[The expression of FN-IIIcs in the wound healing and its relationship with injury time].
OBJECTIVE
To research the expression of alternative splicing segment of fibronectin-IIIcs in incised wound of skin, to offer the experimental data for early injury time judgement.


METHODS
Using in situ hybridization with DIG-labeled anti-sense RNA probe, the expression of FN-IIIcs domain was detected in both human and rat skin incised wound.


RESULTS
(1) The expression of FN-IIIcs improved from the time of 30 min after injury, reached peak at 6 h and then reduced gradually. (2) The positive expression cells were mainly distributed in hair follicles, sebaceous glands and endothelium, however, in human beings they were basement cells of epidermis.


CONCLUSIONS
The expression of FN-IIIcs domain would be a supposed useful criteria for early injury time judgement.
Ji
Yan
Y
Department of Forensic Medicine of Fudan University, Shanghai 200032.
Zhao
Zi-qin
ZQ
Gu
Yun-ju
YJ
D000818:Animals; D005353:Fibronectins / Q000096:biosynthesis* / Q000235:genetics; D005554:Forensic Medicine; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries* / Q000378:metabolism*; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*
D005353:Fibronectins; D012333:RNA, Messenger