[Interaction of human apolipoprotein AI and HIV-1 envelope proteins with the native and recombinant CD4 receptors].
The method of enzyme-linked immunosorbent assay (ELISA) was used to show an interaction of soluble recombinant CD4-receptor (rsCD4) with human apolipoprotein A-1. Competitive interactions between envelope proteins VIH-1 (gp120 and gp41), on the one hand, and human apolipoprotein A-1 with CD4 receptor, present in the cellular membranes of line MT4 human lymphocytes, were demonstrated by the method of flow cytofluorimetry. It was suggested that the competitive interactions between the above proteins could manifest in respect to the apolipoprotein A-1 receptor, which affects the involvement of the latter in the regulation of protein biosynthesis and which leads to a decrease in the body weight of HIV-infected patients.
Panin
L E
LE
Kostina
N E
NE
D016632:Apolipoprotein A-I / Q000737:chemistry / Q000378:metabolism*; D001667:Binding, Competitive; D015704:CD4 Antigens / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015699:HIV Envelope Protein gp120 / Q000378:metabolism; D015700:HIV Envelope Protein gp41 / Q000378:metabolism; D015497:HIV-1; D006801:Humans; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D018110:Receptors, Lipoprotein / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000378:metabolism*
D016632:Apolipoprotein A-I; D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D018110:Receptors, Lipoprotein; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins