title: [Evaluation of eight diagnostic tests for HIV infection in Lome (Togo)].
abstract: This study to evaluate the performance of eight diagnostic tests for HIV/AIDS infection was conducted at the National Reference Center for HIV/AIDS/STD in Lomé, Togo. The tests were as follows: Enzymum test anti HIV Combi, Enzymum tests anti-HIV1 + 2 + subtype O, Genscreen HIV 1/2, Ice 1.0.2, Vironostika HIV Uni-Form II Plus O, Genie II HIV 1/2, SFD HIV 1/2 PA and DETERMINE HIV 1/2. A total of 238 serum specimens collected consecutively between January and April 1999 were studied. They were from 161 occasional blood donors and 77 patients. New Lav-Blot I and Ii (western blot) were used as reference tests. Test sensitivity ranged from 90 to 100%. Specificity ranged from 96 to 100%. The Enzymum test anti HIV Combi used only on serum samples from blood donors demonstrated a sensitivity and specificity of 100%. Tests based on Elisa (Emzymum Combi, Enzymum HIV 1 + 2 + subtype O, Genscreen, Ice 1.0.2 and Vironostika) allowed acceptable diagnosis of HIV/AIDS as alternatives to western blot. Two of the three rapid assays tested provided acceptable results, i.e., Genie II HIV 1/2 and SFD HIV 1/2. They are suitable for screening to prevent HIV transmission by blood transfusion in areas where Elisa is unfeasible.
authors.affiliation: Département de bactériologie-virologie, Centre Hospitalier et Universitaire Tokoin de Lomé, Togo. yaotse@yahoo.fr
authors.forename: A Y
authors.initials: AY
authors.lastname: Dagnra
authors.forename: M
authors.initials: M
authors.lastname: Prince David
authors.forename: J
authors.initials: J
authors.lastname: Gaba
authors.forename: M T
authors.initials: MT
authors.lastname: Ouro-Akpo
authors.forename: A Y
authors.initials: AY
authors.lastname: Segbena
authors.forename: K
authors.initials: K
authors.lastname: Ali-Edje
authors.forename: A
authors.initials: A
authors.lastname: Ehlan
authors.forename: F
authors.initials: F
authors.lastname: Bougoudogo
mesh_terms: D001803:Blood Transfusion; D015153:Blotting, Western; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D015658:HIV Infections / Q000175:diagnosis*; D006801:Humans; D011786:Quality Control; D012680:Sensitivity and Specificity; D014037:Togo
chemical_list: D004279:DNA, Viral