Does antigen-specific cytokine response correlate with the experience of oculorespiratory syndrome after influenza vaccine?
During the 2000-2001 season in Canada, a newly identified oculorespiratory syndrome (ORS) was observed in patients after immunization with inactivated influenza vaccine. ORS was associated with a high proportion of microaggregates of unsplit virions in the implicated vaccine and had clinical features suggesting delayed-onset hypersensitivity. We explore the association between in vitro cytokine balance (type 1 vs. type 2) and clinical ORS after influenza vaccination. We report the balance of interferon (IFN)-gamma, interleukin (IL)-10, IL-5, and IL-13 expression by peripheral blood mononuclear cells (PBMC) among unvaccinated, vaccinated ORS-affected, and vaccinated ORS-unaffected persons after in vitro challenge with implicated and nonimplicated vaccines. Antigen-stimulated PBMC from vaccinated persons produced significantly more IFN-gamma than did those from unvaccinated persons. There was a statistically significant type 2 polarization among unvaccinated compared with vaccinated persons. Although vaccinated ORS-affected individuals had less of a type 1 basis than did vaccinated unaffected individuals, this difference was not statistically significant.
Skowronski
Danuta M
DM
University of British Columbia Centre for Disease Control, Epidemiology Services, Vancouver, British Columbia, Canada. danuta.skowronski@bccdc.ca
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Hang
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Warrington
Richard
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Hegele
Richard G
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De Serres
Gaston
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HayGlass
Kent
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Stark
Donald
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White
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Macnabb
Jane
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Yan
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Manson
Heather E
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Brunham
Robert C
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D000328:Adult; D000956:Antigens, Viral / Q000276:immunology*; D002170:Canada; D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D005128:Eye Diseases / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007252:Influenza Vaccines / Q000009:adverse effects* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009975:Orthomyxoviridae / Q000276:immunology*; D012140:Respiratory Tract Diseases / Q000276:immunology*; D012307:Risk Factors; D013577:Syndrome; D015164:Vaccines, Inactivated / Q000009:adverse effects / Q000276:immunology
D000956:Antigens, Viral; D016207:Cytokines; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated