The influence of fluoride on the cellular morphology and synthetic activity of the rat dentine-pulp complex in vitro.
Exposure to high fluoride concentrations in the immediate environment of the tissue is recognized to result in the post-translational modification of non-collagenous dentine extracellular matrix (ECM) components, potentially altering dentine mineralization. However, less is known about the effects of fluoride exposure on the morphology or metabolism of the cells associated with the dentine-pulp complex. This study examined the effects of fluoride exposure at defined concentrations on the cellular morphology and ECM synthetic activities of odontoblasts and pulpal fibroblasts by the culture of tooth sections from male Wistar rat incisors in Trowel-type cultures for up to 14 days, in the presence and absence of 6mM sodium fluoride. Histomorphometric analysis of the dentine-pulp complex of sodium fluoride-exposed tooth sections demonstrated no obvious gross morphological differences with respect to the odontoblasts and pulpal fibroblasts throughout the 14-day culture period, in comparison with unexposed tooth sections. No significant differences in odontoblast and pulpal fibroblast cell numbers were determined in the absence and presence of fluoride. Image analysis examination of odontoblast cytoplasmic:nuclear (C/N) ratios also showed no significant differences in fluoride-exposed and unexposed tooth sections, although reductions in the C/N ratios of pulpal fibroblasts were evident in fluoride-exposed sections at days 10 and 14. No significant differences in predentine width were observed in fluoride-exposed and unexposed tooth sections over the 14-day culture period. Autoradiography following [3H]proline incorporation into the dentine-pulp complex demonstrated inhibition of collagen synthesis, particularly by the odontoblasts in tooth sections exposed to 6mM sodium fluoride. These findings, in association with those from previous studies, imply that dentine ECM alterations may contribute to the altered mineralization of dentine during fluorosis, rather than secretory-related changes in odontoblast morphology.
Moseley
R
R
Department of Dental Health and Biological Sciences, Dental School, University of Wales College of Medicine, Heath Park, CF14 4XY, Cardiff, UK.
Sloan
A J
AJ
Waddington
R J
RJ
Smith
A J
AJ
Hall
R C
RC
Embery
G
G
D000818:Animals; D002327:Cariostatic Agents / Q000494:pharmacology*; D002452:Cell Count; D002467:Cell Nucleus / Q000648:ultrastructure; D003094:Collagen / Q000096:biosynthesis; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D003804:Dentin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted; D008297:Male; D009804:Odontoblasts / Q000187:drug effects / Q000648:ultrastructure; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar; D012969:Sodium Fluoride / Q000494:pharmacology*
D002327:Cariostatic Agents; D012969:Sodium Fluoride; D003094:Collagen