[Effects of the physical configuration of plasmid DNA and other factors on production of fertile transgenic wheat plants].
The applicability of hyperosmotic treatment and different configurations of plasmid DNA for stable transformation of wheat mediated by particle bombardment was investigated. Hyperosmotic treatment increased the frequency of transient expression and had also a positive effect on stable transformation. Denaturation of plasmid DNA prior to bombardment led to dramatic reduction of transient expression. However, there were no marked differences between single-stranded and double-stranded DNA in stable transformation. Single-stranded plasmid DNA, double-stranded plasmid DNA in linear state and double-stranded plasmid DNA in circular state could all be used to produce transgenic wheat plants. A total of 26 independent transgenic plants of winter wheat genotype Florida and 4 independent transgenic plants of spring wheat genotype Veery were obtained. Most transgenic plants have set seeds. T2 seeds of some spring wheat transgenic plants have also been harvested.
Xu
Z Q
ZQ
Provincial Key Laboratory of Biotechnology, Northwest University, Xian 710069.
Becker
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D
Lörz
H
H
D000613:Aminobutyrates / Q000494:pharmacology; D019470:Biolistics; D005298:Fertility / Q000235:genetics; D009997:Osmotic Pressure; D030821:Plants, Genetically Modified; D010957:Plasmids; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics*
D000613:Aminobutyrates; C003121:phosphinothricin