[Anti-gastric ulcer sesquiterpene lactone glycosides from Crepis napifera].
OBJECTIVE
The anti-gastric ulcer constituents from the roots of Crepis napifera (Franch) Babc (Compositae) were studied.


METHODS
Solvent partition, Si gel and Rp-18 column chromatography, crystallization and spectral methods were used to extract, isolate and identify two compounds. The activity of compound 1 was tested on the rat stomach by determining the effect on aspirin-induced gastric lesions and on histamine-stimulated gastric acid secretion.


RESULTS
Two sesquiterpene lactone glycosides, taraxinic acid-1'-O-beta-D-glucopyranoside (1) and 11,13-dihydro-taraxinic acid-1'-O-beta-D-glucopyranoside (2) were obtained. Compound 1 at the dose of 80 mg.kg-1 p.o. inhibited significantly the development of aspirin-induced gastric lesions in the rat and at an i.v. dose of 70 mg.kg-1 did not affect histamine-stimulated gastric acid secretion in the lumen-perfused rat stomach.


CONCLUSIONS
Compound 1 is the active component of the plant which protects gastric mucosa and exhibits anti-gastric ulcer action.
Wu
Shao-hua
SH
Laboratory of Phytochemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China.
Luo
Xiao-dong
XD
Ma
Yun-bao
YB
Hao
Xiao-jiang
XJ
Wu
Da-gang
DG
D000818:Animals; D000897:Anti-Ulcer Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D001241:Aspirin; D027468:Crepis / Q000737:chemistry*; D004195:Disease Models, Animal; D005260:Female; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D008297:Male; D008968:Molecular Conformation; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy*
D000897:Anti-Ulcer Agents; D012717:Sesquiterpenes; C034545:taraxinic acid 1'-O-beta-glucopyranoside; D001241:Aspirin