[Study on cadmium-induced hepatocellular apoptosis in rats].
The effect of cadmium chloride on hepatocellular apoptosis in rats was studied in vivo at the doses of 5, 10 and 20 mumol/kg by using TUNEL (TdT-mediated dUTP Nick End Labelling), DNA ladder electrophoresis and flow cytometry. The results showed that TUNEL-positive cells could be detected in cadmium-treated rat liver cells. DNA fragmentation (ladder pattern) in cadmium-treated liver cells could be displayed by agarose gel electrophoresis. The rates of apoptotic cells in the liver of cadmium-treated rats at these doses were higher than those of control rats significantly. It was suggested that cadmium chloride could induce hepatocellular apoptosis of rats at certain doses in vivo.
Yu
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RA
Tongji Medical College, Huazhong University of Sciences and Technology, Wuhan 430030, China.
Chen
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X
Lu
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D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D019256:Cadmium Chloride / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000473:pathology*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D019256:Cadmium Chloride