[Expression, purification and characterization of fruA, a transcription factor in Myxococcus xanthus].
FruA is a transcription factor essential for the development of Myxocoxccus xanthus. Gene encoding fruA with a poly-histidine tag was expressed in E. coli and simply purified by chromatography on nickel column. Data from gel retardation assay suggest that FruA regulates transcription of target genes in collaboration with other factors.
Mao
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X
Department of Biochemistry, Nanjing Railway Medical College, Nanjing 210009.
Ding
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L
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D002845:Chromatography; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D016941:Myxococcus xanthus / Q000235:genetics*; D010957:Plasmids; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*
D001426:Bacterial Proteins; C104848:FruA protein, Myxococcus xanthus; D014157:Transcription Factors