[Study on in vitro culture and cryopreservation by vitrification of blastocysts derived from single mouse 2-cell embryos' blastomeres].
The present experiments were designed to study the effects of glucose, EDTA, glutamine on the in vitro development of single blastomeres from 2-cell embryos in mouse, and the efficiency of cryopreservation of blastocysts from single blastomers with different vitrification. Single blastomeres derived from female ICR x male BDF1 2-cell embryos were cultured in mKRB with or without glucose, EDTA and glutamine, respectively. The expanded blastocyst rates were significantly different between in mKRB with glucose and without glucose (34% vs 65%); The blastomeres were cultured in mKRB with EDTA and glutamine but glucose, the expanded blastocyst rate (90%) was significantly higher than other groups. The blastocysts derived from single blastomeres were vitrified in liquid nitrogen after equilibration in GFS40 for 0.5-2 min, the survival rate 24%-51%. The blastocysts were pretreated in mPBS with 10% glycerol for 5 min, followed by exposure to GFS40 at 25 degrees C for 0.5 min, then vitrified in liquid nitrogen(two-step method), the survival rate was 61%. However, the survival rates increased to 64% and 70% when the blastocysts were vitrified(one-step method) ater equilibration in EFS40 at 25 degrees C for 0.5-1 min.
Meng
Q G
QG
College of Animal Science, China Agricultural University, Beijing 100094.
Zhu
S E
SE
Zeng
S M
SM
Zhang
Z C
ZC
Li
S R
SR
Wang
D P
DP
Li
J Q
JQ
D000818:Animals; D001755:Blastocyst; D001757:Blastomeres / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media; D046508:Culture Techniques; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR
D003470:Culture Media