[Analysis of a novel platinum anticancer compound HJ5 by high performance liquid chromatography].
Cis-dichloroamine (4-amino-2, 2, 6, 6-tetramethylpiperidine-1-oxyl) platinum (II) (abbreviated to HJ5) is a novel platinum anticancer compound which has prospects for research and application. In order to establish a conventional analytical method, an HPLC has been studied. Under the conditions of acetonitrile-water (10:90, volume ratio) as mobile phase, detection wavelength of 210 nm, a Phenomenex column C18(150 mm x 3.9 mm i.d., 10 microns). The linear equation, recovery and accuracy of the method were determined with external standard method. The HPLC has satisfactory resolution between the peaks of HJ5 and impurities. The peak areas of HJ5 were linear to its amounts detected and the detection limit was 0.07 microgram. The hydration of HJ5 can be inhibited effectively by NaCl solution. In a 9 g/L NaCl solution, the stability can be kept for more than 2 hours which is enough for analysis.
Liu
Yang
Y
Research Center of Precious Metal-Based Drugs, Kunming Institute of Precious Metals, Kunming 650221, China.
He
Jian
J
Liu
Zhu-dong
ZD
Chen
Xi-zhu
XZ
Liu
Wei-ping
WP
D000970:Antineoplastic Agents / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002945:Cisplatin / Q000032:analysis / Q000737:chemistry; D003497:Cyclic N-Oxides / Q000032:analysis* / Q000737:chemistry; D009944:Organoplatinum Compounds / Q000032:analysis* / Q000737:chemistry
D000970:Antineoplastic Agents; D003497:Cyclic N-Oxides; D009944:Organoplatinum Compounds; C477049:dichloroamine(4-amino-2,2,6,6-tetramethylpiperidin-1-oxyl)platinum(II); D002945:Cisplatin