Comparison between capillary electrophoresis and high-performance liquid chromatography for the stereoselective analysis of carvedilol in serum.
A high-performance liquid chromatographic (HPLC) assay using a chiral stationary phase was developed and validated for the determination of carvedilol enantiomers in human serum and was compared with a previously developed capillary electrophoresis (CE) method. The CE and the HPLC assay were compared by analyzing a series of serum samples containing racemic carvedilol in different concentrations using the two methods. The concentrations obtained by the two assays were not found to be significantly different indicating that CE and HPLC are comparable in terms of reproducibility and precision for the stereoselective analysis of carvedilol in human serum.
Clohs
Lilian
L
Faculty of Pharmaceutical Sciences, Division of Pharmaceutical Chemistry, University of British Columbia, 2146 East Mall, Vancouver, BC, Canada V6T 1Z3.
McErlane
Keith M
KM
D000319:Adrenergic beta-Antagonists / Q000097:blood*; D002138:Calibration; D002227:Carbazoles / Q000097:blood*; D000077261:Carvedilol; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D011412:Propanolamines / Q000097:blood*; D015203:Reproducibility of Results; D013237:Stereoisomerism
D000319:Adrenergic beta-Antagonists; D002227:Carbazoles; D011412:Propanolamines; D000077261:Carvedilol