title: Molecular size distribution analysis of human gingival glycosaminoglycans in cyclosporin- and nifedipine-induced overgrowths.
abstract: Glycosaminoglycans are thought to accumulate in formative lesions like drug-induced gingival overgrowth. Recent evidences, however, suggest that the amounts of glycosaminoglycans are comparable in overgrown and healthy gingiva. Besides, alterations in the size distribution of glycosaminoglycan molecules isolated from phenytoin-induced overgrown samples have also been suggested. Therefore, we sought to determine possible differences in molecular size distribution of gingival glycosaminoglycans in other types of drug-induced overgrowths. Purified gingival glycosaminoglycans from healthy and cyclosporin- and nifedipine-induced overgrown gingival tissues were analyzed by agarose gel electrophoresis and their molecular-size distribution was evaluated by both gel filtration chromatography and polyacrylamide gel electrophoresis. Our results on the gingival glycosaminoglycan composition showed presence of chondroitin sulfate, dermatan sulfate, heparan sulfate and hyaluronic acid in all types of gingival tissues examined. In addition, hyaluronic acid was predominantly of a large size eluting near to the void volume of a Superose-6 column, while the sulfated glycosaminoglycans were mainly composed of low molecular size glycosaminoglycans. Our results show no differences in the molecular-size distribution of hyaluronic acid and sulfated glycosaminoglycans among healthy and drug-induced overgrown gingival tissues.
authors.affiliation: Laboratório de Tecido Conjuntivo, Hospital Universitário Clementino Fraga Filho, Brasil.
authors.forename: Rita C L
authors.initials: RC
authors.lastname: Martins
authors.forename: Claudio C
authors.initials: CC
authors.lastname: Werneck
authors.forename: Lia A G
authors.initials: LA
authors.lastname: Rocha
authors.forename: Eduardo J
authors.initials: EJ
authors.lastname: Feres-Filho
authors.forename: Luiz-Claudio F
authors.initials: LC
authors.lastname: Silva
mesh_terms: D000293:Adolescent; D000328:Adult; D002121:Calcium Channel Blockers / Q000009:adverse effects*; D002809:Chondroitin Sulfates / Q000302:isolation & purification; D002850:Chromatography, Gel; D016572:Cyclosporine / Q000009:adverse effects*; D003871:Dermatan Sulfate / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005881:Gingiva / Q000737:chemistry*; D019214:Gingival Overgrowth / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000737:chemistry* / Q000302:isolation & purification; D006497:Heparan Sulfate / Q000302:isolation & purification; D006801:Humans; D006820:Hyaluronic Acid / Q000302:isolation & purification; D007166:Immunosuppressive Agents / Q000009:adverse effects*; D008875:Middle Aged; D015394:Molecular Structure; D008970:Molecular Weight; D009543:Nifedipine / Q000009:adverse effects*; D012685:Sepharose
chemical_list: D002121:Calcium Channel Blockers; D002809:Chondroitin Sulfates; D016572:Cyclosporine; D003871:Dermatan Sulfate; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006820:Hyaluronic Acid; D007166:Immunosuppressive Agents; D009543:Nifedipine; D012685:Sepharose; C043127:Superose 6b