title: Genome-wide analyses on loss of heterozygosity in head and neck squamous cell carcinomas.
abstract: Head and neck squamous cell carcinoma (HNSCC) is a frequent malignancy with a poor survival rate. Identifying the tumor suppressor gene (TSG) loci by genomic studies is an important step to uncover the molecular mechanisms involved in HNSCC pathogenesis. We therefore performed comprehensive analyses on loss of heterozygosity (LOH) using a genome-wide panel of 191 microsatellite markers in 22 HNSCC samples. We found 53 markers with significantly high LOH (>30%) on 21 chromosomal arms; the highest values of those were observed on 3p, 9p, 13q, 15q, and 17p, corresponding to D3S2432 (67%), D9S921-D9S925 (67%) and GATA62F03 (86%), D13S1493 (60%), D15S211 (62%), and D17S1353 (88%), respectively. Fifteen hot spots of LOH were defined in 13 chromosomal arms: 2q22-23, 4p15.2, 4q24-25, 5q31, 8p23, 9p23-24, 9q31.3, 9q34.2, 10q21, 11q21-22.3, 14q11-13, 14q22.3, 17p13, 18q11, and 19q12 as loci reported previously in HNSCCs. Furthermore, we identified five novel hot spots of LOH on three chromosomal arms in HNSCC at 2q33 (D2S1384), 2q37 (D2S125), 8q12-13 (D8S1136), 8q24 (D8S1128), and 15q21 (D15S211). In conclusion, our comprehensive allelotype analyses have unveiled and confirmed a total of 20 possible TSG loci that could be involved in the development of HNSCC. These results provide useful clues for identification of putative TSGs involved in HNSCC by fine mapping of the suspected regions and subsequent analysis for functional genes.
authors.affiliation: Department of Otolaryngology, Graduate School of Medicine and Dentistry, Okayama University, Shikata-cho, Okayama, Japan.
authors.forename: Levent Bekir
authors.initials: LB
authors.lastname: Beder
authors.forename: Mehmet
authors.initials: M
authors.lastname: Gunduz
authors.forename: Mamoru
authors.initials: M
authors.lastname: Ouchida
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Fukushima
authors.forename: Esra
authors.initials: E
authors.lastname: Gunduz
authors.forename: Sachio
authors.initials: S
authors.lastname: Ito
authors.forename: Akiko
authors.initials: A
authors.lastname: Sakai
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nagai
authors.forename: Kazunori
authors.initials: K
authors.lastname: Nishizaki
authors.forename: Kenji
authors.initials: K
authors.lastname: Shimizu
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002874:Chromosome Mapping; D005819:Genetic Markers; D016678:Genome; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats / Q000235:genetics
chemical_list: D005819:Genetic Markers