[Application of dot immunogold filtration assay in antibody detection for cysticercosis].
OBJECTIVE
To detect serum antibody of cysticercosis by dot immunogold filtration assay for establishing a diagnostic kit for cysticercosis patients.


METHODS
Cyst fluid of cysticercus of Taenia solium after dialysis was used as diagnostic antigen in dot immunogold filtration assay and enzyme-linked immunosorbent assay to detect the serum antibody in patients with cysticercosis. Samples to be detected included 71 sera from patients with cysticercosis, 90 sera from healthy people, 20 sera from patients with other parasitic infections or brain tumor.


RESULTS
The sensitivity and specificity of dot immunogold filtration assay were 90.1%(64/71) and 95.6%(86/90), respectively. No positive reaction was recorded in cases with other diseases except one serum from a patient with brain tumor. The coincidence rate between dot immunogold filtration assay and enzyme-linked immunosorbent assay was 94.4% (152/161).


CONCLUSIONS
Dot immunogold filtration assay showed promising result for the diagnosis of cysticercosis.
Liu
Z P
ZP
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai, 200025.
Li
H
H
Zhu
L Y
LY
Liu
S
S
Xue
H C
HC
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D003551:Cysticercosis / Q000175:diagnosis*; D003552:Cysticercus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration; D006046:Gold; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D000909:Antibodies, Helminth; D011933:Reagent Kits, Diagnostic; D006046:Gold