Surfactant strengthens the inhibitory effect of C-reactive protein on human lung macrophage cytokine release.
In this study we investigated the effect of acute-phase levels of C-reactive protein (CRP) on cytokine production by pulmonary macrophages in the presence or absence of pulmonary surfactant. Both human alveolar and interstitial macrophages as well as human surfactant were obtained from multiple organ donor lungs. Precultured macrophages were stimulated with LPS alone or together with IFN-gamma in the presence or absence of CRP, surfactant, and combinations. Releases of TNF-alpha and of IL-1beta to the medium were determined. We found that CRP could modulate lung inflammation in humans by decreasing the production of proinflammatory cytokines by both alveolar and interstitial macrophages stimulated with LPS alone or together with IFN-gamma. The potential interaction between CRP and surfactant phospholipids did not overcome the effect of either CRP or surfactant on TNF-alpha and IL-1beta release by lung macrophages. On the contrary, CRP and pulmonary surfactant together had a greater inhibitory effect than either alone on the release of proinflammatory cytokines by lung macrophages.
Casals
Cristina
C
Department of Biochemistry, San Carlos Hospital, Complutense University of Madrid, 28040 Madrid, Spain. ccasalsc@bio.ucm.es
Arias-Diaz
Javier
J
Valino
Fernando
F
Saenz
Alejandra
A
Garcia
Cruz
C
Balibrea
Jose L
JL
Vara
Elena
E
D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002097:C-Reactive Protein / Q000494:pharmacology*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D004357:Drug Synergism; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007375:Interleukin-1 / Q000096:biosynthesis; D008168:Lung / Q000737:chemistry / Q000166:cytology; D016676:Macrophages, Alveolar / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011663:Pulmonary Surfactants / Q000302:isolation & purification / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D016207:Cytokines; D007375:Interleukin-1; D011663:Pulmonary Surfactants; D014409:Tumor Necrosis Factor-alpha; D002097:C-Reactive Protein