[ATP synthesis by free yeast catalysis coupling hollow fiber ultrafiltration separation].
A novel process for the ATP production employing free yeast catalysis and coupling a hollow fiber module ultrafiltration separation was studied. The experimental results of biocatalyst half-life and operating stability for ultrafiltration showed that the enzymes released from free yeast cell could be cut-off efficiently from the product output stream by hollow fiber membrane. The intercepted percentage of ADH and HK reached more than 95% during the steady running. The efficiency of enzymes utilized was 2.0-2.5 fold as much as that of batch reaction. And the time of yeast cells used repeatedly for ATP synthesis reached 2.5-3.0 batches. Under 0.1 MPa, the ultrafiltrating speed of hollow fiber membrane could keep on a stabilizing value for 11 batches continuously. The continuous operation mode of ATP synthesis simultaneous product ultrafiltration separation could be maintained in higher conversion rate for 5.0 h when the whole system fell in a pseudo-stat where the dilution rate was equal to 0.25 h-1.
Duan
X
X
College of Life Science and Food Engineering, Nanchang University, Nanchang 330047.
Zhang
S
S
Ye
Q
Q
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000426:Alcohol Dehydrogenase / Q000302:isolation & purification; D002384:Catalysis; D006593:Hexokinase / Q000302:isolation & purification; D008567:Membranes, Artificial; D014462:Ultrafiltration; D015003:Yeasts / Q000166:cytology / Q000378:metabolism*
D008567:Membranes, Artificial; D000255:Adenosine Triphosphate; D000426:Alcohol Dehydrogenase; D006593:Hexokinase