title: Involvement of oxidative stress in the regulation of H(2)S production during ultradian metabolic oscillation of Saccharomyces cerevisiae.
abstract: Periodic evolution of H(2)S during aerobic chemostat culture of Saccharomyces cerevisiae resulted in ultradian metabolic oscillation via periodic inhibition of respiratory activity. To understand the nature of periodic H(2)S evolution, we investigated whether oxidative stress is associated with H(2)S production. The cellular oxidative states represented by intracellular level of lipid peroxides oscillated out of phase with the oscillation of dissolved O(2). Pulse addition of antioxidant, oxidative agent or inhibitor of antioxidation enzymes perturbed metabolic oscillation producing changes in H(2)S evolution. Analysis of H(2)S production profiles during perturbation of oscillation revealed that the amount of H(2)S production is closely linked with cellular oxidative states. Based on these results and our previous reports, we suggest that oxidative stresses result in periodic depletion of glutathione and cysteine, which in turn causes stimulation of the sulfate assimilation pathway and H(2)S production.
authors.affiliation: SK chemicals Ltd., 440-745, Suwon, South Korea.
authors.forename: Wie Jong
authors.initials: WJ
authors.lastname: Kwak
authors.forename: Gi Seok
authors.initials: GS
authors.lastname: Kwon
authors.forename: Ingnyol
authors.initials: I
authors.lastname: Jin
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kuriyama
authors.forename: Ho Yong
authors.initials: HY
authors.lastname: Sohn
mesh_terms: D000332:Aerobiosis; D001683:Biological Clocks / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D005978:Glutathione / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*
chemical_list: D005978:Glutathione; D006862:Hydrogen Sulfide