title: Fast determination of catechins and xanthines in tea beverages by micellar electrokinetic chromatography.
abstract: Antioxidant properties and stimulating effects of green tea are related to its content of cathechins and xanthines; tea quality evaluation is based on organoleptic tests and on the presence of those components. In this work, by a MEKC method, eight cathechins and three xanthines were quantified in some tea-based beverages. The best separation was realized using a phosphate-borate running buffer, with sodium dodecyl sulfate as micellar agent. A 40 cm capillary, a temperature of 29 degrees C, a voltage of 30 kV, and UV detection at 200 nm were used. The method showed a very good sensitivity (limit of detection ranging from 0.0011 to 0.0051 microg/mL) and was applied to real tea samples to characterize their antioxidant content. Statistical studies were performed and showed a satisfactory reliability of the data.
authors.affiliation: Dipartimento di Scienze degli Alimenti, Università di Bologna, Via Ravennate 1020, Cesena (FC), CAP 47023, Italy. mbonoli@foodsci.unibo.it
authors.forename: Matteo
authors.initials: M
authors.lastname: Bonoli
authors.forename: Piero
authors.initials: P
authors.lastname: Colabufalo
authors.forename: Marco
authors.initials: M
authors.lastname: Pelillo
authors.forename: Tullia
authors.initials: T
authors.lastname: Gallina Toschi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Lercker
mesh_terms: D000975:Antioxidants / Q000032:analysis; D001628:Beverages / Q000032:analysis*; D002392:Catechin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013662:Tea / Q000737:chemistry*; D014970:Xanthines / Q000032:analysis*
chemical_list: D000975:Antioxidants; D013662:Tea; D014970:Xanthines; D002392:Catechin