Determination of chromium in cerebrospinal fluid using electrothermal atomisation atomic absorption spectrometry.
A rapid method to determine the chromium content of cerebrospinal fluid (CSF) samples using electrothermal atomization atomic absorption spectrometry (ETA-AAS) with deuterium-arc background correction is described. The chromium concentration in CSF was evaluated by the standard addition method. Sample dilution (1 + 1) with 0.25% (m/v) Triton X-100 and 4.5% (v/v) HNO3 gave the best combination of sensitivity, reproducibility, and low blank reading compared with dilution using other solvents. Within-batch reproducibility was 3.2% for 20 CSF samples, between-batch reproducibility was 4.7%. CSF samples from 43 healthy volunteers collected in a manner designed to avoid contamination yielded chromium concentrations of 14.6 +/- 6.3 ng mL(-1).
Aguilar
María Victorina
MV
Depto. Nutrición y Bromatología, Facultad de Farmacia, Universidad de Alcalá de Henares, Madrid, Spain. mvictorina.aguilar@uah.es
Mateos
Carmen José
CJ
Martínez Para
María Carmen
MC
D002138:Calibration; D002857:Chromium / Q000134:cerebrospinal fluid*; D006358:Hot Temperature; D006801:Humans; D012680:Sensitivity and Specificity; D012997:Solvents; D013054:Spectrophotometry, Atomic / Q000379:methods*
D012997:Solvents; D002857:Chromium