Transgenic mice with hematopoietic and lymphoid specific expression of Cre.
Bacteriophage P1 Cre/loxP based systems can be used to manipulate the genomes ofmice in vivo and in vitro, allowing the generation of tissue-specific conditional mutants. We have generated mouse lines expressing Cre recombinase in hematopoietic tissues using the vav regulatory elements, or in lymphoid cells using the hCD2 promoter and locus control region (LCR). The R26R-EYFP Cre reporter mouse line was used to determine the pattern of Cre expression in each line and enabled the assessment of Cre activity at a single-cell level. Analysis showed that the vav promoter elements were able to direct Cre-mediated recombination in all cells of the hematopoietic system. The hCD2 promoter and LCR on the other hand were able to drive Cre-mediated recombination only in T cells and B cells, but not in other hematopoietic cell types. Furthermore, in the appropriate tissues, deletion of the floxed target was complete in all cells, thereby excluding the possibility of variegated expression of the Cre transgene. Both of these Cre-transgenic lines will be useful in generating tissue-specific gene deletions within all the cells of hematopoietic or lymphoid tissues.
de Boer
Jasper
J
Department of Molecular Immunology, National Institute for Medical Research, The Ridgeway, London, GB.
Williams
Adam
A
Skavdis
George
G
Harker
Nicola
N
Coles
Mark
M
Tolaini
Mauro
M
Norton
Trisha
T
Williams
Keith
K
Roderick
Kathleen
K
Potocnik
Alexandre J
AJ
Kioussis
Dimitris
D
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D017101:Bacteriophage P1 / Q000235:genetics; D018801:CD2 Antigens / Q000235:genetics; D019070:Cell Lineage; D018390:Gene Targeting / Q000379:methods*; D017930:Genes, Reporter; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D019426:Integrases / Q000096:biosynthesis* / Q000235:genetics; D019901:Locus Control Region; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008221:Lymphoid Tissue / Q000378:metabolism*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D015513:Oncogene Proteins / Q000235:genetics; D009928:Organ Specificity; D051567:Proto-Oncogene Proteins c-vav; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D013601:T-Lymphocytes / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
D001426:Bacterial Proteins; D018801:CD2 Antigens; D008164:Luminescent Proteins; D015513:Oncogene Proteins; D051567:Proto-Oncogene Proteins c-vav; D011993:Recombinant Fusion Proteins; C496618:VAV1 protein, human; C496619:Vav1 protein, mouse; D014764:Viral Proteins; C054562:yellow fluorescent protein, Bacteria; C045073:Cre recombinase; D019426:Integrases