Reference isolates for the clonal complexes of Campylobacter jejuni.
OBJECTIVE
To identify and make available through the National Collection of Type Cultures (NCTC) a set of reference isolates for the clonal complexes of Campylobacter jejuni.


RESULTS
The development of a multilocus sequence typing scheme for C. jejuni enabled the genetic characterization of a large number of isolates (n = 814) from cases of human disease, animals, birds and their food products. The nucleotide sequence data were used to assign each isolate an allelic profile or sequence type (ST) and examine the C. jejuni population structure in terms of clonal complexes. The clonal complexes consisted of an abundant central or founder genotype (ST), after which the complex was named, together with very closely related, generally less abundant genotypes differing from the founder at one, two or three loci. The clonal complex is an informative unit for the study C. jejuni epidemiology. It provides data which enabled the choice of 13 C. jejuni founder isolates for submission to the NCTC as a representative cross-section of the C. jejuni population.


CONCLUSIONS
These 13 isolates provide a defined resource for further research into aspects of C. jejuni biology such as genomic diversity, virulence and adaptation to particular hosts or environmental survival.


CONCLUSIONS
This isolate collection is available through the NCTC and provides a resource for further research.
Wareing
D R A
DR
The Public Health Laboratory, Royal Preston Hospital, P.O. Box 202, Preston PR2 9HG, UK. dwareing@nw.phls.nhs.uk
Ure
R
R
Colles
F M
FM
Bolton
F J
FJ
Fox
A J
AJ
Maiden
M C J
MC
Dingle
K E
KE
D000483:Alleles; D000818:Animals; D015373:Bacterial Typing Techniques; D002169:Campylobacter Infections / Q000382:microbiology; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle; D002645:Chickens; D002999:Clone Cells; D004269:DNA, Bacterial / Q000032:analysis; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D012015:Reference Standards; D017422:Sequence Analysis, DNA; D012756:Sheep
D004269:DNA, Bacterial