Semiconductor quantum dot/albumin complex is a long-life and highly photostable endosome marker.
For the purpose of selecting the efficient dispersion condition of hydrophilic semiconductor quantum dots (QDs) in biological buffers, the dispersion of the QDs mixed with a serum albumin from 9 different species or an ovalbumin was compared by a fluorescence intensity analysis. The QDs mixed with sheep serum albumin (SSA) showed the highest fluorescence of all when the mixtures were dissolved in Dulbecco's MEM. QD/SSA complexes were accumulated in the endosome/lysosome of Vero cells and the fluorescence could be detected over a 5-day post-incubation period. The photostability of QD/SSA complexes associated with the endosomes was detectable, at least, 30 times as long as that of fluorescein-labeled dextran involved in endosomes. QD/SSA complex, therefore, can be used as a long-life and highly photostable endosome marker.
Hanaki
Ken-ichi
K
Domestic Research Fellow, Japan Science and Technology Corporation, 4-1-8 Honmachi, Kawaguchi, Saitama 332-0012, Japan. hanaki@ri.imcj.go.jp
Momo
Asami
A
Oku
Taisuke
T
Komoto
Atsushi
A
Maenosono
Shinya
S
Yamaguchi
Yukio
Y
Yamamoto
Kenji
K
D000418:Albumins / Q000737:chemistry*; D000818:Animals; D015415:Biomarkers; D002455:Cell Division; D002522:Chlorocebus aethiops; D011992:Endosomes / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism; D018613:Microscopy, Confocal; D012666:Semiconductors; D012756:Sheep; D013045:Species Specificity; D013053:Spectrophotometry; D013997:Time Factors; D014709:Vero Cells
D000418:Albumins; D015415:Biomarkers