Inhibitory effects of formoterol on lipopolysaccharide-induced premature delivery through modulation of proinflammatory cytokine production in mice.
Previous studies have demonstrated that formoterol, a beta2-adrenoceptor agonist, has potent tocolytic effects in rats. The aim of the present study was to determine whether formoterol treatment affects proinflammatory cytokine production in a lipopolysaccharide (LPS)-treated premature delivery mouse model. Formoterol was continuously administered by osmotic pump and the number of fetuses in the uteri were counted. Samples of amniotic fluid and plasma were collected 8 and 16 h after systemic administration of LPS. LPS induced premature delivery and an increase in prostaglandin F(2alpha) (PGF(2alpha)), interleukin 1alpha (IL-1alpha), IL-6 and IL-10 in the amniotic fluid, and an increase in IL-6 in plasma. Formoterol blocked all changes except the increase in IL-10. These data indicate that formoterol exerts inhibitory effects on proinflammatory cytokine production, and these effects may play an important role in the prevention of premature delivery.
Shinkai
N
N
Research Department, Saitama Daiichi Pharmaceutical Co Ltd, 8-1 Minamisakae-cho Kasukabe, Saitama 344-0067, Japan. shinkai@sdsk.co.jp
Takasuna
K
K
Takayama
S
S
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis*; D003864:Depression, Chemical; D015237:Dinoprost / Q000097:blood; D004983:Ethanolamines / Q000494:pharmacology*; D005260:Female; D000068759:Formoterol Fumarate; D015918:Infusion Pumps, Implantable; D007375:Interleukin-1 / Q000096:biosynthesis / Q000097:blood; D016753:Interleukin-10 / Q000096:biosynthesis / Q000097:blood; D015850:Interleukin-6 / Q000096:biosynthesis / Q000097:blood; D008070:Lipopolysaccharides; D008098:Litter Size / Q000187:drug effects; D051379:Mice; D008809:Mice, Inbred C3H; D023421:Models, Animal; D007752:Obstetric Labor, Premature / Q000276:immunology*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology*
D000318:Adrenergic beta-Agonists; D016207:Cytokines; D004983:Ethanolamines; D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016753:Interleukin-10; D015237:Dinoprost; D000068759:Formoterol Fumarate