title: Effect of broiler age, feed withdrawal, and transportation on levels of coliforms, Campylobacter, Escherichia coli and Salmonella on carcasses before and after immersion chilling.
abstract: A study was conducted to determine effects of bird age at slaughter, feed withdrawal, and transportation on levels of coliforms, Campylobacter, Escherichia coli, and Salmonella on carcasses before and after immersion chilling. Broilers were processed at 42, 49, and 56 d of age after a 12-h feed withdrawal period or a 0-h feed withdrawal period (full fed). At each age, broilers were processed from two commercial farms previously identified as Campylobacter positive. One week before slaughter, broilers were gavaged with nalidixic acid-resistant Salmonella. During bleeding, cotton plugs were inserted into the cloaca of each carcass. Whole-carcass rinses (WCR) were performed before and after immersion chilling with 20 ppm sodium hypochlorite, and rinses were analyzed for coliforms, Campylobacter, E. coli and Salmonella. Log10 counts for coliforms, Campylobacter, and E. coli were (P < 0.05) affected by bird age at slaughter. Feed withdrawal (FW) affected only Campylobacter on carcasses of older broilers (56 d of age). Chilling with sodium hypochlorite resulted in log10 reductions of 1.2, 1.3, 1.4, and 0.5 for coliforms, Campylobacter, E. coli, and Salmonella, respectively. Under the conditions of this experiment, it appears that contamination on the exterior of birds entering the processing facility is critical to carcass bacterial counts. Moreover, carcass bacterial counts did not vary when microbial counts of broilers were comparable. FW may increase prechill carcass counts for E. coli and Campylobacter, but it appears to have no effect on postchill carcass counts when sodium hypochlorite is used in the chilling operation.
authors.affiliation: USDA Agricultural Research Service, Russell Research Center, PO Box 5677, Athens, Georgia 30604-5677, USA. jnorthcutt@saa.ars.usda.gov
authors.forename: J K
authors.initials: JK
authors.lastname: Northcutt
authors.forename: M E
authors.initials: ME
authors.lastname: Berrang
authors.forename: J A
authors.initials: JA
authors.lastname: Dickens
authors.forename: D L
authors.initials: DL
authors.lastname: Fletcher
authors.forename: N A
authors.initials: NA
authors.lastname: Cox
mesh_terms: D000375:Aging; D000818:Animals; D002167:Campylobacter / Q000302:isolation & purification; D002645:Chickens / Q000382:microbiology / Q000502:physiology*; D003080:Cold Temperature; D015169:Colony Count, Microbial; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D004926:Escherichia coli / Q000302:isolation & purification; D005508:Food Deprivation; D008460:Meat / Q000382:microbiology*; D012475:Salmonella / Q000302:isolation & purification; D014186:Transportation