Efficient release of overproduced gene products from Escherichia coli BL21(DE3) by lytic infection with newly isolated bacteriophages.
Overproduced proteins from Escherichia coli BL21(DE3) were efficiently released with virulent bacteriophages. Leviviridae-like bacteriophages were isolated from soil and used to lyse BL21(DE3) cells transformed with beta-glucosidase, chitinase, or chitosanase genes. This method caused lysis of bacterial cells similar to that by conventional sonication and enabled us to effectively recover and purify the enzymes.
Iida
Yuichiro
Y
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University, Nara 631-8505, Japan.
Matsuda
Yoshinori
Y
Saito
Ryuichiro
R
Nakasato
Masanori
M
Nonomura
Teruo
T
Kakutani
Koji
K
Tosa
Yukio
Y
Mayama
Shigeyuki
S
Toyoda
Hideyoshi
H
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D002688:Chitinases / Q000096:biosynthesis / Q000302:isolation & purification; D003851:Deoxyribonucleases / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000302:isolation & purification; D017907:Leviviridae / Q000737:chemistry*; D008854:Microscopy, Electron; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000302:isolation & purification; D012260:Ribonucleases / Q000737:chemistry; D012988:Soil Microbiology; D014465:Ultrasonics; D001617:beta-Glucosidase / Q000096:biosynthesis / Q000302:isolation & purification
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D003851:Deoxyribonucleases; D012260:Ribonucleases; D006026:Glycoside Hydrolases; C019868:chitosanase; D002688:Chitinases; D001617:beta-Glucosidase