Ascorbic acid potentiates mitomycin C-induced micronuclei and sister chromatid exchanges in human peripheral blood lymphocytes in vitro.
Vitamin C (l-ascorbic acid), an effective free radical scavenger present as ascorbate in most biological systems, is one of the most extensively studied antioxidant vitamins. Vitamin C acts as either a free radical scavenger or a pro-oxidant producing hydrogen peroxide and free radicals. The modulatory effect of L-ascorbic acid (AA) on Mitomycin C (MMC) induced chromosome damage has been evaluated in human peripheral blood lymphocytes in vitro. The effect of L-ascorbic acid, 200 microg/ml as 1- and 2-h pretreatment on the frequencies of the biomarkers micronuclei (MN), sister chromatid exchanges (SCEs), and chromosome aberrations (CA) induced by mitomycin C 0.1 and 0.2 microg/ml has been studied. AA pretreatment caused a statistically significant increase in MMC-induced MN and SCE frequencies for all treatment groups, but did not show an increase in induced chromosome aberrations compared to MMC treatment alone. Cell division delays caused by MMC was reversed in the presence of AA. Interindividual variability in MMC as well as AA plus MMC-induced MN, SCE, and CA frequencies were evident. Ascorbic acid potentiated MMC-induced chromosome damage in human lymphocytes in vitro. The potentiation observed has to be viewed in the light of metal ion catalysed autooxidation of AA in oxygenated media and the existence of an antioxidant system in vivo that inactivates oxyradicals before their interaction with DNA.
Krishnaja
A P
AP
Genetic Toxicology and Chromosome Studies Section, Cell Biology Division, Bhabha Atomic Research Centre, Mumbai, India. krishnja@apsara.barc.ernet.in
Sharma
N K
NK
D000328:Adult; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology*; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000139:chemically induced*; D004307:Dose-Response Relationship, Radiation; D004357:Drug Synergism; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests / Q000379:methods; D016685:Mitomycin / Q000494:pharmacology*; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D000903:Antibiotics, Antineoplastic; D016166:Free Radical Scavengers; D016685:Mitomycin; D001205:Ascorbic Acid