title: Kir2 potassium channels in rat striatum are strategically localized to control basal ganglia function.
abstract: Parkinson's disease is the most frequent movement disorder caused by loss of dopaminergic neurons in the midbrain. Intentions to avoid side effects of the conventional therapy should aim to identify additional targets for potential pharmacological intervention. In principle, every step of a signal transduction cascade such as presynaptic transmitter release, type and occupation of postsynaptic receptors, G protein-mediated effector mechanisms, and the alterations of pre- or postsynaptic potentials as determined by the local ion channel composition, have to be considered. Due to their diversity and their widespread but distinct localizations, potassium channels represent interesting candidates for new therapeutic strategies. As a first step, the present report aimed to study in the striatum the cellular and subcellular distribution of the individual members of the Kir2 family, a group of proteins forming inwardly rectifying potassium channels. For this purpose polyclonal monospecific affinity-purified antibodies against the less conserved carboxyterminal sequences from the Kir2.1, Kir2.2, Kir2.3, and Kir2.4 proteins were prepared. All subunits of the Kir2 family were detected on somata and dendrites of most striatal neurons. However, the distribution of two of them was not homogeneous. Striatal patch areas were largely devoid of the Kir2.3 protein, and the Kir2.4 subunit was most prominently expressed on the tonically active, giant cholinergic interneurons of the striatum. These two structures are among the key players in regulating dopaminergic and cholinergic neurotransmission within the striatum, and therefore are of major importance for the output of the basal ganglia. The heterogeneous localization of the Kir2.3 and the Kir2.4 subunits with respect to these strategic structures pinpoints to these channel proteins as promising targets for future pharmacological efforts.
authors.affiliation: Institut für Anatomie, der Charité, Universitätsklinikum der Humboldt-Universität zu Berlin, Philippstrasse 12, D-10115 Berlin, Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Prüss
authors.forename: Mareike
authors.initials: M
authors.lastname: Wenzel
authors.forename: Dirk
authors.initials: D
authors.lastname: Eulitz
authors.forename: Achim
authors.initials: A
authors.lastname: Thomzig
authors.forename: Andreas
authors.initials: A
authors.lastname: Karschin
authors.forename: Rüdiger W
authors.initials: RW
authors.lastname: Veh
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D019556:COS Cells; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism* / Q000648:ultrastructure; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D007395:Interneurons / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D019581:Neuropil / Q000378:metabolism* / Q000648:ultrastructure; D010300:Parkinson Disease / Q000188:drug therapy / Q000378:metabolism* / Q000503:physiopathology; D015221:Potassium Channels / Q000378:metabolism; D024661:Potassium Channels, Inwardly Rectifying / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*
chemical_list: C490968:Kcnj4 protein, rat; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine