[Screening DPPH radical scavengers from Monascus sp].
A strain belongs to Monascus sp. was separated from hongqu(Chinese beni-coji) which was used to brew red wine in Changxin county, Zhejiang province, and its DPPH radical scavenging metabolites were studied. Results showed that the yield and radical scavenging activity of neutral extract with EtOAc were higher than that of acid and basic extract. After shaken at 30 degrees C, 100 r/min for 5 days, this strain produced largest amount of neutral extract. Further partitioning the neutral fraction with silica gel column chromatograph, LH-20 column chromatograph, MPLC and HPLC, we screened 15 free radical scavengers whose yield was more than 2 mg and purity was higher than 85% in HPLC (260 nm absorption). Several of them were analyzed with 1H-NMR and 13C-NMR spectrum from which we concluded that B1-3 and C3-1-7 were two kinds of substitute of benzene, and C3-1-7 may be 3-OH-4-OCH3-benzoic acid. The scavenging activity of 40 mumol/L B1-3 was about 65%, and that of C3-1-7 was lower than 56%, while as the control the scavenging activity of 40 mumol/L Vc and Ve was 80.4% and 78.4% respectively.
Wu
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College of Life Sciences, Zhejiang University, Hangzhou 310012.
Wu
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D001555:Benzene Derivatives / Q000737:chemistry / Q000302:isolation & purification; D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D006834:Hydrazines; D032741:Monascus / Q000378:metabolism*; D010851:Picrates
D001555:Benzene Derivatives; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006834:Hydrazines; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl