[Study on production of poly-beta-hydroxybutyrate by recombinant strain VG1(pTU14)].
Cloning and expression of Vitreoscilla hemoglobin gene (vgb) and lambda phage lytic genes (S-RRz) in production of Poly-beta-hydroxybutyrate(PHB) was studied in different Escherichia coli hosts such as E. coliJM105, E. coliJM109 and VG1. In the recombinant strains, VG1(pTU14) was a superior one which simultaneously contained three exogenes including vgb, S-RRz and PHB biosynthesis genes(phbCAB). The experimental results showed that after 82 hours fed-batch cult ure in LBG medium in shaking flask, cell concentration of VG1(pTU14) could reach 25.9 g/L, which was the highest PHB production ever reported, and till 52 h PHB content could be higher than 95%. Additionally, inducible cell lysis was also attained successfully in recombinant VG1(pTU14) accompanying with its high cell density culture. Therefore, VG1(pTU14) is a novel potential strain with promising prospect in industrial production of PHB.
Yu
H
H
Departmnet of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, Beijing 100084.
Yin
J
J
Li
H
H
Yang
S
S
Shen
Z
Z
D001426:Bacterial Proteins / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D015870:Gene Expression; D005814:Genes, Viral; D006454:Hemoglobins / Q000235:genetics*; D006885:Hydroxybutyrates / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism*; D011995:Recombination, Genetic; D054793:Truncated Hemoglobins; D000094122:Polyhydroxybutyrates
D001426:Bacterial Proteins; D006454:Hemoglobins; D006885:Hydroxybutyrates; D011091:Polyesters; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates