[Effects of vasoactive intestinal peptide on the enzyme activity of CTP: phosphorylcholine cytidylyltransferase from the rat lung].
OBJECTIVE
To investigate the effect of vasoactive intestinal peptide(VIP) on the microsomal CTP: phosphorylcholine cytidylyltransferase activity of cultured lung explants and its mechanisms.


METHODS
The CDP-[M-14 C] choline content reflecting the microsomal CTP: phosphorylcholine cytidylyltransferase activity of cultured lung explants was measured with liquid scintillation.


RESULTS
1. VIP increased the microsomal CTP: phosphorylcholine cytidylyltransferase activity of cultured lung explants in a dose-dependence manner and in a time-dependence fashion; 2. The effect of 3 x 10(-7) mol.L-1 VIP on the microsomal CTP: phosphorylcholine cytidylyltransferase activity of cultured lung explants was inhibited by H-7, a protein kinase C(PKC) inhibitor and W-7, a calmodulin antagonist respectively.


CONCLUSIONS
VIP in the lung may be one of the key regulators involved in the synthesis of phosphatidylcholine mediated by PKC and calmodulin.
Guan
Cha-xiang
CX
Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Zhou
Fu-wen
FW
Luo
Zi-qiang
ZQ
D000818:Animals; D002147:Calmodulin / Q000378:metabolism; D002478:Cells, Cultured; D019916:Choline-Phosphate Cytidylyltransferase / Q000378:metabolism*; D005260:Female; D008168:Lung / Q000166:cytology / Q000201:enzymology*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D002147:Calmodulin; D014660:Vasoactive Intestinal Peptide; D011493:Protein Kinase C; D019916:Choline-Phosphate Cytidylyltransferase