[Analysis on metabolic flux shift during guanosine fermentation].
Taking the typical metabolic control product-guanosine as an example, the method of metabolic flux shift investigation based on process multi-levels parameter correlation analysis was established. The metabolic pathway, multi-parameter correlation, accumulation of amino acid and organic acid during guanosine fermentation process were integratively analyzed. The metabolic flux shift from HMP to EMP was ascertained, which was assumed to be caused by the accumulation of ammonium ion. The subsequent optimization based on controlling flux distribution between EMP and HMP did improve the yield by 35% when the metabolic flux shift was prevented.
Cai
Xian-Peng
XP
State Key Laboratory of Bioreactor Engineering, ECUST, National Engineering Research Center for Biotechnology, Shanghai 200237, China.
Chen
Shuang-Xi
SX
Chu
Ju
J
Zhuang
Ying-Ping
YP
Zhang
Si-Liang
SL
D000596:Amino Acids / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D005285:Fermentation; D006151:Guanosine / Q000378:metabolism*
D000596:Amino Acids; D006151:Guanosine; D000641:Ammonia