[Novel affinity membrane used for bilirubin removal].
Bilirubin is a toxic substance. In order to effectively remove it from the hepatic patients' blood, two novel affinity membranes were prepared. These were prepared by chemically grafting on cellulose and immobilized with different ligands. One kind of ligand was poly-D-lysine, the other one was quaternary ammonium salt. Both affinity membranes were used for removal bilirubin from phosphate buffer and HSA solutions, and the effects of temperature, HSA concentration, adsorption time in static state experiment and flow rate in dynamic state experiment have been investigated. The results indicated that the membranes could remove over 70% bilirubin from phosphate buffer and at least 50% from low concentration HSA solutions. The results also indicated that the removal efficiency was better at higher temperature. In the static state experiment, four hours can be selected as adsorption time. In the dynamic state experiment, the flow rate can be properly increased.
Wei
G L
GL
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, China.
Shang
Z H
ZH
Yu
Y N
YN
Liu
X L
XL
Gao
Z H
ZH
Pan
M C
MC
D000042:Absorption; D001663:Bilirubin / Q000097:blood* / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008567:Membranes, Artificial; D009994:Osmolar Concentration; D012709:Serum Albumin / Q000737:chemistry* / Q000302:isolation & purification
D008567:Membranes, Artificial; D012709:Serum Albumin; D001663:Bilirubin