[Determination of fatty acids in shark cartilage by GC-MS using microwave-assisted digestion and derivatization].
A rapid microwave-assisted digestion and derivatization method for the determination of fatty acids in shark cartilage by GC-MS was developed. The optimum conditions for digestion and derivatization were studied in detail using orthogonal design. The digestion and derivatization were accomplished in 4 minutes at 600 W microwave power using HCl-methanol (1:4, V/V) as digestion and derivatization solvent, and the extraction of the target analytes could be carried out simultaneously. This method is rapid, solvent-saving, and particularly suitable for the rapid determination of fatty acids in solid samples.
Li
G K
GK
Department of Chemistry, Zhongshan University, Guangzhou 510275, China.
He
X Q
XQ
Zhang
Z X
ZX
D000818:Animals; D002356:Cartilage / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008872:Microwaves; D012754:Sharks
D005227:Fatty Acids