title: Colony-forming capacity of porcine liver epithelial cells in culture.
abstract: Previously, we reported the presence of certain nonparenchymal epithelial cells (NPECs) in adult porcine livers that demonstrate differentiation patterns including an emergence of duct-like structures (DLSs) in the colonies. In the present study, we examined the effect of supplements to the NAIR-1 medium (Dulbecco modified Eagle medium [DMEM]-F12 containing 5% fetal bovine serum [FBS] and 11 supplements) used in these cultures on formation of DLSs-emerged colonies (type I colonies). No type I colonies were observed in the cultures of the nonparenchymal cell fraction when Roswell Park Memorial Institute-1640 medium or DMEM-F12 (1:1) supplemented with 5% FBS was used as the culture medium. NAIR-1 medium without each component did not produce any significant results. No type I colonies were formed when epidermal growth factor, and hydrocortisone and insulin mixture (A) or nicotinamide and l-ascorbic acid phosphate magnesium salt (Asc2P) mixture (B) was added to the DMEM-F12 medium supplemented with 5% FBS. However, when a combination of A and B was added, colonies were formed at a significant level. Together, the number of type I colonies was increased in the combination of A and B containing a higher concentration of Asc2P. We conclude that NPECs need a mixture of Asc2P and other components as supplements for type 1 colony formation.
authors.affiliation: Kohno Clinical Medicine Research Institute, Tokyo, Japan.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Tokiwa
authors.forename: Taisuke
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Junko
authors.initials: J
authors.lastname: Kano
authors.forename: Masayuki
authors.initials: M
authors.lastname: Noguchi
authors.forename: Shin
authors.initials: S
authors.lastname: Enosawa
authors.forename: Minoru
authors.initials: M
authors.lastname: Kohno
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002455:Cell Division; D004847:Epithelial Cells / Q000166:cytology; D008099:Liver / Q000166:cytology*; D013552:Swine