[Preparation and evaluation of alkyl ether-bonded phase for reversed-phase high performance liquid chromatography].
A new silane coupling agent, beta-(3,4-epoxycyclohexyl)ethyltrimethoxy silane, was for the firsty time used to react with octanol, then the intermediate product was coupled onto porous silica to obtain a novel bonded phase for reversed-phase high performance liquid chromatography (RP-HPLC). Characterization of prepared packing was carried out with elemental analysis, solid-state 13C nuclear magnetic resonance spectrometry (NMR), Fourier transform infrared(FT-IR) spectroscopy. Chromatographic evaluations were carried out by using a mixture of organic compounds and methanol-water as the binary mobile phase. The results showed that the stationary phase has excellent chromatographic properties and resist to hydrolysis at a pH value between 2.5 and 7.5. The silanophilic activity of the OEBP was weakened because of the existence of the cyclohexyl group in the packing. It can be efficiently used for the separation of basic compounds.
Huang
X J
XJ
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, China.
Wang
J D
JD
Liu
X L
XL
Cong
R Z
RZ
D000628:Aminophylline / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D000442:Octanols; D010208:Papaverine / Q000032:analysis; D011725:Pyridines / Q000032:analysis; D012821:Silanes; D012822:Silicon Dioxide
D000442:Octanols; D011725:Pyridines; D012821:Silanes; D000628:Aminophylline; C056178:(3,4-epoxycyclohexyl)ethyltrimethoxysilane; D012822:Silicon Dioxide; D010208:Papaverine; C023666:pyridine