title: Modulation of oxidative stress in response to gamma-radiation in human glioma cell lines.
abstract: Radiation therapy is routinely used in the management of primary central nervous system malignancies. However, the efficacy of this therapeutic modality is limited by the occurrence of resistance. In the present study, we investigated whether modulation of oxidative stress might underlie glioma cell radioresistance. Superoxide dismutase activity in irradiated M059J cells was two-fold higher than that in untreated controls, but did not significantly change in U-87 and U-138 cells. This is accompanied by an increase in reactive oxygen species content and decreases in cells viability. Pharmacological or genetic modulation of oxidative stress could be associated with an enhancement in the susceptibility of tumor cells to radiation therapy.
authors.affiliation: Laboratório de Estresse Oxidativo, Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. pizzol@ez-poa.com.br
authors.forename: Felipe
authors.initials: F
authors.lastname: Dal-Pizzol
authors.forename: Cristiane
authors.initials: C
authors.lastname: Ritter
authors.forename: Fábio
authors.initials: F
authors.lastname: Klamt
authors.forename: Michael
authors.initials: M
authors.lastname: Andrades
authors.forename: Mário Luís Conte
authors.initials: ML
authors.lastname: da Frota
authors.forename: Cléber
authors.initials: C
authors.lastname: Diel
authors.forename: Celito
authors.initials: C
authors.lastname: de Lima
authors.forename: Aroldo
authors.initials: A
authors.lastname: Braga Filho
authors.forename: Gilberto
authors.initials: G
authors.lastname: Schwartsmann
authors.forename: José Cláudio Fonseca
authors.initials: JC
authors.lastname: Moreira
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000201:enzymology*; D002374:Catalase / Q000378:metabolism; D002470:Cell Survival; D005609:Free Radicals / Q000378:metabolism; D005720:Gamma Rays; D015870:Gene Expression; D005910:Glioma / Q000201:enzymology*; D018384:Oxidative Stress; D011836:Radiation Tolerance; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000528:radiation effects
chemical_list: D005609:Free Radicals; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D013482:Superoxide Dismutase