title: Slit and robo: expression patterns in lung development.
abstract: First described as an axonal guidance cue through its repulsive effect on neurons expressing its receptor Roundabout (Robo), the Slit ligand has effects on cell migration, axon branching and elongation. Indirect evidence implicates Slit and Robo in lung development. We now demonstrate that Slit-2 and Slit-3 are developmentally regulated in embryonic murine lung. Immunohistochemistry demonstrates Slit-2 and Slit-3 expression by the pulmonary mesenchyme and airway epithelium. Robo-1 and Robo-2 are also expressed by the developing mesenchyme and airway epithelium. As lung development progresses, Robo-1 and Robo-2 expression localizes to only the airway epithelium. We conclude Slit/Robo are expressed in temporo-spatially adjacent domains suggesting interactive roles in pulmonary bronchiolar development.
authors.affiliation: Pediatric Pulmonary Unit, Massachusetts General Hospital for Children, Harvard Medical School, Jackson 14, GRJ 1416, 55 Fruit St, Boston, MA 02114, USA.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Anselmo
authors.forename: Sussie
authors.initials: S
authors.lastname: Dalvin
authors.forename: Parthak
authors.initials: P
authors.lastname: Prodhan
authors.forename: Katsumi
authors.initials: K
authors.lastname: Komatsuzaki
authors.forename: Jeremy T
authors.initials: JT
authors.lastname: Aidlen
authors.forename: Jay J
authors.initials: JJ
authors.lastname: Schnitzer
authors.forename: Jane Y
authors.initials: JY
authors.lastname: Wu
authors.forename: T Bernard
authors.initials: TB
authors.lastname: Kinane
mesh_terms: D000818:Animals; D029721:Drosophila Proteins; D020869:Gene Expression Profiling; D008168:Lung / Q000196:embryology* / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics*; D000097905:Roundabout Proteins
chemical_list: D029721:Drosophila Proteins; D009419:Nerve Tissue Proteins; D011971:Receptors, Immunologic; D000097905:Roundabout Proteins; C066819:sli protein, Drosophila