Induction of apoptosis in an insect cell line, IPLB-Ld652Y, infected with nucleopolyhedroviruses.
Ld652Y cells derived from the gypsy moth, Lymantria dispar, were infected with seven different nucleopolyhedroviruses (NPVs) including those from Autographa californica, Bombyx mori (BmNPV), Hyphantria cunea (HycuNPV), Spodoptera exigua (SeMNPV), L. dispar, Orgyia pseudotsugata (OpMNPV) and Spodoptera litura (SpltMNPV). The results showed that Ld652Y cells infected with BmNPV, HycuNPV, SeMNPV, OpMNPV and SpltMNPV underwent apoptosis, displaying apoptotic bodies, characteristic DNA fragmentation and increased caspase-3-like protease activity; HycuNPV induced the most severe apoptosis. In HycuNPV-infected Ld652Y cells, a considerable amount of viral DNA was synthesized although there was no detectable yield of budded virions and polyhedrin. Northern blot and immunoblot analyses revealed that HycuNPV inhibitor of apoptosis 3 (IAP3), which has been shown to function in Sf9 cells, was expressed in HycuNPV-infected Ld652Y cells at a level higher than or comparable with that in HycuNPV-infected SpIm cells, which produced a high titre of progeny virions without any apoptotic response. These results imply that the relative ease of apoptosis induction in NPV-infected Ld652Y cells is largely dependent on inherent cellular properties rather than functions of the respective NPVs, and indicate that the defect in progeny virion production is not merely due to the virus-induced apoptosis in HycuNPV-infected Ld652Y cells.
Ishikawa
Hiroki
H
Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Ikeda
Motoko
M
Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Yanagimoto
Kenichi
K
Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Alves
Cristiano A Felipe
CAF
Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Katou
Yasuhiro
Y
Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Laviña-Caoili
Barbara A
BA
Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Kobayashi
Michihiro
M
Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
D000818:Animals; D017209:Apoptosis; D015152:Blotting, Northern; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D053938:DNA Fragmentation; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis; D015151:Immunoblotting; D002479:Inclusion Bodies; D051034:Inhibitor of Apoptosis Proteins; D007313:Insecta; D017924:Nucleopolyhedroviruses / Q000378:metabolism / Q000502:physiology*; D000077183:Occlusion Body Matrix Proteins; D012333:RNA, Messenger / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis; D015678:Viral Structural Proteins / Q000032:analysis / Q000096:biosynthesis; D014771:Virion / Q000302:isolation & purification; D014779:Virus Replication
D004279:DNA, Viral; D051034:Inhibitor of Apoptosis Proteins; D000077183:Occlusion Body Matrix Proteins; D012333:RNA, Messenger; D014764:Viral Proteins; D015678:Viral Structural Proteins; C086430:inhibitor of apoptosis, Nucleopolyhedrovirus; C009829:polyhedrin protein, Nucleopolyhedrovirus; D020169:Caspases