Use of the quorum-sensing inhibitor RNAIII-inhibiting peptide to prevent biofilm formation in vivo by drug-resistant Staphylococcus epidermidis.
Staphylococcus epidermidis is a frequent cause of infections associated with foreign bodies and indwelling medical devices. The bacteria are capable of surviving antibiotic treatment through encapsulation into biofilms. RNAIII-inhibiting peptide (RIP) is a heptapeptide that inhibits S. aureus pathogenesis by disrupting quorum-sensing mechanisms. In this study, RIP inhibited drug-resistant S. epidermidis biofilm formation through a mechanism similar to that evidenced for S. aureus. RIP is synergistic with antibiotics in eliminating 100% of graft-associated in vivo S. epidermidis infections, which suggests that RIP may be used to coat medical devices to prevent staphylococcal infections. Disruption of cell-cell communication can prevent infections associated with antibiotic-resistant strains.
Balaban
Naomi
N
Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel. nbalaban@ucdavis.edu
Giacometti
Andrea
A
Cirioni
Oscar
O
Gov
Yael
Y
Ghiselli
Roberto
R
Mocchegiani
Federico
F
Viticchi
Claudio
C
Del Prete
Maria Simona
MS
Saba
Vittorio
V
Scalise
Giorgio
G
Dell'Acqua
Giorgio
G
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion; D001426:Bacterial Proteins; D018441:Biofilms / Q000254:growth & development; D002460:Cell Line; D004351:Drug Resistance; D006801:Humans; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D013203:Staphylococcal Infections / Q000188:drug therapy; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D009842:Oligopeptides; D010750:Phosphoproteins; C111711:RNAIII inhibiting peptide; C428446:TRAP protein, Staphylococcus