title: Two-color flow cytometry with a CD19 gate for the evaluation of bone marrow involvement of B-cell lymphoma.
abstract: The usefulness of two-color flow cytometry with a CD19 gate (CD19-FCM) for detecting clonal B-cells in the bone marrow was evaluated. Since kappa and lambda ratios analyzed in the bone marrow from healthy adults were within the range of 0.5-3.0, outside of this range was regarded as the existence of clonal B-cells. Mixing experiments in vitro showed that 10% or more of clonal B-cells contaminating normal bone marrow cells could be detected using CD19-FCM. Ninety-nine bone marrow samples from 60 patients with B-cell lymphoma were simultaneously analyzed using CD19-FCM and histologic examination of both bone marrow aspiration and core needle biopsy samples. The agreement of results obtained by both examinations was 82% (81/99). Discrepant results were found in 18 samples of 10 patients. All except one sample were positive for CD19-FCM analysis but negative for histologic examination and the conversion of CD19-FCM-positivity to CD19-FCM-negativity was associated with chemotherapy. These results suggest that CD19-FCM is useful for the evaluation of bone marrow involvement of B-cell lymphoma.
authors.affiliation: Division of Cell Transplantation and Transfusion, Jichi Medical School, Minamikawachi, Tochigi 329-0498, Japan. chizuru@jichi.ac.jp
authors.forename: Chizuru
authors.initials: C
authors.lastname: Kawano-Yamamoto
authors.forename: Kazuo
authors.initials: K
authors.lastname: Muroi
authors.forename: Thoru
authors.initials: T
authors.lastname: Izumi
authors.forename: Ken
authors.initials: K
authors.lastname: Saito
authors.forename: Keiya
authors.initials: K
authors.lastname: Ozawa
mesh_terms: D018941:Antigens, CD19 / Q000032:analysis*; D001402:B-Lymphocytes / Q000473:pathology*; D001853:Bone Marrow / Q000473:pathology*; D001856:Bone Marrow Examination; D002999:Clone Cells / Q000473:pathology; D005434:Flow Cytometry / Q000379:methods / Q000592:standards*; D006651:Histocytochemistry; D006801:Humans; D016393:Lymphoma, B-Cell / Q000175:diagnosis / Q000473:pathology*; D009361:Neoplasm Invasiveness; D015203:Reproducibility of Results
chemical_list: D018941:Antigens, CD19