title: Isolation and characterization of four cell wall purple acid phosphatase genes from tobacco cells.
abstract: Four full-length cDNAs were isolated from a cDNA library prepared from tobacco cultured cells and designated NtPAP4, NtPAP12, NtPAP19 and NtPAP21, which could correspond to purple acid phosphatase (PAP). Levels of both NtPAP12 and NtPAP21 mRNA in the protoplasts immediately increased after the protoplasts were transferred to a medium for cell wall regeneration, and the accumulation of the mRNA was correlated with cell wall regeneration for 3 h. It is likely that the NtPAP12 and NtPAP21 gene products are wall-bound PAPs at the early stage of regenerating walls in tobacco protoplasts.
authors.affiliation: Division of Material and Biological Sciences, Graduate School of Science, Japan Women's University, 2-8-1 Mejirodai, Bunkyo, Tokyo 112-8681, Japan. rumi.kadda00@gr.jwu.ac.jp
authors.forename: Rumi
authors.initials: R
authors.lastname: Kaida
authors.forename: Kimiyo
authors.initials: K
authors.lastname: Sage-Ono
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kamada
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Okuyama
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Syono
authors.forename: Takako S
authors.initials: TS
authors.lastname: Kaneko
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D002473:Cell Wall / Q000201:enzymology*; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000302:isolation & purification; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D010802:Phylogeny; D011523:Protoplasts / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment; D013997:Time Factors; D014026:Nicotiana / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D006023:Glycoproteins; D007527:Isoenzymes; D010446:Peptide Fragments; D012333:RNA, Messenger; C091137:purple acid phosphatase; D000135:Acid Phosphatase