[Association of coagulation factor V, VII gene polymorphisms with coronary heart disease].
OBJECTIVE
To study the distribution of coagulation factor V(FV), VII(FVII) gene polymorphisms in Chinese Han population and the association of the polymorphisms with coronary heart disease(CHD).


METHODS
Genotypes of FV FVII were typed in 234 CHD patients and 210 controls by polymerase chain reaction-restriction fragment length polymorphism. Selected coronary angiography was performed in 234 CHD patients.


RESULTS
FVII allelic frequencies of R,Q and H7,H6 were 94.6%, 5.6%, 70.3%, 29.7% and 91.9%, 8.1%, 60.9%, 39.1% in CHD group and control group respectively. Genotype distribution was in accordance with Hardy-Weinberg equilibrium. There was no significant difference in frequencies of allele and genotype in R353Q or HVR4 polymorphisms between CHD group and control group. The distribution of allele and genotype in R353Q was of significant difference between non-myocardial infarction subgroup and myocardial infarction subgroup (chi2 = 4.711, P<0.05, OR=0.37,95% CI: 0.15-0.94). However, HVR4 polymorphism was not found to be of significant difference within two group (chi2 = 0.142, P>0.05). There was no FV Leiden mutation in all the CHD patients and normal controls.


CONCLUSIONS
The Q allele of the R353Q polymorphism of the FVII gene may be a protective factor against myocardial infarction.
Xu
Geng
G
Department of Cardiology, the Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, PR China.
Jin
Guo-dong
GD
Fu
Guo-sheng
GS
Ma
Ji
J
Shan
Jiang
J
D000368:Aged; D002681:China; D003327:Coronary Disease / Q000235:genetics* / Q000473:pathology; D004247:DNA / Q000235:genetics; D005165:Factor V / Q000235:genetics*; D005167:Factor VII / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
C095381:factor V Leiden; D005165:Factor V; D005167:Factor VII; D004247:DNA