[Comparison of different lysis buffers in the assessment of the efficiency of single cell polymerase chain reaction for preimplantation genetic diagnosis].
OBJECTIVE
To set up a highly efficient cell lysis system to increase the efficiency of single cell polymerase chain reaction (PCR) for preimplantation genetic diagnosis (PGD).


METHODS
First we picked up one single lymphocyte or blastomere and lysed it in alkaline lysis buffer (ALB), proteinase K buffer (PK) and a new proteinase K lysis buffer compounded by ourselves. Then we amplified the 3rd exon of Gene ALD using nested PCR and compared the amplification efficiencies.


RESULTS
The amplification efficiencies of the single lymphocyte in ALB, PK and the lysis buffer compounded by ourselves were 90%, 74%, and 87%; those of the single blastomere were 98%, 77%, and 96%.


CONCLUSIONS
ALB and the lysis buffer compounded by ourselves are superior to PK in the lysis of cells in clinical PGD.
Li
Qi
Q
National Laboratory of Medical Genetics, Central South University, Changsha 410078, China.
Ma
Yan-lin
YL
Pan
Qian
Q
D000328:Adult; D005260:Female; D006801:Humans; D016133:Polymerase Chain Reaction; D019836:Preimplantation Diagnosis / Q000379:methods*