Detection of 1583 bp gene transcript in lymphocytes of muscular dystrophy patients.
DMD and BMD are X-lined recessive disorders. RAP-PCR was utilized to investigate differentially expressed gene transcripts in lymphocytes from DMD, BMD and normal individuals as possible diagnostic parameter. A 1583 bp transcript was found to be expressed in both DMD and BMD patients which was unrelated to the known dystrophin gene. This may prove helpful in determining the carrier status of DMD/BMD.
Prabhakar
S
S
Department of Neurology, Postgraduate Institute of Medical Education and Research, Chandigarh - 160 012, India.
Anand
A
A
D001483:Base Sequence / Q000235:genetics; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D020388:Muscular Dystrophy, Duchenne / Q000097:blood* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000097:blood* / Q000235:genetics*; D012016:Reference Values
D012313:RNA