title: Efficient release of overproduced gene products from Escherichia coli BL21(DE3) by lytic infection with newly isolated bacteriophages.
abstract: Overproduced proteins from Escherichia coli BL21(DE3) were efficiently released with virulent bacteriophages. Leviviridae-like bacteriophages were isolated from soil and used to lyse BL21(DE3) cells transformed with beta-glucosidase, chitinase, or chitosanase genes. This method caused lysis of bacterial cells similar to that by conventional sonication and enabled us to effectively recover and purify the enzymes.
authors.affiliation: Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University, Nara 631-8505, Japan.
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Iida
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Matsuda
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Saito
authors.forename: Masanori
authors.initials: M
authors.lastname: Nakasato
authors.forename: Teruo
authors.initials: T
authors.lastname: Nonomura
authors.forename: Koji
authors.initials: K
authors.lastname: Kakutani
authors.forename: Yukio
authors.initials: Y
authors.lastname: Tosa
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Mayama
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Toyoda
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D002688:Chitinases / Q000096:biosynthesis / Q000302:isolation & purification; D003851:Deoxyribonucleases / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000302:isolation & purification; D017907:Leviviridae / Q000737:chemistry*; D008854:Microscopy, Electron; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000302:isolation & purification; D012260:Ribonucleases / Q000737:chemistry; D012988:Soil Microbiology; D014465:Ultrasonics; D001617:beta-Glucosidase / Q000096:biosynthesis / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D003851:Deoxyribonucleases; D012260:Ribonucleases; D006026:Glycoside Hydrolases; C019868:chitosanase; D002688:Chitinases; D001617:beta-Glucosidase