[The correlation of multidrug resistance phenotype with clinical response to chemotherapy in laryngeal cancer].
OBJECTIVE
The correlation of multidrug resistance(MDR) phenotype with clinical response to chemotherapy was investigated in patients with laryngeal cancer.


METHODS
Tumor specimens prior to neoadjuvant chemotherapy from 36 cases of laryngeal cancer were collected for detection of P-glycoprotein (P-gp) with anti-Pgp monoclonal antibodies (JSB-I). Immunohistochemical assays were used for P-gp detection on 5 microns thick frozen section. Anti-Pgp monoclonal antibodies(JSB-I) as primary antibodies and goat anti-mouse IgG monoclonal antibodies as second antibodies were applied. All patients received preoperative induction chemotherapy with a regimen of cisplatin, 5-fluorouracil and pingyangmycin. Evaluation for tumor response was scored as follows: Response to chemotherapy was graded as good for complete response or partial response; as poor for stable disease or progressive disease.


RESULTS
Of the 36 pretreatment specimens, 12(33.3%) were P-gp positive and 24 (66.7%) were P-gp negative. Among the 12 patients with P-gp positive, 3(25%) had a good response and 9(75%) had a poor response. Of the 24 patients with P-gp negative, 21(87.5%) had a good response and 3(12.5%) had a poor response (P < 0.05).


CONCLUSIONS
These preliminary data suggest that a significant correlation between MDR phenotype, Pgp-mediated and chemotherapy resistance existed.
Li
X
X
Department of Otolaryngology, Baoan People's Hospital, Shenzhen 518101.
Yang
C
C
Su
Z
Z
Song
Y
Y
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis*; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy*; D018432:Drug Resistance, Multiple / Q000235:genetics*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy*; D008297:Male; D008875:Middle Aged; D010641:Phenotype
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1