title: Clinical isolates of Enterobacteriaceae producing extended-spectrum beta-lactamases: prevalence of CTX-M-3 at a hospital in China.
abstract: The prevalence of extended-spectrum beta-lactamase-producing strains was demonstrated in 5 of 44 (11.4%) Escherichia coli, 17 of 43 (39.5%) Klebsiella pneumoniae, 3 of 50 (6.0%) Enterobacter cloacae, and 2 of 25 (8.0%) Citrobacter freundii strains at a teaching hospital in China. Nineteen of these 27 strains expressed CTX-M-3 beta-lactamase (pI 8.6). A subset of the clinical isolates expressing the CTX-M-3 enzyme, tested by pulsed-field gel electrophoresis, revealed multiple clones. Five isolates expressed a novel enzyme, SHV-43 (pI 8.0), which had two substitutions (Leu113Phe and Thr149Ser) compared with SHV-1.
authors.affiliation: Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Swathi
authors.initials: S
authors.lastname: Kelkar
authors.forename: Weiyuan
authors.initials: W
authors.lastname: Wu
authors.forename: Minjun
authors.initials: M
authors.lastname: Chen
authors.forename: John P
authors.initials: JP
authors.lastname: Quinn
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002681:China; D016521:Electrophoresis, Gel, Pulsed-Field; D004755:Enterobacteriaceae / Q000201:enzymology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; C456079:beta-lactamase CTX-M-13; D001618:beta-Lactamases