Construction and evaluation of internal control DNA for PCR amplification of Chlamydia trachomatis DNA from urine samples.
An internal control DNA (ICD) with the same primer binding sequences as the target Chlamydia trachomatis DNA was constructed and evaluated in a PCR assay with immunoenzymatic detection. One hundred urine specimens were tested, and 23 were found to contain inhibitors of the PCR, if not subjected to DNA extraction prior to amplification. Coamplification and detection of the ICD appeared to be a useful method for estimating the effects of inhibitors on C. trachomatis DNA amplification.
Betsou
Fotini
F
Biobanque de Picardie, 80000 Amiens, France. fay.betsou@biobanque-picaridie.com
Beaumont
Katy
K
Sueur
Jean Marie
JM
Orfila
Jeanne
J
D015373:Bacterial Typing Techniques; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis*; D005784:Gene Amplification; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011786:Quality Control; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D014556:Urine / Q000382:microbiology*
D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic