[Effect of lidamycin on the expression of genes involved in invasion regulation in HCT-8 human colon cancer cells].
OBJECTIVE
To study the effect of lidamycin on the expression of genes involved in invasion regulation in HCT-8 human colon cancer cells.


METHODS
HCT-8 human colon cancer cells were treated with lidamycin (10 nmol.L-1) for 8 h. The effect of lidamycin on the expression of genes were detected by cDNA arrays. Northern blot and RT-PCR.


RESULTS
Hybridization of the entire cDNA populations to Atlas Arrays showed that lidamycin down-regulated the expression level of MMP-9 and up-regulated the expression level of TIMP-1. These changes were confirmed by Northern blot and RT-PCR.


CONCLUSIONS
The results indicate that lidamycin may exhibit its anti-invasive activity by inhibitting the production of type IV collagenase whilst enhancing the production of tissue inhibitor metalloproteinase.
Cui
D P
DP
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.
Wang
Z
Z
Li
D D
DD
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology; D053281:Enediynes; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000235:genetics; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D053281:Enediynes; D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; C058024:C 1027; D020780:Matrix Metalloproteinase 9