title: Detection of Helicobacter pylori in paraffin-embedded and in shock-frozen gastric biopsy samples by fluorescent in situ hybridization.
abstract: We report on the successful application of fluorescent in situ hybridization for detection of Helicobacter pylori and determination of its clarithromycin susceptibility in formalin-fixed and paraffin-embedded gastric biopsy specimens that had been prepared for pathological examination. This method is useful when results from conventional culturing with antibiotic susceptibility testing are not available.
authors.affiliation: Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians-Universität München, 80336 Munich, Germany. ruessmann@m3401.mpk.med.uni-muenchen.de
authors.forename: Holger
authors.initials: H
authors.lastname: Rüssmann
authors.forename: Anne
authors.initials: A
authors.lastname: Feydt-Schmidt
authors.forename: Kristin
authors.initials: K
authors.lastname: Adler
authors.forename: Daniela
authors.initials: D
authors.lastname: Aust
authors.forename: Almuth
authors.initials: A
authors.lastname: Fischer
authors.forename: Sibylle
authors.initials: S
authors.lastname: Koletzko
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001706:Biopsy; D017291:Clarithromycin / Q000494:pharmacology; D016481:Helicobacter Infections / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000187:drug effects / Q000302:isolation & purification*; D006652:Histological Techniques; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008826:Microbial Sensitivity Tests; D016612:Paraffin Embedding; D012680:Sensitivity and Specificity; D013270:Stomach / Q000382:microbiology* / Q000473:pathology
chemical_list: D000900:Anti-Bacterial Agents; D017291:Clarithromycin