[Experimental study of protective effect of tongxinluo capsule on isoproterenol induced myocardial injury in rats].
OBJECTIVE
To study the protective effect of Tongxinluo capsule (TXLC) on myocardial injury induced by isoproternol.


METHODS
Myocardial injury was induced in 34 rats by subcutaneous injection of isoproterenol (85 mg/kg). The experimental animals were randomly divided into the control group, isoproterenol group and TXLC group. The histopathological change of myocardia was investigated by HE staining, the myocardial cell apoptosis were observed by TUNEL method and the ultrastructure of myocardial cell examined by electron microscope.


RESULTS
No cell degeneration and necrosis, only very few cells of apoptosis positive were found in the control group. While in the isoproterenol group, marked necrosis of myocardial tissue and increase of apoptosis cells were found, and characteristic changes of cell apoptosis were observed under electron microscope. After TXLC treatment, the myocardial necrosis and cell apoptosis were markedly alleviated.


CONCLUSIONS
Isoproterenol could induce myocardial necrosis and apoptosis, TXLC could alleviate the myocardial injury through preventing myocardial cell necrosis and apoptosis.
Zhao
M Z
MZ
Department of Internal Medicine, Xuanwu Hospital, Capital Medical University, Beijing 100053.
Liu
L
L
Wang
J R
JR
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002214:Capsules; D009202:Cardiomyopathies / Q000139:chemically induced / Q000473:pathology*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007545:Isoproterenol; D008297:Male; D009206:Myocardium / Q000473:pathology*; D009336:Necrosis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007545:Isoproterenol