Biochemical enzyme activity in different tissues of rats exposed to a novel phosphorothionate (RPR-V).
The effects of a novel phosphorothionate (RPR-V) synthesized at Indian Institute of Chemical Technology, Hyderabad, was studied using three sub-chronic doses of 0.033 (low), 0.066 (medium) and 0.099 (high) mg kg(-1) in male and female rats for a period of 90 days. This long term and repeated treatment of RPR-V revealed that the membrane bound target enzymes Aspartate aminotransferase and Alanine aminotransferase increased significantly in serum and kidney, whereas these enzymes significantly decreased in liver and lung tissues when measured after 45 and 90 days of treatment. This compound also caused significant inhibition of RBC Acetylcholinesterase, target enzyme of organophosphorus compounds revealing its effect on normal synaptic transmission. Two way Anova studies disclosed that the alterations were mostly dose and time dependent, sexual dimorphism was not observed when the activities of male rats were compared with female rats. Enzyme recoveries were recorded after 28 days of post treatment, high degree positive correlation was observed with regard to these enzymes between serum versus kidney, whereas in case of serum versus liver and lung tissues high degree negative correlation was recorded. These enzyme profiles elucidates that they increased in serum but they decreased significantly in liver and lung indicating necrosis of these tissues. However, in case of kidney the level of these enzymes increased significantly with parallel to serum, which is suggestive of an increase synthesis of these enzymes, may be an adaptive mechanism due to the stress of the toxicant. These biomarker enzymes can be detected rapidly and hence may be used for the prediction and diagnosis of pesticide insults.
Rahman
Mohammed F
MF
Biochemical Toxicology, Biology Division, Indian Institute of Chemical Technology, Hyderabad, India. rahman@iict.ap.nic.in
Siddiqui
Mohammed K J
MK
D000110:Acetylcholinesterase / Q000097:blood / Q000378:metabolism; D000410:Alanine Transaminase / Q000097:blood / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood / Q000378:metabolism; D015415:Biomarkers; D004305:Dose-Response Relationship, Drug; D005260:Female; D007306:Insecticides / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008168:Lung / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008999:Monocrotophos / Q000031:analogs & derivatives* / Q000633:toxicity*; D009946:Organothiophosphorus Compounds; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D013438:Sulfhydryl Compounds / Q000633:toxicity*
D015415:Biomarkers; D007306:Insecticides; D009946:Organothiophosphorus Compounds; D013438:Sulfhydryl Compounds; C066790:RPR 5; D008999:Monocrotophos; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000110:Acetylcholinesterase