Notch-1 induction, a novel activity of SV40 required for growth of SV40-transformed human mesothelial cells.
We show that SV40 infection of human mesothelial cells directly causes overexpression of Notch-1, a key cell regulatory gene. Notch-1 induction is achieved at the transcriptional level and requires both the SV40 large T-antigen and the small t-antigen. Notch-1 upregulation is maintained in SV40-transformed human mesothelial clones and in SV40-positive mesotheliomas and derived cell lines. Activation of Notch-1 promotes cell cycle progression and it is required for the growth of SV40-transformed mesothelial cells. Our finding is relevant to the process of SV40-mediated human cell transformation, an effect that cannot be accounted for solely by SV40-Tag inhibition of Rb and p53.
Bocchetta
Maurizio
M
Department of Pathology, Cardinal Bernardin Cancer Center, Loyola University Chicago, Maywood, IL 60153, USA.
Miele
Lucio
L
Pass
Harvey I
HI
Carbone
Michele
M
D002461:Cell Line, Transformed; D004848:Epithelium / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D008654:Mesothelioma / Q000378:metabolism / Q000473:pathology / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics; D051881:Receptor, Notch1; D011956:Receptors, Cell Surface; D013539:Simian virus 40 / Q000502:physiology*; D014157:Transcription Factors; D015854:Up-Regulation
D008565:Membrane Proteins; C497761:NOTCH1 protein, human; D012333:RNA, Messenger; D051881:Receptor, Notch1; D011956:Receptors, Cell Surface; D014157:Transcription Factors