Animal study on expression of laminin and fibronectin in cornea during wound healing following alkali burn.
OBJECTIVE
To observe the expression of laminin and fibronectin in alkali-burned corneas in rats.


METHODS
A total of 18 normal Wistar rats were randomly divided into 6 groups (n = 3 in each group). For each rat, one eye was injured by alkali burn, the other one was taken as the normal control. Then all the corneas were surgically removed and the expression of laminin and fibronectin was observed with immunohistochemistry respectively at 7 hours, 1 day, 3 days, 7 days, 14 days and 28 days after alkali burn.


RESULTS
Compared with that of the normal controls, the expression of laminin and fibronectin of the burned eyes was dramatically higher at 7 hours, reached peak at 14 days and decreased to the normal level at 28 days after alkali burn.


CONCLUSIONS
In the process of wound healing after alkali burn, the expression of laminin and fibronectin increases dramatically, which suggests that laminin and fibronectin may participate in the process of corneal wound healing.
Zhao
Gui-qiu
GQ
Department of Ophthalmology, Affiliated Hospital, Medical College, Qingdao University, Qingdao 266003, China. zhaoguiqui@163.net
Ma
Yi-qun
YQ
Liang
Tao
T
Jiang
Tao
T
Wang
Chuan-fu
CF
Zhang
Yan-xia
YX
D000818:Animals; D002057:Burns, Chemical / Q000378:metabolism*; D065306:Corneal Injuries; D005126:Eye Burns / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D007150:Immunohistochemistry; D007797:Laminin / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000502:physiology*
D005353:Fibronectins; D007797:Laminin