title: Endotoxin decreases serum IGFBP-3 and liver IGFBP-3 mRNA: comparison between Lewis and Wistar rats.
abstract: We studied the IGFBP-3 response to endotoxin, in Wistar and Lewis rats. Compared to Wistar rats, Lewis rats have a reduced adrenal and IGF-I response to inflammatory stimuli. Rats received two injections of 1 mg/kg of lipopolysaccharide (LPS) and were killed 4 h after the second injection. LPS decreased serum concentrations of GH in Wistar (P<0.05), but not in Lewis rats. However, serum IGFBP-3 was decreased both in Wistar and in Lewis rats. Furthermore, LPS administration decreased IGFBP-3 gene expression in the liver in both rat strains (P<0.01). Lewis rats had lower serum IGFBP-3 than Wistar rats (P<0.01). This difference could be secondary to the increased IGFBP-3 proteolysis in serum observed in Lewis rats. These data indicate that acute inflammation inhibits serum concentrations of IGFBP-3 by decreasing its synthesis in the liver, rather than increasing its proteolysis. This effect seems to be GH and IGF-I independent.
authors.affiliation: Departmento de Fisiología, Facultad de Medicina, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Priego
authors.forename: I
authors.initials: I
authors.lastname: Ibáñez de Cáceres
authors.forename: A I
authors.initials: AI
authors.lastname: Martín
authors.forename: M A
authors.initials: MA
authors.lastname: Villanúa
authors.forename: A
authors.initials: A
authors.lastname: López-Calderón
mesh_terms: D000818:Animals; D010450:Endopeptidases / Q000378:metabolism; D007249:Inflammation / Q000097:blood; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000096:biosynthesis / Q000097:blood / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D051381:Rats; D011917:Rats, Inbred Lew / Q000378:metabolism*; D017208:Rats, Wistar / Q000378:metabolism*
chemical_list: D018972:Insulin-Like Growth Factor Binding Protein 3; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D010450:Endopeptidases