[Correlation between expression of Fas protein and hair cell apoptosis in basilar papilla of chicken].
OBJECTIVE
To study the relationship between expression of Fas protein and hair cell apoptosis following drug ototoxicity in the chicken inner ear.


METHODS
The damaged chicken inner ear model was developed by a subcutaneous injection of kanamycin at a daily dose of 100 mg/(kg.d) for 10 consecutive days. The Fas protein positive cells and apoptotic cells were examined by immunohistochemistry and TUNEL method at 1, 3, 7, 14, 21 days post-treatment.


RESULTS
TUNEL positive cells were localized to luminal layer of the sensory epithelia in the basilar papilla, there were much more apoptosis cells at the seventh day post-treatment. Fas positive cells began to increase at the first day post-treatment, and expression level of Fas protein increased to its peak at the third day after the last injection. At the fourteenth day post-treatment, TUNEL positive cells decreased following a low expression of Fas protein in the basilar papilla.


CONCLUSIONS
There is a relationship between hair cell apoptosis and high expression of Fas protein in chicken inner ear following chronic kanamycin ototoxicity, Fas protein may contribute to regulate and control hair cells apoptosis.
Lei
Li
L
Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
Wang
Jibao
J
Huang
Xiang
X
D000818:Animals; D017209:Apoptosis; D002645:Chickens; D005260:Female; D005839:Gentamicins / Q000633:toxicity; D006198:Hair Cells, Auditory / Q000378:metabolism* / Q000473:pathology; D008297:Male; D019014:fas Receptor / Q000096:biosynthesis*
D005839:Gentamicins; D019014:fas Receptor