Preliminary characterisation of mechanoresponsive regions of the c-fos promoter in bone cells.
Preliminary analysis of the mechanisms involved in induction of stretch-mediated transcriptional activity in the c-fos promoter of bone has been undertaken using a series of c-fos promoter-reporter constructs. UMR-106 osteoblastic cells transfected with reporter constructs were subjected to cyclical physiological loading. The major determinants in the resulting transcriptional mechanoactivation are within the sequence between -356 and -151 which contains the serum response element and a consensus shear stress response element. Elements beyond this region also play a role as deletion of this region does not eliminate mechanoinduction. These results suggest that the mechanical induction of c-fos in osteoblastic bone cells is mediated by multiple response elements.
Peake
Matthew A
MA
Centre for Science and Technology in Medicine, School of Medicine, Keele University, North Staffordshire Hospital, Hartshill, Stoke-on-Trent, UK.
El Haj
Alicia J
AJ
D000818:Animals; D001665:Binding Sites; D001859:Bone Neoplasms; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D017930:Genes, Reporter; D016762:Genes, fos; D010006:Osteoblasts / Q000502:physiology*; D012516:Osteosarcoma; D010957:Plasmids; D011401:Promoter Regions, Genetic; D016760:Proto-Oncogene Proteins c-fos / Q000737:chemistry* / Q000235:genetics*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013314:Stress, Mechanical; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D016760:Proto-Oncogene Proteins c-fos; D011993:Recombinant Fusion Proteins; D015500:Chloramphenicol O-Acetyltransferase