[mRNA expressions of voltage-dependent potassium channels in the brain of scopolamine-induced memory impaired rats].
OBJECTIVE
To study mRNA expression difference of voltage-dependent potassium channels in the brain of scopolamine-induced memory impaired rats.


METHODS
Memory impairments induced in rats by scopolamine (1 mg.kg-1) were assessed in the Morris water maze test. After rats were injected intraperitoneally with scopolamine for 6 days, the mRNA expression level of five voltage-dependent potassium channels, Kv1.4, Kv1.5, Kv2.1, Kv4.2 and Kv4.3 were detected in the rat cortex and hippocampus by RT-PCR.


RESULTS
Scopolamine (1 mg.kg-1) was shown to significantly induce memory impairment in rats. The mRNA levels of Kv4.2 were decreased by 28.8% and 33.9% in the cortex and hippocampus, respectively. The mRNA levels of Kv1.4 and Kv2.1 were increased in the hippocampus by 111.7% and 64.3%, respectively. There were no differences in the brain mRNA levels of other voltage-dependent potassium channels in scopolamine-induced memory impaired rat.


CONCLUSIONS
The mRNA expression levels of voltage-dependent potassium channels changed significantly in the brain of scopolamine-induced memory impaired rats.
Xu
Xiang-hua
XH
Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.
Pan
Ya-ping
YP
Wang
Xiao-liang
XL
D000759:Adjuvants, Anesthesia; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D018782:Maze Learning; D008569:Memory Disorders / Q000139:chemically induced / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012601:Scopolamine
D000759:Adjuvants, Anesthesia; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger; D012601:Scopolamine