[Effect of the bonding amounts of chiral ligand exchange chromatographic stationary phases on the enantioseparation of amino acids].
L-proline chiral stationary phases with various bonding amounts for ligand exchange chromatography were prepared by treating silica gel with 3-glycidoxypropyltrimethoxysilane and bonding L-proline. The bonding amounts of L-proline chiral stationary phase were controlled effectively and characterized by IR and elemental analysis. Some DL-amino acids were separated and the influences of the bonding amounts on the efficiency of resolution of DL-amino acids by ligand exchange chromatography were briefly discussed and explained by the mechanism of the separation.
Zhu
Xin-yi
XY
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China. zxylz@yahoo.com
Han
Xiao-qian
XQ
Qi
Bang-feng
BF
Li
Yong-min
YM
Jiang
Sheng-xiang
SX
Chen
Li-ren
LR
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D002942:Circular Dichroism; D006860:Hydrogen Bonding; D008024:Ligands; D011392:Proline / Q000302:isolation & purification; D013055:Spectrophotometry, Infrared; D013237:Stereoisomerism; D014443:Tyrosine / Q000302:isolation & purification
D000596:Amino Acids; D008024:Ligands; D014443:Tyrosine; D011392:Proline