The presence and functions of muscarinic receptors in human T cells: the involvement in IL-2 and IL-2 receptor system.
The existence and functions of muscarinic acetylcholine (mACh) receptors in human T lymphocytes were investigated. RT-PCR analysis demonstrated the presence of M(1) and M(2) subtypes of mACh receptors in human T lymphocytes. Pretreatment with oxotremorine-M (Oxo-M) caused the increase in phytohemagglutinin-induced IL-2 production. Since 4-DAMP suppressed Oxo-M-caused enhancement in IL-2 production, M(1) receptors seem to be involved in the enhancement of the production. Oxo-M stimulated IL-2 receptor mRNA expression and DNA synthesis. Our results suggest that muscarinic receptors, perhaps M(1) receptors are involved in the enhancement of TCR-induced IL-2 production and IL-2 receptor expression in human T lymphocytes. Thus muscarinic receptors positively modulate cell growth in human T lymphocytes by the autocrine mechanism through enhancing expression of both IL-2 and the receptors.
Nomura
Jun
J
Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.
Hosoi
Toru
T
Okuma
Yasunobu
Y
Nomura
Yasuyuki
Y
D000109:Acetylcholine / Q000494:pharmacology; D015152:Blotting, Northern; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D015342:DNA Probes; D018076:DNA, Complementary / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007376:Interleukin-2 / Q000502:physiology*; D019169:Jurkat Cells; D018721:Muscarinic Agonists / Q000494:pharmacology; D010095:Oxotremorine / Q000494:pharmacology; D010276:Parasympatholytics / Q000494:pharmacology; D010890:Pirenzepine / Q000031:analogs & derivatives* / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D015375:Receptors, Interleukin-2 / Q000502:physiology*; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
D015342:DNA Probes; D018076:DNA, Complementary; D007376:Interleukin-2; D018721:Muscarinic Agonists; D010276:Parasympatholytics; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2; D011976:Receptors, Muscarinic; D010890:Pirenzepine; D010095:Oxotremorine; D004247:DNA; D000109:Acetylcholine; C048593:otenzepad