title: The comprehensive mouse radiation hybrid map densely cross-referenced to the recombination map: a tool to support the sequence assemblies.
abstract: We have developed a unique comprehensive mouse radiation hybrid (RH) map of nearly 23,000 markers integrating data from three international genome centers and over 400 independent laboratories. We have cross-referenced this map to the 0.5-cM resolution recombination-based Jackson Laboratory (TJL) backcross panel map, building a complete set of RH framework chromosome maps based on a high density of known-ordered anchor markers. We have systematically typed markers to improve coverage and resolve discrepancies, and have reanalyzed data sets as needed. The cross-linking of the RH and recombination maps has resulted in a highly accurate genome-wide map with consistent marker order. We have compared these linked framework maps to the Ensemble mouse genome sequence assembly, and show that they are a useful medium resolution tool for both validating sequence assembly and elucidating chromosome biology.
authors.affiliation: The Jackson Laboratory, Bar Harbor, Maine 04609, USA. lbr@jax.org
authors.forename: Lucy B
authors.initials: LB
authors.lastname: Rowe
authors.forename: Mary E
authors.initials: ME
authors.lastname: Barter
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Kelmenson
authors.forename: Janan T
authors.initials: JT
authors.lastname: Eppig
mesh_terms: D000818:Animals; D000835:Animals, Wild; D002875:Chromosomes / Q000235:genetics; D020451:Contig Mapping; D006224:Cricetinae; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics; D023061:Gene Order / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D021101:Radiation Hybrid Mapping / Q000379:methods*; D011995:Recombination, Genetic / Q000235:genetics*; D014960:X Chromosome / Q000235:genetics
chemical_list: D005819:Genetic Markers; D004247:DNA