Protease-activated receptor-2-mediated contraction in the rat urinary bladder: the role of urinary bladder mucosa.
The role of protease-activated receptor-2 (PAR-2) in the regulation of the rat urinary bladder contractility was investigated. Both trypsin and PAR-2 activating peptide (SLIGRL-NH(2)) produced a concentration-dependent contractile response in the urinary bladder preparations. These contractions were abolished by removal of the urinary bladder mucosa and were significantly reduced by indomethacin (10 microM). These results suggest that activation of PAR-2 stimulates release of prostaglandins from mucosal layer and thereby contracts rat urinary bladder smooth muscles.
Nakahara
Tsutomu
T
Department of Molecular Pharmacology, Kitasato University School of Pharmaceutical Sciences, 5-9-1 Shirokane, Minato-ku, 108-8641, Tokyo, Japan. nakaharat@pharm.kitasato-u.ac.jp
Kubota
Yuko
Y
Mitani
Akiko
A
Maruko
Takeshi
T
Sakamoto
Kenji
K
Ishii
Kunio
K
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D009842:Oligopeptides / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D044464:Receptor, PAR-2 / Q000187:drug effects / Q000502:physiology*; D014357:Trypsin / Q000494:pharmacology; D001743:Urinary Bladder / Q000187:drug effects* / Q000502:physiology; D019459:Urothelium / Q000187:drug effects / Q000502:physiology
D009842:Oligopeptides; D044464:Receptor, PAR-2; C112356:seryl-leucyl-isoleucyl-glycyl--arginyl-leucinamide; D014357:Trypsin; D007213:Indomethacin