Antioxidant effect of polyamines on erythrocyte cell membrane lipoperoxidation after free-radical damage.
This in vitro study determined the effect of three doses each (100, 500 and 1000 microm) of putrescine, spermidine and spermine on malondialdehyde (MDA) release in red blood cells (RBCs) from healthy individuals after hydrogen peroxide stimulation (10 mM). Twenty-two volunteers, 9 males and 13 females, aged 41.5 +/- 16.4 years, were studied. MDA was measured by thiobarbituric reaction (TBARs) and the results were calculated using epsilon = 1.56 x 10(5). The analysis of variance (ANOVA) demonstrated a statistically significant overall decrease in MDA release in the polyamine-exposed cells (p < 0.0001) when compared with unexposed cells. Individual analysis of each polyamine separately showed a 52% decrease in MDA release with added spermine and a 39.5% decrease with added spermidine (p < 0.001). No evaluable effect was found for putrescine. There was no correlation between the effect produced and the three doses of spermidine or spermine added, indicating a non dose-dependent action.
Farriol
Mireia
M
Centro de Investigación Bioquímica y Biologia Molecular (CIBBIM), Hospital Vall d'Hebron, Barcelona, Spain. farriol@hg.vhebron.es
Segovia-Silvestre
Toni
T
Venereo
Yusimy
Y
Orta
Xavi
X
D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D008875:Middle Aged; D008517:Phytotherapy; D011700:Putrescine / Q000008:administration & dosage / Q000494:pharmacology*; D013095:Spermidine / Q000008:administration & dosage / Q000494:pharmacology; D013096:Spermine / Q000008:administration & dosage / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D013096:Spermine; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D013095:Spermidine; D011700:Putrescine