title: Kinetics of CH4 oxidation in mixed culture.
abstract: Microbial cultures used for methane oxidation experiments were prepared by enriching under a 20 % CH4 atmosphere either leachate extracts from two different landfill sites or a digested sewage sludge. No microbes were axenically isolated, therefore the cultures were mixtures of CH4 oxidising and other aerobic microorganisms. CH4 oxidation experiments were carried out with a batch reactor, whose change in gas volume could be measured. A mixture of air and methane with CH4 concentrations ranging from 5 to 20 % was introduced into the reactor. Methane oxidation rate was expressed as a Monod equation for CH4 and a first order reaction for O2. Maximum oxidation rate (Vmax) resulted 2.1 x 10(-13) to 3.4 x 10(-11) hr(-1) cell(-1), and half saturation constant (KM) was 5.1 x 10(-5) to 6.5 x 10(-4) mol L(-1). The molar ratio of consumed O2 to oxidised CH4 was 1.5-1.8
authors.affiliation: Department of Civil and Environmental Engineering, Miyazaki University, 1-1 Gakuenn Kibanadai Nishi, Miyazaki 889-2192, Japan. dote@civil.miyazaki-u.ac.jp
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Dote
mesh_terms: D001419:Bacteria; D017752:Greenhouse Effect; D007700:Kinetics; D008697:Methane / Q000737:chemistry*; D008962:Models, Theoretical; D010084:Oxidation-Reduction; D012722:Sewage / Q000737:chemistry / Q000382:microbiology
chemical_list: D012722:Sewage; D008697:Methane