[Characterization of expression of the apoptosis associated PF18-3 molecule in thymocyte apoptosis following ConA activation].
The expression pattern of McAb PF18-3 recognized molecule in activation induced thymocyte apoptosis was analysed. Results indicated that following activation by ConA, thymocytes underwent activation induced apoptosis identified by delayed production of DNA ladder and TUNEL positive staining of thymocytes in late stage of activation. PF18-3 molecule was found to express specifically in subset of apoptotic thymocytes with diploid. Kinetic comparison of expression between PF18-3 molecule and Fas or translocated membrane phospholipid suggested that PF18-3 molecule is different from them and likely to be a novel molecule related to thymocyte apoptosis.
Wang
H
H
Key Lab of Medical Immunology, Department of Immunology, Beijing Medical University, Beijing 100083.
Xiao
S Y
SY
Pang
X W
XW
Chen
W F
WF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D017209:Apoptosis; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D013950:Thymus Gland / Q000166:cytology*
D000911:Antibodies, Monoclonal; D003208:Concanavalin A