[Study on preparation and tissue distribution of SEA liposomes].
OBJECTIVE
To make a study on the preparation and tissue distribution of staphylococcal enterotoxin A (SEA) liposomes and to provide scientific basis for the therapy of liver cancer by using SEA liposomes.


METHODS
SEA liposomes were prepared by reverse-phase evaporation; the diameter and entrapment efficiency (EC) of SEA liposomes were determined. 125I labeled SEA solution and 125I-SEA liposomes were administrated intravenously to mice, respectively. The radioactivity of the organs was determined by gamma-counter.


RESULTS
The mean diameter and EC of SEA liposomes were 505 +/- 34 nm and 44.1% +/- 4.8%, respectively. SEA liposomes were found mainly distributed in the liver and spleen. SEA liposomes had a higher blood clearance, compared with SEA solution; SEA solution had high-radio-activity in plasma and kidney; there was statistical significance between the two groups (P < 0.05).


CONCLUSIONS
The preparation method of SEA liposomes is simple and repeatable. SEA liposomes possess liver-targeting properties and may provide a new application foreground for the treatment of liver cancer.
Li
Zhiyu
Z
Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
He
Sheng
S
Xue
Hua
H
Su
Min
M
Wang
Xian
X
D000818:Animals; D004337:Drug Carriers; D016503:Drug Delivery Systems / Q000379:methods; D004768:Enterotoxins / Q000008:administration & dosage* / Q000493:pharmacokinetics; D005260:Female; D008081:Liposomes; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D011897:Random Allocation; D014018:Tissue Distribution
D004337:Drug Carriers; D004768:Enterotoxins; D008081:Liposomes; C026066:enterotoxin A, Staphylococcal