[Comparative effect of different media in in vitro cultivation of Trichomonas vaginalis].
OBJECTIVE
To investigate the optimal condition for in vitro cultivation of Trichomonas vaginalis for obtaining a better harvest of T. vaginalis.


METHODS
An isolate of T. vaginalis from clinical specimens was cultivated in three different media with initial inoculation of 9.0 x 10(4)/ml under pH 5.6.


RESULTS
There was distinct difference after 96 h incubation in the cumulative harvest of T. vaginalis. The highest harvest was received in cysteine/liver/peptone/maltose medium, followed by the liver/peptone/maltose medium and soybean/liver/peptone/maltose medium.


CONCLUSIONS
The cysteine/liver/peptone/maltose medium may be a suitable environment for in vitro multiplication of T. vaginalis.
Geng
Zhi-hui
ZH
Department of the Third Centre, Norman Bethune University of Medical Sciences, Changchun 130021.
Han
Shu-mei
SM
Liu
Li
L
Shi
Yu-lu
YL
Li
Shu-hong
SH
D000818:Animals; D003470:Culture Media; D014246:Trichomonas vaginalis / Q000254:growth & development*
D003470:Culture Media