[Determination of activity of bone alkaline phosphatase in whole serum by high performance liquid chromatography].
An analytical method has been established for the determination of bone alkaline phosphatase (BALP) activity using high performance liquid chromatography(HPLC). The stainless steel column was 22 cm x 4.6 mm i.d. packed with totally porous, spherical silica particles. A solution of methanol and 0.01 mol/L CH3COOH(30/70, V/V) adjusted to pH 4.8 was employed as the mobile phase. The flow rate was 0.8 mL/min. Chromatography was performed with ultraviolet detector at 275 nm. Linear calibration curve for BALP was measured within the range of 8-200 mg/L with correlation coefficient of 0.996. The lowest detection limit was 3 ng. The HPLC method described is simple, rapid and reliable and suitable for clinical monitoring.
Zhang
Y M
YM
Jinzhou Medical College, Jinzhou 121001, China. zhangym6311@263.net
Bai
X Z
XZ
Chen
Z W
ZW
Zhang
C X
CX
D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D001842:Bone and Bones / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007527:Isoenzymes / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley
D007527:Isoenzymes; D000469:Alkaline Phosphatase