Retinoic acid downregulates the expression of ciliary neurotrophic factor in rat Schwann cells.
Neuropoietic cytokines, which serve as mediators in neuroglial interactions, are differentially regulated after peripheral nerve injury. In Schwann cells, the expression of ciliary neurotrophic factor (CNTF) decreases. Pursuing the hypothesis that retinoic acid (RA) serves as a regulator of lesion-induced cytokine signaling we found that all RA receptors and retinoid X receptors are expressed in Schwann cell primary cultures. Using quantitative reverse transcription-polymerase chain reaction, we have investigated the effect of RA on the expression of CNTF in these cells. After treatment with 10 nM all-trans RA for 22 h the concentration of CNTF mRNA was reduced to 63% of the control level, reminiscent of the regulation after nerve injury in vivo. In addition to CNTF, the mRNAs of leukemia inhibitory factor, interleukin-6, ciliary neurotrophic factor receptor component alpha and gp130 were detected in the Schwann cells.
Johann
Verena
V
Institut für Biologie II, RWTH-Aachen, Kopernikusstrasse 16, 52074, Aachen, Germany.
Jeliaznik
Nina
N
Schrage
Kirsten
K
Mey
Jörg
J
D000818:Animals; D000831:Animals, Newborn; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics; D002478:Cells, Cultured; D020934:Ciliary Neurotrophic Factor / Q000096:biosynthesis*; D050822:Cytokine Receptor gp130; D015536:Down-Regulation; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics; D006131:Growth Inhibitors / Q000096:biosynthesis / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000096:biosynthesis / Q000235:genetics; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020801:Receptor, Ciliary Neurotrophic Factor / Q000096:biosynthesis / Q000235:genetics; D018168:Receptors, Retinoic Acid / Q000096:biosynthesis / Q000235:genetics; D047488:Retinoid X Receptors; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012583:Schwann Cells / Q000378:metabolism*; D012584:Sciatic Nerve / Q000166:cytology / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology / Q000502:physiology*
D015703:Antigens, CD; D020934:Ciliary Neurotrophic Factor; D006131:Growth Inhibitors; C494201:Il6st protein, rat; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D020801:Receptor, Ciliary Neurotrophic Factor; D018168:Receptors, Retinoic Acid; D047488:Retinoid X Receptors; D014157:Transcription Factors; D050822:Cytokine Receptor gp130; D014212:Tretinoin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases