Overexpression and increased DNA topoisomerase II-like enzyme activity in arsenite resistant Leishmania donovani.
Western immunoblot analyses of whole cell lysates probed with a human specific monoclonal anti-topoisomerase IIalpha antibody identified a 190 kDa protein over expressed in the arsenite resistant Leishmania donovani strain. The crude nuclear extract of the resistant strain showed higher topoisomerase II-like enzyme activity. suggesting a possible regulatory role of putative topoisomerase II in arsenite resistant Leishmania.
Jayanarayan
K G
KG
Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar, Punjab 160062, India.
Dey
C S
CS
D000818:Animals; D001152:Arsenicals / Q000494:pharmacology*; D015153:Blotting, Western; D004250:DNA Topoisomerases, Type II / Q000235:genetics* / Q000378:metabolism; D004351:Drug Resistance / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007893:Leishmania donovani / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D009675:Novobiocin / Q000494:pharmacology
D001152:Arsenicals; D009675:Novobiocin; D004250:DNA Topoisomerases, Type II