Cloning and expression of a putative cyclodextrin glucosyltransferase from the symbiotically competent cyanobacterium Nostoc sp. PCC 9229.
A polymerase chain reaction-based method was used to isolate a Nostoc sp. PCC 9229 cDNA from infected glands of Gunnera chilensis. The complete gene sequence was isolated from a genomic Nostoc sp. PCC 9229 library. Sequence analysis showed 84% amino acid similarity to a putative cyclodextrin glycosyltransferase from Nostoc sp. PCC 7120 and the gene was therefore termed cgt. Southern blot revealed that the cgt gene was present in symbiotically competent cyanobacteria. The cgt gene was expressed in free-living nitrogen-fixing cultures in light or in darkness when supplemented with fructose. This is the first expression analysis of a cgt gene from a cyanobacterium.
Wouters
Johanna
J
Department of Biology and Environmental Science, Kalmar University, Barlastgatan 1, S-391 82, Kalmar, Sweden. johanna.wouters@hik.se
Bergman
Birgitta
B
Janson
Sven
S
D015152:Blotting, Northern; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D018076:DNA, Complementary; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005964:Glucosyltransferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism / Q000382:microbiology; D013559:Symbiosis
D018076:DNA, Complementary; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase