title: Assessing gene expression variation in normal human tissues using GeneTag, a novel, global, sensitive profiling method.
abstract: GeneTag is a novel expression profiling method that allows the visualization, quantification and identification of expressed genes-whether known or novel-in any species, tissue or cell type, independent of knowledge of the underlying sequence. Here we describe the application of this method to determine variation of gene expression in individual human liver samples and the identification of tissue-specific genes by comparing expression patterns across several human organs. Expression data are stored in a database for future reference and data analysis relies on proprietary software, which allows complex comparisons to be performed. Differentially expressed genes are quickly identified through a link to a sequence database. The results from our study underscore the importance of knowledge of individual variation of gene expression for the design and interpretation of transcript profiling experiments in the context of any biological question.
authors.affiliation: Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA.
authors.forename: Lily Y
authors.initials: LY
authors.lastname: Wong
authors.forename: Andrea
authors.initials: A
authors.lastname: Hafeman
authors.forename: Victoria L
authors.initials: VL
authors.lastname: Boyd
authors.forename: John
authors.initials: J
authors.lastname: Bodeau
authors.forename: Katherine D
authors.initials: KD
authors.lastname: Lazaruk
authors.forename: Sueh-Ning
authors.initials: SN
authors.lastname: Liew
authors.forename: Peter
authors.initials: P
authors.lastname: Casey
authors.forename: Victor
authors.initials: V
authors.lastname: Belonogoff
authors.forename: Somaya
authors.initials: S
authors.lastname: Bit
authors.forename: Craig
authors.initials: C
authors.lastname: Sumner
authors.forename: Alisa
authors.initials: A
authors.lastname: Bredo
authors.forename: Norman
authors.initials: N
authors.lastname: Ho
authors.forename: Erwin
authors.initials: E
authors.lastname: Chu
authors.forename: Sheri
authors.initials: S
authors.lastname: Olson
authors.forename: Steven
authors.initials: S
authors.lastname: Rabkin
authors.forename: Sergei
authors.initials: S
authors.lastname: Maltchenko
authors.forename: Gene
authors.initials: G
authors.lastname: Spier
authors.forename: Dennis
authors.initials: D
authors.lastname: Gilbert
authors.forename: Susanne
authors.initials: S
authors.lastname: Baumhueter
mesh_terms: D001921:Brain; D001923:Brain Chemistry; D016172:DNA Fingerprinting / Q000379:methods; D018076:DNA, Complementary / Q000032:analysis; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008099:Liver / Q000737:chemistry* / Q000196:embryology; D008168:Lung / Q000737:chemistry; D008297:Male; D009928:Organ Specificity; D010920:Placenta / Q000737:chemistry; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016415:Sequence Alignment / Q000379:methods*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger