Intense light exposure changes the crystallin content in retina.
Toward a better understanding of light-induced photoreceptor damage, the crystallin content of rat retina was examined following intense light exposure. Nine crystallin species were identified by mass spectrometric analysis of rat retina fractionated by 2D gel electrophoresis. The Coomassie blue staining intensity of all crystallin 2D gel components was 2- to 3-fold greater in light exposed than in control retinas. Following light exposure, anti-alphaB-crystallin immunoreactivity was increased in rod outer segments and retinal pigment epithelium. These findings support a possible role for crystallins in protecting photoreceptors from light damage.
Sakaguchi
Hirokazu
H
Miyagi
Masaru
M
Darrow
Ruth M
RM
Crabb
John S
JS
Hollyfield
Joe G
JG
Organisciak
Daniel T
DT
Crabb
John W
JW
D000818:Animals; D003459:Crystallins / Q000378:metabolism*; D008027:Light / Q000009:adverse effects*; D010857:Pigment Epithelium of Eye / Q000528:radiation effects; D011832:Radiation Injuries / Q000378:metabolism*; D051381:Rats; D012160:Retina / Q000528:radiation effects*; D012374:Rod Cell Outer Segment / Q000528:radiation effects
D003459:Crystallins