[The effect of cAMP on the transcription and regulation of angiotensin II type 1 receptor gene in cardiomyocytes of neonatal rats].
OBJECTIVE
To clarify the effect of cAMP on the transcription of angiotensin II type 1 (AT1) receptor mRNA in cardiomyocytes.


METHODS
Cardiomyocytes from SD neonatal rats were cultured in DMEM containing 10% fetal bovine serum (FBS) with 0.1 mmol/L 5-bromodeoxyuridine (Brdu). At day 5-7, primary cultured cells were used. FBS and Brdu were deprived 24 h before the stimulation. The levels of AT1 mRNA in primary cultured cardiomyocytes were examined by RT-PCR.


RESULTS
Stimulation of forskolin (30 mumol/L) plus 3-isobutyl-1-methyl-xanthine (IBMX, 30 mumol/L) which induced the accumulation of cellular cAMP decreased the expression of AT1 receptor mRNA to (64.4 +/- 20.3)% of control at 6 h treatment; while it increased the expression of AT1 receptor mRNA to (209.6 +/- 24.0)% of control at 48 h treatment.


CONCLUSIONS
cAMP regulated the transcription of AT1 receptor gene.
Cui
Z
Z
Department of Biochemistry, Fu Wai Hospital, CAMS and PUMC, Beijing 100037.
Chang
W
W
Chen
X
X
Chen
L
L
Liu
L
L
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology*; D005260:Female; D008297:Male; D032383:Myocytes, Cardiac / Q000166:cytology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin; D000242:Cyclic AMP