title: Molecular cloning and characterization of TPP36 and its isoform TPP32, novel substrates of Abl tyrosine kinase.
abstract: We have molecularly cloned TPP36, a novel 36 kDa protein with 281 amino acids that was identified as a protein phosphorylated in B progenitor cells following stimulation with pervanadate/H(2)O(2). Analysis with anti-TPP36 antiserum revealed that TPP36 was expressed ubiquitously and had an isoform with 236 amino acids, designated TPP32. TPP36/32 were localized mainly in cytoplasm despite the presence of a typical nuclear localization signal sequence. These proteins were phosphorylated preferentially by Abl among a panel of tyrosine kinases examined. Phosphorylation of tyrosine 120 in TPP36/32 led to an apparent mobility shift in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, suggesting conformational change in the phosphorylated protein. Thus, TPP36/32 appear to be novel substrates of Abl tyrosine kinase.
authors.affiliation: Department of Gastroenterology and Hepatology, Tokyo Medical and Dental University Graduate School, Japan.
authors.forename: Kiichiro
authors.initials: K
authors.lastname: Tsuchiya
authors.forename: Youhei
authors.initials: Y
authors.lastname: Kawano
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kojima
authors.forename: Kisaburo
authors.initials: K
authors.lastname: Nagata
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Takao
authors.forename: Masato
authors.initials: M
authors.lastname: Okada
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Shinohara
authors.forename: Kazushige
authors.initials: K
authors.lastname: Maki
authors.forename: Noriko
authors.initials: N
authors.lastname: Toyama-Sorimachi
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Miyasaka
authors.forename: Mamoru
authors.initials: M
authors.lastname: Watanabe
authors.forename: Hajime
authors.initials: H
authors.lastname: Karasuyama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016374:Oncogene Proteins v-abl / Q000378:metabolism*; D009990:Oryzias; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D016315:Proto-Oncogene Proteins c-abl; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014981:Xenopus
chemical_list: C473117:Cdv3 protein, mouse; D016374:Oncogene Proteins v-abl; D010750:Phosphoproteins; D020033:Protein Isoforms; D011505:Protein-Tyrosine Kinases; D016315:Proto-Oncogene Proteins c-abl