title: Analysis of sequential immunoglobulin E-binding epitope of Japanese cedar pollen allergen (Cry j 2) in humans, monkeys and mice.
abstract: BACKGROUND
  Japanese cedar (Cryptomeria japonica; CJ) pollinosis has been reported to occur naturally in Japanese monkeys (Macaca fuscata) as well as in humans. Most human patients and monkeys with pollinosis have specific IgE for Cry j 2, a major allergen of CJ pollen.
  
  
  OBJECTIVE
  The main purpose of this study was to identify IgE B cell epitopes of Cry j 2 using a synthetic peptide in humans, monkeys and mice.
  
  
  METHODS
  We synthesized 38 overlapping peptides that span the entire length of Cry j 2. We examined the B cell epitopes of Cry j 2 that are recognized by IgE in the sera of human patients and monkeys with pollinosis and immunized mice using synthetic peptides of Cry j 2. We also examined the reaction of Cry j 2-specific mouse monoclonal IgG antibodies to the peptides. Furthermore, we conducted a histamine release assay with leucocytes from a pollinosis patient using human serum albumin (HSA) conjugated with the peptides as a B cell epitope.
  
  
  RESULTS
  We found that 16 of the 20 pollinosis patients who had specific IgE to Cry j 2 also exhibited IgE reaction with some Cry j 2 peptides. Of these 16 patients, 10 exhibited IgE reaction with Cry j 2 peptide no. 13 (121GQCKWVNGREICNDRDRPTA140). Five of the seven monkeys with CJ pollinosis exhibited a reaction with peptide no. 13. Furthermore, IgE in mice immunized with Cry j 2 and two mouse monoclonal IgG antibodies reacted with peptide no. 13. Peptide no. 13-conjugated HSA showed the release of histamine from basophils. Furthermore, to determine the minimum epitope in peptide no. 13, we conducted an enzyme-linked immunosorbent assay inhibition test. The core of the epitope in humans, monkeys and mice was 124KWVNGREI131.
  
  
  CONCLUSIONS
  We found that 124KWVNGREI131 is an important B cell epitope recognized by IgE in humans, monkeys and mice.
authors.affiliation: Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tamura
authors.forename: J
authors.initials: J
authors.lastname: Kawaguchi
authors.forename: N
authors.initials: N
authors.lastname: Serizawa
authors.forename: K
authors.initials: K
authors.lastname: Hirahara
authors.forename: A
authors.initials: A
authors.lastname: Shiraishi
authors.forename: H
authors.initials: H
authors.lastname: Nigi
authors.forename: Y
authors.initials: Y
authors.lastname: Taniguchi
authors.forename: M
authors.initials: M
authors.lastname: Toda
authors.forename: S
authors.initials: S
authors.lastname: Inouye
authors.forename: T
authors.initials: T
authors.lastname: Takemori
authors.forename: M
authors.initials: M
authors.lastname: Sakaguchi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000918:Antibody Specificity; D018985:Epitopes, B-Lymphocyte / Q000378:metabolism*; D005260:Female; D006636:Histamine Release / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism*; D008251:Macaca / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D010446:Peptide Fragments / Q000276:immunology; D010940:Plant Proteins / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology*; D012882:Skin Tests / Q000379:methods; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; C065291:Cry j II protein, Cryptomeria japonica; D018985:Epitopes, B-Lymphocyte; D010446:Peptide Fragments; D010940:Plant Proteins; D007073:Immunoglobulin E