[Cloning and identification of 5'-untranslated region (UTR) and 3'-untranslated region of neutrophil gelatinase-associated lipocalin (NGAL) gene from esophageal carcinoma cell line SHEEC].
OBJECTIVE
Neutrophil gelatinase-associated lipocalin (NGAL) was a novel member of the lipocalin family. The authors previously found that NGAL was overexpressed in the progress of malignant transformation from human immortalized esophageal epithelial cell line SHEE to esophageal carcinoma cell line SHEEC. However, the regulation mechanism of NGAL overexpression was not known. The objective of this study was to clone 5'-untranslated region(5'-UTR) and 3'untranslated region (3'-UTR) of NGAL in SHEEC and to analyze their structural characters.


METHODS
5'-UTR and 3'-UTR of NGAL were cloned from SHEEC using rapid amplification of cDNA ends(RACE). After sequencing the alignment of their nucleotides was analyzed by BLAST database of NCBI and the potential cis-acting elements in the 3'-UTR were identified by computer analysis.


RESULTS
The authors cloned and sequenced 69 bp 5'-UTR and 147 bp 3'-UTR of NGAL gene on the basis of the previous works and did not find any base pair mutation.


CONCLUSIONS
NGAL gene from SHEEC had the entire 5'-UTR and 3'-UTR.
Li
En-Min
EM
Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou, Guangdong, 515031, PR China. nmli@21cn.com
Xu
Li-Yan
LY
Xiong
Hua-Qi
HQ
Cai
Wei-Jia
WJ
Wu
Bing-Li
BL
Zhang
Can
C
Zhang
Yong-Fa
YF
Lin
Yan
Y
Shen
Zhong-Ying
ZY
D020413:3' Untranslated Regions / Q000235:genetics* / Q000302:isolation & purification; D020121:5' Untranslated Regions / Q000235:genetics* / Q000302:isolation & purification; D000209:Acute-Phase Proteins; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004938:Esophageal Neoplasms / Q000235:genetics*; D006801:Humans; D000071068:Lipocalin-2; D054834:Lipocalins; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D012334:RNA, Neoplasm / Q000032:analysis; D014407:Tumor Cells, Cultured
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000209:Acute-Phase Proteins; D002352:Carrier Proteins; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D012334:RNA, Neoplasm