title: A 2-oxoglutarate-dependent dioxygenase is integrated in DIMBOA-biosynthesis.
abstract: Benzoxazinoids are secondary metabolites of grasses that function as natural pesticides. While many steps of DIMBOA biosynthesis have been elucidated, the mechanism of the introduction of OCH(3)-group at the C-7 position was unknown. Inhibitor experiments in Triticum aestivum and Zea mays suggest that a 2-oxoglutarate-dependent dioxygenase catalyses the hydroxylation reaction at C-7. Cloning and reverse genetics analysis have identified the Bx6 gene that encodes this enzyme. Bx6 is located in the Bx-gene cluster of maize.
authors.affiliation: Lehrstuhl für Genetik, Technische Universität München, 85350 Freising, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Frey
authors.forename: Karin
authors.initials: K
authors.lastname: Huber
authors.forename: Woong June
authors.initials: WJ
authors.lastname: Park
authors.forename: Dieter
authors.initials: D
authors.lastname: Sicker
authors.forename: Pia
authors.initials: P
authors.lastname: Lindberg
authors.forename: Robert B
authors.initials: RB
authors.lastname: Meeley
authors.forename: Carl R
authors.initials: CR
authors.lastname: Simmons
authors.forename: Nasser
authors.initials: N
authors.lastname: Yalpani
authors.forename: Alfons
authors.initials: A
authors.lastname: Gierl
mesh_terms: D000595:Amino Acid Sequence; D048588:Benzoxazines; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019143:Evolution, Molecular; D006900:Hydroxylation; D007656:Ketoglutaric Acids / Q000378:metabolism*; D008745:Methylation; D008969:Molecular Sequence Data; D009154:Mutation; D010078:Oxazines / Q000378:metabolism*; D010105:Oxygenases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D014908:Triticum / Q000201:enzymology; D003313:Zea mays / Q000201:enzymology / Q000235:genetics
chemical_list: D048588:Benzoxazines; D004251:DNA Transposable Elements; D004791:Enzyme Inhibitors; D007656:Ketoglutaric Acids; D010078:Oxazines; D010105:Oxygenases; C050733:2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one