An abundant periplasmic protein of the denitrifying phototroph Rhodobacter sphaeroides f. sp. denitrificans is PstS, a component of an ABC phosphate transport system.
To understand a physiological role of an abundant 34-kDa periplasmic protein in the denitrifying phototroph Rhodobacter sphaeroides f. sp. denitrificans grown in a medium containing malate as the carbon source, the gene for the protein was isolated. The deduced amino acid sequence of the protein had a sequence similarity of 66.2% to that of PstS from Sinorhizobium meliloti. The downstream sequence of the Rhodobacter pstS contained five genes similar to pstCAB and phoUB, and its upstream sequence contained a putative regulatory sequence that is analogous to the Pho box involved in phosphate-limitation-induced gene expression in Escherichia coli. Both the amount of the PstS and the pstS promoter-driven expression of lacZ activity increased about two-fold in response to phosphate limitation. This is the first isolation of pst genes encoding proteins of an ABC phosphate transporter system from phototrophic bacteria.
Matsuzaki
Masahiro
M
Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima, 739-8526 Japan. mmatsuza@hiroshima-u.ac.jp
Abe
Miho
M
Hara
Satomi
S
Iwasaki
Yoshiko
Y
Yamamoto
Isamu
I
Satoh
Toshio
T
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D008293:Malates / Q000494:pharmacology; D008969:Molecular Sequence Data; D033903:Periplasmic Proteins / Q000235:genetics / Q000378:metabolism*; D028061:Phosphate Transport Proteins / Q000378:metabolism*; D010710:Phosphates / Q000494:pharmacology; D012242:Rhodobacter sphaeroides / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D008293:Malates; D033903:Periplasmic Proteins; D028061:Phosphate Transport Proteins; D010710:Phosphates; C030298:malic acid