[Morphological changes of cultured rabbit retinal cells after injury].
OBJECTIVE
To investigate the morphological changes of cultured rabbit retinal cells after injury.


METHODS
Mechanical scratch was done in nenatol rabbit retinal cell culture and in Schwann Cell(SC)/retinal cell colculture. The morphological changes of different sorts of cells after the injury were observed under the phase contrast microscope for 24 h.


RESULTS
About 30 min after injury, many glial cells moved to the blank region and proliferated to form gliosis. Axons of retinal neurons began to regenerate 6 h post injury. The direction of regrowth was influenced by the environment. In the coculture, regeneration of axons was discerned only 3 h after injury.


CONCLUSIONS
Axons of retinal neurons had strong capability of regeneration; Gliosis was not the absolute obstacle of regeneration; SC played a role in promoting the axon regeneration.
Cai
L
L
Dept. of Ophthalmology, Xijing Hospital, 4th Military Medical University, XiAn 710032, China.
Hui
Y
Y
Meng
J
J
Yang
R
R
D000818:Animals; D000831:Animals, Newborn; D001369:Axons; D002478:Cells, Cultured; D018920:Coculture Techniques; D005131:Eye Injuries / Q000473:pathology*; D005911:Gliosis / Q000473:pathology; D008853:Microscopy; D009416:Nerve Regeneration; D009457:Neuroglia / Q000473:pathology; D009474:Neurons / Q000473:pathology; D011817:Rabbits; D012160:Retina / Q000293:injuries / Q000473:pathology*; D012583:Schwann Cells / Q000473:pathology*