[Preparation of novel chiral stationary phases for ligand exchange chromatography and their applications].
Two novel chiral stationary phases for ligand exchange chromatography were prepared by synthesizing 2-(2-hydroxyl-3-octoxy)propyl-(S)-1, 2, 3, 4-tetrahydro-3-isoquinolinecarboxylic acid (C8-(S)-THIQCA) and 2-(2-hydroxyl-3-dodecoxy)propyl-(S)-1, 2, 3, 4-tetrahydro-3-isoquinolinecarboxylic acid (C12-(S)-THIQCA) as chiral selectors, and coating them on ODS bonded stationary phase. Chromatographic resolutions of some DL-amino acids were achieved on the chiral stationary phases by using 1 mmol/L NH4Cl with 0.2 mmol/L Cu(Ac)2 solution as mobile phase and detection at 254 nm. Ability of chiral resolution of the DL-amino acids through the stationary phases was compared and the effect of cupric ion on resolutions of the DL-amino acids was examined. The elution order of D-isomer before L-isomer was observed for all the DL-amino acids resolved, except DL-Val. For DL-Pro, DL-Val and DL-Leu the elution order through the chiral phases was different from that through the chiral ligand exchange phases prepared from L-Pro or L-hydroxyl proline with five membered ring structure.
Wang
Q B
QB
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, China.
Long
Y D
YD
Huang
T B
TB
D000596:Amino Acids / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D007546:Isoquinolines; D008024:Ligands; D013237:Stereoisomerism
D000596:Amino Acids; D007546:Isoquinolines; D008024:Ligands; C039109:isoquinoline