[Anti-leukemia effect of sophora flavescens combined with the low molecular weight natural tumor suppressor of the human fetal liver and its mechanism].
OBJECTIVE
To explore the effect of sophora flavescens (SF) combined low molecular weight natural tumor suppressor(LMW-NTS) on normal human bone marrow CFU-GM and acute myelogenous leukemia cells and its mechanism.


METHODS
CFU-GM colony culture, cell culture, Wrigh-Giemsa stain, 3H-TdR incorporation test, agarose gel electrophoresis of DNA fragment and RT-PCR were employed in this research.


RESULTS
The combination of SF and LMW-NTS greatly inhibited the proliferation of acute meylogenous leukemia cells, induced their apoptosis, and down-regulated the c-Myc expression as compared with SF or LMW-NTS alone.


CONCLUSIONS
The anti-leukemia effect of the combination of SF and LMW-NTS is superior to SF or LMW-NTS alone.
Xiang
Qiu
Q
Laboratory of Blood Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Tan
Meng-qun
MQ
Huang
Yan-hong
YH
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005333:Fetus; D018922:HL-60 Cells / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D008099:Liver / Q000737:chemistry; D008970:Molecular Weight; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics; D029909:Sophora; D014020:Tissue Extracts / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D016271:Proto-Oncogene Proteins c-myc; D014020:Tissue Extracts