[Design and synthesizing of human vascular endothelial growth factor (VEGF) peptide].
OBJECTIVE
To predict continuous epitopes of VEGF using synthetic peptide of VEGF as antigen to produce anti-VEGF-peptide monoclonal antibody.


METHODS
According to amino sequence of VEGF189, antigen index program and Goldkey soft were used to predict VEGF epitopes, and the peptide containing one of predicted epitopes was synthesized by 430A Peptide Synthesizer.


RESULTS
The NH2-terminal 1-26 amino acid sequence of VEGF was determined as synthetic peptide and synthesized.


CONCLUSIONS
The predicted epitope of VEGF was synthesized, which provided an effective method when lacking of VEGF.
Yang
X
X
Department of Oral and Maxillofacial Surgery, College of Stomatology, West China University of Medical Sciences.
Wang
D
D
Zheng
G
G
Fang
S
S
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D016228:Endothelial Growth Factors / Q000737:chemistry*; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry*; D008222:Lymphokines / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D000911:Antibodies, Monoclonal; D016228:Endothelial Growth Factors; D000939:Epitopes; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D010455:Peptides; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors