title: Melanocyte detachment after skin friction in non lesional skin of patients with generalized vitiligo.
abstract: BACKGROUND
  In vitiligo, melanocytes are gradually lost in depigmented macules of the skin. The disappearance of melanocytes has, however, not been clearly observed and consequently the aetiology of the disease (autoimmune, neural, cytotoxic) is still elusive. The starting point of vitiligo macules is frequently determined by local conditions such as wounds and excoriations, but may also follow minor traumas such as pressure or repeated friction. This prominent feature is often neglected.
  
  
  OBJECTIVE
  To clarify the biological consequences of repeated friction on the attachment and survival of melanocytes in non lesional vitiligo skin.
  
  
  METHODS
  Light reproducible skin friction was performed for 4 min on the volar forearm of 18 patients with extensive vitiligo and five controls with normal healthy skin. Biopsies from the test area and control skin were taken at 1, 4, 24 and 48 h following friction. Serial sections were examined with standard light microscopy, transmission electron microscopy, histochemistry and immunohistochemistry (dihydroxyphenylalanine, HMB-45, E-cadherin and an early apoptosis marker, M30 cytoDEATH antibody).
  
  
  RESULTS
  The observation of sections at 1 and 48 h after friction on vitiligo skin and at all time points in controls revealed no changes. In contrast, in vitiligo skin at 4 and 24 h after friction, several melanocytes had undergone detachment and were found in various suprabasal positions, including the stratum spinosum, granular layer, and within and outside the stratum corneum.
  
  
  CONCLUSIONS
  Detachment and transepidermal elimination of melanocytes following minor mechanical trauma in non lesional vitiligo skin is probably the cause of depigmentation occurring in the isomorphic response (Koebner phenomenon). We propose that transepidermal elimination of melanocytes in vitiligo should be regarded as a possible mechanism of chronic loss of pigment cells, perhaps previously damaged by another process.
authors.affiliation: Department of Dermatology, Centre Hospitalier Universitaire de Bordeaux, Hôpital Saint André, 1 rue Jean Burguet, France. ygauthier001@cegetel.rss.fr
authors.forename: Y
authors.initials: Y
authors.lastname: Gauthier
authors.forename: M
authors.initials: M
authors.lastname: Cario-Andre
authors.forename: S
authors.initials: S
authors.lastname: Lepreux
authors.forename: C
authors.initials: C
authors.lastname: Pain
authors.forename: A
authors.initials: A
authors.lastname: Taïeb
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis; D001706:Biopsy; D015820:Cadherins / Q000032:analysis; D002448:Cell Adhesion; D005542:Forearm; D017276:Friction; D006801:Humans; D008544:Melanocytes / Q000648:ultrastructure*; D008875:Middle Aged; D012867:Skin / Q000737:chemistry; D014820:Vitiligo / Q000378:metabolism / Q000473:pathology*
chemical_list: D015820:Cadherins