Identification of Histoplasma capsulatum from culture extracts by real-time PCR.
We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.
Martagon-Villamil
Jose
J
Division of Infectious Diseases. Section of Clinical Microbiology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
Shrestha
Nabin
N
Sholtis
Mary
M
Isada
Carlos M
CM
Hall
Gerri S
GS
Bryne
Terry
T
Lodge
Barbara A
BA
Reller
L Barth
LB
Procop
Gary W
GW
D004271:DNA, Fungal / Q000032:analysis; D006658:Histoplasma / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008828:Microbiological Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*
D004271:DNA, Fungal