title: Broad-range ribosomal RNA real-time PCR after removal of DNA from reagents: melting profiles for clinically important bacteria.
authors.affiliation: School of Medical Technology and Graduate Institute of Medical Biotechnology, Chang Gung University, Tao-Yuan 333, Taiwan, Republic of China.
authors.forename: Ching-Ping
authors.initials: CP
authors.lastname: Tseng
authors.forename: Ju-Chien
authors.initials: JC
authors.lastname: Cheng
authors.forename: Ching-Chung
authors.initials: CC
authors.lastname: Tseng
authors.forename: Cheyien
authors.initials: C
authors.lastname: Wang
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Chen
authors.forename: Daniel Tsun-Yee
authors.initials: DT
authors.lastname: Chiu
authors.forename: How-Chin
authors.initials: HC
authors.lastname: Liao
authors.forename: Shy-Shin
authors.initials: SS
authors.lastname: Chang
mesh_terms: D052160:Benzothiazoles; D004269:DNA, Bacterial / Q000302:isolation & purification*; D003850:Deoxyribonuclease I; D003959:Diamines; D004926:Escherichia coli / Q000235:genetics; D005456:Fluorescent Dyes / Q000302:isolation & purification*; D007202:Indicators and Reagents / Q000302:isolation & purification*; D009930:Organic Chemicals; D016133:Polymerase Chain Reaction; D011804:Quinolines; D012329:RNA, Bacterial / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000032:analysis*
chemical_list: D052160:Benzothiazoles; D004269:DNA, Bacterial; D003959:Diamines; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009930:Organic Chemicals; D011804:Quinolines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C098022:SYBR Green I; D003850:Deoxyribonuclease I