title: Cre/lox: one more step in the taming of the genome.
abstract: Cre recombinase has become an important tool in the precise manipulation of the genome, and its adoption has led to the development of increasingly accurate mouse models for the understanding of gene function. Although much of current work exploits the alacrity and precision with which Cre catalyzes excisive DNA recombination, Cre also is adept at the insertion of heterologous DNA into the genome. The precision and efficiency with which Cre can target DNA to a predesignated locus in the genome promises to facilitate understanding of mutant genes and allelic variants in their natural chromosomal context.
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA. bls@stowers-institute.org
authors.forename: Brian
authors.initials: B
authors.lastname: Sauer
mesh_terms: D000818:Animals; D017101:Bacteriophage P1 / Q000235:genetics; D004247:DNA / Q000235:genetics; D018390:Gene Targeting; D016678:Genome; D019426:Integrases / Q000235:genetics*; D051379:Mice; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins; D004247:DNA; C045073:Cre recombinase; D019426:Integrases