[Obtaining and construction of a novel gene from Schistosoma japonicum by expressed sequence tags].
OBJECTIVE
To look for new candidate vaccine molecule and characterize new genes from Schistosoma japonicum.


METHODS
New genes of S. japonicum were obtained through expressed sequence tags and analyzed on line. One interest cDNA was cloned into anti-sense eukaryotic expression vector.


RESULTS
An interest cDNA JAYL0230 was obtained. Analysis showed that homology existed between its deduced amino acid sequence and defender against apoptotic death 1 of human and pig, etc. Its anti-sense cDNA was cloned into eukaryotic expression vector pEGFP-N3.


CONCLUSIONS
The combination of expressed sequence tags and bioinformatics is an effective way to find novel genes of creature. Construction of anti-sense eukaryotic vector is helpful in studying gene's function.
Li
Yan
Y
Department of Parasitology, Sun Yat-Sen University of Medical Sciences, Guangzhou 510080.
Yu
Xin-bing
XB
Wu
Zhong-dao
ZD
Li
Hui-ting
HT
Bao
Jun-ying
JY
Shao
Xiao
X
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry; D020224:Expressed Sequence Tags; D008969:Molecular Sequence Data; D012549:Schistosoma japonicum / Q000235:genetics*
D018076:DNA, Complementary