Novel sample preparation method for safe and rapid detection of Bacillus anthracis spores in environmental powders and nasal swabs.
Bacillus anthracis spores have been used as a biological weapon in the United States. We wanted to develop a safe, rapid method of sample preparation that provided safe DNA for the detection of spores in environmental and clinical specimens. Our method reproducibly detects B. anthracis in samples containing <10 spores.
Luna
Vicki A
VA
Center for Biological Defense, Department of Environmental and Occupational Health, College of Public Health, University of South Florida, Tampa, Florida 33612, USA. vluna@bt.suf.edu
King
Debra
D
Davis
Carisa
C
Rycerz
Tony
T
Ewert
Matthew
M
Cannons
Andrew
A
Amuso
Philip
P
Cattani
Jacqueline
J
D001408:Bacillus anthracis / Q000302:isolation & purification*; D023482:Bioterrorism; D006801:Humans; D009666:Nose / Q000382:microbiology*; D011208:Powders; D013171:Spores, Bacterial / Q000302:isolation & purification*
D011208:Powders