A Gag-Pol/Env-Rev SIV239 DNA vaccine improves CD4 counts, and reduce viral loads after pathogenic intrarectal SIV(mac)251 challenge in rhesus Macaques.
DNA vaccines are an important vaccine approach for many infectious diseases including human immunodeficiency virus (HIV). Recently, there have been exciting results reported for plasmid vaccination in pathogenic SHIV model systems. In these studies, plasmid vaccines supplemented by IL-2 Ig cytokine gene adjuvants or boosted by recombinant MVA vectors expressing relevant SIV and HIV antigens prevented CD4(+) T-cell loss and lowered viral loads following pathogenic challenge. However, similar results have not been reported in a direct pathogenic macaque challenge model. Here we report on a study of the ability of a multiplasmid SIV DNA vaccine in a pathogenic SIV251 rhesus mucosal challenge study. We observed that pGag/Pol+pEnv/Rev plasmid vaccines could not prevent SIV infection; however, vaccinated animals exhibited significant improvement in control of viral challenge compared to control animals. Furthermore, vaccinated animals exhibited protection against CD4(+) T-cell loss.
Muthumani
Karuppiah
K
Department of Pathology and Laboratory of Medicine, University of Pennsylvania, 505 Stellar Chance Laboratories, 422 Curie Blvd, Philadelphia, PA 19104, USA.
Bagarazzi
Mark
M
Conway
Dan
D
Hwang
Daniel S
DS
Manson
Kelledy
K
Ciccarelli
Richard
R
Israel
Zimmra
Z
Montefiori
David C
DC
Ugen
Kenneth
K
Miller
Nancy
N
Kim
Jong
J
Boyer
Jean
J
Weiner
David B
DB
D000818:Animals; D018791:CD4 Lymphocyte Count; D015685:Fusion Proteins, gag-pol / Q000235:genetics / Q000276:immunology; D015686:Gene Products, env / Q000235:genetics / Q000276:immunology; D015694:Gene Products, rev / Q000235:genetics / Q000276:immunology; D008253:Macaca mulatta; D018449:SAIDS Vaccines / Q000276:immunology; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology; D019562:Viral Load
D015685:Fusion Proteins, gag-pol; D015686:Gene Products, env; D015694:Gene Products, rev; D018449:SAIDS Vaccines; D019444:Vaccines, DNA