[Cloning and identification of deltamethrin-resistance or susceptibility associated genes of Culex pipiens pallens].
OBJECTIVE
To obtain deltamethrin-resistance or susceptibility associated genes of Culex pipiens pallens.


METHODS
The differentially expressed genes were obtained by suppression subtractive hybridization (SSH), and identified by cDNA microarray and reverse Northern blotting.


RESULTS
523 and 286 clones were selected respectively in the two directional SSH. 155 and 42 genes were respectively expressed 2-3 and > 3 times higher in the insecticide-resistant strain than in the susceptible strain; 15 and 9 genes were respectively expressed 2-3 and > 3 times higher in the susceptible strain than in the resistant strain. There were 2 genes only expressed in the insecticide-resistant strain. 51 three times differentially expressed clones and 2 specially expressed clones were sequenced. 44 sequences were obtained which belong to 13 new genes. There were 8 over-expressed genes in resistant strain, 7 of which were similar respectively to mitochondrion rRNA gene, 60S ribosomal protein gene, 40S ribosomal protein S4 gene, trypsin gene, chymotrypsin A gene, ospin gene, and 16S ribosomal RNA gene. There were 5 over-expressed genes in susceptible strain, 2 of them being similar with 40S ribosomal protein S29 gene and myosin regulatory light chain 2 gene. In addition, 2 genes specially expressed in resistant strain were similar respectively to glycogen branching enzyme gene and ribosomal protein 46 gene.


CONCLUSIONS
The differentially expressed genes may be associated with deltamethrin-resistance or susceptibility of Culex pipiens pallens.
Tian
H S
HS
Department of Pathobiology and Immunology, Nanjing Medical University, Nanjing 210029.
Zhu
C L
CL
Gao
X H
XH
Ma
L
L
Shen
B
B
Li
X L
XL
Wu
G L
GL
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003465:Culex / Q000187:drug effects / Q000235:genetics*; D020869:Gene Expression Profiling; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides / Q000494:pharmacology*; D009570:Nitriles; D020411:Oligonucleotide Array Sequence Analysis; D011722:Pyrethrins / Q000494:pharmacology*; D012689:Sequence Homology, Nucleic Acid
D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin