[The effect of fibrin sealant on dental pulp for pulp capping in experimental dogs].
OBJECTIVE
To investigate the pulp tissue reaction and reparative dentine formation of dog pulp capped with Fibrin Sealant (FS).


METHODS
The fibrin was from a tisseel Kit (Immuno AG, Vienna, Austria) consisting of solution A (freeze-dried human fibrinogen, factor XIII, Aprotinine) and solution B (thrombin powder, calcium chloride) with 0.02 ml from each for each tooth. The Ca(OH)2 paste (Calar, China) was served as the control. Forty nine cuspid, premolar and molar teeth in 4 adult dogs weighed 11-13 kg were used. Surgical anesthesia was obtained by muscle injection of 3% sodium pentobarbital (1 ml/kg). The pulp were exposed by using sterile high-speed rotary cutting instruments with water-cooling. The pulps were treated with 0.02 ml fibrin or Ca(OH)2 paste respectively. The cavity was filled with zine phosphate cement. The animals were killed at intervals of 1, 4, 6, 9 weeks after surgery and perfused with phosphate-buffered saline followed by 10% formalin in phosphate-buffered saline. The jaws were resected and the samples prepared by removing each experimental tooth in block, further fixing them by immersion in 10% formalin in phosphate-buffered saline. The specimen were processed for histologic examination. Serial sections (5 nm thick) were made, stained with hematoxylin and eosin, examined using a light microscope.


RESULTS
7 days after operation, the samples treated with the FS showed no hemorrhage and degeneration of pulp tissue and a small amount inflammatory cells near the wounds. The samples treated with Ca(OH)2 showed hemorrhage and degeneration (2/6) and a large amount inflammatory cells near the wounds. 28 days after operation, pulp health of teeth treated with FS and Ca(OH)2 was nearly recovered. Dentin bridges were observed in 2 out of 6 cases treated with Ca(OH)2. 42 days after operation, pulp health of teeth treated with FS and Ca(OH)2 was totally recovered. Dentin bridges were observed in 2 out of 7 cases of pulp treated with FS and four out of 6 cases treated with Ca(OH)2. 63 days after operation, pulp health of teeth treated with FS and Ca(OH)2 was soundly resumed. The dentine bridge was observed in 4 out 6 cases of pulp treated with the FS and in all 6 cases treated with Ca(OH)2.


CONCLUSIONS
FS has no dentin inducing activity, but can enhance exposed pulp healing, so FS can be served as a promised pulp capping agent.
Ren
W
W
College of Stomatology, Fourth Military Medical University.
Yang
L
L
Chen
X
X
Li
Y
Y
D000818:Animals; D003782:Dental Pulp / Q000187:drug effects* / Q000473:pathology; D003785:Dental Pulp Capping; D003804:Dentin / Q000187:drug effects* / Q000473:pathology; D004285:Dogs; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*
D015718:Fibrin Tissue Adhesive