Babesia gibsoni: molecular cloning and characterization of Rab6 and Rab11 homologues.
Members of the Rab subfamily of GTPases have been implicated as important components in vesicle trafficking in the eukaryotes, individual Rab proteins have a remarkable degree of specific subcellular localization. As a first step towards developing a set of compartment specific probes for studying protein trafficking in Babesia-infected erythrocyte, here we describe the cloning and characterization of Rab6 and Rab11 gene homologues in Babesia gibsoni (BgRab6 and BgRab11). The deduced amino acid sequence of both BgRab6 and BgRab11 contained the highly conserved GTP-binding consensus sequence and C-terminal cysteines. Northern blotting analysis of total RNA hybridized a 1.3 kb band on both BgRab6 and BgRab11 probed blots consistent with the expected size. Using a GTP-binding assay we demonstrated that Escherichia coli expressed recombinant BgRab6 and BgRab11 were able to bind GTP. BgRab6 and BgRab11 represent the first two molecular markers of B. gibsoni.
Zhou
Jinlin
J
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Mulenga
Albert
A
Yamasaki
Masahiro
M
Ohashi
Kazuhiko
K
Maede
Yoshimitsu
Y
Onuma
Misao
M
D000595:Amino Acid Sequence; D000818:Animals; D001403:Babesia / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D015152:Blotting, Northern; D003001:Cloning, Molecular; D016384:Consensus Sequence; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry; D004285:Dogs; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D021381:Protein Transport / Q000235:genetics; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016053:RNA, Protozoan / Q000032:analysis / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D020691:rab GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D000099193:rab11 GTP-Binding Proteins
D001798:Blood Proteins; D018076:DNA, Complementary; D016054:DNA, Protozoan; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C094135:Rab6 protein