Actomyosin motility on nanostructured surfaces.
We have here, for the first time, used nanofabrication techniques to reproduce aspects of the ordered actomyosin arrangement in a muscle cell. The adsorption of functional heavy meromyosin (HMM) to five different resist polymers was first assessed. One group of resists (MRL-6000.1XP and ZEP-520) consistently exhibited high quality motility of actin filaments after incubation with HMM. A second group (PMMA-200, PMMA-950, and MRI-9030) generally gave low quality of motility with only few smoothly moving filaments. Based on these findings electron beam lithography was applied to a bi-layer resist system with PMMA-950 on top of MRL-6000.1XP. Grooves (100-200nm wide) in the PMMA layer were created to expose the MRL-6000.1XP surface for adsorption of HMM and guidance of actin filament motility. This guidance was quite efficient allowing no U-turns of the filaments and approximately 20 times higher density of moving filaments in the grooves than on the surrounding PMMA.
Bunk
Richard
R
Division of Solid State Physics and The Nanometer Consortium, University of Lund, Sweden.
Klinth
Jeanna
J
Montelius
Lars
L
Nicholls
Ian A
IA
Omling
Pär
P
Tågerud
Sven
S
Månsson
Alf
A
D008841:Actin Cytoskeleton / Q000502:physiology; D000205:Actomyosin / Q000502:physiology* / Q000648:ultrastructure; D000327:Adsorption; D004581:Electronics; D018625:Microscopy, Atomic Force; D009068:Movement; D036103:Nanotechnology; D011108:Polymers / Q000737:chemistry
D011108:Polymers; D000205:Actomyosin