Thermal stress can inhibit proliferation of ECV304 cells.
OBJECTIVE
To observe the effects of thermal stress on proliferation of human vascular endothelial cells (VECs) and explore its significance.


METHODS
Changes of VECs proliferation were investigated with (3)H-TdR incorporation method after ECV304 was treated at 43 degrees for 2 hours, while expressions of intercellular adhesion molecule-1 (ICAM-1), inhibitor of differentiation-1 (ID1), and P16 and P21 proteins were determined by Western Blotting.


RESULTS
The effect of inhibition of VECs growth after thermal stress was detected by (3)H-TdR incorporation experiment. Western blotting showed ICAM-1, a marker of activated endothelial cells, was increased markedly after thermal stress. Expression of ID1 protein declined gradually with increasing expressions of its downstream genes, P16 and P21 following the thermal stress.


CONCLUSIONS
Thermal stress could strongly activate VECs and inhibit proliferation of VECs through ID1, thus down regulating cyclin-dependent kinase inhibitors, P16 and P21, which might be an essential pathway for recovery of VECs after thermal stress.
Luo
Xiao-feng
XF
Burn Research Institute, Third Military Medical College, Chongqing 400038, China.
Luo
Xiang-dong
XD
Wang
Xian-yuan
XY
D015153:Blotting, Western; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D018257:Helix-Loop-Helix Motifs / Q000502:physiology; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D012097:Repressor Proteins; D013696:Temperature; D014157:Transcription Factors / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology*
C497453:ID1 protein, human; D051798:Inhibitor of Differentiation Protein 1; D012097:Repressor Proteins; D014157:Transcription Factors; D018799:Intercellular Adhesion Molecule-1