Expression of acetoacetyl-CoA synthetase, a novel cytosolic ketone body-utilizing enzyme, in human brain.
Acetoacetyl-CoA synthetase (AACS, acetoacetate-CoA ligase, EC 6.2.1.16) is a ketone body-utilizing enzyme, the physiological role of which remains unclear yet in mammals, particularly has never been studied in human. In order to investigate the tissue distribution of AACS in human, cDNA encoding AACS was isolated from HepG2 cells. Amino acid sequence of human AACS deduced from the open reading frame showed high homology (89.3%) with that of rat AACS and much less homology (43.7%) with that of bacterial AACS. The expression level of the AACS mRNA was high in kidney, heart and brain, but low in liver, and the expression profile of AACS in the human brain was quite similar to that of 3-hydroxy-3-methylglutaryl-CoA reductase.
Ohgami
Masahiro
M
Department of Health Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan.
Takahashi
Noriko
N
Yamasaki
Masahiro
M
Fukui
Tetsuya
T
D000090:Acetoacetates / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology* / Q000378:metabolism; D003066:Coenzyme A Ligases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis; D015870:Gene Expression; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism; D007657:Ketone Bodies / Q000378:metabolism*; D008969:Molecular Sequence Data; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D000090:Acetoacetates; D018076:DNA, Complementary; D007657:Ketone Bodies; C016635:acetoacetic acid; D006903:Hydroxymethylglutaryl CoA Reductases; D003066:Coenzyme A Ligases; C023747:acetoacetyl-CoA synthetase