title: [Recombinant Salmonella typhimurium anticaries vaccine. II. Construction and confirmation of expressing plasmid carrying a region of S. mutans pac gene].
abstract: OBJECTIVE
  The objective of this study is to construct prokaryotic expressing plasmids carrying A region of S. mutans pac gene.
  
  
  METHODS
  The A region of pac gene was amplified by using PCR from plasmid pPC41 containing S. mutans surface protein pac gene. The new expression plasmid was constructed by using A region of pac gene and expressing plasmids pET17-b and directed cloning technique. The recombinant plasmid was identified by agarose gel electrophoresis after it was cut by using BamHI and EcoRV.
  
  
  RESULTS
  The expressing plasmid carrying pac gene A region was acquired.
  
  
  CONCLUSIONS
  The recombinant plasmid provides useful experimental materials for further research work.
authors.affiliation: College of Stomatology, Sun Yat-sun University of Medical Sciences.
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Ling
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.forename: J
authors.initials: J
authors.lastname: Tu
mesh_terms: D003731:Dental Caries / Q000517:prevention & control*; D015870:Gene Expression; D005798:Genes, Bacterial; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic; D022562:Salmonella Vaccines / Q000235:genetics* / Q000276:immunology; D012486:Salmonella typhimurium / Q000235:genetics* / Q000276:immunology; D013295:Streptococcus mutans / Q000235:genetics*
chemical_list: D022562:Salmonella Vaccines