Radioiodination of proteins using prosthetic group: a convenient way to produce labelled proteins with in vivo stability.
Radiolabelled peptides can provide new approaches for radiopharmaceutical development. Several prosthetic groups have been developed for radioiodination of proteins in order to minimize in vivo dehalogenation. In this work, the prosthetic group N-succinimidyl 4-[131I]iodobenzoate ([131I]SIB) was obtained by an alternative procedure that employs Cu(I) assisted radioiododebromination to produce p-[131I]iodobenzoic acid with a radiochemical yield of 92.73 +/- 1.51% (N = 6), followed by the reaction with TSTU (O-(N-succinimidyl)-N,N,N'N'-tetramethyluronium) in alkaline medium. The HPLC profile of the final product, revealed that [131I]SIB was obtained with a radiochemical purity of 98.19 +/- 1.14% (N = 6 Swiss mices (normal group) and animals with inflammation focus developed on the right thigh by tupertine injection) were injected with human immunoglobulin (IgG) radioiodinated with [131I]SIB and by direct method (Iodogen). The comparison of results showed a fast blood clearance, better target organ/background relation and low uptake in thyroid and stomach (p < 0.01) for the protein labelled with [131I]SIB, what suggests a greater in vivo stability.
Santos
J S
JS
Instituto de Pesquisas Energéticas e Nucleares (IPEN)-CNEN/SP, São Paulo, Brazil.
Muramoto
E
E
Colturato
M T
MT
Siva
C P G
CP
Araújo
E B
EB
D000818:Animals; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000302:isolation & purification; D007249:Inflammation / Q000000981:diagnostic imaging; D007457:Iodine Radioisotopes / Q000737:chemistry* / Q000302:isolation & purification; D007463:Iodobenzoates; D051379:Mice; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000737:chemistry* / Q000302:isolation & purification; D014018:Tissue Distribution
D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D007463:Iodobenzoates; D011506:Proteins; D019275:Radiopharmaceuticals; C062338:N-succinimidyl 4-iodobenzoate