title: Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations.
abstract: The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution, distance range, and sensitivity and a broader range of biological applications.
authors.affiliation: W.M. Keck Center for Cellular Imaging, Department of Biology, Gilmer Hall (064), University of Virginia, Charlottesville, VA 22904, USA.
authors.forename: Rajesh Babu
authors.initials: RB
authors.lastname: Sekar
authors.forename: Ammasi
authors.initials: A
authors.lastname: Periasamy
mesh_terms: D000818:Animals; D005057:Eukaryotic Cells / Q000166:cytology* / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods* / Q000639:trends; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods / Q000639:trends; D008164:Luminescent Proteins; D008856:Microscopy, Fluorescence / Q000379:methods* / Q000639:trends; D011506:Proteins / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D011506:Proteins; D049452:Green Fluorescent Proteins