[Production and some properties of a thermophilic protease from Bacillus stearothermophilus WF146].
The factors affecting Bacillus stearothermophilus WF146 for thermophilic protease producing have been investigated, more than 600 units of enzyme in 1 mL of fermented culture could be achieved under suitable condition. The protease had a molecular weight around 34 kD estimated by SDS-PAGE, and functioned optimally at pH 8.0 and 80 degrees C, respectively. In addition, the enzyme exhibited high temperature tolerance and was stable at a wide range of pH, and Ca2+ played a key role for the stability of the enzyme. While the protease activity of the enzyme was strongly inhibited by PMSF, DFP and IAA, and was not affected by DTT.
Tang
B
B
College of Life Sciences, Wuhan University, Wuhan 430072.
Zhou
L
L
Chen
X
X
Dai
X
X
Peng
Z
Z
D010450:Endopeptidases / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D004795:Enzyme Stability; D005285:Fermentation; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D013696:Temperature
D010450:Endopeptidases