Targets of oxidative stress in yeast sod mutants.
Eukaryotic cells have developed mechanisms to rapidly respond towards the environment by changing the expression of a series of genes. There is increasing evidence that reactive oxygen species (ROS), besides causing damage, may also fulfill an important role as second messengers involved in signal transduction. Recently, we have demonstrated that deletion of SOD1 is beneficial for the acquisition of tolerance towards heat and ethanol stresses. The present report demonstrates that a sod1 mutant was the only one capable of acquiring tolerance against a subsequent stress produced by menadione, although this mutant strain had exhibited high sensitivity to oxidative stress. By measuring the level of intracellular oxidation, lipid peroxidation as well as glutathione metabolism, we have shown that in the SOD1-deleted strain, an unbalance occurs in the cell redox status. These results indicated that the capacity of acquiring tolerance to oxidative stress is related to a signal given by one or all of the above factors.
Pereira
M D
MD
Departamento de Bioquímica, Instituto de Química, UFRJ, 21949-900 Rio de Janeiro, Brazil.
Herdeiro
R S
RS
Fernandes
P N
PN
Eleutherio
E C A
EC
Panek
A D
AD
D002470:Cell Survival; D015966:Gene Expression Regulation, Fungal; D005978:Glutathione / Q000032:analysis / Q000378:metabolism; D008668:Metallothionein / Q000096:biosynthesis; D009154:Mutation; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D015290:Second Messenger Systems; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D000072105:Superoxide Dismutase-1; D024483:Vitamin K 3 / Q000494:pharmacology; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D024483:Vitamin K 3; D008668:Metallothionein; D020122:tert-Butylhydroperoxide; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D005978:Glutathione