Comparative study on the characterization of antisera of anti-aflatoxin B1 from rabbit and laying hen.
Polyclonal antisera specific for aflatoxin B1 (AFB1) were developed in rabbits and laying hens immunized with AFB1 conjugated to bovine serum albumin (AFB1-BSA). During the immunization schedule, AFB1-specific antisera from rabbits started to obviously be secreted after 60 days of the first AFB1-BSA injection, reached to a peak at day 120, and started to go down at day 135; while in regard to laying hens, antibodies began to largely be produced at day 90, arrived to a peak at day 135, and began to go down at day 165. The rabbits consistently produced anti-AFB1 antisera in 2-fold higher amounts than the laying hens during the antisera production process. But rabbits' and laying hens' antisera to AFB1 were found not to be apparently different in cross-reaction with aflatoxin analogs. Both rabbits' and laying hens' antisera could be used as immunological reagents for detecting AFB1 in agricultural commodities.
Chen
Fusheng
F
Key Laboratory of Agro-Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, P. R. China. chenfs@027.net
Zhou
Qi
Q
D016604:Aflatoxin B1 / Q000032:analysis / Q000276:immunology*; D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology*; D000917:Antibody Formation; D001230:Aspergillus / Q000276:immunology; D002645:Chickens; D018556:Crops, Agricultural / Q000737:chemistry / Q000382:microbiology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005506:Food Contamination / Q000032:analysis; D007106:Immune Sera / Q000276:immunology*; D007114:Immunization / Q000662:veterinary; D011817:Rabbits
D000908:Antibodies, Fungal; D007106:Immune Sera; D016604:Aflatoxin B1