title: Identification of mycobacterial species by PCR sequencing of quinolone resistance-determining regions of DNA gyrase genes.
abstract: The determination of the amino acid sequence of quinolone resistance-determining regions (QRDRs) in the A and B subunits of DNA gyrase is the molecular test for the detection of fluoroquinolone resistance in mycobacteria. We looked to see if the assignment of mycobacterial species could be obtained simultaneously by analysis of the corresponding nucleotide sequences. PCR sequencing of gyrA and gyrB QRDRs was performed for 133 reference and clinical strains of 21 mycobacterial species commonly isolated in clinical laboratories. Nucleotide sequences of gyrA and gyrB QRDRs were species specific, regardless of fluoroquinolone susceptibility.
authors.affiliation: Laboratoire de Bactériologie-Hygiène, Faculté de Médecine Pitié-Salpêtrière, Université Paris VI, France.
authors.forename: Jean-Noël
authors.initials: JN
authors.lastname: Dauendorffer
authors.forename: Isabelle
authors.initials: I
authors.lastname: Guillemin
authors.forename: Alexandra
authors.initials: A
authors.lastname: Aubry
authors.forename: Chantal
authors.initials: C
authors.lastname: Truffot-Pernot
authors.forename: Wladimir
authors.initials: W
authors.lastname: Sougakoff
authors.forename: Vincent
authors.initials: V
authors.lastname: Jarlier
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Cambau
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001483:Base Sequence; D027081:DNA Gyrase / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D024841:Fluoroquinolones; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D024841:Fluoroquinolones; D027081:DNA Gyrase