title: An LOH and mutational investigation of the ST7 gene locus in human esophageal carcinoma.
abstract: Frequent loss of heterozygosity (LOH) on human chromosome 7q31 has been reported in numerous malignancies. Suppressor of tumorigenicity 7 (ST7) has been identified as a candidate tumor suppressor gene in this region. To identify whether 7q31 and genetic alterations of ST7 were involved in human esophageal carcinogenesis, we performed LOH mapping of a 5.4 cM region at 7q31-q35 in 43 primary esophageal carcinomas, as well as mutational analyses of the ST7 gene in tumors with LOH in this region. Of 43 tumors, 12 (28%) showed LOH at 7q31-q35. These included four (22%) of 18 squamous cell carcinomas and eight (32%) of 25 adenocarcinomas. The peak LOH locus was D7S480, lying 4.2 Mb telomeric to ST7 and showing LOH in eight of 37 informative tumors, or 22%. No mutations were found in the entire coding or flanking intronic regions of the ST7 gene among 12 tumors with 7q-LOH. In addition, quantitative RT-PCR analyses of ST7 mRNA expression levels in 11/13 normal-tumor pairs failed to show more than a 50% decrease in tumor ST7 mRNA relative to matched normal tissues. These data suggest that LOH at 7q31-q35 is involved in the origin or progression of at least a subset of esophageal carcinomas, but that ST7 is not the target gene of this somatic event.
authors.affiliation: Department of Medicine, Division of Gastroenterology and Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore 21201, USA.
authors.forename: Suna
authors.initials: S
authors.lastname: Wang
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Mori
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Sato
authors.forename: Jing
authors.initials: J
authors.lastname: Yin
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Tong-Tong
authors.initials: TT
authors.lastname: Zou
authors.forename: Andreea
authors.initials: A
authors.lastname: Olaru
authors.forename: Martha C
authors.initials: MC
authors.lastname: Kimos
authors.forename: Kellie
authors.initials: K
authors.lastname: Perry
authors.forename: Florin M
authors.initials: FM
authors.lastname: Selaru
authors.forename: Elena
authors.initials: E
authors.lastname: Deacu
authors.forename: Menghong
authors.initials: M
authors.lastname: Sun
authors.forename: Ying-Chang
authors.initials: YC
authors.lastname: Shi
authors.forename: David
authors.initials: D
authors.lastname: Shibata
authors.forename: John M
authors.initials: JM
authors.lastname: Abraham
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Greenwald
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Meltzer
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics; D002277:Carcinoma / Q000235:genetics*; D002897:Chromosomes, Human, Pair 7; D021901:DNA, Intergenic; D004938:Esophageal Neoplasms / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007438:Introns; D019656:Loss of Heterozygosity; D009154:Mutation; D018307:Neoplasms, Squamous Cell / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D040641:Quantitative Trait Loci; D012016:Reference Values; D017422:Sequence Analysis, DNA; D025521:Tumor Suppressor Proteins
chemical_list: D021901:DNA, Intergenic; D011506:Proteins; C412123:ST7 protein, human; D025521:Tumor Suppressor Proteins