Use of the confocal laser scanning microscope in studies on the developmental biology of marine crustaceans.
Confocal Laser Scanning Microscope techniques have been applied to study the developmental biology of marine copepods and decapod larvae. The lipophylic probes DiI and DiOC(6) were used to study both the external and internal morphology of these crustaceans, whereas the same DiOC(6) and the specific nuclear probe Hoechst 33342 were used to study embryonic development of copepods in vivo. To distinguish viable from non-viable copepod embryos, the vital dye dichlorodihydrofluorescein diacetate (H(2)DCFDA) was used. Major advantages and difficulties in the use of these non-invasive techniques in studies of the reproductive biology of marine crustaceans are discussed.
Buttino
Isabella
I
Stazione Zoologica "Anton Dohrn," 80122 Napoli, Italy. buttino@alpha.szn.it
Ianora
Adrianna
A
Carotenuto
Ylenia
Y
Zupo
Valerio
V
Miralto
Antonio
A
D000818:Animals; D001562:Benzimidazoles / Q000378:metabolism; D002232:Carbocyanines / Q000378:metabolism; D033342:Copepoda / Q000196:embryology* / Q000648:ultrastructure; D033364:Decapoda / Q000196:embryology* / Q000648:ultrastructure; D004625:Embryo, Nonmammalian / Q000648:ultrastructure*; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism; D007814:Larva / Q000648:ultrastructure; D018613:Microscopy, Confocal / Q000379:methods; D012623:Seawater
C035005:3,3'-dihexadecylindocarbocyanine; D001562:Benzimidazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; C007392:3,3'-dihexyl-2,2'-oxacarbocyanine; C017807:bisbenzimide ethoxide trihydrochloride