Determination of the antibacterial trovafloxacin by differential-pulse adsorptive stripping voltammetry.
A differential-pulse adsorptive stripping voltammetric method for the determination of trace amounts of the antibacterial trovafloxacin (TRFLX) is proposed. The optimal experimental parameters for the drug assay were: accumulation potential=-0.30 V (vs. Ag/AgCl), accumulation time=120 s, pulse amplitude=50 mV and scan rate=5 mV s(-1) in Britton-Robinson buffer (pH 4.5). The linear concentration range of application was 2.0-20.0 ng ml(-1) of TRFLX, with a relative standard deviation of 3.6% (for a level of 5.0 ng ml(-1)) and a detection limit of 0.6 ng ml(-1). The method was applied to determination of TRFLX in human urine and serum samples. It was validated using HPLC as a reference method. Recovery levels of the method reached 100% in all cases
Vílchez
José Luis
JL
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, E-18071 Granada, Spain.
Taoufiki
Jalila
J
Araujo
Lilia
L
Navalón
Alberto
A
D000328:Adult; D000890:Anti-Infective Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D024841:Fluoroquinolones; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008297:Male; D009287:Naphthyridines / Q000032:analysis* / Q000097:blood / Q000652:urine; D012015:Reference Standards; D015203:Reproducibility of Results; D012984:Software; D013056:Spectrophotometry, Ultraviolet
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D007202:Indicators and Reagents; D009287:Naphthyridines; C080163:trovafloxacin