Genetic analysis of Crimean-Congo hemorrhagic fever virus in Russia.
Genetic analysis of wild-type Crimean-Congo hemorrhagic fever (CCHF) virus strains recovered in the European part of Russia was performed. Reverse transcriptase PCR followed by direct sequencing was used to recover partial sequences of the CCHF virus medium (M) genome segment (M segment) from four pools of Hyalomma marginatum ticks and six human patients. Phylogenetic analysis of the M-segment sequences from Russian strains revealed a close relatedness of the strains (nucleotide sequence diversity, <or=5.0%). The strains differed significantly from CCHF viruses from other regions of the world (nucleotide sequence diversity, 10.3 to 20.4%), suggesting that CCHF virus strains recovered in the European part of Russia form a distinct group.
Yashina
Lyudmila
L
State Research Center of Virology and Biotechnology "Vector," Koltsovo, Novosibirsk Region, 630559, Russia.
Vyshemirskii
Oleg
O
Seregin
Sergei
S
Petrova
Irina
I
Samokhvalov
Evgeny
E
Lvov
Dmitry
D
Gutorov
Valery
V
Kuzina
Irina
I
Tyunnikov
Georgy
G
Tang
Yi-Wei
YW
Netesov
Sergei
S
Petrov
Vladimir
V
D016679:Genome, Viral; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000145:classification / Q000235:genetics*; D006479:Hemorrhagic Fever, Crimean / Q000635:transmission / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012426:Russia
D012367:RNA, Viral