title: High-volume, zero balanced ultrafiltration improves pulmonary function in a model of post-pump syndrome.
abstract: The systemic inflammatory response syndrome (SIRS), which may develop following cardiopulmonary bypass (CPB), can cause postoperative complications that contribute to the morbidity and mortality associated with open-heart surgery. Inflammatory mediators such as cytokines, are thought to play an important role in SIRS. Zero Balance Ultrafiltration (Z-BUF) is thought to reduce the quantity of inflammatory mediators associated with CPB and may attenuate the adverse effects of bypass. Following ethics committee approval, both an unfiltered experimental group and Z-BUF treatment group consisting of Yorkshire pigs (41 +/- 19 kg) were anesthetized, ventilated, instrumented, cannulated and placed on CPB for 60 minutes. Following CPB, an infusion of low-dose endotoxin (1 microg/kg) was administered I.V. and the animals were monitored for 3.5 hours. The Z-BUF treatment group (n = 5) received high-volume Z-BUF (122 ml/kg +/- 41) and the unfiltered experimental group (n = 5) did not. Hemodynamics, blood gases, and pulmonary functions were measured before, during, and after CPB. Following euthanasia, the middle lobe of the lung was prepared for histological analysis. Necropsy of the lung sample was weighed before and after dehydration to evaluate lung water content. During the experimental time course, plasma samples were evaluated for Interleukin-8 (IL-8) concentrations. Arterial PO2's (mmHg) in the unfiltered experimental group showed a significant reduction at 3.5 hours post CPB when compared to baseline while the Z-BUF treatment group PaO2 did not significantly change. There was a significant difference in the PaO2 between the unfiltered experimental and Z-BUF group at the final 3.5 hour time point (78 +/- 32 vs. 188 +/- 92 mmHg respectively). Pulmonary compliance (ml/cmH2O) was significantly reduced in both the unfiltered experimental and Z-BUF treatment groups with the unfiltered experimental group being the most significant. Lung wet/dry ratios were established and results found the unfiltered experimental group ratio significantly greater than that of the Z-BUF treatment group. Morphometric analysis of histologic lung sections confirmed pulmonary injury in the unfiltered experimental group and protection in the Z-BUF treatment group. This study suggests that Z-BUF provides higher arterial PO2's and lung compliances while reducing pulmonary edema and lung injury in a porcine model of PPS.
authors.affiliation: Department of Cardiovascular Perfusion, Upstate Medical University, Syracuse, New York 13210, USA.
authors.forename: Edward
authors.initials: E
authors.lastname: Darling
authors.forename: Bruce
authors.initials: B
authors.lastname: Searles
authors.forename: Fadi
authors.initials: F
authors.lastname: Nasrallah
authors.forename: Michael
authors.initials: M
authors.lastname: Robins
authors.forename: Xiaomang
authors.initials: X
authors.lastname: You
authors.forename: Louis
authors.initials: L
authors.lastname: Gatto
authors.forename: Nicole
authors.initials: N
authors.lastname: Clay
authors.forename: Anthony
authors.initials: A
authors.lastname: Picone
authors.forename: Jay
authors.initials: J
authors.lastname: Steinberg
authors.forename: Gary
authors.initials: G
authors.lastname: Nieman
mesh_terms: D000818:Animals; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D004573:Electrolytes / Q000097:blood; D015633:Extravascular Lung Water; D006439:Hemodynamics; D016209:Interleukin-8 / Q000097:blood; D023421:Models, Animal; D012128:Respiratory Distress Syndrome / Q000503:physiopathology / Q000628:therapy*; D012129:Respiratory Function Tests; D013552:Swine; D014462:Ultrafiltration / Q000295:instrumentation*; D014481:United States
chemical_list: D004573:Electrolytes; D016209:Interleukin-8