Placental glucose transport in growth-restricted pregnancies induced by overnourishing adolescent sheep.
Glucose clamp procedures were used to determine whether the slowing of fetal growth during the final third of gestation in overnourished adolescent ewes is due to a reduction in placental glucose transport capacity. Singleton pregnancies to a single sire were established by embryo transfer and thereafter adolescent dams were offered a high (n = 11) or moderate (n = 7) nutrient intake. Studies were conducted at 130 +/- 0.5 days gestation. Uterine and umbilical blood flows were studied by the steady-state transplacental diffusion technique and glucose fluxes quantified by the Fick principle. To determine the relationship between the transplacental glucose gradient and umbilical (fetal) glucose uptake, studies were conducted with maternal arterial glucose clamped at 5 micromol ml(-1) and fetal glucose at spontaneously occurring and two additional higher levels. Maternal body weight gain during gestation averaged 282 and 57 g day(-1) for high- and moderate-intake dams, respectively. Total placentome weight (209 +/- 23 vs. 386 +/- 34 g) and fetal weight (3072 +/- 266 vs. 4670 +/- 196 g) were lower (P < 0.001) in high- than in moderate-intake groups. The growth-restricted pregnancies in the high-intake dams were associated with reduced uterine (P < 0.05) and umbilical (P < 0.02) blood flows and, in the non-perturbed state, the fetuses were relatively hypoxic (2.1 vs. 3.0 micromol ml(-1), P < 0.05) and hypoglycaemic (0.90 vs. 1.31 micromol ml(-1), P < 0.002). Linear regression analysis of umbilical glucose uptake at three steady-state uterine-umbilical arterial transplacental plasma glucose concentration gradients revealed that absolute placental glucose transport capacity was lower in high- than in moderate-intake dams (mean slope, 0.8 vs. 1.5 dl min(-1), P < 0.05; and mean intercept, 1.84 vs. 3.40 micromol ml(-1)). However, glucose transfer capacity was not different between the two groups when expressed on a placental weight-specific basis. This confirms that the small size of the placenta per se is the major limitation to placental glucose transfer in the overnourished adolescent pregnant sheep.
Wallace
Jacqueline M
JM
The Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK. jacqueline.wallace@rri.sari.ac.uk
Bourke
Deirdre A
DA
Aitken
Raymond P
RP
Milne
John S
JS
Hay
William W
WW
D000367:Age Factors; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D001835:Body Weight / Q000502:physiology; D005260:Female; D005317:Fetal Growth Retardation / Q000378:metabolism* / Q000503:physiopathology; D007003:Hypoglycemia / Q000378:metabolism / Q000503:physiopathology; D008431:Maternal-Fetal Exchange / Q000502:physiology*; D010100:Oxygen / Q000097:blood; D010920:Placenta / Q000378:metabolism*; D010927:Placental Insufficiency / Q000378:metabolism / Q000503:physiopathology; D011247:Pregnancy; D011256:Pregnancy Outcome; D039401:Prenatal Nutritional Physiological Phenomena / Q000502:physiology*; D014470:Umbilical Cord / Q000098:blood supply; D014599:Uterus / Q000098:blood supply
D001786:Blood Glucose; D010100:Oxygen