[Apoptosis inductive effect of homoharringtonine and isoharringtonine on human acute promyelocytic leukemia HL-60 cells].
OBJECTIVE
To study the effects of homoharringtonine and isoharringtonine on the induction of apoptosis on human promyelocytic leukemia HL-60 cells.


METHODS
By using flow cytometry, DNA gel electrophoresis, microscopy and electron microscopy.


RESULTS
It was demonstrated that homoharringtonine and isoharringtonine can induce apoptosis of HL-60 cells significantly and rapidly. The activity of homoharringtonine was higher than that of isoharringtonine, and it was time and concentration dependent.


CONCLUSIONS
Both homoharringtonine and isoharringtonine could induce apoptosis of HL-60 cells in terms of morphology, and DNA ladder assay. The activity of homoharringtonine was much higher than that of isoharringtonine.
Shi
B
B
Department of Pharmacology, Institute of Materia Medica, CAMS and PUMC, Beijing 100050.
Han
R
R
D017209:Apoptosis / Q000187:drug effects*; D018922:HL-60 Cells / Q000187:drug effects*; D006248:Harringtonines / Q000494:pharmacology*; D000077863:Homoharringtonine; D006801:Humans
D006248:Harringtonines; C001656:isoharringtonine; D000077863:Homoharringtonine