Effects of voluntary resistance exercise on heme biosynthesis in rats given glucocorticoid-injections.
To identify the effects of a tower-climbing exercise on bone marrow heme biosynthesis and hematological status in rats given glucocorticoid-injections as a model of aging, 29 male Sprague-Dawley rats, 10 weeks of age, were assigned to three groups: a saline control (C, n = 9), a glucocorticoid-sedentary (GS, n = 10) group, and a glucocorticoid-exercise (GE, n = 10) group. The GS and GE groups were given 2 mg/kg prednisolone daily, and the C group was given 2 ml of saline daily, subcutaneously. Each group was meal-fed commercial rat chow isoenergetically and given free access to water for 8 weeks. The GE group were allowed to climb a 200-cm tower to drink water from a bottle set at the top of it. Weight gain during the 8-week experimental period was greater in the C group than in the GS and GE groups. The gastrocnemius and tibialis anterior muscles were heavier in the C group than in other groups. The hematological parameters were not influenced by glucocorticoid administration with or without climbing exercise. Bone marrow delta-aminolevulinic acid dehydratase activity was significantly higher in the GE group than in the C group. These results suggest that 8 weeks of climbing exercise increases heme biosynthesis without alteration of hematological status in rats given glucocorticoid-injections. Resistance exercise may be a preventive therapy for iron-deficiency anemia associated with aging.
Kang
Hyung-Sook
HS
Graduate School of East-West Medical Science, Kyung-Hee University, Seoul, Korea.
Matsuo
Tatsuhiro
T
Choue
Ryo-Won
RW
Suzuki
Masashige
M
D000375:Aging; D000818:Animals; D001835:Body Weight; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D005938:Glucocorticoids / Q000494:pharmacology; D006418:Heme / Q000096:biosynthesis*; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000502:physiology; D009929:Organ Size; D010805:Physical Conditioning, Animal / Q000502:physiology*; D000623:Porphobilinogen Synthase / Q000378:metabolism; D011239:Prednisolone / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D005938:Glucocorticoids; D017392:Thiobarbituric Acid Reactive Substances; D006418:Heme; D011239:Prednisolone; D000623:Porphobilinogen Synthase