[Studies on the features of protective immune response induced by recombinant Sjc26GST of Schistosoma japonicum].
OBJECTIVE
To compare the differences in the protective immune response induced by reSjc26GST between C57BL/6 and BALB/c mice.


METHODS
Mice were immunized subcutaneously with reSjc26GST emulsified with Freund's adjuvant. The specific antibody isotypes elicited by reSjc26GST, in vitro lymphocyte proliferation and cytokine responses to reSjc26GST were examined.


RESULTS
The level of anti-reSjc26GST IgG was higher in immunized C57BL/6 mice than in BALB/c mice, and examination of the IgG subclasses demonstrated that reSjc26GST-immunized C57BL/6 mice resulted in predominantly IgG1 and IgG2a/IgG2b antibody responses, whereas in BALB/c mice, IgG1 antibody. Cytokine production assays revealed that the quantity of IFN-gamma and IL-2 and IL-5 released from the reSjc26GST-primed splenocytes were significantly higher in C57BL/6 mice than in BALB/c mice. These results showed that the immunization with reSjc26GST in C57BL/6 mice elicited a mixed immune reaction of Th1 and Th2 cell responses as demonstrated by the elevated levels of IgG1, IgG2a/IgG2b and IL-2, IFN-gamma, IL-5, whereas in BALB/c mice, reSjc26GST immunization elicited Th2 response as demonstrated by the elevated levels of IgG1, IL-2 and IFN-gamma.


CONCLUSIONS
Immunization with reSjc26GST could elicit higher humoral and cellular immune responses in C57BL/6 mice than in BALB/c mice.
Zhou
S
S
Department of Immunology, Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Liu
S
S
Song
G
G
Xu
Y
Y
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D005260:Female; D005982:Glutathione Transferase / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000276:immunology; D012549:Schistosoma japonicum / Q000201:enzymology / Q000276:immunology*
D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D005982:Glutathione Transferase