[Experimental studies on exons 5-8 of p53 gene mutation in laryngeal squamous cell carcinoma].
This study was designed to detect the point mutations of exons 5-8 of p53 gene in laryngeal squamous cell carcinoma (LSCC) and analyze their relationship. The detection of fresh tumor samples from LSCC patients was performed using silver staining PCR-SSCP method. From among 60 patients samples, 47 were positive in SSCP. Mutation rate was 78.3% (47/60). The results showed that the prevalence of p53 mutations in LSCC subjected to silver staining PCR-SSCP test were 50% (30/60) in exon 5, 11.67%(7/60) in exon 6, 41.6%(25/60) in exon 7, and 25%(15/60) in exon 8. The majority of the mutations were found in exon 5 and exon 7. Exon 5 and exon 7 of p53 gene may be the mutation hotspot in LSCC; they may be the critical position easily attacked by some carcinogen factors relating to LSCC.
Zhang
X
X
Department of Otorhinolaryngology, Fourth Affiliated Hospital, WCUMS, Chengdu 610041.
Liu
S
S
Zhou
G
G
Liang
C
C
Lin
D
D
Qiao
X
X
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D005091:Exons; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
D016159:Tumor Suppressor Protein p53