title: IL-8 expression and its possible relationship with estrogen-receptor-negative status of breast cancer cells.
abstract: Estrogen-receptor (ER) status is an important parameter in breast cancer management as ER-positive breast cancers have a better prognosis than ER-negative tumors. This difference comes essentially from the lower aggressiveness and invasiveness of ER-positive tumors. Here, we demonstrate, that interleukin-8 (IL-8) was clearly overexpressed in most ER-negative breast, ovary cell lines and breast tumor samples tested, whereas no significant IL-8 level could be detected in ER-positive breast or ovarian cell lines. We have also cloned human IL-8 from ER-negative MDA-MB-231 cells, and we show that IL-8 produced by breast cancer cells is identical to monocyte-derived IL-8. Interestingly, the invasion potential of ER-negative breast cancer cells is associated at least in part with expression of IL-8, but not with IL-8 receptor levels. Moreover, IL-8 increases the invasiveness of ER-positive breast cancer cells by two fold, thus confirming the invasion-promoting role of IL-8. On the other hand, exogenous expression of estrogen receptors in ER-negative cells led to a decrease of IL-8 levels. In summary, our data show that IL-8 expression is negatively linked to ER status of breast and ovarian cancer cells. We also support the idea that IL-8 expression is associated with a higher invasiveness potential of cancer cells in vitro, which suggests that IL-8 could be a novel marker of tumor aggressiveness.
authors.affiliation: INSERM U540, Molecular and Cellular Endocrinology of Cancers, Montpellier, France.
authors.forename: Ariane
authors.initials: A
authors.lastname: Freund
authors.forename: Corine
authors.initials: C
authors.lastname: Chauveau
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Brouillet
authors.forename: Annick
authors.initials: A
authors.lastname: Lucas
authors.forename: Matthieu
authors.initials: M
authors.lastname: Lacroix
authors.forename: Anne
authors.initials: A
authors.lastname: Licznar
authors.forename: Françoise
authors.initials: F
authors.lastname: Vignon
authors.forename: Gwendal
authors.initials: G
authors.lastname: Lazennec
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015197:Carcinogenicity Tests; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020382:Interleukin-18 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D011960:Receptors, Estrogen / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D020382:Interleukin-18; D011960:Receptors, Estrogen