Discrimination of 13C NMR signals in solid material with liquid-like behavior presenting residual dipolar proton-proton homonuclear interactions: application on seeds.
In this communication, we propose, a modified spin echo fourier transform (SEFT) experiment run under magic angle spinning (MAS) to obtain structural informations of the liquid-like domains inside complex organic materials. It includes a proton-proton dipolar decoupling such as BLEW12 or Lee-Goldburg sequence just after the 180 degrees 13C refocusing pulse and short echo delays are used in order to overcome T(2) relaxation. This very easy implemented sequence allows a clear discrimination among fast relaxing 13C signals between those with a pure liquid-like behavior and those presenting residual proton-proton dipolar coupling. The interests of the sequence, combined with other classical NMR experiments, are illustrated on whole vegetable seeds that represent an example of a complex material.
Bardet
Michel
M
Service de Chimie Inorganique et Biologique, Département de Recherche Fondamentale sur la Matière Condensée, CEA-Grenoble, F-38054 Grenoble Cedex 9, France. bardet@drfmc.ceng.cea.fr
Foray
Marie F
MF
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005583:Fourier Analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011522:Protons; D012639:Seeds / Q000737:chemistry*
D011522:Protons