Surface functionalization of porous polypropylene membranes with polyaniline for protein immobilization.
Commercial porous polypropylene membranes were chemically modified with polyaniline (PANI) using ammonium persulfate as the oxidizer. The influence of polymerization conditions on the membrane properties was studied by adsorption analysis and membrane permeability. The PANI-coated polypropylene (PANI/PP) membranes possessed high affinity toward the proteins, which can be immobilized onto the membrane surface through physical adsorption or covalent immobilization. The quantity of immobilized horseradish peroxidase (HRP) and its activity depended on the quantity and quality (oxidation level) of PANI. The storage conditions for PANI/PP membranes containing immobilized HRP were studied. HRP immobilized on the PANI/PP membrane was shown to retain 70% of its activity after 3-month storage at +5 degrees C, suggesting that this material can be used for practical application, such as in bioreactors as enzyme membranes.
Piletsky
Sergey
S
Institute of BioScience and Technology, Cranfield University at Silsoe, Bedfordshire, MK45 4DT, United Kingdom. s.piletsky@cranfield.ac.uk
Piletska
Elena
E
Bossi
Alessandra
A
Turner
Nicholas
N
Turner
Anthony
A
D000327:Adsorption; D000814:Aniline Compounds / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D004356:Drug Storage; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry*; D008567:Membranes, Artificial; D010539:Permeability; D011126:Polypropylenes / Q000737:chemistry*; D016062:Porosity; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry; D013499:Surface Properties
D000814:Aniline Compounds; D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D011126:Polypropylenes; D011506:Proteins; C416807:polyaniline; D006735:Horseradish Peroxidase