TGFbeta-induced factor: a candidate gene for high myopia.
OBJECTIVE
To investigate the coding exons of transforming growth factor (TGF)-beta-induced factor (TGIF) for mutations in Chinese patients with high myopia.


METHODS
Seventy-one individuals with high myopia of -6.00 D or less and 105 control subjects were screened by DNA sequencing for sequence alterations. Univariate analysis and logistic regression were performed to identify single-nucleotide polymorphisms (SNPs) and their interactions in TGIF that may be associated with myopia.


RESULTS
Six SNPs showed a significant difference (P < 0.05) between patient and control subject in univariate analysis. Four of them cause codon changes: G223R, G231S, P241T, and A262G. Among all the SNPs that entered multivariate analysis, only 657(T-->G) showed statistical significance in the logistic regression model (odds ratio 0.133; 95% confidence interval 0.037-0.488; P = 0.002).


CONCLUSIONS
TGIF is a probable candidate gene for high myopia. Further studies are needed to identify the underlying mechanism.
Lam
Dennis Shun Chiu
DS
Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Kowloon, Hong Kong.
Lee
Wing Shan
WS
Leung
Yuk Fai
YF
Tam
Pancy Oi Sin
PO
Fan
Dorothy S P
DS
Fan
Bao Jian
BJ
Pang
Chi Pui
CP
D000328:Adult; D044466:Asian People / Q000235:genetics; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D005091:Exons; D005260:Female; D005801:Genes, Homeobox; D018398:Homeodomain Proteins / Q000235:genetics*; D006723:Hong Kong / Q000453:epidemiology; D006801:Humans; D008297:Male; D009154:Mutation; D009216:Myopia / Q000208:ethnology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012097:Repressor Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D016212:Transforming Growth Factor beta / Q000235:genetics*
D018398:Homeodomain Proteins; D012097:Repressor Proteins; C097504:TGIF1 protein, human; D016212:Transforming Growth Factor beta