[Effect of nitroquine on the membrane phospholipid of intraerythrocytic Plasmodium yoelii in vitro].
OBJECTIVE
To study the mechanism of antimalarial action of nitroquine.


METHODS
Intraerythrocytic P. yoelii was cultured by the method of Trager and Jensen. The amount of [3H]-ethanolamine incorporation was measured as an index of the phospholipid synthesis. DPH was used as a probe to measure the plasmodial fluorescent polarization.


RESULTS
The incorporation of [3H]-ethanolamine into the P. yoelii infected erthrocytes was markedly inhibited by nitroquine. The plasmodial membrane polarization and viscosity were significantly increased by nitroquine.


CONCLUSIONS
Nitroquine could inhibit the phospholipid synthesis and decrease the membrane fluidity of P. yoelii.
Deng
S
S
Department of Pharmacology, Third Military Medical University, Chongqing 400038.
Hu
Y
Y
D000818:Animals; D000852:Anopheles / Q000187:drug effects / Q000378:metabolism; D000962:Antimalarials / Q000494:pharmacology*; D022082:Cellular Structures / Q000187:drug effects; D004983:Ethanolamines / Q000378:metabolism; D008288:Malaria / Q000235:genetics / Q000378:metabolism; D008566:Membranes / Q000187:drug effects* / Q000378:metabolism; D021261:Parasitic Sensitivity Tests; D010743:Phospholipids / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000187:drug effects / Q000378:metabolism; D010967:Plasmodium yoelii / Q000187:drug effects* / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics; D011739:Pyrimethamine / Q000378:metabolism; D011799:Quinazolines / Q000494:pharmacology*
D000962:Antimalarials; D004983:Ethanolamines; D010743:Phospholipids; D015800:Protozoan Proteins; D011799:Quinazolines; C021126:nitroquine; D011739:Pyrimethamine