title: Extramedullary blast crisis of chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation mimicking aggressive, translocation t(14;18)-positive B-cell lymphoma.
abstract: We report the case of a 42-year-old male patient who was diagnosed with a large tumor of the right thoracic aperture 30 months after unrelated hematopoietic stem cell transplantation (HSCT) for accelerated phase of Philadelphia chromosome (Ph)-positive chronic myeloid leukemia (CML). Biopsy revealed an immature lymphoid neoplasia with blastic tumor cell morphology and immunoreactivity for CD34, CD79a, CD43, and CD30 as well as slight positivity for TdT and CD20. Bcr-Abl rearrangement was found in interphase tumor cell nuclei by fluorescence in situ hybridization (FISH). Furthermore, a translocation t(14;18)(q32;q21) was amplified by polymerase chain reaction (PCR). Bone marrow (BM) examination showed regular hematopoiesis including a negative FISH analysis for Bcr-Abl and complete donor chimerism. Nested PCR from peripheral blood (PB), but not conventional PCR, was positive for the b3a2 Bcr-Abl transcript. Neither radiation nor intensive chemotherapy was capable of achieving a tumor remission, and the patient died from progressive disease 6 months later. Postmortem examinations showed a shift of immunophenotype with appearance of myeloperoxidase-positive tumor cells and loss of lymphoid antigens. In addition, there were characteristic cytogenetic findings of multiple Ph chromosomes and a clonal loss of P53 tumor suppressor gene. The latter was already deleted before HSCT. We conclude that lymphoid neoplasia occurring in our patient should be interpreted as an extramedullary, very immature blast crisis of CML expressing lymphoid differentiation markers rather than a true de novo NHL.
authors.affiliation: Universitätsklinikum Carl Gustav Carus, Medizinische Klinik und Poliklinik I, Fetscherstrasse 74, 01307 Dresden, Germany. kroschinsky@mk1.med.tu-dresden.de
authors.forename: F
authors.initials: F
authors.lastname: Kroschinsky
authors.forename: K
authors.initials: K
authors.lastname: Friedrich
authors.forename: M
authors.initials: M
authors.lastname: Hanel
authors.forename: B
authors.initials: B
authors.lastname: Mohr
authors.forename: T
authors.initials: T
authors.lastname: Langer
authors.forename: M
authors.initials: M
authors.lastname: Meinhardt
authors.forename: C
authors.initials: C
authors.lastname: Thiede
authors.forename: M
authors.initials: M
authors.lastname: Bornhauser
authors.forename: G
authors.initials: G
authors.lastname: Baretton
authors.forename: G
authors.initials: G
authors.lastname: Ehninger
mesh_terms: D000328:Adult; D001752:Blast Crisis / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D020732:Cytogenetic Analysis; D003937:Diagnosis, Differential; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D016130:Immunophenotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis / Q000473:pathology* / Q000628:therapy; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000235:genetics*; D008297:Male; D023981:Sarcoma, Myeloid / Q000175:diagnosis / Q000473:pathology; D013899:Thoracic Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D014178:Translocation, Genetic; D014184:Transplantation, Homologous