[A study on the biological properties of fimbriae of Actinomyces viscosus. I. The preparation and determination of fimbriae of A. viscosus].
OBJECTIVE
To build up the methods to prepare and determine the fimbriae of A. viscosus.


METHODS
Type 1 and 2 fimbriae were prepared from A. viscosus 5519 and 5951 respectively. Fimbriae were isolated from bacteria cells by mechanical shaking, obtained by collecting the supernatant after centrifugation, and purified by ammonium sulfate precipitations and gel filtration.


RESULTS
Electron microscope showed that fimbriae existed in the preparations. The immunological reaction between the fimbriae preparations and specific antibodies against fimbriae attested that they were type 1 or type 2 fimbriae.


CONCLUSIONS
The present study become a foundation for further study on the biological properties of fimbriae of A. viscosus.
Li
W
W
College of Stomatology, West China University of Medical Sciences.
Liu
T
T
Zhang
C
C
Yue
S
S
Zhao
C
C
D016924:Actinomyces viscosus / Q000302:isolation & purification*; D010861:Fimbriae, Bacterial