[Genetic studies of 13 loci in Guangdong Han population by two multiplex PCR systems and its forensic application].
OBJECTIVE
The genetic studies of 13 short tandem repeats(STRs) loci in two multiplex amplification systems were carried out on Chinese Han population in Guangdong.


METHODS
DNA samples from 328 unrelated individuals were screened. The 13 loci were D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, TH01, TPOX and CSF1PO. The PCR products were analyzed and genotyped by ABI 377-96 Sequencer.


RESULTS
The combined power of discrimination (DP) was 0.999999999999993 and the combined paternity of exclusion(PE) was 99.999%. These 13 loci met the Hardy-Weinberg expectations.


CONCLUSIONS
The two multiplex amplification systems were very useful in forensic case investigation.
Li
Y
Y
Guangzhou Criminal Science & Technology Institute, Guangzhou 510030.
Wang
S B
SB
Liu
C
C
Li
H X
HX
Hu
H Y
HY
Liu
H
H
Liu
C H
CH
Chen
X H
XH
D044466:Asian People / Q000235:genetics; D002681:China; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D008297:Male; D010334:Paternity; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences