title: Semi-micro high-performance liquid chromatographic analysis of tiropramide in human plasma using column-switching.
abstract: A rapid and sensitive column-switching semi-micro high-performance liquid chromatography method was developed for the direct analysis of tiropramide in human plasma. The plasma sample (100 microl) was directly injected onto Capcell Pak MF Ph-1 precolumn where deproteinization and analyte fractionation occurred. Tiropramide was then eluted into an enrichment column (Capcell Pak UG C(18)) using acetonitrile-potassium phosphate (pH 7.0, 50 mM) (12:88, v/v) and was analyzed on a semi-micro C(18) analytical column using acetonitrile-potassium phosphate (pH 7.0, 10 mM) (50:50, v/v). The method showed excellent sensitivity (limit of quantification 5 ng/ml), and good precision (C.V.<or=5.9%), and speed (total analysis time 20 min). The calibration curve was linear in the concentration range 5-200 ng/ml (coefficient of determination equal to 0.998). This method was successfully applied to the pharmacokinetic study of tiropramide in volunteers.
authors.affiliation: College of Pharmacy and Medicinal Resources Research Center, Wonkwang University, Shinyongdong, Iksan 570-749, South Korea.
authors.forename: Soo Kyoung
authors.initials: SK
authors.lastname: Baek
authors.forename: Seung Seok
authors.initials: SS
authors.lastname: Lee
authors.forename: Eun Jeon
authors.initials: EJ
authors.lastname: Park
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Sohn
authors.forename: Hye Suk
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D006801:Humans; D008297:Male; D010276:Parasympatholytics / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D013607:Tablets; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D010276:Parasympatholytics; D013607:Tablets; D014443:Tyrosine; C031173:tiropramide