title: Influence of NaOCl deproteinization on shear bond strength in function of dentin depth.
abstract: OBJECTIVE
  To determine the influence of NaOCl application on shear bond strengths (SBS) to superficial and deep dentin, using Prime & Bond 2.1.
  
  
  METHODS
  Superficial and deep dentin was exposed in 40 extracted third molars by sectioning the occlusal surface immediately under the enamel-dentin junction or close to the pulp chamber. After polishing the dentin disks (600-grit SiC), they were assigned to two groups: (1) 36% orthophosphoric acid for 15 seconds, or (2): 36% orthophosphoric acid for 15 seconds followed by 5% sodium hypochlorite (NaOCl) for 2 minutes. The dentin adhesive was applied as per manufacturer's instructions, followed by TPH resin-based composite. The specimens were stored in water for 24 hours at 37 degrees C and thermocycled x500 and SBS were determined. Data were statistically analyzed with ANOVA and Student's t-test.
  
  
  RESULTS
  For acid-etched specimens, superficial dentin resulted in statistically higher mean SBS than deep dentin. After NaOCl application, deep and superficial dentin resulted in statistically similar mean SBS. Collagen removal did not affect mean SBS on superficial dentin, while SBS values on deep dentin were increased.
authors.affiliation: Department of Dental Materials, School of Dentistry, University of Granada, Spain. toledano@platon.ugr.es
authors.forename: Manuel
authors.initials: M
authors.lastname: Toledano
authors.forename: Jorge
authors.initials: J
authors.lastname: Perdigão
authors.forename: Estrella
authors.initials: E
authors.lastname: Osorio
authors.forename: Raquel
authors.initials: R
authors.lastname: Osorio
mesh_terms: D000096:Acetone; D000704:Analysis of Variance; D003094:Collagen / Q000187:drug effects; D003188:Composite Resins; D001840:Dental Bonding; D003799:Dental Stress Analysis; D003804:Dentin / Q000737:chemistry / Q000187:drug effects*; D003806:Dentin Permeability / Q000187:drug effects*; D017220:Dentin-Bonding Agents; D006801:Humans; D008422:Materials Testing; D008963:Molar; D011109:Polymethacrylic Acids; D033081:Shear Strength; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D013718:Tensile Strength
chemical_list: D003188:Composite Resins; D017220:Dentin-Bonding Agents; D011109:Polymethacrylic Acids; C113300:TPH hybrid; C444444:prime and bond 2.1; D000096:Acetone; D003094:Collagen; D012973:Sodium Hypochlorite