title: [A study on pathological changes of closed diffuse brain injury in rats with H.E., esterification-silver stain and albumin immunohistochemical stain].
abstract: OBJECTIVE
  To observed the pathological changes of closed diffuse brain injury in the rats died immediately and 15 min to 5 days after the injury.
  
  
  METHODS
  H.E. staind and esterification-silver stain were applied to investigate the closed diffuse brain injury.
  
  
  RESULTS
  In rats died immediately after the concussive injury, a number of shrunken neurons(type I change), distended neurons(type II change) and wave-like nerve fibers were identified in the brain tissue, especially in brain-stem. At 2 h and 8 h after injury, brain edema and axonal swelling appeared clearly in the cortex and white matter, especially in brain-stem. At post-traumatic 8 h and 24 h, the axonal retraction balls began to appear. The amount of neurons undergoing type I and II changes and constraction balls increased along with the survivor time. After 4 days and 5 days, brain edema alleviated, but the retraction balls and axonal swelling still existed. With Esterifica-tion silver stain, the above changes of neurons and nerve fibers were more obvious. With ABC stain, the distribution of albumin(Al) was extended from the perio-vascular area to diffuse distribution. Al positive staining were more obvious in injuried neurons and nerve fibers.
  
  
  CONCLUSIONS
  The distribution of the concussive damage in the brain are coup, contra-coup and centripental.
authors.affiliation: Department of Forensic Medicine, Medical College of Beihua University, Jilin 132001.
authors.forename: X J
authors.initials: XJ
authors.lastname: Yu
authors.forename: F
authors.initials: F
authors.lastname: Xiao
authors.forename: J W
authors.initials: JW
authors.lastname: Wu
authors.forename: M Y
authors.initials: MY
authors.lastname: Wu
mesh_terms: D000418:Albumins; D000818:Animals; D001696:Biomechanical Phenomena; D001921:Brain / Q000473:pathology*; D016489:Head Injuries, Closed / Q000473:pathology*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D016622:Silver Staining
chemical_list: D000418:Albumins