[Variation of gene at the apolipoprotein C III locus with the changes of serum lipid profile in school-aged children].
The variation of gene at the apolipoprotein C III locus with the changes of serum lipids in children was studied. Blood samples were collected from 308 normal children aged 7-11 year-old, including of 151 boys and 157 girls. The levels of serum lipids profile, including TC, TG, LDL-C, HDL-C, apoB, apoA I and Lp(a) were detected. The genome DNA was extracted from blood clot, then ApoB-Xba I gene polymorphism were tested by the PCR-RFLP method. The frequency of ApoC III-Sac I heterozygote(+/-) was 48.7%, homozygote (+/+) was 7.5%, and allele (+) was 31.8% of the 308 children. The frequency of allele(+) was more than the reports in Shanghai (12%) and Guassian (6%), and closed to the Japanese (34%). The results showed a population and ethnic difference in the inheritance variation. Comparing the serum lipids levels among different genotypes, the average TG levels of homozygotes(+/+) were more than wildtype (-/+)(1.06 mmol/L vs 0.85 mmol/L, P < 0.05). The frequency of(+/+) genotype in hypertriglyceridemia group was more than control group (30.0% vs 6.7%, P < 0.05); The allele(+) could increase the TG level of 0.031 mmol/L. It suggested that the variation of Apolipoprotein C III-Sac I locus was correlated with the TG level in children.
Zhu
Wenli
W
Department of Nutrition and Food Hygiene, Health Science Center, Peking University, Beijing 100083, China.
Feng
Ningping
N
Wang
Ying
Y
D000483:Alleles; D053305:Apolipoprotein C-III; D001056:Apolipoproteins C / Q000235:genetics*; D002648:Child; D002784:Cholesterol / Q000097:blood; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D015228:Hypertriglyceridemia / Q000235:genetics; D008055:Lipids / Q000097:blood*; D008297:Male; D009154:Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014280:Triglycerides / Q000097:blood
D053305:Apolipoprotein C-III; D001056:Apolipoproteins C; D008055:Lipids; D014280:Triglycerides; D002784:Cholesterol