title: Combinatorial peptide library methods for immunobiology research.
abstract: The field of combinatorial peptide chemistry has emerged as a powerful tool in the study of many biological systems. This review focuses on combinatorial peptide library methodology, which includes biological library methods, spatially addressable parallel library methods, library methods requiring deconvolution, the "one-bead one-compound" library method, and affinity chromatography selection method. These peptide libraries have successfully been employed to study a vast array of cell surface receptors, as well as have been useful in identifying protein kinase substrates and inhibitors. In recent immunobiological applications, peptide libraries have proven monumental in the definition of MHC anchor residues, in lymphocyte epitope mapping, and in the development of peptide vaccines. Peptides identified from such libraries, when presented in a chemical microarray format, may prove useful in immunodiagnostics. Combinatorial peptide libraries offer a high-throughput approach to study limitless biological targets. Peptides discovered from such studies may be therapeutically and diagnostically useful agents.
authors.affiliation: UC Davis Cancer Center, Division of Hematology/Oncology, and Department of Internal Medicine, University of California Davis, Sacramento, CA, USA.
authors.forename: Ruiwu
authors.initials: R
authors.lastname: Liu
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Enstrom
authors.forename: Kit S
authors.initials: KS
authors.lastname: Lam
mesh_terms: D000818:Animals; D001435:Bacteriophages; D002846:Chromatography, Affinity; D020650:Combinatorial Chemistry Techniques / Q000295:instrumentation; D015195:Drug Design; D004791:Enzyme Inhibitors / Q000302:isolation & purification; D018604:Epitope Mapping / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007158:Immunologic Techniques; D008024:Ligands; D008285:Major Histocompatibility Complex / Q000276:immunology; D008863:Microspheres; D019151:Peptide Library; D014612:Vaccines
chemical_list: D004791:Enzyme Inhibitors; D008024:Ligands; D019151:Peptide Library; D014612:Vaccines