title: [Subcellular localization of basic Krüppel-like factor].
abstract: To understand the function of basic Krüppel-like factor (BKLF), it was confirmed by direct fluorescence and indirect fluorescence observation that hBKLF was localized in nucleus, and distributed throughout nucleoplasm in a speckled pattern, except the nucleoli. This pattern is similar to many but not all transcription factors. To clarify the specific sequence responsible for its nuclear localization, a series of deletion mutants of GFP/hBKLF were constructed. By observing their subcellular localization, it was found that the three zinc fingers of hBKLF and the N-terminal aside from the fingers all served as nuclear localization signals (NLS); the sub-NLS of hBKLF was located in the N-terminus, including the CtBP-binding motif and the proline rich domain. These results provided a basis for further clarifying the function of BKLF.
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Ma
authors.forename: Mang-Ju
authors.initials: MJ
authors.lastname: Wang
authors.forename: Xiang-Hu
authors.initials: XH
authors.lastname: Qu
authors.forename: Gui-Chun
authors.initials: GC
authors.lastname: Xing
authors.forename: Yun-Ping
authors.initials: YP
authors.lastname: Zhu
authors.forename: Fu-Chu
authors.initials: FC
authors.lastname: He
mesh_terms: D000818:Animals; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; C501168:KLF3 protein, human; D051741:Kruppel-Like Transcription Factors; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins