cDNA cloning and characterization of the third isoform of human peroxin Pex11p.
We have cloned a human cDNA encoding an isoform of the peroxin Pex11p, termed Pex11pgamma, by BLASTP homology search on eukaryotic protein database and reverse transcription (RT)-PCR of human fibroblast RNA. Pex11pgamma was 241 amino-acid long, with two putative transmembrane segments, showing 22% and 23% amino-acid identity to Pex11palpha and Pex11pbeta, respectively. PEX11gamma gene was located on chromosome 19, distinct from PEX11alpha and PEX11beta. Pex11pgamma was found to be a peroxisomal membrane protein, as assessed by colocalization with peroxisome targeting signal type 1 (PTS1)-proteins, in epitope-tagged Pex11pgamma-expressing Chinese hamster ovary cells. Pex11pgamma exposes both of the N- and C-terminal parts to the cytosol. PEX11gamma was not induced in rats by treatment of clofibrate, a peroxisome proliferator, similar to constitutively expressed PEX11beta but in contrast to inducible PEX11alpha.
Tanaka
Atsushi
A
Department of Biology, Faculty of Sciences, Kyushu University Graduate School, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Okumoto
Kanji
K
Fujiki
Yukio
Y
D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002994:Clofibrate / Q000494:pharmacology; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary; D006801:Humans; D000960:Hypolipidemic Agents / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000502:physiology; D008565:Membrane Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D000074401:Peroxins; D020033:Protein Isoforms / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism; D051381:Rats; D016415:Sequence Alignment
D018076:DNA, Complementary; D000960:Hypolipidemic Agents; D008565:Membrane Proteins; C490455:PEX11A protein, human; C490453:PEX11B protein, human; D000074401:Peroxins; C490454:Pex11a protein, rat; D020033:Protein Isoforms; D002994:Clofibrate