3'-processing of yeast tRNATrp precedes 5'-processing.
Previous analyses of eukaryotic pre-tRNAs processing have reported that 5'-cleavage by RNase P precedes 3'-maturation. Here we report that in contrast to all other yeast tRNAs analyzed to date, tRNA(Trp) undergoes 3'-maturation prior to 5'-cleavage. Despite its unusual processing pathway, pre-tRNA(Trp) resembles other pre-tRNAs, showing dependence on the essential Lsm proteins for normal processing and efficient association with the yeast La homolog, Lhp1p. tRNA(Trp) is also unusual in not requiring Lhp1p for 3' processing and stability. However, other Lhp1p-independent tRNAs, tRNA(2)(Lys) and tRNA(1)(Ile), follow the normal pathway of 5'-processing prior to 3-processing.
Kufel
Joanna
J
Wellcome Trust Centre for Cell Biology, King's Buildings, The University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK.
Tollervey
David
D
D063211:N-Terminal Acetyltransferase C; D012323:RNA Processing, Post-Transcriptional; D012364:RNA, Transfer, Trp; D016601:RNA-Binding Proteins / Q000378:metabolism; D017414:Ribonucleoprotein, U4-U6 Small Nuclear / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*
C117009:NAA38 protein, human; D012364:RNA, Transfer, Trp; D016601:RNA-Binding Proteins; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; D063211:N-Terminal Acetyltransferase C