[Expression of human anti-apoptotic gene survivin and its splice in normal human gastric tissue and gastric cancer].
OBJECTIVE
To analyze the expression of human anti-apoptotic gene survivin (SVV) in normal human gastric tissues and gastric cancer.


METHODS
SVV cDNA clone was obtained from human gastric cancer tissues by virtue of RT-PCR, using Dig-marked cRNA probe in situ hybridization to analyze its expression in normal human gastric tissues and gastric cancer.


RESULTS
Two SVV cDNA clones, SVV-S4A and SVV-S1B were obtained. The sequence of the former is identical to that of the well-known SVV cDNA; however, in the sequence of the latter, the third exon was missed, i.e., there are only two exons in SVV-S1B. In situ hybridization showed that SVV-S4A is mainly expressed in gastric cancer tissues whereas SVV-S1B is mostly expressed in normal gastric tissues.


CONCLUSIONS
There is difference between SVV-S4A and SVV-S1B in respect to their characteristics of expression in gastric cancer and normal gastric tissues.
Yang
Xin-yan
XY
Department of Gastroenterology, General Hospital of Chinese PLA, Beijing, PR China. yangxy33@hotmail.com
Wang
Meng-wei
MW
Wang
Gang-shi
GS
You
Wei-di
WD
D017398:Alternative Splicing / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D017422:Sequence Analysis, DNA; D013274:Stomach Neoplasms / Q000235:genetics*; D000077022:Survivin
C107489:BIRC5 protein, human; D018076:DNA, Complementary; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D000077022:Survivin