Evaluation of warm ischemia-reperfusion injury using heat shock protein in the rat liver.
We focused on heat shock protein 70 (HSP70) as a marker of viability in hepatic warm ischemia-reperfusion. Segmental hepatic warm ischemia was produced in rats for 15, 30, 60, 90, 120, or 180 min. Liver sections were evaluated at 30, 60, and 120 min of reperfusion. Expression of HSP70 and messenger RNA (mRNA), apoptosis, and apoptosis-associated genes such as Bcl-2 and Bax were studied. Expression of HSP70 and mRNA was augmented as warm ischemia was prolonged, but was markedly suppressed in livers with more than 120 min of ischemia. The highest accumulation of HSP70 was observed in the nucleus. In livers subjected to longer duration of warm ischemia, necrosis and apoptosis were evident and Bcl-2 mRNA expression and Bcl-2/Bax protein ratio were markedly diminished. Apoptosis may be related to the process of cellular injury induced by warm ischemia-reperfusion. Expression of HSP70 and the Bcl-2 family can be effective markers of viability in hepatic warm ischemia-reperfusion.
Sakai
Takehiro
T
The First Department of Surgery, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki, 036-8562 Japan. tsakai@cc.hirosaki-u.ac.jp
Takaya
Shunichi
S
Fukuda
Akinari
A
Harada
Osamu
O
Kobayashi
Makoto
M
D000818:Animals; D017209:Apoptosis; D015415:Biomarkers; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000235:genetics*; D006416:Hematoxylin; D006358:Hot Temperature; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008099:Liver / Q000737:chemistry / Q000473:pathology*; D016031:Liver Transplantation; D008297:Male; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology*; D013194:Staining and Labeling; D051028:bcl-2-Associated X Protein
C494828:Bax protein, rat; D015415:Biomarkers; D004396:Coloring Agents; D018840:HSP70 Heat-Shock Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin