title: Evidence for discoordinate regulation of the HLA-DPB1 gene.
abstract: Characterization of cell lines derived from patients with type II bare lymphocyte syndrome, a pathological state in which the constitutive and inducible expression of HLA class II antigens is lacking, has permitted the identification of several trans-acting factors involved in the coordinated regulation of HLA class II genes. Although an increasing body of evidence has pointed to the existence of a discoordinate regulation of HLA class II loci, the mechanisms underlying such regulation are essentially unknown. In the present study, 45.EM2, a mutant lymphoblastoid cell line with a new pattern of HLA discoordinate expression is characterized. 45.EM2 expresses HLA-DR and -DQ but fails to express HLA-DP. The absence of HLA-DP expression in 45.EM2 is the result of a transcriptional defect, leading to a lack of DPB1 mRNA. By contrast, DPA1 transcription in this LCL is not impaired. The characteristics of 45.EM2 described here suggest the existence of a specific trans-acting factor involved in the control of DPB1 gene expression.
authors.affiliation: Departmento de Microbiología II, Universidad Complutense, Madrid, Spain.
authors.forename: M T
authors.initials: MT
authors.lastname: Coiras
authors.forename: A M
authors.initials: AM
authors.lastname: Alvarez-Barrientos
authors.forename: G
authors.initials: G
authors.lastname: Díaz
authors.forename: J
authors.initials: J
authors.lastname: Arroyo
authors.forename: M
authors.initials: M
authors.lastname: Sánchez-Pérez
mesh_terms: D002461:Cell Line, Transformed; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006682:HLA-DP Antigens / Q000235:genetics*; D059867:HLA-DP alpha-Chains; D059868:HLA-DP beta-Chains; D006801:Humans; D008156:Luciferases / Q000235:genetics; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D016511:Severe Combined Immunodeficiency / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D014162:Transfection
chemical_list: D006682:HLA-DP Antigens; D059867:HLA-DP alpha-Chains; D059868:HLA-DP beta-Chains; C071172:HLA-DPA1 antigen; C071170:HLA-DPB1 antigen; D012333:RNA, Messenger; D014157:Transcription Factors; D008156:Luciferases