[Influence of human cytomegalovirus infection on the expressions of HOXB1, HOXB5, HOXB6, and HOXB9 genes in human embryo lung cells].
OBJECTIVE
To study the expressions of HOXB1, HOXB5, HOXB6, and HOXB9 genes in human embryo lung (HEL) cells and influence of human cytomegalovirus (HCMV) infection on the expressions of these genes.


METHODS
The expressions of HOXB1, HOXB5, HOXB6, and HOXB9 genes were detected with semi-quantitative RT-PCR method.


RESULTS
1. HEL cells expressed HOXB5 and HOXB6 genes, but they did not express HOXB1 and HOXB9 genes. 2. After HCMV infection, HEL cell was induced to express HOXB9 gene, as the expression of HOXB6 gene was elevated. The expression of HOXB5 gene had no significant change. HOXB1 was still not expressed. 3. Treated with all-trans retinoic acid (ATRA), the expression of HOXB9 gene in HEL cells infected by HCMV was significantly increased. But during the advanced infect period, the expression of HOXB6 was significantly decreased.


CONCLUSIONS
HCMV can induce abnormal expressions of HOXB6 and HOXB9 genes, which may play an important role in HCMV-induced abnormal embryogenesis.
Chen
L Y
LY
Department of Microbiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Wu
G J
GJ
Dai
G
G
D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000472:pathogenicity; D003586:Cytomegalovirus Infections / Q000235:genetics*; D004622:Embryo, Mammalian; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008168:Lung / Q000166:cytology / Q000378:metabolism*
C101121:HOXB1 homeodomain protein; C489126:HOXB5 protein, human; C093252:HOXB6 protein, human; C491257:HOXB9 protein, human; D018398:Homeodomain Proteins; C490537:Hoxb6 protein, mouse