Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit.
We have shown that dermatophyte species can be easily identified on the basis of a DNA sequence encoding a part of the large-subunit (LSU) rRNA (28S rRNA) by using the MicroSeq D2 LSU rRNA Fungal Sequencing Kit. Two taxa causing distinct dermatophytoses were clearly distinguished among isolates of the Trichophyton mentagrophytes species complex.
Ninet
Béatrice
B
Division des Maladies Infectieuses, Hôpital Cantonal Universitaire, 1211 Geneva.
Jan
Isabelle
I
Bontems
Olympia
O
Léchenne
Barbara
B
Jousson
Olivier
O
Panizzon
Renato
R
Lew
Daniel
D
Monod
Michel
M
D000818:Animals; D003883:Arthrodermataceae / Q000302:isolation & purification*; D001483:Base Sequence; D002415:Cats; D004271:DNA, Fungal / Q000032:analysis; D004285:Dogs; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012339:RNA, Ribosomal, 28S / Q000032:analysis* / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D012689:Sequence Homology, Nucleic Acid
D004271:DNA, Fungal; D012339:RNA, Ribosomal, 28S; D011933:Reagent Kits, Diagnostic