title: Genotype identification of Orientia tsutsugamushi isolated from Nan Peng Lie Islands in China.
abstract: OBJECTIVE
  To identify genotype of eight strains of Orientia tsutsugamushi (O. tsutsugamushi) isolated from Nan Peng Lie Islands in China and establish tsutsugamushi disease nature foci for this region.
  
  
  METHODS
  The nested polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used. Three primers were selected from the DNA sequence of the gene encoding type-specific 56-kDa protein of the Karp strain. The positive products were digested by Hine II and Pst I, meanwhile profiles specific to each strain were analyzed.
  
  
  RESULTS
  Three genotypes of O. tsutsugamushi including Karp, Kato and a new strain existed on Nan Peng Lie Islands.
  
  
  CONCLUSIONS
  Nan Peng Lie Islands is tsutsugamushi disease nature foci.
authors.affiliation: Department of Epidemiology, Medical Institute, Guangzhou Command PLA, Guangzhou 510507, China.
authors.forename: Guifu
authors.initials: G
authors.lastname: Peng
authors.forename: Zhibing
authors.initials: Z
authors.lastname: Wang
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.forename: Jialiang
authors.initials: J
authors.lastname: Huang
authors.forename: Pulin
authors.initials: P
authors.lastname: Jiang
authors.forename: Nianhua
authors.initials: N
authors.lastname: Zeng
authors.forename: Jinhua
authors.initials: J
authors.lastname: Liu
authors.forename: Shaofan
authors.initials: S
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D005838:Genotype; D051379:Mice; D012285:Orientia tsutsugamushi / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats
chemical_list: D004269:DNA, Bacterial