Cloning and expression analysis of rainbow trout Oncorhynchus mykiss interferon regulatory factor 1 and 2 (IRF-1 and IRF-2).
Two rainbow trout (Oncorhynchus mykiss) genes for interferon regulatory factors (IRF) 1 and 2 have been cloned and sequenced. The IRF-1 cDNA contains an open reading frame (ORF) of 996 nucleotides that translates into a 331 amino-acid putative peptide, with a 5' untranslated region (UTR) of 145bp and a 3' UTR of 481bp. The IRF-2 cDNA contains a 1035bp ORF that translates into a 344 amino-acid putative peptide, with a 5' UTR of 146bp and a 3' UTR of 925bp. In vivo, IRF-1 and IRF-2 are constitutively expressed in head kidney, gill and spleen but not liver. Both genes were induced in all the tissues examined. IRF-1 but not IRF-2 expression was significantly increased at the site of injection 1 week after DNA vaccination against viral haemorrhagic septicaemia virus. In vitro, IRF-1 and IRF-2 transcripts are present in unstimulated rainbow trout gonad cells and are up-regulated by poly I/C.
Collet
Bertrand
B
Department of Zoology, Scottish Fish Immunology Research Centre, University of Aberdeen, Tillydrone Avenue, Aberdeen, AB24 2TZ, UK.
Hovens
Guido C J
GC
Mazzoni
David
D
Hirono
Ikuo
I
Aoki
Takashi
T
Secombes
Christopher J
CJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D050856:Interferon Regulatory Factor-1; D050836:Interferon Regulatory Factor-2; D008969:Molecular Sequence Data; D029141:Novirhabdovirus / Q000276:immunology; D017686:Oncorhynchus mykiss / Q000276:immunology*; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics*; D012097:Repressor Proteins; D014157:Transcription Factors; D019444:Vaccines, DNA / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology
D004268:DNA-Binding Proteins; D050856:Interferon Regulatory Factor-1; D050836:Interferon Regulatory Factor-2; D010750:Phosphoproteins; D012097:Repressor Proteins; D014157:Transcription Factors; D019444:Vaccines, DNA; D014765:Viral Vaccines