Monitoring the colonization of monuments by bacteria: cultivation versus molecular methods.
Building materials commonly used in wall paintings and monuments (mortar, limestone and sandstone) were inoculated with an artificial consortium composed of 14 microorganisms and incubated for 6 months at 28 degrees C. The colonization of the different materials by the consortium was investigated. Culture-independent techniques revealed the presence of a diversity of bacteria, whereas culture-dependent techniques yielded mainly spore-forming bacteria. The data suggest that plating leads to an overestimation of the number of spore-forming bacteria with respect to quiescent vegetative forms; the latter are less easily cultured, but are readily detected by culture-independent techniques.
Laiz
Leonila
L
Instituto de Recursos Naturales y Agrobiologia, CSIC, Apartado 1052, Avenida Reina Mercedes, 10, 41012 Sevilla, Spain.
Piñar
Guadalupe
G
Lubitz
Werner
W
Saiz-Jimenez
Cesareo
C
D001108:Architecture; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development*; D015373:Bacterial Typing Techniques; D003252:Construction Materials; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004783:Environmental Microbiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D003470:Culture Media; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S