title: [Preparation and identification of polyclonal antiserum against angiotensinogen].
abstract: For studying the expression and distribution of angiotensinogen (AGT), the C-teminus of rat AGT gene was expressed in E.coli. Rabbits were immunized with expressed AGT protein and sera from different rabbits were raised. ELISA showed a high titre (1:25600) of the antiserum. With the antiserum, Western blotting recognized not only the prokaryotic expressed AGT, but also the endogenous AGT protein in liver tissue of both rats and humans. Using this antiserum, immunohistochemistry showed the expression of AGT protein in islet cells of human pancreas as well as in epithelium of human bile duct. These results suggest that the prokaryotic expressed AGT protein is an effective immunogen for the preparation of anti-AGT antiserum. Our present work provides an important tool for study of the pathophysiological role of AGT as well as local renin-angiotensin system.
authors.affiliation: Department of Aerospace Physiology, the Fourth Military Medical University, Xi  an 710032.
authors.forename: Qing-Jun
authors.initials: QJ
authors.lastname: Meng
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Wen-Hong
authors.initials: WH
authors.lastname: Zhang
authors.forename: Le-Ning
authors.initials: LN
authors.lastname: Zhang
authors.forename: Li-Fan
authors.initials: LF
authors.lastname: Zhang
mesh_terms: D000808:Angiotensinogen / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007106:Immune Sera / Q000096:biosynthesis* / Q000276:immunology; D007114:Immunization; D011817:Rabbits; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D012084:Renin-Angiotensin System / Q000502:physiology
chemical_list: D000911:Antibodies, Monoclonal; D007106:Immune Sera; D011994:Recombinant Proteins; D000808:Angiotensinogen