[Influence of EBV latent membrane protein 1 gene on the proliferation of epithelial cells].
In order to study the influence of EBV latent membrane protein (LMP1) gene on the proliferation of epithelial cells and the role of LMP1 creating to epithelial cell carcinomas, EBV LMP1 gene eukaryotic expression plasmid was transfected into human embryo kidney epithelial cells. The expression of LMP1 protein in transfected cells was detected. Observing the formation of colonies in soft agar, the value of MTT absorbed and the expression of PCNA, the results showed that the transfected cells grew vigorously and formed more colonies in soft agar, the MTT uptake and PCNA expression were apparently increased. The conclusion was that LMP1 gene could alter the biological behaviours of epithelial cells, promote cell growth, proliferation and transformation and endowed the transfected cells with growth characteristics like tumor cells.
Chen
Y
Y
Sun Yat-sen Memorial Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510120.
Guo
H
H
Wang
H
H
Li
M
M
D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000196:embryology; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D014162:Transfection; D014763:Viral Matrix Proteins / Q000235:genetics / Q000494:pharmacology*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D018809:Proliferating Cell Nuclear Antigen; D014763:Viral Matrix Proteins