Optimization of cationic lipid mediated gene transfer: structure-function, physico-chemical, and cellular studies.
The rationale design aimed at the enhancement of cationic lipid mediated gene transfer is discussed. These improvements are based on the straight evaluation of the structure-activity relationship and on the introduction of new structures. Much attention have been given to the supramolecular structures of the lipid/DNA complexes, to the effect of serum on gene transfer and to the intracellular trafficking of the lipoplexes. Finally new avenue using reducible cationic lipids has been discussed.
Carrière
Marie
M
Laboratoire de Chimie Bioorganique et de Biotechnologie Moléculaire et Cellulaire, UMR 7001 CNRS ENSCP, Aventis ENSCP, Paris, France.
Tranchant
Isabelle
I
Niore
Pierre-Antoine
PA
Byk
Gerardo
G
Mignet
Nathalie
N
Escriou
Virginie
V
Scherman
Daniel
D
Herscovici
Jean
J
D016475:3T3 Cells; D000818:Animals; D002412:Cations; D004247:DNA / Q000378:metabolism; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D050356:Lipid Metabolism; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D008956:Models, Chemical; D011073:Polyamines / Q000737:chemistry; D013329:Structure-Activity Relationship; D014162:Transfection
D002412:Cations; D011073:Polyamines; D004247:DNA; D008156:Luciferases