Quantitative characterization of differential ion suppression on liquid chromatography/atmospheric pressure ionization mass spectrometric bioanalytical methods.
Ion suppression is a known phenomenon in atmospheric pressure ionization mass spectrometry. These suppression effects have been shown to adversely affect the accuracy and precision of quantitative bioanalytical methods. This paper presents a simple procedure for determining the impact of differential ion suppression on bioanalytical methods that utilize atmospheric pressure ionization mass spectrometric (APIMS) detection. This procedure was applied to the assessment of two potential internal standards, and to determine selectivity issues for another analyte which was to be measured in multiple species.
Avery
Michael J
MJ
Pfizer Global Research and Development, Groton Laboratories, Groton, CT 06340, USA. Michael_j_avery@groton.pfizer.com
D001274:Atmospheric Pressure; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods*; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods*; D012015:Reference Standards; D012680:Sensitivity and Specificity
D007477:Ions