[Fermentation conditions for production of alkaline elastase by alkaliphilic Bacillus XE22-4-1].
A extracellular alkaline elastase producing bacterial strain Bacillus XE22-4-1, with the optimum pH of 10.0 for its growth, was isolated and screened from alkali lake of Tibet. 2% glucose and 0.25% yeast extract are respectively the appropriate carbon and nitrogen source on elastase production. Soymeal can promote elastase production. The fermentation conditions in a 2 L stirred tank were investigated. The results have revealed that the dissolved oxygen is the most effective factor on elastase production. The maximum elastolytic activity reached 266 u/mL among 48 hours of fermentation by combination of enhancing aeration and changing the mixing speed.
Xiao
C
C
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Lu
J
J
Tian
X
X
Li
X
X
Zhou
P
P
D001407:Bacillus / Q000201:enzymology* / Q000302:isolation & purification; D004795:Enzyme Stability; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000378:metabolism; D010196:Pancreatic Elastase / Q000096:biosynthesis* / Q000302:isolation & purification; D013696:Temperature; D014871:Water Microbiology
C042629:alkaline elastase; D010196:Pancreatic Elastase; D005947:Glucose; D010100:Oxygen