Effects of glucocorticoids on leptin levels and eating behaviour in women.
OBJECTIVE
Long-term treatment with glucocorticoids induces weight gain and increased risk to develop obesity-related metabolic complications. The underlying mechanisms are not fully understood. Glucocorticoid therapy has previously been associated with increased levels of circulating leptin. In this study the eating behaviour was therefore studied in relation to leptin levels before and after short-term prednisolone treatment.


METHODS
Within-subject design.


METHODS
Twelve healthy postmenopausal women with a mean body mass index (BMI) of 28.9 kg m-2 (+/-0.8 SEM) volunteered after recruitment by an advertisement in the local paper.


METHODS
The subjects received 25 mg prednisolone daily for 7 days.


METHODS
Fasting serum samples were obtained before, during and after treatment for determination of leptin and insulin, glucose and fractionated lipoproteins in plasma. The microstructure of the eating behaviour was registered with a universal eating monitor, VIKTOR. Appetite was estimated by visual analogue rating scales and food intake by a 48-h recall.


RESULTS
Serum leptin increased after 2 and 7 days of glucocorticoid administration (P < 0.01), and the food intake measured by VIKTOR after 7 days of treatment (P < 0.05). No statistically significant changes were however, found in the 48-h food- recall or in the subjective appetite registrations. Insulin levels were borderline elevated (P = 0.062) after treatment, but no significant changes of fasting glucose were seen. High density lipoprotein cholesterol (HDL) increased (P < 0.05), whilst low density lipoprotein cholesterol (LDL) decreased (P < 0.05).


CONCLUSIONS
Food intake was elevated after glucocorticoid administration as observed with an objective, quantitative method, in spite of increased levels of circulating leptin.
Uddén
J
J
Centre for Metabolism and Endocrinology, Obesity Unit M73, Karolinska Institute at Huddinge University Hospital, 141 86 Stockholm, Sweden. joanna.udden@medhs.ki.se
Björntorp
P
P
Arner
P
P
Barkeling
B
B
Meurling
L
L
Rössner
S
S
D015992:Body Mass Index; D005215:Fasting / Q000097:blood; D005247:Feeding Behavior / Q000187:drug effects*; D005260:Female; D005938:Glucocorticoids / Q000009:adverse effects*; D006801:Humans; D020738:Leptin / Q000378:metabolism*; D008875:Middle Aged; D017698:Postmenopause; D011239:Prednisolone / Q000009:adverse effects*; D012307:Risk Factors; D015430:Weight Gain / Q000187:drug effects
D005938:Glucocorticoids; D020738:Leptin; D011239:Prednisolone