[Immunostimulatory activity elicited by CpG sequences in Cysticercus cellulosae paramyosin cDNA].
OBJECTIVE
To analyse the immunostimulatory activity of CpG sequences in cysticercus cellulosae paramyosin (also named Antigen B, AgB) cDNA.


METHODS
C57BL/6 mice were immunized with pcDNA3-AgB plasmid, pcDNA3-AgB' (CpG sequences were mutated), pcDNA3 or AgB protein and two weeks later, immune response was assayed by ELISA.


RESULTS
IgG and IgG2a were detectable at week 2 after immunization and continually increased until week 4. The antibody levels elicited by pcDNA3-AgB were significantly higher(P < 0.05) than those elicited by others.


CONCLUSIONS
After pcDNA3-AgB plasmid inoculation, the immune response of mouse was elicited not only by the AgB protein but also by the CpG immunostimulatory sequences in the AgB cDNA.
Sun
S H
SH
Department of Medical Genetic, Second Military Medical University, Shanghai 200433.
Guo
Y J
YJ
Wang
Q M
QM
Chen
R W
RW
D000818:Animals; D018899:CpG Islands / Q000276:immunology*; D003552:Cysticercus / Q000276:immunology*; D018076:DNA, Complementary / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids / Q000276:immunology; D014335:Tropomyosin / Q000235:genetics*; D019444:Vaccines, DNA / Q000276:immunology*
D018076:DNA, Complementary; D007074:Immunoglobulin G; D014335:Tropomyosin; D019444:Vaccines, DNA