title: Marathon running alters the DNA base excision repair in human skeletal muscle.
abstract: Reactive oxygen and nitrogen species generated either as products of aerobic metabolism or as a consequence of environmental mutagens, oxidatively modify DNA. Formamidopyrimidine-DNA glycosylase (Fpg) and endonuclease III (endo III) or their functional mammalian homologues repair 7,8-dihydro-8-oxoguanine (8-oxoG) and damaged pyrimidines, respectively, to curb the deleterious effects of oxidative DNA alterations. A single bout of physical exercise can induce oxidative DNA damage. However, its effect on the activity of repair enzymes is not known. Here we report that the activity of a functional homolog of Fpg, human 8-oxoG DNA glycosylase (hOGG1), is increased significantly, as measured by the excision of 32P labeled damaged oligonucleotide, in human skeletal muscle after a marathon race. The AP site repair enzyme did not change significantly. Despite the large individual differences among the six subjects measured, data suggest that a single-bout of aerobic exercise increases the activity of hOGG1 which is responsible for the excision of 8-oxoG. The up-regulation of DNA repair enzymes might be an important part of the regular exercise induced adaptation process.
authors.affiliation: Laboratory of Exercise Physiology, School of Sport Sciences, Semmelweis University, Alkotas u. 44, H-1123, Budapest, Hungary. radak@mail.hupe.hi
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Radák
authors.forename: Peter
authors.initials: P
authors.lastname: Apor
authors.forename: Jozsef
authors.initials: J
authors.lastname: Pucsok
authors.forename: Istvan
authors.initials: I
authors.lastname: Berkes
authors.forename: Helga
authors.initials: H
authors.lastname: Ogonovszky
authors.forename: Gabor
authors.initials: G
authors.lastname: Pavlik
authors.forename: Hideko
authors.initials: H
authors.lastname: Nakamoto
authors.forename: Sataro
authors.initials: S
authors.lastname: Goto
mesh_terms: D001706:Biopsy; D004247:DNA / Q000737:chemistry; D004249:DNA Damage; D004260:DNA Repair; D043382:DNA-Formamidopyrimidine Glycosylase; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004706:Endodeoxyribonucleases / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D006801:Humans; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology*; D009699:N-Glycosyl Hydrolases / Q000235:genetics* / Q000378:metabolism; D012420:Running
chemical_list: D029968:Escherichia coli Proteins; D004247:DNA; D004706:Endodeoxyribonucleases; D043224:Deoxyribonuclease (Pyrimidine Dimer); C106403:NTH protein, E coli; D009699:N-Glycosyl Hydrolases; D043382:DNA-Formamidopyrimidine Glycosylase