Serglycin proteoglycan expression and synthesis in embryonic stem cells.
The serglycin proteoglycan is expressed in most hematopoietic cells and is packaged into secretory vesicles for constitutive or regulated secretion. We have now shown serglycin mRNA expression in undifferentiated murine embryonic stem (ES) cells and in embryoid bodies, and synthesis and secretion in undifferentiated ES cells. Serglycin was localized to ES cell cytoplasm by immunostaining. Serglycin mRNA is expressed in tal-1((-/-)) ES cells and embryoid bodies; tal-1((-/-)) mice cannot produce hematopoietic cells. Thus, ES serglycin expression is probably not associated with hematopoiesis. Serglycin expression was increased by treatment of ES cells with retinoic acid (RA) and dibutyryl cAMP (dbcAMP). The serglycin core protein obtained from control ES culture medium after chondroitinase digestion appears as a doublet. Only the lower Mr band is present in serglycin secreted from RA-treated and the higher Mr band in RA+dbcAMP-treated cells, suggesting that core protein structure is affected by differentiation.
Schick
Barbara P
BP
Cardeza Foundation for Hematologic Research, Department of Medicine, Jefferson Medical College of Thomas Jefferson University, 1015 Walnut Street, Philadelphia, PA 19107, USA. barbara.schick@mail.tju.edu
Ho
Hon-Chung Keith
HC
Brodbeck
Kristin C
KC
Wrigley
Clinton W
CW
Klimas
Joanne
J
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation; D002460:Cell Line; D019765:Chondroitin ABC Lyase; D006410:Hematopoiesis; D051379:Mice; D011509:Proteoglycans / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013234:Stem Cells / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology; D033921:Vesicular Transport Proteins
D011509:Proteoglycans; D012333:RNA, Messenger; D033921:Vesicular Transport Proteins; C075430:serglycin; D014212:Tretinoin; D003994:Bucladesine; D019765:Chondroitin ABC Lyase