[Determination of ampelopsin in the different parts of Ampelopsis grossedentata in different seasons by RP-HPLC].
OBJECTIVE
To determine ampelopsin in the root, stem and leaf of Ampelopsis grossedentata in different seasons by RP-HPLC.


METHODS
The determination was carried out with ODS column (5 microns, 250 mm x 46 mm), using methanol-water-phosphoric acid(25:75:0.1) as the mobile phase at a flow rate of 1.0 ml/min and detected at the wavelength 289 nm.


RESULTS
Ampelopsin content was in the order of leaf > stem > root. Ampelopsin content in Ampelopsis grossedentata collected in April and May was the highest.
Wang
Yan
Y
Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405.
Zhou
Liling
L
Li
Rui
R
Wang
Ying
Y
D032421:Ampelopsis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012621:Seasons
D005419:Flavonoids; C106407:ampelopsin