[Comparison of three methods in detection of rotavirus infection in neonates].
OBJECTIVE
To compare, three methods in detection of rotavirus infection in neonates.


METHODS
252 fecal samples from neonates with acute gastroenteritis, healthy neonates and under 4-month-old babies with diarrhoea were detected with ELISA, PAGE and EM.


RESULTS
Using ELISA and PAGE to check 5 fecal samples from neonates with diarrhoea, the positive number of ELISA was 4 and the PAGE was all negative. One fecal sample from neonate of one month old with diarrhoea detected with ELISA was strong positive and result with EM was negative. By checking 119 fecal samples from normal neonates of less than ten days old, the positive rate of ELISA was 54.6% and results of PAGE were all RNA negative. 128 fecal samples from less than 4-month-old babies with diarrhoea showed coincident detection rate of ELISA and PAGE (81.2%), but which was going up with increasing of their ages. We also checked 55 fecal samples from less than 4-month-old babies with diarrhoea by ELISA, PAGE, EM and the positive rates were 27.3%, 38.2% and 20% respectively, the coincident rate of the three was 63.6% and the differentiation rate 36.4%.


CONCLUSIONS
The differentiation rate between ELISA and PAGE was obvious, as in the elisa there showed a sirong positive and in PAGE the weak. The diffesence went down with increasing of the ages of the babies with diarrhoea. This indicated that the false positive rate of ELISA existed. PAGE is more reliable and objective and is the better method to check neonate diarrhoea.
Chen
Y
Y
Capital Institute of Pediatrics, Beijing 100020.
Zhao
J
J
Yan
L
L
D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012401:Rotavirus / Q000302:isolation & purification* / Q000648:ultrastructure; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000821:virology*