Asymmetric inheritance of oxidatively damaged proteins during cytokinesis.
Carbonylated proteins were visualized in single cells of the budding yeast Saccharomyces cerevisiae, revealing that they accumulate with replicative age. Furthermore, carbonylated proteins were not inherited by daughter cells during cytokinesis. Mother cells of a yeast strain lacking the sir2 gene, a life-span determinant, failed to retain oxidatively damaged proteins during cytokinesis. These findings suggest that a genetically determined, Sir2p-dependent asymmetric inheritance of oxidatively damaged proteins may contribute to free-radical defense and the fitness of newborn cells.
Aguilaniu
Hugo
H
Department of Cell and Molecular Biology-Microbiology, Göteborg University, Göteborg, Sweden.
Gustafsson
Lena
L
Rigoulet
Michel
M
Nyström
Thomas
T
D000199:Actins / Q000378:metabolism; D002455:Cell Division; D005800:Genes, Fungal; D006655:Histone Deacetylases / Q000235:genetics / Q000502:physiology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007150:Immunohistochemistry; D008928:Mitochondria / Q000378:metabolism; D009154:Mutation; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010269:Paraquat / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology; D056565:Sirtuin 2; D037761:Sirtuins / Q000235:genetics / Q000502:physiology; D013481:Superoxides / Q000378:metabolism
D000199:Actins; D017382:Reactive Oxygen Species; C084122:SIR3 protein, S cerevisiae; C061573:SIR4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; D013481:Superoxides; D006861:Hydrogen Peroxide; C087778:SIR2 protein, S cerevisiae; D056565:Sirtuin 2; D037761:Sirtuins; D006655:Histone Deacetylases; D010269:Paraquat