Thyroid hormones regulate the frequency of miniature end-plate currents in pre- and prometamorphic stages of the tadpole tail.
Thyroid hormones (THs), primarily 3,3',5-triiode-(L)-thyronine (T(3)), have been clearly established as natural inducers of apoptosis during metamorphosis of anuran embryos. We decided to use this phenomenon to test the hypothesis that, prior to genomic activation, T(3) has acute actions in the neuromuscular junction (NMJ) of the tail of amphibian embryos. We detected a dramatic increase in the production of miniature end-plate currents (MEPCs) 2-5 min after continuous application of T(3) (250 nM) using focal recordings under voltage clamp. Furthermore, this increase in the spontaneous release of neurotransmitter, evaluated by the MEPC frequency, was maintained for several hours. Reverse-T(3), the "inhibitory" form of THs, prevented this increase in MEPC frequency, suggesting that this is probably a highly specific action of T(3). In addition, the elevation in MEPC frequency induced by T(3) was unchanged in the presence or absence of extracellular calcium. The T(3)-mediated increase in MEPC frequency was blocked by niflumic acid, a nonsteroidal antinflammatory fenamate used to prevent the apoptotic volume decrease observed in many systems. The present study demonstrated that T(3) induces a remarkable nongenomic action in the NMJ of the tadpole tail at pre- and promatamorphic stages.
Rojas
Legier V
LV
Department of Physiology, School of Medicine, Universidad Central del Caribe, and Center for Molecular and Behavioral Neuroscience, Bayamón, Puerto Rico. lrojas@uccaribe.edu
Bonilla
Laurie
L
Baez
Sheila
S
Lasalde-Dominicci
José A
JA
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D018118:Chloride Channels / Q000037:antagonists & inhibitors; D005110:Extracellular Space / Q000378:metabolism; D066298:In Vitro Techniques; D007814:Larva; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008675:Metamorphosis, Biological / Q000502:physiology*; D009469:Neuromuscular Junction / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D018377:Neurotransmitter Agents / Q000378:metabolism; D009544:Niflumic Acid / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D011892:Rana catesbeiana; D013623:Tail / Q000187:drug effects / Q000294:innervation* / Q000502:physiology; D014284:Triiodothyronine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D014285:Triiodothyronine, Reverse / Q000494:pharmacology*
D018118:Chloride Channels; D018377:Neurotransmitter Agents; D014284:Triiodothyronine; D009544:Niflumic Acid; D014285:Triiodothyronine, Reverse; D000109:Acetylcholine; D002118:Calcium