title: Trypan blue not toxic for retinal pigment epithelium in vitro.
abstract: OBJECTIVE
  To investigate whether trypan blue has a toxic effect on cultured retinal pigment epithelial (retinal pigment epithelium) cells.
  
  
  METHODS
  Experimental study with a direct live/dead cell staining technique using fluorescent dyes.
  
  
  METHODS
  Cultured human retinal pigment epithelium cells were exposed for 5 minutes to various concentrations of trypan blue (0.06%, 0.15%, 0.30%), and cell viability was confocally measured.
  
  
  RESULTS
  No increased cell death was found in cultures incubated in any of the trypan blue concentrations used.
  
  
  CONCLUSIONS
  These findings indicate that a short exposure of trypan blue does not have a toxic effect on cultured retinal pigment epithelium cells.
authors.affiliation: Department of Ophthalmology, UZLeuven, Leuven, Belgium. Peter.Stalmans@uz.kuleuven.ac.be
authors.forename: Peter
authors.initials: P
authors.lastname: Stalmans
authors.forename: Elisabeth H
authors.initials: EH
authors.lastname: Van Aken
authors.forename: Gerrit
authors.initials: G
authors.lastname: Melles
authors.forename: Marc
authors.initials: M
authors.lastname: Veckeneer
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Feron
authors.forename: Ingeborg
authors.initials: I
authors.lastname: Stalmans
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents / Q000494:pharmacology*; D006801:Humans; D018613:Microscopy, Confocal; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000187:drug effects*; D014343:Trypan Blue / Q000494:pharmacology*
chemical_list: D004396:Coloring Agents; D014343:Trypan Blue