title: Elucidation of signal transduction pathways by transfection of cells with modified oncogenes.
abstract: This chapter will focus on introduction of various wild type (WT) and mutant genes into cells by DNA transfection. Techniques for analysis of the inheritance, expression, and biological effects of the introduced genes will be described. Various strong and weak points about three different techniques of stable gene transfer, including calcium-phosphate DNA precipitation, transfection via liposomes, and transfection via electroporation, will be discussed.
authors.affiliation: Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC, USA.
authors.forename: Linda S
authors.initials: LS
authors.lastname: Steelman
authors.forename: William L
authors.initials: WL
authors.lastname: Blalock
authors.forename: Xiao-Yang
authors.initials: XY
authors.lastname: Wang
authors.forename: Phillip W
authors.initials: PW
authors.lastname: Moye
authors.forename: John T
authors.initials: JT
authors.lastname: Lee
authors.forename: John G
authors.initials: JG
authors.lastname: Shelton
authors.forename: Patrick M
authors.initials: PM
authors.lastname: Navolanic
authors.forename: Julianne M
authors.initials: JM
authors.lastname: Davis
authors.forename: Steven L
authors.initials: SL
authors.lastname: Knapp
authors.forename: Richard A
authors.initials: RA
authors.lastname: Franklin
authors.forename: Martyn K
authors.initials: MK
authors.lastname: White
authors.forename: James A
authors.initials: JA
authors.lastname: McCubrey
mesh_terms: D001940:Breast / Q000166:cytology; D001943:Breast Neoplasms / Q000235:genetics; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009857:Oncogenes / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D014162:Transfection / Q000379:methods*