A novel strategy of oxygen restriction by some diazotrophic enteric bacteria.
Protection of nitrogenase against oxygen inactivation in diazotrophs involves numerous strategies. Glutathione is known to play an important role in scavenging oxyradicals in many living systems. The involvement of glutathione (reduced) (GSH), glutathione peroxidase (GPX) and glutathione reductase (GR) in the protection of nitrogenase in free living diazotrophs is reported here for the first time. Reduced glutathione content and the activity of glutathione peroxidase and glutathione reductase increased with increase in oxygen concentration under nitrogen fixing conditions but decreased under anaerobic and nitrogenase repressed conditions. This correlation is used to postulate a protecting role for GSH-GPX-GR system against oxygen inactivation of nitrogenase.
Kannan
V
V
Centre for Advanced Study in Botany, University of Madras, Guindy Campus, Chennai 600 025, India. kannanavo@yahoo.co.in
Raju
P N
PN
D004755:Enterobacteriaceae / Q000187:drug effects* / Q000378:metabolism; D005609:Free Radicals / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism*; D009586:Nitrogen Fixation; D009591:Nitrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000494:pharmacology*
D005609:Free Radicals; D005979:Glutathione Peroxidase; D009591:Nitrogenase; D005980:Glutathione Reductase; D005978:Glutathione; D010100:Oxygen