[Partial gene clone and nif gene homologous sequence analysis of Streptococcus sanguis].
OBJECTIVE
To analyze the sequence of Streptococcus sanguis chromosome which contains one DNA fragment of 800 base pairs (bp) and discuss Streptococcus sanguis biological features of heredity.


METHODS
Streptococcus DNA of 800-bp genetic fragment was cloned and analyzed by using eukaryotic expression vector.


RESULTS
By Genbank database, it showed that the 800-bp genetic sequence was highly homologous with other bacterial nifS and nifU gene, and the highest homologous score was 114.


CONCLUSIONS
This nif gene of ATCC 10556 strain may correlate with nutrient metabolism and peroxide hydrogen release of Streptococcus sanguis.
Pan
Y
Y
Department of Oral Medicine, Dental School, China Medical University.
Wang
H
H
Jin
C
C
Sun
K
K
D003001:Cloning, Molecular; D005798:Genes, Bacterial; D009586:Nitrogen Fixation / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013298:Streptococcus sanguis / Q000235:genetics*