title: Characterization of class I integrons in clinical strains of Salmonella enterica subsp. enterica serovars Typhimurium and Enteritidis from Norwegian hospitals.
abstract: The characterization of integrons and their promoters in 156 antibiotic-resistant clinical isolates of the important zoonotic pathogens Salmonella enterica subsp. enterica serovars Typhimurium (S. Typhimurium) and Enteritidis (S. Enteritidis) from Norwegian hospitals were performed. Integrons were found in 64 of 66 S. Typhimurium isolates (97 %) and in 20 of 90 S. Enteritidis isolates (22.2 %) with the following sizes; 650, 1000, 1200, 1500, 1600, 1700, 2000 and 2100 bp. The integrons were further sequenced and the aadA1, aadA2, aadA5, aadB, pse-1, catB3, oxa1, dfrA1, dfrA12 and dfrA17 genes, as well as a fragment of the sat1 gene, were found embedded in cassettes. An internal fragment of the purG gene was additionally found as an artefact PCR amplicon.
authors.affiliation: Norwegian Institute of Public Health, Division of Infectious Diseases Control, N-0403 Oslo, Norway. bjorn-arne.lindstedt@folkehelsa.no
authors.forename: Bjørn-Arne
authors.initials: BA
authors.lastname: Lindstedt
authors.forename: Even
authors.initials: E
authors.lastname: Heir
authors.forename: Irene
authors.initials: I
authors.lastname: Nygård
authors.forename: Georg
authors.initials: G
authors.lastname: Kapperud
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001434:Bacteriophage Typing; D001483:Base Sequence; D004269:DNA, Bacterial; D024881:Drug Resistance, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D006801:Humans; D032023:Integrons; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009664:Norway; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic / Q000235:genetics; D012480:Salmonella Infections / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000187:drug effects / Q000235:genetics*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial