[Structures and pharmacological activities of beesiosides O and P].
OBJECTIVE
To investigate the chemical constituents of the rhizomes of Beesia calthaefolia native to China in order to obtain a more comprehensive understanding of its effective components.


METHODS
Compounds were isolated by column chromatography with silica gel. Their structures were elucidated by spectral analysis and chemical evidence. Compounds identified were subjected to pharmacological evaluation.


RESULTS
Two novel compounds were isolated and identified as (20S, 24S)-15 alpha-acetoxy-16 beta, 24; 20, 24-diepoxy-9, 19-cyclolanostane-3 beta, 25-diol-3-O-beta-D-xylopyranoside (I) and (20S, 24R)-15 alpha-acetoxy-9, 19-cyclolanostane-3 beta, 16 beta, 20, 24, 25-pentaol-3-O-beta-D-xylopyranoside (II), named beesioside O and beesioside P.


CONCLUSIONS
Compounds I and II are new compounds. Compounds I exhibited immunosuppressive activity and could inhibit angiogenesis as well as inhibit the proliferation of osteoblast. Compound II displayed remarkable inhibition activity against calcium channel receptor.
Ju
Jian-Hua
JH
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China. junshanyang@hotmail.com
Lin
Geng
G
Yang
Jun-shan
JS
Lu
Hai-yan
HY
Ma
Bing-na
BN
Nie
Shu-qin
SQ
Zhang
Xia
X
D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002121:Calcium Channel Blockers / Q000737:chemistry / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D008968:Molecular Conformation; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D029626:Ranunculaceae / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
C486138:15-acetoxy-16,24-20,24-diepoxy-9,19-cyclolanostane-3,25-diol-3-O-xylopyranoside; C486139:15-acetoxy-9,19-cyclolanostane-3,16,20,24,25-pentaol-3-O-xylopyranoside; D020533:Angiogenesis Inhibitors; D002121:Calcium Channel Blockers; D007166:Immunosuppressive Agents; D012503:Saponins