Comparative effects of basophil-directed growth factors.
IL-3, IL-5, and GM-CSF exert various overlapping functions in basophils. We investigated the receptor expression profiles and concentration-dependent effects of IL-3, IL-5, and GM-CSF on several basophil functions in comparison with their effects on eosinophils. The order of the receptor expression levels was IL-3Ralpha>IL-5Ralpha>GM-CSFRalpha in basophils and IL-5Ralpha>or=GM-CSFRalpha>IL-3Ralpha in eosinophils. Compared with eosinophils, basophils expressed a much higher level of IL-3Ralpha and similar levels of IL-5Ralpha and GM-CSFRalpha. The order of potency was IL-3>IL-5=GM-CSF for degranulation, survival, and CD11b expression in basophils, and IL-5=GM-CSF>or=IL-3 for survival and CD11b expression in eosinophils. However, IL-3 induced CD69 expression preferentially in basophils. Our results indicate that IL-3 is the most potent activator of human basophils, and that the rank order of potency of hemopoietic growth factors virtually corresponded to their receptor expression levels in both cell types.
Yoshimura-Uchiyama
Chitose
C
Department of Allergy and Rheumatology, University of Tokyo, Graduate School of Medicine, Japan.
Yamaguchi
Masao
M
Nagase
Hiroyuki
H
Fujisawa
Takao
T
Ra
Chisei
C
Matsushima
Kouji
K
Iwata
Tsutomu
T
Igarashi
Takashi
T
Yamamoto
Kazuhiko
K
Hirai
Koichi
K
D015703:Antigens, CD / Q000096:biosynthesis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis; D001491:Basophils / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D039481:CD11b Antigen / Q000096:biosynthesis; D015550:Cell Degranulation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004804:Eosinophils / Q000187:drug effects / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D016023:Integrins / Q000096:biosynthesis; D007377:Interleukin-3 / Q000494:pharmacology*; D053650:Interleukin-3 Receptor alpha Subunit; D015848:Interleukin-5 / Q000494:pharmacology*; D037181:Lectins, C-Type; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D016185:Receptors, Interleukin-3 / Q000096:biosynthesis; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D039481:CD11b Antigen; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D016023:Integrins; D007377:Interleukin-3; D053650:Interleukin-3 Receptor alpha Subunit; D015848:Interleukin-5; D037181:Lectins, C-Type; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D016185:Receptors, Interleukin-3; D000099192:CD69 Antigens; C115022:IL3RA protein, human