title: Effect of long-term high-fat feeding on the expression of pancreatic lipases and adipose tissue uncoupling proteins in mice.
abstract: BACKGROUND
  A diet containing a high amount of fat has been shown, in short-term studies, to increase the expression of pancreatic lipase and colipase.
  
  
  OBJECTIVE
  To investigate the effects of long-term high-fat-feeding (113 days) on the mRNA expression of pancreatic lipase, colipase, pancreatic lipase-related proteins (1 and 2), and uncoupling proteins during the development of obesity and glucose intolerance.
  
  
  METHODS
  Mice were fed either a high-fat or standard diet and killed after 3, 13, 57, and 113 days. Brown and white adipose tissue and pancreas were collected for mRNA extraction [corrected].
  
  
  RESULTS
  The high-fat-fed mice became obese and glucose-intolerant by 113 days. The high-fat diet increased lipase (p < 0.05) expression initially. At the end of the experiment, the lipase levels had decreased to the level of the control. Colipase levels did not change during the first 57 days of high-fat feeding but decreased below control levels by 113 days (p < 0.05). The high-fat diet increased brown adipose tissue uncoupling protein 1 (UCP1)(p < 0.005) expression but not the expression of uncoupling protein 2.
  
  
  CONCLUSIONS
  Long-term high-fat feeding, leading to glucose intolerance, occurs with a simultaneous decrease in the mRNA expression of pancreatic lipase and colipase and an increase in UCP1 expression.
authors.affiliation: Department of Cell and Molecular Biology, Section for Molecular Signaling, University of Lund, Lund, Sweden. catarina.rippe@medkem.lu.se
authors.forename: Catarina
authors.initials: C
authors.lastname: Rippe
authors.forename: Karin
authors.initials: K
authors.lastname: Berger
authors.forename: Jie
authors.initials: J
authors.lastname: Mei
authors.forename: Mark E
authors.initials: ME
authors.lastname: Lowe
authors.forename: Charlotte
authors.initials: C
authors.lastname: Erlanson-Albertsson
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003089:Colipases / Q000235:genetics / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage*; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007473:Ion Channels; D008049:Lipase / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D026901:Membrane Transport Proteins; D051379:Mice; D008815:Mice, Inbred Strains; D024101:Mitochondrial Proteins; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2
chemical_list: D002352:Carrier Proteins; D003089:Colipases; D004041:Dietary Fats; D007473:Ion Channels; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D011506:Proteins; D012333:RNA, Messenger; C000604372:Ucp1 protein, mouse; C000604390:Ucp2 protein, mouse; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2; D008049:Lipase; C075988:pancreatic lipase related protein 2; C075987:pancreatic lipase-related protein 1