[Isolation and structure identification of ligan glycosides from pine needles of Pinus massoniana lamb].
OBJECTIVE
To study the chemical constituents of the pine needles of Pinus massoniana lamb..


METHODS
Various chromatographic techniques were used to separate and purify. Their physico-chemical properties and spectral data (UV, IR, MS, 1H-1 H COSY, HMQC, DEPT, HMBC and ORD ect.) were measured for structure elucication.


RESULTS
Three compounds were isolated from the n-BuOH fraction of water-extracts. Their structures were identified as massonianoside A (4), massonianoside A: (7S, 8R)-3, 4, 9'-trihydroxyl-3-methyoxyl-7, 8-dihydrobenzofunan-1'-propanolneoligan-9-O-alpha-L-rhamnopyranoside, massonianoside C (5), (7S, 8R)-9,9'-dihydroxyl-3,3'-dimethyoxyl-7,8-dihydrobenzofunan-1'- propanolneoligan-4-O-alpha-L-rhamnopyranoside and cedrusin-4-O-beta-glucoside (6), (7S, 8R)-3',9,9'-trihydroxyl-3-methoxyl-7,8-dihydrobenzofunan-1'- propanolneoligan-4-O-beta-D-glucopyranoside.


CONCLUSIONS
Compound 4 and 5 are new compounds.
Bi
Yue-feng
YF
Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China.
Zheng
Xiao-ke
XK
Feng
Wei-sheng
WS
Zhang
Yong-zhong
YZ
Ji
Chun-ru
CR
D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D028223:Pinus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
C484708:3,4,9'-trihydroxy-3-methoxy-7,8-dihydrobenzofuran-1'-propanolneoligan-9-O-rhamnopyranoside; C484709:9,9'-dihydroxy-3,3'-dimethoxy-7,8-dihydrobenzofuran-1'-propanolneoligan-4-O-rhamnopyranoside; D001572:Benzofurans; D006027:Glycosides