Adaptation of the phosphotungstate method for the determination of vitamin C contents in animal and human tissues.
The usefulness of phosphotungstate reagent for vitamin C determination in tissue homogenates has been confirmed. An optimal homogenization medium was selected: 1.8 M solution of HPO3 in 1.3 M CH3COOH. With this medium the analytical curve (at 700 nm) demonstrated the right linearity, correlation and recovery coefficients were appropriately high (0.999 and 99.8%) and the values of intraserial and interserial variation coefficient were low (< 5% and < 10%, respectively). It makes this method sensitive, easily repeatable, and useful for vitamin C determination in animal and human tissues, including neoplastic ones.
Rutkowski
Maciej
M
Department of Biochemistry and Chemistry, Institute of Basic Sciences, Military Medical University, Pl. J. Hallera 1, 90-647 Lódź, Poland. marek@achilles.wam.Lodz.pl
Grzegorczyk
Krzysztof
K
Greger
Janusz
J
D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D010772:Phosphotungstic Acid; D015203:Reproducibility of Results; D012996:Solutions
D007202:Indicators and Reagents; D012996:Solutions; D010772:Phosphotungstic Acid; D001205:Ascorbic Acid