title: [Effects of +Gx load on energy metabolism of brain tissue in rats].
abstract: Objective. To observe the changes of energy metabolism of brain tissue in rats under +Gx loads, and to explore its possible role in changes of brain function and work efficiency induced by +Gx stress. Method. Forty-five male Wistar rats were randomly divided into control, +5 Gx, +10 Gx, +15 Gx and +20 Gx group. Each group was exposed to the corresponding G value for 3 min. After that, cortical adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP) and lactic acid (LA) content, lactate dehydrogenase (LDH) activity were measured. Result. Compared with the control group, the cortical (LA) content increased significantly after +5 Gx, +10 Gx, +15 Gx and +20 Gx exposure (P<0.01). Cortical ADP content and ratio of ADP/AMP and AMP/ATP increased significantly after +10 Gx, +15 Gx and +20 Gx exposure (P<0.01), whereas ATP content, energy charge and LDH activity decreased significantly (P<0.05 or 0.01). Cortical AMP content increased significantly after +15 Gx and +20 Gx exposure (P<0.05 and 0.01). Conclusion. It is suggested that +Gx load can result in obvious depression of brain energy metabolism, which could be an important reason for the change of brain function and work efficiency induced by +Gx stress.
authors.affiliation: Institute of Space Medico-Engineering, Beijing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Bao-sheng
authors.initials: BS
authors.lastname: Xie
authors.forename: Guang-xing
authors.initials: GX
authors.lastname: You
authors.forename: Xing-hua
authors.initials: XH
authors.lastname: Liu
authors.forename: Sheng-qiang
authors.initials: SQ
authors.lastname: Lu
authors.forename: Wei-fen
authors.initials: WF
authors.lastname: Huang
mesh_terms: D000054:Acceleration; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism*; D004734:Energy Metabolism / Q000502:physiology*; D018471:Hypergravity; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D019344:Lactic Acid; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase