[Experimental study of compound salvia injection in preventing and treating chronic nephrotoxicity induced by cyclosporin A in rats].
OBJECTIVE
To explore the mechanism of protective effect of Compound Salvia Injection (CSI) on experimental cyclosporin A induced nephrotoxicity.


METHODS
Rats were on low-salt diet and cyclosporin A (CsA) was administered once a day through gastrogavage at dosage of 30 mg/kg.d for 28 days. Expression of the mRNA for intrarenal transforming growth factor-beta 1 (TGF-beta 1) and renin was measured by reverse transcription-polymerase chain reaction (RT-PCR). Intrarenal expression of TGF-beta 1 and Collagen IV was determined by immunohistochemical assays. The effects of CSI on these changes were also evaluated.


RESULTS
Chronic CsA-induced nephropathy might be correlated to TGF-beta 1 and renin mRNA up-regulation as well as matric proteins accumulation in interstitium. CSI could reduce these changes.


CONCLUSIONS
Decreased CsA-related TGF-beta 1 and renin upregulation expression and accumulation of matrix proteins in the kidney might be related to the protective mechanism of CSI on CsA-induced chronic nephrotoxicity.
Qiao
B P
BP
First Affiliated Hospital of Henan Medical University, Zhengzhou 450052.
Tang
X D
XD
Ruan
Q
Q
D000818:Animals; D002908:Chronic Disease; D024141:Collagen Type IV / Q000096:biosynthesis* / Q000235:genetics; D016572:Cyclosporine; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D009401:Nephrosis / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D008517:Phytotherapy; D010936:Plant Extracts; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012083:Renin / Q000096:biosynthesis / Q000235:genetics; D027561:Salvia miltiorrhiza; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics
D024141:Collagen Type IV; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D016572:Cyclosporine; D012083:Renin