[Pyruvate oxidase gene from Streptococcus sanguis: molecular cloning and sequence analysis of the gene].
OBJECTIVE
To clone and sequence the gene of pyruvate oxidase (Sopox) from Streptococcus sanguis.


METHODS
The PCR primers for Sopox gene were designed and synthesized according to the sequence of pyruvate oxidase (spxB) gene of S. pneumonia. The amplified PCR product was cloned into pUC18 and then subcloned into M13mp18 and M13mp19. The DNA sequence of the gene was analyzed.


RESULTS
Sopox gene was successfully amplified from S. sanguis ATCC10557. The nucleotide sequence of the whole gene was revealed to be 1788 base pairs with one open reading frame coding pyruvate oxidase with 591 amino acid residuals.


CONCLUSIONS
The clone and DNA sequence of Sopox gene were obtained which could serve as a foundation on which to elucidate the molecular mechanisms of hydrogen peroxide production and its regulation by oral streptococci.
Hou
B
B
School of Stomatology, WCUMS, Chengdu 610041, China.
Zhang
R
R
Zhang
J
J
Qian
W
W
Zhang
Y
Y
D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011771:Pyruvate Oxidase / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013296:Streptococcus pneumoniae / Q000235:genetics; D013298:Streptococcus sanguis / Q000145:classification / Q000235:genetics* / Q000378:metabolism
D011771:Pyruvate Oxidase