[Effect of amniotic membrane on expressions of TGF-beta 1, collagens I, III and fibronectin in rabbit corneal healing after photorefractive keratectomy].
OBJECTIVE
To observed the effect of amniotic membrane transplantation (AMT) on expressions of transforming growth factor-beta 1(TGF-beta 1), collagens I, III and fibronectin (FN) in rabbit corneal healing after photorefractive keratectomy (PRK), and investigate the anti-scarring formation mechanism of AMT.


METHODS
Ten rabbits underwent bilateral PRK to correct 8 diopters of myopia. One eye was randomly transplanted with preserved human amniotic membrane, and the other eye served as the control. The expressions of TGF-beta 1, collagens I, III and FN were studied by immunohistochemistry at 4 weeks after operation.


RESULTS
The expression of TGF-beta 1 in corneal epithelium and keratocytes and collegans in anterior stroma was significantly less in AMT group than in the control group. The expressions of collagens III and FN in anterior stroma of ablation area were also significantly less in AMT group than in the control group. The staining of collagen I had no significant difference between the AMT group and the control group.


CONCLUSIONS
The expressions of TGF-beta 1, collagens III and FN can be suppressed by amniotic membrane after PRK. These data suggest that part of the anti-scarring effect of AMT may be mediated through the suppression of TGF-beta 1, collagens III and FN expression.
Zhong
Y
Y
Ophthalmic Center, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Zhai
Z
Z
Zhou
Y
Y
Ye
W
W
Wang
K
K
D000650:Amnion / Q000637:transplantation*; D000818:Animals; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D024061:Collagen Type III / Q000096:biosynthesis / Q000235:genetics; D003315:Cornea / Q000378:metabolism; D005260:Female; D005353:Fibronectins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D054018:Lasers, Excimer; D008297:Male; D009216:Myopia / Q000378:metabolism / Q000601:surgery; D018903:Photorefractive Keratectomy; D011817:Rabbits; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D053773:Transforming Growth Factor beta1; D014183:Transplantation, Heterologous; D014945:Wound Healing
D024042:Collagen Type I; D024061:Collagen Type III; D005353:Fibronectins; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1