[Effect of Salvia miltiorrhiza on neuropeptide Y1-36 and calcitonin gene-related peptide in neonatal rats with hypoxia-ischemic brain injury].
OBJECTIVE
To observe the effects of Salvia miltiorrhiza (SM) on levels of neuropeptide Y1-36 and calcitonin gene-related peptide immune reactive substances (ir-NPY, ir-CGRP) in blood plasma and pons-oblongata after hypoxia-ischemic brain injury (HIBI) in neonatal rats.


METHODS
Seven-day old rats were randomized into HIBI group (A), HIBI + SM group (B) and sham operation group(C). And each group was subdivided into 4 subgroups according to the different time after operation. 0.5 ml SM was injected intraperitoneally immediately and every 12 hrs afterwards. Changes of ir-NPY and ir-CGRP levels in plasma and pons-oblongata were observed immediately and 12, 24 and 48 hrs after HIBI by radioimmunoassay.


RESULTS
Plasma levels of ir-NPY and ir-CGRP in different times after HIBI were all significantly raised but those in pons-oblongata were either raised or lowered to a certain degree. Part of the elevated ir-NPY could be reversed by SM injection.


CONCLUSIONS
Central and peripheral neuropeptide Y1-36 and calcitonin gene-related peptide take part in the pathophysiological process of HIBI, SM could partially reverse the abnormal post-HIBI elevation of ir-NPY, which may be one of the pathways of SM in promoting recovery of damaged brain function.
Hong
Xin-ru
XR
Department of Pediatrics, Fuzhou General Hospital, Nanjing Military Area of PLA, Fuzhou 350025.
Wu
Ai-qun
AQ
You
Zhen-dong
ZD
D000818:Animals; D000831:Animals, Newborn; D002545:Brain Ischemia / Q000097:blood*; D015740:Calcitonin Gene-Related Peptide / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D008297:Male; D009478:Neuropeptide Y / Q000097:blood*; D010446:Peptide Fragments / Q000097:blood*; D008517:Phytotherapy; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood*; D027561:Salvia miltiorrhiza / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D009478:Neuropeptide Y; D010446:Peptide Fragments; C090694:neuropeptide Y (1-30); D015740:Calcitonin Gene-Related Peptide