[Construction of a GFP broad-host-range promoter-probe vector and its application].
The 0.9 kb DNA fragment was obtained by PCR and using a pair of special designed primers from the green fluorescent protein gene(gfp). It Was then cloned into an expression vector pET-11C and named as pHN115. A promoferless gfp vector with SD fragment was obtained from pHN115 and subcloned into SK(+), pIJ 2925 and finally into pTR102. A broad-hast-range and visible promoter-probe vector, pHN127 was constructed and used successfully for the identification of constitutive and inducible promoters from Sinorhizobium fredii HN01.
Ma
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Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070.
Shi
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Q
Zhou
J
J
Chen
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H
D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020579:Sinorhizobium / Q000235:genetics*
D004269:DNA, Bacterial; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins