Development of an in vitro method for the evaluation of candidate repellents against Leptotrombidium (Acari: Trombiculidae) chiggers.
We developed a rapid and economical in vitro procedure with which to evaluate the efficacy of candidate repellents against chiggers. The procedure requires only 5 min and a small number of chiggers to obtain a valid estimate of the median effective dose. We used this procedure to evaluate the repellent activity of 11 compounds against the chigger, Leptotrombidium imphalum Vercammen-Grandjean and Langston. Median effective doses were determined for 10 of the 11 compounds. DM-165-2 (N,N-diethyl-3-flurobenzamide) was the only compound that was significantly more effective than deet.
Lerdthusnee
Kriangkrai
K
Department of Entomology, US Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
Khlaimanee
Nittaya
N
Monkanna
Taweesak
T
Mungviriya
Siriporn
S
Leepitakrat
Warisa
W
Debboun
Mustapha
M
Coleman
Russell E
RE
D000818:Animals; D001179:Arthropod Vectors; D003671:DEET / Q000633:toxicity; D007302:Insect Repellents / Q000633:toxicity*; D013329:Structure-Activity Relationship; D014324:Trombiculidae / Q000187:drug effects*
D007302:Insect Repellents; D003671:DEET