[Simultaneous determination of magnolol and honokiol in serum and urine by high performance liquid chromatography].
A reversed-phase high performance liquid chromatographic method for simultaneous determination of magnolol and honokiol in serum and urine of rat has been established. Two drugs were determined within 15 minutes by the method on the column with spherisorb C18, by using a mobile phase consisted of methanol-water-glacial acetic acid (70:30:1, V/V) at 1 mL/min, monitored at 294 nm and with a sensitivity of 0.005 AUFS. After 0.25, 1 and 8 hour of administration of the drugs, protein in serum and urine of Wistar rat was precipitated by methanol and magnolol and honokiol in acidified body fluid were determined after being extracted by a mixture of ethyl acetate and ether. Good linear relationship between concentration in serum and urine and peak area in the ranges of 0.05-2 mg/L for magnolol and 0.025-1 mg/L for honokiol was obtained. Good precision and reproducibility were found too. The average recoveries of the two drugs were 95.6% (RSD = 3.85%), 93.8% (RSD = 3.95%) in serum and 96.0% (RSD = 3.83%), 94.9% (RSD = 3.54%) for urine respectively. The lower limit of the method was 0.02 mg/L of magnolol and 0.04 mg/L of honokiol respectively. The results showed that this method is suitable for the determination of magnolol and honokiol in body fluids.
Yuan
C
C
Department of Clinical Pharmacology, General Hospital of Jinan Military Region, Jinan 250031, China. yuanchen@jn-public.sd.cninfo.net
Du
K
K
Zhu
L Q
LQ
Wang
J X
JX
D000818:Animals; D001713:Biphenyl Compounds / Q000097:blood* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D017705:Lignans; D031566:Magnolia / Q000737:chemistry*; D008297:Male; D010975:Platelet Aggregation Inhibitors / Q000097:blood / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D000498:Allyl Compounds; D010636:Phenols
D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D017705:Lignans; D010975:Platelet Aggregation Inhibitors; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols