[Studies on microinjection of mouse spermatozoa into rabbit oocytes].
The methods of intracytoplasmic sperm injection (ICSI) and subzonal injection (SUZI) were used to study heterologous fertilization and embryonic development between the mouse and the rabbit. Results were as follows: 1. The mouse sperm nuclei decondensed and formed pronuclei following microinjection into cytoplasm and perivitelline space (PVS) of rabbit oocytes; 2. The hybrid embryos developed to the stage of 8-cell when cultured in vitro; 3. The karyotype analysis showed a normal complement of rabbit oocyte and mouse sperm chromosomes in the 4-cell hybrid embryos; 4. The ultrastructure of 4-cell hybrid embryos was similar to that of normal 4-cell rabbit embryos; 5. The fertilization rate (32.4%) and cleavage rate (22.2%) when 5-10 mouse spermatozoa were injected were higher than those of injection of a single spermatozoon into PVS of the rabbit oocyte, but the difference was not significant (P > 0.05). The fertilization rate (42.3%) and cleavage rate (30.8%) in rabbit oocytes in vitro matured for 11-12 h were higher than those in the oocytes which were in vitro matured for 24-25 h following microinjection of 1-2 mouse spermatozoa into PVS, but the difference was not significant (P > 0.05).
Li
Z Y
ZY
Dept. of Biotechnology, Northeast Agricultural University, Harbin 150030, China.
Tan
J H
JH
Zhou
Q
Q
Sun
X S
XS
Li
G P
GP
Liu
Z H
ZH
D000818:Animals; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000648:ultrastructure*; D005260:Female; D008297:Male; D051379:Mice; D008845:Microinjections; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D011817:Rabbits; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000502:physiology*