[Preparation of liposome encapsulated daunorbicine and determination of daunorubicine of aqueous humor in rabbit eyes].
OBJECTIVE
To investigate the method of determination the concentration of daunorubicine(DNR) and liposome encapsulated daunorubicine(LDNR) of aqueous humor in rabbit eyes.


METHODS
After extracapsular lens extraction, 0.1 ml of 1 mg/ml DNA and LDNR were injected into the anterior chamber. At the 12, 24, 48 hours and 1 week after operation, the aqueous humor were aspirated to determine the concentration of DNR by high performance liquid chromatography(HPLC).


RESULTS
At the 12, 24, 48 hours after operation, DNR were determined in all eyes, but at one week after operation, the DNR could only be detected in LDNR group. The concentration of DNR in LDNR group were higher than DNR group.
Wu
M
M
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Li
H
H
Li
S
S
Pan
S
S
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D001682:Biological Availability; D002386:Cataract / Q000209:etiology / Q000517:prevention & control*; D002387:Cataract Extraction / Q000009:adverse effects*; D002851:Chromatography, High Pressure Liquid; D003630:Daunorubicin / Q000032:analysis / Q000493:pharmacokinetics*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005260:Female; D008081:Liposomes / Q000032:analysis / Q000493:pharmacokinetics*; D008297:Male; D011184:Postoperative Period; D011817:Rabbits
D004337:Drug Carriers; D008081:Liposomes; D003630:Daunorubicin