[Sequence analysis of SSU rDNA variable regions of Leishmania isolates from hilly foci and plain foci of China].
OBJECTIVE
To analyze the sequence difference of the SSU rDNA variable regions of Leishmania isolates from hilly foci and plain foci of China.


METHODS
Specific SSU rDNA fragments from nuclear DNA of five Leishmania species and isolates were amplified by PCR. The amplified DNA fragments were cloned into pGEMR-T Easy vector. The specific fragments were sequenced by the automated DNA sequencer.


RESULTS
Sequence analysis showed that the amplified DNA fragments of five Leishmania species and isolates were all 392 bp in length, point mutations were located in the two unique sequence (UQ-I and UQ-II); L. d. SC10 and L. d. GS7 had two same point mutations in UQ-II, only L. d. GS7 had one in UQ-I; no insertion/deletion.


CONCLUSIONS
Sequence difference of the SSU rDNA variable region existed between Leishmania isolates from hilly foci and plain foci; The sequences of the SSU rDNA variable regions of L. d. SD2 isolate and L. infantum were identical.
Bu
L Y
LY
Department of Parasitology, West China University of Medical Sciences, Chengdu 610041.
Hu
X S
XS
Jing
B Q
BQ
Yi
T L
TL
D000818:Animals; D016054:DNA, Protozoan / Q000737:chemistry*; D004275:DNA, Ribosomal / Q000737:chemistry*; D006801:Humans; D007893:Leishmania donovani / Q000235:genetics*; D007898:Leishmaniasis, Visceral / Q000469:parasitology*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D004275:DNA, Ribosomal