[Determination of purity of different types of aprotinin by micellar electrokinetic capillary chromatography].
A sensitive and rapid method was developed for purity determination of different types of aprotinin by micellar electrokinetic capillary chromatography (MECC). MECC was performed with 80 mmol/L Na2HPO4-H3PO4(pH 7.00) buffer solution containing 4 mmol/L cetyltrimethyl ammonium bromide (CTAB) at an applied voltage of 15 kV. The electrophoresis was monitored at 277 nm, and completed in 6 minutes. The relative standard deviations of migration time and area for aprotinin were below 0.3% and below 5% respectively within 1 day (for 6 repetitive runs). The effect of high concentration of salt in the samples has been studied. The results obtained by capillary zone electrophoresis, MECC and high performance liquid chromatography were compared, and those of MECC were the best.
Jiang
Ting-fu
TF
Lanzhou Institute of Chemical Physics, The Chinese Academy of Sciences, Lanzhou 730000, China.
Lu
Hao-jie
HJ
Li
Chen
C
Liang
Bing
B
Ou
Qing-yu
QY
D007611:Aprotinin / Q000032:analysis* / Q000302:isolation & purification; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods; D011208:Powders; D015842:Serine Proteinase Inhibitors / Q000032:analysis* / Q000302:isolation & purification
D011208:Powders; D015842:Serine Proteinase Inhibitors; D007611:Aprotinin