[Effect of lipopolysaccharide on recombinant human osteogenic protein-1-induced responses in human dental pulp cells].
OBJECTIVE
To investigate the effects of lipopolysaccharide (LPS) on recombinant human osteogenic protein-1 (rhOP-1) induced proliferation and differentiation.


METHODS
Measured MTT, alkaline phosphatase (ALPase) activity and osteocalcin content of human dental pulp cells.


RESULTS
Low-dose LPS (1 microgram/ml) caused co-operative effect with low concentration of rhOP-1 to enhance human dental pulp cell proliferation and ALPase activity, but not to osteocalcin, whereas, high-dose LPS (100 micrograms/ml) significantly inhibited the proliferation, ALPase activity and osteocalcin with or without rhOP-1.


CONCLUSIONS
The role of LPS in caries-induced reparative dentin might be a significant determinant.
Su
Q
Q
College of Stomatology, West China University of Medical Sciences.
He
G
G
Zhang
Y
Y
Li
Q
Q
D000469:Alkaline Phosphatase / Q000187:drug effects; D055395:Bone Morphogenetic Protein 1; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D004357:Drug Synergism; D016967:Fusobacterium nucleatum / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008666:Metalloendopeptidases / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology
D019485:Bone Morphogenetic Proteins; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D000469:Alkaline Phosphatase; D008666:Metalloendopeptidases; C525705:BMP1 protein, human; D055395:Bone Morphogenetic Protein 1