Quantitative HRMAS proton total correlation spectroscopy applied to cultured melanoma cells treated by chloroethyl nitrosourea: demonstration of phospholipid metabolism alterations.
Recent NMR spectroscopy developments, such as high-resolution magic angle spinning (HRMAS) probes and correlation-enhanced 2D sequences, now allow improved investigations of phospholipid (Plp) metabolism. Using these modalities we previously demonstrated that a mouse-bearing melanoma tumor responded to chloroethyl nitrosourea (CENU) treatment in vivo by altering its Plp metabolism. The aims of the present study were to investigate whether HRMAS proton total correlation spectroscopy (TOCSY) could be used as a quantitative technique to probe Plp metabolism, and to determine the Plp metabolism response of cultured B16 melanoma cells to CENU treatment in vitro. The exploited TOCSY signals of Plp derivatives arose from scalar coupling among the protons of neighbor methylene groups within base headgroups (choline and ethanolamine). For strongly expressed Plp derivatives, TOCSY signals were compared to saturation recovery signals and demonstrated a linear relationship. HRMAS proton TOCSY was thus used to provide concentrations of Plp derivatives during long-term follow-up of CENU-treated cell cultures. Strong Plp metabolism alteration was observed in treated cultured cells in vitro involving a down-regulation of phosphocholine, and a dramatic and irreversible increase of phosphoethanolamine. These findings are discussed in relation to previous in vivo data, and to Plp metabolism enzymatic involvement.
Morvan
Daniel
D
Institut National de la Santé et de la Recherche Médicale U484, Clermont-Ferrand, France. morvan@inserm484.u-clermont.fr
Demidem
Aicha
A
Papon
Janine
J
Madelmont
Jean Claude
JC
D000818:Animals; D003543:Cysteamine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008546:Melanoma, Experimental / Q000378:metabolism*; D051379:Mice; D009607:Nitrosourea Compounds / Q000494:pharmacology*; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism
D009607:Nitrosourea Compounds; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D003543:Cysteamine; C051262:N'-(2-chloroethyl)-N-(2-(methylsulfinyl)ethyl)-N'-nitrosourea