[Antiviral effect of alpha-interferon and cytokine mRNA level in cell cultures infected with a cytopathogenic variant of the hepatitis C virus].
An experimental model of the viral C-hepatitis (VCH) infection was worked out in vitro and it was found suitable to study the influence of interferon (IFN) preparations produced on the infection caused by an HCV cytopathogenic variations, i.e. the SW-13 human adrenocarcinoma cellular culture sensitive to the anti-VCH action of alpha-IFN and the MT-4 human lymphoblastoid cellular culture non-sensitive to the anti-VCH action of alpha-IFN. The above cellular models were employed to study, by using the methods of reverse transcription and polymerize chain reaction (RT-PCR), the influence produced by alpha-IFN on the VCH infectious activity as well as to study the changes in the activity of the below cytokine mRNAs: alpha-IFN, gamma-IFN, IL-1 beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-18 and TNF-alpha. A double treatment of the SW-13 alpha-IFN cellular cultures 24 and 48 hours after the infection was found to essentially suppress (by 4 Ig) reproduction of the VCH cytopathogenic variant. It was detected that the VCH reproduction is mediated by the regulation of a number of cytokine genes. The study results can be a basis for a more effective use of the alpha-IFN preparations in the therapy of VCH-infections.
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D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D016174:Hepacivirus / Q000187:drug effects* / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000998:Antiviral Agents; D016207:Cytokines; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D012333:RNA, Messenger; D012367:RNA, Viral; D011994:Recombinant Proteins