[Phylogenetic analysis of two rhizobia new groups].
On the basis of numerical taxonomy, SDS-PAGE whole-cell protein analysis, 16S rDNA PCR-RFLP and DNA-DNA hybridization, the full length of 16SrDNA of two strains (XJ96060, XJ96408) were sequenced and compared with the 16S rDNA sequences of other members of the alpha 2 subclass of the Proteobacteria available from GenBank. An unrooted tree was produced to determine the phylogenetic relationships of two groups. The results showed that all strains were clustered into 6 branches, they were Sinorhizobium, Mesorhizobium, Agrobacterium-Rhizobium, Bradyrhizobium. Rhizobium, Azorhizobium. Group 1, 2 fell into Sinorhizobium branch.
Yan
A
A
China Agriculture University, Biological College, Beijing 100094.
Chen
W
W
D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology*; D029902:Medicago / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000145:classification* / Q000235:genetics*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S