title: Prediction of measurement precision of apparatus using a chemometric tool in electrochemical detection of high-performance liquid chromatography.
abstract: The relative standard deviation (RSD) of measurements in high-performance liquid chromatography with electrochemical detection (HPLC-ECD) was predicted by a chemometric tool based on the 1/f fluctuation model which is made up of white noise and a Markov process, called the Function of Mutual Information (FUMI) theory. FUMI theory can provide aprecise and reliable detection limit from a single measurement of noise and signal in HPLC-ECD. To obtain RSD (n = 5) for determination of (-)-epicatechin at five concentrations required 12.5 h, while the predicted RSD by FUMI theory required only 0.5 h (one measurement). Moreover, to trace the source of instrumental noise, power spectra of chromatographic baseline were used. Selection of a suitable apparatus in HPLC-ECD system, acquisition of RSD, and detection limits for determination of catechins by HPLC-ECD were simply and easily made by this chemometric tool within a very short time. The use of the FUMI theory for the prediction of measuring precision was more efficient and the optimization was less time-consuming to be suited for determination.
authors.affiliation: School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kotani
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Rieko
authors.initials: R
authors.lastname: Matsuda
authors.forename: Fumiyo
authors.initials: F
authors.lastname: Kusu
mesh_terms: D002392:Catechin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D002392:Catechin