[Determination of IgG content of IVIG by improved rocket immune electrophoresis].
OBJECTIVE
To establish a specific method for determination of IgG in IVIG.


METHODS
The IVIG samples were disposed by acylation and analyzed by Rocket Immunoelectrophoresis. The amount of the IgG in IVIG was calculated by an IgG standard curve.


RESULTS
The standard curve by this method has a very good linear relationship: r = 0.9967. The reproducibilities with 3 bathes of IVIG samples (n = 6) are 5.37% +/- 0.21%, 5.28% +/- 0.39% and 5.80% +/- 0.30%.


CONCLUSIONS
This method could be adapted to regular assay in blood products.
Huang
Fengqiong
F
Chengdu Institute of Biological Products, Chengdu 610063, China.
Chen
Xiaodong
X
Li
Kexi
K
Mao
Xiuzhen
X
Zou
Chengchuan
C
D007122:Immunoelectrophoresis / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis*; D016756:Immunoglobulins, Intravenous / Q000737:chemistry*
D007074:Immunoglobulin G; D016756:Immunoglobulins, Intravenous