title: Serum free and phospholipid-bound choline decrease after surgery and methylprednisolone administration in dogs.
abstract: We designed this study to determine whether serum free and phospholipid-bound choline concentrations change after surgery or methylprednisolone treatment in dogs and rats. In dogs, serum free and phospholipid-bound choline concentrations were decreased by 29% and 17% immediately after abdominal-pelvic surgery under xylasine+ketamine anesthesia, respectively, and both remained low for 24 h. Serum cortisol was elevated after surgery. The elevation in serum cortisol was inversely correlated with the decreases in free (r=-0.737; P<0.001) and phospholipid-bound (r=-0.771; P<0.001) choline concentrations. After methyprednisolone administration (5-20 mg/kg) free and phospholipid-bound choline concentrations decreased in a dose- and time-dependent manner. In rats, either surgery or methylprednisolone failed to alter serum free choline concentrations while phospholipid-bound choline decreased after surgery. These data show that the decrease in serum choline after surgery results from the increase in circulating glucocorticoids.
authors.affiliation: Department of Biochemistry, Merkez Laboratory, Uludag University Medical School, 16059 Gorukle, Bursa, Turkey. yesim@uludag.edu.tr
authors.forename: Yesim Ozarda
authors.initials: YO
authors.lastname: Ilcol
authors.forename: Zeki
authors.initials: Z
authors.lastname: Yilmaz
authors.forename: Ismail H
authors.initials: IH
authors.lastname: Ulus
mesh_terms: D000818:Animals; D002794:Choline / Q000097:blood*; D004285:Dogs; D005260:Female; D007044:Hysterectomy / Q000009:adverse effects; D008775:Methylprednisolone / Q000009:adverse effects / Q000494:pharmacology*; D010052:Ovariectomy / Q000009:adverse effects; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010743:Phospholipids; D002794:Choline; D008775:Methylprednisolone