title: Evaluation of the cytotoxic and mutagenic potentiality of technetium-99m in Escherichi coli.
abstract: Since technetium-99m (99mTc) was introduced in medical research it has become one of the most employed radionuclides in nuclear medicine. 99mTc is ideal for routine use on the labeling of different radiopharmaceuticals due to its favorable characteristics. However, some biological effects have been described. These effects may be related to internal conversion electron and/or Auger electron emissions from 99mTc decay that present high linear energy transfer and can generate reactive oxygen species (ROS) in the medium. We evaluated in Escherichia coli K12S and Salmonella typhimurium TA102, both proficient in DNA repair, contribution of those decay emissions on the cytotoxicity induced by 99mTc, both either by generating lesions on DNA or by inducing alterations at membrane. We also studied the genotoxic and/or mutagenic potentiality of 99mTc, in Salmonella typhimurium, using the Ames test. The results showed that: i/ 99mTc is cytotoxic to the Escherichia coli K12S strains; ii/ this effect is related to the electrons (Auger and internal conversion) emissions, and iii/ the 99mTc is not mutagenic and/or genotoxic, when measured by Ames test.
authors.affiliation: Departamento de Biofisica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Av. 28 de Setembro, 87, CEP 20551-030, Rio de Janeiro, RJ, Brazil.
authors.forename: C R
authors.initials: CR
authors.lastname: Silva
authors.forename: J O
authors.initials: JO
authors.lastname: Valsa
authors.forename: A
authors.initials: A
authors.lastname: Caldeira-de-araújo
authors.forename: A
authors.initials: A
authors.lastname: Amaral
authors.forename: M
authors.initials: M
authors.lastname: Bernardo-Filho
mesh_terms: D004583:Electrons / Q000009:adverse effects; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D019275:Radiopharmaceuticals / Q000633:toxicity; D017382:Reactive Oxygen Species / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D013045:Species Specificity; D013667:Technetium / Q000633:toxicity*
chemical_list: D009153:Mutagens; D019275:Radiopharmaceuticals; D017382:Reactive Oxygen Species; D013667:Technetium