[Retention behavior of some polycyclic aromatic hydrocarbons on alkylphosphonate-modified magnesia-zirconia composite stationary phase for reversed-phase liquid chromatography].
An alkylphosphonate-modified magnesia-zirconia composite stationary phase (PZMS) for reversed-phase liquid chromatography was prepared. The reversed-phase chromatographic performance of the new packing material was investigated with some polycyclic aromatic hydrocarbons (PAHs) as solutes. The effect of the methanol content in the mobile phase on the retention of PAHs was examined. The logarithmic capacity factor of PAHs was decreased linearly with increasing volume percent of the methanol in the mobile phase, which is similar to that observed on Zorbax ODS. The capacity factor of PAHs on the new stationary phase was also correlated to their partition coefficient between n-octanol and water (log P). A good linear relationship between them was obtained. These results show that the new material can behave as a reversed-phase packing. The separations of phenanthrene and anthracene, and terphenyl isomers were investigated on the new stationary phase and the Zorbax ODS, respectively. The retention of the solutes on the Zorbax ODS is greater than that on the new stationary phase, but the separation selectivity of the solutes on the new stationary phase is better than that on the Zorbax ODS. The better separation selectivity on the new stationary phase may be contributed from the Lewis acid-base interaction between the solutes and the residual Lewis acid sites on the stationary phase.
Fu
H J
HJ
College of Chemistry and Environmental Science, Wuhan University, Wuhan 430072, China.
Feng
Y Q
YQ
Zhang
Q H
QH
Da
S L
SL
Zhang
Y J
YJ
D000478:Alkylation; D000873:Anthracenes / Q000302:isolation & purification; D002614:Chelating Agents; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D008277:Magnesium Oxide; D063065:Organophosphonates / Q000737:chemistry; D010616:Phenanthrenes / Q000302:isolation & purification; D011084:Polycyclic Aromatic Hydrocarbons / Q000302:isolation & purification*; D015040:Zirconium
D000873:Anthracenes; D002614:Chelating Agents; D063065:Organophosphonates; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D008277:Magnesium Oxide; C031181:phenanthrene; D015040:Zirconium; C034020:anthracene; C028541:zirconium oxide