[Nitric oxide mediates the inhibitory effects of interleukin-2 on the isolated ventricular papillary muscle and cultured neonatal rat cardiomyocyte]
OBJECTIVE: To explore the effects and mechanism of interleukin-2 on the isolated rat ventricular papillary muscle and cultured cardiomyocyte. METHODS: The isolated rat papillary muscle and cultured neonatal rat cardiomyocyte were used to determine the effects on contractile properties and spontaneous beating. RESULTS: IL-2 inhibited the contractile force of isolated rat papillary muscle and reduced the spontaneous beating rate of cultured neonatal rat cardiomyocytes in a dose-dependent manner. IL-2 at high concentration reduced the systolic duration and prolonged the diastolic duration. Pretreatment with L-NAME, an inhibitor of nitric oxide synthase,abolished the effects of IL-2. Heat inactivated IL-2 had no effect on papillary muscles and cardiomyocytes.CONCLUSION: Inhibitory effects of IL-2 on the contraction of isolated papillary muscle and spontaneous beating rate of cultured neonatal cardiomyocyte are mediated by nitric oxide.
Wang
Lin-Lin
LL
College of Medical Sciences, Zhejiang University, Hangzhou 310031, China.
Lin
Guo-Hua
GH
Chen
Ying-Ying
YY
Shen
Yue-Liang
YL
Xia
Qiang
Q