Studies of the mechanism of action of the aerolysin-like hemolysin of Aeromonas sobria in stimulating T84 cells to produce cyclic AMP.
We previously reported that the aerolysin-like hemolysin of Aeromonas sobria stimulates T84 cells to produce cyclic AMP, which then emerges in the culture medium. In order to clarify the mechanism of action of the hemolysin, we examined the involvement of adenosine nucleotide. The results show that the hemolysin stimulates T84 cells to release ATP, which is then converted to adenosine by ectonucleotidase. The adenosine generated might stimulate the P1 adenosine receptors of T84 cells to produce cyclic AMP.
Fujii
Yoshio
Y
Institute of Pharmacognosy, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro, Tokushima, Tokushima 770-8514, Japan. fujii@ph.bunri-u.ac.jp
Nomura
Tomohiko
T
Yokoyama
Ritsuko
R
Shinoda
Sumio
S
Okamoto
Keinosuke
K
D000241:Adenosine / Q000096:biosynthesis; D000243:Adenosine Deaminase / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000502:physiology; D000333:Aeromonas / Q000472:pathogenicity*; D001427:Bacterial Toxins / Q000494:pharmacology*; D002460:Cell Line; D003106:Colon / Q000187:drug effects / Q000378:metabolism; D000242:Cyclic AMP / Q000096:biosynthesis*; D006460:Hemolysin Proteins / Q000494:pharmacology*; D006801:Humans; D052899:Pore Forming Cytotoxic Proteins; D014970:Xanthines / Q000494:pharmacology
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D052899:Pore Forming Cytotoxic Proteins; D014970:Xanthines; C032676:aerolysin; D000255:Adenosine Triphosphate; C051869:8-(4-((2-aminoethyl)aminocarbonylmethyloxy)phenyl)-1,3-dipropylxanthine; D000242:Cyclic AMP; D000243:Adenosine Deaminase; D000241:Adenosine