title: Transcriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis.
abstract: Paracoccidioides brasiliensis is a pathogenic fungus that undergoes a temperature-dependent cell morphology change from mycelium (22 degrees C) to yeast (36 degrees C). It is assumed that this morphological transition correlates with the infection of the human host. Our goal was to identify genes expressed in the mycelium (M) and yeast (Y) forms by EST sequencing in order to generate a partial map of the fungus transcriptome. Individual EST sequences were clustered by the CAP3 program and annotated using Blastx similarity analysis and InterPro Scan. Three different databases, GenBank nr, COG (clusters of orthologous groups) and GO (gene ontology) were used for annotation. A total of 3,938 (Y = 1,654 and M = 2,274) ESTs were sequenced and clustered into 597 contigs and 1,563 singlets, making up a total of 2,160 genes, which possibly represent one-quarter of the complete gene repertoire in P. brasiliensis. From this total, 1,040 were successfully annotated and 894 could be classified in 18 functional COG categories as follows: cellular metabolism (44%); information storage and processing (25%); cellular processes-cell division, posttranslational modifications, among others (19%); and genes of unknown functions (12%). Computer analysis enabled us to identify some genes potentially involved in the dimorphic transition and drug resistance. Furthermore, computer subtraction analysis revealed several genes possibly expressed in stage-specific forms of P. brasiliensis. Further analysis of these genes may provide new insights into the pathology and differentiation of P. brasiliensis.
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade de Brasília, 70910-900 Brasília, DF, Brasil. msueli@unb.br
authors.forename: M S S
authors.initials: MS
authors.lastname: Felipe
authors.forename: R V
authors.initials: RV
authors.lastname: Andrade
authors.forename: S S
authors.initials: SS
authors.lastname: Petrofeza
authors.forename: A Q
authors.initials: AQ
authors.lastname: Maranhão
authors.forename: F A G
authors.initials: FA
authors.lastname: Torres
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authors.lastname: Albuquerque
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authors.lastname: Azevedo
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authors.initials: LA
authors.lastname: Bataus
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authors.initials: CL
authors.lastname: Borges
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authors.initials: EG
authors.lastname: Campos
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authors.initials: MR
authors.lastname: Cruz
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authors.lastname: Daher
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authors.lastname: Dantas
authors.forename: M A S V
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authors.lastname: Ferreira
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authors.lastname: Ghil
authors.forename: R S A
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authors.lastname: Jesuino
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authors.lastname: Kyaw
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authors.lastname: Leitão
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authors.lastname: Martins
authors.forename: L M P
authors.initials: LM
authors.lastname: Moraes
authors.forename: E O
authors.initials: EO
authors.lastname: Neves
authors.forename: A M
authors.initials: AM
authors.lastname: Nicola
authors.forename: E S
authors.initials: ES
authors.lastname: Alves
authors.forename: J A
authors.initials: JA
authors.lastname: Parente
authors.forename: M
authors.initials: M
authors.lastname: Pereira
authors.forename: M J
authors.initials: MJ
authors.lastname: Poças-Fonseca
authors.forename: R
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authors.lastname: Resende
authors.forename: B M
authors.initials: BM
authors.lastname: Ribeiro
authors.forename: R R
authors.initials: RR
authors.lastname: Saldanha
authors.forename: S C
authors.initials: SC
authors.lastname: Santos
authors.forename: I
authors.initials: I
authors.lastname: Silva-Pereira
authors.forename: M A S
authors.initials: MA
authors.lastname: Silva
authors.forename: E
authors.initials: E
authors.lastname: Silveira
authors.forename: I C
authors.initials: IC
authors.lastname: Simões
authors.forename: R B A
authors.initials: RB
authors.lastname: Soares
authors.forename: D P
authors.initials: DP
authors.lastname: Souza
authors.forename: M T
authors.initials: MT
authors.lastname: De-Souza
authors.forename: E V
authors.initials: EV
authors.lastname: Andrade
authors.forename: M A S
authors.initials: MA
authors.lastname: Xavier
authors.forename: H P
authors.initials: HP
authors.lastname: Veiga
authors.forename: E J
authors.initials: EJ
authors.lastname: Venancio
authors.forename: M J A
authors.initials: MJ
authors.lastname: Carvalho
authors.forename: A G
authors.initials: AG
authors.lastname: Oliveira
authors.forename: M K
authors.initials: MK
authors.lastname: Inoue
authors.forename: N F
authors.initials: NF
authors.lastname: Almeida
authors.forename: M E M T
authors.initials: ME
authors.lastname: Walter
authors.forename: C M A
authors.initials: CM
authors.lastname: Soares
authors.forename: M M
authors.initials: MM
authors.lastname: Brígido
mesh_terms: D001483:Base Sequence; D001938:Brazil; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D016681:Genome, Fungal; D006801:Humans; D008969:Molecular Sequence Data; D010228:Paracoccidioides / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D004271:DNA, Fungal