[A brief introduction to the methods for novel gene cloning].
There are a lot of methods for novel gene cloning, but how to clone candidate gene(s) quickly and correctly? This is a brief introduction to methods of novel gene cloning, these methods includes: differential display reverse transcriptase polymerase chain reaction(DD RT-PCR), suppression subtractive hybridization(SSH), RNA arbitrarily primed PCR(RAP-PCR), representational difference analysis(RDA), yeast two-hybrid system, cDNA capturation, et al. We not only introduced these methods, but also discussed the advantages and disadvantages of them. However, no single method is omnipotent, one should pick up the method most suitable for a special purpose.
Sun
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Laboratory of Neuronal Injury and Regeneration, Shanghai Research Center of Life Sciences, Chinese Academy of Sciences, Shanghai 200031.
Yu
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D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005818:Genetic Engineering; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D019105:Random Amplified Polymorphic DNA Technique; D011995:Recombination, Genetic
D004247:DNA