The role of nuclear factor-kappaB essential modulator (NEMO) in B cell development and survival.
The transcription factor nuclear factor-kappaB (NF-kappaB) is essential for immune and inflammatory responses. NF-kappaB essential modulator (NEMO) is a scaffolding component of the IkappaB kinase complex required for NF-kappaB activation in vitro. Because NF-kappaB activation is involved in B cell development and function, we set out to determine whether NEMO is required for these processes. NEMO(-/-) mice die very early during embryogenesis, and fetal livers from NEMO(-/-) embryos can not reconstitute either B or T lymphopoiesis in irradiated host mice. We therefore used NEMO(-/-) embryonic stem cells and the OP9 in vitro differentiation system to demonstrate that NEMO is not required for B cell development but plays an important role in B cell survival.
Kim
Shinyop
S
Advanced Medical Discovery Institute, Ontario Cancer Institute and Department of Medical Biophysics and Immunology, University of Toronto, 620 University Avenue, Toronto, ON, Canada M5G 2C1.
La Motte-Mohs
Ross N A
RN
Rudolph
Dorothea
D
Zuniga-Pflucker
Juan Carlos
JC
Mak
Tak W
TW
D000818:Animals; D018941:Antigens, CD19 / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D018122:B7-1 Antigen / Q000096:biosynthesis; D002454:Cell Differentiation; D002460:Cell Line; D002469:Cell Separation; D002470:Cell Survival; D018920:Coculture Techniques; D004622:Embryo, Mammalian / Q000166:cytology; D005434:Flow Cytometry; D051550:I-kappa B Kinase; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D017346:Protein Serine-Threonine Kinases / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology; D013997:Time Factors
D018941:Antigens, CD19; D018122:B7-1 Antigen; D007075:Immunoglobulin M; D017346:Protein Serine-Threonine Kinases; C496564:Chuk protein, mouse; D051550:I-kappa B Kinase; C496562:Ikbkb protein, mouse; C496566:Ikbke protein, mouse