[Analysis on karyotypes of ANKA strain of Plasmodium berghei].
OBJECTIVE
To analyze the molecular karyotypes of ANKA strain of Plasmodium berghei and demonstrate the size and number of chromosomes.


METHODS
To isolate the genome DNA of P. berghei ANKA strain and analyze molecular karyotypes through CHEF-III pulsed field gel electrophoresis (PFGE).


RESULTS
The number of chromosomes was found to be 14, and their size ranged from 0.6 Mb to 3 Mb. Chromosomes number 5 to 7 and 9 to 12 appeared co-migrated in the gel.


CONCLUSIONS
PFGE technique is useful for analyzing the molecular karyotypes and may be also useful for further study to locate the special gene on chromosomes and carry out the genetic characters and mechanism of drug resistance.
Chen
Y D
YD
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Zhang
J X
JX
Ling
B Y
BY
D000818:Animals; D004351:Drug Resistance; D016521:Electrophoresis, Gel, Pulsed-Field; D007621:Karyotyping; D051379:Mice; D010962:Plasmodium berghei / Q000145:classification / Q000235:genetics*