Repeated DNA sequences in the microbat species Miniopterus schreibersi (Vespertilionidae; Chiroptera).
Repetitive DNA sequences represent a substantial component of eukaryotic genomes. These sequences have been described and characterized in many mammalian species. However, little information about repetitive DNA sequences is available in bat species. Here we describe an EcoRI family of repetitive DNA sequences present in the species Miniopterus schreibersi. These repetitive sequences are 57.85%, A-T rich, organized in tandem, and with a monomer unit length of 904 bp. Methylation analysis using the isoesquizomer pair MspI and HpaII indicates that the cytosines present in the sequences CCGG are partially methylated. Furthermore, Southern blot analysis demonstrated that these DNA sequences are absent in the genomes of four related microbat species and suggest that it could be specific to the M. schreibersi genome.
Barragán
M J L
MJ
Departamento de Biologia Experimental, Facultad de Ciencias Experimentales y de la Salud, Universidad de Jaén, Jaén, Spain.
Martínez
S
S
Marchal
J A
JA
Bullejos
M
M
Díaz de la Guardia
R
R
Sánchez
A
A
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002685:Chiroptera / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D004247:DNA