Auxin regulated poly(A)polymerase activity in Cicer arietinum epicotyls.
There was a linear increase in poly (A+) polymerase activity in the C. arietinum epicotyls during germination. Six-day-old auxin treated seedlings showed about 3-4 fold stimulation in enzyme activity, accompanied with 3- fold rise in the relative abundance of poly (A+) RNA levels. Actinomycin D, cycloheximide, cordycepin and amino acid analogues caused dramatic decline in poly (A+) polymerase as well as poly (A+) RNA levels. It seems that auxin induced a de novo synthesis of this enzyme.
Praveen
S
S
Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110012.
Mehrotra
P K
PK
Prakash
S
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Kapoor
H C
HC
D000596:Amino Acids / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology; D029863:Cicer / Q000187:drug effects* / Q000201:enzymology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003839:Deoxyadenosines / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D018506:Gene Expression Regulation, Plant; D018525:Germination; D007210:Indoleacetic Acids / Q000494:pharmacology*; D011061:Poly A / Q000378:metabolism; D011062:Polynucleotide Adenylyltransferase / Q000302:isolation & purification / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012313:RNA / Q000378:metabolism; D012639:Seeds / Q000187:drug effects* / Q000201:enzymology
D000596:Amino Acids; D000970:Antineoplastic Agents; D003839:Deoxyadenosines; D007210:Indoleacetic Acids; D011500:Protein Synthesis Inhibitors; D003609:Dactinomycin; D011061:Poly A; D012313:RNA; D003513:Cycloheximide; D011062:Polynucleotide Adenylyltransferase; C058120:cordycepin