[Establishment of A + G ligation mediated polymerase chain reaction and its application in in vivo footprinting study].
OBJECTIVE
To establish a new ligation mediated PCR method for the work in in vivo footprinting study of some regions of low G-residue content or some regions of G-residue free.


METHODS
The A > G chemical cleavage method of Maxam-Gilbert sequencing was modified for ligation mediated PCR after A + G chemical cleavage and separate the PCR products by sequencing PAGE.


RESULTS
A new A + G ligation mediated method was established and could be used in vivo footprinting study.


CONCLUSIONS
Our results indicated that the newly developed method could analyze the DNA-Protein interaction at both A-residue and G-residue, enabled us to get more information and enlarged the scope of its application.
Ji
X
X
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, PUMC and CAMS, Beijing 100005.
Liu
D
D
Xu
D
D
Li
L
L
Wang
J
J
D000818:Animals; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology; D007942:Leukemia, Experimental / Q000473:pathology; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D023302:Protein Footprinting / Q000379:methods*; D014407:Tumor Cells, Cultured