title: Upregulation of purinergic P2Y receptor-mediated calcium responses in glial cells during experimental detachment of the rabbit retina.
abstract: To investigate injury-induced alterations of purinergic P2Y receptor-mediated calcium responses in glial (Müller) cells of the rabbit retina, neural retinae were experimentally detached from the pigment epithelium. The ATP-evoked calcium responses were recorded in the endfeet of glial cells at the vitread surface of retinal wholemounts. In control retinae, approximately 7% of the glial cells investigated showed ATP-evoked calcium responses. Within 24 h of detachment, significantly more retinal glial cells (42%) showed calcium responses, and glial ATP responsiveness increased further in retinae which were detached for 48 (44%) or for 72 h (64%). The results indicate that in the detached retina, glial cells upregulate their responsiveness to extracellular ATP within 24 h of injury. Thus, P2Y receptor-mediated signalling may be involved in the early steps of glial response to retinal injury.
authors.affiliation: Department of Neurophysiology, Paul Flechsig Institute of Brain Research, University of Leipzig, D-04109, Leipzig, Germany.
authors.forename: Ortrud
authors.initials: O
authors.lastname: Uckermann
authors.forename: Susann
authors.initials: S
authors.lastname: Uhlmann
authors.forename: Michael
authors.initials: M
authors.lastname: Weick
authors.forename: Thomas
authors.initials: T
authors.lastname: Pannicke
authors.forename: Mike
authors.initials: M
authors.lastname: Francke
authors.forename: Andreas
authors.initials: A
authors.lastname: Reichenbach
authors.forename: Peter
authors.initials: P
authors.lastname: Wiedemann
authors.forename: Andreas
authors.initials: A
authors.lastname: Bringmann
mesh_terms: D000818:Animals; D020013:Calcium Signaling; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D018613:Microscopy, Confocal; D009457:Neuroglia / Q000378:metabolism* / Q000473:pathology; D011817:Rabbits; D018048:Receptors, Purinergic P2 / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*; D012163:Retinal Detachment / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation
chemical_list: D018048:Receptors, Purinergic P2