Genotype identification of Orientia tsutsugamushi isolated from Nan Peng Lie Islands in China.
OBJECTIVE
To identify genotype of eight strains of Orientia tsutsugamushi (O. tsutsugamushi) isolated from Nan Peng Lie Islands in China and establish tsutsugamushi disease nature foci for this region.


METHODS
The nested polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used. Three primers were selected from the DNA sequence of the gene encoding type-specific 56-kDa protein of the Karp strain. The positive products were digested by Hine II and Pst I, meanwhile profiles specific to each strain were analyzed.


RESULTS
Three genotypes of O. tsutsugamushi including Karp, Kato and a new strain existed on Nan Peng Lie Islands.


CONCLUSIONS
Nan Peng Lie Islands is tsutsugamushi disease nature foci.
Peng
Guifu
G
Department of Epidemiology, Medical Institute, Guangzhou Command PLA, Guangzhou 510507, China.
Wang
Zhibing
Z
Wang
Shanshan
S
Huang
Jialiang
J
Jiang
Pulin
P
Zeng
Nianhua
N
Liu
Jinhua
J
Zhu
Shaofan
S
D000818:Animals; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D005838:Genotype; D051379:Mice; D012285:Orientia tsutsugamushi / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats
D004269:DNA, Bacterial