title: RAPD-PCR and PFGE as tools in the investigation of an outbreak of beta-haemolytic Streptococcus group A in a Swedish hospital.
abstract: We evaluated the random amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) techniques for studying an outbreak of beta-haemolytic streptococci group A (GAS) occurred at two maternity wards at Danderyd hospital, Stockholm, Sweden. All the isolates were of T-type 8,25. The RAPD technique revealed that all RAPD-PCR profiles were identical. PFGE showed that all the patterns but one were identical. These patterns were compared with 10 different T-type GAS from the strain collection of the Swedish Institute for Infectious Disease Control (SMI) and T-type 8,25 from different years and locations. The SMI strains exhibited patterns different from each other and all different from the isolates from Danderyd hospital. Moreover, RAPD could not differentiate among the T-type 8,25 isolates from different years and locations but PFGE showed differences among the amplicons. Our results indicated that the RAPD and PFGE techniques could be efficient tools in epidemiological studies of GAS.
authors.affiliation: Section of Bacteriology, SLU, Box 7036, Klinikcentrum, Travv. IOC, 750 07 Uppsala, Sweden. carlos.gonzalez.rey@vmm.slu.se
authors.forename: C
authors.initials: C
authors.lastname: González-Rey
authors.forename: A M
authors.initials: AM
authors.lastname: Belin
authors.forename: H
authors.initials: H
authors.lastname: Jörbeck
authors.forename: M
authors.initials: M
authors.lastname: Norman
authors.forename: K
authors.initials: K
authors.lastname: Krovacek
authors.forename: B
authors.initials: B
authors.lastname: Henriques
authors.forename: G
authors.initials: G
authors.lastname: Källenius
authors.forename: S B
authors.initials: SB
authors.lastname: Svenson
mesh_terms: D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005260:Female; D006771:Hospitals, Maternity; D006801:Humans; D011247:Pregnancy; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013548:Sweden / Q000453:epidemiology
chemical_list: D004269:DNA, Bacterial