Hemese, a hemocyte-specific transmembrane protein, affects the cellular immune response in Drosophila.
We have identified a previously undescribed transmembrane protein, Hemese, from Drosophila melanogaster blood cells (hemocytes), by using a monoclonal pan-hemocyte antibody. Heavy glycosylation is suggested by the heterogeneous size distribution, ranging between 37 and 70 kDa. Hemese expression is restricted to the cell surfaces of hemocytes of all classes, and to the hematopoietic organs. The sequence of the corresponding gene, Hemese (He), predicts a glycophorin-like protein of 15 kDa, excluding an N-terminal signal peptide, with a single hydrophobic transmembrane region. The extracellular region consists mainly of Ser/Thr-rich sequence of low complexity, with several potential O-glycosylation sites. Hemese contains phosphotyrosine and the cytoplasmic region has potential phosphorylation sites, suggesting an involvement in signal transduction. Depletion of Hemese by RNA interference has no obvious effect under normal conditions, but the cellular response to parasitic wasps is much enhanced. This finding indicates that Hemese plays a modulatory role in the activation or recruitment of the hemocytes.
Kurucz
Eva
E
Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, P.O Box 521, H-6701 Szeged, Hungary. Umeå Centre for Molecular Pathogenesis, By. 6L, Umeå University, S-901 87 Umeå, Sweden.
Zettervall
Carl-Johan
CJ
Sinka
Rita
R
Vilmos
Peter
P
Pivarcsi
Andor
A
Ekengren
Sophia
S
Hegedüs
Zoltán
Z
Ando
Istvan
I
Hultmark
Dan
D
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism*; D002469:Cell Separation; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins / Q000737:chemistry* / Q000502:physiology*; D005434:Flow Cytometry; D015723:Gene Library; D006031:Glycosylation; D049452:Green Fluorescent Proteins; D006434:Hemocytes / Q000378:metabolism*; D006825:Hybridomas; D007150:Immunohistochemistry; D008164:Luminescent Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000502:physiology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D010587:Phagocytosis; D010766:Phosphorylation; D011233:Precipitin Tests; D017434:Protein Structure, Tertiary; D012313:RNA / Q000378:metabolism; D034622:RNA Interference; D015398:Signal Transduction; D014443:Tyrosine / Q000378:metabolism
D018076:DNA, Complementary; D029721:Drosophila Proteins; C473331:He protein, Drosophila; D008164:Luminescent Proteins; D008565:Membrane Proteins; D010455:Peptides; D049452:Green Fluorescent Proteins; D014443:Tyrosine; D012313:RNA