Dietary long-chain PUFA in the form of TAG or phospholipids influence lymph lipoprotein size and composition in piglets.
Several sources of long-chain PUFA (LCP) are currently available for infant formula supplementation. These oils differ in their FA composition, the chemical form of the FA esters [TAG or phospholipids (PL)], and presence of other lipid components. These differences may affect LCP absorption, distribution, and metabolic fate after ingestion. The purpose of this study was to evaluate the influence of different chemical forms of dietary LCP on the composition of lymph lipoproteins. Eighteen pigs (5 d old) were bottle-fed different diets for 2 wk: a control diet (C), a diet containing LCP as TAG from tuna and fungal oils (TF-TAG), or a diet containing LCP as PL from egg yolk (E-PL). We measured lipid and FA composition of lymph, main lymph fractions (TAG or PL), and the particle size of lymph lipoproteins. The average diameter of lymph lipoproteins was significantly lower in the E-PL group compared with the control and TF-TAG groups (C: 3902 +/- 384 A; TF-TAG: 3773 +/- 384 A; E-PL: 2370 +/- 185 A). Arachidonic acid and DHA contents in lymph and lymph-TAG were significantly higher in the TF-TAG group compared to the E-PL group (0.50 +/- 0.03 and 0.24 +/- 0.03 g/100 g vs. 0.29 +/- 0.04 and 0.12 +/- 0.03 g/100 g, respectively). The addition to the diet of LCP in the form of TAG or PL affected the size of intestinal lipoproteins and also led to a different distribution of these FA in lymph lipoproteins.
Amate
Laura
L
Research and Development Department, Abbott Laboratories, 18004 Granada, Spain.
Gil
Angel
A
Ramírez
María
M
D000818:Animals; D000831:Animals, Newborn; D004032:Diet; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000494:pharmacology; D008074:Lipoproteins / Q000737:chemistry*; D008196:Lymph / Q000737:chemistry* / Q000187:drug effects*; D008854:Microscopy, Electron; D009942:Organometallic Compounds; D010316:Particle Size; D010743:Phospholipids / Q000494:pharmacology*; D013552:Swine; D014280:Triglycerides / Q000494:pharmacology*
D004042:Dietary Fats, Unsaturated; D005231:Fatty Acids, Unsaturated; D008074:Lipoproteins; D009942:Organometallic Compounds; D010743:Phospholipids; D014280:Triglycerides; C005460:uranyl acetate