Superbeads: immobilization in "sweet" chemistry.
Enzymatic oligosaccharide synthesis using recombinant glycosyltransferases is able to overcome the difficulties associated with chemical methods. Nonetheless, sugar nucleotide regeneration cycles are necessary for the glycosylation. The multistep enzyme reaction can be efficiently carried out on superbeads that are prepared by immobilizing multienzyme mixtures on bead support through fused binding domains.
Nahalka
Jozef
J
Institute of Chemistry, Slovak Academy of Sciences Dúbravská cesta 9 SK-832 32 Bratislava, Slovak Republic.
Liu
Ziye
Z
Chen
Xi
X
Wang
Peng George
PG
D001709:Biotechnology / Q000379:methods; D002236:Carbohydrate Conformation; D002384:Catalysis; D018914:Cells, Immobilized; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D016695:Glycosyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008863:Microspheres; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000378:metabolism
D004800:Enzymes, Immobilized; D009844:Oligosaccharides; D016695:Glycosyltransferases