title: Experimental Van Deemter plots of shear-driven liquid chromatographic separations in disposable microchannels.
abstract: We present a new stationary phase coating method, yielding a monolayer of densely arrayed porous HPLC beads (d(p)=4 microm) for use in a disposable shear-driven flow LC system. The system is inherently suited for whole-column detection through the small voids between the individual particles of the layer. The chromatographic performance of the system has been characterized by performing a series of coumarin dye separation experiments (reversed-phase mode) and by measuring the theoretical plate height as a function of the mobile phase velocity. The resulting Van Deemter curve, yielding a value of about 90,000 plates/m near the u=u(opt) velocity, shows good agreement with the theoretical expectations, and hence constitutes the first full validation of the theory of shear-driven chromatography.
authors.affiliation: Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium. nvervoor@vub.ac.be
authors.forename: Nico
authors.initials: N
authors.lastname: Vervoort
authors.forename: David
authors.initials: D
authors.lastname: Clicq
authors.forename: Gino V
authors.initials: GV
authors.lastname: Baron
authors.forename: Gert
authors.initials: G
authors.lastname: Desmet
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D008855:Microscopy, Electron, Scanning