[Expression of xylose isomerase gene(xylA) in Saccharomyces cerevisiae from Clostridium thermohydrosulfuricum].
The Clostridium thermohydrosulfuricum xylA gene encoding xylose(glucose) isomerase was cloned in the yeast expression vector pMA91 under the control of the PGK promoter, resulting in pBX-1, and transformed into Saccharomyces cerevisiae. Production of recombinant xylose isomerase was seen in the a Coomassie stained SDS-PAGE gel and the molecular mass was estimated to be 43 kD. The recombinant xylose isomerase showed the highest activity at 85 degrees C and pH7. The specific activity under these condition was 1.0 U/mg protein. At 30 degrees C and 40 degrees C, the relative activity was reduced to 3.7% and 11%, respectively, of the maximum.
Bao
X
X
State Key Lab of Microbial Technology, Shandong University, Jinan 250100.
Gao
D
D
Wang
Z
Z
D019747:Aldose-Ketose Isomerases / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D003013:Clostridium / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
D011994:Recombinant Proteins; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase