Involvement of a quinoprotein (PQQ-containing) alcohol dehydrogenase in the degradation of polypropylene glycols by the bacterium Stenotrophomonas maltophilia.
Previous work has shown that when the bacterium Stenotrophomonas maltophilia is grown on polypropylene glycol, different dye-linked polypropylene glycol dehydrogenase (PPG-DH) activities are induced during growth. Here the purification and characterization of the dehydrogenase activity induced in the stationary phase, and present in the periplasmic space, is described. The homogeneous enzyme preparation obtained consists of a homodimeric protein with a molecular mass of about 123 kDa and an isoelectric point of 5.9. The cofactor of the enzyme appeared to be pyrroloquinoline quinone (PQQ), no heme c was present, and holo-enzyme contained two PQQ molecules per enzyme molecule. In these respects, PPG-DH described here is similar to already known quinoprotein alcohol dehydrogenases, but in other respects, it is different. Therefore, it is suggested that PPG-DH could be a new type of quinoprotein alcohol dehydrogenase. Based on its strong preference for polyols, PPG-DH seems well fitted to carry out the first step in the degradation of PPGs, synthetic polymers containing a variety of hydroxyl groups.
Tachibana
Shinjiro
S
Department of Bioscience, Faculty of Agriculture, University of the Ryukyus, 1 Senbaru, Nishihara-cho, 903-0213, Okinawa, Japan.
Kuba
Naoko
N
Kawai
Fusako
F
Duine
Johannis A
JA
Yasuda
Masaaki
M
D000426:Alcohol Dehydrogenase / Q000737:chemistry* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D045542:PQQ Cofactor; D011108:Polymers / Q000302:isolation & purification / Q000378:metabolism*; D011409:Propylene Glycols / Q000302:isolation & purification / Q000378:metabolism*; D015363:Quinolones / Q000032:analysis*; D011809:Quinones / Q000032:analysis* / Q000737:chemistry; D020615:Stenotrophomonas maltophilia / Q000201:enzymology* / Q000378:metabolism; D013379:Substrate Specificity
D011108:Polymers; D011409:Propylene Glycols; D015363:Quinolones; D011809:Quinones; C012504:polypropylene glycol; D045542:PQQ Cofactor; D000426:Alcohol Dehydrogenase