title: [Expression of recombinant human serum albumin in genetically engineered Pichia pastoris in high-density fermentation].
abstract: The optimum culture conditions of genetically engineered Pichia pastoris in shake-flask cultivation and in fed-batch fermentation were investigated respectively in this paper. It showed that the cultural period induced with 5 g/L methanol is 96 h, optimum methanol concentration is 10 g/L, and pH range is 5.72-6.59 in shake-flask culture. Although the seed inoculum amount increased, the target protein productivity per cell optical density was decreased. Their relationship fit the equeation Y = 12.941x(-0.5059) (r = 0.9789, where x is inoculating OD600, Y is protein productivity per cell optical density), we postulated that the restricting factor may be dissolved oxygen (DO) at shake-flask culture. With the 10% inoculum and 20 OD600 of seed, the lag phase of cell growth is 2.11 h in batch cultivation, and the relationship between cell optical density (Y) and culture time (t) is Y = 0.7841e0.2319t (r = 0.9936); The cell dry weight of broth reached 115-160 g/L and the maximum rHSA concentration was 3.6 g/L at the 120th at the fed-batch fermentation phase.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, China.
authors.forename: Meijin
authors.initials: M
authors.lastname: Guo
authors.forename: Yingping
authors.initials: Y
authors.lastname: Zhuang
authors.forename: Ju
authors.initials: J
authors.lastname: Chu
authors.forename: Siliang
authors.initials: S
authors.lastname: Zhang
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Liu
mesh_terms: D005285:Fermentation; D005818:Genetic Engineering; D010843:Pichia / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis; D012709:Serum Albumin / Q000096:biosynthesis*
chemical_list: D011994:Recombinant Proteins; D012709:Serum Albumin