Detection and characterization of leishmania antigens from an American cutaneous leishmaniasis vaccine for diagnosis of visceral leishmaniasis.
Antigens were isolated from vaccines against American Cutaneous Leishmaniasis (ACL) and their reactivity tested against nine different groups of human sera and two groups of dog sera. These antigens react specifically with human and dog visceral leishmaniasis sera when compared to sera from non-infected individuals. Sera from humans from endemic areas of ACL before, or one year after, vaccination, and ACL patients treated and cured by immunotherapy with polyvalent vaccine, did not display significant differences of reactivity to these antigens. In contrast, they displayed a significantly higher reactivity to the antigens when compared to sera from healthy humans from non-endemic areas. No sera reactivity was observed with patients carrying Chagas' disease or tuberculosis. These antigens are polysaccharides aggregates and present molecular masses ranging from 90 to over 200 KDa. These data suggest the use of these antigens for sero-diagnosis of human and canine visceral leishmaniasis.
Ferreira
Wanderley Almeida
WA
Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Minas Gerais, Brazil
Mayrink
Wilson
W
dos Mares-Guia
Marcos Luiz
ML
Tavares
Carlos Alberto Pereira
CA
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D004285:Dogs; D004587:Electrophoresis, Agar Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D007891:Leishmania / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000097:blood / Q000175:diagnosis* / Q000517:prevention & control*; D016052:Protozoan Vaccines / Q000032:analysis* / Q000494:pharmacology; D012680:Sensitivity and Specificity
D000953:Antigens, Protozoan; D016052:Protozoan Vaccines