[Studies on ABA-induced H2O2 in Vicia guard cells by means of confocal laser scanning microscopy].
The methods of confocal laser scanning microscopy (CLSM) and microinjection were used to study ABA-induced H2O2 in guard cells (Vicia faba), which were labeled with H2O2 specific probe-2, 7-dichlorofluorescin diacetate(H2DCFDA). The results indicated 100 U/mL catalase (CAT) could inhibit partly stomatal closure induced by ABA. 10(-3) mmol/L ABA could significantly induce H2O2 production in chloroplast in guard cells of Vicia faba following microinjection, and 100 U/mL CAT could partly abolish the effects following simultaneous microinjection of ABA and CAT. These suggest that H2O2 is possibly involved in ABA signaling leading to stomatal closure.
Zhang
X
X
College of Life Sciences, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100.
Zhang
L
L
An
G Y
GY
Gao
J F
JF
Song
C P
CP
D000040:Abscisic Acid / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008845:Microinjections; D018613:Microscopy, Confocal; D031307:Vicia faba / Q000187:drug effects / Q000378:metabolism*
D000040:Abscisic Acid; D006861:Hydrogen Peroxide; D002374:Catalase