title: Contrast media augmented apoptosis of cultured renal mesangial, tubular, epithelial, endothelial, and hepatic cells.
abstract: OBJECTIVE
  Nephrotoxicity of contrast media, resulting in apoptosis and acute necrosis of tubular cells, is well documented. No studies concerning mesangial cells apoptosis have been published yet.
  
  
  OBJECTIVE
  Apoptosis of cultured mesangial, tubular, and hepatic cell lines was investigated following exposure to different contrast media.
  
  
  METHODS
  Apoptosis was assessed by TUNEL assay and verified by Mayer Hematoxylin staining.
  
  
  RESULTS
  Iopromide, Ioxaglate, and Ioxatalamate induced apoptosis in all cell cultures at final concentrations ranged from 0.1% to 10.0%. However, only 1% to 10% Iomeprol elicited a significant apoptosis. Moreover, at 10% concentration, Iomeprol induced significantly less apoptosis than Iopromide, Ioxaglate, or Ioxatalamate.
  
  
  CONCLUSIONS
  First, Iomeprol, which has a different physico-chemical properties, proved to be less proapoptotic compared with other contrast compounds. Second, all types of cells similarly respond by apoptosis to contrast media induced injury. However, apoptosis of mesangial cells might generate additional deleterious effects in vivo.
authors.affiliation: Nephrology Division and the Department of Interventional Radiology, Assaf Harofeh Medical Center, Zerifin, Israel.
authors.forename: Amir
authors.initials: A
authors.lastname: Peer
authors.forename: Zhan
authors.initials: Z
authors.lastname: Averbukh
authors.forename: Sylvia
authors.initials: S
authors.lastname: Berman
authors.forename: David
authors.initials: D
authors.lastname: Modai
authors.forename: Michael
authors.initials: M
authors.lastname: Averbukh
authors.forename: Joshua
authors.initials: J
authors.lastname: Weissgarten
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D003287:Contrast Media / Q000633:toxicity*; D004730:Endothelium, Vascular / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects; D005920:Glomerular Mesangium / Q000187:drug effects*; D007684:Kidney Tubules / Q000166:cytology / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D014283:Triiodobenzoic Acids / Q000633:toxicity
chemical_list: D003287:Contrast Media; D014283:Triiodobenzoic Acids