title: Pregnancies after ICSI using sperm with abnormal head-tail junction from two brothers: case report.
abstract: We report ICSI pregnancies in two couples with a history of long standing primary infertility in which the sperm of the male partner were either acephalic or had abnormal head-midpiece attachments. The two couples, in which the men are brothers, underwent ICSI. Sperm were analysed by transmission electron microscopy and immunocytochemistry with an anti-MPM2 monoclonal antibody. The first couple underwent two ICSI cycles, each consisting of the injection of two mature oocytes and the transfer of two embryos. A successful pregnancy occurred after the second transfer and led to the birth to a healthy girl. The second couple underwent three ICSI cycles, each consisting of the injection of 18 oocytes and the transfer of two embryos; the last of these led to a triple ongoing pregnancy which included two identical twins. Caesarean section led to the birth of three fetal-growth restricted children. This case report demonstrates that ongoing pregnancies can be achieved in cases of abnormal development of the head-neck attachment. The genetic origin of this syndrome is generally accepted, but the phenotypic heterogeneity observed by light and electron microscopy among published cases suggests that there are a variety of genetic causes of this syndrome.
authors.affiliation: Centre de Procréation Médicalement Assistée, AP-HM, Hôpital de la Conception, 147 Boulevard Baille, 13385 Marseille cedex 05, France.
authors.forename: G
authors.initials: G
authors.lastname: Porcu
authors.forename: G
authors.initials: G
authors.lastname: Mercier
authors.forename: P
authors.initials: P
authors.lastname: Boyer
authors.forename: V
authors.initials: V
authors.lastname: Achard
authors.forename: J
authors.initials: J
authors.lastname: Banet
authors.forename: M
authors.initials: M
authors.lastname: Vasserot
authors.forename: C
authors.initials: C
authors.lastname: Melone
authors.forename: J
authors.initials: J
authors.lastname: Saias-Magnan
authors.forename: C
authors.initials: C
authors.lastname: D'Ercole
authors.forename: C
authors.initials: C
authors.lastname: Chau
authors.forename: M R
authors.initials: MR
authors.lastname: Guichaoua
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D018797:Cell Cycle Proteins; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007248:Infertility, Male / Q000235:genetics* / Q000378:metabolism / Q000628:therapy*; D016547:Kinesins; D008297:Male; D008854:Microscopy, Electron; D010750:Phosphoproteins / Q000378:metabolism; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D011272:Pregnancy, Multiple; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000002:abnormalities* / Q000648:ultrastructure; D014310:Triplets
chemical_list: D000911:Antibodies, Monoclonal; D018797:Cell Cycle Proteins; D010750:Phosphoproteins; C438388:KIF20B protein, human; D016547:Kinesins