title: Targeted recycling of PECAM from endothelial surface-connected compartments during diapedesis.
abstract: Leukocytes enter sites of inflammation by squeezing through the borders between endothelial cells that line postcapillary venules at that site. This rapid process, called transendothelial migration (TEM) or diapedesis, is completed within 90 s after a leukocyte arrests on the endothelial surface. In this time, the leukocyte moves in ameboid fashion across the endothelial borders, which remain tightly apposed to it during transit. It is not known how the endothelial cell changes its borders rapidly and reversibly to accommodate the migrating leukocyte. Here we show that there is a membrane network just below the plasmalemma at the cell borders that is connected at intervals to the junctional surface. PECAM-1, an integral membrane protein with an essential role in TEM, is found in this compartment and constitutively recycles evenly along endothelial cell borders. During TEM, however, recycling PECAM is targeted to segments of the junction across which monocytes are in the act of migration. In addition, blockade of TEM with antibodies against PECAM specifically blocks the recruitment of this membrane to the zones of leukocyte migration, without affecting the constitutive membrane trafficking.
authors.affiliation: Department of Pathology and Laboratory Medicine, Weill Medical College, 1300 York Avenue, New York, New York 10021, USA.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Mamdouh
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.forename: Lynda M
authors.initials: LM
authors.lastname: Pierini
authors.forename: Frederick R
authors.initials: FR
authors.lastname: Maxfield
authors.forename: William A
authors.initials: WA
authors.lastname: Muller
mesh_terms: D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D005434:Flow Cytometry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D010539:Permeability; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism* / Q000648:ultrastructure; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1