The power and prospects of fluorescence microscopies and spectroscopies.
Recent years have witnessed a renaissance of fluorescence microscopy techniques and applications, from live-animal multiphoton confocal microscopy to single-molecule fluorescence spectroscopy and imaging in living cells. These achievements have been made possible not so much because of improvements in microscope design, but rather because of development of new detectors, accessible continuous wave and pulsed laser sources, sophisticated multiparameter analysis on one hand, and the development of new probes and labeling chemistries on the other. This review tracks the lineage of ideas and the evolution of thinking that have led to the actual developments, and presents a comprehensive overview of the field, with emphasis put on our laboratory's interest in single-molecule microscopy and spectroscopy.
Michalet
Xavier
X
Department of Chemistry and Biochemistry, UCLA, Young Hall, 607 Charles E. Young Drive East, Los Angeles, California 90095, USA. michalet@chem.ucla.edu
Kapanidis
Achillefs N
AN
Laurence
Ted
T
Pinaud
Fabien
F
Doose
Soeren
S
Pflughoefft
Malte
M
Weiss
Shimon
S
D004867:Equipment Design; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D013194:Staining and Labeling / Q000295:instrumentation / Q000379:methods
D005456:Fluorescent Dyes