[Immunofluorescence studies on the localization of integrin-like protein in plant pollen tubes].
The Strong fluorescence signals were obtained in pollen tube of Lilium davidii Duch with labeled anti-VnR integrin serum, and anti-beta 3, alpha v integrin subunit cytoplasmic domain serum separately. The highest density of immunolabel was in the tip of pollen tube. There was little or no immunolabel in control experiment using non-immune serum, second antibody alone and anti-FnR, LnR integrin serum separately. In pollen of Prunus persica f. rubro-piena Schneid, fluoresence signals were also obtained in tube using labeled anti-beta 1, beta 3 integrin subunit cytoplasmic domain serum separately and in apertures using anti-beta 1 serum. Preliminary results show that during the germination of pollen and the growth of pollen tube, there may be integrin-like proteins in pollen tube, consisting of alpha v and beta s integria subunits in the pollen tube of Lilium davidii Duch, which is the receptor of vitronectin-like protein.
Sun
Y
Y
Department of Biology, Hebei Normal University, Shijiazhuang 050016.
Mu
R L
RL
Hao
M P
MP
Sun
D Y
DY
Zhang
Z H
ZH
D005109:Extracellular Matrix / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D016023:Integrins / Q000032:analysis*; D027762:Lilium / Q000737:chemistry*; D011058:Pollen / Q000737:chemistry; D019030:Receptors, Vitronectin / Q000276:immunology
D016023:Integrins; D019030:Receptors, Vitronectin