Potassium and sodium chloride ion pairs are presumed to constitute a complex during elution from a Sephadex G-15 column with sodium phosphate buffer.
When a mixed solution of 0.72 M potassium and sodium chloride was eluted from a Sephadex G-15 column with 0.025 M sodium phosphate buffer (pH 7.0), the elution profiles of ions showed that the potassium and chloride ion pair from the sample and the sodium and chloride ion pair produced by ion-exchange reaction, were eluted in the same fractions as if they constituted a complex. When a mixed solution of different concentrations of potassium and sodium chloride was eluted with the same buffer, the excess amount of one ion pair over the other was eluted freely from the presumed complex.
Okada
Toshihiko K
TK
Department of Biochemistry, Kanazawa Medical University, Daigaku 1-1, Uchinada, Ishikawa 920-0265, Japan. tk-okada@kanazawa-med.ac.jp
Nakabayashi
Y
Y
Miyakoshi
M
M
Inoue
M
M
D002021:Buffers; D003911:Dextrans / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry*; D011189:Potassium Chloride / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry*
D002021:Buffers; D003911:Dextrans; D010710:Phosphates; D012965:Sodium Chloride; D011189:Potassium Chloride; C025614:sephadex; C018279:sodium phosphate