title: Reciprocal action between BMP-2 and BMP-3 in cultured fibroblast in vitro.
abstract: OBJECTIVE
  To explore reciprocal action between BMP-2 (bone morphogenetic protein-2) and BMP-3 for better understanding of the mechanism of BMP during bone fracture union.
  
  
  METHODS
  rhBMP-2 was added into the cultured fibroblasts with the concentration of 1,200 ng/ml. The expression of BMP-3 in fibroblasts was detected by immunohistochemistry. Eukaryotic expression vector pcDNA3-BMP-3 was transfected into the fibroblasts. After the effective expression of BMP-3 was identified, BMP-2 was also detected by immunohistochemistry in BMP-3 expression cells. The fibroblasts transfected with empty vector pcDNA3 were used as the control.
  
  
  RESULTS
  Exogenous rhBMP-2 could promote the expression of BMP-3 in fibroblasts. BMP-3 also could be detected in these cells.
  
  
  CONCLUSIONS
  BMP-2 and BMP-3 could reciprocally adjust the expression in fibroblasts.
authors.affiliation: Orthopaedic Institute of PLA, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. mengguolin@163.net
authors.forename: Guo-lin
authors.initials: GL
authors.lastname: Meng
authors.forename: Yun-yu
authors.initials: YY
authors.lastname: Hu
authors.forename: Qin
authors.initials: Q
authors.lastname: Pu
authors.forename: Rong
authors.initials: R
authors.lastname: Lu
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Bai
authors.forename: Xin-zhi
authors.initials: XZ
authors.lastname: Xu
authors.forename: Dan
authors.initials: D
authors.lastname: Li
mesh_terms: D055396:Bone Morphogenetic Protein 2; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D017102:Fracture Healing / Q000502:physiology*; D007150:Immunohistochemistry; D010012:Osteogenesis / Q000502:physiology*; D016212:Transforming Growth Factor beta
chemical_list: D055396:Bone Morphogenetic Protein 2; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins; D016212:Transforming Growth Factor beta