title: Simple and rapid determination of thiabendazole, imazalil, and o-phenylphenol in citrus fruit using flow-injection electrospray ionization tandem mass spectrometry.
abstract: A simple and rapid analytical method for thiabendazole (TBZ), imazalil (IMA), and o-phenylphenol (OPP) in citrus fruit has been developed by using flow-injection electrospray ionization tandem mass spectrometry for the first time. The method involves the combined use of stable isotopically labeled internal standards (thiabendazole-(13)C(6), imazalil-d(5), and p-phenylphenol-d(9)) and a multiple reaction monitoring technique. The average recoveries for the fungicides at the tolerance levels (TBZ and OPP, 10 mg/kg; IMA, 5 mg/kg) ranged from 77 to 101%, with the coefficients of variation (CVs) ranging from 0.7 to 4.2% (n = 5). At half the tolerance levels (TBZ and OPP, 5 mg/kg; IMA, 2.5 mg/kg), the average recoveries ranged from 62 to 112%, with the CVs ranging from 0.7 to 8.4% (n = 5). The CVs of the average recoveries, obtained from lemon samples fortified with three fungicides at the tolerance levels, obtained on three different days over two weeks, ranged within 2%. The analysis time, including sample preparation and determination, is only 15 min.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan. yuuko_1_itou@pref.aichi.lg.jp
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ito
authors.forename: Tomomi
authors.initials: T
authors.lastname: Goto
authors.forename: Hisao
authors.initials: H
authors.lastname: Oka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Takahashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakazawa
mesh_terms: D001713:Biphenyl Compounds / Q000032:analysis; D002957:Citrus / Q000737:chemistry*; D017022:Flow Injection Analysis; D005638:Fruit / Q000737:chemistry*; D005659:Fungicides, Industrial / Q000032:analysis*; D007093:Imidazoles / Q000032:analysis; D011786:Quality Control; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000592:standards; D013827:Thiabendazole / Q000032:analysis
chemical_list: D001713:Biphenyl Compounds; D005659:Fungicides, Industrial; D007093:Imidazoles; C017435:enilconazole; C004369:2-phenylphenol; D013827:Thiabendazole