[Study on expression of beta-NGF in myoblasts transfected by PSVCEP NGF-CAT with Lipofect AMINE].
OBJECTIVE
To gain an understanding of the expression of beta-NGF in myoblasts transfected by PSVCEP NGF-CAT and the transfecting efficiency of Lipofect AMINE.


METHODS
PSVCEP NGF-CAT was delivered into cultured myoblasts by Lipofect AMINE. Expression of beta-NGF in the transfected myoblasts was detected by immunocytochemistry.


RESULTS
Lipofect AMINE was evidently engulfed by myoblasts at the 6th hour; about 40% myoblasts could be detected with the expression of beta-NGF by immunocytochemistry at the 48th hour.


CONCLUSIONS
PSVCEP NGF-CAT can express beta-NGF in cultured myoblasts, and Lipofect AMINE is a convenient vector for gene transfection with simplicity and efficacy.
Mao
Boyong
B
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Liu
Yanhui
Y
Chen
Junjie
J
Wang
Ruohan
R
Cheng
Yongzhong
Y
Gao
Lida
L
He
Min
M
Li
Shengfu
S
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D010714:Phosphatidylethanolamines; D051381:Rats; D017208:Rats, Wistar; D014162:Transfection
D010714:Phosphatidylethanolamines; C020888:1,2-dielaidoylphosphatidylethanolamine; D020932:Nerve Growth Factor