title: High levels of hyaluronan production by a malignant lymphoma cell line with primary effusion lymphoma immunophenotype OHK.
abstract: We investigated whether hyaluronan (HA) was produced by a malignant lymphoma cell line with a primary effusion lymphoma immunophenotype, OHK. OHK secreted high levels of HA into the culture supernatant, and reverse transcription polymerase chain reaction showed mRNA for the HA synthases 2 and 3. HA binding protein-reactive HA was present in the plasma membrane and in the cytoplasm. The inoculation of OHK into severe combined immunodeficiency disease mice produced marked viscid ascites with concentrated HA and a vascular-rich mesenteric solid tumour. These results show that HA is produced and secreted by the malignant lymphoma cell line OHK.
authors.affiliation: Department of Pathology, Osaka Medical College, Osaka, Japan. pa2020@art.osaka-med.ac.jp
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kuwabara
authors.forename: Masahiko
authors.initials: M
authors.lastname: Yoneda
authors.forename: Masami
authors.initials: M
authors.lastname: Nagai
authors.forename: Hajime
authors.initials: H
authors.lastname: Nishio
authors.forename: Taizo
authors.initials: T
authors.lastname: Tasaka
authors.forename: Koichi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mori
mesh_terms: D000818:Animals; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism*; D016130:Immunophenotyping; D008223:Lymphoma / Q000276:immunology / Q000503:physiopathology*; D051379:Mice; D016513:Mice, SCID; D009374:Neoplasms, Experimental; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1; D006820:Hyaluronic Acid