3'-Ferrocene-labeled oligonucleotide chains end-tethered to gold electrode surfaces: novel model systems for exploring flexibility of short DNA using cyclic voltammetry.
The flexibility of DNA is of central importance in biology, medicine, materials science, and mechanical engineering. In this study, we report an unprecedented electrochemical approach for investigating the flexibility of a short (typically 20-base), surface end-tethered single-stranded synthetic DNA oligonucleotide and of its postformed DNA duplex, taking as an example the homopolymer (dT)20 sequence in the regime of very high ionic strength ( approximately 1 M).
Anne
Agnès
A
Laboratoire d'Electrochimie Moléculaire, Unité Mixte de Recherche Université-CNRS No 7591, Université de Paris 7 - Denis Diderot, 2 place Jussieu, 75251 Paris Cedex 05, France. anne@paris7.jussieu.fr
Bouchardon
Agnès
A
Moiroux
Jacques
J
D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000031:analogs & derivatives; D018076:DNA, Complementary / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D000075163:Metallocenes; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*
D018076:DNA, Complementary; D004277:DNA, Single-Stranded; D004220:Disulfides; D005296:Ferrous Compounds; D000075163:Metallocenes; D009841:Oligonucleotides; D006046:Gold; D003545:Cysteine; C004998:ferrocene