title: Homozygous deletions and point mutations of the Rit1/Bcl11b gene in gamma-ray induced mouse thymic lymphomas.
abstract: Allelic loss (LOH) mapping and sequence analysis were conducted for gamma-ray induced mouse thymic lymphomas and a novel tumor suppressor gene, Rit1/Bcl11b, on chromosome 12 was isolated. Bi-allelic changes were found in 17 of the 66 p53-proficient lymphomas with Rit1 LOH but in only 2 of the 54 p53-deficient lymphomas. This suggests an association between the presence of functional p53 and inactivation of the Rit1 gene in the lymphoma development. Introduction of Rit1 into HeLa cells lacking Rit1 expression suppressed cell growth. These results indicate that loss-of-function mutations of Rit1 contribute to mouse lymphomagenesis and possibly to human cancer development.
authors.affiliation: Department of Gene Regulation, Graduate School of Medical and Dental Sciences, Niigata University, Asahimachi 1-757, Niigata 951-8122, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Wakabayashi
authors.forename: Jun
authors.initials: J
authors.lastname: Inoue
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Matsuki
authors.forename: Hitomi
authors.initials: H
authors.lastname: Kosugi-Okano
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Shinbo
authors.forename: Yukio
authors.initials: Y
authors.lastname: Mishima
authors.forename: Ohtsura
authors.initials: O
authors.lastname: Niwa
authors.forename: Ryo
authors.initials: R
authors.lastname: Kominami
mesh_terms: D000483:Alleles; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002460:Cell Line; D005260:Female; D005720:Gamma Rays; D017353:Gene Deletion; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity; D008223:Lymphoma / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009381:Neoplasms, Radiation-Induced / Q000235:genetics*; D017354:Point Mutation; D013953:Thymus Neoplasms / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D018631:ras Proteins / Q000235:genetics* / Q000502:physiology
chemical_list: D016159:Tumor Suppressor Protein p53; C486776:RIT1 protein, human; C472865:Rit1 protein, mouse; D018631:ras Proteins