[Assessment of single-stranded DNA breaks in spleen lymphocytes using the DNA comet assay in the long term after acute irradiation of mice at sublethal and moderately-lethal doses].
The single-strand DNA level in spleen lymphocytes of BALB/c male-mice after 11 month acute exposure to gamma-radiation at doses 1, 3 and 6 Gy has been investigated by comet assay. The results of our study showed that at 11 month after irradiation at different doses a significant increase in the level of DNA breaks in spleen lymphocytes and decrease in the total number of these cells in mice was registered. It is possible that the increase in the DNA breaks is due to the effort of the compensatory proliferation process in lymphoid system that can give the increase in the number of different genetic disturbances in lymphocytes.
Miazin
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AE
Moscow State Enterprise-United Ecology-Technology and Science-Industry Center for Decontamination of Radioactive Waste, N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia. and-myazin@yandex.ru
Osipov
A N
AN
Elakov
A L
AL
Sypin
V D
VD
Shevchenko
V A
VA
D000818:Animals; D020552:Comet Assay; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000528:radiation effects* / Q000648:ultrastructure
D004277:DNA, Single-Stranded