title: Isoflavone aglycon produced by culture of soybean extracts with basidiomycetes and its anti-angiogenic activity.
abstract: Soybean extracts (SBE) containing isoflavone glycosides were cultured with Ganoderma lucidum mycelia producing beta-glucosidase. The anti-angiogenic effects of the cultivated product, containing rich in genistein, named GCP (genistein combined polysaccharide), were assessed with chick chorioallantoic membranes (CAM) and a mouse dorsal air-sac model. Beta-glucosidase produced by the mycelia converted the isoflavone glycosides into aglycons. A test of volunteers showed that serum concentrations of genistein in the subjects treated with GCP (n = 4) at 3 h after administration were significantly higher than those in the subjects treated with SBE (n = 4). GCP inhibited angiogenesis in CAM, and the activity of GCP was greater than that of SBE. GCP inhibited the formation of new vessels induced by colon carcinoma cells in vivo.
authors.affiliation: Department of Biochemistry, Amino Up Chemical Co., Ltd., 363-32 Shin-ei, Kiyota-ku, Sapporo 004-0839, Japan. au_labo@aminoup.co.jp
authors.forename: Takehito
authors.initials: T
authors.lastname: Miura
authors.forename: Lan
authors.initials: L
authors.lastname: Yuan
authors.forename: Buxiang
authors.initials: B
authors.lastname: Sun
authors.forename: Hajime
authors.initials: H
authors.lastname: Fujii
authors.forename: Mayumi
authors.initials: M
authors.lastname: Yoshida
authors.forename: Koji
authors.initials: K
authors.lastname: Wakame
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Kosuna
mesh_terms: D000328:Adult; D000400:Air Sacs / Q000098:blood supply / Q000187:drug effects; D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D002642:Chick Embryo; D003110:Colonic Neoplasms / Q000098:blood supply / Q000188:drug therapy; D005260:Female; D019833:Genistein / Q000097:blood / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology* / Q000627:therapeutic use; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D020940:Reishi / Q000737:chemistry* / Q000201:enzymology; D013025:Glycine max / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; D007529:Isoflavones; D010936:Plant Extracts; D011134:Polysaccharides; C473311:genistein combined polysaccharide; D019833:Genistein; D001617:beta-Glucosidase