[Construction and characterization of the cDNA library from Schistosoma japonicum cercariae].
OBJECTIVE
To construct a cDNA library from Schistosoma japonicum cercariae.


METHODS
Total RNA was extracted from 500,000 S. japonicum cercariae and used to synthesize the double-stranded cDNA and cloned into the TriplEx2 vector using "SMART cDNA Construction Kit".


RESULTS
The primary titer of the constructed cDNA library is 1.8 x 10(7) pfu/ml and the titer of amplified library is 2.5 x 10(10) pfu/ml. The average size of inserts is 1.075 kb. The recombinant efficiency is 94.4%. The full length cDNA of S. japonicum TPI and JF-2 genes was successfully amplified from the library.


CONCLUSIONS
A cDNA library of S. japonicum cercariae has been constructed successfully.
Chen
Xiao-guang
XG
Department of Parasitology, Institute of Tropical Medicine, First Military Medical University, Guangzhou 510515.
Li
Hua
H
Peng
Hong-juan
HJ
Shen
Shu-man
SM
Fung
Ming-chiu
MC
D000818:Animals; D015723:Gene Library; D012549:Schistosoma japonicum / Q000235:genetics*