Overexpression of Raidd cDNA inhibits differentiation of mouse preadipocytes.
RAIDD (RIP-associated ICH-1 homologous protein with a death domain) is an adaptor molecule that mediates the action of cysteine proteases involved in apoptosis. To study the possibility of a novel system of cell ablation mediated by RAIDD, a preadipocyte cell line (3T3L1) was stably transfected with a plasmid containing the murine Raidd cDNA under the control of the adipocyte specific promoter aP2. Instead of the expected apoptosis, a blockage to differentiation upon hormonal induction was observed as judged by an absence of lipid accumulation, a lack of expression of adipocyte-specific genes and a fibroblastic appearance. Proliferation rate of Raidd-transfected clones remained unaffected. Overexpression of Raidd cDNA in 3T3L1 cell therefore inhibited differentiation, suggesting that Raidd plays a role in controlling differentiation of mouse preadipocytes and, perhaps, in other cell types, in addition to its established role in apoptosis.
Felmer
R
R
Division of Gene Expression and Development, Roslin Institute, Roslin, Midlothian, Scotland, UK.
Horvat
S
S
Clinton
M
M
Clark
A J
AJ
D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D017209:Apoptosis; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002455:Cell Division; D011416:Complement Factor D; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression; D007700:Kinetics; D051379:Mice; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism
D002352:Carrier Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; D012697:Serine Endopeptidases; D011416:Complement Factor D; C493491:complement factor D, mouse