Inactivation of classical swine fever virus: association of hydrostatic pressure and ultraviolet irradiation.
Reversible pressure-induced disassembly of several viruses has suggested the idea of using hydrostatic pressure to suppress virus infectivity. In this study, the effects of high hydrostatic pressure and ultraviolet (UV) irradiation were investigated on classical swine fever virus (CSFV) in an attempt to eliminate residual infectivity. The structural modifications were followed by intrinsic fluorescence and biological activity assays. The kinetics of CSFV inactivation showed that pressure-induced inactivation was not enough to eliminate viral infectivity. However, when pressure was applied in association with UV irradiation no infectious focus was observed. The application of these two methods against CSFV can be an attractive inactivation strategy for the development of a vaccine.
Freitas
T R P
TR
Departamento de Virologia, Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, 21941-590, Rio de Janeiro RJ, Brazil.
Gaspar
L P
LP
Caldas
L A
LA
Silva
J L
JL
Rebello
M A
MA
D000818:Animals; D002460:Cell Line; D006691:Classical Swine Fever / Q000517:prevention & control; D006692:Classical Swine Fever Virus / Q000302:isolation & purification / Q000472:pathogenicity / Q000528:radiation effects*; D006874:Hydrostatic Pressure; D066298:In Vitro Techniques; D007700:Kinetics; D008027:Light; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D034421:Sus scrofa; D014466:Ultraviolet Rays; D014613:Vaccines, Attenuated / Q000302:isolation & purification; D014765:Viral Vaccines / Q000302:isolation & purification; D038301:Virus Inactivation / Q000528:radiation effects
D014613:Vaccines, Attenuated; D014765:Viral Vaccines