title: A novel cellulolytic, anaerobic, and thermophilic bacterium, Moorella sp. strain F21.
abstract: A cellulolytic and thermophilic anaerobe was isolated from soil. This bacterium made a halo on a roll-tube culture containing Avicel. Analysis of the PCR-based 16S rRNA gene sequence showed that the bacterium was closely related to Moorella thermoacetica. Scanning electron microscopy showed the bacterium is a rod and has no protuberant structure on the surface of cells growing on cellulose, suggesting that this strain is a non-cellulosomal cellulolytic bacterium. Carboxymethyl cellulase and xylanase activities were detected in the culture broth. A major fermentation product from ball-milled cellulose was acetate. This strain has a potential to convert cellulosic biomass to acetate, directly.
authors.affiliation: Department of Sustainable Resource Science, Faculty of Bioresources, School of Medicine, Mie University, Tsu 514-8507, Japan. karita@bio.mie-u.ac.jp
authors.forename: Shuichi
authors.initials: S
authors.lastname: Karita
authors.forename: Kengo
authors.initials: K
authors.lastname: Nakayama
authors.forename: Masakazu
authors.initials: M
authors.lastname: Goto
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakka
authors.forename: Wan-Jae
authors.initials: WJ
authors.lastname: Kim
authors.forename: Satoru
authors.initials: S
authors.lastname: Ogawa
mesh_terms: D001421:Bacteria, Anaerobic / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D018533:Biomass; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D005285:Fermentation; D005798:Genes, Bacterial / Q000235:genetics; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000378:metabolism
chemical_list: D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002482:Cellulose; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase