title: [Study on chemical constituents of rhizome of Anemone raddeana].
abstract: OBJECTIVE
  To investigate the chemical constituents of the rhizome of Anemone raddeana Regel, so as to find new active compounds.
  
  
  METHODS
  The ethanol extracts of the rhizome of Anemone raddeana were obtained by silica column, HPLC. The structures of the compounds were elucidated by means of physico-chemical method and spectral analysis (IR, FAB-MS, 1HNMR, 13CNMR, DEPT, 1H-1H COSY, HMQC, HMBC).
  
  
  RESULTS
  Nine compounds were isolated and identified as 27-hydroxyolean-12(13)-en-28-oic acid-3-0-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranoside (1), eleutheroside K (2), Oleanolic acid-3-O-alpha-L-rhamnopyranosyl-(1-->2)-[beta-D- glucopyranosyl-(1-->4)]-alpha-L-arabinopyranoside (3), betulin (4), betulic acid (5), acetyloleamolic acid (6), evonymitol (7), oleamolic acid (8) and diosgenin (9).
  
  
  CONCLUSIONS
  Compound 1 is a new compound, named raddeanoside 12. Compounds 3-7 were isolated from this plant for the first time.
authors.affiliation: Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110016, China. jincailu99@yahoo.com
authors.forename: Jin-cai
authors.initials: JC
authors.lastname: Lu
authors.forename: Bei-bei
authors.initials: BB
authors.lastname: Xu
authors.forename: Xin-yan
authors.initials: XY
authors.lastname: Zhang
authors.forename: Qi-shi
authors.initials: QS
authors.lastname: Sun
mesh_terms: D031883:Anemone / Q000737:chemistry*; D028001:Eleutherococcus; D015394:Molecular Structure; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification; D000094062:Betulinic Acid
chemical_list: C485708:27-hydroxyolean-12(13)-en-28-oic acid-3-O-rhamnopyranosyl-1-2-arabinopyranoside; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C029073:eleutherosides; C002503:betulin; D000094062:Betulinic Acid