[Detection of Neisseria gonorrhoeae(NG), Chlamydia trachomatis(CT) and Ureaplasma urealyticum(UU) by multiple primer PCR].
For the detection of Neisseria gonorrhoeae(NG), Chlamydia trachomat is(CT) and Ureaplasma urealyticum(UU) in urogenital infection in one specimen, multiple primer PCR was used in 156 sex transmitted disease patients, it was shown that in 156 specimens there were 40 NG positive and 31 CT positive and 27 UU positive, 10 NG + CT positive, 4 NG + UU positive, 3 CT + UU positive and 2 NG + CT + UU positive respectively. The results were in correspondence with each other. It is suggested that multiple primer PCR is a quick, sensitive, simple and specific technique which can be applied in clinical test.
Lu
Y
Y
Department of Microbiology, Anhui Medical University, Hefei 200036.
Li
J
J
Chen
Y
Y
D000818:Animals; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D052776:Female Urogenital Diseases / Q000382:microbiology*; D008297:Male; D052801:Male Urogenital Diseases; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D015231:Sexually Transmitted Diseases, Bacterial / Q000382:microbiology*; D016990:Ureaplasma urealyticum / Q000302:isolation & purification*
D004269:DNA, Bacterial