The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells.
The RNA helicases p68 and p72 are highly related members of the DEAD box family of proteins, sharing 90% identity across the conserved core, and have been shown to be involved in both transcription and mRNA processing. We previously showed that these proteins co-localise in the nucleus of interphase cells. In this study we show that p68 and p72 can interact with each other and self-associate in the yeast two-hybrid system. Co-immunoprecipitation experiments confirmed that p68 and p72 can interact in the cell and indicated that these proteins preferentially exist as hetero-dimers. In addition, we show that p68 can interact with NFAR-2, a protein that is also thought to function in mRNA processing. Moreover, gel filtration analysis suggests that p68 and p72 can exist in a variety of complexes in the cell (ranging from approximately 150 to approximately 400 kDa in size), with a subset of p68 molecules being in very large complexes (>2 MDa). The potential to exist in different complexes that may contain p68 and/or p72, together with a range of other factors, would provide the potential for these proteins to interact with different RNA substrates and would be consistent with recent reports implying a wide range of functions for p68/p72.
Ogilvie
V C
VC
Department of Molecular and Cellular Pathology, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK.
Wilson
B J
BJ
Nicol
S M
SM
Morrice
N A
NA
Saunders
L R
LR
Barber
G N
GN
Fuller-Pace
F V
FV
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001667:Binding, Competitive; D002460:Cell Line; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D053487:DEAD-box RNA Helicases; D019281:Dimerization; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D050779:Nuclear Factor 90 Proteins; D010750:Phosphoproteins; D011233:Precipitin Tests; D011485:Protein Binding; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020365:RNA Helicases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D020798:Two-Hybrid System Techniques
D002868:Chromosomal Proteins, Non-Histone; C429643:ILF3 protein, human; D050779:Nuclear Factor 90 Proteins; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; C047458:fibrillarin; D011494:Protein Kinases; D000251:Adenosine Triphosphatases; C102004:DDX17 protein, human; C506749:Ddx5 protein, human; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases