Multilineage cells from adipose tissue as gene delivery vehicles.
We have characterized a population of mesenchymal progenitor cells from adipose tissue, termed processed lipoaspirate (PLA) cells, which have multilineage potential similar to bone marrow-derived mesenchymal stem cells and are also easily expanded in culture. The primary benefit of using adipose tissue as a source of multilineage progenitor cells is its relative abundance and ease of procurement. We examined the infection of PLA cells with adenoviral, oncoretroviral, and lentiviral vectors. We demonstrate that PLA cells can be transduced with lentiviral vectors at high efficiency. PLA cells maintain transgene expression after differentiation into adipogenic and osteogenic lineages after lentiviral transduction. Therefore, PLA cells and lentiviral vectors may be an efficient combination for use as a therapeutic gene delivery vehicle.
Morizono
Kouki
K
Microbiology, Immunology, and Molecular Genetics and Medicine, Department of Hematology-Oncology, UCLA AIDS Institute, UCLA School of Medicine, Los Angeles, CA 90024, USA.
De Ugarte
Daniel A
DA
Zhu
Min
M
Zuk
Pat
P
Elbarbary
Amir
A
Ashjian
Peter
P
Benhaim
Prosper
P
Chen
Irvin S Y
IS
Hedrick
Marc H
MH
D000256:Adenoviridae / Q000235:genetics; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage; D002478:Cells, Cultured; D016503:Drug Delivery Systems / Q000379:methods; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D015187:Lipectomy; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D013234:Stem Cells; D014161:Transduction, Genetic
D008164:Luminescent Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins