[Relationship between the expression of Bax and apoptosis induced by hyperthermia in BcaCD885 cells].
OBJECTIVE
This study aimed to investigate the role of Bax gene in apoptosis induced by hyperthermia in BcaCD885 cells.


METHODS
After inducing apoptosis through hyperthermia, the expression of Bax protein and mRNA were examined with FCM antibody analysis and RT-PCR technique respectively.


RESULTS
Up regulation of Bax protein was noted after hyperthermia. The correlation coefficient r between the apoptotic rate and the expression Bax protein was 0.9240. The expression of Bax mRNA was also raised after hyperthermia.


CONCLUSIONS
Hyperthermia acting as an external signal may up-regulates expression of Bax gene and then induces apoptosis in BcaCD885 cells.
He
Y
Y
First Affiliated Hospital of Kunming Medical College.
Mao
Z
Z
Bian
L
L
Liang
X
X
Gao
Z
Z
Zuo
H
H
Cao
X
X
D017209:Apoptosis / Q000502:physiology*; D006801:Humans; D006979:Hyperthermia, Induced; D009062:Mouth Neoplasms / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D019253:Proto-Oncogene Proteins c-bcl-2; D014407:Tumor Cells, Cultured; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein