title: The pathway of dephosphorylation of myo-inositol hexakisphosphate by phytate-degrading enzymes of different Bacillus spp.
abstract: The pathway of dephosphorylation of myo-inositol hexakisphosphate by the phytate-degrading enzymes of Bacillus subtilis 168, Bacillus amyloliquefaciens ATCC 15841, and Bacillus amyloliquefaciens 45 was established using a combination of high-performance ion chromatography analysis and kinetic studies. The data demonstrate that all the Bacillus phytate-degrading enzymes under investigation dephosphorylate myo-inositol hexakisphosphate by sequential removal of phosphate groups via two independent routes; the routes proceed via D-Ins(1,2,4,5,6)P5 to Ins(2,4,5,6)P4 to finally Ins(2,4,6)P3 or D-Ins(2,5,6)P3 and via D-Ins(1,2,4,5,6)P5 to D-Ins(1,2,5,6)P4 to finally D-Ins(1,2,6)P3. The resulting myo-inositol trisphosphate D-Ins(1,2,6)P3 was degraded via D-Ins(2,6)P2 to finally Ins(2)P after prolonged incubation times in combination with increased enzyme concentration.
authors.affiliation: Centre for Molecular Biology, Federal Research Centre for Nutrition, Haid-und-Neu-Strasse 9, D-76131 Karlsruhe, Germany. ralf.greiner@bfe.uni-karlsruhe.de
authors.forename: Ralf
authors.initials: R
authors.lastname: Greiner
authors.forename: Adelazim
authors.initials: A
authors.lastname: Farouk
authors.forename: Marie Larsson
authors.initials: ML
authors.lastname: Alminger
authors.forename: Nils-Gunnar
authors.initials: NG
authors.lastname: Carlsson
mesh_terms: D010832:6-Phytase / Q000378:metabolism*; D001407:Bacillus / Q000145:classification / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D010766:Phosphorylation; D010833:Phytic Acid / Q000378:metabolism*
chemical_list: D010833:Phytic Acid; D010832:6-Phytase