[Construction of mucosal vaccine derived from HBV surface antigen epitope A and the assay of its immunogenicity].
The fusion gene of HBV(adr) surface antigen epitope and B subunit of Cholera toxin was constructed and expressed successfully in E. coli at high yield. After denaturation-renaturation process, SDS-PAGE analysis showed that most of the renatured product reassociated in a pentameric form which was the same as natural CTB. Western blot analysis indicated that the immunogenicity of HBsAg antigen epitope was conserved. Moreover, ELISA analysis of the sera of orally, intranasally and intraperitoneally immunized mice showed that the circulating IgG antibodies to HBsAg were developed. The results may be helpful for constructing the novel mucosal vaccine with high efficacy.
Liu
X
X
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Yang
G
G
Qiu
B
B
Tian
P
P
D000818:Animals; D002772:Cholera Toxin / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D004926:Escherichia coli / Q000378:metabolism; D005814:Genes, Viral; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D017325:Hepatitis B Vaccines; D018928:Immunity, Mucosal / Q000276:immunology*; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D016056:Immunodominant Epitopes; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D002772:Cholera Toxin