[Promotion of transformation frequency of soybean (Glycine max L.) protoplasts using poly-L-ornithine].
The foreign Bt gene was transferred into protoplasts of soybean using PEG and PLO methods, respectively. The result indicated that the transformation frequency of PLO method was about 0.1% higher than PEG method. The PCR and Southern blotting analysis of the regeneration plants confirmed the integration of foreign gene into the genome of soybean.
Nan
X R
XR
Biotechnology Research Center, Heilongjiang Academy of Agriculture Science, Harbin 150086.
Wei
Z M
ZM
D010455:Peptides / Q000494:pharmacology*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D011523:Protoplasts / Q000187:drug effects*; D013025:Glycine max / Q000187:drug effects / Q000235:genetics*; D014170:Transformation, Genetic
D010455:Peptides; C008973:polyornithine