[Study on low density lipoprotein receptor of adult Schistosoma japonicum].
OBJECTIVE
To provide a theoretical basis for the study of vaccines against Schistosoma japonicum, the receptor for human LDL in the tegument of adult Schistosoma japonicum was investigated.


METHODS
Proteins existed in adult Schistosoma japonicum membrane were extracted by Triton X-100 and purified through reverse-phase high performance liquid chromatography (HPLC), and the main protein peaks were then collected separately. 125I-LDL of human plasma as the ligand, through the radioautography and radioligand binding assay, the protein which can bind human serum 125I-LDL specifically was identified. The molecular weight and IEF were detected by SDS-PAGE.


RESULTS
According to the radioautography and radioligand binding assay, a protein with retention time of 10.5 min was proved to be able to bind human serum 125I-LDL specifically. SDS-PAGE revealed that the molecular weight of the purified protein is 60-65 kDa, and its IEF is 6.7.


CONCLUSIONS
LDL binding protein may exist on the surface of both male and female adult Schistosoma japonicum with the function of obtaining cholesterol from host circulating system.
Wang
Liang
L
Department of Parasitology, Sun Yat-sen University of Medical Sciences, Guangzhou 510089.
Li
Zhuo-ya
ZY
Wang
Yi
Y
He
Ai
A
Xie
Min-hui
MH
Zhan
Xi-mei
XM
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D011817:Rabbits; D011869:Radioligand Assay; D011973:Receptors, LDL / Q000032:analysis* / Q000302:isolation & purification; D012549:Schistosoma japonicum / Q000737:chemistry*
D008077:Lipoproteins, LDL; D011973:Receptors, LDL