Role of buffer concentration and applied voltage in obtaining a good separation in planar electrochromatography.
Planar electrochromatography is performed by applying an electric field across a thin layer chromatography (TLC) plate. In addition to electroosmotic flow in the axial direction, there is also flow to the surface of the TLC layer, and this can substantially degrade the quality of separation. This effect is offset by Joule heating which causes evaporation of liquid from the layer surface, and which under some conditions causes degradation of separation quality by excessive drying of the layer. It is shown that pH, buffer concentration, and applied voltage control the balance between liquid being driven to the surface and liquid evaporating from the surface due to Joule heating. Conditions are discussed which result in good separation quality, or in separations degraded by either excessive wetting or drying of the layer. The above separations were performed at constant voltage. A chromatogram is presented that shows that a good separation is also obtained at constant power, i.e. under conditions where there is a constant amount of Joule heating.
Nurok
David
D
Department of Chemistry. Indiana University Purdue University Indianapolis, 402 N. Blackford Street, Indianapolis, IN 46202, USA. nurok@chem.iupui.edu
Koers
James M
JM
Carmichael
Matthew A
MA
D002021:Buffers; D002855:Chromatography, Thin Layer / Q000379:methods*
D002021:Buffers