[Studies on the sensitivity of Plasmodium falciparum to pyronaridine/sulfadoxine/pyrimethamine in vitro].
OBJECTIVE
To develop an in vitro method for the assessment of drug response in P. falciparum to pyronaridine/sulfadoxine/pyrimethamine (PND/S/P).


METHODS
The PND/S/P microtest plate was designed, Rieckmann in vitro microtest(WHO standard kit) was used to test the sensitivity of P falciparum in continuous culture (FCC1/HN strain), and the data obtained were analyzed using a computer programme. Fractional inhibitory concentration (FIC) was calculated to test the possible synergy between PND and S/P.


RESULTS
The effect of the PND/S/P plates was fairly stable and the ED50 values of pyronaridine, sulfadoxine and pyrimethamine were 0.11, 215.12 and 2.9 pmol, respectively. The FIC obtained confirmed the synergism between PND and S/P.


CONCLUSIONS
The in vitro method can be used to assess the sensitivity of P. falciparum to pyronaridine/sulfadoxine/pyrimethamine.
Han
X L
XL
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Liu
D Q
DQ
Feng
X P
XP
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D004351:Drug Resistance; D004357:Drug Synergism; D009287:Naphthyridines / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000187:drug effects*; D011739:Pyrimethamine / Q000494:pharmacology; D013413:Sulfadoxine / Q000494:pharmacology
D000962:Antimalarials; D009287:Naphthyridines; D013413:Sulfadoxine; C027871:pyronaridine; D011739:Pyrimethamine