title: Transcription factor IID recruitment and Sp1 activation. Dual function of TAF1 in cyclin D1 transcription.
abstract: Cyclin D1 is an oncogene that regulates progression through the G(1) phase of the cell cycle. A temperature-sensitive missense mutation in the transcription factor TAF1/TAF(II)250 induces the mutant ts13 cells to arrest in late G(1) by decreasing transcription of cell cycle regulators, including cyclin D1. Here we provide evidence that TAF1 serves two independent functions, one at the core promoter and one at the upstream activating Sp1 sites of the cyclin D1 gene. Using in vivo genomic footprinting, we have identified protein-DNA interactions within the cyclin D1 core promoter that are disrupted upon inactivation of TAF1 in ts13 cells. This 33-bp segment, which we termed the TAF1-dependent element 1 (TDE1), contains an initiation site that displays homology to the consensus motif and is sufficient to confer a requirement for TAF1 function. Electrophoretic mobility shift assays reveal that binding of ts13-TAF1-containing TFIID complexes to the cyclin D1 TDE1 occurs at 25 degrees C but not at 37 degrees C in vitro and involves the initiator element. Temperature-dependent DNA binding activity is also observed for TAF1-TAF2 heterodimers assembled with the ts13 mutant but not the wild-type TAF1 protein. These data suggest that a function of TAF is required for the interaction of TFIID with the cyclin D1 initiator. Our finding that recruitment of TFIID, by insertion of a TBP binding site upstream of the TDE1, restores basal but not activated transcription supports the model that TAF1 carries out two independent functions at the cyclin D1 promoter.
authors.affiliation: University of Washington, Department of Pharmacology, School of Medicine, Health Sciences Center, Box 357280, Seattle, Washington 98195-7280, USA.
authors.forename: Traci L
authors.initials: TL
authors.lastname: Hilton
authors.forename: Edith H
authors.initials: EH
authors.lastname: Wang
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D006224:Cricetinae; D019938:Cyclin D1 / Q000235:genetics* / Q000378:metabolism; D018983:DNA Footprinting; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000502:physiology*; D007668:Kidney; D008969:Molecular Sequence Data; D011033:Podophyllin / Q000031:analogs & derivatives* / Q000378:metabolism*; D011034:Podophyllotoxin / Q000031:analogs & derivatives; D011401:Promoter Regions, Genetic; D021122:Protein Subunits / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D035362:Transcription Factor TFIID / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection
chemical_list: D020121:5' Untranslated Regions; D002868:Chromosomal Proteins, Non-Histone; D017931:DNA Primers; D021122:Protein Subunits; D011994:Recombinant Proteins; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019938:Cyclin D1; C003170:mitopodozide; D011033:Podophyllin; D011034:Podophyllotoxin