title: Simultaneous determination of the monoamine neurotransmitters and glucose in rat brain by microdialysis sampling coupled with liquid chromatography-dual electrochemical detector.
abstract: A new type of chemically modified electrode based ring-disk electrode as the dual electrochemical detector (DECD) for high-performance liquid chromatography (HPLC) to simultaneously determine the monoamine neurotransmitters and glucose is described. The ring electrode was modified with an ion-exchange polymer-overoxidized polypyrrole (OPPy) and the disk electrode was modified with nano Au colloid and glucose oxidase (GOD). The electrochemical behaviors of dopamine (DA) and ascorbic acid (AA) at the OPPy chemically modified electrode (CME) were investigated by differential pulse voltammetry (DPV). It was found that the CME could permeate dopamine cations and repelled the ascorbate anions, which could be used to determine the monoamine neurotransmitters and avoid the interference of AA. The electrochemical behavior of glucose at the Nafion/GOD-Au colloid/GC CME was investigated by amperometry and flow injection analysis (FIA). It was found that the sensitivity of the CME increased apparently in determination of glucose. In order to obtain better separation and current responses of the analytes in HPLC-DECD, several operational parameters have been investigated. Under the optimum conditions, the method showed good stability and reproducibility. The application of this method coupled with microdialysis sampling for in vivo simultaneous determination of monoamine neurotransmitters and glucose in rat brain was satisfactory.
authors.affiliation: Department of Chemistry, East China Normal University, Shanghai 200062, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Xuni
authors.initials: X
authors.lastname: Cao
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Xie
authors.forename: Shiyun
authors.initials: S
authors.lastname: Ai
authors.forename: Litong
authors.initials: L
authors.lastname: Jin
authors.forename: Jiye
authors.initials: J
authors.lastname: Jin
mesh_terms: D000818:Animals; D015306:Biogenic Monoamines / Q000032:analysis*; D001923:Brain Chemistry; D002853:Chromatography, Liquid; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized; D017022:Flow Injection Analysis; D005465:Fluorocarbon Polymers; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D017979:Gold Colloid; D006863:Hydrogen-Ion Concentration; D017551:Microdialysis; D017072:Neostriatum / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D051381:Rats; D015203:Reproducibility of Results
chemical_list: D015306:Biogenic Monoamines; D004800:Enzymes, Immobilized; D005465:Fluorocarbon Polymers; D017979:Gold Colloid; D011758:Pyrroles; C040402:perfluorosulfonic acid; D005949:Glucose Oxidase; D005947:Glucose