title: Huntingtin-interacting protein-1-related protein of rat (rHIP1R) is localized in the postsynaptic regions.
abstract: We cloned a rHIP1R (GenBank Accession No., AB005052) encoding a Sla2/huntingtin-interacting protein (HIP1) family protein from a rat brain cDNA library. Localization of rHIP1R was investigated in the rat brain using an antibody specific to the HIP1R antibody. The rHIP1R protein was enriched in the synaptic plasma membrane fraction along with huntingtin, a synaptic protein and a causal protein for Huntington's disease. The electron microscopic examination revealed that HIP1R was localized at postsynaptic spines. Localization of HIP1R in the small vesicular structures in the spine, possible sites of vesicular transport of synaptic proteins, together with the structure-based analysis, suggested a role of HIP1R for vesicle trafficking through interaction with F-actin and working together with huntingtin and HIP1 at the synaptic sites.
authors.affiliation: Department of Neuroplasticity, Research Center on Aging and Adaptation, University School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Okano
authors.forename: Nobuteru
authors.initials: N
authors.lastname: Usuda
authors.forename: Kenichi
authors.initials: K
authors.lastname: Furihata
authors.forename: Kouzoh
authors.initials: K
authors.lastname: Nakayama
authors.forename: Qing
authors.initials: Q
authors.lastname: Bao Tian
authors.forename: Takashi
authors.initials: T
authors.lastname: Okamoto
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry / Q000235:genetics; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D004268:DNA-Binding Proteins; D015723:Gene Library; D006801:Humans; D008297:Male; D051379:Mice; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D017386:Sequence Homology, Amino Acid; D013569:Synapses / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D033921:Vesicular Transport Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C073368:HIP1 protein, human; C489537:HIP1R protein, human; C489536:Hip1 protein, mouse; C489534:Hip1r protein, mouse; C489535:Hip1r protein, rat; D008840:Microfilament Proteins; D012333:RNA, Messenger; D033921:Vesicular Transport Proteins