Crystallization and preliminary X-ray study of human dipeptidyl peptidase IV (DPPIV).
Human DPPIV has been expressed in the baculovirus system and purified and crystallized using the hanging-drop method. A crystal was obtained from 180 mM Gly-NaOH buffer pH 9.5 containing 18% PEG 4000 and 180 mM sodium acetate. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 118.04, b = 125.92, c = 136.84 A, and diffracts beyond 2.6 A resolution. There are two molecules per asymmetric unit, indicating a solvent content of 57.6%.
Hiramatsu
Hajime
H
Discovery Research Laboratory, Tanabe Seiyaku Co. Ltd, 3-16-89 Kashima, Yodogawa-ku, Osaka 532-8505, Japan.
Kyono
Kiyoshi
K
Shima
Hideaki
H
Fukushima
Chiaki
C
Sugiyama
Shigeru
S
Inaka
Koji
K
Yamamoto
Atsushi
A
Shimizu
Ryo
R
D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D018076:DNA, Complementary / Q000096:biosynthesis; D018819:Dipeptidyl Peptidase 4 / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D018819:Dipeptidyl Peptidase 4