Purification of fully activated Clostridium botulinum serotype B toxin for treatment of patients with dystonia.
Clostridium botulinum serotype B toxins 12S and 16S were separated by using a beta-lactose gel column at pH 6.0; toxin 12S passed through the column, whereas toxin 16S bound to the column and eluted with lactose. The fully activated neurotoxin was obtained by applying the trypsin-treated 16S toxin on the same column at pH 8.0; the neurotoxin passed through the column, whereas remaining nontoxic components bound to the column. The toxicity of this purified fully activated neurotoxin was retained for a long period by addition of albumin in the preparation.
Arimitsu
Hideyuki
H
Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan.
Inoue
Kaoru
K
Sakaguchi
Yoshihiko
Y
Lee
Jaechul
J
Fujinaga
Yukako
Y
Watanabe
Toshihiro
T
Ohyama
Tohru
T
Hirst
Robert
R
Oguma
Keiji
K
D000595:Amino Acid Sequence; D001905:Botulinum Toxins / Q000737:chemistry / Q000302:isolation & purification* / Q000627:therapeutic use; D002850:Chromatography, Gel; D004421:Dystonia / Q000188:drug therapy*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data
D001905:Botulinum Toxins