title: [The foundation of the injury model on cultured human fetal lung fibroblasts and its immunohistochemistry research].
abstract: Cultured human fetal lung Fbs was first used to study the relationship between wound age and the change of cFn synthesized by Fbs in Vitro. A sterilized injector was applied to scrap the cultured confluent Fbs and a "wound" was obtained. Fbs around the wound was seen to change from a stationary stage to an active stage. Using the method of immunochemistry and image analysis system (IAS), cFn synthesized by Fbs around the wound was observed at once, 30 min, 1 h, 3 h and 6 h after injury. It was found that cFn began to change 1 h after injury and increased continually within 6 h. It suggests that the change of cFn synthesized by Fbs is time-dependent after injury and this approach breaks a new path in the study on estimation of wound age.
authors.affiliation: Department of Forensic Medicine, Shanghai Medical University, Shanghai 200032, P. R. China.
authors.forename: Y W
authors.initials: YW
authors.lastname: Shen
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Zhao
authors.forename: Y J
authors.initials: YJ
authors.lastname: Chen
authors.forename: Z K
authors.initials: ZK
authors.lastname: Yan
authors.forename: Y J
authors.initials: YJ
authors.lastname: Gu
authors.forename: W Q
authors.initials: WQ
authors.lastname: Wu
authors.forename: Y P
authors.initials: YP
authors.lastname: Liao
mesh_terms: D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D005353:Fibronectins / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D013997:Time Factors; D014945:Wound Healing
chemical_list: D005353:Fibronectins