title: Induction of hemopoiesis by saenghyuldan, a mixture of Ginseng radix, Paeoniae radix alba, and Hominis placenta extracts.
abstract: OBJECTIVE
  To examine the efficacy of saenghyuldan and its components, Ginseng Radix, Paeoniae Radix Alba, and Hominis Placenta extracts (SHD, GR, PRA, and HP, respectively) on the hemopoiesis in a myelosuppression model system.
  
  
  METHODS
  Susceptibility to cyclophosphamide (CP) and S180 carcinoma was determined in SHD, GR, PRA, and HP-treated mice. Analysis of peripheral blood and bone marrow cells was demonstrated by changes in cell types and histopathologic examination. The expression of cytokine mRNAs involved in hemopoiesis was examined by RT-PCR.
  
  
  RESULTS
  SHD and its separated components (GR, HP, and PRA, respectively) significantly increased the survival in CP- and S180-treated mice. The hematology data demonstrated that all the agents augmented monocyte and leucocyte counts in the peripheral blood and increased bone marrow density and the ratio of leukocyte to erythrocyte in the bone marrow. These findings were positively correlated with the up-regulation of cytokine mRNA expression such as granulocyte colony-stimulating factor (GM-CSF), erythropoietin (EPO), thrombopoietin (TPO), stem cell factor (SCF), and c-Kit.
  
  
  CONCLUSIONS
  SHD is an effective remedy for the bone marrow failure and myelosuppression occurring during chemotherapy.
authors.affiliation: Department of Internal Medicine, College of Oriental Medicine, Daejeon University, 301-724, Korea. ckson@dju.ac.kr
authors.forename: Chang-Gue
authors.initials: CG
authors.lastname: Son
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Han
authors.forename: Jung-Hyo
authors.initials: JH
authors.lastname: Cho
authors.forename: Jang-Woo
authors.initials: JW
authors.lastname: Shin
authors.forename: Chin-Ho
authors.initials: CH
authors.lastname: Cho
authors.forename: Yeon-Weol
authors.initials: YW
authors.lastname: Lee
authors.forename: Chong-Kwan
authors.initials: CK
authors.lastname: Cho
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004338:Drug Combinations; D004921:Erythropoietin / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000235:genetics; D006410:Hematopoiesis / Q000187:drug effects*; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D009368:Neoplasm Transplantation; D029596:Paeonia / Q000737:chemistry; D005894:Panax / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012510:Sarcoma 180 / Q000188:drug therapy / Q000473:pathology; D019089:Stem Cell Factor / Q000096:biosynthesis / Q000235:genetics; D013926:Thrombopoietin / Q000096:biosynthesis / Q000235:genetics
chemical_list: D016207:Cytokines; D004338:Drug Combinations; D008419:Materia Medica; D010936:Plant Extracts; D012333:RNA, Messenger; D019089:Stem Cell Factor; D004921:Erythropoietin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D013926:Thrombopoietin