The transfection of Jurkat T-leukemic cells by use of pH-sensitive immunoliposomes.
An effective pH-sensitive gene transfer vector has been developed utilising anionic liposomes with various formulations as a carrier of plasmid DNA (pEGlacZ, 7.6 kb) to transfect CD3 T+ lymphocytes (Jurkat cells). The plasmid DNA was condensed using poly-l-lysines with a range of molecular masses to form polyplexes that were interacted with several anionic liposome formulations to form lipopolyplexes. For targeting to the Jurkat cells, distearoylphosphatidylethanolamine (DSPE) linked to poly (ethylene glycol) molecular mass 2000 and coupled to anti-CD3 antibody was incorporated in the liposomes. The polyplexes and lipopolyplexes were characterised in terms of size, zeta potential, gel electrophoresis and electron microscopy. The gene transfer activity of the lipopolyplexes, assessed from beta-galactosidase expression, depended on the charge ratio (NH(3)+/PO(4)-) of the component polyplex and the lipid/DNA weight ratio of the lipopolyplex.
Turner
Christopher
C
School of Biological Sciences, University of Manchester, Manchester, M13 9PL, UK.
Weir
Neil
N
Catterall
Catherine
C
Baker
Terry S
TS
Jones
Malcolm N
MN
D017252:CD3 Complex / Q000096:biosynthesis; D005821:Genetic Techniques; D005822:Genetic Vectors; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019169:Jurkat Cells; D008081:Liposomes / Q000276:immunology* / Q000378:metabolism*; D008854:Microscopy, Electron; D019301:Oleic Acid / Q000494:pharmacology; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D013601:T-Lymphocytes / Q000378:metabolism; D013696:Temperature; D014162:Transfection; D001616:beta-Galactosidase / Q000378:metabolism
D017252:CD3 Complex; D008081:Liposomes; D010714:Phosphatidylethanolamines; C038089:1,2-distearoylphosphatidylethanolamine; D019301:Oleic Acid; D001616:beta-Galactosidase; D005947:Glucose