title: [Effect of bushen yizhi recipe on neurotransmitter release in A beta segment neurotoxin induced NG108-15 cellular model of Alzheimer disease].
abstract: OBJECTIVE
  To observe the effect of Bushen Yizhi Recipe (BSYZR) on neurotransmitter release in A beta segment neurotoxin induced NG108-15 cellular model of Alzheimer disease (AD).
  
  
  METHODS
  The levels of choline acetyltransferase (ChAT) activity, synapsin and functional synapse formation rate in the cellular model treated with BSYZR containing serum were determined by Western blot analysis, immunoradiometric assay and electrophysiologic technique.
  
  
  RESULTS
  BSYZR containing serum treatment could cause increase of ChAT activity and synapsin level in model cells, as compared with those in normal control model cells treated with non-drug containing serum, it also could regulate the release capacity of transmitter and raise the functional synapse formation.
  
  
  CONCLUSIONS
  BSYZR could reduce the reaction of cell to A beta neurotoxin, indicating that it could be antagonistic to the pathological development of AD by means of raising the neurotransmitter release capacity.
authors.affiliation: Institute of Neuroscience, 2nd Affiliated Hospital of Guangxi College of TCM, Nanning 530001.
authors.forename: Zhen-guo
authors.initials: ZG
authors.lastname: Zhong
authors.forename: Mao-cai
authors.initials: MC
authors.lastname: Liu
authors.forename: Shi-long
authors.initials: SL
authors.lastname: Lai
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D009498:Neurotoxins / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D016704:Synapsins / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D004365:Drugs, Chinese Herbal; D009498:Neurotoxins; D018377:Neurotransmitter Agents; D016704:Synapsins; D002795:Choline O-Acetyltransferase