[The modulation of collagen synthesis and expressions of type I and III collagen mRNAs in aorta smooth muscle cell by PDGF and heparin].
OBJECTIVE
The effect of platelet derived growth factor (PDGF) and heparin on the modulation of human aorta smooth muscle cell(hASMC) proliferation, collagen synthesis, expressions of type I and III collagen mRNAs as well as transforming growth factor-beta (TGF-beta) mRNA were investigated.


METHODS
3H-TdR and 3H-proline incorporation and Northern blot analysis were done. 3H-TdR and 3H-proline incorporation was statistically analyzed with t test among different experimental groups.


RESULTS
In comparison to the control group, PDGF possessed the ability in promoting markedly the DNA synthesis (3H-TdR incorporation: PDGF group vs control, P < 0.01), synthesis and secretion of collagen protein (3H-proline incorporation of SMC and medium: PDGF group vs control, P < 0.01), expressions of type I and type III collagen mRNAs, and transforming growth factor mRNA in hASMC. Whereas heparin significantly decreased the DNA synthesis (heparin group vs control, P < 0.01), synthesis and secretion of collagen protein (heparin group vs control, P < 0.01) of hASMC in vitro. Heparin also inhibited the promoting effect of PDGF on DNA synthesis, the synthesis and secretion of collagen protein, and reduced the up-regulation of expressions of type I and type III collagen mRNAs as well as TGF-beta mRNA that stimulated by PDGF.


CONCLUSIONS
PDGF could promote the collagen synthesis through upregulating type I and type III collagen mRNA expressions while heparin just inhibiting SMC DNA and collagen synthesis effected in the way of anti-atherosclerosis.
Yang
F
F
Cardiovascular Institute, Fu Wai Hospital, CAMS and PUMC, Beijing 100037.
Zhao
P
P
She
M
M
Ye
G
G
Han
X
X
D000925:Anticoagulants / Q000494:pharmacology*; D001013:Aorta, Thoracic / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D024061:Collagen Type III / Q000096:biosynthesis* / Q000235:genetics; D004247:DNA / Q000096:biosynthesis; D005333:Fetus; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011347:Procollagen / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics
D000925:Anticoagulants; D024042:Collagen Type I; D024061:Collagen Type III; D010982:Platelet-Derived Growth Factor; D011347:Procollagen; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D006493:Heparin; D004247:DNA