Expression of isotocin-neurophysin mRNA in developing zebrafish.
Neurohypophysial peptides are important regulators of homeostasis, reproduction and behavior. We have sequenced a zebrafish cDNA representing isotocin-neurophysin (IT-NP) mRNA. The developmental expression pattern of zebrafish IT-NP mRNA was determined by whole-mount in situ hybridization histochemistry. At 32 h post fertilization (hpf) no IT-NP mRNA is detected. However, by 36 hpf, staining for IT-NP mRNA is detected in a tight bilateral cluster of cells located in the anterior hypothalamus. The IT-NP mRNA expression pattern remains remarkably stable throughout further development at least until 120 hpf.
Unger
Jennifer L
JL
Department of Neurobiology and Pharmacology, Northeastern Ohio Universities College of Medicine, Rootstown, OH 44272, USA.
Glasgow
Eric
E
D000595:Amino Acid Sequence; D000818:Animals; D008969:Molecular Sequence Data; D009481:Neurophysins / Q000096:biosynthesis / Q000235:genetics*; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics*; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology*
D009481:Neurophysins; D012333:RNA, Messenger; D010121:Oxytocin; C008215:isotocin