Lipolytic and nutritive blood flow response to beta-adrenoceptor stimulation in situ in subcutaneous abdominal adipose tissue in obese men.
OBJECTIVE
beta-Adrenoceptor-mediated whole-body lipolysis is impaired in obesity. This study investigated whether local adipocyte beta-adrenergic sensitivity and changes in nutritive blood flow in subcutaneous abdominal adipose tissue contribute to this impaired response.


METHODS
Three microdialysis probes were placed in the subcutaneous abdominal adipose tissue of eight obese and nine lean men. Each probe was perfused with either 0.1, 1 and 10 microM isoprenaline; 1, 10 and 100 microM dobutamine or 1, 10 and 100 microM salbutamol, each dose for 45 min.


RESULTS
At baseline, interstitial glycerol concentrations and ethanol out/in ratios were comparable between groups. During nonselective beta-, beta(1)- and beta(2)-adrenergic stimulation, interstitial glycerol concentrations increased and ethanol out/in ratios decreased similarly in obese and lean men.


CONCLUSIONS
The lipolytic and nutritive blood flow response to beta(1)- beta(2)- and nonselective beta-adrenergic stimulation in situ is comparable in lean and obese male subjects. The present data suggest that a blunted beta-adrenergic sensitivity of the fat cell and an impaired local nutritive blood flow response do not contribute to the previously reported diminished whole-body beta-adrenoceptor-mediated lipolytic response in obese males.
Schiffelers
S L H
SL
Department of Human Biology, Maastricht University, The Netherlands.
Akkermans
J A P
JA
Saris
W H M
WH
Blaak
E E
EE
D034861:Abdominal Wall / Q000098:blood supply / Q000502:physiology*; D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000098:blood supply / Q000378:metabolism*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000420:Albuterol / Q000494:pharmacology; D004280:Dobutamine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D017551:Microdialysis; D008875:Middle Aged; D009765:Obesity / Q000503:physiopathology*
D000318:Adrenergic beta-Agonists; D000431:Ethanol; D004280:Dobutamine; D007545:Isoproterenol; D005990:Glycerol; D000420:Albuterol