title: Interaction of the anaphase-promoting complex/cyclosome and proteasome protein complexes with multiubiquitin chain-binding proteins.
abstract: Fission yeast Rhp23 and Pus1 represent two families of multiubiquitin chain-binding proteins that associate with the proteasome. We show that both proteins bind to different regions of the proteasome subunit Mts4. The binding site for Pus1 was mapped to a cluster of repetitive sequences also found in the proteasome subunit SpRpn2 and the anaphase-promoting complex/cyclosome (APC/C) subunit Cut4. The putative role of Pus1 as a factor involved in allocation of ubiquitinylated substrates for the proteasome is discussed.
authors.affiliation: Medical Research Council Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, United Kingdom.
authors.forename: Michael
authors.initials: M
authors.lastname: Seeger
authors.forename: Rasmus
authors.initials: R
authors.lastname: Hartmann-Petersen
authors.forename: Caroline R M
authors.initials: CR
authors.lastname: Wilkinson
authors.forename: Mairi
authors.initials: M
authors.lastname: Wallace
authors.forename: Itaru
authors.initials: I
authors.lastname: Samejima
authors.forename: Martin S
authors.initials: MS
authors.lastname: Taylor
authors.forename: Colin
authors.initials: C
authors.lastname: Gordon
mesh_terms: D000595:Amino Acid Sequence; D064173:Anaphase-Promoting Complex-Cyclosome; D002352:Carrier Proteins / Q000378:metabolism*; D003546:Cysteine Endopeptidases / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D006836:Hydro-Lyases / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D008025:Ligases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D011485:Protein Binding; D012568:Schizosaccharomyces / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D016415:Sequence Alignment; D043743:Ubiquitin-Protein Ligase Complexes
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; C108670:MTS4 protein, S pombe; D009097:Multienzyme Complexes; C490845:RHP23 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; D006836:Hydro-Lyases; C022479:pseudouridylate synthetase; D008025:Ligases