[Determination of pravastatin in rat liver by RP-HPLC].
OBJECTIVE
To establish a reverse-phase high performance liquid chromatographic method for the determination of pravastatin in rat liver.


METHODS
An aliquot of 5 g liver homogenates, spiked with triamcinolone acetonide (internal standard), was extracted by solid-phase extraction with Bond Elut C18 columns. Chromatography was performed using a C18 reverse-phase column with mobile phase of Na2HPO4 buffer sdution (0.035 mmol.L-1, pH 3.0)-acetonitrile (155:42).


RESULTS
The linear equation was Y = 0.1843X-4.238 x 10(-3) (gamma = 0.9934) in the range of 0.05-10 micrograms.g-1 liver. The limit of detection for pravastatin was 13 ng.mL-1 (signal-to-noise ratio of 3), and the limit of quantification for pravastatin in liver homogenate was 50 ng.g-1 liver (RSD < 20%). The average extraction recovery of pravastatin from liver at different concentrations was 80.8%, and the average inter-day precision was 11%. This procedure was applied to assay pravastatin in rat liver which were collected from Lewis rats at different times after administration of pravastatin (ig 20 mg.kg-1).


CONCLUSIONS
The method was sensitive and is feasible for the pharmacokinetic and distribution study of pravastatin.
Li
X
X
Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310006, China.
Otter
K
K
Ziegler
A
A
D000818:Animals; D000924:Anticholesteremic Agents / Q000032:analysis / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D005260:Female; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000032:analysis / Q000493:pharmacokinetics; D008099:Liver / Q000737:chemistry*; D008297:Male; D017035:Pravastatin / Q000032:analysis* / Q000493:pharmacokinetics; D051381:Rats; D011917:Rats, Inbred Lew
D000924:Anticholesteremic Agents; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D017035:Pravastatin