Surface imprinting polymers for the recognition of nucleotides.
A highly selective polymer has been prepared for the selective separation of nucleotides by the surface imprinting polymerization. A dialkyl quaternary ammonium chloride was effective as the functional molecule for recognizing the difference in the structure of nucleotides. Adsorptive behavior of the ionic species of the structural analogues, inosine-5'-monophosphoric acid (IMP) and guanosine-5'-monophosphoric acid (GMP), could be controlled by changing the pH condition. Surface imprinting polymers were prepared under different pH conditions; pH 9.0 and pH 8.5. The IMP-imprinted polymers exhibited higher template effect for IMP than for a structural analogue, GMP. A reference polymer prepared without the imprint molecule neither exhibit any selectivity to IMP nor to GMP. The adsorption behavior was quantitatively evaluated by the binding constants for the IMP-imprinted polymer. The imprinting polymer was found to recognize a small structural difference in nucleotides.
Tsunemori
H
H
Department of Chemical Systems and Engineering, Graduate School of Engineering, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan.
Araki
K
K
Uezu
K
K
Goto
M
M
Furusaki
S
S
D000327:Adsorption; D001665:Binding Sites; D006157:Guanosine Monophosphate / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007291:Inosine Monophosphate / Q000737:chemistry; D009711:Nucleotides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
D009711:Nucleotides; D011108:Polymers; D007291:Inosine Monophosphate; D006157:Guanosine Monophosphate