title: Micropropagation of Terminalia arjuna Roxb. from cotyledonary nodes.
abstract: Cotyledonary node explants excised from 21 day old seedlings of T. arjuna produced multiple shoots when cultured on full strength MS or modified MS (1/2 strength major salts and Fe-EDTA) medium supplemented with different concentrations (0.1-1.0 mg/l) of BAP. Maximum 8.9 shoots/explant could be recorded after 30 days of inoculation on modified MS medium supplemented with BAP (0.5 mg/l). A proliferating shoot culture was established by reculturing the original cotyledonary nodes (2-3 times) on shoot multiplication medium after each harvest of the newly formed shoots. Shoots (each having 2-3 nodes/shoot) thus obtained were also used as a source of nodal explant that gave rise to 1-2 shoots when cultured on modified MS+BAP (0.5 mg/l) medium. Thus, 45-55 shoots could be obtained after 60 days of culture initiation from a single cotyledonary node. About 88% shoots rooted well after 15 hr pulse treatment with IBA (1 mg/l) in liquid MS medium followed by transfer to modified MS medium without IBA. About 80% of these plantlets were successfully acclimatized in plastic pots containing sand and soil mixture and 70% plantlets transferred in the field those survived even after 6 months of transplantation.
authors.affiliation: Department of Botany, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Seema
authors.initials: S
authors.lastname: Pandey
authors.forename: V S
authors.initials: VS
authors.lastname: Jaiswal
mesh_terms: D001564:Benzo(a)pyrene / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D007210:Indoleacetic Acids / Q000494:pharmacology; D018520:Plant Shoots / Q000187:drug effects / Q000196:embryology / Q000254:growth & development*; D012038:Regeneration; D036226:Seedlings / Q000737:chemistry; D029766:Terminalia / Q000254:growth & development*
chemical_list: D003470:Culture Media; D007210:Indoleacetic Acids; D001564:Benzo(a)pyrene