Cloning and characterization of HEP21, a new member of the uPAR/Ly6 protein superfamily predominantly expressed in hen egg white.
Using two-dimensional (2D)-PAGE, partial protein internal sequencing, and PCR with degenerate primers, we cloned a novel cDNA named HEP21 from hen egg white. The 0.5-kb cDNA encodes a 106 amino acid protein with a cysteine spacing pattern suggesting that HEP21 is a new member of the uPAR/CD59/Ly-6/ snake neurotoxin superfamily. The closest homology of HEP21 is to mouse Ly-6C. Unlike most members of this protein family, HEP21 is not glycosylphosphatidylinositol (GPI)-anchored but is a secreted protein, as indicated by its localization and the presence of a signal peptide in its sequence. Moreover, HEP21 appears as an original member of this protein superfamily because it is predominantly expressed in a tissue, i.e., the oviduct, and especially the magnum where the egg white components are secreted.
Nau
F
F
UMR INRA-ENSAR 1055 Physico-chimie et Technologie des Ovoproduits, Pole d'Enseignement Supérieur et de Recherche Agronomique de Rennes, 65 rue de Saint-Brieuc CS 84215-35042 Rennes cedex, France. nau@agrorennes.educagri.fr
Guérin-Dubiard
C
C
Désert
C
C
Gautron
J
J
Bouton
S
S
Gribonval
J
J
Lagarrigue
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D004527:Egg Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D004529:Egg White / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015870:Gene Expression; D008969:Molecular Sequence Data; D010057:Oviducts / Q000737:chemistry; D019105:Random Amplified Polymorphic DNA Technique; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020539:Sequence Analysis, Protein; D017385:Sequence Homology
D018076:DNA, Complementary; D004527:Egg Proteins