title: Arabidopsis mutants sensitive to gamma radiation include the homologue of the human repair gene ERCC1.
abstract: Mutants sensitive to ionizing radiation in yeast and mammals include an assortment of DNA repair genes. The majority of these DNA repair genes are involved in the repair of DNA double-strand breaks. In this study a forward genetic screen is used to identify gamma-sensitive mutants of Arabidopsis thaliana. The gamma-plantlet screen used here also reveals two general mutant classes based on size of cotyledons and hypocotyls. One of the mutants discovered is a homologue of the mammalian nucleotide excision repair gene ERCC1.
authors.affiliation: Department of Plant Biology, University of California-Davis, Davis, CA 95616, USA.
authors.forename: E
authors.initials: E
authors.lastname: Hefner
authors.forename: S B
authors.initials: SB
authors.lastname: Preuss
authors.forename: A B
authors.initials: AB
authors.lastname: Britt
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects*; D029681:Arabidopsis Proteins; D002453:Cell Cycle / Q000528:radiation effects; D018548:Cotyledon / Q000254:growth & development / Q000528:radiation effects; D004260:DNA Repair / Q000235:genetics* / Q000528:radiation effects; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins; D004307:Dose-Response Relationship, Radiation; D004720:Endonucleases; D005720:Gamma Rays; D005816:Genetic Complementation Test; D006801:Humans; D018546:Hypocotyl / Q000254:growth & development / Q000528:radiation effects; D018519:Meristem / Q000254:growth & development / Q000528:radiation effects; D009154:Mutation; D010641:Phenotype; D018517:Plant Roots / Q000254:growth & development / Q000528:radiation effects; D018520:Plant Shoots / Q000254:growth & development / Q000528:radiation effects; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D011506:Proteins; C071447:ERCC1 protein, human; D004720:Endonucleases; C499359:Ercc1 protein, Arabidopsis