title: [The Application of RACE Technique to Clone the Full-Length cDNA of A Novel Leukemia Associated Gene LRP16]
abstract: LRP16 is a novel gene which was found in our laboratory by using methylation-sensitive restriction landmark genomic scanning (RLGS) technique. In order to clone the full-length cDNA of this leukemia relapse associated gene, the method of rapid amplification of cDNA end (RACE) was employed. By optimizing some procedures of RACE method, the 5'- and 3'-untranslated region of LRP16 cDNA was successfully sequenced. Then, the full length of LRP16 cDNA and open reading frame (ORF) was constructed and was registered in GenBank. The above-mentioned procedure demonstrated RACE technique is a rapid and sensitive method for cloning unknown gene. Especially, it is very useful to cloning the 5'- and 3'-untranslated region of a novel gene.
authors.affiliation: Department of Hematology, The Genral Hospital of PLA, Beijing 100853, China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Han
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Fang-Ding
authors.initials: FD
authors.lastname: Lou
authors.forename: Quan-Shun
authors.initials: QS
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Zi-Jiang
authors.initials: ZJ
authors.lastname: Shi
authors.forename: Hai-Jie
authors.initials: HJ
authors.lastname: Jin