The preliminary study of interferon-gamma gene transfection to human Tenon's capsule fibroblasts in vitro.
OBJECTIVE
To investigate the results of the interferon-gamma (IFN-gamma) gene transfer and transient expression in human Tenon's capsule fibroblast in vitro in order to find a way to gene therapy in vivo.


METHODS
Using LipofectAMINE, IFN-gamma gene was transferred in human Tenon's capsule fibroblasts with plasmid pcDNA3 IFN-gamma. Its mRNA transcription and protein expression were determined by RT-PCR and flow cytometry assay respectively.


RESULTS
The human Tenon's capsule fibroblasts transferred the IFN-gamma gene can express the IFN-gamma in transcription and protein level transiently.


CONCLUSIONS
IFN-gamma gene can be transferred successfully and expressed expressed efficiently in human tenon's capsule fibroblast in vitro.
Lan
Y
Y
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Ge
J
J
Lin
M
M
Zheng
J
J
Chen
H
H
Liu
H
H
Wei
J
J
Li
Y
Y
D003228:Conjunctiva / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D018014:Gene Transfer Techniques; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D019076:Transgenes
D012333:RNA, Messenger; D007371:Interferon-gamma