Diagnosis of visceral leishmaniasis: comparative potential of amastigote antigen, recombinant antigen and PCR.
Development of simple, economical and non-invasive tests for the early diagnosis of visceral leishmaniasis (VL) or kala-azar (KA) remains a challenge, and serological studies based on antigen prepared from the amastigote stage of Leishmania donovani, the stage that causes infection, are lacking. In the present study, circulating antibodies to total antigen isolated from the promastigote and amastigote stages of the parasite, as well as to recombinant K39 (rK39) antigen, are measured by enzyme-linked immunosorbent assay (ELISA) and the results compared with a polymerase chain reaction (PCR) test for KA diagnosis. In 116 samples of KA examined, the amastigote antigen gave significantly higher mean absorbance values in ELISA than did the promastigote antigen. The sensitivity for KA detection was significantly higher using the amastigote antigen (94%) than the promastigote antigen (90.5%). Analysis in 91 controls showed that specificity was higher with amastigote antigen (92.3%) than with promastigote antigen (86.8-89.0%). Reliability of ELISA diagnosis with amastigote antigen was only marginally lower than that with rK39 ELISA or with the PCR test. Easy availability and low cost of indigenous amastigote antigen, together with the simplicity of ELISA compared with PCR, make ELISA based on amastigote antigen a promising choice for the diagnosis of KA.
Sreenivas
G
G
Institute of Pathology, Safdarjung Hospital Campus, New Delhi-110 029, India.
Ansari
N A
NA
Singh
R
R
Subba Raju
B V
BV
Bhatheja
R
R
Negi
N S
NS
Salotra
R
R
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007893:Leishmania donovani / Q000276:immunology; D007898:Leishmaniasis, Visceral / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods; D011994:Recombinant Proteins / Q000276:immunology
D000953:Antigens, Protozoan; D011994:Recombinant Proteins