Hyperlayer hollow-fiber flow field-flow fractionation of cells.
Interest in low-cost, analytical-scale, highly efficient and sensitive separation methods for cells, among which bacteria, is increasing. Particle separation in hollow-fiber flow field-flow fractionation (HF FlFFF) has been recently improved by the optimization of the HF FIFFF channel design. The intrinsic simplicity and low cost of this HF FlFFF channel allows for its disposable usage. which is particularly appealing for analytical bio-applications. Here, for the first time, we present a feasibility study on high-performance, hyperlayer HF FIFFF of micrometer-sized bacteria (Escherichia coli) and of different types of cells (human red blood cells, wine-making yeast from Saccharomyces cerevisiae). Fractionation performance is shown to be at least comparable to that obtained with conventional, flat-channel hyperlayer FIFFF of cells, at superior size-based selectivity and reduced analysis time.
Reschiglian
Pierluigi
P
Department of Chemistry G. Ciamician, Via Selmi 2, 1-40126 Bologna, Italy. resky@ciam.unibo.it
Zattoni
Andrea
A
Roda
Barbara
B
Cinque
Leonardo
L
Melucci
Dora
D
Min
Byung Ryul
BR
Moon
Myeong Hee
MH
D004926:Escherichia coli; D031521:Fractionation, Field Flow / Q000379:methods*; D006801:Humans; D012441:Saccharomyces cerevisiae