title: [The clone and comparison of very virulent plus Marek's disease virus(vv+ DMV)648 strain glycoprotein E(gE) gene].
abstract: The glycoprotein E(gE) gene of very virulent plus Marek's Disease (vv+ MDV) 648 strain was amplified by polymerase chain reaction (PCR) and cloned into pUC18 vector. The gE sequence was determined and analysed. The whole length of 648 strain gE gene is 1494 base pairs. The protein encoded by gE gene has several features characteristic of a membrane-associated glycoprotein. It contains eight potential glycosylation sites, a markedly hydrophobic region at the N terminus that could function as a signal peptide and a hydrophobic segment (aa 391-419) at C terminus that could function as a transmembrane anchor element. Comparison of the gE between 648 strain and virulent MDV (vMDV) GA strain revealed that gE sequence is conserved in MDV serotype 1, there are only two basepairs different, (at bp 512 and 1472), which induce two amino acids changed respectively (at aa 171 and 491). The function of 648 strain gE and the difference of gE between 648 strain and GA strain is on study.
authors.affiliation: Animal and Veterinary Science College, Yangzhou University, Yangzhou 225009, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: Z
authors.initials: Z
authors.lastname: Cui
authors.forename: L
authors.initials: L
authors.lastname: He
authors.forename: S
authors.initials: S
authors.lastname: Zhu
authors.forename: A
authors.initials: A
authors.lastname: Qin
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D008381:Herpesvirus 2, Gallid / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000235:genetics*; D014774:Virulence
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; C106928:glycoprotein E, Marek's disease virus