title: Role of alcohol and genetic polymorphisms of CYP2E1 and ALDH2 in breast cancer development.
abstract: OBJECTIVE
  We examined the potential association between alcohol consumption and genetic polymorphisms in the alcohol metabolizing enzymes, CYP2E1 and ALDH2, in individual susceptibility to breast cancer in a Korean study population.
  
  
  METHODS
  Three hundred and forty-six histologically confirmed breast cancer patients and 377 controls with no present or previous history of cancer were recruited from several teaching hospitals in Seoul during 1995-2001. The CYP2E1 RsaI polymorphism was determined by a real time PCR method, and the ALDH2 Glu487 Lys polymorphism was determined by a PCR method with confronting two-pair primers (PCR-CTPP).
  
  
  RESULTS
  The drinking women had a 1.4-fold risk for breast cancer (95% CI = 0.99-2.11) compared to never drinkers after adjustment for age and family history of breast cancer. No statistically significant overall differences were seen in the genotype frequencies between breast cancer cases and controls. However, the "ever"-drinking women with the CYP2E1 c2 allele containing genotypes had a 1.9-fold risk (95% CI = 0.99-3.83) for developing breast cancer compared to non-drinkers with the CYP2E1 c1/c1 genotype (P for interaction = 0.043).
  
  
  CONCLUSIONS
  This study therefore suggests that the CYP2E1 c2 allele may influence the individual susceptibility to breast cancer in alcohol-consuming women.
authors.affiliation: Department of Preventive Medicine, General Surgery and Cancer Research Institute, Seoul National University College of Medicine, 27 Yongon-Dong, Chongno-Gu, Seoul 110-799, Korea.
authors.forename: Ji-Yeob
authors.initials: JY
authors.lastname: Choi
authors.forename: Josef
authors.initials: J
authors.lastname: Abel
authors.forename: Thomas
authors.initials: T
authors.lastname: Neuhaus
authors.forename: Yon
authors.initials: Y
authors.lastname: Ko
authors.forename: Volker
authors.initials: V
authors.lastname: Harth
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hamajima
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tajima
authors.forename: Keun-Young
authors.initials: KY
authors.lastname: Yoo
authors.forename: Sue Kyung
authors.initials: SK
authors.lastname: Park
authors.forename: Dong-Young
authors.initials: DY
authors.lastname: Noh
authors.forename: Wonshik
authors.initials: W
authors.lastname: Han
authors.forename: Kuk-Jin
authors.initials: KJ
authors.lastname: Choe
authors.forename: Sei-Hyun
authors.initials: SH
authors.lastname: Ahn
authors.forename: Sook-Un
authors.initials: SU
authors.lastname: Kim
authors.forename: Ari
authors.initials: A
authors.lastname: Hirvonen
authors.forename: Daehee
authors.initials: D
authors.lastname: Kang
mesh_terms: D000328:Adult; D000428:Alcohol Drinking / Q000235:genetics* / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D000483:Alleles; D001943:Breast Neoplasms / Q000201:enzymology / Q000235:genetics*; D016022:Case-Control Studies; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D018450:Disease Progression; D000431:Ethanol / Q000378:metabolism; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015183:Restriction Mapping; D012307:Risk Factors
chemical_list: D017931:DNA Primers; D000431:Ethanol; D019392:Cytochrome P-450 CYP2E1; C096127:ALDH2 protein, human; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial