title: Glutathione S-transferase isoenzymatic response to aging in rat cerebral cortex and cerebellum.
abstract: Aging is associated with increased oxidant generation. One mechanism involved in the defense of oxidative products is the family of glutathione transferases (GST). We have analyzed the activity, distribution and expression of GSTP1 and GSTA4 isoenzymes in the cerebral cortex and cerebellum of young, adult and aged rats. The total GST activity, measured with the universal substrate 1-chloro-2,4-dinitrobenzene (CDNB), increased only with the maturation process; however GSTA4 activity, using the specific substrate 4-hydroxynonenal (HNE), did show an age-dependent increase in both brain regions. Cellular location of GSTA4 in astrocytes was not changed except for young cerebral cortex and adult/aged cerebellum that also showed immunoreactivity in layer III pyramidal neurons and Bergman radial glia, respectively. Distribution of GSTP1 was similar among groups and only an increased number of positive oligodendrocytes was found in the Purkinje and granular layer of adult/aged cerebellum. The GSTA4 and GSTP1 expression increased from young to adult/aged brain and GSTA4 even augmented in the aged cerebral cortex. These results suggest a GST isoenzymatic response with aging, but above all with the maturation process.
authors.affiliation: Department of Experimental Biology, University of Jaén, Paraje Las Lagunillas s/n, 23071, Jaén, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Martínez-Lara
authors.forename: Eva
authors.initials: E
authors.lastname: Siles
authors.forename: Raquel
authors.initials: R
authors.lastname: Hernández
authors.forename: Ana R
authors.initials: AR
authors.lastname: Cañuelo
authors.forename: M
authors.initials: M
authors.lastname: Luisa del Moral
authors.forename: Ana
authors.initials: A
authors.lastname: Jiménez
authors.forename: Santos
authors.initials: S
authors.lastname: Blanco
authors.forename: Juan C
authors.initials: JC
authors.lastname: López-Ramos
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Esteban
authors.forename: Juan A
authors.initials: JA
authors.lastname: Pedrosa
authors.forename: M Angeles
authors.initials: MA
authors.lastname: Peinado
mesh_terms: D000367:Age Factors; D000375:Aging / Q000502:physiology*; D000818:Animals; D001253:Astrocytes / Q000201:enzymology*; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000378:metabolism; D002531:Cerebellum / Q000201:enzymology*; D002540:Cerebral Cortex / Q000201:enzymology*; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000378:metabolism*; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D009836:Oligodendroglia / Q000201:enzymology*; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; C105760:GstA protein, bacteria; D047908:Intracellular Signaling Peptides and Proteins; D007527:Isoenzymes; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; C496558:Gstp1 protein, rat