[Excretion ratio of lactulose and mannitol as intestinal permeability index in healthy Chinese volunteers].
OBJECTIVE
To determine lactulose (L)/mannitol (M) excretion ratio with high pressure liquid chromatograph with pulsed electrochemical detection (HPLC-PED) in healthy Chinese volunteers.


METHODS
Sixty healthy Chinese volunteers enrolled. For each volunteer the L/M permeability study was performed twice by two different doses, as small dose (1 g mannitol and 2 g lactulose) and regular dose (5 g mannitol and 10 g lactulose). The urine were collected during 6 hours after ingestion of the test solution. The M and L concentrations were analyzed by HPLC with pulsed electrochemical detection.


RESULTS
The L/M ratio was 0.026 +/- 0.006 in the small doses tests, and 0.036 +/- 0.008 in the large doses tests.


CONCLUSIONS
With HPLC-PED detection, a low doses of lactulose and mannitol can be used to study intestinal permeability. Determination of the L/M ratio may be a way of evaluating intestinal barrier function in clinical practice.
Liu
W
W
Department of Surgery, PUMC Hospital, CAMS and PUMC, Beijing 100730.
Jiang
Z
Z
Shu
H
H
Sun
M
M
Li
D
D
D000328:Adult; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007792:Lactulose / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000652:urine*; D008297:Male; D008353:Mannitol / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000652:urine*; D008875:Middle Aged; D010539:Permeability; D012016:Reference Values
D008353:Mannitol; D007792:Lactulose