[Cloning of staphylococcal enterotoxin B gene and its highly expression in Escherichia coli].
An about 700 bp DNA fragment was amplified from genome DNA of S. aureus TSTw by PCR. This fragment was cloned into pGEM-7Zf(+) and the recombinant plasmid was transformed into E. coli DH5 alpha. The sequencing result of the recombinant plasmid demonstrated that it contains seb gene with 717 bp (without signal encoding region of 81 bp) which has the same nucleotide sequence as described in literature. The seb gene was cloned into expression vector 7ZTS and was transformed into E. coli JM109 (DE3). The expression level of SEB was as high as 33.3% of the cell total proteins.
Yang
Li-Quan
LQ
Shenyang Institute of Applied Ecology, Chinese Academy of Science, Shenyang 110015, China.
Wu
Wen-Fang
WF
Shi
Cheng-Bo
CB
Lu
An-Guo
AG
Feng
Jia-Xun
JX
Bai
Xue-Liang
XL
D003001:Cloning, Molecular; D004768:Enterotoxins / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005818:Genetic Engineering; D011994:Recombinant Proteins / Q000096:biosynthesis*
D004768:Enterotoxins; D011994:Recombinant Proteins; C031818:enterotoxin B, staphylococcal