title: Optical characterization of armadillo acyl-CoA binding protein.
abstract: Acyl-CoA binding protein (ACBP) and fatty acid binding protein (FABP) are intracellular transporters of activated and free fatty acids, respectively. Unlike other tissues with active lipid metabolism, armadillo Harderian gland contains much more ACBP than FABP. To characterize armadillo ACBP structure and binding properties, we produced it in Escherichia coli and carried out detailed fluorescence and circular dichroism spectroscopy studies. The K(D) for palmitoyl-CoA, measured directly by fluorescence and rotatory power, was 34+/-12 and 75+/-39 nM, respectively. The structure of armadillo ACBP appears to be very similar to that of bovine and rat liver ACBPs.
authors.affiliation: Facultad de Farmacia y Bioquímica (UBA-CONICET), Instituto de Química y Fisicoquímica Biológicas, Juni;n 956, 1113, Buenos Aires, Argentina.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Cavagnari
authors.forename: Daniel
authors.initials: D
authors.lastname: Milikowski
authors.forename: Jorge F
authors.initials: JF
authors.lastname: Haller
authors.forename: María C
authors.initials: MC
authors.lastname: Zanek
authors.forename: José A
authors.initials: JA
authors.lastname: Santomé
authors.forename: Mario R
authors.initials: MR
authors.lastname: Ermácora
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001135:Armadillos; D001483:Base Sequence; D002417:Cattle; D002942:Circular Dichroism; D026261:Diazepam Binding Inhibitor / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry; D051381:Rats; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013816:Thermodynamics; D014466:Ultraviolet Rays
chemical_list: D026261:Diazepam Binding Inhibitor; D011506:Proteins