Expression, crystallization and activities of the two family 11 aldo-keto reductases from Bacillus subtilis.
Two members of the aldo-keto reductase family 11 from Bacillus subtilis have been crystallized and their oxidoreductase activity confirmed. AKR11A is a protein induced by inositol and repressed by glucose. AKR11B is induced when the cell is stressed by heat, acid, ethanol, starvation or osmotic shock and is therefore classified as a general stress protein. The expected NADPH-dependent sugar reductase activities for both proteins have been confirmed kinetically with several substrates. AKR11B exhibited typical aldo-keto reductase kinetics. However, only trace activity was found in AKR11A. To examine the effects of differences in sequence on the structures and functions of these enzymes, a crystallographic study has been initiated. AKR11A has been crystallized in its apo form and AKR11B crystals were obtained in complex with NADP(+).
Ehrensberger
Andreas
A
Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA.
Wilson
David K
DK
D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001412:Bacillus subtilis / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D005947:Glucose / Q000494:pharmacology; D005985:Glyceraldehyde / Q000378:metabolism; D007294:Inositol / Q000494:pharmacology; D007700:Kinetics; D009249:NADP / Q000737:chemistry / Q000378:metabolism
D005985:Glyceraldehyde; D007294:Inositol; D009249:NADP; D000429:Alcohol Oxidoreductases; D005947:Glucose