title: Effect of preservative, antioxidant and viscolizing agents on in vitro transcorneal permeation of ketorolac tromethamine.
abstract: The influence of formulation additives, e.g. preservative, antioxidant and viscolizing agents on in vitro transcorneal permeation of ketorolac tromethamine from 0.5%(w/v) aqueous drop was studied using goat cornea. Permeation characteristics of drug, from selected formulations, through excised rabbit cornea were also evaluated. Aqueous solution of ketorolac tromethamine (0.5% w/v), pH 6.5 or 7.0 having ionic strength 0.2, was prepared. To this solution perservatives either alone or in combination with other additives were added to have drops of various composition. Permeation studies with goat cornea showed maximum permeation of ketorolac tromethamine from formulation containing benzalkonium chloride and disodium edetate. Increase in viscosity of drop resulted in decreased permeation of drug. Formulation containing benzalkonium chloride and disodium edetate also increased permeation of drug through rabbit cornea. Cumulative permeation of drug through rabbit cornea was found to be 2.3-2.4 fold higher than that observed with goat cornea.
authors.affiliation: Department of Pharmaceutics, College of Pharmacy ( University of Delhi ), Pushp Vihar, Sector-III, New Delhi 110 017, India.
authors.forename: Manjusha
authors.initials: M
authors.lastname: Malhotra
authors.forename: D K
authors.initials: DK
authors.lastname: Majumdar
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000493:pharmacokinetics*; D000975:Antioxidants / Q000494:pharmacology*; D003315:Cornea / Q000378:metabolism*; D006041:Goats; D066298:In Vitro Techniques; D020911:Ketorolac Tromethamine / Q000493:pharmacokinetics*; D011817:Rabbits
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D020911:Ketorolac Tromethamine