Enhancement of monoclonal antibody production by lysine-containing peptides.
In the search for peptides that could effectively enhance the monoclonal antibody production of a model hybridoma, the performance of five lysine-containing peptides was compared. The capacity of the peptides to enhance the monoclonal antibody yield correlated with their growth-suppressing activity. No correlation of the production-enhancing activity with the character of the distribution of cell-cycle phases could be found. All of the tested peptides, including the negative control peptide Gly-Phe-Gly, altered the cell-cycle phases distribution in favor of the proportion of the S phase. The peptides added to the hybridoma culture were found to be gradually decomposed into dipeptides and free amino acids. Among the set of tested lysine-containing di- to pentapeptides, the best results were obtained with the tripeptide Gly-Lys-Gly. The growth-suppressing and production-enhancing capacity of this peptide supplement was obviously associated with the temporary presence of the intact peptide molecule in the culture media, because the addition of a mixture of free amino acids constituting this peptide, i.e., glycine and lysine, displayed a different effect-a slight promotion of cell growth.
Franek
Frantisek
F
Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Radiova 1, CZ-10227 Prague 10, Czech Republic. franek@biomed.cas.cz
Eckschlager
Tomás
T
Katinger
Hermann
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000917:Antibody Formation / Q000187:drug effects; D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D006825:Hybridomas / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D008239:Lysine / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D009842:Oligopeptides / Q000737:chemistry / Q000145:classification* / Q000494:pharmacology*; D010455:Peptides / Q000737:chemistry / Q000145:classification / Q000494:pharmacology; D011107:Polylysine / Q000494:pharmacology; D011786:Quality Control
D000911:Antibodies, Monoclonal; D009842:Oligopeptides; D010455:Peptides; D011107:Polylysine; D008239:Lysine