title: Genetic analysis of Crimean-Congo hemorrhagic fever virus in Russia.
abstract: Genetic analysis of wild-type Crimean-Congo hemorrhagic fever (CCHF) virus strains recovered in the European part of Russia was performed. Reverse transcriptase PCR followed by direct sequencing was used to recover partial sequences of the CCHF virus medium (M) genome segment (M segment) from four pools of Hyalomma marginatum ticks and six human patients. Phylogenetic analysis of the M-segment sequences from Russian strains revealed a close relatedness of the strains (nucleotide sequence diversity, <or=5.0%). The strains differed significantly from CCHF viruses from other regions of the world (nucleotide sequence diversity, 10.3 to 20.4%), suggesting that CCHF virus strains recovered in the European part of Russia form a distinct group.
authors.affiliation: State Research Center of Virology and Biotechnology "Vector," Koltsovo, Novosibirsk Region, 630559, Russia.
authors.forename: Lyudmila
authors.initials: L
authors.lastname: Yashina
authors.forename: Oleg
authors.initials: O
authors.lastname: Vyshemirskii
authors.forename: Sergei
authors.initials: S
authors.lastname: Seregin
authors.forename: Irina
authors.initials: I
authors.lastname: Petrova
authors.forename: Evgeny
authors.initials: E
authors.lastname: Samokhvalov
authors.forename: Dmitry
authors.initials: D
authors.lastname: Lvov
authors.forename: Valery
authors.initials: V
authors.lastname: Gutorov
authors.forename: Irina
authors.initials: I
authors.lastname: Kuzina
authors.forename: Georgy
authors.initials: G
authors.lastname: Tyunnikov
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Tang
authors.forename: Sergei
authors.initials: S
authors.lastname: Netesov
authors.forename: Vladimir
authors.initials: V
authors.lastname: Petrov
mesh_terms: D016679:Genome, Viral; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000145:classification / Q000235:genetics*; D006479:Hemorrhagic Fever, Crimean / Q000635:transmission / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012426:Russia
chemical_list: D012367:RNA, Viral