[Expression of tyrosine kinase receptor B in human follicle].
OBJECTIVE
To study the expression of tyrosine kinase receptor B(TrkB) in human follicle and explore the significance of the expression.


METHODS
Streptavidin-biotin peroxidase complex immunocytochemistry was employed to determine the expression of TrkB on the oocytes, cumulus granulosa cells (CGCs) or mural granulosa cells (MGCs) obtained from women during controlled ovarian hyperstimulation for in vitro fertilization-embryo transfer (IVF-ET). Western blotting was used to analyze TrkB expression level in CGCs and MGCs.


RESULTS
The results demonstrated for the first time the presence of TrkB on the surface of human ovary cells including MGCs, CGCs and the oocytes. Higher expression levels of TrkB were detected on the MGCs than on the CGCs.


CONCLUSIONS
It is possible that TrkB tyrosine autophosphorylation in response to neurotrophins can take part in the regulation and modulation of steroidogenesis and oocyte maturation.
Fu
Juan
J
Center for Assisted Reproduction, Department of Obstetrics and Gynecology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. fj81609@sina.com.cn
Xing
Fu-qi
FQ
Chen
Shi-ling
SL
D000328:Adult; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D006080:Ovarian Follicle / Q000201:enzymology* / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis*; D020813:Receptor, trkB / Q000096:biosynthesis* / Q000235:genetics
D019208:Brain-Derived Neurotrophic Factor; D020794:Receptor Protein-Tyrosine Kinases; D020813:Receptor, trkB