Sequence analysis of the MSP 1 gene of Plasmodium falciparum isolates from Hainan, China.
OBJECTIVE
To obtain the complete sequence and analyze the diversity of the MSP 1 molecule from the Chinese isolates of Plasmodium falciparum.


METHODS
Genomic DNA was prepared directly from blood samples spotted on filter papers from 2 malaria patients from Baoting County, Hainan Province. PCR amplification of the target gene was carried out using 5 pairs of oligonucleotides specific for the MSP 1 gene. Direct sequencing of the target gene fragments was performed using ABI PRISM Dye Terminator Cycle Sequencing Ready Reaction Kit (Perkin Elmer) in a automatic ABI PRISM 310 Genetic Analyzer.


RESULTS
For the first time, two complete sequences of the MSP1 gene from two Chinese isolates of Plasmodium falciparum were obtained. A comparison with the previously reported sequences identified them as members of the MAD20 allelic family. The deduced amino acid sequences of the MSP1 from this two Chinese isolates were identical with each other except for Blocks 2, 4 and 8.


CONCLUSIONS
The sequences of the MSP 1 from two Chinese isolates of P. falciparum belong to the MAD20 allelic family. Minor variations through the whole sequences exist compared with the MAD20 sequence. The results provide the first evidence of the diversity of the MSP 1 molecule from Chinese isolates of Plasmodium falciparum.
Jiang
G
G
Department of Medical Parasitology, Guangdong College of Pharmacy, Guangzhou 510224.
Liu
R
R
Daubenberger
C A
CA
Pluschke
G
G
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D006801:Humans; D020066:Merozoite Surface Protein 1 / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017421:Sequence Analysis
D000953:Antigens, Protozoan; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins