Determination of deoxynivalenol and nivalenol in corn and wheat by liquid chromatography with electrospray mass spectrometry.
The fungus Fusarium graminearum is a pathogen of both wheat and corn. Strains of the fungus from the United States produce a toxin, deoxynivalenol (DON); strains of the fungus from Asia and Europe produce DON or a related toxin, nivalenol. These toxins can cause disease in livestock, and their potential presence in feed and foods is a concern for animal and human health. A method was developed to detect both toxins in corn and wheat by liquid chromatography/mass spectrometry of an extract of ground grain. The method requires no sample cleanup and can detect the toxins at 0.05 microg/g.
Plattner
Ronald D
RD
U.S. Department of Agriculture, Mycotoxin Research, National Center for Agricultural Utilization Research, Midwest Area, Agriculture Research Service, Peoria, IL 61604, USA. plattnrd@ncaur.usda.gov
Maragos
Chris M
CM
D002853:Chromatography, Liquid / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry; D009183:Mycotoxins / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D014255:Trichothecenes / Q000032:analysis*; D014908:Triticum / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
D009183:Mycotoxins; D010936:Plant Extracts; D014255:Trichothecenes; C038405:nivalenol; C007262:deoxynivalenol