[Determination of zopiclone in serum by reversed-phase high performance liquid chromatography].
A reversed-phase high performance liquid chromatographic method (external standard method) was developed for the determination of zopiclone in serum. After the selective extraction with n-butyl chloride, this compound was chromatographed on a LiChroCART 125-4 column packed with LiChrospher 60 RP select B(5 microns) using acetonitrile-monopotassium phosphate (20:80, V/V) as mobile phase. The eluting compound was measured by an ultraviolet detector at 254 nm. Spiked with zopiclone of 400 micrograms/L, 1,600 micrograms/L and 6,400 micrograms/L in serum, the recoveries of zopiclone were (73.4 +/- 3.2)%, (82.2 +/- 4.1)% and (90.3 +/- 4.5)% respectively. The detection limit of the method was 15 micrograms/L. The method is simple and rapid for the determination of zopiclone in forensic toxicology.
Yang
Li-jun
LJ
Department of Narcotics Control, Yunnan Public Security College, Kunming 650223, China. ylj666@public.km.yn.cn
Rochholz
Gertrud
G
D000368:Aged; D053961:Azabicyclo Compounds; D002110:Caffeine / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006993:Hypnotics and Sedatives / Q000097:blood* / Q000506:poisoning; D008297:Male; D010879:Piperazines / Q000097:blood* / Q000506:poisoning
D053961:Azabicyclo Compounds; D006993:Hypnotics and Sedatives; D010879:Piperazines; C515050:zopiclone; D002110:Caffeine; C053229:N-desmethylzopiclone