[Screening and sequence analysis of the adult stage-specific gene of Trichinella spiralis].
OBJECTIVE
To obtain the sequence of the adult stage-specific gene of Trichinella spiralis.


METHODS
An adult T. spiralis cDNA library was screened using an adult T. spiralis stage-specific probe. The gene sequence was analyzed by DNAstar software and GenBank database.


RESULTS
A cDNA clone of 1,629 bp was obtained by screening the library with an adult stage-specific probe. The clone contained one open reading frame composed of 1,464 bp encoding 487 amino acids. GenBank searching revealed that no identical cDNA was found. Two zinc fingers located at the positions within amino acids 207-270. Some possible antigen epitopes were predicted within the amino acids 52-64, 108-116, 137-163 and 226-260.


CONCLUSIONS
A novel stage-specific gene of adult T. spiralis encoding the protein containing certain predicted antigen epitopes was obtained.
Yang
J
J
Department of Parasitology, Capital University of Medical Sciences, Beijing 100054.
Zhu
X P
XP
Zhang
X M
XM
Yang
Y P
YP
Zhou
L
L
Gao
X
X
Liu
M Y
MY
Pascal
B
B
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D000939:Epitopes; D015723:Gene Library; D017238:Genes, Helminth; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017160:Trichinella spiralis / Q000235:genetics* / Q000276:immunology
D018076:DNA, Complementary; D000939:Epitopes