Visualizing chromatin and chromosomes in living cells.
The dynamic organization of eukaryotic genomes in cell nuclei recently came into the focus of research interest. The kinetics of genome dynamics can be addressed only by approaches involving live cell microscopy. Different methods are available to visualize chromatin, specific chromatin fractions, or individual chromosome territories within nuclei of living mammalian cells. Appropriate labeling procedures as well as cell chamber systems and important controls for live cell microscopy are described.
Zink
Daniele
D
Department Biologie 2, Universität München (LMU), Goethestrasse 31, 80336, Munich, Germany. Dani.Zink@lrz.uni-muenchen.de
Sadoni
Nicolas
N
Stelzer
Ernst
E
D000818:Animals; D002843:Chromatin / Q000502:physiology / Q000648:ultrastructure*; D002875:Chromosomes / Q000502:physiology / Q000648:ultrastructure*; D005057:Eukaryotic Cells / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*
D002843:Chromatin