[The effects of reactive oxidants on Bacillus thuringiensis parasporal crystals].
The effects of hydrogen peroxides(H2O2) and hydroxyl radicals(.OH) on the purified parasporal crystals from Bacillus thuringiensis var. Kurstaki HD-1 were studied by SDS-PAGE and bioassay. The results showed that to some extent H2O2 and .OH produced a direct and deleterious effect. Hydroxyl radicals from the system Fe(II)-EDTA-H2O2 indicated more serious damage than hydrogen peroxides. There was a 44.5% loss in activity against 12-h-old larvae of Heliothis armigera when parasporal crystals were treated with 0.3% H2O2 only. However, 85.2% activity lost when parasporal crystals were treated with 0.3% H2O2 in the presence of iron(II) and EDTA.
Wang
W
W
Department of Applied Chemistry, China Agricultural University, Beijing 100094.
Qian
C
C
Shen
J
J
Yang
S
S
D000818:Animals; D001413:Bacillus thuringiensis / Q000187:drug effects / Q000472:pathogenicity*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000187:drug effects*; D001427:Bacterial Toxins; D004731:Endotoxins / Q000737:chemistry; D006460:Hemolysin Proteins; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000494:pharmacology*; D007814:Larva / Q000382:microbiology; D007915:Lepidoptera / Q000382:microbiology
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide