[Cloning and sequencing analysis of rice stripe virus genome segment 4 of Chinese isolate Y].
The cDNA fragment covering full-length sequence of RStV RNA4 of Yunnan isolate in China was obtained by RT-PCR. The PCR-derived fragment was then cloned into vector pCRII. The cloned cDNA was sequenced. Comparison of the nucleotide and deduced amino acid sequences with those of the Japanese isolate T was made. The results showed that at the nucleotides level, vORF, vcORF and the intergenic region had 94.9%, 94.1% and 86.1% identity respectively, the 5'-untranslational region was exactly the same as that of Japanese isolate T, while the 3'-terminal sequence had 96.1% identity, differing by two nucleotides; at the amino acid level, vORF and vcORF had 99.4% and 98.3% identity respectively. Therefore, as well as being exactly the same size for the two isolates, the amino acid sequences of the coding regions and the 5'- and 3'-terminal sequences were well conserved. Our results indicated that the Chinese isolate is closely related to the Japanese isolate T.
Qu
Z
Z
Institute of Genetics, Fudan University, Shanghai 200433.
Shen
D
D
Deng
K
K
Lu
Y
Y
Li
C
C
Hull
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D016679:Genome, Viral; D007564:Japan; D008969:Molecular Sequence Data; D012275:Oryza / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D029364:Tenuivirus / Q000235:genetics* / Q000302:isolation & purification
D012367:RNA, Viral