[Determination of glycyrrhetic acid in toothpaste by reversed-phase high performance liquid chromatography (RP-HPLC)].
A method for determining glycyrrhetic acid in toothpaste by RP-HPLC is described. The operating conditions were YWG-C18 column (4.0 mm i.d. x 250 mm, 10 microns) at room temperature, V(methanol):V(0.01 mol/L KH2PO4) = 85:15 (pH 3.0) as mobile phase with flow rate of 1.0 mL/min and UV detection at 254 nm. Glycyrrhetic acid in toothpaste samples were extracted with methanol. The average recoveries of glycyrrhetic acid were 99.61%-101.67%. The relative standard deviations were 1.85%-3.16%. The method is simple, rapid and accurate.
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Institute of Applied Chemistry, Nanchang University, Nanchang 330047, China.
D000287:Administration, Topical; D000893:Anti-Inflammatory Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006034:Glycyrrhetinic Acid / Q000032:analysis*; D014100:Toothpastes / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D014100:Toothpastes; D006034:Glycyrrhetinic Acid