title: Protective effect of a naked DNA vaccine cocktail against lethal toxoplasmosis in mice.
abstract: BALB/c mice were intramuscularly immunized with low doses (25-50microg) of DNA cocktail containing plasmids encoding the full-length SAG1/P30 and the 196-561 terminal sequence of ROP2 genes. This immunization resulted in a Th1-type response with predominance of IgG2a and a specific T-cell proliferation with high levels of interferon-gamma (IFN-gamma) secretion, whereas no IL-4 was detected. Moreover, DNA cocktail immunization induced a long-lasting protection against a lethal challenge with the highly virulent Toxoplasma gondii RH strain, whereas low doses of single genes were not protective. These results support further investigations to achieve a multigene anti-T. gondii DNA vaccine.
authors.affiliation: Laboratory of Autoimmunity and Immunoregulation, Department of Immunology, Oswaldo Cruz Institute, Fiocruz. Av Brasil, 4365, Rio de Janeiro 21045-900, Brazil.
authors.forename: Alberto
authors.initials: A
authors.lastname: Fachado
authors.forename: Alexandro
authors.initials: A
authors.lastname: Rodriguez
authors.forename: Sergio O
authors.initials: SO
authors.lastname: Angel
authors.forename: Diamar C
authors.initials: DC
authors.lastname: Pinto
authors.forename: Iviane
authors.initials: I
authors.lastname: Vila
authors.forename: Armando
authors.initials: A
authors.lastname: Acosta
authors.forename: Regina R
authors.initials: RR
authors.lastname: Amendoeira
authors.forename: Joseli
authors.initials: J
authors.lastname: Lannes-Vieira
mesh_terms: D000818:Animals; D007371:Interferon-gamma / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016052:Protozoan Vaccines / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000517:prevention & control*; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D016052:Protozoan Vaccines; D019444:Vaccines, DNA; D007371:Interferon-gamma