[Study on the relation between the cell cycle regulators and laryngeal carcinogenesis].
OBJECTIVE
To investigate the clinicopathological significance of the Cyclin E, p21WAF1/CIP1 and p53 expression in laryngeal carcinogenesis.


METHODS
The expression of Cyclin E, p21WAF1/CIP1 and p53 was examined by using immunohistochemical method in normal epithelia (20), dysplastic epithelia (40) and laryngeal cancers (60).


RESULTS
1. In normal epithelium, dysplastic squamous epithelium and in squamous cell carcinoma of the larynx, the positive immunostaining rate of Cyclin E protein was 5.0%(1/20), 20.0%(8/40) and 45.0%(27/60) respectively (P < 0.001), and p21WAF1/CIP1 protein was 95.0%(19/20), 75.0%(30/40) and 63.3%(38/60) respectively (P < 0.01), while the rate of p53 was 0, 30.0%(12/40) and 61.7%(37/60)(P < 0.001) respectively. 2. p21WAF1/CIP1 positive expression in highly, moderately and poorly differentiated carcinomas were 76.2%(16/21), 66.5%(19/29) and 30.0%(3/10) respectively (P < 0.05), p21WAF1/CIP1 protein expression was significantly associated with tumour cell differentiation. 3. The positive expression of Cyclin E closely related with the positive expression of p53 (P < 0.05). No correlation was found between p21WAF1/CIP1 expression and p53 expression.


CONCLUSIONS
1. Cyclin E and p53 could be an early molecular event in the carcinogenesis of larynx. 2. p21WAF1/CIP1 expression is associated with tumour cell differentiation. 3. The mutual mechanism of cell cycle regulators (Cyclin E, p21WAF1/CIP1 and p53) play an important role in the laryngeal carcinoma.
Wang
J
J
Department of Otolaryngology, First People's Hospital, Jining 272111.
Lou
W
W
Dong
M
M
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002453:Cell Cycle; D019927:Cyclin E / Q000096:biosynthesis* / Q000502:physiology; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000096:biosynthesis* / Q000502:physiology; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000502:physiology
C493956:CDKN1A protein, human; D019927:Cyclin E; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D016159:Tumor Suppressor Protein p53