[The modulation of type I collagen and its receptor system on biological characteristics of osteoblasts].
This paper addresses the question whether type I collagen-receptor system is necessary for the functional activity of osteoblasts. Through blocking type I collagen-receptor system by type I collagen antibody or integrin alpha 2 beta 1 antibody, the proliferation of osteoblasts was studied by cell count, the apoptosis was studied by flow cytometry, and the mRNA expressions of type I collagen, integrin alpha 2 beta 1 and osteocalcin were also detected by RT-PCR techniques. When type I collagen-receptor system was blocked, osteoblasts showed high apoptosis rate, lower growth kinetics and weak mRNA expression of type I collagen, integrin alpha 2 beta 1 and osteocalcin; the blocking effect was reversible. It suggests that type I collagen-receptor system is necessary for osteoblastic phenotype. The biomaterials for bone tissue engineering should be constructed according to the extracellular matrix of osteoblasts, which supply normal extracellular environment for osteoblasts.
Yang
Z
Z
Department of Orthopedic Surgery, First Affiliated Hospital, WCUMS, Chengdu 610041.
Yu
X
X
Qu
Y
Y
Xie
H
H
D017209:Apoptosis; D002455:Cell Division; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D004622:Embryo, Mammalian; D006801:Humans; D038982:Integrin alpha2beta1 / Q000096:biosynthesis* / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D015675:Osteocalcin / Q000096:biosynthesis / Q000235:genetics; D010641:Phenotype; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D024042:Collagen Type I; D038982:Integrin alpha2beta1; D012333:RNA, Messenger; D015675:Osteocalcin