[Construction of DNA vaccine including a chimeric gene encoding flagellin and outer membrane protein antigen from Leptospira interrogons serovar lai].
OBJECTIVE
To construct a fusion expression vector for DNA vaccine, including a flagellin gene (flaB2) and an outer membrane protein gene (ompL1) to enhance the protective immunity of Leptospira interrogans serova Lai (Stain 017).


METHODS
The DNA fragments encoding flaB2 and ompL1 were amplified respectively by PCR and then fused. The obtained chimeric gene ompL1-flaB2 contained a synthetic linker of ten amino acids to sustain its space conformation.


RESULTS
Identified by restriction enzyme analysis, an insert fragment of 1.8 kb was demonstrated. FlaB2 and ompL1 respectively had the same sequence as reported by DNA sequencing analysis.


CONCLUSIONS
A fusion expression plasmid containing flaB2 and ompL1 was constructed.
Wang
Min
M
Research Unit of Leptospirosis, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Dai
Baomin
B
You
Zill
Z
Fang
Zhimao
Z
Wang
Yajing
Y
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D005408:Flagellin / Q000235:genetics / Q000276:immunology*; D007921:Leptospira interrogans / Q000235:genetics* / Q000276:immunology; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D019444:Vaccines, DNA / Q000096:biosynthesis* / Q000276:immunology
D001425:Bacterial Outer Membrane Proteins; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D005408:Flagellin