[Detection of coxsackie virus B3 gene in myocardium and spleen samples in myocarditis with sudden death].
OBJECTIVE
To detect the Coxsackie virus B3(CVB3) gene in myocardium and spleen tissues in viral myocarditis(VMC) with sudden death and to explore the diagnostic method for VMC by means of seeking pathogene.


METHODS
By in situ RT-PCR, the detection of CVB3 gene in myocardium and spleen sections were performed in sudden death group caused by VMC and non-cardiac death group.


RESULTS
In VMC group, CVB3 gene-positive signals were seen in myocardium sections(3 out of total 8 cases, No. 1, 4, 7 cases) and spleen sections(4 out of total 8 cases, No. 2, 4, 6, 7 cases). In non-cardiac death group, no positive signals were detected in both myocardium and spleen tissues.


CONCLUSIONS
Positive detection of CVB3 gene in both myocardium and spleen maybe an important character of VMC and can improve the detecting pathogene in diagnosing VMC.
Chen
Y C
YC
Department of Forensic Pathology, Sun Yat-sen University of Medical Sciences, Guangzhou 510080.
Cheng
J D
JD
Ma
H H
HH
Hu
B J
BJ
Yao
C L
CL
D003645:Death, Sudden; D029822:Enterovirus B, Human / Q000235:genetics*; D006321:Heart / Q000821:virology*; D006801:Humans; D009205:Myocarditis / Q000175:diagnosis / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000821:virology*