The embryonic Octamer factor 3/4 displays distinct DNA binding specificity from those of other Octamer factors.
The Oct-3/4 is a transcriptional factor expressed rather specifically in inner cell mass and embryonic ectoderm during early embryogenesis in mammals. During the course of characterizing the regulatory element of embryonic transcriptional coactivator UTF1 which is one of the downstream genes of Oct-3/4, we have found that Oct-3/4, but not other Octamer factors, shows unique DNA binding specificity, being able to bind to variant Octamer sequence (5'-ACTAGCAT-3') present in UTF1 regulatory element. Here, we have determined the DNA binding property of Oct-3/4 in a systematic manner using all possible one point mutants of Octamer sequence as well as completely random sequences. These analyses led to the identification of nine distinct nucleotide sequences including the one (5'-ACTAGCAT-3') present in UTF1 regulatory element which serves rather as specific binding site for the Oct-3/4. Moreover, we show that, at least, one of the newly identified sequences, 5'-ATCAGCAT-3', exhibits a transcriptional stimulating activity equivalent to that of the variant Octamer sequence present in the UTF1 regulatory region in embryonic stem cells in a pluripotent-state specific manner.
Nishimoto
Masazumi
M
Division of Developmental Biology, Saitama Medical School Research Center for Genomic Medicine, 1397-1 Yamane Hidaka, Saitama 350-1241, Japan.
Miyagi
Satoru
S
Katayanagi
Tomoko
T
Tomioka
Mizuho
M
Muramatsu
Masami
M
Okuda
Akihiko
A
D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004475:Ectoderm / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D051863:Host Cell Factor C1; D051379:Mice; D050811:Octamer Transcription Factor-1; D050814:Octamer Transcription Factor-3; D050816:Octamer Transcription Factor-6; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D013234:Stem Cells / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
D004268:DNA-Binding Proteins; C497693:Hcfc1 protein, mouse; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; D050814:Octamer Transcription Factor-3; C494166:Pou2f1 protein, mouse; C494180:Pou3f1 protein, mouse; C494174:Pou5f1 protein, mouse; D014157:Transcription Factors; D050816:Octamer Transcription Factor-6; D004247:DNA