title: Anti-parasitic activity of depudecin on Neospora caninum via the inhibition of histone deacetylase.
abstract: Neospora caninum is an apicomplexan parasite associated with abortion in cattle worldwide. Apicidin, histone deacetylase (HDAC) inhibitor, has shown a broad spectrum of anti-protozoal activity against apicomplexan parasites. Cultured vero cells infected with N. caninum tachyzoites were treated with 0.5 microg/ml of depudecin, another known natural product isolated from Altermaria brassiciicola with anti-histone deacetylase activity, to examine the efficacy of depudecin against intracellular multiplication of N. caninum tachyzoite, using 15 ng/ml of apicidin as the control. Depudecin significantly inhibited the intracellular multiplication of N. caninum at a level similar to that of apicidin without exerting any cytotoxicity on the host vero cells. Acid/urea/Triton gel electrophoresis analysis show anti-parasitic activity of depudecin appears to be due to the inhibition of protozoal histone deacetylase, which induces hyperacetylation of histones in N. caninum.
authors.affiliation: Department of Bioscience and Biotechnology, Institute of Bioscience, Sejong University, Seoul 143-747, South Korea.
authors.forename: Ho Jeong
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Minjung
authors.initials: M
authors.lastname: Kim
authors.forename: Jung-Keun
authors.initials: JK
authors.lastname: Lee
authors.forename: Woo-Suk
authors.initials: WS
authors.lastname: Hwang
authors.forename: Dae-Yong
authors.initials: DY
authors.lastname: Kim
mesh_terms: D000466:Alkadienes / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology* / Q000633:toxicity; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D004852:Epoxy Compounds / Q000494:pharmacology* / Q000633:toxicity; D005233:Fatty Alcohols / Q000494:pharmacology* / Q000633:toxicity; D006367:HeLa Cells; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D018688:Neospora / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D014709:Vero Cells
chemical_list: D000466:Alkadienes; D000981:Antiprotozoal Agents; D004852:Epoxy Compounds; D005233:Fatty Alcohols; D056572:Histone Deacetylase Inhibitors; D006657:Histones; C072251:depudecin; D006655:Histone Deacetylases