title: Transcriptional profiling identifies Id2 function in dendritic cell development.
abstract: Dendritic cells (DCs) are potent antigen-presenting cells with a pivotal role in antigen-specific immune responses. Here, we found that the helix-loop-helix transcription factor Id2 is up-regulated during DC development in vitro and crucial for the development of distinct DC subsets in vivo. Id2-/- mice lack Langerhans cells (LCs), the cutaneous contingent of DCs, and the splenic CD8alpha+ DC subset is markedly reduced. Mice deficient for transforming growth factor (TGF)-beta also lack LCs, and we demonstrate here that, in DCs, TGF-beta induces Id2 expression. We also show that Id2 represses B cell genes in DCs. These findings reveal a TGF-beta-Id2 signaling pathway in DCs and suggest a mechanism by which Id2 affects the lineage choice of B cell and DC progenitors.
authors.affiliation: Max-Delbrück-Center for Molecular Medicine, MDC, Robert-Rössle-Str. 10, 13092 Berlin, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Hacker
authors.forename: Ralf D
authors.initials: RD
authors.lastname: Kirsch
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Ju
authors.forename: Thomas
authors.initials: T
authors.lastname: Hieronymus
authors.forename: Tatjana C
authors.initials: TC
authors.lastname: Gust
authors.forename: Christiane
authors.initials: C
authors.lastname: Kuhl
authors.forename: Thorsten
authors.initials: T
authors.lastname: Jorgas
authors.forename: Steffen M
authors.initials: SM
authors.lastname: Kurz
authors.forename: Stefan
authors.initials: S
authors.lastname: Rose-John
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Yokota
authors.forename: Martin
authors.initials: M
authors.lastname: Zenke
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D051797:Inhibitor of Differentiation Protein 2; D020411:Oligonucleotide Array Sequence Analysis; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D019948:Receptors, Interleukin-4 / Q000378:metabolism; D012097:Repressor Proteins; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D004268:DNA-Binding Proteins; C497450:ID2 protein, human; D051797:Inhibitor of Differentiation Protein 2; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D019948:Receptors, Interleukin-4; D012097:Repressor Proteins; D014157:Transcription Factors; D016212:Transforming Growth Factor beta