[Isoelectric focusing analysis of Echinococcus multilocularis protoscolex antigens].
OBJECTIVE
To understand the components of the crude antigens of Echinococcus multilocularis protoscoleces.


METHODS
Isoelectric focusing was carried out to fractionate the protein components of E. multilocularis protoscoleces. The individual fractions were collected and subjected to SDS-PAGE, the gels were analyzed by a gel analysis system.


RESULTS
The pI values of individual fractions varied from 4.0 to 8.5, while the molecule weights of main protein bands were 72/73, 62/63, 42/44, 35/36, 34 and 21/22 kDa.


CONCLUSIONS
An alternative separation method of crude antigens of Echinococcus multilocularis was established.
Li
S P
SP
Institute of Infectious and Parasitic Disease, Chongqing University of Medical Science, Chongqing 400016.
Chen
Y T
YT
Jiang
C P
CP
Qiu
J M
JM
Yu
D G
DG
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D004446:Echinococcus / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing
D000947:Antigens, Helminth