title: Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli.
abstract: The gene encoding a putative mature antitumor-analgesic peptide (AGAP) from the venom of the Chinese scorpion Buthus martensii Karsch was obtained by polymerase chain reaction (PCR) according to its cDNA sequence and expressed in Escherichia coli. While most of the recombinant AGAP was expressed in the form of insoluble inclusion body. The recombinant AGAP was purified to homogeneity by metal chelating affinity chromatography. Pharmaceutical tests showed that the recombinant AGAP has both analgesic and antitumor activities on mice.
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Liu
authors.forename: Run-Lin
authors.initials: RL
authors.lastname: Ma
authors.forename: Si-Ling
authors.initials: SL
authors.lastname: Wang
authors.forename: Zi-Yuan
authors.initials: ZY
authors.lastname: Duan
authors.forename: Jin-Hai
authors.initials: JH
authors.lastname: Zhang
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Wu
authors.forename: Chun-Fu
authors.initials: CF
authors.lastname: Wu
mesh_terms: D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002846:Chromatography, Affinity; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D005822:Genetic Vectors; D051379:Mice; D009368:Neoplasm Transplantation; D010455:Peptides / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012604:Scorpion Venoms / Q000737:chemistry* / Q000494:pharmacology; D012605:Scorpions; D014407:Tumor Cells, Cultured
chemical_list: D000700:Analgesics; D000970:Antineoplastic Agents; D018076:DNA, Complementary; D010455:Peptides; D011994:Recombinant Proteins; D012604:Scorpion Venoms; C477804:antitumor-analgesic peptide, Buthus martensii