Differential functional properties of calmodulin-dependent protein kinase IIgamma variants isolated from smooth muscle.
Six variants of calmodulin-dependent protein kinase IIgamma were isolated from a ferret-aorta smooth-muscle cDNA library. Variant G-2 is generated by a novel alternative polyadenylation, utilizing a site contained in an intron. The last 77 residues of the association domain are replaced with 99 residues of a unique sequence containing Src homology 3-domain-binding motifs, which alter catalytic activity. Variant C-2 has an eight-residue deletion in an ATP-binding motif and does not autophosphorylate Thr(286), but does phosphorylate exogenous substrate. Two variants, B and J, autodephosphorylate. Four variants differing only in the variable domain have differing catalytic activities, despite identical sequences in the catalytic domains. Thus structural features determined by variable and association domains are important for the catalytic activity of calmodulin-dependent protein kinase II.
Gangopadhyay
Samudra S
SS
Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA.
Barber
Amy L
AL
Gallant
Cynthia
C
Grabarek
Zenon
Z
Smith
Janet L
JL
Morgan
Kathleen G
KG
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000201:enzymology; D001665:Binding Sites; D015152:Blotting, Northern; D019556:COS Cells; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000302:isolation & purification* / Q000502:physiology*; D002147:Calmodulin / Q000378:metabolism; D002384:Catalysis; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D005289:Ferrets; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D010957:Plasmids; D026723:Polyadenylation; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D011817:Rabbits; D011994:Recombinant Proteins / Q000378:metabolism; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013912:Threonine / Q000737:chemistry
D002147:Calmodulin; D017931:DNA Primers; D020033:Protein Isoforms; D011994:Recombinant Proteins; D013912:Threonine; C517662:CAMK2G protein, human; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases