title: Immune gene discovery by expressed sequence tags generated from hemocytes of the bacteria-challenged oyster, Crassostrea gigas.
abstract: An expressed sequence tag program was undertaken to isolate genes involved in defense mechanisms of the Pacific oyster, Crassostrea gigas. Putative function could be assigned to 54% of the 1142 sequenced cDNAs. We built a public database where all EST information are accessible through numerous search profiles (http://www.ifremer.fr/GigasBase). Based on sequence similarities we identified 20 genes that may be implicated in immune function. We investigated the expression of four of these genes during bacterial challenge of oysters. Three of them were induced in response to challenge lending support to their involvement in oyster immunity. Moreover, four other genes were highly homologous to components of the NF-kappa B signaling pathway which is involved in innate immune response in Drosophila and mammals. Altogether, our results open a new way to investigate the immune response in mollusks.
authors.affiliation: Défense et Résistance chez les Invertébrés Marins, UMR5098 IFREMER-CNRS-UMII, Université de Montpellier II, 2 place Eugène Bataillon, CC80, France.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Gueguen
authors.forename: Jean Paul
authors.initials: JP
authors.lastname: Cadoret
authors.forename: Didier
authors.initials: D
authors.lastname: Flament
authors.forename: Catherine
authors.initials: C
authors.lastname: Barreau-Roumiguière
authors.forename: Anne Laure
authors.initials: AL
authors.lastname: Girardot
authors.forename: Julien
authors.initials: J
authors.lastname: Garnier
authors.forename: Alixe
authors.initials: A
authors.lastname: Hoareau
authors.forename: Evelyne
authors.initials: E
authors.lastname: Bachère
authors.forename: Jean Michel
authors.initials: JM
authors.lastname: Escoubas
mesh_terms: D000818:Animals; D001419:Bacteria / Q000276:immunology; D001773:Blood Cells / Q000166:cytology; D015815:Cell Adhesion Molecules / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics; D020224:Expressed Sequence Tags; D005293:Ferritins / Q000235:genetics; D005786:Gene Expression Regulation / Q000276:immunology; D006434:Hemocytes / Q000378:metabolism*; D007113:Immunity, Innate / Q000235:genetics*; D008668:Metallothionein / Q000235:genetics; D010125:Ostreidae / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics
chemical_list: D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D019714:Tissue Inhibitor of Metalloproteinases; D005293:Ferritins; D008668:Metallothionein; D010450:Endopeptidases