CRALBP transcriptional regulation in ciliary epithelial, retinal Müller and retinal pigment epithelial cells.
Cellular retinaldehyde binding protein (CRALBP) functions in the visual cycle and mutations in the RLBP1 gene can lead to blindness. RLBP1 promoter analyses have been pursued in vitro as an approach to deciphering the mechanism controlling cell-specific expression of CRALBP. Reporter activity of wildtype and mutant RLBP1 promoter constructs suggest that CRALBP transcriptional regulation may be similar in the ciliary epithelium (CE) and retinal pigment epithelium (RPE) but different in Müller cells. Results in RPE cells refine the location of an RLBP1 enhancer element to within -1826 to -1749 bp and a repressor element to within -702 to -635 bp.
Kennedy
Breandán N
BN
Li
Chibo
C
Ortego
Javier
J
Coca-Prados
Miguel
M
Sarthy
Vijay P
VP
Crabb
John W
JW
D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002478:Cells, Cultured; D002924:Ciliary Body / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D010857:Pigment Epithelium of Eye / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012160:Retina / Q000378:metabolism*; D014158:Transcription, Genetic
C020932:11-cis-retinal-binding protein; D002352:Carrier Proteins