title: Tumor initiating activity of Helicobacter pylori water extract on mouse skin carcinogenesis.
abstract: Helicobacter pylori (H. pylori) infection has been associated with gastric carcinogenesis, but responsible and detail mechanisms are insufficient by the absence of adequate data. To obtain direct evidence regarding the carcinogenicity of H. pylori, we investigated the initiating and promoting activity of H. pylori water extract (HPE) in two-stage mouse skin carcinogenesis model. HPE treatment, as an initiation, significantly enhanced tumor formation compared with control group. Moreover, HPE treatment increased production of 8-hydroxydeoxyguanosine in epidermal cells and HPE-initiated/TPA-promoted papillomas demonstrated a point mutation of the Ha-ras gene. These results suggest an initiating activity of HPE on two-stage mouse skin carcinogenesis.
authors.affiliation: First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Norimasa
authors.initials: N
authors.lastname: Yoshida
authors.forename: Harukuni
authors.initials: H
authors.lastname: Tokuda
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Ichiishi
authors.forename: Masashi
authors.initials: M
authors.lastname: Kuchide
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kokura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Naito
authors.forename: Shinya
authors.initials: S
authors.lastname: Toyokuni
authors.forename: Hoyoku
authors.initials: H
authors.lastname: Nishino
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Yoshikawa
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000483:Alleles; D000818:Animals; D002273:Carcinogens / Q000302:isolation & purification / Q000633:toxicity*; D002458:Cell Fractionation; D003043:Cocarcinogenesis; D003062:Codon / Q000187:drug effects; D004247:DNA / Q000187:drug effects / Q000235:genetics; D018736:DNA Adducts / Q000032:analysis; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D018450:Disease Progression; D005260:Female; D011905:Genes, ras / Q000187:drug effects; D016480:Helicobacter pylori / Q000737:chemistry*; D008163:Luminescent Measurements; D051379:Mice; D018632:Mice, Inbred SENCAR; D018384:Oxidative Stress; D010212:Papilloma / Q000139:chemically induced* / Q000235:genetics; D009195:Peroxidase / Q000032:analysis; D017354:Point Mutation; D012867:Skin / Q000187:drug effects / Q000201:enzymology; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000235:genetics; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity; D014867:Water
chemical_list: D002273:Carcinogens; D003062:Codon; D018736:DNA Adducts; D014867:Water; D013481:Superoxides; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D009195:Peroxidase; D003849:Deoxyguanosine; D013755:Tetradecanoylphorbol Acetate