[Effect of MNNG on ornithine decarboxylase activity in cells from adult Schistosoma japonicum].
OBJECTIVE
To observe the change of ornithine decarboxylase(ODC) activity in cells from adult Schistosoma japonicum after the cells were treated with N-Methyl-N-Nitro-N-Nitrosoguanidine (MNNG).


METHODS
The cells were treated with MNNG at a concentration of 3 micrograms/ml for 48 hours after the cells being incubated for one week. The cells were then cultured with RPMI-1640 containing 10% calf serum. ODC activity was detected with spectrophotography.


RESULTS
ODC activity rose significantly in two to three weeks after the cells were treated with MNNG.


CONCLUSIONS
There was ODC activity in cells from adult S. japonicum and MNNG has an effect to reinforce ODC activity in the cells.
Fan
Hong
H
Department of Parasitology and Microbiology, Hubei Traditional Medical College, Wuhan 430061.
Huang
Min
M
Wang
Qian
Q
Li
Man-jun
MJ
D000818:Animals; D002478:Cells, Cultured; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D012549:Schistosoma japonicum / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology
D008769:Methylnitronitrosoguanidine; D009955:Ornithine Decarboxylase