[Cloning and sequencing of cytochrome c oxidase II(COII) gene of three species of mosquitoes].
OBJECTIVE
To determine and compare the complete sequence of COII gene from Anopheles sinensis, Aedes albopictus and Culex quinquefasciatus and to identify the molecular homology among these mosquitoes.


METHODS
The recombinant plasmids including COII gene from the three species of mosquitoes were obtained by the T-A cloning technique, and the COII genes were sequenced and analyzed.


RESULTS
The homology of the COII gene nucleotide sequence and the deduced amino acid sequence of COII among the three species of mosquitoes varied from 84.1% to 87.9% and from 85.1% to 89.5%, respectively. The C + G content of COII gene was 23.2%-24.9%, the frequency of transversion higher than that of transition.


CONCLUSIONS
Aedes albopictus and Culex quinquefasciatus are closely related species at their COII molecular level according to COII gene.
Huang
Z H
ZH
College of Life Sciences, Zhejiang University, Hangzhou 310012.
Wang
J F
JF
D000330:Aedes / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003465:Culex / Q000201:enzymology / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
C117108:cytochrome C oxidase subunit II; D003576:Electron Transport Complex IV