[Osteocalcin and type I collagen mRNA expression of rat marrow stromal cells under induction condition].
OBJECTIVE
To investigate osteocalcin and type I collagen mRNA expression of rat marrow stromal cells under induction condition.


METHODS
Cultured rat marrow stromal cells were induced by osteogenic compound(composed of dexamethasone 10(-3) mol/L, beta glyceralphosphate 10 mmol/L, ascorbic acid 50 micrograms/ml). Osteocalcin and type I collagen mRNA expression were measured using RT-PCR technique.


RESULTS
It was found that rat marrow stromal cells in induced group have an increased time-dependent expression of osteocalcin and type I collagen mRNA, in comparison with control groups. And the necessity of 1,25-(OH)2D3 in induced osteocalcin mRNA expression was confirmed.


CONCLUSIONS
The cultured rat marrow stromal cells kept the undifferentiated state and had osteogenic potential testified by increased osteocalcin and type I collagen mRNA expression when induced.
Chen
Huaiqing
H
Institute of Biomedical Engineering, West China Medical Center, Sichuan University, Chengdu 610041, China.
Han
Jun
J
Li
Liang
L
Lu
Jianghua
J
Zhang
Wensheng
W
Chen
Rui
R
D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D015675:Osteocalcin / Q000096:biosynthesis* / Q000235:genetics; D010012:Osteogenesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017422:Sequence Analysis, DNA; D013250:Steroid Hydroxylases / Q000494:pharmacology; D017154:Stromal Cells / Q000378:metabolism*
D024042:Collagen Type I; D012333:RNA, Messenger; D015675:Osteocalcin; D013250:Steroid Hydroxylases; C406322:vitamin D 1-alpha hydroxylase