Cell interaction studies of PLA-MePEG nanoparticles.
Poly(D,L-lactic acid)-methoxypoly(ethylene glycol) (PLA-MePEG) copolymers were synthesized by ring-opening polymerization of D,L-lactide in the presence of MePEG of different molecular weights and stannous octoate as the catalyst. The chemical composition of the diblock-copolymer PLA-MePEG was confirmed by 1H-NMR and the molecular weight and distribution were assessed by gel permeation chromatography. Nanoparticles containing Nile red as a fluorescent dye were prepared using poly(D,L-lactic acid) (PLA), blends of PLA and PLA-MePEG or PLA-MePEG alone. Incubation of nanoparticles with human blood monocytes was performed in serum or in PBS and the cell-associated fluorescence was analyzed by flow cytometry. In serum, a protective effect was obtained and the interaction of particles with mononuclear leukocytes decreased to 40%.
Nguyen
Cung An
CA
School of Pharmacy, University of Geneva, 30, Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.
Allémann
Eric
E
Schwach
Grégoire
G
Doelker
Eric
E
Gurny
Robert
R
D001672:Biocompatible Materials; D001769:Blood / Q000378:metabolism; D002850:Chromatography, Gel / Q000295:instrumentation / Q000379:methods; D004337:Drug Carriers; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008863:Microspheres; D008970:Molecular Weight; D036103:Nanotechnology; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties
D001672:Biocompatible Materials; D004337:Drug Carriers; D011091:Polyesters; D011108:Polymers; D011092:Polyethylene Glycols; C033616:poly(lactide)