrobiology, Department of Biological Sciences, Macquarie University, North Ryde, New South Wales, Australia. ranitori@rna.bio.mq.edu.au
authors.forename: Roberto P
authors.initials: RP
authors.lastname: Anitori
authors.forename: Cherida
authors.initials: C
authors.lastname: Trott
authors.forename: David J
authors.initials: DJ
authors.lastname: Saul
authors.forename: Peter L
authors.initials: PL
authors.lastname: Bergquist
authors.forename: Malcolm R
authors.initials: MR
authors.lastname: Walter
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D017931:DNA Primers; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011886:Radon; D013021:South Australia; D014871:Water Microbiology
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D011886:Radon