title: Somatic embryogenesis and plant regeneration from immature embryo explant of papaya (Carica papaya L. cv. washington and honey dew).
abstract: Immature zygotic embryo explants of Carica papaya were cultured on MS medium supplemented with 2,4-D (2.0 mg/l) and formed globular embryos on explants without callus formation in 4-6 weeks. Maturation and conversion of somatic embryos was also achieved on the same medium. Cotyledonary stage embryos germinated to 63.66 and 68.33% in cv. honey dew and washington respectively in MS basal medium supplemented ABA (0.5 microm/l). Robust development and proliferation of plantlet roots in vitro was obtained on MS basal medium. Hardened plantlets have 60% survival rate.
authors.affiliation: Plant Tissue Culture Division, National Chemical Laboratory, Pune 411 008, India.
authors.forename: J
authors.initials: J
authors.lastname: Bhattacharya
authors.forename: S S
authors.initials: SS
authors.lastname: Khuspe
authors.forename: N N
authors.initials: NN
authors.lastname: Renukdas
authors.forename: S K
authors.initials: SK
authors.lastname: Rawal
mesh_terms: D029441:Carica / Q000196:embryology* / Q000254:growth & development; D003470:Culture Media; D012038:Regeneration; D012639:Seeds / Q000254:growth & development*
chemical_list: D003470:Culture Media