[The study of ethanol-water used as reversed-phase high performance liquid chromatographic mobile phase].
The viscosity of ethanol-water at various temperatures was determined. The physicochemical properties and characters of the solvents used as the mobile phase of reversed-phase high performance liquid chromatography(RP-HPLC) were compared between methanol and ethanol. The components of several Chinese medicines have been determined by RP-HPLC with ethanol-water used as mobile phase, and the results were compared with those obtained with methanol-water or acetonitrile-water. It showed that ethanol can be used as RP-HPLC mobile phase by increasing the column temperature moderately.
Fu
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School of Pharmacy, West China University of Medical Science, Chengdu 610041, China.
Li
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Liu
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Chen
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D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D000431:Ethanol; D005419:Flavonoids / Q000032:analysis; D007529:Isoflavones / Q000032:analysis; D007773:Lactates / Q000032:analysis; D000432:Methanol; D014867:Water
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D007529:Isoflavones; D007773:Lactates; D014867:Water; C038044:baicalin; D000431:Ethanol; C035055:3,4-dihydroxyphenyllactic acid; D000432:Methanol; C033607:puerarin