title: Peptostreptococcus micros in primary endodontic infections as detected by 16S rDNA-based polymerase chain reaction.
abstract: A 16S rDNA-based polymerase chain reaction (PCR) method was used to detect Peptostreptococcus micros in primary root canal infections. Samples were collected from 50 teeth having carious lesions, necrotic pulps, and different forms of periradicular diseases. DNA extracted from the samples was amplified using the PCR assay, which yielded a specific fragment of P. micros 16S rDNA. P. micros was detected in 6 of 22 root canals associated with asymptomatic chronic periradicular lesions (27.3%), 2 of 8 teeth with acute apical periodontitis (25%), and 6 of 20 cases of acute periradicular abscess (30%). In general, P. micros was found in 14 of 50 cases (28%). There was no correlation between the presence of P. micros and the occurrence of symptoms. Findings suggested that P. micros can be involved in the pathogenesis of different forms of periradicular lesions.
authors.affiliation: Oral Microbiology Laboratory, Institute of Microbiology, Federal University of Rio de Janeiro, Brazil.
authors.forename: José F
authors.initials: JF
authors.lastname: Siqueira
authors.forename: Isabela N
authors.initials: IN
authors.lastname: Rôças
authors.forename: Arnaldo F B
authors.initials: AF
authors.lastname: Andrade
authors.forename: Milton
authors.initials: M
authors.lastname: de Uzeda
mesh_terms: D000293:Adolescent; D000328:Adult; D015373:Bacterial Typing Techniques; D016009:Chi-Square Distribution; D004269:DNA, Bacterial / Q000032:analysis; D003790:Dental Pulp Necrosis / Q000382:microbiology*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008875:Middle Aged; D016017:Odds Ratio; D010462:Peptostreptococcus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D010485:Periapical Periodontitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S