Substrate inhibition of L-cysteine alpha,beta-elimination reaction catalyzed by L-cystathionine gamma-lyase of Saccharomyces cerevisiae.
The alpha,beta-elimination of L-cysteine catalyzed by Saccharomyces cerevisiae L-cystathionine gamma-lyase (EC 4.4.1.1) was inhibited by the substrate. The absorption spectrum of the holoenzyme in the presence of L-cysteine showed that the substrate inhibition observed in this reaction was due mainly to removal of the cofactor.
Yamagata
Shuzo
S
Department of Biotechnology, Faculty of Agriculture, Gifu University, Gifu 501-1193, Japan. yamagata@cc.gifu-u.ac.jp
Isaji
Motomi
M
Yamane
Tomoko
T
Iwama
Tomonori
T
D003542:Cystathionine gamma-Lyase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D003545:Cysteine / Q000378:metabolism* / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013379:Substrate Specificity
D003542:Cystathionine gamma-Lyase; D003545:Cysteine