[Morphological study on three-dimensional culture of rat muscle satellite cells under cyclic mechanical stretch].
OBJECTIVE
To study the effect of mechanical stretch on the morphologic change of rat muscle satellite cell in the three-dimensional culture.


METHODS
Based on the apparatus for three-dimensional cell cultures under a cyclic mechanical strain, a specific stretch pattern (10% elongation, 10 stretches/min for 10 min of each hour  48 h total) was applied to cell-scaffolding composites.


RESULTS
Under the stretch pattern, the shape of satellite cells changed to oblate and spread to the direction of the stretch. Furthermore, the myotube was observed. On the contrary, the satellite cells spread to the all direction, and the formation of the myotube was not been found in the control group.


CONCLUSIONS
Cyclic mechanical stretch is helpful for the formation of the ideal directivity and these results are compatible with a significant role for the stretch in tissue-engineered muscle construction.
Xiong
Shao-hu
SH
Key Laboratory of Tissue Construction and Detection of Guangdong Province, First Military Medical University, Guangzhou 510515, China. ln-xsh@sohu.com
Li
Ning
N
Zhao
Jin-mei
JM
Yu
Lei
L
Liao
Hua
H
Qin
Ting-wu
TW
Zhong
Shi-zhen
SZ
D000818:Animals; D001696:Biomechanical Phenomena; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D009132:Muscles / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D023822:Tissue Engineering