[Culture and identification of human vascular endothelial cells].
OBJECTIVE
To investigate the culture technigue of human vascular endothelial cells in vitro and improve the success rate of culture.


METHODS
Human vascular endothelial cells were isolated from umbilical cords by "Irrigative digestion" technique with 0.2% collagenase III and were digested by 0.0625% tripsin when the cells confluent. The cells were identified by morphologic and immunohistochemical assays.


RESULTS
The cultured human umbilical vein endothelial cells attached completely after 24 hours in vitro, reached confluence in 3-5 days with the typical appearance of "stone of pave road" and were passaged on time; Immunohistochemically these cells were stained positively by CD34 monoclonal antibody.


CONCLUSIONS
"Irrigative digestion" technique was a reliable method to obtain human umbilical vascular endothelial cells with high success rate. It can be widely used to the culture and researches of human vascular endothelial cells.
Lin
S
S
Zhongshan Ophthalmic Center, Sun Yet-sen University of Medical Sciences, Guangzhou, 510060.
Tang
S
S
Hu
J
J
Zheng
J
J
Zheng
H
H
Luo
Y
Y
D000911:Antibodies, Monoclonal / Q000378:metabolism; D018952:Antigens, CD34 / Q000276:immunology; D018929:Cell Culture Techniques / Q000379:methods; D002469:Cell Separation; D002478:Cells, Cultured; D017364:Collagenases; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D053509:Matrix Metalloproteinase 13; D014471:Umbilical Veins / Q000166:cytology
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D017364:Collagenases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13