[Study on human eye ciliary muscule cell culture and biologic characteristics].
OBJECTIVE
We cultured human ciliary muscle [HCM] cells to study their growth, ultrastructure, immunohistochemistry and functional characters.


METHODS
HCM cells from 10 young donor eyes were cultured with collagenase IV digestion procedures in vitro, the cells were identified by eletronmicroscope and immunohistochemistry assay, their function were studied by single-cell-contraction assay.


RESULTS
The cells were passed and grew in Hill-Valley pattern after conflunet; abundant filaments were presented under electronmicroscope. In Desmin protein immunohistochemistry study, the cultured cells were stained positive; in tissue sections, HCM cells stained positive, vascular smooth muscle stained positive weakly, but fibroblast cells and endothelial cells stained negative. 10(-3) M Carbachol could induce the cultured cells contract, this effect was antagonized by 10(-3) M Atropine.


CONCLUSIONS
We successfully cultured HCM cells, which were able to contract.
Huang
W
W
Zhongshan Opthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Peng
D
D
Zeng
S
S
Qiu
P
P
Li
S
S
Zheng
S
S
D002478:Cells, Cultured; D002924:Ciliary Body / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D017364:Collagenases / Q000378:metabolism; D006801:Humans; D009130:Muscle, Smooth / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure
D017364:Collagenases