Attenuation by spinal cord stimulation of a nociceptive reflex generated by colorectal distention in a rat model.
The mechanisms underlying the cause and treatment of visceral pain of gastrointestinal origin are poorly understood. Previous clinical studies have shown that spinal cord stimulation (SCS) attenuates neuropathic and ischemic pain, and animal experiments have provided knowledge about probable physiological mechanisms. The goal of the present study was to investigate whether SCS influences colonic sensitivity in a conscious rat. A visceromotor behavioral response (VMR), induced by colorectal distention, was used to quantify the level of colonic sensitivity. Under anesthesia, an electrode (cathode) was placed on the dorsal surface of the spinal cord at L1. One week after implantation of the SCS electrode, the effects of stimulation delivered with different intensities (50 Hz, 0.2 ms for 30 min) on colonic sensitivity were determined. Nociceptive levels of colorectal distention (60 mm Hg for 10 min) induced an enhanced VMR quantified as an increased number of abdominal muscle contractions compared to controls in which the balloon catheter was inserted into the colorectal region but not distended. Colonic sensitization with acetic acid increased the VMR to innocuous levels of colorectal distention (30 mm Hg for 10 min). We found that SCS induced a significant depression of the VMR produced by colorectal distention in both normal rats and those with sensitized colons. The suppressive effect of SCS on colonic sensitivity suggests that SCS may have therapeutic potential for the treatment of visceral pain of gastrointestinal origin associated with abdominal cramping and painful abdominal spasms.
Greenwood-Van Meerveld
Beverley
B
Oklahoma Foundation for Digestive Research, Basic Science Laboratories, Veterans Affairs Medical Center, Research Administration Rm. 151, 921 N.E. 13th St., Oklahoma City, OK 73104, USA. Beverley-Greenwood@ouhsc.edu
Johnson
Anthony C
AC
Foreman
Robert D
RD
Linderoth
Bengt
B
D000818:Animals; D003106:Colon / Q000502:physiology*; D004599:Electric Stimulation Therapy / Q000379:methods*; D004567:Electrodes, Implanted; D008297:Male; D010147:Pain Measurement / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012007:Rectum / Q000502:physiology*; D013116:Spinal Cord / Q000502:physiology*; D017833:Visceral Afferents / Q000502:physiology