Assessing gene expression variation in normal human tissues using GeneTag, a novel, global, sensitive profiling method.
GeneTag is a novel expression profiling method that allows the visualization, quantification and identification of expressed genes-whether known or novel-in any species, tissue or cell type, independent of knowledge of the underlying sequence. Here we describe the application of this method to determine variation of gene expression in individual human liver samples and the identification of tissue-specific genes by comparing expression patterns across several human organs. Expression data are stored in a database for future reference and data analysis relies on proprietary software, which allows complex comparisons to be performed. Differentially expressed genes are quickly identified through a link to a sequence database. The results from our study underscore the importance of knowledge of individual variation of gene expression for the design and interpretation of transcript profiling experiments in the context of any biological question.
Wong
Lily Y
LY
Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA.
Hafeman
Andrea
A
Boyd
Victoria L
VL
Bodeau
John
J
Lazaruk
Katherine D
KD
Liew
Sueh-Ning
SN
Casey
Peter
P
Belonogoff
Victor
V
Bit
Somaya
S
Sumner
Craig
C
Bredo
Alisa
A
Ho
Norman
N
Chu
Erwin
E
Olson
Sheri
S
Rabkin
Steven
S
Maltchenko
Sergei
S
Spier
Gene
G
Gilbert
Dennis
D
Baumhueter
Susanne
S
D001921:Brain; D001923:Brain Chemistry; D016172:DNA Fingerprinting / Q000379:methods; D018076:DNA, Complementary / Q000032:analysis; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008099:Liver / Q000737:chemistry* / Q000196:embryology; D008168:Lung / Q000737:chemistry; D008297:Male; D009928:Organ Specificity; D010920:Placenta / Q000737:chemistry; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016415:Sequence Alignment / Q000379:methods*; D014158:Transcription, Genetic / Q000235:genetics
D018076:DNA, Complementary; D012333:RNA, Messenger