title: 3-0 methylglucose absorption in vivo correlates with nutrient absorption and intestinal surface area in experimental short bowel syndrome.
abstract: BACKGROUND
  Inert carbohydrate probes are widely used to study intestinal permeability and examine the passive uptake of markers. This study examined the use of quantifying 3-0 methylglucose (3-0 MG) absorption as a marker of intestinal surface area and active nutrient transport capability.
  
  
  METHODS
  Using a rat model, varying degrees of short bowel syndrome (SBS) were induced: sham operation (intestinal transection only), 50% resection (R50), 75% resection (R75), and 90% resection (R90; n = 6 to 8 in each group). Animals were pair fed, and over days 5 and 6 postoperation, a balance study was done to quantify absorption of dietary fat, protein, and carbohydrate. On day 7, animals were gavaged with the test solution containing 3-0 MG, lactulose, and mannitol, followed by a 16-hour urine collection. Urine recovery of probes was quantified using high-performance liquid chromotography. Animals were then killed, and the gross and microscopic intestinal morphology was determined.
  
  
  RESULTS
  As expected, increased resection resulted in reduced absorption of dietary nutrients and 3-0 MG, which reached significance in the R90 resected animals. 3-0 MG absorption was significantly correlated with intestinal surface area and the absorption of dietary protein and fat (p < .01 for all comparisons). Interestingly, 3-0 MG absorption was not significantly correlated with the absorption of dietary carbohydrate.
  
  
  CONCLUSIONS
  3-0 MG absorption is a useful marker of functional intestinal surface area and of the absorption of dietary fat and protein in experimental SBS. 3-0 MG may be a useful marker of nutrient absorptive capacity in patients with SBS; further study is indicated.
authors.affiliation: Gastrointestinal Research Group, University of Calgary, AB, Canada.
authors.forename: Gary R
authors.initials: GR
authors.lastname: Martin
authors.forename: Jonathan B
authors.initials: JB
authors.lastname: Meddings
authors.forename: David L
authors.initials: DL
authors.lastname: Sigalet
mesh_terms: D019325:3-O-Methylglucose / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000033:anatomy & histology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012778:Short Bowel Syndrome / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D019325:3-O-Methylglucose