Gene expression of cell surface antigens in the early phase of murine influenza pneumonia determined by a cDNA expression array technique.
BACKGROUND
Influenza virus is a worldwide health problem with significant economic consequences. To study the gene expression pattern induced by influenza virus infection, it is useful to reveal the pathogenesis of influenza virus infection; but this has not been well examined, especially in vivo study.


OBJECTIVE
To assess the influence of influenza virus infection on gene expression in mice, mRNA levels in the lung and tracheal tissue 48 h after infection were investigated by cDNA array analysis.


METHODS
Four-week-old outbred, specific pathogen free strain, ICR female mice were infected by intra-nasal inoculation of a virus solution under ether anesthesia. The mice were sacrificed 48 h after infection and the tracheas and lungs were removed. To determine gene expression, the membrane-based microtechnique with an Atlas cDNA expression array (mouse 1.2 array II) was performed in accordance with the manual provided.


CONCLUSIONS
We focused on the expression of 46 mRNAs for cell surface antigens. Of these 46 mRNAs that we examined, four (CD1d2 antigen, CD39 antigen-like 1, CD39 antigen-like 3, CD68 antigen) were up-regulated and one (CD36 antigen) was down-regulated. Although further studies are required, these data suggest that these molecules play an important role in influenza virus infection, especially the phase before specific immunity.
Sakai
Shinya
S
Department of Japanese Oriental (Kampo) Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan. shinyas@ms.toyama-mpu.ac.jp
Mantani
Naoki
N
Kogure
Toshiaki
T
Ochiai
Hiroshi
H
Shimada
Yutaka
Y
Terasawa
Katsutoshi
K
D000251:Adenosine Triphosphatases / Q000235:genetics; D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D018949:Antigens, CD1 / Q000235:genetics; D055610:Antigens, CD1d; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics; D000954:Antigens, Surface / Q000235:genetics*; D001081:Apyrase; D002642:Chick Embryo; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D051379:Mice; D008813:Mice, Inbred ICR; D020411:Oligonucleotide Array Sequence Analysis; D009976:Orthomyxoviridae Infections; D011014:Pneumonia / Q000276:immunology* / Q000382:microbiology*; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation; D000097382:CD68 Molecule
D000251:Adenosine Triphosphatases; D015703:Antigens, CD; D018949:Antigens, CD1; D055610:Antigens, CD1d; D015214:Antigens, Differentiation, Myelomonocytic; D000954:Antigens, Surface; D001081:Apyrase; D012333:RNA, Messenger; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C067869:CD39 antigen