[Study of IS-elements aimed at establishing a degree of the relationship of multiple-serotype Streptococcal strains of group B].
The presence and restriction fragment length polymorphism of IS 861 and IS 1548 were analyzed among 113 streptococcal strains of group B. 13 strains were found to possess both IS 861 and IS 1548, 54 strains were found to possess only IS 861, and 46 strains had neither IS 861 nor IS 1548. 9 HindIII and 6 EcoRI restriction patterns, hybridizing correspondingly with IS 861 and IS 1548, were discovered. A certain correlation between IS 1548 and IS 861 hybridizing patterns, on the one hand, and restriction patterns, found by pulse-electrophoresis, on the other hand, was established. It was demonstrated that restriction fragment length polymorphism of IS 1548 and 861 can be used to evaluate the degree of relationship between the strains.
Dmitriev
A V
AV
Ming
Y
Y
Suvorov
A N
AN
Yonghong
Y
Y
D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D012703:Serotyping; D013292:Streptococcus agalactiae / Q000145:classification* / Q000235:genetics*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial