Separation of peptides by pressurized capillary electrochromatography.
A pressurized electrochromatography (pCEC) instrument with gradient capability was used in this work for separation of peptides. Three separation modes, namely, pCEC, high-performance liquid chromatography and capillary electrophoresiscan be carried out with the instrument. In pCEC mode, the mobile phase is driven by both electroosmotic flow and pressurized flow, facilitating fine-tuning in selectivity of neutral and charged species. A continuous gradient elution can be carried out conveniently on this instrument, which demonstrates that it is more powerful than isocratic pCEC for separation of complicated samples. The effects of applied voltage, supplementary pressure and ion-pairing agents on separation of peptides in gradient pCEC were investigated. The effects of flow-rate of the pump and the volume of the mixer on resolution were also evaluated.
Zhang
Kai
K
State Key Laboratory of Elemento-Organic Chemistry, Nankai University, 94 Weijin Road, Tianjin 300071, China.
Jiang
Zhengjin
Z
Yao
Chuanyi
C
Zhang
Zhichao
Z
Wang
Qinsun
Q
Gao
Ruyu
R
Yan
Chao
C
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D010455:Peptides / Q000302:isolation & purification*; D011312:Pressure
D010455:Peptides