Interaction of the anaphase-promoting complex/cyclosome and proteasome protein complexes with multiubiquitin chain-binding proteins.
Fission yeast Rhp23 and Pus1 represent two families of multiubiquitin chain-binding proteins that associate with the proteasome. We show that both proteins bind to different regions of the proteasome subunit Mts4. The binding site for Pus1 was mapped to a cluster of repetitive sequences also found in the proteasome subunit SpRpn2 and the anaphase-promoting complex/cyclosome (APC/C) subunit Cut4. The putative role of Pus1 as a factor involved in allocation of ubiquitinylated substrates for the proteasome is discussed.
Seeger
Michael
M
Medical Research Council Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, United Kingdom.
Hartmann-Petersen
Rasmus
R
Wilkinson
Caroline R M
CR
Wallace
Mairi
M
Samejima
Itaru
I
Taylor
Martin S
MS
Gordon
Colin
C
D000595:Amino Acid Sequence; D064173:Anaphase-Promoting Complex-Cyclosome; D002352:Carrier Proteins / Q000378:metabolism*; D003546:Cysteine Endopeptidases / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D006836:Hydro-Lyases / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D008025:Ligases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D011485:Protein Binding; D012568:Schizosaccharomyces / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D016415:Sequence Alignment; D043743:Ubiquitin-Protein Ligase Complexes
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; C108670:MTS4 protein, S pombe; D009097:Multienzyme Complexes; C490845:RHP23 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; D006836:Hydro-Lyases; C022479:pseudouridylate synthetase; D008025:Ligases