[Effect of garlicin on adhesion molecules expression and deformability of peripheral neutrophils in patients with acute cerebral infarction].
OBJECTIVE
To observe the effect of garlicin on adhension molecules CD11a and deformability of peripheral neutrophil in patients with acute cerebral infarction (ACI).


METHODS
Neutrophils were separated from peripheral blood of healthy subjects and ACI patients, and incubated in 37 degrees C in vitro. The CD11a expression was detected by antibody fluorescence labeling method and the time of neutrophils passing millipore membrane were measured for calculation of the filter index.


RESULTS
CD11a expression rate in healthy subjects was 34.64 +/- 25.34%, while in patients was 55.35 +/- 30.54%, difference between them was significant (P < 0.05). After garlicin treatment, it lowered to 49.16 +/- 31.68%, as compared with untreated group, P < 0.05. The neutrophil filter index in healthy group, untreated group, garlicin treated group and Nimodipine treated group was 0.87 +/- 0.46, 6.42 +/- 6.40, 3.47 +/- 3.67 and 5.03 +/- 3.72 respectively, comparison between that in the garlicin treated group and in untreated group showed significant difference (P < 0.05).


CONCLUSIONS
Garlicin could effectively inhibit the CD11a expression in peripheral blood neutrophils and improve the deformability of the neutrophils in ACI patients.
Zhang
Jiu-liang
JL
China-Japan Friendship Hospital, Beijing 100029.
Sun
Rui-juan
RJ
Shi
Zai-xiang
ZX
D000368:Aged; D000498:Allyl Compounds / Q000494:pharmacology*; D040881:CD11a Antigen / Q000096:biosynthesis*; D002448:Cell Adhesion / Q000187:drug effects*; D002469:Cell Separation; D002544:Cerebral Infarction / Q000097:blood*; D004220:Disulfides / Q000494:pharmacology*; D004907:Erythrocyte Deformability / Q000187:drug effects*; D005260:Female; D005737:Garlic / Q000737:chemistry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology*
D000498:Allyl Compounds; D040881:CD11a Antigen; D004220:Disulfides; C028009:diallyl disulfide