Use of adenovirus vectors for functional gene analysis in the chicken chorioallantoic membrane.
The chicken chorioallantoic membrane (CAM) assay represents one of the most widely used in vivo screening assay for genes with angiogenic (blood vessel-inducing) or angiostatic (inhibition of vessel formation or their destruction) activities. Here we show that adenovirus gene transfer vectors infect cells in the CAM and lead to expression of the viral transgene. Furthermore, infection with an adenovirus vector containing the human vascular endothelial growth factor gene induced the formation of new blood vessels. This improved method saves a considerable amount of time in the identification of genes that can influence blood vessel formation because the expensive and time-consuming production and purification of recombinant protein can be omitted.
Schughart
Klaus
K
GBF, German Research Center for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany. kls@gbf.de
Accart
Nathalie
N
D000260:Adenoviruses, Human / Q000235:genetics*; D000482:Allantois / Q000098:blood supply / Q000166:cytology / Q000502:physiology*; D043925:Angiogenesis Inducing Agents / Q000235:genetics / Q000502:physiology; D020533:Angiogenesis Inhibitors; D000818:Animals; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply / Q000166:cytology / Q000502:physiology*; D005240:Feasibility Studies; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology; D005822:Genetic Vectors; D006801:Humans; D007763:Lac Operon / Q000235:genetics; D018919:Neovascularization, Physiologic / Q000235:genetics* / Q000502:physiology; D014162:Transfection / Q000379:methods*; D019076:Transgenes; D042461:Vascular Endothelial Growth Factor A
D043925:Angiogenesis Inducing Agents; D020533:Angiogenesis Inhibitors; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A