Expression of a glycogen synthase protein kinase homolog from Colletotrichum gloeosporioides f.sp. malvae during infection of Malva pusilla.
The potential role of a GSK3 protein kinase homolog, cggsk, was examined from Colletotrichum gloeosporioides f.sp. malvae, a fungal pathogen of Malva pusilla. A peak in cggsk expression relative to a constitutively expressed fungal actin gene occurred during host penetration and was followed by much lower expression levels during subsequent biotrophic and necrotrophic growth in host tissue. The peak level of cggsk expression observed during penetration was 21-fold greater than that during necrotrophic growth. Expression of cggsk showed small but reproducible changes during growth in culture; however, the levels were always similar to that during necrotrophic growth in the host. One possible role for cggsk could be to coordinate fungal development during host penetration.
Goodwin
Paul H
PH
Department of Environmental Biology, University of Guelph, Guelph, ON N1G 2W1, Canada. pgoodwin@uoguelph.ca
Chen
Grace Y J
GY
D000595:Amino Acid Sequence; D015139:Blotting, Southern; D020231:Colletotrichum / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D028501:Malva / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D017385:Sequence Homology; D013997:Time Factors
D038362:Glycogen Synthase Kinase 3