[The difference in proliferation and differentiation ability of cultured marrow stromal cells between 3-month-old and 12-month-old rats in vitro].
OBJECTIVE
To compare the difference in proliferation and differentiation (to osteoblast or adipocyte) ability of cultured rat marrow stromal cells (rMSCs) between 3-month-old and 12-month-old rats in vitro.


METHODS
The rMSCs of 3-month-old and 12-month-old were cultured in vitro. The growth curves were depicted to compare their proliferation ability. Both 3-month-old and 12-month-old rMSCs were induced to osteoblasts or adipocytes by osteogenic inducer or adipogenic inducer. At different times, these cells were observed by histochemistry staining.


RESULTS
The growing ability of both passage 4 (P4) and passage 9 (P9) of 12-month-old rMSCs decreased in comparison with that of 3-month-old rMSCs. The growing ability of P9 was less than that of P4 of 12-month-old rMSCs. The expression of alkaline phosphatase (ALP) of the control group and induced group of 12-month-old rMSCs was less than that of 3-month-old rMSCs after 1-week osteogenic induction. 12 days later, calcification was observed in 3-months-old group. Lipid droplets occurred in the cells of 12-month-old group after 2-day adipogenic induction, whereas the droplets occurred after 3-day or 4-day induction in the 3-month-old group.


CONCLUSIONS
The ability of proliferation and osteogenesis of 12-month-old group is weaker than that of 3-month-old group, but the ability of adipogenesis of 12-month-old group is stronger.
Yao
Rong
R
Institute of Biomedical Engineering, West China Center of Medical Sciences, Sichuan University, Chengdu 610041, China.
Chen
Huaiqing
H
Deng
Li
L
Li
Liang
L
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000201:enzymology; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D005260:Female; D010012:Osteogenesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000166:cytology* / Q000201:enzymology
D000469:Alkaline Phosphatase