Glycoproteins of chicken liver nuclei cross-linked to DNA by cis-diamminedichloroplatinum.
Glycoproteins which participate in DNA-protein cross-links induced by action of cis-diamminedichloroplatinum (cis-DDP) in intact nuclei of chicken liver were investigated. Digoxigenin-labelled lectins with different sugar specificity were used for detection and characterization of these glycoproteins. Our results showed the presence of glycoproteins bearing high mannose as well as complex type oligosaccharides in chicken liver nuclei. In most cases of complex oligosaccharides, sialic acid residues bound in alpha(2-6) but not in alpha(2-3) linkage were present.
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Department of Cytobiochemistry, University of Lódź, Lódź, Poland.
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D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D002645:Chickens; D002945:Cisplatin / Q000494:pharmacology; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000187:drug effects; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D046911:Macromolecular Substances; D009687:Nuclear Proteins / Q000378:metabolism; D011485:Protein Binding
D004268:DNA-Binding Proteins; D006023:Glycoproteins; D037102:Lectins; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D004247:DNA; D002945:Cisplatin