Studies on clonogenic hemopoietic cells of vertebrate in space: problems and perspectives.
Hemopoietic tissues were studied in vertebrates launched aboard the Soviet (Russian) biosatellites ("Cosmos-1129, 1514, 1667, 1887 and 2044"; "Bion-10 and 11") between 1980 and 1996. In the bone marrow of rats exposed to spaceflight conditions, a statistically significant decrease in cell number was revealed in the progenitor cell compartment accounting for the compensatory response of granulocyte-macrophage (CFU-gm) and erythrocyte lineages (BFU-e and CFU-e) and in the compartment of multipotent hemopoietic stem cells (CFU-s), which is responsible for the permanent renewal of hemopoietic tissue. The number of stromal fibroblastic progenitors (CFC-f) in the bone marrow of these rats was also reduced. Apparently, changes in the hemopoietic stroma damage the hemopoietic microenvironment and, hence, may be responsible for changes observed in the hemopoietic tissue proper. Attempts were made to develop methods for analyzing morphologically indiscernible clonogenic hemopoietic cells of newts, and studies on the effects of spaceflight factors on these cells were performed. The results showed that the numbers of clonogenic cells in newts of the flight group newts were significantly lower than in control newts. The data obtained are used as the basis for formulating the problems to be studied, drawing up a program for further research on the effects of spaceflight factors on stem and other clonogenic hemopoietic cells, and developing new experimental models for analyzing stem cells, the state of the hemopoietic stroma, etc.
Domaratskaya
E I
EI
Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
Michurina
T V
TV
Bueverova
E I
EI
Bragina
E V
EV
Nikonova
T A
TA
Starostin
V I
VI
Khrushchov
N G
NG
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000378:metabolism*; D005260:Female; D006098:Granulocytes; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D008099:Liver / Q000166:cytology; D008297:Male; D010999:Pleurodeles / Q000097:blood / Q000502:physiology; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013026:Space Flight; D013154:Spleen / Q000166:cytology; D017154:Stromal Cells; D014893:Weightlessness
D003115:Colony-Stimulating Factors