[Study of chromosome pellicle by indirect immunoflourescence method and antigen analysis in Vicia faba].
The autoimmune antiserum specific to pellicle of human metaphasic chromosomes from a lupus patient was used to stain metaphasic chromosome Vicia faba by means of indirect immunoflourescence method. It was found that the pellicles of vicia metaphasic chromosomes was positively stained. The antigen of Vicia faba recognized by the antiserum was also examined by PAGE of total cell lysate and western blotting.
Xu
T
T
Shanghai Institute of Cell Biology, Chinese Academy of Science, Shanghai 200031.
Wu
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WQ
Xu
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D000941:Antigens / Q000032:analysis*; D015153:Blotting, Western; D002877:Chromosomes, Human / Q000737:chemistry* / Q000276:immunology; D015200:Fluoroimmunoassay; D006801:Humans; D007106:Immune Sera; D008177:Lupus Vulgaris / Q000276:immunology*; D010940:Plant Proteins / Q000032:analysis*; D031307:Vicia faba / Q000737:chemistry*
D000941:Antigens; D007106:Immune Sera; D010940:Plant Proteins