[Protection of vascular endothelial cells from high glucose injury induced by quercetin].
OBJECTIVE
To evaluate the effects of quercetin on the high glucose-injured vascular endothelial cells (VECs).


METHODS
Experiments were divided into control group, injured group and quercetin group. The cultured VECs were injured by high glucose. The proliferation of VECs was assessed by MTT assay. The amount of NO and lipid peroxidation (monitored as maloraldehyde, MDA) of VECs were assessed by fluorometric assay. The amount of lactate dehydrogenase (LDH) was assessed by spectrophotometric method.


RESULTS
10(-2), 10(-3), 10(-4), 10(-5) mg/ml quercetin increased the proliferation of high glucose-injured VECs. 10(-2), 10(-3) mg/ml quercetin reduced LDH release and MDA production, increased NO release.


CONCLUSIONS
Quercetin can protect cultured VECs from being injured by high glucose.
Li
Guo
G
College of Life Science, East China Normal University, ShangHai 200062.
Zhang
Hongfeng
H
Li
Yiliang
Y
Wang
Yaofa
Y
D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005947:Glucose; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D009569:Nitric Oxide; D008315:Malondialdehyde; D011794:Quercetin; D007770:L-Lactate Dehydrogenase; D005947:Glucose