title: Fission yeast synaptobrevin is involved in cytokinesis and cell elongation.
abstract: Synaptobrevin is a vesicle-associated membrane protein playing an essential role in regulated vesicle transport. In this study, we characterized Syb1, synaptobrevin of Schizosaccharomyces pombe. Syb1 was located on various sizes of vesicle-like structures in the cytoplasm and enriched in the medial region and cell ends. Transport of Syb1 to the medial region was mainly dependent on F-actin and Myo52/Myo4. Syb1 is essential for cell viability and most of the syb1-null cells showed a round or short cylindrical form. These results suggest that Syb1 is involved in membrane trafficking of cytokinesis and cell elongation.
authors.affiliation: Graduate School of Arts and Sciences, University of Tokyo, Japan. cedam@mail.ecc.u-tokyo.ac.jp
authors.forename: Masaki
authors.initials: M
authors.lastname: Edamatsu
authors.forename: Yoko Y
authors.initials: YY
authors.lastname: Toyoshima
mesh_terms: D000595:Amino Acid Sequence; D002455:Cell Division; D048429:Cell Size; D022162:Cytoplasmic Vesicles / Q000737:chemistry; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry / Q000502:physiology*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D050683:R-SNARE Proteins; D012568:Schizosaccharomyces / Q000737:chemistry / Q000166:cytology*; D029702:Schizosaccharomyces pombe Proteins / Q000032:analysis / Q000737:chemistry / Q000502:physiology*; D017386:Sequence Homology, Amino Acid
chemical_list: D008565:Membrane Proteins; D050683:R-SNARE Proteins; D029702:Schizosaccharomyces pombe Proteins; C472866:Syb1 protein, S pombe