title: Changes in N-glycosylation of human stromal cells by telomerase expression.
abstract: It was established that remarkable changes in the N-glycosylation are induced in immortalized cancer cells. Whether changes were induced in human stromal cells immortalized by transfection with the human telomerase catalytic subunit (hTert) cDNA was examined by lectin blot analysis. Morphological appearance and growth rate of the gene-transfected stromal cells were not changed significantly. However, lectin blot analysis of membrane glycoprotein samples showed that bindings of Ricinus communis agglutinin-I (RCA-I) and of leuko-agglutinating phytohemagglutinin to glycoprotein bands increase significantly in the gene-transfected cells. No lectin binding was observed when blotted filters were treated with diplococcal beta-1,4-galactosidase or N-glycanase prior to incubation with RCA-I. In contrast, no changes in Coomassie brilliant blue-staining and in binding of concanavalin A were obtained between the primary and gene-transfected stromal cells. These results indicate that the highly branched N-glycosylation with augmented galactosylation is induced in human stromal cells immortalized by the telomerase expression.
authors.affiliation: Growth and Differentiation Research Group, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo 173-0015, Japan. furukawa@tmig.or.jp
authors.forename: Iku
authors.initials: I
authors.lastname: Kuwahara
authors.forename: Kenji
authors.initials: K
authors.lastname: Ikebuchi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hamada
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Niitsu
authors.forename: Keisuke
authors.initials: K
authors.lastname: Miyazawa
authors.forename: Kazuma
authors.initials: K
authors.lastname: Ohyashiki
authors.forename: Hirosuke
authors.initials: H
authors.lastname: Fujisawa
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Furukawa
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D048429:Cell Size; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D006031:Glycosylation; D006801:Humans; D037102:Lectins / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D004268:DNA-Binding Proteins; D037102:Lectins; D019098:Telomerase