title: Characterization of a rhamnogalacturonan and a xyloglucan from neriumindicum and their activities on PC12 pheochromocytoma cells.
abstract: Two polysaccharides, a rhamnogalacturonan (J1) and a xyloglucan (J2), were isolated and purified from the whole flowers of Nerium indicum and characterized by GC, GC-MS, NMR, and ESMS. The rhamnogalacturonan was found to consist of repeating units of a linear trisaccharide with the structure [-->4)-alpha-GalpA(1-->2)alpha-Rhap(1-->4)-alpha-GalpA(1-->4)-](n)(), while the xyloglucan consisted of repeating units of the trisaccharide, which was (1-->4)-linked beta-glucopyranosyl residues as a backbone and substituted at position 6 linked to terminal alpha-xylopyranose side chains. These two polysaccharides were tested on the proliferation and differentiation on PC12 pheochromocytoma cells and found to have effects similar to nerve growth factor (NGF).
authors.affiliation: Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, People's Republic of China.
authors.forename: Kan
authors.initials: K
authors.lastname: Ding
authors.forename: Ji-Nian
authors.initials: JN
authors.lastname: Fang
authors.forename: Tingxia
authors.initials: T
authors.lastname: Dong
authors.forename: Karl Wati-Keung
authors.initials: KW
authors.lastname: Tsim
authors.forename: Houming
authors.initials: H
authors.lastname: Wu
mesh_terms: D000818:Animals; D002681:China; D004578:Electron Spin Resonance Spectroscopy; D035264:Flowers / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D005936:Glucans; D030007:Nerium / Q000737:chemistry*; D020932:Nerve Growth Factor / Q000187:drug effects; D019906:Nuclear Magnetic Resonance, Biomolecular; D016716:PC12 Cells / Q000187:drug effects; D010368:Pectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010673:Pheochromocytoma; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D014990:Xylans
chemical_list: D005936:Glucans; D011134:Polysaccharides; D014990:Xylans; C473998:rhamnogalacturonan J1; C029353:xyloglucan; D010368:Pectins; D020932:Nerve Growth Factor