Identification of microsatellites in expressed muscle genes: assessment of a desmin (CT) dinucleotide repeat as a marker for meat quality.
We investigated the presence of dinucleotide repeats (microsatellites) in a porcine skeletal muscle cDNA library. In total, microsatellites were found in nearly 4% of all muscle cDNA clones, of which CT- and TG-repeats were the most prevalent. We found a near full-length desmin cDNA clone with a CT-repeat sited downstream from its stop codon. From 44 commercially reared pigs, six allelic length polymorphisms were identified at this microsatellite locus (128, 131, 134, 135, 136, and 138 bp), whose desmin genotypes were associated with pH change after slaughter (P=0.031) and colour variation of meat (P<0.001 for hue, P<0.005 for lightness, and P=0.014 for b colour). Two of the genotypes (131/131 bp and 131/135 bp) were consistent with paler meat. This finding indicates the usefulness of the cDNA library approach to generate markers for marker-assisted selection and suggests that the desmin microsatellite could be a possible selection marker.
Chang
K C
KC
Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Glasgow, Glasgow G61 1QH, UK. k.chang@vet.gla.ac.uk
Beuzen
N D
ND
Hall
A D
AD
D000818:Animals; D001483:Base Sequence; D003116:Color; D018076:DNA, Complementary / Q000235:genetics; D018900:Dinucleotide Repeats / Q000235:genetics*; D005819:Genetic Markers; D005838:Genotype; D008460:Meat / Q000592:standards*; D008969:Molecular Sequence Data; D011786:Quality Control; D013552:Swine
D018076:DNA, Complementary; D005819:Genetic Markers