Adiponutrin mRNA expression in white adipose tissue is rapidly induced by meal-feeding a high-sucrose diet.
Adiponutrin is a recently identified gene of unknown function that is expressed exclusively in adipose tissues. To provide information about its physiological regulation and possible function, the effect of meal-feeding rats on the expression of adiponutrin mRNA in white adipose tissue was studied. A high-sucrose meal increased adiponutrin mRNA levels by at least 5-fold within 3 h. Post-meal levels returned to pre-meal levels with a half-life of about 5 h. The induction was prevented by injection of actinomycin-D prior to the meal. This pattern of expression was very similar to that seen for leptin mRNA. There were only minimal, or no, effects on acrp30/adiponectin, resistin, adipsin, or stearoyl-CoA desaturase 1. Adiponutrin appears more like leptin with respect to its acute regulation by meal-feeding than to any of the other adipokines or to enzymes directly involved in lipogenesis. This suggests that adiponutrin could be involved in overall energy homeostasis, as is leptin.
Polson
Dagmar A
DA
Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand.
Thompson
Mary P
MP
D052242:Adiponectin; D000273:Adipose Tissue / Q000502:physiology*; D000818:Animals; D018797:Cell Cycle Proteins; D011416:Complement Factor D; D003609:Dactinomycin / Q000378:metabolism; D019422:Dietary Sucrose / Q000008:administration & dosage*; D006706:Homeostasis; D006729:Hormones, Ectopic / Q000235:genetics / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D020738:Leptin / Q000235:genetics / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D020932:Nerve Growth Factor; D019384:Nucleic Acid Synthesis Inhibitors / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D052243:Resistin; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D052242:Adiponectin; D018797:Cell Cycle Proteins; D019422:Dietary Sucrose; D006729:Hormones, Ectopic; D036341:Intercellular Signaling Peptides and Proteins; D020738:Leptin; D008565:Membrane Proteins; D019384:Nucleic Acid Synthesis Inhibitors; D011506:Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D052243:Resistin; C499622:Retn protein, rat; C486575:Retnla protein, rat; C436316:adiponutrin; D003609:Dactinomycin; D020932:Nerve Growth Factor; C490333:Scd1 protein, mouse; D013230:Stearoyl-CoA Desaturase; D012697:Serine Endopeptidases; D011416:Complement Factor D; C493491:complement factor D, mouse