Immunohistochemical analysis for histopathological subtypes in pediatric medulloblastomas.
Medulloblastomas occurring in children represent a histological spectrum of varying anaplasia and nodularity. In order to determine whether immunohistochemical markers might be useful parameters in subclassifying these tumors, 17 pediatric medulloblastomas, including nine diffuse/non-anaplastic, four diffuse/anaplastic, three nodular/non-anaplastic and one nodular/anaplastic subtypes, were studied. In the present report, we investigate the expression of neural cell adhesion molecule (NCAM), nerve growth factor receptor (NGFR), neurofilament (NF), synaptophysin (SYN), glial fibrillary acidic protein (GFAP), S100, Bcl-2, and Ki-67 by using the immunohistochemistry against specific antibodies. This study showed that NGFR, NF, GFAP and S100 were not detected in anaplastic subtypes of medulloblastomas (0/5), while non-anaplastic subtypes were mainly expressed within the nodules. All 17 tumors were reactive for NCAM, SYN and Bcl-2. In addition, Ki-67 labeling indices for anaplastic subtypes (39.0 +/- 7.42%) were significantly higher than that of non-anaplastic medulloblastomas (11.4 +/- 8.04%; P < 0.0001). These results suggest that immunohistochemical markers are a useful adjunct in characterizing subtypes of pediatric medulloblastomas.
Son
Eun-Ik
EI
Department of Neurosurgery, Institute for Medical Science, Keimyung University School of Medicine, Deagu, Korea.
Kim
Il-Man
IM
Kim
Dong-Won
DW
Yim
Man Bin
MB
Kang
Yu-Na
YN
Lee
Sang-Sook
SS
Kwon
Kun-Young
KY
Suh
Seong-Il
SI
Kwon
Taeg-Kyu
TK
Lee
Jung-Jeung
JJ
Kim
Dong-Sug
DS
Kim
Sang-Pyo
SP
D000293:Adolescent; D014408:Biomarkers, Tumor / Q000378:metabolism; D002528:Cerebellar Neoplasms / Q000145:classification* / Q000378:metabolism / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008527:Medulloblastoma / Q000145:classification* / Q000378:metabolism / Q000473:pathology*; D009363:Neoplasm Proteins / Q000378:metabolism
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins