Induction of hemopoiesis by saenghyuldan, a mixture of Ginseng radix, Paeoniae radix alba, and Hominis placenta extracts.
OBJECTIVE
To examine the efficacy of saenghyuldan and its components, Ginseng Radix, Paeoniae Radix Alba, and Hominis Placenta extracts (SHD, GR, PRA, and HP, respectively) on the hemopoiesis in a myelosuppression model system.


METHODS
Susceptibility to cyclophosphamide (CP) and S180 carcinoma was determined in SHD, GR, PRA, and HP-treated mice. Analysis of peripheral blood and bone marrow cells was demonstrated by changes in cell types and histopathologic examination. The expression of cytokine mRNAs involved in hemopoiesis was examined by RT-PCR.


RESULTS
SHD and its separated components (GR, HP, and PRA, respectively) significantly increased the survival in CP- and S180-treated mice. The hematology data demonstrated that all the agents augmented monocyte and leucocyte counts in the peripheral blood and increased bone marrow density and the ratio of leukocyte to erythrocyte in the bone marrow. These findings were positively correlated with the up-regulation of cytokine mRNA expression such as granulocyte colony-stimulating factor (GM-CSF), erythropoietin (EPO), thrombopoietin (TPO), stem cell factor (SCF), and c-Kit.


CONCLUSIONS
SHD is an effective remedy for the bone marrow failure and myelosuppression occurring during chemotherapy.
Son
Chang-Gue
CG
Department of Internal Medicine, College of Oriental Medicine, Daejeon University, 301-724, Korea. ckson@dju.ac.kr
Han
Seung-Hyun
SH
Cho
Jung-Hyo
JH
Shin
Jang-Woo
JW
Cho
Chin-Ho
CH
Lee
Yeon-Weol
YW
Cho
Chong-Kwan
CK
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004338:Drug Combinations; D004921:Erythropoietin / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000235:genetics; D006410:Hematopoiesis / Q000187:drug effects*; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D009368:Neoplasm Transplantation; D029596:Paeonia / Q000737:chemistry; D005894:Panax / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012510:Sarcoma 180 / Q000188:drug therapy / Q000473:pathology; D019089:Stem Cell Factor / Q000096:biosynthesis / Q000235:genetics; D013926:Thrombopoietin / Q000096:biosynthesis / Q000235:genetics
D016207:Cytokines; D004338:Drug Combinations; D008419:Materia Medica; D010936:Plant Extracts; D012333:RNA, Messenger; D019089:Stem Cell Factor; D004921:Erythropoietin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D013926:Thrombopoietin