B cell responses in gastric antrum and duodenum following oral inactivated Helicobacter pylori whole cell (HWC) vaccine and LT(R192G) in H pylori seronegative individuals.
To investigate whether B cell-specific responses could be elicited in the gastric mucosa of Helicobacter pylori (HP) naive subjects, five volunteers ingested three doses of a HP killed whole cell (HWC) vaccine with 25 microg of recombinant heat-labile toxin (LT(R192G)). Two of three subjects had detectable LT(R192G) and HWC IgA antibody secreting cell (ASC) gastric responses. LT(R192G) and HWC responses in duodenal were 5-14-fold higher than those detected in antral biopsies (P<0.01 and P=0.05, respectively). These results provide the first evidence that specific gastric B cell responses can be induced in HP-non-infected individuals following oral immunization.
Losonsky
Genevieve A
GA
Department of Pediatrics, Baltimore, MD 21201, USA. losonskyg@medimmune.com
Kotloff
Karen L
KL
Walker
Richard I
RI
D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D004386:Duodenum / Q000166:cytology* / Q000276:immunology; D004768:Enterotoxins / Q000276:immunology*; D029968:Escherichia coli Proteins; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D006868:Hydrolysis; D018928:Immunity, Mucosal / Q000276:immunology; D007114:Immunization; D007070:Immunoglobulin A / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D011706:Pyloric Antrum; D013270:Stomach / Q000166:cytology* / Q000276:immunology; D014357:Trypsin; D014614:Vaccines, Synthetic / Q000276:immunology*
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D014614:Vaccines, Synthetic; C027538:heat-labile enterotoxin, E coli; D014357:Trypsin