Structural and functional analysis of 3' untranslated region of mouse Period1 mRNA.
In order to investigate the post-transcriptional regulation of Period1 (Per1), the 3(')-untranslated region (3(')UTR) of mouse Per1 (mPer1) mRNA was characterized. In addition to high similarity between human and mouse Per1 3(')UTRs, AU-rich element and differentiation control element were found in both species. Transient transfection assays using LUC::mPer1 3(')UTR fusion genes revealed that the mPer1 3(')UTR repressed its own expression in a post-transcriptional manner. The region critical for this translational down-regulation was confined to nucleotide positions 322-517. These results suggest that the mPer1 3(')UTR could be involved in the generation of time lag between the transcriptional and translational products of mPer1 in the suprachiasmatic nucleus.
Kojima
Shihoko
S
Laboratory of Functional Genomics, Human Genome Center, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Hirose
Matsumi
M
Tokunaga
Katsushi
K
Sakaki
Yoshiyuki
Y
Tei
Hajime
H
D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D001683:Biological Clocks / Q000502:physiology; D018797:Cell Cycle Proteins; D002940:Circadian Rhythm / Q000502:physiology; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D056950:Period Circadian Proteins; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D013493:Suprachiasmatic Nucleus / Q000502:physiology
D020413:3' Untranslated Regions; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C539022:PER1 protein, human; C539023:Per1 protein, mouse; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins