title: Action potentials from human neuroblastoma cells in magnetic fields.
abstract: The patch-clamp method was used to measure transmembrane Na(+) and K(+) currents of the action potential in SH-SY5Y neuroblastoma cells exposed to static magnetic fields of 1, 5, and 75 G, 60 Hz fields of 1 and 5 G, and to combined static and low-frequency fields tuned for resonance of Na(+) and K(+). The maximum currents and their inactivation rates, and the activation rate of the Na(+) current were measured. Application of the magnetic fields did not result in detectable changes in any of the parameters of the action potential chosen for study. The occurrence of effects due to the fields could be excluded down to at least one part in 1000. The results suggest that magnetic fields of the type studied do not affect the cellular mechanisms responsible for generating the action potential.
authors.affiliation: Department of Orthopaedic Surgery, Louisiana State University Health Sciences Center, P.O. Box 33932, Shreveport, LA 71130-3932, USA.
authors.forename: Harold
authors.initials: H
authors.lastname: Sonnier
authors.forename: Oleg
authors.initials: O
authors.lastname: Kolomytkin
authors.forename: Andrew
authors.initials: A
authors.lastname: Marino
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D004574:Electromagnetic Fields; D006801:Humans; D017136:Ion Transport / Q000187:drug effects; D009447:Neuroblastoma / Q000378:metabolism / Q000503:physiopathology*; D018408:Patch-Clamp Techniques / Q000379:methods; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D019789:Tetraethylammonium / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D013779:Tetrodotoxin; D019789:Tetraethylammonium; D012964:Sodium; D011188:Potassium