[Experimental studies on the anti-thrombosis effect of 3,4-oxo-isopropylidene-shikimic acid].
OBJECTIVE
To study the effect of 3,4-oxo-isopropylidene-shikimic acid (ISA) against arteriovenous shunt and middle cerebral artery thrombosis (MCAT) in rats.


METHODS
Arteriovenous shunt model was adopted to measure thrombus weight; The neurologic deficit (ND) and the infarct size (IS) of the middle cerebral thrombosis (MCAT) model induced by FeCl3 were observed; The effect of ISA on platelet aggregation rate of rat and rabbit by giving ISA in vivo and in vitro was studied.


RESULTS
ISA 25, 50, 100 and 200 mg.kg-1 ig was shown to reduced the weight of thrombus in arteriovenous shunt model; ISA 50, 100 and 200 mg.kg-1 ig for 2 times in 24 hours, attenuated the ND of rats subjected to MCAT; ISA 100 and 200 mg.kg-1 reduced IS of rats after MCAT by 27.8% and 31.6%, respectively; ISA 50, 100 and 200 mg.kg-1 ig inhibited platelet aggregation of normal rats; ISA 10(-3)-10(-5) mol.L-1, inhibited rabbit platelet aggregation in vitro.


CONCLUSIONS
ISA inhibited thrombosis by anti-platelet-aggregation.
Wang
Hong-tao
HT
Department of Pharmacology, College of Chinese Medicine, Beijing University of Traditional Chinese Medicine, Beijing 100102, China. wht72@263.net
Jin
Hong-tao
HT
Sun
Jian-ning
JN
Xu
Qiu-ping
QP
Guo
Ya-jian
YJ
D000818:Animals; D001921:Brain / Q000473:pathology; D004195:Disease Models, Animal; D005343:Fibrinolytic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D020244:Infarction, Middle Cerebral Artery / Q000097:blood* / Q000473:pathology / Q000517:prevention & control; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012765:Shikimic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification*
C479295:3,4-oxoisopropylideneshikimic acid; D005343:Fibrinolytic Agents; D012765:Shikimic Acid