[Establishment of transformation system of Streptomyces lincolnensis].
Transformation system of S. lincolnensis was established to improve lincomycin--producing strains by Gene retransformation Technology. Under optimal conditions, 10(11) protoplasts were prepared from 25 ml SM culture medium, and the regeneration frequency of protoplasts was 16%. The transformation frequency of pIJ702 was up to 5.5 x 10(5)/microgram DNA. The results revealed that gene manipulation in S. lincolnensis was facile as that in S. lividans.
Zhang
H
H
Department of Biochemical Engineering, East China University of Science and Technology, Shanghai 200237.
Yao
F
F
Ye
J
J
D003470:Culture Media; D018014:Gene Transfer Techniques; D011523:Protoplasts / Q000502:physiology*; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development; D014169:Transformation, Bacterial / Q000502:physiology*
D003470:Culture Media