The induction of baboon glycodelin expression by progesterone is not through Sp1.
Glycodelin is a major secretory product of the uterine glandular epithelial cells of the human and non-human primate during the late luteal phase of the menstrual cycle and early pregnancy. Since progesterone levels are elevated during these periods we sought to determine how progesterone modulates glycodelin gene expression. Co-transfection of various deletions of the baboon glycodelin promoter with the progesterone receptor (PR) into Ishikawa cells, a human endometrial cell line, revealed that full progesterone responsiveness is retained within the region -119/+48. In COS-1 cells, a kidney cell line, progesterone failed to elevate luciferase levels when various deletion constructs and the PR were co-transfected. Mutation of the Sp1 site in the -67/+48 region lowered basal expression but did not affect the ability of progesterone to increase expression of the luciferase reporter in Ishikawa cells. These findings suggest that Sp1 sites are not involved in the progesterone regulation of the baboon glycodelin gene. We propose that progesterone induces a factor that regulates glycodelin gene expression in the uterus since we failed to obtain a similar response in a non-uterine cell line.
Jaffe
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RC
Department of Physiology and Biophysics, University of Illinois at Chicago, 60612, USA. rcjaffe@uic.edu
Donnelly
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KM
Fazleabas
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D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000187:drug effects; D015698:Genomic Library; D000071816:Glycodelin; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007668:Kidney; D008297:Male; D008969:Molecular Sequence Data; D010215:Papio; D011257:Pregnancy Proteins / Q000235:genetics*; D011374:Progesterone / Q000494:pharmacology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D016329:Sp1 Transcription Factor / Q000378:metabolism; D016385:TATA Box; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection
D000071816:Glycodelin; D006023:Glycoproteins; C045246:PAEP protein, human; D011257:Pregnancy Proteins; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D011374:Progesterone