title: Converting the sacrificial DNA repair protein N-ada into a catalytic methyl phosphotriester repair enzyme.
abstract: Mutation of the active-site residue Cys38 of N-Ada converts it from a sacrificial DNA repair protein to an enzyme that uses methanethiol as an external sacrificial reagent to repair DNA methyl phosphotriesters catalytically.
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St., Cambridge, Massachusetts 02138, USA.
authors.forename: Chuan
authors.initials: C
authors.lastname: He
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Verdine
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D002384:Catalysis; D011088:DNA Ligases / Q000737:chemistry* / Q000378:metabolism*; D004260:DNA Repair; D029968:Escherichia coli Proteins; D019853:O(6)-Methylguanine-DNA Methyltransferase; D010755:Organophosphates / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D010755:Organophosphates; D014157:Transcription Factors; C045675:Ada protein, E coli; D019853:O(6)-Methylguanine-DNA Methyltransferase; D011088:DNA Ligases