title: Comparison of ribotyping and repetitive extragenic palindromic-PCR for identification of fecal Escherichia coli from humans and animals.
abstract: This report compares the performances of two popular genotypic methods used for tracking the sources of fecal pollution in water, ribotyping and repetitive extragenic palindromic-PCR (rep-PCR). The rep-PCR was more accurate, reproducible, and efficient in associating DNA fingerprints of fecal Escherichia coli with human and animal hosts of origin.
authors.affiliation: Department of Veterinary Pathobiology, 201 Connaway Hall, University of Missouri, Columbia, MO 65211, USA. carsonc@missouri.edu
authors.forename: C Andrew
authors.initials: CA
authors.lastname: Carson
authors.forename: Brian L
authors.initials: BL
authors.lastname: Shear
authors.forename: Mark R
authors.initials: MR
authors.lastname: Ellersieck
authors.forename: Jennifer D
authors.initials: JD
authors.lastname: Schnell
mesh_terms: D000818:Animals; D002417:Cattle; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D004285:Dogs; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics; D005243:Feces / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D021521:Ribotyping
chemical_list: D004269:DNA, Bacterial