[6A8 cDNA encodes A type of alpha-mannosidase].
OBJECTIVE
To find the nature of the protein encoded by 6A8 cDNA.


METHODS
In the light of that the ligand of Con A is mannoses and that the function of alpha-mannosidase is trimming of mannoses in the oligosaccharide chains of glycoproteins, COS-7 cells and CNE-2L2 cells, a cloned cell line of human nasopharyngeal carcinoma, were transfected with pRc/CMV-sense 6A8 cDNA or pRc/CMV-antisense 6A8 cDNA and the Con A binding to the cells was examined upon a lazer scan confocal microscope.


RESULTS
The data demonstrated that with transfection of pRc/CMV-sense 6A8 cDNA the binding of Con A to the cells was decreased, but the staining of the cells with mAb 6A8 was enhanced, and vise versa for the cells with transfection of pRc/CMV-antisense 6A8 cDNA.


CONCLUSIONS
The protein encoded by 6A8 cDNA might be a type of alpha-mannosidase recognized by mAb 6A8.
Shi
G
G
Department of Immunology, Insitutute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Ma
F
F
Zhu
L
L
Zhang
L
L
Zhao
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D019556:COS Cells; D016373:DNA, Antisense / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics*; D006801:Humans; D019169:Jurkat Cells; D008253:Macaca mulatta; D008361:Mannosidases / Q000096:biosynthesis / Q000235:genetics*; D018613:Microscopy, Confocal; D009303:Nasopharyngeal Neoplasms / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured; D043323:alpha-Mannosidase
D000911:Antibodies, Monoclonal; D016373:DNA, Antisense; D018076:DNA, Complementary; D008361:Mannosidases; D043323:alpha-Mannosidase