title: [Simple analysis of maleic hydrazide in agricultural products by HPLC].
abstract: A simplified HPLC determination method for maleic hydrazide in agricultural products was developed, and commercial agricultural crops were investigated. The homogenate of agricultural products was extracted with water. The crude extract was purified on an ACCUCAT Bond Elut extraction cartridge using water. Maleic hydrazide was analyzed by HPLC with UV detection (303 nm). The HPLC separation was performed on a ZORBAX SB-Aq column with acetonitrile-water-phosphoric acid(5:95:0.01) as the mobile phase. Recoveries of maleic hydrazide from 15 agricultural products fortified at 1.0 and 10 micrograms/g were in the ranges of 92.6-104.9% and 94.2-101.3%, respectively. The limit of detection was 0.5 microgram/g in samples. The proposed method was applied to the determination of 242 commercial vegetables and fruits. Maleic hydrazide was detected in 2 samples of imported onion at the levels of 4.9 and 7.2 micrograms/g.
authors.affiliation: Tokyo Metropolitan Research Laboratory of Public Health: 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nagayama
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takano
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tamura
authors.forename: Yukinari
authors.initials: Y
authors.lastname: Tateishi
authors.forename: Sanae
authors.initials: S
authors.lastname: Tomizawa
authors.forename: Naoko
authors.initials: N
authors.lastname: Kimura
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kitayama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Saito
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018556:Crops, Agricultural / Q000737:chemistry*; D006540:Herbicides / Q000032:analysis*; D008300:Maleic Hydrazide / Q000032:analysis*; D010937:Plant Growth Regulators / Q000032:analysis*
chemical_list: D006540:Herbicides; D010937:Plant Growth Regulators; D008300:Maleic Hydrazide