[Recombination and cloning of MSP1(19) and PfCMR of Plasmodium falciparum].
OBJECTIVE
To construct a recombinant plasmid DNA encoding multiantigens of Plasmodium falciparum and to provide the requirements for DNA immunization.


METHODS
Two oligonucleotide primers were designed to amplify MSP1(19), the purified PCR products were digested by Sal I + Xba I, and the recombinant plasmid pWR450-1/PfCMR was digested by EcoR I + Sal I to recover PfCMR gene. PfCMR and MSP1(19) gene fragments were linked and recombined with mammalian expression vector pcDNA3.


RESULTS
The MSP1(19) gene fragment with about 363 base pairs were specifically amplified by using PCR technique. The positive recombinant pcDNA3-PfCMR-MSP1(19) (named pcDNA3-Pf8) was screened and identified by agarose gel electrophoresis, endonulease digestion and PCR technique, the whole length of Pf8 is 618 bp.


CONCLUSIONS
By specifically amplifying MSP1(19) gene at the C-terminal of MSP1, a recombinant plasmid pcDNA3-Pf8 encoding multiantigens of Plasmodium falciparum was successfully constructed.
Li
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Department of Parasitology, Sun Yat-sen University of Medical Sciences, Guangzhou 510089.
Yu
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X
Luo
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D000818:Animals; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D026901:Membrane Transport Proteins; D020066:Merozoite Surface Protein 1 / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins; D019444:Vaccines, DNA
D004274:DNA, Recombinant; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D020066:Merozoite Surface Protein 1; C416369:PfCRT protein, Plasmodium falciparum; D015800:Protozoan Proteins; D019444:Vaccines, DNA