title: A computer image analysis system for microvessel density measurement in solid tumours.
abstract: Microvessel density (MVD) is a widely used surrogate measure of angiogenesis in pathological specimens and tumour models. Measurement of MVD can be achieved by several methods. Automation of counting methods aims to increase the speed, reliability and reproducibility of these techniques. The image analysis system described here enables MVD measurement to be carried out with minimal expense in any reasonably equipped pathology department or laboratory. It is demonstrated that the system translates easily between tumour types which are suitably stained with minimal calibration. The aim of this paper is to offer this technique to a wider field of researchers in angiogenesis.
authors.affiliation: Department of Urology, University Hospitals of Leicester NHS Trust, Leicester, UK. jonathan@jcgoddard.freeserve.co.uk
authors.forename: Jonathan Charles
authors.initials: JC
authors.lastname: Goddard
authors.forename: Christopher Derek
authors.initials: CD
authors.lastname: Sutton
authors.forename: Peter N
authors.initials: PN
authors.lastname: Furness
authors.forename: Roger Clive
authors.initials: RC
authors.lastname: Kockelbergh
authors.forename: Kenneth John
authors.initials: KJ
authors.lastname: O'Byrne
mesh_terms: D001808:Blood Vessels / Q000473:pathology*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D009369:Neoplasms / Q000098:blood supply*; D001749:Urinary Bladder Neoplasms / Q000473:pathology*