A sense of closeness: protein detection by proximity ligation.
Highly specific and sensitive procedures will be required to evaluate proteomes. Proximity ligation is a recently introduced mechanism for protein analysis. In this technique, the convergence of sets of protein-binding reagents on individual target molecules juxtaposes attached nucleic acid sequences. Through a ligation reaction a DNA reporter sequence is created, which can be amplified. The procedure thus encodes detected proteins as specific nucleic acid sequences in what may be viewed as a reverse translation reaction.
Gullberg
Mats
M
The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, Se-751 85 Uppsala, Sweden.
Fredriksson
Simon
S
Taussig
Michael
M
Jarvius
Jonas
J
Gustafsdottir
Sigrun
S
Landegren
Ulf
U
D001483:Base Sequence; D015342:DNA Probes; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D046911:Macromolecular Substances; D015336:Molecular Probe Techniques; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D009841:Oligonucleotides / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D020539:Sequence Analysis, Protein / Q000379:methods*
D015342:DNA Probes; D046911:Macromolecular Substances; D009841:Oligonucleotides; D011506:Proteins