Synthesis of osteogenic growth peptide and its synergetic effect with granulocyte colony stimulating factor.
Osteogenic growth peptide (OGP) has been synthesized through Fmoc solid phase synthesis procedure. The purity of synthetic OGP (sOGP) is over 98.6% identified by HPLC, the amino acid sequence and electro-spray mass spectroscopy are consistent with theoretical values. The synergetic effect of sOGP with recombinant human granulocyte-colony stimulating factor (rhG-CSF) on the hematopoiesis was investigated in normal mice. To assess the synergy of sOGP with rhG-CSF, two schemes were designed. In one scheme rhG-CSF was used at the last 8 days of a 13-day treatment with sOGP, while in the other one both cytokines were given concurrently for 10 days [sOGP, 0.5 nmol/day (mouse); rhG-CSF, 2 microg/day (mouse)]. Both schemes showed that sOGP remarkably synergized with rhG-CSF on increment of white blood cell number and lymphocyte number in peripheral blood without any change of red blood cell and platelet counts. Quantitative differential analysis of bone marrow and histological examination of the spleen and sternum showed that sOGP plus rhG-CSF did not cause abnormal hyperplasia, so sOGP is a very hopeful new drug to improve the effectiveness of clinical used rhG-CSF.
Liu
Zhi-Hui
ZH
Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China.
Shao
Yun-Chao
YC
Chen
Hong-Hong
HH
Shi
De-Yuan
DY
Chen
Tong-Yi
TY
Cui
Da-Fu
DF
D000818:Animals; D001772:Blood Cell Count; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004357:Drug Synergism; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006133:Growth Substances / Q000138:chemical synthesis / Q000494:pharmacology*; D006410:Hematopoiesis / Q000187:drug effects; D006657:Histones; D036341:Intercellular Signaling Peptides and Proteins / Q000138:chemical synthesis / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D006133:Growth Substances; D006657:Histones; D036341:Intercellular Signaling Peptides and Proteins; D011994:Recombinant Proteins; C074533:osteogenic growth peptide; D016179:Granulocyte Colony-Stimulating Factor