Affinity hemodialysis for antiviral therapy. II. Removal of HIV-1 viral proteins from cell culture supernatants and whole blood.
BACKGROUND
HIV-1 gp120 may play a role in the progression from HIV infection to AIDS.


OBJECTIVE
We investigated affinity hemodialysis for removing gp120 from cell culture and whole blood.


METHODS
Anti-gp120 antibodies covalently coupled to agarose beads were packed into columns or hollow-fiber hemodialysis cartridges. Supernatants from HIV-infected HL2/3 cells or gp120 containing whole blood were pumped over the columns and gp120 measured by ELISA.


RESULTS
Anti-gp120 agarose removed approximately 90% of HIV-1 gp120 from HL2/3 cultures in 30-60 min. Capture was antibody-dependent (F105 > IDX 1121 > ABI 13-108). Affinity hemodialysis also efficiently captured gp120 from buffer in a first-order, flow-rate-dependent fashion (t((1/2)) = 13 min at 0.9 ml/min). Clearance was faster than calculated diffusion (t((1/2)) approximately 2.5 h) suggesting significant convective transport. gp120 removal from blood was slower (t((1/2)) = 1.4 h).


CONCLUSIONS
Affinity hemodialysis efficiently clears gp120 from cell culture fluids and blood and may be useful in slowing the progression to AIDS.
Tullis
Richard H
RH
Aethlon Medical, Inc., San Diego, Calif 92121, USA. rhtullis@aethlonmedical.com
Duffin
R Paul
RP
Zech
Marvin
M
Ambrus
Julian L
JL
D001769:Blood / Q000821:virology; D002846:Chromatography, Affinity; D017077:Culture Media, Conditioned; D015699:HIV Envelope Protein gp120 / Q000097:blood / Q000302:isolation & purification; D015658:HIV Infections / Q000097:blood / Q000628:therapy*; D015497:HIV-1; D006801:Humans; D007700:Kinetics; D006435:Renal Dialysis / Q000379:methods* / Q000592:standards
D017077:Culture Media, Conditioned; D015699:HIV Envelope Protein gp120