[Acute toxicity and local stimulate test of cucurbitacinBE polylactic acid nano-particles of targeting cervical lymph nodes].
OBJECTIVE
The aim of this experiment was to study acute toxicity and local stimulation of cucurbitacinBE poly-lactic acid nano-particles (CuBE-PLA-NP) for targeting cervical lymph node.


METHODS
Various doses of CuBE-PLA-NP lyophilization injection and cucurbitacinBE (CuBE) were hypodermically or veno-injected into the mice, and then the drug toxicity and side effects were observed; 50% lethal dose (LD50) was counted. CuBE-PLA-NP lyophilization injection and CuBE were also injected into the quadriceps femoralis muscle of rabbits to observe the local stimulate responses of drugs.


RESULTS
The fifty percent lethal doses of subcutaneous and venous CuBE-PLA-NP in mice were 16.74 mg/kg +/- 1.83 mg/kg and 5.60 mg/kg +/- 0.83 mg/kg respectively, that were higher than those of CuBE which were 8.63 mg/kg +/- 1.00 mg/kg and 2.50 mg/kg +/- 0.30 mg/kg respectively. Local stimulate response in rabbit quadriceps femoralis muscle after CuBE-PLA-NP and CuBE injected are 0 grade and 0.1 grade respectively.


CONCLUSIONS
The acute toxicity of CuBE-PLA-NP is lower than that of CuBE. Both CuBE-PLA-NP and CuBE are adequate for local injection, but the local stimulation of CuBE-PLA-NP is smaller than that of CuBE.
Yang
K
K
Department of Stomatology, First Affiliated Hospital, Chongqing Medical University.
Wen
Y
Y
Li
L
L
Wang
C
C
Wang
X
X
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000633:toxicity*; D016503:Drug Delivery Systems; D005260:Female; D007267:Injections / Q000009:adverse effects; D019344:Lactic Acid / Q000008:administration & dosage / Q000633:toxicity*; D007928:Lethal Dose 50; D008198:Lymph Nodes / Q000187:drug effects / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008863:Microspheres; D009062:Mouth Neoplasms / Q000188:drug therapy; D036103:Nanotechnology; D011091:Polyesters; D011108:Polymers / Q000008:administration & dosage / Q000633:toxicity*; D014315:Triterpenes / Q000008:administration & dosage / Q000633:toxicity*
D000970:Antineoplastic Agents; D011091:Polyesters; D011108:Polymers; D014315:Triterpenes; D019344:Lactic Acid; C033616:poly(lactide)