[Preparation and characterization of McAbs against lactate dehydrogenase of Plasmodium falciparum].
OBJECTIVE
To prepare and characterize the monoclonal antibodies(McAbs) against lactate dehydrogenase of the Plasmodium falciparum(LDHp).


METHODS
BALB/c mice were immunized with purified recombinant LDHp and McAbs against LDHp were prepared according to the protocol of hybridoma technique. The McAbs were characterized by ELISA and Western blot analysis.


RESULTS
Two McAbs against LDHp antigen were obtained. Both McAbs were IgG2b. The titer of two McAbs(2A5, 1H10) in the ascites was 1:25,600 and 1:12,800, and in supernatant were 1:512,1:256 respectively. The result of ELISA indicated that two McAbs reacted only with P. falciparum, and did not react with normal human red blood cells, P. vivax, Toxoplasma gondii, Schistosoma japonicum. It is recognized 33 kDa protein which was defined as LDHp by Western blot analysis.


CONCLUSIONS
Two hybridoma cell lines secreting high titer of McAbs against LDHp with high specificity were established.
Wu
Y S
YS
Institute of Tropical Medicine, First Military Medical University, Guangzhou 510515.
Dong
W Q
WQ
Li
M
M
Gao
Y
Y
Bi
H X
HX
Li
Y J
YJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D006825:Hybridomas / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010963:Plasmodium falciparum / Q000201:enzymology* / Q000276:immunology
D000911:Antibodies, Monoclonal; D007770:L-Lactate Dehydrogenase