Efficiency and cytotoxicity of resin-based desensitizing agents.
OBJECTIVE
To compare in vitro the efficacy of five resin-based desensitizing agents at reducing human dentin permeability and to compare their cytotoxicity. The tested hypothesis was that their different curing techniques cause variations in efficiency and cytotoxicity.


METHODS
Dentin slices (0.5 +/- 0.05 mm thick) were prepared from human third molars (10 per group) and their hydraulic conductance was recorded before and after application of one of the desensitizing agents with a Flodec device. Six desensitizing agents were studied: one light curing agent (Seal and Protect); one self-curing agent (Pain Free); the resin-based agents without any polymerization initiator (Health-Dent, Gluma Desensitizer, Isodan); one oxalate-based agent served as a control (Protect). A MTT assay on L 929 fibroblasts was performed to measure the cytotoxicity of the six desensitizing agents applied onto additional dentin slices (10 per group).


RESULTS
All the desensitizing agents resulted in a large decrease in dentin permeability. The best results were obtained with Gluma Desensitizer, Isodan, Pain Free and Protect. A statistically significant difference was found among the materials (P = 0.001). All the materials were non-cytotoxic. Cell viability ranged from 88% for Seal and Protect to 100% for Isodan. No difference was found among their cytotoxicity.
Camps
Jean
J
Laboratoire IMEB, UFR Odontologie, Marseille, France. jcamps2035@aol.com
About
Imad
I
Van Meerbeek
Bart
B
Franquin
Jean Claude
JC
D000704:Analysis of Variance; D000818:Animals; D001548:Benzalkonium Compounds / Q000627:therapeutic use / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D003806:Dentin Permeability / Q000187:drug effects*; D003807:Dentin Sensitivity / Q000188:drug therapy*; D019234:Dentinal Fluid / Q000502:physiology; D005347:Fibroblasts / Q000187:drug effects; D005976:Glutaral / Q000627:therapeutic use / Q000633:toxicity; D006801:Humans; D006874:Hydrostatic Pressure; D007739:L Cells; D008689:Methacrylates / Q000627:therapeutic use / Q000633:toxicity*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009281:Naphthalenes / Q000627:therapeutic use / Q000633:toxicity; D009566:Nitrates / Q000633:toxicity; D010070:Oxalates / Q000627:therapeutic use / Q000633:toxicity; D019279:Resin Cements / Q000627:therapeutic use / Q000633:toxicity; D012969:Sodium Fluoride / Q000633:toxicity; D013499:Surface Properties
D001548:Benzalkonium Compounds; C473099:Isodan; D008689:Methacrylates; D009281:Naphthalenes; D009566:Nitrates; D010070:Oxalates; C121034:Pain-Free; D019279:Resin Cements; C442646:Seal and Protect resin cement; C111486:gluma desensitizer; C111487:health-dent desensitizer; C012514:1,8-naphthalenedicarboxylic acid anhydride; D012969:Sodium Fluoride; D005976:Glutaral