title: [Determination of sucralose in foods by HPLC using pre-column derivatization].
abstract: The development of a sensitive pre-column derivatization high-performance liquid chromatography (HPLC) method for determination of sucralose is reported. Sucralose is converted into a strongly ultraviolet (UV)-absorbing derivative, possessing strong absorption at 260 nm, by treatment with p-nitrobenzoyl chloride (PNBCl). Homogenized samples were dialyzed and washed with a Bond Elut ENV cartridge, then the eluate was evaporated to dryness and the residue was derivatized. Subsequently, the sucralose derivative was purified with hexane-ethyl actate (9:1) in a silica cartridge, and then the sucralose derivative was eluted with acetone. HPLC was performed on a phenyl column, using acetonitrile-water (73:27) as a mobile phase with UV detection (260 nm). The calibration curve was linear in the range of 1 microgram/mL to 50 micrograms/mL of sucralose. The recoveries of sucralose from eight kinds of foods spiked at the levels of 0.20 and 0.05 g/kg of sucralose were more than 76.2% with SD values in the range from 0.90% to 4.31%. The quantitative limit of the developed method was 0.005 g/kg for sucralose in samples.
authors.affiliation: Tama Branch Laboratory, Tokyo Metropolitan Research Laboratory of Public Health: 3-16-25, Shibasaki-cho, Tachikawa, Tokyo 190-0023, Japan.
authors.forename: Shuko
authors.initials: S
authors.lastname: Nojiri
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Nakazato
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kasuya
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takano
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Oishi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yasuda
authors.forename: Sukeji
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D013395:Sucrose / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry
chemical_list: D013395:Sucrose; C026285:trichlorosucrose