Transposition of the Drosophila hydei Minos transposon in the mouse germ line.
We tested the suitability of the fly transposon Minos, a member of the Tc1/mariner superfamily, for insertional mutagenesis in the mouse germ line. We generated a transgenic mouse line expressing Minos transposase in growing oocytes and another carrying a tandem array of nonautonomous transposons. The frequency of transposition in the progeny derived from oocytes carrying both transgenes is 8.2%. Analysis of the new integration sites shows a high frequency of transpositions to a different chromosome. Thus Minos transposition could be an effective system for insertional mutagenesis and functional genomic analysis in the mouse.
Drabek
Dubravka
D
Department of Cell Biology and Genetics, Faculty of Medicine, Erasmus University Rotterdam, The Netherlands.
Zagoraiou
Laskaro
L
deWit
Ton
T
Langeveld
An
A
Roumpaki
Chariklea
C
Mamalaki
Clio
C
Savakis
Charalambos
C
Grosveld
Frank
F
D000818:Animals; D015139:Blotting, Southern; D004251:DNA Transposable Elements; D004330:Drosophila / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D051379:Mice
D004251:DNA Transposable Elements