Toxoneuron nigriceps polydnavirus encodes a putative aspartyl protease highly expressed in parasitized host larvae.
Toxoneuron nigriceps (Viereck) (Hymenoptera: Braconidae) is an endophagous parasitoid of larval stages of the tobacco budworm, Heliothis virescens (F.) (Lepidoptera: Noctuidae). This parasitoid is associated with a polydnavirus (TnBV), injected at oviposition along with the egg, and involved in the disruption of host immune reaction and endocrine balance. This paper reports the molecular characterization of TnBV2, one of the most abundant genes in the TnBV genome. TnBV2 expression produces a mature 0.6 kb transcript in fat body, prothoracic glands and haemocytes, as early as 6 h after parasitoid oviposition. Only in haemocytes a specific longer transcript of 2.5 kb is found 24 h after parasitization. The putative translation product of TnBV2 contains a retroviral type aspartyl protease domain. The possible origin and functional role of this TnBV gene are discussed.
Falabella
Patrizia
P
Istituto di Genetica e Biofisica--C.N.R.--Via Pietro Castellino 111, 80131 Napoli, Italy.
Varricchio
Paola
P
Gigliotti
Silvia
S
Tranfaglia
Antonio
A
Pennacchio
Francesco
F
Malva
Carla
C
D000595:Amino Acid Sequence; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D015967:Gene Expression Regulation, Viral; D015723:Gene Library; D016679:Genome, Viral; D006927:Hymenoptera / Q000821:virology*; D017403:In Situ Hybridization; D007915:Lepidoptera / Q000469:parasitology*; D008969:Molecular Sequence Data; D018071:Polydnaviridae / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
D004279:DNA, Viral; D012367:RNA, Viral; D016282:Aspartic Acid Endopeptidases