Generation of functional inhibitory neurons in the adult rat hippocampus.
Several thousand new neurons are produced each day in the adult mammalian hippocampus, among which only excitatory granule cells (GCs) have thus far been identified. In the present study, we used mutant Semliki Forest Virus vectors to express enhanced green fluorescent protein in the hippocampus, and observed that approximately 14% of newly generated neurons in the dentate gyrus of adult rats are GABAergic basket cells (BCs). With the use of double whole-cell patch-clamp recordings from BC-GC pairs in hippocampal slices, we demonstrate that newly generated BCs in the dentate gyrus form inhibitory synapses with principal GCs. These data show for the first time that functional inhibitory neurons are recruited in the dentate gyrus of adult rats.
Liu
ShuHong
S
Neuroscience Research Group, Department of Physiology and Biophysics, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada, T2N 4N1.
Wang
Jian
J
Zhu
DongYa
D
Fu
YangPing
Y
Lukowiak
Ken
K
Lu
You Ming
YM
D000818:Animals; D001973:Bromodeoxyuridine; D002452:Cell Count; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D005260:Female; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000008:administration & dosage / Q000502:physiology; D005968:Glutamate Decarboxylase / Q000032:analysis / Q000096:biosynthesis; D049452:Green Fluorescent Proteins; D006624:Hippocampus / Q000166:cytology* / Q000254:growth & development / Q000821:virology; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis / Q000096:biosynthesis; D008164:Luminescent Proteins / Q000235:genetics; D008297:Male; D009433:Neural Inhibition / Q000502:physiology*; D009474:Neurons / Q000145:classification / Q000166:cytology* / Q000378:metabolism / Q000821:virology; D018408:Patch-Clamp Techniques; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D012672:Semliki forest virus / Q000235:genetics / Q000502:physiology; D009435:Synaptic Transmission / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D007527:Isoenzymes; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2; D001973:Bromodeoxyuridine