title: [Effects of veterinary drugs on beta-hexosaminidase release from rat basophilic leukemia cells (RBL-2H3)].
abstract: Little is known about the effects of residual veterinary drugs on the allergic reaction, except for the antigenicity of antibiotics and synthetic antimicrobials. Therefore, 59 kinds of veterinary drugs were investigated for their effects on the IgE receptor-mediated beta-hexosaminidase release from RBL-2H3 cells as an index of immediate allergic reaction. We found that the antibiotics chlorotetracycline, doxycycline, monensin, the synthetic antimicrobial pyrimethamine and the steroid hormone testosterone inhibited beta-hexosaminidase release. Most of the veterinary drugs showed no action, though the ionophores lasalocid, salinomycin and the steroid hormone hexestrol promoted beta-hexosaminidase release from injured cells. Based on the residual levels of these drugs and the frequencies of detection in actual food samples, it seems unlikely that these drugs have any immediate allergic effect in practice.
authors.affiliation: Osaka Prefectural Institute of Public Health: 1-3-69, Nakamichi, Higashinari-ku, Osaka 537-0025, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Shuzo
authors.initials: S
authors.lastname: Taguchi
authors.forename: Seisaku
authors.initials: S
authors.lastname: Yoshida
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Hori
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Takagaki
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002751:Chlortetracycline / Q000494:pharmacology; D004318:Doxycycline; D004350:Drug Residues / Q000494:pharmacology; D006589:Hexestrol / Q000494:pharmacology; D006969:Hypersensitivity, Immediate / Q000139:chemically induced; D007832:Lasalocid / Q000494:pharmacology; D007942:Leukemia, Experimental; D008985:Monensin / Q000494:pharmacology; D011714:Pyrans / Q000494:pharmacology; D011739:Pyrimethamine / Q000494:pharmacology; D051381:Rats; D017455:Receptors, IgE; D013739:Testosterone / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D019155:Veterinary Drugs / Q000494:pharmacology*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism*; D000095662:Polyether Polyketides
chemical_list: D000900:Anti-Bacterial Agents; D002751:Chlortetracycline; D004318:Doxycycline; D006589:Hexestrol; D007832:Lasalocid; D008985:Monensin; D011714:Pyrans; D011739:Pyrimethamine; D017455:Receptors, IgE; D013739:Testosterone; D019155:Veterinary Drugs; D001619:beta-N-Acetylhexosaminidases; C010327:salinomycin; D000095662:Polyether Polyketides