The preliminary study of glucocorticoid receptor gene in Chinese patients with glucocorticoid-induced glaucoma.
BACKGROUND
To study the glucocorticoid receptor (GR) and the associated gene regulation in the pathogenesis of glucocorticoid-induced glaucoma (GIG) in Chinese patients.


METHODS
The trabecular cells of normal individuals and patients with GIG were cultured in vitro. By using polymerase chain reaction (PCR), gene fragments on GR DNA binding sites of trabecular cells were amplified. The product was detected by gel electrophoresis.


RESULTS
The trabecular cells were cultured successfully in normal individuals and patients with GIG in vitro. A single PCR product was obtained in both two groups with the same size of 545 base pairs.


CONCLUSIONS
There is not any difference in gene on the GR DNA binding sites between normal individuals and patients with GIG. The results suggest the difference in mRNA or other functional genes.
Zhuo
Y
Y
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Ge
J
J
Guo
Y
Y
Lin
M
M
D001665:Binding Sites; D002478:Cells, Cultured; D002681:China; D004247:DNA / Q000235:genetics; D003907:Dexamethasone; D004587:Electrophoresis, Agar Gel; D005901:Glaucoma / Q000139:chemically induced / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D011965:Receptors, Glucocorticoid / Q000235:genetics*; D014129:Trabecular Meshwork / Q000166:cytology / Q000473:pathology*
D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone; D004247:DNA