Assembly and overexpression of membrane proteins in Escherichia coli.
The bacterium Escherichia coli is one of the most popular model systems to study the assembly of membrane proteins of the so-called helix-bundle class. Here, based on this system, we review and discuss what is currently known about the assembly of these membrane proteins. In addition, we will briefly review and discuss how E. coli has been used as a vehicle for the overexpression of membrane proteins.
Drew
David
D
Department of Biochemistry and Biophysics, Stockholm University, S-106 91 Stockholm, Sweden.
Fröderberg
Linda
L
Baars
Louise
L
de Gier
Jan-Willem L
JW
D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D007425:Intracellular Membranes / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D018271:Signal Recognition Particle / Q000378:metabolism
D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D018271:Signal Recognition Particle