title: Solution structure of paralytic peptide of silkworm, Bombyx mori.
abstract: Paralytic peptide of Bombyx mori (BmPP) is one of the multifunctional ENF-peptides; the name of "ENF" is the consensus N-terminal amino acid sequence of the family peptides. We revealed that BmPP significantly possesses growth-blocking activity and plasmatocyte-spreading activity and that its activity profiles are different from those of another ENF-family peptide, namely, the growth-blocking peptide of Pseudaletia separata (PsGBP). We also determined the NMR structures of BmPP and PsGBP under the same conditions, which revealed the structural differences of the first and second beta-turn regions between the two peptides. On the basis of our results, it can be considered that the tertiary structural difference in these peptides may cause their different profiles of growth-blocking activity.
authors.affiliation: Bio-oriented Technology Research Advancement Institution, 1-40-2 Nisshin, Saitama, Saitama 331-8537, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Miura
authors.forename: Manabu
authors.initials: M
authors.lastname: Kamimura
authors.forename: Tomoyasu
authors.initials: T
authors.lastname: Aizawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Kiuchi
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Hayakawa
authors.forename: Mineyuki
authors.initials: M
authors.lastname: Mizuguchi
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kawano
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D012831:Bombyx / Q000737:chemistry* / Q000502:physiology; D016207:Cytokines / Q000032:analysis / Q000737:chemistry; D019476:Insect Proteins / Q000032:analysis / Q000737:chemistry; D007814:Larva / Q000737:chemistry / Q000502:physiology; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000032:analysis / Q000737:chemistry* / Q000502:physiology
chemical_list: D016207:Cytokines; D019476:Insect Proteins; D009479:Neuropeptides; C068690:growth blocking peptide, Apanteles kariyai; C121924:paralytic peptide, insect