[A study on the recombinant 26 kDa glutathione-S-transferase as a vaccine candidate: dynamics of antibodies in immunized buffaloes and protection against Schistosoma japonicum infections].
OBJECTIVE
To observe the dynamics of antibodies and protection against Schistosoma japonicum infections in buffaloes after immunized with recombinant 26 kDa glutathione-S-transferase (reSjc26GST).


METHODS
Buffaloes in 2 villages endemic for schistosomiasis japonica were selected as test and control groups, respectively. In test group initially 96 buffaloes were vaccinated with reSjc26GST, and 90 buffaloes in the control group did not experience vaccination. The indicators included levels of antibodies to reSjc26GST in buffaloes before and after infection with S. japonicum and changes in infection rate.


RESULTS
Specific antibodies, which showed a trend of trapezoid increase, were induced in buffaloes after immunized with reSjc26GST. Twenty months after immunization, the infection rate of the test group was decreased by 62.2% when compared with that before vaccination, and by 67.7% when compared with that of the control in the corresponding period.


CONCLUSIONS
Specific antibodies and a certain extent of protection were induced in buffaloes after immunized with reSjc26GST, which played an significant role in ameliorating morbidity.
He
Y K
YK
Hunan Institute of Parasitic Diseases, Yueyang 414000.
Song
G C
GC
Liu
S X
SX
Luo
X S
XS
Zhang
X Y
XY
Xu
Y X
YX
Yu
X L
XL
Yang
R Q
RQ
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D002020:Buffaloes; D005982:Glutathione Transferase / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012549:Schistosoma japonicum / Q000201:enzymology / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary; D014614:Vaccines, Synthetic
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D005982:Glutathione Transferase