Cardiac mitochondrial complex activity is enhanced by heat shock proteins.
1. Prolonged ischaemia and reperfusion in heart transplantation results in mitochondrial dysfunction and loss of cardio-energetics. Improved myocardial tolerance to ischaemia-reperfusion can be increased by de novo synthesis of heat shock protein (Hsp) groups, transiently expressed following mild hyperthermic or oxidative stress. Consideration of the roles of various Hsp in ischaemic-reperfused myocardium can provide new insights into potential therapeutic adjuncts to cardiac surgery. 2. Several Hsp classes have been located within or in association with mitochondrial elements. Cardiac Hsp research has focused primarily on the 70 kDa group, involved in protein folding functions within the cytosol and matrix. Similarly, Hsp 60 and 10 have been shown to form a mitochondrial chaperonin complex conferring protection to ischaemia-challenged myocytes. Equally pertinent is Hsp 32, an isoform of the haem-metabolizing enzyme heme oxygenase. 3. Our studies have shown that mitochondrial respiratory enzyme activity can be protected by Hsp, affording protection to cardiac energetics during preservation for transplantation. Upregulation of Hsp 32, 60 and 72 in rats, achieved by mild hyperthermic stress, improved cardiac function, ultrastructure and mitochondrial respiratory and complex activities in ex vivo perfused hearts subjected to cold cardioplegic arrest and ischaemia-reperfusion. Pre-ischaemic mitochondrial complex activities were increased in heat stress versus sham-treated groups for complex I, IV and V. 4. Investigation of the direct effect of upregulation of Hsp 72 by gene transfection resulted in a similar pattern of response, with increased complex I activity and improved ventricular function. 5. These studies provide the first evidence of Hsp-mediated enhancement of mitochondrial energetic capacity. Enhanced protection of mitochondrial energetics, as a result of increased Hsp expression, contributes to the recovery of myocardial function in ischaemia-reperfusion.
Sammut
Ivan A
IA
Department of Pharmacology and Toxicology, University of Otago Faculty of Medicine, Dunedin, New Zealand. ivan.sammut@stonebow.otago.ac.nz
Harrison
Joanne C
JC
D000818:Animals; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D018869:Heat-Shock Response / Q000502:physiology; D006801:Humans; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism; D009206:Myocardium / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism*
D006360:Heat-Shock Proteins