[Determination on the glycosyl sequence of gypenoside A by TLC-FABMS].
The glycosyl sequence of gypenoside A (A) isolated from Gynostemma pentaphyllum Makino was determinated by TLC-FABMS. The gypenoside A was hydrolysed with cellulase and the hydrolysate showed eight spots on a TLC plate, and the FABMS analysis of these spots indicated they corresponded to gypenoside A, A-Rham, A-Glu, A-Rham-Glu, A-Rham-Glu, A-Glu-Glu, A-Rham-2Glu, A-Rham-2Glu in order. Two chains can be determined to be Rham-Glu, and Glu-Glu, respectively. So the glycosyl sequence of gypenoside A was identical of gypenoside XLIII.
Liu
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G
Guangzhou Pharmaceutical Industrial Research Institute, Guangzhou 510240.
Guo
D
D
Zhong
R
R
Yao
Z
Z
Wen
H
H
He
W
W
Hu
W
W
D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D029774:Gynostemma / Q000737:chemistry*; D006868:Hydrolysis; D008968:Molecular Conformation; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000032:analysis* / Q000737:chemistry*
D012503:Saponins; C117083:gypentonoside A