title: [Inhibitory effects of isorhapotigenin on IL-8 production and mRNA expression induced with TNF alpha in normal human synovial cells].
abstract: OBJECTIVE
  To study the effects of isorhapotigenin (Iso) on interleukin-8 (IL-8) production and mRNA expression in normal human synovial cells (HSC) induced with TNF alpha.
  
  
  METHODS
  IL-8 were assayed with RIA method. The mRNA expression of IL-8 was detected by RT-PCR method.
  
  
  RESULTS
  It was shown that TNF alpha at concentrations of 0.05 to 0.5 U.mL-1 for 24 h significantly increased IL-8 production. The expression of IL-8 mRNA was also promoted by TNF alpha (0.25 U.mL-1) for 6 h. Iso at the concentrations of 1 x 10(-6) mol.L-1 to 1 x 10(-5) mol.L-1 showed inhibitory effects on IL-8 production induced with TNF alpha (0.25 U.mL-1). The further study indicated that Iso at the concentrations of 1 x 10(-6) mol.L-1 to 1 x 10(-5) mol.L-1 inhibited IL-8 mRNA expression in HSC induced with TNF alpha (0.25 U.mL-1).
  
  
  CONCLUSIONS
  TNF alpha promoted IL-8 production and mRNA expression in HSC. Iso inhibited IL-8 production and mRNA expression induced by TNF alpha (0.25 U.mL-1). This might be one of the anti-inflammatory mechanisms of Iso.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Hou
authors.forename: L C
authors.initials: LC
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Guo
authors.forename: G F
authors.initials: GF
authors.lastname: Cheng
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D004347:Drug Interactions; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000494:pharmacology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016209:Interleukin-8; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha