[Structure and regulative function of the 5' end flanking sequence of gene CCR5].
OBJECTIVE
To construct the pCAT reporter genes containing the 5'-end flanking of CC chemokine receptor 5(CCR5) gene in different sequence lengths and identify the sequence, which regulates the gene expression of CCR5 by the CAT analysis system.


METHODS
The target sequences were amplified by pyrobest DNA polymerase, and were inserted into the upstream of CAT gene located in the pCAT enhancer vector by the directional clone technique respectively; the regulative sequence was identified by analyzing the CAT activities of reconstructed plasmid in Hela cells.


RESULTS
The region, containing 486 bp upstreaming from exon 1, stimulated the reporter gene activity which was about 3 times that of the pCAT enhancer vector in transfected cells.


CONCLUSIONS
There is an up-regulative element of gene transcription in the region of -1(-)-486 bp in CCR5 gene upstream.
Sha
Xin-ping
XP
Institute of Infectious Disease, Xiangya Hospital, Central South University, Changsha 410008, China.
Hu
Guo-ling
GL
Xie
Yu-tao
YT
D024506:5' Flanking Region / Q000235:genetics*; D003001:Cloning, Molecular; D004742:Enhancer Elements, Genetic / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D008297:Male; D011401:Promoter Regions, Genetic / Q000235:genetics; D019713:Receptors, CCR5 / Q000235:genetics* / Q000502:physiology
D019713:Receptors, CCR5