[A method of preparing the section of eye tissue for transmission electron microscopy].
A method of preparing the section of eye tissue for transmission electron microscopy (TEM) is recommended. The tissue was prefixed with a mixed solution of 4% paraformaldehyde and 2.5% glutaraldehyde and followed by softening in 3% EDTA solution for 20 minutes, then the tissue was fixed in 1% osmium tetroxide, dehydrated in series acetone, infiltrated in Epox 812 for a longer, and embeded. Ultrathin sections were cut with glass knives or diamond knife, stained with uranyl acetate and lead citrate and examined with H600-IV. The advantage of this method is that the ultrastructures of tissues are well preserved without any damage and distortion. The main points of the procedure of preparation have been discussed.
Tang
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Electron Microscopy Laboratory, West China University of Medical Sciences, Chengdu 610041.
Tang
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Gan
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D
D000818:Animals; D005123:Eye / Q000648:ultrastructure*; D066298:In Vitro Techniques; D017348:Microscopy, Electron, Scanning Transmission; D008867:Microtomy / Q000379:methods*; D051381:Rats; D013194:Staining and Labeling / Q000379:methods; D016610:Tissue Embedding / Q000379:methods