New aspects of the simultaneous analysis of amino acids and amines as their o-phthaldialdehyde derivatives by high-performance liquid chromatography. Analysis of wine, beer and vinegar.
A new high-performance liquid chromatography method is described for the simultaneous quantitation of amino acids and amines for 37 compounds (20 amino acids + 17 amines), as their o-phthaldialdehyde (OPA)-3-mercaptopropionic acid derivatives, within 53 min. Based on previously documented stoichiometric and reaction mechanism studies, derivatizations have been carried out with the OPA-SH-group = 1:50 containing reagents. Reliability and reproducibility of analyses have been considerably improved. Average reproducibility data in a wide concentration range of derivatives had RSD < or = 3.4%.
Kutlán
D
D
Institute of Inorganic and Analytical Chemistry, L. Eötvös University, P.O. Box 32, H-1518 Budapest 112, Hungary.
Molnár-Perl
I
I
D019342:Acetic Acid / Q000032:analysis*; D000588:Amines / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis*; D001515:Beer / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007202:Indicators and Reagents / Q000737:chemistry*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D014920:Wine / Q000032:analysis*; D009764:o-Phthalaldehyde / Q000737:chemistry*
D000588:Amines; D000596:Amino Acids; D007202:Indicators and Reagents; D009764:o-Phthalaldehyde; D019342:Acetic Acid