title: [Production of vitamin C precursor--2-keto-L-gulonic acid from D-sorbitol by mixed culture of microorganisms].
abstract: Gluconobacter oxydans SCB329 only produce a little amount of 2-Keto-L-gulonic acid(2-KLG) from D-Sorbitol when growing alone; while Gluconobacter sp. SCB110 can transform D-Sorbitol to L-Sorbose and can not produce 2-KLG. 2-Keto-L-gulonic acid, the precursor of L-Ascorbic acid (Vitamin C) synthesis, was prepared directly with a high efficiency from D-Sorbitol by mixed culture of microorganism, which comprised Gluconobacter sp. SCB110 and Gluconobacter oxydans SCB329. The fermentation product from the mixed culture broth in the D-Sorbotol-containing medium was identified as 2-Keto-L-gulonic acid by HPLC, elementary analysis and infra-red adsorption spectrum.
authors.affiliation: Shanghai Research Center of Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China.
authors.forename: G
authors.initials: G
authors.lastname: Yin
authors.forename: W
authors.initials: W
authors.lastname: Lin
authors.forename: C
authors.initials: C
authors.lastname: Qiao
authors.forename: Q
authors.initials: Q
authors.lastname: Ye
mesh_terms: D018920:Coculture Techniques; D005285:Fermentation; D020576:Gluconobacter / Q000378:metabolism*; D020600:Gluconobacter oxydans / Q000378:metabolism*; D013012:Sorbitol / Q000378:metabolism*; D013400:Sugar Acids / Q000378:metabolism*
chemical_list: D013400:Sugar Acids; D013012:Sorbitol; C011491:provitamin C