title: Effects of oils and pharmaceutical excipients on the bioavailability of ampicillin orally administered, different oily and aqueous suspensions in rabbit.
abstract: The in vivo bioavailability and in vitro drug-release studies of ampicillin trihydrate in different oily and aqueous suspensions have been investigated. In addition, partition, solubility, and rheological measurements have also been carried out. The in vivo experimental design was based on a 6 x 6 latin square using the rabbit as the test animal. The bioavailability of ampicillin was determined using the plasma levels, which were measured microbiologically. Results of the study showed that oily and sucrose-containing aqueous formulations enhanced the extent of ampicillin absorption, although not statistically significantly, but was close to the borderline of significance. Ampicillin appears to be absorbed at essentially the same rate from both aqueous and oily formulations. The latter showed plasma-level time curves with biphasic absorption and are likely to produce prolonged plasma concentrations of ampicillin because of the effects of enterohepatic recycling. Viscosity appears to play an insignificant role in the results obtained since the bioavailability parameters correlate poorly with the viscosity except Cmax. It is suggested that enhancement in the bioavailability of ampicillin is due to the decrease in the gut transit rate brought about by the oil which predominates and masks the other effects of viscosity and osmotic effects of sucrose. The existence of a correlation between the in vitro drug-release rate (t50%) and viscosity and the lack of a correlation between in vivo and in vitro parameters support the above suggestion and indicate that traditional dissolution rate tests, such as flask-stirrer method, are unsatisfactory as bioavailability indicators when applied to dosage forms that caused marked changes in physiological factors like GER and biliary excretion.
authors.affiliation: De Montfort University of Leicester, UK. oalhamami@hotmail.com
authors.forename: Omran M O
authors.initials: OM
authors.lastname: Alhamami
mesh_terms: D000284:Administration, Oral; D000667:Ampicillin / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D000818:Animals; D019540:Area Under Curve; D001682:Biological Availability; D000074263:Coconut Oil; D005079:Excipients; D005772:Gastrointestinal Transit / Q000187:drug effects; D006207:Half-Life; D019999:Pharmaceutical Solutions; D014677:Pharmaceutical Vehicles; D010938:Plant Oils; D011817:Rabbits; D012212:Rheology; D013535:Suspensions; D014783:Viscosity
chemical_list: D005079:Excipients; D019999:Pharmaceutical Solutions; D014677:Pharmaceutical Vehicles; D010938:Plant Oils; D013535:Suspensions; D000667:Ampicillin; D000074263:Coconut Oil