Ovarian modulators of 11beta-hydroxysteroid dehydrogenase (11beta HSD) activity in follicular fluid from bovine and porcine large antral follicles and spontaneous ovarian cysts.
In the ovary, cortisol is oxidized to cortisone by 11beta-hydroxysteroid dehydrogenase (11betaHSD). The present study investigated whether follicular fluid (FF) from large antral follicles and spontaneous ovarian cysts, isolated from bovine and porcine ovaries, contained modulators of 11betaHSD activity. Whereas FF from antral follicles had no significant effect over 1 h on NADP+-dependent 11betaHSD activity in rat kidney homogenates, enzyme activity was inhibited by FF from bovine and porcine ovarian cysts (80.5% +/- 2.3% and 72.8% +/- 3.4% of control, respectively). Following C18 reverse-phase chromatography, the hydrophilic fractions of FF from bovine and porcine antral follicles stimulated NADP+-dependent 11betaHSD activities (111.5% +/- 21.6% and 55.2% +/- 5.7% respectively). Hydrophobic compounds inhibited NADP+-dependent cortisol oxidation by 58.2% +/- 5.1% (bovine) and 45.7% +/- 2.0% (porcine). In both species, FF from ovarian cysts appeared to contain less of the hydrophilic stimuli to 11betaHSD activity and more of the hydrophobic inhibitors. The FF from antral follicles and ovarian cysts, and the C18 fractions thereof, had no significant effect on NAD+-dependent cortisol oxidation. The ovarian modulators of NADP+-dependent 11betaHSD activities did not coelute with cortisol, cortisone, estradiol, testosterone, progesterone, pregnenolone, and cholesterol. However, the 11betaHSD stimuli in porcine FF from both antral follicles and cysts coeluted with prostaglandin (PG) E2 and PGF2alpha. We conclude that large antral follicles and spontaneous ovarian cysts, in both the cow and the pig, contain ovarian modulators of the NADP+-dependent 11betaHSD activity. Moreover, FF from spontaneous ovarian cysts, because of decreased content of the 11betaHSD stimulus accompanied by increased content of the 11betaHSD inhibitors, exerts a net inhibitory effect on 11betaHSD activity.
Thurston
Lisa M
LM
Department of Veterinary Basic Sciences, Royal Veterinary College, Camden Town, London NW1 0TU, United Kingdom. lthurston@rvc.ac.uk
Jonas
Kim C
KC
Abayasekara
D Robert E
DR
Michael
Anthony E
AE
D042842:11-beta-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D005260:Female; D015571:Follicular Fluid / Q000201:enzymology*; D012739:Gonadal Steroid Hormones / Q000502:physiology; D006854:Hydrocortisone / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D009243:NAD / Q000502:physiology; D009249:NADP / Q000502:physiology; D010048:Ovarian Cysts / Q000201:enzymology*; D006080:Ovarian Follicle / Q000201:enzymology*; D010053:Ovary / Q000502:physiology*; D010084:Oxidation-Reduction; D013552:Swine
D012739:Gonadal Steroid Hormones; D009243:NAD; D009249:NADP; D042842:11-beta-Hydroxysteroid Dehydrogenases; D006854:Hydrocortisone