Lipoarabinomannan-reactive human secretory immunoglobulin A responses induced by mucosal bacille Calmette-Guérin vaccination.
The ability of 17 recombinant mycobacterial proteins, native antigen 85 complex, lipoarabinomannan (LAM), and Mycobacterium tuberculosis lysate to detect antibody responses induced by bacille Calmette-Guérin (BCG) vaccination and active tuberculosis infection were studied in enzyme-linked immunosorbent assays. Only LAM-reactive serum immunoglobulin G responses were significantly increased in both BCG-vaccinated patients and patients with active tuberculosis (P<.05), and oral BCG vaccination also induced significant increases in LAM-reactive secretory immunoglobulin A (P<.05). LAM-reactive antibody assays can serve as markers of humoral and mucosal immunity in future trials of BCG and newer attenuated mycobacterial vaccines.
Brown
Robin M
RM
Saint Louis University Vaccine and Treatment Evaluation Unit, Division of Infectious Diseases and Immunology, Department of Internal Medicine, Saint Louis University, St. Louis, Missouri 63110, USA.
Cruz
Orlando
O
Brennan
Michael
M
Gennaro
Maria L
ML
Schlesinger
Larry
L
Skeiky
Yasir A W
YA
Hoft
Daniel F
DF
D000293:Adolescent; D000328:Adult; D001500:BCG Vaccine / Q000276:immunology*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D014611:Vaccination
D001500:BCG Vaccine; D007070:Immunoglobulin A; D008070:Lipopolysaccharides; C050016:lipoarabinomannan