title: [The biological effects of recombinant human bone morphogenetic protein 2 on human periodontal ligament fibroblasts].
abstract: OBJECTIVE
  The purpose of this study is to study the biological effects of recombinant human bone morphogenetic protein 2 (rhBMP2) on human periodontal ligament fibroblasts (HPDLFs).
  
  
  METHODS
  Human periodontal ligament fibroblasts were primary cultured and detected the different doses of rhBMP2 on their proliferation, alkaline phosphatase (ALP) activity, osteocalcin (OC) synthesis and formation of the mineralized nodules.
  
  
  RESULTS
  rhBMP2 (0.25-2 mg/ml) had no remarkable effect on the proliferation of HPDLFs. The ALP activity, OC synthesis and formation of the mineralized nodules of HPDLFs were significantly stimulated by 0.5-2 mg/ml rhBMP2.
  
  
  CONCLUSIONS
  The effects of rhBMP2 on HPDLFs are dose-dependent. Not only can rhBMP2 stimulate the expression but also promote the maturation of osteoblastic phenotype of HPDLFs.
authors.affiliation: Ninth People's Hospital, Shanghai Second Medical University.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Si
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000473:pathology*; D006801:Humans; D008297:Male; D008310:Malocclusion / Q000378:metabolism / Q000473:pathology; D015675:Osteocalcin / Q000378:metabolism; D010513:Periodontal Ligament / Q000473:pathology*; D011994:Recombinant Proteins / Q000494:pharmacology; D016212:Transforming Growth Factor beta
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D015675:Osteocalcin; D000469:Alkaline Phosphatase