title: Double immunolabeling by the RBM and the PLAP markers for identifying intratubular (in situ) germ cell neoplasia of the testis.
abstract: Identification of intratubular germ cell neoplasia (carcinoma in situ, CIS) of the testis is a diagnostic challenge, and markers are sorely needed to assist in accurately identifying the lesion. RNA-binding motif (RBM) protein, encoded by the Y chromosome, is expressed exclusively and consistently in differentiated male germ cells, while it is absent in neoplastic germ cells. Another immunohistochemical marker, placental alkaline phosphatase (PLAP), is commonly used for the detection of undifferentiated germ cells. The current study demonstrates that simultaneous use of the immunohistochemical markers, RBM and PLAP, by double immunolabeling enhances the accuracy of diagnosing CIS, a preinvasive testicular neoplasm.
authors.affiliation: Institute of Pathology, Lis Maternity Hospital, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
authors.forename: Letizia
authors.initials: L
authors.lastname: Schreiber
authors.forename: Beatriz
authors.initials: B
authors.lastname: Lifschitz-Mercer
authors.forename: Gedalia
authors.initials: G
authors.lastname: Paz
authors.forename: Haim
authors.initials: H
authors.lastname: Yavetz
authors.forename: David J
authors.initials: DJ
authors.lastname: Elliott
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kula
authors.forename: Jolanta
authors.initials: J
authors.lastname: Slowikowska-Hilczer
authors.forename: Batia Bar-Shira
authors.initials: BB
authors.lastname: Maymon
mesh_terms: D000469:Alkaline Phosphatase; D014408:Biomarkers, Tumor / Q000032:analysis; D002278:Carcinoma in Situ / Q000737:chemistry* / Q000473:pathology; D002675:Child, Preschool; D019084:Fluorescent Antibody Technique, Indirect; D058851:GPI-Linked Proteins; D018237:Germinoma / Q000737:chemistry* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D016601:RNA-Binding Proteins / Q000032:analysis*; D013736:Testicular Neoplasms / Q000737:chemistry* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D058851:GPI-Linked Proteins; D007527:Isoenzymes; D016601:RNA-Binding Proteins; D000469:Alkaline Phosphatase; C019839:alkaline phosphatase, placental