[Encoding genes and genotypes of gamma-lactamases produced by a multiple resistant Klebsiella pneumoniae]
OBJECTIVE: To determine the sequence of gene for encoding beta-lactamase produced by Klebsiella pneumoniae E3 isolated from Jiaxing Area in Zhejiang Province. METHODS The Klebsiella pneumoniae strain E3 was identified as an ESBLs-producing bacterium by inhibitor-potentiated broth dilution test. The gene encoding gamma-lactamase of the strain was amplified by PCR. The purified PCR product was cloned and sequenced by Sanger's dideoxy chain termination composition method. RESULTS The Klebsiella pneumoniae strain E3 produced both TEM and SHV gamma lactamases. The SHV encoding gene had 812 nucleotide residues responsible for encoding SHV-11 gamma-lactamase and the TEM encoding gene had 973 nucleotide residues responsible for encoding TEM-1 gamma-lactamase. CONCLUSION The Klebsiella pneumoniae strain E3 isolated from a patient in Jiaxing Area in Zhejiang Province is able to produce both TEM-1 and SHV-11 gamma-lactamases.
Yu
Yun-Song
YS
The First Affiliated Hospital, College of Medical Science, Zhejiang University, Hangzhou 310003, China.
Lu
Fang-Fang
FF
Song
Xiu-Lan
XL
Chen
Ya-Gang
YG
Zhou
Wei-Lin
WL
Ma
Yi-Lin
YL