title: Single human leukocyte antigen flow cytometry beads for accurate identification of human leukocyte antigen antibody specificities.
abstract: BACKGROUND
  It is difficult to assign antibody specificity for highly sensitized patients using a cell panel with multiple antigens per reaction. We describe here a single antigen bead panel for accurate identification of human leukocyte antigen (HLA) antibody specificities by flow cytometry.
  
  
  METHODS
  A total of 110 single recombinant HLAs, including 34 A locus alleles, 57 B locus alleles, and 19 C locus alleles, were produced by a mammalian expression system. These single antigens were coated onto eight different colored microbeads, which were mixed together in one tube for simultaneous detection of HLA antibodies against eight different antigens per flow cytometry test.
  
  
  RESULTS
  Single HLA reacted specifically with the serologically defined monoclonal antibodies. The single antigen panel provided higher resolution than the regular cell panel for antibody detection by uncovering the masked specificities. Single antigens also provided higher sensitivity than the multiple antigens coated onto beads for HLA antibody detection as demonstrated by serum dilution studies. In 10 sera from patients who had rejected a kidney transplant, single antigen beads identified antibodies to 31 of 35 antigens that were mismatched in the donor. Most important, none of the reactions were against antigens present in the recipient.
  
  
  CONCLUSIONS
  An accurate and sensitive HLA antibody detection method is described using flow cytometry beads coated with single HLAs produced by recombinant technology. The single antigen beads should be useful in predicting negative crossmatch in highly sensitized organ recipients and highly sensitized patients requiring platelets.
authors.affiliation: Research Department, One Lambda. Inc., Canoga Park, CA, USA. rpei@onelambda.com
authors.forename: Rui
authors.initials: R
authors.lastname: Pei
authors.forename: Jar-How
authors.initials: JH
authors.lastname: Lee
authors.forename: Neng-Jen
authors.initials: NJ
authors.lastname: Shih
authors.forename: Mike
authors.initials: M
authors.lastname: Chen
authors.forename: Paul I
authors.initials: PI
authors.lastname: Terasaki
mesh_terms: D000918:Antibody Specificity; D005434:Flow Cytometry / Q000379:methods*; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000097:blood* / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D015395:Histocompatibility Antigens Class I; D007518:Isoantibodies; D011994:Recombinant Proteins