Vesicle budding: a coat for the COPs.
Although vesicular transport in eukaryotic cells involves a number of different carriers, one common feature is that most of them use small GTPases to direct coat assembly at the donor membrane. COPII coated vesicles bud from the endoplasmic reticulum to selectively export secretory cargo en route to the Golgi complex. Vesicle formation involves the stepwise recruitment of the small GTPase Sar1 and two large heterodimeric complexes Sec23-Sec24 and Sec13-Sec31 to the membrane. A new structural study now provides breathtaking molecular insights into the formation of the Sec23-Sec24-Sar1 pre-budding complex and into COPII coat assembly.
Haucke
Volker
V
Zentrum Biochemie and Molekulare Zellbiologie, University of Göttingen, Humboldtallee 23, D-37073 Göttingen, Germany. vhaucke@gwdg.de
D000818:Animals; D022181:COP-Coated Vesicles / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000378:metabolism*; D020690:GTPase-Activating Proteins; D006801:Humans; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins; D020836:Protein Structure, Quaternary / Q000502:physiology; D021381:Protein Transport / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D033921:Vesicular Transport Proteins
D020690:GTPase-Activating Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D028861:Nuclear Pore Complex Proteins; C079620:SEC13 protein, S cerevisiae; C079037:SEC23 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; D020559:Monomeric GTP-Binding Proteins; C077395:SAR1 protein, S cerevisiae