title: [In vitro uranyle affinity of per (3,6-anhydro-2-O-carboxyméthyle)-alpha-cyclodextrin and conditions required for in vivo application].
abstract: Per (3.6-anhydro-2-O-carboxymethyle)- alpha-cyclodextrin ([1]) is a polydentate analog of EDTA, a well-known cation chelating reagent. [1] exhibits strong affinities in vitro for lanthanids, cobalt and also for uranyl cations. Hence, a 1:1 stoechiometry and a high affinity for uranyle (6<logK<7) is found in vitro. Moreover, [1] is not hemolytic and exhibits not lettral properties in mice (LD(50)=42mM). In vivo injection at supralethal amounts of uranyl complex of [1] prevents immediate death in mice while unable to protect against later death. Pharmacocinetic studies show that a dissociation of the complex occurs lead to the release of free uranyle. Other complexation assays using [1] grafted tissues show that chelating properties for lead and uranyle differ from thoses observed in vitro.
authors.affiliation: CRSSA/BCM, La Tronche.
authors.forename: J C
authors.initials: JC
authors.lastname: Debouzy
authors.forename: A
authors.initials: A
authors.lastname: Gadelle
authors.forename: H
authors.initials: H
authors.lastname: Tymen
authors.forename: B
authors.initials: B
authors.lastname: Le Gall
authors.forename: X
authors.initials: X
authors.lastname: Millot
authors.forename: P
authors.initials: P
authors.lastname: Moretto
authors.forename: F
authors.initials: F
authors.lastname: Fauvelle
authors.forename: M
authors.initials: M
authors.lastname: Le Peoc'H
authors.forename: V
authors.initials: V
authors.lastname: Dabouis
authors.forename: B
authors.initials: B
authors.lastname: Martel
mesh_terms: D000818:Animals; D002614:Chelating Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D003505:Cyclodextrins / Q000737:chemistry*; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D017974:Uranium Compounds / Q000037:antagonists & inhibitors* / Q000493:pharmacokinetics / Q000633:toxicity*; D047391:alpha-Cyclodextrins
chemical_list: C472889:(3,6-anhydro-2-O-carboxymethyle)-cyclodextrin; D002614:Chelating Agents; D003505:Cyclodextrins; D017974:Uranium Compounds; D047391:alpha-Cyclodextrins