[Determination of salicylic acid and its hydroxylated products using high performance liquid chromatography and fluorescence detection].
High performance liquid chromatography (HPLC) with fluorescence detector was used to determine salicylic acid (SA) and its main hydroxylated products, 2,3-dihydroxybenzoic acid (2,3-DHBA) and 2,5-dihydroxybenzoic acid (2,5-DHBA). Mobile phase was 30 mmol/L acetate(pH 4.9) and the flow rate was 1.0 mL/min. Low detection limits, 10 nmol/L for 2,3-DHBA, 0.6 nmol/L for 2,5-DHBA and 1.0 nmol/L for SA were obtained. The photolysis system of salicylic acid and H2O2 was investigated. The products in the system were measured and the yields were 45% and 51% for 2,3-DHBA and 2,5-DHBA, respectively.
Ren
X R
XR
State Key Laboratory of Environmental Simulation and Pollution Control, Center of Environmental Sciences, Peking University, Beijing 100871, China.
Shao
K S
KS
Tang
X Y
XY
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006900:Hydroxylation; D020156:Salicylic Acid / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D020156:Salicylic Acid