Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR.
In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.
Heller
Loree C
LC
Esoteric Testing, Department of Pathology, Tampa General Hospital, PO Box 1289, Tampa, FL 33101, USA. lheller@tgh.org
Davis
Carisa R
CR
Peak
K Kealy
KK
Wingfield
David
D
Cannons
Andrew C
AC
Amuso
Philip T
PT
Cattani
Jacqueline
J
D000818:Animals; D001939:Bread / Q000382:microbiology; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005506:Food Contamination; D008461:Meat Products / Q000382:microbiology; D011933:Reagent Kits, Diagnostic; D022621:Shiga Toxin / Q000096:biosynthesis / Q000235:genetics*; D014675:Vegetables / Q000382:microbiology
D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic; D022621:Shiga Toxin