[Application of cellulose acetate membrane with the technique of impression cytology combined with immunohistochemical staining for detection of dry eye].
OBJECTIVE
To evaluate the conjunctival cells by impression cytology with cellulose acetate membrane.


METHODS
The expression of the TGF-beta 1 in conjunctival epithelium of 24 patients with dry eye were studied by impression cytology combined with immunohistochemical staining and observed by microscope.


RESULTS
The cellulose acetate membrane is very transparent under the microscope. Cell's membrane by TGF-beta 1 staining is clear, cytoplasm brown and nuclei blue.


CONCLUSIONS
The method of impression cytology with cellulose acetate membrane is simple and has no injury to the ocular surface. Combined with immunohistochemistry staining, it is suitable to observe the conjunctival cells by this technique which has an important value for the examination of the ocular surface disease.
Lin
J
J
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Science, Guangzhou, 510060, China.
Luo
L
L
Zhang
P
P
Zhen
H
H
Feng
G
G
Zhong
X
X
D000368:Aged; D002482:Cellulose / Q000031:analogs & derivatives*; D003228:Conjunctiva / Q000378:metabolism / Q000473:pathology*; D015352:Dry Eye Syndromes / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics
D016212:Transforming Growth Factor beta; C005062:acetylcellulose; D002482:Cellulose