title: The bactericidal effects of Lactobacillus acidophilus, garcinol and Protykin compared to clarithromycin, on Helicobacter pylori.
abstract: Chronic infection with Helicobacter pylori causes peptic ulcers, gastric cancer and lymphoma. We evaluated the inhibitory effects of the probiotic Lactobacillus acidophilus DDS-1J, the antibiotic clarithromycin and the natural antioxidants garcinol and Protykin (containing 50% trans-resveratrol) on Helicobacter pylori strain ATCC 49503. The findings of this study indicate that Lactobacillus acidophilus DDS-1J exerts a growth inhibitory effect on H. pylori at a ratio of 1:1 or higher in vitro. In the case of clarithromycin, garcinol and resveratrol, the bactericidal effect is time and concentration dependent. Clarithromycin completely inhibited growth at > or = 62.5 microg/ml at 6 h and at > or = 31.5 microg/ml at 12 h. For garcinol the highest concentration needed for complete inhibition was 31.5 microg/ml at 6 h and 3.9 microg/ml after 12 h incubation. For resveratrol, significant inhibition was noted at 1000 microg/ml at 12 h only. The bactericidal effect of garcinol was reduced by the addition of resveratrol at all concentrations < or = 125 microg/ml at 6 and 12 h. We conclude from this study that Lactobacillus acidophilus DDS-1J inhibits H. pylori at 1:1 and higher ratios. Also, between the two antioxidants, garcinol is much more potent than resveratrol as a bactericidal agent against H. pylori, and that resveratrol may antagonize this effect. Finally, our study showed equivalent or better bactericidal activity of garcinol compared to clarithromycin against H. pylori at 6 and 12 h incubation, indicating a potential role for this antioxidant in treatment for H. pylori infection.
authors.affiliation: Department of Pediatrics, Creighton University Health Sciences Center, Omaha, NE 68178, USA. achatter@creighton.edu
authors.forename: Archana
authors.initials: A
authors.lastname: Chatterjee
authors.forename: Taharat
authors.initials: T
authors.lastname: Yasmin
authors.forename: Debasis
authors.initials: D
authors.lastname: Bagchi
authors.forename: Sidney J
authors.initials: SJ
authors.lastname: Stohs
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D017291:Clarithromycin / Q000494:pharmacology*; D018920:Coculture Techniques; D004305:Dose-Response Relationship, Drug; D016480:Helicobacter pylori / Q000187:drug effects* / Q000378:metabolism; D007779:Lactobacillus acidophilus / Q000378:metabolism*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013729:Terpenes / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000975:Antioxidants; D013267:Stilbenes; D013729:Terpenes; C477944:protykin; D017291:Clarithromycin; D000077185:Resveratrol; C054597:garcinol