title: Canine Bcl-xL gene and its expression in tumor cell lines.
abstract: The canine Bcl-xL gene was cloned and sequenced. Canine Bcl-xL cDNA clone was 1252 bp in length, and encoded 233 deduced amino acides. The predicted canine Bcl-xL amino acid sequence shared 99.6%, 97.0%, 97.9%, 98.7% and 98.3% homology with that of human, mouse, rat, sheep and pig Bcl-xL, respectively. RT-PCR analysis revealed that canine Bcl-xL mRNA was constitutively expressed in CL-1 (canine lymphoma) and GL-1 (canine B cell leukemia) cell lines.
authors.affiliation: Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Sano
authors.forename: Keisuke
authors.initials: K
authors.lastname: Oguma
authors.forename: Rui
authors.initials: R
authors.lastname: Kano
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hasegawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004283:Dog Diseases / Q000235:genetics; D004285:Dogs / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D007938:Leukemia / Q000235:genetics / Q000662:veterinary; D008223:Lymphoma / Q000235:genetics / Q000662:veterinary; D008969:Molecular Sequence Data; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured; D051020:bcl-X Protein
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein