title: [Study of pathogenetic role of Epstein-Barr virus in Hungarian patients with B-cell non-Hodgkin lymphomas].
abstract: BACKGROUND
  The Epstein-Barr virus is a member of herpesvirus family. It plays an important role in the etiopathogenesis of Burkitt lymphoma, other B-cell non-Hodgkin lymphomas, nasopharyngeal carcinoma, X chromosome-linked lymphoproliferative disease, Hodgkin lymphomas and a part of T-cell lymphomas. It has been suggested that association of Epstein-Barr virus with lymphomas and its pathological significance in disease varies in different geographical areas. The aim of our study was to reveal the role of Epstein-Barr virus in B-cell non-Hodgkin lymphomas diagnosed in Hungary. The authors asked: A) What is the frequency of the presence of virus genome in the biopsy specimen from B-cell non-Hodgkin patients? B) Which types of virus latency can be observed?
  
  
  METHODS
  Genomic DNA and EBER-specific RNA of Epstein-Barr virus in paraffin-embedded specimens were detected by polymerase chain reaction and in situ hybridisation, respectively.
  
  
  RESULTS
  Out of 36 B-cell non-Hodgkin lymphoma cases, 16 (45%) were found to be positive for Epstein-Barr virus by the above methods. Expression of latency genes, nuclear antigene-2 and latent membrane protein-1 was studied by immunohistochemical technique. The 16 virus-positive non-Hodgkin lymphoma cases showed Epstein-Barr virus latency types II. (37%) or III. (63%).
  
  
  CONCLUSIONS
  The authors data suggest that Epstein-Barr virus may be associated with the development of B-cell non-Hodgkin lymphomas in Hungarian patients.
authors.affiliation: Debreceni Egyetem, Orvos és Egészségtudományi Centrum, Altalános Orvostudományi Kar, Gyermekklinika.
authors.forename: Péter
authors.initials: P
authors.lastname: Germán
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Beck
authors.forename: Imre
authors.initials: I
authors.lastname: Semsei
authors.forename: Sándor
authors.initials: S
authors.lastname: Kiss
authors.forename: Balázs
authors.initials: B
authors.lastname: Görög
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Balogh
authors.forename: Ferenc
authors.initials: F
authors.lastname: Tóth
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Nemes
authors.forename: László
authors.initials: L
authors.lastname: Pajor
authors.forename: Eva
authors.initials: E
authors.lastname: Oláh
mesh_terms: D001483:Base Sequence; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D006814:Hungary; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D016393:Lymphoma, B-Cell / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000302:isolation & purification
chemical_list: D012367:RNA, Viral