title: Antimetastatic and immunomodulating properties of a new herbal prescription, Bojung-bangam-tang.
abstract: We investigated the antimetastatic effect of Bojung-bangam-tang, a new herbal prescription, on liver metastasis by the inoculation of colon 26-L5 carcinoma cells into the portal vein. Oral administration of Bojung-bangam-tang for 15 days from day 7 before tumor inoculation significantly inhibited liver metastasis in a dose-dependent manner. Bojung-bangam-tang enhanced the mitogenic activity of BALB/c whole splenocytes induced by various mitogenic stimuli. Oral administration of Bojung-bangam-tang, by itself, could not induce the production of interleukin (IL)-12 or IFN-gamma by macrophages, but enhanced the potential of macrophages to produce cytokines in response to lipopolysaccharide (LPS). Experiments using macrophage- or natural killer (NK) cell-deficient mice revealed that the antimetastatic effect of Bojung-bangam-tang is mediated by macrophages rather than NK cells. Bojung-bangam-tang caused a marked increase of production of Th1 cytokine (IFN-gamma) and decrease of production of Th2 cytokine (IL-4) by splenocytes stimulated with concanavalin A (Con A). These results indicated that oral administration of Bojung-bangam-tang inhibited the liver metastasis of colon 26-L5 cells through a mechanism leading to a Th1 dominant immune state and activation of macrophages. Thus, Bojung-bangam-tang may be useful for the prevention of cancer metastasis.
authors.affiliation: Department of Pathogenic Biochemistry, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 2630, Sugitani, Toyama 930-0194, Japan.
authors.forename: Soo Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Ikuo
authors.initials: I
authors.lastname: Saiki
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hayakawa
authors.forename: Shinyu
authors.initials: S
authors.lastname: Nunome
authors.forename: Haruki
authors.initials: H
authors.lastname: Yamada
authors.forename: Sung-Hoon
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D008114:Liver Neoplasms, Experimental / Q000517:prevention & control / Q000556:secondary; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D014407:Tumor Cells, Cultured
chemical_list: D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D018664:Interleukin-12; D007371:Interferon-gamma