title: Infertility and aneuploidy in mice lacking a type IA DNA topoisomerase III beta.
abstract: We report that disruption of the mouse TOP3 beta gene encoding DNA topoisomerase III beta, one of the two mammalian type IA DNA topoisomerases, leads to a progressive reduction in fecundity. The litter size in crosses of top3 beta(-/-) mice decreases over time and through successive generations, and this decrease seems to reflect embryonic death rather than impaired fertilization. These observations are suggestive of a gradual accumulation of chromosomal defects in germ cells lacking DNA topoisomerase III beta, and this interpretation is supported by the observation of a high incidence of aneuploidy in the spermatocytes of infertile top3 beta(-/-) males. Cytogenetic examination of spermatocytes of wild-type mice also indicates that DNA topoisomerase III beta becomes prominently associated with the asynaptic regions of the XY bivalents during pachytene, and that there is a time lag between the appearance of chromosome-bound DNA topoisomerase III beta and Rad51, a protein known to be involved in an early step of homologous recombination. We interpret these findings, together with the known mechanistic characteristics of different subfamilies of DNA topoisomerases, in terms of a specific role of a type IA DNA topoisomerase in the resolution of meiotic double-Holliday junctions without crossing over. This interpretation is most likely applicable to mitotic cells as well and can explain the universal presence of at least one type IA DNA topoisomerase in all organisms.
authors.affiliation: Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA.
authors.forename: Kelvin Y
authors.initials: KY
authors.lastname: Kwan
authors.forename: Peter B
authors.initials: PB
authors.lastname: Moens
authors.forename: James C
authors.initials: JC
authors.lastname: Wang
mesh_terms: D000782:Aneuploidy; D000818:Animals; D002869:Chromosome Aberrations; D003433:Crosses, Genetic; D004264:DNA Topoisomerases, Type I / Q000235:genetics* / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D005260:Female; D005838:Genotype; D017404:In Situ Hybridization, Fluorescence; D007246:Infertility; D007527:Isoenzymes / Q000235:genetics* / Q000502:physiology*; D008297:Male; D008540:Meiosis; D008677:Metaphase; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D051135:Rad51 Recombinase; D011995:Recombination, Genetic; D013090:Spermatocytes / Q000166:cytology; D013997:Time Factors
chemical_list: D004268:DNA-Binding Proteins; D007527:Isoenzymes; D051135:Rad51 Recombinase; C495154:Rad51 protein, mouse; D004264:DNA Topoisomerases, Type I