title: [Determination on the glycosyl sequence of gypenoside A by TLC-FABMS].
abstract: The glycosyl sequence of gypenoside A (A) isolated from Gynostemma pentaphyllum Makino was determinated by TLC-FABMS. The gypenoside A was hydrolysed with cellulase and the hydrolysate showed eight spots on a TLC plate, and the FABMS analysis of these spots indicated they corresponded to gypenoside A, A-Rham, A-Glu, A-Rham-Glu, A-Rham-Glu, A-Glu-Glu, A-Rham-2Glu, A-Rham-2Glu in order. Two chains can be determined to be Rham-Glu, and Glu-Glu, respectively. So the glycosyl sequence of gypenoside A was identical of gypenoside XLIII.
authors.affiliation: Guangzhou Pharmaceutical Industrial Research Institute, Guangzhou 510240.
authors.forename: G
authors.initials: G
authors.lastname: Liu
authors.forename: D
authors.initials: D
authors.lastname: Guo
authors.forename: R
authors.initials: R
authors.lastname: Zhong
authors.forename: Z
authors.initials: Z
authors.lastname: Yao
authors.forename: H
authors.initials: H
authors.lastname: Wen
authors.forename: W
authors.initials: W
authors.lastname: He
authors.forename: W
authors.initials: W
authors.lastname: Hu
mesh_terms: D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D029774:Gynostemma / Q000737:chemistry*; D006868:Hydrolysis; D008968:Molecular Conformation; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000032:analysis* / Q000737:chemistry*
chemical_list: D012503:Saponins; C117083:gypentonoside A