[Rapid determination of metallothioneins in foods by capillary zone electrophoresis].
A rapid method for the analysis of metallothioneins (MT) in foods by capillary zone electrophoresis(CZE) was developed. Two isomers of MT (MT1, MT2) in foods were separated and determined. After a series of optimization, the separation and determination of MT1 and MT2 were obtained within 10 min by using the phosphate buffer system consisting of 0.02 mol/L Na2HPO4 and 0.02 mol/L NaH2PO4 (pH 7.0), and UV detection at 200 nm. Under the optimal experimental conditions, the minimum detectable limit was 1 mg/L, and the added standard recoveries of MT1, MT2 in foods were found to be in the range of 82.0%-93.4%. The relative standard deviations (RSD) were found to be lower than 10%. Therefore, with this simple and rapid method, the contents of two isomers in foods can be determined by external standard method after sample pretreatment.
Hao
Shou-Jin
SJ
National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, Beijing 100871, China. haoshouj@hotmail.com
Li
Xuan
X
Kang
Jun-Xing
JX
Zhao
Rong
R
Zhao
Shan
S
Tang
Qing-Ping
QP
Ru
Bing-Gen
BG
D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D005399:Fishes; D005504:Food Analysis; D008668:Metallothionein / Q000032:analysis* / Q000302:isolation & purification; D008892:Milk / Q000737:chemistry; D011817:Rabbits; D051381:Rats; D013237:Stereoisomerism
D008668:Metallothionein