Microsatellite analysis of chromosome 3p region in sporadic renal cell carcinomas.
The etiology and progression of renal carcinomas (RCC) is still poorly understood. RCC have been classified into several pathological entities. The most frequent type, clear cell carcinoma, accounts for about 80% of sporadic RCC and shows several chromosome abnormalities documented both by conventional cytogenetics, loss of eterozygosity (LOH) and replication error (RER) studies. In 10 clear cell type sporadic RCC we evaluated LOH and RER using a set of 10 microsatellite markers covering the chromosome 3p region, which has been suggested for interstitial deletions. Electrophoresis was performed by automated sequencer ABI Prism 377 and data were analyzed with Genescan and Genotyper 2.5 softwares. We revealed allelic loss in 48,7% of informative microsatellites and a single case of RER. We found the highest LOH frequency in 3p25-26 region where maps Von Hippel-Lindau (VHL) oncosuppressor gene. In addition, DNA hypermethylation, an alternative mechanism of VHL gene silencing, was evaluated by methylation-specific PCR. However hypermethylation status was not detected in any of our tumor samples.
Girolami
Francesca
F
Cytogenetics and Genetics Unit, Azienda Ospedaliera Careggi and II Urological Clinic, Firenze, 50134, Italy. citogenbibl@ao-careggi.toscana.it
Passerini
Ilaria
I
Gargano
Dorotea
D
Frusconi
Sabrina
S
Villari
Donata
D
Nicita
Giulio
G
Torricelli
Francesca
F
D001483:Base Sequence; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D017931:DNA Primers; D004261:DNA Replication / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats
D017931:DNA Primers; D004273:DNA, Neoplasm