title: Conserved requirement of Lim1 function for cell movements during gastrulation.
abstract: To investigate Lim1 function during gastrulation, we used transcript depletion through DEED antisense oligonucleotides in Xenopus and cell transplantation in mice. Xenopus embryos depleted of Lim1 lack anterior head structures and fail to form a proper axis as a result of a failure of gastrulation movements, even though mesodermal cell identities are specified. Similar disruption of cell movements in the mesoderm is also observed in Lim1(-/-) mice. Paraxial protocadherin (PAPC) expression is lost in the nascent mesoderm of Lim1(-/-) mouse embryos and in the organizer of Lim1-depleted Xenopus embryos; the latter can be rescued to a considerable extent by supplying PAPC exogenously. We conclude that a primary function of Lim1 in the early embryo is to enable proper cell movements during gastrulation.
authors.affiliation: Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Neil A
authors.initials: NA
authors.lastname: Hukriede
authors.forename: Tania E
authors.initials: TE
authors.lastname: Tsang
authors.forename: Raymond
authors.initials: R
authors.lastname: Habas
authors.forename: Poh-Lynn
authors.initials: PL
authors.lastname: Khoo
authors.forename: Kirsten
authors.initials: K
authors.lastname: Steiner
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Weeks
authors.forename: Patrick P L
authors.initials: PP
authors.lastname: Tam
authors.forename: Igor B
authors.initials: IB
authors.lastname: Dawid
mesh_terms: D000818:Animals; D005075:Biological Evolution; D019521:Body Patterning; D015820:Cadherins / Q000235:genetics; D002465:Cell Movement; D005775:Gastrula / Q000166:cytology* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization; D060850:LIM-Homeodomain Proteins; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D051379:Mice; D010641:Phenotype; D000091363:Protocadherins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors; D014981:Xenopus / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029867:Xenopus Proteins
chemical_list: D015820:Cadherins; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; C543964:Lhx1 protein, Xenopus; C499523:Lhx1 protein, mouse; C117635:Pcdh8 protein, Xenopus; D000091363:Protocadherins; D012333:RNA, Messenger; D014157:Transcription Factors; D029867:Xenopus Proteins