title: [Development of Acute Promyelocytic Leukemia in PML-RARalpha Transgenic Mice]
abstract: To investigate the leukemogenic potential of PML-RARalpha fusion protein in vivo, hCG-PML-RARalpha transgene was constructed using molecular cloning technique and hCG-PML-RARalpha transgenic mice were generated. The genotype and phenotype of hCG-PML-RARalpha transgenic mice were analyzed by PCR, RT-PCR, morphology of peripheral blood and bone marrow cells, and pathological examination of spleen, liver and bone marrow. As a result, acute promyelocytic leukemia was developed in 3 hCG-PML-RARalpha transgenic mice in 1 - 5 months. The results demonstrated that PML-RARalpha fusion protein plays a crucial role in leukemogenesis.
authors.affiliation: Shanghai Institute of Hematology, Affiliated Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China; Shanghai Railway Center Hospital, Shanghai 200072, China.
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Meng
authors.forename: Guoxiang
authors.initials: G
authors.lastname: Cheng
authors.forename: Wenjun
authors.initials: W
authors.lastname: Cao
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
authors.forename: Jianquan
authors.initials: J
authors.lastname: Chen
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
authors.forename: Saijuan
authors.initials: S
authors.lastname: Chen