title: Biosynthetic response of passaged chondrocytes in a type II collagen scaffold to mechanical compression.
abstract: To investigate the potential utility of mechanical loading in articular cartilage tissue engineering, porous type II collagen scaffolds seeded with adult canine passaged chondrocytes were subjected to static and dynamic compressions of varying magnitudes (0-50% static strain) and durations (1-24 h), and at different times during culture (2-30 days postseeding). The effects of mechanical compression on the biosynthetic activity of the chondrocytes were evaluated by measuring the amount of (3)H-proline-labeled proteins and (35)S-sulfate-labeled proteoglycans that accumulated in the cell-scaffold construct and was released to the medium during the loading period. Similar to published results on loading of articular cartilage explants, static compression decreased protein and proteoglycan biosynthesis in a time- and dose-dependent manner (each p < 0.005), and selected dynamic compression protocols were able to increase rates of biosynthesis (p < 0.05). The main difference between the results seen for this tissue engineering system and cartilage explants was in the amount of newly synthesized matrix molecules that accumulated within the construct under dynamic loading, with less accumulating in the type II collagen scaffold. In summary, the general biosynthetic response of passaged chondrocytes in the porous type II collagen scaffolds is similar to that seen for chondrocytes in their native environment. Future work needs to be directed to modifications of the cell-seeded construct to allow for the capture of the newly synthesized matrix molecules by the scaffold.
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: C R
authors.initials: CR
authors.lastname: Lee
authors.forename: A J
authors.initials: AJ
authors.lastname: Grodzinsky
authors.forename: M
authors.initials: M
authors.lastname: Spector
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D001696:Biomechanical Phenomena; D002358:Cartilage, Articular / Q000166:cytology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D024043:Collagen Type II / Q000378:metabolism*; D019245:Compressive Strength; D004285:Dogs; D008422:Materials Testing; D011392:Proline / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D013314:Stress, Mechanical; D013431:Sulfates / Q000378:metabolism; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D024043:Collagen Type II; D011506:Proteins; D011509:Proteoglycans; D013431:Sulfates; D011392:Proline