[Study on quantitative relationship between retention parameters of solutes in reversed-phase liquid chromatography].
A quantitative relationship between lg I (a constant relating to the affinity of one mole of solute to stationary phase) and Z (total moles of organic solvent released at the contact region as one mole solute adsorbed by stationary phase) in the stoichiometric displacement model for retention (SDM-R) of solute was compared with the corresponding relationship in Snyder's Equation in reversed-phase liquid chromatography (RPLC). It was found that the relationship from SDM-R was more exact than that from Snyder's Equation. The linearity depends on the ranges of both lg I and lg Kw0. The broader the range, the better the linearity is. This conclusion conflicts to that reported in references in which the linearity only exists either when one factor dominates solute retention, or when it is completely dominated by statistics. The linear relationship in SDM-R and in Snyder's Equation was found valid not only for homologues but also for non-homologues.
Guo
H
H
Shaanxi Provincial Key Laboratory of Modern Separation Science, Institute of Modern Separation Science, Northwest University, Xi'an 710069, China.
Zhang
Y J
YJ
Gao
J
J
Geng
X D
XD
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016014:Linear Models; D046911:Macromolecular Substances; D008433:Mathematics
D046911:Macromolecular Substances