A novel application of thermo-responsive polymer to affinity precipitation of polysaccharide.
The lectin receptors concanavalin A (ConA) and wheat germ lectin (WGL) were successfully conjugated to a thermo-responsive hydrogel polymer (poly-N-isopropylacrylamide) (PNIPAAm) as an affinity ligand. The coupling efficiencies of the ConA and WGL to PNIPAAm were 5.1% and 44%, respectively. These lectin receptor-polymer conjugates were then tested for their efficiency in purification of various polysaccharides or polysaccharide-containing compounds such as beta-glucan. Results indicated that these conjugates separated various polysaccharides from a complex mixture. The use of a thermo-responsive polymer in low-temperature purification of potentially heat-labile glycoproteins is advantageous. Additionally, other affinity ligands could be coupled to the polymer for separation of the respective bioactive molecules.
Pan
Li-Chun
LC
Biomedical Engineering Center, Industrial Technology Research Institute, Chutung, Hsinchu 310, Taiwan, ROC.
Chien
Chih-Ching
CC
D000180:Acrylic Resins / Q000737:chemistry; D003208:Concanavalin A / Q000737:chemistry; D005936:Glucans / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D037102:Lectins / Q000737:chemistry; D008024:Ligands; D009844:Oligosaccharides; D011108:Polymers / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D011485:Protein Binding; D013696:Temperature; D013997:Time Factors; D014909:Wheat Germ Agglutinins / Q000737:chemistry
D000180:Acrylic Resins; D005936:Glucans; D037102:Lectins; D008024:Ligands; D009844:Oligosaccharides; D011108:Polymers; D011134:Polysaccharides; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; C052970:poly-N-isopropylacrylamide; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate