[Effect of pyronaridine, mefloquine and quinine on artesunate-sensitive and artesunate-resistant Plasmodium falciparum].
OBJECTIVE
To compare the sensitivity of artesunate-sensitive and artesunate-resistant P. falciparum to pyronaridine, mefloquine and quinine and to understand the effect of artesunte combined with the above-mentioned 3 drugs respectively on artesunate-resistant P. falciparum.


METHODS
Rieckmann's in vitro miorotechnique was used.


RESULTS
The ID50 values of pyronaride, mefloquine, quinine and artesunate were 59.0, 69.7, 283.8 and 9.6 nmol/L to artesunate-sensitive P. falciparum; the ID50 of the 4 drugs mentioned above were 170.6, 63.2, 272.4 and 85.1 nmol/L to the artesunate-resistant P. falciparum, respectively. In artesunate pyronaridine combination, the ID50 values were 1/3.7 (22.8/85.1) and 1/4.7 (36.6/170.6) of the 2 drugs singly used. In artesunate mefloquine combinaton, the ID95 is 1/125 (3.2/400) and 1/16 (80/128) of the 2 drugs singly used, respectively.


CONCLUSIONS
The artesunate-resistant P. falciparum isolate has no cross resistance to mefloquine and quinine. When artesunate was used in combination with the 2 drugs mentioned above respectively, the efficacy proved to be enhanced.
Yang
H L
HL
Yunnan Institute of Malaria Control, Simao 665000.
Gao
B H
BH
Huang
K G
KG
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D037621:Artemisinins / Q000494:pharmacology; D000077332:Artesunate; D004351:Drug Resistance; D004357:Drug Synergism; D015767:Mefloquine / Q000494:pharmacology; D009287:Naphthyridines / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000187:drug effects*; D011803:Quinine / Q000494:pharmacology; D012717:Sesquiterpenes / Q000494:pharmacology
D000962:Antimalarials; D037621:Artemisinins; D009287:Naphthyridines; D012717:Sesquiterpenes; D000077332:Artesunate; D011803:Quinine; C027871:pyronaridine; D015767:Mefloquine