Modulation of oxidative stress in response to gamma-radiation in human glioma cell lines.
Radiation therapy is routinely used in the management of primary central nervous system malignancies. However, the efficacy of this therapeutic modality is limited by the occurrence of resistance. In the present study, we investigated whether modulation of oxidative stress might underlie glioma cell radioresistance. Superoxide dismutase activity in irradiated M059J cells was two-fold higher than that in untreated controls, but did not significantly change in U-87 and U-138 cells. This is accompanied by an increase in reactive oxygen species content and decreases in cells viability. Pharmacological or genetic modulation of oxidative stress could be associated with an enhancement in the susceptibility of tumor cells to radiation therapy.
Dal-Pizzol
Felipe
F
Laboratório de Estresse Oxidativo, Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. pizzol@ez-poa.com.br
Ritter
Cristiane
C
Klamt
Fábio
F
Andrades
Michael
M
da Frota
Mário Luís Conte
ML
Diel
Cléber
C
de Lima
Celito
C
Braga Filho
Aroldo
A
Schwartsmann
Gilberto
G
Moreira
José Cláudio Fonseca
JC
D000818:Animals; D001932:Brain Neoplasms / Q000201:enzymology*; D002374:Catalase / Q000378:metabolism; D002470:Cell Survival; D005609:Free Radicals / Q000378:metabolism; D005720:Gamma Rays; D015870:Gene Expression; D005910:Glioma / Q000201:enzymology*; D018384:Oxidative Stress; D011836:Radiation Tolerance; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000528:radiation effects
D005609:Free Radicals; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D013482:Superoxide Dismutase