title: Polymorphism at the ovine beta3-adrenergic receptor locus: associations with birth weight, growth rate, carcass composition and cold survival.
abstract: The beta3-adrenergic receptors (ADRB3s) are predominantly found on the surface of adipocytes and are the major mediators of the lipolytic and thermogenic effects of high catecholamine concentrations. Polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) analysis of part of the ovine beta3-adrenergic receptor gene (ADRB3) intron was used to screen 12 large Merino half-sib families for sequence variation. Six different alleles that segregated in a Mendelian fashion were observed. The genetic basis for the allelic differences were identified by sequencing the ADRB3 (coding and non-coding regions) from animals that were homozygous for each of the alleles. Five sire lines (two Merino x Merino, two Merino x Coopworth, one Dorset Down x Coopworth) provided phenotypic and genotypic data used to ascertain the effects of allelic variation at the ADRB3 locus on birth weight, weaning weight, growth rate (up until weaning), carcass composition at 63 days post-weaning and cold survival. Statistical analyses within each half-sib family showed that in some sire lines (S13, S15, and S17) the inheritance of a particular allele was associated with increased birth weights and/or increased growth rates up until weaning. The inheritance of a particular sire allele was associated with fatter carcasses in sire line S16. Chi-squared analysis revealed the association of the E allele with cold survival and the D allele with cold-related mortality in sire line S14. Such associations support the hypothesis that ADRB3s are involved in energy homeostasis. With more research, the variation detected at the ADRB3 locus may assist in the genetic selection for desirable animal production traits.
authors.affiliation: Animal and Food Sciences Division, Lincoln University 8140, Canterbury, New Zealand.
authors.forename: R H
authors.initials: RH
authors.lastname: Forrest
authors.forename: J G H
authors.initials: JG
authors.lastname: Hickford
authors.forename: A
authors.initials: A
authors.lastname: Hogan
authors.forename: C
authors.initials: C
authors.lastname: Frampton
mesh_terms: D000064:Acclimatization / Q000235:genetics; D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D001724:Birth Weight / Q000235:genetics*; D001823:Body Composition / Q000235:genetics; D003080:Cold Temperature; D017931:DNA Primers; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D022702:Receptors, Adrenergic, beta-3 / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000235:genetics* / Q000254:growth & development
chemical_list: D017931:DNA Primers; D022702:Receptors, Adrenergic, beta-3