title: Toad atrial natriuretic peptide: cDNA cloning and functional analysis in isolated perfused kidneys.
abstract: A complementary DNA (cDNA) encoding Bufo marinus (toad) preproatrial natriuretic peptide (preproANP) was isolated by reverse-transcription polymerase chain reaction. Sequence analysis of toad preproANP cDNA revealed an open reading frame of 150 amino acid residues, which shared 72% and 66% identity with Rana catesbeiana and Xenopus laevis preproANP, respectively. The deduced amino acid sequence of toad ANP that corresponded to ANP 1-24 of R. catesbeiana and Rana ridibunda was identical, but it differed by four residues from that of X. laevis. ANP mRNA transcripts were also shown to be expressed in the toad kidney. Subsequently, the effect of frog ANP (1-24) on renal function in toad was examined using a perfused kidney preparation. The arterial infusion of frog ANP caused a dose-dependent decrease in the arterial perfusion pressure that was associated with an increase in the glomerular filtration rate (GFR) and a renal natriuresis and diuresis. The renal natriuresis and diuresis resulted predominantly from an increased GFR rather than from direct tubular effects. This study demonstrates that ANP can regulate renal function, which suggests it may be involved in overall fluid volume regulation.
authors.affiliation: School of Biological and Chemical Sciences, Deakin University, Geelong, Victoria 3217, Australia. jdonald@deakin.edu.au
authors.forename: John A
authors.initials: JA
authors.lastname: Donald
authors.forename: Stuart K
authors.initials: SK
authors.lastname: Meier
authors.forename: Shane
authors.initials: S
authors.lastname: Riddell
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics* / Q000494:pharmacology*; D001483:Base Sequence; D001794:Blood Pressure / Q000187:drug effects; D002024:Bufo marinus / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D005919:Glomerular Filtration Rate / Q000187:drug effects; D007668:Kidney / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D009924:Organ Culture Techniques; D010477:Perfusion; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D009320:Atrial Natriuretic Factor