cDNA cloning and characterization of Agkistin, a new metalloproteinase from Agkistrodon halys.
Agkistin was a new snake venom metalloproteinase (SVMP) gene which was cloned from Agkistrodon halys. Its deduced amino acid sequence has two additional cysteines (Cys407 and Cys426) in the disintegrin domain compared to other RGD containing SVMPs. The full-length gene (Agkistin) and its disintegrin region (named Agkistin-s) were expressed by baculovirus expression system (pFastBac-Htb vector) with His-tag, and their platelet aggregation-inhibition activity was evaluated. The expressed protein Agkistin can also induce apoptosis of HMEC cells in the basal medium after incubated at 37 degrees C for 20 h.
Wang
Shao Hui
SH
State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, The Chinese Academy of Sciences, 320 Yue Yang Road, Shangai 200031, PR China.
Shen
Xiao Chuan
XC
Yang
Guang Zheng
GZ
Wu
Xiang Fu
XF
D017836:Agkistrodon / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001483:Base Sequence; D003001:Cloning, Molecular; D003435:Crotalid Venoms / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D008666:Metalloendopeptidases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010974:Platelet Aggregation / Q000502:physiology; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D012910:Snake Venoms / Q000201:enzymology*
D003435:Crotalid Venoms; D012910:Snake Venoms; D008666:Metalloendopeptidases; C472861:agkistin, Agkistrodon halys