In vitro immunomodulatory effects of cuphiin D1 on human mononuclear cells.
Cuphiin D1 (CD1), a macrocyclic hydrolyzable tannin isolated from Cuphea hyssopifolia, has been shown to exert antitumor activity both in vitro and in vivo. Moreover, the antitumor effects of CD1 are not only related to its cytotoxicity to carcinoma cell lines, but also depend on host-mediated mechanisms. In the present study, CD1 was investigated for its effects on the proliferation and cytokine secretion of human peripheral blood mononuclear cells (PBMCs). At concentrations of from 6.25 to 50 micrograms/ml, it enhanced the 3H-thymidine incorporation of concanavalin A (Con A)-stimulated PBMCs in a dose-dependent manner. Excretion of IL-1 beta, IL-2 and TNF-alpha by CD1-stimulated PBMCs was markedly increased in a dose-dependent manner. The results show that CD1 could stimulate PBMCs release of IL-1 beta, IL-2 and TNF-alpha and then activate T cells. Therefore, CD1-activated T cells via IL-1 beta in vitro might account for the host-mediated CD1 mechanism of action.
Wang
Ching-Chiung
CC
Graduate Institute of Pharmacognosy Science, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan, R.O.C.
Chen
Lih-Geeng
LG
Yang
Ling-Ling
LL
D000276:Adjuvants, Immunologic / Q000494:pharmacology* / Q000633:toxicity; D003208:Concanavalin A / Q000494:pharmacology; D031562:Cuphea / Q000737:chemistry; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D047348:Hydrolyzable Tannins; D007375:Interleukin-1 / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D007376:Interleukin-2 / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D013634:Tannins / Q000494:pharmacology* / Q000633:toxicity; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism
D000276:Adjuvants, Immunologic; D047348:Hydrolyzable Tannins; D007375:Interleukin-1; D007376:Interleukin-2; D013634:Tannins; D014409:Tumor Necrosis Factor-alpha; C120622:cuphiin D(1); D003208:Concanavalin A