title: Dose-dependent and preterm- accentuated diaplacental transport of nutritive allergens in vitro.
abstract: OBJECTIVE
  The mechanisms by which nutritive allergens are transported from mother to fetus and the ensuing immunological response are incompletely understood. We investigated the role of different allergen concentrations in influencing the diaplacental allergen transport in preterm and term placentas.
  
  
  METHODS
  Twenty-seven human term placentas and 12 preterm placentas were dually perfused in vitro for up to 4 h by adding alternately two different nutritive allergens, beta-lactoglobulin (BLG) or ovalbumin (OVA), at four different allergen concentrations (0.02, 0.2, 2 and 20 mg/ml) to the maternal perfusate medium. Allergen concentrations in fetal venous outflow samples collected during perfusion were measured by using specific ELISAs.
  
  
  RESULTS
  Perfusion of increasing allergen concentrations via the maternal circulation resulted in a concentration-dependent increase of fetal allergen uptake in all term and preterm placentas. A mean maternal-to-fetal ratio of 20,000/1 and 3,000/1 for BLG, and 40,000/1 and 5,000/1 for OVA was found in term and preterm placentas, respectively. Preterm placentas (27-36 weeks of gestation) were found to favor the diaplacental passage of nutritive allergens compared with placentas at term (>36 weeks of gestation).
  
  
  CONCLUSIONS
  Maternal-to-fetal allergen transport occurs in a dose-dependent and molecular weight-dependent manner with clear accentuation in preterm placentas.
authors.affiliation: Department of Pediatrics, University of Vienna, Austria.
authors.forename: Monika
authors.initials: M
authors.lastname: Edelbauer
authors.forename: Christine
authors.initials: C
authors.lastname: Loibichler
authors.forename: Armin
authors.initials: A
authors.lastname: Witt
authors.forename: Marianne
authors.initials: M
authors.lastname: Gerstmayr
authors.forename: Bettina
authors.initials: B
authors.lastname: Putschögl
authors.forename: Radvan
authors.initials: R
authors.lastname: Urbanek
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Szépfalusi
mesh_terms: D000485:Allergens / Q000378:metabolism*; D001692:Biological Transport; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005865:Gestational Age; D006801:Humans; D008431:Maternal-Fetal Exchange; D008970:Molecular Weight; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011312:Pressure
chemical_list: D000485:Allergens