[The murine submandibular gland conditioned medium induce the development of CFU-GM and CFU-meg].
OBJECTIVE
Our purpose is to determine whether murine submandibular gland produce hematopoietic growth factors or not and which hematopoietic growth factor are produced by murine submandibular gland.


METHODS
By using the techniques of culture of hematopoietic progenitor cells(CFU-GM, CFU-Meg) ex vivo, flow-cytometry, the effects of murine submandibular gland conditioned medium(SGCM) on the development of CFU-GM and CFU-Meg in mice was studied.


RESULTS
SGCM can stimulate the development of CFU-GM and CFU-Meg (without any exogeneous HGF), almost no colony in control (without any exogeneous HGF and SGCM); the promoting activity of male SGCM is higher than female SGCM's in augmenting colony formation of CFU-Meg (P < 0.05), there is no difference of CFU-GM(P > 0.05). The stimulating activity of anemic mice SGCM is higher normal mice SGCM's (P < 0.05). IL-3 can collaborate with female SGCM to stimulate proliferation of CFU-Meg. SGCM can promote bone morrow cell entiring the active proliferative phase(S/G2).


CONCLUSIONS
Submandibular gland can stimulate the development of CFU-GM and CFU-Meg by secreting hematopoietic growth factor-like activities. Our study provide the experimental evidences for clarifying the relationship between submandibular gland and hematopoiesis.
Pan
K
K
Department of Biology and Cell Biology, School of Basic Medical Science, WCUMS, Chengdu 610041, China.
Zhu
B
B
Wu
Y
Y
D000818:Animals; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D017077:Culture Media, Conditioned; D046508:Culture Techniques; D058851:GPI-Linked Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D006410:Hematopoiesis; D016298:Hematopoietic Cell Growth Factors / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000166:cytology; D008297:Male; D008562:Membrane Glycoproteins; D000090204:Mesothelin; D051379:Mice; D008807:Mice, Inbred BALB C; D014176:Protein Biosynthesis; D011506:Proteins; D013363:Submandibular Gland / Q000378:metabolism*
D017077:Culture Media, Conditioned; D058851:GPI-Linked Proteins; D016298:Hematopoietic Cell Growth Factors; D008562:Membrane Glycoproteins; D011506:Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D000090204:Mesothelin