[Plate theory model under non-ideal chromatography].
The mobile phase in chromatography is regarded as to be composed of many continual plates, whose height is the same as that of one plate in the stationary phase. The solute in initial concentration of mobile phase is seen to be concentrated in the first plate. The solute is exchanged dynamically between the mobile phase and stationary phase. When the mobile phase passes the distance of one plate in the stationary phase, the solute cannot be immediately released from the stationary phase to mobile phase, because of the dynamic factors of chromatographic system. The solute distribution between mobile and stationary phases is controlled by two factors, i.e. the equilibrium coefficient P, which represents chromatographic thermodynamic properties, and release probability factor alpha, which represents chromatographic dynamic properties. This process is considered as non-ideal chromatography. In the view of statistics, authors studied the influences integrated of the release property factor and equilibrium coefficient factor upon chromatographic distribution, and simulated the chromatographic distribution curve by means of computer. As a result, a new distribution model has been established, the distribution equation under the non-ideal chromatography was deduced and, the integrative action of the release probability and the equilibrium coefficient was revealed. The release probability factor can be regarded as an index in chromatography and the peak width can be deduced and hence the resolution can be calculated through this index.
Wang
Shao-kun
SK
Jiangsu Provincial Institute for Drug Control, Nanjing 210008, China. wangshaokun@yahoo.com
Xia
Yun
Y
Wang
Sheng-li
SL
D002845:Chromatography / Q000295:instrumentation / Q000379:methods; D008962:Models, Theoretical; D011336:Probability; D012997:Solvents; D013816:Thermodynamics
D012997:Solvents