title: [Establishment of transgenic mouse models carrying human polymeric immunoglobulin receptor gene].
abstract: OBJECTIVE
  To establish transgenic mouse models carrying human polymeric immunoglobulin receptor (pIgR) gene to render the mice prone to EBV infection in normal condition, thereby to facilitate the study of the role of Epstein-Barr virus (EBV) in the pathogenesis of nasopharyngeal carcinoma.
  
  
  METHODS
  By means of microinjection, pIgR gene under the regulation by kerotinocyte-specific promoter ED-L2 was introduced into the pronuclei of mouse zygote, which was transferred into pseudo- pregnant female mice to induce nasopharynx-specific pIgR expression in the founder mice.
  
  
  RESULTS
  Six out of the 16 founder mice (37.5%) was tested by PCR to be pIgR-positive, while Southern blotting identified only 2 positive mice.
  
  
  CONCLUSIONS
  Transgenic mice harboring pIgR gene can be used potentially as a new model for studying the pothogenesis of nasopharyngeal carcinoma by EBV.
authors.affiliation: Institute of Cancer Research, First Military Medical University, Guangzhou 510515, China. lichunlu@fimmu.com
authors.forename: Li-chun
authors.initials: LC
authors.lastname: Lü
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.forename: Ke
authors.initials: K
authors.lastname: Lan
authors.forename: Xin-ming
authors.initials: XM
authors.lastname: Shen
authors.forename: Hua
authors.initials: H
authors.lastname: Huang
authors.forename: Pei-zhou
authors.initials: PZ
authors.lastname: Jiang
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.forename: Shou-jun
authors.initials: SJ
authors.lastname: Xu
authors.forename: Kai-tai
authors.initials: KT
authors.lastname: Yao
mesh_terms: D000818:Animals; D020031:Epstein-Barr Virus Infections; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D019056:Receptors, Polymeric Immunoglobulin / Q000235:genetics* / Q000378:metabolism
chemical_list: D019056:Receptors, Polymeric Immunoglobulin