Effect of acidity on the enantiomeric resolution of thyroxine and tocainide by HPLC on a (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid column.
Enantiomeric resolution of thyroxine and tocainide was achieved on a (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid column. The mobile phases were methanol/water (4:1, v/v) and methanol/water containing 5 mM sulfuric acid (4:1, v/v) for tocainide and thyroxine respectively. The flow rate was 0.5 ml/min. The effect of the acidity on the chiral resolution of these drugs was studied. Detection was at 220 nm for both drugs. The values of alpha and Rs were 2.08-3.11 and 1.00-2.60, respectively, for thyroxine while the values of alpha and Rs were 1.13-1.26 and 0.10-1.30, respectively, for tocainide.
Aboul-Enein
Hassan Y
HY
Biological and Medical Research Department (MBC-03), King Faisal Specialist Hospital and Research Center, PO Box 3354, Riyadh 11211, Saudi Arabia. enein@kfshrc.edu.sa
Ali
Imran
I
Hyun
Myung Ho
MH
Cho
Yoo Jae
YJ
Jin
Jong Sun
JS
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D043844:Crown Ethers; D004988:Ethers, Cyclic; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism; D013974:Thyroxine / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D016677:Tocainide / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification
D043844:Crown Ethers; D004988:Ethers, Cyclic; D012996:Solutions; D012997:Solvents; C090732:18-crown-6 2,3,11,12-tetracarboxylic acid; D016677:Tocainide; D013974:Thyroxine