Characterization of the simian varicella virus glycoprotein C, which is nonessential for in vitro replication.
The simian varicella virus (SVV) glycoprotein C (gC), which may play an important role in viral pathogenesis, shares extensive homology to the varicella-zoster virus (VZV) gC. The SVV gC gene includes two identical 83 base pair repeat elements which are conserved within the gC genes of epidemiologically distinct SVV isolates. Expression of the gC gene was confirmed by detection of viral gene products. Deletion of the gC gene and replacement with the green fluorescent protein (GFP) gene yields a SVVgC(-)/GFP mutant which replicates as efficiently as wild type virus, demonstrating the SVV gC gene is nonessential for in vitro replication.
Gray
W L
WL
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, U.S.A. graywaynel@uams.edu
Byrne
B H
BH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017353:Gene Deletion; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D018140:Varicellovirus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014779:Virus Replication
D008164:Luminescent Proteins; D014759:Viral Envelope Proteins; D049452:Green Fluorescent Proteins