[Comparative study of modulating effect of kidney tonifying recipe and spleen invigorating recipe on T-lymphocyte apoptosis in corticosterone treated rats].
OBJECTIVE
To compare the modulating effect of Kidney tonifying recipe (KTR) and Spleen invigorating recipe (SIR) on T-lymphocyte apoptosis (TLA) in corticosterone treated rats (CTR).


METHODS
Qualitative and quantitative analysis on activation-induced TLA was conducted by transmission electron microscope and TUNEL labelled flow cytometry detection.


RESULTS
TLA percentage of CTR was 45.87 +/- 7.22%, while that of normal control rats was 34.25 +/- 6.47%, the difference between them was significant (P < 0.01). TLA percentage of Youguiyin treated rats was 35.90 +/- 7.39%, and that of Bushen Yishou capsule treated rats was 36.20 +/- 9.14%, compared with that of CTR, P < 0.01 and P < 0.05 respectively. TLA percentage of the SIR treated group was 36.92 +/- 11.82%, which was different insignificantly as compared with that of CTR.


CONCLUSIONS
TLA susceptibility was significantly enhanced in CTR. Both KTRs (Youguiyin and Bushen Yishou capsule) had down-regulating effect on TLA, while the effect of SIR was insignificant, suggesting that down-regulating activation-induced TLA may be one of the important mechanisms of KTR in improving T-lymphocyte function in CTR.
Chen
Yu
Y
Institute of Integrated Traditional Chinese and Western Medicine, Huashan Hospital, Fudan University, Shanghai 200040.
Shen
Zi-yin
ZY
Chen
Wei-hua
WH
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D003345:Corticosterone; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013601:T-Lymphocytes / Q000166:cytology* / Q000648:ultrastructure; D016711:Yang Deficiency / Q000139:chemically induced / Q000276:immunology*
D004365:Drugs, Chinese Herbal; D003345:Corticosterone