[Effect of diet composition on expression of uncoupling protein-1 gene of rat].
In order to explore the effect of different diet composition on uncoupling proteins 1(UCP1) gene expression in brown adipose tissue, male Wistar rats are fed with different diets (basic diet, high protein diet, high fat diet, high calorie diet 1 and 2) for 2 months. The effects of different diets on weight and the ratio of fat mass to body weight are observed. Reverse transcription-polymerase chain reaction and western blotting technique are used to measure UCP1 mRNA and protein content of rat brown adipose tissue(BAT). The results show that body weight and the ratio of fat mass to body weight in high calorie 1 and 2 diet groups are obviously higher than those in groups of basic diet, high protein, and high fat diet (P < 0.05), but there is no significant difference among the last three groups (P > 0.05). UCP1 gene expression increases in both high calorie diet 1 group and 2 group, but it is not significantly different among basic diet, high protein and high fat diet groups with equal energy, indicating that energy can induce UCP1 gene expression of rat BAT.
Fu
Rongxia
R
College of Public Health of Harbin Medical University, Harbin 150001, China.
Sun
Changhao
C
Sun
Wenguang
W
Tang
Yalan
Y
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D007473:Ion Channels; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D024101:Mitochondrial Proteins; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D004041:Dietary Fats; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; C000604373:Ucp1 protein, rat; D000071256:Uncoupling Protein 1