Determination of lead, cadmium and mercury in microwave-digested foodstuffs by RP-HPLC with an on-line enrichment technique.
A new method for the simultaneous determination of lead, cadmium and mercury ions in microwave-digested foodstuffs by reversed-phase high-performance liquid chromatography combined with on-line enrichment technique has been developed. The foodstuff samples were digested by microwave digestion. The lead, cadmium and mercury ions can be precolumn derivatized with 5,10,15,20-tetrakis(3-aminophenyl)porphine (T3APP) to form color chelates; then, the Hg-T3APP, Cd-T3APP and Pb-T3APP chelates can be enriched and separated on a valve switching HPLC system combined with on-line enrichment technique. The linearity ranges are 0.01-120 microg/l for each metal ion. The detection limits (S/N = 3) of lead, cadmium and mercury are 1.2 ng/l, 0.5 ng/l and 0.8 ng/l, respectively. This method was applied to the determination of lead, cadmium and mercury in foodstuffs with good results.
Huang
Zhangjie
Z
Department of Chemistry, Yunnan University, Kunming 650091, P. R. China.
Yang
Guangyu
G
Hu
Qiufen
Q
Yin
Jiayuan
J
D002104:Cadmium / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000592:standards; D004867:Equipment Design; D005506:Food Contamination / Q000032:analysis*; D015386:Hazardous Substances / Q000032:analysis; D007854:Lead / Q000032:analysis; D008628:Mercury / Q000032:analysis; D019216:Metals, Heavy / Q000032:analysis*; D008872:Microwaves; D009862:Online Systems; D012680:Sensitivity and Specificity
D015386:Hazardous Substances; D019216:Metals, Heavy; D002104:Cadmium; D007854:Lead; D008628:Mercury