Identification and characterization of a new member of serpin family- HongrES1 in rat epididymis.
A full length cDNA named HongrES1 was isolated and cloned by screening rat epididymis cDNA library using a mouse EST as a probe and 5'RACE followed. It contained 1590bp nucleotides and its predicted protein had 415 amino acid residues including a serpin (serine protease inhibitor) conserved domain. Tissue distribution pattern showed it was specifically expressed in adult rat epididymis; moreover, in situ hybridyzation indicated this gene was expressed in a limited region of the cauda epididymis near vas deference. Such kind of expression pattern sugested that HongrES1 had potential function in male reproduction.
Hu
Zhi Hong
ZH
State Key Laboratory of Molecular Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Liu
Qiang
Q
Shang
Quan
Q
Zheng
Min
M
Yang
Jun
J
Zhang
Yong Lian
YL
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D001483:Base Sequence / Q000235:genetics; D002454:Cell Differentiation / Q000502:physiology*; D018076:DNA, Complementary / Q000032:analysis; D004822:Epididymis / Q000166:cytology / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012098:Reproduction / Q000502:physiology; D015843:Serpins / Q000235:genetics / Q000302:isolation & purification*; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*
D018076:DNA, Complementary; D012333:RNA, Messenger; C476408:Serpina16 protein, rat; D015843:Serpins