title: Increased albumin plasma efflux contributes to hypoalbuminemia only during early phase of sepsis in rats.
abstract: The mechanisms leading to hypoalbuminemia in sepsis were explored by measuring plasma volume, albumin distribution, plasma albumin transcapillary escape rate (TER), and efflux (TER x albumin intravascular pool). These parameters were quantified in infected rats, injected intravenously with live Escherichia coli, and pair-fed and well-fed rats using an injection of (35)S-albumin and measuring plasma and whole body albumin concentrations. Animals were studied on days 1, 6, and 10 after infection. In pair-fed rats, neither albumin distribution nor exchange rate between the intra- and extravascular compartments was modified. The increase of plasma volume after infection partly explained hypoalbuminemia. Infection resulted in a reduction of the total albumin pool of the body all along the experimental period, indicating a net loss of the protein. Albumin TER (%/day) was significantly increased 1 and 6 days after infection, but the absolute efflux was increased only on day 1. Normal values were observed on day 10. Therefore, an accelerated plasma efflux contributes to hypoalbuminemia only during the early period of sepsis. During this phase, the protein was retained in the extravascular space where it was probably catabolized. Later on, other factors are probably involved.
authors.affiliation: Centre de Recherche en Nutrition Humaine d'Auvergne and Unité de Nutrition et Métabolisme Protéique, Institut National de la Recherche Agronomique Theix, 63 122 Saint Genès Champanelle, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Ruot
authors.forename: Isabelle
authors.initials: I
authors.lastname: Papet
authors.forename: Fabienne
authors.initials: F
authors.lastname: Bechereau
authors.forename: Philippe
authors.initials: P
authors.lastname: Denis
authors.forename: Caroline
authors.initials: C
authors.lastname: Buffiere
authors.forename: Johan
authors.initials: J
authors.lastname: Gimonet
authors.forename: Francoise
authors.initials: F
authors.lastname: Glomot
authors.forename: Mimoun
authors.initials: M
authors.lastname: Elyousfi
authors.forename: Denis
authors.initials: D
authors.lastname: Breuille
authors.forename: Christiane
authors.initials: C
authors.lastname: Obled
mesh_terms: D000818:Animals; D001810:Blood Volume; D002199:Capillary Permeability; D004927:Escherichia coli Infections / Q000378:metabolism* / Q000503:physiopathology; D034141:Hypoalbuminemia / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D012709:Serum Albumin