[A study on ovariectomized goat osteoblast culture in vitro].
Osteoblasts were isolated from the frontal parietal bone of ovariectomized goat and were confirmed by testing for multiple biological features. The results showed that the goat osteoblasts cultured had the typical morphological feature of osteoblasts, the activity of alkaline phosphatase, and the ability of calcification in vitro. Also, the results proved that these goat osteoblasts in vitro had the same function and features as those in vivo. The experimental techniques in this study have provided a useful system for researches in bone metabolism.
Deng
L
L
College of Pharmacy, West China University of Medical Sciences, Chengdu 610041.
Chen
H
H
Zheng
H
H
Peng
X
X
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D005260:Female; D006041:Goats; D010006:Osteoblasts / Q000000981:diagnostic imaging / Q000201:enzymology / Q000502:physiology; D010052:Ovariectomy; D014463:Ultrasonography
D000469:Alkaline Phosphatase