Cloning and characterization of the genes coding for antigen 85A, 85B and 85C of Mycobacterium avium subsp. paratuberculosis.
Three genes encoding the secreted proteins (antigen 85-A, B, and C) of Mycobacterium avium subsp. paratuberculosis were cloned, sequenced and studied. The complete sequences of these three 85-complex proteins revealed their similarity with 85-complex proteins of other mycobacterial species. Specifically, these sequences showed 99% homology with M. avium 85-complex protein sequences. The multiple homology analysis of these sequences revealed that variations occur at only certain amino acid positions and this is true with all other 85-complex protein sequences of mycobacteria. However, the proposed three conserved regions involved in fibronectin binding in other mycobacteria were observed in N-terminal regions 85A, B and C of M. avium subsp. paratuberculosis.
Dheenadhayalan
Veerabadran
V
Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Shin
Kwang-Soon
KS
Chang
Chao-Fu
CF
Chang
Chin-Dong
CD
Wang
Shih-Jon
SJ
McDonough
Sean
S
McDonough
Patrick
P
Stehman
Susan
S
Shin
Sang
S
Torres
Alfonso
A
Chang
Yung-Fu
YF
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009162:Mycobacterium avium / Q000235:genetics*; D016415:Sequence Alignment; D017385:Sequence Homology
D000942:Antigens, Bacterial