title: Human spermatozoa motility analysis in a Ringer's solution containing cupric ions.
abstract: The ability of cupric ions to immobilize human spermatozoa was assessed in vitro using an optical microscope. A Ringer's solution containing human spermatozoa and three cupric ion concentrations of 8 x 10(-8) mol/L, 8 x 10(-6) mol/L and 8 x 10(-5) mol/L were tested. The higher cupric ion concentrations (8 x 10(-5) mol/L and 8 x 10(-6) mol/L) significantly reduced spermatozoa motility after about 20 min. However, with the lower cupric ion concentration tested (8 x 10(-8) mol/L), also for 20-min experimentation, the reduction in motility was more limited.
authors.affiliation: Biochemistry Section, Institute of Chemistry, Catholic University of Valparaíso, Avda Brasil 2950, Casilla 4059, Valparaíso, Chile.
authors.forename: R
authors.initials: R
authors.lastname: Araya
authors.forename: H
authors.initials: H
authors.lastname: Gómez-Mora
authors.forename: R
authors.initials: R
authors.lastname: Vera
authors.forename: J M
authors.initials: JM
authors.lastname: Bastidas
mesh_terms: D003300:Copper / Q000008:administration & dosage* / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007552:Isotonic Solutions / Q000008:administration & dosage* / Q000737:chemistry; D007700:Kinetics; D008297:Male; D000077331:Ringer's Solution; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects*
chemical_list: D007552:Isotonic Solutions; D003300:Copper; D000077331:Ringer's Solution