[In vitro and in vivo studies on the biologic effects and molecular mechanism of recombinant RA538 and antisense C-myc adenovirus on human gastric, esophageal and cancer cell lines with high-expression of Bcl-2 gene].
In this study, the biological effects and molecular mechanism of recombinant RA538 and antisense c-myc adenovirus on human gastric, esophageal, 2BS and high-expression bcl-2 gene cancer cell lines were studied in vitro and in vivo. The results were as follows: Ad-RA538 and Ad-ASc-myc could strongly inhibit cell growth and induce apoptosis of SGC7901 cells in vitro and in vivo, and could down-regulate expression of c-myc, bcl-2 and cyclinD1 gene, up-regulate expression of bax gene. Ad-RA538 or Ad-AS c-myc could not inhibit cell growth and induce apoptosis changes of EC109, EC8712, 2BS and high-expression bcl-2 gene cancer cell lines, and could not down-regulate expression of c-myc and bcl-2 gene. The results indicated that: Ad-RA538 or Ad-AS c-myc can inhibit growth and induce apoptosis of gastric cancer cell in vitro and in vivo. They relate to c-myc, bcl-2, cyclinD1 and bax gene closely and play a key role on biologic effects in gastric cancer cells. Ad-RA538 and Ad-AS c-myc could not produce relevant changes on esophageal cancer, 2BS and high-expression bcl-2 gene cell lines.
Chen
J P
JP
Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038.
D000260:Adenoviruses, Human / Q000235:genetics*; D016373:DNA, Antisense; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D016373:DNA, Antisense; D019253:Proto-Oncogene Proteins c-bcl-2; D016271:Proto-Oncogene Proteins c-myc; D011994:Recombinant Proteins; D014212:Tretinoin