Physicochemical properties of amphotericin B liposomes prepared by reverse-phase evaporation method.
The physicochemical properties of phosphatidylcholine-cholesterol liposomes containing amphotericin B and prepared by reverse-phase evaporation method were studied. Uniformly dispersed liposomal suspensions were obtained by employing 3:1 ratio (by volume) of diethyl ether to normal saline, 5 min sonication time at 7 degrees C, and evaporation of diethyl ether at 25 degrees C. Microscopic examination showed that the prepared liposomes were spheroids with unilamellar, oligolamellar, or multilamellar structure. The liposomes containing amphotericin B 2.0 mol% of total lipid led to the highest percentage of drug entrapment. Liposomes with maximum entrapment efficiency were obtained from using 250 micromol of total lipid. The liposomal amphotericin B possessing the highest drug entrapment efficiency (approximately 95%) with particle size range of 1307-1451 nm was the one composed of 1:1 molar ratio of phosphatidylcholine to cholesterol.
Rojanapanthu
Pleumchitt
P
Department of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Sarisuta
Narong
N
Chaturon
Korakot
K
Kraisintu
Krisana
K
D000666:Amphotericin B / Q000737:chemistry*; D000935:Antifungal Agents / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002784:Cholesterol / Q000737:chemistry; D004339:Drug Compounding; D008081:Liposomes; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D010713:Phosphatidylcholines / Q000737:chemistry; D014867:Water / Q000737:chemistry
D000935:Antifungal Agents; D008081:Liposomes; D010713:Phosphatidylcholines; D014867:Water; D000666:Amphotericin B; D002784:Cholesterol