[Molecular cloning and amino acid composition analysis of a halophilic thiolase gene].
5' and 3' end sequence of acaBl gene as primers, the gene of halophilic thiolase from haloarchae, Halobacterium sp. ZP-6 was cloned and its amino acid composition was calculated. Compared with non-halophilic thiolase, the halophilic thiolase contains more negative charge amino acid, less positive amino acid and less strong hydrophobic amino acid, and use preferably small side-chain amino acid. Those suggest that electrostatic screen, hydrophobic effect and surface tension all contribute to halophilic properties of thiolase.
Liu
Tiehan
T
Institute of Microbiology, Chinese Academy of Science, Beijing 100080, China.
Zhou
Peijin
P
D000101:Acetyl-CoA C-Acetyltransferase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis* / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D006217:Halobacterium / Q000201:enzymology* / Q000235:genetics; D019617:Halobacterium salinarum / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D000596:Amino Acids; D000101:Acetyl-CoA C-Acetyltransferase