Inert site in a protein zinc cluster: isotope exchange by high resolution mass spectrometry.
Using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry, we show that one Zn2+ ion in the Zn4 cluster of cyanobacterial metallothionein is inert to exchange with 67Zn2+. We suggest that this is zinc in site A, which together with the surrounding alpha and beta secondary structure forms a zinc-finger fold.
Blindauer
Claudia A
CA
School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, Scotland, U.K.
Polfer
Nick C
NC
Keiper
Stella E
SE
Harrison
Mark D
MD
Robinson
Nigel J
NJ
Langridge-Smith
Pat R R
PR
Sadler
Peter J
PJ
D017357:Cyclotrons; D005583:Fourier Analysis; D013058:Mass Spectrometry / Q000379:methods; D008958:Models, Molecular; D015032:Zinc / Q000737:chemistry*
D015032:Zinc