title: Enhanced expression of Duffy antigen in the lungs during suppurative pneumonia.
abstract: Duffy antigen is a chemokine binding protein expressed on the surface of erythrocytes and postcapillary venular endothelial cells. It binds selective CXC and CC chemokines with high affinity. Although Duffy antigen is present in the normal pulmonary vascular bed, it is not known whether its expression is altered by innate inflammatory responses in the lungs. We studied Duffy antigen expression by immunohistochemistry in autopsy lung specimens from 16 cases of suppurative pneumonia, 11 cases of acute lung injury, and seven normal lungs. In lungs with suppurative pneumonia, Duffy antigen was expressed in higher numbers of pre- and postcapillary parenchymal vessels compared to normal specimens or specimens with acute lung injury (p<0.03 and p<0.02, respectively). Lungs with suppurative pneumonia also showed Duffy antigen expression on the alveolar septa, whereas this was a rare finding in normal specimens or in acute lung injury (p<0.02). Furthermore, Duffy antigen labeling of the alveolar septa localized to regions with airspace accumulation of neutrophil-rich exudates. In summary, Duffy antigen expression is increased in the vascular beds and alveolar septa of the lung parenchyma during suppurative pneumonia, suggesting that Duffy antigen may have a functional role in the lung parenchyma during inflammation.
authors.affiliation: Department of Medicine, Division of Pulmonary & Critical Care Medicine, University of Washington School of Medicine, Seattle, USA.
authors.forename: Janet S
authors.initials: JS
authors.lastname: Lee
authors.forename: Charles W
authors.initials: CW
authors.lastname: Frevert
authors.forename: David R
authors.initials: DR
authors.lastname: Thorning
authors.forename: Stephan
authors.initials: S
authors.lastname: Segerer
authors.forename: Charles E
authors.initials: CE
authors.lastname: Alpers
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Cartron
authors.forename: Yves
authors.initials: Y
authors.lastname: Colin
authors.forename: Venus A
authors.initials: VA
authors.lastname: Wong
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Martin
authors.forename: Richard B
authors.initials: RB
authors.lastname: Goodman
mesh_terms: D001980:Bronchi / Q000098:blood supply / Q000378:metabolism; D004375:Duffy Blood-Group System / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000098:blood supply* / Q000378:metabolism*; D008833:Microcirculation; D011014:Pneumonia / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000098:blood supply / Q000378:metabolism; D013492:Suppuration
chemical_list: D004375:Duffy Blood-Group System