Loss of ligand-binding specificity of fibroblast growth factor receptor 2 by RNA splicing in human chondrosarcoma cells.
We have previously reported the alternatively spliced transcripts of fibroblast growth factor receptor (FGFR) 3 derived by aberrant splicing in human cancers. Here, we describe a novel splice variant of FGFR2 (FGFR2DeltaIII) arising from skipping exons 7-10, resulting in the deletion of Ig-like-III domain in human chondrosarcoma cell. Sf9 cells expressing FGFR2DeltaIII were able to bind FGF1, FGF2, and FGF7, leading to loss of ligand-binding specificity. Together with our previous findings, the present studies suggest that mRNA splicing plays an important role in the regulation of FGFRs' function.
Jang
Jun-Hyeog
JH
Intellectual Biointerface Engineering Center, Seoul National University, 28 Yongon-dong, Chongno-gu, Seoul 110-768, South Korea. juhjang@seoul.com
Chung
Chong-Pyoung
CP
D015153:Blotting, Western; D002813:Chondrosarcoma / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D005091:Exons / Q000235:genetics; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors / Q000378:metabolism; D006493:Heparin / Q000378:metabolism; D006801:Humans; D008024:Ligands; D019000:Phosphotyrosine / Q000378:metabolism; D010957:Plasmids; D011485:Protein Binding; D012326:RNA Splicing / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D051497:Receptor, Fibroblast Growth Factor, Type 2; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017384:Sequence Deletion; D014407:Tumor Cells, Cultured
D017931:DNA Primers; C496453:FGF7 protein, human; D008024:Ligands; D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D051523:Fibroblast Growth Factor 7; D019000:Phosphotyrosine; D005346:Fibroblast Growth Factors; D006493:Heparin; C496353:FGFR2 protein, human; D020794:Receptor Protein-Tyrosine Kinases; D051497:Receptor, Fibroblast Growth Factor, Type 2