Genetic transformation of silver birch (Betula pendula) by particle bombardment.
We used in vitro callus and shoot cultures as target material for genetic transformation of silver birch (Betula pendula Roth) by particle bombardment. Cultivation of in vitro shoot cultures before particle bombardment and a long selection period, combined with a high concentration of selective agent after bombardment, led to the production of transformed plantlets that were stable, and no escapes were found among the tree lines produced. Clonal variation in transformation efficiency was found in transient expression of the beta-glucuronidase gene in callus cultures and in plantlets transformed by stable integration of the ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) and neomycin phosphotransferase (npt2) genes.
Valjakka
Maarit
M
Finnish Forest Research Institute, Punkaharju Research Station, FIN-58450 Punkaharju, Finland.
Aronen
Tuija
T
Kangasjärvi
Jaakko
J
Vapaavuori
Elina
E
Häggman
Hely
H