title: Effect of female sex steroids on human endometrial CD16neg CD56bright natural killer cells.
abstract: OBJECTIVE
  To clarify whether female sex steroids directly affect the bioactivity of the human endometrial CD16neg CD56bright natural killer (NK) cells.
  
  
  METHODS
  In vitro study.
  
  
  METHODS
  University obstetrics and gynecology department.
  
  
  METHODS
  Thirteen women with histologically normal endometrium who were undergoing hysterectomy and seven women during the first trimester of pregnancy who were undergoing selective termination.
  
  
  METHODS
  Endometrium or decidua was obtained.
  
  
  METHODS
  The effects of 17beta-estradiol or progesterone (10(-6), 10(-7), and 10(-8) M) on the proliferation, cytolytic activity, and cytokine secretion of the isolated endometrial CD16neg CD56bright NK cells were examined using a 3H-thymidine incorporation assay, 51Cr-releasing assay, and enzyme-linked immunosorbent assay, respectively.
  
  
  RESULTS
  Neither 17beta-estradiol nor progesterone had significant effects on the proliferation, cytolytic activity, and cytokine secretion of endometrial CD16neg CD56bright NK cells.
  
  
  CONCLUSIONS
  Female sex steroids do not directly affect the bioactivity of the human endometrial CD16neg CD56bright NK cells.
authors.affiliation: Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto, Japan. kitaya@koto.kpu-m.ac.jp
authors.forename: Kotaro
authors.initials: K
authors.lastname: Kitaya
authors.forename: Jinsuke
authors.initials: J
authors.lastname: Yasuda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nakayama
authors.forename: Shinji
authors.initials: S
authors.lastname: Fushiki
authors.forename: Hideo
authors.initials: H
authors.lastname: Honjo
mesh_terms: D000328:Adult; D019002:CD56 Antigen / Q000096:biosynthesis* / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000502:physiology; D003656:Decidua / Q000276:immunology / Q000378:metabolism; D004717:Endometrium / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D004958:Estradiol / Q000276:immunology / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D011247:Pregnancy; D011374:Progesterone / Q000276:immunology / Q000494:pharmacology*; D017452:Receptors, IgG / Q000096:biosynthesis* / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D019002:CD56 Antigen; D017452:Receptors, IgG; D014409:Tumor Necrosis Factor-alpha; D011374:Progesterone; D004958:Estradiol; D007371:Interferon-gamma; D013936:Thymidine