title: [Effects of mitogen-activated protein kinase(MAPK) in cell signal transduction of chronic myelogenous leukemia cell line K562].
abstract: OBJECTIVE
  Ras-MAPK signal transduction has been thought to play an important role in the carcinogenesis of chronic myelogenous leukemia. In this study, the authors investigated the effects and mechanism of mitogen-activated protein kinase (MAPK) in cell signal transduction of chronic myelogenous leukemia(CML).
  
  
  METHODS
  After MAPK antisense oligodeoxynucleotide (ASO) was introduced into K562 cell line by liposomal transfection, the effects of ASO on K562 cell were evaluated by cell proliferation, DNA synthesis, MAPK protein content and MAPK activity.
  
  
  RESULTS
  The cell proliferation, DNA synthesis, MAPK protein content and MAPK activity were significantly inhibited by MAPK ASO, the inhibitory rates were 51.8%, 57.1%, 45.3%, and 61.6%, respectively,with significant difference in comparison to the control (P<0.05).
  
  
  CONCLUSIONS
  MAPK plays an important role in the signal transduction of CML and MAPK may become a new target in the treatment of CML.
authors.affiliation: Department of Hematology, Xijing Hospital, Xi'an, Shaanxi, 710032, PR China. shangzc2002@yahoo.com.cn
authors.forename: Zhen-Chuan
authors.initials: ZC
authors.lastname: Shang
authors.forename: Bing-Zhong
authors.initials: BZ
authors.lastname: Sun
authors.forename: Zhi-Nan
authors.initials: ZN
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Feng
authors.forename: Sha
authors.initials: S
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
mesh_terms: D004273:DNA, Neoplasm / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm; D020319:Oligodeoxyribonucleotides, Antisense; D020928:Mitogen-Activated Protein Kinases