[Production and characterization of monoclonal antibody against the O antigen of Enterohemorrhagic Escherichia coli].
Spleen cells collected from Balb/c mice immunized with the somatic antigen of Enterohaemorrhagic E. coli O157:H7 are fused with murine Sp2/0 myeloma cells. A hybridoma cell line specific to E. coli O157 is established after screening and sub-cloning and is designated as 3A5. It belongs to a subtype of IgM and the ascetic titer of the antibody is 1:1 x 10(6). The detection limit is 10(5)-10(6) cfu/ml. This monoclonal antibody(McAb) also has activity with Enterohaemorrhagic E. coli O113:H21.
Zhao
Zhijing
Z
Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China.
Liu
Xiumei
X
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D019453:Escherichia coli O157 / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D019081:O Antigens / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D007106:Immune Sera; D007075:Immunoglobulin M; D019081:O Antigens