Targeted retroviral infection of tumor cells by receptor cooperation.
Retroviruses expressing two different receptor-binding domains linked by proline-rich spacers infect only cells expressing both retroviral receptors (Valsesia-Wittman et al., EMBO J. 6:1214-1223, 1997). Here we apply this receptor cooperation strategy to target human tumor cells by linking single-chain antibodies recognizing tumor antigens via proline-rich spacers to the 4070A murine leukemia virus surface protein.
Martin
Francisco
F
Department of Immunology and Molecular Pathology, Windeyer Institute, 46 Cleveland Street, London W1T 2AH, United Kingdom.
Chowdhury
Simon
S
Neil
Stuart J
SJ
Chester
Kerry A
KA
Cosset
Francois-Loic
FL
Collins
Mary K
MK
D000912:Antibodies, Neoplasm / Q000378:metabolism*; D002272:Carcinoembryonic Antigen / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D007128:Immunoglobulin Fragments / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism; D008979:Moloney murine leukemia virus / Q000378:metabolism / Q000472:pathogenicity*; D011991:Receptors, Virus / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000912:Antibodies, Neoplasm; D002272:Carcinoembryonic Antigen; D007128:Immunoglobulin Fragments; D008562:Membrane Glycoproteins; D011991:Receptors, Virus; C070506:ecotropic murine leukemia virus receptor