title: Interleukin-1 facilitates airway epithelial migration in response to injury.
abstract: OBJECTIVE
  To test the hypothesis that interleukin (IL)-1 plays a permissive role in respiratory epithelial cell migration and proliferation.
  
  
  METHODS
  Primary cultures of porcine respiratory epithelial cells or tracheal organ explants were cultured in the presence or absence of function-blocking antibodies to IL-1. Areas of epithelial cell outgrowth were determined in control and antibody-treated organ explants daily for 4 days. At intervals, cultured cells were collected for cell counting and viability determination. Time course and dose-response curves were constructed for control and antibody-treated groups.
  
  
  RESULTS
  Interleukin-1 secretion into culture supernatants increased sharply from days 3 to 7. Outgrowths from tracheal explants were reduced by greater than 60% by single antibody treatment, and by over 90% by treatment with antibodies to both IL-1alpha and -1beta by day 4 of culture. Function-blocking antibodies to IL-1 significantly reduced cell number by day 7 of culture.
  
  
  CONCLUSIONS
  Interleukin-1 is produced by respiratory epithelial cells in culture during log phase growth and plays a permissive role in cell migration and proliferation.
authors.affiliation: Department of Surgical Oncology, Head and Neck Surgery, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA. Wesley.Hicks@roswellpark.org
authors.forename: Wesley
authors.initials: W
authors.lastname: Hicks
authors.forename: Leon A
authors.initials: LA
authors.lastname: Hall
authors.forename: Debra A
authors.initials: DA
authors.lastname: Tristram
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Gardella
authors.forename: Frank V
authors.initials: FV
authors.lastname: Bright
authors.forename: Robert
authors.initials: R
authors.lastname: Hard
authors.forename: Lynn
authors.initials: L
authors.lastname: Sigurdson
mesh_terms: D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000494:pharmacology; D002455:Cell Division / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D007375:Interleukin-1 / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D009924:Organ Culture Techniques; D020545:Respiratory Mucosa / Q000166:cytology* / Q000378:metabolism; D013552:Swine; D014132:Trachea / Q000166:cytology
chemical_list: D000906:Antibodies; D007375:Interleukin-1