Characterization of DeY1, a novel Y-box gene specifically expressed in differentiating male germ cells of planarians.
Y-box proteins are conserved regulatory factors that play a key role in coordinating gene activity with protein synthesis by influencing both the transcription and translation of specific subsets of genes. We report the identification of a novel Y-box gene, DeY1, whose transcripts are found in the testes of sexual planarians. DeY1 is expressed in spermatogonia, spermatocytes and spermatids, while no expression is detected in spermatozoa. No DeY1 transcripts are found in the blastema during regeneration. The subcellular distribution of DeY1 protein was analyzed by electron microscope immunocytochemistry. Immunolabelling was found in the nucleus of spermatogonia, in both the nucleus and the cytoplasm of spermatocytes, and in the cytoplasm of spermatids.
Salvetti
Alessandra
A
Dipartimento di Morfologia Umana e Biologia Applicata, Sezione di Biologia e Genetica, Università di Pisa, Pisa, Italy.
Lena
Annalisa
A
Rossi
Leonardo
L
Deri
Paolo
P
Cecchettini
Antonella
A
Batistoni
Renata
R
Gremigni
Vittorio
V
D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics; D029721:Drosophila Proteins; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010932:Planarians / Q000235:genetics* / Q000378:metabolism*; D014176:Protein Biosynthesis; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000378:metabolism / Q000648:ultrastructure
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C109692:YPS protein, Drosophila