Development of the single-cell MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) mass-spectroscopic assay.
MALDI-TOF mass spectrometry was used to detect intracellular molecules from a single intact cell on monolayers of other cells. Intracellular molecules, e.g., histamine, were gradually increased in a mouse bone marrow-derived mast cell by a maturation process. A single cell was captured by a microsuction pipette, and the mass spectra of intracellular histamine were measured directly. Finally, the time course of the intracellular molecular contents and the maturation stage from a single cell were estimated.
Shimizu
Masakazu
M
Department of Analytical Life Science, Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
Ojima
Noriyuki
N
Ohnishi
Hitoshi
H
Shingaki
Tomoteru
T
Hirakawa
Yasuyuki
Y
Masujima
Tsutomu
T
D016475:3T3 Cells; D000818:Animals; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D005347:Fibroblasts / Q000166:cytology; D008407:Mast Cells / Q000166:cytology; D051379:Mice; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D017077:Culture Media, Conditioned