Carbaryl-induced alterations in biochemical metabolism of the prawn, Macrobrachium malcolmsonii.
The present study was performed to investigate the toxic impact of carbaryl on biochemical metabolism in the hemolymph, brain, hepatopancreas, gills and muscle of intermoult juveniles of the economically important prawn, Macrobrachium malcolmsonii. The concentration of glutathione S-transferase (GST) was found to be higher in test prawns when compared with controls. This suggests that a mechanism of detoxification was in operation to neutralise carbaryl toxicity. However, the toxic effect of carbaryl was not fully neutralised, and hence, alterations were recorded in basic biochemical metabolism of test prawns. The concentration of acetylcholinestrase (AchE) was found to be lower in test prawns than that of controls. Carbaryl toxicity resulted in utilisation of major biochemical constituents, such as total carbohydrate, glycogen, protein and lipid to generate required energy as an attempt to withstand the toxic stress. Glycogenolysis resulted in elevation of total free sugar level in the hemolymph of test prawns. While proteolysis led to elevation of total free amino acid level in test prawns. The content of total lipid have also been found lower in test prawns than that of controls. This suggests that carbaryl toxicity resulted in severe energy crises in test prawns. In the present study, toxic effects of carbaryl impair basic metabolic functions and hence pose a threat to the life of M. malcolmsonii.
Bhavan
P Saravana
PS
Department of Zoology, Government Arts College, Dharmapuri-636 705, India.
Geraldine
P
P
D000110:Acetylcholinesterase / Q000032:analysis / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000502:physiology; D012721:Carbaryl / Q000009:adverse effects*; D005880:Gills / Q000502:physiology; D005982:Glutathione Transferase / Q000032:analysis / Q000187:drug effects / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism; D007306:Insecticides / Q000009:adverse effects*; D050356:Lipid Metabolism; D033541:Palaemonidae / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
D000596:Amino Acids; D007306:Insecticides; D014874:Water Pollutants, Chemical; D006003:Glycogen; D005982:Glutathione Transferase; D000110:Acetylcholinesterase; D012721:Carbaryl