title: Expression of P53 during lens epithelial cell apoptosis induced by ultraviolet.
abstract: The apoptosis of lens epithelial cells (LECs) induced by ultraviolet and the expression of P53 were investigated. Wistar rats received 100 mW/m2 ultraviolet irradiation (UVR) (lambda = 280 nm-315 nm) for 15 min. One, 6, 24 h after irradiation the lens capsules were dissected. The percentages of apoptotic cells were evaluated by the TdT-dUTP terminal nick-end labeling (TUNEL) technique and the expression of P53 was detected by using immunohistochemical assay. The results showed that the percentages of TUNEL-positive nuclei at 24 h after irradiation was significantly higher than in the control group and those 1 h, 6 h after irradiation. The percentages of P53-positive cells at 6 h, 24 h after irradiation were significantly higher than in the control group and those 1 h after irradiation. It was concluded that UVR could induce the apoptosis of lens epithelial cell. The expression of P53 might be responsible for the apoptosis of lens epithelial cells.
authors.affiliation: Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Teconology, Wuhan 430030.
authors.forename: X
authors.initials: X
authors.lastname: Sun
authors.forename: W
authors.initials: W
authors.lastname: Zou
authors.forename: C
authors.initials: C
authors.lastname: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D002386:Cataract / Q000209:etiology; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D007908:Lens, Crystalline / Q000378:metabolism / Q000473:pathology*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53