title: Purification, crystallization and preliminary X-ray analysis of Caenorhabditis elegans ubiquitin-conjugation enzyme M7.1.
abstract: M7.1 is a class IV ubiquitin-conjugation enzyme (UBC) that belongs to the ubiquitination cascade in Caenorhabditis elegans. The clone for this UBC has been overexpressed in Escherichia coli and the 16.7 kDa protein was purified from the soluble fraction. M7.1 was crystallized by sitting-drop vapor diffusion in 10% ethanol, 1.5 M NaCl at 277.5 K. Crystals diffracted to 1.75 A and belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 44.3, b = 54.3, c = 60.2 A. The asymmetric unit contains a single monomer. A molecular-replacement model has been determined and refinement is in progress.
authors.affiliation: Laboratory for Structural Biology, The University of Alabama in Huntsville, Huntsville, Alabama, USA.
authors.forename: José A
authors.initials: JA
authors.lastname: Gavira J
authors.forename: Derek P
authors.initials: DP
authors.lastname: Claxton
authors.forename: Jenny H
authors.initials: JH
authors.lastname: Wang
authors.forename: Diana
authors.initials: D
authors.lastname: Toh
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Meehan
authors.forename: Enrico L
authors.initials: EL
authors.lastname: DiGiammarino
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000201:enzymology*; D029742:Caenorhabditis elegans Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D008025:Ligases / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044763:Ubiquitin-Conjugating Enzymes
chemical_list: D029742:Caenorhabditis elegans Proteins; D044763:Ubiquitin-Conjugating Enzymes; C063110:let-70 protein, C elegans; D008025:Ligases