title: Methods for measuring the infrared spectra of biological cells.
abstract: Infrared (IR) spectroscopy of biological cells is a growing area of research, with many papers focusing on differences between the spectra of cancerous and noncancerous cells. Much of this research has been performed using a monolayer of dehydrated cells. We posit that the use of monolayers can introduce artefacts that lead to an apparent but inaccurate measurement of differences between cancerous and noncancerous cells. Additionally, the use of dried cells complicates the extraction of biochemical information from the IR spectra. We demonstrate that using suspensions of viable cells in aqueous suspension reduces measurement artefacts and facilitates determining the concentration of the major biochemical components via a linear least-squares fit of the component spectra to the spectrum of the cells.
authors.affiliation: Bioscience Division. MS E535, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
authors.forename: Judith R
authors.initials: JR
authors.lastname: Mourant
authors.forename: Rowena R
authors.initials: RR
authors.lastname: Gibson
authors.forename: Tamara M
authors.initials: TM
authors.lastname: Johnson
authors.forename: Susan
authors.initials: S
authors.lastname: Carpenter
authors.forename: Kurt W
authors.initials: KW
authors.lastname: Short
authors.forename: Yujiro R
authors.initials: YR
authors.lastname: Yamada
authors.forename: James P
authors.initials: JP
authors.lastname: Freyer
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000737:chemistry; D002460:Cell Line; D004247:DNA / Q000737:chemistry; D003890:Desiccation; D005347:Fibroblasts / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D008297:Male; D009375:Neoplasms, Glandular and Epithelial / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry; D012313:RNA / Q000737:chemistry; D051381:Rats; D011916:Rats, Inbred F344; D012680:Sensitivity and Specificity; D013055:Spectrophotometry, Infrared / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014407:Tumor Cells, Cultured / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D008055:Lipids; D011506:Proteins; D014867:Water; D012313:RNA; D004247:DNA