Turnip crinkle virus coat protein mediates suppression of RNA silencing in Nicotiana benthamiana.
All of the protein products of Turnip crinkle virus (TCV; Tombusviridae, Carmovirus) were tested for their ability to suppress RNA silencing of a reporter gene after transient expression in Agrobacterium-infiltrated Nicotiana benthamiana leaves. Only the capsid protein, P38, showed suppression activity, although this was not obvious when P38 was expressed as part of a TCV infection of the same tissues. When P38 was expressed from a PVX vector, symptoms with enhanced severity that correlated with increased PVX RNA accumulation were observed. This contradiction between ectopic expression of P38 and TCV infection could be accounted for if the active determinant of suppressor activity within P38 was sequestered within the capsid protein structure. The N-terminal 25 amino acids were shown to be important for this activity. This region forms part of the unexposed R-domain that interacts with the RNA within the virus particle. This observation throws light on some of the complex biology exhibited by TCV.
Thomas
Carole L
CL
John Innes Centre, Norwich Research Park, NR4 7UH, Colney, Norwich, UK.
Leh
V
V
Lederer
Carsten
C
Maule
Andrew J
AJ
D029688:Brassica napus / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D019184:Carmovirus / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D015967:Gene Expression Regulation, Viral; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000821:virology*
D036022:Capsid Proteins; D008164:Luminescent Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins