[Determination of solanesol in the extracts of tobacco leaves by high performance liquid chromatography].
A method for the determination of solanesol in the extracts of tobacco leaves by high performance liquid chromatography has been developed. The column used was Shim-pack CLC-SIL(5 microns, 5 mm i.d. x 150 mm) and the UV detector was set at 215 nm. The eluent was a mixture of n-hexane and isopropyl alcohol (98:2, V/V). As the results showed, the linear range was 1 microgram-10 micrograms(r = 0.9997), the average recovery of solanesol was 98.1% and the relative standard deviation was 1.9%. This method is fast, accurate and reproducible.
Zhang
M S
MS
Center for Analysis and Test, Guizhou Normal University, Guiyang 550001, China. zhangmingshi@263.net
Huang
J X
JX
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D013729:Terpenes / Q000032:analysis*; D014026:Nicotiana / Q000737:chemistry*
D013729:Terpenes; C017719:solanesol