RFLP analysis of 1-aminocyclopropane-1-carboxylate synthase ACC2 and ACC4 genes from Polish cultivars of tomato.
An important trait of tomato is the rate of fruit ripening, strongly dependent on ethylene production. The ripening-related ethylene synthesis in tomato is controlled mainly by 1-aminocyclopropane-1-carboxylate synthase LE-ACS2 and LE-ACS4 isoenzymes (Rottmann et al., 1991, J. Mol. Biol. 222: 937; Lincoln et al., 1993, J. Biol. Chem. 268: 19422; Barry et al., 2000, Plant Physiol. 123: 979). In spite of numerous reports on the LE-ACS2 and LE-ACS4 gene expression, only ones considered the genomic organisation each of these genes (Rottmann et al., 1991; Lincoln et al., 1993) reported one copy of each of these genes in tomato cv VF36. In this article we suggest that the genomic organisation of LE-ACS2 and LE-ACSS4 genes may depend on tomato cultivars and may differ from that described by the above authors. The results of Southern analyses of genomic DNAs from 17-day old seedlings (cultivars Jaga, Halicz, Betalux, New Yorker) imply that the genomic organisation of LE-ACS2 and LE-ACS4 genes in Polish cultivars differs from that reported for cv VF36.
Jakubowicz
Małgorzata
M
Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Miedzychodzka 5, 60-371 Poznań, Poland. goja@amu.edu.pl
Jółkowska
Justyna
J
Kwapiszewska
Grazyna
G
Krecisz
Anna
A
Czerski
Piotr
P
D015139:Blotting, Southern; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D008190:Lyases / Q000235:genetics*; D018551:Solanum lycopersicum / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D036226:Seedlings
D018076:DNA, Complementary; D008190:Lyases; C021773:1-aminocyclopropanecarboxylate synthase