title: [Effects of fluid shear stress on PGE2 synthesis of primary osteoblast-like cells in rats].
abstract: OBJECTIVE
  The purpose of this study is to study the function of PGE2 during the signal transduction in which fluid shear stress regulates the proliferation and differentiation of osteoblast-like cells.
  
  
  METHODS
  The isolated rat primary osteoblast-like cells were exposed to fluid shear stress 0.12 mN/cm2 for 5, 10, 15, 30, 60 and 120 minutes respectively in a flow chamber. The release of PGE2 was examined.
  
  
  RESULTS
  After exposure to fluid shear stress, the PGE2 synthesis of rat primary osteoblast-like cells increased significantly (P < 0.01) when compared with the control. After 10 minutes, the release of PGE2 began to increase significantly (P < 0.01) and the effect was maximal after 60 minutes (P < 0.01).
  
  
  CONCLUSIONS
  PGE2 pathway may be one of the signal-transduction pathways which can transduce the fluid shear stress into osteoblast-like cells and then stimulate the bone remodeling.
authors.affiliation: College of Stomatology, West China University of Medical Sciences.
authors.forename: J
authors.initials: J
authors.lastname: Qiao
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Luo
authors.forename: W
authors.initials: W
authors.lastname: Tian
authors.forename: M
authors.initials: M
authors.lastname: Zhang
authors.forename: Q
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D016723:Bone Remodeling; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis*; D010006:Osteoblasts / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012212:Rheology; D033081:Shear Strength; D015398:Signal Transduction / Q000502:physiology
chemical_list: D015232:Dinoprostone