Cyanobacterial leader peptides for protein secretion.
The leader peptide of the major secreted protein PilA1 of the cyanobacterium Synechocystis sp. strain PCC 6803 and several artificial leader peptides have been used to study secretion of the reporter protein lichenase to the culture medium. The strains of Synechocystis carrying lichenase with the leader sequences of PilA and with the leader sequence of Slr2016 efficiently secreted the reporter protein. The artificial leader sequence that was characterized by the overall positive charge (as PilA1 and Slr2016 leaders) also allowed secretion. The artificial leader with negative charge, however, did not allow secretion of the reporter protein. Moreover, no secreted proteins have been isolated from this strain using conventional techniques for preparation of secreted proteins. These data suggest that the general secretion pathway in cyanobacteria, at least for pilins, recognizes the overall charge of the leader sequences, and operates in a sequence-non-specific manner.
Sergeyenko
Tatiana V
TV
Institute of Plant Physiology, Russian Academy of Science, Botanicheskaya Street 35, 127276, Moscow, Russia.
Los
Dmitry A
DA
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D003470:Culture Media / Q000737:chemistry; D000458:Cyanobacteria / Q000254:growth & development / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006026:Glycoside Hydrolases / Q000378:metabolism; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D003470:Culture Media; D021382:Protein Sorting Signals; D006026:Glycoside Hydrolases; C028020:licheninase