title: Sporotomaculum syntrophicum sp. nov., a novel anaerobic, syntrophic benzoate-degrading bacterium isolated from methanogenic sludge treating wastewater from terephthalate manufacturing.
abstract: An anaerobic, mesophilic, syntrophic benzoate-degrading bacterium, designated strain FB(T), was isolated from methanogenic sludge which had been used to treat wastewater from the manufacture of terephthalic acid. Cells were non-motile gram-positive rods that formed spores. The optimum temperature for growth was 35-40 degrees C, and the optimum pH was 7.0-7.2. A co-culture with the hydrogenotrophic methanogen Methanospirillum hungatei converted benzoate to acetate, carbon dioxide, and methane. Butyrate transiently accumulated at a high concentration of 2.5 mM during degradation. Besides benzoate, no other compound tested supported growth of the co-culture. Crotonate supported growth of strain FB(T) in pure culture. Furthermore, the strain degraded benzoate in pure culture with crotonate as co-substrate to produce acetate and butyrate. The strain was not able to utilize sulfate, sulfite, thiosulfate, nitrate, fumarate, or Fe(III) as electron acceptor. The G+C content of the DNA was 46.8 mol%. Strain FB(T) contained MK-7 as the major quinone and C(16:1) as the major fatty acid. 16S rDNA sequence analysis revealed that the strain was a member of the genus Sporotomaculum, even though it exhibited significant differences, such as the capacity for syntrophic growth, to the known member of the genus. Hence, we propose the name Sporotomaculum syntrophicum sp. nov. for strain FB(T). The type strain is strain FB(T) (DSM 14795, JCM 11475).
authors.affiliation: Department of Environmental Systems Engineering, Nagaoka University of Technology, 940-2188, Nagaoka, Niigata, Japan.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Qiu
authors.forename: Yuji
authors.initials: Y
authors.lastname: Sekiguchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Imachi
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kamagata
authors.forename: I-Cheng
authors.initials: IC
authors.lastname: Tseng
authors.forename: Sheng-Shung
authors.initials: SS
authors.lastname: Cheng
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Ohashi
authors.forename: Hideki
authors.initials: H
authors.lastname: Harada
mesh_terms: D001421:Bacteria, Anaerobic / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001565:Benzoates / Q000032:analysis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003437:Crotonates / Q000032:analysis / Q000378:metabolism; D020564:Deltaproteobacteria / Q000378:metabolism; D004783:Environmental Microbiology; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000032:analysis; D019639:Methanospirillum / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D010795:Phthalic Acids / Q000138:chemical synthesis*; D010802:Phylogeny; D012722:Sewage / Q000382:microbiology; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D001565:Benzoates; D003437:Crotonates; D007220:Industrial Waste; D010795:Phthalic Acids; D012722:Sewage; C011363:terephthalic acid