HPLC isolation of the anti-plasmodially active bisbenzylisoquinone alkaloids present in roots of Cissampelos mucronata.
The methanolic extract of dried, powdered Cissampelos mucronata roots possesses significant in vitro activity against Plasmodium falciparum. In order to enable further pharmacological testing, the substances responsible for the observed activity were purified, mainly by HPLC, using various stationary and mobile phases. The active principles were determined to be a series of bisbenzylisoquinoline alkaloids, a group of natural products for which one of the first routine preparative HPLC separation methods is described.
Tshibangu
Jeannette Ndaya
JN
Institute for Pharmaceutical Biology Nussallee 6, D-53115 Bonn, Germany.
Wright
Anthony D
AD
König
Gabriele M
GM
D000470:Alkaloids / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D000962:Antimalarials / Q000302:isolation & purification* / Q000494:pharmacology; D044182:Benzylisoquinolines; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D031606:Cissampelos / Q000737:chemistry*; D007546:Isoquinolines / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000187:drug effects
D000470:Alkaloids; D000962:Antimalarials; D044182:Benzylisoquinolines; D007546:Isoquinolines