title: Modeled channel distributions explain extracellular recordings from cultured neurons sealed to microelectrodes.
abstract: Amplitudes and shapes of extracellular recordings from single neurons cultured on a substrate embedded microelectrode depend not only on the volume conducting properties of the neuron-electrode interface, but might also depend on the distribution of voltage-sensitive channels over the neuronal membrane. In this paper, finite-element modeling is used to quantify the effect of these channel distributions on the neuron-electrode contact. Slight accumulation or depletion of voltage-sensitive channels in the sealing membrane of the neuron results in various shapes and amplitudes of simulated extracellular recordings. However, estimation of channel-specific accumulation factors from extracellular recordings can be obstructed by co-occuring ion currents and defect sealing. Experimental data from cultured neuron-electrode interfaces suggest depletion of sodium channels and accumulation of potassium channels.
authors.affiliation: Institute for Biomedical Technology, Signals and Systems Group, Faculty of Electrical Engineering, University of Twente. PO Box 217, 7500 AE Enschede, The Netherlands. J.R.Buitenweg@el.utwente.nl
authors.forename: Jan Reinoud
authors.initials: JR
authors.lastname: Buitenweg
authors.forename: Wim L C
authors.initials: WL
authors.lastname: Rutten
authors.forename: Enrico
authors.initials: E
authors.lastname: Marani
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D002448:Cell Adhesion; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D003198:Computer Simulation; D004574:Electromagnetic Fields; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D005110:Extracellular Space / Q000502:physiology*; D020342:Finite Element Analysis; D005727:Ganglia, Spinal / Q000502:physiology; D015640:Ion Channel Gating / Q000502:physiology*; D007473:Ion Channels / Q000502:physiology; D008839:Microelectrodes; D008959:Models, Neurological; D009474:Neurons / Q000502:physiology*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015222:Sodium Channels / Q000502:physiology*
chemical_list: D020099:Coated Materials, Biocompatible; D007473:Ion Channels; D015222:Sodium Channels