Human c-fos promoter mediates high-level, inducible expression in various mammalian cell lines.
The promoter activity of the human c-fos and human cytomegalovirus (CMV) immediate early promoter was compared in transient and stable transfection experiments with six cell lines of mouse, human, and hamster origin which are all of commercial importance. The c-fos promoter was 1.8-5.6-fold stronger than the CMV promoter in BHK-A, BHK-B, CHO-DHFR(-), and mouse NIH-3T3 in stable transfectants and less effective in mouse myeloma or human 293 cells, suggesting a new transcriptional control element for high-level expression and protein production in mammalian cells. The induction profiles determined in the presence and absence of serum are dependent on the cell line used. Induction levels of up to 8-fold could be achieved in preselected cell pools.
Bi
Jing-Xiu
JX
Biochemical Engineering Division, GBF - German Research Centre for Biotechnology, Braunschweig, D-38124 Germany.
Buhr
Petra
P
Zeng
An-Ping
AP
Wirth
Manfred
M
D016475:3T3 Cells / Q000166:cytology / Q000378:metabolism; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D016466:CHO Cells / Q000166:cytology / Q000378:metabolism; D002460:Cell Line; D006224:Cricetinae / Q000235:genetics; D003587:Cytomegalovirus / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D016762:Genes, fos / Q000235:genetics*; D049452:Green Fluorescent Proteins; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D007668:Kidney / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D008164:Luminescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D013045:Species Specificity; D014162:Transfection / Q000379:methods*
D000956:Antigens, Viral; D017874:Immediate-Early Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C044582:immediate-early proteins, cytomegalovirus; D049452:Green Fluorescent Proteins