[RAPD method for the identification of intergeneric asymmetric somatic hybrid plants of wheat].
Randomly amplified polymorphic DNA (RAPD) method was used to identify the hybrid nature of three kinds of intergeneric asymmetric somatic hybrid plants of wheat: wheat (Triticum aestivum) + Haynaldia villosa, Wheat + Leymus chinensis and wheat + Agropyron elongatum. It was shown from the electrophoresis profiles that the genome of somatic hybrid plants contained specific section genome of both parents after DNA amplification with arbitrary primers. A specific RAPD product (DNA fragment of 0.77 kbp) of A. elongatum generated with primer OPJ-12 was isolated, purified, labeled and used as a probe. Southern blot from OPJ-12 primer-generated specific section genome of the hybrid (T. aestivum + A. elongatum) hybridized to this probe (0.77 kbp) proved that they are homologous in nature. This paper also discussed the advantage of RAPD method in identification of hybrid plants, especially asymmetric somatic hybrids.
Xia
G M
GM
School of Life Science, Shandong University, Jinan 250100.
Li
Z Y
ZY
Zhou
A F
AF
Chen
H M
HM
D018745:Genome, Plant; D006824:Hybridization, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D014908:Triticum / Q000235:genetics*