[Cloning and expression of D-arabitol dehydrogenase gene from Acetobacter suboxydans in Escherichia coli].
The partial genomic library of Acetobacter suboxydans was constructed using Yeast-E. coli shuttle plasmid YEp352 as vector. Two positive transformants, designated as DH5 alpha(pAD91) and DH5 alpha(pAD98), were obtained by screening the growth of transformants on the agar plate in which D-arabitol was used as the sole carbon source. The results of Southern blot and restriction endonuclease analysis showed that the two recombinants are identical. The insert is about 2.3 kb. Arabitol dehydrogenase activity assay indicated that the transformants could produce D-xylulose-forming D-arabitol dehydrogenase. Hence, the gene encoding D-arabitol dehydrogenase exists in the cloned DNA fragment.
He
X
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Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Huai
W
W
Guo
W
W
Zhang
B
B
D000091:Acetobacter / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005822:Genetic Vectors; D015698:Genomic Library; D010957:Plasmids; D013401:Sugar Alcohol Dehydrogenases / Q000096:biosynthesis* / Q000235:genetics
D004269:DNA, Bacterial; D013401:Sugar Alcohol Dehydrogenases; C022751:D-arabinitol dehydrogenase