title: Mechanism of flavin mononucleotide cofactor binding to the Desulfovibrio vulgaris flavodoxin. 1. Kinetic evidence for cooperative effects associated with the binding of inorganic phosphate and the 5'-phosphate moiety of the cofactor.
abstract: The pathway(s) by which the flavin cofactor binds to the apoflavoprotein is the subject of some debate. The crystal and NMR structures of several different flavodoxins have provided some insight, although there is disagreement about the location of the initial interaction between the flavin mononucleotide (FMN) and the apoflavodoxin and the degree of protein conformational change associated with cofactor binding [Genzor, C. G., Perales-Alcon, A., Sancho, J., and Romero, A. (1996) Nat. Struct. Biol. 3, 329-332; Steensma, E., and van Mierlo, C. P. M. (1998) J. Mol. Biol. 282, 653-666]. Binding kinetics using stopped-flow spectrofluorimetry and phosphate competition studies were used to develop a model for flavin binding to the flavodoxin from Desulfovibrio vulgaris. In the presence of phosphate, the time course of fluorescence quenching associated with FMN binding to apoflavodoxin was biphasic, whereas riboflavin, which lacks the 5'-phosphate group of FMN, displayed monophasic binding kinetics. When the concentration of phosphate in solution was increased, the FMN binding rates of the two phases behaved differently; the rate of one phase decreased, while the rate of the other increased. A similar increase in the single phase associated with riboflavin binding was also observed. This has led to the following model. The binding of the flavin isoalloxazine ring to its subsite is dependent on the presence of a phosphate group in the phosphate-binding subsite. When phosphate is in the buffer solution, FMN can bind in either of two ways: by the initial insertion of the 5'-phosphate group followed by ring binding or, when inorganic phosphate from solution is bound, the insertion of the isoalloxazine ring first. Riboflavin, which lacks the phosphate moiety of FMN, binds only in the presence of inorganic phosphate, presumably due to the binding of this group in the phosphate-binding subsite. These results suggest that cooperative interactions exist between the phosphate subsite and the ring-binding region in the D. vulgaris flavodoxin that are necessary for isoalloxazine ring binding.
authors.affiliation: Department of Biochemistry and Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio 43210-1292, USA.
authors.forename: Tracey Arnold
authors.initials: TA
authors.lastname: Murray
authors.forename: Richard P
authors.initials: RP
authors.lastname: Swenson
mesh_terms: D000409:Alanine / Q000235:genetics; D001059:Apoproteins / Q000737:chemistry* / Q000235:genetics; D001216:Asparagine / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D016969:Desulfovibrio vulgaris / Q000737:chemistry* / Q000235:genetics; D005486:Flavin Mononucleotide / Q000737:chemistry*; D005418:Flavodoxin / Q000737:chemistry* / Q000235:genetics; D006639:Histidine / Q000235:genetics; D007700:Kinetics; D008956:Models, Chemical; D016297:Mutagenesis, Site-Directed; D010710:Phosphates / Q000737:chemistry*; D011485:Protein Binding / Q000235:genetics; D012256:Riboflavin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D013912:Threonine / Q000235:genetics; D014364:Tryptophan / Q000235:genetics; D014443:Tyrosine / Q000235:genetics
chemical_list: D001059:Apoproteins; D005418:Flavodoxin; D010710:Phosphates; C026932:apoflavodoxin; D013912:Threonine; D014443:Tyrosine; D006639:Histidine; D001216:Asparagine; D005486:Flavin Mononucleotide; D014364:Tryptophan; D000409:Alanine; D012256:Riboflavin