title: Determination of riboflavin in urine by capillary electrophoresis-blue light emitting diode-induced fluorescence detection combined with a stacking technique.
abstract: A simple, inexpensive and reliable method for the simultaneous, routine analysis of riboflavin in urine by capillary electrophoresis-light emitting diode (LED)-induced fluorescence detection is described. Using a blue LED as the light source, the detection limit of riboflavin was determined to be 0.48 microg/ml and was improved to 20 ng/ml when a stacking technique was applied. In the analysis of an actual sample, various concentrations of riboflavin were distributed in the urine samples over a period of 9 h after the ingestion of a vitamin B(2) tablet.
authors.affiliation: Department of Chemistry, National Taiwan Normal University, 88 Sec. 4, Tingchow Road, Taipei, Taiwan.
authors.forename: An-Kai
authors.initials: AK
authors.lastname: Su
authors.forename: Cheng-Huang
authors.initials: CH
authors.lastname: Lin
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D012256:Riboflavin / Q000652:urine*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D012256:Riboflavin