Recombinant congenic strains of mice: a new tool for the genetic dissection of efficacy and toxicity of adjuvants.
The efficacy of immunization of a vaccine depends on an antigen, an adjuvant, and on the expression of multiple genes in the host. The responsiveness of various strains of mice to adjuvants is therefore dependent on their genetic background present as a complex, multigenic trait similarly as in man. We have recently developed a gene-discovery platform, termed recombinant congenic strains (RCS), that greatly facilitates the dissection, localization and characterization of genes that mediate complex traits such as responsiveness to adjuvants. These recombinant congenic mice, which were constructed from two progenitor strains (A/J and C57BL/6) that are phenotypically different for several spontaneous or infectious diseases, were generated such that they carry 13.5% of the one genome in 85% of the other genome. The use of these RCS mice therefore enables a more efficient identification of genes that mediate the responsiveness of the adjuvant.
Di Marco
Sergio
S
The Research Institute of the McGill University Health Center, 1650 Cedar Avenue L11-218, Montreal, Que, Canada H3G 1A4.
Moisan
Jacques
J
Boghdady
Marie-Linda
ML
Skamene
Emil
E
Radzioch
Danuta
D
D000276:Adjuvants, Immunologic / Q000494:pharmacology* / Q000633:toxicity*; D000818:Animals; D004351:Drug Resistance / Q000235:genetics / Q000276:immunology; D005260:Female; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D020297:Mice, Congenic; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D010641:Phenotype; D011956:Receptors, Cell Surface / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism; D011995:Recombination, Genetic; D051193:Toll-Like Receptors
D000276:Adjuvants, Immunologic; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D051193:Toll-Like Receptors