Effect of D-amino acids on some mitochondrial functions in rat liver.
We studied the role of the D-amino acids (D-aa) D-serine, D-alanine, D-methionine, D-aspartate, D-tyrosine and D-arginine on rat liver mitochondria. The stability of D-amino acids, mitochondrial swelling, transmembrane potential and oxygen consumption were studied under oxidative stress conditions in rat liver mitochondria. In the presence of glutamate-malate all D-aas salts increased mitochondrial swelling, while in the presence of succinate plus rotenone only D-ala, D-arg and D-ser, induced mitochondrial swelling. The transmembrane potential (deltapsi) was decreased in the presence of 1 microM Ca(2+). The D-aas inhibited oxygen consumption in state 3. The D-aa studied exerted effects on mitochondria via an increase of free radicals production.
González-Hernández
J C
JC
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Mich. México.
Aguilera-Aguirre
L
L
Pérez-Vázquez
V
V
Ramírez
J
J
Clemente-Guerrero
M
M
Cortés-Rojo
C
C
Saavedra-Molina
A
A
D000596:Amino Acids / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects*; D008933:Mitochondrial Swelling / Q000187:drug effects; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism
D000596:Amino Acids