title: [Comparative effect of different media in in vitro cultivation of Trichomonas vaginalis].
abstract: OBJECTIVE
  To investigate the optimal condition for in vitro cultivation of Trichomonas vaginalis for obtaining a better harvest of T. vaginalis.
  
  
  METHODS
  An isolate of T. vaginalis from clinical specimens was cultivated in three different media with initial inoculation of 9.0 x 10(4)/ml under pH 5.6.
  
  
  RESULTS
  There was distinct difference after 96 h incubation in the cumulative harvest of T. vaginalis. The highest harvest was received in cysteine/liver/peptone/maltose medium, followed by the liver/peptone/maltose medium and soybean/liver/peptone/maltose medium.
  
  
  CONCLUSIONS
  The cysteine/liver/peptone/maltose medium may be a suitable environment for in vitro multiplication of T. vaginalis.
authors.affiliation: Department of the Third Centre, Norman Bethune University of Medical Sciences, Changchun 130021.
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Geng
authors.forename: Shu-mei
authors.initials: SM
authors.lastname: Han
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Yu-lu
authors.initials: YL
authors.lastname: Shi
authors.forename: Shu-hong
authors.initials: SH
authors.lastname: Li
mesh_terms: D000818:Animals; D003470:Culture Media; D014246:Trichomonas vaginalis / Q000254:growth & development*
chemical_list: D003470:Culture Media