The effects of organophosphate insecticide methidathion on lipid peroxidation and anti-oxidant enzymes in rat erythrocytes: role of vitamins E and C.
The effects of organophosphate insecticide methidathion (MD) on lipid peroxidation and anti-oxidant enzymes and the ameliorating effects of a combination of vitamins E and C against MD toxicity were evaluated in rat erythrocytes. Experimental groups were: control group, MD-treated group (MD), and MD + vitamin E + vitamin C-treated group (MD + Vit). MD and MD + Vit groups were treated orally with a single dose of 8 mg/kg MD body weight at 0 hour. Vitamins E and C were injected at doses of 150 mg/kg body weight, i.m. and 200 mg/kg body weight, i.p., respectively, 30 min after the treatment of MD in the MD + Vit group. Blood samples were taken 24 hours after the MD administration. The level of malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were studied in the erythrocytes. MDA level increased significantly in the MD group compared to the control group (P < 0.05) and decreased significantly in the MD + Vit group compared to the MD group (P < 0.05). The activities of SOD, GSH-Px, and CAT decreased in the MD group compared to the control group (P < 0.05). Only GSH-Px activity increased in the MD + Vit group compared with the MD group. These results suggest that treating rats with MD increases LPO and decreases anti-oxidant enzyme activities in erythrocytes. Furthermore, single-dose treatment with a combination of vitamins E and C 30 min after the administration of MD can reduce LPO caused by MD.
Altuntas
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Department of Biochemistry and Clinical Biochemistry, School of Medicine, Suleyman Demirel University, Isparta 32260, Turkey. irfanaltuntas@hotmail.com
Delibas
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N
Sutcu
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R
D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D002374:Catalase / Q000097:blood; D004338:Drug Combinations; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology*; D016166:Free Radical Scavengers / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D007273:Injections, Intramuscular; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity*; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000097:blood; D009946:Organothiophosphorus Compounds / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood; D014801:Vitamin A / Q000008:administration & dosage / Q000494:pharmacology*
D000975:Antioxidants; D004338:Drug Combinations; D016166:Free Radical Scavengers; D007306:Insecticides; D009946:Organothiophosphorus Compounds; D014801:Vitamin A; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid; C005828:methidathion