Continuous production of a peptidic fraction containing the intermediate opioid peptide LVV-haemorphin-7 (LVVh-7) by peptic hydrolysis of bovine haemoglobin in a continuous membrane reactor.
Peptic hydrolysis of native bovine haemoglobin at pH 3 yields the LVV-haemorphin-7 (Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe; LVVh-7) opioid peptide corresponding to the residues-31-40 fragment of the beta-chain of haemoglobin. This peptide is intermediate in the course of batch hydrolysis and is rapidly degraded. Indeed, it shows an optimum at 3% degree of hydrolysis (i.e. 2 min of reaction time). The hydrolysis was carried out in a continuous membrane reactor with a space time (ratio of the flux to the reactor volume) set to 2 min (corresponding to optimum LVVh-7 production). This process allows the continuous production of a constant fraction of intermediate peptides containing LVVh-7 for 48 min.
Kapel
Romain
R
Laboratoire de Technologie des Substances Naturelles, Bâtiment EUDIL/IAAL aile C, boulevard Paul Langevin, cité scientifique, 59655 Villeneuve d'Ascq, France.
Froidevaux
Renato
R
Nedjar-Arroume
Naima
N
Fertin-Bazus
Anne
A
Dhulster
Pascal
P
Guillochon
Didier
D
D000818:Animals; D019149:Bioreactors; D002417:Cattle; D006454:Hemoglobins / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification*; D006868:Hydrolysis; D008567:Membranes, Artificial; D018847:Opioid Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000302:isolation & purification*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D014462:Ultrafiltration / Q000379:methods*
D006454:Hemoglobins; D008567:Membranes, Artificial; D018847:Opioid Peptides; D010446:Peptide Fragments; D010455:Peptides; C074309:LVV-hemorphin-7