[Hair cell apoptosis and hearing loss of perilymphatic fistula].
OBJECTIVE
To investigate the effect of hair cell apoptosis on hearing loss of perilymphatic fistula (PLF) in guinea pig.


METHODS
Twenty-five guinea pigs with light microscope and TdT mediated biotin dUTP nick-end labelling (TUNEL) techniques. The ECochG and ABR were measured and the data analyzed with statistics.


RESULTS
1. The apoptosis of hair cell was not revealed in 0-hour-group and 2-hour-group of PLF. Following the time of PLF was longer, the apoptosis of hair cell was more increased. The apoptosis of hair cell was demonsted in 1-day-group (1 case, 17%), 2-day-group (4 case, 67%) and 7-day-group (4 case, 80%); 2. The amplication of AP was reduced after operation induced PLF. The CAP of experimental ear was significantly higher than that of control ear (P < 0.01) in 1-day-group, 2-day-group and 7-day-group.


CONCLUSIONS
1. Apoptosis of hair cell was appeared in PLF; 2. The apoptosis of hair cell may be one of morphological evidence in hearing loss of PLF.
Chen
J
J
Department of Otorhinolaryngology, Second Clinical Medical College Hunan Medical University, Changsha 410011.
Lu
Y
Y
Ren
J
J
Chen
Z
Z
Xie
D
D
D000818:Animals; D017209:Apoptosis; D005260:Female; D005402:Fistula / Q000473:pathology*; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000473:pathology* / Q000503:physiopathology; D006319:Hearing Loss, Sensorineural / Q000473:pathology*; D008297:Male; D010498:Perilymph