[Culture conditions of engineered strain of L-asparaginase and the recombinant plasmid stability].
The growth curves of engineered strain JM105(pASN) were different in LB and M-3 media. The expression level and activity of L-asparaginase were affected apparently by both biomass and induction time. Glucose repression of production of L-asparaginase was found. The stability of the recombinant plasmid pASN in different host strains and in LB and M-3 media was determined. After cultivation inLB broth and M-3 media at 30 degrees C for more than 50 generations without antibiotic selection, then induced at 42 degrees C for up to 5 h, the engineered strains were proved to be stable, except for DHA alpha (pASN).
Wang
Y
Y
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
Qian
S
S
Ye
J
J
Meng
G
G
Zhang
S
S
D001215:Asparaginase / Q000096:biosynthesis / Q000378:metabolism*; D003470:Culture Media; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration
D003470:Culture Media; D004274:DNA, Recombinant; D001215:Asparaginase; D005947:Glucose