[Expression of the artificial cecropin CMIV-like gene of Chinese silkworm Bombyx mori in Laphygma exigua larvae].
The synthesized CMIV-like Gene was linked with the signal peptide gene of nature silkworm antibacterial peptide and was inserted into baculovirus expression vector pFastBac 1, construcing a recombinant transposing vector. The vector was transformed into DH10Bac competent E. coli cells. The recombinant Bacmid was obtained. The recombinant Bacmid was transfected into sf21 cells to get the recombinant virus. Laphygma exigua larvae were infected with the recombinant virus to express the antibacterial peptide. The hemolymph were tested to have antibacterial activity. The active antibacterial peptide was purified by acid polyacrylamide electrophoresis. The specific expression of mRNA of CMIV-like Gene was tested using Northern blotting.
Zhao
D
D
College of Life Sciences, Nanjing Normal University, Nanjing 210097, China.
Dai
Z
Z
Zhou
K
K
Zhang
L
L
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D012831:Bombyx / Q000737:chemistry; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism; D017344:Genes, Insect; D005813:Genes, Synthetic; D019476:Insect Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D007814:Larva / Q000235:genetics / Q000821:virology; D009036:Moths / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D011994:Recombinant Proteins / Q000235:genetics; D018411:Spodoptera / Q000166:cytology / Q000378:metabolism
D019476:Insect Proteins; D011994:Recombinant Proteins; C405946:cecropin CMIV protein, Bombyx mori