title: Molecular cloning of endo-beta-D-1,4-glucanase genes, rce1, rce2, and rce3, from Rhizopus oryzae.
abstract: Three endoglucanase genes, designated the rce1, rce2, and rce3 genes, were isolated from Rhizopus oryzae as the first cellulase genes from the subdivision ZYGOMYCOTA: All the amino acid sequences deduced from the rce1, rce2, and rce3 genes consisted of three distinct domains: cellulose binding domains, linker domains, and catalytic domains belonging to glycosyl hydrolase family 45. The rce3 gene had two tandem repeated sequences of cellulose binding domains, while rce1 and rce2 had only one. rce1, rce2, and rce3 had various lengths of linker sequences.
authors.affiliation: Microbiological Resources and Technology Laboratories, Meiji Seika Kaisha, Ltd., Saitama 350-0289, Japan.
authors.forename: Tatsuki
authors.initials: T
authors.lastname: Moriya
authors.forename: Koichiro
authors.initials: K
authors.lastname: Murashima
authors.forename: Akitaka
authors.initials: A
authors.lastname: Nakane
authors.forename: Koji
authors.initials: K
authors.lastname: Yanai
authors.forename: Naomi
authors.initials: N
authors.lastname: Sumida
authors.forename: Jinichiro
authors.initials: J
authors.lastname: Koga
authors.forename: Takeshi
authors.initials: T
authors.lastname: Murakami
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kono
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D020134:Catalytic Domain; D002480:Cellulase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006031:Glycosylation; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012233:Rhizopus / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D002480:Cellulase