Accurate mass measurements by Fourier transform mass spectrometry in the study of advanced glycation end products/peptides.
The Maillard reaction occurring between sugars and amino groups is important in living systems. When amino groups belonging to protein chains are involved, the Maillard reaction has been invoked as responsible for protein cross-linking and the production of 'toxic' compounds. The reaction leads to the production of a heterogeneous group of substances, usually called advanced glycation end products (AGEs). Classical analytical approaches, such as spectroscopic (ultraviolet, fluorescence) and mass spectrometric (matrix-assisted laser desorption/ionization, liquid chromatography/electrospray ionization mass spectrometry) methods, have shown that the digestion mixture is highly complex. However, there are clear differences between the digestion mixtures of glycated and unglycated human serum albumin (HSA). In the former case, possible glycated peptides belonging to the AGE peptide class may be identified. Tandem mass spectrometric experiments on selected species seemed to be promising as regards structural information, but it was thought of interest to undertake the present investigation, based on liquid chromatography/electrospray ionization Fourier transform mass spectrometry, in order to obtain definitive results on their elemental composition. Using this approach, about 20 glycated peptides were detected and their possible structures were postulated by examining the known sequence of HSA.
Marotta
Ester
E
CNR, Istituto di Scienze e Tecnologie Molecolari, Corso Stati Uniti 4, 35100 Padova, Italy.
Lapolla
Annunziata
A
Fedele
Domenico
D
Senesi
Antonella
A
Reitano
Rachele
R
Witt
Matthias
M
Seraglia
Roberta
R
Traldi
Pietro
P
D000595:Amino Acid Sequence; D002853:Chromatography, Liquid / Q000379:methods; D005947:Glucose / Q000737:chemistry; D017127:Glycation End Products, Advanced / Q000032:analysis*; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000032:analysis*; D010449:Peptide Mapping / Q000379:methods*; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D014357:Trypsin / Q000378:metabolism; D000093423:Glycated Serum Albumin
D017127:Glycation End Products, Advanced; D009842:Oligopeptides; D012709:Serum Albumin; D014357:Trypsin; D005947:Glucose; D000093423:Glycated Serum Albumin