Genomic structure, expression and characterization of a STAT5 homologue from pufferfish (Tetraodon fluviatilis).
The STAT5 (signal transducer and activator of transcription 5) gene was isolated and characterized from a round-spotted pufferfish genomic library. This gene is composed of 19 exons spanning 11 kb. The full-length cDNA of Tetraodon fluviatilis STAT5 (TfSTAT5) contains 2461 bp and encodes a protein of 785 amino acid residues. From the amino acid sequence comparison, TfSTAT5 is most similar to mouse STAT5a and STAT5b with an overall identity of 76% and 78%, respectively, and has < 35% identity with other mammalian STATs. The exon/intron junctions of the TfSTAT5 gene were almost identical to those of mouse STAT5a and STAT5b genes, indicating that these genes are highly conserved at the levels of amino acid sequence and genomic structure. To understand better the biochemical properties of TfSTAT5, a chimeric STAT5 was generated by fusion of the kinase-catalytic domain of carp Janus kinase 1 (JAK1) to the C-terminal end of TfSTAT5. The fusion protein was expressed and tyrosine-phosphorylated by its kinase domain. The fusion protein exhibits specific DNA-binding and transactivation potential toward an artificial fish promoter as well as authentic mammalian promoters such as the beta-casein promoter and cytokine inducible SH2 containing protein (CIS) promoter when expressed in both fish and mammalian cells. However, TfSTAT5 could not induce the transcription of beta-casein promoter via rat prolactin and Nb2 prolactin receptor. To our knowledge, this is the first report describing detailed biochemical characterization of a STAT protein from fish.
Sung
Shu-Chiun
SC
Graduate Institute of Life Science, National Defense Medical Center, Taipei, Taiwan.
Fan
Ting-Jia
TJ
Chou
Chih-Ming
CM
Leu
Jiann-Horng
JH
Hsu
Ya-Li
YL
Chen
Shui-Tsung
ST
Hsieh
Yueh-Chun
YC
Huang
Chang-Jen
CJ
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002347:Carps; D002364:Caseins / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D008894:Milk Proteins; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D050799:STAT5 Transcription Factor; D016415:Sequence Alignment; D017385:Sequence Homology; D023721:Tetraodontiformes / Q000235:genetics*; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism
D002364:Caseins; D004268:DNA-Binding Proteins; D008894:Milk Proteins; D011993:Recombinant Fusion Proteins; D050799:STAT5 Transcription Factor; C494094:Stat5a protein, rat; C494097:Stat5b protein, rat; D015534:Trans-Activators