title: Dietary lipids alter the effect of steroids on the transport of glucose after intestinal resection: Part I. Phenotypic changes and expression of transporters.
abstract: OBJECTIVE
  Glucocorticosteroids alter the function of the intestine. This study was undertaken to assess the effect on D-glucose uptake of budesonide (Bud), prednisone (Pred), or dexamethasone (Dex) in animals with a 50% intestinal resection and fed chow or a diet enriched with saturated (SFA) or polyunsaturated fatty acids (PUFA).
  
  
  METHODS
  In vitro ring uptake technique, Western blots, and Northern blots were performed.
  
  
  RESULTS
  Bud increased the jejunal D-glucose uptake, and this effect was prevented by feeding PUFA. SGLT1 and Na+/K+ ATPase protein and mRNA abundance did not correlate with the change in the rate of uptake of glucose.
  
  
  CONCLUSIONS
  (1) Bud increased the jejunal glucose uptake, (2) the activity of the sugar transporter does not correlate with the abundance of protein or their respective mRNAs, (3) th Bud effect on glucose uptake is prevented by feeding PUFA. Thus, the desired intestinal adaptive response after intestinal resection may be enhanced further by the administration of the locally acting steroid budesonide and by feeding a saturated compared with a polyunsaturated fatty acid diet.
authors.affiliation: Nutrition and Metabolism Research Group, Division of Gastroenterology, Department of Medicine, University of Alberta, Edmonton, Canada.
authors.forename: Aducio L
authors.initials: AL
authors.lastname: Thiesen
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Tappenden
authors.forename: Mike I
authors.initials: MI
authors.lastname: McBurney
authors.forename: M Thomas
authors.initials: MT
authors.lastname: Clandinin
authors.forename: Monika
authors.initials: M
authors.lastname: Keelan
authors.forename: Ben K
authors.initials: BK
authors.lastname: Thomson
authors.forename: Gary E
authors.initials: GE
authors.lastname: Wild
authors.forename: Alan B
authors.initials: AB
authors.lastname: Thomson
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D019819:Budesonide / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004041:Dietary Fats / Q000008:administration & dosage*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage; D005786:Gene Expression Regulation / Q000187:drug effects; D005938:Glucocorticoids / Q000494:pharmacology*; D005947:Glucose / Q000493:pharmacokinetics*; D007082:Ileum / Q000187:drug effects / Q000502:physiology / Q000601:surgery*; D007408:Intestinal Absorption / Q000187:drug effects; D007583:Jejunum / Q000187:drug effects / Q000502:physiology / Q000601:surgery*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D011241:Prednisone / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D051269:Sodium-Glucose Transporter 1; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects
chemical_list: D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated; D005231:Fatty Acids, Unsaturated; D005938:Glucocorticoids; D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; C495676:Slc5a1 protein, rat; D051269:Sodium-Glucose Transporter 1; D019819:Budesonide; D003907:Dexamethasone; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose; D011241:Prednisone