title: Effects of implantation site of dead stem cells in rats with stroke damage.
abstract: Searching for valid control grafts, we assessed the performance of rats subjected to middle cerebral artery occlusion (MCAO) and grafted with freeze-thawed dead stem cells into sites previously used for active grafts (ipsilateral and contralateral striatum and ventricle) on bilateral asymmetry and water maze tests. We expected to find that sham grafted groups had impairments equivalent to those of MCAO-only controls, relative to intact controls. This proved to be the case for contralateral and intraventricular grafts, and for asymmetry in rats with ipsilateral grafts. However, spatial learning was substantially impaired and lesion volume was increased by 55% with ipsilateral dead cell grafts. Exacerbation of stroke effects indicates potential hazards in the use of dead cells for sham grafts.
authors.affiliation: Institute of Psychiatry, Neuroimaging Research Group-Neurology P042, De Crespigny Park, London SE5 8AF, UK.
authors.forename: Michel
authors.initials: M
authors.lastname: Modo
authors.forename: R Paul
authors.initials: RP
authors.lastname: Stroemer
authors.forename: Ellen
authors.initials: E
authors.lastname: Tang
authors.forename: Sara
authors.initials: S
authors.lastname: Patel
authors.forename: Helen
authors.initials: H
authors.lastname: Hodges
mesh_terms: D000818:Animals; D016923:Cell Death; D002460:Cell Line / Q000637:transplantation; D002552:Cerebral Ventricles; D003342:Corpus Striatum; D004292:Dominance, Cerebral; D005615:Freezing; D020244:Infarction, Middle Cerebral Artery / Q000601:surgery*; D007249:Inflammation; D007859:Learning Disabilities / Q000209:etiology*; D008297:Male; D018782:Maze Learning; D011597:Psychomotor Performance; D051381:Rats; D017207:Rats, Sprague-Dawley; D012107:Research Design; D033581:Stem Cell Transplantation / Q000009:adverse effects*; D016042:Transplantation, Heterotopic