[Determination of total sulfite in wine by high performance liquid chromatography(HPLC) with fluorescence detection].
A highly sensitive fluorometric method for the determination of total sulfite in wine is reported. The strong fluorescent derivatives formed by the quantitative reaction of sulfite in wine and N-(9-acridinyl) maleimide(NAM) in aqueous solution under mild conditions was injected into the HPLC. Separation was performed on an ODS column(200 mm x 5 mm) with a mobile phase of V(CH3CN):V(0.1 mo/L NH4AC) = 25:75 at a flow rate of 1.0 mL/min and detected by fluorometry at 455 nm(excitation at 365 nm). There was a good linear calibration curve in the range from 0.25-4 mg/L(r = 0.9998), The limit of detection was 1 ng. Sulfite mass concentration in wine has been determined by this method with good results and recoveries. This method is simple, rapid and reliable, and a sample size of only 1 mL is required to complete an analysis.
Wei
F
F
Dalian Import Food Hygiene Supervision and Inspection Center of P.R.C., Dalian 116001, China.
Chen
M S
MS
Yu
L
L
Chang
L
L
Li
D M
DM
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008301:Maleimides / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013447:Sulfites / Q000032:analysis*; D014920:Wine / Q000032:analysis*
D008301:Maleimides; D013447:Sulfites; C080490:N-(9-acridinyl)maleimide