title: [Effect of MNNG on ornithine decarboxylase activity in cells from adult Schistosoma japonicum].
abstract: OBJECTIVE
  To observe the change of ornithine decarboxylase(ODC) activity in cells from adult Schistosoma japonicum after the cells were treated with N-Methyl-N-Nitro-N-Nitrosoguanidine (MNNG).
  
  
  METHODS
  The cells were treated with MNNG at a concentration of 3 micrograms/ml for 48 hours after the cells being incubated for one week. The cells were then cultured with RPMI-1640 containing 10% calf serum. ODC activity was detected with spectrophotography.
  
  
  RESULTS
  ODC activity rose significantly in two to three weeks after the cells were treated with MNNG.
  
  
  CONCLUSIONS
  There was ODC activity in cells from adult S. japonicum and MNNG has an effect to reinforce ODC activity in the cells.
authors.affiliation: Department of Parasitology and Microbiology, Hubei Traditional Medical College, Wuhan 430061.
authors.forename: Hong
authors.initials: H
authors.lastname: Fan
authors.forename: Min
authors.initials: M
authors.lastname: Huang
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Man-jun
authors.initials: MJ
authors.lastname: Li
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D012549:Schistosoma japonicum / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology
chemical_list: D008769:Methylnitronitrosoguanidine; D009955:Ornithine Decarboxylase