[Transfection of the exogenous PTEN-induced growth inhibition of the highly metastatic mucoepidermoid carcinoma cell line M3SP2 in vitro].
OBJECTIVE
The purpose of this study was to evaluate the effects of the exogenous phosphatase and tensin homology deleted on chromosome 10 (PTEN) gene on in vitro growth of the highly metastatic mucoepidemoid carcinoma cell line M3SP2.


METHODS
The growth of the exogenous PTEN transfected mucoepidemoid carcinoma cells M3SP2-PTEN gene was studied by analyzing cell growth curves, mitosis index and clone formation efficiency and compared with its parental cell line M3SP2 and the vector pBabepuro-transfected cell line M3SP2-pBp.


RESULTS
The doubling time (h) of M3SP2, M3SP2-pBp and M3SP2-PTEN were 24.50, 24.76 and 31.74; the mitosis index (@1000) were 53.0 +/- 6.20, 49.0 +/- 5.24 and 16.2 +/- 3.2; the clone formation efficiency (%) were 37.37, 35.01 and 10.40, respectively. The M3SP2-PTEN cells also revealed 57.05%-71.46% inhibition of growth from day 3 to 7 and 65%-72% inhibition of clone formation compared with the parental cells.


CONCLUSIONS
These data provide evidence that the exogenous wild-type PTEN have remarkably inhibitory effects on in vitro proliferation of the highly metastatic mucoepidermoid carcinoma cell line M3SP2.
Liu
Bin
B
Department of Oral Biology, Stomatological College, Fourth Military Medical University.
Wu
Junzheng
J
Li
Yan
Y
Sun
An
A
Ye
Weili
W
D018277:Carcinoma, Mucoepidermoid / Q000235:genetics / Q000473:pathology*; D002455:Cell Division; D002999:Clone Cells; D003001:Cloning, Molecular; D015316:Genetic Therapy; D006801:Humans; D051059:PTEN Phosphohydrolase; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D012468:Salivary Gland Neoplasms / Q000235:genetics / Q000473:pathology*; D014162:Transfection; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D025521:Tumor Suppressor Proteins; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human