title: Identification of a functional domain within the essential core of histone H3 that is required for telomeric and HM silencing in Saccharomyces cerevisiae.
abstract: Fourteen novel single-amino-acid substitution mutations in histone H3 that disrupt telomeric silencing in Saccharomyces cerevisiae were identified, 10 of which are clustered within the alpha1 helix and L1 loop of the essential histone fold. Several of these mutations cause derepression of silent mating locus HML, and an additional subset cause partial loss of basal repression at the GAL1 promoter. Our results identify a new domain within the essential core of histone H3 that is required for heterochromatin-mediated silencing.
authors.affiliation: Department of Biology, Georgian Court College, Lakewood, New Jersey 08701, USA. thompsonj@georgian.edu
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Thompson
authors.forename: Marilyn L
authors.initials: ML
authors.lastname: Snow
authors.forename: Summer
authors.initials: S
authors.lastname: Giles
authors.forename: Leslie E
authors.initials: LE
authors.lastname: McPherson
authors.forename: Michael
authors.initials: M
authors.lastname: Grunstein
mesh_terms: D020868:Gene Silencing; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D016296:Mutagenesis; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D016615:Telomere / Q000378:metabolism*
chemical_list: D006657:Histones