Allelic imbalance in selected chromosomal regions in ovarian cancer.
Ovarian cancer (OC) is often asymptomatic at the initial stage. When diagnosed, up to 75% of the patients present grade III or IV tumors with metastasis in nearby organs of the abdomen. Genetic imbalance is abundant in OC, and allelic loss (AL) of specific chromosomal regions is considered an early event. To establish association between genetic markers for early diagnosis/prognosis of OC, our target was to define narrow specific regions of AL. We analyzed 65 ovarian carcinomas by using 19 microsatellite markers located in three different chromosomes. First, a 7.6-Mb region containing the estrogen receptor (ESR1) and the tumor suppressor gene LATS1 was analyzed. Several chromosomal breakpoints flanking ESR1 affecting the region harboring LATS1 were found. Second, we found chromosomal breakpoints on 13q13.1 approximately q13.3 that defined two narrow regions flanking the BRCA2 locus. Third, our ovarian tumors exhibited a very high frequency of AL on 16q and chromosomal breakpoints defining two narrow regions within 16q22.2 approximately q24.3. In this article, we report three new polymorphic microsatellite markers and strong evidence of AL of narrow well-defined regions in hot spots on 6q, 13q, and 16q in ovarian tumors.
Hansen
Lise Lotte
LL
Department of Human Genetics, The Bartholin Building, University of Aarhus, DK-8000 C, Aarhus, Denmark. lotte@humgen.au.dk
Jensen
Lise Lind
LL
Dimitrakakis
Constantine
C
Michalas
Stylianos
S
Gilbert
Fred
F
Barber
Hugh R K
HR
Overgaard
Jens
J
Arzimanoglou
Iordanis I
II
D022981:Allelic Imbalance; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D002885:Chromosomes, Human, Pair 16; D002896:Chromosomes, Human, Pair 6; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm; D005819:Genetic Markers