Rat erythrocytes express the anionic amino acid transport protein EAAC1.
EAAC1 is a widely expressed protein which transports anionic amino acids in a Na(+)-dependent fashion. Rat erythrocytes have generally been thought to be impermeant to anionic amino acids. Utilizing immunoelectron microscopy, we have demonstrated the presence of EAAC1-immunoreactive protein within rat erythrocytes. Immunoblotting revealed the presence of an approximately 60-kDa protein, consistent with EAAC1, in erythrocyte membranes. Specificity was confirmed by peptide competition. In conclusion, EAAC1 is expressed in rat erythrocytes.
Novak
Donald
D
Division of Pediatric Gastroenterology and Hepatology, University of Florida College of Medicine, Gainesville, FL 32610, USA. novakda@peds.ufl.edu
Beveridge
Mark
M
Verlander-Reed
Jill
J
D027322:Amino Acid Transport System X-AG / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D004912:Erythrocytes / Q000378:metabolism*; D050580:Excitatory Amino Acid Transporter 3; D050491:Glutamate Plasma Membrane Transport Proteins; D007150:Immunohistochemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D027981:Symporters / Q000096:biosynthesis / Q000235:genetics*
D027322:Amino Acid Transport System X-AG; D050580:Excitatory Amino Acid Transporter 3; D050491:Glutamate Plasma Membrane Transport Proteins; C493398:Slc1a1 protein, rat; D027981:Symporters