Effect of high-oil corn or added corn oil on ruminal biohydrogenation of fatty acids and conjugated linoleic acid formation in beef steers fed finishing diets.
Three Angus steers (410 kg) cannulated in the proximal duodenum were used in a replicated 3 x 3 Latin square to evaluate the effects of dietary lipid level and oil source on ruminal biohydrogenation and conjugated linoleic acid (CLA) outflow. Dietary treatments included: 1) typical corn (TC; 79.2% typical corn), 2) high-oil corn (HOC; 79.2% high-oil corn), and 3) the TC diet with corn oil added to supply an amount of lipid equal to the HOC diet (OIL; 76.9% TC + 2.4% corn oil). Duodenal samples were collected for 4 d following 10-d diet adaptation periods. Data were analyzed with animal, square, period, and treatment in the model and planned, nonorthogonal contrasts were used to test the effects of dietary lipid content (TC vs HOC and OIL) and oil source (HOC vs OIL) on ruminal biohydrogenation. Intake and duodenal flow of total long-chain fatty acids were increased (P < 0.05) by over 63% for diets containing more lipid regardless of oil source. Apparent ruminal dry matter and long chain fatty acid digestibilities were not altered (P > 0.05) by dietary lipid level or oil source. Ruminal biohydrogenation of total and individual 18-carbon unsaturated fatty acids was greater (P < 0.05) for diets with higher lipid content. Biohydrogenation of oleic acid was greater (P < 0.05) for HOC than OIL, but biohydrogenation of linoleic acid was lower (P < 0.05) for HOC than OIL. Duodenal flows of palmitic, stearic, oleic, linoleic, and arachidic acids were more than 30% greater (P < 0.05) for diets containing more lipid. Flow of all trans-octadecenoic acids was greater (P < 0.05) for diets containing more lipid. Corn oil addition increased (P < 0.05) the flow of trans-10 octadecenoic acid and the trans-10, cis-12 isomer of CLA by threefold compared to feeding high-oil corn. Feeding high-oil corn or adding corn oil to typical corn rations increased intake, biohydrogenation, and duodenal flow of unsaturated long-chain fatty acids. Compared with high-oil corn diets, addition of corn oil increased duodenal flow of trans-10, trans-12 and cis-12 isomers of octadecenoic acid and the trans-10, cis-12 isomer of CLA. The amount of cis-9, trans-11 isomer of conjugated linoleic acid flowing to the duodenum was less than 260 mg/d, a value over 20 times lower than flow of trans-11 vaccenic acid indicating the importance of tissue desaturation for enhanced conjugated linoleic acid content of beef.
Duckett
S K
SK
The University of Georgia, Athens, 30602, USA. sduckett@arches.uga.edu
Andrae
J G
JG
Owens
F N
FN
D000818:Animals; D002417:Cattle / Q000378:metabolism*; D003314:Corn Oil / Q000008:administration & dosage* / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism; D004063:Digestion; D004386:Duodenum / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D006865:Hydrogenation; D007536:Isomerism; D019787:Linoleic Acid / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D012417:Rumen / Q000378:metabolism*; D013229:Stearic Acids / Q000378:metabolism
C114874:5-octadecenoic acid; D004041:Dietary Fats; D005227:Fatty Acids; D013229:Stearic Acids; D003314:Corn Oil; D019787:Linoleic Acid