title: Gonadotropin-releasing hormone-induced stimulation of the rat secretogranin II promoter involves activation of CREB.
abstract: To investigate the events involved in regulation of the secretogranin II (SgII) gene, luciferase reporter constructs were transfected into gonadotrope-derived, alphaT3-1 cells. DNA between -91 and -60 relative to the transcription start site was found to be required for GnRH induced SgII reporter gene activation. This region contains a consensus cAMP response element (CRE) and disruption of this CRE reduced GnRH responsiveness of the SgII promoter. CREB was shown to bind to the SgII CRE and transfection studies with a dominant-negative CREB mutant provided evidence that CREB is required for GnRH responsiveness of the SgII promoter. An expression vector for an inhibitor of the cAMP-dependent protein kinase was found to reduce the ability of cAMP or GnRH to activate the SgII-luciferase reporter gene. These studies offer evidence that GnRH-induced activation of the SgII promoter in the alphaT3-1 cell line requires cAMP-dependent protein kinase activity and a functional CRE within the 5'-flanking region of the gene.
authors.affiliation: Department of Biochemistry, Institute of Biotechnology, Chungnam National University, Taejon 305-764, South Korea.
authors.forename: Seok Bean
authors.initials: SB
authors.lastname: Song
authors.forename: Myungchull
authors.initials: M
authors.lastname: Rhee
authors.forename: Mark S
authors.initials: MS
authors.lastname: Roberson
authors.forename: Richard A
authors.initials: RA
authors.lastname: Maurer
authors.forename: Kyoon Eon
authors.initials: KE
authors.lastname: Kim
mesh_terms: D024506:5' Flanking Region; D000818:Animals; D002460:Cell Line; D002864:Chromogranins; D000242:Cyclic AMP / Q000494:pharmacology; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D051379:Mice; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D011506:Proteins / Q000235:genetics*; D051381:Rats; D020218:Response Elements; D015533:Transcriptional Activation; D014162:Transfection
chemical_list: D002864:Chromogranins; D017362:Cyclic AMP Response Element-Binding Protein; D011506:Proteins; D007987:Gonadotropin-Releasing Hormone; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases