[Recombinant Salmonella typhimurium anticaries vaccine. II. Construction and confirmation of expressing plasmid carrying a region of S. mutans pac gene].
OBJECTIVE
The objective of this study is to construct prokaryotic expressing plasmids carrying A region of S. mutans pac gene.


METHODS
The A region of pac gene was amplified by using PCR from plasmid pPC41 containing S. mutans surface protein pac gene. The new expression plasmid was constructed by using A region of pac gene and expressing plasmids pET17-b and directed cloning technique. The recombinant plasmid was identified by agarose gel electrophoresis after it was cut by using BamHI and EcoRV.


RESULTS
The expressing plasmid carrying pac gene A region was acquired.


CONCLUSIONS
The recombinant plasmid provides useful experimental materials for further research work.
Chen
H
H
College of Stomatology, Sun Yat-sun University of Medical Sciences.
Ling
J
J
Yang
G
G
Tu
J
J
D003731:Dental Caries / Q000517:prevention & control*; D015870:Gene Expression; D005798:Genes, Bacterial; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic; D022562:Salmonella Vaccines / Q000235:genetics* / Q000276:immunology; D012486:Salmonella typhimurium / Q000235:genetics* / Q000276:immunology; D013295:Streptococcus mutans / Q000235:genetics*
D022562:Salmonella Vaccines