Tissue response to a newly developed calcium phosphate cement containing succinic acid and carboxymethyl-chitin.
We developed a new calcium phosphate cement containing succinic acid and carboxymethyl-chitin in the liquid component. In this study, the biocompatibility and osteoconductivity of this new cement were investigated. After mixing, cement in putty form was implanted immediately between the periosteum and parietal bone and in the subcutaneous tissues of rats. In control cement, distilled water was used instead of the liquid component. In addition to histological evaluations, analyses with X-ray diffraction and Fourier transform infrared were performed for the subcutaneously implanted cements. Histological examination showed slight inflammation around the new cement on the bone and in the subcutaneous tissue at 1 week after surgery. At 2 weeks, the cement was partially bound to the parietal bone. The extent of the surface of the new cement directly in contact with the bone increased with time, and most of the undersurface of the new cement bound to the host parietal bone by 8 weeks. Analysis by X-ray diffraction showed that the new cement in the subcutaneous tissue was transformed into hydroxyapatite by 8 weeks. These results indicate that this new calcium phosphate cement is useful as a bone substitute material.
Yokoyama
Atsuro
A
Department of Oral Functional Science, Hokkaido University Graduate School of Dental Medicine, Kita-13 Nishi-7 Kita-ku, Sapporo 060-8586, Japan. yokoyama@den.hokudai.ac.jp
Matsuno
Hironobu
H
Yamamoto
Satoru
S
Kawasaki
Takao
T
Kohgo
Takao
T
Uo
Motohiro
M
Watari
Fumio
F
Nakasu
Masanori
M
D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D001843:Bone Cements / Q000737:chemistry* / Q000378:metabolism; D018786:Bone Substitutes; D002130:Calcium Phosphates; D002686:Chitin / Q000737:chemistry / Q000378:metabolism*; D005549:Foreign-Body Reaction; D007249:Inflammation / Q000209:etiology*; D008297:Male; D008422:Materials Testing; D010012:Osteogenesis / Q000502:physiology; D010294:Parietal Bone / Q000166:cytology / Q000378:metabolism; D010521:Periosteum / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared; D019802:Succinic Acid / Q000032:analysis*; D014961:X-Ray Diffraction
D001672:Biocompatible Materials; D001843:Bone Cements; D018786:Bone Substitutes; D002130:Calcium Phosphates; D002686:Chitin; D019802:Succinic Acid