title: Genome-wide screening for complete genetic loss in prostate cancer by comparative hybridization onto cDNA microarrays.
abstract: We demonstrate that comparative genomic hybridization (CGH) onto cDNA microarrays may be used to carry out genome-wide screens for regions of genetic loss, including homozygous (complete) deletions that may represent the possible location of tumour suppressor genes in human cancer. Screening of the prostate cancer cell lines LNCaP, PC3 and DU145 allowed the mapping of specific regions where genome copy number appeared altered and led to the identification of two novel regions of complete loss at 17q21.31 (500 kb spanning STAT3) and at 10q23.1 (50-350 kb spanning SFTPA2) in the PC3 cell line.
authors.affiliation: Molecular Carcinogenesis Section, Male Urological Cancer Research Center, Institute of Cancer Research, Sutton, Surrey, UK. csc@icr.ac.uk
authors.forename: Jeremy
authors.initials: J
authors.lastname: Clark
authors.forename: Sandra
authors.initials: S
authors.lastname: Edwards
authors.forename: Andrew
authors.initials: A
authors.lastname: Feber
authors.forename: Penny
authors.initials: P
authors.lastname: Flohr
authors.forename: Megan
authors.initials: M
authors.lastname: John
authors.forename: Ian
authors.initials: I
authors.lastname: Giddings
authors.forename: Sue
authors.initials: S
authors.lastname: Crossland
authors.forename: Michael R
authors.initials: MR
authors.lastname: Stratton
authors.forename: Richard
authors.initials: R
authors.lastname: Wooster
authors.forename: Colin
authors.initials: C
authors.lastname: Campbell
authors.forename: Colin S
authors.initials: CS
authors.lastname: Cooper
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D017353:Gene Deletion; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000737:chemistry
chemical_list: D018076:DNA, Complementary; D004273:DNA, Neoplasm