[Interaction between insulin with liposome].
OBJECTIVE
To study the characteristics of the interaction between insulin and liposome.


METHODS
The interaction between insulin and liposome was studied by fluorescence spectra and microcalorimetry methods. The sample of insulin liposome interaction after separation by supper-centrifugalization or gel filtration was determined by fluorescence and HPLC.


RESULTS
The results indicate that there was only little increase in fluorescence intensity and no blue shift of peak in fluorescence spectrum. Fluorescence quenching experiments with NaI and acrylamide as quenchers showed that the KSVs (the slope of Strm-Volmer equation) of insulin were more similar to that with added liposome, indicating low interaction between insulin with liposome. The microcalorimetric results indicate that the heat released during the mixture of liposome with insulin, was 1.98 kcal.mol-1, suggesting that the reaction belongs to weak reaction. The quantity of insulin in the insulin-liposome mixture sample after separation by ultracentrifuge or by Sephadex G-75 determined by HPLC, the combination percent was only 0.2%, indicating low interaction between insulin and liposome.


CONCLUSIONS
The interaction between insulin and liposome was weak.
Zhang
Xuan
X
School of Pharmaceutical Science, Peking University, Beijing 100083, China.
Qi
Xian-rong
XR
Zhang
Qiang
Q
D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D004337:Drug Carriers; D016503:Drug Delivery Systems; D007004:Hypoglycemic Agents / Q000008:administration & dosage* / Q000737:chemistry; D007328:Insulin / Q000008:administration & dosage* / Q000737:chemistry; D008081:Liposomes; D013050:Spectrometry, Fluorescence
D004337:Drug Carriers; D007004:Hypoglycemic Agents; D007328:Insulin; D008081:Liposomes