Immunodiagnosis of bovine fasciolosis using Fasciola hepatica excretory-secretory antigens ELISA.
Two hundreds blood samples and the corresponding faecal and/or bile (114 Egyptian buffaloes, 68 native breed cattle and 18 imported cattle) were examined for Fasciola infection. ELISA revealed that 8 (7.00%) buffaloes, 52 (76.50%) native breed cattle and 15 (83.33%) imported cattle were positive. Parasitologically, the results revealed that 38 (33.33%) buffaloes and 18 (26.50%) native breed cattle were positive, while all imported cattle were free from Fasciola. So, ELISA technique using F. hepatica excretory-secretory (E/S) antigens is of diagnostic value for cattle fasciolosis rather than for buffaloes.
el-Aziz
M M
MM
Department of Parasitology and Animal Diseases, National Research Center, Dokki, Giza, Egypt.
Ghazy
A A
AA
Effat
M M
MM
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology; D001646:Bile / Q000469:parasitology; D002020:Buffaloes / Q000276:immunology / Q000469:parasitology; D002417:Cattle / Q000469:parasitology; D002418:Cattle Diseases / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000175:diagnosis* / Q000276:immunology / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D010270:Parasite Egg Count
D000947:Antigens, Helminth