title: Novel sample preparation method for safe and rapid detection of Bacillus anthracis spores in environmental powders and nasal swabs.
abstract: Bacillus anthracis spores have been used as a biological weapon in the United States. We wanted to develop a safe, rapid method of sample preparation that provided safe DNA for the detection of spores in environmental and clinical specimens. Our method reproducibly detects B. anthracis in samples containing <10 spores.
authors.affiliation: Center for Biological Defense, Department of Environmental and Occupational Health, College of Public Health, University of South Florida, Tampa, Florida 33612, USA. vluna@bt.suf.edu
authors.forename: Vicki A
authors.initials: VA
authors.lastname: Luna
authors.forename: Debra
authors.initials: D
authors.lastname: King
authors.forename: Carisa
authors.initials: C
authors.lastname: Davis
authors.forename: Tony
authors.initials: T
authors.lastname: Rycerz
authors.forename: Matthew
authors.initials: M
authors.lastname: Ewert
authors.forename: Andrew
authors.initials: A
authors.lastname: Cannons
authors.forename: Philip
authors.initials: P
authors.lastname: Amuso
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Cattani
mesh_terms: D001408:Bacillus anthracis / Q000302:isolation & purification*; D023482:Bioterrorism; D006801:Humans; D009666:Nose / Q000382:microbiology*; D011208:Powders; D013171:Spores, Bacterial / Q000302:isolation & purification*
chemical_list: D011208:Powders