title: Presence and localization of CCK receptor subtypes in calf pancreas.
abstract: This study was undertaken to confirm the presence of CCK receptor subtypes in calf pancreas and establish their cellular localization. Using specific antibodies against CCKA and CCKB receptors, somatostatin, glucagon and insulin, we were able to confirm by Western blot the presence of both CCK receptor protein subtypes in the calf pancreas as a 80-85-kDa CCKA receptor and 40-45-kDa CCKB receptor. By immunofluorescence, the CCKB receptor colocalizes with the islets' somatostatin delta cells, confirming what was previously shown in other species, as well as on ductal cells. We could not reproduce in the calf its colocalization with glucagon alpha cells as observed in human and rat. Any specific localization of CCKA receptors with our multiple antibodies failed. Our observation that the CCKB receptor subtype is specifically localized on pancreatic delta cells as well as on ductal cells lets us support the hypothesis that in this species, CCK could be involved in somatostatin metabolism as well as hydrelatic secretion; its effect on enzyme secretion would be indirect.
authors.affiliation: Service de gastroentérologie, Dép de médecine et pathologie, Faculté de médecine, Université de Sherbrooke, 3001-12ième Avenue Nord, Sherbrooke, Quebec, Canada J1H 5N4. Jean.Morisset@USherbrooke.ca
authors.forename: Jean
authors.initials: J
authors.lastname: Morisset
authors.forename: Jean
authors.initials: J
authors.lastname: Lainé
authors.forename: Judith
authors.initials: J
authors.lastname: Bourassa
authors.forename: Michel
authors.initials: M
authors.lastname: Lessard
authors.forename: Véronique
authors.initials: V
authors.lastname: Rome
authors.forename: Paul
authors.initials: P
authors.lastname: Guilloteau
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002417:Cattle / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005934:Glucagon / Q000302:isolation & purification; D007150:Immunohistochemistry; D007328:Insulin / Q000302:isolation & purification; D007515:Islets of Langerhans / Q000378:metabolism; D007633:Keratins / Q000302:isolation & purification; D008297:Male; D010179:Pancreas / Q000166:cytology / Q000378:metabolism*; D011949:Receptors, Cholecystokinin / Q000145:classification / Q000378:metabolism*; D013004:Somatostatin / Q000302:isolation & purification
chemical_list: D007328:Insulin; D011949:Receptors, Cholecystokinin; D013004:Somatostatin; D007633:Keratins; D005934:Glucagon