A systematic examination of the in vitro Ussing chamber and the in situ single-pass perfusion model systems in rat ileum permeation of model solutes.
In situ and in vitro intestinal absorption in the rat ileum was systematically studied and mechanistically quantified in terms of permeability coefficients (P) of a series of [(3)H]steroids as model transcellular permeants, [(3)H]taurocholate utilizing the active membrane transport systems to define the aqueous boundary layer (ABL), and [(14)C]urea and [(14)C]mannitol as pore-hindered paracellular diffusants. In situ single-pass perfusion experiments were performed in isolated ileal segments and blood samples were collected from the cannulated mesenteric vein. For the in vitro experiments, an excised, serosal and muscular layer-removed, ileal tissue was mounted in the Ussing chamber diffusion cells. In situ and in vitro P values versus logarithm of the partition coefficient in n-octanol/water (log K) of the steroids were characterized by a sigmoidal-shaped curve in which plateau values were attained for the highly lipophilic steroids with log K greater, similar 2.5. The in situ and in vitro transport barriers in series were viewed as ABL/mucosal epithelium and ABL/mucosal epithelium/submucosal tissue, respectively. Within this framework and the use of experimental strategies and theoretical reasoning, the transport barriers of the steroids were quantitatively delineated and the rate-determining barriers identified. In the plateau region, the analyses indicate that the in situ absorption of the lipophilic steroids was essentially ABL controlled, whereas the in vitro absorption was about equally controlled by diffusion across the ABL and submucosal tissue. The in situ and in vitro pore radii of the paracellular route were 7.2 and 9.2 A, respectively, and the difference was likely the result of perturbation of the tight junctions during the in vitro preparation of the ileal tissue.
Tsutsumi
Keiko
K
Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, 30 South 2000 East, Salt Lake City, Utah 84112, USA.
Li
S Kevin
SK
Ghanem
Abdel-Halim
AH
Ho
Norman F H
NF
Higuchi
William I
WI
D000465:Algorithms; D000818:Animals; D015194:Diffusion Chambers, Culture; D004848:Epithelium / Q000378:metabolism; D007082:Ileum / Q000378:metabolism*; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D007408:Intestinal Absorption / Q000502:physiology; D007700:Kinetics; D008297:Male; D008353:Mannitol / Q000493:pharmacokinetics; D008642:Mesenteric Veins / Q000378:metabolism; D008954:Models, Biological; D010477:Perfusion; D010539:Permeability; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013256:Steroids / Q000493:pharmacokinetics; D013656:Taurocholic Acid / Q000493:pharmacokinetics; D014508:Urea / Q000493:pharmacokinetics
D007202:Indicators and Reagents; D013256:Steroids; D008353:Mannitol; D013656:Taurocholic Acid; D014508:Urea