title: [Flowcytometry DNA analysis of oral and maxillofacial non-Hodgkin's lymphoma].
abstract: OBJECTIVE
  The purpose of this study was to investigate the relationship between the results of flowcytometry analyses of different clinical stage, location, pathologic grade and cell origin of oral and maxillofacial non-Hodgkin's lymphoma (NHL), and the diagnostic value of flowcytometry analysis in lymphoma.
  
  
  METHODS
  This study analyzed 50 oral and maxillofacial NHL cases and 10 reactive lymph nodes (formalin fixed and paraffin embedded) by flowcytometry (FCM).
  
  
  RESULTS
  Reactive lymph nodes were all diploid. The diploid rate of NHL was 54%, and aneuploidy rate was 46%. There was statistically significant difference between reactive lymph nodes and NHL in the DNA ploidy status and cell cycle data (SPF, CV, S + G2/M, DI). The S phase fraction (SPF) and S + G2/M had close relationship with the grade of NHL. SPF value and DNA ploidy status had no obvious relationship with the prognosis.
  
  
  CONCLUSIONS
  The results suggested that the FCM had diagnostic value in NHL, especially when the morphological diagnosis was difficult. Although the cell cycle data had no prognostic value, SPF and SPF + G2/M can show the proliferative status of NHL, which can help clinical doctor select therapeutic method.
authors.affiliation: College of Stomatology, Zhongshan University.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Zhixiu
authors.initials: Z
authors.lastname: He
authors.forename: Lanyan
authors.initials: L
authors.lastname: Wu
authors.forename: Yixin
authors.initials: Y
authors.lastname: Cai
authors.forename: Hechang
authors.initials: H
authors.lastname: Huang
authors.forename: Song
authors.initials: S
authors.lastname: Lei
mesh_terms: D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000032:analysis*; D005153:Facial Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D008441:Maxillary Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D011003:Ploidies; D011379:Prognosis
chemical_list: D004273:DNA, Neoplasm