title: Ralstonia basilensis M91-3, a denitrifying soil bacterium capable of using s-triazines as nitrogen sources.
abstract: The purpose of this study was to characterize the phylogenetic and phenotypic traits of M91-3, a soil bacterium capable of mineralizing atrazine (2-chloro-4-N-isopropyl-6-N-ethyl-s-triazine). The isolate was identified as Ralstonia basilensis based on 99.5% homology of the 16S rRNA sequence and various chemotaxonomic data. The isolate used atrazine as the sole source of energy, carbon, and nitrogen. It could also use several other s-triazines as nitrogen sources. Ralstonia basilensis M91-3 was capable of denitrification, which was confirmed by gas chromatographic analysis of nitrous oxide under acetylene blockage conditions.
authors.affiliation: Department of Microbiology, Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA.
authors.forename: David M
authors.initials: DM
authors.lastname: Stamper
authors.forename: Mark
authors.initials: M
authors.lastname: Radosevich
authors.forename: Kevin B
authors.initials: KB
authors.lastname: Hallberg
authors.forename: Samuel J
authors.initials: SJ
authors.lastname: Traina
authors.forename: Olli H
authors.initials: OH
authors.lastname: Tuovinen
mesh_terms: D001483:Base Sequence; D020562:Betaproteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016208:Databases, Factual; D005227:Fatty Acids / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D014227:Triazines / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014227:Triazines; D009584:Nitrogen