title: Novel method for the immobilization of nucleotides.
abstract: [reaction: see text] A novel method for the immobilization of nucleotides has been developed. The strategy employs a highly reactive pyrrolidinium phosphoramidate zwitterion intermediate that undergoes nucleophilic attack by long-chain alkylamine-controlled pore glass (LCAA-CPG) to generate an immobilized nucleotide. Quantification of nucleotide loading was accomplished by acidic hydrolysis of the P-N bond and subsequent HPLC analysis of TMP in the presence of an internal standard. Typical nucleotide loadings of 51-59 micromol/g of support were observed.
authors.affiliation: Department of Medicinal Chemistry and Molecular Pharmacology and Cancer Center, Purdue University, West Lafayette, Indiana 47907, USA. caren_meyers@hms.harvard.edu
authors.forename: Caren L
authors.initials: CL
authors.lastname: Freel Meyers
authors.forename: Richard F
authors.initials: RF
authors.lastname: Borch
mesh_terms: D000577:Amides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D009711:Nucleotides / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010756:Phosphoric Acids / Q000737:chemistry
chemical_list: D000577:Amides; D009711:Nucleotides; D010756:Phosphoric Acids; C011067:phosphoramidic acid