Aspiration method to collect epithelial cells from mouse, rat, and monkey oviducts.
When oviduct epithelial cells are collected by using enzymatic methods that involve protease such as trypsin, other cells unintentionally are collected as well, especially from small laboratory animals such as mice and rats, thus contaminating the collected sample. We therefore developed a simple nonenzymatic method that involves using a glass micropipette to aspirate the oviduct epithelial cells from the oviduct. This aspiration method easily removed oviduct epithelial cells from mice, rats, and cynomolgus monkeys. Culture of the collected oviduct epithelial cells confirmed that the cells could adhere and grow on a petri dish. Although this aspiration technique was developed for use in small animals, our results show that it can be applied to cynomolgus monkeys. The advantages of this technique are its simplicity and its success in collecting oviduct epithelial cells from oviducts.
Okada
Hironori
H
Tsukuba Primate Center for Medical Science, National Institute of Infectious Diseases, Hachimandai-1, Tsukuba, Ibaraki, 305-0843, Japan.
Hirose
Yoshihiro
Y
Ito
Masao
M
Sankai
Tadashi
T
D000818:Animals; D000830:Animals, Laboratory; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology*; D005187:Fallopian Tubes / Q000166:cytology*; D005260:Female; D008252:Macaca fascicularis; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary*; D013396:Suction / Q000379:methods / Q000662:veterinary*