[Generation of Dendritic Cells from Human Cord Blood CD34(+) Cells and Their Activation of T Cell-Dependent Antitumor Immune Response In Vitro]
To induce the growth and differentiation of dendritic cells (DCs) from human cord blood, CD34(+) cells isolated from human cord blood by mini-MACS were cultured in a liquid culture system with rhSCF, rhGM-CSF, rhTNF-alpha and rhFL for 10 days. Then the induced cells were characterized by DC's morphological and phenotypic properties. In addition, they stimulated the proliferation of allogeneic T cells and possessed an efficient capacity for initiating T cell-dependent antitumor immune responses in vitro. It is concluded that mature DCs could be obtained from human cord blood CD34(+) cells.
Wang
Guan-Jun
GJ
Department of Hematology and Oncology, The First Clinical Hospital, Jilin University, Changchun 130021, China; Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
Xie
Li-Hua
LH
Wang
Xiao
X
Feng
Kai
K
Li
Liang
L
Pei
Xue-Tao
XT