Fate of orally administered 15N-labeled polyamines in rats bearing solid tumors.
We studied absorption, distribution, metabolism, and excretion of polyamines (putrescine, spermidine, and spermine) in the gastrointestinal tract using (15)N-labeled polyamines as tracers and ionspray ionization mass spectrometry (IS-MS). The relatively simple protocol using rats bearing solid tumors provided useful information. Three (15)N-labeled polyamines that were simultaneously administered were absorbed equally from gastrointestinal tract, and distributed within tissues at various concentrations. The uptake of (15)N-spermidine seemed preferential to that of (15)N-spermine since the concentrations of (15)N-spermidine in the liver and tumors were higher, whereas those of (15)N-spermine were higher in the kidney, probably due to the excretion of excess extracellular spermine. Most of the absorbed (15)N-putrescine seemed to be lost, suggesting blood and tissue diamine oxidase degradation. Concentrations of (15)N-spermidine and (15)N-spermine in the tumor were low. We also describe the findings from two rats that were administered with (15)N-spermine. The tissue concentrations of (15)N-spermine were unusually high, and significant levels of (15)N-spermidine were derived from (15)N-spermine in these animals.
Kobayashi
Masaki
M
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan.
Xu
Yong Ji
YJ
Samejima
Keijiro
K
Goda
Hitomi
H
Niitsu
Masaru
M
Takahashi
Masakazu
M
Hashimoto
Yoshiyuki
Y
D000284:Administration, Oral; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D004064:Digestive System / Q000187:drug effects / Q000378:metabolism; D005215:Fasting / Q000502:physiology; D007668:Kidney / Q000737:chemistry / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D009587:Nitrogen Isotopes / Q000032:analysis / Q000493:pharmacokinetics*; D011073:Polyamines / Q000032:analysis / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D009587:Nitrogen Isotopes; D011073:Polyamines