title: Long-chain PUFA supplementation improves PUFA profile in infants with cholestasis.
abstract: Long-chain PUFA (LCP) deficiency is a frequent complication in cholestatic infants. We investigated the effects of LCP-supplemented formula on EFA status in infants with cholestasis. Twenty-three infants with cholestasis (biliary atresia after surgery, 8; intrahepatic cholestasis, 15) aged 1.9 to 4.9 mon (median 3.1 mon) were randomized to receive commercial infant formulas either without LCP or with LCP from egg phospholipids for 1 mon. Liver tests, nutrient intakes, and plasma phospholipid FA (%w/w) were determined at baseline and after intervention. At baseline, patients had high serum direct bilirubin levels (5.9 +/- 3.0 mg/dL; mean +/- SD), they were malnourished (body fat mass: 40 +/- 13% of normal) and presented with PUFA deficiency [plasma phospholipid PUFA: 28.43%w/w (26.56-30.53) in patients vs. 37.02%w/w (34.53-39.58) in controls; median (1st-3rd quartile)] with elevated Mead acid and palmitoleic acid. LCP-supplemented (n = 11) and -nonsupplemented groups (n = 12) did not differ in age, indicators of liver function, and EFA status at baseline. After the intervention, LCP-supplemented infants had higher levels of arachidonic acid [7.2 (5.9-8.8) vs. 4.2 (3.0-5.3) %w/w; P < 0.001] and DHA [2.8 (2.2-3.2) vs. 1.6 (1.0-2.1) %w/w; P < 0.05], accompanied by increased TBARS concentration: 1.9 (1.4-2.2) vs. 1.3 (1.1-1.6) nmol/mL; P < 0.05]. We concluded that LCP-supplemented formulae improve LCP status of infants with severe cholestasis but may enhance lipid peroxidation.
authors.affiliation: Department of Gastroenterology, Hepatology and Nutrition, The Children's Memorial Health Institute, Al. Dzieci Polskich 20, 04-736 Warszawa, Poland, sochap@czd.waw.pl
authors.forename: Piotr
authors.initials: P
authors.lastname: Socha
authors.forename: Berthold
authors.initials: B
authors.lastname: Koletzko
authors.forename: Irena
authors.initials: I
authors.lastname: Jankowska
authors.forename: Joanna
authors.initials: J
authors.lastname: Pawłowska
authors.forename: Hans
authors.initials: H
authors.lastname: Demmelmair
authors.forename: Anna
authors.initials: A
authors.lastname: Stolarczyk
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Swiatkowska
authors.forename: Jerzy
authors.initials: J
authors.lastname: Socha
mesh_terms: D001647:Bile Acids and Salts / Q000097:blood; D001663:Bilirubin / Q000097:blood; D001827:Body Height; D001835:Body Weight; D001942:Breast Feeding; D002779:Cholestasis / Q000097:blood* / Q000150:complications*; D019587:Dietary Supplements; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage / Q000097:blood* / Q000172:deficiency / Q000494:pharmacology*; D006801:Humans; D007223:Infant; D008099:Liver / Q000378:metabolism; D009752:Nutritional Status; D044465:White People
chemical_list: D001647:Bile Acids and Salts; D005231:Fatty Acids, Unsaturated; D001663:Bilirubin