[The establishment of two new evaluation criteria for cell state of plant cell suspension cultures].
An experiment on suspending rice cell cultures in sucrose solution and on optical density (OD) value in culture solution at 576 nm wave width was carried out, the result indicates that suspension rate (%) of suspensions, while suspending in 0.76 mol/L sucrose solution for 15 minutes at room temperature, can be employed as an judgment criterion of cell state. Suspending cell suspension in 0.76 mol/L sucrose solution is also a good approach on selecting cells from suspension cell mass, as a result, it is beneficial to establishing cell suspension. The browning of cell suspension is related to the necrosis degree of suspension cells. OD value of cell suspension liquid at 576 nm can be taken as a criterion with which the necrosis degree of cell suspension can be evaluated.
Chen
B T
BT
National Key Laboratory of Crop Genetic Improvements, Huazhong Agricultural University, Wuhan 430070.
Xie
Y F
YF
Zhang
D P
DP
Yu
Y J
YJ
D018929:Cell Culture Techniques / Q000592:standards*; D003470:Culture Media; D012275:Oryza / Q000166:cytology*
D003470:Culture Media