title: [RP-HPLC determination of diphenytriazol in rat liver microsomal incubates and its application in in vitro metabolism].
abstract: OBJECTIVE
  To establish a RP-HPLC method for determination of diphenytriazol (DL111-IT) in rat hepatic microsomes.
  
  
  METHODS
  DL111-IT in rat hepatic microsomal incubates was extracted with chloroform, using diazepam as internal standard. The determination was performed on a Lichrospher ODS-C18 reversed column (25 cm x 0.46 cm ID) with mobile phase of methanol-pH 7.5 phosphate buffer (70:30) at a flow-rate of 1.0 mL.min-1. A UVVIS detector was operated at 235 nm.
  
  
  RESULTS
  The assaywas linear from 1.01-101.0 micrograms.mL-1 for DL111-IT. The limit of detection was 0.15 microgram.mL-1 (signal-to-noise ratio 3) and the limit of quantification was 1.01 micrograms.mL-1(RSD < 10%, n = 4). The method afforded average recoveries of (100.3 +/- 1.9)% (n = 5), and intra-day and inter-day RSD were less than 5.0%(n = 5). The method allowed study of the in vitro phase I metabolism of DL111-IT in rat liver microsomal incubates. The microsomes induced by beta-naphthoflavone showed high enzymatic activity for DL111-IT phase I metabolism.
  
  
  CONCLUSIONS
  The method is simple, accurate and can be used to study the metabolism of DL111-IT in rat hepatic microsomes.
authors.affiliation: School of Pharmacy, Zhejiang University, Hangzhou 310031, China. Yaotw@cps.edu.cn
authors.forename: Tong-wei
authors.initials: TW
authors.lastname: Yao
authors.forename: Yun-zhen
authors.initials: YZ
authors.lastname: Hu
mesh_terms: D000020:Abortifacient Agents, Nonsteroidal / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D002469:Cell Separation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014230:Triazoles / Q000032:analysis* / Q000378:metabolism
chemical_list: D000020:Abortifacient Agents, Nonsteroidal; D014230:Triazoles; C030266:3-(2-ethylphenyl)-5-(3-methoxyphenyl)-1H-1,2,4-triazole