title: Heat inactivation of mammalian cell cultures for biowaste kill system design.
abstract: A biowaste kill system was implemented to treat biological waste generated from a clinical manufacturing and R&D antibody facility. To confirm that design parameters of this continuous decontamination system are sufficient to inactivate mammalian cell culture waste, bench-scale experiments were conducted. The biowaste kill system heat inactivates mammalian cell cultures before they are piped to a neutralization tank and subsequently released to the sewage system. Heat inactivation of cells is accomplished by exposing cells to 80 degrees C for 1 min. Small-scale heat inactivation studies were performed on CHO, 293-HEK, and hybridoma cells. Cells at 1 x 10(6) cells/mL or 1 x 10(7) cells/mL were exposed to 37, 60, 70, or 80 degrees C for 0, 30, 60, and 120 s. Viability based on trypan blue exclusion method and ability to proliferate was assessed after exposure to heat. Data suggest that exposure of cells to 80 degrees C for 60 s is sufficient to inactivate these cultures before they are released to the sewage system.
authors.affiliation: Abgenix, Inc., Department of Process Sciences, 6701 Kaiser Dr., MS26, Fremont, California 94555, USA. niki.gregoriades@abgenix.com
authors.forename: Niki
authors.initials: N
authors.lastname: Gregoriades
authors.forename: Melissa
authors.initials: M
authors.lastname: Luzardo
authors.forename: Beatrice
authors.initials: B
authors.lastname: Lucquet
authors.forename: Thomas
authors.initials: T
authors.lastname: Ryll
mesh_terms: D000818:Animals; D019149:Bioreactors; D016466:CHO Cells / Q000502:physiology / Q000528:radiation effects; D002470:Cell Survival / Q000502:physiology / Q000528:radiation effects; D006224:Cricetinae; D003666:Decontamination / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D006358:Hot Temperature; D006801:Humans; D006825:Hybridomas / Q000502:physiology / Q000528:radiation effects; D007220:Industrial Waste / Q000517:prevention & control; D007668:Kidney / Q000196:embryology / Q000502:physiology / Q000528:radiation effects; D008322:Mammals; D017744:Medical Waste Disposal / Q000295:instrumentation* / Q000379:methods*; D010865:Pilot Projects; D012680:Sensitivity and Specificity; D013242:Sterilization / Q000295:instrumentation* / Q000379:methods*; D013696:Temperature
chemical_list: D007220:Industrial Waste; D017744:Medical Waste Disposal