Separation of proteins on polymeric stationary phases grafted with various amine groups.
Six polymeric stationary phases with various amine groups were prepared by surface grafting of glycidyl methacrylate on silica gel surface and its subsequent amination. The six kinds of amines, namely, triethylamine, diethylamine, ethylenediamine (EDA), hexaethylenediamine (HEDA), diethylenetriamine (DETA) and triethylenetetraamine (TETA) were used in this study. The separation of bovine serum albumin (BSA) and chicken egg albumin (CEA) on the polymeric stationary phase with various amines was investigated. The affinity degree of BSA was higher than CEA for the EDA, HEDA and DETA columns, whereas the affinity degree of CEA was higher than BSA for the TETA column.
Choi
Seong-Ho
SH
Department of Chemistry, Graduate School, Kyungpook National University, Daegu 702-701, South Korea.
Hwang
Young-Mi
YM
Lee
Kwang-Pill
KP
D000588:Amines / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D011506:Proteins / Q000302:isolation & purification*; D017550:Spectroscopy, Fourier Transform Infrared
D000588:Amines; D011506:Proteins