title: HIV type 1 diversity in France, 1999-2001: molecular characterization of non-B HIV type 1 subtypes and potential impact on susceptibility to antiretroviral drugs.
abstract: Non-B HIV-1 samples collected in France between 1999 and 2001 were sequenced in the env, reverse transcriptase (RT), and protease genes (1) to characterize further the non-B strains circulating in the country, (2) to assess the importance of recombination, and (3) to describe the polymorphism of RT and protease genes and appreciate a possible impact on susceptibility to antiretroviral (ARV) drugs. The results show that, within a background of CRF02_AG predominance, there is a high genetic diversity of non-B isolates, including intersubtype recombinants. There is an extensive polymorphism of protease and RT genes compared with B consensus sequences; we have so far no data indicating that these non-B isolates may have reduced sensitivity to ARV drugs.
authors.affiliation: Laboratoire de Virologie, Hôpital Pellegrin, CHU de Bordeaux, France. herve.fleury@chu-bordeaux.fr
authors.forename: Herve
authors.initials: H
authors.lastname: Fleury
authors.forename: Patricia
authors.initials: P
authors.lastname: Recordon-Pinson
authors.forename: Anne
authors.initials: A
authors.lastname: Caumont
authors.forename: Muriel
authors.initials: M
authors.lastname: Faure
authors.forename: Pierre
authors.initials: P
authors.lastname: Roques
authors.forename: Jean-Cristophe
authors.initials: JC
authors.lastname: Plantier
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Couturier
authors.forename: Dominique
authors.initials: D
authors.lastname: Dormont
authors.forename: Bernard
authors.initials: B
authors.lastname: Masquelier
authors.forename: François
authors.initials: F
authors.lastname: Simon
mesh_terms: D000328:Adult; D019943:Amino Acid Substitution; D019380:Anti-HIV Agents / Q000494:pharmacology* / Q000627:therapeutic use; D024882:Drug Resistance, Viral; D005260:Female; D005602:France; D015752:Genes, env / Q000235:genetics*; D014644:Genetic Variation; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology*; D016333:HIV Protease / Q000737:chemistry / Q000235:genetics*; D054303:HIV Reverse Transcriptase / Q000737:chemistry / Q000235:genetics*; D015497:HIV-1 / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011995:Recombination, Genetic; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D017422:Sequence Analysis, DNA
chemical_list: D019380:Anti-HIV Agents; D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase; D016333:HIV Protease