Adrenomedullin stimulates the growth of cultured normal human osteoblasts as an autocrine/paracine regulator.
Adrenomedullin (AM) is a 52 amino acid peptide that is synthesized in a variety of tissues, including the vessels and bones. This study showed that normal human osteoblast (NHOst) secreted immunoreactive AM and that AM stimulated intracellular cAMP production in these cells. An anti-AM monoclonal antibody, which inhibited endogenous AM, caused the number of NHOst to decrease. The effect of a low concentration AM was inhibited by addition of a cAMP-dependent protein kinase A inhibitor (H89). These data suggest that AM is an autocrine or paracrine regulator that promotes the proliferation of NHOst via the cAMP pathway.
Hamada
H
H
Department of Orthopedic Surgery, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. hiroakI@fc.miyazaki-med.ac.jp
Kitamura
K
K
Chosa
E
E
Eto
T
T
Tajima
N
N
D053607:Adrenomedullin; D000911:Antibodies, Monoclonal / Q000276:immunology; D019898:Autocrine Communication / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D010006:Osteoblasts / Q000378:metabolism*; D019899:Paracrine Communication / Q000502:physiology*; D010455:Peptides / Q000276:immunology / Q000378:metabolism*; D011863:Radioimmunoassay; D013997:Time Factors
D000911:Antibodies, Monoclonal; D010455:Peptides; D053607:Adrenomedullin; D000242:Cyclic AMP