Lyme disease spirochete, Borrelia burgdorferi endemic at epicenter in Rondeau Provincial Park, Ontario.
The Lyme disease spirochete, Borrelia burgdorferi Johnson, Schmidt, Hyde, Steigerwalt, and Brenner was discovered in blacklegged ticks, Ixodes scapularis Say at Rondeau Provincial Park, Ontario, Canada During this 2-yr study, spirochetes were found in B. burgdorferi-positive I. scapularis larvae attached to B. burgdorferi-infected white-footed mice, Peromyscus leucopus Rafinesque. Isolates of B. burgdorferi were cultured from blacklegged tick adults, and confirmed positive with polymerase chain reaction by targeting OspA and rrf (5S)-rrl (23S) genes. These findings show an endemic area for B. burgdorferi within an established population of L. scapularis at Rondeau Provincial Park.
Morshed
Muhammad G
MG
Laboratory Services, British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada V5Z 4R4. mmorshed@interchange.ubc.ca
Scott
John D
JD
Fernando
Keerthi
K
Mann
Robert B
RB
Durden
Lance A
LA
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001428:Bacterial Vaccines; D001483:Base Sequence; D025065:Borrelia burgdorferi / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D017931:DNA Primers; D007303:Insect Vectors; D018884:Ixodes; D008074:Lipoproteins; D008193:Lyme Disease / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D051379:Mice; D009864:Ontario / Q000453:epidemiology; D016133:Polymerase Chain Reaction
D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D017931:DNA Primers; D008074:Lipoproteins; C072045:OspA protein