[Listeria monocytogenes induces thymocyte apoptosis in mice].
The murine thymocyte apoptosis induced by Listeria monocytogenes(LM) was detected with morphology, FCM, and DNA electrophoresis. The results were that LM elicited typical morphological changes of thymocyte apoptosis; the typical apoptosis peak was displayed with FCM, and typical "ladder pattern" with agarose gel electrophoresis. The apoptotic cells were found at 8 h after the mice had infected LM and reached climax at 48 h. The thymus weight significantly reduced at 16 h, and reached the lowest at 48 h after the mice had infected LM. The percentage of apoptotic cells was raised with the increasing of LM. These results suggest that LM induces thymocyte apoptosis in dose- and time-dependent manner.
Chen
L Y
LY
Department of Microbiology, Xiangya School of Medicine, Central South University, Changsha 410078.
Jiang
H M
HM
Wu
G J
GJ
D000818:Animals; D017209:Apoptosis; D004247:DNA / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005260:Female; D005434:Flow Cytometry; D008089:Listeria monocytogenes / Q000502:physiology*; D051379:Mice; D013950:Thymus Gland / Q000166:cytology*
D004247:DNA