[Determination of the point of gene mutation in two types of tissue from the aminoglycoside antibiotics induced deaf patients].
OBJECTIVE
To elucidate the gene mutation presents in hair follicle cells, just as blood cells, in the aminoglycoside antibiotics induced deaf patients(AAID).


METHODS
Mitochondrial DNA of blood cells and/or hair follicle cells from 8 members of 3 aminoglycoside antibiotics induced deafness families were analysed using PCR-SSCP and Alw 26 I digestian.


RESULTS
Our result showed that a gene mutation at nucleotide 1555 in 12 S rRNA of mitochondrial DNA appeared in blood cells and/or hair follicle cells of 7 subjects among those 8 members. Only a father with normal hearing did't exhibit such a mutation.


CONCLUSIONS
This indicated that hairs could be used as a sample instead of blood to perform gene examination for AAID.
Xu
L
L
Department of Otorhinolaryngology, Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310009.
Chen
W
W
Xing
C
C
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D004272:DNA, Mitochondrial / Q000235:genetics*; D003638:Deafness / Q000139:chemically induced / Q000235:genetics*; D005260:Female; D006198:Hair Cells, Auditory / Q000737:chemistry; D018859:Hair Follicle / Q000737:chemistry; D006801:Humans; D008297:Male; D010375:Pedigree; D017354:Point Mutation
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004272:DNA, Mitochondrial