Successful ex vivo normothermic liver perfusion with purely artificial products using artificial blood.
We tried to make an ex vivo functioning liver with an artificial perfusate that consisted of artificial blood in the pig liver. A liver graft from a female pig weighing 20 kg was harvested in the usual manner. The perfusion solution consisted of artificial blood, L-15 medium, distilled water, bovine serum albumin, NaHCO3, NaOH, KCl, human regular insulin, 50% glucose solution, and dexamethasone. The isolated liver was perfused with this oxygenated perfusate through the portal vein at a rate of 300 ml/min for 9 hours. Seven livers were perfused for 9 hours in this system. Five of the livers showed mean oxygen consumption of over 8 ml-O2/min during perfusion. Histological findings showed that the hepatic architecture was almost completely preserved and numerous hepatocytes exhibited PAS-positive cytoplasmic glycogen deposits in these livers. These observations indicate that we have succeeded in developing an ex vivo functioning liver with an artificial perfusate employing artificial blood.
Nui
A
A
First Department of Surgery, Sapporo Medical University, School of Medicine, Sapporo, Japan. katsuram@sapmed.ac.jp
Katsuramaki
T
T
Kikuchi
H
H
Kukita
K
K
Nagayama
M
M
Meguro
M
M
Kimura
H
H
Isobe
M
M
Hirata
K
K
D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology; D005260:Female; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D066298:In Vitro Techniques; D008099:Liver / Q000098:blood supply* / Q000166:cytology / Q000187:drug effects; D008102:Liver Circulation; D010101:Oxygen Consumption / Q000187:drug effects; D013552:Swine
D001802:Blood Substitutes