Suppression subtractive hybridization identifies distinctive expression markers for coronary and internal mammary arteries.
OBJECTIVE
We sought to identify differentially expressed genes in the athero-prone coronary artery and athero-resistant internal mammary arteries.


RESULTS
Using suppressive subtraction hybridization, we generated reciprocal cDNA collections of representative mRNAs specific to porcine coronary arteries versus porcine mammary arteries. We screened 1000 suppressive subtraction hybridization cDNA clones by dot blot array and sequenced 600 of those showing the most marked expression differences. Northern blot, in situ hybridization, and immunostaining confirmed the differential gene expression patterns identified by the dot blot arrays. Genes associated with mammary arteries included claudin-10 and h-cadherin, which are genes associated with tight junctions and intermediate junctions. In contrast, genes associated with proatherosclerotic processes, such as lipid retention and metabolism, inflammation, and cell growth, were preferentially expressed in coronary arteries.


CONCLUSIONS
Normal coronary arteries have gene expression program that is significantly different than internal mammary arteries. These differences may partly explain the resistance of coronary arteries and internal mammary arteries to atherosclerosis.
Qin
Minghui
M
Atherosclerosis Research Center, Division of Cardiology, and Burns and Allen Research Institute, Cedars-Sinai Medical Center, Davis Bldg #1016, 8700 Beverly Blvd, Los Angeles, Calif 90048, USA.
Zeng
Zhaohui
Z
Zheng
Jie
J
Shah
Prediman K
PK
Schwartz
Stephen M
SM
Adams
Lawrence D
LD
Sharifi
Behrooz G
BG
D000818:Animals; D001161:Arteriosclerosis / Q000235:genetics; D015152:Blotting, Northern; D015820:Cadherins / Q000032:analysis / Q000235:genetics; D051242:Caveolin 1; D022461:Caveolins / Q000032:analysis / Q000235:genetics; D057167:Claudins; D003331:Coronary Vessels / Q000378:metabolism*; D015342:DNA Probes; D018076:DNA, Complementary / Q000032:analysis*; D015870:Gene Expression; D015151:Immunoblotting; D066298:In Vitro Techniques; D008323:Mammary Arteries / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D012016:Reference Values; D012259:Ribonuclease, Pancreatic / Q000032:analysis / Q000235:genetics; D013552:Swine; D019063:Tenascin / Q000032:analysis / Q000235:genetics
D015820:Cadherins; D051242:Caveolin 1; D022461:Caveolins; D057167:Claudins; D015342:DNA Probes; D018076:DNA, Complementary; C099972:H-cadherin; D008565:Membrane Proteins; D019063:Tenascin; C502009:claudin 10; C046366:angiogenin; D012259:Ribonuclease, Pancreatic