[Simple preparation and evaluations of amide-octyl-bonded phase].
A simple preparation process of amide-octyl-bonded phase (AOBP) is described. In the process aminopropyltriethoxysilane first reacted with octanoyl chloride and then the obtained intermediate was bonded onto porous silica. Characterization of prepared packing was carried out with elemental analysis, solid-state nuclear magnetic resonance (13C-NMR) and Fourier transform infrared (FTIR) spectroscopy. Chromatographic evaluations were carried out by testing with a mixture including acidic, basic and neutral organic compounds using methanol-water as binary mobile phase. The results show that the stationary phase has excellent chromatographic properties and resistance to hydrolysis between pH 2.5-7.5. It can be used for the efficient separation of basic compounds.
Huang
Xiao-jia
XJ
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, China.
Wang
Jun-de
JD
Liu
Xue-liang
XL
Cong
Run-zi
RZ
D020003:1-Octanol / Q000737:chemistry; D000577:Amides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D010208:Papaverine / Q000302:isolation & purification; D010546:Perphenazine / Q000302:isolation & purification; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide; D017550:Spectroscopy, Fourier Transform Infrared
D000577:Amides; D011437:Propylamines; D012821:Silanes; C088294:3-aminopropyltrimethoxysilane; D012822:Silicon Dioxide; D010208:Papaverine; D010546:Perphenazine; D020003:1-Octanol