8-Isoprostaglandin F2a and ascorbic acid concentration in the aqueous humour of patients with exfoliation syndrome.
OBJECTIVE
The authors investigated the concentrations of 8-isoprostaglandin F(2a), a marker of oxidative stress in vivo, and ascorbic acid, a protectant against oxidative damage, in the aqueous humour of patients with exfoliation syndrome (XFS) and cataract and compared the results with those in age matched patients with cataract, but without XFS, to determine whether XFS is associated with increased oxidative stress.


METHODS
Aqueous humour was aspirated at the beginning of phacoemulsification cataract surgery from 27 eyes of 27 cataract patients with XFS and 27 eyes of 27 age matched cataract patients without XFS. 8-Isoprostaglandin F(2a)concentration in the aqueous was determined with a commercial immunoassay; ascorbic acid concentration was measured with a microplate assay method.


RESULTS
The mean concentration of 8-isoprostaglandin F(2a)in the aqueous from patients with XFS (2429 (SD 2940) pg/ml; range 400-10500 pg/ml) was significantly higher than that measured in the aqueous of age matched control patients (529.1 (226.8) pg/ml; range 325-1000 pg/ml); (p = 0.0028). Furthermore, mean ascorbic acid concentration in XFS patients (0.75 (0.39) mM; range 0.28-1.70 mM) was significantly lower than that found in control patients (1.19 (0.47) mM; range 0.53-2.4 mM); (p = 0.0005). There was a reverse correlation between 8-isoprostaglandin F(2a)and ascorbic acid concentration.


CONCLUSIONS
8-Isoprostaglandin F(2a)was significantly increased in the aqueous of patients with XFS, and ascorbic acid was decreased, providing evidence of a role for free radical induced oxidative damage in the pathobiology of XFS.
Koliakos
G G
GG
Department of Biological Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece. koliakos@med.auth.gr
Konstas
A G P
AG
Schlötzer-Schrehardt
U
U
Hollo
G
G
Katsimbris
I E
IE
Georgiadis
N
N
Ritch
R
R
D000975:Antioxidants / Q000032:analysis*; D001082:Aqueous Humor / Q000737:chemistry*; D001205:Ascorbic Acid / Q000032:analysis*; D002386:Cataract / Q000378:metabolism; D015237:Dinoprost / Q000031:analogs & derivatives*; D017889:Exfoliation Syndrome / Q000378:metabolism*; D028441:F2-Isoprostanes / Q000032:analysis*; D016166:Free Radical Scavengers / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D018384:Oxidative Stress; D011446:Prospective Studies
D000975:Antioxidants; D028441:F2-Isoprostanes; D016166:Free Radical Scavengers; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D001205:Ascorbic Acid