A human placental polydeoxyribonucleotide (PDRN) may promote the growth of human corneal fibroblasts and iris pigment epithelial cells in primary culture.
The optimal concentration of a human placental polydeoxyribonucleotide (PDRN) preparation (100 microg/ml) enhances the growth of human corneal fibroblasts in primary culture depending upon the donor age. In particular, this effect is very consistently reproducible with donors over 60 years of age (p = 0.0028), suggesting a selective benefit of PDRN in the tissue culture of senescent cells. Moreover, this drug may promote the development of human iris pigment epithelium (IPE) cells with much lower concentrations of fetal bovine serum than those suitable for the culture of IPE. Lastly, the use of a 'gauze disk' on the pieces of the corneal explants improves the efficiency of growth of the control fibroblast primary cultures.
Muratore
O
O
'DI.S.C.A.T., C.A. Romanzi Division of Microbiology, University of Genoa, Largo Rosanna Benzi 10, 1-16132 Genoa, Italy.
Cattarini
G
G
Gianoglio
S
S
Tonoli
E L
EL
Saccà
S C
SC
Ghiglione
D
D
Venzano
D
D
Ciurlo
C
C
Lantieri
P B
PB
Schito
G C
GC
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D002455:Cell Division / Q000187:drug effects; D003315:Cornea / Q000166:cytology / Q000187:drug effects*; D046508:Culture Techniques; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007498:Iris / Q000166:cytology / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000187:drug effects; D010920:Placenta / Q000737:chemistry*; D011089:Polydeoxyribonucleotides / Q000494:pharmacology*
D011089:Polydeoxyribonucleotides