[The comparative study on ova superovulated, in vitro fertilization and rate of pregnancy at different ages].
The development of oocytes, superovulated at 28, 56 and 112 days in three mice strains (ICR, B6C3F1, and C57BL/6J) with PMSG and HCG, were examined using the techniques of in vitro fertilization, culture, and transfer of these two-cell embryos to pseudopregnant recipients. The highest numbers of oocytes were obtained from superovulated 28-day-old mice in three strains. Approximately 90% of oocytes developed to the two-cell stage after in vitro fertilization, and about 50% became pregnant through the recipients. These results suggested that donor age at 28 days had prominent discrepancy with 56 and 112-day-old mice (P < 0.01) in oocytes superovulation, and no influence on the rate of insemination and pregnancy.
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Shanghai Laboratory Animals Center, Chinese Academy of Sciences & National Laboratory Animal Resources, Shanghai Branch, Shanghai 200233.
D000367:Age Factors; D000818:Animals; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D011247:Pregnancy; D018873:Pregnancy Rate; D013480:Superovulation