[Study on the technique of Rehmannia glutinosa virus-free].
The stem tip from germ-free stem segment of Rehmannia glutinosa cultured in test tube can be induced into virus-free seedling. The experiment showed that the proper disinfectant for stem segments of Rehmannia glutinosa was 0.05% HgCl2. The seedings from stem segments grew better with MS in the concentration of agar 0.7% and pH7. The stem tips could be directly induced to seedlings by using MS + 6-BA(0.05 mg/L). The MS media for seedlings virus-free culture are 1/4 macro-elements + 1/2 micro-elements and using edible sugar instead of sucrose, so the cost of media could be decreased to 48.7%.
Wu
Zongxin
Z
Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng, Shanxi 044000.
Xie
Honge
H
Feng
Wenlong
W
Chen
Li
L
Li
Hongxia
H
Li
Jianghui
J
D046508:Culture Techniques; D004202:Disinfectants / Q000494:pharmacology; D008627:Mercuric Chloride / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D010942:Plant Viruses / Q000187:drug effects; D010946:Plants, Medicinal / Q000254:growth & development* / Q000821:virology; D032267:Rehmannia / Q000254:growth & development* / Q000821:virology; D036226:Seedlings / Q000254:growth & development / Q000821:virology
D004202:Disinfectants; D010937:Plant Growth Regulators; D008627:Mercuric Chloride