Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli.
The gene encoding a putative mature antitumor-analgesic peptide (AGAP) from the venom of the Chinese scorpion Buthus martensii Karsch was obtained by polymerase chain reaction (PCR) according to its cDNA sequence and expressed in Escherichia coli. While most of the recombinant AGAP was expressed in the form of insoluble inclusion body. The recombinant AGAP was purified to homogeneity by metal chelating affinity chromatography. Pharmaceutical tests showed that the recombinant AGAP has both analgesic and antitumor activities on mice.
Liu
Yan-Feng
YF
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, PR China.
Ma
Run-Lin
RL
Wang
Si-Ling
SL
Duan
Zi-Yuan
ZY
Zhang
Jin-Hai
JH
Wu
Li-Jun
LJ
Wu
Chun-Fu
CF
D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002846:Chromatography, Affinity; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D005822:Genetic Vectors; D051379:Mice; D009368:Neoplasm Transplantation; D010455:Peptides / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012604:Scorpion Venoms / Q000737:chemistry* / Q000494:pharmacology; D012605:Scorpions; D014407:Tumor Cells, Cultured
D000700:Analgesics; D000970:Antineoplastic Agents; D018076:DNA, Complementary; D010455:Peptides; D011994:Recombinant Proteins; D012604:Scorpion Venoms; C477804:antitumor-analgesic peptide, Buthus martensii