title: Enzymological aspects of bioconversion of m-dinitrobenzene.
abstract: Three main enzymes, responsible for bioconversion of 1,3 dinitrobenzene (m-DNB) by Micrococcus colpogenes MCM B 410, were isolated from the sonicated cell mass, grown in presence of m-DNB in a synthetic medium, for 7 days. The soluble proteins were separated by differential precipitation and also separated by native PAGE. Each fraction obtained from both the protocols, was tested for nitro aryl reductase, aryl mono oxygenase and resorcinol 2,3 dioxygenase. The apparent molecular weight of the proteins were nitro aryl reductase (30 kDa), aryl mono oxygenase (110 kDa) and resorcinol 2,3 di oxygenase (65 kDa).
authors.affiliation: Division of Microbial Sciences, Agharkar Research Institute, G.G. Agharkar Road, Pune 411 004, India. sabitadey@hotmail.com
authors.forename: Sabita
authors.initials: S
authors.lastname: Dey
mesh_terms: D001711:Biotransformation; D011232:Chemical Precipitation; D004136:Dinitrobenzenes / Q000378:metabolism*; D008837:Micrococcus / Q000201:enzymology*; D008970:Molecular Weight
chemical_list: D004136:Dinitrobenzenes; C017906:3-dinitrobenzene