Microarray workshop on aging.
The advent of microarrays in studying gene expression in aging has created tremendous excitement due to its potential for uncovering molecular mechanisms of aging and age-related disease. However, the appropriate implementation of this technology in the science of aging requires serious attention to methodological detail and statistical rigor. This report highlights discussions from the microarray workshop on aging held at the First Conference on Functional Genomics of Aging in Seville, Spain. The topics discussed by the participants included technical issues relating to the printing of arrays, RNA isolation, cDNA labeling and hybridization, optimal design of microarray experiments, and statistical analysis of these data. Microarray analysis of complex tissues through the use of laser capture microdissection was also discussed.
Nair
Prakash N
PN
Department of Physiology and South Texas Centers for Biology in Medicine, University of Texas Health Science Center in San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA. nairp@uthscsa.edu <nairp@uthscsa.edu>
Golden
Tamara
T
Melov
Simon
S
D000375:Aging / Q000235:genetics*; D000818:Animals; D003627:Data Interpretation, Statistical; D005456:Fluorescent Dyes; D020869:Gene Expression Profiling / Q000379:methods / Q000706:statistics & numerical data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000706:statistics & numerical data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results
D005456:Fluorescent Dyes; D012333:RNA, Messenger