[The envelope formation of AcMNPV and the location of envelope protein gp64 in host cell].
Electron Microscopic study on the recombinant AcMNPV infected Sf9 cell showed that the virus has at least two forms of envelope formation: one is budding from plasma membrane and the other is enveloping in the nucleus by membrane structure. An additional possible pathway is budding from nuclear membrane. The envelope formation of AcMNPV in Sf9 cell line and its relationship with the envelope protein gp64 were investigated by using immunofluorescence techniques. The results showed gp64 was mainly located on the plasma membrane and nuclear membrane of infected cell. Whereas, there is no gp64 on the envelope of OV virus particles in the nuclei. This pattern cause the differences in envelope composition between the virus particles that budding from plasma membrane and nuclear membrane and those that enveloping in the nucleus.
Wang
X
X
College of Life Sciences, Peking University, Beijing 100871.
Chen
J
J
Gao
W
W
Ding
M
M
Zhai
Z
Z
D000818:Animals; D002460:Cell Line; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000502:physiology*; D011995:Recombination, Genetic; D018411:Spodoptera / Q000166:cytology / Q000821:virology; D014760:Viral Fusion Proteins / Q000378:metabolism*
D014760:Viral Fusion Proteins