Soluble glucocorticoid-induced tumor necrosis factor receptor (sGITR) increased MMP-9 activity in murine macrophage.
Glucocorticoid induced tumor necrosis factor receptor (GITR), a new TNFR family, increased production of matrix matalloproteinase (MMP-9) in murine macrophages. Murine macrophages produced a band of gelatinolytic activity at 100 kDa when stimulated for 18 h with soluble GITR. MMP-9 was identified by gelatin zymography and Western blot. Previous results demonstrated that murine macrophages express GITR and GITR ligand constitutively. Induction of MMP-9 was synergistic with co-treatment of INF-gamma. MMPs could play a critical role in progression and promotion of tissue injury after inflammation stimulated by GITR/ligand system.
Lee
Hee-Sook
HS
Department of Biological Sciences and the Immunomodulation Research Center, University of Ulsan, Ulsan 680-749, Korea.
Shin
Hyun-Hee
HH
Kwon
Byoung S
BS
Choi
Hye-Seon
HS
D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000032:analysis / Q000502:physiology; D004790:Enzyme Induction; D053450:Glucocorticoid-Induced TNFR-Related Protein; D007371:Interferon-gamma / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000096:biosynthesis
D004274:DNA, Recombinant; D053450:Glucocorticoid-Induced TNFR-Related Protein; D017475:Receptors, Nerve Growth Factor; D018124:Receptors, Tumor Necrosis Factor; D011994:Recombinant Proteins; C106544:Tnfrsf18 protein, mouse; D007371:Interferon-gamma; D020780:Matrix Metalloproteinase 9