[Construction and expression of somatostatin (S14) and hepatitis B surface antigen gene in yeast Pichia pastoris].
To construct the ss/HBsAg protein gene-engineering vaccine for developing the diagnosis and cure tumors in clinical medicine and promoting the growth in animal husbandry production. A pair of primers were designed according separately to the sequence of Somatostatin gene(S14) and HBsAg gene. Their gene fragments were separately amplified by using PCR and cloned, following sequencing, the DNA fragments were inserted into pBluescript vector. Then the ss/HBsAg chimera was constructed and was cloned into pPICZaA plasmid, and transformed into electroporated Pichia pastoris. High yield protein expression was obtained. Expressed protein was proved with high specificity and it's molecular weigh was about 28 KD identified by SDS-PAGE and Western blot.
Wang
Zezhou
Z
College of Life Science, Sichuan University, Sichuan Provincial Station of Animal Epidemic Prevention and Control, Chengdu 610041.
Gao
Rong
R
Yang
Lin
L
Liu
Shigui
S
D019677:Artificial Gene Fusion; D003001:Cloning, Molecular; D015870:Gene Expression; D005822:Genetic Vectors; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D010843:Pichia / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D013004:Somatostatin / Q000235:genetics*
D006514:Hepatitis B Surface Antigens; D011993:Recombinant Fusion Proteins; D013004:Somatostatin