[Experimental study on the apoptotic effect of arsenic trioxide on human osteosarcoma MG-63 cells].
OBJECTIVE
To study the apoptotic effect of arsenic trioxide(As2 O3) on human ostrosarcoma MG-63 cells.


METHODS
The MTT reduction test, transmission electron microscopy and agarose gel electrophoresis were applied to observe the apoptotic changes of human osteosarcoma line MG-63 induced by As2 O3.


RESULTS
Typical apoptotic cellular changes were found after As2 O3 treatment. Transmission electron miroscopy showed that nucleolus disappeared and chromatin condensed under the nucleonic membrane. Agarose gel electrophorsis showed a "ladder" strand of DNA, a special phenomenon of cellular apoptosis. The above-mentioned changes were not found in the control group.


CONCLUSIONS
As2 O3 can induce apoptosis of human osteosarcoma MG-63 cells. Further studies are to be carried out on its mechanism.
Xiao
Tao
T
Department of Othopaedics, Xiangya Hospital, Central South University, Changsha 410008, China.
Li
Kang-hua
KH
Fang
Jian-zhen
JZ
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D001859:Bone Neoplasms / Q000473:pathology; D006801:Humans; D012516:Osteosarcoma / Q000473:pathology*; D010087:Oxides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D001152:Arsenicals; D010087:Oxides; D000077237:Arsenic Trioxide