[Study on the scavenging action of polysaccharide of Usnea longissima to oxygen radical and its anti-lipi peroxidation effects].
OBJECTIVE
To study the scavenging action of polysaccharide of Usnea longissima Ach. (PUS) to oxygen free radical and its inhibiting action on lipidperoxidation.


METHODS
The scavenging action to superoxide anion free radical (O2-.) was tested by xanthine-xanthine oxidase system. The scavenging action on hydroxyl free racical (OH.) and anti-lipidperoxidation effects were tested by Fenton reaction.


RESULTS
The scaverging action was represented by the content scavenging 50% of all the free radical(IC50). The IC50 for O2-. and OH. were 0.45 mg/ml and 1.57 mg/ml respectively. The lipidperoxidation induced by OH. was weakly inhibited by PUS.


CONCLUSIONS
PUS can apparently scavenge oxygen free radical. But it can only weakly inhibit the lipid peroxidation of hepatocyte homogenate of mice which resulted form hydroxyl free radical.
Bian
Xiaoli
X
College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061.
Jin
Juqing
J
Ding
Dongning
D
Zhang
Hongmei
H
D000818:Animals; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism; D051379:Mice; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D020949:Usnea / Q000737:chemistry*
D016166:Free Radical Scavengers; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D017665:Hydroxyl Radical