[Comparison of the immune response to gene transfer into the rat brain with helper or helper virus-free HSV-1 vectors].
OBJECTIVE
This study was conducted to compare the immune response to gene transfer with helper virus or helper virus-free vectors.


METHODS
Two vector stocks were delivered by stereotactic injection into the striatum and then the immune response markers including TCR, CD4, CD8, MHC-I and MHC-II were examined.


RESULTS
The data showed that the immune response to either vector stock reached maximal levels by 4 to 7 days after gene transfer. However, the maximal levels of all markers to the helper virus-free stock were 3 to 10 folds lower than the markers to the helper virus stock. The immune response to the helper virus-free stock declined rapidly to be close to the levels of PBS control.


CONCLUSIONS
The immune response to the helper virus-free stock is significantly less than the response to the vector stock that contains helper virus.
Zhang
Wei
W
Department of Histology and Embryology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Geller
A
A
D000818:Animals; D015704:CD4 Antigens / Q000276:immunology*; D016827:CD8 Antigens / Q000276:immunology; D003342:Corpus Striatum / Q000378:metabolism*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006378:Helper Viruses / Q000235:genetics* / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000378:metabolism; D007109:Immunity; D008285:Major Histocompatibility Complex / Q000276:immunology; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*
D015704:CD4 Antigens; D016827:CD8 Antigens; D011948:Receptors, Antigen, T-Cell