title: [Prokaryote expression and western analysis of BcpLH gene of Chinese cabbage].
abstract: BcpLH gene preferentially expressed in folding leaf of Chinese cabbage contains dsRNA-binding domains. The cDNA of BcpLH gene was cloned into a His-fusion expression vector pET-28a (+) and was induced to express in E. coli strain BL21 (DE3). Then, the specific protein was partially purified and the rabbit was immunized to prepare the anti-serum. Meanwhile BcpLH cDNA was cloned into the pMAL-c2 containing the solubizing partner, and then the soluble protein generated. It was demonstrated from Western dot assay that the BcpLH protein was specific. The BcpLH active protein and its anti-serum made it possible to study RNA-binding activity and regulation mechanism in plant development.
authors.affiliation: Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai 200032.
authors.forename: X H
authors.initials: XH
authors.lastname: Yu
authors.forename: Z R
authors.initials: ZR
authors.lastname: Zheng
authors.forename: P L
authors.initials: PL
authors.lastname: Liu
authors.forename: Y K
authors.initials: YK
authors.lastname: He
mesh_terms: D015153:Blotting, Western; D001937:Brassica / Q000235:genetics*; D015870:Gene Expression; D011387:Prokaryotic Cells / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D016601:RNA-Binding Proteins; D011994:Recombinant Proteins