[Micropatterning of biotin-avidin layers and cell location].
The located culture of cells on patterned surfaces is useful for tissue engineering, biosensor development and fundamental research of cell biology. It is presented here a rapid fabrication method of Biotin-Avidin protein layers micropattern, which is based on soft-lithography technology. The bovine aortic endothelial cells are cultured on the micropatterned surface. It is found that cell location can be controlled on the scale of individual cell by this method.
Hu
Jiang
J
Lab of Microgravity, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080, China.
Wang
Zhan-Hui
ZH
Tao
Zu-Lai
ZL
D000818:Animals; D001360:Avidin / Q000378:metabolism*; D001710:Biotin / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured / Q000166:cytology*; D023822:Tissue Engineering
D001360:Avidin; D001710:Biotin