title: [Prevalence of antibodies against Francisella tularensis in Castilla y León (Spain) before 1997].
abstract: OBJECTIVE
  The aim of this paper was determine the prevalence of antibodies against Francisella tularensis in the representative sample of people from Castilla-León (Spain) before epidemic outbreak of end 1997.
  
  
  METHODS
  We obtain 4,825 sera (between april-1996 and april-1997) of people from Castilla-León. All sera were tested by a microagglutination technique to detect antibodies against Francisella tularensis. The positive sera were tested to determine cross-reaction with Brucella, Yersinia enterocolitica and Proteus in the tube agglutination tests.
  
  
  RESULTS
  We detected antibodies against Francisella tularensis in 9 (0,19%) of the 4,825 sera. Only one serum from the 9 seropositive was positive in the tube agglutination against Brucella. None of the 9 sera were positive against the remaining bacterial antigen tested.
  
  
  CONCLUSIONS
  In the people of Castilla-León before 1997 the prevalence of antibodies against Francisella tularensis was 0,19%.
authors.affiliation: Area de Microbiología. Facultad de Medicina. Universidad de Valladolid. Spain. gutierrez@med.uva.es
authors.forename: M Purificación
authors.initials: MP
authors.lastname: Gutiérrez
authors.forename: Antonio
authors.initials: A
authors.lastname: Orduña
authors.forename: Ana
authors.initials: A
authors.lastname: Dueñas
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Bratos
authors.forename: Ana
authors.initials: A
authors.lastname: Almaraz
authors.forename: Rufino
authors.initials: R
authors.lastname: Alamo
authors.forename: M Jesús
authors.initials: MJ
authors.lastname: Rodríguez Recio
authors.forename: Henar
authors.initials: H
authors.lastname: Rodríguez Marcos
authors.forename: Antonio
authors.initials: A
authors.lastname: Rodríguez Torres
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000097:blood*; D005260:Female; D005604:Francisella tularensis / Q000276:immunology*; D006801:Humans; D008297:Male; D011933:Reagent Kits, Diagnostic; D016036:Seroepidemiologic Studies; D013030:Spain / Q000453:epidemiology; D014406:Tularemia / Q000453:epidemiology* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D011933:Reagent Kits, Diagnostic