Homocysteine inhibits the proliferation and invasive potential of HT-1080 human fibrosarcoma cells.
The impairment of homocysteine metabolism has been related to several disorders and diseases. Recently, homocysteine has been shown to inhibit key steps of angiogenesis, including endothelial cell proliferation, invasion, and remodeling of the extracellular matrix. Since these are also key steps in tumor invasion and metastasis, it can be hypothesized that homocysteine can also interfere in these processes. Therefore, we studied the effects of homocysteine on tumor proliferation and invasion, as well as on urokinase, a key extracellular matrix-degrading protease, using a model human tumor cell line. This study demonstrates that, in fact, homocysteine inhibits HT-1080 proliferation and invasion, and is a potent inhibitor of tumor cell urokinase expression.
Chavarría
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Department of Molecular Biology and Biochemistry, Faculty of Sciences, University of Málaga, E-29071 Málaga, Spain.
Sánchez-Jiménez
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Quesada
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Medina
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D002455:Cell Division / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D003094:Collagen; D016895:Culture Media, Serum-Free; D004338:Drug Combinations; D005354:Fibrosarcoma / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D006710:Homocysteine / Q000494:pharmacology*; D006801:Humans; D007797:Laminin; D009361:Neoplasm Invasiveness; D011509:Proteoglycans; D014407:Tumor Cells, Cultured; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*
D016895:Culture Media, Serum-Free; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D006710:Homocysteine; C059485:matrigel; D003094:Collagen; D014568:Urokinase-Type Plasminogen Activator