Lack of anti-D in women at birth following antepartum immune globulin prophylaxis.
Antepartum prophylaxis using Rh immune globulin suppresses maternal immunization to transplacental transfer of Rh-positive fetal cells, and, theoretically, to be effective, anti-D should be detectable until birth. We used a sensitive gel technique to quantitatively detect the serum concentration of anti-D at birth in 150 women who had received 300 microg of Rh immune globulin at 28 weeks gestation. Our method, which was sensitive enough to detect 20-25 microg, the recommended residual amount at birth, was positive in only 21% total, and in only 13% of women at term. Fifty-seven percent of women with premature births had above the recommended level of anti-D.
Rudensky
Bernard
B
Laboratory of Immunohaematology, Shaare Zedek Medical Center, Faculty of Health Sciences, Ben Gurion University of the Negev, Be'er Sheva, Jerusalem 91031, Israel. rudensky@szm.org.il
Mazaki
Eyal
E
Na'amad
Mira
M
Samueloff
Arnon
A
D005260:Female; D005865:Gestational Age; D006801:Humans; D007518:Isoantibodies / Q000097:blood*; D007752:Obstetric Labor, Premature; D036801:Parturition; D011247:Pregnancy; D018029:Rho(D) Immune Globulin / Q000008:administration & dosage* / Q000627:therapeutic use; D012680:Sensitivity and Specificity; D013997:Time Factors
D007518:Isoantibodies; C033242:RH-antibodies; D018029:Rho(D) Immune Globulin