title: Araçatuba virus: a vaccinialike virus associated with infection in humans and cattle.
abstract: We describe a vaccinialike virus, Araçatuba virus, associated with a cowpoxlike outbreak in a dairy herd and a related case of human infection. Diagnosis was based on virus growth characteristics, electron microscopy, and molecular biology techniques. Molecular characterization of the virus was done by using polymerase chain reaction amplification, cloning, and DNA sequencing of conserved orthopoxvirus genes such as the vaccinia growth factor (VGF), thymidine kinase (TK), and hemagglutinin. We used VGF-homologous and TK gene nucleotide sequences to construct a phylogenetic tree for comparison with other poxviruses. Gene sequences showed 99% homology with vaccinia virus genes and were clustered together with the isolated virus in the phylogenetic tree. Araçatuba virus is very similar to Cantagalo virus, showing the same signature deletion in the gene. Araçatuba virus could be a novel vaccinialike virus or could represent the spread of Cantagalo virus.
authors.affiliation: Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.
authors.forename: Giliane
authors.initials: G
authors.lastname: de Souza Trindade
authors.forename: Flávio Guimarães
authors.initials: FG
authors.lastname: da Fonseca
authors.forename: João Trindade
authors.initials: JT
authors.lastname: Marques
authors.forename: Maurício Lacerda
authors.initials: ML
authors.lastname: Nogueira
authors.forename: Luiz Claudio Nogueira
authors.initials: LC
authors.lastname: Mendes
authors.forename: Alexandre Secorun
authors.initials: AS
authors.lastname: Borges
authors.forename: Juliana Regina
authors.initials: JR
authors.lastname: Peiró
authors.forename: Edviges Maristela
authors.initials: EM
authors.lastname: Pituco
authors.forename: Cláudio Antônio
authors.initials: CA
authors.lastname: Bonjardim
authors.forename: Paulo César Peregrino
authors.initials: PC
authors.lastname: Ferreira
authors.forename: Erna Geessien
authors.initials: EG
authors.lastname: Kroon
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D021821:Communicable Diseases, Emerging / Q000473:pathology; D005814:Genes, Viral / Q000235:genetics; D006801:Humans; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D018155:Orthopoxvirus / Q000235:genetics; D010802:Phylogeny; D016415:Sequence Alignment; D014615:Vaccinia / Q000473:pathology / Q000662:veterinary / Q000821:virology*; D014616:Vaccinia virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics; D014766:Viremia / Q000453:epidemiology
chemical_list: C088596:A-type inclusion protein, poxvirus; D014764:Viral Proteins; D015678:Viral Structural Proteins