Quantitative liquid chromatography-mass spectrometry determination of catechins in human plasma by automated on-line extraction using turbulent flow chromatography.
A simple, fast and sensitive liquid chromatography-mass spectrometry (LC-MS) method with automated on-line extraction using turbulent flow chromatography (TFC) for the determination of five catechins in human plasma was developed. In this method, after on-line extraction by its injection onto an extractor column at turbulent flow, five catechins were backwashed onto a reversed phase column via on-line column switching and separated chromatographically at a laminar flow of 1 ml min(-1). Using this tandem LC-LC-MS system, the extraction, the separation and the quantitation of five catechins in human plasma could be achieved with satisfactory selectivity and sensitivity. The limit of detection (S/N = 3) ranged from 0.6 to 2 ng ml(-1). The described procedure was very simple and rapid since no off-line sample preparation was required, total analysis time being 18.5 min.
Takino
Masahiko
M
Yokogawa Analytical Systems Inc., 3-3-11, Niitaka, Yodogawa-ku, Osaka 532-0033, Japan. masahiko_takino@agilent.com
Daishima
Shigeki
S
Yamaguchi
Kenji
K
Nakahara
Taketoshi
T
D002392:Catechin / Q000097:blood*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity
D002392:Catechin