Analysis of aqueous 2,4,6-trinitrotoluene (TNT) using a fluorescent displacement immunoassay.
We report a rapid, simple, and sensitive assay that is potentially amenable to high throughput screening for analysis of 2,4,6-trinitrotoluene (TNT) present in aqueous solutions. The assay is based on the change in fluorescence emission intensity of a fluorescently labeled TNT analogue pre-bound to an anti-TNT antibody that occurs upon its competitive displacement by TNT. The assay can be performed in both cuvette- and 96-well plate-based formats. TNT at a level of 0.5 micro g L(-1) (0.5 ppb) was detected in phosphate buffered saline; detection improved to 0.05 micro g L(-1) (0.05 ppb) for TNT dissolved in artificial seawater.
Goldman
Ellen R
ER
US Naval Research Laboratory, Center for Bio/Molecular Science and Engineering, DC 20375, Washington, USA. erg@cbmse.nrl.navy.mil
Anderson
George P
GP
Lebedev
Nikolai
N
Lingerfelt
Brian M
BM
Winter
Phan T
PT
Patterson
Charles H
CH
Mauro
J Matthew
JM
D000906:Antibodies; D002232:Carbocyanines; D004784:Environmental Monitoring / Q000379:methods*; D005456:Fluorescent Dyes; D007118:Immunoassay; D012623:Seawater / Q000032:analysis; D017186:Titrimetry; D014303:Trinitrotoluene / Q000032:analysis* / Q000276:immunology; D014873:Water Pollutants / Q000032:analysis*
D000906:Antibodies; D002232:Carbocyanines; D005456:Fluorescent Dyes; D014873:Water Pollutants; D014303:Trinitrotoluene