Interaction between various resistance modifiers and apoptosis inducer 12H-benzo[alpha]phenothiazine.
The effect of some resistance modifiers on apoptosis induction by a benzo[alpha]phenothiazine derivative was studied on the L5178Y mouse lymphoma cells (parent) and its multidrug resistant (MDR) subline. For evaluation of apoptosis the cells were stained with FITC-labelled annexin V and propidium iodide and the results were analysed by flow cytometry. 12H-benzo[alpha]phenothiazine [M627] induced apoptosis both in the parent cells and in the MDR cells. The apoptosis induction by [M627] was not affected significantly by post- or pre-treatment with resistance modifiers, while in the cells treated by (+/-)-verapamil before and after apoptosis induction with [M627], the apoptosis was somewhat higher. The resistance modifier compounds alone also induced apoptosis and it was slightly higher in the parent cells than its MDR1/A gene-transformed subline.
Mucsi
Ilona
I
Department of Medical Microbiology, Faculty of General Medicine, University of Szeged, Hungary.
Varga
Andreas
A
Kawase
Masami
M
Motohashi
Noboru
N
Molnar
Joseph
J
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002147:Calmodulin / Q000037:antagonists & inhibitors; D004347:Drug Interactions; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D006845:Hydrocarbons, Fluorinated / Q000494:pharmacology; D016399:Lymphoma, T-Cell / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D009853:Omeprazole / Q000494:pharmacology; D010080:Oxazoles / Q000494:pharmacology; D010640:Phenothiazines / Q000494:pharmacology*; D011398:Promethazine / Q000494:pharmacology; D054328:Proton Pump Inhibitors; D014407:Tumor Cells, Cultured; D014700:Verapamil / Q000494:pharmacology
D002147:Calmodulin; D006845:Hydrocarbons, Fluorinated; D010080:Oxazoles; D010640:Phenothiazines; D054328:Proton Pump Inhibitors; C107575:benzophenothiazine; D014700:Verapamil; D011398:Promethazine; D009853:Omeprazole