[Alteration of multiple tumor metastatic genes with correlation to metastasis in ovarian carcinoma].
OBJECTIVE
To investigate the expression and mutation of MTA1, nm23H1 and E-cadherin(E-cad) genes in ovarian carcinoma (OC) in relation to lymph node (LN) metastasis.


METHODS
A panel of normal ovarian tissues, primary OC specimens and corresponding LNS was examined for mRNA expression and mutation of MTA1 and nm23H1 and protin expression of E-cad genes by using RT-PCR, RT-PCR-SSCP and immunohistochemistry.


RESULTS
The frequency of MTA1 over expression was 100%(7/7) in primary OC with metastasis but only 38.5%(5/13) in those without metastasis (P = 0.0103). Overexpression of MTA1 was observed in 87.5%(6/7) of LNS with metastasis but in only 23%(3/13) of LNS without metastasis (P = 0.0118). In contrast with MTA1, low expression of nm23H1 mRNA was seen in 7 of 7 OC with metastasis but only in 4 of 13(30%) of those without metastasis (P = 0.0043). Low nm23H1 expression was also seen in 7 of 7 LNS with metastasis but only in 5 of 13 (38.5%) nonmetastatic LNS (P = 0.0102). Meantime, no expression of E-cad protein was observed in 7 of 7 OC with metastasis but in 6 of 13(46.2%) of those without metastasis (P = 0.044). In correlation analysis of the three genes, MTA1 reversely correlated with nm23H1 and E-cad respectively (r = -0.903, -0.803), and positive correlation existed between nm23H1 and E-cad (r = 0.724). No mutation of MTA1, nm23H1 and was found by SSCP analysis.


CONCLUSIONS
The mRNA expression of MTA1, nm23H1 and E-cad is positively and negatively correlated with LN metastasis. The expression abnormalities but not the mutations of the three genes are frequent events related to LN metastasis of ovarian cancer.
Huang
Guangqi
G
Institute of Cancer Research, West China Hospital, Sichuan University, Chengdu 610041, China.
He
Guoli
G
Song
Yi
Y
D015820:Cadherins / Q000096:biosynthesis / Q000235:genetics*; D018284:Cystadenocarcinoma, Serous / Q000235:genetics / Q000556:secondary; D005260:Female; D006655:Histone Deacetylases; D006801:Humans; D008207:Lymphatic Metastasis; D020559:Monomeric GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D009154:Mutation; D054778:NM23 Nucleoside Diphosphate Kinases; D009361:Neoplasm Invasiveness; D009701:Nucleoside-Diphosphate Kinase; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*
D015820:Cadherins; C089320:MTA1 protein, human; D054778:NM23 Nucleoside Diphosphate Kinases; D011506:Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D009701:Nucleoside-Diphosphate Kinase; D006655:Histone Deacetylases; D020559:Monomeric GTP-Binding Proteins