[The construction of poliovirus chimera and the analysis of its antigenic structure].
The construction of poliovirus chimera is an useful way to investigate the antigenic structure conformation of poliovirus. In this research, the poliovirus chimera containing a piece of antigenic fragment of hepatitis A virus was constructed for analysis of poliovirus antigenic structure conformation. Depending on the characterization of this chimera, the conformation of poliovirus neutralization antigenic site I in VP1 was analyzed, and that the possible influence of hepatitis A virus antigenic fragment inserted to the structure conformation of poliovirus antigen was also investigated.
Li
Q
Q
Institute of Medical Biology, CAMS, PUMC, Kunming 650107.
Jiang
L
L
Zan
Y
Y
Zhao
H
H
Guo
R
R
D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology*; D002478:Cells, Cultured; D002678:Chimera; D035921:Hepatitis A Antigens / Q000235:genetics; D006801:Humans; D017955:Poliovirus / Q000235:genetics* / Q000276:immunology; D011817:Rabbits; D014162:Transfection
D000956:Antigens, Viral; D035921:Hepatitis A Antigens