title: Adsorption of bovine serum albumin onto surface-modified polyhydroxyethyl methacrylate beads.
abstract: The adsorption of bovine serum albumin (BSA) onto poly(2-hydroxyethyl methacrylate) (PHEMA) beads modified by using the pair of hexamethylene diisocyonate-suberic acidbis-N-hydroxy succinimide has been studied as a function of protein concentration and adsorption time. The adsorption studies were carried out in phosphate buffer solution (PBS) at pH = 7.4. The isotherm data have been analysed using the Langmuir model and the adsorption parameters Q0 and b were calculated. It is determined that the adsorbed amount of BSA increases by the increase of the adsorption time and BSA concentration until a certain value. PHEMA beads were characterized by using FTIR spectra and SEM analysis. The adsorption of BSA onto PHEMA beads were clearly observed from SEM micrographs. The swelling tests of the beads were performed at 37 degrees C in PBS.
authors.affiliation: Gazi Universitesi, Fen Edebiyat Fakültesi, Kimya Bölümü, Teknikokullar, Ankara, Türkiye. mpulat@gazi.edu.tr
authors.forename: Mehlika
authors.initials: M
authors.lastname: Pulat
authors.forename: Elif
authors.initials: E
authors.lastname: Memiş
authors.forename: Menemşe
authors.initials: M
authors.lastname: Gümüşderelioğlu
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D008855:Microscopy, Electron, Scanning; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013816:Thermodynamics
chemical_list: D011102:Polyhydroxyethyl Methacrylate; D012710:Serum Albumin, Bovine