title: [Osteocalcin and type I collagen mRNA expression of rat marrow stromal cells under induction condition].
abstract: OBJECTIVE
  To investigate osteocalcin and type I collagen mRNA expression of rat marrow stromal cells under induction condition.
  
  
  METHODS
  Cultured rat marrow stromal cells were induced by osteogenic compound(composed of dexamethasone 10(-3) mol/L, beta glyceralphosphate 10 mmol/L, ascorbic acid 50 micrograms/ml). Osteocalcin and type I collagen mRNA expression were measured using RT-PCR technique.
  
  
  RESULTS
  It was found that rat marrow stromal cells in induced group have an increased time-dependent expression of osteocalcin and type I collagen mRNA, in comparison with control groups. And the necessity of 1,25-(OH)2D3 in induced osteocalcin mRNA expression was confirmed.
  
  
  CONCLUSIONS
  The cultured rat marrow stromal cells kept the undifferentiated state and had osteogenic potential testified by increased osteocalcin and type I collagen mRNA expression when induced.
authors.affiliation: Institute of Biomedical Engineering, West China Medical Center, Sichuan University, Chengdu 610041, China.
authors.forename: Huaiqing
authors.initials: H
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.forename: Jianghua
authors.initials: J
authors.lastname: Lu
authors.forename: Wensheng
authors.initials: W
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D015675:Osteocalcin / Q000096:biosynthesis* / Q000235:genetics; D010012:Osteogenesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017422:Sequence Analysis, DNA; D013250:Steroid Hydroxylases / Q000494:pharmacology; D017154:Stromal Cells / Q000378:metabolism*
chemical_list: D024042:Collagen Type I; D012333:RNA, Messenger; D015675:Osteocalcin; D013250:Steroid Hydroxylases; C406322:vitamin D 1-alpha hydroxylase