[Studies on the determination of serum thymic factor by capillary zone electrophoresis].
The determination of serum thymic factor (FTS) by capillary zone electrophoresis was described. The optimum ionic concentration and the pH value of buffer system was examined through orthogonal analysis. The optimum conditions found to be 0.05 mol/L sodium tetraborate, 0.01 mol/L phosphate buffer at pH 8.70, working voltage at 12 kV and wavelength at 200 nm. The recoveries for FTS standards added to the bovine serum albumin and serum samples were measured. The values of the relative standard deviation(RSD), recovery and detection limit were 7.62%, 80.28% and 40 ng/ml respectively. The development of this method provide another way for studying the relations of zinc and thymic factor and investigating the effect of zinc on immune function.
Qu
N
N
Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China.
Chen
J
J
Xia
Y
Y
D019075:Electrophoresis, Capillary; D006801:Humans; D013935:Thymic Factor, Circulating / Q000032:analysis*
D013935:Thymic Factor, Circulating