The receptor-bound N-terminal ectodomain of the amyloid precursor protein is associated with membrane rafts.
The soluble N-terminal ectodomain of amyloid precursor protein (sAPP), resulting from alpha-secretase-mediated proteolytic processing, has been shown to function as a growth factor for epithelial cells, including keratinocytes and thyrocytes. Extracellularly applied sAPP binds to a cell surface receptor and exhibits a patchy binding pattern reminiscent of that observed for raft proteins. Here we show that (i) the receptor-bound sAPP resides in a detergent-insoluble membrane microdomain which cofractionates in density gradients with cholesterol-rich membrane rafts and caveolae; (ii) the sAPP-binding microdomains are different from caveolae; and (iii) sAPP is capable of binding to isolated rafts and inducing tyrosine phosphorylation of some raft proteins. These observations suggest that a novel type of membrane raft is involved in sAPP signaling.
Tikkanen
Ritva
R
Institute of Cell Biology and Bonner Forum Biomedizin University of Bonn, Ulrich-Haberland Strasse 61a, D-53121 Bonn, Germany
Icking
Ann
A
Beicht
Peter
P
Waneck
Gerald L
GL
Volker
Herzog
H
D016564:Amyloid beta-Protein Precursor / Q000096:biosynthesis* / Q000737:chemistry; D000818:Animals; D021941:Caveolae / Q000378:metabolism; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D003902:Detergents; D004705:Endocytosis / Q000502:physiology; D005347:Fibroblasts / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015603:Keratinocytes / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D021962:Membrane Microdomains / Q000737:chemistry / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D010766:Phosphorylation; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012995:Solubility; D014443:Tyrosine / Q000378:metabolism
D016564:Amyloid beta-Protein Precursor; D003902:Detergents; D011994:Recombinant Proteins; D014443:Tyrosine