title: Rapid generation of inducible mouse mutants.
abstract: We have generated an optimized inducible recombination system for conditional gene targeting based on a Cre recombinase-steroid receptor fusion. This configuration allows efficient Cre-mediated recombination in most organs of the mouse upon induction, without detectable background activity. An ES cell line, was established that carries the inducible recombinase and a loxP-flanked lacZ reporter gene. Out of this line, completely ES cell-derived mice were efficiently produced through tetraploid blastocyst complementation, without the requirement of mouse breeding. Our findings provide a new concept allowing the generation of inducible mouse mutants within 6 months, as compared to 14 months using the current protocol.
authors.affiliation: ARTEMIS Pharmaceuticals GmbH, Neurather Ring 1, 51063 Cologne, Germany.
authors.forename: Jost
authors.initials: J
authors.lastname: Seibler
authors.forename: Branko
authors.initials: B
authors.lastname: Zevnik
authors.forename: Birgit
authors.initials: B
authors.lastname: Küter-Luks
authors.forename: Susanne
authors.initials: S
authors.lastname: Andreas
authors.forename: Heidrun
authors.initials: H
authors.lastname: Kern
authors.forename: Thomas
authors.initials: T
authors.lastname: Hennek
authors.forename: Anja
authors.initials: A
authors.lastname: Rode
authors.forename: Cornelia
authors.initials: C
authors.lastname: Heimann
authors.forename: Nicole
authors.initials: N
authors.lastname: Faust
authors.forename: Gunther
authors.initials: G
authors.lastname: Kauselmann
authors.forename: Michael
authors.initials: M
authors.lastname: Schoor
authors.forename: Rudolf
authors.initials: R
authors.lastname: Jaenisch
authors.forename: Klaus
authors.initials: K
authors.lastname: Rajewsky
authors.forename: Ralf
authors.initials: R
authors.lastname: Kühn
authors.forename: Frieder
authors.initials: F
authors.lastname: Schwenk
mesh_terms: D000818:Animals; D002460:Cell Line; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics*; D008822:Mice, Transgenic; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D011987:Receptors, Steroid; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases