[Nucleotide sequence at position -155 to +25 of the 5' flanking region of the angiotensinogen gene of Han people].
OBJECTIVE
To study the polymorphism of 5'-flanking region of angiotensinogen gene and relations to essential hyperfension.


METHODS
The nucleotide sequence at position -155 to +25 of the 5'-flanking region in angiotensinogen gene of Han people in Chinese population was identified by applying PCR-single stranded conformational polymorphism(SSCP) and PCR-directed sequencing.


RESULTS
(1) The Han people carry an adenylate(A), instead of a cytidylate(C) at position -20 of the 5'-flanking region of AGT gene; (2) A new mutation T-->A at position -46 was detected and A-allele frequencies was similar in both hypertensives and normotensive controls.


CONCLUSIONS
The variant T-->A at position -46 of AGT gene was not associated with hypertension, but an adenylate (A) at position -20 of the 5'-flanking region of AGT gene. might be an genetic marker for Hans people.
Cheng
X
X
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Qiu
C
C
Zhou
W
W
Zhu
X
X
Liu
Y
Y
D024506:5' Flanking Region / Q000235:genetics*; D000328:Adult; D000368:Aged; D000808:Angiotensinogen / Q000235:genetics*; D044466:Asian People; D002681:China; D005260:Female; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010375:Pedigree; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational
D000808:Angiotensinogen