title: Practical asymmetric enzymatic reduction through discovery of a dehydrogenase-compatible biphasic reaction media.
abstract: [reaction: see text] An enzyme-compatible biphasic reaction media for the asymmetric biocatalytic reduction of ketones with in situ cofactor regeneration has been developed. In this biphasic reaction media, which is advantageous for reactions at higher substrate concentrations, both enzymes (alcohol dehydrogenase and FDH from Candida boidinii) remain stable. The reductions with poorly water-soluble ketones were carried out at substrate concentrations of 10-200 mM, and the optically active (S)-alcohols were formed with moderate to good conversions and with up to >99% ee.
authors.affiliation: Degussa AG, Project House Biotechnology, P.O. Box 1345, 63403 Hanau, Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Gröger
authors.forename: Werner
authors.initials: W
authors.lastname: Hummel
authors.forename: Stefan
authors.initials: S
authors.lastname: Buchholz
authors.forename: Karlheinz
authors.initials: K
authors.lastname: Drauz
authors.forename: Tien Van
authors.initials: TV
authors.lastname: Nguyen
authors.forename: Claudia
authors.initials: C
authors.lastname: Rollmann
authors.forename: Hendrik
authors.initials: H
authors.lastname: Hüsken
authors.forename: Kofi
authors.initials: K
authors.lastname: Abokitse
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D004795:Enzyme Stability; D005560:Formate Dehydrogenases / Q000378:metabolism; D006865:Hydrogenation; D007659:Ketones / Q000737:chemistry*; D012997:Solvents
chemical_list: D007659:Ketones; D012997:Solvents; D000426:Alcohol Dehydrogenase; C037212:formate dehydrogenase (NADP+); D005560:Formate Dehydrogenases