title: Versatility of inner membrane protein biogenesis in Escherichia coli.
abstract: To further our understanding of inner membrane protein (IMP) biogenesis in Escherichia coli, we have accomplished the widest in vivo IMP assembly screen so far. The biogenesis of a set of model IMPs covering most IMP structures possible has been studied in a variety of signal recognition particle (SRP), Sec and YidC mutant strains. We show that the assembly of the complete set of model IMPs is assisted (i.e. requires the aid of proteinaceous factors), and that the requirements for assembly of the model IMPs into the inner membrane differ significantly from each other. This indicates that IMP assembly is much more versatile than previously thought.
authors.affiliation: Department of Biochemistry and Biophysics, Arrhenius Laboratories, Stockholm University, SE-106 91 Stockholm, Sweden.
authors.forename: Linda
authors.initials: L
authors.lastname: Fröderberg
authors.forename: Edith
authors.initials: E
authors.lastname: Houben
authors.forename: James C
authors.initials: JC
authors.lastname: Samuelson
authors.forename: Minyong
authors.initials: M
authors.lastname: Chen
authors.forename: Sei-Kyoung
authors.initials: SK
authors.lastname: Park
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Phillips
authors.forename: Ross
authors.initials: R
authors.lastname: Dalbey
authors.forename: Joen
authors.initials: J
authors.lastname: Luirink
authors.forename: Jan-Willem L
authors.initials: JW
authors.lastname: De Gier
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001483:Base Sequence; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D026901:Membrane Transport Proteins / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D018271:Signal Recognition Particle / Q000096:biosynthesis / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D000069816:SEC Translocation Channels; D018271:Signal Recognition Particle; C412443:YIDC protein, E coli; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins