title: Trichothecene nonproducer Gibberella species have both functional and nonfunctional 3-O-acetyltransferase genes.
abstract: The trichothecene 3-O-acetyltransferase gene (FgTri101) required for trichothecene production by Fusarium graminearum is located between the phosphate permease gene (pho5) and the UTP-ammonia ligase gene (ura7). We have cloned and sequenced the pho5-to-ura7 regions from three trichothecene nonproducing Fusarium (i.e., F. oxysporum, F. moniliforme, and Fusarium species IFO 7772) that belong to the teleomorph genus Gibberella. BLASTX analysis of these sequences revealed portions of predicted polypeptides with high similarities to the TRI101 polypeptide. While FspTri101 (Fusarium species Tri101) coded for a functional 3-O-acetyltransferase, FoTri101 (F. oxysporum Tri101) and FmTri101 (F. moniliforme Tri101) were pseudogenes. Nevertheless, F. oxysporum and F. moniliforme were able to acetylate C-3 of trichothecenes, indicating that these nonproducers possess another as yet unidentified 3-O-acetyltransferase gene. By means of cDNA expression cloning using fission yeast, we isolated the responsible FoTri201 gene from F. oxysporum; on the basis of this sequence, FmTri201 has been cloned from F. moniliforme by PCR techniques. Both Tri201 showed only a limited level of nucleotide sequence similarity to FgTri101 and FspTri101. The existence of Tri101 in a trichothecene nonproducer suggests that this gene existed in the fungal genome before the divergence of producers from nonproducers in the evolution of Fusarium species.
authors.affiliation: Laboratory for Remediation Research, Plant Science Center, RIKEN, Wako, Saitama 351-0198, Japan. mkimura@postman.riken.go.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Kimura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tokai
authors.forename: Gentaro
authors.initials: G
authors.lastname: Matsumoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Fujimura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hamamoto
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Yoneyama
authors.forename: Takehiko
authors.initials: T
authors.lastname: Shibata
authors.forename: Isamu
authors.initials: I
authors.lastname: Yamaguchi
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D022761:Gene Transfer, Horizontal; D005874:Gibberella / Q000201:enzymology*; D008969:Molecular Sequence Data; D028061:Phosphate Transport Proteins / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D026801:Synteny; D014255:Trichothecenes / Q000378:metabolism*
chemical_list: D028061:Phosphate Transport Proteins; D014255:Trichothecenes; C055831:phosphate permease; D000123:Acetyltransferases; C110205:trichothecene 3-O-acetyltransferase