[Cloning and expression of Vitreoscilla hemoglobin in Streptomyces aureofaciens].
Vitreoscilla hemoglobin Gene was cloned in Streptomyces aureofaciens through E. coli-Streptomyces shuttle plasmids constructed by both pIJ699-pUC19(vhb) and pIJ702-pBR322(vhb). Under low dissolved oxygen conditions, expression of hemoglobin enhanced CTC yield of engineering strain more about 40%-60% that that of control by improving oxygen transmission in cells. Hemoglobin expression by Promoter of tetracycline resistance gene was more effective for oxygen transmission than that by its native promoter regulated by dissolved oxygen concentrations in environments of locally low concentrations of dissolved oxygen.
Meng
Chun
C
Department of Food Science and Biotechnology, Fuzhou University, Fujian 350002, China.
Ye
Qin
Q
Shi
Xian'ai
X
Qiu
Li
L
Song
Siyang
S
Guo
Yanghao
Y
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D002751:Chlortetracycline / Q000096:biosynthesis*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006454:Hemoglobins / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013304:Streptomyces aureofaciens / Q000235:genetics* / Q000378:metabolism; D013753:Tetracycline Resistance / Q000235:genetics; D054793:Truncated Hemoglobins
D001426:Bacterial Proteins; D006454:Hemoglobins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D002751:Chlortetracycline