Analysis of fatty oil in Semen Ziziphi Spinosae by capillary gas chromatography.
A simple and fast capillary gas chromatographic (CGC) method with flame ionization detection is developed for the analysis of fatty oil in Semen Ziziphi Spinosae. After methyl-esterification, eight components are identified by gas chromatography-mass spectrometry. The derivatization condition is investigated in order to validate this method. Palmitic acid and stearic acid are quantitated simultaneously. The limits of detection are 5.024 microg/mL for palmitic acid and 6.957 microg/mL for stearic acid, respectively. The limits of quantitation are 16.76 microg/mL for palmitic acid and 23.19 microg/mL for stearic acid, respectively. The percent recoveries of palmitic and stearic acid are 97.4% and 96.6%. CGC is shown to be a quick and informative tool for the analysis of fatty oil in Semen Ziziphi Spinosae.
Yu-Juan
Li
L
Shenyang Pharmaceutical University, Shenyang 110016, China.
Kai-Shun
Bi
B
Xin-Miao
Liang
L
Hong-Bin
Xiao
X
D002849:Chromatography, Gas / Q000379:methods*; D005227:Fatty Acids / Q000032:analysis*; D010938:Plant Oils / Q000032:analysis*; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D031957:Ziziphus / Q000196:embryology*
D005227:Fatty Acids; D010938:Plant Oils