Synthesis of 6'-sulfodisaccharides by beta-N-acetylhexosaminidase-catalyzed transglycosylation.
Presulfated N-acetylglucosaminyl donor (pNP beta-D-6-SO3-GlcNAc) was applied for the synthesis of sulfosugars using the beta-N-acetylhexosaminidase-catalyzed transglycosylation, to afford the critically stereocontrolled sulfodisaccharides carrying the 6-sulfo GlcNAc residue at the non-reducing sides in one step.
Uzawa
Hirotaka
H
Laboratory of Advanced Bioelectronics, National Institute of Advanced Industrial Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba 305-8565, Japan. h.uzawa@aist.go.jp
Zeng
Xiaoxiong
X
Minoura
Norihiko
N
D001230:Aspergillus / Q000201:enzymology; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D006031:Glycosylation; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D013463:Sulfuric Acid Esters / Q000737:chemistry; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism*
D004187:Disaccharides; D013463:Sulfuric Acid Esters; D001619:beta-N-Acetylhexosaminidases