[Autocrine regulation for transforming growth factor-beta 1 by fibroblast in vitro].
OBJECTIVE
To explore the autocrine regulation for the transforming growth factor-beta 1(TGF-beta 1) by the fibroblasts in vitro.


METHODS
Fibroblasts were cultured in vitro which isolated from the normal prepuce by circumcision. TGF-beta 1 concentration were determined with ELISA for the different passages and the different time at the same passage.


RESULTS
The TGF-beta 1 concentration was different for the distinct passage of fibroblasts and achieved the peak (450 ng/L) at the sixth passage. Among the same fibroblasts, the TGF-beta 1 concentration was different in the various days and passage of the summit (680 ng/L) on the fifth day, which was 2.5 times as high as that of the first day.


CONCLUSIONS
The fibroblasts possess the ability of autocrine of the TGF-beta 1 and have some regularity. It will provide the theory basis for the research about TGF-beta 1 regulation mechanism and the medical application about salvia miltiorrhiza.
Zhang
Jie
J
Institute of Cancer, West China Hospital, Sichuan University, Chengdu, Sichuan, P. R. China 610041.
Lin
Ping
P
Huang
Lu-gang
LG
D002455:Cell Division; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*; D053773:Transforming Growth Factor beta1
C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1