[Effects of sterigmatocystin on interleukin-2 secretion of human peripheral blood mononuclear cells in vitro].
Sterigmatocystin (ST) is one of predominant contaminating mycotoxins in foodstuffs and grains of high incidence areas of malignant tumors in China. The effect of ST on interleukin-2 (IL-2) secretion of human peripheral blood mononuclear cells (HPBMc) in vitro was determined with enzyme-linked immunosorbent assay (ELISA) method to explore its putative effects on human immune function. ELISA analysis revealed that ST treatment generally showed negative effects on the IL-2 secretion of HPBMc in vitro. As the ST concentration changes, the inhibiting effects were different. The inhibiting effects at low concentrations (0.03125-0.125 mg/L) and high concentrations (1-2 mg/L) were stronger than the other concentrations(P < 0.05). The time-effect analysis (ST 1 mg/L) showed that inhibiting effects of ST on IL-2 secretion of HPBMc could be seen to a variable degree from 1 to 64 h after ST treatment, while a significant time-effect correlation could be found from 8 to 64 h (r = 0.822, P < 0.05). The results obtained in present study showed that ST has certain inhibiting effects on the secretion of IL-2 of HPBMc in vitro.
Huang
Xianghua
X
Lab. of Experimental Pathology, Hebei Medical University, Shijiazhang 050017, China.
Zhang
Xianghong
X
Yan
Xia
X
Yin
Guiran
G
D002478:Cells, Cultured; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D013241:Sterigmatocystin / Q000633:toxicity*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D007376:Interleukin-2; D013241:Sterigmatocystin