Hypoglycemic effect of methanol extract of Phyllanthus amarus Schum & Thonn on alloxan induced diabetes mellitus in rats and its relation with antioxidant potential.
Methanolic extract of P. amarus was found to have potential anti-oxidant activity as it could inhibit lipid peroxidation, and scavenge hydroxyl and superoxide radicals in vitro. The amount required for 50% inhibition of lipid peroxide formation was 104 microg/ml and the concentrations needed to scavenge hydroxyl and superoxide radicals were 117 and 19 microg/ml respectively. The extract was found to reduce the blood sugar in alloxan diabetic rats at 4th hr by 6% at a dose level of 200 mg/kg body wt and 18.7% at a concentration of 1000 mg/kg body wt. Continued administration of the extract for 15 days produced significant (P < 0.001) reduction in blood sugar. On 18th day after alloxan administration values were almost similar to normal in the group taking 1000 mg/kg body wt.
Raphael
K Regi
KR
Amala Cancer Research Center, Amalanagar, Thrissur 680 553, India.
Sabu
M C
MC
Kuttan
Ramadasan
R
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000627:therapeutic use; D017665:Hydroxyl Radical / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D000432:Methanol / Q000737:chemistry; D029803:Phyllanthus / Q000737:chemistry*; D010936:Plant Extracts / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism
D000975:Antioxidants; D001786:Blood Glucose; D016166:Free Radical Scavengers; D007004:Hypoglycemic Agents; D008054:Lipid Peroxides; D010936:Plant Extracts; D013481:Superoxides; D017665:Hydroxyl Radical; D000432:Methanol