The effects of triptolide on HLA antigens expression of corneal epithelial cells induced by interferon-gamma in vitro.
OBJECTIVE
To observe the effects of immunosuppressants triptolide (TL) and cyclosporine A (CSA) on HLA antigens expression induced by interferon-gamma(INF-gamma) in vitro.


METHODS
By using an indirect immunofluorescent method and analysing with ACAS-570, the abnormal HLA antigen expression of cultured corneal epithelial cells was induced by INF-gamma. After incubation with one of the immunosuppressants (CSA, TL) for 72 hrs, the amount of HLA-A BC and HLA-DR antigens was measured.


RESULTS
There was no significant difference (P > 0.05) between the group with CSA and the positive control group without CSA. In contrast to CSA, TL dramatically inhibited INF-gamma induced expression of HLA antigens of corneal epithelial cells (P < 0.001), compared with the control group without TL.


CONCLUSIONS
TL had direct inhibition on the expression of HLA-ABC and HLA-DR antigens induced by INF-gamma in vitro, while CSA had no obvious inhibition.
Zhao
Q
Q
Guangzhou Military General Hospital, Guangzhou 510010, China.
Liu
Y
Y
Li
Q
Q
D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology; D004224:Diterpenes / Q000494:pharmacology*; D019573:Epithelium, Corneal / Q000166:cytology / Q000276:immunology*; D004852:Epoxy Compounds; D006680:HLA Antigens / Q000096:biosynthesis* / Q000235:genetics; D006684:HLA-DR Antigens / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D010616:Phenanthrenes / Q000494:pharmacology*
D004224:Diterpenes; D004852:Epoxy Compounds; D006680:HLA Antigens; D006684:HLA-DR Antigens; D007166:Immunosuppressive Agents; D010616:Phenanthrenes; C001899:triptolide; D007371:Interferon-gamma; D016572:Cyclosporine