[Gene regulation studies on purine biosynthetic in Salmonella typhimurium IX. Mutation analysis of PUR box 2].
Two consensus bases C and G in 16 bp PUR box were directed mutated to G and A separately by PCR amplification. The binding function of PUR box carrying mutation with PUR protein were examined by gel retardation experiment. The results showed that the PUR box with above mutations could not bind with purR protein extracted from LT2. It proved that the two consensus bases C and G are necessary for PUR box binding with purR protein.
Ma
W
W
Department of Biochemistry, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093.
Qin
J
J
Wang
A
A
D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D011687:Purines / Q000096:biosynthesis*; D012097:Repressor Proteins / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C065825:PurR protein, Bacteria; D011687:Purines; D012097:Repressor Proteins; C030985:purine