title: [Cloning of a species-specific gene fragment from Cryptosporidium parvum and the development of diagnostic PCR primers].
abstract: OBJECTIVE
  To develop a pair of diagnostic PCR primers for Cryptosporidium parvum.
  
  
  METHODS
  A species-specific gene fragment of C. parvum was obtained through RAPD analysis. After the fragment was isolated, purified, cloned and sequenced, a pair of primers FF was designed and synthesised based on the sequence. With the primers, the anticipated fragment in size of 603 bp was amplified by PCR from 2 American strains and 4 Chinese strains of C. parvum. The samples of 35 rabbits feces and 55 human feces were detected by PCR with primers FF and 021, the latter was a species-specific diagnostic primer reported by Morgan.
  
  
  RESULTS
  All six strains amplified by the primers FF showed same detection rate with 021. Sensitivity test indicated that DNA of 1 oocyst per gram of feces could be detected by the PCR.
  
  
  CONCLUSIONS
  The primers FF showed high specificity and sensitivity, and can be used for diagnosing Crytosporidium parvum infection.
authors.affiliation: Department of Preventive Veterinary Science, the Quartermarster University of PLA, Changchun 130062.
authors.forename: Zong-cheng
authors.initials: ZC
authors.lastname: Tian
authors.forename: Xi-chen
authors.initials: XC
authors.lastname: Zhang
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Li
authors.forename: Ji-gang
authors.initials: JG
authors.lastname: Yin
authors.forename: Ju
authors.initials: J
authors.lastname: Yang
authors.forename: Hong-xuan
authors.initials: HX
authors.lastname: He
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D003001:Cloning, Molecular; D003457:Cryptosporidiosis / Q000175:diagnosis*; D016785:Cryptosporidium parvum / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis*; D004285:Dogs; D004372:Ducks; D005243:Feces / Q000469:parasitology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011817:Rabbits; D019105:Random Amplified Polymorphic DNA Technique; D012377:Rodentia; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan