title: Enantioselective binding of S- and R-ofloxacin to various synthetic polynucleotides.
abstract: The binding properties of S- and R-ofloxacin to poly[d(A-T)(2)], poly[d(G-C)(2)] and poly[d(I-C)(2)] were studied by circular dichroism (CD) and various fluorescence techniques. The spectral properties of R-ofloxacin did not change when it was mixed with poly[d(A-T)(2)] and poly[d(I-C)(2)], indicating that R-enantiomer does not interact with these polynucleotides. On the other hand, when S-ofloxacin was mixed with any polynucleotide, or R-enantiomer with poly[d(G-C)(2)], characteristic changes in CD and fluorescence were observed. Therefore, it is clear that enantiomers of ofloxacin selectively recognize B-form DNA. The overall spectral properties of the ofloxacin-polynucleotide complex are similar to those of the norfloxacin-polynucleotide complex [Eur. J. Biochem. 267 (2000) 6018], suggesting that this quinolone also binds in the minor groove of DNA and therefore it may be partially inserted between DNA bases or interact with purine bases.
authors.affiliation: Department of Chemistry, College of Sciences, Yeungnam University Dae-dong, Kyoung-buk Kyoungsan City, 712-749, South Korea.
authors.forename: Hyŭn Jung
authors.initials: HJ
authors.lastname: Hwangbo
authors.forename: Byeong Hwa
authors.initials: BH
authors.lastname: Yun
authors.forename: Jin Soon
authors.initials: JS
authors.lastname: Cha
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Kwon
authors.forename: Seog K
authors.initials: SK
authors.lastname: Kim
mesh_terms: D001665:Binding Sites; D015242:Ofloxacin / Q000737:chemistry* / Q000378:metabolism*; D011119:Polynucleotides / Q000737:chemistry* / Q000378:metabolism*; D013237:Stereoisomerism
chemical_list: D011119:Polynucleotides; D015242:Ofloxacin