Microbial degradation of pyridine using Pseudomonas sp. and isolation of plasmid responsible for degradation.
Pseudomonas (PI2) capable of degrading pyridine was isolated from the mixed population of the activated sludge unit which was being used for treating complex effluents, the strain was characterized. Aerobic degradation of pyridine was studied with the isolated strain and the growth parameters were evaluated. Pyridine degradation was further conformed by chromatography (HPLC) analysis. The process parameters like biomass growth and dissolved oxygen consumption were monitored during pyridine degradation. In order to conform with the plasmid capability to degrade pyridine, the requisite plasmid was isolated and transferred to DH 5alpha Escherichia coli. The subsequent biodegradation studies revealed the ability of the transformed plasmid capability to degrade the pyridine.
Mohan
S Venkata
SV
Biochemical and Environmental Engineering Center, Indian Institute of Chemical Technology, Hyderabad 500007, India.
Sistla
Srinivas
S
Guru
R Kumar
RK
Prasad
K Krishna
KK
Kumar
C Suresh
CS
Ramakrishna
S V
SV
Sarma
P N
PN
D001673:Biodegradation, Environmental; D018533:Biomass; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli; D010101:Oxygen Consumption; D010957:Plasmids / Q000302:isolation & purification / Q000502:physiology*; D011549:Pseudomonas / Q000502:physiology*; D011725:Pyridines / Q000378:metabolism*; D012722:Sewage / Q000737:chemistry / Q000382:microbiology
D011725:Pyridines; D012722:Sewage; C023666:pyridine