A functional genetic approach suggests a novel interaction between the human immunodeficiency virus type 1 (HIV-1) Tat protein and HIV-1 TAR RNA in vivo.
Human immunodeficiency virus type 1 (HIV-1) Tat and human Cyclin T1 form a complex and together recognize the viral TAR RNA element with specificity. Using HIV-1/equine infectious anaemia virus TAR chimeras, we show that in addition to the well-characterized interaction with the bulge, Tat recognizes the distal stem and the loop of TAR. These data support previously proposed, but unproven, molecular models.
Lund
Lars H
LH
Medicine, Duke University Medical Center, Box 3053 (424 CARL), Research Drive, Durham, NC 27710, USA.|Departments of Molecular Genetics and Microbiology, Duke University Medical Center, Box 3053 (424 CARL), Research Drive, Durham, NC 27710, USA.|Microbiology and Tumor Biology Center and Swedish Institute for Infectious Disease Control, Karolinska Institute, Solna 17182, Sweden.
Wahren
Britta
B
Microbiology and Tumor Biology Center and Swedish Institute for Infectious Disease Control, Karolinska Institute, Solna 17182, Sweden.
Garcia-Blanco
Mariano A
MA
Medicine, Duke University Medical Center, Box 3053 (424 CARL), Research Drive, Durham, NC 27710, USA.|Departments of Molecular Genetics and Microbiology, Duke University Medical Center, Box 3053 (424 CARL), Research Drive, Durham, NC 27710, USA.
D000818:Animals; D002460:Cell Line; D056906:Cyclin T; D016213:Cyclins / Q000378:metabolism*; D004285:Dogs; D015696:Gene Products, tat / Q000235:genetics / Q000378:metabolism*; D016325:HIV Long Terminal Repeat / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D004860:Infectious Anemia Virus, Equine / Q000235:genetics; D008958:Models, Molecular; D011485:Protein Binding; D012367:RNA, Viral / Q000378:metabolism; D011995:Recombination, Genetic; D015533:Transcriptional Activation; D054322:tat Gene Products, Human Immunodeficiency Virus
C503684:CCNT1 protein, human; D056906:Cyclin T; D016213:Cyclins; D015696:Gene Products, tat; D012367:RNA, Viral; D054322:tat Gene Products, Human Immunodeficiency Virus