Development of an aspartic acid-based cross-linking monomer for improved bioseparations.
Improved specificity and binding affinity by molecularly imprinted polymers is possible by development of novel functional materials. Furthermore, increasing the cross-link density of imprinted polymers by using cross-linking functional groups was anticipated to improve polymer molecular recognition. A novel cross-linking monomer derived from an L-aspartic acid precursor was synthesized and employed in molecularly imprinted polymers to mimic more closely the scaffolding of proteins, and thus provide more protein-like selectivity. Chromatographic results revealed a more than 7-fold improvement in polymers imprinted using the new monomer versus a traditionally formulated polymer imprinted with methacrylic acid as the functional monomer.
Spivak
D A
DA
Department of Chemistry, Louisiana State University, 232 Choppin Hall, Baton Rouge, LA 70803, USA.
Sibrian-Vazquez
M
M
D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004950:Esterases / Q000737:chemistry / Q000302:isolation & purification*; D008099:Liver / Q000201:enzymology; D008689:Methacrylates / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D013552:Swine
D003432:Cross-Linking Reagents; D008689:Methacrylates; C008384:methacrylic acid; D001224:Aspartic Acid; D004950:Esterases