title: Viral respiratory infections in young children attending day care in urban Northeast Brazil.
abstract: A cohort of children attending a day care center in Salvador (Bahia, Brazil) was studied prospectively to determine the incidence of viral respiratory infectious episodes and to identify the viruses associated with them. Two hundred seventy-one nasopharyngeal samples were collected over a 1-year period for examination, using indirect immunofluorescence with monoclonal antibodies against adenovirus, influenza A and B, parainfluenzae 1-3, and respiratory syncytial virus, and reverse transcriptase-polymerase chain reaction for picornavirus. Examination yielded positive results in 116 samples (42.8%). Rhinovirus was identified alone in 56 samples (48.3%) and was observed along with other viruses in 11 additional samples. Incidence density of viral respiratory infectious episodes was 7.66 episodes/1,000 child-days.
authors.affiliation: Department of Pediatrics and Department of Preventive Medicine, Faculty of Medicine Federal University of Bahia, Salvador, Bahia, Brazil. ledasolano@bol.com.br
authors.forename: Leda Solano de Freitas
authors.initials: LS
authors.lastname: Souza
authors.forename: Eduardo Antônio Gonçalves
authors.initials: EA
authors.lastname: Ramos
authors.forename: Fernando Martins
authors.initials: FM
authors.lastname: Carvalho
authors.forename: Virgínia Maria Castro Ribeiro
authors.initials: VM
authors.lastname: Guedes
authors.forename: Lídia Silva
authors.initials: LS
authors.lastname: Souza
authors.forename: Cristiane Machado
authors.initials: CM
authors.lastname: Rocha
authors.forename: Andréa Barreto
authors.initials: AB
authors.lastname: Soares
authors.forename: Luciana de Freitas
authors.initials: Lde F
authors.lastname: Velloso
authors.forename: Izolete Santos
authors.initials: IS
authors.lastname: Macedo
authors.forename: Fernanda Edna Araujo
authors.initials: FE
authors.lastname: Moura
authors.forename: Marilda
authors.initials: M
authors.lastname: Siqueira
authors.forename: Silvana
authors.initials: S
authors.lastname: Fortes
authors.forename: Cibele Cruz
authors.initials: CC
authors.lastname: de Jesus
authors.forename: Christiane Maria Gaspar
authors.initials: CM
authors.lastname: Santiago
authors.forename: Ana Maria da Silva
authors.initials: AM
authors.lastname: Carvalho
authors.forename: Eurico
authors.initials: E
authors.lastname: Arruda
mesh_terms: D000208:Acute Disease; D001826:Body Fluids / Q000821:virology; D001938:Brazil / Q000453:epidemiology; D015331:Cohort Studies; D003631:Day Care, Medical; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015994:Incidence; D010850:Picornaviridae Infections / Q000453:epidemiology; D011446:Prospective Studies; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000821:virology; D012229:Rhinovirus; D012621:Seasons; D014505:Urban Population; D014777:Virus Diseases / Q000453:epidemiology*