[Aedes albopictus: cloning and identification of the acetylcholinesterase gene fragment from the mosquito].
OBJECTIVE
To isolate, clone and identify the acetylcholinesterase (AChE) fragment from the mosquito, Aedes albopictus, in relation to exploring mechanism of insectide resistance.


METHODS
The genome DNA extracted from the mosquito was used for degenerate polymerase chain reaction (PCR) and the two pairs of oligonucleotides encoding the highly conserved protein sequences were used as primers. The reaction products were cloned to T-vector and transfected into E. coli JM 109. The replicative form DNA of recombinant vector extracted from E. coli JM 109 through alkalilysis was identified by the methods of digestion with EcoR I and Sal I and PCR.


RESULTS
The products of degenerate primers polymerase chain reaction were obtained and the identified clone belongs to the AChE fragment of the mosquito.


CONCLUSIONS
The clone was identified as the AChE fragment of Aedes albopictus.
Wu
M W
MW
Department of Parasitology, Medical College, Jinan University, Guangzhou 510632.
Zhang
L M
LM
Huang
J L
JL
Zhou
G L
GL
Wu
Y
Y
Zhao
S X
SX
D000110:Acetylcholinesterase / Q000235:genetics*; D000330:Aedes / Q000201:enzymology / Q000235:genetics*; D000818:Animals; D003001:Cloning, Molecular; D007305:Insecticide Resistance / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction
D000110:Acetylcholinesterase