[Molecular variation based on mtDNA-COII gene of members of the Anopheles minimus group].
OBJECTIVE
To investigate intra- and inter-species molecular variations and phylogeny of the five members of the Minimus Group of Anopheles subgenus Cellia in China: An. aconitus, An. varuna, An. jeyporiensis, An. minimus A and An. minimus C, and to report the DNA sequence divergence among these species at a mitochondrial locus (cytochrome oxidase II).


METHODS
Single mosquito's legs were digested to extract DNA. COII gene was amplified, sequenced and analysed; and the phylogenetic tree of members of An. minimus group was reconstructed by maximum likelihood method (DNAML).


CONCLUSIONS
The data confirmed the presence of two cryptic species, A and C, within An. minimus complex in the research localities; and two species differed by 2.3% in the COII sequences owing to 16 nucleotide substitutions. There was less variation between the two species than other members of the Minimus Group.
Zhou
Shui-sen
SS
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
Tang
Lin-hua
LH
Zheng
Xiang
X
D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D013045:Species Specificity
D004272:DNA, Mitochondrial; C117108:cytochrome C oxidase subunit II; D003576:Electron Transport Complex IV