title: The human herpesvirus 6 U100 gene product is the third component of the gH-gL glycoprotein complex on the viral envelope.
abstract: The human herpesvirus 6 (HHV-6) variant A U100 gene encodes the third component of the glycoprotein H (gH)-glycoprotein L (gL)-containing complex. Glycosidase digestion analysis showed that the U100 gene products are glycoproteins consisting of an 80-kDa protein with complex N-linked oligosaccharides and a 74-kDa protein with immature, high-mannose N-linked oligosaccharides. Based on these characteristics, we designated the U100 gene products glycoprotein Q (gQ). Only the 80-kDa form of gQ was coimmunoprecipitated with an anti-gH antibody, suggesting that the 80-kDa protein associates with the gH-gL complex in HHV-6-infected cells. Furthermore, the complex was detected in purified virions, suggesting that it may play an important role in viral entry.
authors.affiliation: Department of Microbiology, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan. ymori@micro.med.osaka-u.ac.jp
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Mori
authors.forename: Pilailuk
authors.initials: P
authors.lastname: Akkapaiboon
authors.forename: Xuwei
authors.initials: X
authors.lastname: Yang
authors.forename: Koichi
authors.initials: K
authors.lastname: Yamanishi
mesh_terms: D002478:Cells, Cultured; D005312:Fetal Blood; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D006801:Humans; D015151:Immunoblotting; D011233:Precipitin Tests; D013601:T-Lymphocytes / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: C077406:UL115 protein, Human herpesvirus 5; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C071440:glycoprotein H, Cytomegalovirus; C499358:glycoprotein H, Human cytomegalovirus