title: Direct identification of Staphylococcus aureus from positive blood culture bottles.
abstract: Fluorescence in situ hybridization (FISH) using peptide nucleic acid (PNA) probes targeting Staphylococcus aureus 16S rRNA is a novel method for direct identification of S. aureus from positive blood culture bottles. The test (S. aureus PNA FISH) is performed on smears made directly from positive blood culture bottles with gram-positive cocci in clusters (GPCC) and provides results within 2.5 h. A blinded comparison of S. aureus PNA FISH with standard identification methods was performed in collaboration with eight clinical microbiology laboratories. A total of 564 routine blood culture bottles positive for GPCC recovered from both aerobic and anaerobic media from three different manufacturers (ESP, BACTEC, and BacT/Alert) were included in the study. The sensitivity and specificity of S. aureus PNA FISH were 100% (57 of 57) and 99.2% (116 of 117), respectively, with 174 GPCC-positive ESP blood culture bottles, 98.5% (67 of 68) and 98.5% (129 of 131), respectively, with 200 GPCC-positive BACTEC blood culture bottles, and 100% (74 of 74) and 99.1% (115 of 116), respectively, with 190 GPCC-positive BacT/Alert blood culture bottles. It is concluded that S. aureus PNA FISH performs well with commonly used continuously monitoring blood culture systems.
authors.affiliation: Applied Biosystems, Bedford, Boston , Massachusetts, USA.
authors.forename: Kenneth
authors.initials: K
authors.lastname: Oliveira
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Brecher
authors.forename: Annette
authors.initials: A
authors.lastname: Durbin
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Shapiro
authors.forename: Donald R
authors.initials: DR
authors.lastname: Schwartz
authors.forename: Paola C
authors.initials: PC
authors.lastname: De Girolami
authors.forename: Joanna
authors.initials: J
authors.lastname: Dakos
authors.forename: Gary W
authors.initials: GW
authors.lastname: Procop
authors.forename: Deborah
authors.initials: D
authors.lastname: Wilson
authors.forename: Chad S
authors.initials: CS
authors.lastname: Hanna
authors.forename: Gerhard
authors.initials: G
authors.lastname: Haase
authors.forename: Heidrun
authors.initials: H
authors.lastname: Peltroche-Llacsahuanga
authors.forename: Kimberle C
authors.initials: KC
authors.lastname: Chapin
authors.forename: Michael C
authors.initials: MC
authors.lastname: Musgnug
authors.forename: Michael H
authors.initials: MH
authors.lastname: Levi
authors.forename: Cynthia
authors.initials: C
authors.lastname: Shoemaker
authors.forename: Henrik
authors.initials: H
authors.lastname: Stender
mesh_terms: D001431:Bacteriological Techniques; D003470:Culture Media; D015342:DNA Probes; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D020135:Peptide Nucleic Acids; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D003470:Culture Media; D015342:DNA Probes; D020135:Peptide Nucleic Acids