[Study on sense neurotrophism of denervated red and white muscles].
OBJECTIVE
To clarify the character of nerve growth factor (NGF) in the denervated red and white muscles and the relationship between the amount of NGF and sensitive neurotrophism of denervated red and white muscles.


METHODS
The model of the denervated gastrocnemius and soleus was made by clipping the sciatic nerve of Wister rats. The immunohistochemistry was taken to measure the amount of NGF in muscles, and the neurotrophism of extracts of muscles was tested with culture of dorsal root ganglions at the 1st, 3rd, 7th and 14th days after injury.


RESULTS
The amount of NGF in denervated gastrocnemius and soleus decreased, especially in soleus. The neruotrophism of the extracts of the two kinds of denervated muscles did not decrease; on the contrary, it increased after a week after injury.


CONCLUSIONS
The injury of peripheral nerves causes the amount of NGF in the target tissues to increase, but the change is different between the denervated muscles; the neurotrophism of the extracts of musclesis determined by all kinds of neurotrophic factors, and can not be explained by a single factor.
Wang
Jing-bo
JB
Emergency and Trauma Department, Tianjin Orthopedics Hospital, Tianjin, P. R. China 300211.
Luo
Yong-xiang
YX
Liu
Wen-ge
WG
D000818:Animals; D046508:Culture Techniques; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009121:Muscle Denervation; D018482:Muscle, Skeletal / Q000294:innervation* / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000293:injuries*
D009414:Nerve Growth Factors