LOH of chromosome 12p correlates with Kras2 mutation in non-small cell lung cancer.
Previous observation has shown that the wild-type Kras2 allele is a suppressor of lung cancer in mice. Here we report that loss of heterozygosity (LOH) of chromosome 12p was detected in approximately 50% of human lung adenocarcinomas and large cell carcinomas, and Kras2 mutations were detected at codon 12 in approximately 40% of adenocarcinomas and large cell carcinomas. Interestingly, all of the lung adenocarcinomas and large cell carcinomas containing a Kras2 mutation exhibited allelic loss of the wild-type Kras2 allele when a correlation between LOH of the region on chromosome 12p and Kras2 mutation was made. These results from human lung cancer tissues provide a strong evidence in support of our previous observation in mouse models that the wild-type Kras2 is a tumor suppressor of lung cancer.
Li
Jie
J
Division of Human Cancer genetics, The Ohio Stsate University Comprehensive Cancer Center, 420 West 12th Avenue, Columbus, OH 43210, USA.
Zhang
Zhongqiu
Z
Dai
Zunyan
Z
Plass
Christoph
C
Morrison
Carl
C
Wang
Yian
Y
Wiest
Jonathan S
JS
Anderson
Marshall W
MW
You
Ming
M
D000230:Adenocarcinoma / Q000235:genetics*; D000483:Alleles; D018287:Carcinoma, Large Cell / Q000235:genetics*; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D005838:Genotype; D006801:Humans; D019656:Loss of Heterozygosity; D008175:Lung Neoplasms / Q000235:genetics*; D009363:Neoplasm Proteins / Q000172:deficiency / Q000235:genetics* / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000172:deficiency / Q000235:genetics* / Q000502:physiology; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins
C117307:KRAS protein, human; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins