[Effect of recombinant prothymosin alpha on secretion of IFN-gamma, IFN-alpha and TNF-alpha in vitro].
OBJECTIVE
To study the effect of prothymosin alpha (Pro T alpha) as a fusion protein on secretion of IFN-gamma, IFN-alpha and TNF-alpha in vitro.


METHODS
The in vitro study was carried out on the culture of splenocytes, splenic and peritoneal macrophages isolated from Balb/c mice. Splenocytes were incubated with various concentrations of Pro T alpha (1 x 10(-7)-1 x 10(-10) mol.L-1) with or without Con A (5 micrograms.mL-1) for 72 h. Splenic and peritoneal macrophages were respectively treated with Pro T alpha (1 x 10(-7)-1 x 10(-10) mol.L-1) in the presence of LPS (10 micrograms.mL-1) for 24 h. Then IFN-gamma, IFN-alpha and TNF-alpha levels in the supernatant were detected by ELISA.


RESULTS
Pro T alpha (1 x 10(-7) mol.L-1) was found to obviously increase IFN-gamma level (P < 0.05) in the supernatant of splenocytes compared with the control group. Moreover, Pro T alpha (1 x 10(-7) mol.L-1) significantly induced the secretion of IFN-alpha (P < 0.01) and TNF-alpha (P < 0.01) in splenic and peritoneal macrophages.


CONCLUSIONS
In vitro, Pro T alpha could increase the secretion of IFN-gamma, IFN-alpha and TNF-alpha.
Qiu
Lei
L
Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Guo
Bao-yu
BY
Miao
Hong
H
Dao
Shu-yan
SY
Zhang
Ran
R
Yuan
Peng-qun
PQ
Yang
Xu
X
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D002469:Cell Separation; D005260:Female; D005982:Glutathione Transferase / Q000494:pharmacology; D016898:Interferon-alpha / Q000378:metabolism*; D007371:Interferon-gamma / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011498:Protein Precursors / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D013947:Thymosin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D016898:Interferon-alpha; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha; C040782:prothymosin alpha; D013947:Thymosin; D007371:Interferon-gamma; D005982:Glutathione Transferase