An amperometric immunosensor based on an electrochemically pretreated carbon-paraffin electrode for complement III (C3) assay.
An electrochemical immunosensor based on the adsorption of anti-complement III antibody onto an electrochemical pretreated carbon-paraffin electrode has been proposed for the detection of complement III (C(3)). The competitive immunoassay format was adopted with horseradish peroxide-C(3) (HRP-C(3)) as a tracer, 3,3'5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide as the enzyme substrates. In order to measure the amount of HRP-C(3) binding onto the electrode surface, the product of the enzyme catalytic reaction was detected at 100 mV (vs. Ag/AgCl reference electrode). The system was optimized to realize a reliable determination of C(3) in the range of 0.06-10 microg/ml. It exhibits some advantages, such as simplicity of fabrication, rapidity of measurement, and satisfactory sensitivity and reproducibility.
Zhou
Ya-Min
YM
State Key Laboratory for Chemo/Biosensing Technology and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Hu
Shen-Qin
SQ
Shen
Gou-Li
GL
Yu
Ru-Qin
RQ
D000906:Antibodies / Q000032:analysis / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002244:Carbon; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D003176:Complement C3 / Q000032:analysis* / Q000276:immunology; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006735:Horseradish Peroxidase / Q000276:immunology; D007124:Immunoenzyme Techniques / Q000295:instrumentation / Q000379:methods*; D010232:Paraffin; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000906:Antibodies; D020099:Coated Materials, Biocompatible; D003176:Complement C3; D002244:Carbon; D010232:Paraffin; D006735:Horseradish Peroxidase