[Effect of 17 beta-estradiol on activity and gene expression of fibrinolytic factor in human umbilical vein endothelial cell].
OBJECTIVE
To study the effect of 17 beta-estrodial on fibrinolytic factor in human umbilical vein endothelical cell (HUVEC).


METHODS
When different concentration of 17 beta-estrodial including 10(-10) mol/L (the group E1), 10(-8) mol/L (the group E2), 10(-6) mol/L (the group E3) were treated in original cultured HUVEC, the changes of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI-1) were measured by spectrophotometric assay, and the level of message RNA were examined by in situ hybridization. The changes of intracellular free calcium concentration([Ca2+]i) in single cells were observed by kinetic observation with Laster Confocal Microscope.


RESULTS
The t-PA activity both in E1 and E2 were higher than control (P < 0.05 respectively). The t-PA expression in E1, E2, and E3 were increased. The concentration of intracellular free calcium had an elevated and declined curve under the supraphysiological doses of estrodial and declined in incubation with 17 beta-eatrodial at 10(-6) mol/L.


CONCLUSIONS
These suggeste that the protection of 17 beta-estrodial on cardivascular system is associated with high fibrinolytic activity and t-PA expression as well as with the change of [Ca2+]i.
Zhang
Y
Y
Department of Pathophysiology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Zhu
G
G
Duan
Y
Y
D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005342:Fibrinolysis; D015870:Gene Expression; D006801:Humans; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis* / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism
D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; D004958:Estradiol; D010959:Tissue Plasminogen Activator; D002118:Calcium