Genotypic and phenotypic characteristics of antibiotic-producing soil Streptomyces investigated by RAPD-PCR.
Random amplified polymorphic DNA (RAPD) analysis has been used to determine the relatedness of 73 antibiotic-producing soil Streptomyces isolates that were recovered from different soil habitats in Jordan based on their RAPD-PCR fingerprints. Genetic polymorphisms between these isolates showed three common bands of 2777, 800 and 250 bp shared by approximately (95%) of them. Some specific bands were also observed. Further analysis of RAPD patterns with the UPGMA resulted in clustering the tested isolates into two main super clusters. Super cluster I was more homogenous than super cluster II and contained all the reference strains. However, super cluster II consists of unrelated isolates within five small groups. As RAPD fingerprints of the tested isolates linked to their phenotypes, differentiation between isolates with different cultural properties was observed.
Gharaibeh
Raad
R
Department of Applied Biological Sciences, Jordan University of Science and Technology, Irbid-22110, Jordan.
Saadoun
Ismail
I
Mahasneh
Amjad
A
D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D016000:Cluster Analysis; D003116:Color; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005838:Genotype; D010641:Phenotype; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial