Urinary heat shock protein-72 excretion in clinical and experimental renal ischemia.
Renal ischemia not only causes injury but also induces repair mechanisms, such as the cellular induction of the 72-kilodalton heat shock protein HSP-72. The aim of this study was to determine whether HSP-72 is excreted in urine after ischemic renal injury. The first urine of six pediatric allograft recipients was examined for proteinuria and urinary HSP-72 excretion. Sprague-Dawley rats were treated with renal ischemia or hyperthermia and renal cortex and urinary HSP-72 levels were determined. HSP-72 was excreted in the first urine of renal allografts. In rats, renal HSP-72 was induced both by renal ischemia or hyperthermia. However, only renal ischemia resulted in urinary excretion of HSP-72. Urinary excretion of HSP-72 indicates an increased renal stress response and loss of tubular cell integrity after clinical and experimental renal ischemia.
Mueller
Thomas
T
Department of Pediatrics, Kinderdialyse, AKH Wien, Waehringer Guertel 18-20, 1090 Vienna, Austria.
Bidmon
Bettina
B
Pichler
Patrick
P
Arbeiter
Klaus
K
Ruffingshofer
Dagmar
D
VanWhy
Scott K
SK
Aufricht
Christoph
C
D000818:Animals; D002648:Child; D002675:Child, Preschool; D005260:Female; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000652:urine*; D006801:Humans; D007511:Ischemia / Q000652:urine*; D007668:Kidney / Q000098:blood supply*; D016030:Kidney Transplantation; D008297:Male; D011507:Proteinuria / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D014184:Transplantation, Homologous
D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins