[Fusion expression of porcine IFN-gamma and Cysticercus cellulosae antigen cC1 in Escherichia coli cells].
OBJECTIVE
To construct an expression vector, including a chimeric cDNA of porcine IFN-gamma and Cysticercus cellulosae antigen cC1.


METHODS
DNA fragments of porcine IFN-gamma and cC1 including linkers were generated by polymerase chain reaction (PCR). The recombinant vector pJLA-PRcC1 was constructed by inserting a chimeric cDNA of porcine IFN-gamma and Cysticercus cellulosae antigen cC1, and transformed to E. coli XL-Blue.


RESULTS
The recombinant vector was identified by restriction analysis. An inserted fragment about 1.5 kb could be found. After induction, a 52 kDa new protein band appeared in SDS-PAGE.


CONCLUSIONS
The fusion expression of porcine IFN-gamma and cC1 antigen in Escherichia coli cells is successful.
Guo
Y
Y
Department of Medical Genetics, Second Military Medical University, Shanghai 200433.
Wu
D
D
Sun
S
S
D000818:Animals; D000947:Antigens, Helminth / Q000096:biosynthesis* / Q000235:genetics; D003552:Cysticercus / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005822:Genetic Vectors; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D013552:Swine
D000947:Antigens, Helminth; D011993:Recombinant Fusion Proteins; D007371:Interferon-gamma