[Effect of transfection of hEndostatin gene on CNE2 cell xenograft growth in nude mice].
OBJECTIVE
Tumor angiogenesis play an important role in growth and metastasis of cancer. Angiogenesis inhibitors induce apoptosis in cancer by inhibiting tumor angiogenesis and have strong inhibiting effect on both growth and metastasis of cancer. This study was designed to explore the effect of transfection of human endostatin gene on nasopharyngeal carcinoma CNE2 cells xenograft growth in nude mice.


METHODS
The plasmids (pBlast-hIL-hEndostatin, pBlast-hEndostatin, and pBlast-MCS) were transfected and lipofectin-mediated into the CNE2 cell line. The biological activity of secreted hEndostain from gene-transferred cell lines was determined using MTT method in vitro. Then the transfected CNE2 cells were injected into the nude mice and tumorigenicity of CNE2 was observed in vivo.


RESULTS
The supernatant of CNE2 cell transfected with pBlast-hIL-hEndostatin effectively inhibited the growth of endothelial cell (ECV304). The volume and the weight of tumor in pBlast-hIL -hEndostatin transfecting cells group were less than those in control group (P< 0.01). The growth speed of tumor in pBlast-hIL-hEndostatin transfecting cells group was slower than that in control group.


CONCLUSIONS
Transfection of hEndostatin gene could inhibit CNE2 cell growth in nude mice.
Yue
Tao
T
Department of Biochemistry, Sun Yat-sen Medical College, Sun Yat-sen University, Guangzhou, Guangdong, 510080, PR China.
Zhang
Ping
P
Liu
Peng
P
Deng
Qing-Li
QL
Ji
Qiong-Mei
QM
Li
Xiu-Ying
XY
Zhu
Zhen-Yu
ZY
D000818:Animals; D015153:Blotting, Western; D015197:Carcinogenicity Tests; D002455:Cell Division; D003094:Collagen / Q000235:genetics / Q000627:therapeutic use*; D004195:Disease Models, Animal; D043169:Endostatins; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000473:pathology; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000517:prevention & control*; D010446:Peptide Fragments / Q000235:genetics / Q000627:therapeutic use*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
D043169:Endostatins; D010446:Peptide Fragments; D003094:Collagen