title: Type II collagen and aggrecan mRNA expression by in situ hybridization in rabbit temporomandibular joint posterior attachment following disc displacement.
abstract: Pathological changes and mRNA expression were studied in the posterior attachment of 40 adult Japanese white rabbits. The right temporomandibular joints of 28 rabbits were subjected to surgical disc displacement. Joints were studied by histochemistry and in situ hybridization. The collagen in the posterior attachment became dense, especially near the posterior band of the disc. Chondrocytes were found not only in the disc but also in the posterior attachment. Sometimes cartilage formation was seen. Type II collagen mRNA expression was first detected in the posterior attachment 4 days postoperatively and became progressively stronger with time. Aggrecan expression in the posterior attachment decreased at first, then increased gradually. It was concluded that, in the temporomandibular joint, chondrocytes appear in the posterior attachment as a result of biomechanical stimuli and the attachment becomes fibrocartilaginous following disc displacement.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Zhejiang University, #395 Yan'an Street, Zhejiang 310006, Hangzhou, PR China. gzy@zju.edu.cn <gzy@zju.edu.cn>
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Gu
authors.forename: Jianying
authors.initials: J
authors.lastname: Feng
authors.forename: Takanori
authors.initials: T
authors.lastname: Shibata
authors.forename: Ji'an
authors.initials: J
authors.lastname: Hu
authors.forename: Zhenkang
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D053674:Aggrecans; D000818:Animals; D019902:Chondrocytes / Q000473:pathology; D024043:Collagen Type II / Q000096:biosynthesis* / Q000235:genetics; D016326:Extracellular Matrix Proteins; D005260:Female; D015870:Gene Expression; D017403:In Situ Hybridization; D004204:Joint Dislocations / Q000378:metabolism* / Q000473:pathology; D037181:Lectins, C-Type; D008297:Male; D011509:Proteoglycans / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D013704:Temporomandibular Joint / Q000378:metabolism* / Q000473:pathology; D019224:Temporomandibular Joint Disc / Q000473:pathology; D013705:Temporomandibular Joint Disorders / Q000378:metabolism* / Q000473:pathology
chemical_list: D053674:Aggrecans; D024043:Collagen Type II; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011509:Proteoglycans; D012333:RNA, Messenger