Sulphated endothelial ligands for L-selectin in lymphocyte homing and inflammation.
Lymphocytes from the blood home to secondary lymphoid tissues through a process of tethering, rolling, firm adhesion and transmigration. Tethering and rolling of lymphocytes is mediated by the interaction of L-selectin on lymphocytes with sulphated ligands expressed by the specialized endothelial cells of high endothelial venules (HEVs). The sulphate-dependent monoclonal antibody MECA79 stains HEVs in peripheral lymph nodes and recognizes the complex of HEV ligands for L-selectin termed peripheral node addressin. High endothelial cell GlcNAc-6-sulphotransferase/L-selectin ligand sulphotransferase is a HEV-expressed sulphotransferase that contributes to the formation of the MECA79 epitope and L-selectin ligands on lymph node HEVs. MECA79-reactive vessels are also common at sites of chronic inflammation, suggesting mechanistic parallels between lymphocyte homing and inflammatory trafficking.
van Zante
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Department of Anatomy, Program in Immunology, University of California, San Francisco, CA 94143-0452, U.S.A.
Rosen
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D000818:Animals; D002634:Chemotaxis, Leukocyte; D042783:Endothelial Cells / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000473:pathology*; D019041:L-Selectin / Q000378:metabolism / Q000502:physiology*; D008024:Ligands; D008214:Lymphocytes / Q000166:cytology; D013431:Sulfates / Q000378:metabolism; D015238:Sulfotransferases; D000097811:Carbohydrate Sulfotransferases
D019041:L-Selectin; D008024:Ligands; D013431:Sulfates; D015238:Sulfotransferases; D000097811:Carbohydrate Sulfotransferases