Plasmid DNA electrotransfer: a new non viral method for gene therapy in oncology.
With some defined conditions, electric pulses delivery to tissue in vivo can greatly enhance DNA transfection. We here describe the application in oncology of intratumoral or intramuscular DNA electrotransfer by using muscle as a secretory tissue of transgenic proteins displaying anticancer properties.
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UMR 7001 CNRS-ENSCP / Aventis, CRVA 13 quai Jules Guesde 94403, Vitry sur Seine, France.
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D000230:Adenocarcinoma / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000628:therapy; D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000628:therapy; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000628:therapy; D003110:Colonic Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000628:therapy; D004273:DNA, Neoplasm / Q000008:administration & dosage*; D004560:Electricity; D005260:Female; D005354:Fibrosarcoma / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000628:therapy; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D018374:LLC-PK1 Cells; D008156:Luciferases / Q000235:genetics; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000628:therapy; D008546:Melanoma, Experimental / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000628:therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D009368:Neoplasm Transplantation / Q000379:methods; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D008156:Luciferases