Mechanistic studies of the effect of hydroxypropyl-beta-cyclodextrin on in vitro transdermal permeation of corticosterone through hairless mouse skin.
Literature reports reveal that the issue of whether cyclodextrins may act as skin permeation enhancers has not been resolved. Accordingly, in vitro skin transport studies were conducted to address this question. Corticosterone (3H-CS and/or non-radiolabeled CS) was chosen as the model permeant for transport experiments with hairless mouse skin (HMS) and with a synthetic cellulose membrane of 500 molecular weight cut off (MWCO), the latter to help establish baseline behavior. Hydroxypropyl-beta-cyclodextrin (HPbetaCD) was selected as the representative cyclodextrin. The CS/HPbetaCD complexation constant was determined both from solubility data (saturation conditions) in phosphate buffered saline (PBS), pH 7.4 and with data obtained from PBS/silicone polymer partitioning experiments, the latter experiments permitting the determination of the complexation constant at low CS concentrations. These results were used in the calculations of the free CS concentrations in the donor chamber of the transport experiments. The CS transport experiments were conducted at CS solubility saturation and under supersaturation (resulting from autoclaving at 121 degrees C) conditions as well at very low (tracer level) concentrations. The effect of polyvinylpyrrolidone as a solution additive was also evaluated. The following were the key outcomes of this study. Contrary to literature reports, there was no evidence that HPbetaCD is an enhancer for CS transport through HMS. The CS permeability coefficient values obtained with HMS in all of the experiments were found to be the same within experimental error when calculated on the basis of the free CS concentration as the driving force for permeation. The constancy of the permeability coefficient in the presence and absence of HPbetaCD is interpreted to mean that, in these experiments, HPbetaCD did not alter the barrier properties of HMS stratum corneum to any significant extent nor did it enhance CS transport in any other manner such as by a carrier mechanism involving the aqueous boundary layer or by a carrier mechanism within the stratum corneum.
Shaker
D S
DS
Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, 30 South 2000 East, 213 Skaggs Hall, Salt Lake City, UT 84112, USA. dalsamuel@hotmail.com
Ghanem
A-H
AH
Li
S K
SK
Warner
K S
KS
Hashem
F M
FM
Higuchi
W I
WI
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000277:Adjuvants, Pharmaceutic / Q000737:chemistry / Q000494:pharmacology*; D000279:Administration, Cutaneous; D000818:Animals; D001692:Biological Transport; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D003345:Corticosterone / Q000737:chemistry / Q000493:pharmacokinetics*; D003505:Cyclodextrins / Q000737:chemistry / Q000494:pharmacology*; D004355:Drug Stability; D005260:Female; D006358:Hot Temperature; D066298:In Vitro Techniques; D008567:Membranes, Artificial; D051379:Mice; D008812:Mice, Hairless; D010539:Permeability; D011205:Povidone / Q000737:chemistry / Q000494:pharmacology; D012869:Skin Absorption / Q000187:drug effects; D012995:Solubility; D047392:beta-Cyclodextrins
D000277:Adjuvants, Pharmaceutic; D003505:Cyclodextrins; D008567:Membranes, Artificial; D047392:beta-Cyclodextrins; C020253:cellulose ester plastic; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D002482:Cellulose; D011205:Povidone; D003345:Corticosterone