[Effects of BMP on periodontal ligament cell's cell cycling and DNA synthesis: a flow cytometry (FCM) analysis].
OBJECTIVE
To investigate the effects of bone morphogenetic protein (BMP) on the cell cycling and DNA synthesis of periodontal ligament cells (PDLCs).


METHODS
The 7th passage PDLCs were cultured in a 5% CO2, humid atmosphere at 37 degrees C in DMEM supplement with 10% fetal bovine serum (FBS) for 24 hours, then BMP was added in medium with 1% FBS. After being cultured another 72 hours, cells were trypsinized, washed and fixed with cold alcohol for 1 hour. To measure DNA content, 1.0 x 10(6) cells were washed with PBS, resuspended in 200 microliters PBS mixed with staining liquid (RNA ase, 0.1% triton-100 and propidium iodide) for 30 minutes at 4 degrees C. DNA specific fluorescence was measured on a Coulter Epics Profile-II (Coulter, USA) using 250 mW 488 nm laser light for excitating and collecting fluorescence at 610-630 nm. Phase fraction analysis was performed on DNA content histograms with multicycle software.


RESULTS
When compared with the control, the percentage of PDLC in G1 during exponential growth was decreased by a function of BMP, while the percentage of S and (G2 + M) was increased in the experimental group.


CONCLUSIONS
It suggested that the proliferation of PDLC be most probably regulated in the G1 phase of the cell cycle. BMP can be regarded as a positive regulatory growth factor.
Wang
Q
Q
Department of Periodontology, College of Stomatology, Fourth Military Medical University.
Wu
Z
Z
Dong
G
G
Shi
J
J
D000293:Adolescent; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis / Q000096:biosynthesis*; D005434:Flow Cytometry; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects
D019485:Bone Morphogenetic Proteins; D004247:DNA