[Phenotypic features and genetic diversity of strains of Rhodopseudomonas].
14 strains of purple non-sulfur bacteria and 3 type strains of Rhodopseudomonas were investigated by phenotypic characterizing, DNA-DNA hybridizing and DGGE analyzing. The characteristics of their morphology, structure and physiology show that 14 strains of isolates have the features of the genus Rhodopseudomonas: dividing asymmetrically by budding, lamellar intracytoplasmic membranes, presence of bacteriochlorophyll a and carotenoids of the normal spirilloxanthin series. On the base of their differences in morphology, growth, carbon sources 14 strains can be divided into 2 groups: g-c group, T group. Parts of 16S rRNA gene were amplified by using a pair of primers: 341f-906r. Denaturing gradient gel electrophoresis(DGGE) of PCR-amplified fragment of rDNA indicated that they have 3 genetic types: 1 of them, including 6 of 8 strains of T groups is similar to that of Rps. rutila R1, the other 2 types, represented separately by 2 of T group or all the g-c group, were different from that of either of 3 type strains The Data of the DNA-DNA hybridization of 8 representative strains indicated that the differences between different types were enough to separate them into different species including 2 possible new species.
Zhang
D
D
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
Huang
Z
Z
Yang
H
H
Cao
W
W
Duan
J
J
D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004586:Electrophoresis; D014644:Genetic Variation; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012241:Rhodopseudomonas / Q000145:classification* / Q000235:genetics*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S