title: Substrate specificity of NovM: implications for novobiocin biosynthesis and glycorandomization.
abstract: [reaction: see text] In an effort to expand the scope of natural product in vitro glycorandomization (IVG), the substrate specificity of NovM was investigated. A test of four aglycon analogues and over 40 nucleotide sugars revealed NovM has a surprisingly stringent substrate specificity and provided only three new "unnatural" natural products. On the basis of the determined substrate specificity, an alternative to the sugar nucleotide biosynthetic dogma and a cautionary note for the general applicability of IVG are introduced.
authors.affiliation: Laboratory for Biosynthetic Chemistry, Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison, Wisconsin 53705, USA.
authors.forename: Christoph
authors.initials: C
authors.lastname: Albermann
authors.forename: Aileen
authors.initials: A
authors.lastname: Soriano
authors.forename: Jiqing
authors.initials: J
authors.lastname: Jiang
authors.forename: Heidi
authors.initials: H
authors.lastname: Vollmer
authors.forename: John B
authors.initials: JB
authors.lastname: Biggins
authors.forename: William A
authors.initials: WA
authors.lastname: Barton
authors.forename: Jacob
authors.initials: J
authors.lastname: Lesniak
authors.forename: Dimitar B
authors.initials: DB
authors.lastname: Nikolov
authors.forename: Jon S
authors.initials: JS
authors.lastname: Thorson
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D006027:Glycosides / Q000737:chemistry; D009675:Novobiocin / Q000096:biosynthesis*; D013302:Streptomyces; D013379:Substrate Specificity
chemical_list: D000900:Anti-Bacterial Agents; D006027:Glycosides; D009675:Novobiocin