[The detection and clinical significance of telomerase activity in supraglottic carcinomas].
OBJECTIVE
To study the expression and significance of telomerase activity in laryngeal carcinoma.


METHODS
We detected telomerase activity of HEP-2 cell line, 26 cases of supraglottic carcinoma tissues and 15 cases of peri-carcinoma tissues with PCR-based assay designated TRAP (for telomeric repeat amplification protocol).


RESULTS
The telomerase positive rate of supraglottic carcinoma 84.6% (22/26) was obvious higher than that of peri-carcinoma 40% (6/15), P < 0.01. No significant differences in positive rates were found among different groups divided according to clinical stage or metastasis of cervical lymph node, P > 0.05.


CONCLUSIONS
Telomerase activation is related to immortalization process, though not much helpful in diagnosis of supraglottic carcinoma.
Wei
H
H
Department of Otorhinolaryngology, the First Affiliated Hospital, China Medical University, Shenyang 110001.
Guo
X
X
Fu
W
W
Sun
K
K
D000328:Adult; D000368:Aged; D005260:Female; D005931:Glottis / Q000201:enzymology*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D012091:Repetitive Sequences, Nucleic Acid; D019098:Telomerase / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D019098:Telomerase