Platelet CD62p expression and microparticle in murine acquired immune deficiency syndrome and chronic ethanol consumption.
OBJECTIVE
Abnormal platelet counts have been noticed in acquired immune deficiency syndrome (AIDS) patients. However, the actual state of platelets in AIDS is unclear. We hypothesize that platelets are activated and platelet-derived microparticles increase in murine AIDS.


METHODS
To elucidate the ethanol effects on platelets in murine AIDS, we studied four groups: control, murine AIDS, ethanol, and ethanol plus murine AIDS. Platelet CD62p as a platelet activation marker and CD61(+) microparticles as platelet microparticles (PMPs) were measured by flow cytometry.


RESULTS
Platelets were significantly activated in mice with murine AIDS and chronic ethanol consumption. Increased platelet CD62p expression and increased PMPs were most pronounced in advanced stages of murine AIDS. Chronic ethanol consumption persistently enhanced platelet activation and PMP formation.


CONCLUSIONS
Elevated platelet CD62p and PMPs may represent a pro-thrombotic status that have important pathological consequences.
Chen
Yinhong
Y
Divison of Health Promotion Science, College of Public Health, College of Medicine and The Sarver Heart Center, University of Arizona, Tucson, AZ 85724, USA.
Davis-Gorman
Grace
G
Watson
Ronald R
RR
McDonagh
Paul F
PF
D000428:Alcohol Drinking / Q000009:adverse effects* / Q000097:blood; D000437:Alcoholism / Q000097:blood*; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects; D005260:Female; D005434:Flow Cytometry; D002479:Inclusion Bodies / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D016183:Murine Acquired Immunodeficiency Syndrome / Q000097:blood*; D019007:P-Selectin / Q000097:blood*; D015539:Platelet Activation / Q000187:drug effects*; D010976:Platelet Count
D019007:P-Selectin