title: Lipoarabinomannan-reactive human secretory immunoglobulin A responses induced by mucosal bacille Calmette-Guérin vaccination.
abstract: The ability of 17 recombinant mycobacterial proteins, native antigen 85 complex, lipoarabinomannan (LAM), and Mycobacterium tuberculosis lysate to detect antibody responses induced by bacille Calmette-Guérin (BCG) vaccination and active tuberculosis infection were studied in enzyme-linked immunosorbent assays. Only LAM-reactive serum immunoglobulin G responses were significantly increased in both BCG-vaccinated patients and patients with active tuberculosis (P<.05), and oral BCG vaccination also induced significant increases in LAM-reactive secretory immunoglobulin A (P<.05). LAM-reactive antibody assays can serve as markers of humoral and mucosal immunity in future trials of BCG and newer attenuated mycobacterial vaccines.
authors.affiliation: Saint Louis University Vaccine and Treatment Evaluation Unit, Division of Infectious Diseases and Immunology, Department of Internal Medicine, Saint Louis University, St. Louis, Missouri 63110, USA.
authors.forename: Robin M
authors.initials: RM
authors.lastname: Brown
authors.forename: Orlando
authors.initials: O
authors.lastname: Cruz
authors.forename: Michael
authors.initials: M
authors.lastname: Brennan
authors.forename: Maria L
authors.initials: ML
authors.lastname: Gennaro
authors.forename: Larry
authors.initials: L
authors.lastname: Schlesinger
authors.forename: Yasir A W
authors.initials: YA
authors.lastname: Skeiky
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Hoft
mesh_terms: D000293:Adolescent; D000328:Adult; D001500:BCG Vaccine / Q000276:immunology*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D014611:Vaccination
chemical_list: D001500:BCG Vaccine; D007070:Immunoglobulin A; D008070:Lipopolysaccharides; C050016:lipoarabinomannan