[Modulation and implications in pathogenesis of interferon gamma receptor 1 in patients with chronic hepatitis B].
OBJECTIVE
To study the possible differences in the interferon gamma receptor (IFN-gamma R1) response among a variety of clinical types in patients with chronic hepatitis B (CHB) and implications in pathogenesis.


METHODS
The serum level of IFN-gamma and the expression level of IFN-gamma R1 in peripheral leucocytes, from 53 CHB patients, were examined by ELISA and flow cytometry respectively, which were compared with the baseline levels of 15 healthy controls and were performed correlation analysis with alanine aminotransferase (ALT), total bilirubin (TBil) in serum and morphological change in hepatic tissues.


RESULTS
The results showed that the level of IFN-gamma R1 (28.89% 11.77%) expressed on the membranes of lymphocytes in CHB patients, which correlated with liver inflammation (r=0.621, P<0.01) and serum TBil level (r=0.575, P<0.01), was much higher than that (9.23% 1.30%) of the healthy controls (Z=3.988, P<0.05), and no obvious difference on the membranes of leucocytes. The serum levels of IFN-gamma in patients with cirrhosis and severe hepatitis were higher than those of healthy controls. And the two was no difference from each other, but the standard deviation in each group was relatively large.


CONCLUSIONS
These findings suggest that the IFN-gamma signal transduction pathway is modulated through up-regulating the expression of IFN-gamma R1 on the membranes of lymphocytes, which takes part in the immuno-pathogenesis in CHB patients.
Chen
Gong-ying
GY
Institute of Infectious Diseases, the First Affiliated Hospital Zhejiang University, Hangzhou 310003, China.
Chen
Zhi
Z
Li
Min-wei
MW
Liu
Hui-min
HM
Hu
Zhong-rong
ZR
D000328:Adult; D000368:Aged; D005260:Female; D019694:Hepatitis B, Chronic / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D008214:Lymphocytes / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D017471:Receptors, Interferon / Q000097:blood*; D000096743:Interferon gamma Receptor
D007371:Interferon-gamma; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor