Mucin gene expression in the effusions of otitis media with effusion.
OBJECTIVE
The purpose of the study is to know if mucin gene expression can be detected in the middle ear effusion and if so, which mucin genes are expressed in the effusions.


METHODS
Mucin gene expression in the middle ear effusions obtained from five patients with otitis media with effusion were analyzed by reverse transcription-polymerase chain reaction. Ribonucleic acids (RNAs) were extracted from the effusion and the expression of 12 mucin genes was analyzed by reverse transcription-polymerase chain reaction.


RESULTS
Mucin gene expression examined by reverse transcription-polymerase chain reaction indicated the expression of MUC1, MUC4, MUC5AC, MUC6, MUC7, MUC8, MUC9, MUC11 and MUC12 mRNA in the effusion. This mucin gene expression was similar to that in BEAS-2B cell, a bronchial epithelial cell line.


CONCLUSIONS
Middle ear effusion can give us valuable information on mucin gene expression in the middle ear. There is similarity between mucin gene expression in the middle ear effusion and that in the bronchial epithelia.
Takeuchi
Kazuhiko
K
Department of Otorhinolaryngology, Mie University School of Medicine, 2-174 Edobashi Tsu, Mie 514-8507, Japan. kazuhiko@clin.medic.mie-u.ac.jp
Yagawa
Mayuko
M
Ishinaga
Hajime
H
Kishioka
Chikako
C
Harada
Teruhiko
T
Majima
Yuichi
Y
D000328:Adult; D000368:Aged; D002648:Child; D017931:DNA Primers / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D055271:Mucin 5AC; D055264:Mucin-4; D055272:Mucin-6; D009077:Mucins / Q000235:genetics*; D010034:Otitis Media with Effusion / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D006023:Glycoproteins; C524658:MUC4 protein, human; C524678:MUC5AC protein, human; C524734:MUC6 protein, human; C105059:MUC8 protein, human; D008565:Membrane Proteins; D055271:Mucin 5AC; D055264:Mucin-4; D055272:Mucin-6; D009077:Mucins; C091468:OVGP1 protein, human