[Isolation and characterization of a insecticidal protein from Pseudomonas pseudoalcaligenes].
A insecticidal protein was purified by gel-filtration chromatography on Sephadex G-100 and anion-exchange chromatography on DEAE-Sephadex A-50 from the suspension of Pseudomonas pseudoalcaligenes culture. Certain biophysical and biochemical properties were also studied. The molecular weight of the subunit of the insecticidal protein is 25,100 and pI is 5.16. Amino acid composition analysis showed that it is an acidic protein.
Zhang
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W
Natoinal Laboratory for Grassland Biological Control of Sichuan University, Chengdu 610064.
Yang
Z
Z
Zhu
W
W
Hou
T
T
Liu
S
S
D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D011549:Pseudomonas / Q000737:chemistry*
D000596:Amino Acids; D001426:Bacterial Proteins