title: Crystallization and preliminary crystallographic studies of mung bean cytokinin-specific binding protein.
abstract: Cytokinins, or plant growth hormones, bind with very high affinity to cytokinin-specific binding proteins (CSBPs). Recombinant mung bean CSBP has been overexpressed in Escherichia coli and crystallized in complex with zeatin, a natural plant growth hormone. The crystals belong to the hexagonal system, space group P6(2) or P6(4), with unit-cell parameters a = 113.62, c = 86.85 A, contain two to five copies of the protein in the asymmetric unit and diffract X-rays to 1.25 A resolution.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland, and Institute of Technical Biochemistry, Technical University of Łódź, Poland.
authors.forename: Grzegorz D
authors.initials: GD
authors.lastname: Bujacz
authors.forename: Oliwia
authors.initials: O
authors.lastname: Pasternak
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Michal M
authors.initials: MM
authors.lastname: Sikorski
authors.forename: Mariusz
authors.initials: M
authors.lastname: Jaskolski
mesh_terms: D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry*; D011994:Recombinant Proteins; D015026:Zeatin / Q000737:chemistry
chemical_list: D002352:Carrier Proteins; D011994:Recombinant Proteins; D015026:Zeatin