Effect of pH and sodium chloride on the strength and selectivity of the interaction of gamma [correction for tau] -cyclodextrin with some antisense nucleosides.
The influence of pH and the concentration of sodium chloride on the strength and selectivity of the interaction of twelve 8-substituted-2'-deoxyadenosine and sixteen 5-substituted-2'-deoxyuridine derivatives with gamma-cyclodextrin (GCD) have been studied by the spectral mapping technique (SPM). The potency values of the spectral map were regarded as indicators of the capability of antisense nucleosides and GCD to interact simultaneously taking into consideration all relevant data. It has been established that the strength of interaction is highest in acetic and lowest in alkaline solutions, and the selectivities of acidic, alkaline and salt solutions are markedly different. The length of hydrophobic alkyl substituents in antisense molecules influenced both the strength and selectivity of the interaction. The character of the base structure affected only the selectivity.
Cserháti
Tibor
T
Chemical Research Center, Institute of Chemistry, Hungarian Academy of Sciences, PO Box 17, 1525 Budapest, Hungary. forgacs@cric.chemres.hu
Forgács
Esther
E
D016375:Antisense Elements (Genetics) / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry*; D003839:Deoxyadenosines / Q000737:chemistry*; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D012965:Sodium Chloride / Q000737:chemistry*; D013329:Structure-Activity Relationship; D047408:gamma-Cyclodextrins
D016375:Antisense Elements (Genetics); D003505:Cyclodextrins; D003839:Deoxyadenosines; D047408:gamma-Cyclodextrins; D012965:Sodium Chloride; C023792:gamma-cyclodextrin; D003857:Deoxyuridine