title: Immunohistochemical analysis for histopathological subtypes in pediatric medulloblastomas.
abstract: Medulloblastomas occurring in children represent a histological spectrum of varying anaplasia and nodularity. In order to determine whether immunohistochemical markers might be useful parameters in subclassifying these tumors, 17 pediatric medulloblastomas, including nine diffuse/non-anaplastic, four diffuse/anaplastic, three nodular/non-anaplastic and one nodular/anaplastic subtypes, were studied. In the present report, we investigate the expression of neural cell adhesion molecule (NCAM), nerve growth factor receptor (NGFR), neurofilament (NF), synaptophysin (SYN), glial fibrillary acidic protein (GFAP), S100, Bcl-2, and Ki-67 by using the immunohistochemistry against specific antibodies. This study showed that NGFR, NF, GFAP and S100 were not detected in anaplastic subtypes of medulloblastomas (0/5), while non-anaplastic subtypes were mainly expressed within the nodules. All 17 tumors were reactive for NCAM, SYN and Bcl-2. In addition, Ki-67 labeling indices for anaplastic subtypes (39.0 +/- 7.42%) were significantly higher than that of non-anaplastic medulloblastomas (11.4 +/- 8.04%; P < 0.0001). These results suggest that immunohistochemical markers are a useful adjunct in characterizing subtypes of pediatric medulloblastomas.
authors.affiliation: Department of Neurosurgery, Institute for Medical Science, Keimyung University School of Medicine, Deagu, Korea.
authors.forename: Eun-Ik
authors.initials: EI
authors.lastname: Son
authors.forename: Il-Man
authors.initials: IM
authors.lastname: Kim
authors.forename: Dong-Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Man Bin
authors.initials: MB
authors.lastname: Yim
authors.forename: Yu-Na
authors.initials: YN
authors.lastname: Kang
authors.forename: Sang-Sook
authors.initials: SS
authors.lastname: Lee
authors.forename: Kun-Young
authors.initials: KY
authors.lastname: Kwon
authors.forename: Seong-Il
authors.initials: SI
authors.lastname: Suh
authors.forename: Taeg-Kyu
authors.initials: TK
authors.lastname: Kwon
authors.forename: Jung-Jeung
authors.initials: JJ
authors.lastname: Lee
authors.forename: Dong-Sug
authors.initials: DS
authors.lastname: Kim
authors.forename: Sang-Pyo
authors.initials: SP
authors.lastname: Kim
mesh_terms: D000293:Adolescent; D014408:Biomarkers, Tumor / Q000378:metabolism; D002528:Cerebellar Neoplasms / Q000145:classification* / Q000378:metabolism / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008527:Medulloblastoma / Q000145:classification* / Q000378:metabolism / Q000473:pathology*; D009363:Neoplasm Proteins / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins