Apoptosis in lymph nodes and changes in lymphocyte subpopulations in peripheral blood of pigs infected with porcine rubulavirus.
In a first experiment, five pigs were inoculated intranasally with porcine rubulavirus (PoRV) at 5 days of age and killed 7 days post-infection (pi). In a second experiment, four pigs were infected with the same virus at 17 days of age and killed at 9 or 15 days pi. Control piglets in each experiment received uninfected cell culture supernate. All PoRV-infected pigs developed respiratory and nervous signs, and histological lesions of non-suppurative encephalitis and interstitial pneumonia. All control pigs remained clinically normal and did not have histological lesions. Significantly increased numbers of apoptotic cells were detected by terminal deoxynucleotidyl transferase biotin-dUTP nick end labelling (TUNEL) in tonsil and lymph nodes of the pigs infected at 7 days of age and killed at 7 days pi. Significantly increased percentages of CD2(+) and CD8(+) T lymphocytes were also found in peripheral blood of these animals at this time, while the percentages of CD4(+) and MHC class II lymphocytes were significantly reduced. Significantly increased numbers of apoptotic cells were detected in lymphoid tissues of the pigs infected at 17 days of age and killed at 9 days pi. The percentages of CD2(+), CD8(+) and MHC class II lymphocytes in peripheral blood were also significantly increased at this time; the percentage of MHC class II lymphocytes remained elevated at 15 days pi. These results indicate that induction of apoptosis is an important mechanism in the pathogenesis of PoRV infection in young pigs, and that this virus induces changes in lymphocyte subpopulations in peripheral blood.
Rodríguez-Ropón
A
A
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas del IPN, Ciudad de México 11340, México.
Hernández-Jauregui
P
P
Sánchez-Torres
L
L
Favila-Castillo
L
L
Estrada-Parra
S
S
Moreno-López
J
J
Kennedy
S
S
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D020287:In Situ Nick-End Labeling; D008198:Lymph Nodes / Q000473:pathology* / Q000821:virology; D019213:Rubulavirus / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D019351:Rubulavirus Infections / Q000473:pathology / Q000503:physiopathology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000473:pathology* / Q000503:physiopathology / Q000821:virology; D016176:T-Lymphocyte Subsets / Q000473:pathology* / Q000821:virology