Improvements in lipase production and recovery from Acinetobacter radioresistens in presence of polypropylene powders filled with carbon sources.
Polypropylene powders as the adsorbent for organic solution containing n-hexadecane and olive oil were employed as the carbon source for producing an alkaline lipase from Acinetobacter radioresistens. The best volumetric ratio of n-hexadecane to olive oil around 5 for lipase production was determined from shake-flask and fermentation cultivations. The existence of a maximum time course lipase activity of the aqueous phase was attributed to the compensation effects of olive oil on cell growth and lipase production, repression of lipase synthesis by oleic acid, and lipase adsorption on the supports. A linear relationship between the average cell growth rate in the exponential phase and the ratio of surface areas of the supports was found. The benefits of using the present fermentation process include less foaming and emulsion of the broth, less organic phase used, higher lipase production, and easy recovery of the lipase in the centrifugation step.
Liu
In-Liang
IL
Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan 70101, Republic of China.
Tsai
Shau-Wei
SW
D000150:Acinetobacter / Q000145:classification / Q000201:enzymology* / Q000254:growth & development*; D000473:Alkanes / Q000378:metabolism; D002244:Carbon; D002478:Cells, Cultured; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000096:biosynthesis / Q000302:isolation & purification; D008049:Lipase / Q000096:biosynthesis* / Q000302:isolation & purification*; D008567:Membranes, Artificial; D000069463:Olive Oil; D010938:Plant Oils / Q000378:metabolism; D011126:Polypropylenes; D011208:Powders; D011786:Quality Control
D000473:Alkanes; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D000069463:Olive Oil; D010938:Plant Oils; D011126:Polypropylenes; D011208:Powders; D002244:Carbon; D008049:Lipase; C007932:n-hexadecane