title: Liquid chromatography with on-line electrochemical derivatization and fluorescence detection for the determination of phenols.
abstract: A new methodological approach for the determination of monosubstituted phenols is described. After liquid chromatographic separation of the analytes, an on-line electrochemical derivatization is carried out and the reaction products are detected fluorometrically. Phenols are oxidized in the electrochemical cell to form fluorescent dimers and higher oligomers, which were identified by on-line electrochemistry/mass spectrometry. Major advantages of the proposed method include enhanced selectivity and sensitivity. Without prior enrichment of the analytes, limits of detection down to 2 x 10(-9) M (20 fmol) may be reached for selected phenols, e.g., for 4-octylphenol, 4-ethylphenol, and 4-(1-indanyl)phenol. Only readily available instrumentation is required for these measurements.
authors.affiliation: Department of Chemical Analysis and MESA+ Research Institute, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
authors.forename: Jörg
authors.initials: J
authors.lastname: Meyer
authors.forename: André
authors.initials: A
authors.lastname: Liesener
authors.forename: Sebastian
authors.initials: S
authors.lastname: Götz
authors.forename: Heiko
authors.initials: H
authors.lastname: Hayen
authors.forename: Uwe
authors.initials: U
authors.lastname: Karst