[Determination of 10-HDA in honeybee body by HPLC].
In the present work we found that in the honeybee body there exists an unsaturated fatty acid, trans-10-hydroxy-2-decenoic acid (10-HDA), which was known only to be present in royal jelly. We established the analytical method of 10-HDA in honeybee body by HPLC and simplified the extraction method of 10-HDA. In the optimum conditions the linear range of detection was 10-1,000 ng, the correlation coefficient was 0.9998, the recovery was 96.5%-99.2% and the detectable limit was 0.53 microgram/g.
Fan
H
H
Central Laboratory, Shandong Agricultural University, Taian, 271018.
He
C
C
Han
H
H
D000818:Animals; D001516:Bees / Q000033:anatomy & histology / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005229:Fatty Acids, Monounsaturated / Q000032:analysis* / Q000302:isolation & purification; D000432:Methanol / Q000737:chemistry; D015203:Reproducibility of Results
D005229:Fatty Acids, Monounsaturated; C055543:10-hydroxy-2-decenoic acid; D000432:Methanol