title: Decrease in fibrin content of venous thrombi in selectin-deficient mice.
abstract: The purpose of this study was to quantify the fibrin content of thrombi produced in a mouse model of venous thrombosis and correlate this to thrombus mass. The role of P-selectin, E-selectin, and IL-10 on thrombus fibrin content was analyzed using knockout (KO) mice. Five groups of mice were evaluated: control (N = 10), P-selectin KO (N = 7), E-selectin KO (N = 5), combined E-/P-selectin KO (N = 12), and IL-10 KO (N = 10). Venous thrombosis was induced by ligation of the infrarenal IVC. Mice were sacrificed on postoperative days (POD) 2 and 6. Thrombus mass was calculated. Sections of IVC were stained with an antibody that cross reacts with mouse fibrin. The distribution of RGB color pixels was generated from digitized micrographs of the thrombus of each animal. The mean pixel value for each group was compiled and analyzed using 2-way ANOVA. Mean pixel value per group was correlated with the mean thrombus mass per group. Color analysis demonstrated significant decreases in the analyzed fibrin content on POD-2 between the control vs E-/P-selectin KO (P < 0.05) and control vs IL-10 KO (P < 0.05) groups. In addition, significantly less fibrin staining was noted on POD-6 between the control vs E-selectin KO (P = 0.03), control vs P-selectin KO (P = 0.01), and control vs E-/P-selectin KO (P < 0.01). There was a strong overall correlation between the mean pixel value for each group and the thrombus mass (R = 0.964; P < 0.01). This study demonstrates a difference in fibrin content of thrombi produced in animals deficient in E-selectin, P-selectin, and IL-10, supporting their importance in thrombus amplification, fibrin formation, and the mass of thrombus formed.
authors.affiliation: Jobst Vascular Research Laboratories, University of Michigan, Department of Surgery, Section of Vascular Surgery, Ann Arbor, Michigan 48109, USA. vitas@umich.edu
authors.forename: V V
authors.initials: VV
authors.lastname: Sullivan
authors.forename: A E
authors.initials: AE
authors.lastname: Hawley
authors.forename: D M
authors.initials: DM
authors.lastname: Farris
authors.forename: B S
authors.initials: BS
authors.lastname: Knipp
authors.forename: A J
authors.initials: AJ
authors.lastname: Varga
authors.forename: S K
authors.initials: SK
authors.lastname: Wrobleski
authors.forename: P
authors.initials: P
authors.lastname: Thanapron
authors.forename: M J
authors.initials: MJ
authors.lastname: Eagleton
authors.forename: D D
authors.initials: DD
authors.lastname: Myers
authors.forename: J B
authors.initials: JB
authors.lastname: Fowlkes
authors.forename: T W
authors.initials: TW
authors.lastname: Wakefield
mesh_terms: D000818:Animals; D019040:E-Selectin / Q000235:genetics / Q000502:physiology*; D005337:Fibrin / Q000032:analysis*; D007150:Immunohistochemistry; D016753:Interleukin-10 / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D007958:Leukocyte Count; D018655:Lymphocyte Count; D051379:Mice; D018345:Mice, Knockout; D008853:Microscopy; D009000:Monocytes; D009504:Neutrophils; D019007:P-Selectin / Q000235:genetics / Q000502:physiology*; D013997:Time Factors; D020246:Venous Thrombosis / Q000378:metabolism* / Q000473:pathology
chemical_list: D019040:E-Selectin; D019007:P-Selectin; D016753:Interleukin-10; D005337:Fibrin