[The comparison of contents of uracil, xanthine and hypoxanthine in five species of leech].
Three constituents(uracil, xanthine, hypoxanthine) were isolated from five species of leech and determined by reversed phase HPLC. The column employed was Shim-pack CLC-ODS C18(150 mm x 6 mm). The mobile phase was 0.05 mol/l ammonium phophate dibasic solution(pH = 8.4). The flow rate was 0.8 ml/min and detection was effected at 254 nm. This method is accurate, rapid and reproducible. Analytical data for five species and Whitmania pigra samples from different places were given.
Liu
L
L
Department of Chinese Pharmacogonosy, China Pharmaceutical University, Nanjing 210038.
Jin
R
R
Xu
G
G
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019271:Hypoxanthine / Q000032:analysis*; D007865:Leeches / Q000737:chemistry* / Q000145:classification; D008419:Materia Medica / Q000737:chemistry*; D014498:Uracil / Q000032:analysis*; D019820:Xanthine / Q000032:analysis*
D008419:Materia Medica; D019820:Xanthine; D019271:Hypoxanthine; D014498:Uracil