Ex vivo synergy of arachidonate-enriched serum with ceftazidime and amikacin on multidrug-resistant Pseudomonas aeruginosa.
Three multidrug-resistant strains of Pseudomonas aeruginosa were incubated ex vivo with sera sampled after a 10 min intravenous infusion of 25 mg/kg of arachidonic acid (AA) in 10 rabbits in the presence of ceftazidime and amikacin. Lipid peroxidation was assessed during bacterial growth. A statistically significant decrease in bacterial cells was found by the interaction of antimicrobials and serum sampled in the middle of infusion and 15 and 30 min after infusion of AA and was accompanied by elevated levels of malonodialdehyde. This effect of AA is probably attributed to lipid peroxidation and raises the possibility of its application in experimental infections.
Giamarellos-Bourboulis
Evangelos J
EJ
4th Department of Internal Medicine, Sismanoglio General Hospital, 1 Sismanogliou Str., 151 26 Maroussi Attikis, Grece. giamarel@internet.gr
Plachouras
Diamantis
D
Skiathitis
Sotirios
S
Raftogiannis
Maria
M
Dionyssiou-Asteriou
Amalia
A
Dontas
Ismini
I
Karayannacos
Panayotis E
PE
Giamarellou
Helen
H
D000583:Amikacin / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000097:blood / Q000494:pharmacology*; D002442:Ceftazidime / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003470:Culture Media; D024901:Drug Resistance, Multiple, Bacterial; D004357:Drug Synergism; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D011817:Rabbits; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D003470:Culture Media; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D016718:Arachidonic Acid; D008315:Malondialdehyde; D000583:Amikacin; D002442:Ceftazidime