[Molecular cloning of full length capsanthin/capsorubin synthase homologous gene from orange (Citrus sinensis)].
The complete sequence of orange homologous capsanthin/capsorubin synthase gene is 3788 bp long with a coding sequence of 1512 bp, which encodes a polypeptide of 503 amino acids. The 5' upstream sequence is 1721 bp long and the 3' downstream sequence is 555 bp long. The amino acid sequence of this gene is 78% and 69% identical to the genes from carrot and pepper, respectively. It is also partially homologous to plant neoxanthin synthase, lycopene beta-cyclase and lycopene epsilon cyclase genes. Isolation of the gene provides a framework for elucidation of the mechanisms involved in inability of citrus to produce capsanthin and capsorubin.
Xu
C J
CJ
Department of Horticulture, Huajiachi Campus, Zhejiang University, Hangzhou 310029.
Chen
D M
DM
Zhang
S L
SL
D000595:Amino Acid Sequence; D032084:Citrus sinensis / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D010940:Plant Proteins; D010088:Oxidoreductases; C086535:CCS protein, Capsicum annuum