Molecular identification of Paracoccidioides brasiliensis by 5' nuclease assay.
A PCR assay based on the 5' nuclease assay using a fluorescent probe derived from the sequence of the gene coding for the 43,000-Da (gp43) antigen was developed to detect Paracoccidioides brasiliensis. The assay could detect at least 10 copies of this DNA sequence, providing efficient accuracy to be useful for diagnosis of paracoccidioidomycosis.
Semighini
Camile P
CP
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil.
de Camargo
Zoilo P
ZP
Puccia
Rosana
R
Goldman
Maria Helena S
MH
Goldman
Gustavo H
GH
D000951:Antigens, Neoplasm / Q000235:genetics; D015342:DNA Probes; D005092:Exonucleases / Q000378:metabolism*; D005453:Fluorescence; D005800:Genes, Fungal / Q000235:genetics; D016533:Mycological Typing Techniques / Q000379:methods*; D010228:Paracoccidioides / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*
D000951:Antigens, Neoplasm; D015342:DNA Probes; C107095:tumor-associated antigen, gp43; D005092:Exonucleases