title: Interleukin-10 gene promoter region polymorphism is associated with eosinophil count and circulating immunoglobulin E in adult asthma.
abstract: BACKGROUND
  IL-10 has several functional effects relevant to asthma. It can modulate IgE production and induce apoptosis in eosinophils. Polymorphisms of IL-10 gene have been shown to affect IL-10 production.
  
  
  OBJECTIVE
  To establish whether IL-10 polymorphisms are associated with asthma and phenotype-related characteristics.
  
  
  METHODS
  The frequency of three single base exchange polymorphisms (at positions - 1082, - 819 and - 592) and corresponding haplotypes of the IL-10 gene were analysed in 245 adult asthmatic subjects and 405 controls using PCR and restriction fragment length polymorphism (RFLP). The data were assessed for correlations with the eosinophil count, serum IgE and lung function.
  
  
  RESULTS
  The IL-10 haplotype frequencies were similar in asthmatics and controls. Eosinophil count median was 2.0- to 3.2-fold higher among asthmatics with rare ATA/ATA genotype than in asthmatics with other genotypes. No such difference was seen in the control group. When analysed by IL-10 haplotype carrier state and gender, male asthmatics with ATA haplotype had 2.8-fold higher serum IgE than those without ATA. A converse association was found in male controls with ATA haplotype, who had 1.9-fold lower serum IgE than their ATA-negative counterparts. The high IL-10-producing GCC haplotype was associated with impaired lung function in smoking male controls while in asthmatics no clear effect on lung function was found with any of the haplotypes studied.
  
  
  CONCLUSIONS
  These results suggest that the eosinophil counts and serum IgE are differently regulated by IL-10 genotype in asthmatic and in normal subjects. However, IL-10 polymorphism is not related to susceptibility in asthma.
authors.affiliation: Department of Respiratory Medicine, Tampere University Hospital, Tampere, Finland. jussi.karjalainen@uta.fi
authors.forename: J
authors.initials: J
authors.lastname: Karjalainen
authors.forename: J
authors.initials: J
authors.lastname: Hulkkonen
authors.forename: M M
authors.initials: MM
authors.lastname: Nieminen
authors.forename: H
authors.initials: H
authors.lastname: Huhtala
authors.forename: A
authors.initials: A
authors.lastname: Aromaa
authors.forename: T
authors.initials: T
authors.lastname: Klaukka
authors.forename: M
authors.initials: M
authors.lastname: Hurme
mesh_terms: D000368:Aged; D001249:Asthma / Q000235:genetics / Q000276:immunology* / Q000503:physiopathology; D016022:Case-Control Studies; D004804:Eosinophils / Q000276:immunology; D005260:Female; D006239:Haplotypes; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D016753:Interleukin-10 / Q000235:genetics*; D007958:Leukocyte Count; D008168:Lung / Q000276:immunology / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012907:Smoking
chemical_list: D016753:Interleukin-10; D007073:Immunoglobulin E