Resolution of chiral drugs by liquid chromatography based upon diastereomer formation with chiral derivatization reagents.
Chiral derivatization reagents for resolution of biologically important compounds, such as chiral drugs by high-performance liquid chromatography (HPLC), based upon pre-column derivatization and diastereomer formation, are reviewed. The derivatization reagents for various functional groups, i.e., amine, carboxyl, carbonyl, hydroxyl and thiol, are evaluated in terms of reactivity, stability, wavelength, handling, versatility, sensitivity, and selectivity. The applicability of the reagents to the analyses of drugs and bioactive compounds are included in the text.
Toyo'oka
Toshimasa
T
School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan. toyooka@ys2.u-shizuoka-ken.ac.jp
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D004341:Drug Evaluation / Q000379:methods; D006801:Humans; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000138:chemical synthesis / Q000145:classification / Q000302:isolation & purification; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D013237:Stereoisomerism
D004364:Pharmaceutical Preparations