Impala, a transposon from Fusarium oxysporum, is active in the genome of Penicillium griseoroseum.
An autonomous impala transposon trapped in Fusarium oxysporum by insertion within the niaD gene encoding nitrate reductase was introduced in the genome of the fungus Penicillium griseoroseum, a producer of pectinase enzymes. Through a phenotypic assay, we demonstrate that this element is able to excise from the niaD gene and to reinsert at new genomic positions. As in the original host, impala inserts into a TA site and footprints left by impala excisions are generally 5 bp. The fact that impala is able to transpose in P. griseoroseum offers the opportunity to develop a gene-tagging system based on this element with the objective to detect and clone genes related in pectinase production.
de Queiroz
Marisa Vieira
MV
Departamento de Microbiologia, Universidade Federal de Viçosa, 36571-000, MG, Viçosa, Brazil. mvqueiro@mail.ufv.br
Daboussi
Marie Josée
MJ
D015139:Blotting, Southern; D004251:DNA Transposable Elements / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D005670:Fusarium / Q000235:genetics*; D016681:Genome, Fungal; D010407:Penicillium / Q000235:genetics*
D004251:DNA Transposable Elements; D004271:DNA, Fungal