[Structure characteristics of insulin liposomes].
OBJECTIVE
To study the molecular location and state of insulin within insulin-lipids.


METHODS
Particle size and distribution of insulin-lipids was determined by using laser light scattering instrument, and transmission electron microscopy (TEM) was used to examine the morphology of insulin-lipids. HPLC, fluorescence scanning, digestion with trypsin and polyacrylamide gel electrophoresis was used to determine the molecular localization and state of insulin in the lipids.


RESULTS
The mean particle size, polydispersity and sample quality were 218.3 nm, 0.073 and 7.2 respectively. The results of HPLC determination showed insulin exist in the insulin-lipids. There was no insulin emission spectrum in the insulin-lipids fluorescence spectrum; nearly no insulin digested in the test of insulin digestion by trypsin, and no insulin band in the insulin-lipids in figure of polyacrylamide gel electrophoresis.


CONCLUSIONS
Insulin was encapsulated within lipids.
Zhang
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Department of Pharmaceutics, School of Pharmaceutical Science, Peking University, Beijing 100083, China.
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D002851:Chromatography, High Pressure Liquid; D007328:Insulin / Q000008:administration & dosage / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008854:Microscopy, Electron; D010316:Particle Size; D013678:Technology, Pharmaceutical; D014357:Trypsin / Q000378:metabolism
D007328:Insulin; D008081:Liposomes; D014357:Trypsin