Protection against fatal Pseudomonas aeruginosa pneumonia in mice after nasal immunization with a live, attenuated aroA deletion mutant.
Studies of immunity to Pseudomonas aeruginosa have indicated that a variety of potential immunogens can elicit protection in animal models, utilizing both antibody- and cell-mediated immune effectors for protection. To attempt to optimize delivery of multiple protective antigens and elicit a broad range of immune effectors, we produced an aroA deletion mutant of the P. aeruginosa serogroup O2/O5 strain PAO1, designated PAO1deltaaroA. Previously, we reported that this strain elicits high levels of opsonic antibody directed against many serogroup O2/O5 strains after nasal immunization of mice and rabbits. Here, we assessed the protective efficacy of immunization with PAO1deltaaroA against acute fatal pneumonia in mice. After active immunization, high levels of protection were achieved against an ExoU-expressing cytotoxic variant of the parental strain PAO1 at doses up to 1,000-fold greater than the 50% lethal dose. Significant protection against PAO1 and two of four other serogroup O2/O5 strains was also found, but there was no protection against serogroup-heterologous strains. The serogroup O2/O5 strains not protected against were killed in opsonophagocytic assays as efficiently as the strains with which protection was seen, indicating a lack of correlation of protection and opsonic killing within the serogroup. In passive immunization experiments using challenge with wild-type PAO1 or other noncytotoxic members of the O2/O5 serogroup, there was no protection despite the presence of high levels of opsonic antibody in the mouse sera. However, passive immunization did prevent mortality from pneumonia due to the cytotoxic PAO1 variant at low-challenge doses. These data suggest that a combination of humoral and cellular immunity is required for protection against P. aeruginosa lung infections, that such immunity can be elicited by using aroA deletion mutants, and that a multivalent P. aeruginosa vaccine composed of aroA deletion mutants of multiple serogroups holds significant promise.
Priebe
Gregory P
GP
Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA. gpriebe@rics.bwh.harvard.edu
Meluleni
Gloria J
GJ
Coleman
Fadie T
FT
Goldberg
Joanna B
JB
Pier
Gerald B
GB
D000281:Administration, Intranasal; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D005260:Female; D007114:Immunization; D007928:Lethal Dose 50; D008070:Lipopolysaccharides / Q000276:immunology; D008168:Lung / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D019081:O Antigens / Q000235:genetics / Q000502:physiology*; D018410:Pneumonia, Bacterial / Q000517:prevention & control*; D011552:Pseudomonas Infections / Q000517:prevention & control*; D011817:Rabbits; D014613:Vaccines, Attenuated / Q000276:immunology
D001428:Bacterial Vaccines; D008070:Lipopolysaccharides; D019081:O Antigens; C095976:O antigen, Pseudomonas; D014613:Vaccines, Attenuated