Fast and single solid phase fluorescence spectroscopic batch procedure for (acetyl) salicylic acid determination in drug formulations.
A solid phase fluorescence spectroscopic batch procedure for (acetyl) salicylic acid in drug formulations have been developed. The procedure is based on the sorption of salicylic acid (SA) on Sephadex DEAE A-25 anion exchanger gel (100 mg) by equilibration from an aqueous solution (10 or 25 ml) for 5 min; the equilibrated gel is transferred into an 1 mm quartz cell and the native fluorescence of SA sorbed on it is directly measured (lambda(ex)=297 nm; lambda(em)=405 nm). Good linearity was found in the 10-200 and 5-100 microg l(-1) ranges (for 10 and 25 ml sample volume, respectively) with R.S.D. (%) of 2.8 and 1.1. The procedure was successfully applied to the determination of acetyl salicylic acid (ASA) in drug formulations after alkaline hydrolysis to yield SA.
Ortega Algar
S
S
Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Paraje Las Lagunillas, E-23071 Jaén, Spain.
Ramos Martos
N
N
Molina Díaz
A
A
D000465:Algorithms; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D001241:Aspirin / Q000032:analysis*; D002110:Caffeine / Q000737:chemistry; D002214:Capsules; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D003061:Codeine / Q000737:chemistry; D004338:Drug Combinations; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D013050:Spectrometry, Fluorescence; D013607:Tablets
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002214:Capsules; D004338:Drug Combinations; D007202:Indicators and Reagents; D013607:Tablets; D002110:Caffeine; D001241:Aspirin; D003061:Codeine