The response of Leuconostoc mesenteroides to low external oxidoreduction potential generated by hydrogen gas.
OBJECTIVE
The physiological consequences of low external oxidoreduction potential in Leuconostoc mesenteroides were investigated.


RESULTS
Leuconostoc mesenteroides was grown under two initial oxidoreduction potential conditions (Eh7: +200 mV and -400 mV) using nitrogen and hydrogen as reducing agents. Growth was affected by Eh7; the lag phase increased from 1 h at an initial Eh7 of +200 mV to 6 h at an initial Eh7 of -400 mV; the maximum specific growth rate at -400 mV was 68% of the one observed at +200 mV. The NADH/NAD+ ratio and (NADH + NAD+) pool were independent of the external Eh7.


CONCLUSIONS
This study shows that changing the external oxidoreduction potential from +200 to -400 mV has a strong effect on the Leuc. mesenteroides physiology. The constancy of the maximum carbon and energetic fluxes (qglu, qATP) under the two Eh7 conditions accompanied by the decrease of YX/S and YATP suggested the existence of an uncoupling phenomenon, namely that some catabolized glucose and hence ATP was not associated with biomass production.


CONCLUSIONS
This paper demonstrates the usefulness of taking into account, the effect of the oxidoreduction potential on the growth of Leuc. mesenteroides in the fermentation process.
Bourel
G
G
Laboratoire de Microbiologie U.M.R. INRA, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation, Université de Bourgogne, 21000 Dijon, France.
Henini
S
S
Diviès
C
C
Garmyn
D
D
D000426:Alcohol Dehydrogenase / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation / Q000502:physiology; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007934:Leuconostoc / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D009243:NAD / Q000378:metabolism; D009584:Nitrogen / Q000502:physiology*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000502:physiology*; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis
D003470:Culture Media; D012329:RNA, Bacterial; D012333:RNA, Messenger; D009243:NAD; D000426:Alcohol Dehydrogenase; D007770:L-Lactate Dehydrogenase; D005947:Glucose; D009584:Nitrogen; D010100:Oxygen