title: Multimethod characterization of the interaction of aluminum ion with alpha-ketoglutaric acid in acidic aqueous solutions.
abstract: It has recently been reported that aluminum plays a very important role in reducing the activity of Krebs-cycle enzymes and glutamate dehydrogenase in rat brain homogenate. Therefore, it is necessary to identify the aluminum binding ability with the pivotal substrate alpha-ketoglutarate in biological systems. The interactions of aluminum with alpha-ketoglutarate were studied with pH-potentiometry, cyclic voltammetry, UV-vis, 1H, 27Al-NMR and Raman spectra multi-analytical techniques in acidic aqueous solution to measure the stoichiometries and stability constants of the complexes and its keto-enol tautomerism. The alpha-ketoglutarate was found to bind Al in a bidentate manner at the carboxylate and carbonyl moieties. The mononuclear 1:1 (AlLH(-1), AIL+, AlHL2+) and 2:1 (AlL2-, AlL2H(-2)3-) species, and dinuclear 2:1 (Al2L4+) species were found in acidic aqueous solution. Meanwhile, Al can promote alpha-KG tautomerize to its enolic-structure compounds in solutions. These findings may help to further understand the influence of Al on GDH enzyme reactions in biological systems.
authors.affiliation: State Key Laboratory of Coordination Chemistry, Department of Chemistry, Nanjing University, Nanjing 210093, P. R. China.
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Yang
authors.forename: Shuping
authors.initials: S
authors.lastname: Bi
authors.forename: Xianlong
authors.initials: X
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Bai
mesh_terms: D000535:Aluminum / Q000493:pharmacokinetics* / Q000633:toxicity; D001665:Binding Sites; D004563:Electrochemistry / Q000379:methods; D015386:Hazardous Substances / Q000493:pharmacokinetics; D006863:Hydrogen-Ion Concentration; D007656:Ketoglutaric Acids / Q000378:metabolism*; D015394:Molecular Structure; D012996:Solutions; D013057:Spectrum Analysis
chemical_list: D015386:Hazardous Substances; D007656:Ketoglutaric Acids; D012996:Solutions; D000535:Aluminum