[Study of microsatellite polymorphisms between the Han nationality and French population by fluorescence-based genotype].
OBJECTIVE
To facilitate the application of the large scale genome scan and individual identification by investigating the differences of microsatellite polymorphisms of the genethon human genetic linkage map between the Han nationality and French population.


METHODS
Six to seventeen pairs of primers were added in one single tube with 5 microliters reaction volume and several microsatellite markers were amplified simultaneously. The products were electrophoresited on the 377XL DNA sequencer.


RESULTS
During the entire experiment 400 microsatellite loci were amplified in 32 tubes and 306 microsatellite loci were satisfactory.


CONCLUSIONS
The size range of genetic polymorphisms are different in race, and the difference exists at 124 microsatellite loci between the Han nationality and French population, so 10 bp should be added to both sides of the genethor map size range when we perform genotype in Han nationality.
Deng
Hao
H
National Laboratory of Medical Genetics, Central South University, Changsha 410078, China.
Lu
Chun-ye
CY
Zhang
Hua-li
HL
D000328:Adult; D044466:Asian People; D005006:Ethnicity; D005260:Female; D005453:Fluorescence; D033142:Genetic Services; D016678:Genome; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic / Q000235:genetics*; D044465:White People