[Enantioseparation of twelve pharmaceutical racemates with high performance capillary electrophoresis using L-leucine as chiral selector].
A rapid enantiomeric separation method using L-leucine as chiral selector was established. Capillary zone electrophoresis (CZE) has been used for the enantiomeric separation of twelve pharmaceutical racemates with bare fused silica capillary and employing L-leucine as chiral selector. The enantiomeric resolution was influenced by L-leucine concentration and pH of background electrolyte (BGE). The effects of the BGE types and concentrations on the enantiomeric separation were also investigated. The results showed that in the solution containing 50 mmol/L borax and 70 mmol/L L-leucine (pH 9.0), all the twelve drugs were on baseline separated in less than 11 minutes.
Lin
X L
XL
Department of Chemical Engineering, Shandong Institute of Light Industry, Jinan 250100, China.
Li
G B
GB
Zhu
C F
CF
Wu
P
P
Guan
Y F
YF
D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D005277:Fenfluramine / Q000032:analysis / Q000302:isolation & purification; D007930:Leucine; D008801:Mexiletine / Q000032:analysis* / Q000302:isolation & purification; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000302:isolation & purification; D012822:Silicon Dioxide; D013237:Stereoisomerism; D014700:Verapamil / Q000032:analysis* / Q000302:isolation & purification
D004364:Pharmaceutical Preparations; D008801:Mexiletine; D005277:Fenfluramine; D012822:Silicon Dioxide; D014700:Verapamil; D007930:Leucine