title: Survival of biological cells deformed in a narrow gap.
abstract: Recent studies show that during slow freezing of biological cells, the cells may be also injured by not only chemical damage but also mechanical damage induced by ice crystal compression. A new experimental procedure is developed to quantify cell destruction by deformation with two parallel surfaces. The viability of cells (prostatic carcinoma cells, 17.5 microns in mean diameter) is measured as a function of gap size ranging from 3.5 microns to 16.2 microns at 0 degree C, 23 degrees C and 37 degrees C. The viability at a smaller gap size is significantly lower at 37 degrees C than at 23 degrees C, while the difference between 0 degree C and 23 degrees C is much smaller. This suggests that deformation damage is related to the deformation of the cytoskeleton rather than the mechanical properties of the lipid membrane.
authors.affiliation: Institute of Advanced Material Study, Kyushu University, 6-1 Kasugakoen, Kasuga 816-8580, Japan. takamatu@cm.kyushu-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takamatsu
authors.forename: Norihiko
authors.initials: N
authors.lastname: Kumagae
mesh_terms: D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000473:pathology / Q000503:physiopathology; D048429:Cell Size; D002470:Cell Survival; D003080:Cold Temperature; D005615:Freezing; D006801:Humans; D007038:Hypotonic Solutions; D008297:Male; D009997:Osmotic Pressure; D011471:Prostatic Neoplasms / Q000473:pathology* / Q000503:physiopathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013223:Statistics as Topic; D013314:Stress, Mechanical; D013696:Temperature; D014407:Tumor Cells, Cultured
chemical_list: D007038:Hypotonic Solutions