Early regulation of hypothalamic arcuate nucleus CART gene expression by short photoperiod in the Siberian hamster.
Cocaine- and amphetamine-regulated transcript (CART) mRNA is expressed in a number of hypothalamic nuclei including the arcuate nucleus (ARC). An increase in CART gene expression in the ARC of juvenile female Siberian hamsters (Phodopus sungorus) 14 days after transfer to short photoperiod at weaning and prior to major divergence of body weight trajectory in this seasonal mammal implicates CART in the induction of programmed weight change. In the current series of experiments, elevated CART mRNA in short photoperiod juvenile female animals relative to long photoperiod controls was apparent throughout the caudal-rostral extent of the ARC after 14 days, but was not observed when short photoperiod exposure was limited to 4-7 days. Elevated CART gene expression was also observed in juvenile males 14 days after transfer to short photoperiod at weaning, in adult female hamsters 14 days after transfer to short photoperiod and in adult male hamsters 21 days after transfer to short photoperiod. There were no consistent trends in expression levels of other energy balance-related genes with these relatively short duration photoperiod manipulations, suggesting that CART may be involved in short photoperiod-programmed body weight regulation.
Mercer
Julian G
JG
Molecular Neuroendocrinology Group, Aberdeen Centre for Energy Regulation and Obesity, Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, United Kingdom. jgm@rri.sari.ac.uk
Ellis
Claire
C
Moar
Kim M
KM
Logie
Tracy J
TJ
Morgan
Peter J
PJ
Adam
Clare L
CL
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism / Q000502:physiology*; D001835:Body Weight / Q000502:physiology; D006224:Cricetinae; D005260:Female; D005786:Gene Expression Regulation / Q000528:radiation effects*; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics*; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000235:genetics / Q000378:metabolism; D017440:Photoperiod; D011333:Pro-Opiomelanocortin / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D018013:Receptors, Neuropeptide / Q000235:genetics / Q000378:metabolism; D012737:Sex Factors; D014886:Weaning; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D012333:RNA, Messenger; D018013:Receptors, Neuropeptide; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein; D011333:Pro-Opiomelanocortin