[Study on the structural property of polysacchrides 139A--a kind of IL-1R antagonists from microorganism].
OBJECTIVE
To study the structural property of the bioactive component from the fermentation broth of Streptomyces 139 which can inhibit IL-1R.


METHODS
Polysaccharide 139A is extracted and purified by means of macroreticular resin Diaion HP-20, cation exchange and DEAE Sephadex A-25 chromatography. The sugar compositions were analyzed with GC, the linkage positions of 139A were determined by methylation and GC-MS.


RESULTS
The bioactive component which can inhibit IL-1R is a kind of polysaccharides.


CONCLUSIONS
The components of 139A repeat units are conjectured.
Chen
J
J
Institute of Medical Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Wu
J B
JB
Liu
Y M
YM
Xu
G Y
GY
D008401:Gas Chromatography-Mass Spectrometry; D008745:Methylation; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors*; D013302:Streptomyces / Q000737:chemistry*
D011135:Polysaccharides, Bacterial; D017472:Receptors, Interleukin-1