Enzyme catalysis in microgravity: steady-state kinetic analysis of the isocitrate lyase reaction.
Two decades of research in microgravity have shown that certain biochemical processes can be altered by weightlessness. Approximately 10 years ago, our team, supported by the European Space Agency (ESA) and the Agenzia Spaziale Italiana, started the Effect of Microgravity on Enzyme Catalysis project to test the possibility that the microgravity effect observed at cellular level could be mediated by enzyme reactions. An experiment to study the cleavage reaction catalyzed by isocitrate lyase was flown on the sounding rocket MASER 7, and we found that the kinetic parameters were not altered by microgravity. During the 28th ESA parabolic flight campaign, we had the opportunity to replicate the MASER 7 experiment and to perform a complete steady-state analysis of the isocitrate lyase reaction. This study showed that both in microgravity and in standard g controls the enzyme reaction obeyed the same kinetic mechanism and none of the kinetic parameters, nor the equilibrium constant of the overall reaction were altered. Our results contrast with those of a similar experiment, which was performed during the same parabolic flight campaign, and showed that microgravity increased the affinity of lipoxygenase-1 for linoleic acid. The hypotheses suggested to explain this change effect of the latter were here tested by computer simulation, and appeared to be inconsistent with the experimental outcome.
Ranaldi
Francesco
F
Department of Biochemical Sciences, University of Florence, viale Pieraccini, 6, I-50139 Florence, Italy.
Vanni
Paolo
P
Giachetti
Eugenio
E
D002384:Catalysis; D004798:Enzymes / Q000378:metabolism; D018472:Hypogravity; D007522:Isocitrate Lyase / Q000378:metabolism*; D007700:Kinetics; D008962:Models, Theoretical; D028223:Pinus / Q000201:enzymology; D013026:Space Flight; D014893:Weightlessness
D004798:Enzymes; D007522:Isocitrate Lyase