Comparison of separation conditions and ionization methods for the liquid chromatography-mass spectrometric determination of sulfonamides.
The effects of the type and concentration of buffer, composition of the mobile phase and the ionization mode, used for the separation and detection of sulfonamides with LC-MS, were studied. Five typical sulfonamides were selected as target compounds and beef meat was selected as a matrix sample. For the separation of sulfonamides, 0.05 M NH4Ac in 13-15% aqueous acetonitrile, APCl ionization was more effective than ESI with regard to separation efficiency and the detection sensitivity.
Kim
Dal-Ho
DH
Organic Analysis Laboratory, Division of Chemical Metrology and Materials Evaluation, Korea Research Institute of standards and Science, Yusong, Daejon 305-600, Republic of Korea. dhkim@kriss.re.kr
Lee
Dai Woon
DW
D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013449:Sulfonamides / Q000032:analysis*
D013449:Sulfonamides