[The Application of RACE Technique to Clone the Full-Length cDNA of A Novel Leukemia Associated Gene LRP16]
LRP16 is a novel gene which was found in our laboratory by using methylation-sensitive restriction landmark genomic scanning (RLGS) technique. In order to clone the full-length cDNA of this leukemia relapse associated gene, the method of rapid amplification of cDNA end (RACE) was employed. By optimizing some procedures of RACE method, the 5'- and 3'-untranslated region of LRP16 cDNA was successfully sequenced. Then, the full length of LRP16 cDNA and open reading frame (ORF) was constructed and was registered in GenBank. The above-mentioned procedure demonstrated RACE technique is a rapid and sensitive method for cloning unknown gene. Especially, it is very useful to cloning the 5'- and 3'-untranslated region of a novel gene.
Han
Wei-Dong
WD
Department of Hematology, The Genral Hospital of PLA, Beijing 100853, China.
Yu
Li
L
Lou
Fang-Ding
FD
Wang
Quan-Shun
QS
Zhao
Yu
Y
Shi
Zi-Jiang
ZJ
Jin
Hai-Jie
HJ