Full-length dystrophin cDNA transfer into skeletal muscle of adult mdx mice by electroporation.
We showed that a LacZ expression plasmid (pCAG-lacZ) injection followed by electroporation increased the expression of the LacZ gene in the skeletal muscles of adult mdx mice up to ninefold higher as compared with simple intramuscular DNA injection. When full-length mouse dystrophin plasmid (pCAG-dys) and pCAG-lacZ were co-transfected by electroporation, 56% of dystrophin-positive fibers were stained for beta-galactosidase activity suggesting most of these myofibers are not revertants but transfected ones. Our data indicate that electroporation in vivo could introduce large full-length dystrophin cDNA into skeletal muscle of adult mdx mice.
Murakami
Tatsufumi
T
Division of Neurology, Department of Internal Medicine, Kawasaki Medical School, 577 Matsushima, Kurashiki City, Okayama 701-0192, Japan. tatsum@med.kawasaki-m.ac.jp
Nishi
Toru
T
Kimura
En
E
Goto
Tomoaki
T
Maeda
Yasushi
Y
Ushio
Yukitaka
Y
Uchino
Makoto
M
Sunada
Yoshihide
Y
D000367:Age Factors; D000818:Animals; D018076:DNA, Complementary / Q000493:pharmacokinetics; D016189:Dystrophin / Q000235:genetics*; D018274:Electroporation / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods*; D007763:Lac Operon; D051379:Mice; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000378:metabolism*; D009137:Muscular Dystrophy, Animal / Q000628:therapy*; D020388:Muscular Dystrophy, Duchenne / Q000628:therapy; D010957:Plasmids / Q000493:pharmacokinetics
D018076:DNA, Complementary; D016189:Dystrophin