Rapid analysis of terpene lactones in extract of Ginkgo biloba L. by high performance liquid chromatography.
A new rapid analytical method was developed for the quantification of terpene lactones (bilobalide(BB) and ginkgolide A, B, C, J) in extract of Ginkgo biloba L. (EGb) using a liquid-liquid solvent extraction procedure followed by high performance liquid chromatography. EGb was dissolved in 30% ethanol and extracted with ether. After evaporation, the residue was then determined by HPLC on a C18 column with methanol-water-orthophosphoric acid (25:75:0.1, V/V) as eluent and refractive index (RI) detection. Results showed that the excellent sample clean-up procedure is more simple and specific, and saves more time (less than 20 min) than any other methods that have been reported, and also leads to high recoveries (> 99.0%) and low RSDs (< 2.0%). The reproducible method is regarded to be very useful for evaluating the quality of extract of Ginkgo biloba L.
Wang
H F
HF
Anal. & Test Centre of Cereal, Oil & Foodstuffs, Nanjing Institute of Economics, Nanjing 210003, China.
Ju
X R
XR
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003517:Cyclopentanes / Q000032:analysis*; D004224:Diterpenes; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005663:Furans / Q000032:analysis*; D020441:Ginkgo biloba / Q000737:chemistry*; D046934:Ginkgolides; D007783:Lactones / Q000032:analysis*; D013729:Terpenes / Q000032:analysis
D003517:Cyclopentanes; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D005663:Furans; D046934:Ginkgolides; D007783:Lactones; D013729:Terpenes; C058295:ginkgolide C; C045856:ginkgolide B; C075228:ginkgolide J; C073710:bilobalide