Ischemic rat brain extracts induce human marrow stromal cell growth factor production.
Intravenous administration of human bone marrow stromal cells (hMSCs) after middle cerebral artery occlusion (MCAo) in rats provides functional benefit. We tested the hypothesis that these functional benefits are derived in part from hMSC production of growth and trophic factors. Quantitative sandwich enzyme-linked immunosorbent assay (ELISA) of hMSCs cultured with normal and MCAo brain extracts were performed. hMSCs cultured in supernatant derived from ischemic brain extracts increased production of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF). These neurotrophins and angiogenic growth factors increased in a post-ischemia time-dependent manner. The hMSC capacity to increase expression of growth and trophic factors may be the key to the benefit provided by transplanted hMSCs in the ischemic brain.
Chen
Xiaoguang
X
Department of Neurology, Division of Hematology/Medical Oncology, Henry Ford Health Sciences Center, Detroit, USA.
Li
Yi
Y
Wang
Lei
L
Katakowski
Mark
M
Zhang
Lijie
L
Chen
Jieli
J
Xu
Yongxian
Y
Gautam
Subhash C
SC
Chopp
Michael
M
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism; D017690:Cell Transplantation; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006133:Growth Substances / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D051381:Rats; D017154:Stromal Cells / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors; D014020:Tissue Extracts / Q000494:pharmacology*
D006133:Growth Substances; D014020:Tissue Extracts