Dietary vitamin E supplementation affects tissue lipid peroxidation of hybrid tilapia, Oreochromis niloticus x O. aureus.
A feeding trial was conducted to evaluate the effects of dietary vitamin E contents on the growth, ascorbate induced iron-catalyzed lipid peroxidation in post-mortem muscle and liver tissue, and Raman spectral changes in lens of juvenile hybrid tilapia (Oreochromis niloticus x O. aureus). Experimental fish were fed practical diets supplemented with 0, 50, 100, 200, 450 and 700 mg alpha-tocopheryl acetate/kg diet for 14 weeks. There was no significant difference in weight gain, feed conversion ratio and protein efficiency ratio among fish fed test diets (P>0.05). Protein content of fish fed diet containing the lowest vitamin E level was the lowest (P<0.05) among all groups. No difference was found in other body constituents among test fish (P>0.05). The thiobarbituric acid-reactive substances produced by iron-catalyzed lipid peroxidation in muscle and liver tissue of fish fed the diet without alpha-tocopheryl acetate supplementation were significantly (P<0.05) greater than those from fish fed diets containing higher levels of alpha-tocopheryl acetate. Dietary vitamin E supplementation increased the antioxidant capability of tilapia tissues against lipid peroxidation. Further, dietary vitamin E supplementation also influenced the lens cortical membrane structure of tilapia.
Huang
Chen-Huei
CH
Department of Aquatic Biosciences, National Chiayi University, 300 University Road, Chiayi, Taiwan, ROC. chuhuang@mail.ncyu.edu.tw
Chang
Ray-Jane
RJ
Huang
Sue-Lan
SL
Chen
Wenlung
W
D000818:Animals; D004032:Diet; D019587:Dietary Supplements; D006824:Hybridization, Genetic; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000737:chemistry; D018482:Muscle, Skeletal / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D017210:Tilapia / Q000145:classification / Q000378:metabolism*; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology*; D015430:Weight Gain / Q000187:drug effects
D014810:Vitamin E