Integrin expression patterns during early limb muscle development in the mouse.
Cell-extracellular matrix interactions play crucial roles in limb muscle development but practically nothing is known on what integrins are involved before the differentiation of muscle precursor cells (MPCs) in the limb muscle masses. In this study we determine the expression patterns of integrins during early forelimb muscle development in the mouse. alpha6beta1 integrin is downregulated in the lateral dermomyotome when delamination of MPCs occurs. In late E9.5 embryos, alpha1beta1 and alpha5beta1 are expressed in a pattern very similar to pax3, which marks MPCs migrating to the limb bud. After myf5 upregulation in the limb bud, alpha1beta1 and alpha5beta1 expression is maintained and the alpha4beta1 integrin starts being expressed.
Bajanca
Fernanda
F
Department of Zoology, Centre for Environmental Biology, Faculty of Sciences, University of Lisbon, 1749-016 Lisbon, Portugal.
Thorsteinsdóttir
Sólveig
S
D000818:Animals; D005121:Extremities / Q000196:embryology / Q000502:physiology; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D019012:Integrin beta1 / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice / Q000196:embryology; D018482:Muscle, Skeletal / Q000196:embryology / Q000378:metabolism*
D019012:Integrin beta1