Decreased surface tension of upper airway mucosal lining liquid increases upper airway patency in anaesthetised rabbits.
The obstructive sleep apnoea syndrome (OSA) is a disorder characterised by repetitive closure and re-opening of the upper airway during sleep. Upper airway luminal patency is influenced by a number of factors including: intraluminal air pressure, upper airway dilator muscle activity, surrounding extraluminal tissue pressure, and also surface forces which can potentially act within the liquid layer lining the upper airway. The aim of the present study was to examine the role of upper airway mucosal lining liquid (UAL) surface tension (gamma) in the control of upper airway patency. Upper airway opening (PO) and closing pressures (PC) were measured in 25 adult male, supine, tracheostomised, mechanically ventilated, anaesthetised (sodium pentabarbitone), New Zealand White rabbits before (control) and after instillation of 0.5 ml of either 0.9 % saline (n = 9) or an exogenous surfactant (n = 16; Exosurf Neonatal) into the pharyngeal airway. The gamma of UAL (0.2 microl) was quantified using the 'pull-off' force technique in which gamma is measured as the force required to separate two curved silica discs bridged by the liquid sample. The gamma of UAL decreased after instillation of surfactant from 54.1 +/- 1.7 mN m-1 (control; mean +/- S.E.M.) to 49.2 +/- 2.1 mN m-1 (surfactant; P < 0.04). Compared with control, PO increased significantly (P < 0.04; paired t test, n = 9) from 6.2 +/- 0.9 to 9.6 +/- 1.2 cmH2O with saline, and decreased significantly (P < 0.05, n = 16) from 6.6 +/- 0.4 to 5.5 +/- 0.6 cmH2O with surfactant instillation. Findings tended to be similar for PC. Change in both PO and PC showed a strong positive correlation with the change in gamma of UAL (both r > 0.70, P < 0.001). In conclusion, the patency of the upper airway in rabbits is partially influenced by the gamma of UAL. These findings suggest a role for UAL surface properties in the pathophysiology of OSA.
Kirkness
Jason P
JP
Ludwig Engel Centre for Respiratory Research, Westmead Hospital and University of Sydney, New South Wales, Australia, 2145. jason_kirkness@wmi.usyd.edu.au
Christenson
Hugo K
HK
Garlick
Sarah R
SR
Parikh
Radha
R
Kairaitis
Kristina
K
Wheatley
John R
JR
Amis
Terence C
TC
D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D004338:Drug Combinations; D005233:Fatty Alcohols / Q000494:pharmacology; D008297:Male; D010614:Pharynx; D010767:Phosphorylcholine; D011092:Polyethylene Glycols / Q000494:pharmacology; D011312:Pressure; D011663:Pulmonary Surfactants / Q000494:pharmacology; D012123:Pulmonary Ventilation; D011817:Rabbits; D012121:Respiration, Artificial; D020545:Respiratory Mucosa / Q000187:drug effects / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013500:Surface Tension; D014140:Tracheotomy
D004338:Drug Combinations; D005233:Fatty Alcohols; D011663:Pulmonary Surfactants; D010767:Phosphorylcholine; D011092:Polyethylene Glycols; D012965:Sodium Chloride; C047049:dipalmitoylphosphatidylcholine, hexadecanol, tyloxapol drug combination