[The expressions of telomerase activity and cyclinD1 protein in laryngeal squamous cell carcinoma].
OBJECTIVE
In order to investigate the telomerase activity and its relationship with the overexpression of cyclin D1 protein in laryngeal squamous cell carcinomas (LSCC).


METHODS
TRAP(Telomeric repeat amplification protocol) assay and immunohistochemical method were used respectively to detect the telomerase activity and the overexpression of cyclin D1 protein in 38 LSCC and correspondent laryngeal mucosa.


RESULTS
The expressions of telomerase and cyclin D1 protein were 82%(31/38), 50%(19/38) respectively in LSCC, both of them were not associated with the age of patients, location of tumors, T stage and pathological grade(P > 0.05), but there was a significant difference among clnic stages (P < 0.05) in telomerase activity, no significant difference could be found among clinic stages in expression of cyclin D1. 18 out of 31 cases were expressed in both of them simultaneously there was significant association between them(P < 0.05).


CONCLUSIONS
Telomerase is widely activated in LSCC, the overexpression of cyclin D1 protein may be one of the most important mechanisms of telomerase activation in LSCC.
Yu
Zilong
Z
EENT Hospital, Shanghai Fudan University, Shanghai 200031.
Wang
Wei
W
D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D019938:Cyclin D1 / Q000096:biosynthesis*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D019098:Telomerase / Q000378:metabolism*
D019938:Cyclin D1; D019098:Telomerase