[Comparative study on regulation patterns of compound prescriptions for kidney tonifying and for blood circulation activating on T-cell apoptosis related gene expression in aged rats].
OBJECTIVE
To investigate the regulation pattern of the two compound prescriptions for Kidney tonifying, Yougui Yin and Bushen Yishou capsule, in down-regulating T-cell apoptosis gene expression in aged rats.


METHODS
Expressions of T-cell apoptosis promoting and inhibiting genes, including Fas, FasL, Bcl-2, Bax, TNFR1 and TNFR2, as well as activity of cysteine proteinase in cascade connection, such as Caspase 8 and Caspase 3 were determined by TUNEL labeled flow cytometry and fluorescence real-time quantitative RT-PCR technique. The difference between old and young rats was compared, and the different regulation patterns of the two compound prescriptions for Kidney tonifying and their effects on Caspase activity were compared with those of compound prescription for blood circulation activating.


RESULTS
The two compound prescriptions for Kidney tonifying could effectively lower T-cell over-apoptosis in old rats, down-regulate FasL and TNFR1 gene transcription, and decrease the activity of Caspase 8 and Caspase 3, while the compound prescription for blood circulation activating showed insignificant effect on T-cell over-apoptosis.


CONCLUSIONS
Kidney-deficiency is closely related to the T-cell over-apoptosis. The T-cell over-apoptosis in old rats could be effectively improved by the two compound prescription for Kidney tonifying through down-regulating the apoptosis promoting genes FasL and TN-FR1 transcription. That is the unique regulation pattern of Kidney tonifying principle to T-cell apoptosis related gene in old rats.
Guo
Wei-min
WM
Institute of Integrated Traditional Chinese and Western Medicine, Medical Center of Fudan University, Shanghai 200040.
Shen
Zi-yin
ZY
Chen
Yu
Y
D000375:Aging / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001775:Blood Circulation / Q000502:physiology; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D053222:Fas Ligand Protein; D005260:Female; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis / Q000235:genetics; D013601:T-Lymphocytes / Q000166:cytology*; D016711:Yang Deficiency / Q000276:immunology; D019014:fas Receptor / Q000096:biosynthesis / Q000235:genetics
D004365:Drugs, Chinese Herbal; D053222:Fas Ligand Protein; C505662:Faslg protein, rat; D008562:Membrane Glycoproteins; D018124:Receptors, Tumor Necrosis Factor; D019014:fas Receptor; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases