Trophic and steroidogenic effects of water deprivation on the adrenal gland of the adult female rat.
The effects of a 3-day water deprivation were studied in adult female rats in order to know what are the different zones of the adrenal gland and the hormonal factors involved in the growth and the activity of the adrenal gland. Water deprivation significantly increased plasma renin activity (PRA), plasma Angiotensin II (AII), vasopressin (AVP), epinephrine, aldosterone and corticosterone concentrations but did not modify the plasma adrenocorticotropin hormone (ACTH) level. Water deprivation significantly increased the absolute weight of the adrenal capsule containing the zona glomerulosa without modification of the density of cells per area unit suggesting that the growth of the adrenal capsule was due to a cell hyperplasia of the zona glomerulosa. Water deprivation significantly increased the density of AII type 1 (AT(1)) receptors in the adrenal capsule but did not modify the density of AII type 2 (AT(2)) receptors in the adrenal capsule and core containing the zona fasciculata, the zona reticularis and the medulla. The treatment of dehydrated female rats with captopril, which inhibits the angiotensin converting enzyme (ACE) in order to block the production of AII, significantly decreased the absolute weight of the adrenal capsule, plasma aldosterone and the density of AT(1) receptors in the adrenal capsule. The concentration of corticosterone in the plasma, the density of AT(2) receptors and the density of cells per unit area in the zona glomerulosa of the adrenal capsule were not affected by captopril-treatment. In conclusion, these results suggest that AII seems to be the main factor involved in the stimulation of the growth and the secretion of aldosterone by the adrenal capsule containing the zona glomerulosa during water deprivation. The low level of plasma ACTH is not involved in the growth of the adrenal gland but is probably responsible for the secretion of corticosterone by the zona fasciculata.
Chatelain
Denis
D
Service d'Anatomie et de Cytologie pathologiques, Hôpital Nord, F-80034 Amiens cedex, France.
Montel
Valérie
V
Dickes-Coopman
Anne
A
Chatelain
Alain
A
Deloof
Sylvie
S
D000311:Adrenal Glands / Q000502:physiology*; D000324:Adrenocorticotropic Hormone / Q000097:blood; D000450:Aldosterone / Q000097:blood; D000804:Angiotensin II / Q000097:blood; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000818:Animals; D002216:Captopril / Q000494:pharmacology; D003345:Corticosterone / Q000097:blood; D004837:Epinephrine / Q000097:blood; D005260:Female; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D012083:Renin / Q000097:blood; D013256:Steroids / Q000097:blood*; D013312:Stress, Physiological / Q000097:blood / Q000378:metabolism; D014667:Vasopressins / Q000097:blood; D014868:Water Deprivation / Q000502:physiology*
D000806:Angiotensin-Converting Enzyme Inhibitors; D013256:Steroids; D014667:Vasopressins; D000804:Angiotensin II; D000450:Aldosterone; D000324:Adrenocorticotropic Hormone; D002216:Captopril; D012083:Renin; D003345:Corticosterone; D004837:Epinephrine