[The detection and analysis of human papillomavirus in infantile respiratory tract papillomas by PCR/RFLP study].
OBJECTIVE
To study the relationship between human papillomavirus (HPV) and pathological characteristics of the patients with infantile respiratory tract papillomas (IRP).


METHODS
Thirteen cases of infantile respiratory tract papillomas tissue were analysed for Papillomavirus by the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).


RESULTS
The amplified products of the HPV-L1 region by PCR by using the consensus primers showed that the presence of HPV was 100 percent. Eight cases(61.5 percent) were positive for HPV6 only. Four cases (30.8 percent) were positive for HPV11 only. One case (7.7 percent) showed the presence of HPV18. None were positive for HPV16, 31, 33, 52, 58.


CONCLUSIONS
Infantile respiratory tract papillomas are associated with HPV6, 11, 18 infection, but the subtypes of HPV are not associated with clinical pathological characteristics.
Fu
M
M
Department of Otolaryngology, Guangdong Provincial People's Hospital, Guangzhou 510080.
Liu
S B
SB
Wang
D Y
DY
Hou
F L
FL
Li
Q L
QL
D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007223:Infant; D007822:Laryngeal Neoplasms / Q000601:surgery / Q000821:virology*; D008297:Male; D010212:Papilloma / Q000601:surgery / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014412:Tumor Virus Infections
D004279:DNA, Viral