cDNA microarray analysis of individual Duchenne muscular dystrophy patients.
We have developed a novel cDNA microarray encompassing 3500 genes expressed in skeletal muscle. With this system, we have performed the first study of gene expression in samples from individual patients. We analyzed muscle specimen from individuals with Duchenne muscular dystrophy to identify differences among patients. Among the variably expressed genes, we focused on the expression of the genes encoding HLA-related proteins, myosin light chains and troponin Ts as markers of muscle necrosis and regeneration. The expression patterns of these genes correlated with the severity of dystrophic changes on histological examination. Our cDNA microarray provides a new tool to investigate molecular muscle pathology.
Noguchi
Satoru
S
Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.
Tsukahara
Toshifumi
T
Fujita
Masako
M
Kurokawa
Rumi
R
Tachikawa
Masaji
M
Toda
Tatsushi
T
Tsujimoto
Atsumi
A
Arahata
Kiichi
K
Nishino
Ichizo
I
D002675:Child, Preschool; D018076:DNA, Complementary / Q000378:metabolism*; D015536:Down-Regulation; D015870:Gene Expression; D006801:Humans; D007223:Infant; D018482:Muscle, Skeletal / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D020388:Muscular Dystrophy, Duchenne / Q000235:genetics* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D020033:Protein Isoforms; D015854:Up-Regulation
D018076:DNA, Complementary; D020033:Protein Isoforms