title: Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
abstract: Additional targets of CodY, a GTP-activated repressor of early stationary-phase genes in Bacillus subtilis, were identified by combining chromatin immunoprecipitation, DNA microarray hybridization, and gel mobility shift assays. The direct targets of CodY newly identified by this approach included regulatory genes for sporulation, genes that are likely to encode transporters for amino acids and sugars, and the genes for biosynthesis of branched-chain amino acids.
authors.affiliation: Department of Cellular and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
authors.forename: Virginie
authors.initials: V
authors.lastname: Molle
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Nakaura
authors.forename: Robert P
authors.initials: RP
authors.lastname: Shivers
authors.forename: Hirotake
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Richard
authors.initials: R
authors.lastname: Losick
authors.forename: Yasutaro
authors.initials: Y
authors.lastname: Fujita
authors.forename: Abraham L
authors.initials: AL
authors.lastname: Sonenshein
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins; D015964:Gene Expression Regulation, Bacterial; D007763:Lac Operon / Q000235:genetics; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011233:Precipitin Tests / Q000379:methods*; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D002843:Chromatin; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins