Binding of nitrobenzene to hepatic DNA and hemoglobin at low doses in mice.
Nitrobenzene (NB) is a widely used industrial chemical, and is considered a hazardous air pollutant. Evidence has recently showed that nitrobenzene is an animal carcinogen. We investigated the binding of 14C-NB to hepatic DNA and Hb in mice at low doses using an ultrasensitive method of accelerator mass spectrometry (AMS). In a dose-response profile, NB-DNA and NB-Hb adduct levels increased with increasing administered doses from 0.1 microg/kg b.w. to 10 mg/kg b.w. with a good linearity in a log/log presentation. At 2 h after NB administration, NB-DNA adduct levels were about twofold greater than that of NB-Hb at all doses. In the time course study NB-DNA adduct levels reduced rapidly through an exponential decay profile, whereas NB-Hb adducts showed a different decay mode, declining rather slowly to low levels. Our findings on the genotoxicity of NB do furnish a significant evidence in support of the probable carcinogenic property of NB previously reported.
Li
Hongli
H
Department of Applied Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, People's Republic of China.
Wang
Haifang
H
Sun
Hongfang
H
Liu
Yuanfang
Y
Liu
Kexin
K
Peng
Shixiang
S
D000818:Animals; D004247:DNA / Q000378:metabolism*; D018736:DNA Adducts / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006454:Hemoglobins / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D009578:Nitrobenzenes / Q000008:administration & dosage* / Q000378:metabolism*; D011485:Protein Binding; D013997:Time Factors
D018736:DNA Adducts; D006454:Hemoglobins; D009578:Nitrobenzenes; D004247:DNA; C036077:nitrobenzene