[Cloning of Cysticercus cellulosae AgB cDNA coding region].
OBJECTIVE
To amplify and clone Cysticercus cellulosae AgB cDNA coding region.


METHODS
The AgB cDNA was amplified by RT-PCR technique from the total RNA of Cysticercus cellulosae. It was cloned into the vector pUC118 and sequenced.


RESULTS
The PCR amplified product was a single band of 2.6 kb in size. The sequences of AgB cDNA coding region has 99.8% homology with that of Australian Cysticercus cellulosae, and their amino acid sequences have 99.3% homology.


CONCLUSIONS
Cysticercus cellulosae AgB cDNA coding region has been cloned successfully.
Wang
Q M
QM
Department of Medical Genetic, Second Military Medical University, Shanghai 200433.
Dai
J X
JX
Zhang
P W
PW
Sun
S H
SH
D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003552:Cysticercus / Q000276:immunology*; D018076:DNA, Complementary / Q000235:genetics; D005784:Gene Amplification; D008969:Molecular Sequence Data; D012313:RNA / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D014335:Tropomyosin / Q000235:genetics*
D000947:Antigens, Helminth; D018076:DNA, Complementary; D014335:Tropomyosin; D012313:RNA