[Establishment of mouse ZF-12+/- embryonic stem cells].
By using the mouse zinc finger protein gene ZF-12 genomic DNA fragment, pSSC-TV-10.5 was designed and constructed as a replacement vector. Structure of pSSC-TV-10.5 was identified by restrictive digestion analysis and partly sequencing. Then linearized vector was electroporated into ES cells, and transfected cells were screened by G418 and GANC selection. Among 508 G418r/GANCr colonies, 4 were proved to have taken place the homologous recombination of ZF-12 by PCR and southern blotting analysis. This study lays the foundations of preparing mouse models of ZF-12+/- or ZF-12-/-.
Li
Jian-Zhong
JZ
Department of Medical Genetics, Second Military University, Shanghai 200433, China.
Yang
Hua
H
Fu
Ji-Liang
JL
D000818:Animals; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics*; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018274:Electroporation; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015774:Ganciclovir / Q000494:pharmacology; D005822:Genetic Vectors / Q000235:genetics; D005838:Genotype; D005839:Gentamicins / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014162:Transfection / Q000379:methods; D016335:Zinc Fingers / Q000235:genetics*
D002352:Carrier Proteins; D005839:Gentamicins; C474810:Zfp24 protein, mouse; D004247:DNA; C010680:antibiotic G 418; D015774:Ganciclovir