title: The relationship between RANTES and mast cells recruitment in the surroundings of intrahepatic implanted tumors.
abstract: OBJECTIVE
  To explore the relationship between the RANTES, TGFbeta1 and amount of mast cells (MC) surrounding the implanted tumors.
  
  
  METHODS
  Pieces of Walker 256 carcinosarcoma were implanted in the liver of 40 male Wistar rats and the formed intrahepatic implanted tumors were then divided into 3 groups: group without MC infiltration, group with little MC infiltration and group with MC infiltration; 8 normal rats served as control group. The sera of rats in the different groups were tested by ELISA to find the serum RANTES content of the tumor bearing rats, then the chemotactic activity of the serum RANTES of different tumor bearing groups vs peritoneal MC of normal rats was tested by the microBoyden chamber.
  
  
  RESULTS
  The MC amount of the tumor bearing rats was quite different, some of them showed a significantly increased amount. The groups with more MC infiltration showed a higher RANTES content in the sera and a stronger chemotactic activity vs MC.
  
  
  CONCLUSIONS
  The RANTES was an effective chemotactic factor to MC. The serum concentration of RANTES of the tumor bearing rats is related to the difference of the MC amount surrounding the tumor.
authors.affiliation: Department of Ultrastructural Pathology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Youbing
authors.initials: Y
authors.lastname: Ruan
authors.forename: Yang
authors.initials: Y
authors.lastname: Guan
authors.forename: Zhongbi
authors.initials: Z
authors.lastname: Wu
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Chunbing
authors.initials: C
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002279:Carcinoma 256, Walker / Q000097:blood / Q000473:pathology*; D018946:Chemokine CCL5 / Q000097:blood*; D002633:Chemotaxis; D008114:Liver Neoplasms, Experimental / Q000097:blood / Q000473:pathology*; D008297:Male; D008407:Mast Cells / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D016212:Transforming Growth Factor beta / Q000097:blood; D053773:Transforming Growth Factor beta1
chemical_list: D018946:Chemokine CCL5; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1