[Regulative effects of bailong and Hexamethylene bisacetamide on growth and phenotype of human gastric cancer cells in cell cycle].
OBJECTIVE
To investigate and compare the common effect of Bailong, a Chinese herbal preparation, and Hexamethylene bisacetamide (HMBA), a western cell differentiation inducer, on growth regulation and phenotype of human gastric cancer (MGC80-3) cells in different phases of cell cycle.


METHODS
Synchronized MGC80-3 cells in monolayer cultures with nitrous oxide under high pressure were double blocked with overdosage of TdR in the 4 cellular phases (G1, S, G2 and M), and then collected and treated with Bailong and 5 mmol/L HMBA respectively.


RESULTS
Both medicines could suppress the cell proliferation, soft agar cloning growth and microfilament assembling of different phases of cells, the effect on cells of G1 phase was the most significant one.


CONCLUSIONS
Bailong and HMBA had significant common characteristics in inducing cell differentiation of MGC80-3 cells in different phases of cell cycle.
Li
B Y
BY
Beijing Institute for Cancer Research, School of Clinical Oncology, Peking University, Beijing 100034.
Liang
Y Y
YY
Wang
D S
DS
D000081:Acetamides / Q000494:pharmacology*; D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D010641:Phenotype; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000081:Acetamides; D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; C014026:hexamethylene bisacetamide