Crystallization and preliminary crystallographic studies of juvenile hormone-binding protein from Galleria mellonella haemolymph.
Juvenile hormone-binding protein (JHBP) isolated from Galleria mellonella haemolymph has been crystallized using the hanging-drop method in two polymorphic forms. The best diffracting crystals (2.7 A) are trigonal, space group P3(1)21 (or P3(2)21), with unit-cell parameters a = 110.4, c = 93.9 A. X-ray diffraction data have been collected for the native crystals using synchrotron radiation and cryogenic conditions (100 K).
Kołodziejczyk
Robert
R
Division of Biochemistry, Institute of Organic Chemistry, Biochemistry and Biotechnology, Wrocław University of Technology, Poland.
Kochman
Marian
M
Bujacz
Grzegorz
G
Dobryszycki
Piotr
P
Ozyhar
Andrzej
A
Jaskolski
Mariusz
M
D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D006458:Hemolymph / Q000737:chemistry*; D007202:Indicators and Reagents; D019476:Insect Proteins; D007915:Lepidoptera / Q000737:chemistry*
D002352:Carrier Proteins; D007202:Indicators and Reagents; D019476:Insect Proteins; C035606:juvenile hormone-binding protein, insect