title: Genetic evidence for two sibling species within Contracaecum ogmorhini Johnston & Mawson, 1941 (Nematoda: Anisakidae) from otariid seals of boreal and austral regions.
abstract: Genetic variation of Contracaecum ogmorhini (sensu lato) populations from different otariid seals of the northern and southern hemisphere was studied on the basis of 18 enzyme loci as well as preliminary sequence analysis of the mitochondrial cyt b gene (260 bp). Samples were collected from Zalophus californianus in the boreal region and from Arctocephalus pusillus pusillus, A. pusillus doriferus and A. australis from the austral region. Marked genetic heterogeneity was found between C. ogmorhini (sensu lato) samples from the boreal and austral region, respectively. Two loci (Mdh-2 and NADHdh) showed fixed differences and a further three loci (Iddh, Mdh-1 and 6Pgdh) were highly differentiated between boreal and austral samples. Their average genetic distance was D(Nei) = 0.36 at isozyme level. At mitochondrial DNA level, an average proportion of nucleotide substitution of 3.7% was observed. These findings support the existence of two distinct sibling species, for which the names C. ogmorhini (sensu stricto) and C. margolisi n. sp., respectively, for the austral and boreal taxon, are proposed. A description for C. margolisi n. sp. is provided. No diagnostic morphological characters have so far been detected; on the other hand, two enzyme loci, Mdh-2 and NADHdh, fully diagnostic between the two species, can be used for the routine identification of males, females and larval stages. Mirounga leonina was found to host C. ogmorhini (s.s.) in mixed infections with C. osculatum (s.l.) (of which C. ogmorhini (s.l.) was in the past considered to be a synonym) and C. miroungae; no hybrid genotypes were found, confirming the reproductive isolation of these three anisakid species. The hosts and geographical range so far recorded for C. margolisi n. sp. and C. ogmorhini (s.s.) are given.
authors.affiliation: Department of Public Health Sciences, Section of Parasitology, University of Rome La Sapienza, P.le Aldo Moro, 5, 00185 Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Mattiucci
authors.forename: R
authors.initials: R
authors.lastname: Cianchi
authors.forename: G
authors.initials: G
authors.lastname: Nascetti
authors.forename: L
authors.initials: L
authors.lastname: Paggi
authors.forename: N
authors.initials: N
authors.lastname: Sardella
authors.forename: J
authors.initials: J
authors.lastname: Timi
authors.forename: S C
authors.initials: SC
authors.lastname: Webb
authors.forename: R
authors.initials: R
authors.lastname: Bastida
authors.forename: D
authors.initials: D
authors.lastname: Rodríguez
authors.forename: L
authors.initials: L
authors.lastname: Bullini
mesh_terms: D000818:Animals; D001267:Atlantic Ocean; D001483:Base Sequence; D003573:Cytochrome b Group / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D004592:Electrophoresis, Starch Gel / Q000662:veterinary; D005260:Female; D014644:Genetic Variation; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009348:Nematoda / Q000033:anatomy & histology / Q000201:enzymology* / Q000235:genetics*; D010140:Pacific Ocean; D046048:Seals, Earless / Q000469:parasitology*; D016415:Sequence Alignment
chemical_list: D003573:Cytochrome b Group; D004272:DNA, Mitochondrial; D007527:Isoenzymes