title: [Studies on immunological reaction of the antiserum of recombinant secreted protein from Ancylostoma caninum].
abstract: OBJECTIVE
  To analyze the immunological reaction of the antiserum of recombinant secreted protein from Ancylostoma caninum with antigens of various species hookworms at different developmental stages.
  
  
  METHODS
  SDS-PAGE and ELIB technique were employed in the study.
  
  
  CONCLUSIONS
  The protein component of Ac-rAsp-1 was 45 kDa, its immune serum can recognize the antigens of Ac-L3 and Ac-rAsp-1 protein, but not react to the antigens of Ad-A, Ad-L3, Na-A, Ac-A, Nb-A and Ac-rAsp-2 protein. The protein component of Ac-rAsp-2 was 24 kDa, its immune serum can recognize the antigens of Ad-A, Ad-L3, Na-A, Ac-A, Ac-L3 and Ac-rAsp-2 protein, but not react to the antigens of Nb-A and Ac-rAsp-1 protein.
authors.affiliation: Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou 310013.
authors.forename: L Y
authors.initials: LY
authors.lastname: Wen
authors.forename: P J
authors.initials: PJ
authors.lastname: Hotez
mesh_terms: D000722:Ancylostoma / Q000276:immunology*; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D004285:Dogs; D015801:Helminth Proteins / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: C099548:ASP protein, Ancylostoma caninum; D000947:Antigens, Helminth; D015801:Helminth Proteins; D007106:Immune Sera; D011994:Recombinant Proteins