Isolation and structure of the cytotoxic cycloheptapeptide phakellistatin 13.
A new cyclic heptapeptide phakellistatin 13 (1) had been isolated from the sponge Phakellia fusca Thiele, collected at Yongxing Island of China. Its structure was elucidated as cyclo-(Pro1-Trp-Leu-Thr-Pro2-Gly-Phe) on the basis of MS, UV, IR, and high-field NMR (600 MHz) analysis. The compound was significantly cytotoxic against the human hepatoma BEL-7404 cell line with an ED(50) < 10(-2) microg/mL.
Li
Wen-Lin
WL
Research Center for Marine Drugs, School of Pharmacy, Second Military Medical University, 325 Gou-He Road, Shanghai 200433, People's Republic of China.
Yi
Yang-Hua
YH
Wu
Hou-Ming
HM
Xu
Qiang-Zhi
QZ
Tang
Hai-Feng
HF
Zhou
Da-Zheng
DZ
Lin
Hou-Wen
HW
Wang
Zhong-Hua
ZH
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular; D002681:China; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011161:Porifera / Q000737:chemistry*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D010456:Peptides, Cyclic; C474532:phakellistatin 13