[Cloning and sequence analysis of LACK gene of Leishmania donovani isolates from plain and desert foci of China].
OBJECTIVE
To determine the nucleotide sequence of the LACK (Leishmania homologue of receptors for activated protein kinase C) gene of Leishmania donovani isolates from plain foci (L. d SD1) and desert foci (L. d XJ771) of China, and to find out the difference of the sequence of LACK gene with other Leishimania spp. and also the isolate from hill foci of China.


METHODS
The LACK genes of L. d SD1 and L. d XJ771 were amplified by RT-PCR and cloned into pUC18 vector respectively, sequenced by the dideoxy chain termination method, then analyzed and compared with that of other isolates.


RESULTS
The LACK genes of the two isolates were successfully cloned. Both of the 2 fragments were 942 bp in length. Comparison of the two nucleotide sequences with that of other Leishmania spp. in GenBank showed that the identities were more than 97%, and the identities of the nucleotide sequences of LACK genes of the three L. d isolates from plain, desert and hill foci of China were more than 95%.


CONCLUSIONS
High identities exist among the nucleotide sequences of LACK genes of the three L. d isolates from the three foci of China.
Ma
Ying
Y
Department of Parasitology, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610044.
Hu
Xiao-su
XS
Wang
Ya-jing
YJ
Wang
Zi-long
ZL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D007891:Leishmania / Q000235:genetics*; D008969:Molecular Sequence Data; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017385:Sequence Homology
D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface