[Determination of sucralose in foods by HPLC using pre-column derivatization].
The development of a sensitive pre-column derivatization high-performance liquid chromatography (HPLC) method for determination of sucralose is reported. Sucralose is converted into a strongly ultraviolet (UV)-absorbing derivative, possessing strong absorption at 260 nm, by treatment with p-nitrobenzoyl chloride (PNBCl). Homogenized samples were dialyzed and washed with a Bond Elut ENV cartridge, then the eluate was evaporated to dryness and the residue was derivatized. Subsequently, the sucralose derivative was purified with hexane-ethyl actate (9:1) in a silica cartridge, and then the sucralose derivative was eluted with acetone. HPLC was performed on a phenyl column, using acetonitrile-water (73:27) as a mobile phase with UV detection (260 nm). The calibration curve was linear in the range of 1 microgram/mL to 50 micrograms/mL of sucralose. The recoveries of sucralose from eight kinds of foods spiked at the levels of 0.20 and 0.05 g/kg of sucralose were more than 76.2% with SD values in the range from 0.90% to 4.31%. The quantitative limit of the developed method was 0.005 g/kg for sucralose in samples.
Nojiri
Shuko
S
Tama Branch Laboratory, Tokyo Metropolitan Research Laboratory of Public Health: 3-16-25, Shibasaki-cho, Tachikawa, Tokyo 190-0023, Japan.
Nakazato
Mitsuo
M
Kasuya
Yoko
Y
Takano
Ichiro
I
Oishi
Mitsuo
M
Yasuda
Kazuo
K
Suzuki
Sukeji
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D013395:Sucrose / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry
D013395:Sucrose; C026285:trichlorosucrose