[Study on quantitative test on the DNA damage of Aspergillus flavus caused by citral with a comet analysis system].
Comet assay provided a object method for DNA damage caused by physical and chemical factors. A new system of SCGE image analysis, which combines microphotodensitometry and microphotography with image analysis have been developed. Compared with the international popular, it has well at speed, convenience, exchange face of Chinese and English and print. We used it to directly determine the A. flavus' DNA damages for citral. The results show that test time was shorted 2/3 and accuracy was improved. It is probable that cell structural parameter, cell molecule and membrane could be test long, successive, movably and instantly. So the new system has a wide application.
Luo
Man
M
Department of Biological Engineering, Anhui Agriculture University, Hefei 230036, China.
Huang
Yaoxiong
Y
Jiang
Like
L
Ji
Tao
T
Tu
Mei
M
D000080462:Acyclic Monoterpenes; D001231:Aspergillus flavus / Q000235:genetics*; D020552:Comet Assay / Q000295:instrumentation / Q000379:methods*; D004249:DNA Damage; D004269:DNA, Bacterial / Q000187:drug effects*; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods; D039821:Monoterpenes / Q000633:toxicity*
D000080462:Acyclic Monoterpenes; D004269:DNA, Bacterial; D039821:Monoterpenes; C007076:citral