title: Structural and biological effects of a beta2- or beta3-amino acid insertion in a peptide.
abstract: Molecular mechanics calculations on conformers of Ac-HGly-NHMe, Ac-beta2-HAla-NHMe and Ac-beta3-HAla-NHMe indicate that low-energy conformations of the beta-amino acids backbone, corresponding to gauche rotamers around the Calpha-Cbeta bond, may overlap canonical backbone conformers observed for alpha-amino acids. Therefore, Substance P (SP) was used as a model peptide to analyse the structural and biological consequences of the substitution of Phe7 and Phe8 by (R)-beta2-HPhe and of Gly9 by HGly (R)-beta2-HAla or (S)-beta3-HAla. [(R)-beta2-HAla9]SP has pharmacological potency similar to that of SP while [HGly9]SP and [(S)-beta3-HAla9]SP show a 30- to 50-fold decrease in biological activities. The three analogues modified at position 9 are more resistant to degradation by angiotensin converting enzyme than SP and [Ala9]SP. NMR analysis of these SP analogues suggest that a beta-amino acid insertion in position 9 does not affect the overall backbone conformation. Altogether these data suggest that [HGly9]SP, [(S)-beta3-HAla9]SP and [(R)-beta2-HAla9]SP could adopt backbone conformations similar to that of SP, [Ala9]SP and [Pro9]SP. In contrast, incorporation of beta2-HPhe in position 7 and 8 of SP led to peptides that are almost devoid of biological activity. Thus, a beta-amino acid could replace an alpha-amino acid within the sequence of a bioactive peptide provided that the additional methylene group does not cause steric hindrance and does not confine orientations of the side chain to regions of space different from those permitted in the alpha-amino acid.
authors.affiliation: UMR 7613 CNRS-Paris 6, Université Pierre et Marie Curie, Paris, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Sagan
authors.forename: Thierry
authors.initials: T
authors.lastname: Milcent
authors.forename: Rachel
authors.initials: R
authors.lastname: Ponsinet
authors.forename: Odile
authors.initials: O
authors.lastname: Convert
authors.forename: Olivier
authors.initials: O
authors.lastname: Tasseau
authors.forename: Gérard
authors.initials: G
authors.lastname: Chassaing
authors.forename: Solange
authors.initials: S
authors.lastname: Lavielle
authors.forename: Olivier
authors.initials: O
authors.lastname: Lequin
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D018040:Receptors, Neurokinin-1 / Q000737:chemistry / Q000378:metabolism*; D013373:Substance P / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D018040:Receptors, Neurokinin-1; D013373:Substance P