title: Asynchronous differentiation models explain bone marrow labeling kinetics and predict reflux between the pre- and immature B cell pools.
abstract: B lymphopoiesis has historically been depicted as a unidirectional process, in which cohorts of developing cells transit through successive differentiative stages in an irreversible, synchronous manner. Here, we examine this view by combining kinetic analysis of developing B cell subsets in the bone marrow with mathematical modeling. Our bromo-deoxyuridine (BrdU) labeling data are incompatible with B cell development being a synchronous process, because labeling curves are non-linear. Moreover, we show that B cell development may not be completely unidirectional, because our results support the possibility of a phenotypic "reflux" among the immature to the pre-B cell subsets.
authors.affiliation: Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel. mehrra@mail.biu.ac.il
authors.forename: Ramit
authors.initials: R
authors.lastname: Mehr
authors.forename: Gitit
authors.initials: G
authors.lastname: Shahaf
authors.forename: Alex
authors.initials: A
authors.lastname: Sah
authors.forename: Michael
authors.initials: M
authors.lastname: Cancro
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D001853:Bone Marrow / Q000502:physiology*; D001973:Bromodeoxyuridine / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008954:Models, Biological; D013194:Staining and Labeling
chemical_list: D001973:Bromodeoxyuridine