[Studies on the trehalose-producing by Colloides CB39].
A bacterium which had the ability of producing sugar produced sugar at low temperature (18 degrees C) was selected from the water of Tianchi in Changbai mountain. The sugar was identified to be trehalose by methods of thin-layer chromatography, reaction of producing osazyone, capillary electrophoresis and infrared spectrum. It was also been found that the trehalose produced by this bacterium (identified to be a new species of Colloides Sp. CB39) was exocrine. At 18 degrees C, its trehalose content in culture solution was 256 mg/g dry weight of the strain. This characteristic is different from that of other strains, which had been proved to have the ability of producing trehalose. then the strain CB39 was induced by ultraviolet in order to abstain mutants which can produce trehalose in high level at usual temperature. The mutant strain 5 that could produce trehalose in high level at usual temperature (25 degrees C) was selected from all the mutant strains. The value of its productivity of trehalose is 416.7 mg/g dry weight of the strain. (At the same temperature, trehalose productivity of mutant strain 5 was eight times of that of the wild type strain CB39).
Zheng
Y
Y
State Key Laboratory of Enzyme Engineering, Jilin University, Changchun 130023.
Zhang
H
H
Zhao
Y
Y
Zhang
J
J
D001419:Bacteria / Q000145:classification* / Q000378:metabolism* / Q000528:radiation effects; D005618:Fresh Water / Q000382:microbiology; D016296:Mutagenesis; D014199:Trehalose / Q000378:metabolism*; D014466:Ultraviolet Rays
D014199:Trehalose