[The effect of noise exposure on the expression of NOSmRNA in the cochlea].
OBJECTIVE
To study the effect of NO in noise-induced deafness.


METHODS
The effect of noise exposure on the expression of NOSmRNA in the guinea pig cochlea was observed with NADPH-diaphorase histochemistry staining, NOSmRNA hybridization in situ and Northern blotting analysis.


RESULTS
NADPH-diaphorase histochemistry indicated that NOS mainly presents in the inner hair cells, outer hair cells of Corti's organ, spiral ganglion cells and the marginal cells of stria vascularis in the guinea pig cochlea; NOSmRNA hybridization in situ showed that there is positive signal in the inner hair cells, outer hair cells and spiral ganglion cells, but there is no positive signal in the stria marginal cells of vascularis; Northern blotting showed that the expression of noise exposure group is more intensive than that of the control group.


CONCLUSIONS
There is a Glu: NMDA receptor, NO: cGMP pathway in the cochlear afferent possibly; noise exposure induced overproduction of NO mediates the effect of neurotoxicity; the marginal cells of stria vascular produce and release NO to regulate the microcirculation of the cochlea.
Sun
D Y
DY
Department of ENT, Third Hospital, Beijing Medical University, Beijing 100083.
Li
X P
XP
D000818:Animals; D003051:Cochlea / Q000201:enzymology*; D005260:Female; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000201:enzymology*; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D009622:Noise; D012333:RNA, Messenger / Q000096:biosynthesis
D012333:RNA, Messenger; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase