Serum antibodies to lipophilin B detected in late stage breast cancer patients.
Lipophilin B mRNA is overexpressed in approximately 70% of breast tumors and shows a high degree of correlation with the mRNA expression profile of mammaglobin. This is further supported by the recent finding that, like other members of the secretoglobulin-uteroglobin family, mammaglobin and lipophilin B form a heteroduplex. The studies described show that there are pre-existing antibodies to lipophilin B peptide in the sera of breast cancer patients with different stages and grade of tumor and that this response is different from that seen to recombinant mammaglobin and native mammaglobin-lipophilin B complex. The highest titers were observed in later stage tumors. In addition, low levels of antibody were also seen in some patients with prostate and ovarian cancers, consistent with lipophilin B mRNA expression in these tumors at lower abundance than in breast tumors. In contrast, lipophilin B antibodies were absent in 20 healthy donor sera and 30 lung cancer sera. A polymorphism identified in Lipophilin B did not appear to influence human sera reactivity. The data indicate that humoral immune responses to lipophilin B may serve as a diagnostic indicator, particularly for breast cancer.
Carter
Darrick
D
Department of Tumor Antigen Discovery, Corixa Corporation, Seattle, Washington 98104, USA.
Dillon
Davin C
DC
Reynolds
Lisa D
LD
Retter
Marc W
MW
Fanger
Gary
G
Molesh
David A
DA
Sleath
Paul R
PR
McNeill
Patricia D
PD
Vedvick
Thomas S
TS
Reed
Steven G
SG
Persing
David H
DH
Houghton
Raymond L
RL
D000595:Amino Acid Sequence; D000906:Antibodies / Q000097:blood*; D000918:Antibody Specificity; D001943:Breast Neoplasms / Q000097:blood / Q000276:immunology* / Q000473:pathology; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D018450:Disease Progression; D016889:Endometrial Neoplasms / Q000097:blood / Q000276:immunology; D005260:Female; D005914:Globins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D009185:Myelin Proteins; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000097:blood / Q000276:immunology; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000097:blood / Q000276:immunology; D011510:Proteolipids; D012016:Reference Values; D060765:Secretoglobins; D014158:Transcription, Genetic; D014598:Uteroglobin
D000906:Antibodies; D002352:Carrier Proteins; D009185:Myelin Proteins; D010446:Peptide Fragments; D011510:Proteolipids; C556464:SCGB1D2 protein, human; D060765:Secretoglobins; D005914:Globins; D014598:Uteroglobin