Determination of trace amounts of bisphenol A in urine by negative-ion chemical-ionization-gas chromatography/mass spectrometry.
We improved an analytical method for determining trace amounts of bisphenol A (BPA) in urine. BPA was subjected to enzymolysis and then to solid phase extraction with a C18 cartridge. The extract was eluted with methanol, and the eluate was concentrated under a nitrogen stream, and then pentafluorobenzylized in an alkali solution. The obtained pentafluorobenzylized compound was purifed using a florisil cartridge, followed by a determination using NCI-GC/MS. This method exhibited an excellent selectivity and reproducibility with a determination limit of 0.1 ng/ml.
Tsukioka
Tadashi
T
Nagano Research Institute for Health and Pollution, 1978 Komemura-Amori, Nagano 380-0944, Japan.
Brock
John
J
Graiser
Sam
S
Nguyen
Johnny
J
Nakazawa
Hiroyuki
H
Makino
Tsunehisa
T
D001559:Benzhydryl Compounds; D002138:Calibration; D005464:Fluorobenzenes / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006868:Hydrolysis; D010636:Phenols / Q000652:urine*; D015203:Reproducibility of Results; D005466:Fluorocarbons; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D005464:Fluorobenzenes; D010636:Phenols; C025063:pentafluorobenzyl bromide; D005466:Fluorocarbons; C006780:bisphenol A; D000098826:Bisphenol A Compounds