title: Radioprotective effect of novel disubstituted thioureas on pea (Pisum sativum L.) development.
abstract: The review presents our research on the influence of novel thiourea compounds on the biological and genetic effect of gamma-rays using in vivo and in vitro systems of pea. Some novel disubstituted thioureas: o-allylthioureidobenzoic acid (ATB); o-phenylthioureidobenzoic acid (PTB); N-allyl-N'-2-pyridylthiourea (A-2-PTU); N-phenyl-N'-2-pyridylthiourea (P-2-PTU) and 1,4-allylthioureidosalicylic acid (ATUS) were examined. Pea (Pisum sativum L.) seeds from five varieties were used. Experiments in vivo and in vitro were carried under laboratory, greenhouse and field conditions. The data revealed the PTB radioprotective effect demonstrated by: reduction of chromosome aberrations by 2 folds; 50% increase of germinating and surviving plants in M1; twice higher frequency of induced mutations in M2 generation relative to irradiation without PTB treatment; decreasing the level of induced radiation suppression leading to favorable effect on the initial stem and root development of pea. ATB radioprotective effect was demonstrated in vitro by: 25-35% stimulation of organogenesis; by 20-50% increase in bud formation; by 25% stimulation of growth. The effect of A-2-PTU and P-2-PTU depended on the irradiation dose. The protective effect of A-2-PTU is more pronounced at lower irradiation dose, while the effect of P-2-PTU is more pronounced at higher irradiation dose. ATUS, opposite to the other compounds, revealed radiosensibilizing effect by: 16-27% increase in lethality caused by gamma-rays leading to lower number of germinating and surviving plants in M1; 50% decrease in the number of induced mutations in M2 generation; limiting the types of induced mutations at the higher irradiation dose. As a result of the experiments useful mutation forms were obtained, characterized with: earliness, lodging and disease resistance; higher productivity.
authors.affiliation: Institute of Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria.
authors.forename: A
authors.initials: A
authors.lastname: Mehandjiev
authors.forename: G
authors.initials: G
authors.lastname: Kosturkova
authors.forename: G
authors.initials: G
authors.lastname: Vassilev
authors.forename: S
authors.initials: S
authors.lastname: Noveva
mesh_terms: D005720:Gamma Rays; D018532:Pisum sativum / Q000187:drug effects / Q000528:radiation effects*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013890:Thiourea / Q000494:pharmacology*
chemical_list: D011837:Radiation-Protective Agents; D013890:Thiourea