title: Entirely automated quantification of human immunodeficiency virus type 1 (HIV-1) RNA in plasma by using the ultrasensitive COBAS AMPLICOR HIV-1 monitor test and RNA purification on the MagNA pure LC instrument.
abstract: The ultrasensitive COBAS AMPLICOR HIV-1 Monitor test was complemented with automated RNA purification on the MagNA Pure LC instrument. This enabled entirely automated ultrasensitive assessment of viral loads in human immunodeficiency virus type 1 (HIV-1)-infected individuals. The detection limit of the fully automated assay and the viral load measurements in 80 clinical samples were found to be in good agreement with those of the conventional ultrasensitive COBAS AMPLICOR HIV-1 Monitor test. The fully automated assay showed markedly reduced hands-on time and was found to be suitable for the routine assessment of HIV-1 viral loads in a clinical diagnostic laboratory.
authors.affiliation: Institute of Laboratory Medicine, General Hospital Linz, A-4020 Linz, Austria.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Hölzl
authors.forename: Markus
authors.initials: M
authors.lastname: Stöcher
authors.forename: Victoria
authors.initials: V
authors.lastname: Leb
authors.forename: Herbert
authors.initials: H
authors.lastname: Stekel
authors.forename: Jörg
authors.initials: J
authors.lastname: Berg
mesh_terms: D001331:Automation; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D012367:RNA, Viral / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D019562:Viral Load
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic