[Inhibitory effects of ginkgolide B on proliferation of bovine aortic smooth muscle cells].
OBJECTIVE
To investigate the effects of ginkgolide B (GB) on proliferation of bovine aortic smooth muscle cells (SMC) and its related mechanisms.


METHODS
After pretreating with GB or the mixture of ginkgolide A and B (GA:GB) at 37 degrees C for 0.5 h, the VSMC were treated with or without angiotensin II (Ang II) for 24 h. The proliferation of SMC was evaluated by 3H-thymidine incorporation and the cell cycle phase was measured by flow cytometry. Cytotoxicity was reflected by MTT and lactate dehydrogenase (LDH) activity of the supernatant.


RESULTS
Whether or not treated with Ang II, GB and GA:GB were shown to suppress SMC proliferation in concentration-dependent fashion at concentrations ranging from 10(-9) mol.L-1 to 10(-5) mol.L-1. The inhibitory effects appeared to be related to a G1-->S block in cell cycle traverse.


CONCLUSIONS
The suppression of SMC proliferation by GB might not only be contributed by blockage of the PAF receptor activity.
Wei
En-hui
EH
Department of Cardiovascular Pharmacology, Nanjing Medical University, Nanjing 210029, China.
Rao
Man-ren
MR
Ji
Ning-dong
ND
Chen
Xiu-ying
XY
Chen
Qi
Q
D000818:Animals; D001011:Aorta / Q000166:cytology; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D016193:G1 Phase / Q000187:drug effects; D046934:Ginkgolides; D007783:Lactones / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D010980:Platelet Membrane Glycoproteins / Q000037:antagonists & inhibitors; D043562:Receptors, G-Protein-Coupled / Q000037:antagonists & inhibitors
D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D010980:Platelet Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor; D004247:DNA; C045856:ginkgolide B