Seroreactivity of purified Brugia malayi microfilarial soluble and excretory-secretory antigens in different clinical presentations of bancroftian filariasis.
Brugia malayi microfilarial excretory-secretory (mf ES) and phosphate buffer saline soluble (mf S) antigens were fractionated by fast protein liquid chromatography (FPLC) on superdex 200 HR 10/30 gel filtration column. The active antigen fractions were identified and explored in comparison with whole mf ES and mf S antigens to detect filarial IgG antibodies in different groups viz microfilaraemics, acute, chronic and occult filarial cases of Wuchereria bancrofti infection and endemic and non-endemic normals. One of the fractions of mf ES antigen (ESF-6) and two fractions of mf S antigen (SF-2 & 3) were identified to be useful to detect filarial antibodies. A pooled preparation of these antigen fractions gave a sensitivity of 86.6% (for microfilaraemic cases) and a specificity of 95% to detect filarial IgG antibodies by indirect ELISA. The pooled FPLC purified mf antigens also showed 55-88% of cases of different grades of clinical filariasis and 65% of tropical pulmonary eosinophilia cases as positive for filarial antibodies. The pooled FPLC purified B. malayi mf antigens with higher specificity are preferable to whole mf ES and mf S antigens to detect active filarial infection in microfilaraemia and as well in different clinical entities of bancroftian filariasis.
Alli
R
R
Department of Biochemistry & JB Tropical Disease Research Centre, MGIMS, Sevagram (Wardha) 442 102, India.
Bhandari
Y P
YP
Reddy
M V
MV
Harinath
B C
BC
D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D017178:Brugia malayi / Q000276:immunology*; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D005368:Filariasis / Q000097:blood* / Q000276:immunology; D012680:Sensitivity and Specificity
D000947:Antigens, Helminth