Herbal remedies traditionally used against malaria in Ghana: bioassay-guided fractionation of Microglossa pyrifolia (Asteraceae).
Different extracts from 11 West African plants traditionally used against malaria in Ghana were tested against both the chloroquine-sensitive strain PoW and the chloroquine-resistant clone Dd2 of Plasmodium falciparum. Due to the promising in vitro activity of the lipophilic extract [IC50: 10.5 microg/ml (PoW); 13.1 microg/ml (Dd2)], Microglossa pyrifolia (Lam.) Kuntze (Asteraceae) was chosen for further phytochemical investigation. From active fractions 13 compounds were isolated; their structures were established on the basis of spectroscopic methods. 1-Acetyl-6E-geranylgeraniol-19-oic acid and sinapyl diangelate represent new natural compounds. The two diterpenes E-phytol [IC50: 8.5 microM (PoW); 11.5 microM (Dd2)], and 6E-geranylgeraniol-19-oic acid [IC50: 12.9 microM (PoW); 15.6 microM (Dd2)] proved to be the most active constituents in our test system.
Köhler
Inga
I
Institut für Pharmazie (Pharmazeutische Biologie), Freie Universität Berlin, Königin-Luise-Strasse 2+4, D-14195 Berlin, Germany.
Jenett-Siems
Kristina
K
Kraft
Carola
C
Siems
Karsten
K
Abbiw
Daniel
D
Bienzle
Ulrich
U
Eich
Eckart
E
D000962:Antimalarials / Q000737:chemistry* / Q000627:therapeutic use; D019659:Asteraceae / Q000737:chemistry*; D001681:Biological Assay; D005869:Ghana; D029001:Herbal Medicine; D006801:Humans; D008288:Malaria / Q000188:drug therapy*; D008517:Phytotherapy; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification* / Q000627:therapeutic use*
D000962:Antimalarials; D010936:Plant Extracts