title: Antineutrophil cytoplasmic antibodies directed against bactericidal/permeability-increasing protein detected in children with cystic fibrosis inhibit neutrophil-mediated killing of Pseudomonas aeruginosa.
abstract: Antineutrophil cytoplasmic antibodies (ANCA) directed against bactericidal/permeability-increasing protein (BPI) were repeatedly found in cystic fibrosis (CF) patients. We analyzed the effect of BPI-ANCA in inhibiting neutrophil-mediated killing of Pseudomonas aeruginosa. The bactericidal effect expressed as percentage of killed bacteria after 1 h incubation with neutrophils was 55% when the neutrophils were pretreated with normal human serum, ranged from 49 to 63% with the sera from control BPI-ANCA-negative groups and sharply decreased to the mean 30.5% (range 8-51%) in the presence of BPI-ANCA. Furthermore, the effect mediated by BPI-ANCA was dose dependent and reflected the titer of BPI-ANCA in tested sera.
authors.affiliation: Institute of Immunology, University Hospital Motol, V Uvalu 84 150 06, 5, Praha, Czech Republic. anna.sediva@lfmotol.cuni.cz
authors.forename: Anna
authors.initials: A
authors.lastname: Sedivá
authors.forename: Jirina
authors.initials: J
authors.lastname: Bartůnková
authors.forename: Jana
authors.initials: J
authors.lastname: Bartosová
authors.forename: Charles
authors.initials: C
authors.lastname: Jennette
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Falk
authors.forename: Hitendra S
authors.initials: HS
authors.lastname: Jethwa
mesh_terms: D000328:Adult; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood / Q000276:immunology* / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins / Q000276:immunology*; D002648:Child; D003550:Cystic Fibrosis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008565:Membrane Proteins; D018375:Neutrophil Activation; D009504:Neutrophils / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology / Q000378:metabolism
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D008565:Membrane Proteins; C021623:bactericidal permeability increasing protein