[Experimental study on rabbit periosteal osteoblasts and renal vascular endothelial cells indirect co-culture in vitro].
OBJECTIVE
To determine an optimal co-culture ratio of the rabbit periosteal osteoblasts (RPOB) and rabbit renal vascular endothelial cells(RRVEC) without direct contact for future study of bone tissue engineering.


METHODS
RPOB and RRVEC in the ratios of 1:0(control group), 2:1(group 1), 1:1(group 2) and 1:2(group 3) were co-cultured by six well plates and cell inserts. Four days later, the proliferation of RPOB and RRVEC were examined through cell count. Differentiated cell function was assessed by alkaline phosphatase (ALP) activity assay and 3H proline incorporation assay.


RESULTS
When RPOB and RRVEC were indirectly co-cultured, the proliferation of RPOB and 3H proline incorporation was higher in group 1 than in the other experimental groups and control group (P < 0.05). ALP activity of RPOB was higher in group 1 than in control group and group 3 (P < 0.05), but there was no significant difference between group 1 and group 2 (P > 0.05).


CONCLUSIONS
These results suggest that RPOB and RRVEC co-cultured in a ratio of 2:1 is optimal for future study of bone tissue engineering.
Zhu
Wei-nan
WN
Key Laboratory of Transplant Engineering and Immunology, Ministry of Public Health: Institute of Reparative and Reconstructive Surgery, Lab of Tissue Engineering, West China Hospital, Sichuan University, Chengdu, Sichuan, P. R. China 610041.
Yang
Zhi-ming
ZM
Li
Xiu-qun
XQ
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D004730:Endothelium, Vascular / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology* / Q000201:enzymology; D010521:Periosteum / Q000166:cytology* / Q000201:enzymology; D011392:Proline / Q000378:metabolism; D011817:Rabbits; D023822:Tissue Engineering
D011392:Proline; D000469:Alkaline Phosphatase