Inhibition of nitrobenzene-induced DNA and hemoglobin adductions by dietary constituents.
Nitrobenzene (NB), a widely used industrial chemical, is a likely human carcinogen. Many dietary constituents can suppress the DNA-adduction, acting as the inhibitors of cancer. In this study, we investigated the inhibitory effects of vitamin C (VC), vitamin E (VE), tea polyphenols (TP), garlic squeeze, curcumin, and grapestone extract on NB-DNA and NB-hemoglobin (Hb) adductions in mice using an ultrasensitive method of accelerator mass spectrometry (AMS) with 14C-labelled nitrobenzene. All of these dietary constituents showed their inhibitory effects on DNA or Hb adduction. VC, VE, TP and grapestone extract could efficaciously inhibit the adductions by 33-50%, and all of these six agents could inhibit Hb adduction by 30-64%. We also investigated resveratrol, curcumin, VC and VE as inhibitors of NB-DNA adduction in vitro using liquid scintillation counting technique. These agents in the presence of NADPH and S9 components also pronouncedly blocked DNA adduction in a dose-dependent profile. Our study suggests that these seven constituents may interrupt the process of NB-induced chemical carcinogenesis.
Li
Hongli
H
Department of Applied Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, People's Republic of China.
Cheng
Yan
Y
Wang
Haifang
H
Sun
Hongfang
H
Liu
Yuanfang
Y
Liu
Kexin
K
Peng
Shixiang
S
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology; D002273:Carcinogens / Q000633:toxicity*; D004247:DNA / Q000187:drug effects*; D018736:DNA Adducts; D019587:Dietary Supplements; D006454:Hemoglobins / Q000187:drug effects*; D008297:Male; D051379:Mice; D009578:Nitrobenzenes / Q000633:toxicity*; D012588:Scintillation Counting
D000975:Antioxidants; D002273:Carcinogens; D018736:DNA Adducts; D006454:Hemoglobins; D009578:Nitrobenzenes; D004247:DNA; C036077:nitrobenzene