Reconstitution of lethally irradiated mice by cells isolated from adipose tissue.
It is suggested that hematopoietic stem cells (HSC) could be found in several tissues of mesodermic origin. Among these, adipose tissue can expand throughout adult life and its expansion is not only due to mature adipocyte hypertrophy but also to the presence of precursor cells in stroma-vascular fraction (SVF). Here we report that transplantation of cells isolated from mice adipose tissue can efficiently rescue lethally irradiated mice and results in a reconstitution of major hematopoietic lineages. Donor cells can be detected in blood and in hematopoietic tissues of recipient mice. Adipose tissue contains a significant percentage of CD34, CD45 positive cells, and SVF cells were able to give rise to hematopoietic colonies in methylcellulose. We demonstrate the presence of hematopoietic progenitors in adipose tissue by phenotypic and functional characteristics. Thus adipose tissue could be considered as an important and convenient source of cells able to support hematopoiesis.
Cousin
Béatrice
B
UMR 5018-CNRS, CHU Rangueil, Bat L1, 1 Avenue J. Poulhès, 31403, Toulouse Cedex, France. cousinb@toulouse.inserm.fr
André
Mireille
M
Arnaud
Emmanuelle
E
Pénicaud
Luc
L
Casteilla
Louis
L
D000273:Adipose Tissue / Q000166:cytology* / Q000276:immunology; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D001772:Blood Cell Count; D002469:Cell Separation; D005260:Female; D005434:Flow Cytometry; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011828:Radiation Chimera / Q000097:blood; D013234:Stem Cells / Q000166:cytology / Q000276:immunology
D018952:Antigens, CD34; D017493:Leukocyte Common Antigens