Comparison of ribotyping and repetitive extragenic palindromic-PCR for identification of fecal Escherichia coli from humans and animals.
This report compares the performances of two popular genotypic methods used for tracking the sources of fecal pollution in water, ribotyping and repetitive extragenic palindromic-PCR (rep-PCR). The rep-PCR was more accurate, reproducible, and efficient in associating DNA fingerprints of fecal Escherichia coli with human and animal hosts of origin.
Carson
C Andrew
CA
Department of Veterinary Pathobiology, 201 Connaway Hall, University of Missouri, Columbia, MO 65211, USA. carsonc@missouri.edu
Shear
Brian L
BL
Ellersieck
Mark R
MR
Schnell
Jennifer D
JD
D000818:Animals; D002417:Cattle; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D004285:Dogs; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics; D005243:Feces / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D021521:Ribotyping
D004269:DNA, Bacterial