[Effect of ginsenosides in inducing proliferation and transcription factor of erythrocytic, granulo-monocytic and megakarocytic cell lines].
OBJECTIVE
To investigate the action of Ginsenosides (GS) in inducing transcription factor c-fos and GATA-1 to explore the mechanism of GS in hematopoietic cells.


METHODS
The proliferation effects of GS on granulocytic (HL-60), monocytic (U937), erythrocytic (K562) and megaryocytic (Meg-01) cell lines were observed by using proliferation test of MTT and colony formation of progenitor cells. The combining reaction of transcription factors c-fos and GATA-1 with nuclear protein antigen were analyzed by Western Blot after being treated by GS.


RESULTS
(1) GS (10 micrograms/ml) could stimulate and promote proliferation of 3 cell lines with significant difference between GS and non-GS control (P < 0.05 in all) in both MTT test and colony assay. (2) After treatment with GS, c-fos protein in HL-60, K562 and Meg-01 cell lines was increased by 1.5, 2.0 and 2.5 fold respectively, while U937 cell did not express c-fos. (3) Except that U937 cell hadn't expressed GATA-1, the other cell lines after the treatment by GS, the GATA-1 protein level was elevated to 1.5, 2.1 and 1.3 fold of that before treatment.


CONCLUSIONS
The proliferation of three lines initiated by GS was involved in transcription factor c-fos or GATA-1, which could pay the role in the GS induced up-regulation correlated with proliferation and differentiation of hematopoiesis.
Chen
X H
XH
Zhejiang Provincial Hospital of TCM, Hangzhou 310006.
Gao
R L
RL
Xu
W H
WH
D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins; D002455:Cell Division / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D050981:Erythroid-Specific DNA-Binding Factors; D050980:GATA Transcription Factors; D050982:GATA1 Transcription Factor; D036145:Ginsenosides / Q000302:isolation & purification / Q000494:pharmacology*; D018922:HL-60 Cells; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D020014:K562 Cells / Q000166:cytology; D005894:Panax / Q000737:chemistry; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism; D020298:U937 Cells / Q000166:cytology
D029742:Caenorhabditis elegans Proteins; D004268:DNA-Binding Proteins; D004365:Drugs, Chinese Herbal; C096869:ELT-1 protein, C elegans; D050981:Erythroid-Specific DNA-Binding Factors; D050980:GATA Transcription Factors; D050982:GATA1 Transcription Factor; C494688:GATA1 protein, human; D036145:Ginsenosides; D016760:Proto-Oncogene Proteins c-fos; D015534:Trans-Activators; D014157:Transcription Factors