Solid state cultivation of Curvularia lunata for transformation of rifamycin B to S.
Biotransformation of rifamycin B to rifamycin S using two strains of C. lunata namely NCIM 716 and NMU grown on various solid substrates viz., grass, paper, jowar/wheat straw, bran and bagasse was studied. Almost complete biotransformation efficiency of rifamycin B at 0. 06 mM concentration was observed within 24 hr. Among these two strains, C. lunata NMU showed 90% of biotransformation and higher rate of cellulose utilization on solid substrates vis-à-vis reference strain. Cellulase activity of both strains was also studied for exoglucanase, endoglucanase and beta-glucosidase. Column bioreactor studies with bagasse revealed further improvement in biotransformation efficiency of C. lunata NMU.
Rasalkar
A A
AA
Department of Microbiology, School of Life Sciences, North Maharashtra University, PO Box 80, Jalgaon 425 001, India.
Salunke
B K
BK
Sayyed
R Z
RZ
Chaudhari
A B
AB
Chincholkar
S B
SB
D001711:Biotransformation; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D004043:Dietary Fiber / Q000378:metabolism; D043326:Glucan 1,3-beta-Glucosidase; D003904:Mitosporic Fungi / Q000254:growth & development* / Q000378:metabolism*; D006109:Poaceae / Q000737:chemistry / Q000378:metabolism; D012294:Rifamycins / Q000378:metabolism*; D014908:Triticum / Q000737:chemistry / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
D004043:Dietary Fiber; D012294:Rifamycins; C033974:rifamycin B; D002482:Cellulose; C027433:bagasse; D001617:beta-Glucosidase; D002480:Cellulase; D043326:Glucan 1,3-beta-Glucosidase; C032779:rifamycin S