title: Mtp-40 and alpha antigen gene fragment amplification for the detection of Mycobacterium tuberculosis in Colombian clinical specimens.
abstract: In this study, the use of Mtp-40 and alpha antigen polymerase chain reaction (PCR) amplification fragments for the precise tuberculosis (TB) diagnosis was evaluated. One hundred and ninety two different samples were obtained from 113 patients with suspected TB. Mtp-40 and alpha antigen protein genes were amplified by the PCR technique and compared to both the "gold standard" (culture) test, as well as the clinical parameters (including a clinical record and X-ray film exam in 113 patients). Thirty-eight of the 113 patients had a presumptive clinical diagnosis of TB; 74% being detected by PCR technique, 58% by culture and 44% by direct microscopic visualization. Weconclude that it is possible to use PCR as a suitable technique for the detection of any mycobacteria by means of the alpha antigen product, or the specific infection of Mycobacterium tuberculosis by means of the mtp-40 gene. This might be a good supporting tool in difficult clinical TB diagnosis and pauci-bacillary cases.
authors.affiliation: Departamento de Biología Molecular, Instituto de Inmunología de Colombia, Universidad Nacional de Colombia, Bogotá, Colombia. rosalba_alonso@fidic.org.co
authors.forename: Rosalba
authors.initials: R
authors.lastname: Alfonso
authors.forename: Rosa Elena
authors.initials: RE
authors.lastname: Romero
authors.forename: Manuel Elkin
authors.initials: ME
authors.lastname: Patarroyo
authors.forename: Luis Angel
authors.initials: LA
authors.lastname: Murillo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000032:analysis* / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D003105:Colombia; D005784:Gene Amplification; D006801:Humans; D007223:Infant; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
chemical_list: D000942:Antigens, Bacterial; C052242:alpha antigens, Mycobacterium