[Effect of Callicarpa herbs on antioxidation in vitro].
The concentration of half inhibition (IC50) of Callicarpa herbs, including Callicarpa kochiana(Ck), C. bodinieri (Cb) and its fruit (Cbf), C. japonica, C. macrophrlla, C. cathaarana, C. giraldi(Cg) on lipid peroxidation (LPO) of tissus homogenate incubated in 37 +/- 0.5 degrees C were tested. The result showed that all but Cg could inhibit lipid peroxidation. The IC50 of Cbf was minimum. IC50 of Cb, Cbf and Ck inhibiting red blood cell (RBC) LPO and hemolysis caused by hydroxyl free radical (.OH), inhibiting brain homogenate LPO induced by .OH and inhibiting or clean .OH formation by system of H2O2 and FeSO4 were also tested. It was found the IC50 were Cbf > Cb > Ck. These results suggested that the most strong inhibitor to automatic LPO reaction was Cbf, but to LPO caused by .OH was Ck.
Jiang
H
H
School of Pharmacy, Zhejing University, 310031.
Ji
Y
Y
Zhang
S
S
D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism; D032408:Callicarpa; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D017665:Hydroxyl Radical / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal
D000975:Antioxidants; D010936:Plant Extracts; D017665:Hydroxyl Radical