Tracking dendritic cells: use of an in situ method to label all blood leukocytes.
Here we describe an in situ procedure with a labeling index (percent of labeled blood leukocytes) >98%, which is high enough to permit the direct tracking of dendritic cell (DC) precursors from blood into lymphoid tissues, while circumventing the pitfalls associated with in vitro labeling. DC and lymphocytes have similar blood to afferent lymph migratory capabilities. This method has additional applications in tracking other rare cell populations in both normal and pathological states.
Ristevski
Bill
B
Department of Laboratory Medicine and Pathobiology, University of Toronto, 1 Kings' College Circle, Toronto, Ontario M5S 1A8, Canada.
Young
Alan J
AJ
Dudler
Lisbet
L
Cahill
Ross N P
RN
Kimpton
Wayne
W
Washington
Elizabeth
E
Hay
John B
JB
D000818:Animals; D003713:Dendritic Cells; D005260:Female; D005434:Flow Cytometry; D005452:Fluoresceins; D007962:Leukocytes; D051381:Rats; D012756:Sheep; D013194:Staining and Labeling / Q000379:methods*; D013388:Succinimides
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D013388:Succinimides