Dose-dependent biochemical changes in rat central nervous system after 12-week exposure to 1-bromopropane.
1-Bromopropane is used as a cleaning agent or adhesive solvent in the workplace. The present study investigated the long-term effects of exposure to 1-bromopropane on biochemical components in the central nervous system (CNS) of rats. Four groups, each of nine male Wistar rats, were exposed to 200, 400, or 800 ppm 1-bromopropane or fresh air only, 8h per day, 7 days a week for 12 weeks. We measured the levels of neuron-specific gamma-enolase, glia-specific beta-S100 protein, creatine kinase (CK) subunits B and M, heat shock protein Hsp27 (by enzyme immunoassay), enzymatic activity of CK and levels of glutathione (GSH), oxidized glutathione (GSSG) and sulfhydrul (SH) base in the cerebrum, cerebellum, brainstem and spinal cord. gamma-Enolase decreased dose-dependently in the cerebrum, which showed a decrease in wet weight, at 400 ppm or over, but no change was noted in beta-S100 protein in any brain region or spinal cord. Hsp27 decreased in the cerebellum, brainstem and spinal cord. Protein-bound SH base, non-protein SH base and total glutathione decreased in every brain region. CK activity decreased dose-dependently at 200 ppm or over, and the ratio of CK activity to CK-B concentration tended to decrease in all regions. The decrease in gamma-enolase in the cerebrum suggests the involvement of biochemical changes in neurons with decrease in the wet weight of the cerebrum. Glutathione depletion and changes in proteins containing SH base as a critical site might be the underlying neurotoxic mechanism of 1-bromopropane. The biochemical changes in the cerebrum indicate that long-term exposure to 1-bromopropane has effects on the CNS.
Wang
Hailan
H
Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya, Japan. wanghl@med.nagoya-u.ac.jp
Ichihara
Gaku
G
Ito
Hidenori
H
Kato
Kanefusa
K
Kitoh
Junzoh
J
Yamada
Tetsuya
T
Yu
Xiaozhong
X
Tsuboi
Seiji
S
Moriyama
Yoshinori
Y
Takeuchi
Yasuhiro
Y
D000818:Animals; D015415:Biomarkers; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects; D002490:Central Nervous System / Q000187:drug effects* / Q000378:metabolism*; D003402:Creatine Kinase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D006842:Hydrocarbons, Brominated / Q000633:toxicity*; D007118:Immunoassay; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010084:Oxidation-Reduction; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000633:toxicity*; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism
D015415:Biomarkers; D006842:Hydrocarbons, Brominated; D009419:Nerve Tissue Proteins; D012997:Solvents; D013438:Sulfhydryl Compounds; D003402:Creatine Kinase; D005978:Glutathione; C118559:1-bromopropane