title: [Research on the immunological cause of auricular pseudocyst].
abstract: OBJECTIVE
  To elucidate the relationship between the auricular pseudocyst and the immunological function of patients.
  
  
  METHODS
  The cyst fluid and blood sample had been detected for contents of IgG, IgA, IgM and complement C3 by radial immunodiffusion, immunocomplex (IC) contents by polyethylene glycol turbidimetry in 55 cases, anti-nuclear antibody (ANA) by immuno-fluorescent technique in 23 cases, extractable nuclear antibody (ENA) by immunotransfer technique in 24 cases. The frozen sections of cyst wall tissue of 24 cases had been detected for immunocomplex by immunoenzyme histochemistric method. The reactions were observed separately between the auricle tissue of healthy white rat, human embryo and cyst fluid, serum of patient and normal serum.
  
  
  RESULTS
  Contents of IgG, IgA, IgM and C3 in the cyst fluid were lower than the serum significantly (P < 0.01). No IC, ANA and ENA could been found both in serum and cyst fluid, but reactive immunocomplex was discovered on the auricle cartilagous tissue of patient, immunocomplex also be observed on human embryo aurical tissue when it reacted with cyst fluid, but complex hadn't be found when the auricle tissue of white rat and human embryo reacted with serum of patient and normal people.
  
  
  CONCLUSIONS
  The reason of auricular pseudocyst may be related with the local autoimmune status of patient.
authors.affiliation: Department of Otorhinolaryngology, First Affiliated Hospital of Guiyang Medical College, Guiyang 550004.
authors.forename: Q
authors.initials: Q
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Fei
authors.forename: T
authors.initials: T
authors.lastname: Zhao
authors.forename: D
authors.initials: D
authors.lastname: Luo
authors.forename: B
authors.initials: B
authors.lastname: Wu
authors.forename: X
authors.initials: X
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015551:Autoimmunity; D003560:Cysts / Q000276:immunology* / Q000473:pathology; D004427:Ear Diseases / Q000276:immunology* / Q000473:pathology; D004431:Ear, External / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged