title: [Antibiotic susceptibility and beta-lactamase activity of pathogenic microbes isolated in Miyagi Prefecture between December in 1999 and February in 2000].
abstract: We determined beta-lactamase activity and antimicrobial susceptibility of 556 strains consisting of 10 species isolated in four medical institutions and one microbiological laboratory of Miyagi Prefecture in Japan between December in 1999 and February in 2000. beta-Lactamase determined by nitrocefin method was positive in 68% of S. aureus, in 15% of H. influenzae and in 100% of M. catarrhalis. Penicillinase/cephalosporinase determined by acidometry was positive in 9%/10% of E. coli, in 17%/2% of K. pneumoniae, in 16%/58% of E. cloacae, in 43%/78% of S. marcescens, and in 4%/32% of P. aeruginosa, respectively. Of a total of 298 strains of Enterobacteriaceae and P. aeruginosa, 25 strains (14 strains of E. coli, 10 strains of K. pneumoniae and one strain of S. marcescens) produced class A beta-lactamase, two strains of E. cloacae produced class B beta-lactamase, and 12 strains (one strain of E. coli, four strains of E. cloacae, six strains of S. marcescens and one strain of P. aeruginosa) produced class C beta-lactamase. According to NCCLS standard, three strains (one strain of E. coli and two strains of K. pneumoniae) of ESBL-positive microbes were detected. beta-Lactamase-negative ampicillin-resistant (BLNAR) strains of H. influenzae were found in 10/40 (25.0%) of the strains tested.
authors.affiliation: Department of Respiratory Oncology and Molecular Medicine, Institute of Development, Aging and Cancer, Tohoku University.
authors.forename: Akira
authors.initials: A
authors.lastname: Watanabe
authors.forename: Satoko
authors.initials: S
authors.lastname: Yasui
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Murayama
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tokue
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takahashi
authors.forename: Tohru
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Kazunori
authors.initials: K
authors.lastname: Gomi
authors.forename: Takao
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Fujimura
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nukiwa
authors.forename: Nobuko
authors.initials: N
authors.lastname: Sato
authors.forename: Michiko
authors.initials: M
authors.lastname: Saito
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Chiba
authors.forename: Hisao
authors.initials: H
authors.lastname: Oikawa
authors.forename: Toshio
authors.initials: T
authors.lastname: Sato
mesh_terms: D004755:Enterobacteriaceae / Q000201:enzymology*; D007564:Japan; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001618:beta-Lactamases