Non-aqueous capillary electrophoresis of the positional isomers of a sulfated monosaccharide.
A non-aqueous capillary electrophoresis (NACE) method coupled to indirect absorbance detection has been developed for the separation of the three positional isomers of monosulfated fucose. The optimized electrolyte was composed of 12 mM ethanolamine, 2 mM trimesic acid buffer in a methanol-ethanol (1:1, v/v) mixture. As the retained electrolyte entails no separating agent other than the pH buffer, the NACE separation of the positional isomers has been ascribed mainly to selective ion-pairing with the electrolyte counter-ion and the possibility of a selective solvation effect in the alcohol mixture. In the absence of pure isomeric standards, peak identification was completed by MS and NMR spectroscopy and selective enzymatic desulfation. This method should be of interest for the structure elucidation of monosulfated fucose-based polysaccharides and for the screening of sulfoesterase of unknown activity.
Descroix
Stéphanie
S
Laboratoire d'Electrochimie et Chimie Analytique, UMR CNRS 7575, ENSCP, 11 Rue Pierre et Marie Curie, 75231 Paris 05, France.
Varenne
Anne
A
Goasdoue
Nicole
N
Abian
Joaquin
J
Carrascal
Montse
M
Daniel
Régis
R
Gareil
Pierre
P
D019075:Electrophoresis, Capillary / Q000379:methods*; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009005:Monosaccharides / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry*
D009005:Monosaccharides; D013431:Sulfates