Indirect 17(O)-magnetic resonance imaging of cerebral blood flow in the rat.
Proton T(1rho)-dispersion MRI is demonstrated for indirect, in vivo detection of (17)O in the brain. This technique, which may be readily implemented on any clinical MRI scanner, is applied towards high-resolution, quantitative mapping of cerebral blood flow (CBF) in the rat by monitoring the clearance of (17)O-enriched water. Strategies are derived and employed for 1) quantitation of absolute H(2) (17)O tracer concentration from a ratio of high- and low-frequency spin-locked T(1rho) images, and 2) mapping CBF without having to transform the T(1rho) signal to H(2) (17)O tracer concentration. Absolute regional blood flow was mapped in a single 3-mm brain slice at an in-plane resolution of 0.4 x 0.8 mm within a 5-min tracer washout time; these data are consistent with the less localized CBF measurements reported in the literature. T(1rho)-weighted imaging yields excellent signal-to-noise ratios, spatiotemporal resolution, and anatomical contrast for mapping CBF.
Tailor
Dharmesh R
DR
Metabolic Magnetic Resonance Research and Computing Center, Department of Radiology, University of Pennsylvania, Philadelphia 19104, USA. dtailor@mail.mmrrcc.upenn.edu
Roy
Arijit
A
Regatte
Ravinder R
RR
Charagundla
Sridhar R
SR
McLaughlin
Alan C
AC
Leigh
John S
JS
Reddy
Ravinder
R
D000818:Animals; D002560:Cerebrovascular Circulation; D005240:Feasibility Studies; D005260:Female; D008279:Magnetic Resonance Imaging / Q000379:methods*; D010103:Oxygen Isotopes; D051381:Rats; D017207:Rats, Sprague-Dawley; D014867:Water / Q000378:metabolism
D010103:Oxygen Isotopes; D014867:Water