[Analysis of rRNA gene restriction fragments length polymorphism of Leptospira in China].
Sixty-four Leptospira international and domestic reference strains, which belonging to fifty-four serovars, and twenty-seven field strains were examined by using EcoR I restriction endonuclease analysis of genomic DNA and restriction fragments length polymorphism of rRNA gene, fifty-six Leptospiral ribotypes(RTs) were described. Most serovars gave specific patterns. Serovars in the same serogroup possess common core-segments, but we found RTs of reference strains from China and other countries are different. Most field strains have RTs with correspond of reference strains, only a few bands were shown different if RTs were different. A notable result was that the field strains of serovar pomona have the same Rt as the international reference strain but different from the domestic reference strain.
Feng
X
X
Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206.
Qin
J
J
Liang
Z
Z
Shi
M
M
Wang
Y
Y
Nie
Y
Y
D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D007919:Leptospira / Q000145:classification* / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics*; D015183:Restriction Mapping; D012703:Serotyping
D004269:DNA, Bacterial; D012335:RNA, Ribosomal