Cr(VI) reduction by sulfidogenic and nonsulfidogenic microbial consortia.
In time course experiments, bacterial community compositions were compared between a sulfidogenic and two nonsulfidogenic Cr(VI)-reducing consortia enriched from metal-contaminated sediments. The consortia were subjected to 0 and 0.85 mM or 1.35 mM Cr(VI), and Cr(VI) reduction, growth, and denaturing gradient gel electrophoresis profiles of PCR products of small-subunit (16S) ribosomal genes were compared. Results showed that although Cr(VI) was completely reduced by the three consortia, Cr(VI) inhibited cell growth, with sulfate-reducing bacteria being particularly sensitive to Cr(VI) toxicity relative to other bacteria in the consortia.
Arias
Y Meriah
YM
Marine Biology Research Division and Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California-San Diego, La Jolla, CA 92093-0202, USA.
Tebo
Bradley M
BM
D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D002857:Chromium / Q000378:metabolism*; D003470:Culture Media; D017753:Ecosystem; D004586:Electrophoresis / Q000379:methods; D019015:Geologic Sediments / Q000382:microbiology; D010084:Oxidation-Reduction; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012623:Seawater; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D016999:Sulfur-Reducing Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D014877:Water Pollution, Chemical
D003470:Culture Media; D002857:Chromium; C074702:chromium hexavalent ion