[Cytocompatibility of three substituted materials of human beings' hard tissue].
OBJECTIVE
To evaluate the osteocompatibility of three substituted materials of human beings' hard tissue (hydroxyapatite, bioglass ceramic and Ti-6Al-4V) and a new experimental method for evaluating the osteocompatibility of replaced materials of hard tissues in vitro.


METHODS
The isolated osteoblasts from parietal and frontal bones of Sparague Dawley rats from 1 day to 3 days were used to estimate osteocompatibility of three substituted materials in vitro. The cells were inoculated onto specimens of HA, BGC and Ti-6Al-4V for 1 day, 3, 5 and 7 days, then the morphology and proliferating rates of cells on different materials were tested.


RESULTS
Rat osteoblasts could attach, spread and proliferate on three materials as normal cells, and proliferating rates of cells on HA and BGC surface were as the same as normal, but that on Ti-6Al-4V was lower than the normal cells.


CONCLUSIONS
The osteocompatibility of HA and BGC are better than that of Ti-6Al-4V and rat osteoblast culture system can be used to evaluate the osteocompatibility of substituted materials in vitro.
Tang
Z
Z
Department of Stomatology, Medical College of Jinan University.
Chen
Z
Z
D000497:Alloys; D000818:Animals; D001672:Biocompatible Materials; D002455:Cell Division; D003776:Dental Porcelain; D006882:Hydroxyapatites; D066298:In Vitro Techniques; D010006:Osteoblasts / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014025:Titanium
D000497:Alloys; D001672:Biocompatible Materials; D006882:Hydroxyapatites; D003776:Dental Porcelain; C031462:titanium alloy (TiAl6V4); D014025:Titanium