[Assignments of 1HNMR fingerprint of the root bark of Celastrus angulatus].
OBJECTIVE
To assign the 1HNMR finger-print of the root bark of Celastrus angulatus.


METHODS
Silica gel column chromatography was used to separate the chemical constituents of CGE A of the root bark of C. angulatus. The characteristic signals of the 1HNMR finger-print were assigned after determining the structures of the compounds isolated from CGE A.


RESULTS
1HNMR finger-prints of the samples of C. angulatus collected from different regions showed highly characteristic features and reproducibility. Three compounds predominant in CGE A were isolated and their structures were determined by spectral analysis as: angulatin A (1), angulatin B (2) and angulatin C (3).


CONCLUSIONS
Compound 3 is a new compound. Compound 2 was isolated from C. angulatus for the first time. The 1HNMR finger-print of CGE A of the root bark of C. angulatus showed mainly the characteristic signals of the above three compounds and might be utilized for the original authentication of this plant.
Qin
H L
HL
Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China. hlqin@hotmail.com
Zhao
T Z
TZ
Shang
Y J
YJ
Wang
Z T
ZT
D031247:Celastrus / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012717:Sesquiterpenes; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification*
D012717:Sesquiterpenes; D013729:Terpenes; C481915:angulatin C