Hydrogen-potassium ATPase inhibitors induce relaxation on rabbit prostatic strips in vitro.
BACKGROUND
To determine the relaxant effect of omeprazole and lansaprazole, hydrogen-potassium (H+-K+) ATPase inhibitors, on rabbit prostatic tissue in vitro.


METHODS
Male New Zealand white rabbits were sacrificed and their prostatic tissues were removed. The prostatic stromal strips were mounted in organ baths and relaxation responses were obtained in precontracted tissues with phenylephrine, carbachol and potassium chloride (KCl). Relaxation responses were controlled in the presence of various antagonists to explain the mechanism for relaxation exerted by omeprazole and lansaprazole.


RESULTS
Omeprazole and lansaprazole caused similar relaxation responses in the prostatic strips precontracted with phenylephrine, carbachol and KCl. The addition of prostaglandin synthase inhibitor indomethacin, nitric oxide synthase inhibitor L-NAME, potassium channel blockers, glibenclamide and tetraethylammonium into the organ baths did not change the relaxations induced by omeprazole and lansaprazole in vitro.


CONCLUSIONS
Omeprazole and lansaprazole cause a relaxation in prostatic stromal tissue precontracted with phenyephrine, carbachol and KC1 in vitro. This relaxant effect is independent of H+-K+ ATPase inhibition. Additionally, cyclooxygenase and nitric oxide pathways do not contribute to this relaxant effect. Further studies are required to determine whether these drugs may have a beneficial effect in the non-operative treatment of benign prostatic hyperplasia.
Bagcivan
Ihsan
I
Department of Pharmacology, Cumhuriyet University, School of Medicine, Sivas, Turkey.
Gökçe
Gökhan
G
Ayan
Semih
S
Yildirim
Sahin
S
Saraç
Bülent
B
Kiliçarslan
Hakan
H
Sarioglu
Yusuf
Y
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000818:Animals; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D017506:H(+)-K(+)-Exchanging ATPase / Q000494:pharmacology* / Q000627:therapeutic use; D066298:In Vitro Techniques; D064747:Lansoprazole; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects*; D009129:Muscle Tonus / Q000187:drug effects; D009853:Omeprazole / Q000031:analogs & derivatives / Q000494:pharmacology* / Q000627:therapeutic use; D011467:Prostate / Q000187:drug effects*; D011470:Prostatic Hyperplasia / Q000188:drug therapy*; D054328:Proton Pump Inhibitors; D011817:Rabbits; D017154:Stromal Cells / Q000187:drug effects
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D004791:Enzyme Inhibitors; D054328:Proton Pump Inhibitors; D064747:Lansoprazole; D017506:H(+)-K(+)-Exchanging ATPase; D009853:Omeprazole