[Inhibitory effect of antisense c-myb cDNA on the growth of asynchronous glioma cells in vivo].
OBJECTIVE
To examine the therapeutic effect of oncogene c-myb antisense oligodeoxynucleotide (AON) on rat C6 glioma in vivo.


METHODS
C6 glioma cells were implanted into the subcutis of nude mice, the animals were randomly divided into three groups (12 mice respectively) when the tumor grew about 10 mm in size, and the mice with subcutaneous tumor foci were treated with sense oligodeoxynucleotide (SON), AON and normal saline. Three animals of each group were killed after treatment at 4, 8, 12 and 16 days respectively. The dynamic growth manifestations, features, histopathological changes and apoptosis of the glioma in each group were observed.


RESULTS
AON suppressed the growth of C6 glioma cells, the suppression rates being 66.7%, 71.4%, 72% and 73% at 4, 8, 12, 16 days respectively (P < 0.05). The changes in weight were concurrent with the variations of tumor volume, the tumor weight of AON groups was significantly decreased (P < 0.05). The immunohistochemical assay showed the expression of c-myb proto-oncogenes was significantly decreased in AON groups (P < 0.05). The flow cytometry analysis found the apoptosis cell percentage of AON groups to be 13.4%, 27.1%, 46.1% and 48.4% at 4, 8, 12 and 16 days respectively, and this percentage of AON groups was about 3.5 times the apoptosis cell percentage of SON and control groups at the corresponding time. In addition, noticeable inflammatory infiltration and calcification were observed after the treatment with AON.


CONCLUSIONS
The above findings indicate that c-myb gene might be associated with the suppression of carcinogenesis in brain glioma, and oncogene c-myb might be chosen as a target for antisense gene therapy of gliomas.
Xu
X
X
Department of Neurosurgery, First Affiliated Hospital, WCUMS, Chengdu 6100041, China.
You
C
C
Huang
S
S
Zhang
S
S
Yi
Z
Z
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001932:Brain Neoplasms / Q000473:pathology* / Q000628:therapy; D002455:Cell Division; D005260:Female; D020570:Genes, myb; D015316:Genetic Therapy; D005910:Glioma / Q000473:pathology* / Q000628:therapy; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D011897:Random Allocation
D020319:Oligodeoxyribonucleotides, Antisense