Molecular characterization of Llchit1, a midgut chitinase cDNA from the leishmaniasis vector Lutzomyia longipalpis.
During development within the midgut of the sand fly vector, Leishmania parasites after undergoing differentiation and multiplication must escape the peritrophic matrix (PM). Although Leishmania chitinase is believed to take part in promoting the escape of the parasite from the PM by inducing degradation of chitin fibers, it is conceivable that a sand fly-derived chitinase can also have a role in such an event. Here we describe the molecular cloning and partial characterization of a complete cDNA from a putative gut-specific, blood-induced chitinase from the sand fly vector Lutzomyia longipalpis. Llchit1 has an ORF of 1425 bp that encodes a predicted 51.6 kDa mature protein showing high similarity with chitinases from several different organisms. Messenger RNA expression studies indicate that Llchit1 is detected only in the blood fed midgut and it seems to reach a peak at approximately 72 h post blood meal (PBM). To date, only one midgut-specific chitinase from an insect disease vector, AgChi-1 from Anopheles gambiae, has been characterized. As with its mosquito counterpart, Llchit1 can be a target for development of a transmission blocking vaccine.
Ramalho-Ortigão
J M
JM
Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil 4365, 22461-900, Rio de Janeiro RJ, Brazil. mortigao@niaid.nih.gov
Traub-Csekö
Y M
YM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002688:Chitinases / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004175:Diptera / Q000469:parasitology*; D007303:Insect Vectors / Q000469:parasitology*; D007896:Leishmaniasis / Q000635:transmission*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D002688:Chitinases