[Cloning and sequence analysis of E0 gene of hog cholera virus lapinized Chinese strain and virulent shimen strain].
According to the published nucleotide sequences of genome of hog cholera virus, one pair of specific primers were designed and synthesized. From the spleen of rabbits which were infected with HCLV and HCV Shimen strain infected pig blood, the two E0 genes were amplified by RT-PCR. The amplified fragments were cloned into pGEM-T vector and sequenced. Sequence analysis showed nucleotide sequence and deduced amino acid sequence homologies of the E0 gene between HCLV and Shimen strain were 95.0% and 94.3%. There was 13 amino acid substitutions between them. One N-glycosylation site was missing from E0 gene of the Shimen strain. The nucleotide sequence homologies of the Shimen strain with the ALD, GPE-, Brescia and Alfort strains were 97.4%, 96.5%, 92.2% and 86.5%, respectively, the deduced amino acid sequence homologies were 97.4%, 96.0%, 95.2% and 92.5%, respectively. The nucleotide sequence homologies of E0 gene of the HCLV strain with the ALD, GPE-, Brescia and Alfort strains were 95.6%, 94.9%, 91.3% and 85.5%, respectively, the deduced amino acid sequence homologies were 93.4%, 92.5%, 91.6% and 90.7%, respectively. E0 gene from HCV was shown to be similar to a family of fungal and plant ribonuclease. The catalytically important residues were 28 to 40 and 71 to 89 of HCV E0 gene.
Wang
J
J
Institute of Virology, Wuhan University, Wuhan 430072.
Zhang
C
C
Wang
N
N
Fu
L
L
Huang
Q
Q
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D006692:Classical Swine Fever Virus / Q000235:genetics*; D003001:Cloning, Molecular; D005814:Genes, Viral; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013154:Spleen / Q000821:virology; D013552:Swine; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D006023:Glycoproteins; D014764:Viral Proteins