[Laboratory diagnosis of Acanthamoeba keratitis].
OBJECTIVE
To find a rapid method for diagnosing Acanthamoeba keratitis and identifying Acanthamoeba.


METHODS
10% potassium hydroxide (KOH) wet-mount preparations, Acanthamoeba culture, inverted phase contrast microscopy, and pathological examination using H. E. staining and PAS staining.


RESULTS
Using corneal scrapings and corneal materials obtained from surgery, 7 cases and 5 cases of Acanthamoeba keratitis were diagnosed by 10% KOH wet-mount preparations. 6 strains of Acanthamoeba were isolated in corneal materials of 6 cases by protozoa culture method. The cysts, trophozoites and pseudopods on the trophozoites of Acanthamoeba were directly observed under the inverted phase contrast microscope. The cysts and trophozoites of Acanthamoeba were seen by H. E. staining and PAS staining with 20 h.


CONCLUSIONS
Acanthamoeba keratitis could be rapidly diagnosed by 10% KOH wet-mount preparations and inverted phase contrast microscopy. Acanthamoeba organisms could be directly observed and identified under inverted phase contrast microscope.
Deng
X G
XG
Henan Institute of Ophthalmology, Zhengzhou 450003.
Li
J C
JC
Zhu
L
L
D000048:Acanthamoeba / Q000302:isolation & purification*; D015823:Acanthamoeba Keratitis / Q000175:diagnosis* / Q000469:parasitology; D000818:Animals; D003315:Cornea / Q000469:parasitology; D006801:Humans; D006878:Hydroxides; D008853:Microscopy; D017680:Potassium Compounds; D013194:Staining and Labeling
D006878:Hydroxides; D017680:Potassium Compounds; C029943:potassium hydroxide