Quantification of the copy number of nor-1, a gene of the aflatoxin biosynthetic pathway by real-time PCR, and its correlation to the cfu of Aspergillus flavus in foods.
A real-time PCR system directed against the nor-1 gene of the aflatoxin biosynthetic pathway as a target sequence has been applied to detect an aflatoxinogenic A. flavus strain in plant-type foods like maize, pepper and paprika. The system is based on the TaqMan fluorescent probe technology. The copy numbers of the nor-1 gene were compared to conventional cfu data obtained from the same set of samples. In general, a good correlation between nor-1 gene copy number and the cfu data was observed; however, the nor-1 copy numbers were always higher. It was shown that the system is specific for nor-1 containing species.
Mayer
Zsuzsanna
Z
Faculty of Food Science, Szent Itsván University, Villányi út 35-43, 1118 Budapest, Hungary.
Bagnara
Angelo
A
Färber
Paul
P
Geisen
Rolf
R
D000348:Aflatoxins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D001231:Aspergillus flavus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001483:Base Sequence; D002212:Capsicum / Q000382:microbiology; D015169:Colony Count, Microbial; D004271:DNA, Fungal / Q000032:analysis* / Q000235:genetics; D005656:Fungal Proteins; D005800:Genes, Fungal / Q000235:genetics; D064430:NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases; D016133:Polymerase Chain Reaction; D019914:Taq Polymerase; D003313:Zea mays / Q000382:microbiology
D000348:Aflatoxins; D004271:DNA, Fungal; D005656:Fungal Proteins; D000429:Alcohol Oxidoreductases; D064430:NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases; C062609:norsolorinic acid ketoreductase; D019914:Taq Polymerase