A simple method for the construction of small format tissue arrays.
Tissue arrays can evaluate molecular targets in high numbers of samples in parallel. Array construction presents technical difficulties and tissue arrayers are expensive, particularly for small and medium sized laboratories. This report describes a method for the construction of 36 sample arrays using widely available materials. A blunted 16 gauge needle for bone marrow aspiration was used to extract paraffin wax cylinders and manually define a 6 x 6 matrix on a blank paraffin wax block. Tissue cores from 36 paraffin wax embedded premalignant lesions and invasive cervical carcinomas were injected into the matrix using a 14 gauge needle. This tissue array was sectioned using a standard microtome and used for the immunodetection of CD44 variant 9 and interleukin 18 with satisfactory results. This method can be applied in any laboratory, without the need of specialised equipment, offering a good alternative for the wider application of tissue arrays.
Hidalgo
A
A
Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Centro Médico Nacional Siglo XXI-IMSS, México DF, 06720.
Piña
P
P
Guerrero
G
G
Lazos
M
M
Salcedo
M
M
D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis; D020382:Interleukin-18 / Q000032:analysis; D008867:Microtomy / Q000379:methods; D016612:Paraffin Embedding / Q000379:methods*; D011230:Precancerous Conditions / Q000175:diagnosis; D013048:Specimen Handling / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis
D014408:Biomarkers, Tumor; D018960:Hyaluronan Receptors; D020382:Interleukin-18