[Simultaneous analysis of histamine and noradrenaline in human plasma by high performance liquid chromatography/electrochemical detection].
This paper reports a reversed-phase high performance liquid chromatographic method for the simultaneous separation and determination of histamine(Hist) and noradrenaline(NA) in human plasma by means of pre-column derivatization of Hist and NA with OPA in the presence of 2-mercaptoethanol. The analysis was carried out with Partisil ODS column, V[sodium acetate buffer solution(pH 4.90)]:V(acetonitrile) = 7:3 as mobile phase and 3-methylhistamine as internal standard. The detector potential was +0.7 V for both compounds to keep minimum background noise. A linear relationship between the electrochemical signal and the compound mass concentrations was obtained. The minimum detectable limit was 15 ng/L (S/N = 3). An example of application of the method to the determination of Hist and NA in human plasma is presented.
Liu
W
W
Central Lab, Xinxiang Medical College, Xinxiang, 453003.
Qi
W
W
Zheng
H
H
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D006632:Histamine / Q000097:blood*; D006801:Humans; D008623:Mercaptoethanol; D009638:Norepinephrine / Q000097:blood*
D008623:Mercaptoethanol; D006632:Histamine; D009638:Norepinephrine