[Modulatory effect of orphanin FQ on the IL-1beta transcripts in hippocampus].
Using in situ hybridization and immuno-fluorescent double labeling method, we investigated the function of orphanin FQ on interlenkin-lbeta (IL-lbeta) transcripts in hippocampus. Intracerebroventricularly (icv) administrated IL-lbeta antibody reduced IL-l and TNF-alpha secreted from peritoneal macrophage. The high level of IL-lbeta transcript in hippocampus elicited by traumatic stress was blocked by icv injection of orphanin FQ (0.55 nmol), which was reversed by orphanin FQ receptor antagonist ([phe(1)Psi(CH(2)-NH)Gly(2)] nociceptin-(1-l3)-NH(2)). Opioid receptor-like receptor transcripts were found in neuron, astrocyte and microglia. Based on the results, we conclude that orphanin FQ functions as a neuroimmune modulator, and provokes immune response via mediation of IL-lbeta derived from neuron, astrocyte and microglia in hippocampus.
Zhao
H
H
State Key Laboratory of Medical Neurobiology, Medical Center of Fudan University, Department of Neurobiology, Shanghai 200032.
Du
L N
LN
Jiang
J W
JW
Wu
G C
GC
Cao
X D
XD
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D007276:Injections, Intraventricular; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D017737:Macrophages, Peritoneal / Q000378:metabolism; D008297:Male; D017628:Microglia / Q000378:metabolism; D018847:Opioid Peptides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011957:Receptors, Opioid; D000097629:Nociceptin
D000276:Adjuvants, Immunologic; D007375:Interleukin-1; D018847:Opioid Peptides; D011957:Receptors, Opioid; D000097629:Nociceptin