[Regulation of XW630 on TGF-beta 1 and collagen I gene expression in osteoblasts].
OBJECTIVE
The purpose of this part of study was to investigate the mechanism of a new anti-osteoporotic drug, XW630 on promoting osteogenic activity.


METHODS
Total RNA were isolated from osteoblasts of the mice after the reaction with XW630 in 24 and 72 hours, that were hybridized with the extracted and digoxigenin-labeled TGF-beta 1 and the collagen I cDNA probes.


RESULTS
XW630 significantly promoted TGF-beta 1 and collagen I gene expression in osteoblasts, and this drug was superior than other two kinds of estrogen in promoting osteogenic activity (P < 0.05).


CONCLUSIONS
XW630 promote osteogenic activity of osteoblasts, probably by enhancing the mRNA expression of TGF-beta 1 and collagen I.
Fei
W
W
Department of Oral and Maxillofacial Sugery, Sichuan Provincial People's Hospital.
Wang
D
D
Zheng
H
H
Weng
L
L
Deng
L
L
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D008297:Male; D010006:Osteoblasts / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects*; D010879:Piperazines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013752:Tetracycline / Q000494:pharmacology*; D013754:Tetracyclines; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D053773:Transforming Growth Factor beta1
C428143:2-(3-estrone-N-ethyl-piperazine-methyl)tetracycline; D010879:Piperazines; D012333:RNA, Messenger; D013754:Tetracyclines; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D003094:Collagen; D013752:Tetracycline