[Amplification of phytoplasma 16S rDNA from banana bunchy top disease, RFLP and sequence analysis of 16S rDNA fragment].
Extract DNA from banana plants with banana bunchy top disease and amplify the phytoplasma 16S rDNA fragment by nested PCR. The classification of the phytoplasma established on the basis of 16S rDNA restriction patterns was examined by performing a sequence analysis of 16S rDNA fragment. The RFLP pattern, sequence data and phylogenic tree showed that this phytoplasma should belong to Group I(Phytoplasma asteri).
Li
H
H
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
Qiu
B
B
Li
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L
Shi
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C
D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D008969:Molecular Sequence Data; D028521:Musa / Q000382:microbiology*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D008973:Tenericutes / Q000145:classification / Q000235:genetics*
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S