Differential calcium response in HeLa and HeLa-Fas cells by cytotoxic T lymphocytes.
We constructed a CD95 overexpressing HeLa cell line which was extremely sensitive towards CD95 mediated apoptosis. In these CD95 overexpressing cells, CD95 blocks the nuclear calcium signal induced by perforin positive and CD95 ligand positive killer cells. This phenomenon is highly relevant in states of inflammatory syndromes such as systemic inflammatory response syndrome (SIRS) and sepsis which are associated with a high probability to reactivate latent viruses due to a functional deficiency of cytotoxic effectors.
Schneider
E Marion
EM
Sektion Experimentelle Anästhesiologie, Universitätsklinikum Ulm, Steinhövelstr. 9, Ulm D-89075, Germany. marion.schneider@medizin.uni-ulm.de
Menzl
Ina
I
Weber
Olaf
O
Hug
Hubert
H
D017209:Apoptosis / Q000502:physiology*; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism; D003601:Cytotoxicity Tests, Immunologic; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D008024:Ligands; D008562:Membrane Glycoproteins / Q000378:metabolism; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000378:metabolism*; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism*
D005456:Fluorescent Dyes; D007376:Interleukin-2; D008024:Ligands; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D019014:fas Receptor; D054353:Perforin; D002118:Calcium