Phenotype of Pseudomonas aeruginosa isolates causing corneal infection between 1997 and 2000.
OBJECTIVE
To investigate the relationship between functional phenotype of and the associated human corneal infection.


METHODS
This was an experimental pilot study of patients presenting with corneal infections at the Jules Stein Eye Institute with presumed infection during the period from 12/30/97 to 9/1/00. Thirteen patients were admitted to the study based on positive identification of the causative pathogen as and patient consent. Data were collected (including bacterial cultures, lens wear schedule and care, gender and age, completed history questionnaire, clinical photographs). Statistical analysis of possible correlations was performed. Phenotypes of were determined, and clinical factors associated with infection were explored.


RESULTS
Both invasive and cytotoxic phenotypes of were isolated in equal proportion. Cytotoxic strains and invasive strains were found to be associated with patients younger than 50 years of age and older than 50 years of age, respectively.


CONCLUSIONS
remains a significant pathogen in corneal infection, especially during contact lens wear. The age of the patient may influence the phenotype of causing infection. Since invasive and cytotoxic strains have different effects on corneal cells, treatment of the infection might require different approaches depending on this phenotype of the causative bacteria.
Cowell
Brigitte A
BA
School of Optometry, University of California-Berkeley, Berkeley, CA 94720-2020, USA.
Weissman
Barry A
BA
Yeung
Karen K
KK
Johnson
Larisa
L
Ho
Sharon
S
Van
Rajana
R
Bruckner
David
D
Mondino
Bartly
B
Fleiszig
Suzanne M J
SM
D000293:Adolescent; D000328:Adult; D015373:Bacterial Typing Techniques; D002648:Child; D002675:Child, Preschool; D003316:Corneal Diseases / Q000382:microbiology*; D015818:Eye Infections, Bacterial / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000302:isolation & purification