Double immunolabeling by the RBM and the PLAP markers for identifying intratubular (in situ) germ cell neoplasia of the testis.
Identification of intratubular germ cell neoplasia (carcinoma in situ, CIS) of the testis is a diagnostic challenge, and markers are sorely needed to assist in accurately identifying the lesion. RNA-binding motif (RBM) protein, encoded by the Y chromosome, is expressed exclusively and consistently in differentiated male germ cells, while it is absent in neoplastic germ cells. Another immunohistochemical marker, placental alkaline phosphatase (PLAP), is commonly used for the detection of undifferentiated germ cells. The current study demonstrates that simultaneous use of the immunohistochemical markers, RBM and PLAP, by double immunolabeling enhances the accuracy of diagnosing CIS, a preinvasive testicular neoplasm.
Schreiber
Letizia
L
Institute of Pathology, Lis Maternity Hospital, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
Lifschitz-Mercer
Beatriz
B
Paz
Gedalia
G
Yavetz
Haim
H
Elliott
David J
DJ
Kula
Krzysztof
K
Slowikowska-Hilczer
Jolanta
J
Maymon
Batia Bar-Shira
BB
D000469:Alkaline Phosphatase; D014408:Biomarkers, Tumor / Q000032:analysis; D002278:Carcinoma in Situ / Q000737:chemistry* / Q000473:pathology; D002675:Child, Preschool; D019084:Fluorescent Antibody Technique, Indirect; D058851:GPI-Linked Proteins; D018237:Germinoma / Q000737:chemistry* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D016601:RNA-Binding Proteins / Q000032:analysis*; D013736:Testicular Neoplasms / Q000737:chemistry* / Q000473:pathology
D014408:Biomarkers, Tumor; D058851:GPI-Linked Proteins; D007527:Isoenzymes; D016601:RNA-Binding Proteins; D000469:Alkaline Phosphatase; C019839:alkaline phosphatase, placental