title: [Growth and osteogenesis characteristics of cultured rat marrow stromal cells under bone induction condition].
abstract: OBJECTIVE
  To investigate the growth and osteogenesis characteristics of cultured rat marrow stromal cells(rMSCs) under bone induction condition.
  
  
  METHODS
  rMSCs were isolated from adult rat using density gradient separation method. The rMSCs attachment formed soon after seeding and grew into colonies with the appearance of fibroblastic cells. The osteogenic induction compound of Dex(10(-8) mol/L), beta-GP(10 mmol/L) and AA(50 micrograms/ml) was added to different passaged rMSCs and the proliferation and osteogenic differentiation of them was observed.
  
  
  RESULTS
  The induction compound had strong effect on promoting proliferation, especially on that of further passaged rMSCs, and its stimulation effect on osteogenesis was also well proved with the expression of alkaline phosphatase (ALP) rising after one-week induction. Further subculturing from P1 to P6 led to the increase of proliferation activity of rMSCs. After three-week induction, mineral deposits appeared in the culture.
  
  
  CONCLUSIONS
  The lower expression of ALP in P1 rMSCs and the increase of it in further passages or under induction condition suggest the rMSCs in our culture system are mainly undifferentiated osteoprogenitors.
authors.affiliation: Biomedical Engineering Research Unit, WCUMS, Chengdu 610041, China.
authors.forename: J
authors.initials: J
authors.lastname: Han
authors.forename: R
authors.initials: R
authors.lastname: Chen
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Deng
authors.forename: F
authors.initials: F
authors.lastname: Tang
authors.forename: W
authors.initials: W
authors.lastname: Tian
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001846:Bone Development; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D005994:Glycerophosphates / Q000494:pharmacology; D010012:Osteogenesis; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology* / Q000201:enzymology
chemical_list: D005994:Glycerophosphates; D003907:Dexamethasone; D000469:Alkaline Phosphatase; D001205:Ascorbic Acid; C031463:beta-glycerophosphoric acid