title: Compartmentalized production of CCL17 in vivo: strong inducibility in peripheral dendritic cells contrasts selective absence from the spleen.
abstract: Dendritic cells (DCs)(*) fulfill an important regulatory function at the interface of the innate and adaptive immune system. The thymus and activation-regulated chemokine (TARC/CCL17) is produced by DCs and facilitates the attraction of activated T cells. Using a fluorescence-based in vivo reporter system, we show that CCL17 expression in mice is found in activated Langerhans cells and mature DCs located in various lymphoid and nonlymphoid organs, and is up-regulated after stimulation with Toll-like receptor ligands. DCs expressing CCL17 belong to the CD11b(+)CD8(-)Dec205(+) DC subset, including the myeloid-related DCs located in the subepithelial dome of Peyer's patches. CCL17-deficient mice mount diminished T cell-dependent contact hypersensitivity responses and display a deficiency in rejection of allogeneic organ transplants. In contrast to lymphoid organs located at external barriers of the skin and mucosa, CCL17 is not expressed in the spleen, even after systemic microbial challenge or after in vitro stimulation. These findings indicate that CCL17 production is a hallmark of local DC stimulation in peripheral organs but is absent from the spleen as a filter of blood-borne antigens.
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, D-81675 Munich, Germany.
authors.forename: Judith
authors.initials: J
authors.lastname: Alferink
authors.forename: Ivo
authors.initials: I
authors.lastname: Lieberam
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Reindl
authors.forename: Andrea
authors.initials: A
authors.lastname: Behrens
authors.forename: Susanne
authors.initials: S
authors.lastname: Weiss
authors.forename: Norbert
authors.initials: N
authors.lastname: Hüser
authors.forename: Klaus
authors.initials: K
authors.lastname: Gerauer
authors.forename: Ralf
authors.initials: R
authors.lastname: Ross
authors.forename: Angelika B
authors.initials: AB
authors.lastname: Reske-Kunz
authors.forename: Parviz
authors.initials: P
authors.lastname: Ahmad-Nejad
authors.forename: Hermann
authors.initials: H
authors.lastname: Wagner
authors.forename: Irmgard
authors.initials: I
authors.lastname: Förster
mesh_terms: D000818:Animals; D039521:CD11c Antigen / Q000378:metabolism; D054414:Chemokine CCL17; D019742:Chemokines, CC / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D003877:Dermatitis, Contact / Q000276:immunology; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology / Q000378:metabolism; D018390:Gene Targeting; D017930:Genes, Reporter; D006085:Graft Survival; D049452:Green Fluorescent Proteins; D016027:Heart Transplantation; D007801:Langerhans Cells / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D008088:Listeriosis / Q000276:immunology; D008164:Luminescent Proteins / Q000378:metabolism; D008221:Lymphoid Tissue / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010587:Phagocytosis; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism*
chemical_list: D039521:CD11c Antigen; C499538:Ccl17 protein, mouse; D054414:Chemokine CCL17; D019742:Chemokines, CC; D008070:Lipopolysaccharides; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins