title: [6A8 cDNA encodes A type of alpha-mannosidase].
abstract: OBJECTIVE
  To find the nature of the protein encoded by 6A8 cDNA.
  
  
  METHODS
  In the light of that the ligand of Con A is mannoses and that the function of alpha-mannosidase is trimming of mannoses in the oligosaccharide chains of glycoproteins, COS-7 cells and CNE-2L2 cells, a cloned cell line of human nasopharyngeal carcinoma, were transfected with pRc/CMV-sense 6A8 cDNA or pRc/CMV-antisense 6A8 cDNA and the Con A binding to the cells was examined upon a lazer scan confocal microscope.
  
  
  RESULTS
  The data demonstrated that with transfection of pRc/CMV-sense 6A8 cDNA the binding of Con A to the cells was decreased, but the staining of the cells with mAb 6A8 was enhanced, and vise versa for the cells with transfection of pRc/CMV-antisense 6A8 cDNA.
  
  
  CONCLUSIONS
  The protein encoded by 6A8 cDNA might be a type of alpha-mannosidase recognized by mAb 6A8.
authors.affiliation: Department of Immunology, Insitutute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
authors.forename: G
authors.initials: G
authors.lastname: Shi
authors.forename: F
authors.initials: F
authors.lastname: Ma
authors.forename: L
authors.initials: L
authors.lastname: Zhu
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: F
authors.initials: F
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D019556:COS Cells; D016373:DNA, Antisense / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics*; D006801:Humans; D019169:Jurkat Cells; D008253:Macaca mulatta; D008361:Mannosidases / Q000096:biosynthesis / Q000235:genetics*; D018613:Microscopy, Confocal; D009303:Nasopharyngeal Neoplasms / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured; D043323:alpha-Mannosidase
chemical_list: D000911:Antibodies, Monoclonal; D016373:DNA, Antisense; D018076:DNA, Complementary; D008361:Mannosidases; D043323:alpha-Mannosidase