Identified circadian rhythm genes of ciliary epithelium with differential display.
OBJECTIVE
To identify differential genes expressed in the rabbit ciliary epithelium during the circadian cycle of aqueous flow.


METHODS
Total RNA from ciliary epithelium of rabbits at 8 AM (light on 1 hour) and 8 PM (light off 1 hour) were compared by differential display reverse transcription-polymerase chain reaction (DD RT-PCR), using 6% denaturing polyacrylamide electrophoresis, choose differential display bands, cut and reamplify with the same primer, clone and sequence. Search the database of Genbank, prolong them with 5' RACE and 3' RACE technique then clone, sequence and search database of Genbank.


RESULTS
93 Significant differences gene expression were detected between light on and light off in the rabbit ciliary epithelium.


CONCLUSIONS
Differential display is a powerful tool to screen differentially expressed genes in circadian rhythm of ciliary epithelium.
Li
Y
Y
Yale University School of Medicine, New Haven, CT 06520.
Lu
D
D
Ge
J
J
Li
Y
Y
Zhuo
Y
Y
Sears
M L
ML
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism / Q000502:physiology*; D002924:Ciliary Body / Q000166:cytology*; D002940:Circadian Rhythm / Q000235:genetics*; D004848:Epithelium / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods