[Detection of serum type IV collagen and the alteration of this collagen in various liver diseases].
OBJECTIVE
To detect serum Type IV collagen levels in patients with various hepatic diseases.


METHODS
A sandwich enzyme-immunoassay, using two monoclonal antibodies to recognize 7S and TH of Type IV collage, was used to detect serum levels of Type IV collagen in 428 patients with various liver diseases.


RESULTS
Serum levels of Type IV collagen in healthy control subjects were (63.2 +/- 31.9) microgram.L-1. Type IV collagen levels in the serum of patients with heavy hepatitis were the highest [(801.8 +/- 431.2) microgram.L-1]. All Types of hepatic diseases were significantly higher than those in healthy subjects (All P < 0.01).


CONCLUSIONS
The assay used in this study is simple and practical and can be applied to clinical evaluation of hepatic fibrosis.
Wang
J G
JG
Department of Clinical Laboratory, Second Xiangya Hospital, Central South University, Changsha 410011, China.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D024141:Collagen Type IV / Q000097:blood*; D005260:Female; D006506:Hepatitis A / Q000097:blood*; D019694:Hepatitis B, Chronic / Q000097:blood*; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood*; D008297:Male; D008875:Middle Aged
D015415:Biomarkers; D024141:Collagen Type IV