Selective precipitation of haptoglobin and alpha2-macroglobulin from human serum using Alocasia macrorhiza tuber protein.
Treatment of human serum with ammonium sulfate fraction (0-50%) of Alocasia macrorhiza tuber extract resulted in precipitation at neutral pH. The precipitate was dissolved at pH 10.5 and chromatographed on Sephadex G-100 column. Two protein peaks were resolved. While the first peak represented alpha2-macroglobulin and haptoglobin, the second peak accounted for specific Alocasia protein. Incidentally the Alocasia protein was shown to be responsible for selective and specific precipitation of alpha2-macroglobulin and haptoglobin from serum. Thus the plant protein in its pure form or in crude stage could be used for the rapid isolation of two of the prominent alpha2-globulins.
Nayak
B Shivananda
BS
Department of Biochemistry, Kasturba Medical College, Manipal-576 119, India. drbsraj@yahoo.co.in
Ulloor
N Jagadish
NJ
Shivaraj
B
B
D031055:Alocasia / Q000378:metabolism*; D000510:Alpha-Globulins / Q000302:isolation & purification / Q000378:metabolism; D001769:Blood; D001774:Blood Chemical Analysis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006242:Haptoglobins / Q000302:isolation & purification*; D006801:Humans; D000511:alpha-Macroglobulins / Q000302:isolation & purification*
D000510:Alpha-Globulins; D006242:Haptoglobins; D000511:alpha-Macroglobulins