Hemin binding, functional expression, and complementation analysis of Pap 31 from Bartonella henselae.
Growth of Bartonella henselae is strongly heme dependent, and B. henselae is unable to synthesize heme itself. At least five outer membrane-associated proteins from B. henselae bind hemin, including the 31-kDa protein designated Pap31. The gene of this protein was heterologously expressed in Escherichia coli M15(pREP4) and detected with monoclonal antibodies in the outer membrane fraction. Complementation of the hemA-deficient mutant E. coli K-12 EB53 (aroB tsx malT hemA) with pap31 demonstrated that this protein is involved in heme acquisition and may be an important virulence factor in the pathogenesis of B. henselae.
Zimmermann
Rainer
R
Institute of Medical Microbiology and Hygiene, University of Freiburg, Freiburg, Germany.
Kempf
Volkhard A J
VA
Schiltz
Emile
E
Oberle
Karin
K
Sander
Anna
A
D000911:Antibodies, Monoclonal / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D018416:Bartonella henselae / Q000235:genetics*; D001483:Base Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000235:genetics; D005816:Genetic Complementation Test; D006427:Hemin / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; C110491:Pap31 protein, Bartonella henselae phage 60457; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014764:Viral Proteins; D006427:Hemin