Site-specific cleavage of human telomerase RNA using PNA-neocuproine.Zn(II) derivatives.
Here we report the synthesis of a novel PNA based neocuproine.Zn RNA cleaving agent; we demonstrate that such agents sequence specifically cleave a synthetic RNA target and in particular the RNA component of human telomerase.
Whitney
Andrew
A
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK CB2 1EW.
Gavory
Gérald
G
Balasubramanian
Shankar
S
D001665:Binding Sites; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000494:pharmacology; D010618:Phenanthrolines / Q000737:chemistry* / Q000494:pharmacology; D012313:RNA; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000737:chemistry* / Q000378:metabolism; D019098:Telomerase / Q000737:chemistry* / Q000378:metabolism; D015032:Zinc / Q000737:chemistry*
D020135:Peptide Nucleic Acids; D010618:Phenanthrolines; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; C422952:telomerase RNA; D012313:RNA; C002701:neocuproine; D019098:Telomerase; D015032:Zinc