title: A potential virulence gene, hylEfm, predominates in Enterococcus faecium of clinical origin.
abstract: An open reading frame (hyl(Efm)) with homologies to previously described hyaluronidase genes has been identified in nonstool isolates of Enterococcus faecium. E. faecium isolates (n=577) from diverse sources were screened for the presence of hyl(Efm) and esp(Efm), a putative virulence gene associated with epidemic E. faecium strains. The presence of esp(Efm) was roughly twice that of hyl(Efm), but both were found primarily in vancomycin-resistant E. faecium isolates in nonstool cultures obtained from patients hospitalized in the United States. These data suggest that specific E. faecium strains may be enriched in determinants that make them more likely to cause clinical infections. Differences in the prevalence of these strains may help explain variations in the clinical importance of multiresistant E. faecium across different continents.
authors.affiliation: Research and Medical Services, Louis Stokes Cleveland Veterans Affairs Medical Center and Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA. louis.rice@med.va.gov
authors.forename: Louis B
authors.initials: LB
authors.lastname: Rice
authors.forename: Lenore
authors.initials: L
authors.lastname: Carias
authors.forename: Susan
authors.initials: S
authors.lastname: Rudin
authors.forename: Carl
authors.initials: C
authors.lastname: Vael
authors.forename: Herman
authors.initials: H
authors.lastname: Goossens
authors.forename: Carola
authors.initials: C
authors.lastname: Konstabel
authors.forename: Ingo
authors.initials: I
authors.lastname: Klare
authors.forename: Sreedhar R
authors.initials: SR
authors.lastname: Nallapareddy
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Huang
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Murray
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D003428:Cross Infection / Q000382:microbiology; D016984:Enterococcus faecium / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics; D014640:Vancomycin / Q000494:pharmacology; D020713:Vancomycin Resistance; D014774:Virulence / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D008565:Membrane Proteins; C116707:enterococcal surface protein, esp; D014640:Vancomycin; D006821:Hyaluronoglucosaminidase