title: Tumor sensitivity to anti-cancer drugs predicted by changes in fluorescence intensity and polarization in vitro.
abstract: BACKGROUND
  We used the CellScan, a novel static cytometer, to monitor changes induced by anti-neoplastic drugs in the fluorescence intensity and polarization of fluorescently-labeled tumor cells.
  
  
  METHODS
  T47D and T80 human breast cancer cell lines were exposed to navelbine and to 5-fluorouracil and the fluorescence properties of the treated cells, stained with fluorescein diacetate and rhodamine 123, were measured by the CellScan.
  
  
  RESULTS
  A strong correlation was found between the inhibition of cell growth induced by the two drugs, as estimated from cell counts, and the resulting changes in fluorescence intensity and polarization, as monitored by the CellScan. Fluorescence hyperpolarization of the labeled cells occurred in conjunction with AnnexinV binding and propidium iodide exclusion, indicating that such hyperpolarization, resulting from drug action, reflects an early stage of apoptosis, as previously proposed.
  
  
  CONCLUSIONS
  The system presented here could serve as the basis for assessing drug sensitivity or resistance of cancer cells derived from small biopsies of solid human tumors, thus eliminating prior tumor culturing and time-consuming assays.
authors.affiliation: Medis-El Ltd., Shabazi 14, Yehud 56101, Israel. yael@medisel.co.il
authors.forename: Yael S
authors.initials: YS
authors.lastname: Schiffenbauer
authors.forename: Ella
authors.initials: E
authors.lastname: Trubniykov
authors.forename: Ben-Tzion
authors.initials: BT
authors.lastname: Zacharia
authors.forename: Smadar
authors.initials: S
authors.lastname: Gerbat
authors.forename: Zvi
authors.initials: Z
authors.lastname: Rehavi
authors.forename: Gideon
authors.initials: G
authors.lastname: Berke
authors.forename: Samario
authors.initials: S
authors.lastname: Chaitchik
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005452:Fluoresceins / Q000378:metabolism; D005454:Fluorescence Polarization / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D011237:Predictive Value of Tests; D011419:Propidium / Q000378:metabolism; D020112:Rhodamine 123 / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000077235:Vinorelbine
chemical_list: D017304:Annexin A5; D000970:Antineoplastic Agents; D005452:Fluoresceins; D005456:Fluorescent Dyes; D020112:Rhodamine 123; D011419:Propidium; D014747:Vinblastine; D000077235:Vinorelbine; D005472:Fluorouracil; C018506:diacetylfluorescein