The immunoassay of methotrexate by capillary electrophoresis with laser-induced fluorescence detection.
Immunoassay is widely employed as a highly sensitive, specific analytical method for hormones and drugs in biological samples. A technique utilizing capillary electrophoresis with laser-induced fluorescence detection was examined based on the reaction process of these immunoassays in order to develop a protocol characterized by high sensitivity and high speed. The conditions of the antigen-antibody reaction and capillary electrophoresis were variously examined using fluorescein-labeled methotrexate and the antibody of methotrexate. As a result, the immunoassay could be completed within a few minutes. Moreover, detection in the pg range could be accomplished. The sensitivity corresponded to that of radioimmunoassay. A simultaneous multi-component analysis of the immunoassay is also possible due to the high resolving power of capillary electrophoresis. In this study, the possibility of a simultaneous analysis of methotrexate and vancomycin was also investigated.
Suzuki
Yoshio
Y
School of Pharmaceutical Sciences, Showa University, Shinagawa, Tokyo 142-8555, Japan.
Arakawa
Hidetoshi
H
Maeda
Masako
M
D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods*; D007834:Lasers; D008727:Methotrexate / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014640:Vancomycin / Q000032:analysis
D014640:Vancomycin; D008727:Methotrexate