title: Significant improvement of the recombinant Borrelia-specific immunoglobulin G immunoblot test by addition of VlsE and a DbpA homologue derived from Borrelia garinii for diagnosis of early neuroborreliosis.
abstract: We investigated whether the recombinant Borrelia Western blot test previously described (B. Wilske, C. Habermann, V. Fingerle, B. Hillenbrand, S. Jauris-Heipke, G. Lehnert, I. Pradel, D. Rössler, and U. Schulte-Spechtel, Med. Microbiol. Immunol. 188:139-144, 1999) can be improved by the addition of VlsE and additional DbpA and OspC homologues. By using a panel of sera from 36 neuroborreliosis patients and 67 control patients, the diagnostic sensitivity of the recombinant immunoblot test was significantly increased (86.1% versus 52.7%) without loss of specificity and was higher (86.1% versus 63.8%) than that of the conventional whole-cell lysate immunoblot test (U. Hauser, G. Lehnert, R. Lobentanzer, and B. Wilske, J. Clin. Microbiol. 35:1433-1444, 1997). Improvement was mainly due to the presence of VlsE and DbpA.
authors.affiliation: Max von Pettenkofer-Institut für Medizinische Mikrobiologie und Hygiene der Ludwig-Maximilians-Universität München, D-80336 Munich, Germany. Bettina.Wilske@mvp-bak.med.uni-muenchen.de
authors.forename: Ulrike
authors.initials: U
authors.lastname: Schulte-Spechtel
authors.forename: Gisela
authors.initials: G
authors.lastname: Lehnert
authors.forename: Gaby
authors.initials: G
authors.lastname: Liegl
authors.forename: Volker
authors.initials: V
authors.lastname: Fingerle
authors.forename: Christiane
authors.initials: C
authors.lastname: Heimerl
authors.forename: Barbara J B
authors.initials: BJ
authors.lastname: Johnson
authors.forename: Bettina
authors.initials: B
authors.lastname: Wilske
mesh_terms: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology; D015373:Bacterial Typing Techniques; D015748:Borrelia burgdorferi Group / Q000276:immunology / Q000302:isolation & purification*; D053487:DEAD-box RNA Helicases; D019937:Diagnostic Techniques and Procedures; D029968:Escherichia coli Proteins; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007074:Immunoglobulin G; D008074:Lipoproteins / Q000737:chemistry / Q000276:immunology; D020852:Lyme Neuroborreliosis / Q000175:diagnosis* / Q000382:microbiology; D020365:RNA Helicases / Q000737:chemistry / Q000276:immunology; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D007074:Immunoglobulin G; D008074:Lipoproteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; C105546:VlsE protein, Borrelia burgdorferi; C065908:dbpA protein, E coli; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases