Possibility of progenitor cell mobilization during the hematological recovery following peripheral blood stem cell autograft.
Twenty-four patients with hematological malignancies were studied during recovery following autografting in order to establish the proportion of patients that show CD34+ cell mobilization and the kinetics of mobilized CD34-positive cells. The patients showed a peak in peripheral blood (PB) CD34+ cells after a median of 14 days (range 12-20) following reinfusion. According to the number of circulating CD34+ cells, two groups could be clearly distinguished: 17 patients (group A) with <10 PB CD34+ cells/microl (median 1.2, range 0-5) and 7 patients (group B) with >10 CD34+ cells/microl (median 51, range 13-123). Compared to group A, patients of group B showed a faster hematological reconstitution of both polymorphonuclear leukocytes >500/microl (12 vs. 15 days) and platelets >50,000/microl (12 vs. 17 days). The expression of the beta1 integrin CD49d was similar in the two groups of patients, while a lower expression of the beta2 integrin CD11a and a greater expression of the L-selectin CD62L were observed in the PB CD34+ cells of group B patients. Both in the PB and in the BM, the number of CFU-GEMM, CFU- GM, CFU-E and BFU-E of group B was significantly greater than that of group A. However, when the clonogenic potential of a single CD34+ cell was evaluated, no major differences in the number of colonies produced per CD34+ cell were found between the two groups.
De Propris
Maria Stefania
MS
Dipartimento di Biotecnologia Cellulari ed Ematologia, Università La Sapienza, Roma, Italia. Depropris@bce.med.uniroma1.it
Meloni
Giovanna
G
Cordone
Iole
I
Capria
Saveria
S
Bellucci
Roberto
R
Trisolini
Silvia
S
Simone
Francesca
F
de Fabritiis
Paolo
P
D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology; D040881:CD11a Antigen / Q000032:analysis; D002452:Cell Count; D002478:Cells, Cultured; D002648:Child; D002999:Clone Cells / Q000166:cytology; D003114:Colony-Forming Units Assay; D004912:Erythrocytes; D005260:Female; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D006098:Granulocytes; D019337:Hematologic Neoplasms / Q000628:therapy*; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D039441:Integrin alpha4 / Q000032:analysis; D007700:Kinetics; D019041:L-Selectin / Q000032:analysis; D008264:Macrophages; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D014182:Transplantation, Autologous
D018952:Antigens, CD34; D040881:CD11a Antigen; D019041:L-Selectin; D016179:Granulocyte Colony-Stimulating Factor; D039441:Integrin alpha4