title: [Production and some properties of a thermophilic protease from Bacillus stearothermophilus WF146].
abstract: The factors affecting Bacillus stearothermophilus WF146 for thermophilic protease producing have been investigated, more than 600 units of enzyme in 1 mL of fermented culture could be achieved under suitable condition. The protease had a molecular weight around 34 kD estimated by SDS-PAGE, and functioned optimally at pH 8.0 and 80 degrees C, respectively. In addition, the enzyme exhibited high temperature tolerance and was stable at a wide range of pH, and Ca2+ played a key role for the stability of the enzyme. While the protease activity of the enzyme was strongly inhibited by PMSF, DFP and IAA, and was not affected by DTT.
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan 430072.
authors.forename: B
authors.initials: B
authors.lastname: Tang
authors.forename: L
authors.initials: L
authors.lastname: Zhou
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Dai
authors.forename: Z
authors.initials: Z
authors.lastname: Peng
mesh_terms: D010450:Endopeptidases / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D004795:Enzyme Stability; D005285:Fermentation; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D013696:Temperature
chemical_list: D010450:Endopeptidases