Decrease in malate dehydrogenase activities in peripheral leucocytes of type 1 diabetic dogs.
The activities of the enzymes involved in the malate-aspartate shuttle were measured in peripheral leucocytes of dogs with type 1 diabetes mellitus. In the diabetic dogs, fasting plasma glucose concentrations were twofold greater than control levels despite insulin injections and the activities of malate dehydrogenase (MDH), which plays a crucial role in the malate-aspartate shuttle, were decreased remarkably. The cytosolic ratio of MDH/lactate dehydrogenase (LDH) activity (M/L ratio) in leucocytes of the diabetic dogs was significantly lower than that of normal control dogs. The decrease of the M/L ratio appeared to reflect depression of energy metabolism in leucocytes of the diabetic dogs. The M/L ratio may be a useful parameter to evaluate metabolic conditions in diabetic dogs.
Arai
T
T
Department of Veterinary Science, Nippon Veterinary and Animal Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan. tarai@nvau.ac.jp
Nakamura
M
M
Magori
E
E
Fukuda
H
H
Sako
T
T
D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology* / Q000662:veterinary*; D004283:Dog Diseases / Q000201:enzymology*; D004285:Dogs; D005260:Female; D007962:Leukocytes / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008297:Male
D008291:Malate Dehydrogenase