Application of attenuated total reflectance Fourier transform infrared spectrometry to the determination of cephalosporin C in complex fermentation broths.
An analytical procedure has been developed for quantitative determination of cephalosporin C in complex fermentation broths. The method is based on the partial least-square treatment of data obtained by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectrometric method in the wavenumber range of 1227-1257 cm(-1). Absorbance spectra were employed for measurement using a set of eleven binary aqueous standard solutions of cep halosporin C and deacetoxycephalosporin C. The method is simple, rapid and accurate (to within +/-1.49%). The developed method has been used to measure cephalosporin C in aqueous solution of biosynthetic samples only after freeze drying the sample at -30 degrees C and dissolving it in distilled water.
Kargosha
K
K
Chemistry and Chemical Engineering Research Center of Iran, PO Box 14335-186, Tehran, Iran. k_kargosha@yahoo.com
Khanmohammadi
M
M
Sarokhani
M
M
Ansari
F
F
Ghadiri
M
M
D002138:Calibration; D002511:Cephalosporins / Q000032:analysis* / Q000096:biosynthesis; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D005285:Fermentation; D007202:Indicators and Reagents; D012015:Reference Standards; D012984:Software; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
D002511:Cephalosporins; D003470:Culture Media; D007202:Indicators and Reagents; D012996:Solutions; C025163:cephalosporin C