title: Involvement of cytotoxic granules in the apoptosis of aplastic anaemia.
abstract: To study the involvement of cytotoxic granules in apoptosis of aplastic anaemia (AA), we analysed bone marrow clot sections of AA for apoptosis and perforin, granzyme B and T-cell intracellular antigen-1 (TIA-1) expression. Perforin, TIA-1 expression and apoptosis were significantly increased in AA patients compared with control subjects, but granzyme B was not. TIA-1 was significantly correlated with apoptotic cells of AA. No correlation was found between perforin and apoptotic cells of AA. These results suggest that perforin and TIA-1 play a role in the pathogenesis of AA. TIA-1 is implicated as an effector in the apoptosis of AA.
authors.affiliation: Department of Laboratory Medicine, Nagoya University School of Medicine, Nagoya, Japan.
authors.forename: J L
authors.initials: JL
authors.lastname: Xu
authors.forename: T
authors.initials: T
authors.lastname: Nagasaka
authors.forename: N
authors.initials: N
authors.lastname: Nakashima
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000378:metabolism / Q000473:pathology*; D017209:Apoptosis / Q000502:physiology; D001853:Bone Marrow; D002648:Child; D003594:Cytoplasmic Granules; D005260:Female; D053804:Granzymes; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D054353:Perforin; D039102:Poly(A)-Binding Proteins; D052899:Pore Forming Cytotoxic Proteins; D011506:Proteins; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism*; D000074019:T-Cell Intracellular Antigen-1
chemical_list: D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D039102:Poly(A)-Binding Proteins; D052899:Pore Forming Cytotoxic Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; D000074019:T-Cell Intracellular Antigen-1; C071558:TIA1 protein, human; D054353:Perforin; C509213:GZMB protein, human; D053804:Granzymes; D012697:Serine Endopeptidases