The role of lipopolysaccharide in T-cell responses following DNA vaccination.
Bacterial products, including lipopolysaccharide (LPS), are potential impurities in plasmid DNA vaccines. LPS has immunostimulatory properties even at exceedingly low concentrations through activation of Toll-like receptor 4 (TLR4). The potency of T-cell responses after vaccination was tested with DNA containing high LPS or depleted of LPS in TLR4-competent and TLR4-deficient mice. CD8(+) T-cell responses were readily induced in TLR4-deficient mice immunized with DNA depleted of LPS. LPS in DNA vaccines is not required for CD8(+) T-cell responses.
Hawkins
William G
WG
Swim Across America Laboratory, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA.
Trcka
Jiri
J
Segal
Neil
N
Blachere
Nathalie E
NE
Gold
Jason S
JS
Moroi
Yoichi
Y
Bowne
Wilbur B
WB
Lewis
Jonathan J
JJ
Wolchok
Jedd D
JD
Houghton
Alan N
AN
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003713:Dendritic Cells / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011956:Receptors, Cell Surface / Q000502:physiology; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*
D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors; D019444:Vaccines, DNA