title: Endogenous substance P in corneal epithelial cells and keratocytes.
abstract: OBJECTIVE
  To detect endogenous substance P (SP) and neurokinin receptor 1 (NK1R) in cultured human corneal epithelial cells (HE) and cultured human keratocytes (HK).
  
  
  METHODS
  Messenger RNA (mRNA) expression of SP and endogenous SP in HE and HK was investigated by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay. mRNA expression of NK1R in HE and HK was investigated by RT-PCR.
  
  
  RESULTS
  The mRNA expression of SP and endogenous SP were recognized in HE and HK. Furthermore, the mRNA of NK1R was expressed in HE and HK.
  
  
  CONCLUSIONS
  It was suggested that endogenous SP regulates the biological functions of HE and HK in autocrine or paracrine fashion.
authors.affiliation: Department of Ophthalmology, Asama Municipal Hospital, Nagano, Japan.
authors.forename: Michifumi
authors.initials: M
authors.lastname: Watanabe
authors.forename: Kiyo
authors.initials: K
authors.lastname: Nakayasu
authors.forename: Minoru
authors.initials: M
authors.lastname: Iwatsu
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kanai
mesh_terms: D000368:Aged; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology*; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D018040:Receptors, Neurokinin-1 / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013373:Substance P / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D018040:Receptors, Neurokinin-1; D013373:Substance P