title: Isolation of H3N2 swine influenza virus in South Korea.
abstract: Swine influenza is a significant respiratory disease causing occasional reproductive problems in naïve swine herds. Although different subtypes of swine influenza virus (SIV) have been implicated in clinical outbreaks of swine influenza in Asian countries, no virus isolation has been made to identify SIV of subtypes other than the H1N1 subtype in the Korean swine population. In December 1998, an outbreak of acute respiratory disease was identified in a commercial swine farm located in the Kyunggi province of South Korea. A causative agent, which agglutinated rooster red blood cells, was detected from the lungs of 3 piglets from the index herd and was determined to be type A influenza virus using a commercial influenza virus typing kit. Hemagglutination activity (HA) of the isolates was completely inhibited by a swine antiserum against a recent US H3N2 SIV isolate (A/Sw/IA/41305/1998) but not by H1N1 swine antiserum (A/Sw/IA/1979). Reverse transcription-polymerase chain reaction (RT-PCR) revealed all 3 isolates were H3 SIV subtypes. Sequence analysis of hemagglutinin gene PCR products supported the belief that the Korean H3 SIV isolates were genetically similar to the known mammalian H3 influenza viruses. This is the first report on a clinical outbreak of swine influenza caused by the H3N2 virus in Korea.
authors.affiliation: Department of Veterinary Medicine Virology Laboratory, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, South Korea.
authors.forename: Dae S
authors.initials: DS
authors.lastname: Song
authors.forename: Jae Y
authors.initials: JY
authors.lastname: Lee
authors.forename: Jin S
authors.initials: JS
authors.lastname: Oh
authors.forename: Kwang S
authors.initials: KS
authors.lastname: Lyoo
authors.forename: Kyung J
authors.initials: KJ
authors.lastname: Yoon
authors.forename: Yong H
authors.initials: YH
authors.lastname: Park
authors.forename: Bong K
authors.initials: BK
authors.lastname: Park
mesh_terms: D000818:Animals; D001483:Base Sequence; D004196:Disease Outbreaks; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology; D053122:Influenza A Virus, H3N2 Subtype; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007723:Korea / Q000453:epidemiology; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus