title: Crystal structure of a MARCKS peptide containing the calmodulin-binding domain in complex with Ca2+-calmodulin.
abstract: The calmodulin-binding domain of myristoylated alanine-rich C kinase substrate (MARCKS), which interacts with various targets including calmodulin, actin and membrane lipids, has been suggested to function as a crosstalk point among several signal transduction pathways. We present here the crystal structure at 2 A resolution of a peptide consisting of the MARCKS calmodulin (CaM)-binding domain in complex with Ca2+-CaM. The domain assumes a flexible conformation, and the hydrophobic pocket of the calmodulin N-lobe, which is a common CaM-binding site observed in previously resolved Ca2+-CaM-target peptide complexes, is not involved in the interaction. The present structure presents a novel target-recognition mode of calmodulin and provides insight into the structural basis of the flexible interaction module of MARCKS.
authors.affiliation: Harima Institute at SPring-8, RIKEN, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
authors.forename: Emiko
authors.initials: E
authors.lastname: Yamauchi
authors.forename: Toru
authors.initials: T
authors.lastname: Nakatsu
authors.forename: Mamoru
authors.initials: M
authors.lastname: Matsubara
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kato
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Taniguchi
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002118:Calcium / Q000737:chemistry*; D002147:Calmodulin / Q000737:chemistry* / Q000378:metabolism*; D018360:Crystallography, X-Ray; D057927:Hydrophobic and Hydrophilic Interactions; D047908:Intracellular Signaling Peptides and Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary
chemical_list: D002147:Calmodulin; D047908:Intracellular Signaling Peptides and Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D010455:Peptides; D010750:Phosphoproteins; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D002118:Calcium