title: [The expression of glucocorticold receptor isoform in peripheral blood mononuclear cell with Graves' ophthalmopathy].
abstract: OBJECTIVE
  To study the expression of glucocorticoid receptor (GR) isoform in the peripheral blood mononuclear cell (PBMC) of patients with Graves' ophthalmopathy (GO).
  
  
  METHODS
  Semi-quantitative RT-PCR was used to analyze RNA isolated from PBMC of the patients with GO and the normal volunteers. The level of plasma total cortisol (PTC) at 8 a.m. was measured.
  
  
  RESULTS
  The hGR alpha/GR beta mRNA ratios of the two groups were 7.58 +/- 5.42 and 14.65 +/- 8.30, respectively. There was significant difference between the two groups (P < 0.05). The PTC levels of the two groups were 304.23 +/- 92.06 and 313.73 +/- 111.05, and no significant difference between them was noted (P > 0.05). No correlation was found between hGR alpha/GR beta mRNA and PTC levels.
  
  
  CONCLUSIONS
  hGR alpha/GR beta mRNA may play a role in the pathogeny of Graves' ophthalmopathy.
authors.affiliation: Department of Endocrinology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Zhenmei
authors.initials: Z
authors.lastname: An
authors.forename: Nan
authors.initials: N
authors.lastname: Yu
authors.forename: Songquan
authors.initials: S
authors.lastname: Wei
authors.forename: Qingjie
authors.initials: Q
authors.lastname: Xia
authors.forename: Shuangqing
authors.initials: S
authors.lastname: Li
authors.forename: Qin
authors.initials: Q
authors.lastname: Xie
mesh_terms: D000328:Adult; D005260:Female; D006111:Graves Disease / Q000097:blood*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D020033:Protein Isoforms / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D006854:Hydrocortisone