Detection of 3-hydroxykynurenine in a plant pathogenic fungus.
A redox-active compound has been purified from the barley powdery mildew fungus Blumeria ( Erysiphe ) graminis f. sp. hordei. A combination of spectrophotometry, MS and NMR has identified it as 3-hydroxykynurenine (3OHKyn). This compound, never previously detected in any fungus or pathogen, is best known for its role in vertebrate cataracts. It is found abundantly in developing and germinating spores and also in runner hyphae. Two roles for 3OHKyn are discussed: first, the presence of active oxygen species would enable 3OHKyn to cross-link the spore chemically with the plant. Secondly, it may be acting as an UV protectant and an antioxidant.
Wilson
T J Greer
TJ
Division of Science and Engineering, Australian Centre for Necrotrophic Fungal Pathogens, Murdoch University, Perth, WA 6150, Australia. gwilson@central.murdoch.edu.au
Thomsen
Karl Kristian
KK
Petersen
Bent O
BO
Duus
Jens Ø
JØ
Oliver
Richard P
RP
D001203:Ascomycota / Q000472:pathogenicity*; D007737:Kynurenine / Q000031:analogs & derivatives* / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D010944:Plants / Q000382:microbiology*
C005045:3-hydroxykynurenine; D007737:Kynurenine