[Activation of NF-kappa B induced by stretching and the gene expression of beta-MHC regulated by NF-kappa B in cardiac myocytes].
OBJECTIVE
To determine the mechanism of reexpression of beta-MHC gene caused by the stimulation of stretch.


METHODS
The specimens were taken from the 1-, 2- and 3-day old SD rats. Immunohistochemical method was applied to observe the activation of NF-kappa B in stretched cardiac myocytes cultured on the silicon membrane, and then with the system of image analysis, the beta-MHC protein content was detected in four groups where NF-kappa B was activated or not activated, respectively.


RESULTS
NF-kappa B was activated significantly by stretching cardiac myocytes for five hours. The beta-MHC protein content in stretched myocytes increased significantly, compared with that in the control myocytes, NAC-treated myocytes, and stretch-treated myocytes in the presence of NAC.


CONCLUSIONS
The above data demonstrate that NF-kappa B signaling system is involved in regulating the stretch-induced gene expression of beta-MHC.
Liu
R
R
Biomedical Engineering Department, School of Basic Medical Sciences, WCUMS, Chengdu 610041, China.
Yin
H
H
Wang
Z
Z
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D008465:Mechanoreceptors / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000096:biosynthesis* / Q000235:genetics; D016328:NF-kappa B / Q000378:metabolism*; D024501:Nonmuscle Myosin Type IIB; D051381:Rats; D017207:Rats, Sprague-Dawley; D012026:Reflex, Stretch
D016328:NF-kappa B; D024501:Nonmuscle Myosin Type IIB; C103815:nonmuscle myosin type IIB heavy chain; D018995:Myosin Heavy Chains