[Isolation of moniliformin-degrading bacterium Ochrobactrum sp. and analysis of its functional properties].
A moniliformin(MON)-degrading bacterium strain, named as Y21-2, was isolated from the mycotoxin-contaminated soil from Heilongjiang Province by the enrichment microculture technique. This strain can grow with MON as its sole carbon and energy source. In the minimal medium with 500 micrograms/mL MON, the number of cells increased from 10(7) to 10(10). Traditional taxonomy, assays of its G + C content and 16S rDNA sequence homology identified Y21-2 as Rhizobiaceae, Ochrobactrum sp. Resting cell suspensions prepared from induced Y21-2 can degrade MON with great speed, which also suggested the existence of enzymes committed to MON degradation in the cell.
Chen
W
W
College of Biological Sciences, China Agricultural University, Beijing 100094.
Zhang
H
H
Li
J
J
D001673:Biodegradation, Environmental; D003470:Culture Media; D003503:Cyclobutanes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D042041:Ochrobactrum / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D003470:Culture Media; D003503:Cyclobutanes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C008539:moniliformin