Studies on the long-term thermal stability of stationary phases in subcritical water chromatography.
The long-term thermal stability of five commercially available reversed-phase columns has been evaluated under subcritical water conditions (100 and 150 degrees C). The five columns included Zorbax RX-C8, Nucleosil C18 AB, Hypersil BDS C18, PRP-1 (poly(styrene-divinylbenzene)), and ZirChrom-PS (polystyrene) columns. Retention factors, plate numbers, and peak areas were monitored over a prolonged period of time. Comparing the three silica-based columns, the Zorbax RX-C, column was the most stable followed by the Nucleosil C18, AB column. The Hypersil BDS C18 column was the least stable under subcritical water conditions. The ZirChrom-PS column was stable at 100 degrees C for at least 7600 column volumes. Of all five columns, the polymeric PRP-1 column was the most stable under subcritical water conditions.
He
Ping
P
Department of Chemistry, College of Arts and Sciences, East Carolina University, Greenville, NC 27858-4353, USA.
Yang
Yu
Y
D002853:Chromatography, Liquid / Q000295:instrumentation*; D015203:Reproducibility of Results; D013696:Temperature