Nucleotide profile of mouse liver: response to 2'-O-dibutyryl cytidene 3',5'-cyclic monophosphate.
The effect of 2'-O-dibutyryl cytidene 3',5'-cyclic monophosphate (dibutyryl cCMP) on the nucleotide profile of mouse liver was examined. Dibutyryl cCMP caused an increase in the amount of CTP in mouse liver. Perchloric acid extracts of liver tissue were neutralized with tri-N-octylamine in trichlorotriflouroethene and, after removal of CLO(4-), subjected to preliminary purification on a Cu2+-loaded column of Chelex 100. A high-pressure liquid chromatographic anion-exchange procedure was used and gave good resolution of the free nucleotides on a single column.
Haji Hosseini Baghdad Abadi
Reza
R
Department of Biology, Faculty of Science, Payame Noor University, Nakhel Street, Lashkarak Road, Tehran 19569, Iran. hosseini@pnu.ac.ir
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003563:Cyclic CMP / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008110:Liver Extracts / Q000378:metabolism; D051379:Mice; D009711:Nucleotides / Q000032:analysis*; D013997:Time Factors
D008110:Liver Extracts; D009711:Nucleotides; D003563:Cyclic CMP; C053441:dibutyryl cyclic-3',5'-cytidine monophosphate