Subcellular identification of angiotensin I/II- and angiotensin II (AT1)-receptor-immunoreactivity in the central nervous system of rats.
To gain insight into generating and transport mechanisms of angiotensin (ANG) in the brain the study was focused on the subcellular localization of ANG II and its AT(1)-receptors in the hypothalamus of rats. The present paper demonstrates ANG II- and AT(1)-receptor-immunolabelling at brain parenchyma vessels and at glial and neuronal structures in the perivascular region. Further, ANG II- and AT(1)-receptor-immunoreactivity is shown at plasma membranes and intracellular structures in the ependyma of the third ventricle. Based upon a conventional horseradish peroxidase technique, combined with the classical substrate 3,3'-diaminobenzidine, a procedure is introduced that will be useful with a variety of antibodies used on glutar- and paraformaldehyde-fixed brain tissue. This technique enables a fast correlation between light and electron microscopical results and might also provide an attractive alternative to colloidal gold-labelling and silver-intensification techniques.
Thomas
Martin Alexander
MA
Institute of Pharmacology and Toxicology, Ruprecht-Karls University of Heidelberg, Heidelberg, Germany. mthomas@stud.uni-frankfurt.de
Fleissner
Gerta
G
Hauptfleisch
Stefan
S
Lemmer
Björn
B
D000803:Angiotensin I / Q000032:analysis*; D000804:Angiotensin II / Q000032:analysis*; D000818:Animals; D016477:Artifacts; D001369:Axons / Q000648:ultrastructure; D001921:Brain / Q000166:cytology* / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D004727:Endothelium / Q000166:cytology / Q000648:ultrastructure; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D009411:Nerve Endings / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats; D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin / Q000032:analysis / Q000502:physiology*
D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin; D000804:Angiotensin II; D000803:Angiotensin I