title: Proliferative ability and alkaline phosphatase activity with in vivo cellular aging in human pulp cells.
abstract: Little is known about the effect of aging on characteristic functions of pulp cells. When damaged pulp is recovered and mineralized tissue is formed to protect remaining pulp tissue, the general responses of pulp tissue after adequate stimuli (pulp cell proliferation and activation of alkaline phosphatase [ALPase]) are thought to be essential. In this study, we compared proliferative ability and ALPase activity between cultures of human pulp (HP) cells obtained from young and aged donors. The in vitro proliferative lifespan of HP cells from young donors was longer than HP cells from aged donors. Growth rates and ALPase activity of HP cells decreased with increasing donor age. These findings suggest that impaired repair of pulp and dentin in aged patients is partly due to a decrease in the proliferative ability and ALPase activity in aged pulp cells.
authors.affiliation: Department of Periodontology and Endodontology, Hiroshima University, Faculty of Dentistry, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Shiba
authors.forename: Keiji
authors.initials: K
authors.lastname: Nakanishi
authors.forename: Fahmida
authors.initials: F
authors.lastname: Rashid
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Mizuno
authors.forename: Takamune
authors.initials: T
authors.lastname: Hino
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Ogawa
authors.forename: Hidemi
authors.initials: H
authors.lastname: Kurihara
mesh_terms: D000293:Adolescent; D000368:Aged; D000469:Alkaline Phosphatase / Q000378:metabolism; D002455:Cell Division; D016922:Cellular Senescence / Q000502:physiology*; D002648:Child; D003782:Dental Pulp / Q000166:cytology* / Q000201:enzymology; D006801:Humans; D008875:Middle Aged
chemical_list: D000469:Alkaline Phosphatase