[Quantitative determination of icariin in "tangzhi shuangjiang cha" by high performance liquid chromatography].
Icariin in "Tangzhi Shuangjiang Cha" was quantitatively determined by HPLC with ODS column, a mixture of MeOH-H2O(60:40, V/V) as mobile phase and UV detection at 270 nm. There was a good linear relationship within the range of 0.1 g/L-0.5 g/L(r = 0.9993). The average recovery was 101.4%, RSD was below 2.7%(n = 3). The method is rapid, simple, accurate and reproducible. And the method is proposed to be used for quality control of "Tangzhi Shuangjiang Cha".
Zhang
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GG
Shenyang Pharmaceutical University, Shenyang 110015, China. zhangguogang@hotmail.com
Zeng
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H
Xu
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D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D011786:Quality Control
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C056599:icariin