title: [Study on metabolites on aconitine in rabbit urine].
abstract: OBJECTIVE
  To identify the main metabolites of aconitine in the urine of rabbits.
  
  
  METHODS
  After oral administration of aconitine (5 mg.kg-1), the urine of male rabbits was collected and extracted by solid phase extraction and analyzed by liquid chromatography-ion trap mass spectrometry.
  
  
  RESULTS
  Aconitine and 4 metabolites were found in the rabbit urine. Their protonated molecular ions at m/z 632, m/z 604, m/z 590, m/z 500 and multistage fragment ions with neutral loss of 60 u, 32 u, 28 u and 18 u were monitored. Their relative concentration were M1 > Aconitine > M4 > M2 > M3.
  
  
  CONCLUSIONS
  The metabolites M1-M4 were deduced as 16-O-demethylaconitine, benzoylaconine, 16-O-demethylbenzoylaconine and aconine, respectively.
authors.affiliation: Department of Natural Pharmaceutical Chemistry, School of Pharmacy, jilin University, Changchun 130021, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Hong-gui
authors.initials: HG
authors.lastname: Zhang
authors.forename: Xiang-guo
authors.initials: XG
authors.lastname: Shi
authors.forename: Ming-yu
authors.initials: MY
authors.lastname: Duan
authors.forename: Da-fang
authors.initials: DF
authors.lastname: Zhong
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine*; D000470:Alkaloids / Q000652:urine; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008297:Male; D011817:Rabbits; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: C486136:16-O-demethylaconitine; D000470:Alkaloids; C047307:benzoylaconine; C047306:aconine; D000157:Aconitine