title: Isolation of a U-insertion/deletion editing complex from Leishmania tarentolae mitochondria.
abstract: A multiprotein, high molecular weight complex active in both U-insertion and U-deletion as judged by a pre-cleaved RNA editing assay was isolated from mitochondrial extracts of Leishmania tarentolae by the tandem affinity purification (TAP) procedure, using three different TAP-tagged proteins of the complex. This editing- or E-complex consists of at least three protein-containing components interacting via RNA: the RNA ligase-containing L-complex, a 3' TUTase (terminal uridylyltransferase) and two RNA-binding proteins, Ltp26 and Ltp28. Thirteen approximately stoichiometric components were identified by mass spectrometric analysis of the core L-complex: two RNA ligases; homologs of the four Trypanosoma brucei editing proteins; and seven novel polypeptides, among which were two with RNase III, one with an AP endo/exonuclease and one with nucleotidyltransferase motifs. Three proteins have no similarities beyond kinetoplastids.
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics, Howard Hughes Medical Institute, University of California at Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Ruslan
authors.initials: R
authors.lastname: Aphasizhev
authors.forename: Inna
authors.initials: I
authors.lastname: Aphasizheva
authors.forename: Robert E
authors.initials: RE
authors.lastname: Nelson
authors.forename: Guanghan
authors.initials: G
authors.lastname: Gao
authors.forename: Agda M
authors.initials: AM
authors.lastname: Simpson
authors.forename: Xuedong
authors.initials: X
authors.lastname: Kang
authors.forename: Arnold M
authors.initials: AM
authors.lastname: Falick
authors.forename: Sandro
authors.initials: S
authors.lastname: Sbicego
authors.forename: Larry
authors.initials: L
authors.lastname: Simpson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D007891:Leishmania / Q000235:genetics* / Q000378:metabolism; D008928:Mitochondria / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011485:Protein Binding; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017393:RNA Editing; D016415:Sequence Alignment; D014498:Uracil
chemical_list: D015800:Protozoan Proteins; D014498:Uracil