Single-cell kinase assays: opening a window onto cell behavior.
Recent advances in analytical techniques have made the performance of biochemical assays on individual mammalian cells possible. Of particular interest is the ability to measure the activation of kinases, enzymes with critical roles in virtually every aspect of cell physiology. Single-cell kinase assays promise to deliver a newfound understanding of the molecular mechanisms responsible for cellular control and behavior by revealing the dynamic nature of signal transduction networks in living cells. A recent exciting development is the potential to perform assays of multiple kinases simultaneously in a single cell.
Sims
Christopher E
CE
Department of Physiology and Biophysics, D380 Medical Sciences, University of California, Irvine, CA 92697-4560, USA.
Allbritton
Nancy L
NL
D002477:Cells / Q000201:enzymology; D004586:Electrophoresis / Q000379:methods; D004789:Enzyme Activation; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010770:Phosphotransferases / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods; D013379:Substrate Specificity
D011994:Recombinant Proteins; D010770:Phosphotransferases