title: Healing of unstented tubularized incised plate urethroplasty: an experimental study in a rabbit model.
abstract: OBJECTIVE
  To develop a rabbit model to study the temporal healing taking place after an unstented tubularized incised plate urethroplasty (TIPU).
  
  
  METHODS
  The study comprised 13 New Zealand white rabbits (3-4 kg); the ventral wall of the penile urethra was excised to create a hypospadias-like defect. A vertical incision was made in the dorsal urethral plate and the incised urethra tubularized. Two animals were killed at 2 days and two at 5 days after surgery, and the remainder killed at 2, 6 and 12 weeks (three each). A retrograde urethrogram was taken at autopsy. Serial sections of the penis were stained with haematoxylin and eosin, and Masson trichrome for microscopy.
  
  
  RESULTS
  There were no deaths related to the procedure and all animals voided spontaneously. Retrograde urethrograms showed no fistulae or stricture. Microscopic examination at 2 and 5 days showed partial coverage of the incision with regenerating urothelium. At 2 weeks there was full-thickness urothelium with a mild inflammatory reaction. At 6 and 12 weeks, remodelling of the peri-urethral connective tissue with minimal fibrosis completed the healing.
  
  
  CONCLUSIONS
  The mechanism of healing of the incised urethral plate involves normal urothelial regeneration into the depth of the incised defect, which explains the gain in urethral diameter after TIPU. Urine flow, during normal voiding, might be responsible for keeping the incised plate open during urothelial regeneration. A urethral stent was not necessary for normal healing in this model.
authors.affiliation: Division of Urology and Pathology, Hospital for Sick Children, University of Toronto, Ontario, Canada.
authors.forename: A T
authors.initials: AT
authors.lastname: Hafez
authors.forename: D
authors.initials: D
authors.lastname: Herz
authors.forename: D
authors.initials: D
authors.lastname: Bägli
authors.forename: C R
authors.initials: CR
authors.lastname: Smith
authors.forename: G
authors.initials: G
authors.lastname: McLorie
authors.forename: A E
authors.initials: AE
authors.lastname: Khoury
mesh_terms: D000818:Animals; D008297:Male; D010413:Penis / Q000601:surgery; D011817:Rabbits; D015607:Stents; D014521:Urethra / Q000601:surgery*; D014945:Wound Healing / Q000502:physiology*