[Cloning expression of a novel human protein-disulfide isomerase like cDNA].
Using the large scale sequencing, a novel human cDNA of 1,645 bp was screened from the cDNA library of human fetal brain. The cDNA contains an ORF encoding a 296-aa protein with a calculated molecular weight of 34.0 KD. Compared with that of current sequence databases, the putative protein was found to have 36% homology with protein-disulfide isomerase (PDI). So this cDNA was named PDI like (PDI-L) gene. Multiple tissue Northern blot analysis shows that PDI-L cDNA is expressed in heart, brain, liver, kidneys and so on. The ORF fragment of PDI-L cDNA was inserted into reconstructed pBV220 expression vehicle and its predicted expressive protein was obtained.
Huang
Jin
J
College of Medicine, Shihezi University, Xinjiang 832003.
Yang
Lei
L
Zhang
Yulan
Y
Chen
Xuanmao
X
Ying
Kang
K
Xie
Yi
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D019704:Protein Disulfide-Isomerases / Q000096:biosynthesis / Q000235:genetics*
D018076:DNA, Complementary; D019704:Protein Disulfide-Isomerases