Gene expression profile of mouse bone marrow stromal cells determined by cDNA microarray analysis.
Bone marrow stromal cells (BMSC) have gained increased attention because of their multipotency and adult stem cell character. They have been shown to differentiate into other cell types of the mesenchymal lineage and also into non-mesenchymal cells. The exact identity of the original cells, which are isolated from bone marrow by their selective adherence to plastic, remains unknown to date. We have established and characterized mouse BMSC cultures and analyzed three independent samples by cDNA microarrays. The expression profile was compared with two previous expression studies of human BMSC and revealed a high degree of concordance between different techniques and species. To gain clues about the positional context and biology of the isolated cells within the bone marrow stroma, we searched our data for genes that encode proteins of the extracellular matrix, cell adhesion proteins, cytoskeletal proteins and cytokines/cytokine receptors. This analysis revealed a close association of BMSC with vascular cells and indicated that BMSC resemble pericytes.
Wieczorek
Georg
G
Max-Planck-Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany.
Steinhoff
Christine
C
Schulz
Ralph
R
Scheller
Marina
M
Vingron
Martin
M
Ropers
H-Hilger
HH
Nuber
Ulrike A
UA
D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000235:genetics; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D003001:Cloning, Molecular; D016207:Cytokines / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000235:genetics; D017931:DNA Primers; D016326:Extracellular Matrix Proteins / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D018121:Receptors, Cytokine / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology*
D015815:Cell Adhesion Molecules; D016207:Cytokines; D003598:Cytoskeletal Proteins; D017931:DNA Primers; D016326:Extracellular Matrix Proteins; D018121:Receptors, Cytokine; D012313:RNA