Genetic diversity of avian infectious bronchitis virus California variants isolated between 1988 and 2001 based on the S1 subunit of the spike glycoprotein.
Twenty-nine isolates of avian infectious bronchitis virus (IBV) recovered from commercial chicken flocks in California between 1988 and 2001 and identified as California variants by serotype and direct automated cycle sequencing of the IBV spike glycoprotein S1 subunit, were further characterized phylogenetically and by nucleotide sequence comparison. California variants were grouped according to production type of chicken, by comparison with public access sequence databases (NCBI GenBank and EMBL), or based on phylogenetic analysis. Fisher's Exact test was used to compare mutations per year, purifying and positive selection, predictive antigenicity, and a > or = 6 bp deletion between California variant groups.A high number of mutations at the nucleotide level ( p = 0.013) and a > or = 6 bp deletion in the nucleotide sequence ( p = 0.006) was significantly associated with broiler-type chickens. However, 88% of significant comparisons at the amino acid level such as purifying and positive selection were seen in layer-type chickens. A pronounced predictive antigenicity in the HVR2 region was also associated with layer-type chickens ( p = 0.001). The study indicates that IBV in California is in a phase of slow evolution with different evolutionary patterns being associated with the production type of chicken.
Schikora
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BM
California Animal Health and Food Safety Laboratory, University of California, Davis 95617, USA. skhietala@ucdavis.edu
Shih
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Hietala
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002140:California / Q000453:epidemiology; D003062:Codon; D022801:Complementarity Determining Regions; D017124:Conserved Sequence; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D014644:Genetic Variation; D006389:Hemagglutinins, Viral / Q000032:analysis; D001351:Infectious bronchitis virus / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D011201:Poultry Diseases / Q000235:genetics / Q000821:virology*; D017386:Sequence Homology, Amino Acid; D012703:Serotyping; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
D003062:Codon; D022801:Complementarity Determining Regions; D006389:Hemagglutinins, Viral; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins