title: Novel fluorophores for single-molecule imaging.
abstract: Nonlinear optical chromophores based on dicyanodihydrofuran acceptors paired with amine donors have been found to exhibit sufficiently large fluorescence quantum yields and stability to enable single-molecule detection in polymeric hosts. To illustrate the breadth of this class, six fluorophores are presented, spanning the emission range from 505 to 646 nm. In contrast to conventional single-molecule fluorophores, the new molecules feature sensitivity to local rigidity, large ground-state dipole moments, and large polarizability anisotropies, properties that can be used to design new reporter experiments at the single-molecule level.
authors.affiliation: Department of Chemistry, Stanford University, Stanford, California 94305, USA.
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Willets
authors.forename: Oksana
authors.initials: O
authors.lastname: Ostroverkhova
authors.forename: Meng
authors.initials: M
authors.lastname: He
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Twieg
authors.forename: W E
authors.initials: WE
authors.lastname: Moerner
mesh_terms: D000588:Amines / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005470:Fluorometry; D005663:Furans / Q000737:chemistry*; D010777:Photochemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000588:Amines; D005456:Fluorescent Dyes; D005663:Furans