New HLA-DRB1*07 allele identified from microlymphocytotoxicity/SSP discrepancies.
A HLA-DRB1*07 variant allele has been identified in a cadaver kidney donor. Serological typing using monoclonal antibodies detected HLA-DR4 and HLA-DR7. HLA class II DNA typing using sequence-specific primer (PCR-SSP) polymerase chain reaction only detected DRB1*04, while sequence-specific oligonucleotide (PCR-SSO) polymerase chain reaction confirmed the presence of both DRB1*04 and DRB1*07 alleles, although two extra reactions were also found. Exon 2 of the HLA-DRB1*07 was isolated using allele-specific PCR, then cloned and sequenced. Four mutations, at positions 170 (T --> C), 171 (C --> T), 174 (C --> G), and 179 (C --> A), were observed. These mutations changed codons 57 and 60 (V --> A; S --> Y, respectively). This amino acid sequence at position 56-61 is only found in DRB1*0811.
Donvito
B
B
Laboratoire d'Immunologie UFR Médicine, Physio-Pathologie Dysimmunitaire Humaine (PPDH), Cedex, France.
Perrier
P
P
Tonye Libyh
M
M
Tabary
T
T
Philbert
F
F
Reveil
B
B
Cohen
J H M
JH
D000483:Alleles; D001483:Base Sequence; D003601:Cytotoxicity Tests, Immunologic; D005091:Exons; D006684:HLA-DR Antigens / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D059811:HLA-DRB1 Chains; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains