[Circulating antigen detection in schistosomiasis japonica patients by sandwich ELISA using polyclonal and monoclonal antibodies].
OBJECTIVE
To detect circulating antigen in the patients with schistosomiasis.


METHODS
Polyclonal antibody(PcAb)-monoclonal antibody(McAb) sandwich ELISA.


RESULTS
Sera from 150 patients with schistosomiasis were detected, the average sensitivity was 84.7%(72.0%-91.0%). No false positive reaction was detected in 40 normal controls and none but 2 patients(2/10) with paragonimiasis showed cross reactions among 74 cases with other parasitic infections.


CONCLUSIONS
PcAb-McAb sandwich ELISA is a relatively ideal method for circulatling antigen detection.
Qiu
L S
LS
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Feng
Z
Z
Zhang
Y H
YH
Li
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis / Q000276:immunology*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth