Small, acid-soluble proteins as biomarkers in mass spectrometry analysis of Bacillus spores.
The use of 1 N HCl for extraction of small, acid-soluble proteins (SASP) from different Bacillus spore species was examined. The extracts were analyzed by high-performance liquid chromatography and matrix-assisted laser desorption mass spectrometry and were found to be both qualitatively and quantitatively superior to extraction by acetonitrile-5% trifluoroacetic acid (70:30, vol/vol). Both major and minor alpha/beta- and gamma-type SASP were characterized by their molecular masses or tryptic peptide maps and by searches of both protein and unannotated genome databases. For all but 1 pair (B. cereus T and B. thuringiensis subsp. Kurstaki) among the 11 variants studied the suites of SASP masses are distinctive, consistent with the use of these proteins as potential biomarkers for spore identification by mass spectrometry.
Hathout
Yetrib
Y
Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20704, USA. hathout@wam.umd.edu
Setlow
Barbara
B
Cabrera-Martinez
Rosa-Martha
RM
Fenselau
Catherine
C
Setlow
Peter
P
D000595:Amino Acid Sequence; D001407:Bacillus / Q000737:chemistry* / Q000145:classification / Q000502:physiology*; D001426:Bacterial Proteins / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis*; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013171:Spores, Bacterial / Q000737:chemistry / Q000145:classification / Q000502:physiology
D001426:Bacterial Proteins; D015415:Biomarkers