Insertion of cellular sequence and RNA recombination in the structural protein coding region of cytopathogenic bovine viral diarrhoea virus.
The cytopathogenic bovine viral diarrhoea virus (cp BVDV) strain KS86-1cp was isolated from a calf persistently infected with the noncytopathogenic (ncp) strain KS86-1ncp after it was exposed to cp BVDV strain Nose and developed mucosal disease (MD). Molecular analysis revealed that an insertion of a cellular gene and a duplication of the viral RNA encoding the nucleocapsid protein C and part of N(pro) had occurred in the C coding region of the Nose and KS86-1cp genomes. The inserted cellular gene was closely related to the cINS sequence. Remarkably, the 5' upstream region from the insertion of KS86-1cp had high sequence identity to that of Nose, but differed from that of KS86-1ncp. In contrast, the region downstream from the insertion of KS86-1cp showed high identity to KS86-1ncp, but not to Nose. These data reveal that KS86-1cp is a chimeric virus generated by homologous RNA recombination in a calf with MD.
Nagai
Makoto
M
Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa 920-3101, Japan.
Sakoda
Yoshihiro
Y
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Mori
Masashi
M
Laboratory of Plant Molecular Genetics Research Institute of Agricultural Resources, Ishikawa Agricultural College, Ishikawa 921-8836, Japan.
Hayashi
Michiko
M
Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa 920-3101, Japan.
Kida
Hiroshi
H
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Akashi
Hiroomi
H
National Institute of Animal Health, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000821:virology; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics*
D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D015678:Viral Structural Proteins