Microfluidic devices for DNA sequencing: sample preparation and electrophoretic analysis.
Modern DNA sequencing 'factories' have revolutionized biology by completing the human genome sequence, but in the race to completion we are left with inefficient, cumbersome, and costly macroscale processes and supporting facilities. During the same period, microfabricated DNA sequencing, sample processing and analysis devices have advanced rapidly toward the goal of a 'sequencing lab-on-a-chip'. Integrated microfluidic processing dramatically reduces analysis time and reagent consumption, and eliminates costly and unreliable macroscale robotics and laboratory apparatus. A microfabricated device for high-throughput DNA sequencing that couples clone isolation, template amplification, Sanger extension, purification, and electrophoretic analysis in a single microfluidic circuit is now attainable.
Paegel
Brian M
BM
Department of Chemistry, University of California, Berkeley, CA 94720, USA.
Blazej
Robert G
RG
Mathies
Richard A
RA
D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D008969:Molecular Sequence Data; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D016133:Polymerase Chain Reaction; D012212:Rheology / Q000295:instrumentation* / Q000379:methods; D012371:Robotics / Q000295:instrumentation / Q000379:methods; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000379:methods*; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods; D018511:Systems Integration
D004247:DNA