title: The role of an evolutionarily conserved cis-proline in the thioredoxin-like domain of human class Alpha glutathione transferase A1-1.
abstract: The thioredoxin-like fold has a betaalphabetaalphabetabetaalpha topology, and most proteins/domains with this fold have a topologically conserved cis -proline residue at the N-terminus of beta-strand 3. This residue plays an important role in the catalytic function and stability of thioredoxin-like proteins, but is reported not to contribute towards the stability of glutathione S-transferases (GSTs) [Allocati, Casalone, Masulli, Caccarelli, Carletti, Parker and Di Ilio (1999) FEBS Lett. 445, 347-350]. In order to further address the role of the cis -proline in the structure, function and stability of GSTs, cis -Pro-56 in human GST (hGST) A1-1 was replaced with a glycine, and the properties of the P56G mutant were compared with those of the wild-type protein. Not only was the catalytic function of the mutant dramatically reduced, so was its conformational stability, as indicated by equilibrium unfolding and unfolding kinetics experiments with urea as denaturant. These findings are discussed in the context of other thioredoxin-like proteins.
authors.affiliation: Protein Structure-Function Research Programme, School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg 2050, South Africa.
authors.forename: Chris
authors.initials: C
authors.lastname: Nathaniel
authors.forename: Louise A
authors.initials: LA
authors.lastname: Wallace
authors.forename: Jonathan
authors.initials: J
authors.lastname: Burke
authors.forename: Heini W
authors.initials: HW
authors.lastname: Dirr
mesh_terms: D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes; D007700:Kinetics; D009154:Mutation; D011392:Proline / Q000378:metabolism*; D011489:Protein Denaturation / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology; D013050:Spectrometry, Fluorescence; D014508:Urea / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D014508:Urea; D011392:Proline; D005982:Glutathione Transferase; C119995:glutathione S-transferase alpha; D005998:Glycine