[Detection of circulating antigen in urine from mice infected with Toxoplasma tachyzoites by SPA-ELISA].
OBJECTIVE
To explore a simple and convenient immunoassay for early diagnosis of Taxoplasma infection.


METHODS
Urine samples collected from three groups of mice infected with different doses of tachyzoites were detected for Taxoplasma circulating antigen (TCA) by dot-ELISA using HRP-SPA as a second antibody (SPA-ELISA).


RESULTS
Taxoplasma circulating antigens were detected in all three groups of infected mice in contrast with the normal control group. Taxoplasma circulating antigen was detected on d6 and d3 after infection in mice of light- and moderate-infection groups, respectively.


CONCLUSIONS
SPA-ELISA is a simple and convenient method for early immunodiagnosis of recent Taxoplasma infection.
Jin
W G
WG
Department of Pediatric Medicine, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025.
Li
Y Z
YZ
Yu
S C
SC
Yang
H Z
HZ
D000818:Animals; D000953:Antigens, Protozoan / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007159:Immunologic Tests; D051379:Mice; D013205:Staphylococcal Protein A; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis* / Q000652:urine
D000953:Antigens, Protozoan; D013205:Staphylococcal Protein A