Response of Penaeus indicus females at two different stages of ovarian development to a lethal infection with Vibrio penaeicida.
An association between vitellogenesis and the immune system was suggested in crustaceans from studies on plasma lipoproteins. The present research studies the effect of an experimentally induced bacterial infection on vitellogenesis in females of the shrimp Penaeus indicus, as a model for penaeid species. Pre-vitellogenic and vitellogenic P. indicus females were experimentally infected with an extremely pathogenic bacterium, Vibrio penaeicida. The peak in mortality occurred earlier in pre-vitellogenic animals than in vitellogenic ones, although the final mortality level ( approximately 64-74%) 52h post-infection was nearly the same for the two groups. Twenty hours after infection, the total number of haemocytes was significantly reduced in vitellogenic females while there was no change in the pre-vitellogenic group. Protein synthesis in ovaries was not significantly affected by infection, at the two stages of ovarian development. No differences were found in mRNA levels of shrimp ovarian peritrophin protein (SOP), but preliminary results showed that mRNA expression of vitellin (VT) was reduced in a heavily infected vitellogenic female. The total amount of lipids in the haemolymph of vitellogenic females was almost twice higher than that of pre-vitellogenic ones. However, there was no change in the total content of lipids, lipid classes and fatty acid distribution in haemolymph or hepatopancreas following infection. Although vitellogenic and pre-vitellogenic females probably respond differently to a lethal bacterial infection, physiological differences may be concealed by the rapid onset of mortality.
Avarre
J-C
JC
Department of Marine Biology, Israel Oceanographic and Limnological Research Ltd., Tel Shikmona, P.O. Box 8030, 31080, Haifa, Israel.
Saulnier
D
D
Labreuche
Y
Y
Ansquer
D
D
Tietz
A
A
Lubzens
Esther
E
D000818:Animals; D005260:Female; D010053:Ovary / Q000254:growth & development*; D033561:Penaeidae / Q000382:microbiology* / Q000502:physiology*; D014735:Vibrio Infections / Q000401:mortality / Q000503:physiopathology*; D014818:Vitellogenesis / Q000502:physiology*