Differential effects of exercise training on skeletal muscle SERCA gene expression.
BACKGROUND
Exercise training induces significant changes in the performance of skeletal muscle.


OBJECTIVE
To determine whether changes in the gene expression of rat hind-limb muscle sarcoplasmic reticulum Ca2+ -ATPase isoforms (SERCA1a and SERCA2a) in response to either moderate- or high-intensity exercise training underlie the functional remodeling.


METHODS
SERCA1a and SERCA2a isoform mRNA expression was determined in predominantly fast-twitch, gastrocnemius muscle and predominantly slow-twitch, soleus muscle with northern analysis. SERCA mRNA was normalized by the 18S rRNA measured in the same sample.


RESULTS
Significant increases in the gastrocnemius SERCA2a mRNA expression were observed after both moderate- and high-intensity training. No significant change in SERCA1a expression was found under any conditions.


CONCLUSIONS
These results indicate that both moderate and high intensity exercise increase the relative SERCA2a expression in the gastrocnemius.
Kubo
Hajime
H
Department of Physiology School of Medicine, College of Health, Physical Education, Recreration and Dance, Temple University, Philadelphia, PA 19140, USA.
Libonati
Joseph R
JR
Kendrick
Zebulon V
ZV
Paolone
Albert
A
Gaughan
Johm P
JP
Houser
Steven R
SR
D000818:Animals; D015152:Blotting, Northern; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D015870:Gene Expression / Q000502:physiology; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases
D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000252:Calcium-Transporting ATPases