title: [Immunohistochemical study on myocardial acute ischemia reperfusion injury in rats with anti-FOS protein antibody].
abstract: To investigate law of FOS protein induced by myocardial ischemia reperfusion (MI/R) in acute period, a model in 20 anaethetized SD rats was established. Rats with normal and ischemia were used as control groups. Specimens were studied immunohistochemically with c-fos antibody. After ischemia 20 minutes, followed by 30 minutes reperfusion, the area of MI/R showed nuclei of myocytes positive staining in cryosection slides. In C2 group, the area showed nuclei of myocytes (37.76% +/- 9.66%) positive staining weakly. In C3 group, nuclei of cardiac myocytes(40.34% +/- 3.32%) was significantly positive. In C4 group it began to attenuate(35.36% +/- 4.81%). The myocardium in normal and ischemic control groups showed negative staining. No changes were seen with HE staining. Our data indicated that immunohistochemical method may reveal acute MI/R injury of ischemia 20 min and reperfusion 30 min with anti-FOS protein staining and there is problely a peak between 60-120 min after reperfusion. It is possible that the method be used to diagnose sudden cardiac death in forensic medicine.
authors.affiliation: Department of Forensic Pathology, Sun Yat-Sen University of Medical Sciences, GuangZhou 510089, P. R. China.
authors.forename: M Y
authors.initials: MY
authors.lastname: Ma
authors.forename: X H
authors.initials: XH
authors.lastname: Xu
authors.forename: B
authors.initials: B
authors.lastname: Luo
authors.forename: J Z
authors.initials: JZ
authors.lastname: Zhu
authors.forename: J G
authors.initials: JG
authors.lastname: Chen
authors.forename: L X
authors.initials: LX
authors.lastname: Zhao
authors.forename: H Y
authors.initials: HY
authors.lastname: Wang
mesh_terms: D000818:Animals; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016760:Proto-Oncogene Proteins c-fos