[Cloning and expression of VEGF receptor Flt-1 gene in S. lividans TK24].
Using RNA extracted from human umbilical vein endothelium cell as a template, the gene VEGF receptor Flt-1 was amplified by RT-PCR. Recombinant plasmid pSGLgpp-F was constructed and was transformed into S. lividans TK24. With the detection of SDS-PAGE and Western blot, a specific band being same to the reports near 63.6 kd was found. The results showed sFlt-1 was successfully expressed in S. lividans. The result of the binding assay of receptor-ligand for sFlt-1 showed sFlt-1 has the biological activity of binding with its ligand VEGF.
Jiang
Zibin
Z
Institute of Medicinal Biotechnology, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100050, China.
Li
Yuan
Y
D003001:Cloning, Molecular; D042783:Endothelial Cells / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*; D014169:Transformation, Bacterial; D014471:Umbilical Veins / Q000737:chemistry; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification
D011994:Recombinant Proteins; D040281:Vascular Endothelial Growth Factor Receptor-1