Genomic structure and inducible expression of the IL-22 receptor alpha chain in mice.
IL-22 is a newly identified member of the interferon/IL-10 family. In humans, IL-22 signals through a heteroduplex receptor consisting of IL-22R and CRF2-4/IL-10Rbeta. To investigate the physiological function of IL-22 and IL-22R, we isolated a cDNA encoding the mouse IL-22R, which has been a missing component of the functional receptor complex for mouse IL-22. Subsequently, we identified the genomic sequence of the mouse IL-22R gene by a database search. The gene consists of about 24 kb and is split into seven exons. Interestingly, intron 2 begins with a GC dinucleotide instead of the consensus GT, although otherwise the overall structure of the mouse IL-22R gene is strikingly similar to its human counterpart. The gene was mapped to mouse chromosome 4 in the region syntenic to the human IL-22R gene locus. In normal mice, IL-22R mRNA is detected at very low levels in restricted organs such as the kidney, liver, and lung. However, upon lipopolysaccharide stimulation, IL-22R mRNA expression is highly upregulated in the liver, in contrast to CRF2-4, which is expressed constitutively in a variety of tissues. Thus, the expression of the functional IL-22 receptor in the liver is regulated at the gene transcription level.
Tachiiri
A
A
Centre for the Development of Molecular Targets Drugs, Cancer Research Institute, Kanazawa University, Kanazawa, Japan.
Imamura
R
R
Wang
Y
Y
Fukui
M
M
Umemura
M
M
Suda
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002460:Cell Line; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005260:Female; D040461:Gene Components; D005786:Gene Expression Regulation; D016678:Genome; D006801:Humans; D007378:Interleukins / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D016415:Sequence Alignment; D015398:Signal Transduction; D000097043:Interleukin-22
D018076:DNA, Complementary; D007378:Interleukins; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D000097043:Interleukin-22; C415369:interleukin-22 receptor