[Determination of aconitine in biological samples].
The determination of aconitine in biological samples by using TLC, HPLC and animal tests is described. TLC is simple, easy to manipulate and highly sensitive. The minimum detection limit was 0.3 microgram, so it can be used as a preliminary screening method. While detecting by HPLC, the characteristic UV-absorption spectrum of aconitine and animal tests were used as the qualitative methods. The characteristic UV absorption wavelengths were (228 +/- 2) nm and (275 +/- 2) nm. The linear range of calibration curve was 2 mg/L-50 mg/L. The coefficient of variation was 0.9996. It is suitable for applications in forensic field.
Liu
Xian-ping
XP
Forensic Medical Examination and Identity Center, Beijing Public Security Bureau, Beijing 100085, China.
Yang
Shi-yun
SY
Pan
Guan-min
GM
D000157:Aconitine / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D005554:Forensic Medicine; D005750:Gastric Juice / Q000737:chemistry*; D006801:Humans; D008099:Liver / Q000737:chemistry; D051379:Mice
D000157:Aconitine