Simultaneous separation of fifteen approved protease and reverse transcriptase inhibitors for human immunodeficiency virus therapy by capillary electrophoresis.
In the present investigation, a novel approach towards a complete separation of all 15 protease and reverse transcriptase inhibitors which are currently approved for use in highly active antiretroviral therapy in a single analytical run is presented. The developed method employs an acidic background electrolyte with sodium polyanethol sulfonate (SPAS) as polyanionic electroosmotic flow (EOF) modifier to establish a strong cathodic EOF, sodium dodecyl sulfate (SDS) as pseudostationary selector, and acetonitrile and ethanol as organic modifiers. Separation of the analytes is based on two different mechanisms. The more basic analytes are protonated at the prevailing pH conditions and thus migrate in front of the cathodic EOF, whereas the less basic and neutral analytes interact with the SDS and are retained after the EOF. By optimizing electrolyte pH, the amount of solvents and SDS concentrations in the background electrolyte it is possible to completely separate all compounds of interest.
Tuan
Nguyen Duc
ND
Institute of Analytical Chemistry and Radiochemistry, Innsbruck, Autria.
Gutleben
Wolfgang
W
Scherer
Karin
K
Stoiber
Heribert
H
Falkensammer
Barbara
B
Dierich
Manfred P
MP
Zemann
Andreas
A
D019075:Electrophoresis, Capillary / Q000379:methods*; D015658:HIV Infections / Q000188:drug therapy; D017320:HIV Protease Inhibitors / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D018894:Reverse Transcriptase Inhibitors / Q000097:blood / Q000302:isolation & purification*
D017320:HIV Protease Inhibitors; D018894:Reverse Transcriptase Inhibitors