Influence of prednisolone on the secretion of mucin from the HT29-MTX cell line.
Glucocorticoids have been used in the treatment of otitis media with effusion with promising but inconsistent results. The HT29-MTX cell line is a completely differentiated and almost exclusively mucus-secreting goblet cell line. To assess the potential of steroids in suppressing mucin secretion, we have studied the response of this cell culture to prednisolone. Confluent cell cultures were trypsinized, subcultured in six-well plates and incubated with five doses of prednisolone from 10-3 M to 10-11 M and over a varying time course from 6 to 36 h. Analysis was performed using a monoclonal mouse antibody to human gastric mucin by dot-blot ELISA. Prednisolone caused a consistent reduction in mucin production from this cell line. Increasing concentrations of prednisolone resulted in increasing suppression of MUC5AC secretion. There is a dose-dependent suppression of mucin secretion by prednisolone, with a maximum effect of 21% over control seen at the highest steroid concentration used.
Fergie
N
N
Department of Otolaryngology and Head and Neck Surgery, Queen's Medical Centre, Nottingham, UK. neil.fergie@virgin.net
Guo
L
L
Sithole
J
J
Pearson
J P
JP
Birchall
J P
JP
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D004797:Enzyme-Linked Immunosorbent Assay; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D020397:Goblet Cells / Q000378:metabolism / Q000473:pathology; D019073:HT29 Cells / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D009077:Mucins / Q000378:metabolism*; D010034:Otitis Media with Effusion / Q000378:metabolism / Q000473:pathology; D011239:Prednisolone / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D000911:Antibodies, Monoclonal; D009077:Mucins; D011239:Prednisolone