[Immunohistochemical study of safe resection margin in the surgical treatment of laryngeal carcinoma].
OBJECTIVE
To study safe resection margin of laryngectomy according to the level of protein of gene expression.


METHODS
Using citric-LSAB-immunochemical technique, the expressions of ras, c-myc and P53 were studied in different regions of larynx including 30 cases laryngeal carcinoma, border area, adjacent mucosa which was 0.5, 1.0, 1.5, 2.0 cm away from cancer and four normal laryngeal mucosa.


RESULTS
The results showed that both single expression and co-expression of p21, p62 and p53 increased as tissue progressed in sequence from normal mucosa, 2.0, 1.5, 1.0, 0.5 cm distant mucosa adjacent to carcinoma, border area and carcinoma. A significant different expression in most of them can be seen at regions of 0.5 cm and 1.0 cm distant from the tumor (P < 0.05).


CONCLUSIONS
The tissue 0.5 cm adjacent to the tumor should be regarded as precancerous lesion. It is appropriate to regard 0.5 cm away from tumors as resection margins for surgical treatment of laryngeal carcinoma.
Wan
B
B
Department of Otolaryngology, People's Hospital of Henan Province, Zhengzhou 450003.
Dong
M
M
Ma
J
J
Jia
J
J
Ma
G
G
Xie
W
W
Dong
M
M
D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D011230:Precancerous Conditions / Q000378:metabolism / Q000601:surgery; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
D016271:Proto-Oncogene Proteins c-myc; D016159:Tumor Suppressor Protein p53; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)