Applications of calorimetric methods to drug discovery and the study of protein interactions.
Recent studies report the application of isothermal titration calorimetry and differential scanning calorimetry to the study of protein-ligand interactions, allosteric cooperativity and aspects of protein folding. New methods of data analysis compare alternative methods for determining ligand binding enthalpy and analyze potential sources of error in the experimental measurement of other thermodynamic parameters. Several reports examine issues concerning drug design and the correlation of thermodynamic and X-ray structural data. New instruments allow volumetric effects in biochemical systems to be evaluated calorimetrically and to substantially expand the throughput of differential scanning calorimetry measurements in drug discovery and other high-throughput applications.
Weber
Patricia C
PC
3-Dimensional Pharmaceuticals Inc, 1020 Stony Hill Road, Yardley, PA 19067, USA.
Salemme
F Raymond
FR
D002151:Calorimetry / Q000295:instrumentation* / Q000379:methods*; D002152:Calorimetry, Differential Scanning / Q000295:instrumentation / Q000379:methods; D015195:Drug Design; D004735:Energy Transfer; D019277:Entropy; D008024:Ligands; D046911:Macromolecular Substances; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D013696:Temperature
D008024:Ligands; D046911:Macromolecular Substances; D011506:Proteins