Abnormal expression of apoptosis-related genes in haematological malignancies: overexpression of MYC is poor prognostic sign in mantle cell lymphoma.
The expression of apoptosis-related genes BCL2, BAX, BCL2L1, BCL2A1, MCL1, DAPK1 and MYC was studied by quantitative real-time polymerase chain reaction on total RNA samples from patients with acute lymphoblastic leukaemia (ALL, n = 16), acute myeloid leukaemia (AML, n = 27), chronic myeloid leukaemia (CML, n = 12), mantle cell lymphoma (MCL, n = 19) and chronic lymphoid leukaemia (CLL, n = 32). BCL2, BAX, BCL2A1, MCL1, DAPK1 and MYC were overexpressed in all patient groups. BCL2L1 was underexpressed in CLL and CML, but not in AML, ALL and MCL. MCL1 levels were significantly higher in CD13 and CD33-positive ALL, and in CD56-positive AML samples. BCL2, BCL2L1, BCL2A1 and MCL1 were overexpressed and DAPK1 was underexpressed in CLL samples with a 11q23 deletion. MYC overexpression was significantly associated with shorter overall survival in MCL (P < 0.01). AML patients with a normal karyotype showed a higher frequency of BCL2A1 overexpression (P < 0.001) than those with an abnormal karyotype.
Nagy
Bálint
B
Department of Pathology and Medical Genetics, Haartman Institute, University of Helsinki, Helsinki, Finland.
Lundán
Tuija
T
Larramendy
Marcelo L
ML
Aalto
Yan
Y
Zhu
Ying
Y
Niini
Tarja
T
Edgren
Henrik
H
Ferrer
Anna
A
Vilpo
Juhani
J
Elonen
Erkki
E
Vettenranta
Kim
K
Franssila
Kaarle
K
Knuutila
Sakari
S
D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D017353:Gene Deletion; D015870:Gene Expression; D016259:Genes, myc; D006801:Humans; D016130:Immunophenotyping; D007938:Leukemia / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D020522:Lymphoma, Mantle-Cell / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate
D014408:Biomarkers, Tumor