[A study of on the transport of neurotrophin-4 in facial nerve].
OBJECTIVE
This is a study on the transport information of neurotrophin-4 (NT-4) in facial nerve using radioactive tracer technique.


METHODS
After one lateral facial nerve trunk of adult rabbit was transected, a silicone chamber was inserted between the stumps, and 7.4 MBq of 131I-NT-4 was administered into the chamber. At the distinct moment of post-injection, the head of one rabbit was imaged at coronary position, the bilateral facial nerve trunk and brain-stem of the others were collected and counted respectively. After 1 mg of brain-derived neurotrophic factor (BDNF) and 7.4 MBq of 131I-NT-4 were administered into the chamber of one rabbit, the rabbit was imaged at coronary position of head at distinct moment.


RESULTS
5.08% of 131I-NT-4 was transported into facial nerve trunk at 4 h of post-injection. 131I-NT-4 presented high peak in facial nerve trunk during the period of 8, 12 h, the peak values were 20.58% and 22.74% respectively. 34.75% and 45.57% of 131I-NT-4 were transported into the brain-stem of experimental side at 8 h and 12 h respectively. The transport of 131I-NT-4 was markedly restrained by BDNF in facial nerve.


CONCLUSIONS
NT-4 displays a receptor-mediated retrograde transport in facial nerve.
Li
Y
Y
Department of Nuclear Medicine, First Affiliated Hospital, WCUMS, Chengdu 610041, China.
Yang
X
X
Li
L
L
Wang
Q
Q
He
G
G
Gao
B
B
Lin
D
D
D000818:Animals; D001692:Biological Transport; D001933:Brain Stem / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology; D005154:Facial Nerve / Q000378:metabolism* / Q000601:surgery; D009414:Nerve Growth Factors / Q000378:metabolism*; D009416:Nerve Regeneration; D011817:Rabbits
D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; C073922:neurotrophin 4