title: Entamoeba histolytica: acute granulomatous intestinal lesions in normal and neutrophil-depleted mice.
abstract: To study the role of neutrophils in the innate resistance to Entamoeba histolytica intestinal infection in mice, animals were treated with anti-neutrophil monoclonal antibodies prior to intracecal parasite inoculation and the resulting lesions were compared with normal mice that had been equally infected. In contrast to our previous finding that neutrophils are critical in eliminating E. histolytica infection in the liver, we show here that neutrophils are not absolutely required to eliminate E. histolytica infection from the intestine. Although the neutrophils are not critical for resolution of the E. histolytica infection, neutrophils do appear to provide some measure of protection as the intestinal amoeba burden was higher at early timepoints after infection in the neutropenic animals. In addition, we found that while both the normal and the neutrophil-depleted mice developed ulcerative lesions in the colon, the neutropenic mice had an increased frequency of granulomas that formed around the amoeba. Thus, our findings appear to be the first evidence showing that granulomatous inflammation can occur after intestinal infection in mice using axenically cultured amoeba.
authors.affiliation: Department of Cell Biology, Centro de Investigación y Estudios Avanzados (CINVESTAV), Instituto Politécnico Nacional, Av. IPN 2508, Col. San Pedro Zacatenco, DF, Mexico.
authors.forename: Laura
authors.initials: L
authors.lastname: Rivero-Nava
authors.forename: Jesús
authors.initials: J
authors.lastname: Aguirre-García
authors.forename: Mineko
authors.initials: M
authors.lastname: Shibayama-Salas
authors.forename: Rogelio
authors.initials: R
authors.lastname: Hernández-Pando
authors.forename: Víctor
authors.initials: V
authors.lastname: Tsutsumi
authors.forename: Jesús
authors.initials: J
authors.lastname: Calderón
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002432:Cecum / Q000469:parasitology; D004404:Dysentery, Amebic / Q000276:immunology* / Q000473:pathology; D004748:Entamoeba histolytica / Q000276:immunology* / Q000502:physiology; D005260:Female; D006099:Granuloma / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D007074:Immunoglobulin G / Q000276:immunology; D007422:Intestines / Q000276:immunology / Q000469:parasitology / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009503:Neutropenia / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D009504:Neutrophils / Q000276:immunology*; D014456:Ulcer / Q000276:immunology / Q000469:parasitology / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G