[Strain screening and fermentation conditions of a novel heparinase-producing strain].
The novel heparinase-producing bacterial strain Corynebacterium sp. was screened and isolated from soil. The optimum medium composition is (g/L): Trypticase 20, NaCl 1, K2HPO4 2.5, MgSO4 0.5, Heparin 2, maltose 20, pH 6.5. The optimum growth temperature was 27 degrees C while, maximum enzyme production was achieved at temperature 31 degrees C. When cultured at a rotating shaker at 30 degrees C for 24 hours, 200 r/min, 40 mL medium in 500 mL flask, the Production of heparinase reached 1700 u/L.
Gao
N
N
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
Cheng
X
X
Yang
J
J
Zhang
S
S
D003352:Corynebacterium / Q000201:enzymology* / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D019763:Heparin Lyase / Q000096:biosynthesis* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D013696:Temperature
D003470:Culture Media; D019763:Heparin Lyase