[A study on screening effective immunization route of anticaries DNA vaccine pcDNA3-gtfB].
OBJECTIVE
Glucosyltransferase-B (GTF-B) of Streptococcus mutans has been implicated as a principal virulent factor in the development of dental caries. The objective was to use recombined plasmid pcDNA-gtfB expressing multiple antigen of glucosyltransferase-B as gene vaccine to immunize rats through different route, and to investigate the immunization effects of immunization routes.


METHODS
A total of 18 Wistar rats were divided into 3 groups, including the quadriceps injection group, the intransal irrigation group and the submandibular gland-targeted injection group. The serum IgG and salivary IgA were assayed by using ELISA after pcDNA3-gtfB immunization. The serum IgG and salivary IgA in different groups were compared using statistical one-way ANOVA.


RESULTS
Compared these 3 groups, the serum IgG in the quadriceps injection group was much higher than those of other two groups (P < 0.01), while the salivary IgA of the submandibular gland-targeted injection was much higher than those of other two groups (P < 0.01).


CONCLUSIONS
It is indicated pcDNA3-gtfB is good candidate for anticarious gene vaccine, and submandibular gland-targeted injection is an effective immunization route for stimulating salivary IgA.
Yang
Jinbo
J
West China College of Stomatology, Sichuan University.
Liu
Tianjia
T
Zhuang
Heng
H
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology; D003001:Cloning, Molecular; D003731:Dental Caries / Q000517:prevention & control*; D005964:Glucosyltransferases / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011995:Recombination, Genetic; D012463:Saliva / Q000276:immunology; D013295:Streptococcus mutans / Q000235:genetics / Q000276:immunology*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D005964:Glucosyltransferases