[Expression and significance of B-cell-specific activator protein of H/RS cell in classical Hodgkin's Lymphoma].
OBJECTIVE
To explore the expression of B-cell-specific activator protein (BSAP) of H/RS cell in classical Hodgkin's lymphoma (cHL).


METHODS
Immunohistochemical method was used to detect the expression of BSAP in 33 samples of formalin-fixed, paraffin-embedded tissues of cHL. Nine samples of lymph node of reactive hyperplasia, 10 samples of B-cell lymphoma, and 10 samples of T-cell lymphoma were also detected as BSAP controls. Mouse-anti-human monoclonal antibodies CD20, CD30 and CD15 were detected among the cHL cases as routine comparison.


RESULTS
30 of 33 (90.91%) cases of cHL were BSAP expression positive in H/RS cells, while all of the 33 (100%) cases of background B-lymphocytes were BSAP positive. Almost all B cells of lymph node reactive hyperplasia were BSAP positive. All malignant cells in B-cell lymphoma were BSAP positive, while all malignant cells in T-cell lymphoma were BSAP negative. Among the 33 cases of cHL there was a significant difference between the expression of BSAP and the expression of CD20 (30.30%) in H/RS cells (P = 0.000), and no significant difference between the expression of CD30 (93.94%) and CD15 (75.75%, P = 0.082).


CONCLUSIONS
The frequent expression of BSAP in H/RS cells of classical Hodgkin's disease provides further evidence for its B-cell origin and helps to identify H/RS cells. The expression of BSAP in H/RS cells can be used to distinguish HL from anaplastic large cell lymphoma (ALCL).
Zhou
Xinhua
X
Department of Pathology, First Military Medical University, Guangzhou 510515, China.
Zhao
Tong
T
Qi
Zongli
Z
Liu
Gang
G
Zhu
Meigang
M
D000293:Adolescent; D000328:Adult; D018951:Antigens, CD20 / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D003937:Diagnosis, Differential; D005260:Female; D006689:Hodgkin Disease / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D017730:Ki-1 Antigen / Q000032:analysis; D016256:Lewis X Antigen / Q000032:analysis; D017728:Lymphoma, Large-Cell, Anaplastic / Q000378:metabolism / Q000473:pathology; D008297:Male; D051757:PAX5 Transcription Factor; D016539:Reed-Sternberg Cells / Q000737:chemistry / Q000473:pathology*; D014157:Transcription Factors / Q000096:biosynthesis*
D018951:Antigens, CD20; D004268:DNA-Binding Proteins; D017730:Ki-1 Antigen; D016256:Lewis X Antigen; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human; D014157:Transcription Factors