Cutaneous hypersensitivity reactions to Psoroptes ovis and Der p 1 in sheep previously infested with P. ovis--the sheep scab mite.
We have previously shown that infestation with Psoroptes ovis induces an IgE response and intense tissue eosinophilia, typical of a Type I hypersensitivity response [Parasite Immunol. 22 (2000) 407]. Intradermal tests (IDSTs) suggest that there are also delayed and Arthus-type responses to this parasite. In order to study the nature of ovine cutaneous reactions to P. ovis, naïve controls and experimentally infested sheep (n = 5) were challenged intradermally with mite antigen. Challenge elicited immediate (P < 0.001) and delayed (P < 0.005) wheal reactions in sensitised sheep. At 6 (P < 0.02) and 30 h (P < 0.001) the predominant infiltrating cells were eosinophils. To explore the role of circulating antibodies, naïve sheep (n = 5) were subjected to Prausnitz-Kustner (PK) tests. These elicited immediate (P < 0.02) but not delayed wheal reactions. At 6 h eosinophils (P < 0.001) dominated the infiltrate. These results suggest that P. ovis allergens provoke an IgE-dependent immediate and late phase response and a cell-mediated eosinophil-rich delayed-type hypersensitivity response (ER-DTH).
van den Broek
A H M
AH
Department of Veterinary Clinical Studies, Veterinary Field Station, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Veterinary Centre, Easter Bush, Roslin Midlothian EH25 9RG, UK. adri.vandenbroek@ed.ac.uk
Huntley
J F
JF
Halliwell
R E W
RE
Machell
J
J
Taylor
M
M
Miller
H R P
HR
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D060829:Arthropod Proteins; D003546:Cysteine Endopeptidases; D006098:Granulocytes / Q000166:cytology / Q000276:immunology; D006967:Hypersensitivity / Q000276:immunology* / Q000662:veterinary*; D007958:Leukocyte Count; D008407:Mast Cells / Q000166:cytology / Q000276:immunology; D008924:Mite Infestations / Q000276:immunology / Q000662:veterinary; D040042:Psoroptidae / Q000276:immunology*; D012757:Sheep Diseases / Q000276:immunology / Q000469:parasitology; D034561:Sheep, Domestic / Q000276:immunology* / Q000469:parasitology*; D012867:Skin / Q000276:immunology*; D013997:Time Factors
D000906:Antibodies; D000941:Antigens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D003546:Cysteine Endopeptidases; C451162:Dermatophagoides pteronyssinus antigen p 1