title: [Cloning of transmembrane domain sequence of EGFR gene]
abstract: OBJECTIVE: To clone the transmembrane (TM) domain sequence of EGFR gene and lay a good foundation for constructing the transmembrane expression vector of recombinant superantigens and cytokines. METHODS: A pair of primers special to the sequence encoding TM domain of EGFR gene were synthesized, TM domain fragment was cloned by RT-PCR, and the PCR product of TM domain sequence was ligated with the pGEM-T vector and confirmed by DNA sequencing. RESULTS: TM domain sequence was successfully cloned and verified by DNA sequencing. CONCLUSION: The successful cloning of TM domain sequence provides a basis for the construction of transmembrane fusion protein of Superantigen-TM or Cytokines-TM in cancer biotherapy.
authors.affiliation: Cancer Institute, College of Medical Sciences, Zhejiang University, Hangzhou 310009, China.
authors.forename: Wen-Xue
authors.initials: WX
authors.lastname: Ma
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.forename: Ping-Yong
authors.initials: PY
authors.lastname: Yi
authors.forename: Hong-Chuan
authors.initials: HC
authors.lastname: Jin
authors.forename: Jie
authors.initials: J
authors.lastname: Yan