title: Genetic variability of the frameshift region in IS911 transposable elements from Escherichia coli clinical isolates.
abstract: The IS911 bacterial transposable element has been analyzed for its mechanism of transposition and for the way it controls the expression of its genes by programmed -1 translational frameshifting. In the present study the prevalence of IS911 has been determined in the Enterobacteriaceae family and in other Gram-negative bacilli. Three variants, found in Escherichia coli clinical isolates and having mutations in the region implicated in frameshifting, were functionally characterized. All three were altered in their frameshifting and transposition abilities, suggesting that the frameshift region of IS911 may constitute a target for mutations reducing the transposition frequency of this mobile element in natural populations of E. coli.
authors.affiliation: Laboratoire de Microbiologie et Génétique Moléculaires, CNRS UMR5100, 118 route de Narbonne, 31062 Cedex, Toulouse, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Licznar
authors.forename: Claire
authors.initials: C
authors.lastname: Bertrand
authors.forename: Isabelle
authors.initials: I
authors.lastname: Canal
authors.forename: Marie Françoise
authors.initials: MF
authors.lastname: Prère
authors.forename: Olivier
authors.initials: O
authors.lastname: Fayet
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D016368:Frameshift Mutation; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008957:Models, Genetic; D016254:Mutagenesis, Insertional
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial