[Tn5 mutagenesis and the characteristics of indole-3-acetic acid biosynthesis in Alcaligenes faecalis A1501].
The IAA production of Alcaligenes faecalis wild type strain A1501 depended on addition of L-tryptopham(trp). A1501 could grow well in minimal medium without Trp, but could not produce IAA. The results indicated that there was Trp-dependent IAA biosynthesis pathway in A. faecalis A1501. A1501 shows a characteristic of cell density-dependent IAA biosynthesis. The Tn5-induced mutant bank of A1501 was constructed using Tn5 mutagenesis and one Trp auxotrophic mutant AT63 was selected and identified. The Tn5-induced mutant AT63 could synthesis 2.24 micrograms/mL IAA in minimal medium without L-Trp. It indicated there were at least two IAA biosynthesis pathways in A. faecalis, one needed Trp, the other needed IGP as precursor. The results of southern hybridization also suggested that the Tn5-insertion might locate in gene locus encoded IAA synthetase.
Li
F
F
Institute for Application of Atomic Energy, CAAS, Beijing 100094.
Ping
S
S
Su
B
B
Lin
M
M
D042481:Alcaligenes faecalis / Q000235:genetics / Q000378:metabolism*; D015139:Blotting, Southern; D004251:DNA Transposable Elements / Q000235:genetics*; D007210:Indoleacetic Acids / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D009154:Mutation; D014367:Tryptophan Synthase / Q000235:genetics
D004251:DNA Transposable Elements; D007210:Indoleacetic Acids; C030737:indoleacetic acid; D014367:Tryptophan Synthase