[The effect of interleukin-1 beta on cell growth of normal mouse pituitary cells and ACTH secreting adenoma].
OBJECTIVE
To investigate whether IL-1 beta could affect the cell proliferation of normal pituitary cells and ACTH secreting adenoma cells.


METHODS
Normal mouse pituitary cells were cultured in mininum essential medium-D-valine medium, a cultures virtually free of fibroblasts. AtT20 cells were also cultured as an ACTH secreting adenoma model. MTT method was used to study 10(-9) mol/L-10(-13) mol/L IL-1 beta effect on both normal and adenoma cells growth.


RESULTS
(1) In the primate cells culture of normal mouse pituitary, IL-1 beta failed to influence cell growth during a period of 72 hours (n = 9, P > 0.05). This finding can not confirm that IL-1 beta has no effect on pituitary cell growth, because of the variety of cell types in pituitary. (2) In the AtT20 cells, 10(-9) mol/L-10(-13) mol/L IL-1 beta stimulated cell proliferation significantly after 48 and 72 hours culturing respectively (n = 9, P < 0.05, P < 0.01).


CONCLUSIONS
These results suggested that IL-1 beta might be partially in involved in modulating pituitary adenoma cells growth.
Guo
A
A
Department of Endocrinology, PUMC Hospital, CAMS and PUMC, Beijing 100730.
Wang
Z
Z
Lu
Z
Z
D000236:Adenoma / Q000473:pathology*; D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010902:Pituitary Gland / Q000166:cytology*; D010911:Pituitary Neoplasms / Q000473:pathology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D007375:Interleukin-1; D011994:Recombinant Proteins; D000324:Adrenocorticotropic Hormone