Serotonin receptor mRNA expression in rat dorsal root ganglion neurons.
In the present study, we have used in situ hybridization to examine the distribution of serotonin (5-HT) receptors in rat dorsal root ganglion (DRG) neurons. Within DRG neurons, mRNAs for 5-HT1B, 5-HT1D, 5-HT2A, 5-HT2B, 5-HT3B and 5-HT4 receptors were readily detected in small (<25 microm), medium (25-45 microm) and large (>45 microm) diameter neurons. In contrast mRNAs for 5-HT1A, 5-HT1E, 5-HT2C, 5-HT5A, 5-HT5B, 5-HT6 and 5-HT7 receptors were undetectable in these neurons. The present study provides an insight into the molecular profile of 5-HT receptor subtypes in neurons responsible for modulating sensory information.
Nicholson
R
R
Pfizer Global Research and Development, Cambridge University Forvie Site, Robinson Way, CB2 2QB, Cambridge, UK.
Small
J
J
Dixon
A K
AK
Spanswick
D
D
Lee
K
K
D000818:Animals; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D017403:In Situ Hybridization; D008297:Male; D009474:Neurons / Q000145:classification / Q000378:metabolism*; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011985:Receptors, Serotonin / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011985:Receptors, Serotonin