Characterization of pharmaceutical drugs by a modified nonaqueous capillary electrophoresis--mass spectrometry method.
A simple method for the separation and characterization of a group of nine basic compounds, comprising seven tricyclic antidepressant and two bronchodilator drugs, by nonaqueous capillary electrophoresis (NACE) employing ultraviolet and mass spectrometry detection is described. After optimization of the electrophoresis separation conditions, including the compositions of the electrolyte and the organic solvent, a reliable separation of all nine basic analytes was achieved in 80 mM ammonium formate dissolved in a methanol-acetonitrite (80:20 v/v) mixture, having an apparent pH of 8.7. The volatile nonaqueous electrolyte system used with a normal electroosmotic flow polarity also provided an optimal separation condition for the characterization of the analytes by mass spectrometry. When results were compared with reversed-phase gradient and isocratic high-performance liquid chromatography (HPLC) methods, the NACE method provided greater efficiency, achieving baseline resolution for all nine basic compounds in less than 30 min. The NACE method is suitable for use as a routine procedure for the rapid separation and characterization of basic compounds and is a viable alternative to HPLC for the separation of a wide range of pharmaceutical drugs.
Peri-Okonny
Unita L
UL
Bioanalytical Drug Metabolism, Johnson and Johnson Pharmaceutical Research and Development, Raritan, NJ 08869, USA.
Kenndler
Ernst
E
Stubbs
R John
RJ
Guzman
Norberto A
NA
D000929:Antidepressive Agents, Tricyclic / Q000737:chemistry / Q000302:isolation & purification; D001993:Bronchodilator Agents / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D000929:Antidepressive Agents, Tricyclic; D001993:Bronchodilator Agents; D004364:Pharmaceutical Preparations