title: Quercetin suppresses proinflammatory cytokines production through MAP kinases andNF-kappaB pathway in lipopolysaccharide-stimulated macrophage.
abstract: Quercetin is a flavonoid molecule ubiquitous in nature and functions as an anti-oxidant and anti-inflammatory agent with little toxicity in vivo and in vitro. Dose- and time-dependent effect of quercetin has been investigated on proinflammatory cytokine expression and NO production, focusing on its effects on the MAP kinases and the NF-kappaB signal transduction pathways in LPS-stimulated RAW 264.7 cells by using RT-PCR and immunoblotting. Quercetin strongly reduced activation of phosphorylated ERK kinase and p38 MAP kinase but not JNK MAP kinase by LPS treatment. In addition, quercetin treatment inhibited NF-kappaB activation through stabilization of the NF-kappaB/IkappaB complex and IkappaB degradation and proinflammatory cytokines and NO/iNOS expression. Quercetin may exert its anti-inflammatory and immunomodulatory properties in the effect molecules such as proinflammatory cytokines and NO/iNOS by suppressing the activation of ERK and p38 MAP kinase, and NF-kappaB/IkappaB signal transduction pathways.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Yusung, Taejon City, South Korea.
authors.forename: Sung-Yeon
authors.initials: SY
authors.lastname: Cho
authors.forename: Sang-Joon
authors.initials: SJ
authors.lastname: Park
authors.forename: Myung-Ja
authors.initials: MJ
authors.lastname: Kwon
authors.forename: Tae-Sook
authors.initials: TS
authors.lastname: Jeong
authors.forename: Song-Hae
authors.initials: SH
authors.lastname: Bok
authors.forename: Woo-Young
authors.initials: WY
authors.lastname: Choi
authors.forename: Won-Il
authors.initials: WI
authors.lastname: Jeong
authors.forename: Si-Yun
authors.initials: SY
authors.lastname: Ryu
authors.forename: Sun-Hee
authors.initials: SH
authors.lastname: Do
authors.forename: Cha-Soo
authors.initials: CS
authors.lastname: Lee
authors.forename: Jae-Chan
authors.initials: JC
authors.lastname: Song
authors.forename: Kyu-Shik
authors.initials: KS
authors.lastname: Jeong
mesh_terms: D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D020672:I-kappa B Proteins / Q000378:metabolism; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013997:Time Factors; D048051:p38 Mitogen-Activated Protein Kinases
chemical_list: D016207:Cytokines; D020672:I-kappa B Proteins; D008070:Lipopolysaccharides; D016328:NF-kappa B; C000606205:Nfkbia protein, mouse; D012333:RNA, Messenger; D000072000:NF-KappaB Inhibitor alpha; D009569:Nitric Oxide; D011794:Quercetin; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases