[Study on the effects of taxol on Hep-2 cell lines in larynx carcinoma].
OBJECTIVE
To investigate the sensitivity of Hep-2 cell lines in larynx squamous cell carcinoma to the taxol in vitro and to provide experimental basis for clinical therapy.


METHODS
Techniques of cell culture and randomized blank-contrast design were used. The degree and dose-dependency of taxol which induced growth inhibition in Hep-2 cell lines were observed and the alteration of cell cycle was analysed by flow cytomethy.


RESULTS
All cells died at the concentration of taxol 1 x 10(-8) M; growth inhibition was observed clearly at the concentration of 5 x 10(-9) M and 2.5 x 10(-9) M. Flow cytometric data showed a G0/G1 arrest and apoptosis (28.96%) in two cell lines after exposure to taxol for 48 h at low and medium concentrations.


CONCLUSIONS
There is a high sensitivity of Hep-2 cell to taxol in vitro. The cells show G0/G1 arrest and apoptosis. This results may provide reliable guidance for treating largnx carcinoma.
Ni
G
G
Shanghai Fourth Hospital, Shanghei 200081.
Dong
Y
Y
Wang
J
J
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D017239:Paclitaxel / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel