Sodium crocetinate does not alter gut hypercapnic responses or renal energy stores during transient sub-diaphragmatic ischaemia.
OBJECTIVE
To evaluate the protection afforded by trans-sodium crocetinate against dysoxia in an animal model of recurrent sub-diaphragmatic ischaemia.


METHODS
Prospective experimental animal study.


METHODS
University research laboratory


METHODS
Adult male Sprague-Dawley rats.


METHODS
Twelve adult male Sprague-Dawley rats (340-510 g) were anaesthetised with sodium pentobarbitone 60 mg/kg i.p. and ventilated with oxygen and isoflurane via tracheostomy. Six 2-min episodes of sub-diaphragmatic hypotension (mean pressure 30 mmHg) were induced using a sling around the proximal aorta. Before the third and sixth episodes, saline 1.5 ml/kg was injected into the aortic cannula. In six rats, this saline contained trans-sodium crocetinate 50 microg/ml.


RESULTS
Ileal luminal PCO(2) and distal aortic pressure were monitored continuously. Following ischaemic episodes trans-sodium crocetinate had no discernible effect on either degree of PCO(2) elevation or the time to peak PCO(2). No effects on renal energy charge or nucleotide concentrations were detected. UV-visible spectroscopy of the crocetinate preparation showed that some cis isomer was present.


CONCLUSIONS
The findings, although limited to one drug dosage in one animal model, bring into question whether trans-sodium crocetinate affects plasma oxygen diffusivity in vivo. Alternative explanations for the negative findings include a TSC-induced exacerbation of arterio-venous oxygen shunting, the brevity of the dysoxic episodes, and the presence of cis isomer.
Morgan
Thomas J
TJ
Adult Intensive Care, Mater Misericordiae Health Services, Raymond Terrace, 4101, South Brisbane, Queensland, Australia. thomas_morgan@mater.org.au
Venkatesh
Balasubramanian
B
Crerar-Gilbert
Agnieszka
A
Willgoss
Desley
D
Endre
Zoltan H
ZH
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002338:Carotenoids / Q000494:pharmacology*; D004195:Disease Models, Animal; D006935:Hypercapnia / Q000188:drug therapy* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D007511:Ischemia / Q000188:drug therapy* / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D008297:Male; D011446:Prospective Studies; D051381:Rats; D017207:Rats, Sprague-Dawley; D014801:Vitamin A / Q000031:analogs & derivatives
C487773:trans-sodium crocetinate; D014801:Vitamin A; D002245:Carbon Dioxide; D002338:Carotenoids