title: Using an aplysia two-hybrid system to examine the interactions between transcription factors involved in long-term facilitation in the nervous system of aplysia.
abstract: Interactions between ApCREB1a, ApCREB2, and ApC/EBP have been studied using conventional methods such as the yeast two-hybrid system. However, it is unclear whether these memory-related transcription factors actually interact in the native environment in neurons. To clarify this question, we have developed an Aplysia two-hybrid system and found, consistent with previous studies that ApCREB2 interacts with ApCREB1a and ApC/EBP, and that ApC/EBP forms homodimers. We have also found that ApCREB1a and ApC/EBP do not interact. Therefore, our study shows that formerly described interactions between the proteins actually occur in the Aplysia neurons and that interactions between these transcription factors are specific.
authors.affiliation: National Research Laboratory, Institute of Molecular Biology and Genetics, School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 151-742, Korea.
authors.forename: Jung-Hwan
authors.initials: JH
authors.lastname: Choi
authors.forename: Jin-A
authors.initials: JA
authors.lastname: Lee
authors.forename: Seok-Won
authors.initials: SW
authors.lastname: Yim
authors.forename: Chae-Seok
authors.initials: CS
authors.lastname: Lim
authors.forename: Chi-Hoon
authors.initials: CH
authors.lastname: Lee
authors.forename: Young-Don
authors.initials: YD
authors.lastname: Lee
authors.forename: Dusan
authors.initials: D
authors.lastname: Bartsch
authors.forename: Eric R
authors.initials: ER
authors.lastname: Kandel
authors.forename: Bong-Kiun
authors.initials: BK
authors.lastname: Kaang
mesh_terms: D000818:Animals; D001048:Aplysia; D017362:Cyclic AMP Response Element-Binding Protein; D008156:Luciferases / Q000378:metabolism; D008845:Microinjections; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009420:Nervous System / Q000201:enzymology / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: C097204:ApCREB2 protein, Aplysia californica; D017362:Cyclic AMP Response Element-Binding Protein; D009419:Nerve Tissue Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D008156:Luciferases; D001616:beta-Galactosidase