[Effects of fluid shear stress on PGE2 synthesis of primary osteoblast-like cells in rats].
OBJECTIVE
The purpose of this study is to study the function of PGE2 during the signal transduction in which fluid shear stress regulates the proliferation and differentiation of osteoblast-like cells.


METHODS
The isolated rat primary osteoblast-like cells were exposed to fluid shear stress 0.12 mN/cm2 for 5, 10, 15, 30, 60 and 120 minutes respectively in a flow chamber. The release of PGE2 was examined.


RESULTS
After exposure to fluid shear stress, the PGE2 synthesis of rat primary osteoblast-like cells increased significantly (P < 0.01) when compared with the control. After 10 minutes, the release of PGE2 began to increase significantly (P < 0.01) and the effect was maximal after 60 minutes (P < 0.01).


CONCLUSIONS
PGE2 pathway may be one of the signal-transduction pathways which can transduce the fluid shear stress into osteoblast-like cells and then stimulate the bone remodeling.
Qiao
J
J
College of Stomatology, West China University of Medical Sciences.
Chen
W
W
Li
S
S
Luo
S
S
Tian
W
W
Zhang
M
M
Li
Q
Q
D000818:Animals; D016723:Bone Remodeling; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis*; D010006:Osteoblasts / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012212:Rheology; D033081:Shear Strength; D015398:Signal Transduction / Q000502:physiology
D015232:Dinoprostone