title: Localization and measurement of extracellular plant galactosidases.
abstract: A simple, rapid and sensitive procedure for the identification and determination of plant extracellular alpha-galactosidase and beta-galactosidase is described using callus cultures and seedlings from tomato. Synthetic substrates (1-naphthyl- and p-nitrophenyl-alpha-D- and beta-D-galactopyranosides) were used for the identification and determination of intracellular and extracellular activity of alpha-galactosidase and beta-galactosidase, respectively. Many iminosugars or azasugars are strong glycosidase inhibitors and some of them show promising chemotherapeutic effects against viral diseases, and are potentially antidiabetic agents, as well as antitumor agents. These facts initiated our interest in a rapid and sensitive assay to determine activity of alpha-galactosidase and beta-galactosidase in plant tissues. The results presented here show the potential of the assay of the activity of intracellular and extracellular galactosidases of plant origin in inhibitory and/or biotechnological studies.
authors.affiliation: Garden of Medicinal Plants, Faculty of Pharmacy, Bratislava, Slovak Republic. korenova@fpharm.uniba.sk
authors.forename: Ján
authors.initials: J
authors.lastname: Stano
authors.forename: Peter
authors.initials: P
authors.lastname: Kovács
authors.forename: Karol
authors.initials: K
authors.lastname: Micieta
authors.forename: Klaus
authors.initials: K
authors.lastname: Neubert
authors.forename: Herbert
authors.initials: H
authors.lastname: Tintemann
authors.forename: Marcela
authors.initials: M
authors.lastname: Korenová
mesh_terms: D002478:Cells, Cultured; D005110:Extracellular Space / Q000201:enzymology*; D006651:Histocytochemistry; D007424:Intracellular Fluid / Q000201:enzymology; D018551:Solanum lycopersicum / Q000166:cytology / Q000201:enzymology*; D036226:Seedlings / Q000166:cytology / Q000201:enzymology; D000519:alpha-Galactosidase / Q000032:analysis / Q000378:metabolism*; D001616:beta-Galactosidase / Q000032:analysis / Q000378:metabolism*
chemical_list: D000519:alpha-Galactosidase; D001616:beta-Galactosidase