The minimum LOH region defined on chromosome 17p13.3 in human hepatocellular carcinoma with gene content analysis.
Hepatocellular carcinoma (HCC) is one of the most common human cancers in Asia. Previous studies have shown that in addition to aberrations of the p53 gene on chromosome 17p13.1, other gene(s) on chromosome 17p13.3 may also play a role in HCC. To detect the status of loss of heterozygosity (LOH) in HCC and to determine the minimum region of LOH on 17p13.3, we analyzed 22 paired HCC and non-cancerous liver samples with 14 polymorphic markers plus TP53 (p53 gene) as a comparison. The data revealed a high level of LOH (>68%) in a minimum region between D17S1866 and D17S1574, spanning over a 1.5 Mb region. Genomic library screening using markers in the region has resulted in the isolation of a cluster of BAC/PAC clones. We created a physical map in this region. Using large-scale genome sequencing, gene annotation, cDNA screening, and exon trapping, we identified 17 known genes and 13 novel genes in the minimum region. The function of these genes was analyzed and the possibility of several putative tumor suppressor genes was discussed.
Zhao
Xintai
X
National Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, 2200 Xie Tu Road, 200032, Shanghai, China.
He
Mei
M
Wan
Dafang
D
Ye
Yun
Y
He
Yinghua
Y
Han
Liwei
L
Guo
Minglei
M
Huang
Yi
Y
Qin
Wenxin
W
Wang
Ming Wei
MW
Chong
Wenming
W
Chen
Jianguo
J
Zhang
Lisheng
L
Yang
Nanwu
N
Xu
Binghua
B
Wu
Mengchao
M
Zuo
Lin
L
Gu
Jianren
J
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D020451:Contig Mapping; D005091:Exons / Q000235:genetics; D005796:Genes; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009363:Neoplasm Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA
D009363:Neoplasm Proteins