Mycobacteria-containing phagosomes associate less annexins I, VI, VII and XI, but not II, concomitantly with a diminished phagolysosomal fusion.
We have studied the intracellular localization of annexins I,II, VI, VII, and XI in cells containing latex beads or Mycobacterium avium at different times after ingestion in order to establish whether a correlation existed between the association of annexins to phagosomes and phagolysosomal fusion, since the intracellular survival of mycobacteria is linked to an impairment of phagosome maturation. We demonstrate an important decrease in the levels of association of annexins I, VI, VII and XI, but not II to phagosomes containing either live or killed mycobacteria compared with phagosomes containing inert latex particles. The reduced association of annexins observed was detected only on M. avium-containing phagosomes and not in other cell membrane nor in cytosolic fractions from infected cells, and was apparent from 8 hours through to 4 days after phagocytosis. These findings add elements to the present knowledge of the phagosomal modifications that accompany the survival of intracellular pathogens, suggesting that annexins I, VI, VII, and XI play a secondary role in phagosomal fusion events while annexin II does not seem to be related to the mechanism of regulation of endolysosomal fusion.
Pittis
Maria G
MG
Leukocyte Biology Unit, International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
Muzzolin
Laura
L
Giulianini
Piero G
PG
Garcia
Rodolfo C
RC
D017305:Annexin A1 / Q000378:metabolism; D017317:Annexin A6 / Q000378:metabolism; D017310:Annexin A7 / Q000378:metabolism; D017302:Annexins / Q000378:metabolism*; D002470:Cell Survival / Q000502:physiology; D006790:Host-Parasite Interactions / Q000502:physiology; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D008561:Membrane Fusion / Q000502:physiology*; D008863:Microspheres; D009164:Mycobacterium Infections / Q000378:metabolism; D009162:Mycobacterium avium / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D015388:Organelles / Q000378:metabolism / Q000382:microbiology / Q000648:ultrastructure; D010587:Phagocytosis / Q000502:physiology; D010588:Phagosomes / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000378:metabolism / Q000382:microbiology / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
D017305:Annexin A1; D017317:Annexin A6; D017310:Annexin A7; D017302:Annexins