Anti-oxidant activities of the extracts from the herbs of Artemisia apiacea.
The anti-oxidant activities of the various fractions from the herbs of Artemisia apiacea were investigated. The n-hexane and n-butanol fractions were found to cause significant free radical scavenging effects on DPPH, their scavenging potencies as indicated in IC(50) values, being 230.1 and 183.7 microg/ml, respectively. The n-butanol fraction exhibited a significant decrease in serum transaminase activities elevated by hepatic damage induced by CCl(4)-intoxication in rats. All fractions tested exhibited a lipid peroxidation causing a significant decrease in MDA production in TBA-reactant assay. The n-butanol fraction was the strongest in the increase in the anti-oxidant enzymes such as hepatic cytosolic superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH-px) activities in CCl(4)-intoxicated rats. These results suggest that the herbs of A. apiacea possess not only the anti-oxidant, but also the activities in CCl(4)-intoxicated rats. Especially, the n-butanol extract was found to cause significant increases in the rat liver cytosolic SOD, catalase, GSH-px activities as well as a significant decrease in the MDA production.
Kim
Kyoung Soon
KS
College of Pharmacy, Seoul National University, San 56-1, Shillim-Dong, Gwanak-Gu, South Korea.
Lee
Sanghyun
S
Lee
Yeon Sil
YS
Jung
Sang Hoon
SH
Park
Youmie
Y
Shin
Kuk Hyun
KH
Kim
Bak-Kwang
BK
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D019306:Artemisia; D001219:Aspartate Aminotransferases / Q000097:blood; D001713:Biphenyl Compounds; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy / Q000378:metabolism; D016166:Free Radical Scavengers / Q000737:chemistry; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D012997:Solvents
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; D008315:Malondialdehyde; C004931:1,1-diphenyl-2-picrylhydrazyl; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase