title: Use of the confocal laser scanning microscope in studies on the developmental biology of marine crustaceans.
abstract: Confocal Laser Scanning Microscope techniques have been applied to study the developmental biology of marine copepods and decapod larvae. The lipophylic probes DiI and DiOC(6) were used to study both the external and internal morphology of these crustaceans, whereas the same DiOC(6) and the specific nuclear probe Hoechst 33342 were used to study embryonic development of copepods in vivo. To distinguish viable from non-viable copepod embryos, the vital dye dichlorodihydrofluorescein diacetate (H(2)DCFDA) was used. Major advantages and difficulties in the use of these non-invasive techniques in studies of the reproductive biology of marine crustaceans are discussed.
authors.affiliation: Stazione Zoologica "Anton Dohrn," 80122 Napoli, Italy. buttino@alpha.szn.it
authors.forename: Isabella
authors.initials: I
authors.lastname: Buttino
authors.forename: Adrianna
authors.initials: A
authors.lastname: Ianora
authors.forename: Ylenia
authors.initials: Y
authors.lastname: Carotenuto
authors.forename: Valerio
authors.initials: V
authors.lastname: Zupo
authors.forename: Antonio
authors.initials: A
authors.lastname: Miralto
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000378:metabolism; D002232:Carbocyanines / Q000378:metabolism; D033342:Copepoda / Q000196:embryology* / Q000648:ultrastructure; D033364:Decapoda / Q000196:embryology* / Q000648:ultrastructure; D004625:Embryo, Nonmammalian / Q000648:ultrastructure*; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism; D007814:Larva / Q000648:ultrastructure; D018613:Microscopy, Confocal / Q000379:methods; D012623:Seawater
chemical_list: C035005:3,3'-dihexadecylindocarbocyanine; D001562:Benzimidazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; C007392:3,3'-dihexyl-2,2'-oxacarbocyanine; C017807:bisbenzimide ethoxide trihydrochloride