Rapid identification of the genus fonsecaea by PCR with specific oligonucleotide primers.
An oligonucleotide primer set based on internal transcribed spacer regions of ribosomal DNA for PCR which gives the amplicon for only the DNA from Fonsecaea species was designed. This set yielded an amplicon with 333 bp for all strains of Fonsecaea pedrosoi and Fonsecaea compacta examined but no amplicons for related dematiaceous fungi and pathogenic yeasts. PCR using this primer set was considered to be a useful method for the rapid identification of the genus FONSECAEA:
Abliz
Paride
P
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan.
Fukushima
Kazutaka
K
Takizawa
Kayoko
K
Nieda
Norikazu
N
Miyaji
Makoto
M
Nishimura
Kazuko
K
D001203:Ascomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D021901:DNA, Intergenic / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis*; D006801:Humans; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D021901:DNA, Intergenic; D004275:DNA, Ribosomal