Rapid differentiation between short-chain-length and medium-chain-length polyhydroxyalkanoate-accumulating bacteria with spectrofluorometry.
An approach for rapid differentiation between short-chain-length (scl) and medium-chain-length (mcl) polyhydroxyalkanoate (PHA) producers was developed. Polyhydroxyalkanoate-accumulated bacterial cells stained with Nile red were suspended in water and subjected to fluorescence spectroscopy at a fixed excitation wavelength of 488 nm. The scl-PHA-accumulated bacteria revealed a maximum emission wavelength at 590 nm, and for mcl-PHA producers were seen at a wavelength of 575 nm. Combining Nile red staining and fluorescence spectroscopy, the accumulated PHA granules could be rapidly differentiated into scl-PHA and mcl-PHA from the intact cells.
Wu
Hsan-Au
HA
Department of Agricultural Chemistry, National Taiwan University, 1, Sec. 4, Roosevelt Rd., Taipei 106, Taiwan.
Sheu
Der-Shyan
DS
Lee
Chia-Yin
CY
D000217:Acyltransferases / Q000378:metabolism; D001419:Bacteria / Q000145:classification / Q000201:enzymology / Q000378:metabolism*; D002210:Caprylates / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry*; D005942:Gluconates / Q000378:metabolism; D010078:Oxazines / Q000737:chemistry; D011108:Polymers / Q000032:analysis / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*
D002210:Caprylates; D005227:Fatty Acids; D005942:Gluconates; D010078:Oxazines; D011108:Polymers; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase; C031492:octanoic acid; C044808:nile red; C030691:gluconic acid