Differential roles of the two-component peptides of lactocin 705 in antimicrobial activity.
Lactobacillus casei CRL705 produces a class IIb bacteriocin, lactocin 705, which relies on the complementary action of two components, Lac705alpha and Lac705beta. These peptides exert a bactericidal effect on the indicator strain Lactobacillus plantarum CRL691, with an optimal Lac705alpha/Lac705beta peptide ratio of 1 to 4. Electron microscopy studies showed that treated CRL691 cells have their cell wall severely damaged, with mesosome-like membranous formations protruding into their cytoplasm. Although less pronounced, a similar effect was also observed with the Lac705beta peptide alone. Furthermore, Lac705beta increased the inhibitory action of a diluted supernatant of L. casei CRL705, while Lac705alpha protected CRL691 cells from inhibition. Both peptides were required to dissipate the proton motive force (Deltapsi and DeltapH) of CRL691 cells. These data suggested that of the two components of lactocin 705, the Lac705alpha peptide is responsible for receptor recognition, and the Lac705beta peptide is the active component on the cell membrane of CRL691 cells.
Cuozzo
Sergio A
SA
Centro de Referencia para Lactobacilos (CERELA), CONICET, Chacabuco 145, 4000 Tucumán, Argentina.
Castellano
Patricia
P
Sesma
Fernando J M
FJ
Vignolo
Graciela M
GM
Raya
Raul R
RR
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D001430:Bacteriocins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D007780:Lacticaseibacillus casei / Q000235:genetics / Q000378:metabolism; D010455:Peptides; D017433:Protein Structure, Secondary
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010455:Peptides; C417164:plantaricin UG1; C035728:lactocin