[Gene cloning and expression of human bactericidal/permeability-increasing protein].
The gene of human bactericidal/permeability-increasing protein (BPI) was cloned from peripheral blood lymphocytes of a normal Chinese individual. The result of sequencing showed the gene is 1452 bp encoding a 27-residue signal peptide and a 456-residue matured protein, and it has six nucleotide variations compared with the sequence reported which results in 4 different amino acids. In order to get recombinant BPI, the gene was cloned into an expressing plasmid and expressed in CHO cells. The recombinant protein was purified using cation-exchange chromatography and its bioactivity was proved with bactericidal assays.
Xu
J
J
Institute of Microbiology and Epidemiology, Beijing 100071, China.
Xu
J
J
Wang
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides; D001483:Base Sequence; D001770:Blood Bactericidal Activity; D001798:Blood Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D004926:Escherichia coli / Q000187:drug effects; D015870:Gene Expression; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D017386:Sequence Homology, Amino Acid
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; C021623:bactericidal permeability increasing protein