Differential receptors create patterns diagnostic for ATP and GTP.
Herein we report the combination of a library of resin-bound sensors along with a multicomponent sensor array. This novel combinatorial array sensor system shows selectivity for nucleotide phosphates in solution. The design of the anchored receptor includes a 1,3,5-trisubstituted-2,4,6-triethylbenzene scaffold coupled with peptide libraries. Each chemosensor is placed into a micromachined cavity within a silicon wafer, and the optical changes observed by a charged-coupled device result in near-real-time digital analysis of solutions. A colorimetric displacement assay was performed, and time-dependent imaging studies of the selected sensing ensembles result in a differential responses upon addition of adenosine 5'-triphosphate (ATP), adenosine 5'-monophosphate (AMP), or guanosine 5'-triphosphate (GTP). An advantage to this approach is that it creates an array of sensors that gives a fingerprint response for each analyte. Principal component analysis indicates that the library of chemosensors can differentiate between ATP, GTP, and AMP. On the basis of factor loading values, individual sensors from the library were sequenced to elucidate their chemical composition.
McCleskey
Shawn C
SC
University of Texas at Austin, Department of Chemistry and Biochemistry, Austin, Texas 78712, USA.
Griffin
Michael J
MJ
Schneider
Stephen E
SE
McDevitt
John T
JT
Anslyn
Eric V
EV
D000255:Adenosine Triphosphate / Q000032:analysis*; D040761:Biomimetic Materials / Q000737:chemistry; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D019791:Guanidine / Q000737:chemistry*; D006160:Guanosine Triphosphate / Q000032:analysis*; D007700:Kinetics; D009842:Oligopeptides / Q000737:chemistry*; D011956:Receptors, Cell Surface / Q000737:chemistry*
D009842:Oligopeptides; D011956:Receptors, Cell Surface; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D019791:Guanidine