title: Molecular biological characterization of equine surfactant protein A.
abstract: In the following, we describe the isolation and sequencing of the equine surfactant protein A (Sp-A) as found in both the cDNA and the genomic DNA. We found a length of the cDNA sequence of 747 bp (base pairs), in translation into amino acids of 248. Compared with the known molecular biological facts about Sp-A in other species, the cDNA sequence obtained showed highest homology with that of sheep (85.01%). The genomic DNA of equine Sp-A, as in other species, includes three introns. There were no hints for the existence of two different Sp-A genes. These results should form the basis for a better understanding of respiratory failure in foals and adult horses, and also lead to further studies on this item.
authors.affiliation: Interdisciplinary Research Team on Perinatology/Neonatology Justus-Liebig-University, Giessen, Germany. rainer.hospes@vetmed.uni-giessen.de
authors.forename: R
authors.initials: R
authors.lastname: Hospes
authors.forename: B I L
authors.initials: BI
authors.lastname: Hospes
authors.forename: I
authors.initials: I
authors.lastname: Reiss
authors.forename: H
authors.initials: H
authors.lastname: Bostedt
authors.forename: L
authors.initials: L
authors.lastname: Gortner
mesh_terms: D000818:Animals; D017931:DNA Primers; D006734:Horse Diseases / Q000235:genetics; D006736:Horses / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics*; D012131:Respiratory Insufficiency / Q000235:genetics / Q000662:veterinary
chemical_list: D017931:DNA Primers; D037662:Pulmonary Surfactant-Associated Protein A