[Experimental research of protective and therapeutic effects of zinc and vitamin E on mouse liver radiational damage].
OBJECTIVE
To study the effects of zinc and vitamin E on lipid peroxide (LPO) contents and glutathione peroxidase (GSH-Px) activities in liver homogenates of radiational damage mice.


METHODS
Two hundred and forty mice were divided randomly into five groups: normal control group (Group A), 60Co gamma-ray irradiator group (Group B), 60Co + zinc (Zn) group (Group C), 60Co + vitamin E(VE) group (Group D), and 60Co + Zn + VE group (Group E). After irradiated with 60Co gamma-ray 7.5 Gy, the mice were protectively treated with Zn and VE at different times. LPO contents and GSH-Px activities in liver homogenates of the mice were determined.


RESULTS
The level of liver LPO was significantly higher (P < 0.01), but the level of liver GSH-Px was significantly lower (P < 0.01) in Group B than those in Group A; the content of liver GSH-Px was markedly higher in Group C and Group D than that in Group B (P < 0.01), while the content of liver LPO was markedly lower in Group D than that in Group B (P < 0.05); the concentration of liver GSH-Px was markedly higher in Group E than that in Group D (P < 0.01).


CONCLUSIONS
VE may play an important role in lowering liver LPO contents and raising liver GSH-Px activities in radiational damage mice. vitamin E can cooperate with zinc in raising liver GSH-Px activities, stopping liver cells from lipids peroxidation and keeping integrity of liver cell membrane.
Chen
J F
JF
Isotope Laboratory, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Shi
Q J
QJ
Zheng
S Y
SY
D000818:Animals; D003037:Cobalt Radioisotopes; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D051379:Mice; D011833:Radiation Injuries, Experimental / Q000378:metabolism / Q000517:prevention & control* / Q000628:therapy; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D011897:Random Allocation; D014810:Vitamin E / Q000494:pharmacology* / Q000627:therapeutic use; D015032:Zinc / Q000494:pharmacology* / Q000627:therapeutic use
D003037:Cobalt Radioisotopes; D008054:Lipid Peroxides; D011837:Radiation-Protective Agents; D014810:Vitamin E; D005979:Glutathione Peroxidase; D015032:Zinc