[Screening and cloning of genes encoding Schistosoma japonicum antigens related to the serum antibodies in Mirotus fortis].
OBJECTIVE
To understand and identify the molecules related to the natural resistance to Schistosoma japonicum infection in Mirotus fortis.


METHODS
Sera from Mirotus fortis without schistosome infection were collected. The S. japonicum adult worm cDNA library was immunologically screened with the sera. The positive recombinants were identified, cloned, sequenced and analysed with software and internet.


RESULTS
Seven genes encoding antigens relevant to sera antibodies in Mirotus fortis were cloned and sequenced. These antigens included glyceraldehyde 3-phosphate dehydrogenase (GAPDH), serine protease inhibitors (SERPIN), 70 kDa heat shock protein (HSP70), 22.6 kDa membrane-associated antigen, paramyosin (Sj97), cytochrome C and cathepsin B.


CONCLUSIONS
Many protein molecules might have been involved in natural resistance to S. japonicum infection in Mirotus fortis. The above 7 kinds of molecules may be identified as new candidates of vaccine against S. japonicum infection.
Yan
Y T
YT
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai, 200025.
Liu
S X
SX
Song
G C
GC
Xu
Y X
YX
He
Y K
YK
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000235:genetics*; D003411:Arvicolinae / Q000276:immunology*; D003001:Cloning, Molecular; D018509:DNA, Helminth / Q000235:genetics; D015723:Gene Library; D012549:Schistosoma japonicum / Q000235:genetics* / Q000276:immunology; D012554:Schistosomiasis japonica / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018509:DNA, Helminth