Repair of 8 oxoguanine in mammalian cells expressing the Drosophila S3 ribosomal/repair protein.
8-oxo-7,8-dihydroguanine (8-oxoG) is a potent mutagenic lesion that forms at elevated levels in cellular DNA and is repaired with low efficiency in human cells. Unlike its human counterpart, the Drosophila S3 ribosomal/repair protein is endowed with a vigorous 8 oxoG repair activity that is associated to beta,delta-elimination AP lyase activity. We have recently observed that pure GST-tagged Drosophila S3 protein can significantly accelerate the in vitro repair of 8 oxoG performed by human and mouse cell extracts [Cappelli et al., unpublished data]. In this work, we have transfected Chinese hamster cells with mammalian expression vectors containing the Drosophila S3 cDNA. The cells synthesized both S3 mRNA and protein but no improved repair of 8 oxoguanine was observed. Factors important for the proper expression of Drosophila genes in mammalian cells are discussed.
Cappelli
Enrico
E
DNA Repair Unit, Mutagenesis Laboratory, Istituto Nazionale Ricerca Cancro, Genova, Italy.
D'Osualdo
Andrea
A
Bogliolo
Massimo
M
Xu
Yi
Y
Kelley
Mark R
MR
Frosina
Guido
G
D000818:Animals; D016466:CHO Cells; D003001:Cloning, Molecular; D016384:Consensus Sequence; D006224:Cricetinae; D004260:DNA Repair / Q000235:genetics*; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics*; D005822:Genetic Vectors; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection
D018076:DNA, Complementary; D029721:Drosophila Proteins; D012269:Ribosomal Proteins; C044152:ribosomal protein S3; C024829:8-hydroxyguanine; D006147:Guanine