Peripheral blood lymphocytes immunophenotype and serum concentration of soluble HLA class I in burn patients.
The level of the soluble form of histocompatibility class I antigens, associated with beta(2)-microglobulin (sHLA-I) has been determined by an ELISA sandwich method in serum from burned patients (n=42) and healthy volunteers (n=30). The sHLA-I level was insignificantly increased in burn patients at the stage of burn shock (1284+/-324U/ml, mean+/-S.E.M.) and after day 28 postburn (1368+/-258U/ml) compared to volunteers (1150+/-90U/ml). At the same time a decrease of sHLA-I levels between 4 and 14 days (638+/-178U/ml) was determined (P<0.05). Increased levels of sHLA, though not significant, were detected in patients with TBSAB >70% in comparison to patients with TBSAB from 30 to 70% during burn shock (1493+/-528 and 1075+/-339U/ml, respectively). Expression of membranous HLA class I antigens (mHLA-I) in peripheral blood lymphocytes (PBLs) was assayed simultaneously by indirect immunofluorescence. The number of CD3(+), CD4(+), CD8(+), CD25(+), CD71(+) and CD26(+) lymphocytes was also evaluated. The expression of mHLA-I in PBLs was increased significantly in patients with TBSAB <70% at early postburn period. Daily monitoring showed that the relative numbers of CD25(+) and CD71(+) lymphocytes in patients varied greatly within short intervals of time during burn shock. The data obtained suggest that mHLA-I expression can reflect postburn lymphocyte activation. The serum content of sHLA-I does not depend on lymphocyte number or activated lymphocyte number in peripheral blood at burned patients.
Lebedev
M Ju
MJ
Laboratory of Immunology, Institute of Traumatology and Orthopaedics, 603155 Verhne Volzhskaya nab. 18, Nizhny Novgorod, Russia. miklgito@mail.ru
Krizhanova
M A
MA
Vilkov
S A
SA
Sholkina
M N
MN
Vyasmina
E S
ES
Baryshnikov
A Ju
AJ
Novikov
V V
VV
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000097:blood*; D002056:Burns / Q000097:blood / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000097:blood* / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011946:Receptors, Antigen / Q000097:blood; D016176:T-Lymphocyte Subsets / Q000276:immunology; D001613:beta 2-Microglobulin / Q000097:blood*
D015703:Antigens, CD; D015395:Histocompatibility Antigens Class I; D011946:Receptors, Antigen; D001613:beta 2-Microglobulin