Rapid detection of fungal filaments in corneal scrapings by microwave heating-assisted Grocott's methenamine silver staining.
The Gomoris methanamine silver impregnation technique is a highly reliable and archiveable method of detecting fungal filaments, but the staining procedure is time consuming and laborious. A technique using microwave energy to reduce the duration of Gomori's silver staining is described.
Vemuganti
Geeta K
GK
Ophthalmic Pathology Service, L V Prasad Eye Institute, L V Prasad Marg, Banjara Hills, Hyderabad-500 034, India. geeta@lvpeye.stph.net
Naidu
Chenchu
C
Gopinathan
Usha
U
D003315:Cornea / Q000382:microbiology* / Q000473:pathology; D015821:Eye Infections, Fungal / Q000175:diagnosis / Q000382:microbiology*; D005658:Fungi / Q000302:isolation & purification; D006361:Heating / Q000379:methods; D006801:Humans; D007634:Keratitis / Q000175:diagnosis / Q000382:microbiology*; D008872:Microwaves; D009181:Mycoses / Q000175:diagnosis / Q000382:microbiology*; D012680:Sensitivity and Specificity; D016622:Silver Staining / Q000295:instrumentation / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods