Range of separation of potential tool for bioseparation, microchip electrophoresis system, for DNA polymorphisms on the human Y-chromosome.
For the requirement of a high, fast and sufficient technology to suit the needs of 21st century biotechnology, the separation range of a microchip electrophoresis system was studied. Two DNA fragments on the human Y-chromosome, SY594 (82 bp) and 12f2 (88 bp), were successfully separated with a reproducibility of 1.9% and an accuracy of 2.8%. Then, a mixture of 10 DNA markers ranging from 61 bp to 189 bp was successfully separated with high resolution. All of these results demonstrate the superiority of microchip electrophoresis as a tool for 21st century bioseparation.
Jabasini
Mohammad
M
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, CREST, Japan Science and Technology Corporation, Tokushima 770-8505, Japan. c400141005@stud.tokushima-u.ac.jp
Xu
Feng
F
Dang
Fuquan
F
Shinka
Toshikatsu
T
Nakahori
Yutaka
Y
Baba
Yoshinobu
Y
D041322:Chromosomes, Human, Y; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008904:Miniaturization; D011110:Polymorphism, Genetic