Role of esterases and monooxygenase in the deltamethrin resistance in Anopheles stephensi Giles (1908), at Mysore.
Field collected An. stephensi larvae were colonized in the laboratory for 15 generations and acclimatized. An isofemale line was raised from this colony and the larvae were subjected to continuous deltamethrin selection pressure. LC50 and LC90 values were calculated at every generation. The values indicated that at the end of seventh generation the larvae have developed 87 fold tolerance in terms of LC50 value compared with the first generation. The reason for this kind of resistance was analyzed on the basis of differential activity of A-esterase, B-esterase, glutathione s-transferase (GST) and glucose 6-phosphate dehydrogenase (G6PD). A significant correlation (P < 0.05) was observed with B-esterase and G6PD activity with the rise in the LC50 and LC90 values. However no significant rise were observed in the other enzymes tested such as A-esterase and GST. The isozyme analysis of the A-esterase and B-esterase using polyacrylamide gel electrophoresis (PAGE) have shown differential profiles.
Ganesh
K N
KN
Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore 570 006, India.
Vijayan
V A
VA
Urmila
J
J
Gopalan
N
N
Prakash
Shri
S
D000818:Animals; D000852:Anopheles; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000378:metabolism*; D007305:Insecticide Resistance; D007306:Insecticides; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D009570:Nitriles; D011722:Pyrethrins
D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D006899:Mixed Function Oxygenases; D004950:Esterases