title: [Antibacterial and antifungal effects of Agkistrodon halys Pallas: purification of its antibacterial protein--LAO].
abstract: This paper reports the venom from Agkistrodon halys Pallas have inhibitory activity against fungi and E. coli by tested in a disc diffusion assay. An antibacterial component--LAO from the venom were purified to homogeneous. It had not only antibacterial effect, but L-amino acid oxidase activity. And its enzymatic specific activity was 808 U/mg. The venom had at least 3 antibacterial components (I, II, III) as determined by acid polyacrilamide gel electrophoresis, LAO is the antibaterial components II.
authors.affiliation: College of Life Science, Nanjing Normal University, Nanjing 210097.
authors.forename: X M
authors.initials: XM
authors.lastname: Yan
authors.forename: S Q
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Q
authors.initials: Q
authors.lastname: Chang
authors.forename: P
authors.initials: P
authors.lastname: Liu
authors.forename: J S
authors.initials: JS
authors.lastname: Xu
mesh_terms: D017836:Agkistrodon; D000594:Amino Acid Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D003435:Crotalid Venoms / Q000201:enzymology / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects*; D005658:Fungi / Q000187:drug effects*; D050830:L-Amino Acid Oxidase
chemical_list: D000890:Anti-Infective Agents; D003435:Crotalid Venoms; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase