Ralstonia basilensis M91-3, a denitrifying soil bacterium capable of using s-triazines as nitrogen sources.
The purpose of this study was to characterize the phylogenetic and phenotypic traits of M91-3, a soil bacterium capable of mineralizing atrazine (2-chloro-4-N-isopropyl-6-N-ethyl-s-triazine). The isolate was identified as Ralstonia basilensis based on 99.5% homology of the 16S rRNA sequence and various chemotaxonomic data. The isolate used atrazine as the sole source of energy, carbon, and nitrogen. It could also use several other s-triazines as nitrogen sources. Ralstonia basilensis M91-3 was capable of denitrification, which was confirmed by gas chromatographic analysis of nitrous oxide under acetylene blockage conditions.
Stamper
David M
DM
Department of Microbiology, Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA.
Radosevich
Mark
M
Hallberg
Kevin B
KB
Traina
Samuel J
SJ
Tuovinen
Olli H
OH
D001483:Base Sequence; D020562:Betaproteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016208:Databases, Factual; D005227:Fatty Acids / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D014227:Triazines / Q000378:metabolism*
D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014227:Triazines; D009584:Nitrogen