Synthesis and organotropism of 3H-dihydro derivatives of the cyanobacterial peptide hepatotoxin nodularin.
Tritium-labelled dihydro derivatives of the cyanobacterial peptide hepatotoxin nodularin were prepared by reduction with sodium boro[3H]hydride. The optimised reaction gave two dihydronodularin stereoisomers which were purified by high-performance liquid chromatography with a mobile phase of methanol-0.7% sodium sulfate (6:4) and a C(18) stationary phase. The specific activities of the stereoisomers were 1780-1807 dis min(-1) ng(-1). The radiolabelled dihydronodularins were tested for stability and used for toxicokinetic studies in mice. Liver was the main site of toxin accumulation.
Spoof
Lisa
L
Department of Biochemistry and Pharmacy, Abo Akademi University, P.O. Box 66, 20521, Turku, Finland.
Klimova
Svetlana
S
Mikhailov
Andrey
A
Eriksson
John E
JE
Meriluoto
Jussi
J
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria; D000087522:Cyanobacteria Toxins; D005260:Female; D007553:Isotope Labeling; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008387:Marine Toxins / Q000138:chemical synthesis* / Q000493:pharmacokinetics / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D010456:Peptides, Cyclic / Q000138:chemical synthesis* / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D013237:Stereoisomerism; D014316:Tritium / Q000737:chemistry
D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D010456:Peptides, Cyclic; C063998:nodularin; D014316:Tritium; C080847:dihydronodularin