Molecular identification and antibody testing of Chlamydophila pneumoniae in a subgroup of patients with HIV-associated dementia complex. Preliminary results.
Chlamydophila pneumoniae DNA was investigated by polymerase chain reaction (PCR), in cerebrospinal fluid (CSF) specimens from patients suffering from HIV-1-associated dementia complex (HADC). Four (17.3%) cases of C. pneumoniae infection were identified among 23 HADC individuals with DNA amplification of major outer membrane protein (MOMP) gene and 16S rRNA gene sequences. Sequence analysis revealed significant homologies with C. pneumoniae compared to Chlamydia trachomatis and Chlamydia psittaci. High mean levels of CSF specific anti-C. pneumoniae antibodies and C. pneumoniae antibody specific index values significantly elevated were also found by enzyme-linked immunosorbent assay (ELISA) in these patients. The results suggest a hypothetical role of C. pneumoniae in the pathogenesis or progression of HADC.
Contini
Carlo
C
Section of Infectious Diseases, Department of Clinical and Experimental Medicine, via Fossato di Mortara, 23, University of Ferrara, Italy. cnc@dns.unife.it
Fainardi
Enrico
E
Seraceni
Silva
S
Granieri
Enrico
E
Castellazzi
Massimiliano
M
Cultrera
Rosario
R
D015526:AIDS Dementia Complex / Q000097:blood / Q000134:cerebrospinal fluid / Q000382:microbiology*; D000906:Antibodies / Q000134:cerebrospinal fluid; D000942:Antigens, Bacterial / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D002490:Central Nervous System / Q000276:immunology / Q000382:microbiology / Q000821:virology; D002690:Chlamydia Infections / Q000097:blood / Q000134:cerebrospinal fluid / Q000150:complications*; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity*; D004269:DNA, Bacterial / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D000906:Antibodies; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D008565:Membrane Proteins; D012313:RNA