title: An experimental study of the tumour necrosis factor level in aqueous humor after transscleral fixation of intraocular lens.
abstract: OBJECTIVE
  The tumour necrosis factor (TNF) level in aqueous humor after transscleral fixation of intraocular lens (IOL) implantation in rabbits and discuss the effect of TNF on postoperative anterior ocular inflammation.
  
  
  METHODS
  Twenty-seven pigmented rabbits were divided into three groups. Group 1: transscleral fixation of posterior chamber (PC) IOL implantation; Group 2: Lens of rabbits was removed without IOL implantation; Group 3: the control group, without surgical intervention. On the 1st, 3rd, 7th and 14th postoperative days, aqueous humor samples were obtained. An modified double antibodies indirect sandwich ELISA was used to detected for the presence of TNF. The data were analyzed by using analysis of variance of SAS software.
  
  
  RESULTS
  It was found that TNF level in aqueous humor was increased after transscleral fixation of IOL implantation. TNF level reached its maximum on the 14th postoperative day in the IOL implanted group. TNF level on 1st, 3rd, 7th and 14th days postoperatively was significantly the highest (p < 0.05) in IOL implanted group when compared with the other two groups.
  
  
  CONCLUSIONS
  There is something physically related to the IOL itself which induces the secretion of TNF or this simply related to mechanical factors related to the presence of the IOL itself, and the manipulation of the iris during lens implantation stimulates TNF secretion. The increase of TNF level may play critical role in anterior ocular inflammation response after IOL implantation.
authors.affiliation: Department of Ophthalmology, No. 304 Hospital, Beijing 100037, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhou
authors.forename: S
authors.initials: S
authors.lastname: He
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D018916:Blood-Aqueous Barrier; D007249:Inflammation / Q000378:metabolism; D019654:Lens Implantation, Intraocular / Q000009:adverse effects*; D011184:Postoperative Period; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha