title: Flow-cytometric analysis of T-lymphocyte subsets after different treatment methods in patients with pericoronitis.
abstract: OBJECTIVE
  The aim of this study was to determine whether there was any change in T-lymphocyte subsets in patients with periocoronitis after the application of different treatment methods.
  
  
  METHODS
  Twenty-six patients with acute pericoronitis were included in the study. In every phase of the treatment (pretreatment, postcurettage, and postextraction), the biopsy samples were taken from the gingival tissues at sites of pericoronitis. Then, CD4(+) and CD8(+) lymphocyte and CD4(+)/CD8(+) ratio values were determined using flow cytometry in the biopsy samples. At the same time, gingival index (Löe-Silness) and plaque index (Silness-Löe) scores were recorded to assess the periodontal status in patients. To determine the correlation between the clinical measurements and the laboratory results obtained before the treatment, after curettage, and after extraction, we conducted an analysis using a paired t-test.
  
  
  RESULTS
  The normal values in peripheral blood of CD4(+) and CD8(+) lymphocytes are 25% to 29% and 19% to 48%, respectively. However, the CD4(+) and CD8(+) lymphocyte values in the patients with acute pericoronitis were found to be 22.12% +/- 6.15% and 7.69% +/- 4.12%, respectively. These values are lower than the normal values. The CD4(+) lymphocyte value increased to 31.06% +/- 7.09% postcurettage and to 32.24% +/- 3.11% postextraction. The CD8(+) lymphocyte value increased to 16.21% +/- 5.27% postcurettage and to 18.25% +/- 3.13% postextraction. The CD4/CD8 ratio increased postcurettage and postextraction. This increase was statistically significant (P <.001). Postcurettage, there was decrease in clinical indexes, which was statistically significant (P <.001). A significant correlation between CD4(+) lymphocyte and ginigival index values and also between CD8(+) lymphocyte and plaque index values was determined postcurettage (P <.05).
  
  
  CONCLUSIONS
  CD4(+) and CD8(+) T-lymphocytes could play a significant role in pericoronitis pathobiology.
authors.affiliation: Department of Periodontology and Oral Surgery, Atatürk University, Faculty of Dentistry, Erzurum, Turkey. receporbak@yahoo.com
authors.forename: Recep
authors.initials: R
authors.lastname: Orbak
authors.forename: Ertunç
authors.initials: E
authors.lastname: Dayi
mesh_terms: D000328:Adult; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003774:Dental Plaque Index; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010497:Pericoronitis / Q000097:blood / Q000276:immunology* / Q000628:therapy*; D010512:Periodontal Index; D018709:Statistics, Nonparametric; D013357:Subgingival Curettage