[In vitro culture of human hair follicles and sequential observation].
OBJECTIVE
To obtain successful in vitro culture of human hair follicles, and observe their morphological changes in the middle and later period of culture.


METHODS
Human hair follicles were isolated from the scalp resected from patients receiving surgical treatment of the wrinkles, and were cultured in DMEM supplemented with 10% calf serum for up to 30 d. The growth of the follicles, along with their morphological and histological changes, was observed sequentially.


RESULTS
In vitro culture of human hair follicles was successfully obtained. The follicles showed catagen-like changes in the middle and later period of culture.


CONCLUSIONS
Human hair follicles cultured in vitro tend to transform early into catagen, and this culture method has simplified preparation of routine paraffin section of hair follicles.
Yan
Xin
X
Department of Plastic Surgery, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. yenshin@fimmu.com
Hu
Zhi-qi
ZQ
D000328:Adult; D048429:Cell Size; D002478:Cells, Cultured; D005260:Female; D018859:Hair Follicle / Q000166:cytology* / Q000254:growth & development; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged