Inhibitory effects of triterpenes isolated from Hoelen on free radical-induced lysis of red blood cells.
Hoelen, sclederma of Poria cocos Wolf, has long been used as a sedative and diuretic in traditional medicine. Formerly, we demonstrated that Hoelen in vitro protects red blood cells from AAPH-induced hemolysis. In this study, tests were carried out to identify the main ingredient of Hoelen that has the scavenging effect on free-radicals. Triterpene carboxylic acids isolated from the methanol extract of Hoelen, i.e. pachymic acid, polyporenic acid, 3-epidehydrotumulosic acid, 3beta-hydroxylanosta-7,9(11), 24-trien-21-oic acid and 3-o-acetyl-16 alpha -hydroxytrametenolic acid, were found to have inhibitory activities against AAPH-induced lysis of red blood cells.
Sekiya
Nobuyasu
N
Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan.
Goto
Hirozo
H
Shimada
Yutaka
Y
Endo
Yuichi
Y
Sakakibara
Iwao
I
Terasawa
Katsutoshi
K
D000578:Amidines; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects*; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D020072:Polyporales; D012016:Reference Values; D014315:Triterpenes / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use
D000578:Amidines; D016166:Free Radical Scavengers; D010936:Plant Extracts; D014315:Triterpenes; C046728:2,2'-azobis(2-amidinopropane); C102487:pachymic acid