Schistosoma mansoni-infected mice produce antibodies that cross-react with plant, insect, and mammalian glycoproteins and recognize the truncated biantennaryN-glycan Man3GlcNAc2-R.
To reveal the role of cross-reactive carbohydrate determinants in the host immune response in helminth infections and allergenicity, we developed monoclonal antibodies (mAbs) that recognize glycan epitopes present on glycoconjugates from both helminths and plants. An IgM mAb (100-4G11-A) was selected from a panel of anti-glycan mAbs generated from Schistosoma-infected or immunized mice because it recognized both a plant glycoprotein horseradish peroxidase and phospholipase A2 from honeybee venom. On further characterization, it was shown that mAb 100-4G11-A recognizes the truncated biantennary N-glycan Man3GlcNAc2-R. Immunocytochemical analysis and immunoblotting with this mAb demonstrated that Man3GlcNAc2-R structures occur on many glycoproteins of schistosomes and other invertebrates. Remarkably, Man3GlcNAc2-R is also expressed on a restricted number of vertebrate glycoproteins. Our data indicate that this truncated N-glycan is immunogenic in mice during the course of infection. Nevertheless, no elevated antibody levels against this glycan epitope could be detected in sera of individuals infected with Schistosoma mansoni.
van Remoortere
Alexandra
A
Department of Molecular Cell Biology, Glycoimmunology Group, Vu University Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam, the Netherlands.
Bank
Christine M C
CM
Nyame
A Kwame
AK
Cummings
Richard D
RD
Deelder
Andre M
AM
van Die
Irma
I
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000918:Antibody Specificity; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003429:Cross Reactions / Q000276:immunology*; D006023:Glycoproteins / Q000737:chemistry* / Q000276:immunology*; D006735:Horseradish Peroxidase / Q000276:immunology; D007313:Insecta / Q000276:immunology; D008322:Mammals / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000276:immunology; D054467:Phospholipases A2; D010944:Plants / Q000276:immunology; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology*; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology*
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D011134:Polysaccharides; D006735:Horseradish Peroxidase; D010741:Phospholipases A; D054467:Phospholipases A2