title: Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit.
abstract: We have shown that dermatophyte species can be easily identified on the basis of a DNA sequence encoding a part of the large-subunit (LSU) rRNA (28S rRNA) by using the MicroSeq D2 LSU rRNA Fungal Sequencing Kit. Two taxa causing distinct dermatophytoses were clearly distinguished among isolates of the Trichophyton mentagrophytes species complex.
authors.affiliation: Division des Maladies Infectieuses, Hôpital Cantonal Universitaire, 1211 Geneva.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Ninet
authors.forename: Isabelle
authors.initials: I
authors.lastname: Jan
authors.forename: Olympia
authors.initials: O
authors.lastname: Bontems
authors.forename: Barbara
authors.initials: B
authors.lastname: Léchenne
authors.forename: Olivier
authors.initials: O
authors.lastname: Jousson
authors.forename: Renato
authors.initials: R
authors.lastname: Panizzon
authors.forename: Daniel
authors.initials: D
authors.lastname: Lew
authors.forename: Michel
authors.initials: M
authors.lastname: Monod
mesh_terms: D000818:Animals; D003883:Arthrodermataceae / Q000302:isolation & purification*; D001483:Base Sequence; D002415:Cats; D004271:DNA, Fungal / Q000032:analysis; D004285:Dogs; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012339:RNA, Ribosomal, 28S / Q000032:analysis* / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004271:DNA, Fungal; D012339:RNA, Ribosomal, 28S; D011933:Reagent Kits, Diagnostic