Identification of a hydroxy substituted calamenene--a sesquiterpene associated with wound reactions in non-infected xylem of Tilia spp.
Xylem of lime trees (Tilia spp.) with wound reactions was structurally investigated by scanning (SEM) and transmission electron microscopy (TEM) as well as chemically analyzed by direct thermal desorption-gas chromatography-mass spectrometry (DTD-GC-MS). Wound reactions in the outer xylem lead to distinct discolorations around the wound. Within a 4-week response no fungal infection occurred in discoloured xylem. At the fine structural level, wound reactions become primarily visible as the secretion of dark-staining substances from parenchyma cells into lumens of vessels and fibres. With increasing reaction time vessels aggregate large amounts of secretion products, whereas in fibres wall-associated linings are formed and the inner secondary wall appears incrusted. After 2-3 months a narrow, greenish-brown boundary developed at the transition between the discoloured outer and the unchanged inner xylem. This green-brown boundary layer remained non-infected also in older wounds. DTD-GC-MS analyses revealed that the sesquiterpene Hydroxycalamenene represents a key substance of wound reactions in non-infected lime trees. Other substances such as fatty acids or their esters and coniferyl aldehydes or their derivatives were also found. TEM investigations of the samples after DTD-GC-MS showed less pronounced cell wall-attached linings in fibres as well as reduced incrustation of inner secondary walls. The massive deposits in the vessel lumens remained unchanged. The role of these wound reaction products and their ways of synthesis are discussed.
Melcher
Eckhard
E
Federal Research Centre for Forestry and Forest Products, Institute for Wood Biology and Wood Protection, Leuschnerstr. 91, D-21031, Hamburg, Germany.
Jüngel
Peter
P
Möllendorf
Bianca
B
Schmitt
Uwe
U
D005658:Fungi / Q000648:ultrastructure; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010935:Plant Diseases / Q000382:microbiology; D018514:Plant Structures / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013314:Stress, Mechanical; D032384:Tilia / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D013997:Time Factors
D012717:Sesquiterpenes; C473635:hydroxycalamenene