[Determination of resibufogenin in xinli pills by RP-HPLC].
A method has been developed for the quantitative determination of Resibufogenin in Xinli pills by RP-HPLC. The stainless steel column(200 x 4.6 mm) was packed with ODS-3 (particle size 5 microns). The mobile phase was a mixture of methanol-water (60:40). The flow rate was 1 ml/min. The column temperature was 30 degrees C. The detection wavelength was 299 nm. The retention time for recibufogenin was 5.6 min. The average recovery for resibufogenin was 99.9%. The linear calibration curve for resibufogenin was obtained in the range 53.0-159.0 micrograms with the correlation coefficient 0.9998.
Zhang
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Pharmaceutical Factory, Guangdong Institute of Drug Research, Guangzhou 510440.
Xu
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D000664:Amphibian Venoms / Q000737:chemistry*; D000818:Animals; D002018:Bufanolides / Q000032:analysis*; D002025:Bufonidae; D002316:Cardiotonic Agents / Q000737:chemistry*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D008419:Materia Medica / Q000737:chemistry*; D010946:Plants, Medicinal
D000664:Amphibian Venoms; D002018:Bufanolides; D002316:Cardiotonic Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D008419:Materia Medica; C005734:bufogenin