title: [The influence of somatostatin 14 on the proliferation of human retroocular fibroblasts].
abstract: OBJECTIVE
  To elucidate the possible mechanism of Somatostatin 14 in the treatment of Graves' ophthalmopathy, thus providing laboratory data with the intent to underpin therapeutic developments.
  
  
  METHODS
  The human retro-ocular fibroblasts (HROFb) was cultured and MTT colorimetry assay was used to determine the proliferation of HROFb.
  
  
  RESULTS
  MTT colorimetry assay could precisely reflect the proliferation of HROFb. The absorbance was directly proportional to the numbers of the cells (r = 0.996, P < 0.001). Somatostatin 14 could enhance the cell proliferation percentage at lower levels (< 25 nmol/L) and inhibit that at higher levels (> 25 nmol/L). The cell proliferation correlated positively to Somatostatin 14 levels significantly (r = -0.9054, P < 0.001).
  
  
  CONCLUSIONS
  Somatostatin 14 has a dual effect on HROFb.
authors.affiliation: Division of Endorcrinology, First Affiliated Hospital, WCUMS, Chengdu 610041, China.
authors.forename: Z
authors.initials: Z
authors.lastname: An
authors.forename: S
authors.initials: S
authors.lastname: Wei
authors.forename: S
authors.initials: S
authors.lastname: Xu
authors.forename: H
authors.initials: H
authors.lastname: Yang
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003124:Colorimetry / Q000379:methods; D005123:Eye / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology*; D006111:Graves Disease / Q000473:pathology; D006801:Humans; D013004:Somatostatin / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D013778:Tetrazolium Salts; D013844:Thiazoles; D013004:Somatostatin; C022616:thiazolyl blue