[PGM1 genotyping by PCR-SSCP].
OBJECTIVE
PGM1 genotyping by PCR-SSCP analysis.


METHODS
Amplified genome DNA from 156 unrelated Han individuals living in Wuhan, PCR products for exon 4 and exon 8 of PGM1 were then analyzed by SSCP to detect the genotypes.


RESULTS
2 alleles and 3 genotypes were detected in exon 4 and 8 respectively. The discrimination power was 0.7318. PCR-SSCP analysis was suitable for determination of PGM1 genotypes from old blood and semen stains.


CONCLUSIONS
PGM1 system typed by PCR-SSCP is useful for forensic identification.
Song
Hai-yan
HY
Department of Forensic Medicine, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030.
Yang
Qing-en
QE
Yu
Chun-ying
CY
D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002681:China; D004247:DNA / Q000032:analysis / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D010733:Phosphoglucomutase / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D012680:Sensitivity and Specificity
D004247:DNA; D010733:Phosphoglucomutase