Araçatuba virus: a vaccinialike virus associated with infection in humans and cattle.
We describe a vaccinialike virus, Araçatuba virus, associated with a cowpoxlike outbreak in a dairy herd and a related case of human infection. Diagnosis was based on virus growth characteristics, electron microscopy, and molecular biology techniques. Molecular characterization of the virus was done by using polymerase chain reaction amplification, cloning, and DNA sequencing of conserved orthopoxvirus genes such as the vaccinia growth factor (VGF), thymidine kinase (TK), and hemagglutinin. We used VGF-homologous and TK gene nucleotide sequences to construct a phylogenetic tree for comparison with other poxviruses. Gene sequences showed 99% homology with vaccinia virus genes and were clustered together with the isolated virus in the phylogenetic tree. Araçatuba virus is very similar to Cantagalo virus, showing the same signature deletion in the gene. Araçatuba virus could be a novel vaccinialike virus or could represent the spread of Cantagalo virus.
de Souza Trindade
Giliane
G
Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.
da Fonseca
Flávio Guimarães
FG
Marques
João Trindade
JT
Nogueira
Maurício Lacerda
ML
Mendes
Luiz Claudio Nogueira
LC
Borges
Alexandre Secorun
AS
Peiró
Juliana Regina
JR
Pituco
Edviges Maristela
EM
Bonjardim
Cláudio Antônio
CA
Ferreira
Paulo César Peregrino
PC
Kroon
Erna Geessien
EG
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D021821:Communicable Diseases, Emerging / Q000473:pathology; D005814:Genes, Viral / Q000235:genetics; D006801:Humans; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D018155:Orthopoxvirus / Q000235:genetics; D010802:Phylogeny; D016415:Sequence Alignment; D014615:Vaccinia / Q000473:pathology / Q000662:veterinary / Q000821:virology*; D014616:Vaccinia virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics; D014766:Viremia / Q000453:epidemiology
C088596:A-type inclusion protein, poxvirus; D014764:Viral Proteins; D015678:Viral Structural Proteins