title: Sphingosylphosphorylcholine stimulates cellular fibronectin expression through upregulation of IL-6 in cultured human dermal fibroblasts.
abstract: Sphingosylphosphorylcholine (SPC) has been reported to stimulate wound healing by its potent mitogenic effect. Fibronectin (FN) is a cell-adhesion protein that plays an important role in cell migration and collagen deposition during wound healing. In order to elucidate further the mechanism involved in the accelerated wound healing stimulated by SPC, we studied the role of SPC in FN production in cultured human dermal fibroblasts. We demonstrated that SPC dose- and time-dependently enhanced the expression of FN in human dermal fibroblast at the protein and mRNA levels. IL-6 is known to stimulate the production of FN in fibroblasts. SPC also markedly induced IL-6 production in cultured human dermal fibroblasts in a dose- and time-dependent manner. We also demonstrated that FN mRNA expression in human dermal fibroblasts was upregulated 4 h after IL-6 treatment. Moreover, pretreatment with neutralizing anti-IL-6 antibodies partially blocked the upregulation of FN mRNA expression induced by SPC in human dermal fibroblasts. These results indicate that SPC may stimulate FN synthesis through IL-6 production in cultured human dermal fibroblasts.
authors.affiliation: Department of Dermatology, Chungnam National University School of Medicine, Daesa-dong 640, Jung-ku, Daejeon 301-040, Korea. seokb@cnu.ac.kr
authors.forename: Ki-Beom
authors.initials: KB
authors.lastname: Suhr
authors.forename: Ryoji
authors.initials: R
authors.lastname: Tsuboi
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Seo
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Piao
authors.forename: Jeung-Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Jang-Kyu
authors.initials: JK
authors.lastname: Park
authors.forename: Hideoki
authors.initials: H
authors.lastname: Ogawa
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000276:immunology / Q000378:metabolism*; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000378:metabolism; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D000906:Antibodies; D005353:Fibronectins; D015850:Interleukin-6; D012333:RNA, Messenger; C005356:sphingosine phosphorylcholine; D010767:Phosphorylcholine; D013110:Sphingosine