[Study on the physicochemical properties of Campylobacter jejuni enterotoxin].
Precipitate of Campylobacter jejuni cytotonic enterotoxin(CE) performed in an 80% saturated solution of ammonium sulfateit indicated that there were some little molecular proteins except the 68 kD main band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE), whereas the eluate from GM1 ganglioside affinity column chromatography exhibited only one 68 kD band on SDS-PAGE. The results suggest that CE mainly be consisted of 68 kD protein. The toxin is heat-labile, pH dependent and resistant to trypsin, It could be completely inactivated by heating at either 56 degrees C and 60 degrees C for 30 min or 100 degrees C for 15 min. The activity was maximum at pH 6.0 and was completely inactivate at pH 3.0 and pH 9.0, and rapidly reduced after storage over 3 d at 4 degrees C. The anti-LT serum could completely inhibited the activity of CE.
Wu
R
R
Department of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070.
Si
H
H
D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D016466:CHO Cells; D002168:Campylobacter fetus / Q000378:metabolism*; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D051381:Rats
D001427:Bacterial Toxins; D004768:Enterotoxins