Effect of Pluronic-block copolymers on the reduction of serum-mediated inhibition of gene transfer of polyethyleneimine-DNA complexes.
Serum stability of non-viral vectors is a crucial factor for successful in vivo gene delivery. Pluronic-block copolymers consisting of hydrophilic ethylene oxide and hydrophobic propylene oxide blocks were tested to prevent the reduction of serum-mediated inhibition of gene transfer of polyethyleneimine (PEI)-DNA complexes in NIH/3T3 cells. The order of hydrophilic-lipophilic balance (HLB) of six different types of Pluronics used in this study was F68>F127>P105>P94>L122>L61. Transfection activities of NIH/3T3 cells with PEI-DNA complexes containing Pluronics with higher HLB showed marked improvement of gene-expression levels in serum media from 10 to 50% fetal bovine serum compared with PEI-DNA complexes alone. Also, higher concentrations (1 and 3%) of Pluronics with higher HLB in the PEI/DNA dispersion provided a stronger steric hindrance in resisting serum components than those obtained in a lower concentration (0.1%). These results suggested that non-viral vectors incorporated with higher HLB of Pluronics may be used as potential vehicles for in vivo delivery of DNA.
Kuo
Jung-Hua Steven
JH
Department of Pharmacy, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Rd, Sec. 1, Jen-Te, Tainan 717, Taiwan, Republic of China. kuojunghua@yahoo.com
D016475:3T3 Cells; D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000493:pharmacokinetics*; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics; D018014:Gene Transfer Techniques; D051379:Mice; D010957:Plasmids / Q000737:chemistry / Q000493:pharmacokinetics*; D011060:Poloxalene / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry / Q000493:pharmacokinetics*; D044967:Serum / Q000737:chemistry / Q000378:metabolism*; D014162:Transfection / Q000379:methods*
D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; C464484:pluronic block copolymer P123; D011094:Polyethyleneimine; D011060:Poloxalene