Expression of syntaxin 1C, an alternative splice variant of HPC-1/syntaxin 1A, is enhanced by phorbol-ester stimulation in astroglioma: participation of the PKC signaling pathway.
Syntaxin 1C is an alternative splice variant of HPC-1/syntaxin 1A; the latter participates in neurotransmitter release and is assigned to the gene domain responsible for Williams' syndrome (WS). It is expressed in the soluble fraction extracted from human astroglioma cell lines T98G and U87MG. Quantitative immunoblot and indirect immunofluorescence analyses revealed that the expression of syntaxin 1C was upregulated by phorbol 12-myristate 13-acetate (PMA), but not by forskolin. A protein kinase C (PKC) inhibitor suppressed this enhancement. These results suggest that syntaxin 1C expression is regulated via the PKC signal pathway. This is the first report of a signal transduction system that directly affects the expression of syntaxin protein.
Nakayama
Takahiro
T
Department of Physiology, Kyorin University School of Medicine, Tokyo 181-8611, Japan.nakayama@kyorin-u.ac.jp
Mikoshiba
Katsuhiko
K
Yamamori
Tetsuo
T
Akagawa
Kimio
K
D017398:Alternative Splicing; D000954:Antigens, Surface / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D001254:Astrocytoma; D001483:Base Sequence; D002460:Cell Line; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction; D050827:Syntaxin 1; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D004791:Enzyme Inhibitors; D009419:Nerve Tissue Proteins; C494212:STX1A protein, human; D050827:Syntaxin 1; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate