Use of polymerase chain reaction to detect the soft rot pathogen, Pythium myriotylum, in infected ginger rhizomes.
OBJECTIVE
The aims are to establish a polymerase chain reaction (PCR)-based method for detecting Pythium myriotylum in the rhizome of ginger and diagnosing ginger soft rot and screening health seed ginger.


RESULTS
A booster PCR method was established for detection of P. myriotylum using a specific primer selected from rDNA ITS1 region coupled with universal primer ITS2. It successfully applied to the detection of P. myriotylum in naturally infected ginger rhizomes but not from DNA of ginger rhizomes collected from field without target fungus.


CONCLUSIONS
A specific method for detecting P. myriotylum was achieved.


CONCLUSIONS
The new PCR method has allowed us to monitor ginger for the presence of P. myriotylum as a way of disease diagnosis or healthy seed ginger examination.
Wang
P H
PH
Department of Microbiology, Soochow University, Taipei 11102, Taiwan, ROC. phwang@mail.scu.edu.tw
Chung
C Y
CY
Lin
Y S
YS
Yeh
Y
Y
D020939:Zingiber officinale / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011775:Pythium / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D013624:Taiwan; D019914:Taq Polymerase
D019914:Taq Polymerase