title: [Construction and characterization of the cDNA library from Schistosoma japonicum cercariae].
abstract: OBJECTIVE
  To construct a cDNA library from Schistosoma japonicum cercariae.
  
  
  METHODS
  Total RNA was extracted from 500,000 S. japonicum cercariae and used to synthesize the double-stranded cDNA and cloned into the TriplEx2 vector using "SMART cDNA Construction Kit".
  
  
  RESULTS
  The primary titer of the constructed cDNA library is 1.8 x 10(7) pfu/ml and the titer of amplified library is 2.5 x 10(10) pfu/ml. The average size of inserts is 1.075 kb. The recombinant efficiency is 94.4%. The full length cDNA of S. japonicum TPI and JF-2 genes was successfully amplified from the library.
  
  
  CONCLUSIONS
  A cDNA library of S. japonicum cercariae has been constructed successfully.
authors.affiliation: Department of Parasitology, Institute of Tropical Medicine, First Military Medical University, Guangzhou 510515.
authors.forename: Xiao-guang
authors.initials: XG
authors.lastname: Chen
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Hong-juan
authors.initials: HJ
authors.lastname: Peng
authors.forename: Shu-man
authors.initials: SM
authors.lastname: Shen
authors.forename: Ming-chiu
authors.initials: MC
authors.lastname: Fung
mesh_terms: D000818:Animals; D015723:Gene Library; D012549:Schistosoma japonicum / Q000235:genetics*