title: [The isolation and identification of pathogenic bacteria from rampant caries in children].
abstract: OBJECTIVE
  In order to prevent rampant caries in children, the predominant pathogenic bacteria of rampant caries in children were defined.
  
  
  METHODS
  The pathogenic bacteria of rampant caries were isolated and identified in 30 children with rampant caries, aged from 2 to 5 years old. Samples were collected from the caries of labial surfaces of maxillary anterior deciduous teeth, including caries lesions and caries-free smooth enamel surfaces. Plaque were obtained from the cervical third areas of the maxillary labial anterior deciduous tooth surfaces in children without rampant caries and caries-free children and served as controls respectively. The isolates were identified by colony morphology, biochemical tests and DNA base contents (mol percent G + C).
  
  
  RESULTS
  The prevalence of Streptococcus mutans (S. mutans) and Streptococcus sobrinus (S. sobrinus) in the plaque of caries lesions and the proportions of S. mutans and S. sobrinus in the plaque of 2 sampling sites of children with rampant caries were all significantly higher than those of children without rampant caries and caries-free children (P < 0.05).
  
  
  CONCLUSIONS
  S. mutans and S. sobrinus are predominant pathogenic bacteria in rampant caries in children.
authors.affiliation: Department of Preventive Dentistry, School of Stomatology, Shanghai Second Medical University.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Feng
authors.forename: Y
authors.initials: Y
authors.lastname: Pan
authors.forename: W
authors.initials: W
authors.lastname: Chen
mesh_terms: D002648:Child; D003731:Dental Caries / Q000382:microbiology*; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D013295:Streptococcus mutans / Q000302:isolation & purification*; D016986:Streptococcus sobrinus / Q000302:isolation & purification*