Monitoring changes in anthocyanin and steroid alkaloid glycoside content in lines of transgenic potato plants using liquid chromatography/mass spectrometry.
Transgenic potato plants overexpressing and repressing enzymes of flavonoids biosynthesis were created and analyzed. The selected plants clearly showed the expected changes in anthocyanins synthesis level. Overexpression of a DNA encoding dihydroflavonol 4-reductase (DFR) in sense orientation resulted in an increase in tuber anthocyanins, a 4-fold increase in petunidin and pelargonidin derivatives. A significant decrease in anthocyanin level was observed when the plant was transformed with a corresponding antisense construct. The transformation of potato plants was also accompanied by significant changes in steroid alkaloid glycosides (SAG) level in transgenic potato tuber. The changes in SAGs content was not dependent on flavonoid composition in transgenic potato. However, in an extreme situation where the highest (DFR11) or the lowest (DFRa3) anthocyanin level was detected the positive correlation with steroid alkaloid content was clearly visible. It is suggested that the changes in SAGs content resulted from chromatin stressed upon transformation. A liquid chromatography/mass spectrometry (LC/MS) system with electrospray ionization was applied for profiling qualitative and quantitative changes of steroid alkaloid glycosides in tubers of twelve lines of transgenic potato plants. Except alpha-chaconine and alpha-solanine, in the extracts from dried tuber skin alpha-solamargine and alpha-solasonine, triglycosides of solasonine, were identified in minor amounts, triglycosides of solanidine dehydrodimers were also recognized.
Stobiecki
Maciej
M
Institute of Bioorganic Chemistry PAS, Noskowskiego 12/14, 61-704 Poznań, Poland. mackis@ibch.poznan.pl
Matysiak-Kata
Iwona
I
Frański
Rafał
R
Skała
Jacek
J
Szopa
Jan
J
D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism; D000470:Alkaloids / Q000032:analysis*; D000872:Anthocyanins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006027:Glycosides / Q000032:analysis*; D015394:Molecular Structure; D019441:Plant Epidermis / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D030821:Plants, Genetically Modified; D011198:Solanum tuberosum / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013256:Steroids / Q000032:analysis*
D000470:Alkaloids; D000872:Anthocyanins; D006027:Glycosides; D013256:Steroids; D000429:Alcohol Oxidoreductases; C056570:dihydroflavanol 4-reductase