Transfer of the alpha 5(IV) collagen chain gene to smooth muscle restores in vivo expression of the alpha 6(IV) collagen chain in a canine model of Alport syndrome.
X-linked Alport syndrome is a progressive renal disease caused by mutations in the COL4A5 gene, which encodes the alpha 5(IV) collagen chain. As an initial step toward gene therapy for Alport syndrome, we report on the expression of recombinant alpha 5(IV) collagen in vitro and in vivo. A full-length cDNA-encoding canine alpha 5(IV) collagen was cloned and expressed in vitro by transfection of HEK293 cells that synthesize the alpha1(IV) and alpha2(IV), but not the alpha 3(IV) to alpha 6(IV) collagen chains. By Northern blotting, an alpha 5(IV) mRNA transcript of 5.2 kb was expressed and the recombinant protein was detected by immunocytochemistry. The chain was secreted into the medium as a 190-kd monomer; no triple helical species were detected. Transfected cells synthesized an extracellular matrix containing the alpha1(IV) and alpha2(IV) chains but the recombinant alpha 5(IV) chain was not incorporated. These findings are consistent with the concept that the alpha 5(IV) chain requires one or more of the alpha 3(IV), alpha 4(IV), or alpha 6(IV) chains for triple helical assembly. In vivo studies were performed in dogs with X-linked Alport syndrome. An adenoviral vector containing the alpha 5(IV) transgene was injected into bladder smooth muscle that lacks both the alpha 5(IV) and alpha 6(IV) chains in these animals. At 5 weeks after injection, there was expression of both the alpha 5(IV) and alpha 6(IV) chains by smooth muscle cells at the injection site in a basement membrane distribution. Thus, this recombinant alpha 5(IV) chain is capable of restoring expression of a second alpha(IV) chain that requires the presence of the alpha 5(IV) chain for incorporation into collagen trimers. This vector will serve as a useful tool to further explore gene therapy for Alport syndrome.
Harvey
Scott J
SJ
Division of Pathology, Hospital for Sick Children, and the Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada.
Zheng
Keqin
K
Jefferson
Barbara
B
Moak
Peter
P
Sado
Yoshikazu
Y
Naito
Ichiro
I
Ninomiya
Yoshifumi
Y
Jacobs
Robert
R
Thorner
Paul S
PS
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D024141:Collagen Type IV / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004195:Disease Models, Animal; D004285:Dogs; D007150:Immunohistochemistry; D008297:Male; D009130:Muscle, Smooth / Q000378:metabolism*; D009394:Nephritis, Hereditary / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
D024141:Collagen Type IV; D018076:DNA, Complementary; D020033:Protein Isoforms; D012333:RNA, Messenger; D011994:Recombinant Proteins