title: Efficient protocol for in vitro direct plant regeneration in chickpea Cicer arietinum L.
abstract: An efficient plant regeneration system was developed for two important Indian chickpea cultivars, C-235 and HC-1. Immature cotyledons (7-8 mm) directly formed shoots without an intervening callus phase on MS medium containing B5 vitamins, BAP (2.0 mg/l), IBA (0.125 mg/l), AgNO3 (1.69 mg/l) and phytagel (2.5 g/l). The regenerated shoots had normal morphology and were successfully rooted in half strength MS medium under partial dark conditions. Regenerated plants were transferred to potted soil. However, the survival rate of pot house transferred plants was 17.6 per cent.
authors.affiliation: Department of Biotechnology and Molecular Biology, CCS Haryana Agricultural University, Hisar 125 004, India.
authors.forename: Praveen
authors.initials: P
authors.lastname: Batra
authors.forename: Neelam R
authors.initials: NR
authors.lastname: Yadav
authors.forename: Anil
authors.initials: A
authors.lastname: Sindhu
authors.forename: R C
authors.initials: RC
authors.lastname: Yadav
authors.forename: V K
authors.initials: VK
authors.lastname: Chowdhury
authors.forename: J B
authors.initials: JB
authors.lastname: Chowdhury
mesh_terms: D029863:Cicer / Q000254:growth & development / Q000502:physiology*; D066298:In Vitro Techniques