[Depressive effect of tetrandrine on collagen synthesis in vascular smooth muscle cells].
OBJECTIVE
To clarify whether collagen deposition and collagen synthesis are increased in renovascular hypertensive rats (RHR) and whether these are affected by treatment with tetrandrine (Tet).


METHODS
Collagen deposition was detected by electron microscope and evaluated by the amount of hydroxyproline in aorta of sham-operated rats (Sham), renovascular hypertensive rats (RHR), and Tet treated RHR. The aortic vascular smooth muscle cells (AVSMCs) were isolated and cultured. The collagen synthesis in cultured AVSMCs was detected by [3H]-proline incorporation.


RESULTS
The ultrastructure of aorta suggests that AVSMCs in RHR had been transferred from contractile phenotype to synthetic phenotype. Compared with sham-operated rats, the hydroxyproline content in aorta was increased by 36.6% in RHR, while it was reduced by 18.7% in Tet treated RHR (P < 0.01, vs RHR). Tet (0.1-10 mumol.L-1) treatment in vitro induced a concentration dependent depression [3H]-proline incorperation stimulated by NE or Ang II in AVSMCs from either Sham or RHR.


CONCLUSIONS
The depressive effects of tetrandrine on collagen synthesis and deposition in arteries may contribute to the regression of vascular remodeling.
Li
Q P
QP
Department of Cardiovascular Pharmacology, Nanjing Medical University, Nanjing 210029, China. qpli@njmu.edu.cn
Lu
Z A
ZA
Rao
M R
MR
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001013:Aorta, Thoracic / Q000473:pathology; D044182:Benzylisoquinolines / Q000494:pharmacology*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D015870:Gene Expression / Q000187:drug effects*; D006909:Hydroxyproline / Q000096:biosynthesis; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000470:Alkaloids; D000959:Antihypertensive Agents; D044182:Benzylisoquinolines; C009438:tetrandrine; D003094:Collagen; D006909:Hydroxyproline