title: High performance liquid chromatography and photodiode array detection of ferulic acid in Rubus protoplasts elicited by O-glycans from Fusarium sp. M7-1.
abstract: So far only little data have been available concerning the eliciting capacity of well defined glycan molecules isolated from plant pathogens. This study brings new information about changes in plant cells caused by fungal pathogens. Sugar fractions derived from glycoproteins isolated from the fungus Fusarium sp. M7-1 have been tested here as signaling molecules. The ability of three O-glycan fractions (named in this work inducer I, II, III) to trigger responses in Rubus protoplasts has been examined. It was found that inducer III was the most efficient as it elicited changes in the levels of phenylpropanoid pathway intermediates in relation to phenylalanine-ammonia lyase (PAL) activation.
authors.affiliation: Centre de Recherches sur les Macromolécules Végétales-CNRS, associé à l'Université Joseph Fourier, Grenoble, France. mihain@micro.biol.ethz.ch
authors.forename: Mihai
authors.initials: M
authors.lastname: Nita-Lazar
authors.forename: Lionel
authors.initials: L
authors.lastname: Chevolot
authors.forename: Shojiro
authors.initials: S
authors.lastname: Iwahara
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takegawa
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Furmanek
authors.forename: Yvette
authors.initials: Y
authors.lastname: Lienart
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis*; D005670:Fusarium / Q000737:chemistry*; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D011523:Protoplasts / Q000737:chemistry* / Q000187:drug effects*; D027824:Rosaceae / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors
chemical_list: D003373:Coumaric Acids; D011134:Polysaccharides; C004999:ferulic acid; D010650:Phenylalanine Ammonia-Lyase