title: [Studies on the active constituents of sichuan liang shan rhododendron L. I. Determination of two flavonoids in leaves, extracts and jinjuan liquors].
abstract: A reversed-phase HPLC method for the separation and determination of the two flavonoids: quercetin and kaempferol is described. The column was 25 cm x 4.6 mm i.d., packed with Zorbax SB-C18(5 microns). The mobile phase was a mixture of MeOH-H2O (60:40, V/V). The flow rate was 0.7 mL/min. UV detection was performed at 360 nm. External standard was used and the calibration curves showed good linearity over the range of 6.0-100 mg/L, r = 0.9995-0.9998, and detection limits were 3.3-6.2 mg/L. The recoveries were 96.0% and 97.3% for quercetin and kaempferol respectively. The relative standard deviations of the method were 2.1%-4.5%. The method is simple, fast, sensitive and accurate and can be applied to the analysis of quercetin and kaempferol. It provides a scientific basis for industrial production and quality control of Jinjuan liquor preparations for clinical uses.
authors.affiliation: Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: H X
authors.initials: HX
authors.lastname: Li
authors.forename: M Y
authors.initials: MY
authors.lastname: Ding
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: P R
authors.initials: PR
authors.lastname: Chen
authors.forename: W Q
authors.initials: WQ
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000302:isolation & purification; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification; D044949:Kaempferols; D018515:Plant Leaves / Q000737:chemistry; D011794:Quercetin / Q000032:analysis* / Q000302:isolation & purification; D029793:Rhododendron / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D044949:Kaempferols; C006552:kaempferol; D011794:Quercetin