Generation of an adenoviral vector containing an addition of a heterologous ligand to the serotype 3 fiber knob.
As an initial assessment of the feasibility of employing the adenovirus serotype 3 (Ad3) fiber knob as a locale for introducing a tropism-modifying motif, we generated an adenoviral vector containing a six-histidine tag genetically fused to the carboxy-terminus of the Ad3 fiber knob. The heterologous tag proved to be accessible for binding in the context of the virion and, moreover, had rendered the modified vector capable of mediating gene transfer through an artificial, non-Ad3 receptor.
Uil
Taco G
TG
Department of Medicine, Division of Human Gene Therapy, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
Seki
Toshiro
T
Dmitriev
Igor
I
Kashentseva
Elena
E
Douglas
Joanne T
JT
Rots
Marianne G
MG
Middeldorp
Jaap M
JM
Curiel
David T
DT
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001483:Base Sequence; D002213:Capsid / Q000502:physiology*; D003587:Cytomegalovirus / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins; D006639:Histidine / Q000235:genetics; D008024:Ligands; D008156:Luciferases / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic
D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D008024:Ligands; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D006639:Histidine; D008156:Luciferases