Influence of diethyldithiocarbamate on the activity of ecto-ATPase in lymphocytes of rats: ex vivo studies.
Influence of diethyldithiocarbamate (DTC) on the activity of ecto-ATPase (plasma membrane-bound enzyme participating in a cascade of reactions leading to the formation of adenosine--a modulator of inflammation) was examined on the lymphocytes isolated from the spleen of rats with inflammation. DTC was administered at doses of 4 mg/kg and 290 mg/kg using two modes of administration. It has been observed that: a) an inflammation caused an increase in ecto-ATPase activity in both subpopulations of lymphocytes; in the case of B-lymphocytes, the maximum of activity occurred 48 h and in the case of T-lymophocytes, 72 h after the injection of carrageenin; b) a single injection of DTC at both doses, 24 h before or 24 h after carrageenin injection caused a decrease in ecto-ATPase activity in B-lymphocytes and its increase in T-lymphocytes throughout the whole measurement period, which was not observed when DTG was administered only after provocation of inflammation; c) administration of a high dose of DTC together with equimolar doses of disulfiram and CS2 led to a decrease in ecto-ATPase activity and 5 '-nucleotidase level in B-lymphocytes, which is bound to the former enzyme; d) in in vitro studies, both populations of lymphocytes isolated from the rats treated with a four-fold dose of DTC showed higher resistance of ecto-ATPase to inhibitors of the enzyme and antagonists of type P2 purinoceptors.
Purzyc
Leszek
L
Department of Medical Biochemistry, Wrocław Medical University, Chalubińskiego 10, PL 50-368 Wrocław, Poland. purzyc@idea.net.pl
Całkosińiski
Ireneusz
I
Polak-Jonkisz
Dorota
D
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D002351:Carrageenan; D004050:Ditiocarb / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007249:Inflammation / Q000139:chemically induced / Q000201:enzymology / Q000276:immunology; D051381:Rats; D011915:Rats, Inbred BUF; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D013997:Time Factors
D000276:Adjuvants, Immunologic; D002351:Carrageenan; D004050:Ditiocarb; D000251:Adenosine Triphosphatases; C027201:ectoATPase