title: Interaction between Ku80 protein and a widely used antibody to adenomatous polyposis coli.
abstract: The adenomatous polyposis coli (APC) gene and its expressed product are highly studied because of its role as a tumour-suppressor protein. Inherited mutations in APC lead to the condition known as familial adenomatous polyposis (FAP), which predisposes the affected individuals to colorectal cancer. Furthermore, mutations in APC are found in the majority of sporadic cases of colon cancer. There have been many published studies concerning the cellular localisation of APC, this being fundamental to our understanding of its function, but there has also been much concern over the specificity of certain commercially available antibodies to APC. Here we report that the widely used antibody APC(N15) demonstrates a strong interaction with the Ku80 subunit of the Ku heterodimer under defined experimental conditions. Based on the data presented here, we suggest that APC(N15) is not suitable for many applications used for the study of APC.
authors.affiliation: School of Biological Sciences, University of Wales Bangor, Gwynedd, UK.
authors.forename: G T
authors.initials: GT
authors.lastname: Roberts
authors.forename: M L
authors.initials: ML
authors.lastname: Davies
authors.forename: J A
authors.initials: JA
authors.lastname: Wakeman
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000276:immunology* / Q000473:pathology; D025601:Adenomatous Polyposis Coli Protein / Q000276:immunology*; D034961:Antigens, Nuclear / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D015153:Blotting, Western; D003429:Cross Reactions; D004265:DNA Helicases; D004268:DNA-Binding Proteins / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D000072200:Ku Autoantigen; D013058:Mass Spectrometry; D009154:Mutation; D011233:Precipitin Tests; D014407:Tumor Cells, Cultured
chemical_list: D025601:Adenomatous Polyposis Coli Protein; D034961:Antigens, Nuclear; D001324:Autoantigens; D004268:DNA-Binding Proteins; D007074:Immunoglobulin G; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen