Trypsinogen hL expressed in the human lung is a new member of the trypsinogen family.
Molecular cloning of cDNA encoding a new member of the trypsinogen family, named trypsinogen hL, was carried out by PCR using human lung cDNAs as templates. The primary structure of trypsinogen hL was found to be a prepro-protein and a catalytic triad, 64His, 108Asp and 201Ser. It was also found that trypsinogen hL is specifically expressed in the human lung, the expression level being 30-times higher than those in other tissues tested. A phylogenic tree analysis showed that trypsinogen hL is a new member of the trypsinogen family, a family of serine protease family proteins.
Imamura
Yasuhiro
Y
Division of Molecular Biology, Research Institute for Biological Sciences, Tokyo University of Science, Noda, Chiba, Japan.
Katsu
Masataka
M
Sakai
Kaoru
K
Okumura
Yuushi
Y
Ariga
Hiroyoshi
H
Kido
Hiroshi
H
D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D019143:Evolution, Molecular; D015870:Gene Expression / Q000502:physiology*; D006801:Humans; D008168:Lung / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment / Q000379:methods; D017385:Sequence Homology; D012697:Serine Endopeptidases / Q000145:classification / Q000235:genetics; D014362:Trypsinogen / Q000145:classification / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D012333:RNA, Messenger; C477927:Trypsinogen hL , human; D014362:Trypsinogen; D012697:Serine Endopeptidases