title: High-throughput protein sequencing.
abstract: We have designed and implemented an autosampler that provides additional sample capacity on a commercial protein sequencer. The autosampler attaches to a standard ABI Procise sequencer, enabling a single-sample cartridge to hold up to six separate samples. The autosampler is used in combination with faster Edman cycles and a rapid 12-min PTH separation to significantly increase the speed of automated protein sequencing. We also describe Edman chemistry modifications that improve coupling efficiency and prevent internal cleavage that can occur when samples are sequenced in the standard glass cartridges.
authors.affiliation: Department of Protein Chemistry, Genentech, Inc., 1 DNA Way, South San Francisco, California 94080, USA.
authors.forename: Victoria
authors.initials: V
authors.lastname: Pham
authors.forename: Jake
authors.initials: J
authors.lastname: Tropea
authors.forename: Suzy
authors.initials: S
authors.lastname: Wong
authors.forename: James
authors.initials: J
authors.lastname: Quach
authors.forename: William J
authors.initials: WJ
authors.lastname: Henzel
mesh_terms: D000818:Animals; D001331:Automation; D004867:Equipment Design; D004864:Equipment and Supplies; D006801:Humans; D007202:Indicators and Reagents; D020539:Sequence Analysis, Protein / Q000295:instrumentation* / Q000379:methods
chemical_list: D007202:Indicators and Reagents