title: Hemolytic steroid disulfates from the far eastern starfish Pteraster pulvillus.
abstract: Six steroidal ophiuroid-type disulfates, namely, disodium salts of (20R)-cholest-5-ene-3alpha,4beta,21-triol 3, 21-disulfate (1), (20R)-5alpha-cholestane-3alpha,4beta,21-triol 3,21-disulfate (2), (20R)-5alpha-cholestane-2beta,3alpha,21-triol 3,21-disulfate (3), and (20R)-5alpha-cholestane-3alpha,21-diol 3,21-disulfate (4), the dityrammonium salt of (20R)-5alpha-cholestane-3alpha,21-diol 3,21-disulfate (5), and a mixture of sodium and tyrammonium salts (1:1) of (20R)-cholest-5-ene-3alpha,21-diol 3,21-disulfate (6), have been isolated from the ethanolic extracts of Far Eastern starfish Pteraster pulvillus. Steroids 2 and 3 are new compounds. Steroids 1, 2, and 4-6 showed hemolytic activity to mouse erythrocytes with HC(50) values of 8.0 x 10(-5), 4.5 x 10(-5), 1.0 x 10(-5), 1.8 x 10(-5), and 3.3 x 10(-5) M, respectively.
authors.affiliation: Pacific Institute of Bioorganic Chemistry of the Far East Branch of the Russian Academy of Science, Vladivostok-22, Russian Federation.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Ivanchina
authors.forename: Alla A
authors.initials: AA
authors.lastname: Kicha
authors.forename: Anatoly I
authors.initials: AI
authors.lastname: Kalinovsky
authors.forename: Pavel S
authors.initials: PS
authors.lastname: Dmitrenok
authors.forename: Valentin A
authors.initials: VA
authors.lastname: Stonik
mesh_terms: D000818:Animals; D002777:Cholestanols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D012426:Russia; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013215:Starfish / Q000737:chemistry*; D013237:Stereoisomerism; D013431:Sulfates / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D002777:Cholestanols; D013431:Sulfates