The effect of extracellular calcium ion on gene expression of bone-related proteins in human pulp cells.
Calcium hydroxide is often used for induction of reparative dentin formation in endodontic treatment. However, little is known about the mechanism by which calcium hydroxide works. The calcium ion (Ca2+) is an important regulator of cell functions. In this study, we examined the effect of extracellular Ca2+ on gene expression of bone-related proteins in human cultured pulp cells in serum-free conditions. A Ca2+ level elevated by 0.7 mM induced an increase in mRNA expression of osteopontin and bone morphogenetic protein (BMP)-2. However, mRNA levels of BMP-4 and alkaline phosphatase decreased under the elevated Ca2+ culture condition. The same concentration of additional magnesium ions had little effect on expressions of the examined bone-related protein mRNAs. These findings suggest that Ca2+ in Ca(OH)2 specifically modulates osteopontin and BMP-2 levels during calcification in pulp.
Rashid
Fahmida
F
Department of Periodontology and Endodontology, Hiroshima University Faculty of Dentistry, Japan.
Shiba
Hideki
H
Mizuno
Noriyoshi
N
Mouri
Yoshihiro
Y
Fujita
Tsuyoshi
T
Shinohara
Hiroaki
H
Ogawa
Tetsuji
T
Kawaguchi
Hiroyuki
H
Kurihara
Hidemi
H
D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000235:genetics; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis* / Q000235:genetics; D002118:Calcium / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003809:Dentin, Secondary / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D053495:Osteopontin; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000096:biosynthesis* / Q000235:genetics; D016212:Transforming Growth Factor beta
C525718:BMP2 protein, human; C525809:BMP4 protein, human; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D012333:RNA, Messenger; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D016212:Transforming Growth Factor beta; D053495:Osteopontin; D000469:Alkaline Phosphatase; D008274:Magnesium; D002126:Calcium Hydroxide; D002118:Calcium