Immunocytolocalization of tryptophan decarboxylase in Catharanthus roseus hairy roots.
We investigated the intracellular distribution of tryptophan decarboxylase (TDC) (EC 4.1.1.28) in Catharanthus roseus hairy roots using immunofluorescence and immunogold techniques. TDC was detected by immunofluorescence localization in the cytosol and in the apoplastic region of the meristematic cells of the roots, with a slight enrichment in the epidermal cells of the root cap and in the meristematic region. In the enlargement zone, TDC was localized only in the first three layers of the cortex. In the maturation zone, the enzyme was not present. Immunogold studies confirmed that the enzyme was localized in the cytosol of the meristematic region, and intense gold labeling was found in the apoplastic zone. A protein fraction isolated from the apoplastic zone and assayed for TDC activity showed high activity.
Moreno-Valenzuela
Oscar A
OA
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de investigacíon Científica de Yucatán, Calle 43, número 130, CP 97200, Mérida, Yucatán, México.
Minero-García
Yereni
Y
Brito-Argáez
Ligia
L
Carbajal-Mora
Eloísa
E
Echeverría
Olga
O
Vázquez-Nin
Gerardo
G
Loyola-Vargas
Víctor M
VM
D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000737:chemistry* / Q000378:metabolism*; D030008:Catharanthus / Q000737:chemistry / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D046508:Culture Techniques / Q000379:methods; D004789:Enzyme Activation; D005455:Fluorescent Antibody Technique; D018517:Plant Roots / Q000737:chemistry / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure
D001142:Aromatic-L-Amino-Acid Decarboxylases