Polymorphisms beta2-glycoprotein I: phospholipid binding and multimeric structure.
beta2-glycoprotein I is a phospholipid-binding protein of 326 amino acids and is found in plasma at a concentration of approximately 200 microg/ml. It has a sequence of positively charged amino acids located at the carboxy terminus that mediates anionic phospholipid binding. Two polymorphisms (306Cys-->Gly and 316Trp-->Ser) located at the phospholipid-binding site have been described. Homozygous state for either mutation and a compound heterozygous state show no phospholipid binding. Interestingly, heterozygotes for either 306Cys-->Gly or 316Trp-->Ser mutation have normal cardiolipin binding suggesting that beta2-glycoprotein I may circulate as a multimeric structure where wild-type subunits compensate the defective binding of the mutant ones. We investigated the effect of these mutations on quaternary structure of beta2-glycoprotein I and phospholipid binding. As previously reported, under native conditions, beta2-glycoprotein I shows an apparent molecular weight of approximately 320 kDa and it can be dissociated into subunits of lower molecular weight by boiling in 6 M urea. We show that the multimeric structure is not affected by the presence of mutations in the phospholipid-binding domain. beta2-glycoprotein I induces aggregation of anionic phospholipid vesicles suggesting again a multivalent interaction where at least two binding sites are required to bridge adjacent vesicles. beta2-glycoprotein I-induced aggregation does not cause vesicle fusion or damage as demonstrated by fluorescence resonance energy transfer (FRET) or encapsulated calcein release. In conclusion, the normal cardiolipin binding in heterozygous state for mutations at phospholipid-binding domain may be due to the multimeric structure of beta2-glycoprotein I.
Gushiken
Francisca C
FC
Division of Thrombosis Research, Department of Medicine, University of Texas Health Science Center, Houston, USA.
Le
Anhqueyn
A
Arnett
Frank C
FC
Thiagarajan
Perumal
P
D001665:Binding Sites / Q000235:genetics; D002308:Cardiolipins / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006579:Heterozygote; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes; D008970:Molecular Weight; D009154:Mutation; D010743:Phospholipids / Q000378:metabolism*; D011110:Polymorphism, Genetic; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D021122:Protein Subunits; D053482:beta 2-Glycoprotein I
D002308:Cardiolipins; D006023:Glycoproteins; D008081:Liposomes; D010743:Phospholipids; D021122:Protein Subunits; D053482:beta 2-Glycoprotein I