A potent, water-soluble and photoinducible DNA cross-linking agent.
Water-soluble DNA cross-linking phenol and biphenol derivatives 3 and 6 have been synthesized by a Mannich reaction. Both of them can cross-link DNA by photoactivation using visible light (wavelength > 400 nm). Compound 6 can cross-link DNA at pH 5.0 and 7.7, whereas no cross-link was observed at pH 10.0. Density functional theory (DFT) calculation indicated that 6 displays a twist structure. Therefore, it could bind to DNA naturally and has potent property to cross-link DNA after photoactivation.
Wang
Ping
P
College of Chemistry and Molecular Sciences, Wuhan University, Hubei Wuhan 430072, P. R. of China.
Liu
Renpeng
R
Wu
Xiaojun
X
Ma
Hongjuan
H
Cao
Xiaoping
X
Zhou
Ping
P
Zhang
Jiangye
J
Weng
Xiaocheng
X
Zhang
Xiao-Lian
XL
Qi
Jun
J
Zhou
Xiang
X
Weng
Linhong
L
D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D010636:Phenols / Q000138:chemical synthesis / Q000737:chemistry*; D010777:Photochemistry; D000644:Quaternary Ammonium Compounds / Q000138:chemical synthesis / Q000737:chemistry; D012995:Solubility; D014867:Water / Q000737:chemistry
D003432:Cross-Linking Reagents; D010636:Phenols; D000644:Quaternary Ammonium Compounds; D014867:Water; D004247:DNA