title: The making of "transgenic spermatozoa".
abstract: The processes of making transgenic animals by microinjecting DNA into the pronucleus of a fertilized oocyte or after the transfection of embryonic stem cells are now well established. However, attempts have also been made, with varying degrees of success, to use spermatozoa as a vector for transgenesis in mammals and other vertebrates during the last decade. A number of different approaches for making transgenic spermatozoa have been developed. These include directly incubating mature, isolated spermatozoa with DNA or pretreating mature, isolated spermatozoa before assisted fertilization. Microinjection procedures have also been established to transfect male germ cells directly in vivo within the seminiferous tubules or to reimplant previously isolated male germ cells submitted to in vitro transfection into a recipient testis. The latter two techniques present the advantage of being able to create transgenic progeny simply by mating with wild-type females, which avoids the possibility of interference or damage as a result of assisted fertilization or the manipulation of embryos. The different aspects of sperm-mediated transgenesis are presented.
authors.affiliation: Groupe d'Etude de la Reproduction chez le Mâle, INSERM U.435, Université de Rennes I, Campus de Beaulieu, 35042 Rennes Cedex, Bretagne, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Celebi
authors.forename: Thierry
authors.initials: T
authors.lastname: Guillaudeux
authors.forename: Pierrïck
authors.initials: P
authors.lastname: Auvray
authors.forename: Virginie
authors.initials: V
authors.lastname: Vallet-Erdtmann
authors.forename: Bernard
authors.initials: B
authors.lastname: Jégou
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D018014:Gene Transfer Techniques; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008845:Microinjections; D030781:Organisms, Genetically Modified / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D004247:DNA