[Cytokine expression in effusions of secretory otitis media].
OBJECTIVE
To assess the cytokine level in effusions of secretary otitis media and it's relationship with clinical index. To further study the inflammatory mechanism of otitis media with effusion.


METHODS
The concentration of TNF-alpha and TNFsolR II was quantitatively determined using enzyme-linked immunosorbent assay and it was correlated with clinical index in otitis media with effusion using stepwise regression analysis.


RESULTS
TNF-alpha and TNFsolR II was found in 100% effusions. Mean values of TNF-alpha were (3.42 +/- 2.25) ng/g total protein. Mean values of TNFsolR II were (405.80 +/- 216.53) ng/g total protein. Mean values of TNFsolR II index was (154.18 +/- 90.45) U. There was a significant decrease of TNFsolR II and TNFsolR II index in patients who recurred (P < 0.05).


CONCLUSIONS
Cytokine TNF-alpha and TNFsolR II are important inflammatory medium which exist in all otitis media effusions. The decrease of the level of TNFsolR II and TNFsolR II index present significantly in middle ear effusions with recurrence, but there is no any relation of TNF-alpha in middle ear effusions with recurrence.
Gao
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W
Department of Otorhinolaryngology, First Hospital, Beijing Medical University, Beijing 100034.
Liu
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S
Hao
H
H
D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010034:Otitis Media with Effusion / Q000378:metabolism*; D018124:Receptors, Tumor Necrosis Factor / Q000032:analysis* / Q000096:biosynthesis; D012008:Recurrence; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000096:biosynthesis
D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha