title: Optimization of cationic lipid mediated gene transfer: structure-function, physico-chemical, and cellular studies.
abstract: The rationale design aimed at the enhancement of cationic lipid mediated gene transfer is discussed. These improvements are based on the straight evaluation of the structure-activity relationship and on the introduction of new structures. Much attention have been given to the supramolecular structures of the lipid/DNA complexes, to the effect of serum on gene transfer and to the intracellular trafficking of the lipoplexes. Finally new avenue using reducible cationic lipids has been discussed.
authors.affiliation: Laboratoire de Chimie Bioorganique et de Biotechnologie Moléculaire et Cellulaire, UMR 7001 CNRS ENSCP, Aventis ENSCP, Paris, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Carrière
authors.forename: Isabelle
authors.initials: I
authors.lastname: Tranchant
authors.forename: Pierre-Antoine
authors.initials: PA
authors.lastname: Niore
authors.forename: Gerardo
authors.initials: G
authors.lastname: Byk
authors.forename: Nathalie
authors.initials: N
authors.lastname: Mignet
authors.forename: Virginie
authors.initials: V
authors.lastname: Escriou
authors.forename: Daniel
authors.initials: D
authors.lastname: Scherman
authors.forename: Jean
authors.initials: J
authors.lastname: Herscovici
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002412:Cations; D004247:DNA / Q000378:metabolism; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D050356:Lipid Metabolism; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D008956:Models, Chemical; D011073:Polyamines / Q000737:chemistry; D013329:Structure-Activity Relationship; D014162:Transfection
chemical_list: D002412:Cations; D011073:Polyamines; D004247:DNA; D008156:Luciferases