[Expression of otsA gene in tobacco and improvement stress tolerance].
The Escherichia coli trehalose-6-phosphate synthase gene(otsA) was engineered under the control of cauliflower mosaic virus regulatory sequences (CaMV35S) for expression in plants. OtsA gene was incorporated into the chromosome DNA by Agrobacterium-mediated transfer and expressed in Nicotiana tabacum. The otsA gene transgenic plant exhibited multiple phenotypic alteration: improved stunted growth and drought tolerance. The detached leaves from the transgenic plant, their water loss slower than that of the controls. Trehalose accumulated in transgenic plant was determined by HPLC suggest that synthesis of the sugar improving plant to stress tolerance.
Dai
X
X
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Wang
Y
Y
Yang
B
B
Zhou
J
J
D015870:Gene Expression; D005818:Genetic Engineering; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology*; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics / Q000502:physiology*; D014199:Trehalose / Q000378:metabolism
D014199:Trehalose; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase