title: Protein database, human retinal pigment epithelium.
abstract: The retinal pigment epithelium (RPE) is a single cell layer adjacent to the rod and cone photoreceptors that plays key roles in retinal physiology and the biochemistry of vision. RPE cells were isolated from normal adult human donor eyes, subcellular fractions were prepared, and proteins were fractionated by electrophoresis. Following in-gel proteolysis, proteins were identified by peptide sequencing using liquid chromatography tandem electrospray mass spectrometry and/or by peptide mass mapping using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Preliminary analyses have identified 278 proteins and provide a starting point for building a database of the human RPE proteome.
authors.affiliation: Cole Eye Institute and Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
authors.forename: Karen A
authors.initials: KA
authors.lastname: West
authors.forename: Lin
authors.initials: L
authors.lastname: Yan
authors.forename: Karen
authors.initials: K
authors.lastname: Shadrach
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Azeem
authors.initials: A
authors.lastname: Hasan
authors.forename: Masaru
authors.initials: M
authors.lastname: Miyagi
authors.forename: John S
authors.initials: JS
authors.lastname: Crabb
authors.forename: Joe G
authors.initials: JG
authors.lastname: Hollyfield
authors.forename: Alan D
authors.initials: AD
authors.lastname: Marmorstein
authors.forename: John W
authors.initials: JW
authors.lastname: Crabb
mesh_terms: D002853:Chromatography, Liquid; D003600:Cytosol / Q000378:metabolism; D019992:Databases as Topic; D006801:Humans; D008861:Microsomes / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D010857:Pigment Epithelium of Eye / Q000737:chemistry*; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013347:Subcellular Fractions
chemical_list: D010455:Peptides; D020543:Proteome