Differential gene expression in retina of myopic chicken eyes using mRNA differential display.
OBJECTIVE
To study differentially expressed genes in retina of experimental myopic chicken.


METHODS
Experimental myopia in chicken was induced by form-deprivation. The mRNA in chicken retina was analyzed by using differential display.


RESULTS
Experimental myopia was successfully induced in chicken through form-deprivation. Differentially expressed gene fragments were detected in retina of chicken with myopic development and recovery as compared with normal controls.


CONCLUSIONS
The differential display of mRNA may be a useful way in cloning myopic-related genes.
Shen
H
H
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Zhang
Q
Q
Xiao
X
X
Li
S
S
Guo
X
X
Jia
X
X
Guo
L
L
D000818:Animals; D002645:Chickens; D018076:DNA, Complementary; D020869:Gene Expression Profiling / Q000379:methods; D009216:Myopia / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D012160:Retina / Q000378:metabolism*
D018076:DNA, Complementary; D012333:RNA, Messenger