[The effects of sex hormone on blood vessels].
OBJECTIVE
The protective mechanism of sex hormone on blood vessels was investigated to provide evidences for clinical use of hormone replacement therapy in postmenopausal women.


METHODS
To observe the effects of 17 beta-estradiol (E2), progesterone(P), testosterone (T) and E2 combined with P on the relaxation of umbilical arterial rings (UAR) as well as production of nitric oxide (NO) in cultured umbilical endothelial cells(HUVEC) in vitro.


RESULTS
The results showed that 10(-9)-10(-6) mol/L E2 dilation of UAR, but 10(-5) mol/L E2 showed vague effect. P or T had no relaxation effect, but releasing nitric oxide. When in combined trial P at 10(-5) mol/L inhibited the relaxation effect of E2 at 10(-7)-10(-5) mol/L but didn't inhibit E2 10(-9) mol/L. In HUVEC 10(-9)-10(-5) mol/L E2 stimulated the release of nitric oxide when cells incubated for 10 min and 30 min, but did not influence the NO release at 60 min. P and T have different effects on the release of nitric oxide at different concentrations and incubation time. P also inhibited the NO release induced by E2.


CONCLUSIONS
The results indicated that P actually inhibited the relaxation response and induction of NO release by E2, especially at the high concentration. Therefore, we suggested that to use low doses of E2 combined with low dose P for hormone replacement therapy in prevention and treatment of cardiovascular diseases in postmenopausal patient.
Du
Z
Z
Department of Endocrinology, PUMC Hospital, CAMS and PUMC, Beijing 100730.
Sun
M
M
Wang
B
B
Qi
Y
Y
Ge
Q
Q
D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D006801:Humans; D009126:Muscle Relaxation / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009569:Nitric Oxide / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D013739:Testosterone / Q000494:pharmacology; D014469:Umbilical Arteries / Q000166:cytology / Q000187:drug effects
D012739:Gonadal Steroid Hormones; D009569:Nitric Oxide; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol