[The effect of transforming growth factor-beta 1 on the secretion of collagen type IV in cultured human trabecular cells].
OBJECTIVE
To investigate the effect of transforming growth factor-beta 1 (TGF-beta 1) on the expression of collagen type IV in cultured human trabecular cells.


METHODS
On the basis of successful culture of human trabecular cells, we detected the content of collagen type IV in the media and covers of trabecular cells, treated with TGF-beta 1 (1 ng/ml), by using enzyme-linked immunosorbent assay(ELISA) and immunohistochemical techniques respectively.


RESULTS
ELISA method demonstrated that, in the first four days, there was statiscally significant difference between TGF-beta 1 treated and control groups with the collagen type IV content of 187.50 +/- 29.01 pg/ml and 93.75 +/- 20.50 pg/ml respectively, then in the second four days, there was no statistically significant difference between experimental group of 128.75 +/- 52.02 pg/ml and control group of 91.25 +/- 21.36 pg/ml. Neither statistical difference was found between two treated groups, nor was between two control groups. Immunohistochemical techniques showed, the positive staining of collagen type IV was stronger in cell covers of experimental group treated with TGF-beta 1 for 12 days than that of control group.


CONCLUSIONS
TGF-beta 1 can increase the expression of collagen type IV in cultured trabecular cells, it may be among causes of abnormal accumulation of extracellular matrices in trabecular meshwork of eyes with primary open angle glaucoma.
Li
M
M
Department of Ophthalmology, First Clinic Medical College, Peking University, Beijing 100034, China.
Li
M
M
D002478:Cells, Cultured; D024141:Collagen Type IV / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D014129:Trabecular Meshwork / Q000166:cytology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1
D024141:Collagen Type IV; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1