Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides.
Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls. Recombinant XET from poplar has been purified from a Pichia pastoris expression system and crystallized. Two different crystal forms were obtained by vapour diffusion from potassium sodium tartrate and from an imidazole buffer using sodium acetate as a precipitant. Data were collected from these crystal forms to 3.5 and 2.1 A resolution, respectively. The first crystal form was found to belong to space group P3(1)21 or P3(2)21 (unit-cell parameters a = 98.6, b = 98.6, c = 98.5 A) and the second crystal form to space group P6(3) (unit-cell parameters a = 188.7, b = 188.7, c = 46.1 A).
Johansson
Patrik
P
Department of Cell and Molecular Biology, Uppsala University, Box 596, SE-75 124 Uppsala, Sweden. patrik@xray.bmc.uu.se
Denman
Stuart
S
Brumer
Harry
H
Kallas
Asa M
AM
Henriksson
Hongbin
H
Bergfors
Terese
T
Teeri
Tuula T
TT
Jones
T Alwyn
TA
D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D016695:Glycosyltransferases / Q000737:chemistry* / Q000302:isolation & purification; D007202:Indicators and Reagents; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D014197:Trees / Q000201:enzymology*
D007202:Indicators and Reagents; D011994:Recombinant Proteins; D016695:Glycosyltransferases; C073693:xyloglucan endotransglycosylase