title: Presence of the new human metapneumovirus in French children with bronchiolitis.
abstract: We developed a polymerase chain reaction (PCR) to detect human metapneumovirus (hMPV) in French children hospitalized for acute respiratory tract disease during the winter of 2001 to 2002. This assay showed that 19 (6.6%) of 337 respiratory specimens that were negative for other respiratory viruses were positive for hMPV. Seven hMPV infections were also detected in the previous winter. The patients had the following clinical syndromes: bronchiolitis, 16; acute exacerbation of asthma, 4; bronchitis, 1; laryngitis, 1; high fever with diarrhea, 2; high fever with rhinopharyngitis, 1; rhinopharyngitis with conjunctivitis and otitis, 1. The sequences of the seven amplified products were similar for all cases and had 99% homology with the previously described N gene sequence.
authors.affiliation: Laboratory of Human and Molecular Virology, Caen University Hospital, France.
authors.forename: François
authors.initials: F
authors.lastname: Freymouth
authors.forename: Astrid
authors.initials: A
authors.lastname: Vabret
authors.forename: Loic
authors.initials: L
authors.lastname: Legrand
authors.forename: Nicolas
authors.initials: N
authors.lastname: Eterradossi
authors.forename: Françoise
authors.initials: F
authors.lastname: Lafay-Delaire
authors.forename: Jacques
authors.initials: J
authors.lastname: Brouard
authors.forename: Bernard
authors.initials: B
authors.lastname: Guillois
mesh_terms: D001988:Bronchiolitis / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D002648:Child; D004196:Disease Outbreaks; D005260:Female; D005602:France / Q000453:epidemiology; D006801:Humans; D007223:Infant; D008297:Male; D029121:Metapneumovirus / Q000302:isolation & purification* / Q000472:pathogenicity; D018184:Paramyxoviridae Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D017385:Sequence Homology