[Studies on biotransformation of 5-FUR].
Conditions for biotransformation and purification of FUR were investigated. The result showed that when the cell concentration of E. aerogenes was 10% (w/v), the temperature and pH were 7.8 and 60 degrees C respectively, 59.7% UR was converted to FUR. It also demonstrated that the ideal procedure for the purification of FUR is silica gel column chromatography by two elution systems (S1:CHCl3:CH3OH:H2O = 61:13:1, and S2; CH3COOCH2CH3:CH3OH:CH3COOH:H2O = 12:3:3:2) with the purity and yield of 98.0% and 86.0% respectively.
Ruan
Qiping
Q
School of Biopharmaceutics, China Pharmaceutical University, Nanjing 210009, China.
Zhou
Changlin
C
Dou
Jie
J
Wu
Wutong
W
D001711:Biotransformation; D021902:Enterobacter aerogenes / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D014529:Uridine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism*
C001943:5-fluorouridine; D014529:Uridine