Cloning and sequence analysis of a mitochondrial gene cluster encoding cytochrome C oxidase subunit III from Trichoderma pseudokoningii.
A mitochondrial gene cluster encoding cytochrome c oxidase subunit III (COX3), an ORF (called ORF250) similar to NADH dehydrogenase subunit VI (ND6), ten tRNA molecules, partial rRNA small subunit and rRNA large subunit from Trichoderma pseudokoningii S38 was cloned and sequenced. These genes are tandemly clustered on the mitochondrial genome of Trichoderma pseudokoningii S38. Phylogenetic analysis showed that cytochrome C oxidase subunits III exhibited high degree of similarity to sequences from Hypocrea jecorina, Verticillium lecanii, Podospora anserine, Neurospora crassa and Magnaporthe grisea (99, 90, 84, 82 and 79% identity, respectively). Prediction of transmembrane helices revealed that COX3 was a transmembrane protein. Northern dot blot analysis showed that the cytochrome c oxidase subunits III gene we had cloned is actively transcribed in the T. pseudokoningii mitochondria.
Wang
Tian-Hong
TH
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong, People's Republic of China. wth@life.sdu.edu.cn
Wu
Zhi-Hong
ZH
Liu
Shi-Li
SL
Huang
Wei
W
D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D029701:Saccharomyces cerevisiae Proteins; D017385:Sequence Homology; D014158:Transcription, Genetic; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics*
D004272:DNA, Mitochondrial; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; C093750:COX3 protein, S cerevisiae; D003576:Electron Transport Complex IV