[High efficiency gene transfer into breast cancer cells by pseudotyped retroviral vector].
OBJECTIVE
To investigate the efficiency of gene transfer mediated by pseudotyped retroviral vector in breast cancer, and to provide an useful vector for breast cancer gene therapy.


METHODS
We used a MuLV vector pseudotyped with the vesicular stomatitis virus glycoprotein(VSV-G) envelope to transduce breast cancer cells MDA-MB-435, To compare the transfer efficiency with MuLV.


RESULTS
The transfer efficiency is (92 +/- 12)% by using MuLV/VSV-G vector, (24 +/- 5)% by MuLV vector. The transfer efficiency of MuLV/VSV-G is 3.8 times that of MuLV vector.


CONCLUSIONS
Our results suggest that pseudotyped retroviral vector MuLV/VSV-G is a kind of high efficiency gene transfer vector in breast cancer cells.
Tang
L L
LL
Department of Oncology, Xiangya Hospital, Central South University, Changsha, 41008.
Yu
H
H
D001943:Breast Neoplasms / Q000473:pathology* / Q000628:therapy; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D012191:Retroviridae Proteins / Q000235:genetics; D014407:Tumor Cells, Cultured; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000378:metabolism*
C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D012191:Retroviridae Proteins; D014759:Viral Envelope Proteins