title: RBL cells expressing human Fc epsilon RI are a sensitive tool for exploring functional IgE-allergen interactions: studies with sera from peanut-sensitive patients.
abstract: Rat basophilic leukemia cells (RBL SX-38) express the alpha, beta, and gamma chains of human Fc epsilon RI. Following sensitization with IgE from a subset of allergic human donors, these cells can be triggered by exposure to anti-IgE or to very low concentrations of specific allergens. We examined 18 sera from patients who were highly sensitive to peanuts by history and had anti-peanut IgE by in vitro testing. The ability of these sera to sensitize the RBL SX-38 cells for degranulation with peanut allergens correlates very well with the absolute amount of anti-peanut IgE (r=0.95; p<0.001). The most effective sera contained at least 50 kU/l of total IgE and at least 15 kU/l of peanut-specific IgE. RBL SX-38 cells sensitized with these sera degranulated optimally upon exposure to anti-IgE (net degranulation of 40+/-8%, means+/-S.D.; n=8) and to a 10(5)-10(6) dilution of crude peanut extract (CPE) (37+/-7% net degranulation; 93+/-13% of that seen with anti-IgE). This assay is quite sensitive. Cells sensitized with selected sera are activated by exposure to a 1:10(7) dilution of the CPE containing picogram amounts of peanut allergens. This assay is also quite specific. Cells sensitized with sera from patients with anti-peanut IgE and no detectable IgE against soybean, walnut or grass pollen did not degranulate following exposure to these latter antigens. The converse was also true; cells sensitized with sera from patients without anti-peanut IgE did not react to peanut. These data demonstrate that RBL cells expressing human Fc epsilon RI form the basis of a useful model system for the detection of allergens and for the study of IgE-allergen interactions.
authors.affiliation: Division of Allergy and Clinical Immunology, University of Colorado Health Sciences Center, Medical School Building, Room 4627, 4200 East 9th Avenue, Denver, CO 80262, USA.
authors.forename: Donald A
authors.initials: DA
authors.lastname: Dibbern
authors.forename: G William
authors.initials: GW
authors.lastname: Palmer
authors.forename: P Brock
authors.initials: PB
authors.lastname: Williams
authors.forename: S Allan
authors.initials: SA
authors.lastname: Bock
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Dreskin
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology*; D010367:Arachis / Q000276:immunology*; D015550:Cell Degranulation; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D007158:Immunologic Techniques; D007223:Infant; D008407:Mast Cells / Q000276:immunology*; D008875:Middle Aged; D021183:Peanut Hypersensitivity / Q000276:immunology*; D017455:Receptors, IgE / Q000378:metabolism*; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured
chemical_list: D000485:Allergens; D017455:Receptors, IgE; D007073:Immunoglobulin E