Upregulation of purinergic P2Y receptor-mediated calcium responses in glial cells during experimental detachment of the rabbit retina.
To investigate injury-induced alterations of purinergic P2Y receptor-mediated calcium responses in glial (Müller) cells of the rabbit retina, neural retinae were experimentally detached from the pigment epithelium. The ATP-evoked calcium responses were recorded in the endfeet of glial cells at the vitread surface of retinal wholemounts. In control retinae, approximately 7% of the glial cells investigated showed ATP-evoked calcium responses. Within 24 h of detachment, significantly more retinal glial cells (42%) showed calcium responses, and glial ATP responsiveness increased further in retinae which were detached for 48 (44%) or for 72 h (64%). The results indicate that in the detached retina, glial cells upregulate their responsiveness to extracellular ATP within 24 h of injury. Thus, P2Y receptor-mediated signalling may be involved in the early steps of glial response to retinal injury.
Uckermann
Ortrud
O
Department of Neurophysiology, Paul Flechsig Institute of Brain Research, University of Leipzig, D-04109, Leipzig, Germany.
Uhlmann
Susann
S
Weick
Michael
M
Pannicke
Thomas
T
Francke
Mike
M
Reichenbach
Andreas
A
Wiedemann
Peter
P
Bringmann
Andreas
A
D000818:Animals; D020013:Calcium Signaling; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D018613:Microscopy, Confocal; D009457:Neuroglia / Q000378:metabolism* / Q000473:pathology; D011817:Rabbits; D018048:Receptors, Purinergic P2 / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*; D012163:Retinal Detachment / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation
D018048:Receptors, Purinergic P2