Intraspecies variability of Desulfovibrio desulfuricans strains determined by the genetic profiles.
Fifteen (soil and intestinal) strains of Desulfovibrio desulfuricans species were typed by PCR method with the use of primers specific for repetitive extragenic palindromic (REP) and enterobacterial repetitive intergenic consensus (ERIC) sequences. As a result, characteristic DNA fingerprints for the strains were obtained. Moreover, the genetic profiles were found to be useful for typing and distinguishing the strains of D. desulfuricans. According to cluster analysis, PCR with primers complementary to the sequences REP appeared to be slightly more discriminatory than PCR with ERIC primers for the investigated strains. Distinct fingerprint patterns of two isolates derived from the same patient pointed to the different origin of both strains.
Dzierzewicz
Zofia
Z
Department of Molecular Biology, Medical University of Silesia, Narcyzów 1, 41-200, Sosnowiec, Poland. zofiadz@slam.katowice.pl
Szczerba
Joanna
J
Weglarz
Ludmila
L
Swiatkowska
Longina
L
Jasinska
Dorota
D
Wilczok
Tadeusz
T
D015373:Bacterial Typing Techniques; D002432:Cecum / Q000382:microbiology; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D003901:Desulfovibrio / Q000145:classification* / Q000235:genetics; D005243:Feces / Q000382:microbiology; D014644:Genetic Variation; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012988:Soil Microbiology; D013045:Species Specificity
D017931:DNA Primers; D004269:DNA, Bacterial