Acteoside from Cistanche salsa inhibits apoptosis by 1-methyl-4-phenylpyridinium ion in cerebellar granule neurons.
In this study we assessed the effect of acteoside that significantly improved cell viability and inhibited lactate dehydrogenase (LDH) release. Furthermore acteoside prevented a neurotoxin 1-methyl-4-phenylpyridinium ion (MPP+)-induced apoptosis in CGNs. Accordingly, our flow cytometric analysis of CGNs after acteoside treatment revealed a decrease in the number of the MPP+-induced apoptotic cells (P < 0.001). Western blot analysis demonstrated that acteoside inhibits the active caspase-3 fragment (17 kDa) (P < 0.001) and the proteolytic poly (ADP-ribose) polymerase (PARP) fragment (85 kDa) expression (P < 0.001) following MPP + treatment in CGNs. We conclude that acteoside prevents the MPP+-induced apoptosis and inhibits the apoptosis-related pathway.
Pu
Xiaoping
X
Song
Zhihong
Z
Li
Yanyun
Y
Tu
Pengfei
P
Li
Huinong
H
D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002531:Cerebellum / Q000166:cytology; D002681:China; D031671:Cistanche / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D059808:Polyphenols
D015655:1-Methyl-4-phenylpyridinium; D005960:Glucosides; D010636:Phenols; C058956:acteoside; D059808:Polyphenols