title: Allelic variation in srtAs of Streptococcus suis strains.
abstract: Streptococcus suis NCTC10234 possesses five srtA homologs: srtA encodes sortase, which anchors surface proteins with an LPXTG motif to the cell wall, while the functions of the other four homologs (the srtBCD cluster and srtE) remain unknown. The genetic organization of the srtA region was found to be conserved in the 59 S. suis strains examined in this study. Although the srtAs in three of these strains showed strong sequence divergence, their functions were verified to be overlapping by genetic complementation, indicating the functional conservation of srtAs during the evolution of these strains. These results indicate the importance of an srtA-mediated cell wall sorting system for displaying proteins on the surface of S. suis.
authors.affiliation: Molecular Bacteriology Section, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, 305-0856, Ibaraki, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Osaki
authors.forename: Daisuke
authors.initials: D
authors.lastname: Takamatsu
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shimoji
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Sekizaki
mesh_terms: D000483:Alleles; D019881:Aminoacyltransferases / Q000032:analysis / Q000235:genetics*; D001426:Bacterial Proteins; D001483:Base Sequence; D003470:Culture Media; D003546:Cysteine Endopeptidases; D014644:Genetic Variation; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D017011:Streptococcus suis / Q000145:classification / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases