title: Potassium channel expression level is dependent on the proliferation state in the GH3 pituitary cell line.
abstract: Previously, we showed that the peak density of the transient outward K(+) current (I(to)) expressed in GH3 cells was different in the S phase than in other phases of the cell cycle. Using cell synchronization, we show here that I(to) drops precisely at the quiescent (G(0) phase)/proliferating transition. This change is not due to a modification in the voltage dependence of I(to), but rather to a modification in its inactivation kinetics. Molecular determination of K(+) channel subunits showed that I(to) required the expression of Kv1.4, Kv4.1, and Kv4.3. We found that the increase in I(to) density during the quiescent state was accompanied by an increase in Kv1.4 protein expression, whereas Kv4.3 expression remained unchanged. We further demonstrate that the link between I(to) expression and cell proliferation is not mediated by variations in cell excitability. These results provide new evidence for the cell cycle dependence of I(to) expression, which could be relevant in understanding the mechanisms leading to pituitary adenomas.
authors.affiliation: Laboratoire de Physiologie et Physiopathologie de la Signalisation Cellulaire, CNRS UMR 5543, Université de Bordeaux 2, 33076 Cedex Bordeaux, France.
authors.forename: Antonny
authors.initials: A
authors.lastname: Czarnecki
authors.forename: Luce
authors.initials: L
authors.lastname: Dufy-Barbe
authors.forename: Sylvie
authors.initials: S
authors.lastname: Huet
authors.forename: Marie-Francoise
authors.initials: MF
authors.lastname: Odessa
authors.forename: Laurence
authors.initials: L
authors.lastname: Bresson-Bepoldin
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D004594:Electrophysiology; D016193:G1 Phase / Q000502:physiology; D051665:Kv1.4 Potassium Channel; D010902:Pituitary Gland / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology; D015221:Potassium Channels / Q000378:metabolism* / Q000502:physiology; D024642:Potassium Channels, Voltage-Gated; D016192:Resting Phase, Cell Cycle / Q000502:physiology; D051672:Shal Potassium Channels; D013997:Time Factors
chemical_list: D051665:Kv1.4 Potassium Channel; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D051672:Shal Potassium Channels