title: Isolation and characterization of Staufen-containing ribonucleoprotein particles from rat brain.
abstract: Localized mRNAs are thought to be transported in defined particles to their final destination. These particles represent large protein complexes that may be involved in recognizing, transporting, and anchoring localized messages. Few components of these ribonucleoparticles, however, have been identified yet. We chose the strategy to biochemically enrich native RNA-protein complexes involved in RNA transport to identify the associated RNAs and proteins. Because Staufen proteins were implicated in intracellular RNA transport, we chose mammalian Staufen proteins as markers for the purification of RNA transport particles. Here, we present evidence that Staufen proteins exist in two different complexes: (i) distinct large, ribosome- and endoplasmic reticulum-containing granules preferentially found in the membrane pellets during differential centrifugation and (ii) smaller particles in the S100 from rat brain homogenates. On gel filtration of the S100, we identified soluble 670-kDa Staufen1-containing and 440-kDa Staufen2-containing particles. They do not cofractionate with ribosomes and endoplasmic reticulum but rather coenrich with kinesin heavy chain. Furthermore, the fractions containing the Staufen1 particles show a 15-fold enrichment of mRNAs compared with control fractions. Most importantly, these fractions are highly enriched in BC1, and, to a lesser extent, in the alpha-subunit of the Ca(2+)/calmodulin-dependent kinase II, two dendritically localized RNAs. Finally, both RNAs colocalize with Staufen1-hemagglutinin in particles in dendrites of transfected hippocampal neurons. We therefore propose that these Staufen1-containing particles may represent RNA transport intermediates that are in transit to their final destination within neurons.
authors.affiliation: Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.
authors.forename: Massimo
authors.initials: M
authors.lastname: Mallardo
authors.forename: Anke
authors.initials: A
authors.lastname: Deitinghoff
authors.forename: Juliane
authors.initials: J
authors.lastname: Müller
authors.forename: Bernhard
authors.initials: B
authors.lastname: Goetze
authors.forename: Paolo
authors.initials: P
authors.lastname: Macchi
authors.forename: Christopher
authors.initials: C
authors.lastname: Peters
authors.forename: Michael A
authors.initials: MA
authors.lastname: Kiebler
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D017403:In Situ Hybridization; D016601:RNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016601:RNA-Binding Proteins; C492186:Stau1 protein, rat; C492188:Stau2 protein, rat; C117884:staufen protein, mammalian