[Effect of 15-methyl PGF2 alpha methyl ester combined with tyrosine hydrazide on early pregnancy in mice].
OBJECTIVE
To observe the effect of 15-methyl PGF2 alpha methyl ester (PG05) in combination with tyrosine hydrazide (TH) on early pregnancy in mice.


METHODS
The pregnancy rate of mice treated with PG05 and TH was counted and serum progesterone level was measured by RIA.


RESULTS
Administration of TH at 90 mg.kg.day-1 to mice on day 7-9 of gestation enhanced significantly the termination of early pregnancy induced by PG05 at 0.2 mg-1.kg-1 for 2 days. Serum progesterone level of mice treated with PG05 combined with TH was found to be markedly lower than that of animals given PG05 or TH alone. TH at 30 micrograms-1.ml-1 inhibited significantly the activity of 3 beta-HSD of luteal cells in rat. However, PG05 at 10 micrograms-1.ml-1 had no influence upon inhibitory effect of 3 beta-HSD activity induced by TH. TH at 150 mg-1.kg-1 for 3 days did not affect the progesterone receptor level of utering cytoplasma in pseudopregnant rats.


CONCLUSIONS
The inhibitory effect of TH on the production of progesterone may be the main mechanism to terminate early pregnancy when used synergically with PG05.
Zheng
W
W
Department 2 of Pharmacology, Institute of Materia Medica, CAMS and PUMC, Beijing 100050.
Duan
H
H
Wang
N
N
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000019:Abortifacient Agents / Q000494:pharmacology*; D000818:Animals; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004357:Drug Synergism; D005260:Female; D008184:Luteal Cells / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000097:blood / Q000187:drug effects*; D011374:Progesterone / Q000097:blood; D011555:Pseudopregnancy / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011980:Receptors, Progesterone / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000494:pharmacology*
D000019:Abortifacient Agents; D011980:Receptors, Progesterone; D014443:Tyrosine; D011374:Progesterone; C027809:3,5-diiodo-L-tyrosine hydrazide; C030319:prostaglandin F2alpha 15-methyl ether; D015237:Dinoprost; D015096:3-Hydroxysteroid Dehydrogenases