Effects of colony-stimulating factors on the secretion of platelet-activating factor acetylhydrolase by human decidual macrophages.
OBJECTIVE
The aim is to clarify the role of platelet-activating factor and colony-stimulating factors in term and preterm parturition.


METHODS
Decidual macrophage populations were obtained by an enzymic digestion, Ficoll-Paque centrifugation, or flow cytometric sorting. The effects of colony-stimulating factors on the platelet-activating factor acetylhydrolase secretion by these cells were examined.


RESULTS
Granulocyte-macrophage colony-stimulating factor inhibited the platelet-activating factor acetylhydrolase secretion by decidual macrophages. Granulocyte colony-stimulating factor also decreased the enzyme secretion but at higher concentrations than those required for granulocyte colony-stimulating factor. In contrast, macrophage colony-stimulating factor increased the enzyme secretion. These colony-stimulating factor-induced effects were specifically neutralized by the corresponding antibodies.


CONCLUSIONS
Colony-stimulating factors may modulate the local concentration of platelet-activating factor in the decidua via their inhibitory or stimulatory effect on the secretion of platelet-activating factor acetylhydrolase, contributing to the regulation of term and preterm parturition at the maternal-fetal interface.
Narahara
Hisashi
H
Department of Obstetrics and Gynecology, Oita Medical University, Oita, Japan.
Mine
Shin-Ichiro
S
Kawano
Yasushi
Y
Johnston
John M
JM
Miyakawa
Isao
I
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002469:Cell Separation; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D017364:Collagenases / Q000378:metabolism; D003115:Colony-Stimulating Factors / Q000494:pharmacology* / Q000502:physiology*; D003656:Decidua / Q000166:cytology* / Q000201:enzymology; D003851:Deoxyribonucleases / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor / Q000276:immunology / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000494:pharmacology; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000494:pharmacology; D008264:Macrophages / Q000201:enzymology*; D010741:Phospholipases A / Q000378:metabolism*; D011247:Pregnancy; D011994:Recombinant Proteins / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D003115:Colony-Stimulating Factors; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D003851:Deoxyribonucleases; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D006821:Hyaluronoglucosaminidase; D017364:Collagenases