Different biochemical strategies of two neotropical fish to cope with the impairment of nitrogen excretion during air exposure.
The exposure of fish to air is normally expected to interfere with the nitrogen excretion process. Hoplias malabaricus and Hoplerythrinus unitaeniatus, two teleost species, display distinct behaviors in response to decreases in natural reservoir water levels, although they may employ similar biochemical strategies. To investigate this point, plasma levels of ammonia, urea, uric acid, and the two urea cycle enzymes, ornithine carbamoyl transferase (OCT) and arginase (ARG), as well as glutamine synthetase (GS) were determined for both species after exposure to air. Plasma ammonia increased gradually during exposure to air, but only H. malabaricus showed increased concentrations of urea. Plasma uric acid remained very low in both fish. Enzymatic activities (mean +/- SD, micromol min(-1) g protein(-1)) of H. malabaricus showed significant increases (P<0.05, N = 6) in OCT from 0.84 +/- 0.05 to 1.42 +/- 0.03, in ARG from 8.07 +/- 0.47 to 9.97 +/- 0.53 and in GS from 1.15 +/- 0.03 to 2.39 +/- 0.04. The OCT and ARG enzymes remained constant in H. unitaeniatus (N = 6), but GS increased from 1.49 +/- 0.02 to 2.06 +/- 0.03. Although these species are very closely related and share the same environment, their biochemical strategies in response to exposure to air or to increased plasma ammonia are different.
Polez
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VL
Departamentos de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brasil.
Moraes
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Santos Neto
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D000388:Air; D000641:Ammonia / Q000097:blood; D000818:Animals; D001119:Arginase / Q000097:blood; D015415:Biomarkers / Q000097:blood; D005399:Fishes / Q000378:metabolism*; D005974:Glutamate-Ammonia Ligase / Q000097:blood; D009584:Nitrogen / Q000378:metabolism*; D009954:Ornithine Carbamoyltransferase / Q000097:blood; D013045:Species Specificity; D014508:Urea / Q000097:blood; D014527:Uric Acid / Q000097:blood
D015415:Biomarkers; D014527:Uric Acid; D000641:Ammonia; D014508:Urea; D009954:Ornithine Carbamoyltransferase; D001119:Arginase; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen