title: Specific stimulation of human apurinic/apyrimidinic endonuclease by heat shock protein 70.
abstract: We previously demonstrated the stimulation of human apurinic/apyrimidinic endonuclease 1 (HAP1) by heat shock protein 70 (HSP70). In this work, we further defined the functional interaction between these proteins. Digestion of HSP70 by trypsin released 48 and 43 kDa amino terminal fragments that retained the ability to stimulate HAP1. In agreement with this result, an HSP70 N-terminal deletion mutant protein containing amino acids 1-385 was comparable to the full-length protein in its ability to enhance HAP1 activity. HSP70 mutants containing carboxy terminal amino acids 386-640 stimulated HAP1 only slightly, as did unrelated proteins. These results implicate the amino terminal portion of HSP70 in stimulating the activity of HAP1.
authors.affiliation: Department of Radiation Oncology, Montefiore Medical Center, Albert Einstein College of Medicine, 111 East 210 Street, Bronx, NY 10467, USA.
authors.forename: Frances
authors.initials: F
authors.lastname: Mendez
authors.forename: Margarita
authors.initials: M
authors.lastname: Sandigursky
authors.forename: Raichal P
authors.initials: RP
authors.lastname: Kureekattil
authors.forename: Mark K
authors.initials: MK
authors.lastname: Kenny
authors.forename: William A
authors.initials: WA
authors.lastname: Franklin
authors.forename: Robert
authors.initials: R
authors.lastname: Bases
mesh_terms: D019757:Carbon-Oxygen Lyases / Q000378:metabolism*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009154:Mutation; D009841:Oligonucleotides / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D017434:Protein Structure, Tertiary; D014357:Trypsin / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins; D009841:Oligonucleotides; D010446:Peptide Fragments; D014357:Trypsin; D019757:Carbon-Oxygen Lyases; C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase