[Determination of phospholipid in the peroxisomal membrane of rat liver by high performance liquid chromatography].
A method was employed for the determination of phospholipid in membrane of peroxisomes by high performance liquid chromatography. The separation was performed on a mu-Porasil Si60 column(250 mm x 4.6 mm i.d.) by gradient elution. The phospholipids were detected at 205 nm. The limits of detection for cardiolipin, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine and sphingomyelin were 12, 8, 23, 4, 23 and 7 ng respectively and the recoveries were between 84% and 103%. The linear relationship of calibration curves was good (r > 0.91). The phospholipids in peroxisomal membrane of 10 SD rat liver were determined and the results are satisfactory.
Jiang
Q F
QF
Institute of Basic Medicine, Medical University of Hebei, Shijiazhuang 050017, China.
Luo
Z S
ZS
Li
X F
XF
Jiang
L L
LL
D000818:Animals; D002308:Cardiolipins / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000737:chemistry*; D020675:Peroxisomes / Q000737:chemistry*; D010716:Phosphatidylinositols / Q000032:analysis; D010718:Phosphatidylserines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D002308:Cardiolipins; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; D010743:Phospholipids