A cathepsin B-like cysteine protease gene from Hordeum vulgare (gene CatB) induced by GA in aleurone cells is under circadian control in leaves.
A barley cDNA clone encoding a putative cysteine protease with sequence homology to cathepsin B from mammalian cells has been characterized. This barley gene (CatB) is ubiquitously expressed, its mRNA being detected in leaves and roots, immature, mature and germinating embryos, in developing endosperms, and in aleurones upon germination, as assessed by northern blot analysis. The CatB mRNA expression in leaves increased by cold shock (6 degrees C), was not affected by wounding, and was under circadian control. These transcripts increased in the aleurone upon germination, whereas those for a cystatin encoding gene (Icy), that inhibits commercial cathepsin B in vitro, decreased. Gibberellin (GA) treatment of isolated barley aleurones induced and abscisic acid (ABA) repressed the steady-state levels of CatB mRNA, while Icy expression had an opposite pattern of mRNA accumulation in aleurones treated with GA. No response to GA or ABA was detected in leaves.
Martínez
Manuel
M
Laboratorio de Bioquímica y Biología Molecular, Dpto de Biotecnología, ETSI Agrónomos-UPM, Ciudad Universitaria s/n, 28040 Madrid, Spain.
Rubio-Somoza
Ignacio
I
Carbonero
Pilar
P
Díaz
Isabel
I
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002401:Cathepsin B / Q000235:genetics* / Q000378:metabolism; D002940:Circadian Rhythm / Q000502:physiology*; D003001:Cloning, Molecular; D003080:Cold Temperature; D003546:Cysteine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000528:radiation effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D005875:Gibberellins / Q000494:pharmacology*; D001467:Hordeum / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D008027:Light; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000201:enzymology* / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012639:Seeds / Q000201:enzymology* / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D005875:Gibberellins; D010940:Plant Proteins; D000040:Abscisic Acid; D003546:Cysteine Endopeptidases; D002401:Cathepsin B