title: [Capillary zone electrophoresis for determination of thymulin in human plasma].
abstract: OBJECTIVE
  To establish a capillary zone electrophoresis (CZE) method for determination of thymulin in human blood plasma. Thymulin is a nonapeptide produced by the thymic epithelium.
  
  
  METHODS
  The uncoated capillary used was 75 microns ID x 57 cm (50 cm effective length). The experimental conditions were as follows: the running buffer was phosphate-borate buffer containing 50 mmol.L-1 Na2B4O7-10 mmol.L-1 Na2HPO4 (pH 9.10); running voltage was 12 kV; operated temperature was 25 degrees C; running time was 13 minutes, detective wavelength was 200 nm.
  
  
  RESULTS
  The linear range of the thymulin standard curve was 1-10 micrograms.mL-1, gamma = 0.9990, the average recovery was 70.97% with RSD of 3.32%, the RSD of reproducibility was 3.05%.
  
  
  CONCLUSIONS
  The method of CZE for determination of thymulin in human plasma is simple and reliable.
authors.affiliation: Insitute of Nutrition and Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Ning
authors.initials: N
authors.lastname: Qu
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Xia
mesh_terms: D002675:Child, Preschool; D019075:Electrophoresis, Capillary; D006801:Humans; D010949:Plasma / Q000737:chemistry*; D013935:Thymic Factor, Circulating / Q000032:analysis*
chemical_list: D013935:Thymic Factor, Circulating