Fibroblastic, hematopoietic, and hormone responsive epithelial cell lines and culture conditions for elucidation of signal transduction and drug resistance pathways by gene transfer.
Elucidation of signal transduction pathways involved in proliferation, cell cycle progression and the regulation of apoptosis has shown great promise in the treatment of various diseases including neoplastic, inflammatory, autoimmune, immunodeficiency, arthritic and neurodegenerative disorders. By understanding how these signal transduction pathways function, chemotherapeutic targets may be identified which will suppress or eliminate the disease. This information may eventually be translated into therapy, which would either eliminate or safely contain the patient's disease. This chapter will focus on basic tissue culture techniques which are used to elucidate signal transduction pathways. Furthermore, this chapter will provide a general background for understanding how gene transfer techniques can be used to elucidate signal transduction pathways as well as various pitfalls commonly encountered with their usage.
Steelman
Linda S
LS
Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC, USA.
Blalock
William L
WL
Wang
Xiao-Yang
XY
Moye
Phillip W
PW
Lee
John T
JT
Shelton
John G
JG
Navolanic
Patrick M
PM
Davis
Julianne M
JM
Knapp
Steven L
SL
Franklin
Richard A
RA
White
Martyn K
MK
McCubrey
James A
JA
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D018014:Gene Transfer Techniques; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology; D006801:Humans; D015398:Signal Transduction / Q000502:physiology*