[Classification for two isolates of Microbispora producing immunosuppressants].
Two strains named SIPI-226 and SIPI-207 producing immunosuppressants were isolated from soil samples collected in park of Wuxi city in China. Identification was done by morphological observation, chemical analysis and nucleic acid ribotyping and 16S rRNA sequence. Two strains contain meso-diaminopimelic acid in cell walls, Type PIV phospholipids, a lack of mycolic acids, MK-9(H0, H2, H4) menaquinones and G + C content of 68.3% to 69.4%, and two strains have 97.2% to 98.6% homology in 16S rRNA gene sequences, and the DNA homology by Ribotyping are 53% to 70%, but differ from seven other mesophilic Microbispora rosea strains. It could be concluded that these two strains represented two new subspecies in the genus of Microbispora rosea. We designated the strains as Microbispora rosea subsp. wuxiensis and Microbispora rosea subsp. yuantouzhuensis. The type strains of M. rosea subsp. wuxiensis and M. rosea subsp. yuantouzhuensis are SIPI-226 and SIPI-207, respectively.
Li
X
X
Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, China.
Zhu
B
B
Gong
B
B
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics*; D007166:Immunosuppressive Agents / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D021521:Ribotyping; D012988:Soil Microbiology; D009626:Terminology as Topic
D004269:DNA, Bacterial; D007166:Immunosuppressive Agents; D012336:RNA, Ribosomal, 16S