[Extraction of RNA from Stored Bone Marrow Smears for Detecting Expression of the WT1 Gene in MDS Patients]
RNA was extracted from stored bone marrow smears, expression of WT1 gene in the patients with MDS was examined by means of nest RT-PCR. The results showed WT1 gene was highly expressed in some cases of MDS. Expression rates were higher in the patients with RAEB and RAEB-t than those patients with RA and RAS. WT1 expression is related to the advance of MDS. The detection of WT1 gene expression by RT-PCR might be useful for assessing disease progress in the patients with MDS.
Wang
Rong
R
Department of Hematology, Medical College, Jinan University, Guangzhou 510632, China; Ruian People's Hospitol, Zhejiang Province, China.
Po
Xi-Yan
XY
Yu
Zhi
Z
Chen
Zhi-Shong
ZS
Zhang
Yuan
Y
Yu
Zhi-Fei
ZF