[Improvement in the preparation of Paragonimus westermani chromosome for karyotype analysis].
OBJECTIVE
To improve Paragonimus westermani chromosome preparation technique.


METHODS
After being exposed to colchicine, the gonadal cells of P. westermani were treated by the following procedures: hypotonicity, fixation, dropping onto a slide and staining.


RESULTS
The chromosome number of P. westermani is 22, and the karyotype is 2m + 6Sm + 14t chromosome.


CONCLUSIONS
The improved technique of chromosome preparation is feasible to operate and the chromosome is clear enough for karyotype analysis.
Chen
Shao-hong
SH
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
Chang
Zheng-shan
ZS
Chen
Ming-gang
MG
Zhang
Yong-nian
YN
Feng
Zheng
Z
D000818:Animals; D002871:Chromosome Banding / Q000379:methods*; D002875:Chromosomes / Q000648:ultrastructure*; D004285:Dogs / Q000469:parasitology; D007621:Karyotyping; D010238:Paragonimus / Q000235:genetics* / Q000302:isolation & purification