title: Selenium supplementation can protect cultured rat cardiomyocytes from hypoxia/reoxygenation damage.
abstract: The possibility of enhancing glutathione peroxidase (GPx) activity and cytosolic total antioxidant activity (TAA) in normoxia and hypoxia/reoxygenation (H/R) by the supplementation of different concentrations of sodium selenite (SS) or selenomethionine (SM) was investigated in cultured rat cardiomyocytes. To assess the entity of oxidative stress due to H/R, levels of conjugated dienes containing lipids were determined. In normoxia, GPx activity and TAA increased in parallel with the increase in SS and SM supplementation. H/R did not influence GPx activity but lowered TAA; both SS and SM supplementations were effective in increasing GPx activity, the most effective concentration being 1 microM. At this SS and SM concentration, TAA returned to a normoxic value. Conjugated diene production, increased by H/R, was reduced by SS and SM supplementation, the 1 microM concentration appearing to be the most effective one. According to these data Se supplementation represents another possibility to counteract oxidative damage in the myocardium.
authors.affiliation: Nutrition Research Center, Department of Biochemistry G. Moruzzi, University of Bologna, via Irnerio 48, Italy. bordoni@biocfarm.unibo.it
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bordoni
authors.forename: Pier L
authors.initials: PL
authors.lastname: Biagi
authors.forename: Cristina
authors.initials: C
authors.lastname: Angeloni
authors.forename: Emanuela
authors.initials: E
authors.lastname: Leoncini
authors.forename: Ivan
authors.initials: I
authors.lastname: Muccinelli
authors.forename: Silvana
authors.initials: S
authors.lastname: Hrelia
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis; D015687:Cell Hypoxia / Q000187:drug effects*; D002478:Cells, Cultured; D005979:Glutathione Peroxidase / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D010100:Oxygen / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000008:administration & dosage*; D012645:Selenomethionine / Q000008:administration & dosage; D018038:Sodium Selenite / Q000008:administration & dosage
chemical_list: D000975:Antioxidants; D012645:Selenomethionine; D005979:Glutathione Peroxidase; D012643:Selenium; D018038:Sodium Selenite; D010100:Oxygen