[Preliminary study on citral impaires the Aspergillus flavus membrane].
Compared with the normally growing A. flauas, the content as below was determined: the utilization ratio to protein and reducing sugar of hyphostroma poisened by citral, the activity of [Na+, K+]-ATPase capable of decomposition ATP, and the seepagevity ratio of electrolyte. In addition, the shape change in spore was observed via the scanning electron microscope (SEM) and the fast multi-channel micro-spectrophotomer (FMCM). The result above all suggested facts as following after it's poised by the citral in MIC. The surface of hyphostroma and spore turned into be porous and rough. The pass trace on spore shriveled and closed. The rate of conduct electricity increased by 52.8%. The utilization ratio to protein and reducing sugar respectively decreased 61.5% and 44.3%. The rate of spore's sprout dropped to 61.4%. The molecular structure of membrane was so distinctly changed that it lost the selective permeability. There was inhibition on hyphostroma growth and spore sprout.
Luo
M
M
Department of Biological Engineering, Anhui Agricultural University, Hefei 230036, China.
Jiang
L
L
Wu
Z
Z
D000042:Absorption; D000080462:Acyclic Monoterpenes; D000255:Adenosine Triphosphate / Q000378:metabolism; D001231:Aspergillus flavus / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D002241:Carbohydrates / Q000493:pharmacokinetics; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D039821:Monoterpenes / Q000494:pharmacology*; D010539:Permeability; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013172:Spores, Fungal / Q000254:growth & development / Q000648:ultrastructure
D000080462:Acyclic Monoterpenes; D002241:Carbohydrates; D039821:Monoterpenes; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase; C007076:citral