Changes in free polyamines and related enzymes during stipule and pod wall development in Pisum sativum.
Level of free polyamines, their key metabolic enzymes, and other features related to ageing were examined during stipule and pod wall development in pea (Pisum sativum). Free polyamine titre (per unit fresh mass) in both the organs, the specific activities of arginine decarboxylase and ornithine decarboxylase in the pod wall, gradually decreased with maturation. In stipule, these enzymes attained peak activity at 15 days after pod emergence and declined thereafter. Ornithine decarboxylase activity was greater in pod wall than in stipule; while, arginine decarboxylase activity was higher in stipule. Activity of degradative enzyme diamine oxidase increased with the onset of senescence in both the organs. Chlorophyll and electrical conductance had a inverse relationship throughout the experimental period, whereas, the chlorophyll content was directly related with polyamine levels in both stipule and pod wall during aging. On the other hand, protein and RNA contents were positively correlated with free polyamines throughout the test period in stipule, but in the pod wall this was true only for the later stages of development.
Chattopadhyay
Soumen
S
Plant Physiology Laboratory, Department of Botany, Bose Institute, Calcutta 700 009, India. s_chatto25@hotmail.com
Lahiri
Kajari
K
Bharati
Ghosh
G
D000375:Aging / Q000502:physiology; D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D018744:DNA, Plant / Q000378:metabolism; D004553:Electric Conductivity; D018507:Gene Expression Regulation, Developmental; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D018532:Pisum sativum / Q000201:enzymology*; D010940:Plant Proteins / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism
D018744:DNA, Plant; D010940:Plant Proteins; D011073:Polyamines; D018749:RNA, Plant; D002734:Chlorophyll; D013096:Spermine; D006631:Amine Oxidase (Copper-Containing); D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; C019722:arginine decarboxylase; D013095:Spermidine; D011700:Putrescine