[Taxonomic studies on Echinochasmus fujienensis and its related species by random amplified polymorphic DNA analysis and experimental infection].
OBJECTIVE
To explore the identification and differentiation of Echinochasmus fujianensis, Echinochasmus japonicus(Jiangxi strain and Fujian strain), Echinochasmus liliputanus Anhui and Echinochasmus per foliatus Hubei.


METHODS
Random amplified polymorphic DNA analysis (RAPD) and experimental animal infection were performed.


RESULTS
469 polymorphic DNA fragments were obtained by 28 primers from 4 Echinochasmus species and strains in Fujian, Anhui and Jiangxi. 20.8% and 97.6% of the fragments in Echinochasmus fujianensis were the same as those in Echinochasmus japonicus Fujian strain and in Echinochasmus liliputanus Anhui, respectively, 99.7% of the fragments were the same between Echinochasmus japonicus Jiangxi strain and Fujian strain.


CONCLUSIONS
Echinochasmus fujianensis and Echinochasmus liliputanus Anhui are the same species. Echinochasmus fujienensis is an independent species different from Echinochasmus japonicus. Polyinfection of Echinochasmus fujianensis, Echinochasmus japonicus and Echinochasmus perfoliatus exist in all the 3 provinces, Hubei, Anbui and Fujian, of which Echinochasmus fujienensis is a dominant species.
Cheng
Y
Y
Fujian Provincial Institute of Parasitic Discases, Fuzhou 350001.
Zhang
Y
Y
Lin
C
C
Zhan
Z
Z
Yu
X
X
Lin
J
J
Cai
M
M
Che
W
W
D000818:Animals; D018509:DNA, Helminth / Q000032:analysis; D005399:Fishes / Q000469:parasitology; D006374:Helminthiasis, Animal / Q000469:parasitology; D019105:Random Amplified Polymorphic DNA Technique; D012908:Snails / Q000469:parasitology; D013045:Species Specificity; D014200:Trematoda / Q000145:classification* / Q000235:genetics
D018509:DNA, Helminth