title: Tissue-specific ICAM-1 expression and neutrophil transmigration in the copper-deficient rat.
abstract: Dietary copper deficiency promotes neutrophil accumulation in rat lungs. We have now investigated the potential mechanisms of this effect. Male weanling rats were fed a Cu-adequate (6.0 mg diet) or Cu-deficient diet (0.30 mg) for 4 wks. Endothelial intercellular adhesion molecule-1 (ICAM-1) expression was measured in vivo and in vitro using a radiolabeled monoclonal antibody to rat ICAM-1. Tissue neutrophil accumulation was measured by myeloperoxidase (MPO) content and neutrophil transendothelial migration was assessed in vitro. Dietary copper deficiency had no effects on the expression of ICAM-1 in lung, liver, heart, kidney, or cremaster. However, MPO content was significantly greater in the lungs of copper-deficient rats. Endotoxin-induced ICAM-1 expression was greater in the lungs and hearts of copper-deficient rats. Similarly, cultured rat endothelial cells that were Cu-chelated expressed more ICAM-1 after endotoxin. This correlated with the significant increase in MPO in lungs of copper-deficient rats treated with endotoxin. The results suggest a tissue-specific difference in ICAM-1 expression and neutrophil accumulation during inflammation in copper-deficient rats. The findings suggest that lung inflammatory mechanisms are particularly sensitive to copper deficiency.
authors.affiliation: Department of Physiology and Biophysics, University of Louisville, Louisville, KY 40292, USA. daschu01@louisville.edu
authors.forename: Dale A
authors.initials: DA
authors.lastname: Schuschke
authors.forename: Susan S
authors.initials: SS
authors.lastname: Percival
authors.forename: David
authors.initials: D
authors.lastname: Lominadze
authors.forename: Jack T
authors.initials: JT
authors.lastname: Saari
authors.forename: Alex B
authors.initials: AB
authors.lastname: Lentsch
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology; D003300:Copper / Q000172:deficiency*; D005786:Gene Expression Regulation / Q000502:physiology; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D008297:Male; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018799:Intercellular Adhesion Molecule-1; D003300:Copper