Lower apoptosis rate and higher CD69 expression in neutrophils from atopic individuals.
OBJECTIVE
In this study we aimed to examine the kinetics of CD69 expression and the susceptibility to apoptosis, in eosinophils and neutrophils, in the presence or absence of GM-CSF. We also addressed the question whether differences between atopic patients and healthy individuals exist in this respect.


METHODS
Freshly isolated eosinophils and neutrophils from non-atopic and atopic donors were analysed by flow cytometry for Annexin/PI staining, caspase 3 activation and CD69 expression.


RESULTS
We found a higher CD69 expression when atopic neutrophils were incubated with GM-CSF compared to non-atopic neutrophils, and that the kinetics of CD69-expression in neutrophils, but not in eosinophils, differed between non-atopic and atopic individuals (p < 0.004). We also found a higher viability in GM-CSF-stimulated neutrophils from non-atopic individuals as compared to neutrophils from atopic individuals (p < 0.05).


CONCLUSIONS
These data suggest a potential role for neutrophils in the allergic inflammatory reaction through differences in apoptosis rates and CD69 expression between atopic and non-atopic individuals.
Nopp
A
A
Department of Medicine, Karolinska Hospital/Institute, Stockholm, Sweden. Anna.Nopp@ks.se
Stridh
H
H
Grönneberg
R
R
Lundahl
J
J
D017302:Annexins / Q000378:metabolism; D015703:Antigens, CD / Q000096:biosynthesis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis*; D017209:Apoptosis / Q000502:physiology*; D020169:Caspases / Q000378:metabolism; D002469:Cell Separation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004804:Eosinophils / Q000473:pathology / Q000502:physiology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D016179:Granulocyte Colony-Stimulating Factor / Q000502:physiology; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000378:metabolism* / Q000473:pathology*; D066298:In Vitro Techniques; D037181:Lectins, C-Type; D009336:Necrosis; D009504:Neutrophils / Q000473:pathology / Q000502:physiology*; D000099192:CD69 Antigens
D017302:Annexins; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D020169:Caspases; D016179:Granulocyte Colony-Stimulating Factor; D037181:Lectins, C-Type; D000099192:CD69 Antigens