title: [Establishment of A + G ligation mediated polymerase chain reaction and its application in in vivo footprinting study].
abstract: OBJECTIVE
  To establish a new ligation mediated PCR method for the work in in vivo footprinting study of some regions of low G-residue content or some regions of G-residue free.
  
  
  METHODS
  The A > G chemical cleavage method of Maxam-Gilbert sequencing was modified for ligation mediated PCR after A + G chemical cleavage and separate the PCR products by sequencing PAGE.
  
  
  RESULTS
  A new A + G ligation mediated method was established and could be used in vivo footprinting study.
  
  
  CONCLUSIONS
  Our results indicated that the newly developed method could analyze the DNA-Protein interaction at both A-residue and G-residue, enabled us to get more information and enlarged the scope of its application.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, PUMC and CAMS, Beijing 100005.
authors.forename: X
authors.initials: X
authors.lastname: Ji
authors.forename: D
authors.initials: D
authors.lastname: Liu
authors.forename: D
authors.initials: D
authors.lastname: Xu
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology; D007942:Leukemia, Experimental / Q000473:pathology; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D023302:Protein Footprinting / Q000379:methods*; D014407:Tumor Cells, Cultured