title: [Establishment of method for determining serum beta-hydroxybutyric acid and its meaning in diabetes ketosis acidism].
abstract: OBJECTIVE
  This study was to establish enzyme-rate method for determining serum beta-hydroxybutyric acid, and to discuss its meaning in diabetes ketosis acidism.
  
  
  METHODS
  Enzyme-rate method was used to determine serum beta-hydroxybutyric acid in 60 cases of normals, 30 cases of diabetes, and 8 cases of ketosis acidism by Hitachi 7170A autochemistry analyzer.
  
  
  RESULTS
  The recovery rate is 108%, the linerity extent 0-4.0 mmol.L-1, the coefficient of variation within-run is 2.2% and day-to-day is 2.6%.
  
  
  CONCLUSIONS
  The enzyme-rate method for determining serum beta-hydroxybutyric acid save time, gives accurate results and has wide linerity, so it can used for diagnosis of diabetes ketosis acidism.
authors.affiliation: Department of Clinical Laboratory, Xiangya Hospital, Centeal South University, Changsha 410008, China.
authors.forename: B
authors.initials: B
authors.lastname: Yi
authors.forename: G F
authors.initials: GF
authors.lastname: Wei
authors.forename: Y
authors.initials: Y
authors.lastname: Tang
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood*; D000328:Adult; D016883:Diabetic Ketoacidosis / Q000097:blood* / Q000150:complications; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D020155:3-Hydroxybutyric Acid