Presence of endometrial epithelial cells in the peritoneal cavity and the mesothelial inflammatory response.
OBJECTIVE
To determine the contribution of endometrial cells in the development of endometriosis. Specifically the response of the mesothelium to endometrial cells in the production of monocyte chemotactic protein-1 (MCP-1), interleukin-6 (IL-6), and IL-8 was studied.


METHODS
In vitro study.


METHODS
University Research Laboratory.


METHODS
None.


METHODS
None.


METHODS
Cellular MCP-1, IL-6 secretion and MCP-1, and IL-6 and IL-8 messenger RNA expression were evaluated by ELISA and reverse transcription-polymerase chain reaction (RT-PCR) assay.


RESULTS
The mesothelial cells produced more MCP-1 and IL-6 than endometrial epithelial and stromal cells. Mesothelial cells cultured in the presence of endometrial epithelial cells produced even greater levels of MCP-1 and IL-6 than those cultured in the presence of stromal cells or cultured alone. The MCP-1, IL-6, and IL-8 mRNA expression also increased when mesothelial cells were co-cultured with endometrial epithelial cells.


CONCLUSIONS
The results suggest that endometrial epithelial cells may be important in evoking the inflammatory reaction in the peritoneal cavity during retrograde menstruation and that mesothelial cells may play an important role in the chemotaxis of monocytes and in the inflammatory process during the development of endometriosis.
Song
Mingqing
M
Emory Center for Advanced Research on Women's Health, Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
Karabina
Sonia A
SA
Kavtaradze
Nino
N
Murphy
Ana A
AA
Parthasarathy
Sampath
S
D018932:Chemokine CCL2 / Q000096:biosynthesis / Q000235:genetics; D018920:Coculture Techniques; D004715:Endometriosis / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics; D010537:Peritoneum / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018932:Chemokine CCL2; D015850:Interleukin-6; D016209:Interleukin-8; D012313:RNA