title: In vitro antimalarial effect of daphnetin relating to its iron-chelating activity.
abstract: OBJECTIVE
  To investigate the in vitro antimalarial effect of daphentin relating to its iron-chelating activity.
  
  
  METHODS
  Schizontocidal activity of daphnetin and desferrioxamine B was tested through an in vitro assay based on the routine in vitro cultivation of Plasmodium faciparum FCC1 strain. The iron-chelating ability of each was measured by the fluorescent probe calcein.
  
  
  RESULTS
  Daphnetin exhibited a modest iron-chelating ability compared with the powerful iron-chelator desferrioxamine B. In vitro test at the range of 0-12 mumol/L daphnetin showed a dose-dependent schizontocidal activity which could be inhibited if mixed with Fe2+ in a ratio of 2:1.
  
  
  CONCLUSIONS
  The dose-dependent antimalarial activity of daphnetin is related to its iron-chelating activity.
authors.affiliation: Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
authors.forename: Ling-yun
authors.initials: LY
authors.lastname: Mu
authors.forename: Qin-mei
authors.initials: QM
authors.lastname: Wang
authors.forename: Yi-chang
authors.initials: YC
authors.lastname: Ni
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000378:metabolism / Q000494:pharmacology*; D003676:Deferoxamine / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007502:Iron Chelating Agents / Q000378:metabolism / Q000494:pharmacology*; D010963:Plasmodium falciparum / Q000187:drug effects*; D014468:Umbelliferones / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000962:Antimalarials; D007502:Iron Chelating Agents; D014468:Umbelliferones; D003676:Deferoxamine; C039952:daphnetin