Separation of conjugated trienoic fatty acid isomers by capillary electrophoresis.
A method for direct resolution of conjugated trienoic fatty acid isomers by capillary electrophoresis has been developed. To obtain complete separation a dual cyclodextrin system was used. This contained heptakis-(6-sulfo)-beta-cyclodextrin (charged). Beta-cyclodextrin (uncharged) and sodium dodecylsulfate. Under optimized conditions, all seven isomers were well separated. On average, separation efficiency was 2.9 x 10(5) plates/m.
Bohlin
Maria E
ME
Department of Chemistry, Karlstad University, SE-651 88 Karlstad, Sweden.
Ohman
Marcus
M
Hamberg
Mats
M
Blomberg
Lars G
LG
D019075:Electrophoresis, Capillary / Q000379:methods*; D005227:Fatty Acids / Q000737:chemistry / Q000302:isolation & purification*; D007536:Isomerism; D012967:Sodium Dodecyl Sulfate
D005227:Fatty Acids; D012967:Sodium Dodecyl Sulfate