Extraction of Chlamydia pneumoniae DNA from vascular tissue for use in PCR: an evaluation of four procedures.
The objective of this study was to compare four procedures for Chlamydia pneumoniae DNA extraction from vascular tissue. The NucliSens Kit, the QIAamp tissue DNA MiniKit, buffer-saturated phenol and the Geneclean II Kit were evaluated, based on the yield of recovered DNA, using PCR to detect C. pneumoniae in vascular tissue. The QIAamp tissue procedure had the highest detection level (0.004 inclusion-forming units/sample). All methods, except NucliSens (70 min), had a short handling time (30-40 min). Costs varied from 0.5 to 3.2 Euro.
Berg
H F
HF
Department of Medical Microbiology, St Elisabeth Hospital, PO Box 747, 5000 AS Tilburg, the Netherlands.
Maraha
B
B
Bergmans
A M C
AM
van der Zee
A
A
Kluytmans
J A J W
JA
Peeters
M F
MF
D002690:Chlamydia Infections / Q000473:pathology*; D016993:Chlamydophila pneumoniae / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*
D004269:DNA, Bacterial