Canine Bcl-xL gene and its expression in tumor cell lines.
The canine Bcl-xL gene was cloned and sequenced. Canine Bcl-xL cDNA clone was 1252 bp in length, and encoded 233 deduced amino acides. The predicted canine Bcl-xL amino acid sequence shared 99.6%, 97.0%, 97.9%, 98.7% and 98.3% homology with that of human, mouse, rat, sheep and pig Bcl-xL, respectively. RT-PCR analysis revealed that canine Bcl-xL mRNA was constitutively expressed in CL-1 (canine lymphoma) and GL-1 (canine B cell leukemia) cell lines.
Sano
Junichi
J
Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Japan.
Oguma
Keisuke
K
Kano
Rui
R
Hasegawa
Atsuhiko
A
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004283:Dog Diseases / Q000235:genetics; D004285:Dogs / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D007938:Leukemia / Q000235:genetics / Q000662:veterinary; D008223:Lymphoma / Q000235:genetics / Q000662:veterinary; D008969:Molecular Sequence Data; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured; D051020:bcl-X Protein
D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein