Plasma protein adsorption patterns on surfaces of Amphotericin B-containing fat emulsions.
Nephrotoxicity of the conventional Amphotericin B formulation Fungizone is the most common side effect in treatment of systemic fungal infections. Lipid formulations of Amphotericin B including fat emulsions showed a reduced nephrotoxicity. In vivo distribution studies of lipid formulations have shown an accumulation of Amphotericin B in liver and spleen, while concentration in the kidneys is reduced. Blood proteins adsorbed onto particles after intravenous administration are regarded as the key factors determining their in vivo fate. Two-dimensional polyacrylamid gel electrophoresis is a powerful tool for analysis of protein adsorption patterns. This paper deals with the question if there is any correlation between proteins adsorbed on surfaces of AmB fat emulsions produced with a new production technique and the potentially organ distribution of this formulation.
Schmidt
Sven
S
Pharmazeutische Technologie, Biopharmazie und Biotechnologie, Institut für Pharmazie, Freie Universität Berlin, Kelchstrasse 31, D-12169 Berlin, Germany.
Müller
Rainer H
RH
D000327:Adsorption; D000666:Amphotericin B / Q000737:chemistry* / Q000493:pharmacokinetics; D000935:Antifungal Agents / Q000737:chemistry* / Q000493:pharmacokinetics; D001798:Blood Proteins / Q000378:metabolism*; D002626:Chemistry, Pharmaceutical; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005217:Fat Emulsions, Intravenous / Q000737:chemistry; D066298:In Vitro Techniques; D008081:Liposomes; D016622:Silver Staining; D014018:Tissue Distribution
D000935:Antifungal Agents; D001798:Blood Proteins; D005217:Fat Emulsions, Intravenous; D008081:Liposomes; D000666:Amphotericin B