Polymer functionalized submicrometric emulsions as potential synthetic DNA vectors.
Triglyceride-based emulsions were first prepared by a solvent displacement procedure which was modified to achieve their functionalization by surface deposition of various amphiphilic comb-like copolymers. These emulsions have been characterized as regards to hydrodynamic particle size and surface charges using dynamic light scattering and electrophoretic mobility measurements. The adsorption isotherms of a polydT15 oligonucleotide and a model plasmid showed that the process was dependent on the nature of the interfaces, the affinity for the nucleic acid increasing with more cationic charges, together with improved accessibility. The binding process was found to proceed according to two regimes: one at low nucleic acid coverage, independent of the initial plasmid concentration, and the second one at high coverage, which was nucleic-acid-concentration dependent. This behavior was considered to occur because of the development of repulsive interactions upon increasing the amount of immobilized nucleic acid. The complexation of plasmid complexed at the interface was finally investigated using the ethidium bromide displacement technique. The level of compaction of plasmid complexed onto the functionalized emulsions was lower than that obtained with the parent free polymer.
Trimaille
Thomas
T
UMR 2142, CNRS-bioMérieux, ENS-Lyon, 46 allée d'Italie, 69364 Lyon Cedex 07, France.
Chaix
Carole
C
Pichot
Christian
C
Delair
Thierry
T
D000327:Adsorption; D004247:DNA / Q000737:chemistry* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004655:Emulsions; D004996:Ethidium / Q000494:pharmacology; D005822:Genetic Vectors; D007364:Intercalating Agents / Q000494:pharmacology; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D011485:Protein Binding
D004655:Emulsions; D007364:Intercalating Agents; D009841:Oligonucleotides; D011108:Polymers; D004247:DNA; D004996:Ethidium