[Twin test in the determination of alanine aminotransferase and aspartate aminotransferase in the serum].
OBJECTIVE
To explore a method which can simultaneously determine alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum.


METHODS
Using the self-prepared twin test reagent, ALT and AST in the serum were detected simultaneously.


RESULTS
There was a good correlation between the twin test's method and the traditional method in the detection of ALT and AST (All r = 0.999). The precision of the method was good, and the coefficient variation (CV) was below 5%. Hemolysis, jaundice, and lipohemia serum were found to be interference-free. The time of the detection was two times of the traditional method.


CONCLUSIONS
The twin test method can both exert the capability of the automatic analyzer and shorten the time of detection. It is a method worthy to be popularized.
Jiang
Hong-min
HM
Department of Clinical Laboratory, Second Xiangya Hospital, Central South University, Changsha 410011, China.
D000410:Alanine Transaminase / Q000097:blood*; D001219:Aspartate Aminotransferases / Q000097:blood*; D006801:Humans; D012680:Sensitivity and Specificity; D013997:Time Factors
D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase