Neuroprotective effects of R(-)-1-(benzo[b]thiophen-5-yl)-2- [2-(N,N-diethylamino)ethoxy]ethanol hydrochloride (T-588) against retinal ganglion cell death induced by elevated intraocular pressure in rat.
OBJECTIVE
We investigated whether T-588 can attenuate retinal ganglion cell (RGC) death induced by elevated intraocular pressure (IOP).


METHODS
IOP elevation was induced unilaterally by argon laser irradiation of the rat trabecular meshwork 4 days after an intracameral injection of India ink. We orally administered either the vehicle, or 10, 30, or 100 mg/kg body weight (BW) of T-588 24 hours before the laser application. Five days after the laser application, 1.5 microL of 3% Fast Blue was injected into the superior colliculi bilaterally. Three days after the Fast Blue injection, the eye was enucleated and the retinal whole flatmount was prepared. Labeled ganglion cells were counted by fluorescence microscope with an ultraviolet filter.


RESULTS
Laser treatment significantly increased the IOP. The percentages of labeled RGCs in the lasered eyes as compared with the nonlasered contralateral eyes were 78.0 +/- 11.6% in the control group, 78.7 +/- 12.9% in the 10 mg/kg BW group, 79.1 +/- 13.0% in the 30 mg/kg BW group, and 91.0 +/- 9.0% in the 100 mg/kg BW T-588-treated group. The survival rate of RGCs was significantly higher in the 100 mg/kg BW T-588-treated group than in the control group.


CONCLUSIONS
T-588 appears to have a neuroprotective effect on retinal ganglion cells in this ocular hypertensive model.
Karim
Md Zahidul
MZ
Department of Ophthalmology, Gifu University School of Medicine, Gifu, Japan.
Gu
Zhaobin
Z
Sawada
Akira
A
Taniguchi
Toru
T
Kawase
Kazuhide
K
Yamamoto
Tetsuya
T
Kitazawa
Yoshiaki
Y
D000284:Administration, Oral; D000818:Animals; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D004048:Diethylamines / Q000494:pharmacology*; D007429:Intraocular Pressure; D053685:Laser Therapy; D008297:Male; D008856:Microscopy, Fluorescence; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009798:Ocular Hypertension / Q000150:complications; D009901:Optic Nerve Diseases / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000473:pathology; D013876:Thiophenes / Q000494:pharmacology*; D014129:Trabecular Meshwork / Q000601:surgery
D004048:Diethylamines; D018696:Neuroprotective Agents; D013876:Thiophenes; C082372:T 588