Anticellular and immunosuppressive properties of ethanolic extract of Acorus calamus rhizome.
Modulation of immune response to alleviate disease has been of interest since long. Plant extracts have been widely investigated for possible immunomodulatory properties. We have evaluated the anticellular and immunomodulatory properties of ethanolic extract of Acorus calamus rhizome. This extract inhibited proliferation of mitogen (phytohaemagglutinin; PHA) and antigen (purified protein derivative; PPD)-stimulated human peripheral blood mononuclear cells (PBMCs). In addition, A. calamus extract inhibited growth of several cell lines of mouse and human origin. It also inhibited production of nitric oxide (NO), interleukin-2 (IL-2) and tumor necrosis factor-alpha (TNF-alpha). Intracytoplasmic interferon-gamma (IFN-gamma) and expression of cell surface markers, CD16 and HLA-DR, on human PBMC, were not affected on treatment with A. calamus extract but CD25 expression was down regulated. Our study demonstrates the antiproliferative and immunosuppressive potential of ethanolic extract of A. calamus rhizome in vitro.
Mehrotra
S
S
Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow 226 014, India.
Mishra
K P
KP
Maurya
R
R
Srimal
R C
RC
Yadav
V S
VS
Pandey
R
R
Singh
V K
VK
D029023:Acorus / Q000737:chemistry*; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D010835:Phytohemagglutinins / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry*; D014373:Tuberculin / Q000494:pharmacology
D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; C063419:CTAGE1 protein, human; D016207:Cytokines; D006684:HLA-DR Antigens; D007166:Immunosuppressive Agents; D008562:Membrane Glycoproteins; D010835:Phytohemagglutinins; D010936:Plant Extracts; D014373:Tuberculin; D000431:Ethanol