Analysis of in vitro fertilizing capacity to evaluate the freezing procedures of boar semen and to predict the subsequent fertility.
A porcine in vitro fertilization (IVF) system and seminal quality parameters of frozen-thawed boar semen were used to assess the effectiveness of two different thawing rates of frozen boar semen, and to address the question of whether differences between fertility of ejaculates could be predicted in a limited field trial. In the first experiment, two thawing procedures were analysed (37 degrees C, 30 s; 50 degrees C, 12 s) and no differences in sperm quality were found. However, when the procedure was 50 degrees C, 12 s the IVF results showed a higher number of sperm per penetrated oocyte and a near 10 points higher rate of pronuclear formation. In the second experiment, the fertility results obtained in the limited field trial show to be efficient enough for application in a commercial use, especially for three of the employed boars (fertility > or = 80%). In this limited study, the conventional seminal parameters are not accurate enough to discriminate good and bad boars in relation to fertility. On the contrary, parameters of in vitro penetrability are more precise to predict subsequent fertilities. As conclusion, the IVF fertilization system seems to be a good tool to evaluate the quality of frozen-thawed boar semen previous to its commercial way, to verify the bank semen storage quality and a good way to assay new sperm freezing procedures, as it is the more precise evaluating method in estimating the potential fertilizing ability.
Sellés
E
E
Department of Physiology, Faculty of Veterinary Medicine, University of Murcia, Spain.
Gadea
J
J
Romar
R
R
Matás
C
C
Ruiz
S
S
D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D005260:Female; D005298:Fertility; D005307:Fertilization in Vitro / Q000662:veterinary; D008297:Male; D011247:Pregnancy; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013075:Sperm Capacitation / Q000502:physiology; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013552:Swine / Q000502:physiology*; D013696:Temperature; D013997:Time Factors