Abnormal production of interleukin (IL)-11 and IL-8 in polycythaemia vera.
We studied the production of interleukin (IL)-11 and IL-8, two cytokines known to affect erythropoiesis, in polycythemia vera (PV). In vivo, IL-11 was detected more frequently in serum and bone marrow (BM) plasma of PV patients than in controls (healthy donors and patients with idiopathic erythrocytosis (IE)). In addition, serum IL-11 levels of PV patients were higher than those of controls. IL-8 was elevated in serum of both PV and IE patients (respective median levels: 38.6 and 242pg/ml, vs 4.4pg/ml for healthy donors). BM plasma IL-8 levels of PV patients (508pg/ml) were significantly higher than those of IE patients (120pg/ml). In vitro, bone marrow (BM) stromal cells (BMSC) of PV patients produced significantly more IL-11 (x6.4) and IL-8 (x8.3) than BMSC of healthy donors or IE patients. In conclusion, both IL-11 and IL-8 are overproduced in PV, apparently by BMSC; IL-8 is also overproduced in IE, by cells other than BMSC.
Hermouet
Sylvie
S
Laboratoire d'Hématologie et Groupe Cytokines, Récepteurs et Transduction de Signal, Institut de Biologie des Hôpitaux de Nantes, 9 Quai Moncousu, 44093 Nantes Cedex 01, France. Sylvie.Hermouet@nantes.inserm.fr
Godard
Anne
A
Pineau
Danielle
D
Corre
Isabelle
I
Raher
Sylvie
S
Lippert
Eric
E
Jacques
Yannick
Y
D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D017370:Interleukin-11 / Q000096:biosynthesis* / Q000097:blood; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000097:blood; D011086:Polycythemia / Q000097:blood; D011087:Polycythemia Vera / Q000097:blood*; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism
D007375:Interleukin-1; D017370:Interleukin-11; D016209:Interleukin-8