title: Evaluation of ELISA kit hypodermosis in serodiagnosis of Przhevalskiana silenus in goats and Cephalopenia titillator in camels.
abstract: Myiasis is a problem of Medical and Veterinary importance worldwide. It affects human welfare both directly and indirectly. ELISA kit hypodermosis was evaluated in detecting antibodies against two oestrid larvae, Przhevalskiana silenus in goats and Cephalopina titillator in camels. Both animals were parasitologically proved to have oestrid myiasis infestation. Antibodies against P. silenus were detected in 38 out of 40 (95%) infested goats and negative results among the control group. With C. titillator, antibodies were detected in 39 out of 40 (97.5%) infested camels. But one of the control (10%) gave false seropositivity. ELISA kit hypodermosis diagnosed antibodies against P. silenus in goats and C. titillator in camels. Consequently, there are cross reactions between anti-hypoderma antibodies and antibodies against both Przhevalskiana and Cephalopina. ELISA is confirmed to be an easy and economic tool in diagnosing myiasis infestation when direct examination is not a simple mean and/or for epidemiological studies. The whole results were discussed. Focusing on the clinical pictures and pathogenesis of human myiasis was included to clarify the zoonotic importance of myiasis in Egypt.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
authors.forename: T A
authors.initials: TA
authors.lastname: Morsy
authors.forename: A M
authors.initials: AM
authors.lastname: Farrag
authors.forename: S A
authors.initials: SA
authors.lastname: Mazyad
authors.forename: M M
authors.initials: MM
authors.lastname: Abou-Gamra
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood*; D002162:Camelus / Q000469:parasitology*; D004175:Diptera / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D015511:Goat Diseases / Q000175:diagnosis* / Q000469:parasitology; D006041:Goats / Q000469:parasitology; D007000:Hypodermyiasis / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D000906:Antibodies