[Construction of DNA vaccine including a chimeric gene encoding Cysticercus cellulosae antigen and porcine interleukin-4].
OBJECTIVE
To construct a fusion expression vector for DNA vaccine including porcine interleukin-4(IL-4) and antigen cC1 to enhance the protective immunity of Cysticercus cellulosae antigen cC1.


METHODS
The cDNA fragments encoding porcine IL-4 and cC1 were amplified respectively by PCR and the fused. The obtained chimeric gene IL-4cC1 contained a synthetic linker of ten amino acids and the sequence surrounding its 5' AUG initiatory codon was changed to optimized translational initiation.


RESULTS
Identified by restriction enzyme analysis, an insert fragment of 1.5 kb was demonstrated. It had the same sequence as reported and designed by DNA sequencing analysis.


CONCLUSIONS
A fusion expression plasmid containing porcine IL-4 and cC1 was constructed.
Wu
D
D
Department of Medical Genetics, Second Military Medical University, Shanghai 200433.
Guo
Y
Y
Sun
S
S
D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics*; D019677:Artificial Gene Fusion; D003552:Cysticercus / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D015847:Interleukin-4 / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics; D013552:Swine; D019444:Vaccines, DNA / Q000235:genetics*
D000947:Antigens, Helminth; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D015847:Interleukin-4