[Determination of acyclovir in mouse plasma and tissues by reversed-phase high performance liquid chromatography].
The aim of this study was to establish an high performance liquid chromatographic method for determining acyclovir (ACV) concentration in mouse plasma and tissues. A solution of 0.25 mL 60 g/L perchloric acid and 0.25 mL acetonitrile was added into 0.2 mL plasma or 0.2 g tissues to precipitate proteins. Following centrifugation, the supernatant obtained was injected into a reversed-phase column. Operating conditions were Hypersil ODS column(250 mm x 4.6 mm i.d., 5 microns), methanol-water-acetic acid(1:99:0.5, volume ratio) solution as mobile phase at a flow rate of 1.5 mL/min, UV detection at 252 nm. The detection limit of ACV concentration in plasma was 20 micrograms/L and that in tissues was 50 ng/g. The standard curves for ACV were linear in plasma and homogenate of tissues (r > 0.99). The precision of the method was good and the recoveries of ACV were higher than 97.5%. So this method is rapid, accurate and convenient for determination of ACV concentrations in plasma and tissues.
Xu
Y
Y
Department of Clinical Pharmacology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. XuJY@Xinhuanet.com
Zhou
S W
SW
Tang
J L
JL
Huang
L Q
LQ
D000212:Acyclovir / Q000032:analysis / Q000097:blood*; D000818:Animals; D000998:Antiviral Agents / Q000032:analysis / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008099:Liver / Q000737:chemistry*; D051379:Mice
D000998:Antiviral Agents; D000212:Acyclovir