[IL-2 gene and cisplatin combined therapy for head and neck squamous cell carcinoma].
OBJECTIVE
The objective of this study is to assess the efficacy of IL-2 gene and cisplatin combined therapy on murine HNSCC models.


METHODS
HNSCC tumors were established in murine models. The sizes of tumors were measured before and after combined IL-2 and cisplatin treatment, and the results were evaluated by comparing different single treatment groups and the control IL-2 expression was measured after IL-2 gene transfer. Cytotoxic T-lymphocyte (CTL) and natural killer (NK) assays were also performed.


RESULTS
HNSCC tumor growth was significantly inhibited following combined IL-2 gene and cisplatin therapy, when compared with that of the control. The levels of IL-2 protein expressions were increased in combined and single IL-2 treated groups. The activities of cytotoxic T-lymphocytes and natural killers in both the combined treatment group and the IL-2 treated group are higher than those of the controlled group.


CONCLUSIONS
Combined IL-2 gene and cisplatin therapy can significantly inhibit HNSCC tumor growth in the murine models, and efficiently induces anti-tumor immune reaction of hosts.
Liu
S
S
Department of Otolaryngology, First Affiliated Hospital, West China University of Medical Sciences.
Lin
Y
Y
Tang
S
S
Yang
H
H
Liang
C
C
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000628:therapy*; D002945:Cisplatin / Q000008:administration & dosage*; D003131:Combined Modality Therapy; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D006258:Head and Neck Neoplasms / Q000276:immunology / Q000628:therapy*; D007376:Interleukin-2 / Q000235:genetics*; D007694:Killer Cells, Natural / Q000187:drug effects; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects
D000970:Antineoplastic Agents; D007376:Interleukin-2; D002945:Cisplatin