Electrophoretic analysis of Salmonella typhi and other bacteria.
A comparative study involving SDS-PAGE of Salmonella typhi and other Bacteria was conducted. Protoplasmic antigens of Salmonella typhi. Salmonella paratyphi A, Salmonella typhimurium, Proteus sp, Klebsiellas sp. Pseudomonas aeruginosa, Shigella flexneri, Staphylococcus aureus were separated and compared on SDS-PAGE followed by checking of their cross reactivity by gel diffusion using antisera raised against whole cell and lysates of Salmonella typhi. Lines of identity between Salmonella typhi, Salmonella paratyphi A and Salmonella typhimurium were observed. No lines of identity were seen among Salmonella typhi, Pseudomonas aeruginosa and Sfaphylococcus aureus.
Sood
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Department of Microbiology, University College of Medical Sciences, Guru Teg Bahadur Hospital, Delhi 110095.
Kaur
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D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005779:Immunodiffusion; D012485:Salmonella typhi / Q000276:immunology* / Q000302:isolation & purification*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins