title: Effects of exercise on the secondary blood markers commonly used to suspect erythropoietin doping.
abstract: Numerous trials have reported that some haematological and biochemical parameters could be put together and be used to detect and fight recombinant erythropoietin doping. Unfortunately, none of the studies mentioned the necessity of taking pre-analytical precautions to avoid possible suspicious results coming from major plasma volume changes caused notably by dehydration. Therefore we studied the behaviour of the most common secondary blood markers before and after a strenuous physical activity to find out how reliable these parameters were. The soluble transferrin receptor and the haemoglobin concentrations as well as the haematocrit level increased significantly after effort, whereas the plasma EPO concentration and the reticulocyte count remained constant. On the other hand, if the values were corrected for haemoconcentration, the soluble transferrin receptor concentration remained stable.
authors.affiliation: Laboratoire Suisse d'Analyse du Dopage, Institut Universitaire de Médecine Légale, Lausanne, Switzerland. Neil.Robinson@inst.hospvd.ch
authors.forename: Neil
authors.initials: N
authors.lastname: Robinson
authors.forename: Martial
authors.initials: M
authors.lastname: Saugy
authors.forename: Patrice
authors.initials: P
authors.lastname: Mangin
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D004300:Doping in Sports; D004921:Erythropoietin / Q000097:blood*; D015444:Exercise / Q000502:physiology*; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D008297:Male; D010953:Plasma Volume / Q000502:physiology; D011990:Receptors, Transferrin / Q000097:blood; D017701:Reticulocyte Count; D015813:Substance Abuse Detection
chemical_list: D015415:Biomarkers; D006454:Hemoglobins; D011990:Receptors, Transferrin; D004921:Erythropoietin