[Determination of salicin in extract of willow bark by high performance liquid chromatography].
An HPLC method for the determination of salicin in extract of willow bark is described. Chromatographic analysis was carried out on a Kromasil C18, 5 microns column(4.6 mm i.d. x 250 mm) with methanol-0.01 mol/L KH2PO4 buffer (pH 4.01) (15:85, volume ratio) as mobile phase. The detection wavelength was 265 nm. Salicin was extracted from samples with methanol-water(50:50, volume ratio), and centrifuged. Ten microL of supernatant were injected. The average recoveries were from 96.1% to 101.2% (n = 5), and the relative standard deviation (RSD) was 1.43%. The method is simple, rapid and accurate.
Li
L S
LS
Institute of Applied Chemistry, Nanchang University, Nanchang 330047, China.
Huang
W D
WD
He
Q
Q
Ye
S
S
D001592:Benzyl Alcohols / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D032108:Salix / Q000737:chemistry*
D001592:Benzyl Alcohols; D005960:Glucosides; D010936:Plant Extracts; C005696:salicin