[Cloning and expression of the gene encoding maltoologosyl trehalose synthase from Sulfolobus shibatae in E. coli].
2.2 kb DNA fragment encoding a novel enzyme, maltooligosyl trehalose synthase (MTSase) was amplified from Sulfolobus shibatae by using PCR technique. The amplified 2.2 kb DNA fragment was inserted into an expression vector, pBV220, to yield the recombinant plasmid pSBGT1. MTSase gene in pBSGT1 was expressed in E. coli. The molecular weight of expressed MTSase detected by SDS-PAGE was about 74 kD, which is conformed with that deduced from nucleotide sequence. The expressed MTSase protein accounted for about 4.4% of the total cell protein. The MTSase from transformants containing pBSGT1 is capable of decreasing DE value, forming non-reducing or less-reducing saccharides when allowed to act on reducing partial starch hydrolysates.
Chen
W
W
Laboratory of Enzymology Institute of Microbiology, Chinese Academy of Science, Beijing 100080.
Liu
L
L
Sun
P
P
Jin
C
C
D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005964:Glucosyltransferases / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D016944:Sulfolobus / Q000201:enzymology*
D005964:Glucosyltransferases; C099084:maltooligosyl trehalose synthase