title: [The improvement on related condition of corneal cryopreservation and roles of cryopreserved corneas in emergent keratoplasty].
abstract: OBJECTIVE
  To study the cryopreserved corneal endothelium viability on the condition that a series of related factors on long-term corneal cryopreservation were modified. The frozen corneas were used for emergent penetrating keratoplasty on poor condition patients. To evaluate the role of cryopreserved corneas on clinical application, especially on emergent keratoplasty.
  
  
  METHODS
  Residual peripheral corneas were stained with Trypan blue and Alizarin red in order to assess endothelium survival rate after central grafts were punched. Specular microscope was used to measure corneal thickness and count endothelium density. The transparent rate of grafts, infective control rate, retinal restorative rate and the recovery degree of visual acurity were emphasized respectively.
  
  
  RESULTS
  Endothelium survival rate of grafts was 82.1% averagely. Postoperative endothelium density was 1,642 cells/mm2 and graft thickness was 0.59 mm averagely. The epithelial defects healed after 2-5 days of the operation and edematous grafts were reclear after 2-4 weeks of the operation in all grafts, except for chemical burn and thermal burn. The transparent rate was 87.5%. Infective controll rate was 90.63%. Retinal restorative rate was 55.56%. Some useful visual acurity can be obtained when the infection was under controlled and the retinal was reattached.
  
  
  CONCLUSIONS
  Modified long-term corneal cryopreservation is effective in maintaining endothelium viability and integrity. Furthermore, it could provide donors anytime because of it's unlimited preservative duration. Our study emphasizes especially the role of cryopreserved corneas in emergent keratoplasty.
authors.affiliation: Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
authors.forename: T
authors.initials: T
authors.lastname: Huang
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Lin
authors.forename: C
authors.initials: C
authors.lastname: Feng
mesh_terms: D000293:Adolescent; D000328:Adult; D000880:Anthraquinones; D003315:Cornea; D015925:Cryopreservation / Q000379:methods*; D004638:Emergency Treatment; D006085:Graft Survival; D006801:Humans; D016849:Keratitis, Herpetic / Q000601:surgery; D015948:Keratoplasty, Penetrating; D008297:Male; D009926:Organ Preservation; D013194:Staining and Labeling; D014343:Trypan Blue
chemical_list: D000880:Anthraquinones; C010078:alizarin; D014343:Trypan Blue