[In vivo distribution and expression after skin injury of fibronectin splicing variants].
OBJECTIVE
In order to develop new criteria and to select positive controls for forensic medicine, experiments on in vivo distribution and expression after skin wound of fibronectin splicing variants, EIIIA and EIIIB were studied.


METHODS
Rat tissues were homogenized to extract total RNA which was then amplified by RT-PCR technique.


RESULTS
(1) EIIIA+ and EIIIB+ of normal skin were not expressed, whereas EIIIA+ after 18 hours' injury was expressed abundantly. No obvious charge of EIIIB+ was detected. The differences between results of using two methods of injury were not significant. (2) The distribution of fibronectin variants in different adult organs were in the following order: brain > heart > liver.


CONCLUSIONS
EIIIA+ may be used as a sensitive criterion for research of skin injury.
Chen
Y J
YJ
Institute of Forensic Science, Ministry of Justice, Shanghai 200063. yijiuchen@yahoo.com.cn
Liu
N G
NG
Huang
X H
XH
Li
L
L
D000818:Animals; D000831:Animals, Newborn; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism*; D012326:RNA Splicing; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000293:injuries* / Q000378:metabolism*
D005353:Fibronectins