Gelatinolytic activity of matrix metalloproteinase in lung cancer studied using film in situ zymography stamp method.
In this study, we investigated activity of matrix metalloproteinase (MMP) of lung cancer by newly developed film in situ zymography (FIZ) stamp method, which allows visual localization of gelatinolytic activity within the cut surface of a tumor. We performed FIZ stamp method and conventional gelatin zymography in 39 resected specimen of lung cancer. The degree of gelatinolytic activity was scored (FIZ score) and correlated with the clinicopathological factors of the tumor. FIZ score of normal lung was very low. Lung cancer tissue had consistently higher FIZ score than the matched normal lung tissue. There were statistically significant differences in the FIZ score according to the pathological stage (P = 0.0015), nodal status (P = 0.0007) and lymphatic invasion (P = 0.0004). Direct correlation was observed between the FIZ score and MMP-2 activity (rho = 0.568, P = 0.0030) as quantitated using conventional gelatin zymography. MMP-2 may play an important role in the lymphatic invasion of lung cancer. FIZ stamp method may be a simple and useful diagnostic aid for the presence of cancer cells in the resected specimen.
Kaji
Masahiro
M
Department of Surgery II, Nagoya City University Medical School, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.
Moriyama
Satoru
S
Sasaki
Hidefumi
H
Saitoh
Yushi
Y
Kiriyama
Masanobu
M
Fukai
Ichiro
I
Yamakawa
Yosuke
Y
Mitsui
Akira
A
Toyama
Tatsuya
T
Nemori
Ryoichi
R
Fujii
Yoshitaka
Y
D000230:Adenocarcinoma / Q000201:enzymology / Q000473:pathology; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D018287:Carcinoma, Large Cell / Q000201:enzymology / Q000473:pathology; D018288:Carcinoma, Small Cell / Q000201:enzymology / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D005780:Gelatin / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000473:pathology; D009367:Neoplasm Staging
D014408:Biomarkers, Tumor; C015233:gelatin film; D005780:Gelatin; D020778:Matrix Metalloproteinase 2