[Study on tissue culture of Poligonum multiflorum].
In the present thesis, suitable medium for callus inducement and relationship between culture time and callus growth of Poligonum multiflorum were studied by mono-factorial and orthogonal design test. The experiments showed that the best medium for callus inducement was MS + 2,4-D 1 mg/L + 6-BA 1 mg/L + IBA 0.5 mg/L, dark culture was better than light culture, callus grew fast when it was cultured 12 days, whole plant could be induced by callus, the induce rate was 95%.
Du
Q
Q
Guangzhou University of TCM, Guangzhou 510407.
Fu
H
H
Zhan
R
R
Yao
Y
Y
Li
X
X
D003470:Culture Media; D046508:Culture Techniques; D006728:Hormones; D008027:Light; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000254:growth & development*; D019664:Polygonaceae / Q000254:growth & development*
D003470:Culture Media; D006728:Hormones; D010937:Plant Growth Regulators