Expansion of human gammadelta T cells after in vitro stimulation with Campylobacter jejuni.
Campylobacter jejuni is currently the prime cause of food-borne bacterial gastro-enteritis. An important complication of C. jejuni enteritis is Guillain-Barré syndrome (GBS), an immune-mediated disorder of peripheral nerve tissue. Because little is known about T cell reactivity to C. jejuni, we have analyzed the in vitro immune response of normal individuals against five isolates of C. jejuni representing five different serotypes. We found a preferential expansion of peripheral blood gammadelta T cells after exposure to crude sonicates of all five C. jejuni serotypes. Expansion of gammadelta T cells was dependent on the presence of CD4+/alphabeta+ T cells in the cultures or addition of exogenous IL-2 or IL-15. C. jejuni stimulation was mediated via the TCR and appeared to be induced by a non-proteinaceous bacterial antigen, most likely of phosphoantigenic origin.
Van Rhijn
Ildiko
I
Departments of Neurology and Immunology, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands.
Van den Berg
Leonard H
LH
Ang
C Wim
CW
Admiraal
Joke
J
Logtenberg
T
T
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D016123:Campylobacter jejuni / Q000276:immunology*; D002455:Cell Division / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D019409:Interleukin-15 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D017422:Sequence Analysis, DNA; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology*
D000276:Adjuvants, Immunologic; D007135:Immunoglobulin Variable Region; D019409:Interleukin-15; D007376:Interleukin-2; D016692:Receptors, Antigen, T-Cell, gamma-delta