[Study on DNA oxidative damage of O3 aging model in mice].
OBJECTIVE
This study was aimed to detect the animals' DNA oxidative damage.


METHODS
A free radical mouse aging model was established by putting the mouse in a device for inhaling quantities of ozone (O3). Single cell gel electrophoresis (SCGE) was used for assessing the splenolymphocytes' DNA damage.


RESULTS
The appearance rate of comet and the length grade of DNA migration for the young control group (P < 0.05). No significant difference in the degree of DNA damage was observed between the O3 model group and the natural aging group(P > 0.05).


CONCLUSIONS
The DNA damage of O3 animal model is consistent with that of natural aging animal; this animal model DNA damage of O3 animal model is consistent with that of natural aging animal; this animal model and the method of SCGE are useful for future researches on aging and antiaging medicine.
Xing
H
H
Department of Physics, School of Basic Medical Sciences, WCUMS, Chengdu 610041, China.
Hu
X
X
Liu
H
H
Li
Y
Y
Chen
Y
Y
D000375:Aging / Q000235:genetics*; D000818:Animals; D020552:Comet Assay; D004249:DNA Damage; D004195:Disease Models, Animal; D005609:Free Radicals; D051379:Mice; D010126:Ozone
D005609:Free Radicals; D010126:Ozone