[Effect of Astragalus membranaceus injection on megakaryocyte hematopoiesis in anemic mice].
OBJECTIVE
To assess the effect of Astragalus membranaceus injection (AMI) on megakaryocyte hematopoiesis in anemic mice and explore its mechanism.


METHODS
Anemic models of mice were randomly divided into two groups: treatment group and anemic control group. Intraperitoneal doses of AMI (20 mg/(ml.20 g) q.d x 6) were given to the treatment group, and equal doses of physiological saline were given to the anemic control group. On days 8, 11 and 14 after treatment, blood platelet and bone marrow cells were determined, and the numbers of CFU-Meg (colony forming unit-megakaryocyte) and Meg-CSA (megakaryocyte colony-stimulating activity) were determined by using technique of hematopoietic progenitor cells culture in vitro.


RESULTS
Serum Meg-CSA of the treatment group was significantly higher than that of the anemic control group. The abovementioned indices of the treatment group recovered to normal by day 11, which was markedly earlier than the day of recovery observed in the anemic control group.


CONCLUSIONS
AMI can increase serum Meg-CSA of anemic mice and accelerate the recovery of megekaryocyte hematopoiesis after bone marrow suppression.
Zhu
X
X
Department of Histology and Embryology, School of Basic Medical Sciences, WCUMS, Chengdu 610041, China.
Zhu
B
B
D000741:Anemia, Aplastic / Q000097:blood* / Q000139:chemically induced; D000818:Animals; D027883:Astragalus Plant; D027885:Astragalus propinquus / Q000737:chemistry*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000473:pathology; D008533:Megakaryocytes / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010976:Platelet Count; D011897:Random Allocation
D004365:Drugs, Chinese Herbal; C027492:Huang Qi