[Two restriction fluorescence labeling methods for enhancing the signal-to-noise ratio of cDNA microarray hybridization].
OBJECTIVE
To study the signal-to-noise ratio (SNR) of two restricted fluorescence labeling methods for examining gene expression profile by microarray hybridization.


METHODS
Samples of Saccharomyces cerevisiae mRNA was labeled by traditional reverse transcription method and 2 restriction fluorescent labeling methods using respectively Cy-universal primer and extension incorporated Cy-dNTP. The labeled samples were examined by the microarray, followed by washing and scanning under the same conditions.


RESULTS
The two restriction labeling methods showed superior results with lowered background and enhanced SNR and sensitivity, and Cy-universal primer labeling presented the best results.


CONCLUSIONS
SNR can be enhanced by the restriction labeling methods, which improve the applicability of microarray technology.
Shi
Rong
R
Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China. shirong@fimmu.edu.cn
Ma
Wen-li
WL
Song
Yan-bin
YB
Li
Ling
L
Liu
Cui-hua
CH
Zheng
Wen-ling
WL
D005453:Fluorescence; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012441:Saccharomyces cerevisiae