[Study on chemical constituents of rhizome of Anemone raddeana].
OBJECTIVE
To investigate the chemical constituents of the rhizome of Anemone raddeana Regel, so as to find new active compounds.


METHODS
The ethanol extracts of the rhizome of Anemone raddeana were obtained by silica column, HPLC. The structures of the compounds were elucidated by means of physico-chemical method and spectral analysis (IR, FAB-MS, 1HNMR, 13CNMR, DEPT, 1H-1H COSY, HMQC, HMBC).


RESULTS
Nine compounds were isolated and identified as 27-hydroxyolean-12(13)-en-28-oic acid-3-0-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranoside (1), eleutheroside K (2), Oleanolic acid-3-O-alpha-L-rhamnopyranosyl-(1-->2)-[beta-D- glucopyranosyl-(1-->4)]-alpha-L-arabinopyranoside (3), betulin (4), betulic acid (5), acetyloleamolic acid (6), evonymitol (7), oleamolic acid (8) and diosgenin (9).


CONCLUSIONS
Compound 1 is a new compound, named raddeanoside 12. Compounds 3-7 were isolated from this plant for the first time.
Lu
Jin-cai
JC
Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110016, China. jincailu99@yahoo.com
Xu
Bei-bei
BB
Zhang
Xin-yan
XY
Sun
Qi-shi
QS
D031883:Anemone / Q000737:chemistry*; D028001:Eleutherococcus; D015394:Molecular Structure; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification; D000094062:Betulinic Acid
C485708:27-hydroxyolean-12(13)-en-28-oic acid-3-O-rhamnopyranosyl-1-2-arabinopyranoside; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C029073:eleutherosides; C002503:betulin; D000094062:Betulinic Acid