title: Detection of Ehrlichia spp. in the blood of wild white-tailed deer in Missouri by PCR assay and serologic analysis.
abstract: Blood samples collected from wild deer in Missouri in November of 2000 and 2001 were positive by PCR assays for Ehrlichia chaffeensis (50 of 217; 23%), Ehrlichia ewingii (44 of 217; 20%), and Anaplasma species (214 of 217; 99%). Nucleotide sequences of selected amplicons from the assay for anaplasma matched sequences of the white-tailed deer agent. Serologic analysis of 112 deer sampled in 2000 showed a very high prevalence of antibodies to E. chaffeensis (97 of 112; 87%) and a low prevalence of antibodies reactive with Anaplasma phagocytophila (2 of 112; 2%).
authors.affiliation: Department of Pediatrics, Washington University School of Medicine and St. Louis Children's Hospital, Washington University School of Medicine, St. Louis, Missouri 63110, USA. arens@kids.wustl.edu
authors.forename: Max Q
authors.initials: MQ
authors.lastname: Arens
authors.forename: Allison M
authors.initials: AM
authors.lastname: Liddell
authors.forename: Gerald
authors.initials: G
authors.lastname: Buening
authors.forename: Monique
authors.initials: M
authors.lastname: Gaudreault-Keener
authors.forename: John W
authors.initials: JW
authors.lastname: Sumner
authors.forename: James A
authors.initials: JA
authors.lastname: Comer
authors.forename: Richard S
authors.initials: RS
authors.lastname: Buller
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Storch
mesh_terms: D000820:Animal Diseases / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001769:Blood / Q000382:microbiology*; D003670:Deer; D004197:Disease Reservoirs; D004536:Ehrlichia / Q000302:isolation & purification*; D016873:Ehrlichiosis / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*; D008923:Missouri / Q000453:epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016036:Seroepidemiologic Studies; D012698:Serologic Tests
chemical_list: D000907:Antibodies, Bacterial