Trophic support of mouse inner ear by neural stem cell transplantation.
In the auditory system, efforts to reduce degeneration of spiral ganglion neurons have the immediate objective of improving clinical benefits of cochlear implants, which are small devices designed to stimulate spiral ganglion neurons electronically. Recent studies have indicated several neurotrophins can enhance survival of spiral ganglion neurons. However, the strategy for application of neurotrophins in inner ear is still a matter of debate. In this study, we examined the potential of cell therapy as a strategy for application of neurotrophins in the inner ear. Neural stem cells obtained from green fluorescent protein-transgenic mice were used as donor cells. Medium containing neural stem cells was injected into mouse inner ear. Histological analysis 4 weeks later revealed that transplant-derived cells survived in inner ear and that most transplant-derived cells in the cochlea had differentiated into glial cells. Moreover, expression of glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor was observed in transplant-derived cells. These findings indicate that transplantation of neural stem cells can be a useful strategy for application of neurotrophins in inner ear.
Iguchi
Fukuichiro
F
Department of Otolaryngology Head and Neck Surgery, Kyoto University Graduate School of Medicine, Japan.
Nakagawa
Takayuki
T
Tateya
Ichiro
I
Kim
Tae Soo
TS
Endo
Tsuyoshi
T
Taniguchi
Zenchi
Z
Naito
Yasushi
Y
Ito
Juichi
J
D000818:Animals; D016380:Brain Tissue Transplantation; D019208:Brain-Derived Neurotrophic Factor / Q000032:analysis; D002454:Cell Differentiation; D019070:Cell Lineage; D003051:Cochlea / Q000737:chemistry / Q000166:cytology*; D005260:Female; D016332:Fetal Tissue Transplantation; D051100:Glial Cell Line-Derived Neurotrophic Factor; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006085:Graft Survival; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins / Q000032:analysis; D009414:Nerve Growth Factors / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis; D009457:Neuroglia / Q000737:chemistry / Q000166:cytology; D009474:Neurons / Q000737:chemistry / Q000166:cytology; D012665:Semicircular Canals; D033581:Stem Cell Transplantation; D013687:Telencephalon / Q000166:cytology / Q000196:embryology; D016042:Transplantation, Heterotopic
D019208:Brain-Derived Neurotrophic Factor; C495040:Gdnf protein, mouse; D051100:Glial Cell Line-Derived Neurotrophic Factor; D005904:Glial Fibrillary Acidic Protein; D008164:Luminescent Proteins; D008869:Microtubule-Associated Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D049452:Green Fluorescent Proteins