title: [Quantitation of telomerase activity using the polymerase chain reaction-based enzyme immunoassay and its clinical application].
abstract: OBJECTIVE
  To investigate detective methodology of telomerase activity and its clinical application.
  
  
  METHODS
  The telomerase activity was quantitated by polymerase chain reaction-base enzyme immunoassay (PCR-EIA). The TS primer was biotinylated (TS-B), and CX primer was digoxigeninated (CX-D). The amplicons containing TS-B were combined with microtiter plate coated streptavidin, then combined with anti-digoxigenin antibody labeled with POD and finally reacted to tetramethylbenzidine substrate solution.
  
  
  RESULTS
  Telomerase activity measured by the PCR-EIA method was comparable to that obtained from TRAP-silver stain protocol. The CV of the PCR-EIA method was 4.138%. Telomerase activity was detected in 90% of various tumer tissues. In control tissues, telomerase activity was detected only in 7.1%.
  
  
  CONCLUSIONS
  The PCR-EIA method offers a rapid, quantitative, and nonisotopic assay for the determination of telomerase activity. It is simpler than silver stain protocol. The detection of telomerase activity may play a significant role in the diagnosis of clinical tumors.
authors.affiliation: Department of Clinical Biochemistry, Xiangya School of Medicine, Central South University, Changsha 410013, China.
authors.forename: Wei-qun
authors.initials: WQ
authors.lastname: Chen
authors.forename: He-ming
authors.initials: HM
authors.lastname: Chen
authors.forename: Yuan-jia
authors.initials: YJ
authors.lastname: Hu
mesh_terms: D015179:Colorectal Neoplasms / Q000201:enzymology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D008175:Lung Neoplasms / Q000201:enzymology; D009303:Nasopharyngeal Neoplasms / Q000473:pathology; D009369:Neoplasms / Q000201:enzymology*; D016133:Polymerase Chain Reaction / Q000379:methods; D019098:Telomerase / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D019098:Telomerase