Biological and environmental factors affecting ultrasound-induced hemolysis in vitro: 2. Medium dissolved gas (pO2) content.
The data collected in this project supported the a priori hypothesis that the concentration of dissolved oxygen in whole human blood in vitro affected the extent of ultrasound (US)-induced hemolysis under conditions conducive to the occurrence of inertial cavitation. Aliquots of whole human blood in vitro with a relatively high O(2) level had statistically significantly more 1-MHz US-induced hemolysis than aliquots with a relatively low O(2) level in the presence of controlled gas nucleation (Albunex or ALX, supplementation), with US-induced hemolytic yields being substantially less at 2.2- and 3.5-MHz exposures or in the absence of ALX-supplementation at otherwise comparable acoustic pressures, pulse lengths and duty factors. Passive cavitation detection (pcd) measures indicated a linear relationship for hemolysis up to about 70% and pcd values (R(2) = 0.99).
Miller
Morton W
MW
Department of Obstetrics and Gynecology, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642-0001, USA. Morton_Miller@urmc.rochester.edu
Everbach
E Carr
EC
Miller
W Marcus
WM
Battaglia
Linda F
LF
D000418:Albumins / Q000494:pharmacology; D003287:Contrast Media / Q000494:pharmacology; D004912:Erythrocytes / Q000000981:diagnostic imaging; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D010100:Oxygen / Q000097:blood*; D011312:Pressure; D013696:Temperature; D014463:Ultrasonography
D000418:Albumins; C060687:Albunex; D003287:Contrast Media; D010100:Oxygen