title: Emergence in Klebsiella pneumoniae of a chromosome-encoded SHV beta-lactamase that compromises the efficacy of imipenem.
abstract: A Klebsiella pneumoniae isolate was identified that had reduced susceptibility to several expanded-spectrum cephalosporins and imipenem. That isolate produced a chromosome-encoded SHV-type beta-lactamase, SHV-38, that had an alanine to valine substitution in position Ambler 146 compared to beta-lactamase SHV-1. The kinetic parameters for purified beta-lactamases SHV-38 and SHV-1 showed that the hydrolytic spectrum of SHV-38 included only ceftazidime and imipenem. This report is the first example of an SHV-type beta-lactamase capable of hydrolyzing imipenem.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Le Kremlin-Bicêtre, Paris, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.forename: Claire
authors.initials: C
authors.lastname: Héritier
authors.forename: Isabelle
authors.initials: I
authors.lastname: Podglajen
authors.forename: Wladimir
authors.initials: W
authors.lastname: Sougakoff
authors.forename: Laurent
authors.initials: L
authors.lastname: Gutmann
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D000900:Anti-Bacterial Agents / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006801:Humans; D015378:Imipenem / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged; D001618:beta-Lactamases / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D015378:Imipenem; C054882:beta-lactamase PIT-2; D001618:beta-Lactamases