title: [Research of toxin and plasmids of Saccharomyces cerevisiae].
abstract: Killer toxin from Saccharomyces cerevisiae SK was isolated by ultrafiltration of culture supernatants and purified by poly(ethylene glycol). The toxin migrates as one single protein band on SDS-PAGE and its molecular weight is 15 kD. The SK toxin has the greatest lethal effect on the sensitive yeast strain in the lat-lag phase. Extraction and purification of killer heretity factor(dsRNA) from SK found that M-dsRNA plasmid and L-dsRNA plasmid have different molecular lengths being 1.7 kb and 4.0 kb.
authors.affiliation: Department of Microbiology, Shandong University, Jinan 250100.
authors.forename: Y
authors.initials: Y
authors.lastname: Qin
authors.forename: D
authors.initials: D
authors.lastname: Gao
mesh_terms: D055646:Killer Factors, Yeast; D008970:Molecular Weight; D009183:Mycotoxins / Q000737:chemistry / Q000302:isolation & purification*; D010957:Plasmids; D012330:RNA, Double-Stranded / Q000235:genetics; D012331:RNA, Fungal / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D055646:Killer Factors, Yeast; D009183:Mycotoxins; D012330:RNA, Double-Stranded; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins