Selenium supplementation can protect cultured rat cardiomyocytes from hypoxia/reoxygenation damage.
The possibility of enhancing glutathione peroxidase (GPx) activity and cytosolic total antioxidant activity (TAA) in normoxia and hypoxia/reoxygenation (H/R) by the supplementation of different concentrations of sodium selenite (SS) or selenomethionine (SM) was investigated in cultured rat cardiomyocytes. To assess the entity of oxidative stress due to H/R, levels of conjugated dienes containing lipids were determined. In normoxia, GPx activity and TAA increased in parallel with the increase in SS and SM supplementation. H/R did not influence GPx activity but lowered TAA; both SS and SM supplementations were effective in increasing GPx activity, the most effective concentration being 1 microM. At this SS and SM concentration, TAA returned to a normoxic value. Conjugated diene production, increased by H/R, was reduced by SS and SM supplementation, the 1 microM concentration appearing to be the most effective one. According to these data Se supplementation represents another possibility to counteract oxidative damage in the myocardium.
Bordoni
Alessandra
A
Nutrition Research Center, Department of Biochemistry G. Moruzzi, University of Bologna, via Irnerio 48, Italy. bordoni@biocfarm.unibo.it
Biagi
Pier L
PL
Angeloni
Cristina
C
Leoncini
Emanuela
E
Muccinelli
Ivan
I
Hrelia
Silvana
S
D000818:Animals; D000975:Antioxidants / Q000032:analysis; D015687:Cell Hypoxia / Q000187:drug effects*; D002478:Cells, Cultured; D005979:Glutathione Peroxidase / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D010100:Oxygen / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000008:administration & dosage*; D012645:Selenomethionine / Q000008:administration & dosage; D018038:Sodium Selenite / Q000008:administration & dosage
D000975:Antioxidants; D012645:Selenomethionine; D005979:Glutathione Peroxidase; D012643:Selenium; D018038:Sodium Selenite; D010100:Oxygen