title: Regulation, secretion and activity of type III-secreted proteins of enterohaemorrhagic Escherichia coli O157.
abstract: Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 causes gastrointestinal disease with the potential for life-threatening sequelae. Although Shiga-like toxins are responsible for much of the serious pathology in humans, the bacterium also possesses a type III protein secretion system that is responsible for intimate attachment to host intestinal mucosa. This sophisticated interaction requires co-ordination that is governed by environmental and genetic factors. Ongoing research supports the following model for how EHEC enables and controls this process: (i) specific environmental cues that are present in the host result in the expression of a number of adhesins, including fimbriae, which allow the initial binding to the mucosal surface. The same conditions support the expression of the basal type III secretion apparatus; (ii) targeting and assembly of the translocon requires both an mRNA signal and chaperones, with coupled translation and secretion of translocon proteins, EspA, B and D; (iii) opening up of a conduit between the bacterium and host cell releases a cytoplasmic pool of effector proteins. A consequence of this is increased expression of particular effector proteins. Potentially, different proteins could be released into the cell at different times or have activities modulated with time; (iv) intimate contact between the translocated intimin receptor (Tir) and the bacterial surface factor intimin requires translocon expression to be down-regulated and translocon filaments to be lost. Fluorescent protein fusions allow contact-mediated regulation and protein targeting through the type III secretion system to be studied in detail.
authors.affiliation: Zoonotic and Animal Pathogens Research Laboratory, Medical Microbiology, Teviot Place, University of Edinburgh, Edinburgh EH8 9AG, UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: Roe
authors.forename: D E E
authors.initials: DE
authors.lastname: Hoey
authors.forename: D L
authors.initials: DL
authors.lastname: Gally
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism / Q000502:physiology*; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D015536:Down-Regulation; D004927:Escherichia coli Infections; D019453:Escherichia coli O157 / Q000378:metabolism* / Q000472:pathogenicity*; D007249:Inflammation; D008954:Models, Biological; D014774:Virulence
chemical_list: D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins