[Cloning, sequencing of beagle E-selectin genomic DNA and comparison of it with other species].
OBJECTIVE
Selectins are a group of members of Cell Adhesion Molecular family; they play very important roles in inflammation and neovascularization. The aim of this study is to clone, sequence Beagle E-selectin genomic DNA and make homogeneous analyses to compare it with those of other animals.


METHODS
Twelve fragments of genomic DNA of Beagle E-selectin were obtained by PCR, and the 12 fragments were cloned in T-vector. At last, the genomic DNA sequences of Beagle E-selectin were obtained through gene sequencing, assembling and base calling, and the gene structure was analyzed.


RESULTS
The sequence of Beagle E-selectin genomic DNA that contained the whole open reading box was acgured. The results of homogeneous analyses showed a great extent of E-selectin gene homology among human, porcine and dog.


CONCLUSIONS
The demonstration of a large extent of the gene homology has substantiated in this connection the important roles of E-selectin in transplantation immunity and neoplasm development.
Zheng
L
L
Department of Cardiovascular & Thoracic Surgery, First Affiliated Hospital, WCUMS, Chengdu 610041, China.
Shi
Y
Y
Dong
L
L
Zhang
G
G
Wu
H
H
Li
Y
Y
Cheng
S
S
D000818:Animals; D003001:Cloning, Molecular; D004285:Dogs; D019040:E-Selectin / Q000235:genetics* / Q000502:physiology; D016678:Genome; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine
D019040:E-Selectin