A genetic interval and physical contig spanning the Peronospora parasitica (At) avirulence gene locus ATR1Nd.
In Peronospora parasitica (At) (downy mildew), the genetic determinants of cultivar-specific recognition by Arabidopsis thaliana are the Arabidopsis thaliana-recognised (ATR) avirulence genes. We describe the identification of 10 amplified fragment length polymorphism (AFLP) markers that define a genetic mapping interval for the ATR1Nd avirulence allele, the presence of which is perceived by the RPP1Nd resistance gene. Furthermore, we have constructed a P. parasitica (At) bacterial artificial chromosome (BAC) library comprising over 630Mb of cloned DNA. We have isolated 16 overlapping clones from the BAC library that form a contig spanning the genetic interval. BAC sequence-derived markers and a total mapping population of 311 F(2) individuals were used to refine the ATR1Nd locus to a 1cM interval that is represented by four BAC clones and spans less than 250kb of DNA. This work demonstrates that map-based cloning techniques are feasible in this organism and provides the critical foundations for cloning ATR1Nd using such a strategy.
Rehmany
Anne P
AP
Horticulture Research International, Wellesbourne, Warwick CV35 9EF, UK. anne.rehmany@hri.ac.uk
Grenville
Laura J
LJ
Gunn
Nick D
ND
Allen
Rebecca L
RL
Paniwnyk
Zennia
Z
Byrne
Jane
J
Whisson
Stephen C
SC
Birch
Paul R J
PR
Beynon
Jim L
JL
D000483:Alleles; D017360:Arabidopsis / Q000235:genetics / Q000382:microbiology*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D020451:Contig Mapping; D004247:DNA / Q000235:genetics*; D005819:Genetic Markers; D015698:Genomic Library; D009868:Oomycetes / Q000302:isolation & purification / Q000472:pathogenicity; D020161:Physical Chromosome Mapping; D010838:Phytophthora / Q000235:genetics* / Q000472:pathogenicity
D005819:Genetic Markers; D004247:DNA