[Studies on the methods of mating test for Cryptococcus neoformans].
Six media (MA:Mating agar, HA:Hay agar, HCA:Hay cube agar, RSA:Rice shell agar, IHA:additional nutrition in HA and SDA:Sabouraudia dextrose agar) were compared for their efficiencies of the mating test for Cryptococcus neoformans. The positive rates in 2-week-cultivation were 95% (HCA and RSA), 86% (HA), 38% (MA and SDA) and 24% (IHA). The streaking inoculation was equally replaced by the dot inoculation for the mating tests. However, the dot method saves much more materials, and is suitable for testing in large number of isolates. Additionally, the precultivation of the tester and tested isolates in broth medium (YMPDB) could enhance the positive mating rates.
Li
A
A
Institute of Dermatology, Chinese Academy of Medical Science, Nanjing 210042, China.
Lu
G
G
Chen
W
W
Shen
Y
Y
Wu
S
S
D000362:Agar; D003455:Cryptococcus neoformans / Q000254:growth & development* / Q000502:physiology*; D003470:Culture Media; D010641:Phenotype; D012098:Reproduction / Q000502:physiology*
D003470:Culture Media; D000362:Agar