[Production of HBV surface antigen-specific target cell].
OBJECTIVE
To analyze the hepatitis B virus surface antigen (HBsAg)-specific cytotoxic T-cell (CTL) response during hepatitis development and investigate the effect of some factors on CTL response.


METHODS
The recombinant retrovirus plasmid PLXSN-S was tranferred into PA317 by electroporation and the pseudoviruses produced from PA317 were used to infect EL4. Clones which highly expressed HBsAg were selected with limited dilution.


RESULTS
A clone expressing-HBsAg the highest (A = 0.85 in supernatant at 48th hour) was obtained which steadly expressed HBsAg in vitro after at least 100 passages. It worked very well when used as target cells in CTL response after C57 mice immunized with PLXSN-S and recombinant adenovirus vector (rAdv-B7-2-S).


CONCLUSIONS
We constructed HBsAg-specific target cell line which steadly expressed HBsAg. It may play an important role in HBsAg CTL response and immunopathogenetic mechanisms of hepatitis B.
Zhou
Z
Z
Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010.
Zhang
D
D
Ren
H
H
D000818:Animals; D003001:Cloning, Molecular; D018274:Electroporation; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism*
D006514:Hepatitis B Surface Antigens; D011994:Recombinant Proteins