title: Relationship of the fistulas to the rectum and genitourinary tract in mouse fetuses with high anorectal malformations induced by all-trans retinoic acid.
abstract: Since high anorectal malformations with fistulae in human embryos and fetuses of successive developmental stages have not been reported, the embryologic relationship between the rectal fistula (RF) and the genitourinary tract (GUT) in high anorectal agenesis (ARA) remains to be elucidated. This study investigates the developmental relationship between the RF and the GUT in male and female fetuses with high ARA using our established model for high ARA with fistula in mice. Pregnant mice received all-trans retinoic acid suspended in corn oil (5 mg/ml) 100 mg/kg i.p. on day 9 of pregnancy. All fetuses were removed from the uterus on a single day from days 12 to 18 of pregnancy. The caudal regions were analyzed histologically with hematoxylin and eosin staining. All fetuses examined had high ARA with fistula. On day 12 of pregnancy, an anomalous communication was seen between the urogenital sinus (UGS) and the rectum. In the affected female fetuses, on day 14 of pregnancy the paramesonephric (müllerian) ducts and müllerian tubercle were located above the rectocloacal fistula (RCF), and on day 18 of pregnancy the uterovaginal canal was located between the cloaca and the RCF. In the male fetuses, on day 14 of pregnancy the junction between the mesonephric (wolffian) duct and the UGS was located away from the junction between the rectum and the UGS. On day 18 of pregnancy the ejaculatory duct was located between the urinary bladder and the rectourethral fistula. The results of our experiment clearly show the embryologic relationship between the RF and the GUT with high ARA. The anomalous communication between the UGS and the rectum may interfere with normal caudal migration along the dorsal wall of the UGS at the junction between the UGS and the mesonephric or paramesonephric duct.
authors.affiliation: Department of Teratology and Genetics, Research Institute of environmental Medicine, Nagoya University, Nagoya 464-8601, Japan.
authors.forename: Ryozo
authors.initials: R
authors.lastname: Hashimoto
authors.forename: M
authors.initials: M
authors.lastname: Nagaya
authors.forename: Y
authors.initials: Y
authors.lastname: Ishiguro
authors.forename: M
authors.initials: M
authors.lastname: Inouye
authors.forename: H
authors.initials: H
authors.lastname: Aoyama
authors.forename: S
authors.initials: S
authors.lastname: Futaki
authors.forename: Y
authors.initials: Y
authors.lastname: Murata
mesh_terms: D001003:Anal Canal / Q000002:abnormalities* / Q000196:embryology; D000818:Animals; D004065:Digestive System Abnormalities / Q000139:chemically induced*; D004195:Disease Models, Animal; D005260:Female; D005333:Fetus / Q000187:drug effects; D008297:Male; D051379:Mice; D011247:Pregnancy; D012003:Rectal Fistula / Q000139:chemically induced*; D012007:Rectum / Q000002:abnormalities* / Q000196:embryology; D014212:Tretinoin / Q000633:toxicity; D014564:Urogenital Abnormalities / Q000139:chemically induced*
chemical_list: D014212:Tretinoin