Downregulation of mafB expression in T-helper cells during early differentiation in vitro.
We have studied the expression of a human homologue of mafB (maf-1), a member of the family of large maf transcription factors. In support of the suggested key role that mafB expression plays in differentiating macrophages, we found mafB to be expressed at a very high level in monocytic U937 and THP-1 cell lines. However, we show here that mafB transcription is not restricted to myeloid cells but can also be detected in lymphoid cells, indicating transcriptional plasticity during haematopoiesis. In conclusion, strong proliferative signals mediated by T-cell activation and interleukins (IL-4 and IL-12) downregulate the mafB messenger RNA transcript level when resting naïve CD4+ T-helper cells enter the differentiation pathway in vitro.
Hashizume
H
H
Inflammatory Disease Unit, Roche Bioscience, Palo Alto, CA, USA.
Hamalainen
H
H
Sun
Q
Q
Sucharczuk
A
A
Lahesmaa
R
R
D030161:Avian Proteins; D002454:Cell Differentiation; D002460:Cell Line; D004268:DNA-Binding Proteins; D015536:Down-Regulation; D006801:Humans; D051258:MafB Transcription Factor; D015513:Oncogene Proteins / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors
D030161:Avian Proteins; D004268:DNA-Binding Proteins; C490407:MAFB protein, human; D051258:MafB Transcription Factor; D015513:Oncogene Proteins; D015534:Trans-Activators; D014157:Transcription Factors