title: Determination of disk diffusion and MIC quality control parameters for AZD2563, a novel long-acting oxazolidinone.
abstract: AZD2563 is a new, long-acting oxazolidinone that possesses potent antimicrobial activity against Gram-positive organisms. This report summarizes the results of broth microdilution MIC and disk diffusion quality control (QC) investigations for AZD2563. All tests or methods (M2-A7 and M7-A5) and QC study designs (M23-A2) were those published by the National Committee for Clinical Laboratory Standards. QC strains tested against AZD2563 included: Staphylococcus aureus ATCC 25923 and ATCC 29213, Enterococcus faecalis ATCC 29212 and Streptococcus pneumoniae ATCC 49619. In the eight-center QC trial, 320 and 480 results were generated for MIC and disk diffusion tests, respectively. The selected 7-mm ranges for the two disk diffusion QC organisms contained > or = 98.5% of reported results. Proposed 3 or 4 log(2) dilution MIC ranges contained all reported participant results. AZD2563 disk diffusion zone diameters were generally 2-3 mm smaller than the corresponding linezolid zone diameters. In conclusion, QC ranges for AZD2563 MIC and disk diffusion methods were established and appear acceptable in preparation for the clinical trials use.
authors.affiliation: The JONES Group/JMI Laboratories, North Liberty, Iowa, USA.
authors.forename: Tamara R
authors.initials: TR
authors.lastname: Anderegg
authors.forename: Ronald N
authors.initials: RN
authors.lastname: Jones
mesh_terms: D004058:Diffusion; D004352:Drug Resistance, Microbial; D013293:Enterococcus faecalis / Q000187:drug effects / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D023303:Oxazolidinones / Q000494:pharmacology*; D011786:Quality Control; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000302:isolation & purification
chemical_list: D023303:Oxazolidinones; C453362:posizolid