title: Anthocyanins protect against DNA damage induced by tert-butyl-hydroperoxide in rat smooth muscle and hepatoma cells.
abstract: Anthocyanins are flavonoids present in a variety of pigmented food and, like other flavonoids, seem to play a role in preventing human pathologies related to oxidative stress. In fact, anthocyanins have been shown to exert antiproliferative effects in cell cultures and exhibit antiinflammatory and vasoprotective activities in animal models. Although these biological activities have been related to their antioxidant properties, little is known on the molecular mechanism of action of anthocyanins. The effects of pretreatment with the anthocyanins delphinidin, cyanidin, and their glycoside and rutinoside derivatives against induction of DNA damage induced by tert-butyl-hydroperoxide (TBHP) were evaluated in rat smooth muscle and in rat hepatoma cell lines using alkaline single cell gel electrophoresis (Comet test). In addition, a possible protection exerted by anthocyanins on cell killing, lipid peroxidation, and redox state alterations induced by TBHP was also investigated. It was found that the treatment with TBHP induces the formation of DNA single strand breaks (SSB) and oxidised bases, along with cell killing, lipid peroxidation and redox state alteration. Our data demonstrate that anthocyanins are effective against cytotoxicity, DNA SSB formation and lipid peroxidation induced by TBHP, but they do not have any detectable effect against impairment by TBHP of cellular redox state and on protection against DNA bases oxidation. The presence of a sugar moiety in anthocyanin derivatives reduced this protective effect, mainly in rat hepatoma cells. The different activity of anthocyanins and their derivatives may be explained taking into account a structure/function relationship that could also influence anthocyanin intracellular localisation.
authors.affiliation: Dipartimento di Medicina Sperimentale, Sezione di Patologia Generale C Golgi, Università di Pavia, Piazza Botta 10, 27100 Pavia, Italy.
authors.forename: M C
authors.initials: MC
authors.lastname: Lazzé
authors.forename: R
authors.initials: R
authors.lastname: Pizzala
authors.forename: M
authors.initials: M
authors.lastname: Savio
authors.forename: L A
authors.initials: LA
authors.lastname: Stivala
authors.forename: E
authors.initials: E
authors.lastname: Prosperi
authors.forename: L
authors.initials: L
authors.lastname: Bianchi
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019457:Chromosome Breakage; D020552:Comet Assay; D004249:DNA Damage; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism; D016877:Oxidants / Q000633:toxicity; D010084:Oxidation-Reduction; D051381:Rats; D014407:Tumor Cells, Cultured; D020122:tert-Butylhydroperoxide / Q000037:antagonists & inhibitors* / Q000633:toxicity*
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D016877:Oxidants; D020122:tert-Butylhydroperoxide; D005978:Glutathione