title: Potentiality of a new compound for in vitro differentiation between halophilic and non-halophilic vibrios.
abstract: Sensitivity of 21 halophilic vibrios and 16 clinical isolates of non-halophilic vibrios was determined against a new possible antivibrio agent, a pyrimidine analogue, 4, 6-dimethylpyrimidine -2-thiol (4,6-DMPT). It appeared to be a vibriocidal agent, having a mean MIC and MBC of 32 microg/ml for halophilic strains and 64 microg/ml for non-halophilic strains and an LD50 of 300 mg/Kg body weight of mice. Thus, 4,6-DMPT may help an in vitro distinction between halophilic and non-halophilic vibrios. Sensitivity of these strains was also studied with respect to pteridine, crystal violet and Tween 80 hydrolysis as further markers distinguishing between these 2 groups which could also be differentiated by their growth on TCBS or/and CLED media.
authors.affiliation: Department of Medical Microbiology & Parasitology, University College of Medicine, Kolkata 700 020, India.
authors.forename: R
authors.initials: R
authors.lastname: Ray
authors.forename: A K
authors.initials: AK
authors.lastname: Das
authors.forename: N K
authors.initials: NK
authors.lastname: Dutta
authors.forename: A N
authors.initials: AN
authors.lastname: Chakrabarty
authors.forename: B N
authors.initials: BN
authors.lastname: Chaudhuri
authors.forename: S
authors.initials: S
authors.lastname: Seth
authors.forename: Sujata G
authors.initials: SG
authors.lastname: Dastidar
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D005840:Gentian Violet / Q000494:pharmacology; D006868:Hydrolysis; D051379:Mice; D008826:Microbial Sensitivity Tests; D011136:Polysorbates / Q000494:pharmacology; D011621:Pteridines / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D013501:Surface-Active Agents / Q000494:pharmacology; D014734:Vibrio cholerae / Q000145:classification* / Q000187:drug effects / Q000378:metabolism*; D014736:Vibrio parahaemolyticus / Q000145:classification* / Q000187:drug effects / Q000378:metabolism*
chemical_list: C104600:4,6-dimethylpyrimidine-2-thiol; D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D011136:Polysorbates; D011621:Pteridines; D011743:Pyrimidines; D013501:Surface-Active Agents; D005840:Gentian Violet