[Obtaining diagnostic fluorescent monoclonal immunoglobulins against the V. cholerae 0139-serovar].
Below is given a procedure of the obtaining diagnostic fluorescent monoclonal immunoglobulin to detect cholera vibrios of O139 serovar. While obtaining preparations it was managed to determine optimal FTTS-MKA ratio, duration of their conjugation, series of fluorochrome. Test specimens of fluorescent monoclonal immunoglobulin provides intensive glow of V cholerae O139 cells in the working dilution 1:16-1:32. Tests of diagnostic FTTS-MKA on the great number of homologic and heterologic strains showed their strict specificity and high sensibility as to cholera vibrios of O139 serogroup.
Alekseeva
L P
LP
Mazrukho
B L
BL
Markina
O V
OV
Chemisova
O S
OS
Sal'nikova
O I
OI
Ishina
E V
EV
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007136:Immunoglobulins; D019081:O Antigens / Q000032:analysis / Q000276:immunology*; D012680:Sensitivity and Specificity; D012703:Serotyping; D014734:Vibrio cholerae / Q000276:immunology* / Q000302:isolation & purification; D014871:Water Microbiology
D000911:Antibodies, Monoclonal; D007136:Immunoglobulins; D019081:O Antigens