Crystallization and preliminary X-ray crystallographic analysis of plant ferritin from Glycine max.
The iron storage protein ferritin from soybean (Glycine max) was expressed in E. coli and crystallized using the hanging drop vapor diffusion method with sodium tartrate as the precipitant. The crystals belong to the tetragonal I4(1)22 space group, with unit cell parameters a=b=324.0, c=182.7 A. The diffraction data were collected up to a resolution of 3.0 A with a multi-wire area detector.
Masuda
Taro
T
Department of Bio-Science, Central Research Institute of Electric Power Industry, 1646 Abiko, Abiko-shi, 270-1194, Chiba, Japan. t-masuda@riepi-denken.or.jp
Mikami
Bunzo
B
Goto
Fumiyuki
F
Yoshihara
Toshihiro
T
Utsumi
Shigeru
S
D003460:Crystallization; D005293:Ferritins / Q000737:chemistry* / Q000302:isolation & purification; D010940:Plant Proteins / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*; D014961:X-Ray Diffraction
D010940:Plant Proteins; D005293:Ferritins