title: Effects of zinc occupancy on human O6-alkylguanine-DNA alkyltransferase.
abstract: A recent crystallographic study of recombinant human O(6)-alkylguanine-DNA alkyltransferase (hAGT) revealed a previously unknown zinc atom [Daniels et al., (2000) EMBO J. 19, 1719-1730]. The effects of zinc on the properties of hAGT are reported here. In bacterial expression systems, recombinant hAGT was produced in increasingly larger quantities when growth media are supplemented with up to 0.1 mM ZnCl(2). Metal-enriched hAGT samples had a 5-fold increase in repair rate constant over conventionally purified protein samples and a 60-fold increase over metal-stripped hAGT. In addition, mutants of the zinc-binding residues had decreases in zinc occupancy that correlated with reductions in repair rate. Zinc modulation did not abolish the repair capacity of a fraction of the hAGT population, as evidenced by the stoichiometric reaction with an oligodeoxyribonucleotide substrate. Zinc occupancy had a similar effect on the rate of reaction with O(6)-benzylguanine, a free base substrate, as on the repair of methylated DNA. Differentially zinc-treated hAGTs showed the same affinity for binding to native DNA and substrate oligodeoxyribonucleotides. Metal content manipulations had little effect upon the CD spectrum of hAGT, but fluorescence studies revealed a small conformational change based upon metal binding, and zinc occupancy correlated with enhanced hAGT stability as evidenced by resistance to the denaturing effects of urea. These results indicate that the presence of zinc confers a mechanistic enhancement to repair activity that does not result from an increase in substrate binding affinity. Zinc also provides conformational stability to hAGT that may influence its regulation.
authors.affiliation: Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Rasimas
authors.forename: Sreenivas
authors.initials: S
authors.lastname: Kanugula
authors.forename: Paula M
authors.initials: PM
authors.lastname: Dalessio
authors.forename: Ira J
authors.initials: IJ
authors.lastname: Ropson
authors.forename: Michael G
authors.initials: MG
authors.lastname: Fried
authors.forename: Anthony E
authors.initials: AE
authors.lastname: Pegg
mesh_terms: D001665:Binding Sites; D002712:Chlorides / Q000737:chemistry; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000737:chemistry; D004795:Enzyme Stability; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008667:Metalloproteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000737:chemistry; D016297:Mutagenesis, Site-Directed; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000737:chemistry*; D010957:Plasmids; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D015032:Zinc / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D002712:Chlorides; D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors; D008667:Metalloproteins; D011994:Recombinant Proteins; D017967:Zinc Compounds; C064976:O(6)-benzylguanine; D006147:Guanine; C016837:zinc chloride; D019853:O(6)-Methylguanine-DNA Methyltransferase; D015032:Zinc