title: Influences of different stress models on the antioxidant status and lipid peroxidation in rat erythrocytes.
abstract: The aim of this study was to investigate the influences of different stress models on the antioxidant status and lipid peroxidation (LPO) in erythrocytes of rats. Swiss-Albino female rats (3 months old) were used in this study. Rats were randomly divided into the following four groups; control group (C), cold stress group (CS), immobilization stress group (IS) and cold + immobilization stress group (CS + IS). Control group was kept in an animal laboratory (22 +/- 2 degrees C). Rats in CS group were placed in cold room (5 degrees C) for 15min/day for 15 days. Rats in IS group were immobilized for 180 min/day for 15 days. Rats in CS + IS group were exposed to both cold and immobilization stresses for 15 days. At the end of experimental periods, the activities of glucose-6-phosphate dehydrogenase (G-6-PD), Cu,Zn-superoxide dismutase (Cu,Zn-SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), and concentration of reduced glutathione (GSH) were measured. LPO was determined by measuring the contents of thiobarbituric acid-reactive substances (TBARS). Cu,Zn-SOD activity and TBARS concentration were increased after cold and immobilization stresses, but CAT and GSH-Px activities and GSH levels were decreased. Immobilization stress decreased the activity of G-6-PD. The activities of G-6-PD, CAT and GSH-Px, and the level of GSH were lower in CS + IS group than in the control group. Cu,Zn-SOD activity and TBARS levels were increased in CS + IS group when compared with the control group. From these findings, three stress models are thought to cause oxidative stress.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Akdeniz University, 07070 Arapsuyu, Antalya, Turkey. gumuslu@hipokrat.med.akdeniz.edu.tr
authors.forename: Saadet
authors.initials: S
authors.lastname: Gümüşlü
authors.forename: Süreyya Bilmen
authors.initials: SB
authors.lastname: Sarikçioğlu
authors.forename: Emel
authors.initials: E
authors.lastname: Sahin
authors.forename: Piraye
authors.initials: P
authors.lastname: Yargiçoğlu
authors.forename: Aysel
authors.initials: A
authors.lastname: Ağar
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D051381:Rats; D017392:Thiobarbituric Acid Reactive Substances; D013997:Time Factors
chemical_list: D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D005978:Glutathione