Preparation of a polybutadiene stationary phase immobilized by gamma radiation for reversed-phase high-performance liquid chromatography.
Polybutadiene (PBD) has been immobilized on HPLC silica by gamma radiation doses in the range from 5 to 180 kGy. Columns prepared from these reversed-phase materials, as well as from similar non-irradiated materials, were tested with standard sample mixtures and characterized by elemental analysis (% C) and infrared spectroscopy. A low dose of 5 kGy is sufficient to produce a layer of immobilized PBD which functions as an efficient and stable stationary phase. Higher doses give thicker immobilized layers having less favorable chromatographic properties.
Lopes
Nilva P
NP
LABCROM-Laboratório de Pesquisas em Cromatografia Líquida, Instituto de Química, Universidade Estadual de Campinas, Caixa Postal 6154, CEP 13084-971, Campinas, SP, Brazil.
Collins
Kenneth E
KE
Jardim
Isabel C S F
IC
D002070:Butadienes / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D020724:Elastomers; D005720:Gamma Rays; D011108:Polymers / Q000737:chemistry*; D013055:Spectrophotometry, Infrared
D002070:Butadienes; D020724:Elastomers; D011108:Polymers; C028834:polybutadiene