title: [Effect of lipopolysaccharide on recombinant human osteogenic protein-1-induced responses in human dental pulp cells].
abstract: OBJECTIVE
  To investigate the effects of lipopolysaccharide (LPS) on recombinant human osteogenic protein-1 (rhOP-1) induced proliferation and differentiation.
  
  
  METHODS
  Measured MTT, alkaline phosphatase (ALPase) activity and osteocalcin content of human dental pulp cells.
  
  
  RESULTS
  Low-dose LPS (1 microgram/ml) caused co-operative effect with low concentration of rhOP-1 to enhance human dental pulp cell proliferation and ALPase activity, but not to osteocalcin, whereas, high-dose LPS (100 micrograms/ml) significantly inhibited the proliferation, ALPase activity and osteocalcin with or without rhOP-1.
  
  
  CONCLUSIONS
  The role of LPS in caries-induced reparative dentin might be a significant determinant.
authors.affiliation: College of Stomatology, West China University of Medical Sciences.
authors.forename: Q
authors.initials: Q
authors.lastname: Su
authors.forename: G
authors.initials: G
authors.lastname: He
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: Q
authors.initials: Q
authors.lastname: Li
mesh_terms: D000469:Alkaline Phosphatase / Q000187:drug effects; D055395:Bone Morphogenetic Protein 1; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D004357:Drug Synergism; D016967:Fusobacterium nucleatum / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008666:Metalloendopeptidases / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D019485:Bone Morphogenetic Proteins; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D000469:Alkaline Phosphatase; D008666:Metalloendopeptidases; C525705:BMP1 protein, human; D055395:Bone Morphogenetic Protein 1