Identification of human dehydrodolichyl diphosphate synthase gene.
We isolated a cDNA encoding human dehydrodolichyl diphosphate (Dedol-PP) synthase and expressed the gene in a yeast mutant strain SNH23-7D, which is deficient in Dedol-PP synthase activity. The identity of the cloned enzyme was confirmed by functional complementation of SNH23-7D strain together with in vitro Dedol-PP synthase activity assay. Northern blot analysis indicated that testis and kidney expressed Dedol-PP synthase mRNA at high levels.
Endo
Shota
S
Institute of Multidisciplinary Research for Advanced Materials (IMRAM), Tohoku University, Katahira 2-1-1, Aoba-ku, 980-8577, Sendai, Japan.
Zhang
Yuan-Wei
YW
Takahashi
Seiji
S
Koyama
Tanetoshi
T
D019883:Alkyl and Aryl Transferases / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D004286:Dolichols / Q000096:biosynthesis; D015870:Gene Expression; D005816:Genetic Complementation Test; D006801:Humans; D007668:Kidney / Q000201:enzymology; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000201:enzymology; D015003:Yeasts / Q000378:metabolism
D018076:DNA, Complementary; D004286:Dolichols; D012333:RNA, Messenger; D019883:Alkyl and Aryl Transferases; C051389:dehydrodolichyl diphosphate synthetase