Kinetics of T cell-activation molecules in response to Mycobacterium tuberculosis antigens.
The phenotypic features acquired subsequent to antigen-specific stimulation in vitro were evaluated by means of the kinetic expressions of CD69 and CD25 activation molecules on T lymphocytes and assayed by flow cytometry in response to PPD, Ag85B, and ferritin in PPD-positive healthy control individuals. In response to PHA, CD69 staining on both CD4+ and CD8+ T cells became initially marked after 4 h, peaked at 24 h, and quickly decreased after 120 h. For CD25, a latter expression was detected around 8 h, having increased after 96 h. As expected, the response rate to the mycobacterial antigens was much lower than that to the mitogen. Positive staining was high after 96 h for CD25 and after 24 h for CD69. CD69 expression was significantly enhanced (p < 0.05) on CD8+ as compared to CD4+ T cells. High levels were also found between 96-120 h. Regarding Ag85B, CD25+ cells were mostly CD4+ instead of CD8+ T cells. Moreover, in response to ferritin, a lower CD25 expression was noted. The present data will allow further characterization of the immune response to new mycobacterial-specific antigens and their evaluation for possible inclusion in developing new diagnostic techniques for tuberculosis as well in a new vaccine to prevent the disease.
Antas
Paulo R Z
PR
Laboratório de Hanseníase, Instituto Oswaldo Cruz, Fiocruz, 21045-900 Rio de Janeiro, RJ, Brasil. pzuquim@ioc.fiocruz.br
Oliveira
Eliane B
EB
Milagres
Alexandre S
AS
Franken
Kees C
KC
Ottenhoff
Tom H M
TH
Klatser
Paul
P
Sarno
Euzenir N
EN
Sampaio
Elizabeth P
EP
D000217:Acyltransferases / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D015703:Antigens, CD / Q000032:analysis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D001426:Bacterial Proteins / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005293:Ferritins / Q000276:immunology; D005434:Flow Cytometry; D037181:Lectins, C-Type; D008213:Lymphocyte Activation / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000032:analysis*; D014373:Tuberculin / Q000276:immunology; D000099192:CD69 Antigens
D000217:Acyltransferases; D000942:Antigens, Bacterial; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D001426:Bacterial Proteins; D005293:Ferritins; D037181:Lectins, C-Type; D015375:Receptors, Interleukin-2; D014373:Tuberculin; D000099192:CD69 Antigens; C121433:antigen 85B, Mycobacterium tuberculosis