title: Role for hydrogen peroxide in flow-induced dilation of human coronary arterioles.
abstract: Flow-induced dilation (FID) is dependent largely on hyperpolarization of vascular smooth muscle cells (VSMCs) in human coronary arterioles (HCA) from patients with coronary disease. Animal studies show that shear stress induces endothelial generation of hydrogen peroxide (H2O2), which is proposed as an endothelium-derived hyperpolarizing factor (EDHF). We tested the hypothesis that H2O2 contributes to FID in HCA. Arterioles (135+/-7 micro m, n=71) were dissected from human right atrial appendages at the time of cardiac surgery and cannulated with glass micropipettes. Changes in internal diameter and membrane potential of VSMCs to shear stress, H2O2, or to papaverine were recorded with videomicroscopy. In some vessels, endothelial H2O2 generation to shear stress was monitored directly using confocal microscopy with 2',7'-dichlorofluorescin diacetate (DCFH) or using electron microscopy with cerium chloride. Catalase inhibited FID (%max dilation; 66+/-8 versus 25+/-7%; P<0.05, n=6), whereas dilation to papaverine was unchanged. Shear stress immediately increased DCFH fluorescence in the endothelial cell layer, whereas treatment with catalase abolished the increase in fluorescence. Electron microscopy with cerium chloride revealed shear stress-induced increase in cerium deposition in intimal area surrounding endothelial cells. Exogenous H2O2 dilated (%max dilation; 97+/-1%, ED50; 3.0+/-0.7x10(-5) mol/L) and hyperpolarized HCA. Dilation to H2O2 was reduced by catalase, 40 mmol/L KCl, or charybdotoxin plus apamin, whereas endothelial denudation, deferoxamine, 1H-(1,2,4)-oxadiazole-[4,3-a]quinoxalin-1-one, or glibenclamide had no effect. These data provide evidence that shear stress induces endothelial release of H2O2 and are consistent with the idea that H2O2 is an EDHF that contributes to FID in HCA from patients with heart disease. The full text of this article is available at http://www.circresaha.org.
authors.affiliation: Department of Veterans Affairs Medical Center, Milwaukee, Wis 53226, USA. hmiura@mcw.edu
authors.forename: Hiroto
authors.initials: H
authors.lastname: Miura
authors.forename: John J
authors.initials: JJ
authors.lastname: Bosnjak
authors.forename: Gang
authors.initials: G
authors.lastname: Ning
authors.forename: Takashi
authors.initials: T
authors.lastname: Saito
authors.forename: Mamoru
authors.initials: M
authors.lastname: Miura
authors.forename: David D
authors.initials: DD
authors.lastname: Gutterman
mesh_terms: D001030:Apamin / Q000494:pharmacology; D001160:Arterioles / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D002374:Catalase / Q000494:pharmacology; D002563:Cerium; D003331:Coronary Vessels / Q000187:drug effects / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005456:Fluorescent Dyes; D005905:Glyburide / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism* / Q000494:pharmacology; D066298:In Vitro Techniques; D007502:Iron Chelating Agents / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D018715:Microscopy, Video; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D016877:Oxidants / Q000378:metabolism* / Q000494:pharmacology; D010208:Papaverine / Q000494:pharmacology; D026902:Potassium Channel Blockers / Q000494:pharmacology; D013314:Stress, Mechanical; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology*; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D007502:Iron Chelating Agents; D016877:Oxidants; D026902:Potassium Channel Blockers; D014665:Vasodilator Agents; D001030:Apamin; D002563:Cerium; D006861:Hydrogen Peroxide; D010208:Papaverine; D002374:Catalase; D005905:Glyburide