[Localization and characterization of partial immunodominant antigen epitopes of Trichinella spiralis].
OBJECTIVE
To screen and characterize immunodominant antigen epitopes on the soluble antigens of Trichinella spiralis (T.s.).


METHODS
15 monoclonal antibodies (McAbs) against T.s. muscle larva(ML) soluble antigens were obtained by using hybridoma technique. The reactivity of monoclonal and polyclonal antibodies were tested by ELISA, Western blotting and indirect immunofluorescence assay(IFA).


RESULTS
The Western blotting result showed that of the 15 McAbs, 6 could bind to the T.s. ML antigens displaying molecular weights of 40-70 kDa. Polyclonal sera could react with more than 10 bands having molecular weights of 20-200 kDa. Among the 6 McAbs, 4 could recognize epitopes on the cuticle surface and the other two could recognize epitopes on both the cuticle surface and the stichosome.


CONCLUSIONS
The antigen epitopes of T.s. recognized by 6 McAbs had been characterized.
Zhan
Y A
YA
Department of Biology, East China Normal University, Shanghai 200062.
Yan
Z Z
ZZ
Wan
W
W
Lu
Z Y
ZY
Zhu
Z Q
ZQ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D000947:Antigens, Helminth / Q000032:analysis*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D016056:Immunodominant Epitopes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014234:Trichinella / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D016056:Immunodominant Epitopes