Aminopeptidase C of Aspergillus niger is a novel phenylalanine aminopeptidase.
A novel enzyme with a specific phenylalanine aminopeptidase activity (ApsC) from Aspergillus niger (CBS 120.49) has been characterized. The derived amino acid sequence is not similar to any previously characterized aminopeptidase sequence but does share similarity with some mammalian acyl-peptide hydrolase sequences. ApsC was found to be most active towards phenylalanine beta-naphthylamide (F-beta NA) and phenylalanine para-nitroanilide (F-pNA), but it also displayed activity towards other amino acids with aromatic side chains coupled to beta NA; other amino acids with non-aromatic side chains coupled to either pNA or beta NA were not hydrolyzed or were poorly hydrolyzed. ApsC was not able to hydrolyze N-acetylalanine-pNA, a substrate for acyl-peptide hydrolases.
Basten
Daniëlle E J W
DE
Laboratory of Microbiology, Wageningen University, 6703 HA Wageningen, The Netherlands.
Dekker
Peter J T
PJ
Schaap
Peter J
PJ
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D010649:Phenylalanine / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity
D010649:Phenylalanine; D000626:Aminopeptidases; C072222:aminopeptidase C; C049035:phenylalanine aminopeptidase