ATP consumption rate per cross bridge depends on myosin heavy chain isoform.
In the present study, we tested the hypothesis that intrinsic differences in ATP consumption rate per cross bridge exist across rat diaphragm muscle (Dia(m)) fibers expressing different myosin heavy chain (MHC) isoforms. During maximum Ca(2+) activation (pCa 4.0) of single, Triton X-permeabilized Dia(m) fibers, isometric ATP consumption rate was determined by using an NADH-linked fluorometric technique. The MHC concentration in single Dia(m) fibers was determined by densitometric analysis of SDS-PAGE gels and comparison to a standard curve of known MHC concentrations. Isometric ATP consumption rate varied across Dia(m) fibers expressing different MHC isoforms, being highest in fibers expressing MHC(2X) (1.14 +/- 0.08 nmol. mm(-3). s(-1)) and/or MHC(2B) (1.33 +/- 0.08 nmol. mm(-3). s(-1)), followed by fibers expressing MHC(2A) (0.77 +/- 0.11 nmol. mm(-3). s(-1)) and MHC(Slow) (0.46 +/- 0.03 nmol. mm(-3). s(-1)). These differences in ATP consumption rate also persisted when it was normalized for MHC concentration in single Dia(m) fibers. Normalized ATP consumption rate for MHC concentration varied across Dia(m) fibers expressing different MHC isoforms, being highest in fibers expressing MHC(2X) (2.02 +/- 0.19 s(-1)) and/or MHC(2B) (2.64 +/- 0.15 s(-1)), followed by fibers expressing MHC(2A) (1.57 +/- 0.16 s(-1)) and MHC(Slow) (0.77 +/- 0.05 s(-1)). On the basis of these results, we conclude that there are intrinsic differences in ATP consumption rate per cross bridge in Dia(m) fibers expressing MHC isoforms.
Han
Young-Soo
YS
Department of Anesthesiology, Mayo Medical School, Rochester, Minnesota 55905, USA.
Geiger
Paige C
PC
Cody
Mark J
MJ
Macken
Rebecca L
RL
Sieck
Gary C
GC
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D003964:Diaphragm / Q000378:metabolism; D004734:Energy Metabolism; D007537:Isometric Contraction / Q000502:physiology; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism*; D009994:Osmolar Concentration; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D020033:Protein Isoforms; D000255:Adenosine Triphosphate; D018995:Myosin Heavy Chains