[Nucleotide sequence analysis of an antibiotic biosynthesis gene of Streptomyces globisporus].
Antitumor antibiotic C-1027 produced by Streptomyces globisporus has very high biological activity both in vivo and in vitro. Research works showed that one of biosynthesis gene of C-1027 is in the F2 DNA fragment. The plasmid pUC18 was used as vector to subclone the F2 DNA fragment. The nucleotide sequence analysis for F2 DNA fragment was carried out. Results showed that there is an open reading frame encoding for 122 amino acids. According to EMBO and GeneBanks data, this sequence may be a new one.
Mao
X
X
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050.
Li
Y
Y
D000595:Amino Acid Sequence; D000617:Aminoglycosides / Q000096:biosynthesis* / Q000737:chemistry; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis* / Q000737:chemistry; D001483:Base Sequence; D053281:Enediynes; D008969:Molecular Sequence Data; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
D000617:Aminoglycosides; D000903:Antibiotics, Antineoplastic; D053281:Enediynes; C058024:C 1027