Separation of megabased-sized DNA molecules of Aspergillus niger using pulsed field gel electrophoresis.
In this study, the chromosomal DNAs were extracted from Aspergillus niger Z10 wild type strain and these DNAs were separated using the contour clamped homogeneous electric field gel electrophoresis (CHEF) system. This system is laboratory-made and is operated by a computer program. Total DNAs resolved into five distinct chromosomal bands. The size of the chromosomes was estimated as being between 3.3 Mb to 6.4 Mb.
Coral
Gökhan
G
University, Faculty of Arts and Sciences, Department of Biology 33342 Ciftliköy-Mersin, Turkey. coral@mersin.edu.tr
Omer
Colak
C
Unaldi
Mutlu Nisa
MN
D001234:Aspergillus niger / Q000235:genetics*; D015825:Chromosomes, Fungal / Q000737:chemistry* / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000295:instrumentation / Q000379:methods*; D008970:Molecular Weight
D004271:DNA, Fungal