Single-cell electroporation.
Electroporation is a widely used method for the introduction of polar and charged agents such as dyes, drugs, DNA, RNA, proteins, peptides, and amino acids into cells. Traditionally, electroporation is performed with large electrodes in a batch mode for treatment of a large number of cells in suspension. Recently, microelectrodes that can produce extremely localized electric fields, such as solid carbon fiber microelectrodes, electrolyte-filled capillaries and micropipettes as well as chip-based microfabricated electrode arrays, have proven useful to electroporate single cells and subcellular structures. Single-cell electroporation opens up a new window of opportunities in manipulating the genetic, metabolic, and synthetic contents of single targeted cells in tissue slices, cell cultures, in microfluidic channels or at specific loci on a chip-based device.
Olofsson
Jessica
J
Department of Physical Chemistry and Microtechnology Centre, Chalmers, SE-412 96, Göteborg, Sweden.
Nolkrantz
Kerstin
K
Ryttsén
Frida
F
Lambie
Bradley A
BA
Weber
Stephen G
SG
Orwar
Owe
O
D002463:Cell Membrane Permeability / Q000502:physiology; D002478:Cells, Cultured; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004574:Electromagnetic Fields; D018274:Electroporation / Q000295:instrumentation* / Q000379:methods* / Q000639:trends; D004867:Equipment Design; D008839:Microelectrodes; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods