title: Bone marrow transplantation abolishes inhibition of arteriogenesis in placenta growth factor (PlGF) -/- mice.
abstract: We studied the influence of placenta growth factor (PlGF) on arteriogenesis and tested the role of bone marrow (BM)-derived cells on PlGF-depleted vascular growth.
  
  
  METHODS
  Right femoral artery was occluded in wild type (+/+), PlGF k.o. (-/-) and (-/-) mice receiving BM from (+/+) mice (-/- BM). Blood supply in paws was assessed by laser-Doppler imaging (LDI) measurements before, immediately after, as well as 3, 7, 14, 21 and 28 days after surgery. Seven and 28 days after occlusion, hindlimbs were perfusion fixed and filled with contrast medium. Angiograms were evaluated, collateral arteries were analysed histologically and morphometrically.
  
  
  RESULTS
  Seven days after occlusion the blood flow and collateral growth in (-/-) were delayed in comparison to the (+/+) group. BM transplantation from +/+ donors prevented this delay. After 3 weeks the (-/-) mice reached values similar to the +/+ control.
  
  
  CONCLUSIONS
  PlGF is an important promoter of arteriogenesis. BM transplantation abolishes inhibition of arteriogenesis in PlGF -/- mice.
authors.affiliation: Department of Experimental Cardiology, Max-Planck-Institute, Benekestrasse 2, D-61231 Bad Nauheim, Germany. d.scholz@kerckhoff.mpg.de
authors.forename: Dimitri
authors.initials: D
authors.lastname: Scholz
authors.forename: Hanno
authors.initials: H
authors.lastname: Elsaesser
authors.forename: Annegret
authors.initials: A
authors.lastname: Sauer
authors.forename: Christian
authors.initials: C
authors.lastname: Friedrich
authors.forename: Aernout
authors.initials: A
authors.lastname: Luttun
authors.forename: Peter
authors.initials: P
authors.lastname: Carmeliet
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schaper
mesh_terms: D000792:Angiography; D000818:Animals; D001158:Arteries / Q000033:anatomy & histology / Q000502:physiology*; D016026:Bone Marrow Transplantation; D003097:Collateral Circulation; D005121:Extremities / Q000098:blood supply; D005263:Femoral Artery / Q000033:anatomy & histology / Q000000981:diagnostic imaging; D017078:Laser-Doppler Flowmetry; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009000:Monocytes / Q000737:chemistry; D000072483:Placenta Growth Factor; D011257:Pregnancy Proteins / Q000235:genetics / Q000502:physiology*; D012039:Regional Blood Flow
chemical_list: C000608037:Pgf protein, mouse; D011257:Pregnancy Proteins; D000072483:Placenta Growth Factor