title: Molecular identification and antibody testing of Chlamydophila pneumoniae in a subgroup of patients with HIV-associated dementia complex. Preliminary results.
abstract: Chlamydophila pneumoniae DNA was investigated by polymerase chain reaction (PCR), in cerebrospinal fluid (CSF) specimens from patients suffering from HIV-1-associated dementia complex (HADC). Four (17.3%) cases of C. pneumoniae infection were identified among 23 HADC individuals with DNA amplification of major outer membrane protein (MOMP) gene and 16S rRNA gene sequences. Sequence analysis revealed significant homologies with C. pneumoniae compared to Chlamydia trachomatis and Chlamydia psittaci. High mean levels of CSF specific anti-C. pneumoniae antibodies and C. pneumoniae antibody specific index values significantly elevated were also found by enzyme-linked immunosorbent assay (ELISA) in these patients. The results suggest a hypothetical role of C. pneumoniae in the pathogenesis or progression of HADC.
authors.affiliation: Section of Infectious Diseases, Department of Clinical and Experimental Medicine, via Fossato di Mortara, 23, University of Ferrara, Italy. cnc@dns.unife.it
authors.forename: Carlo
authors.initials: C
authors.lastname: Contini
authors.forename: Enrico
authors.initials: E
authors.lastname: Fainardi
authors.forename: Silva
authors.initials: S
authors.lastname: Seraceni
authors.forename: Enrico
authors.initials: E
authors.lastname: Granieri
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Castellazzi
authors.forename: Rosario
authors.initials: R
authors.lastname: Cultrera
mesh_terms: D015526:AIDS Dementia Complex / Q000097:blood / Q000134:cerebrospinal fluid / Q000382:microbiology*; D000906:Antibodies / Q000134:cerebrospinal fluid; D000942:Antigens, Bacterial / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D002490:Central Nervous System / Q000276:immunology / Q000382:microbiology / Q000821:virology; D002690:Chlamydia Infections / Q000097:blood / Q000134:cerebrospinal fluid / Q000150:complications*; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity*; D004269:DNA, Bacterial / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000906:Antibodies; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D008565:Membrane Proteins; D012313:RNA