[Tissue specificity and expression of a human sperm protein's gene BSD-2.4].
OBJECTIVE
To investigate the tissue specificity and expression in spermatogenic stage of BSD-2.4 cDNA which might be related with fertility and its sequence was received by GenBank.


METHODS
Dot blot, Northern blot, and in situ hybridization were used.


RESULTS
Northern blot analysis of sixteen human tissues showed that BSD-2.4 cDNA had one transcript only with testis. BSD-2.4 mRNA was detected in germ cells of human testis.


CONCLUSIONS
BSD-2.4 is a testis-specific protein. The cDNA was expressed throughout the stages of the spermatogenic cycle.
Zhang
M
M
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Zhou
X
X
Cao
D
D
Miao
S
S
Wang
L
L
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013094:Spermatozoa / Q000737:chemistry*; D013737:Testis / Q000378:metabolism*
D018076:DNA, Complementary; D008565:Membrane Proteins; D012333:RNA, Messenger