[Determining the distribution of Mutans Streptococci in human dental plaque by monoclonal antibody against SA I/II and SPAa of Mutans Streptococci].
OBJECTIVE
To explore the possibility of detecting the distribution of Mutans Streptococci in human dental plaque by Monoclonal antibody (WC2E10c, WC3A6d) against SA I/II and SPAa of Mutans Streptococci, which was prepared in our lab.


METHODS
60 subjects were divided into the experimental group and the control group. The distributions of Mutans Streptococci (serotype c and d) in dental plaques were detected in 60 subjects by the clonal blot technique. Positive colonies were examined by bacterial morphology, serology, biochemistry and SDS-PAGE.


RESULTS
Bacterial colony was significantly different between the experimental group and the control group with eye observation. Colonies of the experimental group were deep brown and easily differentiated, but colonies of control group were white. Brown colonies were proved to be Mutans Streptococci (serotype c and d) by using assay of bacterial morphology, serology, biochemistry and SDS-PAGE, but white colonies were not.


CONCLUSIONS
McAbs (WC2E10c, WC3A6d) were shown high specificity in Mutans Streptococci (Serotype c and d) of human dental plaques.
Wen
L
L
Department of Oral Medicine, 309th Hospital of PLA, Beijing.
Zhang
C
C
Yue
S
S
D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D002648:Child; D003773:Dental Plaque / Q000382:microbiology*; D006801:Humans; D008875:Middle Aged; D013295:Streptococcus mutans / Q000276:immunology*; D010948:Viral Plaque Assay
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal