Yap1 accumulates in the nucleus in response to carbon stress in Saccharomyces cerevisiae.
Yap1 is a transcription factor of the AP-1 family that is required for the adaptive response to oxidative stress in Saccharomyces cerevisiae. We recovered Yap1 in a two-hybrid screen for proteins that interact with the Sip2 subunit of the Snf1 protein kinase, which is required for the adaptation of cells to glucose limitation. Yap1 becomes enriched in the nucleus when cells are subjected to oxidative stress. We show that the localization of Yap1 is similarly sensitive to carbon stress. When glucose-grown cells were shifted to medium containing glycerol or no added carbon source, green fluorescent protein (GFP)-Yap1 accumulated in the nucleus. After adaptation to growth in glycerol, GFP-Yap1 was again primarily cytoplasmic. Nuclear accumulation was independent of respiration and of the Snf1, PKA, TOR, and Yak1 pathways, and the mechanism is distinct from that involved in the response to hydrogen peroxide. Addition of glutathione to the medium inhibited nuclear accumulation of GFP-Yap1 in response to carbon stress but did not affect the relocalization of Gal83 or Mig1. Other stresses such as increased temperature, acidic pH, and ionic stress did not cause nuclear enrichment of GFP-Yap1. These findings suggest a role for Yap1 in the response to carbon stress.
Wiatrowski
Heather A
HA
Department of Genetics and Development, Columbia University, New York, New York 10032, USA.
Carlson
Marian
M
D000222:Adaptation, Physiological / Q000502:physiology; D002244:Carbon / Q000172:deficiency*; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D019069:Cell Respiration / Q000235:genetics; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005947:Glucose / Q000172:deficiency*; D005978:Glutathione / Q000235:genetics / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins; D018384:Oxidative Stress / Q000235:genetics*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics
D002244:Carbon; D017868:Cyclic AMP-Dependent Protein Kinases; D004268:DNA-Binding Proteins; D005947:Glucose; D005978:Glutathione; D005990:Glycerol; D047908:Intracellular Signaling Peptides and Proteins; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor); D017346:Protein Serine-Threonine Kinases; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C085569:GAL83 protein, S cerevisiae; C085976:MIG1 protein, S cerevisiae; C086677:YAP1 protein, S cerevisiae; C071570:SNF1-related protein kinases; C083324:TOR1 protein, S cerevisiae; C062391:YAK1 protein, S cerevisiae