title: [Studies of oligosaccharide specificity of perch roe fucolectin, using gold labeled neoglycoproteins].
abstract: The specificity of perch (Perca fluviatilis) roe fucolectin was studied using the protein dot blot technique, followed by detection with colloidal gold-labeled neoglycoproteins bearing human milk polysaccharides. The strongest binding was noted with the H type 1 pentasaccharide lacto-N-fucopentaose (LNFP I, Fuc alpha 1-2 Gal beta 1-3 GlcNAc beta 1-3 Gal beta 1-4Glc); the interaction with the H type 6 trisaccharide 2'-fucosyllactose (2-FL, Fuc alpha 1-2 Gal beta 1-4 Glc) was weaker. Binding of the perch lectin to the Lewis antigens (associated with tumors and embryonic tissues) was also studied. It was found that the lectin weakly interacted with the hexasaccharide lacto-N-difucohexaose I, Fuc alpha 1-2 Gal beta 1-3[Fuc alpha 1-4]GlcNac beta 1-3 Gal beta 1-4 Glc), but not with Lea, Lec, Lex antigens. Thus, perch roe lectin exhibited pronounced differences in carbohydrate specificity from other fucolectins--a feature that may be used in structural studies and isolation of fucose-containing glycoconjugates.
authors.affiliation: Nesmeyanov Institute of Organoelemental Compounds, Russian Academy of Sciences, Moscow, 119991 Russia. piskarev@ineos.as.ru
authors.forename: V E
authors.initials: VE
authors.lastname: Piskarev
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Praĭzel'
authors.forename: R P
authors.initials: RP
authors.lastname: Evstigneeva
authors.forename: I A
authors.initials: IA
authors.lastname: Iamskov
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D005643:Fucose / Q000032:analysis*; D006023:Glycoproteins; D017979:Gold Colloid; D007118:Immunoassay; D037102:Lectins / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000276:immunology; D010063:Ovum / Q000737:chemistry*; D010471:Perches; D013045:Species Specificity; D013194:Staining and Labeling; D014312:Trisaccharides / Q000276:immunology
chemical_list: D006023:Glycoproteins; D017979:Gold Colloid; D037102:Lectins; D009844:Oligosaccharides; D014312:Trisaccharides; C027112:fucose-binding lectin; C058457:Aurodye; C018991:lacto-N-difucohexaose I; D005643:Fucose; C031420:2'-fucosyllactose