[Gene expression of insulin-like growth factors in human villous trophoblast cells of early pregnancy in vitro].
OBJECTIVE
To investigate the gene expression of insulin-like growth factors(IGFs) in human villous trophoblast cells of early pregnancy in vitro and their action.


METHODS
Semiquantitative reverse transcriptase polymerase chain reaction(RT-PCR), with beta-ACTIN as internal standard, was applied to determine the expression of IGFs messenger RNA in human villous trophoblast cells of early pregnancy in vitro.


RESULTS
The expression of IGF-II mRNA, IGF-I R mRNA and IGFBP-3 mRNA was detectable in human villous trophoblast cells of early pregnancy in vitro.


CONCLUSIONS
IGFs appear to play important regulation roles in early invasion, proliferation and differentiation of cytotrophoblast, and in the formation of placenta and the development of embryo via autocrine and/or paracrine way.
Li
Lei
L
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Li
Shangwei
S
Cao
Zeyi
Z
Peng
Zhilan
Z
Han
Ziyan
Z
D000328:Adult; D002455:Cell Division; D002478:Cells, Cultured; D002824:Chorionic Villi / Q000378:metabolism*; D005260:Female; D006133:Growth Substances / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014327:Trophoblasts / Q000378:metabolism*
D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C065700:myotrophin