Further isolation of antioxidative (+)-1-hydroxypinoresinol-1-O-beta-D-glucoside from the rhizome of Salvia miltiorrhiza that acts on peroxynitrite, total ROS and 1,1-diphenyl-2-picrylhydrazyl radical.
A furanofuranoid lignan glycoside, with radical scavenging on peroxynitrite, total reactive oxygen species (ROS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, was isolated from the rhizome of Salvia miltiorrhiza and characterized as (+)-1-hydroxypinoresinol-1-O-beta-D-glucoside based on spectroscopic evidence. The compound exhibited peroxynitrite, total ROS and DPPH radical scavenging activities with IC50 values of 3.23 +/- 0.04, 2.26 +/- 0.07 and 32.3 +/- 0.13 microM, respectively. Penicillamine, Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) and L-ascorbic acid, acting as positive controls, showed radical scavenging activities with IC50 values of 6.72 +/- 0.25, 1.43 +/- 0.04 and 11.4 +/- 0.07 microM, respectively.
Kang
Hye Sook
HS
Faculty of Food Science and Biotechnology, Pukyong National University, Pusan 608-737, Korea.
Chung
Hae Young
HY
Byun
Dae Seok
DS
Choi
Jae Sue
JS
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001713:Biphenyl Compounds; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D030421:Peroxynitrous Acid / Q000378:metabolism*; D010851:Picrates / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D027343:Rhizome / Q000737:chemistry / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000737:chemistry / Q000378:metabolism*
C435257:1-hydroxypinoresinol 1-O-glucopyranoside; D000975:Antioxidants; D001713:Biphenyl Compounds; D005960:Glucosides; D010851:Picrates; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D030421:Peroxynitrous Acid; C004931:1,1-diphenyl-2-picrylhydrazyl