Identification and characterization of Mlr1,2: two mouse homologues of Mblk-1, a transcription factor from the honeybee brain(1).
We previously identified the Mblk-1 gene in the honeybee brain, which encodes a transcription factor containing two DNA binding motifs, termed RHF1 and 2 (Takeuchi et al. (2001) Insect Mol. Biol. 121, 134-140). Here, we identified two mouse Mblk1 homologues, Mlr1 and Mlr2. Both encode proteins containing a single DNA-binding motif highly conserved with RHF2 and activate transcription mediated by a DNA element recognized by honeybee Mblk-1. Mlr1 was expressed predominantly in the spermatocytes of the testis, while Mlr2 was expressed in various tissues other than testis. Mlr1 transcripts were lost in the testis of W/W(v) mutant mice, suggesting a role in spermatogenesis.
Kunieda
Takekazu
T
Bio-oriented Technology Research Advancement Institution (BRAIN), 3-18-19, Toranomon, Minato-ku, Tokyo 105-0001, Japan.
Park
Jung-Min
JM
Takeuchi
Hideaki
H
Kubo
Takeo
T
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001516:Bees; D001923:Brain Chemistry; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D019476:Insect Proteins / Q000235:genetics*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis; D020218:Response Elements / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D013090:Spermatocytes / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*
D018076:DNA, Complementary; D019476:Insect Proteins; C471289:Mlr1 protein, mouse; C471290:Mlr2 protein, mouse; D012333:RNA, Messenger; D014157:Transcription Factors