[The determination of lorazepam in human urine by gas chromatography/nitrogen-phosphorus detector].
A method to assay lorazepam in human urine has been developed. After addition of hydroxyethylflurazepam (internal standard) and hydrolysis with beta-glucuronidase, the lorazepam and hydroxyethylflurazepam were extracted with ethyl ether at pH 10.8. The analysis was performed on an HP-5 capillary column with nitrogen-phosphorus detector(NPD). The detection limit and recovery of analytes in urine were 5 micrograms/L and (83.4 +/- 3.1)% respectively. The method was successfully applied to urine specimens collected from healthy human volunteers who have ingested 2 mg of lorazepam. The method was sensitive enough to assay urine specimen excreted at 32 h after taking the medicine by volunteers.
Jiang
Z L
ZL
Dept. of Forensic Medicine, China Criminal Police College, Shenyang 110035, China.
Tan
J Y
JY
Yao
L J
LJ
Xing
L M
LM
D014151:Anti-Anxiety Agents / Q000652:urine*; D002849:Chromatography, Gas / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008140:Lorazepam / Q000652:urine*; D008297:Male; D008875:Middle Aged; D009584:Nitrogen / Q000032:analysis; D010758:Phosphorus / Q000032:analysis
D014151:Anti-Anxiety Agents; D010758:Phosphorus; D009584:Nitrogen; D008140:Lorazepam