[Clinical significance of detecting telomerase activity in ascites cells from patients with ovarian carcinoma].
OBJECTIVE
The purpose was to investigate the clinical significance of telomerase activity for tumor diagnosis in ascites cells from patients with ovarian carcinoma.


METHODS
Thirty ascites specimens from patients with ovarian tumor were examined for telomerase activity by PCR-based TRAP (telomeric repeat amplification protocol) method, and the result of detection was compared with the cytological diagnosis.


RESULTS
Telomerase activity was detected in ascites cells from patients with ovarian tumor in 19 of the 20 cytologic-possitive specimens and in 3 of the 7 cytologic-negative specimens and in all the 3 cytologic-suspicious specimens. The positive rate in 30 ascites samples by cytological examination and by the detection of telomerase activity was 66.67% (20/30), and 83.33% (25/30), respectively.


CONCLUSIONS
The result suggested that measuring telomerase activity in ascites cells would be helpful to cytological examination in diagnosis of patients with ovarian carcinoma.
Jin
S
S
Detection Center of Cancer Biology, Cancer Institute, Cancer Hospital, CAMS and PUMC, Beijing 100021.
Zhang
W
W
Wang
H
H
Liu
Y
Y
Gao
F
F
D001201:Ascites / Q000201:enzymology*; D018282:Cystadenocarcinoma, Mucinous / Q000175:diagnosis / Q000473:pathology; D018284:Cystadenocarcinoma, Serous / Q000175:diagnosis* / Q000473:pathology; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods; D019098:Telomerase / Q000378:metabolism*
D019098:Telomerase