[Distribution of peptidergic neurons in the rat suprachiasmatic nucleus].
The distribution of the peptidergic neurons in the suprachiasmatic nucleus(SCN) in five adult male SD rats was examined by multi-immunolabelling for vasoactive intestinal polypeptide(VIP), arginine vasopressin (AVP) and somatostatin(SOM). The immunoreactions were visualized with an Elite ABC Kit(Vector Lab). The substrates in the peroxidase reaction were diaminobenzidine(DAB), tetramethylbenzidine(TMB) and 4-chloro-1-naphtol. The results showed that the differences in distribution and population of VIP-, AVP- and SOM-like neurons existed in the rostral, intermediate and caudal regions of the SCN and these neurons were overlapped and SOM-like neurons were scattered among them in the area between VIP- and AVP-like neurons. These findings may provide new morphologic evidences for explaining the functional mechanism in the SCN.
Wang
L
L
Department of Histology and Embryology, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
Ou
K
K
Cao
G
G
Chen
W
W
D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis*; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013004:Somatostatin / Q000032:analysis*; D013493:Suprachiasmatic Nucleus / Q000737:chemistry*; D014660:Vasoactive Intestinal Peptide / Q000032:analysis*
D001127:Arginine Vasopressin; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin