Urinary concentrations of morphine after the administration of herbal teas containing Papaveris fructus in relation to doping analysis.
A quantitative method for the analysis of morphine in human urine in the concentration range between 0.25 and 2 microg/ml is described and validated. Morphine was determined after enzymatic hydrolysis of the urine. After liquid-liquid extraction with dichloromethane-methanol (9:1) at pH 9.5, morphine was derivatized with N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) and analyzed with GC-MS (full scan). The limit of quantification of the method was 0.25 microg/ml. Two Papaveris fructus containing herbal teas were administered to five male volunteers and urine samples were taken quantitatively during the first 12 h after the administration. The morphine concentration in the tea was 10.4 and 31.5 microg/ml, respectively. Morphine was detected in the urine of all volunteers by 1 h after drinking the tea. Maximum morphine concentrations, 4.3 and 7.4 microg/ml, respectively, were obtained 4-6 h after administration. Doping positive urine samples were delivered for 1-9 h.
Van Thuyne
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Doping Control Unit, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Van Eenoo
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Delbeke
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D000328:Adult; D000701:Analgesics, Opioid / Q000032:analysis / Q000652:urine*; D001628:Beverages / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D009020:Morphine / Q000032:analysis / Q000652:urine*; D010207:Papaver / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D015813:Substance Abuse Detection
D000701:Analgesics, Opioid; D007202:Indicators and Reagents; D009020:Morphine