title: Enhancement of growth by expression of poplar cellulase in Arabidopsis thaliana.
abstract: To study the role of cellulose and cellulase in plant growth, we expressed poplar cellulase (PaPopCel1) constitutively in Arabidopsis thaliana. Expression increased the size of the rosettes due to increased cell size. The change in growth was accompanied by changes in biomechanical properties due to cell wall structure indicative of decrease in xyloglucan cross-linked with cellulose microfibrils by chemical analysis and nuclear magnetic resonance (NMR) spectra. The result supports the concept that the paracrystalline sites of cellulose microfibrils are attacked by poplar cellulase to loosen xyloglucan intercalation and this irreversible wall modification promotes the enlargement of plant cells.
authors.affiliation: Wood Research Institute, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Yong Woo
authors.initials: YW
authors.lastname: Park
authors.forename: Rumi
authors.initials: R
authors.lastname: Tominaga
authors.forename: Junji
authors.initials: J
authors.lastname: Sugiyama
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Furuta
authors.forename: Eiichi
authors.initials: E
authors.lastname: Tanimoto
authors.forename: Masahiro
authors.initials: M
authors.lastname: Samejima
authors.forename: Fukumi
authors.initials: F
authors.lastname: Sakai
authors.forename: Takahisa
authors.initials: T
authors.lastname: Hayashi
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D002455:Cell Division; D002473:Cell Wall / Q000737:chemistry / Q000502:physiology; D002480:Cellulase / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D018546:Hypocotyl / Q000166:cytology / Q000254:growth & development; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000166:cytology / Q000254:growth & development; D030821:Plants, Genetically Modified; D032107:Populus / Q000201:enzymology* / Q000235:genetics; D019076:Transgenes / Q000235:genetics
chemical_list: D002480:Cellulase