Plasmid R16 ArdA protein preferentially targets restriction activity of the type I restriction-modification system EcoKI.
The ArdA antirestriction protein of the IncB plasmid R16 selectively inhibited the restriction activity of EcoKI, leaving significant levels of modification activity under conditions in which restriction was almost completely prevented. The results are consistent with the hypothesis that ArdA functions in bacterial conjugation to allow an unmodified plasmid to evade restriction in the recipient bacterium and yet acquire cognate modification.
Thomas
Angela T
AT
Department of Genetics, University of Leicester, Leicester LE1 7RH, United Kingdom.
Brammar
William J
WJ
Wilkins
Brian M
BM
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D010582:Bacteriophage lambda / Q000472:pathogenicity / Q000502:physiology; D004262:DNA Restriction Enzymes / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000821:virology; D029968:Escherichia coli Proteins; D010957:Plasmids / Q000235:genetics*; D012097:Repressor Proteins
C096738:ArdA protein, E coli; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D012097:Repressor Proteins; D004262:DNA Restriction Enzymes; C089629:endodeoxyribonuclease EcoKI