[Determination and purification of beta-carotene in Spirulina maximum].
There are several kinds of nutritious materials in Spirulina maximum, and beta-carotene is one of the most important materials. Because beta-carotene is of low polarity, for purification it was extracted with the mixture of acetone and methanol in the ratio of 7:2 (V/V), then saponified, and extracted with petroleum ether, purified with column packed with neutral Al2O3. Its content was determined by reversed-phase HPLC. This method is accurate and the results are reproducible. The average recovery was 96.8%.
Hu
Z H
ZH
Lab of Algal and Bacterial Technology, Department of Biological Science and Technology, Nanjing University, Nanjing 210093, China. hzhbio@263.net
Liu
Z L
ZL
D001426:Bacterial Proteins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D053545:Spirulina; D019207:beta Carotene / Q000032:analysis* / Q000302:isolation & purification
D001426:Bacterial Proteins; D019207:beta Carotene