Proteomics of breast cancer: outcomes and prospects.
Breast cancer is a major public health problem. The identification of new markers to differentiate neoplastic from the normal cells, more thorough understanding of different stages of the pathology, as well as the definition of new therapeutic targets, are all of critical importance. With the completion of human genome sequencing and the introduction of mass spectrometry, combined with protein identification via advanced bioinformatics, proteomics has emerged as a valuable tool for the discovery of new molecular markers. New methods in functional proteomics have also been developed to study the intracellular signaling pathways that underline the development of breast cancer. As illustrated with the examples of fibroblast growth factor-2 and H19, an oncogenic, noncoding mRNA, proteomics have become a powerful approach for deciphering the complex signaling circuitry involved in tumor growth. Breast cancer proteomics have already identified proteins of potential clinical interest (such as the molecular chaperone 14-3-3 sigma) and technological innovations in large scale/high throughput analysis are now ushering in new prospects.
El Yazidi-Belkoura
Ikram
I
Laboratoire de Biologie du developpement UPRES-EA 1033, Universite des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.
Adriaenssens
Eric
E
Vercoutter-Edouart
Anne-Sophie
AS
Lemoine
Jérôme
J
Nurcombe
Victor
V
Hondermarck
Hubert
H
D001943:Breast Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D002455:Cell Division; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis; D006801:Humans; D008954:Models, Biological; D020543:Proteome; D062085:RNA, Long Noncoding; D012333:RNA, Messenger / Q000378:metabolism; D022661:RNA, Untranslated / Q000096:biosynthesis; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
C094335:H19 long non-coding RNA; D020543:Proteome; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D022661:RNA, Untranslated; D016222:Fibroblast Growth Factor 2