The human MC2-R gene expression: different aspects of its control.
Expression of the adrenocorticotropin receptor (MC2-R) is restricted to adrenocortical cells and is up-regulated by both adrenocorticotropin and angiotensin II through the activation of protein kinase A and protein kinase C pathways, respectively. After cloning of the promoter region of the human MC2-R gene (hMC2-R), we have shown that cyclic AMP-induced regulation of transcriptional activity of the gene is achieved through two SF1 binding elements located in the proximal promoter. On the other hand, regulation by angiotensin II partly involved two AP1 binding sites. Using different primary cell cultures, we have also been able to delineate a region inside the promoter which is responsible for part of the tissue-specific expression of the gene.
Blondet
A
A
INSERM U418/INRA UA953, Hôpital Debrousse, 29 Rue Soeur Bouvier, 69322 Lyon Cedex 05, France.
Doghman
M
M
Penhoat
A
A
Durand
P
P
Bégeot
M
M
Naville
D
D
D000804:Angiotensin II / Q000494:pharmacology; D001665:Binding Sites / Q000235:genetics / Q000502:physiology; D000242:Cyclic AMP / Q000502:physiology; D004268:DNA-Binding Proteins / Q000378:metabolism*; D051717:Fushi Tarazu Transcription Factors; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins; D006801:Humans; D009154:Mutation / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000502:physiology; D044103:Receptor, Melanocortin, Type 2; D018043:Receptors, Corticotropin / Q000235:genetics*; D018160:Receptors, Cytoplasmic and Nuclear; D054339:Steroidogenic Factor 1; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D051717:Fushi Tarazu Transcription Factors; D018398:Homeodomain Proteins; D044103:Receptor, Melanocortin, Type 2; D018043:Receptors, Corticotropin; D018160:Receptors, Cytoplasmic and Nuclear; D054339:Steroidogenic Factor 1; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D000804:Angiotensin II; D000242:Cyclic AMP