Genome-wide screening for complete genetic loss in prostate cancer by comparative hybridization onto cDNA microarrays.
We demonstrate that comparative genomic hybridization (CGH) onto cDNA microarrays may be used to carry out genome-wide screens for regions of genetic loss, including homozygous (complete) deletions that may represent the possible location of tumour suppressor genes in human cancer. Screening of the prostate cancer cell lines LNCaP, PC3 and DU145 allowed the mapping of specific regions where genome copy number appeared altered and led to the identification of two novel regions of complete loss at 17q21.31 (500 kb spanning STAT3) and at 10q23.1 (50-350 kb spanning SFTPA2) in the PC3 cell line.
Clark
Jeremy
J
Molecular Carcinogenesis Section, Male Urological Cancer Research Center, Institute of Cancer Research, Sutton, Surrey, UK. csc@icr.ac.uk
Edwards
Sandra
S
Feber
Andrew
A
Flohr
Penny
P
John
Megan
M
Giddings
Ian
I
Crossland
Sue
S
Stratton
Michael R
MR
Wooster
Richard
R
Campbell
Colin
C
Cooper
Colin S
CS
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D017353:Gene Deletion; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000737:chemistry
D018076:DNA, Complementary; D004273:DNA, Neoplasm