Novel cell culture models for prevention of human breast cancer (Review).
Human breast cancer is a multifactorial, multistep disease wherein genetic, endocrine and dietary factors represent crucial regulators of initiation, promotion and progression. Preclinical investigations utilizing human breast carcinoma derived cell lines either in culture, or upon xenotransplantation, have provided valuable leads for molecular pathogenesis of cancer progression and also for novel therapeutic modalities. The mechanistic significance of genetic factors on early events of initiation/promotion, however, is dependent on extrapolation, and is therefore, equivocal. Human tissue derived explant culture/cell culture models utilizing non-involved target tissue at risk for carcinogenic transformation provide a novel approach that minimizes extrapolation for clinical relevance and thereby maximizes the translational impact. This report provides an overview of laboratory investigations focused on: i) development of the model, ii) optimization of mechanistic biomarker assays for carcinogenic transformation, and iii) validation of the model as a high throughput mechanistic screen for preclinical efficacy of natural phytochemicals.
Katdare
Meena
M
Carcinogenesis and Chemoprevention Research Laboratory, Strang Cancer Prevention Center, and Department of Surgery, Weill Medical College of Cornell University, New York, NY, USA.
Osborne
Michael P
MP
Telang
Nitin T
NT
D016588:Anticarcinogenic Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D015415:Biomarkers; D001940:Breast / Q000166:cytology / Q000187:drug effects; D001943:Breast Neoplasms / Q000473:pathology / Q000517:prevention & control*; D044584:Carcinoma, Ductal / Q000473:pathology / Q000517:prevention & control*; D002453:Cell Cycle / Q000187:drug effects; D018797:Cell Cycle Proteins / Q000502:physiology; D045744:Cell Line, Tumor / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D004354:Drug Screening Assays, Antitumor; D004847:Epithelial Cells / Q000187:drug effects; D005260:Female; D006801:Humans; D010945:Plants, Edible / Q000737:chemistry
D016588:Anticarcinogenic Agents; D015415:Biomarkers; D018797:Cell Cycle Proteins