Vaccination against type F botulinum toxin using attenuated Salmonella enterica var Typhimurium strains expressing the BoNT/F H(C) fragment.
The utility of the htrA, pagC and nirB promoters to direct the expression of the carboxy-terminal (H(C)) fragment of botulinum toxin F (FH(C)) in Salmonella enterica var Typhimurium has been evaluated. Only low levels of serum antibody were induced after immunisation, and some protection against botulinum toxin type F was demonstrated after oral immunisation of mice with two doses of any of these recombinant Salmonella. Immunisation with two doses of recombinant Salmonella expressing FH(C) from the htrA promoter gave the greatest protection, against up to 10,000 mouse lethal doses of botulinum toxin type F. These results demonstrate the feasibility of an orally delivered vaccine against botulinum toxin type F.
Foynes
Susan
S
Department of Biological Sciences, Centre for Molecular Microbiology, Imperial College for Science, Technology and Medicine, Exhibition Road, London SW7 2AZ, UK.
Holley
Jane L
JL
Garmory
Helen S
HS
Titball
Richard W
RW
Fairweather
Neil F
NF
D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000235:genetics; D001428:Bacterial Vaccines / Q000008:administration & dosage; D001905:Botulinum Toxins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D001906:Botulism / Q000517:prevention & control*; D003014:Clostridium botulinum / Q000276:immunology*; D005240:Feasibility Studies; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D005813:Genes, Synthetic; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D006360:Heat-Shock Proteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D033903:Periplasmic Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D012486:Salmonella typhimurium / Q000235:genetics*; D012697:Serine Endopeptidases / Q000235:genetics; D014611:Vaccination; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic / Q000276:immunology
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; C478239:NirB protein, Salmonella typhimurium; D010446:Peptide Fragments; D033903:Periplasmic Proteins; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic; C066766:pagC protein, Salmonella typhimurium; C061682:DegP protease; D012697:Serine Endopeptidases; D001905:Botulinum Toxins; C096321:botulinum toxin type F