title: Proteolytic activity of a yeast cell wall lytic Arthrobacter species.
abstract: OBJECTIVE
  To investigate properties of the proteolytic activity of a yeast cell wall lytic soil bacterium identified as an Arthrobacter species.
  
  
  RESULTS
  The organism was grown at pH 7.5 and 30 degrees C in shake flasks on media with different complex subtrates. Highest proteolytic activity assayed with azocaseine was detected in media with wheat gluten. In addition, l-leucine, l-alanine exopeptidase activity and esterase activity were found. The proteolytic activity showed stability up to pH 12, with a maximum at pH 11. The temperature optimum was at 55 degrees C, but there was a loss in enzyme activity of 50% within 2 h. The proteolytic activity was inhibited by 3,4-dichloroisocumarin, whereas there was little or no effect with EDTA, pepstatin A or E64.
  
  
  CONCLUSIONS
  The proteolytic activity is highly alkaline stable. The formation of the enzyme can be induced by media with high protein content.
authors.affiliation: Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria. bernhard.adamitsch@tuwien.ac.at
authors.forename: B F
authors.initials: BF
authors.lastname: Adamitsch
authors.forename: F
authors.initials: F
authors.lastname: Karner
authors.forename: W
authors.initials: W
authors.lastname: Hampel
mesh_terms: D001173:Arthrobacter / Q000254:growth & development / Q000378:metabolism*; D002364:Caseins / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D003470:Culture Media; D010450:Endopeptidases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008666:Metalloendopeptidases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012988:Soil Microbiology; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D002364:Caseins; D003470:Culture Media; C026018:azocasein; D010450:Endopeptidases; D008666:Metalloendopeptidases; C030895:caseinase