Two forms of RNA polymerase II holoenzyme display different abundance during the cell cycle.
We analyzed the composition and abundance of two forms of RNA polymerase II (pol II) holoenzyme in synchronized HeLa cells. We did not detect significant changes in pol II holoenzyme composition, but we noticed differences in the abundance of the two complexes at different stages of the cell cycle. Summarized data from several independent experiments demonstrate that pol II holoenzyme, which is purified by GST-TFIIS affinity chromatography, is more abundant during G1/S and S phases. Another form of pol II holoenzyme, which is purified by anti-CDK7 antibodies, shows relatively higher amounts in G2/M and early G1 phases.
Holland
Linda
L
Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario, Canada N1G 2W1. lholland@uoguelph.ca
Yankulov
Krassimir
K
D015153:Blotting, Western; D002453:Cell Cycle; D002469:Cell Separation; D002845:Chromatography; D002846:Chromatography, Affinity; D018844:Cyclin-Dependent Kinases / Q000737:chemistry / Q000276:immunology; D005434:Flow Cytometry; D016193:G1 Phase; D016195:G2 Phase; D005982:Glutathione Transferase / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D012319:RNA Polymerase II / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016196:S Phase; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D000095043:Cyclin-Dependent Kinase-Activating Kinase
D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase; D018844:Cyclin-Dependent Kinases; D012319:RNA Polymerase II; D000095043:Cyclin-Dependent Kinase-Activating Kinase