title: [Determination of beta-carotene in tobacco by HPTLC].
abstract: This paper reports the high performance thin-layer chromatography (HPTLC) determination of beta-carotene in tobacco. The developer used was V(benzene):V(acetone) = 4:1. The detection wavelength was 450 nm. The linear range and the detectable limit of the method were 50 ng-0.5 microgram and 20 ng per spot of beta-carotene respectively. The method has been proved to be simple, sensitive, accurate and reproducible.
authors.affiliation: Hefei Institute of Economics and Technology, Hefei 230052, China.
authors.forename: L
authors.initials: L
authors.lastname: Shi
authors.forename: X L
authors.initials: XL
authors.lastname: Zhu
authors.forename: B Z
authors.initials: BZ
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
mesh_terms: D002855:Chromatography, Thin Layer / Q000379:methods*; D014026:Nicotiana / Q000737:chemistry*; D019207:beta Carotene / Q000032:analysis*
chemical_list: D019207:beta Carotene