[Genetic diversity of rhizobia of Medicago edgeworthii by AFLP and RFLP analysis].
Two hundred and ninety one isolates of rhizobia sampled from root nodules of Medicago edgeworthii which came from Yun Nan Province in china were analyzed by amplified restriction fragment polymorphism(AFLP) technique. According to the AFLP banding patterns, the results showed most of these isolates were genetically diversity. Ninety isolates were selected as representing the diversity among the 291 isolates. The 90 isolates were clustered into three groups at the level of similarity of 79% by computer analysis of the data. Additional representative isolates and reference strains were identified by restriction fragment length polymorphism (RFLP) analysis of PCR-Amplified 16S rDNA. Two different 16S rDNA PCR-RFLP types were found, which indicated that the isoltes were phylogentically closely related to Rhizobium mongolense.
Feng
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Soil and Fertilizers Institute, CAAS, Beijing 100081.
D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D029902:Medicago / Q000382:microbiology*; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012230:Rhizobiaceae / Q000235:genetics*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S