A simplified method for identification of human cardiac myosin heavy-chain isoforms.
Cardiac myosin is a central participant in the cross-bridge cycling that mediates myocyte contraction and consists of multiple subunits that mediate both hydrolysis of ATP and mechanical production of contractile force Two isoforms of myosin heavy chain (MHC- alpha and MHC- beta ) are known to exist in mammalian cardiac tissue, and it is within this myosin subunit that ATPase activity resides. These isoforms differ by less than 0.2% in total molecular mass and amino acid sequence, but, strikingly, influence the rate and efficiency of energy utilization for generation of contractile force. Changes in the MHC- alpha /MHC- beta ratio has been classically viewed as an adaptation of a failing myocyte in both animal models and humans; however, their measurement has traditionally required specialized preparations and materials for sufficient resolution. Here we describe a greatly simplified method for routine assessments of myosin isoform composition in human cardiac tissues. The primary advantages of our approach include higher throughput and reduced supply costs with no apparent loss of statistical power, reproducibility or achieved results. Use of this more convenient method may provide enhanced access to an otherwise specialized technique and could provide additional opportunity for investigation of cardiac myocyte adaptive changes.
Piao
Shengfu
S
Division of Pharmacology, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.
Yu
Fushun
F
Mihm
Michael J
MJ
Reiser
Peter J
PJ
McCarthy
Patrick M
PM
Van Wagoner
David R
DR
Bauer
John Anthony
JA
D024722:Cardiac Myosins / Q000032:analysis* / Q000145:classification / Q000378:metabolism*; D046508:Culture Techniques; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D006325:Heart Atria / Q000378:metabolism; D006801:Humans; D018995:Myosin Heavy Chains / Q000032:analysis* / Q000145:classification / Q000378:metabolism*; D020033:Protein Isoforms / Q000032:analysis / Q000145:classification / Q000378:metabolism
D020033:Protein Isoforms; D024722:Cardiac Myosins; D018995:Myosin Heavy Chains