title: Number of Langerhans immune cells in painful and non-painful human skin after shingles.
abstract: During injury or inflammation, paracrine sensitization of peripheral sensory neurons by immune cells contributes to the sensation of pain. It is less clear whether this neural sensitization contributes to neuropathic pain after neural injury as well. Shingles (herpes zoster) is a common disease that leaves some patients with prolonged neuropathic pain known as postherpetic neuralgia (PHN). Sensitization of cutaneous neurons has been hypothesized to contribute to PHN. Langerhans cells (LC), the Ia(+) macrophages of the skin, contact epidermal neurites and, when activated, synthesize molecules with the ability to sensitize axons. For these reasons, we examined morphological evidence for activation of LC in subjects with established PHN. We also evaluated the relationship between numbers of LC and nociceptive epidermal nerve endings; these are markedly reduced in PHN. We used design-based stereology to estimate the number of CD1a(+) LC in biopsies of painful and non-painful skin from ten adults with or without PHN after shingles on the torso. There were no differences in the number of LC in previously shingles-affected and normal-control skin biopsies. The number of LC also remained at normal levels in biopsies with near-loss of innervation from shingles. LC numbers were unrelated to the presence or severity of pain. These data suggest that neuropathic pain in established PHN is not associated with increased numbers of cutaneous macrophages, and that the number of cutaneous macrophages in skin from the human torso is independent of the number of epidermal nerve endings.
authors.affiliation: Department of Neurosurgery, Johns Hopkins Medical Institutions, Baltimore, MD, USA.
authors.forename: Anne Louise
authors.initials: AL
authors.lastname: Oaklander
authors.forename: E Adelaine
authors.initials: EA
authors.lastname: Stocks
authors.forename: Peter R
authors.initials: PR
authors.lastname: Mouton
mesh_terms: D000368:Aged; D002452:Cell Count; D005260:Female; D006562:Herpes Zoster / Q000150:complications / Q000473:pathology* / Q000503:physiopathology; D006801:Humans; D007801:Langerhans Cells / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009437:Neuralgia / Q000209:etiology / Q000473:pathology; D010146:Pain / Q000473:pathology / Q000503:physiopathology; D012867:Skin / Q000294:innervation / Q000473:pathology / Q000503:physiopathology