title: High plasma osteopontin level and its relationship with interleukin-12-mediated type 1 T helper cell response in tuberculosis.
abstract: Osteopontin (OPN, also known as Eta-1), a noncollagenous matrix protein produced by macrophages and T lymphocytes, is expressed in granulomatous lesions caused by Mycobacterium tuberculosis infection. In the present study, we compared plasma concentrations of OPN in patients with active pulmonary tuberculosis with those of healthy control subjects and patients with sarcoidosis, another disease associated with granuloma formation. Plasma OPN levels were significantly higher in patients with tuberculosis (n = 48) than in control subjects (n = 34) and patients with sarcoidosis (n = 20). OPN levels correlated well with severity of pulmonary tuberculosis, as indicated by the size of lung lesions on chest X-ray films. Furthermore, chemotherapy resulted in a significant fall in plasma OPN levels. In patients with tuberculosis, plasma OPN concentrations correlated significantly with those of interleukin (IL)-12. In vitro experiments showed that OPN production by peripheral blood mononuclear cells infected with Mycobacterium bovis bacillus Calmette-Guérin preceded the synthesis of IL-12 and interferon-gamma and that the neutralizing anti-OPN monoclonal antibody significantly reduced the production of IL-12 and interferon-gamma. Our results suggest that OPN may be involved in the pathologic process associated with active pulmonary tuberculosis by inducing IL-12-mediated type 1 T helper cell responses.
authors.affiliation: First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Koguchi
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Kawakami
authors.forename: Kaori
authors.initials: K
authors.lastname: Uezu
authors.forename: Kiyoyasu
authors.initials: K
authors.lastname: Fukushima
authors.forename: Sigeyuki
authors.initials: S
authors.lastname: Kon
authors.forename: Masahiro
authors.initials: M
authors.lastname: Maeda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakamoto
authors.forename: Isoko
authors.initials: I
authors.lastname: Owan
authors.forename: Mutsuo
authors.initials: M
authors.lastname: Kuba
authors.forename: Norifumi
authors.initials: N
authors.lastname: Kudeken
authors.forename: Masato
authors.initials: M
authors.lastname: Azuma
authors.forename: Satomi
authors.initials: S
authors.lastname: Yara
authors.forename: Takashi
authors.initials: T
authors.lastname: Shinzato
authors.forename: Futoshi
authors.initials: F
authors.lastname: Higa
authors.forename: Masao
authors.initials: M
authors.lastname: Tateyama
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Kadota
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mukae
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kohno
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Uede
authors.forename: Atsushi
authors.initials: A
authors.lastname: Saito
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000995:Antitubercular Agents / Q000008:administration & dosage; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D018664:Interleukin-12 / Q000032:analysis / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D053495:Osteopontin; D011379:Prognosis; D011446:Prospective Studies; D012016:Reference Values; D017565:Sarcoidosis, Pulmonary / Q000097:blood* / Q000175:diagnosis / Q000188:drug therapy; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D012795:Sialoglycoproteins / Q000097:blood*; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*; D018417:Th1 Cells / Q000378:metabolism; D014397:Tuberculosis, Pulmonary / Q000097:blood* / Q000175:diagnosis / Q000188:drug therapy
chemical_list: D000995:Antitubercular Agents; D015415:Biomarkers; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D053495:Osteopontin; D018664:Interleukin-12