title: Role of Ets-1 in erythroid differentiation.
abstract: Members of the Ets gene family are known to be expressed in the hematopoietic tissue and some of them play a pivotal role in normal hematopoietic cell development. Ets-1 gene expression was analyzed in Friend Leukemia Cells (FLC) induced to erythroid differentiation by DMSO. We show that the level of Ets-1 protein and its binding activity decreases in FLC along erythroid differentiation of primary human progenitors. The same behavior was observed during normal erythroid differentiation. Moreover, FLC constitutively expressing Ets-1 show a decrease in TfR gene expression, globin mRNA and hemoglobin synthesis. These data indicate that a decrease in Ets-1 binding activity is required for a normal erythroid maturation and that a deregulated expression of this transcription factor may interfere with terminal erythroid differentiation.
authors.affiliation: Laboratory of Virology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Marziali
authors.forename: Edvige
authors.initials: E
authors.lastname: Perrotti
authors.forename: Ramona
authors.initials: R
authors.lastname: Ilari
authors.forename: Valentina
authors.initials: V
authors.lastname: Lulli
authors.forename: Eliana M
authors.initials: EM
authors.lastname: Coccia
authors.forename: Simona
authors.initials: S
authors.lastname: Mazzeo
authors.forename: Lukas C
authors.initials: LC
authors.lastname: Kühn
authors.forename: Ugo
authors.initials: U
authors.lastname: Testa
authors.forename: Angela
authors.initials: A
authors.lastname: Battistini
mesh_terms: D002454:Cell Differentiation / Q000502:physiology*; D015672:Erythroid Precursor Cells / Q000502:physiology*; D006801:Humans; D020014:K562 Cells; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: C494041:ETS1 protein, human; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors