Mechanism of the protective effect of heteropolyoxotungstate against herpes simplex virus type 2.
The effects of heteropolyoxotungstate (K(7)[PTi(2)W(10)O(40)]. 6H(2)O; PM-19) on the replication of herpes simplex virus type 2 (HSV-2) were examined using a semiquantitative polymerase chain reaction of intracellular viral DNA established by us and also other methods. Vero cells were infected with HSV-2 strains: either the standard strain 169, or the acyclovir-resistant strain YS-4C-1. PM-19 was added at various stages during the replication of HSV-2. PM-19 strongly inhibited the synthesis of viral genomic DNA when it was added at the time of infection. The addition of PM-19 60-90 min after viral inoculation time-dependently decreased the antiviral activity and increased the relative yield of viral DNA, and the addition of PM-19 was completely ineffective at times later than 90 min. These results suggested that PM-19 inhibited viral penetration but did not affect the synthesis of viral DNA. Furthermore, PM-19 strongly inhibited a second round of infection.
Dan
Katsuaki
K
Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan. kdanmrw@sc.itc.keio.ac.jp
Miyashita
Kiichi
K
Seto
Yoshiko
Y
Fujita
Haruhisa
H
Yamase
Toshihiro
T
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000502:physiology; D018258:Herpesvirus 2, Human / Q000187:drug effects* / Q000502:physiology; D007424:Intracellular Fluid / Q000737:chemistry / Q000378:metabolism / Q000821:virology; D011108:Polymers / Q000494:pharmacology*; D017973:Tungsten Compounds / Q000494:pharmacology*; D014709:Vero Cells; D014771:Virion / Q000187:drug effects / Q000378:metabolism
D000998:Antiviral Agents; D004279:DNA, Viral; D011108:Polymers; D017973:Tungsten Compounds; C064098:polyoxotungstate PM 19