title: Identification and polymorphism of Plasmodium vivax RBP-1 peptides which bind specifically to reticulocytes.
abstract: Plasmodium vivax merozoite preferentially invades reticulocytes probably using PvRBP-1 as ligand. One hundred and ninety-five, 15-mer peptides has been synthesised from PvRBP-1 sequence; tested in reticulocyte- or erythrocyte-binding assays. Twenty-five peptides (K(d)=76-380 nM) specifically defined four reticulocyte-binding regions. It has been reported that a highly conserved Region-I recombinant fragment binds specifically to reticulocytes. HABP-critical residues for reticulocyte-binding were highly conserved in 20 Colombian P. vivax clinical isolates, suggesting an important biological function. There were six overlapping reticulocyte-binding sites for these peptides according to enzyme sensitivity and mutual competition-binding assays; located on 26- and 41-kDa reticulocyte membrane surface proteins.
authors.affiliation: Fundacion Instituto de Inmunologia de Colombia and Universidad Nacional de Colombia, Cra 50 No 26-00, Bogotá, Colombia SA. mauriciourquiza@yahoo.com
authors.forename: Mauricio
authors.initials: M
authors.lastname: Urquiza
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Patarroyo
authors.forename: Viviana
authors.initials: V
authors.lastname: Marí
authors.forename: Marisol
authors.initials: M
authors.lastname: Ocampo
authors.forename: Jorge
authors.initials: J
authors.lastname: Suarez
authors.forename: Ramses
authors.initials: R
authors.lastname: Lopez
authors.forename: Alvaro
authors.initials: A
authors.lastname: Puentes
authors.forename: Hernando
authors.initials: H
authors.lastname: Curtidor
authors.forename: Javier
authors.initials: J
authors.lastname: García
authors.forename: Luis E
authors.initials: LE
authors.lastname: Rodríuez
authors.forename: Ricardo
authors.initials: R
authors.lastname: Vera
authors.forename: Angela
authors.initials: A
authors.lastname: Torres
authors.forename: Marilu
authors.initials: M
authors.lastname: Laverde
authors.forename: Ana P
authors.initials: AP
authors.lastname: Robles
authors.forename: Manuel E
authors.initials: ME
authors.lastname: Patarroyo
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000235:genetics / Q000502:physiology; D004912:Erythrocytes / Q000378:metabolism; D007700:Kinetics; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D010966:Plasmodium vivax / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D012156:Reticulocytes / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D010455:Peptides; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; C075483:reticulocyte-binding protein, Plasmodium vivax