Correlation between active and passive isometric force and intramuscular pressure in the isolated rabbit tibialis anterior muscle.
The purpose of this study was to quantify the relationship between intramuscular pressure (IMP) and muscle force during isometric muscle contraction of the rabbit tibialis anterior (TA) absent the effect of either bone or fascia. To quantify this relationship, length-tension experiments were performed on the isolated TA of the New Zealand White rabbit (mass=2.5+/-0.5kg, n=12). The knee was fixed in a custom jig, the distal tendon of the TA was attached to a servomotor, and a 360 microm fiber optic pressure transducer was inserted into the TA. The peroneal nerve was stimulated to define optimal length (L(0)). The length-tension curve was created using 40Hz isometric contractions with 2-min rest intervals between each contraction. Measurements began at L(0)-50%L(f) and progressed to L(0)+50%L(f), changing the length-tension in 5% L(f) increments after each contraction. Qualitatively, the length-tension curve for isometric contractions was mimicked by the length-pressure curve for both active and passive conditions. Linear regression was performed individually for each animal for the ascending and descending limb of the length-tension curve and for active and passive conditions. Pressure-force coefficients of determination ranged from 0.138-0.963 for the active ascending limb and 0.343-0.947 for the active descending limb. Passive pressure coefficients of determination ranged from 0.045-0.842 for the ascending limb and 0.672-0.982 for the descending limb. These data indicate that IMP measurement provide a fairly accurate index of relative muscle force, especially at muscle lengths longer than optimal.
Davis
Jennifer
J
Department of Orthopaedics and Bioengineering (9151), Veterans Administration Medical Center, San Diego School of Medicine, University of California, 3350 La Jolla Village Drive, San Diego, CA 92161, USA. rlieber@ucsd.edu
Kaufman
Kenton R
KR
Lieber
Richard L
RL
D000818:Animals; D000843:Ankle Joint / Q000502:physiology; D004548:Elasticity; D004558:Electric Stimulation; D007537:Isometric Contraction / Q000502:physiology*; D009038:Motion; D018482:Muscle, Skeletal / Q000294:innervation / Q000502:physiology* / Q000601:surgery; D010543:Peroneal Nerve / Q000502:physiology; D011312:Pressure; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013223:Statistics as Topic; D013314:Stress, Mechanical