title: The influence of acute hypercapnia on the quantity and oxidative metabolism of bronchoalveolar lavage-derived leukocytes in the mechanically ventilated rabbit.
abstract: BACKGROUND
  Permissive hypercapnia exerts a protective influence in tissue injury, possibly by attenuating free radical activity. Increased CO2 concentrations may decrease the production of phagocyte reactive oxygen intermediates (ROI). In this study, we used bronchoalveolar lavage (BAL) to determine whether isooxic permissive hypercapnia due to hypoventilation would influence neutrophil recruitment into alveolar spaces and ROI production of BAL phagocytes, as measured by luminol dependent chemiluminescence.
  
  
  METHODS
  27 Chinchilla rabbits randomized into three groups: control group (C; n=7), normocapnia (NC; n=10), and hypercapnia (HC; n=10) were anaesthetized with pentobarbitone and intubated. Bronchoalveolar lavage was obtained either after intubation (controls) or after mechanical ventilation lasting 240 minutes under normocapnia or permissive hypercapnia conditions. BAL cells were counted and luminol-dependent chemiluminescence estimations were performed.
  
  
  RESULTS
  The BAL neutrophil count was significantly higher in the hypercapnia group than in normocapnia animals. The chemiluminescence of opsonized zymosan-stimulated cells was significantly higher in the normocapnia group than in the controls; the differences between the HC and NC animals, and between the HC and control animals, did not reach statistical significance.
  
  
  CONCLUSIONS
  In the mechanically ventilated rabbit under pentobarbitone anaesthesia, acute isooxic hypercapnia due to hypoventilation increases neutrophil BAL count, without direct impact on mixed BAL-cell chemiluminescence; moreover, conventional ventilation increases the chemiluminescence of opsonized zymosan-stimulated BAL cells
authors.affiliation: Department of Experimental Anaesthesiology, Chair of Anaesthesiology and Intensive Therapy, Medical University, Poznań, Poland. hbillert@am.poznan.pl
authors.forename: Hanna
authors.initials: H
authors.lastname: Billert
authors.forename: Leon
authors.initials: L
authors.lastname: Drobnik
authors.forename: Arystarch
authors.initials: A
authors.lastname: Makowski
mesh_terms: D000818:Animals; D001794:Blood Pressure; D018893:Bronchoalveolar Lavage; D002417:Cattle; D005260:Female; D006863:Hydrogen-Ion Concentration; D006935:Hypercapnia / Q000150:complications* / Q000473:pathology*; D007962:Leukocytes / Q000378:metabolism*; D008163:Luminescent Measurements; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D010586:Phagocytes; D011817:Rabbits; D017382:Reactive Oxygen Species; D013997:Time Factors
chemical_list: D017382:Reactive Oxygen Species; D010100:Oxygen