[Humoral immune response in mice to hybrid nucleic acid vaccines containing Plasmodium falciparum merozoite surface protein 1 block 17-based gene].
OBJECTIVE
To analyse the humoral immune response in mice to nucleic acid vaccines (VR1012/HG-MSP1-17 for intracellular expression or VR1012/TPA/HG-MSP-17 for secretion) containing Plasmodium falciparum merozoite surface protein 1 (MSP1) 17 block gene and gene fragment of several T cell epitopes from MSA1, MSA2, RESA, IL-1 and TT.


METHODS
BALB/c or C57BL/6 mice received three times intramuscular immunization with 200 micrograms/100 microliters or 100 micrograms/100 microliters of VR1012/HG-MSP1-17 or VR1012/TPA/HG-MSP1-17 per mouse each time. Anti-HG or anti-MSP1-17 antibodies were monitored by indirect ELISA.


RESULTS
BALB/c and C57BL/6 mice immunized with 100 micrograms/100 microliters of VR1012/HG-MSP1-17 per mouse raised significantly anti-HG and anti-MSP1-17 antibodies, but the levels of antibodies were not high. BALB/c mice immunized with 200 micrograms/100 microliters of VR1012/HG-MSP1-17 per mouse raised higher anti-HG antibodies but not anti-MSP1-17 antibodies. BALB/c mice immunized with 200 micrograms/100 microliters of VR1012/TPA/HG-MSP1-17 per mouse raised low level of anti-HG antibodies only.


CONCLUSIONS
VR1012/HG-MSP1-17 is more immunogenic than VR1012/TPA/HG-MSP1-17.
Miao
J
J
Department of Parasitology, Fourth Military Medical University, Xi'an 710032.
Li
X
X
Xue
C
C
Zhen
R
R
Liu
Z
Z
Qin
E
E
Yu
Q
Q
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D000953:Antigens, Protozoan / Q000276:immunology / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D017780:Malaria Vaccines / Q000276:immunology / Q000494:pharmacology*; D020066:Merozoite Surface Protein 1 / Q000276:immunology / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010963:Plasmodium falciparum / Q000737:chemistry / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology / Q000494:pharmacology*; D019444:Vaccines, DNA / Q000276:immunology / Q000494:pharmacology
D000953:Antigens, Protozoan; D017780:Malaria Vaccines; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins; D019444:Vaccines, DNA