Zea mays ZmMybst1 cDNA, encodes a single Myb-repeat protein with the VASHAQKYF motif.
A cDNA clone from a 4 DAP dissected maize embryo sac encoding a novel Zea mays single-repeat Myb protein is reported here. This full-length cDNA contains an ORF of 948 bp. The gene ZmMybst1 contains two introns (1166 and 706 bp) and is a single copy gene. The ZmMybst1 protein shares high sequence identity with the potato Mybst1 protein (58%). Northern blot, RT-PCR and electronic northern analysis shows that ZmMybst1 is expressed in endosperm between 4 and 30 DAP, coinciding with the period of aleurone cell differentiation and development.
Mercy
Inderjit Singh
IS
PO Box 5051, Agricultural University of Norway, 1432 Aas, Norway. Inderjit.mercy@bio.uio.no
Meeley
Robert B
RB
Nichols
Scott E
SE
Olsen
Odd-Arne
OA
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000254:growth & development / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D018076:DNA, Complementary; D010940:Plant Proteins; D020598:Proto-Oncogene Proteins c-myb; D012333:RNA, Messenger