Daeganghwal-tang inhibits the stem cell factor-induced migration and inflammatory cytokines secretion in mast cells.
Traditional Oriental medicinal prescription, Daeganghwal-tang (DGHT) has been used for the treatment of rheumatoid arthritis (RA) in Korea. However, its effect in experimental models remains unknown. Recent reports suggest that in patients with RA, synovial mast cells increase in number and show signs of activation and inflammatory cytokines secretion. Our results show that stem cell factor (SCF) is a potent chemotactic factor for the mast cells in vitro. The chemotactic response to SCF was blocked by DGHT. When DGHT (1mg/ml) was added, the secretion of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta and IL-6 was inhibited by 60.1, 81.8, 72.5%, respectively in phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187-stimulated HMC-1 cells. In addition, the expression of TNF-alpha mRNA in HMC-1 cells was inhibited by DGHT (1mg/ml). These findings indicate that DGHT inhibits SCF-induced migration and PMA plus calcium ionophore-stimulated inflammatory cytokines secretion in mast cells.
Shin
H Y
HY
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan-city, Chonbuk 570-749, South Korea.
Jeong
H J
HJ
Na
H J
HJ
Hong
S H
SH
Lee
S K
SK
Lee
K N
KN
Song
Y S
YS
Kim
H M
HM
Shin
T Y
TY
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002464:Cell Migration Inhibition; D002633:Chemotaxis / Q000187:drug effects*; D016207:Cytokines / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D066298:In Vitro Techniques; D008233:Lymphotoxin-alpha / Q000378:metabolism; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D008518:Medicine, East Asian Traditional; D010529:Peritoneal Cavity / Q000166:cytology; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D019089:Stem Cell Factor / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D008233:Lymphotoxin-alpha; D010936:Plant Extracts; D012333:RNA, Messenger; D019089:Stem Cell Factor; D014409:Tumor Necrosis Factor-alpha; C473301:daeganghwal-tang