title: Enzyme immunoassay detection of platelet-derived microparticles and RANTES in acute coronary syndrome.
abstract: Platelet-derived microparticles (PDMPs) are produced by platelet activation or physical stimulation under various conditions. To evaluate changes in platelet and chemokine function in patients undergoing percutaneous transluminal coronary angioplasty (PTCA), we measured and compared levels of PDMPs and a C-C chemokine, regulated on activation normally T-cell express and secreted (RANTES), by ELISA. Levels of PDMP and RANTES in patients with acute coronary syndrome were significantly higher than those in the control groups (PDMP: 20.1 +/- 2.9 vs 80.4 +/- 7.3 U/ml, p < 0.001; RANTES: 18.6 +/- 3.7 vs 52.1 +/- 4.6 ng/ml, p < 0.01), but did not differ between the control groups and patients with stable angina. PDMP levels were higher in patients with acute myocardial infarction (AMI) than in patients with unstable angina (PDMP: 115.0 +/- 7.1 vs 63.9 +/- 6.2 U/ml, p < 0.001). There was no difference in the RANTES levels, however, between patients with AMI and patients with unstable angina. PDMP and RANTES levels were significantly decreased after PTCA (PDMP, p < 0.001; RANTES, p < 0.05), but without differences between the two groups. In addition, the level of PDMP was significantly correlated with that of RANTES or soluble CD40 ligand. These findings suggest that PTCA may prevent the development of AMI-associated complications in which activated platelets and RANTES play roles. Our ELISA method appears to be sufficient for monitoring PDMP and RANTES levels after PTCA in patients with acute coronary syndrome.
authors.affiliation: The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan. shosaku-n@mbp.shere.ne.jp
authors.forename: Shosaku
authors.initials: S
authors.lastname: Nomura
authors.forename: Shigeru
authors.initials: S
authors.lastname: Uehata
authors.forename: Sonoko
authors.initials: S
authors.lastname: Saito
authors.forename: Kazuoki
authors.initials: K
authors.lastname: Osumi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ozeki
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kimura
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D000787:Angina Pectoris / Q000097:blood; D000789:Angina, Unstable / Q000097:blood; D015906:Angioplasty, Balloon, Coronary; D001792:Blood Platelets / Q000648:ultrastructure*; D023201:CD40 Ligand / Q000097:blood; D016022:Case-Control Studies; D018946:Chemokine CCL5 / Q000097:blood*; D003327:Coronary Disease / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000628:therapy; D019007:P-Selectin / Q000097:blood; D010316:Particle Size; D015539:Platelet Activation; D010974:Platelet Aggregation; D013577:Syndrome
chemical_list: D018946:Chemokine CCL5; D019007:P-Selectin; D023201:CD40 Ligand