title: Budding yeast mms4 is epistatic with rad52 and the function of Mms4 can be replaced by a bacterial Holliday junction resolvase.
abstract: MMS4 of Saccharomyces cerevisiae was originally identified as the gene responsible for one of the collection of methyl methanesulfonate (MMS)-sensitive mutants, mms4. Recently it was identified as a synthetic lethal gene with an SGS1 mutation. Epistatic analyses revealed that MMS4 is involved in a pathway leading to homologous recombination requiring Rad52 or in the recombination itself, in which SGS1 is also involved. MMS sensitivity of mms4 but not sgs1, was suppressed by introducing a bacterial Holliday junction (HJ) resolvase, RusA. The frequencies of spontaneously occurring unequal sister chromatid recombination (SCR) and loss of marker in the rDNA in haploid mms4 cells and interchromosomal recombination between heteroalleles in diploid mms4 cells were essentially the same as those of wild-type cells. Although UV- and MMS-induced interchromosomal recombination was defective in sgs1 diploid cells, hyper-induction of interchromosomal recombination was observed in diploid mms4 cells, indicating that the function of Mms4 is dispensable for this type of recombination.
authors.affiliation: Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Nao
authors.initials: N
authors.lastname: Odagiri
authors.forename: Masayuki
authors.initials: M
authors.lastname: Seki
authors.forename: Fumitoshi
authors.initials: F
authors.lastname: Onoda
authors.forename: Akari
authors.initials: A
authors.lastname: Yoshimura
authors.forename: Sei
authors.initials: S
authors.lastname: Watanabe
authors.forename: Takemi
authors.initials: T
authors.lastname: Enomoto
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D004843:Epistasis, Genetic; D045585:Flap Endonucleases; D045565:Holliday Junction Resolvases; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology; D051721:Rad52 DNA Repair and Recombination Protein; D011995:Recombination, Genetic / Q000187:drug effects / Q000528:radiation effects; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D009153:Mutagens; C497197:RAD52 protein, S cerevisiae; D051721:Rad52 DNA Repair and Recombination Protein; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D008741:Methyl Methanesulfonate; D004706:Endodeoxyribonucleases; D045585:Flap Endonucleases; C473486:CCE1 protein, S cerevisiae; D045565:Holliday Junction Resolvases; C112337:MMS4 protein, S cerevisiae