title: Full-length dystrophin cDNA transfer into skeletal muscle of adult mdx mice by electroporation.
abstract: We showed that a LacZ expression plasmid (pCAG-lacZ) injection followed by electroporation increased the expression of the LacZ gene in the skeletal muscles of adult mdx mice up to ninefold higher as compared with simple intramuscular DNA injection. When full-length mouse dystrophin plasmid (pCAG-dys) and pCAG-lacZ were co-transfected by electroporation, 56% of dystrophin-positive fibers were stained for beta-galactosidase activity suggesting most of these myofibers are not revertants but transfected ones. Our data indicate that electroporation in vivo could introduce large full-length dystrophin cDNA into skeletal muscle of adult mdx mice.
authors.affiliation: Division of Neurology, Department of Internal Medicine, Kawasaki Medical School, 577 Matsushima, Kurashiki City, Okayama 701-0192, Japan. tatsum@med.kawasaki-m.ac.jp
authors.forename: Tatsufumi
authors.initials: T
authors.lastname: Murakami
authors.forename: Toru
authors.initials: T
authors.lastname: Nishi
authors.forename: En
authors.initials: E
authors.lastname: Kimura
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Goto
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Maeda
authors.forename: Yukitaka
authors.initials: Y
authors.lastname: Ushio
authors.forename: Makoto
authors.initials: M
authors.lastname: Uchino
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Sunada
mesh_terms: D000367:Age Factors; D000818:Animals; D018076:DNA, Complementary / Q000493:pharmacokinetics; D016189:Dystrophin / Q000235:genetics*; D018274:Electroporation / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods*; D007763:Lac Operon; D051379:Mice; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000378:metabolism*; D009137:Muscular Dystrophy, Animal / Q000628:therapy*; D020388:Muscular Dystrophy, Duchenne / Q000628:therapy; D010957:Plasmids / Q000493:pharmacokinetics
chemical_list: D018076:DNA, Complementary; D016189:Dystrophin