[Identification and functional analysis of NIFA from Mesorhizobium huakuii 159].
The nifA gene from Mesorhizobium huakuii 159 was identified and sequenced. An open reading frame coding for 44734D. NifA protein would detected. Comparison of the deduced amino acid sequence with other NifA proteins revealed the presence of the conserved central domain and the C-terminal DNA-binding domains. But the predicted M. huakuii NifA protein lacked a N-terminal domain that is present in the homologous proteins. Mutants with Nif- phenotype were obtained by site-directed Tn5 mutagenesis. A nifA constitutively expressed plasmid was constructed, and this plasmid could activate the nifH expression in YMC11.
Gao
C
C
Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai 200032.
Shen
S
S
Jin
R
R
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009586:Nitrogen Fixation / Q000235:genetics*; D016366:Open Reading Frames; D010641:Phenotype; D010957:Plasmids; D012230:Rhizobiaceae / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D001426:Bacterial Proteins; C061338:NifA protein, Bacteria; D014157:Transcription Factors