A new murine monoclonal antibody against Kx protein.
Mice immunized with a synthetic peptide located on an intracellular segment of the polytopic Kx protein (37 kDa) from human red blood cells (RBCs) produced a monoclonal antibody called C7B8. As expected, this antibody did not agglutinate common RBCs but reacted with permeabilized cells in flow cytometry. C7B8 recognizes the Kx protein on Western blots. Cross-reactivity of C7B8 with human calpain of human muscle extracts was demonstrated by Western blot analysis. This cross-reactivity precludes the use of C7B8 for Kx tissue distribution studies, but immobilized C7B8 was a convenient tool for purification of the Kell-Kx complex from RBC membrane extract by immunochromatography.
Hattab
C
C
INSERM U76, Institut National de la Transfusion Sanguine, Paris, France.
Blanchard
D
D
Gane
P
P
Verkarre
V
V
Petit-Leroux
Y
Y
Loirat
M-J
MJ
Cartron
J-P
JP
Bertrand
O
O
D026921:Amino Acid Transport Systems, Neutral / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D000937:Antigen-Antibody Reactions; D015153:Blotting, Western; D002154:Calpain / Q000276:immunology; D002846:Chromatography, Affinity; D003429:Cross Reactions; D005434:Flow Cytometry; D006801:Humans; D007626:Kell Blood-Group System
D026921:Amino Acid Transport Systems, Neutral; D000911:Antibodies, Monoclonal; D007626:Kell Blood-Group System; C087883:XK protein, human; D002154:Calpain