title: [Antimicrobial susceptibility and beta-lactamase productivity of recent clinical isolates during the period between December 1999 and February 2000].
abstract: Antimicrobial susceptibility testings of 24 antimicrobial agents against 605 clinical strains belonging to 10 species were carried out according to the micro-broth dilution method of NCCLS M7-A4. The productivity of beta-lactamase was also determined against them isolated at 8 medical facilities in Nagano prefecture, Japan during the period between December 1999 and February 2000. When applied the nitrocefin method, beta-lactamase productivity was demonstrated to be positive for 89.2% of 74 S. aureus, 4.3% of 94 H. influenzae, and 100% of 69 M. (B.) catarrhalis isolates. On the other hand, when used the acidometry method, penicillinase/cephalosporinase were found to be positive for 21.2%/9.6% of 52 E. coli, 29.0%/3.2% of 31 K. pneumoniae, 53.2%/100% of 47 E. cloacae, 0%/11.1% of 99 S. marcescens, and 25.9%/55.6% of 54 P. aeruginosa isolates, respectively. Among the beta-lactamase-producers including P. aeruginosa isolates, only 2 E. coli isolates were found to be ESBL-producers. Besides, 9.6% (9/94) of H. influenzae isolates were proved to be BLNAR strains.
authors.affiliation: Miroku Medical Laboratory.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Notake
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Hideji
authors.initials: H
authors.lastname: Yanagisawa
authors.forename: Makio
authors.initials: M
authors.lastname: Haketa
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ide
authors.forename: Keiko
authors.initials: K
authors.lastname: Nagai
authors.forename: Jiro
authors.initials: J
authors.lastname: Kakegawa
authors.forename: Junko
authors.initials: J
authors.lastname: Shiozawa
authors.forename: Satoru
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Shimazaki
authors.forename: Miharu
authors.initials: M
authors.lastname: Naitoh
authors.forename: Kenichi
authors.initials: K
authors.lastname: Toda
authors.forename: Kyoko
authors.initials: K
authors.lastname: Fujiki
authors.forename: Yukie
authors.initials: Y
authors.lastname: Okimura
authors.forename: Tadashi
authors.initials: T
authors.lastname: Okabe
mesh_terms: D001419:Bacteria / Q000187:drug effects* / Q000201:enzymology*; D024881:Drug Resistance, Bacterial; D016972:Enterobacter cloacae / Q000187:drug effects / Q000201:enzymology; D013293:Enterococcus faecalis / Q000187:drug effects / Q000201:enzymology; D016984:Enterococcus faecium / Q000187:drug effects / Q000201:enzymology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D006193:Haemophilus influenzae / Q000187:drug effects / Q000201:enzymology; D007564:Japan; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000254:growth & development; D001936:Moraxella catarrhalis / Q000187:drug effects / Q000201:enzymology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology; D012706:Serratia marcescens / Q000187:drug effects / Q000201:enzymology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D001618:beta-Lactamases