An enterotoxic activity of 18-20 kDa Cryptosporidium parvum secretory coproantigen (CCA 18-20 kDa).
An 18-20 kDa Cryptosporidum coproantigen (18-20 CCA), had been detected in the stool of infected humans and calves. A purified, electroeluted and concentrated 18-20 kDa antigen was tested in Ussing chamber, and electric parameters were tested before and after the addition of this antigen. A significant increase in the short circuit current (Isc) was detected. The enterotoxic effectof 18-20 kDa CCA, was time and dose dependent, heat labile, and Cl- dependent. The detected change in the short circuit current was not detected when the 18-20 kDa antigen wa incubated with its monospecific antibodies. These results indicate an enterotoxic activity of the 18-20 kDa antigen secreted from the released parasite and detected in stool of infected humans and calves. Additionally, they may help in developing an appropriate anti-secretory therapy for the intractable diarrhoea caused by cryptosporidiosis.
El-Shewy
K A
KA
Department of Parasitology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Hussien
A M
AM
El-Maghraby
D F
DF
D000818:Animals; D000953:Antigens, Protozoan / Q000633:toxicity*; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000469:parasitology / Q000662:veterinary; D016785:Cryptosporidium parvum / Q000276:immunology / Q000472:pathogenicity*; D004768:Enterotoxins / Q000633:toxicity*; D005243:Feces / Q000469:parasitology; D006801:Humans; D007082:Ileum / Q000469:parasitology / Q000473:pathology; D007413:Intestinal Mucosa / Q000469:parasitology / Q000473:pathology; D011817:Rabbits
D000953:Antigens, Protozoan; D004768:Enterotoxins