Extracting and purifying R-phycoerythrin from Mediterranean red algae Corallina elongata Ellis & Solander.
R-Phycoerythrin (R-PE) is a protein acting as a photosynthetic accessory pigment in red algae (Rodophyta). This protein has gained importance in many biotechnological applications in food science, immunodiagnostic, therapy, cosmetics, protein and cell labelling, and analytical processes. In this paper we report on a new, one step procedure for the extraction and purification of R-PE from a new source: the Mediterranean red algae Corallina elongata Ellis & Solander. This red algae contains mainly R-PE and is suitable for the production in culture. No other contaminating phycobiliproteins could be detected in the extracts. The method we propose for the purification is based on the use of hydroxyapatite, a chromatographic resin that can be produced in the laboratory at very low cost and can be used batch-wise with large amounts of extracts, alternative to chromatography, and therefore can be scaled up. Both the yield and the purity of R-PE are very good.
Rossano
R
R
Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, University of Basilicata, Campus Macchia Romana, I-85000 Potenza, Italy.
Ungaro
N
N
D'Ambrosio
A
A
Liuzzi
G M
GM
Riccio
P
P
D000327:Adsorption; D002845:Chromatography / Q000379:methods*; D017886:Durapatite; D008522:Mediterranean Sea; D010799:Phycoerythrin / Q000096:biosynthesis* / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D000461:Rhodophyta / Q000737:chemistry / Q000378:metabolism*; D013045:Species Specificity
D010799:Phycoerythrin; D017886:Durapatite