[A quantitative study of the effect of daunoblastina on argyrophilic nucleolar organiser region-associated proteins in cultured human retinal pigment epithelial cells].
OBJECTIVE
To investigate the effect of daunoblastina on argyrophilic nucleolar organiser region-associated proteins (AgNORs) in cultured human retinal pigment epithelium (RPE) cells.


METHODS
The culture medium of secondary cultured human RPE cells were replaced with a daunoblastina-containing one at a dose of 180 micrograms/L, and the cells were incubated for 12 hr at 37 degrees C. After that, the medium was withdrawn, fresh medium was added and incubation was carried out for an additional 24 hr. AgNORs were studied by using a silver staining technique and image analysis system.


RESULTS
The area of intranuclear AgNORs (micron 2/nucleus) in the control and the daunoblastina-treated group were 41.61 +/- 4.52 and 13.66 +/- 0.65(P < 0.01), and the area density of intranuclear AgNORs(AgNORs/nucleus area ratio)(%) in the two groups were 20.37 +/- 1.97 and 13.06 +/- 2.44(P < 0.01) respectively.


CONCLUSIONS
Inhibiting nucleolar organiser region transcriptional activity may be partly responsible for the cytotoxicity of daunoblastina on the cultured human RPE cells.
Wang
Y
Y
Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xian 710032, China.
Hui
Y
Y
D000328:Adult; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D002478:Cells, Cultured; D003630:Daunorubicin / Q000494:pharmacology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D009687:Nuclear Proteins / Q000032:analysis*; D009697:Nucleolus Organizer Region / Q000737:chemistry* / Q000648:ultrastructure; D010857:Pigment Epithelium of Eye / Q000166:cytology* / Q000648:ultrastructure; D016622:Silver Staining; D013194:Staining and Labeling
D000903:Antibiotics, Antineoplastic; D009687:Nuclear Proteins; D003630:Daunorubicin