[Experimental study of protective effect of shenmai injection on endotoxin induced systemic inflammatory reaction syndrome and multiple organ dysfunction syndrome].
OBJECTIVE
To study the protective effect of Shenmai injection (SMI) on systemic inflammatory reaction syndrome (SIRS) and multiple organ dysfunction syndrome (MODS) in animal model of SIRS, MODS induced by lipopolysaccharide (LPS).


METHODS
Male BALB/c mice, 5-7 weeks old were randomly divided into 3 groups, the normal control group, the LPS model group and the LPS + SMI group. The rectal temperature, peripheral white blood cell count (WBC), blood biochemical examination, histopathologic changes of lung and liver as well as the blood levels of endotoxin and tumor necrosis factor alpha (TNF alpha) were determined before and after treatment.


RESULTS
LPS could induce endotoxemia and high serum level of TNF alpha, decrease the rectal temperature and WBC, reduce blood glucose and increase serum triglyceride and cholesterol levels. Pathological examination showed that LPS could cause pulmonary alveolar congestion, edema, exudation, capillary dilation and inflammatory cell infiltration in liver and lung tissue. SMI could significantly raise the low body temperature caused by LPS and reduction of WBC, improve the hypoglycemia and high plasma TNF alpha level, alleviate the pathologic changes in organs and reduce the plasma level of LPS (endotoxin).


CONCLUSIONS
SMI has marked effect in protecting LPS caused SIRS, MODS, the mechanism might be related with the lowering of LPS (endotoxin) level and reducing of TNF alpha secretion.
Wei
Y L
YL
Beijing China-Japan Friendship Hospital, Beijing 100029.
Li
Y J
YJ
Liu
X
X
D000818:Animals; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004731:Endotoxins / Q000097:blood; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009102:Multiple Organ Failure / Q000097:blood / Q000139:chemically induced / Q000473:pathology*; D005894:Panax; D011897:Random Allocation; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood / Q000139:chemically induced / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D004731:Endotoxins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C009252:fructus schizandrae, radix ginseng, radix ophiopogonis drug combination