The relationship between RANTES and mast cells recruitment in the surroundings of intrahepatic implanted tumors.
OBJECTIVE
To explore the relationship between the RANTES, TGFbeta1 and amount of mast cells (MC) surrounding the implanted tumors.


METHODS
Pieces of Walker 256 carcinosarcoma were implanted in the liver of 40 male Wistar rats and the formed intrahepatic implanted tumors were then divided into 3 groups: group without MC infiltration, group with little MC infiltration and group with MC infiltration; 8 normal rats served as control group. The sera of rats in the different groups were tested by ELISA to find the serum RANTES content of the tumor bearing rats, then the chemotactic activity of the serum RANTES of different tumor bearing groups vs peritoneal MC of normal rats was tested by the microBoyden chamber.


RESULTS
The MC amount of the tumor bearing rats was quite different, some of them showed a significantly increased amount. The groups with more MC infiltration showed a higher RANTES content in the sera and a stronger chemotactic activity vs MC.


CONCLUSIONS
The RANTES was an effective chemotactic factor to MC. The serum concentration of RANTES of the tumor bearing rats is related to the difference of the MC amount surrounding the tumor.
Ruan
Youbing
Y
Department of Ultrastructural Pathology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Guan
Yang
Y
Wu
Zhongbi
Z
Zhang
Zhiyong
Z
Zheng
Chunbing
C
D000818:Animals; D002279:Carcinoma 256, Walker / Q000097:blood / Q000473:pathology*; D018946:Chemokine CCL5 / Q000097:blood*; D002633:Chemotaxis; D008114:Liver Neoplasms, Experimental / Q000097:blood / Q000473:pathology*; D008297:Male; D008407:Mast Cells / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D016212:Transforming Growth Factor beta / Q000097:blood; D053773:Transforming Growth Factor beta1
D018946:Chemokine CCL5; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1