Construction and in vitro expression of Streptococcus mutans surface protein encoding DNA vaccine.
DNA vaccine plasmids were constructed that encoded two highly-conservative regions of a surface protein, PAc, from the human major cariogenic bacterium, Streptococcus mutans. Antigen expression was evaluated in vitro by immunohistochemical analysis of human endothelial cells following cationic liposome-mediated transient transfection with recombinant plasmid. The results of this study provided a basis for further testing of these recombinant plasmids in primates and for efficacy testing of dental caries DNA vaccines in human volunteers in future.
Peng
Z
Z
Stomatological Center of Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030.
Fan
M
M
Bian
Z
Z
Chen
Z
Z
Peng
B
B
D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D004269:DNA, Bacterial; D003731:Dental Caries / Q000517:prevention & control; D000939:Epitopes; D006801:Humans; D008562:Membrane Glycoproteins; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D022541:Streptococcal Vaccines; D013295:Streptococcus mutans / Q000276:immunology*; D019444:Vaccines, DNA
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D000939:Epitopes; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; C055764:S-layer proteins; D022541:Streptococcal Vaccines; D019444:Vaccines, DNA