[Protective effect of ginsenosides on human peritoneal mesothelial cell against lactate-based peritoneal dialysis solutions--induced injury in vitro].
OBJECTIVE
The aim of this research was to observe the influence of ginsenosides (GS) on viability and proliferation of human peritoneal mesothelial cell (HPMC) which were inhibited by L-PDS in vitro.


METHODS
Mesothelial cells were isolated from human omental specimens by trypsin disaggregation and established a stable HPMC culture model. The viability and capacity of proliferation of HPMC were assessed by lactate dehydrognase (LDH) release and tetrazolium salt colorimetry assay (MTT assay).


RESULTS
Compared with the control group, the release of LDH in L-PDS groups (2.5%, 4.25% glucose) significantly increased. But there was no difference between the two glucose concentrations. As HPMC pre-exposed to L-PDS prolonged, the viability of cells decreased gradually. GS decreased the release of LDH and increased the cells viability (P < 0.01), as well as enhanced the proliferation of HPMC.


CONCLUSIONS
The results suggest that GS has some protective effects on cell viability and proliferation of HPMC inhibited by L-PDS in vitro.
Ye
Y
Y
Department of Geriatrics, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Peng
Y M
YM
Liu
F Y
FY
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D015314:Dialysis Solutions / Q000009:adverse effects*; D004848:Epithelium; D036145:Ginsenosides / Q000494:pharmacology*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007773:Lactates / Q000009:adverse effects; D009852:Omentum / Q000166:cytology; D010537:Peritoneum / Q000166:cytology*
D015314:Dialysis Solutions; D036145:Ginsenosides; D007773:Lactates; D007770:L-Lactate Dehydrogenase