Imaging of ultra-weak bio-chemiluminescence and singlet oxygen generation in germinating soybean in response to wounding.
Ultra-weak bio-chemiluminescence (UBC) from germinating soybean (Glycine max L. Merr) cotyledon under mechanical wounding was observed using a high-sensitivity imaging system based on an ICCD detector and a highly sensitive single photon counter (SPC) device. The UBC imaging showed that the intensity at the injury location on a wounded cotyledon was obviously enhanced as compared with that at the non-injured point. The UBC intensity of wounded cotyledons was initially very high and reached a stationary state after about 5 min. Wounding-induced emission could be suppressed by wounding in the presence of sodium azide. Deuterium oxide amplified the emission intensity. It was concluded that singlet oxygen ((1)O(2)) was the main cause of the emission during the wounding phase.
Chen
Wen Li
WL
Institute of Laser Life Science, South China Normal University, Guangzhou 510631, People's Republic of China.
Xing
Da
D
Tan
ShiCi
S
Tang
YongHong
Y
He
Yonghong
Y
D018548:Cotyledon / Q000187:drug effects / Q000502:physiology; D018525:Germination / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D049449:Luminescence; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D012680:Sensitivity and Specificity; D026082:Singlet Oxygen / Q000032:analysis / Q000378:metabolism*; D019810:Sodium Azide / Q000494:pharmacology; D013025:Glycine max / Q000187:drug effects / Q000502:physiology*
D017382:Reactive Oxygen Species; D026082:Singlet Oxygen; D019810:Sodium Azide