[The effect of antioxidants on the in vitro life-span of keratinocyte].
The effect of antioxidants on the in vitro life span of mouse keratinocytes was investigated in this work. It was found that the life span of the keratinocytes cultured in the medium supplemented with antioxidants was extended significantly. The most beneficial antioxidant used in this work was the mercaptoethanol, followed by the catalase and SOD. However, the growth rates of keratinocytes in vitro under all the experimental conditions still declined with the culture time. It was also found that the antioxidants added in the medium were also helpful to enhance the keratinocyte colony formation. In addition, the aging kinetics of the mouse epidermal keratinocytes in vitro were analyzed, and finally the aging rate constants corresponding to antioxidants used were calculated.
Zhou
Yan
Y
State Key Laboratory of Bioreactor Engineering, ECUST, Shanghai 200237, China.
Ouyang
An-Li
AL
Hua
Ping
P
Tan
Wen-Song
WS
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects*; D008623:Mercaptoethanol / Q000494:pharmacology; D051379:Mice; D013482:Superoxide Dismutase / Q000494:pharmacology
D000975:Antioxidants; D008623:Mercaptoethanol; D002374:Catalase; D013482:Superoxide Dismutase