Lack of association of genetic polymorphisms in the interleukin-1beta, interleukin-1 receptor antagonist, interleukin-4 and interleukin-10 genes with mitral valve prolapse in Taiwan Chinese.
OBJECTIVE
Inflammation and genetics may play a role in the pathogenesis of mitral valve prolapse (MVP). The study aim was to test whether interleukin-1beta (IL-1beta), IL-1 receptor antagonist (IL-1Ra), IL-4 or IL-10 gene polymorphisms could be used as markers of susceptibility or severity in MVP among the Chinese population in Taiwan.


METHODS
A group of 100 patients with MVP diagnosed echocardiographically, and 103 age- and sex-matched normal control subjects was studied. IL-1beta promoter, IL-1beta exon 5, IL-1Ra, IL-4 promoter, IL-4 intron 3 and IL-10 gene polymorphisms were identified by polymerase chain reaction-based restriction analysis.


RESULTS
There was no significant difference in the distribution of genotypes and allelic frequencies between MVP cases and controls for IL-1beta promoter, IL-1beta exon 5, IL-1Ra, IL-4 promoter, IL-4 intron 3 and IL-10 gene polymorphisms. Further categorization of MVP patients into mild and severe subgroups also revealed no statistical difference in these gene polymorphisms when compared with controls.


CONCLUSIONS
These findings suggest that the IL-1beta, IL-1Ra, IL-4 or IL-10 gene polymorphisms are not suitable genetic markers of MVP in Taiwan Chinese.
Chou
Hsiang-Tai
HT
Division of Cardiology, Department of Medicine, China Medical College Hospital, Taichung, Taiwan.
Shi
Yi-Ru
YR
Hsu
Yuan
Y
Tsai
Fuu-Jen
FJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D002681:China / Q000208:ethnology; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D015847:Interleukin-4 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008945:Mitral Valve Prolapse / Q000235:genetics*; D011110:Polymorphism, Genetic; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors*; D013624:Taiwan
D005819:Genetic Markers; D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D016753:Interleukin-10; D015847:Interleukin-4