[Effects of nao-yi-an granule on bcl-2 expression in the rat brain after experimental cerebral hemorrhage].
OBJECTIVE
To investigate the protective effects and mechanisms of the traditional Chinese medicine complex, nao-yi-an granule (NYA) on experimental intracerebral hemorrhage (ICH) in rats.


METHODS
ICH models anesthesized were induced by stereotaxical injection of 2 microliters normal saline containing 0.4 U bacterial collagenase(type VII) into the right globas pallidus(GP), and the protein expression of bcl-2 in the rat brain after ICH was detected with the immunohistochemistry method, with which the effects of NYA were observed.


RESULTS
Bcl-2 positive cells were found in the cerebral cortex and basal ganglia area after ICH. Immunoreactivity was localized in the cytoplasm as a fine granular precipitate. The levels of bcl-2 expression in the model group varied at different time-points, and reached the peak at 2 d. In the NYA group, the protein expression of bcl-2 was increased significantly both in the cortex and in the basal ganglia compared with those in the model group.


CONCLUSIONS
NYA can significantly upregulate the protein expression of bcl-2.
Zhang
Hua-xian
HX
Institute of Combined Traditional Chinese Medicine and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.
Li
Xing-qun
XQ
Tang
Tao
T
D000818:Animals; D001921:Brain / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D020300:Intracranial Hemorrhages / Q000235:genetics / Q000378:metabolism*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D019253:Proto-Oncogene Proteins c-bcl-2