title: [Study of deletion and mutation of p16 gene in primary hepatocellular carcinoma].
abstract: OBJECTIVE
  To investigate the role the deletion and mutation of p16 gene plays in the pathogenesis of human primary hepatocarcinoma.
  
  
  METHODS
  Thirty-one cases of human hepatocarcinoma, 31 cases of adjacent noncancerous liver cirrhosis and the leukocytes of 8 normal human subjects were analyzed for deletion and mutation in p16 gene exons 1, 2 and introns 1, 2 with comparative multiple PCR and PCR-SSCP.
  
  
  RESULTS
  Deletion of p16 gene exon 1 and partial intron 1 was found in 4 of 31 cases (13/%). No deletion of exon 2 or intron 2 was found. Three patterns of p16 gene intron 1 and 18 bp-flanking sequence in exon 2 at SSCP analysis were observed in hepatocellular carcinoma and corresponding adjacent noncancerous cirrhosis, and two patterns were found in human normal leukocyte DNA. No aberrant single strand at SSCP in p16 gene exon 1 or most part of exon 2 or intron 2 was detected.
  
  
  CONCLUSIONS
  Low frequency of deletion and rare mutation of p16 suppressor gene occurred in hepatocellular carcinoma.
authors.affiliation: Laboratory of Molecular Biology, WCUMS, Chengdu 610041, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Qin
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.forename: Z
authors.initials: Z
authors.lastname: Sun
authors.forename: F
authors.initials: F
authors.lastname: Zuo
authors.forename: Z
authors.initials: Z
authors.lastname: Sun
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis* / Q000235:genetics; D005091:Exons; D017353:Gene Deletion; D019942:Genes, p16; D006801:Humans; D007438:Introns; D008113:Liver Neoplasms / Q000235:genetics*; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16