Timing of nuclear maturation of nonstored and stored domestic cat oocytes.
In this study we compared the effects of preculture storage of ovaries, IVM medium, a reduced O(2) atmosphere and duration of culture on in vitro maturation (IVM) of domestic cat oocytes. One randomly selected ovary of each pair (69 pairs) was stored in PBS at 10 degrees C for 16-24h before oocyte recovery. The second ovary from each pair was used as a nonstored control. In Experiment I, we investigated the effect of culture media (TCM 199 versus SOF) and a reduced O(2) atmosphere (a humidified gas atmosphere of either 5% CO(2) in air or 5% CO(2):5% O(2):90% N(2)) on IVM of both stored and nonstored oocytes. In the second experiment, we compared timing of nuclear maturation of both stored and nonstored oocytes cultured for 17-18, 20-21, 24-26, 28-30, 33-34 or 42-45 h before being evaluated for meiotic status. In both, Experiments I and II, the recovery rate, quality and competence for maturation of oocytes originating from stored ovaries did not differ (P>0.05) compared with nonstored. In Experiment I, neither culture medium (37.5 versus 43.2% of Metaphase II, respectively in TCM 199 versus SOF) or gas atmosphere (40.0 versus 32.5% of Metaphase II, respectively in 5% CO(2) in air versus 5% CO(2):5% O(2):90% N(2)) affected oocyte maturation. In Experiment II, the mean proportion of oocytes achieving Metaphase II within 17-18 h of culture was 36.1% and did not significantly increase (P>0.05) over time up to 28 h. The highest proportion of oocytes (67.3%) reached Metaphase II stage after 42-45 h of culture. Therefore, we conclude that two "waves" of nuclear maturation of cat oocytes can be distinguished. The first wave takes place within 26 h and it is likely that most oocytes of this wave mature by 17-18 h; the second wave occurs after 28-30 h of IVM. It can be assumed that this double wave may reflect the presence of two oocyte populations with two different degrees of "prematuration" which require different lengths of IVM.
Katska-Ksiazkiewicz
Lucyna
L
PienGen Biomedical Corporation, 7417 Kingston Pike, Knoxville, TN 37919, USA. l.katska@izoo.krakow.pl
Ryńska
Bozenna
B
Kania
Gabriela
G
Smorag
Zdzisław
Z
Gajda
Barbara
B
Pieńkowski
Marek
M
D000818:Animals; D002415:Cats / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology*; D003470:Culture Media; D005260:Female; D008677:Metaphase / Q000502:physiology; D009865:Oocytes / Q000254:growth & development* / Q000502:physiology; D009866:Oogenesis / Q000502:physiology*; D010053:Ovary / Q000166:cytology; D010100:Oxygen; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary*; D013997:Time Factors
D003470:Culture Media; D010100:Oxygen