Rearranged gene order between pig and human in a QTL region on SSC 7.
On porcine Chromosome 7, the region surrounding the MHC region contains QTL influencing many traits including growth, back fat thickness, and carcass composition. Towards the identification of the responsible gene(s), this article describes an increase of density of the radiated hybrid map of SSC 7 in the q11-q14 region and the comparative analysis of gene order on the porcine RH map and human genome assembly. Adding 24 new genes in this region, we were able to build a framework map that fills in gaps on the previous maps. The new software Carthagene was used to build a robust framework in this region. Comparative analysis of human and porcine maps revealed a global conservation of gene order and of distances between genes. A rearranged fragment of around 3.7 Mb was, however, found in the pig approximately 20 Mb upstream from the expected location on the basis of the human map. This rearrangement, found by RH mapping on the IMpRH 7.000 rads panel, has been confirmed by two-color FISH and by mapping on the high resolution IMNpRH2 12.000 rads panel. The rearranged fragment contains two microsatellites found at the most likely QTL location in the INRA QTL experiment. It also contains the BMP5 gene, which, together with CLPS, could be considered as a possible candidate.
Demeure
Olivier
O
Laboratoire de Génétique Cellulaire, INRA, BP 27, 31326 Castanet-Tolosan, France.
Renard
Christine
C
Yerle
Martine
M
Faraut
Thomas
T
Riquet
Juliette
J
Robic
Annie
A
Schiex
Thomas
T
Rink
Anette
A
Milan
Denis
D
D000818:Animals; D002874:Chromosome Mapping; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018895:Microsatellite Repeats; D040641:Quantitative Trait Loci; D013552:Swine / Q000235:genetics*; D026801:Synteny
D005819:Genetic Markers