[Short time bacterial endotoxins test for positron emission tomography by means of positively charged filters].
Positron emission tomography (PET) radiotracers have very short physical half-lives. It is hard to complete a bacterial endotoxins test prior to release from medical institutes. For endotoxin quantitative determination, limulus amebocyte lysate (LAL) reagent and kinetic-turbidimetry system were previously developed. We investigated the possibility of a short time test by means of positively charged filters. As a result of this study, the effects of positively charged filters on endotoin removal were over 99.5% for [18F]FDG and [18F]NaF, which were contaminated with the indicated concentration of endotoxin. Combining this filter and the kinetic-turbidimetric method, it was possible to complete a bacterial endotoxins test in 5 min prior to the patient's administration. This test should be required prior to release for PET radiopharmaceutical quality control. It has been suggested that this combination is a good method for this purpose.
Nakazawa
Nobuhiro
N
Nishijin Hospital.
Wakita
Kazuo
K
Mineura
Katsuyoshi
K
Nakamura
Masaru
M
Fujii
Ryou
R
Nakanishi
Hirotomo
H
Uji
Yoko
Y
Matsuura
Shirou
S
Itani
Kenji
K
Kanatsuna
Takahiro
T
Ido
Tatsuo
T
Imahori
Yoshio
Y
D004340:Drug Contamination / Q000517:prevention & control*; D004731:Endotoxins / Q000032:analysis / Q000302:isolation & purification*; D019788:Fluorodeoxyglucose F18; D006863:Hydrogen-Ion Concentration; D008851:Micropore Filters; D009391:Nephelometry and Turbidimetry; D011786:Quality Control; D019275:Radiopharmaceuticals; D012969:Sodium Fluoride; D014055:Tomography, Emission-Computed
D004731:Endotoxins; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; D012969:Sodium Fluoride