Cloning and characterization of the promoter region of human spata2 (spermatogenesis-associated protein 2) gene.
In this paper, we report the genomic organization of the human spata2 (spermatogenesis-associated protein 2) gene and the characterization of its promoter region. The 5'-flanking region lacks canonical TATA-box but contains a CAAT-box and potential binding sites for a variety of transcription factors such as Sp1, NF-1 and CREB. The results indicated that (1) the cis-acting elements necessary for a basal transcription of spata2 are contained within the -81/+159 proximal sequence of the promoter and (2) the strongest promoter activity was exhibited by the -403/+159 region that contains a CREB site.
Liliana Slongo
M
M
Department of Experimental Biomedical Sciences, University of Padova, Viale G. Colombo 3, 35121 Padua, Italy.
Zotti
Lorenza
L
Onisto
Maurizio
M
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D003001:Cloning, Molecular; D017362:Cyclic AMP Response Element-Binding Protein / Q000737:chemistry; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000502:physiology*; D011506:Proteins / Q000235:genetics*; D024363:Transcription Initiation Site
D017362:Cyclic AMP Response Element-Binding Protein; D018076:DNA, Complementary; D011506:Proteins; C425523:SPATA2 protein, human