[Experimental study on effect of fuzheng yiliu decoction on tumor cell cycle and telomerease].
OBJECTIVE
To study the mechanism of anti-tumor effect of Fuzheng Yiliu Decoction (FZYLD).


METHODS
S180 neoplasm strain was inoculated in Kunming mice to establish model of S180 solid tumor. The model animals were treated with FZYLD by gastrogavage, the cell cycle of tumor were checked up by flow cytometer and the telomerease kit was used to test telomerease activity.


RESULTS
The stage G0/1 ratio of tumor cells in model animals treated with FZYLD increased, while cells of S stage decreased, with telomerease activity inhibited. These changes were different significantly from those in the model animals treated with normal saline (P < 0.001).


CONCLUSIONS
FZYLD could block the tumor cell proliferation procedure and inhibit the DNA synthesis and duplication in tumor cell. And the suppression of telomerease activity might be one of the mechanisms affecting the tumor cell proliferation cycle.
Dai
E L
EL
Institute of Integrated Traditional and Westen Medicine of Lanzhou Medical College, Lanzhou 730000.
Zhao
J X
JX
Zhu
Y Z
YZ
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D008297:Male; D051379:Mice; D012510:Sarcoma 180 / Q000201:enzymology* / Q000473:pathology*; D019098:Telomerase / Q000378:metabolism*
D000972:Antineoplastic Agents, Phytogenic; D004273:DNA, Neoplasm; D004365:Drugs, Chinese Herbal; D019098:Telomerase