title: Ginsenoside Rc and Re stimulate c-fos expression in MCF-7 human breast carcinoma cells.
abstract: We have found that ginsenoside Rc and Re induce c-fos in MCF-7 human breast carcinoma cells at both the mRNA and protein levels. However, neither ginsenoside activated the expression of reporter gene under the control of AP-1/TPA response elements. We have also examined the possibility that ginsenoside Rc and Re act by binding to intracellular steroid hormone receptors that act as transcriptional factors in the nucleus in inducing c-fos mRNA in MCF7 human breast carcinoma cells. However, ginsenoside Rc and Re did not bind to glucocorticoid, androgen, estrogen, or retinoic acid receptors as examined by the transcription activation of the luciferase reporter genes in CV-1 cells that were transiently transfected with the corresponding steroid hormone receptors and hormone responsive luciferase reporter plasmids. These data demonstrate that ginsenoside Rc and Re act via other transcription factors and not via estrogen receptor in c-Fos expression.
authors.affiliation: College of Engineering, Institute of Biotechnology, Department of Bioscience and Biotechnology, Sejong University, Seoul, Korea. yjlee@sejong.ac.kr
authors.forename: Young Joo
authors.initials: YJ
authors.lastname: Lee
authors.forename: Young Ran
authors.initials: YR
authors.lastname: Jin
authors.forename: Won Chung
authors.initials: WC
authors.lastname: Lim
authors.forename: Sang Mi
authors.initials: SM
authors.lastname: Ji
authors.forename: Jung Yoon
authors.initials: JY
authors.lastname: Cho
authors.forename: Jae Jun
authors.initials: JJ
authors.lastname: Ban
authors.forename: Seung Ki
authors.initials: SK
authors.lastname: Lee
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism*; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D016762:Genes, fos / Q000187:drug effects* / Q000502:physiology; D036145:Ginsenosides / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
chemical_list: D036145:Ginsenosides; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; C044462:ginsenoside Rc; C049864:ginsenoside Re