title: Fibroblast growth factor receptor 3 signaling regulates the onset of oligodendrocyte terminal differentiation.
abstract: Fibroblast growth factor receptor (FGFR) signaling is essential for nervous system development. We have shown that, in the normal postnatal brain, the spatial and temporal expression pattern of FGFR3 parallels the appearance of differentiated oligodendrocytes and that in culture FGFR3 is expressed maximally at the critical stage in the lineage at which oligodendrocyte late progenitors (Pro-OLs) enter terminal differentiation. Therefore, FGFR3 expression is positioned ideally to have an impact on oligodendrocyte differentiation. In support of this we show that, during the onset and active phase of myelination in FGFR3-deficient mice, there are reduced numbers of differentiated oligodendrocytes in the forebrain, cerebellum, hindbrain, and spinal cord. Furthermore, myelination is delayed in parallel. Delay of oligodendrocyte differentiation also is observed in primary cell culture from this mutant. On the other hand, no differences are observed in the survival or proliferation of oligodendrocyte progenitors. This suggests that the decrease in the number of differentiated oligodendrocytes is attributable to a delay in the timing of their differentiation process. Astrocytes also express FGFR3, and in mice lacking FGFR3 there is an enhancement of the astrocytic marker glial fibrillary acidic protein expression in a region-specific manner. Thus our findings suggest that there are cell type- and region-specific functions for FGFR3 signaling and in particular emphasize a prominent role for FGFR3 as part of a system regulating the onset of oligodendrocyte terminal differentiation.
authors.affiliation: Department of Neuroscience, University of Connecticut Medical School, Farmington, Connecticut 06030-3401, USA.
authors.forename: Luke Y S
authors.initials: LY
authors.lastname: Oh
authors.forename: Adam
authors.initials: A
authors.lastname: Denninger
authors.forename: Jennifer S
authors.initials: JS
authors.lastname: Colvin
authors.forename: Aditee
authors.initials: A
authors.lastname: Vyas
authors.forename: Shubha
authors.initials: S
authors.lastname: Tole
authors.forename: David M
authors.initials: DM
authors.lastname: Ornitz
authors.forename: Rashmi
authors.initials: R
authors.lastname: Bansal
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D001369:Axons / Q000648:ultrastructure; D001921:Brain / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D002452:Cell Count; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D015536:Down-Regulation; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D018345:Mice, Knockout; D009186:Myelin Sheath / Q000378:metabolism / Q000648:ultrastructure; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases; D012333:RNA, Messenger / Q000096:biosynthesis; D051498:Receptor, Fibroblast Growth Factor, Type 3; D017468:Receptors, Fibroblast Growth Factor / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; C496366:Fgfr3 protein, mouse; D011505:Protein-Tyrosine Kinases; D051498:Receptor, Fibroblast Growth Factor, Type 3