The fusion core complex of the peste des petits ruminants virus is a six-helix bundle assembly.
We describe the properties of the two heptad repeats (HR1 and HR2) of the Peste des petits ruminants virus (PPRV) fusion protein (F) to obtain insights into the mechanism by which these repeats influence PPRV-mediated cell fusion. Both HR1 and HR2 inhibit PPRV-mediated syncytia formation in Vero cells in vitro. Of these, HR2 was found to be more effective than HR1. We studied the mechanism of fusion inhibition by these two repeats by using various biophysical and biochemical methods either separately or together. CD spectral analysis of these repeats revealed that the alpha-helical content of HR1 and HR2 when used together is higher than that of their simulated spectrum in the mixture, suggesting the formation of a highly structured complex by these repeats. Protease protection assays confirmed that such a complex is highly stable. Electrospray mass spectrometry of protease-digested products of the HR1-HR2 complex showed protection of fragments corresponding to both HR1 and HR2 sequences involved in complex formation. By employing size-exclusion chromatography and chemical cross-linking experiments, we show that three units each of HR1 and HR2 form a complex in which HR1 is a trimer and HR2 is a monomer. Homology-based three-dimensional modeling of this complex showed that HR1 and HR2 together form a six-helix and trimeric coiled-coil bundle. In this model, the HR1 trimer forms the core whereas HR2, while interacting with HR1 in an antiparallel orientation, forms a two-stranded coiled-coil structure and lies at the periphery of the structure. These results are discussed in the context of a common fusion mechanism among paramyxoviruses.
Rahaman
Abdur
A
Department of Microbiology and Cell Biology, Molecular Biophysics Unit, and Department of Physics, Indian Institute of Science, Bangalore 560012, India.
Srinivasan
N
N
Shamala
N
N
Shaila
M S
MS
D020816:Amino Acid Motifs / Q000235:genetics / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000502:physiology; D002522:Chlorocebus aethiops; D019286:Endopeptidase K / Q000378:metabolism; D015726:Giant Cells / Q000502:physiology / Q000821:virology; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D006868:Hydrolysis; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D018107:Peste-des-petits-ruminants virus / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D017433:Protein Structure, Secondary / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D020449:Repetitive Sequences, Amino Acid / Q000235:genetics / Q000502:physiology; D029082:Sendai virus / Q000737:chemistry; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D019065:Virus Assembly / Q000502:physiology*
D000998:Antiviral Agents; C079748:F protein, Rinderpest virus; D006023:Glycoproteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; D014760:Viral Fusion Proteins; D019286:Endopeptidase K