[Effect of shenmai injection on neurocyte apoptosis and change of cytoplasmic calcium].
OBJECTIVE
To study the neurocyte apoptosis and change of cytosolic free calcium induced by hypoxia/hypoglycemia culture in SH-SY5Y human neuroblastoma cells, and the effect of Shenmai Injection (SMI) on them.


METHODS
The neurocyte apoptosis rate was analysed quantitatively by flow cytometry and cytosolic calcium was determined by measuring mean fluorescent value with Fluo-3 flurometry.


RESULTS
Hypoxia/hypoglycemia could induce neurocyte apoptosis and increase the cytosolic calcium in SH-SY5Y cells. SMI could inhibit the SH-SY5Y cell apoptosis and lower the concentration of cytosolic free calcium.


CONCLUSIONS
Apoptosis is one of the death pattern of nerve cells. SMI could obviously reduce the hypoxia/hypoglycemia induced apoptosis and calcium overload, thus protect the neurocytes.
He
L Y
LY
Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100700.
Sun
S L
SL
Fan
J P
JP
D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism*; D015687:Cell Hypoxia / Q000187:drug effects; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D009447:Neuroblastoma / Q000473:pathology; D009474:Neurons / Q000166:cytology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; C009252:fructus schizandrae, radix ginseng, radix ophiopogonis drug combination; D002118:Calcium