[Determination of three active ingredients in food additive sucrose fatty acid ester by TLC scanning].
A method of separation and quantitation of monoester, diester and triester in food additive sucrose fatty acid esters by TLC with dual-wavelength TLC scanner in the sawtooth scanning mode is described. The detective wavelength was 530 nm and the reference wavelength was 700 nm. A mixture of chloroform, methanol, acetic acid and water (80:10:8:1, V/V) was used as mobile phase. The calibration curve was linear over a range from 4 micrograms to 60 micrograms with correlation coefficients of 0.9949-0.9980. The average recoveries were 96.45%-98.73% (n = 3, RSD = 2.7%-3.2%). This method is accurate, simple and dependable, with a wide range of linearity. It has been applied to the analysis of various samples and can be used for the quality control of the food additive sucrose fatty acid ester.
Xie
X T
XT
Department of Chemistry, Yunnan Normal University, Kunming 650092, China.
Zhang
H P
HP
Zheng
P
P
Luo
C Y
CY
D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis; D005503:Food Additives / Q000032:analysis*; D013395:Sucrose
D005227:Fatty Acids; D005503:Food Additives; D013395:Sucrose