[Antibacterial activity of Chinese medicine kou-kang mouthwash and its acute toxicity test].
OBJECTIVE
To study the antibacterial activity of Chinese medicine kou-Kang mouthwash and its acute toxicity, and provide the basis of experiment and therapy for infectious diseases of the oral cavity.


METHODS
Minimal inhibitory concentrations (MIC) of kou-Kang mouthwash were determined by the agar dilution method, and the acute toxicity was done.


RESULTS
The range of MIC kou-Kang mouthwash against 27 Gram negative bacterial were 0.03-1.0 g.ml-1. MIC50 was 0.125 g.ml-1 and MIC90 was 0.125-0.5 g.ml-1. The range of MIC kou-Kang mouthwash against 21 Gram positive bacterial was 0.008-0.5 g.ml-1. The range of MIC50 and MIC90 were 0.015-0.125 g.ml-1 and 0.015-0.5 g.ml-1, respectively. The value of MIC was 0.015 g.ml-1 against candidia albicans. The acute toxic test indicated that the maximum tolerance dose was 417.6 g.kg-1 and 210 times as much as the clinical dose a day. No death of animals or toxicity and side-effect occurred in the test.


CONCLUSIONS
Kou-Kang mouthwash shows a potent antibacterial activity against various pathogens of the oral cavity. Its antibiotic activity against Gram positive bacteria was 2-8 times stronger than that against Gram negative bacteria. It has low toxicity and can be used in clinical application.
Qin
Qun
Q
Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, China.
Xie
Zhao-xia
ZX
Pi
Hai-zhen
HZ
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009067:Mouthwashes / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects*; D013280:Stomatitis / Q000382:microbiology
D000900:Anti-Bacterial Agents; D004365:Drugs, Chinese Herbal; D009067:Mouthwashes