title: Alkaloid spectrum in diploid and tetraploid hairy root cultures of Datura stramonium.
abstract: Hairy root cultures were obtained from diploid and induced tetraploid plants of Datura stramonium and analyzed by gas chromatography/mass spectrometry. Twenty alkaloids (19 for diploid and 9 for tetraploid hairy root cultures) were identified. A new tropane ester 3-tigloyloxy-6-propionyloxy-7-hydroxytropane was identified on the basis of mass spectral data. Hyoscyamine was the main alkaloid in both diploid and tetraploid cultures. In contrast to diploid hairy roots, the percentage contributions of the alkaloids, with exceptions for hyoscyamine and apoatropine, were higher in the total alkaloid mixture of tetraploid hairy roots.
authors.affiliation: Institute of Botany, Bulgarian Academy Sciences, 23 Acad. G. Bonchev Str., 1113 Sofia, Bulgaria. berkov@iph.bio.bas.bg
authors.forename: Strahil
authors.initials: S
authors.lastname: Berkov
authors.forename: Atanas
authors.initials: A
authors.lastname: Pavlov
authors.forename: Petia
authors.initials: P
authors.lastname: Kovatcheva
authors.forename: Pepa
authors.initials: P
authors.lastname: Stanimirova
authors.forename: Stefan
authors.initials: S
authors.lastname: Philipov
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification; D002478:Cells, Cultured; D013286:Datura stramonium / Q000737:chemistry* / Q000235:genetics; D004171:Diploidy; D008958:Models, Molecular; D008968:Molecular Conformation; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry*; D011123:Polyploidy
chemical_list: D000470:Alkaloids; D010936:Plant Extracts