[Screening of acidic xylanase producing strain and studies on its enzyme production conditions].
From 150 fungal strains, the authors found 8 strains contained mainly of xylanase activity over 100 U/mL in which the No. 149 strain was the highest xylanase producer. Which tentatively identified as Aspergillas niger. The appropriate medium composition was as follows: wheat bran hemicellulose 4%; NaNO3 1%; wheat bran 1% prepared in Mandels nutritional solution without (NH4)2SO4 and urea. After cultivated in shake-flask at 28 degrees C-32 degrees C for 60 h, the activity reached the highest value of 357.2 U/mL. The optimum pH of xylanase was 4.6 and it was stable at pH3-11. The fermented broth of strain 149 contained in addition to xylanase (relative activity 100) also included amylase(1.8), mannanase(0.98), beta-xylosidase(0.94) and cellulase(0.17).
Chen
H
H
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
Zhu
J
J
Liang
G
G
Yan
Z
Z
Zhang
S
S
D001234:Aspergillus niger / Q000201:enzymology*; D003470:Culture Media; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D004795:Enzyme Stability; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D013696:Temperature
D003470:Culture Media; D043364:Endo-1,4-beta Xylanases