Histochemical analysis of phenylphenalenone-related compounds in Xiphidium caeruleum (Haemodoraceae).
Phenylphenalenones represent a typical group of secondary metabolites of the Haemodoraceae. Some of these phenolic compounds show organ-specific distribution within the plant. However, detailed information on cellular localisation is still lacking. To this end, confocal laser-scanning microscopy, microspectral photometry and high-performance liquid chromatography were used to study the tissue localisation of phenylphenalenone-type compounds in Xiphidium caeruleum Aubl. From the autofluorescence potential of these compounds, specific distribution of allophanylglucosides and non-glucosidic compounds of the phenylphenalenone-type in distinct cells of the roots (apical meristem, cortex, cap, epidermis) and the shoot system was revealed. Fluorescence enhancement using "Naturstoff reagent A" (NA) indicated the occurrence of NA-positive natural products in the vacuoles of leaf epidermal cells. The present results provide new insights into the possible functions of phenylphenalenone-related compounds in the context of their localisation. Additionally, the advantages and limitations of the techniques are discussed.
Opitz
S
S
Max-Planck-Institute for Chemical Ecology, Beutenberg Campus, Winzerlaer Str. 10, 07745 Jena, Germany.
Schnitzler
J-P
JP
Hause
B
B
Schneider
B
B
D002109:Caffeic Acids / Q000032:analysis*; D002911:Chrysenes / Q000032:analysis*; D003373:Coumaric Acids / Q000032:analysis*; D003374:Coumarins / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods; D019684:Magnoliopsida / Q000737:chemistry*; D018613:Microscopy, Confocal; D043803:Phenalenes; D010636:Phenols / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D011083:Polycyclic Compounds / Q000032:analysis*; D020005:Propanols / Q000032:analysis; D013644:Tartrates / Q000032:analysis*
C474563:5-hydroxy-2-methoxy-6-oxabenzo(def)chrysen-1-one; C474562:6-O-((6''-O-allophanyl)glucopyranosyl)-5-hydroxy-2-methoxy-7-phenylphenalen-1-one; C474561:6-O-((6''-O-allophanyl)glucopyranosyl)-5-methoxy-7-phenyl-3H-benzo(de)isochromen-1-one; C474560:6-hydroxy-5-methoxy-7-phenyl-3H-benzo(de)isochromen-1-one; D002109:Caffeic Acids; D002911:Chrysenes; D003373:Coumaric Acids; D003374:Coumarins; D005960:Glucosides; D043803:Phenalenes; D010636:Phenols; D011083:Polycyclic Compounds; D020005:Propanols; D013644:Tartrates; C474564:caffeoyl tartaric acid; C474565:feruloyl tartaric acid; C439395:1-phenylpropanol; C038036:phenalen-1-one