[Expression of VEGF on rabbit skin fibroblasts using retroviral vector].
A replication-deficient recombinant retrovirus containing the cDNA coding for human vascular endothelial growth factor (VEGF) was generated, and then infected rabbit primary skin fibroblasts. After selection with G418, the transduced colonies have the ability of producing VEGF. The integration and expression of VEGF in transduced cells were confirmed by Southern blot, PCR, Northern blot and RT-PCR assay. The VEGF secreted by transduced cells has strong bioactivity when assayed by endothelial proliferation and Miles vascular permeability assay. Thus, this study pave the way for future study of biological and physiological effect of VEGF in vivo.
He
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Research Center for Human Gene Therapy, Shanghai Second Medical University, Shanghai 200025.
Shi
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Chen
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D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D011817:Rabbits; D012867:Skin / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000235:genetics*
D018076:DNA, Complementary; D042461:Vascular Endothelial Growth Factor A