title: The preliminary study of interferon-gamma gene transfection to human Tenon's capsule fibroblasts in vitro.
abstract: OBJECTIVE
  To investigate the results of the interferon-gamma (IFN-gamma) gene transfer and transient expression in human Tenon's capsule fibroblast in vitro in order to find a way to gene therapy in vivo.
  
  
  METHODS
  Using LipofectAMINE, IFN-gamma gene was transferred in human Tenon's capsule fibroblasts with plasmid pcDNA3 IFN-gamma. Its mRNA transcription and protein expression were determined by RT-PCR and flow cytometry assay respectively.
  
  
  RESULTS
  The human Tenon's capsule fibroblasts transferred the IFN-gamma gene can express the IFN-gamma in transcription and protein level transiently.
  
  
  CONCLUSIONS
  IFN-gamma gene can be transferred successfully and expressed expressed efficiently in human tenon's capsule fibroblast in vitro.
authors.affiliation: Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Lan
authors.forename: J
authors.initials: J
authors.lastname: Ge
authors.forename: M
authors.initials: M
authors.lastname: Lin
authors.forename: J
authors.initials: J
authors.lastname: Zheng
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Wei
authors.forename: Y
authors.initials: Y
authors.lastname: Li
mesh_terms: D003228:Conjunctiva / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D018014:Gene Transfer Techniques; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D019076:Transgenes
chemical_list: D012333:RNA, Messenger; D007371:Interferon-gamma