title: Lower apoptosis rate and higher CD69 expression in neutrophils from atopic individuals.
abstract: OBJECTIVE
  In this study we aimed to examine the kinetics of CD69 expression and the susceptibility to apoptosis, in eosinophils and neutrophils, in the presence or absence of GM-CSF. We also addressed the question whether differences between atopic patients and healthy individuals exist in this respect.
  
  
  METHODS
  Freshly isolated eosinophils and neutrophils from non-atopic and atopic donors were analysed by flow cytometry for Annexin/PI staining, caspase 3 activation and CD69 expression.
  
  
  RESULTS
  We found a higher CD69 expression when atopic neutrophils were incubated with GM-CSF compared to non-atopic neutrophils, and that the kinetics of CD69-expression in neutrophils, but not in eosinophils, differed between non-atopic and atopic individuals (p < 0.004). We also found a higher viability in GM-CSF-stimulated neutrophils from non-atopic individuals as compared to neutrophils from atopic individuals (p < 0.05).
  
  
  CONCLUSIONS
  These data suggest a potential role for neutrophils in the allergic inflammatory reaction through differences in apoptosis rates and CD69 expression between atopic and non-atopic individuals.
authors.affiliation: Department of Medicine, Karolinska Hospital/Institute, Stockholm, Sweden. Anna.Nopp@ks.se
authors.forename: A
authors.initials: A
authors.lastname: Nopp
authors.forename: H
authors.initials: H
authors.lastname: Stridh
authors.forename: R
authors.initials: R
authors.lastname: Grönneberg
authors.forename: J
authors.initials: J
authors.lastname: Lundahl
mesh_terms: D017302:Annexins / Q000378:metabolism; D015703:Antigens, CD / Q000096:biosynthesis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis*; D017209:Apoptosis / Q000502:physiology*; D020169:Caspases / Q000378:metabolism; D002469:Cell Separation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004804:Eosinophils / Q000473:pathology / Q000502:physiology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D016179:Granulocyte Colony-Stimulating Factor / Q000502:physiology; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000378:metabolism* / Q000473:pathology*; D066298:In Vitro Techniques; D037181:Lectins, C-Type; D009336:Necrosis; D009504:Neutrophils / Q000473:pathology / Q000502:physiology*; D000099192:CD69 Antigens
chemical_list: D017302:Annexins; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D020169:Caspases; D016179:Granulocyte Colony-Stimulating Factor; D037181:Lectins, C-Type; D000099192:CD69 Antigens