title: Affinity-capture reagents for protein arrays.
abstract: The simultaneous identification and quantitative measurement of the production levels of thousands of different proteins in a biological specimen remains an unachieved goal of modern proteomic research. Advances in the development of microarray-based platforms for highly parallel detection of proteins have therefore received a considerable impulse during the last few years. Here, we review the existing reagents for affinity capture of protein targets, as well as the techniques used for their immobilization on solid supports and methods for the detection of binding events, underlining the problems and the opportunities in this continuously evolving research field.
authors.affiliation: Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland. giueli@pharma.anbi.ethz.ch
authors.forename: Giuliano
authors.initials: G
authors.lastname: Elia
authors.forename: Michela
authors.initials: M
authors.lastname: Silacci
authors.forename: Simone
authors.initials: S
authors.lastname: Scheurer
authors.forename: Jörg
authors.initials: J
authors.lastname: Scheuermann
authors.forename: Dario
authors.initials: D
authors.lastname: Neri
mesh_terms: D001709:Biotechnology / Q000639:trends; D004797:Enzyme-Linked Immunosorbent Assay; D007700:Kinetics; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D040081:Protein Array Analysis; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D020543:Proteome
chemical_list: D019151:Peptide Library; D010455:Peptides; D011506:Proteins; D020543:Proteome