title: Possible quorum sensing in the rumen microbial community: detection of quorum-sensing signal molecules from rumen bacteria.
abstract: The bioluminescence assay using Vibrio harveyi BB170 was used to examine quorum-sensing autoinducer 2 (AI-2) activity from cell-free culture fluids of rumen bacteria. The assay showed that the culture fluids of four species of rumen bacteria, Butyrivibrio fibrisolvens, Eubacterium ruminantium, Ruminococcus flavefaciens, and Succinimonas amylolytica, contained AI-2-like molecules. Furthermore, homologues for luxS genes were detected in rumen fluids collected from three cows and in bacterial cells of P. ruminicola subsp. ruminicola and R. flavefaciens. These findings suggest that the quorum-sensing system mediated by AI-2 is present in the rumen.
authors.affiliation: National Institute of Livestock and Grassland Science, Ikenodai 2, Tsukuba, 305-0901, Ibaraki, Japan. mitumori@affrc.go.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Mitsumori
authors.forename: Liming
authors.initials: L
authors.lastname: Xu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kajikawa
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Kurihara
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Tajima
authors.forename: Jin
authors.initials: J
authors.lastname: Hai
authors.forename: Akio
authors.initials: A
authors.lastname: Takenaka
mesh_terms: D000818:Animals; D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D013437:Carbon-Sulfur Lyases; D002417:Cattle; D017077:Culture Media, Conditioned; D015964:Gene Expression Regulation, Bacterial; D006714:Homoserine / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism*; D007783:Lactones / Q000378:metabolism*; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D010802:Phylogeny; D012417:Rumen / Q000382:microbiology; D017422:Sequence Analysis, DNA; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; D017077:Culture Media, Conditioned; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D006714:Homoserine; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria