Characterization of a new integron containing VIM-2, a metallo- beta-lactamase gene cassette, in a clinical isolate of Enterobacter cloacae.
We report the first description of a new integron containing bla(VIM-2), a metallo-beta-lactamase gene from Enterobacter cloacae KU680, which was isolated from peritoneal fluid of a liver cirrhosis patient in South Korea. Antibiotic susceptibility testing, and modified Hodge and EDTA-disc synergy tests, were carried out to screen for metallo-beta-lactamase-producing strains. PCR and sequence analysis were used to identify and analyse the bla(VIM-2)-containing integron. The isolate was resistant to most beta-lactams, including imipenem, and demonstrated a positive modified Hodge and EDTA-disc synergy test, which are findings suggesting a metallo-beta-lactamase. Preliminary PCR-based experiments detected the metallo-beta-lactamase gene bla(VIM-2). Sequencing of the 4392 bp cloned PCR amplicon, containing the gene cassette bla(VIM-2), revealed the structure of the class 1 integron. The integron also contained additional insert gene cassettes, aadA, and unknown open reading frames 'orfII' and 'orfIII'. To the best of our knowledge, this is the first time that this metallo-beta-lactamase gene has been detected in E. cloacae.
Jeong
Seok Hoon
SH
Departments of Clinical Pathology, Internal Medicine and Therapeutic Radiology, Kosin University College of Medicine, Amnam-Dong 34, Seo-Ku, Busan, South Korea. kscpjsh@hs.kosinmed.or.kr
Lee
Kyungwon
K
Chong
Yunsop
Y
Yum
Jong Hwa
JH
Lee
Sang Hee
SH
Choi
Hyuk Jin
HJ
Kim
Jeong Man
JM
Park
Kil Ho
KH
Han
Byung Hoon
BH
Lee
Sang Wook
SW
Jeong
Tae Sik
TS
D000595:Amino Acid Sequence; D001483:Base Sequence; D016972:Enterobacter cloacae / Q000201:enzymology / Q000235:genetics*; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D032023:Integrons / Q000235:genetics*; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics*
C425108:beta-lactamase bla(vim-2); D001618:beta-Lactamases