Reconstruction of deforming aortas in two-photon autofluorescence image sequences.
Information loss may occur frequently in the imaging of living tissues by using two-photon fluorescence microscopy due to the intensive deformation of the tissue. A landmark-based optical flow interpolation scheme is proposed for image reconstruction of living aorta walls in two-photon autofluorescence image sequences. Landmarks are extracted and evaluated by an active contour-based aorta model, and are aligned and reconstructed by use of a hierarchical algorithm. The accuracy of the calculation of optical flow is improved by applying landmark-based image warping. Experimental results show that the proposed scheme outperforms commonly used optical flow interpolation techniques for the reconstruction of intensively deforming tissues.
Wang
Jue
J
State Key Laboratory of Intelligent Technology and Systems, Department of Automation, Tsinghua University, Beijing 100084, China.
Ji
Liang
L
Ma
Hui
H
D001011:Aorta / Q000502:physiology*; D005453:Fluorescence; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D066298:In Vitro Techniques; D008856:Microscopy, Fluorescence; D008955:Models, Cardiovascular; D008962:Models, Theoretical; D017785:Photons