title: [Genetic polymorphism of six STR loci in the Han population in Henan province].
abstract: To investigate the allele frequencies of six short tandem repeats (STR) loci: F13A1, F13B, D8S1179, CSF1PO, D5S818 and TPOX in Han population in Henan province, DNA was extracted with chelex from EDTA-blood samples of the unrelated individuals in Henan province and amplified with PCR technique. The PCR product was analyzed with the undenatured PAGE vertical electrophoresis and silverstain. The results were obtained through genetical analysis. The authors got the number of allele of six loci, Three alleles of F13A1 could be detected in this population. The F13A1 * 7 is the most common allele with a frequency of 45.2%, followed by F13A1 * 5 and F13A1 * 4 respectively. F13B had four alleles among which F13B * 10 has the highest frequency, followed by F13B * 9, F13B * 8, F13B * 11. The 8 alleles of D8S1179 in the order from high to low frequency is D8S1179 * 14, * 15, * 12, * 11, * 10. And the most common frequencies of alleles of CSF1PO, D5S818, TPOX ore CSF1PO * 12, D5S818 * 11, TPOX * 8, respectively followed by the allele of * 11, * 14, * 10, * 13 in CSF1PO locus, and * 12, * 13, * 10, * 9, * 14, * 8, * 15, * 7 in D5S818 locus, * 11, * 9, * 12, * 10 in TPOX locus. The heterozygosities of the six loci were 0.62, 0.46, 0.83, 0.59, 0.78 and 0.65; the discrimination powers were 0.78, 0.66, 0.95, 0.79, 0.92 and 0.82. The heterozygosities of the six loci are high and the frequencies are in good agreement with Hardy-Weinberg equilibrium. The six loci are good as genetic markers. We can use these loci in dividualidentification and partenity test in forensic area.
authors.affiliation: Medical Genetic Institute of Henan Province, People's Hospital of Henan Province, Zhengzhou, 450003, China.
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Si
authors.forename: Bing-Tao
authors.initials: BT
authors.lastname: Hao
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Ying-Tai
authors.initials: YT
authors.lastname: Wang
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Zhu
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Yang
authors.forename: Shi-Xiu
authors.initials: SX
authors.lastname: Liao
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Huang
authors.forename: Zhao-Cai
authors.initials: ZC
authors.lastname: Wang
mesh_terms: D000483:Alleles; D002681:China; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences / Q000235:genetics*
chemical_list: D005819:Genetic Markers