title: Distinct roles of ephrin-B2 forward and EphB4 reverse signaling in endothelial cells.
abstract: OBJECTIVE
  The transmembrane ligand ephrin-B2 and its receptor tyrosine kinase EphB4 are specifically expressed on arterial and venous endothelial cells, respectively, and bidirectional signals mediated by both proteins play an important role in vascular development. However, how such bidirectional signals are required for cell-cell adhesion or repulsion remains unclear.
  
  
  RESULTS
  Using a cell line and sorted primary endothelial cells, we show that ephrin-B2 forward signaling through the EphB4 receptor inhibits cell adhesion, whereas EphB4 reverse signaling by the transmembrane ephrin-B2 ligand does not. Cell migration is also inhibited on immobilized ephrin-B2-Fc but not on EphB4-Fc protein.
  
  
  CONCLUSIONS
  Ephrin-B2 forward signaling and EphB4 reverse signaling differentially affect cell adhesion and migration between arterial and venous endothelial cells.
authors.affiliation: Department of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Hamada
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Oike
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Maekawa
authors.forename: Keishi
authors.initials: K
authors.lastname: Miyata
authors.forename: Taizo
authors.initials: T
authors.lastname: Shimomura
authors.forename: Toshio
authors.initials: T
authors.lastname: Suda
mesh_terms: D000199:Actins / Q000378:metabolism / Q000502:physiology; D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D001921:Brain / Q000098:blood supply; D002196:Capillaries / Q000166:cytology; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D003598:Cytoskeletal Proteins / Q000378:metabolism / Q000502:physiology; D003599:Cytoskeleton / Q000378:metabolism / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology*; D036388:Ephrin-B2 / Q000494:pharmacology / Q000502:physiology*; D007141:Immunoglobulin Fc Fragments / Q000494:pharmacology; D008024:Ligands; D051379:Mice; D036224:Receptor, EphB4 / Q000378:metabolism / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; D036388:Ephrin-B2; D007141:Immunoglobulin Fc Fragments; D008024:Ligands; D011993:Recombinant Fusion Proteins; D036224:Receptor, EphB4