Advances in quantitative proteomics via stable isotope tagging and mass spectrometry.
The high-throughput identification and accurate quantification of proteins are essential components of proteomic strategies for studying cellular functions and processes. Techniques that are largely based on stable isotope protein or peptide labeling and automated tandem mass spectrometry are increasingly being applied in quantitative proteomic studies. Over the past year, significant progress has been made toward improving and diversifying these technologies with respect to the methods for stable isotope labeling, process automation and data processing and analysis. Advances in stable isotope protein labeling and recent biological studies that used stable isotope based quantitative proteomics techniques are reviewed.
Tao
W Andy
WA
Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA.
Aebersold
Ruedi
R
D000345:Affinity Labels / Q000138:chemical synthesis* / Q000737:chemistry; D000596:Amino Acids; D020869:Gene Expression Profiling / Q000379:methods; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D010103:Oxygen Isotopes; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D000345:Affinity Labels; D000596:Amino Acids; D007202:Indicators and Reagents; D010103:Oxygen Isotopes; D011506:Proteins; D020543:Proteome; D011994:Recombinant Proteins