[The effect of culture conditions on the fatty acid composition in the mycelium of the Cephalosporium sp].
The optimal condition of Cephalosporium sp. to culture mycelia and accumulate polyunsaturated fatty acid (PUFA) was researched. The optimal culture condition to get mycelium productivity was: maltose 60 g/L, KNO3 3 g/L, initial pH 6.0, 100 mL medium in 500 mL flask, seeding 25% (v/v), 25 degrees C culture it for 10 days. The optimal condition to accumulation PUFA proportion to total fatty acid was: glucose 10-20 g/L, NH4Cl or (NH4)2SO4 3 g/L, initial pH 4.0, 100 mL medium in 500 mL falsk, seeding 10-20% (v/v) and lighted it when it was cultured. It was suggested that two step could be used in the producing progress. A proposal was put forward that the oileic acid transformed to linoleic acid was the key step to produce PUFA. This proposal gave a base to research the optimal culture condition and enzyme regulation.
Dai
C
C
Biology Science College of Nanjing Normal University, Nanjing 210097, China.
Yuan
S
S
Li
X
X
Liu
J
J
Xing
D
D
Lu
L
L
D000164:Acremonium / Q000737:chemistry* / Q000187:drug effects / Q000254:growth & development; D003470:Culture Media; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D008027:Light; D008320:Maltose / Q000494:pharmacology*; D025282:Mycelium / Q000737:chemistry* / Q000187:drug effects / Q000254:growth & development; D009566:Nitrates / Q000494:pharmacology; D017680:Potassium Compounds / Q000494:pharmacology; D013696:Temperature
D003470:Culture Media; D005231:Fatty Acids, Unsaturated; D009566:Nitrates; D017680:Potassium Compounds; D008320:Maltose; C023844:potassium nitrate