title: Screening and characterization of human single-chain Fv antibody against beta-amyloid peptide 40.
abstract: Expression of single-chain variable fragment antibody on the surface of filamentous bacteriophage is widely used to make antibodies with pre-defined specificities. Using direct selection on solid phase-bound amyloid-beta 40 peptide, which is a main pathogenic feature of Alzheimer's disease, we obtained single-chain Fv antibody with specific binding activity. The binding epitope of the antibody is located between amino acids 1 and 16 of the peptide antigen. DNA sequencing showed that the gene coding for the single chain Fv antibody consists of 768 bp, and the complementarity-determining regions were deduced.
authors.affiliation: Department of Nuclear Medicine, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Science, Beijing, China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Cai
authors.forename: Shizhen
authors.initials: S
authors.lastname: Wang
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Zhong
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.forename: Zhijuan
authors.initials: Z
authors.lastname: Ji
authors.forename: Shuli
authors.initials: S
authors.lastname: Sheng
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D017104:Bacteriophage M13 / Q000235:genetics; D015723:Gene Library; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
chemical_list: D016229:Amyloid beta-Peptides; D007135:Immunoglobulin Variable Region; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40)