title: Anion-dependent Mg2+ influx and a role for a vacuolar H+-ATPase in sheep ruminal epithelial cells.
abstract: The K+-insensitive component of Mg2+ influx in primary culture of ruminal epithelial cells (REC) was examined by means of fluorescence techniques. The effects of extracellular anions, ruminal fermentation products, and transport inhibitors on the intracellular free Mg2+ concentration ([Mg2+]i), Mg2+ uptake, and intracellular pH were determined. Under control conditions (HEPES-buffered high-NaCl medium), the [Mg2+]i of REC increased from 0.56 +/- 0.14 to 0.76 +/- 0.06 mM, corresponding to a Mg2+ uptake rate of 15 microM/min. Exposure to butyrate did not affect Mg2+ uptake, but it was stimulated (by 84 +/- 19%) in the presence of CO2/HCO(-)3. In contrast, Mg2+ uptake was strongly diminished if REC were suspended in HCO(-)3-buffered high-KCl medium (22.3 +/- 4 microM/min) rather than in HEPES-buffered KCl medium (37.5 +/- 6 microM/min). After switching from high- to low-Cl- solution, [Mg2+]i was reduced from 0.64 +/- 0.09 to 0.32 +/- 0.16 mM and the CO2/HCO(-)3-stimulated Mg2+ uptake was completely inhibited. Bumetanide and furosemide blocked the rate of Mg2+ uptake by 64 and 40%, respectively. Specific blockers of vacuolar H+-ATPase reduced the [Mg2+]i (36%) and Mg2+ influx (38%) into REC. We interpret this data to mean that the K+-insensitive Mg2+ influx into REC is mediated by a cotransport of Mg2+ and Cl- and is energized by an H+-ATPase. The stimulation of Mg2+ transport by ruminal fermentation products may result from a modulation of the H+-ATPase activity.
authors.affiliation: Institute for Veterinary Physiology, Free University of Berlin, Oertzenweg 19b, 14163 Berlin, Germany. shweigel@zedat.fu-berlin.de
authors.forename: Monika
authors.initials: M
authors.lastname: Schweigel
authors.forename: Holger
authors.initials: H
authors.lastname: Martens
mesh_terms: D000818:Animals; D000838:Anions / Q000493:pharmacokinetics; D001639:Bicarbonates / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002087:Butyrates / Q000494:pharmacology; D002245:Carbon Dioxide / Q000378:metabolism; D002478:Cells, Cultured; D002712:Chlorides / Q000493:pharmacokinetics*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000493:pharmacokinetics*; D011189:Potassium Chloride / Q000493:pharmacokinetics; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D012417:Rumen / Q000166:cytology*; D012756:Sheep; D012965:Sodium Chloride / Q000493:pharmacokinetics; D014617:Vacuoles / Q000201:enzymology
chemical_list: D000838:Anions; D001639:Bicarbonates; D002087:Butyrates; D002712:Chlorides; D002245:Carbon Dioxide; D012965:Sodium Chloride; D011189:Potassium Chloride; D006180:Proton-Translocating ATPases; D008274:Magnesium