title: Construction and evaluation of internal control DNA for PCR amplification of Chlamydia trachomatis DNA from urine samples.
abstract: An internal control DNA (ICD) with the same primer binding sequences as the target Chlamydia trachomatis DNA was constructed and evaluated in a PCR assay with immunoenzymatic detection. One hundred urine specimens were tested, and 23 were found to contain inhibitors of the PCR, if not subjected to DNA extraction prior to amplification. Coamplification and detection of the ICD appeared to be a useful method for estimating the effects of inhibitors on C. trachomatis DNA amplification.
authors.affiliation: Biobanque de Picardie, 80000 Amiens, France. fay.betsou@biobanque-picaridie.com
authors.forename: Fotini
authors.initials: F
authors.lastname: Betsou
authors.forename: Katy
authors.initials: K
authors.lastname: Beaumont
authors.forename: Jean Marie
authors.initials: JM
authors.lastname: Sueur
authors.forename: Jeanne
authors.initials: J
authors.lastname: Orfila
mesh_terms: D015373:Bacterial Typing Techniques; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis*; D005784:Gene Amplification; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011786:Quality Control; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D014556:Urine / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic