[The effect of autocrine factors on development of early embryos of mouse].
Two-cell-stage embryos were flushed from the oviducts on Day 2. Zygotes were collected from oviducts on Day 1 (Fertilization In Situ, ISF) or derived from fertilization in vitro (IVF). 2-cell embryos had a high rate of blastocyst development to each embryo concentration from 1 embryo/microliter to 1 embryo/1000 microliters. The zygotes produced by either ISF or IVF were adversely affected by reducing the embryo concentration over this range (P < 0.001), with approximately 82.5% of ISF zygotes developing to blastocysts at highest concentration but only 22.3% at the lowest. For IVF zygotes the corresponding results were 46.3% and 5.2%. The number of cells in each blastocyst from 2-cell embryos was significantly higher than that from ISF and IVF group. The media supplementing Platelet-activating factor (PAF) caused a significant increase in the rate of blastocyst development of IVF zygotes at embryo concentration of 1 embryo/10 microliters (10 ng/ml) and 1 embryo/100 microliters (100 ng/ml). Insulin-like growth factor (IGF) (10 ng/ml) also stimulated development of IVF zygotes when they were cultured at the concentration of 1 embryo/10 microliters. Epidermal growth factor (EGF) was no effect over range of 1-1000 ng/ml to embryo development. The results show that factors necessary for normal embryo development are diluted to suboptimal levels during culture at low embryo concentration. The PAF, IGF-I partially compensate the effects of low embryo concentration during culture and play important roles as autocrine embryotrophic factors.
Jin
Y
Y
Institute of Laser & Life Science, South China Normal University, Guangzhou 510631.
Guo
X Z
XZ
Li
L
L
Xie
C Y
CY
Tan
L L
LL
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology; D046508:Culture Techniques; D005314:Embryonic and Fetal Development / Q000187:drug effects / Q000502:physiology*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D051379:Mice; D010972:Platelet Activating Factor / Q000494:pharmacology*; D015053:Zygote / Q000187:drug effects
D010972:Platelet Activating Factor; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I