title: Regulation of anti-phosphatidylserine antibodies.
abstract: The degree of heavy chain (H) editing, the types of Vkappa editors, and the pattern of Jkappa usage are correlated with a range of the affinity of anti-DNA. This range was determined by the number and location of arginine (R) residues in the VH. We, here, changed a key arginine residue in the VH of anti-DNA transgene to glycine, which sharply reduces the affinity for dsDNA. However, complete reversion of this anti-DNA to germline enhances the affinity for phosphatidylserine (PS). The B cells of this low-affinity anti-DNA and anti-PS transgenic mouse are tightly regulated by receptor editing. Thus, anti-PS B cells are another example of a constitutive self-antigen regulated in the bone marrow.
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Yufei
authors.initials: Y
authors.lastname: Jiang
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Marko
authors.initials: M
authors.lastname: Radic
authors.forename: Eline Luning
authors.initials: EL
authors.lastname: Prak
authors.forename: Martin
authors.initials: M
authors.lastname: Weigert
mesh_terms: D000818:Animals; D020296:Animals, Congenic; D017152:Antibodies, Antiphospholipid / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001324:Autoantigens; D004247:DNA / Q000378:metabolism; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D006825:Hybridomas / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007145:Immunoglobulin kappa-Chains / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D010718:Phosphatidylserines / Q000276:immunology*; D017393:RNA Editing; D017634:Self Tolerance; D055672:Static Electricity
chemical_list: D017152:Antibodies, Antiphospholipid; D001324:Autoantigens; D007143:Immunoglobulin Heavy Chains; D007145:Immunoglobulin kappa-Chains; D010718:Phosphatidylserines; D004247:DNA