title: [Isolation and identification of steroidal saponins in total saponin from Dioscorea nipponica Makino].
abstract: OBJECTIVE
  To investigate the water-soluble steroidal saponins in total saponin from Dioscorea nipponica Makino and look for new active compounds.
  
  
  METHODS
  The compounds were isolated with silica gel, PTLC and HPLC, and their structures were elucidated by acid hydrolysis, physical and chemical data and spectral analysis (IR, NMR, MS, HMQC, HMBC) as well as chemical correlations.
  
  
  RESULTS
  The two steroidal saponins (water-insoluble saponin and water-soluble saponin) were isolated from the total saponin of Dioscorea nipponica Makino. The structures were elucidated as diosgenin 3-O-[alpha-L-rhamnopy ranosyl (1-->2)-[beta-D-glucopyranosyl(1-->3)]]-beta-D-glucopyranoside (I), diosgenin 3-O-[alpha-L-rhamnopyranosyl (1-->3)-alpha-L-rhamnopyranosyl (1-->4)-alpha-L-rhamnopyranosyl (1-->4)]-beta-D-glucopyranoside (II).
  
  
  CONCLUSIONS
  Compound II is a new steroidal saponin and firstly isolated from Dioscorea nipponica Makino. It was named as dioscin Dc.
authors.affiliation: Anhui Institute of Medical Sciences, Hefei 230061, China. dushuhu@ah163.com
authors.forename: Shu-hu
authors.initials: SH
authors.lastname: Du
authors.forename: Wen-ying
authors.initials: WY
authors.lastname: Liu
authors.forename: Tie-jun
authors.initials: TJ
authors.lastname: Fu
authors.forename: Bo-gang
authors.initials: BG
authors.lastname: Xia
authors.forename: Chong-dao
authors.initials: CD
authors.lastname: Xia
mesh_terms: D027742:Dioscorea / Q000737:chemistry*; D008968:Molecular Conformation; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D012503:Saponins; C479298:diosgenin 3-O-(rhamnopyranosyl-1-3-rhamnopyranosyl-1-4-rhamnopyranosyl-1-4)glucopyranoside; C479299:diosgenin 3-O-(rhamnopyranosyl-1-2-(glucopyranosyl-1-3))glucopyranoside