Lymphocyte subpopulations in hypertrophied adenoid in children with otitis media with effusion.
Otitis media with effusion (OME) usually coexist with the adenoid hypertrophy. The aim of this study was a quantitative evaluation of the lymphocyte subpopulations in hypertrophied adenoid in children with persistent (ove three months) otitis media with effusion. We used flow cytometry for study the subpopulations of the lymphocytes in the pharyngeal adenoid during otitis media in the children, alternative group consist of children with simple adenoid hypertrophy. The monoclonal antibodies selected were specific markers for T lymphocytes (CD3), T-helper cells (CD4), T-suppressor cells (CD8), B cells (CD19) and NK cells (CD56). There were also analyzed T activation markers: HLA-DR and CD25. The percentage of NK cells was significantly decreased in the group of younger children with otitis media with effusion (0.81 +/- 0.51) in comparison to older ones (1.40 +/- 0.59%). Also, in the group of younger children with adenoid hypertrophy, the NK cells percentage was significantly lower (0.85 +/- 0.37%) than in the group of older children--(1.46 +/- 0.83). In both subgroups of younger children the percentage of lymphocytes T with HLA-DR activation marker was close, but it was significantly lower in the group of older children with otitis media with effusion (4.28 +/- 3.40%) in comparison to the group of older children with adenoid hypertrophy (5.03 +/- 3.00%).
Wysocka
Jolanta
J
Department of Pediatric Laboratory Diagnostics, Medical Academy of Bialystok, Białystok, Poland. zldpam@poczta.fm
Hassmann
Elzbieta
E
Kasprzycka
Edwina
E
Musiatowicz
Marcin
M
Lipska
Alina
A
D000233:Adenoidectomy; D000234:Adenoids / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D000293:Adolescent; D018941:Antigens, CD19 / Q000032:analysis; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D015415:Biomarkers / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D046508:Culture Techniques; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006984:Hypertrophy / Q000175:diagnosis; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D010034:Otitis Media with Effusion / Q000276:immunology* / Q000601:surgery; D011336:Probability; D012008:Recurrence; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D018941:Antigens, CD19; D015415:Biomarkers; D017252:CD3 Complex