title: ELISA and HPLC determination of the occurrence of aflatoxin M(1) in raw cow's milk.
abstract: Raw cow's milk collected from dairy farms in the province of Leon, Spain, was examined for aflatoxin M(1) (AFM(1)). The samples were analysed with a commercial competitive enzyme-linked immunosorbent assay (ELISA) kit and high-performance liquid chromatography (HPLC). The concentrations of AFM(1) in the milk extracts were initially estimated by ELISA, with recovery rates of 74.6-109% for artificially contaminated milk at levels of 10-80 ng x l(1). Samples found to contain more than 10 ng x l(1) were further quantified with HPLC. The mean recovery for this method was 89.3%. The quantification limit was 10 ng x l(1) for both ELISA and HPLC. Although AFM(1) was confirmed in only 3.3% of the samples, the concentrations in all these cases were lower than the maximum limit applicable to these products pursuant to European Union legislation. Both methods were validated with reference material certified by the Community Bureau of Reference.
authors.affiliation: Instituto de Toxicología de Castilla y León (INTOXCAL), Auda Real, d Parque cientfico de León 24006 León, Spain. intoxcal@infonegocio.com
authors.forename: M L
authors.initials: ML
authors.lastname: Rodríguez Velasco
authors.forename: M M
authors.initials: MM
authors.lastname: Calonge Delso
authors.forename: D
authors.initials: D
authors.lastname: Ordónez Escudero
mesh_terms: D016607:Aflatoxin M1 / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D013030:Spain
chemical_list: D016607:Aflatoxin M1