Detection of progesterone in whole blood samples.
The progesterone concentration in blood samples can be utilised as a marker for the diagnosis of early pregnancy, endocrinopathy and virilism. Here, we describe a method for progesterone detection and measurement in whole blood samples by a surface sensitive biosensor used in conjunction with an integrated optical grating coupler. This device determines refractive index changes near the biosensor's surface. Hence, biological species bound to a surface layer can be measured in real-time without any label. For the measurements, we have modified the indirect competitive immunoassay principle. The concentration of the progesterone antibody was kept at 1 microg/ml. Progesterone concentration was determined in buffer solution and whole blood in a range between 0.005 and 10 ng/ml. The detection limit was determined to be 3 pM. The relative standard deviation was calculated to be 3.5%.
Ehrentreich-Förster
Eva
E
Department Molecular Bioanalytics, Fraunhofer Institute for Biomedical Engineering, Arthur-Scheunert-Allee 114-116, 14558 Bergholz-Rehbrücke, Germany. eva.ehrentreich@ibmt.fhg.de
Scheller
Frieder W
FW
Bier
Frank F
FF
D000936:Antigen-Antibody Complex / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods*; D011374:Progesterone / Q000097:blood* / Q000276:immunology; D012031:Refractometry / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000936:Antigen-Antibody Complex; D011374:Progesterone