title: Protective effects of Wuyaoshunqisan against H2O2-induced apoptosis on hippocampal cell line HiB5.
abstract: Oxidative stress has been implicated in the pathogenesis of different neurodegenerative disorders. To investigate the protective effects of Wuyaoshunqisan against H2O2-induced apoptosis in the central nervous system, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) method, flow cytometric analysis, and the DNA fragmentation assay were performed on cells of the hippocampal cell line HiB5. Through the morphological and biochemical analyses, it was shown that HiB5 cells treated with H2O2 exhibit classical apoptotic features, while the occurrence of such changes is reduced in cells pre-treated with Wuyaoshunqisan prior to H2O2 exposure.
authors.affiliation: Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: Jeong-Jin
authors.initials: JJ
authors.lastname: Je
authors.forename: Hyun-Taeg
authors.initials: HT
authors.lastname: Shin
authors.forename: Seok-Hee
authors.initials: SH
authors.lastname: Chung
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Lee
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Kim
authors.forename: Hyun-Dae
authors.initials: HD
authors.lastname: Shin
authors.forename: Mi-Hyeon
authors.initials: MH
authors.lastname: Jang
authors.forename: Youn-Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Joo-Ho
authors.initials: JH
authors.lastname: Chung
authors.forename: Ee-Hwa
authors.initials: EH
authors.lastname: Kim
authors.forename: Chang-Ju
authors.initials: CJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D005434:Flow Cytometry; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D020287:In Situ Nick-End Labeling; D007202:Indicators and Reagents; D016877:Oxidants / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000975:Antioxidants; D007202:Indicators and Reagents; D016877:Oxidants; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; C473660:wuyaoshunqisan; D006861:Hydrogen Peroxide; C022616:thiazolyl blue