[Preparation, purification and identification of sialic acid from Escherichia coli C-8].
Colominic acid produced from Escherichia coli C-8 was purified by ammonia sulfate precipitation, dialysis and concentration. After hydrolysis of colominic acid at a pH of 2.5, at 70 degrees C for 4 h, sialic acid was obtained, then purified by chromatography on Dowex1-x8. Analysis of thin-layer chromatography and absorption spectrum of sialic acid in the orcinol/Fe3+/HCl and the periodic acid/thiobarbituric acid confirmed that its purity was identical with that of standard sample.
Qian
S
S
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
Li
J
J
Gao
J
J
Guo
L
L
Ye
J
J
D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D012794:Sialic Acids / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D013057:Spectrum Analysis
D012794:Sialic Acids