CIP353 encodes an AP2/ERF-domain protein in potato (Solanum tuberosum L.) and responds slowly to cold stress.
We isolated the CIP353 cDNA, which encodes a novel cold-inducible protein, from cold-stored tubers of potato (Solanum tuberosum L.). The level of CIP353 transcripts began to increase in tubers 2 weeks after storage at 3 degrees C and continued increasing for at least 3 months during storage. This increase was not observed in tubers stored at >or=9 degrees C. The increased level of transcripts in tubers stored at 3 or 6 degrees C decreased when the tubers were shifted to 20 degrees C. These data suggest that CIP353 is a temperature-dependent and slowly responsive cold-inducible gene of potato. The middle of the deduced amino acid sequence of CIP353 cDNA showed high similarity to the AP2/ERF domain, which occurs in some plant regulatory factors. The deduced protein contained a putative basic nuclear-localization signal and potential acidic activation domains. These data suggest that CIP353 protein is a transcription factor of genes expressed in tubers under long-term storage at low temperatures.
Mine
Toshiki
T
Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc., 700 Higashibara, Toyoda, Iwata, Shizuoka, 438-0802 Japan.
Hiyoshi
Toru
T
Kasaoka
Keisuke
K
Ohyama
Akio
A
D000064:Acclimatization / Q000235:genetics* / Q000502:physiology; D000595:Amino Acid Sequence; D001483:Base Sequence; D003080:Cold Temperature; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018547:Plant Stems / Q000235:genetics / Q000502:physiology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic / Q000235:genetics
D018076:DNA, Complementary; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D010940:Plant Proteins