title: Intracellular fibroblast growth factor produces effects different from those of extracellular application on development of avian cochleovestibular ganglion cells in vitro.
abstract: In an avian coculture system, the neuronal precursors of the cochleovestibular ganglion typically migrated from the otocyst and differentiated in response to soluble fibroblast growth factor (FGF-2), which had free access to FGF receptors on the cell surface. Free FGF-2 switched cells from a proliferation mode to migration, accompanied by increases in process outgrowth, fasciculation, and polysialic acid expression. Microsphere-bound FGF-2 had some of the same effects, but in addition it increased proliferation and decreased fasciculation and polysialic acid. As shown by immunohistochemistry, FGF-2 that was bound to latex microspheres depleted the FGF surface receptor protein, which localized with the microspheres in the cytoplasm and nucleus. For microsphere-bound FGF-2, the surface receptor-mediated responses to FGF-2 appear to be limited and the door opened to another venue of intracellular events or an intracrine mechanism.
authors.affiliation: Department of Neuroscience, University of Connecticut Health Center, Farmington, Connecticut, USA.
authors.forename: Masako M
authors.initials: MM
authors.lastname: Bilak
authors.forename: Waheeda A
authors.initials: WA
authors.lastname: Hossain
authors.forename: D Kent
authors.initials: DK
authors.lastname: Morest
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003051:Cochlea / Q000196:embryology / Q000294:innervation*; D018920:Coculture Techniques; D005110:Extracellular Space / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000494:pharmacology*; D007424:Intracellular Fluid / Q000378:metabolism; D008863:Microspheres; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D017468:Receptors, Fibroblast Growth Factor / Q000096:biosynthesis; D012995:Solubility; D013136:Spiral Ganglion / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014722:Vestibule, Labyrinth / Q000196:embryology / Q000294:innervation*
chemical_list: D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2