title: Synthesis of oligonucleotides with a 2'-cap at the 3'-terminus via reversed phosphoramidites.
abstract: [reaction: see text] A method is presented for the synthesis of single compounds or small combinatorial libraries of oligonucleotides with 2'-acylamido-2'-deoxyuridine residues at the 3'-terminus. Selection experiments identified the residue of anthraquinone-2-carboxylic acid as a "molecular cap" that increases the UV melting point of the duplex (5'-ACGCGU-3')(2) by up to 28 degrees C compared to the unmodified control duplex.
authors.affiliation: Department of Chemistry, University of Constance, 78457 Konstanz, Germany.
authors.forename: William H
authors.initials: WH
authors.lastname: Connors
authors.forename: Sukunath
authors.initials: S
authors.lastname: Narayanan
authors.forename: Olga P
authors.initials: OP
authors.lastname: Kryatova
authors.forename: Clemens
authors.initials: C
authors.lastname: Richert
mesh_terms: D001483:Base Sequence; D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000235:genetics; D009943:Organophosphorus Compounds / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D004247:DNA