title: A closed chamber method for performing biochemical experiments at accurate concentrations of volatile agents.
abstract: Experiments with volatile agents such as general anesthetics present difficulties in maintaining defined concentrations of these agents during in-vitro experimental conditions. In conventional filtration apparatuses, due to their partition between liquid and vapor phases (to open air or headspaces of the incubation vehicles), some degree of inaccuracy in calculated concentrations of these agents may occur in experiments using these types of chambers. In the present study, a method is described which permits the performance of biochemical experiments in a closed system in which the concentrations of a volatile agent, desflurane, in the liquid phase of the assay environment can be maintained constant for a relatively long time period.
authors.affiliation: National Institute on Drug Abuse/IRP, Cellular Neurobiology Section, NationalInstitutes of Health, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA. moz@intra.nida.nih.gov
authors.forename: Murat
authors.initials: M
authors.lastname: Oz
authors.forename: Alp
authors.initials: A
authors.lastname: Alptekin
authors.forename: Meral
authors.initials: M
authors.lastname: Dinc
mesh_terms: D018685:Anesthetics, Inhalation / Q000737:chemistry; D001671:Biochemistry / Q000379:methods; D001703:Biophysics / Q000379:methods*; D002118:Calcium / Q000378:metabolism; D000077335:Desflurane; D004305:Dose-Response Relationship, Drug; D007530:Isoflurane / Q000031:analogs & derivatives* / Q000737:chemistry*; D013997:Time Factors
chemical_list: D018685:Anesthetics, Inhalation; D000077335:Desflurane; D007530:Isoflurane; D002118:Calcium