[Study of the changes and effect of VEGF and bFGF in early stage of maxillofacial blast injury in rabbits].
OBJECTIVE
The aim of this study is to determine the content of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in the wound fluid during maxillofacial soft tissue blast injury, and to evaluate their effects on angiogenesis of wound healing.


METHODS
The rabbit model of maxillofacial blast injury was made by KTY-04 blasting cap. The content of VEGF and bFGF in wound fluid which was collected by using polying alcohol (PVA) sponge, were detected by ELISA. The distributions of VEGF and bFGF in the wound tissue were observed by using the techniques of in situ hybridization and immunohistochemistry.


RESULTS
In the wound fluid of maxillofacial blast injury, the content of VEGF was rising steadily at the first week after injury. Comparing with natural sera, it showed difference from the first day after injury, and showed obvious different from the third day (P < 0.01), on which the content was 2.9 ng/ml +/- 2.7 ng/ml. It reached the peak on the seventh day after injury. The bFGF level in wound fluid was 565 pg/ml +/- 436 pg/ml, the peak value was gained at the sixth hour after injury. In the following days, it declined close to the serum level three to five days after injury, and then moved up slightly on the seventh day after injury. The expression of VEGF was located in epithelial cells, vascular endothelial cells and some chronic inflammatory cells. The mRNA transcription of bFGF was present in fibroblasts, endothelial cells and macrophages.


CONCLUSIONS
VEGF and bFGF take part in two angiogenic cascades with synergistic action in maxillofacial blast injury. The bFGF stimulates the initial angiogenesis and induces the production of VEGF. The subsequent and more prolonged angiogenic stimuli can be mediated by VEGF.
Zhang
C
C
Department of Stomatology, Southwest Hospital, Third Military Medical University.
Li
H
H
Zhou
S
S
Yang
J
J
D000818:Animals; D001753:Blast Injuries / Q000378:metabolism*; D016228:Endothelial Growth Factors / Q000378:metabolism*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008222:Lymphokines / Q000378:metabolism*; D008297:Male; D008446:Maxillofacial Injuries / Q000378:metabolism*; D018919:Neovascularization, Physiologic; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014945:Wound Healing
D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D016222:Fibroblast Growth Factor 2