A simple, high-yield method for obtaining multipotential mesenchymal progenitor cells from trabecular bone.
In vitro cultures of primary, human trabecular bone-derived cells represent a useful system for investigation of the biology of osteoblasts. Our recent discovery of the multilineage mesenchymal differentiation potential of trabecular bone-derived cells suggests the potential application of these cells as mesenchymal progenitors for tissue repair and regeneration. Such applications are crucially dependent on efficient cell-isolation protocols to yield cells that optimally proliferate and differentiate. In this study, we describe a simple, high-yield procedure, requiring minimal culture expansion, for the isolation of mesenchymal progenitor cells from human trabecular bone. Moreover, these cells retain their ability to differentiate along multiple mesenchymal lineages through successive subculturing. Cell populations isolated and cultured as described here allow the efficient acquisition of a clinically significant number of cells, which may be used as the cell source for tissue-engineering applications.
Tuli
Richard
R
Cartilage Biology and Orthopedics Branch, 50 South Drive, Rm 1503, MSC 8022, National Institute of Arthritis, and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Seghatoleslami
M Reza
MR
Tuli
Suraj
S
Wang
Mark L
ML
Hozack
William J
WJ
Manner
Paul A
PA
Danielson
Keith G
KG
Tuan
Rocky S
RS
D000368:Aged; D018786:Bone Substitutes; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D005270:Femur Head / Q000166:cytology*; D015870:Gene Expression; D006801:Humans; D008297:Male; D008648:Mesoderm / Q000166:cytology / Q000502:physiology; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D011786:Quality Control; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods
D018786:Bone Substitutes