A new sulfonamide resistance gene (sul3) in Escherichia coli is widespread in the pig population of Switzerland.
A new gene, sul3, which specifies a 263-amino-acid protein similar to a dihydropteroate synthase encoded by the 54-kb conjugative plasmid pVP440 from Escherichia coli was characterized. Expression of the cloned sul3 gene conferred resistance to sulfamethoxazole on E. coli. Two copies of the insertion element IS15Delta/26 flanked the region containing sul3. The sul3 gene was detected in one-third of the sulfonamide-resistant pathogenic E. coli isolates from pigs in Switzerland.
Perreten
Vincent
V
Institute of Veterinary Bacteriology, University of Bern, Switzerland.
Boerlin
Patrick
P
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002352:Carrier Proteins / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000187:drug effects / Q000235:genetics; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D013449:Sulfonamides / Q000494:pharmacology*; D013552:Swine / Q000382:microbiology*; D013557:Switzerland
D000900:Anti-Bacterial Agents; D002352:Carrier Proteins; D004251:DNA Transposable Elements; D013449:Sulfonamides