Microencapsulation and modification of synthetic peptides of food proteins reduces the blood pressure of spontaneously hypertensive rats.
Synthetic peptides were microencapsulated into liposomes, cycled with a disulfide bond or modified with d-phenylglycine (d-phg) at the N-terminal, and their antihypertensive effects as orally administered (0.18 mM/kg body weight) to spontaneously hypertensive rats (SHR) were measured. The microencapsulated Leu-Lys-Pro reduced significantly the systolic blood pressures of SHR by 45 mmHg and showed a prolonged duration, revealing the significant protective effect of encapsulation. d-phg-Leu-Arg-Pro showed a duration about 2 h shorter than that of the peptide without modification. In addition, cyclic Leu-Arg-Pro peptide with a disulfide bond between the N- and C-terminal amino acids reduced the systolic blood pressure of SHR by 35 mmHg and displayed a lengthy duration.
Chen
Tzy-Li
TL
Graduate Institute of Food Science & Technology, National Taiwan University, Taipei 106-17, Taiwan.
Lo
Yung-Chieh
YC
Hu
Wei-Tze
WT
Wu
Ming-Chang
MC
Chen
Shui-Ten
ST
Chang
Hung-Min
HM
D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000008:administration & dosage; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage*; D001794:Blood Pressure / Q000187:drug effects*; D002216:Captopril / Q000494:pharmacology; D004044:Dietary Proteins / Q000032:analysis*; D006868:Hydrolysis; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000008:administration & dosage / Q000737:chemistry; D051381:Rats; D011918:Rats, Inbred SHR; D013329:Structure-Activity Relationship
D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D004044:Dietary Proteins; D008081:Liposomes; D010455:Peptides; D010456:Peptides, Cyclic; D002216:Captopril