Dendriplexes and their characterisation.
The interaction of DNA with partial dendrimers (dendritic polylysine containing seven lysines and eight terminal amino groups with or without a lipidic core) was studied. Compact complexes were formed which we term "dendriplexes". Agarose gel electrophoresis and exclusion of ethidium bromide confirmed the interaction. All the dendrons formed compact complexes above a 2:1 (+/-) charge ratio in water and HBSS. Photon correlation spectroscopy, electron microscopy and zeta potential measurements were used to determine, respectively, the particle size, shape and surface charge of the dendriplexes. The z-average diameter of the dendriplexes were found to be 60-70 nm irrespective of the dendron used and the zeta potential varied from 10 to 35 mV at a 3:1 (+/-) charge ratio depending on the dendron. The protection of the DNA component of these dendriplexes from nuclease degradation was confirmed by DNase protection assays.
Ramaswamy
Chandrasekaran
C
Centre for Drug Delivery Research, The School of Pharmacy, University of London, 29/39 Brunswick Square, London WC1N 1AX, UK.
Sakthivel
Thiagarajan
T
Wilderspin
Andy F
AF
Florence
Alexander T
AT
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000737:chemistry*; D004337:Drug Carriers / Q000378:metabolism; D016503:Drug Delivery Systems; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004996:Ethidium / Q000378:metabolism; D050356:Lipid Metabolism; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods; D010316:Particle Size; D011107:Polylysine / Q000031:analogs & derivatives / Q000737:chemistry*
D004337:Drug Carriers; D011107:Polylysine; D004247:DNA; D004996:Ethidium