[Cellular culture of osteoblasts and fibroblasts on porous calcium-phosphate bone substitutes].
OBJECTIVE
Calcium phosphate ceramics are synthetic bone substitutes able to fill in bone destruction as a support of the bone growth. This work consisted in an in vitro assessment of osteoblasts and fibroblasts cultures on macroporous calcium-phosphate bone substitutes to analyze the interaction between cells and bone substitute.


METHODS
The macroporous ceramic was composed of 70% hydroxyapatite and 30% tri-calcium phosphate with known mechanical and physico-chemical properties. Three compounds were processed with different size of macropore and with or without microporosity on their surface. Cells were seeded on discs measuring 10 mm in diameter and 2 mm in thickness. Cellular viability was evaluated by the MTT test for every stage of observation. An histological study to observe the invasion in the depth of discs was performed. Scanning electron microscopy was used to analyze the cellular comportment in contact with the surface of substitutes.


RESULTS
An exponential cellular growth was effective on each substitute with the two cellular types. Cells spread on the surface of the compounds covering macropores and colonized the depth of the discs. A size of macropore of 300 microm or more seemed to support this invasion. 15 microm sized interconnections appeared to be effective to allow cell migration between macropores. The cell proliferation was similar on substitutes with or without microporosity.


CONCLUSIONS
Biomaterials currently used as bone substitute are more or less osteoconductive but they have no osteoinductive property. A hybrid association of calcium-phosphate ceramic with osteogenic cells should promote the development of a calcium phosphate compound with osteoinductive capacity.
Chouteau
J
J
Laboratoire Mecal, Faculté de Médecine Lyon Sud, 69600 Oullins. juchout@club-internet.fr
Bignon
A
A
Chavassieux
P
P
Chevalier
J
J
Melin
M
M
Fantozzi
G
G
Boivin
G
G
Hartmann
D
D
Carret
J-P
JP
D001672:Biocompatible Materials / Q000737:chemistry / Q000592:standards*; D018786:Bone Substitutes / Q000737:chemistry / Q000592:standards*; D002130:Calcium Phosphates / Q000737:chemistry / Q000592:standards*; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D017886:Durapatite / Q000737:chemistry / Q000592:standards*; D005347:Fibroblasts / Q000166:cytology* / Q000648:ultrastructure; D006801:Humans; D008422:Materials Testing; D016348:Osseointegration; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure; D016062:Porosity
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D017886:Durapatite; C018392:tricalcium phosphate