title: [Analysis of the diterpenoids in the extract of Pteris semipinnata L by HPLC-APCI-MS].
abstract: OBJECTIVE
  To establish an accurate and reliable method for quantitative analysis of the diterpenoids in Pteris semipinnata L.
  
  
  METHODS
  A quadruple mass spectrometer coupled with atmospheric pressure chemical ionization interface was employed as a detector for HPLC. As to MS detector, selective ion monitoring (SIM) scan mode was used. For ent-11 alpha-hydroxy-15-oxo-kaur-16-en-19-olic acid (5F) and ent-11 alpha-hydroxy-15-oxo-kaur-16(R) methyl-19-olic acid (4F), the majority of the diterpenoids in Pteris semipinnata L, the [M-H]-1 ion were observed, and the [M-H2O-H]-1 ion could be observed from the collision-induced dissociation spectua. [M-H]-1 was selected as the SIM ion in quantification, the mobile phase and the MS conditions were optimized. The mobile phase of HPLC was 30% CH3CN-70% 2 mmol.L-1 NH4Ac, analytical column was Diamonsil ODS (4.6 mm x 150 mm), flow rate 1.0 mL.min-1, inject volume 5 microL. The area of ion flow peak were used for quantitative determination. As an example of its application, this method was used to determine the content of 5F as an antitumor diterpenoid in Pteris semipinnata L.
  
  
  RESULTS
  The content of 5F accounted 1.18 mg.g-1 in Pteris semipinnata L sample. For 5F, RT is about 4.3 min, the standard curve showed good linearity over the range of 0.05-2.5 micrograms, gamma = 0.9998 (n = 5); the recovery was 97.8% (n = 5); the limit of detection was 0.4 ng (inject 5 microL).
  
  
  CONCLUSIONS
  This method is highly sensitive, accurate and fast, which can be applied to study the antitumor drug of diterpenoids in Pteris semipinnata L and to establish the raw herb standard.
authors.affiliation: Guangdong Medical College, Key Laboratory of Natural Drugs Research and Development of Guangdong, Zhanjiang 524023, China. dengfy@gdmc.edu.cn
authors.forename: Yi-feng
authors.initials: YF
authors.lastname: Deng
authors.forename: Nian-ci
authors.initials: NC
authors.lastname: Liang
authors.forename: Tong
authors.initials: T
authors.lastname: Liang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004224:Diterpenes / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D032495:Pteris / Q000737:chemistry*; D011786:Quality Control
chemical_list: C479310:11-hydroxy-15-oxokaur-16-en-19-olic acid; D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes