Megadose transplantation of highly purified haploidentical stem cells: current results and future prospects.
The transplantation of megadoses of haploidentical hematopoietic stem cells is for a number of children with malignant or nonmalignant diseases the only curative approach. In order to prevent severe GvHD, the removal of T lymphocytes from the stem cell graft either by positive selection of CD34+ stem cells or by negative depletion of CD3+ T lymphocytes is necessary. We present the results obtained so far by CD34+ positive selection and discuss new techniques of graft engineering which might hopefully further improve the outcome of haploidentical stem cell transplantation.
Handgretinger
Rupert
R
Division of Stem Cell Transplantation, St Jude Children's Research Hospital, 332 N. Lauderdale Street, Memphis, TN 38105, USA. Rupert.Handgretinger@stjude.org
Klingebiel
Thomas
T
Lang
Peter
P
Gordon
Paul
P
Niethammer
Dietrich
D
D018952:Antigens, CD34 / Q000302:isolation & purification; D005434:Flow Cytometry; D006086:Graft vs Host Disease / Q000276:immunology / Q000517:prevention & control; D006239:Haplotypes; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D008212:Lymphocyte Depletion; D013601:T-Lymphocytes / Q000276:immunology
D018952:Antigens, CD34