Capillary electrophoretic separation of enantiomers in a high-pH background electrolyte by means of the single-isomer chiral resolving agent octa(6-O-sulfo)-gamma-cyclodextrin.
The new, alkali-stable, single-isomer, sulfated gamma-cyclodextrin, the sodium salt of octa(6-O-sulfo)-gamma-cyclodextrin (OS) was used for the first time to separate the enantiomers of non-ionic, acidic, basic and ampholytic analytes by capillary electrophoresis in high-pH aqueous background electrolytes. The effective mobilities and separation selectivities were found to follow trends similar to those observed earlier in acidic aqueous background electrolytes. OS proved to be a broadly applicable chiral resolving agent and afforded adequate peak resolution values with short separation times for a number of non-ionic, weak acid, weak base and ampholytic analytes.
Zhu
Wenhong
W
Department of Chemistry, MS 3255, Texas A&M University, College Station, TX 77842-3012, USA.
Vigh
Gyula
G
D004573:Electrolytes / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013237:Stereoisomerism
D004573:Electrolytes