title: [Establishment and identification of the monoepitopic monoclonal antibody of Plasmodium falciparum].
abstract: OBJECTIVE
  To prepare a monoepitopic monclonal antibody of Plasmoduim falciparum.
  
  
  METHODS
  According to the theory of protein structure, a peptide with 6-9 residues representting the antigenicity of the original histidine-rich protein II (HRP-II) of P. falciparum was synthesized and used to immunize BALB/c mice after purity identification by capillary electrophoresis.
  
  
  RESULTS
  Four hybridoma cell lines targeting a suitable peptide from pf HRP-II were obtained by using spleen embedment method and hybridoma technology.
  
  
  CONCLUSIONS
  It is the first report to prepare a monoepitopic monoclonal antibody against original protein by selecting a suitable peptide from the primary sequence of protein.
authors.affiliation: Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
authors.forename: J
authors.initials: J
authors.lastname: Qu
authors.forename: B
authors.initials: B
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Bao
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: X
authors.initials: X
authors.lastname: Xu
authors.forename: P
authors.initials: P
authors.lastname: Fan
authors.forename: Y
authors.initials: Y
authors.lastname: Ren
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002460:Cell Line; D006825:Hybridomas / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010963:Plasmodium falciparum / Q000276:immunology*; D011506:Proteins / Q000138:chemical synthesis / Q000276:immunology*; D000099053:Histidine-Rich Glycoprotein
chemical_list: D000911:Antibodies, Monoclonal; D011506:Proteins; D000099053:Histidine-Rich Glycoprotein