[An experimental study on the relationship between early postmortem intervals and DNA content of spleen cells in rats by computerized image analysis].
15 rats were used in this experiment. After the rats were executed, spleen cells were collected and smeared in every hour within 24 hours, then fixed with formalin solution and stained with Feulgen's method. Mean and integral optical density and abnormal index of cell DNA were measured with a auto-TV-image system, and the data were statistically analyzed. Results showed that the DNA degeneration rate of rat spleen cells had a linear relationship with early postmortem intervals.
Liu
L
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Department of Forensic Pathology, TongJi Medical University, Wuhan 340030, P. R. China.
D000818:Animals; D003592:Cytophotometry / Q000379:methods; D004247:DNA / Q000032:analysis*; D003643:Death; D007091:Image Processing, Computer-Assisted / Q000379:methods; D051381:Rats; D013154:Spleen / Q000737:chemistry* / Q000166:cytology; D013997:Time Factors
D004247:DNA