Tissue pharmacokinetics of florfenicol in pigs experimentally infected with Actinobacillus pleuropneumoniae.
The aim of this study has been to determine the tissue pharmacokinetic parameters of florfenicol in the pigs experimentally infected with Actinobacillus pleuropneumoniae. 21 crossed-bred (Duroc x Landrace x Yorkshire) local species of pigs were infected experimentally with Actinobacillus pleuropneumoniae serotype 1 and confirmed as typical sub-acute pleuropneumonia. A single dose of 20 mg/kg body weight of florfenicol, a novel animal-using antibiotic, was administrated intramuscularly in the pigs and then samples of blood, lung, trachea with bronchi, liver, kidney and muscle were taken at scheduled time points. Drug concentrations were determined by high performance liquid chromatography (HPLC) with an ultraviolet detector via extraction with ethyl acetate under nitrogen flow. The statistic moment theory (SMT) mathematic package was applied to calculate the tissue pharmacokinetic parameters of florfenicol in the infected model. AUC of lung, trachea with bronchi, liver, kidney and muscle were 121.69, 79.37, 81.05, 181.2, and 94.07 mg/l x h, respectively, MRT were from 34.66 to 90.17 h, and t1/2beta from 24.75 to 69.34 h, respectively.


CONCLUSIONS
Florfenicol was widely distributed in these tissues and maintained the effective therapeutic concentrations especially in the respiratory tract tissues that are the target organs of Actinobacillus pneuropneumoniae.


CONCLUSIONS
Tissue pharmacokinetic data could be evidence for regime designing of florfenicol in treatment of porcine pleuropneumonia.
Li
Jian-Zhong
JZ
Division of Pharmacology and Clinical Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, PR China.
Fung
Ki-Fai
KF
Chen
Zhang-Liu
ZL
Zeng
Zhen-Ling
ZL
Zhang
Jie
J
D000189:Actinobacillus Infections / Q000378:metabolism* / Q000382:microbiology; D016977:Actinobacillus pleuropneumoniae; D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D002701:Chloramphenicol / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D007273:Injections, Intramuscular; D015203:Reproducibility of Results; D013552:Swine; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D000900:Anti-Bacterial Agents; D002701:Chloramphenicol; C035534:florfenicol; D013839:Thiamphenicol