[Expression of a new lung cancer drug resistance-related gene in lung cancer tissues and lung cancer cell strains].
OBJECTIVE
A new drug resistance-related gene fragment which was 494 bp long was found using suppression subtractive hybridization (SSH) and its full-length cDNA fragment was cloned by the authors. This study was designed to determine the expression of this lung cancer drug resistance-related gene (LCDRG) in lung cancer tissues, juxtacancerous tissues, and five lung cancer cell strains.


METHODS
The expression of LCDRG was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) method in 38 lung cancer tissues,12 juxtacancerous tissues, and 5 lung cancer cell strains.


RESULTS
The expression of LCDRG in cancer tissues was significantly higher than that in juxtacancerous tissue (P< 0.001). There was no significance of the expression among adenocarcinoma, brochioloalveolar carcinoma, and squamous carcinoma. In lung cancer cell strains, the expression levels of LCDRG in adenocarcinoma cell strains SPC-A-1 and A549, big cell lung cancer cell strain H460, small cell lung cancer cell strains H446 and SH77 were decreased gradually.


CONCLUSIONS
LCDRG is closely related to lung cancer and may be involved in the pathogenesis of lung cancer.
Liu
Ling-Zhi
LZ
Institute of Respiratory Disease, Xinqiao Hospital, The Third Military Medical University, Chongqing, 400037, PR China.
Qian
Gui-Sheng
GS
Zhou
Xiang-Dong
XD
D000328:Adult; D000368:Aged; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction
D009363:Neoplasm Proteins