[Transcriptional regulation of gamma- and epsilon-globin genes by basic Krüppel-like factor].
To study the transcription regulatory function of basic Krüppel-like factor (BKLF)on gamma- and epsilon-globin genes, recombinant expression vectors containing the full-length human BKLF gene, and a deletion mutant that lost N-terminal 40 amino acids, were constructed and used, respectively, to transiently transfect COS7 cells in order to assay their reporter activities. Results showed that hBKLF was able to repress the activity of gamma- and epsilon-globin gene promoters, while the antisense nucleic acid specific for hBKLF activated the transcription of these promoters. Deleting 40 amino acids from N-terminus did not influence the transcriptional repression of hBKLF. The stimulatory function of FKLF on gamma- and epsilon-globin gene promoters was also significantly reduced by hBKLF. In addition, BKLF bound the CACCC element in the SHP1 (SH2-containing protein tyrosine phosphatase 1) gene promoter. These results suggest that gamma- and epsilon-globin genes may be transcriptional targets of BKLF, providing evidence for further studies on the role of BKLF in participating the transcriptional regulation of haemocyte-specific genes.
Ma
Xiao-Yun
XY
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Wang
Mang-Ju
MJ
Qu
Xiang-Hu
XH
Xing
Gui-Chun
GC
Zhu
Yun-Ping
YP
He
Fu-Chu
FC
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D019556:COS Cells; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D051741:Kruppel-Like Transcription Factors; D051379:Mice; D009154:Mutation; D015345:Oligonucleotide Probes / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D054645:Protein Phosphatase 1; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D017027:Protein Tyrosine Phosphatases / Q000235:genetics; D017384:Sequence Deletion; D014158:Transcription, Genetic / Q000235:genetics; D014162:Transfection
D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; C501168:KLF3 protein, human; D051741:Kruppel-Like Transcription Factors; D015345:Oligonucleotide Probes; D005914:Globins; D054645:Protein Phosphatase 1; C516724:PTPN11 protein, human; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D017027:Protein Tyrosine Phosphatases; C516725:Ptpn11 protein, mouse