[Analysis of headspace constituents of Gardenia flower by GC/MS with solid-phase microextraction and dynamic headspace sampling].
The headspace constituents of fresh Gardenia flower were investigated by GC/MS. The headspace volatiles were sampled by solid-phase microextraction (SPME) and dynamic headspace sampling (DHS). SPME sampling was conducted with 100 microns PDMS fiber at 28 degrees C for 60 min. In DHS sampling, purified nitrogen was used as purging gas with a flow rate at 80 mL/min for 120 min. Tenax GR(20 mesh-40 mesh) was used as adsorbent and the volatiles were eluted by ether, and concentrated to 0.5 mL for GC/MS analysis. A Supelco-wax capillary column (30 m x 0.25 mm i.d. x 0.25 micron df) was employed in GC/MS analysis. Initial oven temperature was kept at 45 degrees C for 2 min, then raised to 250 degrees C at 4 degrees C/min, and kept at 250 degrees C for 10 min. According to SPME-GC/MS analysis, the main compounds in headspace of fresh Gardenia flower included farnesene(64.86%), cis-ocimene(29.33%), linalool(2.74%), cis-3-hexenyl tiglate(1.34%), methyl benzoate(0.25%). Results obtained from SPME and DHS sampling were also compared. In this study, SPME afforded a simpler and more sensitive sampling method, and much more accurate information about headspace volatiles of Gardenia flower.
Liu
B Z
BZ
Analytical Center, Hefei Institute of Economic & Technology, Hefei 230052, China.
Gao
Y
Y
D000080462:Acyclic Monoterpenes; D001565:Benzoates / Q000032:analysis; D002849:Chromatography, Gas; D035264:Flowers / Q000737:chemistry*; D032062:Gardenia / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D039821:Monoterpenes / Q000032:analysis*; D014835:Volatilization
D000080462:Acyclic Monoterpenes; D001565:Benzoates; D039821:Monoterpenes; C044605:methyl benzoate; C018584:linalool