title: Use of taurine as antioxidant in resuscitating livers from non-heart-beating donors by gaseous oxygen persufflation.
abstract: In this study, the importance of antioxidants during oxygenated liver preservation has been investigated. Livers were excised from rats after 60 min of cardiac arrest and stored for 24 h at 4 degrees C in University of Wisconsin solution (UW). Gaseous oxygen was applied to the livers during the storage period via the caval vein after superoxide dismutase (SOD, 600 U/mL), taurine (0.5 mg/mL), or no antioxidant was applied to the graft with the rinse solution. It was shown that oxygen persufflation significantly enhanced the viability of livers during cold preservation only in combination with SOD or taurine, which were both equally effective in reducing lipid peroxidation, enzyme release, and vascular resistance upon postischemic reperfusion. An increase in hepatic bile production was also observed.
authors.affiliation: Surgical Research Divison, Clinic of Surgery, University of Bonn, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Lauschke
authors.forename: Martina
authors.initials: M
authors.lastname: Kötting
authors.forename: Susanne
authors.initials: S
authors.lastname: Akbar
authors.forename: Thomas
authors.initials: T
authors.lastname: Minor
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D006323:Heart Arrest; D007511:Ischemia / Q000188:drug therapy* / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000098:blood supply / Q000378:metabolism / Q000601:surgery; D008102:Liver Circulation; D016031:Liver Transplantation; D010100:Oxygen / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013654:Taurine / Q000494:pharmacology*; D014019:Tissue Donors; D014655:Vascular Resistance
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D013654:Taurine; D007770:L-Lactate Dehydrogenase; D010100:Oxygen