title: Simple method for the quantitative examination of extra column band broadening in microchromatographic systems.
abstract: In recent years capillary chromatography has gained popularity for trace analyses. Most often UV or electrochemical detection is employed because the small peak volumes make post-column derivatization challenging. We have developed a simple method based on flow injection for determining contributions to peak broadening from post-column reactors. The only requirement for application of our methodology is that diffusion be in the Taylor regime so that radial concentration gradients are relaxed enabling mixing purely by diffusion.
authors.affiliation: Department of Chemistry, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, PA 15260, USA.
authors.forename: Amy T
authors.initials: AT
authors.lastname: Beisler
authors.forename: Kathleen E
authors.initials: KE
authors.lastname: Schaefer
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Weber
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet