[Establishment of transgenic mouse models carrying human polymeric immunoglobulin receptor gene].
OBJECTIVE
To establish transgenic mouse models carrying human polymeric immunoglobulin receptor (pIgR) gene to render the mice prone to EBV infection in normal condition, thereby to facilitate the study of the role of Epstein-Barr virus (EBV) in the pathogenesis of nasopharyngeal carcinoma.


METHODS
By means of microinjection, pIgR gene under the regulation by kerotinocyte-specific promoter ED-L2 was introduced into the pronuclei of mouse zygote, which was transferred into pseudo- pregnant female mice to induce nasopharynx-specific pIgR expression in the founder mice.


RESULTS
Six out of the 16 founder mice (37.5%) was tested by PCR to be pIgR-positive, while Southern blotting identified only 2 positive mice.


CONCLUSIONS
Transgenic mice harboring pIgR gene can be used potentially as a new model for studying the pothogenesis of nasopharyngeal carcinoma by EBV.
Lü
Li-chun
LC
Institute of Cancer Research, First Military Medical University, Guangzhou 510515, China. lichunlu@fimmu.com
He
Ying
Y
Lan
Ke
K
Shen
Xin-ming
XM
Huang
Hua
H
Jiang
Pei-zhou
PZ
Wang
Shuang
S
Xu
Shou-jun
SJ
Yao
Kai-tai
KT
D000818:Animals; D020031:Epstein-Barr Virus Infections; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D019056:Receptors, Polymeric Immunoglobulin / Q000235:genetics* / Q000378:metabolism
D019056:Receptors, Polymeric Immunoglobulin