[Separation of saccharides in tobacco by capillary zone electrophoresis].
A method for the separation of derivatized saccharides in tobacco with high performance capillary electrophoresis and ultraviolet spectrophotometry (HPCE-UV) is presented. Five saccharides in tobacco were derivatized with p-aminobenzonitrile, separated at pH 10.5 with 50 mmol/L borate buffer containing 5% (volume fraction, the same in the following percentage concentration) methanol, 5% acetonitrile, 2.5% ethyleneglycol, 2.5% 2-propanol and 1 mmol/L sodium dodecyl sulfate, and detected at 285 nm.
Ma
Qiang
Q
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
He
You-zhao
YZ
Xiao
Xie-zhong
XZ
Huang
Rui
R
Xu
Hai-tao
HT
D019075:Electrophoresis, Capillary / Q000379:methods*; D005947:Glucose / Q000302:isolation & purification; D008320:Maltose / Q000302:isolation & purification; D009005:Monosaccharides / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D014026:Nicotiana / Q000737:chemistry*; D014994:Xylose / Q000302:isolation & purification
D009005:Monosaccharides; D008320:Maltose; D014994:Xylose; D005947:Glucose