title: Actomyosin motility on nanostructured surfaces.
abstract: We have here, for the first time, used nanofabrication techniques to reproduce aspects of the ordered actomyosin arrangement in a muscle cell. The adsorption of functional heavy meromyosin (HMM) to five different resist polymers was first assessed. One group of resists (MRL-6000.1XP and ZEP-520) consistently exhibited high quality motility of actin filaments after incubation with HMM. A second group (PMMA-200, PMMA-950, and MRI-9030) generally gave low quality of motility with only few smoothly moving filaments. Based on these findings electron beam lithography was applied to a bi-layer resist system with PMMA-950 on top of MRL-6000.1XP. Grooves (100-200nm wide) in the PMMA layer were created to expose the MRL-6000.1XP surface for adsorption of HMM and guidance of actin filament motility. This guidance was quite efficient allowing no U-turns of the filaments and approximately 20 times higher density of moving filaments in the grooves than on the surrounding PMMA.
authors.affiliation: Division of Solid State Physics and The Nanometer Consortium, University of Lund, Sweden.
authors.forename: Richard
authors.initials: R
authors.lastname: Bunk
authors.forename: Jeanna
authors.initials: J
authors.lastname: Klinth
authors.forename: Lars
authors.initials: L
authors.lastname: Montelius
authors.forename: Ian A
authors.initials: IA
authors.lastname: Nicholls
authors.forename: Pär
authors.initials: P
authors.lastname: Omling
authors.forename: Sven
authors.initials: S
authors.lastname: Tågerud
authors.forename: Alf
authors.initials: A
authors.lastname: Månsson
mesh_terms: D008841:Actin Cytoskeleton / Q000502:physiology; D000205:Actomyosin / Q000502:physiology* / Q000648:ultrastructure; D000327:Adsorption; D004581:Electronics; D018625:Microscopy, Atomic Force; D009068:Movement; D036103:Nanotechnology; D011108:Polymers / Q000737:chemistry
chemical_list: D011108:Polymers; D000205:Actomyosin