title: Protective effects of hydrogen peroxide against ischemia/reperfusion injury in perfused rat hearts.
abstract: Among the several mechanisms proposed for ischemic preconditioning (IPC), generation of reactive oxygen species (ROS) is reported to be involved in the cardioprotective effects of IPC. The present study was designed to investigate whether repetitive exposure to hydrogen peroxide (H(2)O(2)) can protect the myocardium against subsequent ischemia/reperfusion injury, and whether the H(2)O(2)-induced cardioprotection is related to the preservation of energy metabolism. Langendorff-perfused rat hearts were exposed to two, 5 min episodes of IPC or to various concentrations of H(2)O(2) twice and then to 35 min global ischemia and 40 min reperfusion. Using (31)P nuclear magnetic resonance ((31)P-NMR) spectroscopy, cardiac phosphocreatine (PCr) and ATP and intracellular pH (pH(i)) were monitored. IPC and the treatment with 2 micromol/L H(2)O(2) significantly improved the post-ischemic recovery of left ventricular developed pressure (LVDP) and the PCr and ATP compared with those of the control ischemia/reperfusion (LVDP: 36.9 +/-7.4% of baseline in control hearts, 84.0+/-3.5% in IPC, 65.4+/-3.8% in H(2)O(2); PCr: 51.1+/-5.3% in control hearts, 81.4+/-5.5% in IPC, 81.7+/-5.2% in H(2)O(2); ATP: 12.3+/-1.6% in control hearts; 30.0+/-2.8% in IPC, 28.6+/-2.3% in H(2)O(2), mean +/- SE, p<0.05). However, lower (0.5 micromol/L) or higher (10 micromol/L) concentration of H(2)O (2) had no effect. There were significant linear correlations between mean LVDP and high-energy metabolites after 40 min reperfusion in H(2)O(2)-treated hearts. In IPC-treated hearts, the mean LVDP was greater than that in the 2 micromol/L H(2)O(2)-treated hearts under similar levels of high-energy metabolites. IPC also ameliorated intracellular acidification (6.38+/-0.03 in control hearts, 6.65+/-0.04 in IPC, p<0.05), but treatment with H(2)O(2) did not affect pH(i) during ischemia (6.40+/-0.05 in H(2)O(2)). In conclusion, H(2)O(2) had protective effects against ischemia/reperfusion injury and the effects were related to the preservation of energy metabolism. IPC could have additional protective mechanisms that are associated with the amelioration of intracellular acidosis during ischemia.
authors.affiliation: Division of Cardiology, Department of Internal Medicine III, Hamamatsu, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yaguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Satoh
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Wakahara
authors.forename: Hideki
authors.initials: H
authors.lastname: Katoh
authors.forename: Akihiko
authors.initials: A
authors.lastname: Uehara
authors.forename: Hajime
authors.initials: H
authors.lastname: Terada
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Fujise
authors.forename: Hideharu
authors.initials: H
authors.lastname: Hayashi
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D004734:Energy Metabolism; D006321:Heart / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D019194:Ischemic Preconditioning / Q000379:methods; D008297:Male; D019906:Nuclear Magnetic Resonance, Biomolecular; D010477:Perfusion; D010725:Phosphocreatine / Q000032:analysis; D010759:Phosphorus Isotopes; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy / Q000517:prevention & control*; D016277:Ventricular Function, Left
chemical_list: D010759:Phosphorus Isotopes; D020011:Protective Agents; D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D006861:Hydrogen Peroxide