An algorithm for identification and ranking of family-specific residues, applied to the ALDH3 family.
An algorithm for detecting amino acid residues characteristic of individual protein families from within aligned collections of paralogous sequences, and its application to the ALDH3 family versus the rest of the ALDH extended family is described. Residues illuminated by this analysis include a key intramolecular tether, a lysine that makes an intersubunit contact at the dimer interface, three residues in close association with the substrate-binding funnel, and a pair of residues suggested to participate in proton relay during the catalytic cycle.
Hempel
John
J
Department of Biological Sciences, University of Pittsburgh, Clapp Hall 301, Pittsburgh, PA 15260, USA. hempel@psc.edu
Perozich
John
J
Wymore
Troy
T
Nicholas
Hugh B
HB
D000444:Aldehyde Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D000465:Algorithms; D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism; D020134:Catalytic Domain; D008958:Models, Molecular
D000596:Amino Acids; C030863:corneal protein 54, bovine; D000444:Aldehyde Dehydrogenase