title: Substrate inhibition of L-cysteine alpha,beta-elimination reaction catalyzed by L-cystathionine gamma-lyase of Saccharomyces cerevisiae.
abstract: The alpha,beta-elimination of L-cysteine catalyzed by Saccharomyces cerevisiae L-cystathionine gamma-lyase (EC 4.4.1.1) was inhibited by the substrate. The absorption spectrum of the holoenzyme in the presence of L-cysteine showed that the substrate inhibition observed in this reaction was due mainly to removal of the cofactor.
authors.affiliation: Department of Biotechnology, Faculty of Agriculture, Gifu University, Gifu 501-1193, Japan. yamagata@cc.gifu-u.ac.jp
authors.forename: Shuzo
authors.initials: S
authors.lastname: Yamagata
authors.forename: Motomi
authors.initials: M
authors.lastname: Isaji
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yamane
authors.forename: Tomonori
authors.initials: T
authors.lastname: Iwama
mesh_terms: D003542:Cystathionine gamma-Lyase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D003545:Cysteine / Q000378:metabolism* / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D003542:Cystathionine gamma-Lyase; D003545:Cysteine