Complexation of uranium (VI) by three eco-types of Acidithiobacillus ferrooxidans studied using time-resolved laser-induced fluorescence spectroscopy and infrared spectroscopy.
Time-resolved laser-induced fluorescence spectroscopy (TRLFS) was used to study the properties of uranium complexes (emission spectra and fluorescence lifetimes) formed by the cells of the three recently described eco-types of Acidithiobacillus ferrooxidans. The results demonstrated that these complexes have different lifetimes which increase in the same order as the capability of the strains to accumulate uranium. The complexes built by the cells of the eco-type II were the strongest, whereas, those of the eco-types I and III were significantly weaker. The emission spectra of all A. ferrooxidans complexes were almost identical to those of the uranyl organic phosphate compounds. The latter finding was confirmed by infrared spectroscopic analysis.
Merroun
Mohamed Larbi
ML
Institute of Radiochemistry, Forschungszentrum Rossendorf, D-01314 Dresden, Germany. m.merroun@fz-rossendorf.de
Geipel
Gerhard
G
Nicolai
Roswita
R
Heise
Karl-Heinz
KH
Selenska-Pobell
Sonja
S
D018533:Biomass; D004463:Ecology; D004785:Environmental Pollutants / Q000378:metabolism / Q000494:pharmacology; D020563:Gammaproteobacteria / Q000737:chemistry / Q000145:classification* / Q000187:drug effects / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007834:Lasers; D008826:Microbial Sensitivity Tests; D008906:Mining; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D014501:Uranium / Q000378:metabolism* / Q000494:pharmacology
D004785:Environmental Pollutants; D014501:Uranium