[Studies on DNA-DNA hybridization and 16S rDNA sequence of rhizobia isolated from Shapotou desert soil in Ningxia autonomous region of China].
Based on the previous studies on numerical taxonomy and multilocus enzymes electrophoresis patterns of the 12 rhizobial strains isolated from Shapotou region, the contents of G + C mol%, DNA-DNA relatedness and 16SrDNA sequence of the representative strain were tested. The DNA G + C content of the members of this group ranged from 56.4 to 62.2. The values of DNA-DNA hybridization within the group were above 70%, and relatendness between representative strains of this group and known rhizobial species was below 66.6%. The full-length of 16S rDNA sequence of representative strain N220 was compared with the type strains of all known rhizobia species and related bacteria by the PHLIP version 3.572c composed a unrooted phylogenetic tree, the strain N220, R. galegea, two unnamed rhizobial strains(SH19312, SH22623) and three A-grobacterium strains constituted a branch in this tree. The similarity values of 16S rDNA sequence between strain N220 and other strains in this branch were above 95%.
Li
Y
Y
College of Biological Sciences, China Agricultural University, Beijing 100094.
Bai
Y
Y
Chen
W
W
D001483:Base Sequence; D002681:China; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D007887:Fabaceae / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S