title: Bacterial contamination in postmortem bone donors.
abstract: We analyzed factors influencing the contamination rate of allografts and blood samples obtained from postmortem bone donors. 5,710 allografts were harvested, from 550 donors of which 3,164 (55%) were swab culture negative. Blood cultures were positive in 140 donors (26%). The risk of graft contamination increased with each extra team member (Odds Ratio 1.9). It was also higher with organisms of greater virulence in donors with a positive blood culture (OR 3.5). The risk of blood contamination increased per hour postmortem (OR 1.1) and the same increase was seen with organisms of high virulence. In donors with multiple trauma, the risk of blood contamination with organisms of high virulence was greater (OR 8.2), but smaller in donors with preceding organ procurement (OR 0.1). To minimize the bacterial load, donors should be obtained in operating rooms, using aseptic techniques with only a few personnel for procurement. The postmortem time should be kept to a minimum. The procurement cultures from donors with multiple trauma should be carefully interpreted. Blood cultures should be taken into account, since these can help to find contamination not detected by swab cultures.
authors.affiliation: Netherlands Bone Bank Foundation, Leiden. info@vehmeijer.nl
authors.forename: Stephan
authors.initials: S
authors.lastname: Vehmeyer
authors.forename: Julius
authors.initials: J
authors.lastname: Wolkenfelt
authors.forename: Ruud
authors.initials: R
authors.lastname: Deijkers
authors.forename: Pieter
authors.initials: P
authors.lastname: Petit
authors.forename: Ronald
authors.initials: R
authors.lastname: Brand
authors.forename: Rolf
authors.initials: R
authors.lastname: Bloem
mesh_terms: D001424:Bacterial Infections / Q000209:etiology* / Q000382:microbiology; D001769:Blood / Q000382:microbiology*; D016025:Bone Transplantation / Q000009:adverse effects*; D001842:Bone and Bones / Q000382:microbiology*; D002102:Cadaver; D002423:Cause of Death; D005260:Female; D006801:Humans; D008297:Male; D016017:Odds Ratio; D011424:Propionibacterium / Q000302:isolation & purification; D012044:Regression Analysis; D012307:Risk Factors; D013291:Streptococcus / Q000302:isolation & purification; D013997:Time Factors; D014019:Tissue Donors; D014184:Transplantation, Homologous