[A Carnobacterium-like organism isolated form a patient with multiple bacterial synergistic gangrene].
An atypical lactic-acid producing gram positive rod Y6 strain was studied in this report, which was isolated form clinic sample of a patient with multiple bacterial synergistic gangrene, and could not be identified by routine method. A 1.5 kb of 16S rDNA of Y6 strain was synthesized and sequenced. Comparative 16S rDNA sequence analyses revealed that stain Y6 is most closely related to the genus of Carnobacterium. The overall similarity value between Y6 strain and Carnobacterium species are 93% to 97%. The signature nucleotides in 16S rDNA primary sequence of strain Y6 and that of genus of Carnobacterium were identical. The biological features of Y6 strain are very similar to that of Carnobacterium, such as lactic acid as main end product of in PYG broth, no gas produced form fermentation of glucose, catalase negative, no motility. Data suggested that Y6 strain is very similar to the genus of Carnobacterium, of which no strain has been isolated form clinical sample so far. Based on the results obtained, we names Y6 strain as Carnobacterium-like organism.
Xu
J
J
Laboratory of Molecular Medical Bacteriology, Ministry of Health, Institute of Epidemiology and Microbiology, Chinese Academy for Preventive Medicine, Beijing 102206.
Yang
H
H
Wu
J
J
Lai
X
X
Liu
B
B
D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal; D005734:Gangrene / Q000382:microbiology*; D016987:Gram-Positive Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial