title: [Influence of selenium-rich rice on transformation of umbilical blood B lymphocytes by Epstein-Barr virus and Epstein-Barr virus early antigen expression].
abstract: OBJECTIVE
  Selenium (Se), an antioxidant, is an essential trace element to human body. It can be used as an anti-aging agent and a tumor cell proliferation inhibitor. To further investigate the effect of selenium in cancer prevention, the authors observed the influence of Se-rich rice extract on the transformation of umbilical blood B lymphocytes stimulated by Epstein-Barr virus (EBV) and expression of EBV early antigen(EBV-EA) in Raji cells.
  
  
  METHODS
  (1) Se-rich rice and general rice extract (dilution of 1:4 or 1:8) were added to mixture of EBV, and then umbilical blood mononuclear cells were added. Lymphoblasts transformation test was then performed. The inhibition rate of B lymphocytes transformation was calculated. (2) Raji cells stimulated by butyrate and croton oil were incubated with Se-rich rice extract. The EBV-EA positive expression rate and the inhibition rate were counted using indirect immunological flurescence method.
  
  
  RESULTS
  The transformation of umbilical blood B lymphocytes stimulated by EBV was significantly inhibited by Se-rich rice extract at a concentration of 0.11 g/ml (1:8 diluted). The inhibition rate was 83.4% (P < 0.01), which was significantly higher than that of the control rice (63.1%) (P < 0.05). Se-rich rice extract showed significant inhibition on EBV-EA in Raji cells. As the extract concentration was at 0.016 microgram/ml, 0.078 g/ml, and 0.388 microgram/ml, the inhibition rates of EA were 2.85%, 12.88%, and 20.75%, respectively.
  
  
  CONCLUSIONS
  The transformation of umbilical blood B lymphocytes stimulated by EB virus and expression of EBV-EA in Raji cells may be significantly inhibited by Se-rich rice extract, suggesting that Se-rich rice can be used for preventing nasopharyngeal carcinoma.
authors.affiliation: Cancer Institute, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, P. R. China.
authors.forename: Shao-Wen
authors.initials: SW
authors.lastname: Jian
authors.forename: Cheng-En
authors.initials: CE
authors.lastname: Mei
authors.forename: Yong-Neng
authors.initials: YN
authors.lastname: Liang
authors.forename: Duan
authors.initials: D
authors.lastname: Li
authors.forename: Qiao-Lun
authors.initials: QL
authors.lastname: Chen
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Luo
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Li
authors.forename: Ti-Yu
authors.initials: TY
authors.lastname: Cai
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D000956:Antigens, Viral / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000821:virology; D045744:Cell Line, Tumor; D005312:Fetal Blood / Q000276:immunology; D004854:Herpesvirus 4, Human; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D016393:Lymphoma, B-Cell / Q000378:metabolism / Q000473:pathology; D012275:Oryza / Q000737:chemistry; D012643:Selenium / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D016588:Anticarcinogenic Agents; D000956:Antigens, Viral; C033305:Epstein-Barr virus early antigen; D012643:Selenium