title: Breakdown of the blood-brain barrier and neuropathological changes induced by Phoneutria nigriventer spider venom.
abstract: The blood-brain barrier (BBB) is responsible for selective flux of substances between blood and brain. The selective permeability of the BBB is crucial for the maintenance of the brain microenvironment homeostasis, and alterations in the barrier may be involved in many pathophysiological processes. Phoneutria nigriventer armed spider venom produces excitatory signals and symptoms in humans, and its recognized neurotoxic action suggests a potential ability to alter BBB permeability. The aim of the present study was to investigate the capacity of P. nigriventer venom (PNV) in promoting BBB breakdown in adult rats. After intravenous injection of 850 micro g/kg of the whole venom, BBB lesions were evaluated after 18 h to 9 days by ultrastructural methods using the extracellular tracer lanthanum nitrate. Clinical signs and symptoms of rats showed acute neurotoxicity, with some of the animals presenting convulsions, but which were clinically resolved by 12 h post-envenoming. The results showed that PNV is able to increase BBB permeability, particularly in the hippocampus. Changes were first detected in arterioles and post-capillary venules 18 h to 5 days after venom inoculation. The increased permeation of the extracellular tracer peaked on day 1, representing about 42% of the examined vessels (P<0.01). This appeared to occur by both transendothelial and intercellular routes, i.e., by pinocytic transport and through interendothelial junctions. Concomitantly, the surrounding tissue showed vasogenic edema and swollen astrocytic processes, without inflammatory infiltrates. The peak of the edema occurrence was observed on day 3, in about 60% of the vessels (P<0.001). Enhanced capillary permeability was observed on day 9, and affected 36% of all capillaries (P<0.05). The affected capillaries were characterized by increased number of pinocytotic vesicles, which, in addition, were filled with the extracellular tracer, but without visible transport through the interendothelial pathway. This study demonstrates that systemic PNV inoculation induces BBB breakdown through trans- and paracellular routes. It is concluded that BBB breakdown is an event not associated with the acute neurotoxicity exhibited by the rats.
authors.affiliation: Departamento de Histologia e Embriologia, Instituto de Biologia, Caixa Postal 6109, Universidade Estadual de Campinas (UNICAMP), S.P., CEP 13083-970, Brasil.
authors.forename: Luciana
authors.initials: L
authors.lastname: de Paula Le Sueur
authors.forename: Evanguedes
authors.initials: E
authors.lastname: Kalapothakis
authors.forename: Maria Alice
authors.initials: MA
authors.lastname: da Cruz-Höfling
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects*; D001921:Brain / Q000098:blood supply* / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology / Q000648:ultrastructure; D001929:Brain Edema / Q000139:chemically induced; D002199:Capillary Permeability / Q000187:drug effects*; D005110:Extracellular Space; D008297:Male; D008854:Microscopy, Electron; D020258:Neurotoxicity Syndromes / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D013111:Spider Venoms / Q000493:pharmacokinetics*
chemical_list: D013111:Spider Venoms