Determination of limonene oxidation products using SPME and GC-MS.
Limonene is a common component found in consumer goods ranging from beverages to cleaning compounds. Limonene oxidation products, however, have a less desirable flavor and fragrance. Early detection of limonene oxide formation would aid quality control. A method is developed to determine the concentration of limonene oxide in essential oils and beverages using solid-phase microextraction (SPME). A headspace sampling technique is used to reduce or eliminate the presence of less volatile components. Several different SPME fibers are tested, varying in polymer thickness, polymer cross-linking and bonding, and polarity of the polymer. For each fiber tested, the sampling time is optimized for reproducible results. The 7-microm-thick bonded poly(dimethylsiloxane) fiber provides the best results. External standards are used for quantitation.
Marine
Susan Sonchik
SS
Miami University Middletown, 4200 E. University Blvd., Middletown, OH 45005, USA. mariness@muohio.edu
Clemons
Jason
J
D002853:Chromatography, Liquid / Q000379:methods*; D053138:Cyclohexenes; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D000077222:Limonene; D010084:Oxidation-Reduction; D011786:Quality Control; D012015:Reference Standards; D013729:Terpenes / Q000032:analysis*
D053138:Cyclohexenes; D013729:Terpenes; D000077222:Limonene