Experimental Van Deemter plots of shear-driven liquid chromatographic separations in disposable microchannels.
We present a new stationary phase coating method, yielding a monolayer of densely arrayed porous HPLC beads (d(p)=4 microm) for use in a disposable shear-driven flow LC system. The system is inherently suited for whole-column detection through the small voids between the individual particles of the layer. The chromatographic performance of the system has been characterized by performing a series of coumarin dye separation experiments (reversed-phase mode) and by measuring the theoretical plate height as a function of the mobile phase velocity. The resulting Van Deemter curve, yielding a value of about 90,000 plates/m near the u=u(opt) velocity, shows good agreement with the theoretical expectations, and hence constitutes the first full validation of the theory of shear-driven chromatography.
Vervoort
Nico
N
Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium. nvervoor@vub.ac.be
Clicq
David
D
Baron
Gino V
GV
Desmet
Gert
G
D002853:Chromatography, Liquid / Q000379:methods*; D008855:Microscopy, Electron, Scanning