title: Elucidation of Nipah virus morphogenesis and replication using ultrastructural and molecular approaches.
abstract: Nipah virus, which was first recognized during an outbreak of encephalitis with high mortality in Peninsular Malaysia during 1998-1999, is most closely related to Hendra virus, another emergent paramyxovirus first recognized in Australia in 1994. We have studied the morphologic features of Nipah virus in infected Vero E6 cells and human brain by using standard and immunogold electron microscopy and ultrastructural in situ hybridization. Nipah virions are enveloped particles composed of a tangle of filamentous nucleocapsids and measured as large as 1900 nm in diameter. The nucleocapsids measured up to 1.67 microm in length and had the herringbone structure characteristic for paramyxoviruses. Cellular infection was associated with multinucleation, intracytoplasmic nucleocapsid inclusions (NCIs), and long cytoplasmic tubules. Previously undescribed for other members of the family Paramyxoviridae, infected cells also contained an inclusion formed of reticular structures. Ultrastructural ISH studies suggest these inclusions play an important role in the transcription process.
authors.affiliation: Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Mailstop G30, 1600 Clifton Road NE, Atlanta, GA 30333, USA. cgoldsmith@cdc.gov
authors.forename: Cynthia S
authors.initials: CS
authors.lastname: Goldsmith
authors.forename: Toni
authors.initials: T
authors.lastname: Whistler
authors.forename: Pierre E
authors.initials: PE
authors.lastname: Rollin
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Ksiazek
authors.forename: Paul A
authors.initials: PA
authors.lastname: Rota
authors.forename: William J
authors.initials: WJ
authors.lastname: Bellini
authors.forename: Peter
authors.initials: P
authors.lastname: Daszak
authors.forename: K T
authors.initials: KT
authors.lastname: Wong
authors.forename: Wun-Ju
authors.initials: WJ
authors.lastname: Shieh
authors.forename: Sherif R
authors.initials: SR
authors.lastname: Zaki
mesh_terms: D000818:Animals; D001921:Brain / Q000821:virology; D002522:Chlorocebus aethiops; D018792:Encephalitis, Viral / Q000821:virology; D006801:Humans; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D016253:Microscopy, Immunoelectron; D018184:Paramyxoviridae Infections / Q000821:virology; D018098:Paramyxovirinae / Q000235:genetics / Q000254:growth & development* / Q000502:physiology* / Q000648:ultrastructure; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D014709:Vero Cells; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D012367:RNA, Viral