[Establishment of immune insulin resistance model in the rats by i.v. injection of BCG].
OBJECTIVE
To observe the stability of BCG-induced insulin resistance model.


METHODS
The glucose tolerance, serum glucose, FFA, insulin, triglycerides, cholesterol, TNF-alpha and ALT level were measured. The change of GDR was measured by euglycemic clamp in model rats after given i.v. BCG 2, 4 and 8 weeks.


RESULTS
After 2, 4 and 8 weeks, the GIR and glucose tolerance of the animals deceased significantly. After 2, 4 and 8 weeks, BCG infusion resulted in a pronounced reduction in glucose tolerance and insulin-stimulated glucose disposal rate [GDR = GDR: (29 +/- 6) vs (13 +/- 7) mg.kg-1.min-1 2 weeks; (29 +/- 6) vs (11 +/- 7) mg.kg-1.min-1 4 weeks and (23 +/- 3) vs (16 +/- 3) mg.kg-1.min-1 8 weeks, respectively, P < 0.01]. BCG infusion resulted in a pronounced increase in the weights of the liver [(6.2 +/- 0.9) vs (8.2 +/- 1.3) g, P < 0.05] and spleens [(0.51 +/- 0.11) vs (1.4 +/- 0.4) g, P < 0.01]. The histo-pathological results showed that BCG infusion resulted severe inflammation in the livers and spleens and the ratio of beta/alpha in pancreas increased. The serum levels of triglyceride, FFA and glucose were unchanged, but the level of serum TNF-alpha [543 +/- 60) vs (759 +/- 137) pg.mL-1, P < 0.05] and insulin [(31 +/- 5) vs (36 +/- 5) mu.L-1, P > 0.05] increased.


CONCLUSIONS
This novel model of immune insulin resistance is completely and constantly established.
Guo
Yu-xiao
YX
Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing 100083, China.
Luo
Mou-lun
ML
Lin
Zhi-bin
ZB
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003920:Diabetes Mellitus / Q000378:metabolism; D015309:Glucose Clamp Technique; D005951:Glucose Tolerance Test; D007275:Injections, Intravenous; D007328:Insulin / Q000097:blood*; D007333:Insulin Resistance / Q000276:immunology*; D008297:Male; D009163:Mycobacterium bovis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D001786:Blood Glucose; D007328:Insulin; D014409:Tumor Necrosis Factor-alpha