An inexpensive sample mold for pulsed-field electrophoresis.
Specimens for pulsed-field gel electrophoresis (PFGE) are formed using a plug mold. We report a technique which uses a disposable polyethylene pipette to prepare our specimen plug. We also report a convenient technique to handle portions of the plug used for the typical PFGE manipulations.
Sheneman
Damon E
DE
Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ 85308, USA.
Katz
D Sue
DS
D003362:Cost-Benefit Analysis; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000191:economics / Q000295:instrumentation*; D010969:Plastics
D004269:DNA, Bacterial; D010969:Plastics