title: Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides.
abstract: Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls. Recombinant XET from poplar has been purified from a Pichia pastoris expression system and crystallized. Two different crystal forms were obtained by vapour diffusion from potassium sodium tartrate and from an imidazole buffer using sodium acetate as a precipitant. Data were collected from these crystal forms to 3.5 and 2.1 A resolution, respectively. The first crystal form was found to belong to space group P3(1)21 or P3(2)21 (unit-cell parameters a = 98.6, b = 98.6, c = 98.5 A) and the second crystal form to space group P6(3) (unit-cell parameters a = 188.7, b = 188.7, c = 46.1 A).
authors.affiliation: Department of Cell and Molecular Biology, Uppsala University, Box 596, SE-75 124 Uppsala, Sweden. patrik@xray.bmc.uu.se
authors.forename: Patrik
authors.initials: P
authors.lastname: Johansson
authors.forename: Stuart
authors.initials: S
authors.lastname: Denman
authors.forename: Harry
authors.initials: H
authors.lastname: Brumer
authors.forename: Asa M
authors.initials: AM
authors.lastname: Kallas
authors.forename: Hongbin
authors.initials: H
authors.lastname: Henriksson
authors.forename: Terese
authors.initials: T
authors.lastname: Bergfors
authors.forename: Tuula T
authors.initials: TT
authors.lastname: Teeri
authors.forename: T Alwyn
authors.initials: TA
authors.lastname: Jones
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D016695:Glycosyltransferases / Q000737:chemistry* / Q000302:isolation & purification; D007202:Indicators and Reagents; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D014197:Trees / Q000201:enzymology*
chemical_list: D007202:Indicators and Reagents; D011994:Recombinant Proteins; D016695:Glycosyltransferases; C073693:xyloglucan endotransglycosylase