[Separation of two isomers in the products of condensation reaction with trimethoxybenzaldehyde as raw material and quick determination of the concentration of its intermediates by high performance liquid chromatography].
The content ratio of the two isomers in the products of condensation reaction with trimethoxybenzaldehyde as raw material is the key factor to improve the productivity of trimethoprim(TMP). A quantitative method using Nova-Pak C18 column (3.9 mm i.d. x 150 mm, 4 microns) and tetrahydrofuran(THF)-water (30:70, V/V) as mobile phase with flow rate of 1.0 mL/min and UV 320 nm detector was established. A good linearity was obtained in the concentration range of 0.01 mg.L-1-10 mg.L-1. The detectable limits of trimethoxybenzaldehyde and alpha-(3,4,5-trimethoxy-benzal)-beta-methoxy-propionitrile were 2.0 micrograms/L and 1.0 microgram/L respectively. The method has been used to analyze real samples with satisfactory results.
Qiu
D F
DF
Department of Chemistry, Nanyang Teachers College, Nanyang 473061, China. qiu_df@163.net
Wang
L
L
Wang
H W
HW
D001547:Benzaldehydes / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D009570:Nitriles / Q000032:analysis / Q000302:isolation & purification*; D013237:Stereoisomerism; D014295:Trimethoprim / Q000032:analysis / Q000302:isolation & purification*
D001547:Benzaldehydes; D009570:Nitriles; C478423:alpha-(3,4,5-trimethoxy-benzal)-beta-methoxy-propionitrile; C034031:2,4,5-trimethoxybenzaldehyde; D014295:Trimethoprim