title: [Establishment of a Human Umbilical Vein Endothelial Cell Line Supporting Hematopoiesis In Vitro]
abstract: Endothelial cells are the critical cell component of hematopoietic microenvironment. For the purpose of facilitating the study on modulating effect of endothelial cells in hematopoiesis, a human umbilical vein cell line, IEC, was established. Primary human umbilical vein endothelial cells were transfected with the plasmid pSV(3neo) carried SV40 large T antigen by lipofection. The IEC cells expressed factor VIII and the UEA I-binding ratio was about 97%. The chromosome variation was existed in the cell line, with the karyotype 45, XX, -18, 18q(+). The cell line retained the proliferative capacity at least 25 passages. IEC cells stimulated the growth of granulocyte-macrophage progenitor cells in coculture of cord blood CD34(+) cells with IEC cells. It is concluded that IEC cell line possessed the biological features of endothelial cell and supported hematopoiesis in vitro.
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Hong-Feng
authors.initials: HF
authors.lastname: Yuan
authors.forename: Zi-Kuan
authors.initials: ZK
authors.lastname: Guo
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Liu
authors.forename: Shuang-Xi
authors.initials: SX
authors.lastname: Zhang
authors.forename: Ning
authors.initials: N
authors.lastname: Mao