Utilization of a particle gun DNA introduction system for the analysis of cis-regulatory elements controlling the spatial expression pattern of the arylsulfatase gene (HpArs) in sea urchin embryos.
We applied a particle gun method to introduce DNA into fertilized sea urchin eggs for the analysis of cis-regulatory elements responsible for spatial gene expression during development. We introduced HpArs (sea urchin arylsulfatase gene) -GFP and HpArs-LacZfusion constructs into the fertilized eggs and obtained high expression levels of the fusion genes. Using this assay system, we demonstrated that a fragment of HpArs (-3,484 to +4,636) is sufficient for aboral ectoderm-specific expression, and that the region in the first intron from +406 to +1,993 contains the control elements responsible for the repression of the HpArs promoter activity in secondary mesenchyme cells.
Kurita
Manabu
M
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739-8526, Japan.
Kondoh
Hitoshi
H
Mitsunaga-Nakatsubo
Keiko
K
Shimotori
Taishin
T
Sakamoto
Naoaki
N
Yamamoto
Takashi
T
Shimada
Hiraku
H
Takata
Kazuko
K
Akasaka
Koji
K
D000818:Animals; D019677:Artificial Gene Fusion; D001192:Arylsulfatases / Q000235:genetics*; D019470:Biolistics; D002874:Chromosome Mapping; D005697:Galactosides; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D007211:Indoles; D012617:Sea Urchins / Q000196:embryology* / Q000235:genetics*; D013194:Staining and Labeling
D005697:Galactosides; D007211:Indoles; D001192:Arylsulfatases; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside