Fast determination of propranolol in urine and pharmaceutical preparations by stopped-flow and micellar-stabilized room-temperature phosphorescence: validation of the method.
The stopped-flow mixing technique has been used to study the kinetic determination of propranolol by means of micellar-stabilized room-temperature phosphorescence. This mixing system diminishes the time required for the deoxygenation of micellar medium by sodium sulfite, allowing a kinetic curve that levels off within only 7s to be obtained. The phosphorescence enhancers thallium (I) nitrate, sodium dodecyl sulfate, and sodium sulfite were optimized to obtain maximum sensitivity and selectivity. A pH value of 6.54 was selected as adequate for phosphorescence development. The kinetic curves of propranolol phosphorescence were scanned at lambda(ex)=290 nm and lambda(em)=524 nm. The calibration graphs were linear for the concentration range from 25 to 400 ng mL(-1). The phosphorescence lifetime of propranolol is approximately 1210 micros. The detection limit calculated as proposed clayton was 13.53 ng mL(-1) and by applying the error propagation theory, the detection limit was 8.37 ng mL(-1). The repeatability was studied using 10 solutions of 200 ng mL(-1) of propranolol; if error propagation theory is assumed, the relative error is 1.94%. The standard deviation for a replicate sample was 4.0 ng mL(-1). This method was successfully applied to the determination of propranolol in commercial formulations and in urine. Suitable recovery values were obtained.
Murillo Pulgarín
J A
JA
Department of Analytical Chemistry and Foods Technology, University of Castilla-La Mancha, Campus Universitario Avda Camilo Jose Cela, 10, Ciudad Real 13071, Spain. joseantonio.murillo@uclm.es
Alañón Molina
A
A
Fernández López
P
P
Alañón Pardo
M T
MT
D006262:Health; D006801:Humans; D008163:Luminescent Measurements; D008823:Micelles; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011433:Propranolol / Q000032:analysis* / Q000652:urine*; D015203:Reproducibility of Results; D013696:Temperature; D013997:Time Factors
D008823:Micelles; D004364:Pharmaceutical Preparations; D011433:Propranolol