[Studies on liver cancer induced by non-necrotizing dose of diethylnitrosamine in rats].
OBJECTIVE
To provide an appropriate animal model of liver cancer and sensitive indicator for carcinogen risk assessment.


METHODS
The developmental processes of liver cancers in rats initiated by non-necrotizing dose of diethylnitrosamine and promoted by phenobarbital were studied.


RESULTS
Most of the preneoplastic lesions and liver cancers were made up of eosinophilic cells. The placental glutathione S-transferase (P-GST) staining could allow a lot of positive single cells, foci and nodules to be seen. But the lesions showed by HE staining expressed higher proliferating cell nuclear antigen (PCNA) than those showed by P-GST staining expressed, indicating a greater possibility for these lesions to persist and develop further. The cellular morphologic analysis demonstrated that the ratio of nuclear radii to cytoplasm radii decreased in the cell in PCNA positive expressing nodules. In addition, the nodules showed by P-GST expressed higher PCNA than those the foci expressed, indicating an increased possibility to develop further in the nodules.


CONCLUSIONS
PCNA expression may be used as a relatively ideal marker for predicting the potential development of precancerous lesions in the experimental model of rat liver cancer induction.
Jiang
Y
Y
Department of Toxicology, School of Public Health, WCUMS, Chengdu 610041, China.
Dong
Q
Q
Xiao
B
B
Li
S
S
D000818:Animals; D004052:Diethylnitrosamine; D004195:Disease Models, Animal; D005982:Glutathione Transferase / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D018809:Proliferating Cell Nuclear Antigen; D004052:Diethylnitrosamine; D005982:Glutathione Transferase