A novel method to obtain protein release from porous polymer scaffolds: emulsion coating.
To obtain the controlled release of proteins from macro-porous polymeric scaffolds, a novel emulsion-coating method has been developed. In this process, a water-in-oil emulsion, from an aqueous protein solution and a polymer solution, is forced through a prefabricated scaffold by applying a vacuum. After solvent evaporation, a polymer film, containing the protein, is then deposited on the porous scaffold surface. This paper reports the effect of processing parameters on the emulsion coating characteristics, scaffold structure, and protein release and stability. Poly(ether-ester) multiblock copolymers were chosen as the polymer matrix for both scaffolds and coating. Macro-porous scaffolds, with a porosity of 77 vol% and pores of approximately 500 microm were prepared by compression moulding/salt leaching. A micro-porous, homogeneous protein-loaded coating could be obtained on the scaffold surface. Due to the coating, the scaffold porosity was decreased, whereas the pore interconnection was increased. A model protein (lysozyme) could effectively be released in a controlled fashion from the scaffolds. Complete lysozyme release could be achieved within 3 days up to more than 2 months by adjusting the coated emulsion parameters. In addition, the coating process did not reduce the enzymatic activity. This new method appears to be promising for tissue engineering applications.
Sohier
J
J
IsoTis NV, PO Box 98, 3720 AB, Bilthoven, The Netherlands. jerome.sohier@isotis.com
Haan
R E
RE
de Groot
K
K
Bezemer
J M
JM
D000818:Animals; D002645:Chickens; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000379:methods*; D004655:Emulsions; D009113:Muramidase / Q000737:chemistry / Q000493:pharmacokinetics; D011108:Polymers / Q000737:chemistry / Q000493:pharmacokinetics*; D016062:Porosity / Q000187:drug effects; D011506:Proteins / Q000737:chemistry / Q000493:pharmacokinetics*
D003692:Delayed-Action Preparations; D004655:Emulsions; D011108:Polymers; D011506:Proteins; D009113:Muramidase