title: In vitro regeneration of Acacia catechu Willd. from callus and mature nodal explants--an improved method.
abstract: Callus was derived from cultured cotyledons on MS medium supplemented with 2,4-D (0.25 mg/l) and NAA (0.25 mg/l). Plantlets were regenerated from the callus and nodal explants on MS medium containing BAP (2.0 mg/l) and Kn (2.0 mg/l), and further multiplied on the same medium. Addition of adenine sulphate (25.0 mg/l), ascorbic acid (20.0 mg/l) and glutamine (150.0 mg/l) in the medium resulted in enhanced axillary branching. Multiple shoots formed after 6 weeks were separated and subcultured in the fresh medium of same composition. For rhizogenesis, microshoots of 2.0-2.5 cm length were dipped in sterilized IAA solution (10 mg/l) for 24 hr followed by transfer to half strength MS medium containing activated charcoal (0.02%) resulting in rooting (75%) within 8 weeks. The rooted plants were transferred to pots containing sterilized soil and sand mixture for hardening and 71% survival was recorded. Fifty true to type plantlets of A. catechu could be obtained within seven months of culture establishment.
authors.affiliation: Department of Biotechnology, Dr YS Parmar University of Horticulture and Forestry, Nauni Solan 173 230, India.
authors.forename: Manisha
authors.initials: M
authors.lastname: Thakur
authors.forename: D R
authors.initials: DR
authors.lastname: Sharma
authors.forename: Kamlesh
authors.initials: K
authors.lastname: Kanwar
authors.forename: Anil
authors.initials: A
authors.lastname: Kant
mesh_terms: D000045:Acacia / Q000235:genetics / Q000502:physiology*; D046508:Culture Techniques; D012038:Regeneration