title: [Construction of a pufferfish gridded cosmid library].
abstract: OBJECTIVE
  Pufferfish is a newly-established model organism in human genome research, which has been demonstrated its unique advantages in comparative genomics. Construction of a genomic library of pufferfish is the prerequisite to any studies of its genome.
  
  
  METHODS
  Genomic DNA from Fugu rubripes, a species distribute only in Japan and China seas, was used to construct a library in a novel cosmid vector, named sCOGH2, which contains an exon-trapping cassette.
  
  
  RESULTS
  This library is composed of 57,600 clones gridded in 60,096-well cell culture plates, one clone each well. The average size of the inserts is around 35 kb. It provides 99.2% probability to find any unique DNA fragment of pufferfish in this library. The clones of this library can survive after more than 10 times freeze-thaw, also demonstrating high stability in propagation. The clones shone highly positive signals when hybridized with the pufferfish genomic DNA.
  
  
  CONCLUSIONS
  The library accords with qualitative demands of a cosmid library.
authors.affiliation: Department of Medical Genetics, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: W
authors.initials: W
authors.lastname: Zhou
authors.forename: H
authors.initials: H
authors.lastname: Yang
mesh_terms: D000818:Animals; D003360:Cosmids / Q000235:genetics* / Q000302:isolation & purification; D015698:Genomic Library; D023741:Takifugu / Q000235:genetics*