Experiment study of effect of perfluorohexyloctane on corneal endothelial cells.
OBJECTIVE
To investigate the effect of Perfluorohexyloctane (F6H8) on corneal endothelial cells(CEC) of rabbit eyes.


METHODS
Fifteen New Zealand white rabbits were divided into two groups: experimental group(F6H8) and control group(BSS). All rabbits underwent anterior chamber injection of 0.15 ml F6H8 or BSS. Slit-lamp biomicroscopy and corneal endothelium photography were performed pre-operatively and postoperatively. Histopathological examination and Transmission electron microscopy(TEM) were done after the rabbits were sacrificed.


RESULTS
All the corneas were clear. Since 4 weeks after operation, the endothelial cells were markedly irregular in size and shape and the number of endothelial cells was markedly decreased. Multilayered retrocorneal membranes(RCM) grew gradually 2 weeks after surgery. Vacuolar degeneration was seen in some endothelial cells. Nuclear degeneration and edema of plasma were seen in TEM.


CONCLUSIONS
Corneal endothelial cell degenerated after contacting with F6H8 for 2-4 weeks. As a silicone solvent, it should be removed completely after injection. We don't recommend it to be used as a new intraocular temponade.
Ding
X
X
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Science, Guangzhou 510060, China.
Li
C
C
Lu
L
L
Feng
G
G
Zheng
H
H
D000818:Animals; D000867:Anterior Chamber; D004728:Endothelium, Corneal / Q000473:pathology*; D005260:Female; D005466:Fluorocarbons / Q000494:pharmacology* / Q000633:toxicity; D007267:Injections; D008297:Male; D011817:Rabbits
D005466:Fluorocarbons; C118616:perfluorohexyl-octan