[Isolation, purification and analysis of polysaccharides from Adenophora potaninii Korsh].
Polysaccharides, AP-1 and AP-3, were obtained through extraction of the roots of Adenophora potaninii Korsh by hot water, followed by the isolation and purification by DEAE-cellulose and Sephadex G-200 column chromatography. It was proved to be homogeneous by gel filtration chromatography and their average MW was estimated to be 8.3 x 10(4) and 6.3 x 10(4) respectively. Analysis of sugar composition showed that AP-1 is D-glucan, while AP-3 is composed of glucose, rhamnose, arabinose and glucronic acid in the molar ratio of 5:1:1:3.
Chen
Qian
Q
College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650092.
Chen
Xiaoming
X
Cai
Yuejun
Y
Wang
Fei
F
Li
Zhixiao
Z
D029422:Campanulaceae / Q000737:chemistry*; D002850:Chromatography, Gel; D005936:Glucans / Q000737:chemistry / Q000302:isolation & purification*; D005947:Glucose / Q000302:isolation & purification; D020723:Glucuronic Acid / Q000302:isolation & purification; D008970:Molecular Weight; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D012210:Rhamnose / Q000302:isolation & purification
D005936:Glucans; D011134:Polysaccharides; D020723:Glucuronic Acid; D005947:Glucose; D012210:Rhamnose