TES is a novel focal adhesion protein with a role in cell spreading.
Previously, we identified TES as a novel candidate tumour suppressor gene that mapped to human chromosome 7q31.1. In this report we demonstrate that the TES protein is localised at focal adhesions, actin stress fibres and areas of cell-cell contact. TES has three C-terminal LIM domains that appear to be important for focal adhesion targeting. Additionally, the N-terminal region is important for targeting TES to actin stress fibres. Yeast two-hybrid and biochemical analyses yielded interactions with several focal adhesion and/or cytoskeletal proteins including mena, zyxin and talin. The fact that TES localises to regions of cell adhesion suggests that it functions in events related to cell motility and adhesion. In support of this, we demonstrate that fibroblasts stably overexpressing TES have an increased ability to spread on fibronectin.
Coutts
Amanda S
AS
Cancer Research UK Laboratories, Beatson Institute for Cancer Research, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.
MacKenzie
Elaine
E
Griffith
Elen
E
Black
Donald M
DM
D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D003598:Cytoskeletal Proteins; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D022001:Focal Adhesions / Q000378:metabolism*; D016147:Genes, Tumor Suppressor; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D060588:LIM Domain Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008667:Metalloproteins / Q000235:genetics / Q000378:metabolism; D008840:Microfilament Proteins; D018613:Microscopy, Confocal; D011485:Protein Binding; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016608:Talin / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D025521:Tumor Suppressor Proteins; D020798:Two-Hybrid System Techniques; D060589:Zyxin
D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; C102176:Enah protein, mouse; D006023:Glycoproteins; D018398:Homeodomain Proteins; D060588:LIM Domain Proteins; D008164:Luminescent Proteins; D008667:Metalloproteins; D008840:Microfilament Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; C429688:TES protein, human; D016608:Talin; D025521:Tumor Suppressor Proteins; C092842:ZYX protein, human; C498285:Zyx protein, mouse; D060589:Zyxin; D049452:Green Fluorescent Proteins; D005982:Glutathione Transferase