[Effects of beech dust extract on lipid peroxidation and expression of ref-1 gene of mouse lung tissue].
In order to explore the effects of beech dust on lipid peroxidation and expression of redox-related gene ref-1, the 2-stage model of mouse lung tumor short-term induction test is used. The mice exposed to the extract beech dust are divided into 3 groups(group of normal lung tissue, group of papillary adenoma and group of lung adenoma) with the mice treated with solvents as control. The extent of homogenate lipid peroxides is determined by measuring the formation of TBARS, and expression of ref-1 measured by immunohistochemistry. The results show that 1. The formation of TBARS of all groups treated with the beech extracts of beech dust is increased compared with the control, and the differences between the groups with adenoma and the control are statistically significant (P < 0.01), while the P value of that of the group of normal lung tissue is less than 0.05. 2. Both the control and the group of normal lung tissue treated with the extracts are Ref-1-negative, strong nuclear Ref-1 immunostaining is found in the group of papillary adenoma, and the cytoplasmic/nuclear expression of Ref-1 is significantly enhanced in the group of lung adenoma and group of mixed adenoma. It is suggested that the extracts of beech dust toxicity is probably related to its altering redox regulation of ref-1 and subsequent disturbance of redox status.
He
Peng
P
Institute of Occupational Medicine, Chinese Academy of Preventive Medicine, Beijing 100050, China.
Lü
Xiaohua
X
Wu
Desheng
D
Dong
Qinan
Q
D000236:Adenoma / Q000139:chemically induced / Q000378:metabolism; D000818:Animals; D019757:Carbon-Oxygen Lyases / Q000096:biosynthesis* / Q000235:genetics; D004249:DNA Damage; D004260:DNA Repair; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D004391:Dust; D029964:Fagus; D005260:Female; D015227:Lipid Peroxidation; D008168:Lung / Q000378:metabolism*; D008175:Lung Neoplasms / Q000139:chemically induced / Q000378:metabolism; D008297:Male; D051379:Mice; D010936:Plant Extracts
D004391:Dust; D010936:Plant Extracts; D019757:Carbon-Oxygen Lyases; C486216:Apex1 protein, mouse; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase