[Effect of astragalus-angelica mixture on osteopontin expression in rats with chronic nephrosclerosis].
OBJECTIVE
To observe the effect of Astragalus-Angelica Mixture (AAM) on osteopontin (OPN) expression in rats with chronic nephrosclerosis.


METHODS
Chronic nephrosclerosis model rats induced by repeated intraperitoneal injection of puromycin were randomly divided into the model group, AAM group and Irbesartan (an antagonist of angiotensin) group. The experimental course lasted 12 weeks. Blood and urine samples were examined by biochemical method. Kidney tissue was taken for pathological stain and immunohistochemical method and was applied to examine OPN expression, mononuclear macrophage, laminin in extracellular matrix and decorin expressions.


RESULTS
AAM showed the effects of decreasing urinary protein and improving renal function similar to that of Irbesartan. It also could alleviate the pathological damage of kidney tissue, especially in decreasing renal tubular mesenchymal damage index. The accumulation of decorin and laminin in the mesenchymal extracellular matrix significantly decreased. Renal tubular OPN expression and mesenchymal infiltration of mononuclear macrophage decreased significantly and in a positive correlated manner (r = 0.885, P < 0.01).


CONCLUSIONS
AAM has similar renal protective action to that of Irbesartan, this action may be related to the inhibition of up-regulated OPN expression.
Zhao
Ya-ni
YN
Renal Division of Department of Medicine, First Hospital, Peking University, Beijing 100034.
Li
Jing-zi
JZ
Yu
Ling
L
D029971:Angelica sinensis; D000818:Animals; D027883:Astragalus Plant; D027885:Astragalus propinquus; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D009400:Nephrosclerosis / Q000139:chemically induced / Q000378:metabolism*; D053495:Osteopontin; D008517:Phytotherapy; D011691:Puromycin; D051381:Rats; D017207:Rats, Sprague-Dawley; D012795:Sialoglycoproteins / Q000096:biosynthesis*
D004365:Drugs, Chinese Herbal; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin; D011691:Puromycin; C027492:Huang Qi; C044613:angelicae sinensis extract