title: Effects of colony-stimulating factors on the secretion of platelet-activating factor acetylhydrolase by human decidual macrophages.
abstract: OBJECTIVE
  The aim is to clarify the role of platelet-activating factor and colony-stimulating factors in term and preterm parturition.
  
  
  METHODS
  Decidual macrophage populations were obtained by an enzymic digestion, Ficoll-Paque centrifugation, or flow cytometric sorting. The effects of colony-stimulating factors on the platelet-activating factor acetylhydrolase secretion by these cells were examined.
  
  
  RESULTS
  Granulocyte-macrophage colony-stimulating factor inhibited the platelet-activating factor acetylhydrolase secretion by decidual macrophages. Granulocyte colony-stimulating factor also decreased the enzyme secretion but at higher concentrations than those required for granulocyte colony-stimulating factor. In contrast, macrophage colony-stimulating factor increased the enzyme secretion. These colony-stimulating factor-induced effects were specifically neutralized by the corresponding antibodies.
  
  
  CONCLUSIONS
  Colony-stimulating factors may modulate the local concentration of platelet-activating factor in the decidua via their inhibitory or stimulatory effect on the secretion of platelet-activating factor acetylhydrolase, contributing to the regulation of term and preterm parturition at the maternal-fetal interface.
authors.affiliation: Department of Obstetrics and Gynecology, Oita Medical University, Oita, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narahara
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Mine
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawano
authors.forename: John M
authors.initials: JM
authors.lastname: Johnston
authors.forename: Isao
authors.initials: I
authors.lastname: Miyakawa
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002469:Cell Separation; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D017364:Collagenases / Q000378:metabolism; D003115:Colony-Stimulating Factors / Q000494:pharmacology* / Q000502:physiology*; D003656:Decidua / Q000166:cytology* / Q000201:enzymology; D003851:Deoxyribonucleases / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor / Q000276:immunology / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000494:pharmacology; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000494:pharmacology; D008264:Macrophages / Q000201:enzymology*; D010741:Phospholipases A / Q000378:metabolism*; D011247:Pregnancy; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D003115:Colony-Stimulating Factors; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D003851:Deoxyribonucleases; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D006821:Hyaluronoglucosaminidase; D017364:Collagenases