Identification of an indispensable amino acid for ppGpp synthesis of Escherichia coli SpoT protein.
Amino acid substitutions were introduced into a structurally flexible and highly conserved region of Escherichia coli SpoT protein. SpoT protein changed from Asp to Ala at the 293rd position did not restore cell growth of E. coli CF8295 (delta relA, delta spoT) and did not accumulate ppGpp in the cell, suggesting that the Asp293 is indispensable for ppGpp synthesis of the protein.
Fujita
Chizuko
C
Department of Chemistry, Faculty of Science, Nara Women's University, Kita-uoya-nishi-machi, Nara 630-8506, Japan.
Maeda
Maki
M
Fujii
Takako
T
Iwamoto
Ryoko
R
Ikehara
Kenji
K
D000409:Alanine / Q000235:genetics; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001224:Aspartic Acid / Q000235:genetics; D002455:Cell Division; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D006159:Guanosine Tetraphosphate / Q000096:biosynthesis / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D011755:Pyrophosphatases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D001224:Aspartic Acid; D006159:Guanosine Tetraphosphate; C027627:guanosine-3',5'-bis(diphosphate) 3'-pyrophosphatase; D011755:Pyrophosphatases; D000409:Alanine