title: [Expression of a new lung cancer drug resistance-related gene in lung cancer tissues and lung cancer cell strains].
abstract: OBJECTIVE
  A new drug resistance-related gene fragment which was 494 bp long was found using suppression subtractive hybridization (SSH) and its full-length cDNA fragment was cloned by the authors. This study was designed to determine the expression of this lung cancer drug resistance-related gene (LCDRG) in lung cancer tissues, juxtacancerous tissues, and five lung cancer cell strains.
  
  
  METHODS
  The expression of LCDRG was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) method in 38 lung cancer tissues,12 juxtacancerous tissues, and 5 lung cancer cell strains.
  
  
  RESULTS
  The expression of LCDRG in cancer tissues was significantly higher than that in juxtacancerous tissue (P< 0.001). There was no significance of the expression among adenocarcinoma, brochioloalveolar carcinoma, and squamous carcinoma. In lung cancer cell strains, the expression levels of LCDRG in adenocarcinoma cell strains SPC-A-1 and A549, big cell lung cancer cell strain H460, small cell lung cancer cell strains H446 and SH77 were decreased gradually.
  
  
  CONCLUSIONS
  LCDRG is closely related to lung cancer and may be involved in the pathogenesis of lung cancer.
authors.affiliation: Institute of Respiratory Disease, Xinqiao Hospital, The Third Military Medical University, Chongqing, 400037, PR China.
authors.forename: Ling-Zhi
authors.initials: LZ
authors.lastname: Liu
authors.forename: Gui-Sheng
authors.initials: GS
authors.lastname: Qian
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D009363:Neoplasm Proteins