title: Identification of new low-molecular-weight glutenin subunit genes in wheat.
abstract: To clarify the composition of low-molecular-weight glutenin subunits (LMW-GSs) in a soft wheat cultivar, we cloned and characterized LMW-GS genes from a cDNA library and genomic DNA in Norin 61. Based on alignment of the conserved N- and C- terminal domains of the deduced amino-acid sequences, these genes are classified into 12 groups. One of these groups (group 5), the corresponding gene of which has not been reported previously, contains two additional hydrophobic amino-acid clusters interrupting the N-terminal repetitive domain. Other groups (groups 11 and 12), which were not identified in other cultivars as a protein product, showed all eight cysteines in the C-terminal conserved domain. With specific primer sets for these groups it was revealed that Glu-D3 and Glu-A3 encoded the former and the latter, respectively. Both groups of genes were expressed in immature seeds. The presence of these groups of LMW-GSs may affect the dough strength of soft wheat.
authors.affiliation: National Agricultural Research Center for Western Region, 6-12-1 Nishifukatsu, Fukuyama, Hiroshima 721-8514, Japan. tmikeda@affrc.go.jp
authors.forename: T. M.
authors.initials: TM
authors.lastname: Ikeda
authors.forename: T.
authors.initials: T
authors.lastname: Nagamine
authors.forename: H.
authors.initials: H
authors.lastname: Fukuoka
authors.forename: H.
authors.initials: H
authors.lastname: Yano