Study on the retention behaviour of metal-tetraphenyl porphine chelates in reversed-phase high performance liquid chromatography.
The retention behaviour of metal chelates of alpha,beta,gamma,delta-tetraphenylporphine (TPP) was studied in reversed-phase high performance liquid chromatography (RP-HPLC). On C18 column, with various organic solvents as mobile phases, the retention of the chelates tends to increase in the following order: ZnTPP < TPP < NiTPP < CuTPP. This retention behaviour can get a good explanation from Horvath's "solvophobic theory". With the assumption that the electrostatic field strength of metal chelates can be measured by the ratio of electronegativity vs. ionic radius (EN/ri) of central metal ion, EN/ri was established to be an index for the retention behaviour of metal-TPP chelates. It was found that lnk' is in good linear relationship with the EN/ri value.
Shi
Z H
ZH
Research Center of Physical and Chemical Analysis, Hebei University, Baoding 071002, China.
Wang
Y H
YH
Fu
C G
CG
D002614:Chelating Agents / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D007502:Iron Chelating Agents / Q000302:isolation & purification; D008665:Metalloporphyrins / Q000302:isolation & purification*; D011166:Porphyrins / Q000302:isolation & purification*
D002614:Chelating Agents; D007502:Iron Chelating Agents; D008665:Metalloporphyrins; D011166:Porphyrins; C076448:zinc tetraphenylporphine; C049692:tetraphenylporphine