title: Superbeads: immobilization in "sweet" chemistry.
abstract: Enzymatic oligosaccharide synthesis using recombinant glycosyltransferases is able to overcome the difficulties associated with chemical methods. Nonetheless, sugar nucleotide regeneration cycles are necessary for the glycosylation. The multistep enzyme reaction can be efficiently carried out on superbeads that are prepared by immobilizing multienzyme mixtures on bead support through fused binding domains.
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences Dúbravská cesta 9 SK-832 32 Bratislava, Slovak Republic.
authors.forename: Jozef
authors.initials: J
authors.lastname: Nahalka
authors.forename: Ziye
authors.initials: Z
authors.lastname: Liu
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Peng George
authors.initials: PG
authors.lastname: Wang
mesh_terms: D001709:Biotechnology / Q000379:methods; D002236:Carbohydrate Conformation; D002384:Catalysis; D018914:Cells, Immobilized; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D016695:Glycosyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008863:Microspheres; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D009844:Oligosaccharides; D016695:Glycosyltransferases