Enhanced pharmaceutical analysis by CE using dynamic surface coating system.
The poor repeatability of migration times in capillary electrophoresis (CE) within an injection sequence and between capillaries can be a difficulty when implementing CE for routine pharmaceutical analysis. The use of a dynamic surface coating has been shown to improve the routine performance of CE. The surface coating generates an appreciable electro-osmotic flow at low pH, which reduces analysis times for basic drugs compared to the low pH buffers typically used in CE. The repeatability of migration times and repeatability of migration times between capillaries was improved. Peak tailing for basic drugs was also reduced which improved peak shapes and peak area integration precision. It is concluded that the dynamic coating system is a positive advance in the routine implementation of CE into pharmaceutical analysis.
Altria
K D
KD
GlaxoSmithKline Research and Development, Pharmaceutical Development Europe, Park Road, Ware, Herts SG12 0DP, UK. kda8029@gsk.com
D002021:Buffers; D002138:Calibration; D004340:Drug Contamination; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009995:Osmosis; D004364:Pharmaceutical Preparations / Q000032:analysis; D015203:Reproducibility of Results
D002021:Buffers; D004364:Pharmaceutical Preparations