[cDNA cloning and sequence analysis of genome segment S7 of rice black-streaked dwarf virus].
Genome segments 7 of zhejiang and Hebei isolates of rice black-streaked dwarf virus (RBSDV) were amplified and sequenced. Segment 7 of Zhejiang isolate was consisted of 2193 nts (EMBL accession no. AJ297427) in length and that of Hebei isolate was 2190 nts (AJ297428). Both segments contained two non-overlapping open reading frames (ORFs), which encoded two polypeptides with molecular weights of 41 kD and 36 kD. These two segments shared 99% nucleotide identity, 100% and 94.4% amino acid identities of ORF1 and ORF2, shared 93.5% and 93.8% identities at nucleotide level, 98.1% (ORF1) and 96.5%/97.8% (ORF2) at amino acid level with S7 of Japanese RBSDV, and shared 85.1% and 85.3% identities at nucleotide level, 92.3% (ORF1), 85.5%/86.8% (ORF2) at amino acid level with S6 of Italian MRDV.
Zhang
Hengmu
H
College of Aagriculture and Biotechnology, Zhejiang University, Virology Laboratory, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Chen
Jianping
J
Lei
Juanli
J
Cheng
Ye
Y
Xue
Qingzhong
Q
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D012275:Oryza / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics* / Q000302:isolation & purification; D012087:Reoviridae / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D018076:DNA, Complementary