title: Separation of anti-tumor peptides by capillary electrophoresis in organic solvent containing background electrolytes.
abstract: Connections between the calculated and measured electrophoretic mobilities (nu(ep)) determined by capillary electrophoresis as well as connections between the measured and calculated diffusion coefficients of anti-tumor peptides have been investigated in background electrolytes (BGEs) containing different organic solvents (acetonitrile, methanol, ethanol and isopropanol). Comparison of the electrophoretic mobility (nu(ep)) values revealed discrepancies between the measured and calculated values. However, no change in the migration order or selectivity could be expected from the calculated nu(ep) values, variation of both properties was observed applying organic solvents as BGE modifiers. Experimental determination of the diffusion coefficient suggested that the effect of the organic solvents is not restricted to the change of the BGE viscosity. The reason for the discrepancy between the measured and calculated mobility values might be the possible conformation and/or solvation changes of the peptide caused by the different organic solvents.
authors.affiliation: Hungarian Academy of Sciences, Peptidebiochemical Research Group, Semmelweis University, Budapest, Hungary. miki@puskin.sote.hu
authors.forename: Miklós
authors.initials: M
authors.lastname: Idei
authors.forename: Eva
authors.initials: E
authors.lastname: Kiss
authors.forename: Zsófia
authors.initials: Z
authors.lastname: Dobos
authors.forename: Balázs
authors.initials: B
authors.lastname: Hallgas
authors.forename: György
authors.initials: G
authors.lastname: Mészáros
authors.forename: Ferenc
authors.initials: F
authors.lastname: Hollósy
authors.forename: György
authors.initials: G
authors.lastname: Kéri
mesh_terms: D000097:Acetonitriles; D000438:Alcohols; D000970:Antineoplastic Agents / Q000032:analysis* / Q000138:chemical synthesis; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008433:Mathematics; D009842:Oligopeptides / Q000032:analysis* / Q000138:chemical synthesis; D009943:Organophosphorus Compounds / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013004:Somatostatin / Q000031:analogs & derivatives* / Q000138:chemical synthesis
chemical_list: D000097:Acetonitriles; D000438:Alcohols; D000970:Antineoplastic Agents; D004573:Electrolytes; D009842:Oligopeptides; D009943:Organophosphorus Compounds; D000644:Quaternary Ammonium Compounds; D012997:Solvents; C066585:triethylammonium cation; D013004:Somatostatin; C032159:acetonitrile