[Construction of genomic library from L. lactis AL2 and isolation of entire nisin biosynthesis gene cluster].
A total DNA library of Lactococcus lactis AL2 with high yield of nisin was successfully constructed using lambda EMBL3 as vector. 3900 plaques were obtained, which is much more than the required number of recombinants that represents an entire L. lactis genome according to Clarke and Carbon formula. Southern hybridization, PCR amplification and DNA sequencing revealed that the entire nisin biosynthesis gene cluster was isolated from the constructed library.
Chen
X
X
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
Hu
H
H
Jia
S
S
Chen
M
M
Huan
L
L
D001483:Base Sequence; D015139:Blotting, Southern; D015723:Gene Library; D005822:Genetic Vectors; D013294:Lactococcus lactis / Q000235:genetics*; D008969:Molecular Sequence Data; D009561:Nisin / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction
D009561:Nisin