A novel EID-1 family member, EID-2, associates with histone deacetylases and inhibits muscle differentiation.
An EID-1 (E1A-like inhibitor of differentiation-1) inhibits differentiation by blocking the histone acetyltransferase activity of p300. Here we report a novel inhibitor of differentiation exhibiting homology to EID-1, termed EID-2 (EID-1-like inhibitor of differentiation-2). EID-2 inhibited MyoD-dependent transcription and muscle differentiation. Unlike EID-1, EID-2 did not block p300 activity. Interestingly, EID-2 associated with class I histone deacetylases (HDACs). The N-terminal portion of EID-2 was required for the binding to HDACs. This region was also involved in the transcriptional repression and nuclear localization, suggesting the importance of the involvement of HDACs in the EID-2 function. These results indicate a new family of differentiation inhibitors, although there are several differences in the biochemical mechanisms between EID-2 and EID-1.
Miyake
Satoshi
S
Department of Molecular Oncology, Tokyo Medical and Dental University, Graduate School of Medicine and Dentistry, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, Japan. miyake.monc@tmd.ac.jp
Yanagisawa
Yuka
Y
Yuasa
Yasuhito
Y
D017500:Adenovirus E1A Proteins / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000502:physiology*; D018797:Cell Cycle Proteins; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000502:physiology; D006655:Histone Deacetylases / Q000502:physiology*; D006801:Humans; D051797:Inhibitor of Differentiation Protein 2; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D032342:Muscle Cells / Q000166:cytology / Q000502:physiology*; D009687:Nuclear Proteins; D012097:Repressor Proteins
D017500:Adenovirus E1A Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C416925:EID1 protein, human; C475090:EID2 protein, human; D051797:Inhibitor of Differentiation Protein 2; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins; D006655:Histone Deacetylases