Contractile activity of human decidual stromal cells.
We previously demonstrated that human decidual stromal cells (DSC), the main cellular component of the decidua, are similar in antigen phenotype and structure to myofibroblasts, cells with contractile activity. In this work we isolated and maintained DSC in fibroblast medium, in which these cells show a stable phenotype similar to that of DSC in vivo. Flow cytometric observations showed that most DSC expressed alpha-smooth muscle (alpha-SM) actin, an intermediate filament that is considered a marker of myofibroblasts and is responsible for the contractile activity of these cells. alpha-SM actin mRNA was detected by RT-PCR in these cells. The contractile activity of DSC was determined by the gel contraction assay; we found that TGF beta 1 and platelet-derived-growth factor, cytokines that are known to be inducers of myofibroblast contractility, also induced contractility of DSC. IL-2, a Th1 cytokine-related with spontaneous abortion, also activated DSC contractility. Our results confirmed that DSC are phenotypically and functionally related with myofibroblast.
Kimatrai
María
M
Unidad de Inmunología, Facultad de Medicina, Universidad de Granada, 18012 Granada, Spain.
Oliver
Carmen
C
Abadía-Molina
Ana Clara
AC
García-Pacheco
José Marcos
JM
Olivares
Enrique G
EG
D000022:Abortion, Spontaneous / Q000503:physiopathology; D000199:Actins / Q000235:genetics; D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology; D002478:Cells, Cultured; D003656:Decidua / Q000166:cytology* / Q000502:physiology*; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology*; D014590:Uterine Contraction / Q000187:drug effects / Q000502:physiology*
D000199:Actins; D000970:Antineoplastic Agents; D007376:Interleukin-2; D012333:RNA, Messenger