[Antigen analysis of Trichomonas vaginalis trophozoite by SDS-PAGE and two-dimensional gel electrophoresis].
OBJECTIVE
To analyze soluble antigens of Trichomonas vaginalis.


METHODS
Soluble antigens of the parasite from a patient suffering from trichomonad vaginitis were analyzed by SDS-PAGE, immunoblotting and two-dimensional gel electrophoresis.


RESULTS
A total of 26 distinct protein bands were demonstrated by using 10% resolution gel. Nine of them were main bands, eight with MWs 15-62 kDa, one with MW 97 kDa. By immunoblotting the specific anti-T. vaginalis antibodies raised in rabbit recognized 24 protein bands with 8 main bands in them. Two-dimensional gel electrophoresis revealed up to 43 individual trichomonad polypeptide spots, among which, 9 were main ones. The pI and MWs of these spots were 3.65-5.84 and 27-->100 kDa respectively.


CONCLUSIONS
Eight protein bands out of 26 soluble antigen bands of the parasite showed high immunogenicity. There were 9 main polypeptide spots in 43 polypeptide spots of the parasite.
Gao
X Z
XZ
Department of Parasitology, School of Basic Medical Sciences, Peking University, Beijing 100083.
Tam
J W
JW
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D015151:Immunoblotting; D014247:Trichomonas Vaginitis / Q000469:parasitology; D014246:Trichomonas vaginalis / Q000276:immunology* / Q000302:isolation & purification
D000953:Antigens, Protozoan