DNA analysis of hepatocytes: a novel method for estimating the value of liver tissue vaccine (LTV).
Because of the socio-economic condition of African countries, the traditional types of vaccines should be encouraged. LTV in a preliminary study, proved to be of value in reducing morbidity and mortality in Schistosoma mansoni infection. In the present study and because of the vaccine contains liver tissue homogonate, the present authors analyed the DNA content of the hepatocytes of the vaccinated and control groups of animals to know any effects of the vaccine on the hepatocytes ploidy. The DNA content of vaccinated animals was higher. The results were discussed. It was concluded that for testing S. mansoni vaccine, DNA analysis of heptocytes for detecting proliferative S-phase is an important as a positive immune reaction.
El-Bishlawy
I
I
Tropical Medicine Research Institute, Ministry of Health and Population, Cairo 11566, Egypt.
Aly
S M
SM
Fahmy
I A
IA
D000818:Animals; D004247:DNA / Q000032:analysis*; D022781:Hepatocytes / Q000737:chemistry* / Q000166:cytology; D008099:Liver / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D008297:Male; D051379:Mice; D011003:Ploidies; D016196:S Phase; D012550:Schistosoma mansoni / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000517:prevention & control*; D014611:Vaccination; D014612:Vaccines / Q000276:immunology*
D014612:Vaccines; D004247:DNA