[Studies on the effects of astrocyte during glial scar formation by recombinant antisense GFAP retrovirus].
The present study was to investigate the changes of macroglia and the effect of gonadal hormone on reactive gliosis and of antisense GFAP retrovirus on astrocyte, gliosis and glial scar formation after brain stab injury (BSI). The results indicate that astrocytes are the main cells of glial scar. GFAP plays an important role in the maintenance of structure and function of astrocytes. The oligodendrocyte is not an active cell during glial scar formation. The gonadal hormone can modify the reactivity of astrocyte after BSI, but has no significant effect on differentiation and proliferation of astrocyte. The recombined GFAP retrovirus can effectively inhibit the growth of astrocyte, and GFAP expression of injured astrocyte in vitro and glial scar forming in vivo.
Huang
Q L
QL
Department of Neurosurgery, Xingqiao Hospital, Third Military Medical University, Chongqing 400037.
D000818:Animals; D016375:Antisense Elements (Genetics) / Q000378:metabolism; D001253:Astrocytes / Q000473:pathology; D001930:Brain Injuries / Q000378:metabolism / Q000503:physiopathology*; D002921:Cicatrix / Q000473:pathology*; D005822:Genetic Vectors / Q000235:genetics; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D009457:Neuroglia / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012190:Retroviridae / Q000235:genetics
D016375:Antisense Elements (Genetics); D005904:Glial Fibrillary Acidic Protein; D011994:Recombinant Proteins