Glutaric acid induces oxidative stress in brain of young rats.
This study investigated the effects of glutaric acid, which predominantly accumulates in glutaric acidemia type I, on some in vitro parameters of oxidative stress in brain of young rats. We evaluated chemiluminescence, total radical-antioxidant potential (TRAP) and the activities of the antioxidant enzymes catalase, glutathione peroxidase and superoxide dismutase in brain tissue homogenates in the presence of glutaric acid at concentrations ranging from 0.05 to 2.0 mM. The acid significantly increased chemiluminescence (up to 65%) and reduced total radical-antioxidant potential (up to 28%) and glutathione peroxidase activity (up to 46%), without affecting catalase and superoxide dismutase activities. The results provide evidence that glutaric acid induces oxidative stress in vitro in rat brain. If these findings also occur in humans, it is possible that they may contribute to the neuropathology of patients affected by glutaric acidemia type I.
de Oliveira Marques
Fernanda
F
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 Anexo, Porto Alegre RS 90035-003, Brazil.
Hagen
Martine Elisabeth Kienzle
ME
Pederzolli
Carolina Didonet
CD
Sgaravatti
Angela Malysz
AM
Durigon
Karina
K
Testa
Carla Giordani
CG
Wannmacher
Clóvis Milton Duval
CM
de Souza Wyse
Angela Terezinha
AT
Wajner
Moacir
M
Dutra-Filho
Carlos Severo
CS
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000187:drug effects / Q000378:metabolism* / Q000503:physiopathology; D001928:Brain Diseases, Metabolic / Q000378:metabolism* / Q000503:physiopathology; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D005977:Glutarates / Q000378:metabolism* / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D008163:Luminescent Measurements; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions; D013482:Superoxide Dismutase / Q000378:metabolism
D005609:Free Radicals; D005977:Glutarates; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C035736:glutaric acid