title: [Expression of Bcl-2 and P53 Gene Proteins on Leukemia Cells and Its Correlation with Chemotherapeutic Efficacy]
abstract: To explore Bcl-2 and P53 gene proteins expression on human leukemia cells and their relationship with chemotherapeutic efficacy, Bcl-2 and P53 gene proteins expression was assayed by ABC immunohistochemical staining. Results showed that the expression rates of Bcl-2 and P53 gene proteins were 67% and 41% respectively in leukemia cells from 52 patients. While there was no difference of Bcl-2 protein level in ALL and ANLL, the P53 protein level was higher in ANLL than that in ALL (P < 0.05). When CML patients got into the blast crisis phase, the level of Bcl-2 and P53 proteins became very high. Compare with previously untreated AL, relapse/refractory AL patients had higher Bcl-2 and P53 protein level, lower marrow complete remission, and was easy to relapse. The expression of Bcl-2 and P53 protein could be used as new predictors of chemotherapeutic efficacy and prognosis in patients with leukemia. The high protein expression of Bcl-2 and P53 demonstrated that CML was conversion to blast crisis phase.
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Dalian Medical University, Dalian 116023, China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: An
authors.forename: Zhenlan
authors.initials: Z
authors.lastname: Song
authors.forename: Xuebin
authors.initials: X
authors.lastname: Dong
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Wang
authors.forename: Shimei
authors.initials: S
authors.lastname: Lian