[DNA profiling of Pogostemon cablin chemotypes differing in essential oil composition].
OBJECTIVE
To provide molecular evidence for quality evaluation and GAP production of Pogostemon cablin (Blanco) Benth. cultivated in different regions in Guangdong and Hainan provinces, China, by comparing two sequences (1.2 kb of plastid matK gene and 1.8 kb of nuclear 18S rRNA gene) and two chemotypes (Pogostone-type and Patchouliol-type in essential oil composition).


METHODS
PCR direct sequencing was applied to detemine the matK and 18S rRNA sequences for six samples of Pogostemon cablin from different localities.


RESULTS
The matK sequences of six samples of Pogostemon cablin from different regions of cultivation are 1,245 bp in length, which coding 415 amino acids of protein (maturase), and 18S rRNA sequences are 1,803-1,805 bp in size. Based on multiple sequence alignment, there are 47 variable sites in the matK sequence of these six samples, 17 in the 18S rRNA sequence. The cluster tree reconstructed by UPGMA method shows that the sequence divergence both in matK and 18S rRNA genes among six samples of Pogostemon cablin was well correlative with their regions of cultivation and intraspecific chemotypes of essential oil composition.


CONCLUSIONS
Combining with chemical and biogeographical data, DNA sequencing can become a powerful tool in the key technique-species identification of quality evaluation and GAP production of Pogostemon cablin.
Liu
Yu-ping
YP
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Luo
Ji-peng
JP
Feng
Yi-fan
YF
Guo
Xiao-ling
XL
Cao
Hui
H
D001483:Base Sequence; D018744:DNA, Plant / Q000032:analysis; D004722:Endoribonucleases / Q000235:genetics*; D017343:Genes, Plant; D019686:Lamiaceae / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D009822:Oils, Volatile / Q000032:analysis; D011110:Polymorphism, Genetic; D011786:Quality Control; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D018744:DNA, Plant; D009822:Oils, Volatile; D009713:Nucleotidyltransferases; C034038:mRNA maturase; D004722:Endoribonucleases