[The primary culture methods of colorectal adenoma cells].
OBJECTIVE
To improve the primary cell culture methods of colorectal adenomas.


METHODS
The methods of the in vitro primary cell culture of colorectal adenomas, mainly the method for taking and preparing specimen, the methods of culture conditions and cells purification, were considered again and improved in this study.


RESULTS
Of the 32 cases, of polyp, 26 were successfully cultured and 6 failed. The culture lasted 5-23 days with 50%-95% viscosity of wells. By means of immuno-histochemical staining and electromicroscopy, the cultured cells were proved to be of epithelial origin.


CONCLUSIONS
The primary culture methods of adenoma cells have been improved, but further study on the serial transfer culture cell lines is most needed.
Zhang
Zhihong
Z
Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, China.
Ouyang
Qin
Q
Qiu
Chunhua
C
Li
Shengfu
S
D000236:Adenoma / Q000473:pathology*; D003110:Colonic Neoplasms / Q000473:pathology*; D003111:Colonic Polyps / Q000473:pathology*; D003470:Culture Media; D005260:Female; D006801:Humans; D008297:Male; D013048:Specimen Handling; D014407:Tumor Cells, Cultured
D003470:Culture Media