[A study of polysaccharide-protein complex and glycosyls in the tissue surrounding titanium implant of rabbit].
OBJECTIVE
The absorption of the polysaccharide-protein complex to the implant surface may be the first reaction between the implant and the surrounding tissue. So we observed the distribution and the relative amount of the polysaccharide-protein complex and glycosyls in order to find out the possible functions and changing rules of these substances.


METHODS
12 rabbits were selected according to the age, sex, weight, and health conditions. Bilateral mandibular premolar teeth of rabbits were extracted under abdomen anesthesia (2% glutababituratic acid), then 12 titanium implants were placed into the teeth extraction holes of one side and left the other side to be the control one. All of the implants were required to be placed 2 mm below the alveolar crest, and tissue closure was achieved with mattress and interrupted single sutures. Antibiotic injections were given to the rabbits in the following 3 days. The animals were sacrificed at the end of 1 week, 4 weeks and 24 weeks. Tissue blocks (4 mm x 4 mm x 4 mm) containing the titanium implants and the control ones were removed, then fixed with 10% formaldehyde for 2 weeks, and decalcified with 10% EDTA (pH7.0) for 8 weeks. Carefully removed the titanium implants, the tissue blocks were embedded into the paraffin wax, then the sections were obtained and stained using following methods. 1. Alcian Blue Method: Alcian blue (pH 2.5) staining displayed the acidic proteoglycans; Alcian blue (pH 1.0) displayed the proteoglycans containing sulfonyl; Alcian blue/periodix acid-shiff staining demonstrated the acidic and neutral proteoglycans respectively. Alcian blue-MgCl2 with different ionic concentration identified the different kinds of proteoglycans. 2. Lectin Method: 5 kinds of lectins were used to locate 5 kinds of glycosyls. 2.5 micrograms/ml ConA located the glucose; 10 micrograms/ml PNA located the galactose; 10 micrograms/ml WGA located the N-acetylglucosamine; 25 micrograms/ml PHA-E located N-acetylgalactosamine, 10 micrograms/ml WPL loacted the fucose.


RESULTS
High level of proteoglycans and glycosyls were observed in the peri-implant connective tissue during all phases of the experiment. It was also observed that polysaccharide-protein complex and glycosyls reached a high level in new bone matrix as well as osteoblasts.


CONCLUSIONS
Polysaccharide-protein complex and glycosyls do play an important role in the implant-bone attachment, and titanium compatibility and may be closely related to the bone metabolic activity.
Bai
W
W
College of Stomatology, West China University of Medical Sciences.
Gong
P
P
Xiao
B
B
Xian
S
S
D000818:Animals; D015921:Dental Implants; D008297:Male; D010519:Periodontium / Q000378:metabolism* / Q000473:pathology; D011509:Proteoglycans / Q000378:metabolism*; D011817:Rabbits; D014025:Titanium
D015921:Dental Implants; D011509:Proteoglycans; D014025:Titanium