[Paracetamol overdose and experimental cholestasis induced gene expression regulation that codified P-Glycoprotein type 1 in rats].
Excretion of lipophilic cationic toxic compounds from the interior of the hepatocyte to the bile is mediated by P-Glycoprotein. It is an integral protein located in the bile canaliculi. The present work study the hepatic expression of P-Glycoprotein in different models of experimental liver disease: Acute paracetamol intoxication, extrahepatic cholestasis and cholestasis followed by acute paracetamol intoxication. mRNA was isolated from liver tissue, and the expression of Pg-p was assessed by Northern blot. It is concluded that the hepatic expression of mdr1b is increased in the experimental liver diseases when compared to controls. On the other hand, mdr2 expression was similar between the different groups.
Ghanem
Carolina
C
Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires.
Gomez
Paula C
PC
Arana
Cecilia
C
Bengochea
Laura
L
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000187:drug effects / Q000235:genetics*; D000082:Acetaminophen / Q000378:metabolism / Q000633:toxicity*; D000208:Acute Disease; D018712:Analgesics, Non-Narcotic / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D001646:Bile / Q000378:metabolism; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury; D001651:Cholestasis, Extrahepatic / Q000139:chemically induced* / Q000378:metabolism; D004195:Disease Models, Animal; D062787:Drug Overdose; D005786:Gene Expression Regulation / Q000187:drug effects; D019450:Genes, MDR / Q000187:drug effects / Q000235:genetics*; D022781:Hepatocytes; D008107:Liver Diseases / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018712:Analgesics, Non-Narcotic; D002352:Carrier Proteins; D012333:RNA, Messenger; D000082:Acetaminophen