HPLC-NMR/HPLC-MS analysis of the bark extract of Stauranthus perforatus.
A combination of HPLC-MS and HPLC-NMR techniques has been used to analyse the cytotoxic fractions of the dichloromethane extract of bark of Stauranthus perforatus. Six furanocoumarins (byakangelicol, heraclenin, heraclenol, imperatorin, isopimpinellin and xanthotoxin) and nine quinoline alkaloids (two known compounds, veprisine and 5-hydroxy-1-methyl-2-phenyl-4-quinolone, along with seven novel compounds, stauranthine, 3',4'-dihydroxy-3',4'-dihydroveprisine, 3',4'-dihydroxy-3',4'-dihydrostauranthine, 3',6'-dihydroxy-3',6'-dihydroveprisine, 3',6'-dihydroxy-3',6'-dihydrostauranthine, 6'-hydroxy-3'-ketoveprisine and 6'-hydroxy-3'-ketostauranthine) have been identified in the fractions.
Setzer
William N
WN
Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899, USA. wsetzer@chemistry.uah.edu
Vogler
Bernhard
B
Bates
Robert B
RB
Schmidt
Jennifer M
JM
Dicus
Christopher W
CW
Nakkiew
Pichaya
P
Haber
William A
WA
D000470:Alkaloids / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D011564:Furocoumarins / Q000032:analysis / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011804:Quinolines / Q000032:analysis / Q000737:chemistry; D027821:Rutaceae / Q000737:chemistry*
D000470:Alkaloids; D011564:Furocoumarins; D010936:Plant Extracts; D011804:Quinolines