Decreased expression of catalase mRNA in thyroid anaplastic carcinoma.
BACKGROUND
A decreased expression of glutathione peroxidase mRNA, an antioxidant enzyme, was previously observed in thyroid anaplastic carcinomas.


METHODS
To clarify the expression of antioxidant-related enzymes in thyroid anaplastic carcinomas, the expression levels of catalase, copper and zinc superoxide dismutase and manganese superoxide dismutase mRNA in 85 benign and malignant thyroid tissues were measured by means of real-time quantitative reverse transcription-polymerase chain reaction.


RESULTS
Decreased expression levels of catalase and copper and zinc superoxide dismutase mRNAs, but not manganese mRNA, were observed in five anaplastic carcinomas compared with normal thyroid tissues and differentiated tumors.


CONCLUSIONS
These results suggest the possibility that anaplastic carcinoma cells are more likely to suffer damage by oxygen free radicals than normal thyroid cells or differentiated tumor cells.
Hasegawa
Yukiko
Y
Department of Laboratory Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
Takano
Toru
T
Miyauchi
Akira
A
Matsuzuka
Fumio
F
Yoshida
Hiroshi
H
Kuma
Kanji
K
Amino
Nobuyuki
N
D000236:Adenoma / Q000378:metabolism; D002277:Carcinoma / Q000201:enzymology* / Q000378:metabolism; D002291:Carcinoma, Papillary / Q000378:metabolism; D002374:Catalase / Q000096:biosynthesis* / Q000235:genetics; D003300:Copper / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000201:enzymology* / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
D012333:RNA, Messenger; D003300:Copper; D002374:Catalase; D013482:Superoxide Dismutase; D015032:Zinc