title: [Identification and location of the toxin protein genes in 56 Bacillus thuringiensis isolates].
abstract: Polymerase chain reaction was performed to screen Bacillus thuringiensis isolates for cryI, cryIII, and cryV genes with cryI-, cryIII-, and cryV-specific primers respectively. The results indicated that, in the 56 Bt isolates examined, 7 isolates contain cryI gene, 2 isolates contain cryIII gene, and 21 isolates contain both cryI and cryV genes. The cryV gene was located by Southern hybridization in the large plasmid of about 150 MD molecular mass.
authors.affiliation: Institute of Entomology and State Key Laboratory for Biocontrol, Zhongshan University, Guangzhou 510275, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhong
authors.forename: M
authors.initials: M
authors.lastname: Yuan
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.forename: R
authors.initials: R
authors.lastname: Deng
authors.forename: Q
authors.initials: Q
authors.lastname: Long
authors.forename: Y
authors.initials: Y
authors.lastname: Pang
authors.forename: X
authors.initials: X
authors.lastname: Wang
mesh_terms: D001413:Bacillus thuringiensis / Q000737:chemistry / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D001427:Bacterial Toxins / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004731:Endotoxins / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D010957:Plasmids; D016133:Polymerase Chain Reaction
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D017931:DNA Primers; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis