title: Production of gamma-linolenic acid by disrupted mycelia of Mortierella isabellina.
abstract: OBJECTIVE
  To optimize the production of linolenic acid by disrupted mycelia of Mortierella isabellina.
  
  
  RESULTS
  Effects of incubation conditions such as incubation time, pH of reaction mixture, concentration of Mg2+ or malate and incubation temperature on production of linolenic acid were studied. The production of gamma-linolenic acid reached 224 mg g-1 dry cells when the reaction mixture was composed of 1.0 g (dry mycelial mass) of disrupted mycelia of M. isabellina, 50 ml (50 mmol l(-1)) potassium phosphate buffer supplemented with 0.312 mmol l(-1) of Mg2+ and 10 mmol l(-1) of malate, pH 7.0 and incubated at 5 degrees C for 1 day.
  
  
  CONCLUSIONS
  Incubation temperature, concentration of Mg2+ and malate showed major effects on the increased linolenic acid production.
  
  
  CONCLUSIONS
  This study highlights conditions for increasing gamma-linolenic acid production by cell-free mycelia of M. isabellina and an insight into rapidly gaining high production of polyunsaturated fatty acids.
authors.affiliation: Department of Chemistry, Jilin University, Changchun 130023, China. xianmo@263.net
authors.forename: M
authors.initials: M
authors.lastname: Xian
authors.forename: J
authors.initials: J
authors.lastname: Nie
authors.forename: Q
authors.initials: Q
authors.lastname: Meng
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: C
authors.initials: C
authors.lastname: Zhou
authors.forename: Y
authors.initials: Y
authors.lastname: Kang
authors.forename: K
authors.initials: K
authors.lastname: Zhen
mesh_terms: D003470:Culture Media; D044943:Fatty Acid Desaturases / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D020094:Mortierella / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D025282:Mycelium / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D013696:Temperature; D017965:gamma-Linolenic Acid / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D008293:Malates; D017965:gamma-Linolenic Acid; C030298:malic acid; D044943:Fatty Acid Desaturases; D008274:Magnesium