[Thermodynamic characteristics of stoichiometric displacement linear parameter log I in reversed-phase liquid chromatography].
Based on the physical meaning of each term in the linear parameter log I (a constant correlating the affinity of solute to stationary phase) of stoichiometric displacement model for retention(SDM-R) of solute, the thermodynamic characteristics of log I of solute in reversed-phase liquid chromatography (RPLC) were investigated theoretically. This point was tested by experimental data with two linear relationships: (1) plot of log I vs 1/T (T is absolute temperature) with apolar, polar small solutes and proteins; (2) plot of log I vs log Po/w (partition coefficient of solute between n-octanol and water) with apolar and polar small solutes. From the comparison of the magnitude of partition coefficient between small solute and proteins, a phenomenon that the resolution of small solutes depends on column length but that of proteins almost to be independent of column length is quantitatively explained.
Bai
Q
Q
Institute of Modern Separation Science, Key Laboratory of Modern Separation Science in Shaanxi Province, Northwest University, Xi'an 710069, China. baiquan@nwu.edu.cn
Geng
X D
XD
D002853:Chromatography, Liquid / Q000379:methods*; D003574:Cytochrome c Group / Q000032:analysis; D007328:Insulin / Q000032:analysis*; D016014:Linear Models; D008333:Mandelic Acids / Q000032:analysis*; D010795:Phthalic Acids / Q000032:analysis; D013816:Thermodynamics
D003574:Cytochrome c Group; D007328:Insulin; D008333:Mandelic Acids; D010795:Phthalic Acids; C032279:phthalic acid; C037938:mandelic acid