The effect of parachlorophenol and camphorated parachlorophenol on nitric oxide production by a murine macrophage cell line, RAW264.7.
The aim of this study was to determine the effect of parachlorophenol (PCP) and camphorated parachlorophenol (CMCP) on nitric oxide (NO) production by a murine macrophage cell line, RAW264.7. The cells were incubated on plastic disks with either PCP or CMCP. Plastic adherent and nonadherent cells were subsequently stimulated with recombinant mouse IFN-gamma or bacterial lipopolysaccharide (LPS). Nitric oxide (NO) levels detected from the culture supernatants were determined by the Griess reaction. The results showed that PCP and CMCP diluted at 10(-1) but not at 10(-3) suppressed NO production by both plastic adherent and nonadherent cells, suggesting that both phenolic compounds may suppress NO production by murine macrophages in a dose-dependent manner.
Barid
Izzata
I
Department of Oral Biology and Conservative Dentistry, University of Jember, Jember, East Java, Indonesia.
Sosroseno
Wihaskoro
W
D000704:Analysis of Variance; D000818:Animals; D002164:Camphor / Q000008:administration & dosage / Q000494:pharmacology*; D002448:Cell Adhesion; D002460:Cell Line; D002733:Chlorophenols / Q000008:administration & dosage / Q000494:pharmacology*; D004202:Disinfectants / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004926:Escherichia coli; D005029:Ethylenediamines; D016166:Free Radical Scavengers / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology; D016018:Least-Squares Analysis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D013223:Statistics as Topic; D013424:Sulfanilamides
D002733:Chlorophenols; D004202:Disinfectants; D004338:Drug Combinations; D005029:Ethylenediamines; D016166:Free Radical Scavengers; C095000:Griess reagent; D008070:Lipopolysaccharides; D013424:Sulfanilamides; D009569:Nitric Oxide; C029107:4-chlorophenol; D002164:Camphor; C029804:camphorated parachlorophenol; D007371:Interferon-gamma