Survival of biological cells deformed in a narrow gap.
Recent studies show that during slow freezing of biological cells, the cells may be also injured by not only chemical damage but also mechanical damage induced by ice crystal compression. A new experimental procedure is developed to quantify cell destruction by deformation with two parallel surfaces. The viability of cells (prostatic carcinoma cells, 17.5 microns in mean diameter) is measured as a function of gap size ranging from 3.5 microns to 16.2 microns at 0 degree C, 23 degrees C and 37 degrees C. The viability at a smaller gap size is significantly lower at 37 degrees C than at 23 degrees C, while the difference between 0 degree C and 23 degrees C is much smaller. This suggests that deformation damage is related to the deformation of the cytoskeleton rather than the mechanical properties of the lipid membrane.
Takamatsu
Hiroshi
H
Institute of Advanced Material Study, Kyushu University, 6-1 Kasugakoen, Kasuga 816-8580, Japan. takamatu@cm.kyushu-u.ac.jp
Kumagae
Norihiko
N
D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000473:pathology / Q000503:physiopathology; D048429:Cell Size; D002470:Cell Survival; D003080:Cold Temperature; D005615:Freezing; D006801:Humans; D007038:Hypotonic Solutions; D008297:Male; D009997:Osmotic Pressure; D011471:Prostatic Neoplasms / Q000473:pathology* / Q000503:physiopathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013223:Statistics as Topic; D013314:Stress, Mechanical; D013696:Temperature; D014407:Tumor Cells, Cultured
D007038:Hypotonic Solutions