Seasonal population changes in the restriction fragment length polymorphism (RFLP) patterns from PCR-amplified 16S rRNA genes of predominant ribotypes in microbial mat samples from the Ebro Delta (Spain).
The stratified benthic microbial communities, which developed as a consequence of the physico-chemical gradients and the physiology of the inhabiting microorganisms, from Ebro Delta microbial mats were analyzed. 16S rRNA eubacterial and archaeal genes were amplified by polymerase chain reaction (PCR). PCR products were separately digested with three different restriction enzymes (AluI, HinfI, and RsaI) and later separated by horizontal electrophoresis to determine whether changes of predominant ribotypes are occurring over a period of a year. Comparison of total restriction patterns was performed by scoring similarities by using the Jaccard coefficient and then building a multidimensional scaling (MDS) map from the resulting similarities matrix. The three enzymes gave a consistent result, a seasonal distribution instead of a spatial and/or physiological one.
Ramírez-Moreno
Sergio
S
Genetics and Microbiology Department and Fundamental Biology Institute, Autonomous University of Barcelona, Campus de Bellaterra, E-08193 Bellaterra, Barcelona, Spain. sramirez@einstein-uab.es
Martínez-Alonso
María Ramos
MR
Méndez-Alvarez
Sebastián
S
Esteve
Isabel
I
Gaju
Núria
N
D001105:Archaea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018533:Biomass; D017753:Ecosystem; D019766:Genes, Archaeal; D005798:Genes, Bacterial; D010860:Pigments, Biological; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D021521:Ribotyping; D012621:Seasons; D013030:Spain
D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S