[Direct optical resolution of the enantiomers of biphenyl compounds by high performance liquid chromatography on amylose tris-(3,5-dimethylphenylcarbamate) stationary phase].
A chiral stationary phase(CSP) was prepared by coating amylose tris-(3,5-dimethylphenylcarbamate) onto aminopropylated spherical silica gel. The enantioselective chromatographic separation of a series of biphenyl compounds with antihepatitis activity was performed for the first time on CSP. The effect of alcohol modifiers in mobile phase was investigated. The mobile phases used in the study were hexane modified by ethanol, 1-propanol, 1-butanol or 2-propanol. It was found that the retention times and the separation of the enantiomers of biphenyl compounds on the CSP were dramatically influenced by the nature of alcohols in the mobile phase, and the best alcohol modifier for some compounds was ethanol. The interaction between the stationary phase and samples was also discussed.
Qi
B F
BF
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
Liu
Y Q
YQ
Han
X Q
XQ
Zhu
X Y
XY
Chen
L R
LR
D000688:Amylose / Q000031:analogs & derivatives*; D001713:Biphenyl Compounds / Q000032:analysis / Q000302:isolation & purification*; D002219:Carbamates; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D055095:Optics and Photonics; D048448:Phenylcarbamates; D058428:Silica Gel; D012822:Silicon Dioxide; D013237:Stereoisomerism
D001713:Biphenyl Compounds; D002219:Carbamates; D048448:Phenylcarbamates; C080033:Chiralpak AD; D058428:Silica Gel; D012822:Silicon Dioxide; D000688:Amylose