title: Expression of B-lymphocyte-associated transcription factors in human T-cell neoplasms.
abstract: In this study we have investigated the expression of three B-cell-associated transcription factors in normal lymphoid tissue and in T-cell neoplasms (three cell lines, and more than 50 biopsy samples). Nuclear OCT-1 immunoreactivity was seen in normal B cells, in many extrafollicular T cells, and in a heterogeneous pattern (ranging in intensity from weak to moderate) in most T-cell neoplasms. OCT-2 immunostaining was primarily restricted in normal lymphoid tissue to B cells, and was absent from most T-cell neoplasms. In contrast, immunostaining for BOB-1/OCA-B--essentially restricted to B cells in normal lymphoid tissue, with the exception of activated T-lymphocytes--was seen in all of the T-cell lines tested and the majority of the tumor cells in all categories of T-cell lymphoma. Thus labeling for each of these three B-cell-associated transcription factors can be seen to varying degrees in T-cell neoplasms. However, the high frequency of BOB-1 expression in T-cell neoplasms, in contrast to its absence from resting peripheral T cells, suggests that its expression might be a prerequisite for neoplastic transformation, and prompts a search for the transcriptional target(s) of this factor in T cells.
authors.affiliation: Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Oxford, United Kingdom. teresa.marafioti@ndcls.ox.ac.uk
authors.forename: Teresa
authors.initials: T
authors.lastname: Marafioti
authors.forename: Stefano
authors.initials: S
authors.lastname: Ascani
authors.forename: Karen
authors.initials: K
authors.lastname: Pulford
authors.forename: Elena
authors.initials: E
authors.lastname: Sabattini
authors.forename: Milena
authors.initials: M
authors.lastname: Piccioli
authors.forename: Margaret
authors.initials: M
authors.lastname: Jones
authors.forename: Pier Luigi
authors.initials: PL
authors.lastname: Zinzani
authors.forename: Georges
authors.initials: G
authors.lastname: Delsol
authors.forename: David Y
authors.initials: DY
authors.lastname: Mason
authors.forename: Stefano A
authors.initials: SA
authors.lastname: Pileri
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D001706:Biopsy; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics; D051863:Host Cell Factor C1; D006801:Humans; D007150:Immunohistochemistry; D019169:Jurkat Cells; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; C497692:HCFC1 protein, human; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; C494165:POU2F1 protein, human; C058435:POU2F2 protein, human; D014157:Transcription Factors