Expression of carbonic anhydrase-related protein CA-RP VIII in non-small cell lung cancer.
Carbonic anhydrase-related protein (CA-RP VIII) lacks a Zn-binding motif which is essential for carbonic anhydrase activity. Therefore, CA-RP VIII is believed to have a no catalytic activity and a new biological property. In the present study, CA-RP VIII expression in non-tumorous lung and non-small cell lung carcinomas was investigated. Little or no expression of CA-RP VIII was observed in human lungs by Northern-blot analysis. Reverse-transcription polymerase chain reaction analysis demonstrated CA-RP VIII mRNA in developing human lungs and, to a lesser extent, in normal lungs. Subsequent immunohistochemical staining revealed that CA-RP VIII was expressed in the pulmonary epithelium in developing lungs; however, CA-RP VIII expression was restricted in bronchial ciliated cells in adult lungs. Neither bronchial gland nor squamous metaplasia of interstitial pneumonia expressed CA-RP VIII. In contrast, CA-RP VIII was strongly expressed in almost all archival lung cancer specimens, which included 24 squamous cell carcinomas, 25 adenocarcinomas, and 6 adenosquamous cell carcinomas. Cancer cells at the front of tumor progression expressed CA-RP VIII in particular abundance. The present findings suggest that CA-RP VIII may play a role in non-small lung cell carcinomas.
Akisawa
Yousuke
Y
First Department of Internal Medicine, Kochi Medical School, Nankoku, Kochi 783-8505, Japan.
Nishimori
Isao
I
Taniuchi
Keisuke
K
Okamoto
Nobuto
N
Takeuchi
Tamotsu
T
Sonobe
Hiroshi
H
Ohtsuki
Yuji
Y
Onishi
Saburo
S
D000818:Animals; D014408:Biomarkers, Tumor; D015152:Blotting, Northern; D001980:Bronchi / Q000201:enzymology / Q000473:pathology; D002256:Carbonic Anhydrases; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology* / Q000473:pathology; D002452:Cell Count; D017931:DNA Primers / Q000737:chemistry; D004847:Epithelial Cells / Q000201:enzymology / Q000473:pathology; D006801:Humans; D008168:Lung / Q000196:embryology / Q000201:enzymology / Q000473:pathology; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012334:RNA, Neoplasm / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D047908:Intracellular Signaling Peptides and Proteins
D014408:Biomarkers, Tumor; D002256:Carbonic Anhydrases; D017931:DNA Primers; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C512821:Car8 protein, mouse; C512818:CA8 protein, human; D047908:Intracellular Signaling Peptides and Proteins