title: [Purification of follicle-stimulating hormone by radial-flow chromatography].
abstract: By comparing the purification results of radial-flow chromatography with conventional chromatography, a new purification process of follicle-stimulating hormone (FSH) from crude sample of human menopausal gonadotrophin has been developed. The results showed that the specific activity, activity recovery, and purification factor of FSH purified by radial-flow chromatography were higher than those by conventional chromatography in large scale. The specific activity of FSH purified was 180 IU/mg protein, activity recovery 56%, purification factor 150. The purified product meets the quality requirement of the Standard of Chinese Pharmacopoeia.
authors.affiliation: Central Labaratory, 4th Military Medical University, Xi'an 710032, China. lianguo@fmmu.edu.cn
authors.forename: L A
authors.initials: LA
authors.lastname: Guo
authors.forename: Z
authors.initials: Z
authors.lastname: Yan
authors.forename: X D
authors.initials: XD
authors.lastname: Yang
authors.forename: X Q
authors.initials: XQ
authors.lastname: Chu
authors.forename: H F
authors.initials: HF
authors.lastname: Xu
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods*; D005640:Follicle Stimulating Hormone / Q000302:isolation & purification*; D008596:Menotropins
chemical_list: D008596:Menotropins; D005640:Follicle Stimulating Hormone