title: [The effect of heat shock before rat partial hepatectomy on HSC70/HSP68 expression and phosphatase activities].
abstract: The contribution and content of the continuous heat shock protein 70/induced heat shock protein 68 (HSC70/HSP68), the contribution, variety and activity of acid phosphatases (ACP) and alkaline phosphatases (AKP) had been analysed qualitatively and quantitatively during the liver regeneration after 2/3 hepatectomy (PH) and HS (heat shock at 46 degrees C for 30 min, recovery for 8 h), which were compared with the results only by HS and only by PH. It was shown that the three kinds of treatment all can increase the activity of ACP, AKP and the expression of HSC70/HSP68, but with different change pattern. A further analysis show that after HS-PH the enhanced activity of ACP is related with that of 140 kD phosphatases, the enhanced activity of AKP is associated with that of 140 kD and 160-180 kD phosphatases. It can be reckoned from the results that ACP, AKP and HSC70/HSP68 all act on the heat shock response of hepatocyte and liver regeneration, and may take part in signal transduction in these processes, but ACP may play a dominant role in the start of hepatocyte multiplication, AKP and HSC70/HSP68 may play a dominant role in cytokineses.
authors.affiliation: College of Life Sciences, Henan Normal University, Xinxiang, 453002.
authors.forename: C S
authors.initials: CS
authors.lastname: Xu
authors.forename: A L
authors.initials: AL
authors.lastname: Lu
authors.forename: M
authors.initials: M
authors.lastname: Xia
authors.forename: X Y
authors.initials: XY
authors.lastname: Li
authors.forename: Y H
authors.initials: YH
authors.lastname: Li
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhao
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D005260:Female; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D018869:Heat-Shock Response / Q000502:physiology*; D006498:Hepatectomy; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000502:physiology
chemical_list: D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C097210:Hspa1a protein, rat; C494373:Hspa8 protein, rat; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase