title: Response of antioxidase in viscera of Pagrosuma major larvae to water soluble fraction of hydrocarbons in No.0 diesel oil.
abstract: Pagrosomus major larvae were exposed to the water-soluble fraction of hydrocarbon in No.0 diesel oil (corresponding to No.2 fuel oil) at concentrations of 0, 0.17, 1.22 and 8.82 mg/L for up to 15 days. Larvae were sampled on days 9 and 15 of the experiment. Supernatants of viscera tissue extractions were assayed for biochemical response in terms of oxidative stress-superoxide dismutase(SOD), activity of selenium-dependant glutathione peroxidase(Se-GPx) and catalase (Ca), and the concentration of reduced glutathione (GSH). On day 9 of exposure, statistically significant dose-related increases in Se-GPx and SOD activity, and GSH concentration were ohserved in all cases except for Se-GPx activity under the highest dosage of hydrocarbon. However, on day 15 of exposure, a similar dose-related response was only observed for Se-GPx activity. GSH concentration decreased and SOD activity showed no statistical difference as compared to controls. However, a significant decrease in compared to day 9 Se-GPx activity and GSH concentration, in contrast to increase SOD activity at day 15 as indicates an accelerated accumulation of H2O2 and potential oxidative damage under long-term exposure of larvae to hydrocarbons. No statistical changes were observed in Ca activity throughout the experiment, possibly owing to the high efficiency of Se-GPx. A recovery experiment was performed on indicating that the response of antioxidants measured tending to return to their control levels. These results prove the function of the antioxidant defense system of the larvae to the water-soluble fraction of hydrocarbons in No.0 diesel oil.
authors.affiliation: Key Laboratory of Marine Environmental Science for Ministry of Education, Environmental Science Research Center, Xiamen University, Xiamen 361005, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Yu
authors.forename: Wei-Yun
authors.initials: WY
authors.lastname: Zheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Weng
authors.forename: Chong-Gang
authors.initials: CG
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002274:Carcinogens, Environmental / Q000009:adverse effects*; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005399:Fishes / Q000502:physiology*; D005742:Gasoline / Q000009:adverse effects*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006838:Hydrocarbons / Q000009:adverse effects*; D007814:Larva; D013482:Superoxide Dismutase / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D000975:Antioxidants; D002274:Carcinogens, Environmental; D005742:Gasoline; D006838:Hydrocarbons; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione