title: Decreased intercellular adhesion molecule-1 (CD54) and L-selectin (CD62L) expression on peripheral blood natural killer cells in asthmatic children with acute exacerbation.
abstract: BACKGROUND
  The capacity of inflammatory cells to adhere involves an array of adhesion molecules, and is critical to the inflammatory responses seen in childhood asthma. We aimed to determine the changes of intercellular adhesion molecule-1 (ICAM-1) and L-selectin expressed on peripheral blood (PB) T lymphocytes and natural killer (NK) cells in asthmatic children with acute exacerbation and after prednisolone therapy.
  
  
  METHODS
  Flow cytometric analysis was performed to determine the expression of ICAM-1 (CD54) and L-selectin (CD62L) on T (CD3+) cells and NK (CD3-/CD56+) cells of PB from children with allergic asthma with acute exacerbation and in a stable condition after prednisolone therapy. Atopic subjects without asthma and age-matched controls were also included for comparison.
  
  
  RESULTS
  Percentages of PB non-CD3, CD56+ NK cells, but not CD3+ T cells, increased in asthmatic children with acute exacerbation, compared to those assessed in a stable condition after a course of prednisolone. However, significant decrease of ICAM-1 (P = 0.01) and L-selectin (P = 0.01) expression on PB NK cells, but not on T cells, were found in children with acute asthma compared to those in a stable condition. NK cells in children with acute asthma showed minimal expression of CD69 and CD25.
  
  
  CONCLUSIONS
  Results suggests that either NK cells expressing ICAM-1 and L-selectin selectively migrated into inflamed lung tissues, or subsets of NK cells not expressing ICAM-1/L-selectin were expanded during acute exacerbation of childhood asthma.
authors.affiliation: Division of Allergy, Asthma and Rheumatology, Chang Gung Children's Hospital, Taoyuan, Taiwan.
authors.forename: S-J
authors.initials: SJ
authors.lastname: Lin
authors.forename: L-Y
authors.initials: LY
authors.lastname: Chang
authors.forename: D-C
authors.initials: DC
authors.lastname: Yan
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Huang
authors.forename: T-J
authors.initials: TJ
authors.lastname: Lin
authors.forename: T-Y
authors.initials: TY
authors.lastname: Lin
mesh_terms: D000208:Acute Disease; D015703:Antigens, CD / Q000096:biosynthesis / Q000097:blood / Q000187:drug effects; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis / Q000097:blood / Q000187:drug effects; D001249:Asthma / Q000097:blood / Q000188:drug therapy / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D017252:CD3 Complex / Q000096:biosynthesis / Q000097:blood / Q000187:drug effects; D002648:Child; D002669:Child Welfare; D002675:Child, Preschool; D005938:Glucocorticoids / Q000627:therapeutic use; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000097:blood* / Q000187:drug effects; D007694:Killer Cells, Natural / Q000187:drug effects / Q000378:metabolism*; D019041:L-Selectin / Q000096:biosynthesis* / Q000097:blood* / Q000187:drug effects; D037181:Lectins, C-Type; D011239:Prednisolone / Q000627:therapeutic use; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis / Q000097:blood / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D013624:Taiwan; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D015415:Biomarkers; D017252:CD3 Complex; D005938:Glucocorticoids; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin; D037181:Lectins, C-Type; D011239:Prednisolone; D015375:Receptors, Interleukin-2; D000099192:CD69 Antigens