title: [Study on the metabolites of guanfu base A hydrochloride in rat urine by high performance liquid chromatograph-mass spectrum].
abstract: OBJECTIVE
  To study the metabolites of guanfu base A hydrochloride (GFA) in rat urine.
  
  
  METHODS
  Rat urine was collected after i.v. injection of GFA. Phase I metabolites were identified by HPLC/MS and by comparison with authentic standards. Phase II conjugates were treated with either glucuronidase or sulfatase in presence or absence of glucuronidase specific inhibitor D-saccharic acid beta-1,4-lactone. The aglycones were identified by HPLC/MS.
  
  
  RESULTS
  Phase I metabolites guanfu base I (GFI) and guanfu alcohol-amine (AA) were separated and identified in rat urine by comparison with authentic standards. Phase II conjugates, for which no authentic standards were available, GFA glucuronide and sulfate conjugates, GFI glucuronide and sulfate were separated and tentatively identified by hydrolysis with glucuronidase or sulfatase, the aglycones, GFA and GFI, were identified in rat urine.
  
  
  CONCLUSIONS
  After i.v. injection of GFA, GFA is metabolized into GFI, AA, GFA glucuronide and sulfate conjugates, GFI glucuronide and sulfate conjugates in rat urine. The polarity of the metabolites is increased, and the effectiveness of them is lower than the parent drug.
authors.affiliation: Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China. ajiye@public1.ptt.js.cn
authors.forename: Ji-ye
authors.initials: JY
authors.lastname: A
authors.forename: Guang-ji
authors.initials: GJ
authors.lastname: Wang
authors.forename: Xiao-quan
authors.initials: XQ
authors.lastname: Liu
authors.forename: Dan-yun
authors.initials: DY
authors.lastname: Jiang
authors.forename: Jing-han
authors.initials: JH
authors.lastname: Liu
mesh_terms: D031841:Aconitum / Q000737:chemistry; D000470:Alkaloids / Q000302:isolation & purification / Q000378:metabolism* / Q000652:urine; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006576:Heterocyclic Compounds, 4 or More Rings; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000470:Alkaloids; D006576:Heterocyclic Compounds, 4 or More Rings; C040615:Guan-fu base A