title: Systematic discovery of new genes in the Saccharomyces cerevisiae genome.
abstract: We used genome-wide comparative analysis of predicted protein sequences to identify many novel small genes, named smORFs for small open reading frames, within the budding yeast genome. Further analysis of 117 of these new genes showed that 84 are transcribed. We extended our analysis of one smORF conserved from yeast to human. This investigation provides an updated and comprehensive annotation of the yeast genome, validates additional concepts in the study of genomes in silico, and increases the expected numbers of coding sequences in a genome with the corresponding impact on future functional genomics and proteomics studies.
authors.affiliation: Genome Therapeutics Corporation, Waltham, Massachusetts 02453, USA.
authors.forename: Marco M
authors.initials: MM
authors.lastname: Kessler
authors.forename: Qiandong
authors.initials: Q
authors.lastname: Zeng
authors.forename: Sarah
authors.initials: S
authors.lastname: Hogan
authors.forename: Robin
authors.initials: R
authors.lastname: Cook
authors.forename: Arturo J
authors.initials: AJ
authors.lastname: Morales
authors.forename: Guillaume
authors.initials: G
authors.lastname: Cottarel
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D002417:Cattle; D002874:Chromosome Mapping / Q000379:methods; D015825:Chromosomes, Fungal / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D016681:Genome, Fungal; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011506:Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D029742:Caenorhabditis elegans Proteins; D029721:Drosophila Proteins; D011506:Proteins