title: The power and prospects of fluorescence microscopies and spectroscopies.
abstract: Recent years have witnessed a renaissance of fluorescence microscopy techniques and applications, from live-animal multiphoton confocal microscopy to single-molecule fluorescence spectroscopy and imaging in living cells. These achievements have been made possible not so much because of improvements in microscope design, but rather because of development of new detectors, accessible continuous wave and pulsed laser sources, sophisticated multiparameter analysis on one hand, and the development of new probes and labeling chemistries on the other. This review tracks the lineage of ideas and the evolution of thinking that have led to the actual developments, and presents a comprehensive overview of the field, with emphasis put on our laboratory's interest in single-molecule microscopy and spectroscopy.
authors.affiliation: Department of Chemistry and Biochemistry, UCLA, Young Hall, 607 Charles E. Young Drive East, Los Angeles, California 90095, USA. michalet@chem.ucla.edu
authors.forename: Xavier
authors.initials: X
authors.lastname: Michalet
authors.forename: Achillefs N
authors.initials: AN
authors.lastname: Kapanidis
authors.forename: Ted
authors.initials: T
authors.lastname: Laurence
authors.forename: Fabien
authors.initials: F
authors.lastname: Pinaud
authors.forename: Soeren
authors.initials: S
authors.lastname: Doose
authors.forename: Malte
authors.initials: M
authors.lastname: Pflughoefft
authors.forename: Shimon
authors.initials: S
authors.lastname: Weiss
mesh_terms: D004867:Equipment Design; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D013194:Staining and Labeling / Q000295:instrumentation / Q000379:methods
chemical_list: D005456:Fluorescent Dyes