Enhanced serum neutrophil chemotactic activity was noted in both early and late asthmatic responses during lysine-aspirin bronchoprovocation test in ASA-sensitive asthmatic patients.
To investigate the pathogenic mechanism of late asthmatic response in comparison to early asthmatic response, changes of serum neutrophil chemotactic activity (NCA) using the Boyden chamber method and histamine level using the automated fluorometric analyzer were observed in 13 aspirin (ASA)-sensitive asthma subjects (group I: 7 early responders and group II: 6 dual responders) during lysine aspirin bronchoprovocation test (L-ASA BPT). Sera were collected before, and 30 min and 240 min after L-ASA BPT. Serum NCA increased significantly after 30 min (p=0.02) and decreased significantly at 240 min (p=0.02) in group I, while serum NCA of group II increased significantly at 30 min (p=0.04), tending to increase further up to 240 min with no statistical significance. NCA at 240 min in group II subjects was significantly higher than baseline NCA (p=0.02). The serum NCAs collected before and 240 min were significantly higher in group II than in group I (p<0.05, respectively). There were no significant changes in serum histamine levels during L-ASA BPT in both groups. NCA derived from mast cell may contribute to the development of early asthmatic response induced by L-ASA inhalation. There may be a possible involvement of NCA derived from mononuclear cells during late asthmatic response.
Kim
Sun-Sin
SS
Department of Allergy & Clinical Immunology, Ajou University School of Medicine, San-5, Wonchondong, Paldalgu, Suwon 442-749, Korea.
Park
Hae-Sim
HS
Yoon
Ho-Joo
HJ
Lee
Young-Mok
YM
Lee
Soo-Keol
SK
Nahm
Dong-Ho
DH
D000328:Adult; D000368:Aged; D001241:Aspirin / Q000009:adverse effects; D001249:Asthma / Q000097:blood* / Q000139:chemically induced; D001985:Bronchial Provocation Tests; D002630:Chemotactic Factors / Q000097:blood* / Q000378:metabolism; D002633:Chemotaxis / Q000187:drug effects*; D005260:Female; D006632:Histamine / Q000097:blood; D006801:Humans; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000502:physiology; D008239:Lysine; D008297:Male; D008407:Mast Cells / Q000378:metabolism; D016210:Methacholine Chloride; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects*; D013997:Time Factors
D002630:Chemotactic Factors; D016209:Interleukin-8; D016210:Methacholine Chloride; D006632:Histamine; D008239:Lysine; D001241:Aspirin