RAPD markers linked to brown planthopper Nilaparvatha lugens resistance locus in rice.
Brown plant hopper, a major pest in rice causes "hopper burn" in the field. The resistance gene for brown planthopper was mapped by using 20 recombinant inbred lines (RIL's) derived from a cross between resistant line Oryza. officinalis derivative (IR 54742-2-21-12-17-6) and a susceptible rice cultivar ASD 16 using bulked segregant analysis. On an average of 4 loci were amplified and two RAPD primers amplified loci that co-segregated with resistance/susceptibility. The segregating RAPD loci were mapped using Mapmaker programme into 13 groups. The expected and the 95% confidence level were found to be 15.2 and 47.7 cM respectively, confirming the location of the brown planthopper resistant gene on the region of chromosome 4. These RAPD markers will accelerate breeding programme for brown planthopper resistance.
Amudha
J
J
Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore 641003, India.
SreeRangasamy
S R
SR
Jayapragasam
M
M
Balasubramani
G
G
D000818:Animals; D002874:Chromosome Mapping; D020090:Chromosome Segregation; D004351:Drug Resistance; D008040:Genetic Linkage; D005819:Genetic Markers; D006430:Hemiptera / Q000235:genetics*; D012275:Oryza / Q000469:parasitology; D010935:Plant Diseases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
D005819:Genetic Markers