[Effect of tetracycline on the hepatitis C virus propagation and levels of cytokine mRNA in infected SW-13 and MT-4 cells].
An experimental HCV-infection model was used in vitro to study the influence of tetracycline on the reproduction of the HCV-infection accompanied by a simultaneous analysis, by using the methods of reverse transcription and polymerase chain reaction (RT-PCR), of the mRNA activity, i.e. alpha-IFN, gamma-IFN, 1 beta-IFN, IL-2, IL-4, IL-6, IL-6, IL-10, IL-12, IL-18 and TNF-alpha. The study was made with the SW-13 long-term cellular cultures (human adrenocarcinoma cells) and MT-4 cell cultures (human lymphoblastoid cells) infected by HCV. An analysis of the obtained data is indicative, in both examined cellular cultures, of a differently-oriented induction-suppression reaction of cytokine mRNAs. Suppression of the activity of the investigated cytokine mRNAs, except for mRNAs IL-18 and TNF-alpha, was observed in the SW-13 cellular culture; while, as for the MT-4 cellular culture, the activity of all studied cytokine mRNAs was pointed out. The results testify to that tetracycline should be studied more actively in experimental infections caused by HCV in vivo.
Narovlianskiĭ
A N
AN
Vershinina
M Iu
MIu
Deriabin
P G
PG
Mezentseva
M V
MV
Ershov
F I
FI
D002460:Cell Line; D016207:Cytokines / Q000032:analysis / Q000378:metabolism*; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D016174:Hepacivirus / Q000187:drug effects* / Q000502:physiology; D006526:Hepatitis C / Q000188:drug therapy / Q000821:virology; D006801:Humans; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013752:Tetracycline / Q000494:pharmacology*
D016207:Cytokines; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D012367:RNA, Viral; D013752:Tetracycline