title: Bone regeneration following injection of mesenchymal stem cells and fibrin glue with a biodegradable scaffold.
abstract: OBJECTIVE
  The purpose of this study was to determine whether a combination of fibrin glue, beta-tricalcium phosphate as a biodegradable (beta-TCP) and mesenchymal stem cells would provide three-dimensional templates for bone growth resulting in new bone formation at heterotopic sites in the rat with plasticity.
  
  
  METHODS
  Growing stem cells and developing matrices, explanted from the rat femur, were fragmented and mixed with fibrin glue in a syringe. The cells/beta-TCP fibrin glue admixtures were injected into the subcutaneous space on the dorsum of the rat.
  
  
  RESULTS
  Eight weeks after implantation, gross morphology revealed a pearly opalescence and firm consistency. Histological inspections showed newly formed bone structures in all admixtures, but none in the control groups when only fibrin glue and beta-TCP were injected. Osteopontin, a protein important in bone development, was identified by using antibodies in all cells/beta-TCP fibrin glue admixtures.
  
  
  CONCLUSIONS
  Mesenchymal stem cells/beta-TCP fibrin glue admixtures can result in successful bone formation. This technique holds the promise of a minimally invasive means of generating autogenous bone to correct or reconstruct bony defects.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Nagoya University, Graduate School of Medicine, Japan
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Yamada
authors.forename: Jae Seong
authors.initials: JS
authors.lastname: Boo
authors.forename: Ryotaro
authors.initials: R
authors.lastname: Ozawa
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Nagasaka
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Okazaki
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Hata
authors.forename: Minoru
authors.initials: M
authors.lastname: Ueda
mesh_terms: D000818:Animals; D000906:Antibodies; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000627:therapeutic use*; D002130:Calcium Phosphates / Q000627:therapeutic use*; D002516:Ceramics / Q000627:therapeutic use*; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D008297:Male; D016555:Matched-Pair Analysis; D008648:Mesoderm / Q000166:cytology / Q000502:physiology*; D009999:Ossification, Heterotopic / Q000378:metabolism / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D053495:Osteopontin; D010750:Phosphoproteins / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D012795:Sialoglycoproteins / Q000032:analysis; D013234:Stem Cells / Q000502:physiology*; D014014:Tissue Adhesives / Q000627:therapeutic use*
chemical_list: D000906:Antibodies; D018786:Bone Substitutes; D002130:Calcium Phosphates; D015718:Fibrin Tissue Adhesive; D010750:Phosphoproteins; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D014014:Tissue Adhesives; C485817:beta-tricalcium phosphate; D053495:Osteopontin; C018392:tricalcium phosphate