title: Subcellular identification of angiotensin I/II- and angiotensin II (AT1)-receptor-immunoreactivity in the central nervous system of rats.
abstract: To gain insight into generating and transport mechanisms of angiotensin (ANG) in the brain the study was focused on the subcellular localization of ANG II and its AT(1)-receptors in the hypothalamus of rats. The present paper demonstrates ANG II- and AT(1)-receptor-immunolabelling at brain parenchyma vessels and at glial and neuronal structures in the perivascular region. Further, ANG II- and AT(1)-receptor-immunoreactivity is shown at plasma membranes and intracellular structures in the ependyma of the third ventricle. Based upon a conventional horseradish peroxidase technique, combined with the classical substrate 3,3'-diaminobenzidine, a procedure is introduced that will be useful with a variety of antibodies used on glutar- and paraformaldehyde-fixed brain tissue. This technique enables a fast correlation between light and electron microscopical results and might also provide an attractive alternative to colloidal gold-labelling and silver-intensification techniques.
authors.affiliation: Institute of Pharmacology and Toxicology, Ruprecht-Karls University of Heidelberg, Heidelberg, Germany. mthomas@stud.uni-frankfurt.de
authors.forename: Martin Alexander
authors.initials: MA
authors.lastname: Thomas
authors.forename: Gerta
authors.initials: G
authors.lastname: Fleissner
authors.forename: Stefan
authors.initials: S
authors.lastname: Hauptfleisch
authors.forename: Björn
authors.initials: B
authors.lastname: Lemmer
mesh_terms: D000803:Angiotensin I / Q000032:analysis*; D000804:Angiotensin II / Q000032:analysis*; D000818:Animals; D016477:Artifacts; D001369:Axons / Q000648:ultrastructure; D001921:Brain / Q000166:cytology* / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D004727:Endothelium / Q000166:cytology / Q000648:ultrastructure; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D009411:Nerve Endings / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats; D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin / Q000032:analysis / Q000502:physiology*
chemical_list: D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin; D000804:Angiotensin II; D000803:Angiotensin I