title: Coordinate induction of AMP deaminase in human atrium with mitochondrial DNA deletion.
abstract: Despite the heteroplasmic lower population of mitochondrial (mt) DNA deletion, mtDNA deletion is significantly related to the loss of atrial adenine nucleotides. To elucidate its mechanism, we examined the frequency of a 7.4-kb mtDNA deletion, the concentration of adenine nucleotides, and the activity of AMP catabolic enzymes in 10 human right atria obtained from cardiac surgery, using quantitative PCR, HPLC, and immunoprecipitations. The atrial concentrations of ATP, ADP, AMP, and the total adenine nucleotides were significantly lower in patients with deletion than those in patients without deletion, despite the lower frequency of their deletion. The activities of total AMP deaminase (AMPD), liver-type (AMPD 2), and heart-type isoform (AMPD 3) were significantly higher in patients with deletion than in patients without deletion, although there was no significant difference in the cytosolic 5(')-nucleotidase among them. In conclusion, mtDNA deletion coordinately induces AMP deaminase to contribute to the loss of atrial adenine nucleotides through degrading AMP excessively.
authors.affiliation: Department of Cardiovascular Medicine, Faculty of Medicine, Tottori University, Yonago, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Tomikura
authors.forename: Ichiro
authors.initials: I
authors.lastname: Hisatome
authors.forename: Mariko
authors.initials: M
authors.lastname: Tsuboi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamawaki
authors.forename: Masaki
authors.initials: M
authors.lastname: Shimoyama
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Norihito
authors.initials: N
authors.lastname: Sasaki
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Ogino
authors.forename: Osamu
authors.initials: O
authors.lastname: Igawa
authors.forename: Chiaki
authors.initials: C
authors.lastname: Shigemasa
authors.forename: Shingo
authors.initials: S
authors.lastname: Ishiguro
authors.forename: Shigetsugu
authors.initials: S
authors.lastname: Ohgi
authors.forename: Eiji
authors.initials: E
authors.lastname: Nanba
authors.forename: Goshi
authors.initials: G
authors.lastname: Shiota
authors.forename: Hiroko
authors.initials: H
authors.lastname: Morisaki
authors.forename: Takayuki
authors.initials: T
authors.lastname: Morisaki
authors.forename: Masafumi
authors.initials: M
authors.lastname: Kitakaze
mesh_terms: D000659:AMP Deaminase / Q000096:biosynthesis*; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000502:physiology; D004789:Enzyme Activation; D004790:Enzyme Induction; D017353:Gene Deletion; D006325:Heart Atria / Q000201:enzymology*; D006801:Humans; D008929:Mitochondria, Heart / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; D000659:AMP Deaminase