Genetic diversity and phylogenetic analysis of glycoprotein 5 of European-type porcine reproductive and respiratory virus strains in Spain.
The gene encoding glycoprotein 5 (ORF5) of 21 porcine reproductive and respiratory syndrome virus (PRRSV) isolates from Spain and two European-type vaccines currently available in that country were analysed using RT-PCR and sequencing. Sequences were then compared with other European-type sequences available through GenBank. Results showed percentages of similarity to Lelystad virus (LV), which, in most cases, were below 90 %. In contrast, two strains were very similar (>99 %) to a PRRSV variant from the Czech Republic. Evolutionary trees showed three types of strains: one grouped old Spanish sequences; a second grouped isolates from this study together with two Czech variant strains; and the third comprised other GenBank sequences. Regarding the predicted protein sequences, some isolates from this study showed a low degree of similarity to LV (below 50 %) and most of the strains examined had additional N-linked glycosylation sites compared to LV. These results provide evidence of the existence of variant PRRSV strains in Spain with characteristics that may be advantageous for immune evasion.
Mateu
Enric
E
Centre de Recerca en Sanitat Animal (CReSA), Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.|Departament de Sanitat i Anatomia Animals, Edifici V, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Martín
Marga
M
Centre de Recerca en Sanitat Animal (CReSA), Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.|Departament de Sanitat i Anatomia Animals, Edifici V, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Vidal
Dolors
D
Departament de Sanitat i Anatomia Animals, Edifici V, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
D000818:Animals; D001665:Binding Sites; D014644:Genetic Variation; D006031:Glycosylation; D008969:Molecular Sequence Data; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis*; D012689:Sequence Homology, Nucleic Acid; D013030:Spain; D013552:Swine / Q000097:blood / Q000821:virology*; D014759:Viral Envelope Proteins / Q000235:genetics*; D014765:Viral Vaccines / Q000235:genetics
D012367:RNA, Viral; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C114789:glycoprotein 5, PRRSV