title: Functions of malonate decarboxylase subunits from Pseudomonas putida.
abstract: Malonate decarboxylase from Pseudomonasputida is composed of five subunits, alpha, beta, gamma, delta, and epsilon. Two subunits, delta and epsilon, have been identified as an acyl-carrier protein (ACP) and malonyl-CoA:ACP transacylase, respectively. Functions of the other three subunits have not been identified, because recombinant subunits expressed in Escherichia coi formed inclusion bodies. To resolve this problem, we used a coexpression system with GroEL/ES from E. coli, and obtained active recombinant subunits. Enzymatic analysis of the purified recombinant subunits showed that the alpha subunit was an acetyl-S-ACP:malonate ACP transferase and that the betagamma-subunit complex was a malonyl-S-ACP decarboxylase.
authors.affiliation: Department of Bioresource Science, College of Agriculture, Ibaraki University, 3-21-1 Chuo, Ami, Ibaraki 300-0393, Japan. chohnan@mx.ibaraki.ac.jp
authors.forename: Shigeru
authors.initials: S
authors.lastname: Chohnan
authors.forename: Kayo
authors.initials: K
authors.lastname: Akagi
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Takamura
mesh_terms: D000213:Acyl Carrier Protein / Q000378:metabolism; D051080:Acyl-Carrier Protein S-Malonyltransferase; D000217:Acyltransferases / Q000737:chemistry / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D029968:Escherichia coli Proteins; D054889:Fatty Acid Synthase, Type II; D010957:Plasmids / Q000235:genetics; D016958:Pseudomonas putida / Q000201:enzymology*; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000213:Acyl Carrier Protein; D017931:DNA Primers; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D000217:Acyltransferases; D051080:Acyl-Carrier Protein S-Malonyltransferase; C494968:fabD protein, E coli; D002262:Carboxy-Lyases; C071852:malonate decarboxylase; D054889:Fatty Acid Synthase, Type II