[Breeding of yeast for beer manufacturing by inactivated protoplast fusion].
QSB-XI6, one of fusants, was obtained by PEG-induced fusion between UV-inactivated protoplasts of Saccharomyces cerevisiae LQ16 (Ile-, Datr) and heat-inactivated protoplasts of S. cerevisiae QSB7(Ala-, H2S-). The cell volume, bicmass, genotype and DNA content of the fusant were measured in comparison with of its parents and the results showed that QSB-XI6 was a fusant. The fermentation ability and the flocculent capacity of this fusant were higher than those of its parents. The diacetyl content of the beer produced by the fusant was measured and some other compositions of the beer was also analysed by gas chromatography. The sensory evaluation of the beer was much better and the flavour was distinctive. The results of many production trials in succession showed that all good characteristics of QSB-XI6 were stable.
Zhou
D
D
Biology Department of Teacher's College of Qiqihar University, Qiqihar 161006.
Ping
W
W
Sun
J
J
Zhang
B
B
Wang
C
C
Guo
X
X
D002459:Cell Fusion; D004271:DNA, Fungal / Q000032:analysis; D005838:Genotype; D007218:Industrial Microbiology / Q000379:methods*; D011523:Protoplasts; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology
D004271:DNA, Fungal