[Expression and insecticidal characteristics of cry3A in Lepidoptera-specific Bacillus thuringiensis].
The cry3A gene coding Coleoptera-specific protein was transformed into a wild strain YBT803-1 by electroporation, A transformant BMBY-001 was obtained. SDS-PAGE analysis demonstrated that cry3A could express 64 kD protein in BMBY-001. However, the expression of cry1Ab and cry1Ac were effected. Bioassay showed that BMBY-001 was not only highly toxic to Phyllodecta vulgatissima larva(LC50 0.413 microL/mL) but also toxic to Plutella xylostella(LC50 3.319 microL/mL).
Yue
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C
Department of Microbial Science and Technology, Huazhong Agricultural University, Wuhan 430070.
Liu
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Zeng
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X
Shao
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Z
Yu
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Z
D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity*; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity*; D001517:Coleoptera / Q000187:drug effects; D004731:Endotoxins / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity*; D015870:Gene Expression; D006460:Hemolysin Proteins; D007814:Larva / Q000187:drug effects; D007915:Lepidoptera / Q000187:drug effects / Q000382:microbiology*; D014170:Transformation, Genetic
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis