title: Increases in insulin-like growth factor-1 level and peroxidative damage after gestational ethanol exposure in rats.
abstract: Ethanol exposure during pregnancy elicits profound detrimental developmental and behavioral effects such as reduced levels of insulin-like growth factor-1 (IGF-1) in the fetus. However, few reports have addressed its impact on postpartum dams. This study was designed to examine the influence of gestational ethanol exposure on postpartum maternal organ oxidative damage and IGF-1 level. Pregnant female rats were pair-fed from Day 2 of gestation until labor with control or ethanol (6.36% (v/v)) liquid diets and were sacrificed 6 weeks after parturition (ethanol withdrawn after parturition). There was no difference in body weight during or after the gestational period between the control and ethanol groups. Litter size was significantly less for ethanol-fed dams. One-week postnatal pup survival was significantly lower in the ethanol-fed (57.1%) than the control (97.8%) group. Liver and kidney tissue IGF-1 levels and mRNA were elevated in the ethanol-fed mothers, accompanied by hepatic but not renal oxidative damage, indicated by profound lipid peroxidation (measured by malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE)) and protein carbonyl formation. The levels of glutathione (GSH), glutathione disulfide (GSSG) and GSH/GSSG ratios in liver and kidney were not different between the ethanol-fed and control dams. Collectively, these data suggest that gestational ethanol exposure may lead to postpartum oxidative organ damage and a possible compensatory increase in organ IGF-1 levels.
authors.affiliation: Department of Pharmacology, Physiology and Therapeutics, University of North Dakota School of Medicine, Grand Forks, ND 58203, USA. jren@uwyo.edu
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.forename: Z K Fariba
authors.initials: ZK
authors.lastname: Roughead
authors.forename: Loren E
authors.initials: LE
authors.lastname: Wold
authors.forename: Faye L
authors.initials: FL
authors.lastname: Norby
authors.forename: Sharlene
authors.initials: S
authors.lastname: Rakoczy
authors.forename: Renee L
authors.initials: RL
authors.lastname: Mabey
authors.forename: Holly M
authors.initials: HM
authors.lastname: Brown-Borg
mesh_terms: D000818:Animals; D000431:Ethanol / Q000008:administration & dosage / Q000009:adverse effects*; D005260:Female; D005865:Gestational Age; D005978:Glutathione / Q000032:analysis; D019803:Glutathione Disulfide / Q000032:analysis; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects; D018811:Maternal Exposure / Q000009:adverse effects*; D023421:Models, Animal; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000431:Ethanol; D007334:Insulin-Like Growth Factor I; D005978:Glutathione; D019803:Glutathione Disulfide