Human brain endothelial cells supply support for monocyte immunoregulatory functions.
Blood-derived monocytic cells comprise a significant component of most inflammatory responses that occur in the CNS. We utilized human brain-derived endothelial cells (HBECs) coated membranes in Boyden chambers to assess immune function related properties of human blood-derived monocytes following interaction with HBECs. Monocytes in contact with HBECs maintained functional antigen-presenting capacity and chemokine/cytokine production in contrast to monocytes that migrated through the HBEC barrier. These results indicate that HBECs, although themselves incapable of serving as competent antigen-presenting cells during the course of inflammatory CNS disorders, supply support needed for infiltrating perivascular monocytes to maintain their functions. Monocyte migration across HBECs was inhibited by interferon-beta.
Séguin
Rosanne
R
Neuroimmunology Unit, Montréal Neurological Institute, 3801 University, H3B 2A4, Montreal, Quebec, Canada.
Moditi
Zacharo
Z
Rotondo
Ronny
R
Biernacki
Katarzyna
K
Wosik
Karolina
K
Prat
Alexandre
A
Antel
Jack P
JP
D000328:Adult; D017951:Antigen Presentation; D001253:Astrocytes / Q000502:physiology; D001921:Brain / Q000098:blood supply*; D002450:Cell Communication; D002465:Cell Movement; D002470:Cell Survival; D002478:Cells, Cultured; D018925:Chemokines / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology; D016753:Interleukin-10 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000276:immunology*; D020932:Nerve Growth Factor / Q000494:pharmacology
D018925:Chemokines; D016207:Cytokines; D008070:Lipopolysaccharides; D016753:Interleukin-10; D016899:Interferon-beta; D020932:Nerve Growth Factor