Effects of lysogeny of Shiga toxin 2-encoding bacteriophages on pulsed-field gel electrophoresis fragment pattern of Escherichia coli K-12.
Escherichia coli K-12 lysogens of three different Shiga toxin 2 (Stx2)-encoding bacteriophages were examined for variability in their pulsed-field gel electrophoresis (PFGE) fragment patterns. The PFGE fragment patterns could be classified into three types (i.e., PFGE types B, C, and D). For the PFGE type D, a 255-kbp fragment present in the original K-12 strain was apparently shifted by the size of Stx 2-encoding phage genomic DNA (ca. 65 kbp) to the position at 320 kbp. In contrast, the types B and C showed the above fragment shift plus further 6- and 10-fragment differences, respectively, from the original K-12 strain. The evidence suggests that even a single genetic event like lysogeny can cause marked genotypic modification of the host strain.
Iguchi
Atsushi
A
Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe 657-8501, Japan.
Osawa
Ro
R
Kawano
Junichi
J
Shimizu
Akira
A
Terajima
Jun
J
Watanabe
Haruo
H
D001435:Bacteriophages / Q000235:genetics* / Q000254:growth & development; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000821:virology*; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification; D008242:Lysogeny; D022641:Shiga Toxin 2 / Q000235:genetics*
D004279:DNA, Viral; D022641:Shiga Toxin 2