[Detection of pathogenic Edwarsiella tarda].
25 Edwardsiella tarda(Et) strains had been detected both on their viruslent factor Excellular Product (ECP), including the hemolysin and extracellular protease (ECPase), and on their pathogenicity to mice and Xiphophorus helleri. ECP was detected by Dot-ELISA with rabbit antiserum against ECP of reference strain JEL4. The results showed that the animal pathogenicity of Et had good correlation with its hemolysin other than with ECPase. The agreement between Dot-ELISA of JEL4 ECP and pathogenicity to animal was up to 100%. It was desirable to establish a detecting method, which only need detect the ECP with plate assay (PA) and Dot-ELISA, but needn't have animal experiment. Furthermore it is possible to develop a diagnosis kit of application to simplify the detecting procedure of pathogenic Et.
Xiong
Q
Q
Key Lab of Animal Disease Diagnostic and Immunology, Nangjing Agricultural University, Nanjing 210095, China.
Lu
C
C
D000818:Animals; D003532:Cyprinodontiformes / Q000382:microbiology; D020609:Edwardsiella tarda / Q000302:isolation & purification* / Q000472:pathogenicity; D010450:Endopeptidases / Q000032:analysis; D004756:Enterobacteriaceae Infections / Q000378:metabolism / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005393:Fish Diseases / Q000382:microbiology; D006460:Hemolysin Proteins / Q000032:analysis*; D051379:Mice; D014774:Virulence
D006460:Hemolysin Proteins; D010450:Endopeptidases