[Effect of naoyi'an on basic fibroblastic growth factor mRNA expression and tumor necrosis factor protein expression in brain of rats following intracerebral hemorrhage].
OBJECTIVE
To investigate the effect of Naoyi'an granule (NYAG) on basic fibroblastic growth factor (bFGF) mRNA expression and tumor necrosis factor (TNF) protein expression following intracerebral hemorrhage and provide the theoretical evidence of NYAG in treating intracerebral hemorrhage and promoting the rehabilitation of neural function.


METHODS
Model rats of intracerebral hemorrhage induced by infusion of collagenase VII into the caudate-putamen were used to determine the related parameters of behavior scores (BS), Northern blot, Western blot assay and optical density (OD) scanning in the model and the model treated with NYAG. And the data got from the two groups were compared.


RESULTS
BS in the model group began to lower 24 hrs after modeling and a significant decrease was shown 7 days later, while in the NYAG group, it decreased significantly three days after modeling, the difference between the two groups was significant (P < 0.05). Levels of bFGF mRNA expression and TNF protein expression increased after modeling, it reached the peak in three days and began to decrease gradually in seven days in both groups. However, the level in the NYAG group was higher than that in the model group in various times of the experimental process.


CONCLUSIONS
NYAG could enhance the bFGF expression and suppress the TNF expression so as to improve the behavior deficit in treating intracerebral hemorrhage, which may be one of the main mechanisms of NYAG for promoting the rehabilitation of neural function.
Zhang
Hua-biao
HB
Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Zhongnan University, Changsha 410078.
Li
Xing-qun
XQ
Zhang
Ming-xiang
MX
D000818:Animals; D001921:Brain / Q000378:metabolism; D002543:Cerebral Hemorrhage / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016222:Fibroblast Growth Factor 2