[Determination of aspirin and free salicylic acid in lysinipirine injection by high performance liquid chromatography].
The contents of aspirin and free salicylic acid in lysinipirine injection were determined by high performance liquid chromatography (HPLC). A Hypersil BDS C18 column was used with the mobile phase of methanol-water-acetic acid (35:65:3, volume ratio) and the detection wavelength of 280 nm. The average recoveries of aspirin and salicylic acid added were 99.27% (RSD = 0.8%) and 99.61%(RSD = 1.3%), respectively. The calibration curves had good linearity in the range of 0.028 g/L -0.141 mg/L and 0.77 mg/L -3.85 mg/L, and the correlation coefficients were 0.9999 and 0.9998 for aspirin and salicylic acid respectively.
Dong
Yu
Y
Dalian Institute for Drug Control, Dalian 116021, China.
Zhao
Yuan-zheng
YZ
Zhang
Yi-na
YN
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry; D001241:Aspirin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry*; D020156:Salicylic Acid / Q000032:analysis*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D008239:Lysine; D020156:Salicylic Acid; D001241:Aspirin; C010395:acetylsalicylic acid lysinate