Prion peptide induces neuronal cell death through a pathway involving glycogen synthase kinase 3.
Prion diseases are characterized by neuronal cell death, glial proliferation and deposition of prion peptide aggregates. An abnormal misfolded isoform of the prion protein (PrP) is considered to be responsible for this neurodegeneration. The PrP 106-126, a synthetic peptide obtained from the amyloidogenic region of the PrP, constitutes a model system to study prion-induced neurodegeneration as it retains the ability to trigger cell death in neuronal cultures. In the present study, we show that the addition of this prion peptide to cultured neurons increases the activity of glycogen synthase kinase 3 (GSK-3), which is accompanied by the enhanced phosphorylation of some microtubule-associated proteins including tau and microtubule-associated protein 2. Prion peptide-treated neurons become progressively atrophic, and die ultimately. Both lithium and insulin, which inhibit GSK-3 activity, significantly decrease prion peptide-induced cell death both in primary neuronal cultures and in neuroblastoma cells. Finally, the overexpression of a dominant-negative mutant of GSK-3 in transfected neuroblastoma cells efficiently prevents prion peptide-induced cell death. These results are consistent with the view that the activation of GSK-3 is a crucial mediator of prion peptide-induced neurodegeneration.
Pérez
Mar
M
Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma Madrid, 28049 Madrid, Spain.
Rojo
Ana I
AI
Wandosell
Francisco
F
Díaz-Nido
Javier
J
Avila
Jesús
J
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D004789:Enzyme Activation / Q000502:physiology; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D010446:Peptide Fragments / Q000378:metabolism*; D011328:Prions / Q000378:metabolism*
D007004:Hypoglycemic Agents; D007328:Insulin; D010446:Peptide Fragments; D011328:Prions; C082632:prion protein (106-126); D038362:Glycogen Synthase Kinase 3