A new method for the preparation of unprotected peptides on biocompatible resins with application in combinatorial chemistry.
[formula: see text] A synthetic strategy for the preparation of side chain free peptides on biocompatible solid supports is described. Final peptide detachment is afforded in mild basic conditions with no presence of scavengers or other additives, thus allowing single peptide-resin beads to be cleaved in mass spectrometry sample plates for direct sequencing using MALDI-TOF post-source decay. This methodology offers clear advantages for the development of one-bead--one-compound combinatorial libraries in addition to parallel and regular synthesis of peptides.
Pastor
Jose J
JJ
Institut de Recerca Biomèdica de Barcelona, Parc Científic de Barcelona, Universitat de Barcelona, 08028 Barcelona, Spain.
Fernández
Irene
I
Rabanal
Francesc
F
Giralt
Ernest
E
D000595:Amino Acid Sequence; D001672:Biocompatible Materials / Q000737:chemistry; D020650:Combinatorial Chemistry Techniques; D019151:Peptide Library; D010455:Peptides / Q000138:chemical synthesis*; D012117:Resins, Synthetic / Q000737:chemistry; D020539:Sequence Analysis, Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001672:Biocompatible Materials; D019151:Peptide Library; D010455:Peptides; D012117:Resins, Synthetic