title: Ischemic rat brain extracts induce human marrow stromal cell growth factor production.
abstract: Intravenous administration of human bone marrow stromal cells (hMSCs) after middle cerebral artery occlusion (MCAo) in rats provides functional benefit. We tested the hypothesis that these functional benefits are derived in part from hMSC production of growth and trophic factors. Quantitative sandwich enzyme-linked immunosorbent assay (ELISA) of hMSCs cultured with normal and MCAo brain extracts were performed. hMSCs cultured in supernatant derived from ischemic brain extracts increased production of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF). These neurotrophins and angiogenic growth factors increased in a post-ischemia time-dependent manner. The hMSC capacity to increase expression of growth and trophic factors may be the key to the benefit provided by transplanted hMSCs in the ischemic brain.
authors.affiliation: Department of Neurology, Division of Hematology/Medical Oncology, Henry Ford Health Sciences Center, Detroit, USA.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Mark
authors.initials: M
authors.lastname: Katakowski
authors.forename: Lijie
authors.initials: L
authors.lastname: Zhang
authors.forename: Jieli
authors.initials: J
authors.lastname: Chen
authors.forename: Yongxian
authors.initials: Y
authors.lastname: Xu
authors.forename: Subhash C
authors.initials: SC
authors.lastname: Gautam
authors.forename: Michael
authors.initials: M
authors.lastname: Chopp
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism; D017690:Cell Transplantation; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006133:Growth Substances / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D051381:Rats; D017154:Stromal Cells / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors; D014020:Tissue Extracts / Q000494:pharmacology*
chemical_list: D006133:Growth Substances; D014020:Tissue Extracts