title: Time dependent appearance of selected apoptotic markers and usefulness of their detection in vitro.
abstract: Many experiments have demonstrated that some cell lines are resistant to chemically induced apoptosis in vitro, and that apoptosis itself is far from being a homogenous phenomenon. Here we show that 10 microg/ml etoposide elicited only minor changes in Bowes human melanoma cells (temporary decrease in cell viability and proliferation, transient phospatidylserine externalization and caspase-3 activation), which weren't clearly capable to start apoptotic pathway in the entire treated population. On the other hand, potassium chromate at concentration of 150 microg/ml executed cell death bearing some features of apoptosis (cell blebbing, caspase-3 activation and cytoskeletal changes) but lacking or showing weakly others (DNA fragmentation and phospatidylserine externalization). Our results suggest that in detecting apoptosis several fault-proof detection systems are to be used to avoid misleading results and conclusions in each experimental setting.
authors.affiliation: Charles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biology and Genetics, Czech Republic. rudolf@lfhk.cuni.cz
authors.forename: Emil
authors.initials: E
authors.lastname: Rudolf
authors.forename: Miroslav
authors.initials: M
authors.lastname: Cervinka
mesh_terms: D000199:Actins / Q000378:metabolism; D017304:Annexin A5 / Q000032:analysis; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000032:analysis*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002455:Cell Division; D002840:Chromates / Q000494:pharmacology; D053938:DNA Fragmentation / Q000187:drug effects; D004273:DNA, Neoplasm / Q000032:analysis / Q000187:drug effects; D005047:Etoposide / Q000494:pharmacology; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006801:Humans; D008546:Melanoma, Experimental / Q000737:chemistry / Q000473:pathology; D018715:Microscopy, Video; D010718:Phosphatidylserines / Q000032:analysis; D017680:Potassium Compounds / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000473:pathology
chemical_list: D000199:Actins; D017304:Annexin A5; D015415:Biomarkers; D002840:Chromates; D004273:DNA, Neoplasm; D005456:Fluorescent Dyes; D010718:Phosphatidylserines; D017680:Potassium Compounds; C027373:potassium chromate(VI); D005047:Etoposide; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; D016650:Fluorescein-5-isothiocyanate