Isoflavone aglycon produced by culture of soybean extracts with basidiomycetes and its anti-angiogenic activity.
Soybean extracts (SBE) containing isoflavone glycosides were cultured with Ganoderma lucidum mycelia producing beta-glucosidase. The anti-angiogenic effects of the cultivated product, containing rich in genistein, named GCP (genistein combined polysaccharide), were assessed with chick chorioallantoic membranes (CAM) and a mouse dorsal air-sac model. Beta-glucosidase produced by the mycelia converted the isoflavone glycosides into aglycons. A test of volunteers showed that serum concentrations of genistein in the subjects treated with GCP (n = 4) at 3 h after administration were significantly higher than those in the subjects treated with SBE (n = 4). GCP inhibited angiogenesis in CAM, and the activity of GCP was greater than that of SBE. GCP inhibited the formation of new vessels induced by colon carcinoma cells in vivo.
Miura
Takehito
T
Department of Biochemistry, Amino Up Chemical Co., Ltd., 363-32 Shin-ei, Kiyota-ku, Sapporo 004-0839, Japan. au_labo@aminoup.co.jp
Yuan
Lan
L
Sun
Buxiang
B
Fujii
Hajime
H
Yoshida
Mayumi
M
Wakame
Koji
K
Kosuna
Ken-ichi
K
D000328:Adult; D000400:Air Sacs / Q000098:blood supply / Q000187:drug effects; D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D002642:Chick Embryo; D003110:Colonic Neoplasms / Q000098:blood supply / Q000188:drug therapy; D005260:Female; D019833:Genistein / Q000097:blood / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology* / Q000627:therapeutic use; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D020940:Reishi / Q000737:chemistry* / Q000201:enzymology; D013025:Glycine max / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D001617:beta-Glucosidase / Q000378:metabolism
D020533:Angiogenesis Inhibitors; D007529:Isoflavones; D010936:Plant Extracts; D011134:Polysaccharides; C473311:genistein combined polysaccharide; D019833:Genistein; D001617:beta-Glucosidase