Angiotensin I-converting enzyme inhibitory peptides in a hydrolyzed chicken breast muscle extract.
The blood pressure of spontaneously hypertensive rats (SHRs) decreased after oral administration of an extract prepared from chicken breast muscle, falling maximally to 50 mmHg lower than before. This effect continued for at least 4 h after administration. The peptides possessing hypotensive activity in the chicken extract were examined by measuring the inhibitory activity (IC(50)) against angiotensin I-converting enzyme (ACE). The inhibitory activity of the chicken extract was 1060 mg%, whereas the activity of the extract treated with an Aspergillus protease and gastric proteases (trypsin, chymotrypsin, and intestinal juice) became stronger, reaching 1.1 mg%. Peptides in this hydrolysate of the extract were isolated by HPLC on a reversed-phase column, and their N-terminal sequences were analyzed. Three peptides possessed a common sequence, Gly-X-X-Gly-X-X-Gly-X-X, which was homologous with that of collagen. The peptide Gly-Phe-Hyp-Gly-Thr-Hyp-Gly-Leu-Hyp-Gly-Phe showed the strongest inhibitory activity (IC(50) = 42 microM).
Saiga
Ai
A
Graduate School of Biosphere, Hiroshima University, Higashi-hiroshima 739-8528, Japan.
Okumura
Tomoyuki
T
Makihara
Toshikazu
T
Katsuta
Shinichiro
S
Shimizu
Tsuyoshi
T
Yamada
Ryoji
R
Nishimura
Toshihide
T
D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis* / Q000494:pharmacology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D010450:Endopeptidases / Q000378:metabolism; D006868:Hydrolysis; D007421:Intestine, Small / Q000737:chemistry; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry*; D009842:Oligopeptides / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D010455:Peptides / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D011918:Rats, Inbred SHR; D013552:Swine; D014020:Tissue Extracts / Q000737:chemistry* / Q000494:pharmacology
D000806:Angiotensin-Converting Enzyme Inhibitors; D009842:Oligopeptides; D010455:Peptides; D014020:Tissue Extracts; D010450:Endopeptidases