Prime-boost vaccination with plasmid DNA encoding caprine-arthritis encephalitis lentivirus env and viral SU suppresses challenge virus and development of arthritis.
This study evaluated the efficacy of prime-boost vaccination for immune control of caprine arthritis-encephalitis virus (CAEV), a macrophage tropic lentivirus that causes progressive arthritis in the natural host. Vaccination of Saanen goats with pUC-based plasmid DNA expressing CAEV env induces T helper type 1 (Th1) biased immune responses to vector-encoded surface envelope (SU), and the plasmid-primed Th1 response is expanded following boost with purified SU in Freund's incomplete adjuvant (SU-FIA) (J. C. Beyer et al., 2001, Vaccine 19, 1643-1651). Four goats vaccinated with env expression plasmids and boosted with SU-FIA were challenged intravenously with 1 x 10(4) TCID(50) of CAEV at 428 days after SU-FIA boost and evaluated by immunological, virological, and disease criteria. Controls included two goats primed with pUC18 and eight unvaccinated goats. Goats receiving prime-boost vaccination with CAEV env plasmids and SU-FIA became infected but suppressed postchallenge virus replication, provirus loads in lymph node, and development of arthritis for at least 84 weeks.
Cheevers
W P
WP
Department of Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA. cheevers@vetmed.wsu.edu
Snekvik
K R
KR
Trujillo
J D
JD
Kumpula-McWhirter
N M
NM
Pretty On Top
K J
KJ
Knowles
D P
DP
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001168:Arthritis / Q000517:prevention & control* / Q000821:virology; D016088:Arthritis-Encephalitis Virus, Caprine / Q000276:immunology* / Q000472:pathogenicity; D015686:Gene Products, env / Q000235:genetics / Q000276:immunology*; D006023:Glycoproteins; D015511:Goat Diseases / Q000517:prevention & control / Q000821:virology; D006041:Goats; D007117:Immunization, Secondary; D016180:Lentivirus Infections / Q000517:prevention & control* / Q000821:virology; D008198:Lymph Nodes / Q000821:virology; D008565:Membrane Proteins; D010957:Plasmids / Q000235:genetics; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology; D014764:Viral Proteins; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000914:Antibodies, Viral; C412317:ENV protein, Caprine arthritis encephalitis virus; D015686:Gene Products, env; D006023:Glycoproteins; D008565:Membrane Proteins; D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines