Fabrication and evaluation of a new composite composed of tricalcium phosphate, gelatin and chi-li-saan as a bone substitute.
The purpose of this study was to prepare and evaluate the feasibility and biocompatibility of a new composite as a bone substitute. The new composite (GTGC) was mainly composed of tricalcium phosphate ceramics and gelatin to which chi-li-saan, a Chinese medicinal remedy was added. The GTGC composite was manually packed into cylindrical Teflon molds, dried overnight in an oven and sterilized by gamma-ray prior to use. Mature New Zealand rabbits, weighting 3-3.5 kg, underwent full-thickness excision of the parietal bone. In the experimental group, bone defects of 12 animals were filled with the GTGC composites and another 12 unreconstructed rabbits were considered as controls. Three rabbits were examined for each group in every time period at 2, 4, 8 and 12 weeks after operation. There was no evidence of adverse tissue reaction to the GTGC composite. In addition, examination with light and fluorescent microscopy revealed a significantly greater amount of new bone ingrowth in the GTGC group at the same implantion time as compared with the controls. Therefore, the GTGC composite could serve as a useful substitute when repairing bone defects.
Yao
Chun-Hsu
CH
Department of Radiological Technology Chung-Tai Institute of Health Science and Technology, Taichung, Taiwan, ROC. chyao@ctc.edu.tw
Tsai
Chin-Chuan
CC
Chen
Yueh-Sheng
YS
Chang
Chen-Jung
CJ
Liu
Bai-Shuan
BS
Lin
Chun-Ching
CC
Tsuang
Yang-Hwei
YH
D000818:Animals; D001672:Biocompatible Materials; D001846:Bone Development / Q000187:drug effects; D018786:Bone Substitutes / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology; D002130:Calcium Phosphates / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology; D005079:Excipients; D005592:Fracture Fixation; D050723:Fractures, Bone / Q000000981:diagnostic imaging / Q000473:pathology; D005780:Gelatin / Q000009:adverse effects / Q000737:chemistry*; D011817:Rabbits; D011859:Radiography; D012886:Skull / Q000000981:diagnostic imaging / Q000473:pathology
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D004365:Drugs, Chinese Herbal; D005079:Excipients; C485817:beta-tricalcium phosphate; C473659:chi-li-saan; D005780:Gelatin; C018392:tricalcium phosphate