title: Epithelial cell surface sites involved in the polyvalent adherence of Porphyromonas gingivalis: a convincing role for neuraminic acid and glucuronic acid.
abstract: We investigated the target structures of the epithelial cells responsible for the attachment of Porphyromonas gingivalis by immunocytofluorimetry, enzyme-linked immunosorbent assay, and confocal microscopy. Integrins (beta1, beta3, and alphaV) and E-cadherin played no significant role. Carbohydrates (such as alpha-D-methylglucoside, L-fucose, D- and L-mannose, N-acetylglucosamine, and N-acetylgalactosamine) had little inhibitory effect on bacterial binding. Enzymatic treatments of the epithelial membranes and sugar competition studies showed that N-acetylneuraminic acid and glucuronic acid were involved in binding.
authors.affiliation: Equipe de Biologie Buccale, UPRES-EA 1256, 35000 Rennes, France.
authors.forename: G
authors.initials: G
authors.lastname: Agnani
authors.forename: S
authors.initials: S
authors.lastname: Tricot-Doleux
authors.forename: S
authors.initials: S
authors.lastname: Houalet
authors.forename: M
authors.initials: M
authors.lastname: Bonnaure-Mallet
mesh_terms: D001422:Bacterial Adhesion; D002462:Cell Membrane / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D020723:Glucuronic Acid / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007624:KB Cells / Q000382:microbiology; D018613:Microscopy, Confocal; D009438:Neuraminic Acids / Q000737:chemistry / Q000378:metabolism*; D016966:Porphyromonas gingivalis / Q000472:pathogenicity / Q000502:physiology*
chemical_list: D009438:Neuraminic Acids; D020723:Glucuronic Acid