[Application of IL-1 beta and 8 mRNA and some other indices in monitoring the activity of ulcerative colitis].
OBJECTIVE
To evaluate the monitoring action of the expression of IL-1 beta, IL-8 mRNA, myeloperoxidase (MPO) and superoxide dismutase (SOD) activity in the active ulcerative celitis (UC).


METHODS
Twenty active UC patients, 23 inactive UC patients; and 14 non-UC inflammation patients were selected. Twelve patients who complained of flatulence, abdominal pain and constipation receiving endoscopic examination were used as control. MPO and SOD activity, IL-1 beta and IL-8 mRNA expression (hybridization in situ) were determined on the endoscopic biopsy specimens of all patients. In 20 active UC patients, 14 patients received endoscopic examination and 4 indices detection were done once again after 2 months' treatment with prednisone and SASP.


RESULTS
Mucosal MPO activities of active UC patients, inactive UC patients, and non-UC inflammation patients are higher than those of control patients, (19.37 +/- 0.54, 11.59 +/- 1.41, and 12.97 +/- 0.49) U/g tissue vs (9.49 +/- 0.51) U/g tissue (P < 0.01). SOD activities are lower than that of control patients, (5.03 +/- 07,7. 7.66 +/- 0.79, and 6.98 +/- 0.61) U/mg protein vs (8.82 +/- 0.58) U/mg protein (P < 0.05). Mucosal MPO activity of active UC patients is also higher than that of inactive and non-UC inflammation patients (P < 0.01); while SOD activity is lower than them (P < 0.01). After 2 months' medical treatment, MPO activity of 14 active UC patients decreased, (12.61 +/- 0.74) U/g tissue vs (19.31 +/- 0.44) U/g tissue (P < 0.01), while SOD activity elevated (7.44 +/- 0.55) U/mg protein vs (5.10 +/- 1.05) U/mg protein (P < 0.05), compared with that of before treatment. Positive expression of IL-1 beta mRNA appeared in the epithelial and inflammatory cells of all active UC patients, 9 inactive UC patients, and 7 non-UC inflammation patients. While expression of IL-8 mRNA only appeared positively in all active UC patients. In 14 active UC patients, there were no detection of interleukin mRNA expression after 2 months' treatment.


CONCLUSIONS
Mucosal MPO, SOD, IL-1 beta, and IL-8 mRNA could be used as 4 indices monitoring the activity of UC. And IL-1 beta mRNA is also helpful to supervise early or late UC activity.
Zhu
F
F
Department of Gastroenterology, PUMC Hospital, CAMS and PUMC, Beijing 100730.
Qian
J
J
Pan
G
G
D015415:Biomarkers; D003093:Colitis, Ulcerative / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D009195:Peroxidase / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D013482:Superoxide Dismutase / Q000378:metabolism
D015415:Biomarkers; D007375:Interleukin-1; D016209:Interleukin-8; D012333:RNA, Messenger; D009195:Peroxidase; D013482:Superoxide Dismutase