[SDS-PAGE and EITB analysis of the protein components of different isolates of Schistosoma japonicum in China and Japan].
OBJECTIVE
To make a supplementary observation on the protein components prepared from adult S. japonicum of 4 different isolates from Zhejiang, Jiangxi, Taiwan Provinces of China and Japan in origin and to observe antigen reactivity of the above-mentioned isolates together with adult worms from Anhwi, Hubei, Sichuan and Yunnan isolates against heterologous anti-Oncomelania h. hupensis (collected from Guangxi, China and Japan) sera by EITB.


METHODS
SDS-PAGE and EITB.


CONCLUSIONS
SDS-PAGE showed that by Coomassie blue staining, male S. japonicum from Jiangxi, Zhejiang, Taiwan Provinces and Japan isolales revealed 7-17 bands while female worms revealed 1-6 bands. The protein patterns of Taiwan and Zhejiang male worm were similar, but slight difference could be seen above 81 kDa. By silver staining, male worms of the 4 isolates revealed 10-23 bands while female worms revealed 1-19 bands. Male worms from Japan not only showed less bands but also differed in their pattern as compared to those of other Chinese isolates. Results of EITB revealed that each of the 4 isolates had slightly different pattern but all of the tested isolates had common antigens with their heterologous snail hosts i.e., Oncomelania h. hupensis from Guangxi, China and Japan.
Qiu
L
L
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Liu
S
S
Xue
H
H
Zhang
Y
Y
Li
H
H
Hu
Y
Y
He
Y
Y
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D002681:China; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015801:Helminth Proteins / Q000032:analysis*; D015151:Immunoblotting; D007564:Japan; D008297:Male; D012549:Schistosoma japonicum / Q000737:chemistry* / Q000145:classification / Q000276:immunology; D013045:Species Specificity
D000947:Antigens, Helminth; D015801:Helminth Proteins