title: Analysis of strigolactones, germination stimulants for striga and orobanche, by high-performance liquid chromatography/tandem mass spectrometry.
abstract: A simple and rapid analytical method for strigolactones, germination stimulants for the root parasitic weeds witchweed (Striga spp.) and broomrape (Orobanche spp.), has been developed using high-performance liquid chromatography connected to tandem mass spectrometry (LC/MS/MS). The natural strigolactones (strigol, sorgolactone, orobanchol, and alectrol) were clearly separated and identified by LC/MS/MS. As low as 0.1 pg/microL of strigol and 0.5 pg/microL of sorgolactone could be quantified, whereas 1 pg/microL was needed for the quantification of orobanchol (S/N > 10). Using this method, it was found that red clover produces orobanchol and alectrol but not strigol. The roots of red clover seedlings were found to produce 13, 70, 58, and 65 pg of orobanchol/plant 1, 2, 3, and 4 weeks after germination, respectively.
authors.affiliation: Center for Research on Wild Plants, Utsunomiya University, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sato
authors.forename: Ayman A
authors.initials: AA
authors.lastname: Awad
authors.forename: Sang Heon
authors.initials: SH
authors.lastname: Chae
authors.forename: Takao
authors.initials: T
authors.lastname: Yokota
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Sugimoto
authors.forename: Yasutomo
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: Koichi
authors.initials: K
authors.lastname: Yoneyama
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018525:Germination; D007783:Lactones / Q000032:analysis* / Q000097:blood; D031672:Orobanche / Q000737:chemistry* / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D034362:Striga / Q000737:chemistry* / Q000378:metabolism
chemical_list: D007783:Lactones; C472679:alectrol; C472678:orobanchol