[The protective effect against Toxoplasma infection in mice immunized with laser-irradiated Toxoplasma tachyzoites].
OBJECTIVE
To observe the level of immune responses and protective immunity in mice induced by laser-irradiated Toxoplasma tachyzoites (LIT).


METHODS
ICR mice were immunized with LIT. After one month, the mice were challenged with live Toxoplasma tachyzoites to observe the effect of LIT. Indirect immunofluorescence assay and ELISA were used to detect CD4+/CD8+ lymphocyte subpopulation and IgG antibody.


RESULTS
Immunization with LIT could partially protect the mice from Toxoplasma infection, prolong the survival time, enhance the CD4+/CD8+ ratio and produce specific IgG antibody.


CONCLUSIONS
Intraperitoneal injection with LIT can induce partial protective immunity and specifically elicit ICR mice to generate humoral and cell-mediated immune responses.
Lin
A
A
Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou 310013.
Lu
S
S
Chen
C
C
Li
S
S
Chen
R
R
Lu
X
X
Huang
F
F
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis; D007834:Lasers; D051379:Mice; D008813:Mice, Inbred ICR; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014122:Toxoplasma / Q000276:immunology* / Q000528:radiation effects; D014124:Toxoplasmosis, Animal / Q000276:immunology* / Q000517:prevention & control
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G