[Expression of EIIIA-fibronectin in injured rat skin used in estimation of wound interval].
OBJECTIVE
To observe the means of fibronectin(FN) alternative splicing and the expression of EIIIA-FN variant in rat skin after bruise, for the sake of providing some help for forensic estimation of wound interval.


METHODS
Total RNA was isolated from wounded skin, and reverse transcription polymerase chain reaction was conducted to amplify target segments.


RESULTS
Detectable EIIIA+(526 bp) segments, lacked in normal organize, was amplified at 1 h after experimental wound, and the levels were increased within 24 h.


CONCLUSIONS
The alternative splicing EIIIA-fibronectin variant would be a satisfied criterion for research of skin injury.
Liu
Ning-guo
NG
Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063. liuningguo@163.net
Chen
Yi-jiu
YJ
Huang
Xiao-hua
XH
D017398:Alternative Splicing; D000818:Animals; D004848:Epithelium / Q000293:injuries / Q000378:metabolism; D005353:Fibronectins / Q000096:biosynthesis* / Q000235:genetics; D005554:Forensic Medicine; D039041:Integrin alpha4beta1 / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000293:injuries / Q000378:metabolism*; D013997:Time Factors
D005353:Fibronectins; D039041:Integrin alpha4beta1; D012333:RNA, Messenger