Variations in flow, duration, and concentration do not change the final lung concentration of melphalan after isolated lung perfusion in rats.
OBJECTIVE
The influences of flow, perfusion time, and concentration on melphalan (MN) lung levels in a rat model of isolated lung perfusion (ILuP) were studied.


METHODS
ILuP was performed in WAG/Rij rats by using a single-pass system with 0.5, 0.05, 0.01, and 0.005 mg of MN. Subsequently ILuP with 0.05 mg MN was performed during 30 min with a flow rate of 0.5 ml/min, during 60 min with a flow rate of 0.5 ml/min, and during 30 min with a flow rate of 1.0 ml/min.


RESULTS
The lung MN levels of 0.05 mg were significantly lower as compared with 0.5 mg (p = 0.02). In the second experiment, no significant differences were seen in lung levels of MN between the different groups.


CONCLUSIONS
The final lung MN levels were only determined by the absolute amount administered.
Romijn
S
S
Department of Thoracic and Vascular Surgery, University of Antwerp, Belgium.
Hendriks
J M H
JM
Van Putte
B P
BP
Guetens
G
G
De Bruijn
E A
EA
Van Schil
P E Y
PE
D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008168:Lung / Q000378:metabolism*; D008297:Male; D008558:Melphalan / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D009994:Osmolar Concentration; D010477:Perfusion; D011652:Pulmonary Circulation; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D018906:Antineoplastic Agents, Alkylating; D008558:Melphalan