Correlates of thrombin generation in patients with advanced prostate cancer.
Thrombin generation is increased in men with advanced prostate cancer. Thrombin has the ability to interact with, and affect the biology of, a variety of cell types including prostate cancer cell lines. We therefore looked for correlations between thrombin generation and other markers of disease activity in spot urine samples obtained from men with advanced prostate cancer. Excretion of part of the prothrombin activation peptide F(1+2) (called here iF2), interleukin-6 (IL-6), the bone turnover marker deoxypyridinoline (DpD), and vascular endothelial growth factor (VEGF) were quantitated in spot urine samples collected from 37 men with hormone-refractory prostate cancer. Following log transformation of the data, significant correlations were found by univariate analysis between the excretion of a marker of thrombin generation (iF2) and IL-6, DpD and VEGF, as well as between IL-6 and DpD or VEGF excretion. No correlation was found between any marker and serum PSA level. After multivariate analysis, a significant correlation remained between thrombin generation and IL-6 excretion. Analysis of a second urine specimen obtained from 19 of the subjects 1 to 7 months after the first also revealed a significant correlation between thrombin generation and IL-6, DpD, and VEGF excretion. These data provide evidence of a correlation between thrombin generation/coagulation system activation and IL-6 generation in patients with cancer. They provide a rationale for studying the effects of inhibitors of thrombin generation upon the biology of prostate cancer.
Lind
Stuart E
SE
Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA. stuart-lind@ouhsc.edu
Caprini
Joseph A
JA
Goldshteyn
Sofia
S
Dohnal
James C
JC
Vesely
Sara K
SK
Shevrin
Daniel H
DH
D000596:Amino Acids / Q000652:urine; D015415:Biomarkers / Q000652:urine; D001777:Blood Coagulation; D006801:Humans; D015850:Interleukin-6 / Q000652:urine; D008297:Male; D010446:Peptide Fragments / Q000652:urine; D011471:Prostatic Neoplasms / Q000097:blood*; D011516:Prothrombin / Q000652:urine; D013917:Thrombin / Q000096:biosynthesis*; D042461:Vascular Endothelial Growth Factor A / Q000652:urine
D000596:Amino Acids; D015415:Biomarkers; D015850:Interleukin-6; D010446:Peptide Fragments; D042461:Vascular Endothelial Growth Factor A; C045879:prothrombin fragment 1.2; D011516:Prothrombin; C036020:deoxypyridinoline; D013917:Thrombin