[Identification of cry-type genes of 31 Bacillus thuringiensis isolates and analysis of their expression product].
In this study, cry-type gene of 31 isolates of Bt had been identified using PCR-RFLP identification system, and SDS-PAGE analysis and bioassay had been performed. 25 strains containing cry1 type gene express 130-150 kD protein and 16 of them contain cry1I gene, which codes 80 kD protein and is larvicidal to both lepidopteran and coleopteran species. 15 strains contain both cry1 and cry2 type gene; 10 strains contain unknown cry-type gene; 6 strains don't contain seven identified cry-type gene. Result of bioassay shows that the strains containing both cry1 and cry2 gene have high toxicity to lepidopteran order, 7 of them are strongly insecticidal to Pristiphora beijingensis and Lymantria dispar larvae; the strains containing cry1Ab + 1Ac + 2A or cry1Aa + 1Ac + 2A have high activity to Heliothis armigera; no cry gene, no activity. It is indicated that identification of cry-type gene and its analysis of SDS-PAGE protein can predict insecticidal activity of Bt isolates, and it is no pertinency between cry-type gene and serotype of Bt strain.
Zhang
J
J
Inst. of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094.
Song
F
F
Zuo
Y
Y
Dai
L
L
Huang
D
D
D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000633:toxicity; D001427:Bacterial Toxins / Q000235:genetics* / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000235:genetics* / Q000633:toxicity; D015870:Gene Expression; D005838:Genotype; D006460:Hemolysin Proteins; D006927:Hymenoptera / Q000187:drug effects; D007306:Insecticides / Q000633:toxicity; D007814:Larva; D007915:Lepidoptera / Q000187:drug effects; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis