title: Introgression of 1Dx5+1Dy10 into tritordeum.
abstract: The uses of hexaploid tritordeum as a crop for human consumption require improvement of its bread-making quality. For this purpose chromosome 1D of bread wheat with the Glu-D1 allele encoding for high-molecular-weight glutenin subunits Dx5+Dy10 was introgressed into tritordeum. Different primary tritordeums were crossed with wheats carrying subunits Dx5+Dy10. The hybrids were backcrossed to tritordeum and seeds for the next backcross (or selfing) were selected for the presence of chromosome 1D using SDS-PAGE. Forty two chromosome plants carrying subunits Dx5+Dy10 were obtained after two backcrosses and selfing. Chromosome characterization of these plants using fluorescence in situ hybridisation (FISH) proved that either chromosome substitution 1H(ch)/1D or 1A/1D had been obtained. A homozygous plant with a translocation of the entire 1DL arm to 1H(ch)S was also obtained. The complete chromosome substitution lines have better agronomic characteristics than the lines with translocations.
authors.affiliation: Departamento de Agronomía y Mejora Genética Vegetal, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Apdo. 4084, E-14080 Córdoba, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Ballesteros
authors.forename: J B
authors.initials: JB
authors.lastname: Alvarez
authors.forename: M J
authors.initials: MJ
authors.lastname: Giménez
authors.forename: M C
authors.initials: MC
authors.lastname: Ramírez
authors.forename: A
authors.initials: A
authors.lastname: Cabrera
authors.forename: A
authors.initials: A
authors.lastname: Martín
mesh_terms: D000483:Alleles; D002678:Chimera; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004591:Electrophoresis, Polyacrylamide Gel; D005983:Glutens / Q000031:analogs & derivatives* / Q000235:genetics; D006720:Homozygote; D001467:Hordeum / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*
chemical_list: D005983:Glutens; C037302:glutenin