title: Characterization of (1)H-(1)H distances in a uniformly (2)H,(15)N-labeled SH3 domain by MAS solid-state NMR spectroscopy (section sign).
abstract: In this communication, we demonstrate the feasibility of obtaining long-range (1)H-(1)H distance information by MAS solid-state NMR for a microcrystalline, uniformly (2)H,(15)N-labeled sample of a SH3 domain of chicken alpha-spectrin. The experiments yield NOESY-type spectra and rely on the favorable dispersion of the (15)N chemical shifts of the protein backbone. Perdeuteration of nonexchangeable sites is employed to simplify proton spin systems and to obtain multiple structural information. Two mixing schemes, (1)H-(1)H double quantum filtered Post-C7 and (1)H spin diffusion, are implemented to obtain quantitative (1)H-(1)H distance information. Post-C7 and spin diffusion cross-peak buildup rates are discussed for initial-rate fitting and in the framework of n = 0 rotational resonance (rotor driven spin diffusion), respectively. Different deuteration schemes were tested to find conditions where short-range (1)H-(1)H interactions are truncated (e.g., between H(N) and H(alpha)), but long-range interactions are retained (e.g., between H(N) and H(N)).
authors.affiliation: Institut für Organische Chemie und Biochemie II, TU München, Lichtenbergstrasse 4, D-85747 Garching, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Reif
authors.forename: Barth J
authors.initials: BJ
authors.lastname: Van Rossum
authors.forename: Federica
authors.initials: F
authors.lastname: Castellani
authors.forename: Kristina
authors.initials: K
authors.lastname: Rehbein
authors.forename: Anne
authors.initials: A
authors.lastname: Diehl
authors.forename: Hartmut
authors.initials: H
authors.lastname: Oschkinat
mesh_terms: D000818:Animals; D002645:Chickens; D003903:Deuterium; D007553:Isotope Labeling / Q000379:methods; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D013049:Spectrin / Q000737:chemistry*; D018909:src Homology Domains
chemical_list: D009587:Nitrogen Isotopes; D013049:Spectrin; D003903:Deuterium