title: [The expression of FN-IIIcs in the wound healing and its relationship with injury time].
abstract: OBJECTIVE
  To research the expression of alternative splicing segment of fibronectin-IIIcs in incised wound of skin, to offer the experimental data for early injury time judgement.
  
  
  METHODS
  Using in situ hybridization with DIG-labeled anti-sense RNA probe, the expression of FN-IIIcs domain was detected in both human and rat skin incised wound.
  
  
  RESULTS
  (1) The expression of FN-IIIcs improved from the time of 30 min after injury, reached peak at 6 h and then reduced gradually. (2) The positive expression cells were mainly distributed in hair follicles, sebaceous glands and endothelium, however, in human beings they were basement cells of epidermis.
  
  
  CONCLUSIONS
  The expression of FN-IIIcs domain would be a supposed useful criteria for early injury time judgement.
authors.affiliation: Department of Forensic Medicine of Fudan University, Shanghai 200032.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ji
authors.forename: Zi-qin
authors.initials: ZQ
authors.lastname: Zhao
authors.forename: Yun-ju
authors.initials: YJ
authors.lastname: Gu
mesh_terms: D000818:Animals; D005353:Fibronectins / Q000096:biosynthesis* / Q000235:genetics; D005554:Forensic Medicine; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries* / Q000378:metabolism*; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*
chemical_list: D005353:Fibronectins; D012333:RNA, Messenger