[Studies on immunoprotection in mice after immunization with Schistosoma japonicum 22.6 kDa recombinant protein].
OBJECTIVE
To evaluate the immunoprotective effect of Schistosoma japonicum recombinant 22.6 kDa (rSj22.6) and Sj22.6/Sj26 GST fusion protein.


METHODS
The Sj22.6/Sj26 GST fusion protein was prepared by affinity chromatography using glutathione Sepharose 4B. The purified rSj22.6 could be cleaved easily from the fusion protein with Thrombin. 17 and 12 mice immunized with rSj22.6 and Sj22.6/Sj26 GST separately were each challenged with 40 +/- 1 S. japonicum cercariae.


RESULTS
In BALB/c mice, the rSj22.6 and Sj22.6/Sj26 GST could induce 32.1 (P < 0.005) and 34.9% (P < 0.02) worm reduction, respectively, as well as 28.4% (P < 0.02) and 45.1% (P < 0.005) total egg reduction, respectively.


CONCLUSIONS
Bpth rSj22.6 and Sj22.6/Sj26 GST fusion protein are partially effective against S. japonicum.
Su
C
C
Department of Parasitology, Nanjing Medical University, Nanjing 210029.
Ma
L
L
Wang
R
R
Hu
X
X
Chen
S
S
Shao
L
L
Wu
H
H
Shen
L
L
Zhang
Z
Z
Wu
G
G
D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D015801:Helminth Proteins / Q000276:immunology; D007114:Immunization; D007167:Immunotherapy; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011993:Recombinant Fusion Proteins / Q000627:therapeutic use; D011994:Recombinant Proteins / Q000276:immunology / Q000627:therapeutic use*; D012552:Schistosomiasis / Q000517:prevention & control*; D012554:Schistosomiasis japonica / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014611:Vaccination; D019444:Vaccines, DNA / Q000627:therapeutic use
D000947:Antigens, Helminth; D015801:Helminth Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D019444:Vaccines, DNA