title: Induction of apoptosis by the green tea flavonol (-)-epigallocatechin-3-gallate in human endothelial ECV 304 cells.
abstract: We have previously shown that treatment with (-)-epigallocatechin-3-gallate (EGCG) inhibited vascularity and tumor growth in human colon cancer xenografts in nude mice (Jung et al: Br J Cancer 84, 2001). In this study, we examined whether endothelial cell death by EGCG is mediated by apoptosis and which molecular mechanisms are involved in this process. EGCG was found to suppress cell growth and induce apoptosis largely through mitochondrial depolarization, activation of caspase-3 and cleavage of DNA fragmentation factor-45 in human endothelial ECV 304 cells. The induction of apoptosis by EGCG was confirmed by cleaved and condensed nuclear chromatin and DNA hypoploidy. These results suggest that EGCG may exert at least part of its anticancer effect by inhibiting angiogenesis through inducing endothelial apoptosis.
authors.affiliation: Chonnam University Research Institute of Medical Sciences, Chonnam National University Medical School, Kwangju, Korea.
authors.forename: Hyeon G
authors.initials: HG
authors.lastname: Yoo
authors.forename: Boo A
authors.initials: BA
authors.lastname: Shin
authors.forename: Ju C
authors.initials: JC
authors.lastname: Park
authors.forename: Hong S
authors.initials: HS
authors.lastname: Kim
authors.forename: Won J
authors.initials: WJ
authors.lastname: Kim
authors.forename: Kee O
authors.initials: KO
authors.lastname: Chay
authors.forename: Bong W
authors.initials: BW
authors.lastname: Ahn
authors.forename: Rae K
authors.initials: RK
authors.lastname: Park
authors.forename: Lee M
authors.initials: LM
authors.lastname: Ellis
authors.forename: Young D
authors.initials: YD
authors.lastname: Jung
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D051017:Apoptosis Regulatory Proteins; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D011506:Proteins / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; D011506:Proteins; C109664:caspase-activated DNase inhibitor; D002392:Catechin; C045651:epigallocatechin gallate; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases