[Production of D-pantolactone hydrolase by Fusarium moniliforme SW-902].
Fusarium moniliforme SW-902, a high-yielding strain for producing D-pantolactone hydrolase was screened. The conditions for enzyme formation of the strain were studied. The suitable carbon source of the medium was glycerol and the suitable nitrogen source was peptone. The optimum temperature and initial pH for enzyme formation were 26 degrees C and pH8.0 respectively. When the organism was cultured in the 60 L-fermentor under the optimum conditions for 45 h, about 7.18 g dry cells/L and 0.92 IU/g dry cells weight were obtained.
Tang
Yixin
Y
School of Biotechnology, Southern Yangtze University, Key Laboratory of Industry Biotechnology, Ministry of Education, Wuxi 214036, China.
Sun
Zhihao
Z
Hua
Lei
L
Guo
Xinfu
X
Wang
Jun
J
D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D005285:Fermentation; D005670:Fusarium / Q000201:enzymology* / Q000302:isolation & purification; D005990:Glycerol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010461:Peptones / Q000378:metabolism; D013696:Temperature
D010461:Peptones; D002265:Carboxylic Ester Hydrolases; C416845:pantoyl lactone hydrolase; D005990:Glycerol