HRMAS NMR observation of beta-sheet secondary structure on a water swollen solid support.
In this paper HRMAS NMR was used to investigate whether peptides on a peptidyl resin swollen in aqueous solution can adopt an intramolecular beta-sheet structure. A model peptide YQNPDGSQA, that was previously shown to adopt such a secondary structure in solution, (Blanco et al, J. Am. Chem. Soc., 1993) was grafted onto three different solid supports that swell in aqueous solution to examine the influence of the resin on the structure. Both parameters of resin loading and pH inside the swollen peptidyl resin proved to be important for the physicochemical behaviour of the peptide on the support.
Rousselot-Pailley
Pierre
P
UMR 8525 CNRS-Institut de Biologie de Lille-Université de Lille II and Institut Pasteur de Lille, 1 rue du Professeur Calmette, BP447, 59021 Lille Cedex, France.
Boutillon
Christophe
C
Wieruszeski
Jean-Michel
JM
Lippens
Guy
G
D009682:Magnetic Resonance Spectroscopy; D010455:Peptides / Q000737:chemistry*; D017433:Protein Structure, Secondary; D014867:Water / Q000737:chemistry
D010455:Peptides; D014867:Water