[Study of the inhibitory effect of PhAsO on gap junctional communication and its damages to cellular DNA of human skin fibroblast cells].
In order to investigate the toxicity of organic arsenic as well as the mechanism of arsenic carcinogenesis, the GJIC between human skin fibroblast cells is detected by the scrape loading/dye transfer assay and single-cell gel electrophoresis(SCGE) assay is used to detect the DNA strand breaks in human skin fibroblast cells exposed to PhAsO. The results show that gap junctional intercellular communication between human skin fibroblast cells is inhibited by PhAsO significantly with a obvious dose-dependent relationship(P < 0.01), suggesting that PhAsO may act as a tumour promoter. PhAsO could induce DNA strand breaks, but the dose-dependent relationship is not observed. The authors suggest that PhAsO may have tumor promoting activity, and further studies are needed.
Deng
Furong
F
School of Public Health, Beijing University, Beijing 100083, China.
Jin
Yu
Y
Wang
Hui
H
Li
Xinhua
X
D001152:Arsenicals / Q000009:adverse effects*; D002450:Cell Communication / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology; D017629:Gap Junctions / Q000187:drug effects*; D006801:Humans; D012867:Skin / Q000166:cytology*
D001152:Arsenicals; C029341:oxophenylarsine