[The influence of vagus nerve stimulation on NMDAR1 mRNA and GABAAR alpha 1 mRNA in thalamic reticular neucus of pentylenetetrazole-induced epileptic rats].
To study the antiepileptic mechanism of vagus nerve stimulation (VNS), we used the methods of in situ hybridyzation and image analysis to detect the expression of NMDAR1 mRNA and GABAA receptor alpha 1 subunit mRNA (GABAAR alpha 1 mRNA) in the thalamic reticular nuclus. The results show that the NMDAR1 mRNA expression of rats administered pentylenetetrazole(PTZ) is higher than that of control group. By treating with VNS, it decreased. On the contrary, the expression of GABAAR alpha 1 mRNA in the thalamic reticular nuclus of PTZ group rats is lower than that of control group. For rats treated with VNS, it increased. Therefore, it is concluded that VNS may reduce the excitability of cerebral cortices by depressing the activities of glutamic acid receptors (GluR) and by promoting the activities of gamma-aminobutyric acid receptors(GABAR) in thalamic reticular nuclus. So the formation and development of seizures are inhibited.
Zhang
Junjian
J
Department of Neurology, Zhongnan Hospital, Wuhan University, Wuhan 430071.
Zhang
Jinhua
J
D000818:Animals; D004599:Electric Stimulation Therapy / Q000379:methods*; D004827:Epilepsy / Q000139:chemically induced / Q000628:therapy*; D020646:Intralaminar Thalamic Nuclei / Q000378:metabolism*; D008297:Male; D010433:Pentylenetetrazole / Q000633:toxicity; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000096:biosynthesis* / Q000235:genetics; D016194:Receptors, N-Methyl-D-Aspartate / Q000096:biosynthesis* / Q000235:genetics; D014630:Vagus Nerve / Q000503:physiopathology
D010433:Pentylenetetrazole; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D016194:Receptors, N-Methyl-D-Aspartate