[Biological characteristics of mesenchymal stem cells in vitro derived from bone marrow of banna minipig inbred line].
OBJECTIVE
To isolate and characterize mesenchymal stem cells (MSCs) derived from bone marrow of Banna minipig inbred line (BMI).


METHODS
BMI-MSCs was isolated from bone marrow by density gradient centrifugation and cultured in DMEM (containing 15% bovine serum) at 37 degrees C with humidified 5% CO2. These cultured stem cells were characterized in clonal growth, expression of specific markers and capability of differentiation.


RESULTS
Mesenchymal stem cells were proliferative and could be expanded rapidly in vitro. Clonal growth of these cells can be observed when small amount of cells was inoculated. These cells were SH2, SH3, SH4, SB10 and SB21 positive. And it was proved that these cells possess osteo-differentiation ability, up-regulated alkaline phosphatase expression and calcium secretion after osteosupplement was added into the media for several days.


CONCLUSIONS
Mesenchymal stem cells derived from bone marrow of BMI possess the general characters of stem cell.
Li
Sheng-fu
SF
Key Laboratory of Transplant Engineering and Immunology, Ministry of Public Health, West China Hospital, Sichuan University, Chengdu, Sichuan, P. R. China 610041. lishengfu@163.net
Lu
Xiao-feng
XF
Sun
Ming-han
MH
D000818:Animals; D017849:Animals, Inbred Strains; D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D003470:Culture Media / Q000494:pharmacology; D008297:Male; D010006:Osteoblasts / Q000166:cytology; D013234:Stem Cells / Q000166:cytology*; D013556:Swine, Miniature
D003470:Culture Media