Hyperlipidemia coincides with reversible growth impairment of cultured human autologous endothelial cells.
Patient-related risk factors for the growth of autologous endothelial cells were assessed in a clinical series of 100 consecutive recipients of in vitro endothelialized prosthetic vascular grafts. For all patients, the indication for bypass operation was arteriosclerotic occlusive disease of the distal arteries. Endothelial cells were harvested from a small piece of subdermal vein and cultured in medium containing 20% of autologous serum. Growth was continually monitored. In cultures that failed to grow, the autologous serum supplement to the culture medium was replaced by pooled homologous serum from young healthy donors. The comparison of a multitude of serum parameters between patients whose endothelial cells failed to grow and those showing normal growth revealed a significant difference in serum lipid content: triglycerides: 4.76 +/- 3.36 versus 2.83 +/- 2.28 mmol/L (p = .001); cholesterol: 6.78 +/- 1.69 versus 5.69 +/- 1.32 mmol/L (p = .003); and lipoprotein (a): 35.9 +/- 28.3 versus 22.2 +/- 26.6 mg/dl (p = .04). Following serum exchange with low-lipid pool serum that contained 1.74 mmol/L triglycerides, 4.86 mmol/L cholesterol, 5 mg/dl lipoprotein (a), and 5.79 mmol/L glucose, a remarkable recovery occurred in 85% of these cultures, resulting in fully restored proliferative capacity. As a consequence, population doubling time did not differ between the two groups at any point in time and mass cultures sufficient for confluent graft endothelialization were obtained with hardly any delay. The authors conclude that hyperlipidemia may lead to growth impairment of cultured human endothelial cells. This growth inhibition is reversible if the supplemented autologous serum is replaced by pooled serum with low lipid content.
Meinhart
Johann
J
First Department of Surgery and Ludwig Boltzmann Institut for Applied Cardiovascular Biology, City Hospital Vienna-Lainz, Vienna, Austria. mej@1ch.kh1.magwien.gv.at
Halbmayer
Walter-Michael
WM
Deutsch
Manfred
M
Zilla
Peter
P
D000368:Aged; D002455:Cell Division / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002784:Cholesterol / Q000097:blood*; D003470:Culture Media; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000378:metabolism* / Q000503:physiopathology; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D020127:Recovery of Function / Q000502:physiology; D012016:Reference Values; D014280:Triglycerides / Q000097:blood*; D014680:Veins / Q000166:cytology / Q000254:growth & development / Q000378:metabolism
D003470:Culture Media; D008074:Lipoproteins; D014280:Triglycerides; D002784:Cholesterol