title: Phenotypic analysis of hepatic lymphocytes from healthy dogs.
abstract: Phenotypes of lymphocytes from laparoscopically biopsied liver tissues of eleven healthy beagle dogs were analyzed. The proportion of CD3(+) lymphocytes (T cells), CD3 (-)CD21(+) lymphocytes (B cells) and CD3 (-)CD21(-) lymphocytes (non-T non-B lymphocytes), and the CD4(+)/CD8(+) ratio in the canine hepatic lymphocytes were 54.8 +/- 11.9%, 4.7 +/- 3.1%, 40.7 +/- 13.2%, and 0.33 +/- 0.12, respectively, while those in peripheral blood lymphocytes were 85.4 +/- 6.5%, 9.3 +/- 6.1%, 5.3 +/- 1.8%, and 1.64 +/- 0.36, respectively. These results indicated that the constitution of hepatic lymphocytes quite differed from that of peripheral blood lymphocytes in dogs, and suggested that the regional immunity in canine liver might be specific.
authors.affiliation: Laboratory of Comprehensive Veterinary Clinical Studies, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Sakai
authors.forename: Isao
authors.initials: I
authors.lastname: Otani
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Watari
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Sato
authors.forename: Kiichi
authors.initials: K
authors.lastname: Kanayama
authors.forename: Akira
authors.initials: A
authors.lastname: Takeuchi
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hasegawa
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D002470:Cell Survival; D004285:Dogs / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D006262:Health; D016130:Immunophenotyping / Q000662:veterinary; D008099:Liver / Q000166:cytology* / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000166:cytology*; D008297:Male
chemical_list: D015703:Antigens, CD