title: Candidate markers for stem and early progenitor cells, Musashi-1 and Hes1, are expressed in crypt base columnar cells of mouse small intestine.
abstract: Musashi-1, a neural RNA-binding protein, is important for maintaining neural stem cells. Both Musashi-1 and Hes1, a transcriptional factor regulated by Musashi-1, are expressed in the small intestine. Here we show that Musashi-1 is present in a few epithelial cells just above the Paneth cells in the small intestinal crypt, the putative position of stem cells, whereas Hes1 is expressed in lower crypt cells just above the Paneth cells, including Musashi-1-positive cells. Musashi-1 and Hes1 were not expressed in Paneth cells. Notably, Musashi-1 and Hes1 were coexpressed in the crypt base columnar cells located between the Paneth cells. These findings suggest that not only the cells just above Paneth cells but also the crypt base columnar cells between the Paneth cells have stem cell characteristics.
authors.affiliation: Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, 54 Shogoin-Kawara-cho, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Takahisa
authors.initials: T
authors.lastname: Kayahara
authors.forename: Mitsutaka
authors.initials: M
authors.lastname: Sawada
authors.forename: Shigeo
authors.initials: S
authors.lastname: Takaishi
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Fukui
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Seno
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Fukuzawa
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Suzuki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hiai
authors.forename: Ryoichiro
authors.initials: R
authors.lastname: Kageyama
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Chiba
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D015415:Biomarkers / Q000032:analysis; D002460:Cell Line; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007421:Intestine, Small / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009928:Organ Specificity; D019879:Paneth Cells / Q000166:cytology; D012333:RNA, Messenger / Q000096:biosynthesis; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D000072056:Transcription Factor HES-1; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D015415:Biomarkers; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D000072056:Transcription Factor HES-1; D014157:Transcription Factors; C489734:Hes1 protein, mouse; C489735:Hes1 protein, rat; C491297:MSI1 protein, human; C491296:Msi1 protein, rat; C104786:Msi1h protein, mouse; C078493:HES1 protein, human