title: Sevoflurane anaesthesia does not induce the formation of sister chromatid exchanges in peripheral blood lymphocytes of children.
abstract: BACKGROUND
  Compound A, a degradation product of sevoflurane, has been demonstrated to induce sister chromatid exchanges (SCE) in Chinese hamster ovary cells in vitro as a marker for possible genotoxicity. We investigated the formation of SCE in mitogen-stimulated T-lymphocytes of 40 children undergoing sevoflurane anaesthesia for minor surgical procedures.
  
  
  METHODS
  Anaesthesia was induced by inhalation of up to 8% sevoflurane and maintained at 2.5-3% in oxygen/nitrous oxide (65/35%) at a fresh gas flow of 3 litre min(-1). Soda lime (humidity 12-15%) was used as a carbon dioxide absorbent. Blood was drawn directly before induction and after termination of anaesthesia. Twenty-five second division metaphases of mitogen-stimulated T-lymphocytes per blood sample were screened for SCE rates using standard techniques.
  
  
  RESULTS
  Average duration of anaesthesia was 49.6 (SD 24.0) min. Before anaesthesia induction, 7.93 (1.23) SCE per metaphase were determined. After sevoflurane anaesthesia [1.40 (0.77) MAC h] 7.92 (1.19) SCE per metaphase were observed. Additionally, no differences were evident between male or female children.
  
  
  CONCLUSIONS
  Short-term administration of sevoflurane anaesthesia did not induce SCE in T-lymphocytes of children. No indication for a possible genotoxic effect has been observed.
authors.affiliation: Christian-Albrechts-University Kiel, Brunswiker Strasse 10, D-24105 Kiel, Germany. tkrause@uke.uni-hamburg.de
authors.forename: T
authors.initials: T
authors.lastname: Krause
authors.forename: J
authors.initials: J
authors.lastname: Scholz
authors.forename: L
authors.initials: L
authors.lastname: Jansen
authors.forename: H
authors.initials: H
authors.lastname: Boettcher
authors.forename: C
authors.initials: C
authors.lastname: Koch
authors.forename: F
authors.initials: F
authors.lastname: Wappler
authors.forename: J
authors.initials: J
authors.lastname: Schulte am Esch
mesh_terms: D000293:Adolescent; D018685:Anesthetics, Inhalation / Q000009:adverse effects*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008677:Metaphase / Q000235:genetics; D008738:Methyl Ethers / Q000009:adverse effects*; D008934:Mitogens; D000077149:Sevoflurane; D012854:Sister Chromatid Exchange; D013601:T-Lymphocytes / Q000187:drug effects* / Q000502:physiology
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D008934:Mitogens; D000077149:Sevoflurane