Elucidation of signal transduction pathways by transfection of cells with modified oncogenes.
This chapter will focus on introduction of various wild type (WT) and mutant genes into cells by DNA transfection. Techniques for analysis of the inheritance, expression, and biological effects of the introduced genes will be described. Various strong and weak points about three different techniques of stable gene transfer, including calcium-phosphate DNA precipitation, transfection via liposomes, and transfection via electroporation, will be discussed.
Steelman
Linda S
LS
Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC, USA.
Blalock
William L
WL
Wang
Xiao-Yang
XY
Moye
Phillip W
PW
Lee
John T
JT
Shelton
John G
JG
Navolanic
Patrick M
PM
Davis
Julianne M
JM
Knapp
Steven L
SL
Franklin
Richard A
RA
White
Martyn K
MK
McCubrey
James A
JA
D001940:Breast / Q000166:cytology; D001943:Breast Neoplasms / Q000235:genetics; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009857:Oncogenes / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D014162:Transfection / Q000379:methods*