[Combined effect of lead and cadmium on lipid peroxidation in renal tubular epithelial cells of rats].
In order to observe the combined effects of lead and cadmium on lipid peroxidation, the cultured renal tubular epithelial cells (TEC) of rats are used to perform 3 x 3 factorial experimental design. In the experimental group (A-H), rats are given lead and cadmium respectively for 6 hours at different concentrations (A: lead acetate 0.02 mmol/L; B: lead acetate 0.1 mmol/L; C: cadmium chloride 0.001 mmol/L; D: cadmium chloride 0.004 mmol/L; E: lead acetate 0.02 mmol/L + cadmium chloride 0.001 mmol/L; F: lead acetate 0.02 mmol/L + cadmium chloride 0.004 mmol/L; G: lead acetate 0.1 mmol/L + cadmium chloride 0.001 mmol/L; H: lead acetate 0.1 mmol/L + cadmium chloride 0.004 mmol/L). After TEC are smashed by supersonic, the concentrations of glutathione (GSH) and malondiadehyde (MDA) in cells are analyzed. The same analysis is also done for the determination of the activity of glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD). In comparison with control group, significant decrease in activities of GSH, GSH-Px, SOD in cells treated with lead and cadmium and increases of MDA are observed. The data from ANOVA analysis show that the interaction between lead and cadmium on GSH, MDA, and SOD in TEC (P < 0.05) is demonstrated while no interaction on GSH-Px is shown.
Wang
Xueqian
X
Institute of Environmental Health Monitoring, Chinese Academy of Preventive Medicine, Beijing 100021, China.
Yin
Xianren
X
Bai
Xuetao
X
D000818:Animals; D019256:Cadmium Chloride / Q000633:toxicity*; D002478:Cells, Cultured; D004347:Drug Interactions; D004847:Epithelial Cells / Q000166:cytology; D005978:Glutathione / Q000378:metabolism; D007684:Kidney Tubules / Q000166:cytology* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009942:Organometallic Compounds / Q000633:toxicity*; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
D009942:Organometallic Compounds; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione; D019256:Cadmium Chloride; C008261:lead acetate