[Research of the extraction method of morphine from biological fluids].
A solid-phase extraction method of morphine from urine and blood has introduced. The effect of 5 SPE columns, 3 eluents and pH on morphine recovery has been investigated systematically. Derivative GC was used as a method of detection. The result showed that the column and the eluent of such as GDX-301, GDX-403 and C18 chloroform:isopropanol (9:1) had good behaviors to extraction of morphine. When GDX-301 was used as a sorbent, the recovery of morphine from urine was above 90% at pH 9, then went down with the increase of pH. While the recovery from blood was growing with the increase of pH, which reached above 90% in strong alkaline. The extraction method is simple, inexpensive, efficient and reproducible, which provides an effective and practical method to extract morphine and similar illicit drugs from biological fluids.
Meng
P J
PJ
Chinese Criminal Police College, Shenyang 110035, P. R. China.
Wang
J H
JH
Jiang
Z L
ZL
Yao
L J
LJ
Wang
Y J
YJ
D002849:Chromatography, Gas; D006801:Humans; D007202:Indicators and Reagents; D009020:Morphine / Q000097:blood* / Q000652:urine*; D015813:Substance Abuse Detection / Q000379:methods*
D007202:Indicators and Reagents; D009020:Morphine