[Electrophoretic migration behavior of deoxyribonucleic acid fragments in three polymer solution concentration regions].
The theory of polymer coils shrinking in semi-dilute solution has recently been developed on polymer solution. The polymer solution from coils shrinking concentration Cs to uniform entangled concentt concentration C+ has been defined as semi-dilute solution. We experimentally investigated the electrophoretic migration behavior of 100 bp deoxyribonucleic acid (DNA) Ladder in hydroxypropyl methyl cellulose (HPMC) concentration ranging from 1.25 g/L to 16.06 g/L. The friction force mobility mu f is used to express the friction force that DNA will encounter in capillary electrophoresis. Our results indicate the division of polymer solution into three regions depending on the relationship of mu f and HPMC concentration and Ferguson plot. Resolutions of 200 bp/300 bp and 700 bp/800 bp show semi-dilute polymer solution suits large fragments and small fragments DNA separation respectively. the results confirm that the current polymer theory is valid under actual CE condition.
Jin
Y
Y
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Lin
B C
BC
Feng
Y S
YS
D001288:Attention; D002021:Buffers; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D065347:Hypromellose Derivatives; D008747:Methylcellulose / Q000031:analogs & derivatives*; D008962:Models, Theoretical; D011108:Polymers; D012996:Solutions
D002021:Buffers; D011108:Polymers; D012996:Solutions; D065347:Hypromellose Derivatives; D008747:Methylcellulose; D004247:DNA