[The diagnostic value of combined detection of circulating antigens and antibodies in urine of patients with schistosomiasis japonica].
OBJECTIVE
To assess the value of detecting circulating antigens and antibodies in urine as a noninvasive method for the diagnosis of schistosomiasis.


METHODS
A sandwich ELISA and ELISA using McAb were applied to detect circulating schistosomal antigens and specific antibodies in the urine of patients with acute and chronic schistosomiasis.


RESULTS
When the urine samples from 10 cases of acute schistosomiasis and 61 cases of chronic schistosomiasis were examined, the positive rates of circulating antigens and specific antibodies were 60%, 40%, and 80%, 60.1%, respectively; when both detection was combined, the positive rates were 100% and 71.7% respectively, whereas a false positive rate of 3% of CAg or CAb was detected in the urine of 100 normal controls.


CONCLUSIONS
The detection of circulating antigen in urine is a practicable and noninvasive method for the diagnosis of schistosomiasis.
Zhang
E Y
EY
Department of Parasitology, Shanghai Second Medical University, Shanghai 200025.
Lou
W X
WX
Xue
C L
CL
Wang
Z J
ZJ
D000818:Animals; D000909:Antibodies, Helminth / Q000652:urine*; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis*
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth