[Study on the relationship between human leucocyte antigen and parents' antigen sharing rate and the incidence of pregnancy induced hypertension].
OBJECTIVE
To study the relationship between human leucocyte antigen (HLA), parents' sharing rate and the incidence of pregnancy induced hypertension (PIH), and investigate its immunopathogenetic roles to severe PIH.


METHODS
Newborn's and their parents' peripheral blood DNA was extracted and HLA phenotypes were studied by sequence-specific primers polymerase chain reaction (SSP-PCR) in 57 with PIH and 46 normal pregnancy.


RESULTS
The frequency of DRB1 * 0306, DRB1 * 0405, DRB1 * 0315, DRB1 * 0403, DRB1 * 1304, QB1 * 03032/33, DQB1 * 0502 in women with PIH was increased significantly and the frequency of DRB1 * 0407, DRB1 * 0317, DQB1 * 05031 were lower than controls. And the risk to PIH of women with DRB1 * 0306, DRB1 * 0405, DQB1 * 0502 were higher than others (RR: 9.76, 6.34, 4.20), and its gene-frequency was higher than controls (P < 0.01). Frequency of DQB1 * 0502, DRB1 * 0405 in PIH's newborns were higher than controls (P < 0.05), but DRB1 * 1304, DQB1 * 05031 were lower than controls, with significant difference (P < 0.05); The gene-frequency of fathers of the two groups weren't different. The HLA sharing rate of mothers or fathers of the two groups weren't different. But that of mother-fetus was higher in PIH than controls. Two locus alleles of PIH group constructed haplotype, and the DRB1 * 0315-DQB1 * 0502, DRB1 * 0405-DQB1 * 06012 antibody was positive with hypertension and protenuria (P < 0.05).


CONCLUSIONS
PIH is associated with HLA, and the over expression of HLA genes was a severe cause of PIH.
Liu
Guocheng
G
Shaoxing Women's and Children's Hospital, Zhejiang Province, Shaoxing 312000, China.
Zhang
Xiulan
X
Gou
Wenli
W
D000328:Adult; D005260:Female; D005787:Gene Frequency; D006680:HLA Antigens / Q000235:genetics* / Q000502:physiology; D006801:Humans; D006973:Hypertension / Q000453:epidemiology* / Q000209:etiology; D015994:Incidence; D007231:Infant, Newborn; D008297:Male; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000453:epidemiology* / Q000209:etiology
D006680:HLA Antigens