Bioluminescence assays: effects of quinones and phenols.
The influence of a series of quinones and phenols on bacteria bioluminescence systems was investigated. Three bioluminescence systems used in ecological monitoring were compared: (1) water-soluble; (2) immobilized in starch gel coupled enzyme systems: NADH:FMN-oxidoreductase-luciferase; (3) luminescent bacteria. Bioluminescence inhibition constants of quinones and phenols and bioluminescence induction periods were compared. These kinetic parameters are proportional to quinone concentrations and depend on the quinone redox potential. Different effects of the substances are related to structure and properties of the bioluminescence systems. The set of bioluminescence assays for quinones and phenols monitoring should include two bioluminescence systems: 1 (or 2) and 3.
Kudryasheva
N
N
Institute of Biophysics, Russian Academy of Sciences Siberian Branch, Krasnoyarsk, Russia. lucifer@ibp.krasnoyarsk.su
Vetrova
E
E
Kuznetsov
A
A
Kratasyuk
V
V
Stom
D
D
D004784:Environmental Monitoring / Q000379:methods*; D004800:Enzymes, Immobilized / Q000378:metabolism; D038181:FMN Reductase / Q000378:metabolism; D007700:Kinetics; D008156:Luciferases / Q000378:metabolism; D008163:Luminescent Measurements; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry / Q000633:toxicity*; D010776:Photobacterium / Q000187:drug effects / Q000201:enzymology; D011809:Quinones / Q000737:chemistry / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D004800:Enzymes, Immobilized; D010636:Phenols; D011809:Quinones; D014874:Water Pollutants, Chemical; D008156:Luciferases; D038181:FMN Reductase