Nevirapine resistance mutation at codon 181 of the HIV-1 reverse transcriptase confers stavudine resistance by increasing nucleotide substrate discrimination and phosphorolytic activity.
Recombinant HIV-1 reverse transcriptase (RT) carrying non-nucleoside inhibitors (NNRTIs) resistance mutation at codon 181 showed reduced incorporation and high efficiency of phosphorolytic removal of stavudine, a nucleoside RT inhibitor. These results reveal a new mechanism for cross-resistance between different classes of HIV-1 RT inhibitors.
Blanca
Giuseppina
G
Istituto di Genetica Molecolare, Consiglio Nazionale delle Ricerche, via Abbiategrasso 207, I-27100 Pavia, Italy.
Baldanti
Fausto
F
Paolucci
Stefania
S
Skoblov
Alexander Yu
AY
Victorova
Lyubov
L
Hübscher
Ulrich
U
Gerna
Giuseppe
G
Spadari
Silvio
S
Maga
Giovanni
G
D003062:Codon; D024882:Drug Resistance, Viral; D054303:HIV Reverse Transcriptase / Q000235:genetics*; D009154:Mutation; D019829:Nevirapine / Q000494:pharmacology*; D009711:Nucleotides / Q000378:metabolism; D010766:Phosphorylation; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology*; D018119:Stavudine / Q000494:pharmacology*; D015215:Zidovudine / Q000494:pharmacology
D003062:Codon; D009711:Nucleotides; D018894:Reverse Transcriptase Inhibitors; D015215:Zidovudine; D019829:Nevirapine; D018119:Stavudine; D054303:HIV Reverse Transcriptase