[IL-1 beta mRNA and TNF-alpha mRNA expression in gingival tissues of patients with adult periodontitis].
OBJECTIVE
The aim of this study was to examine interleukin-1 beta (IL-1 beta) mRNA and tumor necrosis factor alpha (TNF-alpha) mRNA expression in gingival tissues of patients with adult periodontitis.


METHODS
A total of 19 patients, including 10 patients with adult periodontitis and 9 healthy individuals as the control, were evaluated for were selected for evaluating IL-1 beta mRNA and TNF-alpha mRNA expression, using reverse transcription polymerase chain reaction (RT-PCR) technique.


RESULTS
The relative amount of IL-1 beta mRNA expression of gingival samples from patients with adult periodontitis (0.819 +/- 0.045) (P < 0.01) was much higher than that from the normal control (0.306 +/- 0.087) (P < 0.01). The relative amount of TNF-alpha mRNA expression, gingival samples from AP patients (0.696 +/- 0.098) was also much higher than that from the normal control (0) (P < 0.01).


CONCLUSIONS
As the inflammatory and bone absorption mediators, IL-1 beta and TNF-alpha might play important roles in the pathogenesis and development of adult periodontitis.
Yin
L
L
College of Stomatology, China Medical University.
Li
L
L
Pan
Y
Y
Tan
Y
Y
He
A
A
D000293:Adolescent; D000328:Adult; D005881:Gingiva / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D010518:Periodontitis / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics
D007375:Interleukin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha