Molecular monitoring in malaria vaccine trials.
Molecular techniques offer new approaches for malaria field trials, particularly PCR techniques, which facilitate accurate diagnosis of Plasmodium infections and increase the power of estimates of vaccine effects on malaria prevalence or incidence. Molecular methods also help to assess selective effects of vaccines. Longitudinal genotyping data can be used to initiate novel analyses of parasite dynamics, including estimates of incidence of infection with individual parasite clones and duration of infections. In addition, high-throughput methods can be used to apply these techniques routinely in randomized controlled trials, as well as programme-based evaluations of malaria control.
Felger
Ingrid
I
Swiss Tropical Institute, Socinstrasse 57, PO Box 4002, Basel, Switzerland. ingrid.felger@unibas.ch
Genton
Blaise
B
Smith
Tom
T
Tanner
Marcel
M
Beck
Hans Peter
HP
D000818:Animals; D002986:Clinical Trials as Topic; D005838:Genotype; D006801:Humans; D008288:Malaria / Q000175:diagnosis* / Q000517:prevention & control; D017780:Malaria Vaccines; D016778:Malaria, Falciparum / Q000175:diagnosis / Q000517:prevention & control; D010961:Plasmodium / Q000235:genetics* / Q000276:immunology; D010963:Plasmodium falciparum / Q000235:genetics / Q000276:immunology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D016896:Treatment Outcome
D017780:Malaria Vaccines