[Effects of chlorine on the antigenicity and RNA polymorphism of hepatitis A virus].
The mechanisms of HAV inactivated by chlorine was investigated by enzyme-linked immunosorbent assay and RNA fingerprinting. Arbitrary primed PCR were involved to detect the changes of antigenicity and RNA polymorphism respectively before and after inactivation by chlorine. The results showed that the infectivity was completely inactivated at 10 mg/L or 20 mg/L of chlorine for 30 minutes and the contacting time should be prolonged to 60 minutes when the loss of antigenicity was observed at the same condition. The changes of RNA polymorphism were often observed while the infectivity was completely inactivated except the concentration of chlorine was 10 mg/L for 30 minutes. The results implied that HAV inactivation by chlorine might be the result of destruction of some fractions of HAV genome but not the whole HAV genome.
Xin
Zhongtao
Z
Institute of Hygiene and Environmental Medicine, Tianjin 300050, China.
Li
Junwen
J
Wang
Xinwei
X
Liu
Wei
W
D000956:Antigens, Viral / Q000276:immunology*; D002713:Chlorine / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D017957:Hepatitis A Virus, Human / Q000235:genetics* / Q000276:immunology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012367:RNA, Viral / Q000187:drug effects / Q000235:genetics*
D000956:Antigens, Viral; D012367:RNA, Viral; D002713:Chlorine