[Culture and characterization of human peritoneal mesothelial cells].
OBJECTIVE
To establish a reproducible model for culture of human peritoneal mesothelial cells (HPMCs), and observe the expressions of extracellular matrix (ECM) protein and interleukin-8 (IL-8) in HPMC.


METHODS
Mesothelial cells were isolated from human omenta by trypsin EDTA disaggregation. HPMCs were identified by morphology and streptomyces protein-peroxidase (SP) method. In the same way, expressions of fibronectin, collagen type III, V and transforming growth factor beta 1 (TGF beta 1) were measured. Expression of IL-8 mRNA and FN mRNA in the HPMCs was detected by reverse transcription (RT) and polymerase chain reaction (PCR) amplification.


RESULTS
Confluent HPMCs appeared multipolar under microscope; numerous surface microvilli and an abundant endoplasmic reticulum were observed. Cultured HPMCs coexpressed cytokeratin and vimentin, and synthesized fibronectin, TGF beta 1 and collagen types III (instead of type V). Expressions of IL-8 mRNA and FN mRNA were also observed in HPMCs.


CONCLUSIONS
Establishment of cultured HPMCs model will provide the basis of the research in preventment of peritoneal fibrosis and roles of IL-8 in peritoneal dialysis.
Liu
F Y
FY
Department of Nephrology, Second Xiangya Hospital, Central South University, Chiangsha 410011, China.
Duan
S B
SB
Long
Z G
ZG
D015415:Biomarkers; D002478:Cells, Cultured; D024061:Collagen Type III / Q000096:biosynthesis / Q000235:genetics; D004848:Epithelium; D005353:Fibronectins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D009852:Omentum / Q000166:cytology*; D010530:Peritoneal Dialysis; D010537:Peritoneum / Q000166:cytology; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics
D015415:Biomarkers; D024061:Collagen Type III; D005353:Fibronectins; D016209:Interleukin-8; D016212:Transforming Growth Factor beta