Mapping of the root lesion nematode ( Pratylenchus neglectus) resistance gene Rlnn1 in wheat.
The root lesion nematode, Pratylenchus neglectus, is an economically damaging pathogen of wheat and other crops. The development of P. neglectus-resistant wheat cultivars would be greatly accelerated through the use of molecular markers, as resistance phenotyping is extremely time-consuming. A greenhouse bioassay was developed to identify resistance phenotypes of doubled-haploid populations. Bulked-segregant analysis was used to identify AFLP markers linked to P. neglectus resistance in the wheat cultivar Excalibur. One resistance-linked AFLP marker was mapped close to chromosome 7A RFLP markers in a densely-mapped Cranbrook/Halberd population. One of the chromosome 7A RFLP probes, cdo347, was genotyped in the Tammin/Excalibur population segregating for response to root lesion nematode and showed 8% recombination with the P. neglectus resistance gene Rlnn1. The marker Xcdo347-7A was validated on Excalibur-and Krichauff-derived DH populations segregating for Rlnn1 and showed 14% and 10% recombination, respectively, with Rlnn1 in these populations.
Williams
J.
J
Cooperative Research Centre for Molecular Plant Breeding, South Australian Research and Development Institute, P.O. Box 397, South Australia 5001, Australia, williams.kevin@saugov.sa.gov.au
Taylor
P.
P
Bogacki
P.
P
Pallotta
M.
M
Bariana
S.
S
Wallwork
H.
H