A simple method of DNA extraction and STR typing from urine samples using a commercially available DNA/RNA extraction kit.
We devised a simple DNA extraction procedure suitable for STR typing of urine sample. Use of a commercially available DNA/RNA extraction kit equipped with a silica-gel-based membrane made it possible to omit the recovery of urinary nucleated cells by sedimentation before the extraction. Successful genotyping of the TH01, HumTPO and multiplex STRs was achieved using aliquots of urine as small as 100 microL. Furthermore, application of this DNA extraction procedure to frozen urine samples provided STR allele results comparable to results obtained from fresh samples. Therefore, this extraction procedure is considered to be effective for STR typing of urine samples in both the frozen and aqueous state. Furthermore, addition of sodium azide to fresh urine samples prolonged their storage duration even at room temperature.
Yasuda
Toshihiro
T
Department of Biology, Fukui Medical University, Fukui, Japan.
Iida
Reiko
R
Takeshita
Haruo
H
Ueki
Misuzu
M
Nakajima
Tamiko
T
Kaneko
Yasushi
Y
Mogi
Kouichi
K
Tsukahara
Tetsuya
T
Kishi
Koichiro
K
D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods; D005838:Genotype; D006801:Humans; D011933:Reagent Kits, Diagnostic; D020080:Tandem Repeat Sequences; D016482:Urinalysis
D011933:Reagent Kits, Diagnostic; D004247:DNA