Role of calcium in E-selectin induced phenotype of T84 colon carcinoma cells.
The adhesion of cancer cells to the endothelium during the metastatic process involves the interaction of specific cell-cell adhesion receptors on the cell surface. E-selectin on endothelial cells and sialyl Lewis X carbohydrate component on tumor cells are mainly implicated in the adhesion of colon carcinoma cells to the endothelium of target organ. In this paper we show that binding of E-selectin to T84 colon tumor cells causes approximately a twofold increase in intracellular calcium concentration. In particular, using two inhibitors of receptor operated calcium channels, CAI and SK&F 96365, we present evidences that the augmentation in cytoplasmic calcium originates from ionic influx from extracellular sources. Furthermore, we demonstrated that modulation of [Ca2+]i by engagement of E-selectin receptor starts signal transduction pathways that affect cell spreading, tyrosine phosphorylation signaling, and cancer cell motility.
D'Amato
M
M
Dipartimento di Biopatologia e Metodologie Biomediche, Università di Palermo, Via Divisi 83, 90133, Palermo, Italy.
Flugy
A M
AM
Alaimo
G
G
Bauder
B
B
Kohn
E C
EC
De Leo
G
G
Alessandro
R
R
D000970:Antineoplastic Agents / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D019040:E-Selectin / Q000494:pharmacology*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D010641:Phenotype; D010766:Phosphorylation; D011994:Recombinant Proteins / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000378:metabolism
D000970:Antineoplastic Agents; D002121:Calcium Channel Blockers; D019040:E-Selectin; D007093:Imidazoles; D011994:Recombinant Proteins; D014230:Triazoles; D014443:Tyrosine; C062058:carboxyamido-triazole; C063159:1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole; D002118:Calcium