[The effect of injuries on Fn synthesized by FBs and its implication in wound timing].
cFn synthesized by FBs around the wound was detected by immunochemistry in vitro. Using the method of ELISA, The concentration of cFn in the culture media was observed. The concentration of cFn had no change within 30 minutes after injury, but started to increase after 1 hour of the injury and kept this trend within next five hours after injury. By static analysis, it can be obtained that the concentration of cFn in culture media is time-dependent after injury.
Shen
Y
Y
Department of Forensic Medicine, Shanghai Medical University, Shanghai 200032, PRC.
Zhao
Z
Z
Chen
Y
Y
Yan
Z
Z
Gu
Y
Y
Wu
W
W
Liao
Y
Y
D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology*; D005353:Fibronectins / Q000096:biosynthesis* / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000378:metabolism*; D055370:Lung Injury; D012044:Regression Analysis; D013997:Time Factors
D005353:Fibronectins