[Selection of beta-glucanase-producing Trichoderma köningii T199 and its fermentation conditions].
A mutant strain T199 producing about 8 times as much beta-glucanase as its parent strain trichoderma köningii T3 was obtained by treatment with ultraviolet light and N-methyl-N-nitro-N-nitrosoguanidine. Fermentation was conducted in 250 mL flask, each containing 30 mL of medium consisted of 5% corn cob powder, 3% wheat bran and 1.4% nitrogen source No. 10 ((NH4)2SO4 10%, Peptone 20%, Yeast extract 15%). The optima culture conditions were as below: initial pH 5.0, 30 degrees C, shaking speed 280 r/min, and cultivation time 5 d. Enzyme activity toward CMC-Na, lichenin, laminarin and barley beta-glucan at pH 5.0 and 60 degrees C for 10 min were 300, 1100, 12 and 1600 IU/mL, respectively. The optima pH and temperature for enzyme action toward barley beta-glucan were pH 5.0 and 70 degrees C, respectively. The enzyme was stable under below 50 degrees C and at pH 4.5-6.5.
Shi
J
J
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Cui
F
F
D002480:Cellulase / Q000096:biosynthesis; D044602:Cellulases / Q000096:biosynthesis; D005285:Fermentation; D043326:Glucan 1,3-beta-Glucosidase / Q000096:biosynthesis*; D006026:Glycoside Hydrolases / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009154:Mutation / Q000187:drug effects / Q000528:radiation effects; D013696:Temperature; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D014466:Ultraviolet Rays
D008769:Methylnitronitrosoguanidine; D044602:Cellulases; D006026:Glycoside Hydrolases; D002480:Cellulase; D043326:Glucan 1,3-beta-Glucosidase; C028020:licheninase