[Long-term immunogenicity and immunologic priming of animals to pulsed controlled-release system for tetanus toxoid vaccine delivery].
OBJECTIVE
To evaluate the immunogenicity of tetanus toxoid (TT) encapsulated in biodegradable polymer microspheres composed of polylactide (PLA).


METHODS
The antibody levels elicited by microsphere formulations in mice for 1 year were examined, the anamnestic responses to a low dose booster 1 year after priming were also examined.


RESULTS
Microsphere formulations made from PLA were characterized by pulse controlled-release models, differing in polymer molecular weight, protein loading and particle size of the microspheres. Microsphere formulations elicited significantly higher IgG antibody levels than a single injection of soluble TT. The antibody levels elicited by microsphere formulations were similar to those elicited by three doses of soluble TT.


CONCLUSIONS
A single-dose tetanus toxoid based on pulsed release from biodegradable and biocompatible polymer microspheres has been developed. The formulations showed great benefits and pharmaceutical application.
He
Y
Y
School of Pharmaceutical Sciences, Peking University, Beijing 100083, China. heying@mail.bjmu.edu.cn
Chen
Z H
ZH
Wei
S L
SL
D000818:Animals; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D005260:Female; D007070:Immunoglobulin A / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D011091:Polyesters; D013745:Tetanus Toxoid / Q000008:administration & dosage* / Q000276:immunology
D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D007070:Immunoglobulin A; D011091:Polyesters; D013745:Tetanus Toxoid; C033616:poly(lactide)