title: Expression level of integrin alpha 5 on tumour cells affects the rate of metastasis to the kidney.
abstract: Tumour metastasis is known clinically to have organ specificity. We hypothesised that integrins might be involved in determining the organ specificity of tumour metastasis. Here, we report the results of spontaneous metastasis tested in nude mice that were inoculated with Chinese hamster ovary (CHO) cells expressing integrin alpha 5 beta 1 at various levels. The growth of the primary tumour inversely correlated with the alpha 5 expression level on CHO cells, which is consistent with a previous report (Schreiner et al, 1991). The rates of pulmonary, lymph node, and adrenal metastases that developed in nude mice were not related to changes of the alpha 5 expression level on CHO cells. Kidney metastasis developed in 40% of nude mice inoculated with alpha 5B2 cells (CHO cells overexpressing alpha 5) and in 20% of mice with CHO-K1 cells (CHO cells expressing native alpha 5), whereas inoculation with CHO-B2 cells (alpha 5-defective mutants) and alpha 5CHO cells with the highest expression of alpha 5 did not lead to development of kidney metastasis. Furthermore, alpha 5CHO, which shows the slowest growth of these cell types, did not lead to primary tumours in nude mice. These findings suggest that there is an appropriate level of alpha 5 expression on tumour cells that leads to metastasis. Microscopic observations revealed that micrometastasis in the kidney was formed in glomeruli. An adhesion assay using frozen sections of the kidney demonstrated that alpha 5B2 cells, but not CHO-B2 cells, effectively adhered to glomeruli. Kidney metastasis in vivo and the adhesion of alpha 5B2 to glomeruli shown ex vivo were significantly suppressed by the administration of GRGDS peptide. Finally, we conclude that the interaction of alpha 5 beta 1 on tumour cells with fibronectin in kidney glomeruli is involved in kidney metastasis and that the tumour has appropriate levels of integrins crucial for metastasis.
authors.affiliation: Division of Structutral Cell Biology, Nara Institute of Science and Technology, Ikoma, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Tani
authors.forename: S
authors.initials: S
authors.lastname: Higashiyama
authors.forename: N
authors.initials: N
authors.lastname: Kawaguchi
authors.forename: J
authors.initials: J
authors.lastname: Madarame
authors.forename: I
authors.initials: I
authors.lastname: Ota
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
authors.forename: Y
authors.initials: Y
authors.lastname: Ohoka
authors.forename: S
authors.initials: S
authors.lastname: Shiosaka
authors.forename: Y
authors.initials: Y
authors.lastname: Takada
authors.forename: N
authors.initials: N
authors.lastname: Matsuura
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000378:metabolism / Q000473:pathology / Q000556:secondary; D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion; D002455:Cell Division; D002465:Cell Movement; D006224:Cricetinae; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D039482:Integrin alpha5 / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D007680:Kidney Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000556:secondary*; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology / Q000556:secondary; D008207:Lymphatic Metastasis / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000098:blood supply* / Q000235:genetics / Q000473:pathology; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology; D017469:Receptors, Fibronectin / Q000378:metabolism; D014162:Transfection
chemical_list: D005353:Fibronectins; D039482:Integrin alpha5; D010446:Peptide Fragments; D017469:Receptors, Fibronectin