title: Chlamydia antibody response in healthy volunteers immunized with nonchlamydial antigens: a randomized, double-blind, placebo-controlled study.
abstract: Serological analysis is often used for the diagnosis of chlamydial infections. However, an increase in Chlamydia antibodies has been reported in patients with parvovirus and Mycoplasma infections. Whether this antibody response is the result of dual infection or nonchlamydial antigen stimulation is unknown. In a randomized study, 48 healthy volunteers either were immunized against yellow fever, polio, diphtheria, and tetanus (the group receiving intervention with nonchlamydial antigen) or received saline injections (the placebo group). The change in antibody levels was compared between the 2 groups. The Chlamydia recombinant lipopolysaccharide enzyme-linked immunosorbent assay (Medac) showed an increase in the antibody titer in the intervention group, compared with that in the control group (for immunoglobulin M, P=.004; for immunoglobulin A, P=.038; and for immunoglobulin G, P=.056), but no differences between study groups was found when the C. pneumoniae enzyme immunoassay (EIA; ThermoLabsystems), the C. pneumoniae EIA (Medac), and the microimmunofluorescence test (MRL) were used. An increase in antibodies to Chlamydia organisms can be measured after exposure to nonchlamydial antigens, depending on the test used.
authors.affiliation: Research Unit Q, Department of Infectious Diseases, Skejby Sygehus, Aarhus University Hospital, Denmark. oes@sks.aaa.dk
authors.forename: Stine
authors.initials: S
authors.lastname: Johnsen
authors.forename: Paul L
authors.initials: PL
authors.lastname: Andersen
authors.forename: Gerold
authors.initials: G
authors.lastname: Stanek
authors.forename: Gunna
authors.initials: G
authors.lastname: Christiansen
authors.forename: Svend
authors.initials: S
authors.lastname: Birkelund
authors.forename: Lene M
authors.initials: LM
authors.lastname: Berthelsen
authors.forename: Lars
authors.initials: L
authors.lastname: Østergaard
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis*; D000941:Antigens / Q000008:administration & dosage* / Q000276:immunology; D002690:Chlamydia Infections / Q000276:immunology / Q000517:prevention & control*; D016993:Chlamydophila pneumoniae / Q000276:immunology*; D004168:Diphtheria Toxoid / Q000008:administration & dosage / Q000276:immunology; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D007114:Immunization; D008297:Male; D023321:Poliovirus Vaccines / Q000008:administration & dosage / Q000276:immunology; D013745:Tetanus Toxoid / Q000008:administration & dosage / Q000276:immunology; D022341:Yellow Fever Vaccine / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000941:Antigens; D004168:Diphtheria Toxoid; D023321:Poliovirus Vaccines; D013745:Tetanus Toxoid; D022341:Yellow Fever Vaccine