Protective effects of Wuyaoshunqisan against H2O2-induced apoptosis on hippocampal cell line HiB5.
Oxidative stress has been implicated in the pathogenesis of different neurodegenerative disorders. To investigate the protective effects of Wuyaoshunqisan against H2O2-induced apoptosis in the central nervous system, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) method, flow cytometric analysis, and the DNA fragmentation assay were performed on cells of the hippocampal cell line HiB5. Through the morphological and biochemical analyses, it was shown that HiB5 cells treated with H2O2 exhibit classical apoptotic features, while the occurrence of such changes is reduced in cells pre-treated with Wuyaoshunqisan prior to H2O2 exposure.
Je
Jeong-Jin
JJ
Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyung Hee University, Seoul, Korea.
Shin
Hyun-Taeg
HT
Chung
Seok-Hee
SH
Lee
Jong-Soo
JS
Kim
Sung-Soo
SS
Shin
Hyun-Dae
HD
Jang
Mi-Hyeon
MH
Kim
Youn-Jung
YJ
Chung
Joo-Ho
JH
Kim
Ee-Hwa
EH
Kim
Chang-Ju
CJ
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D005434:Flow Cytometry; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D020287:In Situ Nick-End Labeling; D007202:Indicators and Reagents; D016877:Oxidants / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013778:Tetrazolium Salts; D013844:Thiazoles
D000975:Antioxidants; D007202:Indicators and Reagents; D016877:Oxidants; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; C473660:wuyaoshunqisan; D006861:Hydrogen Peroxide; C022616:thiazolyl blue