title: The relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies, Mn-SOD and carotenoids in rural Japanese residents.
abstract: This study investigated the relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies (oLAB), Mn-SOD, and carotenoids. Subjects were 79 males (mean age +/- standard deviation; 62.1 +/- 10.0 years) and 79 females (60.3 +/- 10.3 y) who attended a health examination screening in the town of Hokkaido, Japan. Serum 8-OHdG, Mn-SOD, and oLAB levels were measured by ELISA and serum carotenoids levels were measured by HPLC. Smoking habits were assessed by public health nurses using a questionnaire. Serum 8-OHdG levels were significantly higher in males than in females. On the other hand, serum levels of beta-carotene, alpha-carotene, beta-cryptoxanthin, and zeaxanthin and lutein were significantly lower in males than in females. Serum beta-carotene, beta-cryptoxanthin, and zeaxanthin and lutein were significantly lower in males who were current smokers, compared to non-smokers. Serum 8-OHdG levels were also significantly higher in current smokers. Furthermore, in males, serum oLAB and beta-carotene levels were significantly and negatively correlated with the number of cigarettes smoked per day. Serum Mn-SOD levels were unrelated to smoking habits in males. In conclusion, this preliminary study suggests that cigarette smoking increases serum 8-OHdG levels and reduces serum levels of oLAB and carotenoids, such as beta-carotene, beta-cryptoxanthin, and zeaxanthin and lutein in healthy male subjects. Serum levels of 8-OHdG, oLAB, and carotenoids may be useful biomarkers of oxidative conditions affected by smoking.
authors.affiliation: Department of Public Health, Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Suzuki
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ito
authors.forename: Junichi
authors.initials: J
authors.lastname: Ochiai
authors.forename: Kunio
authors.initials: K
authors.lastname: Aoki
authors.forename: Kenji
authors.initials: K
authors.lastname: Wakai
authors.forename: Akiko
authors.initials: A
authors.lastname: Tamakoshi
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ando
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Kotaro
authors.initials: K
authors.lastname: Ozasa
authors.forename: Nao
authors.initials: N
authors.lastname: Seki
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Nishino
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kondo
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Ohno
authors.forename: Akiko
authors.initials: A
authors.lastname: Tamakoshi
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Mori
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Motohashi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Tsuji
authors.forename: Yosikazu
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Iso
authors.forename: Haruo
authors.initials: H
authors.lastname: Mikami
authors.forename: Shuji
authors.initials: S
authors.lastname: Hashimoto
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Inaba
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Hoshiyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suzuki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Shimizu
authors.forename: Hideaki
authors.initials: H
authors.lastname: Toyoshima
authors.forename: Shinkan
authors.initials: S
authors.lastname: Tokudome
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ito
authors.forename: Shogo
authors.initials: S
authors.lastname: Kikuchi
authors.forename: Akio
authors.initials: A
authors.lastname: Koizumi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kawamura
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Tsuneharu
authors.initials: T
authors.lastname: Miki
authors.forename: Chigusa
authors.initials: C
authors.lastname: Date
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Sakata
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nose
authors.forename: Norihiko
authors.initials: N
authors.lastname: Hayakawa
authors.forename: Takesumi
authors.initials: T
authors.lastname: Yoshimura
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Fukuda
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Okamoto
authors.forename: Hideo
authors.initials: H
authors.lastname: Shio
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Ohno
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kitagawa
authors.forename: Toshio
authors.initials: T
authors.lastname: Kuroki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tajima
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000704:Analysis of Variance; D002338:Carotenoids / Q000097:blood*; D016009:Chi-Square Distribution; D002851:Chromatography, High Pressure Liquid; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D012907:Smoking / Q000097:blood* / Q000453:epidemiology; D013482:Superoxide Dismutase / Q000097:blood*; D011795:Surveys and Questionnaires
chemical_list: D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D002338:Carotenoids; D000080242:8-Hydroxy-2'-Deoxyguanosine; D013482:Superoxide Dismutase; D003849:Deoxyguanosine