title: The study on culture and ultrastructure of glaucomatous trabecular cells in vitro.
abstract: OBJECTIVE
  To establish the culture system of human glaucomatous trabecular cells in vitro and study their ultrastructures.
  
  
  METHODS
  The trabecular specimens from trabeculectomy were cultured in vitro and passaged 3 times, then identified. Moreover, the glaucomatous cells were observed with electron microscope while compared with the normal ones.
  
  
  RESULTS
  Cultured human glaucomatous trabecular cells were obtained. The ultrastructure of the cells showed the decrease in vilious project, coated vesicle and lysosomal inclusion.
  
  
  CONCLUSIONS
  The establishment of human glaucomatous trabecular cells culture in vitro made the culture system more perfect. The morphologic changes might be related to the abnormal functions of human trabecular meshwork cells.
authors.affiliation: Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
authors.forename: J
authors.initials: J
authors.lastname: Ge
authors.forename: M
authors.initials: M
authors.lastname: Lin
authors.forename: J
authors.initials: J
authors.lastname: Zheng
authors.forename: Y
authors.initials: Y
authors.lastname: Zhuo
mesh_terms: D002455:Cell Division; D002478:Cells, Cultured; D003907:Dexamethasone; D005902:Glaucoma, Open-Angle / Q000139:chemically induced / Q000473:pathology*; D006801:Humans; D014129:Trabecular Meshwork / Q000166:cytology / Q000648:ultrastructure*
chemical_list: D003907:Dexamethasone