title: Peripheral mononuclear cell IL-10 and IL-12 production is not impaired in patients with advanced cancer and severe infection.
abstract: We assessed whether interleukin (IL)-10 and IL-12 production of blood mononuclear cells (PBMC) after lipopolysaccharide (LPS 0.1 microgram/ml, 1.0 microgram/ml) or phytohemagglutinin (PHA 5 microgram/ml) stimulation in advanced cancer patients with severe infections (IA, n=10) differs from those without infection (AC,n =10) or healthy controls HC (n=10). PBMCs in IA produced statistically higher median IL-12 levels than those in HC; LPS 0.1 (P=0.05), LPS 1.0 (P=0.019) and PHA (P=0.024). IL-10 production was always higher in AC than in HC. Both the IL-10 (LPS 1.0; P=0.018) and IL-12 (LPS 0.1;P =0.046) production of PBMCs were significantly higher in IA than one month afterwards. In conclusion, the PBMC production of IL-10 and IL-12 in advanced cancer patients with or without infection was not impaired but rather enhanced compared to that of HC. One month after infection, however, PBMC production clearly decreased, suggesting a kind of hyporeactivity.
authors.affiliation: Department of Oncology and Radiotherapy, Oulu University Hospital, Oulu, Finland. raija.kallio@ppshp.fi
authors.forename: Raija
authors.initials: R
authors.lastname: Kallio
authors.forename: Heljä Marja
authors.initials: HM
authors.lastname: Surcel
authors.forename: Hannu
authors.initials: H
authors.lastname: Syrjälä
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007239:Infections / Q000378:metabolism*; D016753:Interleukin-10 / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D018664:Interleukin-12 / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000378:metabolism*
chemical_list: D016753:Interleukin-10; D018664:Interleukin-12