[Application of RAPD in the taxonomy of the genus Fritillaria].
OBJECTIVE
To provide the molecular data for the right application of the Chinese medicine "Beimu".


METHODS
Using the technology of RAPD, we studied the relationship among 12 samples of Beimu.


RESULTS
The total genomic DNA of all the samples are about 21 Kb in size. Among 20 primers used, the five primers can reatedly generate a certain specified amplified band type, 27 bands were recored from all amplified products and 25 polymorphic fragments were found in it. The size of amplified fragments is between 450 bp and 1904 bp.


CONCLUSIONS
The similarity within species is higher than those between species. The relationship of Fritillaria anhuiensis and F. puqiensis is the farest, while F. thunbergii and F. puqiensis is the closest.
Bian
Y
Y
China Pharmaceutical University, Nanjing 210038.
Li
P
P
Gao
Z
Z
Wang
Y
Y
Zhou
K
K
Tsim
K W
KW
Dong
T
T
D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics*; D031410:Fritillaria / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D010946:Plants, Medicinal / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity
D017931:DNA Primers; D018744:DNA, Plant