Evaluation of Kluyveromyces marxianus as a source of yeast autolysates.
Cells of Kluyveromyces marxianus FII 510700 and Saccharomyces cerevisiae CBS 1907 were autolysed in phosphate buffer, pH 4.5, for a maximum of 10 days to compare chemical changes that occur in the carbohydrate, protein, amino acid and nucleic acid content. Approximately 2.2-3% carbohydrate, 9.5-12% protein, 0.6-1.0% DNA and 6-7% RNA were recovered in the autolysates. The main amino acids were beta-alanine, phenylalanine, cysteine, methionine, glutamic acid and isoleucine. No significant differences in the yeast autolysates of K. marxianus and S. cerevisiae were observed. Consequently, K. marxianus produced from lactose-based media has potential as a source of yeast autolysates used in the food industry.
Lukondeh
Tredwell
T
School of Civil and Environmental Engineering, The University of New South Wales, UNSW-Sydney 2052, Australia. t.lukondeh@unsw.edu.au
Ashbolt
Nicholas J
NJ
Rogers
Peter L
PL
D000596:Amino Acids / Q000378:metabolism; D050260:Carbohydrate Metabolism; D004271:DNA, Fungal / Q000378:metabolism; D019649:Food Industry / Q000379:methods; D005656:Fungal Proteins / Q000378:metabolism; D007716:Kluyveromyces / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D012331:RNA, Fungal / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*
D000596:Amino Acids; D004271:DNA, Fungal; D005656:Fungal Proteins; D012331:RNA, Fungal