title: Impact of hypercholesterolemia on acidosis-induced coronary microvascular dilation.
abstract: An increase in coronary flow conductance during acidosis is an important compensatory mechanism in various diseased conditions. On the other hand, hypercholesterolemia causes microvascular dysfunction as well as macrovascular disorders. We investigated the impact of hypercholesterolemia on the coronary microvascular response to acidosis. Coronary arterioles (< 150 microm) isolated from rabbit hearts were cannulated to micropipettes in a vessel chamber and the microvascular responses were observed. After preconstriction was established, the extravascular pH was gradually reduced from 7.4 to 7.0. The effects of glibenclamide, ATP-sensitive K(+) (K(ATP)) channel blocker, (1 microM, n = 4) or pertussis toxin (100 ng/mL, n = 7) on the acidosis-induced microvascular responses were examined. In another set of experiments, rabbits were randomly assigned to normal chow (NC group, n = 18) or high cholesterol (2 %) diet (HC group, n = 20). After 8 weeks of feeding, the responses of isolated coronary arterioles to acidosis, ADP, nitroprusside, and levcromakalim were examined in the two groups. Coronary arterioles significantly dilated as the pH was reduced and the dilation was significantly inhibited by glibenclamide or pertussis toxin. Acidosis-induced dilation in the HC group was significantly attenuated compared to the NC group (36.5 +/- 2.1 % vs 73.7 +/- 4.8 % at pH = 7.0 P < 0.05). There were no significant differences in the dilations by ADP, nitroprusside and levcromakalim between the two groups. In conclusion, acidosis-induced dilation of rabbit coronary arterioles is mediated by the activation of the pertussis toxin-sensitive G protein and K(ATP) channels, and the dilation of coronary arterioles is impaired in hypercholesterolemia. The impairment occurs upstream of K(ATP) channel opening.
authors.affiliation: Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku Sendai 980-8574, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Shioiri
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Komaru
authors.forename: Kouichi
authors.initials: K
authors.lastname: Sato
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Takahashi
authors.forename: Satoru
authors.initials: S
authors.lastname: Takeda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kanatsuka
authors.forename: Jun
authors.initials: J
authors.lastname: Watanabe
authors.forename: Kunio
authors.initials: K
authors.lastname: Shirato
mesh_terms: D000138:Acidosis / Q000150:complications; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology; D001160:Arterioles / Q000503:physiopathology; D003331:Coronary Vessels / Q000503:physiopathology*; D004108:Dilatation, Pathologic / Q000209:etiology; D004195:Disease Models, Animal; D019204:GTP-Binding Proteins / Q000378:metabolism; D005905:Glyburide / Q000494:pharmacology; D006937:Hypercholesterolemia / Q000503:physiopathology*; D008297:Male; D009924:Organ Culture Techniques; D037342:Pertussis Toxin / Q000494:pharmacology; D015221:Potassium Channels / Q000378:metabolism; D011817:Rabbits
chemical_list: D000889:Anti-Arrhythmia Agents; D015221:Potassium Channels; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D005905:Glyburide