Bacteriocin detection from whole bacteria by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
Class I bacteriocins (lantibiotics) and class II bacteriocins are antimicrobial peptides secreted by gram-positive bacteria. Using two lantibiotics, lacticin 481 and nisin, and the class II bacteriocin coagulin, we showed that bacteriocins can be detected without any purification from whole producer bacteria grown on plates by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS). When we compared the results of MALDI-TOF-MS performed with samples of whole cells and with samples of crude supernatants of liquid cultures, the former samples led to more efficient bacteriocin detection and required less handling. Nisin and lacticin 481 were both detected from a mixture of their producer strains, but such a mixture can yield additional signals. We used this method to determine the masses of two lacticin 481 variants, which confirmed at the peptide level the effect of mutations in the corresponding structural gene.
Hindré
Thomas
T
Laboratoire de Biologie et Chimie Moléculaires, EA 2594, Université de Bretagne Sud, 56321 Lorient, France.
Didelot
Sandrine
S
Le Pennec
Jean-Paul
JP
Haras
Dominique
D
Dufour
Alain
A
Vallée-Réhel
Karine
K
D001407:Bacillus / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001430:Bacteriocins / Q000378:metabolism*; D003470:Culture Media; D013294:Lactococcus lactis / Q000254:growth & development / Q000378:metabolism*; D009561:Nisin / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013925:Thromboplastin / Q000378:metabolism
D001426:Bacterial Proteins; D001430:Bacteriocins; D003470:Culture Media; C082508:lacticin 481; D009561:Nisin; D013925:Thromboplastin