Use of an open-reading frame-specific Campylobacter jejuni DNA microarray as a new genotyping tool for studying epidemiologically related isolates.
Findings from use of an open-reading frame-specific Campylobacter jejuni DNA microarray to investigate genetic diversity among clinical isolates associated with 5 independent clusters of infection were compared with data from random amplified polymeric DNA (RAPD) and Penner serotyping analyses. The DNA microarray provides a highly specific epidemiological typing tool for analysis of C. jejuni isolates and reveals both divergent and highly conserved gene classes among isolates.
Leonard
Edward E
EE
Department of Microbiology and Immunology, Division of Infectious Diseases, Stanford University, Stanford, California 94305, USA. eleonard@stanford.edu
Takata
Tohru
T
Blaser
Martin J
MJ
Falkow
Stanley
S
Tompkins
Lucy S
LS
Gaynor
Erin C
EC
D000818:Animals; D002169:Campylobacter Infections / Q000453:epidemiology; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002417:Cattle; D004269:DNA, Bacterial; D020869:Gene Expression Profiling; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D019105:Random Amplified Polymorphic DNA Technique
D004269:DNA, Bacterial