title: Comparative genome- and proteome analysis of cerebral cortex from MK-801-treated rats.
abstract: cDNA microarrays and two-dimensional gel-electrophoresis in combination with mass spectrometry, were used to screen alterations in mRNA and protein levels, respectively, in cerebral cortex of MK-801-treated rats. The rats were divided in two groups; group 1 (short-term treated) and group 2 (long-term treated). In group 1, four genes were up-regulated and five down-regulated. In group 2, seven genes were up-regulated and six down-regulated. In group 1, the levels of one protein was increased and eight proteins reduced. In group 2, the levels of two proteins were increased and four proteins reduced. Several of the altered genes (casein kinase 2, glutamic acid decarboxylase, synaptotagmin, gamma aminobutyric acid [GABA] transporter, creatine kinase, and cytochrome c oxidase) and proteins (superoxide dismutase, hsp 60, hsp 72 and gamma-enolase) have previously been connected to schizophrenia. Alterations of the genes (microglobulin, c-jun proto-oncogene, 40S ribosomal protein S19, adenosine diphosphate (ADP)-ribosylation factors, platelet-derived growth factor, fructose-bisphophate aldolase A, and myelin proteolipid) and the proteins (stathmin, H+-transp. Adenosine triphosphate (ATP) synthase, pyruvate dehydrogenase, beta-actin and alpha-enolase), have not, to our knowledge, earlier been implicated in schizophrenia pathology. Overall, these results with a combined approach of genomics and proteomics add to the validity of subchronic N-methyl-D-aspartate (NMDA)-receptor antagonist treatment as an animal model of schizophrenia.
authors.affiliation: Department of Clinical Neuroscience, Göteborg University, Göteborg, Sweden. linda.paulson@neuro.gu.se
authors.forename: Linda
authors.initials: L
authors.lastname: Paulson
authors.forename: Peter
authors.initials: P
authors.lastname: Martin
authors.forename: Anders
authors.initials: A
authors.lastname: Persson
authors.forename: Carol L
authors.initials: CL
authors.lastname: Nilsson
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Ljung
authors.forename: Ann
authors.initials: A
authors.lastname: Westman-Brinkmalm
authors.forename: Peter S
authors.initials: PS
authors.lastname: Eriksson
authors.forename: Kaj
authors.initials: K
authors.lastname: Blennow
authors.forename: Pia
authors.initials: P
authors.lastname: Davidsson
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D016678:Genome; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D020543:Proteome / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012559:Schizophrenia / Q000235:genetics / Q000378:metabolism
chemical_list: D020543:Proteome; D012333:RNA, Messenger; D016291:Dizocilpine Maleate