A forkhead transcription factor FKHR up-regulates lipoprotein lipase expression in skeletal muscle.
Lipoprotein lipase (LPL) plays a role in lipid usage in skeletal muscle by hydrolyzing plasma triglycerides into fatty acids, which are further utilized for beta-oxidation. Lipid usage is stimulated during fasting, diabetes mellitus and exercise, concomitant with enhanced LPL expression in skeletal muscle. Here we show that the forkhead type transcription factor FKHR is strongly induced in skeletal muscle in fasting mice, in mice with streptozotocin-induced diabetes and in mice after treadmill running. Ectopic expression of FKHR enhanced LPL gene expression in C2C12 muscle cells in culture. These results implicate FKHR as an important modulator of lipid metabolism in skeletal muscle.
Kamei
Yasutomi
Y
PRESTO, Japan Science and Technology Corporation, National Institute of Health and Nutrition, 1-23-1, Toyama Shinjuku-ku, Tokyo 162-863, Japan. ykamei@nih.go.jp
Mizukami
Junko
J
Miura
Shinji
S
Suzuki
Miki
M
Takahashi
Nobuyuki
N
Kawada
Teruo
T
Taniguchi
Tomoyasu
T
Ezaki
Osamu
O
D000818:Animals; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000502:physiology*; D004790:Enzyme Induction; D005215:Fasting; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; D008071:Lipoprotein Lipase / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D032342:Muscle Cells / Q000378:metabolism; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000378:metabolism; D010805:Physical Conditioning, Animal; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic; D015854:Up-Regulation
D004268:DNA-Binding Proteins; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; C467200:Foxo1 protein, mouse; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D008071:Lipoprotein Lipase