Oxidative cleavage of DNA by tridentate copper (II) complex.
Copper (II) complex 1 having planar tridentate ligand, bzimpy, where bzimpy is 2,6-bis(benzimidazo-2-yl) pyridine was synthesized and characterized by UV-visible, FAB (fast atom bombardment) mass and infrared spectroscopy. From absorption titration data, the binding constant of Cu(II) with DNA was calculated to be (1.8+/-0.02)x10(4) M(-1). Thermal denaturation study of DNA with 1 revealed deltaT(m) of 5+/-0.5 degrees C. Viscosity measurement showed that complex binds with DNA through intercalative mode. Copper (II) complex induces cleavage in plasmid DNA in the presence of coreductants such as ascorbic acid or glutathione.
Vaidyanathan
Vaidyanathan Ganesan
VG
Chemical Laboratory, Central Leather Research Institute, Adyar, 600 020 Chennai, India.
Nair
Balachandran Unni
BU
D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D007364:Intercalating Agents / Q000737:chemistry / Q000494:pharmacology; D008024:Ligands; D013058:Mass Spectrometry; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D009942:Organometallic Compounds / Q000737:chemistry* / Q000494:pharmacology; D010084:Oxidation-Reduction; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D013057:Spectrum Analysis; D013696:Temperature
D007364:Intercalating Agents; D008024:Ligands; D009942:Organometallic Compounds; D003300:Copper; D004247:DNA