Quantitative polarized light microscopy.
We describe a simple modification to a confocal microscope, which analyses the state of polarization of light emerging from the specimen so as to permit quantitative polarized light microscopy to be performed. The system uses a novel form of rotating analyser which, together with lock-in detection, permits images to be obtained where the image contrast corresponds to both specimen retardance and orientation (e.g. in the case of a birefringent specimen). Images are presented from a wide range of specimens and the origin of the contrast observed from simple point scatterers is investigated both theoretically and experimentally.
Massoumian
F
F
Department of Engineering Science, University of Oxford, Parks Road, UK.
Juskaitis
R
R
Neil
M A A
MA
Wilson
T
T