[Preparation of monoclonal antibodies by intrasplenic immunization of mice with urine circulating antigen from patients infected with Schistosoma japonicum].
OBJECTIVE
To explore an efficient method for preparing monoclonal antibodies against circulating antigen of S. japonicum in the urine of schistosomiasis patients.


METHODS
The urine of schistosomasis patients were precipitated with 5% trichloroacetic acid, and then used to immunize the BALB/c mice by intrasplenic immunization to prepare McAb.


RESULTS
Two monoclonal cell lines against the CAg in the urine of schistosomiasis patients have been established. The immunoglobulin subclass of the 2 McAbs (2E6 and 2B11) were identified as IgM. The titres of 2E6 and 2B11 were 2.56 x 10(5) and 6.4 x 10(4). On immunodiffusion, McAb 2E6 showed one precipitation line with IHU-CAg, however, both McAbs did not react with NHU-CAg. Using ascitic fluid of 2B11 and 2E6 as capture antibody separately, and HRP-H11 as labelled antibody to detect the urine of 12 acute schistosomasis patients, 2B11 2E6 gave 6 and 3 positive, respectively, whereas 8 normal urine were all negative.


CONCLUSIONS
It is feasible to use CAg from schistosomasis patients' urine to prepare anti-CAg McAb by intrasplenic immunization in mice.
Wu
C
C
Department of Parasitology, Shanghai Secand Medical University, Shanghai 200025.
Lou
W
W
Xue
C
C
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology / Q000652:urine*; D002460:Cell Line; D005260:Female; D006801:Humans; D007114:Immunization; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis*; D013154:Spleen
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth