Molecular cytogenetic aberrations in autosomal dominant polycystic kidney disease tissue.
Autosomal dominant polycystic kidney disease (ADPKD) is a genetically heterogeneous disorder characterized by focal cyst formation from any part of the nephron. The molecular bases include germinal mutation of either PKD1 or PKD2 genes, enhanced expression of several protooncogenes, alteration of the TGF-alpha/EGF/EGF receptor (EGFR) axis, and disturbed regulation of proliferative/apoptosis pathways. To identify new locations of ADPKD related oncogenes and/or tumor suppressor genes (TSG), comparative genomic hybridization (CGH) and loss of heterozygosity (LOH) analyses were performed for a series of individual cysts (n = 24) from eight polycystic kidneys. By CGH, imbalances were detected predominantly on chromosomes 1p, 9q, 16p, 19, and 22q in all tissues. DNA copy number gain was seen on chromosomes 3q and 4q in five samples. The CGH data were supplemented by LOH analysis using 83 polymorphic microsatellite markers distributed along chromosomes 1, 9, 16, 19, and 22. The highest frequency of LOH was found on the 1p35-36 and 16p13.3 segments in cysts from seven samples. Allelic losses on 9q were detected in six, whereas deletions at 19p13 and 22q11 bands were observed in three polycystic kidneys. These results indicate that the deleted chromosomal regions may contain genes important in ADPKD initiation and progression.
Gogusev
Jean
J
INSERM U507, Hôpital Necker, Paris, France. gogusev@necker.fr
Murakami
Ichiro
I
Doussau
Mireille
M
Telvi
Louise
L
Stojkoski
Alexandre
A
Lesavre
Philippe
P
Droz
Dominique
D
D002869:Chromosome Aberrations; D002875:Chromosomes / Q000235:genetics; D020732:Cytogenetic Analysis; D004247:DNA / Q000235:genetics; D005260:Female; D018628:Gene Dosage; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D009693:Nucleic Acid Hybridization; D016891:Polycystic Kidney, Autosomal Dominant / Q000235:genetics* / Q000473:pathology
D004247:DNA