Hepatocyte ploidy in normal young rat.
The aim of the present study was to examine the relation between hepatocyte size and ploidy in Sprague-Dawley rat liver. Therefore, subpopulations of hepatocytes of various sizes were separated from the isolated crude hepatocyte population either mechanically or by using centrifugal elutriation. Hepatocyte size was determined on scanning electron microscopy photographs. Ploidy of hepatocytes was assessed by flow cytometry. The crude hepatocyte population was very heterogeneous in sizes, with diameters ranging from 8 to 39 microm. Hepatocyte ultrastructure was well preserved as demonstrated by transmission electron microscopy. The distribution of hepatocytes within the ploidy classes was the following: 19.6+/-3.6% diploid, 56.2+/-3.2% tetraploid and 3.4+/-0.6% octoploid mononucleated cells. Thus approximately 79% of hepatocytes appeared mononucleated. The binucleated hepatocytes (21%) had two diploid nuclei (18.7+/-2.9%) or two tetraploid nuclei (2.1+/-0.6%). A similar distribution of hepatocytes into ploidy classes was obtained in subpopulations of hepatocytes of various sizes. Our findings suggest that distribution into ploidy classes is not strictly correlated with hepatocyte size. In accordance with previous observations, our results on hepatocyte ploidy from periportal or perivenous origin using digitonin perfusion, is in favour of the existence of ploidy zonation within the rat hepatic lobule.
Gandillet
Arnaud
A
Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, 5, Avenue Molière, 67200, Strasbourg, France.
Alexandre
Eliane
E
Holl
Vincent
V
Royer
Cathy
C
Bischoff
Pierre
P
Cinqualbre
Jacques
J
Wolf
Philippe
P
Jaeck
Daniel
D
Richert
Lysiane
L
D000375:Aging / Q000235:genetics; D000818:Animals; D002458:Cell Fractionation; D048429:Cell Size; D005434:Flow Cytometry; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism*; D008297:Male; D011003:Ploidies; D051381:Rats; D017207:Rats, Sprague-Dawley