The spleen modulates the febrile response of guinea pigs to LPS.
The febrile responses of splenectomized (Splex) or sham-operated (Sham) guinea pigs challenged intravenously or intraperitoneally with lipopolysaccharide (LPS) 7 and 30 days after surgery were evaluated. FITC-LPS uptake by Kupffer cells (KC) was additionally assessed 15, 30, and 60 min after injection. LPS at 0.05 microg/kg iv did not evoke fever in Sham animals but caused a 1.2 degrees C core temperature (T(c)) rise in the Splex animals. LPS at 2 microg/kg iv induced a 1.8 degrees C greater T(c) rise of the Splex animals than of their controls. LPS at 2 and 8 microg/kg ip 7 days postsurgery induced 1.4 and 1.8 degrees C higher fevers, respectively, in the Splex than Sham animals. LPS at 2 and 8 microg/kg ip 30 days postsurgery also increased the febrile responses of the asplenic animals by 1.6 and 1.8 degrees C, respectively. FITC-LPS at 7 days was detected in the controls within KC 15 min after its administration; the label density was reduced at 30 min and almost 0 at 60 min. In the Splex group, in contrast, the labeling was significantly denser and remained unchanged through all three time points; this effect was still present 30 days after surgery. Similar results were obtained at 60 min after FITC-LPS intraperitoneal injection. Gadolinium chloride pretreatment (-3 days) of the Splex group significantly reduced both their febrile responses to LPS (8 microg/kg ip) and their KC uptake of FITC-LPS 7 days postsurgery. Thus splenectomy increases the magnitude of the febrile response of guinea pigs and the uptake of systemically administered LPS.
Feleder
Carlos
C
Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA.
Li
Zhonghua
Z
Perlik
Vit
V
Evans
Allison
A
Blatteis
Clark M
CM
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001831:Body Temperature / Q000187:drug effects; D005334:Fever / Q000139:chemically induced* / Q000503:physiopathology*; D016650:Fluorescein-5-isothiocyanate; D005682:Gadolinium / Q000494:pharmacology; D006168:Guinea Pigs; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D007728:Kupffer Cells / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D013154:Spleen / Q000187:drug effects* / Q000502:physiology*; D013156:Splenectomy; D013997:Time Factors
D008070:Lipopolysaccharides; D005682:Gadolinium; D016650:Fluorescein-5-isothiocyanate; C038958:gadolinium chloride