Serotonin catabolism depends upon location of release: characterization of sulfated and gamma-glutamylated serotonin metabolites in Aplysia californica.
Serotonin is a vital neurotransmitter for the functioning of the nervous system in species throughout the animal phyla. Despite its ubiquitous nature, the metabolism of this molecule has yet to be completely elucidated in even the most basic of organisms. Two novel serotonin catabolites, serotonin-O-sulfate and gamma-glu-serotonin-O-sulfate, are chemically characterized using capillary electrophoresis with wavelength-resolved fluorescence detection and electrospray mass spectrometry, and the formation of gamma-glu-serotonin in Aplysia californica is confirmed. These novel compounds appear to be synthesized enzymatically, and known mammalian enzymes exist for all serotonin transformations observed here. The pathway of serotonin inactivation depends upon the type of neuronal tissue subjected to neurotransmitter incubation, with assorted serotonin products observed in distinct locations. Initially demonstrated to be in the metacerebral cell (MCC) soma, the new serotonin metabolite serotonin-O-sulfate may contribute to important functions in the serotonergic system beyond simple serotonin inactivation.
Stuart
Jeffrey N
JN
Department of Chemistry, University of Illinois, Urbana, Illinois 61801, USA.
Zhang
Xin
X
Jakubowski
Jennifer A
JA
Romanova
Elena V
EV
Sweedler
Jonathan V
JV
D000818:Animals; D001048:Aplysia; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D018698:Glutamic Acid / Q000737:chemistry / Q000378:metabolism*; D006897:Hydroxyindoleacetic Acid / Q000032:analysis / Q000378:metabolism; D066298:In Vitro Techniques; D009928:Organ Specificity; D012701:Serotonin / Q000031:analogs & derivatives* / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013431:Sulfates / Q000378:metabolism
D013431:Sulfates; C033396:5-hydroxytryptamine O-sulfate; D012701:Serotonin; D018698:Glutamic Acid; D006897:Hydroxyindoleacetic Acid