title: [Random amplified polymorphic DNA analysis of Cryptosporidium species and strains].
abstract: OBJECTIVE
  To analyse the genetic heterogeneity of 8 isolates of Cryptosporidium belonging to three species (Cryptosporiduim muris, Cryptosporiduim parvum and Cryptosporidium meleagridis) using RAPD technique.
  
  
  METHODS
  DNA extracts of the 8 isolates were amplified by PCR using 26 out of 200 primers. The amplified products were separated by agar electrophoresis followed by staining with ethidium bromide.
  
  
  RESULTS
  RAPD analysis revealed; 1. Obvious differences among species were found, the average genetic distance being 7.037; 2. Certain differences among different isolates of the same species were found, the average genetic distance being 5.162.
  
  
  CONCLUSIONS
  RAPD could differentiate the common and respective gene fragments of the 8 isolates of Cryptosporidium.
authors.affiliation: Department of Preventive Veterinary Science, Quartermaster University of PLA, Changchun 130062.
authors.forename: Z C
authors.initials: ZC
authors.lastname: Tian
authors.forename: X C
authors.initials: XC
authors.lastname: Zhang
authors.forename: J M
authors.initials: JM
authors.lastname: Wang
authors.forename: J G
authors.initials: JG
authors.lastname: Yin
authors.forename: J H
authors.initials: JH
authors.lastname: Li
authors.forename: J B
authors.initials: JB
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics*; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis*; D004372:Ducks; D005243:Feces / Q000469:parasitology; D018740:Genetic Heterogeneity; D051379:Mice; D011817:Rabbits; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan