title: [Pharmacokinetics and bioavailability of clinafloxacin in rats].
abstract: OBJECTIVE
  To study the pharmacokinetics and bioavailability of clinafloxacin in rats.
  
  
  METHODS
  The drug concentration was determined by HPLC. The main pharmacokinetic parameters were obtained by 3P87 program. An RP-C18 was used as the stationary phase. The mobile phase was a mixture of acetonitrile-0.05 mol.L-1 citric acid triethylamine (pH 2.5) (20:80). The flow rate was 1.0 mL.min-1. The UV absorbance detector was set at 300 nm.
  
  
  RESULTS
  A good linearity was obtained from 0.03-20 micrograms.mL-1 of clinafloxacin in rat plasma with gamma = 0.9998. The plasma concentration--time curve of clinafloxacin conformed to one compartment open model. After ig administration of 50 mg.kg-1 and 100 mg.kg-1 dose of clinafloxacin in six rats, mean Cmax and AUC values increased in proportion to dose. Mean T1/2 appeared to be independent of dose. Mean AUC was 65 +/- 6 and 27 +/- 4 micrograms.h.mL-1 respectively after i.v. and ig administration of 100 mg.kg-1 dose. The extent of bioavailability (F) of clinafloxacin was 42%.
  
  
  CONCLUSIONS
  The results of the pharmacokinetic study of clinafloxacin showed that it exhibited first order kinetic characteristics and the bioavailability is low.
authors.affiliation: Department of Analytical Chemistry, School of Pharmacy, Shanghai Medical University, Shanghai 200032, China. mhzhou@eastday.com
authors.forename: M H
authors.initials: MH
authors.lastname: Zhou
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Yu
authors.forename: G L
authors.initials: GL
authors.lastname: Duan
authors.forename: W B
authors.initials: WB
authors.lastname: Cheng
authors.forename: C J
authors.initials: CJ
authors.lastname: Xu
authors.forename: X
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000493:pharmacokinetics*; D019540:Area Under Curve; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D024841:Fluoroquinolones; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; C057477:clinafloxacin