[A procedure for developing selective isolation of Pseudonocardiacetes from soil samples].
Pseudonocardiacetes are a group of rare-actinomyces with type IV cell walls and without mycolic acids. Five different selective isolation media S1, S2, S3, S4 and S5 were devised and practiced to improve their selective isolation efficiency. The media were detected by 33 type strains of Pseudonocardiaceae, 4 strains of Saccharothrix and 15 type strains of Streptomyces cultured on them. Soil samples were examined on media S1 ad S2 compared with glucose-asparagine agar. It shows that media S1 and S2 are effective for growth and isolation of Amycolata, Pseudonocardia, Saccharomonospora and other genera of Pseudonocardiaceae.
Lu
Z
Z
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
Qi
W
W
Liu
Z
Z
D000192:Actinomycetales / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D012988:Soil Microbiology
D003470:Culture Media