Effect of female sex steroids on human endometrial CD16neg CD56bright natural killer cells.
OBJECTIVE
To clarify whether female sex steroids directly affect the bioactivity of the human endometrial CD16neg CD56bright natural killer (NK) cells.


METHODS
In vitro study.


METHODS
University obstetrics and gynecology department.


METHODS
Thirteen women with histologically normal endometrium who were undergoing hysterectomy and seven women during the first trimester of pregnancy who were undergoing selective termination.


METHODS
Endometrium or decidua was obtained.


METHODS
The effects of 17beta-estradiol or progesterone (10(-6), 10(-7), and 10(-8) M) on the proliferation, cytolytic activity, and cytokine secretion of the isolated endometrial CD16neg CD56bright NK cells were examined using a 3H-thymidine incorporation assay, 51Cr-releasing assay, and enzyme-linked immunosorbent assay, respectively.


RESULTS
Neither 17beta-estradiol nor progesterone had significant effects on the proliferation, cytolytic activity, and cytokine secretion of endometrial CD16neg CD56bright NK cells.


CONCLUSIONS
Female sex steroids do not directly affect the bioactivity of the human endometrial CD16neg CD56bright NK cells.
Kitaya
Kotaro
K
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto, Japan. kitaya@koto.kpu-m.ac.jp
Yasuda
Jinsuke
J
Nakayama
Takeshi
T
Fushiki
Shinji
S
Honjo
Hideo
H
D000328:Adult; D019002:CD56 Antigen / Q000096:biosynthesis* / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000502:physiology; D003656:Decidua / Q000276:immunology / Q000378:metabolism; D004717:Endometrium / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D004958:Estradiol / Q000276:immunology / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D011247:Pregnancy; D011374:Progesterone / Q000276:immunology / Q000494:pharmacology*; D017452:Receptors, IgG / Q000096:biosynthesis* / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D019002:CD56 Antigen; D017452:Receptors, IgG; D014409:Tumor Necrosis Factor-alpha; D011374:Progesterone; D004958:Estradiol; D007371:Interferon-gamma; D013936:Thymidine