Soybean beta-conglycinin peptone suppresses food intake and gastric emptying by increasing plasma cholecystokinin levels in rats.
Cholecystokinin (CCK) is an important physiologic mediator that regulates satiety and gastric emptying. We demonstrated previously that soybean peptone acts directly on rat small intestinal mucosal cells to stimulate CCK release. In the present study, we examined the effects of beta-conglycinin, a major component of soy protein, and its peptone on food intake and gastric emptying after an intraduodenal infusion of beta-conglycinin peptone in relation to CCK release and interaction with the mucosal cell membrane. Intraduodenal infusion of beta-conglycinin peptone inhibited food intake in a dose-dependent manner, but that of whole soy peptone or camostat did not. The suppression of food intake by beta-conglycinin peptone was abolished by an intravenous injection of devazepide, a selective peripheral CCK receptor antagonist. The beta-conglycinin peptone infusion strongly suppressed gastric emptying with marked increases in portal CCK levels. We also observed that the beta-conglycinin peptone dose dependently and more potently stimulated CCK release from isolated dispersed mucosal cells of the rat jejunum than did beta-conglycinin itself. This stimulation corresponded to the binding activity of the peptide or protein to solubilized components of the rat jejunum membrane as evaluated by surface plasmon biosensor. These results indicate that beta-conglycinin peptone suppresses food intake, and this effect may be due to beta-conglycinin peptone in the lumen stimulating endogenous CCK release with direct acceptance to the intestinal cells.
Nishi
Takashi
T
Northern Advancement Center for Science and Technology, Colabo-Hokkaido, Sapporo 001-0021, Japan.
Hara
Hiroshi
H
Tomita
Fusao
F
D000818:Animals; D052179:Antigens, Plant; D002766:Cholecystokinin / Q000097:blood*; D020109:Devazepide / Q000494:pharmacology; D004435:Eating / Q000187:drug effects*; D005746:Gastric Emptying / Q000187:drug effects*; D005916:Globulins / Q000494:pharmacology* / Q000502:physiology; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D043886:Receptor, Cholecystokinin A; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors; D055314:Seed Storage Proteins; D030262:Soybean Proteins / Q000494:pharmacology* / Q000502:physiology
D052179:Antigens, Plant; D005916:Globulins; D043886:Receptor, Cholecystokinin A; D011949:Receptors, Cholecystokinin; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; D002766:Cholecystokinin; D020109:Devazepide