title: Analysis of gene expression in human colorectal cancer tissues by cDNA array.
abstract: BACKGROUND
  The development and progression of cancer are accompanied by complex changes in patterns of gene expression. The purpose of this study was to clarify the relevance of macroarray analysis of human colorectal cancer tissues.
  
  
  METHODS
  Hybridization of cDNA macroarray filters on which 550 genes had been spotted was performed with biotin-labeled cDNA targets that were prepared from mRNA extracted from 20 pairs of colorectal cancer and corresponding noncancerous tissues. Expression of differentially expressed genes was further studied by semiquantitative RT-PCR.
  
  
  RESULTS
  Fourteen (2.5%) of the 550 genes were differentially expressed and up- or downregulated in cancer tissues by at least threefold compared with matched noncancerous tissues in 10 or more of the 20 patients. The genes that were upregulated in cancer tissues were associated with transcription, cell cycle, growth factor receptor, cell adhesion, extracellular matrix-degrading enzymes, and angiogenesis, and the downregulated genes were those involved in apoptosis and immune recognition. Semiquantitative RT-PCR analysis of these differentially expressed genes gave results consistent with those by cDNA array analysis.
  
  
  CONCLUSIONS
  Although the macroarray used in this study contained only a small number of genes, our results support the feasibility and usefulness of this approach to study variation in gene expression patterns in human colorectal cancer tissues. The results also suggest the possibility of a diagnostic application of cDNA macroarrays in daily clinical settings.
authors.affiliation: First Department of Internal Medicine, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, Sapporo 060-8543, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Arisa
authors.initials: A
authors.lastname: Imsumran
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fukushima
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Adachi
authors.forename: Yongfen
authors.initials: Y
authors.lastname: Min
authors.forename: Shouhei
authors.initials: S
authors.lastname: Iku
authors.forename: Shina
authors.initials: S
authors.lastname: Horiuchi
authors.forename: Mio
authors.initials: M
authors.lastname: Yoshida
authors.forename: Kazuko
authors.initials: K
authors.lastname: Shimada
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sasaki
authors.forename: Fumio
authors.initials: F
authors.lastname: Itoh
authors.forename: Takao
authors.initials: T
authors.lastname: Endo
authors.forename: Kohzoh
authors.initials: K
authors.lastname: Imai
mesh_terms: D001707:Biopsy, Needle; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D046508:Culture Techniques; D018076:DNA, Complementary / Q000032:analysis*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012494:Sampling Studies; D012680:Sensitivity and Specificity
chemical_list: D018076:DNA, Complementary