[Effect of danggui buxue decoction on proliferation and expression of intercellular adhesion molecule-1 in human umbilical vein endothelial cells].
OBJECTIVE
To investigate the effect of Danggui Buxue Decoction (DBD) on proliferation and expression of intercellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs).


METHODS
After treating HUVECs with various concentrations of DBD, we analyzed the proliferation phase of HUVECs and the expression of ICAM-1 using flow cytometry.


RESULTS
It was found that DBD could promote the proliferation of HUVECs and the expression of ICAM-1. The expression of CD54 (ICAM-1) was significantly increased in the experiment groups as compared with that in the control group (P < 0.05). In addition, cell cycle analysis revealed more synthetic period cells in the experiment groups than in control group (P < 0.05).


CONCLUSIONS
DBD can promote the proliferation of HUVECs and Increase the expression ICAM-1.
Wu
Y
Y
Department of Histology and Embryology, School of Basic Medical Sciences, WCUMS, Chengdu 610041, China.
Zhu
B
B
D029971:Angelica sinensis / Q000737:chemistry*; D027885:Astragalus propinquus / Q000737:chemistry*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D005260:Female; D006397:Hematinics / Q000494:pharmacology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D014471:Umbilical Veins / Q000166:cytology
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D006397:Hematinics; D018799:Intercellular Adhesion Molecule-1