Determination of hydroxyanthraquinoids in Rhubarb by cyclodextrin-modified micellar electrokinetic chromatography using a mixed micellar system of sodium dodecyl sulfate and sodium cholate.
A cyclodextrin-modified micellar electrokinetic chromatographic (CD-MEKC) method was established to determine five hydroxyanthraquinoids in Rhubarb. The five components were successfully separated by using the mixed micellar system consisting of 20 mmol/l sodium dodecyl sulfate (SDS) and 20 mmol/l sodium cholate (SC) with 10 mmol/l beta-cyclodextrin in phosphate buffer (pH 10.4). The separation was optimized by adjusting buffer pH, concentrations of beta-cyclodextrin and SC and applied voltage. The proposed method was validated and applied to the determination of two commercial Rhubarb samples. The results obtained were satisfactory.
Shang
Xiaoyu
X
Department of Chemistry, Institute of Applied Chemistry, Graduate School of the University of Science and Technology of China, Academia Sinica, Beijing 100039, People's Republic of China.
Yuan
Zhuobin
Z
D000880:Anthraquinones / Q000032:analysis*; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary; D003505:Cyclodextrins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012250:Rheum / Q000737:chemistry*; D020358:Sodium Cholate / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate; D013501:Surface-Active Agents
D000880:Anthraquinones; D002021:Buffers; D003505:Cyclodextrins; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D020358:Sodium Cholate