[Study on sampling preconcentration method combining electrostacking with isotachophoresis in capillary electrophoresis].
A sampling preconcentration method combining electrostacking with isotachophoresis is presented in this paper. The optimum conditions for electrostacking and isotachophoresis were investigated. With the preconcentration conditions of 70 s electrostacking and 40 s isotachophoresis, two medicines, propranolol and metoprolol, with similar structures were separated by capillary zone electrophoresis. The working voltage for all the steps was 15 kV. Being adjusted to pH 4.0, 30 mmol/L NaAc-HAc, 30 mmol/L beta-alanine-HAc and 1.5 mmol/L NaAc-HAc were employed as the background, viz. leading, terminating and sample buffer solutions respectively. In comparing with the conventional electro-migration injection, the enhancement factors in peak height of the present method were about 250 and 160 for propranolol and metoprolol respectively. Total analysis time was similar to that of the conventional one.
Li
M
M
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
He
Y Z
YZ
Gan
W E
WE
Lin
X Q
XQ
Yang
L
L
Qu
Q S
QS
D004573:Electrolytes; D004586:Electrophoresis / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D008790:Metoprolol / Q000302:isolation & purification*; D011433:Propranolol / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods
D004573:Electrolytes; D011433:Propranolol; D008790:Metoprolol