Simple technique of codenaturation and fluorescence in situ hybridization with probes in a microcamera.
We describe a rapid, precise and economical method of performing fluorescence in situ hybridization with probes in a microcamera, without chemical risk for the operator caused by the use of formamide. The application has been tried on metaphase spreads and interphase nuclei from peripheral blood or bone marrow cultures and on formalin-fixed, paraffin-embedded tissue.
Bossi
Bruno
B
Laboratorio Citogenetica, Unità Operativa Anatomia Patologica, Via Michele Coppino 26, 12100, Cuneo, Italy. anapat@scroce.sanitacn.it
Comino
Alberto
A
Dalmasso
Margherita
M
D003582:Cytogenetics / Q000295:instrumentation*; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation / Q000379:methods*; D007399:Interphase; D008677:Metaphase; D008853:Microscopy / Q000295:instrumentation*; D009691:Nucleic Acid Denaturation; D016612:Paraffin Embedding; D012449:Safety; D013048:Specimen Handling; D013696:Temperature; D016707:Tissue Fixation