Optimization and validation of conventional and micellar LC methods for the analysis of methyltestosterone in sugar-coated pills.
Two isocratic liquid chromatographic methods (conventional and micellar) for the determination of methyltestosterone in sugar-coated pills using fluoxymesterone as internal standard have been developed and validated. In conventional liquid chromatography a mobile phase 45% water:acetonitrile 55% (v:v), a flow-rate 1 mlmin(-1) and a C(18) Hypersil ODS (250 x 4.6 mm, 5 microm) column (25 degrees C) were used. In micellar liquid chromatography the conditions were: mobile phase 40 mM sodium dodecyl sulfate: 10% propanol, flow-rate 0.5 mlmin(-1) and C(18) Hypersil ODS (150 x 3.0 mm, 5 microm) column (60 degrees C). For both methods, UV absorbance detection at 245 nm was used and a separation up to base line was achieved. Prior to HPLC analysis a simple sample preparation was required.
Gonzalo-Lumbreras
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Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense, 28040 Madrid, Spain.
Izquierdo-Hornillos
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D045930:Anabolic Agents / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005474:Fluoxymesterone / Q000032:analysis; D007202:Indicators and Reagents; D008777:Methyltestosterone / Q000032:analysis*; D008823:Micelles; D012015:Reference Standards; D015203:Reproducibility of Results; D013607:Tablets
D045930:Anabolic Agents; D007202:Indicators and Reagents; D008823:Micelles; D013607:Tablets; D005474:Fluoxymesterone; D008777:Methyltestosterone