[The enhancing effect of electroporation and iontophoresis on the permeation of insulin through human skin].
OBJECTIVE
To study the enhancing effect of electroporation and iontophoresis on the permeation of insulin through human cadaver skin in vitro.


METHODS
Using side by side two-chamber diffusion cells, the flux of insulin achieved with iontophoresis and electrophoration were compared.


RESULTS
The application of high-voltage pulse combined with iontophoresis resulted in higher flux transdermal permeation of insulin than either one technique alone (P < 0.05). Pulsing at a higher voltage increased the flux of insulin more dramatically than pulsing at a lower voltage (P < 0.01). The transdermal transport of insulin by 90 pulse of 500 V (exponential pulse generater, pulse time: 20-24 ms, pulse frequency: 3 pulse.min-1) followed by iontophoresis led to a quick input and a high steady flux.


CONCLUSIONS
Electroporation combined with iontophoresis can enhance the permeation of insulin significantly.
Pan
Yan
Y
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Zhao
Hui-ying
HY
Zheng
Jun-min
JM
D018274:Electroporation / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D007478:Iontophoresis / Q000379:methods*; D010539:Permeability; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption
D007328:Insulin