title: Helicobacter pylori strains and histologically-related lesions affect the outcome of triple eradication therapy: a study from southern Italy.
abstract: BACKGROUND
  Certain evidence suggests that Helicobacter pylori strains expressing genes for cytotoxin production show a higher sensitivity than non-cytotoxic organisms to eradication treatment. No data are available on the involvement of bacterium-related lesions in different therapeutic outcomes.
  
  
  OBJECTIVE
  (i) To investigate whether differences in eradication rates may be related to the different expression of virulent strains (cagA, vacA, iceA) in patients undergoing proton pump inhibitor-based triple therapy, and (ii) to evaluate whether therapeutic outcome may be affected by bacterium-induced gastric lesions.
  
  
  METHODS
  One hundred and ten H. pylori-positive subjects were enrolled. H. pylori was genotyped by polymerase chain reaction. Treatment consisted of lansoprazole-amoxicillin-clarithromycin, twice daily for 1 week. Eradication was checked by urea breath test.
  
  
  RESULTS
  The eradication rate was 70%, and the absence of cagA was associated with unsuccessful treatment. No difference between the groups with successful and unsuccessful eradication was found with regard to vacA and iceA. Lympho-epithelial lesions and fibrosis were associated with unsuccessful treatment.
  
  
  CONCLUSIONS
  The present data confirm the importance of cagA (but not vacA and iceA) as a predictor of successful eradication. When fibrosis and lympho-epithelial lesions are present, therapy appears to be less effective. Therefore, these histological features may be involved in an unsuccessful therapeutic outcome.
authors.affiliation: Laboratory of Biochemistry, Scientific Institute for Digestive Diseases IRCCS Saverio de Bellis, Castellana Grotte, Bari, Italy. irccsbiochimica@libero.it
authors.forename: F
authors.initials: F
authors.lastname: Russo
authors.forename: P
authors.initials: P
authors.lastname: Berloco
authors.forename: R
authors.initials: R
authors.lastname: Cuomo
authors.forename: M L
authors.initials: ML
authors.lastname: Caruso
authors.forename: G
authors.initials: G
authors.lastname: Di Matteo
authors.forename: P
authors.initials: P
authors.lastname: Giorgio
authors.forename: V
authors.initials: V
authors.lastname: De Francesco
authors.forename: A
authors.initials: A
authors.lastname: Di Leo
authors.forename: E
authors.initials: E
authors.lastname: Ierardi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005773:Gastroscopy; D005838:Genotype; D016481:Helicobacter Infections / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000145:classification* / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D016896:Treatment Outcome
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; C090404:UreE protein, Bacteria; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori; C063113:ice nucleation protein