title: Bone tissue reconstruction using titanium fiber mesh combined with rat bone marrow stromal cells.
abstract: The study aim was to evaluate the effect of bone marrow stromal cells (BMSCs) cultured in titanium fiber mesh and implanted in a rat cranial defect. A total of 24 titanium meshes were placed in a tube containing 10 ml BMSC suspension (3 x 10(6)cells/ml) and the tube was rotated on a rotation plate (2 rpm) during 3 h. Thereafter, meshes with cells were subcultured for 1 day under standard conditions. Cell-loaded implants and non-cell-loaded controls were placed in a 8 mm cranial defect and retrieved after 3, 15 and 30 days of implantation. Histology showed that after 3 days of implantation, the mesh porosity of both implant groups was mainly invaded with blood cells. On the other hand, at 15 days of implantation, the cell-loaded implants were filled for 15 +/- 10% of their volume with bone, while the controls showed 1.5 +/- 3.5% of bone. The 30-day cell-loaded implants showed 40 +/- 12.5% of bone and the 30-day control implants 17 +/- 14.5%. At both implantation times the differences were statistically significant. Therefore, we conclude that inoculation of titanium fiber mesh with BMSCs can improve the bone healing capacity of this material.
authors.affiliation: Department of Biomaterials, College of Dental Science, University Medical Center Nijmegen, PO Box 9101, Nijmegen 6500 HB, Netherlands.
authors.forename: Juliette
authors.initials: J
authors.lastname: van den Dolder
authors.forename: Edward
authors.initials: E
authors.lastname: Farber
authors.forename: Paul H M
authors.initials: PH
authors.lastname: Spauwen
authors.forename: John A
authors.initials: JA
authors.lastname: Jansen
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D016026:Bone Marrow Transplantation; D001842:Bone and Bones / Q000166:cytology* / Q000601:surgery*; D002478:Cells, Cultured; D008297:Male; D008422:Materials Testing; D016348:Osseointegration; D010012:Osteogenesis; D019736:Prostheses and Implants; D051381:Rats; D011916:Rats, Inbred F344; D017154:Stromal Cells / Q000637:transplantation*; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D014025:Titanium
chemical_list: D001672:Biocompatible Materials; D014025:Titanium