title: Expression of the nidogen-binding site of the laminin gamma1 chain disturbs basement membrane formation and maintenance in F9 embryoid bodies.
abstract: Basement membranes contain two major molecular networks consisting of laminin and collagen IV. Previous antibody perturbation experiments suggest that the interaction between laminin and nidogen-1 is necessary for proper basement membrane formation and epithelial development, whereas results from gene ablation experiments in mice show that both basement membranes and general development are grossly normal in the absence of nidogen-1. To refine the perturbation approach, we produced F9-teratocarcinoma-cell-derived embryoid bodies in the presence of recombinantly expressed nidogen-binding sites localized within the gamma1III3-5 laminin fragment. We found basement membranes were disrupted in gamma1III3-5-expressing embryoid bodies. As a measurement of basement membrane function, we tested permeability and detected drastically increased diffusion rates in correlation with basement membrane disruption. Furthermore, TROMA-1 localization in embryoid bodies expressing the nidogen-binding site was altered, suggesting separation of epithelium-specific gene expression from the formation of the actual epithelium when occurring in the absence of an organized basement membrane.
authors.affiliation: Center for Biochemistry, Medical Faculty, University of Cologne, D-50931 Cologne, Germany.
authors.forename: Judith
authors.initials: J
authors.lastname: Tunggal
authors.forename: Maria
authors.initials: M
authors.lastname: Wartenberg
authors.forename: Mats
authors.initials: M
authors.lastname: Paulsson
authors.forename: Neil
authors.initials: N
authors.lastname: Smyth
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D001665:Binding Sites / Q000235:genetics; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D004058:Diffusion; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D015151:Immunoblotting; D007797:Laminin / Q000235:genetics / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D014407:Tumor Cells, Cultured
chemical_list: D007797:Laminin; D008562:Membrane Glycoproteins; C089141:laminin gamma 1; C029967:nidogen