[A simple method for estimation of concentration of vitamins E and A in low-density lipoproteins].
The aim of the study is to work out a new easy method for determination of fat-soluble E and A vitamins concentration in low density lipoproteins (LDL). The method was worked out in the blood serum of 60 healthy men aged 35-55 with monolipidaemia and 55 men aged 45-65 with dislipoproteinemia (DLP). The new method consists of LDL isolation from serum by heparin precipitation method and determination of alpha-tocopherol and retinol concentrations by spectro-fluorimetrical method. The new method is informative for estimation of LDL antioxidant system, easy and fast to perform. The new method features a high reproduction and strong positive correlation between levels of E and A vitamins in the blood serum by spectro-fluorimetrical method and levels of E and A vitamins in LDL by our method. The use of both methods shows reliable reduction in E and A vitamins concentration in the blood serum and in LDL in men with DLP type II compared with E and A vitamins concentration in men with normolipidaemia.
Ragino
Iu I
IuI
Kashtanova
E V
EV
D000328:Adult; D000368:Aged; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D014801:Vitamin A / Q000097:blood*; D014810:Vitamin E / Q000097:blood*
D008077:Lipoproteins, LDL; D014801:Vitamin A; D014810:Vitamin E