title: Hepatic lipase mediates an increase in selective uptake of HDL-associated cholesteryl esters by cells in culture independent from SR-BI.
abstract: Scavenger receptor class B type I (SR-BI) mediates the selective uptake of HDL cholesteryl esters (CEs) by the liver. Hepatic lipase (HL) promotes this lipid uptake independent from lipolysis. The role of SR-BI in this HL-mediated increase in selective CE uptake was explored. Baby hamster kidney (BHK) cells were transfected with the SR-BI cDNA yielding cells with SR-BI expression, whereas no SR-BI was detected in control cells. These cells were incubated in medium containing 125I [3H]cholesteryl oleyl ether-labeled HDL3 (d = 1.125-1.21 g/ml) and HL was absent or present. Tetrahydrolipstatin (THL) blocked lipolysis. In control BHK cells and in BHK cells with SR-BI, HDL3 selective CE uptake (3H-125I) was detectable and SR-BI promoted this uptake. In both cell types, HL mediated an increase in selective CE uptake from HDL3. Quantitatively, this HL effect was similar in control BHK cells and in BHK cells with SR-BI. These results suggest that HL promotes selective uptake independent from SR-BI. To investigate the role of cell surface proteoglycans on the HL-mediated HDL3 uptake, proteoglycan deficiency was induced by heparinase digestion. Proteoglycan deficiency decreased the HL-mediated promotion of selective CE uptake. In summary, the stimulating HL effect on HDL selective CE uptake is independent from SR-BI and lipolysis. Proteoglycans are a requisite for the HL action on selective uptake. Results suggest that (a) pathway(s) distinct from SR-BI mediate(s) selective CE uptake from HDL.
authors.affiliation: Universitaetsklinikum Hamburg-Eppendorf, Department for Internal Medicine, Martinistrasse 52, 20246 Hamburg, Germany.
authors.forename: May
authors.initials: M
authors.lastname: Brundert
authors.forename: Joerg
authors.initials: J
authors.lastname: Heeren
authors.forename: Heiner
authors.initials: H
authors.lastname: Greten
authors.forename: Franz
authors.initials: F
authors.lastname: Rinninger
mesh_terms: D000818:Animals; D016632:Apolipoprotein A-I / Q000378:metabolism; D018955:CD36 Antigens / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D002788:Cholesterol Esters / Q000378:metabolism / Q000493:pharmacokinetics*; D008076:Cholesterol, HDL / Q000378:metabolism / Q000493:pharmacokinetics*; D003001:Cloning, Molecular; D006224:Cricetinae; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015151:Immunoblotting; D007783:Lactones / Q000494:pharmacology; D008049:Lipase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008565:Membrane Proteins; D000077403:Orlistat; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D013997:Time Factors; D014162:Transfection
chemical_list: D016632:Apolipoprotein A-I; D018955:CD36 Antigens; D002788:Cholesterol Esters; D008076:Cholesterol, HDL; D004791:Enzyme Inhibitors; D007783:Lactones; D008565:Membrane Proteins; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C495124:SCARB1 protein, human; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; D000077403:Orlistat; D008049:Lipase