title: Crystallization and preliminary X-ray crystallographic analysis of Sclerotium rolfsii lectin.
abstract: Sclerotium rolfsii lectin (SRL), from the soil-borne phytopathogenic fungus S. rolfsii, has been crystallized. SRL crystals were grown by the hanging-drop vapour-diffusion method using an MPD-ammonium acetate mixture in Tris-HCl buffer pH 8.5. A complete data set from a single crystal at 100 K was collected to 1.1 A resolution using synchrotron radiation. Preliminary crystallographic analysis showed that the crystals belong to the tetragonal space group P4(2)2(1)2, with unit-cell parameters a = b = 99.81, c = 63.99 A and two molecules per asymmetric unit.
authors.affiliation: Institute of Biological Research and Biotechnology, The National Hellenic Research Foundation, 48 Vas. Constantinou Ave, 11635 Athens, Greece. ddl@eie.gr
authors.forename: Demetres D
authors.initials: DD
authors.lastname: Leonidas
authors.forename: Bale M
authors.initials: BM
authors.lastname: Swamy
authors.forename: Anuradha G
authors.initials: AG
authors.lastname: Bhat
authors.forename: Shashikala R
authors.initials: SR
authors.lastname: Inamdar
authors.forename: Magda N
authors.initials: MN
authors.lastname: Kosmopoulou
authors.forename: Evangelia D
authors.initials: ED
authors.lastname: Chrysina
authors.forename: Nikos G
authors.initials: NG
authors.lastname: Oikonomakos
mesh_terms: D001203:Ascomycota / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*
chemical_list: D005656:Fungal Proteins; D037102:Lectins