title: Caging proteins through unnatural amino acid mutagenesis.
abstract: The caging of specific residues of proteins is a powerful tool. This discussion attempts to alert the reader to the considerations that must be made in preparing and analyzing a caged protein through nonsense suppression. Although the suppression methodology is conceptually straightforward, it not possible to provide a failsafe "cook book" method for using caged unnaturals. We have emphasized the preparation of caged receptors expressed in Xenopus oocytes, but these approaches can clearly be adapted to many other systems.
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125, USA.
authors.forename: E James
authors.initials: EJ
authors.lastname: Petersson
authors.forename: Gabriel S
authors.initials: GS
authors.lastname: Brandt
authors.forename: Niki M
authors.initials: NM
authors.lastname: Zacharias
authors.forename: Dennis A
authors.initials: DA
authors.lastname: Dougherty
authors.forename: Henry A
authors.initials: HA
authors.lastname: Lester
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000235:genetics; D000818:Animals; D018388:Codon, Terminator; D016296:Mutagenesis; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D014981:Xenopus
chemical_list: D000596:Amino Acids; D018388:Codon, Terminator; D012333:RNA, Messenger