Lactofen induces isoflavone accumulation and glyceollin elicitation competency in soybean.
Lactofen, the active ingredient of the soybean disease resistance-inducing herbicide, Cobra, induces large accumulations of isoflavone conjugates and aglycones in soybean tissues. The predominant isoflavones induced in cotyledon tissues are daidzein (and its conjugates) and formononetin and glycitein aglycones. The latter two isoflavones are usually present only at very low levels in soybean seedling tissues. In leaves, the predominant lactofen-induced isoflavones are daidzein and formononetin aglycones and the malonyl-glucosyl conjugate of genistein. Isoflavone induction also occurs in cells distal to the point of treatment, but is only weakly systemic. Lactofen also induces elicitation competency, the capacity of soybean cells to accumulate the pterocarpan phytoalexin glyceollin in response to glucan elicitors from the cell wall of the pathogen Phytophthora sojae. Comparison of the activity of a series of diphenyl ether herbicides demonstrated that while all diphenyl ethers tested induced some degree of elicitation competency, only certain ones induced isoflavone accumulation in the absence of glucan elicitor. As a group the diphenyl ethers are thought to inhibit protoporhyrinogen oxidase, eventually leading to singlet oxygen generation. Another singlet oxygen generator, rose bengal, also induced elicitation competency, but little isoflavone accumulation. It is hypothesized that diphenyl ether-induced activated oxygen species mimic some aspects of hypersensitive cell death, which leads to elicitation competency in infected tissues.
Landini
Serena
S
Department of Plant Pathology, The Ohio State University, Columbus, OH 43210, USA.
Graham
Madge Y
MY
Graham
Terrence L
TL
D001578:Benzopyrans / Q000378:metabolism*; D018548:Cotyledon / Q000187:drug effects / Q000378:metabolism; D055768:Halogenated Diphenyl Ethers; D006540:Herbicides / Q000378:metabolism / Q000494:pharmacology; D007529:Isoflavones / Q000378:metabolism*; D015394:Molecular Structure; D010647:Phenyl Ethers / Q000378:metabolism / Q000494:pharmacology*; D010935:Plant Diseases; D036343:Pterocarpans; D012395:Rose Bengal / Q000494:pharmacology; D013025:Glycine max / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D013997:Time Factors
D001578:Benzopyrans; D055768:Halogenated Diphenyl Ethers; D006540:Herbicides; D007529:Isoflavones; D010647:Phenyl Ethers; D036343:Pterocarpans; D012395:Rose Bengal; C017343:glyceollin; C055370:lactofen