[The construction and application of promoter-signal peptide probe vector of lactic acid bacteria].
A promoter-signal peptide probe vector of lactic acid bacteria, designated pZLB based on pMG36e has been constructed. The vector contained a reporterd gene encoding for beta-lactamase, which lacked a promoter and a functional signal sequence. Sau3A restriction fragments (80-400 bp) from L. lactis ATCC 7962 total DNA were cloned into BamHI site of pZLB. A lot of different size framents were selected. These fragments promoted transcription and secretion of beta-lactamase in L. lactis. Sequence analysis revealed that inserted fragment in pZLBe7 contained typical promoter, SD sequence and atypical signal sequence.
Chen
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X
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Liu
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Z
Huan
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D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D017930:Genes, Reporter; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D021382:Protein Sorting Signals; D001618:beta-Lactamases