Structural analysis of conjugated linoleic acid produced by Lactobacillus plantarum, and factors affecting isomer production.
An isomer of the conjugated linoleic acid (CLA) produced from linoleic acid by Lactobacillus plantarum was identified as cis-9,trans-11-octadecadienoic acid by proton nuclear magnetic resonance spectroscopy. Together with earlier results, we concluded that the bacterium produces two CLA isomers, cis-9,trans-11- and trans-9,trans-11-octadecadienoic acid from linoleic acid. The addition of L-serine, glucose, AgNO3, or NaCl to the reaction mixture reduced production of the latter.
Kishino
Shigenobu
S
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Ogawa
Jun
J
Ando
Akinori
A
Iwashita
Takashi
T
Fujita
Tsuyoshi
T
Kawashima
Hiroshi
H
Shimizu
Sakayu
S
D003470:Culture Media; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005227:Fatty Acids / Q000737:chemistry; D005947:Glucose / Q000494:pharmacology; D007536:Isomerism; D007778:Lactobacillus / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D019787:Linoleic Acid / Q000096:biosynthesis / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D012694:Serine / Q000494:pharmacology; D012835:Silver Nitrate / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology
D003470:Culture Media; D004791:Enzyme Inhibitors; D005227:Fatty Acids; D012965:Sodium Chloride; D012694:Serine; D012835:Silver Nitrate; D019787:Linoleic Acid; D005947:Glucose