[In vitro synthesis of immunoglobulins caused by an inactivated Ebola virus].
An in vitro model Ebola infection was used to study the humoral response of human mononuclear cells to stimulation by purified inactivated Ebola virus antigen. Inactivated Ebola virus was cocultivated with human mononuclear cells in the presence or absence of B-cell mitogen LPS E. coli: B5. An increase in the rate of synthesis of immunoglobulins (both IgG and, to a less extent, other classes) was observed. The Ebola virus proteins were suggested to exert no suppression effect on B-cells. The IgM/IgG synthesis was evaluated by EIA in supernatants after 7 days of cultivation. It was concluded that Ebola fever is accompanied by active humoral immune response, which provides a promising basis for further search of the methods of treatment of this disease.
Tuzova
M N
MN
Gaĭdul'
K V
KV
Chepurnov
A A
AA
D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000276:immunology* / Q000494:pharmacology; D002478:Cells, Cultured; D029043:Ebolavirus / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000821:virology*; D013997:Time Factors
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M