[Effect of reduqing on HL-60 cells in secreting tumor necrosis factor alpha production and on TNF alpha converting enzyme mRNA expression].
OBJECTIVE
To explore the molecular mechanism of inhibiting effects of Reduqing on the release of tumor necrosis factor alpha (TNF alpha).


METHODS
Using cytobiologic and molecular biologic technique to observe the effects of Reduqing on HL-60 cells in producing inflammatory cytokine secreting TNF alpha (sTNF alpha) and on mRNA expression of TNF alpha converting enzyme (TACE).


RESULTS
(1) Reduqing, diluted in ratio 1:30, could effectively inhibit the increased HL-60 production of sTNF-alpha induced by lipopolysaccharides (LPS); (2) Although no obvious effect on TACE was shown when Reduqing was applied alone on HL-60 cells, there was evident inhibitory effect of Reduqing on TACE mRNA expression enhancement induced by LPS.


CONCLUSIONS
Reduqing could have the double inhibitory effects both on sTNF-alpha production and on the gene expression of its key enzyme, i.e. TACE stimulated by LPS, suggesting that it might be a hopeful and excellent natural TACE inhibitor.
Wang
K F
KF
Institute of Integrated Traditional and Western Medicine, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Sciences and Technology, Wuhan 430030.
Li
M Z
MZ
Yang
Y Z
YZ
D051722:ADAM Proteins; D000072198:ADAM17 Protein; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018922:HL-60 Cells / Q000378:metabolism; D006801:Humans; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D051722:ADAM Proteins; D008666:Metalloendopeptidases; D000072198:ADAM17 Protein; C000606462:ADAM17 protein, human; C000606463:Adam17 protein, mouse