title: Separation of peptides by pressurized capillary electrochromatography.
abstract: A pressurized electrochromatography (pCEC) instrument with gradient capability was used in this work for separation of peptides. Three separation modes, namely, pCEC, high-performance liquid chromatography and capillary electrophoresiscan be carried out with the instrument. In pCEC mode, the mobile phase is driven by both electroosmotic flow and pressurized flow, facilitating fine-tuning in selectivity of neutral and charged species. A continuous gradient elution can be carried out conveniently on this instrument, which demonstrates that it is more powerful than isocratic pCEC for separation of complicated samples. The effects of applied voltage, supplementary pressure and ion-pairing agents on separation of peptides in gradient pCEC were investigated. The effects of flow-rate of the pump and the volume of the mixer on resolution were also evaluated.
authors.affiliation: State Key Laboratory of Elemento-Organic Chemistry, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Zhengjin
authors.initials: Z
authors.lastname: Jiang
authors.forename: Chuanyi
authors.initials: C
authors.lastname: Yao
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Qinsun
authors.initials: Q
authors.lastname: Wang
authors.forename: Ruyu
authors.initials: R
authors.lastname: Gao
authors.forename: Chao
authors.initials: C
authors.lastname: Yan
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D010455:Peptides / Q000302:isolation & purification*; D011312:Pressure
chemical_list: D010455:Peptides