Determination of Tripamide in human urine by high-performance liquid chromatography and high-performance liquid chromatography/electrospray ionization tandem mass spectrometry.
Tripamide is a drug widely used in clinical practice for the treatment of hypertension and edema. This work evaluated a screening method for Tripamide and its urinary metabolites in human urine, using high-performance liquid chromatography diode-array detection (HPLC/DAD). Identification of these metabolites was investigated by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC/ESI-MS/MS) after dosing with 15 mg Tripamide. Acid hydrolysis showed that Tripamide is conjugated in the body. Two suspected metabolites were detected by HPLC/DAD. HPLC/ESI-MS/MS analysis suggested that these metabolites were probably hydroxylated together with loss of the -NH(2) group and dehydrogenation. These results will be useful in confirmation methods for Tripamide in doping control.
Kim
Yunje
Y
Doping Control Center, Korea Institute of Science and Technology, PO Box 131, Cheongryang, Seoul, Korea. yjkim@kistmail.kist.ac.kr
Hwang
Joungboon
J
Kim
Myungsoo
M
Lee
Won
W
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004232:Diuretics / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000378:metabolism / Q000652:urine*; D008297:Male; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D016482:Urinalysis / Q000379:methods
D004232:Diuretics; D007211:Indoles; C031282:tripamide