[Effect of xanthone from Canscora lucidissima on cultured myocytes anoxia-reoxygenation injuries].
The anoxia/reoxygenation model of cultured neonatal rats myocardial cells were developed, according to Laarse's method: anoxia and glucose deficiency for 60 min followed by reoxygenation and re-exposure to glucose for 30 min. The results showed that the survival rate of myocardial cells in the anoxia group was significantly decreased, release of lactate dehydrogenase (LDH) from myocytes was increased and membrane fluidity was decreased, all the changes were much more severe in the reoxygenation group. 30 min before anoxia, addition of xanthones: 1,8-dihydroxy-3, 5-dimethoxyxanthone, 1-hydroxy-3,5-dimethoxyanthone and 1-hydroxy-3,7,8-trithoxyxanthone, isolated from Canscora lucidissima increased the rate of myocardial cells and membrane fluidity, decreased the release of LDH. These results suggested that xanthone may provide some protective effects on the anoxia/reoxygenation damages on myocardium.
He
Q
Q
Guangzhou Research Institute of Snake Venom, Guangzhou 510182.
He
L
L
Xu
S
S
Deng
Q
Q
D000818:Animals; D015687:Cell Hypoxia; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014966:Xanthenes / Q000302:isolation & purification / Q000494:pharmacology*; D044004:Xanthones
D014966:Xanthenes; D044004:Xanthones; C009689:xanthone; D007770:L-Lactate Dehydrogenase