[Development of Acute Promyelocytic Leukemia in PML-RARalpha Transgenic Mice]
To investigate the leukemogenic potential of PML-RARalpha fusion protein in vivo, hCG-PML-RARalpha transgene was constructed using molecular cloning technique and hCG-PML-RARalpha transgenic mice were generated. The genotype and phenotype of hCG-PML-RARalpha transgenic mice were analyzed by PCR, RT-PCR, morphology of peripheral blood and bone marrow cells, and pathological examination of spleen, liver and bone marrow. As a result, acute promyelocytic leukemia was developed in 3 hCG-PML-RARalpha transgenic mice in 1 - 5 months. The results demonstrated that PML-RARalpha fusion protein plays a crucial role in leukemogenesis.
Meng
Xiuqin
X
Shanghai Institute of Hematology, Affiliated Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China; Shanghai Railway Center Hospital, Shanghai 200072, China.
Cheng
Guoxiang
G
Cao
Wenjun
W
Zhu
Jiang
J
Chen
Jianquan
J
Chen
Zhu
Z
Chen
Saijuan
S