Crystallization and preliminary X-ray crystallographic analysis of Sclerotium rolfsii lectin.
Sclerotium rolfsii lectin (SRL), from the soil-borne phytopathogenic fungus S. rolfsii, has been crystallized. SRL crystals were grown by the hanging-drop vapour-diffusion method using an MPD-ammonium acetate mixture in Tris-HCl buffer pH 8.5. A complete data set from a single crystal at 100 K was collected to 1.1 A resolution using synchrotron radiation. Preliminary crystallographic analysis showed that the crystals belong to the tetragonal space group P4(2)2(1)2, with unit-cell parameters a = b = 99.81, c = 63.99 A and two molecules per asymmetric unit.
Leonidas
Demetres D
DD
Institute of Biological Research and Biotechnology, The National Hellenic Research Foundation, 48 Vas. Constantinou Ave, 11635 Athens, Greece. ddl@eie.gr
Swamy
Bale M
BM
Bhat
Anuradha G
AG
Inamdar
Shashikala R
SR
Kosmopoulou
Magda N
MN
Chrysina
Evangelia D
ED
Oikonomakos
Nikos G
NG
D001203:Ascomycota / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*
D005656:Fungal Proteins; D037102:Lectins