Coproantibodies to the enteropathogenic Escherichia coli vaccine candidates BfpA and EspB in breastfed and artificially fed children.
A diagnostic test was developed to discriminate active from past enteropathogenic Escherichia coli (EPEC) infection, which uses the affinity-purified recombinant proteins BfpA (bundle-forming pilus (BFP) structural repeating subunit A) and EspB (pore-forming secreted protein B) as reliable markers of virulence to detect antigen-specific coproantibodies by immunoblot analysis, and verification of active typical EPEC infection by gene-specific (bfpA and espB) PCR amplification using DNA extracted directly from specimens and/or culture-enriched preparations. To begin addressing the potential protective role of anti-EPEC antibodies at early age, the prevalence of IgA coproantibodies to these antigens was determined in either breastfed or artificially fed children <2 years of age hospitalized for watery diarrhea.
de Souza Campos Fernandes
Regina Célia
RC
Hospital Estadual Ferreira Machado, Campos dos Goytacazes-RJ, Brazil.
Quintana Flores
Victor Martin
VM
Sousa de Macedo
Zila
Z
Medina-Acosta
Enrique
E
D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D001903:Bottle Feeding; D001942:Breast Feeding; D002675:Child, Preschool; D004926:Escherichia coli / Q000276:immunology / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000276:immunology / Q000517:prevention & control; D029968:Escherichia coli Proteins / Q000276:immunology* / Q000378:metabolism; D022361:Escherichia coli Vaccines / Q000276:immunology* / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005260:Female; D036781:Fimbriae Proteins / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D015995:Prevalence; D003969:Vipoma / Q000378:metabolism
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; C104901:BfpA protein, E coli; C087863:EaeB protein, E coli; D029968:Escherichia coli Proteins; D022361:Escherichia coli Vaccines; D036781:Fimbriae Proteins