Liposomal delivery of nucleic acids in vivo.
Optimization of cationic liposomal complexes for in vivo applications and therapeutics is complex involving many distinct components. These components include nucleic acid purification, plasmid design, formulation of the delivery vehicle, administration route and schedule, dosing, detection of gene expression, and others. This review will focus on optimization of these components for use in a variety of in vivo applications. Use of improved liposome formulations for delivery in vivo is valuable for gene therapy and would avoid several problems associated with viral delivery. Delivery of nucleic acids using liposomes is promising as a safe and non-immunogenic approach to gene therapy. Furthermore, gene therapeutics composed of artificial reagents can be standardized and regulated as drugs rather than as biologics. Optimizing all components of the delivery system will allow broad use of liposomal complexes to treat or cure human diseases or disorders.
Smyth Templeton
Nancy
N
Center for Cell and Gene Therapy, Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA. NANCYT@bcm.tmc.edu
D000818:Animals; D016503:Drug Delivery Systems / Q000379:methods*; D018014:Gene Transfer Techniques; D006801:Humans; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D009696:Nucleic Acids / Q000008:administration & dosage*; D009928:Organ Specificity; D014162:Transfection
D008081:Liposomes; D009696:Nucleic Acids