title: Enantioselective binding of lambda- and delta-[Ru(bpy)(2)(HPIP)]Cl2 (HPIP=2-(2-hydroxyphenyl)imidazo[4,5-f][1,10]phenanthroline) to the hexanucleotide [d(5'-GTCGAC-3')2].
abstract: Multidimensional NMR techniques (1D (1)HNMR, 2D DQF (1)H(1)H COSY and 2D (1)H(1)H NOESY), electrospray ionization mass spectrometry (ESI-MS) and electronic spectroscopy, were performed to study the interactions of the enantiomers lambda- and delta-[Ru(bpy)(2)(HPIP)]Cl(2), (HPIP=2-(2-hydroxyphenyl)imidazo[4,5-f][1,10]phenanthro-line) with the self complementary hexanucleotide duplex d(5'-GTCGAC-3')(2). The results show that the delta-[Ru(bpy)(2)(HPIP)]Cl(2) binds tightly to the oligonucleotide, by intercalation of the ligand HPIP, between the A5 and C6 base sequence of the same strand, probably through the minor groove. Lambda-enantiomer binds weakly, suggesting groove interactions with the hexanucleotide duplex. ESI-MS spectrometry and UV-vis spectroscopy also confirmed these observations.
authors.affiliation: Laboratory of Inorganic and General Chemistry, Department of Chemistry, University of Ioannnina, 45110 Ioannina, Greece. agaroufis@cc.uoi.gr
authors.forename: Achilleas
authors.initials: A
authors.lastname: Garoufis
authors.forename: Jin Gang
authors.initials: JG
authors.lastname: Liu
authors.forename: Liang Nian
authors.initials: LN
authors.lastname: Ji
authors.forename: Nick
authors.initials: N
authors.lastname: Hadjiliadis
mesh_terms: D001665:Binding Sites; D007364:Intercalating Agents / Q000737:chemistry; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013057:Spectrum Analysis; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
chemical_list: D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; D009942:Organometallic Compounds; D010618:Phenanthrolines; C474863:ruthenium(bipyridyl)(2)-2-(2-hydroxyphenyl)imidazo(4,5-f)(1,10)phenanthroline; D012428:Ruthenium