[A method of morphological observation on the endothelialization of bioprosthetic heart valve materials in vitro].
In order to examine the endothelialization of yak pericardial materials in vitro, we used Wright staining and successfully observed the morphology, number and order of endothelial cells seeded on pericardial patches under light microscope. Compared with scanning electron microscopy, the method of light microscopy is simple, easy to do and may be carried out at any time. In laboratory, it brings much convenience in morphological observation on the endothelialization of biomaterials and therefore is worth recommendation.
Wei
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Department of Thoratic Surgery, Affiliated Hospital of North Sichuan Medical University, Nanchong 63700.
Shi
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Y
Wang
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D000818:Animals; D001698:Biomedical Engineering; D001705:Bioprosthesis; D002417:Cattle; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology*; D006350:Heart Valve Prosthesis; D066298:In Vitro Techniques; D008853:Microscopy / Q000379:methods; D010496:Pericardium / Q000033:anatomy & histology; D013194:Staining and Labeling / Q000379:methods