[Experimental study of inhibitory effect of kangxian recipe on TGF-beta 1 induced hepatocyte apoptosis].
OBJECTIVE
To study the inhibitory effect of Kangxian Recipe (KXR) on TGF-beta 1 induced hepatocyte apoptosis.


METHODS
The in vitro model of hepatocyte apoptosis was established by cell biologic methods, utilizing the characteristics of TGF-beta 1 to observe the inhibitory effect of KXR on hepatocyte apoptosis.


RESULTS
TGF-beta 1 induced apoptosis of hepatocyte in a dose-dependent manner. The apoptosis rate of 2.2.15 cells was 63% when 500 ng/L TGF-beta 1 was used, while for HepG2 cell, it was merely 44%. After treatment of 20 ng/L KXR, the apoptosis rate of the two kinds of cell lines lowered to 33% and 24% respectively. The inhibition rate of both groups was about 50%.


CONCLUSIONS
KXR had strong inhibitory effect on hepatocyte apoptosis induced by TGF-beta 1.
Zhang
L H
LH
First Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou 310003.
Wu
J G
JG
Yao
H P
HP
D017209:Apoptosis / Q000187:drug effects*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006509:Hepatitis B / Q000473:pathology; D018197:Hepatoblastoma / Q000473:pathology; D022781:Hepatocytes / Q000473:pathology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D016212:Transforming Growth Factor beta