[The cloning and functional analysis of Sinorhizobium fredii 042BS regulatory modulation genes].
The total DNA of Sinorhizobium fredii 042BS was digested by EcoRI for Southern blotting with probes of nodD1 and nodD2 from S. fredii USDA257. The 3 kb positive band hybrided with nodD1 probe and 6 kb positive band with nodD2 probe were found, respectively. Partial gene library were constructed using pUC18 as vector, and the clones with the nodD1 and nodD2 genes were obtained. The sequence of nodD1 and nodD2 of 042B showed that they are highly homologous with nodD1 and nodD2 of S. fredii. The fragment with nodD1 was cloned into the vector pBBRIMCS-5 and introduced into R. leguminosarum bv. viciae LPR5054 to study the function of the nodD1. The results showed that nodD1 of 042B can be induced by genistein and luteolin secreted by the seedlings of soybean and alfalfa respectively.
Zhang
Haiyu
H
Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Zhang
Haiyu
H
Li
Xiaohong
X
Wen
Shangkun
S
Yan
Susheng
S
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D012231:Rhizobium / Q000235:genetics*; D017385:Sequence Homology; D020579:Sinorhizobium / Q000145:classification / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C060773:NodD protein, Bacteria; D015534:Trans-Activators