Phosphatidylserine externalization during bone marrow cell death after treatment of mice with WR-2721 and gamma-rays.
The cell surface exposure of phosphatidylserine (PS) and the plasma membrane impairment were assessed in the bone marrow of adult male Swiss mice exposed to a single 6 Gy dose of 60 Co gamma-rays, and treated intraperitoneally with the aminothiol WR-2721 (Amifostine, S-2-/3-aminopropylamino/ethyl phosphorothioic acid), at a dose of 400 mg/kg body weight, 30 min prior to gamma-irradiation. The bone marrow cells were stained with a combination of fluoresceinated annexin V (annexin V--FITC) and propidium iodide (PI) at 3 h, 7 h, and 24 h after treatment of mice with WR-2721 and 60Co gamma-irradiation. The number of early apoptotic cells (annexin V--FITC positive/PI negative), and late apoptotic and necrotic cells (annexin V--FITC positive/PI positive), was increased at 3 h after exposure of mice to 60Co gamma-rays and thereafter declined with the frequency of apoptotic and necrotic cells remaining lower in WR-2721 pre-treated mice. Using the annexin V--FITC flow cytometric assay, the radioprotective effect of WR-2721 against induction of apoptosis and necrosis in normal cells of the haematopoietic system was shown.
Mazur
Lidia
L
Department of Animal Physiology, Laboratory of Experimental Haematology and Toxicology, Institute of Zoology, Jagiellonian University, R. Ingardena 6, 30-060 Kraków, Poland. maz@zuk.iz.uj.edu.pl
Bochenek
Michał
M
Augustynek
Anna
A
D004999:Amifostine / Q000494:pharmacology*; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D008297:Male; D051379:Mice; D010718:Phosphatidylserines / Q000378:metabolism*
D010718:Phosphatidylserines; D004999:Amifostine