Extracellular acidic polysaccharide production by a two-membered bacterial coculture.
A two-membered coculture of strains KYM-7 and KYM-8, identified as Cellulomonas cellulans and Agrobacterium tumefaciens, respectively, produced a large amount of an extracellular polysaccharide, designated APK-78, from starch. Each strain in pure culture produced only very little amount of polysaccharide from starch; the coexistence of the two strains from the early stage of cultivation was indispensable for a large amount of polysaccharide to be produced. The polysaccharide APK-78 was acidic and composed of glucose, galactose, succinic acid, and pyruvic acid with a molar ratio of 8.1:1.0:1.7:1.0, indicating that it is a succinoglycan type of polysaccharide.
Kurata
Shinya
S
Kankyo Engineering Corporation, 1 Kimitsu, Kimitsu-shi, Chiba 299-1141, Japan.
Yamada
Kazutaka
K
Takatsu
Kyoko
K
Hanada
Satoshi
S
Koyama
Osamu
O
Yokomaku
Toyokazu
T
Kamagata
Yoichi
Y
Kanagawa
Takahiro
T
Kurane
Ryuichiro
R
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D040143:Cellulomonas / Q000235:genetics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D015169:Colony Count, Microbial; D003470:Culture Media; D004269:DNA, Bacterial / Q000096:biosynthesis; D009682:Magnetic Resonance Spectroscopy; D010802:Phylogeny; D011134:Polysaccharides / Q000096:biosynthesis* / Q000302:isolation & purification; D011809:Quinones / Q000378:metabolism; D013213:Starch
D003470:Culture Media; D004269:DNA, Bacterial; D011134:Polysaccharides; D011809:Quinones; D002244:Carbon; D013213:Starch