title: [Study on proteins of allele by using the microisoelectric focusing].
abstract: Clear protein electrophoresis pattern of single pollen grain from Cucurbita pepo L. was obtained with isoelectric focusing and supersensitive stain technique. The results showed that there were two special distributing protein bands near acidity pole. In species I, the two protein bands were a little near to acidity pole and in species II, that was a little far from acidity pole. In species III, the two protein bands distributed either like species I or like species II and the distributed ratio was 1:1. Moreover, we discuss how to identify relative purity of gene with the sorts of the special proteins.
authors.affiliation: Department of Biology, Henan Normal University, Xinxiang 453002, China.
authors.forename: D
authors.initials: D
authors.lastname: Zhao
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: G
authors.initials: G
authors.lastname: Pang
authors.forename: Z
authors.initials: Z
authors.lastname: Deng
authors.forename: Q
authors.initials: Q
authors.lastname: Shao
authors.forename: X
authors.initials: X
authors.lastname: Yang
mesh_terms: D000483:Alleles; D028464:Cucurbita / Q000737:chemistry* / Q000235:genetics; D004586:Electrophoresis / Q000379:methods; D015870:Gene Expression; D007525:Isoelectric Focusing / Q000379:methods*; D014674:Plant Proteins, Dietary / Q000235:genetics*; D011058:Pollen / Q000737:chemistry* / Q000235:genetics
chemical_list: D014674:Plant Proteins, Dietary