title: Molecular identification of Paracoccidioides brasiliensis by 5' nuclease assay.
abstract: A PCR assay based on the 5' nuclease assay using a fluorescent probe derived from the sequence of the gene coding for the 43,000-Da (gp43) antigen was developed to detect Paracoccidioides brasiliensis. The assay could detect at least 10 copies of this DNA sequence, providing efficient accuracy to be useful for diagnosis of paracoccidioidomycosis.
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Camile P
authors.initials: CP
authors.lastname: Semighini
authors.forename: Zoilo P
authors.initials: ZP
authors.lastname: de Camargo
authors.forename: Rosana
authors.initials: R
authors.lastname: Puccia
authors.forename: Maria Helena S
authors.initials: MH
authors.lastname: Goldman
authors.forename: Gustavo H
authors.initials: GH
authors.lastname: Goldman
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics; D015342:DNA Probes; D005092:Exonucleases / Q000378:metabolism*; D005453:Fluorescence; D005800:Genes, Fungal / Q000235:genetics; D016533:Mycological Typing Techniques / Q000379:methods*; D010228:Paracoccidioides / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D000951:Antigens, Neoplasm; D015342:DNA Probes; C107095:tumor-associated antigen, gp43; D005092:Exonucleases