title: [The relationship between fMLP induced neutrophil respiratory burst and the apoptosis of neutrophil].
abstract: The relationship between apoptosis of neutrophils and the change of their intracellular free Ca2+ concentration [Ca2+]i was studied. FMLP and A23187 were used to elevate the [Ca2+]i while BAPTA was used to deplete it. Fluorescence microscope, flow cytometry and gel electrophoresis were used to study the percentage of cell apoptosis and the change of f-actin during apoptosis. The results showed that the apoptosis was obviously inhibited by fMLP and A23187, while accelerated by BAPTA. The detection of f-actin showed that the f-actin depolymerized obviously during apoptosis. The elevation of [Ca2+]i inhibit the actin depolymerization while depletion of [Ca2+]i accelerated it. This result indicated that the apoptosis of neutrophil was obviously inhibited by [Ca2+]i elevation but accelerated by [Ca2+]i depletion.
authors.affiliation: Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101.
authors.forename: T H
authors.initials: TH
authors.lastname: Hu
authors.forename: L
authors.initials: L
authors.lastname: Bei
authors.forename: Y F
authors.initials: YF
authors.lastname: Huang
authors.forename: X
authors.initials: X
authors.lastname: Shen
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D016897:Respiratory Burst / Q000187:drug effects*
chemical_list: D000001:Calcimycin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002118:Calcium