Granulicatella adiacens and Abiotrophia defectiva bacteraemia characterized by 16S rRNA gene sequencing.
Traditionally, the identification, epidemiology and spectrum of clinical diseases caused by Granulicatella adiacens and Abiotrophia defectiva are dependent upon their phenotypic characterization. During a 6-year period (July 1995-June 2001), seven and two alpha-haemolytic streptococci were identified as G. adiacens and A. defectiva, respectively, by 16S rRNA gene sequencing. Three patients with haematological malignancies and neutropenic fever had primary bacteraemia. Three patients with valvular problems or congenital heart disease had infective endocarditis. A patient with ischemic heart disease and cerebrovascular accident had infected aortic atheroma with dissection. A patient with recurrent pyogenic cholangitis had acute cholangitis and a patient with polypoid cystitis and benign prostatic hypertrophy had acute prostatitis. Four of the nine patients died, including all three with G. adiacens infective endocarditis or infected atheroma. For the seven G. adiacens isolates, the API 20 STREP system successfully identified one and five isolates as G. adiacens with >95 % and 80-90 % confidence, respectively, whereas the Vitek System (GPI) and ATB Expression system (ID32 STREP) successfully identified none and one isolate as G. adiacens. Of the two A. defectiva isolates, none of the three systems successfully identified either of them as A. defectiva. 16S rRNA gene sequencing is the technique of choice for identifying G. adiacens and A. defectiva, and early surgical intervention should be considered when G. adiacens endocarditis is diagnosed.
Woo
Patrick Chiu-Yat
PC
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong 2Department of Microbiology, Tuen Mun Hospital, Hong Kong 3Department of Microbiology, Pamela Youde Nethersole Eastern Hospital, Hong Kong 4HKU-Pasteur Research Centre, Hong Kong.
Fung
Ami Mei-Yuk
AM
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong 2Department of Microbiology, Tuen Mun Hospital, Hong Kong 3Department of Microbiology, Pamela Youde Nethersole Eastern Hospital, Hong Kong 4HKU-Pasteur Research Centre, Hong Kong.
Lau
Susanna Kar-Pui
SK
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong 2Department of Microbiology, Tuen Mun Hospital, Hong Kong 3Department of Microbiology, Pamela Youde Nethersole Eastern Hospital, Hong Kong 4HKU-Pasteur Research Centre, Hong Kong.
Chan
Benedict Yin-Leung
BY
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong 2Department of Microbiology, Tuen Mun Hospital, Hong Kong 3Department of Microbiology, Pamela Youde Nethersole Eastern Hospital, Hong Kong 4HKU-Pasteur Research Centre, Hong Kong.
Chiu
Siu-Kau
SK
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong 2Department of Microbiology, Tuen Mun Hospital, Hong Kong 3Department of Microbiology, Pamela Youde Nethersole Eastern Hospital, Hong Kong 4HKU-Pasteur Research Centre, Hong Kong.
Teng
Jade Lee-Lee
JL
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong 2Department of Microbiology, Tuen Mun Hospital, Hong Kong 3Department of Microbiology, Pamela Youde Nethersole Eastern Hospital, Hong Kong 4HKU-Pasteur Research Centre, Hong Kong.
Que
Tak-Lun
TL
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong 2Department of Microbiology, Tuen Mun Hospital, Hong Kong 3Department of Microbiology, Pamela Youde Nethersole Eastern Hospital, Hong Kong 4HKU-Pasteur Research Centre, Hong Kong.
Yung
Raymond Wai-Hung
RW
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong 2Department of Microbiology, Tuen Mun Hospital, Hong Kong 3Department of Microbiology, Pamela Youde Nethersole Eastern Hospital, Hong Kong 4HKU-Pasteur Research Centre, Hong Kong.
Yuen
Kwok-Yung
KY
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong 2Department of Microbiology, Tuen Mun Hospital, Hong Kong 3Department of Microbiology, Pamela Youde Nethersole Eastern Hospital, Hong Kong 4HKU-Pasteur Research Centre, Hong Kong.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016470:Bacteremia / Q000175:diagnosis / Q000382:microbiology*; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000737:chemistry; D004697:Endocarditis, Bacterial / Q000175:diagnosis / Q000382:microbiology; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000175:diagnosis / Q000382:microbiology*; D013291:Streptococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S