[The gene expression of some cytokines and collagen proteins in rat bone tissue is related to estradiol (E2) and age].
30 female SD rats (3 months old) are equally divided into three groups: ovariectomy (OVX) rats, sham-operated (SHO) rats and 17 beta estradiol (E2) treated OVX rats. For each group, mRNA was isolated from long bone at one month and three months after surgery, respectively. mRNA was reverse transcribed into single strand cDNA and then used as a probe hybridizing to the DNA fragments of col I alpha(1), col I alpha(2), col III, col V, fibronectin, IL-1, IL-6, TGF-beta, LIF, TNF-alpha, TNF-beta by reverse northern and dot blot hybrization. The housekeeping gene, gapdh, was used as an internal control. The results show that in bone of rat, the stable expression of col I alpha (1), col I alpha(2) and col III are related to age not ovariectomy, while supplement with E2 can inhibit the expression of col III and col I alpha(2) completely. The expression of col V, IL-1, IL-6 can be inhibited by estrogen and recovered by removal of estrogen by OVX, then addition of E2 decreased it to the normal level. The expression of TGF-beta is also inhibited by estrogen. It increased during one month after overiectomy, and partially decreased in E2 complemented rat. Three months after surgery, the level of increasing and decreasing is less evident as two months ago. It seems that in young SD rat, the expression of TGF-beta is related to both estrogen and age.
Huang
H N
HN
Institute of Genetics, Fudan University, Shanghai 200433.
Tu
Z C
ZC
Liao
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Zhao
S Y
SY
Li
C B
CB
Huang
S Z
SZ
Tang
T T
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Dai
K R
KR
D000367:Age Factors; D000818:Animals; D003094:Collagen / Q000096:biosynthesis*; D016207:Cytokines / Q000096:biosynthesis*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005269:Femur / Q000378:metabolism*; D015870:Gene Expression; D010052:Ovariectomy; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
D016207:Cytokines; D012333:RNA, Messenger; D004958:Estradiol; D003094:Collagen