title: Calcifying odontogenic cyst: clinicopathological features and immunohistochemical profile of 10 cases.
abstract: BACKGROUND
  Calcifying odontogenic cyst (COC) is an uncommon odontogenic lesion with few studies describing its immunohistochemical profile and proliferative activity reported in the literature.
  
  
  METHODS
  Clinical and histological features and immunohistochemical expression of cytokeratins, Mel-CAM (CD146), bcl-2, PCNA and ki-67, in 10 cases of COC were studied.
  
  
  RESULTS
  All 10 cases affected the maxilla, eight intraosseous and two peripheral. Five central cases were cystic and three were cystic associated with odontoma, and the two extraosseous showed solid histological pattern; immunohistochemistry was positive for cytokeratins 8, 14, 19, AE1/AE3 and 34betaE12 and bcl-2 in all cases, and Mel-CAM in six cases. Proliferative activity was greater in the epithelium of central cystic COC in relation to COC associated with odontoma and peripheral lesions.
  
  
  CONCLUSIONS
  Calcifying odontogenic cysts showed odontogenic cytokeratin profile and bcl-2 and Mel-CAM expression indicate that these proteins may be involved in the development of COC. There were no recurrences after surgery, irrespective of their proliferative activity.
authors.affiliation: Oral Pathology, School of Dentistry of Piracicaba, State University of Campinas, Piracicaba/SP, Brazil.
authors.forename: Eduardo Rodrigues
authors.initials: ER
authors.lastname: Fregnani
authors.forename: Fábio Ramôa
authors.initials: FR
authors.lastname: Pires
authors.forename: Rivera Daniel
authors.initials: RD
authors.lastname: Quezada
authors.forename: Ie Ming
authors.initials: IeM
authors.lastname: Shih
authors.forename: Pablo Agustin
authors.initials: PA
authors.lastname: Vargas
authors.forename: Oslei Paes
authors.initials: OP
authors.lastname: de Almeida
mesh_terms: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D051929:CD146 Antigen; D002455:Cell Division; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis / Q000145:classification; D019394:Ki-67 Antigen / Q000032:analysis; D008441:Maxillary Neoplasms / Q000473:pathology*; D008562:Membrane Glycoproteins / Q000032:analysis; D009378:Neoplasms, Multiple Primary / Q000473:pathology; D019006:Neural Cell Adhesion Molecules; D018333:Odontogenic Cyst, Calcifying / Q000473:pathology*; D009810:Odontoma / Q000473:pathology; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D014408:Biomarkers, Tumor; D051929:CD146 Antigen; D019394:Ki-67 Antigen; C497902:MCAM protein, human; D008562:Membrane Glycoproteins; D019006:Neural Cell Adhesion Molecules; D018809:Proliferating Cell Nuclear Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D007633:Keratins