[Expression of rice dwarf virus outer coat protein gene(S8) in insect cells].
Outer coat protein gene(S8) of RDV was cloned into the transfer vector pVL 1393 to construct a recombinant vector pVL1393-S8. The recombinant vector pVL1393-S8 and the linear baculovirus RP23. LacZ were cotransfected into sf9 cells to produce the recombinant virus RP23-S8. RP23-S8 infected sf9 cells were collected and analysed by SDS-PAGE and Western-blot. The results showed that the S8 gene of RDV was expressed in sf9 cells and the expression level of sf9 cells was higher between 72-96 h after infected.
Li
S
S
Department of Biotechnology, College of Life Science, Beijing Univ., Beijing, 100871, China.
Liu
H
H
Chen
Z
Z
Li
Y
Y
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D036022:Capsid Proteins / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D015870:Gene Expression; D005814:Genes, Viral; D005822:Genetic Vectors; D012275:Oryza / Q000821:virology; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D012087:Reoviridae / Q000235:genetics*; D018411:Spodoptera / Q000166:cytology / Q000378:metabolism*; D014169:Transformation, Bacterial; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
D036022:Capsid Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins; C071650:S8 protein, rice gall dwarf virus