Monoclonal antibodies against 3-deoxy-alpha-D-manno-oct-2-ulosonic acid (Kdo) and D-glycero-alpha-D-talo-oct-2-ulosonic acid (Ko).
Monoclonal antibodies (mAbs) were obtained after immunization of mice with neoglycoconjugates containing as a ligand the disaccharide Kdo(2-->4)Ko or Ko(2-->4)Kdo, representing structural elements of the core region of lipopolysaccharides (LPSs) from Acinetobacter haemolyticus and Burkholderia cepacia, respectively. One antibody, S67-9, bound with high specificity to Ko(2-->4)Kdo-BSA showing no cross reactivity with Kdo(2-->4)Kdo-BSA and the other antigens tested. A second mAb, S68-12, bound preferentially to Kdo(2-->4)Ko-BSA but cross reacted with Ko(2-->4)Kdo-BSA and Kdo(2-->4)Kdo-BSA. A third mAb, S67-27, was found to bind Kdo monosaccharide. Although mAbs S67-9 and S68-12 did not bind to LPS of Burkholderia or Acinetobacter as expected, the mAbs will be useful tools in studying the biosynthesis of LPS containing Ko.
Brade
Lore
L
Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany.
Gronow
Sabine
S
Wimmer
Norbert
N
Kosma
Paul
P
Brade
Helmut
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D016956:Burkholderia cepacia / Q000737:chemistry; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009844:Oligosaccharides / Q000737:chemistry* / Q000276:immunology*
D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides; D009844:Oligosaccharides