[The experimental and clinical study of inhibitory effects of Re-Du-Qing on human cytomegalovirus (HCMV)].
OBJECTIVE
To study the theurapeutic effect of Chinese medicine Re-Du-Qing on HCMV.


METHODS
The expression of the HCMV late-mRNA in infected cells was measured by quantitative RT-PCR and the development of cytopathic effect (CPE) caused by HCMV was observed before and after the treatment of Chinese medicine "Re-Du-Qing". On the basis of its anti-HCMV activity study in vitro, Re-Du-Qing was applied to the child-bearing aged women and to pregnant women who were HCMV-IgM positive which indicated the active HCMV infection, meanwhile the serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-2 (IL-2) were measured before and after the treatment by the ELISA method.


RESULTS
The results showed that the Re-Du-Qing had strong inhibition effect both on HCMV-mRNA and CPE. The serum level of the TNF-alpha increased and IL-2 decreased significantly in HCMV-IgM positive cases when compared with normal women. After being treated with Re-Du-Qing, the serum TNF-alpha and IL-2 in patients returned to normal level, and 72.9% of the HCMV-IgM positive cases turned negative which was significantly higher than that (13.6%) in control group.


CONCLUSIONS
It was suggested that serum TNF-alpha level is closely related to the activity of HCMV which has harmful effect on immune system. Re-Du-Qing can enhance immunity of the body and has an obvious inhibition on HCMV in Vitro and Vivo, and can thus serve as safe and effective medicine for treatment of active HCMV infection, especially for the pregnant women.
Jiang
H
H
Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical University, Wuhan 430030.
Wen
L
L
Ling
X
X
D000328:Adult; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000188:drug therapy / Q000821:virology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D008168:Lung / Q000166:cytology; D011247:Pregnancy; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000914:Antibodies, Viral; D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D007075:Immunoglobulin M; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha