[The characterization of pJW566 from L. Lactis subsp. cremoris W56].
The plasmid pJW566 was isolated from L. lactis subsp. cremoris W56, one strain for Danish chadder mixed starter cultures. The strain containing plasmid pJW566 showed resistance against three common phages species 936, c2 and P335 worldwide. It was found that pJW566 encoded for an restriction and modification system, and showed strong resistance to phage CHCP412 when it was introduced into the industrial strain L. lactis CHCC2281 in milk medium. The endonuclease activity analysis indicated that the endonuclease required Mg2+, ATP, and was stimulated by AdoMet.
Kong
J
J
State Key Lab of Microbial Technology, Shandong University, Jinan 250100, China.
Gu
X
X
Ma
G
G
D000818:Animals; D001435:Bacteriophages / Q000472:pathogenicity*; D015280:DNA Restriction-Modification Enzymes / Q000235:genetics*; D018274:Electroporation; D004720:Endonucleases / Q000378:metabolism; D013294:Lactococcus lactis / Q000201:enzymology / Q000235:genetics / Q000821:virology*; D008892:Milk / Q000378:metabolism; D010957:Plasmids; D012436:S-Adenosylmethionine / Q000494:pharmacology
D015280:DNA Restriction-Modification Enzymes; D012436:S-Adenosylmethionine; D004720:Endonucleases