Natural abundance hydrogen isotope affiliation between the reactants and the products in glucose fermentation with yeast.
In glucose fermentation, the hydrogen source of products such as ethanol and glycerol is the medium and the sugar. The site-specific natural isotope ratios of the products, (D/H)(i), and that of the medium and sugar, (D/H)(k), may be related by a matrix, A, of redistribution coefficients, a(ik), that characterizes the specific genealogies of the hydrogen atoms. (D/H)(i) = [A](D/H)(k), where (D/H)(i) and (D/H)(k) are the column vectors of the isotope ratios of sites i and k that can be measured by (2)H NMR. The complete redistribution matrix was determined in a set of isotope labeling experiments. Thus, we obtained a mathematical model representing the hydrogen isotope affiliation during alcoholic fermentation. It not only provides information about the biochemical reaction mechanism but also can be used to estimate the isotopic data of the products, based on those of the substrate and the medium. The results prove, in a quantitative way, that the metabolites contain isotopic information about the precursor in a biotransformation and can be used to identify its origin. The method established for the study of the hydrogen-transfer mechanism can be applied to other chemical and biochemical reactions.
Pionnier
Sebastien
S
Laboratoire d'Analyse Isotopique et Electrochimique de Métabolismes, CNRS UMR 6006, Université de Nantes, 2, Rue de la Houssinière, 44322 Nantes, France.
Robins
Richard J
RJ
Zhang
Ben-Li
BL
D003903:Deuterium; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D006019:Glycolysis; D009682:Magnetic Resonance Spectroscopy; D008433:Mathematics; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013237:Stereoisomerism
D000431:Ethanol; D003903:Deuterium; D005947:Glucose; D005990:Glycerol