Synthesis of labelled PNA oligomers by a post-synthetic modification approach.
The preparation of t-butoxycarbonyl (Boc)-protected O(4)-(o-nitrophenyl) thymine peptide nucleic acid (PNA) monomer is described. This PNA monomer was incorporated into PNA oligomer sequences. The post-synthetic modification of the oligomers to yield fluorescently-labelled PNA oligomers was studied before and after the removal of the protecting groups. In both cases, the desired fluorescently-labelled PNA oligomer was obtained in good yields.
de la Torre
Beatriz G
BG
Institut de Biologia Molecular de Barcelona, C.S.I.C., Jordi Girona 18-26, E-08034 Barcelona, Spain.
Eritja
Ramon
R
D005456:Fluorescent Dyes / Q000138:chemical synthesis; D005563:Formic Acid Esters / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D009574:Nitro Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry
D005456:Fluorescent Dyes; D005563:Formic Acid Esters; D007202:Indicators and Reagents; D009574:Nitro Compounds; D009841:Oligonucleotides; D020135:Peptide Nucleic Acids