Structural characterization of the O-chain polysaccharide isolated from Bordetella avium ATCC 5086: variation on a theme(1).
The O-chain polysaccharide (O-PS) of Bordetella avium was isolated from the lipopolysaccharide by mild acid hydrolysis to remove the lipid A, followed by hydrofluorolysis to remove the lipopolysaccharide core oligosaccharide leaving a residual O-PS for structural analysis. High resolution (1)H and (13)C NMR and MALDI studies showed the O-chain to be a polymer composed of 1,4-linked 2-acetamidino-3-[3-hydroxybutanamido]-2,3-dideoxy-beta-D-glucopyranosyluronic acid residues.
Larocque
Suzon
S
Institute for Biological Sciences, NRC, Ottawa, ON, Canada.
Brisson
Jean-Robert
JR
Thérisod
Hélène
H
Perry
Malcolm B
MB
Caroff
Martine
M
D020155:3-Hydroxybutyric Acid / Q000737:chemistry; D000081:Acetamides / Q000737:chemistry; D001884:Bordetella / Q000737:chemistry*; D002247:Carbon Isotopes; D004591:Electrophoresis, Polyacrylamide Gel; D006858:Hydrofluoric Acid / Q000737:chemistry; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000081:Acetamides; D002247:Carbon Isotopes; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; C030686:acetamide; D006858:Hydrofluoric Acid; D020155:3-Hydroxybutyric Acid