[Study on the production of beta-mannanase by Bacillus M50].
Bacillus sp. M50 produced beta-mannanase efficiently in the medium composed of 4% konjac powder, 1.0% (NH4)2SO4 and 0.35% Na2CO3. Fermentation of beta-mannanase by Bacillus sp. M50 has been carried out in 100 L tank. The temperature, ventitation quantity and agitation speed were 30-32 degrees C, 1:0.75 vvm and 220 r/min respectively. the fermentation cycle was 28 h and the highest beta-mannanase activity was 330 u/mL. The optimal temperature and pH for beta-mannanase reaction were 50 degrees C and 6.0 respectively. It was stable at pH 4.0-7.0 and below 50 degrees C. The activity of the enzyme was strongly inhibited by Fe3+, Al3+, EDTA, Hg2+ and slightly stimulated by Ba2+, Mn2+. Treatment of ramie-fibers with crude enzyme preparations from Bacillus sp. M50 showed a good degradation for hemicellulose of ramie-fibers residues.
Chen
Y
Y
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041.
Long
J
J
Liao
L
L
Zhang
Y
Y
Yang
J
J
D001407:Bacillus / Q000201:enzymology*; D001673:Biodegradation, Environmental; D002482:Cellulose / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D044902:beta-Mannosidase / Q000096:biosynthesis* / Q000378:metabolism
D003470:Culture Media; D002482:Cellulose; D044902:beta-Mannosidase