[Study on the separation of proteins in human serum by high performance capillary zone electrophoresis].
A high performance capillary zone electrophoretic method has been developed for the separation of proteins in human serum. Sera were diluted with a buffer (0.05 mol/L boric acid, pH 8.80), and then introduced hydrodynamically into a 37 cm (32 cm to detector) x 50 microns i.d. fused-silica capillary, electrophoresed for 12 min using a boric buffer solution (0.10 mol/L, pH 9.35; containing 4 g/L of polyethylene glycol 8000), and detected at the wavelength of 200 nm. The method is simple, rapid and repeatable.
Li
K
K
General Hospital of Nanjing Command, PLA, Nanjing 210002, China.
Yuan
Y S
YS
Zhao
F L
FL
D000510:Alpha-Globulins / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D012709:Serum Albumin / Q000302:isolation & purification*; D005719:gamma-Globulins / Q000032:analysis
D000510:Alpha-Globulins; D012709:Serum Albumin; D005719:gamma-Globulins