title: Study on the retention behaviour of metal-tetraphenyl porphine chelates in reversed-phase high performance liquid chromatography.
abstract: The retention behaviour of metal chelates of alpha,beta,gamma,delta-tetraphenylporphine (TPP) was studied in reversed-phase high performance liquid chromatography (RP-HPLC). On C18 column, with various organic solvents as mobile phases, the retention of the chelates tends to increase in the following order: ZnTPP < TPP < NiTPP < CuTPP. This retention behaviour can get a good explanation from Horvath's "solvophobic theory". With the assumption that the electrostatic field strength of metal chelates can be measured by the ratio of electronegativity vs. ionic radius (EN/ri) of central metal ion, EN/ri was established to be an index for the retention behaviour of metal-TPP chelates. It was found that lnk' is in good linear relationship with the EN/ri value.
authors.affiliation: Research Center of Physical and Chemical Analysis, Hebei University, Baoding 071002, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Shi
authors.forename: Y H
authors.initials: YH
authors.lastname: Wang
authors.forename: C G
authors.initials: CG
authors.lastname: Fu
mesh_terms: D002614:Chelating Agents / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D007502:Iron Chelating Agents / Q000302:isolation & purification; D008665:Metalloporphyrins / Q000302:isolation & purification*; D011166:Porphyrins / Q000302:isolation & purification*
chemical_list: D002614:Chelating Agents; D007502:Iron Chelating Agents; D008665:Metalloporphyrins; D011166:Porphyrins; C076448:zinc tetraphenylporphine; C049692:tetraphenylporphine