Perturbation of protein kinase CK2 uncouples executive part of phosphate maintenance pathway from cyclin-CDK control.
The budding yeast Saccharomyces cerevisiae encounters phosphate starvation by the transcription-regulated PHO pathway. We find that genetic perturbation of protein kinase CK2, a conserved tetrameric Ser/Thr phosphotransferase with links to cell cycle and transcription, affects expression of PHO pathway genes in a subunit- and isoform-specific manner. Remarkably, the genes encoding phosphate supplying phosphatases and transporters are significantly repressed, while the genes encoding components of the central pathway regulator complex, a cyclin-dependent kinase (CDK), a cyclin, and a CDK inhibitor, remain unaltered. Thus, perturbation of CK2 uncouples the executive part of the PHO pathway from its cyclin-CDK control complex.
Barz
Thomas
T
Biochemische Zellphysiologie (A135), Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany.
Ackermann
Karin
K
Pyerin
Walter
W
D001483:Base Sequence; D047390:Casein Kinase II; D002453:Cell Cycle / Q000502:physiology; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D016213:Cyclins / Q000502:physiology*; D017931:DNA Primers; D008954:Models, Biological; D016366:Open Reading Frames; D010710:Phosphates / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology / Q000378:metabolism
D016213:Cyclins; D017931:DNA Primers; D010710:Phosphates; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D018844:Cyclin-Dependent Kinases