Identification and isolation of foot-and-mouth disease virus from primary suspect cases in Korea in 2000.
The Republic of Korea had been free from foot and mouth disease (FMD) since 1934, until a recent outbreak in 2000. From March to April 2000, a total of 15 FMD outbreaks due to the serotype O virus were recorded. Coincidental outbreaks of FMD in cattle or pigs by the serotype O virus were reported in the region, including Taiwan, China, Japan, Russia and Mongolia. In this report, the results of emergency investigations of FMD cases on a dairy farm located approximately 5-km from the demilitarized zone in Korea are described. The causative agent of the disease was identified as the FMD virus O by reverse transcription-polymerase chain reaction (RT-PCR) assays using primers derived from the 3D polymerase, internal ribosome entry site (IRES), 1D/2B regions, enzyme-linked immunosorbent assay (ELISA) for antigen detection and typing. Sequence data of the partial 1D/2B region obtained from vesicular fluid showed close similarity (98% sequence identity) to the Kinmen isolate of the FMD virus O in Taiwan. The causative virus was isolated using black goat fetal lung cells following propagation in unweaned mice.
Shin
Jin-Ho
JH
National Veterinary Research and Quarantine Service, Anyang, Gyeonggi, Republic of Korea.
Sohn
Hyun-Joo
HJ
Choi
Kang-Seuk
KS
Kwon
Byung-Jun
BJ
Choi
Cheong-Up
CU
Kim
Jae-Hoon
JH
Hwang
Eui-Kyung
EK
Park
Jong-Hyeon
JH
Kim
Jong-Yeom
JY
Choi
Sang-Ho
SH
Kim
Ok-Kyung
OK
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000276:immunology / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D004196:Disease Outbreaks; D005260:Female; D005536:Foot-and-Mouth Disease / Q000453:epidemiology* / Q000276:immunology / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007723:Korea / Q000453:epidemiology; D012463:Saliva / Q000821:virology
D000956:Antigens, Viral; D004279:DNA, Viral