Degradation of polyvinyl alcohol by Sphingomonas sp. SA3 and its symbiote.
A total of 800 samples was taken from Taegu province, Korea, where many textile factories provide a source of polyvinyl alcohol (PVA) waste. These samples were screened for PVA-degrading bacteria. A new strain, SA3, was discovered which formed yellow colonies and used PVA as the sole carbon and energy source. Strain SA3 was identified as a Sphingomonas sp., based on the partial nucleotide sequence analysis of 16S ribosomal RNA, the presence of 2-hydroxymyristic acid (14:O 2-OH) and sphingolipids with d-17:0, d-18:0, d-19:1, and d-20:1 as the main dihydrosphingosines. This genus has not previously been reported as a PVA-degrading bacterium. Sphingomonas sp. SA3 needs a symbiote strain, SA2, for PVA degradation as a growth factor producer. In mixed cultures of these strains, the optimum temperature for PVA biodegradation ranged from 30 degrees C to 35 degrees C. The optimum pH was 8.0 and the most effective nitrogen source was NH(4)(+).
Kim
B C
BC
College of Natural Sciences, Kyungpook National University, Department of Microbiology, 702-701, Taegu, Korea.
Sohn
C K
CK
Lim
S K
SK
Lee
J W
JW
Park
W
W
D001673:Biodegradation, Environmental; D003470:Culture Media; D004275:DNA, Ribosomal / Q000032:analysis; D004783:Environmental Microbiology; D007723:Korea; D008969:Molecular Sequence Data; D011142:Polyvinyl Alcohol / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D020578:Sphingomonas / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D013559:Symbiosis
D003470:Culture Media; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D011142:Polyvinyl Alcohol