title: Hemin binding, functional expression, and complementation analysis of Pap 31 from Bartonella henselae.
abstract: Growth of Bartonella henselae is strongly heme dependent, and B. henselae is unable to synthesize heme itself. At least five outer membrane-associated proteins from B. henselae bind hemin, including the 31-kDa protein designated Pap31. The gene of this protein was heterologously expressed in Escherichia coli M15(pREP4) and detected with monoclonal antibodies in the outer membrane fraction. Complementation of the hemA-deficient mutant E. coli K-12 EB53 (aroB tsx malT hemA) with pap31 demonstrated that this protein is involved in heme acquisition and may be an important virulence factor in the pathogenesis of B. henselae.
authors.affiliation: Institute of Medical Microbiology and Hygiene, University of Freiburg, Freiburg, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Zimmermann
authors.forename: Volkhard A J
authors.initials: VA
authors.lastname: Kempf
authors.forename: Emile
authors.initials: E
authors.lastname: Schiltz
authors.forename: Karin
authors.initials: K
authors.lastname: Oberle
authors.forename: Anna
authors.initials: A
authors.lastname: Sander
mesh_terms: D000911:Antibodies, Monoclonal / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D018416:Bartonella henselae / Q000235:genetics*; D001483:Base Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000235:genetics; D005816:Genetic Complementation Test; D006427:Hemin / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; C110491:Pap31 protein, Bartonella henselae phage 60457; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014764:Viral Proteins; D006427:Hemin