[Effect of ligustrazine on bone marrow hematopoiesis in mice after bone marrow transplantation].
OBJECTIVE
To investigate the effect of ligustrazine (LT) on hematopoiesis in mice after bone marrow isotransplantation (iso-BMT).


METHODS
The typical model of iso-BMT was established and the model mice were randomly divided into two groups, the LT group treated with LT injection 0.2 ml and the control group treated with normal saline 0.2 ml, twice a day by gastrogavage. The following parameters were observed in the day 1, 7 and 14: peripheral blood cells, bone marrow mono-nuclear cells (BMMNC), heparin sulfate (HS) expression in bone marrow section by immunohistochemical SABC-AP method, stromal cell derived factor-1 (SDF-1) expression and CXC chemotaxis factor receptor 4 (CXCR4) expression.


RESULTS
The levels of peripheral WBC, platelet, BMMNC, CXCR4, HS, SDF-1 at the day 7 and 14 in the LT group were all higher significantly than those in the control group (P < 0.05 or P < 0.01).


CONCLUSIONS
LT could improve the bone marrow hematopoiesis in the early hematopoietic re-establishing stage after BMT.
Sun
Han-ying
HY
Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430030.
Fang
Ming-hao
MH
Ren
Tian-hua
TH
D000818:Animals; D016026:Bone Marrow Transplantation; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects*; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011719:Pyrazines / Q000494:pharmacology*; D011897:Random Allocation; D019718:Receptors, CXCR4 / Q000097:blood
D011719:Pyrazines; D019718:Receptors, CXCR4; C017953:tetramethylpyrazine