Postnatal intracerebroventricular exposure to neuropeptide Y causes weight loss in female adult rats.
We investigated the effect of repetitive postnatal (2-7 days) intracerebroventricular administration of neuropeptide Y (NPY) on food intake and body weight gain in the 3- to 120-day-old Sprague-Dawley rats. NPY caused a 32% transient increase in body weight gain with elevated circulating insulin concentrations within 24 h. This early intervention led to the persistence of hyperinsulinemia and relative hyperleptinemia with euglycemia in the 120-day-old female alone. This perturbation was associated with 50% suppression in adult female hypothalamic NPY concentrations and a 50-85% decline in NPY immunoreactivity in the paraventricular and arcuate nuclei. This change was paralleled by a approximately 20% decline in food intake and body weight gain at 60 and 120 days. However, when exogenous NPY was stereotaxically reinjected into the paraventricular nucleus of the approximately 120-day-old adult females who were pretreated with NPY postnatally, an increase in food intake and body weight gain was noted, attesting to no disruption in the NPY end-organ responsivity. We conclude that postnatal intracerebroventricular NPY has long-lasting effects that predetermine the resultant adult phenotype in a sex-specific manner.
Varma
Amit
A
University of Pittsburgh Schools of Medicine; Public Health, Pittsburgh, Pennsylvania 15213-3180, USA.
He
Jing
J
Weissfeld
Lisa
L
Devaskar
Sherin U
SU
D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D001786:Blood Glucose / Q000032:analysis; D005247:Feeding Behavior / Q000187:drug effects; D005260:Female; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009478:Neuropeptide Y / Q000008:administration & dosage* / Q000494:pharmacology*; D009929:Organ Size; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012727:Sex Characteristics; D013997:Time Factors; D015431:Weight Loss / Q000187:drug effects* / Q000502:physiology
D001786:Blood Glucose; D007328:Insulin; D020738:Leptin; D009478:Neuropeptide Y; D007986:Luteinizing Hormone