title: Immunocytolocalization of tryptophan decarboxylase in Catharanthus roseus hairy roots.
abstract: We investigated the intracellular distribution of tryptophan decarboxylase (TDC) (EC 4.1.1.28) in Catharanthus roseus hairy roots using immunofluorescence and immunogold techniques. TDC was detected by immunofluorescence localization in the cytosol and in the apoplastic region of the meristematic cells of the roots, with a slight enrichment in the epidermal cells of the root cap and in the meristematic region. In the enlargement zone, TDC was localized only in the first three layers of the cortex. In the maturation zone, the enzyme was not present. Immunogold studies confirmed that the enzyme was localized in the cytosol of the meristematic region, and intense gold labeling was found in the apoplastic zone. A protein fraction isolated from the apoplastic zone and assayed for TDC activity showed high activity.
authors.affiliation: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de investigacíon Científica de Yucatán, Calle 43, número 130, CP 97200, Mérida, Yucatán, México.
authors.forename: Oscar A
authors.initials: OA
authors.lastname: Moreno-Valenzuela
authors.forename: Yereni
authors.initials: Y
authors.lastname: Minero-García
authors.forename: Ligia
authors.initials: L
authors.lastname: Brito-Argáez
authors.forename: Eloísa
authors.initials: E
authors.lastname: Carbajal-Mora
authors.forename: Olga
authors.initials: O
authors.lastname: Echeverría
authors.forename: Gerardo
authors.initials: G
authors.lastname: Vázquez-Nin
authors.forename: Víctor M
authors.initials: VM
authors.lastname: Loyola-Vargas
mesh_terms: D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000737:chemistry* / Q000378:metabolism*; D030008:Catharanthus / Q000737:chemistry / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D046508:Culture Techniques / Q000379:methods; D004789:Enzyme Activation; D005455:Fluorescent Antibody Technique; D018517:Plant Roots / Q000737:chemistry / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: D001142:Aromatic-L-Amino-Acid Decarboxylases