Cloning and molecular characterization of a novel gene strongly induced by the adenovirus E1A gene in rat thyroid cells.
Expression of the adenovirus E1A gene in the rat thyroid differentiated cell line PC Cl 3 induces thyrotropin-independent cell growth and impairs differentiation. However, the malignant phenotype is achieved only when the PC E1A cells are infected with other murine retroviruses carrying the v-abl, v-raf or polyoma middle-T genes. To determine through which genes E1A affects thyroid cells, we differentially screened PC Cl 3 and PC E1A cells. Here we report a new gene, named CL2, that is upregulated in PC E1A cells. The CL2 transcript is 4.4 kb long and encodes a 949 amino-acid protein. Conceptual translation of the open reading frame showed one product with a signal peptide, multiple nuclear localization signals and three newly described domains. Furthermore, in vivo, this protein was located juxtanuclear, which is suggestive of Golgian localization, and also in cytoplasm and nucleus/nucleolus. Finally, CL2 gene expression was drastically downregulated in human thyroid neoplastic cell lines and tissues.
Visconti
Roberta
R
Istituto di Endocrinologia ed Oncologia Sperimentale del CNR, via Pansini 5, 80131 Naples, Italy.
Schepis
Filippo
F
Iuliano
Rodolfo
R
Pierantoni
Giovanna Maria
GM
Zhang
Li
L
Carlomagno
Francesca
F
Battaglia
Caterina
C
Martelli
Maria Luisa
ML
Trapasso
Francesco
F
Santoro
Massimo
M
Fusco
Alfredo
A
D017500:Adenovirus E1A Proteins / Q000502:physiology*; D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D005796:Genes; D016258:Genes, mos; D016391:Genes, src; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D021382:Protein Sorting Signals; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000378:metabolism / Q000473:pathology; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured / Q000378:metabolism
D017500:Adenovirus E1A Proteins; D000952:Antigens, Polyomavirus Transforming; C471929:CL2 protein, rat; D009687:Nuclear Proteins; D021382:Protein Sorting Signals; D012333:RNA, Messenger