title: Erythropoietin prevents ischemia-reperfusion from inducing oxidative damage in fetal rat brain.
abstract: OBJECTIVE
  The aim of this study was to show the effect of erythropoietin on ischemia-reperfusion-induced oxidative damage in fetal rat brain.
  
  
  METHODS
  Fetal brain ischemia was induced by clamping the utero-ovarian artery bilaterally for 20 min, and reperfusion was achieved by removing the clamps for 30 min. The control group was made up of non-injured rats that were 19 days pregnant. In the ischemia-reperfusion group no treatment was given, while 0.4 ml of human serum albumin solution and 5,000 U/kg recombinant human erythropoietin (r-Hu-EPO) were administered in the vehicle and treatment groups 30 min before ischemia-reperfusion injury. Lipid peroxidation in the brain tissue was determined as the concentration of thiobarbituric acid-reactive substances (TBARS) for each fetal rat. A one-way analysis of variance and the post-hoc test were used for statistical analysis.
  
  
  RESULTS
  TBARS increased to statistically significantly higher levels in fetal rat brain after ischemia-reperfusion injury than were found in the control group. Recombinant human erythropoietin prevented the increase in TBARS after ischemia-reperfusion injury.
  
  
  CONCLUSIONS
  Recombinant human erythropoietin has been shown to have neuroprotective effect in intrauterine ischemia-reperfusion-induced fetal brain damage in rats.
authors.affiliation: Department of Neurosurgery, Ankara Numune Research and Educational Hospital, Ankara, Turkey.
authors.forename: Ihsan
authors.initials: I
authors.lastname: Solaroglu
authors.forename: Ayse
authors.initials: A
authors.lastname: Solaroglu
authors.forename: Erkan
authors.initials: E
authors.lastname: Kaptanoglu
authors.forename: Suat
authors.initials: S
authors.lastname: Dede
authors.forename: Ali
authors.initials: A
authors.lastname: Haberal
authors.forename: Etem
authors.initials: E
authors.lastname: Beskonakli
authors.forename: Kamer
authors.initials: K
authors.lastname: Kilinc
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism*; D004921:Erythropoietin / Q000494:pharmacology*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D010084:Oxidation-Reduction; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*
chemical_list: D011994:Recombinant Proteins; D004921:Erythropoietin