Rapid generation of inducible mouse mutants.
We have generated an optimized inducible recombination system for conditional gene targeting based on a Cre recombinase-steroid receptor fusion. This configuration allows efficient Cre-mediated recombination in most organs of the mouse upon induction, without detectable background activity. An ES cell line, was established that carries the inducible recombinase and a loxP-flanked lacZ reporter gene. Out of this line, completely ES cell-derived mice were efficiently produced through tetraploid blastocyst complementation, without the requirement of mouse breeding. Our findings provide a new concept allowing the generation of inducible mouse mutants within 6 months, as compared to 14 months using the current protocol.
Seibler
Jost
J
ARTEMIS Pharmaceuticals GmbH, Neurather Ring 1, 51063 Cologne, Germany.
Zevnik
Branko
B
Küter-Luks
Birgit
B
Andreas
Susanne
S
Kern
Heidrun
H
Hennek
Thomas
T
Rode
Anja
A
Heimann
Cornelia
C
Faust
Nicole
N
Kauselmann
Gunther
G
Schoor
Michael
M
Jaenisch
Rudolf
R
Rajewsky
Klaus
K
Kühn
Ralf
R
Schwenk
Frieder
F
D000818:Animals; D002460:Cell Line; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics*; D008822:Mice, Transgenic; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D011987:Receptors, Steroid; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases