[The role of ICAM-1 in leukocyte adherence to endothelial cells induced by hypoxia/reoxygenation].
OBJECTIVE
To determine whether intercellular adhesion molecule-1 (ICAM-1) mediats the polymorphonuclear leukocyte (PMN) adherence to endothelial cells elicited by hypoxia/reoxygenation (H/R).


METHODS
Human umbilical vein endothelial cells (HUVEC) monolayers was exposed to hypoxia for 30 min or 120 min then reoxygenated. Count the number of PMN in adhesion assays. Expression of ICAM-1 and ICAM-1 mRNA was detected by immunohistochemical analysis and in situ hybridization.


RESULTS
PMN adhesion to HUVEC exposed to H/R was significantly increased (1 fold) as compared with that of control (P < 0.01). H/R induced hyperadherence was significantly diminished by monoclonal antibodies directed at ICAM-1 or CD11a/CD18. The results showed an increased expression of ICAM-1 and ICAM-1 mRNA on HUVEC exposed to H/R. Hypoxic HUVEC might be responsible for PMN adesion.


CONCLUSIONS
ICAM-1 mediated the adhesive interaction between PMN and VEC induced by hypoxia/reoxygenation.
Qian
G
G
Department of Hemostasis, Thrombosis and Vasculature, Institute of Hematology, CAMS and PUMC, Tianjin 300020.
Liu
H
H
Zhao
Y
Y
D002448:Cell Adhesion; D015687:Cell Hypoxia / Q000502:physiology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology
D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1