[Study on the forensic application of ABO genotyping by PCR-RFLP].
OBJECTIVE
The study was carried out on the application of ABO genotyping by PCR-RFLP to forensic purpose.


METHODS
The DNA of samples were extracted by Chelex--100 and phenol/chloroform, the PCR products were separated by Native Horizontal PAGE, and detected by silver staining.


RESULTS
The PCR amplified fragment length of ABO loci is from 175 to 210 bp, the frequency distribution of six genotypes ranges from 0.0250 to 0.4300, heterozygosity(H) and discriminating power(Dp) is 0.5162 and 0.7111.


CONCLUSIONS
This technique has a good application to the forensic samples such as blood, blood stain, semen stain, hair root, bone tissue and semen from vaginal swabs.
Wang
X L
XL
Forensic Science Institute of Zhongshan City, Zhongshan 528403.
Cheng
X Z
XZ
Liu
C
C
Liu
C
C
Yang
W
W
Zhang
J F
JF
D000017:ABO Blood-Group System / Q000235:genetics*; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D000017:ABO Blood-Group System