title: [Cloning of the major antigen region of E2 gene of hog cholera virus and expression in Escherichia coli].
abstract: The major antigen region of E2 gene of Hog Cholera Prevalent Strain (Guangxi Yuling Strain) and Chinese Hog Cholera Lapinised Virus (C-strain) derived from hog and rabbit spleen tissue, was amplified by reverse transcription polymerase chain reaction(RT-PCR) and the nested Polymerase Chain Reaction (nPCR). After the amplified fragments were cloned into the expression vector pPROEX-HTb, the recombinant plasmids pPROEX-GXYL and pPROEX-C were obtained. The insert position, the size and the reading frame were right by PCR, restriction digestion and the sequence analysis. SDS-PAGE indicated that both of the reciepient germs transducted and induced by the recombinant plasmids pPROEX-GXYL and pPROEX-C could express the major antigen region of E2 gene. Western-blot indicated that the expressed antigen protein could be recognized by the positive serum of CSFV.
authors.affiliation: Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China.
authors.forename: Yong-Guo
authors.initials: YG
authors.lastname: Zhang
authors.forename: Xiang-Tao
authors.initials: XT
authors.lastname: Liu
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Han
authors.forename: Xi-Cheng
authors.initials: XC
authors.lastname: Liu
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Zhang
authors.forename: Qing-Ge
authors.initials: QG
authors.lastname: Xie
mesh_terms: D015153:Blotting, Western; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology
chemical_list: D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C087086:glycoprotein E2, classical swine fever virus