[The effect of N+ implantation on SOD activity of Deinococcus radiodurans and induction of Mn-SOD].
The induction of Mn-SOD and effect of N+ of 20 keV implantation on SOD activity of D. radiodurans were investigated. The results were as follows: (1) SOD activity of D. radiodurans had a little change when implantation dose was less than 8 x 10(14) N+/cm2, and increased gradually from 8 x 10(14) to 60 x 10(14) N+/cm2, and then decreased rapidly; (2) the test of adding H2O2 and CHCl3-CH3CH2OH which could inhibit different types of SOD showed that most SOD activity of D. radiodurans was constituted by Fe-SOD in general physiology and less then 8 x 10(14)N+/cm2 dose, and higher implantation dose induced increase of Mn-SOD activity.
Song
D
D
Centre of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031.
Li
H
H
Wang
J
J
Yao
J
J
Yu
Z
Z
D034301:Deinococcus / Q000187:drug effects / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D009584:Nitrogen / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism*
D004791:Enzyme Inhibitors; D013482:Superoxide Dismutase; D009584:Nitrogen