title: Effect of high-fat diet on sarcoplasmic reticulum Ca(++)-ATPase activity in different types of rat skeletal muscle.
abstract: The aim of the present study was to examine the effect of a high-fat diet on the Ca(2+)-ATPase activity in the sarcoplasmic reticulum of the flexor digitorum longus (fast-twitch, glycolytic muscle) and the soleus (slow-twitch, oxidative muscle). The enzyme activity in the extensor digitorum longus was several-fold higher than in the soleus. The high-fat diet increased the enzyme activity by 22% (p < 0.05) in the soleus but it had no effect in the flexor digitorum longus. It is concluded that a high-fat diet increases the activity of only one isoform of the enzyme, namely the one which is present in the slow-twitch oxidative fibers.
authors.affiliation: Institute of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense University, Denmark.
authors.forename: Lisbet
authors.initials: L
authors.lastname: Aagaard
authors.forename: Artur
authors.initials: A
authors.lastname: Nawrocki
authors.forename: Klavs
authors.initials: K
authors.lastname: Madsen
mesh_terms: D000818:Animals; D000252:Calcium-Transporting ATPases / Q000032:analysis / Q000378:metabolism*; D046508:Culture Techniques; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004789:Enzyme Activation; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000503:physiopathology; D011336:Probability; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D012680:Sensitivity and Specificity
chemical_list: D004041:Dietary Fats; D000252:Calcium-Transporting ATPases