title: Flt3-ligand induces adhesion of haematopoietic progenitor cells via a very late antigen (VLA)-4- and VLA-5-dependent mechanism.
abstract: The adhesion of haematopoietic progenitor cells (HPC) to the bone marrow microenvironment is a process regulated by cytokines. In this study, we have shown that flt3-ligand (FL), a growth factor that controls early haematopoiesis, regulated the function and expression of the beta-1 integrins, very late antigen (VLA)-4 and VLA-5 on HPC. The modulation of the adhesiveness of HPC by FL was studied by adhesion assays on umbilical vein endothelial cells (HUVEC). Stimulation by FL induced two peaks of increased adhesiveness of HPC. The first peak was at around 30 min and was mechanistically related to an activation of the beta-1 integrins, mainly VLA-4 and VLA-5. The second peak was at around 12 h and was related to increased expression of VLA-4 and VLA-5. The control of HPC adhesiveness by FL is a previously unreported property of FL that may be important for the homing and the retention of flt3-expressing HPC within the bone marrow microenvironment.
authors.affiliation: UMR 5540, Université Victor Ségalen Bordeaux 2, Bordeaux, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Solanilla
authors.forename: Christophe
authors.initials: C
authors.lastname: Grosset
authors.forename: Pascale
authors.initials: P
authors.lastname: Duchez
authors.forename: Patrick
authors.initials: P
authors.lastname: Legembre
authors.forename: Vincent
authors.initials: V
authors.lastname: Pitard
authors.forename: Maryse
authors.initials: M
authors.lastname: Dupouy
authors.forename: Françis
authors.initials: F
authors.lastname: Belloc
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Viallard
authors.forename: Josy
authors.initials: J
authors.lastname: Reiffers
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Boiron
authors.forename: Laure
authors.initials: L
authors.lastname: Coulombel
authors.forename: Jean
authors.initials: J
authors.lastname: Ripoche
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D004730:Endothelium, Vascular; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D039041:Integrin alpha4beta1 / Q000378:metabolism*; D039081:Integrin alpha5beta1 / Q000378:metabolism*; D008565:Membrane Proteins / Q000502:physiology*; D014471:Umbilical Veins
chemical_list: D018952:Antigens, CD34; D039041:Integrin alpha4beta1; D039081:Integrin alpha5beta1; D008565:Membrane Proteins; C084881:flt3 ligand protein