Cloning and sequencing of beta toxin gene of Clostridium perfringens type C.
A gene encoding beta toxin was amplified by polymerase chain reaction from C. perfringens type C isolate and cloned in pUC 19 vector. The nucleotide sequence was identical with C. perfringens type B beta toxin gene sequence. The Southern hybridization using labelled beta toxin gene probe revealed the presence of positive signals only in beta producing C. perfingens.
Goswami
P P
PP
National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar 243 122, India.
Girish
K S
KS
Chaudhuri
P
P
Tiwari
V
V
Akare
S J
SJ
Harbola
P C
PC
D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003016:Clostridium perfringens / Q000145:classification / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D001427:Bacterial Toxins; C031884:CPB protein, Clostridium perfringens; D017931:DNA Primers; D004269:DNA, Bacterial