title: Microsatellite dinucleotide (T-G) repeat: a candidate DNA marker for breast metastasis.
abstract: A dinucleotide (T-G) repeat sequence was isolated by comparing DNA from metastatic lymph node and matched normal breast samples from a ductal mammary carcinoma patient using representational difference analysis (RDA) method. Our present study used this metastasis associated DNA sequence (MADS) as a diagnostic probe to screen five patient samples by slot blot method. A new approach to isolate single cells by microdissection, namely single cell microdissection (SCM) was developed to obtain homogeneous population of tumor cells (approximately 1000) from matched primary tumors and corresponding positive lymph nodes of five patients. We isolated DNA from these homogeneous tumor cells and used for the RDA and DNA slot blot experiments. The screening of patient samples showed loss of this MADS in the transition from primary to metastasis in four out of five cases (80%) suggesting its possible role in breast metastasis.
authors.affiliation: Metastasis Laboratory, Department of Radiation Oncology, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Mukherjee
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Bhupesh
authors.initials: B
authors.lastname: Parashar
authors.forename: Brij M
authors.initials: BM
authors.lastname: Sood
authors.forename: Panna S
authors.initials: PS
authors.lastname: Mahadevia
authors.forename: Harold P
authors.initials: HP
authors.lastname: Klinger
authors.forename: Bhadrasain
authors.initials: B
authors.lastname: Vikram
authors.forename: Mohanrao P
authors.initials: MP
authors.lastname: Achary
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D015139:Blotting, Southern; D001943:Breast Neoplasms / Q000235:genetics*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics*; D002469:Cell Separation / Q000379:methods; D004273:DNA, Neoplasm / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D005819:Genetic Markers