[The gene expression of nuclear transcriptional factor-kappa B and I kappa B in autogenous vein graft in rats].
OBJECTIVE
To investigate the gene expression of nuclear transcriptional factor-kappa B (NF-kappa B) p65 and its inhibiting factor I kappa B in autogenous vein graft.


METHODS
The right common jugular vein was transplanted to infrarenal abdominal aorta by microsurgical technique among 80 Wistar rats so as to establish an autogenous vein graft model. Ten vein graft samples were harvested 6 hours, 24 hours, 3 days, 7 days, 2 weeks, 4 weeks, 6 weeks, and 8 weeks after surgery. NF kappa B p65 mRNA and I kappa B beta mRNA were measured by reverse transcription-PCR and in situ hybridization. Western blotting and immunohistochemistry were used to detect the protein expression of NF kappa B p65 and I kappa B.


RESULTS
The expressions of NF kappa B p65 mRNA and I kappa B beta mRNA 6 hours after the surgery were 16% +/- 4% and 31% +/- 9% respectively (P < 0.01 vs. control vein). The expression of NF kappa B p65 mRNA reached in peak during the period 3 days to 7 days after the surgery (37% +/- 12% and 34% +/- 10% respectively, P< 0.01 vs. other teams), however, the I kappa B beta mRNA expression reached its peak during 1 to 2 weeks after the surgery (53% +/- 17% and 49% +/- 10% respectively, P < 0.01 vs. other teams). The expressions of NF kappa B p65 mRNA and I kappa B beta mRNA recovered to their baseline values 6 weeks after surgery. The expression of p65 protein reached its peak 1 week after the surgery (32% +/- 13%) and then decreased gradually. The expressions of I kappa B alpha and I kappa B beta decreased to 1/3 to 1/2 of the normal vein 6 hours to 24 hours after the surgery and then increased to 5 times that of the control vein 2 weeks after surgery (35% +/- 11% and 44% +/- 13% respectively).


CONCLUSIONS
The NF-kappa B/I kappa B system is activated in autogenous vein graft. The NF kappa B may become a new target for the prevention and therapy of intimal hyperplasia and stenosis after vein graft.
Hu
Xinhua
X
Department of Vascular Surgery, First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Zhang
Qiang
Q
Sun
Daxin
D
Duan
Peiyuan
P
Wang
Xinwen
X
Duan
Zhiquan
Z
D000818:Animals; D001012:Aorta, Abdominal / Q000601:surgery; D015153:Blotting, Western; D005260:Female; D015870:Gene Expression; D020672:I-kappa B Proteins / Q000235:genetics* / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007601:Jugular Veins / Q000378:metabolism / Q000637:transplantation*; D008297:Male; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014182:Transplantation, Autologous
D020672:I-kappa B Proteins; D016328:NF-kappa B; D012333:RNA, Messenger