[Detection of cytomegalovirus antigens and virus DNA in the peripheral blood after organ transplantation].
OBJECTIVE
To diagnose timely the cytomegalovirus(CMV) infection of recipients after organ transplantation.


METHODS
Thirty two organ transplanted recipients were demonstrated for CMV infection based on detection of antigenemia by immunoperoxidase assay, viremia with virus isolation, DNAemia by polymerase chain reaction (PCR) and in situ hybridization (ISH) in peripheral blood samples.


RESULTS
Of 89 blood specimens examined, 35(39.3%) were positive for antigenemia, 25(28.1%) for viremia, 37(41.6%) for DNAemia by ISH, and 51(57.3%) for DNAemia by PCR. The results indicated that DNAemia assay with ISH and PCR and antigenemia assay were suitable methods for rapid, early, and sensitive detection of CMV infection. The results correlated with clinical symptoms.


CONCLUSIONS
We suggest that detection of antigenemia, viremia, and DNAemia can monitor CMV infection of organ transplaned recipients and provide indication to antiviral therapy.
Xiao
Y
Y
Department of Microbiology and Virology, Hunan Medical University, Changsha 410078.
Shi
K
K
Mao
X
X
D000328:Adult; D000956:Antigens, Viral / Q000097:blood*; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D003587:Cytomegalovirus / Q000276:immunology* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000821:virology; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D014766:Viremia / Q000821:virology
D000956:Antigens, Viral; D004279:DNA, Viral