Thermoradiotherapy combined with adenoviral p53 gene therapy in head and neck squamous cell carcinoma cell lines.
We examined effects of recombinant p53-expressing adenovirus combined with thermoradiotherapy in 8 head and neck squamous cell carcinoma (SCC) cell lines to improve the outcomes of the treatment of advanced head and neck cancer. The p53 gene therapy did not improve the discrepancy between thermoradiosensitivities among the 8 SCC cell lines. However, p53 gene therapy improved the effects of thermoradiotherapy in all 8 cell lines, and there were significant differences in four situations of the HSC4 44 degrees C (p=0.032), SAS at 44 degrees C (p=0.029), the KB at 43 degrees C (p=0.025), and the Ca9-22 43 degrees C (p=0.020). In comparing the survival rates of thermoradiotherapy with those of thermotherapy and radiotherapy, thermoradiotherapy demonstrated actual survival rates less than theoretical survival rates based on the survival rates of thermotherapy multiplied by the survival rates of radiotherapy in almost all treatments of thermoradio-gene therapy of the 8 SCC cell lines. These results demonstrate that thermoradiotherapy combined with p53 gene therapy may be a useful tool in treating SCC cells.
Higuchi
Yuzuru
Y
Department of Oral and Maxillofacial Radiology, Graduate School of Medicine and Dentistry, Okayama University, Okayama, Japan.
Asaumi
Jun-ichi
J
Murakami
Jun
J
Matsuzaki
Hidenobu
H
Wakasa
Toru
T
Inoue
Tetsuyoshi
T
Konoucni
Hironobu
H
Hisatomi
Miki
M
Yanagi
Yoshinobu
Y
Honda
Yasutoshi
Y
Shigehara
Hiroshi
H
Kishi
Kanji
K
D000256:Adenoviridae / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000628:therapy*; D002470:Cell Survival; D003131:Combined Modality Therapy; D004307:Dose-Response Relationship, Radiation; D016158:Genes, p53; D015316:Genetic Therapy; D006258:Head and Neck Neoplasms / Q000378:metabolism / Q000628:therapy*; D006801:Humans; D006979:Hyperthermia, Induced; D018714:Radiotherapy, Adjuvant; D013696:Temperature; D013997:Time Factors; D014162:Transfection; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000378:metabolism
D001616:beta-Galactosidase