[A chemiluminescence flow sensor for vitamin B1].
OBJECTIVE
To determine vitamin B1 in pharmaceutical preparations by chemiluminescence (CL) flow sensor.


METHODS
When 200 microL of Na3PO4 was passed through an anion exchange column, K3Fe(CN)6 was eluted from the resin and then mixed with the vitamin B1 stream containing NaOH to product CL, by the fast oxidation reaction between vitamin B1 and K3Fe(CN)6 in alkaline solution.


RESULTS
The CL emission intensity was correlated with the vitamin B1 concentration in the range 1.0 x 10(-5) to 1.0 x 10(-3) mol.L-1 and the detection limit was 8.0 x 10(-6) mol.L-1 (3 sigma). A complete analysis, including sampling and washing, can be performed within 2 min with a relative standard deviation of less than 4.0%.


CONCLUSIONS
The vitamin B1 flow sensor was stable for over 200 analyses and has been applied successfully to determination of vitamin B1 in pharmaceutical preparations.
Song
Z H
ZH
Department of Chemistry, Northwest University, Xi'an 710069, China. songzhenghua@hotmail.com
Huang
J X
JX
Wang
R
R
D007267:Injections; D008163:Luminescent Measurements; D013607:Tablets / Q000737:chemistry; D013831:Thiamine / Q000032:analysis*
D013607:Tablets; D013831:Thiamine