Effect of heavy metals and PAH on soil assessed via dehydrogenase assay.
Dehydrogenase enzyme activity (DHA) assay method using resazurin was accommodated for measuring of toxicity of compound contaminants on uncharacterized microbial communities present in any given soil. The method was used to compare the toxic effect of heavy metal and polycyclic aromatic hydrocarbon (PAH) contaminant mixture (Cr, Pb, Cu, Cd, Pyrene) on four typical Estonian soils covering a range of compositions. The method proved to be useable on all soils; the sensitivity of soil microbiology to toxic effect of contaminants was found to have a negative correlation with Ca and organic matter (OM) content and a positive correlation with amorphous phase content of soils.
Irha
Natalya
N
National Institute of Chemical Physics and Biophysics, Akadeemia 23, 12618, Tallinn, Estonia. natalja@kbfi.ce
Slet
Jörgen
J
Petersell
Valter
V
D001681:Biological Assay / Q000379:methods; D004784:Environmental Monitoring; D019216:Metals, Heavy / Q000009:adverse effects* / Q000032:analysis; D009930:Organic Chemicals / Q000378:metabolism; D010088:Oxidoreductases / Q000032:analysis / Q000494:pharmacology*; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects* / Q000032:analysis; D012680:Sensitivity and Specificity; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000009:adverse effects* / Q000032:analysis
D019216:Metals, Heavy; D009930:Organic Chemicals; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; D010088:Oxidoreductases