Application of cDNA microarray technique to detection of gene expression in host cells infected with viruses.
cDNA microarray technique was used to monitor changes in mRNA levels in cells after Hantaan virus (HTNV) infection. The values of the ratio of medians for HTNV and Japanese encephalitis virus (JEV) at the early stage of infection were compared and found similar, suggesting that the same or similar genes are associated with the early events of infection with either virus. The reproducibility of values of the "ratio of medians" for HTNV was examined. We found that applying cluster analysis to the gene expression data groups efficiently together genes with the same function. Therefore, in analyzing the effects of viral infection on host cells by the cDNA microarray technique, clustering data appear to be necessary for gaining biological meaning from a dump of gene expression profiles obtained from virus-infected cells.
Nam
J H
JH
Department of Virology, Korean National Institute of Health, 5 Nokbun-dong, Eunpyung-gu, Seoul, 122-701, Korea. Jnam66@yahoo.com
Yu
C H
CH
Hwang
K A
KA
Kim
S
S
Ahn
S H
SH
Shin
J Y
JY
Choi
W Y
WY
Joo
Y R
YR
Park
K Y
KY
D000818:Animals; D002522:Chlorocebus aethiops; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D018063:Hantaan virus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010802:Phylogeny; D012367:RNA, Viral / Q000096:biosynthesis*; D015203:Reproducibility of Results; D014709:Vero Cells
D012367:RNA, Viral