[Column-switching high-performance liquid chromatographic method for the determination of morphine and O6-monoacetylmorphine in urine].
OBJECTIVE
To develop a column-switching high-performance liquid chromatographic method for the determination of morphine and O6-monoacetylmorphine in urine.


METHODS
Urine samples (1.0 ml) were spiked with 1.0 ml borate buffer, after centrifugation, 1.0 ml of supernate were injected directly into an extraction column (YWG C18 33 mm x 5.0 mm, 10 microns). After a washing step with the extraction mobile phase, the retained morphine and O6-monoacetylmorphine were flushed into the analytical column (Lichrospher 100 CN 125 mm x 4.0 mm, 5 microns) with the mobile phase CH3OH-H2O (60:40). The analytical mobile phase is CH3OH-phosphate buffer (pH6.86) (22:78). The UV detector was set at lambda 286 nm.


RESULTS
The method shows excellent linearity from 50 to 1,600 ng/ml for morphine and from 100 to 1,600 ng/ml for O6-monoacetylmorphine. The linear correlation coefficients were > 0.999. The relative standard deviations were < 4%. The limits of detection were 40 ng/ml for both morphine and O6-monoacetylmorphine.


CONCLUSIONS
The method described is sensitive, rapid, reproducible, and simple.
Zhang
Yu-rong
YR
Shanghai Institute of Forensic Science, Shanghai 200083.
Wang
Rong
R
Zhang
Cheng-gong
CG
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006556:Heroin Dependence / Q000652:urine; D006801:Humans; D009020:Morphine / Q000652:urine*; D009022:Morphine Derivatives / Q000652:urine*; D012680:Sensitivity and Specificity
D009022:Morphine Derivatives; D009020:Morphine; C026979:6-O-monoacetylmorphine