Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate.
(-)-Epigallocatechin gallate (EGCG), abundant in green tea, is a potent anti-microbial and anti-tumor compound. This investigation used immunoblot, flow cytometry, microarray, and indirect immunofluorescence analyses to show that at concentrations exceeding 50 microM, EGCG inhibits the expression of Epstein-Barr virus (EBV) lytic proteins, including Rta, Zta, and EA-D, but does not affect the expression of EBNA-1. Moreover, DNA microarray and transient transfection analyses demonstrated that EGCG blocks EBV lytic cycle by inhibiting the transcription of immediate-early genes, thus inhibiting the initiation of EBV lytic cascade.
Chang
Li-Kwan
LK
Department of Biology, Kaohsiung Medical University, 100, Shih-Chuan 1st Rd., 807, Kaohsiung, Taiwan.
Wei
Ta-Tung
TT
Chiu
Ya-Fang
YF
Tung
Chao-Ping
CP
Chuang
Jian-Ying
JY
Hung
Shang-Kai
SK
Li
Ching
C
Liu
Shih-Tung
ST
D000998:Antiviral Agents / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000096:biosynthesis / Q000235:genetics; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D017781:Genes, Immediate-Early / Q000187:drug effects; D004854:Herpesvirus 4, Human / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
D000998:Antiviral Agents; D019309:Epstein-Barr Virus Nuclear Antigens; D014764:Viral Proteins; D002392:Catechin; C045651:epigallocatechin gallate; C417029:EBV-encoded nuclear antigen 1