Rapid method for the fractionation of nuclear proteins and their complexes by batch elution from hydroxyapatite.
A new procedure for the separation and purification of nuclear proteins and their complexes by batch elution from hydroxyapatite is presented. This method allows to isolate such proteins with different basic character faster and more efficiently than procedures using column chromatography, while showing high selectivity, sensitivity, simplicity, mild conditions of purification, reproducibility and protein stability.
Zagariya
A
A
Department of Biochemistry and Molecular Genetics, University of Alabama, UAB Station, Birmingham, AL 35294, USA.
Khrapunov
S
S
Zacharias
W
W
D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods; D017886:Durapatite; D006657:Histones / Q000302:isolation & purification; D009687:Nuclear Proteins / Q000302:isolation & purification*
D006657:Histones; D009687:Nuclear Proteins; D017886:Durapatite