Microsatellite dinucleotide (T-G) repeat: a candidate DNA marker for breast metastasis.
A dinucleotide (T-G) repeat sequence was isolated by comparing DNA from metastatic lymph node and matched normal breast samples from a ductal mammary carcinoma patient using representational difference analysis (RDA) method. Our present study used this metastasis associated DNA sequence (MADS) as a diagnostic probe to screen five patient samples by slot blot method. A new approach to isolate single cells by microdissection, namely single cell microdissection (SCM) was developed to obtain homogeneous population of tumor cells (approximately 1000) from matched primary tumors and corresponding positive lymph nodes of five patients. We isolated DNA from these homogeneous tumor cells and used for the RDA and DNA slot blot experiments. The screening of patient samples showed loss of this MADS in the transition from primary to metastasis in four out of five cases (80%) suggesting its possible role in breast metastasis.
Mukherjee
Bhaskar
B
Metastasis Laboratory, Department of Radiation Oncology, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.
Zhao
Hui
H
Parashar
Bhupesh
B
Sood
Brij M
BM
Mahadevia
Panna S
PS
Klinger
Harold P
HP
Vikram
Bhadrasain
B
Achary
Mohanrao P
MP
D014408:Biomarkers, Tumor / Q000235:genetics*; D015139:Blotting, Southern; D001943:Breast Neoplasms / Q000235:genetics*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics*; D002469:Cell Separation / Q000379:methods; D004273:DNA, Neoplasm / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D005819:Genetic Markers