NF-kappaB dependent activation of human endothelial cells treated with soluble products derived from human lymphomas.
Nuclear factor of the Immunoglobulin Kappa chain of B cells (NF-kappaB) activation is an early event during cytokine-mediated endothelial activation related to increased adhesion of leucocytes. We report that soluble products secreted by two human lymphomas activate NF-kappaB, and increase the ability of endothelial cells to adhere U937 cells in vitro. Analysis of the tumor-derived products revealed the absence of tumor necrosis factor-alpha and interleukin-1beta. Interference of NF-kappaB activation prevented the increase in U937 cell adhesion, suggesting a potential role for endothelial NF-kappaB activation in the establishment of physical interactions between the vascular endothelium and tumor cells.
Estrada-Bernal
Adriana
A
Departamento de Biología Celular del Instituto de Fisiología Celular UNAM, Copilco Universidad, PO Box 70-243, Mexico DF 04510, Mexico. aestrada@ifisiol.unam.mx
Mendoza-Milla
Criselda
C
Ventura-Gallegos
José Luis
JL
López-Bojórquez
Lucia Nikolaia
LN
Miranda-Peralta
Enrique
E
Arechavaleta-Velasco
Fabián
F
Vadillo-Ortega
Felipe
F
Sánchez-Sánchez
Luis
L
Zentella-Dehesa
Alejandro
A
D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000378:metabolism; D008223:Lymphoma / Q000378:metabolism* / Q000473:pathology; D016328:NF-kappa B / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D020298:U937 Cells
D007375:Interleukin-1; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha