title: [Typing of HLA-AB by Reverse PCR-SSOP and Clinical Application]
abstract: A rapid and accurate method of DNA typing for HLA was established to compensate the unsatisfactory serological typing for HLA before transplantation. DNA typing for HLA using by reverse polymerase chain reaction with sequence-spcific oligo probe (reverse PCR-SSOP) could detect HLA-A(*0101 - 8001) and B(*07021 - 8201). The results showed that HLA-AB alleles were successfully analysed in 60 matching subjects and 16 control DNAs from UCLA by reverse PCR-SSOP without false negtive and false positive results. The results were concordance with those of UCLA. The error rate of serological typing was 6.4% for HLA-A and 7.4% for HLA-B. The serological typing missed HLA-A24 and HLA-B46 for two patients with leukemia respectively. Our results suggest that DNA typing for HLA by reverse PCR-SSOP has proved to be a high-resolution, high-specificity, rapid and accurate technique, suitable for clinical application with a greater precision than serological typing.
authors.affiliation: Department of Immunogenetics, Shanghai Blood Center, Shanghai 200051, China.
authors.forename: Ming-Liang
authors.initials: ML
authors.lastname: Feng
authors.forename: Yun
authors.initials: Y
authors.lastname: Ji
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.forename: Qiong
authors.initials: Q
authors.lastname: Lu
authors.forename: Yue-Hua
authors.initials: YH
authors.lastname: Ji
authors.forename: Gong-Liang
authors.initials: GL
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang