title: [Determination of angiotensin-converting enzyme activity by micellar electrokinetic capillary chromatography].
abstract: A sensitive and rapid method was developed for angiotensin-converting enzyme (ACE) activity determination by micellar electrokinetic capillary chromatography (MECC). MECC was carried out to separate and quantify the products of the enzymatic reacting using Hip-Leu-His as the substrate in 20 mmol/L boric acid-borate buffer (pH 9.0) including 50 mmol/L SDS as the run buffer at an applied voltage of 8.1 kV. The electrophoresis was monitored at 228 nm, and completed in 6 minutes. The detection limits of ACE activity was 5 pmol/min(signal to noise ratio was 2).
authors.affiliation: Institute of Biotechnology, Fuzhou University, Fuzhou 350002, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Xu
authors.forename: R Z
authors.initials: RZ
authors.lastname: Zhang
authors.forename: S M
authors.initials: SM
authors.lastname: Sheng
authors.forename: T B
authors.initials: TB
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: P F
authors.initials: PF
authors.lastname: Rao
mesh_terms: D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary; D007703:Peptidyl-Dipeptidase A / Q000032:analysis* / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: D002021:Buffers; D007703:Peptidyl-Dipeptidase A