title: A sensitive bioassay for chicken interleukin-18 based on the inducible release of preformed interferon-gamma.
abstract: Conventional tests for measuring the biological activity of chicken interleukin (IL)-18 require primary chicken spleen cells. We now describe a sensitive bioassay that is based on interleukin-18-induced release of interferon (IFN)-gamma by a permanent chicken cell line. In B19-2D8 cells, cytoplasmically stored interferon-gamma is quickly secreted in response to interleukin-18 exposure.
authors.affiliation: Department of Virology, University of Freiburg, Hermann-Herder-Str. 11, 79104, Freiburg, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Puehler
authors.forename: Thomas
authors.initials: T
authors.lastname: Göbel
authors.forename: Ulrike
authors.initials: U
authors.lastname: Breyer
authors.forename: Annette
authors.initials: A
authors.lastname: Ohnemus
authors.forename: Peter
authors.initials: P
authors.lastname: Staeheli
authors.forename: Bernd
authors.initials: B
authors.lastname: Kaspers
mesh_terms: D000818:Animals; D001681:Biological Assay; D002461:Cell Line, Transformed; D002645:Chickens / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D007371:Interferon-gamma / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D020382:Interleukin-18 / Q000494:pharmacology*; D007700:Kinetics; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic
chemical_list: D020382:Interleukin-18; D007371:Interferon-gamma