title: [Humoral immune response in mice to hybrid nucleic acid vaccines containing Plasmodium falciparum merozoite surface protein 1 block 17-based gene].
abstract: OBJECTIVE
  To analyse the humoral immune response in mice to nucleic acid vaccines (VR1012/HG-MSP1-17 for intracellular expression or VR1012/TPA/HG-MSP-17 for secretion) containing Plasmodium falciparum merozoite surface protein 1 (MSP1) 17 block gene and gene fragment of several T cell epitopes from MSA1, MSA2, RESA, IL-1 and TT.
  
  
  METHODS
  BALB/c or C57BL/6 mice received three times intramuscular immunization with 200 micrograms/100 microliters or 100 micrograms/100 microliters of VR1012/HG-MSP1-17 or VR1012/TPA/HG-MSP1-17 per mouse each time. Anti-HG or anti-MSP1-17 antibodies were monitored by indirect ELISA.
  
  
  RESULTS
  BALB/c and C57BL/6 mice immunized with 100 micrograms/100 microliters of VR1012/HG-MSP1-17 per mouse raised significantly anti-HG and anti-MSP1-17 antibodies, but the levels of antibodies were not high. BALB/c mice immunized with 200 micrograms/100 microliters of VR1012/HG-MSP1-17 per mouse raised higher anti-HG antibodies but not anti-MSP1-17 antibodies. BALB/c mice immunized with 200 micrograms/100 microliters of VR1012/TPA/HG-MSP1-17 per mouse raised low level of anti-HG antibodies only.
  
  
  CONCLUSIONS
  VR1012/HG-MSP1-17 is more immunogenic than VR1012/TPA/HG-MSP1-17.
authors.affiliation: Department of Parasitology, Fourth Military Medical University, Xi'an 710032.
authors.forename: J
authors.initials: J
authors.lastname: Miao
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: C
authors.initials: C
authors.lastname: Xue
authors.forename: R
authors.initials: R
authors.lastname: Zhen
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: E
authors.initials: E
authors.lastname: Qin
authors.forename: Q
authors.initials: Q
authors.lastname: Yu
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D000953:Antigens, Protozoan / Q000276:immunology / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D017780:Malaria Vaccines / Q000276:immunology / Q000494:pharmacology*; D020066:Merozoite Surface Protein 1 / Q000276:immunology / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010963:Plasmodium falciparum / Q000737:chemistry / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology / Q000494:pharmacology*; D019444:Vaccines, DNA / Q000276:immunology / Q000494:pharmacology
chemical_list: D000953:Antigens, Protozoan; D017780:Malaria Vaccines; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins; D019444:Vaccines, DNA