Brain region-specific changes in the expression of calcium sensor proteins after repeated applications of ketamine to rats.
We investigated the cellular distribution of three calcium sensor proteins, visinin-like protein-1 (VILIP-1), VILIP-3, and hippocalcin, in different rat brain areas after repeated administration of the non-competitive N-methyl-D-aspartate receptor antagonist ketamine. In comparison to controls we observed an increase in the density of VILIP-1 immunoreactive (IR) hippocampal interneurons and presubicular nerve cells in ketamine treated rats, whereas the density of VILIP-1 expressing cells was decreased in the Nuc. accumbens of these rats. No alterations were seen in the distribution patterns of VILIP-3. The density of hippocalcin-expressing neurons was increased in the cingulate cortex of drug-treated rats. Our experiments show that repeated injections of subanesthetic doses of ketamine induce subtle changes in the cellular distribution of calcium sensor proteins which in part resemble those recently described in postmortem brains of human schizophrenics [Bernstein, H.-G., Braunewell, K.-H., Spilker, C., Danos, P., Baumann, B., Funke, S., Diekmann, S., Gundelfinger, E.D. and Bogerts, B., NeuroReport, 13 (2002) 393-396].
Bernstein
Hans-Gert
HG
Department of Psychiatry, University of Magdeburg, Leipziger Strasse 44, D-39120, Magdeburg, Germany. hans-gert.bernstein@medizin.uni-magdeburg.de
Becker
Axel
A
Keilhoff
Gerburg
G
Spilker
Christina
C
Gorczyca
Wojiech A
WA
Braunewell
Karl-Heinz
KH
Grecksch
Gisela
G
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D018691:Excitatory Amino Acid Antagonists; D051597:Hippocalcin; D007150:Immunohistochemistry; D007649:Ketamine; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D051596:Neurocalcin; D009928:Organ Specificity; D051381:Rats; D017207:Rats, Sprague-Dawley; D044169:Receptors, Calcium-Sensing
D002135:Calcium-Binding Proteins; D018691:Excitatory Amino Acid Antagonists; C496693:Hpca protein, rat; C469810:Hpcal1 protein, rat; D009419:Nerve Tissue Proteins; D051596:Neurocalcin; D044169:Receptors, Calcium-Sensing; C496690:Vsnl1 protein, rat; D051597:Hippocalcin; D007649:Ketamine