Crystallization and preliminary X-ray diffraction studies of alpha-cyclodextrin glucanotransferase isolated from Bacillus macerans.
Single crystals of alpha-cyclodextrin glucanotransferase isolated from Bacillus macerans have been grown with polyethylene glycol 6000 as a precipitating agent by sitting-drop vapour diffusion at room temperature. The crystals were suitable for X-ray analysis and diffracted to at least 2.0 A (space group P2(1)2(1)2(1)), with unit-cell parameters a = 66.79 (2), b = 79.66 (1), c = 141.16 (1) A. Assuming the asymmetric cell to be occupied by a monomer of 74 kDa, the unit cell contains 42.6% solvent with a crystal Volume per protein mass, V(M), of 2.53 A(3) Da(-1).
Choe
Hui Woog
HW
Department of Chemistry, College of Natural Science, Chonbuk National University, 561-756 Chonju, South Korea. hwchoe@moak.chonbuk.ac.kr
Park
Kyo Sun
KS
Labahn
Jörg
J
Granzin
Joachim
J
Kim
Cheol Ju
CJ
Büldt
Georg
G
D001407:Bacillus / Q000201:enzymology*; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D005964:Glucosyltransferases / Q000737:chemistry* / Q000378:metabolism
D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase