[Inhibitory effects of isorhapotigenin on IL-8 production and mRNA expression induced with TNF alpha in normal human synovial cells].
OBJECTIVE
To study the effects of isorhapotigenin (Iso) on interleukin-8 (IL-8) production and mRNA expression in normal human synovial cells (HSC) induced with TNF alpha.


METHODS
IL-8 were assayed with RIA method. The mRNA expression of IL-8 was detected by RT-PCR method.


RESULTS
It was shown that TNF alpha at concentrations of 0.05 to 0.5 U.mL-1 for 24 h significantly increased IL-8 production. The expression of IL-8 mRNA was also promoted by TNF alpha (0.25 U.mL-1) for 6 h. Iso at the concentrations of 1 x 10(-6) mol.L-1 to 1 x 10(-5) mol.L-1 showed inhibitory effects on IL-8 production induced with TNF alpha (0.25 U.mL-1). The further study indicated that Iso at the concentrations of 1 x 10(-6) mol.L-1 to 1 x 10(-5) mol.L-1 inhibited IL-8 mRNA expression in HSC induced with TNF alpha (0.25 U.mL-1).


CONCLUSIONS
TNF alpha promoted IL-8 production and mRNA expression in HSC. Iso inhibited IL-8 production and mRNA expression induced by TNF alpha (0.25 U.mL-1). This might be one of the anti-inflammatory mechanisms of Iso.
Hou
Q
Q
Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.
Li
L C
LC
Guo
Y
Y
Cheng
G F
GF
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D004347:Drug Interactions; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000494:pharmacology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016209:Interleukin-8; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha