Bsp1p/Ypr171p is an adapter that directly links some synaptojanin family members to the cortical actin cytoskeleton in yeast.
In this study we identified a novel protein, Bsp1p, that interacts directly with two yeast synaptojanins, Sjl2p and Sjl3p, but not with Sjl1p. The interaction takes place via the Sac1/polyphosphoinositide phosphatase domain, whose conserved C-terminal region is important for binding. Subcellular localization and genetic interactions revealed a function of Bsp1p in the cortical actin cytoskeleton. A fraction of Bsp1p was found to be membrane-associated. Studies with mutants of phosphatidylinositol 4-kinase, PIK1, suggested that the interaction with membranes is facilitated by phosphoinositides. We propose that Bsp1p is an adapter that links Sjl2p and Sjl3p to the cortical actin cytoskeleton.
Wicky
Sidonie
S
Institute for Biochemistry, University of Stuttgart, Pfaffenwaldring 55, Germany.
Frischmuth
Sabine
S
Singer-Krüger
Birgit
B
D000199:Actins / Q000378:metabolism*; D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003599:Cytoskeleton / Q000378:metabolism*; D017931:DNA Primers; D005838:Genotype; D007700:Kinetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010716:Phosphatidylinositols / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism
D000199:Actins; D033942:Adaptor Proteins, Vesicular Transport; C473114:Bsp1 protein, S cerevisiae; D017931:DNA Primers; D010716:Phosphatidylinositols; D029701:Saccharomyces cerevisiae Proteins; C110551:INP51 protein, S cerevisiae; C110552:INP52 protein, S cerevisiae; D010744:Phosphoric Monoester Hydrolases