title: Collision-activated infrared multiphoton dissociation in a quadrupole ion trap mass spectrometer.
abstract: We propose and demonstrate a new method for multiple-stage mass spectrometry (MSn), collision-activated infrared multiphoton dissociation (CA-IRMPD), which is very effective for the quadrupole ion trap mass spectrometer (QITMS). CA-IRMPD uses a combination of focused laser irradiation (beam radius, approximately 0.4 mm) and collisional activation by a supplemental AC voltage between endcap electrodes. This combination enables IRMPD, which has conventionaLly been ineffective above 10(-4) Torr, to be used under a standard bath gas pressure of 2-8 mTorr. CA-IRMPD can produce richer spectra of product ions than CID or IRMPD while maintaining high sensitivity and mass resolution; thus, it will contribute to an accurate determination of peptide sequences.
authors.affiliation: Central Research Laboratory, Hitachi, Ltd., Tokyo, Japan. h-yuichi@crl.hitachi.co.jp
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Hideki
authors.initials: H
authors.lastname: Hasegawa
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Yoshinari
authors.forename: Izumi
authors.initials: I
authors.lastname: Waki