Design and characterization of enzymosomes with surface-exposed superoxide dismutase.
Superoxide dismutase (SOD) was chemically modified by covalent linkage of fatty acid chains to the accessible epsilon-amino groups of the enzyme. This acylation method gave rise to a different enzyme entity (Ac-SOD) as evidenced by different physicochemical properties such as octanol/water partition coefficient and isoelectric point (pI) as compared to SOD. Ac-SOD was incorporated in conventional and long-circulating liposomes (LCL) and characterized in terms of incorporation efficiency, protein to lipid ratio (Prot/Lip), enzymatic activity retention and zeta potential. The observation that Ac-SOD liposomes present enzymatic activity on their external surface indicates that these formulations can act independent of rate and extent of enzyme release as required in case of SOD liposomes. The decrease of superficial charge of liposomal formulations containing Ac-SOD, as compared to SOD liposomes, may be related to the negatively charged enzyme molecules localized on the liposome surface. The comparative characterization of Ac-SOD and SOD liposomal formulations evidenced that the two enzyme forms differ substantially regarding their intraliposomal location: SOD tends to be localized in the internal aqueous spaces, whereas Ac-SOD is expected to be localized in the lipid bilayers of the liposomes, partially buried into the outer surface and exposed to the external medium. These liposomal structures with surface-exposed SOD were designated as Ac-SOD enzymosomes. The properties of these enzymosomes may influence the therapeutic effect, as the release of the enzyme from extravasated vesicles is no longer a necessary requirement for achieving dismutating activity within the inflamed target site.
Gaspar
M M
MM
Departamento de Biotecnologia, Unidade Novas Formas de Agentes Bioactivos, Instituto Nacional de Engenharia e Tecnologia Industrial, Estrada do Paço do Lumiar 22, 1649-038 Lisbon, Portugal.
Martins
M B
MB
Corvo
M L
ML
Cruz
M E M
ME
D000215:Acylation; D000588:Amines; D004134:Dimyristoylphosphatidylcholine; D015195:Drug Design; D007526:Isoelectric Point; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D008970:Molecular Weight; D010316:Particle Size; D011092:Polyethylene Glycols; D013482:Superoxide Dismutase / Q000737:chemistry*; D013696:Temperature
D000588:Amines; D008051:Lipid Bilayers; D008081:Liposomes; D011092:Polyethylene Glycols; D013482:Superoxide Dismutase; C009317:stearylamine; D004134:Dimyristoylphosphatidylcholine