title: Presence of endometrial epithelial cells in the peritoneal cavity and the mesothelial inflammatory response.
abstract: OBJECTIVE
  To determine the contribution of endometrial cells in the development of endometriosis. Specifically the response of the mesothelium to endometrial cells in the production of monocyte chemotactic protein-1 (MCP-1), interleukin-6 (IL-6), and IL-8 was studied.
  
  
  METHODS
  In vitro study.
  
  
  METHODS
  University Research Laboratory.
  
  
  METHODS
  None.
  
  
  METHODS
  None.
  
  
  METHODS
  Cellular MCP-1, IL-6 secretion and MCP-1, and IL-6 and IL-8 messenger RNA expression were evaluated by ELISA and reverse transcription-polymerase chain reaction (RT-PCR) assay.
  
  
  RESULTS
  The mesothelial cells produced more MCP-1 and IL-6 than endometrial epithelial and stromal cells. Mesothelial cells cultured in the presence of endometrial epithelial cells produced even greater levels of MCP-1 and IL-6 than those cultured in the presence of stromal cells or cultured alone. The MCP-1, IL-6, and IL-8 mRNA expression also increased when mesothelial cells were co-cultured with endometrial epithelial cells.
  
  
  CONCLUSIONS
  The results suggest that endometrial epithelial cells may be important in evoking the inflammatory reaction in the peritoneal cavity during retrograde menstruation and that mesothelial cells may play an important role in the chemotaxis of monocytes and in the inflammatory process during the development of endometriosis.
authors.affiliation: Emory Center for Advanced Research on Women's Health, Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
authors.forename: Mingqing
authors.initials: M
authors.lastname: Song
authors.forename: Sonia A
authors.initials: SA
authors.lastname: Karabina
authors.forename: Nino
authors.initials: N
authors.lastname: Kavtaradze
authors.forename: Ana A
authors.initials: AA
authors.lastname: Murphy
authors.forename: Sampath
authors.initials: S
authors.lastname: Parthasarathy
mesh_terms: D018932:Chemokine CCL2 / Q000096:biosynthesis / Q000235:genetics; D018920:Coculture Techniques; D004715:Endometriosis / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics; D010537:Peritoneum / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018932:Chemokine CCL2; D015850:Interleukin-6; D016209:Interleukin-8; D012313:RNA