Purification and properties of a transglutaminase produced by a Bacillus circulans strain isolated from the Amazon environment.
A new microbial transglutaminase (EC 2.3.2.13) from a Bacillus circulans strain isolated from the aquatic Amazonian environment was purified and characterized. Enzyme purification started with (NH(4))(2)SO(4) 'salting out' and proceeded with liquid chromatography on Q-Sepharose FF and octyl-Sepharose 4 FF. The purification factor was approx. 150-fold with a yield of 32%. The enzyme's molecular mass was estimated as 45000 Da on SDS/PAGE. The purified transglutaminase had an optimum temperature of 47 degrees C, the optimum pH of the reaction was 7 and it presented no calcium-dependent activity.
de Barros Soares
Luís Henrique
LH
Universidade Federal do Rio Grande do Sul, Instituto de Ciência e Tecnologia de Alimentos Av Bento Gonçalves, 9500, PO Box 15090, ZC 91501-970, Porto Alegre (RS), Brazil.
Assmann
Francine
F
Záchia Ayub
Marco Antônio
MA
D001407:Bacillus / Q000737:chemistry* / Q000201:enzymology; D019149:Bioreactors; D001938:Brazil; D002118:Calcium / Q000737:chemistry; D002845:Chromatography / Q000379:methods*; D004789:Enzyme Activation; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013045:Species Specificity; D013696:Temperature; D013722:Teprotide; D011503:Transglutaminases / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification*
D013722:Teprotide; D011503:Transglutaminases; D002118:Calcium