[An experimental study on traumatic brain injury for inducing neuronal apoptosis in rats].
This study was designed to explore the effect of traumatic brain injury and its mechanism for inducing neuronal apoptosis. The model of experimental traumatic brain injury was used. The rats were dispatched at different times(3 h, 12 h, 24 h, 48 h, 72 h) after traumatic brain injury, and the neuronal apoptosis was evaluated with microscope (HE staining), TUNEL method, flow cytometry, gel electrophoresis and immunohistochemistry assay. The results showed that the brain tissue neurons treated by traumatic brain injury underwent morphological changes of apoptosis, the DNA presented "ladder" break, the rate of neuronal apoptosis at different times ranged from 9.8% to 14.0%, but that of the control group was 1.7% and the expressions of related apoptosis genes(c-myc, fas and fasL) were increased. The findings of this study indicate that traumatic brain injury can induce neuronal apoptosis, and its molecular mechanism might be related to the expression of some apoptosis genes.
Jiang
S
S
Department of Neurosurgery, First Affiliated Hospital, WCUMS, Chengdu 610041.
Yu
R
R
Mao
B
B
Gao
L
L
D000818:Animals; D017209:Apoptosis; D001930:Brain Injuries / Q000235:genetics / Q000473:pathology*; D005434:Flow Cytometry; D015870:Gene Expression; D016259:Genes, myc; D008297:Male; D009474:Neurons / Q000473:pathology; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D016271:Proto-Oncogene Proteins c-myc