[Melanin properties studies of the recombinant of Pseudomonas pseudoalcaligenes containing tyrosinase gene].
Pseudomonas pseudoalcaligenes is a notably killing maggots bacterium, which was isolated from natural dead maggots. Tyrosinase gene of P. maltophilia AT18 has been introduced into P. pseudoalcaligenes, and enabled it the ability of producing melanin steadily. Antiradiation effect of the melanin is quite strong. The melanin of the recombinant is nonfixiform material. It's solubility is very little in many sorts of organic or inorganic solvent. It's solubility is big in alkaline solution, but little in neutral or weak acid solution. It is deposited when pH < 4. It is oxidized by H2O2 or NaC10. It is also reduced by Ag+ or H2S. It has free radical and it can absorbs free radical generated by ultra-violent ray. It can absorbs ray of all sorts of wave length. The absorption rate of ultra-violet ray is the biggest in all sorts of wave length. It can effectively protects protein, DNA and other biomacromolecule matter against the damages by ultra-violet ray.
Cai
X
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Department of Biology, Yancheng Normal College, Yancheng 224002, China.
D016166:Free Radical Scavengers / Q000494:pharmacology; D005798:Genes, Bacterial; D008543:Melanins / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D014442:Monophenol Monooxygenase / Q000235:genetics* / Q000378:metabolism; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D044205:Pseudomonas pseudoalcaligenes / Q000235:genetics* / Q000378:metabolism; D011835:Radiation Protection; D011994:Recombinant Proteins / Q000235:genetics; D012995:Solubility; D014169:Transformation, Bacterial; D014466:Ultraviolet Rays
D016166:Free Radical Scavengers; D008543:Melanins; D011994:Recombinant Proteins; D014442:Monophenol Monooxygenase