title: Stepwise surface regeneration of electrochemical immunosensors working on biocatalyzed precipitation.
abstract: A new strategy of stepwise surface regeneration for electrochemical immunosensors, working on a biocatalyzed precipitation reaction, has been developed. The strategy is based on the combination of deposited product thin-film dissolution and bound-protein displacement reactions from the modified sensor surfaces. As a model system, surfaces functionalized with biotin groups and their affinity recognition/ displacement reactions with antibiotin antibody molecules were chosen and investigated for affinity-sensing and stepwise regeneration reactions.
authors.affiliation: BioMEMS Group, Basic Research Laboratory, ETRI, Gajeong-dong, Yuseong-gu, Daejeon 305-350, Korea. hcyoon@etri.re.kr
authors.forename: Hyun C
authors.initials: HC
authors.lastname: Yoon
authors.forename: Jong Soo
authors.initials: JS
authors.lastname: Ko
authors.forename: Haesik
authors.initials: H
authors.lastname: Yang
authors.forename: Youn Tae
authors.initials: YT
authors.lastname: Kim
mesh_terms: D000906:Antibodies / Q000032:analysis; D000915:Antibody Affinity; D015374:Biosensing Techniques; D001710:Biotin / Q000276:immunology
chemical_list: D000906:Antibodies; D001710:Biotin