title: [Study on the technique of Rehmannia glutinosa virus-free].
abstract: The stem tip from germ-free stem segment of Rehmannia glutinosa cultured in test tube can be induced into virus-free seedling. The experiment showed that the proper disinfectant for stem segments of Rehmannia glutinosa was 0.05% HgCl2. The seedings from stem segments grew better with MS in the concentration of agar 0.7% and pH7. The stem tips could be directly induced to seedlings by using MS + 6-BA(0.05 mg/L). The MS media for seedlings virus-free culture are 1/4 macro-elements + 1/2 micro-elements and using edible sugar instead of sucrose, so the cost of media could be decreased to 48.7%.
authors.affiliation: Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng, Shanxi 044000.
authors.forename: Zongxin
authors.initials: Z
authors.lastname: Wu
authors.forename: Honge
authors.initials: H
authors.lastname: Xie
authors.forename: Wenlong
authors.initials: W
authors.lastname: Feng
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Hongxia
authors.initials: H
authors.lastname: Li
authors.forename: Jianghui
authors.initials: J
authors.lastname: Li
mesh_terms: D046508:Culture Techniques; D004202:Disinfectants / Q000494:pharmacology; D008627:Mercuric Chloride / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D010942:Plant Viruses / Q000187:drug effects; D010946:Plants, Medicinal / Q000254:growth & development* / Q000821:virology; D032267:Rehmannia / Q000254:growth & development* / Q000821:virology; D036226:Seedlings / Q000254:growth & development / Q000821:virology
chemical_list: D004202:Disinfectants; D010937:Plant Growth Regulators; D008627:Mercuric Chloride