title: [p16 and cyclinD1 protein expression and p16 gene mutation in primary human hepatocellular carcinoma].
abstract: OBJECTIVE
  To elucidate the relationship between expression of p16 and cyclinD1 proteins and evaluate the role of p16 gene exon 2 mutation in hepatocellular carcinogenesis.
  
  
  METHODS
  Streptavidin-peroxidase conjugated method(SP) was used to detect expressions of p16 and cyclinD1 proteins in 44 cases of hepatocellular carcinoma(HCC), and polymerase chain reaction single-strand conformation polymorphism analysis(PCR-SSCP) to detect p16 gene mutation in exon 2 in 12 cases of HCC and liver tissues adjacent to HCC.
  
  
  RESULTS
  Expression rate and positive signal intensity of p16 protein in HCC were significantly lower(P < 0.01) and those of cyclinD1 protein in HCC were significantly higher (P < 0.05) than those in pericarcinomatous tissues. Of 12 fresh HCC tissues, p16 gene mutation in exon 2 was found in 2 cases, whereas that was not found in pericarcinomatous tissues.
  
  
  CONCLUSIONS
  1. Inactivation or/ and deletion of p16 protein may be one of important reasons which result in proliferation unbalance of cells. 2. p16 gene mutation in exon 2 presents in HCC, but it does not frequently occur in Chinese hepatocarcinogenesis. 3. p16 gene abnormality and cyclinD1 over expression may coact in HCC.
authors.affiliation: Department of Pathology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: G X
authors.initials: GX
authors.lastname: Huang
authors.forename: R X
authors.initials: RX
authors.lastname: Cheng
authors.forename: D Y
authors.initials: DY
authors.lastname: Feng
mesh_terms: D000293:Adolescent; D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism; D019938:Cyclin D1 / Q000096:biosynthesis* / Q000235:genetics; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis / Q000235:genetics; D005091:Exons; D005260:Female; D019942:Genes, p16; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009154:Mutation
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D019938:Cyclin D1