Magnetic resonance imaging and neurosphere therapy of stroke in rat.
We intracisternally transplanted subventricular zone (SVZ) cells labeled by ferromagnetic particles into stroked rats. Migration of transplanted cells was non-invasively tracked using magnetic resonance imaging (MRI). We found that transplanted cells selectively migrated towards the ischemic parenchyma at a mean speed of 65 +/- 14.6 microm/hr in living rats. Migration of transplanted cells in the brain was also measured histopathologically. Rats transplanted with SVZ cells exhibited significant improvement of neurological function. Our data suggest that intracisternal transplantation of SVZ cells provides an avenue for cell therapy of stroke and that MRI can be used to track grafted cells in the brain.
Zhang
Zheng Gang
ZG
Department of Neurology, Henry Ford Health Sciences Center, Detroit, MI 48202, USA.
Jiang
Quan
Q
Zhang
Ruilan
R
Zhang
Li
L
Wang
Lei
L
Zhang
Lijei
L
Arniego
Polly
P
Ho
Khang-Loon
KL
Chopp
Michael
M
D000818:Animals; D002545:Brain Ischemia / Q000473:pathology / Q000601:surgery; D002465:Cell Movement; D008279:Magnetic Resonance Imaging; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology; D020521:Stroke / Q000473:pathology* / Q000601:surgery*