title: Antifungal susceptibility testing in teaching hospitals.
abstract: BACKGROUND
  An assessment of antifungal susceptibility testing (AST) has not been conducted since the introduction of the National Committee for Clinical Laboratory Standards (NCCLS) M27-A document.
  
  
  OBJECTIVE
  To determine AST practices in teaching hospitals.
  
  
  METHODS
  A questionnaire was mailed to the heads of 386 randomly assigned microbiology departments from teaching hospitals identified through the 2000 American Hospital Association Guide. Identifiers were used to delineate responders from nonresponders. A reminder letter was mailed 3 weeks after the initial mailing to all nonresponders. The hospital bed-size and number of inpatient days for respondents were obtained through the American Hospital Directory.
  
  
  RESULTS
  The questionnaire was returned by 171 (44.3%) institutions. The total and median (range) number of candida isolates were 137,088 and 8.5 (1-145)/1000 inpatient days for the year 2000, respectively. Approximately 1% (1300) of candida isolates, from predominantly blood specimens, underwent AST. AST was reported by 115 (67.2%) hospitals, with testing on site at 27 hospitals and off site for 88 hospitals. NCCLS methodology (80% broth microdilution) was used by 75% of the hospitals performing on-site AST. The median time to obtain AST results was significantly lower when testing was performed on site (3 d) compared with off site (7-10 d).
  
  
  CONCLUSIONS
  A large number of candida bloodstream isolates undergoes AST annually. AST results are obtained sooner when performed on site compared with off site.
authors.affiliation: College of Pharmacy, University of New Mexico, Albuquerque, NM, USA.
authors.forename: Manjunath P
authors.initials: MP
authors.lastname: Pai
authors.forename: Susan L
authors.initials: SL
authors.lastname: Pendland
mesh_terms: D002175:Candida / Q000187:drug effects* / Q000302:isolation & purification; D019411:Clinical Laboratory Techniques / Q000706:statistics & numerical data*; D006784:Hospitals, Teaching / Q000706:statistics & numerical data*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000706:statistics & numerical data; D011795:Surveys and Questionnaires; D014481:United States