[C677T genetic polymorphism of methylenetetrahydrofolate reductase in premature coronary heart disease].
OBJECTIVE
Methylenetetrahydrofolate reductase (MTHFR) is an important factor responsible for hyperhomocysteinemia. The relation of MTHFR gene C677T polymorphism and premature coronary heart disease was studied.


METHODS
MTHFR C677T genetic polymorphisms in 67 patients with premature coronary heart disease were detected by PCR-RFLP technique.


RESULTS
In case group, the frequency of T homogenic type was 34.3% (23/67), heterogenic type 43.3% (29/67) and C homogenic type 22.4% (15/67). T allele frequency was 55.9% (75/134) while C allele frequency 44.1% (59/134) in case group. There were significant differences in MTHFR genotype and allele frequencies between cases and controls (chi 2 = 6.82 and 5.41 respectively, P < 0.05).


CONCLUSIONS
It was suggested that MTHFR gene C677T mutation was a possible risk factor of Chinese premature coronary heart disease.
Xu
H
H
Department of Coronary Heart Diseases, Fuwai Hospital, CAMS and PUMC, Beijing 100037.
Chen
Z
Z
Tang
J
J
Zhu
D
D
Zhang
C
C
D000328:Adult; D000483:Alleles; D003327:Coronary Disease / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D008875:Middle Aged; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D000587:Oxidoreductases Acting on CH-NH Group Donors; D042965:Methylenetetrahydrofolate Reductase (NADPH2)