[Effects of epidermal growth factors on the proliferation and metabolism of A/J mouse embryonic palatal cells].
OBJECTIVE
The purpose of this study was to investigate the effects of epidermal growth factors (EGFs) with different concentration on the OD, DNA, protein, and PGE2 of A/J mouse embryonic palatal shelves cells (A/J MEPC) isolated from embryonic palatal shelves.


METHODS
The mouse embryonic palatal shelves cells were grown in different 39 pores (or bottles) with 9 gradient concentrations of EGF (0.005, 0.010, 0.050, 0.100, 0.500, 1.000, 5.000, 10.000, 50.000 ng/ml), and four pores were prepared for the same concentration, then the OD, DNA, protein and PGE2 of A/J MEPC were measured after 1 day, 3 days and 5 days.


RESULTS
EGFs stimulated DNA and PGE2 synthesis of A/J MEPC, and augmented proliferation index (PIX). Their effects were very obvious in promoting the proliferation of A/J MEPC, when the concentration was 10.000 ng/ml.


CONCLUSIONS
EGF may be important in regulating proliferation and metabolism of embryonic palatal shelves cells.
Jinhu
Sun
S
College of Stomatology, Guangxi Medical University.
Shi
Bing
B
Wang
Dazhang
D
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D015232:Dinoprostone / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D051379:Mice; D010159:Palate / Q000166:cytology*
D004815:Epidermal Growth Factor; D004247:DNA; D015232:Dinoprostone