[Determination of glycyrrihizin in rats plasma and pharmacokinetics after oral administration of si-jun-zi-tang].
OBJECTIVE
To study the pharmacokinetics of glycyrrhizin after oral administration of Si-jun-zi-tang in rats and provide the experimental evidences for the prescription pharmacokientics of traditional Chinese medicine.


METHODS
A RP-HPLC method was established for rapid determination of glycyrrhizin in rats plasma. After obtaining the blood sample at different time, the plasma concentration of GL were detected respectively. The data were processed by the software named 3P87 to find the pharmacokinetic parameters.


RESULTS
A good linear relationship existed in the range of GL from 1.224 micrograms/ml to 38.656 micrograms/ml. Whole blood concentration-time course of GL after oral administration Si-jun-zi-tang was fitted to be a two-compartment model. GL in rats plasma which came from Si-jun-zi-tang was absorbed and distributed rapidly, but excreted slowly.


CONCLUSIONS
This RP-HPLC method was sensitive, raliable and rapid, it can be used in absorption and metabolism of GL, also in detection of GL preparation. This experiment is prepared for further study of the tradtional Chinese medicine prescription pharmacokinetics.
Zhang
L
L
Xijing Hospital, Fourth Military Medical University, Xi'an 710032.
Huang
X
X
Wang
L
L
Reng
P
P
Feng
J
J
Chen
J
J
D000284:Administration, Oral; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D019695:Glycyrrhizic Acid / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D000893:Anti-Inflammatory Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D019695:Glycyrrhizic Acid