title: Characterization of KIBRA, a novel WW domain-containing protein.
abstract: In a yeast two hybrid screen with the human isoform of Dendrin (KIAA0749), a putative modulator of the postsynaptic cytoskeleton, we isolated a cDNA coding for a novel protein, KIBRA, possessing two amino-terminal WW domains, an internal C2-like domain and a carboxy-terminal glutamic acid-rich stretch. Northern blot analysis revealed that the expression of KIBRA mRNA was predominately found in kidney and brain. In vitro interaction studies revealed that the first KIBRA WW domain binds specifically to PPxY motifs. Transient transfection of monkey kidney cells with constructs encoding Myc-tagged KIBRA displayed a cytoplasmic localization and a perinuclear enrichment of the protein.
authors.affiliation: Department for Experimental Tumorbiology, University Muenster, Badestrasse 9, D-48149 Muenster, Germany. kremers@uni-muenster.de
authors.forename: Joachim
authors.initials: J
authors.lastname: Kremerskothen
authors.forename: Christian
authors.initials: C
authors.lastname: Plaas
authors.forename: Katrin
authors.initials: K
authors.lastname: Büther
authors.forename: Indra
authors.initials: I
authors.lastname: Finger
authors.forename: Stefan
authors.initials: S
authors.lastname: Veltel
authors.forename: Theodoros
authors.initials: T
authors.lastname: Matanis
authors.forename: Thomas
authors.initials: T
authors.lastname: Liedtke
authors.forename: Angelika
authors.initials: A
authors.lastname: Barnekow
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010750:Phosphoproteins; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; C513637:WWC1 protein, human; C104328:dendrin