Tumor initiating activity of Helicobacter pylori water extract on mouse skin carcinogenesis.
Helicobacter pylori (H. pylori) infection has been associated with gastric carcinogenesis, but responsible and detail mechanisms are insufficient by the absence of adequate data. To obtain direct evidence regarding the carcinogenicity of H. pylori, we investigated the initiating and promoting activity of H. pylori water extract (HPE) in two-stage mouse skin carcinogenesis model. HPE treatment, as an initiation, significantly enhanced tumor formation compared with control group. Moreover, HPE treatment increased production of 8-hydroxydeoxyguanosine in epidermal cells and HPE-initiated/TPA-promoted papillomas demonstrated a point mutation of the Ha-ras gene. These results suggest an initiating activity of HPE on two-stage mouse skin carcinogenesis.
Ishikawa
Takeshi
T
First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
Yoshida
Norimasa
N
Tokuda
Harukuni
H
Ichiishi
Eiichiro
E
Kuchide
Masashi
M
Kokura
Satoshi
S
Naito
Yuji
Y
Toyokuni
Shinya
S
Nishino
Hoyoku
H
Yoshikawa
Toshikazu
T
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000483:Alleles; D000818:Animals; D002273:Carcinogens / Q000302:isolation & purification / Q000633:toxicity*; D002458:Cell Fractionation; D003043:Cocarcinogenesis; D003062:Codon / Q000187:drug effects; D004247:DNA / Q000187:drug effects / Q000235:genetics; D018736:DNA Adducts / Q000032:analysis; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D018450:Disease Progression; D005260:Female; D011905:Genes, ras / Q000187:drug effects; D016480:Helicobacter pylori / Q000737:chemistry*; D008163:Luminescent Measurements; D051379:Mice; D018632:Mice, Inbred SENCAR; D018384:Oxidative Stress; D010212:Papilloma / Q000139:chemically induced* / Q000235:genetics; D009195:Peroxidase / Q000032:analysis; D017354:Point Mutation; D012867:Skin / Q000187:drug effects / Q000201:enzymology; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000235:genetics; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity; D014867:Water
D002273:Carcinogens; D003062:Codon; D018736:DNA Adducts; D014867:Water; D013481:Superoxides; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D009195:Peroxidase; D003849:Deoxyguanosine; D013755:Tetradecanoylphorbol Acetate