Protection against murine listeriosis by oral vaccination with recombinant Salmonella expressing protective listerial epitopes within a surface-exposed loop of the TolC-protein.
Based on the topology of the outer membrane protein TolC of Escherichia coli, a new plasmid-encoded system was created which allows the expression of antigenic peptides within permissive, surface-exposed domains of TolC. To assess the capacity of this novel antigen display system, a protective CD4 T-cell epitope of the p60 protein of Listeria monocytogenes was inserted within an extracellular loop of the TolC-protein and expressed in surface-exposed form by attenuated Salmonella enteritidis. Mice immunized orally with this recombinant S. enteritidis live vaccine strain were protected against a lethal challenge with wildtype L. monocytogenes.
Spreng
Simone
S
Department of Microbiology, University of Würzburg, D-97074, Würzburg, Germany. simone.spreng@bernabiotech.com
Dietrich
Guido
G
Goebel
Werner
W
Gentschev
Ivaylo
I
D000284:Administration, Oral; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D018985:Epitopes, B-Lymphocyte / Q000235:genetics / Q000378:metabolism; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D005455:Fluorescent Antibody Technique; D008089:Listeria monocytogenes / Q000235:genetics / Q000276:immunology; D008088:Listeriosis / Q000276:immunology / Q000517:prevention & control*; D026901:Membrane Transport Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012477:Salmonella enteritidis / Q000235:genetics / Q000378:metabolism*; D014613:Vaccines, Attenuated / Q000276:immunology
C078516:60 kDa protein, Listeria monocytogenes; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D011994:Recombinant Proteins; D014613:Vaccines, Attenuated; C073126:tolC protein, E coli