Construction of artificial virus-like particles exposing HIV epitopes, and the study of their immunogenic properties.
One of the major problems in the development of successful recombinant vaccines against human immunodeficiency virus (HIV) is that of correct identification of a safe and effective vaccine delivery system with which to induce protective immunity using soluble protein antigens. An original method for constructing artificial immunogens in the form of spherical particles with yeast dsRNA in the center and hybrid proteins exposing epitopes of an infectious agent on the surface is reported. The dsRNA and the proteins were linked with spermidine-polyglucin-glutathione conjugates. Particles exposing HIV-1 epitopes were constructed, and their immunogenicity tested.
Karpenko
Larisa I
LI
The State Research Center of Virology and Biotechnology Vector, The Joint-Stock Company Vector Best 630559, Novosibirsk Region, Koltsovo, Russia. karpenko@vector.nsc.ru
Lebedev
Leonid R
LR
Ignatyev
George M
GM
Agafonov
Alexander P
AP
Poryvaeva
Vera A
VA
Pronyaeva
Tatiana R
TR
Ryabchikova
Elena I
EI
Pokrovsky
Andrei G
AG
Ilyichev
Alexander A
AA
D016915:AIDS Vaccines / Q000138:chemical synthesis / Q000276:immunology*; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D005822:Genetic Vectors; D015497:HIV-1 / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009500:Neutralization Tests; D011134:Polysaccharides / Q000276:immunology; D012330:RNA, Double-Stranded / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013601:T-Lymphocytes / Q000276:immunology
D016915:AIDS Vaccines; D000939:Epitopes; D011134:Polysaccharides; D012330:RNA, Double-Stranded; D011994:Recombinant Proteins