[Analysis of tannins in Fructus Chebulae and its confusion varieties by HPCE].
OBJECTIVE
To analyze the hydrolyzable tannins-chebulinic acid (I) and chebulagic acid(II) in Fructus Chebulae and its confusion varieties by using high performance capillary electrophoresis (HPCE) method.


METHODS
Using a capillary (375 microns OD x 50 microns ID; 81.5 cm x 61.5 cm) and a power supply set at 24 kV, with phosphate-borate buffer containing 20 mmol.L-1 Na2HPO4-60 mmol.L-1 boric acid and a UV detector at 280 nm, sample solution was loaded in decompression mode at the positive end of the capillary, the loading time was 5 s.


RESULTS
The linear ranges of I and II were 0.0842-0.842 and 0.842 and 0.0940-0.940 mg.mL-1 respectively, the correlation coefficient were 0.9966 and 0.9957, the average recoveries were 95.6% (RSD = 4.0%, n = 5) and 95.0% (RSD = 4.4%, n = 5), the RSDs (n = 5) of measurement precision test were 2.2% and 1.7%, the RSDs (n = 6) of reproduction test were 5.4% and 4.0% respectively. The contents of I and II were obviously interrelated with the variety and characteristics of Fructus Chebulae, the contents of I and II in the confusion varieties of Fructus Chebulae were very low.


CONCLUSIONS
It is suitable to use I and II as the criterion in quality evaluation of Fructus Chebulae, and the HPCE method is effective for quality evaluation of the crude Fructus Chebulae.
Ding
G
G
Department of Phytochemistry, Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China. dingg2000@china.com
Lu
Y R
YR
Ji
C R
CR
Liu
Y Z
YZ
D001578:Benzopyrans / Q000032:analysis*; D004340:Drug Contamination; D019075:Electrophoresis, Capillary; D005638:Fruit / Q000737:chemistry; D005960:Glucosides / Q000032:analysis*; D047348:Hydrolyzable Tannins; D010946:Plants, Medicinal / Q000737:chemistry* / Q000145:classification; D011786:Quality Control; D013634:Tannins / Q000032:analysis*; D029766:Terminalia / Q000737:chemistry* / Q000145:classification
D001578:Benzopyrans; D005960:Glucosides; D047348:Hydrolyzable Tannins; D013634:Tannins; C103481:chebulinic acid; C076178:chebulagic acid