[The morphometric analysis of lung on protective effect of Chinese herbs 814 on elastase-induced emphysema in hamsters].
OBJECTIVE
To determine the protective effect of Chinese herbal medicine 814 on elastase-induced emphysema in hamsters.


METHODS
Animals were injected intratrachealy with elastase for emphysematous models, prophylactic-therapeutic groups were administrate with 814 through esophagus two weeks before the instillation of elastase untill animals were killed at three different time at first, second, and third month. Pulmonary artery pressure, blood gas analysis, heart index and the ratio of dried weight to wet weight of lungs (WW/DW) were examined. The lung paraffin sections were measured mean linear intercept (MLI), mean alveolar number (MAN), ratio of parenchyma area to total area (PA/TA) by the microscope-computer morphometric analysis system.


RESULTS
WW/DW in the prophylactic-therapeutic groups was recovered at the same level with the controls, whereas the emphysematous were significantly increased (P < 0.05); and compared with the emphysematous groups, the prophylactictherapeutic groups significantly decreased in MLI, increased in MAN and PA/TA (P < 0.001 or P < 0.05).


CONCLUSIONS
Administration of 814 could partly inhibit the development of emphysema induced by elestase in hamsters.
Song
R
R
Department of Pathology, Fuwai Cardiovascular Hospital, CAMS and PUMC, Beijing 100037.
Ruan
Y
Y
Liu
Y
Y
Han
X
X
D000818:Animals; D001784:Blood Gas Analysis; D006224:Cricetinae; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D004646:Emphysema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D008168:Lung / Q000473:pathology*; D008297:Male; D008647:Mesocricetus; D010196:Pancreatic Elastase; D008517:Phytotherapy
D004365:Drugs, Chinese Herbal; D010196:Pancreatic Elastase