title: Mutations in sit B and sit D genes affect manganese-growth requirements in Sinorhizobium meliloti.
abstract: Two transposon-induced mutants of Sinorhizobium meliloti 242 were isolated based on their inability to grow on rich medium supplemented with the metal chelator ethylenediamine di-o-hydroxyphenylacetic acid (EDDHA) and either heme-compounds or siderophores as iron sources. Tagged loci of these mutants were identified as sit B and sit D genes. These genes encode components of an ABC (ATP-binding cassette) metal-type permease in several Gram-negative bacteria. In this work, the phenotypes of these two mutants were compared with those of two siderophore-mediated iron transport mutants. The results strongly implicate a role of the sit genes in manganese acquisition when this metal is limiting in S. meliloti.
authors.affiliation: Departamento de Bioquímica, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Av Italia 3318, Montevideo 11600, Uruguay.
authors.forename: Raúl A
authors.initials: RA
authors.lastname: Platero
authors.forename: Melina
authors.initials: M
authors.lastname: Jaureguy
authors.forename: Federico J
authors.initials: FJ
authors.lastname: Battistoni
authors.forename: Elena R
authors.initials: ER
authors.lastname: Fabiano
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D004251:DNA Transposable Elements; D005029:Ethylenediamines / Q000494:pharmacology; D005798:Genes, Bacterial / Q000235:genetics; D007501:Iron / Q000378:metabolism; D007502:Iron Chelating Agents / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology*; D000455:Medicago sativa / Q000254:growth & development / Q000382:microbiology; D009154:Mutation; D017422:Sequence Analysis, DNA; D017262:Siderophores / Q000235:genetics / Q000378:metabolism; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D013559:Symbiosis
chemical_list: D018528:ATP-Binding Cassette Transporters; D004251:DNA Transposable Elements; D005029:Ethylenediamines; D007502:Iron Chelating Agents; D017262:Siderophores; C011813:ethylenediamine-N,N'-bis(2-hydroxyphenylacetic acid); D008345:Manganese; D007501:Iron