title: [Genetic identification of the Crimean-Congo hemorrhagic fever virus during epidemic outbreak in Kazakhstan in 2000].
abstract: Sera samples from patients suspected of Crimean-Congo hemorrhagic fever (CCHF) taken during epidemic outbreak at the territory of Sarysusky and Moiynkumsky districts of the Zhambyl region in Kazakhstan, in 2000, were analysed by means of reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of virus genome fragments. Genome RNA of CCHF virus was found in 2 assays. Analysis of nucleotide sequences of fragments of S-segment of viral genome revealed in the Sarysusky districts circulation of CCHF virus, genetically resembled to close phylogenetically to CCHF virus strains from China.
authors.affiliation: State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Region, Russia.
authors.forename: L N
authors.initials: LN
authors.lastname: Iashina
authors.forename: V S
authors.initials: VS
authors.lastname: Petrov
authors.forename: I D
authors.initials: ID
authors.lastname: Petrova
authors.forename: V V
authors.initials: VV
authors.lastname: Gutorov
authors.forename: S V
authors.initials: SV
authors.lastname: Kazakov
authors.forename: K S
authors.initials: KS
authors.lastname: Ospanov
authors.forename: S K
authors.initials: SK
authors.lastname: Karimov
authors.forename: G I
authors.initials: GI
authors.lastname: Tiunnikov
authors.forename: S V
authors.initials: SV
authors.lastname: Seregin
authors.forename: I I
authors.initials: II
authors.lastname: Kuzina
authors.forename: I V
authors.initials: IV
authors.lastname: Babkin
authors.forename: S V
authors.initials: SV
authors.lastname: Netesov
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D002681:China; D004196:Disease Outbreaks; D014644:Genetic Variation; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000235:genetics* / Q000302:isolation & purification; D006479:Hemorrhagic Fever, Crimean / Q000453:epidemiology* / Q000821:virology*; D006801:Humans; D007623:Kazakhstan / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid