title: Canine mammary tumor cells transfected with B7-1 or B7-2 stimulate proliferation of peripheral blood mononuclear cells.
abstract: Neoplastic cells are believed to evade the immune system due, in part, to their inability to successfully provide a secondary, costimulatory signal for a T lymphocyte proliferative response. This report describes the generation and investigation of genetically engineered canine mammary tumor (CMT) cells that express canine B7-1, canine B7-2, or human B7-2. These transfected cells were used as stimulators in an allogeneic, costimulation assay. CMT cells transfected with canine B7-1 induced the greatest proliferation (7-fold increase), followed by CMT cells transfected with canine B7-2 (5-fold increase). The specificity of the canine B7-2 stimulatory response was demonstrated by a 38% reduction in proliferation caused by an anti-canine B7-2 blocking antibody. These results suggest that canine mammary tumor cells transfected with canine B7-1 or canine B7-2 may be useful for immunotherapeutic purposes.
authors.affiliation: Department of Pathobiology, 166 Greene Hall, Auburn University, AL 36849, USA.
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Whitley
authors.forename: Karen S
authors.initials: KS
authors.lastname: Sellins
authors.forename: Sandra J
authors.initials: SJ
authors.lastname: Ewald
authors.forename: Bruce F
authors.initials: BF
authors.lastname: Smith
authors.forename: Shumin
authors.initials: S
authors.lastname: Yang
authors.forename: Eva A
authors.initials: EA
authors.lastname: Sartin
authors.forename: Lauren G
authors.initials: LG
authors.lastname: Wolfe
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D018122:B7-1 Antigen / Q000235:genetics / Q000276:immunology*; D051940:B7-2 Antigen; D002999:Clone Cells; D004285:Dogs; D005260:Female; D006801:Humans; D016233:Immunotherapy, Active / Q000379:methods; D007371:Interferon-gamma / Q000276:immunology / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D007371:Interferon-gamma