Plasmodium immobilization of Physarella oblonga (Berk. & Curt.) Morgan (Myxomycetes) using kaolinite as a matrix of entrapment.
The possibility of using kaolinite-immobilized plasmodium fragments of Physarella oblonga (Berk. & Curt.) Morgan to maintain their metabolic activity was examined. The immobilization process was carried out with 1 mg of plasmodium of P. oblonga entrapped in 10 g of kaolinite. Sodium acetate (1 mM) was used as a metabolic precursor. The collection of fractions was carried out during a one month period, and extracted with ether/ethyl acetate and chloroform/acetonitrile. The extractions from plasmodium in natura were accomplished with the same solvents. The extracts obtained were analyzed in a spectrophotometer at 266 nm and 310 nm, and by thin layer chromatography to assess the productivity of the immobilized plasmodium. The absorbances of the extracts in both wavelengths and the chromatographic tests showed the synthesis of compounds by the immobilized material. Three chromatographic spots were observed in the extracts obtained from the immobilized plasmodium. Two spots coincided with the R(f) values and coloration of the spots observed for the material in natura used as a reference. The kaolinite-immobilized plasmodium of P. oblonga can remain metabolically active for at least one month at room temperature and ambient light conditions.
Ribeiro
Sheyla M
SM
Laboratório de Myxomycetes, Departamento de Botânica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego s/n, CEP 50670-901, Recife, PE, Brazil. sheylaribeiro@hotmail.com
Cavalcanti
Laise H
LH
Pereira
Eugênia C
EC
Silva
Nicácio H
NH
D007616:Kaolin / Q000494:pharmacology*; D009235:Myxomycetes / Q000737:chemistry / Q000378:metabolism*; D019346:Sodium Acetate / Q000378:metabolism
D007616:Kaolin; D019346:Sodium Acetate