title: Quantification of brain mu-opioid receptors with [11C]carfentanil: reference-tissue methods.
abstract: [(11)C]Carfentanil (CFN) is a mu-opioid agonist used for in vivo positron emission tomography (PET) studies of mu-opioid receptors. Previously, a tissue-ratio method was validated for the quantification of CFN binding. However, since that initial validation, several other blood independent (reference-tissue) methods have become available. To evaluate these methods, CFN PET studies with arterial blood sampling were acquired in six healthy male control subjects. Specific binding estimates obtained from reference-tissue methods were compared to those obtained with a more rigorous blood input modeling technique. It was determined that both a graphical method, and a simplified reference tissue model, were more accurate than the tissue-ratio method for quantification of CFN binding.
authors.affiliation: Department of Radiology, Johns Hopkins University, Baltimore, MD 21287, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Endres
authors.forename: Badreddine
authors.initials: B
authors.lastname: Bencherif
authors.forename: John
authors.initials: J
authors.lastname: Hilton
authors.forename: Igal
authors.initials: I
authors.lastname: Madar
authors.forename: J James
authors.initials: JJ
authors.lastname: Frost
mesh_terms: D000328:Adult; D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging / Q000378:metabolism*; D002250:Carbon Radioisotopes / Q000097:blood / Q000493:pharmacokinetics; D003198:Computer Simulation; D003580:Cytochromes; D003576:Electron Transport Complex IV; D005283:Fentanyl / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics*; D006801:Humans; D008297:Male; D051379:Mice; D008954:Models, Biological; D009572:Nitrite Reductases; D011865:Radioisotope Dilution Technique / Q000592:standards; D019275:Radiopharmaceuticals / Q000097:blood / Q000493:pharmacokinetics; D017450:Receptors, Opioid, mu / Q000378:metabolism*; D012015:Reference Standards; D014018:Tissue Distribution; D014055:Tomography, Emission-Computed / Q000379:methods* / Q000592:standards*
chemical_list: D002250:Carbon Radioisotopes; D003580:Cytochromes; D019275:Radiopharmaceuticals; D017450:Receptors, Opioid, mu; D009572:Nitrite Reductases; C028123:nitrite reductase (NO-forming); D003576:Electron Transport Complex IV; C017114:carfentanil; D005283:Fentanyl