Isolation of cytomegalovirus and foamy virus from the drill monkey (Mandrillus leucophaeus) and prevalence of antibodies to these viruses amongst wild-born and captive-bred individuals.
Drill monkeys (Mandrillus leucophaeus) are an endangered species whose indigenous viral flora is largely unknown. We report here the isolation and characterization of both a cytomegalovirus (DrCMV) and a foamy virus (SFV-drl) from drill monkeys. Phylogenetic analysis of DNA sequence data placed the DrCMV within a primate CMV clade, and showed that SFV-drl was closely related to baboon foamy viruses. ELISA analysis demonstrated that DrCMV shared common epitopes with other primate CMVs but was distinct from human and baboon CMVs. ELISA testing of sera from wild-born and captive-bred drills indicated that the prevalence of antibodies reactive with these two viruses was 93.2% for DrCMV and 20.3% for SFV-drl.
Blewett
E L
EL
Department of Biochemistry and Microbiology, College of Osteopathic Medicine, Oklahoma State University, Tulsa, Oklahoma 74107, U.S.A. micro@earlblewett.net
Lewis
J
J
Gadsby
E L
EL
Neubauer
S R
SR
Eberle
R
R
D000818:Animals; D000835:Animals, Wild; D000836:Animals, Zoo; D000914:Antibodies, Viral / Q000097:blood*; D003587:Cytomegalovirus / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000662:veterinary / Q000821:virology; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000821:virology*; D010215:Papio / Q000821:virology*; D012192:Retroviridae Infections / Q000662:veterinary / Q000821:virology; D017422:Sequence Analysis, DNA; D016092:Spumavirus / Q000276:immunology / Q000302:isolation & purification*; D014759:Viral Envelope Proteins / Q000235:genetics
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; D004259:DNA-Directed DNA Polymerase