[The role of progesterone in the regulation of gene expression of insulin-like growth factor-I receptor in human decidual stromal cells of early pregnancy in vitro].
OBJECTIVE
To assess the effect of progesterone on the gene expression of insulin-like growth factor-I receptor (IGF-I R) in human decidual stromal cells of early pregnancy in vitro.


METHODS
Semiquantitative reverse transcriptase polymerase chain reaction, using beta-ACTIN as internal standard, was applied to determine the levels of IGF-I R mRNA in human decidual stromal cells of early pregnancy in vitro after cultured with different concentrations of progesterone for 72 hours or cultured with 0.1 mumol/L of progesterone for different periods of time.


RESULTS
The expression of IGF-I R mRNA was significantly positive in human decidual stromal cells of early pregnancy in vitro. The levels of IGF-I R mRNA were down-regulated by progesterone, and showed significant negative-correlation with the concentration of progesterone (r = -0.680, P < 0.001). The levels of IGF-I R mRNA showed no significant correlation with time when the final concentration of progesterone was 0.1 mumol/L (r = 0.005, P > 0.05).


CONCLUSIONS
Progesterone may play an important role in the regulation of proliferation and decidualization of stromal cells by down-regulating the expression of IGF-I R mRNA in human decidual stromal cells of early pregnancy, which is important for the maintenance of early pregnancy.
Li
Shangwei
S
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Li
Lei
L
Cao
Zeyi
Z
Peng
Zhilan
Z
Han
Ziyan
Z
Yang
Yezhou
Y
D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003656:Decidua / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011374:Progesterone / Q000494:pharmacology*; D011897:Random Allocation; D017526:Receptor, IGF Type 1 / Q000096:biosynthesis* / Q000235:genetics; D017154:Stromal Cells / Q000378:metabolism
D011374:Progesterone; D017526:Receptor, IGF Type 1