title: A cell cycle-regulated GATA factor promotes centromeric localization of CENP-A in fission yeast.
abstract: CENP-A, the centromere-specific histone H3 variant, plays a crucial role in organizing kinetochore chromatin for precise chromosome segregation. We have isolated Ams2, a Daxx-like motif-containing GATA factor, and histone H4, as multicopy suppressors of cnp1-1, an S. pombe CENP-A mutant. While depletion of Ams2 results in the reduction of CENP-A binding to the centromere and chromosome missegregation, increasing its dosage restores association of a CENP-A mutant protein with centromeres. Conversely, overexpression of CENP-A or histone H4 suppresses an ams2 disruptant. The intracellular amount of Ams2 thus affects centromeric nucleosomal constituents. Ams2 is abundant in S phase and associates with chromatin, including the central centromeres through binding to GATA-core sequences. Ams2 is thus a cell cycle-regulated GATA factor that is required for centromere function.
authors.affiliation: Department of Biophysics, Graduate School of Science, Kyoto 606-8502, Japan.
authors.forename: Ee Sin
authors.initials: ES
authors.lastname: Chen
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Saitoh
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yanagida
authors.forename: Kohta
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D002503:Centromere / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D002875:Chromosomes / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D050980:GATA Transcription Factors; D006657:Histones / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D010641:Phenotype; D011485:Protein Binding; D012568:Schizosaccharomyces / Q000166:cytology / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: C472167:Ams2 protein, S pombe; D002868:Chromosomal Proteins, Non-Histone; C410349:Cnp1 protein, S pombe; D004268:DNA-Binding Proteins; D050980:GATA Transcription Factors; D006657:Histones; D029702:Schizosaccharomyces pombe Proteins