title: [Activation of G protein on the membrane of TCS-sensitive cells].
abstract: Trichosanthin (TCS), a Type I Ribosome Inactivating Protein isolated from the root tuber of Trichosanthes Kirilowii M. has various biological activities including abortion induction, anti-tumor and anti-HIV. The mechanism of TCS specifically killing sensitive cells has not been studied clearly. In this study, we initially found that there exists TCS-affinity molecule on Syncytiotrophoblast cells and Jar cells. Furthermore, by [35S]GTP gamma S Binding Assay, we find that TCS can activate G protein on the membrane of TCS-sensitive cells. These results indicate that on the membrane of TCS-sensitive cells exists TCS-specific receptor.
authors.affiliation: Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Wu
authors.forename: H L
authors.initials: HL
authors.lastname: Nie
authors.forename: B
authors.initials: B
authors.lastname: Lu
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: Y B
authors.initials: YB
authors.lastname: Ke
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D019204:GTP-Binding Proteins / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D020014:K562 Cells; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015978:Trichosanthin / Q000494:pharmacology*; D014327:Trophoblasts / Q000166:cytology
chemical_list: D011956:Receptors, Cell Surface; D015978:Trichosanthin; D019204:GTP-Binding Proteins