A comparison between disk diffusion and microdilution for susceptibility testing of Mycobacterium fortuitum complex.
We evaluated a disk diffusion method using Mueller-Hinton agar for susceptibility testing of Mycobacterium fortuitum complex organisms. Ninety-five strains were tested both by broth microdilution and disk diffusion. Global results showed good correlation for all antimicrobials except for clarithromycin and erythromycin. However, when the results were analyzed according to species, correlation was poor except for a few antimicrobials. The analysis of the resistant/susceptible results was good for all the antimicrobials tested except azithromycin and erythromycin. In conclusion, the disk diffusion technique could be useful as a screening technique for some antibiotics, but the results must be confirmed by using an accepted reference technique.
Esteban
J
J
Department of Medical Microbiology, Fundación Jiménez Diaz, Madrid, Spain. jesteban@fjd.es
Gadea
I
I
Torres
M V
MV
Cabria
F
F
Rollan
E
E
Santos-O'Connor
F G
FG
Fernández-Roblas
R
R
Soriano
F
F
D000583:Amikacin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017963:Azithromycin / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D017291:Clarithromycin / Q000494:pharmacology; D015169:Colony Count, Microbial / Q000379:methods; D004917:Erythromycin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D019907:Mycobacterium fortuitum / Q000187:drug effects*; D015242:Ofloxacin / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; D004917:Erythromycin; D017963:Azithromycin; D000583:Amikacin; D015242:Ofloxacin; D013752:Tetracycline; D017291:Clarithromycin