Inhibition effect of cyclosporin A on human retinal pigment epithelial cells in vitro.
OBJECTIVE
To select effective drugs against cellular proliferation in the vitreous.


METHODS
Cyclosporin A (0.125 mg/l-4.0 mg/l) was added to cultures of human retinal pigment epithelial (RPE) cells with or without macrophage-conditioned medium (MCM). Proliferation rate of the cells was measured with (3H)-thymidine incorporation and liquid scintillation techniques on days 3 and 5.


RESULTS
Cyclosporin A at a dosages of 0.125 mg/l had a slight inhibition on human RPE cells proliferation (-3.8%-4.1%, P > 0.05). Cyclosporin A at a dose ranging from 0.25 mg/l to 4.0 mg/l inhibited cellular proliferation effectively and in a dose-dependent manner (8.7%-95.1%, P < 0.05 r = 0.94, P < 0.001) with its ID50 of 1.49 mg/l. In the culture of RPE with MCM, the inhibition on day 5 was more effective than that on day 3.


CONCLUSIONS
Cyclosporin A had an effective inhibition on human RPE cell proliferation and it may be of potential use clinically.
Wang
Z
Z
Eye Center, Xijing Hospital, 4th Military Medical University, Xian 710032, China.
Hui
Y
Y
Hu
M
M
D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D003864:Depression, Chemical; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology; D010857:Pigment Epithelium of Eye / Q000166:cytology*
D007166:Immunosuppressive Agents; D016572:Cyclosporine