Vacuolar system of ungerminated Colletotrichum graminicola conidia: convergence of autophagic and endocytic pathways.
Vacuoles of ungerminated Colletotrichum graminicola conidia engulf cytoplasmic structures by a process analogous to microautophagy, demonstrated by using a vacuolar membrane acid phosphatase marker. Fusion of vesicles with vacuoles, without deposition of the acid phosphatase reaction product has been observed, suggesting other pathways of material delivery to vacuoles than microautophagy. Plasma membrane invaginations, multivesicular bodies and retention of neutral red into small vesicles, which were internalized by the vacuole, were verified. These results provided evidence for endocytosis and an active endosomal system. Together, our findings with C. graminicola demonstrated that vacuoles are very dynamic compartments, playing roles in autophagy and endocytic processes.
Schadeck
Ruth Janice Guse
RJ
Department of Cellular Biology, Federal University of Paraná, C.P. 19031, 81531-990, PR, Curitiba, Brazil.
Randi
Marco Antonio Ferreira
MA
de Freitas Buchi
Dorly
D
Leite
Breno
B
D000135:Acid Phosphatase / Q000378:metabolism; D001343:Autophagy / Q000502:physiology*; D020231:Colletotrichum / Q000254:growth & development / Q000502:physiology* / Q000648:ultrastructure; D004705:Endocytosis / Q000502:physiology*; D009499:Neutral Red / Q000378:metabolism; D014617:Vacuoles / Q000502:physiology* / Q000648:ultrastructure
D009499:Neutral Red; D000135:Acid Phosphatase