Glutamine improves impaired cellular exudation and polymorphonuclear neutrophil phagocytosis induced by total parenteral nutrition after glycogen-induced murine peritonitis.
Clinical and laboratory evidence shows that enteral feeding significantly reduces pneumonia and intra-abdominal abscess formation after celiotomy for severe trauma. Supplementation of total parenteral nutrition (TPN) with glutamine (GLN) supports impaired immunity induced by TPN in several animal and human studies. This work investigates the peritoneal cellular response and polymorphonuclear neutrophil (PMN) bactericidal function after mouse chemical peritonitis after TPN with and without GLN. Thirty-three mice received chow, TPN, or 2% GLN-supplemented TPN (GLN-TPN) for 5 days. All mice then received 2 mL of a 1% glycogen solution intraperitoneally to induce cell exudation, and peritoneal exudative cells (PECs) were recovered 4 h later. Total and differential PEC numbers, as well as PMN phagocytosis, reactive oxygen intermediate production (ROI), CD11b (integrin aM chain) expression, and CD16/32 (Fcgamma II/III receptor) expression were measured. PMN, macrophage, and lymphocyte cell numbers were significantly lower with TPN than with chow or GLN-TPN groups, with no differences between chow and GLN-TPN. TPN significantly lowered peritoneal PMN phagocytosis compared with chow (P < 0.05) and approached significance with GLN-TPN (P = 0.06). There were no significant differences in ROI production or CD11b and CD16/32 expression on peritoneal PMN. GLN supplementation improved the reduction in cell exudation and PMN phagocytosis induced by TPN after chemical peritonitis.
Ikeda
Shigeo
S
Department of Surgery, The University of Wisconsin, Madison, Wisconsin 53792, USA.
Kudsk
Kenneth A
KA
Le
Tho
T
Zarzaur
Ben L
BL
Johnson
Cheryl D
CD
D000818:Animals; D001835:Body Weight; D039481:CD11b Antigen / Q000096:biosynthesis; D002448:Cell Adhesion; D005434:Flow Cytometry; D005978:Glutathione / Q000378:metabolism / Q000494:pharmacology*; D006003:Glycogen / Q000378:metabolism / Q000494:pharmacology*; D007249:Inflammation; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D009504:Neutrophils / Q000378:metabolism* / Q000473:pathology*; D010587:Phagocytosis; D017382:Reactive Oxygen Species; D017452:Receptors, IgG / Q000096:biosynthesis
D039481:CD11b Antigen; D017382:Reactive Oxygen Species; D017452:Receptors, IgG; D006003:Glycogen; D005978:Glutathione