[Association expression of genes encoding gst of Schistosoma japonicum and enterotoxigenic Escherichia coli].
OBJECTIVE
To study the association expression of two genes encoding GST of Schistosoma japonicum and K99 of enterotoxigenic Escherichia coli.


METHODS
PCR technique was used to gain the K99 gene. After digestion with BamH I and EcoR I, the gene was cloned into the plasmid vector pUC18 by using recombinant DNA techniques. The other target gene of GST in pSj5 plasmid was obtained by EcoR I digestion, and was then ligated into the recombinant plasmid pUC18-K99. The expressed product was assayed by SDS-PAGE, Western blotting, reversal IHA, ELISA and transmission electron microscopy.


RESULTS
Co-expression product of around 50 kDa was obtained. The protein was recognized by anti-GST and anti-K99 antibodies (ELISA and IHA), and acted as pilus on the surface of transformed DH5 alpha E. coli bacteria.


CONCLUSIONS
Co-expression of the genes encoding GST and K99 was successfully achieved.
Zhang
W
W
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Zhang
J
J
Liu
S
S
D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology; D000954:Antigens, Surface / Q000235:genetics* / Q000276:immunology; D001427:Bacterial Toxins / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology*; D010861:Fimbriae, Bacterial / Q000276:immunology; D015870:Gene Expression; D005982:Glutathione Transferase / Q000235:genetics* / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D012549:Schistosoma japonicum / Q000201:enzymology* / Q000235:genetics / Q000276:immunology
D000947:Antigens, Helminth; D000954:Antigens, Surface; D001427:Bacterial Toxins; C031429:K99 antigen; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase