Pectins and pectic-oligosaccharides inhibit Escherichia coli O157:H7 Shiga toxin as directed towards the human colonic cell line HT29.
Pectins and pectic-oligosaccharides, as derived by controlled enzymatic hydrolysis, were evaluated for their ability to interfere with the toxicity of Shiga-like toxins from Escherichia coli O157:H7. Both types of material resulted in some degree of protection but this was significantly higher (P>0.01) with the oligosaccharide fractions (giving 90-100% cell survival, compared to 70-80% with the polymer). An effect of methylation on the protective effect was detected with lower degrees being more active. The pectic-oligosaccharides and galabiose, the minimum toxin receptor analogue, were shown to inhibit toxicity and were both protective at 10 mg x ml(-1), but not at lower concentrations.
Olano-Martin
Estibaliz
E
School of Food Biosciences, The University of Reading, PO Box 226, Whiteknights, Reading RG6 6AP, UK.
Williams
Mark R
MR
Gibson
Glenn R
GR
Rastall
Robert A
RA
D004927:Escherichia coli Infections / Q000188:drug therapy*; D019453:Escherichia coli O157 / Q000472:pathogenicity*; D019073:HT29 Cells; D006801:Humans; D009844:Oligosaccharides / Q000494:pharmacology*; D010368:Pectins / Q000494:pharmacology*; D022641:Shiga Toxin 2 / Q000037:antagonists & inhibitors* / Q000633:toxicity; D014774:Virulence
D009844:Oligosaccharides; D022641:Shiga Toxin 2; D010368:Pectins