title: Fast and single solid phase fluorescence spectroscopic batch procedure for (acetyl) salicylic acid determination in drug formulations.
abstract: A solid phase fluorescence spectroscopic batch procedure for (acetyl) salicylic acid in drug formulations have been developed. The procedure is based on the sorption of salicylic acid (SA) on Sephadex DEAE A-25 anion exchanger gel (100 mg) by equilibration from an aqueous solution (10 or 25 ml) for 5 min; the equilibrated gel is transferred into an 1 mm quartz cell and the native fluorescence of SA sorbed on it is directly measured (lambda(ex)=297 nm; lambda(em)=405 nm). Good linearity was found in the 10-200 and 5-100 microg l(-1) ranges (for 10 and 25 ml sample volume, respectively) with R.S.D. (%) of 2.8 and 1.1. The procedure was successfully applied to the determination of acetyl salicylic acid (ASA) in drug formulations after alkaline hydrolysis to yield SA.
authors.affiliation: Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Paraje Las Lagunillas, E-23071 Jaén, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Ortega Algar
authors.forename: N
authors.initials: N
authors.lastname: Ramos Martos
authors.forename: A
authors.initials: A
authors.lastname: Molina Díaz
mesh_terms: D000465:Algorithms; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D001241:Aspirin / Q000032:analysis*; D002110:Caffeine / Q000737:chemistry; D002214:Capsules; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D003061:Codeine / Q000737:chemistry; D004338:Drug Combinations; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D013050:Spectrometry, Fluorescence; D013607:Tablets
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D002214:Capsules; D004338:Drug Combinations; D007202:Indicators and Reagents; D013607:Tablets; D002110:Caffeine; D001241:Aspirin; D003061:Codeine