title: The effect of cycloheximide on butyrylcholinesterase activity in vivo.
abstract: The paper describes the catalytic activity of butyrylcholinesterase (BuChE) measured in plasma, liver, white adipose tissue, heart, and brain of rats intraperitoneally administered a single non-lethal dose of cycloheximide (2.0 mg/kg body weight; CHM). The BuChE assay was performed on rats of both sexes either administered CHM or saline (controls), and killed 2, 3, 4, 5, 10 days later. A significant decrease of BuChE catalytic activity was observed in all tested tissues except plasma. In animals of both sexes, the lowest BuChE catalytic activity was found in the liver (2-6%), while it was higher in white adipose tissue, heart, and brain. However, the respective values remained significantly different from controls (33-67%, 49-62%, and 14-71% in males, and 24-82%, 72-86%, and 33-67% in females). Since there was no effect of CHM on BuChE catalytic activity in plasma, the data suggest that CHM inhibits the synthesis of BuChE rather than its active site.
authors.affiliation: Institute for Medical Research and Occupational Health, Zagreb, Croatia. Ana.Lucic@imi.hr
authors.forename: Ana
authors.initials: A
authors.lastname: Lucić
authors.forename: Vlasta
authors.initials: V
authors.lastname: Bradamante
authors.forename: Bozica
authors.initials: B
authors.lastname: Radić
authors.forename: Maja
authors.initials: M
authors.lastname: Peraica
authors.forename: Ana-Marija
authors.initials: AM
authors.lastname: Domijan
authors.forename: Radovan
authors.initials: R
authors.lastname: Fuchs
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002091:Butyrylcholinesterase / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D003513:Cycloheximide; D002091:Butyrylcholinesterase