Independent subsets of amplified fragments from the genome of Neisseria meningitidis identify the same invasive clones of ET37 and ET5.
To determine whether fluorescent amplified-fragment length polymorphism (FAFLP) analysis is an unbiased genome sampling technique, data were analysed from three different primer combinations, amplifying three independent fragment subsets from 123 isolates of Neisseria meningitidis. Using these data, dendrograms were generated with near-identical topologies that identified the same invasive clones of ET37 and ET5 and also identified the same outbreak clusters.
Goulding
Jonathan N
JN
Department of Academic Paediatrics, Imperial College at St Mary's Hospital, London, UK 2Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK 3,4Molecular Bacteriology and Immunology Group, Division of Microbiology3 and Department of Public Health Medicine and Epidemiology4, University of Nottingham, Queen's Medical Centre, Nottingham, UK.
Stanley
John
J
Department of Academic Paediatrics, Imperial College at St Mary's Hospital, London, UK 2Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK 3,4Molecular Bacteriology and Immunology Group, Division of Microbiology3 and Department of Public Health Medicine and Epidemiology4, University of Nottingham, Queen's Medical Centre, Nottingham, UK.
Olver
Will
W
Department of Academic Paediatrics, Imperial College at St Mary's Hospital, London, UK 2Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK 3,4Molecular Bacteriology and Immunology Group, Division of Microbiology3 and Department of Public Health Medicine and Epidemiology4, University of Nottingham, Queen's Medical Centre, Nottingham, UK.
Neal
Keith R
KR
Department of Academic Paediatrics, Imperial College at St Mary's Hospital, London, UK 2Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK 3,4Molecular Bacteriology and Immunology Group, Division of Microbiology3 and Department of Public Health Medicine and Epidemiology4, University of Nottingham, Queen's Medical Centre, Nottingham, UK.
Ala'Aldeen
Dlawer A A
DAA
Department of Academic Paediatrics, Imperial College at St Mary's Hospital, London, UK 2Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK 3,4Molecular Bacteriology and Immunology Group, Division of Microbiology3 and Department of Public Health Medicine and Epidemiology4, University of Nottingham, Queen's Medical Centre, Nottingham, UK.
Arnold
Catherine
C
Department of Academic Paediatrics, Imperial College at St Mary's Hospital, London, UK 2Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK 3,4Molecular Bacteriology and Immunology Group, Division of Microbiology3 and Department of Public Health Medicine and Epidemiology4, University of Nottingham, Queen's Medical Centre, Nottingham, UK.
D015373:Bacterial Typing Techniques / Q000379:methods; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004196:Disease Outbreaks; D005456:Fluorescent Dyes; D005784:Gene Amplification; D016680:Genome, Bacterial; D006801:Humans; D008589:Meningococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D009345:Neisseria meningitidis / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D006113:United Kingdom / Q000453:epidemiology
D004269:DNA, Bacterial; D005456:Fluorescent Dyes