[Preparation and evaluation of amide-octyl-bonded phase for the separation of basic substances in reversed-phase liquid chromatography].
A new bonded-phase, amide-octyl-bonded phase (AOBP), with an internal polar functional group-amide was firstly prepared in domestic by two-step reaction process. The amino phase was formed in the first step and further modified in the second step by attaching an alkyl chain via reaction of an acid chloride with the amino ligand. Characterization of prepared packing was carried out with elemental analysis and Fourier transform infrared (FTIR) spectrometry. Hydrophobicity, selectivity and silanophilic activity of AOBP were evaluated by using organic components including acidic, bascic and neutral analytes and methanol-water as binary mobile phase. The applicable ranges of pH and stability of AOBP were also evaluated. The results showed that AOBP has excellent chromatographic properties and resistance to hydrolysis between pH = 2.5-7.5, It can be used for the separation of basic solutes efficiently.
Huang
X J
XJ
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, China.
Liu
Y
Y
Cong
R Z
RZ
Liu
X L
XL
Wang
J D
JD
D020003:1-Octanol / Q000737:chemistry; D000577:Amides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D009281:Naphthalenes / Q000032:analysis; D009578:Nitrobenzenes / Q000032:analysis; D011437:Propylamines; D012821:Silanes; D017550:Spectroscopy, Fourier Transform Infrared; D014498:Uracil / Q000032:analysis*
D000577:Amides; D009281:Naphthalenes; D009578:Nitrobenzenes; D011437:Propylamines; D012821:Silanes; C088294:3-aminopropyltrimethoxysilane; C031721:naphthalene; D014498:Uracil; C036077:nitrobenzene; D020003:1-Octanol