Effects of androgen-binding protein (ABP) on spermatid Tnp1 gene expression in vitro.
In vitro studies were designed to determine whether Sertoli cell-delivered ABP could act on spermatogenetic events, whether such an action could occur via a paracrine or a juxtacrine pathway and whether sex steroids could be involved in this action. ABP delivery to germ cells was achieved using an in vitro model based on recombinant rat ABP-producing mouse Sertoli cells cocultivated with rat spermatids. Using semi-quantitative RT-PCR, the expression of the Tnp 1 gene encoding the Transition Protein 1, involved in the histone to protamine replacement during spermatid nuclear transformation, was analyzed. Our results provide clear evidence that Sertoli cell-derived ABP acts on spermatids by modifying the TP1 mRNA level. This outcome, strictly requiring juxtacrine conditions, is obtained in the absence of sex steroid hormones. To our knowledge this is the first evidence of an effect of ABP itself on male germ cells.
Della-Maria
Julie
J
EA 3442 Génétique, Signalisation, Différenciation, Département de Cytologie, Histologie et Biologie du Développement, Faculté de Médecine, Université Henri Poincaré de Nancy, 9, avenue de la Forêt de Haye, BP 184, 54505 Vandoeuvre-lès-Nancy Cedex, France.
Gerard
Anne
A
Franck
Patricia
P
Gerard
Hubert
H
D000727:Androgen-Binding Protein / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics* / Q000378:metabolism; D018920:Coculture Techniques; D012739:Gonadal Steroid Hormones / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011917:Rats, Inbred Lew; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism; D013087:Spermatids / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*
D000727:Androgen-Binding Protein; D002868:Chromosomal Proteins, Non-Histone; D012739:Gonadal Steroid Hormones; C053886:spermatid transition proteins