title: Ca2+ ions block and permeate serotonin 5-HT3 receptor channels in rat hippocampal interneurons.
abstract: The serotonin 5-HT(3) receptor native to rat hippocampal CA1 stratum radiatum interneurons is blocked by Ca(2+) ions in a dose- and voltage-dependent manner, which is reflected by a region of negative slope conductance in the I-V curve. The steep dependence on the extracellular Ca(2+) concentration suggests that the channel contains more than one binding site for Ca(2+). A three barrier-two site model, based on Eyring rate theory, was used to describe the I-V curves. When extra- and intracellular K(+) and Cs(+) were substituted with Na(+), the I-V curves were accurately fit by the model, unlike the I-V curves recorded under standard ionic conditions. This suggests that the K(+) and Cs(+) permeabilities are small compared with that of Na(+). The distribution of the energy barriers and binding sites for Ca(2+) and Na(+) showed that the binding sites are located at approximately the 13' and the -4' position in the ion channel. The model predicts that at large hyperpolarized membrane potentials (more negative than -120 mV), the fractional Ca(2+) current amounts to approximately 1% of the total ion current. However, at physiologically relevant membrane potentials, the fractional Ca(2+) current is smaller (<0.1%) and the relative Ca(2+) permeability (P(Ca)/P(Na)) is estimated to be 0.10 at -60 mV.
authors.affiliation: University of Amsterdam, Swammerdam Institute for Life Sciences, Section Neurobiology, NL-1090 GB Amsterdam, The Netherlands. hooft@science.uva.nl
authors.forename: Johannes A
authors.initials: JA
authors.lastname: Van Hooft
authors.forename: Wytse J
authors.initials: WJ
authors.lastname: Wadman
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000502:physiology; D002118:Calcium / Q000494:pharmacology*; D005110:Extracellular Space / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology; D007395:Interneurons / Q000502:physiology*; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008959:Models, Neurological; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D011985:Receptors, Serotonin / Q000737:chemistry / Q000378:metabolism*; D044406:Receptors, Serotonin, 5-HT3
chemical_list: D011985:Receptors, Serotonin; D044406:Receptors, Serotonin, 5-HT3; D002118:Calcium