title: [Experimental study on the apoptotic effect of arsenic trioxide on human osteosarcoma MG-63 cells].
abstract: OBJECTIVE
  To study the apoptotic effect of arsenic trioxide(As2 O3) on human ostrosarcoma MG-63 cells.
  
  
  METHODS
  The MTT reduction test, transmission electron microscopy and agarose gel electrophoresis were applied to observe the apoptotic changes of human osteosarcoma line MG-63 induced by As2 O3.
  
  
  RESULTS
  Typical apoptotic cellular changes were found after As2 O3 treatment. Transmission electron miroscopy showed that nucleolus disappeared and chromatin condensed under the nucleonic membrane. Agarose gel electrophorsis showed a "ladder" strand of DNA, a special phenomenon of cellular apoptosis. The above-mentioned changes were not found in the control group.
  
  
  CONCLUSIONS
  As2 O3 can induce apoptosis of human osteosarcoma MG-63 cells. Further studies are to be carried out on its mechanism.
authors.affiliation: Department of Othopaedics, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xiao
authors.forename: Kang-hua
authors.initials: KH
authors.lastname: Li
authors.forename: Jian-zhen
authors.initials: JZ
authors.lastname: Fang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D001859:Bone Neoplasms / Q000473:pathology; D006801:Humans; D012516:Osteosarcoma / Q000473:pathology*; D010087:Oxides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D001152:Arsenicals; D010087:Oxides; D000077237:Arsenic Trioxide