title: [The observation of proliferative cell nuclear antigen and AgNORs in ocular adnexal lymphoid hyperplasia].
abstract: OBJECTIVE
  To investigate the expression of proliferative cell nuclear antigen and Nuclear Organizer Region in ocular adnexal lymphoid hyperplasia and its histopathologic classification.
  
  
  METHODS
  Using anti-proliferative cell nuclear antigen monoclonal antibody (PC10) and AgNORs method, the proliferative cell in 34 cases of ocular adnexal lymphoid neoplasms (benign lymphoid lesion, BLL, 14 cases; atypical lymphoid hyperplasias, AH, 9 cases; malignant lymphomas, ML, 11 cases) were detected in formalin fixed and paraffin-embeded tissue.
  
  
  RESULTS
  A significant difference between BLL and AH, BLL and ML was observed (PCNA P < 0.001, P < 0.001; AgNORs P < 0.01, P < 0.001); similar result was observed between AH and ML in PCNA index (P < 0.01). But there was not a significant difference in AgNORs count (P > 0.05). The linear regression coefficient between PCNA index and AgNORs number was excellent (0.8693).
  
  
  CONCLUSIONS
  The two methods may be helpful to distinguish ocular adnexal lymphoid reactive hyperplasia and malignant lymphoma.
authors.affiliation: Department of Ophthalmology, Changzheng Hospital, Second Military University, Shanghai 200003, China.
authors.forename: R
authors.initials: R
authors.lastname: Wei
authors.forename: T
authors.initials: T
authors.lastname: Ye
authors.forename: J
authors.initials: J
authors.lastname: Cai
authors.forename: H
authors.initials: H
authors.lastname: Zhu
authors.forename: S
authors.initials: S
authors.lastname: Xi
mesh_terms: D034961:Antigens, Nuclear; D005128:Eye Diseases / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008223:Lymphoma / Q000378:metabolism / Q000473:pathology; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism; D009697:Nucleolus Organizer Region / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D019310:Pseudolymphoma / Q000378:metabolism* / Q000473:pathology; D016622:Silver Staining
chemical_list: D034961:Antigens, Nuclear; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; C051963:nucleolar organizer region associated proteins