Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants.
To characterize ammonium transport pathways in rice, two cDNAs with high homology to MEP/AMT2-type ammonium transporters, OsAMT2;1 and OsAMT3;1, were isolated. Expression of OsAMT2;1 in an ammonium-uptake-defective yeast mutant showed that this gene encodes functional ammonium transporters. OsAMT2;1 was constitutively expressed in both roots and shoots irrespective of the supply of inorganic nitrogen to the medium, whereas OsAMT3;1 expression was relatively weak. A database search with the amino acid sequence of OsAMT2;1 showed that there are 10 putative OsAMT genes in rice, i.e. three each for OsAMT1, OsAMT2 and OsAMT3, respectively, and one for OsAMT4.
Suenaga
Arata
A
Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555 Japan.
Moriya
Kanami
K
Sonoda
Yutaka
Y
Ikeda
Akira
A
Von Wirén
Nicolaus
N
Hayakawa
Toshihiko
T
Yamaguchi
Junji
J
Yamaya
Tomoyuki
T
D000643:Ammonium Chloride / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics*; D027682:Cation Transport Proteins; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009566:Nitrates / Q000494:pharmacology; D012275:Oryza / Q000187:drug effects / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017680:Potassium Compounds / Q000494:pharmacology; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
D002352:Carrier Proteins; D027682:Cation Transport Proteins; D009566:Nitrates; D010940:Plant Proteins; D017680:Potassium Compounds; D012333:RNA, Messenger; C088952:ammonium transporters, plant; D000643:Ammonium Chloride; C023844:potassium nitrate