Distribution of Aeromonas hydrophila serogroups in different clinical samples and the development of polyclonal antibodies for rapid identification of the genus Aeromonas by direct agglutination.
We characterized a collection of 256 Aeromonas hydrophila strains isolated from blood, discharge and stool for their serogroup designation. Of these, 2.3% were untypable and 15.2% were rough strains. Among the typable strains, about 50% comprised serogroups O:11, O:16, O:18, O:34 and O:83. To develop rapid differentiation of Aeromonas from other oxidase-positive bacteria, antisera against Aeromonas were produced to establish a direct, genus-specific, agglutination test. It was found that among 105 isolates of Aeromonas, 102 showed positive results with the agglutination test. The calculated sensitivity and specificity were 97.1% and 90.7%, respectively.
Korbsrisate
Sunee
S
Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. grsks@mahidol.ac.th
Dumnin
Suwat
S
Chawengkirttikul
Rungrawan
R
Gherunpong
Vanchit
V
Eampokalap
Boonchuay
B
Gongviseisoog
Charles
C
Janyapoon
Kritsana
K
Lertpocasombat
Kancharee
K
Shimada
Toshio
T
D000333:Aeromonas / Q000276:immunology / Q000302:isolation & purification*; D016980:Aeromonas hydrophila / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D000372:Agglutination Tests / Q000379:methods*; D000907:Antibodies, Bacterial / Q000097:blood*; D015153:Blotting, Western / Q000379:methods; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis*; D006801:Humans; D019081:O Antigens / Q000032:analysis; D012703:Serotyping
D000907:Antibodies, Bacterial; D019081:O Antigens