The comprehensive mouse radiation hybrid map densely cross-referenced to the recombination map: a tool to support the sequence assemblies.
We have developed a unique comprehensive mouse radiation hybrid (RH) map of nearly 23,000 markers integrating data from three international genome centers and over 400 independent laboratories. We have cross-referenced this map to the 0.5-cM resolution recombination-based Jackson Laboratory (TJL) backcross panel map, building a complete set of RH framework chromosome maps based on a high density of known-ordered anchor markers. We have systematically typed markers to improve coverage and resolve discrepancies, and have reanalyzed data sets as needed. The cross-linking of the RH and recombination maps has resulted in a highly accurate genome-wide map with consistent marker order. We have compared these linked framework maps to the Ensemble mouse genome sequence assembly, and show that they are a useful medium resolution tool for both validating sequence assembly and elucidating chromosome biology.
Rowe
Lucy B
LB
The Jackson Laboratory, Bar Harbor, Maine 04609, USA. lbr@jax.org
Barter
Mary E
ME
Kelmenson
Jennifer A
JA
Eppig
Janan T
JT
D000818:Animals; D000835:Animals, Wild; D002875:Chromosomes / Q000235:genetics; D020451:Contig Mapping; D006224:Cricetinae; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics; D023061:Gene Order / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D021101:Radiation Hybrid Mapping / Q000379:methods*; D011995:Recombination, Genetic / Q000235:genetics*; D014960:X Chromosome / Q000235:genetics
D005819:Genetic Markers; D004247:DNA