title: Evaluation of five stains in diagnosing human intestinal coccidiosis.
abstract: Three hundreds cases over the year 1998 complaining of diarrhoea were examined. The stools were examined by the traditional diagnostic methods and confirmed to be free from intestinal parasites. Cryptosporidium parvum, Isospora belli and Cyclospora cayetanensis were detected by using different types of faecal stains namely modified Ziehl-Neelsen, Kinyoun acid-fast, Auramine-rhodamine, Gomori's trichrome and Giemsa. The number of positive cases were 87 C. parvum (29%), 5 cases L. belli (1.7%) and 12 cases C. cayetanensis (4%). This study showed that the sensitivity of modified Ziehl-Neelsen and Kinyoun Acid-fast were very high (100%) in comparison with the other stains.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Mansoura University, Egypt.
authors.forename: H H
authors.initials: HH
authors.lastname: El Naggar
authors.forename: A E
authors.initials: AE
authors.lastname: Handousa
authors.forename: E M
authors.initials: EM
authors.lastname: El Hamshary
authors.forename: A M
authors.initials: AM
authors.lastname: El Shazly
mesh_terms: D000818:Animals; D003048:Coccidiosis / Q000175:diagnosis* / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000302:isolation & purification; D021744:Cyclospora / Q000302:isolation & purification; D021741:Eimeriida / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology; D006801:Humans; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis* / Q000469:parasitology; D007549:Isospora / Q000302:isolation & purification; D033661:Oocysts / Q000302:isolation & purification; D010270:Parasite Egg Count; D010274:Parasitology / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*