[Effect of artemether on nucleoside uptake and nucleic acid content in Schistosoma japonicum].
OBJECTIVE
To observe the effect of artemether (Art) on nucleoside uptake and nucleic acid content in Schistosoma japonicum.


METHODS
RNA and DNA contents of both male and female worms harbored in mice treated intragastrically (i.g.) with Art 300 mg/kg for 24 h or 48 h were determined, respectively. After in vivo drug treatment, the schistosomes recovered were in vitro maintained in drug-free medium containing [3H]adenosine, [5-(3)H] uridine or [methyl-3H]thymidine at a final concentration of 37 MBq/L or 74 MBq/L for 2 h or 4 h, the tritiated nucleoside uptake and incorporation into nucleic acid of schistosomes were measured.


RESULTS
The RNA and DNA contents of female worms recovered from the host 48 h after dosing were markedly decreased by 51.6% and 23.5%, respectively, while the RNA content of male worms showed 42.4% reduction. When the above-mentioned schistosomes were in vitro exposed to the tritiated nucleoside for 2 h or 4 h, apparent decrease in tritiated nucleoside uptake with reduction rates of 35.2%-50.1% was seen in female worms. The incorporation of [methyl-3H]thymidine into the female worm DNA 2 h after incubation was reduced by 71.4% while the incorporation of [3H]adenosine into the female worm RNA and DNA 4 h after incubation was reduced by 65.2% and 50.0%, respectively.


CONCLUSIONS
Art exhibited an apparent effect on the nucleic acid metabolism in schistosomes, especially in female worms.
Zhai
Z
Z
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Mei
J
J
You
J
J
Yao
M
M
Xiao
S
S
D000818:Animals; D000077549:Artemether; D037621:Artemisinins / Q000494:pharmacology*; D018509:DNA, Helminth / Q000378:metabolism*; D005260:Female; D008297:Male; D051379:Mice; D009705:Nucleosides / Q000378:metabolism; D018510:RNA, Helminth / Q000378:metabolism*; D012549:Schistosoma japonicum / Q000187:drug effects / Q000378:metabolism*; D012556:Schistosomicides / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000494:pharmacology*
D037621:Artemisinins; D018509:DNA, Helminth; D009705:Nucleosides; D018510:RNA, Helminth; D012556:Schistosomicides; D012717:Sesquiterpenes; D000077549:Artemether