[Sequences of 5S-rRNA gene spacer region and comparison of alkaloid content in Fritillaria thunbergii from different habitats].
OBJECTIVE
To study the genetic basis of the formation of indigenous Chinese medicine materials.


METHODS
The 5S-rRNA gene spacer regions in F. thunbergii from different habitats were amplified with AS and AS-1 as primers, and then sequenced. Total alkaloid contents were assayed by acid dye colorimetry, and 2 main alkaloid contents were assayed by pre-column derivatization and gas chromatographic method.


RESULTS
The sequenues of 5S-rRNA gene spacer regions in F. thunbergii from different habitats were same, and the length of them was 588 bp. They had same content total alkaloid. The results of gas chromatography showed that they had same kinds of monomer alkaloids, but the contents of different monomer alkaloids were different.


CONCLUSIONS
The difference of alkaloid content in F. thunbergii from various habitats isn't resulted from base sequence variation, but from microenvironment.
Cai
Z
Z
College of Traditional Chinese Medicine, Chinese Pharmaceutical University, Nanjing 210038.
Li
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P
Li
S
S
Dong
T
T
Zhan
H
H
D000470:Alkaloids / Q000032:analysis*; D002849:Chromatography, Gas; D021903:DNA, Ribosomal Spacer / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D017753:Ecosystem; D031410:Fritillaria / Q000737:chemistry / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D017423:Sequence Analysis, RNA
D000470:Alkaloids; D021903:DNA, Ribosomal Spacer; D004365:Drugs, Chinese Herbal; D012341:RNA, Ribosomal, 5S