title: Latrunculin B or ATP depletion induces cofilin-dependent translocation of actin into nuclei of mast cells.
abstract: Increasing cellular G-actin, using latrunculin B, in either intact or permeabilized rat peritoneal mast cells, caused translocation of both actin and an actin regulatory protein, cofilin, into the nuclei. The effect was not associated with an increase in the proportion of apoptotic cells. The major part of the nuclear actin was not stained by rhodamine-phalloidin but could be visualized with an actin antibody, indicating its monomeric or a conformationally distinct state, e.g. cofilin-decorated filaments. Introduction of anti-cofilin into permeabilized cells inhibited nuclear actin accumulation, implying that an active, cofilin-dependent, import exists in this system. Nuclear actin was localized outside the ethidium bromide-stained region, in the extrachromosomal nuclear domain. In permeabilized cells, the appearance of nuclear actin and cofilin was not significantly affected by increasing [Ca(2+)] and/or adding guanosine 5'-O-(3-thiotriphosphate), but was greatly promoted when ATP was withdrawn. Similarly, ATP depletion in intact cells also induced nuclear actin accumulation. In contrast to the effects of latrunculin B, ATP depletion was associated with an increase in cortical F-actin. Our results suggest that the presence of actin in the nucleus may be required for certain stress-induced responses and that cofilin is essential for the nuclear import of actin.
authors.affiliation: Physiology Department, University College London, University Street, United Kingdom.
authors.forename: Annmarie
authors.initials: A
authors.lastname: Pendleton
authors.forename: Brian
authors.initials: B
authors.lastname: Pope
authors.forename: Alan
authors.initials: A
authors.lastname: Weeds
authors.forename: Anna
authors.initials: A
authors.lastname: Koffer
mesh_terms: D051339:Actin Depolymerizing Factors; D000199:Actins / Q000378:metabolism*; D021581:Active Transport, Cell Nucleus; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000494:pharmacology; D008387:Marine Toxins / Q000494:pharmacology; D008407:Mast Cells / Q000378:metabolism*; D008840:Microfilament Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D010590:Phalloidine / Q000494:pharmacology; D011487:Protein Conformation; D051381:Rats; D012235:Rhodamines / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology*; D053778:Thiazolidines
chemical_list: D051339:Actin Depolymerizing Factors; D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D005456:Fluorescent Dyes; D008387:Marine Toxins; D008840:Microfilament Proteins; D012235:Rhodamines; D013844:Thiazoles; D053778:Thiazolidines; D010590:Phalloidine; D000255:Adenosine Triphosphate; C037068:latrunculin B; D002118:Calcium