[The Antisense Oligodeoxynucleotides of bcl-2 Oncogene Enhances the Sensitivity of K562 Leukemic Cells to As(2)O(3)]
The biological reaction, changes of cellular DNA and bcl-2 protein content of K562 cells to bcl-2 ASODN or As(2)O(3) alone or bcl-2 ASODN associated with As(2)O(3) were investigated by cellular microculture, immunological tissue-chemistry method, and flow cytometry. The results showed that there is much more induce-apoptotic effect of As(2)O(3) on K562 cells while it combined with bcl-2 ASODN (ASODN 10.0 micro mol/L + As(2)O(3) 5.0 micro mol/L) than bcl-2 ASODN or As(2)O(3) alone (ASODN 10.0 micro mol/L or As(2)O(3) 5.0 micro mol/L), (P < 0.01), and so does inhibitory effect of bcl-2 protein expression by flow cytometry (P < 0.01). In conclusions, the bcl-2 ASODN can enhance the drug-sensetivity of K562 leukemic cells to As(2)O(3).
Sheng
Wei-Li
WL
Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China; Research Department of Fever, Medical College of Jinan University, Guangzhou 510632, China.
Li
Hong
H
Zhang
Yuan
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