Nuclear localization of senescence marker protein-30, SMP30, in cultured mouse hepatocytes and its similarity to RNA polymerase.
Senescence marker protein-30 (SMP30), expressed mostly in the liver, protects cells against various injuries by stimulating membrane calcium-pump activity. By immunohistochemistry and western blotting, we found that SMP30 was in both the nuclei and cytoplasm of cultured mouse hepatocytes. By a homology search, we found that a domain of the SMP30 sequence 51 amino acid residues long was 60-66% similar to bacterial and yeast RNA polymerases.
Ishigami
Akihito
A
Institute of Life Sciences, Nalco Square, Chandrasekharpur, Bhubaneswar 751 023, India.
Handa
Setsuko
S
Maruyama
Naoki
N
Supakar
Prakash C
PC
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002135:Calcium-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D002848:Chromatography, DEAE-Cellulose; D003593:Cytoplasm / Q000737:chemistry; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism* / Q000648:ultrastructure*; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D015238:Sulfotransferases
D002135:Calcium-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; C513324:Rgn protein, mouse; D012321:DNA-Directed RNA Polymerases; D015238:Sulfotransferases; C028152:alcohol sulfotransferase