title: Involvement of herpes simplex type 2 in modulation of gene expression of human papillomavirus type 18.
abstract: Human papillomaviruses (HPVs) and herpes simplex virus type 2 (HSV-2) can establish latent or persistent infections in the host, and are involved in the aetiology of benign and/or malignant lesions of the urogenital tract. To investigate the putative interaction between these DNA viruses when a double infection occurs, we have studied the effect of HSV-2 infection in HeLa 229 cells containing 10-50 copies of HPV type 18 genomic DNA. Twenty hours post HSV-2 infection, the analysis of mRNA transcripts from E1, E2, E6 early and L1 late HPV18 genes was performed in HeLa cells by a semi-quantitative RT-PCR assay. A modulation of HPV18 E1 and E6 early genes was observed, resulting in a 9-fold and 3-fold increased transcription respectively.
authors.affiliation: Dept. Public Health Sciences, University of Rome, Italy.
authors.forename: S.
authors.initials: S
authors.lastname: Pisani
authors.forename: D.
authors.initials: D
authors.lastname: Fioriti
authors.forename: M. P.
authors.initials: MP
authors.lastname: Conte
authors.forename: F.
authors.initials: F
authors.lastname: Chiarini
authors.forename: L.
authors.initials: L
authors.lastname: Seganti
authors.forename: A. M.
authors.initials: AM
authors.lastname: Degener