title: Cumulus cells reduce the spermatozoa-zona binding inhibitory activity of human follicular fluid.
abstract: OBJECTIVE
  To investigate the effects of human follicular fluid cultured with cumulus cells to inhibit the binding of spermatozoa to the zona pellucida of oocytes.
  
  
  METHODS
  Controlled experimental laboratory study.
  
  
  METHODS
  University gynecology unit.
  
  
  METHODS
  Women undergoing assisted reproduction program and men visiting the subfertility clinics.
  
  
  METHODS
  Culture medium and human follicular fluid were used to culture cumulus cells in vitro for specified time periods.
  
  
  METHODS
  Zona binding capacity and motility of spermatozoa after incubation with cumulus cells treated culture medium or human follicular fluid.
  
  
  RESULTS
  Compared with the control medium, spent culture media after culturing cumulus cells for 3, 5, and 7 hours did not affect the motility and zona binding capacity of the treated spermatozoa. Significantly more spermatozoa treated with human follicular fluid that had been preincubated with cumulus cells for 5 and 7 hours bound onto hemizona in hemizona binding assay when compared with those preincubated in human follicular fluid without cumulus treatment. The hemizona index increased with the increase in the duration of cumulus cell treatment. Human follicular fluid with or without cumulus cells maintained sperm motility to similar extent for 3 hours.
  
  
  CONCLUSIONS
  Cumulus cells reduced the inhibitory effect of human follicular fluid on spermatozoa-zona binding in vitro in a time-dependent manner.
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Hong Kong, China.
authors.forename: Shun-jia
authors.initials: SJ
authors.lastname: Hong
authors.forename: Jenny Y M
authors.initials: JY
authors.lastname: Tse
authors.forename: Pak-chung
authors.initials: PC
authors.lastname: Ho
authors.forename: William Shu-biu
authors.initials: WS
authors.lastname: Yeung
mesh_terms: D017077:Culture Media, Conditioned; D005260:Female; D015571:Follicular Fluid / Q000502:physiology*; D006801:Humans; D008297:Male; D009865:Oocytes / Q000502:physiology*; D013081:Sperm Motility / Q000502:physiology; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology*
chemical_list: D017077:Culture Media, Conditioned