[Internalization and replication of Edwardsiella tarda in HEp-2 cells].
It is demonstrated that Edwardsiella tarda possesses the ability to invade cultured epithelial HEp-2 cells by both lysis-counting assay and thin-section electron microscopy. Among fifteen strains of E. tarda, 6 strains internalized into HEp-2 cells and located mainly in vesicles. After internalization, the bacteria replicated in the host cells and then released into medium. Pretreatment of the HEp-2 cells with various concentration of cytochalasin D (0.1-5.0 micrograms/ml) or cytochalasin B (2.5-10.0 micrograms/ml) significantly reduced the amount of internalized bacteria in a dose-dependent manner. While pretreatment of HEp-2 cells with 0.25-100 mumol/L colchicine did not show any effect on the invasiveness of E. tarda. This strongly indicates that microfilaments are required for the internalization of E. tarda into HEp-2 cells and that microtubules are not involved in the entrance of E. tarda into HEp-2 cells.
Ma
X
X
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095.
Ouyang
Z
Z
Chen
H
H
Lu
C
C
Xia
X
X
D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000382:microbiology / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D020609:Edwardsiella tarda / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology*; D004848:Epithelium / Q000382:microbiology; D006801:Humans; D014774:Virulence
D015638:Cytochalasin D; D003571:Cytochalasin B; D003078:Colchicine