Increased frequency of CD3/8/56-positive umbilical cord blood T lymphocytes after allo-priming in vitro.
Umbilical cord blood (UCB) or adult peripheral blood mononuclear cells (PBMC) were repeatedly stimulated by HLA-mismatched allogeneic Epstein-Barr virus (EBV)-transformed cell lines, and the bulk responders or single-cloned cells were immunophenotypically analyzed by flow cytometry. One month after the allo-stimulation, not in PBMC but in UCB, the proportion of CD3/8/56 triple positive T lymphocytes significantly increased. Furthermore, UCB clones exhibited those unique CD3/8/56 markers at an extremely higher frequency than PB clones. They showed as much strong killing activity against allo-stimulators as conventional PB CD56-negative, CD8 T-cell clones, whereas they did not kill the other target, Raji cells. UCB CD3/8/56 T cell clones produced a smaller amount of interferon-gamma compared with PB CD4 or PB CD8 T cell clones. We concluded that CD8 T cells coexpressed with CD56 marker expanded after allo-priming in vitro and would become one of the graft-versus-host (GVH) effectors after UCB transplantation.
Hagihara
M
M
Department of Hematology and Oncology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan. masaoha@is.icc.u-tokai.ac.jp
Chargui
J
J
Gansuvd
B
B
Inoue
H
H
Hotta
T
T
Kato
S
S
D017252:CD3 Complex / Q000032:analysis*; D019002:CD56 Antigen / Q000032:analysis*; D016827:CD8 Antigens / Q000032:analysis*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002461:Cell Line, Transformed; D036101:Cord Blood Stem Cell Transplantation; D003602:Cytotoxicity, Immunologic; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D006680:HLA Antigens / Q000276:immunology; D004854:Herpesvirus 4, Human; D006801:Humans; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D007959:Lymphocyte Culture Test, Mixed; D036102:Peripheral Blood Stem Cell Transplantation; D013601:T-Lymphocytes / Q000276:immunology*
D017252:CD3 Complex; D019002:CD56 Antigen; D016827:CD8 Antigens; D006680:HLA Antigens; D015847:Interleukin-4; D007371:Interferon-gamma