[The preliminary study of amniotic membrane on TGF-beta 1 expression after photorefractive keratectomy].
OBJECTIVE
To investigate the effect of amniotic membrane on TGF-beta 1 expression after photorefractive keratectomy(PRK).


METHODS
PRK was performed bilaterally on 6 New Zealand white rabbits. One eye was randomly tranplanted with preserved human amniotic membrane, and the other eye served as the control. The expression of TGF-beta 1 mRNA was detected with in situ hybridization at the 4th weeks after PRK.


RESULTS
TGF-beta 1 mRNA expression was present in the corneal epithelium and stroma at the 4th weeks after PRK. The cornea in amniotic membrane transplanted group had also TGF-beta 1 mRNA expression, and the expression extent was less than that in control group (P < 0.05).


CONCLUSIONS
Amniotic membrane can reduce the expression of TGF-beta 1 mRNA in cornea after PRK.
Zhong
Y
Y
Ophthalmic Center, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Zhou
Y
Y
Wang
K
K
D000650:Amnion / Q000637:transplantation*; D000818:Animals; D003315:Cornea / Q000378:metabolism* / Q000601:surgery; D003319:Corneal Stroma / Q000378:metabolism; D019573:Epithelium, Corneal / Q000378:metabolism; D005260:Female; D006801:Humans; D054018:Lasers, Excimer; D008297:Male; D018903:Photorefractive Keratectomy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011817:Rabbits; D011897:Random Allocation; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D053773:Transforming Growth Factor beta1
D012333:RNA, Messenger; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1