title: Laparoscopic fluorometry: a new minimally invasive tool for investigation of the intestinal microcirculation.
abstract: The aim of this study was to develop and establish a new system of laparoscopic fluorometry for the purpose of investigating the intestinal microcirculation. In 25 pigs (German Landrace, 16-25 kg body weight), ischemia was established in two segments (A, irreversible; B, reversible ischemia; C, internal control) of the small intestine by a laparoscopic technique. Microcirculation in the segments was assessed by laparoscopy at a second-look operation 24 h later by means of the fluorescence system Endoscan. The fluorescence of the three bowel segments was measured by arbitrary dye fluorescence units (DFU) 15 min after starting reperfusion, before and after injection of sodium fluorescein (NaFlu, 0.25 mg/kg body weight). The dividing line between viable and nonviable bowel tissue was established from the inflow and outflow rates of NaFlu with the aid of ROC (receiver operating characteristic) curves. The specificity and sensitivity of the new method were evaluated by correlating the results with the viability of each intestinal segment as predicted by three laparoscopically experienced surgeons and by histological examination. By means of the calculated separation sharpness (fluorescence index at 2 min >0.5, outflow factor of NaFlu at 10 min >20%), the overall predictions of intestinal viability in all 25 animals achieved a sensitivity of 93.5% and a specificity of 94.1% by laparoscopic fluorometry, versus a sensitivity of 70.8% and a specificity of 87.5% for the prediction of bowel viability by ordinary laparoscopic technique. Used as an adjunct to conventional laparoscopy, laparoscopic fluorometry brought significant gains in sensitivity and specificity in the distinction between reversible and irreversible intestinal ischemia.
authors.affiliation: Department of General, Visceral, and Vascular Surgery, Herz-Jesu-Hospital, Muenster-Hiltrup, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Horstmann
authors.forename: D
authors.initials: D
authors.lastname: Palmes
authors.forename: D
authors.initials: D
authors.lastname: Rupp
authors.forename: G
authors.initials: G
authors.lastname: Hohlbach
authors.forename: H U
authors.initials: HU
authors.lastname: Spiegel
mesh_terms: D000818:Animals; D005260:Female; D005470:Fluorometry; D007422:Intestines / Q000098:blood supply*; D010535:Laparoscopy; D008297:Male; D008833:Microcirculation; D012039:Regional Blood Flow; D013552:Swine; D018608:Ultrasonography, Doppler