title: Distribution of new human beta-defensin genes clustered on chromosome 20 in functionally different segments of epididymis.
abstract: Human beta-defensins are a family of cationic peptides that share a pattern of six conserved cysteine residues. We describe the cloning and characterization of the cDNAs of five novel beta-defensin genes (DEFB25-DEFB29) clustered on chromosome 20p13, which were identified using a bioinformatics approach. Expression analysis revealed the occurrence of the transcripts in only a few organs, with the highest abundance in the male genital tract. Examination of beta-defensin expression in human epididymis by real-time quantitative RT-PCR revealed a distribution along the functionally different segments of the epididymal duct. In situ hybridization for one of the cDNAs shows mRNA restriction to the epithelial cell layer of the epididymis, known to secrete factors responsible for sperm maturation. We suggest that the novel peptides carry out physiological functions in the male genital tract that may not be directly related to bacterial growth inhibition in host defense.
authors.affiliation: IPF PharmaCeuticals GmbH, Feodor-Lynen-Str. 31, 30625 Hannover, Germany.
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Rodríguez-Jiménez
authors.forename: Alexander
authors.initials: A
authors.lastname: Krause
authors.forename: Sandra
authors.initials: S
authors.lastname: Schulz
authors.forename: Wolf Georg
authors.initials: WG
authors.lastname: Forssmann
authors.forename: José Ramón
authors.initials: JR
authors.lastname: Conejo-Garcia
authors.forename: Rainer
authors.initials: R
authors.lastname: Schreeb
authors.forename: Dirk
authors.initials: D
authors.lastname: Motzkus
mesh_terms: D000595:Amino Acid Sequence; D002890:Chromosomes, Human, Pair 20; D003001:Cloning, Molecular; D004822:Epididymis / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016415:Sequence Alignment; D023083:beta-Defensins / Q000235:genetics* / Q000378:metabolism
chemical_list: D023083:beta-Defensins