[Therapeutic effect of high-density lipoprotein of human plasma on rat endotoxemia].
OBJECTIVE
To investigate the therapeutic effect of high-density lipoprotein (HDL) of human plasma on rat endotoxemia.


METHODS
Rats were divided into 2 groups randomly, the treated group received bolus intravenous endotoxin (500 EU/kg), then followed by a bolus HDL (75 mg/kg) intravenously as their blood pressure decreased obviously. The control group just received bolus intravenously endotoxin (500 EU/kg) without HDL. Blood pressure, survival time, the concentration of TNF and the levels of endotoxin in plasma were determined using radioimmunoassay and limulus lysate test.


RESULTS
The decrease of blood pressure was obviously attenuated (P < 0.01), the survival time was significantly increased (P < 0.01) and the concentration of plasma TNF was decreased (P < 0.05) in the treated group, compared with those in the control group. The levels of endotoxin were of no significant difference between the two groups (P > 0.05).


CONCLUSIONS
HDL of human plasma has obvious therapeutic effect on rat endotoxemia it could enhance the resistance of rats to endotoxemia, and the mechanism therein may be related to the inhibition of the release of TNF.
Huang
Ying
Y
Department of Pathophysiology, West China School of Predinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Gu
Ling
L
Wang
Shuren
S
Kang
Yan
Y
Wu
Shuhong
S
Cui
Wei
W
D000818:Animals; D019446:Endotoxemia / Q000628:therapy*; D006801:Humans; D008075:Lipoproteins, HDL / Q000627:therapeutic use*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D008075:Lipoproteins, HDL; D014409:Tumor Necrosis Factor-alpha