[Cloning and sequence analysis of 55 K protein of egg drop syndrome virus].
OBJECTIVE
For understanding the characteristics of genomic structure of egg drop syndrome virus(EDSV).


METHODS
Nucleic acid was extracted using routine method from weak virulent strain AA-2 of EDSV isolated from Chinese sick hens. Construction of the whole genomic library was by hydrolysis with Hind III, strand encoding 55 K gene locating in Hind III--A segment was sequenced and analyzed.


RESULTS
The open reading frame has a length of 1,014 nt and codes a polypeptide of 337 amino acids with molecular weight of 38,200. Analysis of the amino acid sequence revealed a homology from 25.5%-32.4% to the 55 K protein of human adenovirus types 2, 12, 40, canine adenovirus and fowl adenoviruses of group 1, whereas to ovine adenovirus is 46.4%.


CONCLUSIONS
The genomic structure of EDSV has some relationship with adenoviruses.
Zhu
L
L
State Key Laboratory of Molecular Virology and Genetic Engineering, CAMP, Beijing 100052.
Jin
Q
Q
Zeng
L
L
D000818:Animals; D000259:Aviadenovirus / Q000235:genetics*; D001483:Base Sequence; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
D014157:Transcription Factors; D014764:Viral Proteins; C056461:terminal protein, adenovirus