[Protective effect of tanshinone on injured cultured PC12 cells in vitro].
OBJECTIVE
To investigate the protective effects of tanshinone on ischemia-like injury models.


METHODS
Six ischemia models including hypoxia, hypoglucose, oxidant injury, calcium overload, nitric oxide neurotoxicity and glutamic acid injury were used to assay the anti-ischemic roles of tanshinone in cultured PC12 cells, by using morphological examination, MTT staining and LDH measurement.


RESULTS
It was found that 3. 125-200 microliters.ml-1 tanshinone possessed obvious protective effects on PC12 cells from six ischemia-like injury models. Tanshinone could increase the number of life cells and decrease LDH activity significantly, particularly in hypoxia and caffeine injured model.


CONCLUSIONS
Tanshinone protected PC12 cells from all injury models effectively in vitro. Its action may mainly focus on inhibiting development of the primary period of ischemia injury and calcium overloading injury.
He
L N
LN
Biological & Medicinal Central Laboratory of Anhui, Hefei 230061, Anhui, China.
Yang
J
J
Jiang
Y
Y
Wang
J
J
Liu
C
C
He
S B
SB
D045784:Abietanes; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D010616:Phenanthrenes / Q000494:pharmacology*; D051381:Rats
D045784:Abietanes; D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D010616:Phenanthrenes; C021751:tanshinone; D007770:L-Lactate Dehydrogenase