[Inhibitory effects of genistein on the synthesis of DNA and the protein expression of cyclin D1 in human gastric carcinoma cell-line].
In order to study the inhibitory effects of genistein on the growth of human gastric carcinoma cells and to explore its possible mechanism, cell proliferation and DNA incorporation tests are applied. Immunocytochemistry is also applied in this study to detect the protein expression of cyclin D1 after the cell-line is exposed to genistein. The results showed that the growth of the cells and the synthesis of DNA is decreased in a marked dose-dependent manner after the treatment with genistein. Genistein also decreased the protein expression of cyclin D1. The above mentioned results indicate that genistein inhibits the growth of gastric carcinoma cells and the possible mechanism of this inhibition might be resulted from inhibiting the synthesis of DNA and the protein expression of cyclin D1.
Song
Danfeng
D
Public Health School, Harbin Medical University, Harbin 150001, China.
Liu
Ying
Y
Wang
Xinjian
X
Yang
Yanmei
Y
D000230:Adenocarcinoma / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D019938:Cyclin D1 / Q000096:biosynthesis* / Q000235:genetics; D004273:DNA, Neoplasm / Q000096:biosynthesis* / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D019938:Cyclin D1; D019833:Genistein