title: Analysis of oropouche virus L protein amino acid sequence showed the presence of an additional conserved region that could harbour an important role for the polymerase activity.
abstract: We described here the complete nucleotide sequence of the L RNA segment of Oropouche virus (genus Orthobunyavirus, family Bunyaviridae). We found the L RNA segment is 6846 nucleotides long and encodes a putative RNA polymerase of 2250 amino acids. Phylogenetic analysis showed that ORO virus cluster to the Orthobunyavirus genus confirming the serological classification. It also showed that Bunyamwera and California viruses, from the Orthobunyavirus genus, are more closely related to each other than to ORO virus. Sequence comparisons performed between the L proteins of 15 bunyaviruses and the PB1 proteins of 3 influenza viruses revealed that ORO L protein contains the 3 regions characteristic of arenaviruses and bunyaviruses. These comparisons also showed the existence of an additional fourth conserved region in the L protein of bunyaviruses that contains at least two active sites.
authors.affiliation: Unidade Multidepartamental de Pesquisa em Virologia, Faculdade de Medicina de Ribeirão Preto, USP, Ribeirão Preto, Brasil.
authors.forename: V H
authors.initials: VH
authors.lastname: Aquino
authors.forename: M L
authors.initials: ML
authors.lastname: Moreli
authors.forename: L T
authors.initials: LT
authors.lastname: Moraes Figueiredo
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism*; D002141:Encephalitis Virus, California / Q000737:chemistry / Q000235:genetics; D016679:Genome, Viral; D018062:La Crosse virus / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009975:Orthomyxoviridae / Q000737:chemistry / Q000235:genetics; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics
chemical_list: C069882:L protein, Bunyamwera virus; D012367:RNA, Viral; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases