VEGF induced hyperpermeability in bovine aortic endothelial cell and inhibitory effect of salvianolic acid B.
OBJECTIVE
To examine the effect of recombinant human vascular endothelial growth factor (VEGF) on the low density lipoprotein (LDL) permeability of bovine aortic endothelial cells (BAEC) and the inhibitory effect of salvianolic acid B in vitro.


METHODS
The confluence BAEC monolayers were cultured with normal medium and with medium containing VEGF or salvianolic acid B at various concentrations and for various time periods. The iodine labeled LDL flux across the monolayers was then performed, and radioactivity was measured by SN-695 automatic liquid scintillation counter.


RESULTS
Addition of purified human recombinant VEGF to the BAEC monolayers could significantly increase the permeability of the monolayer to 125I-LDL (P < 0.01). The permeability-increasing activity of VEGF on the BAEC monolayers was both dose and time dependent. Salvianolic acid B could markedly inhibit the VEGF-induced hyperpermeability in BAECs (P < 0.01).


CONCLUSIONS
VEGF plays a role in the formation and development of atherosclerosis, and salvianolic acid B has inhibitory effect on VEGF-induced hyperpermeability in BAEC.
Qui
Y
Y
Department of Pharmacology, Second Medical Military University, Shanghai, China.
Rui
Y C
YC
Zhang
L
L
Li
T J
TJ
Zhang
W D
WD
D000818:Animals; D001011:Aorta / Q000166:cytology; D001572:Benzofurans / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects*; D002417:Cattle; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D016228:Endothelial Growth Factors / Q000037:antagonists & inhibitors*; D004730:Endothelium, Vascular / Q000166:cytology*; D036341:Intercellular Signaling Peptides and Proteins; D008077:Lipoproteins, LDL / Q000378:metabolism; D008222:Lymphokines / Q000037:antagonists & inhibitors*; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D053630:Depsides
D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008077:Lipoproteins, LDL; D008222:Lymphokines; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; C076944:salvianolic acid B; D053630:Depsides