Stepwise surface regeneration of electrochemical immunosensors working on biocatalyzed precipitation.
A new strategy of stepwise surface regeneration for electrochemical immunosensors, working on a biocatalyzed precipitation reaction, has been developed. The strategy is based on the combination of deposited product thin-film dissolution and bound-protein displacement reactions from the modified sensor surfaces. As a model system, surfaces functionalized with biotin groups and their affinity recognition/ displacement reactions with antibiotin antibody molecules were chosen and investigated for affinity-sensing and stepwise regeneration reactions.
Yoon
Hyun C
HC
BioMEMS Group, Basic Research Laboratory, ETRI, Gajeong-dong, Yuseong-gu, Daejeon 305-350, Korea. hcyoon@etri.re.kr
Ko
Jong Soo
JS
Yang
Haesik
H
Kim
Youn Tae
YT
D000906:Antibodies / Q000032:analysis; D000915:Antibody Affinity; D015374:Biosensing Techniques; D001710:Biotin / Q000276:immunology
D000906:Antibodies; D001710:Biotin