[Drug sensitivity and beta-lactamase producibility of various types of bacteria clinically isolated during the period from December 1999 to February 2000].
beta-Lactamase activity and drug sensitivity were measured in 744 strains from 8 species of bacteria isolated at medical institutions in Chikugo District of Fukuoka Prefecture during the period from December 1999 to February 2000. Nitrocefin test revealed that beta-lactamase was positive in 48% of S. aureus, 7% of H. influenzae, and 92% of M. catarrhalis, and acidometry revealed that penicillinase/cephalosporinase were positive in 13%/14% of E. coli, 22%/8% of K. pneumoniae, 47%/97% of E. cloacae, 3%/65% of S. marcescens, and 10%/36% of P. aeruginosa. Based on the assessment of the MIC values of various types of antibacterial drugs for beta-lactamase-producing strains, there were 11 strains (1 strain of K. pneumonia, 6 strains of E. cloacae, and 4 strains of P. aeruginosa) of class-B beta-lactamase-producing bacteria out of a total of 496 strains of Enterobacteriaceae and Pseudomonas aeruginosa. The results of PCR analysis suggested that 1 strain of K. pneumonia, 1 strain of E. cloacae, and 4 strains of P. aeruginosa produced metallo-beta-lactamase. There was no strain (E. coli and K. pneumoniae) of ESBL-producing bacteria. BLNAR strains, on the other hand, were found in 9% (9/100) of H. influenzae.
Hashimoto
Kouji
K
Department of Laboratory Medicine, Kurume University Hospital.
Kinosita
Chie
C
Yoshinaga
Hideko
H
Sonda
Yukari
Y
Itoyama
Takako
T
Oyamada
Kazunori
K
Kajimura
Katsunari
K
Kondou
Masaharu
M
Sagawa
Kimitaka
K
Ouno
Tadashi
T
Kawamoto
Keiko
K
Souda
Katsumi
K
Hara
Kazumi
K
Kakihara
Miyako
M
Tanaka
Tetsuo
T
Ryutou
Miwako
M
Shichiji
Akiko
A
Nakashima
Kayoko
K
Ishibashi
Kazushige
K
Tabira
Reiko
R
Yamamoto
Shigeko
S
Nishida
Masahiro
M
Tsutsumi
Yasunori
Y
D001419:Bacteria / Q000187:drug effects* / Q000201:enzymology*; D024881:Drug Resistance, Bacterial; D016972:Enterobacter cloacae / Q000187:drug effects / Q000201:enzymology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D006193:Haemophilus influenzae / Q000187:drug effects / Q000201:enzymology; D007711:Klebsiella pneumoniae / Q000187:drug effects; D001936:Moraxella catarrhalis / Q000187:drug effects / Q000201:enzymology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology; D012706:Serratia marcescens / Q000187:drug effects / Q000201:enzymology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology; D001618:beta-Lactamases / Q000096:biosynthesis*
D001618:beta-Lactamases