Function of beta-adrenergic receptors on mononuclear cells in female patients with fibromyalgia.
OBJECTIVE
To investigate the beta-adrenergic receptors (beta AR) in patients with chronic fibromyalgia syndrome (FM). These receptors are present on circulating mononuclear cells, and activation of G-protein coupled receptors like beta AR leads to an increase in the intracellular level of cyclic aminosine monophosphate (cAMP). Therefore, cAMP levels can be used to indirectly assess the functional status of the receptor.


METHODS
Eight female patients with FM and 9 matched healthy female controls participated in this study. Blood samples were drawn from subjects' anterocubital vein in the morning. Mononuclear cells were isolated from the whole blood according to Boyüm's method. Basal and stimulated intracellular cAMP levels were determined by enzyme immunoassay. Aliquots of 10(6) cells were incubated with or without stimulation of beta-agonist isoproterenol for 5 min. Two different concentrations of isoproterenol (10(-3) M and 10(-5) M) were utilized.


RESULTS
The basal cAMP levels in patients with FM (3.02 +/- 0.44 pmol/10(6) cells) were slightly more elevated (but not statistically different; p = 0.124, Mann-Whitney U test) than that of the control group (2.26 +/- 0.39 pmol/10(6) cells). Proterenol 10(-3) M stimulation significantly increased intracellular cAMP from the basal levels in both groups (FM group, p = 0.008; control group, p = 0.011). However, isoproterenol 10(-5) M did not increase mean intracellular cAMP levels in the FM group (p = 0.74), while a significant increase was observed in the control group (p = 0.012).


CONCLUSIONS
These preliminary results suggest that diminished beta AR function is associated with the chronic FM state.
Maekawa
Kenji
K
Division of Oral Biology and Medicine, UCLA School of Dentistry, Los Angeles, California 90095, USA.
Twe
Cheryl
C
Lotaif
Ana
A
Chiappelli
Francesco
F
Clark
Glenn T
GT
D000328:Adult; D002908:Chronic Disease; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D005356:Fibromyalgia / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008875:Middle Aged; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*
D011943:Receptors, Adrenergic, beta; D000242:Cyclic AMP