title: An improved enrichment broth for isolation of Escherichia coli O157, with specific reference to starved cells, from radish sprouts.
abstract: An enrichment broth was developed for the efficient isolation of Escherichia coli O157 from radish sprouts. The broth was buffered peptone water containing 0.5% sodium thioglycolate (STG-BPW), which was designed to allow growth of E. coli O157 in starved and unstarved states. However, this medium suppressed the growth of non-carbohydrate-fermenting obligate aerobes whose colonial appearance on sorbitol MacConkey agar containing cefixime and tellurite (CT-SMAC) resembled that of E. coli O157. Both starved and unstarved cells of E. coli O157 experimentally inoculated into radish sprouts were successfully recovered with STG-BPW enrichment in all cases, most of which showed marked disappearance of E. coli O157-like colonies on CT-SMAC.
authors.affiliation: Kanagawa Prefectural Public Health Laboratory, Asahi-ku, Yokohama 241-0815, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Sata
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Fujisawa
authors.forename: Ro
authors.initials: R
authors.lastname: Osawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Iguchi
authors.forename: Shiro
authors.initials: S
authors.lastname: Yamai
authors.forename: Toshio
authors.initials: T
authors.lastname: Shimada
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D001431:Bacteriological Techniques; D019607:Brassicaceae / Q000382:microbiology*; D002417:Cattle; D003470:Culture Media; D019453:Escherichia coli O157 / Q000254:growth & development* / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans
chemical_list: D003470:Culture Media