[Random amplified polymorphic DNA analysis of Cryptosporidium species and strains].
OBJECTIVE
To analyse the genetic heterogeneity of 8 isolates of Cryptosporidium belonging to three species (Cryptosporiduim muris, Cryptosporiduim parvum and Cryptosporidium meleagridis) using RAPD technique.


METHODS
DNA extracts of the 8 isolates were amplified by PCR using 26 out of 200 primers. The amplified products were separated by agar electrophoresis followed by staining with ethidium bromide.


RESULTS
RAPD analysis revealed; 1. Obvious differences among species were found, the average genetic distance being 7.037; 2. Certain differences among different isolates of the same species were found, the average genetic distance being 5.162.


CONCLUSIONS
RAPD could differentiate the common and respective gene fragments of the 8 isolates of Cryptosporidium.
Tian
Z C
ZC
Department of Preventive Veterinary Science, Quartermaster University of PLA, Changchun 130062.
Zhang
X C
XC
Wang
J M
JM
Yin
J G
JG
Li
J H
JH
Chen
J B
JB
Yang
J
J
D000818:Animals; D002417:Cattle; D002645:Chickens; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics*; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis*; D004372:Ducks; D005243:Feces / Q000469:parasitology; D018740:Genetic Heterogeneity; D051379:Mice; D011817:Rabbits; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D016054:DNA, Protozoan