title: Myosin isoforms show unique conformations in the actin-bound state.
abstract: Crystallographic data for several myosin isoforms have provided evidence for at least two conformations in the absence of actin: a prehydrolysis state that is similar to the original nucleotide-free chicken skeletal subfragment-1 (S1) structure, and a transition-state structure that favors hydrolysis. These weak-binding states differ in the extent of closure of the cleft that divides the actin-binding region of the myosin and the position of the light chain binding domain or lever arm that is believed to be associated with force generation. Previously, we provided insights into the interaction of smooth-muscle S1 with actin by computer-based fitting of crystal structures into three-dimensional reconstructions obtained by electron cryomicroscopy. Here, we analyze the conformations of actin-bound chicken skeletal muscle S1. We conclude that both myosin isoforms in the nucleotide-free, actin-bound state can achieve a more tightly closed cleft, a more downward position of the lever arm, and more stable surface loops than those seen in the available crystal structures, indicating the existence of unique actin-bound conformations.
authors.affiliation: The Burnham Institute, La Jolla, CA 92037, USA.
authors.forename: Niels
authors.initials: N
authors.lastname: Volkmann
authors.forename: Greta
authors.initials: G
authors.lastname: Ouyang
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Trybus
authors.forename: David J
authors.initials: DJ
authors.lastname: DeRosier
authors.forename: Susan
authors.initials: S
authors.lastname: Lowey
authors.forename: Dorit
authors.initials: D
authors.lastname: Hanein
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D020285:Cryoelectron Microscopy; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000737:chemistry / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D009130:Muscle, Smooth / Q000378:metabolism; D015879:Myosin Subfragments / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D000199:Actins; D020409:Molecular Motor Proteins; D015879:Myosin Subfragments; D020033:Protein Isoforms