[The expression of collagen type I and type II during the second fracture healing].
OBJECTIVE
This study was conducted to disclose the rules of collagen type I and II production and hence to acquire knowledge about the mechanism of repairing during the second period of fracture healing.


METHODS
An experimental model of second fracture healing at rat's femurs was used to study the production of collagen type I and II by means of immunohistochemistry; meanwhile the histologic changes were observed with the use of HE staining and Alcian blue staining at 3 days, 1, 2, 3, 4 weeks postoperatively.


RESULTS
The expression of both collagen type I and II was detected by immunohistochemistry at 1 week. The peak expression of collagen type II occurred at 3 weeks. The higher expression of collagen type I was observed at 4 weeks.


CONCLUSIONS
The morphologic changes of chondrocyte and the production of collagen type I and II by chondrocyte in cartilage callus are correlated with their repairing function during different periods of fracture healing.
Zhang
Xian
X
Department of Anatomy, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Wang
Fan
F
Jiang
Hongmei
H
Li
Ruixiang
R
D000818:Animals; D002146:Bony Callus / Q000737:chemistry; D024042:Collagen Type I / Q000032:analysis*; D024043:Collagen Type II / Q000032:analysis*; D005264:Femoral Fractures / Q000378:metabolism* / Q000503:physiopathology; D017102:Fracture Healing; D008297:Male; D051381:Rats
D024042:Collagen Type I; D024043:Collagen Type II