title: Lens formation in the absence of optic cup in rat embryos irradiated with soft X-ray.
abstract: In order to investigate the effect of soft X-ray irradiation on ocular development, pregnant rats were exposed to a single 12.5 Gy irradiation on embryonic day 9 (ED 9). The embryos obtained by laparotomy on ED 12 and 21 were examined for ocular abnormalities under a binocular stereo-microscope and a light microscope. The ED 12 embryos were stained with osmium tetroxide to facilitate the observation. The stereo-microscopic examination on ED 12 and 21 revealed various types of ocular abnormalities characterized primarily by aplasia or hypoplasia of the optic cup and invaginated lens placode. The light microscopic examination further confirmed these findings histomorphologically, and the hypoplastic abnormalities were classified into three types: (1) hypoplasia of the optic cup and invaginated lens placode, (2) complete malformation of the optic cup and hypoplasia of the invaginated lens placode, and (3) complete malformation of the optic cup and invaginated lens placode. Because the lens was formed in the complete absence of the retina, the development and differentiation of the retina and lens do not seem to be tightly synchronized. Thus, this sequential analysis on ocular abnormalities during the early stage of development supports the notion that the presence of the retina is not always necessary for the development of the lens.
authors.affiliation: Department of Safety Assessment, Development Research Laboratories, Banyu Pharmaceutical Co, Ltd, 810 Nishijo, Menuma, Osato, Saitama, Japan 360-0214. inagkist@banyu.co.jp
authors.forename: Satoru
authors.initials: S
authors.lastname: Inagaki
authors.forename: Tadao
authors.initials: T
authors.lastname: Kotani
mesh_terms: D000016:Abnormalities, Radiation-Induced / Q000196:embryology* / Q000473:pathology; D000818:Animals; D004622:Embryo, Mammalian / Q000528:radiation effects*; D005124:Eye Abnormalities / Q000196:embryology* / Q000473:pathology; D005260:Female; D009993:Osmium Tetroxide; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009993:Osmium Tetroxide