The role of nitric oxide in ocular surface diseases.
For the first time, the current series of studies provide a possible pathophysiologic mechanism of NO-induced ocular surface disease. NO is present in tear and aqueous humor and is suspected of having an important physiological role in maintaining normal homeostasis of the ocular surface. NO concentrations are higher in aqueous humor compared to tears, though some variability exists between different species. When inflammation was induced by PTK wounding or LPS, three forms of NOS expression were seen in corneal cells. Each isoform of NOS was expressed uniquely according to the specific location of inflammation. When concentrations of NO peaked, the levels of iNOS were markedly increased in fibroblasts and inflammatory cells. The correlation between NO and inflammation was confirmed by treatment with NOS inhibitor, which abrogated the amount of both NO and inflammation. The tissue damage by NO was measured by nitrotyrosine formation. Damage was detected mainly in inflammatory cells, especially those localized in and around the limbal vessel. It is likely that expression of iNOS in limbal fibroblasts has other roles related to survival of limbal stem cells and fibroblasts as well. Because the main source of NO are fibroblasts, we were able to determine the effect of various concentrations of NO on cell viability using a fibroblast culture system. Cell viability increased in dose dependent manner from 10 microM to 500 microM of the NO generator SNAP, but decreased at concentrations above 1000 microM, suggesting that the in vivo mechanism of cell death was indirect, through specific biologic pathways. Therefore, the pathophysiological mechanism of NO action is bimodal with a toxicological component in ocular surface diseases. Furthermore, its concentration and interaction with other oxygen mediators appear to vary depending on the degree of inflammation.
Kim
J C
JC
Department of Ophthalmology, Chung-Ang University, Seoul, Korea.
Cheong
T B
TB
Park
G S
GS
Park
M H
MH
Kwon
N S
NS
Yoon
H Y
HY
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001082:Aqueous Humor / Q000378:metabolism; D003315:Cornea / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D003316:Corneal Diseases / Q000473:pathology / Q000503:physiopathology*; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D006801:Humans; D009336:Necrosis; D009569:Nitric Oxide / Q000378:metabolism*; D009994:Osmolar Concentration; D030421:Peroxynitrous Acid / Q000096:biosynthesis; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013666:Tears / Q000378:metabolism
D016895:Culture Media, Serum-Free; D030421:Peroxynitrous Acid; D009569:Nitric Oxide