Uptake of radiolabelled alpha-fetoprotein by experimental mammary adenocarcinoma and adenoma: in vivo and in vitro studies.
The biodistribution of iodine-labelled alpha-fetoprotein ( I-AFP) in experimental mammary tumours was studied. C3H mice with subcutaneously transplanted mammary adenocarcinoma and Sprague-Dawley rats treated with -methyl- -nitrosourea for mammary adenoma induction were used as animal models. The accumulation of labelled I-AFP in mouse mammary adenocarcinoma was significantly higher than that in rat mammary adenoma. The tumour/muscle radioactivity ratios increased with time and, 48 h after intravenous injection, were estimated as 23.4 and 6.7, respectively. For experiments, extracts from both mammary tumours were prepared. The extracts were subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to polyvinylidene difluoride (PVDF) membranes and incubated with I-AFP. A single major AFP-binding protein with a molecular weight of about 30 kDa was detected in both extracts. The amount of AFP-binding protein was clearly higher for adenocarcinoma than for adenoma. In the presence of cross-linking reagent, I-AFP formed a complex (about 100 kDa) with adenocarcinoma proteins.
Mirowski
M
M
Radioisotope Centre POLATOM, 05-400 Otwock-S'wierk, Poland. mirowski@ich.pharm.am.lodz.pl
S'Witalska
J
J
Wiercioch
R
R
Byszewska
E
E
Niewiadomska
H
H
Michalska
M
M
D000230:Adenocarcinoma / Q000000981:diagnostic imaging* / Q000473:pathology; D000236:Adenoma / Q000000981:diagnostic imaging* / Q000473:pathology; D000818:Animals; D001692:Biological Transport; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics*; D008325:Mammary Neoplasms, Experimental / Q000000981:diagnostic imaging* / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D011877:Radionuclide Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution; D000509:alpha-Fetoproteins / Q000493:pharmacokinetics*
D007457:Iodine Radioisotopes; D000509:alpha-Fetoproteins