title: Molecular profiles of invasive mucinous and ductal carcinomas of the breast: a molecular case study.
abstract: Expression profiling using cDNA microarrays have redefined the molecular classification of some cancers. The comprehensive genetic analysis also permits the identification of novel pathways that might determine subtle differences in tumor phenotype. Herein, we analyzed the tissues from a patient with bilateral cancer of different histologies in each breast (pure invasive mucinous and pure invasive ductal), thus providing a unique opportunity to assess the expression profiles determined by histology in an isogenic human background. Our results show that the mucinous phenotype is associated with the expression of immunostimulatory and inhibitory genes, consistent with the cellular infiltration of lymphocytes and with the expression of enzymes involved in mucin production. Moreover, the panel of matrix metallo-proteinases are distinctly different between the mucinous and the invasive tumors, suggesting that therapeutic targets to this class of compounds may need to be tailored for the varying histologies. Taken together, these data suggest that expression profiling can be used diagnostically to distinguish individual histologic subclassifications and may guide the selection of target therapeutics.
authors.affiliation: Department of Breast Medical Oncology, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA. lpusztai@mdanderson.org
authors.forename: Lajos
authors.initials: L
authors.lastname: Pusztai
authors.forename: Christos
authors.initials: C
authors.lastname: Sotiriou
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Buchholz
authors.forename: Funda
authors.initials: F
authors.lastname: Meric
authors.forename: W Fraser
authors.initials: WF
authors.lastname: Symmans
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Esteva
authors.forename: Aysegul
authors.initials: A
authors.lastname: Sahin
authors.forename: Edison T
authors.initials: ET
authors.lastname: Liu
authors.forename: Gabriel N
authors.initials: GN
authors.lastname: Hortobagi
mesh_terms: D002288:Adenocarcinoma, Mucinous / Q000235:genetics*; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis