[Chiral separation of enantiomers of synthesized amlodipine and its intermediate by capillary electrophoresis].
A capillary electrophoretic method for the chiral separation of enantiomers of synthesized amlodipine and its intermediate was developed. Several cyclodextrins (CDs) were applied as the chiral selectors and it was found that the ionic modified carboxymethyl-beta-cyclodextrin (CM-beta-CD) could give satisfactory enantioselectivity. In addition, the effects of the pH value of the buffer system, the concentration of the CD and the voltage on the chiral separation were investigated. The optimized buffer for amlodipine and its intermediate enantiomers was a buffer containing 30 mmol/L phosphate and 50 mmol/L CM-beta-CD (pH 6.12). Under these conditions, the resolutions of enantiomers of amlodipine and its intermediate were 1.73 and 1.55, respectively.
Li
Bao-hui
BH
College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China.
Yang
Geng-liang
GL
Wang
De-xian
DX
Zhang
Zhe-feng
ZF
Chen
Yi
Y
D017311:Amlodipine / Q000737:chemistry / Q000302:isolation & purification*; D002021:Buffers; D002121:Calcium Channel Blockers / Q000737:chemistry / Q000302:isolation & purification; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D002021:Buffers; D002121:Calcium Channel Blockers; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C441457:carboxymethyl-beta-cyclodextrin; D017311:Amlodipine