title: Enhanced expression of the UROC28 gene in human breast cancer: relationship to ERBB2 gene expression.
abstract: BACKGROUND
  ERBB2, a highly important oncogene in invasive breast cancer, is not only a prognostic factor but also a predictive marker for response to therapeutic agents. Recently, An et al. (10) identified a novel gene, UROC28, that is also overexpressed in breast cancer. To examine possible interrelationships, we quantitated UROC28 and ERBB2 mRNA in breast cancers.
  
  
  METHODS
  The expression of UROC28 and ERBB2 mRNA in breast cancer tissues were determined using RT-PCR. The expression was also examined in T-47D breast cancer cells treated with estrogen.
  
  
  RESULTS
  UROC28 mRNA expression was greater in cancers than in noncancerous tissues (p < 0.0001), as was ERBB2 mRNA. ERBB2 and UROC28 gene expression was dose-dependently down-regulated in T-47D breast cancer cells treated with estradiol. However UROC28 mRNA and ERBB2 mRNA expression did not correlate with one another.
  
  
  CONCLUSIONS
  These results indicate that UROC28 may be a useful target molecule in breast cancer diagnosis and treatment, complementing ERBB2.
authors.affiliation: Department of Laboratory Diagnosis and Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo, 060-8543, Japan.
authors.forename: Chinatsu
authors.initials: C
authors.lastname: Kamagata
authors.forename: Naoki
authors.initials: N
authors.lastname: Tsuji
authors.forename: Kei
authors.initials: K
authors.lastname: Kondoh
authors.forename: Masateru
authors.initials: M
authors.lastname: Sasaki
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kobayashi
authors.forename: Atsuhito
authors.initials: A
authors.lastname: Yagihashi
authors.forename: Naoki
authors.initials: N
authors.lastname: Watanabe
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D018734:Genes, erbB-2 / Q000235:genetics*; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D004958:Estradiol; D009363:Neoplasm Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; C420220:PBOV1 protein, human