[The in situ hybridization effort estimation between antisense RNA probe and random primed DNA probe].
OBJECTIVE
To estimate the effort between antisense RNA probe and random primed DNA probe.


METHODS
The in situ hybridization was conducted on parafin section from wounding model of rat skin.


RESULTS
Although both probes appeared positive staining, RNA probes was superior to DNA probes in terms of depth of staining and background.


CONCLUSIONS
RNA probe showed more satisfactorily on ISH.
Liu
Ning-guo
NG
Institution of Forensic Sciences of Ministry of Justice, Shanghai 200063. liuningguo@163.net
Zhao
Zi-qin
ZQ
Gu
Yun-ju
YJ
D000818:Animals; D016373:DNA, Antisense; D004076:Digoxigenin; D005353:Fibronectins / Q000096:biosynthesis* / Q000235:genetics; D017403:In Situ Hybridization / Q000379:methods*; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries / Q000378:metabolism* / Q000473:pathology
D016373:DNA, Antisense; D005353:Fibronectins; D015347:RNA Probes; D012333:RNA, Messenger; D004076:Digoxigenin