Development, characterization, and validation of a sensitive primate-specific quantification assay for forensic analysis.
Accurate human-specific DNA quantification is essential for forensic casework analysis. In this work, we describe a microplate-based quantification assay that utilizes the PCR amplification of human-specific TH01 primers. This method enables the reliable quantification of human DNA samples from 0.2 to 40 ng, even in mixtures with nonhuman DNA. Analysis of samples can be semi-automated using 96-well microplates and a spreadsheet-based concentration calculator for high-throughput demands. We have used this quantification method with more than 15,000 forensic samples.
Fox
John C
JC
Bode Technology Group, Springfield, VA, USA.
Cave
Christopher A
CA
Schumm
James W
JW
D000818:Animals; D004247:DNA / Q000032:analysis* / Q000145:classification* / Q000235:genetics; D005470:Fluorometry / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D008253:Macaca mulatta; D008832:Microchemistry / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D011323:Primates / Q000235:genetics*; D011786:Quality Control; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012984:Software; D013045:Species Specificity
D004247:DNA