[In vitro biocompatibility and degradation of nacre/polylactic acid composite artificial bone].
OBJECTIVE
To investigate the in vitro biocompatibility and degradation of nacre/polylactic acid composite artificial bone (NPCB) grafts.


METHODS
Human osteoblast cells were cultured with NPCB discs for observation of the morphological features and cell proliferation by optical microscope and scanning electron microscope. The mass loss of NPCB discs and pH variations of the saline in which the discs were immersed were examined every 2 weeks in a course of 16 weeks in vitro.


RESULTS
Compared with the negative control group, NPCB showed no visible cytotoxicity and facilitated the growth and proliferation of the osteoblast cells. As the immersion prolonged, gradual decrease in the mass of NPCB was observed accompanied by regular pH alteration of the saline.


CONCLUSIONS
NPCB possesses good in vitro biocompatibility and may be gradually degraded.
Liu
Jin-biao
JB
Department of Orthopaedics Surgery, Chengdu General Hospital of Chengdu Command, Chengdu 610083, China.
Chen
Jian-ting
JT
Jin
Da-di
DD
Quan
Yi
Y
Fan
Wen-wen
WW
D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D018786:Bone Substitutes / Q000737:chemistry* / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry* / Q000378:metabolism; D011091:Polyesters; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D019736:Prostheses and Implants
D001672:Biocompatible Materials; D018786:Bone Substitutes; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)