Molecular basis of the effect of potassium on heterologously expressed pacemaker (HCN) channels.
Hyperpolarization-activated cyclic-nucleotide-gated (HCN) channels modulate the firing rates of neuronal and cardiac pacemaker cells. HCN channels resemble voltage-gated K+ channels structurally, but much less is known about their structure-function correlation. Although modulation of K+ channel gating by external K+ is a well-known phenomenon, such a link has not been established for HCN channels. Here we examined the effects of external permeant (K+, Na+ and Li+) and non-permeant (NMG+) ions on HCN1 and HCN2 gating. Substituting 64 of 96 mM external K+ with Na+, Li+ or NMG+ positively shifted steady-state activation (approximately 13 mV), and preferentially slowed activation of HCN1. Mutating the pore variant C-terminal to the GYG motif in HCN1, A352, to the analogous conserved Asp in K+ channels or Arg in HCN2 produced a significant hyperpolarizing activation shift (by 5-15 mV), slowed gating kinetics (up to 6-fold), and abolished or attenuated gating responses to external K+. Whereas Na+, Li+ and NMG+ substitutions produced depolarizing activation shifts of HCN2 similar to those of HCN1, deactivation but not activation of HCN2 was exclusively decelerated. We conclude that gating and permeation of HCN channels are coupled, and that modulation of this 'pore-to-gate' coupling by external K+ is isoform-specific.
Azene
Ezana M
EM
Institute of Molecular Cardiobiology, The Johns Hopkins University School of Medicine, 720 Rutland Avenue/Ross 844, Baltimore, MD 21205, USA.
Xue
Tian
T
Li
Ronald A
RA
D020816:Amino Acid Motifs; D000818:Animals; D001120:Arginine; D001224:Aspartic Acid; D016384:Consensus Sequence; D054815:Cyclic Nucleotide-Gated Cation Channels; D005998:Glycine; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D015640:Ion Channel Gating; D007473:Ion Channels / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008094:Lithium / Q000494:pharmacology; D008536:Meglumine / Q000494:pharmacology; D009124:Muscle Proteins / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009865:Oocytes; D011188:Potassium / Q000494:pharmacology*; D015221:Potassium Channels; D012964:Sodium / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014443:Tyrosine; D014981:Xenopus
D054815:Cyclic Nucleotide-Gated Cation Channels; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels; D009124:Muscle Proteins; D015221:Potassium Channels; D001224:Aspartic Acid; D014443:Tyrosine; D008536:Meglumine; D001120:Arginine; D008094:Lithium; D012964:Sodium; D011188:Potassium; D005998:Glycine