A dorso-ventral gradient of Pax6 in the developing retina suggests a role in topographic map formation.
Expression of the transcription factor Pax6 was assessed immunohistochemically in embryonic chick retina during retino-tectal map formation. A low dorsal to high ventral gradient was found that correlated with expression of the axonal guidance cue EphB2. Furthermore, transfection of Pax6 into undifferentiated P19 cells up-regulated EphB2. The results raise the possibility that Pax6 is upstream of EphB2 and that its graded expression defines the dorso-ventral axis of the retino-tectal projection.
Ziman
Melanie
M
Department of Biomedical Science, Edith Cowan University, Western Australia, 6027, Nedlands, Australia.
Rodger
Jennifer
J
Lukehurst
Sherralee
S
Hancock
Davina
D
Dunlop
Sarah
S
Beazley
Lyn
L
D000818:Animals; D001369:Axons / Q000502:physiology; D002460:Cell Line; D002642:Chick Embryo; D004622:Embryo, Mammalian / Q000378:metabolism; D005314:Embryonic and Fetal Development; D005136:Eye Proteins; D018398:Homeodomain Proteins / Q000378:metabolism* / Q000502:physiology; D007150:Immunohistochemistry; D051379:Mice; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D036183:Receptor, EphB2 / Q000378:metabolism; D012097:Repressor Proteins; D012160:Retina / Q000196:embryology*; D013477:Superior Colliculi / Q000196:embryology; D014162:Transfection; D015854:Up-Regulation
D005136:Eye Proteins; D018398:Homeodomain Proteins; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; C000605732:Pax6 protein, mouse; D012097:Repressor Proteins; D036183:Receptor, EphB2