[Composition and structure of bioflocculant BP25].
A strain of Bacillus megaterium screened from activated sludge could produce exocellular flocculant. The flocculant was purified through ethanol precipitation and Sephadex S-500 column chromatography. The purified flocculant BP25 was assayed by Bradford reaction, agarose gel electrophoresis and Sulfate-phenol method. The results showed that BP25 was a kind of polysaccharide which contains 36.97% O, 6.28% H, 47.00% C, 0% No NMR assay showed BP-25 contains no uronic acid. Gas-chromatography assay combined with thin-layer chromatography of acid hydralate revealed that BP25 contains Glucose and Mannose with the mole ratio of 4:1. Methylation analysis revealed the polysaccharide contains alpha-1, 6 glycosidicbond and alpha-1, 3 glycosidicbond. The main chains are comprised of Glucose and all the Mannose are in the side chains. Possible repeating unit structure was deduced.
Liu
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Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Xu
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Liu
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Yang
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D001410:Bacillus megaterium / Q000737:chemistry* / Q000302:isolation & purification; D005427:Flocculation; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000032:analysis; D008358:Mannose / Q000032:analysis; D008745:Methylation; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry; D012722:Sewage / Q000382:microbiology
D011135:Polysaccharides, Bacterial; D012722:Sewage; D005947:Glucose; D008358:Mannose