Yeast inositol mono- and trisphosphate levels are modulated by inositol monophosphatase activity and nutrients.
Yeast lithium-sensitive inositol monophosphatase (IMPase) is encoded by a non-essential gene pair (IMP1 and IMP2). Inhibition of IMPase with either Li(+) or Na(+) or a double null mutation imp1 imp2 causes increased levels of inositol monophosphates and reduced level of inositol 1,4,5-trisphosphate. Overexpression of the IMP2 gene has the opposite effects and these results suggest that IMPase activity is limiting for the inositol cycle. Addition of ammonium to cells starved for this nutrient results in a decrease of inositol monophosphates and an increase of inositol 1,4,5-triphosphate, pointing to simultaneous regulation of both inositol 1,4,5-triphosphate production and IMPase activity.
Navarro-Aviñó
Juan P
JP
Instituto de Biologia Molecular y Celular de Plantas, Universidad Politécnica de Valencia-C.S.I.C., Camino de Vera s/n, Valencia 46022, Spain.
Bellés
José M
JM
Serrano
Ramón
R
D003470:Culture Media; D007295:Inositol Phosphates / Q000378:metabolism*; D009154:Mutation; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
D003470:Culture Media; D007295:Inositol Phosphates; D010744:Phosphoric Monoester Hydrolases; C021822:myo-inositol-1 (or 4)-monophosphatase