[Assessment of the estrogenicity of endosulfan and other chemicals with the in vitro proliferation of human breast cancer cell]
OBJECTIVE: To develop a rapid screening method for xenoestrogens and to screen the estrogenicity of some environmental chemicals. METHODS: The E-SCREEN test was developed based on proliferation of human breast cancer cells (MCF-7), and the estrogenicity of diethylstilbestrol, 4-hydrotamoxifen and endosulfan was assessed. RESULTS: The E-SCREEN detected estradiol at very low concentration as 1x10(-13) mol/L. It was found that diethylstilbestrol was a full agonist of estrogen receptor, endosulfan was a partial agonist, while 4 hydrotamoxifen lacked estrogenic effects at this assay. CONCLUSION: The E-SCREEN test is sensitive, rapid, easy to perform and, therefore, suitable for large scale screening for estrogenicity of environmental chemicals.
Chang
Yan
Y
School of Public Health, College of Medical Science, Zhejiang University, Hangzhou 310006 China.
Zhu
Xin-Qiang
XQ
Zhu
Hui-Juan
HJ
Zheng
Yi-Fan
YF
Huang
Xing-Shu
XS