Mosaicism of Solid Gold supports the causality of a noncoding A-to-G transition in the determinism of the callipyge phenotype.
To identify the callipyge mutation, we have resequenced 184 kb spanning the DLK1-, GTL2-, PEG11-, and MEG8-imprinted domain and have identified an A-to-G transition in a highly conserved dodecamer motif between DLK1 and GTL2. This was the only difference found between the callipyge (CLPG) allele and a phylogenetically closely related wild-type allele. We report that this SNP is in perfect association with the callipyge genotype. The demonstration that Solid Gold-the alleged founder ram of the callipyge flock-is mosaic for this SNP virtually proves the causality of this SNP in the determinism of the callipyge phenotype.
Smit
Maria
M
Department of Animal, Dairy and Veterinary Sciences, College of Agriculture, Utah State University, Logan, Utah 84322-4700, USA.
Segers
Karin
K
Carrascosa
Laura Garcia
LG
Shay
Tracy
T
Baraldi
Francesca
F
Gyapay
Gabor
G
Snowder
Gary
G
Georges
Michel
M
Cockett
Noelle
N
Charlier
Carole
C
D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D009030:Mosaicism / Q000235:genetics*; D009135:Muscular Diseases / Q000235:genetics*; D017354:Point Mutation; D012756:Sheep / Q000002:abnormalities / Q000235:genetics*