title: Detection of immunoglobulin G to Crimean-Congo hemorrhagic fever virus in sheep sera by recombinant nucleoprotein-based enzyme-linked immunosorbent and immunofluorescence assays.
abstract: Crimean-Congo hemorrhagic fever virus is a tick-borne virus that causes severe hemorrhagic symptoms with an up to 50% mortality rate in humans. Wild and domestic animals, such as sheep, cattle and goats, are the reservoirs. The recombinant nucleoprotein-based Crimean-Congo hemorrhagic fever virus antibody detection systems for sheep sera were developed by enzyme-linked immunosorbent assay (ELISA) and an indirect immunofluorescence assay techniques. The samples used for evaluation were 80 sera collected from sheep in a Crimean-Congo hemorrhagic fever-endemic area (western part of the Xinjiang Uygur Autonomous Region) and 39 sera collected from sheep in a disease-free region (Shandong province, eastern China). The ELISA and indirect immunofluorescence assay using recombinant nucleoprotein of the virus proved to have high sensitivity and specificity for detecting the immunoglobulin G antibodies to the virus in sheep sera. Within this limited number of samples, the recombinant nucleoprotein-based ELISA and indirect immunofluorescence assay are considered to be useful tools for seroepidemiological study of virus infections in sheep sera.
authors.affiliation: Second Division of Viral Hemorrhagic Fever, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Changping, Beijing 102206, PR China.
authors.forename: Tang
authors.initials: T
authors.lastname: Qing
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saijo
authors.forename: Han
authors.initials: H
authors.lastname: Lei
authors.forename: Masahiro
authors.initials: M
authors.lastname: Niikura
authors.forename: Akihiko
authors.initials: A
authors.lastname: Maeda
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Ikegami
authors.forename: Wang
authors.initials: W
authors.lastname: Xinjung
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morikawa
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002681:China / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods* / Q000706:statistics & numerical data; D006367:HeLa Cells; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000276:immunology*; D006479:Hemorrhagic Fever, Crimean / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D009698:Nucleoproteins / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology; D014773:Virology / Q000379:methods / Q000706:statistics & numerical data
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D009698:Nucleoproteins; D011994:Recombinant Proteins; D014764:Viral Proteins