title: [Preparation of immobilized metal-chelated affinity membrane and its application to purification of Cu/Zn-superoxide dismutase].
abstract: Immobilized metal-chelated affinity membranes were prepared using macropore cellulose filter paper as matrix. The matrix was treated with alkaline, activated with epichlorohydrin and coupled with iminodiacetate sodium, and then Cu2+ was immobilized. Cu/Zn-superoxide dismutase(SOD) was purified with prepared affinity membranes. The special activity of the purified Cu/Zn-SOD was increased 10.7 times from 645 U/mL to 6,882 U/mL. The protein recovery and the activity recovery were 92.3% and 985%, respectively. A new method to prevent the leaking of metal ion was developed and Cu2+ concentration in the effluent was lowered to 86 micrograms/L.
authors.affiliation: Department of Bioengineering, South China University of Technology, Guangzhou 510641, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Wei
authors.forename: R H
authors.initials: RH
authors.lastname: Yao
authors.forename: S X
authors.initials: SX
authors.lastname: Bao
mesh_terms: D002614:Chelating Agents; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods; D013482:Superoxide Dismutase / Q000302:isolation & purification*
chemical_list: D002614:Chelating Agents; D013482:Superoxide Dismutase