title: A new murine monoclonal antibody against Kx protein.
abstract: Mice immunized with a synthetic peptide located on an intracellular segment of the polytopic Kx protein (37 kDa) from human red blood cells (RBCs) produced a monoclonal antibody called C7B8. As expected, this antibody did not agglutinate common RBCs but reacted with permeabilized cells in flow cytometry. C7B8 recognizes the Kx protein on Western blots. Cross-reactivity of C7B8 with human calpain of human muscle extracts was demonstrated by Western blot analysis. This cross-reactivity precludes the use of C7B8 for Kx tissue distribution studies, but immobilized C7B8 was a convenient tool for purification of the Kell-Kx complex from RBC membrane extract by immunochromatography.
authors.affiliation: INSERM U76, Institut National de la Transfusion Sanguine, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Hattab
authors.forename: D
authors.initials: D
authors.lastname: Blanchard
authors.forename: P
authors.initials: P
authors.lastname: Gane
authors.forename: V
authors.initials: V
authors.lastname: Verkarre
authors.forename: Y
authors.initials: Y
authors.lastname: Petit-Leroux
authors.forename: M-J
authors.initials: MJ
authors.lastname: Loirat
authors.forename: J-P
authors.initials: JP
authors.lastname: Cartron
authors.forename: O
authors.initials: O
authors.lastname: Bertrand
mesh_terms: D026921:Amino Acid Transport Systems, Neutral / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D000937:Antigen-Antibody Reactions; D015153:Blotting, Western; D002154:Calpain / Q000276:immunology; D002846:Chromatography, Affinity; D003429:Cross Reactions; D005434:Flow Cytometry; D006801:Humans; D007626:Kell Blood-Group System
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D000911:Antibodies, Monoclonal; D007626:Kell Blood-Group System; C087883:XK protein, human; D002154:Calpain