title: Development of the single-cell MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) mass-spectroscopic assay.
abstract: MALDI-TOF mass spectrometry was used to detect intracellular molecules from a single intact cell on monolayers of other cells. Intracellular molecules, e.g., histamine, were gradually increased in a mouse bone marrow-derived mast cell by a maturation process. A single cell was captured by a microsuction pipette, and the mass spectra of intracellular histamine were measured directly. Finally, the time course of the intracellular molecular contents and the maturation stage from a single cell were estimated.
authors.affiliation: Department of Analytical Life Science, Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Shimizu
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Ojima
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ohnishi
authors.forename: Tomoteru
authors.initials: T
authors.lastname: Shingaki
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Hirakawa
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Masujima
mesh_terms: D016475:3T3 Cells; D000818:Animals; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D005347:Fibroblasts / Q000166:cytology; D008407:Mast Cells / Q000166:cytology; D051379:Mice; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D017077:Culture Media, Conditioned