[Cloning of cDNA fragments of human defensins].
In order to acquire the cDNA of a small molecular peptide with biological activity, the cDNA encoding human defensins was isolated from total RNAs of peripheral leukocyte in a patient with chronic myelogenous leukemia by the RT-PCR method, about 280 bp in length. The RT-PCR products were cloned into the pUCm-T vector, and may be used to construct a novel fusion protein, which contains human defensins and human bactericidal permeability increasing proteins.
Liu
Shui-ping
SP
Molecular Biology Research Center, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Yang
Yu
Y
Yi
Wei-feng
WF
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D023082:Defensins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism*
D018076:DNA, Complementary; D023082:Defensins