Calcyclin (S100A6) regulates pulmonary fibroblast proliferation, morphology, and cytoskeletal organization in vitro.
Calcyclin (S100A6) is a member of the S100A family of calcium binding proteins. While the precise function of calcyclin is unknown, calcyclin expression is associated with cell proliferation and calcyclin is expressed in several types of cancer phenotypes. In the present study, the functional role of calcyclin was further elucidated in pulmonary fibroblasts. Antisense S100A6 RNA expression inhibited serum and mechanical strain-induced fibroblast proliferation. This attenuated proliferative response was accompanied by a flattened, spread cell morphology, and disruption of tropomyosin labeled microfilaments. Changes in cytoskeletal organization did not correspond with a decrease in tropomyosin levels. These observations suggest a role for calcyclin in modulating calcium dependent signaling events that regulate progression through the cell cycle. J. Cell. Biochem. 88: 848-854, 2003.
Breen
Ellen C
EC
Division of Physiology, Department of Medicine, University of California, San Diego, La Jolla, California, USA. ebreen@ucsd.edu
Tang
Kechun
K
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins; D002455:Cell Division; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D005822:Genetic Vectors; D007118:Immunoassay; D066298:In Vitro Techniques; D008168:Lung / Q000196:embryology; D016372:RNA, Antisense / Q000096:biosynthesis; D051381:Rats; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D013314:Stress, Mechanical; D014335:Tropomyosin / Q000032:analysis; D015854:Up-Regulation
D018797:Cell Cycle Proteins; D016372:RNA, Antisense; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C489132:S100a6 protein, rat; D014335:Tropomyosin