[A study on biological characteristics of normal adult human nasal septal chondrocytes cultured in vitro].
OBJECTIVE
To study the characteristics of normal adult human nasal chondrocytes cultured in vitro.


METHODS
The adult human nasal septal chondrocytes were cultured in vitro. The changes of cellular morphology from primary to subculture were investigated; to observe the proliferation of chondrocytes by the growth curve and the survive rate of chondrocytes; and to determine the biologic synthesis of the chondrocytes by toluidine blue, alkaline phosphatase staining and type II collagen immunohistochemistry reaction. The survive rate of the nasal septal chondrocytes was observed after being frozen (-196 degrees C).


RESULTS
The morphology of the primary chondrocytes maintained the spherical shape, and some became polygonal shape after 4 days. Most of the chondrocytes transformed into the shape of the fibroblast cell since the fifth generation. The number of the chondrocytes (2th generation) after being cultured in vitro for 5 days was approximately 4 fold of that of the plating. The phenotype of the chondrocytes was maintained in the first 4 generation, the survive rate of the chondrocytes suscitating from being frozen (-196 degrees C) was 93%.


CONCLUSIONS
The normal adult nasal septal chondrocytes cultured in monolayer in vitro maintained the specific chondrocytes phenotype in the first 4 generation, the growth was normal, and could survive in the condition of deep-frozen.
Li
X S
XS
Department of Otolaryngology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282.
Huang
J Z
JZ
D000328:Adult; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D019902:Chondrocytes / Q000502:physiology*; D006801:Humans; D009300:Nasal Septum / Q000166:cytology*