title: [Improvement in the preparation of Paragonimus westermani chromosome for karyotype analysis].
abstract: OBJECTIVE
  To improve Paragonimus westermani chromosome preparation technique.
  
  
  METHODS
  After being exposed to colchicine, the gonadal cells of P. westermani were treated by the following procedures: hypotonicity, fixation, dropping onto a slide and staining.
  
  
  RESULTS
  The chromosome number of P. westermani is 22, and the karyotype is 2m + 6Sm + 14t chromosome.
  
  
  CONCLUSIONS
  The improved technique of chromosome preparation is feasible to operate and the chromosome is clear enough for karyotype analysis.
authors.affiliation: Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
authors.forename: Shao-hong
authors.initials: SH
authors.lastname: Chen
authors.forename: Zheng-shan
authors.initials: ZS
authors.lastname: Chang
authors.forename: Ming-gang
authors.initials: MG
authors.lastname: Chen
authors.forename: Yong-nian
authors.initials: YN
authors.lastname: Zhang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Feng
mesh_terms: D000818:Animals; D002871:Chromosome Banding / Q000379:methods*; D002875:Chromosomes / Q000648:ultrastructure*; D004285:Dogs / Q000469:parasitology; D007621:Karyotyping; D010238:Paragonimus / Q000235:genetics* / Q000302:isolation & purification