[Effect of tiaoxin recipe on learning and memory related gene expression in hippocampus of senescence accelerated mice].
OBJECTIVE
To study the effect of Tiaoxin Recipe (TXR) on learning and memory related gene expression in hippocampus of senescence accelerated mice (SAM).


METHODS
Changes of learning and memory related gene expression, including mineralocorticoid receptor (MR), presenile protein 1 and 2 (PS-1, PS-2), tau, APP, apoE and bcl-2 in hippocampus of SAM were determined by reverse transcription polymerase chain reaction (RT-PCR). The effect of TXR were tested. E2020 was used as the drug for control.


RESULTS
Compared with those in the same aged mice, in the 5-month old SAM, levels of gene expression of MR, tau, PS-2 and APP were significantly higher, that of apo-E lower, levels of gene expression PS-1 and bcl-2 were unobviously changed; while in the 12-month old SAM, gene expression of MR and tau were higher, bcl-2 was lower and PS-1, PS-2, apoE and APP were also unobviously changed. Continuously orally taken TXR could correct the abnormality of MR, tau and apoE gene expression in hippocampus of 5-month SAM and that of MR and bcl-2 in 12-month SAM.


CONCLUSIONS
Continuously orally taken of TXR has the effect of regulating and correcting learning and memory related gene expression in hippocampus of 5-month and 12-month SAM.
Zhou
Wen-xia
WX
Institute of Pharmacology and Toxicology, Military Medical Academy of PLA, Beijing 100850.
Zhang
Yong-xiang
YX
D000375:Aging / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001057:Apolipoproteins E / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D006624:Hippocampus / Q000378:metabolism*; D007858:Learning / Q000187:drug effects; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008568:Memory / Q000187:drug effects*; D051379:Mice; D053764:Presenilin-1; D053766:Presenilin-2; D016875:tau Proteins / Q000096:biosynthesis* / Q000235:genetics
D001057:Apolipoproteins E; D008565:Membrane Proteins; D053764:Presenilin-1; D053766:Presenilin-2; D016875:tau Proteins