Determination of corticosterone in mouse plasma by a sweeping technique using micellar electrokinetic chromatography.
The separation and on-line concentration of corticosterone in mouse blood was achieved by means of capillary electrophoresis/UV absorbance using sodium dodecyl sulfate (SDS) as a surfactant. The procedure involved the use of an on-line sample concentration method by sweeping-micellar electrokinetic chromatography (sweeping-MEKC). Optimal on-line concentration and separation conditions were determined. The detection limit for this method was 5 ng/ml (S/N=3) and photodiode array detection at 247 nm was used for identification. For the analysis of actual samples, corticosterones from blood samples of a non-stressed and stressed mouse were determined. The results show that only a minor amount of corticosterone was produced by a non-stressed mouse, whereas a significant amount was present in the blood sample from a stressed mouse. The method developed here can be used to examine corticosterone levels as a marker of stress in test animals and may also be used for estimating the effect of stress-release medications.
Wu
Chun-Hsueh
CH
Department of Chemistry, National Taiwan Normal University, 88 Sec 4, Tingchow Road, Taipei, Taiwan.
Chen
Mei-Chuan
MC
Su
An-Kai
AK
Shu
Pin-Yan
PY
Chou
Shiu-Huey
SH
Lin
Cheng-Huang
CH
D015069:18-Hydroxycorticosterone / Q000097:blood; D000818:Animals; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary; D003345:Corticosterone / Q000097:blood*; D007202:Indicators and Reagents; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009862:Online Systems; D012967:Sodium Dodecyl Sulfate; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents
D002021:Buffers; D007202:Indicators and Reagents; D012997:Solvents; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D015069:18-Hydroxycorticosterone; D003345:Corticosterone