X-ray crystallographic characterization and phasing of a fucose-specific lectin from Aleuria aurantia.
A fucose-specific lectin from Aleuria aurantia was crystallized in its native form and was also cocrystallized with HgCl(2). Crystallization was performed using the sitting-drop vapour-diffusion method with ammonium sulfate as a precipitant. Both the Hg-free native crystals and the Hg cocrystals belong to the hexagonal space group P6(5)22. The unit-cell parameters for the Hg-free form are a = 84.0, c = 250.1 A and for the Hg cocrystals are a = 83.9, c = 254.3 A. Both forms of the crystals diffract X-rays to 2.3 A resolution and are suitable for high-resolution crystal structure determination. Initial phasing was successfully performed by the MAD method using the Hg cocrystals and the electron density obtained was good enough for model building.
Fujihashi
Masahiro
M
Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Peapus
Diane H
DH
Nakajima
Eijiro
E
Yamada
Tsuyoshi
T
Saito
Jun Ichi
JI
Kita
Akiko
A
Higuchi
Yoshiki
Y
Sugawara
Yoko
Y
Ando
Akikazu
A
Kamiya
Nobuo
N
Nagata
Yoshiho
Y
Miki
Kunio
K
D001203:Ascomycota / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods; D005656:Fungal Proteins / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*; D008627:Mercuric Chloride / Q000737:chemistry; D055672:Static Electricity
D005656:Fungal Proteins; D037102:Lectins; C064549:lectin, Aleuria aurantia; D008627:Mercuric Chloride