title: [Application of dot immunogold filtration assay for detecting serum antibodies in clonorchiasis patients].
abstract: OBJECTIVE
  To establish a new, fast, sensitive and specific assay for detecting antibodies in clonorchiasis.
  
  
  METHODS
  Using Clonorchis sinensis adult worm antigen and SPA labelled with colloidal gold as color developing agent, a dot immunogold filtration assay (DIGFA) for detecting clonorchiasis antibodies was tested. Dot-ELISA was used as the parallel control.
  
  
  RESULTS
  The positive rate of DIGFA and dot-ELISA in testing the sera from 119 proved cases were 96.4% (115/119) and 92.4% (110/119), respectively, the difference between the two assays being in significant (P > 0.05). The negative rate of DIGFA in healthy people was 100% (40/40). The cross reaction rate in 20 cysticercosis cases and 25 schistosomiasis cases were 5% (1/20) and 4% (1/25), respectively. Both coincidence rates comparing DIGFA with dot-ELISA were 90.9% (50/55).
  
  
  CONCLUSIONS
  DIGFA is as sensitive and specific as the dot-ELISA, and has the advantages of simplicity and without specific equipment.
authors.affiliation: Department of Parasitology, Guangxi Medical University, Nanning 530021.
authors.forename: D Y
authors.initials: DY
authors.lastname: Liu
authors.forename: W Q
authors.initials: WQ
authors.lastname: Hu
authors.forename: H M
authors.initials: HM
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D003003:Clonorchiasis / Q000175:diagnosis* / Q000276:immunology; D003004:Clonorchis sinensis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth