Induction of syngeneic intradermal and orthotopic epidermal carcinomas in hairless Skh-1 mice as a reproducible model for experiments.
Epidermoid carcinomas, clinically and histologically similar to human squamous cell carcinomas (SCC), were obtained in hairless Skh-1 mice. Tumor cells originated from chemically-induced skin cancers. We developed three models of orthotopic skin tumors: (1) intradermal injection of a tumor cell suspension, (2) superficial abrasion of the skin, cell grafting and application of a hydrocolloid dressing, (3) skin incision, seeding and application of a hydrocolloid dressing. Intradermal injection was 100% successful. Skin incision, displaying histological evidence of rapid invasive tumor growth, was 75% successful. Though skin tumor growth after abrasion was only 20% successful, the tumor histogenesis exactly imitated human SCC development. These carcinomas provide research models for further experiments such as photodynamic therapy or antiangiogenesis therapy.
Christophe
Marianne
M
Faculté des Sciences Pharmaceutiques et Biologiques, Université Henri Poincaré-Nancy I, Unité de Recherche en Thérapie Photodynamique du Centre Alexis Vautrin, Vandoeuvre-lès-Nancy, France. marianne.christophe@free.fr
Bisson
Jean-François
JF
Notter
Dominique
D
Parache
Robert Michel
RM
Vigneron
Claude
C
Guillemin
François
F
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D007271:Injections, Intradermal; D051379:Mice; D008812:Mice, Hairless; D009368:Neoplasm Transplantation / Q000379:methods*; D015203:Reproducibility of Results; D012878:Skin Neoplasms / Q000139:chemically induced / Q000473:pathology*