title: Immunohistochemical localization of the sigma1-receptor in oligodendrocytes in the rat central nervous system.
abstract: By using a new polyclonal antibody raised against a 21-amino acid peptide sequence corresponding to the fragment 138-157 of the cloned rat sigma(1)-receptor, we demonstrated by immunoperoxidase and double immunofluorescence techniques, that rat oligodendrocytes express the sigma(1)-receptor. Experiments in vivo and in vitro showed that sigma(1)-receptor colocalized with specific markers of progenitor (A2B5) and mature oligodendrocytes (GalC, RIP). These results suggest that sigma(1)-receptor in oligodendrocytes might be involved in myelination by direct implication in cholesterol biosynthesis or by interaction with endogenous ligands such as neurosteroids.
authors.affiliation: Histopathology Unit, Research Center, Laboratorios del Dr. Esteve, S.A., Av. Mare de Déu de Montserrat 221, 08041 Barcelona, Spain. gpalacios@esteve.es
authors.forename: Gabriel
authors.initials: G
authors.lastname: Palacios
authors.forename: Asunción
authors.initials: A
authors.lastname: Muro
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Vela
authors.forename: Eduardo
authors.initials: E
authors.lastname: Molina-Holgado
authors.forename: Xavier
authors.initials: X
authors.lastname: Guitart
authors.forename: Sergio
authors.initials: S
authors.lastname: Ovalle
authors.forename: Daniel
authors.initials: D
authors.lastname: Zamanillo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D005699:Galactosylceramides / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009836:Oligodendroglia / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017480:Receptors, sigma / Q000378:metabolism*; D014018:Tissue Distribution; D000097605:Sigma-1 Receptor
chemical_list: D000911:Antibodies, Monoclonal; D005699:Galactosylceramides; D011506:Proteins; D017480:Receptors, sigma; D000097605:Sigma-1 Receptor; C002454:galactocerebroside