title: Pre- and postsynaptic inhibition mediated by GABA(B) receptors in rat ventrolateral periaqueductal gray neurons.
abstract: The present study examined the actions of a GABA(B)-receptor agonist, baclofen, on synaptic transmission in rat ventrolateral periaqueductal gray (PAG) neurons of brainstem slices by using whole-cell voltage-clamp recordings. Baclofen (10 microM) induced a slow outward current (peak amplitude: 30.1+/-3.1pA, n=13) at -70mV, which persisted in the presence of tetrodotoxin (0.5 microM) and was diminished in the presence of postsynaptic intracellular K(+)-channel blockers (Cs(+) and TEA) and GDP-beta-S, indicating a direct postsynaptic depression mediated by K(+) channels and G proteins. Baclofen (10 microM) also decreased the frequency of both glutamatergic spontaneous EPSC (by 36+/-7%, n=11) and GABAergic spontaneous IPSC (by 37+/-12%, n=6) without changes in their amplitudes, indicating its presynaptic inhibitions. Taken together, the activation of postsynaptic GABA(B) receptors inhibits ventrolateral PAG neurons directly. At the same time, activating presynaptic GABA(B) receptors on glutamatergic and GABAergic nerve terminals inhibits glutamate and GABA release, respectively. The overall effects might influence an output of ventrolateral PAG neurons that build up the descending pain control system to the spinal dorsal horn.
authors.affiliation: Department of Integrative Physiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. yankun@post.saga-med.ac.jp
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Furue
authors.forename: Eiichi
authors.initials: E
authors.lastname: Kumamoto
authors.forename: Yuan-Xiang
authors.initials: YX
authors.lastname: Dong
authors.forename: Megumu
authors.initials: M
authors.lastname: Yoshimura
mesh_terms: D000818:Animals; D008297:Male; D009474:Neurons / Q000502:physiology*; D010487:Periaqueductal Gray / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018080:Receptors, GABA-B / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D018080:Receptors, GABA-B