[The Role of Calcium Ion in Apotopsis of HL-60 Cells Induced by VP-16]
To study the significance of calcium in the apoptosis of HL-60 cells induced by VP-16, the technology of flow cytometry, confocal laser scanning microscopy and Western blot were used. The results showed that VP-16 could induced the apoptosis of HL-60 cells and transient increase of intracellular calcium concentration; EGTA [ethylene glycol-bis(2-aminoethyl)-N,N,N',N'-tetraacetic acid], that could combine the extracellular calcium, did not prevent the apoptosis of HL-60 cells. BAPTA-AM [1,2-bis(2-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid tetrakis (acetoxy-methyl) ester], however, a chelating agent of intracellular calcium ions, could prevent apoptosis and the release of cytochrome C from HL-60 cells. It was concluded that the calcium plays an important role in apoptosis and the release of cytochrome C.
Shi
Hongxia
H
Institute of Hematology, Beijing Medical University, Beijing 100044, China; Medicine and Pharmacology Analysis Centre, Peking University, Beijing 100084, China.
Li
Li
L
Yuan
Lan
L
He
Qihua
Q
Wang
Shenwu
S
Wang
Debing
D