[Effect of yangjing zhongyu decoction on expression of insulin-like growth factor II and its receptor in endometrium of women with unexplained infertility].
OBJECTIVE
To investigate the effect of Yangjing Zhongyu Decoction (YJZYD) on expression of insulin-like growth factor II (IGF-II) and its receptor II (IGF-II R) in endometrium of women with unexplained infertility, and the relationship of which with the receptibility of endometrium to ovum implantation.


METHODS
Reverse transcription-polymerase chain reaction (RT-PCR) assay was used to detect quantitatively the expression of IGF-II and IGF-II R in 22 women with unexplained infertility before and after YJZYD treatment during mid-luteal phase.


RESULTS
The levels of IGF-II and IGF-II R before treatment were 0.794 +/- 0.453 and 0.725 +/- 0.354 (in grey level, the same below) respectively, which were significantly increased in the same phase after treatment, reaching 1.202 +/- 0.551 and 1.045 +/- 0.581 respectively (P < 0.01 and P < 0.05). Correlation analysis showed the level of IGF-II mRNA was positively correlated with the level of IGF-II mRNA either before or after treatment.


CONCLUSIONS
YJZYD could enhance the expression of IGF-II and IGF-II R in the endometrium during mid-luteal phase, promote the differentiation of endometrium and increase its reception to ovum implantation.
Wu
Rui-jin
RJ
Women's Hospital, Medical School of Zhejiang University, Hangzhou 310006.
Zhou
Fu-zhen
FZ
D000328:Adult; D010064:Embryo Implantation / Q000187:drug effects; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000188:drug therapy* / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis* / Q000235:genetics; D008183:Luteal Phase; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D017527:Receptor, IGF Type 2 / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D017527:Receptor, IGF Type 2; D007335:Insulin-Like Growth Factor II