Use of bioactive glass slides for matrix-assisted laser desorption/ionization analysis: application to microorganisms.
Glass slides are widely used in high-throughput analysis and are available commercially with surfaces activated, etched, and channeled. Thin glass microscope slides are shown here to be suitable sample supports for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. As a demonstration, lectins immobilized on glass slides with activated surfaces are used to concentrate and purify agglutinated Bacillis spores. It is expected that such slides will provide a rapid, inexpensive way to evaluate and implement new strategies involving MALDI MS readout.
Afonso
Carlos
C
Chemistry and Biochemistry Department, University of Maryland, College Park, Maryland 20742, USA. afonso@ccr.jussieu.fr
Fenselau
Catherine
C
D001409:Bacillus cereus / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D007834:Lasers; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D013171:Spores, Bacterial