Differential secretion of cytokines and adhesion molecules by HUVEC stimulated with low concentrations of bleomycin.
Bleomycin (BLM) is known to induce lung inflammation and subsequent fibrosis. Endothelial cells have been reported to play an important role, producing cytokines and adhesion molecules during the inflammatory process in pulmonary fibrosis. To examine the effects of BLM on endothelial cells, we investigated the expression profiles of various cytokines and adhesion molecules produced by endothelial cells stimulated with BLM. Increased expressions of interleukin-8 and monocyte chemoattractant protein-1 measured as protein as well as mRNA by human umbilical vein endothelial cells (HUVECs) were detected after exposure to BLM. Similarly, increased expressions of E-selectin and intercellular adhesion molecule-3 were detected both at the protein and mRNA levels. Under these conditions, a small but significant decrease of [3H]thymidine uptake was detected. These findings indicate that HUVEC were stimulated to secrete cytokines and express adhesion molecules in the presence of low concentrations of BLM which have a mildly inhibitory effect on cellular proliferation.
Miyamoto
Haruko
H
Fourth Department of Internal Medicine, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan. hmiyamo@mte.biglobe.ne.jp
Sugawara
Isamu
I
Azuma
Arata
A
Saito
Yoshinobu
Y
Kohno
Nobuoki
N
Kudoh
Shoji
S
D001761:Bleomycin / Q000494:pharmacology*; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000096:biosynthesis*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000032:analysis / Q000096:biosynthesis; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D019040:E-Selectin / Q000032:analysis / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000032:analysis / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014471:Umbilical Veins
D015815:Cell Adhesion Molecules; D018932:Chemokine CCL2; D016207:Cytokines; D019040:E-Selectin; D016209:Interleukin-8; D012333:RNA, Messenger; D001761:Bleomycin; D013936:Thymidine