A copepod life-cycle test and growth model for interpreting the effects of lindane.
A full life-cycle test was performed to measure the effects of lindane (3.2-3,200 microg l(-1)) on the survival, development and reproduction of the freshwater copepod Bryocamptus zschokkei. This copepod survived at relatively high concentrations of lindane compared with other freshwater crustaceans with a 10 day LC50 of 241 microg l(-1) (95% CL of 141-440). 'Equiproportional development', which assumes that each moult stage represents a specific proportion of the total development time, and is not affected by processes that influence metabolism such as temperature and food quality, was used to determine the mode of action of lindane on development in B. zschokkei. Development to adult was significantly longer at 100 microg l(-1) lindane compared with the controls, however, development remained equiproportional regardless of lindane exposure. Increased development times, therefore, are not due to a direct effect of lindane on the moulting process but are due probably to reduced food intake or increased metabolism through the stress imposed by toxicant exposure. Although the survival data suggest that B. zschokkei is relatively tolerant of lindane exposure, reproduction was affected at low lindane concentrations. At 32 microg l(-1) lindane, significantly fewer eggs and viable offspring were produced per female compared with the solvent control. At very low lindane concentrations (3.2 and 10 microg l(-1)), there was a significant increase in the numbers of offspring produced per female compared with the controls and this is interpreted as a hormesis effect. In conclusion, a full life-cycle test demonstrated B. zschokkei is relatively sensitive to lindane compared with other freshwater crustaceans. Incorporating a copepod growth model (equiproportional development) into the life-cycle test design, provided information on the dominant mode of action of the toxicant.
Brown
R J
RJ
School of Biological Sciences (Plymouth Environmental Research Centre), University of Plymouth, Drake Circus, Plymouth, Devon PL4 8AA, UK. rjbrown@plymouth.ac.uk
Rundle
S D
SD
Hutchinson
T H
TH
Williams
T D
TD
Jones
M B
MB
D000818:Animals; D033342:Copepoda / Q000187:drug effects / Q000254:growth & development*; D004305:Dose-Response Relationship, Drug; D005260:Female; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D007928:Lethal Dose 50; D008018:Life Cycle Stages / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity
D007306:Insecticides; D014874:Water Pollutants, Chemical; D001556:Hexachlorocyclohexane