title: Cutting edge: profile of chemokine receptor expression on human plasma cells accounts for their efficient recruitment to target tissues.
abstract: We systematically examined the repertoire of chemokine receptors expressed by human plasma cells. Fresh bone marrow plasma cells and myeloma cells consistently expressed CXCR4, CXCR6, CCR10, and CCR3. Accordingly, plasma cells responded to their respective ligands in chemotaxis and very late Ag-4-dependent cell adhesion to fibronectin. Immobilized CXC chemokine ligand (CXCL)16, a novel transmembrane-type chemokine and CXCR6 ligand, also directly induced adhesion of plasma cells without requiring G(alpha i) signaling or divalent cations. Furthermore, we revealed consistent expression of CXCL12 (CXCR4 ligand), CXCL16 (CXCR6 ligand), and CC chemokine ligand 28 (CCR10 and CCR3 ligand) in tissues enriched with plasma cells including bone marrow, and constitutive expression of CXCL12, CXCL16, and CC chemokine ligand 28 by cultured human bone marrow stromal cells. Collectively, plasma cells are likely to be recruited to bone marrow and other target tissues via CXCR4, CXCR6, CCR10, and CCR3. CXCR6 may also contribute to tissue localization of plasma cells through its direct binding to membrane-anchored CXCL16.
authors.affiliation: Department of Microbiology, Kinki University School of Medicine, Osaka-Sayama, Osaka, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakayama
authors.forename: Kunio
authors.initials: K
authors.lastname: Hieshima
authors.forename: Dai
authors.initials: D
authors.lastname: Izawa
authors.forename: Youichi
authors.initials: Y
authors.lastname: Tatsumi
authors.forename: Akihisa
authors.initials: A
authors.lastname: Kanamaru
authors.forename: Osamu
authors.initials: O
authors.lastname: Yoshie
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism; D002448:Cell Adhesion / Q000276:immunology; D002465:Cell Movement / Q000276:immunology*; D002478:Cells, Cultured; D054413:Chemokine CCL11; D054377:Chemokine CXCL12; D054382:Chemokine CXCL13; D000075743:Chemokine CXCL16; D018925:Chemokines / Q000096:biosynthesis; D019742:Chemokines, CC / Q000378:metabolism / Q000502:physiology; D019743:Chemokines, CXC / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology; D006801:Humans; D008024:Ligands; D008565:Membrane Proteins / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology; D009101:Multiple Myeloma / Q000276:immunology; D009928:Organ Specificity / Q000276:immunology; D010950:Plasma Cells / Q000276:immunology* / Q000378:metabolism* / Q000502:physiology; D054447:Receptors, CCR10; D054397:Receptors, CCR3; D019718:Receptors, CXCR4 / Q000096:biosynthesis / Q000378:metabolism; D000075744:Receptors, CXCR6; D019707:Receptors, Chemokine / Q000096:biosynthesis* / Q000378:metabolism; D018121:Receptors, Cytokine / Q000096:biosynthesis / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D011971:Receptors, Immunologic; D051116:Receptors, Scavenger; D011991:Receptors, Virus; D017154:Stromal Cells / Q000276:immunology / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: C515456:CCL11 protein, human; C414047:CCL28 protein, human; C515706:CCR10 protein, human; C515350:CCR3 protein, human; C515255:CXCL12 protein, human; C110663:CXCL13 protein, human; C423727:CXCL16 protein, human; C106330:CXCR6 protein, human; D054413:Chemokine CCL11; D054377:Chemokine CXCL12; D054382:Chemokine CXCL13; D000075743:Chemokine CXCL16; D018925:Chemokines; D019742:Chemokines, CC; D019743:Chemokines, CXC; D008024:Ligands; D008565:Membrane Proteins; D054447:Receptors, CCR10; D054397:Receptors, CCR3; D019718:Receptors, CXCR4; D000075744:Receptors, CXCR6; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D043562:Receptors, G-Protein-Coupled; D011971:Receptors, Immunologic; D051116:Receptors, Scavenger; D011991:Receptors, Virus