[A comparison of the kinetic characters of NT-3, NT-4 and BDNF retrogradely transported in facial nerve].
OBJECTIVE
To compare the kinetic characters of retrograde transport of neurotrophin-3 (NT-3), neurotrophin-4 (NT-4) and brain-derived neurotrophin factor (BDNF) in facial nerve.


METHODS
Radioactive tracer technique was used. After one lateral facial nerve trunk of adult rabbit was transected, a silicone chamber was inserted between the stumps, and 3.7 MBq of 125I-NT-3 or 125I-NT-4 or 125I-BDNF or 125I-HSA was administered into the chamber. At different time-points after injection, the facial nerve trunk and facial nerve motor neurone of brain-stem were collected and the uptake rates were measured. The kinetic parameters of each labeled compound were calculated using 3P87 program of kinetics.


RESULTS
The transport amount of neurotrophin retrogradely transported by facial nerve is NT-3 > BDNF > NT-4 (P < 0.05), the transport rate is NT-4 > NT-3 > BDNF (P < 0.05).


CONCLUSIONS
The findings could serve as the kinetic characters of retrograde transport of neurotrophins.
Li
Y
Y
Department of Nuclear Medicine, First Affiliated Hospital, WCUMS, Chengdu, 610041 China.
Chen
Z
Z
Wang
Q
Q
Liang
Z
Z
Kuang
A
A
He
G
G
Gao
B
B
Lin
D
D
D000818:Animals; D001933:Brain Stem / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000493:pharmacokinetics*; D005154:Facial Nerve / Q000378:metabolism* / Q000601:surgery; D009414:Nerve Growth Factors / Q000493:pharmacokinetics*; D018696:Neuroprotective Agents / Q000493:pharmacokinetics; D020933:Neurotrophin 3 / Q000493:pharmacokinetics*; D011817:Rabbits
D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D020933:Neurotrophin 3; C073922:neurotrophin 4