Visualization of molecular and cellular events with green fluorescent proteins in developing embryos: a review.
During the past 5 years, green fluorescent protein (GFP) has become one of the most widely used in vivo protein markers for studying a number of different molecular processes during development, such as promoter activation, gene expression, protein trafficking and cell lineage determination. GFP fluorescence allows observation of dynamic developmental processes in real time, in both transiently and stably transformed cells, as well as in live embryos. In this review, we include the most up-to-date use of GFP during embryonic development and point out the unique contribution of GFP visualization, which resulted in novel discoveries.
Yu
Yong A
YA
Division of Biochemistry, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA.
Szalay
Aladar A
AA
Wang
Gefu
G
Oberg
Kerby
K
D000818:Animals; D015415:Biomarkers; D019070:Cell Lineage; D004622:Embryo, Mammalian / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D047108:Embryonic Development; D005314:Embryonic and Fetal Development / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000032:analysis* / Q000235:genetics* / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics
D015415:Biomarkers; D008164:Luminescent Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins