Overload-induced androgen receptor expression in the aged rat hindlimb receiving nandrolone decanoate.
This study's purpose was to examine whether functional overload with nandrolone decanoate (ND) administration increased muscle mass and steroid receptor concentration in aged rat soleus (Sol) and plantaris (Plan) muscle. ND (6 mg/kg body wt) was administered once a week for 4 wk, whereas control rats received sesame seed oil injections. Functional overload of the hindlimb Sol and Plan was induced by synergistic gastrocnemius muscle ablation at the beginning of the fourth week. Adult (5 mo of age) and aged rats (25 mo of age) were randomly assigned to four groups: control, overload, control-ND, and overload-ND. Seven days of functional overload increased adult Sol muscle mass 27%, whereas the aged Sol muscle mass did not change. The aged overloaded Sol muscle receiving ND significantly increased muscle weight by 35% and total muscle protein by 24%. Aged Plan muscle did not increase muscle weight with overload or ND treatment. Androgen receptor protein was induced by ND treatment and functional Ov, and combining the two treatments induced Sol androgen receptor protein concentration above either alone. Sol glucocorticoid receptor protein concentration increased in overload groups of both ages. ND administration can increase aged Sol muscle mass and protein content after 7 days of functional overload, and the cooperative induction of androgen receptor may be important for this response.
Lee
Won Jun
WJ
Integrative Muscle Biology Laboratory, Exercise Science Department, University of South Carolina, Columbia, South Carolina 29208, USA.
McClung
Joseph
J
Hand
G A
GA
Carson
James A
JA
D045930:Anabolic Agents / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D004247:DNA / Q000096:biosynthesis; D006614:Hindlimb / Q000502:physiology*; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D009277:Nandrolone / Q000494:pharmacology*; D009929:Organ Size / Q000187:drug effects / Q000502:physiology; D005082:Physical Exertion / Q000502:physiology*; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D011914:Rats, Inbred BN; D011916:Rats, Inbred F344; D011944:Receptors, Androgen / Q000096:biosynthesis*; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis
D045930:Anabolic Agents; D009124:Muscle Proteins; D011944:Receptors, Androgen; D011965:Receptors, Glucocorticoid; D012313:RNA; D009277:Nandrolone; D004247:DNA