[Identification and location of the toxin protein genes in 56 Bacillus thuringiensis isolates].
Polymerase chain reaction was performed to screen Bacillus thuringiensis isolates for cryI, cryIII, and cryV genes with cryI-, cryIII-, and cryV-specific primers respectively. The results indicated that, in the 56 Bt isolates examined, 7 isolates contain cryI gene, 2 isolates contain cryIII gene, and 21 isolates contain both cryI and cryV genes. The cryV gene was located by Southern hybridization in the large plasmid of about 150 MD molecular mass.
Zhong
X
X
Institute of Entomology and State Key Laboratory for Biocontrol, Zhongshan University, Guangzhou 510275, China.
Yuan
M
M
Zhang
P
P
Deng
R
R
Long
Q
Q
Pang
Y
Y
Wang
X
X
D001413:Bacillus thuringiensis / Q000737:chemistry / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D001427:Bacterial Toxins / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004731:Endotoxins / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D010957:Plasmids; D016133:Polymerase Chain Reaction
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D017931:DNA Primers; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis