title: Topical application of benzalkonium chloride to the stomach serosa increases gastric emptying time, acid secretion, serum gastrin and size of the mucosa.
abstract: OBJECTIVE
  In the present study we evaluated the effects of gastric myenteric denervation using benzalkonium chloride (BAC) on the time for gastric emptying, as well as gastric secretion, and mucosal epithelial cell size and population in rats.
  
  
  RESULTS
  Wistar rats were treated with topical serosal application of BAC to the stomach. Control animals received saline. Ninety days after surgery, gastric emptying time, gastric acid secretion and serum gastrin levels were studied. Next, the animals were sacrificed and the stomachs were removed, fixed in formalin and histologically processed for histomorphometry of the height, area and volume of the glandular portion, and volume and population of mucous, chief, parietal, G- and labelled cells. BAC animals showed a significant delay in gastric emptying and an increase in gastric acid secretion and serum gastrin levels. These animals also presented a significant reduction of myenteric neuron number, hypertrophy of parietal and chief cells, hyperplasia of G cells and an increase in the gastric mucosa area.
  
  
  CONCLUSIONS
  The absence of the myenteric plexus seems to protect the stomach from the hyperplastic effects of hypergastrinemia. Gastric food stasis may act as a factor triggering morphological and functional alterations of the gastric epithelium. Although gastric food stasis is a common finding in medical practice, its physiopathological consequences are poorly understood and have not been frequently discussed in the literature.
authors.affiliation: Department of Pathology, Faculty of Medicine of Ribeirão Preto,University of São Paulo, Brazil. szucolot@fmrp.usp.br
authors.forename: S
authors.initials: S
authors.lastname: Zucoloto
authors.forename: L M F
authors.initials: LM
authors.lastname: Romanello
authors.forename: S B
authors.initials: SB
authors.lastname: Garcia
authors.forename: L F R
authors.initials: LF
authors.lastname: Sobreira
authors.forename: A J A
authors.initials: AJ
authors.lastname: Barbosa
authors.forename: L E A
authors.initials: LE
authors.lastname: Troncon
mesh_terms: D000818:Animals; D001548:Benzalkonium Compounds; D019872:Chief Cells, Gastric / Q000473:pathology; D003902:Detergents; D005744:Gastric Acid / Q000378:metabolism; D005745:Gastric Acidity Determination; D005746:Gastric Emptying; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D019863:Gastrin-Secreting Cells / Q000473:pathology; D005755:Gastrins / Q000097:blood*; D006965:Hyperplasia; D009121:Muscle Denervation / Q000379:methods; D009130:Muscle, Smooth / Q000294:innervation / Q000502:physiology; D009197:Myenteric Plexus / Q000187:drug effects; D009929:Organ Size; D010295:Parietal Cells, Gastric / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000294:innervation / Q000502:physiology*
chemical_list: D001548:Benzalkonium Compounds; D003902:Detergents; D005755:Gastrins