Protective effects of xanthones against myocardial ischemia-reperfusion injury in rats.
OBJECTIVE
To investigate the protective effect of xanthones against myocardial ischemia-reperfusion injury in rats.


METHODS
Ischemia-reperfusion injury was induced by 20 min of global ischemia and 40 min of reperfusion in isolated rat hearts or 60-min coronary artery occlusion and 180-min reperfusion in vivo, respectively. Heart rate, coronary flow, left ventricular pressure (LVP), and its first derivative (+/- dp/dtmax) were recorded, and the activity of creatine kinase in coronary effluent and malondialdehyde contents in myocardial tissues were measured in vitro. The activity of serum creatine kinase and myocardium infarct size were measured in vivo.


RESULTS
Xanthones (90 or 300 microg/L) caused a significant improvement of cardiac function (LVP and +/- dp/dtmax) and a decrease in the release of creatine kinase in coronary effluent as well as the level of malondialdehyde in myocardial tissues. Xanthones (0.5 or 1.0 mg/kg) also markedly decreased infarct size and the release of creatine kinase in vivo.


CONCLUSIONS
Xanthones protect the myocardium against the damages induced by ischemia-reperfusion in rats, and the effect of xanthones may be related to the inhibition of lipid peroxidation.
Jiang
De-Jian
DJ
Department of Pharmacology, School of Pharmaceutic Sciences, Central South University, Changsha 410078, China.
Tan
Gui-Shan
GS
Ye
Feng
F
Du
Yan-Hua
YH
Xu
Kang-Ping
KP
Li
Yuan-Jian
YJ
D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D003326:Coronary Circulation / Q000187:drug effects; D003402:Creatine Kinase / Q000097:blood; D006321:Heart / Q000187:drug effects / Q000503:physiopathology; D006339:Heart Rate / Q000187:drug effects; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D017202:Myocardial Ischemia; D015428:Myocardial Reperfusion Injury / Q000201:enzymology / Q000378:metabolism / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D029967:Swertia / Q000737:chemistry; D017725:Ventricular Pressure / Q000187:drug effects; D044004:Xanthones / Q000302:isolation & purification / Q000494:pharmacology*
D002316:Cardiotonic Agents; D044004:Xanthones; D008315:Malondialdehyde; D003402:Creatine Kinase