[Sequence difference of ribosomal DNA second internal transcribed spacer in Anopheles minimus in different localities].
OBJECTIVE
To compare the sequence difference of the ribosomal DNA second internal transcribed spacer (ITS2) in Anopheles minimus from Yunnan, Hainan, Guangxi of China and from Thailand.


METHODS
Single mosquito's legs were digested to extract DNA; the rDNA-ITS2 region was amplified by specific primers and the PCR products were purified, sequenced and analysed.


RESULTS
Two different kinds of ITS2 sequences, temporarily named as An. minimus A and An. minimus C, were found in this study. The length and GC contents of ITS2 region in species A and species C are 481 bp, 54.04% and 483 bp, 54.23%, respectively. Two species differed by 5.8% in the ITS2 sequences owing to 22 fixed nucleotide substitutions and indels.


CONCLUSIONS
Two cryptic species, An. minimus A and An. minimus C, were found in the research localities.
Zhou
Shui-sen
SS
Institute for Parasitic Disease Control and Prevention, Chinese Center for Disease Control and Prenention, Shanghai 200025.
Tang
Lin-hua
LH
Gu
Zheng-cheng
ZC
Wang
Yi
Y
D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000737:chemistry*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry*; D008969:Molecular Sequence Data
D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer