Molecular genetic diagnosis of pediatric cancer: current and emerging methods.
A wide array of diagnostic tests are available to evaluate molecular abnormalities in pediatric cancer. Classic cytogenetics, FISH, flow cytometry, PCR, and Southern blot analysis are in widespread use throughout pediatric hospitals. Examples of the application of these methods in pediatric cancer diagnosis are reviewed. Newer methods such as CGH, SKY, gene expression microarrays and proteomic methods are under active investigation andwill almost certainly lead to significant advances in our ability to diagnose and treat pediatric cancer.
Rowland
Jon M
JM
Department of Pathology, Children's Hospital and Research Center at Oakland, 747 52nd Street, Oakland, CA 94609, USA. jrowland@mail.cho.org
D015139:Blotting, Southern; D020732:Cytogenetic Analysis / Q000379:methods*; D005434:Flow Cytometry; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008967:Molecular Biology / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D010372:Pediatrics; D016133:Polymerase Chain Reaction