[Mitochondrial mechanisms of CPT-induced apoptosis in nasopharyngeal carcinoma cells].
OBJECTIVE
To understand whether CPT-induced apoptosis is related to the alteration of mitochondrial transmembrane potentials (delta psi m).


METHODS
A low differentiation human nasopharyngeal carcinoma (NPC) cell line of CNE-2Z cells were used as in vitro model. After treatment with 2 mumol/L CPT for 24 h, the delta psi m was detected by the staining of rhodamine 123 (Rh123), while apoptosis was confirmed by sub-G1 cell content and the double staining of hoechst33258 and propidium iodide (PI).


RESULTS
CPT induced delta psi m collapse and apoptosis. With 2 mumol/L CPT treatment for 24 h, delta psi mlow cell was increased to (19.0 +/- 3.0)%, while in the DMSO control, delta psi mlow was (7.0 +/- 1.4)% P < 0.05.


CONCLUSIONS
The disruption of the delta psi m may be one of the mechanisms of CPT-induced apoptosis in CNE-2Z cells.
Zhang
Y
Y
Department of Otorhinolaryngology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001.
Chen
Y
Y
Zhou
K
K
Cai
K
K
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002166:Camptothecin / Q000494:pharmacology*; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D002166:Camptothecin