Effects of enamel matrix protein application on the viability, proliferation, and attachment of human periodontal ligament fibroblasts to diseased root surfaces in vitro.
OBJECTIVE
The purpose of this research was to examine the influence of enamel matrix proteins (EMP) on the viability, proliferation, and attachment of periodontal ligament fibroblasts (PDLF) to diseased root surfaces.


METHODS
Primary cell cultures of PDFL were obtained from clinically healthy third molars or premolar teeth. Viability and proliferation rates were carried out over a 10-day period. A total of 80,000 cells were plated in 24-well plates followed by EMEM with 10% FBS (positive control) and EMEM plus EMP at 25, 50, 75, and 100 micro g/ml. Cells were harvested on days 1, 3, 5, 7, and 10 and viability was performed utilizing an MTS assay. PDLF proliferation rates were assessed by a CyQUANT GR dye assay. SEM analysis was used to examine the qualitative effects of cellular attachment to diseased root surfaces following EMP compared to nontreated controls.


RESULTS
The results indicated that viability was negatively affected for higher doses over time while lower doses displayed viability effects similar to control. Proliferation, however, appeared to be ameliorated following exposure to EMP. The SEM analysis suggests that cellular attachment to diseased dentin was enhanced following EMP application.


CONCLUSIONS
These in vitro studies support the concept that EMP may act as a suitable matrix for PDLF.
Davenport
D R
DR
Medical College of Georgia, Augusta, GA 30912, USA.
Mailhot
J M
JM
Wataha
J C
JC
Billman
M A
MA
Sharawy
M M
MM
Shrout
M K
MK
D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D002448:Cell Adhesion / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D003804:Dentin; D004305:Dose-Response Relationship, Drug; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D010510:Periodontal Diseases; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D012038:Regeneration / Q000187:drug effects; D014092:Tooth Root
D003746:Dental Enamel Proteins; C109069:enamel matrix proteins