title: [The Role of Calcium Ion in Apotopsis of HL-60 Cells Induced by VP-16]
abstract: To study the significance of calcium in the apoptosis of HL-60 cells induced by VP-16, the technology of flow cytometry, confocal laser scanning microscopy and Western blot were used. The results showed that VP-16 could induced the apoptosis of HL-60 cells and transient increase of intracellular calcium concentration; EGTA [ethylene glycol-bis(2-aminoethyl)-N,N,N',N'-tetraacetic acid], that could combine the extracellular calcium, did not prevent the apoptosis of HL-60 cells. BAPTA-AM [1,2-bis(2-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid tetrakis (acetoxy-methyl) ester], however, a chelating agent of intracellular calcium ions, could prevent apoptosis and the release of cytochrome C from HL-60 cells. It was concluded that the calcium plays an important role in apoptosis and the release of cytochrome C.
authors.affiliation: Institute of Hematology, Beijing Medical University, Beijing 100044, China; Medicine and Pharmacology Analysis Centre, Peking University, Beijing 100084, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Shi
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Lan
authors.initials: L
authors.lastname: Yuan
authors.forename: Qihua
authors.initials: Q
authors.lastname: He
authors.forename: Shenwu
authors.initials: S
authors.lastname: Wang
authors.forename: Debing
authors.initials: D
authors.lastname: Wang