[The foundation of the injury model on cultured human fetal lung fibroblasts and its immunohistochemistry research].
Cultured human fetal lung Fbs was first used to study the relationship between wound age and the change of cFn synthesized by Fbs in Vitro. A sterilized injector was applied to scrap the cultured confluent Fbs and a "wound" was obtained. Fbs around the wound was seen to change from a stationary stage to an active stage. Using the method of immunochemistry and image analysis system (IAS), cFn synthesized by Fbs around the wound was observed at once, 30 min, 1 h, 3 h and 6 h after injury. It was found that cFn began to change 1 h after injury and increased continually within 6 h. It suggests that the change of cFn synthesized by Fbs is time-dependent after injury and this approach breaks a new path in the study on estimation of wound age.
Shen
Y W
YW
Department of Forensic Medicine, Shanghai Medical University, Shanghai 200032, P. R. China.
Zhao
Z Q
ZQ
Chen
Y J
YJ
Yan
Z K
ZK
Gu
Y J
YJ
Wu
W Q
WQ
Liao
Y P
YP
D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D005353:Fibronectins / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D013997:Time Factors; D014945:Wound Healing
D005353:Fibronectins