[Effect of ligustrazine peritoneal dialysate on CD40 expression of rat peritoneal mesothellial cells].
OBJECTIVE
To explore the effect of Ligustrazine (LGZ) affecting the expression of CD40 on mesothelial cells (MC).


METHODS
Rat's peritoneal MC isolated from peritoneal cavity were cultured and maintained under the defined conditions in vitro. They were cultured with 4.25% peritoneal dialysis solution (PDS), 4.25% PDS added LGZ (40 mg/L), 4.25% PDS added IFN-gamma (100 u/ml) and 4.25% PDS added IFN-gamma and LGZ for 30 min, the DMEM/F12 cultural medium was taken as control. The expression of CD40 on MC was detected by reverse transcript polymerase chain reaction (RT-PCR) and flow cytometry analysis.


RESULTS
MC cultured in vitro expressed CD40 markedly, the expression of CD40 mRNA and its protein was markedly up-regulated following stimulation with 4.25% PDS or IFN-gamma and 4.25% PDS. LGZ (40 mg/L) could reduce the expression of CD40 mRNA and its protein significantly as it was added in 4.25% PDS and 4.25% PDS with IFN-gamma.


CONCLUSIONS
The PDS stimulates the expression of CD40 on MC. LGZ has the function of down-regulating the expression of CD40 on MC. During long-term continuous ambulatory peritoneal dialysis, it might be helpful to alleviate chronic inflammatory reaction by adding LGZ into standard PDS, therefore prevent or retard the occurence and development of peritoneal fibrosis.
Yang
X
X
First Affiliated Hospital of Sun Yat-sen University of Medical Sciences, Guangzhou 510080.
Ye
R G
RG
Yang
Q Q
QQ
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D019013:CD40 Antigens / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D015314:Dialysis Solutions / Q000494:pharmacology; D004848:Epithelium / Q000378:metabolism*; D005355:Fibrosis / Q000517:prevention & control; D008297:Male; D010530:Peritoneal Dialysis / Q000009:adverse effects; D010537:Peritoneum / Q000166:cytology*; D011719:Pyrazines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats
D000894:Anti-Inflammatory Agents, Non-Steroidal; D019013:CD40 Antigens; D015314:Dialysis Solutions; D011719:Pyrazines; D012333:RNA, Messenger; C017953:tetramethylpyrazine