[The significance of nuclear factor kappa Bp65 (NF kappa Bp65) expression on the vascular endothelial cells of rectum adenocarcinoma of human].
OBJECTIVE
This study inquired into the mechanism of cancer vascular metastasis.


METHODS
The immunohistochemical method was adopted in determining the expression of intercellular adhesion molecule-1 (ICAM-1) and nuclear factor kappa B (NF kappa Bp65) on the peritumoral rectum tissues and metastatic lymph nodes of 8 patients and on the rectum tissues and lymph nodes of 5 normal human subjects. Also, the method of in situ hybridization was employed in detecting the binding site of NF kappa Bp65 on vascular endothelial cells.


RESULTS
It was found that the proteins of ICAM-1 and NF kappa Bp65 were expressed on the vascular endothelial cells of the rectum adenocarcinoma patients, and there was a NF kappa B binding consensus sequences on ICAM-1 promoter in the vascular endothelial cells of the rectum adenocarcinoma patients. When DIG-AKP labeled 38-bp oligonucleotide probe was used, there was no expression of ICAM-1 and NF kappa Bp65 on the vascular endothelial cells of the normal human lymph node and rectum tissues.


CONCLUSIONS
The above data suggest that the activation of ICAM-1 promoter may critically depend on NF kappa Bp65 homodimers or heterodimers binding to a variant kappa B site on the vascular endothelial cells of human rectum adenocarcinoma. These may indicate the potential roles of NF kappa B in cancer metastasis, thus giving clues to the development of a novel anti-metastasis strategy.
Chen
Y
Y
Department of Anatomy, School of Basic Medical Sciences, WCUMS, Chengdu 610041, China.
Li
R
R
Wang
R
R
Liu
Z
Z
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D016328:NF-kappa B / Q000096:biosynthesis*; D009362:Neoplasm Metastasis; D012004:Rectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D051996:Transcription Factor RelA
D016328:NF-kappa B; D051996:Transcription Factor RelA; D018799:Intercellular Adhesion Molecule-1