[Expression of fused protein A-green fluorescent protein (PA-GFP)].
The green fluorescent protein (GFP) of jellyfish victoria is an unusual protein with strong visible fluorescence both in vivo and in vitro. In this study, about 750 bp fragment of gfp gene was amplified by PCR and was inserted into pRIT2T vector containing protein A gene. The recombinant plasmid was transferred into E. coli JM101. GFP and protein A were fused and expressed in E. coli Top10. The bright green fluorescence on the plates could be visible under UV at 365 nm. The bacteria cells were disrupted by sonic oscillation. The fused protein PA-GFP could be obtained from the supernatants.
Zhao
Zhijing
Z
Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China.
Liu
Xiumei
X
D004926:Escherichia coli / Q000235:genetics; D005453:Fluorescence; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics*; D011938:Rec A Recombinases / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics
D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D011938:Rec A Recombinases