Effects of angiotensin II and natriuretic peptides of the eel on prolactin and growth hormone release in the tilapia, Oreochromis mossambicus.
The effects of angiotensin II (ANG II) and natriuretic peptides (NPs) of the eel (ANP, atrial natriuretic peptide; CNP, C-type natriuretic peptide; and VNP, ventricular natriuretic peptide) on prolactin (PRL(188) and PRL(177)) and growth hormone (GH) release from the organ-cultured tilapia pituitary were examined. Eel ANG II at concentrations greater than 1 nM stimulated the release of PRL(188) and PRL(177) in a dose-related manner during the first hour of incubation. Significant stimulation by 100 nM ANG II on PRL(177) release was observed until 4h of incubation, and on PRL(188) release until 12 h. No effect of ANG II was seen on GH release. None of the NPs altered the release of PRLs at any time point. On the other hand, eel VNP at concentrations greater than 1 nM stimulated GH release in a dose-related manner after 4 h, and significant stimulation was observed until 48 h. Eel CNP was less effective than eel VNP; significant stimulation of GH release was observed at 1 and 10 nM during 24-48 h of incubation. No significant effect of eel ANP on GH release was seen at any concentration. ANG II had no effect on GH release at any time point. There was no change in mRNA levels of PRLs or GH in the pituitaries incubated with ANG II for 8 h or those incubated with the NPs for 48 h. These results indicate rapid and short-lasting stimulation by ANG II on PRL release and slow and long-lasting stimulation by VNP and CNP on GH release from the tilapia pituitary.
Eckert
Steve M
SM
Department of Zoology and Hawaii Institute of Marine Biology, University of Hawaii, PO Box 1346, Coconut Island, Kaneohe, HI 96744, USA.
Hirano
Tetsuya
T
Leedom
Thomas A
TA
Takei
Yoshio
Y
Gordon Grau
E
E
D000704:Analysis of Variance; D000804:Angiotensin II / Q000502:physiology*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004524:Eels; D013006:Growth Hormone / Q000378:metabolism*; D020098:Natriuretic Peptide, C-Type / Q000502:physiology*; D009924:Organ Culture Techniques; D010902:Pituitary Gland / Q000378:metabolism; D011388:Prolactin / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D013045:Species Specificity; D013223:Statistics as Topic; D017210:Tilapia / Q000378:metabolism*
D012333:RNA, Messenger; D000804:Angiotensin II; D020098:Natriuretic Peptide, C-Type; C068986:ventricular natriuretic peptide, eel; D009320:Atrial Natriuretic Factor; D011388:Prolactin; D013006:Growth Hormone