[Evaluation on the number and value of STR loci applied in paternity identification].
OBJECTIVE
The study was carried out on the evaluation of number and value of STR loci applied in paternity identification.


METHODS
A total of 13 STR loci, divided into four groups, was observed in 102 cases of paternity exclusion and 100 cases of paternity inclusion. PCR amplified products of 13 STR loci were injected into a capillary on the ABI PRISM 310 Genetic Analyzer. GeneScan software analyzed the collected data, which can then be imported into Genotyper software for genotyping of alleles.


RESULTS
At least 3 STR loci incompatibilities between alleged father and child were found in all paternity exclusion cases of two observed groups which Cumulative Probability of Exclusion (CPE) was more than 99.99%, and in all paternity inclusion cases of those same observed groups, their Relative Chance of Paternity(RCP) could be over 99.99%.


CONCLUSIONS
The exclusion of paternity should be based on at least three STR loci incompatibilities in the identification practice. As a criterion for evaluation of the number and value of STR loci applied in paternity test, CPE should reach 99.99%.
Fang
Jian-xin
JX
Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063.
Cheng
Da-lin
DL
D000328:Adult; D002648:Child; D016172:DNA Fingerprinting; D005260:Female; D005554:Forensic Medicine; D005787:Gene Frequency; D006801:Humans; D008297:Male; D010334:Paternity; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences / Q000235:genetics*