Novel mass spectrometric immunoassays for the rapid structural characterization of plasma apolipoproteins.
Novel mass spectrometric immunoassays (MSIAs) for the isolation and structural characterization of plasma apolipoprotein A-I (apoA-I), apoA-II, and apoE have been developed. The assays combine selective isolation of apolipoprotein species via affinity capture with mass-specific detection using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. In application, plasma (from 50 microl of whole blood drawn from individuals, using finger lancet) was addressed with affinity pipette tips derivatized with antibodies toward the specific apolipoprotein. The time required for each assay was approximately 15 min, less if assays on multiple individuals were performed in parallel. In a brief study of five individuals, several recently reported apoA-II variants were identified and observed consistently in all individuals. Additionally, the apoE phenotype of E3/E3 was observed in three of the individuals, and E2/E3 and E3/E4 observed in the remaining two individuals, the latter of whom suffers from Alzheimer's disease. Overall, the MSIA approach offers a rapid, sensitive, and highly accurate means of profiling apolipoproteins from small volumes of plasma.
Niederkofler
Eric E
EE
Intrinsic Bioprobes, Inc., 625 South Smith Road, Suite 22, Tempe, AZ 85281, USA.
Tubbs
Kemmons A
KA
Kiernan
Urban A
UA
Nedelkov
Dobrin
D
Nelson
Randall W
RW
D000328:Adult; D000368:Aged; D016632:Apolipoprotein A-I / Q000097:blood / Q000737:chemistry; D016633:Apolipoprotein A-II / Q000097:blood / Q000737:chemistry; D001053:Apolipoproteins / Q000097:blood* / Q000737:chemistry*; D001057:Apolipoproteins E / Q000097:blood / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*
D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D001053:Apolipoproteins; D001057:Apolipoproteins E