title: Chromosomal arm replacement in Streptomyces griseus.
abstract: UV irradiation of Streptomyces griseus 2247 yielded a new chromosomal deletion mutant, MM9. Restriction and sequencing analysis revealed that homologous recombination between two similar lipoprotein-like open reading frames, which are located 450 and 250 kb from the left and right ends, respectively, caused chromosomal arm replacement. As a result, new 450-kb terminal inverted repeats (TIRs) were formed in place of the original 24-kb TIRs. Frequent homologous recombinations in Streptomyces strains suggest that telomere deletions can usually be repaired by recombinational DNA repair functioning between the intact and deleted TIR sequences on the same chromosome.
authors.affiliation: Department of Molecular Biotechnology, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Uchida
authors.forename: Mariko
authors.initials: M
authors.lastname: Miyawaki
authors.forename: Haruyasu
authors.initials: H
authors.lastname: Kinashi
mesh_terms: D001483:Base Sequence; D002872:Chromosome Deletion; D007446:Chromosome Inversion; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004260:DNA Repair; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D013305:Streptomyces griseus / Q000235:genetics*; D020079:Terminal Repeat Sequences