Simultaneous determination of oxidative hair dye p-phenylenediamine and its metabolites in human and rabbit biological fluids.
A liquid chromatography with an electrochemical detector method has been developed for the quantitative measurement for three diamine derivatives (p-phenylenediamine, N,N(')-p-phenylenebisacetamide, and 4-aminoacetanilide) in human urine and rabbit blood, urine, and feces. The detection cell consisted of a glassy carbon electrochemical signal obtained with a supporting electrolyte containing 20% methanol-5mM octylammonium orthophosphate (pH 6.30) as the mobile phase. A comparison of the results obtained from HPLC-UV shows agreement.
Wang
Lai-Hao
LH
Department of Applied Chemistry, China Nan University of Pharmacy and Science, Tainan 71743, Taiwan, ROC. e201466@ms47.hinet.net
Tsai
Shu-Jen
SJ
D000083:Acetanilides / Q000032:analysis / Q000097:blood / Q000378:metabolism / Q000652:urine; D000328:Adult; D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000737:chemistry; D005260:Female; D006202:Hair Dyes / Q000032:analysis* / Q000378:metabolism*; D006801:Humans; D008297:Male; D010084:Oxidation-Reduction; D010655:Phenylenediamines / Q000032:analysis* / Q000097:blood / Q000378:metabolism* / Q000652:urine; D011817:Rabbits; D012680:Sensitivity and Specificity
D000083:Acetanilides; D006202:Hair Dyes; D010655:Phenylenediamines; C519644:4-aminoacetanilide