The study of chemiluminescence in gastric and colonic carcinoma cell lines treated by anti-tumor drugs.
OBJECTIVE
To study the influence of chemotherapy on proliferation activation of tumor cell by observing the change of chemiluminescence (CL) and cell cycle in various tumor cell lines after mitomycin C treated.


METHODS
BGC823 and LoVo cell lines were all cultured in RPMI-1640, and then were adjusted to a concentration of 1X10(5) cells/ml in fresh media and incubated for 24 h. Mitomycin C (100 ng.ml(-1)) was added to each bottle. All indeses were examined after 24 h. No Mitomycin C was added in control group. Each group contained 8 samples. Flow cytometric analysis and luminol-dependent CL were used to investigate the effect of mitomycin C on two gastrointestinal carcinoma cell lines.


RESULTS
BGC823 and LoVo cell lines incubated with MMC for 24 h. We discovered that the emergence of peak of CL stimulated by PHA was postponed significantly (BGC823: 12.63+/-3.21 vs 4.50+/-1.04, LoVo: 13.25+/-2.96 vs 5.12+/-1.36, P<0.01) and the peak intension of CL was reduced significantly (BGC823: 120.25+/-16.61 vs 248.38+/-29.17, LoVo: 98.13+/-10.49 vs 267.50+/-18.56, P<0.01). The PI of cell lines was decreased significantly (BGC823: 51.87+/-4.82 vs 25.44+/-2.26, LoVo: 47.11+/-1.04 vs 24.23+/-0.37, P<0.01) and the apoptotic fractions changed by contraries (BGC823: 26.25+/-5.29 vs 9.83+/-2.51, LoVo: 33.50+/-3.68 vs 9.63+/-1.44, P<0.01).


CONCLUSIONS
CL can be used to measure activation of tumor cells. We discovered that the ground CL intensions of two cell lines were not high but increased rapidly after stimulation of PHA. The CL peak ranged from 4-5 minutes, and then decreased gradually. The results were not reported before. CL of tumor cell has close correlativity with the dynamics of cell cycle and can reflect the feature of oxidation metabolism and proliferation activation of tumor cell. So it can be used to observe the influence of chemotherapy drug on metabolism and proliferation activation of tumor cell and screen out chemotherapy drugs to which tumor cells are sensitive.
Chen
Che
C
Department of General Surgery, Jinling Hospital, 305 Zhongshandong Road, Nanjing 210002, China. drchenche@sohu.com
Liu
Fu-Kun
FK
Qi
Xiao-Ping
XP
Li
Jie-Shou
JS
D000903:Antibiotics, Antineoplastic / Q000627:therapeutic use*; D002453:Cell Cycle / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D008163:Luminescent Measurements; D016685:Mitomycin / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology; D014407:Tumor Cells, Cultured
D000903:Antibiotics, Antineoplastic; D016685:Mitomycin