[Cloning and expression of caiDE genes in E. coli BL21(DE3)].
The caiDE genes for carnitine racemase and its relative factor were cloned into pUC18 from the chromosomal DNA of E. coli MC4100 by shot-gun approach and have been verified by sequence analysis. The target plasmid was named as pJX393 and subcloned to form a transcription plasmid pDSW2. After its transformation into BL21(DE3) strain and induction by IPTG, the recombinant strain containing pDSW2 expressed two apparent protein bands on SDS-PAGE, whose molecular weight were 30 kD and 24 kD individually.
Jiang
J
J
State Key Laboratory of Bio-Reactorand Engineering, ECUST, Shanghai 200237.
Duan
S
S
Zhang
H
H
D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D010957:Plasmids; D019998:Racemases and Epimerases / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D019998:Racemases and Epimerases; C091404:caiD protein, E coli