[Determination of organic acids in fermentation broth of spiramycin by high performance liquid chromatography].
A method for determining organic acids in spiramycin fermentation broth by high performance liquid chromatography is described. The operating conditions were Zorbax 300-SB C18 column (5 microns, 4.6 mm i.d. x 15 cm) at 35 degrees C, 0.01 mol/L phosphoric acid buffer solution (pH 2.32) and methanol as mobile phase at a flow rate of 0.6 mL/min and UV detection at 210 nm. The relative standard deviations were 0.33%-0.10% and the recoveries were 99.95%-100.08%. It's a simple, rapid and accurate method.
Li
You-yuan
YY
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Chen
Chang-hua
CH
Tao
Ping
P
D019342:Acetic Acid / Q000032:analysis*; D020148:Butyric Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005285:Fermentation; D011422:Propionates / Q000032:analysis; D015572:Spiramycin / Q000032:analysis*
D011422:Propionates; D020148:Butyric Acid; D015572:Spiramycin; C029658:propionic acid; D019342:Acetic Acid