Preparation and analysis of spermatocyte meiotic pachytene bivalents of pigs for gene mapping.
Well-spread meiotic pachytene bivalents were obtained by using the prolonged hypotonic treatment combined with high chloroform Carnory's fixative solution from cells of the testes of domestic pigs. Comparison in the division index and length of pachytene bivalents with metaphase chromosomes showed that those of the former are 5 times higher and 3.42(1.87-5.98) times longer than those of the latter. Comparative studies on chromomere maps of bivalents and mitotic chromosomal G-bands were conducted by using the chromosome 12 as a example. Sex vesicle and various shapes of synaptic sex chromosomes have been observed. Two-color PRimed IN Situ (PRINS) labeling has been conducted successfully on pachytene bivalents of pigs.
Liu
Bang
B
Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Jin
Geng Lin
GL
Zhao
Shu Hong
SH
Yu
Mei
M
Xiong
Tong An
TA
Peng
Zhong Zhen
ZZ
Li
Kui
K
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D002874:Chromosome Mapping / Q000379:methods*; D023902:Chromosome Pairing / Q000235:genetics; D002875:Chromosomes / Q000235:genetics*; D007621:Karyotyping; D008297:Male; D008540:Meiosis / Q000235:genetics*; D008677:Metaphase / Q000235:genetics; D012730:Sex Chromosomes / Q000235:genetics; D013090:Spermatocytes / Q000166:cytology* / Q000378:metabolism; D034421:Sus scrofa / Q000235:genetics*; D013737:Testis / Q000166:cytology / Q000378:metabolism