Simultaneous determination of oleuropein and its metabolites in plasma by high-performance liquid chromatography.
A method based on high-performance liquid chromatography (HPLC) with a diode array detection system was developed and validated aiming at the simultaneous determination of oleuropein (OE) and its metabolites, hydroxytyrosol (HT) and tyrosol (T), in human plasma. These phenolic components are believed to play a vital role in the prevention of coronary artery disease and atherosclerosis. The proposed method includes a clean-up solid-phase extraction procedure (using a C(18) column) with high recovery efficiency (85-100%). The statistical evaluation of the method reveals good linearity, accuracy and reproducibility for all the compounds analyzed with RSD values less than 6.5%, while the detection limit is 50 ng/ml for both OE and T and 75 ng/ml for HT. This assay can be employed in bioavailability studies of olive oil phenolic compounds, thus assisting the evaluation of their pharmacological role.
Tsarbopoulos
Anthony
A
GAIA Research Center, Bioanalytical Department, The Goulandris Natural History Museum, 13 Levidou street, Kifissia, GR-145 62, Greece. atsarbop@gnhm.gr
Gikas
Evangelos
E
Papadopoulos
Nicolaos
N
Aligiannis
Nektarios
N
Kafatos
Anthony
A
D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans; C002769:oleuropein