Identification of two nucleotide sequence sub-groups within Potato mop-top virus.
To evaluate the variation of Potato mop-top pomovirus from potato fields, 21 isolates were collected from different Danish locations. Reverse transcription-polymerase chain reaction-restriction fragment length polymorphism (RT-PCR-RFLP) of regions of RNA2 was performed for all 21 isolates resulting in the establishment of two sub-groups of isolates. The nucleotide sequence of a region encoding part of the 'readthrough protein' of RNA2 was compared for 9 of these isolates. This sequence analysis confirmed the RT-PCR-RFLP grouping. The isolates were tested for symptom expression in indicator plants and grouped according to symptom development. No correlation between grouping based on symptom development and genotype was observed.
Nielsen
S L
SL
Department of Plant Protection, Danish Institute of Agricultural Sciences, Research Centre Flakkebjerg, Slagelse, Denmark. SteenL.Nielsen@agrsci.dk
Nicolaisen
M
M
D001483:Base Sequence; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000145:classification / Q000235:genetics* / Q000502:physiology; D012150:Polymorphism, Restriction Fragment Length; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D011198:Solanum tuberosum / Q000821:virology*