Trypan blue not toxic for retinal pigment epithelium in vitro.
OBJECTIVE
To investigate whether trypan blue has a toxic effect on cultured retinal pigment epithelial (retinal pigment epithelium) cells.


METHODS
Experimental study with a direct live/dead cell staining technique using fluorescent dyes.


METHODS
Cultured human retinal pigment epithelium cells were exposed for 5 minutes to various concentrations of trypan blue (0.06%, 0.15%, 0.30%), and cell viability was confocally measured.


RESULTS
No increased cell death was found in cultures incubated in any of the trypan blue concentrations used.


CONCLUSIONS
These findings indicate that a short exposure of trypan blue does not have a toxic effect on cultured retinal pigment epithelium cells.
Stalmans
Peter
P
Department of Ophthalmology, UZLeuven, Leuven, Belgium. Peter.Stalmans@uz.kuleuven.ac.be
Van Aken
Elisabeth H
EH
Melles
Gerrit
G
Veckeneer
Marc
M
Feron
Eric J
EJ
Stalmans
Ingeborg
I
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents / Q000494:pharmacology*; D006801:Humans; D018613:Microscopy, Confocal; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000187:drug effects*; D014343:Trypan Blue / Q000494:pharmacology*
D004396:Coloring Agents; D014343:Trypan Blue