title: Evaluation of the effect of mitomycin-C on the bioavailability of technetium-99m-labelled sodium pyrophosphate in mice.
abstract: We have reported that drugs alter the biodistribution of radiopharmaceuticals used in diagnostic imaging in nuclear medicine. Knowledge of such altered biodistribution is important in making diagnostic from scintigraphy. Mitomycin-C is used as component of many chemotherapeutic regimens to treat different tumors. The biological activities of mitomycin-C can be explained by its ability to inhibit deoxyribonucleic acid synthesis. Since patients on chemotherapeutic treatment can be submitted to nuclear medicine procedures, we studied the mitomycin-C effect on the bioavailability of the technetium-99m-labelled sodium pyrophosphate (9mTc-PYP) using an animal model. Mitomycin (0.45 mg) was administered by ocular plexus way Balb/c mice. One hour after the last dose, 99mTc-PYP (7.4 MBq) was administered and after 0.5 hr the animals (n = 15) were rapidly sacrificed. The organs were isolated, the radioactivity counted in a well counter and the percentage of radioactivity (%ATI) calculated. The results have shown that in the treated animals the %ATI has been decreased in spleen, thymus, heart and brain and increased in lung, liver and bone. The effect of this chemotherapeutic drug on the 99mTc-PYP biodistribution was statistically significant (Wilcoxon test, p < 0.05) and it could be explained by the metabolization or therapeutic action of mitomycin-C.
authors.affiliation: Universidade do Estado do Rio de Janeiro, Instituto de Biologia, Departamento de Biofisica e Biometria, Av. 28 de Setembro, 87, Rio de Janeiro, RJ, Brazil.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Gomes
authors.forename: Deise Mara M
authors.initials: DM
authors.lastname: de Mattos
authors.forename: Rosimeire S
authors.initials: RS
authors.lastname: Freitas
authors.forename: Glaucio F
authors.initials: GF
authors.lastname: Diré
authors.forename: Elaine A C
authors.initials: EA
authors.lastname: Lima
authors.forename: Sonia Maria S
authors.initials: SM
authors.lastname: Souza
authors.forename: Mario
authors.initials: M
authors.lastname: Bernardo-Filho
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology / Q000633:toxicity; D001682:Biological Availability; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D016685:Mitomycin / Q000494:pharmacology* / Q000633:toxicity; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D016698:Technetium Tc 99m Pyrophosphate / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D000903:Antibiotics, Antineoplastic; D019275:Radiopharmaceuticals; D016685:Mitomycin; D016698:Technetium Tc 99m Pyrophosphate