[Effects of mitogen-activated protein kinase(MAPK) in cell signal transduction of chronic myelogenous leukemia cell line K562].
OBJECTIVE
Ras-MAPK signal transduction has been thought to play an important role in the carcinogenesis of chronic myelogenous leukemia. In this study, the authors investigated the effects and mechanism of mitogen-activated protein kinase (MAPK) in cell signal transduction of chronic myelogenous leukemia(CML).


METHODS
After MAPK antisense oligodeoxynucleotide (ASO) was introduced into K562 cell line by liposomal transfection, the effects of ASO on K562 cell were evaluated by cell proliferation, DNA synthesis, MAPK protein content and MAPK activity.


RESULTS
The cell proliferation, DNA synthesis, MAPK protein content and MAPK activity were significantly inhibited by MAPK ASO, the inhibitory rates were 51.8%, 57.1%, 45.3%, and 61.6%, respectively,with significant difference in comparison to the control (P<0.05).


CONCLUSIONS
MAPK plays an important role in the signal transduction of CML and MAPK may become a new target in the treatment of CML.
Shang
Zhen-Chuan
ZC
Department of Hematology, Xijing Hospital, Xi'an, Shaanxi, 710032, PR China. shangzc2002@yahoo.com.cn
Sun
Bing-Zhong
BZ
Chen
Zhi-Nan
ZN
Wang
Wei
W
Feng
Qi
Q
Wang
Sha
S
Zhang
Tao
T
D004273:DNA, Neoplasm / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D020319:Oligodeoxyribonucleotides, Antisense; D020928:Mitogen-Activated Protein Kinases