[Gene typing of merozoite surface protein 1 of Plasmodium falciparum isolates from Hainan Province].
OBJECTIVE
To identify the genotype of merozoite surface protein 1 (MSP1) of Plasmodium falciparum isolates from Hainan Province.


METHODS
Nested PCR was applied to amplify the MSP1 of Blocks 2 and 3 Plasmodium falciparum isolates from Hainan Province. Two allelic family representative gene fragments were sequenced.


RESULTS
From 36 out of 39 blood samples from Plasmodium falciparum patients, 44 gene fragments of blocks 2 and 3 of the MSP1 were amplified, of which the MAD20-type allele was dominant(75%). followed by K1-type allele. No RO33-type allele was found. The mixed infection rate of the two different allelic type was 19.4%. Sequence analysis showed that the sequences of MAD20- and K1-type isolates from Hainan Province were highly homologous to that of the MAD20 and K1 allelic prototypes.


CONCLUSIONS
Two principal allelic types of MSP1 gene, MAD20- and K1-type, exist in malaria endemic areas in Hainan Province, the MAD20-type being the dominant.
Jiang
G F
GF
Department of Medical Parasitology, Guangdong College of Pharmacy, Guangzhou 510224.
Hong
J D
JD
Chen
P Q
PQ
Wang
S Q
SQ
Meng
F
F
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002681:China; D005784:Gene Amplification; D005838:Genotype; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology*; D020066:Merozoite Surface Protein 1 / Q000235:genetics*; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D020066:Merozoite Surface Protein 1