[Vascular endothelial growth factor gene expression in nasopharyngeal carcinoma cell line induced by hypoxia in vitro].
OBJECTIVE
Hypoxia is a common phenomenon in the tumor microenvironment of most solid tumors including human nasopharyngeal neoplasms. Hypoxia can induces vascular endothelial growth factor (VEGF) gene expression in some tumor cells like glioma cell line C6. The present investigations were conducted to study the effect of hypoxia on the VEGF gene expression in cultured nasopharyngeal carcinoma (NPC) cell line CNE-2Z in vitro and to discuss its impact on tumor metastasis.


METHODS
Hypoxic culture model in vitro was established to test the VEGF gene expression in CNE-2Z cell line. After cultured in the hypoxia circumstance for 24 hours, VEGF expression were tested in mRNA level and protein level by RT-PCR and Western-blot analysis, respectively.


RESULTS
VEGF mRNA isoforms VEGF189, VEGF165, VEGF145,and VEGF121 were identified by RT-PCR in cultured CNE-2Z cell line. All of the four VEGF mRNA isoforms expression were increased after treated under hypoxia for 24 hours. The mRNA level of four VEGF isoforms were 2.67+/-0.30, 2.05+/-0.03, 2.73+/-0.15, and 1.65+/-0.01 folds as those in normoxic cells,respectively. Western blot analysis showed that VEGF protein expression in CNE-2Z cells was 2.20+/-0.07 folds as that in normoxic cells.


CONCLUSIONS
VEGF gene expresses in CNE-2Z cell line and can be induced by hypoxia in vitro, this mechanism may be involve in metastasis process of NPC by inproved neoplasm angiogenesis.
Yang
Hai-Feng
HF
Department of Pathology, Guangdong Medical College, Zhanjiang, Guangdong, 524023, PR China.
Tang
Wei-Ping
WP
D015687:Cell Hypoxia; D016228:Endothelial Growth Factors / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D008222:Lymphokines / Q000096:biosynthesis* / Q000235:genetics; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D009362:Neoplasm Metastasis; D010100:Oxygen / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D010100:Oxygen