title: Age-related alterations in the protein expression profile of C57BL/6J mouse pituitaries.
abstract: The aim of our study was to monitor the protein expression profile in pituitary glands of healthy C57BL/6J mice during aging. Pituitary glands of 4-week old (immature), 3-month old (mature), and >25-month old mice were analysed by proteomic tools such as two-dimensional electrophoresis and N-terminal micro-sequencing. A change was detected in the expression of growth hormone after sexual maturation. Our particular interest, however, was directed against up-regulated proteins in the old pituitary glands, which are proposed to be involved in the process of neuroendocrine aging. Among these proteins, the expression of glutathione-S-transferase (GST) and apolipoprotein A-1 were increased in old pituitaries. Furthermore, ubiquitin carboxyl-terminal hydrolase (UCH-L1) was significantly up-regulated in senescent C57BL/6J mouse pituitaries. Since only the rat homologue was known, we isolated and analysed the mouse UCH-L1 sequence. Since GST is involved in antioxidative defence and UCH-L1 is part of the ubiquitin/proteasome system, which is responsible for the removal of damaged proteins, these results suggest increased oxidative burden and an increased activity of the ubiquitin system.
authors.affiliation: Institute of Applied Microbiology, University of Agricultural Sciences, Muthgasse 18, House B, A-1190 Vienna, Austria. g.marzban@iam.boku.ac.at
authors.forename: Gorji
authors.initials: G
authors.lastname: Marzban
authors.forename: Johannes
authors.initials: J
authors.lastname: Grillari
authors.forename: Eva
authors.initials: E
authors.lastname: Reisinger
authors.forename: Thomas
authors.initials: T
authors.lastname: Hemetsberger
authors.forename: Reingard
authors.initials: R
authors.lastname: Grabherr
authors.forename: Hermann
authors.initials: H
authors.lastname: Katinger
mesh_terms: D000375:Aging / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D016632:Apolipoprotein A-I / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D005982:Glutathione Transferase / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D013869:Thiolester Hydrolases / Q000378:metabolism; D043222:Ubiquitin Thiolesterase; D015854:Up-Regulation / Q000502:physiology
chemical_list: D016632:Apolipoprotein A-I; D011506:Proteins; D005982:Glutathione Transferase; D013869:Thiolester Hydrolases; D043222:Ubiquitin Thiolesterase