Alterations in Na+/H+ exchanger and Na+/HCO3- cotransporter immunoreactivities within the gerbil hippocampus following seizure.
In this study, a chronological and comparative analysis of the immunoreactivities of Na(+)/H(+) exchanger 1 (NHE1), Na(+)/HCO(3)(-) cotransporter (NBC) and Na(+)/Ca(2+) exchanger (NCE) was conducted in order to identify the effects of spontaneous seizure on their protein expression levels using the gerbil model. The distribution of NHE1 and NBC immunoreactivity in the hippocampus of seizure-resistant (SR) gerbils was similar to that observed in the pre-seizure group of seizure-sensitive (SS) gerbils. From 30 min to 3 h after the onset of the seizure, both NHE1 and NBC immunoreactivities were elevated in the hippocampus, as compared to the pre-seizure group of SS gerbils. At 6 h postictal, these immunoreactivities in the hippocampus had reduced to the pre-seizure level. However, NCE immunoreactivity within the hippocampus was unaltered. These findings suggest that the changes in both NHE1 and NBC immunoreactivity within the hippocampus following seizure may affect tissue excitability and play a role in the reduction of the seizure activity in the gerbil.
Kang
Tae-Cheon
TC
Department of Anatomy, College of Medicine, Hallym University, Chunchon, Kangwon-Do, 200-702, South Korea. tckang@hallym.ac.kr
An
Sung-Jin
SJ
Park
Seung-Kook
SK
Hwang
In-Koo
IK
Suh
Jun-Gyo
JG
Oh
Yang-Seok
YS
Bae
Jae Chun
JC
Won
Moo Ho
MH
D000818:Animals; D005849:Gerbillinae; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D012640:Seizures / Q000378:metabolism*; D027982:Sodium-Bicarbonate Symporters / Q000276:immunology / Q000378:metabolism*; D019831:Sodium-Calcium Exchanger / Q000276:immunology / Q000378:metabolism*; D017923:Sodium-Hydrogen Exchangers / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors
D027982:Sodium-Bicarbonate Symporters; D019831:Sodium-Calcium Exchanger; D017923:Sodium-Hydrogen Exchangers; C069139:growth factor-activatable Na-H exchanger NHE-1