Evaluation of vinylsulfamides as sulfhydryl selective alkylation reagents in protein modification.
The preparation of several model vinylsulfamides is described. Their excellent selective reactivity towards sulfhydryl group with regards to amino group has been demonstrated by the kinetics study between a model vinylsulfamide and cysteine or lysine at different pHs.
Li
Min
M
Roche Diagnostics Corporation, 9115 Hague Road, Indianapolis, IN 46250, USA. minli88@att.net
Wu
Robert S
RS
Tsai
Jane S C
JS
Salamone
Salvatore J
SJ
D000477:Alkylating Agents / Q000737:chemistry*; D005030:Ethylenes / Q000737:chemistry*; D006207:Half-Life; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007700:Kinetics; D011506:Proteins / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013439:Sulfhydryl Reagents / Q000737:chemistry*; D013449:Sulfonamides / Q000737:chemistry*
D000477:Alkylating Agents; D005030:Ethylenes; D007202:Indicators and Reagents; D011506:Proteins; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; D013449:Sulfonamides