[Protective effect of Angelica on ECV(304) from injury induced by hyperlipidemic serum in vitro].
The aim of this article was to examine the protective effect of Chinese traditional medicine Angelica on human umbilical vein endothelial cells (HUVECs, ECV(304)) from injury induced by hyperlipidemic serum (HLS) and to study the underlying mechanisms. Microstructures of HUVECs were observed by a scanning electron microscope. Spectrophotometer and immunocytochemical methods were used to detect the content of NO in the suspension and expression of ICAM-1, TGFbeta(1), bFGF on the cell surface, respectively. After being incubated with HLS for 24 hours, HUVECs exhibited pronounced morphological changes, such as disappearance of microvilli on the endothelial cell (EC) surface, rupture of cell membranes, etc. Expression of ICAM-l and bFGF in ECs was significantly increased, while expression of TGFbeta(1) and the release of NO from ECs were significantly decreased. All these effects of HLS on ECs can be reversed by Angelica significantly. The above effects of Angelica may be related to its anti-atherosclerotic action.
Wang
B H
BH
Department of Pathophysiology, Hubei Medical University, Wuhan 43007l.
Ouyang
J P
JP
Liu
Y M
YM
Wei
L
L
Yang
J W
JW
D029971:Angelica sinensis; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001161:Arteriosclerosis / Q000097:blood / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000473:pathology*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006949:Hyperlipidemias / Q000097:blood*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D011817:Rabbits; D014471:Umbilical Veins / Q000473:pathology
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D016222:Fibroblast Growth Factor 2; D018799:Intercellular Adhesion Molecule-1; D009569:Nitric Oxide