[Determination of O6-methylguanine by high performance capillary zone electrophoresis].
In this paper, a method to determine O6-methylguanine in urine by high performance capillary zone electrophoresis has been established. O6-methylguanine was directly introduced into the capillary employing sodium borate at pH 9.0 as buffer. The capillary used was 75 microns i.d. x 57 cm. The calibration curve showed good linearity, r = 0.9987, CVs were 1.51% for intra-day and 1.93% for inter-day. The average recovery was 98.2%. In conclusion, this method is a simple, rapid, precise and reliable technique for O6-methylguanine measurement in urine.
Du
W
W
Medical College of Qingdao University, Qingdao 266021, China.
Liang
H
H
Zhang
X Z
XZ
D018736:DNA Adducts; D019075:Electrophoresis, Capillary / Q000379:methods*; D006147:Guanine / Q000031:analogs & derivatives* / Q000652:urine*; D006801:Humans; D009153:Mutagens / Q000032:analysis*
D018736:DNA Adducts; D009153:Mutagens; D006147:Guanine; C008449:O-(6)-methylguanine