Different cellular fatty acid pattern behaviours of two strains of Listeria monocytogenes Scott A and CNL 895807 under different temperature and salinity conditions.
Cells of two strains of Listeria monocytogenes CNL 895807 and Scott A were grown to late exponential phase at different growth temperatures (37, 20 and 4 degrees C) with or without NaCl (7%), and their fatty acid compositions were analysed. The results showed that low thermal adaptation response of L. monocytogenes CNL was different than that of the Scott A strain, and it was based on both an increase of anteiso-branched-chain fatty acids and a significant decrease of straight-chain fatty acids. However, the main modifications observed in the Scott A strain when grown at a low temperature were a decrease of the proportion of ai17:0 and an increase of ai15:0. In hyperosmotic medium and over the entire temperature range (4 degrees C, 20 degrees C and 37 degrees C) the two L. monocytogenes strains showed a cellular fatty acid profile dominated by ai15:0. In addition, a decrease of the two major straight-chain fatty acids (14:0 and 16:0) was observed in the CNL strain. These results demonstrated that the CNL strain showed different behaviours of low thermal and salt adaptation to maintain membrane fluidity, which are based both on an increase of anteiso-branched-chain fatty acids, and a significant decrease of straight-chain fatty acids.
Chihib
Nour-Eddine
NE
Université des Sciences et Technologies de Lille, Laboratoire de Microbiologie, Bâtiment SN2, Cité Scientifique, 59655 Villeneuve d'Ascq Cedex, France. nour-eddine.chohib@univ-lille.l.fr
Ribeiro da Silva
Margarida
M
Delattre
Gilles
G
Laroche
Michel
M
Federighi
Michel
M
D000222:Adaptation, Physiological; D003470:Culture Media / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D013696:Temperature
D003470:Culture Media; D005227:Fatty Acids; D012965:Sodium Chloride