[Effect of bushen yizhi recipe on neurotransmitter release in A beta segment neurotoxin induced NG108-15 cellular model of Alzheimer disease].
OBJECTIVE
To observe the effect of Bushen Yizhi Recipe (BSYZR) on neurotransmitter release in A beta segment neurotoxin induced NG108-15 cellular model of Alzheimer disease (AD).


METHODS
The levels of choline acetyltransferase (ChAT) activity, synapsin and functional synapse formation rate in the cellular model treated with BSYZR containing serum were determined by Western blot analysis, immunoradiometric assay and electrophysiologic technique.


RESULTS
BSYZR containing serum treatment could cause increase of ChAT activity and synapsin level in model cells, as compared with those in normal control model cells treated with non-drug containing serum, it also could regulate the release capacity of transmitter and raise the functional synapse formation.


CONCLUSIONS
BSYZR could reduce the reaction of cell to A beta neurotoxin, indicating that it could be antagonistic to the pathological development of AD by means of raising the neurotransmitter release capacity.
Zhong
Zhen-guo
ZG
Institute of Neuroscience, 2nd Affiliated Hospital of Guangxi College of TCM, Nanning 530001.
Liu
Mao-cai
MC
Lai
Shi-long
SL
D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D009498:Neurotoxins / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D016704:Synapsins / Q000378:metabolism
D016229:Amyloid beta-Peptides; D004365:Drugs, Chinese Herbal; D009498:Neurotoxins; D018377:Neurotransmitter Agents; D016704:Synapsins; D002795:Choline O-Acetyltransferase