[Separation of different phosphatidyl cholines by reversed-phase ion-pair high performance liquid chromatography].
A reversed-phase ion-pair high performance liquid chromatographic (RP-IP-HPLC) method for the separation of different phosphatidyl cholines (PC) was established. The optimized conditions were a PERKIN-ELMER/HS-5 C18 column (150 mm x 4.6 mm i.d., 5 microns), isocratic elution with mobile phase of methanol-acetonitrile-water (70:22:8, volume ratio) containing 15 mmol/L tetramethyl ammonium phosphate (TMAP) at pH 7.0, flow rate of 2.0 mL/min and UV detection at 208 nm. Seven kinds of PC could be separated with this method.
Wang
Zhi-hua
ZH
Shanghai University of Traditional Chinese Medicine and Pharmacology, Shanghai 200032, China.
Lu
Xue-qing
XQ
Hong
Xiao-kun
XK
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D010713:Phosphatidylcholines / Q000302:isolation & purification*
D010713:Phosphatidylcholines