[Enantiomeric separation of N-FMOC amino acids with sulfobutyl ether-beta-cyclodextrin as chiral additive by capillary electrokinetic chromatography].
Enantiomeric separation of N-FMOC amino acids by capillary electrophoresis with an anionic chiral resolving agent, sulfobutyl ether-beta-cyclodextrin (SBE-beta-CD) with average substitution degree of 3.8, employed as chiral additive was studied. Chiral separation was achieved with the mode of electrokinetic chromatography due to the electrophoretic mobility and chiral complexing ability of the chiral additive. The effects of pH of background electrolyte, the concentration of sulfobutyl ether-beta-cyclodextrin on enantiomeric separations were investigated. The enantiomeric separations of 8 N-FMOC amino acids were accomplished, among which 5 N-FMOC amino acids could be on baseline separated.
Ruan
Z Q
ZQ
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
Li
J B
JB
Lu
H J
HJ
Ou
Q Y
QY
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003505:Cyclodextrins; D005449:Fluorenes / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D000596:Amino Acids; D003505:Cyclodextrins; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D047392:beta-Cyclodextrins; C093196:SBE4-beta-cyclodextrin