title: Beta 3-adrenoceptor knockout in C57BL/6J mice depresses the occurrence of brown adipocytes in white fat.
abstract: White and brown adipocytes are usually located in distinct depots; however, in response to cold, brown adipocytes appear in white fat. This response is mediated by beta-adrenoceptors but there is a controversy about the subtype(s) involved. In the present study, we exposed to cold beta 3-adrenoceptor knockout mice (beta 3KO) on a C57BL/6J genetic background and measured in white adipose tissue the density of multilocular cells and the expression of the brown adipocyte marker uncoupling protein-1 (UCP1). In brown fat of beta 3KO mice, UCP1 expression levels were normal at 24 degrees C as well as after a 10-day cold exposure. Strikingly, under both conditions, in the white fat of beta 3KO mice the levels of UCP1 mRNA and protein as well as the density of multilocular cells were decreased. These results indicate that beta 3-adrenoceptors play a major role in the appearance of brown adipocytes in white fat and suggest that the brown adipocytes present in white fat differ from those in brown fat.
authors.affiliation: Département de Biochimie Médicaleet Physiologie, Centre Médical Universitaire, Geneva, Switzerland. Maria.Jimenez@medecine.unige.ch
authors.forename: Maria
authors.initials: M
authors.lastname: Jimenez
authors.forename: Giorgio
authors.initials: G
authors.lastname: Barbatelli
authors.forename: Roberta
authors.initials: R
authors.lastname: Allevi
authors.forename: Saverio
authors.initials: S
authors.lastname: Cinti
authors.forename: Josiane
authors.initials: J
authors.lastname: Seydoux
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Giacobino
authors.forename: Patrick
authors.initials: P
authors.lastname: Muzzin
authors.forename: Frédéric
authors.initials: F
authors.lastname: Preitner
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D007124:Immunoenzyme Techniques; D007473:Ion Channels; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins; D012333:RNA, Messenger / Q000378:metabolism; D022702:Receptors, Adrenergic, beta-3 / Q000502:physiology*; D014475:Uncoupling Agents / Q000378:metabolism*; D000071256:Uncoupling Protein 1
chemical_list: D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D022702:Receptors, Adrenergic, beta-3; C000604372:Ucp1 protein, mouse; D014475:Uncoupling Agents; D000071256:Uncoupling Protein 1; D003576:Electron Transport Complex IV