[Preliminary examination of a LB-H2O2 substance that inhibit Neisseria gonorrhea growth].
OBJECTIVE
To study the possible mechanism of H2O2-producing lactobacilli inhibited neisseria gonorrhoeae growth.


METHODS
H2O2-producing lactobacilli were coincubated with neisseria gonorrhoeae in vitro, LB+ cell were disrupted by sonication, the protein in the lactobacilli lysate was separated by filtration through different MW sizes membranes and was examined for the ability to inhibit N. gonorrhoeae catalase activity.


RESULTS
When coincubation medium was at pH 5.0, there was a significant decrease in the gonococcal catalase activity, the lysates of LB+ also effectively inhibited gonococcal catalase activity. This inhibition was retained upon heating of lysate to 100 degrees C for 15 minutes but was lost with proteinase K treatment.


CONCLUSIONS
The LB+ may inhibit growth of gonococci by production of catalase inhibitors.
Zheng
H
H
Department of Dermatology, PUMC Hospital, CAMS and PUMC, Beijing 100730.
Cao
J
J
Jin
H
H
Wang
J
J
D002374:Catalase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism* / Q000494:pharmacology; D007778:Lactobacillus / Q000302:isolation & purification / Q000378:metabolism*; D009344:Neisseria gonorrhoeae / Q000201:enzymology*; D014621:Vagina / Q000382:microbiology
D006861:Hydrogen Peroxide; D002374:Catalase