Siderophore production by the magnetic bacterium Magnetospirillum magneticum AMB-1.
Siderophore production by the magnetic bacterium Magnetospirillum magneticum AMB-1 is elicited by sufficient iron rather than by iron starvation. In order to clarify this unusual pattern, siderophore production was monitored in parallel to iron assimilation using the chrome azurol sulfonate assay and the ferrozine method respectively. Iron concentration lowered approximately five times less than its initial concentration only within 4 h post-inoculation, rendering the medium iron deficient. A concentration of at least 6 microM Fe(3+) is required to initiate siderophore production. The propensity of M. magneticum AMB-1 for the assimilation of large amounts of iron accounts for the rapid depletion of iron in the medium, thereby triggering siderophore excretion. M. magneticum AMB-1 produces both hydroxamate and catechol siderophores.
Calugay
Ronie J
RJ
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-Cho, Koganei, Tokyo, Japan.
Miyashita
Hideaki
H
Okamura
Yoshiko
Y
Matsunaga
Tadashi
T
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D002396:Catechols / Q000378:metabolism; D003470:Culture Media; D006877:Hydroxamic Acids / Q000378:metabolism; D008280:Magnetics; D012244:Rhodospirillaceae / Q000378:metabolism*; D017262:Siderophores / Q000032:analysis / Q000096:biosynthesis*
D001426:Bacterial Proteins; D002396:Catechols; D003470:Culture Media; D006877:Hydroxamic Acids; D017262:Siderophores; C034221:catechol