[Study on immunomodulating mechanism of the active fraction of liuwei dihuang decoction].
OBJECTIVE
To investigate the immunomodulating mechanism of 3A, an active fraction extracted from the ethanol-soluble portion of Liuwei Dihuang Decoction (LWDHD).


METHODS
Influence of 3A on T-lymphocyte subsets, IgG production of B cells, interferon-gamma (IFN-gamma) mRNA expression in cyclophosphamide (Cy)-treated mice, tumor bearing mice and senescence accelerated mouse-prone/8 (SAMP8) were assessed in vivo and in vitro.


RESULTS
Oral administration of 3A could elevate the decreased T-lymphocyte subsets in all model mice, it also could promote the IgG secretion of splenic cells in SAMP8 and increase IFN-gamma mRNA expression in Cy-treated mice.


CONCLUSIONS
The immunomodulating effect of 3A is related with its effects in regulating ratio of T and B cells or in improving their functions.
Yang
S
S
Beijing Institute of Pharmacology and Toxicology, Academy of Military Medicine, Beijing 100850.
Zhang
Y X
YX
Lu
X D
XD
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D003520:Cyclophosphamide; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D016867:Immunocompromised Host / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012510:Sarcoma 180 / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000276:Adjuvants, Immunologic; D004365:Drugs, Chinese Herbal; D007074:Immunoglobulin G; C042579:Liuwei Dihuang Decoction; D012333:RNA, Messenger; D007371:Interferon-gamma; D003520:Cyclophosphamide