title: Kindling status in sprague-dawley rats induced by pentylenetetrazole: involvement of a critical development period.
abstract: Kindled seizures are widely used as a model for epileptogenesis. Although the achievement of kindling criterion is known to require time to develop, the precise developmental period has not been identified. We now report that optimal achievement of the kindling criterion in the Sprague-Dawley rat is associated with a critical inter-stimulus interval of 24 to 26 days. We show that highly efficient kindling can be achieved with only two subconvulsive doses of pentylenetetrazole so long as they are given 25 days apart. Using Northern blot hybridization we show that the increased seizure susceptibility at 25 days coincides with an increased expression of the plasticity-associated proteins, growth-associated protein-43 (GAP-43), microtubule-associated protein 1B (MAP1B), and tissue plasminogen activator (tPA) mRNAs in the hippocampus. By in situ hybridization and immunocytochemistry on tissue sections, we also show an increased expression for GAP-43 in the polymorphic layer of the dentate gyrus, mossy fibers, and pyramidal cells in the CA3 region of the hippocampus. The demonstration of a long, defined developmental interval for inducing the kindling criterion should enable a dissection of the cellular and genetic events underlying this phenomenon in the rat.
authors.affiliation: Department of Neurology, Ernst-Moritz-Arndt-University, Greifswald, Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Schmoll
authors.forename: Irina
authors.initials: I
authors.lastname: Badan
authors.forename: Gisela
authors.initials: G
authors.lastname: Grecksch
authors.forename: Lary
authors.initials: L
authors.lastname: Walker
authors.forename: Christoff
authors.initials: C
authors.lastname: Kessler
authors.forename: Aurel
authors.initials: A
authors.lastname: Popa-Wagner
mesh_terms: D000818:Animals; D015415:Biomarkers; D015152:Blotting, Northern; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D004827:Epilepsy / Q000235:genetics / Q000473:pathology; D019922:GAP-43 Protein / Q000235:genetics; D005786:Gene Expression Regulation; D007696:Kindling, Neurologic / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics; D010433:Pentylenetetrazole / Q000494:pharmacology*; D015347:RNA Probes; D018075:RNA, Complementary / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D010959:Tissue Plasminogen Activator / Q000235:genetics
chemical_list: D015415:Biomarkers; D019922:GAP-43 Protein; D008869:Microtubule-Associated Proteins; D015347:RNA Probes; D018075:RNA, Complementary; C085284:microtubule-associated protein 1B; D010959:Tissue Plasminogen Activator; D010433:Pentylenetetrazole