title: Genetic differences between two strains of Xylella fastidiosa revealed by suppression subtractive hybridization.
abstract: Suppression subtractive hybridization was used to rapidly identify 18 gene differences between a citrus variegated chlorosis (CVC) strain and a Pierce's disease of grape (PD) strain of Xylella fastidiosa. The results were validated as being highly representative of actual differences by comparison of the completely sequenced genome of a CVC strain with that of a PD strain.
authors.affiliation: Department of Plant Pathology, University of Florida, Gainesville, Florida 32611, USA.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Harakava
authors.forename: Dean W
authors.initials: DW
authors.lastname: Gabriel
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015139:Blotting, Southern; D002957:Citrus / Q000382:microbiology*; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D027843:Vitis / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins