[Study on effect of qingkailing injection and its active principle in inducing cell apoptosis in human acute promyelocytic leukemia].
OBJECTIVE
To investigate the mechanism of Qingkailing (QKL) Injection in treating acute promyelocytic leukemia.


METHODS
Using MTT technique, cell morphologic method, DNA gel electrophoresis and flow-cytometry to study the human acute promyelocytic leukemia (HL-60) cell apoptosis induced by QKL and its active principle.


RESULTS
QKL and its active principle, Baicalin and hyodeoxycholic acid, showed strong cytotoxicity in inhibiting HL-60 cell, the Bezoar cholic acid showed a weaker effect. Apoptosis could be induced after being treated for 6 hrs by the former three principles, showing a typical apoptosis peak under flow-cytometry, but could not be induced by the latter one.


CONCLUSIONS
QKL could induce leukemia cell apoptosis in vitro, which may be one of the mechanisms of QKLI in curing acute promyelocytic leukemia.
Chen
Z T
ZT
Affiliated Hospital of Shandong University of TCM, Jinan 250011.
Dong
Q
Q
Zhang
L
L
D017209:Apoptosis / Q000187:drug effects*; D003840:Deoxycholic Acid / Q000302:isolation & purification / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D018922:HL-60 Cells / Q000473:pathology; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D003840:Deoxycholic Acid; C038044:baicalin; C010471:hyodeoxycholic acid