title: Eosinophil inflammation of nasal polyp tissue: relationships with matrix metalloproteinases, tissue inhibitor of metalloproteinase-1, and transforming growth factor-beta1.
abstract: Eosinophil and mast cell infiltrations are consistent findings in nasal polyp tissue. Previous studies have shown that matrix metalloproteinases (MMPs) may be involved in eosinophil infiltration in airway mucosa of asthmatic patients, and that transforming growth factor-beta1 (TGF-beta1) induces extracellular matrix deposition in nasal polyp tissue. The aim of this study was to evaluate the role of MMPs and tissue-inhibitor of metalloproteinase-1 (TIMP-1) in association with TGF-beta1, eosinophils and mast cell activation in nasal polyp tissue. Nasal polyp tissues from 20 patients who underwent polypectomies were collected and prepared into tissue homogenate. Eosinophil cationic protein (ECP) and tryptase levels were measured by CAP system (Pharmacia, Sweden). MMP-2, MMP-9, TIMP-1 and TGF-beta1 levels were measured by enzyme-liked immunosorbent assay. MMP-2 was the predominant form of MMPs, followed by MMP-9 and TIMP-1. There were significant correlations between ECP, and MMP-9, MMP-2, TGF-beta1 and tryptase, but not with TIMP-1. Significant correlations were noted between tryptase, and MMP-2, MMP-9, and TGF-beta1, but not with TIMP-1. Close correlations were noted between TGF-beta1, and MMP-9 and MMP-2, but not with TIMP-1. MMP-2, MMP-9, and TGF-beta1 may contribute to eosinophil and mast cell migrations into nasal polyp tissue.
authors.affiliation: Department of Allergy and Clinical Immunology, Ajou University School of Medicine, Suwon, Korea. hspark@madang.ajou.ac.kr
authors.forename: Young-Mok
authors.initials: YM
authors.lastname: Lee
authors.forename: Sun-Sin
authors.initials: SS
authors.lastname: Kim
authors.forename: Hyun-Ah
authors.initials: HA
authors.lastname: Kim
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Suh
authors.forename: Soo-Keol
authors.initials: SK
authors.lastname: Lee
authors.forename: Dong-Ho
authors.initials: DH
authors.lastname: Nahm
authors.forename: Hae-Sim
authors.initials: HS
authors.lastname: Park
mesh_terms: D000328:Adult; D001249:Asthma / Q000150:complications; D001798:Blood Proteins / Q000032:analysis; D002634:Chemotaxis, Leukocyte; D047091:Eosinophil Granule Proteins; D004802:Eosinophilia / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D004804:Eosinophils / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000502:physiology; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000502:physiology*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000502:physiology*; D008875:Middle Aged; D009298:Nasal Polyps / Q000737:chemistry* / Q000209:etiology / Q000473:pathology; D012220:Rhinitis / Q000378:metabolism / Q000473:pathology; D012260:Ribonucleases; D012697:Serine Endopeptidases / Q000032:analysis; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000502:physiology*; D053773:Transforming Growth Factor beta1; D053802:Tryptases
chemical_list: D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; C508960:TGFB1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D012260:Ribonucleases; D012697:Serine Endopeptidases; D053802:Tryptases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9