title: The Cu,Zn superoxide dismutase in neuroblastoma SK-N-BE cells is exported by a microvesicles dependent pathway.
abstract: The antioxidant enzyme Cu,Zn superoxide dismutase has so far been considered costitutively expressed and exclusively localized into cytosol. In this paper we investigated Cu,Zn superoxide dismutase export in neuroblastoma SK-N-BE cells by flow cytometry analysis, confocal immunofluorescence analysis and enzyme-linked immunosorbed assay. Immunofluorescence analysis shows that the enzyme is exported by microvesicular granules; moreover the treatment of cells with brefeldin A and with 2-deoxy-D-glucose and sodium azide strongly decreases the amount of CuZn superoxide dismutase detected in the medium. Therefore the involvement of ATP-dependent mechanisms, likely including BFA-sensitive intracytoplasmic vesicles in Cu,Zn SOD export from SK-N-BE cells, has to be hypothesized. Microvesicular-mediated Cu,Zn SOD export in neurons could represent a relevant phenomenon able to influence cell excitability that is affected by reactive oxygen species.
authors.affiliation: Department of Neuroscience and Behavioral Sciences, Section of Physiology, University of Naples Federico II, Via S. Pansini 5, 80131 Naples, Italy. mondola@unina.it
authors.forename: Paolo
authors.initials: P
authors.lastname: Mondola
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Ruggiero
authors.forename: Rosalba
authors.initials: R
authors.lastname: Serù
authors.forename: Simona
authors.initials: S
authors.lastname: Damiano
authors.forename: Serena
authors.initials: S
authors.lastname: Grimaldi
authors.forename: Corrado
authors.initials: C
authors.lastname: Garbi
authors.forename: Marcellino
authors.initials: M
authors.lastname: Monda
authors.forename: Dario
authors.initials: D
authors.lastname: Greco
authors.forename: Mariarosaria
authors.initials: M
authors.lastname: Santillo
mesh_terms: D015153:Blotting, Western; D020126:Brefeldin A / Q000494:pharmacology; D022162:Cytoplasmic Vesicles / Q000502:physiology*; D003599:Cytoskeleton / Q000378:metabolism; D003847:Deoxyglucose / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D008744:Methylamines / Q000494:pharmacology; D009447:Neuroblastoma; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D021381:Protein Transport / Q000187:drug effects / Q000502:physiology*; D019810:Sodium Azide / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D004791:Enzyme Inhibitors; D008744:Methylamines; D011500:Protein Synthesis Inhibitors; D020126:Brefeldin A; D019810:Sodium Azide; D003847:Deoxyglucose; C027451:methylamine; D013482:Superoxide Dismutase