HPLC for in-process control in the production of sultamicillin.
A high-performance liquid chromatographic assay coupled with UV detection (215 nm) was developed for the determination of sultamicillin and its synthesis precursors. The separation of the analytes was performed on a Kromasil C(18) column (15 cm x 4.6 mm i.d., 5 microm) at 20 degrees C. The mobile phase (25 mM phosphate buffer, pH 7.0 and acetonitrile 48%) was pumped at a flow rate of 1.0 ml min(-1). This method is sensitive (limits of detection ranged between 0.4 and 1.2 mg l(-1)) and selective for the determination of sultamicillin and could be used for monitoring different synthetic routes.
Laviana
L
L
MIT Fellowship at Asturpharma S.A., Silvota, Llanera, Asturias, Spain.
Fernández-Marí
F
F
Bayod
M
M
Blanco
D
D
D000667:Ampicillin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004359:Drug Therapy, Combination / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013407:Sulbactam / Q000032:analysis*
C035444:sultamicillin; D000667:Ampicillin; D013407:Sulbactam