[Conditions of enzyme production and properties of alkaline lipase by Streptomyces Z94-2].
An alkaline lipase producing strain streptomyces Z94-2 was selected from 152 lipase producer, The optimal medium composition for the producing lipase is (g/L): soybean meal 30, urea 10, dextrin 10, KH2PO4 0.5, NaCl 1, MgSO4.7H2O 0.5, AEO9 (nonionic surfactant) 1. The optimal conditions for lipase producing are initial pH 9.5-10.0, shaken at 26 degrees C, for 48 h, a maximal lipase activity of reached 596 (u/mL). The optimal activity at pH 9.8 and at 37 degrees C, respectively. The lipase was stable at pH 8.6-10.2 and below 60 degrees C, and the lipase was highly activated by 0.14 mol/L CaCl2.
Zhou
X
X
Department of Biology Engineering, Zhejiang University of Technology, HangZhou 310014.
Huang
J
J
Ou
Z
Z
Wang
H
H
Wang
R
R
D002122:Calcium Chloride; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000096:biosynthesis / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology*; D013696:Temperature
D003470:Culture Media; D008049:Lipase; D002122:Calcium Chloride