Differential screening of cDNA libraries for analysis of gene expression during tumor progression.
The ability to examine gene expression differences during cancer progression is crucial to our understanding of the biological events that lead to uncontrolled growth of malignant cells. Differential expression screening of complementary DNA libraries is described in detail here as a valuable method for monitoring gene expression changes in a prostate cancer xenograft model. Using Southern blot analysis of complementary DNA clones and Northern blot analysis to confirm differential expression, several androgen-regulated transcripts have been identified that may be important in prostate cancer progression.
Gregory
Christopher W
CW
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
D004273:DNA, Neoplasm / Q000032:analysis; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis
D004273:DNA, Neoplasm; D012333:RNA, Messenger