title: Independent subsets of amplified fragments from the genome of Neisseria meningitidis identify the same invasive clones of ET37 and ET5.
abstract: To determine whether fluorescent amplified-fragment length polymorphism (FAFLP) analysis is an unbiased genome sampling technique, data were analysed from three different primer combinations, amplifying three independent fragment subsets from 123 isolates of Neisseria meningitidis. Using these data, dendrograms were generated with near-identical topologies that identified the same invasive clones of ET37 and ET5 and also identified the same outbreak clusters.
authors.affiliation: Department of Academic Paediatrics, Imperial College at St Mary's Hospital, London, UK 2Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK 3,4Molecular Bacteriology and Immunology Group, Division of Microbiology3 and Department of Public Health Medicine and Epidemiology4, University of Nottingham, Queen's Medical Centre, Nottingham, UK.
authors.forename: Jonathan N
authors.initials: JN
authors.lastname: Goulding
authors.affiliation: Department of Academic Paediatrics, Imperial College at St Mary's Hospital, London, UK 2Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK 3,4Molecular Bacteriology and Immunology Group, Division of Microbiology3 and Department of Public Health Medicine and Epidemiology4, University of Nottingham, Queen's Medical Centre, Nottingham, UK.
authors.forename: John
authors.initials: J
authors.lastname: Stanley
authors.affiliation: Department of Academic Paediatrics, Imperial College at St Mary's Hospital, London, UK 2Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK 3,4Molecular Bacteriology and Immunology Group, Division of Microbiology3 and Department of Public Health Medicine and Epidemiology4, University of Nottingham, Queen's Medical Centre, Nottingham, UK.
authors.forename: Will
authors.initials: W
authors.lastname: Olver
authors.affiliation: Department of Academic Paediatrics, Imperial College at St Mary's Hospital, London, UK 2Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK 3,4Molecular Bacteriology and Immunology Group, Division of Microbiology3 and Department of Public Health Medicine and Epidemiology4, University of Nottingham, Queen's Medical Centre, Nottingham, UK.
authors.forename: Keith R
authors.initials: KR
authors.lastname: Neal
authors.affiliation: Department of Academic Paediatrics, Imperial College at St Mary's Hospital, London, UK 2Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK 3,4Molecular Bacteriology and Immunology Group, Division of Microbiology3 and Department of Public Health Medicine and Epidemiology4, University of Nottingham, Queen's Medical Centre, Nottingham, UK.
authors.forename: Dlawer A A
authors.initials: DAA
authors.lastname: Ala'Aldeen
authors.affiliation: Department of Academic Paediatrics, Imperial College at St Mary's Hospital, London, UK 2Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK 3,4Molecular Bacteriology and Immunology Group, Division of Microbiology3 and Department of Public Health Medicine and Epidemiology4, University of Nottingham, Queen's Medical Centre, Nottingham, UK.
authors.forename: Catherine
authors.initials: C
authors.lastname: Arnold
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004196:Disease Outbreaks; D005456:Fluorescent Dyes; D005784:Gene Amplification; D016680:Genome, Bacterial; D006801:Humans; D008589:Meningococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D009345:Neisseria meningitidis / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D006113:United Kingdom / Q000453:epidemiology
chemical_list: D004269:DNA, Bacterial; D005456:Fluorescent Dyes