[Influences of porcine enamel matrix proteins on MC3T3-E1 osteoblast proliferation and differentiation].
OBJECTIVE
Some research suggested that enamel matrix proteins(EMPs) not only induced formation of cementum, but also could promote regeneration of periodontal tissue and alveolar bone. In order to recognize the mechanisms of periodontal regeneration induced by EMPs and provide a basic data for further study, influences of EMPs on osteoblast proliferation and differentiation are explored in this study.


METHODS
Porcine enamel matrix proteins were extracted by using acetic acid. MC3T3-E1 osteoblasts were selected and cell culture was used in vitro. EMPs with different concentrations of 50 micrograms/ml, 100 micrograms/ml and 150 micrograms/ml were added separately in alpha-MEM culture medium and compared with negative group and positive group (TGF-beta 1). The count of cell was recorded after 0, 1, 2, 3, 4, 5, 6 days, then the data were analysed statistically by using SAS.


RESULTS
The cell count of all EMPs groups were higher than that in the negative control group (P < 0.01), 100 micrograms/ml as well as 150 micrograms/ml EMPs groups were higher than the positive group(TGF-beta 1) on the second day (P < 0.01). The cell count of 100 micrograms/ml EMPs group was not only higher than C and T groups, but also higher than that of other EMPs dose groups on the fifth day.


CONCLUSIONS
The proliferation and differentiation of osteoblasts can be promoted distinctly by enamel matrix proteins.
Shu
R
R
Department of Oral Medicine, Ninth Affiliated Hospital of Shanghai Second Medical University.
Liu
Z
Z
Ge
L
L
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D013552:Swine
D003746:Dental Enamel Proteins; C109069:enamel matrix proteins