Determination of D-amino acid oxidase activity in tumour cells.
BACKGROUND
We evaluated the assay for determining D-amino acid oxidase (DAAO) activity in tumour cells, rat liver and rat kidney for studying the effects of D-amino acid-containing solution on cancer patients.


RESULTS
In this method the amount of ammonia produced by the DAAO activity after removal of endogenous ammonia using a Sephadex G25 column was determined. The highest activity was observed in rat kidney, which was almost eight times that found in rat liver. As compared with host tissues, the DAAO activity in tumour cells was considerably less.


CONCLUSIONS
This DAAO assay may be useful for analysis of various tissue samples as well as tumour cells.
Sasamura
Taizo
T
Pharmaceutical Research Laboratories, Ajinomoto Co Inc, 1-1 Suzuki-cho, Kawasaki-ku Kawasaki, 210-8681 Japan.
Matsuda
Akihiko
A
Kokuba
Yukifumi
Y
D000594:Amino Acid Oxidoreductases / Q000032:analysis* / Q000378:metabolism*; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000201:enzymology*; D051381:Rats; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000641:Ammonia; D000594:Amino Acid Oxidoreductases