Opsin cDNA sequences of a UV and green rhodopsin of the satyrine butterfly Bicyclus anynana.
The cDNAs of an ultraviolet (UV) and long-wavelength (LW) (green) absorbing rhodopsin of the bush brown Bicyclus anynana were partially identified. The UV sequence, encoding 377 amino acids, is 76-79% identical to the UV sequences of the papilionids Papilio glaucus and Papilio xuthus and the moth Manduca sexta. A dendrogram derived from aligning the amino acid sequences reveals an equidistant position of Bicyclus between Papilio and Manduca. The sequence of the green opsin cDNA fragment, which encodes 242 amino acids, represents six of the seven transmembrane regions. At the amino acid level, this fragment is more than 80% identical to the corresponding LW opsin sequences of Dryas, Heliconius, Papilio (rhodopsin 2) and Manduca. Whereas three LW absorbing rhodopsins were identified in the papilionid butterflies, only one green opsin was found in B. anynana.
Vanhoutte
K J A
KJ
Department of Neurobiophysics, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. vanhoutte@phys.rug.nl
Eggen
B J L
BJ
Janssen
J J M
JJ
Stavenga
D G
DG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002080:Butterflies / Q000145:classification / Q000235:genetics*; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D007313:Insecta; D008027:Light; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012243:Rhodopsin / Q000032:analysis*; D017299:Rod Opsins / Q000235:genetics* / Q000528:radiation effects; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014466:Ultraviolet Rays
D017931:DNA Primers; D018076:DNA, Complementary; D017299:Rod Opsins; D012243:Rhodopsin