Selective production of fungal beauveriolide I or III by fermentation in amino acid-supplemented media.
Beauveriolides I and III, cyclic depsipeptides composed of L-Phe, L-Ala, D-Leu and (3S,4S)-3-hydroxy-4-methyloctanoic acid, and L-Phe, L-Ala, L-allo-Ile and (3S,4S)-3-hydroxy-4-methyloctanoic acid, respectively, were previously isolated from the culture broth of fungal Beauveria sp. FO-6979 as inhibitors of macrophage foam cell formation. To improve the production of these compounds by fermentation, the culture conditions were studied. The production of both beauveriolides was increased five to ten folds by fermentation in the culture media containing tryptone. Further study revealed that addition of L-Leu/L-Ile, but not D-Leu/D-allo-Ile, to the culture medium yielded a high and selective production of beauveriolide I or III. As a result, regardless of their separation difficulty due to the similar physico-chemical properties, a large amount of beauveriolide I or III was prepared from the culture broth obtained from L-Leu- or L-Ile-supplemented fermentation, respectively, by one step purification using silica gel column chromatography.
Namatame
Ichiji
I
Kitasato Institute for Life Sciences, Graduate School of Infection Control Sciences, Kitasato University, Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Matsuda
Daisuke
D
Tomoda
Hiroshi
H
Yamaguchi
Yuichi
Y
Masuma
Rokuro
R
Kobayashi
Susumu
S
Omura
Satoshi
S
D000596:Amino Acids / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D047630:Depsipeptides; D005285:Fermentation; D006999:Hypocreales / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods; D010456:Peptides, Cyclic / Q000302:isolation & purification / Q000378:metabolism*
D000596:Amino Acids; D003470:Culture Media; D047630:Depsipeptides; D010456:Peptides, Cyclic; C013833:beauverolides