title: [Effect of P fimbriae on pyuria and bacterial colonization of the human urinary tract].
abstract: This study investigated the role of P fimbriae in colonization of Escherichia coli, host response, and bacterial persistence in humans. Human volunteers were inoculated intravesically with the nonadherent ABU isolate E. coli 83972 and with P fimbriated transformants of the same strain. During the following 24 h all urine samples, and thereafter daily samples, were collected for urine culture, analysis of neutrophil numbers, and cytokine concentrations (IL-6 and IL-8). The P fimbriated transformants showed enhanced bacterial colonization in comparison to E. coli 83972 and lowered the bacterial numbers needed for persistent bacteriuria. The P fimbriated transformants also lowered the bacterial numbers needed for a significant neutrophil and cytokine host response. We conclude that P fimbriae enhance bacterial colonization and trigger the host response in the human urinary tract.
authors.affiliation: Department of Urology, Lund University Hospital, Lund, Sweden. bjorn.wullt@urokir.lu.se
authors.forename: B
authors.initials: B
authors.lastname: Wullt
authors.forename: G
authors.initials: G
authors.lastname: Bergsten
authors.forename: M
authors.initials: M
authors.lastname: Samuelsson
authors.forename: C
authors.initials: C
authors.lastname: Svanborg
mesh_terms: D000328:Adult; D000668:Ampicillin Resistance / Q000235:genetics; D001422:Bacterial Adhesion / Q000502:physiology*; D002675:Child, Preschool; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology*; D005260:Female; D010861:Fimbriae, Bacterial / Q000235:genetics* / Q000276:immunology; D005838:Genotype; D006801:Humans; D015850:Interleukin-6 / Q000652:urine; D016209:Interleukin-8 / Q000652:urine; D007613:Kanamycin Resistance / Q000235:genetics; D007958:Leukocyte Count; D008297:Male; D009504:Neutrophils / Q000276:immunology; D011776:Pyuria / Q000382:microbiology*; D011971:Receptors, Immunologic / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics; D014552:Urinary Tract Infections / Q000276:immunology / Q000382:microbiology*; D014774:Virulence / Q000235:genetics
chemical_list: D015850:Interleukin-6; D016209:Interleukin-8; D011971:Receptors, Immunologic; C037872:pili, bacterial receptor