[Generation of dendritic cells from healthy human peripheral blood].
OBJECTIVE
To get high quality and sufficient numbers of mature dendritic cells (DC) from healthy human peripheral blood.


METHODS
Cultured plastic-adherent monocytes were isolated from healthy human peripheral blood by use of granulocyte-monocyte clony-stimulating factor and Interleukin-4 for 7 days without fed with fresh medium and cytokine.


RESULTS
A large number of DCs with high purity were generated. These DCs expressed HLA-I, II molecules, Costimulating molecules and adherent molecules highly, showing the characteristics of mature DC. These DCs could stimulate proliferation of allogenic T lymphocytes and had active endocytosis ability which peaked at the third day in culture but decreased afterwards.


CONCLUSIONS
These results provide evidence that CD monocytes isolated from peripheral blood monocytes exhibit dendritic cells characteristics and may facilitate further studies of DC and its clinical application.
Guo
Jianwei
J
Department of Imunology, West China School of Precilical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Cai
Meiying
M
D002469:Cell Separation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D015234:HLA-A Antigens / Q000378:metabolism; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D009000:Monocytes / Q000166:cytology* / Q000276:immunology
D015234:HLA-A Antigens; D018950:Lipopolysaccharide Receptors