Native Myosin-IXb is a plus-, not a minus-end-directed motor.
Myosin-IXb (Myo9b) is a single-headed, processive motor that contains a Rho-GTPase-activating protein (GAP) domain within its tail. Although tail-less myosin- IXb motor domain moves towards the minus end of the actin filament, we show here that full-length myosin-IXb is a plus-end-directed motor. This suggests that the tail domain of myosin-IXb regulates motor directionality.
O'Connell
Christopher B
CB
Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06511, USA.
Mooseker
Mark S
MS
D000199:Actins / Q000378:metabolism; D000906:Antibodies / Q000378:metabolism; D020409:Molecular Motor Proteins / Q000378:metabolism*; D024701:Myosin Type V / Q000378:metabolism; D009218:Myosins / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D013997:Time Factors
D000199:Actins; D000906:Antibodies; D020409:Molecular Motor Proteins; C113469:myosin IXB; D024701:Myosin Type V; D009218:Myosins