Some biochemical properties of melatonin and the characterization of a relevant metabolite arising from its interaction with H2O2.
Melatonin is an efficient protector against hydrogen peroxide(H2O2)-induced lipid peroxidation and acts in a concentration-dependent manner. Hydrogen peroxide is rather a water stable molecule which is able to cross the cell membrane much better than some important free radicals such as superoxide anion, and consequently its local production can lead to significant spread by diffusion. In this paper we report data regarding some biochemical properties of melatonin as well as the chemical characterization of the major product formed from the interaction between melatonin and H2O2 (N1-acetyl-N2-formyl-5-methoxykynuramine) that are consistent with previous data reported by other authors. The effect of melatonin on catalase, glutathione peroxidase and superoxide dismutase in in vitro and in vivo experiments is also reported.
Carampin
Paolo
P
Department of Pharmacological Sciences, University of Padova, Padova, Italy.
Rosan
Stefania
S
Dalzoppo
Daniele
D
Zagotto
Giuseppe
G
Zatta
Paolo
P
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002855:Chromatography, Thin Layer; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D015227:Lipid Peroxidation; D013058:Mass Spectrometry; D008550:Melatonin / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D013055:Spectrophotometry, Infrared; D013482:Superoxide Dismutase / Q000378:metabolism
D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D008550:Melatonin