[Separation of standard proteins and actual samples using cross-axis counter current chromatography].
A system of m (12.5% PEG8000): m (25% K2HPO4) = 1:1 was used to separate two kinds of standard proteins, glycoprotein in Morchella esculenta (L.) and glycoprotein in Lycium barbarum (L.), the upper phase was used as stationary phase, and the lower phase was used as mobile phase. The revolution speed was 500 r/min and the flow rate was 60 mL/h. In comparing with high performance liquid chromatography, cross-axis counter current chromatography can increase load capacity without loss of resolution. The advantage of this method was verified, indicating that cross-axis counter current chromatography was a useful method for biopolymer separation.
Wei
Y
Y
Beijing Research Center for Separation & Purification Technologies of Natural Products, Beijing Institute of New Technology and Application, Beijing 100035, China. yun-wei@263.net
Zhang
T Y
TY
Zhang
S
S
Liu
Q H
QH
D001203:Ascomycota / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods; D005285:Fermentation; D006023:Glycoproteins / Q000302:isolation & purification*; D032305:Lycium / Q000737:chemistry*; D011108:Polymers / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification
D006023:Glycoproteins; D011108:Polymers; D011506:Proteins