[Studies on preparation of L-phenylalanine from phenylpyruvic acid by E. coli EP8-10].
E. coli EP8-10 was selected from the soil. It was able to produce the transaminase with high activity when it was cultivated on the medium containing peptone and beef extract. Optimum conditions of enzyme reaction was: phenylpyruvic acid's concentration of 0.3-0.5 mol/L, L-Asptaric acid used as amino donor, pH 8.5 37 degrees C. When phenylpyruvic acid was 0.3 mol/L, 48 g/L L-phenylalanine was produced after 6 h with 97% conversion rate.
Xu
H
H
Department of Biotechnology and Bioengineering, Nanjing Institute of Chemical Technology, Nanjing 210009.
Ouyang
P
P
Zhou
W
W
D003470:Culture Media; D004926:Escherichia coli / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism*; D010667:Phenylpyruvic Acids / Q000378:metabolism*; D000637:Transaminases / Q000378:metabolism
D003470:Culture Media; D010667:Phenylpyruvic Acids; D010649:Phenylalanine; D000637:Transaminases