Effect of exposure to mitomycin C on cultured tympanic membrane fibroblasts.
OBJECTIVE
Recently, attempts have been made to prolong the patency of myringotomy site with topical use of mitomycin C (MMC). It has been shown that MMC inhibits mitosis and proliferation of ocular fibroblasts, however, there are no studies of MMC's effect on tympanic membrane fibroblasts. To investigate the effects of MMC on cultured human tympanic membrane fibroblasts and understand the cellular basis of MMC for maintain myringotomy patency, cultured fibroblasts were exposed to various concentrations of MMC for periods of 5-10 min.


METHODS
Effect of MMC on cultured fibroblasts was assessed by microscopic observation and cell viability test.


RESULTS
Dose-, time- dependent relationship of MMC on cultured fibroblasts was revealed. There was a significant difference between the inhibition effects of MMC at concentrations of 0.4 mg/ml and control following 5 and 10 min exposure intervals. Phase-contrast microscopy showed consistency with the antiproliferative effect of MMC at higher concentration.


CONCLUSIONS
Therefore, it would appear that intraoperative use of MMC could be effective in delaying the healing of the myringotomy site and extending the period of time for myringotomy patency.
Jang
Chul Ho
CH
Department of Otolaryngology, Wonkwang Medical School, Iksan, 570-711, South Korea. chul@wonkwang.ac.kr
Song
Chang-Hun
CH
Pak
Sok Cheon
SC
D016923:Cell Death / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000648:ultrastructure; D006801:Humans; D008858:Microscopy, Phase-Contrast; D016685:Mitomycin / Q000009:adverse effects* / Q000494:pharmacology*; D011336:Probability; D012016:Reference Values; D012680:Sensitivity and Specificity; D014432:Tympanic Membrane / Q000166:cytology* / Q000187:drug effects
D016685:Mitomycin