[Experimental study on xiaoliu pingyi mixture with medicated serum in inducing apoptosis of human hepatocellular carcinoma cell line H-7402].
OBJECTIVE
To observe the efficacy of Xiaoliu Pingyi Mixture (XLPY) in inducing the apoptosis of human hepatocellular carcinoma (HCC) cell line H-7402.


METHODS
Using serum pharmacologic method, the morphological change of apoptotic cells was observed at different time points by co-culturing with different concentrations of medicated serum containing XLPY with light and fluorescent microscopy, flow cytometry, and immunohistochemical staining methods. Blank serum was taken as control.


RESULTS
The different concentration of medicated serum containing XLPY could significantly inhibit the growth of H-7402 cell line in vitro. After treated with medicated serum of XLPY, the cellular morphologic changes were typical apoptosis under light and fluorescent microscopy, the sub-G1 peaks were observed by flow cytometric assays. The apoptosis rate revealed a medicated serum concentration and time dependent manner. The control group 5%, 10% medicated serum induced apoptotic rate were 0.49%, 5.58% and 8.46% by 24 h; while by 48 h, it was 0.83%, 11.58% and 14.25% respectively. bcl-2 gene examination showed that the bcl-2 gene expression was inhibited.


CONCLUSIONS
XLPY could induce apoptosis in carcinoma cells, and play the clinical role of anti-tumor and anti-metastasis.
Li
X R
XR
Affiliated Hospital of Shandong TCM University, Jinan 250011.
Zhang
D
D
Qi
Y F
YF
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D018920:Coculture Techniques; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal