[Preliminary studies on the apoptosis of Spodoptera litura cells induced by Two baculoviruses].
Microscopic examination of Spodoptera litura(SL-1) cell infected with AcMNPV or SfaMNPV revealed progressive cell blebbing starting at 8 h-12 h postinfection and culminating in total cell destruction at 24 h postinfection. The fragmentation of the infected cell nuclei was observed by stained with the specific fluorescent dye DAPI. Agarose gel electrophoresis analysis of the DNA extracted from infected cells show typical DNA ladder. All these supported that both viruses infected SL-1 cells undergo apoptosis. Through lipofectin transinfection, we observed that only the DNA of AcMNPV or SfaMNPV can also induce apopotosis of SL-1 cell. Virus titer analysis revealed that neither viruses can duplicate in SL-1 cells.
Yao
Lunguang
L
Institute of Entomology, Central China Normal University, Wuhan 430079, China.
Du
Changsheng
C
Peng
Jianxin
J
Hong
Huazhu
H
D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics; D017924:Nucleopolyhedroviruses / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D018411:Spodoptera / Q000166:cytology* / Q000821:virology; D014162:Transfection; D014779:Virus Replication
D004279:DNA, Viral