Detection of the parasitic dinoflagellate Hematodinium in the Norway lobster Nephrops norvegicus by ELISA.
Norway lobsters Nephrops norvegicus from the coastal waters of Scotland are seasonally infected by a parasitic dinoflagellate of the genus Hematodinium. An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of the parasite in the haemolymph of N. norvegicus. The ELISA is simple to perform with a detection limit of 5 x 10(4) parasites ml(-1) haemolymph. The ELISA is currently being used to study the prevalence and seasonality of Hematodinium infection in N. norvegicus and other crustacean hosts.
Small
H J
HJ
Division of Environmental and Evolutionary Biology, Graham Kerr Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK. 9909258s@student.gla.ac.uk
Wilson
S
S
Neil
D M
DM
Hagan
P
P
Coombs
G H
GH
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D015153:Blotting, Western / Q000662:veterinary; D004141:Dinoflagellida / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D006458:Hemolymph / Q000276:immunology; D008121:Nephropidae / Q000469:parasitology*; D015995:Prevalence; D012606:Scotland / Q000453:epidemiology; D012621:Seasons; D012680:Sensitivity and Specificity
D000913:Antibodies, Protozoan