title: Comparative effects of basophil-directed growth factors.
abstract: IL-3, IL-5, and GM-CSF exert various overlapping functions in basophils. We investigated the receptor expression profiles and concentration-dependent effects of IL-3, IL-5, and GM-CSF on several basophil functions in comparison with their effects on eosinophils. The order of the receptor expression levels was IL-3Ralpha>IL-5Ralpha>GM-CSFRalpha in basophils and IL-5Ralpha>or=GM-CSFRalpha>IL-3Ralpha in eosinophils. Compared with eosinophils, basophils expressed a much higher level of IL-3Ralpha and similar levels of IL-5Ralpha and GM-CSFRalpha. The order of potency was IL-3>IL-5=GM-CSF for degranulation, survival, and CD11b expression in basophils, and IL-5=GM-CSF>or=IL-3 for survival and CD11b expression in eosinophils. However, IL-3 induced CD69 expression preferentially in basophils. Our results indicate that IL-3 is the most potent activator of human basophils, and that the rank order of potency of hemopoietic growth factors virtually corresponded to their receptor expression levels in both cell types.
authors.affiliation: Department of Allergy and Rheumatology, University of Tokyo, Graduate School of Medicine, Japan.
authors.forename: Chitose
authors.initials: C
authors.lastname: Yoshimura-Uchiyama
authors.forename: Masao
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nagase
authors.forename: Takao
authors.initials: T
authors.lastname: Fujisawa
authors.forename: Chisei
authors.initials: C
authors.lastname: Ra
authors.forename: Kouji
authors.initials: K
authors.lastname: Matsushima
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Iwata
authors.forename: Takashi
authors.initials: T
authors.lastname: Igarashi
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Koichi
authors.initials: K
authors.lastname: Hirai
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis; D001491:Basophils / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D039481:CD11b Antigen / Q000096:biosynthesis; D015550:Cell Degranulation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004804:Eosinophils / Q000187:drug effects / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D016023:Integrins / Q000096:biosynthesis; D007377:Interleukin-3 / Q000494:pharmacology*; D053650:Interleukin-3 Receptor alpha Subunit; D015848:Interleukin-5 / Q000494:pharmacology*; D037181:Lectins, C-Type; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D016185:Receptors, Interleukin-3 / Q000096:biosynthesis; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D039481:CD11b Antigen; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D016023:Integrins; D007377:Interleukin-3; D053650:Interleukin-3 Receptor alpha Subunit; D015848:Interleukin-5; D037181:Lectins, C-Type; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D016185:Receptors, Interleukin-3; D000099192:CD69 Antigens; C115022:IL3RA protein, human