Nuclear localization of phosphorylated c-Myc protein in human tumor cells.
Using immunocytochemical techniques at light and electron microscopy, we analysed the distribution of phosphorylated c-Myc in actively proliferating human HeLa cells. The distribution pattern of c-Myc was also compared with those of other ribonucleoprotein (RNP)-containing components (PANA, hnRNP-core proteins, fibrillarin) or RNP-associated nuclear proteins (SC-35 splicing factor). Our results provide the first evidence that phosphorylated c-Myc accumulates in the nucleus of tumor cells, where it colocalizes with fibrillarin, both in the nucleolus and in extranucleolar structures.
Soldani
C
C
Bottone
M G
MG
Biggiogera
M
M
Alpini
C
C
Scovassi
A I
AI
Martin
T
T
Pellicciari
C
C
D002466:Cell Nucleolus / Q000378:metabolism / Q000648:ultrastructure; D002467:Cell Nucleus / Q000737:chemistry* / Q000648:ultrastructure*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D006367:HeLa Cells; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000378:metabolism; D006801:Humans; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology*; D010766:Phosphorylation; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis*
D002868:Chromosomal Proteins, Non-Histone; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D016271:Proto-Oncogene Proteins c-myc; C047458:fibrillarin