title: Induction of apoptosis in an insect cell line, IPLB-Ld652Y, infected with nucleopolyhedroviruses.
abstract: Ld652Y cells derived from the gypsy moth, Lymantria dispar, were infected with seven different nucleopolyhedroviruses (NPVs) including those from Autographa californica, Bombyx mori (BmNPV), Hyphantria cunea (HycuNPV), Spodoptera exigua (SeMNPV), L. dispar, Orgyia pseudotsugata (OpMNPV) and Spodoptera litura (SpltMNPV). The results showed that Ld652Y cells infected with BmNPV, HycuNPV, SeMNPV, OpMNPV and SpltMNPV underwent apoptosis, displaying apoptotic bodies, characteristic DNA fragmentation and increased caspase-3-like protease activity; HycuNPV induced the most severe apoptosis. In HycuNPV-infected Ld652Y cells, a considerable amount of viral DNA was synthesized although there was no detectable yield of budded virions and polyhedrin. Northern blot and immunoblot analyses revealed that HycuNPV inhibitor of apoptosis 3 (IAP3), which has been shown to function in Sf9 cells, was expressed in HycuNPV-infected Ld652Y cells at a level higher than or comparable with that in HycuNPV-infected SpIm cells, which produced a high titre of progeny virions without any apoptotic response. These results imply that the relative ease of apoptosis induction in NPV-infected Ld652Y cells is largely dependent on inherent cellular properties rather than functions of the respective NPVs, and indicate that the defect in progeny virion production is not merely due to the virus-induced apoptosis in HycuNPV-infected Ld652Y cells.
authors.affiliation: Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ishikawa
authors.affiliation: Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Motoko
authors.initials: M
authors.lastname: Ikeda
authors.affiliation: Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yanagimoto
authors.affiliation: Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Cristiano A Felipe
authors.initials: CAF
authors.lastname: Alves
authors.affiliation: Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Katou
authors.affiliation: Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Laviña-Caoili
authors.affiliation: Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Michihiro
authors.initials: M
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D017209:Apoptosis; D015152:Blotting, Northern; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D053938:DNA Fragmentation; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis; D015151:Immunoblotting; D002479:Inclusion Bodies; D051034:Inhibitor of Apoptosis Proteins; D007313:Insecta; D017924:Nucleopolyhedroviruses / Q000378:metabolism / Q000502:physiology*; D000077183:Occlusion Body Matrix Proteins; D012333:RNA, Messenger / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis; D015678:Viral Structural Proteins / Q000032:analysis / Q000096:biosynthesis; D014771:Virion / Q000302:isolation & purification; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D051034:Inhibitor of Apoptosis Proteins; D000077183:Occlusion Body Matrix Proteins; D012333:RNA, Messenger; D014764:Viral Proteins; D015678:Viral Structural Proteins; C086430:inhibitor of apoptosis, Nucleopolyhedrovirus; C009829:polyhedrin protein, Nucleopolyhedrovirus; D020169:Caspases