Contrast media augmented apoptosis of cultured renal mesangial, tubular, epithelial, endothelial, and hepatic cells.
OBJECTIVE
Nephrotoxicity of contrast media, resulting in apoptosis and acute necrosis of tubular cells, is well documented. No studies concerning mesangial cells apoptosis have been published yet.


OBJECTIVE
Apoptosis of cultured mesangial, tubular, and hepatic cell lines was investigated following exposure to different contrast media.


METHODS
Apoptosis was assessed by TUNEL assay and verified by Mayer Hematoxylin staining.


RESULTS
Iopromide, Ioxaglate, and Ioxatalamate induced apoptosis in all cell cultures at final concentrations ranged from 0.1% to 10.0%. However, only 1% to 10% Iomeprol elicited a significant apoptosis. Moreover, at 10% concentration, Iomeprol induced significantly less apoptosis than Iopromide, Ioxaglate, or Ioxatalamate.


CONCLUSIONS
First, Iomeprol, which has a different physico-chemical properties, proved to be less proapoptotic compared with other contrast compounds. Second, all types of cells similarly respond by apoptosis to contrast media induced injury. However, apoptosis of mesangial cells might generate additional deleterious effects in vivo.
Peer
Amir
A
Nephrology Division and the Department of Interventional Radiology, Assaf Harofeh Medical Center, Zerifin, Israel.
Averbukh
Zhan
Z
Berman
Sylvia
S
Modai
David
D
Averbukh
Michael
M
Weissgarten
Joshua
J
D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D003287:Contrast Media / Q000633:toxicity*; D004730:Endothelium, Vascular / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects; D005920:Glomerular Mesangium / Q000187:drug effects*; D007684:Kidney Tubules / Q000166:cytology / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D014283:Triiodobenzoic Acids / Q000633:toxicity
D003287:Contrast Media; D014283:Triiodobenzoic Acids