Assessment of screening methods for the identification of genetically modified potatoes in raw materials and finished products.
Qualitative polymerase chain reaction methods for the detection of genetically modified potatoes have been investigated that can be used for screening purposes and identification of insect-resistant and virus-resistant potatoes in food. The presence of the nos terminator from Agrobacterium tumefaciens and the antibiotic marker gene nptII (neomycin-phosphotransferase II) was demonstrated in three commercialized Bt-potato lines (Monsanto Co., St. Louis, MO, USA) and one noncommercial GM-potato product (high amylopectin starch, AVEBE, Veendam, The Netherlands) and allows for general screening in foods. For further identification, specific primers for the FMV promoter derived from the figwort mosaic virus, the CryIIIA gene (delta-endotoxin from Bacillus thuringiensis subsp. tenebrionis), potato leafroll virus replicase gene, and the potato virus Y coat protein gene, were designed. The methods described were successfully applied to processed potato raw materials (dehydrated potato powders and flakes), starch samples, and finished products.
Jaccaud
Etienne
E
Department of Quality and Safety Assurance, Nestlé Research Center, Nestec Ltd., Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland.
Höhne
Michaela
M
Meyer
Rolf
R
D016960:Agrobacterium tumefaciens / Q000235:genetics; D000594:Amino Acid Oxidoreductases / Q000235:genetics; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000235:genetics; D017796:Caulimovirus / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis; D030841:Food, Genetically Modified; D007313:Insecta; D019868:Kanamycin Kinase / Q000235:genetics; D009029:Mosaic Viruses / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011198:Solanum tuberosum / Q000235:genetics*; D014780:Viruses
D018744:DNA, Plant; D004279:DNA, Viral; D010940:Plant Proteins; C043222:patatin protein, Solanum tuberosum; D000594:Amino Acid Oxidoreductases; C021883:nopaline synthase; D019868:Kanamycin Kinase; D002265:Carboxylic Ester Hydrolases