title: A new virus infecting Myzus persicae has a genome organization similar to the species of the genus Densovirus.
abstract: The genomic sequence of a new icosahedral DNA virus infecting Myzus persicae has been determined. Analysis of 5499 nt of the viral genome revealed five open reading frames (ORFs) evenly distributed in the 5' half of both DNA strands. Three ORFs (ORF1-3) share the same strand, while two other ORFs (ORF4 and ORF5) are detected in the complementary sequence. The overall genomic organization is similar to that of species from the genus DENSOVIRUS: ORFs 1-3 most likely encode the non-structural proteins, since their putative products contain conserved replication motifs, NTP-binding domains and helicase domains similar to those found in the NS-1 protein of parvoviruses. The deduced amino acid sequences from ORFs 4 and 5 show sequence similarities with the structural proteins of the members of the genus DENSOVIRUS: These data indicate that this virus is a new species of the genus Densovirus in the family PARVOVIRIDAE: The virus was tentatively named Myzus persicae densovirus.
authors.affiliation: Plant Research International, PO Box 16, 6700 AA Wageningen, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: van Munster
authors.affiliation: Plant Research International, PO Box 16, 6700 AA Wageningen, The Netherlands.
authors.forename: A M
authors.initials: AM
authors.lastname: Dullemans
authors.affiliation: Plant Research International, PO Box 16, 6700 AA Wageningen, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Verbeek
authors.affiliation: De Ruiter Seeds, PO Box 1050, 2660 BB Bergschenhoek, The Netherlands.
authors.forename: J F J M
authors.initials: JFJM
authors.lastname: van den Heuvel
authors.affiliation: INRA, 28 rue de Herrlisheim, 68021 Colmar Cédex, France.
authors.forename: C
authors.initials: C
authors.lastname: Reinbold
authors.affiliation: INRA, 28 rue de Herrlisheim, 68021 Colmar Cédex, France.
authors.forename: V
authors.initials: V
authors.lastname: Brault
authors.affiliation: Unité de Phytopathologie, IRD, BP 5045 Montpellier, France.
authors.forename: A
authors.initials: A
authors.lastname: Clérivet
authors.affiliation: Plant Research International, PO Box 16, 6700 AA Wageningen, The Netherlands.
authors.forename: F
authors.initials: F
authors.lastname: van der Wilk
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000821:virology*; D001483:Base Sequence; D004267:DNA Viruses / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D017990:Densovirus / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D022821:RNA Splice Sites; D017422:Sequence Analysis, DNA; D014764:Viral Proteins
chemical_list: D022821:RNA Splice Sites; D014764:Viral Proteins