Cytotoxicity and mutagenesis induced by singlet oxygen in wild type and DNA repair deficient Escherichia coli strains.
Singlet oxygen ((1)O(2)) is a product of several biological processes and can be generated in photodynamic therapy, through a photosensitization type II mechanism. (1)O(2) is able to interact with lipids, proteins and DNA, leading to cell killing and mutagenesis, and can be directly involved with degenerative processes such as cancer and aging. In this work, we analyzed the cytotoxicity and mutagenesis induced after direct treatment of wild type and the DNA repair fpg and/or mutY deficient Escherichia coli strains with disodium 3,3'-(1,4-naphthylidene) diproprionate endoperoxide (NDPO(2)), which releases (1)O(2) by thermodissociation. The treatment induced cell killing and mutagenesis in all strains, but the mutY strain showed to be more sensitive. These results indicate that even (1)O(2) generated outside bacterial cells may lead to DNA damage that could be repaired by pathways that employ MutY protein. As (1)O(2) is highly reactive, its interaction with cell membranes may generate secondary products that could react with DNA, leading to mutagenic lesions.
Cavalcante
Ana Karina Dias
AK
Departamento de Biologia Celular e Genética, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal CEP 59072970, RN, Brazil.
Martinez
Glaucia Regina
GR
Di Mascio
Paolo
P
Menck
Carlos Frederico Martins
CF
Agnez-Lima
Lucymara Fassarella
LF
D004249:DNA Damage; D045647:DNA Glycosylases; D004260:DNA Repair / Q000235:genetics; D004269:DNA, Bacterial / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D043382:DNA-Formamidopyrimidine Glycosylase; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D016296:Mutagenesis; D009699:N-Glycosyl Hydrolases / Q000235:genetics / Q000378:metabolism; D026082:Singlet Oxygen / Q000633:toxicity
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D026082:Singlet Oxygen; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; C061738:mutY adenine glycosylase; D043382:DNA-Formamidopyrimidine Glycosylase; C468610:DNA-formamidopyrimidine glycosylase, E coli