[Antisense TIF3 reverses the oncogenic potential of CdCl2-transformed BALB/c-3T3 cells].
TIF3 (GenBank Accession Number AF 271072) is identified as a novel cadmium- responsive proto-oncogene. In order to determine whether the antisense TIF3 reverses the oncogenic potential of Cd-transformed BALB/c-3T3 cells or not, a stable expression system of CdCl2-transformed BALB/c-3T3 cells with the expression vector containing TIF3 cDNA in the antisense orientation using calcium phosphate and G418 selection protocols is firstly established. Then, the reversal of the oncogenic potential of these cells is tested by soft agar and nude mouse tumorigenicity assay. The results demonstrated that expression of the antisense TIF3 in the CdCl2-transformed BALB/c-3T3 cells results in reversal of the transformed phenotype of the cells. This is evidenced by a 25%-70% decrease in the number of anchorage-independent colonies growing on soft agar and the significant reduced tumorigenic potential of cells in nude mice compared with the corresponding controls. In addition to a significant delay in the onset of appearance of tumors, a significant reduction in size and a 50.8%-55.1% decrease in weight of the tumors are also observed in the mice injected with the TIF3 antisense expressing cells compared with the corresponding controls. The results indicate that antisense TIF3 mRNA expression reverses its oncogenic potential of Cd-transformed BALB/c-3T3 cells and may have therapeutic potential to cancer induced by cadmium.
Lei
Yixiong
Y
Institute for Chemical Carcinogenesis, Guangzhou Medical College, Guangzhou 510182, China.
Pius
Joseph
J
Wu
Zhongliang
Z
Ong
Tong-man
TM
D016475:3T3 Cells / Q000166:cytology*; D000818:Animals; D016375:Antisense Elements (Genetics); D019256:Cadmium Chloride / Q000633:toxicity*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010448:Peptide Initiation Factors / Q000235:genetics / Q000494:pharmacology*; D011519:Proto-Oncogenes / Q000235:genetics; D014162:Transfection
D016375:Antisense Elements (Genetics); D010448:Peptide Initiation Factors; D019256:Cadmium Chloride