Distribution of adenoviral vector in brain after intravenous administration.
The delivery of transgenes to the central nervous system (CNS) can be a valuable tool to treat CNS diseases. Various systems for the delivery to the CNS have been developed; vascular delivery of viral vectors being most recent. Here, we investigated gene transfer to the CNS by intravenous injection of recombinant adenoviral vectors, containing green fluorescence protein (GFP) as a reporter gene. Expression of GFP was first observed 6 days after the gene transfer, peaked at 14 days, and almost diminished after 28 days. The observed expression of GFP in the CNS was highly localized to hippocampal CA regions of cerebral neocortex, inferior colliculus of midbrain, and granular cell and Purkinje cell layers of cerebellum. It is concluded that intravenous delivery of adenoviral vectors can be used for gene delivery to the CNS, and hence the technique could be beneficial to gene therapy.
Moon
Chanil
C
Division of Cardiology, Department of Internal Medicine, School of Medicine, Eulji University, Seoul, Korea.
Moon
Cheil
C
Kang
Wha-Sun
WS
Jeong
Dae-Chul
DC
Jin
Jong-Youl
JY
D000260:Adenoviruses, Human / Q000302:isolation & purification*; D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000821:virology*; D002531:Cerebellum / Q000166:cytology / Q000821:virology; D005260:Female; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000008:administration & dosage / Q000302:isolation & purification / Q000493:pharmacokinetics*; D049452:Green Fluorescent Proteins; D006624:Hippocampus / Q000821:virology; D007245:Inferior Colliculi / Q000821:virology; D007275:Injections, Intravenous; D008164:Luminescent Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009457:Neuroglia / Q000821:virology; D009474:Neurons / Q000821:virology*; D011689:Purkinje Cells / Q000821:virology; D017966:Pyramidal Cells / Q000821:virology; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D013623:Tail / Q000098:blood supply; D014018:Tissue Distribution
D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins