[Role of N-methyl-D-aspartat receptor-nitric oxide-cyclic guanosin monophosphate pathway in ischemic brain injury].
OBJECTIVE
To observe the role of NMDA receptor-Nitric Oxide(NOS)-cGMP pathway in ischemic brain injury.


METHODS
The common cervical arteries of the rats were transient blocked bilaterally, in association with bleeding from their tails and followed by reperfusion. The procedures were repeated once again as above to establish a stable ischemic brain injury model. 3H-MK801 binding, cNOS activity, iNOS activity, and the cGMP content were measured at different time intervals after ischemic injury.


RESULTS
The results indicated that the change of 3H-MK801 binding varied among the cerebral cortex and hippocampus. cNOS activity began to rise in all parts of the brain 24 hours after operation and reached its peak in 3 days. The regions where iNOS and cNOS activity and cGMP content all increased significantly include the hippocamus, striatum and cortex, especially in hippocampus.


CONCLUSIONS
The tendency and degree of increase of those four indixes were cosistent during ischemic injury, indicating an important role of NMDA receptor-NO-cGMP in ischemic injury of the hippocampus.
Qiang
W
W
Department of Pharmacology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Zuo
P
P
Liu
J
J
D000818:Animals; D001921:Brain / Q000378:metabolism; D002545:Brain Ischemia / Q000150:complications; D006152:Cyclic GMP / Q000378:metabolism*; D005260:Female; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism*
D016194:Receptors, N-Methyl-D-Aspartate; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; C496322:Nos1 protein, rat; C496318:Nos2 protein, rat; D006152:Cyclic GMP