[Significance of programmed cell death in C57BL/6N strain mouse palate process development and cleft palate formation].
OBJECTIVE
To observe the changes of programmed cell death (PCD) in C57BL/6N strain mouse cleft palate formation and study related gene of PCD in future.


METHODS
16 mice (gestation of 10 days, GD10) were divided into the experimental and control groups randomly, then the mice of the experimental group were dosed with retinoic acid(RA) 80 mg/kg, and the tissue slices of the embryo mouse were prepared for TdT-mediated dUTP nick end labeling (TUNEL) staining at GD13(14)(the 14th hour of the 13th day gestation), GD13(22), GD14(8), GD14(14), GD14(22), GD15(8), GD15(22) and GD16(8) respectively.


RESULTS
In the early development of palate process, the positive index of PCD was significantly higher in the experimental group than in the control group (P < 0.05).


CONCLUSIONS
The effects of RA on mesenchymal cells of the early development of palate process in the experimental group induce PCD, which result in the formation of small palate process and failure of fusing of the shelves.
Li
X
X
Department of Oral Pathology, Stomatology College, Fourth Military Medical University.
Jin
Y
Y
Dong
S
S
Zhu
X
X
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002972:Cleft Palate / Q000139:chemically induced* / Q000196:embryology; D004622:Embryo, Mammalian / Q000187:drug effects; D005260:Female; D020287:In Situ Nick-End Labeling; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010159:Palate / Q000196:embryology*; D011247:Pregnancy; D014212:Tretinoin / Q000633:toxicity
D014212:Tretinoin