Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
Additional targets of CodY, a GTP-activated repressor of early stationary-phase genes in Bacillus subtilis, were identified by combining chromatin immunoprecipitation, DNA microarray hybridization, and gel mobility shift assays. The direct targets of CodY newly identified by this approach included regulatory genes for sporulation, genes that are likely to encode transporters for amino acids and sugars, and the genes for biosynthesis of branched-chain amino acids.
Molle
Virginie
V
Department of Cellular and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Nakaura
Yoshiko
Y
Shivers
Robert P
RP
Yamaguchi
Hirotake
H
Losick
Richard
R
Fujita
Yasutaro
Y
Sonenshein
Abraham L
AL
D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins; D015964:Gene Expression Regulation, Bacterial; D007763:Lac Operon / Q000235:genetics; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011233:Precipitin Tests / Q000379:methods*; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D002843:Chromatin; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins