title: Identification of Histoplasma capsulatum from culture extracts by real-time PCR.
abstract: We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.
authors.affiliation: Division of Infectious Diseases. Section of Clinical Microbiology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
authors.forename: Jose
authors.initials: J
authors.lastname: Martagon-Villamil
authors.forename: Nabin
authors.initials: N
authors.lastname: Shrestha
authors.forename: Mary
authors.initials: M
authors.lastname: Sholtis
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Isada
authors.forename: Gerri S
authors.initials: GS
authors.lastname: Hall
authors.forename: Terry
authors.initials: T
authors.lastname: Bryne
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Lodge
authors.forename: L Barth
authors.initials: LB
authors.lastname: Reller
authors.forename: Gary W
authors.initials: GW
authors.lastname: Procop
mesh_terms: D004271:DNA, Fungal / Q000032:analysis; D006658:Histoplasma / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008828:Microbiological Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004271:DNA, Fungal