[The establishment, freezing-state preservation and resuscitation of retinoblastoma SO-Rb70 cell line].
OBJECTIVE
To establish a new cell line of human retinoblastoma (Rb), frozen and resuscitated to provide a Rb cell reservoir for clinical and experimental research.


METHODS
The screening cells were washed and blown directly without centrifugation and then inoculated. Based on the cell-frozen principle, the Rb cell line was collected and established, then frozen in liquid nitrogen and resuscitated.


RESULTS
SO-Rb70 cell line was established successfully. 8 groups of cells were frozen. The resuscitation experiment was carried out for 6 times, 4 times being successful.


CONCLUSIONS
The preserved human Rb cells in liquid nitrogen grew normally after resuscitation with the maintenance of previous bioactivities and characteristics. This research helped to provide abundant material for further study of Rb.
Zheng
J
J
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Feng
G
G
Li
Y
Y
Lin
J
J
Yi
Y
Y
D002460:Cell Line; D015925:Cryopreservation; D006801:Humans; D007223:Infant; D008297:Male; D019572:Retinal Neoplasms / Q000473:pathology*; D012175:Retinoblastoma / Q000473:pathology*; D014407:Tumor Cells, Cultured