Characterization of the human heart mitochondrial proteome.
To gain a better understanding of the critical role of mitochondria in cell function, we have compiled an extensive catalogue of the mitochondrial proteome using highly purified mitochondria from normal human heart tissue. Sucrose gradient centrifugation was employed to partially resolve protein complexes whose individual protein components were separated by one-dimensional PAGE. Total in-gel processing and subsequent detection by mass spectrometry and rigorous bioinformatic analysis yielded a total of 615 distinct protein identifications. All protein pI values, molecular weight ranges, and hydrophobicities were represented. The coverage of the known subunits of the oxidative phosphorylation machinery within the inner mitochondrial membrane was >90%. A significant proportion of identified proteins are involved in signaling, RNA, DNA, and protein synthesis, ion transport, and lipid metabolism. The biochemical roles of 19% of the identified proteins have not been defined. This database of proteins provides a comprehensive resource for the discovery of novel mitochondrial functions and pathways.
Taylor
Steven W
SW
MitoKor, 11494 Sorrento Valley Road, San Diego, California 92121, USA. taylors@mitokor.com
Fahy
Eoin
E
Zhang
Bing
B
Glenn
Gary M
GM
Warnock
Dale E
DE
Wiley
Sandra
S
Murphy
Anne N
AN
Gaucher
Sara P
SP
Capaldi
Roderick A
RA
Gibson
Bradford W
BW
Ghosh
Soumitra S
SS
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D030562:Databases, Protein; D004586:Electrophoresis / Q000379:methods; D006321:Heart / Q000502:physiology; D006801:Humans; D016247:Information Storage and Retrieval / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D008928:Mitochondria / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D024101:Mitochondrial Proteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000502:physiology*; D008970:Molecular Weight; D009206:Myocardium / Q000737:chemistry; D020543:Proteome / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D040901:Proteomics / Q000379:methods; D020539:Sequence Analysis, Protein / Q000379:methods
D024101:Mitochondrial Proteins; D020543:Proteome