title: [Detecting microorganisms in soil by PCR and molecular hybridization].
abstract: PCR and molecular hybridization provide new methods to detect microorganisms in environment. A simple, sensitive protocol was developed to detect Bacillus thuringiensis HD-1 in soil by PCR and Southern Blotting in this study. Methods of extracting DNA from soil and purification were compared. By a modified total DNA extraction from soil and purification, followed by PCR and Southern Blotting, strain HD-1 released into soil can be detected with a sensitivity of 2.2 x 10(2) cells/g soil. By using other specific primers, this detection protocol can be widely used in the research of ecology and risk assessment of genetically engineered microorganisms released into environment.
authors.affiliation: Environmental Biology and Ecology Department, College of Life Sciences, Peking University, Beijing 100871.
authors.forename: Z
authors.initials: Z
authors.lastname: Ding
authors.forename: C
authors.initials: C
authors.lastname: Xu
mesh_terms: D001413:Bacillus thuringiensis / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial