Organotypic slice cultures from transgenic mice as disease model systems.
Organotypic slice cultures have been prepared from the brains of transgenic mice with Alzheimer's disease-type pathology. Cell types within the slice undergo differentiation and slices can be maintained in culture for up to 6 mo when prepared from young neonates. Slices have been prepared from mice overexpressing genes of relevance to Alzheimer's disease, including mutant or wild-type tau. Neurons in these slices develop neurons that are immunoreactive for a number of markers of abnormal tau. Organotypic slice models are currently being used to test the impact of tangle enhancers or inhibitors as a prescreen for efficacy before testing drugs in vivo.
Duff
Karen
K
Nathan Kline Institute and Department of Psychiatry, New York University, 140 Old Orangeburg Rd, Orangeburg, NY 10962, USA. duff@nki.rfmh.org
Noble
Wendy
W
Gaynor
Kayte
K
Matsuoka
Yasuji
Y
D000544:Alzheimer Disease / Q000473:pathology*; D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation; D046508:Culture Techniques / Q000379:methods*; D004195:Disease Models, Animal; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D010766:Phosphorylation; D016875:tau Proteins / Q000096:biosynthesis / Q000235:genetics
D015415:Biomarkers; D016875:tau Proteins