[Effects of two growth factors combination on the proliferation and differentiation of human osteoblast-like cells].
OBJECTIVE
To examine the effects of platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF-II) and type beta transforming growth factor(TGF-beta) which were believed to have biological effects on bone cells on human osteoblast-like cells.


METHODS
3H-TdR, 3H-Proline and alkaline phosphatase of the osteoblast-like cells were tested to study the combinating effects of different growth factors on the proliferation and differentiation of osteoblast-like cells.


RESULTS
The combination of different growth factors had promotive effect and synergic interact on proliferation and differentiation of osteoblast-like cells.


CONCLUSIONS
The phenomena indicate that the combination of two growth factors will enhance the biological effects of bone formation.
Tian
W
W
College of Stomatology, West China University Medical Sciences.
Wang
D
D
Qiao
J
J
Chen
W
W
D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004357:Drug Synergism; D004622:Embryo, Mammalian; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000008:administration & dosage* / Q000494:pharmacology; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000008:administration & dosage* / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000008:administration & dosage* / Q000494:pharmacology
D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D007335:Insulin-Like Growth Factor II