[Experimental study on inhibitory effect of rutin against platelet activation induced by platelet activating factor in rabbits].
OBJECTIVE
To explore the inhibitory effect and mechanism of rutin against platelet activating factor (PAF) induced platelet aggregation, 5-HT release and intra-platelet free calcium concentration.


METHODS
The rate of washed rabbit platelet (WRP) aggregation was measured by turbidimetry and O-phthaldialdehyde (OPT) fluoro-spectrophotometry (FSPM) was used to determine 5-HT content. The intraplatelet free calcium concentration was measured with Fura-2/AM FSPM assay.


RESULTS
Rutin in vitro was concentration-dependently inhibiting PAF (9.55 x 10(-9) mol/L) induced WRP aggregation, the IC50 of 5-HT release was 0.73, 1.13 mmol/L respectively and the intraplatelet free calcium concentration elevation evoked by PAF (4.78 x 10(-10) mol/L) were inhibited by 68.3, 136, 274, 545 mumol/L of rutin dose-dependently.


CONCLUSIONS
Rutin could inhibit PAF induced platelet aggregation, 5-HT release and the increase of intraplatelet free calcium.
Chen
Wen-mei
WM
Beijing Institute of Heart, Lung and Vascular Diseases, Beijing 100029.
Jin
Ming
M
Wu
Wei
W
D000818:Animals; D001693:Biological Transport, Active; D001792:Blood Platelets / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D008297:Male; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D015539:Platelet Activation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011817:Rabbits; D012431:Rutin / Q000494:pharmacology*; D012701:Serotonin / Q000378:metabolism
D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D012701:Serotonin; D012431:Rutin; D002118:Calcium