Polyamines protect Escherichia coli cells from the toxic effect of oxygen.
Wild-type Escherichia coli cells grow normally in 95% O(2)/5% CO(2). In contrast, cells that cannot make polyamines because of mutations in the biosynthetic pathway are rapidly killed by incubation in 95% O(2)/5% CO(2). Addition of polyamines prevents the toxic effect of oxygen, permitting cell survival and optimal growth. Oxygen toxicity can also be prevented if the growth medium contains an amino acid mixture or if the polyamine-deficient cells contain a manganese-superoxide dismutase (Mn-SOD) plasmid. Partial protection is afforded by the addition of 0.4 M sucrose or 0.4 M sorbitol to the growth medium. We also report that concentrations of H(2)O(2) that are nontoxic to wild-type cells or to mutant cells pretreated with polyamines kill polyamine-deficient cells. These results show that polyamines are important in protecting cells from the toxic effects of oxygen.
Chattopadhyay
Manas K
MK
Laboratory of Biochemistry and Genetics, Building 8, Room 223, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Tabor
Celia White
CW
Tabor
Herbert
H
D000388:Air; D000588:Amines / Q000737:chemistry; D002103:Cadaverine / Q000494:pharmacology; D002455:Cell Division; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism* / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009154:Mutation; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D011073:Polyamines / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D011700:Putrescine / Q000494:pharmacology; D013012:Sorbitol / Q000737:chemistry / Q000494:pharmacology; D013095:Spermidine / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology; D013997:Time Factors
D000588:Amines; D011073:Polyamines; D013012:Sorbitol; D013395:Sucrose; D004247:DNA; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D002103:Cadaverine; D010100:Oxygen; D013095:Spermidine; D011700:Putrescine