Laser capture microdissection as an aid to ultrastructural analysis.
Laser capture microdissection uses a microscope to identify specific cells for microdissection and then a laser-sensitive plastic to capture and remove the cells from their substrate. This efficient capture method was originally developed to capture cells for genetic analysis. However, it has also been used to capture cells for proteonomic analysis. In this article, we extend the uses of laser-capture microdissection by reporting a method for preparing captured cells for ultrastructural analysis by transmission electron microscopy. Cells prepared by our methodology show good fine structure preservation and are easily sectioned by standard ultramicrotomy.
Grant
Kenneth
K
Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC 27157-1092, USA.
Jerome
W Gray
WG
D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured; D004210:Dissection / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007834:Lasers; D008247:Lysosomes / Q000648:ultrastructure; D008846:Micromanipulation / Q000379:methods*; D008854:Microscopy, Electron; D008871:Microvilli / Q000648:ultrastructure; D008928:Mitochondria / Q000648:ultrastructure; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D014617:Vacuoles / Q000648:ultrastructure