title: Transposition of the Drosophila hydei Minos transposon in the mouse germ line.
abstract: We tested the suitability of the fly transposon Minos, a member of the Tc1/mariner superfamily, for insertional mutagenesis in the mouse germ line. We generated a transgenic mouse line expressing Minos transposase in growing oocytes and another carrying a tandem array of nonautonomous transposons. The frequency of transposition in the progeny derived from oocytes carrying both transgenes is 8.2%. Analysis of the new integration sites shows a high frequency of transpositions to a different chromosome. Thus Minos transposition could be an effective system for insertional mutagenesis and functional genomic analysis in the mouse.
authors.affiliation: Department of Cell Biology and Genetics, Faculty of Medicine, Erasmus University Rotterdam, The Netherlands.
authors.forename: Dubravka
authors.initials: D
authors.lastname: Drabek
authors.forename: Laskaro
authors.initials: L
authors.lastname: Zagoraiou
authors.forename: Ton
authors.initials: T
authors.lastname: deWit
authors.forename: An
authors.initials: A
authors.lastname: Langeveld
authors.forename: Chariklea
authors.initials: C
authors.lastname: Roumpaki
authors.forename: Clio
authors.initials: C
authors.lastname: Mamalaki
authors.forename: Charalambos
authors.initials: C
authors.lastname: Savakis
authors.forename: Frank
authors.initials: F
authors.lastname: Grosveld
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D004251:DNA Transposable Elements; D004330:Drosophila / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D051379:Mice
chemical_list: D004251:DNA Transposable Elements