title: Roles of ionotropic glutamate receptors in early developing neurons derived from the P19 mouse cell line.
abstract: We cultured a P19 mouse teratocarcinoma cell line and induced its neuronal differentiation to study the function of ionotropic glutamate receptors (GluRs) in early neuronal development. Immunocytochemical studies showed 85% neuronal population at 5 days in vitro (DIV) with microtubule-associated protein 2-positive staining. Thirty percent and 50% of the cells expressed the alpha-amino-3-hydroxy-5-methyl-4-isopropinonate (AMPA) receptor subunit, GluR2/3, and the kainate (kainic acid; KA) receptor subunit, GluR5/6/7, respectively. In Western blot analysis, the temporal expression of GluR2/3 began to appear at 3 DIV, whereas GluR5/6/7 was already expressed in the undifferentiated cells. P19-derived neurons began to respond to glutamate, AMPA and KA, but not to the metabotropic GluR agonist trans-1-aminocyclopentane-1,3-decarboxylic acid, by 5 DIV in terms of increases in intracellular calcium and phospholipase C-mediated poly-phosphoinositide turnover. Furthermore, KA reduced cell death of P19-derived neurons in both atmospheric and hypobaric conditions in a phospholipase C-dependent manner. The common AMPA/KA receptor antagonist, 6-cyano-7-nitroquinoxaline-2,3-dione, but not the AMPA receptor antagonist, 1,2,3,4-tetrahydro-6-nitro-2,3-dioxo-benzo[f]quinoxaline-7-sulfonamide disodium, profoundly increased hypobaric insult-induced neurotoxicity. In a flow cytometry study, the nerve growth factor-mediated antiapoptotic effect was facilitated by AMPA, with an induction of TrkA, but not p75(NTR) expression. Therefore, AMPA and KA receptors might mediate neurotrophic functions to facilitate neurotrophic factor signaling to protect neurons against hypoxic insult in early neuronal development.
authors.affiliation: Department of Physiology, Taipei Medical University, Taipei, Taiwan, ROC. hsuan@tmu.edu.tw
authors.forename: Yi-Hsuan
authors.initials: YH
authors.lastname: Lee
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Lin
authors.forename: Li-Wen
authors.initials: LW
authors.lastname: Hsu
authors.forename: Ssu-Yao
authors.initials: SY
authors.lastname: Hu
authors.forename: Wen-Te
authors.initials: WT
authors.lastname: Hsiao
authors.forename: Yuan-Soon
authors.initials: YS
authors.lastname: Ho
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation; D002460:Cell Line; D045744:Cell Line, Tumor; D003515:Cycloleucine / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D000860:Hypoxia; D007150:Immunohistochemistry; D007608:Kainic Acid / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008858:Microscopy, Phase-Contrast; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000737:chemistry; D020800:Receptor, Nerve Growth Factor; D020917:Receptor, trkA; D018091:Receptors, AMPA / Q000737:chemistry / Q000502:physiology*; D018092:Kainic Acid Receptors / Q000502:physiology; D017475:Receptors, Nerve Growth Factor / Q000737:chemistry; D013997:Time Factors; D000097806:GluK2 Kainate Receptor; D000097808:GluK3 Kainate Receptor
chemical_list: D002118:Calcium; D002352:Carrier Proteins; D003515:Cycloleucine; D007608:Kainic Acid; D008565:Membrane Proteins; D010716:Phosphatidylinositols; D020800:Receptor, Nerve Growth Factor; D020917:Receptor, trkA; D018091:Receptors, AMPA; D018092:Kainic Acid Receptors; D017475:Receptors, Nerve Growth Factor; D000097806:GluK2 Kainate Receptor; D000097808:GluK3 Kainate Receptor; C104723:glutamate receptor ionotropic, AMPA 3; C104722:glutamate receptor ionotropic, AMPA 2