title: [Electron spin resonance studies on the oxidative denaturation of hemoglobin of blood preserved at 20 degrees C].
abstract: In order to further understand the injurious change of erythrocytes during blood preservation at room temperature, we studied the denaturation of oxidative hemoglobin of blood preserved at 20 degrees C using electron spin resonance (ESR) technique. The results showed: when blood was preserved at 20 degrees C, there was ESR absorption of low spin hemichrome (g = 2) during the 204-hour preservation period; when the blood was preserved at 4 degrees C after it had been preserved at 20 degrees C for 24 hours, there were ESR absorption of the high spin methemoglobin (g = 6) and ESR absorption of low spin hemichrome (g = 2) during the 40-day preservation period. This study demonstrates that ESR absorption will increase when preservation time increases. When blood is preserved at 4 degrees C, no ESR absorption will emerge during 40 days' preservation.
authors.affiliation: Division of Physics, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
authors.forename: C
authors.initials: C
authors.lastname: Feng
authors.forename: X
authors.initials: X
authors.lastname: Hu
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Li
mesh_terms: D000328:Adult; D001793:Blood Preservation; D004578:Electron Spin Resonance Spectroscopy; D006454:Hemoglobins; D006801:Humans; D008297:Male; D008706:Methemoglobin / Q000032:analysis*; D010084:Oxidation-Reduction; D011489:Protein Denaturation; D013696:Temperature; D013997:Time Factors
chemical_list: D006454:Hemoglobins; D008706:Methemoglobin