[Enhancement of BCG-induced hBD-1 mRNA expression in human pulmonary gland epithelial cells].
Using reverse-transcription PCR (RT-PCR) and Northern blot analysis, the present authors found an enhanced expression of the human beta-defensin 1 (hBD-1) gene in the SPC-A-1 cells challenged with heat-killed M. Bovis Bacille Calmette-Guerin (BCG). The enhanced expression of hBD-1 mRNA was dose- and time-dependent. This result indicated a significant progress in the work of developing a new antimicrobial strategy that enhances mucosa antibiotic peptide expression for the prevention and treatment of mucosal infections.
Feng
Y
Y
Research Unit of Infection & Immunity, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
Huang
N
N
Wu
Q
Q
Wang
B
B
D001500:BCG Vaccine / Q000494:pharmacology*; D001483:Base Sequence; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000166:cytology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D023083:beta-Defensins / Q000096:biosynthesis* / Q000235:genetics
D001500:BCG Vaccine; C406407:DEFB1 protein, human; D012333:RNA, Messenger; D023083:beta-Defensins