Improved anaerobic use of arginine by Saccharomyces cerevisiae.
Anaerobic arginine catabolism in Saccharomyces cerevisiae was genetically modified to allow assimilation of all four rather than just three of the nitrogen atoms in arginine. This was accomplished by bypassing normal formation of proline, an unusable nitrogen source in the absence of oxygen, and causing formation of glutamate instead. A pro3 ure2 strain expressing a PGK1 promoter-driven PUT2 allele encoding Delta(1)-pyrroline-5-carboxylate dehydrogenase lacking a mitochondrial targeting sequence produced significant cytoplasmic activity, accumulated twice as much intracellular glutamate, and produced twice as much cell mass as the parent when grown anaerobically on limiting arginine as sole nitrogen source.
Martin
Olga
O
Department of Food Science and Technology, Oregon State University, Corvallis, OR 97331-6602, USA.
Brandriss
Marjorie C
MC
Schneider
Gisbert
G
Bakalinsky
Alan T
AT
D000693:Anaerobiosis; D001120:Arginine / Q000378:metabolism*; D003470:Culture Media; D015966:Gene Expression Regulation, Fungal; D005818:Genetic Engineering / Q000379:methods*; D018698:Glutamic Acid / Q000378:metabolism; D005979:Glutathione Peroxidase; D009584:Nitrogen / Q000378:metabolism; D011328:Prions / Q000235:genetics / Q000378:metabolism; D011762:Pyrroline Carboxylate Reductases / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D000097502:delta-1-Pyrroline-5-Carboxylate Reductase
D001120:Arginine; D003470:Culture Media; D018698:Glutamic Acid; D005979:Glutathione Peroxidase; D009584:Nitrogen; D011328:Prions; D011762:Pyrroline Carboxylate Reductases; D029701:Saccharomyces cerevisiae Proteins; D000097502:delta-1-Pyrroline-5-Carboxylate Reductase; C067020:URE2 protein, S cerevisiae