[Studies on the specificity of target organs and repair dynamics of DNA damage induced by 1,2-dichloroethane].
In order to investigate the genotoxicity of 1,2-dichloroethane (DCE) and to determine its carcinogenic targets, the DNA damage and repair dynamics were detected by using comet assay in the isolated nuclei with tissue-homogenization. Liver, lung, kidney, spleen, bone marrow, testicle, stomach, colon, bladder, and peripheral lymphocytes of mice were isolated at 3 h, 8 h, 24 h after DCE being gavaged. The results showed that: a) liver, lung, kidney, bone marrow, colon, stomach and peripheral lymphocytes were the genotoxic target organs of DCE in mice; b) DNA damage and repair dynamics were obviously different among the test organs, and the organs with DNA damage and slow repaired (lung, stomach and hemocyte) were in accordance with the carcinogenic targets. The results indicate that multiple organ comet assay in vivo is very useful for the evaluation of genotoxicity and prediction of carcinogenic target organ. The comet tail of peripheral lymphocyte is suitable as a biomarker for DNA damage in the target organs of living creatures and population exposed to DCE. It is suggested that in vivo comet assay is also very important to select right marker cells and biomarker in the epidemiological studies on genotoxicity.
Heng
Z
Z
School of Public Health, West China University of Medical Sciences, Chengdu 610041, China.
Li
A
A
Zhang
Z
Z
D000818:Animals; D015415:Biomarkers; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004785:Environmental Pollutants / Q000633:toxicity*; D005025:Ethylene Dichlorides / Q000633:toxicity*; D008214:Lymphocytes
D015415:Biomarkers; D004785:Environmental Pollutants; D005025:Ethylene Dichlorides; C024565:ethylene dichloride