title: [Two restriction fluorescence labeling methods for enhancing the signal-to-noise ratio of cDNA microarray hybridization].
abstract: OBJECTIVE
  To study the signal-to-noise ratio (SNR) of two restricted fluorescence labeling methods for examining gene expression profile by microarray hybridization.
  
  
  METHODS
  Samples of Saccharomyces cerevisiae mRNA was labeled by traditional reverse transcription method and 2 restriction fluorescent labeling methods using respectively Cy-universal primer and extension incorporated Cy-dNTP. The labeled samples were examined by the microarray, followed by washing and scanning under the same conditions.
  
  
  RESULTS
  The two restriction labeling methods showed superior results with lowered background and enhanced SNR and sensitivity, and Cy-universal primer labeling presented the best results.
  
  
  CONCLUSIONS
  SNR can be enhanced by the restriction labeling methods, which improve the applicability of microarray technology.
authors.affiliation: Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China. shirong@fimmu.edu.cn
authors.forename: Rong
authors.initials: R
authors.lastname: Shi
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Ma
authors.forename: Yan-bin
authors.initials: YB
authors.lastname: Song
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Cui-hua
authors.initials: CH
authors.lastname: Liu
authors.forename: Wen-ling
authors.initials: WL
authors.lastname: Zheng
mesh_terms: D005453:Fluorescence; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012441:Saccharomyces cerevisiae