The effect of Erigeron breviscapus on proliferation of pulmonary artery smooth muscle cells in hypoxic porcines.
In order to study the effect of Erigeron Breviscapus (EB) on proliferation of pulmonary artery smooth muscle cells (PASMC) in hypoxic porcines, immunohistochemical and MTT methods were employed to measure the proliferation of PASMC. It was found that the proliferation of PASMC in porcines was obvious, and the expression of proliferating cell nuclear antigen (PCNA) was significantly high within 48 h after exposure to hypoxia. The EB could inhibit the proliferation and the expression of PCNA in PASMC under hypoxia, but it had no effect on the proliferation and expression of PCNA in PASMC under normal condition. The EB could inhibit the proliferation and the expression of PCNA in PASMC induced by phorbol 12-myristate 13-acetate (PMA), an agonist of PKC in normal and hypoxic conditions. It was concluded that the hypoxia could enhance the proliferation and expression of PCNA in PASMC. The EB can inhibit the proliferation and expression of PCNA in PASMC under hypoxia through PKC-signal way. The EB may be used in treating the pulmonary hypertension by inhibiting the proliferation of PASMC and the pulmonary vascular remodeling.
Ding
Y
Y
Department of Respiratory Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Xu
Y
Y
Zhang
Z
Z
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D015687:Cell Hypoxia; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D011651:Pulmonary Artery / Q000166:cytology; D011753:Pyrones / Q000494:pharmacology*; D013552:Swine
D004365:Drugs, Chinese Herbal; D011753:Pyrones; C030416:erigeroside