Competitive interactions of adriamycin and ethidium bromide with DNA as studied by full rank parallel factor analysis of fluorescence three-way array data.
The competitive interactions of adriamycin (AMC) and a fluorescence probe of ethidium bromide (EB) with DNA have been studied by full rank parallel factor analysis (FRA-PARAFAC) of fluorescence excitation-emission three-way data array. The excitation and emission spectra as well as the equilibrium concentrations of co-existing species in different reaction mixtures can be directly obtained by the FRA-PARAFAC treatment. The concordance of the resolved excitation and emission spectra of AMC, EB and EB-DNA with the standard spectra of these species confirmed the reliability of the equilibrium concentrations of these components in the reaction mixtures studied. The results obtained are valuable for providing a deeper insight into the competitive interaction mechanism of AMC and EB with DNA. The conclusion was directly given out that the interaction of AMC with DNA is the intercalating model. The FRA-PARAFAC method as exemplified by the present study provides an useful approach for studying the interaction of clinical drugs with DNA in the presence of disturbance of drug assistants.
Xie
Hong-Ping
HP
State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemometrics and Chemical Engineering, Institute for Chemometrics and Chemical Technology, Hunan University, Changsha 410082, People's Republic of China.
Chu
Xia
X
Jiang
Jian-Hui
JH
Cui
Hui
H
Shen
Guo-Li
GL
Yu
Ru-Qin
RQ
D000465:Algorithms; D000970:Antineoplastic Agents / Q000737:chemistry; D001667:Binding, Competitive; D004247:DNA / Q000378:metabolism*; D004317:Doxorubicin / Q000737:chemistry* / Q000378:metabolism; D004996:Ethidium / Q000737:chemistry* / Q000378:metabolism; D007364:Intercalating Agents / Q000494:pharmacology; D015233:Models, Statistical; D013050:Spectrometry, Fluorescence
D000970:Antineoplastic Agents; D007364:Intercalating Agents; D004317:Doxorubicin; D004247:DNA; D004996:Ethidium