[Detection of HPV-DNA and humoral immunity in juvenile laryngeal papilloma].
OBJECTIVE
To study HPV infection route and pathogenesis of juvenile laryngeal papilloma(JLP).


METHODS
HPV-DNA of JLP was detected with PCR and PCR product dot blot hybridization. Serum Ig and complement 3(C3) was detected with nephelometry.


RESULTS
HPV total positive rate in JLP was 95%(19/20). HPV6 was 55%(11/20). HPV11 was 30%(6/20), and HPV6 + 11 was 10%(2/20). Serum IgG, IgA, IgM and C3 of JLP were normal, no significant difference between JLP and the control group(P > 0.05).


CONCLUSIONS
HPV6 infection of JLP was in the majority. And the type was consistent with the type of female genital organ pointed condyloma. Humoral immunity was negligible in HPV infection. Pathologic picture of different HPV type infection was identical.
Chen
B B
BB
Department of Otolaryngology, Second Affiliated Hospital of Wenzhou College of Medical Science, Wenzhou 325027.
Bao
Q Y
QY
Wang
Z M
ZM
Jin
J X
JX
Huang
S Y
SY
Chen
Y Y
YY
D002648:Child; D002675:Child, Preschool; D003176:Complement C3 / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000097:blood; D007223:Infant; D007822:Laryngeal Neoplasms / Q000276:immunology / Q000821:virology*; D008297:Male; D010212:Papilloma / Q000276:immunology / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections; D014412:Tumor Virus Infections
D003176:Complement C3; D004279:DNA, Viral; D007136:Immunoglobulins