title: Specific stimulation of MHC-transgenic mouse T-cell hybridomas with xenogeneic APC.
abstract: From the recombinant human leukocyte antigen (HLA)-DR1/H2-E(k) major histocompatibility complex (MHC) class II-transgenic mice, we have generated two CD4(+) T-cell hybridomas specific for peptides which were derived from human prostatic acid phosphatase (PAP) complexed to the human class II molecule HLA-DR1. Both hybridomas strongly react to PAP-pulsed antigen-presenting cells (APC) from transgenic mice. Interestingly, these hybridomas also responded to PAP antigen presented by HLA-DR1-positive human APC. The species-mismatched T-cell stimulation occurs despite the biologic discordance in participating accessory molecules, which are required for the optimal T-cell-APC interaction. Our results demonstrate various degrees of functional interaction between coreceptors, costimulatory molecules, and integrins, which are expressed on the surface of T-cell hybridomas and heterologous APC.
authors.affiliation: Dendreon Corporation, Seattle, WA 98121, USA. dvidovic@dendreon.com
authors.forename: Damir
authors.initials: D
authors.lastname: Vidovic
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Graddis
authors.forename: Lara P
authors.initials: LP
authors.lastname: Stepan
authors.forename: Dennis M
authors.initials: DM
authors.lastname: Zaller
authors.forename: Reiner
authors.initials: R
authors.lastname: Laus
mesh_terms: D000135:Acid Phosphatase; D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology*; D015478:Antigens, Heterophile / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000276:immunology; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D015798:HLA-DR1 Antigen / Q000235:genetics / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D017027:Protein Tyrosine Phosphatases / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D013045:Species Specificity
chemical_list: D015478:Antigens, Heterophile; D018984:Epitopes, T-Lymphocyte; D006183:H-2 Antigens; C068318:H-2E(k) antigen; D015798:HLA-DR1 Antigen; D007376:Interleukin-2; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D000135:Acid Phosphatase; C428439:prostatic acid phosphatase; D017027:Protein Tyrosine Phosphatases