[Relationship of hR24L gene expression, DNA damage repair and G2/M phase cell cycle arrest with ion radiation].
The hR24L gene ORF was cloned from the total RNA of HeLa cells by RT-PCR method. There is no mutation of the hR24L gene in HeLa cells. Ion radiation significantly increased the expression of hR24L gene, and the sense hR24L enhanced and accelerated the cell cycle arrest at G2/M phase in HeLa cells. Besides, it should seem that the overexpression of hR24L gene could enhance the repair ability of DNA damage induced by ion radiation, and vice versa.
Yu
T X
TX
Guangdong Provincial Key Laboratory of Marine Pharmacy, Zhanjian Ocean Uriversity, Zhanjian 524025, China.
Zhu
Y B
YB
Tong
T J
TJ
D004249:DNA Damage / Q000528:radiation effects; D004260:DNA Repair / Q000235:genetics*; D004271:DNA, Fungal / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D016195:G2 Phase / Q000528:radiation effects*; D015870:Gene Expression; D005800:Genes, Fungal; D006367:HeLa Cells; D006801:Humans; D011839:Radiation, Ionizing
D004271:DNA, Fungal