[Isolation, purification and identification of metallthionein from strain BD 101 of Saccharomyces cerevisiae].
Selection of Saccharomyces cerevisiae BD101 having resistance to metal ions and producing metallothionein by inducer from Saccharomyces. These proteins were isolated and purified by Sephadex G-50 and subsequent DEAE Sepharose CL-4B, then Sephadex G-25 for desalination. There were two iso-MTs for Cu-induced. The, molecular weights were 7 kD and had 60 amino acids. Both Cu-MTs were rich in Cysteine (10%). 4 atom Cu/6 mole Cys/mole Cu-MTs.
Lin
Z
Z
College of Life Sciences, Peking University, Beijing 100871.
Chang
L
L
D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008667:Metalloproteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D000596:Amino Acids; D008667:Metalloproteins