The effects of periurethral muscle-derived stem cell injection on leak point pressure in a rat model of stress urinary incontinence.
Our goal was to determine whether periurethral injection of allogenic muscle-derived stem cells (MDSC) could increase the leak point pressure (LPP) in a denervated female rat model of stress urinary incontinence. Cells isolated from the gastrocnemius muscle of normal female rats were purified for a myogenic population by the preplate technique. Three experimental groups were established: a control group (C) had a sham operation without injections; a sciatic nerve transection group (D) had periurethral saline injections; and a sciatic nerve transsection group had periurethral MDSC injections (M). One week following treatment the LPP of groups C, D and M were 25.2+/-1.9 cmH(2)O, 28.6+/-0.8 cmH(2)O and 36.7+/-2.3 cmH(2)O, respectively. At 4 weeks the LPP of groups C, D and M were 25.8+/-2.5 cmH(2)O, 18.6+/-5.2 cmH(2)O and 44.1+/-6.6 cmH(2)O, respectively. Allogenic MDSC significantly improved the LPP in sciatic nerve-transected animals after both 1 and 4 weeks compared to denervated animals injected with saline.
Lee
J Y
JY
University of Pittsburgh School of Medicine, Suite 700 Kaufmann Building, 3471 Fifth Avenue, Pittsburgh, PA 15213, USA.
Cannon
T W
TW
Pruchnic
R
R
Fraser
M O
MO
Huard
J
J
Chancellor
M B
MB
D000818:Animals; D003714:Denervation; D005260:Female; D018482:Muscle, Skeletal / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve; D033581:Stem Cell Transplantation; D023822:Tissue Engineering; D014550:Urinary Incontinence, Stress / Q000517:prevention & control*