title: Evidence for the existence of psychrophilic methanogenic communities in anoxic sediments of deep lakes.
abstract: In order to obtain evidence for the existence of psychrophilic methanogenic communities in sediments of deep lakes that are low-temperature environments (4 to 5 degrees C), slurries were first incubated at temperatures between 4 and 60 degrees C for several weeks, at which time they were amended, or not, with an additional substrate, such as cellulose, butyrate, propionate, acetate, or hydrogen, and further incubated at 6 degrees C. Initial methane production rates were highest in slurries preincubated at temperatures between 4 and 15 degrees C, with maximal rates in slurries kept at 6 degrees C. Hydrogen-amended cultures were the only exceptions, with the highest methane production rates at 6 degrees C after preincubation at 30 degrees C.
authors.affiliation: Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Alla N
authors.initials: AN
authors.lastname: Nozhevnikova
authors.forename: Kornelia
authors.initials: K
authors.lastname: Zepp
authors.forename: Francisco
authors.initials: F
authors.lastname: Vazquez
authors.forename: Alexander J B
authors.initials: AJ
authors.lastname: Zehnder
authors.forename: Christof
authors.initials: C
authors.lastname: Holliger
mesh_terms: D000693:Anaerobiosis; D002482:Cellulose / Q000378:metabolism; D003080:Cold Temperature; D003470:Culture Media; D017753:Ecosystem; D019605:Euryarchaeota / Q000254:growth & development*; D005618:Fresh Water / Q000382:microbiology*; D019015:Geologic Sediments / Q000382:microbiology*; D006859:Hydrogen / Q000378:metabolism; D008697:Methane / Q000378:metabolism
chemical_list: D003470:Culture Media; D006859:Hydrogen; D002482:Cellulose; D008697:Methane