Lipopolysaccharides induce intestinal serum amyloid A expression in the sea cucumber Holothuria glaberrima.
We have previously characterized the first invertebrate homolog of serum amyloid A (SAA) proteins in the sea cucumber Holothuria glaberrima, where its expression is associated with intestinal regeneration, suggesting a possible involvement of SAA proteins in intestinal morphogenesis. Here we show that bacterial lipopolysaccharides (LPS) trigger a coelomocyte-mediated immune response in H. glaberrima, inducing an approximately threefold increase in coelomocyte phagocytic activity. Furthermore, LPS induces an approximately fourfold increase in SAA mRNA levels in non-regenerating intestines. These results show that in H. glaberrima, LPS act as an immune activator and that SAA expression can be modulated by immune-associated processes.
Santiago-Cardona
Pedro G
PG
Department of Biology, University of Puerto Rico, UPR Station, PR 00931-3360, Río Piedras, Puerto Rico.
Berríos
César A
CA
Ramírez
Francisco
F
García-Arrarás
José E
JE
D000818:Animals; D007413:Intestinal Mucosa / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D010587:Phagocytosis; D012333:RNA, Messenger / Q000032:analysis; D012038:Regeneration; D012616:Sea Cucumbers / Q000276:immunology*; D000685:Serum Amyloid A Protein / Q000235:genetics*
D008070:Lipopolysaccharides; D012333:RNA, Messenger; D000685:Serum Amyloid A Protein