Chromatographic behavior of packing materials for capillary electrochromatography with a coating of different immobilized polysiloxanes.
Octadecyl silyl silica (ODS) phase coated with immobilized polysiloxanes (OV1701, SE-54, SE-30) were synthesized, their characteristics as capillary electrochromatography (CEC) column packing materials were studied. It was found that, although the polysiloxane coatings were different in polarity, the resulting packing materials showed the highest efficiencies when the respective coating ratios (polysiloxane:ODS, w/w) were all 20-30%. As expected, packing materials coated with different polysiloxanes resulted in different selectivity on solute pairs. Separations on these stationary phases were studied with different factors such as pH values and acetonitrile contents of the mobile phases. It was found that all these kind of stationary phases could resist basic mobile phase with a pH value as high as 11.6. Tests were made to analyze polar, basic drugs with CEC using the stationary phases.
Zhang
Guodong
G
Department of Analytical Chemistry, School of Pharmaceutical Sciences, Peking University, Beijing, China. xbjhfl@263.net
Li
Yongshan
Y
Fang
Yi'ou
Y
Han
Nanyin
N
Xu
Bingjiu
B
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D012833:Siloxanes / Q000737:chemistry*
D012833:Siloxanes