[Immune response in mice induced by C terminal encoding gene of Plasmodium falciparum histidine rich protein. 2].
OBJECTIVE
To explore the humoral and cellular immune responses in mice to eukaryotic expression recombinant plasmid encoding histidine rich protein 2 (HRP-II) of Plasmodium falciparum.


METHODS
The start and stop codes were introduced into HRP-II gene fragment, the reading frame and the position of start and stop codes in HRP-II were identified by sequencing. HRP-II fragment containing the start and stop codes was cloned into pcDNA3.1 (-) to form pcDNA3.1 (-)/HRP-II. The BALB/c mice were immunized i.m. with the plasmids for 3 times in 3 weeks intervals. Two weeks after the last immunization, the sera and splenocytes were collected to investigate anti-HRP-II antibodies by ELISA and the splenocytes proliferation response to HRP-II.


RESULTS
Sequence data show that the reading frame and the position of start and stop codes are correct. Restriction enzyme digestion indicated that the HRP-II gene fragment containing start and stop codes was successfully cloned into pcDNA3.1 (-). Mice raised significant anti-HRP-II antibodies after pcDNA3.1 (-)/HRP-II immunization, and the splenocytes proliferated prominently when stimulated with HRP-II protein.


CONCLUSIONS
Eukaryotic expression recombinant plasmid encoding HRP-II gene can induce significantly humoral and cellular immune response in mice. HRP-II gene may be a good candidate for P. falciparum blood-stage multiple DNA vaccine.
Miao
J
J
Department of Etiology, Fourth Military Medical University, Xi'an 710032.
Li
X
X
Xue
C F
CF
Liu
Z X
ZX
Wang
X F
XF
Zhen
R F
RF
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D004274:DNA, Recombinant / Q000276:immunology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D010963:Plasmodium falciparum / Q000276:immunology*; D011506:Proteins / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*; D000099053:Histidine-Rich Glycoprotein
D000913:Antibodies, Protozoan; D004274:DNA, Recombinant; D011506:Proteins; D019444:Vaccines, DNA; D000099053:Histidine-Rich Glycoprotein