title: Phosphorylation of serine residues is fundamental for the calcium-binding ability of Orchestin, a soluble matrix protein from crustacean calcium storage structures.
abstract: Orchestia cavimana is a terrestrial crustacean, which cyclically stores calcium in diverticula of the midgut, in the form of calcified amorphous concretions. These concretions are associated with a proteinaceous matrix, the main constituent of the soluble matrix is Orchestin, an acidic calcium-binding protein [Testenière et al., Biochem. J. 361 (2002) 327-335]. In the present paper, we clearly demonstrate that Orchestin is phosphorylated on serine and tyrosine residues, but that calcium binding only occurs via the phosphoserine residues. To our knowledge, this is the first example of an invertebrate mineralization for which a post-translational modification is clearly related to an important function of a calcifying protein.
authors.affiliation: UMR CNRS 5548, Développement-Communication chimique, Université de Bourgogne, 6 Bd Gabriel, F-21000 Dijon, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Hecker
authors.forename: Olivier
authors.initials: O
authors.lastname: Testenière
authors.forename: Frédéric
authors.initials: F
authors.lastname: Marin
authors.forename: Gilles
authors.initials: G
authors.lastname: Luquet
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002113:Calcification, Physiologic / Q000502:physiology; D002118:Calcium / Q000737:chemistry* / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D003445:Crustacea; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011485:Protein Binding / Q000502:physiology; D011499:Protein Processing, Post-Translational; D012694:Serine / Q000737:chemistry; D013329:Structure-Activity Relationship; D014443:Tyrosine / Q000737:chemistry
chemical_list: D002135:Calcium-Binding Proteins; C446561:orchestin protein, Orchestia cavimana; D014443:Tyrosine; D012694:Serine; D002118:Calcium