Efficient production and capture of 8-prenylnaringenin and leachianone G-biosynthetic intermediates of sophoraflavanone G--by the addition of cork tissue to cell suspension cultures of Sophora flavescens.
It has previously been demonstrated that the addition of cork tissue to cell suspension cultures of Sophora flavescens stimulates the production of sophoraflavanone G, most of which has been recovered from the added cork tissue. In the present study, it was found that two precursors of sophoraflavanone G, 8-prenylnaringenin (sophoraflavanone B) and leachianone G, both of which have never been detected either in cultured cells or in the original plants, also accumulated in the added cork tissue. Thirteen minor flavonoids including three prenylated flavonoids, in addition to 8-prenylnaringenin and leachianone G, were isolated from the cork tissue co-incubated with S. flavescens cells. The new compounds flavescenones A, B and C, were determined to be (3R)-5, 7, 2'-trihydroxy-6-gamma, gamma-dimethylallyl-4', 5'-methylenedioxyisoflavanone; 5, 7, 2'-trihydroxy-6-gamma, gamma-dimethylallyl-4', 5'-methylenedioxyisoflavone and 2-[2',4'-dihydroxy-3'-(gamma-hydroxymethyl-gamma-methylallyl)phenyl]-5,6-methylenedioxybenzofuran, respectively, by means of spectroscopic analyses that included 2D-NMR techniques.
Zhao
Ping
P
Medicinal Plant Garden, School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
Hamada
Chie
C
Inoue
Kenichiro
K
Yamamoto
Hirobumi
H
D000085:Acetates / Q000494:pharmacology; D002478:Cells, Cultured; D002850:Chromatography, Gel / Q000379:methods; D003517:Cyclopentanes / Q000494:pharmacology; D044950:Flavanones; D005419:Flavonoids / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D054883:Oxylipins; D017368:Protein Prenylation; D029963:Quercus; D029909:Sophora / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D014934:Wood
C119737:8-prenylnaringenin; D000085:Acetates; D003517:Cyclopentanes; D044950:Flavanones; D005419:Flavonoids; D054883:Oxylipins; C474046:leachianone G; C072239:methyl jasmonate