title: Identification and functional characterization of Sphingomonas macrogolitabida strain TFA genes involved in the first two steps of the tetralin catabolic pathway.
abstract: Five genes involved in the two initial steps of the tetralin biodegradation pathway of Sphingomonas macrogolitabida strain TFA have been characterized. ThnA1A2 and ThnA3A4, components of the ring-hydroxylating dioxygenase, were encoded in divergently transcribed operons. ThnA1, ThnA2, and ThnA3 were essential for tetralin ring-hydroxylating dioxygenase activity. ThnB was identified as a dehydrogenase required for tetralin biodegradation.
authors.affiliation: Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Seville, Spain.
authors.forename: Emilia
authors.initials: E
authors.lastname: Moreno-Ruiz
authors.forename: María José
authors.initials: MJ
authors.lastname: Hernáez
authors.forename: Olga
authors.initials: O
authors.lastname: Martínez-Pérez
authors.forename: Eduardo
authors.initials: E
authors.lastname: Santero
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D003470:Culture Media; D008969:Molecular Sequence Data; D009876:Operon; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism; D012997:Solvents / Q000378:metabolism*; D020578:Sphingomonas / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D013764:Tetrahydronaphthalenes / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D012997:Solvents; D013764:Tetrahydronaphthalenes; D010088:Oxidoreductases; D010105:Oxygenases; C095210:tetralin