Preparation of spent brewer's yeast beta-glucans with a potential application as an immunostimulant for black tiger shrimp, Penaeus monodon.
Spent brewer's yeast was autolysed and the insoluble yeast cell wall fraction obtained was used as a raw material for the preparation of brewer's yeast beta-glucan (BYG). A simple alkaline extraction was applied and optimized. The BYG with significantly (P</=0.05) high carbohydrate and glucan contents of 92% and 51% (w/w), respectively and with a low protein content of 1.6% (w/w) was obtained by a single alkaline extraction using 5 volumes of 1.0 N NaOH at 90 degrees C for 1 h. In vitro, this glucan significantly (P</=0.05) enhanced phenoloxidase (PO) activity of black tiger shrimp hemolymph as compared to controls without added glucan. Also in vivo, an oral administration of 0.2% (w/w) in diets for 3 days significantly (P</=0.05) increased the PO-activity of the shrimp. The potential immunostimulating properties of a commercial and other yeast-derived products were also evaluated for comparative purposes.
Suphantharika
M
M
Department of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Road, 10400, Bangkok, Thailand. scmsp@mahidol.ac.th
Khunrae
P
P
Thanardkit
P
P
Verduyn
C
C
D000284:Administration, Oral; D000821:Animal Feed; D000822:Animal Husbandry; D000818:Animals; D017756:Aquaculture; D003247:Conservation of Natural Resources; D033561:Penaeidae / Q000276:immunology*; D011134:Polysaccharides / Q000008:administration & dosage / Q000302:isolation & purification* / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*
D011134:Polysaccharides