Nicotinamide adenine dinucleotide stimulates oligomerization, interaction with adenovirus E1A and an intrinsic dehydrogenase activity of CtBP.
The C-terminal region of adenovirus E1A interacts with the transcriptional corepressor, CtBP. The mechanism of transcriptional regulation by CtBP is not known. CtBP shares a significant homology with NAD(+)-dependent D2-hydroxy acid dehydrogenases. CtBP binds to NAD(+) and NADH. Both forms of the dinucleotide stimulate oligomerization of native CtBP and enhance complex formation with E1A. CtBP also has a slow dehydrogenase activity. Interaction of CtBP with E1A reduces the dehydrogenase activity. Our results raise the possibility that the oxidation/reduction reactions of CtBP may regulate transcription. Thus, CtBP is a unique transcriptional regulator with an enzymatic activity similar to metabolic dehydrogenases. The levels of intracellular nicotinamide adenine dinucleotide may modulate transcriptional activity of CtBP.
Balasubramanian
P
P
Institute for Molecular Virology, Saint Louis University Health Sciences Center, 3681 Park Avenue, MO 63110, USA.
Zhao
Ling Jun
LJ
Chinnadurai
G
G
D017500:Adenovirus E1A Proteins / Q000737:chemistry / Q000378:metabolism*; D000429:Alcohol Oxidoreductases; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D046911:Macromolecular Substances; D009243:NAD / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014714:Vertebrates
D017500:Adenovirus E1A Proteins; D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D010750:Phosphoproteins; D011994:Recombinant Proteins; D009243:NAD; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C096794:C-terminal binding protein