Construction of a protein-detection system using a loop peptide library with a fluorescence label.
Construction of a novel protein-detection system was carried out using a designed peptide library with fluorescent labels based on loop structures. As a basic model study, detection of alpha-amylase using fluorescent-labeled peptides derived from an active loop of tendamistat was examined. The detection methods for proteins with immobilized peptides as well as peptides in solution have been successfully established. Based on these results, a loop peptide library that has various turn sequences grafted on a stable loop structure has been constructed. Various proteins with recognition patterns corresponding, for instance, to "protein fingerprints" could be detected using an immobilized peptide library. The present results suggest that the system can be applied to the development of a peptide microarray that behaves as a protein chip.
Takahashi
Mizuki
M
Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
Nokihara
Kiyoshi
K
Mihara
Hisakazu
H
D000595:Amino Acid Sequence; D001665:Binding Sites; D004791:Enzyme Inhibitors / Q000737:chemistry; D005456:Fluorescent Dyes; D008722:Methods; D010446:Peptide Fragments / Q000737:chemistry; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000737:chemistry; D000516:alpha-Amylases / Q000032:analysis / Q000037:antagonists & inhibitors / Q000737:chemistry
D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D019151:Peptide Library; D010455:Peptides; D011506:Proteins; D000516:alpha-Amylases; C030526:tendamistate