title: Overlapping deletions define novel embryonic lethal loci in the mouse t complex.
abstract: The t complex region of mouse chromosome 17 contains genetic information critical for embryonic development. To identify and map loci required for normal embryogenesis, a set of overlapping deletions (D17Aus9(df10J), D17Aus9(df12J), and D17Aus9(df13J)) surrounding the D17Aus9 locus and one encompassing the T locus, Del(17)T(7J), were bred in various combinations and the consequences of nullizygosity in overlapping regions were examined. The results indicated that there are at least two functional units within 1 cM of D17Aus9. l17J1 is a peri-implantation lethal mutation within the region deleted in D17Aus9(df13J), whereas l17J2 is a later-acting lethal defined by the region of overlap between Del(17)T(7J) and D17Aus9(df12J). Del(17)T(7J)/D17Aus9(df12J) embryos die around 10.5 dpc. The development of the mutant embryos is characterized by lack of axial rotation, an abnormal notochord structure, and a ballooning pericardium. These studies demonstrate the value of overlapping deletion complexes, as opposed to individual deletion complexes, for the identification, mapping, and analysis of genes required for embryonic development.
authors.affiliation: Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6445, USA.
authors.forename: Hanna H J
authors.initials: HH
authors.lastname: Chao
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Mentzer
authors.forename: John C
authors.initials: JC
authors.lastname: Schimenti
authors.forename: Yun
authors.initials: Y
authors.lastname: You
mesh_terms: D000483:Alleles; D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D003433:Crosses, Genetic; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D017403:In Situ Hybridization; D051379:Mice; D008957:Models, Genetic; D009154:Mutation; D009672:Notochord / Q000378:metabolism; D013997:Time Factors