In vitro interaction of macrocyclic photosensitizers with intact mitochondria: a spectroscopic study.
Six water-soluble macrocyclic photosensitizers, the members of two groups of expanded porphyrins (metallotexaphyrins and free-base sapphyrins) containing hydrophilic substituents and meso-tetra(4-sulfonatophenyl)-porphyrin, were tested by UV-Vis absorption and resonance Raman spectroscopy in the in vitro binding experiments with intact mitochondria isolated from swine liver. Studied macrocycles showed markedly different affinity to mitochondria. The highest uptake was observed for sapphyrin-sugar conjugate and metallotexaphyrins. Sapphyrin-polyamine conjugates exhibit something less affinity to mitochondria, while the porphyrin of anionic character showed very low mitochondrial uptake. Obtained spectroscopic results confirm that the binding process altered the self-aggregation degree of expanded porphyrins.
Synytsya
Alla
A
Faculty of Chemical Technology, Department of Analytical Chemistry, Institute of Chemical Technology, Technická 5, 166 28 Prague 6, Czech Republic. Alla.Synytsya@vscht.cz
Kral
Vladimir
V
Synytsya
Andriy
A
Volka
Karel
K
Sessler
Jonathan L
JL
D000465:Algorithms; D000818:Animals; D008930:Mitochondria, Liver / Q000737:chemistry*; D015394:Molecular Structure; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000138:chemical synthesis / Q000737:chemistry*; D011166:Porphyrins / Q000138:chemical synthesis / Q000737:chemistry*; D013053:Spectrophotometry; D013059:Spectrum Analysis, Raman; D013552:Swine
D017319:Photosensitizing Agents; D011166:Porphyrins