title: [Studies on chemical structure of polysaccharide from fruit body of Coriolus versicolor].
abstract: OBJECTIVE
  To study the structure of polysaccharides from Coriolus versicolor.
  
  
  METHODS
  By chromatography a homogeneous polysaccharide B-1-3 was obtained. The structure of B-1-3 was elucidated using the method of total acid hydrolysis, methylation, and 1D and 2D NMR.
  
  
  RESULTS
  B-1-3 was found to be mainly composed of Glc by total acid hydrolysis. Its molecular weight was estimated to be 3.16 x 10(5) by HPLC method. Methylation analysis and 1H and 13CNMR indicated the main chain of B-1-3 consisted of beta-D-1,4-Glc and beta-D-1,3-Glc, and branch chains were situated, beta-D-1,3,6-Glc and beta-D-1,4,6-Glc. 1H and 13CNMR also showed that beta-D-Glc-1-->3-beta-D-Glc-beta-D-Glc-1-->4-beta-D-Glc and beta-D-Glc-1-->4-beta-D-Glc-beta-D-Glc-1-->4-beta-D-Glc fragments existed in B-1-3.
  
  
  CONCLUSIONS
  B-1-3 is a glucan which main chain was composed of beta-D-1,4-Glc and beta-D-1,3-Glc beta-D-1,6-Glc.
authors.affiliation: Edible Fungi Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China. zhangjs888@etang.com
authors.forename: J S
authors.initials: JS
authors.lastname: Zhang
authors.forename: W W
authors.initials: WW
authors.lastname: Han
authors.forename: Y J
authors.initials: YJ
authors.lastname: Pan
mesh_terms: D005936:Glucans / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D011124:Polyporaceae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D005936:Glucans; D011134:Polysaccharides