[Determination of 17-hydroxycorticosteroid by capillary zone electrophoresis].
OBJECTIVE
To detect and dissociate 17-hydroxycorticosteroids(17-OHCS) in urine by using high performance capillary zone electrophoresis(CZE) so as to solve the problem that phenolic compounds interfere with the determination of urine 17-OHCS by chromatographic-spectrophotometry.


METHODS
Pigments were absorbed from samples by activated kaolin in which the internal standard was theophyline. Urine 17-OHCS was absorbed by neutral styrene-divinylbenzene resin. Thus pigments and phenolic compounds were separated roughly. Then 17-OHCS was washed down by alcohol and separated and detected by capillary zone electrophoresis.


RESULTS
Urinary 17-OHCS was separated successfully. Urinary 17-OHCS had a good linearity in the range of 2-50 mg.L-1 (r = 0.994). The average recovery rate was 96.17%. The relative standard deviation was less than 5%. The consistence detection limit was 0.3 mg.L-1.


CONCLUSIONS
Compared with chromatographic-spectrophotometry, the high performance capillary electrophoresis has much less interference and is more simple, rapid, and accurate.
Jing
Z Z
ZZ
Central Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China.
Tang
F Q
FQ
Xiao
Z Q
ZQ
D015065:17-Hydroxycorticosteroids / Q000652:urine*; D019075:Electrophoresis, Capillary; D006801:Humans
D015065:17-Hydroxycorticosteroids