title: [Long-term immunogenicity and immunologic priming of animals to pulsed controlled-release system for tetanus toxoid vaccine delivery].
abstract: OBJECTIVE
  To evaluate the immunogenicity of tetanus toxoid (TT) encapsulated in biodegradable polymer microspheres composed of polylactide (PLA).
  
  
  METHODS
  The antibody levels elicited by microsphere formulations in mice for 1 year were examined, the anamnestic responses to a low dose booster 1 year after priming were also examined.
  
  
  RESULTS
  Microsphere formulations made from PLA were characterized by pulse controlled-release models, differing in polymer molecular weight, protein loading and particle size of the microspheres. Microsphere formulations elicited significantly higher IgG antibody levels than a single injection of soluble TT. The antibody levels elicited by microsphere formulations were similar to those elicited by three doses of soluble TT.
  
  
  CONCLUSIONS
  A single-dose tetanus toxoid based on pulsed release from biodegradable and biocompatible polymer microspheres has been developed. The formulations showed great benefits and pharmaceutical application.
authors.affiliation: School of Pharmaceutical Sciences, Peking University, Beijing 100083, China. heying@mail.bjmu.edu.cn
authors.forename: Y
authors.initials: Y
authors.lastname: He
authors.forename: Z H
authors.initials: ZH
authors.lastname: Chen
authors.forename: S L
authors.initials: SL
authors.lastname: Wei
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D005260:Female; D007070:Immunoglobulin A / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D011091:Polyesters; D013745:Tetanus Toxoid / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D007070:Immunoglobulin A; D011091:Polyesters; D013745:Tetanus Toxoid; C033616:poly(lactide)