Antigenic hepatitis A virus structures may be produced in Escherichia coli.
The synthesis of 14S pentamers and 70S empty capsids of hepatitis A virus (HAV) has been accomplished by expressing the viral genome for periods of time longer than 4 h in Escherichia coli. HAV pentamers (14S) self-assembled into capsids (70S) in vitro. The antibodies induced by these structures recognized and neutralized HAV.
Sánchez
Glòria
G
Enteric Virus Laboratory, Department of Microbiology, University of Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain.
Caballero
Santiago
S
Guix
Susana
S
Bosch
Albert
A
Pintó
Rosa M
RM
D000818:Animals; D002213:Capsid / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005260:Female; D035922:Hepatitis A Antibodies / Q000097:blood; D035921:Hepatitis A Antigens / Q000276:immunology / Q000378:metabolism*; D030041:Hepatitis A virus / Q000276:immunology / Q000378:metabolism; D051379:Mice; D009500:Neutralization Tests; D010957:Plasmids
D035922:Hepatitis A Antibodies; D035921:Hepatitis A Antigens