[Changes of cultured human vein endothelial cells(HVEC) in the presence of therapeutic agents of cavernous hemangioma].
OBJECTIVE
To observe the damages of cultured human vein endothelial cells treated by various drugs.


METHODS
With the identification of EC, damages of which treated by drugs with different concentration were measured with MTT assay respectively, dramatic changes in the morphology of test cells observed under light and electron microscope.


RESULTS
1. According to the different inhibition on proliferation of HVEC, sodium morrhuate exhibited most cytostatic effect for HVEC, and combination of pinyangmycin and hormone produced more significant arrests of growth of HVEC than pinyangmycin used singly. 2. Positive correlation were found between cell contraction and concentration of varied drugs with the absence of structure of junctional integrity. 3. Corresponding pathological changes occurred on HVEC with MTT assay. 4. As the damages of HVEC occurred, therapeutic agents may directly injured substratum of cavernous hemangiomas through enlarged gaps between cells subsequently.


CONCLUSIONS
Evaluation and screening of sclerosing agents for cavernous hemangioma may be feasibly demonstrated with affects on cultured HVEC by different agents.
Guo
J
J
Department of Stomatology, Second Affiliated Hospital, Hunan Medical University.
Wu
H
H
D001761:Bleomycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000473:pathology*; D006392:Hemangioma, Cavernous / Q000473:pathology*; D006801:Humans; D012976:Sodium Morrhuate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014750:Vincristine / Q000494:pharmacology*
D001761:Bleomycin; D014750:Vincristine; D003907:Dexamethasone; D012976:Sodium Morrhuate