Peripheral nerve connections influence the appearance of neurosecretary material in neural sheath of ventral ganglion of the fly Sarcophaga bullata: an immunocytochemical study.
This study examined the role of the brain and peripheral connections with the target organs in the appearance of neurosecretary material within the dorsal neural sheath of the ventral ganglion of the fly S. bullata. Specifically, the accumulation of the neuropeptide FMRFamide (the neurosecretary material) was examined by immunocytochemistry. Immunoreactions were performed on: (1) a normal intact ventral ganglion, (2) an isolated ventral ganglion that was cultured in vivo, and (3) a ventral ganglion that was isolated by transection from the brain, but retained its peripheral nerve connections. The results demonstrate that (a) the neurons of the ganglia survive and exhibit FMRFamide immune reaction independent of their peripheral connections, and (b) the accumulation of neuropeptide in the dorsal neural sheath is controlled by intact peripheral nerve connections with the ganglion. It is suggested that in the absence of their peripheral connections, the axons of FMRFamide immunoreactive neurons fail to invade the neural sheath resulting in the accumulation of neurosecretary material.
Sivasubramanian
P
P
Department of Biology, University of New Brunswick, Fredericton, New Brunswick, Canada E3B 6E1. psivasub@unb.ca
Sood
Prem Prakash
PP
D000818:Animals; D004175:Diptera; D019835:FMRFamide / Q000096:biosynthesis*; D005724:Ganglia / Q000378:metabolism; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D009186:Myelin Sheath / Q000276:immunology / Q000378:metabolism*; D009924:Organ Culture Techniques; D017933:Peripheral Nervous System / Q000502:physiology*
D019835:FMRFamide