Enzymic preparation of water-soluble chitosan and their antitumor activity.
Water-soluble low-molecular-weight (LMW) chitosan was prepared from enzymatic hydrolysis with efficient hemicellulase. The hydrolysates were separated by ultrafiltration membranes. A separated fraction with Mw more than 5x10(3) and with a degree of deacetylation of 58% was water-soluble in the free amine form. The intraperitoneal injection of LMW chitosan and its N-acetyl product inhibited the growth of sacroma 180 (S180) tumor cells in the mice, and the maximum inhibitory rate reached 64.2%. The oral administration was also effective on decreasing weight of tumor, and the maximum inhibitory rate reached 33.7%. The Water-soluble chitosan with higher Mw than hexamer might have better antitumor activity.
Qin
Caiqin
C
Department of Environmental Science, Wuhan University, 430072, Wuhan, PR China.
Du
Yumin
Y
Xiao
Ling
L
Li
Zhan
Z
Gao
Xiaohai
X
D000107:Acetylation; D000284:Administration, Oral; D000588:Amines / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000494:pharmacology*; D048271:Chitosan; D005260:Female; D006026:Glycoside Hydrolases / Q000737:chemistry; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D013055:Spectrophotometry, Infrared; D013696:Temperature; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000588:Amines; D000970:Antineoplastic Agents; D002686:Chitin; D048271:Chitosan; D006026:Glycoside Hydrolases; C023305:hemicellulase