G protein-coupled receptor overexpression with the baculovirus-insect cell system: a tool for structural and functional studies.
G protein-coupled receptors, whose topology shows seven transmembrane domains, form the largest known family of receptors involved in higher organism signal transduction. These receptors are generally of low natural abundance and overexpression is usually a prerequisite to their structural or functional characterisation. The baculovirus-insect cell system constitutes a versatile tool for the maximal production of receptors. This heterologous expression system also provides interesting alternatives for receptor functional studies in a well-controlled cellular context.
Massotte
Dominique
D
Laboratoire de Biologie et Génomique Structurales, UMR 7104, IGBMC, 1 rue Laurent Fries, BP 10142, F-67404 Illkirch Cedex, France. massotte@igbmc.u-strasbg.fr
D000818:Animals; D016367:Baculoviridae / Q000378:metabolism*; D002460:Cell Line; D003470:Culture Media; D018076:DNA, Complementary / Q000302:isolation & purification; D005786:Gene Expression Regulation; D020962:Heterotrimeric GTP-Binding Proteins / Q000737:chemistry; D006801:Humans; D007313:Insecta; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D015398:Signal Transduction
D003470:Culture Media; D018076:DNA, Complementary; C109334:GPR1 protein, S cerevisiae; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D020962:Heterotrimeric GTP-Binding Proteins