[A novel method for preparation of C18 ester-bond RP-HPLC packing].
RP-HPLC packings have found widespread application in the fields of biology, chemistry and pharmaceutical industry, etc. C18-bonded phase is one of the most important packing. In order to avoid using toxic and unstable chloro-containing silane as derivatization reagent, a new practical and economic procedure had been developed to prepare RP packing. Octadecyl group was bound onto YQG80, 5 microns silica (Tsingdao Oceanic Chemical Factory, particle size d.p. 5 microns) with gamma-glycidoxypropyltrimethoxysilane as coupling agent. Elemental analysis and chromatographic evaluation confirmed the feasibility of the procedure and RP behavior of the packing. After eluting with 500 column volume of mobile phase at different pH (2, 5 and 8), the column still showed good performance (> 64,000 theoretical plates/m) and stability.
Yu
X
X
Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Zhao
R
R
Liu
G Q
GQ
D002244:Carbon; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D004952:Esters; D010316:Particle Size; D012821:Silanes; D012822:Silicon Dioxide
D004952:Esters; D012821:Silanes; C039462:gamma-glycidoxypropyltrimethoxysilane; D002244:Carbon; D012822:Silicon Dioxide; C042953:octadecyl silane-bonded silica