[Expression of non-structural region 3 gene of the Chinese HGY and analysis of the antigenicity of the recombinant proteins].
OBJECTIVE
This study is to analyze the antigenicity of the NS3 proteins of Chinese HGV and their potential use in the serological diagnosis.


METHODS
All three gene fragments of NS3 region of Chinese HGV were cloned into the pRSET vectors to construct recombinant plasmids. In E. coli BL21, all three recombinant plasmids achieved a high expression level with induction of IPTG. The expressed products were analyzed with Western blot and ELISA.


RESULTS
The recombinant protein PA, P3 and P4 have a molecular weight of 42,000, 30,000 and 24,000, respectively. They all could react with HGV positive sera in Western blot and ELISA. Among them, the protein that covers the N terminal of NS3 region of HGV had a stronger reaction with HGV positive sera than the other two proteinsdid.


CONCLUSIONS
The N terminal in the NS3 region of Chinese HGV includes an dominant antigenic determinant, and its gene product has relatively strong antigenicity.
Chen
G
G
Institute of Virology, Chinese Academy of Preventive Medicine 100052.
Bi
S
S
Zhan
M
M
D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D010957:Plasmids / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D020365:RNA Helicases; D012367:RNA, Viral / Q000032:analysis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D020365:RNA Helicases; D012367:RNA, Viral; D011994:Recombinant Proteins; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases