title: [Generation of Dendritic Cells from Human Cord Blood CD34(+) Cells and Their Activation of T Cell-Dependent Antitumor Immune Response In Vitro]
abstract: To induce the growth and differentiation of dendritic cells (DCs) from human cord blood, CD34(+) cells isolated from human cord blood by mini-MACS were cultured in a liquid culture system with rhSCF, rhGM-CSF, rhTNF-alpha and rhFL for 10 days. Then the induced cells were characterized by DC's morphological and phenotypic properties. In addition, they stimulated the proliferation of allogeneic T cells and possessed an efficient capacity for initiating T cell-dependent antitumor immune responses in vitro. It is concluded that mature DCs could be obtained from human cord blood CD34(+) cells.
authors.affiliation: Department of Hematology and Oncology, The First Clinical Hospital, Jilin University, Changchun 130021, China; Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Guan-Jun
authors.initials: GJ
authors.lastname: Wang
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Xie
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Kai
authors.initials: K
authors.lastname: Feng
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.forename: Xue-Tao
authors.initials: XT
authors.lastname: Pei