[Effects of epidermal growth factor on wound healing of penetrating keratoplasty in rabbits].
OBJECTIVE
To observe the effects of epidermal growth factor (EGF) on wound healing of the penetrating keratoplasty(PKP) in rabbits.


METHODS
Twelve rabbits (24 eyes) were stratified randomly divided into 6 groups and underwent penetrating keratic autoplasy. Liquid scintillation counter was used to measure the incorporation rate of 3H-TdR. Pressure-detector was used to survey the intensity of keratic wound healing. AgNORs staining was used to count the fibroblast cells at keratic wound. HE staining, VG staining, AgNORs staining and transmission electron microscopy were used to observe the morphological changes of keratic wound.


RESULTS
1 The intensity of EGF group on 8 days, 14 days and 21 days after PKP was significantly higher than that of the control group(P < 0.01). 2. The incorporation rate of 3H-TdR in wound healing the EGF group on 14 days and 21 days was obviously higher than that of the control group(P = 0.01). 3. The number of fibroblast cells of EGF were more than that of the control group(P < 0.01) in 8 days.


CONCLUSIONS
EGF can promote the wound healing in rabbits after PKP.
Dai
C
C
Department of Ophthalmology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Liu
X
X
D000818:Animals; D003315:Cornea / Q000503:physiopathology / Q000601:surgery; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005347:Fibroblasts / Q000473:pathology; D015948:Keratoplasty, Penetrating; D011817:Rabbits; D011897:Random Allocation; D016622:Silver Staining; D014945:Wound Healing / Q000187:drug effects*
D004815:Epidermal Growth Factor