title: Paracrine communication between malignant and non-malignant prostate epithelial cells in culture alters growth rate, matrix protease secretion and in vitro invasion.
abstract: Epithelial cancer cell invasion is facilitated by stromal cells, immune cells, endothelial cells and other epithelial cells. We have used two human papilloma immortalized prostate cell lines, CA-HPV-10 from a carcinoma and PZ-HPV-7 cells from normal prostatic epithelium to study cell-cell influences on growth, gelatinase secretion, invasion and responses to TGFbeta1. We found that co-culture with CA-10 carcinoma cells stimulates proliferation of the PZ-7 epithelial line. TGFbeta1 inhibited growth of both lines, but while inhibitory effects on the CA-10 cells diminished after removal of the peptide, inhibition of PZ-7 was lasting. Interestingly, the TGFbeta-induced growth inhibition in PZ-7 cells could be partially reversed by co-culture with CA-10 cells. Co-culture with CA cells in a 3-chamber invasion assay also promoted invasion of PZ cells. CA-10 invasion was enhanced by co-culture with TGFbeta1-treated-PZ-7 cultures and this enhancement was associated with TGFbeta1-induced secretion of matrix metalloproteinase-9. Our observations suggest that interaction between prostate cancer cells and prostate epithelial cells may promote proliferation of the epithelial cell population and produce a paracrine source of MMP-9 which may facilitate early cancer cell invasion.
authors.affiliation: Department of Pharmaceutical Sciences, North Dakota State University, Fargo, North Dakota, USA.
authors.forename: Andrea H
authors.initials: AH
authors.lastname: Greiff
authors.forename: William M
authors.initials: WM
authors.lastname: Fischer
authors.forename: Inder
authors.initials: I
authors.lastname: Sehgal
mesh_terms: D002450:Cell Communication; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D006801:Humans; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D011467:Prostate / Q000166:cytology / Q000473:pathology / Q000503:physiopathology*; D011471:Prostatic Neoplasms / Q000473:pathology / Q000503:physiopathology*; D014407:Tumor Cells, Cultured
chemical_list: D017077:Culture Media, Conditioned; D020782:Matrix Metalloproteinases