title: [Sequencing of cytochrome C oxidase 1 gene of Ancylostoma duodenale and Necator americanus].
abstract: OBJECTIVE
  To identify the genetic diversity between Ancylostoma duodenale and Necator americanus.
  
  
  METHODS
  Mitochondrial cytochrome C oxidase subunit 1 (CO1) gene was amplified from genomic DNA of human hookworms collected from infected patients in Hejiang County, Sichuan Province, and the purified PCR products were directly sequenced by using Licor auto-sequencer.
  
  
  RESULTS
  The PCR products were about 700 bp. Alignment of CO1 gene fragment sequences showed 89.7% similarity between Ancylostoma duodenale and Necator americanus, but still certain nucleotide variations (10.3%) existed.
  
  
  CONCLUSIONS
  CO1 gene sequence can be used as a marker to identify the two species of human hookworms.
authors.affiliation: Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
authors.forename: T
authors.initials: T
authors.lastname: Li
authors.forename: B
authors.initials: B
authors.lastname: Zhan
authors.forename: J M
authors.initials: JM
authors.lastname: Hawdon
authors.forename: X
authors.initials: X
authors.lastname: Gong
authors.forename: S
authors.initials: S
authors.lastname: Xiao
authors.forename: Q
authors.initials: Q
authors.lastname: Shan
authors.forename: Z
authors.initials: Z
authors.lastname: Feng
authors.forename: P J
authors.initials: PJ
authors.lastname: Hotez
mesh_terms: D000595:Amino Acid Sequence; D000722:Ancylostoma / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D003578:Cytochrome-c Peroxidase / Q000737:chemistry / Q000235:genetics*; D018509:DNA, Helminth / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D017221:Necator americanus / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D018509:DNA, Helminth; D003578:Cytochrome-c Peroxidase