title: Hepatitis G viral RNA co-infection in plasma and peripheral blood mononuclear cells in patients with hepatitis C.
abstract: The incidence of the co-infection of hepatitis G virus (HGV) and hepatitis C virus (HCV) and its clinical implication was investigated and the difference in the positive rate of HGV RNA and HCV RNA between plasma and peripheral blood mononuclear cells (PBMCs) observed. By using reverse transcriptase polymerase chain reaction (RT-PCR) assay, HCV-RNA and HGV-RNA in plasma and PBMCs of 72 patients with hepatitis C was detected. It was showed that HGV RNA was positive in plasma of 11 patients, in PBMCs of 15 patients, and simultaneously in both of plasma and PBMCs of 10 patients with the co-infection rate being 22.2%. Nine patients were both HGV RNA and HCV RNA positive in plasma, 11 patients were both HGV RNA and HCV RNA positive in PBMC, and 6 patients were both HGV RNA and HCV RNA positive in both plasma and PBMC with the positive rate being 12.4%, 15.3% and 8.3% respectively. The positive rate of both HGV RNA and HCV RNA in PBMCs was higher than in plasma. It was concluded that the HGV co-infection rate in the patients with hepatitis C was 22.2%. Simultaneous examination of plasma and PBMC can improve clinically detectable rate.
authors.affiliation: Department of Infectious Diseases, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Zeng
authors.forename: D
authors.initials: D
authors.lastname: Luo
authors.forename: W
authors.initials: W
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Guo
authors.forename: X
authors.initials: X
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D018178:Flaviviridae Infections / Q000821:virology*; D029407:GB virus C / Q000302:isolation & purification*; D016174:Hepacivirus / Q000302:isolation & purification; D006526:Hepatitis C / Q000821:virology*; D006525:Hepatitis, Viral, Human / Q000821:virology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000097:blood*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015163:Superinfection / Q000821:virology
chemical_list: D012367:RNA, Viral