Role of chloroplast protein kinase Stt7 in LHCII phosphorylation and state transition in Chlamydomonas.
Photosynthetic organisms adapt to changes in light quality by redistributing light excitation energy between two photosystems through state transition. This reorganization of antenna systems leads to an enhanced photosynthetic yield. Using a genetic approach in Chlamydomonas reinhardtii to dissect the signal transduction pathway of state transition, we identified a chloroplast thylakoid-associated serine-threonine protein kinase, Stt7, that has homologs in land plants. Stt7 is required for the phosphorylation of the major light-harvesting protein (LHCII) and for state transition.
Depège
Nathalie
N
Department of Molecular Biology and Department of Plant Biology, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva 4, Switzerland.
Bellafiore
Stéphane
S
Rochaix
Jean-David
JD
D020418:Algal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D020134:Catalytic Domain; D016825:Chlamydomonas reinhardtii / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D002736:Chloroplasts / Q000201:enzymology; D003360:Cosmids; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D005453:Fluorescence; D005796:Genes; D005816:Genetic Complementation Test; D008027:Light; D008969:Molecular Sequence Data; D009154:Mutation; D010084:Oxidation-Reduction; D010766:Phosphorylation; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D020524:Thylakoids / Q000201:enzymology*; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D020418:Algal Proteins; D018076:DNA, Complementary; D045322:Photosynthetic Reaction Center Complex Proteins; D017346:Protein Serine-Threonine Kinases