Simple sequence repeat (SSR) analysis for assessment of genetic variability in apricot germplasm.
Thirty SSR primer combinations, developed from peach SSR-enriched genomic libraries and BAC libraries of peach [ Prunus persica (L.) Batsch.], were tested for cross amplification with 74 apricot ( Prunus armeniaca L.) germplasm accessions. Twelve primer pairs amplified 14 polymorphic SSR loci useful for discriminating most apricot cultivars, as well as for investigating patterns of variation in apricot germplasm. Levels of polymorphism were higher than the levels described using other codominant marker systems (i.e., isozymes, RFLP markers). Overall, 107 alleles were identified, and all but 11 accessions were unambiguously discriminated. Genetic differentiation of native germplasm into traditional ecogeographical groups was low, with a high level of genetic identity (> 0.75) between the groups. However, neighbor joining cluster analysis of marker distances between cultivars reflected the complex history of apricot domestication, producing groupings not evidently based on the geographical origin of the cultivars. Distant positioning of Chinese cultivars on UPGMA and neighbor joining dendrograms supports the authors' consideration of Chinese apricots as subspecies, Prunus armeniaca var. ansu Maxim., rather than a separate species.
Zhebentyayeva
T N
TN
Department of Horticulture, Clemson University, Clemson SC 29634, USA. tzhebe@clemson.edu
Reighard
G L
GL
Gorina
V M
VM
Abbott
A G
AG
D000483:Alleles; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D017931:DNA Primers; D018744:DNA, Plant / Q000032:analysis; D004463:Ecology; D015723:Gene Library; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D018895:Microsatellite Repeats; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D027861:Prunus / Q000235:genetics*; D020080:Tandem Repeat Sequences
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers