Cloning and molecular characterization of the salt-regulated jojoba ScRab cDNA encoding a small GTP-binding protein.
Salt stress results in a massive change in gene expression. An 837 bp cDNA designated ScRab was cloned from shoot cultures of the salt tolerant jojoba (Simmondsia chinesis). The cloned cDNA encodes a full length 200 amino acid long polypeptide that bears high homology to the Rab subfamily of small GTP binding proteins, particularly, the Rab5 subfamily. ScRab expression is reduced in shoots grown in the presence of salt compared to shoots from non-stressed cultures. His6-tagged ScRAB protein was expressed in E. coli, and purified to homogeneity. The purified protein bound radiolabelled GTP. The unlabelled guanine nucleotides GTP, GTP gamma S and GDP but not ATP, CTP or UTP competed with GTP binding.
Mizrahi-Aviv
Ela
E
Department of Life Sciences, Doris and Bertie Center for Bioenergetics in Life Sciences, Ben-Gurion University, Beer-Sheva 84105, Israel.
Mills
David
D
Benzioni
Aliza
A
Bar-Zvi
Dudy
D
D000595:Amino Acid Sequence; D001483:Base Sequence; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D006160:Guanosine Triphosphate / Q000378:metabolism; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins