Effect of C-peptide on wound healing and microcirculation in diabetic mice.
OBJECTIVE
Recent studies have demonstrated that C-peptide is biologically active and might have a beneficial effect on late complications in diabetes mellitus. The aim of this study was to investigate the effects of systemically given C-peptide on dermal wound healing in diabetic mice.


METHODS
Experiments were carried out in male SKH-1 hr hairless mice. Dermal wounds were created (diameter 2.5mm) in streptozotozin-diabetic and normal control mice. Mice were randomized into three treatment groups (n = 10 each): Normal controls, diabetic mice with PBS or C-peptide injection twice daily. At various time points (prior wounding as well as days 4, 7, 10 and 15) microcirculation was quantitatively analyzed by intravital fluorescent microscopy to determine wound surface area, vessel diameter, red blood cell velocity, plasma leakage, functional capillary density. In addition, leukocyte/endothelium interaction was quantified by in vivo visualization of leukocytes.


RESULTS
Systemic administration of C-peptide showed no influence on wound healing or standard microcirculatory parameters. The leukocyte/ endothelium interaction revealed a significant (p<0.05) increase in the number of adherent leukocytes 15 days after wound creation in C-peptide treated diabetic mice.


CONCLUSIONS
Except for the significantly increased number of leukocytes adherent to venular endothelium in the C-peptide group no alteration was observed in wound healing and microcirculation. Neutrophil recruitment after C-peptide injection is of interest because it may reduce the risk of infection in diabetes mellitus.
Langer
S
S
Clinic for Plastic and Hand Surgery, Burn Center, Ruhr University Bochum, Germany. Stefan.Langer@ruhr-uni-bochum.de
Born
F
F
Breidenbach
A
A
Schneider
A
A
Uhl
E
E
Messmer
K
K
D000818:Animals; D001786:Blood Glucose; D002096:C-Peptide / Q000097:blood / Q000494:pharmacology*; D003911:Dextrans; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D008833:Microcirculation / Q000187:drug effects; D008856:Microscopy, Fluorescence / Q000379:methods; D012867:Skin / Q000098:blood supply* / Q000293:injuries*; D012883:Skin Ulcer / Q000188:drug therapy; D014945:Wound Healing / Q000187:drug effects*
D001786:Blood Glucose; D002096:C-Peptide; D003911:Dextrans; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate