title: Effect of polar head groups on the activity of aspartyl protease adsorbed to lipid membranes.
abstract: The proteolytic activity of an aspartyl protease of Mucor miehei was correlated with the adsorption of the protease to lipid vesicles. It was observed that the presence of phosphatidylethanolamines (PE's) in the membrane increased the enzyme activity in a 20% in the gel phase and 10% in the fluid phase. The effects of protease on the surface pressure of monolayers composed by dioleoylphosphatidylcholine (DOPC), dimyristoylphosphatidylcholine (DMPC), dimyristoyl phosphatidylethanolamine (DMPE) were measured at constant temperature as a function of the surface pressure. At low surface pressures, the major changes were induced by protease on DOPC and DMPC monolayers. However, the effect were much lower when the monolayer was composed by DMPE. The low hydration and strong head-head interaction between the phosphates and the amine groups of adjacent PE's would result in an area per molecule much lower in PE than in phosphatidylcholine (PC) in concordance with the lower penetration in PE. Protease adsorption on PE membranes increases the proteolytic activity in which condition is less susceptible to inhibition by pepstatin. However, PC's do not alter the enzyme activity being the action of inhibitor unaffected.
authors.affiliation: Laboratorio de Fisicoquímica de Membranas Lipídicas y Liposomas, Cátedra de Química General e Inorgánica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, 956 2 degrees Piso (1113), Capital Federal, Junin, Argentina.
authors.forename: M F
authors.initials: MF
authors.lastname: Martini
authors.forename: E A
authors.initials: EA
authors.lastname: Disalvo
mesh_terms: D000327:Adsorption; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D008563:Membrane Lipids / Q000737:chemistry*; D009089:Mucor / Q000201:enzymology; D010743:Phospholipids / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D008563:Membrane Lipids; D010743:Phospholipids; D016282:Aspartic Acid Endopeptidases