title: Expression of organic anion transporting polypeptide E (OATP-E) in human placenta.
abstract: OBJECTIVE
  To examine the expression of multifunctional Na(+)-independent organic anion transporting polypeptide, termed OATP-E, involved in the transport of thyroid hormone in human placenta.
  
  
  METHODS
  Western blot analysis was performed using a specific antibody against OATP-E in human placenta to confirm the expression of OATP-E at the protein level. Immunohistochemistry was also performed using the specific antibody against OATP-E on frozen sections of human placenta.
  
  
  RESULTS
  By Western blot analysis, a single band for OATP-E was observed in human placenta. Immunohistochemistry revealed that OATP-E was predominantly expressed at the apical surface of the syncytiotrophoblasts in placenta.
  
  
  CONCLUSIONS
  These results reveal that OATP-E is expressed in human placenta, suggesting a functional role for the transplacental transfer of thyroid hormone.
authors.affiliation: Department of Obstetrics and Gynecology, Tohoku University Graduate School of Medicine, Sendai, 980-8574, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: J
authors.initials: J
authors.lastname: Sugawara
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Mizutamari
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: A
authors.initials: A
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Mikkaichi
authors.forename: T
authors.initials: T
authors.lastname: Onogawa
authors.forename: M
authors.initials: M
authors.lastname: Tanemoto
authors.forename: M
authors.initials: M
authors.lastname: Unno
authors.forename: T
authors.initials: T
authors.lastname: Abe
authors.forename: K
authors.initials: K
authors.lastname: Okamura
mesh_terms: D000328:Adult; D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D027361:Organic Anion Transporters / Q000378:metabolism*; D010446:Peptide Fragments / Q000276:immunology; D011247:Pregnancy; D011817:Rabbits; D013963:Thyroid Hormones / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*
chemical_list: D000906:Antibodies; D027361:Organic Anion Transporters; D010446:Peptide Fragments; D013963:Thyroid Hormones