title: Decreased expression of catalase mRNA in thyroid anaplastic carcinoma.
abstract: BACKGROUND
  A decreased expression of glutathione peroxidase mRNA, an antioxidant enzyme, was previously observed in thyroid anaplastic carcinomas.
  
  
  METHODS
  To clarify the expression of antioxidant-related enzymes in thyroid anaplastic carcinomas, the expression levels of catalase, copper and zinc superoxide dismutase and manganese superoxide dismutase mRNA in 85 benign and malignant thyroid tissues were measured by means of real-time quantitative reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  Decreased expression levels of catalase and copper and zinc superoxide dismutase mRNAs, but not manganese mRNA, were observed in five anaplastic carcinomas compared with normal thyroid tissues and differentiated tumors.
  
  
  CONCLUSIONS
  These results suggest the possibility that anaplastic carcinoma cells are more likely to suffer damage by oxygen free radicals than normal thyroid cells or differentiated tumor cells.
authors.affiliation: Department of Laboratory Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Hasegawa
authors.forename: Toru
authors.initials: T
authors.lastname: Takano
authors.forename: Akira
authors.initials: A
authors.lastname: Miyauchi
authors.forename: Fumio
authors.initials: F
authors.lastname: Matsuzuka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Kanji
authors.initials: K
authors.lastname: Kuma
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Amino
mesh_terms: D000236:Adenoma / Q000378:metabolism; D002277:Carcinoma / Q000201:enzymology* / Q000378:metabolism; D002291:Carcinoma, Papillary / Q000378:metabolism; D002374:Catalase / Q000096:biosynthesis* / Q000235:genetics; D003300:Copper / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000201:enzymology* / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D003300:Copper; D002374:Catalase; D013482:Superoxide Dismutase; D015032:Zinc