[Influence of atrazine on the tyrosine environment of catalase].
By the UV difference spectrum and fluorescence spectrum technique, the influence of atrazine on tyrosine environment of catalase was studied. According to the results of the UV difference spectrum curve, the UV difference adsorption delta A260nm showed changing rapidly when pH ranging from 9.1 to 9.9. UV difference spectrum and fluorescence spectrum of atrazine-catalase were red shift that maybe caused by effect of hydrogen bonding. The fluorescence spectrum was sensitive to SDS and quenchable by KI. The results indicated that the tyrosine residues of catalase reside in hydrophilic core, and there is hydrogen bonding between atrazine and catalase probably. Atrazine has little effect on tyrosine environment of catalase.
Liu
Weiping
W
Institute of Environmental Science, Zhejiang University, Hangzhou 310027, China. wpliu@mail.hz.zj.cn
Yang
Weichun
W
Liu
Huijun
H
Chen
Xueping
X
D001280:Atrazine / Q000494:pharmacology*; D002374:Catalase / Q000737:chemistry / Q000187:drug effects*; D006540:Herbicides / Q000494:pharmacology*; D014443:Tyrosine / Q000737:chemistry*
D006540:Herbicides; D014443:Tyrosine; D002374:Catalase; D001280:Atrazine