title: [Purification and immunogenicity identification of the recombinant protein related with specific IgE against Schistosoma japonicum].
abstract: OBJECTIVE
  To purify the specific IgE antibody-related recombinant protein of Schistosoma japonicum and to identify its immunogenicity.
  
  
  METHODS
  The recombinant plasmid Sj43B/pGEX-6p-1 was expressed in E. coli BL 21. The inclusion body of the fusion protein was washed by TNMFX buffer and separated by FPLC. After renaturation, the fusion protein was used to vaccinate the mice. The specific IgG and IgE antibodies were detected by dot-ELISA and Western blotting analysis, respectively.
  
  
  RESULTS
  Most of the proteins mixed with the inclusion body of the recombinant protein could be eliminated by washing with TNMFX buffer. The purified recombinant fusion protein could be obtained by FPLC separation. The experiment on mice immunized with the fusion protein showed that the specific IgE antibody was generated against the target part of the fusion protein, but not the specific IgG antibody.
  
  
  CONCLUSIONS
  The fusion protein expressed by the recombinant plasmid Sj43B/pGEX-6p-1 could induce specific IgE response of the immunized mice.
authors.affiliation: Laboratory of Molecular Immunoparasitology, Institute of Medical Molecular Biology, Nanjing Medical University, Nanjing 210029.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Chuan
authors.initials: C
authors.lastname: Su
authors.forename: Zhao-song
authors.initials: ZS
authors.lastname: Zhang
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Hu
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Chun-lin
authors.initials: CL
authors.lastname: Li
authors.forename: Min-jun
authors.initials: MJ
authors.lastname: Ji
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhu
authors.forename: Guan-ling
authors.initials: GL
authors.lastname: Wu
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D015801:Helminth Proteins / Q000276:immunology* / Q000302:isolation & purification; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000276:immunology* / Q000302:isolation & purification; D012549:Schistosoma japonicum / Q000276:immunology*; D014611:Vaccination
chemical_list: D000909:Antibodies, Helminth; D015801:Helminth Proteins; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D007073:Immunoglobulin E