title: Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate.
abstract: (-)-Epigallocatechin gallate (EGCG), abundant in green tea, is a potent anti-microbial and anti-tumor compound. This investigation used immunoblot, flow cytometry, microarray, and indirect immunofluorescence analyses to show that at concentrations exceeding 50 microM, EGCG inhibits the expression of Epstein-Barr virus (EBV) lytic proteins, including Rta, Zta, and EA-D, but does not affect the expression of EBNA-1. Moreover, DNA microarray and transient transfection analyses demonstrated that EGCG blocks EBV lytic cycle by inhibiting the transcription of immediate-early genes, thus inhibiting the initiation of EBV lytic cascade.
authors.affiliation: Department of Biology, Kaohsiung Medical University, 100, Shih-Chuan 1st Rd., 807, Kaohsiung, Taiwan.
authors.forename: Li-Kwan
authors.initials: LK
authors.lastname: Chang
authors.forename: Ta-Tung
authors.initials: TT
authors.lastname: Wei
authors.forename: Ya-Fang
authors.initials: YF
authors.lastname: Chiu
authors.forename: Chao-Ping
authors.initials: CP
authors.lastname: Tung
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Chuang
authors.forename: Shang-Kai
authors.initials: SK
authors.lastname: Hung
authors.forename: Ching
authors.initials: C
authors.lastname: Li
authors.forename: Shih-Tung
authors.initials: ST
authors.lastname: Liu
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000096:biosynthesis / Q000235:genetics; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D017781:Genes, Immediate-Early / Q000187:drug effects; D004854:Herpesvirus 4, Human / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000998:Antiviral Agents; D019309:Epstein-Barr Virus Nuclear Antigens; D014764:Viral Proteins; D002392:Catechin; C045651:epigallocatechin gallate; C417029:EBV-encoded nuclear antigen 1