[The study on clinical significance of human herpesvius 6 infection of NPC tissues].
OBJECTIVE
To study the infection of human herpesvirus 6(HHV-6) in NPC and its role in the carcinogensis of NPC.


METHODS
By using polymerase chain reaction (PCR) and in situ hybridization (ISH) HHV-6 and EBV DNA to from the paraffin-embedded tissues of normal nasopharynx precancerous nasopharynx and nasopharyngeal carcinoma. EBV LMP1 was also detected in 36 tissues of NPC by immunohistochemistry (IHC).


RESULTS
HHV-6 DNA PCR were detected in 30.9% (13/42) of tissues of NPC, 4.7% (1/21) of precancerous nasopharynx and non in the normal nasopharynx. Within the same tissues, EBV DNA positive rates were 95.2% (40/42), 66.6% (14/21) and 25.9% (7/27), respectively. By using ISH, HHV-6 DNA were detected only in 11 NPC tissues out of 13 HHV-6 DNA positive NPC cases and EBV DNA were detected in 47.6% (10/21) of precancerous nasopharynx tissues and 85.7% (36/42) of NPC cases. EBV LMP1 expression were detected in 47.2% (17/36) of NPC tissues.


CONCLUSIONS
NPC tissues can be infected with both HHV-6 and EBV, HHV-6 infection is correlated with the expression of EBV LMP1, suggesting that HHV-6 plays a direct/or indirect role in carcinogenesis of NPC via upregulating the expression of EBV LMP1.
Chen
X
X
Shenzhen Eastlake Hospital, Shenzhen, 518020.
Jian
S
S
Wang
H
H
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D003598:Cytoskeletal Proteins; D004279:DNA, Viral / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D015654:Herpesvirus 6, Human / Q000302:isolation & purification*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; D009303:Nasopharyngeal Neoplasms / Q000821:virology*; D011230:Precancerous Conditions / Q000821:virology*; D019349:Roseolovirus Infections
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D004279:DNA, Viral; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; C484380:PDLIM7 protein, human