Three-dimensional reconstruction studies and morphometric analysis of rudimental tooth primordia in the upper incisor region of the sheep (Ovis aries, Ruminantia).
The functional dentition of the domestic sheep lacks all upper incisors and the upper canines. Nevertheless, occurrence of a dental lamina and rudimental tooth primordia had been described in the upper incisor region of the sheep. The aim of this study was to describe temporo-spatial pattern of origin and regression of these rudimental tooth primordia by light microscopy, computer-aided three-dimensional reconstruction and morphometry of the dental epithelium. Transient existence of a dental lamina in the upper incisor region of the sheep and three epithelial thickenings on its deep mesenchymal margin has been observed at day of ontogeny (DO) 48-53. They could not been identified as full-value tooth primordia, because they did not induce differentiation of tooth mesenchyme, but they could represent last remnants of functional upper incisors in early ancestors of ruminants. Additionally, a large rudimental upper canine primordium near the sutura maxilloincisiva occurred at DO43, reached early cap stage at DO52 and started to regress at DO53. Thus, our findings showed a discrepancy between the embryonic and adult dental pattern in the sheep. Similar molecular mechanisms as described for diastemal tooth rudiments in rodents could be involved during regression of rudimental tooth primordia in the upper incisor region of the sheep.
Witter
Kirsti
K
Laboratory of Genetics and Embryology, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Veveri; 97, 602 00 2, Brno, Czech Republic. kwitter@iach.cz <kwitter@iach.cz>
Matulová
Petra
P
Mísek
Ivan
I
D000818:Animals; D004848:Epithelium / Q000033:anatomy & histology / Q000196:embryology; D007091:Image Processing, Computer-Assisted; D007180:Incisor / Q000033:anatomy & histology / Q000196:embryology*; D009805:Odontogenesis; D012756:Sheep; D034561:Sheep, Domestic / Q000033:anatomy & histology / Q000196:embryology*; D014083:Tooth Germ / Q000033:anatomy & histology / Q000196:embryology*