[Effect of wuzi yanzong Pill on mitochondrial DNA deletion and respiratory chain enzyme complex activity in peripheral leukocyte of aged male with kidney deficiency syndrome].
OBJECTIVE
To investigate the effect of Wuzi Yanzong Pill (WZYZP) on mitochondrial DNA (mtDNA) deletion and respiratory chain enzyme complex (RCZC) in peripheral blood leukocyte of aged male with Kidney Deficiency Syndrome (KDS).


METHODS
Single-blinded study was conducted in 38 aged male with KDS, who were randomly divided into 2 groups treated with WZYZP and placebo respectively for 3 months. Levels of mtDNA deletion and RCZC were determined by polymerase chain reaction (PCR) and enzyme kinetics technique respectively.


RESULTS
WZYZP could reduce the mtDNA deletion and raise the activity of mitochondrial RCZC I, IV in peripheral blood leukocyte of aged male with KDS (P < 0.05, P < 0.01).


CONCLUSIONS
WZYZP has protective effect on mtDNA from oxidative damage in leukocyte of aged male with KDS.
Wang
Xue-mei
XM
Institute of Integrated Traditional Chinese and Western Medicine, Peking University, Beijing 100034.
Fu
Hong
H
Liu
Geng-xin
GX
D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000187:drug effects*; D004272:DNA, Mitochondrial / Q000235:genetics*; D003937:Diagnosis, Differential; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006801:Humans; D007674:Kidney Diseases / Q000188:drug therapy; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008516:Medicine, Chinese Traditional; D008875:Middle Aged; D008517:Phytotherapy; D017384:Sequence Deletion / Q000187:drug effects; D016711:Yang Deficiency / Q000188:drug therapy* / Q000235:genetics
D004272:DNA, Mitochondrial; D004365:Drugs, Chinese Herbal