title: Use of pi-acceptors for spectrophotometric determination of dicyclomine hydrochloride.
abstract: Simple, rapid, accurate, and sensitive spectrophotometric methods are described for the determination of dicyclomine hydrochloride. The methods are based on the reaction of this drug as an n-electron donor with 2,3-dichloro-5,6-dicyano-p-benzoqunione (DDQ), p-chloranilic acid (p-CA), and chloranil (CL) as pi-acceptors to give highly colored complex species. The colored products are measured spectrophotometrically at 456, 530, and 650 nm for DDQ, p-CA, and CL, respectively. Optimization of the different experimental conditions were studied. Beer's law was obeyed in concentration ranges of 20-100, 50-250, and 80-600 microg/mL for DDQ, pCA, and CL, respectively. Colored complexes are produced in organic solvents and are stable for at least 1 h. The methods were applied to Spasmorest antispasmotic tablets and ampoules with good accuracy and precision.
authors.affiliation: National Organization for Drug Control and Research, 6 Hussen Kamal El-Deen, Ben el Sariat, Dokki, Giza 12311, Egypt. bebawy_lories@yahoo.com
authors.forename: Lories I
authors.initials: LI
authors.lastname: Bebawy
authors.forename: Yosry M
authors.initials: YM
authors.lastname: Issa
authors.forename: Kamal M
authors.initials: KM
authors.lastname: Abdel Moneim
mesh_terms: D016227:Benzoquinones / Q000737:chemistry; D002703:Chloranil / Q000737:chemistry; D003124:Colorimetry; D004025:Dicyclomine / Q000032:analysis* / Q000737:chemistry; D007202:Indicators and Reagents; D007700:Kinetics; D018727:Muscarinic Antagonists / Q000032:analysis* / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000737:chemistry; D012680:Sensitivity and Specificity; D012997:Solvents; D013053:Spectrophotometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D016227:Benzoquinones; D007202:Indicators and Reagents; D018727:Muscarinic Antagonists; D004364:Pharmaceutical Preparations; D012997:Solvents; D002703:Chloranil; C000928:dichlorodicyanobenzoquinone; D004025:Dicyclomine; C014400:chloranilic acid