title: [Complete Freund's adjuvant-induced splenic T cell apoptosis in the prevention of diabetes in non-obese diabetic mice].
abstract: OBJECTIVE
  To study the apoptosis of T lymphocytes in the spleen and its role in complete Freund's adjuvant (CFA) preventing diabetes in non-obese diabetic (NOD) mice.
  
  
  METHODS
  Forty-two NOD mice were divided into two groups randomly: CFA-treated NOD female mice (6w, 12w and 30w) and PBS-treated NOD female mice (6w, 12w and 30w). The apoptosis of splenic T lymphocytes was observed with the TDT-mediated fluorescein-dUTP nick-end labelling (TUNEL) technique and avidin-biotin complex method.
  
  
  RESULTS
  CFA induced the apoptosis of CD4+ T lymphocytes in the mouse spleen and could prevent the development of Type 1 diabetes (T1DM) in NOD female mice.
  
  
  CONCLUSIONS
  CFA preventing Type 1 diabetes in NOD mice may be related to the apoptosis of T lymphocytes in the spleen, which downregulates mature CD4+ T lymphocytes in the periphery.
authors.affiliation: Department of Endocrinology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Xiao-xi
authors.initials: XX
authors.lastname: Wang
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Niu
authors.forename: Zhi-guang
authors.initials: ZG
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology*; D051379:Mice; D016688:Mice, Inbred NOD; D011897:Random Allocation; D013154:Spleen / Q000166:cytology
chemical_list: D005620:Freund's Adjuvant