Normal telomere lengths of spermatozoa in somatic cell-cloned bulls.
Interesting questions have been raised regarding cloned animals, including whether cloning restores cellular senescence undergone by donor cells, and how long cloned animals will be able to live. In this study, focusing our attention on the fact that telomere lengths of spermatozoa are longer than those of any somatic cells and that telomere length is maintained throughout aging in humans, we compared the telomere lengths of spermatozoa in normal and two somatic cell-cloned cattle. The telomere lengths of the spermatozoa in the normal cattle (22.42+/-0.32 kb) were maintained throughout aging as in humans. In the cloned cattle, telomere lengths of the spermatozoa (25.8 and 20.9 kb) were the same as or longer than those found in normal cattle. Considering that telomere lengths of the donor cells, which had been derived from the muscle tissue of an old bull, were reported to be 20.1 kb, the results suggested that the telomere lengths of the germ cell line had extended from nucleus transfer to spermatogenesis. Moreover, we produced offspring (nine calves) from a somatic cell-cloned bull and measured the telomere lengths of their leukocytes. In all of the offspring, the telomere lengths of leukocytes were normal, too. These results indicate the possibility that somatic cloned bulls could be used as breeding sires.
Miyashita
Norikazu
N
Laboratory of Animal Cell Biology, National Institute of Agrobiological Sciences, Kukizaki, Ibaraki 305-0901, Japan. nmiya@affrc.go.jp
Shiga
Kazuho
K
Fujita
Tatsuo
T
Umeki
Hidenobu
H
Sato
Wataru
W
Suzuki
Toshiyuki
T
Nagai
Takashi
T
D000818:Animals; D015139:Blotting, Southern / Q000662:veterinary; D002417:Cattle / Q000235:genetics* / Q000502:physiology; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics*; D019976:Cloning, Organism; D004247:DNA / Q000302:isolation & purification; D005260:Female; D006824:Hybridization, Genetic; D007962:Leukocytes; D008297:Male; D013094:Spermatozoa / Q000166:cytology / Q000648:ultrastructure*; D016615:Telomere / Q000648:ultrastructure*
D004247:DNA