title: Effects of amniotic membrane transplantation and mitomycin C on wound healing in experimental glaucoma surgery.
abstract: OBJECTIVE
  To evaluate the effects of mitomycin C and amniotic membrane transplantation on the modulation of wound healing after glaucoma filtration surgery.
  
  
  METHODS
  Trabeculectomy was performed in 3 groups of 10 rabbit eyes each. Sponges soaked in mitomycin C were applied to the first group, and amniotic membranes were transplanted around the scleral flap in the second group; the third group was the control. The arithmetic means of fibroblast and macrophage numbers per square centimetre were calculated.
  
  
  RESULTS
  The mean numbers of fibroblasts and macrophages were decreased significantly in the mitomycin C group, compared with the controls (p < 0.0001). Cell counts in the amniotic membrane group were also lower than the ones in the control group for fibroblasts and macrophages (p < 0.0001, p < 0.01, respectively). The difference between the two treatment groups was statistically significant for fibroblasts (p < 0.0001), but not significant for macrophages (p > 0.05).
  
  
  CONCLUSIONS
  In spite of the fact that amniotic membrane transplantation is less effective than mitomycin C for depressing wound healing after trabeculectomy, it might be a safer alternative in selected cases when considering severe adverse effects of antimetabolites.
authors.affiliation: Department of Ophthalmology, Firat University Medical School, Elazig, Turkey. tameralperumay@yahoo.com
authors.forename: Tamer
authors.initials: T
authors.lastname: Demir
authors.forename: Burak
authors.initials: B
authors.lastname: Turgut
authors.forename: Nuray
authors.initials: N
authors.lastname: Akyol
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Ozercan
authors.forename: Fatih
authors.initials: F
authors.lastname: Ulaş
authors.forename: Ulkü
authors.initials: U
authors.lastname: Celiker
mesh_terms: D000287:Administration, Topical; D000650:Amnion / Q000473:pathology / Q000637:transplantation*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology* / Q000627:therapeutic use; D005347:Fibroblasts / Q000473:pathology; D005901:Glaucoma / Q000188:drug therapy / Q000601:surgery*; D007429:Intraocular Pressure / Q000187:drug effects; D007430:Intraoperative Care; D008264:Macrophages / Q000473:pathology; D016685:Mitomycin / Q000494:pharmacology* / Q000627:therapeutic use; D013508:Ophthalmologic Surgical Procedures; D011817:Rabbits; D014130:Trabeculectomy; D014182:Transplantation, Autologous; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D000903:Antibiotics, Antineoplastic; D016685:Mitomycin