title: Use of meristem tip culture to eliminate carnation latent virus from carnation plants.
abstract: A successful protocol for meristem tip culture to eliminate carnation latent virus from carnation cv. scania has been described . The virus was found to be mechanically transmissible to Chenopodium quinoa, C. amaranticolor, Dianthus barbatus and Saponaria vaccaria. Murashige and Skoog'smedium (MS) supplemented with NAA (1.0 microM) and Kn (20.0 microM) proved best for meristem establishment and microshoots were rooted in MS medium supplemented with IBA (5.0 microM). Meristems measuring 0.1 and-0.2 mm yielded virus free plants and larger meristems were not effective.
authors.affiliation: Department of Biotechnology, Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan 173 230, India. Manishamangal@rediffmail.com
authors.forename: Manisha
authors.initials: M
authors.lastname: Mangal
authors.forename: S V
authors.initials: SV
authors.lastname: Bhardwaj
authors.forename: D R
authors.initials: DR
authors.lastname: Sharma
authors.forename: R
authors.initials: R
authors.lastname: Kaur
authors.forename: A K
authors.initials: AK
authors.lastname: Mangal
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D017788:Carlavirus / Q000187:drug effects* / Q000254:growth & development; D029749:Dianthus / Q000737:chemistry* / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D007701:Kinetin; D018519:Meristem / Q000737:chemistry / Q000378:metabolism*; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000737:chemistry* / Q000821:virology; D018520:Plant Shoots / Q000737:chemistry / Q000821:virology; D012698:Serologic Tests
chemical_list: D000469:Alkaline Phosphatase; D000225:Adenine; D007701:Kinetin