The ankyrin repeat containing SOCS box protein 5: a novel protein associated with arteriogenesis.
Arteriogenesis, the growth of pre-existing collateral arteries, can be induced in rabbit by occlusion of the femoral artery. In order to identify and characterize genes differentially expressed during the early phase of arteriogenesis, cDNA of collateral arteries 24h after femoral ligation or sham operation was subjected to suppression subtractive hybridization. We identified the ankyrin repeat containing SOCS box protein 5 (asb5) and cloned the rabbit full-length cDNA. Asb5 was demonstrated to be a single-copy gene. We localized the asb5 protein in vivo in endothelial and smooth muscle cells of collateral arteries as well as in satellite cells. Asb5 was significantly upregulated in growing collateral arteries on mRNA and protein level. The infusion of doxorubicin in rabbit led to a significant decrease of the asb5 mRNA. In summary, our data show that asb5 is a novel protein implicated in the initiation of arteriogenesis.
Boengler
Kerstin
K
Department of Experimental Cardiology, Max-Planck-Institute for Physiological and Clinical Research, Benekestr. 2, D-61231 Bad Nauheim, Germany. k.boengler@kerckhoff.mpg.de
Pipp
Frederic
F
Fernandez
Borja
B
Richter
Alexandra
A
Schaper
Wolfgang
W
Deindl
Elisabeth
E
D000595:Amino Acid Sequence; D000818:Animals; D017089:Ankyrin Repeat; D001158:Arteries / Q000254:growth & development* / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation
D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger