Water mobility in multicomponent model media as studied by 2H and 17O NMR.
Molecular mobility of water was studied in a microbiological media containing complex and heterogeneous mixtures of cellulose, l-sorbose, and orange serum broth (OSB) using (2)H and (17)O high-resolution NMR. All mixtures showed Lorentian (17)O NMR spectra but complex (2)H NMR line shapes. Sorbose, when solubilized, caused line-narrowing where as cellulose-OSB mixtures showed wide peaks with flat plateaus. Presence of liquid or solvent water had a profound effect on a marked increase in T(2) relaxation time observed in sorbose-containing samples. (17)O NMR data were not composition dependent, while (2)H NMR data were highly sorbose dependent.
Vittadini
Elena
E
Department of Food Science, University of Massachusetts, Amherst 01003, USA.
Dickinson
L Charles
LC
Lavoie
James P
JP
Pham
Xuyen
X
Chinachoti
Pavinee
P
D002482:Cellulose / Q000032:analysis / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003470:Culture Media / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D012995:Solubility; D013013:Sorbose / Q000032:analysis / Q000737:chemistry; D014867:Water / Q000737:chemistry*
D003470:Culture Media; D014867:Water; D002482:Cellulose; D013013:Sorbose