title: [Effects of composition of mobile phases on retention behavior of solutes in hydrophobic interaction chromatography].
abstract: The retention behavior of aromatic alcohol homologues in hydrophobic interaction chromatography (HIC) was investigated for the first time. The differences in retention behavior between proteins and small solutes, and the eluted range and ability of different salts to small solutes and biopolymers, are elucidated with the parameter Z of the stoichiometric displacement retention model. The retention of aromatic alcohols follows the homologue rule. By the comparison of the changes of Z in different salt solutions, it is found that for small molecules the salt type only changes the hydration degree; but for proteins, it not only changes the hydration of proteins and stationary phase, but also affects the conformation of protein molecules near the contact regions between the protein and the stationary phase surface.
authors.affiliation: Shaanxi Province Key Laboratory of Modern Separation Science, Institute of Modern Separation Science, Northwest University, Xi'an 710069, China.
authors.forename: J G
authors.initials: JG
authors.lastname: Zhao
authors.forename: C
authors.initials: C
authors.lastname: Yao
authors.forename: Y M
authors.initials: YM
authors.lastname: Wei
authors.forename: X D
authors.initials: XD
authors.lastname: Geng
mesh_terms: D001704:Biopolymers; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D003574:Cytochrome c Group / Q000032:analysis; D057927:Hydrophobic and Hydrophilic Interactions; D046911:Macromolecular Substances; D009211:Myoglobin / Q000032:analysis* / Q000737:chemistry; D012260:Ribonucleases / Q000032:analysis; D012997:Solvents
chemical_list: D001704:Biopolymers; D003574:Cytochrome c Group; D046911:Macromolecular Substances; D009211:Myoglobin; D012997:Solvents; D012260:Ribonucleases