title: XY99-5038 promotes long-term survival of cultured retinal neurons.
abstract: We have previously reported that XY99-5038, a preparation from a specific formula of Traditional Chinese Medicine, could effectively inhibit hydrogen peroxide-induced retinal cell death. In the present study, we investigated the possibility of XY99-5038 to prolong neuronal survival in a long-term retinal neuronal culture. Basic fibroblast survival factor (bFGF), a potent neurotrophic factor, was employed as comparable agent. Retinas of 0-2 days old Sprague-Dawley rats were isolated and dissociated. The cells were maintained in tissue culture for up to 9 weeks in a synthetic serum-free media. XY99-5038 (100 ng/ml) or a vehicle was added to culture every 3-4 days, starting at the first week of culturing. The number of cells were counted and compared for each time point and treatment. Cell viability was also determined by MTT assay, whereas apoptotic cell death was evaluated by the TUNEL assay. XY99-5038 treatment significantly reduced cell loss, increased cell viability, and inhibited apoptosis in this long-term retinal neuronal culture. Our data also show that the protective effect of XY99-5038 is more potent than that of bFGF. Our data suggest that XY99-5038 could be beneficial to the prolongation of neuron survival.
authors.affiliation: Institute of Chongqing Traditional Chinese Medicine, Chongqing, Sichuang, P.R. of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yan
authors.forename: Yuhay T
authors.initials: YT
authors.lastname: Fong
authors.forename: Delia
authors.initials: D
authors.lastname: Wolf
authors.forename: Hua
authors.initials: H
authors.lastname: Shen
authors.forename: Marian
authors.initials: M
authors.lastname: Zaharia
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Gerald
authors.initials: G
authors.lastname: Wolf
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Garrick D
authors.initials: GD
authors.lastname: Lee
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002452:Cell Count; D046508:Culture Techniques; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D009433:Neural Inhibition / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D017711:Nonlinear Dynamics; D009930:Organic Chemicals; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000187:drug effects* / Q000473:pathology*; D013995:Time
chemical_list: D018696:Neuroprotective Agents; D009930:Organic Chemicals; C431083:XY99-5038