[Aspirin inhibited the adhesion of platelets to neutrophil in patients with acute myocardiac infarction].
OBJECTIVE
To investigate the effect and mechanism of aspirin(ASA) on platelet-neutrophil adhesion.


METHODS
Using rosette forming assay to observe the effect of ASA on the binding of platelets to neutrophil and radioimmunoassay to observe the effect of ASA on the thrombin-induced expression of GMP-140 on the surface of human platelets.


RESULTS
Patients with acute myocardial infarction showed a significant increase in neutrophil-platelet adhesion (63.3 +/- 7.8)%, (n = 20, P < 0.001) as compared with normal subjects(16.5 +/- 2.6)% (n = 20). Thrombin promoted adhesion by increasing the expression of GMP-140 on the surface of human platelets. When the concentration of thrombin was 0.5 U/ml, the rate of neutrophil-platelet adhesion and the number of molecular of GMP-140 on the surface of platelet was (64.2 +/- 4.2)%, and (1.80 +/- 0.20) x 10(3) per platelet respectively. At high concentration, ASA significantly inhibited thrombin (0.5 U/ml) stimulated platelets binding to neutrophils and expressing GMP-140 on their surface. When the final concentration of ASA was 500,5000 micrograms/ml, the ratio of thrombin-stimulated platelets binding neutrophils was (34.7 +/- 3.8)%, (21.2 +/- 3.6)% respectively (n = 20, P < 0.01); the number of molecular of GMP-140 expressing on the surface of platelet was (1.02 +/- 0.24) x 10(3), (0.68 +/- 0.18) x 10(3) per platelet respectively (n = 9, P < 0.001).


CONCLUSIONS
It contributes to the antithrombosis of ASA that inhibiting platelet-neutrophil adhesion by down expression of GMP-140 on the surface of human platelets.
Gao
C
C
Department of Pharmacology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Yu
X
X
Lin
Y
Y
Wang
Z
Z
D001241:Aspirin / Q000494:pharmacology*; D001792:Blood Platelets / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006801:Humans; D009203:Myocardial Infarction / Q000097:blood*; D009504:Neutrophils / Q000502:physiology*; D019007:P-Selectin / Q000378:metabolism; D010973:Platelet Adhesiveness / Q000187:drug effects
D005343:Fibrinolytic Agents; D019007:P-Selectin; D001241:Aspirin