title: The second largest subunit of RNA polymerase II interacts with and enhances transactivation of androgen receptor.
abstract: AR may communicate with the general transcription machinery on the core promoter to exert its function as a transcriptional modulator. Our previous reports demonstrated that AR interacted with TFIIH and positive transcription elongation factor b (P-TEFb), and that phosphorylation of the carboxy-terminal domain in the largest subunit of RNA polymerase II might play important roles in AR-mediated transcription. These results suggest that AR may modulate gene expression by enhancing the efficiency of transcriptional elongation. Here we further demonstrate that co-expression of the second largest subunit of RNA polymerase II (RPB2) enhances AR transactivation. However, co-expression of the other subunits of RNA polymerase II or TFIIB did not show preferential enhancement of AR-mediated transcription. Furthermore, co-transfection of RPB2 with ER showed little effect on enhancement of ER transactivation. Together, AR may be able to interact with TFIIH, P-TEFb, and RPB2 to enhance transcription from AR target genes, such as prostate specific antigen that may play important roles in the prostate cancer progression.
authors.affiliation: George Whipple Laboratory for Cancer Research, Department of Pathology, Urology, and the Cancer Center, University of Rochester Medical Center, 601 Elmwood Avenue, Box 626, Rochester, NY 14642, USA.
authors.forename: Dong Kun
authors.initials: DK
authors.lastname: Lee
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.forename: Chawnshang
authors.initials: C
authors.lastname: Chang
mesh_terms: D000818:Animals; D002460:Cell Line; D011233:Precipitin Tests; D011485:Protein Binding; D012319:RNA Polymerase II / Q000378:metabolism* / Q000502:physiology; D011944:Receptors, Androgen / Q000235:genetics*; D015533:Transcriptional Activation / Q000502:physiology*
chemical_list: D011944:Receptors, Androgen; D012319:RNA Polymerase II