title: [Express of human papillomavirus, P53 and H-ras gene in laryngeal cancer]
abstract: OBJECTIVE: To study the abnormality of p53 and H-ras gene in laryngeal cancer cells and their relation to the infection of human papillomavirus(HPV) subtypes. METHODS: The HPV subtypes, P53 and H-ras gene were examined in 28 cases of laryngeal cancer by polymerase chain reaction (PCR), single strand conformation polymorphism(SSCP), restriction fragment length polymorphism (RFLP) and DNA sequencing. RESULTS: P53 gene mutation existed in 50%cases of laryngeal cancer and DNA sequencing showed base substitution, insertion or deletion in 4 cases; The positive rate of the H-ras oncogene mutation was only 3.57% All high risk HPV infections (HPV-16 or-18) were distinctly seen in the cancer cases, especially in cases with P53 mutation. CONCLUSION: The high risk HPV subtype infection and P53 gene abnormality may play an important role in laryngeal cancer progression and these two factors may be correlated to each other.
authors.affiliation: The First Affiliated Hospital, College of Medical Sciences, Zhejiang University, Hangzhou 310003, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Lin
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Chen
authors.forename: Hui-Ming
authors.initials: HM
authors.lastname: Wang