An improved enrichment broth for isolation of Escherichia coli O157, with specific reference to starved cells, from radish sprouts.
An enrichment broth was developed for the efficient isolation of Escherichia coli O157 from radish sprouts. The broth was buffered peptone water containing 0.5% sodium thioglycolate (STG-BPW), which was designed to allow growth of E. coli O157 in starved and unstarved states. However, this medium suppressed the growth of non-carbohydrate-fermenting obligate aerobes whose colonial appearance on sorbitol MacConkey agar containing cefixime and tellurite (CT-SMAC) resembled that of E. coli O157. Both starved and unstarved cells of E. coli O157 experimentally inoculated into radish sprouts were successfully recovered with STG-BPW enrichment in all cases, most of which showed marked disappearance of E. coli O157-like colonies on CT-SMAC.
Sata
Shin
S
Kanagawa Prefectural Public Health Laboratory, Asahi-ku, Yokohama 241-0815, Japan.
Fujisawa
Tomohiko
T
Osawa
Ro
R
Iguchi
Atsushi
A
Yamai
Shiro
S
Shimada
Toshio
T
D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D001431:Bacteriological Techniques; D019607:Brassicaceae / Q000382:microbiology*; D002417:Cattle; D003470:Culture Media; D019453:Escherichia coli O157 / Q000254:growth & development* / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans
D003470:Culture Media