[Antifibrotic effects of genistein and quercetin in vitro].
OBJECTIVE
To study the antifibrotic effects of genistein (GE) and quercetin (QU) on rat hepatic stellate HSC-T6 cell proliferation stimulated with platelet-derived growth factor (PDGF), collagen synthesis and type I procollagen messenger RNA (mRNA) expression stimulated with transforming growth factor beta 1 (TGF beta 1).


METHODS
Cell proliferation was measured by crystal violet staining assay. Collagen synthesis was determined by 3H-proline incorporation assay. Type I procollagen mRNA level was determined by reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
GE (25-70 mumol.L-1) and QU (6.25-50 mumol.L-1) concentration-dependently attenuated PDGF-drive HSC-T6 cell proliferative activity. TGF beta 1-stimulated collagen synthesis was also reduced. This was associated with a decrease of type I procollagen mRNA, indicating an effect at a pretranslational level.


CONCLUSIONS
GE and QU may have therapeutic potential against liver fibrosis by regulating PDGF and TGF beta 1 actions.
Qi
L H
LH
Department of Pharmacy, Fuzhou General Hospital of Nanjing Command, Fuzhou 350001, China.
Kang
L P
LP
Zhang
J P
JP
Shi
N
N
Zhang
M
M
Wu
T M
TM
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D019833:Genistein / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors; D011794:Quercetin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors; D053773:Transforming Growth Factor beta1
D000975:Antioxidants; D024042:Collagen Type I; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D003094:Collagen; D011794:Quercetin; D019833:Genistein