title: The effects of sevoflurane and desflurane in vitro on platelet-leukocyte adhesion in whole blood.
abstract: The interaction between platelets and leukocytes plays an important role in inflammatory and thrombotic processes. We investigated whether the volatile anaesthetics sevoflurane and desflurane alter the formation of platelet-leukocyte aggregates and the expression of P-selectin on platelets. Whole blood was incubated with 1 and 2 minimum alveolar concentration (MAC) sevoflurane or desflurane. Unstimulated and adenosine diphosphate, or thrombin receptor agonist peptide-6-stimulated samples were stained with fluorochrome-conjugated antibodies. The formation of platelet-leukocyte conjugates and the expression of P-selectin on platelets were measured using flow cytometry. Sevoflurane was found to enhance the binding of platelets to lymphocytes, neutrophils and monocytes, it also increased the expression of P-selectin on platelets especially in the stimulated samples. Desflurane decreased the percentage of lymphocyte-platelet, neutrophil-platelet and monocyte-platelet conjugates principally in unstimulated samples. The results show that these two volatile anaesthetics have differing effects on the formation of platelet-leukocyte conjugates in vitro. Sevoflurane also enhanced the expression of P-selectin on platelets.
authors.affiliation: Department of Anaesthesiology, Rheinisch-Westfälische Technische Hochschule Aachen, Pauwelsstrasse 30, 52074 Aachen, Germany. nhorn@ukaachen.de
authors.forename: N A
authors.initials: NA
authors.lastname: Horn
authors.forename: L
authors.initials: L
authors.lastname: de Rossi
authors.forename: T
authors.initials: T
authors.lastname: Robitzsch
authors.forename: K E
authors.initials: KE
authors.lastname: Hecker
authors.forename: G
authors.initials: G
authors.lastname: Hutschenreuter
authors.forename: R
authors.initials: R
authors.lastname: Rossaint
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D000077335:Desflurane; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007530:Isoflurane / Q000031:analogs & derivatives* / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects* / Q000502:physiology; D008738:Methyl Ethers / Q000494:pharmacology*; D019007:P-Selectin / Q000097:blood; D015539:Platelet Activation / Q000187:drug effects; D010973:Platelet Adhesiveness / Q000187:drug effects*; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism; D000077149:Sevoflurane
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D019007:P-Selectin; D019038:Platelet Glycoprotein GPIb-IX Complex; D000077149:Sevoflurane; D000077335:Desflurane; D007530:Isoflurane