Isotype determination of circulating autoantibodies in canine autoimmune subepidermal blistering dermatoses.
The three most common canine autoimmune blistering skin diseases (AISBD), bullous pemphigoid (BP), mucous membrane pemphigoid (MMP) and epidermolysis bullosa acquisita (EBA) have recently been separated based on clinical, histological and immunological grounds. The objectives of this study were to determine the isotype profiles of circulating autoantibodies in these dermatoses. Serum was collected from 5 dogs with BP, 15 with MMP and 11 with EBA. All sera were tested using an indirect immunofluorescence method using salt-split canine gingiva as substrate. Anti-basement membrane IgG autoantibodies were detected in all patients. Among the IgG autoantibodies, IgG1 and IgG4 were encountered most frequently, while IgG2 and IgG3 were uncovered in some dogs. IgE autoantibodies were detected more often than IgA or IgM autoantibodies in any of the three entities. The predominance of IgG1, IgG4 and IgE autoantibody isotypes in dogs with AISBD is very similar to the situation found in humans with the homologous diseases.
Favrot
Claude
C
Université de Montréal, Faculté de Médecine Vétérinaire, Saint-Hyacinthe, Québec, Canada.
Dunston
Stanley M
SM
Department of Clinical Sciences, College of Veterinary Medicine, NC State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA.
Paradis
Manon
M
Université de Montréal, Faculté de Médecine Vétérinaire, Saint-Hyacinthe, Québec, Canada.
Olivry
Thierry
T
Department of Clinical Sciences, College of Veterinary Medicine, NC State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA.
D000818:Animals; D001323:Autoantibodies / Q000097:blood*; D001327:Autoimmune Diseases / Q000276:immunology / Q000662:veterinary; D004283:Dog Diseases / Q000097:blood / Q000276:immunology*; D004285:Dogs; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D007132:Immunoglobulin Isotypes / Q000097:blood*; D008297:Male; D012872:Skin Diseases, Vesiculobullous / Q000276:immunology / Q000662:veterinary*
D001323:Autoantibodies; D007132:Immunoglobulin Isotypes