The percentage of CD31+ T cells decreases after open but not laparoscopic surgery.
BACKGROUND
Efficient killing of tumor cells depends on T cells that migrate from the circulation to the peripheral tissues; these cells express CD31. This study was undertaken to determine the impact of open (OS) and laparoscopic (LS) colorectal surgery on the percentage of circulating CD3+CD31+ cells.


METHODS
Peripheral blood was collected from 27 OS and 24 LS colon cancer patients preoperatively (preOP) and on postoperative days 1 (POD1) and 3 (POD3). CD31+ T cells were assessed by flow cytometry using monoclonal antibodies.


RESULTS
In the OS group, the percentage of CD3+CD31+ cells was significantly lower in POD1 and POD3 samples compared to the preOP results. LS surgery did not result in a significant change in the percentage of these T cells. A significant correlation was found between the decrease in the percentage of CD3+CD31+ cells and the length of incision in OS patients.


CONCLUSIONS
The percentage of CD3+CD31+ cells decreases following OS but not LS and may be related to incision length. This may compromise T cell function in the peripheral tissues in the postoperative period.
Kirman
I
I
Department of Surgery, College of Physicians and Surgeons of Columbia University, BB1716, 630 W. 168th Street, New York, NY 10032, USA. ik139@columbia.edu
Cekic
V
V
Poltaratskaia
N
N
Asi
Z
Z
Conte
S
S
Feingold
D
D
Forde
K A
KA
Huang
E H
EH
Whelan
R L
RL
D000293:Adolescent; D017252:CD3 Complex / Q000096:biosynthesis; D003106:Colon / Q000098:blood supply; D015179:Colorectal Neoplasms / Q000098:blood supply / Q000601:surgery; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D010535:Laparoscopy / Q000379:methods*; D018655:Lymphocyte Count / Q000379:methods; D008297:Male; D008833:Microcirculation; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis*; D012007:Rectum / Q000098:blood supply; D013601:T-Lymphocytes / Q000378:metabolism*
D017252:CD3 Complex; D019408:Platelet Endothelial Cell Adhesion Molecule-1