Assessment of minimal residual disease in childhood non-hodgkin lymphoma by polymerase chain reaction using patient-specific primers.
A feasibility study was undertaken to identify patient-specific primers (PSPs) from childhood non-Hodgkin lymphoma (NHL) specimens to detect minimal residual disease (MRD). Eleven tumor specimens were amplified using immunoglobulin heavy chain and T-cell receptor primers to identify PSPs, which were then used to evaluate staging/follow-up specimens. Disease was detected in 19 of 21 staging and 16 of 17 follow-up specimens. Among seven patients in remission by 1 month, PSPs identified MRD in follow-up specimens. This study demonstrated the feasibility of PSPs to identify disease in staging and follow-up specimens, which could be used to develop strategies for MRD analysis in a larger setting.
Sabesan
Vani
V
Indiana University School of Medicine, Indianapolis, USA.
Cairo
Mitchell S
MS
Lones
Mark A
MA
Perkins
Sherrie L
SL
Morris
Erin
E
Sposto
Richard
R
Van De Ven
Carmella
C
Shiramizu
Bruce
B
D000293:Adolescent; D002051:Burkitt Lymphoma / Q000175:diagnosis / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000032:analysis*; D005240:Feasibility Studies; D005260:Female; D019676:Genes, T-Cell Receptor delta; D019675:Genes, T-Cell Receptor gamma; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007223:Infant; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis* / Q000235:genetics*; D016399:Lymphoma, T-Cell / Q000175:diagnosis / Q000235:genetics; D008297:Male; D018365:Neoplasm, Residual / Q000175:diagnosis / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D004273:DNA, Neoplasm; D007143:Immunoglobulin Heavy Chains