Bifunctional effect of human IFN-gamma on cultured human fibroblasts from Tenon's capsule.
OBJECTIVE
To study the effect of human IFN-gamma on in vitro cultured human fibroblasts from Tenon's capsule.


METHODS
The effect of different concentrations of human IFN-gamma and mitomycin-C (MMC), 5-fluorouracil (5-Fu) on cultured human Tenon's capsule fibroblasts (HTCF) was measured using a MTT [3-(4, 5-dimethylthiazo-2-yl)]-2, 5-diphenyltetrazolium bromide; Thiazolyl blue) colorimetric assay. The results were analyzed using ANOVA of the statistical package for social sciences (SPSS) 9.0 version. The difference was considered to be significant if P < 0.05.


RESULTS
The effects of MMC and 5-Fu on the growth of HTCF were negative, while the effects of IFN-gamma on the growth of HTCF were both negative (10(2)-10(4) units/ml in two experiments) and positive (10(6), 10(5), 10 units/ml in two experiments). The inhibition rate of MMC ranged from 5.73% to 46.9%, which was similar to the inhibition rate of 5-Fu ranged from 12.49% to 38.92% (P = 0.351). The inhibition rate of IFN-gamma in two experiments was smaller than MMC and 5-Fu (P < 0.05).


CONCLUSIONS
IFN-gamma has bifunctional effect (both enhancement and inhibition) on proliferation of cultured HTCF. The antiproliferative effect of IFN-gamma was weaker than MMC and 5-Fu. Further study has to be carried out to document the inhibition of scar formation of filtration bleb by IFN-gamma and the molecular mechanisms of its bifunctional effect on HTCF proliferation.
Guo
Y
Y
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Ge
J
J
Liu
H
H
Li
Y
Y
Zheng
J
J
Huang
X
X
Lan
Y
Y
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003228:Conjunctiva / Q000166:cytology*; D003238:Connective Tissue / Q000187:drug effects*; D005123:Eye / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D016685:Mitomycin / Q000494:pharmacology; D011994:Recombinant Proteins
D011994:Recombinant Proteins; D016685:Mitomycin; D007371:Interferon-gamma; D005472:Fluorouracil