Effect of oxidative stress on serum and antioxidant enzymes in liver and kidney of rats and their modulation through dietary factors.
Modulatory effect of a formulated diet based on cereals, pulses and spices incorporated with crude palm oil (CPO), soybean oil (SBO) or cod liver oil (CLO) at 10% dietary level on oxidative stress and antioxidant enzymes was studied in liver and kidney tissues. Activity of alkaline phosphatase (ALP) and acid phosphatase (ACP) increased significantly in serum in various experimental groups. Significant increase in hepatic antioxidant enzymes, catalase, glutathione peroxidase (GPx) was also seen in the experimental groups. SOD activity showed a mixed response. Further, kidney antioxidant enzymes did not show much change compared to those in liver. The results indicated dietary lipid as the key players in determining cellular susceptibility to oxidative stress, which could be modulated by cereals, pulses and spices in the diet.
Ramachandran
H D
HD
Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore 570 013, India.
Narasimhamurthy
K
K
Raina
P L
PL
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002374:Catalase / Q000378:metabolism; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
D004041:Dietary Fats; D010088:Oxidoreductases; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase