[Relationship between complement activation and reactive oxygen species generation in inflammatory response].
OBJECTIVE
To verify the proposed activation feedback mechanism between complement and polymorphonuclear leukocytes(PMN) derived reactive oxygen species(ROS).


METHODS
In vitro tests were designed to identify the activation feedback process by alternative activations of complement by inulin or PMNs by 12-O-tetradecanoylphorbol-13-acetate(TPA) in the test medium in consisting serum complement and isolated PMNs, with chemiluminescence technique to determine the production of ROS and complement fixation trial for complement activations.


RESULTS
Evidently that activated complement could activate PMNs to release ROS which further activated complement, so the activating feedback cycle was set.


CONCLUSIONS
The complement and PMNs-ROS activation feedback cycle mechanism was recognized. It was expected to serve for delineating the regulation of inflammatory response.
Fan
X
X
Department of Pathology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Ling
Y
Y
Wu
Y
Y
Hou
J
J
D000818:Animals; D002469:Cell Separation; D003167:Complement Activation / Q000187:drug effects / Q000502:physiology*; D006224:Cricetinae; D007249:Inflammation / Q000209:etiology; D007444:Inulin / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism* / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D017382:Reactive Oxygen Species; D007444:Inulin; D013755:Tetradecanoylphorbol Acetate