Separation of andrographolide and neoandrographolide from the leaves of Andrographis paniculata using high-speed counter-current chromatography.
The bioactive diterpenes andrographolide and neoandrographolide from the leaves of Andrographis paniculata NEES (Acanthaceae) were successfully separated by counter-current chromatography. A single 280-min separation yielded 189 mg of 99.9% andrographolide and 9.5 mg of 98.5% neoandrographolide applying water-methanol-ethyl acetate-n-hexane (2.5:2.5:4:1) solvent system. Structure confirmation was done by electrospray MS, one-dimensional NMR experiments, circular dichroism, optical rotation dispersion, and specific optical rotation [alpha]D.
Qizhen
Du
D
Institute of Food and Biological Engineering, Hangzhou University of Commerce, Hangzhou 310035, China. qizhendu@hotmail.com
Jerz
Gerold
G
Winterhalter
Peter
P
D035685:Andrographis / Q000737:chemistry*; D002942:Circular Dichroism; D003377:Countercurrent Distribution / Q000379:methods*; D004224:Diterpenes / Q000302:isolation & purification*; D005960:Glucosides / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D018515:Plant Leaves / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013764:Tetrahydronaphthalenes / Q000302:isolation & purification*
D004224:Diterpenes; D005960:Glucosides; D013764:Tetrahydronaphthalenes; C030419:andrographolide; C082497:neoandrographolide