title: Rabbit calvarial wound healing by means of seeded Caprotite scaffolds.
abstract: Autologous bone is the most successful bone-grafting material; however, limited supply and donor site morbidity are problematic. Synthetic bone substitutes are effective, but healing is slow and unpredictable. Osseous wound healing may be enhanced if bone substitutes are combined with autologous bone marrow cells. To test this hypothesis, we created 40 calvarial defects in 20 12-week-old New Zealand White rabbits, divided into four groups: (1) unrepaired controls, (2) autologous bone grafts, (3) unseeded Caprotite (a polymer-ceramic composite) grafts, and (4) Caprotite grafts seeded with autologous bone marrow stromal cells. CT scans were obtained at 0, 6, and 12 weeks post-operatively, and defects were harvested for histology. Defects repaired with autologous bone had significantly (p < 0.05) more bone than the other three groups, although seeded Caprotite defects showed different wound-healing sequelae. Results suggest that seeded Caprotite scaffolds did not significantly enhance osseous defect healing compared with controls.
authors.affiliation: Plastic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USA.
authors.forename: S M S
authors.initials: SM
authors.lastname: Bidic
authors.forename: J W
authors.initials: JW
authors.lastname: Calvert
authors.forename: K
authors.initials: K
authors.lastname: Marra
authors.forename: P
authors.initials: P
authors.lastname: Kumta
authors.forename: P
authors.initials: P
authors.lastname: Campbell
authors.forename: R
authors.initials: R
authors.lastname: Mitchell
authors.forename: W
authors.initials: W
authors.lastname: Wigginton
authors.forename: J O
authors.initials: JO
authors.lastname: Hollinger
authors.forename: L
authors.initials: L
authors.lastname: Weiss
authors.forename: M P
authors.initials: MP
authors.lastname: Mooney
mesh_terms: D020341:Absorbable Implants; D000704:Analysis of Variance; D000818:Animals; D016026:Bone Marrow Transplantation; D001861:Bone Regeneration; D018786:Bone Substitutes; D016025:Bone Transplantation; D020007:Implants, Experimental; D008297:Male; D023421:Models, Animal; D010294:Parietal Bone / Q000601:surgery; D011817:Rabbits; D018709:Statistics, Nonparametric; D023822:Tissue Engineering; D014945:Wound Healing
chemical_list: D018786:Bone Substitutes