[Characterization of Rhizobium isolates from acid and alkaline soils in semi-arid regions of Pernambuco].
The genetic variety of the Rhizobium isolates from acid and alkaline soils in the semiarid zone of Pernambuco state was evaluated through the use of 17 primers of arbitrary sequence. Amplified products were separated by electrophoresis in agarose gel at 1.4% and visualized by ethidium bromide coloration. The results obtained suggest a high genetic variety of the isolates in relation to the standard strain. Data were analyzed by UPGMA (unweighted pair-group method with arithmetic average), based on Jaccard's coefficient and visualized through dendrograms. The strains isolated from the acid soils were included in one group whereas the strains from alkaline soils were located in other three groups. Meanwhile, one of the groups formed by strain Isol-14, isolated from acid soils is more related to the groups of strains isolated from acid soils than to the remaining groups from alkaline soils.
Da Silva
M L R Bastos
ML
Laboratório de Biologia de Solo, Empresa Pernambucana de Pesquisa Agropecuária (IPA), Av. General San Martins 1371, Bonji, Recife, Pernambuco, 50761-000. luiza@ipa.br
Burity
H Almeida
HA
Figueiredo
M V Barreto
MV
De Freitas
N S Aguiar
NS
Mergulhão
A C E S
AC
De Lyra
M C C Pereira
MC
D001482:Base Composition; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics; D006813:Humidity; D006863:Hydrogen-Ion Concentration; D019105:Random Amplified Polymorphic DNA Technique; D012231:Rhizobium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D013045:Species Specificity
D004269:DNA, Bacterial; D012987:Soil