Effects of free fatty acids on glucose uptake and utilization in healthy women.
To study effects of sex on free fatty acid (FFA)-induced insulin resistance, we have examined the effects of acute elevations of plasma FFA levels on insulin-stimulated total body glucose uptake in nine healthy young women. Euglycemic-hyperinsulinemic (approximately 500 pmol/l) clamps were performed for 4 h with coinfusion of either lipid/heparin (L/H) to acutely raise plasma FFA levels (from approximately 600 to approximately 1,200 micro mol/l) or saline/glycerol to lower fatty acids (from approximately 600 to approximately 50 micro mol/l). L/H infusion inhibited insulin-stimulated glucose uptake (determined with [3-(3)H]glucose) and glycogen synthesis by 31 and 40%, respectively (P < 0.01), almost completely abolished insulin suppression of endogenous glucose production (EGP) (13.6 vs. 10.0 micro mol x kg(-1) x min(-1), NS), prevented the insulin induced increase in carbohydrate oxidation (8.1 vs. 7.4 micro mol x kg(-1) x min(-1), NS), and stimulated fat oxidation (from 3.6 to 5.1 micro mol x kg(-1) x min(-1), P < 0.01). These data showed that acute increases in plasma FFA levels inhibited the actions of insulin on glucose uptake, glycogen synthesis, and EGP in women to a degree similar to that previously reported in men. We conclude that at insulin and FFA levels in the postprandial range, women and men were susceptible to FFA-induced peripheral and hepatic insulin resistance.
Homko
Carol J
CJ
Division of Endocrinology/Diabetes/Metabolism and the General Clinical Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania, USA.
Cheung
Peter
P
Boden
Guenther
G
D000273:Adipose Tissue / Q000033:anatomy & histology; D000328:Adult; D044383:Black People; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism*; D001824:Body Constitution; D015992:Body Mass Index; D001835:Body Weight; D002153:Calorimetry, Indirect; D004655:Emulsions; D005217:Fat Emulsions, Intravenous / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000097:blood* / Q000494:pharmacology*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D015309:Glucose Clamp Technique; D006003:Glycogen / Q000096:biosynthesis; D006019:Glycolysis / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D006946:Hyperinsulinism / Q000097:blood; D007328:Insulin / Q000008:administration & dosage / Q000494:pharmacology; D054709:Lecithins; D015143:Philadelphia; D012016:Reference Values; D012450:Safflower Oil; D013024:Soybean Oil; D044465:White People; D001741:Black or African American
D001786:Blood Glucose; D004655:Emulsions; D005217:Fat Emulsions, Intravenous; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D054709:Lecithins; C031435:safflower oil, soybean oil, lecithin emulsion; D013024:Soybean Oil; D012450:Safflower Oil; D006493:Heparin; D006003:Glycogen; D005947:Glucose