[Construction of plasmid gene bank of V. cholerae O139 and detection of O-antigen genes].
Because O-antigen biosynthesis genes are a tandem gene cluster. Gnomic fragments of 4-20 kilobases (kb) were obtained by digesting genomic DNA of V. cholerae O139 with restriction endonuclease EcoRI, then plasmid gene bank was constructed. Recombinant colony, E. coli DH5 alpha (pMG320), expressing O-antigen of V. cholerae O139 was detected from the bank by immunological agglutinative reaction. The futher analysis showed O-antigen expressed by recombinant colony had both immunogenicity and reactogenicity, and the size of O-antigen biosynthesis genes was about 4.6 kb.
Qu
D
D
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100850, China.
Liu
C
C
Zhang
Y
Y
Ma
Q
Q
D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D015698:Genomic Library; D019081:O Antigens / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D010957:Plasmids; D014734:Vibrio cholerae / Q000235:genetics* / Q000276:immunology
D004269:DNA, Bacterial; D019081:O Antigens