cDNA cloning and developmental expression of murine carbonic anhydrase-related proteins VIII, X, and XI.
To help understand the biological functions of carbonic anhydrase-related proteins (CA-RPs VIII, X, and XI), we obtained cDNA clones of murine CA-RPs X and XI and studied the tissue distribution of all three CA-RPs and their developmental expression in the murine embryonic brain. The amino acid sequences of murine CA-RPs are highly conserved with their human homologues, indicating a fundamental biological role of CA-RPs. Among a panel of vital organs, the strongest mRNA expression of all three CA-RPs was consistently observed in the brain. Immunohistochemical analysis revealed the expression of all three CA-RPs in the neural cell body and neurites. CA-RP X was also observed in the myelin sheath, as demonstrated using the shiverer demyelinated mouse. In murine embryos, CA-RP VIII and X messages first appeared in the middle of the gestation period, while the CA-RP XI message was seen at an early gestational stage and expressed to a lesser extent as gestation progressed. All CA-RPs were expressed in the neuroprogenitor cells in the subventricular area and subsequently detected in the neural cells migrating to the cortex. Although the exact function of CA-RPs is still undefined, these findings suggest an important role of CA-RPs in the CNS.
Taniuchi
Keisuke
K
First Department of Internal Medicine, Kochi Medical School, Nankoku, Kochi 783-8505, Japan.
Nishimori
Isao
I
Takeuchi
Tamotsu
T
Ohtsuki
Yuji
Y
Onishi
Saburo
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D014408:Biomarkers, Tumor; D001921:Brain / Q000033:anatomy & histology / Q000201:enzymology; D002256:Carbonic Anhydrases; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000201:enzymology; D011247:Pregnancy; D014018:Tissue Distribution; D047908:Intracellular Signaling Peptides and Proteins
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D002256:Carbonic Anhydrases; D018076:DNA, Complementary; D007527:Isoenzymes; D009419:Nerve Tissue Proteins; C512815:CA10 protein, human; C512817:CA11 protein, human; C512819:Car10 protein, mouse; C512820:Car11 protein, mouse; C512821:Car8 protein, mouse; C512818:CA8 protein, human; D047908:Intracellular Signaling Peptides and Proteins