Semisynthesis of proteins by expressed protein ligation.
Expressed protein ligation (EPL) is a protein engineering approach that allows recombinant and synthetic polypeptides to be chemoselectively and regioselectively joined together. The approach makes the primary structure of most proteins accessible to the tools of synthetic organic chemistry, enabling the covalent structure of proteins to be modified in an unprecedented fashion. The ability to incorporate noncoded amino acids, biophysical probes, and stable isotopes into specific locations within proteins provides research tools to peer into the inner workings of these molecules. In this review I discuss the development of this technology, its broad application to biological systems, and its possible role in the area of proteomics.
Muir
Tom W
TW
Laboratory of Synthetic Protein Chemistry, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA. muirt@mail.rockefeller.edu
D000596:Amino Acids / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D008958:Models, Molecular; D010446:Peptide Fragments / Q000737:chemistry; D014176:Protein Biosynthesis; D015202:Protein Engineering / Q000379:methods* / Q000639:trends; D011499:Protein Processing, Post-Translational; D019154:Protein Splicing; D011506:Proteins / Q000138:chemical synthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000138:chemical synthesis / Q000235:genetics
D000596:Amino Acids; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D011506:Proteins; D011994:Recombinant Proteins