Over expression of bone morphogenetic protein-3b (BMP-3b) using an adenoviral vector promote the osteoblastic differentiation in C2C12 cells and augment the bone formation induced by bone morphogenetic protein-2 (BMP-2) in rats.
BMP-3b is a novel BMP-3-related protein and its biological functions are unknown. In order to investigate the biological actions of BMP-3b, we constructed a BMP-3b-expressing recombinant adenoviral vector (AxCAKBMP-3b). We show that over expression of BMP-3b stimulated the induction of differentiation and the osteoinduction activity of a human BMP-2-expressing recombinant adenoviral vector (AxCAOBMP-2). C2C12 cells were infected in vitro with AxCAKBMP-3b, AxCAOBMP-2 and a control vector containing no foreign genes (AxCAwt). Cells infected with AxCAOBMP-2 and AxCAKBMP-3b produced more alkaline phosphatase and secreted more osteocalcin into the culture medium than cells infected with AxCAOBMP-2 and AxCAwt. When AxCAOBMP-2, AxCAKBMP-3b, and AxCAwt were injected into the calf muscles of nude rats (F 344/N Jcl-rnu), the osteoinduction seen with AxCAOBMP-2 and AxCAKBMP-3b was greater than that seen with AxCAOBMP-2 and AxCAwt.
Kaihara
Shinji
S
Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo, Kyoto 606-8507, Japan.
Bessho
Kazuhisa
K
Okubo
Yasunori
Y
Sonobe
Junya
J
Komatsu
Yasato
Y
Miura
Masako
M
Miyatake
Shin-Ichi
S
Nakao
Kazuwa
K
Iizuka
Tadahiko
T
D000256:Adenoviridae / Q000235:genetics*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D001857:Bone Matrix / Q000033:anatomy & histology / Q000254:growth & development / Q000502:physiology; D055396:Bone Morphogenetic Protein 2; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects*; D005822:Genetic Vectors / Q000235:genetics*; D055413:Growth Differentiation Factor 10; D006801:Humans; D008297:Male; D010006:Osteoblasts / Q000187:drug effects*; D015675:Osteocalcin / Q000096:biosynthesis; D051381:Rats; D011916:Rats, Inbred F344; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta
C525718:BMP2 protein, human; C525757:BMP3 protein, human; C525720:Bmp2 protein, rat; C525759:Bmp3 protein, rat; D055396:Bone Morphogenetic Protein 2; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins; C525785:GDF10 protein, human; C525786:Gdf10 protein, rat; D055413:Growth Differentiation Factor 10; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D015675:Osteocalcin; D000469:Alkaline Phosphatase; D002118:Calcium