title: Evidence for glycosylphosphatidylinositol (GPI)-anchored eosinophil-derived neurotoxin (EDN) on human granulocytes.
abstract: Eosinophil-derived neurotoxin (EDN) is one of the four basic proteins stored in specific eosinophil granules. Here we demonstrate that EDN can also be detected at the surface of granulocytes. Reduction of EDN membrane expression after phosphatidylinositol-specific phospholipase C treatment suggests that a glycosylphosphatidylinositol (GPI) anchor is involved in the membrane association of EDN. The presence of a GPI anchor was confirmed by a lower expression of membrane EDN on granulocytes from patients with paroxysmal nocturnal hemoglobinuria which present cells lacking GPI anchor proteins. Furthermore, metabolic labeling with GPI anchor components supports biochemical evidence of GPI anchoring of EDN.
authors.affiliation: Institute for Virology, Medical Center for Hygiene, Philipps University, D-35037 Marburg, Germany. debierre@mailer.uni-marburg.de
authors.forename: Françoise
authors.initials: F
authors.lastname: Debierre-Grockiego
authors.forename: Corinne
authors.initials: C
authors.lastname: Desaint
authors.forename: Vincent
authors.initials: V
authors.lastname: Fuentes
authors.forename: Mathilde
authors.initials: M
authors.lastname: Poussin
authors.forename: Gérard
authors.initials: G
authors.lastname: Socié
authors.forename: Nahid
authors.initials: N
authors.lastname: Azzouz
authors.forename: Ralph T
authors.initials: RT
authors.lastname: Schwarz
authors.forename: Lionel
authors.initials: L
authors.lastname: Prin
authors.forename: Valérie
authors.initials: V
authors.lastname: Gouilleux-Gruart
mesh_terms: D001782:Blood Donors; D003594:Cytoplasmic Granules / Q000737:chemistry; D047208:Eosinophil-Derived Neurotoxin; D004802:Eosinophilia / Q000097:blood; D004804:Eosinophils / Q000737:chemistry; D005434:Flow Cytometry; D017261:Glycosylphosphatidylinositols / Q000502:physiology*; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood; D006801:Humans; D007958:Leukocyte Count; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D012016:Reference Values; D012260:Ribonucleases / Q000097:blood / Q000633:toxicity*
chemical_list: D017261:Glycosylphosphatidylinositols; D047208:Eosinophil-Derived Neurotoxin; D012260:Ribonucleases