Electrophoretic mobilities and migrating analytes: Part 1: Relationships.
The molecular radii (r) of a series of peptides have been determined by molecular modeling. With these data, it is shown that electrophoretic mobility (mu(ep)) is proportional to 1/r2, and that the dependence presented in textbooks (mu(ep) infinity 1/r) is wrong. Use of the approximately equivalent, mass-based Offord equation is discussed, and other relevant considerations are presented.
Cross
Reginald F
RF
School of Engineering and Science, Environment & Biotechnology Centre, Swinburne University of Technology, Hawthorn, Victoria, Australia. rcross@swin.edu.au
Wong
Margaret G
MG
D019075:Electrophoresis, Capillary / Q000379:methods*; D008433:Mathematics; D008956:Models, Chemical; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification
D010455:Peptides