title: Cellular profile of cytokine production in a patient with visceral leishmaniasis: gammadelta+ T cells express both type 1 cytokines and interleukin-10.
abstract: The cytokine profile of CD4+, CD8+ T cells, gammadelta+ T cells and natural killer (NK) cells (CD94+CD3-) was studied in a patient with visceral leishmaniasis (VL). The otherwise healthy, human immunodeficiency virus-negative patient acquired the disease in Tuscany, Italy. Diagnosis was made by demonstration of high concentrations of antibodies against Leishmania antigens in serum. Flow cytometry for the detection of intracellular interferon-gamma (IFN-gamma), interleukin (IL)-2, IL-4, IL-6, IL-10, IL-13 and tumour necrosis factor (TNF)-alpha expression in peripheral blood mononuclear cells stimulated with phorbol 12-myristate 13-acetate and ionomycin was performed, followed by treatment with liposomal amphotericin B. CD4+ cells were identified as major cytokine-expressing cells, capable of producing both type 1 and type 2 cytokines. A high frequency of IL-4- and IL-13-expressing CD8+ cells was noted. NK cells and gammadelta+ T cells, thought to be involved in innate host defences against Leishmania, expressed IFN-gamma and TNF-alpha. Ten per cent of gammadelta+ T cells expressed IL-10, predominantly together with IFN-gamma, suggesting additional immune-regulatory roles for this T-cell subset in VL.
authors.affiliation: Department of Internal Medicine I, Division of Infectious Diseases, University of Vienna, Vienna, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Lagler
authors.forename: M
authors.initials: M
authors.lastname: Willheim
authors.forename: F
authors.initials: F
authors.lastname: Traunmüller
authors.forename: K
authors.initials: K
authors.lastname: Wahl
authors.forename: H
authors.initials: H
authors.lastname: Winkler
authors.forename: M
authors.initials: M
authors.lastname: Ramharter
authors.forename: W
authors.initials: W
authors.lastname: Graninger
authors.forename: S
authors.initials: S
authors.lastname: Winkler
mesh_terms: D000666:Amphotericin B / Q000627:therapeutic use; D000981:Antiprotozoal Agents / Q000627:therapeutic use; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis / Q000097:blood*; D005434:Flow Cytometry; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis / Q000097:blood; D007558:Italy; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism; D007898:Leishmaniasis, Visceral / Q000097:blood / Q000188:drug therapy / Q000276:immunology*; D008875:Middle Aged; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D000981:Antiprotozoal Agents; D016207:Cytokines; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016753:Interleukin-10; D000666:Amphotericin B