title: [High glucose impaires glucose transport and PKB activity in adipocytes of rats].
abstract: OBJECTIVE
  To explore the effect of high glucose on glucose transport activity, protein kinase B (PKB) activity and glucose transporter 4 (GLUT4) in primary cultured rat adipocytes.
  
  
  METHODS
  Isolated rat adipocytes were cultured for 24 h at different glucose concentrations (5, 10, 15 and 25 mmol.L-1). The glucose uptake, cellular and membrane GLUT4 expression, PKB protein expression, and PKB serine phosphorylation and activity were measured.
  
  
  RESULTS
  These adipocytes treated with glucose of different concentrations showed that high glucose impaired glucose uptake, PKB phosphorylation and activity, and up-regulated GLUT4 translocation, but didn't affect protein expression of PKB and GLUT4.
  
  
  CONCLUSIONS
  High glucose can induce insulin resistance; the mechanism may be involved in the effect of high glucose on PKB serine phosphorylation and activity as well as GLUT4 function.
authors.affiliation: Department of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Hui-xia
authors.initials: HX
authors.lastname: Liu
authors.forename: Bi-xiu
authors.initials: BX
authors.lastname: He
authors.forename: Bi-lian
authors.initials: BL
authors.lastname: Chen
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology*; D007333:Insulin Resistance; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009004:Monosaccharide Transport Proteins; D011518:Proto-Oncogene Proteins; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt; D005947:Glucose