Effect of trichloroethylene (TCE) inhalation on biotransformation enzymes of rat lung and liver.
Trichloroethylene (TCE) is widely used as an industrial solvent and cleaning fluid. In the present study the toxic effects of TCE inhalation on pulmonary and hepatic biotransformation enzymes in rats have been investigated by assay of aniline hydroxylase (AH), aminopyrine-N-demethylase (APD), benzo-a-pyrene hydroxylase (BH) and glutathione-s-transferase (GST) activities and glutathione (GSH) contents in liver as well as lungs of exposed animals. In both organs phase I and phase II drug metabolizing enzymes have been found to be increased along with decrease in GSH contents following TCE inhalation. Pulmonary as well as hepatic MFO's seem to be activated by inhaled TCE probably in an attempt for its rapid detoxification and reduced glutathione is used during its biotransformation.
Kumar
Pradeep
P
Inhalation Toxicology Division, Animal House, Industrial Toxicology Research Centre, P. O. Box 80, M. G. Marg, Lucknow-226 001, India.
Prasad
A K
AK
Mani
U
U
Maji
B K
BK
Dutta
K K
KK
D000280:Administration, Inhalation; D000633:Aminopyrine N-Demethylase / Q000494:pharmacology*; D000815:Aniline Hydroxylase / Q000494:pharmacology*; D000818:Animals; D001579:Benzopyrene Hydroxylase / Q000494:pharmacology*; D005978:Glutathione / Q000032:analysis; D005982:Glutathione Transferase / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000008:administration & dosage / Q000009:adverse effects*; D014241:Trichloroethylene / Q000008:administration & dosage / Q000009:adverse effects*
D012997:Solvents; D014241:Trichloroethylene; D000815:Aniline Hydroxylase; D001579:Benzopyrene Hydroxylase; D000633:Aminopyrine N-Demethylase; D005982:Glutathione Transferase; D005978:Glutathione