title: Architecture of succinate dehydrogenase and reactive oxygen species generation.
abstract: The structure of Escherichia coli succinate dehydrogenase (SQR), analogous to the mitochondrial respiratory complex II, has been determined, revealing the electron transport pathway from the electron donor, succinate, to the terminal electron acceptor, ubiquinone. It was found that the SQR redox centers are arranged in a manner that aids the prevention of reactive oxygen species (ROS) formation at the flavin adenine dinucleotide. This is likely to be the main reason SQR is expressed during aerobic respiration rather than the related enzyme fumarate reductase, which produces high levels of ROS. Furthermore, symptoms of genetic disorders associated with mitochondrial SQR mutations may be a result of ROS formation resulting from impaired electron transport in the enzyme.
authors.affiliation: Molecular Biology Division, VA Medical Center, San Francisco, CA 94121, USA.
authors.forename: Victoria
authors.initials: V
authors.lastname: Yankovskaya
authors.forename: Rob
authors.initials: R
authors.lastname: Horsefield
authors.forename: Susanna
authors.initials: S
authors.lastname: Törnroth
authors.forename: César
authors.initials: C
authors.lastname: Luna-Chavez
authors.forename: Hideto
authors.initials: H
authors.lastname: Miyoshi
authors.forename: Christophe
authors.initials: C
authors.lastname: Léger
authors.forename: Bernadette
authors.initials: B
authors.lastname: Byrne
authors.forename: Gary
authors.initials: G
authors.lastname: Cecchini
authors.forename: So
authors.initials: S
authors.lastname: Iwata
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004140:Dinitrophenols / Q000737:chemistry / Q000494:pharmacology; D004579:Electron Transport; D042963:Electron Transport Complex II; D004926:Escherichia coli / Q000201:enzymology*; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D006418:Heme / Q000737:chemistry; D008958:Models, Molecular; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014451:Ubiquinone / Q000737:chemistry / Q000378:metabolism
chemical_list: D004140:Dinitrophenols; D009097:Multienzyme Complexes; D021122:Protein Subunits; D017382:Reactive Oxygen Species; D013481:Superoxides; D014451:Ubiquinone; D005182:Flavin-Adenine Dinucleotide; D006418:Heme; D019802:Succinic Acid; D010088:Oxidoreductases; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase