title: Anti-allergic action of buckwheat (Fagopyrum esculentum Moench) grain extract.
abstract: The anti-allergic action of buckwheat grain extract (BGE) was investigated using rodent experimental models. The oral, intraperitoneal and intradermal administration of BGE significantly inhibited the compound 48/80-induced vascular permeability documented by Evans blue extravasation. In addition, BGE showed potent inhibitory effect on passive cutaneous anaphylaxis (PCA) activated by anti-dinitrophenyl (DNP) IgE when orally administered. In an in vitro study, BGE revealed to possess inhibitory potential on the compound 48/80-induced histamine release from rat peritoneal mast cells (RPMC). Moreover, BGE inhibited the IL-4 and TNF-alpha mRNA induction by PMA and A23187 in human leukemia mast cells, HMC-1. Taken together, these results suggest that anti-allergic action of BGE may be due to the inhibition of histamine release and cytokine gene expression in the mast cells.
authors.affiliation: Oriental Hospital, Oriental Medical College of Daejeon University, 22-5 Daeheung-dong, Daejeon 301-724, South Korea.
authors.forename: Chang Deok
authors.initials: CD
authors.lastname: Kim
authors.forename: Won-Kyung
authors.initials: WK
authors.lastname: Lee
authors.forename: Kyong-Ok
authors.initials: KO
authors.lastname: No
authors.forename: Sang-Ki
authors.initials: SK
authors.lastname: Park
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Lee
authors.forename: Seok Rhin
authors.initials: SR
authors.lastname: Lim
authors.forename: Seok-Seon
authors.initials: SS
authors.lastname: Roh
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D019612:Fagopyrum / Q000737:chemistry*; D006632:Histamine / Q000378:metabolism; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D018926:Anti-Allergic Agents; D016207:Cytokines; D010936:Plant Extracts; D012333:RNA, Messenger; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine