Changes in hydrogen peroxide homeostasis trigger an active cell death process in tobacco.
In transgenic tobacco plants with reduced catalase activity, high levels of hydrogen peroxide (H2O2) can accumulate under photorespiratory conditions. Such a perturbation in H2O2 homeostasis induced cell death in clusters of palisade parenchyma cells, primarily along the veins. Ultrastructural alterations, such as chromatin condensation and disruption of mitochondrial integrity, took place before cell death. Furthermore, enhanced transcript levels of mitochondrial defense genes accompanied these mitochondrial changes. Pharmacological data indicated that the initiation and execution of cell death require de novo protein synthesis and that the signal transduction pathway leading to cell death involved changes in ion homeostasis, (de)phosphorylation events and an oxidative burst, as observed during hypersensitive responses. This oxidase-dependent oxidative burst is essential for cell death, but it is not required for the accumulation of defense proteins, suggesting a more prominent role for the oxidative burst in abiotic stress-induced cell death.
Dat
James F
JF
Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Pellinen
Riikka
R
Beeckman
Tom
T
Van De Cotte
Brigitte
B
Langebartels
Christian
C
Kangasjärvi
Jaakko
J
Inzé
Dirk
D
Van Breusegem
Frank
F
D017209:Apoptosis / Q000502:physiology*; D002374:Catalase / Q000378:metabolism; D019069:Cell Respiration / Q000502:physiology; D006706:Homeostasis / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008027:Light; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D018515:Plant Leaves / Q000502:physiology; D030821:Plants, Genetically Modified; D015398:Signal Transduction / Q000502:physiology; D014026:Nicotiana / Q000235:genetics / Q000502:physiology*
D006861:Hydrogen Peroxide; D002374:Catalase