title: Surface expression of inward rectifier potassium channels is controlled by selective Golgi export.
abstract: Traffic of integral membrane proteins along the secretory pathway is not simply a default process but can be selective. Such selectivity is achieved by sequence information within the cargo protein that recruits coat protein complexes to drive the formation of transport vesicles. A number of sequence motifs have been identified in the cytoplasmic domains of ion channels that regulate early trafficking events between the endoplasmic reticulum and the Golgi complex. Here, we demonstrate that the following trafficking step from the Golgi compartment to the plasma membrane can also be selective. The N-terminal domain of the inward rectifier potassium channel Kir2.1 contains specific sequence information that is necessary for its efficient export from the Golgi complex. Lack of this information results in accumulation of the protein within the Golgi and a significant decrease in cell surface expression. As similar results were obtained for the N terminus of another Kir channel subfamily member, Kir4.1, which could functionally substitute for the Kir2.1 N terminus, we propose a more general role of the identified N-terminal domains for post-Golgi trafficking of Kir channels.
authors.affiliation: Department of Physiology II, University of Freiburg, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany. Clemens.Stockklausner@med.uni-heidelberg.de
authors.forename: Clemens
authors.initials: C
authors.lastname: Stockklausner
authors.forename: Nikolaj
authors.initials: N
authors.lastname: Klocker
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D008969:Molecular Sequence Data; D024661:Potassium Channels, Inwardly Rectifying / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport
chemical_list: D024661:Potassium Channels, Inwardly Rectifying