title: In vivo mapping of fractional plasma volume (fpv) and endothelial transfer coefficient (Kps) in solid tumors using a macromolecular contrast agent: correlation with histology and ultrastructure.
abstract: Contrast-enhanced MRI, immunostaining and electron microscopy were used to detect areas of intense angiogenesis in experimental tumors. This work was also aimed at evaluating the possible effect of the surrounding tissues on tumor microvasculature and at studying the penetration of macromolecules in avascular areas. Human colon carcinoma cells were implanted in subcutaneous tissue of nude mice. Dynamic T(1)-weigthed 3D pulse sequences were acquired before and after administration of Gd-DTPA-albumin to obtain parametric maps of fractional plasma volume (fpv) and transendothelial permeability (Kps). The maps suggested that tumor can be subdivided into 4 zones located in the peripheral rim (zones I-II) or in the core (zones III-IV) of the tumor itself. Significant differences (p<0.001) were found in the values of Kps and fpv of zones I-II with respect to zones III-IV. In the peripheral rim, permeability was significantly higher (p<0.01) in the muscle-peripheral region (zone I) with respect to the skin-peripheral region (zone II). In areas with high Kps, histological and ultrastructural examination revealed clusters of newly formed vessels and signs of intense permeability. Numerous vascular vesicular organs were visible in these areas. In the tumoral core, analysis of the microcirculatory parameters revealed regions with mild permeability (zone III) and regions with negligible permeability (zone IV). These 2 zones were discriminated by the average value of Kps (p<0.05), while their fpv was not significantly different. Upon histological examination, the tumoral core exhibited necrotic areas; CD31 immunocytochemistry exhibited that it was diffusely hypovascularized with large avascular areas. Upon ultrastructural examination, capillaries were rarely visible and exhibited signs of endothelial cell damage. The results suggest that segmentation based on microvascular parameters detects in vivo zones characterized by immunocytochemical and ultrastructural aspects of intense angiogenesis. The finding that a certain amount of contrast agent penetrates in the tumoral core suggests that high oncotic and hydrostatic pressure only partially hinders the penetration of macromolecules.
authors.affiliation: Dipartimento di Scienze Morfologico-Biomediche, Sezione di Anatomia ed Istologia, Universitá di Verona, Verona, Italy.
authors.forename: Pasquina
authors.initials: P
authors.lastname: Marzola
authors.forename: Paolo
authors.initials: P
authors.lastname: Farace
authors.forename: Laura
authors.initials: L
authors.lastname: Calderan
authors.forename: Caterina
authors.initials: C
authors.lastname: Crescimanno
authors.forename: Ernesto
authors.initials: E
authors.lastname: Lunati
authors.forename: Elena
authors.initials: E
authors.lastname: Nicolato
authors.forename: Donatella
authors.initials: D
authors.lastname: Benati
authors.forename: Anna
authors.initials: A
authors.lastname: Degrassi
authors.forename: Andrea
authors.initials: A
authors.lastname: Terron
authors.forename: Jan
authors.initials: J
authors.lastname: Klapwijk
authors.forename: Enrico
authors.initials: E
authors.lastname: Pesenti
authors.forename: Andrea
authors.initials: A
authors.lastname: Sbarbati
authors.forename: Francesco
authors.initials: F
authors.lastname: Osculati
mesh_terms: D000818:Animals; D003287:Contrast Media; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D007089:Image Enhancement; D046911:Macromolecular Substances; D008279:Magnetic Resonance Imaging; D051379:Mice; D009374:Neoplasms, Experimental / Q000098:blood supply* / Q000648:ultrastructure; D009389:Neovascularization, Pathologic / Q000175:diagnosis*; D010539:Permeability; D010953:Plasma Volume
chemical_list: D003287:Contrast Media; D046911:Macromolecular Substances