title: Separation of twenty underivatized essential amino acids by capillary zone electrophoresis with contactless conductivity detection.
abstract: Twenty underivatized essential amino acids were separated using capillary zone electrophoresis and consequently detected with contactless conductivity detection (CCD). A simple acidic background electrolyte (BGE) containing 2.3 M acetic acid and 0.1% w/w hydroxyethylcellulose (HEC) allowed the electrophoretic separation and sensitive detection of all 20 essential amino acids in their underivatized cationic form. The addition of HEC to the BGE suppressed both, electroosmotic flow and analyte adsorption on the capillary surface resulting in an excellent migration time reproducibility and a very good analyte peak symmetry. Additionally, the HEC addition significantly reduced the noise and long-term fluctuations of the CCD baseline. The optimized electrophoretic separation method together with the CCD was proved to be a powerful technique for determination of amino acid profiles in various natural samples, like beer, yeast, urine, saliva, and herb extracts.
authors.affiliation: Department of Analytical Chemistry, Faclty of Science, Charles Unviersity, Pragye, Czech Republic. pcoufal@natur.cuni.cz
authors.forename: Pavel
authors.initials: P
authors.lastname: Coufal
authors.forename: Jirí
authors.initials: J
authors.lastname: Zuska
authors.forename: Tom
authors.initials: T
authors.lastname: van de Goor
authors.forename: Vonda
authors.initials: V
authors.lastname: Smith
authors.forename: Bohuslav
authors.initials: B
authors.lastname: Gas
mesh_terms: D000601:Amino Acids, Essential / Q000302:isolation & purification*; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods*
chemical_list: D000601:Amino Acids, Essential