Aggressiveness and quantification of epithelial proliferation of middle ear cholesteatoma by MIB1.
OBJECTIVE
To assess an easy method that predicts cholesteatoma aggressiveness.


METHODS
An experimental prospective study.


METHODS
Monoclonal antibody MIB1 was used to determine epithelium proliferation in 91 cholesteatomatous ears. Clinical and surgical parameters were compared with proliferation activity to determine pathological and clinical correlation.


RESULTS
Statistical correlations were established between hyperproliferation of the cholesteatoma and severe bone erosion (leading to major cholesteatoma complications) and between hyperproliferation and middle ear inflammation (associated with more surgical difficulties and a higher risk of recurrence). A high proliferation index was also found in children's cholesteatoma, which is known to have more aggressive behavior.


CONCLUSIONS
Immunohistochemical use of the MIB1 antibody is a simple technique that can help to determine the aggressiveness of a cholesteatoma.
Mallet
Y
Y
Departments of Otorhinolaryngology, Centre Hospitalier Universitaire Lille, Lille, France. y-mallet@o-lambret
Nouwen
J
J
Lecomte-Houcke
M
M
Desaulty
A
A
D000293:Adolescent; D000328:Adult; D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D002455:Cell Division; D002648:Child; D018424:Cholesteatoma, Middle Ear / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D004429:Ear Ossicles / Q000473:pathology; D004432:Ear, Middle / Q000473:pathology*; D004848:Epithelium / Q000737:chemistry / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis / Q000276:immunology; D008875:Middle Aged; D011446:Prospective Studies
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D019394:Ki-67 Antigen; C440408:MIB-1 antibody