Krapfen/dMyd88 is required for the establishment of dorsoventral pattern in the Drosophila embryo.
In Drosophila, the dorsoventral axis is set up by the action of the dorsal group of genes and cactus, which have been ordered genetically in a linear pathway. We have identified and characterised krapfen (kra) as a new member of the dorsal-group genes. kra encodes for the Drosophila homologue of MyD88, an adapter protein operating in the mammalian IL-1 pathway. Epistasis experiments reveal that kra acts between the receptor Toll and the cytoplasmic factor Tube. We show that there is a direct interaction between Kra and Tube presumably mediated by the death domains present in both proteins. Tube in turn interacts with its downstream effector Pelle through death domain association. We therefore suggest that upon Toll activation, Kra associates with Pelle and Tube, in an heterotrimeric complex.
Charatsi
Iphigenie
I
Abteilung Genetik, Max-Planck-Institut für Entwicklungsbiologie, Spemannstrasse 35, 72076 Tubingen, Germany.
Luschnig
Stefan
S
Bartoszewski
Slawomir
S
Nüsslein-Volhard
Christiane
C
Moussian
Bernard
B
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism*; D019521:Body Patterning / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D053594:Myeloid Differentiation Factor 88; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D051193:Toll-Like Receptors
D048868:Adaptor Proteins, Signal Transducing; D000943:Antigens, Differentiation; D029721:Drosophila Proteins; C499312:Myd88 protein, Drosophila; D053594:Myeloid Differentiation Factor 88; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; C068721:Tl protein, Drosophila; D051193:Toll-Like Receptors; C084727:tub protein, Drosophila; C079896:pll protein, Drosophila; D017346:Protein Serine-Threonine Kinases