[Amplifying variable region gene of light chain of monoclonal antibody against human retinoblastoma by PCR].
OBJECTIVE
To acquire the variable region gene of light chain of monoclonal antibody against human retinoblastoma.


METHODS
Total RNA were extracted from hybridoma cells secreting specific monoclonal antibody(McAb) against human retinoblastoma(RB), then transcripted reversely into cDNA with random primers. The variable region of the light chain(VL) gene fragments were ampliflied using polymerase chain reaction(PCR) method. Agrose gel electrophoresis was confirmed.


RESULTS
1.5% agrose gel electrophoresis indicated that VL gene was about 340 base pairs.


CONCLUSIONS
The light chain variable region gene of the McAb against human RB was amplified successfully, which lays a good basis for construction of a recombinant antibody.
Zhong
X
X
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Li
Y
Y
Huang
S
S
Ge
J
J
Zhang
P
P
Zheng
J
J
Tang
S
S
Feng
G
G
D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000235:genetics; D000912:Antibodies, Neoplasm / Q000235:genetics*; D005784:Gene Amplification; D005803:Genes, Immunoglobulin / Q000235:genetics; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D016133:Polymerase Chain Reaction; D019572:Retinal Neoplasms / Q000235:genetics / Q000276:immunology; D012175:Retinoblastoma / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains