title: [Effect of dingxin recipe on intracellular free calcium, membrane potential and mitochondria membrane potential in normal and hypoxia myocytes of rats].
abstract: OBJECTIVE
  To elucidate the molecular mechanism on anti-arrhythmic function of Dingxin Recipe (DXR).
  
  
  METHODS
  Cardial myocytes isolated by trypsin method were cultured and labelled with various fluorescence stains. DXR contained serum induced changes of cytolic calcium concentration [Ca2+]i, cell membrane potential (MP) and mitochondria membrane potential (MMP) were determined with laser confocal microscopy.
  
  
  RESULTS
  Hypoxia caused increase of [Ca2+]i and MMP, and decrease of MP, while DXR contained serum lowered the [Ca2+]i in normal or hypoxian myocytes, improved hypoxia induced MP reduction and maintain MMP at baseline level under hypoxia circumstances.
  
  
  CONCLUSIONS
  Through inhibiting the hypoxia caused [Ca2+]i and MMP elevation and MP lowering, DXR displays its action in protecting myocardial cells to prevent and treat arrhythmia.
authors.affiliation: Faculty of TCM, First Military Medical University, Guangzhou 510515.
authors.forename: Y H
authors.initials: YH
authors.lastname: Jia
authors.forename: X G
authors.initials: XG
authors.lastname: Sun
authors.forename: X S
authors.initials: XS
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D001145:Arrhythmias, Cardiac / Q000517:prevention & control; D002118:Calcium / Q000378:metabolism*; D015687:Cell Hypoxia / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000502:physiology; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000889:Anti-Arrhythmia Agents; D004365:Drugs, Chinese Herbal; D002118:Calcium