[Establishment and identification of the monoepitopic monoclonal antibody of Plasmodium falciparum].
OBJECTIVE
To prepare a monoepitopic monclonal antibody of Plasmoduim falciparum.


METHODS
According to the theory of protein structure, a peptide with 6-9 residues representting the antigenicity of the original histidine-rich protein II (HRP-II) of P. falciparum was synthesized and used to immunize BALB/c mice after purity identification by capillary electrophoresis.


RESULTS
Four hybridoma cell lines targeting a suitable peptide from pf HRP-II were obtained by using spleen embedment method and hybridoma technology.


CONCLUSIONS
It is the first report to prepare a monoepitopic monoclonal antibody against original protein by selecting a suitable peptide from the primary sequence of protein.
Qu
J
J
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Yang
B
B
Bao
Y
Y
Yang
Y
Y
Xu
Y
Y
Xu
X
X
Fan
P
P
Ren
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002460:Cell Line; D006825:Hybridomas / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010963:Plasmodium falciparum / Q000276:immunology*; D011506:Proteins / Q000138:chemical synthesis / Q000276:immunology*; D000099053:Histidine-Rich Glycoprotein
D000911:Antibodies, Monoclonal; D011506:Proteins; D000099053:Histidine-Rich Glycoprotein