[Capillary zone electrophoresis for determination of thymulin in human plasma].
OBJECTIVE
To establish a capillary zone electrophoresis (CZE) method for determination of thymulin in human blood plasma. Thymulin is a nonapeptide produced by the thymic epithelium.


METHODS
The uncoated capillary used was 75 microns ID x 57 cm (50 cm effective length). The experimental conditions were as follows: the running buffer was phosphate-borate buffer containing 50 mmol.L-1 Na2B4O7-10 mmol.L-1 Na2HPO4 (pH 9.10); running voltage was 12 kV; operated temperature was 25 degrees C; running time was 13 minutes, detective wavelength was 200 nm.


RESULTS
The linear range of the thymulin standard curve was 1-10 micrograms.mL-1, gamma = 0.9990, the average recovery was 70.97% with RSD of 3.32%, the RSD of reproducibility was 3.05%.


CONCLUSIONS
The method of CZE for determination of thymulin in human plasma is simple and reliable.
Chen
Jing
J
Insitute of Nutrition and Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China.
Qu
Ning
N
Xia
Yi-ming
YM
D002675:Child, Preschool; D019075:Electrophoresis, Capillary; D006801:Humans; D010949:Plasma / Q000737:chemistry*; D013935:Thymic Factor, Circulating / Q000032:analysis*
D013935:Thymic Factor, Circulating