title: The little difference: in vivo analysis of pheromone discrimination in Schizophyllum commune.
abstract: The B mating type of Schizophyllum commune is defined by a multi-specific pheromone/receptor system. The interaction of pheromone receptors and their ligands, encoded by the Balpha locus, triggers sexual development. The receptors belong to the family of G protein-coupled seven-transmembrane-domain receptors, while the ligands are small lipopeptide pheromones. A productive interaction is only possible between molecules derived from different specificities. There is no induction of sexual development by pheromones of self-specificity. Since there are nine versions of different specificity for pheromones and receptors in Balpha, this system can be used to study multi-ligand discrimination. We investigated pheromone discrimination using chimeric receptor molecules and the influence of single point mutations on activation profiles of the receptor.
authors.affiliation: Friedrich-Schiller-University, Institute of Microbiology, Winzerlaer Strasse 10, 07745, Jena, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Gola
authors.forename: Erika
authors.initials: E
authors.lastname: Kothe
mesh_terms: D001483:Base Sequence; D002628:Chemoreceptor Cells / Q000737:chemistry / Q000502:physiology*; D003433:Crosses, Genetic; D017931:DNA Primers; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D016296:Mutagenesis; D010641:Phenotype; D010675:Pheromones / Q000502:physiology*; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012567:Schizophyllum / Q000235:genetics / Q000502:physiology*
chemical_list: D017931:DNA Primers; D010675:Pheromones; D011993:Recombinant Fusion Proteins