title: Differentially expressed genes associated with CIS-diamminedichloroplatinum (II) resistance in head and neck cancer using differential display and CDNA microarray.
abstract: BACKGROUND
  The mechanism by which cancer cells become resistant to cis-Diamminedichloroplatinum (II) (cDDP) is not completely understood. To investigate the molecular markers involved in the cDDP resistance, we compared the gene expression profiles between a head and neck squamous cell carcinoma (HNSCC) line sensitive to cDDP and its cDDP-resistant variant.
  
  
  METHODS
  Both a fluorescent differential display and a cDNA microarray analysis were applied to distinguish the gene profiles between KB, a human HNSCC line, and its cDDP-resistant variant (KB/cDDP). These results were confirmed by Northern blot analysis.
  
  
  RESULTS
  One up-regulated gene, glycoprotein hormone alpha-subunit, and two down-regulated genes coding membrane proteins, human folate receptor and tumor-associated antigen L6, were identified in KB/cDDP cells.
  
  
  CONCLUSIONS
  Our findings suggest that development of the cDDP-resistant phenotype is accompanied by alternations of gene expression including a glycoprotein hormone and membrane proteins. These gene products could be new molecular markers for resistance to cDDP.
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, Hokkaido University, Graduate School of Medicine, Kita 15, Nishi 7, Kita-ku, Sapporo 060-8638, Japan.
authors.forename: Eisaku
authors.initials: E
authors.lastname: Higuchi
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Oridate
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Furuta
authors.forename: Seigo
authors.initials: S
authors.lastname: Suzuki
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Hatakeyama
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sawa
authors.forename: Kousaku
authors.initials: K
authors.lastname: Sunayashiki-Kusuzaki
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Yamazaki
authors.forename: Yukio
authors.initials: Y
authors.lastname: Inuyama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fukuda
mesh_terms: D015152:Blotting, Northern; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000235:genetics*; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D018076:DNA, Complementary / Q000032:analysis*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D005819:Genetic Markers / Q000235:genetics; D006258:Head and Neck Neoplasms / Q000188:drug therapy / Q000235:genetics*; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010597:Pharmacogenetics; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured / Q000187:drug effects; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D005819:Genetic Markers; D008562:Membrane Glycoproteins; D002945:Cisplatin