[Expression of recombinant HIV-1 envelope glycoprotein gp41].
OBJECTIVE
To construct and express HIV-1 env gp41 gene for develoing a simple and rapid test for HIV-1 infection.


METHODS
HIV-1 env gp41 gene of BH10 strain (nt6,977-7,497) was constructed into expressing vector pBV221 and expressed in E. Coli HB101. The expressed proteins were purified on 15% SDS-PAGE, the specific protein gel was cut down, transferred onto nitrocellulose membrane and stained with ponceau for 10 minutes. The membrane was detected with positive and negative serum respectively. The membrane was blocked with blocking buffer and cut into 2 mm of each strip and fixed into the well of thin plastic plate.


RESULTS
We obtained a strand plasmid expressing HIV-1 env gene and the protein.


CONCLUSIONS
The results showed that: (1) HIV-1 env gp41 protein can be used to detect HIV-1 antibody in serum of individual; (2) The expressed protein is a nonfusion protein and has high specificity and sensitivity to HIV-1.
Teng
Z
Z
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100050.
Li
D
D
Gao
L
L
D000956:Antigens, Viral / Q000096:biosynthesis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015700:HIV Envelope Protein gp41 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011995:Recombination, Genetic
D000956:Antigens, Viral; D015700:HIV Envelope Protein gp41; D012333:RNA, Messenger