[The effect of erycibele alkaloid on intracellular Ca2+ mobilation of cultured human ciliary muscle cells].
OBJECTIVE
To study the effect of erycibele alkaloid on cultured human ciliary muscle intracellular Ca2+ movement.


METHODS
Intracellular Ca2+ was studied by Fura-2 probe and muscarinic antangonist was applied.


RESULTS
Erycibele alkaloid had no any possible disturbulance on the examination system. The average resting[Ca2+]i was 79.3 +/- 28.7 nmol/Lol/L (n = 24). Erycibele alkaloid caused biphase [Ca2+]i elevation. Non-selective muscarinic antagonist Atropine (1 nmol/L) could completely block the 1 mumol/L erycibele alkaloid induced [Ca2+]i elevation; M1 antagonist Pirenzipine could not block the 1 mumol/L erycibele alkaloid induced [Ca2+]i elevation in low concentration (0.03 nmol/L) but in higher concentration (1 nmol/L). M3 antagonist 4-diphenyl acetoxy N-methy piperidine methiodide could completely block the 1 mumol/L erycibele alkaloid induced [Ca2+]i elevation in low and high concentration.


CONCLUSIONS
The effect of erycibele alkaloid on increasing cultured human ciliary muscle cell intracellular Ca2+ is mediated by M3 receptor subtype.
Huang
W
W
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Peng
D
D
Zeng
S
S
Li
S
S
D001693:Biological Transport, Active; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D002924:Ciliary Body / Q000166:cytology* / Q000378:metabolism; D016257:Fura-2; D006801:Humans; D018721:Muscarinic Agonists / Q000494:pharmacology*; D011976:Receptors, Muscarinic / Q000187:drug effects; D014326:Tropanes / Q000494:pharmacology*
D019086:Bridged Bicyclo Compounds, Heterocyclic; D018721:Muscarinic Agonists; D011976:Receptors, Muscarinic; D014326:Tropanes; C027863:bao gong teng A; D002118:Calcium; D016257:Fura-2