Effects of Tryptergium Wilfordii Polyglyco-sidium on the tumour necrosis factor levels in aqueous humor after intraocular lens implantation.
OBJECTIVE
Effects of Tryptergium Wilfordii Polyglyco-sidium (TWP) on the Tumour Necrosis Factor (TNF) levels in aqueous humor after intraocular lens (IOL) implantation were studied in rabbits.


METHODS
Twenty-seven pigmented rabbits were divided into three groups. In the first group, the IOL were placed in the capsular bag after lens extraction. In the second group, rabbits received TWP therapy after IOL implantation. And in the third group, rabbit received prednisone therapy after IOL implantation. Aqueous humor samples were aspirated on the 1st, 3rd, 7th, and 14th postoperative day. A modified double antibodies indirect sandwich ELISA was used to detect for the presence of TNF. The data were closely studied by means of analysis of variance with SAS software.


RESULTS
It was found that TNF level in aqueous humor reached its maximum on the 7th postoperative day in the group with IOL implantation. It was also found that the TNF levels in aqueous humor on the 7th and 14th postoperative day were significantly lower (P < 0.05) in the TWP-treated group with IOL implantation than those in the non-treatment group and the steroid-treated group.


CONCLUSIONS
The current study offers evidence that TWP is more effective than the steroid in reducing the TNF level in aqueous humor after IOL implantation.
Zhou
Z
Z
Department of Ophthalmology, No. 304 Hospital, Beijing 100037, China.
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D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D019654:Lens Implantation, Intraocular; D011108:Polymers / Q000494:pharmacology; D011184:Postoperative Period; D011817:Rabbits; D029757:Tripterygium / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D011108:Polymers; D014409:Tumor Necrosis Factor-alpha; C046409:polyglyconate