Role of alcohol and genetic polymorphisms of CYP2E1 and ALDH2 in breast cancer development.
OBJECTIVE
We examined the potential association between alcohol consumption and genetic polymorphisms in the alcohol metabolizing enzymes, CYP2E1 and ALDH2, in individual susceptibility to breast cancer in a Korean study population.


METHODS
Three hundred and forty-six histologically confirmed breast cancer patients and 377 controls with no present or previous history of cancer were recruited from several teaching hospitals in Seoul during 1995-2001. The CYP2E1 RsaI polymorphism was determined by a real time PCR method, and the ALDH2 Glu487 Lys polymorphism was determined by a PCR method with confronting two-pair primers (PCR-CTPP).


RESULTS
The drinking women had a 1.4-fold risk for breast cancer (95% CI = 0.99-2.11) compared to never drinkers after adjustment for age and family history of breast cancer. No statistically significant overall differences were seen in the genotype frequencies between breast cancer cases and controls. However, the "ever"-drinking women with the CYP2E1 c2 allele containing genotypes had a 1.9-fold risk (95% CI = 0.99-3.83) for developing breast cancer compared to non-drinkers with the CYP2E1 c1/c1 genotype (P for interaction = 0.043).


CONCLUSIONS
This study therefore suggests that the CYP2E1 c2 allele may influence the individual susceptibility to breast cancer in alcohol-consuming women.
Choi
Ji-Yeob
JY
Department of Preventive Medicine, General Surgery and Cancer Research Institute, Seoul National University College of Medicine, 27 Yongon-Dong, Chongno-Gu, Seoul 110-799, Korea.
Abel
Josef
J
Neuhaus
Thomas
T
Ko
Yon
Y
Harth
Volker
V
Hamajima
Nobuyuki
N
Tajima
Kazuo
K
Yoo
Keun-Young
KY
Park
Sue Kyung
SK
Noh
Dong-Young
DY
Han
Wonshik
W
Choe
Kuk-Jin
KJ
Ahn
Sei-Hyun
SH
Kim
Sook-Un
SU
Hirvonen
Ari
A
Kang
Daehee
D
D000328:Adult; D000428:Alcohol Drinking / Q000235:genetics* / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D000483:Alleles; D001943:Breast Neoplasms / Q000201:enzymology / Q000235:genetics*; D016022:Case-Control Studies; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D018450:Disease Progression; D000431:Ethanol / Q000378:metabolism; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015183:Restriction Mapping; D012307:Risk Factors
D017931:DNA Primers; D000431:Ethanol; D019392:Cytochrome P-450 CYP2E1; C096127:ALDH2 protein, human; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial