Detection of Helicobacter pylori in paraffin-embedded and in shock-frozen gastric biopsy samples by fluorescent in situ hybridization.
We report on the successful application of fluorescent in situ hybridization for detection of Helicobacter pylori and determination of its clarithromycin susceptibility in formalin-fixed and paraffin-embedded gastric biopsy specimens that had been prepared for pathological examination. This method is useful when results from conventional culturing with antibiotic susceptibility testing are not available.
Rüssmann
Holger
H
Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians-Universität München, 80336 Munich, Germany. ruessmann@m3401.mpk.med.uni-muenchen.de
Feydt-Schmidt
Anne
A
Adler
Kristin
K
Aust
Daniela
D
Fischer
Almuth
A
Koletzko
Sibylle
S
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001706:Biopsy; D017291:Clarithromycin / Q000494:pharmacology; D016481:Helicobacter Infections / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000187:drug effects / Q000302:isolation & purification*; D006652:Histological Techniques; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008826:Microbial Sensitivity Tests; D016612:Paraffin Embedding; D012680:Sensitivity and Specificity; D013270:Stomach / Q000382:microbiology* / Q000473:pathology
D000900:Anti-Bacterial Agents; D017291:Clarithromycin