title: Clinical use of polyethylene glycols as marker substances and determination in urine by liquid chromatography.
abstract: Adulteration of samples is a serious problem in the analysis of drugs of abuse. One of the most frequent methods is substitution of urines by "clean" urines to gain false-negative results in laboratory tests for drugs of abuse. One way to approach this problem may be to label the patient's urine with marker substances which are given orally prior to the delivery of urine. This concept is based on methods for determining malabsorption in pediatric medicine. We report a protocol for evaluating low-molecular-mass polyethylene glycols as enteral labelling marker substances. For monitoring renal excretion of the ingested polyethylene glycols we have developed and optimised an isocratic reversed-phase high-performance liquid chromatographic method with automatic sample cleanup by column switching in the back-flush technique and with RI detection. The chromatographic procedure is simple, reliable and rapid, allowing a high sample throughput for routine screening.
authors.affiliation: Kliniken der Stadt Köln, Zentrallabor, Ostmerheimerstrasse 200, 51109 Köln, Germany.
authors.forename: Gisela
authors.initials: G
authors.lastname: Gauchel
authors.forename: Bernd
authors.initials: B
authors.lastname: Huppertz
authors.forename: Heribert
authors.initials: H
authors.lastname: Feiertag
authors.forename: Ruprecht
authors.initials: R
authors.lastname: Keller
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011092:Polyethylene Glycols / Q000032:analysis*; D012680:Sensitivity and Specificity; D014556:Urine
chemical_list: D011092:Polyethylene Glycols