title: The schizontocidal activity of daphnetin against malaria parasites in vitro and in vivo.
abstract: OBJECTIVE
  To investigate the in vitro and in vivo schizontocidal activity of daphnetin.
  
  
  METHODS
  Schizontocidal activity of daphnetin was tested using an in vitro assay based on the routine in vitro cultivation of P. falciparum FCC1 strain. The in vivo antimalarial effects of daphnetin at various dosages were assessed in mice infected with P. b. erghei ANKA according to "4-day suppress assay".
  
  
  RESULTS
  In vitro, daphnetin exhibited potent schizontocidal activity comparable to chloroquine(CQ) at the dose range of 1-10 mumol/L. In vivo, 50 or 100 mg/kg.d-1 x 4 d daphnetin i.g. and 10, 50 or 100 mg/kg.d-1 x 4 d dephnetin i.p. showed antimalarial efficacy comparable to CQ 10 mg/kg.d-1 x 4 d i.g. in mice infected with P. berghei ANKA, evaluated by both the reduction rate of parasitemia on D4 and the average surviving days in 30 days.
  
  
  CONCLUSIONS
  Daphnetin displays certain schizontocidal activity both in vitro and in vivo.
authors.affiliation: Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
authors.forename: Q M
authors.initials: QM
authors.lastname: Wang
authors.forename: Y C
authors.initials: YC
authors.lastname: Ni
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Xu
authors.forename: S H
authors.initials: SH
authors.lastname: Ha
authors.forename: Y
authors.initials: Y
authors.lastname: Cai
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008288:Malaria / Q000188:drug therapy / Q000469:parasitology; D008297:Male; D051379:Mice; D010962:Plasmodium berghei / Q000187:drug effects*; D010963:Plasmodium falciparum / Q000187:drug effects*; D014468:Umbelliferones / Q000494:pharmacology*
chemical_list: D000962:Antimalarials; D014468:Umbelliferones; C039952:daphnetin