title: Species-specific primer for identification of Anopheles quadriannulatus sp. B (Diptera: Culicidae) from Ethiopia using a multiplex polymerase chain reaction assay.
abstract: Anopheles quadriannulatus Theobald historically has been reported from southern Africa, Zanzibar islands, and Ethiopia. However, based on evidences of genetic incompatibility between crosses of South African and Ethiopian populations, the population from Ethiopia was recently reported as a distinct species designated as An. quadriannulatus sp. B. An. quadriannulatus sp. A, denoted the southern African population. To distinguish the two populations, the IGS (intergenic spacer) region of rDNA was sequenced to design a primer specific for An. quadriannulatus sp. B. A cocktail polymerase chain reaction (PCR) involving Anopheles gambiae Giles universal (UN) primer, the new primer and other primers specific for members of the An. gambiae complex produced the expected diagnostic products for the respective species. Using extracted DNA and crushed body parts as sources of template DNA, this assay was reliably used to identify samples of An. quadriannulatus sp. B.
authors.affiliation: Medical Entomology, Department of Clinical Microbiology and Infectious Diseases, School of Pathology of the National Health Laboratory Services and the University of Witwatersrand, PO Box 1038, Johannesburg 2000, South Africa. messayg@hotmail.com
authors.forename: Messay
authors.initials: M
authors.lastname: Fettene
authors.forename: Emmanuel A
authors.initials: EA
authors.lastname: Temu
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D005002:Ethiopia; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013019:South Africa; D013045:Species Specificity
chemical_list: D017931:DNA Primers