Significant improvement of the recombinant Borrelia-specific immunoglobulin G immunoblot test by addition of VlsE and a DbpA homologue derived from Borrelia garinii for diagnosis of early neuroborreliosis.
We investigated whether the recombinant Borrelia Western blot test previously described (B. Wilske, C. Habermann, V. Fingerle, B. Hillenbrand, S. Jauris-Heipke, G. Lehnert, I. Pradel, D. Rössler, and U. Schulte-Spechtel, Med. Microbiol. Immunol. 188:139-144, 1999) can be improved by the addition of VlsE and additional DbpA and OspC homologues. By using a panel of sera from 36 neuroborreliosis patients and 67 control patients, the diagnostic sensitivity of the recombinant immunoblot test was significantly increased (86.1% versus 52.7%) without loss of specificity and was higher (86.1% versus 63.8%) than that of the conventional whole-cell lysate immunoblot test (U. Hauser, G. Lehnert, R. Lobentanzer, and B. Wilske, J. Clin. Microbiol. 35:1433-1444, 1997). Improvement was mainly due to the presence of VlsE and DbpA.
Schulte-Spechtel
Ulrike
U
Max von Pettenkofer-Institut für Medizinische Mikrobiologie und Hygiene der Ludwig-Maximilians-Universität München, D-80336 Munich, Germany. Bettina.Wilske@mvp-bak.med.uni-muenchen.de
Lehnert
Gisela
G
Liegl
Gaby
G
Fingerle
Volker
V
Heimerl
Christiane
C
Johnson
Barbara J B
BJ
Wilske
Bettina
B
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology; D015373:Bacterial Typing Techniques; D015748:Borrelia burgdorferi Group / Q000276:immunology / Q000302:isolation & purification*; D053487:DEAD-box RNA Helicases; D019937:Diagnostic Techniques and Procedures; D029968:Escherichia coli Proteins; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007074:Immunoglobulin G; D008074:Lipoproteins / Q000737:chemistry / Q000276:immunology; D020852:Lyme Neuroborreliosis / Q000175:diagnosis* / Q000382:microbiology; D020365:RNA Helicases / Q000737:chemistry / Q000276:immunology; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D007074:Immunoglobulin G; D008074:Lipoproteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; C105546:VlsE protein, Borrelia burgdorferi; C065908:dbpA protein, E coli; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases