[Simultaneous analysis of aspartic acid and sorbitol in potassium magnesium aspartate and sorbitol injection by ion-exclusion chromatography with evaporative light-scattering detection].
An ion-exclusion chromatographic (IEC) method with evaporative light-scattering detector (ELSD) was established for the simultaneous analysis of the aspartic acid and sorbitol in Potassium Magnesium Aspartate and Sorbitol Injection. This procedure eliminates the need for complex derivatization schemes since ELSD can detect aspartic acid and sorbitol directly. An ion exclusion column (TSKgel OApak-A column, 7.8 mm i.d. x 300 mm, 5 microns, Tosho, Japan) was used for separation. The mobile phase was a mixture of aqueous solution (containing 0.1% (volume fraction) trifluoroacetic acid)-methanol (9:1, volume ratio). Under the above conditions aspartic acid and sorbitol were separated to baseline in 12 min. Regression equations revealed linear relationship (correlation coefficient: 0.999) between the concentrations of the analytes injected and the peak areas of the analytes detected by ELSD. The detection limits of ELSD (S/N = 3) were about 30 mg/L and 20 mg/L for aspartic acid and sorbitol, respectively.
Wei
Yang
Y
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Ding
Ming-yu
MY
Zhang
Wen-qing
WQ
D001224:Aspartic Acid / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004338:Drug Combinations; D012542:Scattering, Radiation; D013012:Sorbitol / Q000032:analysis*
D004338:Drug Combinations; D001224:Aspartic Acid; D013012:Sorbitol