title: Motions of single molecules and proteins in trehalose glass.
abstract: The fluorescence intensity-time records of individual metal-free porphyrin cytochrome-c and Zn porphyrin cytochrome-c molecules whose translational motions are restricted by encapsulation in trehalose are examined by single-molecule spectroscopy by means of a two-channel confocal microscope that records transient fluorescence signals in two orthogonal polarization directions. Large angular motions often occur on time scales ranging to many seconds. Measurements of the photobleaching time distributions indicate that the trehalose glass restricts the accessibility of the fluorescent molecules to oxygen.
authors.affiliation: Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Erwen
authors.initials: E
authors.lastname: Mei
authors.forename: Jianyong
authors.initials: J
authors.lastname: Tang
authors.forename: Jane M
authors.initials: JM
authors.lastname: Vanderkooi
authors.forename: Robin M
authors.initials: RM
authors.lastname: Hochstrasser
mesh_terms: D003574:Cytochrome c Group / Q000737:chemistry*; D045304:Cytochromes c; D005898:Glass / Q000737:chemistry; D018613:Microscopy, Confocal; D013050:Spectrometry, Fluorescence; D014199:Trehalose / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry
chemical_list: D003574:Cytochrome c Group; C050095:zinc cytochrome c; D045304:Cytochromes c; D014199:Trehalose; D015032:Zinc