title: CD34-positive cells in human umbilical cord blood express nerve growth factor and its specific receptor TrkA.
abstract: In this study, we investigated whether hematopoietic stem cells (HSC) and progenitors present in human cord blood can express nerve growth factor (NGF)-specific receptors, TrkA and p75. Our results showed a marked expression of TrkA and NGF in cord blood CD34(+) cells. A gradient of TrkA and NGF expression exists and is highest in cord blood CD34(+) cells, reduced in cord blood mononuclear cells (MNC) and minimal in mononuclear cells isolated from adult peripheral blood. Our findings suggest that NGF may play a role in the differentiation of hematopoietic progenitors and indicate a different requirement for NGF by immune cells, depending on their state of maturity.
authors.affiliation: Institute of Neurobiology and Molecular Medicine, CNR, Viale Marx 15/43, 00137 Rome, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Bracci-Laudiero
authors.forename: Domenico
authors.initials: D
authors.lastname: Celestino
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Starace
authors.forename: Alessia
authors.initials: A
authors.lastname: Antonelli
authors.forename: Alessandro
authors.initials: A
authors.lastname: Lambiase
authors.forename: Annabella
authors.initials: A
authors.lastname: Procoli
authors.forename: Carlo
authors.initials: C
authors.lastname: Rumi
authors.forename: Marco
authors.initials: M
authors.lastname: Lai
authors.forename: Alessandra
authors.initials: A
authors.lastname: Picardi
authors.forename: Giovanna
authors.initials: G
authors.lastname: Ballatore
authors.forename: Sergio
authors.initials: S
authors.lastname: Bonini
authors.forename: Luigi
authors.initials: L
authors.lastname: Aloe
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology*; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008875:Middle Aged; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020800:Receptor, Nerve Growth Factor; D020917:Receptor, trkA / Q000235:genetics / Q000378:metabolism*; D017475:Receptors, Nerve Growth Factor / Q000235:genetics / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D012333:RNA, Messenger; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor; D020932:Nerve Growth Factor; D020917:Receptor, trkA