title: Determination of corticosterone in mouse plasma by a sweeping technique using micellar electrokinetic chromatography.
abstract: The separation and on-line concentration of corticosterone in mouse blood was achieved by means of capillary electrophoresis/UV absorbance using sodium dodecyl sulfate (SDS) as a surfactant. The procedure involved the use of an on-line sample concentration method by sweeping-micellar electrokinetic chromatography (sweeping-MEKC). Optimal on-line concentration and separation conditions were determined. The detection limit for this method was 5 ng/ml (S/N=3) and photodiode array detection at 247 nm was used for identification. For the analysis of actual samples, corticosterones from blood samples of a non-stressed and stressed mouse were determined. The results show that only a minor amount of corticosterone was produced by a non-stressed mouse, whereas a significant amount was present in the blood sample from a stressed mouse. The method developed here can be used to examine corticosterone levels as a marker of stress in test animals and may also be used for estimating the effect of stress-release medications.
authors.affiliation: Department of Chemistry, National Taiwan Normal University, 88 Sec 4, Tingchow Road, Taipei, Taiwan.
authors.forename: Chun-Hsueh
authors.initials: CH
authors.lastname: Wu
authors.forename: Mei-Chuan
authors.initials: MC
authors.lastname: Chen
authors.forename: An-Kai
authors.initials: AK
authors.lastname: Su
authors.forename: Pin-Yan
authors.initials: PY
authors.lastname: Shu
authors.forename: Shiu-Huey
authors.initials: SH
authors.lastname: Chou
authors.forename: Cheng-Huang
authors.initials: CH
authors.lastname: Lin
mesh_terms: D015069:18-Hydroxycorticosterone / Q000097:blood; D000818:Animals; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary; D003345:Corticosterone / Q000097:blood*; D007202:Indicators and Reagents; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009862:Online Systems; D012967:Sodium Dodecyl Sulfate; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents
chemical_list: D002021:Buffers; D007202:Indicators and Reagents; D012997:Solvents; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D015069:18-Hydroxycorticosterone; D003345:Corticosterone