[Study on HCMV replication in gene transtecfed cells].
Five gene transfected cells (GTC: A5, B3, D3, G8 and H3) were generated by DNA (from HEP-2) transfer (into HEL). Chromosome number of the transfectants fell between that of HEL and the combined chromosome number of the two cell lines. D3 showed more positive fluorescence cells than A5, B3, G8 and H3 by 4d after infection of HCMV. As HCMV infection dose was increased from 0.1 to 100 PFU/ml, significant increase of viral production was observed. The similar susceptibility to HCMV was shown in different passages of D3. The immortal D3 may provide a tool to analyze host cell factors controlling the transcription and replication of HCMV.
Yan
H
H
School of Medicine, University of Yangzhou, Yangzhou 225001.
Shen
H
H
Zhang
Y
Y
Geng
J
J
D002460:Cell Line; D002875:Chromosomes; D003587:Cytomegalovirus / Q000502:physiology*; D006801:Humans; D014162:Transfection; D014779:Virus Replication