Systematic discovery of new genes in the Saccharomyces cerevisiae genome.
We used genome-wide comparative analysis of predicted protein sequences to identify many novel small genes, named smORFs for small open reading frames, within the budding yeast genome. Further analysis of 117 of these new genes showed that 84 are transcribed. We extended our analysis of one smORF conserved from yeast to human. This investigation provides an updated and comprehensive annotation of the yeast genome, validates additional concepts in the study of genomes in silico, and increases the expected numbers of coding sequences in a genome with the corresponding impact on future functional genomics and proteomics studies.
Kessler
Marco M
MM
Genome Therapeutics Corporation, Waltham, Massachusetts 02453, USA.
Zeng
Qiandong
Q
Hogan
Sarah
S
Cook
Robin
R
Morales
Arturo J
AJ
Cottarel
Guillaume
G
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D002417:Cattle; D002874:Chromosome Mapping / Q000379:methods; D015825:Chromosomes, Fungal / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D016681:Genome, Fungal; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011506:Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D029742:Caenorhabditis elegans Proteins; D029721:Drosophila Proteins; D011506:Proteins