[Detection of anti-Spirometra erinaceieuropaei antibody using ELISA].
OBJECTIVE
To study specific diagnosis of Spirometra erinaceieuropaei.


METHODS
An enzyme-linked immunosorbent assay (ELISA) was studied using highly pure gene engineering antigen expressed by the recombination of the cloned cysteine proteinase gene of Spirametra erinaceieuropaei with expression vector pMAL-c2. Six sera from patient infected with Spirometra erinaceieuropaei were detected using this method.


CONCLUSIONS
The results showed that the gene engineering antigen reacted strongly with the sera from Spirometra erinaceieuropaei-infected patients, but did not with the sera from Cysticercus cellulosae-infected patients.
Ding
Y X
YX
Department of Epidemiology, Hebei Medical University, Shijiazhuang 050017.
Guo
L L
LL
Liu
D W
DW
Zhang
P H
PH
Liu
S X
SX
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology; D002590:Cestode Infections / Q000175:diagnosis* / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D012680:Sensitivity and Specificity; D013146:Spirometra / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth