Novel fluorophores for single-molecule imaging.
Nonlinear optical chromophores based on dicyanodihydrofuran acceptors paired with amine donors have been found to exhibit sufficiently large fluorescence quantum yields and stability to enable single-molecule detection in polymeric hosts. To illustrate the breadth of this class, six fluorophores are presented, spanning the emission range from 505 to 646 nm. In contrast to conventional single-molecule fluorophores, the new molecules feature sensitivity to local rigidity, large ground-state dipole moments, and large polarizability anisotropies, properties that can be used to design new reporter experiments at the single-molecule level.
Willets
Katherine A
KA
Department of Chemistry, Stanford University, Stanford, California 94305, USA.
Ostroverkhova
Oksana
O
He
Meng
M
Twieg
Robert J
RJ
Moerner
W E
WE
D000588:Amines / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005470:Fluorometry; D005663:Furans / Q000737:chemistry*; D010777:Photochemistry; D013056:Spectrophotometry, Ultraviolet
D000588:Amines; D005456:Fluorescent Dyes; D005663:Furans