title: Removal of PCR inhibitors from soil DNA by chemical flocculation.
abstract: Extracting high-purity DNA directly from soil has become essential for the study of microorganisms in environmental samples. However, many soils contain compounds that inhibit enzymes involved in manipulating DNA. In this study, chemical flocculation using multivalent cations was investigated as a potential method for eliminating soil-based inhibitors during the extraction process. The addition of AlNH(4)(SO(4))(2) during extraction significantly reduced the co-purification of PCR inhibitors with minimal loss of DNA yield.
authors.affiliation: Ciphergen Biosystems, Incorporated, 6611 Dumbarton Circle, Fremont, CA 94555, USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Braid
authors.forename: Laura M
authors.initials: LM
authors.lastname: Daniels
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Kitts
mesh_terms: D000534:Alum Compounds / Q000378:metabolism; D002122:Calcium Chloride / Q000378:metabolism; D002412:Cations / Q000819:agonists; D002712:Chlorides; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005290:Ferric Compounds / Q000378:metabolism; D005427:Flocculation; D015636:Magnesium Chloride / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D012988:Soil Microbiology
chemical_list: D000534:Alum Compounds; D002412:Cations; D002712:Chlorides; D004269:DNA, Bacterial; D005290:Ferric Compounds; D015636:Magnesium Chloride; C059726:aluminum ammonium sulfate; D002122:Calcium Chloride; C024555:ferric chloride