Detection of a processed pseudogene of the human MBL-associated serine protease, MASP1.
Southern hybridization analysis of the MASP1 gene using an intron-specific probe detected a single band. An exon-specific probe detected several bands. PCR of genomic DNA using several exon-specific primer sets of MASP1 produced short and long products. Sequence of the shorter products corresponded to the processed pseudogene of MASP1. By fluorescence in situ hybridization, this pseudogene (MASP1P1) was mapped to 1p34.
Mochihara
Y
Y
Sumikin Bio-Science, Inc., Sagamihara-shi, Kanagawa, Japan. mochihara@sumikin-bio.co.jp
Tazawa
H
H
Habuta
S
S
Ohthubo
K
K
Kozaki
T
T
Ishihara
Y
Y
Takayama
Y
Y
Kawakami
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D050606:Mannose-Binding Protein-Associated Serine Proteases; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011544:Pseudogenes; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; C084067:MASP1 protein, human; D050606:Mannose-Binding Protein-Associated Serine Proteases; D012697:Serine Endopeptidases