[Molecular cloning of rat OB gene and its expression in Escherichia coli].
In order to provide rat OB gene product for studying the relationship between obesity and noninfectious diseases, rat OB cDNA was amplified by RT-PCR technique. 460 bp fragment of OB cDNA was subcloned into EcoRI/BamHI site of plasmid pUC 19. Sequence analysis of OB cDNA revealed that the translation reading frame was identical with that reported in the literature. Thereby plasmid pBV220-rOB was constructed and the specific expression of OB gene in E. coli identified by SDS-PAGE electrophoresis was obtained.
Zhang
T
T
Beijing Institute of Geriatrics, Ministry of Health, Beijing 100730.
Lin
Y
Y
Han
C
C
Jin
J
J
Yao
W
W
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D020738:Leptin / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D020738:Leptin; D011994:Recombinant Proteins