[Fractionation and identification of Schistosoma japonicum adult worm 67kD protein].
OBJECTIVE
To isolate the specific protein bands which react to sera from rabbits infected with Schistosoma japonicum (Sj) and those immunized with Sj adult worm antigens (AWA) for immunodiagnosis of schistosomiasis.


METHODS
AWA was analysed using SDS-PAGE and immunoblotting, and 67 kD protein was fractionated by electrophoretic chromatography.


RESULTS
67 kD protein which reacted to rabbit sera infected with Sj and immunized with AWA was obtained after the fractionation of AWA through electrophoretic chromatography.


CONCLUSIONS
Electrophoretic chromatography is a useful tool for the fractionation of schistosome antigens.
Yuan
Shi-shan
SS
Laboratory of Schistosomiasis, Xiangya School of Medicine, Central South University, Changsha, 410078, China.
Zeng
Xian-fang
XF
Huang
Yue-long
YL
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis / Q000302:isolation & purification*; D004586:Electrophoresis; D015801:Helminth Proteins / Q000032:analysis / Q000302:isolation & purification*; D011817:Rabbits; D012549:Schistosoma japonicum / Q000737:chemistry / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000469:parasitology
D000947:Antigens, Helminth; D015801:Helminth Proteins