[Expression of bcl-2 gene in human trabecular cells induced by dexamethasone].
OBJECTIVE
To investigate the influence of dexamethasone on expression of bcl-2 gene in human trabecular cells.


METHODS
The trabecular specimens from human eyes were cultured in vitro and the cultured cells of the third generation on slides were immersed in culture solution containing 10(-7) M dexamethasone for 6 hours, 12 hours and 24 hours respectively. The expression of bcl-2 gene was studied with LSAB.


RESULTS
The positive cells of the bcl-2 protein could be identified after dexamethasone effected for 6 hours and the positive cell increased with time going on.


CONCLUSIONS
The expression of bcl-2 gene can be induced by dexamethasone, it may be related with glaucomatic pathogenesis.
Zhuo
Y
Y
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Ge
J
J
Lin
M
M
Zheng
J
J
Li
Y
Y
D017209:Apoptosis; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D005901:Glaucoma / Q000139:chemically induced; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D014129:Trabecular Meshwork / Q000166:cytology / Q000378:metabolism*
D019253:Proto-Oncogene Proteins c-bcl-2; D003907:Dexamethasone