A fiber-FISH contig spanning the non-recombining region of the human Y chromosome.
Using fluorescence in-situ hybridization on interphase chromatin fibers (fiber-FISH), we have constructed an overlapping fiber-FISH contig spanning the non-recombining region of the human Y chromosome (NRY). We first established a standard FISH-signal pattern for a distinct panel of DNA clones on prometaphase Y chromosomes in six healthy fertile men. Clones in the panel were selected from all R-bands as well as deletion intervals 1 through 7 plus PAR1 and PAR2 of the human Y chromosome. We next used signals of these marker clones to build a fiber-FISH contig for the multicopy gene families, CDY, DAZ, RBMY, TSPY and XKRY, along the NRY. Our fiber-FISH contig of human NRY may help to close the four gaps that still exist in the current physical map of the human Y chromosome. Furthermore, it provides a more complete picture with respect to the positions and arrangements of the multicopy gene families along the human NRY.
Röttger
Susanne
S
Institute of Human Genetics and Anthropology, University of Freiburg, Breisacher Str. 33, 79106 Freiburg, Germany.
Yen
Pauline H
PH
Schempp
Werner
W
D002843:Chromatin / Q000235:genetics*; D002871:Chromosome Banding; D002874:Chromosome Mapping; D041322:Chromosomes, Human, Y / Q000235:genetics*; D015342:DNA Probes; D017353:Gene Deletion; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D008297:Male; D008677:Metaphase; D005810:Multigene Family / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics*
D002843:Chromatin; D015342:DNA Probes; D009687:Nuclear Proteins