Role of prostaglandin E2 in basal and noradrenaline-induced progesterone secretion by the bovine corpus luteum.
The role of prostaglandin E2 (PGE2) in basal and noradrenaline (NA)-stimulated utilization of high density lipoprotein (HDL) as a source of cholesterol for progesterone synthesis was examined. In Experiment 1, a cannula was inserted into the aorta abdominalis through the coccygeal artery (cranial to the origin of the ovarian artery) in mature heifers, to facilitate infusion of NA (4 mg/30 min; n = 3) on day 10 of the estrous cycle. Three other heifers were similarly cannulated to serve as control. Before, during, and after NA or saline infusion, blood samples from the vena cava were collected every 5-15 min for analysis of PGE2, progesterone, and cholesterol. Each NA infusion stimulated (P < 0.01) secretion of both hormones in heifers. Short-duration increases (P < 0.05) in progesterone were observed due to the infusion of NA while cholesterol was not altered significantly. In addition, increases in PGE2 concentrations (P < 0.05) compared to controls were seen after NA infusion. Therefore, we used an in vitro model to verify the effect of PGE2 on HDL utilization by luteal cells from day 5 to 10 of the estrous cycle. In the preliminary experiment, 10(-6) M of PGE2 out of four different doses examined was selected for further studies, since it evoked the highest release of progesterone. In the next experiment, it was found that HDL increases progesterone secretion by luteal cells and both PGE2 and LH increased (P < 0.05) the response to HDL while NA did not. In the last in vitro experiment, progesterone stimulated PGE2 secretion by luteal cells. In conclusion, PGE2 may be directly involved in the utilization of cholesterol from HDL for progesterone synthesis. Furthermore, PGE2 may influence NA-stimulated progesterone secretion by the corpus luteum (CL). It is concluded that there is a positive feedback loop between progesterone and luteal PGE2 during days 5-10 of the estrous cycle.
Kotwica
J
J
Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, 10-718 Olsztyn-Kortowo, Poland. janko@pan.olsztyn.pl
Skarzynski
D
D
Mlynarczuk
J
J
Rekawiecki
R
R
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000818:Animals; D019540:Area Under Curve; D002417:Cattle; D003338:Corpus Luteum / Q000378:metabolism; D015232:Dinoprostone / Q000502:physiology*; D005260:Female; D008075:Lipoproteins, HDL / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism* / Q000494:pharmacology; D011374:Progesterone / Q000378:metabolism; D013997:Time Factors
D000316:Adrenergic alpha-Agonists; D008075:Lipoproteins, HDL; D011374:Progesterone; D015232:Dinoprostone; D009638:Norepinephrine