Experimental conversion of virulent RH Toxoplasma gondii tachyzoites in vitro.
We aimed to induce conversion of RH-stain tachyzoites to bradyzoites by changing the pH of the culture medium. Alkalization of the medium to pH 8 induced morphological changes in the cultured tachyzoites. The majority of the organism increased in size and changed from a regular crescent shape to a rounded or ovoid shape. Cyst-like structures were formed. Using a computerized image analyser, significant differences in the size of the whole organisms and in their nuclei were observed compared to the control group. The converted organisms also showed significant differences from the control group by quantitative DNA analysis, and did not infect mice.
Hammouda
N A
NA
Department of Parasitology, Faculty of Medicine, University of Alexandria, Alexandria, Egypt.
Ibrahim
I R
IR
Elkerdany
E D
ED
Negm
A Y
AY
Allam
S R
SR
D000818:Animals; D002453:Cell Cycle; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry*; D016054:DNA, Protozoan / Q000032:analysis; D004195:Disease Models, Animal; D006863:Hydrogen-Ion Concentration; D007091:Image Processing, Computer-Assisted; D008018:Life Cycle Stages / Q000502:physiology*; D051379:Mice; D010537:Peritoneum / Q000469:parasitology; D014122:Toxoplasma / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity / Q000648:ultrastructure*; D014123:Toxoplasmosis / Q000469:parasitology*
D003470:Culture Media; D016054:DNA, Protozoan