Further characterization of Chinese hamster carbonyl reductases (CHCRs).
Three cDNAs encoding carbonyl reductases (CHC1-3) have been isolated and expressed in bacterial cells. The recombinant enzymes were further characterized with respect to substrate specificity, inhibitor sensitivity and response to pyruvate. In addition, the expression of the mRNAs of CHCRs encoding in brain, liver and kidney was analyzed by RT-PCR. Searches of EST files revealed that orthologues of both CHCR3 and human CBR3 are expressed in variety of human cells and tissues.
Terada
Tomoyuki
T
Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, 565-0871, Osaka, Japan. terada@phs.osaka-u.ac.jp
Sugihara
Yukiko
Y
Nakamura
Kayo
K
Mizobuchi
Hidetoshi
H
Maeda
Masatomo
M
D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D006224:Cricetinae; D003412:Cricetulus; D004791:Enzyme Inhibitors / Q000494:pharmacology; D020224:Expressed Sequence Tags; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity
D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000429:Alcohol Oxidoreductases