[Expression of alpha-acetolactate decarboxylase gene from Bacillus subtilis in Brewer's yeast].
Yeast YIp-type expression recombinant plasmid(pCMA2-1) was constructed. The expression of alpha-acetolactate decarboxylase gene from Bacillus subtilis was controled by CUP1 promoter and its own terminator. The recombinant plasmid pCMA2-1 was introduced into the brewer's yeast PJ3-5. Transformants were selected using copper resistance as selected marker. The results of activity assay showed that PJ3-5 didn't produce alpha-acetolactate decarboxylase(ALDC), where as the activity of alpha-ALDC in transformants were induced by copper sulfate. The laboratory scale fermentation test confirmed that the total diacetyl concentration was reduced effectively by alpha-ALDC in transformant.
Guo
W
W
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
He
X
X
Tie
C
C
Zhang
B
B
D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D002262:Carboxy-Lyases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D019327:Copper Sulfate / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D003931:Diacetyl / Q000378:metabolism; D005285:Fermentation; D005798:Genes, Bacterial; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D002262:Carboxy-Lyases; C023746:acetolactate decarboxylase; D003931:Diacetyl; D019327:Copper Sulfate