Characterization of a novel Drosophila melanogaster acylphosphatase.
Analysis of the Drosophila melanogaster EST database led to the characterization of a novel acylphosphatase (AcPDro2). This is coded by the CG18505 (Acyp2) gene and is clearly distinct from a previously described AcPDro coded by the CG16870 (Acyp) gene from D. melanogaster. The two proteins show a 60% homology with both vertebrate isoenzymes. All the residues involved in the catalytic mechanism are conserved. AcPDro2 is a stable enzyme with a correct globular folded structure. Its activity on benzoylphosphate shows higher K(cat) but lower K(m) with respect to AcPDro. It is possible that AcPDro and AcPDro2 genes are not the direct ancestor of MT and CT vertebrate isoenzymes.
Degl'Innocenti
Donatella
D
Dipartimento di Scienze Biochimiche, Università degli Studi di Firenze, Viale Morgagni 50, 50134 Firenze, Italy. donatella.deglinnocenti@unifi.it
Ramazzotti
Matteo
M
Marzocchini
Riccardo
R
Chiti
Fabrizio
F
Raugei
Giovanni
G
Ramponi
Giampietro
G
D017766:Acid Anhydride Hydrolases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001565:Benzoates / Q000737:chemistry; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics*; D004331:Drosophila melanogaster; D004789:Enzyme Activation; D004795:Enzyme Stability; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D014508:Urea / Q000737:chemistry; D000097024:Acylphosphatase
D017766:Acid Anhydride Hydrolases; D001565:Benzoates; D029721:Drosophila Proteins; D014508:Urea; D000097024:Acylphosphatase; C012217:benzoyl phosphate; C471291:Acyp2 protein, Drosophila