2-aminopurine allows interspecies recombination by a reversible inactivation of the Escherichia coli mismatch repair system.
2-Aminopurine treatment of Escherichia coli induces a reversible phenotype of DNA mismatch repair deficiency. This transient phenotype results in a 300-fold increase in the frequency of interspecies conjugational recombination with a Salmonella enterica serovar Typhimurium Hfr donor. This method can be used for the generation of biodiversity by allowing recombination between diverged genes and genomes.
Matic
Ivan
I
INSERM U571, Faculté de Médecine, Necker-Enfants Malades, Université René Descartes, 75730 Paris Cedex 15, France. matic@necker.fr
Babic
Ana
A
Radman
Miroslav
M
D015075:2-Aminopurine / Q000494:pharmacology*; D000251:Adenosine Triphosphatases / Q000235:genetics; D001426:Bacterial Proteins; D020137:Base Pair Mismatch; D003227:Conjugation, Genetic / Q000187:drug effects*; D004260:DNA Repair; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics; D000070956:MutL Proteins; D051719:MutS DNA Mismatch-Binding Protein; D011995:Recombination, Genetic / Q000187:drug effects*; D012486:Salmonella typhimurium / Q000235:genetics*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C074770:MutL protein, E coli; D015075:2-Aminopurine; D000251:Adenosine Triphosphatases; D000070956:MutL Proteins; D051719:MutS DNA Mismatch-Binding Protein; C497175:MutS protein, E coli