title: Regulation of ipsilateral and contralateral bovine oviduct epithelial cell function in the postovulation period: a transcriptomics approach.
abstract: We studied differential gene expression in ipsilateral and contralateral bovine oviduct epithelial cells using a combination of subtracted cDNA libraries and cDNA array hybridization. Four Simmental heifers were synchronized and slaughtered 3.5 days after they entered standing heat. Epithelial cells were isolated from ipsilateral and contralateral oviducts. To identify genes that are differentially regulated in ipsilateral and contralateral epithelium, subtracted cDNA libraries were produced by suppression subtractive hybridization and analyzed by cDNA array hybridization. Sequencing of cDNAs showing differential expression levels in ipsilateral and contralateral epithelium revealed 35 different cDNAs, 30 of which matched genes with known functions and 5 of which matched genes without a known function. The majority of genes (n = 27) were expressed at a higher level in the ipsilateral oviduct, but for some genes (n = 8), mRNA abundance was higher in the contralateral oviduct. The regulated genes or their products include a variety of functional classes such as cell-surface proteins, cell-cell interaction proteins, members of signal transduction pathways, immune-related proteins, and enzymes. Identification of genes differentially regulated in ipsilateral and contralateral oviduct epithelial cells is the first step toward a systematic analysis of local mechanisms that regulate the function of the bovine oviduct epithelium in the postovulation period.
authors.affiliation: Institute of Molecular Animal Breeding, Ludwig Maximilians University, 81377 Munich, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Bauersachs
authors.forename: Helmut
authors.initials: H
authors.lastname: Blum
authors.forename: Sylvia
authors.initials: S
authors.lastname: Mallok
authors.forename: Hendrik
authors.initials: H
authors.lastname: Wenigerkind
authors.forename: Stephanie
authors.initials: S
authors.lastname: Rief
authors.forename: Katja
authors.initials: K
authors.lastname: Prelle
authors.forename: Eckhard
authors.initials: E
authors.lastname: Wolf
mesh_terms: D000818:Animals; D002417:Cattle; D004847:Epithelial Cells / Q000502:physiology; D005187:Fallopian Tubes / Q000166:cytology / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008183:Luteal Phase; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D014018:Tissue Distribution; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger