[Antibacterial and antifungal effects of Agkistrodon halys Pallas: purification of its antibacterial protein--LAO].
This paper reports the venom from Agkistrodon halys Pallas have inhibitory activity against fungi and E. coli by tested in a disc diffusion assay. An antibacterial component--LAO from the venom were purified to homogeneous. It had not only antibacterial effect, but L-amino acid oxidase activity. And its enzymatic specific activity was 808 U/mg. The venom had at least 3 antibacterial components (I, II, III) as determined by acid polyacrilamide gel electrophoresis, LAO is the antibaterial components II.
Yan
X M
XM
College of Life Science, Nanjing Normal University, Nanjing 210097.
Zhang
S Q
SQ
Chang
Q
Q
Liu
P
P
Xu
J S
JS
D017836:Agkistrodon; D000594:Amino Acid Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D003435:Crotalid Venoms / Q000201:enzymology / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects*; D005658:Fungi / Q000187:drug effects*; D050830:L-Amino Acid Oxidase
D000890:Anti-Infective Agents; D003435:Crotalid Venoms; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase