[DNA cloning and partial sequence analysis of EBV LMP1 gene isolated from human nasopharyngeal carcinoma cell line SUNE1].
OBJECTIVE
To study the variation of EBV LMP1 encoding gene isolated from human nasopharyngeal carcinoma cell line SUNE1 in Guangdong.


METHODS
The EBV LMP1 gene was amplified from the SUNE1 cell genome by PCR and then the recombinant vector was constructed by inserting the PCR fragment into pcDNA3. The encoding EBV LMP1 spanning from exon1 to exon3 in recombinant vector was sequenced by the Sequence Analyzer.


RESULTS
The 1,312 bp encoding EBV LMP1 pieces in 2.6 kb PCR fragments were compared with the same EBV segments in B95-8 cell line. The data indicated that the rate of nucleotide sequence homology between the two fragments is 98.5% and the rate of amino acid is 96%. The restricted enzyme site of Xho I in exon1 was deleted in SUNE1 but the 30 bp deletion at the carboxyl terminus in most Chinese NPC LMP1 gene was not present.


CONCLUSIONS
Although the LMP1 gene derived from SUNE1 had greater tumorigenicity than that derived from B95-8 cell, the high homology rate of nucleotide and amino acid sequences between them indicated that it was not the result from the variation of certain nucleotide sites but the change in amino acid domain.
Chen
Y
Y
Department of Microbiology, Sun Yat-sen University of Medical Sciences, Guangzhou, 510089.
Guo
H
H
Cao
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D020031:Epstein-Barr Virus Infections / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000821:virology*; D014407:Tumor Cells, Cultured; D014763:Viral Matrix Proteins / Q000235:genetics*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins