P2P-R deficiency modifies nocodazole-induced mitotic arrest and UV-induced apoptosis.
BACKGROUND
Cell cycle progression from G1 through S to mitosis can be influenced by microtubule-dependent mechanisms that involve AP1 factors and c-Jun N-terminal kinase (JNK) activity. UV irradiation-induced apoptosis also involves AP1 factors and JNK activity. The current studies evaluated the outcome of P2P-R deficiency on these mechanisms because P2P-R expression is repressed in association with a decrease in AP1 inducibility and cell cycle progression during differentiation, and P2P-R overexpression promotes apoptosis.


METHODS
The ability of the microtubule disruption drug nocodazole to induce mitotic arrest and the ability of UV irradiation to induce apoptosis was evaluated in native versus cells made P2P-R deficient by P2P-R antisense treatment.


RESULTS
P2P-R deficiency restricts cell cycle progression from G1 through S to mitosis in a microtubule-dependent manner and P2P-R deficiency represses UV irradiation-induced apoptosis.


CONCLUSIONS
P2P-R may influence AP1 and/or JNK signaling pathways.
Scott
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Department of Pathology, University of Tennessee Health Science Center, 3 North Dunlap Street, Memphis, TN 38163, USA. rescott@utmem.edu
Gao
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D016475:3T3 Cells; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects*; D002352:Carrier Proteins; D002455:Cell Division / Q000502:physiology; D016373:DNA, Antisense / Q000008:administration & dosage / Q000235:genetics; D048031:JNK Mitogen-Activated Protein Kinases; D051379:Mice; D008870:Microtubules / Q000187:drug effects / Q000502:physiology; D020928:Mitogen-Activated Protein Kinases / Q000502:physiology; D008938:Mitosis / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D015739:Nocodazole / Q000494:pharmacology*; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D018808:Transcription Factor AP-1 / Q000502:physiology; D014162:Transfection; D014466:Ultraviolet Rays
D000970:Antineoplastic Agents; D002352:Carrier Proteins; D016373:DNA, Antisense; D016601:RNA-Binding Proteins; C491290:Rbbp6 protein, mouse; D018808:Transcription Factor AP-1; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D015739:Nocodazole