title: Localization of postresection EGF receptor expression using laser capture microdissection.
abstract: OBJECTIVE
  Epidermal growth factor (EGF) and its receptor (EGFR) are key components in the genesis of adaptation after small bowel resection (SBR). Within intestinal homogenates, EGFR expression is increased after SBR; however, the exact cells responsible for altered EGFR expression are unknown. In this study, laser capture microdissection (LCM) microscopy was used to elucidate the specific cellular compartment(s) responsible for postresection changes in EGFR expression.
  
  
  METHODS
  Male ICR mice underwent a 50% proximal SBR or sham operation. After 3 days, frozen sections were taken from the remnant ileum. Individual cells from villi, crypt, muscularis, and mesenchymal compartments were isolated by LCM. EGFR mRNA expression for each cell compartment was quantified using real-time polymerase chain reaction (PCR).
  
  
  RESULTS
  EGFR expression was increased after SBR within the crypt (2-fold) and muscularis compartments (3-fold). There were no changes detected after SBR in the villus tips or mesenchymal compartments.
  
  
  CONCLUSIONS
  Increased expression of EGFR in crypts directly correlates with the zone of cell proliferation and supports the hypothesis that EGFR signaling is crucial for the mitogenic stimulus for adaptation. The finding of increased EGFR expression in the muscular compartment is novel and may implicate a role for EGFR as a mediator of the muscular hyperplasia seen after massive SBR.
authors.affiliation: Division of Pediatric Surgery, Cincinnati Children's Hospital Medical Center, and the Department of Surgery, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229-3039, USA.
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Knott
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Erwin
authors.forename: Sherri A
authors.initials: SA
authors.lastname: Profitt
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Juno
authors.forename: Brad W
authors.initials: BW
authors.lastname: Warner
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D003199:Computer Systems; D066246:ErbB Receptors / Q000096:biosynthesis* / Q000235:genetics; D005629:Frozen Sections; D016591:Histocytological Preparation Techniques / Q000295:instrumentation; D007082:Ileum / Q000601:surgery* / Q000648:ultrastructure; D007413:Intestinal Mucosa / Q000737:chemistry / Q000648:ultrastructure; D007834:Lasers; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008871:Microvilli / Q000737:chemistry / Q000648:ultrastructure; D009130:Muscle, Smooth / Q000737:chemistry / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000302:isolation & purification; D012778:Short Bowel Syndrome / Q000378:metabolism* / Q000473:pathology
chemical_list: D012333:RNA, Messenger; D066246:ErbB Receptors