title: Molecular cloning, structure, and testis-specific expression of MFSJ1, a member of the DNAJ protein family, in the Japanese monkey (Macaca fuscata).
abstract: A strong signal of cDNA product was identified in adult and senile testes of the Japanese monkeys (Macaca fuscata) using differential display PCR analysis. Its full-length cDNA was molecular-cloned by RT-PCR using adult testis mRNA as templates. The predicted open reading frame encoded a protein of 242 amino-acid residues. It contained J domain in the NH(2) terminal region and Gly/Phe-rich domain in the middle of protein, which are typical structural domains of the DnaJ protein family. We named this gene, MFSJ1, for spermatogenic cell-specific DNAJ homolog in the Japanese monkey. Northern blot analysis of RNAs from various somatic and germinal tissues revealed that the MFSJ1 gene is specifically expressed in testis and is active at adult and senile stages but is scarcely expressed at the juvenile stage. In situ hybridization revealed that the MFSJ1 gene is expressed mainly in spermatids and the expressional potential is maintained from adult to senile stages. MFSJ1 was found to have high similarity (71% identity) with MSJ1, mouse spermatogenic cell-specific DnaJ homolog. Although this type of DnaJ-like protein has not been found in other mammals, it may be essential for mammalian spermatogenesis.
authors.affiliation: Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
authors.forename: Sung Sook
authors.initials: SS
authors.lastname: Yu
authors.forename: Osamu
authors.initials: O
authors.lastname: Takenaka
mesh_terms: D000375:Aging / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D008251:Macaca / Q000033:anatomy & histology / Q000502:physiology*; D008297:Male; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013737:Testis / Q000166:cytology / Q000502:physiology*
chemical_list: D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger