title: The separation of biomolecules using capillary electrochromatography.
abstract: The unique properties of capillary electrochromatography such as high performance, high selectivity, minimum consumption of both reagents and samples, and good compatibility with mass spectrometry make this technique an attractive one for the analysis of biomolecules including peptides, proteins, carbohydrates, nucleosides and nucleotides. Irreversible adsorption between the biomolecules and the charged packing surface leads to a lack of reproducibility and serious peak tailing, so various approaches have been taken to overcome this and to improve the technique for future challenges.
authors.affiliation: National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China.
authors.forename: Hongjing
authors.initials: H
authors.lastname: Fu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Huang
authors.forename: Wenhai
authors.initials: W
authors.lastname: Jin
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
mesh_terms: D001704:Biopolymers / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D009705:Nucleosides / Q000032:analysis / Q000302:isolation & purification; D009711:Nucleotides / Q000032:analysis / Q000302:isolation & purification; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification*; D011786:Quality Control; D012680:Sensitivity and Specificity
chemical_list: D001704:Biopolymers; D009705:Nucleosides; D009711:Nucleotides; D010455:Peptides; D011506:Proteins