[The epoxidation of cis-propenylphophonic acid to fosfomycin by Pencillium sp].
One strain of Penicillium sp. F5, found from soil sample, was able to stereo-specific epoxidation of cis-propenylphosphonic acid(PPOH) to fosfomycin[(-)-(1R, 2S)-1, 2-epoxypropylphosphonic acid, FOM] during the cultivation. The product (FOM) was identified by thin layer chromatography and microbiological assay. Under the culture conditions of 28 degrees C pH7.5 and 280 r/min, 0.3% concentration of PPOH, reached a level of 2.2 mg/ml of FoM and the yield was 41%. When the PPOH concentration was 0.6%, the yield was 8%.
Shi
J
J
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Cui
F
F
Ge
M
M
D001711:Biotransformation; D003470:Culture Media; D005578:Fosfomycin / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010407:Penicillium / Q000378:metabolism*; D013213:Starch / Q000378:metabolism; D014508:Urea / Q000378:metabolism
D003470:Culture Media; D009943:Organophosphorus Compounds; C074765:propenylphosphonic acid; D005578:Fosfomycin; D014508:Urea; D013213:Starch