Reciprocal action between BMP-2 and BMP-3 in cultured fibroblast in vitro.
OBJECTIVE
To explore reciprocal action between BMP-2 (bone morphogenetic protein-2) and BMP-3 for better understanding of the mechanism of BMP during bone fracture union.


METHODS
rhBMP-2 was added into the cultured fibroblasts with the concentration of 1,200 ng/ml. The expression of BMP-3 in fibroblasts was detected by immunohistochemistry. Eukaryotic expression vector pcDNA3-BMP-3 was transfected into the fibroblasts. After the effective expression of BMP-3 was identified, BMP-2 was also detected by immunohistochemistry in BMP-3 expression cells. The fibroblasts transfected with empty vector pcDNA3 were used as the control.


RESULTS
Exogenous rhBMP-2 could promote the expression of BMP-3 in fibroblasts. BMP-3 also could be detected in these cells.


CONCLUSIONS
BMP-2 and BMP-3 could reciprocally adjust the expression in fibroblasts.
Meng
Guo-lin
GL
Orthopaedic Institute of PLA, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. mengguolin@163.net
Hu
Yun-yu
YY
Pu
Qin
Q
Lu
Rong
R
Yang
Liu
L
Wang
Jun
J
Bai
Jian-ping
JP
Xu
Xin-zhi
XZ
Li
Dan
D
D055396:Bone Morphogenetic Protein 2; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D017102:Fracture Healing / Q000502:physiology*; D007150:Immunohistochemistry; D010012:Osteogenesis / Q000502:physiology*; D016212:Transforming Growth Factor beta
D055396:Bone Morphogenetic Protein 2; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins; D016212:Transforming Growth Factor beta