Sequential waves of functionally related proteins are expressed when B cells prepare for antibody secretion.
Upon encounter with antigen, B lymphocytes differentiate into Ig-secreting plasma cells. This step involves a massive development of secretory organelles, most notably the endoplasmic reticulum. To analyze the relationship between organelle reshaping and Ig secretion, we performed a dynamic proteomics study of B lymphoma cells undergoing in vitro terminal differentiation. By clustering proteins according to temporal expression patterns, it appeared that B cells anticipate their secretory role in a multistep process. Metabolic capacity and secretory machinery expand first to accommodate the mass production of IgM that follows.
van Anken
Eelco
E
Department of Bio-organic Chemistry-1, Bijvoet Center, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.
Romijn
Edwin P
EP
Maggioni
Claudia
C
Mezghrani
Alexandre
A
Sitia
Roberto
R
Braakman
Ineke
I
Heck
Albert J R
AJ
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000276:immunology / Q000378:metabolism; D004734:Energy Metabolism; D015870:Gene Expression; D007075:Immunoglobulin M / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D010084:Oxidation-Reduction; D010950:Plasma Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D011506:Proteins / Q000235:genetics*; D040901:Proteomics
D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D011506:Proteins