title: Interaction of histone acetylases and deacetylases in vivo.
abstract: Having opposing enzymatic activities, histone acetylases (HATs) and deacetylases affect chromatin and regulate transcription. The activities of the two enzymes are thought to be balanced in the cell by an unknown mechanism that may involve their direct interaction. Using fluorescence resonance energy transfer analysis, we demonstrated that the acetylase PCAF and histone deacetylase 1 (HDAC1) are in close spatial proximity in living cells, compatible with their physical interaction. In agreement, coimmunoprecipitation assays demonstrated that endogenous HDACs are associated with PCAF and another acetylase, GCN5, in HeLa cells. We found by glycerol gradient sedimentation analysis that HATs are integrated into a large multiprotein HDAC complex that is distinct from the previously described HDAC complexes containing mSin3A, Mi-2/NRD, or CoREST. This HDAC-HAT association is partly accounted for by a direct protein-protein interaction observed in vitro. The HDAC-HAT complex may play a role in establishing a dynamic equilibrium of the two enzymes in vivo.
authors.affiliation: Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamagoe
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Kanno
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kanno
authors.forename: Shigakazu
authors.initials: S
authors.lastname: Sasaki
authors.forename: Richard M
authors.initials: RM
authors.lastname: Siegel
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Lenardo
authors.forename: Glen
authors.initials: G
authors.lastname: Humphrey
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Wang
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nakatani
authors.forename: Bruce H
authors.initials: BH
authors.lastname: Howard
authors.forename: Keiko
authors.initials: K
authors.lastname: Ozato
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry / Q000378:metabolism*; D018797:Cell Cycle Proteins; D002454:Cell Differentiation; D031541:Fluorescence Resonance Energy Transfer; D006367:HeLa Cells; D051548:Histone Acetyltransferases; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D046912:Multiprotein Complexes; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors; D020298:U937 Cells; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor
chemical_list: D000123:Acetyltransferases; D018797:Cell Cycle Proteins; D051548:Histone Acetyltransferases; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases; D046911:Macromolecular Substances; D046912:Multiprotein Complexes; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C501179:KAT2A protein, human; C110550:HDAC1 protein, human