title: [Isolation and disruption analysis of a developmental gene of Myxococcus xanthus].
abstract: FruB is an associated protein of FruA, a transcription factor essential for the development of Myxococcus xanthus. Degenerate oligonucleotide primers were designed based on the N-terminal amino acid sequence of FruB. Using genomic DNA as template, an approximate 110 bp fragment was generated and further served as a probe to screen a small genomic library of M. xanthus. A 4.5 kb SacI fragment was isolated on the basis of its homology to the probe. Disruption of fruB delayed the morphogenesis of fruiting bodies and depressed spore yield, suggesting that to some extent, FruB might be involved in the development of M. xanthus.
authors.affiliation: Department of Biochemistry, Southeast University Medical College, Nanjing 210009, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Mao
authors.forename: Daoyong
authors.initials: D
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016941:Myxococcus xanthus / Q000235:genetics* / Q000254:growth & development; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C104848:FruA protein, Myxococcus xanthus; D014157:Transcription Factors