High-throughput protein sequencing.
We have designed and implemented an autosampler that provides additional sample capacity on a commercial protein sequencer. The autosampler attaches to a standard ABI Procise sequencer, enabling a single-sample cartridge to hold up to six separate samples. The autosampler is used in combination with faster Edman cycles and a rapid 12-min PTH separation to significantly increase the speed of automated protein sequencing. We also describe Edman chemistry modifications that improve coupling efficiency and prevent internal cleavage that can occur when samples are sequenced in the standard glass cartridges.
Pham
Victoria
V
Department of Protein Chemistry, Genentech, Inc., 1 DNA Way, South San Francisco, California 94080, USA.
Tropea
Jake
J
Wong
Suzy
S
Quach
James
J
Henzel
William J
WJ
D000818:Animals; D001331:Automation; D004867:Equipment Design; D004864:Equipment and Supplies; D006801:Humans; D007202:Indicators and Reagents; D020539:Sequence Analysis, Protein / Q000295:instrumentation* / Q000379:methods
D007202:Indicators and Reagents