[Expression of a HCV multi-epitopes antigen gene and study on its immunogenicity].
Due to the hypervariable character of hepatitis C virus (HCV), 5 conserved T and/or B cell epitopes from core, envelope, NS3 and NS5 protein of HCV were chosen to form a 270 bp multi-epitopes antigen gene. The gene was clone into a fusion vector pWR450-1 to express a beta-galactosidase-HCV hybrid protein GZ-PCX. The purified GZ-PCX protein was specifically recognized by human anti-HCV antibodies. These results show that the HCV hybrid multi-epitopes antigen has excellent immunogenicity, which might be able to be used as an effective diagnosis agent and to provide protectivity to any genotype of HCV which might partly solve the problems in the researches of HCV vaccines.
Huang
J
J
State Key Laboratory, Institute of Genetics, Fudan University, Shanghai 200433.
Xie
Y
Y
Lin
Y
Y
Ke
S
S
Ren
D
D
D000939:Epitopes / Q000276:immunology; D015870:Gene Expression; D016174:Hepacivirus / Q000276:immunology / Q000378:metabolism*; D018937:Hepatitis C Antibodies / Q000276:immunology*; D018936:Hepatitis C Antigens / Q000096:biosynthesis / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000276:immunology
D000939:Epitopes; D018937:Hepatitis C Antibodies; D018936:Hepatitis C Antigens; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase