In vivo time-lapse imaging of cell divisions during neurogenesis in the developing zebrafish retina.
Two-photon excitation microscopy was used to reconstruct cell divisions in living zebrafish embryonic retinas. Contrary to proposed models for vertebrate asymmetric divisions, no apico-basal cell divisions take place in the zebrafish retina during the generation of postmitotic neurons. However, a surprising shift in the orientation of cell division from central-peripheral to circumferential occurs within the plane of the ventricular surface. In the sonic you (syu) and lakritz (lak) mutants, the shift from central-peripheral to circumferential divisions is absent or delayed, correlating with the delay in neuronal differentiation and neurogenesis in these mutants. The reconstructions here show that mitotic cells always remain in contact with the opposite basal surface by means of a thin basal process that can be inherited asymmetrically.
Das
Tilak
T
Department of Anatomy, University of Cambridge, Cambridge CB2 3DY, United Kingdom.
Payer
Bernhard
B
Cayouette
Michel
M
Harris
William A
WA
D000818:Animals; D002455:Cell Division / Q000502:physiology; D005456:Fluorescent Dyes; D008164:Luminescent Proteins / Q000235:genetics; D018715:Microscopy, Video; D008938:Mitosis; D009154:Mutation; D009474:Neurons / Q000166:cytology*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012160:Retina / Q000166:cytology* / Q000196:embryology*; D013234:Stem Cells / Q000166:cytology; D013997:Time Factors; D015027:Zebrafish
D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins