title: Effect of reuterin produced by Lactobacillus reuteri on the surface of sausages to inhibit the growth of Listeria monocytogenes and Salmonella spp.
abstract: Reuterin is a bacteriocin produced by some strains of Lactobacillus reuteri. The strain used in this study was isolated from raw milk from a dairy farm nearby Ankara. Beef sausage is a long years produced bratwurst style meat product in Turkey, as well as in some other countries in the Mediterranean region. Sausages are produced by raw meat; sometimes lactic starter cultures are added or spontaneous fermentation is employed. The production and storage conditions of the product promotes the growth of Listeria monocytogenes and Salmonella spp. Although nitrate is added as an antimicrobial substance against many pathogens, sometimes however nitrate application is not preventive enough on the surface because of the natural film around the sausages. Since most of the contaminations take place at post production steps, pathogenic growth is more effective on the surface of the sausages in refrigerated conditions. In this study, reuterin was applied to the surface of the sausages in order to prevent the growth of these two pathogens along with nitrate used as an additive in the product. Reuterin has inhibited the growth of L. monocytogenes considerably but not of Salmonella spp. on the surface of the sausages.
authors.affiliation: University of Ankara, Department of Food Engineering, TR-06110 Diskapi-Ankara, Turkey. sennaus@yahoo.com
authors.forename: Hakan
authors.initials: H
authors.lastname: Kuleaşan
authors.forename: M Lütfü
authors.initials: ML
authors.lastname: Cakmakçi
mesh_terms: D000447:Aldehydes; D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology*; D005506:Food Contamination / Q000517:prevention & control; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D005985:Glyceraldehyde / Q000031:analogs & derivatives; D007778:Lactobacillus / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D008461:Meat Products / Q000032:analysis / Q000382:microbiology*; D009566:Nitrates / Q000494:pharmacology; D011407:Propane; D012475:Salmonella / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000447:Aldehydes; D000900:Anti-Bacterial Agents; D009566:Nitrates; C047158:3-hydroxypropionaldehyde; D005985:Glyceraldehyde; D011407:Propane