Electrophysiological response of cultured trabecular meshwork cells to synthetic ion channels.
The response of living cells of the trabecular meshwork to synthetic ion channels is described. The THF-gramicidin hybrids THF-gram and THF-gram-TBDPS as well as a linked gA-TBDPS and gramicidin A were applied to cultured ocular trabecular meshwork cells. THF-gram application (minimal concentration, 10(-8) M; saturation, 10(-7) M) led to an additional conductance which displayed characteristics of weak Eisenman-I-selective cation channels, no cell destruction, an asymmetric change of the inward/outward currents, and higher current densities using Cs(+) as charge carrier compared to Na(+) and K(+). Linked-gA-TBDPS showed at 10(-12) M increases of the membrane conductance comparable to gA at 10(-7) M and a much faster response of the cells. Thus, THF-gramicidin hybrids form a basis for the use of synthetic ion channels in biological systems, which eventually may lead to new therapeutic approaches.
Fidzinski
Pawel
P
Institut für Klinische Physiologie, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Hindenburgdamm 30, 12200 Berlin, Germany.
Knoll
Andrea
A
Rosenthal
Rita
R
Schrey
Anna
A
Vescovi
Andrea
A
Koert
Ulrich
U
Wiederholt
Michael
M
Strauss
Olaf
O
D000818:Animals; D002417:Cattle; D002586:Cesium / Q000378:metabolism; D004553:Electric Conductivity; D004594:Electrophysiology; D005663:Furans; D006096:Gramicidin / Q000737:chemistry / Q000494:pharmacology; D007473:Ion Channels / Q000138:chemical synthesis* / Q000494:pharmacology*; D017646:Organosilicon Compounds; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013329:Structure-Activity Relationship; D014129:Trabecular Meshwork / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology
D005663:Furans; D007473:Ion Channels; D017646:Organosilicon Compounds; D006096:Gramicidin; D002586:Cesium; C018674:tetrahydrofuran; D012964:Sodium; D011188:Potassium