Metastin-like immunoreactivity in the rat medulla oblongata and spinal cord.
Metastin, the product of metastasis suppressor gene KiSS-1, is proposed to be the natural ligand for the G-protein-coupled receptor GPR54, known also as AXOR12. This immunohistochemical study, using a rabbit polyclonal antiserum against the human metastin fragment (45-54)-NH(2), showed that in rats metastin-like immunoreactivity (MTS-LI) was present in neurons of the nucleus of the solitary tract and caudoventrolateral reticular nucleus, and in cell processes of the spinal trigeminal tract and lateral reticular nucleus. MTS-LI was confined mainly to neurons and fibers at or caudal to the area postrema. In the spinal cord, MTS-LI cell processes formed a dense plexus in superficial layers I and II of the dorsal horn. The pattern of distribution of MTS-LI in the medulla and spinal cord suggests that this novel peptide may participate in autonomic and sensory neural signaling.
Dun
Siok L
SL
Department of Pharmacology, James H. Quillen College of Medicine, East Tennessee State University, PO Box 70577, Johnson City, TN 37614, USA.
Brailoiu
G Cristina
GC
Parsons
Amy
A
Yang
Jun
J
Zeng
Qiang
Q
Chen
Xiangqun
X
Chang
Jaw Kang
JK
Dun
Nae J
NJ
D000818:Animals; D005260:Female; D006801:Humans; D007106:Immune Sera; D007150:Immunohistochemistry; D059648:Kisspeptins; D008297:Male; D008526:Medulla Oblongata / Q000737:chemistry*; D020671:Posterior Horn Cells / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D017552:Solitary Nucleus / Q000737:chemistry; D013116:Spinal Cord / Q000737:chemistry*; D025521:Tumor Suppressor Proteins
D007106:Immune Sera; C106628:KISS1 protein, human; C491406:Kiss1 protein, rat; D059648:Kisspeptins; D011506:Proteins; D025521:Tumor Suppressor Proteins