Fluorescence spectral properties of cyanine dye labeled DNA near metallic silver particles.
Recent studies have demonstrated that silver metallic particles can increase the quantum yield and decrease the lifetimes of nearby fluorophores. These studies are extended to double stranded DNA oligomers labeled with N,N'-(dipropyl)-tetramethylindocarbocyanine (Cy3) or N,N-(dipropyl)-tetramethylindodicarbocyanine (Cy5). The proximity to silver particles increases the apparent quantum yields and decreases the lifetimes of the double helical DNA 23-mer labeled individually with Cy3 or Cy5. The decreased lifetimes are accompanied by apparently increased photostability of the labeled oligomers near silver particles. Because of spatial averaging across the sample these results are likely to significantly underestimate the effects of silver particles on labeled DNA localized at an optimal distance from the metallic surface. These results suggest that DNA arrays fabricated on substrates with silver particles can display increased sensitivity and photostability in the analysis of gene expression.
Malicka
Joanna
J
Department of Chemistry, University of Gdansk, 80-952 Gdansk, Sobieskiego 18, Poland.
Gryczynski
Ignacy
I
Maliwal
Badri P
BP
Fang
Jiyu
J
Lakowicz
Joseph R
JR
D001483:Base Sequence; D002232:Carbocyanines / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D005453:Fluorescence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D020411:Oligonucleotide Array Sequence Analysis; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D002232:Carbocyanines; C089187:cyanine dye 3; C085321:cyanine dye 5; D012834:Silver; D004247:DNA