title: Epilepsy-induced changes in signaling systems of human and rat postsynaptic densities.
abstract: OBJECTIVE
  To study seizure-induced changes in signaling proteins present in postsynaptic densities (PSDs) isolated from human epileptic neocortex and from rat cortex in which seizures were induced by injection of kainic acid.
  
  
  METHODS
  We performed Western blot analysis of signaling proteins in PSDs isolated from cortical tissue.
  
  
  RESULTS
  Seizures induce a strong upregulation of TrkB, the receptor for brain-derived neurotrophic factor (BDNF), whereas components of the N-methyl-d-aspartate (NMDA)-receptor complex are downregulated in both human and rat PSDs.
  
  
  CONCLUSIONS
  These data show that long-term changes in PSD composition occur as a consequence of epileptic seizure activity.
authors.affiliation: Laboratorio de Neurociencias, Universidad de los , Santiago, Chile. uwyneken@uandes.cl
authors.forename: Ursula
authors.initials: U
authors.lastname: Wyneken
authors.forename: Juan-Jose
authors.initials: JJ
authors.lastname: Marengo
authors.forename: Sergio
authors.initials: S
authors.lastname: Villanueva
authors.forename: Dagoberto
authors.initials: D
authors.lastname: Soto
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Sandoval
authors.forename: Eckart D
authors.initials: ED
authors.lastname: Gundelfinger
authors.forename: Fernando
authors.initials: F
authors.lastname: Orrego
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000503:physiopathology*; D002648:Child; D002675:Child, Preschool; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D004827:Epilepsy / Q000139:chemically induced / Q000503:physiopathology*; D005260:Female; D006801:Humans; D007223:Infant; D007608:Kainic Acid; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D020813:Receptor, trkB / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D020813:Receptor, trkB; D007608:Kainic Acid