Towards the identification of cassava root protein genes.
The protein population of cassava root layers was characterized by SDS-PAGE and bidimensional polyacrylamide gel electrophoresis. SDS-Page revealed the presence of a protein population in the molecular weight range between 94 and 20 kDa. The expression pattern of these proteins was well-defined within the different layers. Partial protein sequence analyses and preliminary results on the layer-specific expression pattern obtained with Northern analyses are presented.
De Souza
C R B
CR
Embrapa-Recursos Genéticos e Biotecnologia-CENARGEN, Parque Estação Biológica, PqEB-Final Av. W5 Norte, 70770-900 Brasília-DF, Brazil.
Carvalho
L J C B
LJ
De Almeida
E R P
ER
Gander
E S
ES
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D018744:DNA, Plant / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005784:Gene Amplification; D002365:Manihot / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D055315:Prolamins; D018749:RNA, Plant / Q000032:analysis; D017422:Sequence Analysis, DNA
D018744:DNA, Plant; D010940:Plant Proteins; D055315:Prolamins; D018749:RNA, Plant