[Effects of nao-yi-an granule on the intercellular expression of IL-6 in the experimental intracerebral hemorrhagic brain of rats].
OBJECTIVE
To define the cellular localization of IL-6 and the effects of the traditional Chinese medicine complex nao-yi-an granule (NYA) on the intercellular expression of IL-6 in the experimental intracerebral hemorrhagic (ICH) brain of rats, so as to investigate the efficacy mechanisms of NYA on ICH.


METHODS
ICH rat models were established by stereotaxically injecting 0.4 U type VII collagenase into the right globus pallidus (GP). The distribution and content of IL-6 protein expression together with the reaction of glial cells were investigated with the immunohistochemistry method.


RESULTS
Microglial activation was detected from 6 h to 4 d after ICH in the hematoma region, and reactive astrocytes were found from 6 h to 7 d after ICH around the hematoma region; there was an up-regulation of IL-6 in the hematoma at 6 h after ICH; it reached its peak at 12 h, and disappeared on 7 d.


CONCLUSIONS
The major sources of IL-6-positive protein were neurons and the activated microglia in the brain of ICH rats. Stabilizing the increasing expression of IL-6 may be one of the neuroprotective mechanisms of NYA.
Xiao
Lan
L
Institute of Combined Traditional Chinese Medicine and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.
Li
Xing-qun
XQ
Zhang
Hua-xian
HX
D000818:Animals; D001923:Brain Chemistry; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D020300:Intracranial Hemorrhages / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D012333:RNA, Messenger