The novel differentiation of human blood mononuclear cells into CD1a-negative dendritic cells is stimulated in the absence of exogenous cytokines by an extract prepared from pinecones.
The production of dendritic cells, both in-vivo and in-vitro, has become the intense focus of research activities. Common to many of these production protocols is the use of cytokines, typically granulocyte-monocyte colony stimulating factor and either interleukin 4 or tumor necrosis factor alpha or a combination of all three. Herein, we report our findings that a proprietary pinecone extract is capable of in a dose-dependent manner, and in the absence of exogenous cytokines, the rapid differentiation from peripheral blood mononuclear cells of mature CD1a-negative dendritic cells.
Bradley
W G
WG
Eckerd College, 4200 54th Avenue South, St Petersburg, FL 33711, USA. gbradley@tampabayresearch.org
Widen
R H
RH
Weiser
A M
AM
Powers
J J
JJ
Fountain
L B
LB
Punjwani
P
P
Lofgren
S M
SM
Hadzic
T
T
Klein
R
R
Green
W H
WH
Tanaka
A
A
D000818:Animals; D018949:Antigens, CD1 / Q000032:analysis*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology*; D004731:Endotoxins / Q000494:pharmacology; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D018950:Lipopolysaccharide Receptors / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D028223:Pinus / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*
D018949:Antigens, CD1; C419393:CD1a antigen; C408982:CPG-oligonucleotide; D004731:Endotoxins; D018950:Lipopolysaccharide Receptors; D009838:Oligodeoxyribonucleotides; D010936:Plant Extracts