Effects of hyperthermia and differentiation on cultured Dunn osteosarcoma cells.
We investigated the characteristics of morphology, DNA synthesis and argyrophilic nucleolar organizer regions (AgNORs) on murine Dunn osteosarcoma cells in response to heat (42 degrees C, 1 h) or dibutyryl cyclic adenosine 3',5'-monophosphate (Bt(2)cAMP). The cell morphology changes to a fibroblast-like appearance with long and thin protoplastic processes with the reduction of DNA synthesis by heat. It is closely similar to a response by Bt(2)cAMP. In the presence of 3 mM Bt(2)cAMP, the mean number of AgNORs was significantly decreased in 48 h compared with the untreated group. It was increased conversely by heat. Among these responsive cells, we can also find many cells stained without discrimination by the use of AgNORs staining. The present study provides a new clue to support differentiation of osteosarcoma cells from the viewpoint of hyperthermia in vitro.
Kanamori
Masahiko
M
Department of Orthopaedic Surgery, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan. kanamori@ms.toyama-mpu.ac.jp
Ohmori
Kazudo
K
Yasuda
Taketoshi
T
Yudoh
Kazuo
K
D000818:Animals; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002466:Cell Nucleolus / Q000187:drug effects / Q000235:genetics / Q000648:ultrastructure; D004273:DNA, Neoplasm / Q000032:analysis / Q000187:drug effects / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005260:Female; D006979:Hyperthermia, Induced; D051379:Mice; D009697:Nucleolus Organizer Region / Q000187:drug effects* / Q000235:genetics; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D003994:Bucladesine