[Determination of ferulic acid in chuanxiong and in animal serum and cerebrospinal fluid by reversed-phase high performance liquid chromatography].
An easy, rapid and sensitive method for the determination of ferulic acid(FA) in Chuanxiong extracts, animal (mouse) serum and cerebrospinal fluid by RP-HPLC has been developed. The FA was separated on an ODS column, Nova-Pak C18(3.9 mm i.d. x 150 mm) and detected at the wavelength of 320 nm. The mobile phase was methanol-water-acetic acid (35:65:0.5, V/V), with a flow rate of 0.8 mL/min. The detection limit of FA was 1.7 micrograms/L(S/N = 3) and the calibration curve was linear within the range of 0.85 mg/L-4.00 mg/L(r = 0.99904, n = 6). The mean recovery from animal serum and cerebrospinal was 95%-102%.
Lü
K
K
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Ding
M Y
MY
Li
H X
HX
Liu
D L
DL
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003373:Coumaric Acids / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005260:Female; D030004:Ligusticum; D008297:Male; D008516:Medicine, Chinese Traditional; D051381:Rats; D017208:Rats, Wistar; D014665:Vasodilator Agents / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid
D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D014665:Vasodilator Agents; C004999:ferulic acid; C101574:chuangxiong extract