title: Directing the expression of a green fluorescent protein transgene in differentiated osteoblasts: comparison between rat type I collagen and rat osteocalcin promoters.
abstract: The osteocalcin (OC) and a 2.3 kb fragment of the collagen promoter (Col2.3) have been used to restrict transgenic expression of a variety of proteins to bone. Transgenic mice carrying a green fluorescent protein (GFP) gene driven by each promoter were generated. Strong GFP expression was detected in OC-GFP mice in a few osteoblastic cells lining the endosteal bone surface and in scattered osteocytes within the bone matrix in long bones from 1-day-old to 6-month-old transgenic animals. Similar findings were noted in the forming tooth in which only individual odontoblasts expressed GFP without detectable expression from the dental pulp. This limited pattern of OC-GFP-positive cells contrasts with the uniform expression in the Col2.3GFP mice in which large proportion of osteoblasts, odontoblasts, and osteocytes strongly expressed the transgene. To assess transgene expression during in vitro differentiation, marrow stromal cell and neonatal calvarial osteoblast cultures were analyzed. The activity of both transgenes was restricted to mineralized nodules but the number of positive cells was lower in the OC-GFP-derived cultures. The different temporal and spatial pattern of each transgene in vivo and in vitro reveals potential advantages and disadvantages of these two transgene models.
authors.affiliation: Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Kalajzic
authors.forename: P
authors.initials: P
authors.lastname: Liu
authors.forename: I
authors.initials: I
authors.lastname: Kalajzic
authors.forename: Z
authors.initials: Z
authors.lastname: Du
authors.forename: A
authors.initials: A
authors.lastname: Braut
authors.forename: M
authors.initials: M
authors.lastname: Mina
authors.forename: E
authors.initials: E
authors.lastname: Canalis
authors.forename: D W
authors.initials: DW
authors.lastname: Rowe
mesh_terms: D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000502:physiology; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D015675:Osteocalcin / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000502:physiology; D011817:Rabbits; D051381:Rats; D019076:Transgenes / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: D024042:Collagen Type I; D008164:Luminescent Proteins; D015675:Osteocalcin; D049452:Green Fluorescent Proteins