title: MSI-99, a magainin analogue, imparts enhanced disease resistance in transgenic tobacco and banana.
abstract: Magainin is one of the earliest reported antimicrobial peptides isolated from skin secretions of the African clawed frog Xenopus laevis. A synthetic substitution analogue of magainin, MSI-99, is employed in this study to impart disease resistance in transgenic tobacco ( Nicotiana tabacumL.) and banana [( Musaspp. cv. Rasthali (AAB)]. This peptide inhibited the growth and spore germination of Fusarium oxysporumf.sp. cubenseat 16 micro g/ml. MSI-99 has been subcloned into plant expression vectors pMSI164 and pMSI168, targeting the peptide into the cytoplasm and extracellular spaces, respectively. Tobacco plants transformed with pMSI168 showed enhanced resistance against Sclerotinia sclerotiorum, Alternaria alternataand Botrytis cinerea. Transgenic banana pants were obtained for both pMSI164 and pMSI168 transformations and showed resistance to F. oxysporumf.sp. cubenseand Mycosphaerella musicola. The transgenic nature of the transformants and expression of this peptide was confirmed through polymerase chain reaction (PCR) and reverse transcription (RT)-PCR. The results suggest that MSI-99 can be useful in imparting enhanced disease resistance in transgenic plants.
authors.affiliation: Nuclear Agriculture and Biotechnology DivisionBhabha Atomic Research Centre, 400085, Trombay, Mumbai, India.
authors.forename: A
authors.initials: A
authors.lastname: Chakrabarti
authors.forename: T R
authors.initials: TR
authors.lastname: Ganapathi
authors.forename: P K
authors.initials: PK
authors.lastname: Mukherjee
authors.forename: V A
authors.initials: VA
authors.lastname: Bapat
mesh_terms: D000528:Alternaria / Q000187:drug effects / Q000254:growth & development; D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology; D001203:Ascomycota / Q000187:drug effects / Q000254:growth & development; D020171:Botrytis / Q000187:drug effects / Q000254:growth & development; D003001:Cloning, Molecular; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development; D005670:Fusarium / Q000187:drug effects / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D028521:Musa / Q000187:drug effects* / Q000235:genetics / Q000382:microbiology; D010455:Peptides; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000494:pharmacology*; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins; D013170:Spores / Q000187:drug effects; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics / Q000382:microbiology; D019076:Transgenes / Q000235:genetics / Q000502:physiology
chemical_list: D000935:Antifungal Agents; C446524:MSI-99 peptide; D010455:Peptides; D010940:Plant Proteins; D011994:Recombinant Proteins