title: Single multiplex polymerase chain reaction to detect diverse loci associated with diarrheagenic Escherichia coli.
abstract: We developed and tested a single multiplex polymerase chain reaction (PCR) that detects enterotoxigenic, enteropathogenic, enteroinvasive, and Shiga toxin-producing Escherichia coli. This PCR is specific, sensitive, and rapid in detecting target isolates in stool and food. Because of its simplicity, economy, and efficiency, this protocol warrants further evaluation in large, prospective studies of polymicrobial substances.
authors.affiliation: Dpto. de Biomedicina Molecular, CINVESTAV-IPN, México D.F., México.
authors.forename: Catalina
authors.initials: C
authors.lastname: López-Saucedo
authors.forename: Jorge F
authors.initials: JF
authors.lastname: Cerna
authors.forename: Nicolas
authors.initials: N
authors.lastname: Villegas-Sepulveda
authors.forename: Rocío
authors.initials: R
authors.lastname: Thompson
authors.forename: F Raul
authors.initials: FR
authors.lastname: Velazquez
authors.forename: Javier
authors.initials: J
authors.lastname: Torres
authors.forename: Phillip I
authors.initials: PI
authors.lastname: Tarr
authors.forename: Teresa
authors.initials: T
authors.lastname: Estrada-García
mesh_terms: D017931:DNA Primers; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D029968:Escherichia coli Proteins