Slit and robo: expression patterns in lung development.
First described as an axonal guidance cue through its repulsive effect on neurons expressing its receptor Roundabout (Robo), the Slit ligand has effects on cell migration, axon branching and elongation. Indirect evidence implicates Slit and Robo in lung development. We now demonstrate that Slit-2 and Slit-3 are developmentally regulated in embryonic murine lung. Immunohistochemistry demonstrates Slit-2 and Slit-3 expression by the pulmonary mesenchyme and airway epithelium. Robo-1 and Robo-2 are also expressed by the developing mesenchyme and airway epithelium. As lung development progresses, Robo-1 and Robo-2 expression localizes to only the airway epithelium. We conclude Slit/Robo are expressed in temporo-spatially adjacent domains suggesting interactive roles in pulmonary bronchiolar development.
Anselmo
Mark A
MA
Pediatric Pulmonary Unit, Massachusetts General Hospital for Children, Harvard Medical School, Jackson 14, GRJ 1416, 55 Fruit St, Boston, MA 02114, USA.
Dalvin
Sussie
S
Prodhan
Parthak
P
Komatsuzaki
Katsumi
K
Aidlen
Jeremy T
JT
Schnitzer
Jay J
JJ
Wu
Jane Y
JY
Kinane
T Bernard
TB
D000818:Animals; D029721:Drosophila Proteins; D020869:Gene Expression Profiling; D008168:Lung / Q000196:embryology* / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics*; D000097905:Roundabout Proteins
D029721:Drosophila Proteins; D009419:Nerve Tissue Proteins; D011971:Receptors, Immunologic; D000097905:Roundabout Proteins; C066819:sli protein, Drosophila