[Vascular bundle specific expression of iaaL gene affects the generation frequencies of transgenic tobacco].
A vascular bundles specific expressing vector pBAL1 with a promoter AQ630 from rice phenylalanine ammonialyase gene and a gene encoding indoleacetic-lysine synthytase from Pseudomonas syringae subsp. savastanoi was constructed. Affirmed by Southern blotting and RTPCR analysis, the AQ630-iaaL transgenic plants show increasing shoots-regeneration frequency of young stem explants on hormone-free 1/2 MS medium and lower sensibility to IAA when roots were induced from the root explants on the media containing different concentrations of IAA compared to untransformed plants.
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National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology, Academia Sinica, Shanghai 200032.
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D046508:Culture Techniques; D015870:Gene Expression; D010453:Peptide Synthases / Q000235:genetics*; D018517:Plant Roots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development
D010453:Peptide Synthases; C048911:indoleacetic acid-lysine synthetase