[Experimental studies on antitumor effect by the new recombinant tumour necrosis factor and cyclophosphamide in nude mice bearing laryngeal carcinoma].
OBJECTIVE
We aimed at finding ideal biological treatment and regime for laryngeal carcinoma.


METHODS
Nude mice model bearing laryngeal carcinoma were established using laryngeal carcinoma cell line (HEP-2). New recombinant human tumor necrosis factor (NrhTNF-alpha) and cyclophosphamide were administered locally or systemically, single or in combination. The microstructural and ultra-micro-structural changes of carcinoma after administration of NrhTNF-alpha were observed under light and electron microscopes.


RESULTS
1. After treatment, the tumor showed hemorrhage, necrosis, regression, the survival time was prolonged. 2. More significant antitumor effect was observed when locally administered NrhTNF-alpha than intravenously administered of NrhTNF-alpha. 3. There is a notable synergistic antitumor effect by the combination use of NrhTNF-alpha and cyclophosphamide. 4. Microscopy showed that cell changes mostly took place in plasma especially in mitochondria and endoplasmic neticula. The mitochondria were highly bloated. Besides, some nuclei were condensed and fragmented resulted in cell death.


CONCLUSIONS
These studies provide experimental basis for biological treatment of laryngeal carcinoma.
Dong
W
W
Department of Otorhinolaryngology, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029.
Zhou
S
S
Li
Z
Z
D000287:Administration, Topical; D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000008:administration & dosage*; D003520:Cyclophosphamide / Q000008:administration & dosage*; D007822:Laryngeal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011994:Recombinant Proteins / Q000008:administration & dosage; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage*
D018906:Antineoplastic Agents, Alkylating; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D003520:Cyclophosphamide