title: [Extraction of RNA from Stored Bone Marrow Smears for Detecting Expression of the WT1 Gene in MDS Patients]
abstract: RNA was extracted from stored bone marrow smears, expression of WT1 gene in the patients with MDS was examined by means of nest RT-PCR. The results showed WT1 gene was highly expressed in some cases of MDS. Expression rates were higher in the patients with RAEB and RAEB-t than those patients with RA and RAS. WT1 expression is related to the advance of MDS. The detection of WT1 gene expression by RT-PCR might be useful for assessing disease progress in the patients with MDS.
authors.affiliation: Department of Hematology, Medical College, Jinan University, Guangzhou 510632, China; Ruian People's Hospitol, Zhejiang Province, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Xi-Yan
authors.initials: XY
authors.lastname: Po
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yu
authors.forename: Zhi-Shong
authors.initials: ZS
authors.lastname: Chen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhi-Fei
authors.initials: ZF
authors.lastname: Yu