[Experimental study on the potential carcinogenicity of cooking oil fume condensate].
The potential carcinogenicity of cooking oil fume condensate (COFC) to human was studied. Human embryo lung diploid fibroblast cell strain KMB-17 cell was applied to establish a human diploid cell transformation system in vitro. Different concentrations of COFC were added into the media and co-incubated with cells. The malignant degree of transformation was assessed by the biological characteristics of the cells. The concentrations of COFC within the dose range of the experiment could induce the malignant transformation of KMB-17 cell, and with a obvious dose-response relationship (r = 0.9811). Transformed cells have exhibited many characteristics associating with malignant transformation, such as loss of density and contact-dependent inhibition, growth at low serum concentration, agglutination by low concentration of Con A, alteration of karyotype from diploid to aneuploid, and lose of anchorage dependence. It suggested that the malignant transformation of human embryo lung diploid fibroblast cell strain KMB-17 cell could be induced by COFC, which might have potential carcinogenicity to human.
Zhao
Jinming
J
School of Public Health, Nanhua University, Hengyang 421001, China.
Su
Fei
F
Zhou
Shaoqin
S
D000393:Air Pollutants / Q000633:toxicity*; D016902:Air Pollution, Indoor / Q000009:adverse effects*; D002273:Carcinogens / Q000633:toxicity; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003296:Cooking; D004042:Dietary Fats, Unsaturated / Q000633:toxicity; D004622:Embryo, Mammalian; D006801:Humans; D008168:Lung / Q000166:cytology; D010938:Plant Oils / Q000633:toxicity; D012906:Smoke / Q000009:adverse effects*
D000393:Air Pollutants; D002273:Carcinogens; D004042:Dietary Fats, Unsaturated; D010938:Plant Oils; D012906:Smoke