Effect of alternaria pathotoxin(s) on expression of p53-like apoptotic protein in calli and leaves of Brassica campestris.
Possible involvement of apoptosis was investigated in pathotoxin-treated and nutritionally-depleted in vitro cultured calli by comparing levels of p53-like protein. Antibodies raised against human p53 were used to detect and quantify p53 in B. campestris. Expression of p53-like protein increased from proliferating to static growth stage and reached to constant level at decaying stage. Both ELISA and dot immuno-binding assay showed that p53-like protein was over expressed in toxin treated and nutritionally depleted calli. Almost similar changes were seen in senescent damage in Brassica species indicating involvement of p53 dependent pathways.
Khandelwal
Abha
A
Department of Molecular Biology and Genetic Engineering, GB Pant University of Agriculture and Technology, Pantnagar 263 145, India.
Kumar
Anil
A
Banerjee
Manish
M
Garg
G K
GK
D000528:Alternaria; D017209:Apoptosis; D001937:Brassica / Q000187:drug effects* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D047630:Depsipeptides; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D066298:In Vitro Techniques; D009183:Mycotoxins / Q000494:pharmacology*; D010456:Peptides, Cyclic / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects* / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D047630:Depsipeptides; D009183:Mycotoxins; D010456:Peptides, Cyclic; D010940:Plant Proteins; D016159:Tumor Suppressor Protein p53; C040424:destruxin B