title: The effect of extracellular calcium ion on gene expression of bone-related proteins in human pulp cells.
abstract: Calcium hydroxide is often used for induction of reparative dentin formation in endodontic treatment. However, little is known about the mechanism by which calcium hydroxide works. The calcium ion (Ca2+) is an important regulator of cell functions. In this study, we examined the effect of extracellular Ca2+ on gene expression of bone-related proteins in human cultured pulp cells in serum-free conditions. A Ca2+ level elevated by 0.7 mM induced an increase in mRNA expression of osteopontin and bone morphogenetic protein (BMP)-2. However, mRNA levels of BMP-4 and alkaline phosphatase decreased under the elevated Ca2+ culture condition. The same concentration of additional magnesium ions had little effect on expressions of the examined bone-related protein mRNAs. These findings suggest that Ca2+ in Ca(OH)2 specifically modulates osteopontin and BMP-2 levels during calcification in pulp.
authors.affiliation: Department of Periodontology and Endodontology, Hiroshima University Faculty of Dentistry, Japan.
authors.forename: Fahmida
authors.initials: F
authors.lastname: Rashid
authors.forename: Hideki
authors.initials: H
authors.lastname: Shiba
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Mizuno
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Mouri
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Fujita
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shinohara
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Ogawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawaguchi
authors.forename: Hidemi
authors.initials: H
authors.lastname: Kurihara
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000235:genetics; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis* / Q000235:genetics; D002118:Calcium / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003809:Dentin, Secondary / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D053495:Osteopontin; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000096:biosynthesis* / Q000235:genetics; D016212:Transforming Growth Factor beta
chemical_list: C525718:BMP2 protein, human; C525809:BMP4 protein, human; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D012333:RNA, Messenger; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D016212:Transforming Growth Factor beta; D053495:Osteopontin; D000469:Alkaline Phosphatase; D008274:Magnesium; D002126:Calcium Hydroxide; D002118:Calcium