[Analysis of the composition in fructooligosaccharide by high performance liquid chromatography].
In this paper, an analytical method for determination of fructooligosaccharide by high performance liquid chromatography(HPLC) is presented. The HPLC conditions are mu-Bondapak C18 column, 300 mm x 3.9 mm i.d., water as mobile phase and refractive index detector. Monosaccharide, disaccharide, trisaccharide, tetrasaccharide, pentasaccharide, hexasaccharide etc. can be simultaneously determined in a single injection. The method has been used to determine the fructooligosaccharides in "Boai biogen". The results are satisfactory. The CVs were 0.55%-1.1% and correlation coefficients were 0.9992-1.000. The lowest detectable limit for fructooligosaccharide was in microgram level.
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Health and Epidemic Prevention Centre of Guangxi Zhuang Autonomous Region, Nanning, 530021.
D002851:Chromatography, High Pressure Liquid; D005632:Fructose / Q000032:analysis*; D009844:Oligosaccharides / Q000737:chemistry*; D013395:Sucrose / Q000032:analysis*; D014312:Trisaccharides / Q000032:analysis*
D009844:Oligosaccharides; D014312:Trisaccharides; C116580:fructooligosaccharide; D005632:Fructose; D013395:Sucrose