title: Transcriptional network controlled by the trithorax-group gene ash2 in Drosophila melanogaster.
abstract: The transcription factor absent, small, or homeotic discs 2 (ash2) gene is a member of the trithorax group of positive regulators of homeotic genes. Mutant alleles for ash2 are larvalpupal lethals and display imaginal disc and brain abnormalities. The allele used in this study is a true mutant for the trithorax function and lacks the longest transcript present in wild-type flies. In an attempt to identify gene targets of ash2, we have performed an expression analysis by using cDNA microarrays. Genes involved in cell cycle, cell proliferation, and cell adhesion are among these targets, and some of them are validated by functional and expression studies. Even though trithorax proteins act by modulating chromatin structure at particular chromosomal locations, evidence of physical aggregation of ash2-regulated genes has not been found. This work represents the first microarray analysis, to our knowledge, of a trithorax-group gene.
authors.affiliation: Departament de Genètica, Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Spain.
authors.forename: Sergi
authors.initials: S
authors.lastname: Beltran
authors.forename: Enrique
authors.initials: E
authors.lastname: Blanco
authors.forename: Florenci
authors.initials: F
authors.lastname: Serras
authors.forename: Beatriz
authors.initials: B
authors.lastname: Pérez-Villamil
authors.forename: Roderic
authors.initials: R
authors.lastname: Guigó
authors.forename: Spyros
authors.initials: S
authors.lastname: Artavanis-Tsakonas
authors.forename: Montserrat
authors.initials: M
authors.lastname: Corominas
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D017344:Genes, Insect; D005809:Genes, Regulator; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D029721:Drosophila Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; C103604:ash2 protein, Drosophila