Epithelial sodium channel (ENaC) subunit mRNA and protein expression in rats with puromycin aminonucleoside-induced nephrotic syndrome.
In experimental nephrotic syndrome, urinary sodium excretion is decreased during the early phase of the disease. The molecular mechanism(s) leading to salt retention has not been completely elucidated. The rate-limiting constituent of collecting duct sodium transport is the epithelial sodium channel (ENaC). We examined the abundance of ENaC subunit mRNAs and proteins in puromycin aminonucleoside (PAN)-induced nephrotic syndrome. The time courses of urinary sodium excretion, plasma aldosterone concentration and proteinuria were studied in male Sprague-Dawley rats treated with a single dose of either PAN or vehicle. The relative amounts of alphaENaC, betaENaC and gammaENaC mRNAs were determined in kidneys from these rats by real-time quantitative TaqMan PCR, and the amounts of proteins by Western blot. The kinetics of urinary sodium excretion and the appearance of proteinuria were comparable with those reported previously. Sodium retention occurred on days 2, 3 and 6 after PAN injection. A significant up-regulation of alphaENaC and betaENaC mRNA abundance on days 1 and 2 preceded sodium retention on days 2 and 3. Conversely, down-regulation of alphaENaC, betaENaC and gammaENaC mRNA expression on day 3 occurred in the presence of high aldosterone concentrations, and was followed by a return of sodium excretion to control values. The amounts of alphaENaC, betaENaC and gammaENaC proteins were not increased during PAN-induced sodium retention. In conclusion, ENaC mRNA expression, especially alphaENaC, is increased in the very early phase of the experimental model of PAN-induced nephrotic syndrome in rats, but appears to escape from the regulation by aldosterone after day 3.
Audigé
A
A
Division of Nephrology and Hypertension, Inselspital, University of Berne, Freiburgstrasse 3, 3010 Berne, Switzerland.
Yu
Z R
ZR
Frey
B M
BM
Uehlinger
D E
DE
Frey
F J
FJ
Vogt
B
B
D000450:Aldosterone / Q000097:blood; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D053503:Epithelial Sodium Channels; D015870:Gene Expression; D007668:Kidney / Q000737:chemistry*; D008297:Male; D009404:Nephrotic Syndrome / Q000378:metabolism*; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics; D021122:Protein Subunits / Q000032:analysis / Q000235:genetics; D011692:Puromycin Aminonucleoside; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012964:Sodium / Q000652:urine; D015222:Sodium Channels
D053503:Epithelial Sodium Channels; D020033:Protein Isoforms; D021122:Protein Subunits; D012333:RNA, Messenger; D015222:Sodium Channels; D000450:Aldosterone; D011692:Puromycin Aminonucleoside; D012964:Sodium