[Effect of Panax notoginseng saponins on human kidney fibroblast].
OBJECTIVE
To study the effect of Panax notoginseng saponins (PNS) on cell proliferation, type I collagen secretion and integrin beta 1 expression in human kidney fibroblast (KFB).


METHODS
KFB were cultured and stimulated by PNS in vitro. The cell proliferation, type I collagen secretion and integrin beta 1 expression in KFB were measured by methyl thiazolyl tetrazolium (MTT), enzyme-linked immunoabsorbent assay (ELISA) and flowcytometry respectively.


RESULTS
Within its optimal concentration range and effective time range, PNS could obviously inhibit the proliferation, type I collagen secretion and integrin beta 1 expression (all P < 0.05) in human KFB.


CONCLUSIONS
PNS would possibly be an effective drug for renal interstitial fibrosis prevention and treatment.
Wei
Ying
Y
Red-Cross Hospital of Yunnan, Kunming 650021.
Fan
Jun-ming
JM
Pan
Li-ping
LP
D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects*; D005355:Fibrosis / Q000517:prevention & control; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism; D007668:Kidney / Q000166:cytology* / Q000473:pathology; D005894:Panax / Q000737:chemistry*; D012503:Saponins / Q000494:pharmacology*
D024042:Collagen Type I; D019012:Integrin beta1; D012503:Saponins