title: Asymmetric inheritance of oxidatively damaged proteins during cytokinesis.
abstract: Carbonylated proteins were visualized in single cells of the budding yeast Saccharomyces cerevisiae, revealing that they accumulate with replicative age. Furthermore, carbonylated proteins were not inherited by daughter cells during cytokinesis. Mother cells of a yeast strain lacking the sir2 gene, a life-span determinant, failed to retain oxidatively damaged proteins during cytokinesis. These findings suggest that a genetically determined, Sir2p-dependent asymmetric inheritance of oxidatively damaged proteins may contribute to free-radical defense and the fitness of newborn cells.
authors.affiliation: Department of Cell and Molecular Biology-Microbiology, Göteborg University, Göteborg, Sweden.
authors.forename: Hugo
authors.initials: H
authors.lastname: Aguilaniu
authors.forename: Lena
authors.initials: L
authors.lastname: Gustafsson
authors.forename: Michel
authors.initials: M
authors.lastname: Rigoulet
authors.forename: Thomas
authors.initials: T
authors.lastname: Nyström
mesh_terms: D000199:Actins / Q000378:metabolism; D002455:Cell Division; D005800:Genes, Fungal; D006655:Histone Deacetylases / Q000235:genetics / Q000502:physiology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007150:Immunohistochemistry; D008928:Mitochondria / Q000378:metabolism; D009154:Mutation; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010269:Paraquat / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology; D056565:Sirtuin 2; D037761:Sirtuins / Q000235:genetics / Q000502:physiology; D013481:Superoxides / Q000378:metabolism
chemical_list: D000199:Actins; D017382:Reactive Oxygen Species; C084122:SIR3 protein, S cerevisiae; C061573:SIR4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; D013481:Superoxides; D006861:Hydrogen Peroxide; C087778:SIR2 protein, S cerevisiae; D056565:Sirtuin 2; D037761:Sirtuins; D006655:Histone Deacetylases; D010269:Paraquat