title: Protein tyrosine phosphatase PTP20 induces actin cytoskeleton reorganization by dephosphorylating p190 RhoGAP in rat ovarian granulosa cells stimulated with follicle-stimulating hormone.
abstract: We identified 25 protein tyrosine phosphatases (PTPs) expressed in rat ovarian granulosa cells. Of these PTPs, the expression levels of at least PTP20, PTP-MEG1, PTPepsilonM, and PTPepsilonC significantly changed during the estrous cycle. We examined the cellular functions of PTP20 in granulosa cells by expressing the wild type, a catalytically inactive CS mutant in which Cys229 of PTP20 was changed to Ser, or a substrate-trapping DA mutant in which Asp197 was mutated to Ala, using an adenovirus vector. Overexpression of the wild type, but not of the CS mutant, induced retraction of the cell body with the extension of long, dendritic-like processes after stimulation with FSH, a critical factor for the survival and differentiation of these cells. In addition, cell adhesion to the substratum decreased in an FSH-dependent manner. Inhibiting Rho GTPase activity with C3 botulinum toxin caused similar morphological changes. The FSH-enhanced phosphotyrosine (p-Tyr) level of p190 RhoGAP was selectively reduced by the overexpressed wild type, but not by mutated PTP20. Although p190 RhoGAP is tyrosine phosphorylated by c-Src via the tyrosine kinase Pyk2, wild-type PTP20 had little effect on p-Tyr418 of c-Src and no effect on p-Tyr402 of Pyk2, which are required for full c-Src activity and for interacting between Pyk2 and c-Src, respectively. The CS and DA mutants as well as the wild type reduced the formation of p190 RhoGAP-p120 RasGAP complexes. Confocal microscopy analysis revealed that PTP20 intracellularly colocalizes with p190 RhoGAP. These results demonstrate that PTP20 regulates the functions of granulosa cells in an FSH-dependent manner by dephosphorylating p190 RhoGAP and subsequently inducing reorganization of the actin cytoskeleton. Moreover, our data suggest that PTPs play significant roles in controlling the dynamics of ovarian functions.
authors.affiliation: Gene Research Center, University of Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shiota
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tanihiro
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Naohito
authors.initials: N
authors.lastname: Aoki
authors.forename: Norio
authors.initials: N
authors.lastname: Ishida
authors.forename: Koyomi
authors.initials: K
authors.lastname: Miyazaki
authors.forename: Axel
authors.initials: A
authors.lastname: Ullrich
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Miyazaki
mesh_terms: D000199:Actins / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001905:Botulinum Toxins / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D004268:DNA-Binding Proteins; D030762:Estrous Cycle / Q000502:physiology; D005260:Female; D051418:Focal Adhesion Kinase 2; D005640:Follicle Stimulating Hormone / Q000378:metabolism / Q000494:pharmacology*; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D020662:Guanine Nucleotide Exchange Factors / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D010053:Ovary / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation; D017027:Protein Tyrosine Phosphatases / Q000187:drug effects / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D054558:Protein Tyrosine Phosphatases, Non-Receptor; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012097:Repressor Proteins; D013997:Time Factors; D014443:Tyrosine / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; C513040:Arhgap35 protein, rat; D004268:DNA-Binding Proteins; D020662:Guanine Nucleotide Exchange Factors; D009687:Nuclear Proteins; D012097:Repressor Proteins; D014443:Tyrosine; D005640:Follicle Stimulating Hormone; D011505:Protein-Tyrosine Kinases; D051418:Focal Adhesion Kinase 2; D016392:Proto-Oncogene Proteins pp60(c-src); C496054:Ptk2b protein, rat; D017027:Protein Tyrosine Phosphatases; D054558:Protein Tyrosine Phosphatases, Non-Receptor; C481950:Ptpn18 protein, rat; D001616:beta-Galactosidase; D001905:Botulinum Toxins; C096324:botulinum toxin type C