Experimental investigation on neural cell survival after dielectrophoretic trapping.
Negative dielectrophoretic forces can effectively be used to trap cortical rat neurons. The creation of dielectrophoretic forces requires electric fields of high non-uniformity. High electric field strengths, however, can cause excessive membrane potentials by which cells may unrecoverably be changed or it may lead to cell death. In a previous study it was found that cells trapped at 3 Vtt/14 MHz did not change morphologically as compared to cells that were not exposed to the electric field. This study investigates the viability of fetal cortical rat neurons after being trapped by negative dielectrophoretic forces at frequencies up to 1 MHz. A planar quadrupole micro-electrode structure was used for the creation of a non-uniform electric field. The sinusoidal input signal was varied in amplitude (3 and 5 Vtt) and frequency (10 kHz-1 MHz). The results presented in this paper show that the viability of dielectrophoretically trapped postnatal cortical rat cells was greatly frequency dependent. To preserve viability frequencies above 100 kHz (at 3 Vtt) or 1 MHz (5 Vtt) must be used.
Heida
T
T
Institute of BioMedical Technology, Department of Biomedical Engineering, Faculty of Electrical Engineering, University of Twente, Enschede, The Netherlands. t.heida@el.utwente.nl
Rutten
W L C
WL
Marani
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D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology / Q000502:physiology; D004553:Electric Conductivity; D004586:Electrophoresis / Q000379:methods; D004867:Equipment Design; D005333:Fetus / Q000502:physiology; D008839:Microelectrodes; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D051381:Rats; D012815:Signal Processing, Computer-Assisted