Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma.
An analytic strategy was followed to identify putative regulatory genes during the development of human hepatocellular carcinoma (HCC). This strategy employed a bioinformatics analysis that used a database search to identify genes, which are differentially expressed in human HCC and are also under cell cycle regulation. A novel cell cycle regulated gene (HURP) that is overexpressed in HCC was identified. Full-length cDNAs encoding the human and mouse HURP genes were isolated. They share 72 and 61% identity at the nucleotide level and amino-acid level, respectively. Endogenous levels of HURP mRNA were found to be tightly regulated during cell cycle progression as illustrated by its elevated expression in the G(2)/M phase of synchronized HeLa cells and in regenerating mouse liver after partial hepatectomy. Immunofluorescence studies revealed that hepatoma up-regulated protein (HURP) localizes to the spindle poles during mitosis. Overexpression of HURP in 293T cells resulted in an enhanced cell growth at low serum levels and at polyhema-based, anchorage-independent growth assay. Taken together, these results strongly suggest that HURP is a potential novel cell cycle regulator that may play a role in the carcinogenesis of human cancer cells.
Tsou
Ann-Ping
AP
Institute of Biotechnology in Medicine, National Yang-Ming University, Taipei, Taiwan.
Yang
Chu-Wen
CW
Huang
Chi-Ying F
CY
Yu
Ricky Chang-Tze
RC
Lee
Yuan-Chii G
YC
Chang
Cha-Wei
CW
Chen
Bo-Rue
BR
Chung
Yu-Fang
YF
Fann
Ming-Ji
MJ
Chi
Chin-Wen
CW
Chiu
Jen-Hwey
JH
Chou
Chen-Kung
CK
D000595:Amino Acid Sequence; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics*; D003001:Cloning, Molecular; D030561:Databases, Nucleic Acid; D020224:Expressed Sequence Tags; D008099:Liver / Q000196:embryology / Q000502:physiology; D008113:Liver Neoplasms / Q000235:genetics*; D008115:Liver Regeneration / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008938:Mitosis; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism*; D009928:Organ Specificity; D017386:Sequence Homology, Amino Acid; D008941:Spindle Apparatus / Q000235:genetics
C465041:DLGAP5 protein, human; D009363:Neoplasm Proteins