The expression of mRNA for calcitonin receptor-like receptor/receptor-activity modifying proteins in rat peritoneal mast cells.
Adrenomedullin is a hypotensive peptide secreted from various cells. Recently, we found that adrenomedullin, but not calcitonin gene-related peptide (CGRP), stimulates histamine release from rat peritoneal mast cells. In the present studies, we investigated the expression of mRNA for calcitonin-receptor-like receptor (CRLR) and receptor-activity modifying proteins (RAMPs), the components of proposed adrenomedullin receptors, in rat peritoneal mast cells by reverse transcription-polymerase chain reaction (RT-PCR). Results revealed that mRNA for CRLR, RAMP2 and RAMP3 was expressed in rat peritoneal mast cells, whereas mRNA for RAMP1 was not. These data suggest that adrenomedullin might stimulate histamine release via its proposed receptor (CRLR/RAMP2 or 3), rather than via the CGRP receptor (CRLR/RAMP1).
Matsushima
Miyoko
M
Department of Medical Technology, Nagoya University School of Health Sciences, 1-1-20 Daikominami, Higashi-ku, Japan.
Kitaichi
Kiyoyuki
K
Tatsumi
Yasuaki
Y
Hasegawa
Takaaki
T
Takagi
Kenzo
K
D000818:Animals; D058286:Calcitonin Receptor-Like Protein; D015870:Gene Expression; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics*; D010529:Peritoneal Cavity / Q000166:cytology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D058262:Receptor Activity-Modifying Protein 1; D058263:Receptor Activity-Modifying Protein 2; D058264:Receptor Activity-Modifying Protein 3; D058261:Receptor Activity-Modifying Proteins; D018003:Receptors, Calcitonin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D058286:Calcitonin Receptor-Like Protein; C545580:Calcrl protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C545508:Ramp1 protein, rat; C545509:Ramp2 protein, rat; C545510:Ramp3 protein, rat; D058262:Receptor Activity-Modifying Protein 1; D058263:Receptor Activity-Modifying Protein 2; D058264:Receptor Activity-Modifying Protein 3; D058261:Receptor Activity-Modifying Proteins; D018003:Receptors, Calcitonin