Analytical model for determination of parameters of helical structures in solution by small angle scattering: comparison of RecA structures by SANS.
The filament structures of the self-polymers of RecA proteins from Escherichia coli and Pseudomonas aeruginosa, their complexes with ATPgammaS, phage M13 single-stranded DNA (ssDNA) and the tertiary complexes RecA::ATPgammaS::ssDNA were compared by small angle neutron scattering. A model was developed that allowed for an analytical solution for small angle scattering on a long helical filament, making it possible to obtain the helical pitch and the mean diameter of the protein filament from the scattering curves. The results suggest that the structure of the filaments formed by these two RecA proteins, and particularly their complexes with ATPgammaS, is conservative.
Lebedev
D V
DV
Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russia.
Baitin
D M
DM
Islamov
A Kh
AKh
Kuklin
A I
AI
Shalguev
V Kh
VKh
Lanzov
V A
VA
Isaev-Ivanov
V V
VV
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry; D001665:Binding Sites; D004277:DNA, Single-Stranded / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D029968:Escherichia coli Proteins / Q000737:chemistry; D008962:Models, Theoretical; D017433:Protein Structure, Secondary; D011550:Pseudomonas aeruginosa / Q000737:chemistry; D011938:Rec A Recombinases / Q000737:chemistry*; D012542:Scattering, Radiation; D013057:Spectrum Analysis
D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; D029968:Escherichia coli Proteins; C022571:adenosine 5'-O-(3-thiotriphosphate); D000255:Adenosine Triphosphate; D011938:Rec A Recombinases