title: [Cloning and sequence analysis of a gene encoding amastin from Leishmania major].
abstract: OBJECTIVE
  To clone a gene encoding surface protein from Leishmania major.
  
  
  METHODS
  Using T. cruzi amastin DNA sequence as a reference, computer search was done on GenBank and dbEST databases by using BLAST path. A Leishmania major DNA library has been constructed and screened by in situ colony hybridization.
  
  
  RESULTS
  A 309nt DNA fragment from Leishmania major was found in dbEST. Leishmania major DNA library was screened using specific primers synthesized according to 309 nt DNA sequence, and a full-length coding sequence for Leishmania major amastin was cloned. The coding sequence consisted of 552 nt, and translated into 183 amino acid residues. The homology is 23.5% at amino acid sequence level between Leishmania major and T. cruzi amastins.
  
  
  CONCLUSIONS
  A full length amastin coding gene for Leishmania major has been cloned.
authors.affiliation: Gene Therapy Research Center, Institute of Infectious Diseases, 302 Hospital of PLA, Beijing 100039.
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: C W
authors.initials: CW
authors.lastname: Si
authors.forename: Q H
authors.initials: QH
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: Y W
authors.initials: YW
authors.lastname: Zhong
authors.forename: J Z
authors.initials: JZ
authors.lastname: Yang
authors.forename: W G
authors.initials: WG
authors.lastname: Hong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics*; D015723:Gene Library; D018320:Leishmania major / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D016054:DNA, Protozoan