[Precollagen III in the vitreous of eyes with proliferative vitreoretinopathy].
OBJECTIVE
To detect precollagen III (PC III) levels in serum and vitreous samples from patients with proliferative vitreoretinopathy (PVR).


METHODS
PC III levels were measured by radioimmunoassay in 5 cadaveric vitreous samples from normal subjects, 20 normal human serum samples, 29 vitreous and serum samples from patients with rhegmatogenous retinal detachment complicated with PVR. The relationship between PC III levels and PVR history, surgery, epiretinal membrane, severity of PVR were analyzed with Logistic regression analysis.


RESULTS
The mean PC III levels in serum samples of PVR patients and control subjects were 83.76 +/- 18.52 and 85.02 +/- 17.50 micrograms/L respectively (P > 0.05). PC III was not detected (< 40 micrograms/L) in the cadaveric vitreous. In vitreous aspirates from 29 eyes with PVR, it was not detected in 12 eyes, but higher than 40 micrograms/L in 17 eyes with the mean level of 268.69 +/- 176.07 micrograms/L. (P < 0.05) The high levels correlated with duration and severity of PVR. As onset time and PVR grade increasing, the probability of PC III concentration higher than 40 micrograms/L was 5.2655 and 2.7978 times the concentration lower than 40 micrograms/L respectively.


CONCLUSIONS
PC III can be detected in vitreous aspirates from PVR eyes. The PC III levels in the vitreous was an event of local responses and were correlated with severity and history of PVR. This suggests that PC III and collagen III be involved in the development of PVR.
Feng
X
X
Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xian 710032, China.
Hui
Y
Y
Wang
L
L
D000293:Adolescent; D000328:Adult; D000368:Aged; D024061:Collagen Type III / Q000032:analysis* / Q000097:blood; D005260:Female; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D012163:Retinal Detachment / Q000150:complications / Q000378:metabolism; D018630:Vitreoretinopathy, Proliferative / Q000150:complications / Q000378:metabolism*; D014822:Vitreous Body / Q000378:metabolism*
D024061:Collagen Type III