Reciprocal variations of nNOS and HSP90 are associated with fasting in gastrointestinal tract of the piglet.
The effects of fasting on neuronal NO synthase (nNOS), Heme oxygenase 2 (HO-2), and heat shock protein 90 (HSP90) was determined by immunoblotting in the stomach, duodenum, mid-jejunum, distal ileum, and proximal colon of 28-day-old piglets. nNOS expression was drastically reduced in all the gastrointestinal areas studied while HO-2 was not changed. Concomitant with the nNOS decrease, elevated expressions of HSP90 were observed in these different areas. These results are discussed in terms of the regulation relationship between NOS and HSP90 and the possible protective effect of the heat shock protein and the potential application in digestive pathologies.
Grongnet
Jean-François
JF
Ecole Nationale Supérieure Agronomique de Rennes, 65, rue de Saint-Brieuc, CS 84 215, 35 042 Rennes Cedex, France.
David
Jean-Claude
JC
D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D004064:Digestive System / Q000201:enzymology; D004068:Digestive System Physiological Phenomena; D005215:Fasting / Q000009:adverse effects*; D005260:Female; D018841:HSP90 Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism*; D006419:Heme Oxygenase (Decyclizing) / Q000032:analysis / Q000378:metabolism*; D015151:Immunoblotting; D008297:Male; D023421:Models, Animal; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000378:metabolism*; D018384:Oxidative Stress; D011336:Probability; D012016:Reference Values; D012680:Sensitivity and Specificity
D018841:HSP90 Heat-Shock Proteins; D019001:Nitric Oxide Synthase; D006419:Heme Oxygenase (Decyclizing); C105245:heme oxygenase-2