Effectiveness of peptone-yeast extract (P-Y) medium in the cultivation and isolation of Entamoeba histolytica/Entamoeba dispar in Turkish patients.
Amebiasis is a common protozoan infection worldwide, causing serious health problems in both children and adults. Today, almost 10% of the world population is infected with Entamoeba histolytica/Entamoeba dispar. The aims of this study were both the comparison of the reproduction rates and densities of E. histolytica/E. dispar in Robinson, Dobell-Laidlaw and P-Y culture media and isolation of E. histolytica/E. dispar from stool samples in Peptone-Yeast (P-Y) medium. Trophozoites and cysts of E. histolytica/E. dispar, maintained in Robinson medium, and stool samples of patients with amebiasis were inoculated into P-Y, Robinson and Dobell-Laidlaw culture media. Reproduction rates reached their peak levels 48 h after the inoculation in all culture media. Reproduction rates in P-Y and Robinson media were found similar; however, they were higher than the reproduction rate in Dobell-Laidlaw medium (p < 0.01); there was no statistically significant difference between the reproduction rates of P-Y and Robinson media (p > 0.05). Twelve isolates from 12 patients were cultivated in P-Y medium and checked for reproduction everyday for 7 days. Twelve of the 12 (100%) isolates were cultivated in P-Y medium, indicating that the P-Y was an effective medium for the isolation of E. histolytica/E. dispar in stool samples. According to these results, P-Y medium could be preferred in immunologic, serologic and molecular studies and, thus the definitive diagnosis of amebiasis due to its low cost and simple formula.
Dagci
Hande
H
Department of Parasitology, Ege University School of Medicine, Izmir, Izmir, Turkey. hdagci@med.ege.edu.tr
Balcioglu
I Cuneyt
IC
Ertabaklar
Hatice
H
Kurt
Ozgur
O
Atambay
Metin
M
D000818:Animals; D002457:Cell Extracts; D002478:Cells, Cultured; D003470:Culture Media; D004747:Entamoeba / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification*; D004748:Entamoeba histolytica / Q000254:growth & development / Q000302:isolation & purification; D004749:Entamoebiasis / Q000382:microbiology*; D006801:Humans; D010461:Peptones / Q000737:chemistry*; D013997:Time Factors; D014421:Turkey; D015003:Yeasts / Q000166:cytology
D002457:Cell Extracts; D003470:Culture Media; D010461:Peptones