[Study on the characteristics and applications of a new hydrophobic interaction chromatographic packing using chitosan as support].
Based on chitosan, by using valeraldehyd as ligand, a new hydrophobic interaction chromatographic (HIC) packing material was prepared by an improved method. The effects of salt concentration, temperature, pH on the adsorption of bovine serum albumin(BSA) were investigated. The results showed that the adsorption behavior of protein by packing material is in agree with the theory of hydrophobic interaction. The alpha-amylase could be separated by HIC with high recovery of enzymatic activity.
Wang
Y
Y
College of Bioscience & Biotechnology, Yangzhou University, Yangzhou 225009, China. wangy1974@263.net
Guo
M L
ML
Jiang
S L
SL
Chen
T
T
Jiang
Y M
YM
Chen
Y
Y
D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D012710:Serum Albumin, Bovine / Q000032:analysis*; D000516:alpha-Amylases / Q000302:isolation & purification
D002686:Chitin; D012710:Serum Albumin, Bovine; D048271:Chitosan; D000516:alpha-Amylases