[Cloning and identification of a new telomeric-associated zinc finger protein cDNA].
In order to isolate novel genes related to early human embryo development and differentiation, a directional cDNA library was constructed from 3-week-old human embryo. Single-pass DNA sequence analysis was used to sequence 47 randomly picked low-abundance cDNA clones. This approach led us to select a clone, L30, showing significant homology with the telomeric-associated DNA and zinc finger protein genes. It is about 3.8 kb in length and contains an open reading frame of notable length within 5'-region, and a tailing signal of AAUAAA and poly (A+) with 39 A in 3'-region. The gene was transcribed in human embryo by Northern blot hybridization and assigned to human chromosome 12 by in situ hybridization.
Gou
D M
DM
College of Life Sciences, Wuhan University, Wuhan 430072, China.
Li
W X
WX
Feng
Y D
YD
Xia
Z P
ZP
Gao
L
L
Jiang
D H
DH
D003001:Cloning, Molecular; D015342:DNA Probes; D018076:DNA, Complementary / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D020224:Expressed Sequence Tags; D005260:Female; D015723:Gene Library; D006801:Humans; D017403:In Situ Hybridization; D017422:Sequence Analysis, DNA; D016615:Telomere / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
D015342:DNA Probes; D018076:DNA, Complementary; D004268:DNA-Binding Proteins