title: [Determination of the point of gene mutation in two types of tissue from the aminoglycoside antibiotics induced deaf patients].
abstract: OBJECTIVE
  To elucidate the gene mutation presents in hair follicle cells, just as blood cells, in the aminoglycoside antibiotics induced deaf patients(AAID).
  
  
  METHODS
  Mitochondrial DNA of blood cells and/or hair follicle cells from 8 members of 3 aminoglycoside antibiotics induced deafness families were analysed using PCR-SSCP and Alw 26 I digestian.
  
  
  RESULTS
  Our result showed that a gene mutation at nucleotide 1555 in 12 S rRNA of mitochondrial DNA appeared in blood cells and/or hair follicle cells of 7 subjects among those 8 members. Only a father with normal hearing did't exhibit such a mutation.
  
  
  CONCLUSIONS
  This indicated that hairs could be used as a sample instead of blood to perform gene examination for AAID.
authors.affiliation: Department of Otorhinolaryngology, Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310009.
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: C
authors.initials: C
authors.lastname: Xing
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D004272:DNA, Mitochondrial / Q000235:genetics*; D003638:Deafness / Q000139:chemically induced / Q000235:genetics*; D005260:Female; D006198:Hair Cells, Auditory / Q000737:chemistry; D018859:Hair Follicle / Q000737:chemistry; D006801:Humans; D008297:Male; D010375:Pedigree; D017354:Point Mutation
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004272:DNA, Mitochondrial