title: Urinary heat shock protein-72 excretion in clinical and experimental renal ischemia.
abstract: Renal ischemia not only causes injury but also induces repair mechanisms, such as the cellular induction of the 72-kilodalton heat shock protein HSP-72. The aim of this study was to determine whether HSP-72 is excreted in urine after ischemic renal injury. The first urine of six pediatric allograft recipients was examined for proteinuria and urinary HSP-72 excretion. Sprague-Dawley rats were treated with renal ischemia or hyperthermia and renal cortex and urinary HSP-72 levels were determined. HSP-72 was excreted in the first urine of renal allografts. In rats, renal HSP-72 was induced both by renal ischemia or hyperthermia. However, only renal ischemia resulted in urinary excretion of HSP-72. Urinary excretion of HSP-72 indicates an increased renal stress response and loss of tubular cell integrity after clinical and experimental renal ischemia.
authors.affiliation: Department of Pediatrics, Kinderdialyse, AKH Wien, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Mueller
authors.forename: Bettina
authors.initials: B
authors.lastname: Bidmon
authors.forename: Patrick
authors.initials: P
authors.lastname: Pichler
authors.forename: Klaus
authors.initials: K
authors.lastname: Arbeiter
authors.forename: Dagmar
authors.initials: D
authors.lastname: Ruffingshofer
authors.forename: Scott K
authors.initials: SK
authors.lastname: VanWhy
authors.forename: Christoph
authors.initials: C
authors.lastname: Aufricht
mesh_terms: D000818:Animals; D002648:Child; D002675:Child, Preschool; D005260:Female; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000652:urine*; D006801:Humans; D007511:Ischemia / Q000652:urine*; D007668:Kidney / Q000098:blood supply*; D016030:Kidney Transplantation; D008297:Male; D011507:Proteinuria / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D014184:Transplantation, Homologous
chemical_list: D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins