Irreversible binding of o,p'-DDD in interrenal cells of Atlantic cod (Gadus morhua).
Precision-cut tissue slices of the anterior kidney from Atlantic cod (Gadus morhua) were prepared with a Krumdieck tissue slicer and exposed to 2-(2-chlorophenyl)-2-(4-chloro-(14C)phenyl)-1,1-dichlorethane (o,p(')-[14C]DDD) in vitro. Microautoradiography revealed irreversible o,p(')-DDD-derived binding confined to the glucocorticoid producing interrenal cells (adrenocortical analogues). This cell-selective binding was confirmed by means of autoradiography at different levels of resolution on Atlantic cod administered o,p(')-[14C]DDD intragastrically. The results provide evidence for a site-specific metabolic activation and irreversible binding of o,p(')-DDD in the interrenal cells, which, in turn, may modify glucocorticoid homeostasis.
Lindhe
Orjan
O
Department of Environmental Toxicology, Uppsala University, Norbyvägen 18A, S-752 36 Uppsala, Sweden.
Brandt
Ingvar
I
Christiansen
Jørgen Schou
JS
Ingebrigtsen
Kristian
K
D000284:Administration, Oral; D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D001711:Biotransformation; D002250:Carbon Radioisotopes; D005399:Fishes; D066298:In Vitro Techniques; D007401:Interrenal Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008939:Mitotane / Q000378:metabolism* / Q000633:toxicity
D002250:Carbon Radioisotopes; D008939:Mitotane