Crystallization and preliminary X-ray analysis of swine vesicular disease virus (SVDV).
Three different crystal forms of the swine vesicular disease virus (SVDV), isolate SPA/2/'93, were obtained by the hanging-drop vapour-diffusion technique using ammonium sulfate and sodium/potassium phosphate as precipitants. Monoclinic crystals, space group C2, with unit-cell parameters a = 473.7, b = 385.3, c = 472.8 A, beta = 100.4 degrees, contain one virus pArticle in the crystal asymmetric unit and diffract to 3.0 A resolution. A second type of crystals had a cubic morphology and diffracted beyond 2.6 A resolution. These crystals belong to a primitive orthorhombic space group, with unit-cell parameters a = 319.6, b = 353.8, c = 377.7 A, and contain half a virus pArticle in the asymmetric unit. A third type of crystals, with a prismatic shape and belonging to space group I222, was also obtained under similar crystallization conditions. These latter crystals, with unit-cell parameters a = 318.3, b = 349.9, c = 371.7 A, diffract to at least 3.0 A resolution and contain 15 protomers per asymmetric unit; this requires that three perpendicular crystal twofold axes coincide with three of the viral pArticles' dyad axes.
Jimenez-Clavero
Miguel A
MA
Instituto Nacional de Investigaciones Agrarias, Ctra. Coruña Km 7.5, 28040-Madrid, Spain.
Ley
Victoria
V
Fita
Ignacio
I
Verdaguer
Núria
N
D000645:Ammonium Sulfate; D000818:Animals; D002478:Cells, Cultured; D003460:Crystallization; D018360:Crystallography, X-Ray; D029822:Enterovirus B, Human / Q000737:chemistry* / Q000254:growth & development; D007202:Indicators and Reagents; D010710:Phosphates; D013552:Swine
D007202:Indicators and Reagents; D010710:Phosphates; D000645:Ammonium Sulfate