[Extract of cemental matrix enhance periodontal cells bind to the root surface].
OBJECTIVE
To investigate whether the extract of cemental matrix can enhance periodontal cells binding to the root surfaces.


METHODS
Healthy human gingiva and periodontal ligament were acquired from patients, then gingival fibroblasts and periodontal cells were cultured in vitro. On the other hand, extract of cemental matrix was separated and clarified from healthy teeth which were extracted because of orthodontic treatment. Finally, the effects of cementum matrix with different concentration and different time on the attachment of gingival fibroblast and periodontal cells to root surface were observed.


RESULTS
The extract of cemental matrix could enhance the initial attachment of human gingival fibroblasts (HGF) and human periodontal cells (HPC) to the surface of root, and such effects were strengthened with increasing density and time. The optimal concentration of extract of cell attachment 10 micrograms/ml, and the attachment was also proportional to the incubation time, reaching near maximal levels at 2 hours, furthermore, the extract of cemental matrix was more effective in promoting the attachment of human periodontal cells than that of gingival fibroblasts.


CONCLUSIONS
The extract of cemental matrix can enhance the initial attachment of HGF and HPC on the root surface.
Sui
W
W
College of Stomatology, Fourth Military Medical University.
Hong
Y
Y
Xiao
M
M
D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000494:pharmacology*; D002478:Cells, Cultured; D003739:Dental Cementum / Q000737:chemistry*; D005347:Fibroblasts / Q000502:physiology*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology*; D013997:Time Factors; D014092:Tooth Root
D015815:Cell Adhesion Molecules