Silver particles enhance emission of fluorescent DNA oligomers.
Here we describe a new opportunity in methodology for increasing the detectability of fluorescently labeled DNA on solid substrates. We show that the use of glass substrates coated with metallic silver particles results in an approximate 5-fold increase in the intensity of Cy3- or Cy5-labeled DNA oligomers. Proximity to these silver particles also increases the photostability of Cy3- and Cy5-labeled oligomers. These results suggest the use of DNA array substrates with silver particles for increased sensitivity in genetic analysis.
Lakowicz
Joseph R
JR
University of Maryland School of Medicine, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA. lakowicz@cfs.umbi.umd.edu
Malicka
Joanna
J
Gryczynski
Ignacy
I
D002232:Carbocyanines / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry; D011786:Quality Control; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*; D020349:Surface Plasmon Resonance
D002232:Carbocyanines; D005456:Fluorescent Dyes; D009841:Oligonucleotides; C089187:cyanine dye 3; C085321:cyanine dye 5; D012834:Silver; D004247:DNA