title: Isolation and characterization of bluensomycin biosynthetic genes from Streptomyces bluensis.
abstract: The biosynthetic gene cluster for bluensomycin, a member of the aminoglycoside family of antibiotics, was isolated and characterized from the bluensomycin producing strain, Streptomyces bluensis ATCC27420. PCR primers were designed specifically to amplify a segment of the dTDP-glucose synthase gene based on its conserved sequences among several actinomycete strains. By screening a cosmid library using amplified PCR fragments, a 30-kb DNA fragment was isolated. Sequence analysis identified 15 open reading frames (ORFs), eight of which had previously been identified by Piepersberg et al. But seven are novel to this study. We demonstrated that one of these ORFs, blmA, confers resistance against the antibiotic dihydrostreptomycin, and another, blmD, encodes a dTDP-glucose synthase. These findings suggest that the isolated gene cluster is very likely to be responsible for the biosynthesis of bluensomycin.
authors.affiliation: Department of Biological Science, Institute of Bioscience and Biotechnology, Myong Ji University, 449-728, Yongin, South Korea/
authors.forename: Yong Gyun
authors.initials: YG
authors.lastname: Jung
authors.forename: Suk Ho
authors.initials: SH
authors.lastname: Kang
authors.forename: Chang Gu
authors.initials: CG
authors.lastname: Hyun
authors.forename: Young Yell
authors.initials: YY
authors.lastname: Yang
authors.forename: Choong Min
authors.initials: CM
authors.lastname: Kang
authors.forename: Joo Won
authors.initials: JW
authors.lastname: Suh
mesh_terms: D002240:Carbohydrate Sequence; D003001:Cloning, Molecular; D004096:Dihydrostreptomycin Sulfate / Q000031:analogs & derivatives* / Q000276:immunology / Q000378:metabolism*; D005798:Genes, Bacterial; D005947:Glucose / Q000031:analogs & derivatives* / Q000378:metabolism; D005958:Glucosephosphates / Q000378:metabolism; D008359:Mannose-6-Phosphate Isomerase / Q000032:analysis; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D009713:Nucleotidyltransferases / Q000032:analysis; D016366:Open Reading Frames; D013302:Streptomyces / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D013942:Thymine Nucleotides / Q000378:metabolism
chemical_list: D005958:Glucosephosphates; D013942:Thymine Nucleotides; C103522:deoxythymidine diphosphate-glucose; C031590:glucose-1-phosphate; D009713:Nucleotidyltransferases; C085243:dTDP-D-glucose synthase; D008359:Mannose-6-Phosphate Isomerase; D005947:Glucose; C006786:bluensomycin; D004096:Dihydrostreptomycin Sulfate