[Mechanism of sini decoction in regulating beta-adrenergic receptor signal transduction in rats with myocardial ischemia].
OBJECTIVE
To investigate the effect of Sini Decoction (SND) on beta-adrenergic receptor (beta-AR) signal transduction in rats with myocardial ischemia.


METHODS
After beta-AR had been blocked by propranolol, the myocardial ischemia rats were established by pituitrin injection. The density of beta-AR in myocardial cell membrane was determined by radioligand binding assay, cAMP level in plasma and myocardial tissue was detected by radioimmunoassay (RIA), and the mRNA expression of beta 1-AR and beta 1-AR kinase (beta ARK-1) was measured by quantitative reverse transcriptase polymerase chain reaction (RT-PCR) amplifying assay.


RESULTS
In myocardial ischemia group, the density of beta 1-AR was up-regulated and the mRNA expression of beta ARK-1 increased (P < 0.01), but the cAMP level in plasma and myocardium was lower than that in the normal group (P < 0.01 and P < 0.05). SND could promote beta 1-AR mRNA expression and inhibit beta ARK-1 mRNA expression so as to increase the density of beta-AR and cause significant raising of cAMP level in plasma and myocardium.


CONCLUSIONS
SND could reduce the desensitization of beta 1-AR during myocardial ischemia and improve signal transduction of beta-AR.
Tang
T J
TJ
Institute of Integrated Traditional Chinese and Western Medicine, Sun Yat-sen University of Medical Sciences, Guangzhou 510089.
Wu
W K
WK
Lu
H P
HP
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D008297:Male; D017202:Myocardial Ischemia / Q000139:chemically induced / Q000378:metabolism*; D010909:Pituitary Hormones, Posterior; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018343:Receptors, Adrenergic, beta-2 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction
D004365:Drugs, Chinese Herbal; D010909:Pituitary Hormones, Posterior; D012333:RNA, Messenger; D018343:Receptors, Adrenergic, beta-2