The regulation of yp3 expression in the Drosophila melanogaster fat body.
The regulation of the Drosophila melanogaster yolk protein genes 1 and 2 have been well characterised. Cis-acting DNA elements and trans-acting factors regulating ovarian fat body and sex-specific expression have been identified. In this paper we have analysed the regulation of yolk protein 3, which is separated from the other two genes on the X-chromosome. We have separated sex-specific control from fat body control in some constructs in transgenic flies. We propose that the organisation of the regulatory elements in yp3 differs from yp1 and yp2 for control of fat body expression and that it closely resembles the regulation of a reporter gene using Musca and Calliphora yp promoter enhancer sequences in transgenic Drosophila.
Hutson
Simone F
SF
Institute of Cell and Molecular Biology, University of Edinburgh, King's Buildings, Edinburgh, EH9 3JR UK.
Bownes
Mary
M
D000818:Animals; D030801:Animals, Genetically Modified; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D004527:Egg Proteins / Q000235:genetics* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D024202:Electrophoretic Mobility Shift Assay; D005216:Fat Body / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D014818:Vitellogenesis / Q000235:genetics; D001616:beta-Galactosidase / Q000378:metabolism
D029721:Drosophila Proteins; D004527:Egg Proteins; C070799:YP3 protein, Drosophila; D001616:beta-Galactosidase