[Protein A tangential flow membrane chromatographic column used for immunoadsorption therapy].
Tangential flow membrane chromatographic column is a new model of immunoadsorption therapy from blood. The results showed that the structure of the column and the flowing channel had a little damages for blood cells. The relation of flow-rate and backpressure with water, plasma and blood individually as the fluid flowing through the column has been studied. It was observed that backpressures increased with fluid viscosity, when flow-rate of blood was 120 mL/min and the backpressure reached 0.093 MPa; The adsorption capacity of protein A of the column for IgG from human plasma has been measured. When plasma and blood individually circulated through the column for 1 h, the column with 139 mg protein A immobilized on the matrix (6 mg protein A/g dry matrix) adsorbed 553 mg IgG (23.8 mg IgG/g dry matrix) from human plasma and 499.4 mg IgG (21.5 mg IgG/g dry matrix) from human blood respectively. The circulation time had a great influence on IgG adsorption capacity; but the circulation rate had a little influence on IgG adsorption capacity. The result of extracoporeal blood perfusion for dog showed that the Protein A column has good blood biocompatibility.
Jia
L
L
National Chromatographic R. & A. Center, Laboratory of Chromatography, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116012.
Yang
L
L
Zou
H
H
Zhang
Y
Y
Zhao
J
J
Fan
C
C
Sha
L
L
D002845:Chromatography / Q000379:methods; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007163:Immunosorbent Techniques; D013205:Staphylococcal Protein A
D007074:Immunoglobulin G; D013205:Staphylococcal Protein A