[Effects of heat shock pretreatment on the translocation and expression of alpha B-crystallin in cultured neonatal rat cardiomyocytes].
The effects of heat shock pretreatment on translocation and expression of alpha B-crystallin were evaluated in neonatal rat cardiomyocytes.


RESULTS
1. Western blot analysis demonstrated that cytosolic soluble alpha B-crystallin rapidly translocated to insoluble intracellular structure at 10 or 30 minutes and returned to cytosolic soluble compartment at about 1-2 h after heat shock. 2. Double staining of immunofluorescence showed that alpha B-crystallin redistributed in cytosol after heat shock, while HSC70 translocated from cytosol to nucleus. 3. Quantitative analysis with immunoelectronic microscopy demonstrated alpha B-crystallin translocated to cytoskeletal structure such as Z-line and nuclear envelope after heat shock. 4. Northern-blot showed that heat shock induced alpha B-crystallin mRNA expression. The rapid translocation and increasing expression of alpha B-crystallin after heat shock suggests that this small heat shock protein may play an important protective roles during the early and late phases of myocardial ischemic preconditioning.
Xiao
W M
WM
Department of Pathophysiology, Xiangya Medical College, Central South University, Changsha 410078.
Yuan
K Y
KY
Ynan
C
C
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000494:pharmacology* / Q000502:physiology; D008297:Male; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D014178:Translocation, Genetic; D038203:alpha-Crystallin B Chain / Q000096:biosynthesis* / Q000235:genetics
D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D038203:alpha-Crystallin B Chain