Experimental Pneumocystis carinii pneumonia in simian immunodeficiency virus-infected rhesus macaques.
To establish experimental Pneumocystis carinii infection in simian immunodeficiency virus (SIV)-infected macaques as a model of acquired immunodeficiency syndrome (AIDS)-associated P. carinii pneumonia (PCP), SIV-infected macaques were inoculated intrabronchially with macaque-derived P. carinii, and P. carinii-specific polymerase chain reaction (PCR) and flow cytometric analysis of bronchoalveolar lavage fluid were done biweekly for up to 44 weeks after inoculation. All inoculated animals had a P. carinii-specific PCR product after infection. CD8(+) T cells in lung lavage samples from SIV- and P. carinii-coinfected animals increased to >90% of total CD3(+) cells, a pattern associated with naturally acquired P. carinii infection. Progression of disease also was correlated with increased neutrophil infiltration to the lungs. The animals had a protracted period of asymptomatic colonization with P. carinii before progression to PCP. The development of a model of PCP in SIV-infected rhesus macaques provides the means to study AIDS-associated PCP.
Board
Kathryn F
KF
Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA.
Patil
Sangita
S
Lebedeva
Irina
I
Capuano
Saverio
S
Trichel
Anita M
AM
Murphey-Corb
Michael
M
Rajakumar
Premeela A
PA
Flynn
JoAnne L
JL
Haidaris
Constantine G
CG
Norris
Karen A
KA
D017088:AIDS-Related Opportunistic Infections / Q000150:complications* / Q000175:diagnosis / Q000209:etiology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology / Q000821:virology; D017252:CD3 Complex / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D004271:DNA, Fungal / Q000032:analysis; D004195:Disease Models, Animal; D005260:Female; D008168:Lung / Q000382:microbiology / Q000473:pathology; D008253:Macaca mulatta; D008297:Male; D020556:Neutrophil Infiltration; D011010:Pneumocystis / Q000235:genetics / Q000302:isolation & purification; D011020:Pneumonia, Pneumocystis / Q000150:complications* / Q000175:diagnosis / Q000209:etiology; D016133:Polymerase Chain Reaction; D016097:Simian Acquired Immunodeficiency Syndrome / Q000150:complications*; D015302:Simian Immunodeficiency Virus; D013997:Time Factors
D017252:CD3 Complex; D004271:DNA, Fungal