Specific detection of Arcobacter and Campylobacter strains in water and sewage by PCR and fluorescent in situ hybridization.
The aim of this study was to evaluate PCR and fluorescent in situ hybridization (FISH) techniques for detecting Arcobacter and Campylobacter strains in river water and wastewater samples. Both 16S and 23S rRNA sequence data were used to design specific primers and oligonucleotide probes for PCR and FISH analyses, respectively. In order to assess the suitability of the methods, the assays were performed on naturally and artificially contaminated samples and compared with the isolation of cells on selective media. The detection range of PCR and FISH assays varied between 1 cell/ml (after enrichment) to 10(3) cells/ml (without enrichment). According to our results, both rRNA-based techniques have the potential to be used as quick and sensitive methods for detection of campylobacters in environmental samples.
Moreno
Yolanda
Y
Departamento de Biotecnología, Universidad Politécnica, 46022 Valencia, Spain.
Botella
Salut
S
Alonso
José Luis
JL
Ferrús
María A
MA
Hernández
Manuel
M
Hernández
Javier
J
D020640:Arcobacter / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D002167:Campylobacter / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*
D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D012722:Sewage