A human peripheral blood monocyte-derived subset acts as pluripotent stem cells.
We have identified, cultured, characterized, and propagated adult pluripotent stem cells (PSC) from a subset of human peripheral blood monocytes. These cells, which in appearance resemble fibroblasts, expand in the presence of macrophage colony-stimulating factor and display monocytic and hematopoietic stem cell markers including CD14, CD34, and CD45. We have induced these cells to differentiate into mature macrophages by lipopolysaccharide, T lymphocytes by IL-2, epithelial cells by epidermal growth factor, endothelial cells by vascular endothelial cell growth factor, neuronal cells by nerve growth factor, and liver cells by hepatocyte growth factor. The pluripotent nature of individual PSC was further confirmed by a clonal analysis. The ability to store, expand, and differentiate these PSC from autologous peripheral blood should make them valuable candidates for transplantation therapy.
Zhao
Yong
Y
Biochip Technology Center, Argonne National Laboratory, Argonne, IL 60439, USA.
Glesne
David
D
Huberman
Eliezer
E
D018952:Antigens, CD34 / Q000096:biosynthesis; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D004847:Epithelial Cells / Q000166:cytology; D022781:Hepatocytes / Q000166:cytology; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D050356:Lipid Metabolism; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D009000:Monocytes / Q000166:cytology*; D009474:Neurons / Q000166:cytology; D010587:Phagocytosis; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology*; D013601:T-Lymphocytes / Q000166:cytology; D013997:Time Factors
D018952:Antigens, CD34; D007376:Interleukin-2; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D017493:Leukocyte Common Antigens