title: Distribution of Aeromonas hydrophila serogroups in different clinical samples and the development of polyclonal antibodies for rapid identification of the genus Aeromonas by direct agglutination.
abstract: We characterized a collection of 256 Aeromonas hydrophila strains isolated from blood, discharge and stool for their serogroup designation. Of these, 2.3% were untypable and 15.2% were rough strains. Among the typable strains, about 50% comprised serogroups O:11, O:16, O:18, O:34 and O:83. To develop rapid differentiation of Aeromonas from other oxidase-positive bacteria, antisera against Aeromonas were produced to establish a direct, genus-specific, agglutination test. It was found that among 105 isolates of Aeromonas, 102 showed positive results with the agglutination test. The calculated sensitivity and specificity were 97.1% and 90.7%, respectively.
authors.affiliation: Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. grsks@mahidol.ac.th
authors.forename: Sunee
authors.initials: S
authors.lastname: Korbsrisate
authors.forename: Suwat
authors.initials: S
authors.lastname: Dumnin
authors.forename: Rungrawan
authors.initials: R
authors.lastname: Chawengkirttikul
authors.forename: Vanchit
authors.initials: V
authors.lastname: Gherunpong
authors.forename: Boonchuay
authors.initials: B
authors.lastname: Eampokalap
authors.forename: Charles
authors.initials: C
authors.lastname: Gongviseisoog
authors.forename: Kritsana
authors.initials: K
authors.lastname: Janyapoon
authors.forename: Kancharee
authors.initials: K
authors.lastname: Lertpocasombat
authors.forename: Toshio
authors.initials: T
authors.lastname: Shimada
mesh_terms: D000333:Aeromonas / Q000276:immunology / Q000302:isolation & purification*; D016980:Aeromonas hydrophila / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D000372:Agglutination Tests / Q000379:methods*; D000907:Antibodies, Bacterial / Q000097:blood*; D015153:Blotting, Western / Q000379:methods; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis*; D006801:Humans; D019081:O Antigens / Q000032:analysis; D012703:Serotyping
chemical_list: D000907:Antibodies, Bacterial; D019081:O Antigens