OsRALyase1, a putative F-box protein identified in rice, Oryza sativa, with enzyme activity identical to that of wheat RALyase.
A rice gene, OsRALyase1, encoding a product similar to wheat ribosomal RNA apurinic site specific lyase (RALyase), was isolated and expressed in vitro. An open reading frame of the gene predicted a protein of 476 amino acid residues with 75% identity to RALyase and contained an F-box-like motif in its amino terminal region. The rice gene product expressed in a wheat-germ protein expression system had the same characteristics as its wheat counterpart, cleaving a specific depurinated site of the 28S rRNA sarcin-ricin domain.
Ito
Yasuhiro
Y
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Ozawa
Akihiko
A
Sawasaki
Tatsuya
T
Endo
Yaeta
Y
Ochi
Kozo
K
Tozawa
Yuzuru
Y
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D004722:Endoribonucleases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000201:enzymology*
D012339:RNA, Ribosomal, 28S; D004722:Endoribonucleases; C402789:ribosomal RNA apurinic site specific lyase