title: Population survey of CCR5 delta32, CCR5 m303, CCR2b 64I, and SDF1 3'A allele frequencies in indigenous Chinese healthy individuals, and in HIV-1-infected and HIV-1-uninfected individuals in HIV-1 risk groups.
abstract: The aim of this study is to determine in indigenous Chinese ethnic groups the frequencies of the chemokine (SDF1 3'A) and chemokine receptors (CCR5 delta32, CCR5 m303, and CCR2b 64I) HIV-1/AIDS restriction alleles. The study includes two cohorts; the first comprised 3165 indigenous healthy subjects representing eight ethnic groups: Han (n = 1406), Uygur (n = 316), Mongolia (n = 134), Hui (n = 386), Tibetan (n = 330), Zhuang (n = 378), Dai (n = 101), and Jingbo (n =114). The second cohort consisted of 330 HIV-1-infected (86 subjects infected by sexual transmission and 198 subjects infected by HIV-1-contaminated blood or by sharing injection equipment; the remaining 46 subjects said nothing about HIV-1 transmission) and 474 HIV-1-uninfected Han Chinese belonging to one of two HIV-1 high-risk groups: intravenous drug users (n = 215) and individuals with sexually transmitted diseases (n = 259). Genotypes for the four genes were obtained using PCR (CCR5 delta32) or PCR-restriction fragment length polymorphism. Randomly selected amplified PCR products were further confirmed by direct DNA sequencing. The variant allele frequencies were determined to be 0% to 3.48% for CCR5 delta32, 0% for CCR5 m303, 16.23% to 28.79% for CCR2b 64I, and 17.70% to 27.76% for SDF1 3'A in Chinese healthy individuals from eight ethnic groups. These findings show that allele frequencies differ among the eight Chinese ethnic groups for CCR5 delta32, CCR2b 64I, and SDF1 3'A and that the CCR5 m303 and CCR5 delta32 mutant alleles were absent or infrequent in Chinese, which may be helpful for studies of specific anti-HIV-1 vaccine trials and coreceptor inhibitor drug targets in Chinese populations. Furthermore, we observed no significant differences in allele or genotypic frequencies between HIV-1-infected and HIV-1-uninfected groups from the Han ethnic group. Our finding is the first reporting that there is likely no effect of the examined polymorphisms in our study on HIV-1 transmission in the Chinese Han population, However, the genetic effects of these and other AIDS-modifying polymorphisms on the pathogenesis and clinical outcome of HIV-1/AIDS diseases is under investigation in Chinese populations.
authors.affiliation: Division of Biological Engineering, Beijing Institute of Infectious Disease, Beijing Hospital of Infectious Disease, Beijing, China. fswang@public.bta.net.cn
authors.forename: Fu-Sheng
authors.initials: FS
authors.lastname: Wang
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Hong
authors.forename: Yunzhen
authors.initials: Y
authors.lastname: Cao
authors.forename: Ming-Xu
authors.initials: MX
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Jin
authors.forename: Liang-Ping
authors.initials: LP
authors.lastname: Hu
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Tie-Jian
authors.initials: TJ
authors.lastname: Feng
authors.forename: Jing
authors.initials: J
authors.lastname: Hou
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Shi
authors.forename: Dong-Ping
authors.initials: DP
authors.lastname: Xu
authors.forename: Zhou-Yun
authors.initials: ZY
authors.lastname: Lei
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Zheng-Dong
authors.initials: ZD
authors.lastname: Liu
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Ye
authors.forename: Lin
authors.initials: L
authors.lastname: Peng
authors.forename: Ya
authors.initials: Y
authors.lastname: Qiu
authors.forename: Cheryl
authors.initials: C
authors.lastname: Winkler
mesh_terms: D000328:Adult; D018932:Chemokine CCL2; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000235:genetics*; D002681:China / Q000208:ethnology; D015331:Cohort Studies; D018562:Disease Transmission, Infectious; D005006:Ethnicity; D005787:Gene Frequency; D005820:Genetic Testing; D005838:Genotype; D015658:HIV Infections / Q000097:blood / Q000276:immunology* / Q000635:transmission; D015497:HIV-1; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D011159:Population Surveillance; D054390:Receptors, CCR2; D019713:Receptors, CCR5 / Q000235:genetics*; D019707:Receptors, Chemokine / Q000235:genetics*; D012307:Risk Factors; D015819:Substance Abuse, Intravenous / Q000097:blood / Q000276:immunology
chemical_list: C515334:CCR2 protein, human; C515255:CXCL12 protein, human; D018932:Chemokine CCL2; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D054390:Receptors, CCR2; D019713:Receptors, CCR5; D019707:Receptors, Chemokine