CD36-mediated endocytic uptake of advanced glycation end products (AGE) in mouse 3T3-L1 and human subcutaneous adipocytes.
Interaction of advanced glycation end products (AGE) with AGE receptors induces several cellular phenomena potentially relating to diabetic complications. We here show that AGE-modified bovine serum albumin (BSA) is endocytosed by adipocytes via CD36. Upon differentiation, 3T3-L1 and human subcutaneous adipose cells showed marked increases in endocytic uptake and subsequent degradation of [(125)I]AGE-BSA, which were inhibited effectively by the anti-CD36 antibody. Ligand specificity of CD36 for modified BSAs was compared with that of LOX-1 and scavenger receptor class A. Effect of fucoidan on [(125)I]AGE-BSA binding showed a sharp contrast to that on [(125)I]-oxidized low density lipoprotein. These results implicate that CD36-mediated interaction of AGE-modified proteins with adipocytes might play a pathological role in obesity or insulin-resistance.
Kuniyasu
Akihiko
A
Department of Biofunctional Chemistry, Faculty of Pharmaceutical Sciences, Kumamoto University, 5-1 Ohe-Honmachi, Japan.
Ohgami
Nobutaka
N
Hayashi
Shigeki
S
Miyazaki
Akira
A
Horiuchi
Seikoh
S
Nakayama
Hitoshi
H
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D016475:3T3 Cells; D017667:Adipocytes / Q000502:physiology*; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D015703:Antigens, CD / Q000276:immunology / Q000502:physiology*; D001692:Biological Transport; D018955:CD36 Antigens / Q000276:immunology / Q000502:physiology*; D003907:Dexamethasone / Q000494:pharmacology; D004705:Endocytosis / Q000502:physiology*; D017127:Glycation End Products, Advanced / Q000493:pharmacokinetics*; D006801:Humans; D051379:Mice; D012710:Serum Albumin, Bovine / Q000493:pharmacokinetics; D012867:Skin
D000906:Antibodies; D015703:Antigens, CD; D018955:CD36 Antigens; D017127:Glycation End Products, Advanced; C081628:advanced glycation end products-bovine serum albumin; D012710:Serum Albumin, Bovine; D003907:Dexamethasone; D015056:1-Methyl-3-isobutylxanthine