title: An improved selective isolation medium for the recovery of Listeria monocytogenes from smoked fish.
abstract: OBJECTIVE
  The aim of this study was to improve the selective isolation of Listeria monocytogenes from smoked haddock fillets.
  
  
  RESULTS
  Listeria selective agar (LSA)--Oxford formulation was supplemented with 25 microg x ml(-1) of colistin sulphate and 30 microg x ml(-1) of nalidixic acid. Inocula from four smoked haddock fillets produced colonies (approx. 2-13 bacteria x g(-1)), identified as L. monocytogenes, on LSA supplemented with antimicrobial compounds (MLSA). Moreover, there was only negligible evidence of bacteria which were not L. monocytogenes on MLSA. In contrast, LSA supported dense bacterial growth, which was not equated with L. monocytogenes.
  
  
  CONCLUSIONS
  The modified medium permitted the recovery of L. monocytogenes from smoked haddock fillets and reduced the growth of contaminating bacteria.
authors.affiliation: School of Life Sciences, Heriot-Watt University, Riccarton, Edinburgh, UK.
authors.forename: A A N
authors.initials: AA
authors.lastname: Neamatallah
authors.forename: S J
authors.initials: SJ
authors.lastname: Dewar
authors.forename: B
authors.initials: B
authors.lastname: Austin
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D003470:Culture Media; D005399:Fishes / Q000382:microbiology*; D005516:Food Microbiology; D005524:Food Technology / Q000379:methods; D006801:Humans; D008089:Listeria monocytogenes / Q000302:isolation & purification* / Q000472:pathogenicity; D008088:Listeriosis / Q000209:etiology
chemical_list: D003470:Culture Media