[Determination of ochratoxin A by ELISA I. Study on the preparation of ochratoxin a antigen].
Ochratoxin A (OTA) antigen was prepared by activated ester method. Factors influencing OTA antigen were discussed and the optimum conditions was found by L9(3(4)) orthogonal design. The results showed that when the mole ratio was OTA:BSA = 20:1, OTA:NHS(N-hydroxy-succinamide):DCC(dicyclohexylcarbodiimide) = 1:2:4, the activate time was 120 min and the conjugation time was 90 min, the utilization of OTA could reach 48.2% and the best conjugation ratio of OTA and BSA was 9.64.
Chen
Xuelan
X
Sino-German Joint Research Institute, Nanchang 330047, China.
Xu
Yang
Y
Wu
Chenggang
C
D000946:Antigens, Fungal; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens; D009183:Mycotoxins / Q000032:analysis* / Q000276:immunology; D009793:Ochratoxins / Q000032:analysis* / Q000276:immunology; D013055:Spectrophotometry, Infrared
D000946:Antigens, Fungal; D006241:Haptens; D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A