title: Development of an in vitro method for the evaluation of candidate repellents against Leptotrombidium (Acari: Trombiculidae) chiggers.
abstract: We developed a rapid and economical in vitro procedure with which to evaluate the efficacy of candidate repellents against chiggers. The procedure requires only 5 min and a small number of chiggers to obtain a valid estimate of the median effective dose. We used this procedure to evaluate the repellent activity of 11 compounds against the chigger, Leptotrombidium imphalum Vercammen-Grandjean and Langston. Median effective doses were determined for 10 of the 11 compounds. DM-165-2 (N,N-diethyl-3-flurobenzamide) was the only compound that was significantly more effective than deet.
authors.affiliation: Department of Entomology, US Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
authors.forename: Kriangkrai
authors.initials: K
authors.lastname: Lerdthusnee
authors.forename: Nittaya
authors.initials: N
authors.lastname: Khlaimanee
authors.forename: Taweesak
authors.initials: T
authors.lastname: Monkanna
authors.forename: Siriporn
authors.initials: S
authors.lastname: Mungviriya
authors.forename: Warisa
authors.initials: W
authors.lastname: Leepitakrat
authors.forename: Mustapha
authors.initials: M
authors.lastname: Debboun
authors.forename: Russell E
authors.initials: RE
authors.lastname: Coleman
mesh_terms: D000818:Animals; D001179:Arthropod Vectors; D003671:DEET / Q000633:toxicity; D007302:Insect Repellents / Q000633:toxicity*; D013329:Structure-Activity Relationship; D014324:Trombiculidae / Q000187:drug effects*
chemical_list: D007302:Insect Repellents; D003671:DEET