[The effects of quercetin and isorhamnetin on oxidative modification of VLDL induced by Cu2+].
OBJECTIVE
To observe the effects of quercetin(Que) and isorhamnetin (Iso) on the oxidative modification of very low density lipoprotein (VLDL).


METHODS
Human VLDL was prepared by one-step ultracentrifuge and oxidized by Cu2+ in vitro; Que and Iso in different concentrations were added to VLDL before incubation with Cu2+. The MDA, vitamin E contents and the activity of superoxide dismutase(SOD) in ox-VLDL were determined.


RESULTS
Compared with ox-VLDL group, Que and Iso decreased MDA production in a dependent concentration-manner, Que and Iso also elevated Vit E level and SOD activity markedly.


CONCLUSIONS
This experiment indicates that Que and Iso have similar inhibitory effects on the oxidative modification of VLDL.
Li
B
B
Department of Biochemistry, School of Basic Medical Sciences, WCUMS, Chengdu 610041, China.
He
T
T
Li
J
J
Qiu
M
M
D003300:Copper / Q000494:pharmacology*; D044948:Flavonols; D006801:Humans; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D011794:Quercetin / Q000031:analogs & derivatives / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D044948:Flavonols; D008079:Lipoproteins, VLDL; C047368:3-methylquercetin; D008315:Malondialdehyde; D003300:Copper; D011794:Quercetin; D013482:Superoxide Dismutase