[Sequencing of cytochrome C oxidase 1 gene of Ancylostoma duodenale and Necator americanus].
OBJECTIVE
To identify the genetic diversity between Ancylostoma duodenale and Necator americanus.


METHODS
Mitochondrial cytochrome C oxidase subunit 1 (CO1) gene was amplified from genomic DNA of human hookworms collected from infected patients in Hejiang County, Sichuan Province, and the purified PCR products were directly sequenced by using Licor auto-sequencer.


RESULTS
The PCR products were about 700 bp. Alignment of CO1 gene fragment sequences showed 89.7% similarity between Ancylostoma duodenale and Necator americanus, but still certain nucleotide variations (10.3%) existed.


CONCLUSIONS
CO1 gene sequence can be used as a marker to identify the two species of human hookworms.
Li
T
T
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Zhan
B
B
Hawdon
J M
JM
Gong
X
X
Xiao
S
S
Shan
Q
Q
Feng
Z
Z
Hotez
P J
PJ
D000595:Amino Acid Sequence; D000722:Ancylostoma / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D003578:Cytochrome-c Peroxidase / Q000737:chemistry / Q000235:genetics*; D018509:DNA, Helminth / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D017221:Necator americanus / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D018509:DNA, Helminth; D003578:Cytochrome-c Peroxidase