Characterization of the genomic structure and expression of the mouse Apex2 gene.
We isolated a mouse cDNA encoding APEX2 protein and demonstrated that APEX2 binds to PCNA. The level of Apex2 mRNA was high in the thymus, bone marrow, spleen, and kidney in adult mice. Apex2 consists of six exons and is flanked on the 3' end by Alas2 on X chromosome 63.0. Furthermore, Apex2 is flanked on the 5' end by a novel gene with a 106-bp intergenic sequence. We disrupted Apex2 in embryonic stem cells derived from a male mouse, and a 55-kDa APEX2 protein was detected in the nuclei of Apex2(+) but not Apex2-disrupted cells. Immunoelectron microscopy revealed that APEX2 is also localized in the mitochondria of Apex2(+) cells. In serum-stimulated BALB/c 3T3 cells, the level of Apex2 mRNA was transiently increased and the level of APEX2 reached a maximum in the late S phase, thus indicating that APEX2 may participate in postreplicative base excision repair.
Ide
Yasuhito
Y
Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Fukuoka 812-8582, Japan.
Tsuchimoto
Daisuke
D
Tominaga
Yohei
Y
Iwamoto
Yukihide
Y
Nakabeppu
Yusaku
Y
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D004260:DNA Repair / Q000235:genetics* / Q000502:physiology; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism; D018390:Gene Targeting; D051379:Mice; D008969:Molecular Sequence Data; D064251:Multifunctional Enzymes
D064251:Multifunctional Enzymes; C476886:Apex2 protein, mouse; D004720:Endonucleases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase