Universal primers for the PCR-mediated amplification of DNA 1: a satellite-like molecule associated with begomovirus-DNA beta complexes.
DNA 1 is a single-stranded DNA molecule of approximately 1370 nucleotides. It is associated with monopartite geminiviruses of the genus Begomovirus, which require a DNA beta component for symptomatic infection. The DNA 1 molecule requires the helper begomovirus for movement in plants, but is capable of self-replication. We designed two abutting primer pairs (DNA101/DNA102 and UN101/UN102) to conserved sequences of DNA 1. This allowed polymerase chain reaction-mediated amplification of the full-length molecule from total nucleic acid extracts produced from various host plants from geographically distinct, worldwide locations. These primers are useful both as diagnostic probes and for producing full-length infectious clones for in planta studies.
Bull
S E
SE
Department of Disease and Stress Biology, John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK. simon.bull@bbsrc.ac.uk
Briddon
R W
RW
Markham
P G
PG
D031163:Ageratum / Q000821:virology; D001483:Base Sequence; D017931:DNA Primers; D004276:DNA, Satellite / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D017791:Geminiviridae / Q000235:genetics*; D003368:Gossypium / Q000821:virology; D018551:Solanum lycopersicum / Q000821:virology; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D004276:DNA, Satellite; D004279:DNA, Viral