Biodegradation of bis(1-chloro-2-propyl) ether via initial ether scission and subsequent dehalogenation by Rhodococcus sp. strain DTB.
Rhodococcus sp. strain DTB (DSM 44534) grows on bis(1-chloro-2-propyl) ether (DDE) as sole source of carbon and energy. The non-chlorinated diisopropyl ether and bis(1-hydroxy-2-propyl) ether, however, did not serve as substrates. In ether degradation experiments with dense cell suspensions, 1-chloro-2-propanol and chloroacetone were formed, which indicated that scission of the ether bond is the first step while dehalogenation of the chlorinated C(3)-compounds occurs at a later stage of the degradation pathway. Inhibition of ether scission by methimazole suggested that the first step in degradation is catalyzed by a flavin-dependent enzyme activity. The non-chlorinated compounds 1,2-propanediol, hydroxyacetone, lactate, pyruvate, 1-propanol, propanal, and propionate also supported growth, which suggested that the intermediates 1,2-propanediol and hydroxyacetone are converted to pyruvate or to propionate, which can be channeled into the citric acid cycle by a number of routes. Total release of chloride and growth-yield experiments with DDE and non-chlorinated C(3)-compounds suggested complete biodegradation of the chlorinated ether.
Moreno Horn
Marcus
M
Fachgebiet Technische Biochemie, Institut für Biotechnologie der Technischen Universität Berlin, Seestrasse 13, 13353, Berlin, Germany.
Garbe
Leif-Alexander
LA
Tressl
Roland
R
Adrian
Lorenz
L
Görisch
Helmut
H
D001673:Biodegradation, Environmental / Q000187:drug effects; D004785:Environmental Pollutants / Q000378:metabolism*; D004986:Ether / Q000032:analysis / Q000378:metabolism*; D004987:Ethers / Q000032:analysis / Q000378:metabolism*; D007220:Industrial Waste / Q000032:analysis; D008713:Methimazole / Q000494:pharmacology; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors; D012240:Rhodococcus / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors
D004785:Environmental Pollutants; D004987:Ethers; D007220:Industrial Waste; C476675:bis(1-chloro-2-propyl) ether; D004986:Ether; D008713:Methimazole; D006899:Mixed Function Oxygenases