[Anti-hemolytic and anti-membranous lipid peroxidation effects of daphnetin].
OBJECTIVE
To investigate the anti-hemolytic and anti-membranous lipid peroxidation effects of daphnetin.


METHODS
The inhibition rates of daphnetin on AQD-induced hemolysis and erythrocytic membranous lipid peroxidation were determined by routine in vitro assay for detecting hemolytic toxicity and by HPLC for detecting membranous lipid peroxidation products.


RESULTS
Daphnetin at a range of 10-80 mumol/L inhibited dose-dependently AQD-induced hemolysis and lipid peroxidation by 33.0%-69.2% and 11.9%-58.2%, respectively.


CONCLUSIONS
Daphnetin has anti-hemolytic and anti-erythrocytic membranous lipid peroxidation effects.
Ni
Y
Y
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Xu
Y
Y
Wang
M
M
Liu
Y
Y
D000634:Aminoquinolines / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D011817:Rabbits; D014468:Umbelliferones / Q000494:pharmacology*
D000634:Aminoquinolines; D000975:Antioxidants; D014468:Umbelliferones; C000981:5,6-dihydroxy-8-aminoquinoline; C039952:daphnetin