[Tolerance of HepG2 and HepG2.2.15 cell lines to different apoptotic stimuli].
OBJECTIVE
To elucidate the effect of HBV on hepatocyte apoptosis.


METHODS
The HepG2 cells and the HBV transfected HepG2.2.15 cells were cultured with MIX or ActD or cultured in DMEM medium without serum, The apoptosis was examined with FCM.


RESULTS
The apoptotic rates of HepG2.2.15 cells at 24 h and 48 h after MTX addition were 10.8% and 13.3%, at 24 h and 48 h after Act D addition were 16.8% and 37.7%, and at 4th and 6th day after serum withdrawal were 13.2% and 14.8%, respectively. While those of HepG2 cells were 12.6% and 65.3%, 44.5% and 89.7%, and 19.8% and 28.8%, correspondingly.


CONCLUSIONS
HepG2.2.15 cell was tolerant to these apoptotic stimuli, and it might be inferred that HBV inhibits hepatocyte apoptosis.
Zhu
Y
Y
Department of Infectious Diseases of Nanfang Hospital, First Military Medical University, Guangzhou 510515.
Luo
K
K
D017209:Apoptosis; D002478:Cells, Cultured; D006515:Hepatitis B virus / Q000235:genetics*; D022781:Hepatocytes / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology* / Q000821:virology; D014162:Transfection; D014407:Tumor Cells, Cultured