[The morphological structure of classical swine fever virus and some characteristics of its multiplication].
Some characteristics of the multiplication of classical swine fever virus(CSFV) Thiverval strain were studied by means of the immunofluorescence technique. Under optimum culture conditions, the concentration of CSFV in the culture liquid multiplicated by MPK cells is ten times higher than by PK-15 cells. The half-life period of CSFV at 37 degrees C is 3 hour. The location of CSFV's replication in host cells is detected by the monoclonal antibodies of the structural protein E2 and the non-structural protein p120 of CSFV. Based on the above results, the ultrastructure of CSFV particles inside host cells was further studied using electron microscope and the changes of the ultrastructure of CSFV-infected cells are shown. The possible reasons for lower viral titre are also discussed in this paper.
Wang
Z
Z
College of Life Sciences, Peking University, Beijing 100871.
Lu
Y
Y
Zhou
P
P
Zhai
Z
Z
Ding
M
M
D000818:Animals; D002460:Cell Line; D006692:Classical Swine Fever Virus / Q000502:physiology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006207:Half-Life; D008854:Microscopy, Electron; D013552:Swine; D013696:Temperature; D014765:Viral Vaccines; D014776:Virus Cultivation; D014779:Virus Replication
D014765:Viral Vaccines