Detection of multiple splice variants of the nuclear protein phosphatase 1 regulator sds22 in rat liver nuclei.
Antipeptide antibodies generated against the N terminus of the protein phosphatase 1 (PP1) binding protein sds22 detected at least four forms of the protein in a rat liver nuclear extract. Four of these immunoreactive bands likely correspond to four predicted forms of sds22 that are generated by alternative splicing. These four proteins are expressed at different levels and appear to be localized exclusively in the nucleus, and two of these proteins copurify with PPI on the protein phosphatase affinity matrix microcystin-Sepharose. Two higher molecular mass nuclear proteins that are immunoreactive with the sds22 antibodies also copurify on microcystin-Sepharose and may be novel forms of sds22 expressed in mammalian cells.
Tran
Hue T
HT
Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada.
Bridges
Dave
D
Ulke
Annegret
A
Moorhead
Greg B G
GB
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002467:Cell Nucleus / Q000737:chemistry* / Q000201:enzymology; D008099:Liver / Q000737:chemistry* / Q000201:enzymology; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins; D010749:Phosphoprotein Phosphatases / Q000737:chemistry* / Q000302:isolation & purification; D054645:Protein Phosphatase 1; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C509753:Ppp1r7 protein, rat; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1