[The effect of monocytes on early activation of lymphocytes T among children with idiopathic uveitis].
OBJECTIVE
We have investigated T Lymphocytes ability for CD69 molecule induction in presence and absence of monocytes in idiopathic uveitis.


METHODS
Twenty-five children with idiopathic uveitis were studied. The control group consisted of 12 healthy children. Peripheral blood mononuclear cells (PBMCs) were isolated from heparinised venous blood, by density gradient centrifugation. CD69 expression was cytometrically assessed on freshly isolated and cultured T lymphocytes.


RESULTS
CD69 expression on freshly isolated peripheral blood T lymphocytes was low in both studied groups. LPS-stimulated monocytes added to cultures of T lymphocytes induced increase in CD69 expression but significantly lower in children with idiopathic uveitis compare to healthy children.
Bakunowicz-Łazarczyk
Alina
A
Kliniki Okulistyki Dzieciecej Samodzielnego Publicznego Dzieciecego Szpitala Klinicznego Akademii Medycznej w Białymstoku.
Moniuszko
Tadeusz
T
Sredzińska-Kita
Dorota
D
Mrugacz
Małgorzata
M
D000293:Adolescent; D015703:Antigens, CD / Q000097:blood; D000945:Antigens, Differentiation, T-Lymphocyte / Q000097:blood; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002648:Child; D005260:Female; D006801:Humans; D037181:Lectins, C-Type; D008213:Lymphocyte Activation; D008297:Male; D009000:Monocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors; D014605:Uveitis / Q000276:immunology*; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D015415:Biomarkers; D037181:Lectins, C-Type; D000099192:CD69 Antigens