Novel method for the immobilization of nucleotides.
[reaction: see text] A novel method for the immobilization of nucleotides has been developed. The strategy employs a highly reactive pyrrolidinium phosphoramidate zwitterion intermediate that undergoes nucleophilic attack by long-chain alkylamine-controlled pore glass (LCAA-CPG) to generate an immobilized nucleotide. Quantification of nucleotide loading was accomplished by acidic hydrolysis of the P-N bond and subsequent HPLC analysis of TMP in the presence of an internal standard. Typical nucleotide loadings of 51-59 micromol/g of support were observed.
Freel Meyers
Caren L
CL
Department of Medicinal Chemistry and Molecular Pharmacology and Cancer Center, Purdue University, West Lafayette, Indiana 47907, USA. caren_meyers@hms.harvard.edu
Borch
Richard F
RF
D000577:Amides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D009711:Nucleotides / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010756:Phosphoric Acids / Q000737:chemistry
D000577:Amides; D009711:Nucleotides; D010756:Phosphoric Acids; C011067:phosphoramidic acid