[Overexpression of Bcl-2 inhibited T lymphocyte apoptosis mediated by CD3 epsilon molecule].
OBJECTIVE
To study the role of bcl-2 gene overexpression in T lymphocyte apoptosis mediated by CD8 epsilon chimera molecule.


METHODS
The cells expressed CD8 epsilon chimera molecule were transfected with a bcl-2 expression vector. The cells with Bcl-2 overexperssion were selected by G418 and limited dilution, and then treated with anti-CD8 antibody. The cell death was evaluated with flow cytometry.


RESULTS
It was shown that there were only 2.6% of cells with Bcl-2 overexpression died by apoptosis. Comparing with Jurkat and TJK cells without Bcl-2 expression, in which there were 6.1% and 33.7% of cell death respectively, suggesting that Bcl-2 overexpression remarkbly inhibited apoptosis of the T lymphocytes mediated by CD8 epsilon chimera molecule.


CONCLUSIONS
Bcl-2 overexpression could block T lymphocyte apoptosis mediated by CD3 epsilon molecule and the mechanisms remained to be clarified in detail.
Yang
X
X
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Liu
Y
Y
Zheng
D
D
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017209:Apoptosis; D017252:CD3 Complex; D016827:CD8 Antigens / Q000276:immunology; D015870:Gene Expression; D019254:Genes, bcl-2; D006801:Humans; D019169:Jurkat Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis*; D014162:Transfection
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; C115335:CD3E protein, human; D016827:CD8 Antigens; D019253:Proto-Oncogene Proteins c-bcl-2; D011948:Receptors, Antigen, T-Cell