title: Genomic organization and embryonic expression of Igsf8, an immunoglobulin superfamily member implicated in development of the nervous system and organ epithelia.
abstract: Igsf8 is an immunoglobulin protein that binds to the tetraspanin molecules, CD81 and CD9. We describe the genomic organization of mouse and human Igsf8, and reveal a dynamic expression pattern during embryonic and fetal development. Igsf8 is first expressed at E9.5 in a ventral domain of the neural tube, with dorsal expression apparent at E10.5. We show that the ventral, but not the dorsal, domain of neural tube expression is dependent on Shh signaling. From E11.5, Igsf8 is expressed at the lateral edge of the ventricular zone, in early postmitotic neuroblasts, and in dorsal root and cranial ganglia. Igsf8 is also expressed in the branchial arches, dorsal pancreatic primordium, neural retina, olfactory epithelium, gut, kidney, and lung.
authors.affiliation: Neural Development Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK. j.murdoch@har.mrc.ac.uk
authors.forename: Jennifer N
authors.initials: JN
authors.lastname: Murdoch
authors.forename: Kit
authors.initials: K
authors.lastname: Doudney
authors.forename: Dianne
authors.initials: D
authors.lastname: Gerrelli
authors.forename: Noel
authors.initials: N
authors.lastname: Wortham
authors.forename: Caroline
authors.initials: C
authors.lastname: Paternotte
authors.forename: Philip
authors.initials: P
authors.lastname: Stanier
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Copp
mesh_terms: D000818:Animals; D015703:Antigens, CD; D002490:Central Nervous System / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D004848:Epithelium / Q000196:embryology* / Q000378:metabolism; D005091:Exons / Q000235:genetics; D005260:Female; D005333:Fetus; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D016678:Genome; D053823:Hedgehog Proteins; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics* / Q000378:metabolism*; D007438:Introns / Q000235:genetics; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D017933:Peripheral Nervous System / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D015534:Trans-Activators / Q000172:deficiency / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014781:Viscera / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D029961:Zebrafish Proteins
chemical_list: D015703:Antigens, CD; D053823:Hedgehog Proteins; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins; C440028:IGSF8 protein, human; D007136:Immunoglobulins; D008565:Membrane Proteins; C105201:NKX6-1 protein, human; C491328:Nkx6-1 protein, mouse; C509239:SHH protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D029961:Zebrafish Proteins