Ethanol- and Fe(+2)-induced membrane lipid oxidation is not additive in developing chick brains.
In order to study the effects of exogenous EtOH and/or Fe(+2) on membrane lipid peroxidation, exogenous EtOH, FeCl(2), FeCl(2) & EtOH, NaCl and NaCl & EtOH were injected into fertile chicken eggs. Controls were either shams or injected with saline. These injections were made at 0 days or 0-2 days of development and tissue removed at stage 37 (11 days of development). Embryonic exposure to exogenous EtOH and/or Fe(+2) promoted decreased brain mass, decreased levels of brain membrane polyunsaturated fatty acids, elevated levels of brain lipid hydroperoxides, and elevated levels of Fe(+2) within embryonic brain and liver. These alterations were more severe in triple-injected embryos (E0-2/E11) as compared to single-injected embryos (E0/E11). While exogenous treatments of either EtOH and/or FeCl(2) promoted increased levels of endogenous brain Fe(+2), the effects were not additive. These observations are consistent with the hypothesis that embryonic exposure to exogenous EtOH and/or Fe(+2) promotes brain membrane lipid peroxidation.
Miller
Robert R
RR
Biology Department, Hillsdale College, 33 E College, Hillsdale, MI 49242-1205, USA. bob.miller@hillsdale.edu
Coughlin
Daniel J
DJ
Fraser-Thomson
Elisabeth Sabrina
ES
Noe
Elizabeth C
EC
Palenick
Amanda
A
Voorhees
Emily B
EB
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D002642:Chick Embryo; D004338:Drug Combinations; D000431:Ethanol / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D005296:Ferrous Compounds / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000737:chemistry / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects
D004338:Drug Combinations; D005231:Fatty Acids, Unsaturated; D005296:Ferrous Compounds; D000431:Ethanol