title: Cloning and characterization of the genes coding for antigen 85A, 85B and 85C of Mycobacterium avium subsp. paratuberculosis.
abstract: Three genes encoding the secreted proteins (antigen 85-A, B, and C) of Mycobacterium avium subsp. paratuberculosis were cloned, sequenced and studied. The complete sequences of these three 85-complex proteins revealed their similarity with 85-complex proteins of other mycobacterial species. Specifically, these sequences showed 99% homology with M. avium 85-complex protein sequences. The multiple homology analysis of these sequences revealed that variations occur at only certain amino acid positions and this is true with all other 85-complex protein sequences of mycobacteria. However, the proposed three conserved regions involved in fibronectin binding in other mycobacteria were observed in N-terminal regions 85A, B and C of M. avium subsp. paratuberculosis.
authors.affiliation: Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Veerabadran
authors.initials: V
authors.lastname: Dheenadhayalan
authors.forename: Kwang-Soon
authors.initials: KS
authors.lastname: Shin
authors.forename: Chao-Fu
authors.initials: CF
authors.lastname: Chang
authors.forename: Chin-Dong
authors.initials: CD
authors.lastname: Chang
authors.forename: Shih-Jon
authors.initials: SJ
authors.lastname: Wang
authors.forename: Sean
authors.initials: S
authors.lastname: McDonough
authors.forename: Patrick
authors.initials: P
authors.lastname: McDonough
authors.forename: Susan
authors.initials: S
authors.lastname: Stehman
authors.forename: Sang
authors.initials: S
authors.lastname: Shin
authors.forename: Alfonso
authors.initials: A
authors.lastname: Torres
authors.forename: Yung-Fu
authors.initials: YF
authors.lastname: Chang
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009162:Mycobacterium avium / Q000235:genetics*; D016415:Sequence Alignment; D017385:Sequence Homology
chemical_list: D000942:Antigens, Bacterial