title: Single nucleotide polymorphisms in the 3' untranslated region of vascular endothelial growth factor gene in Japanese population with or without renal cell carcinoma.
abstract: We investigated native Japanese subjects whether C702T, C936T and G1612A polymorphisms in the 3' untranslated region (3'-UTR) of vascular endothelial growth factor (VEGF) gene are associated with the risk of renal cell carcinoma (RCC). Genomic DNAs from 145 RCC patients and 145 healthy controls were examined by polymerase chain reaction-based restriction fragment length polymorphism. Variant allele frequencies of C702T, C936T and G1612A were 0.00, 0.20 and 0.13 in the controls, respectively. The C702T and G1612A allele frequencies were significantly different between the Japanese population and the Caucasian population reported elsewhere. For each of C936T and G1612A polymorphisms, there was no statistically significant difference in the distribution of genotype frequencies between the cases and controls. Odds ratios and 95% confidence intervals computed by logistic regression analyses were not statistically significant. Stratification for the RCC cases according to pathological cell subtype, grade or stage failed to reveal any significant heterogeneity with respect to the genotype of each VEGF polymorphism. We revealed that there are significant ethnic differences in the C702T and G1612A allele frequencies, but suggested that C702T, C936T and G1612A polymorphisms in the 3'-UTR of VEGF gene are not associated with the risk of RCC, at least in Japanese population.
authors.affiliation: Department of Urology, Akita University School of Medicine, Akita, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Abe
authors.forename: Kazunari
authors.initials: K
authors.lastname: Sato
authors.forename: Tomonori
authors.initials: T
authors.lastname: Habuchi
authors.forename: Lizhong
authors.initials: L
authors.lastname: Wang
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Li
authors.forename: Norihiko
authors.initials: N
authors.lastname: Tsuchiya
authors.forename: Chikara
authors.initials: C
authors.lastname: Ohyama
authors.forename: Shigeru
authors.initials: S
authors.lastname: Satoh
authors.forename: Osamu
authors.initials: O
authors.lastname: Ogawa
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Kato
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D007564:Japan; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide
chemical_list: D020413:3' Untranslated Regions