Hybridoma cell agglutination as a novel test to detect circulating antigen of Schistosoma japonicum.
We developed a serodiagnostic test which is based on the agglutination of hybridoma cells. In the presence of specific antigen, agglutination of the fixed and stained cells occurs and can be visualized in analogy to traditional erythrocyte agglutination. The procedures were developed with a murine cell line producing a monoclonal antibody against a schistosome gut protein and sera of patients and mice infected with Schistosoma japonicum. This test is capable of detecting circulating antigen during pre-patency in mice infected with 50 cercariae. Its sensitivity was high with acute schistosomiasis japonica (97%, n = 32) and moderate with chronic cases (75%, n = 57). No positive reactions were obtained with healthy persons (n = 78) or patients infected with other parasites (Chlonorchis sinensis, n = 20; Paragonimus westermani, n = 20; Plasmodium vivax, n = 10) or suffering from lupus erythomatodus (n = 5) or mononucleosis (n = 10).
Li
Yong-Long
YL
Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
Liu
Wenqi
W
Ruppel
Andreas
A
D000371:Agglutination; D000372:Agglutination Tests; D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D006801:Humans; D006825:Hybridomas / Q000469:parasitology*; D051379:Mice; D008815:Mice, Inbred Strains; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis*
D000947:Antigens, Helminth