Crystallization of membrane proteins from media composed of connected-bilayer gels.
The use of hydrated-lipid gels in which the bilayer is an infinitely periodic (or at least continuous), three-dimensional structure offers a relatively new approach for the crystallization of membrane proteins. While excellent crystals of the Halobacterial rhodopsins have been obtained with such media, success remains poor in extending their use to other membrane proteins. Experience with crystallization of bacteriorhodopsin has led us to recognize a number of improvements that can be made in the use of such hydrated-gel media, which may now prove to be of general value for the crystallization of other membrane proteins.
Rouhani
Shahab
S
Life Sciences Division, Donner Laboratory, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA.
Facciotti
Marc T
MT
Woodcock
Glenna
G
Cheung
Vincent
V
Cunningham
Christian
C
Nguyen
Doris
D
Rad
Behzad
B
Lin
Chiann-Tso
CT
Lunde
Christopher S
CS
Glaeser
Robert M
RM
D001436:Bacteriorhodopsins / Q000737:chemistry / Q000648:ultrastructure; D003460:Crystallization; D018360:Crystallography, X-Ray; D003470:Culture Media; D005782:Gels; D006217:Halobacterium; D008051:Lipid Bilayers / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D008958:Models, Molecular; D008968:Molecular Conformation; D011487:Protein Conformation; D013816:Thermodynamics
D003470:Culture Media; D005782:Gels; D008051:Lipid Bilayers; D008563:Membrane Lipids; D008565:Membrane Proteins; D001436:Bacteriorhodopsins