title: Characterization of cry1, cry2, and cry9 genes in Bacillus thuringiensis isolates from China.
abstract: Bacillus thuringiensis isolates from different ecological regions and sources of China were analyzed to study the distribution and diversity of cry genes and to detect the presence of novel cry genes. Strains containing cry1-type genes were the most abundant and represent 237 of the 310 B. thuringiensis isolates (76.5%). About 70 and 15.5% of the isolates contained a cry2 gene or cry9 gene, respectively, while 10.0% of the strains did not contain a cry1, cry2, or cry9 gene. Among the cry1 containing isolates, cry1A (67.7%), cry1I (60.6%), cry1C (43.9%), and cry1D (39.4%) genes were the most abundant. Forty-three different cry1 gene profiles were detected in this collection. Several cry1 genes were associated at a high frequency, such as the cry1C-cry1D and cry1A-cry1I gene combination. The cry1A and cry2 amplicons were digested with selected restriction enzymes to examine sequence diversity. Based on this RFLP analysis, one novel cry1A-type gene was observed.
authors.affiliation: Department of Microbiology, College of Life Sciences, Nankai University, 94 Weijin Road, 300071, Tianjin, PR China. tracywang70@hotmail.com
authors.forename: Jinhong
authors.initials: J
authors.lastname: Wang
authors.forename: Annemie
authors.initials: A
authors.lastname: Boets
authors.forename: Jeroen
authors.initials: J
authors.lastname: Van Rie
authors.forename: Gaixin
authors.initials: G
authors.lastname: Ren
mesh_terms: D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D002681:China; D056931:Cryptochromes; D029721:Drosophila Proteins; D004731:Endotoxins / Q000235:genetics*; D005136:Eye Proteins; D005420:Flavoproteins / Q000235:genetics*; D020869:Gene Expression Profiling; D006460:Hemolysin Proteins; D017956:Photoreceptor Cells, Invertebrate; D043562:Receptors, G-Protein-Coupled
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D056931:Cryptochromes; D029721:Drosophila Proteins; D004731:Endotoxins; D005136:Eye Proteins; D005420:Flavoproteins; D006460:Hemolysin Proteins; D043562:Receptors, G-Protein-Coupled; C119828:cry protein, Drosophila; C030956:insecticidal crystal protein, Bacillus Thuringiensis