Effect of matrine on cold ischemia and reperfusion injury of sinusoidal endothelial cells in rat orthotopic liver transplantation.
OBJECTIVE
To study the mechanism and prevention of matrine (Mat) on cold ischemia/reperfusion injury of sinusoidal endothelial cells (SEC) in rat orthotopic liver transplantation (OLT).


METHODS
One hundred and twenty-six syngeneic SD rats were randomly divided into four groups (n=18): untreated group, 40 mg/kg treated group, 80 mg/kg treated group, and pseudo-treated group. After 5 h of preservation in Ringer's (LR) solution, orthotopic implantation of the donor liver was performed. At 1, 2, and 4 h after reperfusion of the portal vein, 6 rats were killed in each group to collect the serum and the median lobe of liver for assay.


RESULTS
The level of hylluronic acid (HA) and intercellular adhesion molecule-1 (ICAM-1) decreased significantly in both treated groups at different times post-transplantation, and their pathological changes of SEC were ameliorated, too.


CONCLUSIONS
Matrine can prevent SEC from ischemia and reperfusion injury in rat orthotopic liver transplantation.
Zhu
Xin-Hua
XH
Department of Hepatobiliary Surgery, Drum Tower Hospital, Medical Department of Nanjing University, 210008, China. drzhu@elong.com
Qiu
Yu-Dong
YD
Shi
Ming-Ke
MK
Wu
Bo
B
Zheng
Xiao-Gang
XG
Ding
Yi-Tao
YT
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D003080:Cold Temperature; D042783:Endothelial Cells / Q000378:metabolism; D006820:Hyaluronic Acid / Q000097:blood; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D007511:Ischemia; D008099:Liver / Q000378:metabolism / Q000473:pathology; D016031:Liver Transplantation; D008297:Male; D020011:Protective Agents / Q000494:pharmacology; D011807:Quinolizines; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000093842:Matrines
D000470:Alkaloids; D020011:Protective Agents; D011807:Quinolizines; D018799:Intercellular Adhesion Molecule-1; D006820:Hyaluronic Acid; D000093842:Matrines