Screening and characterization of human single-chain Fv antibody against beta-amyloid peptide 40.
Expression of single-chain variable fragment antibody on the surface of filamentous bacteriophage is widely used to make antibodies with pre-defined specificities. Using direct selection on solid phase-bound amyloid-beta 40 peptide, which is a main pathogenic feature of Alzheimer's disease, we obtained single-chain Fv antibody with specific binding activity. The binding epitope of the antibody is located between amino acids 1 and 16 of the peptide antigen. DNA sequencing showed that the gene coding for the single chain Fv antibody consists of 768 bp, and the complementarity-determining regions were deduced.
Cai
Jiong
J
Department of Nuclear Medicine, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Science, Beijing, China.
Wang
Shizhen
S
Zhong
Yanwei
Y
Cheng
Jun
J
Ji
Zhijuan
Z
Sheng
Shuli
S
Zhang
Chun
C
D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D017104:Bacteriophage M13 / Q000235:genetics; D015723:Gene Library; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
D016229:Amyloid beta-Peptides; D007135:Immunoglobulin Variable Region; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40)