Peptide G protein agonists from a phage display library.
G proteins may serve as targets for pharmacological activation of signaling pathways bypassing the regulatory events that may counteract the effect of the external stimulus on the level of receptors. We sought to identify peptides as lead structures interacting with G proteins utilizing a commercially available phage-display library. The heptapeptide library was screened for binding to human G alpha(i1) which was modified with a hexahistidine tag and immobilized on a Ni(2+)-coated surface. After several rounds of phage selection a number of phage clones with consensus sequences were found. Peptides with such sequences were chemically synthesized and their effect on [35S]GTP gamma S binding to G alpha(i1) and other G protein alpha subunits was determined. Through this process two peptide 'families' with the capability to activate G proteins were identified. The peptides showed no structural similarity to known peptide or nonpeptide G protein activators with a cationic amphiphilic structure like mastoparan or alkylamines. The functional relevance of the peptide-G protein interaction was shown by an increased sensitivity for guanine nucleotides of high-affinity agonist binding to A(1) adenosine receptors. The peptide G protein activators may, therefore, serve as novel tools for further investigation of receptor-independent activation of G proteins.
Hessling
Jutta
J
Institut für Pharmakologie und Toxikologie, Universität Würzburg, Versbacher Str. 9, D-97078 Würzburg, Germany.
Lohse
Martin J
MJ
Klotz
Karl-Norbert
KN
D000818:Animals; D001665:Binding Sites; D016466:CHO Cells; D006224:Cricetinae; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism; D015398:Signal Transduction
D019151:Peptide Library; D010455:Peptides; D020962:Heterotrimeric GTP-Binding Proteins