Comparison of Salmonella enterica serovar Abortusequi isolates of equine origin by pulsed-field gel electrophoresis and fluorescent amplified-fragment length polymorphism fingerprinting.
Equine paratyphoid is caused by Salmonella enterica serovar Abortusequi, and manifests mainly as abortion in the mare. We compared S. Abortusequi strains isolated in Japan and other countries using pulsed-field gel electrophoresis (PFGE) and fluorescent amplified-fragment length polymorphism (FAFLP) analysis. PFGE analysis of S. Abortusequi strains gave 21-27 fragments ranging in size from 33 to 602kb. Although two PFGE profiles were observed among the 20 S. Abortusequi isolates in Japan, the restriction fragments originating from the chromosome were common between the two profiles. The similarity index of the two profiles was 90.9%, while those between Japanese and five other S. Abortusequi strains were 29.8-37.5%. On the other hand, FAFLP analysis of S. Abortusequi strains generated 64-67 amplified fragments ranging in size from 100 to 400bp. One polymorphic fragment was observed among the 20 S. Abortusequi isolates in Japan. These data indicate the close relation of this agent in Japan. S. Abortusequi strains sharing a common ancestry might have been conserved in Japan.
Akiba
Masato
M
Kyusyu Research Station, National Institute of Animal Health, 2702 Chuzan-cho, Kagoshima-shi, Japan. akiba@affrc.go.jp
Uchida
Ikuo
I
Nishimori
Kei
K
Tanaka
Kiyoshi
K
Anzai
Toru
T
Kuwamoto
Yasushi
Y
Wada
Ryuichi
R
Ohya
Tatsuo
T
Ito
Hiroya
H
D000818:Animals; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005260:Female; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D007564:Japan; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D019779:Salmonella enterica / Q000737:chemistry / Q000235:genetics*
D004269:DNA, Bacterial