Effect of a chayotte (Sechium edule) extract on the labeling of red blood cells and plasma proteins with technetium-99m: in vitro and in vivo studies.
Sechium edule (chayotte) is used as food or as medication in popular medicine. The labeling of blood elements with technetium-99m (99mTc) has been altered by drugs (synthetic and natural). Some authors have reported biological effects concerning the chayotte. We have evaluated the influence of chayotte extracts (macerated and infusion) on the labeling of blood elements with 99mTc. In vitro study, blood was incubated with the extracts, (6.25, 12.5, 25, 50 and 100% v/v). In in vivo study, the animals were treated with the extracts (100% v/v), as drinking water (15 and 60 days) and samples of blood were withdrawn. The blood samples were incubated with stannous chloride and with 99mTc. Plasma (P) and blood cells (BC) were isolated, also precipitated with trichloroacetic acid and soluble (SF) and insoluble fractions (IF) separated. There was a (p < 0.05) decrease in the radioactivity in BC, IF-BC and IF-P with the infusion (100%) and a slight decrease in the uptake of 99mTc by BC and a strong decrease in the fixation in IF-P with the macerated when the extracts were administrated in vivo (15 days). In 60 days, there was a decrease in BC (98.77 to 53.53%), in IF-BC (90.36 to 21.20%) and in IF-P (77.20 to 11.01%). In vitro study no alterations on the labeling of blood elements were found, however, we have found alterations on the fixation of 99mTc in the in vivo study, probably, due to the metabolization of chayotte capable to induce the generation of active metabolites.
Feliciano
Gláucio Diré
GD
Universidade do Estado do Rio de Janeiro, Instituto de Biologia, Departamento de Biofísica e Biometria, Av. 28 de Setembro, 87, CEP 20551-030, Rio de Janeiro, RJ, Brazil.
Lima
Elaine Alves Correia
EA
Pereira
Mario José dos Santos
MJ
de Oliveira
Márcia Betânia Nunes
MB
Moreno
Silvana Ramos Farias
SR
de Mattos
Deise Mara Machado
DM
Levi Jales
Roberto
R
Bernardo-Filho
Mario
M
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D019662:Cucurbitaceae / Q000737:chemistry*; D004912:Erythrocytes / Q000000981:diagnostic imaging* / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D010936:Plant Extracts / Q000494:pharmacology; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D013667:Technetium / Q000097:blood*
D001798:Blood Proteins; D010936:Plant Extracts; D019275:Radiopharmaceuticals; D013667:Technetium