[Prokaryotic expressed trichosanthin and other two proteins have anti-fungal activity in vitro].
DNAs encoding Trichosanthin, tobacco class I Chitinase and tobacco class I beta-1, 3-Glucanase were expressed in E. coli, respectively. The expression products were assayed for their anti-fungal activity. All of three kinds of proteins show anti-fungal activity. When two of them combined, this activity was enhanced greatly. When three of them combined, the stronger anti-fungal activity was observed.
Hu
P
P
National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, 100871.
An
C
C
Li
Y
Y
Chen
Z
Z
D000935:Antifungal Agents / Q000494:pharmacology*; D002688:Chitinases / Q000096:biosynthesis / Q000494:pharmacology*; D004357:Drug Synergism; D004926:Escherichia coli / Q000235:genetics; D005658:Fungi / Q000187:drug effects*; D043326:Glucan 1,3-beta-Glucosidase / Q000096:biosynthesis / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D014026:Nicotiana / Q000201:enzymology; D015978:Trichosanthin / Q000096:biosynthesis / Q000494:pharmacology*
D000935:Antifungal Agents; D011994:Recombinant Proteins; D015978:Trichosanthin; D002688:Chitinases; D043326:Glucan 1,3-beta-Glucosidase