title: [The incidence of human papilloma virus (HPV) DNA in patients with cervical carcinoma from Gdansk region].
abstract: OBJECTIVE
  We have estimated the incidence of human papilloma virus (HPV) DNA in cervical smears of patients with cervical carcinoma, treated in 1997-1998 at the II Department of Obstetrics and Gynecology, Medical University of Gdańsk, Poland.
  
  
  METHODS
  We have examined 107 patients suffering from cervical cancer. The HPV DNA was detected with the polymerase chain reaction (PCR) method using two primer pairs located in the E6, E7 open reading frames. HPV typing was executed by restriction fragments length polymorphism PCR (RFLP-PCR) method.
  
  
  RESULTS
  HPV DNA was detected in 75 (70.1%) patients. A group of patients with Ib (43%) and IIa (19.6%) clinical degrees made the highest percentage within the research group. We found no statistically significant differences between various clinical degrees of cervical cancer in the group of patients both HPV positive and negative.
  
  
  CONCLUSIONS
  The frequency of HPV especially HPV 16 type infection incidence in cervical carcinoma is lower in Gdansk region than currently published.
authors.affiliation: Samodzielna Pracownia Endokrynologii i Diagnostyki Laboratoryjnej, Gdansk.
authors.forename: Joanna
authors.initials: J
authors.lastname: Liss
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Łukaszuk
authors.forename: Jacek
authors.initials: J
authors.lastname: Gulczyński
authors.forename: Mariusz
authors.initials: M
authors.lastname: Zwaliński
authors.forename: Izabela
authors.initials: I
authors.lastname: Woźniak
authors.forename: Janusz
authors.initials: J
authors.lastname: Emerich
authors.forename: Czesław
authors.initials: C
authors.lastname: Wójcikowski
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D015994:Incidence; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D014412:Tumor Virus Infections / Q000453:epidemiology* / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology* / Q000821:virology*; D014626:Vaginal Smears
chemical_list: D004279:DNA, Viral