title: [The preparation of norA gene probe in Staphylococcus aureus].
abstract: OBJECTIVE
  To prepare norA gene probe for studies on the mechanism of resistance to fluoroquinolones mediated by norA gene in S. aureus.
  
  
  METHODS
  The Dig-labeled norA gene probe was made by PCR.
  
  
  RESULTS
  This method is simple and feasible. We have got a lot of probes in a short time. The sensitivity of norA gene probe is high; the probe is secure, easy to use and can be stored for a long time.
  
  
  CONCLUSIONS
  The probe is applicable to further studies on the mechanism of resistance to fluoroquinolones mediated by norA in S. aureus.
authors.affiliation: Department of Infectious Diseases, First Affiliated Hospital, WCUMS, Chengdu 610041, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhong
authors.forename: P
authors.initials: P
authors.lastname: Feng
authors.forename: X
authors.initials: X
authors.lastname: Fan
authors.forename: X
authors.initials: X
authors.lastname: Lü
authors.forename: B
authors.initials: B
authors.lastname: Lei
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D015342:DNA Probes / Q000096:biosynthesis*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D024841:Fluoroquinolones; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D016133:Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D015342:DNA Probes; D024841:Fluoroquinolones; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C075040:NorA protein, Staphylococcus