Optimization of sorghum transformation parameters using genes for green fluorescent protein and beta-glucuronidase as visual markers.
Early and reliable detection of plant transformation events is essential for establishing efficient transformation protocols. We have compared the effectiveness of using the gene encoding a green fluorescent protein (GFP) and a beta-glucuronidase (gus) as reporter genes for early detection of transgene expression in explants subjected to biolistic bombardment and Agrobacterium-mediated transformation. The results indicate that gfp gene is superior to gus gene in following transgene expression in transiently transformed materials in both methods of transformation. Using GFP as the screenable marker, we have optimized sorghum transformation with respect to the conditions for transformation, type of explants, promoters, and inbreds. These optimized conditions have been used to obtain stably transformed explants for subsequent regeneration.
Jeoung
J M
JM
Department of Agronomy, Kansas State University, Manhattan, KS, USA.
Krishnaveni
S
S
Muthukrishnan
S
S
Trick
H N
HN
Liang
G H
GH
D019470:Biolistics; D002523:Edible Grain / Q000235:genetics*; D005819:Genetic Markers; D005966:Glucuronidase / Q000235:genetics*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics; D014170:Transformation, Genetic
D005819:Genetic Markers; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D005966:Glucuronidase