title: 3'-Ferrocene-labeled oligonucleotide chains end-tethered to gold electrode surfaces: novel model systems for exploring flexibility of short DNA using cyclic voltammetry.
abstract: The flexibility of DNA is of central importance in biology, medicine, materials science, and mechanical engineering. In this study, we report an unprecedented electrochemical approach for investigating the flexibility of a short (typically 20-base), surface end-tethered single-stranded synthetic DNA oligonucleotide and of its postformed DNA duplex, taking as an example the homopolymer (dT)20 sequence in the regime of very high ionic strength ( approximately 1 M).
authors.affiliation: Laboratoire d'Electrochimie Moléculaire, Unité Mixte de Recherche Université-CNRS No 7591, Université de Paris 7 - Denis Diderot, 2 place Jussieu, 75251 Paris Cedex 05, France. anne@paris7.jussieu.fr
authors.forename: Agnès
authors.initials: A
authors.lastname: Anne
authors.forename: Agnès
authors.initials: A
authors.lastname: Bouchardon
authors.forename: Jacques
authors.initials: J
authors.lastname: Moiroux
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000031:analogs & derivatives; D018076:DNA, Complementary / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D000075163:Metallocenes; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*
chemical_list: D018076:DNA, Complementary; D004277:DNA, Single-Stranded; D004220:Disulfides; D005296:Ferrous Compounds; D000075163:Metallocenes; D009841:Oligonucleotides; D006046:Gold; D003545:Cysteine; C004998:ferrocene