title: Production of CTX-M-3 extended-spectrum beta-lactamase and IMP-1 metallo beta-lactamase by five Gram-negative bacilli: survey of clinical isolates from seven laboratories collected in 1998 and 2000, in the Kinki region of Japan.
abstract: The aim of this study was to research the distribution in the Kinki region of Japan of Enterobacteriaceae and Pseudomonas aeruginosa that produce extended-spectrum beta-lactamase (ESBL) and metallo beta-lactamase (MBL). One thousand isolates, 200 of each of four enterobacterial species (i.e. Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae and Serratia marcescens) and 200 of P. aeruginosa, were collected from seven different laboratories during two 2 month periods, one in 1998 and one in 2000. A double-disc synergy test (DDST) and 2-mercaptopropionic acid inhibition test (2-MPAT) were used to confirm beta-lactamase-producing isolates. The DDST was positive for one isolate of E. coli, five of K. pneumoniae, two of E. cloacae and 14 of S. marcescens. The 2-MPAT was positive for five isolates of S. marcescens and two of P. aeruginosa. We identified the beta-lactamase type of each isolate by molecular confirmatory tests (isoelectric focusing, PCR and DNA sequencing): CTX-M-3 ESBLs (three isolates of K. pneumoniae, two of E. cloacae and 13 of S. marcescens), CTX-M-2 ESBL (one isolate of K. pneumoniae), SHV-12 ESBLs (one isolate of E. coli and one of S. marcescens), CTX-M-3 and SHV-12 combination ESBL (one isolate of K. pneumoniae) and IMP-1 MBLs (five isolates of S. marcescens and two of P. aeruginosa). In conclusion, many species of Gram-negative bacilli that produce CTX-M-3 ESBLs and IMP-1 MBLs were disseminated widely in different hospitals of the Kinki region of Japan. Therefore, monitoring of laboratory bacterial ecology seems important to stop the spread of these strains through nosocomial outbreaks.
authors.affiliation: Division of Clinical Microbiology, Department of Clinical Laboratory, Wakayama Rosai Hospital, 435 Koya, Wakayama, Japan.
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Yamasaki
authors.forename: Masaru
authors.initials: M
authors.lastname: Komatsu
authors.forename: Tomonari
authors.initials: T
authors.lastname: Yamashita
authors.forename: Koichi
authors.initials: K
authors.lastname: Shimakawa
authors.forename: Toshiro
authors.initials: T
authors.lastname: Ura
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Nishio
authors.forename: Kaori
authors.initials: K
authors.lastname: Satoh
authors.forename: Ryoudou
authors.initials: R
authors.lastname: Washidu
authors.forename: Shohiro
authors.initials: S
authors.lastname: Kinoshita
authors.forename: Masanori
authors.initials: M
authors.lastname: Aihara
mesh_terms: D003428:Cross Infection / Q000201:enzymology / Q000453:epidemiology / Q000382:microbiology; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000302:isolation & purification; D006306:Health Surveys; D006801:Humans; D007564:Japan / Q000453:epidemiology; D007755:Laboratories, Hospital / Q000706:statistics & numerical data / Q000639:trends*; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: C417271:beta-lactamase CTX-M-3; C409201:beta-lactamase IMP-1; D001618:beta-Lactamases