[Distribution of human hsp90 beta gene in active chromatin upon heat shock].
OBJECTIVE
To study the distribution of human hsp90 beta gene in active chromatin of Jurkat cells subjected to heat shock.


METHODS
Active and inactive chromatin were isolated by using organomercurial affinity chromatography from Jurkat cells with or without heat shock. Slot-blot hybridization was then carried out with human hsp90 beta cDNA probe. And the efficiency of mRNA expression was studied by Northern blot hybridization.


RESULTS
The content of the hsp90 beta gene distributed in the active chromatin was increased upon heat shock which was comparable with the highly efficient mRNA expression induced by the same treatment.


CONCLUSIONS
The results suggested chromatin activity was a prerequisite for heat shock induction of hsp90 beta gene.
Shi
Y
Y
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing, 100005.
Shen
Y
Y
D002843:Chromatin / Q000378:metabolism*; D015870:Gene Expression; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D006358:Hot Temperature; D006801:Humans; D019169:Jurkat Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics
D002843:Chromatin; D018841:HSP90 Heat-Shock Proteins; D012333:RNA, Messenger