title: [Determination of 7-aminoflunitrazepam, the major metabolite of flunitrazepam in urine by high performance thin-layer chromatography].
abstract: A rapid and highly sensitive method, based on high performance thin-layer chromatography, is described for the qualitative and semiquantitative determination of 7-aminoflunitrazepam, the main metabolite of flunitrazepam in human urine. 7-Aminoflunitrazepam in specimen was extracted by solid phase extraction using GDX-403 porous polymer bead as sorbent and ethyl ether as eluant. Fluorescamine was used as a reagent to produce fluorescent product on the plate. The limit of detection was 5 micrograms/L and the limit of quantitation was 15 micrograms/L for 7-aminoflunitrazepam in urine. The method can be successfully used for measuring 7-aminoflunitrazepam in urine samples of the subjects excreted over a 48 h period after receiving 1 mg flunitrazepam orally. The method is applicable to drug examination for the cases of drug-facilitated robbery and rape.
authors.affiliation: Department of Forensic Medicine, Criminal Police College of China, Shenyang 110035, China.
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Wu
authors.forename: Jia-Yi
authors.initials: JY
authors.lastname: Tan
authors.forename: Yong
authors.initials: Y
authors.lastname: Xia
mesh_terms: D002855:Chromatography, Thin Layer / Q000379:methods*; D005445:Flunitrazepam / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000652:urine*; D005450:Fluorescamine; D006801:Humans; D007202:Indicators and Reagents
chemical_list: D007202:Indicators and Reagents; D005450:Fluorescamine; D005445:Flunitrazepam; C036343:7-aminoflunitrazepam