[Immune responses of a recombined live Salmonella typhimurium SL3261 expressing a multi-epitope antigen of HCV].
A multi-epitope antigen gene of hepatitis C virus(HCV) was fused to beta-galactosidase gene and introduced into attenuated Salmonella typhimurium SL3261 to construct HCV recombined live vaccine candidate SL3261 (pWR/PCX). when the oral live bacteria were used to immunize mice or rabbits, specific anti-GZ-PCX IgG was detected at week 6 and the strongest antibody responses happened at week 12 at a titer of 1:800 and 1:25,600 in mice and rabbits, respectively, which showed significant difference compared with those of SL3261 and blank controls. Anti-GZ-PCX sIgA in mice's intestine and anti-LPS antibody in sera were also detected. The oral live bacteria elicited obvious DTH reaction and proliferation response of peripheral lymphocytes by GZ-PCX antigen. The body weight of immunized mice slightly decreased but no other toxic effects was observed, which showed the safety of oral immunization. The study of oral live HCV multi-epitope vaccine might be able to provide a new route for the researches of HCV vaccines.
Huang
J
J
School of Life Science, Fudan University, Shanghai 200433.
Xie
Y
Y
Zhang
Q
Q
Shen
X
X
Ren
D
D
D000818:Animals; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005260:Female; D016174:Hepacivirus / Q000276:immunology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012486:Salmonella typhimurium / Q000235:genetics*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014761:Viral Hepatitis Vaccines / Q000276:immunology*; D001616:beta-Galactosidase / Q000235:genetics
D000939:Epitopes; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D014761:Viral Hepatitis Vaccines; D001616:beta-Galactosidase