title: [Recombinant fowlpox virus expressing HA from subtype H9N2 of avian influenza virus and its protective immunity against homologous challenge in chickens].
abstract: The hemagglutinin (HA) gene from the AIV, A/Chicken/China/F/1998(H9N2), was amplified with the RT-PCR technique and directionally inserted into transferring vector 1175, resulted in recombinant transferring vector 1175HA. In order to generate recombinant fowlpox virus expressing HA(rFPV-HA), the recombinant transferring vector 1175HA was used to transfect the chicken embryo fibroblasts(CEF) pre-infected with wide type fowlpox virus. Then, by selection of blue plaques on the CEF overlaid with agar containing X-gal, rFPV-HA was obtained and purified. The expression of HA by rFPV-HA was detected in the recombinant virus-infected CEF by indirect immunofluorescence. Experiments on chickens demonstrated that rFPV-HA could induce detectable HI antibodies 7 days post-vaccination and those HI antibodies of relatively high titers could persist 55 days. rFPV-HA also had the same protective efficacies to suppress SPF chickens or commercial broiler chickens with antibodies against FPV from shedding challenged virus from intestine as inactivated vaccine in oil emulsion.
authors.affiliation: Key Lab for Animal Infectious Disease, Yangzhou University, Yangzhou 225009, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Cheng
authors.forename: Xiufan
authors.initials: X
authors.lastname: Liu
authors.forename: Daxin
authors.initials: D
authors.lastname: Pen
authors.forename: Hongqi
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D002642:Chick Embryo; D002645:Chickens; D004274:DNA, Recombinant / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism; D005587:Fowlpox virus / Q000235:genetics*; D015870:Gene Expression; D005814:Genes, Viral / Q000235:genetics; D005822:Genetic Vectors; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D053127:Influenza A Virus, H9N2 Subtype; D009980:Influenza A virus / Q000235:genetics*; D011897:Random Allocation; D014162:Transfection; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D004274:DNA, Recombinant; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D014765:Viral Vaccines