In vitro effects of ciprofloxacin and roxithromycin on apoptosis of jurkat T lymphocytes.
Ciprofloxacin (CPFX) and roxithromycin (RXM) induced apoptosis of activated Jurkat T cells in vitro. CPFX showed concentration-dependent acceleration of apoptosis of activated Jurkat T cells by enhancing the expression of FasL and activities of caspase-3 and -8. RXM accelerated cell death, enhanced expression of FasL and caspase-3 but not caspase-8, and did not show the concentration dependency.
Jun
Yong-Taek
YT
Division of Infectious Diseases, Department of Internal Medicine, College of Medicine, Catholic University of Korea, Seoul, Korea.
Kim
Hee-Jung
HJ
Song
Min-Jin
MJ
Lim
Ji-Hyang
JH
Lee
Dong-Gun
DG
Han
Kyung-Ja
KJ
Choi
Su-Mi
SM
Yoo
Jin-Hong
JH
Shin
Wan-Shik
WS
Choi
Jung-Hyun
JH
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000096:biosynthesis; D002470:Cell Survival / Q000187:drug effects; D002939:Ciprofloxacin / Q000494:pharmacology*; D053938:DNA Fragmentation; D004797:Enzyme-Linked Immunosorbent Assay; D053222:Fas Ligand Protein; D016650:Fluorescein-5-isothiocyanate; D005455:Fluorescent Antibody Technique; D019254:Genes, bcl-2 / Q000235:genetics; D006801:Humans; D019169:Jurkat Cells; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2; D015575:Roxithromycin / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D051028:bcl-2-Associated X Protein; D019014:fas Receptor / Q000096:biosynthesis
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D019014:fas Receptor; D015575:Roxithromycin; D002939:Ciprofloxacin; D020169:Caspases; D016650:Fluorescein-5-isothiocyanate