Cell type specific targeted intracellular delivery into muscle of a monoclonal antibody that binds myosin IIb.
Methods for cell type specific targeted intracellular delivery of proteins in vivo remain limited. A murine monoclonal anti-dsDNA antibody, mAb 3E10, was selectively transported into skeletal muscle cells in vivo. The antibody bound a 200 kDa protein only found in lysates of skeletal muscle by Western blotting. The 200 kDa protein was purified from muscle lysate by antibody affinity chromatography and identified as the skeletal muscle specific heavy chain of myosin IIb by electrospray mass spectrometry. Antibody binding specificity for myosin IIb was demonstrated in Western blots by binding myosin in skeletal muscle lysates from mice null for myosin IId but not in mice null for myosin IIb. Myosin IIb is implicated in the specific targeting of mAb 3E10 to skeletal muscle.
Weisbart
Richard H
RH
Department of Medicine, Division of Rheumatology, Veterans Affairs Greater Los Angeles Health Care System, Sepulveda, CA 91343, USA. rweisbar@ucla.edu
Yang
Fusheng
F
Chan
Grace
G
Wakelin
Rika
R
Ferreri
Kevin
K
Zack
Debra J
DJ
Harrison
Brooke
B
Leinwand
Leslie A
LA
Cole
Greg M
GM
D000595:Amino Acid Sequence; D000818:Animals; D000974:Antibodies, Antinuclear / Q000008:administration & dosage / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000493:pharmacokinetics; D000918:Antibody Specificity; D005260:Female; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000276:immunology* / Q000378:metabolism; D024501:Nonmuscle Myosin Type IIB / Q000172:deficiency / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D014018:Tissue Distribution
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D024501:Nonmuscle Myosin Type IIB