Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602.
We illustrate the use of a PCR-based method by which the genomic DNA of a microorganism can be rapidly queried for the presence of type I modular polyketide synthase genes to clone and characterize, by sequence analysis and gene disruption, a major portion of the geldanamycin production gene cluster from Streptomyces hygroscopicus var. geldanus NRRL 3602.
Rascher
Andreas
A
Kosan Biosciences, 3832 Bay Center Place, 94545, Hayward, CA, USA. rascher@kosan.com
Hu
Zhihao
Z
Viswanathan
Nina
N
Schirmer
Andreas
A
Reid
Ralph
R
Nierman
William C
WC
Lewis
Matthew
M
Hutchinson
C Richard
CR
D001483:Base Sequence; D016227:Benzoquinones; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000145:classification / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D047029:Lactams, Macrocyclic; D008958:Models, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family; D011809:Quinones / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
D016227:Benzoquinones; D004269:DNA, Bacterial; D047029:Lactams, Macrocyclic; D011809:Quinones; C001277:geldanamycin