SC1/hevin. An extracellular calcium-modulated protein that binds collagen I.
SC1, a member of the BM-40 family of extracellular matrix proteins, was recombinantly expressed in a eukaryotic expression system. The full-length protein as well as truncated versions were purified to homogeneity under non-denaturing conditions. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry of full-length SC1 revealed a mass of 87.8 kDa of which 16.8 kDa is contributed by posttranslational modifications. In electron microscopy, after negative staining, SC1 was revealed as a globule attached to a thread-like structure. A calcium dependence of the SC1 conformation could be demonstrated by fluorescence spectroscopy. In the extracellular matrix of cultured osteosarcoma cells SC1 was found associated with collagen I-containing fibrils, and binding of SC1 to reconstituted collagen I fibrils could be demonstrated by immunogold labeling and electron microscopy. SC1 showed a broad expression in a variety of tissues.
Hambrock
Harald O
HO
Center for Biochemistry, Medical Faculty, University of Cologne, D-50931 Cologne, Germany.
Nitsche
D Patric
DP
Hansen
Uwe
U
Bruckner
Peter
P
Paulsson
Mats
M
Maurer
Patrik
P
Hartmann
Ursula
U
D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002942:Circular Dichroism; D024042:Collagen Type I / Q000378:metabolism*; D017931:DNA Primers; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D051379:Mice; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014407:Tumor Cells, Cultured
D002135:Calcium-Binding Proteins; D024042:Collagen Type I; D017931:DNA Primers; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D011994:Recombinant Proteins; C503127:Sparcl1 protein, mouse; D002118:Calcium