[Enantiomeric separations of 1-1,2,4-triazole compounds by micro-high performance liquid chromatography].
The enantiomeric separations of sixteen novel 1-1,2,4-triazole chiral compounds were investigated by micro-HPLC with a 200 mm x 100 microns i.d. capillary chiral OD column. The detection was performed at 223 nm. The mobile phase was a mixture of iso-propyl alcohol and n-hexane(20:80, V/V) at a flow rate of 30 microL/min and column head pressure of 6.9 MPa. Some of the enantiomers could be separated on baseline. In comparing with the separation results by the conventional HPLC method, the micro-HPLC method had the advantages of high-speed, economy, environmental friendliness etc. The effect of solute structure on enantio-selectivity was investigated and the chiral recognition mechanism was also discussed. It was found that the main separation mechanisms involve the pi-pi association and inclusion between the substituent R and the spiral pocket of OD-CSP.
Jiang
Z J
ZJ
State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, China.
Gao
R Y
RY
Zhang
K
K
Zhang
Z C
ZC
Wang
Q S
QS
Yan
C
C
D019840:2-Propanol; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006540:Herbicides / Q000032:analysis / Q000302:isolation & purification*; D006586:Hexanes; D013237:Stereoisomerism; D014227:Triazines; D014230:Triazoles / Q000032:analysis / Q000302:isolation & purification*
D006540:Herbicides; D006586:Hexanes; D014227:Triazines; D014230:Triazoles; C045575:1,2,4-triazole; C026385:n-hexane; D019840:2-Propanol