Kuwanon G: an antibacterial agent from the root bark of Morus alba against oral pathogens.
Kuwanon G was isolated from the ethyl acetate fraction of methanol extract of Morus alba and its structure was elucidated by 13C-NMR, 1H-NMR and FAB-MS. Antibacterial activity of kuwanon G was investigated by the minimum inhibitory concentration (MIC) test and the viable cell count method. MIC of kuwanon G against Streptococcus mutans causing dental caries was determined to be 8.0 microg/ml. The bactericidal test showed that kuwanon G completely inactivated S. mutans at the concentration 20 microg/ml in 1 min. Kuwanon G also significantly inhibited the growth of other cariogenic bacteria such as Streptococcus sobrinus and Streptococcus sanguis, and Porpyromonas gingivalis causing periodontitis. Transmission electron microscopy (TEM) of kuwanon G treated cells demonstrated remarkable morphological damage of the cell wall and condensation of the cytoplasm.
Park
K M
KM
Department of Biotechnology and Bioproducts Research Center, Yonsei University, Seoul 120-749, South Korea.
You
J S
JS
Lee
H Y
HY
Baek
N I
NI
Hwang
J K
JK
D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000648:ultrastructure; D015169:Colony Count, Microbial; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D031625:Morus / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012997:Solvents
D000900:Anti-Bacterial Agents; D005419:Flavonoids; D012997:Solvents; C095194:kuwanon G