Genes regulated by learning in the hippocampus.
The enduring changes in long-term memory probably depend on regulation of gene expression in the hippocampus. To seek genes regulated by learning, we used microarray technology to compare hippocampal gene expression in mice undergoing training in the Morris water maze and control mice forced to swim for the same period in the absence of a hidden platform. ANOVA was employed to prioritize genes for further study, and three genes were confirmed by real-time PCR as being regulated during learning. One of the genes was the alpha subunit of the platelet-derived growth factor receptor (Pdgfra); another showed homology to DnaJ and cAMP response element-binding protein 2 (CREB2); and a third was novel. These genes may provide useful insights into the molecular mechanisms of hippocampal learning.
Leil
Tarek A
TA
Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, UCLA School of Medicine, Los Angeles, California, USA.
Ossadtchi
Alex
A
Nichols
Thomas E
TE
Leahy
Richard M
RM
Smith
Desmond J
DJ
D051701:Activating Transcription Factor 4; D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics; D006624:Hippocampus / Q000502:physiology*; D008297:Male; D018782:Maze Learning / Q000502:physiology*; D008568:Memory / Q000502:physiology; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017385:Sequence Homology; D014157:Transcription Factors / Q000235:genetics
D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D014157:Transcription Factors; D051701:Activating Transcription Factor 4; D020796:Receptor, Platelet-Derived Growth Factor alpha