title: [A new method for detecting serum beta-lipoprotein electrophoretogram].
abstract: OBJECTIVE
  To establish a new method for isolating serum beta-lipoprotein (betaLp) subfractions using second double gradient polyacrylamide gel electrophoresis (s-DGPAGE).
  
  
  METHODS
  After the betaLp subfractions were isolated by mechanisms of molecular sieving and isoelectric focusing due to density and pH grade in the non-linear density gradient polyacrylamide gel, the gels were acquired with a gel imaging system, the electrophoretograms were quantitatively analyzed with optical density scanner. The isolation of serum betaLp was completed through first DGPAGE.
  
  
  RESULTS
  s-DGPAGE separated the serum betaLp into four subfractions, designated respectively as betaLp1, betaLp2, betaLp3 and betaLp4, and their amounts in the betaLp and in the whole lipoproteins could be quantified.
  
  
  CONCLUSIONS
  s-DGPAGE is a simple and practical method to show the heterogeneity of serum betaLp and the quantitative variation of its subfractions.
authors.affiliation: Electrocardiology Research Center, Department of Basic Medicine, First Military Medical University, Guangzhou 510515, China. SLPG@fimmu.edu.cn
authors.forename: Yun-qin
authors.initials: YQ
authors.lastname: Chen
authors.forename: Jia
authors.initials: J
authors.lastname: Yin
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Bing-sheng
authors.initials: BS
authors.lastname: Yin
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D008077:Lipoproteins, LDL / Q000032:analysis / Q000097:blood*; D015203:Reproducibility of Results
chemical_list: D008077:Lipoproteins, LDL