[Cellular immune response in mice vaccinated with uv-attenuated Toxoplasma gondii ZS1 strain trophozoites].
OBJECTIVE
To explore the protective effect and cellular immune response of uv-attenuated ZS1 strain trophozoites of Toxoplasma gondii in mice.


METHODS
The ZS1 strain trophozoites of T. gondii were irradiated by uv-light with 2537 A wave length for 60 minutes. Mice were divided into 4 groups. Group 1 was vaccinated alone, group 2 was challenged with normal ZS1 trophozoites on d45 after vaccination, group 3 was infected alone, and group 4 was normal control. The changes in splenic T cell proliferation, level of CD4+ and CD8+ T cell, and NK cell activity were compared.


RESULTS
Group 1 mice survived normally, no trophozoite, pseudo-cyst or cyst was detected in the tissues on d49 after vaccination. Group 2 mice survived longer than those of group 3. The T lymphocyte proliferation in response to soluble antigen of T. gondii was significantly enhanced in group 2, and suppressed in group 3. The level of CD4+ T cell in group 2 was decreased, resulting in a reverse of CD4+/CD8+ ratio. The NK cell activities in groups 1, 2 and 3 were all significantly increased.


CONCLUSIONS
The uv-attenuated vaccine of T. gondii ZS1 strain could induce certain protective immunity against challenge infection, in which CD8+ T cell and NK cell might play an important role.
Lu
F
F
Department of Parasitology, Sun Yat-Sen University of Medical Sciences, Guangzhou 510089.
Zheng
H
H
Guo
H
H
Chen
G
G
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D007111:Immunity, Cellular; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D013154:Spleen / Q000166:cytology; D014122:Toxoplasma / Q000276:immunology* / Q000528:radiation effects; D014124:Toxoplasmosis, Animal / Q000276:immunology*; D014466:Ultraviolet Rays; D014613:Vaccines, Attenuated / Q000276:immunology*
D014613:Vaccines, Attenuated