title: Activity of matrix metalloproteinases-2 and -9 in advanced laryngeal cancer.
abstract: OBJECTIVE
  Matrix metalloproteinases-2 and -9 (MMP-2 and MMP-9) are proteolytic enzymes that digest collagen type IV and other components of the basement membrane. They play a key role in local invasion and the formation of distant metastases by malignant tumors. The aim of this study was to evaluate the activity of MMP-2 and MMP-9 in stage III and IV laryngeal cancers.
  
  
  METHODS
  In the study we used specimens of laryngeal cancer and surrounding normal mucosa obtained from 23 patients undergoing surgical treatment as a primary therapy. After extraction of MMP-2 and MMP-9 from tissue samples, their activity was assessed with zymography.
  
  
  RESULTS
  Greater activity of MMP-2 and MMP-9 and a higher active/latent MMP-2 ratio were found in cancer compared with normal mucosa. Moreover, N2 tumors revealed greater activity of MMP-2 in comparison with N1 and N0 tumors.
  
  
  CONCLUSIONS
  Results of the study indicate that both MMP-2 and MMP-9 may be involved in the expansion of laryngeal cancer. MMP-2 may also play an important role in the lymphatic spread of some laryngeal tumors.
authors.affiliation: Department of Biochemistry and Molecular Biology and Otolaryngology, University School of Medicine, Lublin, Poland. mbogusiewics@yahoo.com
authors.forename: Michal
authors.initials: M
authors.lastname: Bogusiewicz
authors.forename: Marta
authors.initials: M
authors.lastname: Stryjecka-Zimmer
authors.forename: Marcin
authors.initials: M
authors.lastname: Szymanski
authors.forename: Tomasz
authors.initials: T
authors.lastname: Rechberger
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Golabek
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D001707:Biopsy, Needle; D016022:Case-Control Studies; D015331:Cohort Studies; D046508:Culture Techniques; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology* / Q000601:surgery; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009367:Neoplasm Staging; D011336:Probability; D011379:Prognosis; D012016:Reference Values; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9