Determination of proteins with Fast Red VR by a corrected resonance light-scattering technique.
A simple corrected resonance light-scattering (CRLS) technique was established to correct for any distortion of the resonance light scattering (RLS) spectra resulting from molecular absorption. By using an absorption cell holder to change the propagation direction of the incident light beam of a common spectrofluorometer, the molecular absorption was directly measured through a spectrofluorometer. With measurements of the CRLS signals of the interaction of Fast Red VR (FRV) and proteins, we proved that the present correction for the RLS spectra in terms of the molecular absorption of excitation and scattering radiation can improve the detection sensitivity by about two fold.
Yang
Chuan Xiao
CX
Institute of Environmental Chemistry, College of Chemistry and Chemical Engineering, Southwest Normal University, Chongqing 400715, The People's Republic of China.
Li
Yuan Fang
YF
Huang
Cheng Zhi
CZ
D004396:Coloring Agents; D004867:Equipment Design; D008027:Light; D011506:Proteins / Q000032:analysis*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods / Q000592:standards
D004396:Coloring Agents; D011506:Proteins