[Determination of beta-carotene in tobacco by HPTLC].
This paper reports the high performance thin-layer chromatography (HPTLC) determination of beta-carotene in tobacco. The developer used was V(benzene):V(acetone) = 4:1. The detection wavelength was 450 nm. The linear range and the detectable limit of the method were 50 ng-0.5 microgram and 20 ng per spot of beta-carotene respectively. The method has been proved to be simple, sensitive, accurate and reproducible.
Shi
L
L
Hefei Institute of Economics and Technology, Hefei 230052, China.
Zhu
X L
XL
Liu
B Z
BZ
Gao
Y
Y
D002855:Chromatography, Thin Layer / Q000379:methods*; D014026:Nicotiana / Q000737:chemistry*; D019207:beta Carotene / Q000032:analysis*
D019207:beta Carotene