[Studies on immune responses in mice to expressed products from CSP gene of Plasmodium falciparum FCC1/HN isolate].
OBJECTIVE
To construct the eukaryotic expression system with pcDNA3-PfCSP/HeLa for CSP gene of Plasmodium falciparum, and to observe the immune responses in BALB/c mice induced by the expressed proteins.


METHODS
The recombinant plasmid pcDNA3-PfCSP was transformed into mammalian cell line of human HeLa cells. The expressed protein was isolated and analyzed by SDS-PAGE and used for immunization of BALB/c mice by subcutaneous, intravenous or intraperitoneal administration, respectively. ELISA, Western blotting, T lymphocyte proliferation test, natural killer cell activity and CD4+ and CD8+ T cells detection were used for observation of the level of humoral and cellular immune responses.


RESULTS
Immune sera strongly reacted with the expressed protein. The titer of the antibodies was up to 1:6,400 by ELISA. Western blotting analysis revealed a specific band at 38.3 kDa. The specific proliferative response with immunized BALB/c mice spleen cells was remarkablely higher than that with control ones. The ratio of CD4+ and CD8+ T cells and the NK cell activity were significantly higher in the immunization group than that in the control groups.


CONCLUSIONS
The humoral and cell-mediated immune responses and elevated NKC activity to expressed products with the eukaryotic expression system can specifically be detected in BALB/c mice to generate, indicating that the expressed protein could enhance immune activity in mice.
Fang
Z
Z
Institute of Parasitology, Sun Yat-Sen University of Medical Sciences, Guangzhou 510089.
Yu
X
X
Liu
Y
Y
Luo
S
S
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D017125:Genes, Protozoan; D006367:HeLa Cells; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010963:Plasmodium falciparum / Q000235:genetics / Q000276:immunology*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*
D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan