title: [Allele specific amplification for CYP2D6 gene related to intermediate metabolizer in Chinese subjects].
abstract: OBJECTIVE
  To establish an allele specific PCR amplification (ASA-PCR) for determination of the genotype of CYP2D6* 10B polymorphism in Chinese subjects.
  
  
  METHODS
  CYP2D6* 10B alleles of 65 healthy Chinese subjects were analyzed by a two-step PCR assay and the correlation of genotype and phenotype was studied.
  
  
  RESULTS
  There were 20 CYP2D6* 10B heterozygous genotypes subjects (wt/m) in 35 very extensive metabolizers (VEMs), which consisted the major part of VEM subjects (57%). Meanwhile, 20 subjects consisting 69% of 29 intermediate metabolizers were CYP2D6* 10B homozygous mutant genotypes (m/m). The poor metabolizer was also m/m. The metabolic ratio of CYP2D6* 10B m/m subjects were larger than wt/m and wild type, the values were -1.49 +/- 0.54, -2.20 +/- 0.49 and -2.47 +/- 0.61 (P < 0.01).
  
  
  CONCLUSIONS
  PCR-ASA was shown to be a rapid and specific method. It can be used to study the genetic polymorphism, especially CYP2D6 intermediate metabolism.
authors.affiliation: Department of Clinical Pharmacology, Nanjing General Hospital of Nanjing Command, Nanjing 210002, China. chenbing@jlonline.com
authors.forename: B
authors.initials: B
authors.lastname: Chen
authors.forename: W M
authors.initials: WM
authors.lastname: Cai
authors.forename: S S
authors.initials: SS
authors.lastname: Ling
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People; D002681:China; D019389:Cytochrome P-450 CYP2D6 / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D019389:Cytochrome P-450 CYP2D6