[The preliminary experiment of gene transfer into cultured rat retinal neural cells].
OBJECTIVE
To investigate the possibility of gene transfer into retina neural cells.


METHODS
pcDNA3-LacZ and pcDNA3-GDNF were transferred into rat retinal neural cells with cationic lipofectin. The gene transfer and expression of GDNF were detected by means of RT-PCR and SDS-PAGE.


RESULTS
pcDNA3-LacZ and pcDNA3-GDNF could be transferred into retinal neural cells and the expressive product of GDNF gene, a 32 KD protein band, was detected.


CONCLUSIONS
The preliminary results suggest that LacZ gene could be a marker to identify gene transfer into the target tissue and cells; GDNF gene could be transferred into cultured retinal neural cells of SD rats, and express 32 KD protein.
Ge
J
J
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Li
Y
Y
Zhuo
Y
Y
Guo
Y
Y
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D018014:Gene Transfer Techniques; D017930:Genes, Reporter / Q000235:genetics; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007763:Lac Operon / Q000235:genetics*; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D010714:Phosphatidylethanolamines; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000166:cytology* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D010714:Phosphatidylethanolamines; C020888:1,2-dielaidoylphosphatidylethanolamine