title: Tissue window chamber system for validation of implanted oxygen sensors.
abstract: An experimental system is described for validating electrochemical oxygen sensors implanted in tissues. The system is a modified hamster window chamber in which a thin layer of vascularized tissue is held between two plates, one plate having an observation window and the other plate having an array of oxygen sensors. This arrangement permits simultaneous recording of oxygen sensor signals and nondestructive visualization of the tissue adjacent to the sensors over periods of 1 mo or more, without the inhibitory effects of anesthesia. The system provides a means for study of the effects of spatial and temporal oxygen distributions on the sensor signals and adaptation of the tissue structure over time. Examples are given of sensor recordings and images of tissues with implanted oxygen sensor arrays.
authors.affiliation: Department of Bioengineering, University of California-San Diego, La Jolla 92093-0412, USA.
authors.forename: Milan T
authors.initials: MT
authors.lastname: Makale
authors.forename: Joe T
authors.initials: JT
authors.lastname: Lin
authors.forename: Richard E
authors.initials: RE
authors.lastname: Calou
authors.forename: Amy G
authors.initials: AG
authors.lastname: Tsai
authors.forename: Peter C
authors.initials: PC
authors.lastname: Chen
authors.forename: David A
authors.initials: DA
authors.lastname: Gough
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002138:Calibration; D006224:Cricetinae; D004563:Electrochemistry; D004567:Electrodes, Implanted; D008297:Male; D008647:Mesocricetus; D010092:Oximetry / Q000295:instrumentation*; D010101:Oxygen Consumption / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology