[Effect of liposome on permeability and drug retention of sodium fluorescein in rat skin: an experimental study].
OBJECTIVE
To investigate the effect of liposome on the permeability and drug retention of sodium fluorescein(NaFl) in rat skin.


METHODS
With an improved Franz diffusion cell and 0.125% NaFl liposome suspension as the model drug, in vitro measurement of percutaneous absorption and skin reservoir capacity for NaF1 was conducted using a fluorescence spectrophotometry at 4 and 12 h respectively after the diffusion experiment had started, and distribution of the fluorescence in rat skin was observed under fluorescence microscope at 4 h penetration experiment. NaFl solution of the same concentration as the model drugs served as the control for this experiment.


RESULTS
In comparison with NaFl solution, liposomal NaFl suspension resulted in larger amount of NaFl retention in rat skin but smaller amount in the receiver cell in a four-hour Franz diffusion test, with also higher fluorescence intensity in the skin, especially the skin around the hair follicles.


CONCLUSIONS
Local high drug concentrations can be achieved in the skin by liposomal suspension of water soluble drug for more effective treatment of skin diseases.
Xie
Yu-sheng
YS
Department of Dermatology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. shengyu@sohu.com
Zeng
Kang
K
Li
Guo-feng
GF
Dong
Ping
P
Shi
Yi-min
YM
Zhu
Xiao-liang
XL
Sun
Le-dong
LD
Hou
Gui-rong
GR
D000279:Administration, Cutaneous; D000818:Animals; D003287:Contrast Media / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D004337:Drug Carriers; D016503:Drug Delivery Systems; D019793:Fluorescein / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D008081:Liposomes / Q000737:chemistry*; D010539:Permeability; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption
D003287:Contrast Media; D004337:Drug Carriers; D008081:Liposomes; D019793:Fluorescein