The transcriptional activity of human Chromosome 22.
A DNA microarray representing nearly all of the unique sequences of human Chromosome 22 was constructed and used to measure global-transcriptional activity in placental poly(A)(+) RNA. We found that many of the known, related and predicted genes are expressed. More importantly, our study reveals twice as many transcribed bases as have been reported previously. Many of the newly discovered expressed fragments were verified by RNA blot analysis and a novel technique called differential hybridization mapping (DHM). Interestingly, a significant fraction of these novel fragments are expressed antisense to previously annotated introns. The coding potential of these novel expressed regions is supported by their sequence conservation in the mouse genome. This study has greatly increased our understanding of the biological information encoded on a human chromosome. To facilitate the dissemination of these results to the scientific community, we have developed a comprehensive Web resource to present the findings of this study and other features of human Chromosome 22 at http://array.mbb.yale.edu/chr22.
Rinn
John L
JL
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520-8103, USA.
Euskirchen
Ghia
G
Bertone
Paul
P
Martone
Rebecca
R
Luscombe
Nicholas M
NM
Hartman
Stephen
S
Harrison
Paul M
PM
Nelson
F Kenneth
FK
Miller
Perry
P
Gerstein
Mark
M
Weissman
Sherman
S
Snyder
Michael
M
D000595:Amino Acid Sequence; D000818:Animals; D002892:Chromosomes, Human, Pair 22; D017124:Conserved Sequence; D030541:Databases, Genetic; D005260:Female; D006801:Humans; D020407:Internet; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010920:Placenta / Q000502:physiology; D011061:Poly A; D014158:Transcription, Genetic
D011061:Poly A