[Nucleotide sequence analysis of the minimal replicon of the Streptomyces plasmid pSGL1].
The high-copy-number plasmid pSGL1(7.4 kb) was isolated from Streptomyces globisporus. Deletion experiments showed its minimal replicon is located on the 2.0 kb Sau3 AI fragment. This fragment was subcloned. DNA sequence data analysis showed this fragment is a new sequence. Only an open reading frame with high coding probability is located on the minimal replicon. The deduced protein contains motifs characteristic of replicase for rolling-circle replication.
Zhang
H
H
Institute of Medicinal Biotechnology, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100050.
Hong
B
B
Li
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000737:chemistry*; D012093:Replicon; D013302:Streptomyces / Q000235:genetics*
C023140:DNA replicase; D004259:DNA-Directed DNA Polymerase