title: [Sequencing and analysis of flocculation gene(FLO1G)].
abstract: The sequence of the flocculation gene (FLO1G) was determined. The result of sequcencing showed that: the cloned gene contains a large open reading frame (ORF) of 3936 bp and encodes for a protein of 1312 amino acid. According to the result of homologous analysis, the cloned gene is homologous to FLO1 but with 675 bp deletion in the ORF region. The missing part belongs to one of the four repeated sequence family of FLO1. Since the cloned DNA fragment can trigger strong flocculence to non-flocculent strain S. cerevisiae YS58, we concluded that the missing part is not the crutical part for the flocculent ability of the gene.
authors.affiliation: Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: He
authors.forename: Wenjie
authors.initials: W
authors.lastname: Guo
authors.forename: Borun
authors.initials: B
authors.lastname: Zhang
authors.forename: Pei
authors.initials: P
authors.lastname: Zhang
authors.forename: Gang
authors.initials: G
authors.lastname: Lian
authors.forename: Chenghong
authors.initials: C
authors.lastname: Wang
authors.forename: Xueqi
authors.initials: X
authors.lastname: Pan
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics*; D005427:Flocculation; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D037241:Mannose-Binding Lectins; D016366:Open Reading Frames; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; C087578:FLO1 protein, S cerevisiae; D005656:Fungal Proteins; D037241:Mannose-Binding Lectins; D029701:Saccharomyces cerevisiae Proteins