The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi.
In this review we will focus on the current status and views concerning the production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. We will focus on single-chain antibody fragment production (scFv and VHH) by these lower eukaryotes and the possible applications of these proteins. Also the coupling of fragments to relevant enzymes or other components will be discussed. As an example of the fusion protein strategy, the 'magic bullet' approach for industrial applications, will be highlighted.
Joosten
Vivi
V
TNO Nutrition and Food Research, Department of Applied Microbiology and Gene Technology, P,O, Box 360, 3700 AJ Zeist, The Netherlands. joosten@voeding.tno.nl
Lokman
Christien
C
Van Den Hondel
Cees AMJJ
CA
Punt
Peter J
PJ