title: DsbA of Pseudomonas aeruginosa is essential for multiple virulence factors.
abstract: DsbA is a periplasmic thiol:disulfide oxidoreductase which contributes to the process of protein folding by catalyzing the formation of disulfide bonds. In this study, we demonstrate that the dsbA gene is required for the expression of the type III secretion system under low-calcium inducing conditions, intracellular survival of P. aeruginosa upon infection of HeLa cells, and twitching motility. The diverse phenotypes of the dsbA mutant are likely due to its defect in the folding of proteins that are involved in various biological processes, such as signal sensing, protein secretion, and defense against host clearing. In light of its effect on various virulence factors, DsbA could be an important target for the control of P. aeruginosa infections.
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida 32610, USA.
authors.forename: Un-Hwan
authors.initials: UH
authors.lastname: Ha
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.forename: Shouguang
authors.initials: S
authors.lastname: Jin
mesh_terms: D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D019704:Protein Disulfide-Isomerases / Q000737:chemistry / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity*; D037521:Virulence Factors / Q000502:physiology*
chemical_list: D037521:Virulence Factors; D019704:Protein Disulfide-Isomerases