title: [Cytogenetic analysis of two ecdysone-regulated puffs 62C and 62E in Drosophila melanogaster].
abstract: Genetic analysis has been performed to reveal vital genes around two puffs, a late 62C puff and an early-late 62E puff. Their roles in hormonal regulatory mechanisms have been estimated. A locus represented by four lethal mutations has been found in the vicinity of the 62E puff. The mutants display disturbed puffing, which suggests the involvement of this locus in hormonal regulatory mechanisms. In the 62C puff region. 26 mutations have been found that proved to be allelic to mutations in the D-Titin gene. The giant D-Titin gene is essential for the sarcomeric organization of striated muscles. According to the results of in situ hybridization with polytene chromosomes, the D-Titin gene occupies the entire 62C puff. The phenotypic characteristics of the novel mutants suggest that this protein is polyfunctional, and its role is not restricted to processes in the muscular tissue. It may also be involved in the morphogenesis of leg imaginal disks, and it is necessary for condensation and separation of sister chromatids during mitosis. Mutations in the ecdysone-induced BR-C and E74 genes cause disturbances similar to those found in this study. In addition, mutations of these genes can affect the D-Titin gene activity, which suggests that the three genes are involved in similar morphogenetic and myogenetic processes.
authors.affiliation: Institute of Cytology and Genetics, Siberian Division, Russian Academy of Sciences, Novosibirsk, 630090 Russia. galina@bionet.nsc.ru
authors.forename: G V
authors.initials: GV
authors.lastname: Pokholkova
authors.forename: I F
authors.initials: IF
authors.lastname: Zhimulev
mesh_terms: D000818:Animals; D002875:Chromosomes / Q000502:physiology*; D064211:Connectin; D020732:Cytogenetic Analysis; D004268:DNA-Binding Proteins / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D004440:Ecdysone / Q000378:metabolism*; D005121:Extremities / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D005804:Genes, Lethal; D019476:Insect Proteins / Q000235:genetics; D007814:Larva; D008675:Metamorphosis, Biological; D009124:Muscle Proteins / Q000235:genetics; D009154:Mutation; D014157:Transcription Factors / Q000235:genetics
chemical_list: C102117:Br protein, Drosophila; D064211:Connectin; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C060408:Eip74EF protein, Drosophila; D019476:Insect Proteins; D009124:Muscle Proteins; D014157:Transcription Factors; C577347:sls protein, Drosophila; D004440:Ecdysone