title: [The expression of humanized Fab fragment of the anti-HBsAg antibody in methylotropic yeast Pichia pastoris].
abstract: Using of two-step integrating technology, transducted the H and L chain gene of humanized Fab fragment of anti-HB-sAg antibody into the genome of methylotropic yeast P. pastoris. Constructed a engineering yeast to produce humanized Fab fragment of the anti-HBsAg antibody. The Fab fragment was efficiently secreted into the medium at a concentration of 50-80 mg/L. The Fab fragment was purified from culturing supernatant of the recombinant yeas by affinity chromatography. The ELISA analysis showed the high affinity of the expressed humanized Fab fragment to the HBsAg.
authors.affiliation: State Key Laboratory for Biological Control, Biopharmaceutical Center in Zhongshan University, Guangzhou 510275, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Deng
authors.forename: Kuan-Yuan
authors.initials: KY
authors.lastname: Su
authors.forename: Xun-Zhang
authors.initials: XZ
authors.lastname: Wang
authors.forename: Qing-Xin
authors.initials: QX
authors.lastname: Long
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.forename: Zhou-Yao
authors.initials: ZY
authors.lastname: Yu
mesh_terms: D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D006510:Hepatitis B Antibodies / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D010843:Pichia / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D007140:Immunoglobulin Fab Fragments; D011994:Recombinant Proteins