Response of larval Xenopus laevis to atrazine: assessment of growth, metamorphosis, and gonadal and laryngeal morphology.
Larval Xenopus laevis were exposed to one of four concentrations of atrazine (0, 1, 10, or 25 microg/L, 11 replicate tanks per treatment, 60-65 larvae per replicate) dissolved in an artificial pond water (frog embryo teratogenesis assay- Xenopus [FETAX]) medium beginning 48 h after hatching until the completion of metamorphosis. Separate groups of larvae (six replicate tanks per treatment, 60-65 larvae per replicate) were exposed to estradiol (100 microg/L), dihydrotestosterone (100 microg/L), or ethanol vehicle control dissolved in FETAX medium. None of the treatments affected posthatch mortality, larval growth, or metamorphosis. There were no treatment effects on sex ratios except for estradiol, which produced a greater percentage of female offspring. Exposure to either estradiol or 25 microg atrazine/L increased the incidence of intersex animals based on assessment of gonadal morphology. Atrazine did not reduce the size of the laryngeal dilator muscle, a sexually dimorphic muscle in this species. We conclude that environmentally relevant concentrations of atrazine do not influence metamorphosis or sex ratios and do not inhibit sexually dimorphic larynx growth in X. laevis. The incidence of atrazine-induced intersex animals was small (<5%) and occurred only at the greatest concentration of atrazine tested, a concentration that is rarely observed in surface waters in the United States.
Carr
James A
JA
Department of Biological Sciences, Texas Tech University, Lubbock, Texas 79409, USA. james.carr@ttu.edu
Gentles
Angie
A
Smith
Ernest E
EE
Goleman
Wanda L
WL
Urquidi
Lina J
LJ
Thuett
Kerry
K
Kendall
Ronald J
RJ
Giesy
John P
JP
Gross
Tim S
TS
Solomon
Keith R
KR
Van Der Kraak
Glen
G
D000818:Animals; D001280:Atrazine / Q000633:toxicity*; D013196:Dihydrotestosterone / Q000633:toxicity; D012734:Disorders of Sex Development / Q000139:chemically induced; D004958:Estradiol / Q000633:toxicity; D005260:Female; D006066:Gonads / Q000187:drug effects / Q000254:growth & development*; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D007830:Larynx / Q000187:drug effects / Q000254:growth & development*; D008297:Male; D008675:Metamorphosis, Biological / Q000187:drug effects*; D009132:Muscles / Q000187:drug effects; D012744:Sex Ratio; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D014982:Xenopus laevis
D014874:Water Pollutants, Chemical; D013196:Dihydrotestosterone; D004958:Estradiol; D001280:Atrazine