Recombinant Lapemis hardwickii phospholipase A2 inhibits rat vascular smooth muscle cell proliferation stimulated by advanced glycation end products.
OBJECTIVE
To observe the effects of recombinant Lapemis hardwickii phospholipase A(2) (rPLA(2)) on vascular smooth muscle cell (VSMC) proliferation stimulated by advanced glycation end products (AGEs).


METHODS
Rat aortic VSMCs were cultured in vitro and stimulated with AGEs of different concentrations prior to co-treatment with rPLA(2) and AGEs. The response of the VSMCs to these treatments was observed in comparison with that of the control group. Non-radioactive MTS/PES assay was adopted for the quantification of the cell proliferation ratio.


RESULTS
Compared with the control group, AGEs significantly stimulated VSMCs proliferation (P < 0.05), but even with AGEs stimulation, rPLA(2) inhibited the growth of VSMCs (0.753+/-0.098 vs 1.100+/-0.226, P < 0.05).


CONCLUSIONS
rPLA(2) can significantly inhibit the proliferation of VSMCs stimulated by AGEs.
Ouyang
Ping
P
Department of Cardiology, Nanfang Hospital, First Military Medical University, Guangzhou 510515 China.
Xu
Ding-li
DL
Yang
Wen-li
WL
Lai
Wen-yan
WY
Xu
An-long
AL
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D004347:Drug Interactions; D017815:Elapidae; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D010741:Phospholipases A / Q000494:pharmacology*; D054467:Phospholipases A2; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology
D017127:Glycation End Products, Advanced; D011994:Recombinant Proteins; D010741:Phospholipases A; D054467:Phospholipases A2