title: Functional and Vbeta repertoire characterization of human CD8+ T-cell subsets with natural killer cell markers, CD56+ CD57- T cells, CD56+ CD57+ T cells and CD56- CD57+ T cells.
abstract: We investigated the individual CD8+ populations with natural killer (NK) cell markers (NK-type T cell); CD56 single positive (CD56)-T cells, CD56/CD57 double positive (DP)-T cells and CD57 single positive (CD57)-T cells in the peripheral blood. All NK-type T-cell populations expressed CD122 and intermediate levels of T-cell receptor (TCR; regular CD8+ T cells are CD122- and express high levels of TCR). The number of both DP-T cells and CD57-T cells, but not CD56-T cells, gradually increased with age. All NK-type T-cell populations produced larger amounts of interferon-gamma than did regular CD8+ T cells after stimulation with interleukin (IL)-2, IL-12 and IL-15. However, CD56-T cells and CD57-T cells but not DP-T cells showed a potent antitumour cytotoxity to NK-sensitive K562 cells, whereas only CD56-T cells showed a potent cytotoxity to NK-resistant Raji cells. Furthermore, although NK-type T cells produced large amounts of soluble Fas-ligands, their cytotoxic activities appeared to be mediated by the perforin/granzyme pathway. The oligoclonal or pauciclonal expansions of certain VbetaT cells were found in each NK-type T-cell population. The non-variant CDR3 region(s) for the TCRbeta chain(s) showed CD57-T cells and CD56-T cells to be derived from distinct origins, while the DP-T cell population consisted of a mixture of the clones seen in both CD56-T cells and CD57-T cells. Our results suggest that CD57-T cells and CD56-T cells are functionally and ontogenically different populations while DP-T cells appear to originate from both CD56-T cells and CD57-T cells.
authors.affiliation: Department of Parasitology, National Defence Medical College, Tokorozawa, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Takayama
authors.forename: Yuji
authors.initials: Y
authors.lastname: Koike
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohkawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Majima
authors.forename: Masashi
authors.initials: M
authors.lastname: Fukasawa
authors.forename: Nariyoshi
authors.initials: N
authors.lastname: Shinomiya
authors.forename: Takanori
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Mieno
authors.initials: M
authors.lastname: Konishi
authors.forename: Hoshio
authors.initials: H
authors.lastname: Hiraide
authors.forename: Takushi
authors.initials: T
authors.lastname: Tadakuma
authors.forename: Shuhji
authors.initials: S
authors.lastname: Seki
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000276:immunology; D001483:Base Sequence; D019002:CD56 Antigen / Q000097:blood; D018959:CD57 Antigens / Q000097:blood; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D053222:Fas Ligand Protein; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D008875:Middle Aged; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000097:blood* / Q000235:genetics; D015375:Receptors, Interleukin-2 / Q000097:blood; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D019002:CD56 Antigen; D018959:CD57 Antigens; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D015375:Receptors, Interleukin-2; D007371:Interferon-gamma