title: Effects of lysogeny of Shiga toxin 2-encoding bacteriophages on pulsed-field gel electrophoresis fragment pattern of Escherichia coli K-12.
abstract: Escherichia coli K-12 lysogens of three different Shiga toxin 2 (Stx2)-encoding bacteriophages were examined for variability in their pulsed-field gel electrophoresis (PFGE) fragment patterns. The PFGE fragment patterns could be classified into three types (i.e., PFGE types B, C, and D). For the PFGE type D, a 255-kbp fragment present in the original K-12 strain was apparently shifted by the size of Stx 2-encoding phage genomic DNA (ca. 65 kbp) to the position at 320 kbp. In contrast, the types B and C showed the above fragment shift plus further 6- and 10-fragment differences, respectively, from the original K-12 strain. The evidence suggests that even a single genetic event like lysogeny can cause marked genotypic modification of the host strain.
authors.affiliation: Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Iguchi
authors.forename: Ro
authors.initials: R
authors.lastname: Osawa
authors.forename: Junichi
authors.initials: J
authors.lastname: Kawano
authors.forename: Akira
authors.initials: A
authors.lastname: Shimizu
authors.forename: Jun
authors.initials: J
authors.lastname: Terajima
authors.forename: Haruo
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D001435:Bacteriophages / Q000235:genetics* / Q000254:growth & development; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000821:virology*; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification; D008242:Lysogeny; D022641:Shiga Toxin 2 / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D022641:Shiga Toxin 2