[Comparison between apoptosis cells count and ATP-bioluminescence assay in ovarian cancer chemosensitivity testing in vitro].
OBJECTIVE
This study was aimed to evaluate the potentiality for the use of apoptosis cells count as a chemosensitivity testing in vitro for ovarian cancer.


METHODS
An end-labelling assay was used to determine the apoptosis cells of 4 specimens of fresh ovarian cancer cells and the results were compared with that of ATP assay.


RESULTS
It was found that chemotherapy can induce apoptosis, and the same chemotherapeutic agent can induce different apoptosis cells for different cancers.


CONCLUSIONS
The observed agreement between apoptosis cells count and ATP assay was 0.75. The above data demonstrate that apoptosis cells count can be used as a chemosensitivity testing for ovarian cancer, and a combination of these two methods may improve the clinical effects of chemotherapeutic agents.
Lou
Jiangyan
J
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Wang
He
H
Liu
Shanling
S
Peng
Zhilan
Z
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004317:Doxorubicin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D004317:Doxorubicin; D005472:Fluorouracil