Determination of 8-hydroxy-2'-deoxyguanosine in untreated urine by capillary electrophoresis with UV detection.
A capillary electrophoresis method with UV detection was developed for the determination of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in untreated urine samples. The calibration graph for 8-OHdG in urine is linear in the concentration range 10-500 mg/l. and the detection limit is 5 mg/l (17 microM). 8-OHdG was determined in urine from oncological patients treated by radiation therapy. Its concentrations relative to creatinine were found to be in the range 10-47 microg 8-OHdG/l mg creatinine (4-19 micromol 8-OHdG/mmol creatinine). The overall time of the analysis of a urine sample was less than 15 min.
Kvasnicová
Vladimira
V
3rd Faculty of Medicine, Centre of Biomedical Sciences, Charles University, Ruská 87, 100 00 Prague 10, Czech Republic.
Samcová
Eva
E
Jursová
Alice
A
Jelínek
Ivan
I
D000080242:8-Hydroxy-2'-Deoxyguanosine; D002138:Calibration; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000652:urine; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine