[Study on the biological assay of Herba Leonuri--analysis the dosage response curve of Herba Leonuri and oxytocin and establishment of adequate potency pattern].
OBJECTIVE
To establish the effective patten of Herba Leonuri to be suitable for biological assay.


METHODS
By comparing the dosage-effect curve of Herba Leonuri and oxytocin the suitable effective pattern was set up.


RESULTS
The experiment suggest that the uterus contract height from 20 mm to 85 mm is the adequate effective range. In this range, the effect both Herba Leonuri and oxytocin should plot as a straight line against the log dose and two lines are basically parallel.


CONCLUSIONS
In the light of establishing effective pattern, the biological assay of Herba Leonuri may pattern the method of oxytocin biological assay to regard oxytocin as standard. The result error of Herba Leonuri assay is under 20%, not exceed the demand limits of biological assay.
Yang
Minghua
M
Zhejiang Provincial Institute of Chinese Medicine, Hangzhou 310023.
Yang
Subei
S
Jin
Zuhan
Z
Zhu
Shuangyou
S
D000818:Animals; D001681:Biological Assay; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D031332:Leonurus / Q000737:chemistry*; D010121:Oxytocin / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014590:Uterine Contraction / Q000187:drug effects*
D004365:Drugs, Chinese Herbal; D010121:Oxytocin