Mechanical ventilation-induced pneumoprotein CC-16 vascular transfer in rats: effect of KGF pretreatment.
After air-blood barrier injury, "pneumoproteins" specific to lung epithelial distal airspaces reaching the bloodstream are putative markers of lung hyperpermeability. The contribution of mechanical ventilation (MV) to this leakage is unknown. To explore this issue, 16-kDa Clara cell protein (CC-16) concentration was quantified in bronchoalveolar lavages (BALFs) and/or sera of rats first exposed either to ambient air or to 48 h of hyperoxia-induced acute lung injury and then ventilated for 2 h according to one of the following strategies: 1) spontaneous ventilation (SV), 2) very-low-volume high PEEP (VLVHP, where PEEP is positive end-expiratory pressure), 3) low-volume zero PEEP, 4) moderate-volume low PEEP, and 5) high-volume zero PEEP (HVZP). Results show that total proteins in BALFs increased with time and MV, with little impact from hyperoxia preexposure. CC-16 content decreased in BALFs but increased in the bloodstream during MV, suggesting intravascular leakage. Lung overdistension may result either from high-volume (HVZP) or high-PEEP (VLVHP) MV, and it was the most potent inducer of CC-16 leakage (P < 0.05 vs. SV). In the VLVHP group, pretreatment with keratinocyte growth factor was efficient in reducing blood CC-16 transfer.
Lesur
Olivier
O
Groupe de Recherche en Physiopathologie Respiratoire et Unité des Soins Intensifs Médicaux, Département de Médecine, Centre Hospitalier Universitaire de Sherbrooke, Quebec, Canada J1H 5N4. Olivier.Lesur@USherbrooke.ca
Hermans
Cédric
C
Chalifour
Jean-François
JF
Picotte
Johanne
J
Lévy
Bruno
B
Bernard
Alfred
A
Lane
Denis
D
D000818:Animals; D001769:Blood / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002199:Capillary Permeability / Q000187:drug effects* / Q000502:physiology*; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D018496:Hyperoxia / Q000150:complications; D008168:Lung / Q000378:metabolism*; D008171:Lung Diseases / Q000503:physiopathology; D008297:Male; D009994:Osmolar Concentration; D011175:Positive-Pressure Respiration / Q000379:methods*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013997:Time Factors; D014598:Uteroglobin
C496455:Fgf7 protein, rat; D011506:Proteins; C556391:Scgb1a1 protein, rat; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D014598:Uteroglobin