[Establishment of method for determining serum beta-hydroxybutyric acid and its meaning in diabetes ketosis acidism].
OBJECTIVE
This study was to establish enzyme-rate method for determining serum beta-hydroxybutyric acid, and to discuss its meaning in diabetes ketosis acidism.


METHODS
Enzyme-rate method was used to determine serum beta-hydroxybutyric acid in 60 cases of normals, 30 cases of diabetes, and 8 cases of ketosis acidism by Hitachi 7170A autochemistry analyzer.


RESULTS
The recovery rate is 108%, the linerity extent 0-4.0 mmol.L-1, the coefficient of variation within-run is 2.2% and day-to-day is 2.6%.


CONCLUSIONS
The enzyme-rate method for determining serum beta-hydroxybutyric acid save time, gives accurate results and has wide linerity, so it can used for diagnosis of diabetes ketosis acidism.
Yi
B
B
Department of Clinical Laboratory, Xiangya Hospital, Centeal South University, Changsha 410008, China.
Wei
G F
GF
Tang
Y
Y
D020155:3-Hydroxybutyric Acid / Q000097:blood*; D000328:Adult; D016883:Diabetic Ketoacidosis / Q000097:blood* / Q000150:complications; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged
D020155:3-Hydroxybutyric Acid