[Establishment of hybridomas secreting monoclonal antibodies to HIV-gp41 and HCV-NS3].
OBJECTIVE
To establish the monoclonal antibodies of HIV and HCV for developing serological diagnostic method.


METHODS
The antigens of HIV gp41 and HCV NS3 expressed from bacteria were mixed to immunize BALB/c mice. The hybridoma cell lines were obtained by fusing SP2/0 myeloma cells with spleen cells from immunized mice.


RESULTS
Four hybridoma cell lines secreting specific antibodies against gp41 of HIV and six hybridoma cell lines secreting antibodies to NS3 of HCV were obtained. ELISA sandwich method for detection of viral antigens with McAbs was developed.


CONCLUSIONS
A quick and convenient method for obtaining McAbs was developed using mixture of different antigens for immunizition of mice. The monoclonal antibodies had high senusitivity and were stably secreted by hybridoma cell lines. This technique is worthy to be popularized.
Zhang
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S
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052.
Pi
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G
Gu
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D015700:HIV Envelope Protein gp41 / Q000235:genetics* / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000276:immunology; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015700:HIV Envelope Protein gp41; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases