[Determination of catechol-O-methyltransferase activity in human erythrocytes by high performance liquid chromatography].
A rapid assay method for catechol-O-methyltransferase (COMT) activity in human erythrocyts by high performance liquid chromatography (HPLC) with UV detection was established. Enzyme activity was determined from erythrocyte lysates using S-adenosyl-L-methionine (SAM) as methyl donor and 3, 4-dihydroxybenzoic acid (DBA) as substrate. The 3-O-methylated reaction products were measured by HPLC with UV detection. The linear range of COMT was from 1 U/mL to 60 U/mL with an average RSD < 10%, and the detection limit was 0.5 U/mL(S/N > or = 5).
Yan
X H
XH
Shenzhen Institute of Mental Health, Shenzhen 518003, China.
Deng
X M
XM
Wu
H A
HA
D000293:Adolescent; D000328:Adult; D002394:Catechol O-Methyltransferase / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D003863:Depression / Q000201:enzymology; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006801:Humans; D008297:Male
D002394:Catechol O-Methyltransferase