[Inducible expression of MSP1 gene of Plasmodium falciparum by a tetracycline-controlled promoter].
OBJECTIVE
To express the entire MSP1 gene of Plasmodium falciparum and its C-terminal 42 kDa fragment using a tetracycline-controlled PLtetO-1 promoter.


METHODS
The entire MSP1 gene and 42 kDa fragment gene were cloned into the plasmid of pZE11, and transformed into E. coli DH5 alpha Z1. Restriction enzyme analysis, SDS-PAGE and Western blotting were used to examine two recombinant plasmids and their expression in E. coli DH5 alpha Z1.


RESULTS
The recombinant plasmids of pZE11/MSP1 and pZE11/MSP1-42 were constructed successfully. The expressive products about 190 kDa and 42 kDa of two genes in E. coli DH5 alpha Z1 were identified by SDS-PAGE and Western blotting.


CONCLUSIONS
Tightly controlling expression of the MSP1 gene in E. coli is essential to reduce the toxicity of the product to its host cells as well as to provide a feasibility to construct Salmonella vaccine strain which can inducibly express the important malarial vaccine candidate gene.
Qian
F
F
Department of Etiological Biology, Second Military Medical University, Shanghai 200433.
Pan
W Q
WQ
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D020066:Merozoite Surface Protein 1 / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D010963:Plasmodium falciparum / Q000235:genetics*; D011401:Promoter Regions, Genetic; D013752:Tetracycline / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D020066:Merozoite Surface Protein 1; D013752:Tetracycline