TP53 codon 72 polymorphism in oral squamous cell carcinoma.
BACKGROUND
Oral squamous cell carcinoma (OSCC) is a multifactorial neoplasm related to tobacco smoke, alcohol consumption and molecular genetic changes such as p53 mutations. The human tumor suppressor gene TP53 contains single nucleotide polymorphism that encodes either arginin (Arg) or proline (Pro) at amino acid codon 72 of the p53 protein. The relationship between human cancer susceptibility and p53 polymorphism at codon 72 is controversial. The aim of this study was to investigate the association between this polymorphism and OSCC development in a Brazilian population.


METHODS
Eighty-two patients with OSCC and 82 age-sex-matched controls were included in the study. DNA was extracted from all subjects' normal oral mucosa and the polymerase chain reaction amplification (PCR) was performed for detection of the TP53 genotypes. PCR products were analyzed in a 6.5% polycrylamide gel and silver-stained. Statistical analyses were performed with the chi 2 test and Fisher's exact test.


RESULTS
The frequencies of the genotypes Arg/Arg, Arg/Pro and Pro/Pro, were 37.8%, 54.8% and 7.4%, respectively, for OSCC cases and 40.2%, 54.8% and 4.9% for controls. No significant differences in the distribution of TP53 genotypes were seen between the groups (p = 0.794).


CONCLUSIONS
The present study does not support the hypothesis that this TP53 polymorphism is associated with oral cancer susceptibility.
Drummond
Sérgio Neves
SN
Department of Oral Surgery and Pathology, Universidade Federal de Minas Gerais, Brazil.
De Marco
Luiz
L
Pordeus
Isabela De Almeida
Ide A
Barbosa
Alvimar Afonso
AA
Gomez
Ricardo Santiago
RS
D000483:Alleles; D001120:Arginine / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016022:Case-Control Studies; D003062:Codon / Q000235:genetics; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008297:Male; D009062:Mouth Neoplasms / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011392:Proline / Q000235:genetics; D012907:Smoking / Q000009:adverse effects / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
D003062:Codon; D016159:Tumor Suppressor Protein p53; D001120:Arginine; D011392:Proline