Highly efficient transport of carboxyfluorescein diacetate succinimidyl ester into COS7 cells using human papillomavirus-like particles.
Human papillomavirus virus-like particles (VLPs) have recently been used to deliver genes into mammalian cells in vitro and in vivo. Here, we investigated whether VLPs may serve as an efficient carrier of low molecular weight compounds (e.g. hormones, vitamins, peptides etc.) into cells. COS7 cells were incubated with recombinant HPV-16L1/L2 VLPs labelled with the fluorescence dye carboxyfluorescein diacetate succinimidyl ester. Using flow cytometry, we demonstrate that labelled VLPs can specifically bind to the cell surface followed by their complete internalisation. Our results indicate that VLPs are promising vehicles for highly efficient delivery of low molecular weight compounds into cells.
Bergsdorf
Christian
C
Virofem Diagnostica GmbH, Rheingaustrasse 190-196, P.O. Box 3540, 65174 Wiesbaden, Germany.
Beyer
Carsten
C
Umansky
Viktor
V
Werr
Margaret
M
Sapp
Martin
M
D000818:Animals; D000914:Antibodies, Viral / Q000494:pharmacology; D001692:Biological Transport; D019556:COS Cells; D002213:Capsid / Q000276:immunology / Q000378:metabolism; D036022:Capsid Proteins; D005452:Fluoresceins / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D009856:Oncogene Proteins, Viral / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D013388:Succinimides / Q000378:metabolism*; D014771:Virion / Q000737:chemistry; D022701:Virosomes
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D000914:Antibodies, Viral; D036022:Capsid Proteins; D005452:Fluoresceins; D005456:Fluorescent Dyes; C063316:L2 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D013388:Succinimides; D022701:Virosomes; C063317:L1 protein, Human papillomavirus type 16