title: X-ray crystallographic characterization and phasing of a fucose-specific lectin from Aleuria aurantia.
abstract: A fucose-specific lectin from Aleuria aurantia was crystallized in its native form and was also cocrystallized with HgCl(2). Crystallization was performed using the sitting-drop vapour-diffusion method with ammonium sulfate as a precipitant. Both the Hg-free native crystals and the Hg cocrystals belong to the hexagonal space group P6(5)22. The unit-cell parameters for the Hg-free form are a = 84.0, c = 250.1 A and for the Hg cocrystals are a = 83.9, c = 254.3 A. Both forms of the crystals diffract X-rays to 2.3 A resolution and are suitable for high-resolution crystal structure determination. Initial phasing was successfully performed by the MAD method using the Hg cocrystals and the electron density obtained was good enough for model building.
authors.affiliation: Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fujihashi
authors.forename: Diane H
authors.initials: DH
authors.lastname: Peapus
authors.forename: Eijiro
authors.initials: E
authors.lastname: Nakajima
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Yamada
authors.forename: Jun Ichi
authors.initials: JI
authors.lastname: Saito
authors.forename: Akiko
authors.initials: A
authors.lastname: Kita
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Higuchi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Sugawara
authors.forename: Akikazu
authors.initials: A
authors.lastname: Ando
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kamiya
authors.forename: Yoshiho
authors.initials: Y
authors.lastname: Nagata
authors.forename: Kunio
authors.initials: K
authors.lastname: Miki
mesh_terms: D001203:Ascomycota / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods; D005656:Fungal Proteins / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*; D008627:Mercuric Chloride / Q000737:chemistry; D055672:Static Electricity
chemical_list: D005656:Fungal Proteins; D037102:Lectins; C064549:lectin, Aleuria aurantia; D008627:Mercuric Chloride