[Effects of selenium dioxide on regulatory regions P250 of c-fos gene].
OBJECTIVE
This study was designed to investigate the impact of selenium dioxide (SeO2) on regulatory regions P250 of c-fos gene and to seek possible regulation mechanism.


METHODS
HeLa cells were transfected with plasmids containing upstream regulating regions of c-fos chloramphenicol acetyl-transferase (CAT). The cells were cultured in various concentration of selenium dioxide. CAT expression in transfected cells was observed.


RESULTS
After transfected HeLa cells were exposed to selenium dioxide, CAT expression showed obvious increase, especially in 10 micromol/L and 30 micromol/L selenium dioxide group (P< 0.05).


CONCLUSIONS
Trough affecting regulatory regions P250 of c-fos gene, Selenium dioxide plays biological effect of regulating tumor cells. Selenium dioxide possibly has anti-tumor effects.
Yu
Hai-Jian
HJ
Department of Respiratory Diseases, Union Hospital, Peking Union Medical University, Chinese Academy of Medical Sciences, Beijing, 100730, PR China. yuhaijian@vip.sina.com
Cao
Xiao-Zhe
XZ
Li
Long-Yun
LY
Zhao
Mei-Lan
ML
D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000187:drug effects*; D018036:Selenium Compounds / Q000494:pharmacology*; D064588:Selenium Oxides
D016760:Proto-Oncogene Proteins c-fos; D018036:Selenium Compounds; D064588:Selenium Oxides; D015500:Chloramphenicol O-Acetyltransferase