[Asymmetric microbial reduction of organosilyl ketone with immobilized Saccharomyces cerevisiae cells].
Asymmetric microbial reduction of acetyltrimethylsilane with immobilized Saccharomyces cerevisiae cells was studied. The effects of shake speeds, substrate concentration, immobilized cell concentration, pH and reaction temperature on the initial reaction rate, maximum yield and optical purity of the product were systematically studied. All above mentioned parameters have important effects on the raction. The optimum shake speed, substrate concentration, immobilized cell concentration, pH and reaction temperature were found to be 150 r/min, 14 mmol/L, 0.15 g/mL, 7.3 and 25 degrees C-30 degrees C respectively for the reaction, under which the maximum yield and optical purity of the product were as high as 84.9% and 90.2% ee.
Lou
Wenyong
W
Department of Biotechnology, South China University of Technology, Guangzhou 510640, China.
Zong
Minhua
M
Fan
Xiaodan
X
Lu
Jiequan
J
Du
Wei
W
D018914:Cells, Immobilized; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature; D014297:Trimethylsilyl Compounds / Q000378:metabolism*
D014297:Trimethylsilyl Compounds; C477044:acetyltrimethylsilane