title: Prokaryote taxonomy of the 20th century and the impact of studies on the genus Pseudomonas: a personal view.
abstract: The taxonomic studies on the genus Pseudomonas performed in the Department of Bacteriology of the University of California at Berkeley played a significant rôle in the development of modern prokaryote taxonomy, which started in the 1960s. This impact was due to a revival of the method of den Dooren de Jong for the nutritional analysis of chemoorganotrophic organisms and, mostly, to the introduction of the determination of rRNA as a method of taxonomic analysis. While the introduction of the nutritional studies facilitated the characterization of Pseudomonas and other chemoorganotrophs, the applicability of the rRNA studies extended to all prokaryotes.
authors.affiliation: Department of Biochemistry and Microbiology, Rutgers University Cook College, New Brunswick, NJ 08901, USA. palleroni@aesop.rutgers.edu
authors.forename: Norberto J
authors.initials: NJ
authors.lastname: Palleroni
mesh_terms: D001419:Bacteria / Q000737:chemistry / Q000145:classification*; D015373:Bacterial Typing Techniques / Q000266:history; D001432:Bacteriology / Q000266:history; D002965:Classification / Q000379:methods*; D004275:DNA, Ribosomal / Q000032:analysis; D049672:History, 19th Century; D049673:History, 20th Century; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012335:RNA, Ribosomal