title: Eye opening induces a rapid dendritic localization of PSD-95 in central visual neurons.
abstract: The membrane-associated guanylate kinase PSD-95 scaffolds N-methyl-d-aspartate receptors to cytoplasmic signaling molecules, and associates with other glutamate receptors at central synapses. However, regulation of PSD-95 in vivo is poorly understood. We provide evidence of an activity-dependent redistribution of PSD-95 to dendrites in central visual neurons that is tied to eye opening. Six hours after eye opening, increased dendritic PSD-95 coimmunoprecipitates with the same proportions of stargazin, increased proportions of the N-methyl-d-aspartate receptor subunit NR2A, and decreased proportions of NR2B. Sustained high levels of PSD-95 in dendrites are dependent on continued pattern vision in juvenile but not mature animals, suggesting that the stabilization of PSD-95 at synapses may be involved in the control of developmental plasticity.
authors.affiliation: Department of Biology, Center for Learning and Memory, McGovern Institute for Brain Research, and Howard Hughes Medical Institute, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139-4307, USA.
authors.forename: Akira
authors.initials: A
authors.lastname: Yoshii
authors.forename: Morgan H
authors.initials: MH
authors.lastname: Sheng
authors.forename: Martha
authors.initials: M
authors.lastname: Constantine-Paton
mesh_terms: D000818:Animals; D003712:Dendrites / Q000378:metabolism*; D000076122:Disks Large Homolog 4 Protein; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D018613:Microscopy, Confocal; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D009900:Optic Nerve / Q000378:metabolism* / Q000502:physiology*; D011233:Precipitin Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism; D013997:Time Factors
chemical_list: D000076122:Disks Large Homolog 4 Protein; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D016194:Receptors, N-Methyl-D-Aspartate; C501449:Dlg4 protein, rat; C121001:NR2B NMDA receptor; C033652:postsynaptic density proteins