The rate of apoptosis in post mitotic neutrophil precursors of normal and neutropenic humans.
Using data on the fraction of post-mitotic neutrophil precursors (CD15+ cells) displaying positive markers for apoptosis in 12 normal humans, and a simple mathematical model, we have estimated the apoptotic rate to be about 0.28/day in this compartment. This implies that the influx of myelocytes into the post-mitotic compartment exceeds twice the granulocyte turnover rate (GTR), and that about 55% of the cells entering this compartment die before being released into the blood. The normal half life of apoptotic post-mitotic neutrophil precursors is calculated to be 10.4 h. Comparable calculations for patients indicate apoptosis rates in the post-mitotic compartment of about 17 times normal for one myelokathexis patient and rates of about 13 times normal for the one cyclical neutropenic patient and two severe congenital neutropenic patients. The estimated half life for apoptotic post-mitotic neutrophil precursors in the myelokathexis patient was about 0.4 h, 1.4 h in the cyclical neutropenia patient, and about 0.6 h in the severe congenital neutropenic patients.
Mackey
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MC
Department of Physiology, Centre for Nonlinear Dynamics, McGill University, 3655 Drummond Street, Room 1124, Montreal, Quebec, Canada H3G 1Y6. mackey@cnd.mcgill.ca
Aprikyan
A A G
AA
Dale
D C
DC
D015703:Antigens, CD / Q000032:analysis; D017209:Apoptosis; D015415:Biomarkers; D002453:Cell Cycle; D002454:Cell Differentiation; D002455:Cell Division; D019070:Cell Lineage; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008954:Models, Biological; D008962:Models, Theoretical; D009503:Neutropenia / Q000097:blood* / Q000151:congenital / Q000473:pathology; D009504:Neutrophils / Q000166:cytology / Q000502:physiology*
D015703:Antigens, CD; D015415:Biomarkers