Identification of a mouse orthologue of the CED-6 gene of Caenorhabditis elegans.
The rapid engulfment of apoptotic cells is a specialized innate immune response used by organisms to remove apoptotic cells. In mammals, several receptors that recognize apoptotic cells have been identified. Previous analysis of the engulfment gene ced-6 in Caenorhabditis elegans (C. elegans) has suggested that CED-6 is an adapter protein that participates in signal transduction pathway that mediates the specific recognition and engulfment of apoptotic cells. Here, we describe our isolation and partial characterization of a mouse cDNA, which is like an orthologue of C. elegans CED-6. PCR screening of mouse cDNA pool with primers designed from the C. elegans CED-6 cDNA sequence resulted in about 300 bp PCR product which was partially sequenced and then screened to a mouse full-length cDNA library. Thus in this study we report the identification of a novel C. elegans CED-6-like orthologue in mouse, which has probable apoptotic like function.
Banerjee
H
H
Department of Biology, Elizabeth City State University under University of North Carolina, Campus Box 930, Elizabeth City, NC 27909, USA. bhirendranath@mail.ecsu.edu
Hawkins
Z
Z
Johnson
T
T
Eley
S
S
Alikhan
A
A
Mcdaniel
M
M
Singh
I
I
Raymond
J
J
D000818:Animals; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D003001:Cloning, Molecular; D051379:Mice; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D051017:Apoptosis Regulatory Proteins; C112888:CED-6 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D010750:Phosphoproteins