Blood extracellular superoxide dismutase levels in hemodialysis patients pre- and post-hemodialysis and its association with lipoprotein lipase mass and free fatty acid.
BACKGROUND
Hemodialysis (HD) is a method of chronic therapy for patients with renal failure. Diabetic nephropathy is the most common underlying disease among HD patients in Japan. A characteristic problem associated with this condition is endothelial cell damage. We have been using extracellular superoxide dismutase (EC-SOD) attached to the heparan sulfate on the endothelial cell surface as a marker of vascular damage.


METHODS
This study examined the pre- and post-HD levels of lipoprotein lipase (LPL). The increase in free fatty acid (FFA) is affected by a type of cytokine-like substances, which induces insulin resistance and causes abnormal lipid metabolism.


RESULTS
Pre-HD blood samples showed highly significant correlations between EC-SOD and LPL mass (r = 0.792) and between EC-SOD and FFA (r = 0.675) (p < 0.0001). EC-SOD, LPL mass, and FFA were remarkably high among the patients who had been placed on HD treatment for over 20 years.


CONCLUSIONS
Because EC-SOD and LPL mass represent heparin-binding proteins, these results were considered to reflect severe vascular damage.
Shimomura
Hiroji
H
Central Clinical Laboratories, Mitsui Memorial Hospital, 1 Kanda Izumi-cho, Chiyoda-ku, Tokyo 101-8643, Japan. hshimo@mitsuihosp.or.jp
Maehata
Eisuke
E
Takamiya
Tomi
T
Adachi
Tetsuo
T
Komoda
Tsugikazu
T
D000328:Adult; D000368:Aged; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins / Q000097:blood; D004730:Endothelium, Vascular / Q000473:pathology; D005110:Extracellular Space / Q000201:enzymology*; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005260:Female; D006801:Humans; D008071:Lipoprotein Lipase / Q000097:blood*; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis / Q000009:adverse effects*; D013482:Superoxide Dismutase / Q000097:blood*
C062991:AZU1 protein, human; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins; D005230:Fatty Acids, Nonesterified; D013482:Superoxide Dismutase; D008071:Lipoprotein Lipase