Human spermatozoa motility analysis in a Ringer's solution containing cupric ions.
The ability of cupric ions to immobilize human spermatozoa was assessed in vitro using an optical microscope. A Ringer's solution containing human spermatozoa and three cupric ion concentrations of 8 x 10(-8) mol/L, 8 x 10(-6) mol/L and 8 x 10(-5) mol/L were tested. The higher cupric ion concentrations (8 x 10(-5) mol/L and 8 x 10(-6) mol/L) significantly reduced spermatozoa motility after about 20 min. However, with the lower cupric ion concentration tested (8 x 10(-8) mol/L), also for 20-min experimentation, the reduction in motility was more limited.
Araya
R
R
Biochemistry Section, Institute of Chemistry, Catholic University of Valparaíso, Avda Brasil 2950, Casilla 4059, Valparaíso, Chile.
Gómez-Mora
H
H
Vera
R
R
Bastidas
J M
JM
D003300:Copper / Q000008:administration & dosage* / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007552:Isotonic Solutions / Q000008:administration & dosage* / Q000737:chemistry; D007700:Kinetics; D008297:Male; D000077331:Ringer's Solution; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects*
D007552:Isotonic Solutions; D003300:Copper; D000077331:Ringer's Solution