Cell kinetics of human nasal septal chondrocytes in vitro: importance for cartilage grafting in otolaryngology.
In the field of reconstructive plastic surgery, grafts of autologous cartilage are sometimes used to replace damaged or pathologic tissues, particularly in the nose, ear, and trachea. However, this procedure is difficult to apply, especially because of the scarcity of donor sites. In this study, we have cultured and characterized human chondrocytes from human nasal septal cartilage biopsies. The proliferative activity was evaluated by incorporation of 3H-thymidine into deoxyribonucleic acid (DNA) both under normal culture conditions and with different growth factors and serum concentrations. Identification of chondrocytes in culture was performed with immunohistochemistry and production of matrix with specific histochemical indicators. We observed a significant increase of cell kinetics of differentiated chondrocytes, embedded with intense metachromatic matrix, in the presence of transforming growth factor beta, and low concentrations of fetal calf serum. Therefore, in suitable conditions, human chondrocytes obtained even from small specimens can produce in vitro considerable quantities of pure autologous cartilaginous tissue within a few days. This newly formed cartilage can be used as a grafting material in reconstructive surgery, particularly in otolaryngology.
Lavezzi
Anna
A
Institute of Pathology, University of Milan, Italy.
Mantovani
Mario
M
della Berta
Lorenzo Grillo
LG
Matturri
Luigi
L
D000328:Adult; D000818:Animals; D002356:Cartilage / Q000187:drug effects* / Q000254:growth & development* / Q000637:transplantation; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D002468:Cell Physiological Phenomena / Q000187:drug effects*; D019902:Chondrocytes / Q000187:drug effects* / Q000502:physiology*; D003470:Culture Media / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000187:drug effects / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008875:Middle Aged; D009300:Nasal Septum / Q000187:drug effects* / Q000254:growth & development*; D010038:Otorhinolaryngologic Diseases / Q000601:surgery*; D013517:Otorhinolaryngologic Surgical Procedures / Q000379:methods*; D016378:Tissue Transplantation / Q000379:methods*; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000494:pharmacology*
D003470:Culture Media; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2