title: Somatostatin suppresses ghrelin secretion from the rat stomach.
abstract: Ghrelin is an acylated peptide that stimulates food intake and the secretion of growth hormone. While ghrelin is predominantly synthesized in a subset of endocrine cells in the oxyntic gland of the human and rat stomach, the mechanism regulating ghrelin secretion remains unknown. Somatostatin, a peptide produced in the gastric oxyntic mucosa, is known to suppress secretion of several gastrointestinal peptides in a paracrine fashion. By double immunohistochemistry, we demonstrated that somatostatin-immunoreactive cells contact ghrelin-immunoreactive cells. A single intravenous injection of somatostatin reduced the systemic plasma concentration of ghrelin in rats. Continuous infusion of somatostatin into the gastric artery of the vascularly perfused rat stomach suppressed ghrelin secretion in both dose- and time-dependent manner. These findings indicate that ghrelin secretion from the stomach is regulated by gastric somatostatin.
authors.affiliation: Department of Internal Medicine, Oita Medical University, Oita 879-5593, Japan.
authors.forename: Mitsushi
authors.initials: M
authors.lastname: Shimada
authors.forename: Yukari
authors.initials: Y
authors.lastname: Date
authors.forename: Muhtashan S
authors.initials: MS
authors.lastname: Mondal
authors.forename: Koji
authors.initials: K
authors.lastname: Toshinai
authors.forename: Takuya
authors.initials: T
authors.lastname: Shimbara
authors.forename: Kyoko
authors.initials: K
authors.lastname: Fukunaga
authors.forename: Noboru
authors.initials: N
authors.lastname: Murakami
authors.forename: Mikiya
authors.initials: M
authors.lastname: Miyazato
authors.forename: Kenji
authors.initials: K
authors.lastname: Kangawa
authors.forename: Hironobu
authors.initials: H
authors.lastname: Yoshimatsu
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Matsuo
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Nakazato
mesh_terms: D000818:Animals; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005753:Gastric Mucosa / Q000378:metabolism*; D054439:Ghrelin; D007150:Immunohistochemistry; D008297:Male; D015282:Octreotide / Q000494:pharmacology; D036361:Peptide Hormones / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D010477:Perfusion; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000494:pharmacology* / Q000502:physiology*; D013270:Stomach / Q000187:drug effects; D013997:Time Factors
chemical_list: D054439:Ghrelin; D036361:Peptide Hormones; D013004:Somatostatin; D015282:Octreotide