[Conjugational actinomycetes of plasmid RSF101 from Escherichia coli to the rare actinomyceces of Nocardia asteriodes and Streptoverticillum caespitosus].
RSF1010 is a naturally occurring Escherichia coli broad host-range plasmid about 8.7 kb in size. It can be mobilized at high frequency between different gram-negative bacterial species when transfer functions are available in trans. Following the pioneering work of conjugational transfer of RSF1010 from E. coli to Streptomyces lividans and Mycobacterium smegmatis, the transfer of this plasmid by conjugation from E. coli S17.1 tp two gram-positive rare actinomycetes, Nocardia asteroides 3927 and Streptoverticillum caespitosus ATCC27422 was first time reported in this study. Southern blot analysis of the total DNA extracted from the actinomycetes' exconjugants proved that RSF1010 had been transferred from E. coli into the two new hosts and maintained staby in the exconjugants. Meanwhile, partial deletions of RSF1010 replicon loosing its antibiotics resistance makers were readily detected in E. coli. The implenmentation of this observation was discussed.
Li
W
W
Laboratory of Molecular Regulation for Microbial Secondary Metabolism, Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai 200032.
Shen
M
M
Jiao
R
R
Zhao
G
G
D000190:Actinomyces / Q000235:genetics*; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics*; D009616:Nocardia asteroides / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D018741:Replication Origin / Q000235:genetics; D013302:Streptomyces / Q000235:genetics*; D014169:Transformation, Bacterial