Dynamic relocalization of the chromosomal passenger complex proteins inner centromere protein (INCENP) and aurora-B kinase during male mouse meiosis.
INCENP and aurora-B kinase are two chromosomal passenger proteins that are thought to play key roles in coordinating chromosome segregation with cytokinesis in somatic cells. Here we have analyzed their subcellular distribution, and that of phosphorylated histone H3, and the timing of their relative appearance in mouse spermatocytes during both meiotic divisions. Our results show that in mitotic spermatogonial cells, INCENP and aurora-B show the same pattern of distribution as they do in cultured somatic cells. INCENP labels the synaptonemal complex central element from zygotene up to late pachytene when it begins to relocalize to heterochromatic chromocentres. Aurora-B first appears at chromocentres in late diplotene before the initial phosphorylation of histone H3. INCENP and aurora-B concentrate at centromeres during diakinesis and appear during metaphase I as T-shaped signals at their inner domains, just below associated sister kinetochores. During late anaphase I both proteins relocalize to the spindle midzone. Both proteins colocalize at a connecting strand traversing the centromere region and joining sister kinetochores, in metaphase II centromeres. This strand disappears at the metaphase II/anaphase II transition and relocalizes to the spindle midzone. We discuss the complex dynamic relocalization of the chromosomal passenger complex during prophase I. Additionally, we suggest that this complex may regulate sister-chromatid centromere cohesion during both meiotic divisions.
Parra
María Teresa
MT
Departamento de Biología, Edificio de Biológicas, Universidad Autónoma de Madrid, E-28049 Madrid, Spain.
Viera
Alberto
A
Gómez
Rocío
R
Page
Jesús
J
Carmena
Mar
M
Earnshaw
William C
WC
Rufas
Julio S
JS
Suja
José A
JA
D016475:3T3 Cells; D000705:Anaphase / Q000502:physiology; D000818:Animals; D064107:Aurora Kinase B; D064127:Aurora Kinases; D002503:Centromere / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D006367:HeLa Cells; D006570:Heterochromatin / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D018386:Kinetochores / Q000378:metabolism; D008297:Male; D008540:Meiosis / Q000502:physiology*; D008677:Metaphase / Q000502:physiology; D051379:Mice; D010766:Phosphorylation; D011418:Prophase / Q000502:physiology; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012854:Sister Chromatid Exchange / Q000502:physiology; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism*; D013573:Synaptonemal Complex / Q000378:metabolism
D002868:Chromosomal Proteins, Non-Histone; D006570:Heterochromatin; D006657:Histones; C083767:INCENP protein, human; C513185:Incenp protein, mouse; C576921:AURKB protein, human; C576924:Aurkb protein, mouse; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases