[Identification of epitope of 22.6 kDa antigen of Schistosoma japonicum with phage-displayed random peptide library].
OBJECTIVE
To identify the epitopes of 22.6 kDa antigen of Schistosoma japonicum (Sj22.6).


METHODS
A 12-mer library displayed on pIII of fd phage was used to screen the epitopes of Sj22.6 with the purified multiple clone IgG antibody against the Sj22.6 antigen. Five rounds of biopanning were carried out and fourteen clones from the fifth round biopanning were randomly selected and identified by Western blotting. The mice were immunized with the obtained positive clones and the antibody titers of sera from the mice were detected. The clones which could stimulate the mice to produce anti-Sj22.6 antibodies were sequenced and their amino acid sequences were compared with that of Sj22.6.


RESULTS
Six clones selected from the fourteen ones could stimulate the mice to produce anti-Sj22.6 antibodies analysed by Western blotting. The amino acid sequence of one epitope showed high homology with Sj22.6.


CONCLUSIONS
Four epitopes of Sj22.6 were obtained. One may be a structure epitope and the other three were mimic epitopes.
Hu
Xue-mei
XM
Department of Immunology, Binzhou Medical College, Binzhou 256603.
Zhang
Zhao-song
ZS
Wu
Hai-wei
HW
Su
Chuan
C
Ji
Min-jun
MJ
Wang
Yong
Y
Chen
Shu-zhen
SZ
Wu
Guan-ling
GL
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology*; D001483:Base Sequence; D000939:Epitopes / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D019151:Peptide Library; D012549:Schistosoma japonicum / Q000276:immunology*
C087430:22.6-kDa antigen, Schistosoma japonicum; D000947:Antigens, Helminth; D000939:Epitopes; D008565:Membrane Proteins; D019151:Peptide Library