title: Mechanism of cis-trans isomerization of unsaturated fatty acids in Pseudomonas putida.
abstract: We studied the pattern of the cis-trans isomerization of unsaturated fatty acids in cells of Pseudomonas putida S12 grown in a medium supplemented with oleic acid which was deuterated at both of the C atoms of its double bond. Direct evidence that isomerization does not include a transient saturation of the double bond was obtained. In addition, analysis of the amino acid sequences of the seven known Cti proteins identified them as heme-containing proteins of the cytochrome c type.
authors.affiliation: Institut für Mikrobiologie, Westfälische Wilhelms-Universität Münster, 48149 Münster, Germany.
authors.forename: Angelika
authors.initials: A
authors.lastname: von Wallbrunn
authors.forename: Hans Hermann
authors.initials: HH
authors.lastname: Richnow
authors.forename: Grit
authors.initials: G
authors.lastname: Neumann
authors.forename: Friedhelm
authors.initials: F
authors.lastname: Meinhardt
authors.forename: Hermann J
authors.initials: HJ
authors.lastname: Heipieper
mesh_terms: D001426:Bacterial Proteins; D002462:Cell Membrane / Q000378:metabolism; D003903:Deuterium; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000378:metabolism*; D007536:Isomerism; D019301:Oleic Acid / Q000737:chemistry / Q000378:metabolism; D010802:Phylogeny; D016958:Pseudomonas putida / Q000378:metabolism*; D016415:Sequence Alignment; D019745:cis-trans-Isomerases / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D005231:Fatty Acids, Unsaturated; D019301:Oleic Acid; D003903:Deuterium; D019745:cis-trans-Isomerases; C121604:cti protein, Pseudomonas putida