title: [Determination of triptolide in human plasma by gas chromatography-electron capture detection].
abstract: A method for the determination of tiptolide in human plasma by gas chromatography-electron capture detection is described. An SE-54 capillary column (30 m x 0.25 mm i.d.) was used. Nitrogen was used as the carrier gas. After NaOH solution being added, the plasma was extracted with ether-chloroform (3:1, V/V) and then reacted with trifluoroacetic anhydride. A good linearity was obtained from 1.0 microgram/L to 50.0 micrograms/L of tiptolide in human plasma, r = 0.9990. The detection limit of it in plasma was 0.5 microgram/L. The average recovery was 96.3%. The method is sensitive and precise.
authors.affiliation: Department of Clinical Pharmacology, Nanjing General Hospital of Nanjing Command PLA, Nanjing 210002, China. yllyj@elong.com
authors.forename: L L
authors.initials: LL
authors.lastname: Yang
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: Y S
authors.initials: YS
authors.lastname: Yuan
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D004224:Diterpenes / Q000097:blood*; D019075:Electrophoresis, Capillary; D004852:Epoxy Compounds; D006801:Humans; D010616:Phenanthrenes / Q000097:blood*; D012680:Sensitivity and Specificity
chemical_list: D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; C001899:triptolide