title: Protection against damaged DNA in the single cell by polyphenols.
abstract: The protective properties of seven polyphenols against hydrogen peroxide induced DNA damage in human peripheral blood lymphocytes (PBL) were studied using single cell micro-gel electrophoresis. Hydrogen peroxide causes a concentration-dependent increase in single cell DNA strand breakage in human PBL. Quercetin and 7,8-dihydroxy-4-methyl coumarin exhibited the strongest protection, significantly inhibiting 50 microM H2O2-induced DNA damage at a range of concentrations of 3.1-25 microM. Curcumin, resveratrol and vanillin protected against DNA damage induced by 50 microM H2O2 at a range of concentrations of 6.25-25 microM, but rutin and 7-hydroxy-4-methyl coumarin failed to provide any protection even at concentrations up to 50 microM. Quercetin, 7,8-dihydroxy-4-methyl coumarin, curcumin, resveratrol and vanillin are therefore effective in protection of human single cell DNA from oxidative attack.
authors.affiliation: School of Life Sciences, Lanzhou University, Lanzhou, People's Republic of China.
authors.forename: Guo-An
authors.initials: GA
authors.lastname: Liu
authors.forename: Rong-Liang
authors.initials: RL
authors.lastname: Zheng
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004586:Electrophoresis; D005260:Female; D005419:Flavonoids; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008214:Lymphocytes / Q000187:drug effects; D008856:Microscopy, Fluorescence; D016877:Oxidants / Q000633:toxicity; D010636:Phenols / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D059808:Polyphenols; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D007202:Indicators and Reagents; D016877:Oxidants; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D006861:Hydrogen Peroxide