Effect of incubation temperature on the isolation of thermophilic species of Campylobacter from faeces.
Although no large-scale clinical study has been performed, it has been reported that incubation at 37 degrees C gives better isolation rates for all common species of Campylobacter than incubation at 42 degrees C, while also improving the recovery of the more unusual species. In this study, 2,570 faecal samples were examined in four laboratories, using a standardised protocol. Isolation rates of Campylobacter spp. were compared after incubation on modified campylobacter blood-free selective agar at 37 degrees C and 42 degrees C. Campylobacter spp. isolates were made from 185 samples (7.2%); 25 were recovered only at 42 degrees C and three only at 37 degrees C (p < 0.001). There were significantly more colonies at 42 degrees C (p = 0.001). Competing flora were heavier at 37 degrees C, but this did not account for the difference in isolation rates or quantity of growth. It is recommended that cultures for Campylobacter spp. be routinely incubated at 42 degrees C. More specific techniques are required to seek for fastidious campylobacters.
Gee
B
B
Public Health Laboratory Service (Midlands) Group Headquarters, Royal Shrewsbury Hospital. bgee@covphl.globalnet.co.uk
Nye
K J
KJ
Fallon
D
D
Messer
S
S
Howe
S
S
Warren
R E
RE
Andrews
N
N
D000362:Agar; D001431:Bacteriological Techniques / Q000379:methods; D002167:Campylobacter / Q000145:classification / Q000302:isolation & purification*; D016009:Chi-Square Distribution; D015169:Colony Count, Microbial; D005243:Feces / Q000382:microbiology*; D006801:Humans; D013696:Temperature
D000362:Agar