Nanometer-scale pores: potential applications for analyte detection and DNA characterization.
Several classes of transmembrane protein ion channels function in vivo as sensitive and selective detection elements for analytes. Recent studies on single channels reconstituted into planar lipid bilayer membranes suggest that nanometer-scale pores can be used to detect, quantitate and characterize a wide range of analytes that includes small ions and single stranded DNA. We briefly review here these studies and identify leaps in technology that, if realized, might lead to innovations for the early detection of cancer.
Kasianowicz
John J
JJ
Biotechnology Division, National Institute of Standards and Technology, ACSL 227/A251, Gaithersburg, MD 20899-8313, USA. john.kasianowicz@nist.gov
D015374:Biosensing Techniques; D004277:DNA, Single-Stranded / Q000235:genetics*; D006801:Humans; D015640:Ion Channel Gating; D007473:Ion Channels / Q000378:metabolism; D007477:Ions; D036103:Nanotechnology
D004277:DNA, Single-Stranded; D007473:Ion Channels; D007477:Ions