Erythropoietin prevents ischemia-reperfusion from inducing oxidative damage in fetal rat brain.
OBJECTIVE
The aim of this study was to show the effect of erythropoietin on ischemia-reperfusion-induced oxidative damage in fetal rat brain.


METHODS
Fetal brain ischemia was induced by clamping the utero-ovarian artery bilaterally for 20 min, and reperfusion was achieved by removing the clamps for 30 min. The control group was made up of non-injured rats that were 19 days pregnant. In the ischemia-reperfusion group no treatment was given, while 0.4 ml of human serum albumin solution and 5,000 U/kg recombinant human erythropoietin (r-Hu-EPO) were administered in the vehicle and treatment groups 30 min before ischemia-reperfusion injury. Lipid peroxidation in the brain tissue was determined as the concentration of thiobarbituric acid-reactive substances (TBARS) for each fetal rat. A one-way analysis of variance and the post-hoc test were used for statistical analysis.


RESULTS
TBARS increased to statistically significantly higher levels in fetal rat brain after ischemia-reperfusion injury than were found in the control group. Recombinant human erythropoietin prevented the increase in TBARS after ischemia-reperfusion injury.


CONCLUSIONS
Recombinant human erythropoietin has been shown to have neuroprotective effect in intrauterine ischemia-reperfusion-induced fetal brain damage in rats.
Solaroglu
Ihsan
I
Department of Neurosurgery, Ankara Numune Research and Educational Hospital, Ankara, Turkey.
Solaroglu
Ayse
A
Kaptanoglu
Erkan
E
Dede
Suat
S
Haberal
Ali
A
Beskonakli
Etem
E
Kilinc
Kamer
K
D000818:Animals; D001921:Brain / Q000098:blood supply / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism*; D004921:Erythropoietin / Q000494:pharmacology*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D010084:Oxidation-Reduction; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*
D011994:Recombinant Proteins; D004921:Erythropoietin