title: Tyrosinase gene correction using fluorescent oligonucleotides.
abstract: Gene therapy and production of mutated cell lines or animal models should be improved significantly once efficient controlled gene targeting strategies are developed. We used short single-stranded oligodeoxynucleotides (ODN), in some cases coupled to the fluorescent dye fluorescein isothiocyanate (FITC), to correct an endogenic natural point mutation in melanocytes in culture. The addition of the FITC molecule to the 5' extremity of the ODN did not interfere with the efficiency of the reversion of the mutation and did not have any deleterious side-effects. The use of fluorescent ODN could lead to great improvement in the technique. In particular, it may facilitate sorting of the transfected cells in the treated population, and thereby significantly increase the percentage of corrected cells.
authors.affiliation: Recombination and Genomic Instability, UMR 2027 CNRS-Institut Curie, Orsay, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Biet
authors.forename: Christophe
authors.initials: C
authors.lastname: Alberti
authors.forename: Paolo
authors.initials: P
authors.lastname: Faccella
authors.forename: Jian Sheng
authors.initials: JS
authors.lastname: Sun
authors.forename: Marie
authors.initials: M
authors.lastname: Dutreix
authors.forename: Lionel
authors.initials: L
authors.lastname: Larue
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000737:chemistry; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D016650:Fluorescein-5-isothiocyanate / Q000494:pharmacology* / Q000627:therapeutic use; D018390:Gene Targeting / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D014442:Monophenol Monooxygenase / Q000235:genetics*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology* / Q000627:therapeutic use; D017354:Point Mutation / Q000187:drug effects* / Q000235:genetics; D014162:Transfection / Q000379:methods
chemical_list: D015415:Biomarkers; D009838:Oligodeoxyribonucleotides; D014442:Monophenol Monooxygenase; D016650:Fluorescein-5-isothiocyanate