Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations.
The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution, distance range, and sensitivity and a broader range of biological applications.
Sekar
Rajesh Babu
RB
W.M. Keck Center for Cellular Imaging, Department of Biology, Gilmer Hall (064), University of Virginia, Charlottesville, VA 22904, USA.
Periasamy
Ammasi
A
D000818:Animals; D005057:Eukaryotic Cells / Q000166:cytology* / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods* / Q000639:trends; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods / Q000639:trends; D008164:Luminescent Proteins; D008856:Microscopy, Fluorescence / Q000379:methods* / Q000639:trends; D011506:Proteins / Q000378:metabolism*
D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D011506:Proteins; D049452:Green Fluorescent Proteins