Partition coefficients for benzene in human skin.
The contribution of benzene to body burden after skin absorption compared with that due to inhalation absorption is of potential interest in the setting and interpretation of benzene (inhalation) exposure standards. However, an understanding of the quantitative relationship between skin and inhalation absorption, under different exposure conditions, is required. Such knowledge may be gained through physiological based pharmacokinetic (PBPK) modeling. The intake of benzene to the body via inhalation has been studied extensively. Physiological parameters enabling the calculation of amounts of benzene entering the blood stream per unit time are readily available for use in a PBPK model. Unfortunately, some data (i.e., partition coefficients) that would enable biologically plausible calculation of amounts of benzene entering the blood stream via skin absorption in a PBPK model are not available. Hence, the aim of this research was to determine partition coefficients across the epidermal and dermal layers of human skin so that these could be used within a PBPK model to determine quantitatively the flow rate of benzene per unit time through intact skin into the blood stream. The partition coefficients found for blood substitute: viable epidermis and blood substitute: dermis were, respectively, 2.4 and 11.2. Partition coefficients for benzene : stratum corneum (4.2), whole skin : blood substitute (2.2), benzene : water (109/126), and benzene : blood substitute (55/59) also were determined for the purposes of validating the blood substitute: viable epidermis and blood substitute : dermis partition coefficients.
Sinclair
Georgia C
GC
Deakin University, P.O. Box 210, Glen Iris, Victoria 3146, Australia. georgia.sinclair@bigpond.com
Wester
Ronald C
RC
Maibach
Howard I
HI
D001554:Benzene / Q000493:pharmacokinetics*; D001822:Body Burden; D004817:Epidermis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D019570:Inhalation Exposure / Q000009:adverse effects / Q000032:analysis; D008452:Maximum Allowable Concentration; D008954:Models, Biological; D016273:Occupational Exposure / Q000009:adverse effects / Q000032:analysis; D012869:Skin Absorption; D014481:United States
D001554:Benzene