Retrograde labeling of adult rat retinal ganglion cells with the flurogold.
OBJECTIVE
To study the densities and distribution of retinal ganglion cells(RGC) in adult rat retinae with flurogold(FG) labeling retogradely.


METHODS
FG was injected to the superior colliculi(SC) and dorsal lateral geniculate nuclei (dLGN) in adult rats and the retinae were examined by fluorescence microscopy at various periods of time.


RESULTS
FG-labelled RGC were observed in the retina as early as 3 days after application of FG. The labelled cells gradually increased in density, reached 95% of the maximal number on days 7 and the maximal number on days 30. The density of labelled cells was higher in the posterior pole than in the peripheral area. The fluorescence intensity in labelled cells maintained up to 60 days.


CONCLUSIONS
The FG retrograde labeling method is reliable and effective for quantity of RGC.
Huang
W
W
Department of Ophthalmology, Xijing Hospital, 4th Military Medical University, Xian 710032, China.
Hui
Y
Y
Zhang
M
M
D000818:Animals; D002452:Cell Count; D005456:Fluorescent Dyes; D005829:Geniculate Bodies; D008297:Male; D008856:Microscopy, Fluorescence; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012160:Retina / Q000166:cytology*; D012165:Retinal Ganglion Cells / Q000166:cytology*; D013194:Staining and Labeling; D013266:Stilbamidines; D013477:Superior Colliculi
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines