Impaired angiogenesis in neuropeptide Y (NPY)-Y2 receptor knockout mice.
Which of Y1-Y5 receptors (Rs) mediate NPY's angiogenic activity was studied using Y2R-null mice and R-specific antagonists. In Y2R-null mice, NPY-induced aortic sprouting and in vivo Matrigel capillary formation were decreased by 50%; Y1R-antagonist blocked the remaining response. NPY-induced sprouting was equally inhibited by Y2R- (and Y5R- but less by Y1R-) antagonists in wild type mice. Spontaneous and NPY-induced revascularization of ischemic gastrocnemius muscles were similarly reduced in Y2R-null mice. Thus, NPY-induced angiogenesis, spontaneous and ischemic, is primarily mediated by Y2Rs. However, Y5Rs and, to a lesser degree Y1Rs, also may play a role in NPY-mediated angiogenesis.
Lee
Edward W
EW
Department of Physiology & Biophysics, Georgetown University Medical Center, 3900 Reservoir Road, NW, BSB 234, Washington, DC 20007, USA. leeew@georgetown.edu
Grant
Derrick S
DS
Movafagh
Sharareh
S
Zukowska
Zofia
Z
D000818:Animals; D002196:Capillaries / Q000254:growth & development; D051379:Mice; D018345:Mice, Knockout; D018919:Neovascularization, Physiologic / Q000502:physiology*; D017476:Receptors, Neuropeptide Y / Q000235:genetics / Q000502:physiology*
D017476:Receptors, Neuropeptide Y; C095889:neuropeptide Y2 receptor