[Study of the acupuncture effect on monoamine transmitters in rabbit plasma and brain tissue by high performance liquid chromatography with electrochemical detection].
A rapid and simple method for the study of the acupuncture effect on monoamine transmitters and related compounds in rabbit plasma and brain tissue by high performance liquid chromatography with electrochemical detection was developed. An ODS column was selected as the separation column at 25 degrees C, and pH 4.50, 0.02 mol/L of trisodium citrate-0.05 mol/L sodium phosphate dibasic to methanol (95:5, volume ratio) without ion-pair at a flow rate of 1.0 mL/min. Four compounds, epinephrine (E), norepinephrine (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT), were simultaneously separated and determined under the above conditions. Twenty rabbits were investigated after the acupuncture action upon the central neurotransmitters. The sufficient data showed that acupuncture could significantly affect the activities of the neurotransmitters including E, NE, DA and 5-HT, and the changed functions of the neurotransmitter systems induced by acupuncture not only lead to the neurotransmitter content increase both in brain and plasma but also cause the increase of rabbit breed ability. The results show that the method is very simple and fast. The method is valuable not only for clinical diagnosis but also for research work.
Hou
Jing-Guo
JG
Department of Chemistry, Northwest Normal University, Lanzhou 730070, China. houjg@nwnu.edu.cn
Liu
Hui-Ling
HL
He
Tian-Xi
TX
Wang
Zhu-Ming
ZM
Mao
Xue-Feng
XF
Du
Xin-Zhen
XZ
Deng
Hua-Ling
HL
Gao
Jin-Zhang
JZ
D000818:Animals; D015306:Biogenic Monoamines / Q000097:blood / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004298:Dopamine / Q000378:metabolism; D015671:Electroacupuncture; D004563:Electrochemistry; D004837:Epinephrine / Q000378:metabolism; D005260:Female; D009638:Norepinephrine / Q000378:metabolism; D011817:Rabbits; D012701:Serotonin / Q000378:metabolism
D015306:Biogenic Monoamines; D012701:Serotonin; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine