The study on culture and ultrastructure of glaucomatous trabecular cells in vitro.
OBJECTIVE
To establish the culture system of human glaucomatous trabecular cells in vitro and study their ultrastructures.


METHODS
The trabecular specimens from trabeculectomy were cultured in vitro and passaged 3 times, then identified. Moreover, the glaucomatous cells were observed with electron microscope while compared with the normal ones.


RESULTS
Cultured human glaucomatous trabecular cells were obtained. The ultrastructure of the cells showed the decrease in vilious project, coated vesicle and lysosomal inclusion.


CONCLUSIONS
The establishment of human glaucomatous trabecular cells culture in vitro made the culture system more perfect. The morphologic changes might be related to the abnormal functions of human trabecular meshwork cells.
Ge
J
J
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Lin
M
M
Zheng
J
J
Zhuo
Y
Y
D002455:Cell Division; D002478:Cells, Cultured; D003907:Dexamethasone; D005902:Glaucoma, Open-Angle / Q000139:chemically induced / Q000473:pathology*; D006801:Humans; D014129:Trabecular Meshwork / Q000166:cytology / Q000648:ultrastructure*
D003907:Dexamethasone