Semi-micro high-performance liquid chromatographic analysis of tiropramide in human plasma using column-switching.
A rapid and sensitive column-switching semi-micro high-performance liquid chromatography method was developed for the direct analysis of tiropramide in human plasma. The plasma sample (100 microl) was directly injected onto Capcell Pak MF Ph-1 precolumn where deproteinization and analyte fractionation occurred. Tiropramide was then eluted into an enrichment column (Capcell Pak UG C(18)) using acetonitrile-potassium phosphate (pH 7.0, 50 mM) (12:88, v/v) and was analyzed on a semi-micro C(18) analytical column using acetonitrile-potassium phosphate (pH 7.0, 10 mM) (50:50, v/v). The method showed excellent sensitivity (limit of quantification 5 ng/ml), and good precision (C.V.<or=5.9%), and speed (total analysis time 20 min). The calibration curve was linear in the concentration range 5-200 ng/ml (coefficient of determination equal to 0.998). This method was successfully applied to the pharmacokinetic study of tiropramide in volunteers.
Baek
Soo Kyoung
SK
College of Pharmacy and Medicinal Resources Research Center, Wonkwang University, Shinyongdong, Iksan 570-749, South Korea.
Lee
Seung Seok
SS
Park
Eun Jeon
EJ
Sohn
Dong Hwan
DH
Lee
Hye Suk
HS
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D006801:Humans; D008297:Male; D010276:Parasympatholytics / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D013607:Tablets; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics
D010276:Parasympatholytics; D013607:Tablets; D014443:Tyrosine; C031173:tiropramide