Mechanisms implicated in the effects of boron on wound healing.
Recently, we demonstrated that boron modulates the turnover of the extracellular matrix and increases TNFalpha release. In the present study, we used an in vitro test to investigate the direct effect of boron on specific enzymes (elastase, trypsin-like enzymes, collagenase and alkaline phosphatase) implicated in extracellular matrix turnover. Boron decreased the elastase and alkaline phosphatase activity, but had no effect on trypsin and collagenase activities. The effect of boron on the enzyme activities was also tested in fibroblasts considered as an in vivo test. In contrast to the results obtained in vitro, boron enhanced the trypsin-like, collagenase, and cathepsin D activities in fibroblasts. Boron did not modify the generation of free radicals compared to the control and did not seem to act on the intracellular alkaline phosphatase activity, However, as it did enhance phosphorylation, it can be hypothesized that boron may affect living cells via a mediator, which could be TNFalpha whose transduction signal involves a cascade of phosphorylations.
Nzietchueng
Rosine Mayap
RM
Laboratory of Medical Biochemistry, School of Medicine, University Henri Poincare Nancy I, Vandoeuvre Lès Nancy, France.
Dousset
Brigitte
B
Franck
Patricia
P
Benderdour
Mohamed
M
Nabet
Pierre
P
Hess
Ketsia
K
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001895:Boron / Q000494:pharmacology*; D002478:Cells, Cultured; D017364:Collagenases / Q000378:metabolism*; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D010196:Pancreatic Elastase / Q000378:metabolism*; D010766:Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism*; D014945:Wound Healing / Q000187:drug effects*
D017382:Reactive Oxygen Species; D000469:Alkaline Phosphatase; D010196:Pancreatic Elastase; D014357:Trypsin; D017364:Collagenases; D001895:Boron