title: The hepatic retinyl ester hydrolase activity is depressed at the onset of diabetes in BB rats.
abstract: Dietary vitamin A as retinyl ester is hydrolysed and re-esterified with long-chain fatty acids in the small intestine. The esterified vitamin A is subsequently stored in the liver, where it is hydrolysed to free retinol to be transported by carrier proteins to the target tissue. A decreased availability of retinol carrier proteins has been suggested to be responsible for affecting metabolic availability of vitamin A in type 1 diabetes. Using BB Wistar rats, the present study was undertaken to examine whether the presence of a hyperglycaemic state modifies retinyl ester hydrolase (REH) activity in the intestine and the liver. At the onset of diabetes, hepatic REH enzymatic activity was significantly decreased. However, REH activity remained unaffected in the small intestine, including both ileum and jejunum. Diabetes also resulted in decreased plasma and liver concentrations of retinol. An in vitro study was conducted to examine the effect of diabetes on the intestinal uptake of retinyl palmitate. Jejunum and ileum from diabetic and non-diabetic BB rats were incubated with labelled retinyl palmitate at different concentrations ranging from 32 to 256 nmol/l. The uptake of retinyl palmitate was increased in both diabetic and non-diabetic rats together with the increase of substrate concentration. However, no significant difference was observed in the uptake of retinyl palmitate between diabetic and non-diabetic rats. These present results suggest that the depressed hepatic REH activities may contribute to the diabetes-associated metabolic derangement of vitamin A.
authors.affiliation: Department of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, Canada.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Alan B R
authors.initials: AB
authors.lastname: Thomson
authors.forename: Andrew T C
authors.initials: AT
authors.lastname: Tsin
authors.forename: Tapan K
authors.initials: TK
authors.lastname: Basu
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002265:Carboxylic Ester Hydrolases / Q000032:analysis* / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology*; D004224:Diterpenes; D007082:Ileum / Q000201:enzymology; D007408:Intestinal Absorption; D007583:Jejunum / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D051381:Rats; D011913:Rats, Inbred BB; D000084562:Retinyl Esters; D014280:Triglycerides / Q000097:blood; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D004224:Diterpenes; D000084562:Retinyl Esters; D014280:Triglycerides; D014801:Vitamin A; C014794:retinol palmitate; D002784:Cholesterol; D002265:Carboxylic Ester Hydrolases; C022523:retinyl esterase