title: A sense of closeness: protein detection by proximity ligation.
abstract: Highly specific and sensitive procedures will be required to evaluate proteomes. Proximity ligation is a recently introduced mechanism for protein analysis. In this technique, the convergence of sets of protein-binding reagents on individual target molecules juxtaposes attached nucleic acid sequences. Through a ligation reaction a DNA reporter sequence is created, which can be amplified. The procedure thus encodes detected proteins as specific nucleic acid sequences in what may be viewed as a reverse translation reaction.
authors.affiliation: The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, Se-751 85 Uppsala, Sweden.
authors.forename: Mats
authors.initials: M
authors.lastname: Gullberg
authors.forename: Simon
authors.initials: S
authors.lastname: Fredriksson
authors.forename: Michael
authors.initials: M
authors.lastname: Taussig
authors.forename: Jonas
authors.initials: J
authors.lastname: Jarvius
authors.forename: Sigrun
authors.initials: S
authors.lastname: Gustafsdottir
authors.forename: Ulf
authors.initials: U
authors.lastname: Landegren
mesh_terms: D001483:Base Sequence; D015342:DNA Probes; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D046911:Macromolecular Substances; D015336:Molecular Probe Techniques; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D009841:Oligonucleotides / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D020539:Sequence Analysis, Protein / Q000379:methods*
chemical_list: D015342:DNA Probes; D046911:Macromolecular Substances; D009841:Oligonucleotides; D011506:Proteins