[Analysis and structure identification of trace constituent in the total ginkgolide by using LC/DAD/ESI/MS].
OBJECTIVE
To identify the trace constituents in the total ginkgolide from leaves of Ginkgo biloba.


METHODS
The extract was analyzed by using LC/DAD/ESI/MS combination technology, both UV and mass spectrums of each compound were recorded simultaneously. UV, IR, MS and NMR spectra were employed for structural identification.


RESULTS
With higher sensitivity of the MS detector, three trace constituents were found as well as the known compounds ginkgolide A (GA), ginkgolide B (GB), ginkgolide C (GC) and bilobalide (BD).


CONCLUSIONS
Two compounds (tR 57.8 and 56.7 min) were identified as 1, 10-dihydroxy-3, 14-didehydroginkgolide and 10-hydroxy-3, 14-didehydroginkgolide, named as ginkgolide K and ginkgolide L, respectively, according to the characteristics of their UV, MS, IR and NMR spectra. It is the first time of finding the ginkgolide compounds containing double bond.
Wang
Y
Y
Analytical Chemistry Center, China Pharmaceutical University, Nanjing 210009, China. km4ywang@sina.com
Sheng
L S
LS
Lou
F C
FC
D002853:Chromatography, Liquid; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification*; D020441:Ginkgo biloba / Q000737:chemistry*; D046934:Ginkgolides; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; C481151:ginkgolide K; C481152:ginkgolide L