Purification, crystallization and preliminary X-ray studies of human augmenter of liver regeneration.
Human augmenter of liver regeneration has been expressed in Escherichia coli, purified and crystallized. The crystals belong to space group C222, with unit-cell parameters a=51.7 A, b=78.8 A, c=63.7 A. Diffraction data were collected to 2.80 A with a completeness of 99.9% (99.9% for the last shell), a R(sym) value of 0.092(0.236) and an I/sigma(I) value of 6.2(2.7).
Ji
Chao-Neng
CN
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, 200433, People s Republic of China.
Cai
Zai-Long
ZL
Cao
Gen-Tao
GT
Yin
Gang
G
Jiao
Bing-Hua
BH
Jiang
Tao
T
Shu
Guang
G
Mao
Ji-Fang
JF
Xie
Yi
Y
Mao
Yu-Min
YM
D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D006133:Growth Substances / Q000737:chemistry* / Q000302:isolation & purification*; D006358:Hot Temperature; D006801:Humans; D011506:Proteins; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D050862:Oxidoreductases Acting on Sulfur Group Donors
D006133:Growth Substances; D011506:Proteins; D011994:Recombinant Proteins; C088825:GFER protein, rat; D050862:Oxidoreductases Acting on Sulfur Group Donors