title: Evaluation of adjuvants for protein vaccines against tuberculosis in guinea pigs.
abstract: Subunit vaccines against tuberculosis show promise but require administration with adjuvants to stimulate relevant immune responses for protection. Guinea pigs are the model of choice for evaluating protective immunity to aerogenic challenge with virulent mycobacteria, but few studies have been undertaken to identify suitable adjuvants for vaccine screening in this species. Here, we compare the efficacy of several adjuvants to induce T cell responses to culture filtrate protein in guinea pigs. We report that of several adjuvants tested, the most promising was CpG ODN formulated in an aqueous emulsion. This adjuvant induced type 1 T cell responses equivalent to that of FIA, as measured by delayed-type hypersensitivity reactions (DTH), antigen-specific T cell proliferation and antigen-specific IgG1 and IgG2 responses. These data demonstrate the potential for CpG motif based adjuvants for use in TB vaccine screening in guinea pigs, and other diseases where a type 1 T cell response is required.
authors.affiliation: Department of Bacterial Diseases, Veterinary Laboratories Agency Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK. p.j.hogarth@vla.defra.gsi.gov.uk
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Hogarth
authors.forename: Keith J
authors.initials: KJ
authors.lastname: Jahans
authors.forename: Rolf
authors.initials: R
authors.lastname: Hecker
authors.forename: R Glyn
authors.initials: RG
authors.lastname: Hewinson
authors.forename: Mark A
authors.initials: MA
authors.lastname: Chambers
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D004353:Drug Evaluation, Preclinical; D005260:Female; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D007074:Immunoglobulin G / Q000097:blood; D008213:Lymphocyte Activation; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage; D013601:T-Lymphocytes / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology* / Q000517:prevention & control*; D032581:Tuberculosis Vaccines / Q000008:administration & dosage*; D022223:Vaccines, Subunit / Q000008:administration & dosage
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; C408982:CPG-oligonucleotide; D007074:Immunoglobulin G; D009838:Oligodeoxyribonucleotides; D032581:Tuberculosis Vaccines; D022223:Vaccines, Subunit