[Production and some properties of a beta-glucanase from Trichoderma sp. GXC].
The factors affecting Trichoderma sp. GXC for beta-glucanase (1,3-1,4-beta-D-glucan 4-glucanohydrolase, EC 3.2.1.73) production have been investigated, the optimal conditions were that carbon and nitrogen sources were bran and ammonium sulfate, respectively, initial pH 4.0-5.0, shaken at 30 degrees C for 44 h. beta-glucanase was purified to electrophoretic homogeneity by using ammonium sulfate precipitation, Sephadex G-100 and DEAE-Sephadex A-50 chromatography. It's molecular weight was 35 kD by SDS-PAGE, and the enzyme functioned optimally at pH6.0 and 50 degrees C, respectively. In addition, the enzyme was stable at pH 4.0-5.0 and below 40 degrees C. The beta-glucanase activity was significantly inhibited by Fe3+ and stimulated by Co2+, respectively.
Sun
J
J
Institute of Feed Science, College of Animal Science, Zhejiang University, Hangzhou 310029, China.
Li
W
W
Gu
S
S
D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D014242:Trichoderma / Q000737:chemistry / Q000201:enzymology*
D005656:Fungal Proteins; D006026:Glycoside Hydrolases; C028020:licheninase