[Effects of GDNF on primary culture of spinal cord neurons].
The effect of GDNF on long-term cultured spinal cord neurons was studied. GDNF could promote spinal cord neurons survival after 7 d or 14 d culture by MTT assay. The effect of GDNF on growth cones, neuron soma magnitude, neurite length and spines formulation of spinal cord neurons in cell culture was observed by phase microscopy, Nissl stain and NSE immunocytochemistry stain. The results indicated that GDNF had significant trophic effects on long-term cultured spinal cord neurons.
Chen
Z Y
ZY
Department of Neurobiology, Second Military Medical University, Shanghai 200433.
Huang
A J
AJ
Bao
X
X
Lu
C L
CL
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007150:Immunohistochemistry; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D013116:Spinal Cord / Q000166:cytology* / Q000187:drug effects
C495040:Gdnf protein, mouse; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents