title: [Identification of orthopoxvirus species using oligonucleotide micro-chips].
abstract: A method for describing the Orthopoxviruses that are pathogenic both to man and animals is described in the article. The method is based on hybridization of a fluorescently labelled amplified DNA sample with oligonucleotides, which were immobilized in a microchip. Species-specific regions within the crmB gene encoding a viral analogue of the tumor necrosis factor receptor, i.e. an important gene determining the pathogenicity of the mentioned Orthopoxviruses type, were used as a target for identification. The identification procedure takes around 6 hours and does not demand any costly equipment (a portable fluorescent microscope can be used).
authors.forename: M V
authors.initials: MV
authors.lastname: Mikheev
authors.forename: S A
authors.initials: SA
authors.lastname: Lapa
authors.forename: S N
authors.initials: SN
authors.lastname: Shchelkunov
authors.forename: A K
authors.initials: AK
authors.lastname: Chikova
authors.forename: V M
authors.initials: VM
authors.lastname: Mikhaĭlovich
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Sobolev
authors.forename: I V
authors.initials: IV
authors.lastname: Babkin
authors.forename: D A
authors.initials: DA
authors.lastname: Griadunov
authors.forename: M A
authors.initials: MA
authors.lastname: Bulavkina
authors.forename: A A
authors.initials: AA
authors.lastname: Gus'kov
authors.forename: E B
authors.initials: EB
authors.lastname: Sokunova
authors.forename: G V
authors.initials: GV
authors.lastname: Kochneva
authors.forename: V M
authors.initials: VM
authors.lastname: Blinov
authors.forename: L S
authors.initials: LS
authors.lastname: Sandakhchiev
authors.forename: A S
authors.initials: AS
authors.lastname: Zasedatelev
authors.forename: A D
authors.initials: AD
authors.lastname: Mirzabekov
mesh_terms: D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D018155:Orthopoxvirus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics*; D016415:Sequence Alignment; D013045:Species Specificity; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D015345:Oligonucleotide Probes; D018124:Receptors, Tumor Necrosis Factor; D014764:Viral Proteins; C089458:crmB protein, Orthopoxvirus