Identification and characterization of KvgAS, a two-component system in Klebsiella pneumoniae CG43.
A two-component system encoding gene cluster kvgAS that is present only in virulent Klebsiella pneumoniae CG43 was isolated and its sequence determined. RT-PCR and Southern analysis demonstrated that kvgAS is organized as an operon. No apparent effect of a kvgS deletion on bacterial virulence was observed in a mouse peritonitis model. In the presence of paraquat or 2,2-dipyridyl, the activity of kvgAS promoter in the kvgS mutant was found to be reduced to half of the level in the wild-type strain. The data suggest that the KvgAS system is autoregulated and plays a role in countering free radical stresses and sensing iron-limiting conditions.
Lai
Yi-Chyi
YC
Department of Life Science and Institute of Molecular Medicine, National Tsing Hua University, Hsin Chu, Taiwan.
Lin
Ging-Ting
GT
Yang
Shu-Li
SL
Chang
Hwan-You
HY
Peng
Hwei-Ling
HL
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D000071677:Histidine Kinase; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000472:pathogenicity*; D051379:Mice; D005810:Multigene Family / Q000235:genetics; D016296:Mutagenesis; D010538:Peritonitis / Q000382:microbiology; D011494:Protein Kinases / Q000235:genetics; D017422:Sequence Analysis, DNA; D014774:Virulence
D001426:Bacterial Proteins; D011494:Protein Kinases; D000071677:Histidine Kinase