[Calcium-regulated growth and differentiation of the mouse epidermal keratinocytes].
The effect of the calcium ion (Ca2+) on the growth and differentiation of the mouse epidermal keratinocytes cultured in serum-free medium was investigated. It was found that the optimal level of calcium ion in the medium was about 0.2 mmol/L. Under such a culture condition the colony forming efficiency, attachment percentage, percentage of the cells with cornified envelops, and percentage of the senesced cells were measured to be about 10.8%, 30.8%, 5.1%, and 26.8%, respectively. However, the Ca2+ concentrations in the medium above 0.6 mmol/L resulted in significant differentiation and senescence of the keratinocytes, which was found to be harmful for keratinocyte growth and expansion in vitro.
Yu
Hai-Qiang
HQ
State Key Laboratory of Bioreactor Engineering, ECUST, Shanghai 200237, China.
Zhou
Yan
Y
Hua
Ping
P
Tan
Wen-Song
WS
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects; D000078404:Epidermal Cells; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects*; D051379:Mice
D002118:Calcium