[Quantitative assessment of the processes of insulin synthesis and secretion in B-endocrinocytes of pancreatic islets].
Immunofluorescent methods allowing the detection of different hormones in endocrine cells, are widely used in many works, however, their application is usually limited to the description of qualitative criteria, while the reactions of indirect immunofluorescence permit their quantitative assessment. The purpose of the present work was the definition of the criteria for the quantitative assessment of the activities of the processes of insulin synthesis and secretion in pancreatic B-endocrinocytes by examination of changes of their fluorescence using classical activation of these cells by glucose. The investigations performed indicate that the main parameters of activity of insulin synthesis and secretion in pancreatic B-endocrinocytes are the area occupied by immunoreactive material, its concentration and heterogeneity. As the secretory response of pancreatic B-endocrinocytes is of variable intensity, the classification of these cells into groups with different activity of the processes of insulin synthesis and secretion is proposed. This approach enables the quantitative assessment of the action of different insulotropic agents, opening promising prospects for their further investigation.
Kolesnik
Iu M
IuM
Department of Pathological Physiology, State Medical University, Zaporozhye.
Orlovskiĭ
M A
MA
Abramov
A V
AV
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D019084:Fluorescent Antibody Technique, Indirect; D005947:Glucose / Q000494:pharmacology; D007274:Injections, Intraperitoneal; D007328:Insulin / Q000096:biosynthesis* / Q000097:blood / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008856:Microscopy, Fluorescence; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D007328:Insulin; D005947:Glucose