The degenerative and regenerative processes after the elimination of the proliferative peripheral retina of fish.
We have analyzed the modifications in the tench (Tinca tinca) retina after the complete cryo-elimination of the proliferative growing zone (PGZ), which participates in the continuous growth of the retina throughout the life of the fish. By using immunohistochemistry and electron microscopy we demonstrated that, after the lesion, degenerative and regenerative processes take place in the PGZ, in the ciliary zone, and in the transition zone located between the PGZ and the central retina. After 120 days postlesion, the PGZ was completely regenerated and its composition was similar to that of the control animals. Numerous proliferative PCNA-positive cells reappeared and new ganglion cells were formed. In the transition zone and the central retina numerous proliferative PCNA-positive cells also appeared. These are arranged, on occasion, as columnar units from the inner to the outer nuclear layer where the rod precursors and the progenitor cells, respectively, were located. The Müller cells, closely associated with these columnar units, appeared to use them as guides to migration during the regenerative process. Notably, modifications occurred in the ciliary zone, whose cells acquired similar characteristics to the PGZ cells. The ciliary zone cells, the Müller cells, the rod precursors, and the proliferative cells located in the inner nuclear layer appear to participate actively in the regeneration of the PGZ.
Jimeno
David
D
Biología Celular, INCyL, Universidad de Salamanca, Salamanca, Spain
Lillo
Concepción
C
Cid
Elena
E
Aijón
José
J
Velasco
Almudena
A
Lara
Juan M
JM
D000818:Animals; D002455:Cell Division / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D003452:Cryosurgery; D003530:Cyprinidae / Q000502:physiology*; D008854:Microscopy, Electron; D009410:Nerve Degeneration; D009416:Nerve Regeneration / Q000502:physiology*; D009457:Neuroglia / Q000378:metabolism / Q000648:ultrastructure; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D012160:Retina / Q000254:growth & development / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure*; D012165:Retinal Ganglion Cells / Q000378:metabolism / Q000648:ultrastructure
D018809:Proliferating Cell Nuclear Antigen