title: G protein-dependent CCR5 signaling is not required for efficient infection of primary T lymphocytes and macrophages by R5 human immunodeficiency virus type 1 isolates.
abstract: The requirement of human immunodeficiency virus (HIV)-induced CCR5 activation for infection by R5 HIV type 1 (HIV-1) strains remains controversial. Ectopic CCR5 expression in CD4(+)-transformed cells or pharmacological inhibition of G(alpha)i proteins coupled to CCR5 left unsolved whether CCR5-dependent cell activation is necessary for the HIV life cycle. In this study, we investigated the role played by HIV-induced CCR5-dependent cell signaling during infection of primary CD4-expressing leukocytes. Using lentiviral vectors, we restored CCR5 expression in T lymphocytes and macrophages from individuals carrying the homozygous 32-bp deletion of the CCR5 gene (ccr5 Delta32/Delta32). Expression of wild-type (wt) CCR5 in ccr5 Delta32/Delta32 cells permitted infection by R5 HIV isolates. We assessed the capacity of a CCR5 derivative carrying a mutated DRY motif (CCR5-R126N) in the second intracellular loop to work as an HIV-1 coreceptor. The R126N mutation is known to disable G protein coupling and agonist-induced signal transduction through CCR5 and other G protein-coupled receptors. Despite its inability to promote either intracellular calcium mobilization or cell chemotaxis, the inactive CCR5-R126N mutant provided full coreceptor function to several R5 HIV-1 isolates in primary cells as efficiently as wt CCR5. We conclude that in a primary, CCR5-reconstituted CD4(+) cell environment, G protein signaling is dispensable for R5 HIV-1 isolates to actively infect primary CD4(+) T lymphocytes or macrophages.
authors.affiliation: Unité d'Immunologie Virale, Institut Pasteur, F-75724 Paris Cedex 15, France. aamara@pasteur.fr
authors.forename: Ali
authors.initials: A
authors.lastname: Amara
authors.forename: Aurore
authors.initials: A
authors.lastname: Vidy
authors.forename: Genevieve
authors.initials: G
authors.lastname: Boulla
authors.forename: Karine
authors.initials: K
authors.lastname: Mollier
authors.forename: Javier
authors.initials: J
authors.lastname: Garcia-Perez
authors.forename: Jose
authors.initials: J
authors.lastname: Alcamí
authors.forename: Cedric
authors.initials: C
authors.lastname: Blanpain
authors.forename: Marc
authors.initials: M
authors.lastname: Parmentier
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Virelizier
authors.forename: Pierre
authors.initials: P
authors.lastname: Charneau
authors.forename: Fernando
authors.initials: F
authors.lastname: Arenzana-Seisdedos
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D002460:Cell Line; D002478:Cells, Cultured; D019204:GTP-Binding Proteins / Q000378:metabolism*; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008264:Macrophages / Q000821:virology*; D019713:Receptors, CCR5 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014779:Virus Replication
chemical_list: D019713:Receptors, CCR5; D019204:GTP-Binding Proteins