title: Genetic variability of Fusarium wilt pathogen isolates of chickpea (Cicer arietinum L.) assessed by molecular markers.
abstract: Genetic variability among 43 isolates of Fusarium oxysporum f.sp. ciceri, the chickpea wilt pathogen, collected from nine states of India including the four well-characterized races of the pathogen were assessed using the molecular markers, RAPDs and AFLP. Principal coordinate analysis of the similarity index data generated from the molecular marker studies mostly gave three different clusters: Of these two clusters represented race-1 and race-2, and the third cluster consisted of race-3 and race-4 pathogen isolates. In RAPDs a fourth cluster was seen which did not go with any of the four races of the pathogen. The molecular markers established the distinctness of race-1 and race-2 pathogen isolates and the close similarity of pathogen isolates of race-3 with that of race-4. AFLP was found to be more informative as it differentiated more number of the pathogen isolates with the known races with minimum of outliers. The high levels of DNA polymorphism observed with the molecular markers suggest the rapid evolution of new recombinants of the pathogen in the chickpea growing fields.
authors.affiliation: Genetic Resources and Enhancement Program, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru P.O. A. P. 502 324, India.
authors.forename: S
authors.initials: S
authors.lastname: Sivaramakrishnan
authors.forename: Seetha
authors.initials: S
authors.lastname: Kannan
authors.forename: S D
authors.initials: SD
authors.lastname: Singh
mesh_terms: D015415:Biomarkers; D029863:Cicer / Q000382:microbiology*; D004271:DNA, Fungal / Q000032:analysis; D005670:Fusarium / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D015415:Biomarkers; D004271:DNA, Fungal