High resolution genotyping of Campylobacter jejuni strains by macrorestriction analysis with XhoI and polymerase chain reaction targeting enterobacterial repetitive intergenic consensus sequences: can we predict the zoonotic potential of strains?
Campylobacter jejuni isolates of human, canine, feline, bovine and poultry origin were investigated for their genomic diversity using O-antigen typing (n = 271), SmaI (n = 158) and XhoI (n = 158) macrorestriction analysis and ERIC-PCR (n = 107). The O-antigens O:1/44, O:2, O:4 complex, O:37. O:40 were identified and 53.7% of the human and 56.1% of the animal strains were typable with the available antisera. Two ERIC-PCR pattern groups were generated representing human and animal strains as well as those exclusively of animal origin. XhoI macrorestriction analysis also distinguished 'human' and 'non-human' strain clusters, but by SmaI restriction mainly serotype-associated clusters were found. In conclusion, genomic differences may occur between 'human' and 'non-human' strains and this may reflect their potential to overcome the barrier from animals to humans.
Moser
I
I
Institut für Mikrobiologie und Tierseuchen, Freie Universität, Berlin, Germany.
Lentzsch
P
P
Rieksneuwoehner
B
B
Schwerk
P
P
Wieler
L H
LH
D000818:Animals; D002169:Campylobacter Infections / Q000635:transmission; D016123:Campylobacter jejuni / Q000235:genetics* / Q000472:pathogenicity*; D002415:Cats; D002417:Cattle; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis*; D004285:Dogs; D005544:Forecasting; D006801:Humans; D019081:O Antigens / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011200:Poultry; D015183:Restriction Mapping; D016415:Sequence Alignment; D015047:Zoonoses
D017931:DNA Primers; D004269:DNA, Bacterial; D019081:O Antigens