Cutting edge: molecular cloning of a killer cell Ig-like receptor in the mouse and rat.
We report the molecular cloning of a KIR3DL1 receptor in the mouse and the rat, between 37.4 and 45.4% identical with primate killer cell Ig-like receptors (KIRs/CD158). Both mouse and rat molecules contain a pair of immunoreceptor tyrosine-based inhibition motifs in their cytoplasmic regions, suggesting an inhibitory function. Southern blot analysis indicated a single KIR gene in the rat, whereas the mouse genome contains more than one KIR-related element. The rat Kir3dl1 locus was mapped to the leukocyte receptor gene complex on chromosome 1, whereas mouse Kir3dl1 was localized to the X chromosome. RT-PCR demonstrated that KIR3DL1 was selectively expressed by NK cells in both rat and mouse. An epitope-tagged expression construct of mouse KIR3DL1 transfected into 293T cells induced expression of a approximately 55-kDa protein. Our data indicate that KIR receptors may contribute to the NK cell receptor repertoire in rodents, alongside the Ly-49 family.
Hoelsbrekken
Sigurd E
SE
Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway. s.e.hoelsbrekken@studmed.uio.no
Nylenna
Øyvind
Ø
Saether
Per C
PC
Slettedal
Imer O
IO
Ryan
James C
JC
Fossum
Sigbjørn
S
Dissen
Erik
E
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000302:isolation & purification; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D051381:Rats; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D054340:Receptors, KIR; D054341:Receptors, KIR3DL1; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic / Q000276:immunology; D014162:Transfection
D018076:DNA, Complementary; C515083:KIR3DL1 protein, human; D008565:Membrane Proteins; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054341:Receptors, KIR3DL1