YY1-DNA interaction results in a significant change of electronic context as measured by capacitance.
The detailed mechanism behind the processes of DNA-dependent RNA transcription initiation is largely unknown. When transcription initiation factors bind DNA, a significant change in the electrostatic state of the complex can result. Using electrical capacitance measurements of solutions of the YY1 zinc finger transcription initiation factor and the adeno-associated viral P5 promoter DNA, we observed a specific dielectric change when a protein-DNA complex was formed. We propose that complexation results in electrostatic changes that may trigger the markedly different electrical behavior, and offer a possible explanation for our results.
Choi
Chu H
CH
Harvard Medical School, Beth Israel Deaconess Medical Center, 99 Brookline Ave., Boston, MA 02215, USA.
Moser
Joel
J
Sabourin
Nicaulas A
NA
Blagoev
Krastan
K
Naughton
Michael
M
Usheva
Anny
A
Sobourin
Nicaulas
N
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D025802:Electric Capacitance; D004583:Electrons; D050981:Erythroid-Specific DNA-Binding Factors; D006801:Humans; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011994:Recombinant Proteins; D055672:Static Electricity; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism; D050986:YY1 Transcription Factor
D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D011994:Recombinant Proteins; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C494698:YY1 protein, human; D004247:DNA