[Cloning of DNA sequences involved in exopolysaccharide synthesis of Xanthomonas campestris pv. campestris].
By using a cloned DNA segment containing the mutated sequences from the exopolysaccharide-deficient mutant T117 of Xanthomonas campestris pv. campestris as a probe, a 9.4 kb HindIII fragment harbouring DNA sequences corresponding to the mutated site was identified and cloned from the wild-type strain. The fragment could complement the mutant strain T117 in trans and restore the synthesis of exopolysaccharide. This indicates that the fragment contains at least an intact gene which is involved in the exopolysaccharide synthesis of Xanthomonas campestris pv. campestris.
Zha
D
D
Laboratory of Molecular Genetics, Guangxi Agricultural University, Nanning 530005.
Tang
J
J
Ma
Q
Q
D003001:Cloning, Molecular; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D014974:Xanthomonas / Q000235:genetics*
D015342:DNA Probes; D004269:DNA, Bacterial; D011135:Polysaccharides, Bacterial