[The anticancer effect of artesunate and its mechanism].
OBJECTIVE
To study the anticancer effect of artesunate and its mechanism.


METHODS
To observe the effect of artesunate on the growth of solid tumor and the expression of PCNA, Bcl-2 and Bax genes in mice bearing H22 solid hepatic carcinoma.


RESULTS
After administration of artesunate (ig, 300 mg.kg-1.d-1 x 7 d), growth of solid tumor was obviously inhibited, the tumor inhibitory rates were 49.1%, 48.7% and 46.6% and 46.6% in 3 experiments. The apoptosis of liver cancer cells were increased. Immunohistochemical staining showed that the number of Bcl-2 protein positive cells were decreased, but the number of Bax protein positive cells were increased. The PCNA positive cells were significantly lower than those in the control group.


CONCLUSIONS
Artesunate showed obvious anticancer activity on H22 hepatic carcinoma bearing mice and undergo apoptosis of liver cancer cells. The mechanism of anticancer effect of artesunate may be related to down-regulation of the expression of PCNA and Bcl-2 genes and up-regulation of the expression of Bax gene.
Wang
Qin
Q
Unit of Chinese Traditional and Herbal Drugs, First Affiliated Hospital, Guangxi Traditional Chinese Medical University, Nanning 530001, China. wq@gxtcmu.edu.cn
Wu
Li-mao
LM
Zhao
Yi
Y
Zhang
Xi-liu
XL
Wang
Nai-ping
NP
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use*; D017209:Apoptosis; D037621:Artemisinins / Q000627:therapeutic use*; D000077332:Artesunate; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D011897:Random Allocation; D012717:Sesquiterpenes / Q000627:therapeutic use*; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays; D051028:bcl-2-Associated X Protein
D000972:Antineoplastic Agents, Phytogenic; D037621:Artemisinins; C494827:Bax protein, mouse; D018809:Proliferating Cell Nuclear Antigen; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012717:Sesquiterpenes; D051028:bcl-2-Associated X Protein; D000077332:Artesunate