title: [Separation and determination of 1,5-benzothiazepines-alpha-chloro-beta-lactam with thin-layer chromatography].
abstract: A method for separation and determination of 1,5-benzothiazepines-alpha-chloro-beta-lactam in reaction mixture by thin-layer chromatography has been established. The operating conditions were: lambda 1 = 270 nm, lambda 2 = 310 nm, beam size: 1.2 mm x 1.2 mm, SX = 3. The calibration curve was linear in the range of 0.285-5.7 micrograms (r = 0.9952, n = 8) and the detectable limit was 0.114 microgram. The coefficient of variation of this method was less than 5% and the average recovery for beta-lactam was 96.75%. The method is simple and rapid.
authors.affiliation: Department of Chemistry, Hebei Normal University, Shijiazhuang 050016, China.
authors.forename: N
authors.initials: N
authors.lastname: Sun
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: J Y
authors.initials: JY
authors.lastname: Wang
authors.forename: C Y
authors.initials: CY
authors.lastname: Du