pcDNAL1 genetic immunization can induce specific cell-mediated immune responses in C57BL/6 mice.
OBJECTIVE
To investigate the specific cell-mediated immune efficacy of the an HPV16 prophylactic vaccine.


METHODS
C57BL/6 mice were randomly divided into 3 groups: experimental group I (treated with pcDNA L1), control group II (treated with pcDNA3.1) and control group III (treated with PBS buffer). The mice were immunized three times during a three-week interval. Ten to fourteen days after the third inoculation, a footpad swelling test was used to detect delayed-type hypersensitivity (DTH) responses. Antigen-specific splenocyte proliferation assay and quantitation of IFN-gamma cells in splenocytes were performed by FACS assay.


RESULTS
In the experimental group, splenocytes actively proliferated after stimulation with HPV16 VLP, and had developed a markedly larger amount of CD8(+) IFN-gamma(+) cells, which is an index for special CTL. Also, the footpad was significantly thickened upon inoculation with HPV16 VLP.


CONCLUSIONS
Naked DNA vaccine of HPV16 L1 can induce specific cell-mediated immune responses in mice, which should be considered for evaluation of HPV16 DNA vaccine feasibility.
Song
Jianming
J
Pathology laboratory, the Second Hospital of Shenzhen, Shenzhen 518035, China.
Sun
Xiangle
X
Liu
Tianju
T
Wang
Yili
Y
Si
Lüsheng
L
D000818:Animals; D006968:Hypersensitivity, Delayed / Q000209:etiology; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D027383:Papillomaviridae / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D019444:Vaccines, DNA / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D019444:Vaccines, DNA; D014765:Viral Vaccines; D007371:Interferon-gamma