title: Control of completeness and immediate detection of bleeding by a single laser-Doppler flow probe during intravascular middle cerebral artery occlusion in rats.
abstract: To detect the unintentional generation of subarachnoid hemorrhage (SAH) during thread occlusion of the middle cerebral artery (MCA) in rats, a single laser-Doppler flowmetry (LDF) probe was positioned over the ipsilateral cortex. Occlusion of the MCA resulted in an immediate drop of the LDF signal to 21.4+/-8.0% which was followed by a partial recovery to 31+/-11% in animals without SAH. In contrast, a sustained decrease to 7.4+/-5% (P<0.05 vs. non-SAH) occurred in animals with SAH. Thus, unintentional rupturing of the intracerebral carotid artery can easily and reliably be detected by single LDF monitoring immediately after MCA occlusion.
authors.affiliation: Department of Neurosurgery, University Hospital Mannheim, University of Heidelburg, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany. johannes.woitzik@nch.ma.uni-heidelberg.de
authors.forename: Johannes
authors.initials: J
authors.lastname: Woitzik
authors.forename: Lothar
authors.initials: L
authors.lastname: Schilling
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D001921:Brain / Q000098:blood supply / Q000473:pathology; D004195:Disease Models, Animal; D020925:Hypoxia-Ischemia, Brain / Q000209:etiology / Q000473:pathology*; D020244:Infarction, Middle Cerebral Artery / Q000150:complications / Q000473:pathology* / Q000503:physiopathology*; D017078:Laser-Doppler Flowmetry / Q000295:instrumentation* / Q000379:methods*; D008297:Male; D020768:Middle Cerebral Artery / Q000503:physiopathology / Q000601:surgery; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013345:Subarachnoid Hemorrhage / Q000175:diagnosis* / Q000209:etiology / Q000503:physiopathology*