Detection of hilA gene sequences in serovars of Salmonella enterica subspecies enterica.
hilA gene promoter, component of the Salmonella Pathogenicity Island 1, has been found in Salmonella serovar Typhimurium, being important for the regulation of type III secretion apparatus genes. We detected hilA gene sequences in Salmonella serovars Typhi, Enteritidis, Choleraesuis, Paratyphi A and B, and Pullorum, by polymerase chain reaction (PCR) and hybridization techniques. The primers to carry out PCR were designed according to hilA sequence. A low stringency hybridization with the probe pVV441 (hilA open-reading-frame plasmid) was carried out. To find hilA gene sequences in other Salmonella sp. suggest that these serovars could have similar sequences of this kind of virulence genes.
Cardona-Castro
Nora
N
Laboratorio de Microbiología, Instituto Colombiano de Medicina Tropical, Sabaneta, Antioquia, Colombia. icmt@epm-net.co
Restrepo-Pineda
Eliana
E
Correa-Ochoa
Margarita
M
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D006824:Hybridization, Genetic; D016133:Polymerase Chain Reaction; D019779:Salmonella enterica / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*
D001426:Bacterial Proteins; C102236:HilA protein, Salmonella; D015534:Trans-Activators