Influence of high levels of vitamin E on semen parameters of cocks.
This study was an attempt to find whether the reproductive performance of cocks would be influenced by oral administration of different amounts of dietary vitamin E over a long period of time. For that purpose 60 cocks were divided into five dietary groups of 12 animals each, and supplemented with 0 (control group), 100, 1,000, 10,000 or 20,000 IU alpha-tocopherol/kg diet, respectively, over a period of 12 months. The effect on semen parameters and biochemical parameters measured in pooled semen samples and the weight of the testes were determined. The weight of testes decreased with increasing amounts of supplemented vitamin E. Volume, pH, colour, consistency and motility were not influenced by the diets, but density of ejaculate (sperm/microl), total amount of spermatozoa and morphology of sperm were significantly lowered by increasing amounts of supplemented vitamin E. The alpha-tocopherol concentration in ejaculates increased significantly in relation to the diet whereas phospholipid content and thiobarbituric acid-reactive substances (TBARS) of the semen samples decreased significantly with increasing vitamin E supplementation. The reproductive performance of cocks was negatively influenced by high doses of vitamin E although decreased TBARS indicated rising oxidative defence.
Danikowski
S
S
Institute of Physiological Chemistry, School of Veterinary Medicine, Hannover, Germany.
Sallmann
H-P
HP
Halle
I
I
Flachowsky
G
G
D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D002645:Chickens / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D008297:Male; D023421:Models, Animal; D009929:Organ Size / Q000187:drug effects; D010743:Phospholipids / Q000032:analysis; D011897:Random Allocation; D012661:Semen / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*; D013737:Testis / Q000187:drug effects / Q000502:physiology; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000032:analysis
D000975:Antioxidants; D010743:Phospholipids; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D024502:alpha-Tocopherol