[Complete Freund's adjuvant-induced splenic T cell apoptosis in the prevention of diabetes in non-obese diabetic mice].
OBJECTIVE
To study the apoptosis of T lymphocytes in the spleen and its role in complete Freund's adjuvant (CFA) preventing diabetes in non-obese diabetic (NOD) mice.


METHODS
Forty-two NOD mice were divided into two groups randomly: CFA-treated NOD female mice (6w, 12w and 30w) and PBS-treated NOD female mice (6w, 12w and 30w). The apoptosis of splenic T lymphocytes was observed with the TDT-mediated fluorescein-dUTP nick-end labelling (TUNEL) technique and avidin-biotin complex method.


RESULTS
CFA induced the apoptosis of CD4+ T lymphocytes in the mouse spleen and could prevent the development of Type 1 diabetes (T1DM) in NOD female mice.


CONCLUSIONS
CFA preventing Type 1 diabetes in NOD mice may be related to the apoptosis of T lymphocytes in the spleen, which downregulates mature CD4+ T lymphocytes in the periphery.
Wang
Xiao-xi
XX
Department of Endocrinology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Niu
Xiao-hong
XH
Zhou
Zhi-guang
ZG
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology*; D051379:Mice; D016688:Mice, Inbred NOD; D011897:Random Allocation; D013154:Spleen / Q000166:cytology
D005620:Freund's Adjuvant