Fluoroquinolone resistance in Streptococcus pyogenes.
Streptococcus pyogenes isolated from blood and urine samples obtained from a 78-year-old woman was tested for susceptibility, and fluoroquinolone resistance (minimum inhibitory concentration of levofloxacin, 16 microg/mL) was found. DNA amplification and sequencing revealed a serine81-->tyrosine substitution in gyrA and 2 substitutions in parC: serine79-->phenylalanine and alanine121-->valine. This is the second report of a clinical isolate of S. pyogenes with high-level fluoroquinolone resistance.
Richter
Sandra S
SS
Department of Pathology, University of Iowa Healthcare, Iowa City, Iowa 52242, USA. sandra-richter@uiowa.edu
Diekema
Daniel J
DJ
Heilmann
Kris P
KP
Almer
Laurel S
LS
Shortridge
Virginia D
VD
Zeitler
Rod
R
Flamm
Robert K
RK
Doern
Gary V
GV
D019943:Amino Acid Substitution; D000890:Anti-Infective Agents / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005260:Female; D006801:Humans; D064704:Levofloxacin; D008826:Microbial Sensitivity Tests; D015242:Ofloxacin / Q000494:pharmacology; D013290:Streptococcal Infections / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
D000890:Anti-Infective Agents; D064704:Levofloxacin; D015242:Ofloxacin; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase