No effects of estrogen receptor overexpression on gonadal sex differentiation and reversal in medaka fish.
In order to elucidate a possible role of estrogen receptor in the gonadal sex differentiation and the sex reversal with sex steroids, we examined for the formation of testis or ovary in transgenic medaka fish overexpressing the medaka estrogen receptor under the constitutive medaka beta-actin promoter. The transgenic fish underwent the genetically determined gonadal differentiation and showed the same sex-reversal rates as those of wild-type non-transgenic fish after treatments with estrogen and androgen. These results present invaluable data to reconsider the role of estrogen receptor in the gonadal sex determination.
Kawamura
Toshiyuki
T
Center for Gene Science, Hiroshima University, Kagamiyama 1-4-2, Higashi-hiroshima 739-8527, Japan.
Omura
Seiichi
S
Sakai
Shuichi
S
Yamashita
Ichiro
I
D000728:Androgens / Q000494:pharmacology / Q000502:physiology; D000818:Animals; D030801:Animals, Genetically Modified; D004967:Estrogens / Q000494:pharmacology / Q000502:physiology; D005260:Female; D015870:Gene Expression; D057850:Hermaphroditic Organisms; D008297:Male; D009990:Oryzias / Q000196:embryology* / Q000235:genetics*; D010053:Ovary / Q000196:embryology; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D019849:Sex Determination Processes; D013737:Testis / Q000196:embryology
D000728:Androgens; D004967:Estrogens; D011960:Receptors, Estrogen