Immobilization of D-ribulose-1,5-bisphosphate carboxylase/oxygenase: a step toward carbon dioxide fixation bioprocess.
Immobilization of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from spinach leaves is described. This enzyme enables the fixation of carbon dioxide on a five-carbon sugar D-ribulose-1,5-bisphosphate (RuBP). Two different immobilization methods were employed: dicyclohexylcarbodiimide coupling on nylon membrane matrix and dimethylpimelimidate immobilization on protein A agarose. The reusability of immobilized enzymes, coupling efficiency, and temperature-activity relationship of soluble and immobilized Rubisco are presented. The immobilization imparted greater thermal and storage stability. The thermal deactivation rates of the immobilized enzymes were considerably lower than those of the soluble enzyme.
Chakrabarti
Subhra
S
ABRD Company LLC, 1555 Wood Road, Cleveland, Ohio 44121, USA.
Bhattacharya
Sumana
S
Bhattacharya
Sanjoy K
SK
D002245:Carbon Dioxide / Q000737:chemistry; D004024:Dicyclohexylcarbodiimide / Q000737:chemistry*; D004356:Drug Storage / Q000379:methods; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007096:Imidoesters / Q000737:chemistry*; D008567:Membranes, Artificial; D009757:Nylons; D018515:Plant Leaves / Q000737:chemistry / Q000201:enzymology; D012273:Ribulose-Bisphosphate Carboxylase / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D012685:Sepharose / Q000031:analogs & derivatives* / Q000737:chemistry*; D018724:Spinacia oleracea / Q000737:chemistry / Q000201:enzymology; D013696:Temperature; D013205:Staphylococcal Protein A
D002245:Carbon Dioxide; D004024:Dicyclohexylcarbodiimide; D004800:Enzymes, Immobilized; D007096:Imidoesters; D008567:Membranes, Artificial; D009757:Nylons; D012273:Ribulose-Bisphosphate Carboxylase; D012685:Sepharose; C042030:Staphylococcal protein A-sepharose; D013205:Staphylococcal Protein A; C029326:dimethyl pimelimidate