title: Solution structure of a tmRNA-binding protein, SmpB, from Thermus thermophilus.
abstract: Small protein B (SmpB) is required for trans-translation, binding specifically to tmRNA. We show here the solution structure of SmpB from an extremely thermophilic bacterium, Thermus thermophilus HB8, determined by heteronuclear nuclear magnetic resonance methods. The core of the protein consists of an antiparallel beta-barrel twisted up from eight beta-strands, each end of which is capped with the second or third helix, and the first helix is located beside the barrel. Its C-terminal sequence (20 residues), which is rich in basic residues, shows a poorly structured form, as often seen in isolated ribosomal proteins. The results are discussed in relation to the oligonucleotide binding fold.
authors.affiliation: Department of Industrial Chemistry, Faculty of Engineering, Chiba Institute of Technology, Chiba 275-0016, Japan.
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Someya
authors.forename: Nobukazu
authors.initials: N
authors.lastname: Nameki
authors.forename: Haruko
authors.initials: H
authors.lastname: Hosoi
authors.forename: Sakura
authors.initials: S
authors.lastname: Suzuki
authors.forename: Hideki
authors.initials: H
authors.lastname: Hatanaka
authors.forename: Michiko
authors.initials: M
authors.lastname: Fujii
authors.forename: Takaho
authors.initials: T
authors.lastname: Terada
authors.forename: Mikako
authors.initials: M
authors.lastname: Shirouzu
authors.forename: Yorinao
authors.initials: Y
authors.lastname: Inoue
authors.forename: Takehiko
authors.initials: T
authors.lastname: Shibata
authors.forename: Seiki
authors.initials: S
authors.lastname: Kuramitsu
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Gota
authors.initials: G
authors.lastname: Kawai
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D016601:RNA-Binding Proteins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D012996:Solutions; D016963:Thermus thermophilus
chemical_list: D001426:Bacterial Proteins; D016601:RNA-Binding Proteins; D012996:Solutions; C430328:small protein B