[HPLC quantitative analysis of berberine absorpted with macroporous resin in Rhizoma Coptidis and its preparation zuo jin wan].
Absorptive ability and elution program of macroporous resin to the berberine in the Rhizoma Coptidis and its preparation Zuo Jin Wan (containing Rhizoma Coptidis and Fructus Evodiae) were explored. The extract of 70% MeOH of samples were disolved in water, then alkalized to pH 12 with ammonia solution. The berberine was absorpted by the macroporous resin, the washed off water soluble impurity with alkalescent water, and eluted with 50% MeOH containing 0.5% H2SO4. The results of quantitative analysis of the berberine by HPLC were satisfied.
Luo
J
J
Guangdong College of Pharmacy, Guangzhou 510224.
Ma
H
H
Xu
M
M
Cheng
X
X
D000327:Adsorption; D001599:Berberine / Q000032:analysis*; D001600:Berberine Alkaloids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D000089042:Coptis chinensis; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D011108:Polymers / Q000737:chemistry*; D012117:Resins, Synthetic
D001600:Berberine Alkaloids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D011108:Polymers; D012117:Resins, Synthetic; D001599:Berberine; C107393:Coptidis rhizoma extract