title: Laboratory acquired infection with recombinant vaccinia virus containing an immunomodulating construct.
abstract: Handling of Vaccinia virus represents a risk for laboratory-acquired infections, especially in individuals without completed vaccination. We report the case of a Vaccinia infection in a previously vaccinated researcher working with various genetically modified strains. We could confirm the infection by electron microscopy, positive cell culture, virus-specific PCR, sequence analysis, and viral neutralization test. The isolated virus carried a functionally inactivated cytohesin-1 gene of human origin, which had been shown to impair leukocyte adhesion by interacting with the LFA/ICAM-1 axis. The immunomodulating nature of the inserted construct might thus have added to the infectivity of the virus. We emphasize on the necessity of Vaccinia vaccination in laboratory staff working in the field.
authors.affiliation: Department of Dermatology and Allergy, Biederstein, Technical University Munich, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Mempel
authors.forename: Gisela
authors.initials: G
authors.lastname: Isa
authors.forename: Norbert
authors.initials: N
authors.lastname: Klugbauer
authors.forename: Hermann
authors.initials: H
authors.lastname: Meyer
authors.forename: Gregor
authors.initials: G
authors.lastname: Wildi
authors.forename: Johannes
authors.initials: J
authors.lastname: Ring
authors.forename: Franz
authors.initials: F
authors.lastname: Hofmann
authors.forename: Heidelore
authors.initials: H
authors.lastname: Hofmann
mesh_terms: D000276:Adjuvants, Immunologic / Q000235:genetics*; D000328:Adult; D001483:Base Sequence / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000235:genetics; D020868:Gene Silencing; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D007757:Laboratory Infection; D008297:Male; D008969:Molecular Sequence Data; D030781:Organisms, Genetically Modified / Q000235:genetics*; D011995:Recombination, Genetic; D014611:Vaccination; D014615:Vaccinia / Q000175:diagnosis / Q000473:pathology / Q000517:prevention & control / Q000821:virology*; D014616:Vaccinia virus / Q000235:genetics*; D014765:Viral Vaccines / Q000627:therapeutic use
chemical_list: D000276:Adjuvants, Immunologic; D015815:Cell Adhesion Molecules; D020662:Guanine Nucleotide Exchange Factors; D014765:Viral Vaccines; C100723:cytohesin-1