[Metabolic regulation of phenylethanoid glycosides from Herba cistanches in dogs' gastrointestine].
OBJECTIVE
To study the metabolic process of phenylethanoid glycosides (PhGs) in the gastrointestine of beagle dogs that were administered intragastrially process, and develop some new methods of biopharmacology on the effective position of traditional Chinese medicine.


METHODS
High-performance liquid chromatography was used to purify constituents from faeces and analyze relative contents of the three main compounds in the gastrointestinal tract at different times and in the faeces of dogs. Every sample was collected, extracted with methanol and analyzed with integration.


RESULTS
Four compounds, based on reference substances, were identified as echinacoside, acteoside, isoacteoside, and 2'-acetylacteoside from extraction of faces of dogs. Quantitative "with HPLC" analysis reveals that variation of ratios of the three main compounds is not distinct when moving in the gastrointestinal tract 7 h, that is quite different from those in faeces, in which the content of echinacoside fell from 48.0% to 16.0%, and acteoside increased from 11.0% to 34.7%.


CONCLUSIONS
PhGs are mainly metabolized in large intestine. Among them, a portion of echinacoside is transformed into aceteoside.
Lei
L
L
Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110015, China.
Song
Z H
ZH
Tu
P F
PF
Li
Y Z
YZ
Wu
L J
LJ
Chen
F K
FK
D000818:Animals; D031671:Cistanche / Q000737:chemistry; D004285:Dogs; D005243:Feces / Q000737:chemistry; D005753:Gastric Mucosa / Q000378:metabolism; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007420:Intestine, Large / Q000378:metabolism*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification; D010626:Phenylethyl Alcohol / Q000302:isolation & purification / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D059808:Polyphenols
D005960:Glucosides; D006027:Glycosides; D010636:Phenols; D010626:Phenylethyl Alcohol; C058956:acteoside; D059808:Polyphenols; C060297:echinacoside