title: [Research of Typing for HLA-A, -B on Cord Blood Lymphocytes]
abstract: Serological typing for HLA-A, -B has been used for a long time. Recently with the developing of molecular biology technologies, HLA-A, -B typing is now turning to genotyping methods. In our study, the capacity of PCR-SSP in solving problems in HLA-A, -B typing with serological methes was evaluated. With this aim the serological method was compared with PCR-SSP in 102 cord blood samples, and the results showed that 18.6% of 102 cord blood samples can't give a satisfactory detection, for 14 samples, give discrepant results with the 2 methods. It is mainly due to weak expression of HLA class I cord blood lymphocytes and the cross reaction of some antigens. About B 15 group, the further study was made, it was found that most of the B 15 splits is wrongly disassigned, especially among the B62-B75, B75/*1511(+)-B75/*1511(-), B46-*1511 antigens. It was concluded that DNA typing is more preferable than serological typing, about B 15 group, the subtyping or high resolution typing can be fulfilled at first in China.
authors.affiliation: Nanfang Hospital, The First Military Medical University, Guangzhou 510515, China.
authors.forename: Jiong-Cai
authors.initials: JC
authors.lastname: Lan
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.forename: Zhi-Mei
authors.initials: ZM
authors.lastname: Zhang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Cao
authors.forename: Rong
authors.initials: R
authors.lastname: Xia
authors.forename: Da-Lin
authors.initials: DL
authors.lastname: Wu
authors.forename: Tao
authors.initials: T
authors.lastname: Wu