[Apoptosis of human pancreatic carcinoma cell lines induced by combinations of chemotherapeutic drugs].
OBJECTIVE
To investigate the apoptotic in human pancreatic carcinoma cell lines P3, SW1990 and Capan-2 induced by the combined treatment of 5-fluorouracil (5FU), mitomycin C (MMC), carboplatin(CBP) or adriamycine (ADM) or epirubicin (E-ADM).


METHODS
The changes of cell morphology were monitored by means of microscopic and fluorescence technique after treated with the combinations of chemotherapeutic drugs. The rate of apoptosis was calculated with flow cytometry and the fragmentation of DNA was measured by agarose gel electrophoresis.


RESULTS
The combinations of chemotherapeutic drugs were able to induce the apoptosis of human pancreatic carcinoma cell lines. Compared with the cell necrosis, the dosage of drugs was less and the time was shorter for inducing apoptosis. With the increased dosage and/or the time prolonged for treating the cells, the rate of apoptotic cells was increased. However, the apoptosis reaction of various human pancreatic carcinoma cell lines, which were mentioned above, induced by the same combinations of chemotherapeutic drugs were various different.


CONCLUSIONS
The apoptosis process might be a mechanism of an inhibiting cancer cell growth induced by the combinations of chemotherapeutic drugs. The examination of apoptosis reaction for the cells induced by different methods and/or chemotherapeutic drugs could become an important index of testing their ability of inhibiting pancreatic neoplasm.
Liao
Q
Q
Department of General Surgery, PUMC Hospital, CAMS and PUMC, Beijing, 100730.
Zhao
Y
Y
Cai
L
L
Wu
Y
Y
Zhu
Y
Y
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D016190:Carboplatin / Q000008:administration & dosage; D004317:Doxorubicin / Q000008:administration & dosage; D015251:Epirubicin / Q000008:administration & dosage; D005472:Fluorouracil / Q000008:administration & dosage; D006801:Humans; D016685:Mitomycin / Q000008:administration & dosage; D010190:Pancreatic Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D015251:Epirubicin; D016685:Mitomycin; D004317:Doxorubicin; D016190:Carboplatin; D005472:Fluorouracil