Complement component anaphylatoxins upregulate chemokine expression by human astrocytes.
The complement (C) system, a major component of the innate immune system, has been described as a factor implicated in some brain disorders. C activation leads to the release of anaphylatoxins, two proinflammatory polypeptides acting through specific receptors that have been detected on brain cells. Here, we examined the effect of anaphylatoxins on chemokine expression by human astrocytes. We showed that anaphylatoxins significantly increase chemokine mRNA expression. However, anaphylatoxin-induced chemokine secretion (interleukin-8) was observed only in the presence of interleukin-1beta. Thus, anaphylatoxins could initiate a chemokine cascade and, at least in part, be involved in pathogenesis of the brain.
Jauneau
Anne-Christine
AC
Institut Fédératif de Recherche Multidisciplinaire sur les Peptides No. 23, INSERM U519, Faculté Mixte de Médecine et de Pharmacie, 22 Boulevard Gambetta, 76183 Rouen Cedex, France. acjauneau@hotmail.com
Ischenko
Alexander
A
Chan
Philippe
P
Fontaine
Marc
M
D000706:Anaphylatoxins / Q000494:pharmacology*; D001253:Astrocytes / Q000276:immunology*; D018925:Chemokines / Q000235:genetics*; D015926:Complement C3a / Q000494:pharmacology; D015936:Complement C5a / Q000494:pharmacology; D003165:Complement System Proteins / Q000494:pharmacology*; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics; D016133:Polymerase Chain Reaction; D012260:Ribonucleases
D000706:Anaphylatoxins; D018925:Chemokines; D017931:DNA Primers; D016209:Interleukin-8; D015926:Complement C3a; D015936:Complement C5a; D003165:Complement System Proteins; D012260:Ribonucleases