[Allele-specific chromosome 9p deletion in oral cancer].
OBJECTIVE
The aim of this study was to investigate the relationship between loss of heterozygosity (LOH) on chromosome 9p and the pathogenesis of oral squamous cell carcinoma (OSCC).


METHODS
A total of 24 human OSCC specimens were analyzed for LOH on chromosome 9p using 8 microsatellite markers by means of polymerase chain reaction.


RESULTS
In 24 cases of OSCC, LOH on chromosome 9p was identified in 10 of 24 cases (41.67%) with at least one marker. The main LOH were found on 9p21 at locus D9S171 (21.05%) and D9S304 (10.00%). There were also a deletion on 9p22-23 at locus D9S168 (22.22%) and D9S162 (15.38%). However, there was no statistically significant correlation between LOH at these loci with such clinical parameters as pathological types, tumor size and lymph-node metastasis. Microsatellite instability (MSI) is rare for any of the 8 markers in 24 OSCC. No MSI could observed using the common criteria for defining MSI-its detection in two or more markers.


CONCLUSIONS
We found that high frequency of LOH occurred at 9p21-23 band. Their results indicate that more than one tumor suppressor genes at chromosome 9p21-23 region related to a subset of OSCC, while MSI might not be a crucial event.
Xiao
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Cancer Center, First Affiliated Hospital, West China University of Medical Sciences.
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Luo
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D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats / Q000235:genetics; D009062:Mouth Neoplasms / Q000235:genetics*