[Physico-chemical properties and biological activities of a glycoconjugate SPPA-1 from Spirulina platensis].
OBJECTIVE
To isolate polysaccharide from Spirulina platensis and determine its sugar position, molecular weight and biological activities.


METHODS
Sephadex G-75 and CM-Sephadex C-50 were used. The sugar position was analyzed by gas chromatography, the molecular weight was determined by GPC. The homogeneity of this glycoconjugate was determined by HPLC and CE. IR and NMR spectra were used to determine the glycosidic linkage.


RESULTS
SPPA-1 was a homogeneous glycoconjugate, its carbohydrate chain was composed of alpha-glucopyranan, carbohydrate content is 91.70%, Nitrogen content was 0.96%, the molecular weight was 69.00 x 10(4), SPPA-1 could eliminate O2-. radicals.


CONCLUSIONS
SPPA-1 was an antioxidative glycoconjugate from Spirulina platensis.
Wang
Z F
ZF
Center of Biological Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Peng
Z Y
ZY
Huang
L J
LJ
Lu
R
R
Tian
G Y
GY
D000596:Amino Acids / Q000032:analysis; D000458:Cyanobacteria / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006001:Glycoconjugates / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008970:Molecular Weight; D010100:Oxygen / Q000378:metabolism; D011134:Polysaccharides / Q000302:isolation & purification
D000596:Amino Acids; D016166:Free Radical Scavengers; D006001:Glycoconjugates; D011134:Polysaccharides; D010100:Oxygen