[Primitive study of the cell lineage and Epstein-Barr virus infection in so called malignant histiocytosis with tissue microarray technique].
OBJECTIVE
To investigate the cell lineage and Epstein-Barr virus infection in previously diagnosed cases of malignant histiocytosis (MH) with tissue microarray technique.


METHODS
Using tissue-chips, immunohistochemical staining, in situ hybridization and PCR to analyze 5 autopsy cases of MH.


RESULTS
(1) In all 5 cases, positive reactions of CD45RO, CD3 epsilon, TIA-1, Granzyme B were detected in the neoplastic cells, whereas negative reactions were found with CD30, CD20, CD56. (2) All cases revealed EBER1/2 positivity in neoplastic cells.


CONCLUSIONS
Previously diagnosed malignant histiocytosis is an EBV-associated aggressive NK/T cell lymphoma.
Lu
Changli
C
Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
Liu
Weiping
W
Guo
Jia
J
Li
Gandi
G
Li
Fengyuan
F
D000293:Adolescent; D000328:Adult; D020031:Epstein-Barr Virus Infections / Q000473:pathology*; D005260:Female; D054747:Histiocytic Sarcoma / Q000473:pathology* / Q000821:virology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D016399:Lymphoma, T-Cell / Q000276:immunology; D008297:Male; D016133:Polymerase Chain Reaction