[High performance liquid chromatographic determination of domoic acid in shellfish samples].
Domoic acid in shellfish samples was determined by HPLC with DAD/UV detector at 242 nm. Samples were extracted with methanol-water followed by clean-up of the extracts with strong anion exchange solid phase extraction cartridge(3 mL LC-SAX). Zorbax SB-C18 column, 150 mm x 4.6 mm i.d., and mobile phase of acetonitrile-0.1% aqueous trifluoroacetic acid(13:87, V/V) were used for the assay. The quantitative analysis was performed with external standard. The calibration curve of domoic acid was linear in the range of 1.0 m/L-25.0 mg/L and the detection limit was ca. 0.2 microgram/g.
Wei
F
F
Liaoning Entry-Exit Inspection and Quarantine Bureau of China, Dalian 116001, China.
Cheng
F
F
Gong
J H
JH
Tang
S T
ST
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007474:Ion Exchange; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000032:analysis*; D012758:Shellfish / Q000032:analysis*
C012301:domoic acid; D007608:Kainic Acid