[Construction of cDNA library of Mortierella and screening of delta 9 fatty acid desaturase cDNA sequence].
Mortierella species have potential for fermentative production of polyunsaturated fatty acids including gamma-linolenic acid, Arachidonic acid and EPA, etc. In order to clone genes encoding enzymes in the unsaturated fatty acid biosynthetic pathway, cDNA library of Mortierella was constructed using lambda gt 10 vector. Using cDNA encoding conserved region of delta 9 fatty acid desaturase gene as probe, Mortierella cDNA library was screened. After two rounds of screening one positive clone was identified which has insert length of larger than 1.6 kb.
Zhang
Y
Y
College of Food and Bioengineering, South China University of Technology, Guangzhou 510641.
Lin
W
W
Yao
R
R
Bao
S
S
Zheng
X
X
D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D044943:Fatty Acid Desaturases / Q000235:genetics*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis; D015723:Gene Library; D020094:Mortierella / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase
D018076:DNA, Complementary; D005231:Fatty Acids, Unsaturated; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; C029627:delta-9 fatty acid desaturase