Analysis of pyridines in mainstream cigarette smoke.
A new technique has been developed for the quantitative analysis of pyridines in mainstream cigarette smoke using a GC-MS technique. For analysis, 10 cigarettes are smoked using conditions based on US Federal Trade Commission recommendations. The smoke is collected in a water trap and analyzed using a GC-MS technique. A standard or a fast GC separation can be applied for the analysis. The standard separation was followed by MS detection using selected ion monitoring (SIM) acquisition on a quadrupole instrument. The fast GC was followed by MS detection with total ion acquisition on a time-of-flight instrument. The levels of pyridine depend on the type of cigarette: for a full flavor cigarette pyridine is as high as 18.0 microg/cigarette (cig.). and for an ultra light cigarette is about 3.0 microg/cig. Substituted pyridines vary between 5.0 microg/cig. to 0.1 microg/cig. for a full flavor cigarette, and between 0.2 microg/cig. and a few ng/cig. for an ultra light cigarette. The reproducibility of the technique is very good, with less than 7-8% RSD in both separation procedures for most of the analyzed compounds.
Kulshreshtha
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Brown & Williamson Tobacco Corp., 2600 Weaver Road, Macon, GA 31217, USA.
Moldoveanu
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SC
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D011725:Pyridines / Q000032:analysis*; D015203:Reproducibility of Results; D012906:Smoke / Q000032:analysis*; D014026:Nicotiana / Q000737:chemistry*
D011725:Pyridines; D012906:Smoke