Cloning, mapping, and developmental expression of a sixth zebrafish Na,K-ATPase alpha1 subunit gene (atp1a1a.5).
We have identified and characterized cDNAs encoding a novel zebrafish Na,K-ATPase alpha subunit. The full-length cDNA encodes a 1,023-amino-acid-long peptide which shows greatest homology to zebrafish alpha1 polypeptides. Radiation hybrid mapping localized the new gene (atp1a1a.5) to linkage group 1 in close proximity to the previously identified cluster of Na,K-ATPase alpha1 genes. The expression of atp1a1a.5 in zebrafish embryos was analyzed using whole-mount in situ hybridization. From mid-somitogenesis through 48 h post fertilization (hpf), atp1a1a.5 transcripts were detected in the pronephric duct, ear, and mucous cells. This expression pattern continues through 108 hpf, when high levels of expression were also detected in the intestinal bulb.
Blasiole
Brian
B
Department of Pharmacology, Penn State College of Medicine, Hershey, PA 17033, USA.
Canfield
Victor
V
Degrave
Agnes
A
Thisse
Christine
C
Thisse
Bernard
B
Rajarao
Johannes
J
Levenson
Robert
R
D000818:Animals; D002874:Chromosome Mapping; D020869:Gene Expression Profiling; D007668:Kidney / Q000196:embryology / Q000378:metabolism; D009928:Organ Specificity; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*; D029961:Zebrafish Proteins
D029961:Zebrafish Proteins; C439245:atp1a1a.1 protein, zebrafish; D000254:Sodium-Potassium-Exchanging ATPase