title: Expression of mRNA transcripts of the Na+/H+ and Cl-/HCO3- exchanger isoforms in human nasal mucosa.
abstract: Electrolyte transport by nasal epithelia has been suggested to be important for controlling the quantity and composition of the nasal fluid and may play an important role in the development of nasal polyps. One of a number of mechanisms involving translocation of Na+ and Cl- across cell membranes includes electroneutral processes, such as Na+/H+ exchange (NHE) and Cl-/HCO3- exchange (AE). The present study evaluated the presence of mRNAs for various members of the human NHE and AE gene families in human inferior turbinate mucosa and nasal polyp using reverse transcriptase polymerase chain reaction and in situ hybridization. The mRNA for NHE1 was detected in human turbinate mucosa and nasal polyp while the mRNAs for NHE2 and NHE3 could not be detected in any of the samples examined. Of the AE isoforms, AE2 mRNA was expressed in inferior turbinate mucosa but not in nasal polyp. In situ hybridization revealed that NHE1 mRNA in the turbinate mucosa and nasal polyp was localized in the epithelial layer and submucosal glands. AE2 mRNA was also expressed in the epithelial layer and submucosal glands of inferior turbinate mucosa. Taken together, these results indicate that the expression of AE2 mRNA is altered in nasal polyp compared with inferior turbinate mucosa, suggesting that the altered expression of these genes in nasal polyp may cause impaired electrolyte and water transport across the epithelial cells.
authors.affiliation: Department of Otorhinolaryngology-Head & Neck Surgery, College of Medicine, Korea University, Seoul, South Korea.
authors.forename: Sang Hag
authors.initials: SH
authors.lastname: Lee
authors.forename: Youn Sik
authors.initials: YS
authors.lastname: Seok
authors.forename: Hak Hyun
authors.initials: HH
authors.lastname: Jung
authors.forename: Byung Hoon
authors.initials: BH
authors.lastname: Oh
authors.forename: Heung Man
authors.initials: HM
authors.lastname: Lee
authors.forename: Soon Young
authors.initials: SY
authors.lastname: Kwon
authors.forename: Kwang Yoon
authors.initials: KY
authors.lastname: Jung
mesh_terms: D000328:Adult; D027963:Chloride-Bicarbonate Antiporters / Q000032:analysis* / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000737:chemistry*; D009298:Nasal Polyps / Q000378:metabolism; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017923:Sodium-Hydrogen Exchangers / Q000032:analysis* / Q000235:genetics
chemical_list: D027963:Chloride-Bicarbonate Antiporters; D020033:Protein Isoforms; D012333:RNA, Messenger; D017923:Sodium-Hydrogen Exchangers