[Analysis of 2-acrylamido-2-methyl propane sulfonic acid by thin layer chromatography].
A method for the analysis of 2-acrylamido-2-methyl propane sulfonic acid(AMPS) by TLC is described. The plate was Silica GF254. We selected benzene/methanol as developing agent from several developing systems and fixed the best ratio: V(benzene):V(methanol) = 2:1. The experiment indicated that the Rf value of AMPS was 0.36 while that of AM(acrylamide), the main impurity in crude AMPS sample, was 0.64. The minimum detectable limit of AMPS was 5 micrograms and the developing time was about 25 minutes. Compared with ether/methanol and chloroform/methanol, benzene/methanol system has the advantages of more easily controlling its ratio, better repeatability and much rapid developing rate. This method may be helpful in the control of AMPS in quality.
Ma
Z
Z
Department of Chemistry, Henan Normal University, Xinxiang 453002, China.
Wei
T
T
Feng
G
G
Liu
J
J
Zhao
Z
Z
D000178:Acrylamides / Q000032:analysis*; D000476:Alkanesulfonates / Q000032:analysis*; D001554:Benzene; D002855:Chromatography, Thin Layer / Q000379:methods; D000432:Methanol
D000178:Acrylamides; D000476:Alkanesulfonates; C056288:2-acrylamido-2-methylpropanesulfonate; D001554:Benzene; D000432:Methanol