title: Expression of active human blood clotting factor VIII in mammary gland of transgenic rabbits.
abstract: Human clotting factor VIII is probably the largest protein to be expressed to date in the mammary gland of a transgenic animal, and it requires extensive posttranslational modification to achieve full biological activity. The mammary gland specific construct mWAP-hFVIII-MT-I was injected into the pronuclei of rabbit zygotes, and three transgenic offspring were obtained. Founder 385 showed germ-line transmission of a single integrated copy, and a homozygous line was established from this animal. The rhFVIII was transcribed and translated exclusively in the mammary gland. The activity of rhFVIII in the rabbit milk ranged from 5 to 8% of that found in normal human plasma. Results indicate the suitability of the transgenic rabbit mammary gland for rhFVIII production.
authors.affiliation: Institute of Animal Biology, Agricultural Biotechnology Center, Gödöllö, Hungary.
authors.forename: L
authors.initials: L
authors.lastname: Hiripi
authors.forename: F
authors.initials: F
authors.lastname: Makovics
authors.forename: R
authors.initials: R
authors.lastname: Halter
authors.forename: M
authors.initials: M
authors.lastname: Baranyi
authors.forename: D
authors.initials: D
authors.lastname: Paul
authors.forename: J W
authors.initials: JW
authors.lastname: Carnwath
authors.forename: Zs
authors.initials: Z
authors.lastname: Bösze
authors.forename: H
authors.initials: H
authors.lastname: Niemann
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000378:metabolism*; D001777:Blood Coagulation / Q000502:physiology; D005169:Factor VIII / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006801:Humans; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008892:Milk / Q000378:metabolism*; D011817:Rabbits
chemical_list: D005169:Factor VIII