Indirect fluorescent antibody testing of nasopharyngeal swabs for influenza diagnosis in lung transplant recipients.
BACKGROUND
Rapid and reliable diagnosis of respiratory viral infections (RVI) in lung transplant recipients is essential to direct therapy of acute graft dysfunction and identify epidemic trends. Traditional techniques of serology and viral culture are limited by the lack of antibody response and delay in diagnosis.


METHODS
We examined the clinical utility of indirect fluorescent antibody (IFA) testing in adult lung transplant patients with suspected RVI, compared with serology and culture. Nasopharyngeal and throat swabs (NT) were obtained to sample epithelial cells, followed by application of monoclonal antibody to respiratory syncytial virus, adenovirus, parainfluenza 1-3 and influenza A and B. The Bartels Respiratory Viral Detection kit was used with IFA results available within 24 hours.


RESULTS
Nine of 18 patients tested positive for RVI with influenza A (n = 8) and influenza B (n = 1) detected. The sensitivity of IFA (67%) was higher than that of cell culture (45%). With intensive supportive therapy, infection was self-limiting in bronchiolitis obliterans syndrome (BOS) Grade 0-2 patients. However, patients with BOS Grade 3 manifested an acute exacerbation of airflow obstruction, which proved to be irreversible.


CONCLUSIONS
Lung transplant patients with "flu-like" symptoms should proceed to IFA testing of NT swab specimens for early diagnosis. Samples collected within 7 days of symptom onset have high sensitivity as compared with serology and viral culture techniques.
Hopkins
Peter M
PM
Heart Lung Transplant Unit, St Vincent's Hospital, Darlinghurst, Sydney, Australia. peterawkatipu@hotmail.com
Plit
Marshall L
ML
Carter
Ian W
IW
Chhajed
Prashant N
PN
Malouf
Monique A
MA
Glanville
Allan R
AR
D000293:Adolescent; D000328:Adult; D001989:Bronchiolitis Obliterans / Q000821:virology; D016022:Case-Control Studies; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D009980:Influenza A virus / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis*; D016040:Lung Transplantation; D008297:Male; D008875:Middle Aged; D009305:Nasopharynx / Q000821:virology*; D010614:Pharynx / Q000821:virology; D011446:Prospective Studies; D012680:Sensitivity and Specificity