Sodium selenite, dietary micronutrient, prevents the lymphocyte DNA damage induced by N-nitrosodiethylamine and phenobarbital promoted experimental hepatocarcinogenesis.
Selenium (Se), a micronutrient, has a long history in chemoprevention of mammary and colon cancers in rodent models. Se is a current clinical trial, having shown promise in prevention of prostate and other human cancers. The mechanisms involved in the in vivo anti-carcinogenic activity of Se remain to be elucidated. In the present study, we examined the effect of sodium selenite supplementation in lymphocytes, obtained from hepatoma bearing rats on DNA damage in correlation with oxidative stress. In addition, this study examined the supplementation of Se at 4-ppm levels in the form of sodium selenite either before initiation or during initiation and/or promotion phase's increases lymphocyte Se concentrations. This in turn improves lymphocyte resistance to oxidative stress and protection against the lymphocytes DNA damage. Supplementation of Se increased lymphocyte Se concentration and reduced lymphocytes DNA damage as determined by single cell gel electrophoresis. The enzymatic antioxidants such as superoxide dismutase, glutathione peroxidase, and catalase were found to be decreased while the thiobarbituric acid reactive substances level was increased in the lymphocytes of hepatoma bearing rats. Furthermore, the reactive oxygen species such as superoxide radicals and hydroxyl radicals were also found to be high in lymphocytes. Our present results explain the understanding of unique association between anti-peroxidative effect of Se and ultimately the capability of Se to prevent cancer.
Thirunavukkarasu
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Department of Medical Biochemistry, Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Chennai, India. tchinnasamy@hotmail.com
Sakthisekaran
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D000477:Alkylating Agents / Q000494:pharmacology / Q000633:toxicity; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology / Q000633:toxicity; D000975:Antioxidants / Q000378:metabolism; D004249:DNA Damage; D019587:Dietary Supplements; D004052:Diethylnitrosamine / Q000494:pharmacology* / Q000633:toxicity; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation; D008114:Liver Neoplasms, Experimental; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008297:Male; D018977:Micronutrients; D018384:Oxidative Stress; D010634:Phenobarbital / Q000494:pharmacology* / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D018038:Sodium Selenite / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*
D000477:Alkylating Agents; D000927:Anticonvulsants; D000975:Antioxidants; D005609:Free Radicals; D018977:Micronutrients; D004052:Diethylnitrosamine; D018038:Sodium Selenite; D010634:Phenobarbital