title: Block in development at the pre-B-II to immature B cell stage in mice without Ig kappa and Ig lambda light chain.
abstract: Silencing individual C (constant region) lambda genes in a kappa(-/-) background reduces mature B cell levels, and L chain-deficient (lambda(-/-)kappa(-/-)) mice attain a complete block in B cell development at the stage when L chain rearrangement, resulting in surface IgM expression, should be completed. L chain deficiency prevents B cell receptor association, and L chain function cannot be substituted (e.g., by surrogate L chain). Nevertheless, precursor cell levels, controlled by developmental progression and checkpoint apoptosis, are maintained, and B cell development in the bone marrow is fully retained up to the immature stage. L chain deficiency allows H chain retention in the cytoplasm, but prevents H chain release from the cell, and as a result secondary lymphoid organs are B cell depleted while T cell levels remain normal.
authors.affiliation: Laboratory of Developmental Immunology, The Babraham Institute, Babraham, Cambridge, United Kingdom.
authors.forename: Xiangang
authors.initials: X
authors.lastname: Zou
authors.forename: Tony A
authors.initials: TA
authors.lastname: Piper
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Smith
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Allen
authors.forename: Jian
authors.initials: J
authors.lastname: Xian
authors.forename: Marianne
authors.initials: M
authors.lastname: Brüggemann
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000473:pathology; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D003593:Cytoplasm / Q000235:genetics / Q000276:immunology; D017353:Gene Deletion; D020868:Gene Silencing / Q000276:immunology; D005819:Genetic Markers / Q000276:immunology; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D007146:Immunoglobulin lambda-Chains / Q000235:genetics*; D007148:Immunoglobulin mu-Chains / Q000235:genetics; D008231:Lymphopenia / Q000235:genetics / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D010446:Peptide Fragments / Q000235:genetics; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology* / Q000473:pathology
chemical_list: D005819:Genetic Markers; D007127:Immunoglobulin Constant Regions; D007143:Immunoglobulin Heavy Chains; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D007148:Immunoglobulin mu-Chains; D010446:Peptide Fragments