title: Self-assembled DNA-conjugated polymer for novel DNA chip.
abstract: We developed DNA-conjugated polymer for DNA chip fabrication. A 30 mer probe DNA and disulfide bridges were covalently attached to the polymer side chain. The DNA-conjugated polymer can be specifically adsorbed on a gold substrate surface by a self-assembly technique. The interaction between fully matched DNA and DNA-conjugated polymer was investigated by surface plasmon resonance (SPR) technique. The DNA-conjugated polymer-modified gold surface highly recognized fully matched DNA, rather than unmatched DNA. Therefore, DNA-conjugated polymer can be used for novel DNA chip fabrication.
authors.affiliation: Laboratory of Advanced Bioelectronics, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 4, 1-1-1 Higashi, Tsukuba 305-8562, Japan.
authors.forename: Shu
authors.initials: S
authors.lastname: Taira
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Morita
authors.forename: Eiichi
authors.initials: E
authors.lastname: Tamiya
authors.forename: Kenji
authors.initials: K
authors.lastname: Yokoyama
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D006863:Hydrogen-Ion Concentration; D008904:Miniaturization; D009693:Nucleic Acid Hybridization; D011108:Polymers / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D015342:DNA Probes; D011108:Polymers; D004247:DNA