title: Optimum detection times for bacteria and yeast species with the BACTEC 9120 aerobic blood culture system: evaluation for a 5-year period in a Turkish university hospital.
abstract: We tracked and documented the time of positivity of blood cultures by using the BACTEC 9120 (Becton Dickinson Diagnostic Instrument Systems) blood culture system over a 5-year study period. A 7-day protocol of the incubation period was selected, and a total of 11156 blood cultures were evaluated. The clinically significant microorganisms (32.95%) were isolated in 3676 specimens. Gram-positive and -negative bacterial isolation rates were found to be 41.07 and 44.88%, respectively. Yeasts were found in 14.03% of all pathogens. Both the false-positivity and -negativity rates were very low (0.1 and 0.3%, respectively). The mean detection times for all of the pathogens were determined to be 19.45 h. Yeasts, nonfermentative gram-negative bacteria, and Brucella melitensis strains were isolated within 5 days. By taking these data into account, we decided to establish a 5-day-incubation protocol in our laboratory instead of the 7 days that are commonly used.
authors.affiliation: Department of Clinical Microbiology, Medical Faculty Hospital of Osmangazi University, 26480 Eskisehir, Turkey. gdurmaz@ogu.edu.tr
authors.forename: Gül
authors.initials: G
authors.lastname: Durmaz
authors.forename: Tercan
authors.initials: T
authors.lastname: Us
authors.forename: Aydin
authors.initials: A
authors.lastname: Aydinli
authors.forename: Abdurrahman
authors.initials: A
authors.lastname: Kiremitci
authors.forename: Nuri
authors.initials: N
authors.lastname: Kiraz
authors.forename: Yurdanur
authors.initials: Y
authors.lastname: Akgün
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D006785:Hospitals, University; D006801:Humans; D011933:Reagent Kits, Diagnostic; D013997:Time Factors; D014421:Turkey; D015003:Yeasts / Q000302:isolation & purification*
chemical_list: D011933:Reagent Kits, Diagnostic