title: Activation of signal transducer and activator of transcription 3 in rat liver after heat shock and reperfusion stress.
abstract: Changes in transcription factors (TFs) accompany many types of cell stresses. By using electrophoretic mobility assays we show that the DNA binding of signal transducer and activator of transcription 3 (STAT3) is activated in rat liver by heat shock and ischemia-reperfusion. Northern blot and Western blot analysis reveal an increase of the mRNA and protein level of this transcription factor. Under both conditions the phosphorylation of pre-existing STAT3 is prompt and precedes the increase in the STAT3 protein. The activation: (1) is functional, i.e. is followed by the transcription of the target gene alpha(1)-acid glycoprotein (2) is strongly inhibited by pretreatment with the interleukin-1 receptor antagonist before heat shock but only slightly by pretreatment before ischemia-reperfusion (3) might, at least in part, be mediated by a cytokine cascade involving also interleukin-6. These results are consistent with the hypothesis that different kinds of stress can activate a number of common TFs.
authors.affiliation: Istituto di Patologia Generale dell'Università degli Studi di Milano, Centro di Studio sulla Patologia Cellulare del CNR, via Mangiagalli 31, 20133 Milan, Italy.
authors.forename: Lorenza
authors.initials: L
authors.lastname: Tacchini
authors.forename: Daniela
authors.initials: D
authors.lastname: Fusar-Poli
authors.forename: Marina
authors.initials: M
authors.lastname: Sironi
authors.forename: Alberto
authors.initials: A
authors.lastname: Mantovani
authors.forename: Aldo
authors.initials: A
authors.lastname: Bernelli-Zazzera
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003720:Densitometry; D006358:Hot Temperature; D015151:Immunoblotting; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D009841:Oligonucleotides / Q000737:chemistry; D018384:Oxidative Stress; D010766:Phosphorylation; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion; D050796:STAT3 Transcription Factor; D013997:Time Factors; D015534:Trans-Activators / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D015850:Interleukin-6; D009841:Oligonucleotides; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat; D015534:Trans-Activators