Conversion of hexadecanoic acid to hexadecenoic acid by rat Delta 6-desaturase.
A higher content of C16:1 n-10 has recently been reported in the preputial gland of mice with a targeted disruption of the gene encoding stearoyl-CoA desaturase 1 (SCD1-/- mice) when compared with wild-type mice. This result has provided the first physiological evidence for the presence and regulation of a palmitoyl-CoA Delta 6-desaturase in mammals. To investigate the putative involvement of the known Delta 6-desaturase (FADS2) in this process, COS-7 cells expressing rat Delta 6-desaturase were incubated with C16:0. Transfected cells were able to synthesize C16:1 n-10, while nontransfected cells did not produce any C16:1 n-10. Evidence is therefore presented that the rat Delta 6-desaturase, which acts on the 18- and 24-carbon fatty acids of the n-6 and n-3 series, is also able to catalyze palmitic acid Delta 6 -desaturation.
Guillou
Hervé
H
Laboratoire de Biochimie, INRA-ENSA, Rennes, France.
Rioux
Vincent
V
Catheline
Daniel
D
Thibault
Jean-Nöel
JN
Bouriel
Monique
M
Jan
Sophie
S
D'Andrea
Sabine
S
Legrand
Philippe
P
D000818:Animals; D015153:Blotting, Western; D019556:COS Cells; D004591:Electrophoresis, Polyacrylamide Gel; D044943:Fatty Acid Desaturases / Q000235:genetics / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D018014:Gene Transfer Techniques; D019308:Palmitic Acid / Q000378:metabolism*; D010169:Palmitic Acids / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism*
D010169:Palmitic Acids; D011994:Recombinant Proteins; D019308:Palmitic Acid; C093821:hexadecenoic acid; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; C474452:delta 6-desaturase, rat