title: [Evaluation of Schistosoma japonicum tegumental membrane antigen (SjMAg) in immunodiagnosis and efficacy assessment].
abstract: OBJECTIVE
  To evaluate the specificity and sensitivity of SjMAg in detecting specific antibodies in sera of patients with schistosomiasis japonica and in assessing therapeutic efficacy.
  
  
  METHODS
  SjMAg-ELISA was used to determine the specific IgG and IgG4 in sera of patients with schistosomiasis japonica at different time points after chemotherapy.
  
  
  RESULTS
  SjMAg-ELISA was as sensitive and specific as SEA-ELISA. The negative conversion rate of IgG detected by SjMAg-ELISA 12 months after treatment was 80.0%, being significantly higher than 43.3% by SEA-ELISA. The negative conversion rate of IgG4 detected by SjMAg-ELISA 12 months after treatment was 93.3%, being significantly higher than 60.0% by SEA-ELISA. The negative conversion rates of IgG and IgG4 2 yr after treatment were 92.9% and 97.6% respectively.
  
  
  CONCLUSIONS
  SjMAg-ELISA is comparable to SEA-ELISA in diagnosing schistosomiasis japonica and is more effective in assessing therapeutic efficacy.
authors.affiliation: Hunan Medical University, Changsha 410078.
authors.forename: S K
authors.initials: SK
authors.lastname: Zhang
authors.forename: X Y
authors.initials: XY
authors.lastname: Yi
authors.forename: X H
authors.initials: XH
authors.lastname: Shu
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Li
authors.forename: Q R
authors.initials: QR
authors.lastname: Zeng
authors.forename: J C
authors.initials: JC
authors.lastname: Zhou
authors.forename: X F
authors.initials: XF
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis*; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G