Immunohistochemical investigations of neurofilament M' and alphabeta-crystallin in the magnocellular layers of the primate lateral geniculate nucleus.
The magnocellular and parvocellular pathways are two major processing streams in the primate visual system. Using high-density grid arrayed cDNA clones to hybridize to cDNA probes from cortical regions of each pathway, a list of candidate differentially expressed genes was produced [Mol. Brain Res. 82 (2000) 11-24]. Magnocellular pathway candidates include neurofilament M' and alphabeta-crystallin. Using antibodies generated against these proteins, immunohistochemical analysis revealed preferential staining of the magnocellular layers in the primate lateral geniculate nucleus, providing verification of two candidate magnocellular-enriched genes.
Prasad
S S
SS
Department of Ophthalmology and Visual Sciences, University of British Columbia, 2550 Willow Street, Vancouver, British Columbia, Canada V5Z 3N9.
Schnerch
A
A
Lam
D Y
DY
To
E
E
Jim
J
J
Kaufman
P L
PL
Matsubara
J A
JA
D000818:Animals; D005829:Geniculate Bodies / Q000378:metabolism*; D007150:Immunohistochemistry; D008252:Macaca fascicularis; D008253:Macaca mulatta; D016900:Neurofilament Proteins / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D014795:Visual Pathways / Q000378:metabolism; D038203:alpha-Crystallin B Chain / Q000378:metabolism*
D016900:Neurofilament Proteins; D038203:alpha-Crystallin B Chain; C061195:neurofilament protein M