title: Study of the spontaneous dissociation of rabbit C-reactive protein.
abstract: C-Reactive protein (CRP) is composed of five identical noncovalently linked monomers and characterized as an important acute-phase protein. The CRP subunit obtained by denaturing treatments, which is termed modified CRP, has also been widely studied. In the current work, we found that there exists some degree of natural dissociation of CRP in stock solution. This dissociation is critically dependent on the absence of Ca2+. Low pH could enhance the dissociation of CRP, while ionic strength has little effect. Anilinonaphthalenesulfonate (ANS) fluorescence detections indicate that the exposure of hydrophobic surface increases during the dissociation. Acidic pH conditions also induce an increase in ANS fluorescence. This suggests that hydrophobic interactions between CRP subunits may contribute to the study of its pentameric structure. Surface plasmon resonance experiments indicate that monomeric CRP does not specifically bind to phosphatidylcholine-containing membrane as native CRP does. Electron microscopy shows that monomeric CRP binds to negatively charged lipid through electrostatic forces, and such lipid may induce the dissociation of CRP due to the acidic pH in the diffuse double layer near the membrane.
authors.affiliation: Department of Biological Sciences and Biotechnology, State-Key Laboratory of Biomembranes, Tsinghua University, Beijing 100084, Peoples' Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: S-R
authors.initials: SR
authors.lastname: Ji
authors.forename: H-W
authors.initials: HW
authors.lastname: Wang
authors.forename: S-F
authors.initials: SF
authors.lastname: Sui
mesh_terms: D000817:Anilino Naphthalenesulfonates / Q000494:pharmacology; D000818:Animals; D002097:C-Reactive Protein / Q000737:chemistry* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D011817:Rabbits; D020349:Surface Plasmon Resonance; D013997:Time Factors
chemical_list: D000817:Anilino Naphthalenesulfonates; D008055:Lipids; D002097:C-Reactive Protein; D002118:Calcium