HPLC method for the analysis of harmol, harmalol, harmine and harmaline in the seeds of Peganum harmala L.
A simple and sensitive method for separation and determination of harmol, harmalol, harmine and harmaline has been developed and validated. Harmol, harmalol, harmine and harmaline were separated using a Metasil ODS column by isocratic elution with flow rate 1.5 ml/min. The mobile phase composition was Isopropyl alcohol-Acetonitrile-Water-Formic acid (100:100:300:0.3) (v/v/v/v) and pH adjusted 8.6 with triethylamine. Spectrophotometric detection was carried out at 330 nm. The linear range of detection for harmol, harmalol, harmine and harmaline were between 9.375-250, 30.750-246, 31.250-500 and 31.000-248 microg/ml, respectively. The method described was suitable for the determination of harmol, harmalol, harmine and harmaline in the seeds of Peganum harmala L.
Kartal
M
M
Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey.
Altun
M L
ML
Kurucu
S
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006246:Harmaline / Q000031:analogs & derivatives* / Q000032:analysis*; D006247:Harmine / Q000031:analogs & derivatives* / Q000032:analysis*; D032443:Peganum / Q000737:chemistry / Q000196:embryology*; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
C007904:harmalol; C001326:harmol; D006247:Harmine; D006246:Harmaline