title: A Drosophila full-length cDNA resource.
abstract: BACKGROUND
  A collection of sequenced full-length cDNAs is an important resource both for functional genomics studies and for the determination of the intron-exon structure of genes. Providing this resource to the Drosophila melanogaster research community has been a long-term goal of the Berkeley Drosophila Genome Project. We have previously described the Drosophila Gene Collection (DGC), a set of putative full-length cDNAs that was produced by generating and analyzing over 250,000 expressed sequence tags (ESTs) derived from a variety of tissues and developmental stages.
  
  
  RESULTS
  We have generated high-quality full-insert sequence for 8,921 clones in the DGC. We compared the sequence of these clones to the annotated Release 3 genomic sequence, and identified more than 5,300 cDNAs that contain a complete and accurate protein-coding sequence. This corresponds to at least one splice form for 40% of the predicted D. melanogaster genes. We also identified potential new cases of RNA editing.
  
  
  CONCLUSIONS
  We show that comparison of cDNA sequences to a high-quality annotated genomic sequence is an effective approach to identifying and eliminating defective clones from a cDNA collection and ensure its utility for experimentation. Clones were eliminated either because they carry single nucleotide discrepancies, which most probably result from reverse transcriptase errors, or because they are truncated and contain only part of the protein-coding sequence.
authors.affiliation: Berkeley Drosophila Genome Project Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. staple@fruitfly.org
authors.forename: Mark
authors.initials: M
authors.lastname: Stapleton
authors.forename: Joe
authors.initials: J
authors.lastname: Carlson
authors.forename: Peter
authors.initials: P
authors.lastname: Brokstein
authors.forename: Charles
authors.initials: C
authors.lastname: Yu
authors.forename: Mark
authors.initials: M
authors.lastname: Champe
authors.forename: Reed
authors.initials: R
authors.lastname: George
authors.forename: Hannibal
authors.initials: H
authors.lastname: Guarin
authors.forename: Brent
authors.initials: B
authors.lastname: Kronmiller
authors.forename: Joanne
authors.initials: J
authors.lastname: Pacleb
authors.forename: Soo
authors.initials: S
authors.lastname: Park
authors.forename: Ken
authors.initials: K
authors.lastname: Wan
authors.forename: Gerald M
authors.initials: GM
authors.lastname: Rubin
authors.forename: Susan E
authors.initials: SE
authors.lastname: Celniker
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D001483:Base Sequence / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis*; D030541:Databases, Genetic; D004331:Drosophila melanogaster / Q000737:chemistry / Q000235:genetics*; D017344:Genes, Insect; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D018076:DNA, Complementary