[The T cell activation from peripheral blood of patients with herpetic keratitis following in vitro activation].
OBJECTIVE
To evaluate the T cells activation from peripherial blood(PB) of patients with herpes simplex keratitis following in vitro activation.


METHODS
The flow cytometry was used to investigate the expression of HLA-DR, CD25, CD69, CD71 on T cell from PB, the PB in culture, the PB cultured with phytohemagglutinin(PHA) or phorbol 12, 13-dibutyrate(PDB) or PDB and ionophore (IONO), in 7 cases with herpetic stromal keratitis (HSK) and 4 cases with herpetic ulcer keratitis(HUK). 6 normal people were set as control.


RESULTS
The percentages of CD3+DR71+ was higher, and the CD3+CD71+ was lower from PB of patients with HSK than those from normal people. After the PB was cultured for 72 hours, the percentages of CD8+CD71+ in lymphocytes from HSK and CD8+DR+, CD8+CD69+, CD8+CD71+ in lymphocytes from HUK were lower than those from nomal people. And the CD8+CD71+ cells from HUK also was lower than those from HSK. When the PB was cultured with PHA for 72 hours, the percentages of CD3+CD71+, CD8+CD69+, CD8+CD71+ from HSK and HUK were lower than those from normal people. The percentages of CD8+CD25+ in lymphocytes from HUK also was lower than those from normal, and the CD8+CD69+ cells in lymphocytes was lower than those from HSK. The percentages of CD3+CD71+ in PB cultured with PDB for 72 hours, CD3+CD69+ in PB cultured with PDB and IONO for 72 hours from HSK were lower than those from normal people.


CONCLUSIONS
The ability of T cells activation decreases in cases with HSK following in vitro activation. The lymphocytes in patients with HUK shows much lower activation than those cases with HUK.
Liu
Z
Z
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guanmgzhou 510060, Cnina.
Chen
J
J
Li
S
S
Zeng
Y
Y
Sun
H
H
D015703:Antigens, CD / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D016849:Keratitis, Herpetic / Q000276:immunology*; D037181:Lectins, C-Type; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D006684:HLA-DR Antigens; D037181:Lectins, C-Type; D000099192:CD69 Antigens