[Relationship between trophoblast cell's human leukocyte antigen G expression and early embryo development].
OBJECTIVE
To study the expression of soluble human leukocyte antigen G (sHLA-G) in trophoblast cells of spontaneous abortion after in vitro fertilization and embryo transfer (IVF-ET) and choriocarcinoma, and explore the relationship between sHLA-G expression in trophoblast cells and the early embryo development.


METHODS
14 cases of trophoblast cells of spontaneous abortion after IVF-ET and 6 cases of choriocarcinoma tissue has been detected, and which were compared with the 10 cases of trophoblast cells of normal villus.


RESULTS
(1) One case (1/14) has positive sHLA-G expression in IVF-ET group, and other cases in this group are negative or faint sHLA-G expression; ALL cases in choriocarcinoma group are strong positive sHLA-G expression; All cases in normal villous group are positive sHLA-G expression. (2) sHLA-G is expressed on the cytotrophoblast cells, especially on the extravillus cytotrophablast. The expression level of HLA-G is obviously decreased in the spontaneous abortion group compared with normal group. For single cells, the staining on the choriocarinoma cells are stronger than that of the normal villus cells.


CONCLUSIONS
High expression of sHLA-G in choriocarcinoma trophoblast cells and in trophoblast cells of normal villus and low expression in spontaneous abortion trophoblast cells indicating sHLA-G in trophoblast cells has some relationship with the early embryo development.
Wang
Qiong
Q
Department of Obstetric and Gynecology, First Affiliated Hospital of Sun Yat-Sen University of Medical Sciences, Guangzhou 510080, China.
Zhuang
Guanglun
G
Zhou
Canquan
C
Li
Jie
J
Zhang
Minfang
M
Xu
Yanwen
Y
Shu
Yimin
Y
D000022:Abortion, Spontaneous / Q000209:etiology; D000328:Adult; D004624:Embryo Transfer; D005314:Embryonic and Fetal Development; D005307:Fertilization in Vitro; D006680:HLA Antigens / Q000032:analysis*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D006801:Humans; D014327:Trophoblasts / Q000276:immunology*
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I