title: Optimization and validation of a micellar electrokinetic chromatographic method for the analysis of several angiotensin-II-receptor antagonists.
abstract: We have optimized a micellar electrokinetic capillary chromatographic method for the separation of six angiotensin-II-receptor antagonists (ARA-IIs): candesartan, eprosartan mesylate, irbesartan, losartan potassium, telmisartan, and valsartan. A face-centred central composite design was applied to study the effect of the pH, the molarity of the running buffer, and the concentration of the micelle-forming agent on the separation properties. A combination of the studied parameters permitted the separation of the six ARA-IIs, which was best carried out using a 55-mM sodium phosphate buffer solution (pH 6.5) containing 15 mM of sodium dodecyl sulfate. The same system can also be applied for the quantitative determination of these compounds, but only for the more stable ARA-IIs (candesartan, eprosartan mesylate, losartan potassium, and valsartan). Some system parameters (linearity, precision, and accuracy) were validated.
authors.affiliation: Laboratory of Pharmaceutical Chemistry and Drug Analysis, Department of Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium. sandra.hillaert@rug.ac.be
authors.forename: S
authors.initials: S
authors.lastname: Hillaert
authors.forename: T R M
authors.initials: TR
authors.lastname: De Beer
authors.forename: J O
authors.initials: JO
authors.lastname: De Beer
authors.forename: W
authors.initials: W
authors.lastname: Van den Bossche
mesh_terms: D000804:Angiotensin II / Q000378:metabolism*; D057911:Angiotensin Receptor Antagonists; D000959:Antihypertensive Agents / Q000032:analysis* / Q000494:pharmacology; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011945:Receptors, Angiotensin / Q000378:metabolism; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D057911:Angiotensin Receptor Antagonists; D000959:Antihypertensive Agents; D002021:Buffers; D011945:Receptors, Angiotensin; D000804:Angiotensin II