Detection of rhinovirus and enterovirus in upper respiratory tract samples using a multiplex nested PCR.
Human enteroviruses and rhinoviruses are respiratory pathogens whose role in acute respiratory infection is underestimated due to the use of diagnostic procedures with poor sensitivity. To determine the prevalence of these two pathogens in the upper respiratory tract infections, a multiplex procedure was developed that both detect and differentiate rhinoviruses and enteroviruses. This sensitive procedure allowed the detection of both pathogens from archival material (nasal swabs) collected during the previous winters and differentiated rhinoviruses from enteroviruses. This procedure can be used to determine the role of these pathogens in mild or severe upper respiratory tract infections.
Billaud
Geneviève
G
CNR Enterovirus et CNR Grippe (France sud), Laboratoire de Virologie des Hospices Civils de Lyon, UMR 5537, Domaine Rockefeller, 69373 Lyon Cedex 08, France.
Peny
Stéphanie
S
Legay
Vincent
V
Lina
Bruno
B
Valette
Martine
M
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D004285:Dogs; D004770:Enterovirus / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D004769:Enterovirus Infections / Q000821:virology; D006801:Humans; D010850:Picornaviridae Infections / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012141:Respiratory Tract Infections / Q000821:virology*; D012229:Rhinovirus / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data
D017931:DNA Primers