[Aminoglycoside ototoxicity associated with mitochondrial DNA mutation].
OBJECTIVE
To confirm the effect of genetic susceptibility to aminoglycoside ototoxicity.


METHODS
Mitochondrial DNA (mtDNA) from 62 members of 9 aminoglycoside induced deafness families was analysed by PCR-Restriction endonuclease digestion.


RESULTS
The nucleotide 1555A-->G mutation in 12S rRNA gene of mtDNA was identified in 20 members of 5 families.


CONCLUSIONS
These findings indicate that this mutation is a molecular basis for genetic susceptibility to the ototoxic effect of aminoglycosides. The relationship between the audiovestibular changes and genetic susceptibility to aminoglycoside ototoxicity and the pathogenetic mechanism of susceptible deafness are discussed. The possibility that other gene mutations can also predispose to aminoglycoside ototoxicity is proposed.
Ke
X
X
Department of Otolaryngology, First Hospital, Beijing Medical University, Beijing 100034.
Qi
Y
Y
Gu
Z
Z
Zhang
Z
Z
Zhang
W
W
Jiang
S
S
Liu
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D004272:DNA, Mitochondrial / Q000235:genetics*; D003638:Deafness / Q000139:chemically induced / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010375:Pedigree
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004272:DNA, Mitochondrial