Incorporation and accumulation of docosahexaenoic acid from the medium by Pichia methanolica HA-32.
Yeast species were screened for the incorporation and accumulation of docosahexaenoic acid (DHA) with a yeast-malt medium containing 0.5% free fatty acid prepared from fish oil (DHA, 28% of total fatty acids in fish oil). The most suitable strain was Pichia methanolica HA-32. The optimum cultivation conditions for the accumulation of lipids and incorporation of DHA were as follows: 5% glucose, 20% yeast extract, and 3% free fatty acid in the medium, at pH 6.0 and with incubated at 25 degrees C for 3 days. Under these conditions, about 200 mg of total lipids and 60 mg of DHA were recovered from 1 g of dry cells. The accumulation of DHA in cells increased in conjunction with the amount of yeast extract added to the medium. Vitamin B groups and minerals also had an effect on the accumulation of DHA. Choline and K2HPO4, which caused browning of the medium, promoted the accumulation of DHA in cells.
Aoki
Hideyuki
H
Research Laboratory, Ikeda Food Research Co., Ltd., 95-7 Minooki-Cho, Fukuyama, Hiroshima 721-0956, Japan.
Miyamoto
Noriko
N
Furuya
Yuji
Y
Mankura
Mitsumasa
M
Endo
Yasushi
Y
Fujimoto
Kenshiro
K
D002244:Carbon / Q000032:analysis; D002794:Choline / Q000032:analysis / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D050356:Lipid Metabolism; D009584:Nitrogen / Q000032:analysis; D010843:Pichia / Q000145:classification / Q000166:cytology / Q000378:metabolism*; D012972:Sodium Hydroxide / Q000032:analysis / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014803:Vitamin B Complex / Q000032:analysis / Q000378:metabolism
D003470:Culture Media; D005230:Fatty Acids, Nonesterified; D014803:Vitamin B Complex; D004281:Docosahexaenoic Acids; D012972:Sodium Hydroxide; D002244:Carbon; D005947:Glucose; D009584:Nitrogen; D002794:Choline