Genomic cloning and promoter analysis of a mouse anion exchanger 3 (AE3) gene.
The brain and cardiac isoforms of anion exchanger 3 (AE3) are considered to use their own promoters for their expression. However, little is known as to how the alternative transcription initiation is regulated. As a first step for elucidating the regulation, we obtained a genomic gene of mouse AE3. The 19-kbp clone contains about 6 kbp of 5' flanking region, 23 exons, and 22 introns. We have sequenced the whole region including introns and determined the intron-exon boundaries. Six amino acids are different from those deduced from the reported mouse AE3 cDNA. We measured a promoter activity of the 5' flanking region of the exon 1 for a brain type isoform and that of the exon C1 for a cardiac type isoform. The upstream region of the exon C1 indeed showed a promoter activity in rat cardiomyoblastic H9C2 cells, rat pheochromocyotoma PC12 cells, and human HeLa cells whereas the 5' flanking region of the exon 1 does not in HeLa cells, suggesting that the promoter for the cardiac type is rather ubiquitously active.
Iwaasa
Mitsutoshi
M
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Department of Nuerosurgery, Fukuoka University Faculty of Medicine, Fukuoka, Japan.
Tatewaki
Hideki
H
Ohno
Tetsuji
T
Okubo
Kenshi
K
Hamasaki
Naotaka
N
Kang
Dongchon
D
D000595:Amino Acid Sequence; D000818:Animals; D017920:Antiporters / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms / Q000235:genetics; D051381:Rats; D017422:Sequence Analysis, DNA
D017920:Antiporters; D020033:Protein Isoforms; C489237:SLC4A3 protein, human; C062002:Slc4a3 protein, mouse; C489238:Slc4a3 protein, rat