Possible quorum sensing in the rumen microbial community: detection of quorum-sensing signal molecules from rumen bacteria.
The bioluminescence assay using Vibrio harveyi BB170 was used to examine quorum-sensing autoinducer 2 (AI-2) activity from cell-free culture fluids of rumen bacteria. The assay showed that the culture fluids of four species of rumen bacteria, Butyrivibrio fibrisolvens, Eubacterium ruminantium, Ruminococcus flavefaciens, and Succinimonas amylolytica, contained AI-2-like molecules. Furthermore, homologues for luxS genes were detected in rumen fluids collected from three cows and in bacterial cells of P. ruminicola subsp. ruminicola and R. flavefaciens. These findings suggest that the quorum-sensing system mediated by AI-2 is present in the rumen.
Mitsumori
Makoto
M
National Institute of Livestock and Grassland Science, Ikenodai 2, Tsukuba, 305-0901, Ibaraki, Japan. mitumori@affrc.go.jp
Xu
Liming
L
Kajikawa
Hiroshi
H
Kurihara
Mitsunori
M
Tajima
Kiyoshi
K
Hai
Jin
J
Takenaka
Akio
A
D000818:Animals; D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D013437:Carbon-Sulfur Lyases; D002417:Cattle; D017077:Culture Media, Conditioned; D015964:Gene Expression Regulation, Bacterial; D006714:Homoserine / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism*; D007783:Lactones / Q000378:metabolism*; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D010802:Phylogeny; D012417:Rumen / Q000382:microbiology; D017422:Sequence Analysis, DNA; D015398:Signal Transduction
D001426:Bacterial Proteins; D017077:Culture Media, Conditioned; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D006714:Homoserine; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria