[Extraction and purification of acidic mucopolysaccharide from Holothuria atra].
Mucopolyaccharide with molecular weight of 10253 Da was extracted and purified from fresh Holothuria atra Jaeger by means of enzymic and alkaline hydrolysis, potassium acetate and ethanol fractional precipitation. It was tested to be purified ingredient with agarose electrophoresis. The percentage content of galactosamine, glucuronic acid, fucose and sulfate in the mucopolysaccharide was 16.12%, 17.88%, 11.66% and 23.52% respectively.
Tang
X
X
Sun Yat-sen University of Medical Sciences, Guangzhou 510089.
Qiu
P
P
Li
M
M
Su
X
X
Yan
G
G
D000818:Animals; D005643:Fucose / Q000032:analysis; D005688:Galactosamine / Q000032:analysis; D020723:Glucuronic Acid / Q000032:analysis; D006025:Glycosaminoglycans / Q000737:chemistry / Q000302:isolation & purification*; D008419:Materia Medica / Q000737:chemistry*; D008970:Molecular Weight; D012616:Sea Cucumbers / Q000737:chemistry*; D013431:Sulfates / Q000032:analysis
D006025:Glycosaminoglycans; D008419:Materia Medica; D013431:Sulfates; D005643:Fucose; D005688:Galactosamine; D020723:Glucuronic Acid