Medium pH-dependent redistribution of the urease of Helicobacter pylori.
Helicobacter pylori is an aetiological agent of gastric disease. Although the role of urease in gastric colonization of H. pylori has been shown, it remains unclear as to where urease is located in this bacterial cell. The purpose of this study was to define the urease-associated apparatus in the H. pylori cytoplasm. H. pylori was incubated at both a neutral and an acidic pH in the presence or absence of urea and examined by double indirect immunoelectron microscopy. The density of gold particles for UreA was greatest in the inner portion of the wild-type H. pylori cytoplasm at neutral pH but was greatest in the outer portion at acidic pH. This difference was independent of the presence of urea and was not observed in the ureI-deletion mutant. Also, the eccentric shift of urease in acidic pH was not observed in UreI. After a 2 day incubation period at acidic pH, it was observed that the urease gold particles in H. pylori assembled and were associated with UreI gold particles. Urease immunoreactivity shifted from the inner to the outer portion of H. pylori as a result of an extracellular decrease in pH. This shift was urea-independent and UreI-dependent, suggesting an additional role of UreI in urease-dependent acid resistance. This is the first report of the intracellular transport of molecules in bacteria in response to changes in the extracellular environment.
Hong
Wu
W
Department of Microbiology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka 569-8686, Japan 2Department of Nursing, Kobe Tokiwa College, Ohtani-cho 2-6-2, Nagata-ku, Kobe, Hyogo 653-0838, Japan 3VA Greater Los Angeles Healthcare System and Department of Physiology and Medicine, University of California at Los Angeles, CA, USA.
Sano
Kouichi
K
Department of Microbiology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka 569-8686, Japan 2Department of Nursing, Kobe Tokiwa College, Ohtani-cho 2-6-2, Nagata-ku, Kobe, Hyogo 653-0838, Japan 3VA Greater Los Angeles Healthcare System and Department of Physiology and Medicine, University of California at Los Angeles, CA, USA.
Morimatsu
Shinichi
S
Department of Microbiology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka 569-8686, Japan 2Department of Nursing, Kobe Tokiwa College, Ohtani-cho 2-6-2, Nagata-ku, Kobe, Hyogo 653-0838, Japan 3VA Greater Los Angeles Healthcare System and Department of Physiology and Medicine, University of California at Los Angeles, CA, USA.
Scott
David R
DR
Department of Microbiology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka 569-8686, Japan 2Department of Nursing, Kobe Tokiwa College, Ohtani-cho 2-6-2, Nagata-ku, Kobe, Hyogo 653-0838, Japan 3VA Greater Los Angeles Healthcare System and Department of Physiology and Medicine, University of California at Los Angeles, CA, USA.
Weeks
David L
DL
Department of Microbiology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka 569-8686, Japan 2Department of Nursing, Kobe Tokiwa College, Ohtani-cho 2-6-2, Nagata-ku, Kobe, Hyogo 653-0838, Japan 3VA Greater Los Angeles Healthcare System and Department of Physiology and Medicine, University of California at Los Angeles, CA, USA.
Sachs
George
G
Department of Microbiology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka 569-8686, Japan 2Department of Nursing, Kobe Tokiwa College, Ohtani-cho 2-6-2, Nagata-ku, Kobe, Hyogo 653-0838, Japan 3VA Greater Los Angeles Healthcare System and Department of Physiology and Medicine, University of California at Los Angeles, CA, USA.
Goto
Toshiyuki
T
Department of Microbiology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka 569-8686, Japan 2Department of Nursing, Kobe Tokiwa College, Ohtani-cho 2-6-2, Nagata-ku, Kobe, Hyogo 653-0838, Japan 3VA Greater Los Angeles Healthcare System and Department of Physiology and Medicine, University of California at Los Angeles, CA, USA.
Mohan
Sharad
S
Department of Microbiology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka 569-8686, Japan 2Department of Nursing, Kobe Tokiwa College, Ohtani-cho 2-6-2, Nagata-ku, Kobe, Hyogo 653-0838, Japan 3VA Greater Los Angeles Healthcare System and Department of Physiology and Medicine, University of California at Los Angeles, CA, USA.
Harada
Fumiue
F
Department of Microbiology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka 569-8686, Japan 2Department of Nursing, Kobe Tokiwa College, Ohtani-cho 2-6-2, Nagata-ku, Kobe, Hyogo 653-0838, Japan 3VA Greater Los Angeles Healthcare System and Department of Physiology and Medicine, University of California at Los Angeles, CA, USA.
Nakajima
Norihito
N
Department of Microbiology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka 569-8686, Japan 2Department of Nursing, Kobe Tokiwa College, Ohtani-cho 2-6-2, Nagata-ku, Kobe, Hyogo 653-0838, Japan 3VA Greater Los Angeles Healthcare System and Department of Physiology and Medicine, University of California at Los Angeles, CA, USA.
Nakano
Takashi
T
Department of Microbiology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka 569-8686, Japan 2Department of Nursing, Kobe Tokiwa College, Ohtani-cho 2-6-2, Nagata-ku, Kobe, Hyogo 653-0838, Japan 3VA Greater Los Angeles Healthcare System and Department of Physiology and Medicine, University of California at Los Angeles, CA, USA.
D001426:Bacterial Proteins / Q000235:genetics; D003593:Cytoplasm / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D017353:Gene Deletion; D005798:Genes, Bacterial / Q000235:genetics; D016480:Helicobacter pylori / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D026901:Membrane Transport Proteins; D014508:Urea / Q000378:metabolism / Q000494:pharmacology; D014510:Urease / Q000378:metabolism*
D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; C114527:UreI protein, Helicobacter pylori; D014508:Urea; D014510:Urease