Antibodies to advanced glycation end products in children with diabetes mellitus.
The tissue accumulation of advanced glycation end products (AGE) alters the structure and function of long-lived proteins. A number of studies have shown that tissue accumulation of AGE correlates with the severity of diabetic complications. Proteins containing AGE are highly immunogenic and anti-AGE antibodies were found in sera of diabetic rats and human. Considering the potential use of anti-AGE antibodies as a marker of AGE deposition during diabetes, we have investigated, by competitive ELISA, the presence of anti-AGE antibodies in sera of 58 children with Type 1 diabetes mellitus. The patients were studied for the period of 5 years. Positive for anti-AGE antibodies were 19 children with diabetes. Fourteen of them showed initial data for vascular complications. Anti-AGE antibodies were related to age (r = .25, P = .024), duration of diabetes (r = .41, P = .0001), HbA1c (r = .27, P = .016), microalbuminuria (r = .41, P = .0001), retinopathy (r = .35, P = .001), triglycerides (r = .27, P = .016), and total cholesterol (r = .19, P = .05). In conclusion, our study showed that the investigation of the levels and dynamics of anti-AGE antibodies might give the possibility for early diagnosis and prognosis of the severity of diabetic late complications.
Nicoloff
G
G
Department of Biology and Pathological Physiology, University School of Medicine, 1, St. Kliment Ohridski Street, 5800 Pleven, Bulgaria. nicoloff_bg@yahoo.com
Baydanoff
S
S
Petrova
Ch
Ch
Christova
P
P
D000293:Adolescent; D000704:Analysis of Variance; D001323:Autoantibodies / Q000097:blood*; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D005260:Female; D017127:Glycation End Products, Advanced / Q000097:blood* / Q000276:immunology*; D006801:Humans; D008297:Male
D001323:Autoantibodies; D017127:Glycation End Products, Advanced