Intraepithelial infiltration of eosinophils and their contribution to the elimination of adult intestinal nematode, Strongyloides venezuelensis in mice.
Eosinophils were examined for the capacity of attacking Strongyloides venezuelensis adult worms in the intestinal mucosa by using interleukin (IL)-5 transgenic mice. In IL-5 transgenic mice, most of the subcutaneously inoculated infective larvae were killed during migration, and only a few worms could reach the small intestine. When the same number of adult worms were surgically implanted in the small intestine of IL-5 transgenic and control mice, fecal egg output as well as the number of adult worms recovered from the intestine was significantly lower in IL-5 transgenic mice. In the intestinal mucosa of IL-5 transgenic mice, large number of eosinophils was present in the lamina propria even before adult worm implantation. The number of eosinophils increased significantly as early as 24 h after implantation and tripled by day 3, whereas mucosal eosinophilia remained low in wild-type mice. Most notably, eosinophils infiltrated into the intestinal epithelium and surrounded adult worms in IL-5 transgenic mice, which was never seen in wild-type control mice. However, IL-5 transgenic mice required the same period as normal mice to completely expel implanted adult worms. The amount of specific IgA as well as total IgA in the stool was high in IL-5 transgenic mice before adult worm implantation, and dropped rapidly after adult worm implantation. The present study suggests that eosinophils are capable of attacking adult nematodes in the intestinal epithelia, probably in conjunction with secretory IgA, although they are not enough for the complete worm expulsion.
El-Malky
Mohamed
M
Department of Molecular Parasitology, Nagoya City University Graduate School of Medical Sciences, Kawasumi-1, Mizuho, Nagoya 467-8601, Japan.
Maruyama
Haruhiko
H
Hirabayashi
Yoshiaki
Y
Shimada
Shoichi
S
Yoshida
Ayako
A
Amano
Teruaki
T
Tominaga
Akira
A
Takatsu
Kiyoshi
K
Ohta
Nobuo
N
D000818:Animals; D002465:Cell Movement; D004804:Eosinophils / Q000276:immunology*; D004847:Epithelial Cells / Q000276:immunology; D005243:Feces / Q000469:parasitology; D006790:Host-Parasite Interactions; D007070:Immunoglobulin A / Q000032:analysis; D015848:Interleukin-5 / Q000235:genetics; D007413:Intestinal Mucosa / Q000276:immunology / Q000469:parasitology* / Q000473:pathology; D007422:Intestines / Q000469:parasitology / Q000473:pathology; D008018:Life Cycle Stages; D051379:Mice; D008822:Mice, Transgenic; D009348:Nematoda / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification; D010270:Parasite Egg Count; D013321:Strongyloides / Q000145:classification / Q000254:growth & development / Q000276:immunology; D013322:Strongyloidiasis / Q000276:immunology* / Q000469:parasitology; D013997:Time Factors
D007070:Immunoglobulin A; D015848:Interleukin-5