Identification and polymorphism of Plasmodium vivax RBP-1 peptides which bind specifically to reticulocytes.
Plasmodium vivax merozoite preferentially invades reticulocytes probably using PvRBP-1 as ligand. One hundred and ninety-five, 15-mer peptides has been synthesised from PvRBP-1 sequence; tested in reticulocyte- or erythrocyte-binding assays. Twenty-five peptides (K(d)=76-380 nM) specifically defined four reticulocyte-binding regions. It has been reported that a highly conserved Region-I recombinant fragment binds specifically to reticulocytes. HABP-critical residues for reticulocyte-binding were highly conserved in 20 Colombian P. vivax clinical isolates, suggesting an important biological function. There were six overlapping reticulocyte-binding sites for these peptides according to enzyme sensitivity and mutual competition-binding assays; located on 26- and 41-kDa reticulocyte membrane surface proteins.
Urquiza
Mauricio
M
Fundacion Instituto de Inmunologia de Colombia and Universidad Nacional de Colombia, Cra 50 No 26-00, Bogotá, Colombia SA. mauriciourquiza@yahoo.com
Patarroyo
Manuel A
MA
Marí
Viviana
V
Ocampo
Marisol
M
Suarez
Jorge
J
Lopez
Ramses
R
Puentes
Alvaro
A
Curtidor
Hernando
H
García
Javier
J
Rodríuez
Luis E
LE
Vera
Ricardo
R
Torres
Angela
A
Laverde
Marilu
M
Robles
Ana P
AP
Patarroyo
Manuel E
ME
D000818:Animals; D001665:Binding Sites / Q000235:genetics / Q000502:physiology; D004912:Erythrocytes / Q000378:metabolism; D007700:Kinetics; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D010966:Plasmodium vivax / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D012156:Reticulocytes / Q000378:metabolism*
D008565:Membrane Proteins; D010455:Peptides; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; C075483:reticulocyte-binding protein, Plasmodium vivax