Investigation of the complex descending innervation of the dorsal cochlear nucleus in the rat: a transneuronal tract-tracing study using pseudorabies virus.
The afferent neuronal connections of the dorsal cochlear nucleus were investigated in rats by using a trans-synaptic retrograde tract-tracing method. The neurotropic viral tracer, the Bartha strain of the pseudorabies virus was stereotaxically injected into the dorsal cochlear nucleus, ipsilaterally. Neurons, which project directly or indirectly (one or multiple relays by other neurons) to the dorsal cochlear nucleus were infected and visualized by immunohistochemistry. Labeled neurons were found in each components of the auditory pathway, some of the monoaminergic cell groups in the lower brainstem, the hypothalamus and in some limbic areas.
Helfferich
Frigyes
F
Department of Otolaryngology, National Healthcare Center, Szabolcs utca 33-35, 1138, Budapest, Hungary.
Uhereczky
Gabriella
G
Boldogkoi
Zsolt
Z
Vitéz
Lajos Csokonai
LC
Palkovits
Miklós
M
D000818:Animals; D001306:Auditory Pathways / Q000166:cytology*; D001933:Brain Stem / Q000166:cytology / Q000821:virology; D017626:Cochlear Nucleus / Q000166:cytology / Q000821:virology*; D011558:Herpesvirus 1, Suid / Q000502:physiology*; D007031:Hypothalamus / Q000166:cytology / Q000821:virology; D007150:Immunohistochemistry; D008032:Limbic System / Q000166:cytology / Q000821:virology; D008297:Male; D009474:Neurons / Q000166:cytology; D051381:Rats; D011921:Rats, Inbred WKY