Collaborative competition mechanism for gene activation in vivo.
The mechanism by which gene regulatory proteins gain access to their DNA target sites is not known. In vitro, binding is inherently cooperative between arbitrary DNA binding proteins whose target sites are located within the same nucleosome. We refer to such competition-based cooperativity as collaborative competition. Here we show that arbitrarily chosen foreign DNA binding proteins, LexA and Tet repressor, cooperate with an adjacently binding endogenous activator protein, Gcn4, to coactivate expression of chromosomal reporter genes in Saccharomyces cerevisiae. Coactivation requires that the cooperating target sites be within a nucleosome-length distance; it leads to increased occupancy by Gcn4 at its binding site; and it requires both Gcn5 and Swi/Snf which, at an endogenous Gcn4-dependent promoter, act subsequent to Gcn4 binding. These results imply that collaborative competition contributes to gene regulation in vivo. They further imply that, even in the presence of the cell's full wild-type complement of chromatin remodeling factors, competition of regulatory proteins with histone octamer for access to regulatory target sites remains a quantitative determinant of gene expression levels. We speculate that initial target site recognition and binding may occur via spontaneous nucleosomal site exposure, with remodeling factor action required downstream to lock in higher levels of regulatory protein occupancy.
Miller
Joanna A
JA
Department of Biochemistry, Molecular Biology and Cellular Biology, Northwestern University, Evanston, Illinois 60208-3500, USA.
Widom
Jonathan
J
D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001665:Binding Sites; D001667:Binding, Competitive; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005690:Galactose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D049452:Green Fluorescent Proteins; D051548:Histone Acetyltransferases; D008164:Luminescent Proteins / Q000378:metabolism; D008957:Models, Genetic; D009707:Nucleosomes / Q000378:metabolism; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013752:Tetracycline / Q000494:pharmacology; D014158:Transcription, Genetic; D000099160:LexA Repressor Protein; D000099224:p300-CBP-Associated Factor
D000255:Adenosine Triphosphate; D001426:Bacterial Proteins; D002843:Chromatin; D004247:DNA; D004268:DNA-Binding Proteins; D005690:Galactose; D005947:Glucose; D049452:Green Fluorescent Proteins; D051548:Histone Acetyltransferases; D008164:Luminescent Proteins; D009707:Nucleosomes; D011494:Protein Kinases; D012313:RNA; D012333:RNA, Messenger; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases; D013752:Tetracycline; D000099160:LexA Repressor Protein; C034811:tetracycline resistance-encoding transposon repressor protein; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor