title: CD23 and CD69 expression on human neutrophils of healthy subjects and patients with peripheral arterial occlusive disease.
abstract: In this work we studied, on human neutrophils from healthy donors and patients with peripheral arterial occlusive disease, the expression of CD23 and CD69 and the modulatory effects of IFN-gamma, GM-CSF and IL-4. Neutrophils were isolated from 9 patients and 9 healthy subjects and cultured for 24 h in absence or presence of IFN-gamma (1000 U/ml), GM-CSF (10 U/ml) and IL-4 (10 ng/ml). Expression of CD23 and CD69 was analyzed by FACScan cytofluorimeter. Neutrophils of both patients and healthy donors resulted negative for CD23 and CD69 expression immediately after isolation. After 24 h without stimuli, neutrophils from some patients and healthy donors expressed CD23 and CD69. IFN-gamma and GM-CSF had opposite effects on these two antigens, down-regulating CD23 and up-regulating CD69. IFN-gamma, GM-CSF and IL-4 were not able to induce CD23 expression, while CD69 expression was induced in some negative healthy donors and patients by IFN-gamma, GM-CSF and IL-4 respectively. From our data, we identified two subpopulations of neutrophils that, independently from the vascular pathology, showed a different behaviour towards temperature and some cytokines.
authors.affiliation: Dept. Medicine and Public Health, Pharmacology Section, University of Verona, Verona, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Benoni
authors.forename: A
authors.initials: A
authors.lastname: Adami
authors.forename: A
authors.initials: A
authors.lastname: Vella
authors.forename: E
authors.initials: E
authors.lastname: Ariosto
authors.forename: R
authors.initials: R
authors.lastname: Ortolani
authors.forename: L
authors.initials: L
authors.lastname: Cuzzolin