[Analysis on the components of rat urinary stone induced by terephthalic acid].
In order to analyze the components of rat urinary stone and to explore the possible mechanism of chemically induced bladder cancer, terephthalic acid (TPA) is orally administrated to rats at the doses of 5000 and 500 mg/kg/BW/everyday for consecutive 90 days and stones in bladder and kidney are collected. Inductive Coupled Plasma Quantomer (ICP), Element Analyzer (EA) and Fourier Transform Infared Spectrometer (FT-IR) are applied for the analysis on the components of stone in bladder and kidney. The results showed that the main components of the stone are calcium, carbon, nitrogen, hydrogen and phosphorus. The FT-IR showed that the stones both in bladder and kidney might contain nitrous, carbonate, ammonium salt, and para-position replaced benzene-ring compounds, but no original TPA is detected. The findings indicate that TPA might have a metabolic turnover inside the rat body, and, at least, might not be completely excreted in its original form.
Qi
Shaoting
S
School of Public Health, Nanjing Medical University, Nanjing 210029, China.
Wang
Xinru
X
Xu
Xikun
X
Yao
Hongwei
H
D000818:Animals; D002118:Calcium / Q000032:analysis*; D002244:Carbon / Q000032:analysis; D010758:Phosphorus / Q000032:analysis*; D010795:Phthalic Acids; D051381:Rats; D017207:Rats, Sprague-Dawley; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D014545:Urinary Calculi / Q000139:chemically induced / Q000737:chemistry*
D010795:Phthalic Acids; D010758:Phosphorus; C011363:terephthalic acid; D002244:Carbon; D002118:Calcium