Cartilage regeneration using slow release of bone morphogenetic protein-2 from a gelatin sponge to treat experimental canine tracheomalacia: a preliminary report.
We investigated whether saber sheath-type tracheomalacia could be treated by the slow release of bone morphogenetic protein (BMP)-2 from a gelatin sponge. A 1 cm gap was made in the middle portion of each of 10 consecutive tracheal cartilage rings in the canine cervix (control group, n = 3), then a gelatin sponge containing 12 microg of BMP-2 solution was implanted in the gap (12 microg group, n = 3). In another group (120 microg + P group, n = 3), the implanted gelatin sponge contained 120 microg of BMP-2 solution, and the gap was covered with periosteum. All of the control dogs developed saber sheath-type tracheomalacia, whereas tracheomalacia was not observed in the 12 microg and 120 microg + P groups. In the 12 microg group, fibrous cartilage was observed at the ends of the cartilage stumps. In the 120 microg + P group, newly formed bone and cartilage were observed to form a bridge between the cartilage stumps. The regeneration of cartilage or bone induced by the slow release of BMP-2 from a gelatin sponge might be useful for treatment of tracheomalacia.
Okamoto
Taku
T
Second Department of Surgery, Kagawa Medical University, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa, Japan 761-0793.
Yamamoto
Yasumichi
Y
Gotoh
Masashi
M
Liu
Dage
D
Kihara
Minoru
M
Kameyama
Kotaro
K
Hayashi
Eiichi
E
Nakamura
Kenji
K
Yamauchi
Akira
A
Huang
Cheng-Long
CL
Yokomise
Hiroyasu
H
Yamamoto
Masaya
M
Nakamura
Tatsuo
T
Shimizu
Yasuhiko
Y
Tabata
Yasuhiko
Y
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D001999:Bronchoscopy; D002356:Cartilage / Q000502:physiology*; D002357:Cartilage Diseases / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D004285:Dogs; D005780:Gelatin; D012038:Regeneration / Q000187:drug effects*; D015503:Surgical Sponges; D014133:Tracheal Diseases / Q000188:drug therapy* / Q000473:pathology; D016212:Transforming Growth Factor beta
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D016212:Transforming Growth Factor beta; D005780:Gelatin