title: Calcium signalling in and around the nuclear envelope.
abstract: We have compared calcium mobilization by Ins(1,4,5) P(3)(IP(3)), cADP-ribose (cADPR) and nicotinic acid-adenosine dinucleotide phosphate (NAADP) from the envelope of isolated nuclei with the calcium signalling in intact isolated pancreatic acinar cells. Ca(2+) uptake and release were studied with calcium-sensitive fluorescent probes. In the present study, we have shown that all calcium messengers induce Ca(2+) release from the nuclear envelope. Pre-treatment of nuclei with thapsigargin completely abolished the responses to the calcium messengers, indicating that Ca(2+) stores in isolated nuclei are thapsigargin-sensitive. Using different pharmacological tools, we show that Ca(2+) release from pancreatic nuclei is unlikely to occur from stores other than those with endoplasmic reticulum characteristics. We conclude that all three calcium messengers can release Ca(2+) from pancreatic acinar nuclear stores, as previously shown for IP(3) and cADPR. It would appear that NAADP releases Ca(2+) from the same IP(3)- and cADPR-sensitive stores with endoplasmic reticulum characteristics.
authors.affiliation: Medical Research Council Secretory Control Research Group, Physiological Laboratory, University of Liverpool, Liverpool L69 3BX, UK. j.v.gerasimenko@liv.ac.uk
authors.forename: J
authors.initials: J
authors.lastname: Gerasimenko
authors.forename: Y
authors.initials: Y
authors.lastname: Maruyama
authors.forename: A
authors.initials: A
authors.lastname: Tepikin
authors.forename: O H
authors.initials: OH
authors.lastname: Petersen
authors.forename: O
authors.initials: O
authors.lastname: Gerasimenko
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D051379:Mice; D009685:Nuclear Envelope / Q000378:metabolism*; D010179:Pancreas / Q000166:cytology; D015398:Signal Transduction; D019284:Thapsigargin / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D019284:Thapsigargin; D002118:Calcium