title: Involvement of NF-Y and Sp1 binding sequences in basal transcription of the human telomerase RNA gene.
abstract: The proximal promoter of the telomerase RNA gene, hTR, contains four Sp1 sites and one CCAAT box. We have carried out a functional analysis of the role of these sequence elements. Two Sp1 sites downstream of the CCAAT box mediated negative regulation, while the other two Sp1 sites were positive regulators with the strongest effect mediated by the negative regulatory Sp1 site closely flanking the CCAAT box. Basal transcriptional activity is maintained via the CCAAT box even when all four Sp1 sites are mutated, suggesting nuclear factor-Y (NF-Y) is a fundamental regulator of hTR promoter function. Chromatin immunoprecipitation revealed binding of NF-Y, Sp1 and TFIIB to the promoter in vivo. Thus the interaction of NF-Y at the CCAAT box is pivotal to hTR gene transcription and surrounding sequence elements may provide an environment for the regulation of activity through recruitment of additional protein complexes.
authors.affiliation: Cancer Research UK Department of Medical Oncology, University of Glasgow, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
authors.forename: Jiangqin
authors.initials: J
authors.lastname: Zhao
authors.forename: Alan
authors.initials: A
authors.lastname: Bilsland
authors.forename: Stacey F
authors.initials: SF
authors.lastname: Hoare
authors.forename: W Nicol
authors.initials: WN
authors.lastname: Keith
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D023081:CCAAT-Binding Factor / Q000378:metabolism*; D006801:Humans; D009154:Mutation; D011401:Promoter Regions, Genetic; D012313:RNA; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000235:genetics*; D020218:Response Elements; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D019098:Telomerase / Q000235:genetics*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D023081:CCAAT-Binding Factor; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D016329:Sp1 Transcription Factor; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase