title: Increased inducible nitric oxide synthase expression and nitric oxide concentration in patients with aplastic anemia.
abstract: Nitric oxide (NO) is a biological mediator that is synthesized from L-arginine by the nitric oxide synthase (NOS) family. We investigated the expression of iNOS in bone marrow (BM) mononuclear cells (MNCs) using a reverse transcriptase polymerase chain reaction (RT-PCR) assay and the concentration of NO from BM serum by measuring the metabolite NO(2)(-) in 13 patients with aplastic anemia (AA) compared with 10 normal controls who were donors for allogeneic bone marrow transplantation (BMT). All samples of BM MNCs in patients with AA expressed iNOS mRNA, but iNOS was not expressed in patients who were treated successfully with allogeneic BMT. Normal control samples and samples from leukemia patients who had bone marrow aplasia after chemotherapy did not show significant iNOS expression. When we measured the density of bands for both iNOS and beta(2)-microglobin expressed as the iNOS/beta(2)-microglobin density ratio, there was a significant difference in the ratio between AA and normal controls (0.88+/-0.15 vs 0.26+/-0.05, P<0.001). The BM serum NO(2)(-) concentration in the patients with AA was significantly higher than that of normal controls (88.1+/-32.8 microM vs 48.8+/-8.6 microM, P=0.002). In addition, there was a significant correlation between the NO(2)(-) concentration and the calculated iNOS/beta(2)-microglobin density ratio (r=0.567, P=0.01). These findings suggest that upregulation of iNOS expression for local NO production may contribute in part to the pathogenesis of AA.
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-Ku, Gwangju, South Korea.|Genome Research Center for Hematopoietic Diseases, Chonnam National University Hospital, Gwangju, South Korea.
authors.forename: I-J
authors.initials: IJ
authors.lastname: Chung
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-Ku, Gwangju, South Korea.|Genome Research Center for Hematopoietic Diseases, Chonnam National University Hospital, Gwangju, South Korea.
authors.forename: J-J
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: Genome Research Center for Hematopoietic Diseases, Chonnam National University Hospital, Gwangju, South Korea.
authors.forename: C-E
authors.initials: CE
authors.lastname: Nam
authors.affiliation: Genome Research Center for Hematopoietic Diseases, Chonnam National University Hospital, Gwangju, South Korea.
authors.forename: H N
authors.initials: HN
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-Ku, Gwangju, South Korea.
authors.forename: Y-K
authors.initials: YK
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-Ku, Gwangju, South Korea.
authors.forename: M-R
authors.initials: MR
authors.lastname: Park
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-Ku, Gwangju, South Korea.
authors.forename: S-H
authors.initials: SH
authors.lastname: Cho
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-Ku, Gwangju, South Korea. ijchung@chonnam.ac.kr.|Genome Research Center for Hematopoietic Diseases, Chonnam National University Hospital, Gwangju, South Korea. ijchung@chonnam.ac.kr.
authors.forename: H-J
authors.initials: HJ
authors.lastname: Kim
mesh_terms: D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000201:enzymology* / Q000209:etiology / Q000235:genetics*; D001853:Bone Marrow / Q000098:blood supply; D001854:Bone Marrow Cells / Q000201:enzymology / Q000378:metabolism; D016022:Case-Control Studies; D004790:Enzyme Induction; D015870:Gene Expression; D006801:Humans; D009569:Nitric Oxide / Q000097:blood*; D019001:Nitric Oxide Synthase / Q000235:genetics* / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000032:analysis; D018709:Statistics, Nonparametric
chemical_list: D012333:RNA, Messenger; D009569:Nitric Oxide; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II