Infection of rats with Taenia taeniformis metacestodes increases hepatic CYP450, induces the activity of CYP1A1, CYP2B1 and COH isoforms and increases the genotoxicity of the procarcinogens benzo[a]pyrene, cyclophosphamide and aflatoxin B(1).
Infection of rat liver by Taenia taeniformis metacestodes produced an increase in total CYP450 content and induced activity of the CYP1A1, CYP2B1 and COH isoforms. Variations in activity and p450 total content were found with increasing time of infection. During increased activity of p450 isoforms, rats were challenged with carcinogens metabolized by the mentioned isozymes and an increased amount of genotoxic damage was found when benzo[a] pyrene, cyclophosphamide and aflatoxin B(1) were used. No change was seen in CYP2E1 activity. These results support previous findings regarding an increased susceptibility to genotoxic damage of infected organisms.
Montero
Regina
R
Instituto de Investigaciones Biomédicas and Facultad de Medicina, UNAM, Apdo Postal 70228, CP 04510, México DF, Mexico. dorinda@servidor.unam.mx
Serrano
Luis
L
Dávila
Víctor M
VM
Ito
Akira
A
Plancarte
Agustín
A
D016604:Aflatoxin B1; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D001564:Benzo(a)pyrene; D002273:Carcinogens; D002415:Cats; D003520:Cyclophosphamide; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D065637:Cytochrome P-450 CYP2A6; D019362:Cytochrome P-450 CYP2B1 / Q000378:metabolism*; D005260:Female; D015151:Immunoblotting; D008099:Liver / Q000469:parasitology*; D008862:Microsomes, Liver / Q000187:drug effects; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D009153:Mutagens; D009596:Nitrophenols; D020033:Protein Isoforms; D051381:Rats; D017207:Rats, Sprague-Dawley; D012156:Reticulocytes / Q000378:metabolism; D013621:Taenia / Q000378:metabolism*; D013997:Time Factors
D002273:Carcinogens; D009153:Mutagens; D009596:Nitrophenols; D020033:Protein Isoforms; D001564:Benzo(a)pyrene; D003520:Cyclophosphamide; D016604:Aflatoxin B1; D006899:Mixed Function Oxygenases; D001189:Aryl Hydrocarbon Hydroxylases; D019363:Cytochrome P-450 CYP1A1; D065637:Cytochrome P-450 CYP2A6; D019362:Cytochrome P-450 CYP2B1; C024836:4-nitrophenol