[Analysis of trehalose in transgenic tobacco extracts by high performance liquid chromatography with evaporative light scattering detection].
A high performance liquid chromatographic method with ethylenediamine dynamically modified silica column and evaporative light-scattering detection (ELSD) was developed for the direct determination of trehalose in transgenic tobacco extracts. The separation column (Zorbax Rx-SIL, 4.6 mm i.d. x 250 mm, 5 microns, Hewlett-Packard, USA) modified by ethylenediamine and a guard column (Zorbax Rx-SIL, 4.6 mm i.d. x 12.5 mm, 5 microns) were used. The mobile phase was a mixture of acetonitrile-water(2.6:1, V/V) containing 0.03% ethylenediamine. Regression equations revealed the linear relationships (r = 0.996-0.999) between the concentrations and peak areas of carbohydrates detected by ELSD. The detection limits of ELSD (S/N = 3:1) were 10 mg/L, 20 mg/L, 10 mg/L and 10 mg/L for fructose, glucose, sucrose and trehalose, respectively.
Wei
Y
Y
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Ding
M Y
MY
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005818:Genetic Engineering; D030821:Plants, Genetically Modified; D012542:Scattering, Radiation; D012822:Silicon Dioxide; D014026:Nicotiana / Q000235:genetics*; D014199:Trehalose / Q000032:analysis / Q000235:genetics*
D012822:Silicon Dioxide; D014199:Trehalose