Cloning, characterization and chromosomal localization of the Sus scrofa SLC31A1 gene.
Copper is an essential element necessary for normal function of numerous enzymes in all living organisms. Uptake of copper into the cell is thought to occur through the membrane protein, SLC31A1 (CTR1), which has been described in a variety of species including yeast, human and mouse. In this study, we present cloning, gene structure, chromosomal localization and expression pattern of the Sus scrofa SLC31A1 gene, which encodes a 189 amino acid protein. The (SSC) SLC31A1 gene is organized in four exons and spans an approximately 2.3 kb genomic region. We have localized the gene to chromosome 1q28-q2.13 using a somatic cell hybrid panel. This region shows conservation of synteny with human chromosome 9, where the human SLC31A1 (CTR1) gene has been localized. Expression studies suggest that SLC31A1 mRNA is transcribed in all tissues examined.
Harboe
T L
TL
Wilhelm Johannsen Centre for Functional Genome Research, Department of Medical Genetics, Institute of Medical Biochemistry, The Panum Institute, University of Copenhagen, Copenhagen, Denmark.
Jensen
L R
LR
Hansen
C
C
Horn
P
P
Bendixen
C
C
Tommerup
N
N
Tümer
Z
Z
D000818:Animals; D001483:Base Sequence; D027682:Cation Transport Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D000079923:Copper Transporter 1; D017931:DNA Primers; D040461:Gene Components; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics*
D027682:Cation Transport Proteins; D000079923:Copper Transporter 1; D017931:DNA Primers; C490079:SLC31A1 protein, human; C490077:Slc31a1 protein, mouse