Comparison of the TUNEL, lamin B and annexin V methods for the detection of apoptosis by flow cytometry.
The present study was performed in order to compare the sensitivity of 3 apoptosis-detection methods using a model cell culture after induction of apoptosis. Evaluation was performed by means of flow cytometry and light microscopy. It appeared that both TUNEL and annexin V methods are sensitive and specific and produced similar data in all measurements. The immunocytochemical detection of lamin B was less reliable than the other methods.
Kylarová
Dana
D
Department of Histology and Embryology, Faculty of Medicine, Palacky University, Olomouc, Czech Republic. nepozit@tunw.upol.cz
Procházková
Jirina
J
Mad'arová
Jana
J
Bartos
Jan
J
Lichnovský
Václav
V
D017304:Annexin A5 / Q000032:analysis; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D053938:DNA Fragmentation / Q000187:drug effects; D003609:Dactinomycin / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005047:Etoposide / Q000494:pharmacology; D005434:Flow Cytometry / Q000379:methods*; D019084:Fluorescent Antibody Technique, Indirect; D018922:HL-60 Cells; D006801:Humans; D020287:In Situ Nick-End Labeling / Q000379:methods*; D007701:Kinetin; D034921:Lamin Type B / Q000032:analysis; D011687:Purines / Q000494:pharmacology; D012680:Sensitivity and Specificity
D017304:Annexin A5; D004791:Enzyme Inhibitors; D034921:Lamin Type B; D011687:Purines; C118034:bohemine; D003609:Dactinomycin; C090046:olomoucine; D005047:Etoposide; D007701:Kinetin