[Effects of wild-type p53 gene transfection on the growth and cisplatin sensitivity of cervical cancer cell line HeLa].
OBJECTIVE
To investigate the effects of human wild-type p53 gene transfection on the growth and chemosensitivity of human cervical carcinoma.


METHODS
Recombinant eukaryotic expression vector pCB6.p53 containing human wild-type p53 cDNA was introduced by lipofectamine transfection regent into HeLa cell line. The expression of p53 was detected by immunohistochemistry. Cell proliferation was measured by methyl thiazolyl tetrazolium after the treatment of cisplatin.


RESULTS
Expression of p53 was detected by immunohistochemistry. The growth rate of p53-transfected HeLa decreased. Wild-type p53-positive HeLa was more sensitive to cisplatin, compared with the control cell lines.


CONCLUSIONS
The exogenous wild-type p53 expression not only induced great suppression of cell growth but also increased chemosensitivity of human cervical carcinoma.
Wang
Xiaoli
X
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Peng
Zhilan
Z
Wang
He
H
D000970:Antineoplastic Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D016158:Genes, p53 / Q000235:genetics*; D006367:HeLa Cells / Q000166:cytology; D006801:Humans; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
D000970:Antineoplastic Agents; D016159:Tumor Suppressor Protein p53; D002945:Cisplatin