Increased IFN-gamma protein in bronchoalveolar lavage fluid of anti-IP-10 antibody-treated F344 rats following Sendai viral infection.
A previous study showed that virus-resistant F344 rats had higher levels of interferon-gamma (IFN-gamma)-inducible protein 10 (IP-10) than did virus-susceptible BN rats early after Sendai viral infection. The initial goal of this study was to determine if an early high expression of IP-10 in F344 rats contributes to their resistance to virus-induced airway injury. Infected F344 rats were treated with anti-IP-10 rabbit serum or normal rabbit serum. Results indicated that blocking of IP-10 protein did not significantly change the resistance of F344 rats. However, we observed that neutralization of IP-10 increased IFN-gamma protein in bronchoalveolar lavage (BAL) fluid of F344 rats 7 days after inoculation compared with rats that received normal rabbit serum. The pulmonary IFN-gamma mRNA abundance remained comparable. This effect was not caused by fluctuation of the viral titer in the lung. This interesting phenomenon suggests that expression of IFN-gamma protein can be modulated by treatment with anti-IP-10 antibody at the posttranscriptional or translational level in this model.
Cai
Xuezhong
X
Department of Pathobiology, University of Florida, Gainesville, FL 32611-0880, USA.
Castleman
William L
WL
D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology*; D054357:Chemokine CXCL10; D019743:Chemokines, CXC / Q000276:immunology*; D004195:Disease Models, Animal; D007113:Immunity, Innate / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics; D008168:Lung / Q000821:virology; D009500:Neutralization Tests; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D051381:Rats; D011914:Rats, Inbred BN; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D010253:Respirovirus Infections / Q000276:immunology*; D029082:Sendai virus / Q000302:isolation & purification; D013045:Species Specificity
D000906:Antibodies; D054357:Chemokine CXCL10; D019743:Chemokines, CXC; D012333:RNA, Messenger; D007371:Interferon-gamma