title: [Experimental studies on antioxidation of extracts from several plants used as both medicines and foods in vitro].
abstract: OBJECTIVE
  The scavenging effects of 8 extracts from several plants used as both medicines and foods on oxygen free radicals were studied in vitro.
  
  
  METHODS
  Chemiluminescence (CL) method and ultra-weak luminescence technique were used, meanwhile, macrophage respiratiory burst was also carried out to evaluate the antioxidation reaction.
  
  
  RESULTS
  The scavenging activity on superoxide anions (O2-.) differed in the following order: extract of clove (CE) > black tea (BTE) > green tea(GTE) > grape peel/seed (GE) > or = oologn tea (OTE) > > tumeric (TE). In Fenton reaction for hydroxyl radicals (.OH), the order as follows: BTE > CE > or = GTE > GE > OTE > > TE. Most extracts have stronger eliminating efficiency on .OH than on O2-. except CE, which may scavenge the O2-. formation more efficiently. After macrophages (5 x 10(6) cells/ml) were stimulated with phorbol myristate acetate, BTE showed stronger inhibiting action than CE on respiratory burst in macrophages. The results of ultra-weak luminescence measurement indicated that the extracts could delay and depress the DNA damage in CuSO4-Phen-Vc-H2O2-DNA chemiluminescence system.
  
  
  CONCLUSIONS
  It suggests that the tested antioxidants could decrase the formation of O2-. or .OH, some have chain-breaking function and preventative function during DNA damage.
authors.affiliation: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & School of Basic Medicine Peking Union Medical College, Beijing 100005.
authors.forename: R
authors.initials: R
authors.lastname: Feng
authors.forename: W
authors.initials: W
authors.lastname: He
authors.forename: O
authors.initials: O
authors.lastname: Hirotomo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016166:Free Radical Scavengers; D017665:Hydroxyl Radical / Q000378:metabolism; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008264:Macrophages / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D010944:Plants / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism; D013662:Tea
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D013662:Tea; D013481:Superoxides; D017665:Hydroxyl Radical; D010100:Oxygen