title: Effect of matrine on cold ischemia and reperfusion injury of sinusoidal endothelial cells in rat orthotopic liver transplantation.
abstract: OBJECTIVE
  To study the mechanism and prevention of matrine (Mat) on cold ischemia/reperfusion injury of sinusoidal endothelial cells (SEC) in rat orthotopic liver transplantation (OLT).
  
  
  METHODS
  One hundred and twenty-six syngeneic SD rats were randomly divided into four groups (n=18): untreated group, 40 mg/kg treated group, 80 mg/kg treated group, and pseudo-treated group. After 5 h of preservation in Ringer's (LR) solution, orthotopic implantation of the donor liver was performed. At 1, 2, and 4 h after reperfusion of the portal vein, 6 rats were killed in each group to collect the serum and the median lobe of liver for assay.
  
  
  RESULTS
  The level of hylluronic acid (HA) and intercellular adhesion molecule-1 (ICAM-1) decreased significantly in both treated groups at different times post-transplantation, and their pathological changes of SEC were ameliorated, too.
  
  
  CONCLUSIONS
  Matrine can prevent SEC from ischemia and reperfusion injury in rat orthotopic liver transplantation.
authors.affiliation: Department of Hepatobiliary Surgery, Drum Tower Hospital, Medical Department of Nanjing University, 210008, China. drzhu@elong.com
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Zhu
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Qiu
authors.forename: Ming-Ke
authors.initials: MK
authors.lastname: Shi
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.forename: Xiao-Gang
authors.initials: XG
authors.lastname: Zheng
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Ding
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D003080:Cold Temperature; D042783:Endothelial Cells / Q000378:metabolism; D006820:Hyaluronic Acid / Q000097:blood; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D007511:Ischemia; D008099:Liver / Q000378:metabolism / Q000473:pathology; D016031:Liver Transplantation; D008297:Male; D020011:Protective Agents / Q000494:pharmacology; D011807:Quinolizines; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D020011:Protective Agents; D011807:Quinolizines; D018799:Intercellular Adhesion Molecule-1; D006820:Hyaluronic Acid; D000093842:Matrines