title: Extended-spectrum beta-lactamase types in Klebsiella pneumoniae and Escherichia coli in two Greek hospitals.
abstract: Seventy-nine Klebsiella pneumoniae and 124 Escherichia coli clinical strains, isolated consecutively during August-October 2001 in two Greek hospitals, were examined for production of extended-spectrum beta-lactamases (ESBLs). Seventy-one (35%) isolates (46 K. pneumoniae and 25 E. coli) were ESBL-positive by phenotypic methods. Isoelectric focusing of beta-lactamases and PCR assays for bla genes showed that SHV-5-type ESBLs were the most frequent (45 isolates, 22%) followed by CTX-M (24 isolates, 12%) and IBC (three isolates, 1.5%). The latter two ESBL types may have been established recently in this setting.
authors.affiliation: Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Tzelepi
authors.forename: Ch
authors.initials: Ch
authors.lastname: Magana
authors.forename: E
authors.initials: E
authors.lastname: Platsouka
authors.forename: D
authors.initials: D
authors.lastname: Sofianou
authors.forename: O
authors.initials: O
authors.lastname: Paniara
authors.forename: N J
authors.initials: NJ
authors.lastname: Legakis
authors.forename: A C
authors.initials: AC
authors.lastname: Vatopoulos
authors.forename: L S
authors.initials: LS
authors.lastname: Tzouvelekis
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology; D005798:Genes, Bacterial; D006115:Greece / Q000453:epidemiology; D006801:Humans; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D007710:Klebsiella Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases