[Using L-form bacterium ATP bioluminescence assay for rapid testing L-form bacterial susceptibility].
Adenosine triphosphate is a kind of necessary metabolites in living cells. The authors detected ATP contents by using bioluminescence method in 111 strains of L-form bacteria after exposing to 5 kinds of antibiotics. The results showed that the mean value of CPM was less than (35 +/- 10.2)%.s-1. Thus, the value could be acted as a critical concentration between susceptibility and resistance. The conincidence rate of this method and K-B method was 95.3%. It indicates that the bioluminescence method has a high sensitivity. It can be used to detect L-form bacterium-drug susceptibility quickly and may play an important role for choosing the antibiotics.
Tang
Y
Y
Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha 410008.
Wen
B
B
Li
X
X
Yan
X J
XJ
Guo
S J
SJ
D000255:Adenosine Triphosphate / Q000032:analysis*; D002439:Cefotaxime / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology*; D006801:Humans; D007740:L Forms; D008163:Luminescent Measurements; D008826:Microbial Sensitivity Tests / Q000379:methods; D013211:Staphylococcus aureus / Q000187:drug effects*; D013291:Streptococcus / Q000187:drug effects
D002511:Cephalosporins; D000255:Adenosine Triphosphate; D002439:Cefotaxime