[Simultaneous determination of sulbactam sodium and cefoperazone sodium in sulperazon by high performance liquid chromatography].
A reversed-phase, isocratic high performance liquid chromatographic method with acid mobile phase can separate sulbactam and cafoperazone within 12 minutes. Column packed with Hypersil ODS2(250 mm x 4.6 mm i.d., 5 microns) was manufactured by Dalian Elite Company. Mobile phase is composed of water (adjusted to pH 4.0 with 1% phosphoric acid) and acetonitrile (80:20, V/V). The detection was performed at 210 nm and the injection volume was 2 microL. Cefoperazone and sulbactam have good linearity in the ranges of 100 mg/L to 800 mg/L and 100 mg/L to 1,000 mg/L with the correlation coefficients of 0.9991 and 0.9997 respectively. This method is easily to be operated and can be applied for manufacturing and medicinal study.
Li
F S
FS
Department of Clinical Laboratory, Dalian Medicinal University, Dalian 116027, China.
Xu
Z X
ZX
Xiao
H B
HB
Liang
X M
XM
D000892:Anti-Infective Agents, Urinary / Q000737:chemistry; D002438:Cefoperazone / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D013407:Sulbactam / Q000032:analysis* / Q000737:chemistry*
D000892:Anti-Infective Agents, Urinary; D004338:Drug Combinations; D002438:Cefoperazone; C057923:sulperazone; D013407:Sulbactam