title: Successful ex vivo normothermic liver perfusion with purely artificial products using artificial blood.
abstract: We tried to make an ex vivo functioning liver with an artificial perfusate that consisted of artificial blood in the pig liver. A liver graft from a female pig weighing 20 kg was harvested in the usual manner. The perfusion solution consisted of artificial blood, L-15 medium, distilled water, bovine serum albumin, NaHCO3, NaOH, KCl, human regular insulin, 50% glucose solution, and dexamethasone. The isolated liver was perfused with this oxygenated perfusate through the portal vein at a rate of 300 ml/min for 9 hours. Seven livers were perfused for 9 hours in this system. Five of the livers showed mean oxygen consumption of over 8 ml-O2/min during perfusion. Histological findings showed that the hepatic architecture was almost completely preserved and numerous hepatocytes exhibited PAS-positive cytoplasmic glycogen deposits in these livers. These observations indicate that we have succeeded in developing an ex vivo functioning liver with an artificial perfusate employing artificial blood.
authors.affiliation: First Department of Surgery, Sapporo Medical University, School of Medicine, Sapporo, Japan. katsuram@sapmed.ac.jp
authors.forename: A
authors.initials: A
authors.lastname: Nui
authors.forename: T
authors.initials: T
authors.lastname: Katsuramaki
authors.forename: H
authors.initials: H
authors.lastname: Kikuchi
authors.forename: K
authors.initials: K
authors.lastname: Kukita
authors.forename: M
authors.initials: M
authors.lastname: Nagayama
authors.forename: M
authors.initials: M
authors.lastname: Meguro
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: M
authors.initials: M
authors.lastname: Isobe
authors.forename: K
authors.initials: K
authors.lastname: Hirata
mesh_terms: D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology; D005260:Female; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D066298:In Vitro Techniques; D008099:Liver / Q000098:blood supply* / Q000166:cytology / Q000187:drug effects; D008102:Liver Circulation; D010101:Oxygen Consumption / Q000187:drug effects; D013552:Swine
chemical_list: D001802:Blood Substitutes