[Preparation of immobilized metal-chelated affinity membrane and its application to purification of Cu/Zn-superoxide dismutase].
Immobilized metal-chelated affinity membranes were prepared using macropore cellulose filter paper as matrix. The matrix was treated with alkaline, activated with epichlorohydrin and coupled with iminodiacetate sodium, and then Cu2+ was immobilized. Cu/Zn-superoxide dismutase(SOD) was purified with prepared affinity membranes. The special activity of the purified Cu/Zn-SOD was increased 10.7 times from 645 U/mL to 6,882 U/mL. The protein recovery and the activity recovery were 92.3% and 985%, respectively. A new method to prevent the leaking of metal ion was developed and Cu2+ concentration in the effluent was lowered to 86 micrograms/L.
Wei
Q
Q
Department of Bioengineering, South China University of Technology, Guangzhou 510641, China.
Yao
R H
RH
Bao
S X
SX
D002614:Chelating Agents; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods; D013482:Superoxide Dismutase / Q000302:isolation & purification*
D002614:Chelating Agents; D013482:Superoxide Dismutase