The canine mast cell activation via CRP.
We report here canine mastocytoma-derived cell (CMMC) activation via two pentraxin, limulus- and human-CRP. Mast cell chemotaxis was measured by Boyden's blindwell chamber. To confirm that the cell migration was chemotactic, "checkerboard" analysis was performed. We used Fura-2 to investigate CRP-mediated cytosolic calcium elevation. To examine whether CRP-induced stimulation is mediated through G-proteins, CMMC were incubated with pertussis toxin (PTx) before use in chemotaxis assay and Ca(2+) mobilization. CMMC migration in response to CRP was both chemokinetic and chemotactic. Limulus-CRP induced a transient Ca(2+)-mobilization dose-dependently. Preincubation of the cells with PTx inhibited CRP chemotaxis and Ca(2+)-mobilization, suggesting that G-proteins of the Gi-class are involved in the chemotaxis. We suggest that CRP may participate in the migration of mast cells to inflamed tissues during an acute-phase response. CRP-mediated recruitment of mast cells might play an important role in hypersensitivity and inflammatory processes.
Fujimoto
Takahiro
T
Department of Anesthesiology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, Japan. tfujimot@highway.ne.jp
Sato
Yoshitaka
Y
Sasaki
Nobuo
N
Teshima
Reiko
R
Hanaoka
Kazuo
K
Kitani
Seiichi
S
D000818:Animals; D002097:C-Reactive Protein / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002633:Chemotaxis / Q000502:physiology*; D004285:Dogs; D005456:Fluorescent Dyes / Q000378:metabolism; D016257:Fura-2 / Q000378:metabolism; D006632:Histamine / Q000378:metabolism; D006737:Horseshoe Crabs / Q000378:metabolism; D006801:Humans; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology*; D034801:Mastocytoma; D037342:Pertussis Toxin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D005456:Fluorescent Dyes; D006632:Histamine; D002097:C-Reactive Protein; D037342:Pertussis Toxin; D002118:Calcium; D016257:Fura-2