[High level expression of human recombinant albumin gene in the yeast Pichia pastoris].
The yeast Pichia pastoris was transformed by the multi-copy Pichia expression vector that can express secreted human albumin. The high level expression of cell line was selected after screening. The expression of human recombinant albumin in Pichia pastoris induced by different methods were compared. The ratio of secreted human albumin is 80% in total secreted proteins and the expression level reaches as high as is 10 g/L.
Cui
Y
Y
Research Center of Molecular Medicine, Sun Yat-Sen University of Medical Sciences, Guangzhou 510089, China.
Ming
W
W
Yin
W
W
Ren
Z
Z
Wang
J
J
Gu
J
J
D000418:Albumins / Q000096:biosynthesis* / Q000235:genetics; D005285:Fermentation; D005990:Glycerol / Q000494:pharmacology; D006801:Humans; D000432:Methanol / Q000494:pharmacology; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics
D000418:Albumins; D011994:Recombinant Proteins; D005990:Glycerol; D000432:Methanol