title: [PGM1 genotyping by PCR-SSCP].
abstract: OBJECTIVE
  PGM1 genotyping by PCR-SSCP analysis.
  
  
  METHODS
  Amplified genome DNA from 156 unrelated Han individuals living in Wuhan, PCR products for exon 4 and exon 8 of PGM1 were then analyzed by SSCP to detect the genotypes.
  
  
  RESULTS
  2 alleles and 3 genotypes were detected in exon 4 and 8 respectively. The discrimination power was 0.7318. PCR-SSCP analysis was suitable for determination of PGM1 genotypes from old blood and semen stains.
  
  
  CONCLUSIONS
  PGM1 system typed by PCR-SSCP is useful for forensic identification.
authors.affiliation: Department of Forensic Medicine, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030.
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Song
authors.forename: Qing-en
authors.initials: QE
authors.lastname: Yang
authors.forename: Chun-ying
authors.initials: CY
authors.lastname: Yu
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002681:China; D004247:DNA / Q000032:analysis / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D010733:Phosphoglucomutase / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D012680:Sensitivity and Specificity
chemical_list: D004247:DNA; D010733:Phosphoglucomutase