Interleukin-1 facilitates airway epithelial migration in response to injury.
OBJECTIVE
To test the hypothesis that interleukin (IL)-1 plays a permissive role in respiratory epithelial cell migration and proliferation.


METHODS
Primary cultures of porcine respiratory epithelial cells or tracheal organ explants were cultured in the presence or absence of function-blocking antibodies to IL-1. Areas of epithelial cell outgrowth were determined in control and antibody-treated organ explants daily for 4 days. At intervals, cultured cells were collected for cell counting and viability determination. Time course and dose-response curves were constructed for control and antibody-treated groups.


RESULTS
Interleukin-1 secretion into culture supernatants increased sharply from days 3 to 7. Outgrowths from tracheal explants were reduced by greater than 60% by single antibody treatment, and by over 90% by treatment with antibodies to both IL-1alpha and -1beta by day 4 of culture. Function-blocking antibodies to IL-1 significantly reduced cell number by day 7 of culture.


CONCLUSIONS
Interleukin-1 is produced by respiratory epithelial cells in culture during log phase growth and plays a permissive role in cell migration and proliferation.
Hicks
Wesley
W
Department of Surgical Oncology, Head and Neck Surgery, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA. Wesley.Hicks@roswellpark.org
Hall
Leon A
LA
Tristram
Debra A
DA
Gardella
Joseph A
JA
Bright
Frank V
FV
Hard
Robert
R
Sigurdson
Lynn
L
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000494:pharmacology; D002455:Cell Division / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D007375:Interleukin-1 / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D009924:Organ Culture Techniques; D020545:Respiratory Mucosa / Q000166:cytology* / Q000378:metabolism; D013552:Swine; D014132:Trachea / Q000166:cytology
D000906:Antibodies; D007375:Interleukin-1