Isolation, identification and localization of a second beetle antidiuretic peptide.
We isolated from head extracts of Tenebrio molitor a peptide that inhibits fluid secretion by the Malpighian tubules of this insect. This second antidiuretic factor, ADFb, like the previously published ADFa, works through cyclic GMP as a second messenger. It has primary structure Tyr-Asp-Asp-Gly-Ser-Tyr-Lys-Pro-His-Ile-Tyr-Gly-Phe-OH with an EC(50) of approximately 240 pM in a fluid secretion assay. This peptide is now the second sequenced endogenous insect ADF which inhibits Malpighian tubule fluid secretion. Immunohistochemical techniques show that the peptide is localized in the brain; it appears to be produced mainly in two pairs of bilaterally symmetrical cells in the protocerebrum.
Eigenheer
Richard A
RA
Department of Biochemistry, 160 Howard Medical Science, University of Nevada, Mail Stop 330, Reno, NV 89557-0014, USA.
Wiehart
Ursula M
UM
Nicolson
Susan W
SW
Schoofs
Liliane
L
Schegg
Kathleen M
KM
Hull
J Joe
JJ
Schooley
David A
DA
D000595:Amino Acid Sequence; D000818:Animals; D001517:Coleoptera; D004231:Diuresis / Q000187:drug effects*; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid
D010455:Peptides