[Anti-Toxoplasma effect of activated mouse macrophages induced by interferon-gamma combined with TNF-alpha].
OBJECTIVE
To compare the inhibitory effect of the macrophages activated by IFN-gamma combined with TNF-alpha against RH strain and Fukaya strain.


METHODS
The average parasite proliferation rates of the two strains within the cytokine-activated M phi s were calculated at different times post-challenge, the nitric oxide (NO) levels in the medium supernatant were simultaneously determined.


RESULTS
In the macrophages activated by 100 U each of IFN-gamma and TNF-alpha, the invaded tachyzoites of RH strain were completely killed, while the invaded tachyzoites of Fukaya strain remained slow proliferation with significantly lower levels of NO detected at 24 h post challenge.


CONCLUSIONS
The difference in the anti-Toxoplasma effect of the activated macrophages against RH and Fukaya strains might be attributed to the different amount of NO produced by the macrophages.
Zhang
A
A
Department of Parasitology, Medical School, Shanghai Tiedao University, Shanghai 200331.
Yang
H
H
Yang
Y
Y
Qian
Z
Z
D000818:Animals; D002478:Cells, Cultured; D004357:Drug Synergism; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology*; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D014122:Toxoplasma / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D007371:Interferon-gamma