[The preparation of norA gene probe in Staphylococcus aureus].
OBJECTIVE
To prepare norA gene probe for studies on the mechanism of resistance to fluoroquinolones mediated by norA gene in S. aureus.


METHODS
The Dig-labeled norA gene probe was made by PCR.


RESULTS
This method is simple and feasible. We have got a lot of probes in a short time. The sensitivity of norA gene probe is high; the probe is secure, easy to use and can be stored for a long time.


CONCLUSIONS
The probe is applicable to further studies on the mechanism of resistance to fluoroquinolones mediated by norA in S. aureus.
Zhong
L
L
Department of Infectious Diseases, First Affiliated Hospital, WCUMS, Chengdu 610041, China.
Feng
P
P
Fan
X
X
Lü
X
X
Lei
B
B
D000890:Anti-Infective Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D015342:DNA Probes / Q000096:biosynthesis*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D024841:Fluoroquinolones; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D016133:Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000187:drug effects
D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D015342:DNA Probes; D024841:Fluoroquinolones; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C075040:NorA protein, Staphylococcus