The effects of hyaluronan and its fragments on lipid models exposed to UV irradiation.
The effects of hyaluronan and its degradation products on irradiation-induced lipid peroxidation were investigated. Liposomal skin lipid models with increasing complexity were used. Hyaluronan and its fragments were able to reduce the amount of lipid peroxidation secondary products quantified by the thiobarbituric acid (TBA) assay. The qualitative changes were studied by mass spectrometry. To elucidate the nature of free radical involvement electron paramagnetic resonance (EPR) studies were carried out. The influence of hyaluronan and its fragments on the concentration of hydroxyl radicals generated by the Fenton system was examined using the spin trapping technique. Moreover, the mucopolysaccharide's ability to react with stable radicals was checked. The quantification assay of 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) showed no concentration changes of the stable radical caused by hyaluronan. Hyaluronan was found to exhibit prooxidative effects in the Fenton assay in a concentration dependent manner. A transition metal chelation was proposed as a mechanism of this behavior. Considering human skin and its constant exposure to UV light and oxygen and an increased pool of iron in irradiated skin the administration of hyaluronan or its fragments in cosmetic formulations or sunscreens could be helpful for the protection of the human skin.
Trommer
Hagen
H
School of Pharmacy, Institute of Pharmaceutics and Biopharmaceutics, Martin-Luther-University, Wolfgang-Langenbeck-Strasse 4, D-06120 Halle (Salle), Germany.
Wartewig
Siegfried
S
Böttcher
Rolf
R
Pöppl
Andreas
A
Hoentsch
Joachim
J
Ozegowski
Jörg H
JH
Neubert
Reinhard H H
RH
D004578:Electron Spin Resonance Spectroscopy; D005296:Ferrous Compounds; D006820:Hyaluronic Acid / Q000096:biosynthesis / Q000737:chemistry*; D015227:Lipid Peroxidation; D008081:Liposomes / Q000737:chemistry / Q000528:radiation effects; D008315:Malondialdehyde / Q000032:analysis; D008563:Membrane Lipids / Q000737:chemistry / Q000528:radiation effects*; D008954:Models, Biological; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000032:analysis; D012867:Skin / Q000737:chemistry / Q000528:radiation effects*; D021241:Spectrometry, Mass, Electrospray Ionization; D018502:Streptococcus equi / Q000378:metabolism; D014466:Ultraviolet Rays
D005296:Ferrous Compounds; D008081:Liposomes; D008563:Membrane Lipids; D017382:Reactive Oxygen Species; C020748:ferrous sulfate; D008315:Malondialdehyde; D006820:Hyaluronic Acid