[Cloning and sequencing of fragments associated with cytoplasm male sterility of rice].
Mitochorndrial DNA (mtDNA), nuclear DNA (nDNA), and genomic DNA (gDNA) were individually extracted from Zhenshan 97A, Xieqingzao A and A-23, which were cytoplasmic male sterile (wild abortive, dwarf abortive and BT), and from Zhenshan 97B, Xieqingzao B and BT, which were maintainers lines. Each of them was analyzed with 500 random primers by RAPD. Six fragments specific to male sterile lines were amplified. A fragment, PWH-17, associated with wild and dwarf abortive type cytoplasmic male sterility was analyzed by Southern blotting and sequencing, and also tested by SCAR. It was 1879 bp in length, which contained 6 open reading frames and 8 repeated sequences. BLAST search revealed that partial sequence in PWH-17 showed high similarity to the upstream sequence of tRNA-Asp gene in Elytrigia elongate (GenBank accession number U14335) and wheat (X13243). The similarity ratios were 97% and 100%. It was inferred that the possible position of PWH-17 in rice (wild abortive) could be located between tRNA-Asp and Cox II, which will be discussed further.
Yang
Jian-Bo
JB
Key Lab of Rice Genetics and Breeding, Ministry of Agriculture, Anhui Academy of Agricultural Sciences, Hefei 230031, China. yjianbo@mail.hf.ah.cn
Wang
Xiu-Feng
XF
Zhao
Cheng-Song
CS
Xiang
Tai-He
TH
Li
Li
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics*; D005298:Fertility / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004272:DNA, Mitochondrial; D018744:DNA, Plant