title: Low content of protein S29 in ribosomes of human lung cancer cell line a549: detected by two-dimensional electrophoresis.
abstract: This study revealed that the content of protein S29 in ribosomes of cancer cell line A549 was distinctly low (equivalent to about 30% of that of 2BS). The conclusion was acquired based on the ratios of spot volume of ribosomal protein S29 to that of several other ribosomal proteins (S29/L37a, S29/L38, S29/S27 and S29/S28) in the same gel plate. The possible biological roles of ribosomal protein S29 in malignant transformation and translation regulation are briefly discussed.
authors.affiliation: State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China.
authors.forename: Zhi-Dong
authors.initials: ZD
authors.lastname: Zhou
authors.forename: Lei
authors.initials: L
authors.lastname: Bao
authors.forename: Ding-Gan
authors.initials: DG
authors.lastname: Liu
authors.forename: Min-Qian
authors.initials: MQ
authors.lastname: Li
authors.forename: Yin-Zi
authors.initials: YZ
authors.lastname: Ge
authors.forename: Ying-Ling
authors.initials: YL
authors.lastname: Huang
authors.forename: Wang-Yi
authors.initials: WY
authors.lastname: Liu
mesh_terms: D015152:Blotting, Northern; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism; D008168:Lung / Q000166:cytology; D008175:Lung Neoplasms / Q000378:metabolism*; D012313:RNA / Q000302:isolation & purification; D012269:Ribosomal Proteins / Q000032:analysis* / Q000302:isolation & purification; D016622:Silver Staining / Q000379:methods
chemical_list: D012269:Ribosomal Proteins; C079855:ribosomal protein S29; D012313:RNA