title: Cloning and characterization of a jasmonic acid-responsive gene encoding 12-oxophytodienoic acid reductase in suspension-cultured rice cells.
abstract: In suspension-cultured rice ( Oryza sativaL.) cells, jasmonic acid (JA) functions as a signal transducer in elicitor N-acetylchitoheptaose-induced phytoalexin production. Differential screening of a cDNA library constructed using poly(A)(+) RNA from suspension-cultured rice cells treated with JA (10(-4) M) for 2 h yielded a cDNA for a gene that responded to exogenous JA by an increase in mRNA level. Nucleotide sequence analysis indicated that the cDNA encodes an homologue of the yeast Old Yellow Enzyme. The deduced amino acid sequence was very similar to the sequences of 12-oxophytodienoic acid reductases (OPR) 1 and 2 from Arabidopsis thaliana(AtOPR1 and AtOPR2) and OPR1 from tomato ( Lycopersicon esculentum) (LeOPR1). The cDNA-encoded protein purified from recombinant Escherichia coli cells as a hexahistidine-tagged fusion protein exhibited OPR activity similar to that of AtOPR1, AtOPR2, and LeOPR1, which catalyze reduction of (-)- cis-12-oxophytodienoic acid (OPDA) preferentially over (+)- cis-OPDA, a natural precursor of JA. Thus the rice enzyme was termed OsOPR1. The physiological roles of OsOPR1 are discussed. This is the first report of the cloning of an OPR gene from a monocot plant.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sobajima
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Takeda
authors.forename: Miho
authors.initials: M
authors.lastname: Sugimori
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kobashi
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kiribuchi
authors.forename: Eun-Min
authors.initials: EM
authors.lastname: Cho
authors.forename: Chiharu
authors.initials: C
authors.lastname: Akimoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Eiichi
authors.initials: E
authors.lastname: Minami
authors.forename: Naoto
authors.initials: N
authors.lastname: Shibuya
authors.forename: Florian
authors.initials: F
authors.lastname: Schaller
authors.forename: Elmar W
authors.initials: EW
authors.lastname: Weiler
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Yoshihara
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nishida
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nojiri
authors.forename: Toshio
authors.initials: T
authors.lastname: Omori
authors.forename: Makoto
authors.initials: M
authors.lastname: Nishiyama
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Yamane
mesh_terms: D002478:Cells, Cultured; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D012275:Oryza / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors; D054883:Oxylipins; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D003517:Cyclopentanes; D018076:DNA, Complementary; D005231:Fatty Acids, Unsaturated; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; C025999:12-oxophytodienoic acid; C011006:jasmonic acid; D003513:Cycloheximide; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C106360:12-oxophytodienoate reductase