Eosinophilic major basic protein and interleukin-5 in eosinophilic myositis.
To determine whether eosinophils play a critical role in muscle fiber damage in patients with eosinophilic myositis (EM). We investigated expression of eosinophilic major basic protein (MBP) and interleukin (IL)-5 at the protein and mRNA levels in muscle biopsies from three patients with idiopathic EM. MBP deposits were found on the surface of eosinophils and muscle fibers surrounded by the eosinophils. Reverse transcriptase-polymerase chain reaction analysis showed increased IL-5 expression in EM muscle but not in control muscle. These results suggest that IL-5 induces local accumulation of eosinophils and their release of MBP. The secreted proteins adhere to the muscle fiber membrane, resulting in muscle damage.
Murata
K
K
Department of Neurology, Nara Medical University, Nara, Japan. kemurata@naramed-u.ac.jp
Sugie
K
K
Takamure
M
M
Fujimoto
T
T
Ueno
S
S
D000328:Adult; D001798:Blood Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D047091:Eosinophil Granule Proteins; D004802:Eosinophilia / Q000378:metabolism* / Q000473:pathology; D004804:Eosinophils / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D015848:Interleukin-5 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D009220:Myositis / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis; D012260:Ribonucleases
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D015848:Interleukin-5; D012333:RNA, Messenger; D012260:Ribonucleases