Approaches for the inhibition of human telomerase based on the use of peptide nucleic acids and hammerhead ribozymes.
The ability of peptide nucleic acids and hammerhead ribozymes, which target different subunits of human telomerase, to efficiently inhibit the enzyme's catalytic activity has been clearly demonstrated in several in vitro studies carried out in human immortalized and cancer cell lines. However, the actual efficacy of these molecules still needs to be validated in in vivo human tumor models, and such validation appears to be largely dependent on the development of reliable systems for their intracellular delivery.
Zaffaroni
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Dipartimento di Oncologia Sperimentale, Istituto Nazionale per lo Studio e la Cura dei, Tumori, 20133 Milan, Italy. zaffaroni@istitutotumori.mi.it
Villa
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Pennati
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Folini
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D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D020135:Peptide Nucleic Acids / Q000494:pharmacology*; D016337:RNA, Catalytic / Q000494:pharmacology*; D019098:Telomerase / Q000037:antagonists & inhibitors*
D017931:DNA Primers; D004791:Enzyme Inhibitors; D020135:Peptide Nucleic Acids; D016337:RNA, Catalytic; D019098:Telomerase