Quantitation of amino acids and amines in the same matrix by high-performance liquid chromatography, either simultaneously or separately.
A literature overview is presented of chromatographic methods currently in use to determine amino acids and mines (i) simultaneously, (ii) in the presence of each other by separate methods, or (iii) amines alone subsequent to their isolation from amino acids. Separation, derivatization and chromatographic conditions are summarized. Advantages and drawbacks of all three possibilities are discussed and criticized in detail.
Molnár-Perl
I
I
Institute of Inorganic and Analytical Chemistry, L. Eötvös University, P.O. Box 32, H-1518 Budapest 112, Hungary. perlne@para.chem.elte.hu
D000588:Amines / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D009764:o-Phthalaldehyde / Q000737:chemistry
D000588:Amines; D000596:Amino Acids; D005456:Fluorescent Dyes; D009764:o-Phthalaldehyde