Identification of 6-methylsulfinylhexyl isothiocyanate as an apoptosis-inducing component in wasabi.
The ethanol extract from Japanese horseradish wasabi was found to inhibit cell proliferation in human monoblastic leukemia U937 cells by inducing apoptotic cell death. Separation by methods including silica gel chromatography and preparative HPLC gave an active compound, which was identified as 6-methylsulfinylhexyl isothiocyanate (6-HITC). Several lines of evidence indicated that 6-HITC induced apoptosis in U937 cells and human stomach cancer MKN45 cells. Thus, 6-HITC is potentially useful as a natural anti-cancer agent.
Watanabe
Makoto
M
School of Food and Nutritional Sciences, University of Shizuoka, and Centre of Excellence for Evolutionary Human Health Sciences, Yada 52-1, Shizuoka 422-8526, Japan.
Ohata
Masahiko
M
Hayakawa
Sumio
S
Isemura
Mamoru
M
Kumazawa
Shigenori
S
Nakayama
Tsutomu
T
Furugori
Michiyo
M
Kinae
Naohide
N
D017209:Apoptosis / Q000187:drug effects*; D002843:Chromatin / Q000648:ultrastructure; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D017879:Isothiocyanates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007948:Leukemia, Monocytic, Acute / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010946:Plants, Medicinal / Q000737:chemistry; D013274:Stomach Neoplasms / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D020298:U937 Cells; D031228:Wasabia / Q000737:chemistry*
C111984:6-methylsulfinylhexyl isothiocyanate; D002843:Chromatin; D017879:Isothiocyanates