title: [The study on the culture, freezing preservation and resuscitation of human trabecular cells in vitro].
abstract: OBJECTIVE
  To establish the culture of human trabecular cells in vitro and study the freezing preservation and resuscitation.
  
  
  METHODS
  The trabecular specimens from human eyes were cultured in vitro and passaged 3 times, then the cultured cells were frozen and preserved. The frozen cells were resuscitated after one week, two weeks, one month and two months.
  
  
  RESULTS
  Cultured human trabecular cells were obtained. The frozen cells were resuscitated successfully. All of the resuscitated rates were more than 90%.
  
  
  CONCLUSIONS
  The human trabecular cells were cultured in vitro successfully, they can also be frozen and resuscitated, which will be helpful to the establishment of cDNA library and the screening of associated glaucomatous genes.
authors.affiliation: Zhongshan Opthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
authors.forename: J
authors.initials: J
authors.lastname: Ge
authors.forename: Y
authors.initials: Y
authors.lastname: Zhuo
authors.forename: J
authors.initials: J
authors.lastname: Zhen
authors.forename: M
authors.initials: M
authors.lastname: Lin
mesh_terms: D002455:Cell Division; D002478:Cells, Cultured; D015925:Cryopreservation; D006801:Humans; D014021:Tissue Preservation; D014129:Trabecular Meshwork / Q000166:cytology*