Capture and detection of a quencher labeled oligonucleotide by poly(phenylene ethynylene) particles.
Fluorescence quenching of poly(phenylene ethynylene) (PPE) particles by a Cy-5 labeled oligonucleotide is 2 orders of magnitude more sensitive than direct excitation of the Cy-5 fluorophore.
Moon
Joong Ho
JH
Nomadics, Inc., 61 Rogers Street, Cambridge, MA 02142, USA.
Deans
Robert
R
Krueger
Elizabeth
E
Hancock
Lawrence F
LF
D018076:DNA, Complementary / Q000032:analysis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D008863:Microspheres; D015345:Oligonucleotide Probes / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D018076:DNA, Complementary; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011108:Polymers; C473966:poly(phenylene ethynylene)