title: Successful unrelated cord blood transplantation in two children with severe combined immunodeficiency syndrome.
abstract: We describe the successful unrelated cord blood transplantation in two patients affected by a Zap-70 deficiency and an Omenn-like syndrome, respectively. The patients were hospitalised for recurrent infections at the age of 13 and 2 months, respectively. An unrelated cord blood unit was found for each. The conditioning regimen was cyclophosphamide, busulfan and antithymocyte globulin. The total number of infused cells was 15.1 x 10(7)/kg and 17 x 10(7)/kg, respectively. Neutrophil engraftment was achieved on days +15 and +23, and platelet count >50 x 10(9)/l was achieved on days +21 and +52, respectively. One patient presented acute Graft-versus-host disease (GVHD) grade I and the other grade III. Chimerism was mixed and full donor. Normal lymphoproliferative response to mitogens and alloantigens was detectable at 6 months for both. No chronic GVHD was observed in either. The patients are alive and well at 53 and 15 months after transplantation. In conclusion, umbilical cord blood represents a valid alternative source of haemopoietic stem cells.
authors.affiliation: Department of Paediatrics, University of Turin, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Fagioli
authors.forename: E
authors.initials: E
authors.lastname: Biasin
authors.forename: M
authors.initials: M
authors.lastname: Berger
authors.forename: F
authors.initials: F
authors.lastname: Nesi
authors.forename: E H
authors.initials: EH
authors.lastname: Saroglia
authors.forename: R
authors.initials: R
authors.lastname: Miniero
authors.forename: S
authors.initials: S
authors.lastname: Martino
authors.forename: P A
authors.initials: PA
authors.lastname: Tovo
mesh_terms: D000961:Antilymphocyte Serum / Q000627:therapeutic use; D002066:Busulfan / Q000627:therapeutic use; D016572:Cyclosporine / Q000627:therapeutic use; D005312:Fetal Blood; D005759:Gastroenteritis / Q000150:complications / Q000382:microbiology; D006086:Graft vs Host Disease / Q000517:prevention & control; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007223:Infant; D007231:Infant, Newborn; D012480:Salmonella Infections / Q000150:complications; D016511:Severe Combined Immunodeficiency / Q000209:etiology / Q000276:immunology / Q000628:therapy*; D033581:Stem Cell Transplantation / Q000379:methods*; D013601:T-Lymphocytes / Q000276:immunology; D018183:Transplantation Chimera; D019172:Transplantation Conditioning / Q000379:methods; D014184:Transplantation, Homologous; D016896:Treatment Outcome
chemical_list: D000961:Antilymphocyte Serum; D007166:Immunosuppressive Agents; D016572:Cyclosporine; D002066:Busulfan