title: Detection of cytoplasmic CD antigens within normal human peripheral blood leucocytes.
abstract: Polymorphonuclear neutrophils (PMNs) are capable of synthesizing various pro-inflammatory cytokines which may indirectly influence specific immune responses. PMNs may also have the capacity to present foreign peptides to helper T cells (Th cells). In support of this hypothesis, recent studies have shown that neutrophils, when activated by the correct combination of cytokines, can be induced to express cell surface major histocompatibility complex (MHC) Class II (DR) antigen, CD80 (B7.1) and CD86 (B7.2): molecules required for antigen presentation and subsequent T-cell activation. In this study we have used normal "resting" human peripheral blood neutrophils and demonstrated, using a mild fixation and permeabilization protocol, significant cytoplasmic "stores" of these molecules known to be important in antigen presentation. Cytoplasmic MHC Class II antigen was found with two out of 20 normal donors tested whereas cytoplasmic CD80 and CD86 were found to a variable extent within all normal donors. Surprisingly, we also found several other neutrophil cytoplasmic CD antigens more commonly associated with B cells, i.e. CD20, CD21 (CR2/EBV-R) and CD22 (BL-CAM). All of these antigens were confined to the "resting" cell cytoplasm and were never found to be expressed on the cell surface. To exclude the possibility that these antigens were absorbed from plasma and to provide evidence for active synthesis, we used a novel whole blood in situ hybridization flow cytometry assay method to detect mRNA specific for these antigens within normal PMNs. We also conducted real-time polymerase chain reactions to confirm these findings using CD22 as a good example of an "inappropriately expressed" CD antigen. These observations therefore provide support for the hypothesis that human PMNs have the potential to express molecules required for antigen presentation and cell signalling.
authors.affiliation: University Department of Pathology, Western Infirmary, Glasgow, G11 6NT, UK. gps1z@clinmed.gla.ac.uk
authors.forename: Gavin P
authors.initials: GP
authors.lastname: Sandilands
authors.forename: Birgit
authors.initials: B
authors.lastname: Hauffe
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Loudon
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Marsh
authors.forename: Anna
authors.initials: A
authors.lastname: Gondowidjojo
authors.forename: Carol
authors.initials: C
authors.lastname: Campbell
authors.forename: Roderick K
authors.initials: RK
authors.lastname: Ferrier
authors.forename: Martina E
authors.initials: ME
authors.lastname: Rodie
mesh_terms: D017951:Antigen Presentation / Q000276:immunology; D015703:Antigens, CD / Q000097:blood* / Q000235:genetics; D000944:Antigens, Differentiation, B-Lymphocyte / Q000097:blood; D015815:Cell Adhesion Molecules; D003593:Cytoplasm / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D037102:Lectins / Q000097:blood; D009504:Neutrophils / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D051918:Sialic Acid Binding Ig-like Lectin 2
chemical_list: D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; C497867:CD22 protein, human; D015815:Cell Adhesion Molecules; D037102:Lectins; D012333:RNA, Messenger; D051918:Sialic Acid Binding Ig-like Lectin 2