[Sequencing of a beta-amylase gene from Bacillus firmus].
The gene encoding a beta-amylase from Bacillus firmus 725 was sequenced. The sequenced DNA of 2012 bp contains one open reading frame of 1406 nucleotides without a translation stop codon. The deduced amino acid sequence homology with those known bacterial and some plant beta-amylase was 98% for Bacillus polymyxa 72, 98% for Bacillus polymyxa ATCC8523, 82% for Bacillus circulans, 54% for Clostridium thermosulfurogenes, 49% for Bacillus cereus BQ10-S1, 50% for Bacillus cereus var. mycoides, 36% for barley, and 36% for soybean Eleven well-conserved regions were found among the amino acid sequences of the nine beta-amylases.
Chen
W
W
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
He
B
B
Lou
X
X
Guan
F
F
Ye
C
C
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D001614:beta-Amylase / Q000737:chemistry* / Q000235:genetics*
D001614:beta-Amylase