[Characterization and phylogenetic analysis of the new species of the Leucobacter tropicalis sp. nov].
A new aerobic, gram-positive, non-sporulating, rod-shaped organism is described. Strain F-8 was isolated from tropical soil, and has the following characteritics: the menaquinone contains a side chain with 11 isoprenyl units(MK-11); 2,4-diaminobutyric acid, glutamic acid, alanine, glycine and a small amount of gamma-aminobutyric acid present in the cell wall at a molar ratio of 0.77:1.0:1.66:0.95. The major cellular sugar were rhamnose, galactose and glucose. The G + C content of DNA is 68 mol%. The 16S rRNA gene was amplified, cloned and sequenced, a phylogenetic tree was constructed on the 16S rRNA gene sequences. The tree clearly indicated that strain F-8 forming the same lineage with leucobacter komagatae, the level of binary sequence similarity between strain F-8 and L. komagatae is 96%. Based on the characteristics, allow the placement of strain F-8 into the genus Leucobacter, but F-8 showed some differences in biochemical and physical characters from the reported species L. komagae. A new species name, Leucobacter tropicalis sp. nov. was proposed for this isolate. The type strain is designated F-8.
Liu
H
H
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
Zhou
P
P
Yokota
A
A
Sugiyanma
J
J
D001483:Base Sequence; D006093:Gram-Positive Asporogenous Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S