Establishment of in vivo metastasis model of human adenoid cystic carcinoma: detection of metastasis by PCR with human beta-globin gene.
Cells from the human adenoid cystic carcinoma (ACC) line, KOA-1, originating from the minor salivary gland and showing histological features of a dominantly solid pattern of ACC, were transplanted into the subcutaneous tissue of nude mice. The occurrence of lung metastasis was then examined both histologically and by PCR with a human-specific base arrangement of the beta-globin gene. Histologic examinations revealed lung metastasis in 5 of 30 mice. On the other hand, PCR showed a higher positive rate: 26 of 67 mice (0/10 at 1 month, 5/15 at 2 months, and 21/42 at 3 months after the transplantation). When some lungs which showed positive results by PCR analysis were transplanted into other mice, KOA-1 tumors developed. The results suggested that this experimental model could be a standard in vivo model of metastasis of human ACC.
Komatsubara
Hideki
H
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. hide@med.kobe-u.ac.jp
Umeda
Masahiro
M
Oku
Naohisa
N
Komori
Takahide
T
D000818:Animals; D001483:Base Sequence; D001707:Biopsy, Needle; D003528:Carcinoma, Adenoid Cystic / Q000473:pathology* / Q000556:secondary*; D004195:Disease Models, Animal; D005914:Globins / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness / Q000473:pathology; D009368:Neoplasm Transplantation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012468:Salivary Gland Neoplasms / Q000473:pathology*; D012680:Sensitivity and Specificity; D014183:Transplantation, Heterologous
D005914:Globins