title: Effect of hepatic artery flow on bile secretory function after cold ischemia.
abstract: These studies evaluated the influence of hepatic arterial flow on biliary secretion after cold ischemia. Preparation of livers for transplantation or hepatic support impairs biliary secretion. The earliest indication of cold preservation injury during reperfusion is circulatory function. Arterial flow at this time may be critical for bile secretion. Porcine livers were isolated, maintained at 4 degrees for 2 h and connected in an extracorporeal circuit to an anesthetized normal pig. The extracorporeal livers were perfused either by both the hepatic artery and portal vein (dual) or by the portal vein alone (single). Incremental doses of sodium taurocholate were infused into the portal vein of both the dual and single perfused livers, and the bile secretion was compared. Most endogenous bile acids are lost during hepatic isolation. After supplementation, the biliary secretion of phosphatidyl choline and cholesterol was significantly better in the dual than single vessel-perfused livers; however, no difference was seen in bilirubin output. Single perfused livers were completely unable to increase biliary cholesterol in response to bile acid. The dependence of bile cholesterol secretion on arterial flow indicates the importance of this flow to the detoxification of compounds dependent on phosphatidyl choline transport during early transplantation.
authors.affiliation: Department of Surgery, University of Massachusetts Medical School, MA MCP Hahnemann University School of Medicine, Philadelphia, PA, USA.
authors.forename: David P
authors.initials: DP
authors.lastname: Foley
authors.forename: Rocco
authors.initials: R
authors.lastname: Ricciardi
authors.forename: Angela N
authors.initials: AN
authors.lastname: Traylor
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: McLaughlin
authors.forename: Susan E
authors.initials: SE
authors.lastname: Donohue
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Wheeler
authors.forename: William C
authors.initials: WC
authors.lastname: Meyers
authors.forename: Steven H
authors.initials: SH
authors.lastname: Quarfordt
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism*; D001663:Bilirubin / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D015925:Cryopreservation; D005260:Female; D006499:Hepatic Artery / Q000503:physiopathology*; D007511:Ischemia / Q000503:physiopathology*; D008099:Liver / Q000098:blood supply* / Q000187:drug effects; D016031:Liver Transplantation / Q000379:methods / Q000502:physiology; D015427:Reperfusion Injury / Q000503:physiopathology; D013552:Swine; D013656:Taurocholic Acid / Q000494:pharmacology; D014022:Tissue Survival / Q000502:physiology
chemical_list: D013656:Taurocholic Acid; D002784:Cholesterol; D001663:Bilirubin