title: Complete mitochondrial DNA sequence of the important honey bee pest, Varroa destructor (Acari: Varroidae).
abstract: Mites in the genus Varroa are the primary parasites of honey bees on several continents. Genetic analyses based on Varroa mitochondrial DNA have played a central role in establishing Varroa taxonomy and dispersal. Here we present the complete mitochondrial sequence of the important honey bee pest Varroa destructor. This species has a relatively compact mitochondrial genome (15,218 bp). The order of genes encoding proteins is identical to that of most arthropods. Ten of 22 transfer RNAs are in different locations relative to hard ticks, and the 12S ribosomal RNA subunit is inverted and separated from the 16S rRNA by a novel non-coding region, a trait not yet seen in other arthropods. We describe a dispersed set of 45 oligonucleotide primers that can be used to address genetic questions in Varroa. A subset of these primers should be useful for taxonomic and phylogenetic studies in other mites and ticks.
authors.affiliation: USDA-ARS Bee Research Lab, BARC-East Bldg. 476, Beltsville, MD 20705, USA. evansj@ba.ars.usda.gov
authors.forename: Jay D
authors.initials: JD
authors.lastname: Evans
authors.forename: Dawn L
authors.initials: DL
authors.lastname: Lopez
mesh_terms: D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000469:parasitology*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D014644:Genetic Variation; D008925:Mites / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; D012343:RNA, Transfer