[Effect of temperature on the activity of some enzymes representative of EMP pathway and TCA cycle in psychrophilic yeast].
Some key enzymes of EMP pathway and TCA cycle in a psychrophilic yeast Y18 were studied in this paper compared with those of Saccharomyces cerevisiae. The results indicated that fructose, 1,6-bisphosphate aldolase, succinate dehydrogenase, and hexokinase in Y18 were very thermolabile and have high activity at low temperature. These enzymes belong to cold-active enzymes. Alpha-ketoglutarate dehydrogenase existed possibly in isoenzyme which had different temperature characteristics. Citrayl synthetase was very similar in temperature characteristics to that of mesophiles. The Km value of succinate dehydrogenase both from Y18 and S. cerevisiae were studied and Some features of enzyme in psychrophiles were also discussed in this paper.
Xin
M
M
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
Zhou
P
P
D003454:Cryptococcus / Q000201:enzymology*; D004795:Enzyme Stability; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013696:Temperature
D007655:Ketoglutarate Dehydrogenase Complex; D013385:Succinate Dehydrogenase; D006593:Hexokinase; D005634:Fructose-Bisphosphate Aldolase