Tribulosin and beta-sitosterol-D-glucoside, the anthelmintic principles of Tribulus terrestris.
Successive extracts of Tribulus terrestris prepared using petroleum ether, chloroform, 50% methanol and water were tested for anthelmintic activity in-vitro using the nematode Caenorhabditis elegans. The activity could be detected only in 50% methanol extract which on further bioactivity guided fractionation and chromatographic separation yielded a spirostanol type saponin, tribulosin and beta-sitosterol-D-glucoside. Both the compounds exhibited anthelmintic activity with ED50 of 76.25 and 82.50 microg/ml respectively.
Deepak
M
M
Phytochemistry Laboratory, R&D Centre, Natural Remedies Pvt. Ltd., Veerasandra Indl. Area, Bangalore, India.
Dipankar
G
G
Prashanth
D
D
Asha
M K
MK
Amit
A
A
Venkataraman
B V
BV
D000818:Animals; D000969:Antinematodal Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D017173:Caenorhabditis elegans / Q000187:drug effects*; D006801:Humans; D007928:Lethal Dose 50; D009349:Nematode Infections / Q000188:drug therapy; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D012855:Sitosterols / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D032444:Tribulus
D000969:Antinematodal Agents; D010936:Plant Extracts; D012855:Sitosterols; C472188:tribulosin; C011015:lyoniside