[Studies on the toxicity of polymer of glycolide and lactide(PLGA) and the anti-fertility effect of levonorgestrel(LNG)].
PLGA(9:1), a chemical degraded from biomaterials, has been subjected to the tests of acute toxicity, irritations on conjunctiva and muscle, haemolysis in vitro and allergic reaction of mice and rats. The anti-fertility effect of PLGA-LNG microphere composite on the same animals also has been tested. The studies demonstrated that PLGA had fine biological compatibility without side effects such as toxicity, irritations on conjunctiva and muscle, pyrogenicity, haemolysis in vitro and allergic reaction. The contraception period of mice lasted 11.5, 34.6 and 81.2 days respectively after the subcutaneous (s.c.) injection of PLGA-LNG microphere which contains LNG 5, 10 and 20 mg/kg separately. The intramuscular(i.m.) injection of PLGA-LNG microphere containing LNG 13.6, 19.8 and 30 mg/kg respectively could keep the estrus of rats supressed for 76.3, 104.1 and 131.8 days. The injections of PLGA-LNG microphere through s.c. or i.m. showed more significant effects than the same dosages of PLGA-LNG micro-crystal. These results suggest that PLGA-LNG microphere injection has long-term anti-fertility effect.
Jin
A
A
Zhejiang Academy of Medical Sciences, Hangzhou 310013.
Zhang
Y
Y
Jiao
J
J
Zhu
J
J
Zhou
Z
Z
Tao
Q
Q
D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D003270:Contraceptive Agents / Q000494:pharmacology*; D004337:Drug Carriers; D005260:Female; D006168:Guinea Pigs; D019344:Lactic Acid / Q000633:toxicity*; D016912:Levonorgestrel / Q000494:pharmacology*; D008297:Male; D008422:Materials Testing; D051379:Mice; D008863:Microspheres; D011100:Polyglycolic Acid / Q000633:toxicity*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000633:toxicity*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
D001672:Biocompatible Materials; D003270:Contraceptive Agents; D004337:Drug Carriers; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D016912:Levonorgestrel