[Part of biological activity of peptidoglycan from Streptococcus lactis SB900].
Peptidoglycan of Streptococcus lactis SB900(LABPG) was isolated. It's chemical composition was analyzed and part of biological activity was examined. The PG contained 9.84% protein, 0.871 mumol/m NAG, 1.14 mumol/mg NAM. The amino acids of relatively high concentrations were Ala, Glu, Asp and their concentrations were 1.046, 0.775, 0.304 mumol/mg respectively. Using mice as subject, the animal experiment confirmed that LABPG was non-toxic and safe. Effect of i.p. LABPG 0.5 mg/mouse on the phagocytic function were studied. It was suggested that the phagocytic activity of PM phi had markedly enhanced, the activity of serum lysozyme was increased significantly. YC-Rosette experiment suggested that the activity of C3b receptors of PM phi were increased and the YC-Rosette forming ratio were higher than controls. The difference was significant by statistical analysis. Therefore, it is considered that LABPG was able to activate M phi and improve immune function in mice.
Meng
F
F
State Key Laboratory of Microbil Technology, Shandong University, Jinan 250100.
Ma
G
G
Kong
J
J
D000818:Animals; D005260:Female; D013294:Lactococcus lactis / Q000737:chemistry*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D009113:Muramidase / Q000097:blood; D010457:Peptidoglycan / Q000737:chemistry / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects*; D017463:Receptors, Complement 3b / Q000378:metabolism; D012397:Rosette Formation
D010457:Peptidoglycan; D017463:Receptors, Complement 3b; D009113:Muramidase