Effects of short-acting hypnotics on sleep latency in rats placed on grid suspended over water.
The present study was performed to develop a new sleep disturbance model for evaluating hypnotic potencies by placing rats on a grid suspended over water up to 1 cm under the grid surface. When rats were placed on the grid, significant increases in sleep latency and amount of wakefulness were observed compared with those of rats placed on sawdust. However, the amounts of non-rapid eye movement (non-REM) sleep and rapid eye movement (REM) sleep of rats placed on the grid were significantly decreased compared with those of rats placed on sawdust. Four short-acting hypnotics (triazolam, zopiclone, brotizolam, lormetazepam) caused significant decreases in sleep latency, and the effects of hypnotics in rats placed on the grid were more potent than those in rats placed on sawdust. In conclusion, the present model can serve as a new sleep disturbance model and may also be useful for evaluating the sleep-inducing effects of short-acting hypnotics.
Shinomiya
Kazuaki
K
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-naka 1-1-1, Okayama 700-8530, Japan.
Shigemoto
Yuki
Y
Okuma
Chihiro
C
Mio
Mitsunobu
M
Kamei
Chiaki
C
D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology; D053961:Azabicyclo Compounds; D001381:Azepines / Q000494:pharmacology; D001569:Benzodiazepines; D004305:Dose-Response Relationship, Drug; D004569:Electroencephalography; D004576:Electromyography; D006993:Hypnotics and Sedatives / Q000494:pharmacology*; D008140:Lorazepam / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D010879:Piperazines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012890:Sleep / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors; D014229:Triazolam / Q000494:pharmacology; D014867:Water / Q000502:physiology*
D014151:Anti-Anxiety Agents; D053961:Azabicyclo Compounds; D001381:Azepines; D006993:Hypnotics and Sedatives; D010879:Piperazines; C515050:zopiclone; D014867:Water; D001569:Benzodiazepines; D014229:Triazolam; C020960:brotizolam; C023842:lormetazepam; D008140:Lorazepam