Converting the sacrificial DNA repair protein N-ada into a catalytic methyl phosphotriester repair enzyme.
Mutation of the active-site residue Cys38 of N-Ada converts it from a sacrificial DNA repair protein to an enzyme that uses methanethiol as an external sacrificial reagent to repair DNA methyl phosphotriesters catalytically.
He
Chuan
C
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St., Cambridge, Massachusetts 02138, USA.
Wei
Hua
H
Verdine
Gregory L
GL
D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D002384:Catalysis; D011088:DNA Ligases / Q000737:chemistry* / Q000378:metabolism*; D004260:DNA Repair; D029968:Escherichia coli Proteins; D019853:O(6)-Methylguanine-DNA Methyltransferase; D010755:Organophosphates / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D014157:Transcription Factors
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D010755:Organophosphates; D014157:Transcription Factors; C045675:Ada protein, E coli; D019853:O(6)-Methylguanine-DNA Methyltransferase; D011088:DNA Ligases