title: Variations in flow, duration, and concentration do not change the final lung concentration of melphalan after isolated lung perfusion in rats.
abstract: OBJECTIVE
  The influences of flow, perfusion time, and concentration on melphalan (MN) lung levels in a rat model of isolated lung perfusion (ILuP) were studied.
  
  
  METHODS
  ILuP was performed in WAG/Rij rats by using a single-pass system with 0.5, 0.05, 0.01, and 0.005 mg of MN. Subsequently ILuP with 0.05 mg MN was performed during 30 min with a flow rate of 0.5 ml/min, during 60 min with a flow rate of 0.5 ml/min, and during 30 min with a flow rate of 1.0 ml/min.
  
  
  RESULTS
  The lung MN levels of 0.05 mg were significantly lower as compared with 0.5 mg (p = 0.02). In the second experiment, no significant differences were seen in lung levels of MN between the different groups.
  
  
  CONCLUSIONS
  The final lung MN levels were only determined by the absolute amount administered.
authors.affiliation: Department of Thoracic and Vascular Surgery, University of Antwerp, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Romijn
authors.forename: J M H
authors.initials: JM
authors.lastname: Hendriks
authors.forename: B P
authors.initials: BP
authors.lastname: Van Putte
authors.forename: G
authors.initials: G
authors.lastname: Guetens
authors.forename: E A
authors.initials: EA
authors.lastname: De Bruijn
authors.forename: P E Y
authors.initials: PE
authors.lastname: Van Schil
mesh_terms: D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008168:Lung / Q000378:metabolism*; D008297:Male; D008558:Melphalan / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D009994:Osmolar Concentration; D010477:Perfusion; D011652:Pulmonary Circulation; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D018906:Antineoplastic Agents, Alkylating; D008558:Melphalan