[Association of polymorphisms of vitamin D receptor gene start codon and 3'-end region with bone mineral density in postmenopausal women].
OBJECTIVE
To determine whether vitamin D receptor(VDR) gene start codon polymorphisms and 3'-end region polymorphisms exerted a combined influence on bone mineral density(BMD) in Han postmenopausal women in Beijing area.


METHODS
The VDR Fok I and 3'-end region genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism in 110 unrelated postmenopausal women. BMD was measured at the lumbar spine (L(2-4)), femoral neck(Neck), Ward's triangle(Ward's) and trochanter (Troch) using duel-energy X-ray absorptiometry.


RESULTS
The frequencies distribution of Fok I, Apa I, Bsm I and Taq I alleles in this cohort all followed the Hardy-Weinberg equilibrium. No significant association of Fok I, Apa I or Taq I genotype with BMD in postmenopausal women was found when these polymorphisms were considered independently, except for Bsm I genotype. When a combined analysis of VDR gene Fok I and 3'-end region polymorphisms was carried out, cross-genotyping Fok I and Apa I polymorphisms was significantly associated with BMD at the L(2-4) (P<0.001), and cross-genotype of Fok I and Taq I was also significantly associated with BMD at the Neck and Troch sites (P<0.05). However, cross-genotyping Fok I and Bsm I polymorphisms was not significantly associated with BMD. Cross-genotyping Apa I and Bsm I or Taq I polymorphisms was not associated with BMD in postmenopausal women, either.


CONCLUSIONS
Although Fok I polymorphisms of VDR gene were not significantly associated with BMD in postmenopausal women, VDR gene Fok I and 3'-region polymorphisms (Apa I and Taq I) had a combined effect on the BMD in postmenopausal women.
Zhang
Zhen-lin
ZL
Department of Endocrinology, PUMC Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, PR China. zhenlinz66@yahoo.com.cn
Zhao
Jin-xiu
JX
Meng
Xun-wu
XW
Zhou
Xue-ying
XY
Xing
Xiao-ping
XP
Xia
Wei-bo
WB
D024509:3' Flanking Region / Q000235:genetics*; D000368:Aged; D000704:Analysis of Variance; D015519:Bone Density / Q000502:physiology*; D002681:China; D018387:Codon, Initiator / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017698:Postmenopause / Q000235:genetics* / Q000502:physiology; D018167:Receptors, Calcitriol / Q000235:genetics*
D018387:Codon, Initiator; D018167:Receptors, Calcitriol; D004247:DNA; D004262:DNA Restriction Enzymes