[Study on inhibitory effect of ampelopsin on melanoma by serologic pharmacological method].
OBJECTIVE
To study the inhibitory effect of ampelopsin on melanoma.


METHODS
Using a serologic pharmacological method, the inhibitory effects of different dose of ampelopsin on B16 melanoma cells were evaluated by a MTT assay and a flow cytometry.


RESULTS
The serums of C57BL/6 mices, which were administered by 0.420 mmol/kg dose and 0.560 mmol/kg dose of ampelopsin, could obviously suppress the B16 melanoma cells, and the inhibitory rate was equal to that of the vacant serum adding same consistency ampelopsin. The drug serums of the two doses groups reduced the cleavage and proliferation index of B16 melanoma cells to 19.1% and 21.7% respectively.


CONCLUSIONS
Ampelopsin possibly acts on melanoma B16 cells by original form drug at the period with a peak of the drug concentration, and inhibits the DNA synthesis of B16 cells.
Liu
D
D
Sun Yat-sen University of Medical Sciences, GuangZhou 510089.
Luo
M
M
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000627:therapeutic use*; D008546:Melanoma, Experimental / Q000188:drug therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D019384:Nucleic Acid Synthesis Inhibitors / Q000627:therapeutic use
D000970:Antineoplastic Agents; D005419:Flavonoids; D019384:Nucleic Acid Synthesis Inhibitors; C106407:ampelopsin