PCR-based identification of Bacillus thuringiensis pesticidal crystal genes.
The polymerase chain reaction (PCR) is a molecular tool widely used to characterize the insecticidal bacterium Bacillus thuringiensis. This technique can be used to amplify specific DNA fragments and thus to determine the presence or absence of a target gene. The identification of B. thuringiensis toxin genes by PCR can partially predict the insecticidal activity of a given strain. PCR has proven to be a rapid and reliable method and it has largely substituted bioassays in preliminary classification of B. thuringiensis collections. In this work, we compare the largest B. thuringiensis PCR-based screenings, and we review the natural occurrence of cry genes among native strains. We also discuss the use of PCR for the identification of novel cry genes, as well as the potential of novel technologies for the characterization of B. thuringiensis strains.
Porcar
Manuel
M
Laboratoire des Bactéries et Champignons Entomopathogènes, Institut Pasteur, 25 rue du Dr. Roux, 75724, Paris Cedex 15, France.
Juárez-Pérez
Victor
V
D001413:Bacillus thuringiensis / Q000235:genetics* / Q000302:isolation & purification; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D001483:Base Sequence; D004731:Endotoxins / Q000235:genetics*; D005787:Gene Frequency; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D007306:Insecticides; D010572:Pest Control, Biological / Q000639:trends; D016133:Polymerase Chain Reaction / Q000379:methods*; D017385:Sequence Homology
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis