title: Determination of organic peroxides by liquid chromatography with on-line post-column ultraviolet irradiation and peroxyoxalate chemiluminescence detection.
abstract: A HPLC method was developed for the simultaneous determination of organic peroxides and hydrogen peroxide with peroxyoxalate chemiluminescence (PO-CL) detection following on-line UV irradiation. Organic peroxides [i.e., benzoyl peroxide (BP), tert.-butyl hydroperoxide (BHP), tert.-butyl perbenzoate (BPB), cumene hydroperoxide (CHP)] were UV irradiated (254 nm, 15 W) to generate hydrogen peroxide, which was determined by PO-CL detection. The conditions for UV irradiation and PO-CL detection were optimized by a flow injection analysis (FIA) system. Generation of hydrogen peroxide from peroxides with on-line UV irradiation also was confirmed by the FIA system by incorporating an enzyme column reactor immobilized with catalase. The separation of four organic peroxides and hydrogen peroxide by HPLC was accomplished isocratically on an ODS column within 30 min. The detection limits (signal-to-noise ratio=3) were 1.1 microM for hydrogen peroxide, 6.8 microM for BP, 31.3 microM for BHP, 7.5 microM for BPB and 1.3 microM for CHP. The proposed method was applied to the determination of BP in wheat flour.
authors.affiliation: Faculty of Education and Human Studies, Akita University, 1-1 Tegatagakuen-machi, Akita 010-8502, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Wada
authors.forename: Keiyu
authors.initials: K
authors.lastname: Inoue
authors.forename: Ayuko
authors.initials: A
authors.lastname: Thara
authors.forename: Naoya
authors.initials: N
authors.lastname: Kishikawa
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Nakashima
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008163:Luminescent Measurements; D009930:Organic Chemicals / Q000032:analysis*; D010070:Oxalates / Q000737:chemistry*; D010545:Peroxides / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009930:Organic Chemicals; D010070:Oxalates; D010545:Peroxides; C044622:peroxyoxalate