Quantitative study of basic fibroblast growth factor in vitreous with proliferative vitreoretinopathy.
OBJECTIVE
To quantitatively study basic fibroblast growth factor (bFGF) in the vitreous of proliferative vitreoretinopathy (PVR) in order to understand the role of bFGF in the development of PVR.


METHODS
High sensitive sandwich enzyme immunoassay technique (ELISA) was used to measure bFGF level in vitreous of normal eyes, the eyes of PVR-C or PVR-D grade, eyes of vitreous hemorrhage and the serum levels of bFGF in PVR-D patients.


RESULTS
The levels of bFGF in the vitreous were: median 5.20 ng/L, quartile 15.47 ng/L in 20 normal eyes; median 3.12 ng/L, quartile 10.48 ng/L in 35 PVR-C eyes; median 46.56 ng/L, quartile 113.96 ng/L in 26 PVR-D eyes; median 1.40 ng/L, quartile 6.25 ng/L in 25 vitreous hemorrhage eyes. The vitreous bFGF level in PVR-D group was significantly higher than that in the normal group, PVR-C group and vitreous hemorrhage group (P < 0.01). The mean of serum-bFGF level was 18.33 +/- 3.39 ng/L. The vitreous bFGF level of PVR-D group was significantly higher than serum-bFGF level (P < 0.01). And the vitreous-bFGF level in PVR-D group was significantly higher in larger retinal tear subgroup.


CONCLUSIONS
The results suggested that bFGF is involved in the development of PVR.
Liang
X
X
Zhongshan Ophthalmology Genter, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Li
C
C
Gao
R
R
Ding
X
X
Tang
S
S
Xie
C
C
Li
S
S
D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012167:Retinal Perforations / Q000378:metabolism; D018630:Vitreoretinopathy, Proliferative / Q000378:metabolism*; D014822:Vitreous Body / Q000737:chemistry*
D016222:Fibroblast Growth Factor 2