Highly enantioselective synthesis of alpha,alpha-disubstituted malonamic acids through asymmetric hydrolysis of dinitriles with Rhodococcus sp. CGMCC 0497.
Highly enantioselective hydrolysis of alpha,alpha-disubstituted malononitriles by the strain Rhodococcus sp. CGMCC 0497 expressing both nitrile hydratase and amidase activity to give (R)-alpha,alpha-disubstituted malonamic acids which could be converted to valuable (R)- or (S)-alpha-alkylated amino acids are reported and the yields of the products are improved remarkably at a lower reaction temperature.
Wu
Zhong-Liu
ZL
State Key Laboratory of Bio-organic & Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 354 Fenglin Road, Shanghai 200032, China.
Li
Zu-Yi
ZY
D000581:Amidohydrolases / Q000378:metabolism; D006836:Hydro-Lyases / Q000378:metabolism; D006868:Hydrolysis; D008314:Malonates / Q000138:chemical synthesis*; D009570:Nitriles / Q000737:chemistry; D012240:Rhodococcus / Q000201:enzymology / Q000378:metabolism*; D013237:Stereoisomerism
D008314:Malonates; D009570:Nitriles; D000581:Amidohydrolases; C019665:amidase; D006836:Hydro-Lyases; C048831:nitrile hydratase