Assessment of training effects on activity levels of alveolar macrophage in matured rats using chemiluminescent technique.
Chemiluminescence responses have been used for the evaluation of phagocyte function. In this study, to evaluate effects of training started after maturation on pulmonary immunity, the activity levels of rat alveolar macrophages (AMs) were assessed as reactive oxygen species (ROS) generating capacity, measured by lucigenin- and luminol-dependent chemiluminescence, using a parallel luminometer. One group of male Wistar rats started training at 11 weeks old and another group at 17 weeks old. The experimental period was 12 weeks, and about half of the rats were sacrificed after 6 weeks. The forced and voluntary exercises affect the mean levels of body weights and cell populations in the bronchoalveolar lavage fluid in younger animals; however, the voluntary exercise group in younger animals seemed to adapt after 12 weeks. By contrast, chemiluminescence responses in older rats observed after 6 weeks suggest that AMs are primed, and the maximum releasing activities of ROS are reduced. These changes in AM activity may be caused by the exercise and separation stresses and the rats may adapt to those stressors after 12 weeks. The chemiluminescent technique is thought to be useful to evaluate the changes of AM activity.
Kumae
Takashi
T
Division of Health Promotion and Exercise, National Institute of Health and Nutrition, Tokyo, Japan. kumae@nih.go.jp
Arakawa
Hatsuko
H
D000166:Acridines / Q000737:chemistry; D000367:Age Factors; D000818:Animals; D001835:Body Weight; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D007958:Leukocyte Count; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D016676:Macrophages, Alveolar / Q000737:chemistry / Q000502:physiology*; D008297:Male; D010805:Physical Conditioning, Animal / Q000502:physiology*; D005082:Physical Exertion / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000032:analysis*; D013312:Stress, Physiological / Q000276:immunology
D000166:Acridines; D017382:Reactive Oxygen Species; C033472:10,10'-dimethyl-9,9'-biacridinium; D008165:Luminol