Degradation of benzyldimethylalkylammonium chloride by Aeromonas hydrophila sp. K.
OBJECTIVE
The aim of this work was to study the biodegradation of benzyldimethylalkylammonium chloride (BAC) by Aeromonas hydrophila sp. K, an organism isolated from polluted soil and capable of utilizing BAC as sole source of carbon and energy.


RESULTS
High performance liquid chromatography and gas chromatography-mass spectrometry (GC-MS) analysis was used to study BAC degradation pathway. It was shown that during BAC biodegradation, formation of benzyldimethylamine, benzylmethylamine, benzylamine, benzaldehyde and benzoic acid occurred. Formation of benzyldimethylamine as the initial metabolite suggested that the cleavage of Calkyl-N bond occurred as the first step of BAC catabolism. Liberation of benzylmethylamine and benzylamine likely resulted from subsequent demethylation reactions, followed by deamination with formation of benzaldehyde. Benzaldehyde was rapidly converted into benzoic acid, which was further degraded.


CONCLUSIONS
Aer. hydrophila sp. K is able to degrade BAC. A degradation pathway for BAC and related compounds is proposed.


CONCLUSIONS
These findings are significant for understanding biodegradation pathways of benzyl-containing quaternary ammonium compounds.
Patrauchan
M A
MA
Department of Microbiology and Immunology, University of British Columbia, Michigan State University, USA. marianna@interchange.ubc.ca
Oriel
P J
PJ
D016980:Aeromonas hydrophila / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D001547:Benzaldehydes / Q000378:metabolism; D019817:Benzoic Acid / Q000378:metabolism; D001593:Benzyl Compounds / Q000378:metabolism; D001596:Benzylamines / Q000378:metabolism; D001673:Biodegradation, Environmental; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003470:Culture Media; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D012988:Soil Microbiology
D001547:Benzaldehydes; D001593:Benzyl Compounds; D001596:Benzylamines; D003470:Culture Media; D019817:Benzoic Acid; C030796:benzylamine; C032175:benzaldehyde; C045966:N-benzyl-N,N-dimethylamine