Characterization of an interleukin-7-dependent thymic cell line derived from a p53(-/-) mouse.
In order to study the response of T cells to IL-7, we aimed to generate an IL-7-dependent thymocyte line. CD4(-)CD8(-) thymocytes from a p53(-)/(-) mouse were continuously propagated in interleukin-7 (IL-7), and after 2 months there developed an immortal line termed "D1." The D1 line has retained a stable dependency on IL-7. Withdrawal of IL-7 from D1 cells induced arrest in G1 phase of the cell cycle, followed by apoptosis. In addition to IL-7, several other cytokines that employ gamma(c) as part of their receptor were also capable of stimulating D1 cell survival and proliferation. Gene induction by IL-7 was analyzed in D1 cells using RNase protection and array analysis and revealed a number of transcripts potentially involved in cell cycle, apoptosis and signaling.
Kim
Kyungjae
K
Laboratory of Molecular Immunoregulation, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA.
Khaled
Annette R
AR
Reynolds
Della
D
Young
Howard A
HA
Lee
Chong-Kil
CK
Durum
Scott K
SK
D000818:Animals; D017209:Apoptosis; D002453:Cell Cycle; D002460:Cell Line; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D016158:Genes, p53; D015851:Interleukin-7 / Q000494:pharmacology*; D051379:Mice; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology*
D015851:Interleukin-7; D012333:RNA, Messenger