Biocatalytic deprotection of a cetraxate ester by Microbacterium sp. strain 7-1W cells.
Enzymatic deprotection of the terminal ester bond of a cetraxate methyl ester was done with resting cells of Microbacterium sp. strain 7-1W, which produces an esterase catalyzing a regioselective hydrolysis reaction, as the catalyst. When 20 g of cetraxate methyl ester in 50 ml of a reaction mixture was incubated with 5 g of wet cells for 17 h, 96% of the substrate was converted to the desired product, cetraxate, quantitatively.
Honda
Kohsuke
K
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Sakamoto
Keiji
K
Kita
Shinji
S
Kataoka
Michihiko
M
Shimizu
Sakayu
S
D002384:Catalysis; D004950:Esterases / Q000737:chemistry*; D004952:Esters / Q000378:metabolism; D005374:Filtration; D006094:Gram-Positive Bacteria / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D013696:Temperature; D014148:Tranexamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry*
D004952:Esters; C000665:cetraxate; D014148:Tranexamic Acid; D004950:Esterases