[Effects of immune bovine whey on sucrose-dependent adherence of Streptococcus mutans].
OBJECTIVE
The aim of this experiment is to explore the possible anti-carious mechanisms of immune bovine whey.


METHODS
The immune milk was collected from cows immunized with cell associated glucosyltransferase(GFT) over-expression strain B-29-33 of Streptococcus mutans(S. mutans) MT8148. The control milk was obtained from non-immunized cows. The immune and control bovine whey were prepared by removing fat and casein. The effects of two kinds of bovine whey on sucrose-dependent adherence of S. mutans MT8148 were examined by using TTY media with five different volumes of the immune and the control milk, 100, 300, 500, 700 and 900 microliters respectively. The adherent rates were determined by reading the optical density. The adherent morphology was observed by using a scanning electron microscope(SEM).


RESULTS
There was no significant difference in adherence of S. mutans MT8148 cells to the smooth glass surface among five doses with those of the control whey. The immune bovine whey inhibited the adherence of S. mutans. The adherent percentage was 84.37% at 100 microliters and decreased by 58.59% at 900 microliters. The inhibition was does-dependent. The difference of adherent morphology between the immune and control whey was noticeable at 900 microliters. The SEM revealed that cells of S. mutans MT8148 with the control whey were covered by large quantities of gelatinous material, but cells with the immune whey were clearly distinguished.


CONCLUSIONS
The immune whey has inhibitory potential against the sucrose-dependent adherence of S. mutans.
Fan
M
M
College of Stomatology, Hubei Medical University.
Chen
W
W
Bian
Z
Z
D000818:Animals; D000907:Antibodies, Bacterial / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D002417:Cattle; D003731:Dental Caries / Q000382:microbiology; D004305:Dose-Response Relationship, Drug; D008855:Microscopy, Electron, Scanning; D008892:Milk / Q000276:immunology*; D013295:Streptococcus mutans / Q000187:drug effects* / Q000648:ultrastructure; D013395:Sucrose / Q000276:immunology
D000907:Antibodies, Bacterial; D013395:Sucrose