title: [Bio-derived bone transplantation with tissue engineering technique: preliminary clinical trial].
abstract: OBJECTIVE
  To sum up the clinical results of bio-derived bone transplantation in orthopedics with tissue engineering technique.
  
  
  METHODS
  From January 2000 to May 2002, 52 cases with various types of bone defect were treated with tissue engineered bone, which was constructed in vitro by allogeneous osteoblasts from periosteum (1 x 10(6)/ml) with bio-derived bone scaffold following 3 to 7 days co-culture. Among them, there were 7 cases of bone cyst, 22 cases of non-union or malunion of old fracture, 15 cases of fresh comminuted fracture of bone defect, 4 cases of spinal fracture and posterior route spinal fusion, 3 cases of bone implant of alveolar bone, 1 case of fusion of tarsotarsal joint. The total weight of tissue engineered bone was 349 g in all the cases, averaged 6.7 g in each case.
  
  
  RESULTS
  All the cases were followed up after operation, averaged in 18.5 months. The wound in all the case healed by first intention, but 1 case with second intention. Bone union was completed within 3 to 4.5 months in 50 cases, but 2 cases of delayed union. Six cases were performed analysis of CD3, CD4, CD8, ICAM-1 and VCAM-1 before and after operation, and no obvious abnormities were observed.
  
  
  CONCLUSIONS
  Bio-derived tissue engineered bone has good osteogenesis. No obvious rejection and other complications are observed in the clinical application.
authors.affiliation: Key Laboratory of Transplant Engineering and Immunology of Ministry of Health: Institute of Reparative and Reconstructive Surgeryab of Tissue ngineering, West China Hospital, Sichuan University, Chengdu, Sichuan, P. R. China 610041.
authors.forename: Zhi-ming
authors.initials: ZM
authors.lastname: Yang
authors.forename: Fu-guo
authors.initials: FG
authors.lastname: Huang
authors.forename: Ting-wu
authors.initials: TW
authors.lastname: Qin
mesh_terms: D018786:Bone Substitutes; D016025:Bone Transplantation / Q000379:methods; D002478:Cells, Cultured; D005260:Female; D005593:Fracture Fixation, Internal; D017102:Fracture Healing; D050723:Fractures, Bone / Q000601:surgery*; D005599:Fractures, Ununited / Q000601:surgery; D006801:Humans; D008297:Male; D023822:Tissue Engineering
chemical_list: D018786:Bone Substitutes