title: Two budding yeast RAD4 homologs in fission yeast play different roles in the repair of UV-induced DNA damage.
abstract: We have identified two fission yeast homologs of budding yeast Rad4 and human xeroderma pigmentosum complementation group C (XP-C) correcting protein, designated Rhp4A and Rhp4B. Here we show that the rhp4 genes encode NER factors that are required for UV-induced DNA damage repair in fission yeast. The rhp4A-deficient cells but not the rhp4B-deficient cells are sensitive to UV irradiation. However, the disruption of both rhp4A and rhp4B resulted in UV sensitivity that was greater than that of the rhp4A-deficient cells, revealing that Rhp4B plays a role in DNA repair on its own. Fission yeast has two pathways to repair photolesions on DNA, namely, nucleotide excision repair (NER) and UV-damaged DNA endonuclease-dependent excision repair (UVER). Studies with the NER-deficient rad13 and the UVER-deficient (Delta)uvde mutants showed the two rhp4 genes are involved in NER and not UVER. Assessment of the ability of the various mutants to remove cyclobutane pyrimidine dimers (CPDs) from the rbp2 gene locus indicated that Rhp4A is involved in the preferential repair of lesions on the transcribed DNA strand and plays the major role in fission yeast NER. Rhp4B in contrast acts as an accessory protein in non-transcribed strand (NTS) repair.
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Fukumoto
authors.forename: Hideki
authors.initials: H
authors.lastname: Hiyama
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yokoi
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Nakaseko
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yanagida
authors.forename: Fumio
authors.initials: F
authors.lastname: Hanaoka
mesh_terms: D018797:Cell Cycle Proteins / Q000502:physiology*; D004249:DNA Damage; D017931:DNA Primers / Q000737:chemistry; D004260:DNA Repair; D004271:DNA, Fungal / Q000528:radiation effects*; D004268:DNA-Binding Proteins / Q000502:physiology*; D005656:Fungal Proteins / Q000502:physiology*; D018390:Gene Targeting; D009154:Mutation; D016133:Polymerase Chain Reaction; D004718:Saccharomycetales / Q000235:genetics; D012568:Schizosaccharomyces / Q000528:radiation effects*; D029702:Schizosaccharomyces pombe Proteins / Q000502:physiology*; D017384:Sequence Deletion; D014158:Transcription, Genetic; D011503:Transglutaminases / Q000502:physiology*; D014466:Ultraviolet Rays
chemical_list: D018797:Cell Cycle Proteins; D017931:DNA Primers; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C082463:RAD4 protein, S pombe; C490845:RHP23 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; C088086:rad26 protein, S pombe; D011503:Transglutaminases