Complex regulation of the Bacillus subtilis aconitase gene.
The roles of the CcpC, CodY, and AbrB proteins in regulation of the Bacillus subtilis aconitase (citB) gene were found to be distinct and to vary with the conditions and phase of growth. CcpC, a citrate-inhibited repressor that is the primary factor regulating citB expression in minimal-glucose-glutamine medium, also contributed to repression of citB during exponential-phase growth in broth medium. A null mutation in codY had no effect on citB expression during growth in minimal medium even when combined with ccpC and abrB mutations. However, a codY mutation slightly relieved repression during exponential growth in broth medium and completely derepressed citB expression when combined with a ccpC mutation. An abrB mutation led to decreased expression of citB during stationary phase in both broth and minimal medium. All three proteins bound in vitro to specific and partially overlapping sites within the citB regulatory region. Interaction of CcpC and CodY with the citB promoter region was partially competitive.
Kim
Hyun-Jin
HJ
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Kim
Sam-In
SI
Ratnayake-Lecamwasam
Manoja
M
Tachikawa
Kiyoshi
K
Sonenshein
Abraham L
AL
Strauch
Mark
M
D000154:Aconitate Hydratase / Q000235:genetics* / Q000378:metabolism*; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division / Q000235:genetics; D002951:Citrates / Q000378:metabolism; D003470:Culture Media; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
C060762:AbrB protein, Bacillus subtilis; D001426:Bacterial Proteins; C107075:CitB protein, Klebsiella pneumoniae; D002951:Citrates; D003470:Culture Media; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C069286:catabolite control proteins, bacteria; D000154:Aconitate Hydratase