[Expression of matrix metalloproteinases in metastatic lesions of cutaneous squamous cell carcinoma].
OBJECTIVE
To investigate the expression of matrix metalloproteinases (MMPs) and its correlation with basigin/CD147 expression in metastatic lesions of cutaneous Sequamous cell carcinoma (SCC).


METHODS
Expressions of MMPs and basigin/CD147 were examined by DAB and ALP immunohistochemical staining in 14 metastatic SSCs, and immunoblot analysis was used to observe the basigin/CD147 expression in metastatic samples.


RESULTS
Normal skin and lymph nodes were basically negative for MMPs, but expressions of MMP-1, MMP-2, MMP-3 and MTl-MMP were increased in both tumor cells and stromal cells in metastatic SCC. Strongly positive or positive staining was observed in 4 or 8 of metastatic SCCs for basigin/CD147 in tumor cells respectively. Significant correlations were found between basigin/CD147 expression in tumor cells and MMP-1, MMP-2, MMP-3, MTl-MMP expression in surrounding stromal cells (p-values: 0.012, 0.024, 0.047 and 0.026, respectively). Immunoblot analysis revealed that basigin/CD147 expression increased in metastatic SCC.


CONCLUSIONS
These results suggest that MMPs produced by both tumor cells and stromal cells play a role in metastasis of cutaneous SCC, and increased MMPs in stromal cells might be induced by basigin/CD147 expressed in tumor cells.
Chen
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Department of Dermatalogy, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Kanekura
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Zhang
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D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D030161:Avian Proteins; D051926:Basigin; D001798:Blood Proteins; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000556:secondary*; D005500:Follow-Up Studies; D006801:Humans; D008198:Lymph Nodes / Q000201:enzymology*; D008207:Lymphatic Metastasis; D020782:Matrix Metalloproteinases / Q000096:biosynthesis*; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D012878:Skin Neoplasms / Q000201:enzymology*
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D030161:Avian Proteins; C497886:BSG protein, human; D001798:Blood Proteins; C102624:Bsg protein, Gallus gallus; C079432:Bsg protein, rat; D008562:Membrane Glycoproteins; D051926:Basigin; D020782:Matrix Metalloproteinases