[The influence of somatostatin 14 on the proliferation of human retroocular fibroblasts].
OBJECTIVE
To elucidate the possible mechanism of Somatostatin 14 in the treatment of Graves' ophthalmopathy, thus providing laboratory data with the intent to underpin therapeutic developments.


METHODS
The human retro-ocular fibroblasts (HROFb) was cultured and MTT colorimetry assay was used to determine the proliferation of HROFb.


RESULTS
MTT colorimetry assay could precisely reflect the proliferation of HROFb. The absorbance was directly proportional to the numbers of the cells (r = 0.996, P < 0.001). Somatostatin 14 could enhance the cell proliferation percentage at lower levels (< 25 nmol/L) and inhibit that at higher levels (> 25 nmol/L). The cell proliferation correlated positively to Somatostatin 14 levels significantly (r = -0.9054, P < 0.001).


CONCLUSIONS
Somatostatin 14 has a dual effect on HROFb.
An
Z
Z
Division of Endorcrinology, First Affiliated Hospital, WCUMS, Chengdu 610041, China.
Wei
S
S
Xu
S
S
Yang
H
H
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003124:Colorimetry / Q000379:methods; D005123:Eye / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology*; D006111:Graves Disease / Q000473:pathology; D006801:Humans; D013004:Somatostatin / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles
D013778:Tetrazolium Salts; D013844:Thiazoles; D013004:Somatostatin; C022616:thiazolyl blue