[Identification of orthopoxvirus species using oligonucleotide micro-chips].
A method for describing the Orthopoxviruses that are pathogenic both to man and animals is described in the article. The method is based on hybridization of a fluorescently labelled amplified DNA sample with oligonucleotides, which were immobilized in a microchip. Species-specific regions within the crmB gene encoding a viral analogue of the tumor necrosis factor receptor, i.e. an important gene determining the pathogenicity of the mentioned Orthopoxviruses type, were used as a target for identification. The identification procedure takes around 6 hours and does not demand any costly equipment (a portable fluorescent microscope can be used).
Mikheev
M V
MV
Lapa
S A
SA
Shchelkunov
S N
SN
Chikova
A K
AK
Mikhaĭlovich
V M
VM
Sobolev
A Iu
AIu
Babkin
I V
IV
Griadunov
D A
DA
Bulavkina
M A
MA
Gus'kov
A A
AA
Sokunova
E B
EB
Kochneva
G V
GV
Blinov
V M
VM
Sandakhchiev
L S
LS
Zasedatelev
A S
AS
Mirzabekov
A D
AD
D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D018155:Orthopoxvirus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics*; D016415:Sequence Alignment; D013045:Species Specificity; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D015345:Oligonucleotide Probes; D018124:Receptors, Tumor Necrosis Factor; D014764:Viral Proteins; C089458:crmB protein, Orthopoxvirus