title: Effect of alternaria pathotoxin(s) on expression of p53-like apoptotic protein in calli and leaves of Brassica campestris.
abstract: Possible involvement of apoptosis was investigated in pathotoxin-treated and nutritionally-depleted in vitro cultured calli by comparing levels of p53-like protein. Antibodies raised against human p53 were used to detect and quantify p53 in B. campestris. Expression of p53-like protein increased from proliferating to static growth stage and reached to constant level at decaying stage. Both ELISA and dot immuno-binding assay showed that p53-like protein was over expressed in toxin treated and nutritionally depleted calli. Almost similar changes were seen in senescent damage in Brassica species indicating involvement of p53 dependent pathways.
authors.affiliation: Department of Molecular Biology and Genetic Engineering, GB Pant University of Agriculture and Technology, Pantnagar 263 145, India.
authors.forename: Abha
authors.initials: A
authors.lastname: Khandelwal
authors.forename: Anil
authors.initials: A
authors.lastname: Kumar
authors.forename: Manish
authors.initials: M
authors.lastname: Banerjee
authors.forename: G K
authors.initials: GK
authors.lastname: Garg
mesh_terms: D000528:Alternaria; D017209:Apoptosis; D001937:Brassica / Q000187:drug effects* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D047630:Depsipeptides; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D066298:In Vitro Techniques; D009183:Mycotoxins / Q000494:pharmacology*; D010456:Peptides, Cyclic / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects* / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D047630:Depsipeptides; D009183:Mycotoxins; D010456:Peptides, Cyclic; D010940:Plant Proteins; D016159:Tumor Suppressor Protein p53; C040424:destruxin B