Molecular biological characterization of equine surfactant protein A.
In the following, we describe the isolation and sequencing of the equine surfactant protein A (Sp-A) as found in both the cDNA and the genomic DNA. We found a length of the cDNA sequence of 747 bp (base pairs), in translation into amino acids of 248. Compared with the known molecular biological facts about Sp-A in other species, the cDNA sequence obtained showed highest homology with that of sheep (85.01%). The genomic DNA of equine Sp-A, as in other species, includes three introns. There were no hints for the existence of two different Sp-A genes. These results should form the basis for a better understanding of respiratory failure in foals and adult horses, and also lead to further studies on this item.
Hospes
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R
Interdisciplinary Research Team on Perinatology/Neonatology Justus-Liebig-University, Giessen, Germany. rainer.hospes@vetmed.uni-giessen.de
Hospes
B I L
BI
Reiss
I
I
Bostedt
H
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Gortner
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L
D000818:Animals; D017931:DNA Primers; D006734:Horse Diseases / Q000235:genetics; D006736:Horses / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics*; D012131:Respiratory Insufficiency / Q000235:genetics / Q000662:veterinary
D017931:DNA Primers; D037662:Pulmonary Surfactant-Associated Protein A