Relationship between plasmatic levels of various cytokines, tumour necrosis factor, enzymes, glucose and venom concentration following Tityus scorpion sting.
A sandwich enzyme-linked immunosorbent assay was developed for measuring Tityus venom levels in plasma. The method proved capable of distinguishing patients with only local symptoms from controls, and was used to quantify venom in 205 accidental human envenomations. Our results show that the severity of envenoming is related to the patient plasma venom concentration. This depends on time elapsed between the sting and when the plasma was drawn. We observed that 46 and 49% of patients with moderate to severe symptoms (MS, n=41) showed hyperamylasemia and hyperglycemia, respectively. In addition, 39% of cases with MS symptoms had partial thromboplastin time values prolonged or shorted and 6.5% of patients with local symptoms (LS, n=164) had only prolonged prothrombin time values. Interleukin 6 (IL6) increased significantly in patients with MS symptoms. IL6 values increased with hyperamylasemia, envenoming severity and time hyperamylasemia.
D'Suze
G
G
Lab de Neurofarmacología Celular, Biochemistry and Biophysics Center, Instituto Venezolano de Investigaciones Científicas (IVIC), Apdo 21827, Caracas 1020-A, Venezuela. gdsuze@ivic.ve
Moncada
S
S
González
C
C
Sevcik
C
C
Aguilar
V
V
Alagón
A
A
D000681:Amylases / Q000097:blood*; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D016207:Cytokines / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006943:Hyperglycemia / Q000097:blood* / Q000209:etiology; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D065008:Scorpion Stings / Q000097:blood* / Q000150:complications; D012604:Scorpion Venoms / Q000097:blood*; D012605:Scorpions; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D001786:Blood Glucose; D016207:Cytokines; D012604:Scorpion Venoms; D014409:Tumor Necrosis Factor-alpha; D000681:Amylases