[A study on mRNA expression levels of integrin beta 1 during the healing process of mandibular fractures].
OBJECTIVE
The aim of this study was to investigate the spatial and temporal mRNA expression changes of integrin beta 1 during the healing process of mandibular fractures.


METHODS
The in situ hybridization was performed using digoxigenin-labeled integrin beta 1 mRNA oligonucleotide probe. During the healing process of mandibular fractures, the staining pattern of beta 1 mRNA was examined both spatially and time-dependently.


RESULTS
Integrin beta 1 was widely present in the cells of the fractured bone, especially in osteoblasts. The positive staining was observed in the cytoplasm and nucleolus of osteoblasts. After 7 days of fracture, the expression level of integrin beta 1 increased. The peak appeared between the 14th and the 30th day, and almost recovered to normal between the 60th and 90th day.


CONCLUSIONS
During the healing process of mandibular fracture, the expression level of integrin beta 1 in bone, especially in osteoblasts rose. It is suggested that integrin beta 1 may play an important role in the fracture healing process.
Li
S
S
College of Stomatology, West China University of Medical Sciences.
Liu
S
S
Tian
W
W
Cheng
G
G
Liu
L
L
D000818:Animals; D005260:Female; D017102:Fracture Healing; D017403:In Situ Hybridization; D019012:Integrin beta1 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008337:Mandibular Fractures / Q000378:metabolism*; D010006:Osteoblasts / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits
D019012:Integrin beta1; D012333:RNA, Messenger