title: Impala, a transposon from Fusarium oxysporum, is active in the genome of Penicillium griseoroseum.
abstract: An autonomous impala transposon trapped in Fusarium oxysporum by insertion within the niaD gene encoding nitrate reductase was introduced in the genome of the fungus Penicillium griseoroseum, a producer of pectinase enzymes. Through a phenotypic assay, we demonstrate that this element is able to excise from the niaD gene and to reinsert at new genomic positions. As in the original host, impala inserts into a TA site and footprints left by impala excisions are generally 5 bp. The fact that impala is able to transpose in P. griseoroseum offers the opportunity to develop a gene-tagging system based on this element with the objective to detect and clone genes related in pectinase production.
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa, 36571-000, MG, Viçosa, Brazil. mvqueiro@mail.ufv.br
authors.forename: Marisa Vieira
authors.initials: MV
authors.lastname: de Queiroz
authors.forename: Marie Josée
authors.initials: MJ
authors.lastname: Daboussi
mesh_terms: D015139:Blotting, Southern; D004251:DNA Transposable Elements / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D005670:Fusarium / Q000235:genetics*; D016681:Genome, Fungal; D010407:Penicillium / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004271:DNA, Fungal