Bone marrow transplantation abolishes inhibition of arteriogenesis in placenta growth factor (PlGF) -/- mice.
We studied the influence of placenta growth factor (PlGF) on arteriogenesis and tested the role of bone marrow (BM)-derived cells on PlGF-depleted vascular growth.


METHODS
Right femoral artery was occluded in wild type (+/+), PlGF k.o. (-/-) and (-/-) mice receiving BM from (+/+) mice (-/- BM). Blood supply in paws was assessed by laser-Doppler imaging (LDI) measurements before, immediately after, as well as 3, 7, 14, 21 and 28 days after surgery. Seven and 28 days after occlusion, hindlimbs were perfusion fixed and filled with contrast medium. Angiograms were evaluated, collateral arteries were analysed histologically and morphometrically.


RESULTS
Seven days after occlusion the blood flow and collateral growth in (-/-) were delayed in comparison to the (+/+) group. BM transplantation from +/+ donors prevented this delay. After 3 weeks the (-/-) mice reached values similar to the +/+ control.


CONCLUSIONS
PlGF is an important promoter of arteriogenesis. BM transplantation abolishes inhibition of arteriogenesis in PlGF -/- mice.
Scholz
Dimitri
D
Department of Experimental Cardiology, Max-Planck-Institute, Benekestrasse 2, D-61231 Bad Nauheim, Germany. d.scholz@kerckhoff.mpg.de
Elsaesser
Hanno
H
Sauer
Annegret
A
Friedrich
Christian
C
Luttun
Aernout
A
Carmeliet
Peter
P
Schaper
Wolfgang
W
D000792:Angiography; D000818:Animals; D001158:Arteries / Q000033:anatomy & histology / Q000502:physiology*; D016026:Bone Marrow Transplantation; D003097:Collateral Circulation; D005121:Extremities / Q000098:blood supply; D005263:Femoral Artery / Q000033:anatomy & histology / Q000000981:diagnostic imaging; D017078:Laser-Doppler Flowmetry; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009000:Monocytes / Q000737:chemistry; D000072483:Placenta Growth Factor; D011257:Pregnancy Proteins / Q000235:genetics / Q000502:physiology*; D012039:Regional Blood Flow
C000608037:Pgf protein, mouse; D011257:Pregnancy Proteins; D000072483:Placenta Growth Factor