title: Evaluation of liver fibrosis in chronic hepatitis C with a computer-assisted morphometric method.
abstract: Objective methods are needed to quantitatively assess the burden of fibrous tissue in liver biopsy specimens and its changes after treatment. The aim of this study was to assess the validity of a computer-assisted morphometric method in the evaluation of liver fibrosis in patients with chronic hepatitis C. Sixty-nine liver biopsy specimens stained with Sirius red were evaluated by two independent observers with a computer-assisted morphometric method to measure the percentage of fibrous tissue in the optic fields examined (fibrosis ratio). Furthermore, 11 pairs of liver biopsy specimens obtained before and after treatment from patients with chronic hepatitis C were evaluated with morphometry by two independent observers in order to assess in which direction fibrosis changed. In the 69 patients, the correlation of the morphometry-measured fibrosis ratio pairs by the two observers was high (r = 0.781). However, the differences between paired values were large, reaching +/- 5% in 95% of instances. The fibrosis ratios observed with morphometry by the two examiners correlated poorly with the Ishak's staging score. The two examiners agreed in 10 out of 11 instances in judging in which direction fibrosis had changed. In conclusion, using our present technique of computer-assisted morphometry, the quantitative assessment of the percentage extension of fibrous tissue was not sufficiently accurate. However, computer-assisted morphometry proved to be useful when evaluating the direction of fibrous changes in pairs of liver biopsy specimens from patients with chronic hepatitis C before and after treatment.
authors.affiliation: Clinica Medica Generale, Università degli Studi del Piemonte Orientale Amedeo Avogadro, Ospedale Maggiore della Carità di Novara.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Maduli
authors.forename: Silvano
authors.initials: S
authors.lastname: Andorno
authors.forename: Cristina
authors.initials: C
authors.lastname: Rigamonti
authors.forename: Francesca
authors.initials: F
authors.lastname: Capelli
authors.forename: Stefania
authors.initials: S
authors.lastname: Morelli
authors.forename: Sandra
authors.initials: S
authors.lastname: Colombi
authors.forename: Gabriella
authors.initials: G
authors.lastname: Nicosia
authors.forename: Renzo
authors.initials: R
authors.lastname: Boldorini
authors.forename: Maurizio
authors.initials: M
authors.lastname: Abate
authors.forename: Massimo
authors.initials: M
authors.lastname: Sartori
mesh_terms: D000998:Antiviral Agents / Q000008:administration & dosage; D001706:Biopsy; D019698:Hepatitis C, Chronic / Q000150:complications* / Q000188:drug therapy; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008103:Liver Cirrhosis / Q000473:pathology* / Q000821:virology*; D012720:Severity of Illness Index
chemical_list: D000998:Antiviral Agents