Association of hepatitis virus infection, alcohol consumption and plasma vitamin A levels with urinary 8-hydroxydeoxyguanosine in chemical workers.
Urinary 8-hydroxydeoxyguanosine (8-OHdG) DNA adduct has been used as a biomarker in epidemiological studies. However, the determinants for urinary 8-OHdG have not been clearly identified. We tested urinary 8-OHdG levels in 205 male workers who had been exposed to vinyl chloride monomer (VCM). Epidemiological information was obtained by an interviewer-administered questionnaire. Hepatitis B surface antigen (HBsAg) and anti-hepatitis C antibody (anti-HCV) were also determined by immunoassay. Plasma antioxidants including Vitamins A and E, alpha- and beta-carotenes were assayed by high performance liquid chromatography. Median of urinary 8-OHdG level was 9.8 ng/mg creatinine (range, 1.4-60.1). Multiple linear regression analysis showed that alcohol drinkers had higher urinary 8-OHdG than those who did not, but there was no dose-response between the amount of alcohol consumption and urinary 8-OHdG. Workers with positive HBsAg, anti-HCV and elevated plasma Vitamin A level were independently associated with higher levels of urinary 8-OHdG, whereas age, smoking, body mass index, plasma alpha- and beta-carotenes, Vitamin E levels, or VCM exposure did not show such an association. The results suggest that active inflammation of hepatitis B and C, alcohol consumption and higher Vitamin A level can induce oxidative stress. Thus, we conclude that potential determinants need to be considered in epidemiological studies when urinary 8-OHdG is used as a biomarker.
Wong
Ruey-Hong
RH
Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University No. 1 Ren-Ai Rd., Sec. 1, 10018, Taipei, Taiwan.
Yeh
Ching-Ying
CY
Hsueh
Yu-Mei
YM
Wang
Jung-Der
JD
Lei
Yu-Chen
YC
Cheng
Tsun-Jen
TJ
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000428:Alcohol Drinking / Q000009:adverse effects / Q000097:blood / Q000652:urine; D002273:Carcinogens / Q000009:adverse effects / Q000494:pharmacology; D003849:Deoxyguanosine / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000652:urine*; D006525:Hepatitis, Viral, Human / Q000097:blood / Q000209:etiology / Q000652:urine; D006801:Humans; D008297:Male; D016273:Occupational Exposure / Q000009:adverse effects; D014752:Vinyl Chloride / Q000009:adverse effects / Q000494:pharmacology; D014801:Vitamin A / Q000097:blood*
D002273:Carcinogens; D014801:Vitamin A; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D014752:Vinyl Chloride