[Changes of CGRP activity and distribution in facial nucleus after facial nerve injury of guinea pig].
OBJECTIVE
In order to study the changes of CGRP activity in facial nucleus of guinea pig after facial nerve injury.


METHODS
The activity of CGRP was detected by the technique of immunohistochemical and image analysis methods were used.


RESULTS
It was showed the there were CGRP-immunoreactivity (IR) in normal facial nucleus and its' subnucleus. An initial increase of CGRP-IR was noted and the content of CGRP under image analysis were significantly different when compared with control group (P < 0.01) at day 1 after injury of facial nerve. CGRP continued to rise to a maximal level at day 7 (P < 0.001). And after which it gradually decreased. We noticed distinct bundle-shaped CGRP fibers elongated to the peripheral part from ventral facial nucleus in the period of 14 d-35 d.


CONCLUSIONS
It suggests that during the course of facial nerve regeneration following injury, CGRP changed characteristically. CGRP may play the regulational role in facial nerve regeneration.
Ma
H
H
Department of Otolaryngology, Bethune International Peace Hospital, Shijiazhuang 050082.
Wang
J
J
Qiu
J
J
Huang
W
W
Jiang
H
H
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D005154:Facial Nerve / Q000473:pathology / Q000502:physiology; D020220:Facial Nerve Injuries / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D008297:Male; D009046:Motor Neurons / Q000378:metabolism*; D009416:Nerve Regeneration
D015740:Calcitonin Gene-Related Peptide