[Determination of phospholipid in Cibotium barometz by supercritical fluid extraction and RP-HPLC].
A supercritical fluid extraction (SFE) method was used to extract phospholipids in Cibotium barometz. After a homogeneous design experiment, SFE conditions were obtained which were pressure at 31.5 MPa, temperature at 46.2 degrees C and a 0.37 L/kg modifier in supercritical fluid. The sample was extracted for 2 hours. A RP-HPLC method was developed for the determination of phosphatidylcholine. RP-HPLC conditions included a RP-HPLC column packed with Waters Symmetry C18(5 microns), a mobile phase composed of methanol-1% phoshporic acid(90:10) and the detection wavelength at 206 nm. The content of phosphatidylcholine was 0.198%(RSD 4.2%), and the method can be a measure of quality control for Cibotium barometz and its preparations.
Xu
Z
Z
Department of Pharmaceutics of Nanfang Hospital, First Military Medical University, Guangzhou 510515.
Chen
Z
Z
Chen
Z
Z
Ge
F
F
Zhang
K
K
D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D010713:Phosphatidylcholines / Q000032:analysis*; D032494:Polypodium / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D010713:Phosphatidylcholines