title: [Circulating antigen detection in schistosomiasis japonica patients by sandwich ELISA using polyclonal and monoclonal antibodies].
abstract: OBJECTIVE
  To detect circulating antigen in the patients with schistosomiasis.
  
  
  METHODS
  Polyclonal antibody(PcAb)-monoclonal antibody(McAb) sandwich ELISA.
  
  
  RESULTS
  Sera from 150 patients with schistosomiasis were detected, the average sensitivity was 84.7%(72.0%-91.0%). No false positive reaction was detected in 40 normal controls and none but 2 patients(2/10) with paragonimiasis showed cross reactions among 74 cases with other parasitic infections.
  
  
  CONCLUSIONS
  PcAb-McAb sandwich ELISA is a relatively ideal method for circulatling antigen detection.
authors.affiliation: Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
authors.forename: L S
authors.initials: LS
authors.lastname: Qiu
authors.forename: Z
authors.initials: Z
authors.lastname: Feng
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis / Q000276:immunology*; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth