Positively charged sequences of human papillomavirus type 16 capsid proteins are sufficient to mediate gene transfer into target cells via the heparan sulfate receptor.
Using synthetic peptides we have shown that positively charged sequences present at the C terminus of the L1 protein and the N and C termini of the L2 protein of human papillomavirus type 16 (HPV-16) bind to both DNA and heparan sulfate receptors. Moreover, these short amino acid sequences are sufficient to mediate gene transfer in COS-7 cells. The L1 proteins of other HPVs were shown to contain one or two DNA- and heparin-binding sequences that have the capacity to transfer genes. These DNA-binding sequences also recognized the enhancing packaging sequence of bovine papillomavirus type 1. The results suggest that the L2 protein could participate in DNA packaging during maturation of virions.
Bousarghin
Latifa
L
Laboratoire de Virologie Moléculaire, INSERM EMIU 00-10 and USC INRA, Faculté des Sciences Pharmaceutiques 'Philippe Maupas', 31 avenue Monge, 37200 Tours, France.
Touzé
Antoine
A
Laboratoire de Virologie Moléculaire, INSERM EMIU 00-10 and USC INRA, Faculté des Sciences Pharmaceutiques 'Philippe Maupas', 31 avenue Monge, 37200 Tours, France.
Combita-Rojas
Alba-Lucia
AL
Laboratoire de Virologie Moléculaire, INSERM EMIU 00-10 and USC INRA, Faculté des Sciences Pharmaceutiques 'Philippe Maupas', 31 avenue Monge, 37200 Tours, France.
Coursaget
Pierre
P
Laboratoire de Virologie Moléculaire, INSERM EMIU 00-10 and USC INRA, Faculté des Sciences Pharmaceutiques 'Philippe Maupas', 31 avenue Monge, 37200 Tours, France.
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002213:Capsid / Q000737:chemistry* / Q000378:metabolism; D036022:Capsid Proteins; D004268:DNA-Binding Proteins / Q000378:metabolism; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006497:Heparan Sulfate / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D027383:Papillomaviridae / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014162:Transfection; D014771:Virion / Q000235:genetics / Q000378:metabolism
D036022:Capsid Proteins; D004268:DNA-Binding Proteins; D006497:Heparan Sulfate; D009856:Oncogene Proteins, Viral; D010455:Peptides; D011956:Receptors, Cell Surface; C063316:L2 protein, Human papillomavirus type 16; C063317:L1 protein, Human papillomavirus type 16