title: Apoptosis induced by influenza virus-hemagglutinin stimulation may be related to fluctuation of cellular oxidative condition.
abstract: Primary cultivated amnion epitherial cells prepared from amnion tissue of human fetal membrane (Amnion-cells) were stimulated with influenza virus-hemagglutinin (IV-HA), fractionated from a commercialized IV-HA vaccine by DEAE Sephacel column chromatography. From 72-96 h after stimulation, chromosomal DNA fragmentation and the appearance of in situ TUNEL stained-positive cells were revealed. Amnion-cell DNA fragmentation was inhibited in the presence of glycophorin A or C purified from the human erythrocyte membrane fraction, but not inhibited with free N-acetyl-neuraminic acid. RT-PCR and Western blotting analysis showed that anti-oxidative enzymes were altered with incubation period, accompanied by the expression of the cellular oxidative stress-related caspase cascade. Pre-stimulation of Amnion-cells with hemin, a heme oxygenase-1 inducer, significantly attenuated IV-HA induced DNA fragmentation. It is concluded that IV-HA induces apoptosis in Amnion-cells, and that this apoptotic induction may be facilitated by certain sialoglycoproteins on the cell surface, and is related to changes in the intracellular redox condition.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Tokyo University of Pharmacy & Life Science, Horinouchi, Hachioji, Tokyo, Japan. ohyamak@ps.toyaku.ac.jp
authors.forename: Kunio
authors.initials: K
authors.lastname: Ohyama
authors.forename: Manabu
authors.initials: M
authors.lastname: Nishina
authors.forename: Bo
authors.initials: B
authors.lastname: Yuan
authors.forename: Toshio
authors.initials: T
authors.lastname: Bessho
authors.forename: Toshio
authors.initials: T
authors.lastname: Yamakawa
mesh_terms: D000650:Amnion / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D006387:Hemagglutination, Viral; D006801:Humans; D009975:Orthomyxoviridae; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D014765:Viral Vaccines / Q000494:pharmacology
chemical_list: D014765:Viral Vaccines