Somatostatin suppresses ghrelin secretion from the rat stomach.
Ghrelin is an acylated peptide that stimulates food intake and the secretion of growth hormone. While ghrelin is predominantly synthesized in a subset of endocrine cells in the oxyntic gland of the human and rat stomach, the mechanism regulating ghrelin secretion remains unknown. Somatostatin, a peptide produced in the gastric oxyntic mucosa, is known to suppress secretion of several gastrointestinal peptides in a paracrine fashion. By double immunohistochemistry, we demonstrated that somatostatin-immunoreactive cells contact ghrelin-immunoreactive cells. A single intravenous injection of somatostatin reduced the systemic plasma concentration of ghrelin in rats. Continuous infusion of somatostatin into the gastric artery of the vascularly perfused rat stomach suppressed ghrelin secretion in both dose- and time-dependent manner. These findings indicate that ghrelin secretion from the stomach is regulated by gastric somatostatin.
Shimada
Mitsushi
M
Department of Internal Medicine, Oita Medical University, Oita 879-5593, Japan.
Date
Yukari
Y
Mondal
Muhtashan S
MS
Toshinai
Koji
K
Shimbara
Takuya
T
Fukunaga
Kyoko
K
Murakami
Noboru
N
Miyazato
Mikiya
M
Kangawa
Kenji
K
Yoshimatsu
Hironobu
H
Matsuo
Hisayuki
H
Nakazato
Masamitsu
M
D000818:Animals; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005753:Gastric Mucosa / Q000378:metabolism*; D054439:Ghrelin; D007150:Immunohistochemistry; D008297:Male; D015282:Octreotide / Q000494:pharmacology; D036361:Peptide Hormones / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D010477:Perfusion; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000494:pharmacology* / Q000502:physiology*; D013270:Stomach / Q000187:drug effects; D013997:Time Factors
D054439:Ghrelin; D036361:Peptide Hormones; D013004:Somatostatin; D015282:Octreotide