title: Low presence of p53 abnormalities in H pylori-infected gastric mucosa and in gastric adenocarcinoma.
abstract: BACKGROUND
  Alterations of the p53 gene and/or its abnormal protein accumulation have been observed in gastric cancer and preneoplastic lesions. Our aim was to assess possible associations between different H. pylori strains and p53 abnormalities in patients with dyspepsia and with gastric cancer.
  
  
  METHODS
  Seventy-five dyspeptic patients and 40 patients with gastric adenocarcinoma entered the study. H. pylori status was determined by the rapid urease test, histology, and polymerase chain reaction (PCR) analysis. Overexpression of the p53 protein was evaluated by immunohistochemistry. Detection of p53 mutations was done by direct DNA sequencing.
  
  
  RESULTS
  Fifty-four of the 75 (72.0%) dyspeptic patients and 27 of the 40 (67.5%) gastric cancer patients showed H. pylori infection. Cytotoxin-associated gene ( cagA)-positive strains were found in 31 of the 54 (58%) dyspeptic patients and in 25 of the 27 (92.6%) neoplastic patients. As regards vacA, s2 strains showed the highest prevalence among dyspeptic patients (24 of 54 patients; 44.4%), whereas s1 strains were more expressed among cancer patients (23 of 27; 85.2%). Among the dyspeptic patients, 1 patient with duodenal ulcer showed p53 overexpression. Three mutations were identified by DNA sequencing: one in a patient with normal endoscopic findings and two in patients suffering from gastritis. Among the neoplastic patients, 16 subjects (40%) showed p53 overexpression (9 had diffuse-type and 7 intestinal-type cancer). Four mutations (10%) occurred in patients with intestinal-type gastric cancer. No association between p53 abnormalities (overexpression/mutation) and H. pylori infection was found in either group of patients.
  
  
  CONCLUSIONS
  These results lead us to hypothesize that H. pylori infection does not affect the p53 pattern in gastric mucosa. Moreover, mutations of the p53 gene do not seem to be a predominant event in gastric carcinogenesis, at least in our populations.
authors.affiliation: Laboratory of Biochemistry, Scientific Institute for Digestive Diseases, IRCCS "Saverio de Bellis", 70013 Castellana Grotte (Bari), Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Berloco
authors.forename: Francesco
authors.initials: F
authors.lastname: Russo
authors.forename: Filomena
authors.initials: F
authors.lastname: Cariola
authors.forename: Mattia
authors.initials: M
authors.lastname: Gentile
authors.forename: Piero
authors.initials: P
authors.lastname: Giorgio
authors.forename: Maria Lucia
authors.initials: ML
authors.lastname: Caruso
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Valentini
authors.forename: Giovanni
authors.initials: G
authors.lastname: Di Matteo
authors.forename: Alfredo
authors.initials: A
authors.lastname: Di Leo
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004269:DNA, Bacterial / Q000032:analysis; D004415:Dyspepsia / Q000235:genetics*; D005260:Female; D005753:Gastric Mucosa / Q000737:chemistry; D016481:Helicobacter Infections / Q000235:genetics*; D016480:Helicobacter pylori; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D016159:Tumor Suppressor Protein p53