[Immobilization of penicilin G acylase on polyacrylonitrile fiber].
The immobilization of Penicillin G Acylase from Bacillus megaterium by glutaraldehyde crosslinking on the partially acid-hydrolyed polyacrylonitrile fiber was studied. When the amount of--NH2 on fiber were 690 mumol/g and the moisture in the fiber was 64%, and the content of enzyme protein immobilized on fiber was more than 100 mg/g. The activity of 2300 IU/g was obtained with 30% of overall yield and 56% of binding efficiency. The immobilization yield was markedly influenced by ratio of the amount of free enzyme used to the weight of the fiber. The half-life of storage stability of immobilized PGA at room temperature was 130 days. The immobilized PGA kept 80% of the initial activity after 20 cycles of operation in 10% of PGK(W/V) in 0.05 mol/L phosphate buffer, pH 8.0, at 37 degrees C and an enzyme load of 150 IU/g(PGK) and 10 g(PGK) for per cycle of operation. The hydrolysis conversion of PGK in the range of 2.5%-12.5% (W/V) were over 98% for the immobilized PGA. The operation stability of immobilized PGA treated with DTT was better than that of immobilized PGA untreated.
Xian
H
H
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Wang
Z
Z
D000180:Acrylic Resins / Q000737:chemistry*; D001410:Bacillus megaterium / Q000737:chemistry / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D010399:Penicillin Amidase / Q000737:chemistry* / Q000378:metabolism
D000180:Acrylic Resins; D004800:Enzymes, Immobilized; C010504:polyacrylonitrile; D010399:Penicillin Amidase