title: Crystallization and preliminary X-ray diffraction studies of alpha-cyclodextrin glucanotransferase isolated from Bacillus macerans.
abstract: Single crystals of alpha-cyclodextrin glucanotransferase isolated from Bacillus macerans have been grown with polyethylene glycol 6000 as a precipitating agent by sitting-drop vapour diffusion at room temperature. The crystals were suitable for X-ray analysis and diffracted to at least 2.0 A (space group P2(1)2(1)2(1)), with unit-cell parameters a = 66.79 (2), b = 79.66 (1), c = 141.16 (1) A. Assuming the asymmetric cell to be occupied by a monomer of 74 kDa, the unit cell contains 42.6% solvent with a crystal Volume per protein mass, V(M), of 2.53 A(3) Da(-1).
authors.affiliation: Department of Chemistry, College of Natural Science, Chonbuk National University, 561-756 Chonju, South Korea. hwchoe@moak.chonbuk.ac.kr
authors.forename: Hui Woog
authors.initials: HW
authors.lastname: Choe
authors.forename: Kyo Sun
authors.initials: KS
authors.lastname: Park
authors.forename: Jörg
authors.initials: J
authors.lastname: Labahn
authors.forename: Joachim
authors.initials: J
authors.lastname: Granzin
authors.forename: Cheol Ju
authors.initials: CJ
authors.lastname: Kim
authors.forename: Georg
authors.initials: G
authors.lastname: Büldt
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D005964:Glucosyltransferases / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase