Participation of fibroblasts in MMP-2 binding and activation on the surface of oral squamous cell carcinoma cells.
The effect of fibroblasts on the activation of matrix metalloproteinase-2 (MMP-2) in oral squamous cell carcinoma (SCC) cells was examined. The plasma membrane of SCC cells failed to bind and activate latent MMP-2. However, treatment of SCC cells with fibroblast-conditioned medium (fibroblast-CM) led to the enhancement of the binding and activation of latent MMP-2 on the cell surface. Moreover, fibroblasts induced the expression of membrane type 1 MMP (MT1-MMP) in SCC cells. MMP-2 activated on the cell surface bound to the surface of SCC cells via alphav integrins. These findings suggest that fibroblasts might facilitate the invasion of SCC cells by increasing the proteolytic activity on the surfaces of SCC cells.
Hayashido
Yasutaka
Y
First Department of Oral and Maxillofacial Surgery, Hiroshima University School of Dentistry, Hiroshima 734-8553, Japan. hayashiy@hiroshima-u.ac.jp
Urabe
Kazuhiko
K
Yoshioka
Yukio
Y
Kitano
Hisataka
H
Okamoto
Tetsuji
T
Matsuya
Tokuzo
T
D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor / Q000201:enzymology; D002478:Cells, Cultured / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004789:Enzyme Activation; D004790:Enzyme Induction; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D053504:Matrix Metalloproteinases, Membrane-Associated; D008565:Membrane Proteins / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000096:biosynthesis* / Q000235:genetics; D009062:Mouth Neoplasms / Q000201:enzymology* / Q000473:pathology; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
D017077:Culture Media, Conditioned; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2