Engineering redox functions in a nucleic acid binding protein.
A nucleic acid binding protein, rop, has conserved topology with a number of redox proteins; this is exploited to engineer haem binding, expanding its function as a redox protein.
Wilson
Jon R
JR
Department of Biological Sciences, Biochemistry Building, Imperial College, London, UK SW7 2AY.
Caruana
Daren J
DJ
Gilardi
Gianfranco
G
D001426:Bacterial Proteins / Q000737:chemistry; D001665:Binding Sites; D004563:Electrochemistry / Q000379:methods; D006418:Heme / Q000737:chemistry; D010084:Oxidation-Reduction; D011487:Protein Conformation; D015202:Protein Engineering / Q000379:methods*; D016601:RNA-Binding Proteins / Q000737:chemistry*; D017186:Titrimetry
D001426:Bacterial Proteins; D016601:RNA-Binding Proteins; C054243:Rop protein, ColE1 plasmid; D006418:Heme