[Study on GMA-DNA adducts in vivo].
OBJECTIVE
To investigate whether glycidly mechacrylate (GMA-DNA) adducts can be produced in various organs of rat in vivo.


METHODS
Rats divided into 5 groups were orally administrated with mutagen, GMA 31.25, 62.5, 125, 250 mg/kg respectively for 14 days. DNA adducts produced in liver, kidney, white blood cells and testis had been analyzed by nuclease P1 mediated 32P-postlabelling method.


RESULTS
Several GMA-DNA adducts were formed in various organs (in white blood cells, 4 types, liver and kidney, 3 types and testis 1 type). The amount of GMA-DNA adducts increased with GMA dosage within 0-125 mg/kg dosages, degree of the overall level of GMA-DNA adducts in various organs were kidney > liver > white blood cells > testis. N3-methacrylate-2-hdroxypropyl-deoxycytidine monophosphate was found in kidney, liver and white blood cells.


CONCLUSIONS
GMA with electrophilic group could react with negatively charged centers on DNA and form GMA-DNA adducts.
Tan
M
M
Institute of Occupational Medicine, Chinese Academy of Preventive Medicine, Beijing 100050.
Xu
J
J
Li
Z
Z
Lei
H
H
Zuo
J
J
D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D018736:DNA Adducts / Q000096:biosynthesis*; D004249:DNA Damage / Q000187:drug effects; D003843:Deoxycytidine Monophosphate / Q000031:analogs & derivatives / Q000096:biosynthesis; D004852:Epoxy Compounds / Q000633:toxicity*; D007668:Kidney / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008689:Methacrylates / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000378:metabolism
D002273:Carcinogens; D018736:DNA Adducts; D004852:Epoxy Compounds; D008689:Methacrylates; C477758:N3-methacrylate-2-hydroxypropyl-deoxycytidine monophosphate; D003843:Deoxycytidine Monophosphate; C007870:glycidyl methacrylate