title: Characterization of a new integron containing VIM-2, a metallo- beta-lactamase gene cassette, in a clinical isolate of Enterobacter cloacae.
abstract: We report the first description of a new integron containing bla(VIM-2), a metallo-beta-lactamase gene from Enterobacter cloacae KU680, which was isolated from peritoneal fluid of a liver cirrhosis patient in South Korea. Antibiotic susceptibility testing, and modified Hodge and EDTA-disc synergy tests, were carried out to screen for metallo-beta-lactamase-producing strains. PCR and sequence analysis were used to identify and analyse the bla(VIM-2)-containing integron. The isolate was resistant to most beta-lactams, including imipenem, and demonstrated a positive modified Hodge and EDTA-disc synergy test, which are findings suggesting a metallo-beta-lactamase. Preliminary PCR-based experiments detected the metallo-beta-lactamase gene bla(VIM-2). Sequencing of the 4392 bp cloned PCR amplicon, containing the gene cassette bla(VIM-2), revealed the structure of the class 1 integron. The integron also contained additional insert gene cassettes, aadA, and unknown open reading frames 'orfII' and 'orfIII'. To the best of our knowledge, this is the first time that this metallo-beta-lactamase gene has been detected in E. cloacae.
authors.affiliation: Departments of Clinical Pathology, Internal Medicine and Therapeutic Radiology, Kosin University College of Medicine, Amnam-Dong 34, Seo-Ku, Busan, South Korea. kscpjsh@hs.kosinmed.or.kr
authors.forename: Seok Hoon
authors.initials: SH
authors.lastname: Jeong
authors.forename: Kyungwon
authors.initials: K
authors.lastname: Lee
authors.forename: Yunsop
authors.initials: Y
authors.lastname: Chong
authors.forename: Jong Hwa
authors.initials: JH
authors.lastname: Yum
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Hyuk Jin
authors.initials: HJ
authors.lastname: Choi
authors.forename: Jeong Man
authors.initials: JM
authors.lastname: Kim
authors.forename: Kil Ho
authors.initials: KH
authors.lastname: Park
authors.forename: Byung Hoon
authors.initials: BH
authors.lastname: Han
authors.forename: Sang Wook
authors.initials: SW
authors.lastname: Lee
authors.forename: Tae Sik
authors.initials: TS
authors.lastname: Jeong
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D016972:Enterobacter cloacae / Q000201:enzymology / Q000235:genetics*; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D032023:Integrons / Q000235:genetics*; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: C425108:beta-lactamase bla(vim-2); D001618:beta-Lactamases