Phasing at high resolution using Ta6Br12 cluster.
The Ta(6)Br(12)(2+) cluster compound is known to be a powerful reagent for derivatization of crystals of large macromolecules at low resolution. The cluster is a regular octahedron of six Ta atoms with 12 bridging Br atoms at the edges of the octahedron. The cluster is compact, of approximately spherical shape, with a radius of about 6 A. Both tantalum and bromine display a significant anomalous diffraction signal at their absorption edges at 1.25 and 0.92 A, respectively. At resolutions lower than 5 A the tantalum cluster behaves as a super-atom and provides very large isomorphous and anomalous signals, which significantly diminish at about 4 A. However, beyond 3 A the individual Ta atoms can be resolved and the phasing power of the cluster increases again. The Ta(6)Br(12)(2+) cluster has been used for phasing four different proteins at high resolution. Ta(6)Br(12)(2+) appeared to be a mild derivatization reagent and, despite partial incorporation, led to a successful solution of crystal structures by the single-wavelength anomalous diffraction (SAD) approach.
Banumathi
Sankaran
S
Synchrotron Radiation Research Section, Macromolecular Crystallography Laboratory, NCI, Brookhaven National Laboratory, Building 725A-X9, Upton, NY 11973, USA.
Dauter
Miroslawa
M
Dauter
Zbigniew
Z
D001965:Bromides / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D007202:Indicators and Reagents; D008958:Models, Molecular; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D013635:Tantalum / Q000737:chemistry*
D001965:Bromides; D007202:Indicators and Reagents; D011506:Proteins; C107153:tantalum bromide; D013635:Tantalum