title: [Cloning, sequencing of beagle E-selectin genomic DNA and comparison of it with other species].
abstract: OBJECTIVE
  Selectins are a group of members of Cell Adhesion Molecular family; they play very important roles in inflammation and neovascularization. The aim of this study is to clone, sequence Beagle E-selectin genomic DNA and make homogeneous analyses to compare it with those of other animals.
  
  
  METHODS
  Twelve fragments of genomic DNA of Beagle E-selectin were obtained by PCR, and the 12 fragments were cloned in T-vector. At last, the genomic DNA sequences of Beagle E-selectin were obtained through gene sequencing, assembling and base calling, and the gene structure was analyzed.
  
  
  RESULTS
  The sequence of Beagle E-selectin genomic DNA that contained the whole open reading box was acgured. The results of homogeneous analyses showed a great extent of E-selectin gene homology among human, porcine and dog.
  
  
  CONCLUSIONS
  The demonstration of a large extent of the gene homology has substantiated in this connection the important roles of E-selectin in transplantation immunity and neoplasm development.
authors.affiliation: Department of Cardiovascular & Thoracic Surgery, First Affiliated Hospital, WCUMS, Chengdu 610041, China.
authors.forename: L
authors.initials: L
authors.lastname: Zheng
authors.forename: Y
authors.initials: Y
authors.lastname: Shi
authors.forename: L
authors.initials: L
authors.lastname: Dong
authors.forename: G
authors.initials: G
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Cheng
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004285:Dogs; D019040:E-Selectin / Q000235:genetics* / Q000502:physiology; D016678:Genome; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine
chemical_list: D019040:E-Selectin