title: Comparison of inter- and intra-chromosomal aberrations in blood samples exposed to different dose rates of gamma radiation.
abstract: Peripheral blood samples obtained from a normal healthy volunteer were exposed in vitro to gamma radiation with various doses at different dose rates of 1.0, 0.1 and 0.0014 Gy min(-1). The exposed samples were analysed for different chromosomal aberrations such as dicentrics (DC), centric rings (CR) and double-minutes (DM). The ratio of DC chromosomes (inter) to the total number of centric rings (CR) and double-minutes (DM) (CR + DM = intra) were analysed for all the three dose rates. The study showed that the frequency of inter-arm chromosomal aberrations was more then three times higher than that observed with intra-arm chromosomal aberrations in samples exposed at a dose rate of 1.0 and 0.1 Gy min (-1). However, the frequency of inter- and intra-arm chromosomal aberrations were almost same (ratio 1:1) in samples exposed at a dose rate of 0.0014 Gy min(-1). This paper discusses the usefulness of the ratio of inter- and intra-arm chromosome aberration in finding out whether the sample was exposed to high or low dose rate radiation.
authors.affiliation: Genetics Research Cell, Sri Ramachandra Medical College and Research Institute, Deemed University, Porur, Chennai - 600 116, Tamil Nadu, India. prabhu_bk@yahoo.com
authors.forename: B
authors.initials: B
authors.lastname: Karthikeya-Prabhu
authors.forename: P
authors.initials: P
authors.lastname: Venkatachalam
authors.forename: S F D
authors.initials: SF
authors.lastname: Paul
authors.forename: K
authors.initials: K
authors.lastname: Muthuvelu
authors.forename: M
authors.initials: M
authors.lastname: Balu-David
authors.forename: M N
authors.initials: MN
authors.lastname: Mohankumar
authors.forename: R K
authors.initials: RK
authors.lastname: Jeevanram
mesh_terms: D000328:Adult; D001769:Blood / Q000528:radiation effects; D002869:Chromosome Aberrations; D019457:Chromosome Breakage; D002877:Chromosomes, Human / Q000528:radiation effects* / Q000648:ultrastructure; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure