Usefulness of K-1 (CD-30) marker in Hodgkin's disease.
OBJECTIVE
To identify Hodgkin's and Reed Sternberg like cells by a single Immunomarker (Ki-1; CD-30) and to highlight diagnostic specificity of these cells in Hodgkin's disease, as these cells are frequently encountered in other lymphoproliferative disorders on H&E sections.


METHODS
Seventy-nine histologically diagnosed cases of lymph node biopsies (59 cases of Hodgkin's disease, 15 cases of Non Hodgkin's lymphoma and 5 cases of non neoplastic lymphoid tissue) were subjected to Ki-1 (CD-30) immunostaining as all cases revealed Hodgkin's and/or Reed Sternberg like cells on H&E stained sections.


RESULTS
Out of 59 cases of Hodgkin's disease, 49 (83%) showed Ki-1 (CD-30) immunoreactivity for Reed Sternberg and Hodgkin's cells. None of the 15 cases of non-Hodgkin's lymphoma (2 small lymphocyte type, 11 diffuse large cells type, 2 lymphoblastic type) showed positive Ki-1 (CD-30) immunostaining. Only one case of the remaining 5 cases of non-neoplastic lymphoid lesion (2 toxoplasmic lymphadenitis, one histiocytic necrotizing lymphadenitis, one reactive hyperplasia and one chronic non specific lymphadenitis) showed positive Ki-1 immunostaining.


CONCLUSIONS
Ki-1 (CD-30) is a reliable marker for Hodgkin's Reed Sternberg like cells in histologically proven cases of Hodgkin's disease but not for similar cells encountered in other lymhoproliferative lesions.
Shakoor
K A
KA
Department of Pathology, BMSI, Jinnah Postgraduate Medical Centre, Karachi.
Saleh
A
A
Khanzada
M S
MS
D014408:Biomarkers, Tumor / Q000378:metabolism*; D006689:Hodgkin Disease / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D017730:Ki-1 Antigen / Q000378:metabolism*; D008198:Lymph Nodes / Q000378:metabolism*; D016539:Reed-Sternberg Cells / Q000276:immunology*
D014408:Biomarkers, Tumor; D017730:Ki-1 Antigen