title: Performance of immunoassays in screening for opiates, cannabinoids and amphetamines in post-mortem blood.
abstract: Several immunoassay methods for screening of abused drugs in whole blood were evaluated in post-mortem forensic toxicology. Blood samples known to be positive or negative for opiates, cannabinoids or amphetamines by gas chromatography-mass spectrometry (GC-MS) were analysed by EMIT II Plus and EMIT d.a.u., Syva RapidTest and Triage 8 after acetone precipitation. In these experiments, the EMIT immunoassay method was modified by using the Dade Behring VIVA analyser to detect substances more sensitively. Low concentrations of abused drugs were detected in blood samples. The sensitivities of the modified EMIT method for opiates, cannabinoids and amphetamines were 100, 86 and 98%, respectively, whereas the values were below 86% with the other methods. The specificities of all immunoassay methods for opiates and cannabinoids were 83% or above but 51-85% for amphetamines. Sample rejection occurred in a few cases with the EMIT amphetamine assays. The modified EMIT immunoassay system presented here seems to be useful for screening of drugs of abuse in post-mortem blood samples, especially when urine is not available.
authors.affiliation: Department of Forensic Medicine, University of Helsinki, PO Box 40, FIN-00014, Helsinki, Finland.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Hino
authors.forename: Ilkka
authors.initials: I
authors.lastname: Ojanperä
authors.forename: Ilpo
authors.initials: I
authors.lastname: Rasanen
authors.forename: Erkki
authors.initials: E
authors.lastname: Vuori
mesh_terms: D000661:Amphetamine / Q000097:blood*; D002186:Cannabinoids / Q000097:blood*; D017248:Enzyme Multiplied Immunoassay Technique; D005189:False Positive Reactions; D005554:Forensic Medicine / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D009294:Narcotics / Q000097:blood*; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000295:instrumentation* / Q000379:methods
chemical_list: D002186:Cannabinoids; D009294:Narcotics; D000661:Amphetamine