title: Positively charged sequences of human papillomavirus type 16 capsid proteins are sufficient to mediate gene transfer into target cells via the heparan sulfate receptor.
abstract: Using synthetic peptides we have shown that positively charged sequences present at the C terminus of the L1 protein and the N and C termini of the L2 protein of human papillomavirus type 16 (HPV-16) bind to both DNA and heparan sulfate receptors. Moreover, these short amino acid sequences are sufficient to mediate gene transfer in COS-7 cells. The L1 proteins of other HPVs were shown to contain one or two DNA- and heparin-binding sequences that have the capacity to transfer genes. These DNA-binding sequences also recognized the enhancing packaging sequence of bovine papillomavirus type 1. The results suggest that the L2 protein could participate in DNA packaging during maturation of virions.
authors.affiliation: Laboratoire de Virologie Moléculaire, INSERM EMIU 00-10 and USC INRA, Faculté des Sciences Pharmaceutiques 'Philippe Maupas', 31 avenue Monge, 37200 Tours, France.
authors.forename: Latifa
authors.initials: L
authors.lastname: Bousarghin
authors.affiliation: Laboratoire de Virologie Moléculaire, INSERM EMIU 00-10 and USC INRA, Faculté des Sciences Pharmaceutiques 'Philippe Maupas', 31 avenue Monge, 37200 Tours, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Touzé
authors.affiliation: Laboratoire de Virologie Moléculaire, INSERM EMIU 00-10 and USC INRA, Faculté des Sciences Pharmaceutiques 'Philippe Maupas', 31 avenue Monge, 37200 Tours, France.
authors.forename: Alba-Lucia
authors.initials: AL
authors.lastname: Combita-Rojas
authors.affiliation: Laboratoire de Virologie Moléculaire, INSERM EMIU 00-10 and USC INRA, Faculté des Sciences Pharmaceutiques 'Philippe Maupas', 31 avenue Monge, 37200 Tours, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Coursaget
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002213:Capsid / Q000737:chemistry* / Q000378:metabolism; D036022:Capsid Proteins; D004268:DNA-Binding Proteins / Q000378:metabolism; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006497:Heparan Sulfate / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D027383:Papillomaviridae / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014162:Transfection; D014771:Virion / Q000235:genetics / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D004268:DNA-Binding Proteins; D006497:Heparan Sulfate; D009856:Oncogene Proteins, Viral; D010455:Peptides; D011956:Receptors, Cell Surface; C063316:L2 protein, Human papillomavirus type 16; C063317:L1 protein, Human papillomavirus type 16