Spectrofluorimetric and micelle-enhanced spectrofluorimetric methods for the determination of gemfibrozil in pharmaceutical preparations.
A spectrofluorimetric method for the determination of antihyperlipoproteinemic gemfibrozil was developed based on its native fluorescence. This method allows the determination of 0.10-6 microg ml(-1) gemfibrozil in aqueous solution (without using any buffer solution) with excitation and emission wavelengths of 276 and 304 nm, respectively. Detection and quantification limits were 0.03 and 0.10 microg ml(-1), respectively. The fluorescence properties of gemfibrozil in micellar media were also studied. It was shown that in the presence of 0.4% Brij-35 surfactant (pH 4.0, acetic acid-acetate buffer) about 2.4-fold enhancement can be achieved in the fluorescence of this drug. Based on the obtained results, a micelle-enhanced fluorescence method was also developed that is more sensitive than aqueous fluorescence method and has lower detection limit (0.02 microg ml(-1)). Both methods were applied satisfactorily to the determination of gemfibrozil in a commercial pharmaceutical formulation.
Manzoori
Jamshid L
JL
Department of Analytical Chemistry, Faculty of Chemistry, The University of Tabriz, Tabriz, Iran
Amjadi
Mohammad
M
D002138:Calibration; D002214:Capsules; D015248:Gemfibrozil / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D000960:Hypolipidemic Agents / Q000032:analysis*; D007202:Indicators and Reagents; D008823:Micelles; D011208:Powders; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents; D013696:Temperature
D002214:Capsules; D000960:Hypolipidemic Agents; D007202:Indicators and Reagents; D008823:Micelles; D011208:Powders; D012996:Solutions; D013501:Surface-Active Agents; D015248:Gemfibrozil