title: High antigenic cross-reaction among the bacterial species responsible for diseases of cultured freshwater fishes and strategies to overcome it for specific serodiagnosis.
abstract: Antigenic sharing among the most commonly bacterial pathogens such as Aeromonas hydrophila, Edwardsiella tarda and Pseudomonas fluorescens of Indian major carps has been studied using immunological reactions such as cross-agglutination, disc diffusion and indirect enzyme linked immunosorbent assay (ELISA). The data were analysed using statistical analysis (SAS), version 6.12. The results showed high antigenic similarities among the bacterial whole cells, whole cell lysates, somatic 'O' antigens, lipopolysaccharides (LPS) and extracellular products (ECP). However, few or no similarities were observed in ECP components of <20kD. The present study indicates a need to develop differential diagnostic methods based on serology by choosing the highly specific less cross-reactive ECP antigen.
authors.affiliation: Aquatic Animal Health Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Orissa, India. pswainy2k@yahoo.co.in
authors.forename: P
authors.initials: P
authors.lastname: Swain
authors.forename: S K
authors.initials: SK
authors.lastname: Nayak
authors.forename: A
authors.initials: A
authors.lastname: Sahu
authors.forename: P K
authors.initials: PK
authors.lastname: Meher
authors.forename: B K
authors.initials: BK
authors.lastname: Mishra
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology; D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D017756:Aquaculture; D015153:Blotting, Western / Q000662:veterinary; D002347:Carps; D003429:Cross Reactions; D003937:Diagnosis, Differential; D020609:Edwardsiella tarda / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005393:Fish Diseases / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D005779:Immunodiffusion / Q000662:veterinary; D008070:Lipopolysaccharides / Q000276:immunology; D011551:Pseudomonas fluorescens / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D008070:Lipopolysaccharides