title: A simple method of DNA extraction and STR typing from urine samples using a commercially available DNA/RNA extraction kit.
abstract: We devised a simple DNA extraction procedure suitable for STR typing of urine sample. Use of a commercially available DNA/RNA extraction kit equipped with a silica-gel-based membrane made it possible to omit the recovery of urinary nucleated cells by sedimentation before the extraction. Successful genotyping of the TH01, HumTPO and multiplex STRs was achieved using aliquots of urine as small as 100 microL. Furthermore, application of this DNA extraction procedure to frozen urine samples provided STR allele results comparable to results obtained from fresh samples. Therefore, this extraction procedure is considered to be effective for STR typing of urine samples in both the frozen and aqueous state. Furthermore, addition of sodium azide to fresh urine samples prolonged their storage duration even at room temperature.
authors.affiliation: Department of Biology, Fukui Medical University, Fukui, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Yasuda
authors.forename: Reiko
authors.initials: R
authors.lastname: Iida
authors.forename: Haruo
authors.initials: H
authors.lastname: Takeshita
authors.forename: Misuzu
authors.initials: M
authors.lastname: Ueki
authors.forename: Tamiko
authors.initials: T
authors.lastname: Nakajima
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kaneko
authors.forename: Kouichi
authors.initials: K
authors.lastname: Mogi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tsukahara
authors.forename: Koichiro
authors.initials: K
authors.lastname: Kishi
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods; D005838:Genotype; D006801:Humans; D011933:Reagent Kits, Diagnostic; D020080:Tandem Repeat Sequences; D016482:Urinalysis
chemical_list: D011933:Reagent Kits, Diagnostic; D004247:DNA