Crystallization and preliminary crystallographic studies of mung bean cytokinin-specific binding protein.
Cytokinins, or plant growth hormones, bind with very high affinity to cytokinin-specific binding proteins (CSBPs). Recombinant mung bean CSBP has been overexpressed in Escherichia coli and crystallized in complex with zeatin, a natural plant growth hormone. The crystals belong to the hexagonal system, space group P6(2) or P6(4), with unit-cell parameters a = 113.62, c = 86.85 A, contain two to five copies of the protein in the asymmetric unit and diffract X-rays to 1.25 A resolution.
Bujacz
Grzegorz D
GD
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland, and Institute of Technical Biochemistry, Technical University of Łódź, Poland.
Pasternak
Oliwia
O
Fujimoto
Yasuyuki
Y
Hashimoto
Yuichi
Y
Sikorski
Michal M
MM
Jaskolski
Mariusz
M
D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry*; D011994:Recombinant Proteins; D015026:Zeatin / Q000737:chemistry
D002352:Carrier Proteins; D011994:Recombinant Proteins; D015026:Zeatin