Short-term androgen deprivation does not alter CaR and VDR mRNA expression in duodenal mucosa in male rats.
Androgen deprivation is associated with decline in intestinal calcium absorption. The effect of androgen on CaR and VDR intestinal mucosa has not yet been studied. Calcium homeostasis, a real bone mineral density (aBMD, dual energy X-ray absorptiometry) and expression of CaR and VDR mRNA in duodenal mucosa of orchidectomized (ORX) and sham operated (Sham) adult Sprague Dawley rats at 4 week have been studied. There was no significant difference in serum calcium, alkaline phosphatase, calcidiol and calcitriol levels between both the groups. Serum testosterone (T) (ng/dl) and inorganic phosphorous (iP) (mg/dl) levels were significantly lower in ORX rats. As compared to sham rats, ORX rats had significant decline in in-vitro aBMD at proximal, middle and distal tibia, proximal, mid and distal femur and femoral neck (P < 0.05). Northern blot analysis revealed no significant alteration in the CaR and VDR mRNA expression in duodenal mucosa in ORX rats. CaR and VDR mRNA expression in duodenal mucosa is therefore, not affected by physiological concentrations of testosterone in rats.
Tiwari
S
S
Department of Medical Endocrinology, Center of Endocrine Sciences, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow 226014, India.
Gupta
S K
SK
Mehrotra
M
M
Agarwal
G
G
Awasthi
P K
PK
Godbole
M M
MM
D000728:Androgens / Q000172:deficiency* / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D002135:Calcium-Binding Proteins / Q000235:genetics*; D004386:Duodenum / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D009919:Orchiectomy; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018167:Receptors, Calcitriol / Q000235:genetics*
D000728:Androgens; D002135:Calcium-Binding Proteins; D012333:RNA, Messenger; D018167:Receptors, Calcitriol