The effects of prostaglandin A2 on cell growth, cell cycle status and apoptosis induction in HeLa and MCF-7 cells.
The effects of 20 microg/ml exogenous prostaglandin A(2) (PGA(2)) were evaluated on cell numbers in HeLa (human epithelial cervix carcinoma) and MCF-7 (human breast carcinoma) cells. In HeLa cells, PGA(2) reduced cell numbers significantly to 75% after 24 h (P < 0.05) and exposure of 48 h decreased cell numbers to 61% (P < 0.05) of the control. In MCF-7 cells, PGA(2) significantly reduced cell numbers to 48% after 24 h and to 20% after 48 h, compared to vehicle-treated control cells (P < 0.05). The anti-mitogenic effects were confirmed by morphological studies conducted after 48 h of exposure to PGA(2), when optimal effects were observed. HeLa and MCF-7 cells exposed to PGA(2), showed chromatin aggregation, cell membrane blebbing and uneven distribution of chromosomes. Cell cycle progression analysis of HeLa and MCF-7 cells, showed an increase in DNA content preceding the G(0)/G(1) peak after 48 h of exposure, which is indicative of apoptotic body formation.
Joubert
Annie M
AM
Department of Physiology, University of Pretoria, PO Box 2034, Pretoria 0001, South Africa. ajoubert@postillion.up.ac.za
Panzer
Annie
A
Bianchi
Pepita C
PC
Lottering
Mona Liza
ML
D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000473:pathology*; D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D005260:Female; D006367:HeLa Cells / Q000187:drug effects / Q000473:pathology; D006801:Humans; D011454:Prostaglandins A / Q000494:pharmacology*
D011454:Prostaglandins A; C100008:prostaglandin A2