Identification and quantification of ovine gammaherpesvirus 2 DNA in fresh and stored tissues of pigs with symptoms of porcine malignant catarrhal fever.
Cases of porcine malignant catarrhal fever were analyzed by a combination of identification and quantitation of ovine gammaherpesvirus 2 DNA in a variety of paraffin-embedded tissues from diseased pigs, serology, and exclusion of primary porcine gammaherpesviruses. In spite of reduced signal due to fixation and paraffin embedding, ovine gammaherpesvirus 2 DNA in pig brains exceeded the amounts found in sheep brains by orders of magnitude.
Albini
Sarah
S
Institute of Virology, Veterinary Medical Faculty, University of Zurich, Zurich, Switzerland.
Zimmermann
Werner
W
Neff
Felix
F
Ehlers
Bernhard
B
Häni
Hansjürg
H
Li
Hong
H
Hüssy
Daniela
D
Engels
Monika
M
Ackermann
Mathias
M
D000818:Animals; D001921:Brain / Q000473:pathology / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D018142:Gammaherpesvirinae / Q000302:isolation & purification* / Q000502:physiology; D006566:Herpesviridae Infections / Q000662:veterinary*; D006652:Histological Techniques; D008304:Malignant Catarrh / Q000473:pathology / Q000821:virology*; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology*; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D019562:Viral Load
D004279:DNA, Viral