[Extended-spectrum beta-lactamase detection in Enterobacteriaceae and antibiotic susceptibility analysis].
OBJECTIVE
To detect the extended-spectrum beta-lactamases (ESBLs) in family Enterobacteriaceae and analyze the antibiotic susceptibility of those ESBLs-producing strains.


METHODS
ESBLs were determined by the double-disk confirmatory test and 8 antibiotic susceptibilities were tested with the disk disffusion method in those strains producing ESBLs.


RESULTS
Forty-seven ESBLs-producing strains comprised of 25 of E. coli, 14 of K. pneumoniae, 5 of E. cloacae, 1 of K. oxytoca, 1 of K. rhinoscleromatis, and 1 of S. liquefaciens. The susceptibility rates of those strains were: 100% for imipenem and meropenem, 89.4% for piperacillin/tazobactam, 72.4% for cefoxitin and 65.9% for cefotetan.


CONCLUSIONS
E. coli and K. pneumoniae are the prime strains producing ESBLs in Enterobacteriaceae. Imipenem and meropenem are the best drugs to deal with those ESBLs-producing strains. Piperacillin/tazobactam is better than cephamycins and other beta-lactama/beta-lactamase inhibitor combination.
Cao
Wei
W
Department of Clinical Laboratory, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Tong
Ming-hua
MH
Wang
Ji-gui
JG
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000201:enzymology*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology; D000077731:Meropenem; D008826:Microbial Sensitivity Tests; D010406:Penicillins / Q000494:pharmacology; D010878:Piperacillin / Q000494:pharmacology; D013845:Thienamycins / Q000494:pharmacology; D001618:beta-Lactamases / Q000032:analysis*
D000900:Anti-Bacterial Agents; D010406:Penicillins; D013845:Thienamycins; D015378:Imipenem; D001618:beta-Lactamases; D000077731:Meropenem; D010878:Piperacillin