[Screening and characteristics of mutants of E. coli resistant to acetate inhibition].
Acetate is accumulated in the aerobic high cell density culture of Escherichia coli, Which seriously inhibits cell growth and expression of recombinant proteins. To alleviate acetate inhibition, mutants of E. coli JM101 were generated by 60Co radiation and then enriched in continuous culture with acetate as the selective pressure. One of the mutants isolated, JL3, showed obvious increased tolerance toward acetate and maintained phenotypic stability on slant without acetate. In MA medium containing 10 g/L of sodium acetate, the specific growth rate and the glucose consumption rate of JL3 were higher than those of JM101.
Li
Z
Z
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Ye
Q
Q
D003037:Cobalt Radioisotopes; D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism / Q000528:radiation effects; D005947:Glucose / Q000378:metabolism; D009154:Mutation; D019346:Sodium Acetate / Q000378:metabolism*
D003037:Cobalt Radioisotopes; D003470:Culture Media; D019346:Sodium Acetate; D005947:Glucose