Effect of lead exposure on the erythrocytic antioxidant levels in goats.
Fifteen adult female goats were orally exposed to 5.46 mg lead (as lead acetate) per kg body weight daily for 2 weeks to study the antioxidant enzymes of the erythrocyte, lipid peroxide level, total thiol groups and total antioxidant status (TAS) in plasma. Ten goats served as unexposed control. Blood samples were collected before exposure (day 0) and on days 7 and 14. Ten per cent erythrocyte haemolysate was prepared and analysed for glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), total thiol groups and lipid peroxide. TAS was determined in plasma. There was a significant (P < 0.05) increase of erythrocytic GPx, SOD, CAT, total thiol groups and TAS on day 7 which was followed by a significant (P < 0.05) decrease of all these parameters on day 14. Lipid peroxide level increased significantly (P < 0.05) and the maximum level was attained by day 14. The results obtained indicate a possible role of free radicals in lead poisoning pathogenicity.
Mousa
H M
HM
Department of Veterinary Medicine, College of Agriculture and Veterinary, King Saud University, Al-Qaseem, Saudi Arabia. hasmousa@hotmail.com
Al-Qarawi
A A
AA
Ali
B H
BH
Abdel Rahman
H A
HA
ElMougy
S A
SA
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000097:blood / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood / Q000187:drug effects; D015511:Goat Diseases / Q000097:blood / Q000378:metabolism*; D006041:Goats; D007854:Lead / Q000008:administration & dosage / Q000633:toxicity*; D007855:Lead Poisoning / Q000378:metabolism / Q000662:veterinary*; D008054:Lipid Peroxides / Q000097:blood / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000097:blood / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood / Q000187:drug effects
D000975:Antioxidants; D008054:Lipid Peroxides; D013438:Sulfhydryl Compounds; D007854:Lead; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase