[Isolating, cloning, and sequencing of HPV16E6E7 gene from a cervical carcinoma biopsy in Shandong Province].
OBJECTIVE
In order to study the structure specificity of HPV16E6E7 gene of a Chinese patient of cervical carcinoma in Shandong province.


METHODS
The tissue DNA were abstracted from cervical carcinoma biopsies and the type of HPV was identified by HPV multiple primers PCR. HPV16E6E7 gene was amplified by PCR from the cervical carcinoma tissue DNA with the infection of HPV16 type only, and then cloned the E6E7 gene into pALTERI vector. After sequencing the double strand, the gene was compared with the prototype E6E7 gene of HPV16.


RESULTS
A new recombinant plasmid was constructed and named HPV16E6E7-SD. Sequencing results showed one mutation in HPV16E6E7-SD, the 557th neocleotide in the viral neocleotide sequence "T" was changed into "C", and caused the termination cordon TAA of E6 gene to convert into a Gln cordon CAA.


CONCLUSIONS
There is a structure difference between HPV16E6E7-SD and the standard strain.
Xu
X
X
Department of Biophysics, Institute of Basic Medical Science, CAMS and PUMC, Beijing 100005.
Si
J
J
Liu
S
S
Xu
J
J
Liu
Z
Z
D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000821:virology; D002681:China; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005858:Germany; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000302:isolation & purification; D027383:Papillomaviridae / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D017354:Point Mutation; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology
D004273:DNA, Neoplasm; D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16