[Inhibition of in vitro translation of esterase mRNA of dipterex-resistant mosquito (Culex pipiens pallens) by antisense nucleic acids].
OBJECTIVE
To examine the inhibitory effect of antisense nucleic acid on the in vitro translation of esterase mRNA from dipterex-resistant Culex pipiens pallens.


METHODS
18-mer nucleic acid was synthesized and complementary to the translation initiation site of mRNA of dipterex-resistant mosquitoes. The ODNs were annealed to the corresponding mRNA molecules and they were added to rabbit reticulocyte cell-free system. The translation products were analyzed by SDS-PAGE. After fixing, the gel was exposed to X-ray film by autoradiography for analysis of protein synthesis.


RESULTS
Six mumol/L of ODNs elicited a 50% reduction in specific protein expression, and 20 mumol/L of ODNs inhibited the expression of esterase by 80%. The SDS-PAGE showed that the band of reduced amounts of 65 kDa protein for resistant mosquito was almost the same as that for sensitive sample.


CONCLUSIONS
Antisense oligonucleic acids to the esterase mRNA of dipterex-resistant mosquito could effectively inhibit its in vitro translation.
Zhu
H M
HM
Department of Etiologic Biology, Second Military Medical University, Shanghai 200433.
Zou
Y D
YD
D000818:Animals; D003465:Culex / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance / Q000235:genetics; D004950:Esterases / Q000235:genetics*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D014236:Trichlorfon / Q000494:pharmacology
D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D014236:Trichlorfon; D004950:Esterases