[Studies on immunological reaction of the antiserum of recombinant secreted protein from Ancylostoma caninum].
OBJECTIVE
To analyze the immunological reaction of the antiserum of recombinant secreted protein from Ancylostoma caninum with antigens of various species hookworms at different developmental stages.


METHODS
SDS-PAGE and ELIB technique were employed in the study.


CONCLUSIONS
The protein component of Ac-rAsp-1 was 45 kDa, its immune serum can recognize the antigens of Ac-L3 and Ac-rAsp-1 protein, but not react to the antigens of Ad-A, Ad-L3, Na-A, Ac-A, Nb-A and Ac-rAsp-2 protein. The protein component of Ac-rAsp-2 was 24 kDa, its immune serum can recognize the antigens of Ad-A, Ad-L3, Na-A, Ac-A, Ac-L3 and Ac-rAsp-2 protein, but not react to the antigens of Nb-A and Ac-rAsp-1 protein.
Wen
L Y
LY
Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou 310013.
Hotez
P J
PJ
D000722:Ancylostoma / Q000276:immunology*; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D004285:Dogs; D015801:Helminth Proteins / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000276:immunology
C099548:ASP protein, Ancylostoma caninum; D000947:Antigens, Helminth; D015801:Helminth Proteins; D007106:Immune Sera; D011994:Recombinant Proteins