Inhibition of Japanese encephalitis virus maturation and transport in PS cells to cell surface by brefeldin A.
A brefeldin A (BFA) treatment of porcine stable kidney (PS) cells resulted in inhibition of Japanese encephalitis virus (JEV) maturation and its transport to the cell surface. Intrestingly, the antigenicity of the virus, in contrast, remained unaffected as no difference in epitope presentation/expression was observed in BFA-treated and control (untreated) infected cells even though in the former cells a loss of hemagglutinating (HA) activity was recorded. Thus it seems that the BFA treatment did not affect the glycoprotein E (gpE) synthesis and folding essentially required for the epitope presentation/expression in cells.
Lad
V J
VJ
National Institute of Virology, Indian Council of Medical Research, 20-A, Dr. Ambedkar Road, P.B. No. 11, Pune 411 001, India.
Gupta
A K
AK
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000956:Antigens, Viral / Q000032:analysis / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D020126:Brefeldin A / Q000494:pharmacology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004664:Encephalitis Virus, Japanese / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology / Q000378:metabolism; D051379:Mice; D013552:Swine
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D020126:Brefeldin A