Quantitative intra-individual monitoring of BCR-ABL transcript levels in archival bone marrow trephines of patients with chronic myeloid leukemia.
We established a quantitative real-time RT-PCR assay for the detection of chimeric BCR-ABL transcripts in archival formalin-fixed bone marrow trephines, both acrylate-embedded and paraffin-embedded. This new methodology enables determination of transcript levels in direct comparison to histopathological findings and therapeutic interventions during the time course of the disease in a retrospective and a prospective manner. We found an excellent correlation between the quantitative molecular data and the morphological evaluation as well as the clinical outcome for a cohort of chronic myeloid leukemia patients (n = 10). To the best of our knowledge, this is the first study demonstrating the feasibility of large-scale quantitative expression analysis in archival bone marrow trephines for monitoring molecular markers over several years or even decades.
Bock
Oliver
O
Institute of Pathology, Medizinische Hochschule Hannover, Hannover, Germany. Bock.Oliver@MH-Hannover.de
Lehmann
Ulrich
U
Kreipe
Hans
H
D001483:Base Sequence; D001853:Bone Marrow / Q000378:metabolism; D015331:Cohort Studies; D017931:DNA Primers / Q000235:genetics; D016313:Genes, abl; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D025202:Molecular Diagnostic Techniques; D011379:Prognosis; D012334:RNA, Neoplasm / Q000032:analysis / Q000235:genetics; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D016610:Tissue Embedding; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D012334:RNA, Neoplasm