title: Robo and Frazzled/DCC mediate dendritic guidance at the CNS midline.
abstract: Neuronal connectivity is established by the axo-dendritic polarity, correct guidance and targeting of neurons. Unlike for axons, the mechanisms responsible for directed outgrowth of dendrites are not well understood. Using single-cell labeling, we describe specific guidance defects in dendrites of identified neurons in frazzled, robo, netrin and commissureless mutant embryos of Drosophila melanogaster. We found that the cell-surface molecules Frazzled and Robo work as guidance molecules not only for axons but also for dendrites as they navigate within the CNS. Furthermore, we report that each neuron showed a cell-autonomous and independent use of guidance molecules.
authors.affiliation: Department of Cell and Structural Biology, University of Illinois, 601 South Goodwin Avenue, Urbana, Illinois 61801, USA.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Furrer
authors.forename: Susan
authors.initials: S
authors.lastname: Kim
authors.forename: Brian
authors.initials: B
authors.lastname: Wolf
authors.forename: Akira
authors.initials: A
authors.lastname: Chiba
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism; D002490:Central Nervous System / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D004625:Embryo, Nonmammalian / Q000294:innervation; D005456:Fluorescent Dyes; D008565:Membrane Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism; D009154:Mutation; D009414:Nerve Growth Factors / Q000172:deficiency / Q000235:genetics; D009419:Nerve Tissue Proteins; D000075386:Netrin Receptors; D000075388:Netrin-1; D000075385:Netrins; D011956:Receptors, Cell Surface / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins; D000097905:Roundabout Proteins
chemical_list: D029721:Drosophila Proteins; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D000075386:Netrin Receptors; D000075388:Netrin-1; D000075385:Netrins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D025521:Tumor Suppressor Proteins; D000097905:Roundabout Proteins; C110665:comm protein, Drosophila; C000617310:fra protein, Drosophila; C088894:netrin-2