Taxus metabolomics: methyl jasmonate preferentially induces production of taxoids oxygenated at C-13 in Taxus x media cell cultures.
Cells from suspension cultures of Taxus cuspidata were extracted with pentane as a source of relatively non-polar taxoids. Of the 13 taxoids identified in this fraction, eight were oxygenated at C-14 and two had not been previously described. These taxoids, along with existing taxoid standards, were employed to profile the metabolites of Taxus x media cv. Hicksii cell suspension cultures induced with methyl jasmonate to produce paclitaxel (Taxol). The majority of the taxoid metabolites produced in these induced cultures were oxygenated at C-13, and not C-14.
Ketchum
Raymond E B
RE
Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA. rketchum@wsu.edu
Rithner
Christopher D
CD
Qiu
Deyou
D
Kim
You Sun
YS
Williams
Robert M
RM
Croteau
Rodney B
RB
D000085:Acetates / Q000494:pharmacology*; D000470:Alkaloids / Q000378:metabolism*; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D054883:Oxylipins; D020946:Taxus / Q000737:chemistry / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*
D000085:Acetates; D000470:Alkaloids; D003517:Cyclopentanes; D054883:Oxylipins; C072239:methyl jasmonate