Inorganic plasma with physiological CO2/HCO3- buffer.
The substitution of tris/HCl buffer by CO(2)/HCO(3)(-) buffer in inorganic plasma was studied. An appropriate gas mixture of CO(2)/N(2) was continuously bubbled in Kokubo's SBF solution prepared without addition of Tris/HCl. This method enables buffering the solution within the 7.3-7.4 pH interval and, at the same time, reaching a HCO(3)(-) concentration between 24 and 27 mmol dm(-3), which are the normal concentrations reported for blood plasma. Mineralisation studies on calcium phosphate ceramics using this solution showed that, in the presence of such hydrogencarbonate concentrations, the formation of a mineralisation layer on the ceramic occurs via a carbonated octacalcium phosphate, that evolves to carbonated hydroxyapatite. The results suggest that mineralisation studies in this new carbonate-containing simulated inorganic plasma mimic biomineralisation more closely than traditional SBF.
Marques
P A A P
PA
Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal. paulam@dq.ua.pt
Magalhães
M C F
MC
Correia
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RN
D001639:Bicarbonates / Q000737:chemistry*; D001802:Blood Substitutes / Q000737:chemistry*; D002021:Buffers; D002245:Carbon Dioxide / Q000737:chemistry*; D002516:Ceramics; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
D001639:Bicarbonates; D001802:Blood Substitutes; D002021:Buffers; D002245:Carbon Dioxide