[Role of TNF-alpha and ICAM-1 in pathogenesis of cerebral malaria].
OBJECTIVE
To investigate the role of TNF-alpha and ICAM-1 in the pathogenesis of cerebral malaria.


METHODS
Immunohistochemical method and ELISA were employed to examine the expression of ICAM-1 on the brain microvessel endothelium and to detect the production of serum TNF-alpha in P. berghei ANKA strain infected-CBA/J mice.


RESULTS
Serum TNF-alpha levels of mice were apparently higher and the ICAM-1 expression was more evident in P. berghei ANKA infected-CBA/J mice than in control groups. rTNF-alpha i.p. injection could enhance the development of CM and the expression of ICAM-1 on brain endothelial cells(EC).


CONCLUSIONS
Excessive production of TNF-alpha may mediate the expression of ICAM-1 on brain EC and hence cause the development of CM.
Che
L M
LM
Department of Parasitology, Capital University of Medical Sciences, Beijing 100054.
Niu
Y X
YX
Li
H Z
HZ
Chen
X N
XN
D000818:Animals; D001921:Brain / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D016779:Malaria, Cerebral / Q000209:etiology* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010962:Plasmodium berghei; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage / Q000502:physiology*
D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1