title: The role of myoepithelial cells in the short-term radiogenic impairment of salivary glands. An immunohistochemical, ultrastructural and scintigraphic study.
abstract: OBJECTIVE
  The aim of this study was to evaluate the early dose-related functional impairment of salivary glands after radiation, using sialoscintigraphy, immunohistochemistry and electron microscopy in an established rabbit experimental model.
  
  
  METHODS
  Twelve rabbits were used for the study. Eight were scintigraphically examined prior to and 24 hours after 15/30 Gy (4 rabbits each). The irradiated glands were examined immunohistochemically using monoclonal antibodies to alpha-smooth muscle actin (ASMA), vimentin and Ki-67 proliferation antigen. Ultrastructural investigation was also performed. Four control rabbits were sham-treated and provided normal salivary gland tissue.
  
  
  RESULTS
  There was a significant increase of the 99mTc-pertechnetate uptake in the irradiated parotid glands (p < 0.05) and a highly significant one in the superficial mandibular glands (p < 0.001). Immunohistochemically a significant loss of ASMA and vimentin-stained myoepthelial cells and a decrease of the proliferating rate in the acinar cells could be assessed in both irradiated glands. Ultrastructurally, rarefaction and focal condensation of the myofilaments of the myoepithelial cells in both irradiated glands was observed. No dose-related effect could be assessed.
  
  
  CONCLUSIONS
  Early functional impairment of salivary glands after radiation could be revealed as early as 24 hours after radiation. The considerable myoepthelial cell impairment may explain the secretory retention assessed scintigraphically and provide -apart from acinar cell damage--a new aspect in the pathogenesis of radiogenic impairment of salivary glands.
authors.affiliation: Department of Maxillofacial Surgery, Medical University of Luebeck, Germany. Samer.Hakim@mkg-chir.mu-luebeck.de
authors.forename: Samer G
authors.initials: SG
authors.lastname: Hakim
authors.forename: Hartwig
authors.initials: H
authors.lastname: Kosmehl
authors.forename: Isabel
authors.initials: I
authors.lastname: Lauer
authors.forename: Roger
authors.initials: R
authors.lastname: Nadrowitz
authors.forename: Thilo
authors.initials: T
authors.lastname: Wedel
authors.forename: Peter
authors.initials: P
authors.lastname: Sieg
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D004307:Dose-Response Relationship, Radiation; D004847:Epithelial Cells / Q000502:physiology / Q000528:radiation effects; D005260:Female; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008854:Microscopy, Electron; D010306:Parotid Gland / Q000000981:diagnostic imaging / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D011817:Rabbits; D011833:Radiation Injuries, Experimental / Q000378:metabolism / Q000473:pathology*; D011877:Radionuclide Imaging; D012469:Salivary Glands / Q000000981:diagnostic imaging / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D013670:Sodium Pertechnetate Tc 99m / Q000493:pharmacokinetics; D014746:Vimentin / Q000378:metabolism
chemical_list: D000199:Actins; D019394:Ki-67 Antigen; D014746:Vimentin; D013670:Sodium Pertechnetate Tc 99m