Molecular characterization of bovine enteric caliciviruses: a distinct third genogroup of noroviruses (Norwalk-like viruses) unlikely to be of risk to humans.
Bovine enteric caliciviruses (BoCVs) have been classified in the Norovirus (Norwalk-like virus) genus of the Caliciviridae, raising questions about zoonotic transmission and an animal reservoir for the human Norwalk-like viruses (NLVs), an important cause of nonbacterial gastroenteritis in humans. We examined the genetic relationship of human NLVs to BoCVs that were identified by using reverse transcription-PCR with primer pairs originally designed to detect human NLVs. Polymerase, capsid, and open reading frame 3 (ORF3) gene sequence analyses of BoCVs that were identified from 1976 to 2000 from throughout the United Kingdom showed that BoCVs formed a distinct third genogroup of closely related viruses distinct from the human genogroup I and II NLVs. Evidence was not obtained to support the concept that BoCVs are circulating in humans and pose a threat to human health.
Oliver
S L
SL
Department of Pathology & Infectious Diseases, Royal Veterinary College, Royal College Street, London NW1 0TU, United Kingdom.
Dastjerdi
A M
AM
Wong
S
S
El-Attar
L
L
Gallimore
C
C
Brown
D W G
DW
Green
J
J
Bridger
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D017250:Caliciviridae Infections / Q000662:veterinary* / Q000821:virology; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification* / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D015047:Zoonoses
D036022:Capsid Proteins; D014764:Viral Proteins