title: Purification of fully activated Clostridium botulinum serotype B toxin for treatment of patients with dystonia.
abstract: Clostridium botulinum serotype B toxins 12S and 16S were separated by using a beta-lactose gel column at pH 6.0; toxin 12S passed through the column, whereas toxin 16S bound to the column and eluted with lactose. The fully activated neurotoxin was obtained by applying the trypsin-treated 16S toxin on the same column at pH 8.0; the neurotoxin passed through the column, whereas remaining nontoxic components bound to the column. The toxicity of this purified fully activated neurotoxin was retained for a long period by addition of albumin in the preparation.
authors.affiliation: Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Arimitsu
authors.forename: Kaoru
authors.initials: K
authors.lastname: Inoue
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Sakaguchi
authors.forename: Jaechul
authors.initials: J
authors.lastname: Lee
authors.forename: Yukako
authors.initials: Y
authors.lastname: Fujinaga
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Watanabe
authors.forename: Tohru
authors.initials: T
authors.lastname: Ohyama
authors.forename: Robert
authors.initials: R
authors.lastname: Hirst
authors.forename: Keiji
authors.initials: K
authors.lastname: Oguma
mesh_terms: D000595:Amino Acid Sequence; D001905:Botulinum Toxins / Q000737:chemistry / Q000302:isolation & purification* / Q000627:therapeutic use; D002850:Chromatography, Gel; D004421:Dystonia / Q000188:drug therapy*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data
chemical_list: D001905:Botulinum Toxins