[Influence of cell cycle in human trabecular cells induced by dexamethsone].
OBJECTIVE
To investigate the influence of dexamethesone (Dex) on cell cycle in human trabecular cells(HTC).


METHODS
The trabecular specimens from human eyes were cultured in vitro. Then the cultured cells were immersed in DMEM solution containing 10(-7) M dexamethesone for three, five and seven days. The cell cycle was detected by Flow Cycometry.


RESULTS
The G2 phase of cell cycle in HTC increased when HTC were treated by Dex. The G2 phase was 2% in control group. For three, five and seven days treated by Dex, the G2 phase were 8%, 16.3 and 19.4%.


CONCLUSIONS
The influence of cell cycle might be induced by Dex, it may be related with the pathogenesis of glucocorticoid induced glaucoma.
Zhou
Y
Y
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Ge
J
J
Lin
M
M
Lan
Y
Y
Zheng
J
J
D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003907:Dexamethasone / Q000009:adverse effects / Q000494:pharmacology*; D016195:G2 Phase / Q000187:drug effects; D005901:Glaucoma / Q000139:chemically induced; D006801:Humans; D014129:Trabecular Meshwork / Q000166:cytology*
D003907:Dexamethasone