Activity of glutathione S-transferase in Spodoptera exigua larvae exposed to cadmium and zinc in two subsequent generations.
The aim of our study was to establish changes in activity of important in detoxification enzyme-glutathione S-transferase (GST): in alimentary tract, fat body and Malpighian tubules of Spodoptera exigua larvae being under cadmium and zinc exposure through the first as well as the second generation. There was registered enhancement of the enzyme activity in the fat body and the Malpighian tubules caused by zinc as well as its decrease in the Malpighian tubules under cadmium action. Amounts of metals in the alimentary tract were either several times higher than in the diet ingested by larvae or than in the fat body. Metal concentration in the fat body correlated with the level of the enzyme activity (positive correlation for zinc and negative for cadmium). The effect of metal action differentiated dependently on time exposition.
Kafel
Alina
A
Department of Animal Physiology and Ecotoxicology, University of Silesia, 9 Bankowa Street, 40-007, Katowice, Poland. akafel@us.edu.pl
Bednarska
Katarzyna
K
Augustyniak
Maria
M
Witas
Ilona
I
Szuliñska
Elzbieta
E
D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D002104:Cadmium / Q000009:adverse effects* / Q000493:pharmacokinetics; D004064:Digestive System / Q000737:chemistry; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000009:adverse effects*; D005982:Glutathione Transferase / Q000494:pharmacology*; D007814:Larva; D018411:Spodoptera / Q000201:enzymology* / Q000235:genetics; D014018:Tissue Distribution; D015032:Zinc / Q000009:adverse effects* / Q000493:pharmacokinetics
D004785:Environmental Pollutants; D002104:Cadmium; D005982:Glutathione Transferase; D015032:Zinc