title: The microflora recovered from the outer-surfaces of the Frialit-2 implanto-prosthetic connector.
abstract: The aim of the present investigation was to examine the outer-surface microbiota of the prosthetic connector of Frialit-2 implants, and to compare the microbial findings with the peri-implant parameters 2 years after functional loading. In 16 implant-treated patients (55.8 +/- 9.5 years) the outer-surface micro-organisms of the prosthetic connectors were determined in 32 Frialit-2 implants. The functional loading time of the prosthetic suprastructures was 24.1 +/- 13.8 months on average. After removal of the implant-supported restoration, microbial samples were obtained from the outer-surfaces of the Frialit-2 prosthetic connector. The microbial plaque samples were specified on CDC-blood agar as percentages of the total cultivable flora. Actinobacillus actinomycetemcomitans was semiquantitively determined on TSBV-agar in CFU/ml. The microbial plaque samples were dominated by Actinomyces israelii (68.8%), Eubacterium lentum (56.3%) and Veillonella parvula (43.8%) with proportions ranking between 3.9% (V. parvula) and 11.1% (A. israelii). The most frequently detected gram-negative microorganisms were Fusobacterium nucleatum (87.5%), Porphyromonas gingivalis (81.3%), and Peptostreptococcus micros (68.8%) with enhanced proportions for P. gingivalis (11.4%) and P. micros (11.4%). No statistical significant correlation could be established between the microbiota present on the outer-surfaces of the F2-connector and the peri-implant parameters examined. The outer-surface microflora recovered from the implanto-prosthetic-connector of Frialit-2 implants reveals a colonization with gram-positive bacteria and potentially harmful gram-negative micro-organisms that were frequently detected, but present at low levels. After 2 years of restorative loading, the outer-surface microbial colonization is compatible with peri-implant soft tissue health.
authors.affiliation: Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, USA. rainer-buchmann@yahoo.de
authors.forename: Rainer
authors.initials: R
authors.lastname: Buchmann
authors.forename: Fouad
authors.initials: F
authors.lastname: Khoury
authors.forename: Dieter
authors.initials: D
authors.lastname: Pingel
authors.forename: Dieter E
authors.initials: DE
authors.lastname: Lange
mesh_terms: D000190:Actinomyces / Q000145:classification; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification; D015169:Colony Count, Microbial; D015921:Dental Implants / Q000382:microbiology*; D003773:Dental Plaque / Q000382:microbiology; D017267:Dental Prosthesis Design; D019094:Dental Prosthesis, Implant-Supported / Q000382:microbiology*; D005051:Eubacterium / Q000145:classification; D005260:Female; D005500:Follow-Up Studies; D016967:Fusobacterium nucleatum / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D010462:Peptostreptococcus / Q000145:classification; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D018709:Statistics, Nonparametric; D013499:Surface Properties; D014678:Veillonella / Q000145:classification; D016474:Weight-Bearing
chemical_list: D015921:Dental Implants