title: Rapid detection of adenovirus in throat swab specimens by PCR during respiratory disease outbreaks among military recruits.
abstract: We evaluated the performance of a generic PCR test to detect adenoviruses (AdV) in throat swab specimens collected from asymptomatic and ill military recruits with acute respiratory disease. Samples (n = 210) were collected at entry to basic training and at the time of large outbreaks of AdV-associated acute respiratory disease among military recruits at Fort Jackson, South Carolina, from 1997 to 1998. Compared to cell culture, a sensitivity of 99% and a specificity of 98% were noted for the PCR method to detect AdV in throat swabs. Similar results were obtained with or without DNA extraction, suggesting the absence of significant inhibitors for the PCR method in throat swab samples. No AdV was detected by culture or PCR in throat swabs from healthy recruits, suggesting the absence of latency or asymptomatic shedding. Throat swab specimens proved to be adequate, noninvasive samples to rapidly diagnose respiratory disease in young adults. This generic direct PCR proved to be a useful test for the rapid diagnosis of AdV-associated respiratory disease, detecting all serotypes tested to date and furnishing results within 6 h of specimen arrival. The use of this direct, rapid, sensitive, and specific assay would assist health care providers and public health practitioners in the early diagnosis, management, and control of AdV-associated respiratory disease.
authors.affiliation: Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
authors.forename: Marcela
authors.initials: M
authors.lastname: Echavarria
authors.forename: Jose L
authors.initials: JL
authors.lastname: Sanchez
authors.forename: Shellie A
authors.initials: SA
authors.lastname: Kolavic-Gray
authors.forename: Christina S
authors.initials: CS
authors.lastname: Polyak
authors.forename: Felicia
authors.initials: F
authors.lastname: Mitchell-Raymundo
authors.forename: Bruce L
authors.initials: BL
authors.lastname: Innis
authors.forename: David
authors.initials: D
authors.lastname: Vaughn
authors.forename: Richard
authors.initials: R
authors.lastname: Reynolds
authors.forename: Leonard N
authors.initials: LN
authors.lastname: Binn
mesh_terms: D000256:Adenoviridae / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000453:epidemiology*; D004196:Disease Outbreaks; D006801:Humans; D008889:Military Personnel; D010614:Pharynx / Q000821:virology*; D016133:Polymerase Chain Reaction; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000821:virology*