[Growth and osteogenesis characteristics of cultured rat marrow stromal cells under bone induction condition].
OBJECTIVE
To investigate the growth and osteogenesis characteristics of cultured rat marrow stromal cells(rMSCs) under bone induction condition.


METHODS
rMSCs were isolated from adult rat using density gradient separation method. The rMSCs attachment formed soon after seeding and grew into colonies with the appearance of fibroblastic cells. The osteogenic induction compound of Dex(10(-8) mol/L), beta-GP(10 mmol/L) and AA(50 micrograms/ml) was added to different passaged rMSCs and the proliferation and osteogenic differentiation of them was observed.


RESULTS
The induction compound had strong effect on promoting proliferation, especially on that of further passaged rMSCs, and its stimulation effect on osteogenesis was also well proved with the expression of alkaline phosphatase (ALP) rising after one-week induction. Further subculturing from P1 to P6 led to the increase of proliferation activity of rMSCs. After three-week induction, mineral deposits appeared in the culture.


CONCLUSIONS
The lower expression of ALP in P1 rMSCs and the increase of it in further passages or under induction condition suggest the rMSCs in our culture system are mainly undifferentiated osteoprogenitors.
Han
J
J
Biomedical Engineering Research Unit, WCUMS, Chengdu 610041, China.
Chen
R
R
Chen
H
H
Deng
L
L
Tang
F
F
Tian
W
W
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001846:Bone Development; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D005994:Glycerophosphates / Q000494:pharmacology; D010012:Osteogenesis; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology* / Q000201:enzymology
D005994:Glycerophosphates; D003907:Dexamethasone; D000469:Alkaline Phosphatase; D001205:Ascorbic Acid; C031463:beta-glycerophosphoric acid