Hsp60 specific antibodies in egg yolks from laying hens naturally infected with Salmonella enterica subspecies enterica serovar Enteritidis.
Heat shock protein (Hsp) 60 of Salmonella appears to be involved in pathogenesis of infectious processes and host immune responses. Eggs of laying hens from two Salmonella Enteritidis naturally infected flocks (I--acute outbreak of infection; II--occasional bacteria excretion) and one control flock (III) were tested for the presence of yolk antibodies (IgY) against Hsp60 by applying enzyme-linked immunosorbent assay (ELISA). The levels of specific immunoglobulins were related to those against lipopolysaccharide (LPS) and flagellin. the antigens of the established immunological importance in S. Enteritidis infections. Within flock III, the antibody concentrations were consistently low. Elevated levels were detected in eggs from two infected flocks. Levels of specific IgY measured for flock I were higher than those in flock II; the greatest difference was observed for anti-Hsp60. This report indicates a probable important role of Hsp60 as a target of the hens' immune response, especially during the acute phase of S. Enteritidis infection.
Dera-Tomaszewska
Bozena
B
National Salmonella Centre, Department of Immunology, Institute of Maritime and Tropical Medicine, Powstania Styczniowego 9B, 81-519 Gdynia, Poland. bodeto@immt.gdynia.pl
Wysocki
Jan
J
Kunikowska
Danuta
D
Dziadziuszko
Halina
H
Głośnicka
Renata
R
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D018834:Chaperonin 60 / Q000276:immunology*; D002645:Chickens; D004530:Egg Yolk / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D011201:Poultry Diseases / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology; D012477:Salmonella enteritidis / Q000276:immunology*
D000907:Antibodies, Bacterial; D018834:Chaperonin 60