[The expression of glucocorticold receptor isoform in peripheral blood mononuclear cell with Graves' ophthalmopathy].
OBJECTIVE
To study the expression of glucocorticoid receptor (GR) isoform in the peripheral blood mononuclear cell (PBMC) of patients with Graves' ophthalmopathy (GO).


METHODS
Semi-quantitative RT-PCR was used to analyze RNA isolated from PBMC of the patients with GO and the normal volunteers. The level of plasma total cortisol (PTC) at 8 a.m. was measured.


RESULTS
The hGR alpha/GR beta mRNA ratios of the two groups were 7.58 +/- 5.42 and 14.65 +/- 8.30, respectively. There was significant difference between the two groups (P < 0.05). The PTC levels of the two groups were 304.23 +/- 92.06 and 313.73 +/- 111.05, and no significant difference between them was noted (P > 0.05). No correlation was found between hGR alpha/GR beta mRNA and PTC levels.


CONCLUSIONS
hGR alpha/GR beta mRNA may play a role in the pathogeny of Graves' ophthalmopathy.
An
Zhenmei
Z
Department of Endocrinology, West China Hospital, Sichuan University, Chengdu 610041, China.
Yu
Nan
N
Wei
Songquan
S
Xia
Qingjie
Q
Li
Shuangqing
S
Xie
Qin
Q
D000328:Adult; D005260:Female; D006111:Graves Disease / Q000097:blood*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D020033:Protein Isoforms / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis* / Q000235:genetics
D020033:Protein Isoforms; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D006854:Hydrocortisone