title: Development of a radioimmunoassay procedure for aflatoxin B1 measurement.
abstract: A radioimmunoassaay (RIA) procedure to measure aflatoxin B(1) (AfB(1)) in agricultural commodities was developed. AfB(1) oxime derivative was synthesized, characterized, and used for preparation of (125)I-labeled AfB(1). Antiaflatoxin B(1) serum was raised in-house using AfB(1)-bovine serum albumin conjugate as immunogen. The assay system was optimized in the range of 0.2-5 ng/mL, using a liquid phase (PEG) as well as a solid phase (coated polystyrene beads) separation system. Inter-assay and intra-assay variations, recovery, and parallelism studies validated the assay. AfB(1) analysis was carried out in nearly 130 samples of different agricultural commodities. The correlation coefficient was determined using commercial ELISA and in-house-developed RIA methods.
authors.affiliation: Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Mumbai 400 085, India.
authors.forename: Aruna
authors.initials: A
authors.lastname: Korde
authors.forename: Usha
authors.initials: U
authors.lastname: Pandey
authors.forename: Sharmila
authors.initials: S
authors.lastname: Banerjee
authors.forename: H D
authors.initials: HD
authors.lastname: Sarma
authors.forename: S
authors.initials: S
authors.lastname: Hajare
authors.forename: Meera
authors.initials: M
authors.lastname: Venkatesh
authors.forename: A K
authors.initials: AK
authors.lastname: Sharma
authors.forename: M R A
authors.initials: MR
authors.lastname: Pillai
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D000383:Agriculture; D002523:Edible Grain / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera / Q000276:immunology; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D008863:Microspheres; D011092:Polyethylene Glycols; D011137:Polystyrenes; D011786:Quality Control; D011863:Radioimmunoassay / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D007106:Immune Sera; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D011137:Polystyrenes; D011092:Polyethylene Glycols; D016604:Aflatoxin B1