[Characterization of cry gene and broad spectrum against lepidopteran of Bacillus thuringiensis subsp. colmeri 15A3].
Bacillus thuringiensis wild type strain 15A3 belongs to subspecies colmeri serotype H-21. RFLP and PCR analysis show that it contains six types of ICP genes: cry1Aa, cry1Ac, cry1Ca, cry1D, cry1I and cry. The sequence of the 1.45 kb N-terminal fragment of cry1Aa differed from that of published. SDS-PAGE showed that the crystal consists of proteins with molecular weight about 130, 79, 70, 65, 51 and 45 kD. Strain 15A3 didn't sysnthesize heat-stable beta-exotoxins according to test of house fly aberration. The 1.2 tons fermentative production exhibited high toxicity against three lepidopteran pests: H. armigera, S. exigua and H. cunea. It was proved that wild type strain can produce a broad specturm of ICP.
Chen
Yuehua
Y
Nankai University College of Life Sciences, Tain Jin 300071, China. yhchen@nankai.edu.cn
Ren
Gaixin
G
Wu
Weihui
W
Wang
Jinhong
J
Liu
Chunyong
C
D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D002869:Chromosome Aberrations; D004731:Endotoxins / Q000235:genetics*; D005098:Exotoxins / Q000032:analysis; D005285:Fermentation; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D006793:Houseflies / Q000235:genetics; D007915:Lepidoptera / Q000235:genetics; D010572:Pest Control, Biological
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D005098:Exotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis