Determination of midodrine in human plasma by high-performance liquid chromatography with fluorescence detection.
A simple and sensitive fluorometric high-performance liquid chromatographic method was developed for the determination of midodrine in human plasma. After liquid-liquid extraction from plasma, the drug and 2-phenylglycinol (internal standard) were convened into the corresponding fluorescent derivatives by reaction with 3,4-dihydro-6,7-dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride, a fluorescence derivatization reagent for amines. The derivatives were separated within 30 min on a reversed-phase column using isocratic elution with acetonitrile-methanol-water (10:30:60, v/v) and were detected spectrofluorometrically at 485 nm with excitation at 400 nm. The detection limit for midodrine was 0.3 pmol (76 pg) per mL plasma at a signal-to-noise ratio of 3.
Yoshida
Hideyuki
H
Faculty of Pharmaceutical Sciences, Fukuoka University, Nanakuma, Johnan, Fukuoka 814-0180, Japan.
Ohno
Yuki
Y
Yoshikuni
Kenichiro
K
Todoroki
Kenichiro
K
Nohta
Hitoshi
H
Yamaguchi
Masatoshi
M
D000316:Adrenergic alpha-Agonists / Q000008:administration & dosage / Q000097:blood / Q000302:isolation & purification; D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D008297:Male; D008879:Midodrine / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D011810:Quinoxalines; D012015:Reference Standards; D012680:Sensitivity and Specificity
D000316:Adrenergic alpha-Agonists; D011810:Quinoxalines; C063399:3,4-dihydro-6,7-dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride; D008879:Midodrine