Influence of carboxymethyl dextran and ferric citrate on the adhesion of CHO cells on microcarriers.
Due to the inherent risks of animal-derived raw materials, the biopharmaceutical industry has an increasing demand for serum-free and protein-free media for industrial cell culture bioprocesses. The absence of serum often changes the characteristics of mammalian cells, especially growth, productivity, and adherence properties. This study is mainly focused on the influence of media additives on cell adherence characteristics. An array of different carboxymethyl dextrans and different ferric citrate concentrations was tested with a number of CHO clones, using standard cell culture Roux-flasks and Cytoline 1 macroporous microcarriers. A prototype mixing system with controlled shear force input was developed as a screening system for adherence characteristics. The results of this evaluation revealed a negatively correlated dose-dependent influence on adhesion for ferric citrate. It was also found that certain carboxymethyl dextrans are capable of increasing the adherence on Roux-flasks and microcarriers.
Landauer
Karlheinz
K
Institute of Applied Microbiology, Muthgasse 18/Haus B, 1190 Wien, Austria. Karlheinz.Landauer@blackbox.net
Wiederkum
Susanne
S
Dürrschmid
Markus
M
Klug
Helga
H
Simic
Gordana
G
Blüml
Gerald
G
Doblhoff-Dier
Otto
O
D000818:Animals; D019149:Bioreactors / Q000145:classification / Q000382:microbiology*; D016466:CHO Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D006224:Cricetinae; D003470:Culture Media / Q000494:pharmacology; D003911:Dextrans / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005290:Ferric Compounds / Q000494:pharmacology*; D008567:Membranes, Artificial
D003470:Culture Media; D003911:Dextrans; D005290:Ferric Compounds; D008567:Membranes, Artificial; C025314:ferric citrate; C014392:carboxymethyl dextran