title: The expression of adhesion molecules in muscle biopsies: the LFA-1/VLA-4 ratio in polymyositis.
abstract: OBJECTIVE
  The expression of three pairs of adhesion receptors and ligands was examined in 22 consecutive muscle biopsies showing morphological signs of inflammation.
  
  
  METHODS
  The following groups were studied: patients with polymyositis (PM) (n=7), patients with myositis that did not fulfil criteria for PM, i.e. suspected PM (n=5), patients with other diseases, with no clinical signs of inflammatory myopathy (n=6), and a small group of non-PM inflammatory myopathies (n=4). The endothelial expression of ICAM-1, VCAM-1 and E-selectin was evaluated, as was the cellular expression of LFA-1, VLA-4 and SLex. In addition, the expression of MHC class I and II was studied.
  
  
  RESULTS
  The ratio between the number of cells expressing LFA-1 and VLA-4 showed significant differences between the groups, with the lowest values in PM.
  
  
  CONCLUSIONS
  The LFA-1/VLA-4 ratio should be suitable for diagnostic purposes. Our findings also indicate that the VLA-4/VCAM-1 system is important for chronic T cell inflammation in muscle, in line with findings in other "hidden" organs like joints and the central nervous system.
authors.affiliation: Neuromuscular Unit, Department of Clinical Neurosciences and Locomotion, University Hospital, Linköping, Sweden. bjorn.lindvall@lio.se
authors.forename: B
authors.initials: B
authors.lastname: Lindvall
authors.forename: K
authors.initials: K
authors.lastname: Dahlbom
authors.forename: K G
authors.initials: KG
authors.lastname: Henriksson
authors.forename: U
authors.initials: U
authors.lastname: Srinivas
authors.forename: J
authors.initials: J
authors.lastname: Ernerudh
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D002196:Capillaries / Q000378:metabolism / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002452:Cell Count; D019040:E-Selectin / Q000096:biosynthesis; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D039041:Integrin alpha4beta1 / Q000096:biosynthesis*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D007962:Leukocytes / Q000378:metabolism / Q000473:pathology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D009844:Oligosaccharides / Q000096:biosynthesis; D017285:Polymyositis / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D011237:Predictive Value of Tests; D000080506:Sialyl Lewis X Antigen; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis
chemical_list: D015815:Cell Adhesion Molecules; D019040:E-Selectin; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D039041:Integrin alpha4beta1; D016169:Lymphocyte Function-Associated Antigen-1; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1