[Determination of triptolide in human plasma by gas chromatography-electron capture detection].
A method for the determination of tiptolide in human plasma by gas chromatography-electron capture detection is described. An SE-54 capillary column (30 m x 0.25 mm i.d.) was used. Nitrogen was used as the carrier gas. After NaOH solution being added, the plasma was extracted with ether-chloroform (3:1, V/V) and then reacted with trifluoroacetic anhydride. A good linearity was obtained from 1.0 microgram/L to 50.0 micrograms/L of tiptolide in human plasma, r = 0.9990. The detection limit of it in plasma was 0.5 microgram/L. The average recovery was 96.3%. The method is sensitive and precise.
Yang
L L
LL
Department of Clinical Pharmacology, Nanjing General Hospital of Nanjing Command PLA, Nanjing 210002, China. yllyj@elong.com
Zhang
X
X
Yuan
Y S
YS
D002849:Chromatography, Gas / Q000379:methods*; D004224:Diterpenes / Q000097:blood*; D019075:Electrophoresis, Capillary; D004852:Epoxy Compounds; D006801:Humans; D010616:Phenanthrenes / Q000097:blood*; D012680:Sensitivity and Specificity
D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; C001899:triptolide