title: [Study on expression of beta-NGF in myoblasts transfected by PSVCEP NGF-CAT with Lipofect AMINE].
abstract: OBJECTIVE
  To gain an understanding of the expression of beta-NGF in myoblasts transfected by PSVCEP NGF-CAT and the transfecting efficiency of Lipofect AMINE.
  
  
  METHODS
  PSVCEP NGF-CAT was delivered into cultured myoblasts by Lipofect AMINE. Expression of beta-NGF in the transfected myoblasts was detected by immunocytochemistry.
  
  
  RESULTS
  Lipofect AMINE was evidently engulfed by myoblasts at the 6th hour; about 40% myoblasts could be detected with the expression of beta-NGF by immunocytochemistry at the 48th hour.
  
  
  CONCLUSIONS
  PSVCEP NGF-CAT can express beta-NGF in cultured myoblasts, and Lipofect AMINE is a convenient vector for gene transfection with simplicity and efficacy.
authors.affiliation: Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Boyong
authors.initials: B
authors.lastname: Mao
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Liu
authors.forename: Junjie
authors.initials: J
authors.lastname: Chen
authors.forename: Ruohan
authors.initials: R
authors.lastname: Wang
authors.forename: Yongzhong
authors.initials: Y
authors.lastname: Cheng
authors.forename: Lida
authors.initials: L
authors.lastname: Gao
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.forename: Shengfu
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D010714:Phosphatidylethanolamines; D051381:Rats; D017208:Rats, Wistar; D014162:Transfection
chemical_list: D010714:Phosphatidylethanolamines; C020888:1,2-dielaidoylphosphatidylethanolamine; D020932:Nerve Growth Factor