[Study on metabolites on aconitine in rabbit urine].
OBJECTIVE
To identify the main metabolites of aconitine in the urine of rabbits.


METHODS
After oral administration of aconitine (5 mg.kg-1), the urine of male rabbits was collected and extracted by solid phase extraction and analyzed by liquid chromatography-ion trap mass spectrometry.


RESULTS
Aconitine and 4 metabolites were found in the rabbit urine. Their protonated molecular ions at m/z 632, m/z 604, m/z 590, m/z 500 and multistage fragment ions with neutral loss of 60 u, 32 u, 28 u and 18 u were monitored. Their relative concentration were M1 > Aconitine > M4 > M2 > M3.


CONCLUSIONS
The metabolites M1-M4 were deduced as 16-O-demethylaconitine, benzoylaconine, 16-O-demethylbenzoylaconine and aconine, respectively.
Sun
Ying
Y
Department of Natural Pharmaceutical Chemistry, School of Pharmacy, jilin University, Changchun 130021, China.
Zhang
Hong-gui
HG
Shi
Xiang-guo
XG
Duan
Ming-yu
MY
Zhong
Da-fang
DF
D000157:Aconitine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine*; D000470:Alkaloids / Q000652:urine; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008297:Male; D011817:Rabbits; D021241:Spectrometry, Mass, Electrospray Ionization
C486136:16-O-demethylaconitine; D000470:Alkaloids; C047307:benzoylaconine; C047306:aconine; D000157:Aconitine