title: T cells infiltrate the brain in murine and human transmissible spongiform encephalopathies.
abstract: CD4 and CD8 T lymphocytes infiltrate the parenchyma of mouse brains several weeks after intracerebral, intraperitoneal, or oral inoculation with the Chandler strain of mouse scrapie, a pattern not seen with inoculation of prion protein knockout (PrP(-/-)) mice. Associated with this cellular infiltration are expression of MHC class I and II molecules and elevation in levels of the T-cell chemokines, especially macrophage inflammatory protein 1beta, IFN-gamma-inducible protein 10, and RANTES. T cells were also found in the central nervous system (CNS) in five of six patients with Creutzfeldt-Jakob disease. T cells harvested from brains and spleens of scrapie-infected mice were analyzed using a newly identified mouse PrP (mPrP) peptide bearing the canonical binding motifs to major histocompatibility complex (MHC) class I H-2(b) or H-2(d) molecules, appropriate MHC class I tetramers made to include these peptides, and CD4 and CD8 T cells stimulated with 15-mer overlapping peptides covering the whole mPrP. Minimal to modest K(b) tetramer binding of mPrP amino acids (aa) 2 to 9, aa 152 to 160, and aa 232 to 241 was observed, but such tetramer-binding lymphocytes as well as CD4 and CD8 lymphocytes incubated with the full repertoire of mPrP peptides failed to synthesize intracellular gamma interferon (IFN-gamma) or tumor necrosis factor alpha (TNF-alpha) cytokines and were unable to lyse PrP(-/-) embryo fibroblasts or macrophages coated with (51)Cr-labeled mPrP peptide. These results suggest that the expression of PrP(sc) in the CNS is associated with release of chemokines and, as shown previously, cytokines that attract and retain PrP-activated T cells and, quite likely, bystander activated T cells that have migrated from the periphery into the CNS. However, these CD4 and CD8 T cells are defective in such an effector function(s) as IFN-gamma and TNF-alpha expression or release or lytic activity.
authors.affiliation: Division of Virology, Department of Neuropharmacology (IMM-6), The Scripps Research Institute, La Jolla, California 92037, USA.
authors.forename: Hanna
authors.initials: H
authors.lastname: Lewicki
authors.forename: Antoinette
authors.initials: A
authors.lastname: Tishon
authors.forename: Dirk
authors.initials: D
authors.lastname: Homann
authors.forename: Honoré
authors.initials: H
authors.lastname: Mazarguil
authors.forename: Françoise
authors.initials: F
authors.lastname: Laval
authors.forename: Valerie C
authors.initials: VC
authors.lastname: Asensio
authors.forename: Iain L
authors.initials: IL
authors.lastname: Campbell
authors.forename: Stephen
authors.initials: S
authors.lastname: DeArmond
authors.forename: Bryan
authors.initials: B
authors.lastname: Coon
authors.forename: Chao
authors.initials: C
authors.lastname: Teng
authors.forename: Jean Edouard
authors.initials: JE
authors.lastname: Gairin
authors.forename: Michael B A
authors.initials: MB
authors.lastname: Oldstone
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D007562:Creutzfeldt-Jakob Syndrome / Q000276:immunology*; D006183:H-2 Antigens / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D018620:PrPSc Proteins / Q000737:chemistry / Q000378:metabolism; D012608:Scrapie / Q000276:immunology*
chemical_list: D006183:H-2 Antigens; D010455:Peptides; D018620:PrPSc Proteins