title: Effects of direct hemoperfusion with a beta2-microglobulin adsorption column on hypercytokinemia in rats.
abstract: OBJECTIVE
  Blood purification therapies have been clinically applied to treat the cytokine-induced pathological responses. The effects of direct hemoperfusion(DHP) with a beta(2)-microglobulin (BMG) adsorption column on hypercytokinemia were investigated.
  
  
  METHODS
  A hypercytokinemia model was prepared by injecting lipopolysaccharide (LPS) into femoral veins of male Wistar rats weighing 250-400 g. The hypercytokinemic rats were connected to the DHP system using a small BMG adsorption column, and time course changes in plasma levels of inflammatory cytokines were then examined.
  
  
  RESULTS
  Plasma levels of interleukins (IL) 1beta and 6 and tumor necrosis factor alpha (TNF-alpha) were found to be significantly elevated within 3 h after LPS injection in the control group that underwent extracorporeal circulation without the BMG adsorption column, whereas the increases in plasma levels of IL-6 and TNF-alpha were significantly inhibited in the group that was treated with the BMG adsorption column. The adsorption rates of cytokines with the BMG adsorption column were 20-40% for IL-1beta and IL-6 and 10-85% for TNF-alpha during treatment for 3 h.
  
  
  CONCLUSIONS
  These results suggest that the systemic inflammatory response syndrome with hypercytokinemia might be treated with DHP using the BMG adsorption column.
authors.affiliation: Department of Urology and Division of Artificial Kidney, Osaka City University Medical School, Osaka, Japan. nakatani@misc.med.osaka-cu.ac.jp
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nakatani
authors.forename: Kenji
authors.initials: K
authors.lastname: Tsuchida
authors.forename: Ontaku
authors.initials: O
authors.lastname: Fu
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Sugimura
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Takemoto
mesh_terms: D000327:Adsorption; D000818:Animals; D002853:Chromatography, Liquid; D019446:Endotoxemia / Q000097:blood / Q000139:chemically induced / Q000628:therapy; D006464:Hemoperfusion / Q000295:instrumentation; D015850:Interleukin-6 / Q000097:blood*; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood / Q000139:chemically induced / Q000628:therapy*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*; D001613:beta 2-Microglobulin
chemical_list: D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D001613:beta 2-Microglobulin