Synthesis and secretion of monocyte chemotactic protein-1 stimulated by the high affinity receptor for IgE.
In prior studies aggregation of the high affinity receptors for IgE, Fc epsilon RI, on a rat mast cell line, RBL-2H3, stimulated transcription of the gene for monocyte chemotactic protein-1 (MCP-1) and secretion of the protein. Unexpectedly, those delayed events appeared much less constrained by kinetic proofreading than had been documented for other receptor-initiated responses. The results of the present experiments are consistent with the proposal that the biosynthesis and secretion of MCP-1 result from a soluble messenger formed in the reaction cascades initiated by the receptor, and that Ca(2+) could serve as that messenger. Interestingly, whereas receptor-mediated signals were required for transcription of the gene for MCP-1 and secretion of the chemokine, such signals were not required for the intervening step of translation of its mRNA.
Eglite
Santa
S
Section on Chemical Immunology, Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Morin
Juan M
JM
Metzger
Henry
H
D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects / Q000276:immunology; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D051966:Phospholipase C gamma; D010766:Phosphorylation; D014176:Protein Biosynthesis / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011940:Receptor Aggregation / Q000276:immunology; D017455:Receptors, IgE / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic / Q000187:drug effects / Q000276:immunology; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000502:physiology; D014443:Tyrosine / Q000378:metabolism
D018932:Chemokine CCL2; D008024:Ligands; D012333:RNA, Messenger; D017455:Receptors, IgE; D003609:Dactinomycin; D014443:Tyrosine; D010738:Type C Phospholipases; D051966:Phospholipase C gamma; D002118:Calcium