title: Comparison of the 5.8S rRNA gene and internal transcribed spacer regions of trichomonadid protozoa recovered from the bovine preputial cavity.
abstract: Sequence analysis of the 5.8S rRNA gene and the internal transcribed spacer regions (ITSRs) was used to compare trichomonadid protozoa (n = 39) of varying morphologies isolated from the bovine preputial cavity. A multiple sequence alignment was performed with bovine isolate sequences and other trichomonadid protozoa sequences available in GenBank. As a group, Tritrichomonasfoetus isolates (n = 7) had nearly complete homology. A similarity matrix showed low homology between the T. foetus isolates and other trichomonads recovered from cattle (<70%). Two clusters of trichomonads other than T. foetus were identified. Eighteen isolates comprised 1 group. These isolates shared >99% homology among themselves and with Pentatrichomonas hominis. The other non-T. foetus cluster (n = 14) did not exhibit a high degree of homology (<87%) with other bovine isolates or any of the trichomonad sequences available in GenBank. The sequence homology among isolates in that cluster was >99%, except for 1 isolate that varied from the others in both ITSRs (approximately 2% dissimilarity). Sequence analysis of the 5.8S rRNA gene and ITSRs was useful for comparing trichomonadid protozoa isolated from the bovine preputial cavity and demonstrated that 2 distinct types of trichomonads constituted the non-T. foetus isolates recovered from the bovine preputial cavity.
authors.affiliation: California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Walker
authors.forename: D C
authors.initials: DC
authors.lastname: Hayes
authors.forename: S J
authors.initials: SJ
authors.lastname: Sawyer
authors.forename: R W
authors.initials: RW
authors.lastname: Nordhausen
authors.forename: K A
authors.initials: KA
authors.lastname: Van Hoosear
authors.forename: R H
authors.initials: RH
authors.lastname: BonDurant
mesh_terms: D000818:Animals; D002417:Cattle / Q000033:anatomy & histology* / Q000469:parasitology*; D002418:Cattle Diseases / Q000469:parasitology*; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D014644:Genetic Variation; D008297:Male; D008969:Molecular Sequence Data; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012749:Sexually Transmitted Diseases / Q000469:parasitology / Q000662:veterinary; D014244:Trichomonas / Q000235:genetics* / Q000302:isolation & purification* / Q000648:ultrastructure; D014245:Trichomonas Infections / Q000469:parasitology / Q000662:veterinary*
chemical_list: D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S