title: Effect of superovulation on the expression of tissue inhibitor of metalloproteinase-3 in the murine endometrium.
abstract: Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are important in the endometrial remodeling process during embryonic trophoblast invasion. Previous studies, using in situ hybridization, revealed that the predominant proteinase expressed by the implanting mouse embryo is matrix metalloproteinase-9 (MMP-9), and the major tissue inhibitor of metalloproteinases (TIMP) produced in the murine endometrium during this period is TIMP-3. Quantitative, competitive-reverse transcription-PCR was performed to evaluate the expression of TIMP-3 mRNA during the implantation period in the hyperstimulated murine endometrium from pregnant uteri of gestation day (g.d.) 6.0 and 7.0 after injection of 5 and 10 IU pregnant mare serum gonadotropin (PMSG). In PMSG groups, the expression of TIMP-3 mRNA, normalized by MMP-9 mRNA, showed a significant increase compared to the control group (natural coitus without PMSG injection) on g.d. 6.0 (p = 0.039) and an increasing tendency on g.d. 7.0. This study suggests that ovarian hyperstimulation by gonadotropin may have a detrimental effect on embryonic implantation possibly via an altered endometrial remodeling process by the increase in expression of TIMP-3 in the murine endometrium.
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University, Seoul, South Korea. jyhsyk@nuri.net
authors.forename: Seung Yup
authors.initials: SY
authors.lastname: Ku
authors.forename: Young Min
authors.initials: YM
authors.lastname: Choi
authors.forename: Chang Suk
authors.initials: CS
authors.lastname: Suh
authors.forename: Seok Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Jung Gu
authors.initials: JG
authors.lastname: Kim
authors.forename: Shin Yong
authors.initials: SY
authors.lastname: Moon
authors.forename: Jin Yong
authors.initials: JY
authors.lastname: Lee
authors.forename: Yon Su
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000818:Animals; D010064:Embryo Implantation / Q000502:physiology; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006064:Gonadotropins, Equine / Q000008:administration & dosage; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D013480:Superovulation / Q000378:metabolism*; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D006064:Gonadotropins, Equine; D012333:RNA, Messenger; D019717:Tissue Inhibitor of Metalloproteinase-3; D020780:Matrix Metalloproteinase 9