title: Mosaic patch patterns in chimeric and transgenic mice suggest that directional growth in the adrenal cortex begins in the perinatal period.
abstract: Staining for beta-galactosidase (beta-gal) reporter activity in adrenal glands from adult, fetal and neonatal 21-OH/LacZ transgenic mice revealed mosaic patch patterns that were qualitatively similar to those seen in LacZ <--> wild-type mouse chimeras, at similar developmental stages. This suggests that, as in chimeras, the transgenic patch pattern may reflect cell lineage relationships. Consequently, 21-OH/LacZ transgenic mice could be useful as a simpler alternative to chimeras for the analysis of clonal growth and cell mixing during adrenocortical organogenesis. Embryonic day 16.5 (E16.5) adrenal cortices of 21-OH/LacZ transgenic mice displayed a punctate patch pattern, but by E18.5 "stripes" appeared to be emerging and were clearly visible by the day of birth (P0) and three days later (P3), consistent with the adult mosaic striped pattern. This suggests that adrenocortical organogenesis involves an initial period of randomly oriented clonal growth, followed by directional growth which begins in the perinatal period.
authors.affiliation: Clinical Biochemistry Section, Department of Reproductive and Developmental Sciences, University of Edinburgh, UK. steve.morley@ed.ac.uk
authors.forename: Steven D
authors.initials: SD
authors.lastname: Morley
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: O'Donohoe
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Hughes
authors.forename: Catherine
authors.initials: C
authors.lastname: Irving
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Willis
authors.forename: Sarah
authors.initials: S
authors.lastname: Heasman
authors.forename: John D
authors.initials: JD
authors.lastname: West
mesh_terms: D000302:Adrenal Cortex / Q000196:embryology* / Q000254:growth & development*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development*; D005314:Embryonic and Fetal Development; D005260:Female; D007763:Lac Operon; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics; D009030:Mosaicism; D013255:Steroid 21-Hydroxylase / Q000235:genetics
chemical_list: D013255:Steroid 21-Hydroxylase