Overlapping deletions define novel embryonic lethal loci in the mouse t complex.
The t complex region of mouse chromosome 17 contains genetic information critical for embryonic development. To identify and map loci required for normal embryogenesis, a set of overlapping deletions (D17Aus9(df10J), D17Aus9(df12J), and D17Aus9(df13J)) surrounding the D17Aus9 locus and one encompassing the T locus, Del(17)T(7J), were bred in various combinations and the consequences of nullizygosity in overlapping regions were examined. The results indicated that there are at least two functional units within 1 cM of D17Aus9. l17J1 is a peri-implantation lethal mutation within the region deleted in D17Aus9(df13J), whereas l17J2 is a later-acting lethal defined by the region of overlap between Del(17)T(7J) and D17Aus9(df12J). Del(17)T(7J)/D17Aus9(df12J) embryos die around 10.5 dpc. The development of the mutant embryos is characterized by lack of axial rotation, an abnormal notochord structure, and a ballooning pericardium. These studies demonstrate the value of overlapping deletion complexes, as opposed to individual deletion complexes, for the identification, mapping, and analysis of genes required for embryonic development.
Chao
Hanna H J
HH
Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6445, USA.
Mentzer
Sarah E
SE
Schimenti
John C
JC
You
Yun
Y
D000483:Alleles; D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D003433:Crosses, Genetic; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D017403:In Situ Hybridization; D051379:Mice; D008957:Models, Genetic; D009154:Mutation; D009672:Notochord / Q000378:metabolism; D013997:Time Factors