[Effects of human chorionic gonadotropin on cellular cAMP levels in human gastro-adenocarcinoma SGC-7901 cells].
The purpose of this study was to determine the effects of hCG on cellular cAMP levels in gastric adenocarcinoma cells(SGC-7901). Radioimmunoassay was used. The results showed that hCG obviously increased cAMP levels in SGC-7901 cells in a dose-dependent and time-dependent manner. When the concentration of hCG increased from 10(-11) mol/L to 10(-6) mol/L, the cellular cAMP levels increased from about 2.1 pmol/mg protein to about 10 pmol/mg protein and were significantly different from the control(P < 0.01). These results suggest that hCG possibly exerts its effects on gastric cancer cells by activating adenylyl cyclase/cAMP signal transduction pathway.
Zhang
Q
Q
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
Chen
M
M
D000230:Adenocarcinoma / Q000378:metabolism*; D000262:Adenylyl Cyclases / Q000502:physiology; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D006801:Humans; D011863:Radioimmunoassay; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000737:chemistry
D006063:Chorionic Gonadotropin; D000242:Cyclic AMP; D000262:Adenylyl Cyclases