Use of shark collagen for cell culture and zymography.
The uses of shark collagen as a matrix for cell culture and as a substrate for zymography were investigated. Fibroblasts were cultured on a gel matrix of shark type I collagen at 30 degrees C. The collagen gel had contracted by 4 days of incubation. Individual fibroblasts were visible against the transparent background of the contracted collagen as long, lean star-shaped cells. The matrix metalloproteinases (MMPs) from fibroblasts secreted from the medium more easily digested shark gelatin than pig gelatin. MMP-2, -9, and that of potential form were recognizable in the zymographic gel of shark gelatin.
Nomura
Yoshihiro
Y
Department of Applied Protein Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo 183-8509, Japan. ny318@cc.tuat.ac.jp
Kitazume
Nobuhide
N
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry / Q000378:metabolism; D005782:Gels / Q000737:chemistry; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D051381:Rats; D012754:Sharks; D013552:Swine
D003470:Culture Media; D005782:Gels; D005780:Gelatin; D003094:Collagen; D020782:Matrix Metalloproteinases