Efficient gene transfer to hepatoblastoma cells through asialoglycoprotein receptor and expression under the control of the cyclin A promoter.
Specific gene delivery into hepatoma cells by liposomes and specific gene expression under the control of the cyclin A promoter were examined in HepG2 cells, a hepatoblastoma cell line that overexpresses cyclin A. A plasmid carrying the luciferase gene under the cyclin A promoter sequence was condensed with poly-L-lysine and encapsulated into anionic asialofetuin-labeled liposomes (AF-liposomes), which were preferentially taken up by hepatocytes through the action of the asialoglycoprotein receptor (AgpR). AF-liposomes delivered plasmids to the hepatoma cells by receptor-mediated endocytosis through the AgpR, and transgene expression could be achieved under the control of the cyclin A promoter. Furthermore, a fusogenic lipid, DOPE, as a liposomal component was required for the enhancement of transfection efficiency of AF-liposomes.
Aramaki
Yukihiko
Y
Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Japan.
Lee
Insugi
I
Arima
Hidetoshi
H
Sakamoto
Takatoshi
T
Magami
Yasushi
Y
Yoshimoto
Takayuki
T
Moriyasu
Fuminori
F
Mizuguchi
Junichiro
J
Koyanagi
Yasuhisa
Y
Nikaido
Toshio
T
Tsuchiya
Seishi
S
D000962:Antimalarials / Q000494:pharmacology; D037263:Asialoglycoprotein Receptor / Q000378:metabolism*; D045744:Cell Line, Tumor; D002738:Chloroquine / Q000494:pharmacology; D019925:Cyclin A / Q000235:genetics*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D018197:Hepatoblastoma / Q000473:pathology; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008156:Luciferases / Q000235:genetics; D008561:Membrane Fusion; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D010957:Plasmids / Q000378:metabolism; D011107:Polylysine / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014162:Transfection / Q000379:methods*
C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D000962:Antimalarials; D037263:Asialoglycoprotein Receptor; D019925:Cyclin A; D008081:Liposomes; D010714:Phosphatidylethanolamines; D011107:Polylysine; D002738:Chloroquine; D008156:Luciferases