title: Laser-guided direct writing: a novel method to deposit biomolecules for biosensors arrays.
abstract: In this paper, we present a potential biomolecular patterning method, laser-guided direct writing guidance (LGDW), which may be utilized to deposit organic and bioactive particles for biosensor arrays. The instrumentation and operation of the LGDW system is introduced and the system settings used to achieve deposition are reported. The biomolecule, avidin, was deposited onto a substrate using LGDW to evaluate the possible damage from the laser on the biomolecules. The functionality of avidin after laser-based guidance was examined by exposing the deposited avidin molecules to its ligand, biotin. The results show some avidin retained its affinity to biotin after LGDW demonstrating little damage to the biomolecules.
authors.affiliation: Department of Biological Engineering, University of Missouri-Columbia, 250 Ag. Bldg., Columbia, MO 65211, USA.
authors.forename: Juntao
authors.initials: J
authors.lastname: Xu
authors.forename: Sheila A
authors.initials: SA
authors.lastname: Grant
authors.forename: Robert L
authors.initials: RL
authors.lastname: Pastel
mesh_terms: D000818:Animals; D001360:Avidin / Q000737:chemistry; D001671:Biochemistry / Q000295:instrumentation / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D002417:Cattle; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007834:Lasers; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D055095:Optics and Photonics / Q000295:instrumentation*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013314:Stress, Mechanical
chemical_list: D001360:Avidin; D012710:Serum Albumin, Bovine; D001710:Biotin