title: Angiotensin I-converting enzyme inhibitory peptides in a hydrolyzed chicken breast muscle extract.
abstract: The blood pressure of spontaneously hypertensive rats (SHRs) decreased after oral administration of an extract prepared from chicken breast muscle, falling maximally to 50 mmHg lower than before. This effect continued for at least 4 h after administration. The peptides possessing hypotensive activity in the chicken extract were examined by measuring the inhibitory activity (IC(50)) against angiotensin I-converting enzyme (ACE). The inhibitory activity of the chicken extract was 1060 mg%, whereas the activity of the extract treated with an Aspergillus protease and gastric proteases (trypsin, chymotrypsin, and intestinal juice) became stronger, reaching 1.1 mg%. Peptides in this hydrolysate of the extract were isolated by HPLC on a reversed-phase column, and their N-terminal sequences were analyzed. Three peptides possessed a common sequence, Gly-X-X-Gly-X-X-Gly-X-X, which was homologous with that of collagen. The peptide Gly-Phe-Hyp-Gly-Thr-Hyp-Gly-Leu-Hyp-Gly-Phe showed the strongest inhibitory activity (IC(50) = 42 microM).
authors.affiliation: Graduate School of Biosphere, Hiroshima University, Higashi-hiroshima 739-8528, Japan.
authors.forename: Ai
authors.initials: A
authors.lastname: Saiga
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Okumura
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Makihara
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Katsuta
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Ryoji
authors.initials: R
authors.lastname: Yamada
authors.forename: Toshihide
authors.initials: T
authors.lastname: Nishimura
mesh_terms: D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis* / Q000494:pharmacology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D010450:Endopeptidases / Q000378:metabolism; D006868:Hydrolysis; D007421:Intestine, Small / Q000737:chemistry; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry*; D009842:Oligopeptides / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D010455:Peptides / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D011918:Rats, Inbred SHR; D013552:Swine; D014020:Tissue Extracts / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D009842:Oligopeptides; D010455:Peptides; D014020:Tissue Extracts; D010450:Endopeptidases