title: Enzyme inhibitors to increase poly-3-hydroxybutyrate production by transgenic tobacco.
abstract: Chemical regulation of secondary-metabolite synthesis was investigated through the improvement of poly-3-hydroxybutyrate (PHB) production in transgenic tobacco plants by the use of enzyme inhibitors. Two tobacco lines, BC3 and rCAB8, that produce PHB in both the cytosol and plastids were used. An acetyl-CoA carboxylase inhibitor, D-(+)-Quizalofop-ethyl, increased PHB accumulation in both lines 2-fold. The accumulation rate of plastidial PHB in the rCAB8 line was 2.5-fold higher than that of cytosolic PHB in the BC3 line. A specific inhibitor of 3-hydroxy-3-methylglutaryl-CoA reductase, mevastatin, also increased PHB accumulation but only in the BC3 line. These results indicated that chemical regulation of the native metabolic flows by the specific enzyme inhibitors increased secondary-metabolite production in the transgenic tobacco plants we used.
authors.affiliation: RIKEN Institute (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan. yoshi@postman.riken.go.jp
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Minoru
authors.initials: M
authors.lastname: Kurano
authors.forename: Yuko
authors.initials: Y
authors.lastname: Arai
authors.forename: Hideo
authors.initials: H
authors.lastname: Nakashita
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Doi
authors.forename: Ron
authors.initials: R
authors.lastname: Usami
authors.forename: Kohki
authors.initials: K
authors.lastname: Horikoshi
authors.forename: Isamu
authors.initials: I
authors.lastname: Yamaguchi
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000037:antagonists & inhibitors / Q000378:metabolism; D006885:Hydroxybutyrates / Q000378:metabolism*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D008148:Lovastatin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D030821:Plants, Genetically Modified; D011091:Polyesters / Q000378:metabolism*; D011422:Propionates / Q000494:pharmacology*; D011810:Quinoxalines / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
chemical_list: D000103:Acetyl-CoA Carboxylase; D006885:Hydroxybutyrates; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D008148:Lovastatin; D011091:Polyesters; D011422:Propionates; D011810:Quinoxalines; C012258:mevastatin; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C064835:quizalofop-ethyl