title: Laminin-5 gamma 2 chain is colocalized with gelatinase-A (MMP-2) and collagenase-3 (MMP-13) in odontogenic keratocysts.
abstract: BACKGROUND
  Odontogenic keratocyst (KC) differs from other epithelial odontogenic cysts in regard to increased epithelial proliferation and a strong tendency to recur. Laminin-5 (Ln-5) is an epithelial anchoring filament component, which after modulation by certain matrix metalloproteinases (MMPs), like MMP-2 and MMP-13, induces epithelial cell migration.
  
  
  METHODS
  Using in situ hybridization and immunohistochemistry, we studied the Ln-5 gamma-2 chain expression related to the expression of MMP-2, -8, and -13 in different odontogenic cysts, including radicular cysts (RC; n = 11), follicular cysts (FC; n = 11), and odontogenic keratocysts (KC; n = 16).
  
  
  RESULTS
  Ln-5 mRNA was present in all cysts examined, while less than half of KCs and RCs (33 and 40%, respectively) demonstrated MMP-2 mRNA. MMP-13 mRNA was present in all KC samples. Ln-5 protein was located as a continuous ribbon in BM zone of all KCs, and MMP-2 and MMP-13 immunoreactivities colocated significantly with Ln-5 in that area. MMP-8 was expressed by stromal macrophages and epithelial goblet cells, but never located in BM zone.
  
  
  CONCLUSIONS
  Our results indicate that the colocalization of Ln-5 with MMP-2 or MMP-13, but not with MMP-8, in BM zone of KCs, may be related to special characteristics of KC.
authors.affiliation: Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland.
authors.forename: Jaana
authors.initials: J
authors.lastname: Wahlgren
authors.forename: Anu
authors.initials: A
authors.lastname: Väänänen
authors.forename: Olli
authors.initials: O
authors.lastname: Teronen
authors.forename: Timo
authors.initials: T
authors.lastname: Sorsa
authors.forename: Emma
authors.initials: E
authors.lastname: Pirilä
authors.forename: Jarkko
authors.initials: J
authors.lastname: Hietanen
authors.forename: Päivi
authors.initials: P
authors.lastname: Maisi
authors.forename: Leo
authors.initials: L
authors.lastname: Tjäderhane
authors.forename: Tuula
authors.initials: T
authors.lastname: Salo
mesh_terms: D001485:Basement Membrane / Q000737:chemistry / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000378:metabolism*; D002465:Cell Movement; D017364:Collagenases / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D053509:Matrix Metalloproteinase 13; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020784:Matrix Metalloproteinase 8 / Q000378:metabolism; D009807:Odontogenic Cysts / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D021122:Protein Subunits / Q000032:analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D018709:Statistics, Nonparametric; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D017364:Collagenases; D053509:Matrix Metalloproteinase 13; D020778:Matrix Metalloproteinase 2; D020784:Matrix Metalloproteinase 8; D021122:Protein Subunits; D012333:RNA, Messenger; D000097794:Kalinin; C507079:MMP13 protein, human