Heat acclimation prolongs the time to central nervous system oxygen toxicity in the rat. Possible involvement of HSP72.
Oxygen toxicity of the central nervous system (CNS-OT) can occur during diving with oxygen-enriched gas mixtures, or during hyperbaric medical treatment. CNS-OT is characterised by convulsions and sudden loss of consciousness, which may be fatal in diving. Heat acclimation is known to provide cross-tolerance to various forms of stress in different organs, including the brain. We hypothesised that heat acclimation may delay the onset of CNS-OT in the rat. Male Sprague-Dawley rats were acclimated to an ambient temperature of 32 degrees C for 4 weeks. Rats in the control group were kept at 24 degrees C. Both groups were exposed to oxygen at 608 kPa. EEG was recorded continuously until the appearance of the first electrical discharge preceding clinical convulsions. CO(2) production was measured simultaneously with the EEG. Latency to CNS-OT was measured and brain samples were taken for evaluation of heat shock protein 72 (HSP72) levels by Western blot analysis at the end of the acclimation period and during 4 weeks of deacclimation. Latency to CNS-OT was twice as long in the heat-acclimated rat, with insignificant changes in CO(2) production. This prolongation continued for 2 weeks during deacclimation. There was a significant increase in the level of HSP72 following heat acclimation, with a subsequent decrease during deacclimation. We conclude that heat acclimation prolongs latency to CNS-OT in a way that does not involve changes in metabolic rate. During deacclimation there was a linear relationship between latency to CNS oxygen toxicity and the level of HSP72. A possible beneficial effect of HSP72 is discussed.
Arieli
Yehuda
Y
Israel Naval Medical Institute, IDF Medical Corps, P.O. Box 8040, Haifa 31080, Israel. yehuda_a@maoz.org.il
Eynan
Mirit
M
Gancz
Hanan
H
Arieli
Ran
R
Kashi
Yechezkel
Y
D000064:Acclimatization / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects*; D002245:Carbon Dioxide / Q000032:analysis; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006358:Hot Temperature; D008297:Male; D010100:Oxygen / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D002245:Carbon Dioxide; D010100:Oxygen