[Delivery of secreted placental alkaline phosphatase (SEAP) gene in vitro and in vivo as a component of recombinant avian adenovirus (CELO)].
Recombinant adenoviruses capable of expressing the gene of secreted placentary alkaline phosphatase (SEAP) under control of CMV-promoter was obtained on the basis of CELO avian adenovirus and human adenovirus-5 (Ad5) genomes. The efficiency of the CELO vector was determined in experiments with transduction of human (293, A549, and H1299), mouse (B16), and avian (LMH) cell cultures. It was shown in C57BL/6 mice in vivo that SEAP gene is expressed under conditions of intravenous, intranasal, and intratumoral application of recombinant adenovirus CELO-SEAP. The duration of expression of the alkaline phosphatase CELO = SEAP gene in immunocompetent mouse body was 21 days. The level of SEAP gene expression was measured in the allantois fluid of chicken embryo infected with recombinant adenovirus CELO-SEAP.
Logunov
D Iu
DIu
All-Russian Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences, Moscow.
Cherenova
L V
LV
Shmarov
M M
MM
Shashkova
E V
EV
Verkhovskaia
L V
LV
Doronin
K K
KK
Naroditskiĭ
B S
BS
D000469:Alkaline Phosphatase / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D000482:Allantois / Q000201:enzymology / Q000378:metabolism / Q000821:virology; D000818:Animals; D002478:Cells, Cultured / Q000821:virology; D002642:Chick Embryo; D005260:Female; D025681:Fowl adenovirus A / Q000235:genetics* / Q000472:pathogenicity; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics; D016679:Genome, Viral; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D014161:Transduction, Genetic
D011994:Recombinant Proteins; D000469:Alkaline Phosphatase