[Experimental study on effect of zenggu-I, -II, -III on osteoblast in vitro].
OBJECTIVE
To investigate the effect of Zenggu (ZG)-I, -II and -III on osteoblast (OB) cultured in vitro.


METHODS
OB was isolated from calvaria of 22-day-old fetal SD rats by means of modified sequential collagenase digestion and incubated in RM1640 medium and the cell morphology was observed by alkaline phosphatase (ALP) staining. Different concentrations of ZG-I, -II and -III were added to the OB and incubated. The effect of ZG on the proliferation and osteogenesis of OB was monitored by MTT analysis, BGP content radioimmunoassay, ALP activity and calcium contents determination.


RESULTS
The BGP, ALP, and calcium contents of the cultured OB cells as well as the cell proliferation were higher in ZG-III group than those in the other groups (P < 0.05) in a dose-dependent manner. It suggested that ZG-III could promote the proliferation and osteogenesis of OB, but ZG-I and ZG-II had insignificant influence on OB cultured in vitro.


CONCLUSIONS
ZG-III could promote the OB proliferation and bone formation, ZG-I, -II, -III sequential therapy could effectively prevent and treat osteoporosis and promote the bone formation.
Fu
G
G
Union Hospilal, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430022.
Wei
Y L
YL
Liang
K Y
KY
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005333:Fetus; D031299:Mucuna / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000166:cytology
D004365:Drugs, Chinese Herbal