[Capillary array electrophoresis and high-throughput DNA sequencing].
Depending on the outputs of 10,080 DNA sequencing samples from the human genome project, technical features of capillary array electrophoresis are discussed. The results show that 88% of the total readlength were higher than 50 kbp/run. It means that most of the readlength could reach 500 bp or more with reasonable quality. The results were much better than expected when the technology of capillary array appeared about one year ago. A key parameter affecting the quality of DNA sequencing is the purity of sequencing template. Therefore, a batch agarose slab electrophoresis was applied to check the purity before loading. Other conditions, such as concentration of template and loading amount, are discussed as well. Finally, comparisons based on the electrophoresis timing, the pre-treatment of samples, the loading procedures and the results analysis between capillary array electrophoresis and slab gel electrophoresis have been made. From the point of view of high-throughput DNA sequencing, especially in the field of genome research, the capillary array electrophoresis should be the better choice as a more efficient technical platform.
Zhen
Z C
ZC
Chinese National Human Genome Center, Beijing 100176, China.
Yao
Z J
ZJ
D001483:Base Sequence; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D019075:Electrophoresis, Capillary / Q000379:methods*; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*