[Study of deletion and mutation of p16 gene in primary hepatocellular carcinoma].
OBJECTIVE
To investigate the role the deletion and mutation of p16 gene plays in the pathogenesis of human primary hepatocarcinoma.


METHODS
Thirty-one cases of human hepatocarcinoma, 31 cases of adjacent noncancerous liver cirrhosis and the leukocytes of 8 normal human subjects were analyzed for deletion and mutation in p16 gene exons 1, 2 and introns 1, 2 with comparative multiple PCR and PCR-SSCP.


RESULTS
Deletion of p16 gene exon 1 and partial intron 1 was found in 4 of 31 cases (13/%). No deletion of exon 2 or intron 2 was found. Three patterns of p16 gene intron 1 and 18 bp-flanking sequence in exon 2 at SSCP analysis were observed in hepatocellular carcinoma and corresponding adjacent noncancerous cirrhosis, and two patterns were found in human normal leukocyte DNA. No aberrant single strand at SSCP in p16 gene exon 1 or most part of exon 2 or intron 2 was detected.


CONCLUSIONS
Low frequency of deletion and rare mutation of p16 suppressor gene occurred in hepatocellular carcinoma.
Qin
Y
Y
Laboratory of Molecular Biology, WCUMS, Chengdu 610041, China.
Chen
J
J
Li
B
B
Sun
Z
Z
Zuo
F
F
Sun
Z
Z
D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis* / Q000235:genetics; D005091:Exons; D017353:Gene Deletion; D019942:Genes, p16; D006801:Humans; D007438:Introns; D008113:Liver Neoplasms / Q000235:genetics*; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational
D019941:Cyclin-Dependent Kinase Inhibitor p16