title: Steroidal saponins from rhizomes of Tupistra wattii Hook. f.
abstract: Chemical examination of the fresh rhizomes of Tupistra wattii HOOK. f. led to the isolation of three new steroidal saponins, wattoside G (1), H (2), and I (3), together with one known steroidal saponin, (25S)-1beta,3beta,4beta-trihydroxyspirotan-5beta-yl-O-beta-D-glucopyranoside (4). The structures of 1-3 were established to be (25R)-1beta,2beta,3beta,5beta-tetrahydroxyspirostan-4beta-yl-O-beta-D-xylopyranoside (1), (24S,25S)-24-[(beta-D-glucopyranosyl)oxy]-1beta,2beta,3beta,4beta,5beta,7beta-hexahydroxyspirostan-6-one (2), and (24S,25S)-1beta,3beta-dihydroxy-5beta-spirostan-24-yl-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside (3) on the basis of detailed analyses of physical, chemical, and spectral data. The isolated compounds were evaluated for cytotoxic activity against the cancer cell line K562 in vitro.
authors.affiliation: Department of Natural Products Chemistry, Shenyang Pharmaceutical University, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Shen
authors.forename: San-Long
authors.initials: SL
authors.lastname: Wang
authors.forename: Xi-Kui
authors.initials: XK
authors.lastname: Liu
authors.forename: Chong-Ren
authors.initials: CR
authors.lastname: Yang
authors.forename: Bing
authors.initials: B
authors.lastname: Cai
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Yao
mesh_terms: D006801:Humans; D020014:K562 Cells / Q000187:drug effects; D019668:Liliaceae; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D027343:Rhizome; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D010936:Plant Extracts; D012503:Saponins; D013256:Steroids