title: Role of complement C5 and T lymphocytes in pathogenesis of disseminated and mucosal candidiasis in susceptible DBA/2 mice.
abstract: The aims of the study were to compare the pathogenesis of Candida albicans infection in various organs and anatomical regions of C5-deficient (DBA/2) and C5-sufficient (BALB/c) mice, and to evaluate the importance of complement C5 and T lymphocytes as factors that determine host susceptibility or resistance. The kidneys of DBA/2 mice showed higher colonisation and more severe tissue damage than those of BALB/c, but infection at other sites, including oral and vaginal mucosa, was generally similar in the two strains. Passive transfer of C5-sufficient serum into DBA/2 mice decreased the fungal burden in the kidney, and prolonged survival of the reconstituted animals. Depletion of CD4(+) and/or CD8(+) cells did not exacerbate either systemic or mucosal infection when compared to controls, and passive transfer of splenocytes from infected donors caused only a small and transient reduction in numbers of yeasts recovered from the kidney of sub-lethally infected recipients. It is concluded that the acute susceptibility of the kidneys in this mouse strain is due to C5 deficiency expressed on a susceptible genetic background. T lymphocytes, however, appear to have minimal influence on recovery from systemic infection with this isolate of C. albicans.
authors.affiliation: School of Dentistry, Oral Biology and Pathology, University of Queensland, Brisbane, Qld 4072, Australia. r.ashman@uq.edu.au
authors.forename: Robert B
authors.initials: RB
authors.lastname: Ashman
authors.forename: John M
authors.initials: JM
authors.lastname: Papadimitriou
authors.forename: Alma
authors.initials: A
authors.lastname: Fulurija
authors.forename: Karen E
authors.initials: KE
authors.lastname: Drysdale
authors.forename: Camile S
authors.initials: CS
authors.lastname: Farah
authors.forename: Owen
authors.initials: O
authors.lastname: Naidoo
authors.forename: Theo
authors.initials: T
authors.lastname: Gotjamanos
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D002176:Candida albicans / Q000472:pathogenicity; D002177:Candidiasis / Q000276:immunology*; D002180:Candidiasis, Oral / Q000276:immunology / Q000473:pathology; D002181:Candidiasis, Vulvovaginal / Q000276:immunology / Q000473:pathology; D003182:Complement C5 / Q000502:physiology*; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis; D005260:Female; D007113:Immunity, Innate / Q000235:genetics; D007116:Immunization, Passive; D007668:Kidney / Q000473:pathology; D007958:Leukocyte Count; D051379:Mice; D008811:Mice, Inbred DBA; D009504:Neutrophils / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D003182:Complement C5; D016207:Cytokines