New approach to the simultaneous analysis of catecholamines and tyrosines in biological fluids.
New high-performance liquid chromatographic (HPLC) methods with amperometric-CoulArray detection were developed for simultaneous analyses of norepinephrine, epinephrine, L-DOPA, dopamine, 3-nitrotyrosine, m-, o-, and p-tyrosines. Overall, detection limit was in the low pmol range with amperometry, and in the low fmol range for the CoulArray method. Linear (r2 = 0.99) detector performances were observed in the ranges of 2-200 pmol with amperometry, and 0.2-20 pmol for the CoulArray method. Analytical precision values were better than 80 and 95% for HPLC-amperometry and HPLC-CoulArray method, respectively. These methods offer sensitivity, specificity, minimal sample requirement, and especially the HPLC-CoulArray method allows simultaneous assessment of various similar biomolecules.
Kumarathasan
Prem
P
Inhalation Toxicology Section, Safe Environments Programme, Healthy Environments and Consumer Safety Branch, Health Canada, Environmental Health Centre, 0803C Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2. premkumari_kumarathasan@hc-sc.gc.ca
Vincent
Renaud
R
D000818:Animals; D002395:Catecholamines / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014443:Tyrosine / Q000097:blood*
D002395:Catecholamines; D014443:Tyrosine