[Construction of recombinant plasmids containing genes of HBsAg and their expression in the eukaryotic cells].
OBJECTIVE
To construct recombinant plasmids expressing L, M, S and pre-S1-S protein of HBsAg.


METHODS
Amplifying segments of S, pre-S2-S, pre-S1-pre-S2-S genes of HBV by PCR and amplifying segment of pre-S1-S by overlap extension PCR; inserting the segments into Rc/CMV and pSG5UTPL/Flag plasmids respectively and exploring their expressions by Western-Blot hybridization, identifying the inserting segments by sequencing.


RESULTS
The sequences of the inserted segments were the same as the genes of S, pre-S2-S, pre-S1-pre-S2-S and pre-S1-S and the results of Western-Blot hybridization were positive for the aimed proteins.


CONCLUSIONS
We have gained 8 recombinant plasmids expressing S, M, L and pre-S1-S proteins with high efficacy.
Qin
S
S
Viral Hepatitis Research Unit, First Affiliated Hospital, WCUMS, Chengdu 610041, China.
Tang
H
H
Zhao
L
L
Liu
L
L
Lin
Y
Y
D001483:Base Sequence; D003001:Cloning, Molecular; D005057:Eukaryotic Cells / Q000378:metabolism*; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis / Q000235:genetics*; D017325:Hepatitis B Vaccines / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics*; D019444:Vaccines, DNA / Q000096:biosynthesis / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics
D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D011498:Protein Precursors; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; C050806:presurface protein 1, hepatitis B surface antigen