[Preparation of DNA probe for Cryptosporidium parvum].
OBJECTIVE
To prepare a probe with high specificity and sensitivity for the detection of Cryptosporidium parvum.


METHODS
Using PCR method, a fragment from the DNA of C. parvum was amplified. The PCR product, 452 bp DNA, was labeled with hapten digoxigenin.


RESULTS
Examination of sensitivity showed that the DNA probe could detect as low as 2 pg DNA from C. parvum. The dot-blot hybridizition assay showed that the probe hybridized with the DNA of C. parvum, but not hybridized with DNA of E. histolytica, G. lamblia, E. coli and D. bacilli.


CONCLUSIONS
The probe was highly specific and sensitive for the detection of C. parvum.
Guo
B
B
Department of parasitology, Chagzhi Medical College, Changzhi 046000.
Zhang
J
J
Lian
D
D
D000818:Animals; D016785:Cryptosporidium parvum / Q000235:genetics / Q000302:isolation & purification*; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D015342:DNA Probes; D016054:DNA, Protozoan