Distribution of dopamine D2 receptor mRNAs in the brain and the pituitary of female rainbow trout: an in situ hybridization study.
The distribution of D(2)R (dopamine D(2) receptor) mRNAs was studied in the forebrain of maturing female rainbow trout by means of in situ hybridization using a (35)S-labeled riboprobe (810 bp) spanning the third intracytoplasmic loop. A hybridization signal was consistently obtained in the olfactory epithelium, the internal cell layer of the olfactory bulbs, the ventral and dorsal subdivisions of the ventral telencephalon, and most preoptic subdivisions, with the notable exception of the magnocellular preoptic nucleus, and the periventricular regions of the mediobasal hypothalamus, including the posterior tuberculum. In the pituitary, the signal was higher in the pars intermedia than in the proximal and the rostral pars distalis, but no obvious correspondence with a given cell type could be assigned. Labeled cells were also located in the thalamic region, some pretectal nuclei, the optic tectum, and the torus semicircularis. These results provide a morphologic basis for a better understanding on the functions and evolution of the dopaminergic systems in lower vertebrates.
Vacher
Coralie
C
Endocrinologie Moléculaire de la Reproduction, UMR CNRS 6026, Campus de Beaulieu, 35042 Rennes cedex, France.
Pellegrini
Elisabeth
E
Anglade
Isabelle
I
Ferriére
François
F
Saligaut
Christian
C
Kah
Olivier
O
D000818:Animals; D005260:Female; D017403:In Situ Hybridization; D017686:Oncorhynchus mykiss; D010902:Pituitary Gland / Q000737:chemistry*; D016548:Prosencephalon / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis; D017448:Receptors, Dopamine D2 / Q000032:analysis* / Q000235:genetics
D012333:RNA, Messenger; D017448:Receptors, Dopamine D2