[Studies on hydantoinase producing conditions of Arthrobacter K1108].
The hydantoinase-producing conditions from strain Arthrobacter K1108 were investigated. It is shown that the hydantoinase in the strain is intracellular and inducible. Its optimal inducer is 5-benzylhydantoin, while 5-indolylmethylhydantoin and 5-phenylhydantoin cannot induce the production of hydantoinase in K1108. A gratuitous inducer was designed, with which the hydantoinase production is 343% as much as that with 5-benzylhydantoin. The media for culturing the bacteria were screened and optimized. Under optimal conditions, the specific activity of K1108 cells reached 10.8 U/mL.
Zhang
Weicai
W
Institute of Biotechnology, Beijing, 100071, China. zhangwc@nic.bmi.ac.cn
Hao
Shufeng
S
Yuan
Hongjie
H
Wang
Shujin
S
Huang
Liuyu
L
D000581:Amidohydrolases / Q000096:biosynthesis*; D001173:Arthrobacter / Q000166:cytology / Q000201:enzymology*; D018929:Cell Culture Techniques; D003470:Culture Media; D006827:Hydantoins / Q000494:pharmacology*
D003470:Culture Media; D006827:Hydantoins; C067244:5-indolylmethylhydantoin; C031427:5-benzylhydantoin; C098839:5-phenylhydantoin; D000581:Amidohydrolases; C020047:dihydropyrimidinase