[Introduction of the chromogenic gene to the plant growth-promoting rhizobacteria of cucumber].
Using a bicomponent transposition system with the E. coli lacZY gene cloned between Tn7 termini, a sensitive, selectable marker based on expression of the E. coli lac operon genes encoding beta-galactosidase and lactose permease was transformed into the rifampicin resistant mutant of plant growth-promoting rhizobacteria of cucumber, Pseudomonas aeruginosa CN116 and Pseudomonas corrugata CN31, respectively. Transformants were conferred the ability to utilize lactose as a sole carbon source and the ability to cleave the chromogenis substrate X-Gal to show a specific blue color. Southern blotting analysis showed that lacZY gene was inserted into the genome DNA of target strains. Compared with the wild type strains, the cultural characters, morphological features, growth promoting and disease control effects of transformants were almost unchanged, except the new marked phenotype. This marker system enabled the detection of lac+ transformants at sensitivity of 10 CFU/g soil, which makes the further studies on PGPR more easily.
Chen
X
X
Department of Plant Protection, Zhejiang University, Hangzhou, 310029, China.
Zhang
B
B
Lou
B
B
Ryder
M H
MH
D018553:Cucumis sativus / Q000382:microbiology*; D004251:DNA Transposable Elements / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D007763:Lac Operon; D026901:Membrane Transport Proteins / Q000235:genetics; D011549:Pseudomonas / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000235:genetics; D012231:Rhizobium / Q000235:genetics*; D014170:Transformation, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D004251:DNA Transposable Elements; D026901:Membrane Transport Proteins; C021499:lactose permease; D001616:beta-Galactosidase