Potentiation of alpha7-containing nicotinic acetylcholine receptors by select albumins.
Nicotinic receptors containing alpha7 subunits are ligand-gated ion channels widely distributed in the nervous system; they influence a diverse array of events because of their high relative calcium permeability. We show here that nicotine-induced whole-cell responses generated by such receptors can be dramatically potentiated in a rapidly reversible manner by some but not all albumins. The potentiation involves increases both in potency and efficacy with no obvious differences in rise and fall times of the response. The potentiation is not reduced by removing absorbed components; it is abolished by proteolysis, suggesting that the albumin protein backbone is essential. The fact that some albumins are ineffective indicates that minor differences in amino acid sequence may be critical. Experiments with open channel blockers indicate that the potentiation involves increased responses from active receptors rather than recruitment of receptors from a previously silent pool. Single channel recordings reveal that the potentiation correlates with increased single channel opening probability, reflected in increased frequency of channel opening and increased mean channel open time. The potentiation can be exploited to overcome blockade by noncompetitive inhibitors such as beta-amyloid peptide. The results raise the possibility that endogenous compounds use the site to modulate receptor function in vivo, and suggest that the receptors may represent useful targets for therapeutic intervention in cases where they have been implicated in neuropathologies such as Alzheimer's disease.
Conroy
William G
WG
Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093, USA.
Liu
Qing-Song
QS
Nai
Qiang
Q
Margiotta
Joseph F
JF
Berg
Darwin K
DK
D000418:Albumins / Q000737:chemistry / Q000494:pharmacology*; D016229:Amyloid beta-Peptides / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D004594:Electrophysiology; D010446:Peptide Fragments / Q000494:pharmacology; D021122:Protein Subunits / Q000187:drug effects*; D011978:Receptors, Nicotinic / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D000418:Albumins; D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D021122:Protein Subunits; D011978:Receptors, Nicotinic; C078592:amyloid beta-protein (1-40); D012710:Serum Albumin, Bovine