title: In vitro culture and biochemical characterization of six trypanosome isolates from Peru and Brazil.
abstract: Six trypanosomatids isolated from different geographical areas from South America (Peru and Brazil) and different vectors and reservoir hosts (the triatomine Panstrongylus chinai [TP1], Triatoma infestans [TP2], Rhodnius ecuadorensis [TP3], R. prolixus [TB1], Didelphys marsupialis [TB2]), and one from a human asymptomatic patient [TB3], were characterized using lectin agglutination, isoenzyme profile, in vitro culture final metabolite patterns, and compared with a reference strain (Trypanosoma cruzi, Maracay strain [TC]). The different isolates were cultured in vitro in Grace's medium supplemented with 10% inactivated bovine foetal serum. According to our results and the statistical study, the isolate obtained from R. ecuadorensis should be designed as a Trypanosoma rangeli sp., showing all other isolates strong similarities to T. cruzi. Between them, two clusters could be identified, strongly correlating with the geographical origin. Cluster I grouped isolates from Peru and T. cruzi reference strain, and cluster II grouped the three Brazilian isolates.
authors.affiliation: Departamento de Parasitologia, Facultad de Ciencias, Universidad de Granada, C/Severo Ochoa s/n, 18071 Granada, Spain.
authors.forename: D M
authors.initials: DM
authors.lastname: Miralles
authors.forename: C
authors.initials: C
authors.lastname: Marín
authors.forename: R
authors.initials: R
authors.lastname: Magán
authors.forename: C
authors.initials: C
authors.lastname: Fernández-Ramos
authors.forename: E
authors.initials: E
authors.lastname: Entrala
authors.forename: O
authors.initials: O
authors.lastname: Cordova
authors.forename: F
authors.initials: F
authors.lastname: Vargas
authors.forename: M
authors.initials: M
authors.lastname: Sánchez-Moreno
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D001938:Brazil; D016000:Cluster Analysis; D004197:Disease Reservoirs; D006801:Humans; D007303:Insect Vectors; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D009893:Opossums; D010568:Peru; D014225:Triatominae; D014345:Trypanosoma / Q000737:chemistry / Q000145:classification / Q000201:enzymology / Q000254:growth & development*; D014349:Trypanosoma cruzi / Q000737:chemistry / Q000145:classification / Q000201:enzymology / Q000254:growth & development*
chemical_list: D007527:Isoenzymes