title: The QDE-3 homologue RecQ-2 co-operates with QDE-3 in DNA repair in Neurospora crassa.
abstract: The post-transcriptional gene silencing mechanism in Neurospora crassa, called quelling, was shown to involve the products of three genes termed quelling-defective. A homologue to the qde-3 gene encoding a putative RecQ-type DNA helicase was isolated and was named RecQ-2. Characterisation of the RecQ-2 gene has revealed that it is not involved in quelling, but may co-operate with the qde-3 gene product in a pathway that repairs damage to DNA caused by the chemical mutagens methyl methanesulfonate and N-methyl- N'-nitro- N-nitrosoguanidine. These results indicate that the qde-3 RecQ helicase may have a dual role in N. crassa, either acting alone as an essential component of the quelling mechanism or together with the RecQ-2 RecQ helicase, as part of a process to repair DNA lesions during replication.
authors.affiliation: Istituto Pasteur, Fondazione Cenci Bolognetti, Dipartimento di Biotecnologie Cellulari e Ematologia, Università degli Studi La Sapienza, Viale Regina Elena 324, 00161 Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Pickford
authors.forename: L
authors.initials: L
authors.lastname: Braccini
authors.forename: G
authors.initials: G
authors.lastname: Macino
authors.forename: C
authors.initials: C
authors.lastname: Cogoni
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D017124:Conserved Sequence; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004260:DNA Repair / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D005656:Fungal Proteins; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D009492:Neurospora crassa / Q000235:genetics* / Q000502:physiology; D053484:RecQ Helicases
chemical_list: D005656:Fungal Proteins; D009153:Mutagens; D000251:Adenosine Triphosphatases; D004265:DNA Helicases; D053484:RecQ Helicases; C402647:QDE-3 protein, Neurospora crassa