title: Evaluation of indices of skeletal muscle contraction in areas of referred hyperalgesia from an artificial ureteric stone in rats.
abstract: This study examined indices of skeletal muscle contraction in a rat model of referred muscle hyperalgesia from artificial ureteric calculosis [left oblique muscle (OE) for ipsilateral stone]. In specimens from the left versus right OE of stone-implanted female rats, a significant increase was found in membrane fluidity (P<0.01) and Ca(2+)-ATPase activity (P<0.0001) and a significant decrease in 3H-ryanodine binding (P<0.0001) and in I band length/sarcomere length ratio (contraction index) (P<0.01). The increase in Ca(2+)-ATPase activity was directly and significantly related to the number of rats' ureteral 'crises' (P<0.02). The results indicate a state of contraction in the hyperalgesic muscle, whose extent correlates to the algogenic activity of the ureteral stone.
authors.affiliation: Department of Medicine and Science of Aging, 'G D'Annunzio' University of, Chieti, Italy. mag@unich.it
authors.forename: Maria Adele
authors.initials: MA
authors.lastname: Giamberardino
authors.forename: Giannapia
authors.initials: G
authors.lastname: Affaitati
authors.forename: Rosanna
authors.initials: R
authors.lastname: Lerza
authors.forename: Giorgio
authors.initials: G
authors.lastname: Fanò
authors.forename: Stefania
authors.initials: S
authors.lastname: Fulle
authors.forename: Silvia
authors.initials: S
authors.lastname: Belia
authors.forename: Domenico
authors.initials: D
authors.lastname: Lapenna
authors.forename: Leonardo
authors.initials: L
authors.lastname: Vecchiet
mesh_terms: D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002462:Cell Membrane / Q000502:physiology; D005260:Female; D006930:Hyperalgesia / Q000503:physiopathology*; D008560:Membrane Fluidity / Q000502:physiology*; D008854:Microscopy, Electron; D023421:Models, Animal; D009119:Muscle Contraction / Q000502:physiology; D018482:Muscle, Skeletal / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012433:Ryanodine / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D014514:Ureteral Calculi / Q000473:pathology / Q000503:physiopathology*
chemical_list: D012433:Ryanodine; D017301:Ca(2+) Mg(2+)-ATPase