A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis.
The bHLH transcription factor, Hand1 has been implicated in cardiac looping in the mouse, however its function in the developing heart remains unknown. To investigate the mechanism(s) through which Hand1 might function, we screened for potential downstream target genes using representational difference analysis. Thymosin beta4 was found to be down-regulated whereas cystatin C and alphaCA were up-regulated in Hand1-null embryoid bodies. Whole-mount in situ hybridisation on wild type embryos (E8.0-E10.5) and Hand1 homozygous-mutant embryos (E8.0) confirmed co-expression of the putative targets with Hand1 in the heart and their aberrant expression in a Hand1-null background.
Smart
Nicola
N
Molecular Medicine Unit, Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK.
Hill
Alison A
AA
Cross
James C
JC
Riley
Paul R
PR
D051792:Basic Helix-Loop-Helix Proteins; D055316:Cystatin C; D015891:Cystatins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006321:Heart / Q000196:embryology*; D017403:In Situ Hybridization; D009206:Myocardium / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
D051792:Basic Helix-Loop-Helix Proteins; D055316:Cystatin C; D015891:Cystatins; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C525031:Cst3 protein, mouse; C097341:helix-loop-helix protein, eHAND