A reagentless electrochemical biosensor based on a protein scaffold.
Apo-myoglobin, labeled with the environmentally sensitive redox probe RuII(NH3)4(1,10-phenanthroline-5-maleimide)2+, was immobilized onto gold electrodes modified with 11-mercaptoundecanoic acid and subsequently labeled with biotin; avidin binding to the immobilized biotin was specifically and quantitatively detected by a change in cyclic voltammetry of the co-immobilized probe.
Jhaveri
Sulay D
SD
Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375, USA.
Mauro
J Matthew
JM
Goldston
Harold M
HM
Schauer
Caroline L
CL
Tender
Leonard M
LM
Trammell
Scott A
SA
D000818:Animals; D001059:Apoproteins / Q000737:chemistry; D001360:Avidin / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D015336:Molecular Probe Techniques; D009211:Myoglobin / Q000737:chemistry*; D009942:Organometallic Compounds; D010084:Oxidation-Reduction; D015202:Protein Engineering; D012428:Ruthenium / Q000737:chemistry*; D014907:Whales
D001059:Apoproteins; D009211:Myoglobin; D009942:Organometallic Compounds; D001360:Avidin; D001710:Biotin; D012428:Ruthenium