title: [Chiral separation of enantiomers of synthesized amlodipine and its intermediate by capillary electrophoresis].
abstract: A capillary electrophoretic method for the chiral separation of enantiomers of synthesized amlodipine and its intermediate was developed. Several cyclodextrins (CDs) were applied as the chiral selectors and it was found that the ionic modified carboxymethyl-beta-cyclodextrin (CM-beta-CD) could give satisfactory enantioselectivity. In addition, the effects of the pH value of the buffer system, the concentration of the CD and the voltage on the chiral separation were investigated. The optimized buffer for amlodipine and its intermediate enantiomers was a buffer containing 30 mmol/L phosphate and 50 mmol/L CM-beta-CD (pH 6.12). Under these conditions, the resolutions of enantiomers of amlodipine and its intermediate were 1.73 and 1.55, respectively.
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China.
authors.forename: Bao-hui
authors.initials: BH
authors.lastname: Li
authors.forename: Geng-liang
authors.initials: GL
authors.lastname: Yang
authors.forename: De-xian
authors.initials: DX
authors.lastname: Wang
authors.forename: Zhe-feng
authors.initials: ZF
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
mesh_terms: D017311:Amlodipine / Q000737:chemistry / Q000302:isolation & purification*; D002021:Buffers; D002121:Calcium Channel Blockers / Q000737:chemistry / Q000302:isolation & purification; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
chemical_list: D002021:Buffers; D002121:Calcium Channel Blockers; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C441457:carboxymethyl-beta-cyclodextrin; D017311:Amlodipine