[Preparation of latices with functional group containing N and their application in rapid diagnosis of some diseases].
This article reports the preparation and diagnostic application of a variety of latices. These latices, including temary copolymer diazo latex, azidocarbonyl latex and cell labeling latex are stable, monodisperse, soap-free, and covalently combined with protein in the absence of condensing agent. We have improved diazo polystyrene latex and made ternary copolymer diazo latex for early and rapid diagnosis of some diseases. Immunoassay reagent for the dectection of dengue fever has been prepared by our lab using ternary copolymeric diazo latex and azidocarbonyl latex. The experiment showed virus antigen could be detected in 5 mins by using diazo diagnostic agent. The sensitivity of detecting patient serum sample was 82.25%; no-across reaction was observed in other inserted spread virus antigens and in serum of other feverish patient and normal control. 40 serum samples were dectected by latex agglutination assay using ternary copolymeric diazo latex immunoassay reagent. The results were compared with those of PCR and virus separation methods. The sensitivities of the three methods were 82.25%, 75% and 55% respectively; the combined sensitivity was 92.5%; higher sensitivities could be obtained by the former two methods within 1-13 days after the onset of disease.
Wu
D
D
Department of Chemistry, Fourth Military Medical University, Xi'an 710032.
Liu
Y
Y
Zhao
X
X
D001386:Azides; D003715:Dengue / Q000175:diagnosis; D003979:Diazonium Compounds; D006801:Humans; D007840:Latex / Q000737:chemistry*
D001386:Azides; D003979:Diazonium Compounds; D007840:Latex