title: Improved arachidonic acids production from the fungus Mortierella alpina by glutamate supplementation.
abstract: The effect of various concentrations of glutamate on arachidonic acid (AA) production from Mortierella alpina in shaker flask culture was studied. Glutamate supplementation promoted Mortierella growth, accelerated substrate metabolism, and increased AA production, and a concentration of 0.8 g/l glutamate resulted in the greatest AA yield (1.41 g/l). In 10 l airlift stirred fermenter culture, AA yield in the cultures exposed to 0.8 g/l glutamate was also greater than that in the control (0.56 g/l).
authors.affiliation: School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China. yulongjiang@mail.hust.edu.cn
authors.forename: L J
authors.initials: LJ
authors.lastname: Yu
authors.forename: W M
authors.initials: WM
authors.lastname: Qin
authors.forename: W Z
authors.initials: WZ
authors.lastname: Lan
authors.forename: P P
authors.initials: PP
authors.lastname: Zhou
authors.forename: M
authors.initials: M
authors.lastname: Zhu
mesh_terms: D016718:Arachidonic Acid / Q000096:biosynthesis*; D018533:Biomass; D003470:Culture Media; D005285:Fermentation / Q000187:drug effects; D018698:Glutamic Acid / Q000378:metabolism* / Q000494:pharmacology*; D020094:Mortierella / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*
chemical_list: D003470:Culture Media; D016718:Arachidonic Acid; D018698:Glutamic Acid