title: Unilamellar liposomes covalently coupled on silica gel for liquid chromatography.
abstract: Silica gel was used as a support for the covalent coupling of liposomes, which could overcome drawbacks of soft gel beads in column efficiency and separation speed. The influences of the concentration of added dimethylaminopyridine and reaction time on the chloroformate activation reaction of silica gel were investigated. Temperature and pH for covalent coupling of liposomes on the activated silica gel were also optimized. Experimental results indicated that the stability of the covalently coupled liposome columns was obviously superior to that of the noncovalently coated liposome columns but the selectivity of both columns was basically identical. Separation and analysis of a crude extract of a traditional Chinese medicine Ligusticum Wallichii and a mixture of small peptides on both columns further support this conclusion.
authors.affiliation: National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116011, Dalian, China.
authors.forename: Xiqin
authors.initials: X
authors.lastname: Mao
authors.forename: Liang
authors.initials: L
authors.lastname: Kong
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Baochuan
authors.initials: B
authors.lastname: Guo
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification; D030004:Ligusticum / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D008081:Liposomes; D010455:Peptides; D010936:Plant Extracts; D058428:Silica Gel; D012822:Silicon Dioxide