title: [Effects of rhGM-CSF on Apoptosis of HL-60 Cells Induced with VP-16 Treatment]
abstract: In order to investigate the effects of rhGM-CSF on apoptosis of HL-60 cells induced with VP-16 treatment, HL-60 cells were first incubated with rhGM-CSF before they were treated with VP-16. The apoptosis processes and the changes in apoptosis related gene bcl-2 and fas expression were observed. The morphological and ultrastructural changes were observed under optics microscope and electromicroscope. DNA fragmentation were detected by agarose gel electrophoresis, the apoptotic rate, bcl-2 and fas expression with flow cytometry. Our results showed that rhGM-CSF inhibited the apoptosis of HL-60 cells induced with VP-16 treatment, which enhanced the down-regulation of bcl-2 expression, but inhibited the up-regulation of fas expression. So it suggested that rhGM-CSF can decrease the sensitivity of HL-60 cells to VP-16, probably by down-regulation of fas expression.
authors.affiliation: Beijing Tongren Hospital. Capital University of Medical Science, Beijing 100730, China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Liu
authors.forename: Xinqiang
authors.initials: X
authors.lastname: Jin
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
authors.forename: Jinjin
authors.initials: J
authors.lastname: Wang
authors.forename: Yiping
authors.initials: Y
authors.lastname: Wu
authors.forename: Yanling
authors.initials: Y
authors.lastname: Zhai
authors.forename: Yuanbo
authors.initials: Y
authors.lastname: Liu