title: Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells.
abstract: We have evaluated the feasibility of large-scale isolation of CD133+ progenitors from healthy mobilized adult donors for potential clinical use in autologous and allogeneic transplantation. A total of 11 healthy volunteer adult donors were mobilized with G-CSF. CD133+ stem cells were isolated from a single leukapheresis using the Clinimacs method. The median percentage of CD133 before positive selection was 0.75% (range 0.39-2.03%). After selection, the median purity and recovery was 94% (range 85.2-98.0%) and 69% (range 44-100%), respectively. The median log10 T-cell depletion obtained by CD133+ positive selection was 4.2 (range 3.8-4.7). The CD133+ progenitors were highly enriched in colony-forming units (CFU) and transplantation into NOD/SCID mice resulted in a high engraftment rate. Transplantation of sorted CD133+/CD34+ cells into NOD/SCID mice showed a higher engraftment compared to CD133-/CD34+ cells. Mobilized peripheral CD133+ stem cells can be purified in large scale for potential clinical use. The biological function of the cells is not impaired. The majority of the NOD/SCID repopulating cells are within the CD133+/CD34+ subpopulation. Therefore, clinical studies using purified CD133+ stem cells can be envisoned to further clarify the role of CD133+ stem cells in hematopoietic reconstitution after transplantation.
authors.affiliation: Division of Stem Cell, St Jude Children's Research Hospital, Memphis, TN 38105, USA.
authors.forename: P R
authors.initials: PR
authors.lastname: Gordon
authors.forename: T
authors.initials: T
authors.lastname: Leimig
authors.forename: A
authors.initials: A
authors.lastname: Babarin-Dorner
authors.forename: J
authors.initials: J
authors.lastname: Houston
authors.forename: M
authors.initials: M
authors.lastname: Holladay
authors.forename: I
authors.initials: I
authors.lastname: Mueller
authors.forename: T
authors.initials: T
authors.lastname: Geiger
authors.forename: R
authors.initials: R
authors.lastname: Handgretinger
mesh_terms: D000071916:AC133 Antigen; D000328:Adult; D000818:Animals; D015703:Antigens, CD / Q000097:blood; D002469:Cell Separation; D003114:Colony-Forming Units Assay; D017931:DNA Primers; D000069585:Filgrastim; D005434:Flow Cytometry; D006023:Glycoproteins / Q000097:blood; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D006801:Humans; D007937:Leukapheresis; D019520:Living Donors; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010455:Peptides / Q000097:blood; D016133:Polymerase Chain Reaction / Q000379:methods; D011994:Recombinant Proteins; D014183:Transplantation, Heterologous
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D017931:DNA Primers; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D000069585:Filgrastim