title: Apoptosis and lens vesicle development.
abstract: OBJECTIVE
  To study the development of the rat lens vesicle in relation to apoptosis.
  
  
  METHODS
  Fetuses of Wistar Kyoto rats were removed by laparotomy on day 10-15 of gestation. Some fetuses were fixed in 2% paraformaldehyde and embedded in paraffin for a TUNEL technique examination of DNA fragmentation. Macrophages were stained immunohistochemically with antibody. Some fetuses were fixed in 4% glutaraldehyde and 1% osmic acid and embedded in Luveak 812, then examined with a transmission electron microscope (TEM).
  
  
  RESULTS
  On day 11 of gestation (E11) before the start of lens invagination, apoptotic changes were noted in the cells between the surface ectoderm and optic vesicle, with the appearance of phagocytic cells. Apoptotic cells were present at the junction of the surface ectoderm and the lens placode, in the ventral and dorsal thirds of the lens placode and in the outer layer of the optic vesicle in the same axes on E12. Apoptotic changes appeared in the lens stalk, surface ectoderm and the anterior lens epithelium on E12.5. The lens vesicle was detached completely from the surface ectoderm by E13 and some cells had the typical characteristics of macrophages in the extracellular space between the surface ectoderm and the anterior lens epithelium. Apoptotic changes were confirmed by the TUNEL method, and macrophages were stained immunohistochemically.
  
  
  CONCLUSIONS
  Apoptosis may have a major role during the whole process of lens vesicle development. Apoptosis may eliminate the cells between the surface ectoderm and the optic vesicle, help trigger invagination and facilitate separation from the ectoderm. Apoptosis might aid in the bowing of the optic vesicle during lens invagination.
authors.affiliation: Department of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Sakamoto, Nagasaki, Japan.
authors.forename: Y H
authors.initials: YH
authors.lastname: Mohamed
authors.forename: T
authors.initials: T
authors.lastname: Amemiya
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D004247:DNA / Q000032:analysis; D004475:Ectoderm / Q000502:physiology / Q000648:ultrastructure; D005314:Embryonic and Fetal Development / Q000502:physiology*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D007908:Lens, Crystalline / Q000196:embryology* / Q000648:ultrastructure; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D008854:Microscopy, Electron; D009024:Morphogenesis; D051381:Rats; D011921:Rats, Inbred WKY
chemical_list: D004247:DNA