Assisting functional assignment for hypothetical Heamophilus influenzae gene products through structural genomics.
The three-dimensional structures of Haemophilus influenzae proteins whose biological functions are unknown are being determined as part of a structural genomics project to ask whether structural information can assist in assigning the functions of proteins. The structures of the hypothetical proteins are being used to guide further studies and narrow the field of such studies for ultimately determining protein function. An outline of the structural genomics methodological approach is provided along with summaries of a number of completed and in progress crystallographic and NMR structure determinations. With more than twenty-five structures determined at this point and with many more in various stages of completion, the results are encouraging in that some level of functional understanding can be deduced from experimentally solved structures. In addition to aiding in functional assignment, this effort is identifying a number of possible new targets for drug development.
Gilliland
Gary L
GL
Center for Advanced Research in Biotechnology of the University of Maryland Biotechnology Institute and the National Institute of Standards and Technology, 9600 Gudelsky Drive, Rockville, MD 20850, USA. gary.gilliland@nist.gov
Teplyakov
Alexey
A
Obmolova
Galina
G
Tordova
Maria
M
Thanki
Narmada
N
Ladner
Jane
J
Herzberg
Osnat
O
Lim
Kap
K
Zhang
Hong
H
Huang
Kui
K
Li
Zhong
Z
Tempczyk
Aleksandra
A
Krajewski
Wojiech
W
Parsons
Lisa
L
Yeh
Deok Cheon
DC
Orban
John
J
Howard
Andrew J
AJ
Eisenstein
Edward
E
F  Parsons
James
J
Bonander
Nicklas
N
Fisher
Kathryn E
KE
Toedt
John
J
Reddy
Prasad
P
Rao
C V
CV
Melamud
Eugene
E
Moult
John
J
D016679:Genome, Viral; D006193:Haemophilus influenzae / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D011487:Protein Conformation; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology
D014764:Viral Proteins