title: [Determination of aflatoxins in peanut by high performance liquid chromatography using immunoaffinity column clean-up and on-line electrochemical derivatization].
abstract: A method for the determination of aflatoxin B1, B2, G1 and G2 in peanut by high performance liquid chromatography using immunoaffinity column clean-up and on-line electrochemical derivatization was developed. Sample was extracted with 80% methanol and the extract was passed through the IAC. Aflatoxins were retained by the monoclonal in the IAC and eluted with actonitrile. AFT B1 and G1 were derivatized with on-line electrochemical equipment Kobra Cell. The four toxins can be separated in 13 minutes and the detection limit was 0.1 microgram/kg for each toxin. The method has been applied to the determination of peanut sample. The relative standard deviation was 9.2%-15% and the average recovery of AFT spiked at 0.5-9.0 micrograms/kg levels was in the range of 74.8%-97.3%.
authors.affiliation: Shandong Inport & Export Commodity Inspection Bureau, Qingdao 266002, China.
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.forename: Y B
authors.initials: YB
authors.lastname: Zhang
authors.forename: W D
authors.initials: WD
authors.lastname: Zhao
authors.forename: Y B
authors.initials: YB
authors.lastname: Li
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis; D000348:Aflatoxins / Q000032:analysis*; D010367:Arachis / Q000382:microbiology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry
chemical_list: D000348:Aflatoxins; C027955:aflatoxin G1; C029754:aflatoxin G2; C029753:aflatoxin B2; D016604:Aflatoxin B1