title: Glutaric acid induces oxidative stress in brain of young rats.
abstract: This study investigated the effects of glutaric acid, which predominantly accumulates in glutaric acidemia type I, on some in vitro parameters of oxidative stress in brain of young rats. We evaluated chemiluminescence, total radical-antioxidant potential (TRAP) and the activities of the antioxidant enzymes catalase, glutathione peroxidase and superoxide dismutase in brain tissue homogenates in the presence of glutaric acid at concentrations ranging from 0.05 to 2.0 mM. The acid significantly increased chemiluminescence (up to 65%) and reduced total radical-antioxidant potential (up to 28%) and glutathione peroxidase activity (up to 46%), without affecting catalase and superoxide dismutase activities. The results provide evidence that glutaric acid induces oxidative stress in vitro in rat brain. If these findings also occur in humans, it is possible that they may contribute to the neuropathology of patients affected by glutaric acidemia type I.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 Anexo, Porto Alegre RS 90035-003, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: de Oliveira Marques
authors.forename: Martine Elisabeth Kienzle
authors.initials: ME
authors.lastname: Hagen
authors.forename: Carolina Didonet
authors.initials: CD
authors.lastname: Pederzolli
authors.forename: Angela Malysz
authors.initials: AM
authors.lastname: Sgaravatti
authors.forename: Karina
authors.initials: K
authors.lastname: Durigon
authors.forename: Carla Giordani
authors.initials: CG
authors.lastname: Testa
authors.forename: Clóvis Milton Duval
authors.initials: CM
authors.lastname: Wannmacher
authors.forename: Angela Terezinha
authors.initials: AT
authors.lastname: de Souza Wyse
authors.forename: Moacir
authors.initials: M
authors.lastname: Wajner
authors.forename: Carlos Severo
authors.initials: CS
authors.lastname: Dutra-Filho
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000187:drug effects / Q000378:metabolism* / Q000503:physiopathology; D001928:Brain Diseases, Metabolic / Q000378:metabolism* / Q000503:physiopathology; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D005977:Glutarates / Q000378:metabolism* / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D008163:Luminescent Measurements; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D005609:Free Radicals; D005977:Glutarates; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C035736:glutaric acid