[Purification of follicle-stimulating hormone by radial-flow chromatography].
By comparing the purification results of radial-flow chromatography with conventional chromatography, a new purification process of follicle-stimulating hormone (FSH) from crude sample of human menopausal gonadotrophin has been developed. The results showed that the specific activity, activity recovery, and purification factor of FSH purified by radial-flow chromatography were higher than those by conventional chromatography in large scale. The specific activity of FSH purified was 180 IU/mg protein, activity recovery 56%, purification factor 150. The purified product meets the quality requirement of the Standard of Chinese Pharmacopoeia.
Guo
L A
LA
Central Labaratory, 4th Military Medical University, Xi'an 710032, China. lianguo@fmmu.edu.cn
Yan
Z
Z
Yang
X D
XD
Chu
X Q
XQ
Xu
H F
HF
D002852:Chromatography, Ion Exchange / Q000379:methods*; D005640:Follicle Stimulating Hormone / Q000302:isolation & purification*; D008596:Menotropins
D008596:Menotropins; D005640:Follicle Stimulating Hormone