Tropane alkaloids in auxin-independent root cultures of Physochlaina physaloides.
Adventitious and hairy root cultures of Physochlaina physaloides were established. These roots grew well and produced high amounts of tropane alkaloids (particularly hyoscyamine and 6 beta-hydroxyhyoscyamine) in auxin-free culture medium. The effects of basal media and temperature on the growth and alkaloid production of these roots were investigated. Both root cultures produced highest amount of tropane alkaloids in B5 medium though the optimum temperature for hairy roots were lower than that for adventitious roots.
Shimomura
Koichiro
K
Faculty of Life Science, Toyo University, 1-1-1 Izumino, Itakura-machi, Oura-gun, Gunma, 374-0193 Japan.
Hirose
Makoto
M
Natori
Shinsaku
S
Satake
Motoyoshi
M
Yoshimatsu
Kayo
K
Ishimaru
Kanji
K
D003470:Culture Media; D007210:Indoleacetic Acids; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D019657:Solanaceae / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature; D014326:Tropanes / Q000378:metabolism*
D003470:Culture Media; D007210:Indoleacetic Acids; D014326:Tropanes