[In vitro culture of modified neonatal pig islets].
A cell-isolation-and-cell-culture-in-vitro method which can produce a mass of new-born pig islet-like clusters was introduced. The new-born pig pancreas were incubated in V-type collagenase (0.5-1.0 mg.ml-1) for 8-12 minutes, and then cultured in vitro for 7 days before large amount of highly purified porcine islets were produced. The neonatal pig islets had good insulin secretion and normal sub-cell structure. The results suggest that the neonatal pig islets are probably a fine donor source for clinical xenotransplantation of islets.
Luo
X M
XM
Department of Radiology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Zhu
H C
HC
Ye
B
B
Wan
W
W
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005260:Female; D007328:Insulin / Q000096:biosynthesis*; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D008297:Male; D013552:Swine
D007328:Insulin