Proliferative ability and alkaline phosphatase activity with in vivo cellular aging in human pulp cells.
Little is known about the effect of aging on characteristic functions of pulp cells. When damaged pulp is recovered and mineralized tissue is formed to protect remaining pulp tissue, the general responses of pulp tissue after adequate stimuli (pulp cell proliferation and activation of alkaline phosphatase [ALPase]) are thought to be essential. In this study, we compared proliferative ability and ALPase activity between cultures of human pulp (HP) cells obtained from young and aged donors. The in vitro proliferative lifespan of HP cells from young donors was longer than HP cells from aged donors. Growth rates and ALPase activity of HP cells decreased with increasing donor age. These findings suggest that impaired repair of pulp and dentin in aged patients is partly due to a decrease in the proliferative ability and ALPase activity in aged pulp cells.
Shiba
Hideki
H
Department of Periodontology and Endodontology, Hiroshima University, Faculty of Dentistry, Japan.
Nakanishi
Keiji
K
Rashid
Fahmida
F
Mizuno
Noriyoshi
N
Hino
Takamune
T
Ogawa
Tetsuji
T
Kurihara
Hidemi
H
D000293:Adolescent; D000368:Aged; D000469:Alkaline Phosphatase / Q000378:metabolism; D002455:Cell Division; D016922:Cellular Senescence / Q000502:physiology*; D002648:Child; D003782:Dental Pulp / Q000166:cytology* / Q000201:enzymology; D006801:Humans; D008875:Middle Aged
D000469:Alkaline Phosphatase