title: [Analysis of rRNA gene restriction fragments length polymorphism of Leptospira in China].
abstract: Sixty-four Leptospira international and domestic reference strains, which belonging to fifty-four serovars, and twenty-seven field strains were examined by using EcoR I restriction endonuclease analysis of genomic DNA and restriction fragments length polymorphism of rRNA gene, fifty-six Leptospiral ribotypes(RTs) were described. Most serovars gave specific patterns. Serovars in the same serogroup possess common core-segments, but we found RTs of reference strains from China and other countries are different. Most field strains have RTs with correspond of reference strains, only a few bands were shown different if RTs were different. A notable result was that the field strains of serovar pomona have the same Rt as the international reference strain but different from the domestic reference strain.
authors.affiliation: Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206.
authors.forename: X
authors.initials: X
authors.lastname: Feng
authors.forename: J
authors.initials: J
authors.lastname: Qin
authors.forename: Z
authors.initials: Z
authors.lastname: Liang
authors.forename: M
authors.initials: M
authors.lastname: Shi
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Nie
mesh_terms: D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D007919:Leptospira / Q000145:classification* / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics*; D015183:Restriction Mapping; D012703:Serotyping
chemical_list: D004269:DNA, Bacterial; D012335:RNA, Ribosomal