Effect of factor VIII on tissue factor-initiated spatial clot growth.
Using time-lapse videomicroscopy, we studied the role of coagulation factor VIII (fVIII) in tissue factor-initiated spatial clot growth on fibroblast monolayers in a thin layer of non-stirred recalcified plasma from healthy donors or patients with severe Haemophilia A. Analysis of temporal evolution of light-scattering profiles from a growing clot revealed existence of two phases in the clot growth-initiation phase in a narrow (0.2 mm) zone adjacent to activator surface and elongation phase in plasma volume. While the initiation phase did not differ in normal and haemophilic plasmas, the rate of clot growth in the elongation phase in haemophilic plasma constituted only 30% of that in normal plasma. Supplementation of haemophilic plasma with 0.05 U/ml fVIII restored the normal clot growth rate (44.9 +/- 2.5 microm/min) at high but not at low fibroblast density. Our results indicate that the functioning of the intrinsic tenase complex is critical for normal spatial clot growth.
Ovanesov
Mikhail V
MV
Laboratory of Physical Biochemistry of Blood, Russian Academy of Medical Sciences, Moscow, Russia.
Lopatina
Elena G
EG
Saenko
Evgueni L
EL
Ananyeva
Natalya M
NM
Ul'yanova
Ljudmila I
LI
Plyushch
Olga P
OP
Butilin
Andrey A
AA
Ataullakhanov
Fazly I
FI
D001777:Blood Coagulation / Q000502:physiology*; D002452:Cell Count; D002478:Cells, Cultured / Q000166:cytology; D003546:Cysteine Endopeptidases / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D005169:Factor VIII / Q000008:administration & dosage / Q000494:pharmacology / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology; D006467:Hemophilia A / Q000097:blood*; D006801:Humans; D046911:Macromolecular Substances; D009363:Neoplasm Proteins / Q000502:physiology*; D009391:Nephelometry and Turbidimetry; D013997:Time Factors
D046911:Macromolecular Substances; D009363:Neoplasm Proteins; D005169:Factor VIII; D003546:Cysteine Endopeptidases; C030910:cancer procoagulant