title: [Isolation and characterization of mutants of Aspergillus niger deficient in extracellular proteases].
abstract: Several mutants of Aspergillus niger 3.795, deficient in extracellular protease expression, have been isolated and characterized biochemically after in vitro UV-mutagenesis of conidiospores and selected by halo-screening on the gelatin/casein plate and skim milk plate. Extracellular proteolytic activities of 3.795-1-23 and 3.795-1-30 are 5.4% and 8.4% of the parental strain and these strains may be used as the host for efficient expression of foreign proteins.
authors.affiliation: Institute of Medicinal Biotechnology, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100050.
authors.forename: B
authors.initials: B
authors.lastname: Hong
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Li
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D016296:Mutagenesis; D014466:Ultraviolet Rays
chemical_list: D010450:Endopeptidases