[C-fos gene expression in the rat spinal cord and brain cells during stress and the use of different types of halothane anesthesia].
C-fos gene expression was studied as a marker of nervous cells activation in the rat spinal cord and brain cells under different conditions (different kinds of narcosis). Using of 1.5% light halothane narcosis allowed the detection of c-Fos-like proteins expression in the spinal cord cells only. Under initial 1.5% halothane narcosis, c-Fos-like proteins expression in the rat spinal cord (lumbar segments) and the brain cells was observed after placing the rats into the hammock, noxious mechanical stimulation (NMS) or high frequency electromagnetic irradiation of the skin (EHF). The pattern of the brain structures reacting on the NMS by c-Fos proteins expression, was determined. It was shown that NMS increases the c-Fos positive cell quantity in the lateral hypothalamic area (LHA), ventro-medial (VMH), dorso-medial (DMH) hypothalamic nuclei and anterior hypothalamic area (AHN) by 116, 199, 101 and 157% resp., in comparison with the c-Fos immunoreactive cell quantity in intact animals. EHF irradiation of the skin decreased the intensity of c-Fos-like proteins synthesis induced by NMS in the most of the investigated structures (LHA, VMH, DMH and AHA by 32.8, 29, 15 and 33%, resp.). It was shown that only initial halothane narcosis allowed to determine the hypothalamic structures which realized the responses to the NMS, and modification effects of EHF skin irradiation on the intensity of these responses.
Novikova
N S
NS
Institute of Experimental Medicine of the Russian Acad. Med. Sci., St. Petersburg, 197376, Acad. Pavlov St., 12, Russia.
Kazakova
T B
TB
Rogers
V
V
Korneva
E A
EA
D000769:Anesthesia, Inhalation; D018685:Anesthetics, Inhalation / Q000494:pharmacology; D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D016762:Genes, fos; D006221:Halothane / Q000494:pharmacology; D007031:Hypothalamus / Q000166:cytology / Q000378:metabolism; D008297:Male; D008872:Microwaves; D010812:Physical Stimulation; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics / Q000378:metabolism*
D018685:Anesthetics, Inhalation; D016760:Proto-Oncogene Proteins c-fos; D006221:Halothane