[Antimicrobial susceptibility and beta-lactamase types among clinical isolates during January and February 2000 in the Kinki area of Japan].
We studied antimicrobial susceptibility and beta-lactamase types among clinical isolates in the Kinki area of Japan. Eight hundreds isolates of eight organisms were collected by seven medical institutions during January and February 2000. The rates of beta-lactamase producing by using the chromogenic nitrocephin test were 68.0% against Staphylococcus aureus isolates, 6.0% against Haemophilus influenzae isolates, 98.0% against Moraxella catarrhalis isolates. The rate of beta-lactamase negative ampicillin-resistant H. influenzae was 4.0% (4 out of 100). The result of beta-lactamase producing by using the acid-metric method were as follows the penicillinase and cephalosporinase: 27.0% and 37.0% against Escherichia coli isolates, 37.0% and 1.0% against Klebsiella pneumoniae isolates, 21.8% and 100% against Enterobacter cloacae isolates, 24.2% and 96.0% against Serratia marcescens isolates, 7.0% and 22.0% against Pseudomonas aeruginosa isolates. We identified beta-lactamase type of each isolate detected by polymerase chain reaction: SHV-derived extended-spectrum beta-lactamase (ESBLs) (1 isolate of E. coli and 1 isolate of K. pneumoniae), CTX-M-1-derived ESBLs (1 isolate of K. pneumoniae, 1 of E. cloacae and 4 of S. marcescens), and IMP-1-derived metallo beta-lactamases (2 isolates of S. marcescens).
Yamasaki
Katsutoshi
K
Department of Clinical Laboratory, Wakayama Rosai Hospital.
Nishio
Hisaaki
H
Komatsu
Masaru
M
Satoh
Kaori
K
Yamashita
Tomonari
T
Washidu
Ryoudou
R
Shimakawa
Kouichi
K
Kinoshita
Shohiro
S
Ura
Toshiro
T
Aihara
Masanori
M
D001419:Bacteria / Q000187:drug effects* / Q000201:enzymology*; D024881:Drug Resistance, Bacterial; D016972:Enterobacter cloacae / Q000187:drug effects / Q000201:enzymology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D006193:Haemophilus influenzae / Q000187:drug effects / Q000201:enzymology; D007564:Japan; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology; D008826:Microbial Sensitivity Tests; D001936:Moraxella catarrhalis / Q000187:drug effects / Q000201:enzymology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology; D012706:Serratia marcescens / Q000187:drug effects / Q000201:enzymology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology; D001618:beta-Lactamases / Q000096:biosynthesis*
D001618:beta-Lactamases