Pheromonal stimulation of spawning release of gametes by gonadotropin releasing hormone in the chiton, Mopalia sp.
The chiton Mopalia sp., a mollusc, was exposed to various dilutions of gonadotropin releasing hormone (GnRH) in sea water to determine whether this peptide is capable of acting as a pheromone that could stimulate release of ripe gametes (spawning). Two of the peptides, lamprey GnRH-1 and tunicate GnRH-2, had this action at a higher concentration (1.0 mg/L) but dilutions to 50 microg/L no longer were effective. Three other GnRHs: lamprey GnRH-3, tunicate GnRH-1, and a modified chicken GnRH-2, had no such action under the same test conditions. Since the spawning response could be produced by some GnRHs and not by others, it would appear that some kind of molecular recognition is involved, possibly by specific binding to a receptor. In earlier preliminary experiments tunicate GnRH-2 rapidly stimulated gamete release in a hemichordate, Saccoglossus. Thus it is suggested that GnRHs, in at least some invertebrates, may function as pheromones, serving to stimulate simultaneous spawning of individuals in a population of animals, and in this way assure more successful fertilization in species that must release their gametes into the water in which they live.
Gorbman
Aubrey
A
Department of Zoology, University of Washington, Seattle, WA 98195, USA. agorbman@aol.com
Whiteley
Arthur
A
Kavanaugh
Scott
S
D000818:Animals; D002645:Chickens / Q000378:metabolism; D005260:Female; D005854:Germ Cells / Q000187:drug effects*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D007536:Isomerism; D007798:Lampreys / Q000378:metabolism; D008297:Male; D008974:Mollusca / Q000378:metabolism*; D012621:Seasons; D012724:Sex Attractants / Q000494:pharmacology*; D012726:Sexual Behavior, Animal / Q000187:drug effects*; D014561:Urochordata / Q000378:metabolism
D012724:Sex Attractants; D007987:Gonadotropin-Releasing Hormone