title: Nitric oxide and its target cells in cercaria of Diplostomum chromatophorum: a histochemical and immunocytochemical study.
abstract: In order to advance our knowledge of the nitrergic nervous system in flatworms, the patterns of the NADPH-diaphorase (NADPH-d) reaction and cGMP immunoreactivity, after stimulation with a nitric oxide donor in the presence of an inhibitor of phosphodiesterase, were investigated in cercaria of Diplostomum chromatophorum. This is the first time the presence of NADPH-d activity has been detected in a larval fluke, and the first time the presence of cGMP immunoreactivity has been detected in a flatworm. The NADPH-d reaction occurs in the ventral sucker, the hind body and the tail. cGMP immunoreactivity was detected in the muscle cells of the body and in two pairs of sensory cells at the anterior end of the body and in the middle of the furca. The sensory cells also showed 5-HT immunoreactivity. The spatial relationship between the cGMP and the 5-HT immunoreactivities was studied. By staining with TRITC-labelled phalloidin, the pattern of the muscle fibres was revealed.
authors.affiliation: Institute of Parasitology of the Russian Academy of Sciences, Lenin Avenue 33, 117071 Moscow, Russia.
authors.forename: Nadezhda B
authors.initials: NB
authors.lastname: Terenina
authors.forename: Margaretha K S
authors.initials: MK
authors.lastname: Gustafsson
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D006152:Cyclic GMP / Q000032:analysis / Q000276:immunology; D006651:Histocytochemistry; D007150:Immunohistochemistry; D008018:Life Cycle Stages; D009252:NADPH Dehydrogenase / Q000032:analysis / Q000276:immunology; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D010590:Phalloidine / Q000378:metabolism; D012235:Rhodamines / Q000737:chemistry; D012701:Serotonin / Q000737:chemistry; D013194:Staining and Labeling; D014200:Trematoda / Q000737:chemistry / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism
chemical_list: D000199:Actins; D020030:Nitric Oxide Donors; D012235:Rhodamines; D010590:Phalloidine; D009569:Nitric Oxide; D012701:Serotonin; C009434:tetramethylrhodamine isothiocyanate; D009252:NADPH Dehydrogenase; D006152:Cyclic GMP