title: Repair of 8 oxoguanine in mammalian cells expressing the Drosophila S3 ribosomal/repair protein.
abstract: 8-oxo-7,8-dihydroguanine (8-oxoG) is a potent mutagenic lesion that forms at elevated levels in cellular DNA and is repaired with low efficiency in human cells. Unlike its human counterpart, the Drosophila S3 ribosomal/repair protein is endowed with a vigorous 8 oxoG repair activity that is associated to beta,delta-elimination AP lyase activity. We have recently observed that pure GST-tagged Drosophila S3 protein can significantly accelerate the in vitro repair of 8 oxoG performed by human and mouse cell extracts [Cappelli et al., unpublished data]. In this work, we have transfected Chinese hamster cells with mammalian expression vectors containing the Drosophila S3 cDNA. The cells synthesized both S3 mRNA and protein but no improved repair of 8 oxoguanine was observed. Factors important for the proper expression of Drosophila genes in mammalian cells are discussed.
authors.affiliation: DNA Repair Unit, Mutagenesis Laboratory, Istituto Nazionale Ricerca Cancro, Genova, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Cappelli
authors.forename: Andrea
authors.initials: A
authors.lastname: D'Osualdo
authors.forename: Massimo
authors.initials: M
authors.lastname: Bogliolo
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.forename: Mark R
authors.initials: MR
authors.lastname: Kelley
authors.forename: Guido
authors.initials: G
authors.lastname: Frosina
mesh_terms: D000818:Animals; D016466:CHO Cells; D003001:Cloning, Molecular; D016384:Consensus Sequence; D006224:Cricetinae; D004260:DNA Repair / Q000235:genetics*; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics*; D005822:Genetic Vectors; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D029721:Drosophila Proteins; D012269:Ribosomal Proteins; C044152:ribosomal protein S3; C024829:8-hydroxyguanine; D006147:Guanine