title: Divergence of reiterated sequences in a series of genital isolates of herpes simplex virus type 1 from individual patients.
abstract: Both serotypes of herpes simplex virus (HSV), HSV-1 and HSV-2, are aetiological agents of genital herpes, although genital herpes caused by HSV-1 recurs less frequently. The HSV-1 genome contains a number of short, tandemly repeated sequences, and some reiterated sequences can serve as sensitive markers for the differentiation of HSV-1 strains. In the present study, variation in reiterations (assumed to be due to different copy numbers of tandemly repeated sequences) was examined in HSV-1 isolates from genital lesions from the same individual. Six sets (three primary-recurrence sets and three multiple-recurrence sets) of HSV-1 isolates were analysed: the primary-recurrence set consisted of two isolates (one isolated at a primary episode and the other at a recurrent episode) from the same individual; the multiple-recurrence set consisted of plural isolates from different episodes of recurrence in the same individual. Variations in length of the major DNA fragment, containing reiteration I (within the a sequence) and/or reiteration IV (within introns of genes US1 and US12), were detected between isolates of each multiple-recurrence set, but not of the primary-recurrence set. Thus, HSV-1 isolates of multiple-recurrence sets are assumed to have diverged more widely within each set than those of primary-recurrence sets, probably because of more rounds of virus DNA replication. This divergence of reiterations seems to indicate a forward step in the division of HSV-1 from a common ancestor into different lineages.
authors.affiliation: Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Umene
authors.affiliation: Department of Obstetrics and Gynecology, Mizonokuchi Hospital, Teikyo University, Kawasaki 213-8507, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kawana
mesh_terms: D015331:Cohort Studies; D004279:DNA, Viral / Q000737:chemistry; D005260:Female; D014644:Genetic Variation; D006558:Herpes Genitalis / Q000473:pathology / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D007438:Introns; D005810:Multigene Family; D012150:Polymorphism, Restriction Fragment Length; D012008:Recurrence; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D004279:DNA, Viral; C057294:ICP22 protein, human herpesvirus 1; C105843:ICP47 protein, Herpes simplex virus; D017874:Immediate-Early Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C078424:EUS1 protein, Equine herpesvirus 1