[Cloning and expression of VHB gene in D-arabitol producing yeast].
Recombinant plasmid pVgb-EX2 containing Vitreoscilla hemoglobin gene vgb and formaldehyde resistant gene SFA1 was constructed and transformed into D-arabitol producing yeast strain Saccharomyces sp. X-62. The fact that the amount of VHb in transformant cells was considerably higher than that in control cells indicated that gene vgb was expressed in transformant cells. D-arabitol productivity and yield of fermentation by transformants were improved. The most improvement of D-arabitol productivity in repeat experiments reached 27.3%. It appeared that the fermentation productivity of D-arabitol was relative to the amount of VHb in cells under experimental conditions.
He
P
P
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Lu
D
D
Wang
Q
Q
Shen
A
A
Jiang
N
N
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D006454:Hemoglobins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D013402:Sugar Alcohols / Q000378:metabolism*; D014170:Transformation, Genetic; D020589:Vitreoscilla / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006454:Hemoglobins; D011994:Recombinant Proteins; D013402:Sugar Alcohols; C014999:arabitol