title: Development of a 5' fluorogenic nuclease-based real-time PCR assay for quantitative detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis.
abstract: A 5' nuclease TaqMan PCR was developed for the quantitative detection of the periodontopathic bacteria Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. The relative numbers of bacteria were measured by the comparative threshold cycle method. This simplified method is a way of obtaining the relative quantities of these organisms from specimens and of monitoring the effect of therapy.
authors.affiliation: Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yoshida
authors.forename: Nao
authors.initials: N
authors.lastname: Suzuki
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Nakano
authors.forename: Takahiko
authors.initials: T
authors.lastname: Oho
authors.forename: Miki
authors.initials: M
authors.lastname: Kawada
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Koga
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis; D003851:Deoxyribonucleases / Q000378:metabolism*; D006801:Humans; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D003851:Deoxyribonucleases