Transcription factors GATA-4 and GATA-6 during mouse and human adrenocortical development.
Our earlier work implicates transcription factors GATA-4 and GATA-6 in the murine adrenal. We have now studied their expression during mouse and human adrenal development in detail. GATA-4 and GATA-6 mRNAs are readily detectable from embryonic day 15 in mouse and gestational week 19 in human adrenal cortex. In postnatal adrenal, GATA-4 expression is down-regulated, whereas GATA-6 mRNA and protein continue to be abundantly present. In a human adrenocortical cell line NCI-H295R, GATA-6 mRNA is up-regulated by cAMP. This cell line does not express GATA-4. Our findings suggest that GATA-6 expression is hormonally controlled, and required throughout adrenal development from fetal to adult age. GATA-4, on the other hand, may serve a role in fetal adrenal gene regulation.
Kiiveri
S
S
Children's Hospital, P.O. Box 63, 00014, University of Helsinki, Finland.
Liu
J
J
Westerholm-Ormio
M
M
Narita
N
N
Wilson
D B
DB
Voutilainen
R
R
Heikinheimo
M
M
D000302:Adrenal Cortex / Q000196:embryology*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism; D005314:Embryonic and Fetal Development; D050991:GATA4 Transcription Factor; D050993:GATA6 Transcription Factor; D006801:Humans; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D004268:DNA-Binding Proteins; D050991:GATA4 Transcription Factor; D050993:GATA6 Transcription Factor; C494759:GATA6 protein, human; C494760:Gata6 protein, mouse; D012333:RNA, Messenger; D014157:Transcription Factors; D003994:Bucladesine