title: [Expression of GATA-2 Gene and Immunoglobulin Heavy Chain Germline Gene C( micro ) in Leukemia Cells and Its Significance]
abstract: To investigate the significance of GATA-2 and immunoglobulin heavy chain germline gene C( micro ) (IgH germline gene C( micro )) expression and coexpression in various leukemia cells, GATA-2 and IgH germline gene C( micro ) mRNA in bone marrow and peripheral blood cells from 63 leukemia patients were detected by reverse transcription-polymerase chain reaction (RT-PCR). No GATA-2 or IgH germline gene C( micro ) mRNA were detected in normal bone marrow and peripheral blood. GATA-2 mRNA were be detected in 91.3% patients with acute myeloid leukemia (AML), 75% patients with acute lymphoblastic leukemia (ALL) as well as 83.3% patients with chronic myeloid leukemia (CML-CP); IgH germline gene C( micro ) mRNA were be identified in 47.8% AML, 41.6% ALL, as well as 5.6% CML-CP. All patients with CML-AP and CML-BC expressed GATA-2 mRNA and partly expressed IgH germline gene C( micro ) mRNA. 47.8% AML and 41.6% ALL patients coexpressed GATA-2 and IgH germline gene C( micro ) mRNA. GATA-2(+) IgH germline gene C( micro )(+) cells of AML and ALL were mainly HLA-DR positive. As aberration of the transcription factors, GATA-2 and germline IgH germline gene C( micro ) gene might been linked to leukemogenesis. Various expression of GATA-2 and germline IgH germline gene C( micro ) gene in leukemia might correlated with the heterogeneous differentiation level of leukemia cells. The fact that leukemia with GATA-2(+) IgH germline gene C( micro )(+) coexpression indicated multilineage impairment of hematopoietic cells.
authors.affiliation: Center of Bone Marrow Transplaontation, Beijing Beitaiping Road Hospital, Beijing 100039, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Lujia
authors.initials: L
authors.lastname: Dong
authors.forename: Guangxian
authors.initials: G
authors.lastname: Liu
authors.forename: Liangding
authors.initials: L
authors.lastname: Hu
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Shuqiong
authors.initials: S
authors.lastname: Jia
authors.forename: Shujuan
authors.initials: S
authors.lastname: Lu
authors.forename: Hu
authors.initials: H
authors.lastname: Chen