Crystallization and preliminary X-ray crystallographic studies of salt-tolerant glutaminase from Micrococcus luteus K-3.
Glutaminase from the marine bacterium Micrococcus luteus K-3 (Micrococcus glutaminase) is a salt-tolerant protein which shows equivalent activities both in the absence and the presence of 3 M sodium chloride and is distinct from halophilic proteins, which are inactivated in the absence of salt. To investigate the mechanisms of the salt-tolerant adaptation of Micrococcus glutaminase, the glutaminase and its major fragment containing about 80% of the protein were crystallized using the hanging-drop vapour-diffusion method. The glutaminase crystals belong to space group P622, with unit-cell parameters a = b = 111.4, c = 210.9 A, alpha = beta = 90, gamma = 120 degrees, and diffract to 2.6 A resolution. The fragment crystals belong to space group F222, with unit-cell parameters a = 115.7, b = 116.4, c = 144.9 A, alpha = beta = gamma = 90 degrees, and diffract to 2.4 A resolution. Data from selenomethionine (SeMet) substituted glutaminase crystals and from SeMet-substituted fragment crystals were collected to 2.6 and 2.4 A resolution, respectively. Structural analyses of the glutaminase and its fragment are currently being attempted using the multiwavelength anomalous diffraction (MAD) phasing method.
Chantawannakul
Panuwan
P
Venture Business Laboratory, Oita University, Oita 870-1192, Japan. mmorigu@oita-cc.cc.oita-u.ac.jp
Yoshimune
Kazuaki
K
Shirakihara
Yasuo
Y
Shiratori
Aya
A
Wakayama
Mamoru
M
Moriguchi
Mitsuaki
M
D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D005972:Glutaminase / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D016982:Micrococcus luteus / Q000201:enzymology*; D008970:Molecular Weight
D005972:Glutaminase; C477762:salt-tolerant glutaminase