Role of reactive oxygen species in organophosphate insecticide phosalone toxicity in erythrocytes in vitro.
Reactive oxygen species (ROS) caused by organophosphates may be involved in the toxicity of various pesticides. Therefore, in this study we aimed to investigate how an organophosphate insecticide, phosalone, affects lipid peroxidation (LPO) and the antioxidant defence system in vitro. For this purpose, the effects of various doses of phosalone on LPO and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in erythrocytes were studied. Each phosalone dose was incubated with a previously prepared erythrocyte sample at +4 degrees C for 0, 60 and 180 min. After incubation, the levels of malondialdehyde (MDA) and the activities of SOD, GSH-Px and CAT were determined. Phosalone caused an increase in MDA formation and a decrease in the activities of SOD, GSH-Px and CAT. However, these effects were seen only at extremely high concentrations of phosalone and these concentrations were in the lethal range. Therefore, we suggest that ROS may not involve in the toxic effects of the pesticidal use of phosalone in low concentrations.
Altuntas
I
I
Suleyman Demirel University, School of Medicine, Department of Biochemistry and Clinical Biochemistry, Isparta, Turkey. irfanaltuntas@hotmail.com
Delibas
N
N
Doguc
D K
DK
Ozmen
S
S
Gultekin
F
F
D000328:Adult; D002374:Catalase / Q000037:antagonists & inhibitors; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors; D006801:Humans; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000097:blood / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors
D002800:Cholinesterase Inhibitors; D009946:Organothiophosphorus Compounds; D017382:Reactive Oxygen Species; C003117:phosalone; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase