title: Mouse round spermatids developed in vitro from preexisting spermatocytes can produce normal offspring by nuclear injection into in vivo-developed mature oocytes.
abstract: It has been shown that mature oocytes injected with nuclei from round spermatids collected from mouse testis can generate normal offspring and that round spermatids can develop in vitro. An undetermined issue is whether spermatids developed in vitro are capable of generating fertile offspring by nuclear injection into oocytes. Herein, we report the production of normal and fertile offspring by nuclear injection using haploid spermatid donors derived from mouse primary spermatocyte precursors cocultured with Sertoli cells. Cocultured spermatogonia and spermatocytes were characterized by their nuclear immunoreactive patterns determined by an antibody to phosphorylated histone H2AX (gamma-H2AX), a marker for DNA double-strand breaks. Cocultured round spermatid progenies display more than one motile flagellum, whose axonemes were recognized by antitubulin immunostaining. Flagellar wavelike movement and flagellar-driven propulsion of round spermatids developed in vitro were documented by videomicroscopy (http://www.sci.ccny.cuny.edu/ approximately kier). We also show that breeding of male and female mouse offspring generated by spermatid nuclear injection produced fertile offspring. In addition to their capacity to produce fertile offspring, cocultured, flagellated round spermatids can facilitate the analysis of the mechanisms of centriolar polarity, duplication, assembly, and flagellar growth, including the intraflagellar transport of cargo proteins.
authors.affiliation: The Institute for Biogenesis Research, Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, Hawaii 96822, USA.
authors.forename: Joel
authors.initials: J
authors.lastname: Marh
authors.forename: Laura L
authors.initials: LL
authors.lastname: Tres
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Yanagimachi
authors.forename: Abraham L
authors.initials: AL
authors.lastname: Kierszenbaum
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D018920:Coculture Techniques; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000254:growth & development*; D011247:Pregnancy; D012708:Sertoli Cells / Q000166:cytology; D013087:Spermatids / Q000166:cytology* / Q000648:ultrastructure; D013090:Spermatocytes / Q000166:cytology*