title: [Immunostimulatory activity elicited by CpG sequences in Cysticercus cellulosae paramyosin cDNA].
abstract: OBJECTIVE
  To analyse the immunostimulatory activity of CpG sequences in cysticercus cellulosae paramyosin (also named Antigen B, AgB) cDNA.
  
  
  METHODS
  C57BL/6 mice were immunized with pcDNA3-AgB plasmid, pcDNA3-AgB' (CpG sequences were mutated), pcDNA3 or AgB protein and two weeks later, immune response was assayed by ELISA.
  
  
  RESULTS
  IgG and IgG2a were detectable at week 2 after immunization and continually increased until week 4. The antibody levels elicited by pcDNA3-AgB were significantly higher(P < 0.05) than those elicited by others.
  
  
  CONCLUSIONS
  After pcDNA3-AgB plasmid inoculation, the immune response of mouse was elicited not only by the AgB protein but also by the CpG immunostimulatory sequences in the AgB cDNA.
authors.affiliation: Department of Medical Genetic, Second Military Medical University, Shanghai 200433.
authors.forename: S H
authors.initials: SH
authors.lastname: Sun
authors.forename: Y J
authors.initials: YJ
authors.lastname: Guo
authors.forename: Q M
authors.initials: QM
authors.lastname: Wang
authors.forename: R W
authors.initials: RW
authors.lastname: Chen
mesh_terms: D000818:Animals; D018899:CpG Islands / Q000276:immunology*; D003552:Cysticercus / Q000276:immunology*; D018076:DNA, Complementary / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids / Q000276:immunology; D014335:Tropomyosin / Q000235:genetics*; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D018076:DNA, Complementary; D007074:Immunoglobulin G; D014335:Tropomyosin; D019444:Vaccines, DNA