title: R-factor in Proteus vulgaris from ulcerative disease of fish, Channa punctatus.
abstract: A Proteus vulgaris isolated from external ulcers of the fresh water fish Channa punctatus showed multidrug resistance and heavy metal tolerance. The isolate from the ulcer showed resistance to chloramphenicol (Ch), nalidixic acid (Nx), streptomycin (Str) and tetracycline (Tet) with minimum inhibitory concentration (MIC) values of 750, 150, 75 and 125 microg/ml, respectively. The isolate showed growth in medium containing cadmium (Cd2+), up to a concentration of 2.5 mM indicating its heavy metal tolerance. Resistance to Ch, Str, Tet and Cd2+ of the isolate was lost after plasmid curing. Presence of plasmid DNA in the wild type and its absence in the cured P. vulgaris suggested that the resistance were plasmid mediated.
authors.affiliation: Department of Bacteriology and Serology, Calcutta School of Tropical Medicine, C. R. Avenue, Kolkata 700 073, India.
authors.forename: S
authors.initials: S
authors.lastname: Mandal
authors.forename: M
authors.initials: M
authors.lastname: Mandal
authors.forename: N K
authors.initials: NK
authors.lastname: Pal
authors.forename: P K
authors.initials: PK
authors.lastname: Halder
authors.forename: P S
authors.initials: PS
authors.lastname: Basu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001424:Bacterial Infections / Q000382:microbiology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D005393:Fish Diseases / Q000382:microbiology*; D019216:Metals, Heavy / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011514:Proteus vulgaris / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D011815:R Factors; D014456:Ulcer / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D019216:Metals, Heavy