[Research on preparation of mycobacterium tuberculosis DNA microarray].
OBJECTIVE
To optimize and develop the technique for mycobacterium tuberculosis DNA microarray.


METHODS
The process included preparation of DNA samples, spotting and past-spotting treatment of arrays. DNA microarrays were prepared by spotting fluorescence labeled PCR products of target genes onto specially treated glass slides with robotics. The fluorescent signals before and after treatment were scanned with a scanner, and the DNA attachment rate was calculated from the obtained data by software.


RESULTS
A foundation for optimizing the conditions of Mycobacterium tuberculosis DNA microarrays has been laid. The support aldehyde-modified glass slide is useful for anchoring DNA at Some distance. DMSO as spotting solution is of benefit to preparation of Mycobacterium tuberculosis DNA microarray. Drying the chip at 37 degrees C temperature after spotting can enhance the DNA combination rate.


CONCLUSIONS
Several key steps of this technique have been optimized. This study has provided a foundation for optimizing the DNA attachment conditions in creating mycobacterium tuberculosis DNA microarray.
Zhang
Wanjiang
W
Research Unit of Infection and Immunity, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Bao
Lang
L
Wang
Xiaoying
X
Xie
Yong'en
Y
Chen
Wei
W
Zhang
Huidong
H
Yu
Xianghua
X
D004269:DNA, Bacterial / Q000032:analysis; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis
D004269:DNA, Bacterial