title: [The determination sorbitol and carbohydrates in nane fruit by high performance liquid chromatography].
abstract: A high performance liquid chromatographic method for the determination of carbohydrates and sorbitol in Nane fruit is described. The chromatography was carried out on a mu-Spherogel carbohydrate column(4.6 mm i.d. x 300 mm) (at 85 degrees C) using water as mobile phase and refractive index detector. The average recovery of carbohydrates were 75.00%-88.37% and correlation coefficients were 0.9709-0.9999. The relative standard deviations for intra-day were 5.10% for sucrose, 3.15% for glucose, 3.03% for fructose and 4.84% for sorbitol respectively (n = 5). The accumulations of soluble carbohydrates(including fructose, glucose, sorbitol and sucrose) in flesh showed their characteristic patterns in different special phase of fruit growth.
authors.affiliation: Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China.
authors.forename: G Y
authors.initials: GY
authors.lastname: Tang
authors.forename: S X
authors.initials: SX
authors.lastname: Guo
authors.forename: H J
authors.initials: HJ
authors.lastname: Wu
authors.forename: D M
authors.initials: DM
authors.lastname: Pan
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005632:Fructose / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D027861:Prunus / Q000737:chemistry*; D013012:Sorbitol / Q000032:analysis*; D013395:Sucrose / Q000032:analysis
chemical_list: D005632:Fructose; D013012:Sorbitol; D013395:Sucrose