[Molecular cloning and expression in E. coli of the surface-exposed lipoprotein LipL41 gene of Leptospira lai].
OBJECTIVE
To construct a recombinant expressive plasmid using LipL41 gene of leptospira lai for further research of subunit vaccine of leptospira.


METHODS
A pair of oligonucleotide primers were designed based on the sequence of LipL41 of leptospira kirschneri RM 52 in Genbank. With genomic DNA of leptospira lai 017 as template, a fragment was amplified by PCR and DNA sequencing analysis showed this fragment to be the gene that encodes LipL41. A recombinant vector was constructed using plasmid pGEX1 lambda T and the expression of LipL41 gene was tested.


RESULTS
The production of PCR was LipL41 gene. The recombinant plasmid was constructed and testified by nuclease digestion and PCR, and LipL41 gene could express in E. coli.


CONCLUSIONS
The recombinant plasmid has been constructed successfully and LipL41 gene can express stably at high level.
Li
X
X
Research Unit of Infection, School of Basic Medical Sciences, WCUMS, Chengdu 610041, China.
Bao
L
L
Hu
C
C
Xie
Y
Y
Yan
J
J
Zhang
H
H
D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D007921:Leptospira interrogans / Q000145:classification / Q000235:genetics*; D008049:Lipase / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D011994:Recombinant Proteins; D008049:Lipase; C120013:lactonizing lipase protein, Pseudomonas