[Pilot study on mutations of p53 gene in laryngeal carcinoma].
OBJECTIVE
To determine the characteristic and frequency of the point mutation of p53 gene.


METHODS
22 cases of laryngeal squamous cell carcinoma (LSCC) with positive expression of mutant p53 protein and 2 cases of normal laryngeal epithelium as control were examined by means of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP), single strand conformation polymorphism (SSCP) and DNA sequencing analyses.


RESULTS
3 cases of LSCC had the point mutation on codons 248, 250 and 254 of exon 7 in p53 gene. None of the cases had the point mutation on codon 249.


CONCLUSIONS
These findings suggest that the point mutation on codon 249 of exon 7 in p53 gene may be uncommon in LSCC. SSCP and DNA sequencing analyses are sensitive and rapid methods for detecting p53 gene mutation.
Zhou
G
G
Department of Otolaryngology, First Affiliated Hospital, WCUMS, Chengdu 610041, China.
Zhang
X
X
Lui
S
S
Lin
D
D
Liang
C
C
Yang
X
X
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D005091:Exons; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA