Detection of fetal DNA in a cell pellet after centrifugation of mountant.
In order to obtain fetal cells (e.g., for paternity cases) after abortion, the centrifugation of mountant (in our case formalin) may be tried when the DNA examination of the fixed tissue itself gives limited or no (or not enough) information. The fixed tissue was microscopically negative for fetal cells and gave no satisfactory results when examined for DNA. Centrifugation of approximately 50 mL of reddish colored formalin resulted in a cell pellet that was examined for DNA, which gave enough information to confirm a case of sexual abuse.
Banaschak
Sibylle
S
Institute of Legal Medicine, Friedrich-Schiller-Universität Jena, Fürstengraben 23, D- 07743 Jena, Germany. Sibylle.Banaschak@med.uni-jena.de
Michael
Matthias
M
D000022:Abortion, Spontaneous; D000293:Adolescent; D000483:Alleles; D002498:Centrifugation; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods; D005260:Female; D005333:Fetus / Q000166:cytology*; D005557:Formaldehyde; D006801:Humans; D010334:Paternity; D011247:Pregnancy
D005557:Formaldehyde; D004247:DNA