Identification of a minimal sequence required for activation of the tomato golden mosaic virus coat protein promoter in protoplasts.
Transient expression studies using a Nicotiana benthamiana suspension cell-derived protoplast system have identified a minimal sequence that is necessary and sufficient for activation of the tomato golden mosaic virus coat protein (CP) promoter by the viral TrAP protein (also called AL2). The sequence has a bipartite arrangement in which elements located between -125 to -107 and -96 to -60 from the transcription start site are both required for TrAP-mediated activation. One of the sequences (-96 to -60) also appears to interact with a repressor, as its deletion increases basal promoter activity in the absence of TrAP. That competition experiments using the -107 to -60 sequence to titrate the repressor also resulted in increased basal transcription is consistent with this idea. Thus, in a protoplast system which models mesophyll, regulation of the minimal CP promoter involves both activation and derepression by TrAP.
Sunter
Garry
G
Department of Molecular Genetics and Plant Biotechnology Center, The Ohio State University, Columbus 43210, USA.
Bisaro
David M
DM
D036022:Capsid Proteins / Q000235:genetics*; D002460:Cell Line; D017791:Geminiviridae / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D018551:Solanum lycopersicum / Q000821:virology*; D011401:Promoter Regions, Genetic; D011523:Protoplasts / Q000821:virology*; D012097:Repressor Proteins / Q000502:physiology; D015534:Trans-Activators / Q000502:physiology
D036022:Capsid Proteins; D012097:Repressor Proteins; D015534:Trans-Activators