DNA profiling of azoospermic semen samples from vasectomized males by using Y-PLEX 6 amplification kit.
Post-vasectomized azoospermic semen samples (N = 6) were analyzed for short tandem repeats (STR) on the Y-chromosome by using Y-PLEX 6 and the 310 Genetic Analyzer. We have observed a wide variation in the yield of extracted DNA from 12.5-1,000 ng. This variation was attributed to the number of epithelial and/or white blood cells that are present in these azoospermic samples. DNA profiles of these vasectomized males were obtained for all six Y-STR loci, namely DYS393, DYS 19, DYS389II, DYS390, DYS391, and DYS385 amplified by using the Y-PLEX 6.
Shewale
Jaiprakash G
JG
ReliaGene Technologies, Inc., 5525 Mounes St., Suite 101, New Orleans, LA 70123, USA.
Sikka
Suresh C
SC
Schneida
Elaine
E
Sinha
Sudhir K
SK
D000483:Alleles; D041322:Chromosomes, Human, Y; D004247:DNA / Q000032:analysis*; D005838:Genotype; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012661:Semen / Q000737:chemistry; D020080:Tandem Repeat Sequences; D014659:Vasectomy
D011933:Reagent Kits, Diagnostic; D004247:DNA