title: Identification of hyaluronic acid oligosaccharides by direct coupling of capillary electrophoresis with electrospray ion trap mass spectrometry.
abstract: A new method for the identification of oligosaccharides obtained by enzymatic digestion of hyaluronic acid (HA) with bacterial hyaluronidase (HA lyase, E.C. 4.2.2.1, from Streptococcus agalactiae) using online capillary electrophoresis/electrospray mass spectrometry (CE/ESI-MS) is presented. A fused-silica capillary coated with polyacrylamide was used with a 40 mM ammonium acetate buffer at pH 9.0 and a separation voltage of +30 kV applied to the inlet. Separation was achieved for oligosaccharides containing 4-16 monomers. The migration behavior follows the chain length of the oligomers, regardless of charge state. However, no linear relationship was found for the relation between mobility and chain length. Using an ion trap mass analyzer, complementary structural information was obtained by MS/MS and MS(n) experiments.
authors.affiliation: Institute of Pharmaceutics and Biopharmaceutics, Martin-Luther-University Halle-Wittenberg, Wolfgang-Langenbeck-Str 4, 06120 Halle (S), Germany.
authors.forename: Andrea V
authors.initials: AV
authors.lastname: Kühn
authors.forename: Hans H
authors.initials: HH
authors.lastname: Rüttinger
authors.forename: Reinhard H H
authors.initials: RH
authors.lastname: Neubert
authors.forename: Klaus
authors.initials: K
authors.lastname: Raith
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D015394:Molecular Structure; D009844:Oligosaccharides / Q000032:analysis* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013292:Streptococcus agalactiae / Q000201:enzymology
chemical_list: D009844:Oligosaccharides; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase