title: YY1-DNA interaction results in a significant change of electronic context as measured by capacitance.
abstract: The detailed mechanism behind the processes of DNA-dependent RNA transcription initiation is largely unknown. When transcription initiation factors bind DNA, a significant change in the electrostatic state of the complex can result. Using electrical capacitance measurements of solutions of the YY1 zinc finger transcription initiation factor and the adeno-associated viral P5 promoter DNA, we observed a specific dielectric change when a protein-DNA complex was formed. We propose that complexation results in electrostatic changes that may trigger the markedly different electrical behavior, and offer a possible explanation for our results.
authors.affiliation: Harvard Medical School, Beth Israel Deaconess Medical Center, 99 Brookline Ave., Boston, MA 02215, USA.
authors.forename: Chu H
authors.initials: CH
authors.lastname: Choi
authors.forename: Joel
authors.initials: J
authors.lastname: Moser
authors.forename: Nicaulas A
authors.initials: NA
authors.lastname: Sabourin
authors.forename: Krastan
authors.initials: K
authors.lastname: Blagoev
authors.forename: Michael
authors.initials: M
authors.lastname: Naughton
authors.forename: Anny
authors.initials: A
authors.lastname: Usheva
authors.forename: Nicaulas
authors.initials: N
authors.lastname: Sobourin
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D025802:Electric Capacitance; D004583:Electrons; D050981:Erythroid-Specific DNA-Binding Factors; D006801:Humans; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011994:Recombinant Proteins; D055672:Static Electricity; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism; D050986:YY1 Transcription Factor
chemical_list: D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D011994:Recombinant Proteins; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C494698:YY1 protein, human; D004247:DNA