[Study on genomic diversity among different isolates of Schistosoma japonicum in China].
OBJECTIVE
To study genomic diversity among the isolates of Schistosoma japonicum in China.


METHODS
Schistosome adults were collected from different endemic areas. Mitochondrial NADH dehydrogenase 1 (ND1) and cytochrome C oxidase 1 (CO1) gene fragments of the worms were amplified by PCR, cloned into plasmid, and finally sequenced. Program MEGA was used for sequence analysis.


RESULTS
The ND1 and CO1 genes amplified from different geographic strains were 476 bp and 1,033 bp, respectively. There are two distinct haplotypes, type I and II, for both ND1 and CO1 nucleotide sequences. The differences between the two types were 4.0% and 3.4%. They are considered to be two different genetypes by the phylogenetic analysis. In individual mitochondrial gene, type I of ND1 was fixedly accompanied with type I of CO1 gene, and type II of ND1 with type II of CO1.


CONCLUSIONS
There are two different genetypes of ND1 and CO1 genes of S. japonicum in China.
Qiu
Chi-ping
CP
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
Chris
Spolsky
S
Xia
Ming-yi
MY
Chen
Qin
Q
Tong
Xiao-mei
XM
Gong
Xin
X
Davis
George
G
Feng
Zheng
Z
D000818:Animals; D001483:Base Sequence; D002681:China; D003576:Electron Transport Complex IV / Q000235:genetics*; D005838:Genotype; D006239:Haplotypes; D051379:Mice; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D010802:Phylogeny; D012549:Schistosoma japonicum / Q000201:enzymology / Q000235:genetics*
D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV