Isozymes in Aegilops kotschyi and Ae. biuncialis x Secale cereale hybrids and Ae. kotschyi x S. cereale amphiploids in relation to their parents.
Seven enzymatic systems in F1 Aegilops kotschyi and Ae. biuncialis x Secale cereale hybrids, Aegilops kotschyi x S. cereale amphiploids and their parental species (Ae. kotschyi, Ae. biuncialis and S. cereale) were analysed by starch and polyacrylamide gel electrophoresis. Five of them (phosphoglucose isomerase, glutamic oxalacetic transaminase, esterase, acid phosphatase, and diaphorase) were polymorphic and two (malic dehydrogenase and superoxide dismutase) were monomorphic. Several isophorms of phosphoclucose isomerase, esterase, acid phosphatase, and diaphorase were detected in some hybrids and amphiploids, but absent in the parents. The role of regulators, translocations and recombination is discussed in relation to the origin of these new isophorms. Some parental isozymes were absent both in hybrids and amphiploids, probably as a result of the suppression of structural genes in new combinations of the three genomes.
Kalinowski
Andrzej
A
Institute of Plant Genetics, Polish Academy of Sciences, ul. Strzeszyńska 34, 60-479 Poznań, Poland. akal@igr.poznan.pl
Wojciechowska
Barbara
B
Borzyszkowska
Elzbieta
E
D000135:Acid Phosphatase / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D001219:Aspartate Aminotransferases / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D003433:Crosses, Genetic; D008058:Dihydrolipoamide Dehydrogenase / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D002523:Edible Grain / Q000201:enzymology* / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D006824:Hybridization, Genetic; D007527:Isoenzymes / Q000032:analysis* / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D008970:Molecular Weight
D007527:Isoenzymes; D008058:Dihydrolipoamide Dehydrogenase; D001219:Aspartate Aminotransferases; D004950:Esterases; D000135:Acid Phosphatase; D005956:Glucose-6-Phosphate Isomerase