Characterization of phosphodiesterase 3 in human malignant melanoma cell line.
Little is known concerning the expression, distribution and function of phosphodiesterase (PDE) 3s in malignant tumor cells, including human malignant melanoma HMG and osteosarcoma HOSM-1 cells. PDE3 activity was detected in homogenates of HMG cells; however, much less activity was found in HOSM-1 cells. In HMG cells, most of the PDE3 activity was in the particulate fraction. PDE3A and 3B mRNAs were detected by RT-PCR in RNA from HMG cells only. The nucleotide sequences of the fragments were identical to those of human PDE3A and 3B. The PDE3-specific inhibitors, trequinsin and cilostamide, did not inhibit the proliferation of HMG or HOSM-1 cells. Although two PDE3 isoforms may be expressed in human malignant melanoma cells, their functional importance is not known.
Murata
Taku
T
Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Mie University, 2-174, Edobashi, Tsu, Mie 514-8507, Japan. muratat@clin.medic.mie-u.ac.jp
Shimizu
Kasumi
K
Narita
Motoshi
M
Manganiello
Vincent C
VC
Tagawa
Toshiro
T
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D001859:Bone Neoplasms / Q000201:enzymology; D002455:Cell Division / Q000502:physiology; D000242:Cyclic AMP / Q000378:metabolism; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D007546:Isoquinolines / Q000494:pharmacology; D008545:Melanoma / Q000201:enzymology*; D012516:Osteosarcoma / Q000201:enzymology; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D015363:Quinolones / Q000494:pharmacology; D044005:Tetrahydroisoquinolines; D014316:Tritium; D014407:Tumor Cells, Cultured
D007527:Isoenzymes; D007546:Isoquinolines; D010726:Phosphodiesterase Inhibitors; D015363:Quinolones; D044005:Tetrahydroisoquinolines; D014316:Tritium; C021294:cilostamide; C035964:trequinsin; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; C517287:PDE3A protein, human