Antimutagenic effect of Lentinula edodes (BERK.) Pegler mushroom and possible variation among lineages.
This study was performed to evaluate the efficiency of four different lineages (95/01, L1, 96/22 and JABK) of Lentinula edodes (BERK.) Pegler mushroom (shiitake) for inhibiting the N-ethyl-N-nitrosourea (ENU) clastogenicity in vivo. Male Swiss mice (10 animals/group) were treated during 15 consecutive days with dried mushroom added to basal diet under three different concentrations (1, 5 and 10%). At day 15, mice were intraperitoneally injected with ENU (50 mg/kg body weight) and sacrificed 24 h later for evaluation of micronucleated bone marrow polychromatic erythrocytes (MNPCE). Negative and positive controls (10 animals each), receiving basal diet and saline or ENU ip injection, respectively, were also evaluated. Results showed that pretreatments with diets containing the lineages 95/01, L1 and 96/22 reduce the frequencies of MNPCE induced by ENU. The absence of an antimutagenic activity for the lineage JABK might be related to intrinsic differences among the lineages such as biochemical composition. Taken together, our data show that the differences in protective activities of the mushrooms need to be clarified in further studies and the mechanisms for such activities need to be investigated.
Sugui
M M
MM
Faculdade de Medicina, UNESP, Botucatu, São Paulo, Brazil.
Alves de Lima
P L
PL
Delmanto
R D
RD
da Eira
A F
AF
Salvadori
D M F
DM
Ribeiro
L R
LR
D000818:Animals; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D001853:Bone Marrow / Q000187:drug effects; D004032:Diet; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects / Q000648:ultrastructure; D005038:Ethylnitrosourea / Q000633:toxicity; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity; D010641:Phenotype; D020942:Shiitake Mushrooms / Q000737:chemistry* / Q000235:genetics; D013045:Species Specificity
D016587:Antimutagenic Agents; D009153:Mutagens; D005038:Ethylnitrosourea