[Cloning of trehalose-6-phosphate synthase gene from S. cerevisiae and its plant expression vector construction].
Total RNA, mRNA were isolated from baker's yeast S. cerevisiae, the cDNA was prepared with AMV Reverse Transcriptase from the total mRNA. The gene of trehalose-6-phosphate synthase was cloned from the cDNA with PCR amplification. The gene was sequenced and the results showed that the tpsl gene contains 1507 nucleotides and 99.6% identity with S. cerevisiae. The tps1 gene was constructed on the plant expression vector pBin438.
Chen
H
H
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Zhu
L
L
Dong
Z
Z
D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D015870:Gene Expression; D005800:Genes, Fungal; D005822:Genetic Vectors; D005964:Glucosyltransferases / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism
D004271:DNA, Fungal; D004274:DNA, Recombinant; D012333:RNA, Messenger; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase