title: [Effect of liposome on permeability and drug retention of sodium fluorescein in rat skin: an experimental study].
abstract: OBJECTIVE
  To investigate the effect of liposome on the permeability and drug retention of sodium fluorescein(NaFl) in rat skin.
  
  
  METHODS
  With an improved Franz diffusion cell and 0.125% NaFl liposome suspension as the model drug, in vitro measurement of percutaneous absorption and skin reservoir capacity for NaF1 was conducted using a fluorescence spectrophotometry at 4 and 12 h respectively after the diffusion experiment had started, and distribution of the fluorescence in rat skin was observed under fluorescence microscope at 4 h penetration experiment. NaFl solution of the same concentration as the model drugs served as the control for this experiment.
  
  
  RESULTS
  In comparison with NaFl solution, liposomal NaFl suspension resulted in larger amount of NaFl retention in rat skin but smaller amount in the receiver cell in a four-hour Franz diffusion test, with also higher fluorescence intensity in the skin, especially the skin around the hair follicles.
  
  
  CONCLUSIONS
  Local high drug concentrations can be achieved in the skin by liposomal suspension of water soluble drug for more effective treatment of skin diseases.
authors.affiliation: Department of Dermatology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. shengyu@sohu.com
authors.forename: Yu-sheng
authors.initials: YS
authors.lastname: Xie
authors.forename: Kang
authors.initials: K
authors.lastname: Zeng
authors.forename: Guo-feng
authors.initials: GF
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Dong
authors.forename: Yi-min
authors.initials: YM
authors.lastname: Shi
authors.forename: Xiao-liang
authors.initials: XL
authors.lastname: Zhu
authors.forename: Le-dong
authors.initials: LD
authors.lastname: Sun
authors.forename: Gui-rong
authors.initials: GR
authors.lastname: Hou
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D003287:Contrast Media / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D004337:Drug Carriers; D016503:Drug Delivery Systems; D019793:Fluorescein / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D008081:Liposomes / Q000737:chemistry*; D010539:Permeability; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption
chemical_list: D003287:Contrast Media; D004337:Drug Carriers; D008081:Liposomes; D019793:Fluorescein