title: Isolation and structure of the cytotoxic cycloheptapeptide phakellistatin 13.
abstract: A new cyclic heptapeptide phakellistatin 13 (1) had been isolated from the sponge Phakellia fusca Thiele, collected at Yongxing Island of China. Its structure was elucidated as cyclo-(Pro1-Trp-Leu-Thr-Pro2-Gly-Phe) on the basis of MS, UV, IR, and high-field NMR (600 MHz) analysis. The compound was significantly cytotoxic against the human hepatoma BEL-7404 cell line with an ED(50) < 10(-2) microg/mL.
authors.affiliation: Research Center for Marine Drugs, School of Pharmacy, Second Military Medical University, 325 Gou-He Road, Shanghai 200433, People's Republic of China.
authors.forename: Wen-Lin
authors.initials: WL
authors.lastname: Li
authors.forename: Yang-Hua
authors.initials: YH
authors.lastname: Yi
authors.forename: Hou-Ming
authors.initials: HM
authors.lastname: Wu
authors.forename: Qiang-Zhi
authors.initials: QZ
authors.lastname: Xu
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Tang
authors.forename: Da-Zheng
authors.initials: DZ
authors.lastname: Zhou
authors.forename: Hou-Wen
authors.initials: HW
authors.lastname: Lin
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Wang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular; D002681:China; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011161:Porifera / Q000737:chemistry*; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D010456:Peptides, Cyclic; C474532:phakellistatin 13