[Killing effect of schistosomula of Schistosoma japonicum by tissues/organs of Microtus fortis in vitro].
OBJECTIVE
To screen the specific tissues/organs of Microtus fortis (MF) that have the activity of anti-schistosomula of Schistosoma japonicum.


METHODS
The homogenate supernatant of the heart, liver, lung, spleen, kidney, muscle and fresh serum of MF and those of mice were mixed with schistosomula respectively and cultured at 37 degrees C with 5% CO2. The schistosomula-killing effect of Schistosoma japonicum was observed.


RESULTS
There was no significant difference between the tissues/organs of MF and those of the mice in schistosomula-killing effect (P > 0.05), as it was the same with the tissues/organs of MF (P > 0.05) and between the fresh serum of MF and its complement-inactivated serum (P > 0.05). The schistosomula-killing effect of the serum of MF was stronger than that of the mice (P = 0.001), and the same compared to the tissues/organs of MF (P < 0.05). Between the 40% fresh serum of MF and its 20% fresh serum, there was a significant difference (P < 0.001).


CONCLUSIONS
The schistosomula-killing effect of the serum of MF was stronger than that of mice, and it was the same with the tissues/organs of MF.
Shen
Qun-xi
QX
Molecular Biology Research Center, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Hu
Wei-xin
WX
Xu
Bing
B
D000818:Animals; D003411:Arvicolinae; D046508:Culture Techniques; D006321:Heart / Q000254:growth & development; D007668:Kidney / Q000254:growth & development; D007814:Larva / Q000187:drug effects; D008099:Liver / Q000254:growth & development; D009206:Myocardium; D011817:Rabbits; D012549:Schistosoma japonicum / Q000187:drug effects*; D012556:Schistosomicides / Q000494:pharmacology*; D014020:Tissue Extracts / Q000494:pharmacology*
D012556:Schistosomicides; D014020:Tissue Extracts