Coupled liquid chromatography-gas chromatography for the rapid analysis of gamma-oryzanol in rice lipids.
An approach based on on-line coupled liquid chromatography-gas chromatography (LC-GC) was developed for the rapid analysis of gamma-oryzanol in rice. Total lipids were extracted from rice and subjected to LC-GC without any prior purification. gamma-Oryzanol was pre-separated by HPLC from rice lipids and transferred on-line to GC analysis in order to separate its major constituents. 24-methylenecycloartanyl ferulate, cycloartenyl ferulate, campesteryl ferulate, beta-sitosteryl ferulate and campestanyl ferulate. The identities of the compounds were confirmed by off-line GC-MS analysis. Total gamma-oryzanol content could be quantified by HPLC-UV detection and the distribution of gamma-oryzanol constituents could be determined by on-line coupled GC analysis. The proposed methodology paves the way for high-throughput investigations providing information on natural variations in gamma-oryzanol content and its composition in different rice varieties.
Miller
Andreas
A
Lehrstuhl für Allgemeine Lebensmitteltechnologie, Technische Universität München, Am Forum 2, D-85350 Freising-Weihenstephan, Germany.
Frenzel
Thomas
T
Schmarr
Hans-Georg
HG
Engel
Karl-Heinz
KH
D002849:Chromatography, Gas / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008055:Lipids / Q000737:chemistry*; D012275:Oryza / Q000737:chemistry*; D010666:Phenylpropionates / Q000032:analysis*
D008055:Lipids; D010666:Phenylpropionates; C013172:gamma-oryzanol