Increased respiratory burst and increased expression of complement receptor-3 (CD11b/CD18) and of IL-8 receptor-A in neutrophil granulocytes from newborns after vaginal delivery.
To study neutrophil activation in cord blood as a function of the mode of delivery, we performed analysis of the function of neutrophil granulocytes by assessing their ability to produce reactive oxygen products (ROP) as well as neutrophil cell surface expression of CD11b/CD18 and interleukin (IL)-8 receptors quantified with flow cytometry. Plasma levels of granulocyte colony-stimulating factor (G-CSF) were measured using an immunoassay. Neutrophil granulocytes were derived from cord blood of term newborns delivered vaginally (n = 20) and by cesarean section (n = 10), and, for comparison, from adult peripheral blood (n = 15). Blood neutrophil counts and the capacity of neutrophil granulocytes to produce ROP in response to stimulation with Escherichia coli was increased in newborns after vaginal delivery as compared to newborns delivered by cesarean section. The level of expression of the adhesion molecule/complement receptor CD11b/CD18 and the chemokine receptor IL-8 RA was also higher after vaginal delivery. Plasma concentrations of G-CSF in cord blood of newborns were higher than those of adults with no difference detectable between vaginal delivery and cesarean section. The data demonstrate a higher functional responsiveness and a higher expression level of functionally important receptors in neutrophils after vaginal delivery possibly due to activation of neutrophil granulocytes during labor.
Gessler
Peter
P
Intensive Care and Neonatology, University Children's Hospital, Berne, Institute of Immunology, University of Berne, Switzerland. peter.gessler@kispi.unizh.ch
Dahinden
Clemens
C
D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D039481:CD11b Antigen; D018821:CD18 Antigens / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D036861:Delivery, Obstetric; D004926:Escherichia coli / Q000502:physiology; D005260:Female; D005312:Fetal Blood; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood; D006098:Granulocytes / Q000378:metabolism* / Q000382:microbiology; D006801:Humans; D007231:Infant, Newborn; D007958:Leukocyte Count; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism* / Q000382:microbiology; D011247:Pregnancy; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement / Q000378:metabolism; D023062:Receptors, Interleukin-8A / Q000378:metabolism*; D016897:Respiratory Burst
D015703:Antigens, CD; D039481:CD11b Antigen; D018821:CD18 Antigens; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement; D023062:Receptors, Interleukin-8A; D016179:Granulocyte Colony-Stimulating Factor; D009240:N-Formylmethionine Leucyl-Phenylalanine