Liquid chromatographic separation of the enantiomers of fluoroquinolone antibacterials on a chiral stationary phase based on a chiral crown ether.
A chiral stationary phase (CSP) recently developed by bonding (diphenyl-substituted 1,1'-binaphthyl) crown ether to silica gel for the liquid chromatographic separation of enantiomers was applied to the resolution of investigational fluoroquinolone antibacterial agents including gemifloxacin (formerly LB20304a). All fluoroquinolone compounds used in this study were resolved quite well on the CSP. Especially, the resolution of gemifloxacin and its analogs on the CSP was excellent and even greater than that on the commercial Crownpak CR(+). The resolution was found to be dependent on the type and the content of organic, acidic, and inorganic modifiers added to the mobile phase and on the column temperature.
Hyun
Myung Ho
MH
Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Kuemjeong-Ku, Pusan 609-735, South Korea. mhhyun@hyowon.pusan.ac.kr
Han
Sang Cheol
SC
D000890:Anti-Infective Agents / Q000032:analysis* / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004988:Ethers, Cyclic; D005240:Feasibility Studies; D024841:Fluoroquinolones; D000077735:Gemifloxacin; D009287:Naphthyridines / Q000032:analysis / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*; D013237:Stereoisomerism
D000890:Anti-Infective Agents; D004988:Ethers, Cyclic; D024841:Fluoroquinolones; D009287:Naphthyridines; D012996:Solutions; D000077735:Gemifloxacin