title: [Studies on the sensitivity of Plasmodium falciparum to pyronaridine/sulfadoxine/pyrimethamine in vitro].
abstract: OBJECTIVE
  To develop an in vitro method for the assessment of drug response in P. falciparum to pyronaridine/sulfadoxine/pyrimethamine (PND/S/P).
  
  
  METHODS
  The PND/S/P microtest plate was designed, Rieckmann in vitro microtest(WHO standard kit) was used to test the sensitivity of P falciparum in continuous culture (FCC1/HN strain), and the data obtained were analyzed using a computer programme. Fractional inhibitory concentration (FIC) was calculated to test the possible synergy between PND and S/P.
  
  
  RESULTS
  The effect of the PND/S/P plates was fairly stable and the ED50 values of pyronaridine, sulfadoxine and pyrimethamine were 0.11, 215.12 and 2.9 pmol, respectively. The FIC obtained confirmed the synergism between PND and S/P.
  
  
  CONCLUSIONS
  The in vitro method can be used to assess the sensitivity of P. falciparum to pyronaridine/sulfadoxine/pyrimethamine.
authors.affiliation: Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
authors.forename: X L
authors.initials: XL
authors.lastname: Han
authors.forename: D Q
authors.initials: DQ
authors.lastname: Liu
authors.forename: X P
authors.initials: XP
authors.lastname: Feng
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D004351:Drug Resistance; D004357:Drug Synergism; D009287:Naphthyridines / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000187:drug effects*; D011739:Pyrimethamine / Q000494:pharmacology; D013413:Sulfadoxine / Q000494:pharmacology
chemical_list: D000962:Antimalarials; D009287:Naphthyridines; D013413:Sulfadoxine; C027871:pyronaridine; D011739:Pyrimethamine