Deciphering metabolic networks.
All higher organisms divide major biochemical steps into different cellular compartments and often use tissue-specific division of metabolism for the same purpose. Such spatial resolution is accompanied with temporal changes of metabolite synthesis in response to environmental stimuli or developmental needs. Although analyses of primary and secondary gene products, i.e. transcripts, proteins, and metabolites, regularly do not cope with this spatial and temporal resolution, these gene products are often observed to be highly coregulated forming complex networks. Methods to study such networks are reviewed with respect to data acquisition, network statistics, and biochemical interpretation.
Fiehn
Oliver
O
Max-Planck Institute of Molecular Plant Physiology, 14424 Potsdam/Golm, Germany. fiehn@mpimp-golm.mpg.de
Weckwerth
Wolfram
W
D000818:Animals; D019295:Computational Biology; D005786:Gene Expression Regulation; D006801:Humans; D008660:Metabolism; D008954:Models, Biological; D008957:Models, Genetic; D040901:Proteomics; D015398:Signal Transduction