title: Mapping quantitative trait loci associated with resistance to coccidiosis and growth.
abstract: To map QTL associated with disease resistance to avian coccidiosis and growth, two commercial broiler lines with different degrees of resistance to the disease were crossed to generate an F1 generation that was intercrossed to produce 314 F2 generation offspring. The F2 offspring were inoculated with sporulated oocysts of Eimeria maxima. Five disease-associated phenotypes were measured after the infection. Intertrait comparisons revealed that oocyst shedding was a good parameter for evaluating disease resistance or susceptibility. One hundred nineteen microsatellite markers, covering 80% of the chicken genome with an average marker interval of 25 cM, were used for genotyping of F1 parents and F2 offspring. Statistical analysis based on the data of four families revealed a locus on chromosome 1 associated with oocyst shedding (logarithm of odds = 3.46). The genetic mechanism of this locus appeared additive. The genomic scan also identified three potential growth QTL on chromosomes 1, 6, and 8. These results provide the foundation for further investigation to validate the QTL.
authors.affiliation: USDA-ARS, PBESL, Beltsville, Maryland 20705, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Zhu
authors.forename: H S
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: P C
authors.initials: PC
authors.lastname: Allen
authors.forename: C P
authors.initials: CP
authors.lastname: Van Tassell
authors.forename: T S
authors.initials: TS
authors.lastname: Sonstegard
authors.forename: H H
authors.initials: HH
authors.lastname: Cheng
authors.forename: D
authors.initials: D
authors.lastname: Pollock
authors.forename: M
authors.initials: M
authors.lastname: Sadjadi
authors.forename: W
authors.initials: W
authors.lastname: Min
authors.forename: M G
authors.initials: MG
authors.lastname: Emara
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics*; D002874:Chromosome Mapping; D003048:Coccidiosis / Q000235:genetics / Q000662:veterinary*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006128:Growth / Q000235:genetics*; D007113:Immunity, Innate / Q000235:genetics*; D008297:Male; D010641:Phenotype; D011201:Poultry Diseases / Q000235:genetics*; D040641:Quantitative Trait Loci