Distinguishing between different pathways of bilayer disruption by the related antimicrobial peptides cecropin B, B1 and B3.
Different pathways of bilayer disruption by the structurally related antimicrobial peptides cecropin B, B1 and B3, revealed by surface plasma resonance analysis of immobilized liposomes, differential scanning calorimetry of peptide-large unilamellar vesicle interactions, and light microscopic analysis of peptide-treated giant unilamellar vesicles, have been identified in this study. Natural cecropin B (CB) has one amphipathic and one hydrophobic alpha-helix, whereas cecropins B1 (CB1) and B3 (CB3), which are custom-designed, chimaeric analogues of CB, possess either two amphipathic or two hydrophobic alpha-helices, respectively. Surface plasma resonance analysis of unilamellar vesicles immobilized through a biotin-avidin interaction showed that both CB and CB1 bind to the lipid bilayers at high concentration (>10 microm); in contrast, CB3 induces disintegration of the vesicles at all concentrations tested. Differential scanning calorimetry showed the concentration-dependent effect of bilayer disruption, based on the different thermotrophic phase behaviours and the shapes of the thermal phase-transition curves obtained. The kinetics of the lysis of giant unilamellar vesicles observed by microscopy demonstrated that both CB and CB1 effect a continuous process involving loss of integrity followed by coalescence and resolution into smaller vesicles, whereas CB3 induces rapid formation of irregular-shaped, nonlamellar structures which rapidly disintegrate into twisted, microtubule-containing debris before being completely destroyed. On the basis of these observations, models by which CB, CB1 and CB3 induce lysis of lipid bilayers are discussed.
Chen
Hueih Min
HM
Institute of BioAgricultural Sciences, Academia Sinica, Taipei, Taiwan 115. robell@gate.sinica.edu.tw
Leung
King Wong
KW
Thakur
Nagendra N
NN
Tan
Anmin
A
Jack
Ralph W
RW
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002152:Calorimetry, Differential Scanning; D019476:Insect Proteins / Q000737:chemistry / Q000494:pharmacology*; D008051:Lipid Bilayers; D008969:Molecular Sequence Data; D010455:Peptides; D020033:Protein Isoforms / Q000737:chemistry / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D019476:Insect Proteins; D008051:Lipid Bilayers; D010455:Peptides; D020033:Protein Isoforms; C031162:cecropin B protein, Insecta