title: [Detection of anti-Spirometra erinaceieuropaei antibody using ELISA].
abstract: OBJECTIVE
  To study specific diagnosis of Spirometra erinaceieuropaei.
  
  
  METHODS
  An enzyme-linked immunosorbent assay (ELISA) was studied using highly pure gene engineering antigen expressed by the recombination of the cloned cysteine proteinase gene of Spirametra erinaceieuropaei with expression vector pMAL-c2. Six sera from patient infected with Spirometra erinaceieuropaei were detected using this method.
  
  
  CONCLUSIONS
  The results showed that the gene engineering antigen reacted strongly with the sera from Spirometra erinaceieuropaei-infected patients, but did not with the sera from Cysticercus cellulosae-infected patients.
authors.affiliation: Department of Epidemiology, Hebei Medical University, Shijiazhuang 050017.
authors.forename: Y X
authors.initials: YX
authors.lastname: Ding
authors.forename: L L
authors.initials: LL
authors.lastname: Guo
authors.forename: D W
authors.initials: DW
authors.lastname: Liu
authors.forename: P H
authors.initials: PH
authors.lastname: Zhang
authors.forename: S X
authors.initials: SX
authors.lastname: Liu
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology; D002590:Cestode Infections / Q000175:diagnosis* / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D012680:Sensitivity and Specificity; D013146:Spirometra / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth