title: Effects of salivary gland extract from Rhipicephalus sanguineus on IgG subclass production and cytokine mRNA expression in mononuclear cells of canine peripheral blood.
abstract: The effects of salivary gland extract (SGE) from R. sanguineus were examined on the production of IgG1 and IgG2 and the mRNA expression of IFN-gamma, IL-2, IL-4, IL-5 and IL-10 in the mononuclear cells from canine peripheral blood, treated with concanavalin A (ConA) in vitro. SGE suppressed the ConA-induced production of IgG2. It also inhibited the expression of IFN-gamma, IL-2 and IL-5 mRNA in a dose-dependent manner. No dose-dependent suppression was observed of IL-10 mRNA expression although a significant effect was observed at a SGE protein concentration of 25 microg/ml. SGE had no effect on the mRNA expression of IL-4. These results suggest that the suppression of IgG2 production by SGE from R. sanguineus was caused by the suppression of IFN-gamma production.
authors.affiliation: Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, Japan.
authors.forename: Koutaro
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Hisashi
authors.initials: H
authors.lastname: Inokuma
authors.forename: Masaru
authors.initials: M
authors.lastname: Okuda
authors.forename: Takafumi
authors.initials: T
authors.lastname: Onishi
mesh_terms: D000818:Animals; D002457:Cell Extracts / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics*; D004285:Dogs; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005786:Gene Expression Regulation; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000235:genetics; D007378:Interleukins / Q000235:genetics; D026863:Ixodidae / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012469:Salivary Glands / Q000276:immunology*
chemical_list: D002457:Cell Extracts; D016207:Cytokines; D007074:Immunoglobulin G; D007378:Interleukins; D012333:RNA, Messenger; D007371:Interferon-gamma