title: [Study on immunomodulating mechanism of the active fraction of liuwei dihuang decoction].
abstract: OBJECTIVE
  To investigate the immunomodulating mechanism of 3A, an active fraction extracted from the ethanol-soluble portion of Liuwei Dihuang Decoction (LWDHD).
  
  
  METHODS
  Influence of 3A on T-lymphocyte subsets, IgG production of B cells, interferon-gamma (IFN-gamma) mRNA expression in cyclophosphamide (Cy)-treated mice, tumor bearing mice and senescence accelerated mouse-prone/8 (SAMP8) were assessed in vivo and in vitro.
  
  
  RESULTS
  Oral administration of 3A could elevate the decreased T-lymphocyte subsets in all model mice, it also could promote the IgG secretion of splenic cells in SAMP8 and increase IFN-gamma mRNA expression in Cy-treated mice.
  
  
  CONCLUSIONS
  The immunomodulating effect of 3A is related with its effects in regulating ratio of T and B cells or in improving their functions.
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Academy of Military Medicine, Beijing 100850.
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.forename: Y X
authors.initials: YX
authors.lastname: Zhang
authors.forename: X D
authors.initials: XD
authors.lastname: Lu
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D003520:Cyclophosphamide; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D016867:Immunocompromised Host / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012510:Sarcoma 180 / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D004365:Drugs, Chinese Herbal; D007074:Immunoglobulin G; C042579:Liuwei Dihuang Decoction; D012333:RNA, Messenger; D007371:Interferon-gamma; D003520:Cyclophosphamide