[Study of the interactions between diorganotin (IV) complexes of 1,3-dimethyl-4-acetyl-5-pyrazolone and mononucleotides and DNA].
OBJECTIVE
The interactions between diorganotin (IV) complexes of 1,3-dimethyl-4-acetyl-5-pyrazolone (HL1) and mono-nucleotides together with DNA near physiological condition were investigated.


METHODS
The mode of action of the diorganotin (IV) complexes with mononucleotides and DNA under different conditions and different times were investigated by high resolution NMR technology and UV spectra.


RESULTS
The interaction of [(L1)2SnEt2] with AMP was shown to result in significant change of chemical shift of H(8), H(2) and 31P of AMP. Hyperchromic effect of DNA could be observed due to the interaction of; [(L1)2SnEt2] with DNA, while interaction of [(L1)2SnMe2] with AMP and DNA could only cause obvious change of chemical shift of 31P and lead to hypochromic effect of DNA.


CONCLUSIONS
The results indicate that [(L1)2SnEt2] can selectively bind to the N1 atom of the base and the phosphate oxygen atom of AMP and may further destroy the helical structure of DNA, while the dimethyltin (IV) compound of 1,3-dimethyl-4-acetyl-5-pyrazolone [(L1)2SnMe2] merely binds to the the phosphate oxygen atom of AMP and causes the contraction of DNA helical structure.
Ning
Mei-ying
MY
School of Pharmaceutical Science, Shanxi Medical University, Third People's Hospital of Taiyuan, Taiyuan 030001, China.
Li
Ting-fang
TF
Li
Qing-shan
QS
D000970:Antineoplastic Agents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D009711:Nucleotides / Q000737:chemistry*; D009947:Organotin Compounds / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry
D000970:Antineoplastic Agents; D009711:Nucleotides; D009947:Organotin Compounds; D011166:Porphyrins; C063166:tin etiopurpurin; D004247:DNA