Loss of cables protein expression in human non-small cell lung cancer: a tissue microarray study.
Loss of heterozygosity (LOH) on chromosome 18q is common in lung cancer. The genes involved in LOH on 18q in lung cancer have not been well characterized. Cables, a cyclin-dependent kinase (cdk) interacting protein, has recently been identified and mapped to human chromosome 18q11-12. Cables inhibits cell growth and suppresses tumor formation in nude mice, making it a candidate gene for 18q LOH in lung cancer. Little is known regarding Cables protein expression in human non-small cell lung cancer (NSCLC). In this study we examined Cables expression in 163 NSCLC and nonneoplastic lung specimens using tissue microarrays. Strong nuclear staining was present in normal lung and bronchial tissue. We also evaluated the Cables protein expression pattern and its correlation with histopathologic features and with clinical course of NSCLC. The results of the present study demonstrate for the first time that numerous NSCLCs (45%) lose Cables expression. Furthermore, more adenocarcinomas show a loss of this novel protein than do squamous counterparts. The relationship between tumor histology type and Cables expression appears to be statistically significant (P = 0.028). Our results suggest that Cables may be involved in the pathogenesis of NSCLC.
Tan
Dongfeng
D
Department of Pathology, Roswell Park Cancer Institute, State University of New York, Buffalo 14263, USA.
Kirley
Sandy
S
Li
Qiang
Q
Ramnath
Nithya
N
Slocum
Harry K
HK
Brooks
John S
JS
Wu
Chin-Lee
CL
Zukerberg
Lawrence R
LR
D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000473:pathology; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D002887:Chromosomes, Human, Pair 18; D016213:Cyclins; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D019656:Loss of Heterozygosity; D008175:Lung Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010750:Phosphoproteins / Q000032:analysis* / Q000235:genetics; D012189:Retrospective Studies; D015996:Survival Rate
C486568:CABLES1 protein, human; D002352:Carrier Proteins; D016213:Cyclins; D010750:Phosphoproteins