title: Tc-99m methoxyisobutylisonitrile bone marrow imaging for predicting the levels of myeloma cells in bone marrow in multiple myeloma: correlation with CD38/CD138 expressing myeloma cells.
abstract: The percentage of myeloma cells in bone marrow is subsequently an important index of disease in patients with multiple myeloma (MM). Bone marrow myeloma cells can be detected by strong CD38/CD138 positivity and light scatter characteristics using flow cytometry. The aim of the study was to evaluate the relationship between the degree of Tc-99m methoxyisobutylisonitrile (MIBI) uptake and the percentage of CD38/CD138 expressing myeloma cells in the bone marrow of patients with MM. A total of 15 patients with MM (mean age: 61.7+/-2.4 years; 7 F and 8 M) were included in the study. Tc-99m MIBI imaging was obtained 20 min after injection of 740 MBq Tc-99m MIBI. Planar spot images of the pelvis and thorax were acquired. The uptake of Tc-99m MIBI in the bone marrow was evaluated using a qualitative and also a semiquantitative scoring system for the bone marrow in areas that included the proximal femurs, anterior iliac crest, and sternum. In all patients, flow cytometry was performed for assessing the percentage of CD38/CD138 expressing myeloma cells in the bone marrow samples. There was a statistically significant positive correlation between the percentage of CD38/CD138 expressing plasma cells in bone marrow and both mean qualitative (r=0.689, p=0.005) and semiquantitative (r=0.669, p=0.006) results of Tc-99m MIBI uptake. In conclusion, our results indicate that increased Tc-99m MIBI uptake of bone marrow is related to the percentage of plasma cell infiltration of bone marrow. Tc-99m MIBI bone marrow imaging may be a useful tool for predicting the levels of myeloma cells in bone marrow of patients with MM.
authors.affiliation: Department of Nuclear Medicine, Osmangazi University Medical Faculty, 26480, Eskişchir, Turkey. ilknur_ak@yahoo.com.
authors.forename: I
authors.initials: I
authors.lastname: Ak
authors.affiliation: Department of Hematology, Osmangazi University Medical Faculty, 26480, Eskişchir, Turkey.
authors.forename: V
authors.initials: V
authors.lastname: Aslan
authors.affiliation: Department of Nuclear Medicine, Osmangazi University Medical Faculty, 26480, Eskişchir, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Vardareli
authors.affiliation: Department of Hematology, Osmangazi University Medical Faculty, 26480, Eskişchir, Turkey.
authors.forename: Z
authors.initials: Z
authors.lastname: Gülbaş
mesh_terms: D036541:ADP-ribosyl Cyclase / Q000032:analysis; D051997:ADP-ribosyl Cyclase 1; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers / Q000097:blood; D001853:Bone Marrow / Q000000981:diagnostic imaging* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis; D008875:Middle Aged; D009101:Multiple Myeloma / Q000000981:diagnostic imaging / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000000981:diagnostic imaging; D010950:Plasma Cells / Q000473:pathology; D011237:Predictive Value of Tests; D011509:Proteoglycans / Q000032:analysis; D011877:Radionuclide Imaging; D012720:Severity of Illness Index; D053668:Syndecan-1; D053667:Syndecans; D017256:Technetium Tc 99m Sestamibi / Q000008:administration & dosage / Q000493:pharmacokinetics
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D008562:Membrane Glycoproteins; D011509:Proteoglycans; C508241:SDC1 protein, human; D053668:Syndecan-1; D053667:Syndecans; D017256:Technetium Tc 99m Sestamibi; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1