A sensitive bioassay for chicken interleukin-18 based on the inducible release of preformed interferon-gamma.
Conventional tests for measuring the biological activity of chicken interleukin (IL)-18 require primary chicken spleen cells. We now describe a sensitive bioassay that is based on interleukin-18-induced release of interferon (IFN)-gamma by a permanent chicken cell line. In B19-2D8 cells, cytoplasmically stored interferon-gamma is quickly secreted in response to interleukin-18 exposure.
Puehler
Florian
F
Department of Virology, University of Freiburg, Hermann-Herder-Str. 11, 79104, Freiburg, Germany.
Göbel
Thomas
T
Breyer
Ulrike
U
Ohnemus
Annette
A
Staeheli
Peter
P
Kaspers
Bernd
B
D000818:Animals; D001681:Biological Assay; D002461:Cell Line, Transformed; D002645:Chickens / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D007371:Interferon-gamma / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D020382:Interleukin-18 / Q000494:pharmacology*; D007700:Kinetics; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic
D020382:Interleukin-18; D007371:Interferon-gamma