title: Granulocyte colony-stimulating factor for the treatment of biomaterial-associated staphylococcal infections in-vitro.
abstract: Staphylococcal infections are a common and severe complication after the implantation of a prosthesis. We developed an in-vitro model for biomaterial-associated infections and studied the effects of human recombinant granulocyte colony-stimulating factor (rhuG-CSF; filgrastime) on the eradication of bacteria from the surface of biomaterial. Latex beads (25 micro m) were incubated with 10(7) colony forming units of either a slime producing (DSM 3269) or non-slime producing strain (ATCC 14990) of Staphylococcus epidermidis. Infected particles were consecutively confronted with effector cells, derived from heparinized whole blood samples taken from healthy volunteers, after stimulation with rhuG-CSF (5,000 IU/ml, 10,000 IU/ml). Control blood specimens were not stimulated or conditioned with normal saline. The results indicate that stimulation with rhuG-CSF induced an increased rate of phagocytosis and lead to a more rapid reduction of adhering bacteria from the surface of the beads. Therefore, the in-vitro data suggest that patients with prosthesis infection may profit from an additional treatment with rhuG-CSF.
authors.affiliation: Department of Urology, University of Aachen, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Schlöbe
authors.forename: Norbert
authors.initials: N
authors.lastname: Schnitzler
authors.forename: Klaus
authors.initials: K
authors.lastname: Schweizer
authors.forename: Detlef
authors.initials: D
authors.lastname: Rohde
mesh_terms: D000328:Adult; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008863:Microspheres; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000276:immunology / Q000382:microbiology; D010587:Phagocytosis / Q000187:drug effects*; D011994:Recombinant Proteins / Q000494:pharmacology; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000276:immunology; D013212:Staphylococcus epidermidis
chemical_list: D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor