Interaction of NF-Y with the 3'-flanking DNA sequence of the CCAAT box.
NF-Y, also referred to as CCAAT-binding factor, is a major CCAAT-binding transcription factor. The present study demonstrated that the 3'-flanking region of the CCAAT box is involved in the formation of a stable NF-Y.DNA complex. An electrophoretic mobility shift assay showed that the interaction of NF-Y with DNA 15 bp downstream of the CCAAT box alters not only the affinity of NF-Y for its binding site but also the electrophoretic mobility of the NF-Y.DNA complex. This interaction is accompanied by a conformational change of NF-Y as demonstrated by a change in the reactivity of an anti-NF-YA antibody to the NF-Y.DNA complex.
Sugiura
Naoaki
N
Department of Biochemistry I, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan.
Takishima
Kunio
K
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D023081:CCAAT-Binding Factor / Q000235:genetics / Q000378:metabolism*; D016466:CHO Cells; D006224:Cricetinae; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014407:Tumor Cells, Cultured
D023081:CCAAT-Binding Factor; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; D014157:Transcription Factors; D004247:DNA