[Forensic application of reverse dot blot with biotin incorporation].
A rapid and accurate reverse dot blot technique was successfully established. It could distinguish the 6 alleles of 0101, 0102, 0103, 0201, 0301 and 0401 of HLA-DQA locus. Alleles and genotype frequencies for HLA-DQA locus were determined in 200 unrelated individuals of northern Han population. The discrimination power (DP) was 0.928, heterozygocity (H) 0.75. By direct biotin incorperation, 1 ng genome DNA could be detected successfully. This method can be used for paternity test and individual identification in forensic science practice.
Jiang
W B
WB
Forensic Science Institute of Liaoning Province, Shenyang 110032.
Jiang
X H
XH
D000483:Alleles; D001710:Biotin; D004247:DNA / Q000032:analysis*; D005554:Forensic Medicine / Q000379:methods*; D005787:Gene Frequency; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D001710:Biotin; D004247:DNA