A time-lapse capillary assay to study aerotaxis in the archaeon Halobacterium salinarum.
We have developed a method for time-lapse video photography and line scanning of optical densities for analysis of aerotactic responses of Halobacterium salinarum. This automated digital technique, along with line scan analysis of selected frames, gives a unique profile of the aerotactic migration of halobacterial cells.
Boudko
Dmitri
D
The Whitney Laboratory, 9505 Ocean Shore Boulevard, University of Florida, St. Augustine, FL 32080, USA.
Yu
Hyung Suk
HS
Ruiz
Mandelle
M
Hou
Shaobin
S
Alam
Maqsudul
M
D000388:Air; D002197:Capillary Action; D019617:Halobacterium salinarum / Q000502:physiology*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D018715:Microscopy, Video; D009038:Motion; D013997:Time Factors