[Determination of degraded products of penicillin by high performance liquid chromatography].
A method for the determination of main composition in degraded products of penicillin, 6-aminopenicillanic acid (6-APA), penicillin G kalium (PGK), phenylacetic acid (PAA), and the by-product benzylpenicilloic acid (BPA) by HPLC was studied. Separation conditions were as follows: Spherisorb C18 column, 250 mm x 4.6 mm i.d., 10 micrograms; mobile phase: V (methanol): V(0.004 mol/L KH2PO4 buffer, pH 4.5) = 50:50; flow rate: 1 mL/min; detector: UV 230 nm. The method is effective, quick, accurate and reproducible. The satisfactory results show that this new method has certain practical values as an approach of quality control.
Zhao
J M
JM
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. zhaomei@dicp.ac.cn
Du
J Y
JY
Zhang
M J
MJ
D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D010397:Penicillanic Acid / Q000031:analogs & derivatives* / Q000032:analysis*; D010399:Penicillin Amidase / Q000032:analysis; D010400:Penicillin G / Q000032:analysis* / Q000737:chemistry; D010648:Phenylacetates / Q000032:analysis*
D010648:Phenylacetates; D010397:Penicillanic Acid; D010399:Penicillin Amidase; C025136:phenylacetic acid; D010400:Penicillin G; C004408:aminopenicillanic acid