title: Nevirapine resistance mutation at codon 181 of the HIV-1 reverse transcriptase confers stavudine resistance by increasing nucleotide substrate discrimination and phosphorolytic activity.
abstract: Recombinant HIV-1 reverse transcriptase (RT) carrying non-nucleoside inhibitors (NNRTIs) resistance mutation at codon 181 showed reduced incorporation and high efficiency of phosphorolytic removal of stavudine, a nucleoside RT inhibitor. These results reveal a new mechanism for cross-resistance between different classes of HIV-1 RT inhibitors.
authors.affiliation: Istituto di Genetica Molecolare, Consiglio Nazionale delle Ricerche, via Abbiategrasso 207, I-27100 Pavia, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Blanca
authors.forename: Fausto
authors.initials: F
authors.lastname: Baldanti
authors.forename: Stefania
authors.initials: S
authors.lastname: Paolucci
authors.forename: Alexander Yu
authors.initials: AY
authors.lastname: Skoblov
authors.forename: Lyubov
authors.initials: L
authors.lastname: Victorova
authors.forename: Ulrich
authors.initials: U
authors.lastname: Hübscher
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Gerna
authors.forename: Silvio
authors.initials: S
authors.lastname: Spadari
authors.forename: Giovanni
authors.initials: G
authors.lastname: Maga
mesh_terms: D003062:Codon; D024882:Drug Resistance, Viral; D054303:HIV Reverse Transcriptase / Q000235:genetics*; D009154:Mutation; D019829:Nevirapine / Q000494:pharmacology*; D009711:Nucleotides / Q000378:metabolism; D010766:Phosphorylation; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology*; D018119:Stavudine / Q000494:pharmacology*; D015215:Zidovudine / Q000494:pharmacology
chemical_list: D003062:Codon; D009711:Nucleotides; D018894:Reverse Transcriptase Inhibitors; D015215:Zidovudine; D019829:Nevirapine; D018119:Stavudine; D054303:HIV Reverse Transcriptase