[The synthesis of phenyl-silica gel and its application in purification of taxol].
Phenyl-silica gels were synthesized with four methods of reaction on gamma-aminopropyltriethoxysilane (APTS) silica gel. The synthesized gels were used as stationary phase of normal pressure chromatography to separate and purify taxol. The comparison of these four silica gels was carried out. It was found that the gel synthesized by gas-phase reaction gave the best results. The purity of taxol was 77.4%, and the recovery was about 90%.
Tian
G L
GL
State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080, China. tg313@263.net
Zhang
Z Q
ZQ
Su
Z G
ZG
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D017239:Paclitaxel / Q000302:isolation & purification*; D011437:Propylamines; D012821:Silanes; D058428:Silica Gel; D012822:Silicon Dioxide
D011437:Propylamines; D012821:Silanes; D058428:Silica Gel; D012822:Silicon Dioxide; C477625:amino-propyl-triethoxysilane; D017239:Paclitaxel