[Establishment of hybridoma secreting anti-mycobacteria monoclonal antibody by using electrofusion technique].
By using the electrofusion technique, the fusion of NS1 myeloma cells with spleen cells of BALB/c mouse immunized with five species of mycobacteria was carried out. With the optimal electrofusion conditions, we obtained a high fusion efficiency of 1.75 hybrid cell clones per 10(5) spleen cells. ELISA was used for screening secreting antimycobacteria McAb hybrid cells. After cloning, 22 stable hybrid cells lines were selected, and 8 of them had been studied.
Guo
M
M
Department of Medical Imaging, Tianjin Medical University, Tianjin 300203.
Pan
Z
Z
Wang
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002459:Cell Fusion / Q000379:methods*; D002460:Cell Line; D006825:Hybridomas / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000473:pathology; D009161:Mycobacterium / Q000276:immunology*; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal