[Cloning and prokaryotic expression of viral binding protein(VBP) gene from endosybiotic bacterium of Rhopalosiphum padi].
Viral binding protein gene from Rhopalosiphum padi Yangling biotype was amplified by PCR method and then cloned. The complete nucleotide sequence of the gene was determined, It has 1647 nucleotides encoding 548 amino acids. Comparison showed this gene has 97% identity on nucleotide level with Buchnera groEL-AM gene of Rhopalosiphum padi American biotype, while has 97.4% identity on amino acid level was found between this two genes. The VBP gene was ligated into pBV221 and pET30a expression vector and expressed the aim protein 63 kD and 69 kD.
Wu
Yunfeng
Y
Northwest Scie-Tech University of Agriculture and Forestry, Yangling, 712100, China.
Cui
Xiaofeng
X
Lin
Lin
L
Zhou
Guanghe
G
D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000737:chemistry*; D001483:Base Sequence; D018834:Chaperonin 60 / Q000235:genetics*; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D017385:Sequence Homology; D013559:Symbiosis
D018834:Chaperonin 60; D004268:DNA-Binding Proteins