The adjuvant OM-174 induces both the migration and maturation of murine dendritic cells in vivo.
The aim of this study was to test the capacity of the novel adjuvant OM-174, a lipid A analog, to induce the migration and the maturation of murine dendritic cells (DC) in vivo, a step which is considered as the initiation of the adaptive immune response. BALB/c mice were injected intravenously or subcutaneously with OM-174. The spleen and popliteal lymph nodes were harvested, and analyzed for DC localization and phenotype. The data presented here clearly show that, OM-174 induces the migration of DC from the periphery to the T cell areas of lymphoid organs, and their maturation into cells expressing high levels of MHC class II and co-stimulatory molecules, with a potency close to that of Escherichia coli lipopolysaccharide (LPS).
Pajak
Bernard
B
Laboratory of Animal Physiology, Université Libre de Bruxelles, 6041, Gosselies, Belgium.
Garze
Virginie
V
Davies
Gwyn
G
Bauer
Jacques
J
Moser
Muriel
M
Chiavaroli
Carlo
C
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000938:Antigen-Presenting Cells / Q000145:classification / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003713:Dendritic Cells / Q000145:classification / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology; D005260:Female; D007275:Injections, Intravenous; D007279:Injections, Subcutaneous; D007801:Langerhans Cells / Q000145:classification / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology
D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; C400105:defoslimod