The Arabidopsis lue1 mutant defines a katanin p60 ortholog involved in hormonal control of microtubule orientation during cell growth.
The lue1 mutant was previously isolated in a bio-imaging screen for Arabidopsis mutants exhibiting inappropriate regulation of an AtGA20ox1 promoter-luciferase reporter fusion. Here we show that lue1 is allelic to fra2, bot1 and erh3, and encodes a truncated katanin-like microtubule-severing protein (AtKSS). Complementation of lue1 with the wild-type AtKSS gene restored both wild-type stature and luciferase reporter levels. Hormonal responses of lue1 to ethylene and gibberellins revealed inappropriate cortical microtubule reorientation during cell growth. Moreover, a fusion between the AtKSS protein and GFP decorated cortical microtubules. A yeast two-hybrid screen with AtKSS as the bait identified proteins related to those involved in microtubule processing, including a katanin p80 subunit and a kinesin ortholog. These results indicate that AtKSS is involved in microtubule dynamics in response to plant hormones.
Bouquin
Thomas
T
Institute of Molecular Biology, Department of Plant Physiology, Øster Farimagsgade 2A, 1353K Copenhagen, Denmark.
Mattsson
Ole
O
Naested
Henrik
H
Foster
Randy
R
Mundy
John
J
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000502:physiology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017930:Genes, Reporter / Q000235:genetics; D005875:Gibberellins / Q000494:pharmacology; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D000074201:Katanin; D020894:Microfibrils / Q000187:drug effects / Q000378:metabolism; D008859:Microscopy, Polarization; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D029681:Arabidopsis Proteins; D005030:Ethylenes; D005875:Gibberellins; D010937:Plant Growth Regulators; D012333:RNA, Messenger; C036216:ethylene; D005966:Glucuronidase; D000251:Adenosine Triphosphatases; C436244:KTN1 protein, Arabidopsis; D000074201:Katanin