In vitro antimalarial effect of daphnetin relating to its iron-chelating activity.
OBJECTIVE
To investigate the in vitro antimalarial effect of daphentin relating to its iron-chelating activity.


METHODS
Schizontocidal activity of daphnetin and desferrioxamine B was tested through an in vitro assay based on the routine in vitro cultivation of Plasmodium faciparum FCC1 strain. The iron-chelating ability of each was measured by the fluorescent probe calcein.


RESULTS
Daphnetin exhibited a modest iron-chelating ability compared with the powerful iron-chelator desferrioxamine B. In vitro test at the range of 0-12 mumol/L daphnetin showed a dose-dependent schizontocidal activity which could be inhibited if mixed with Fe2+ in a ratio of 2:1.


CONCLUSIONS
The dose-dependent antimalarial activity of daphnetin is related to its iron-chelating activity.
Mu
Ling-yun
LY
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
Wang
Qin-mei
QM
Ni
Yi-chang
YC
D000818:Animals; D000962:Antimalarials / Q000378:metabolism / Q000494:pharmacology*; D003676:Deferoxamine / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007502:Iron Chelating Agents / Q000378:metabolism / Q000494:pharmacology*; D010963:Plasmodium falciparum / Q000187:drug effects*; D014468:Umbelliferones / Q000378:metabolism / Q000494:pharmacology*
D000962:Antimalarials; D007502:Iron Chelating Agents; D014468:Umbelliferones; D003676:Deferoxamine; C039952:daphnetin