[Observation on the dynamics of specific anti-GST antibodies in rabbits immunized with recombinant 26 kDa GST of Schistosoma japonicum].
OBJECTIVE
To investigate the dynamics of the anti-GST antibodies in rabbits immunized with recombinant 26 kDa GST of Schistosoma japonicum.


METHODS
The specific antibodies were detected weekly by GST-ELISA in rabbits vaccinated with purified recombinant 26 kDa GST antigen of S. japonicum plus FCA/IFCA; rabbits immunized with 0.85% saline plus FCA/IFCA served as control group.


RESULTS
The specific anti-GST antibodies began to increase at week 4 post-immunization, the mean OD value in rabbits vaccinated with recombinant GST could reach 1.03 +/- 0.46 (adjuvant control group only 0.42 +/- 0.04). The experiment was completed at week 65 post-immunization when the mean OD values for specific anti-GST antibody in immunized rabbits were 0.94 +/- 0.26(0.29 +/- 0.16 in the control group).


CONCLUSIONS
Strong anti-GST antibody responses could be induced in rabbits vaccinated with recombinant 26 kDa GST of S. japonicum.
Xu
Y
Y
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Song
G
G
Liu
S
S
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005982:Glutathione Transferase / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D012549:Schistosoma japonicum / Q000201:enzymology / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D011994:Recombinant Proteins; D005982:Glutathione Transferase