title: Lyme disease spirochete, Borrelia burgdorferi endemic at epicenter in Rondeau Provincial Park, Ontario.
abstract: The Lyme disease spirochete, Borrelia burgdorferi Johnson, Schmidt, Hyde, Steigerwalt, and Brenner was discovered in blacklegged ticks, Ixodes scapularis Say at Rondeau Provincial Park, Ontario, Canada During this 2-yr study, spirochetes were found in B. burgdorferi-positive I. scapularis larvae attached to B. burgdorferi-infected white-footed mice, Peromyscus leucopus Rafinesque. Isolates of B. burgdorferi were cultured from blacklegged tick adults, and confirmed positive with polymerase chain reaction by targeting OspA and rrf (5S)-rrl (23S) genes. These findings show an endemic area for B. burgdorferi within an established population of L. scapularis at Rondeau Provincial Park.
authors.affiliation: Laboratory Services, British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada V5Z 4R4. mmorshed@interchange.ubc.ca
authors.forename: Muhammad G
authors.initials: MG
authors.lastname: Morshed
authors.forename: John D
authors.initials: JD
authors.lastname: Scott
authors.forename: Keerthi
authors.initials: K
authors.lastname: Fernando
authors.forename: Robert B
authors.initials: RB
authors.lastname: Mann
authors.forename: Lance A
authors.initials: LA
authors.lastname: Durden
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001428:Bacterial Vaccines; D001483:Base Sequence; D025065:Borrelia burgdorferi / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D017931:DNA Primers; D007303:Insect Vectors; D018884:Ixodes; D008074:Lipoproteins; D008193:Lyme Disease / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D051379:Mice; D009864:Ontario / Q000453:epidemiology; D016133:Polymerase Chain Reaction
chemical_list: D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D017931:DNA Primers; D008074:Lipoproteins; C072045:OspA protein