[Studies on application for classification and identification in Mycobacterium by analysis of PCR amplification of 16S-23S ribosomal DNA spacer sequences].
The 16S-23S ribosomal DNA spacer sequence of mycobacterium were amplified by PCR. The products were visualized by PAGE, and evaluate the possibility for classification and identification of mycobacterium at gene level. The sensitivity of PCR in annealing temperature 45 degrees C was 500 fg/microL, whereas 50 degrees C was 5 pg/microL. The results showed that: the amplified bands ranging from 300 to 600 bp, most of rapid-growing Mycobacterium and Nonmycobacterium tested have more bands and the bands molecular weights were larger than slow-growing Mycobacterium. The relativity of mycobacterium < 70%, most of them < 50%. This experimental method might be rapid and effective for differentiation of Mycobacterium at species level.
Zhang
L
L
Tuberculosis Research Laboratory, 309 Hospital of PLA, Beijing 100091.
Zhuang
Y
Y
He
X
X
Zhang
X
X
Li
G
G
D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S