title: Ectoprotein kinase-mediated phosphorylation of FAT/CD36 regulates palmitate uptake by human platelets.
abstract: Glycoprotein IV (FAT/CD36) has been shown to be phosphorylated by a cAMP-dependent, platelet membrane-bound ectokinase. In this study, we demonstrate that ectophosphorylation of FAT/CD36 regulates initial palmitate uptake. This is the first time that short-term regulation of the activity of a long-chain fatty acid carrier could be shown. Phosphorylation of FAT/CD36 was paralleled by a significant decrease in initial palmitate uptake by morphologically and functionally intact platelets. Maximum inhibition of palmitate uptake was achieved at 0.5 nM extracellular ATP, being significantly decreased to 72% compared to the control. Inhibition of palmitate uptake was abolished by co-incubation with the specific protein kinase A inhibitor peptide PKI or with beta,gamma-methylene-ATP, and was reversible upon addition of alkaline phosphatase. An extracellular ATP concentration above 5 microM completely prevented the ectophosphorylation-mediated inhibition of palmitate uptake. We conclude that FAT/CD36-mediated palmitate uptake by human platelets is short-term regulated via cAMP-dependent ectophosphorylation of FAT/CD36.
authors.affiliation: Klinik für Neonatologie, Charité-CCM, Humboldt-Universität zu Berlin, 10098 Berlin, Germany. florian.guthmann@charite.de
authors.forename: F
authors.initials: F
authors.lastname: Guthmann
authors.forename: P
authors.initials: P
authors.lastname: Maehl
authors.forename: J
authors.initials: J
authors.lastname: Preiss
authors.forename: I
authors.initials: I
authors.lastname: Kolleck
authors.forename: B
authors.initials: B
authors.lastname: Rüstow
mesh_terms: D001792:Blood Platelets / Q000378:metabolism*; D018955:CD36 Antigens / Q000378:metabolism*; D006801:Humans; D010168:Palmitates / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*
chemical_list: D018955:CD36 Antigens; D010168:Palmitates; D011494:Protein Kinases; C034454:ectoprotein kinase