title: Cartilage regeneration using slow release of bone morphogenetic protein-2 from a gelatin sponge to treat experimental canine tracheomalacia: a preliminary report.
abstract: We investigated whether saber sheath-type tracheomalacia could be treated by the slow release of bone morphogenetic protein (BMP)-2 from a gelatin sponge. A 1 cm gap was made in the middle portion of each of 10 consecutive tracheal cartilage rings in the canine cervix (control group, n = 3), then a gelatin sponge containing 12 microg of BMP-2 solution was implanted in the gap (12 microg group, n = 3). In another group (120 microg + P group, n = 3), the implanted gelatin sponge contained 120 microg of BMP-2 solution, and the gap was covered with periosteum. All of the control dogs developed saber sheath-type tracheomalacia, whereas tracheomalacia was not observed in the 12 microg and 120 microg + P groups. In the 12 microg group, fibrous cartilage was observed at the ends of the cartilage stumps. In the 120 microg + P group, newly formed bone and cartilage were observed to form a bridge between the cartilage stumps. The regeneration of cartilage or bone induced by the slow release of BMP-2 from a gelatin sponge might be useful for treatment of tracheomalacia.
authors.affiliation: Second Department of Surgery, Kagawa Medical University, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa, Japan 761-0793.
authors.forename: Taku
authors.initials: T
authors.lastname: Okamoto
authors.forename: Yasumichi
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Masashi
authors.initials: M
authors.lastname: Gotoh
authors.forename: Dage
authors.initials: D
authors.lastname: Liu
authors.forename: Minoru
authors.initials: M
authors.lastname: Kihara
authors.forename: Kotaro
authors.initials: K
authors.lastname: Kameyama
authors.forename: Eiichi
authors.initials: E
authors.lastname: Hayashi
authors.forename: Kenji
authors.initials: K
authors.lastname: Nakamura
authors.forename: Akira
authors.initials: A
authors.lastname: Yamauchi
authors.forename: Cheng-Long
authors.initials: CL
authors.lastname: Huang
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Yokomise
authors.forename: Masaya
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D001999:Bronchoscopy; D002356:Cartilage / Q000502:physiology*; D002357:Cartilage Diseases / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D004285:Dogs; D005780:Gelatin; D012038:Regeneration / Q000187:drug effects*; D015503:Surgical Sponges; D014133:Tracheal Diseases / Q000188:drug therapy* / Q000473:pathology; D016212:Transforming Growth Factor beta
chemical_list: D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D016212:Transforming Growth Factor beta; D005780:Gelatin