Molecular dissection of a quantitative trait locus: a phenylalanine-to-tyrosine substitution in the transmembrane domain of the bovine growth hormone receptor is associated with a major effect on milk yield and composition.
We herein report on our efforts to improve the mapping resolution of a QTL with major effect on milk yield and composition that was previously mapped to bovine chromosome 20. By using a denser chromosome 20 marker map and by exploiting linkage disequilibrium using two distinct approaches, we provide strong evidence that a chromosome segment including the gene coding for the growth hormone receptor accounts for at least part of the chromosome 20 QTL effect. By sequencing individuals with known QTL genotype, we identify an F to Y substitution in the transmembrane domain of the growth hormone receptor gene that is associated with a strong effect on milk yield and composition in the general population.
Blott
Sarah
S
Department of Genetics, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium.
Kim
Jong-Joo
JJ
Moisio
Sirja
S
Schmidt-Küntzel
Anne
A
Cornet
Anne
A
Berzi
Paulette
P
Cambisano
Nadine
N
Ford
Christine
C
Grisart
Bernard
B
Johnson
Dave
D
Karim
Latifa
L
Simon
Patricia
P
Snell
Russell
R
Spelman
Richard
R
Wong
Jerry
J
Vilkki
Johanna
J
Georges
Michel
M
Farnir
Frédéric
F
Coppieters
Wouter
W
D019943:Amino Acid Substitution; D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping; D006239:Haplotypes; D015810:Linkage Disequilibrium; D008126:Lod Score; D018895:Microsatellite Repeats; D008892:Milk / Q000737:chemistry / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism; D010802:Phylogeny; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci; D011986:Receptors, Somatotropin / Q000235:genetics* / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
D011986:Receptors, Somatotropin; D014443:Tyrosine; D010649:Phenylalanine