Syndecan-1 and -4 synthesized simultaneously by mouse mammary gland epithelial cells bear heparan sulfate chains that are apparently structurally indistinguishable.
Many of the biological functions attributed to cell surface heparan sulfate (HS) proteoglycans, including the Syndecan family, are elicited through the interaction of their HS chains with soluble extracellular molecules. Tightly controlled, cell-specific sulfation and epimerization of HS precursors endows these chains with highly sulfated, iduronate-rich regions, which are major determinants of cytokine and matrix-protein binding and which are interspersed by N-acetylated, poorly sulfated regions. Until this study, there have been no comprehensive structural comparisons made on HS chains decorating simultaneously expressed, but different, syndecan core proteins. In this paper we demonstrate that the HS chains on affinity-purified syndecan-1 and -4 from murine mammary gland cells are essentially identical by a number of parameters. Size determination, disaccharide analyses, enzymatic and chemical scission methods, and affinity co-electrophoresis all failed to reveal any significant differences in fine structure, domain organization, or ligand-binding properties of these HS species. These findings lead us to suggest that the imposition of the fine structure onto HS occurs independently of the core protein to which it is attached and that these core proteins, in addition to the HS chains, may play a pivotal role in the various biological functions ascribed to these macromolecules.
Zako
Masahiro
M
Division of Newborn Medicine, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02215, USA. zako@aichi-med.ac.jp
Dong
Jianying
J
Goldberger
Olga
O
Bernfield
Merton
M
Gallagher
John T
JT
Deakin
Jon A
JA
D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000096:biosynthesis / Q000302:isolation & purification; D004187:Disaccharides / Q000737:chemistry / Q000302:isolation & purification; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D019763:Heparin Lyase / Q000378:metabolism; D006497:Heparan Sulfate / Q000737:chemistry* / Q000302:isolation & purification; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry*; D051379:Mice; D011509:Proteoglycans / Q000096:biosynthesis* / Q000737:chemistry*; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes; D053668:Syndecan-1; D053671:Syndecan-4; D053667:Syndecans
D003094:Collagen; D004187:Disaccharides; D019763:Heparin Lyase; D006497:Heparan Sulfate; D008562:Membrane Glycoproteins; D011509:Proteoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes; D053668:Syndecan-1; D053671:Syndecan-4; D053667:Syndecans; C508242:Sdc1 protein, mouse; C508306:Sdc4 protein, mouse