Seasonal variation in plasma catecholamines and adipose tissue lipolysis in adult female green sea turtles (Chelonia mydas).
We investigated three aspects of potential interrenal regulation of reproduction in female green sea turtles, Chelonia mydas. First, seasonal trends in plasma catecholamines were examined from female C. mydas at different stages of their reproductive cycles. Second, variation in catecholamine levels during a nesting season were analysed in relation to restraint time, and ecological variables such as nesting habitat, body size, and reproductive investment. Third, catecholamine and corticosterone (CORT) induced lipolysis was investigated with adipose tissue collected from gravid green turtles, using in vitro incubations. Plasma epinephrine (EPI) was lowest in non-vitellogenic (1.55 +/- 0.26 ng/ml) and post-breeding (1.57 +/- 0.22 ng/ml) females, and highest in courting females (2.87 +/- 0.28). Concentrations of norepinephrine (NE) and EPI were relatively constant throughout a nesting season, and not significantly related to restraint time, reproductive investment or nesting habitat. In vitro concentrations of CORT (>3 ng/ml) and NE (2 ng/ml) induced significant release of glycerol after 6h of incubation. Epinephrine tended to induce an antilipolytic affect at low concentrations (0.25 ng/ml) and a net lipolytic response at higher concentrations (>1 ng/ml). Our data suggest that EPI may play a role in regulating body condition during vitellogenesis, and maintaining energy stores during prolonged aphagia during courtship and nesting in female green sea turtles. Furthermore, we provide preliminary evidence that suggests that catecholamine production may be either down regulated or de-sensitised in gravid female C. mydas.
Hamann
Mark
M
Department of Anatomy and Developmental Biology, School of Biomedical Sciences, The University of Queensland, St Lucia, QLD 4072, Australia. mark.hamann@iucn.org.vn
Limpus
Colin J
CJ
Whittier
Joan M
JM
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D003345:Corticosterone / Q000378:metabolism; D003380:Courtship; D004734:Energy Metabolism / Q000502:physiology; D004837:Epinephrine / Q000097:blood*; D005260:Female; D005990:Glycerol / Q000378:metabolism; D066298:In Vitro Techniques; D008066:Lipolysis / Q000502:physiology*; D009425:Nesting Behavior / Q000502:physiology*; D009638:Norepinephrine / Q000097:blood*; D012098:Reproduction / Q000502:physiology; D012621:Seasons; D013312:Stress, Physiological / Q000378:metabolism; D014426:Turtles / Q000378:metabolism*; D014818:Vitellogenesis / Q000502:physiology
D005990:Glycerol; D003345:Corticosterone; D009638:Norepinephrine; D004837:Epinephrine