title: Identification of a vasopressin-like immunoreactive substance in hydra.
abstract: Vasopressin (VP)-like immunoreactivity has long been known in the hydra nervous system, but has not yet been structurally identified. In this study, using HPLC fractionation and an immunological assay, we have purified two peptides, FPQSFLPRGamide and SFLPRGamide, from Hydra magnipapillata. Both the peptides shared the same C-terminal structure, -PRGamide, with Arg-VP. The nonapeptide proved to be Hym-355, a peptide that stimulates neuronal differentiation in hydra. Detailed evaluation by competitive enzyme-linked immunosorbent assay (ELISA) and double immunostaining using anti-VP and anti-Hym-355 antibodies enabled us to conclude that the two peptides account for a major part of the VP-like immunoreactivity in hydra nerve cells.
authors.affiliation: Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739-8526, Japan. fumi425@hiroshima-u.ac.jp
authors.forename: F
authors.initials: F
authors.lastname: Morishita
authors.forename: Y
authors.initials: Y
authors.lastname: Nitagai
authors.forename: Y
authors.initials: Y
authors.lastname: Furukawa
authors.forename: O
authors.initials: O
authors.lastname: Matsushima
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Hatta
authors.forename: T
authors.initials: T
authors.lastname: Fujisawa
authors.forename: S
authors.initials: S
authors.lastname: Tunamoto
authors.forename: O
authors.initials: O
authors.lastname: Koizumi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D006829:Hydra / Q000737:chemistry*; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D014667:Vasopressins / Q000737:chemistry*
chemical_list: D014667:Vasopressins