title: Crystallization and preliminary X-ray crystallographic studies of chorismate synthase from Helicobacter pylori.
abstract: Chorismate synthase (EC 4.6.1.4) catalyzes the transformation of 5-enolpyruvylshikimate 3-phosphate to chorismate in the last step of the shikimate pathway. Chorismate synthase from Helicobacter pylori fused with an eight-residue C-terminal tag was overexpressed in soluble form in Escherichia coli. It was crystallized at 296 K using polyethylene glycol 400 as a precipitant. A set of X-ray diffraction data was collected to 2.5 A resolution using synchrotron radiation. The crystals belong to the tetragonal space group I4, with unit-cell parameters a = b = 145.79, c = 130.98 A. The asymmetric unit contains a tetramer, giving a crystal Volume per protein mass (V(M)) of 2.13 A(3) Da(-1) and a solvent content of 42.3%.
authors.affiliation: Structural Proteomics Laboratory, School of Chemistry and Molecular Engineering, Seoul National University, Seoul 151 742, South Korea.
authors.forename: Hyung Jun
authors.initials: HJ
authors.lastname: Ahn
authors.forename: Jin Kuk
authors.initials: JK
authors.lastname: Yang
authors.forename: Byung Il
authors.initials: BI
authors.lastname: Lee
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Yoon
authors.forename: Hyung Wook
authors.initials: HW
authors.lastname: Kim
authors.forename: Se Won
authors.initials: SW
authors.lastname: Suh
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism; D016480:Helicobacter pylori / Q000201:enzymology*; D008027:Light; D019761:Phosphorus-Oxygen Lyases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D012542:Scattering, Radiation
chemical_list: D018076:DNA, Complementary; C019895:chorismate synthase; D019761:Phosphorus-Oxygen Lyases