title: On the organic solvent and thermostability of the biocatalytic redox system of Rhodococcus ruber DSM 44541.
abstract: The sec-alcohol dehydrogenase activity of whole cells of Rhodococcus ruber DSM 44541 has been employed as an efficient biocatalytic redox system due to the use of acetone and 2-propanol at elevated concentrations for cofactor regeneration in the oxidation and reduction mode, respectively, and external addition of NADH/NAD(+) can be omitted. The operational half-life time of the redox system is 29 hours in 20% v/v acetone and 37 hours in 30% v/v 2-propanol. The Redox system allows the enantioselective oxidation of sec-alcohols and the asymmetric reduction of ketones to furnish (S)-configurated alcohols in high optical purity. The stability of the cells towards further organic solvents was investigated. In addition, the system displays thermostability of up to 60 degrees C and pH stability of up to pH 11. The system represents a simple to handle tool for environmentally benign redox reactions.
authors.affiliation: Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, A-8010 Graz, Austria.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Stampfer
authors.forename: Birgit
authors.initials: B
authors.lastname: Kosjek
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kroutil
authors.forename: Kurt
authors.initials: K
authors.lastname: Faber
mesh_terms: D019840:2-Propanol / Q000494:pharmacology; D000096:Acetone / Q000494:pharmacology; D000426:Alcohol Dehydrogenase / Q000096:biosynthesis* / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D000438:Alcohols / Q000378:metabolism*; D002384:Catalysis; D004789:Enzyme Activation; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007659:Ketones / Q000378:metabolism*; D009930:Organic Chemicals / Q000494:pharmacology; D010084:Oxidation-Reduction; D012240:Rhodococcus / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D012680:Sensitivity and Specificity; D012997:Solvents / Q000494:pharmacology*; D013045:Species Specificity; D013237:Stereoisomerism; D013696:Temperature
chemical_list: D000438:Alcohols; D007659:Ketones; D009930:Organic Chemicals; D012997:Solvents; D000096:Acetone; D000426:Alcohol Dehydrogenase; D019840:2-Propanol