title: [Short time bacterial endotoxins test for positron emission tomography by means of positively charged filters].
abstract: Positron emission tomography (PET) radiotracers have very short physical half-lives. It is hard to complete a bacterial endotoxins test prior to release from medical institutes. For endotoxin quantitative determination, limulus amebocyte lysate (LAL) reagent and kinetic-turbidimetry system were previously developed. We investigated the possibility of a short time test by means of positively charged filters. As a result of this study, the effects of positively charged filters on endotoin removal were over 99.5% for [18F]FDG and [18F]NaF, which were contaminated with the indicated concentration of endotoxin. Combining this filter and the kinetic-turbidimetric method, it was possible to complete a bacterial endotoxins test in 5 min prior to the patient's administration. This test should be required prior to release for PET radiopharmaceutical quality control. It has been suggested that this combination is a good method for this purpose.
authors.affiliation: Nishijin Hospital.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Nakazawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Wakita
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Mineura
authors.forename: Masaru
authors.initials: M
authors.lastname: Nakamura
authors.forename: Ryou
authors.initials: R
authors.lastname: Fujii
authors.forename: Hirotomo
authors.initials: H
authors.lastname: Nakanishi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Uji
authors.forename: Shirou
authors.initials: S
authors.lastname: Matsuura
authors.forename: Kenji
authors.initials: K
authors.lastname: Itani
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kanatsuna
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Ido
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Imahori
mesh_terms: D004340:Drug Contamination / Q000517:prevention & control*; D004731:Endotoxins / Q000032:analysis / Q000302:isolation & purification*; D019788:Fluorodeoxyglucose F18; D006863:Hydrogen-Ion Concentration; D008851:Micropore Filters; D009391:Nephelometry and Turbidimetry; D011786:Quality Control; D019275:Radiopharmaceuticals; D012969:Sodium Fluoride; D014055:Tomography, Emission-Computed
chemical_list: D004731:Endotoxins; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; D012969:Sodium Fluoride