title: In vitro culture of Glugea sp.
abstract: Recently the low host specificity of some microsporidians has been demonstrated and it has been indicated that many of these micro-organisms could be transmitted from invertebrates to mammals and adapt to changes in temperature. In this work, we demonstrate the first successful in vitro culture of a fish microsporidia of the genus Glugea on larval cells of the mosquito Aedes albopictus at 28 degrees C, and we show ultrastructural aspects of the different life cycle stages. It was impossible on salmon cells CHSE-214 at 21 degrees C. This study will be valuable for further work in biochemistry and immunology in addition to chemotherapy for microsporidiosis humans and animals.
authors.affiliation: Vircell, S.L. Poligonolindustrial Camino del Jau, Santa Fe, Granada, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Lores
authors.forename: M J
authors.initials: MJ
authors.lastname: Rosales
authors.forename: C
authors.initials: C
authors.lastname: Mascaró
authors.forename: A
authors.initials: A
authors.lastname: Osuna
mesh_terms: D000330:Aedes / Q000166:cytology / Q000469:parasitology*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D005399:Fishes / Q000469:parasitology*; D007814:Larva / Q000166:cytology / Q000469:parasitology; D008018:Life Cycle Stages / Q000502:physiology; D016814:Microsporidia / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure; D012474:Salmon / Q000469:parasitology