Expression of B-lymphocyte-associated transcription factors in human T-cell neoplasms.
In this study we have investigated the expression of three B-cell-associated transcription factors in normal lymphoid tissue and in T-cell neoplasms (three cell lines, and more than 50 biopsy samples). Nuclear OCT-1 immunoreactivity was seen in normal B cells, in many extrafollicular T cells, and in a heterogeneous pattern (ranging in intensity from weak to moderate) in most T-cell neoplasms. OCT-2 immunostaining was primarily restricted in normal lymphoid tissue to B cells, and was absent from most T-cell neoplasms. In contrast, immunostaining for BOB-1/OCA-B--essentially restricted to B cells in normal lymphoid tissue, with the exception of activated T-lymphocytes--was seen in all of the T-cell lines tested and the majority of the tumor cells in all categories of T-cell lymphoma. Thus labeling for each of these three B-cell-associated transcription factors can be seen to varying degrees in T-cell neoplasms. However, the high frequency of BOB-1 expression in T-cell neoplasms, in contrast to its absence from resting peripheral T cells, suggests that its expression might be a prerequisite for neoplastic transformation, and prompts a search for the transcriptional target(s) of this factor in T cells.
Marafioti
Teresa
T
Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Oxford, United Kingdom. teresa.marafioti@ndcls.ox.ac.uk
Ascani
Stefano
S
Pulford
Karen
K
Sabattini
Elena
E
Piccioli
Milena
M
Jones
Margaret
M
Zinzani
Pier Luigi
PL
Delsol
Georges
G
Mason
David Y
DY
Pileri
Stefano A
SA
D000951:Antigens, Neoplasm / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D001706:Biopsy; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics; D051863:Host Cell Factor C1; D006801:Humans; D007150:Immunohistochemistry; D019169:Jurkat Cells; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; C497692:HCFC1 protein, human; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; C494165:POU2F1 protein, human; C058435:POU2F2 protein, human; D014157:Transcription Factors