title: Expression of human pendrin in diseased thyroids.
abstract: We examined pendrin expression in various diseased thyroid tissues by immunohistochemistry (IHC) using antiserum raised against human pendrin and by real-time quantitative RT-PCR. In normal thyroids the antiserum reacted with the apical membrane of follicular cells and its immunoreactivity was faint. In Graves' thyroids, the IHC expression of pendrin appeared in a pattern similar to that of normal thyroids but it was more extensive and stronger, especially in areas showing marked proliferation of follicular cells. The immunoreactivities of pendrin in nodular goiters varied from case to case. In follicular adenomas, pendrin was localized in the follicle-forming parts of the tumor but was negative in trabecular parts. Pendrin was negative in all follicular carcinomas, papillary carcinomas, and in one case of medullary carcinoma. In quantitive mRNA analysis, the relative values of pendrin mRNA were significantly low in papillary carcinoma (p<0.01), whereas the values in other diseased thyroids were not significantly different from those in normal thyroids. These results suggest that pendrin may play a role in thyroid hormone production as the apical porter of chloride/iodide and investigation of pendrin leads to a better understanding of functional aspects of the iodine transportation system in thyroid diseases.
authors.affiliation: Department of Pathology, Yamanashi Medical University School of Medicine, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kondo
authors.forename: Nobuki
authors.initials: N
authors.lastname: Nakamura
authors.forename: Koichi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Murata
authors.forename: Akira
authors.initials: A
authors.lastname: Muramatsu
authors.forename: Akira
authors.initials: A
authors.lastname: Kawaoi
authors.forename: Ryohei
authors.initials: R
authors.lastname: Katoh
mesh_terms: D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D007150:Immunohistochemistry; D026901:Membrane Transport Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000076988:Sulfate Transporters; D013959:Thyroid Diseases / Q000378:metabolism*; D013961:Thyroid Gland / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D007106:Immune Sera; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; C085609:SLC26A4 protein, human; D000076988:Sulfate Transporters