title: [Separation of echinacoside by reversed-phase preparative high performance liquid chromatography].
abstract: Echinacoside, one kind of phenylethanoid glycosides (PhGs), was isolated from the stems of Cistanche tubulosa (Schenk) R. Wight with a series of steps, including solvent extraction, D101 polymer adsorption column separation, Sephadex LH-20 separation, C18 column reversed-phase preparative high performance liquid chromatographic (RP-prep-HPLC) preparation and polyamide thin-layer chromatographic detection. The purity of the product was over 98%. The chemical structure of echinacoside was identified by 1H NMR and 13C NMR. To find out the optimum condition of mobile phase of RP-prep-HPLC, several systems were used in this work. Finally acetonitrile-1% HCOOH water (18:82, V/V) system was found to be the best. On the other hand D101 polymer adsorption column and Sephadex LH-20 were also effective for PhGs separation.
authors.affiliation: Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
authors.forename: L
authors.initials: L
authors.lastname: Lei
authors.forename: Z H
authors.initials: ZH
authors.lastname: Song
authors.forename: P F
authors.initials: PF
authors.lastname: Tu
authors.forename: L J
authors.initials: LJ
authors.lastname: Wu
authors.forename: F K
authors.initials: FK
authors.lastname: Chen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031671:Cistanche / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D018547:Plant Stems / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D006027:Glycosides; C060297:echinacoside