title: Determination of 8-hydroxy-2'-deoxyguanosine in untreated urine by capillary electrophoresis with UV detection.
abstract: A capillary electrophoresis method with UV detection was developed for the determination of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in untreated urine samples. The calibration graph for 8-OHdG in urine is linear in the concentration range 10-500 mg/l. and the detection limit is 5 mg/l (17 microM). 8-OHdG was determined in urine from oncological patients treated by radiation therapy. Its concentrations relative to creatinine were found to be in the range 10-47 microg 8-OHdG/l mg creatinine (4-19 micromol 8-OHdG/mmol creatinine). The overall time of the analysis of a urine sample was less than 15 min.
authors.affiliation: 3rd Faculty of Medicine, Centre of Biomedical Sciences, Charles University, Ruská 87, 100 00 Prague 10, Czech Republic.
authors.forename: Vladimira
authors.initials: V
authors.lastname: Kvasnicová
authors.forename: Eva
authors.initials: E
authors.lastname: Samcová
authors.forename: Alice
authors.initials: A
authors.lastname: Jursová
authors.forename: Ivan
authors.initials: I
authors.lastname: Jelínek
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D002138:Calibration; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000652:urine; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine