[Preparation and evaluation of phosphatidylcholine coated on silica as the biomembrane stationary phase].
The method of reverse phase evaporation is used to coat phosphatidylcholine(PC) directly on the surface of silica, which is used as biomembrane chromatographic solid phase to study the interactions between drugs and biomembrane. It was observed that the solid phase coated phosphatidylcholine had a good stability at 20 degrees C-30 degrees C, and the stability would be improved by the presence of appropriate amount of cholesterol in phosphatidylcholine. The content of cholesterol in phosphatidylcholine, the nature of buffer, the concentration of salt in buffer and the pH of mobile phase could all affect chromatographic retention of drugs on the prepared biomembrane column. Six compounds, polyethylene glycol, mannitol, salicylic acid, warfarin, hydrocortisone, and cortisone have been tested. The biomembrane chromatographic stationary phase coated PC with silica as matrices can be simply prepared and it is possible to simulate the human's physiological environment by the biomembrane chromatographic system, so it is a useful method to study drug absorption and distribution in human body.
Mao
X Q
XQ
National Chromatography R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China.
Zou
H F
HF
Luo
Q Z
QZ
Kong
L
L
Li
X
X
Sun
N C
NC
D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D006854:Hydrocortisone / Q000032:analysis; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D012822:Silicon Dioxide; D014859:Warfarin / Q000032:analysis*
D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D014859:Warfarin; D012822:Silicon Dioxide; D006854:Hydrocortisone