Physical mapping of the brain and ovarian aromatase genes in the Nile Tilapia, Oreochromis niloticus, by fluorescence in situ hybridization.
Cytochrome P450-aromatase enzyme (CYP19), which catalyses the conversion of androgens to oestrogens, is critical in ovarian differentiation and hence in the sex differentiation pathways of non-mammalian vertebrates. As in other fish species, distinct ovarian and brain aromatase genes have been identified in the Nile Tilapia, Oreochromis niloticus. Here we demonstrate by in situ hybridization that the two aromatase genes of this species are present on different chromosomes and that neither are located on the sex chromosomes. Hence, the aromatase genes are not the primary sex determination genes in O. niloticus.
Harvey
S C
SC
Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, Scotland. sch1@stir.ac.uk
Kwon
J Y
JY
Penman
D J
DJ
D000818:Animals; D001141:Aromatase / Q000235:genetics*; D001921:Brain / Q000378:metabolism; D002874:Chromosome Mapping; D017931:DNA Primers; D005260:Female; D017404:In Situ Hybridization, Fluorescence; D010053:Ovary / Q000378:metabolism; D019849:Sex Determination Processes; D017210:Tilapia / Q000235:genetics*
D017931:DNA Primers; D001141:Aromatase