[Effect of IL-6 on the multiplication of Toxoplasma gondii].
OBJECTIVE
To observe the effect of IL-6 on the multiplication of Toxoplasma gondii.


METHODS
Peritoneal macrophages from C57BL/6 mouse were incubated with 3H-uracil-labelled T. gondii in vitro.


RESULTS
Pretreatment (but not post-treatment) of Mphi with IL-6 enhanced T. gondii multiplication in a dose-dependent manner. Pretreatment with IFN gamma resulted in active killing of parasites whereas the addition of IL-6 resulted in a partial reversal of IFN gamma-mediated toxoplasmacidal activity. Combining TNF alpha with IL-6 and IFN gamma pretreatment resulted in restoration of toxoplasmacidal activity. Addition of polyclonal anti-TNF alpha antibodies to IL-6 and IFN gamma pretreatment resulted in enhancement in the IL-6-mediated impairment of IFN gamma function.


CONCLUSIONS
IL-6 could enhance intraperitoneal multiplication of T. gondii and reverse IFN gamma-mediated toxoplasmacidal activity.
Fang
Y
Y
Central Lab., First Teaching Hospital, Bethune University of Medical Sciences, Changchun 130021.
Tan
Y
Y
Zhang
Y
Y
Li
J
J
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000469:parasitology*; D051379:Mice; D008810:Mice, Inbred C57BL; D014122:Toxoplasma / Q000187:drug effects / Q000254:growth & development*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma