Epstein-Barr virus (EBV) nuclear antigen 1 colocalizes with cellular replication foci in the absence of EBV plasmids.
Epstein-Barr virus (EBV) EBNA-1 is the only EBV-encoded protein that is essential for the once-per-cell-cycle replication and maintenance of EBV plasmids in latently infected cells. EBNA-1 binds to the oriP region of latent EBV plasmids and cellular metaphase chromosomes. In the absence of oriP-containing plasmids, EBNA-1 was highly colocalized with cellular DNA replication foci that were identified by immunostaining S-phase cells for proliferating cell nuclear antigen and replication protein A (RP-A) in combination with DNA short pulse-labeling. For the association of EBNA-1 with the cellular replication focus areas, the EBNA-1 regions of amino acids (aa) 8 to 94 and/or aa 315 to 410, but not the RP-A-interacting carboxy-terminal region, were necessary. These results suggest a new aspect of latent virus-cell interactions.
Ito
Sayuri
S
Herpesvirus Laboratory, Department of Virology I, National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, Japan.
Yanagi
Kazuo
K
D000818:Animals; D001402:B-Lymphocytes; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000378:metabolism; D019309:Epstein-Barr Virus Nuclear Antigens / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D051716:Replication Protein A; D016196:S Phase
D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; D008164:Luminescent Proteins; D018809:Proliferating Cell Nuclear Antigen; C497167:RPA1 protein, human; D011993:Recombinant Fusion Proteins; D051716:Replication Protein A; D049452:Green Fluorescent Proteins; C417029:EBV-encoded nuclear antigen 1