[Chiral separation of thio-glycidyl ether on amylose-chiral stationary phase by high performance liquid chromatography].
In the present study, we synthesized amylose-tris(phenylcarbamate), and used it as chiral stationary phase based on silica gel. This stationary phase offered a practically useful chiral separation medium. Amylose was allowed to react with an excess of phenylisocyanate in pyridine for 48 h. The obtained carbamate was dissolved in DMF and adsorbed on silica gel previously treated with 3-aminopropyltriethoxysilane. The mass ratio of the carbamate to the silica gel was 0.45/2.55. The four thio-glycidyl ethers were resolved on the amylose tris(phenylcarbamate) chiral stationary phase. Separation was carried out with a hexane/2-propanol mixture as mobile phase at a flow rate of 1.0 mL/min at room temperature. The effects of 2-propanol concentration on the retention and resolution have been investigated.
Zhou
Z
Z
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000.
Yu
Z
Z
Niu
T
T
Jiang
S
S
Chen
L
L
D000688:Amylose / Q000031:analogs & derivatives*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004852:Epoxy Compounds / Q000737:chemistry / Q000302:isolation & purification*; D058428:Silica Gel; D012822:Silicon Dioxide; D013237:Stereoisomerism
D004852:Epoxy Compounds; C106151:amylose tris(1-phenylethylcarbamate); C020344:glycidyl ethers; D058428:Silica Gel; D012822:Silicon Dioxide; D000688:Amylose