RpoD promoters in Campylobacter jejuni exhibit a strong periodic signal instead of a -35 box.
We have used a hidden Markov model (HMM) to identify the consensus sequence of the RpoD promoters in the genome of Campylobacter jejuni. The identified promoter consensus sequence is unusual compared to other bacteria, in that the region upstream of the TATA-box does not contain a conserved -35 region, but shows a very strong periodic variation in the AT-content and semi-conserved T-stretches, with a period of 10-11 nucleotides. The TATA-box is in some, but not all cases, preceded by a TGx, similar to an extended -10 promoter. We predicted a total of 764 presumed RpoD promoters in the C.jejuni genome, of which 654 were located upstream of annotated genes. A similar promoter was identified in Helicobacter pylori, a close phylogenetic relative of Campylobacter, but not in Escherichia coli, Vibrio cholerae, or six other Proteobacterial genomes, or in Staphylococcus aureus. We used upstream regions of high confidence genes as training data (n=529, for the C.jejuni genome). We found it necessary to limit the training set to genes that are preceded by an intergenic region of >100bp or by a gene oriented in the opposite direction to be able to identify a conserved sequence motif, and ended up with a training set of 175 genes. This leads to the conclusion that the remaining genes (354) are more rarely preceded by a (RpoD) promoter, and consequently that operon structure may be more widespread in C.jejuni than has been assumed by others. Structural predictions of the regions upstream of the TATA-box indicates a region of highly curved DNA, and we assume that this facilitates the wrapping of the DNA around the RNA polymerase holoenzyme, and offsets the absence of a conserved -35 binding motif.
Petersen
Lise
L
Center for Biological Sequence Analysis, Technical University of Denmark, DK-2800 Lyngby, Denmark. lpe@vetinst.dk
Larsen
Thomas S
TS
Ussery
David W
DW
On
Stephen L W
SL
Krogh
Anders
A
D001426:Bacterial Proteins / Q000235:genetics*; D001665:Binding Sites; D016123:Campylobacter jejuni / Q000235:genetics*; D016384:Consensus Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004926:Escherichia coli; D005786:Gene Expression Regulation; D008957:Models, Genetic; D009876:Operon; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012808:Sigma Factor / Q000235:genetics*; D016385:TATA Box; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012808:Sigma Factor; D014157:Transcription Factors; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases