Electrochromatography with a 2.7 mm inner diameter monolithic column.
Monolithic columns of 2.7 mm I.D. have been prepared and used in electrochromatography (EC) separation. Although capillary electrochromatography (CEC) has higher separation efficiency, it displays some shortcomings, such as limited sample loadability and restricted concentration detectability etc. In this paper, we investigate the feasibility of EC separation with millimeter diameter monolithic columns. By using a designed preparation method of monolithic column packed with about 150 microm quartz sand, the effect of Joule heating can be reduced, and the processes of frit making and column packing can be avoided. The concentration detectability of the EC is improved comparing with that of CEC. Moreover, the separation efficiency of 52,000 plates/m was achieved with a 70 mm length and 2.7 mm I.D. monolithic column.
Qu
Qi-shu
QS
Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, PR China.
He
You-zhao
YZ
Gan
Wu-er
WE
Deng
Ning
N
Lin
Xiang-qin
XQ
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation*; D008855:Microscopy, Electron, Scanning