[Sequencing and analysis of flocculation gene(FLO1G)].
The sequence of the flocculation gene (FLO1G) was determined. The result of sequcencing showed that: the cloned gene contains a large open reading frame (ORF) of 3936 bp and encodes for a protein of 1312 amino acid. According to the result of homologous analysis, the cloned gene is homologous to FLO1 but with 675 bp deletion in the ORF region. The missing part belongs to one of the four repeated sequence family of FLO1. Since the cloned DNA fragment can trigger strong flocculence to non-flocculent strain S. cerevisiae YS58, we concluded that the missing part is not the crutical part for the flocculent ability of the gene.
He
Xiuping
X
Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China.
Guo
Wenjie
W
Zhang
Borun
B
Zhang
Pei
P
Lian
Gang
G
Wang
Chenghong
C
Pan
Xueqi
X
D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics*; D005427:Flocculation; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D037241:Mannose-Binding Lectins; D016366:Open Reading Frames; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; C087578:FLO1 protein, S cerevisiae; D005656:Fungal Proteins; D037241:Mannose-Binding Lectins; D029701:Saccharomyces cerevisiae Proteins