title: [Study on the plasmid instability of Bdellovibrio BDG-9].
abstract: A new method system was established in this paper to study the plasmid in stability of Bdellovibrio BDG-9. Using this system, it was found that when BDG-9 was cultured singly on the SMB plate, plasmid pST I was unstable although pST I still replicated and distributed to progeny cell normally. The results showed that the pST I copy number in single cell of BDG-9 decreased gradually to zero with the propagation of BDG-9. Additionally, plasmid pST I was very important for the growth of BDG-9, and with the lacking of pST I s, the growth and propagation of BDG-9 ceased.
authors.affiliation: Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, 100084.
authors.forename: P
authors.initials: P
authors.lastname: Jiao
authors.forename: C
authors.initials: C
authors.lastname: Ma
authors.forename: S
authors.initials: S
authors.lastname: Wang
mesh_terms: D001501:Bdellovibrio / Q000235:genetics*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics*; D018628:Gene Dosage; D010957:Plasmids / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial