Dibenzyl sulfide metabolism by white rot fungi.
Microbial metabolism of organosulfur compounds is of interest in the petroleum industry for in-field viscosity reduction and desulfurization. Here, dibenzyl sulfide (DBS) metabolism in white rot fungi was studied. Trametes trogii UAMH 8156, Trametes hirsuta UAMH 8165, Phanerochaete chrysosporium ATCC 24725, Trametes versicolor IFO 30340 (formerly Coriolus sp.), and Tyromyces palustris IFO 30339 all oxidized DBS to dibenzyl sulfoxide prior to oxidation to dibenzyl sulfone. The cytochrome P-450 inhibitor 1-aminobenzotriazole eliminated dibenzyl sulfoxide oxidation. Laccase activity (0.15 U/ml) was detected in the Trametes cultures, and concentrated culture supernatant and pure laccase catalyzed DBS oxidation to dibenzyl sulfoxide more efficiently in the presence of 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) than in its absence. These data suggest that the first oxidation step is catalyzed by extracellular enzymes but that subsequent metabolism is cytochrome P-450 mediated.
Van Hamme
Jonathan D
JD
National Centre for Upgrading Technology, Devon, Alberta T9G 1A8, Canada. jvanhamme@alumni.uwaterloo.ca
Wong
Eddie T
ET
Dettman
Heather
H
Gray
Murray R
MR
Pickard
Michael A
MA
D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development; D001593:Benzyl Compounds / Q000378:metabolism*; D003470:Culture Media; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D042845:Laccase; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D020075:Phanerochaete / Q000378:metabolism; D013997:Time Factors
D001593:Benzyl Compounds; D003470:Culture Media; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D042845:Laccase; C016367:benzyl sulfide