Vitreous cavity-associated immune deviation induced by retinal S antigen.
OBJECTIVE
To determine whether the vitreous cavity (VC) supports the induction of deviant immune responses to retinal soluble(S) antigen and to observe the influence of interleukin-1 (IL-1) on the immunologic properties of the VC.


METHODS
Retinal S antigen was inoculated into the anterior chamber(AC) and the VC in Wistar rats. Seven days after antigen inoculation, the recipient animals were immunized with S antigen and complete Freund's adjuvant. Delayed-type hypersensitivity (DTH) was assessed by footpad challenge. To alter systemic immune conditions, IL-1 was administrated by intraperitoneal injection.


RESULTS
Antigen-specific DTH did not develop in rats in which S antigen was injected into the AC and the VC. By contrast, when IL-1 administrated systemically, S antigen was injected into the AC and VC elicited strong DTH.


CONCLUSIONS
The VC supports immune deviation for soluble antigen by acitivity suppressing antigen-Specific DTH. Systemic administration of exogenous IL-1 eliminates the capacity of the VC to support immune deviation to soluble antigen locally injected.
Li
Z
Z
Department of Ophthalmology, Institute of Tissue Transplantation & Immunology Jinan University, Guangzhou 510632.
Peng
G
G
Li
C
C
D000818:Animals; D000867:Anterior Chamber / Q000276:immunology*; D000917:Antibody Formation; D019393:Arrestin / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007267:Injections; D007375:Interleukin-1 / Q000276:immunology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000276:immunology; D012995:Solubility; D014822:Vitreous Body / Q000276:immunology*
D019393:Arrestin; D007375:Interleukin-1