Comparison of tissue distribution and metabolism of 1,2- and 1,4-dibromobenzenes in female rats.
The distribution, excretion and metabolism of 1,4-dibromobenzene (1,4-DBB) and 1,2-dibromobenzene (1,2-DBB), following a single intraperitoneal administration to female Wistar rats, were investigated using radiotracer 3H and GC-MS technique. The maximum level of 3H after 1,4-DBB administration was detected in all examined rat tissues between 4 and 24 h foltowing the injection. The highest concentrations of 3H were found in fat tissue, muscles, adrenal glands and sciatic nerve. About 50% of administered dose was still retained in the rat 72 h after injection. For 1,2-DBB, the highest level of 3H was in the liver, kidneys and fat tissue 4 and 8 h after administration. Three days after injection, less than 2% of the given dose was retained in the rat body. Urine turned out to be the main route of 3H excretion following the injection of both compounds (30% and 82%, after 1,4-DBB and 1,2-DBB, respectively), and about 4% of the given dose was excreted in feces. In urine of rats the following substances were identified (in sequence 1,4-dBB and 1,2-dBB): (1) unchanged parent compounds (5 and 11%); (2) dibromophenols (84 and 73%); (3) dibromothiophenols (5 and 10%) and (4) monobromophenols (1.9 and 0.7%). This study suggests that 1,2-DBB is characterized by a relatively high turnover rate, whereas 1,4-DBB shows a tendency for long-term retention in the body.
Szymańska
Jadwiga A
JA
Department of Toxicology, Medical University, Lódź, Poland. jszymanska@pharm.am.lodz.pl
Sapota
Andrzej
A
Wesołowski
Wojciech
W
Czerski
Bogdan
B
Piotrowski
Jerzy K
JK
D000818:Animals; D001969:Bromobenzenes / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D002849:Chromatography, Gas; D005260:Female; D011044:Poland; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
D001969:Bromobenzenes; C102879:1,2-dibromobenzene; C066656:1,4-dibromobenzene