Establishment and characterization of cancer cell cultures and xenografts derived from primary or metastatic Mullerian cancers.
OBJECTIVE
The purpose of this study was to characterize cell cultures and xenografts derived from patients with ovarian cancer.


METHODS
Ninety specimens from 67 patients were plated in RPMI 1640 or inoculated in nude mice. Growth characteristics of cell cultures and xenografts were determined. Expression of receptors for estrogen, progesterone, androgen, epithelial growth factor, fibroblast growth factor, HER-2/erbB-2/c-neu proto-oncogene, and the P53 expression were characterized by immunocytochemistry in 28 cell cultures.


RESULTS
Forty-nine percent of samples were cultured successfully in vitro. Ascitic and pleural effusion specimens were more likely to produce a cell culture or a xenograft than solid tissue specimens (P < 0.005). All of the cell cultures had an epithelial morphology, and 89% were aneuploid with a mean DNA index of 1.6 (range, 0.9-3.0). Of 54 and 61 specimens inoculated into nude mice i.p. and s.c., 15 (28%) and 18 (30%) produced a xenograft, respectively, with two-thirds of these xenografts being reproducibly tumorigenic. The median time to first passage was 21 weeks for cell cultures and 8-12 weeks for xenografts. Expression of epithelial growth factor receptor, HER-2/erbB-2/c-neu proto-oncogene, fibroblast growth factor receptor, estrogen, progesterone, and androgen was seen in 24, 21, 31, 17, 43, and 18%, respectively. P53 was overexpressed in 62% of cell cultures analyzed.


CONCLUSIONS
Ovarian cancer cells collected from effusions are easier to grow in vitro than in vivo. The only characteristic that may be associated with tumorigenicity was abnormal P53 expression. This panel of ovarian cancer materials provides useful models for biological or therapeutical studies.
Verschraegen
Claire F
CF
Department of Gynecologic Medical Oncology, The University of Texas, M.D. Anderson Cancer Center, Houston, Texas 77030, USA. cverschraegen@salud.unm.edu
Hu
Wei
W
Du
Yu
Y
Mendoza
John
J
Early
Janet
J
Deavers
Michael
M
Freedman
Ralph S
RS
Bast
Robert C
RC
Kudelka
Andrzej P
AP
Kavanagh
John J
JJ
Giovanella
Beppino C
BC
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D018929:Cell Culture Techniques / Q000379:methods; D002453:Cell Cycle; D066246:ErbB Receptors / Q000032:analysis; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D018200:Mixed Tumor, Mullerian / Q000473:pathology*; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000473:pathology*; D000090063:Proto-Oncogene Mas; D011987:Receptors, Steroid / Q000032:analysis; D014183:Transplantation, Heterologous / Q000379:methods; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011987:Receptors, Steroid; D066246:ErbB Receptors