Effect of capsaicin on ion transport in the caecum of rabbits.
Effect of capsaicin, a stimulator of C-fibres, on ion transport in the caecum of rabbits was studied using electrophysiological methods, designed to evaluate ionic currents occurring in epithelial tissues. The experiments consisted in measuring transepithelial electrical potential difference (dPD) of an isolated fragment of rabbit's caecum, placed in a Ussing apparatus. The ion transport was modified through incubation in Ringer solution, supplemented with amiloride, bumetanide, and capsaicin. Capsaicin was also administered with peristalting pump. The experiments demonstrated that the inhibition of sodium ions transport caused by incubation with amiloride and incubation with capsaicin slowed down mechanical reaction to electrical potential difference. On the other hand, immediately after the administration, the capsaicin effect on C-fibres modified electrophysiological reaction of the caecum to mechanical stimulation. Physiological and pharmacological experiments reveal that a component dependent on activation of C-fibres contributes to the reaction of ion transport activation following mechanical stimulation.
Kosik-Bogacka
Danuta
D
Department of Biology and Medical Parasitology, Pomeranian Medical University Powstańców Wlkp. 72, PL 70-111 Szczecin, Poland. kodan@sci.pam.szczecin.pl
Banach
Bolesław
B
Tyrakowski
Tomasz
T
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002034:Bumetanide / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology*; D002432:Cecum / Q000187:drug effects* / Q000502:physiology; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000502:physiology; D004594:Electrophysiology; D005260:Female; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects / Q000294:innervation / Q000502:physiology; D017136:Ion Transport / Q000187:drug effects* / Q000502:physiology; D008297:Male; D036421:Nerve Fibers, Unmyelinated / Q000187:drug effects / Q000502:physiology; D010812:Physical Stimulation; D011817:Rabbits; D026941:Sodium Channel Blockers / Q000494:pharmacology; D013997:Time Factors
D018118:Chloride Channels; D026941:Sodium Channel Blockers; D002034:Bumetanide; D000584:Amiloride; D002211:Capsaicin