An X-ray micro-tomography system optimised for the low-dose study of living organisms.
An X-ray micro-tomography system has been designed that is dedicated to the low-dose imaging of radiation sensitive living organisms and has been used to image the early development of the first few days of plant development immediately after germination. The system is based on third-generation X-ray micro-tomography system and consists of an X-ray tube, two-dimensional X-ray detector and a mechanical sample manipulation stage. The X-ray source is a 50kVp X-ray tube with a silver target with a filter to centre the X-ray spectrum on 22keV.A 100mm diameter X-ray image intensifier (XRII) is used to collect the two-dimensional projection images. The rotation tomography table incorporates a linear translation mechanism to eliminate ring artefact that is commonly associated with third-generation tomography systems. Developing maize seeds (Triticum aestivum) have been imaged using the system with a cubic voxel linear dimension of 100 microm, over a diameter of 25mm and the root lengths and volumes measured. The X-ray dose to the plants was also assessed and found to have no effect on the plant root development.
Jenneson
P M
PM
School of Electronics and Physical Sciences, University of Surrey, Guildford, UK. p.jenneson@surrrey.ac.uk
Gilboy
W B
WB
Morton
E J
EJ
Gregory
P J
PJ
D004867:Equipment Design; D008904:Miniaturization; D018517:Plant Roots / Q000254:growth & development*; D011829:Radiation Dosage; D012639:Seeds / Q000254:growth & development; D014057:Tomography, X-Ray Computed / Q000295:instrumentation*; D003313:Zea mays