[The application of minisatellite MS31A MVR-PCR digital coding technique in forensic science].
Using isotope incorporate amplification technique of special 31A, 31A-A, 31A-G primers and alpha-32PdCTP, the minisatellite MS31A (located at D7S21 loci) was studied. A rapid, simple, and accurate MVR-PCR technique was successfully established. The technique can be applied in individual identification minutes amples, such as blood stains, semen stains contaminated by vaginal fluid, hair and bones. The sensitivity analysis revealed that this technique could detect 1 ng genoma DNA. It is also discribed about the application of the method in 40 criminal cases of rape and murder.
Hou
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Forensic Science Institute of Liaoning Province, Shenyang 110032, P. R. China.
D001801:Blood Stains; D001842:Bone and Bones / Q000737:chemistry; D004276:DNA, Satellite / Q000235:genetics*; D005554:Forensic Medicine; D006197:Hair / Q000737:chemistry; D006801:Humans; D008297:Male; D018598:Minisatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013094:Spermatozoa / Q000737:chemistry
D004276:DNA, Satellite