title: [Study on the production of trehalose by bacterium D-97 endocellular enzymes using HPLC/RI and HPLC/ESI-MS].
abstract: The mechanism in which trehalose is produced from dextrin or starch hydrolyzate by endocellular enzymes of bacterium D-97 can be elucidated high performance liquid chromatography (HPLC) with differential refraction detection (RI) basically, including the effect of the different carbon sources on the endocellular trehalose-producing enzymes in bacterium D-97 and the possibility or ability of the endocellular enzymes to produce trehalose using maltooligosaccharides of different chain lengths. After purification of endocellular enzymes of bacterium D-97, two enzymes (called Enzyme A and Enzyme B) related to trehalose synthesis were found. The unknown oligosaccharides produced by Enzyme A were analyzed with the HPLC/RI and HPLC/ESI-MS (electrospray ionization mass spectrometry). The results showed the relative molecular masses of the unknown oligosaccharides were the same as those of the enzymatic reaction substances (maltotriose, maltotetraose and maltopentaose) respectively. In combining with other results of biological experiments, these unknown oligosaccharides had been identified basically. There was no reduction power in these unknown oligosaccharides and only one trehalose residue exited in the molecular chain of these unknown oligosaccharides.
authors.affiliation: School of Biotechnology, Southern Yangzte University, Wuxi 214036, China. rongshaofeng@yahoo.com
authors.forename: Shao-feng
authors.initials: SF
authors.lastname: Rong
authors.forename: Hai-ping
authors.initials: HP
authors.lastname: Zhang
authors.forename: Zuo-ying
authors.initials: ZY
authors.lastname: Duan
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Zhong-gui
authors.initials: ZG
authors.lastname: Mao
authors.forename: Jun
authors.initials: J
authors.lastname: Dai
mesh_terms: D001419:Bacteria / Q000201:enzymology / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013057:Spectrum Analysis / Q000379:methods; D014199:Trehalose / Q000096:biosynthesis*
chemical_list: D009844:Oligosaccharides; C019193:maltopentaose; D014199:Trehalose