title: Effect of an anti-androgen on testicular descent and inguinal closure in a marsupial, the tammar wallaby (Macropus eugenii).
abstract: Androgens are essential for testicular descent in eutherian mammals, but little is known about its hormonal control in marsupials. This study reports the effects of daily treatment with the anti-androgen flutamide (10 mg kg(-1)) from day 9 to day 75 after birth on the descent of the testis and inguinal closure in tammar wallabies. By day 75 after birth, the testes of control males had descended and the prostate gland was well developed. The testes of all flutamide-treated males had passed through the inguinal canal and were situated in the base of the scrotum. Three of the nine flutamide-treated males had unilateral inguinal hernias. The size of the inguinal canal, regardless of whether a hernia was present, was significantly wider than that of control males. Development of the prostate gland was significantly inhibited. By day 75 after birth, the phallus was significantly longer in control males than in females, whereas the phallus of flutamide-treated males was similar to that of control females. In flutamide-treated males, the lumbar 1 dorsal root ganglia was feminized and significantly fewer cell bodies expressed calcitonin gene- related peptide. As the anti-androgen treatment resulted in a reduction in the number of calcitonin gene-related peptide-positive cell bodies in the dorsal root ganglion supplying the genitofemoral nerve, the process of inguinal closure in tammar wallabies may be mediated by calcitonin gene-related peptide via the genitofemoral nerve, as indicated in humans. Flutamide treatment inhibited development of the prostate gland and phallus, which are both androgen-dependent structures, but it did not affect the normal descent of the testis, indicating that testicular descent can proceed when the action of androgens is blocked.
authors.affiliation: Department of Zoology, University of Melbourne, Victoria 3010, Australia. d.coveney@cellbiol.duke.edu
authors.forename: D
authors.initials: D
authors.lastname: Coveney
authors.forename: G
authors.initials: G
authors.lastname: Shaw
authors.forename: J M
authors.initials: JM
authors.lastname: Hutson
authors.forename: M B
authors.initials: MB
authors.lastname: Renfree
mesh_terms: D000726:Androgen Antagonists / Q000494:pharmacology*; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D005485:Flutamide / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000737:chemistry / Q000187:drug effects; D007264:Inguinal Canal / Q000254:growth & development*; D007633:Keratins / Q000032:analysis; D007614:Macropodidae; D008297:Male; D008856:Microscopy, Fluorescence; D010413:Penis / Q000187:drug effects; D011467:Prostate / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000254:growth & development*
chemical_list: D000726:Androgen Antagonists; D007633:Keratins; D005485:Flutamide; D015740:Calcitonin Gene-Related Peptide