[The effects of cholecystokinin octapeptide on PKC activity in rat cerebral cortex neurocytes].
OBJECTIVE
To investigate the effects of CCK8 on protein kinase C activity in rat cerebral cortex.


METHODS
The cerebral cortex neurocytes were isolated and used as a model. The effects of CCK8, L-364, 718 and L-365, 260 on PKC activities were detected by using a non-radioactive method.


RESULTS
CCK8 caused a detectable increase in PKC activity at 10(-11) mol/L, and a peak increase of PKC activity was observed at 10(-5) mol/L (about 4.5 U/mg protein). PKC activity was increased in dose-dependent manner by CCK8(10(-11)-10(-6) mol/L). The CCKB-selective receptor antagonist L-365, 260 with a higher efficiency, and the CCKA-selective receptor antagonist L-364, 718 with a lower efficiency were able to block a maximal effect of CCK8-induced PKC activation.


CONCLUSIONS
CCK8 may regulate PKC activities in rat cerebral cortex through CCKB receptor.
Xiang
Peng
P
Lab. of Hormone-receptor, Department of Biochemistry, West China School of Predinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Qiu
Mingjun
M
Du
Zeli
Z
Chen
Manling
M
Wu
Zhaofeng
Z
D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology*; D020109:Devazepide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D009474:Neurons / Q000166:cytology / Q000201:enzymology; D010671:Phenylurea Compounds / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors; D012844:Sincalide / Q000494:pharmacology*
D001570:Benzodiazepinones; D010671:Phenylurea Compounds; D011949:Receptors, Cholecystokinin; C058121:L 365260; D011493:Protein Kinase C; D020109:Devazepide; D012844:Sincalide