Stress-like responses to common procedures in individually and group-housed female rats.
The objective of this study was to assess the cardiovascular function and behavior of female Sprague-Dawley rats housed individually or with one or three cage mates under resting conditions and when subjected to common husbandry and experimental procedures and potentially stressful olfactory stimuli. Heart rate (HR) and mean arterial blood pressure (MAP) were assessed continuously by using radiotelemetry and are reported for the following periods: for 1 hour each day prior to any human interaction; for 12 h each day during the dark phase of the 12:12-h light: dark photoperiod; and for 2 h before and 3 h after acute husbandry and experimental procedures. Home-cage behaviors (sleeping, awake but not moving, moving, rearing, and grooming) were scored once each minute for 15 min before and 45 min after the acute procedures. Mean resting HR values in the mornings prior to human contact were significantly (P < 0.05) lower in rats housed four per cage than animals housed alone or with one cage mate, whereas MAP during this period was lowest in rats housed two per cage. Nocturnal HRs were highest in rats housed two per cage, whereas nocturnal MAP did not differ significantly between housing groups. When rats were subjected to acute husbandry and experimental procedures, HRs increased 80 to 180 beats per min (bpm) above a baseline of 300 to 325 bpm and were significantly (P < 0.05) increased for periods of 30 to 90 min after the procedures. MAP showed increases that were proportionately the same as those in HR. Group housing often, but not always, reduced these cardiovascular responses. Procedure-induced arousal behaviors occurred in all housing groups after the acute husbandry and experimental procedures, but the occurrence of these behaviors was less frequent and of shorter duration in group-housed rats than rats housed alone. In light of these results, we conclude that under resting conditions group housed rats were somewhat less stressed than were rats housed alone. Further, we conclude that common procedures induce significant stress-like responses in female rats, and the magnitude and duration of these responses are reduced by group housing.
Sharp
Jody
J
Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
Zammit
Timothy
T
Azar
Toni
T
Lawson
David
D
D000822:Animal Husbandry; D000818:Animals; D000830:Animals, Laboratory; D001522:Behavior, Animal; D001794:Blood Pressure; D005260:Female; D006234:Handling, Psychological; D006339:Heart Rate; D006799:Housing, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D012376:Rodent Diseases / Q000503:physiopathology*; D012934:Social Isolation; D013312:Stress, Physiological / Q000503:physiopathology / Q000662:veterinary*