title: [Preparation of immobilized penicillin V acylase from Fusarium oxysporum and its properties].
abstract: Extracellular penicillin V acylase from Fusarium oxysporum FP941 was partially purified by means of adsorption on, and elution from gamma-alumina and (HN4)2SO4 fractional precipitation. The enzyme was immobilized on acrylic polymer support linkaged with covalent bond. The activity of wet immobilized enzyme was 217 IU/g, The activity yield of Immobilized enzyme was 53%. Optimum pH and temperature for immobilized enzyme action wer 8.0 and 55 degrees C, respectively. The enzyme was stable below 50 degrees C and pH 4-11. The activity of the immobilized enzyme remained 90% after it was reused 25 times.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
authors.forename: Z
authors.initials: Z
authors.lastname: Dong
authors.forename: F
authors.initials: F
authors.lastname: Cui
mesh_terms: D004800:Enzymes, Immobilized / Q000378:metabolism*; D005670:Fusarium / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D010399:Penicillin Amidase / Q000378:metabolism*; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D010399:Penicillin Amidase