A novel putative collaborator of p19ARF.
We performed a yeast interactive screen in search of p19(ARF)-binding partners and have isolated a novel serine rich protein that is assigned to human chromosome 4q35 and mouse chromosome 8. The human and mouse proteins showed 84.2% homology. It is named CARF for its role as a putative Collaborator of ARF. CARF binds to both mouse and human ARF proteins. Its expression was detected in a variety of human tissues. The cDNA was expressed in bacteria and mammalian cells as a soluble and predominantly nucleoplasmic protein, respectively. CARF is a novel binding partner of ARF and might be involved in its p53-dependent or -independent tumor suppressive functions.
Wadhwa
Renu
R
Gene Function Research Laboratory, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Sugihara
Takashi
T
Hasan
Md Kamrul
MK
Duncan
Emma L
EL
Taira
Kazunari
K
Kaul
Sunil C
SC
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics*; D033481:Chromosomes, Mammalian / Q000235:genetics; D019941:Cyclin-Dependent Kinase Inhibitor p16; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D015003:Yeasts
C417743:Cdkn2a protein, mouse; D019941:Cyclin-Dependent Kinase Inhibitor p16; D018076:DNA, Complementary; D025803:Tumor Suppressor Protein p14ARF