A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation.
Cosmid vectors containing a dominate selectable marker (ble) for complementation cloning of genes in Chlamydomonas reinhardtii were created. The usefulness of these vectors, which differ in the orientation of the ble cassette, was demonstrated by transforming C. reinhardtii to phleomycin resistance, by constructing a large library (approximately 5 x 10(5) recombinants) in one of them using DNA from a C. reinhardtii mutant, and by transforming C. reinhardtii with recombinant cosmid clones and pools.
Chang
Mary
M
Section of Molecular Cell and Developmental Biology (A6700), Institute for Cellular and Molecular Biology, University of Texas at Austin, Bio 311, Austin, TX 78712, USA.
Li
Fei
F
Odom
O W
OW
Lee
Jaesung
J
Herrin
David L
DL
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D016825:Chlamydomonas reinhardtii / Q000187:drug effects / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D003360:Cosmids / Q000235:genetics*; D015723:Gene Library; D005799:Genes, Dominant / Q000235:genetics; D005816:Genetic Complementation Test; D005819:Genetic Markers; D005822:Genetic Vectors; D010692:Phleomycins / Q000494:pharmacology; D012641:Selection, Genetic; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D005819:Genetic Markers; D010692:Phleomycins; C099353:phleomycin resistance protein, Streptoalloteichus hindustanus