[Studies on the simultaneous measurement of several cephalosporins by RP-HPLC (I)].
This paper reported the research on the simultaneous separation and determination of six cephalosporins by RP-HPLC. Six cephalosporins are cefalcor, cefalexin, cefradine, cefadroxil, cefominox and cefoxitin. The analytical conditions for this method were as follows: a Hypersil ODS C18(200 mm x 4.6 mm i.d., 5 microns), detection wavelength: 254 nm; a mobile phase solution of 50 mmol/L monopotassium phosphate (pH 3.4)-acetonitrile (87.5:12.5) and DAD detector. The flow rate was 1.0 mL/min. The calibration curves of the six compounds were linear, the correlation coefficients were 0.9951 for cefominox, 0.9999 for the others, the range were 164 ng-16.4 micrograms for cefominox, 99 ng-9.934 micrograms for cefadroxil, 104 ng-10.358 micrograms for cefalcor, 122 ng-12.224 micrograms for cefalexin, 107 ng-10.702 micrograms for cefradine and 115 ng-11.506 micrograms for cefoxitin. The recovery rates were 103.5% for cefominox, 99.3% for cefadroxil, 101.4% for cefalcor, 101.5% for cefalexin, 98.7% for cefradine and 97.6% for cefoxitin. Six cephalosporins were all stable in 50 mmol/L monopotassium phosphate (pH 3.4-4.6). When preparations of these cephalosporins were determined, it is indicated there were no difference between the results by using this method and the pharmacopoeia methods. The total separation time of these cephalosporins was within fifteen minutes. This method is simple, sensitive, rapid and accurate.
Wu
Z J
ZJ
Xiamen Institute for Drug Control, Xiamen 361012, China.
Guo
W B
WB
Zhang
Q G
QG
Ni
K Y
KY
Lin
Y S
YS
D000900:Anti-Bacterial Agents / Q000032:analysis; D002433:Cefaclor / Q000032:analysis; D002434:Cefadroxil / Q000032:analysis; D002506:Cephalexin / Q000032:analysis; D002511:Cephalosporins / Q000032:analysis*; D002515:Cephradine / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D002434:Cefadroxil; D002433:Cefaclor; D002515:Cephradine; D002506:Cephalexin