Genetic variation in the promoter region of the basic fibroblast growth factor gene.
Basic fibroblast growth factor (bFGF) is a member of the fibroblast growth factor family that possess broad mitogenic and cell survival activities and is involved in a variety of biological processes. We investigated possible genetic polymorphism in the promoter and 5' flanking region of the bFGF gene. Polymorphism was analysed by means of heteroduplex analysis, fragments with altered mobility were sequenced. Three novel substitutions (-553T/A, -834T/A and -921C/G) were identified in the promoter region. Allele frequencies in a sample of healthy Caucasian subjects (n=126) were determined by polymerase chain reactions followed by restriction analyses with specific endonucleases. The frequencies of the mutated alleles (-553A, -834A and -921G) were 0.04, 0.05 and 0.14, respectively. Newly identified variants in the bFGF gene promoter appear to be common polymorphisms in the Czech population.
Beránek
Michal
M
Centre of Biostatistics and Analyses, Brno, Czech Republic. beranek@sci.muni.cz
Tschöplová
Svatava
S
Kanková
Katerina
K
Kuhrová
Vera
V
Vácha
Jirí
J
D024506:5' Flanking Region; D000368:Aged; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D011401:Promoter Regions, Genetic
D016222:Fibroblast Growth Factor 2