title: Crystallization and preliminary X-ray analysis of TBP-interacting protein from the hyperthermophilic archaeon Thermococcus kodakaraensis strain KOD1.
abstract: The 26 kDa TBP (TATA-binding protein) interacting protein from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 (Tk-TIP26) is a possible transcriptional regulatory protein in Thermococcales. Here, the crystallization of both the native and selenomethionine-substituted proteins and data collection are described. The native crystals belong to the tetragonal space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = 73.83, c = 86.41 A, and diffract to 2.2 A using synchrotron radiation. MAD data was collected and a Bijvoet difference Patterson map showed strong peaks sufficient to determine the positions of the Se atoms.
authors.affiliation: Department of Materials Chemistry, Graduate School of Engineering, Osaka University, Yamada-oka 2-1, Suita, Osaka 565-0871, Japan.
authors.forename: Takahiko
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Tomoki
authors.initials: T
authors.lastname: Matsuda
authors.forename: Nami
authors.initials: N
authors.lastname: Sakamoto
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Masaaki
authors.initials: M
authors.lastname: Morikawa
authors.forename: Shigenori
authors.initials: S
authors.lastname: Kanaya
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kai
mesh_terms: D019843:Archaeal Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D012645:Selenomethionine / Q000737:chemistry; D017356:Synchrotrons; D035181:TATA-Box Binding Protein / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D019712:Thermococcus / Q000737:chemistry* / Q000235:genetics
chemical_list: D019843:Archaeal Proteins; D035181:TATA-Box Binding Protein; D012645:Selenomethionine