title: Two major Spanish clones of penicillin-resistant Streptococcus pneumoniae in Portuguese isolates of clinical origin.
abstract: We studied the genetic relatedness of 47 Portuguese penicillin-resistant 9V and 23F Streptococcus pneumoniae of clinical origin, using pulsed-field gel electrophoresis (PFGE) and restriction fragment length polymorphism analysis. PFGE fingerprinting showed that 24 isolates of serotype 9V and 23 isolates of serotype 23F were variants of the Spain(9V)-3 and Spain(23F)-1 clones, respectively. Fingerprinting of pbp1a, pbp2b and pbp2x genes showed that all the penicillin-resistant clones gave similar HinfI restriction patterns. In this study, serotypes 9V and 23F have different clonal origins and identical PBP genotypes, suggesting a horizontal transfer of resistance. Visual and computer-assisted analysis of PFGE patterns correlated well (r = 0.983).
authors.affiliation: Antibiotic Resistance Unit, Centre of Bacteriology, National Institute of Health Dr. Ricardo Jorge, Av. Padre Cruz, 1649-016 Lisbon, Portugal. manuela.canica@insa.min.saude.pt
authors.forename: Manuela
authors.initials: M
authors.lastname: Caniça
authors.forename: Ricardo
authors.initials: R
authors.lastname: Dias
authors.forename: M Vitória
authors.initials: MV
authors.lastname: Vaz-Pato
authors.forename: Catarina
authors.initials: C
authors.lastname: Carvalho
mesh_terms: D019881:Aminoacyltransferases; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006602:Hexosyltransferases; D005810:Multigene Family / Q000235:genetics; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics / Q000378:metabolism; D010403:Penicillin Resistance; D046915:Penicillin-Binding Proteins; D010449:Peptide Mapping; D010458:Peptidyl Transferases; D011008:Pneumococcal Infections / Q000382:microbiology*; D011174:Portugal; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013030:Spain; D013296:Streptococcus pneumoniae / Q000187:drug effects* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D019881:Aminoacyltransferases; C478701:penicillin-binding protein 2b, Streptococcus; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase