Expression of cytosolic and plastid acetyl-coenzyme A carboxylase genes in young wheat plants.
Expression of cytosolic and plastid acetyl-coenzyme A carboxylase (ACCase) gene families at the mRNA level was analyzed in developing wheat (Triticum aestivum) plants. The major plastid ACCase mRNA level is high in the middle part of the plant and low in roots and leaf blades. An alternative plastid ACCase transcript initiated at a different promoter and using an alternative 5' splice site for the first intron accumulates to its highest level in roots. Cytosolic ACCase mRNA also consists of two species, one of which is present at approximately a constant level, whereas the other accumulates to a high level in the lower sheath section. It is likely that different promoters are also responsible for the two forms of cytosolic ACCase mRNA. The abundances of cytosolic and plastid ACCase mRNAs in the sheath section of the plant are similar. ACCase protein level is significantly lower in the leaf blades, in parallel with changes in the total ACCase mRNA level. Homoeologous ACCase genes show the same expression patterns and similar mRNA levels, suggesting that none of the genes was silenced or acquired new tissue specificity after polyploidization.
Podkowinski
Jan
J
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637.
Jelenska
Joanna
J
Sirikhachornkit
Anchalee
A
Zuther
Ellen
E
Haselkorn
Robert
R
Gornicki
Piotr
P
D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism*; D015152:Blotting, Northern; D003600:Cytosol / Q000201:enzymology; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005810:Multigene Family; D010735:Phosphoglycerate Kinase / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D018087:Plastids / Q000201:enzymology*; D011123:Polyploidy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014908:Triticum / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D012333:RNA, Messenger; D010735:Phosphoglycerate Kinase; D000103:Acetyl-CoA Carboxylase