Modification by fluoride, bromide, iodide, thiocyanate and nitrite anions of reaction of a myeloperoxidase-H2O2-Cl- system with nucleosides.
The influence of fluoride (F(-)), bromide (Br(-)), iodide (I(-)), thiocyanate (SCN(-)) and nitrite (NO(2)(-)) on the reaction of a myeloperoxidase-H(2)O(2)-Cl(-) system with a nucleoside mixture was studied. The reaction was carried out under mildly acidic conditions and terminated by N-acetylcysteine. Without the additional anions, quantity of nucleosides consumed fell in the following order: 2'-deoxyguanosine>2'-deoxycytidine>>2'-deoxythymidine>2'-deoxyadenosine asymptotically equal to 0. F(-) did not affect the reaction. Br(-) increased the consumption of 2'-deoxycytidine and 2'-deoxythymidine, but decreased that of 2'-deoxyguanosine. I(-), SCN(-) and NO(2)(-) suppressed the reaction. These results suggest that Br(-) has a unique effect in relation to nucleoside damage caused by myeloperoxidase.
Suzuki
Toshinori
T
International Agency for Research on Cancer, Lyon, France.
Ohshima
Hiroshi
H
D000838:Anions / Q000032:analysis / Q000378:metabolism*; D001965:Bromides / Q000032:analysis / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005459:Fluorides / Q000032:analysis / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism*; D007454:Iodides / Q000032:analysis / Q000378:metabolism; D009573:Nitrites / Q000032:analysis / Q000378:metabolism; D009705:Nucleosides / Q000032:analysis / Q000378:metabolism*; D009195:Peroxidase / Q000032:analysis / Q000378:metabolism*; D013861:Thiocyanates / Q000032:analysis / Q000378:metabolism
D000838:Anions; D001965:Bromides; D007454:Iodides; D009573:Nitrites; D009705:Nucleosides; D013861:Thiocyanates; D006861:Hydrogen Peroxide; D009195:Peroxidase; C031760:thiocyanate; D005459:Fluorides