title: Molecular diagnosis of infective endocarditis by PCR amplification and direct sequencing of DNA from valve tissue.
abstract: We used broad-range eubacterial PCR amplification followed by direct sequencing to identify microbial pathogens in heart valve material from 29 patients with histologically confirmed infective endocarditis and 23 patients free of infective endocarditis. Microorganisms cultured by conventional techniques matched those identified by PCR in 21 cases. PCR alone identified the causative agent in three cases (Streptococcus bovis, Staphylococcus cohnii, and Coxiella burnetii), allowing better patient management. PCR corrected the initial bacteriological diagnosis in three cases (Streptococcus bovis, Streptococcus mutans, and Bartonella henselae). Among the 29 cases of histologically confirmed infective endocarditis, PCR findings were positive in 27 cases and were consistent with the bacterial morphology seen at Gram staining (26 cases) or with the results obtained by immunohistologic analysis with an anti-C. burnetii monoclonal antibody (one case). In two other cases of histologically confirmed infective endocarditis, PCR remained negative in a blood culture-negative case for which no bacteria were seen at histological analysis and in one case with visualization of cocci and blood cultures positive for Enterococcus faecalis. Ten clinical diagnoses of possible infective endocarditis were ruled out by histopathological analysis of the valves and subsequently by PCR. PCR was negative in 13 of the 14 patients in whom infective endocarditis was rejected on clinical grounds; the other patient was found to have Coxiella burnetii infective endocarditis on the basis of PCR and histopathological analysis and was subsequently included in the group of 29 definite cases. In total, PCR contributed to the diagnosis and management of infective endocarditis in 6 of 29 (20%) cases.
authors.affiliation: Laboratoire de Bactériologie, Hôpital Louis Pradel, 69394 Lyon Cedex 03, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Gauduchon
authors.forename: Lara
authors.initials: L
authors.lastname: Chalabreysse
authors.forename: Jerome
authors.initials: J
authors.lastname: Etienne
authors.forename: Marie
authors.initials: M
authors.lastname: Célard
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Benito
authors.forename: Hubert
authors.initials: H
authors.lastname: Lepidi
authors.forename: Françoise
authors.initials: F
authors.lastname: Thivolet-Béjui
authors.forename: François
authors.initials: F
authors.lastname: Vandenesch
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis; D004697:Endocarditis, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D006351:Heart Valves / Q000382:microbiology*; D006801:Humans; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S