Chemiluminescence flow-through sensor for the determination of vitamin B2 using controlled-reagent-release technology.
OBJECTIVE
To establish a simple and novel method for the determination of vitamin B2 rapidly in pharmaceutical preparations.


METHODS
Vitamin B2 was determined by a chemiluminescence (CL) sensor combined with flow-injection (FI) technology. The analytical reagents involved in the CL reaction, luminol and hexacyanoferrate (III), were both immobilized on an anion-exchange resin column in FI system. The CL signal produced by the reaction between luminol and hexacyanoferrate (III), which were eluted from the column through sodium phosphate injection, decreased in the presence of vitamin B2.


RESULTS
The decreased CL intensity was linearly correlated with the vitamin B2 concentration in the range of 0.01-1.0 microgram.mL-1, the detection limit was 4.0 ng.mL-1 vitamin B2 (3 sigma). At a flow rate of 2.0 mL.min-1, the procedure including sampling and washing could be performed in 2 min with a relative standard deviation of less than 3.0%.


CONCLUSIONS
The flow sensor exhibited both good sensitivity and stability. It could be reused more than 450 times and has been applied successfully to the analysis of vitamin B2 in pharmaceutical preparations.
Wang
Lin
L
Department of Chemistry, Northwest University, Xi'an 710069, China.
Song
Zheng-hua
ZH
D017022:Flow Injection Analysis / Q000379:methods*; D008163:Luminescent Measurements; D008165:Luminol; D012256:Riboflavin / Q000032:analysis*; D013607:Tablets / Q000737:chemistry
D013607:Tablets; D008165:Luminol; D012256:Riboflavin