title: T lymphocyte subpopulations and B lymphocyte cells in caecum and spleen of chicks infected with Salmonella enteritidis.
abstract: The effect of low and high doses of Salmonella enteritidis PT4 (SE) on immunocompetent cells in caecum and spleen of one-day-old chicks was investigated. Subsets of T lymphocytes positive for CD3, CD4, CD8 and B lymphocytes (Bu1b-positive cells) were counted in the caecum after immunohistochemical staining and the relative percentage of these cells in the spleen was analysed using a FACScan cytometer on days 7, 10, 14, 21, and 27 post-inoculation (pi). In the low dose group, the number of CD3+ and CD4+ cells in the caecum had significantly increased at day 10 pi. Both CD8+ and Bu1b+ cells were significantly higher on day 14 pi in this group. In the high-dose group, the number of CD4+ cells had significantly increased at day 7 pi. CD3+, CD8+, and Bu1b+ cells showed prolonged proliferation at days 7 up to 21 pi. Splenic lymphocytes demonstrated significant changes only in the high dose group. The percentage of splenic CD4+ cells was decreased at day 7 pi. A decrease in CD3+ and CD8+ cells was found at day 14 pi in this group.
authors.affiliation: Institute of Pathological Anatomy, University of Veterinary Medicine, Kosice, Slovak Republic. asheg@uvm.sk
authors.forename: Abdulatif
authors.initials: A
authors.lastname: Asheg
authors.forename: Mikulás
authors.initials: M
authors.lastname: Levkut
authors.forename: Viera
authors.initials: V
authors.lastname: Revajová
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Sevcíková
authors.forename: Lev
authors.initials: L
authors.lastname: Kolodzieyski
authors.forename: Juraj
authors.initials: J
authors.lastname: Pistl
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D002432:Cecum / Q000276:immunology* / Q000473:pathology; D002452:Cell Count; D002645:Chickens; D004195:Disease Models, Animal; D005434:Flow Cytometry; D007124:Immunoenzyme Techniques; D011201:Poultry Diseases / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D012481:Salmonella Infections, Animal / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D012477:Salmonella enteritidis / Q000472:pathogenicity / Q000502:physiology; D013154:Spleen / Q000276:immunology* / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism / Q000473:pathology
chemical_list: D015703:Antigens, CD