Transcription of ahpC, katG, and katE genes in Escherichia coli is regulated by polyamines: polyamine-deficient mutant sensitive to H2O2-induced oxidative damage.
Polyamines (putrescine and spermidine) are present in almost all living organisms and participate in numerous cellular processes. In this study, we report the protective roles of polyamines against hydrogen peroxide (H2O2)-induced oxidative stress. All of ahpC, katG, and katE genes, known to participate in the antioxidant defense mechanism against H2O2-induced stress in Escherichia coli, failed to induce in the absence of polyamines during normal aerobic growth. The induction of both oxyR and rpoS gene expression, whose products are essential to induce ahpC, katG, and katE genes, was also absolutely dependent on polyamines. Polyamine-deficient E. coli mutant has increased susceptibility to exogenous H2O2, and this cell cytotoxicity was relieved to a wild-type level by addition of putrescine or spermidine (1mM), which restored the transcriptional induction of ahpC, katG, and katE genes. H2O2-removing capacity was measured in the mutant, showing a significantly low H2O2-removing capacity compared to the wild type when polyamines were not present. We concluded that the increased susceptibility of the polyamine-deficient E. coli mutant to H2O2 treatment resulted from an intracellular low level of H2O2-removing capacity through the failure of their regulons, ahpC, katG, and katE induction, as well as the failure of oxyR and rpoS induction.
Jung
Il Lae
IL
Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, 305-600, Yusong Taejon, Republic of Korea.
Kim
In Gyu
IG
D000975:Antioxidants / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics; D002374:Catalase / Q000235:genetics; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D008954:Models, Biological; D009154:Mutation; D018384:Oxidative Stress; D010544:Peroxidases / Q000235:genetics; D054464:Peroxiredoxins; D011073:Polyamines / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics; D012808:Sigma Factor / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
D000975:Antioxidants; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D011073:Polyamines; D012097:Repressor Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C499299:oxyR protein, E coli; C069567:sigma factor KatF protein, Bacteria; D006861:Hydrogen Peroxide; D010544:Peroxidases; C102664:hydroperoxidase II; D054464:Peroxiredoxins; C515937:ahpC protein, E coli; D002374:Catalase; C051253:katG protein, E coli