title: [Preliminary study on the levels of natural antibodies against Schistosoma japonicum in Microtus fortis in Dongting lake area].
abstract: OBJECTIVE
  To explore the detection rate and the levels of natural antibodies to cercarial antigen(CA), juvenile antigen(JA), adult worm antigen (AWA) and soluble egg antigen (SEA), respectively, derived from Schistosoma japoncum in Microtus fortis distributed in Dongting Lake area.
  
  
  METHODS
  The antibodies in wild Microtus fortis(WMF), laboratory-bred Microtus fortis (BMF) and laboratory-bred Microtus fortis 15 days after S. japonicum infection (BMFi15) were detected by indirect ELISA and a comparison of the antibody levels between Microtus fortis including WMF, BMF, BMFi15 and albino mice without infection (AM), 15 days after infection(AMi15) and 42 days after infection (AMi42) was made.
  
  
  RESULTS
  The positive rates of detection for anti-JA-Ab in WMF and BMF were 94.6% and 94.4%, respectively, followed by 85.5% and 83.3% for anti-AWA-Ab, 58.1% and 59.4% for anti-SEA-Ab, and 14.3% and 13.9% for anti-CA-Ab. The levels of antibodies to the above four antigens in BMFi15 increased significantly, the detection rates being 100% for anti-JA-Ab and anti-AWA-Ab, 84.4% for anti-SEA-Ab and 65.6% for anti-CA-Ab. The levels of antibodies in WME, BMF and BMFi15 had the similar trend(JA > AWA > SEA > CA), and those in BMFi15 to CA, JA, AWA and SEA increased by 1.9, 2.2, 2.1 and 1.5 folds, respectively, when compared with BMF.
  
  
  CONCLUSIONS
  Natural antibodies to Schistosoma japonicum were found in wild and labortorybred Microtus fortis. The levels of antibodies in BMFi15 to four above antigens were apparently higher than those in AMi15.
authors.affiliation: Hunan Provincal Institute of Parasitic Diseases, Yueyang 414000.
authors.forename: Y
authors.initials: Y
authors.lastname: He
authors.forename: X
authors.initials: X
authors.lastname: Luo
authors.forename: X
authors.initials: X
authors.lastname: Yu
authors.forename: J
authors.initials: J
authors.lastname: Lin
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology; D003411:Arvicolinae / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007113:Immunity, Innate; D051379:Mice; D012549:Schistosoma japonicum / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth