[Chiral separation of thioglycidyl ether and glycidylselenide on cyclodextrin bonded stationary phase via high performance liquid chromatography].
beta- Cyclodextrin bonded chiral stationary phases (CD-CSP) were prepared after the method of Armstrong (CD-CSP I) and of Huang Tianbao (CD-CSP II). With the separation of o-, m- p-nitroaniline, it was found that the chromatographic properties of CD-CSP II was superior to that of CD-CSP I. Three racemes of thioglycidyl ethers and two racemes of glycidylselenides were resolved on CD-CSP II via high performance liquid chromatography. The mobile phase consisted of different volume ratios of methanol/0.3% triethylammonium acetate buffer solution(pH 5), and the eluate was detected with a UV-visible detector at a wavelength of 254 nm. With the increase of methanol volume ratio in the mobile phase, the retention time decreased rapidly, but within the range of methanol valume ratio 40/100 to 50/100, the separation factor only decreased slightly. The racemes examined contained aromatic groups and hydroxy groups which can form inclusion complexes and hydrogen bonding interactions with beta-cyclodextrin. The chiral separation of the racemes is successful with the mobile phase of V(methanol):V(buffer) = 40:60.
Yu
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Z
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000.
Zhou
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Z
Jiang
S
S
Chen
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L
D000814:Aniline Compounds / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003505:Cyclodextrins; D004852:Epoxy Compounds / Q000737:chemistry* / Q000302:isolation & purification; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D000814:Aniline Compounds; D003505:Cyclodextrins; D004852:Epoxy Compounds; D047392:beta-Cyclodextrins; C020344:glycidyl ethers; C008625:nitroaniline; C031215:betadex