title: Identification and isolation of foot-and-mouth disease virus from primary suspect cases in Korea in 2000.
abstract: The Republic of Korea had been free from foot and mouth disease (FMD) since 1934, until a recent outbreak in 2000. From March to April 2000, a total of 15 FMD outbreaks due to the serotype O virus were recorded. Coincidental outbreaks of FMD in cattle or pigs by the serotype O virus were reported in the region, including Taiwan, China, Japan, Russia and Mongolia. In this report, the results of emergency investigations of FMD cases on a dairy farm located approximately 5-km from the demilitarized zone in Korea are described. The causative agent of the disease was identified as the FMD virus O by reverse transcription-polymerase chain reaction (RT-PCR) assays using primers derived from the 3D polymerase, internal ribosome entry site (IRES), 1D/2B regions, enzyme-linked immunosorbent assay (ELISA) for antigen detection and typing. Sequence data of the partial 1D/2B region obtained from vesicular fluid showed close similarity (98% sequence identity) to the Kinmen isolate of the FMD virus O in Taiwan. The causative virus was isolated using black goat fetal lung cells following propagation in unweaned mice.
authors.affiliation: National Veterinary Research and Quarantine Service, Anyang, Gyeonggi, Republic of Korea.
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Shin
authors.forename: Hyun-Joo
authors.initials: HJ
authors.lastname: Sohn
authors.forename: Kang-Seuk
authors.initials: KS
authors.lastname: Choi
authors.forename: Byung-Jun
authors.initials: BJ
authors.lastname: Kwon
authors.forename: Cheong-Up
authors.initials: CU
authors.lastname: Choi
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Eui-Kyung
authors.initials: EK
authors.lastname: Hwang
authors.forename: Jong-Hyeon
authors.initials: JH
authors.lastname: Park
authors.forename: Jong-Yeom
authors.initials: JY
authors.lastname: Kim
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Choi
authors.forename: Ok-Kyung
authors.initials: OK
authors.lastname: Kim
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000276:immunology / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D004196:Disease Outbreaks; D005260:Female; D005536:Foot-and-Mouth Disease / Q000453:epidemiology* / Q000276:immunology / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007723:Korea / Q000453:epidemiology; D012463:Saliva / Q000821:virology
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral