[Producing characterization of constitutive-form laccase by Basidiomycete].
A constitutive-laccase-producing fungus was gained from 20 strains of different sources with plate method. Laccase activity reached 0.58 IU/mL when the strain grew on DC medium with shaking cultivation, which was superior to stationary method by 4 times. The enzyme production paralleled with biomass in early 8 days, subsequently activities declined slightly though its biomass continued upward. Tyrosine and tryptone at the concentration of 4 g/L improved the enzyme production, reaching 2.49 and 2.0 IU/mL respectively. Some tested chemical agents seemed not to contribute to the increase of the enzyme production effectively except for syringaldazine and o-tolidine. Heatshock at 40 degrees C for 1 h made 1.3 times more enzyme expression.
Chao
Y
Y
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
Ye
J
J
Qian
S
S
D001487:Basidiomycota / Q000187:drug effects / Q000201:enzymology*; D001560:Benzidines / Q000494:pharmacology; D018533:Biomass; D003470:Culture Media; D006835:Hydrazones / Q000494:pharmacology; D042845:Laccase / Q000096:biosynthesis* / Q000378:metabolism; D010461:Peptones / Q000494:pharmacology; D013696:Temperature; D014443:Tyrosine / Q000494:pharmacology
D001560:Benzidines; D003470:Culture Media; D006835:Hydrazones; D010461:Peptones; C009370:syringaldazine; D014443:Tyrosine; C009501:2-tolidine; C001830:tryptones; D042845:Laccase