[Construction of recombinant antisense RNA IgG2aVH region of murine systemic lupus erythematous].
OBJECTIVE
To construct recombinant plasmid of IgG2aVH region antisense RNA of systemic lupus erythematous.


METHODS
Total RNA was isolated from spleen cell of BWF1 mice. Using spleen cell total RNA as the template, We designed specific primers from A6.1 region sequences, and amplified the IgG2aVH region 375 bp DNA fragments by RT-PCR. The IgG2aVH cDNA was cloned by T/A and inserted into pcDNA 3.1 plasmid of vector.


RESULTS
The IgG2aVH antisense RNA plasmid expressing recombinant was identified by restriction enzyme digestion and DNA sequence analysis.


CONCLUSIONS
There is IgG2aVH gene in F1 mice spleen cell; the IgG2aVH antisense RNA expressing recombinants is constructed successfully.
Deng
J W
JW
Center Research Laboratory, Second Xiangya Hospital, Central South University, Changsha, 410011, China.
Lu
Q J
QJ
Hu
T T
TT
D000818:Animals; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000235:genetics; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008814:Mice, Inbred NZB; D010957:Plasmids / Q000235:genetics; D016372:RNA, Antisense / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D007074:Immunoglobulin G; D016372:RNA, Antisense; D011994:Recombinant Proteins