PUVA apoptotic response in activated and resting human lymphocytes.
In extracorporeal photopheresis (ECP) collected cells are treated by 8 methoxypsoralen and UVA (PUVA) which induced apoptosis. The mechanism of action of reinfused cell is unclear. A vaccination model postulates an efficient presentation of apoptotic alloreactive cells to the patient immune system. The efficiency may depend upon a predominance of apoptotic alloreactive cells after PUVA. Such selectivity could result from their activation. We studied apoptosis in resting and PHA-activated lymphocytes. Both were equally susceptible. Changes in early apoptosis were possibly missed. We evaluated the effect of preincubation before PUVA. During preincubation monocyte could affect lymphocytes susceptibility to apoptosis as an increase of number of apoptotic cells was observed after 72 hours in stimulated and resting cells. Our findings do not preclude a selectivity of other PUVA effects since expression of membrane marker also targets to PUVA is modified by activation.
Heng
A E
AE
Service de Néphrologie (Pr Deteix) Henri Dunant BP69 63003, CHU, Clermont-Ferrand Cedex, France. aheng@chu-clermontferrand.fr
Sauvezie
B
B
Genestier
L
L
Demeocq
F
F
Dosgilbert
A
A
Deteix
P
P
D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation / Q000502:physiology; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D011701:PUVA Therapy; D010835:Phytohemagglutinins / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism
D010835:Phytohemagglutinins; D013936:Thymidine