title: Competitive interactions of adriamycin and ethidium bromide with DNA as studied by full rank parallel factor analysis of fluorescence three-way array data.
abstract: The competitive interactions of adriamycin (AMC) and a fluorescence probe of ethidium bromide (EB) with DNA have been studied by full rank parallel factor analysis (FRA-PARAFAC) of fluorescence excitation-emission three-way data array. The excitation and emission spectra as well as the equilibrium concentrations of co-existing species in different reaction mixtures can be directly obtained by the FRA-PARAFAC treatment. The concordance of the resolved excitation and emission spectra of AMC, EB and EB-DNA with the standard spectra of these species confirmed the reliability of the equilibrium concentrations of these components in the reaction mixtures studied. The results obtained are valuable for providing a deeper insight into the competitive interaction mechanism of AMC and EB with DNA. The conclusion was directly given out that the interaction of AMC with DNA is the intercalating model. The FRA-PARAFAC method as exemplified by the present study provides an useful approach for studying the interaction of clinical drugs with DNA in the presence of disturbance of drug assistants.
authors.affiliation: State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemometrics and Chemical Engineering, Institute for Chemometrics and Chemical Technology, Hunan University, Changsha 410082, People's Republic of China.
authors.forename: Hong-Ping
authors.initials: HP
authors.lastname: Xie
authors.forename: Xia
authors.initials: X
authors.lastname: Chu
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Jiang
authors.forename: Hui
authors.initials: H
authors.lastname: Cui
authors.forename: Guo-Li
authors.initials: GL
authors.lastname: Shen
authors.forename: Ru-Qin
authors.initials: RQ
authors.lastname: Yu
mesh_terms: D000465:Algorithms; D000970:Antineoplastic Agents / Q000737:chemistry; D001667:Binding, Competitive; D004247:DNA / Q000378:metabolism*; D004317:Doxorubicin / Q000737:chemistry* / Q000378:metabolism; D004996:Ethidium / Q000737:chemistry* / Q000378:metabolism; D007364:Intercalating Agents / Q000494:pharmacology; D015233:Models, Statistical; D013050:Spectrometry, Fluorescence
chemical_list: D000970:Antineoplastic Agents; D007364:Intercalating Agents; D004317:Doxorubicin; D004247:DNA; D004996:Ethidium