[Effect of bushen huoxue decoction on in vitro fertilization and early embryonic development in mice].
OBJECTIVE
To explore the effect of Bushen Huoxue decoction (BSHX) on female reproduction and elucidate its therapeutic mechanism to infertility.


METHODS
The BSHX medicated serum of rabbit, as a supplement, was co-cultured with the sperm and ovum of non-copulated mice, and the 2-cell embryos of copulated female mice separately, to observe the changes of in vitro fertilization rate (IVF) and early embryogenesis rate.


RESULTS
By co-cultured with BSHX medicated serum, IVF rate was increased obviously (P < 0.01), and the follow-up early embryogenesis rate at various period was promoted, particularly that of the 4-cell and 8-cell embryos (P < 0.05). No influence on developmental rate of in vitro 4-cell embryos obtained from the in vivo 2-cell embryos. But the development of the 8-cell embryos, morula, blastula and hatching were promoted significantly.


CONCLUSIONS
BSHX could raise the fertilization rate and promote the early embryonic development.
Yang
G Y
GY
Jiangsu Provincial Hospital of Integrated Traditional and Western Medicine, Nanjing 210028.
Wang
P J
PJ
Jia
X B
XB
D000818:Animals; D018920:Coculture Techniques; D046508:Culture Techniques; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects*; D006801:Humans; D008297:Male; D051379:Mice; D010063:Ovum / Q000166:cytology; D011817:Rabbits; D013094:Spermatozoa / Q000166:cytology
D004365:Drugs, Chinese Herbal