title: Steric-control for the enantioselective hydrolysis of amino acid esters in hybrid membrane systems.
abstract: The enantioselective hydrolysis of the amino acid esters, p-nitrophenyl-N-dodecanoyl-D(L)-phenylalaninates (C(12)-D(L)-Phe-PNP) catalyzed by active tripeptide, N-(benzyloxycarbonyl)-L-phenylalanyl-L-histidyl-L-leucine (Z-PheHisLeu) in the presence of coaggregates (hybrid membranes) composed of native phospholipid, L-alpha-dimyristoylphosphatidylcholine (DMPC) and nonionic surfactant, polyoxyethylene (8) lauryl ether (C(12)(EO)(8)) was easily controlled by regulating the reaction temperature and changing the composition of coaggregates. Furthermore, excellent correlations were observed between the enantioselectivity in the hydrolysis of C(12)-D(L)-Phe-PNP catalyzed by Z-PheHisLeu in the presence of coaggregates and physical properties of hybrid membranes. It is assumed that catalytic activities of tripeptide catalyst in hybrid membranes should be regulated by changing the microenvironments of reaction fields.
authors.affiliation: Department of Applied Life Science, Sojo University, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Tanoue
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ichihara
authors.forename: Koichi
authors.initials: K
authors.lastname: Goto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Ueoka
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism*; D004952:Esters / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D008567:Membranes, Artificial; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D004952:Esters; D008567:Membranes, Artificial