The wbiA locus is required for the 2-O-acetylation of lipopolysaccharides expressed by Burkholderia pseudomallei and Burkholderia thailandensis.
Burkholderia pseudomallei and Burkholderia thailandensis express similar O-antigens (O-PS II) in which their 6-deoxy-alpha-L-talopyranosyl (L-6dTalp) residues are variably substituted with O-acetyl groups at the O-2 or O-4 positions. In previous studies we demonstrated that the protective monoclonal antibody, Pp-PS-W, reacted with O-PS II expressed by wild-type B. pseudomallei strains but not by a B. pseudomallei wbiA null mutant. In the present study we demonstrate that WbiA activity is required for the acetylation of the L-6dTalp residues at the O-2 position and that structural modification of O-PS II molecules at this site is critical for recognition by Pp-PS-W.
Brett
Paul J
PJ
Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, 3330 Hospital Drive NW, T2N 4N1, Calgary, AB, Canada.
Burtnick
Mary N
MN
Woods
Donald E
DE
D000107:Acetylation; D000595:Amino Acid Sequence; D019117:Burkholderia / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016957:Burkholderia pseudomallei / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D000939:Epitopes / Q000276:immunology; D005798:Genes, Bacterial; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism; D019901:Locus Control Region; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D016415:Sequence Alignment
D004269:DNA, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides; D019081:O Antigens