title: Altered expression of CHL1 by glial cells in response to optic nerve injury and intravitreal application of fibroblast growth factor-2.
abstract: The close homologue of L1 (CHL1) is a member of the L1 family of cell recognition molecules. The protein is expressed by a variety of nerve cell types and subpopulations of glial cells in vivo and promotes elongation of neurites and survival of nerve cells in vitro. Here we demonstrate that glial cells up-regulate expression of CHL1 in response to an intraorbital crush of the adult mouse optic nerve. We also demonstrate that a single intravitreal application of fibroblast growth factor-2 (FGF-2) increases expression of CHL1 in retinal astrocytes and Müller cells. Elevated expression of CHL1 by glial cells in injured optic nerves and astrocytes and Müller cells in FGF-2-treated retinas suggests a role of the protein in the lesioned central nervous system. Results also suggest that trophic factors might exert part of their biological function by modifying expression of cell recognition molecules.
authors.affiliation: Zentrum für Molekulare Neurobiologie, Universität Hamburg, Hamburg, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Rolf
authors.forename: Doris
authors.initials: D
authors.lastname: Lang
authors.forename: Rainer
authors.initials: R
authors.lastname: Hillenbrand
authors.forename: Melanie
authors.initials: M
authors.lastname: Richter
authors.forename: Melitta
authors.initials: M
authors.lastname: Schachner
authors.forename: Udo
authors.initials: U
authors.lastname: Bartsch
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D015815:Cell Adhesion Molecules; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000494:pharmacology*; D015151:Immunoblotting; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D009409:Nerve Crush; D039842:Neural Cell Adhesion Molecule L1 / Q000096:biosynthesis* / Q000172:deficiency; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D020221:Optic Nerve Injuries / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D011506:Proteins; D015854:Up-Regulation
chemical_list: D015815:Cell Adhesion Molecules; C490649:Chl1 protein, mouse; D039842:Neural Cell Adhesion Molecule L1; D011506:Proteins; D016222:Fibroblast Growth Factor 2