[Separation and determination of nycodenz and its impurities by capillary electrophoresis].
Separation and determination of nycodenz (a new radiography reagent) and its impurities by capillary electrophoresis was investigated. Since the analytes were polyols, Na2B4O7-H3BO3 was chosen as the buffer system. The analytes were negatively charged by complexing with borate. Then cetyltrimethylammonium bromide was used to reverse electroosmotic mobility in order to shorten the analysis time. 2-Propanol was added to optimize the separation further. Acceptable results of linearity, precision of corrected peak area and migration time were obtained. The RSD(n = 6) of corrected peak area and migration time were 0.73% and 1.4% respectively. The results obtained by CE were directly compared to those obtained by high performance liquid chromatography.
Lu
H J
HJ
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
Kang
J W
JW
Yang
Y T
YT
Ou
Q Y
QY
D003287:Contrast Media / Q000032:analysis / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary; D007472:Iohexol / Q000032:analysis / Q000302:isolation & purification*
D003287:Contrast Media; D007472:Iohexol