Broad-range ribosomal RNA real-time PCR after removal of DNA from reagents: melting profiles for clinically important bacteria.
Tseng
Ching-Ping
CP
School of Medical Technology and Graduate Institute of Medical Biotechnology, Chang Gung University, Tao-Yuan 333, Taiwan, Republic of China.
Cheng
Ju-Chien
JC
Tseng
Ching-Chung
CC
Wang
Cheyien
C
Chen
Yi-Ling
YL
Chiu
Daniel Tsun-Yee
DT
Liao
How-Chin
HC
Chang
Shy-Shin
SS
D052160:Benzothiazoles; D004269:DNA, Bacterial / Q000302:isolation & purification*; D003850:Deoxyribonuclease I; D003959:Diamines; D004926:Escherichia coli / Q000235:genetics; D005456:Fluorescent Dyes / Q000302:isolation & purification*; D007202:Indicators and Reagents / Q000302:isolation & purification*; D009930:Organic Chemicals; D016133:Polymerase Chain Reaction; D011804:Quinolines; D012329:RNA, Bacterial / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000032:analysis*
D052160:Benzothiazoles; D004269:DNA, Bacterial; D003959:Diamines; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009930:Organic Chemicals; D011804:Quinolines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C098022:SYBR Green I; D003850:Deoxyribonuclease I