Simple and rapid determination of thiabendazole, imazalil, and o-phenylphenol in citrus fruit using flow-injection electrospray ionization tandem mass spectrometry.
A simple and rapid analytical method for thiabendazole (TBZ), imazalil (IMA), and o-phenylphenol (OPP) in citrus fruit has been developed by using flow-injection electrospray ionization tandem mass spectrometry for the first time. The method involves the combined use of stable isotopically labeled internal standards (thiabendazole-(13)C(6), imazalil-d(5), and p-phenylphenol-d(9)) and a multiple reaction monitoring technique. The average recoveries for the fungicides at the tolerance levels (TBZ and OPP, 10 mg/kg; IMA, 5 mg/kg) ranged from 77 to 101%, with the coefficients of variation (CVs) ranging from 0.7 to 4.2% (n = 5). At half the tolerance levels (TBZ and OPP, 5 mg/kg; IMA, 2.5 mg/kg), the average recoveries ranged from 62 to 112%, with the CVs ranging from 0.7 to 8.4% (n = 5). The CVs of the average recoveries, obtained from lemon samples fortified with three fungicides at the tolerance levels, obtained on three different days over two weeks, ranged within 2%. The analysis time, including sample preparation and determination, is only 15 min.
Ito
Yuko
Y
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan. yuuko_1_itou@pref.aichi.lg.jp
Goto
Tomomi
T
Oka
Hisao
H
Matsumoto
Hiroshi
H
Miyazaki
Yutaka
Y
Takahashi
Nobuyuki
N
Nakazawa
Hiroyuki
H
D001713:Biphenyl Compounds / Q000032:analysis; D002957:Citrus / Q000737:chemistry*; D017022:Flow Injection Analysis; D005638:Fruit / Q000737:chemistry*; D005659:Fungicides, Industrial / Q000032:analysis*; D007093:Imidazoles / Q000032:analysis; D011786:Quality Control; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000592:standards; D013827:Thiabendazole / Q000032:analysis
D001713:Biphenyl Compounds; D005659:Fungicides, Industrial; D007093:Imidazoles; C017435:enilconazole; C004369:2-phenylphenol; D013827:Thiabendazole