title: Phenotypic switching of in vitro mandibular condylar cartilage during matrix mineralization.
abstract: In order to analyze the phenotypic conversion of chondrocytes, mandibular condyles of mice and rabbits were cultured under cell and organ culture systems, and then examined by a combination of morphological and biochemical procedures. In organ culture, mandibular condylar cartilage (MCC) obtained from newborn mice began to mineralize from the central zone and then progressively widened towards the peripheral zone. Electron microscopic observations showed that with the increasing duration of the organ culture, chondrocytes at the central zone converted into spindle-shaped osteoblastic cells accompanying the formation of the bone type of thick-banded collagen fibrils. To obtain a better understanding of the chondrocytic conversion, immunolocalizations for type I and type X collagens and osteocalcin (OC) were examined in mouse MCC cells in cell culture. Type X collagen and OC were expressed almost simultaneously at the late stage of culture, and type I collagen was detected along the calcified nodules after the production of these proteins. Northern blot analysis in cell cultures of rabbit MCC indicated that type II collagen and alkaline phosphatase (ALPase) messenger ribonucleic acids (mRNAs) were highly expressed at day 7, but subsequently decreased. In contrast, mRNA for type I collagen was expressed at a low level on day 7 and peaked on day 12. The present results suggest that, morphologically and biochemically, cellular modification in MCC cells under culture conditions occurs at a cellular morphological level and also at marker-gene-expression level.
authors.affiliation: Department of Molecular Interaction and Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Inoue
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hiraki
authors.forename: Tokio
authors.initials: T
authors.lastname: Nawa
authors.forename: Kiyoto
authors.initials: K
authors.lastname: Ishizeki
mesh_terms: D000375:Aging / Q000235:genetics / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism* / Q000648:ultrastructure; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D024121:Collagen Type X / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism* / Q000648:ultrastructure; D005333:Fetus; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D008334:Mandible / Q000196:embryology* / Q000254:growth & development / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D009924:Organ Culture Techniques; D010006:Osteoblasts / Q000378:metabolism* / Q000648:ultrastructure; D010012:Osteogenesis / Q000235:genetics*; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits
chemical_list: D024042:Collagen Type I; D024043:Collagen Type II; D024121:Collagen Type X; D012333:RNA, Messenger; D000469:Alkaline Phosphatase