[Culture of human nasal respiratory epithelial cells in serum free medium supplemented with hormones and growth factors and measurement of human nasal ciliary motility using videomicroscopy].
OBJECTIVE
To establish a culture model of human nasal respiratory epithelial cells and a method of measuring human nasal ciliary motility.


METHODS
The human nasal respiratory epithelial cells were detached with collagenase and cultured in serum free medium supplemented with hormones and growth factors, the ciliary beat frequency was measured by videomicroscopy.


RESULTS
After inoculation, cells cultured with this method adhered in 24 hours, confluented in 6-8 days and lived for 16 days. During that time ciliary beating was active, both acidic and neutral mucoitin granules were rich in goblet cells and all chromosome of 23 pairs were normal, the ciliary beat frequency in 29 subjects' nasal mucosa was (411 +/- 24) beats/min (mean +/- s).


CONCLUSIONS
A culture model of human nasal respiratory epithelial cells in serum free medium supplemented with hormones and growth factors and a method of measuring human nasal ciliary motility was successfully established.
Wang
F
F
Department of Otorhinolaryngology, PLA 304 Hospital, Beijing 100037.
Su
Z L
ZL
Huang
J X
JX
Wang
L
L
Yang
W Y
WY
Jiang
S C
SC
D002478:Cells, Cultured; D002923:Cilia / Q000502:physiology* / Q000648:ultrastructure; D016895:Culture Media, Serum-Free; D016228:Endothelial Growth Factors / Q000494:pharmacology; D004847:Epithelial Cells / Q000502:physiology*; D006728:Hormones / Q000494:pharmacology; D006801:Humans; D008854:Microscopy, Electron; D009297:Nasal Mucosa / Q000166:cytology*
D016895:Culture Media, Serum-Free; D016228:Endothelial Growth Factors; D006728:Hormones