Antisense suppression of collagen VI synthesis results in reduced expression of collagen I in normal human osteoblast-like cells.
A transient increase in collagen VI expression precedes the accumulation of collagen I associated with interleukin-4 (IL-4)-induced mineralization in human osteoblast-like cells. Transfection with an antisense oligonucleotide specific for alpha1(VI) collagen mRNA was shown to attenuate mRNA levels of collagens VI and I. Incubating IL-4 treated cells with anti-collagen VI antiserum decreased expression of alpha1(I) mRNA. The results suggest that collagen VI may regulate collagen I expression in the early phase of IL-4-induced mineralization.
Harumiya
Satoru
S
Department of Nutrition, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo 173-0015, Japan. harumiya.csi@tmd.ac.jp
Gibson
Mark A
MA
Koshihara
Yasuko
Y
D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics*; D024142:Collagen Type VI / Q000235:genetics*; D020868:Gene Silencing; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D016376:Oligonucleotides, Antisense / Q000378:metabolism*; D010006:Osteoblasts / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D024042:Collagen Type I; D024142:Collagen Type VI; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D015847:Interleukin-4