DNA extraction using bacterial magnetic particles modified with hyperbranched polyamidoamine dendrimer.
A cascading hyperbranched polyamidoamine dendrimer was synthesized on the surface of bacterial magnetite from Magnetospirillum magneticum AMB-1 to allow enhanced extraction of DNA from fluid suspensions. Characterization of the synthesis revealed linear doubling of the surface amine charge from generations one through five starting with an amino silane initiator. Furthermore, transmission electron microscopy revealed clear dispersion of the single domain magnetite in aqueous solution. The dendrimer modified magnetic particles have been used to carry out magnetic separation of DNA. Binding and release efficiencies increased with the number of generations and those of bacterial magnetite modified with six generation dendrimer were 7 and 11 times respectively as many as those of bacterial magnetite modified with only amino silane.
Yoza
Brandon
B
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16, Koganei, Japan.
Arakaki
Atsushi
A
Matsunaga
Tadashi
T
D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D004247:DNA / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification* / Q000648:ultrastructure; D050091:Dendrimers; D052203:Ferrosoferric Oxide; D006801:Humans; D007501:Iron / Q000737:chemistry*; D046911:Macromolecular Substances; D008280:Magnetics; D008832:Microchemistry / Q000379:methods; D008863:Microspheres; D010087:Oxides / Q000737:chemistry*; D010316:Particle Size; D011073:Polyamines / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D012244:Rhodospirillaceae / Q000737:chemistry*; D013535:Suspensions
D020099:Coated Materials, Biocompatible; D050091:Dendrimers; D046911:Macromolecular Substances; D010087:Oxides; C104700:PAMAM Starburst; D011073:Polyamines; D011108:Polymers; D013535:Suspensions; D004247:DNA; D007501:Iron; D052203:Ferrosoferric Oxide