title: Indirect 17(O)-magnetic resonance imaging of cerebral blood flow in the rat.
abstract: Proton T(1rho)-dispersion MRI is demonstrated for indirect, in vivo detection of (17)O in the brain. This technique, which may be readily implemented on any clinical MRI scanner, is applied towards high-resolution, quantitative mapping of cerebral blood flow (CBF) in the rat by monitoring the clearance of (17)O-enriched water. Strategies are derived and employed for 1) quantitation of absolute H(2) (17)O tracer concentration from a ratio of high- and low-frequency spin-locked T(1rho) images, and 2) mapping CBF without having to transform the T(1rho) signal to H(2) (17)O tracer concentration. Absolute regional blood flow was mapped in a single 3-mm brain slice at an in-plane resolution of 0.4 x 0.8 mm within a 5-min tracer washout time; these data are consistent with the less localized CBF measurements reported in the literature. T(1rho)-weighted imaging yields excellent signal-to-noise ratios, spatiotemporal resolution, and anatomical contrast for mapping CBF.
authors.affiliation: Metabolic Magnetic Resonance Research and Computing Center, Department of Radiology, University of Pennsylvania, Philadelphia 19104, USA. dtailor@mail.mmrrcc.upenn.edu
authors.forename: Dharmesh R
authors.initials: DR
authors.lastname: Tailor
authors.forename: Arijit
authors.initials: A
authors.lastname: Roy
authors.forename: Ravinder R
authors.initials: RR
authors.lastname: Regatte
authors.forename: Sridhar R
authors.initials: SR
authors.lastname: Charagundla
authors.forename: Alan C
authors.initials: AC
authors.lastname: McLaughlin
authors.forename: John S
authors.initials: JS
authors.lastname: Leigh
authors.forename: Ravinder
authors.initials: R
authors.lastname: Reddy
mesh_terms: D000818:Animals; D002560:Cerebrovascular Circulation; D005240:Feasibility Studies; D005260:Female; D008279:Magnetic Resonance Imaging / Q000379:methods*; D010103:Oxygen Isotopes; D051381:Rats; D017207:Rats, Sprague-Dawley; D014867:Water / Q000378:metabolism
chemical_list: D010103:Oxygen Isotopes; D014867:Water