Influence of the platelet-activating factor receptor antagonist BB-882 on intra-abdominal adhesion formation in rats.
Postoperative intra-abdominal adhesion formation is a major clinical problem. We aimed to examine the preventive effect of treatment with the platelet-activating factor (PAF) antagonist (lexipafant, BB-882) on experimentally induced intra-abdominal adhesion formation in rats. Twenty male Sprague-Dawley rats weighing 250 and 290 g were studied. Generation of adhesions in rats by brushing a 1-cm(2) area of the cecum and the peritoneum on the right side of the abdominal wall was followed by intra-abdominal administration of saline and 5 mg/kg in a volume of 0.2 ml PAF receptor antagonist BB-882. After 45 days, formation of adhesions was graded and histological evaluation was processed. The severity of adhesions was significantly less in the BB-882 group than in the control group (p < 0.001, p < 0.05). The average adhesion scores in the control and BB-882 groups were 3.2 +/- 0.6 and 0.6 +/- 0.6, respectively, and the difference between both groups was found to be significant (p < 0.0001). The number of polymorphonuclear leukocytes and fibrotic areas was significantly decreased in the BB-882 group when compared to the control group (p < 0.001, p < 0.002). In conclusion, this study confirms the efficacy of BB-882 in the prevention of postoperative intra-abdominal adhesions in a rat model.
Otcu
S
S
Department of Pediatric Surgery, Dicle University Medical School, Diyarbakir, Turkey. selcukotcu@hotmail.com
Ozturk
H
H
Aldemir
M
M
Kilinc
N
N
Dokucu
A I
AI
D000005:Abdomen / Q000473:pathology; D000818:Animals; D005355:Fibrosis; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D007958:Leukocyte Count; D008297:Male; D009504:Neutrophils / Q000473:pathology; D010980:Platelet Membrane Glycoproteins / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors*; D043562:Receptors, G-Protein-Coupled; D012720:Severity of Illness Index; D000267:Tissue Adhesions / Q000473:pathology / Q000517:prevention & control*
C094498:BB 882; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor; D007930:Leucine