The use of lithium chloride to study human milk micelles.
Various methods have been used to study the dissociation of milk micelles in attempts to determine their structure and the interactions that stabilize them. These include the addition of urea, cooling to alter hydrophobic bonding, the addition of EDTA to sequester calcium, and changes in pH to alter molecular charge. For this study, the mild chaotropic agent LiCl was added to human milk micelles, and measurements were made on the relative percentages of the six different phosphorylation levels of beta-casein (CN) at various LiCl concentrations for different lengths of time and at different temperatures. Added LiCl had little effect at 37 degrees C but caused maximal dissociation, mainly of the beta-CN species with higher phosphorylation levels, at 23 degrees C and 4 degrees C between 1 and 2 M concentration. Comparison was made with 2-M additions of NaCl, MgCl2, and KCl at 4 degrees C, with LiCl showing the only appreciable change. The results suggest that Li+ may displace Ca2+ in protein-Ca2+-protein or protein-colloidal calcium phos+ phate-protein salt bridges and that the nonphosphorylated form of human beta-CN may change its conformation and mode of interaction upon phosphorylation. Lithium chloride may be useful to study the dissociation of the different CN in bovine milk micelles.
Sood
S M
SM
Department of Biochemistry and Microbiology, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA. ssood@som.llu.edu
Slattery
C W
CW
D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D002364:Caseins / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D003080:Cold Temperature; D004305:Dose-Response Relationship, Drug; D006801:Humans; D018021:Lithium Chloride / Q000378:metabolism / Q000494:pharmacology*; D008823:Micelles; D008895:Milk, Human / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding; D013997:Time Factors
D002364:Caseins; D008823:Micelles; D018021:Lithium Chloride; D002118:Calcium