title: Interisland evolution of Trimeresurus flavoviridis venom phospholipase A(2) isozymes.
abstract: Trimeresurus flavoviridis snakes inhabit the southwestern islands of Japan. A phospholipase A(2) (PLA(2)), named PL-Y, was isolated from Okinawa T. flavoviridis venom and its amino acid sequence was determined from both protein and cDNA. PL-Y was unable to induce edema. In contrast, PLA-B, a PLA(2) from Tokunoshima T. flavoviridis venom, which is different at only three positions from PL-Y, is known to induce edema. A new PLA(2), named PLA-B', which is similar to PLA-B, was cloned from Amami-Oshima T. flavoviridis venom gland. Three T. flavoviridis venom basic [Asp(49)]PLA(2) isozymes, PL-Y (Okinawa), PLA-B (Tokunoshima), and PLA-B' (Amami-Oshima), are identical in the N-terminal half but have one to four amino acid substitutions in the beta1-sheet and its vicinity. Such interisland sequence diversities among them are due to isolation in the different environments over 1 to 2 million years and appear to have been brought about by natural selection for point mutation in their genes. Otherwise, a major PLA(2), named PLA2, ubiquitously exists in the venoms of T. flavoviridis snakes from the three islands with one to three synonymous substitutions in their cDNAs. It is assumed that the PLA2 gene is a prototype among T. flavoviridis venom PLA(2) isozyme genes and has hardly undergone nonsynonymous mutation as a principal toxic component. Phylogenetic analysis based on the amino acid sequences revealed that T. flavoviridis PLA(2) isozymes are clearly separated into three groups, PLA2 type, basic [Asp(49)]PLA(2) type, and [Lys(49)]PLA(2) type. Basic [Asp(49)]PLA(2)-type isozymes may manifest their own particular toxic functions different from those of the isozymes of the PLA2 type and [Lys(49)]PLA(2) type.
authors.affiliation: Department of Applied Life Science, Faculty of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan. chijiwa@life.sojo-u.ac.jp
authors.forename: Takahito
authors.initials: T
authors.lastname: Chijiwa
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Ogawa
authors.forename: Masanobu
authors.initials: M
authors.lastname: Deshimaru
authors.forename: Ikuo
authors.initials: I
authors.lastname: Nobuhisa
authors.forename: Kinichi
authors.initials: K
authors.lastname: Nakashima
authors.forename: Naoko
authors.initials: N
authors.lastname: Oda-Ueda
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Fukumaki
authors.forename: Shosaku
authors.initials: S
authors.lastname: Hattori
authors.forename: Motonori
authors.initials: M
authors.lastname: Ohno
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003435:Crotalid Venoms / Q000201:enzymology / Q000235:genetics*; D019143:Evolution, Molecular; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000235:genetics*; D054467:Phospholipases A2; D010802:Phylogeny; D017841:Trimeresurus / Q000235:genetics*
chemical_list: D003435:Crotalid Venoms; D007527:Isoenzymes; C029748:Trimeresurus venoms; D010741:Phospholipases A; D054467:Phospholipases A2