[Expression of thermostable alpha-amylase gene in potato].
The thermostable alpha-amylase gene cloned from Bacillus lichemiformis was reconstructed into an expression vector pAMY721M under the CaMV35S promoter. The vector was transferred into A. tumerfaciens ABI. The thermostable alpha-amylase gene was transferred into Solanum tuberosum L. via Agrobacterium mediation according to the revised method of ZHAO Shu Juan et al (1997) and YANG Mei Zhu et al (1992). Shoots were induced and regenerated on MS medium with 2 mg/L ZT, 0.1 mg/L IAA and 100 mg/L kanamycin. Putative transformants were selected with kanamycin and roots induced on MS medium with 0.15 mg/L IAA. PCR analysis and thermostable alpha-amylase activity assay were done to identify the transgenic plantlets. Among them, 102,001, 102,607, and 110,402 were showed to have a higher alpha-amylase activity than untransformed control.
Zhao
S J
SJ
Department of Biology of East China Normal University, Shanghai 200062.
Huang
X H
XH
Wang
S P
SP
D016960:Agrobacterium tumefaciens / Q000201:enzymology; D001407:Bacillus / Q000201:enzymology; D015870:Gene Expression; D005822:Genetic Vectors; D030821:Plants, Genetically Modified; D011198:Solanum tuberosum / Q000235:genetics*; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
D000516:alpha-Amylases