title: Development of PCR assays for detection of Streptococcus canis.
abstract: Streptococcus canis isolates, also including S. canis of artificially contaminated milk, could be identified by polymerase chain reaction (PCR) amplification using oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene and, after sequencing, according to S. canis-specific parts of the 16S-23S rDNA intergenic spacer region and with oligonucleotide primers detecting an internal fragment of the group G streptococcal CAMP factor gene cfg. The 16S rRNA gene- and CAMP factor gene cfg-specific oligonucleotide primers could be used together in a multiplex PCR. No cross-reactivities could be observed with other group G streptococcal isolates or with any of the other control strains of various streptococcal species and serogroups. The PCR methods presented in this study allowed a rapid and reliable identification of S. canis and might help to improve the diagnosis of this bacterial species in animal and human infections.
authors.affiliation: Institut für Tierärztliche Nahrungsmittelkunde, Professur für Milchwissenschaften, Justus-Liebig-Universität Giessen, Ludwig Str. 21, 35390, Giessen, Germany.
authors.forename: A A
authors.initials: AA
authors.lastname: Hassan
authors.forename: I U
authors.initials: IU
authors.lastname: Khan
authors.forename: A
authors.initials: A
authors.lastname: Abdulmawjood
authors.forename: C
authors.initials: C
authors.lastname: Lämmler
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002417:Cattle; D021901:DNA, Intergenic; D004285:Dogs; D006460:Hemolysin Proteins; D008892:Milk / Q000382:microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S; D016415:Sequence Alignment; D013045:Species Specificity; D013291:Streptococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; C039092:CAMP protein, Streptococcus; D021901:DNA, Intergenic; D006460:Hemolysin Proteins; D012336:RNA, Ribosomal, 16S