Membrane properties related to the firing behavior of zebrafish motoneurons.
The physiological and pharmacological properties of the motoneuron membrane and action potential were investigated in larval zebrafish using whole cell patch current-clamp recording techniques. Action potentials were eliminated in tetrodotoxin, repolarized by tetraethylammonium (TEA) and 3,4-diaminopyridine (3,4-AP)-sensitive potassium conductances, and had a cobalt-sensitive, high-threshold calcium component. Depolarizing current injection evoked a brief (approximately 10-30 ms) burst of action potentials that was terminated by strong, outwardly rectifying voltage-activated potassium and calcium-dependent conductances. In the presence of intracellular cesium ions, a prolonged plateau potential often followed brief depolarizations. During larval development (hatching to free-swimming), the resting membrane conductance increased in a population of motoneurons, which tended to reduce the apparent outward rectification of the membrane. The conductances contributing to action potential burst termination are hypothesized to play a role in patterning the synaptically driven motoneuron output in these rapidly swimming fish.
Buss
Robert R
RR
McGill Centre for Research in Neuroscience and Department of Biology, McGill University, Montreal, H3G 1A4 Quebec, Canada.
Bourque
Charles W
CW
Drapeau
Pierre
P
D015761:4-Aminopyridine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000077770:Amifampridine; D000779:Anesthetics, Local / Q000494:pharmacology; D000818:Animals; D015220:Calcium Channels / Q000502:physiology; D003035:Cobalt / Q000494:pharmacology; D009046:Motor Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D026902:Potassium Channel Blockers / Q000494:pharmacology; D015221:Potassium Channels / Q000502:physiology; D015222:Sodium Channels / Q000502:physiology; D013550:Swimming / Q000502:physiology; D019789:Tetraethylammonium / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D015027:Zebrafish
D000779:Anesthetics, Local; D015220:Calcium Channels; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D015222:Sodium Channels; D003035:Cobalt; D013779:Tetrodotoxin; D019789:Tetraethylammonium; D015761:4-Aminopyridine; D000077770:Amifampridine