Asymmetric Fos labeling in lobule X of the cerebellum following transtympanic tetrodotoxin (TTX) in the rat.
Unilateral transtympanic administration of the sodium channel blocker tetrodotoxin (75 microl of 3 mM TTX in 0.1 M citrate buffer, pH 5.0) produced behavioral symptoms indicative of unilateral vestibular disruption. Following survival times of 2 and 24 h immunocytochemistry for Fos revealed asymmetric labeling in the granular and molecular layers of lobule X of the cerebellum and in the medial cerebellar nucleus. The granular and molecular layers as well as the medial cerebellar nucleus ipsilateral to TTX treatment contained elevated levels of Fos relative to the same regions contralaterally and when compared to controls receiving equal volume injections of vehicle. The asymmetric changes in neuronal activity as measured by Fos (genetic activity) in lobule X implicate this region of the vestibulocerebellum in recovery from transient vestibular disruption in the intact system.
Saxon
Dale W
DW
Department of Anatomy and Cell Biology, Indiana University School of Medicine, Evansville Center for Medical Education, 8600 University Boulevard, Evansville, IN 47712, USA. dsaxon@iupui.edu
D000818:Animals; D002529:Cerebellar Nuclei / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism*; D007150:Immunohistochemistry; D007267:Injections; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D026941:Sodium Channel Blockers / Q000008:administration & dosage / Q000494:pharmacology*; D013779:Tetrodotoxin / Q000008:administration & dosage / Q000494:pharmacology*; D014432:Tympanic Membrane
D016760:Proto-Oncogene Proteins c-fos; D026941:Sodium Channel Blockers; D013779:Tetrodotoxin