Cytochrome P-450 expression and lipid peroxidation in gallbladder cancer.
OBJECTIVE
Carcinoma of the gallbladder is the commonest neoplasm of the biliary tract; however, the etiology of this tumor remains unclear.


METHODS
A case-control study was carried out comprising 50 patients with gallbladder cancer and 50 patients with cholelithiasis. Cytochrome P-450 estimation was carried out in the gallbladder tissue, and lipid peroxide product melonaldehyde (MDA) was measured in gallbladder bile. Statistical analysis was carried out by Student's t-test, and one-way analysis of variance (ANOVA). The Pearson correlation coefficient was calculated, and linear regression analysis was performed.


RESULTS
The mean cytochrome P-450 concentration was significantly higher in patients with carcinoma of the gallbladder than in controls (t = 3.9, P < 0.001; F = 15.2, P = 0.002). Similarly, the mean concentration of melonaldehyde was significantly higher among cancer patients than in patients with gallstones (t = 2.7, P < 0.003; F = 7.37, P = 0.0078). No significant correlation was observed between tissue MDA and cytochrome P-450 levels (Pearson r = 0.1, P = 0.28).


CONCLUSIONS
The results of the present study show that the rise in concentration of MDA is independent of the rise in concentration of cytochrome P-450; however, this is statistically insignificant. This could be due to biliary stasis leading to a rise in biliary concentration of melonaldeyde that is disproportionate to its production.
Pandey
Manoj
M
Department of Surgery, Banaras Hindu University, Varanasi, India. manojpandey@vsnl.com
Sharma
Lal B
LB
Shukla
Vijay K
VK
D000328:Adult; D002277:Carcinoma / Q000201:enzymology* / Q000473:pathology*; D016022:Case-Control Studies; D002769:Cholelithiasis / Q000150:complications; D002779:Cholestasis; D003577:Cytochrome P-450 Enzyme System / Q000494:pharmacology*; D005260:Female; D005706:Gallbladder Neoplasms / Q000201:enzymology* / Q000473:pathology*; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged
D003577:Cytochrome P-450 Enzyme System