[Effect of excessive Zn2+ and deficient Zn2+ on the expression of wild type-p53 mRNA in rat glicoma cells].
RT-PCR is applied to observe the effects of excessive and deficient Zn2+ on the expression of wild type-p53 (wt-p53) in rat glicoma cells. Rat glicoma cells in garithmic growth phase is treated with 0.3 mmol/L Zn2+ and 5 mumol/L TPEN respectively for 24 hours. Results show that the expression of wt-p53 of rat glicoma cells increased obviously in the deficient zinc group but does not increased in the excessive zinc group. These indicate that the expression of wt-p53 mRNA of rat glicoma cells is up-regulated by deficient zinc and inhibited by excessive zinc.
Li
Bo
B
Department of Nutrition and Food Hygiene, Fourth Military Medical University, Xi'an 710033, China.
Wang
Feng
F
Luo
Wenjing
W
Yang
Ruihua
R
D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism; D005910:Glioma / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics; D015032:Zinc / Q000494:pharmacology*
D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D015032:Zinc