Modulation of J774.1 macrophage L-arginine metabolism by intracellular Mycobacterium bovis BCG.
Using a Mycobacterium bovis BCG mutant (AS1) lacking a Bacillus subtilis L-arginine transporter homolog, we demonstrate here that the interaction between intracellular mycobacteria and the macrophage with respect to L-arginine transport and metabolism is quite complex. Intracellular AS1 stimulates macrophage L-arginine transport and accumulates 2.5-fold more (3)H label derived from L-arginine than does the wild type. These studies suggest that the accumulation of (3)H label reflects the acquisition of metabolites of L-arginine produced by the macrophage.
Peteroy-Kelly
Marcy A
MA
Department of Microbiology and Molecular Genetics, International Center for Public Health, UMDNJ/New Jersey Medical School, Newark 07101-1709, USA.
Venketaraman
Vishwanath
V
Talaue
Meliza
M
Seth
Anjali
A
Connell
Nancy D
ND
D026905:Amino Acid Transport Systems / Q000235:genetics; D026923:Amino Acid Transport Systems, Basic; D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001692:Biological Transport; D002460:Cell Line; D003470:Culture Media; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology*; D051379:Mice; D009154:Mutation; D009163:Mycobacterium bovis / Q000235:genetics / Q000378:metabolism*; D014316:Tritium / Q000378:metabolism
D026905:Amino Acid Transport Systems; D026923:Amino Acid Transport Systems, Basic; D003470:Culture Media; D014316:Tritium; C043909:arginine permease; D001120:Arginine