The relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies, Mn-SOD and carotenoids in rural Japanese residents.
This study investigated the relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies (oLAB), Mn-SOD, and carotenoids. Subjects were 79 males (mean age +/- standard deviation; 62.1 +/- 10.0 years) and 79 females (60.3 +/- 10.3 y) who attended a health examination screening in the town of Hokkaido, Japan. Serum 8-OHdG, Mn-SOD, and oLAB levels were measured by ELISA and serum carotenoids levels were measured by HPLC. Smoking habits were assessed by public health nurses using a questionnaire. Serum 8-OHdG levels were significantly higher in males than in females. On the other hand, serum levels of beta-carotene, alpha-carotene, beta-cryptoxanthin, and zeaxanthin and lutein were significantly lower in males than in females. Serum beta-carotene, beta-cryptoxanthin, and zeaxanthin and lutein were significantly lower in males who were current smokers, compared to non-smokers. Serum 8-OHdG levels were also significantly higher in current smokers. Furthermore, in males, serum oLAB and beta-carotene levels were significantly and negatively correlated with the number of cigarettes smoked per day. Serum Mn-SOD levels were unrelated to smoking habits in males. In conclusion, this preliminary study suggests that cigarette smoking increases serum 8-OHdG levels and reduces serum levels of oLAB and carotenoids, such as beta-carotene, beta-cryptoxanthin, and zeaxanthin and lutein in healthy male subjects. Serum levels of 8-OHdG, oLAB, and carotenoids may be useful biomarkers of oxidative conditions affected by smoking.
Suzuki
Koji
K
Department of Public Health, Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan.
Ito
Yoshinori
Y
Ochiai
Junichi
J
Aoki
Kunio
K
Wakai
Kenji
K
Tamakoshi
Akiko
A
Ando
Masahiko
M
Watanabe
Yoshiyuki
Y
Ozasa
Kotaro
K
Seki
Nao
N
Nishino
Yoshikazu
Y
Kondo
Takaaki
T
Ohno
Yoshiyuki
Y
Tamakoshi
Akiko
A
Mori
Mitsuru
M
Motohashi
Yutaka
Y
Tsuji
Ichiro
I
Nakamura
Yosikazu
Y
Iso
Hiroyasu
H
Mikami
Haruo
H
Hashimoto
Shuji
S
Inaba
Yutaka
Y
Hoshiyama
Yoshiharu
Y
Suzuki
Hiroshi
H
Shimizu
Hiroyuki
H
Toyoshima
Hideaki
H
Tokudome
Shinkan
S
Ito
Yoshinori
Y
Kikuchi
Shogo
S
Koizumi
Akio
A
Kawamura
Takashi
T
Watanabe
Yoshiyuki
Y
Miki
Tsuneharu
T
Date
Chigusa
C
Sakata
Kiyomi
K
Nose
Takayuki
T
Hayakawa
Norihiko
N
Yoshimura
Takesumi
T
Fukuda
Katsuhiro
K
Okamoto
Naoyuki
N
Shio
Hideo
H
Ohno
Yoshiyuki
Y
Kitagawa
Tomoyuki
T
Kuroki
Toshio
T
Tajima
Kazuo
K
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000704:Analysis of Variance; D002338:Carotenoids / Q000097:blood*; D016009:Chi-Square Distribution; D002851:Chromatography, High Pressure Liquid; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D012907:Smoking / Q000097:blood* / Q000453:epidemiology; D013482:Superoxide Dismutase / Q000097:blood*; D011795:Surveys and Questionnaires
D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D002338:Carotenoids; D000080242:8-Hydroxy-2'-Deoxyguanosine; D013482:Superoxide Dismutase; D003849:Deoxyguanosine