[Effect of calcium on adherence of Streptococcus mutans MT6R(serotype c) surface protein P1].
OBJECTIVE
To study effects of calcium on the adherence of Streptococcus mutans MT6R(serotype c) surface protein P1 to saliva-coated hydroxyapatite(S-HA).


METHODS
The surface protein P1 of Streptococcus mutans MT6R was purified by PAGE and labeled with 131I(131I-P1). The adherence amount of 131I-P1 to S-HA in different calcium concentration liquid was measured.


RESULTS
The amount of 131I-P1 adherence to S-HA in 0, 0.1, 0.25, 0.5, 1.0, 1.5, 2.0 mmol/L, and calcium was 7175 + 183, 7516 + 192, 8850 + 215, 9335 + 204, 10087 + 228, 10179 + 224 and 10200 + 317 cpm, respectively(P < 0.01). The calcium concentration from 0 to 1.0 mmol/L, the amount of 131I-P1 adherence was increased statistically(P < 0.05), calcium concentration from 1.0 mmol/L to 2.0 mmol/L, the amount of 131I-P1 adherence was increased unstatistically(P > 0.05).


CONCLUSIONS
Calcium promoted the adherence of surface protein P1 to the S-HA. This result suggested that calcium was involved in the adherence of Streptococcus mutans MT6R (serotype c) surface protein P1.
Huang
D
D
College of Stomatology, West China University of Medical Sciences.
Luo
Z
Z
Zhou
X
X
D001422:Bacterial Adhesion / Q000187:drug effects*; D001426:Bacterial Proteins / Q000502:physiology; D002118:Calcium / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008565:Membrane Proteins / Q000502:physiology*; D013295:Streptococcus mutans / Q000737:chemistry*
D001426:Bacterial Proteins; D008565:Membrane Proteins; D002118:Calcium