CD34-positive cells in human umbilical cord blood express nerve growth factor and its specific receptor TrkA.
In this study, we investigated whether hematopoietic stem cells (HSC) and progenitors present in human cord blood can express nerve growth factor (NGF)-specific receptors, TrkA and p75. Our results showed a marked expression of TrkA and NGF in cord blood CD34(+) cells. A gradient of TrkA and NGF expression exists and is highest in cord blood CD34(+) cells, reduced in cord blood mononuclear cells (MNC) and minimal in mononuclear cells isolated from adult peripheral blood. Our findings suggest that NGF may play a role in the differentiation of hematopoietic progenitors and indicate a different requirement for NGF by immune cells, depending on their state of maturity.
Bracci-Laudiero
Luisa
L
Institute of Neurobiology and Molecular Medicine, CNR, Viale Marx 15/43, 00137 Rome, Italy.
Celestino
Domenico
D
Starace
Giuseppe
G
Antonelli
Alessia
A
Lambiase
Alessandro
A
Procoli
Annabella
A
Rumi
Carlo
C
Lai
Marco
M
Picardi
Alessandra
A
Ballatore
Giovanna
G
Bonini
Sergio
S
Aloe
Luigi
L
D000328:Adult; D018952:Antigens, CD34 / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology*; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008875:Middle Aged; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020800:Receptor, Nerve Growth Factor; D020917:Receptor, trkA / Q000235:genetics / Q000378:metabolism*; D017475:Receptors, Nerve Growth Factor / Q000235:genetics / Q000378:metabolism
D018952:Antigens, CD34; D012333:RNA, Messenger; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor; D020932:Nerve Growth Factor; D020917:Receptor, trkA