[Isolation of a lipase-producing Pseudomonas strain and optimization of its fermentation conditions].
A lipase-producing bacterium strain was isolated from soil and was identified as Pseudomonas sp.. Its lipase yield was improved 2.25-fold by combined treatment of UV irradiation and NTG. The lipase fermentation condition for the mutant strain was optimized with Plackett-Burman design and Response Surface Analysis (RSA), and the formula of the optimum medium suitable for industrial scale fermentation was thereby established. A maximum yield of 87.5 U/ml was obtained.
Gao
X
X
Wuxi University of Light Industry, Wuxi 214036.
Zhang
K
K
Cao
S
S
D003470:Culture Media; D008049:Lipase / Q000096:biosynthesis*; D011549:Pseudomonas / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification*; D012988:Soil Microbiology
D003470:Culture Media; D008049:Lipase