[Cloning and sequencing of the gp23 gene encoding a surface antigen on sporozoites of Cryptosporidium parvum].
OBJECTIVE
To clone and sequence the gp23 gene encoding a surface antigen on the sporozoites of Cryptosporidium parvum.


METHODS
Genomic DNA was isolated from oocysts of Cryptosporidium parvum. The gp23 gene was amplified by polymerase chain reaction (PCR) and cloned into pMD18-T vector and sequenced by the methods of dideoxy-mediated chain termination.


CONCLUSIONS
The gp23 gene was 345 bp in length and included an open reading frame encoding a protein of 114 amino acid residues. The homology of the nucleotide and amino sequences of the gp23 gene was 97.3% and 98.2% to that in the GenBank, respectively. The gp23 gene cloned contained 6 nucleotides more than that in the GenBank.
He
Hong-xuan
HX
Faculty of Animal Science, Quartermaster University of PLA, Changchun 130062.
Zhang
Xi-chen
XC
Ouyang
Hong-sheng
HS
Xu
Wei-dong
WD
Chen
Jian-bao
JB
Yin
Ji-gang
JG
Li
Jian-hua
JH
Yang
Ju
J
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics*; D000954:Antigens, Surface / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D016785:Cryptosporidium parvum / Q000235:genetics* / Q000276:immunology; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D016054:DNA, Protozoan