Phenotypic variation of Plasmodium falciparum merozoite proteins directs receptor targeting for invasion of human erythrocytes.
The members of the phylum Apicomplexa parasitize a wide range of eukaryotic host cells. Plasmodium falciparum, responsible for the most virulent form of malaria, invades human erythrocytes using several specific and high affinity ligand-receptor interactions that define invasion pathways. We find that members of the P. falciparum reticulocyte-binding homolog protein family, PfRh2a and PfRh2b, are expressed variantly in different lines. Targeted gene disruption shows that PfRh2b mediates a novel invasion pathway and that it functions independently of other related proteins. Phenotypic variation of the PfRh protein family allows P. falciparum to exploit different patterns of receptors on the erythrocyte surface and thereby respond to polymorphisms in erythrocyte receptors and to evade the host immune system.
Duraisingh
Manoj T
MT
The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Plant Cell Biology Research Centre, School of Botany, University of Melbourne 3010, Australia.
Triglia
Tony
T
Ralph
Stuart A
SA
Rayner
Julian C
JC
Barnwell
John W
JW
McFadden
Geoffrey I
GI
Cowman
Alan F
AF
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D002918:Chymotrypsin / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology*; D004912:Erythrocytes / Q000469:parasitology*; D018390:Gene Targeting; D017125:Genes, Protozoan; D006801:Humans; D008954:Models, Biological; D009439:Neuraminidase / Q000378:metabolism; D010641:Phenotype; D010963:Plasmodium falciparum / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism
D000913:Antibodies, Protozoan; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; D009439:Neuraminidase; D002918:Chymotrypsin; D014357:Trypsin