Evidence for glycosylphosphatidylinositol (GPI)-anchored eosinophil-derived neurotoxin (EDN) on human granulocytes.
Eosinophil-derived neurotoxin (EDN) is one of the four basic proteins stored in specific eosinophil granules. Here we demonstrate that EDN can also be detected at the surface of granulocytes. Reduction of EDN membrane expression after phosphatidylinositol-specific phospholipase C treatment suggests that a glycosylphosphatidylinositol (GPI) anchor is involved in the membrane association of EDN. The presence of a GPI anchor was confirmed by a lower expression of membrane EDN on granulocytes from patients with paroxysmal nocturnal hemoglobinuria which present cells lacking GPI anchor proteins. Furthermore, metabolic labeling with GPI anchor components supports biochemical evidence of GPI anchoring of EDN.
Debierre-Grockiego
Françoise
F
Institute for Virology, Medical Center for Hygiene, Philipps University, D-35037 Marburg, Germany. debierre@mailer.uni-marburg.de
Desaint
Corinne
C
Fuentes
Vincent
V
Poussin
Mathilde
M
Socié
Gérard
G
Azzouz
Nahid
N
Schwarz
Ralph T
RT
Prin
Lionel
L
Gouilleux-Gruart
Valérie
V
D001782:Blood Donors; D003594:Cytoplasmic Granules / Q000737:chemistry; D047208:Eosinophil-Derived Neurotoxin; D004802:Eosinophilia / Q000097:blood; D004804:Eosinophils / Q000737:chemistry; D005434:Flow Cytometry; D017261:Glycosylphosphatidylinositols / Q000502:physiology*; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood; D006801:Humans; D007958:Leukocyte Count; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D012016:Reference Values; D012260:Ribonucleases / Q000097:blood / Q000633:toxicity*
D017261:Glycosylphosphatidylinositols; D047208:Eosinophil-Derived Neurotoxin; D012260:Ribonucleases