title: Melatonin effect on renal oxidative stress under constant light exposure.
abstract: Recently, numerous studies have shown antioxidant actions of melatonin. Melatonin at both physiological and pharmacological levels stimulates glutathione peroxidase, glutathione reductase and superoxide dismutase activities in the brains of rats and chickens. This study was designed to evaluate the effect of melatonin on nephropathy and oxidative stress under constant light exposure. Nephropathy was induced by adriamycin administered in a single dose (25 mg kg(-1) b.w., i.p.). Melatonin was injected i.p. (1,000 microg kg(-1) b.w./day). Malondialdehyde, reduced glutathione, glutathione peroxidase, glutathione reductase, glutathione transferase, catalase and superoxide dismutase were determined in kidney. Urea, creatinine and total proteins in plasma and proteinuria were evaluated and melatonin was determined. Results show a decrease in melatonin levels. Similar effects occurred with the antioxidant enzyme activities and reduced glutathione. Likewise, adriamycin and constant light induced significant enhancement of malondialdehyde. All changes induced both by adriamycin and constant light were reverted to normal by melatonin administration. Constant light exposure was associated with an increase in oxidative stress and nephropathy induced by adriamycin. Treatment with melatonin decreased lipid peroxides, and permitted a recovery of reduced glutathione, scavenger enzyme activity and parameters of renal function.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, University of Cordoba, Spain.
authors.forename: Isaac
authors.initials: I
authors.lastname: Túnez
authors.forename: María
authors.initials: M
authors.lastname: del Carmen Muñoz
authors.forename: Montserrat
authors.initials: M
authors.lastname: Feijoo
authors.forename: Manuel E
authors.initials: ME
authors.lastname: Valdelvira
authors.forename: Juan
authors.initials: J
authors.lastname: Rafael Muñoz-Castañeda
authors.forename: Pedro
authors.initials: P
authors.lastname: Montilla
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity; D000975:Antioxidants / Q000378:metabolism*; D004317:Doxorubicin / Q000633:toxicity; D006801:Humans; D007668:Kidney / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D007674:Kidney Diseases / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D008027:Light; D008297:Male; D008550:Melatonin / Q000378:metabolism / Q000494:pharmacology*; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D004317:Doxorubicin; D008550:Melatonin