Role of Wnt10b and C/EBPalpha in spontaneous adipogenesis of 243 cells.
This report examines the balance of positive and negative adipogenic factors in a line of immortalized 243 embryonic fibroblasts that undergo spontaneous preadipocyte differentiation. Control of adipogenesis reflects the interplay of factors that promote or inhibit expression of C/EBPalpha and PPARgamma. The 243 cells express C/EBPalpha early and at elevated levels compared to 3T3-F442A preadipocytes or adipocytes. Cell clones were derived from the heterogeneous 243 population for ability or inability to differentiate into adipocytes. Wnt10b, a secreted protein that inhibits adipogenesis, is expressed at high levels in cells with low adipogenic potential and is undetectable in preadipocytes that spontaneously differentiate. In contrast, C/EBPalpha is expressed at reduced levels in cells with low adipogenic potential, and is expressed at high levels in preadipocytes that spontaneously differentiate. These data are consistent with a model in which decreased Wnt10b, coupled with increased C/EBPalpha, results in induction of PPARgamma and spontaneous adipogenesis of 243 cells.
Bennett
Christina N
CN
Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI 48109-0622, USA.
Hodge
Christina L
CL
MacDougald
Ormond A
OA
Schwartz
Jessica
J
D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000502:physiology*; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D002999:Clone Cells; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D051153:Wnt Proteins
D022763:CCAAT-Enhancer-Binding Protein-alpha; D011518:Proto-Oncogene Proteins; D051153:Wnt Proteins; C102068:Wnt10b protein, mouse