[A new method for detecting serum beta-lipoprotein electrophoretogram].
OBJECTIVE
To establish a new method for isolating serum beta-lipoprotein (betaLp) subfractions using second double gradient polyacrylamide gel electrophoresis (s-DGPAGE).


METHODS
After the betaLp subfractions were isolated by mechanisms of molecular sieving and isoelectric focusing due to density and pH grade in the non-linear density gradient polyacrylamide gel, the gels were acquired with a gel imaging system, the electrophoretograms were quantitatively analyzed with optical density scanner. The isolation of serum betaLp was completed through first DGPAGE.


RESULTS
s-DGPAGE separated the serum betaLp into four subfractions, designated respectively as betaLp1, betaLp2, betaLp3 and betaLp4, and their amounts in the betaLp and in the whole lipoproteins could be quantified.


CONCLUSIONS
s-DGPAGE is a simple and practical method to show the heterogeneity of serum betaLp and the quantitative variation of its subfractions.
Chen
Yun-qin
YQ
Electrocardiology Research Center, Department of Basic Medicine, First Military Medical University, Guangzhou 510515, China. SLPG@fimmu.edu.cn
Yin
Jia
J
Zhang
Min
M
Yin
Bing-sheng
BS
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D008077:Lipoproteins, LDL / Q000032:analysis / Q000097:blood*; D015203:Reproducibility of Results
D008077:Lipoproteins, LDL