[Cloning and sequencing of cDNA from G protein gene of subgroup B respiratory syncytial virus strain isolated in China].
The nucleotide sequence of the G protein gene of respiratory syncytial virus (RSV) CC169 strain isolated from China that has been identified as subgroup B with monoclonal antibodies, was determined from cDNA that had been amplified by RT-PCR and cloned into pTZ18R plasmid vector. The homology of nucleotide was 94% as compared with G protein cDNA of a RSV prototype strain (CH18537). Deduced amino acid identity of G protein was 89.4%. The amino acid changes were only in the extracellular part of the protein where there were two extensive divergent domains with a highly conserved region in between; whereas the cytoplasmic and transmembrane domains were conserved. This study demonstrates the sequence diversity of the G protein of subgroup B RSV between a Chinese isolate and the prototype strain CH18537.
Geng
X
X
Beijing Municipal Laboratory of Infection and Immunity, Capital Institute of Pediatrics, Beijing 100020.
Wang
Z
Z
Qian
Y
Y
Zhu
R
R
Deng
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D002648:Child; D018076:DNA, Complementary / Q000032:analysis; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics* / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D014764:Viral Proteins