Vitellogenin of the cockroach, Leucophaea maderae: nucleotide sequence, structure and analysis of processing in the fat body and oocytes.
A cDNA encoding vitellogenin (Vg) of the cockroach, Leucophaea maderae was cloned and sequenced. The deduced amino acid sequence consisting of 1913 residues (including 15 residues for a putative signal peptide) was obtained. Amino-terminal sequence analysis demonstrated that the pro-Vg was cleaved into four polypeptide 'subunits' following the three consensus RXXR cleavage site sequences, which were secreted as four Vg polypeptides (apparent molecular weights = 112-, 100-, 92- and 55-kD), sequestered, and deposited in the egg as four respective vitellin (Vn) polypeptides. There was, however, an additional 90-kD Vn polypeptide existed in the egg. We show that this polypeptide is a processed product from 92-kD Vn polypeptide. Northern blot analysis of poly (A)+ RNA reveals that mRNA coding for Vg is present only in the female fat body cells but neither in the ovary nor in the male fat body cells. The deduced amino acid sequence contained a serine-rich stretch at the C-terminal region. This stretch occurred also in Vgs of Periplaneta americana (Vg1 and Vg2) and Blattella germanica. The Vg of L. maderae had 26% and 31% homology with those of P. americana (Vg1 and Vg2) and B. germanica, respectively. Phylogenetic analysis (neighbour-joining) was made using four cockroach Vgs and the tree was compared with other molecular and conventional phylogenetic trees.
Tufail
Muhammad
M
Division of Molecular Science, Graduate School of Science and Technology, Kobe University, Nada, Kobe 657-8501, Japan.
Takeda
Makio
M
D000273:Adipose Tissue / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003058:Cockroaches / Q000145:classification / Q000235:genetics* / Q000502:physiology; D017931:DNA Primers; D005260:Female; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry; D014819:Vitellogenins / Q000737:chemistry / Q000235:genetics*
D017931:DNA Primers; D019476:Insect Proteins; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D011994:Recombinant Proteins; D014819:Vitellogenins