title: Sex-specific and blood meal-induced proteins of Anopheles gambiae midguts: analysis by two-dimensional gel electrophoresis.
abstract: BACKGROUND
  Anopheles gambiae is the main vector of Plasmodium falciparum in Africa. The mosquito midgut constitutes a barrier that the parasite must cross if it is to develop and be transmitted. Despite the central role of the mosquito midgut in the host/parasite interaction, little is known about its protein composition. Characterisation of An. gambiae midgut proteins may identify the proteins that render An. gambiae receptive to the malaria parasite.
  
  
  METHODS
  We carried out two-dimensional gel electrophoresis of An. gambiae midgut proteins and compared protein profiles for midguts from males, sugar-fed females and females fed on human blood.
  
  
  RESULTS
  Very few differences were detected between male and female mosquitoes for the approximately 375 silver-stained proteins. Male midguts contained ten proteins not detected in sugar-fed or blood-fed females, which are therefore probably involved in male-specific functions; conversely, female midguts contained twenty-three proteins absent from male midguts. Eight of these proteins were specific to sugar-fed females, and another ten, to blood-fed females.
  
  
  CONCLUSIONS
  Mass spectrometry analysis of the proteins found only in blood-fed female midguts, together with data from the recent sequencing of the An. gambiae genome, should make it possible to determine the role of these proteins in blood digestion or parasite receptivity.
authors.affiliation: Ecologie des Systèmes Vectoriels, Institut Pasteur, 25 Rue du Dr Roux, 75724 Paris Cedex15, France. ghislaine.prevot-linguet@guyane.univ-ag.fr
authors.forename: G I
authors.initials: GI
authors.lastname: Prévot
authors.forename: C
authors.initials: C
authors.lastname: Laurent-Winter
authors.forename: F
authors.initials: F
authors.lastname: Rodhain
authors.forename: C
authors.initials: C
authors.lastname: Bourgouin
mesh_terms: D000818:Animals; D000852:Anopheles / Q000737:chemistry / Q000378:metabolism* / Q000469:parasitology; D004063:Digestion; D004064:Digestive System / Q000737:chemistry / Q000378:metabolism / Q000469:parasitology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D010963:Plasmodium falciparum / Q000378:metabolism; D011817:Rabbits; D012727:Sex Characteristics
chemical_list: D019476:Insect Proteins