[Study on susceptibility test of pathogenic fungi from otorhinolaryngology by Etest method].
OBJECTIVE
To study the sensitivity of pathogenic fungi from otorhinolaryngology to 5 antifungal agents of Ketoconazole (KE), Itraconazole(IT), Fluorocytosine(FC), Amphtericin B (AP) and Fluconazole(FL) by Etest method.


METHODS
Etest for determine MIC of 14 pathogenic fungi was performed according to the manufacturer's instructions. The Etest strip containing the 5 antifungal drugs were putted on medium with Candida albicans, A. flarus and A. fumigatus etc., respectively, to determine MIC of antifungal drugs.


RESULTS
Candida albicans were sensitized to all the above 5 antifungal drugs, KE and IT were most sensitized. Seven fungi such as A. flavus, A fumigatus, A. oryzae etc were all sensitized to KE and IT. The MIC range of KE and IT against 15 strains of pathogenic fungi were < or = 0.008-2 mg/L and < or = 0.006-4 mg/L respectively. In 15 strains, 11 strains showed drug-resistant to FL, 8 strains to AP and FC, 4 strains to KE and 1 strains to IT respectively and MIC were all > 32 mg/L.


CONCLUSIONS
Etest MIC were in good agreement with macrobroth MIC. The use of Etest strips for antifungal susceptibility test is a new and promising method with advantage of easy to perform, exact results and satisfactory reproducibility. Etest is valuable on clinical practice.
Li
J
J
Department of Dermatology, First Affiliated Hospital, Guangxi Medical University, Nanning 530021.
Nong
H
H
Nong
D
D
Cheng
L
L
D000935:Antifungal Agents / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009181:Mycoses / Q000382:microbiology*; D010038:Otorhinolaryngologic Diseases / Q000382:microbiology*; D015203:Reproducibility of Results
D000935:Antifungal Agents