title: [Regulation of Gal beta 1,3GalNAc alpha 2,3-sialyltransferase (ST3GalI) by hepatitis B virus MHBst/HBx transactivator].
abstract: Hepatitis B virus MHBst and HBx fragments were amplified to construct eukaryotic expression vector pCDNA3.1-MHBst and pCDNA3.1-HBx. ST3GalI promoter region was obtained by the method of PCR and GFP report plasmid pEGFP-N1-Psial was constructed. pCDNA3.1-MHBst or pCDNA3.1-HBx with pEGFP-N1-Psial were transiently co-transfected into QGY-7701 cells using calcium phosphate-DNA co-precipitation, respectively. The expressions of Psial-directed GFP were analyzed by FAC-Scalibur. It was found that MHBst/HBx could upregulate ST3GalI promoter activity by 35.2% and 43.8%, respectively. We report the regulation of ST3GalI by MHBst and HBx transactivators. It would be helpful to further investigate the relation between hepatitis B virus infection and sialyltransferase expression.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Ding
authors.forename: Jun-Qi
authors.initials: JQ
authors.lastname: Wang
authors.forename: Cheng
authors.initials: C
authors.lastname: Jin
mesh_terms: D015971:Gene Expression Regulation, Enzymologic; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000502:physiology*; D006515:Hepatitis B virus; D011401:Promoter Regions, Genetic; D012799:Sialyltransferases / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D006514:Hepatitis B Surface Antigens; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D012799:Sialyltransferases; C029156:N-acetyllactosaminide alpha-2,3-sialyltransferase