LC determination of salinomycin in fermentation broths and premixes.
A simple and rapid high performance liquid chromatography method for the determination of salinomycin in fermentation media of Streptomyces albus strains and in premixes has been developed. This method involves reverse-phase separation of the component analysed with UV detection at 210 nm using methanol and 0.2 M acetate buffer pH 5.8 (100:10, v/v) as the mobile phase. The reliability of the method was confirmed by validation. A linear relationship was obtained within range 0.2-2.0 mg ml(-1) (r=0.9999). The relative standard deviation of methods within-laboratory reproducibility was 1.6%. The estimated quantitation limit of assay was about 32.5 microg ml(-1). The method has been successfully used in the determination of salinomycin content in testing production processes and premixes of different commercial brands.
Blazsek
Milos
M
R&D, Biotika a.s., SK-976 13 Slovenska Lupca, Slovakia. blazsek@biotika.sk
Surovcová
Anna
A
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005285:Fermentation; D011714:Pyrans / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D000095662:Polyether Polyketides
D000900:Anti-Bacterial Agents; D011714:Pyrans; C010327:salinomycin; D000095662:Polyether Polyketides