Lipid peroxidation is not increased in patients with untreated mild-to-moderate hypertension.
In contrast with the huge amount of experimental data available, only few and somewhat unconvincing clinical studies support the hypothesis that oxidative stress is involved in the early stages of essential hypertension in humans. Isoprostanes are chemically stable lipid peroxidation products of arachidonic acid, the quantification of which provides a novel approach to the assessment of oxidative stress in vivo. The main objective of this study was to quantify the urinary levels of 15-F(2t)-IsoP in the early stages of essential hypertension, using gas chromatography/mass spectrometry, by comparing 30 patients with never-treated mild-to-moderate hypertension with 30 gender- and age-paired healthy controls. Urinary 15-F(2t)-IsoP levels were not significantly different in hypertensive patients (69+/-36 pmol/mmol creatinine) compared with controls (75+/-34 pmol/mmol creatinine, 95% confidence intervals on differences: -23 to 13). No significant correlation was found between basal urinary 15-F(2t)-IsoP levels and age, low-density lipoprotein cholesterol, glucose, clinical pulse pressure, carotid intima-media thickness, left ventricular mass index, or aortic pulse wave velocity. In conclusion, this study shows that lipid peroxidation is not increased in never-treated mild-to-moderate hypertension. This suggests that oxidative stress is not implicated in the pathogenesis of human essential hypertension, at least in the early stages.
Cracowski
Jean-Luc
JL
Pharmacology Department, Grenoble University Hospital, Grenoble, France. Jean-Luc.Cracowski@ujf-grenoble.fr
Baguet
Jean-Philippe
JP
Ormezzano
Olivier
O
Bessard
Janine
J
Stanke-Labesque
Françoise
F
Bessard
Germain
G
Mallion
Jean-Michel
JM
D001794:Blood Pressure / Q000502:physiology; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D015237:Dinoprost / Q000031:analogs & derivatives*; D028441:F2-Isoprostanes / Q000652:urine; D005260:Female; D006801:Humans; D006973:Hypertension / Q000378:metabolism* / Q000503:physiopathology; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D016037:Single-Blind Method
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D028441:F2-Isoprostanes; C075750:8-epi-prostaglandin F2alpha; D002784:Cholesterol; D015237:Dinoprost