An immunohistochemical study on the neuropeptide-producing endocrine cells in the alimentary tract of wrinkled frog, Rana rugosa (Ranidae).
The regional distribution and relative frequency of neurohormonal peptides-producing endocrine cells were demonstrated in the alimentary tract of wrinkled frog, Rana rugosa, using eight types of specific antisera raised against mammalian regulatory peptides. The alimentary tract of frog was divided into six portions from esophagus to rectum. Most of the cells in the epithelial lining portion, between epithelial cells, were generally spherical or spindle shaped having long cytoplasmic process that was reached to the lumen (open-typed cell) while cells showing round shape (close-typed cell) were also found in the gastric, esophageal or intestinal glands occasionally. All of eight immunoreactive (IR) cells against serotonin, somatostatin, bovine Sp-1/chromogranin (BCG), gastrin, cholecystokinin (CCK)-8, bombesin, glucagon, and human pancreatic polypeptide (HPP) were observed in this study. Serotonin-IR cells were demonstrated throughout whole alimentary tract including esophagus and showed most predominant frequency in antrum. Somatostatin-IR cells were also detected throughout whole alimentary tract including esophagus and showed most predominant in pylorus and antrum. BCG-IR cells were restricted to stomach regions with relatively low frequencies. CCK-8-IR cells were observed from antrum to ileum and showed highest frequency in antrum. Gastrin-IR cells were restricted to antrum with low frequency and bombesin-IR cells were demonstrated from esophagus to antrum with various frequencies. Glucagon-IR cells were located throughout whole alimentary tract except for rectum and showed most predominant frequency at antrum. HPP-IR cells were detected from antrum to ileum with highest frequency in antrum. In conclusion, the regional distribution and relative frequency of these IR cells correspond well to the previous report in anuran species but somewhat peculiar patterns are also detected.
Ku
Sae-kwang
SK
Pharmacology and Toxicology Laboratory, Central Research Laboratories, Dong-Wha Pharmaceutical Industrial Co, Anyang 430-017, Republic of Korea.
Lee
Hyeung-sik
HS
Koh
Jae-kee
JK
Lee
Jae-hyun
JH
D000818:Animals; D000918:Antibody Specificity; D001839:Bombesin / Q000378:metabolism; D004064:Digestive System / Q000166:cytology / Q000378:metabolism*; D004702:Endocrine Glands / Q000166:cytology / Q000378:metabolism*; D005755:Gastrins / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D009163:Mycobacterium bovis / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism*; D010191:Pancreatic Polypeptide / Q000378:metabolism; D011898:Ranidae; D012701:Serotonin / Q000378:metabolism; D012844:Sincalide / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism; D014018:Tissue Distribution
D005755:Gastrins; D009479:Neuropeptides; D012701:Serotonin; D013004:Somatostatin; D010191:Pancreatic Polypeptide; D005934:Glucagon; D012844:Sincalide; D001839:Bombesin