[Expression and implication of c-myb and bcl-2 proteins in C6 gliomas].
This study was aimed at the level and implication of expression of c-myb and bcl-2 proteins in C6 glioma. The therapeutic effect of oncogene c-myb antisense oligodeoxynucleotide on C6 glioma in nude mice was studied. C6 glioma cells were implanted into the cutis of 36 nude mice. Ten days later, the nude mice were randomly divided into three groups and were treated with SON, AON and normal saline respectively. Three mice of every group were killed at 4 days, 8 days, 12 days and 16 days after treatment, respectively. The levels of c-myb and Bcl-2 proteins expression in the three groups were observed by the S-P immunohistochemical method. The results showed the expression of c-myb and bcl-2 proteins was significantly decreased in the AON group, compared with that in the SON and control groups (P < 0.05). These data suggest that c-myb and bcl-2 proteins may play an important role in malignant glioma, and c-myb gene and bcl-2 gene may be involved in the tumorigenesis and development of glioma.
You
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Department of Neurosurgery, First Affiliated Hospital, WCUMS, Chengdu 610041.
Xu
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X
Cal
B
B
Zhang
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S
D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005910:Glioma / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D020319:Oligodeoxyribonucleotides, Antisense / Q000627:therapeutic use; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D020598:Proto-Oncogene Proteins c-myb / Q000096:biosynthesis* / Q000235:genetics; D011897:Random Allocation
D020319:Oligodeoxyribonucleotides, Antisense; D019253:Proto-Oncogene Proteins c-bcl-2; D020598:Proto-Oncogene Proteins c-myb