Purification and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major.
We report here the isolation of three isoforms of a novel C-terminally amidated peptide from the gills of red sea bream, Chrysophrys (Pagrus) major. Peptide sequences were determined by a combination of Edman degradation, MS and HPLC analysis of native and synthetic peptides. Three peptides, named chrysophsin-1, chrysophsin-2, and chrysophsin-3, consist of 25, 25, and 20 amino acids, respectively, and are highly cationic, containing an unusual C-terminal RRRH sequence. The alpha-helical structures of the three chrysophsin peptides were predicted from their secondary structures and were confirmed by CD spectroscopy. The synthetic peptides displayed broad-spectrum bactericidal activity against Gram-negative and Gram-positive bacteria including Escherichia coli, Bacillus subtilis, and fish and crustacean pathogens. The three peptides were also hemolytic. Immunohistochemical analysis showed that chrysophsins were localized in certain epithelial cells lining the surface of secondary lamellae and eosinophilic granule cell-like cells at the base of the secondary lamellae in red sea bream gills. Their broad ranging bactericidal activities, combined with their localization in certain cells and eosinophilic granule cell-like cells in the gills, suggest that chrysophsins play a significant role in the innate defense system of red sea bream gills.
Iijima
Noriaki
N
Laboratory of Molecular Cell Biology, Graduate School of Biosphere Science, Hiroshima University, Japan. noriiij@jpc.hiroshima-u.ac.jp
Tanimoto
Norio
N
Emoto
Yohko
Y
Morita
Yohko
Y
Uematsu
Kazumasa
K
Murakami
Tomoya
T
Nakai
Toshihiro
T
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D005880:Gills / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007124:Immunoenzyme Techniques; D054806:Magainins; D013058:Mass Spectrometry; D008555:Melitten / Q000494:pharmacology; D008969:Molecular Sequence Data; D011487:Protein Conformation; D020033:Protein Isoforms; D021381:Protein Transport; D021541:Sea Bream; D017386:Sequence Homology, Amino Acid; D029867:Xenopus Proteins; D014982:Xenopus laevis
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D054806:Magainins; D020033:Protein Isoforms; D029867:Xenopus Proteins; C472594:chrysophsin; C053301:magainin 2 peptide, Xenopus; D008555:Melitten