Risk factors for Kaposi's sarcoma in men seropositive for both human herpesvirus 8 and human immunodeficiency virus.
OBJECTIVE
To identify risk factors for Kaposi's sarcoma (KS) among men seropositive for both human herpesvirus 8 (HHV-8) and HIV.


METHODS
Cross-sectional study of 91 HHV-8 seropositive, HIV seropositive men who have sex with men (57 with KS), and 70 controls at lower risk for KS.


METHODS
Patients received clinical evaluations. Blood, oral fluids, semen, rectal brush, rectal swab, and urine were collected, and tests for HHV-8 were performed.


RESULTS
Men with KS were more likely to have HHV-8 DNA in peripheral blood mononuclear cells (PBMC) than men without KS [35.1 versus 5.9%, odds ratio (OR), 8.6, 95% confidence interval (CI), 1.9-39.9]. The prevalence of HHV-8 DNA in oral fluids was similar for the two groups (37.0 versus 32.4%; OR, 1.2; 95% CI, 0.5-3.0). HHV-8 DNA was rarely detected in specimens of other types from these men, or in any specimens from the 70 controls. Among men with KS, HHV-8 DNA in PBMC was associated with new KS lesions (OR, 4.5; 95% CI, 1.4-14.5), and HHV-8 DNA in oral fluids was associated with oropharyngeal KS lesions (OR, 3.1; 95% CI, 1.0-10.1). Men with high HHV-8 antibody titers were more likely to have KS (OR, 9.6; 95% CI, 1.2-78.2), but were less likely to have new KS lesions (OR, 0.2; 95% CI, 0.0-1.1) or HHV-8 DNA in PBMC (OR, 0.2; 95% CI, 0.0-1.6) or oral fluids (OR, undefined; = 0.001).


CONCLUSIONS
In HHV-8- and HIV-seropositive men, HHV-8 DNA is associated with KS. Among men without KS, HHV-8 DNA is most commonly found in oral fluids. High HHV-8 antibody titers may protect against circulating HHV-8 and new KS lesions.
Cannon
Michael J
MJ
Emory University, Atlanta, Georgia, USA. mcannon@cdc.gov
Dollard
Sheila C
SC
Black
Jodi B
JB
Edlin
Brian R
BR
Hannah
Connie
C
Hogan
Susan E
SE
Patel
Mitesh M
MM
Jaffe
Harold W
HW
Offermann
Margaret K
MK
Spira
Thomas J
TJ
Pellett
Philip E
PE
Gunthel
Clifford J
CJ
D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000276:immunology; D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D018791:CD4 Lymphocyte Count; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000097:blood; D015658:HIV Infections / Q000150:complications* / Q000276:immunology; D019288:Herpesvirus 8, Human / Q000276:immunology / Q000302:isolation & purification*; D018451:Homosexuality, Male; D006801:Humans; D008297:Male; D016017:Odds Ratio; D012307:Risk Factors; D012514:Sarcoma, Kaposi / Q000276:immunology / Q000821:virology*; D019562:Viral Load
D000914:Antibodies, Viral; D004279:DNA, Viral