Improved detection of higher molecular weight proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on polytetrafluoroethylene surfaces.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) of proteins was performed on a range of polytetrafluoroethylene (PTFE) surfaces. Sinapinic acid and alpha-cyano-4-hydroxycinnamic acid matrices were compared and the order of application varied to identify the best combination for each surface. It is demonstrated that the use of a PTFE surface improves the intensity of signals obtained for higher molecular weight proteins.
Botting
Catherine H
CH
Centre for Biomolecular Sciences, School of Chemistry, University of St Andrews, Fife KY16 9ST, Scotland, UK. cb2@st-andrews.ac.uk
D000818:Animals; D002417:Cattle; D003207:Conalbumin / Q000032:analysis / Q000737:chemistry; D003373:Coumaric Acids; D008970:Molecular Weight; D010763:Phosphorylase b / Q000032:analysis / Q000737:chemistry; D011138:Polytetrafluoroethylene; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D003373:Coumaric Acids; D011506:Proteins; D003207:Conalbumin; D012710:Serum Albumin, Bovine; C007175:alpha-cyano-4-hydroxycinnamate; C073734:sinapinic acid; D011138:Polytetrafluoroethylene; D010763:Phosphorylase b