The binding of maize DHN1 to lipid vesicles. Gain of structure and lipid specificity.
Dehydrins (DHNs; late embryogenesis abundant D-11) are a family of plant proteins induced in response to abiotic stresses such as drought, low temperature, and salinity or during the late stages of embryogenesis. Spectral and thermal properties of these proteins in purified form suggest that they are "intrinsically unstructured." However, DHNs contain at least one copy of a consensus 15-amino acid sequence, the "K segment," which resembles a class A2 amphipathic alpha-helical, lipid-binding domain found in other proteins such as apolipoproteins and alpha-synuclein. The presence of the K segment raises the question of whether DHNs bind lipids, bilayers, or phospholipid vesicles. Here, we show that maize (Zea mays) DHN DHN1 can bind to lipid vesicles that contain acidic phospholipids. We also observe that DHN1 binds more favorably to vesicles of smaller diameter than to larger vesicles, and that the association of DHN1 with vesicles results in an apparent increase of alpha-helicity of the protein. Therefore, DHNs, and presumably somewhat similar plant stress proteins in the late embryogenesis abundant and cold-regulated classes may undergo function-related conformational changes at the water/membrane interface, perhaps related to the stabilization of vesicles or other endomembrane structures under stress conditions.
Koag
Myong-Chul
MC
Graduate Program in Biochemistry and Molecular Biology, University of California, Riverside, California 92521-0124, USA.
Fenton
Raymond D
RD
Wilkens
Stephan
S
Close
Timothy J
TJ
D000222:Adaptation, Physiological / Q000187:drug effects / Q000502:physiology; D002942:Circular Dichroism; D003080:Cold Temperature; D004190:Disasters; D050356:Lipid Metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D012639:Seeds / Q000254:growth & development; D012965:Sodium Chloride / Q000494:pharmacology; D022161:Transport Vesicles / Q000378:metabolism*; D003313:Zea mays / Q000254:growth & development / Q000378:metabolism*
D010940:Plant Proteins; C066246:late embryogenesis abundant protein, plant; C070556:dehydrin proteins, plant; D012965:Sodium Chloride