title: Expression, purification and preliminary X-ray characterization of CTP synthetase from Thermus thermophilus HB8.
abstract: A recombinant form of the CTP synthetase from Thermus thermophilus HB8 (tCTPs) was grown as colourless crystals by the hanging-drop vapour-diffusion technique using ammonium sulfate or sodium citrate as a precipitating agent. The crystals belong to space group I222, with unit-cell parameters a = 88.2, b = 118.9, c = 142.7 A, alpha = beta = gamma = 90 degrees, and are most likely to contain a monomer in the asymmetric unit with a V(M) value of 3.07 A(3) Da(-1). The crystals obtained from ammonium sulfate and sodium citrate solutions diffract X-rays to a resolution of 2.25 A using synchrotron X-ray sources and to a resolution of 2.35 A using Cu Kalpha X-rays from a rotating-anode generator.
authors.affiliation: Department of Chemistry, Graduate School of Science, Osaka City University, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Goto
authors.forename: Rie
authors.initials: R
authors.lastname: Omi
authors.forename: Jun
authors.initials: J
authors.lastname: Hoseki
authors.forename: Noriko
authors.initials: N
authors.lastname: Nakagawa
authors.forename: Ikuko
authors.initials: I
authors.lastname: Miyahara
authors.forename: Ken
authors.initials: K
authors.lastname: Hirotsu
mesh_terms: D019731:Carbon-Nitrogen Ligases / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016963:Thermus thermophilus / Q000201:enzymology*
chemical_list: D011994:Recombinant Proteins; D019731:Carbon-Nitrogen Ligases; C019951:CTP synthetase