title: [Construction of bi-cistronic co-expression plasmid of mIL-12 and the expression in vitro or in vivo]
abstract: OBJECTIVE: To construct a bi-cistronic co-expression plasmid for mouse interleukin-12 and to observe its expression in vitro or in vivo.METHODS: The full-length cDNA encoding p35 and p40 was cloned into eukaryotic cells expression vector pcDNA 3.1 respectively. Subsequently,the p35 expression unit was inserted into pcDNA 3.1/p40 to produce the bi-cistronic co-expression plasmid in which the p35 and p40 genes were controlled by their own CMV.The plasmid was expressed in vitro and in vivo. RESULTS: The mIL-12 in the supernatant was detected by ELISA after the pCmIL-12 was transfected into COS-7 cells. The activity of NK cells could be augmented by the supernatant in vitro and also by by intradermal delivery of pCmIL-12 in vivo. CONCLUSION: The plasmid constructed by us can express biologically active mIL-12 in vitro and in vivo.
authors.affiliation: College of Medical Sciences, Zhejiang University, Hangzhou 310031, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Chuan-Sen
authors.initials: CS
authors.lastname: Shao
authors.forename: Jian-Gen
authors.initials: JG
authors.lastname: Shen
authors.forename: Jian-Fang
authors.initials: JF
authors.lastname: Bao
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Pan
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.forename: Lin
authors.initials: L
authors.lastname: Shou
authors.forename: Shao
authors.initials: S
authors.lastname: Xiang
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng