title: [Construction of DNA vaccine including a chimeric gene encoding flagellin and outer membrane protein antigen from Leptospira interrogons serovar lai].
abstract: OBJECTIVE
  To construct a fusion expression vector for DNA vaccine, including a flagellin gene (flaB2) and an outer membrane protein gene (ompL1) to enhance the protective immunity of Leptospira interrogans serova Lai (Stain 017).
  
  
  METHODS
  The DNA fragments encoding flaB2 and ompL1 were amplified respectively by PCR and then fused. The obtained chimeric gene ompL1-flaB2 contained a synthetic linker of ten amino acids to sustain its space conformation.
  
  
  RESULTS
  Identified by restriction enzyme analysis, an insert fragment of 1.8 kb was demonstrated. FlaB2 and ompL1 respectively had the same sequence as reported by DNA sequencing analysis.
  
  
  CONCLUSIONS
  A fusion expression plasmid containing flaB2 and ompL1 was constructed.
authors.affiliation: Research Unit of Leptospirosis, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Baomin
authors.initials: B
authors.lastname: Dai
authors.forename: Zill
authors.initials: Z
authors.lastname: You
authors.forename: Zhimao
authors.initials: Z
authors.lastname: Fang
authors.forename: Yajing
authors.initials: Y
authors.lastname: Wang
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D005408:Flagellin / Q000235:genetics / Q000276:immunology*; D007921:Leptospira interrogans / Q000235:genetics* / Q000276:immunology; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D019444:Vaccines, DNA / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D005408:Flagellin