title: Phytosterols affect endocrinology and metabolism of the field vole (Microtus agrestis).
abstract: Phytosterols or plant sterols (PS) enter the ecosystem via pulp mill effluents. They are also consumed by the general population of developed countries in natural remedies and margarines to lower elevated serum cholesterol levels. This study screened the endocrine and enzymatic parameters of the field vole (Microtus agrestis) for the effects of subchronic PS exposure at three doses (0, 5, or 50 mg of PS kg(-1) day(-1)). PS at 5 or 50 mg kg(-1) day(-1) decreased the relative liver weight of the voles. The kidney glycogen phosphorylase activity decreased at 5 or 50 mg kg(-1) day(-1), but the liver glycogen phosphorylase activity increased at 5 mg kg(-1) day(-1). The plasma estradiol and testosterone concentrations of males were higher due to PS supplement at 5 mg kg(-1) day(-1). This can be due to increased sex steroid synthesis from PS precursors. Biotransformation enzyme activities were not affected. PS caused multiple, previously unreported effects that were more pronounced at a low dose. As 5 mg PS kg(-1) day(-1) is the recommended dose for various health products, a thorough risk assessment of the effects and interactions of PS is warranted.
authors.affiliation: Department of Biology, University of Joensuu, FIN-80101 Joensuu, Finland. pniemine@cc.joensuu.fi
authors.forename: Petteri
authors.initials: P
authors.lastname: Nieminen
authors.forename: Anne-Mari
authors.initials: AM
authors.lastname: Mustonen
authors.forename: Pirjo
authors.initials: P
authors.lastname: Lindström-Seppä
authors.forename: Virve
authors.initials: V
authors.lastname: Kärkkäinen
authors.forename: Helena
authors.initials: H
authors.lastname: Mussalo-Rauhamaa
authors.forename: Jussi V K
authors.initials: JV
authors.lastname: Kukkonen
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000378:metabolism*; D001835:Body Weight; D050260:Carbohydrate Metabolism; D004435:Eating; D005260:Female; D012739:Gonadal Steroid Hormones / Q000097:blood; D006801:Humans; D007668:Kidney / Q000201:enzymology*; D050356:Lipid Metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D009929:Organ Size; D010840:Phytosterols / Q000378:metabolism*; D011863:Radioimmunoassay; D011897:Random Allocation
chemical_list: D012739:Gonadal Steroid Hormones; D010840:Phytosterols