title: Determination of D-amino acid oxidase activity in tumour cells.
abstract: BACKGROUND
  We evaluated the assay for determining D-amino acid oxidase (DAAO) activity in tumour cells, rat liver and rat kidney for studying the effects of D-amino acid-containing solution on cancer patients.
  
  
  RESULTS
  In this method the amount of ammonia produced by the DAAO activity after removal of endogenous ammonia using a Sephadex G25 column was determined. The highest activity was observed in rat kidney, which was almost eight times that found in rat liver. As compared with host tissues, the DAAO activity in tumour cells was considerably less.
  
  
  CONCLUSIONS
  This DAAO assay may be useful for analysis of various tissue samples as well as tumour cells.
authors.affiliation: Pharmaceutical Research Laboratories, Ajinomoto Co Inc, 1-1 Suzuki-cho, Kawasaki-ku Kawasaki, 210-8681 Japan.
authors.forename: Taizo
authors.initials: T
authors.lastname: Sasamura
authors.forename: Akihiko
authors.initials: A
authors.lastname: Matsuda
authors.forename: Yukifumi
authors.initials: Y
authors.lastname: Kokuba
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000032:analysis* / Q000378:metabolism*; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000201:enzymology*; D051381:Rats; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D000641:Ammonia; D000594:Amino Acid Oxidoreductases