Native state, energetic interaction of chlorophyll precursors and intraplastid location of S-adenosyl-L-methionine: Mg-protoporphyrin IX methyltransferase in etiolated leaves.
Low temperature fluorescence spectra (FS) and fluorescence excitation spectra (FES) of protoporphyrin IX (Proto), Mg-protoporphyrin IX and its monomethyl ester (MgProto-ME) and protochlorophyllide (Pchlide) in etiolated barley leaves treated with 5-aminolevulinic acid and/or 2,2'-dipyridyl were studied. The spectra of Proto and MgProto-ME showed a little dependence on temperature of registration and exhibited similarity to low temperature spectra in diluted organic and buffer solutions. However, a red wavelength shift for Soret bands of Proto and MgProto-ME was observed due to porphyrin interaction with bovine serum albumin in 0.05 M, Na2HPO4 solution at room temperature. Disaggregating treatments had no effect on Proto and MgProto-ME spectra in plants. These results suggested that in etiolated leaves Proto and MgProto-ME molecules were in a monomer state. The spectral properties of these molecules were determined by interaction of porphyrins with proteins and other plastid membrane components. The spectral analyses indicated an efficient energy migration from Proto and MgProto-ME molecules to active form of Pchlide which emitted at 656nm, and no energy transfer from carotenoids to porphyrins in vivo. These findings suggested that Proto and MgProto-ME from carotenoids, and close location of these porphyrins and photoactive Pchlide in etioplast membranes. The latter conclusion was strongly supported by an observation that in etiolated leaves, S-adenosyl-L-methionin:Mg-protoporphyrin IX methyltransferase, which converts MgProto into MgProtoME, were located not only in prothylakoids but also in prolamellar bodies containing photoactive Pchlide.
Averina
Natalia
N
Institute of Photobiology, National Academy of Sciences of Belarus, Akademicheskaya St. 27, Minsk 220072, Belarus. ipb@biobel.bas-net.by
Rassadina
Valentina
V
Leonid
Fradkin
F
D002734:Chlorophyll / Q000737:chemistry* / Q000378:metabolism; D001467:Hordeum / Q000378:metabolism; D008780:Methyltransferases / Q000737:chemistry* / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D011166:Porphyrins / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence / Q000379:methods; D013053:Spectrophotometry; D013696:Temperature; D013997:Time Factors
D011166:Porphyrins; D002734:Chlorophyll; D008780:Methyltransferases; C060234:magnesium-protoporphyrin methyltransferase