title: Essential role of magnesium ion in water for colonization of Helicobacter pylori in 2-week-old miniature pigs.
abstract: This study was designed to determine whether magnesium ion in water would influence the colonization of Helicobacter pylori in 2-week-old miniature pigs. Groups A (2 pigs) and B (1 pig) were both fed a milk diet dissolved in drinking water, Group C (2 pigs) was fed a milk diet dissolved in deionized distilled water (DDW), and Group D (1 pig) was fed a milk diet dissolved in DDW supplemented with MgCl2. Groups B, C, and D were all challenged with H. pylori, and Group A was not. Necropsy was performed on the pigs on postinfection Day 5, and biopsy specimens were taken from 16 sites of the stomach. H. pylori were recovered from 11 of 16 sites in Group B, 1 of 32 sites in Group C, and 13 of 16 sites in Group D. On the other hand, the degree of lymphocyte infiltration increased in the order of Group A < Group B < Group C < Group D. These observations suggest that magnesium ion in drinking water is essential for the colonization of H. pylori in the pig stomach. Possible mechanisms for the lymphocyte infiltration are discussed.
authors.affiliation: Biological Research Laboratories, Sankyo Co., Ltd., Shinagawa-ku, Tokyo, Japan. tekoga@shina.sankyo.co.jp
authors.forename: Tetsufumi
authors.initials: T
authors.lastname: Koga
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Sato
authors.forename: Yukio
authors.initials: Y
authors.lastname: Shimada
authors.forename: Keigo
authors.initials: K
authors.lastname: Takahashi
authors.forename: Isamu
authors.initials: I
authors.lastname: Kikuchi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Okazaki
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Katsuta
authors.forename: Masayuki
authors.initials: M
authors.lastname: Iwata
mesh_terms: D000818:Animals; D002299:Cardia / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D015169:Colony Count, Microbial / Q000379:methods; D005748:Gastric Fundus / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D005753:Gastric Mucosa / Q000187:drug effects / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000187:drug effects / Q000254:growth & development*; D008214:Lymphocytes / Q000473:pathology; D008274:Magnesium / Q000494:pharmacology*; D008297:Male; D011706:Pyloric Antrum / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D013552:Swine; D013556:Swine, Miniature / Q000382:microbiology*; D014881:Water Supply / Q000032:analysis*
chemical_list: D008274:Magnesium