Evaluation of indices of skeletal muscle contraction in areas of referred hyperalgesia from an artificial ureteric stone in rats.
This study examined indices of skeletal muscle contraction in a rat model of referred muscle hyperalgesia from artificial ureteric calculosis [left oblique muscle (OE) for ipsilateral stone]. In specimens from the left versus right OE of stone-implanted female rats, a significant increase was found in membrane fluidity (P<0.01) and Ca(2+)-ATPase activity (P<0.0001) and a significant decrease in 3H-ryanodine binding (P<0.0001) and in I band length/sarcomere length ratio (contraction index) (P<0.01). The increase in Ca(2+)-ATPase activity was directly and significantly related to the number of rats' ureteral 'crises' (P<0.02). The results indicate a state of contraction in the hyperalgesic muscle, whose extent correlates to the algogenic activity of the ureteral stone.
Giamberardino
Maria Adele
MA
Department of Medicine and Science of Aging, 'G D'Annunzio' University of, Chieti, Italy. mag@unich.it
Affaitati
Giannapia
G
Lerza
Rosanna
R
Fanò
Giorgio
G
Fulle
Stefania
S
Belia
Silvia
S
Lapenna
Domenico
D
Vecchiet
Leonardo
L
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002462:Cell Membrane / Q000502:physiology; D005260:Female; D006930:Hyperalgesia / Q000503:physiopathology*; D008560:Membrane Fluidity / Q000502:physiology*; D008854:Microscopy, Electron; D023421:Models, Animal; D009119:Muscle Contraction / Q000502:physiology; D018482:Muscle, Skeletal / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012433:Ryanodine / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D014514:Ureteral Calculi / Q000473:pathology / Q000503:physiopathology*
D012433:Ryanodine; D017301:Ca(2+) Mg(2+)-ATPase