[Determination of six effective components in Rheum by cyclodextrin modified micellar electrokinetic chromatography].
OBJECTIVE
To determine six effective components (aloe-emodin, rhein, emodin, rhaponticin, physcion and chrysophanol) in Rheum.


METHODS
Using buffer solution containing 20 mmol.L-1 borax, 20 mmol.L-1 sodium deoxycholate (SDC), 20 mmol.L-1 sodium taurocholate (STC), 15 mmol.L-1 beta-cyclodextrin (beta-CD) and O-phthalic acid as the internal standard, the six components were determined by cyclodextrin modified micellar electrokinetic chromatography.


RESULTS
In less than 25 min, aloe-emodin, rhein, emodin, rhaponticin, physion and chrysophanol were separated. The separation conditions were optimized by adjusting buffer pH, concentrations of SDC, STC and beta-CD. The linearity ranges of aloe-emodin, rhein, emodin, rhaponticin, physcion and chrysophanol were 4-34, 5-40, 4-60, 5-80, 6-90 and 5-85 micrograms.mL-1 respectively. Relative standard deviation (RSD) of the method was less than 2.2%. The recoveries of aloe-emodin, rhein, emodin, rhaponticin, physcion and chrysophanol were 100.0%, 98.3%, 100.4%, 94.6%. 95.2% and 93.8% respectively. Raw Rheum, Mongolian Rheum and Rheum tanguticum samples were analyzed.


CONCLUSIONS
This method can be an effective one for identification of Rhubarb.
Shang
Xiao-yu
XY
Institute of Applied Chemistry, Graduate School of the University of Science and Technology of China, Beijing 100039, China. xiaoyushang@eyou.com
Yuan
Zhuo-bin
ZB
D000880:Anthraquinones / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods; D003505:Cyclodextrins / Q000737:chemistry; D004642:Emodin / Q000031:analogs & derivatives* / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D012250:Rheum / Q000737:chemistry*; D013267:Stilbenes / Q000032:analysis
D000880:Anthraquinones; D003505:Cyclodextrins; D013267:Stilbenes; C059635:aloe emodin anthrone; C518327:aloe emodin; C023538:rhapontin; D004642:Emodin; C020491:rhein