Purification and characterization of an alkaline lipase from a newly isolated Pseudomonas mendocina PK-12CS and chemoselective hydrolysis of fatty acid ester.
Lipase isolated from a soil isolate, Pseudomonas mendocina (PK-12CS) chemoselectively hydrolyzed the fatty ester group in presence of arbamate of compound 5-amino-2,4-dihydro-3H-1,2,4-triazole-3 ones, a class of compounds which are attractive starting materials for the synthesis of triazole annealed heterocycles. The enzymatic method provides an easy access to the synthesis of N-substituted glycine. Under optimized fermentation conditions the culture produced 3510 Lipolytic Units/mL of cell free fermentation broth in 20 h of fermentation. The purified lipase exhibited molecular mass of 80 kDa on SDS polyacrylamide gel electrophoresis. The enzyme was stable at room temperature for more than a month and expressed maximum activity at 37 degrees C and pH 8.
Jinwal
Umesh K
UK
Division of Fermentation Technology, Central Drug Research Institute, Lucknow-226 001, India.
Roy
Uma
U
Chowdhury
Abhijit R
AR
Bhaduri
A P
AP
Roy
P K
PK
D001426:Bacterial Proteins; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004952:Esters / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D006207:Half-Life; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008049:Lipase; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D011549:Pseudomonas / Q000201:enzymology*; D012997:Solvents; D013055:Spectrophotometry, Infrared; D013379:Substrate Specificity; D013696:Temperature; D014280:Triglycerides / Q000737:chemistry
D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D004952:Esters; D005227:Fatty Acids; D012997:Solvents; D014280:Triglycerides; D002265:Carboxylic Ester Hydrolases; C113518:LipA protein, Pseudomonas; D008049:Lipase