title: [Fractionation and identification of Schistosoma japonicum adult worm 67kD protein].
abstract: OBJECTIVE
  To isolate the specific protein bands which react to sera from rabbits infected with Schistosoma japonicum (Sj) and those immunized with Sj adult worm antigens (AWA) for immunodiagnosis of schistosomiasis.
  
  
  METHODS
  AWA was analysed using SDS-PAGE and immunoblotting, and 67 kD protein was fractionated by electrophoretic chromatography.
  
  
  RESULTS
  67 kD protein which reacted to rabbit sera infected with Sj and immunized with AWA was obtained after the fractionation of AWA through electrophoretic chromatography.
  
  
  CONCLUSIONS
  Electrophoretic chromatography is a useful tool for the fractionation of schistosome antigens.
authors.affiliation: Laboratory of Schistosomiasis, Xiangya School of Medicine, Central South University, Changsha, 410078, China.
authors.forename: Shi-shan
authors.initials: SS
authors.lastname: Yuan
authors.forename: Xian-fang
authors.initials: XF
authors.lastname: Zeng
authors.forename: Yue-long
authors.initials: YL
authors.lastname: Huang
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis / Q000302:isolation & purification*; D004586:Electrophoresis; D015801:Helminth Proteins / Q000032:analysis / Q000302:isolation & purification*; D011817:Rabbits; D012549:Schistosoma japonicum / Q000737:chemistry / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000469:parasitology
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins