title: Separation and determination of phenylpropanoid glycosides from Pedicularis species by capillary electrophoresis.
abstract: Capillary zone electrophoresis, using 30 mM borate buffer (pH 9.00) with 10% (v/v) methanol, was established for the identification and determination of four phenylpropanoid glycosides (PPGs)--echinocoside (ECH), verbascoside (VER), pedicularioside M (PED-M) and pedicularioside A (PED-A)--in extracts of Pedicularis longiflora var tubiformis, Pedicularis longiflora and Pedicularis Kansuensis. Regression equations revealed linear relationships (correlation coefficients: 0.9993-0.9999) between the peak area of each compound (ECH, VER, PED-M and PED-A) and its concentration. The relative standard deviations of the migration times and peak areas were <1.93 and 4.54%, respectively. The recoveries of four PPGs ranged between 95.6 and 108.4%. The effects of several CE parameters on the resolutions were studied systematically.
authors.affiliation: Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
authors.forename: Ting-Fu
authors.initials: TF
authors.lastname: Jiang
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: Ou
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Shi
mesh_terms: D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D006027:Glycosides / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D032262:Pedicularis / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D002021:Buffers; D006027:Glycosides