Nicotiana benthamiana gp91phox homologs NbrbohA and NbrbohB participate in H2O2 accumulation and resistance to Phytophthora infestans.
Active oxygen species (AOS) are responsible for triggering defense responses in plants. Respiratory burst oxidase homologs (rboh genes) have been implicated in AOS generation. We have isolated two rboh cDNAs, NbrbohA and NbrbohB, from Nicotiana benthamiana leaves. NbrbohA was expressed constitutively at a low level and the transcripts were increased after mechanical stress of control leaf infiltration, whereas NbrbohB was induced specifically by the protein elicitor INF1 from the potato pathogen Phytophthora infestans. We examined the function of the Nbrboh genes in AOS generation and in the hypersensitive response (HR) using virus-induced gene silencing (VIGS). VIGS indicated that both genes are required for H2O2 accumulation and for resistance to Phytophthora. VIGS of Nbrboh genes also led to a reduction and delay of HR cell death caused by INF1. We further demonstrate that the induction of HR-like cell death by overexpression of a constitutively active mutant of a mitogen-activated protein kinase kinase, MEK(DD), is compromised by VIGS of NBRBOHB: We found that MEK(DD) induced NbrbohB but not NBRBOHA: This work provides genetic evidence for the involvement of a mitogen-activated protein kinase cascade in the regulation of rboh genes.
Yoshioka
Hirofumi
H
Plant Pathology Laboratory, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601 Japan. hyoshiok@agr.nagoya-u.ac.jp
Numata
Noriko
N
Nakajima
Kazumi
K
Katou
Shinpei
S
Kawakita
Kazuhito
K
Rowland
Owen
O
Jones
Jonathan D G
JD
Doke
Noriyuki
N
D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007113:Immunity, Innate / Q000187:drug effects / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009154:Mutation; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; D054883:Oxylipins; D010802:Phylogeny; D010838:Phytophthora / Q000254:growth & development*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology
D000085:Acetates; D003517:Cyclopentanes; D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D054883:Oxylipins; D010940:Plant Proteins; D017382:Reactive Oxygen Species; C072239:methyl jasmonate; D006861:Hydrogen Peroxide; D009247:NADH, NADPH Oxidoreductases; C056558:CYBB protein, human; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; D020929:Mitogen-Activated Protein Kinase Kinases; D020156:Salicylic Acid