[A detection method for recombinant DNA from genetically modified potato (NewLeaf Y potato)].
A detection method using the polymerase chain reaction (PCR) was developed to detect genetically modified (GM) potato (NewLeaf Y potato; NL-Y), of which the mandatory assessment has not yet been completed in Japan. The potato sucrose synthase gene was used as an internal control. We designed a primer pair to specifically detect NL-Y without false-positive results in processed potato foods infected with the potato virus Y (PVY). The DNA introduced into NL-Y using the primer pair could be detected from potato powder samples containing 0.05% NL-Y. In addition, we designed primer pairs for recognizing the CryIIIA gene to detect the NewLeaf potato (NL), NewLeaf Plus potato (NL-P) and NL-Y and for recognizing p-FMV in order to detect NL-P and NL-Y. The proposed method was applied to the detection of NL-Y in 26 processed potato foods and NL-Y was not detected in any samples.
Akiyama
Hiroshi
H
National Institute of Health Sciences: 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Watanabe
Takahiro
T
Wakui
Chiseko
C
Chiba
Yoshiko
Y
Shibuya
Masaaki
M
Goda
Yukihiro
Y
Toyoda
Masatake
M
D017931:DNA Primers / Q000235:genetics; D004274:DNA, Recombinant / Q000032:analysis*; D030841:Food, Genetically Modified; D016133:Polymerase Chain Reaction; D017800:Potyvirus / Q000235:genetics; D011198:Solanum tuberosum / Q000235:genetics*
D017931:DNA Primers; D004274:DNA, Recombinant