Semliki Forest virus vectors for rapid and high-level expression of integral membrane proteins.
Semliki Forest virus (SFV) vectors have been applied for the expression of recombinant integral membrane proteins in a wide range of mammalian host cells. More than 50 G protein-coupled receptors (GPCRs), several ion channels and other types of transmembrane or membrane-associated proteins have been expressed at high levels. The establishment of large-scale SFV technology has facilitated the production of large quantities of recombinant receptors, which have then been subjected to drug screening programs and structure-function studies on purified receptors. The recent Membrane Protein Network (MePNet) structural genomics initiative, where 100 GPCRs are overexpressed from SFV vectors, will further provide new methods and technologies for expression, solubilization, purification and crystallization of GPCRs.
Lundstrom
Kenneth
K
Bio-Xtal, Chemin de Croisettes 22, CH-1066 Epalinges, Switzerland. kenneth.lundstrom@mepnet.org
D000818:Animals; D019149:Bioreactors; D003460:Crystallization; D005822:Genetic Vectors; D006801:Humans; D007473:Ion Channels / Q000096:biosynthesis / Q000737:chemistry; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D043562:Receptors, G-Protein-Coupled; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D012672:Semliki forest virus
C109334:GPR1 protein, S cerevisiae; D007473:Ion Channels; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D029701:Saccharomyces cerevisiae Proteins