An alternatively spliced variant of cathepsin E in human gastric adenocarcinoma cells.
Splice variant and authentic mRNAs for procathepsin E were measured at a ratio of 5:1 and 1:2 in Kato 3 and AGS cells, two human gastric adenocarcinoma cell lines. As a result of the precise splicing of the 3'-end of exon 6 to the 5'-end of exon 8, the variant lacked the 142 bp of exon 7 which encodes the second of the Asp residues that operate the catalytic mechanism of aspartic proteinases.
Tatnell
Peter J
PJ
School of Biosciences of Cardiff University, Museum Avenue, P.O. Box 911, Cardiff CF10 3US, Wales, UK.
Cook
Matthew
M
Kay
John
J
D000230:Adenocarcinoma / Q000378:metabolism*; D017398:Alternative Splicing; D020059:Cathepsin E / Q000235:genetics* / Q000378:metabolism; D002403:Cathepsins / Q000235:genetics* / Q000378:metabolism; D004792:Enzyme Precursors / Q000235:genetics* / Q000378:metabolism; D005091:Exons; D014644:Genetic Variation; D006801:Humans; D007438:Introns; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification; D013274:Stomach Neoplasms / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D004792:Enzyme Precursors; D012333:RNA, Messenger; D002403:Cathepsins; C067581:procathepsin E; D020059:Cathepsin E