title: Differential protein expression in the cytosol fraction of an MCF-7 breast cancer cell line selected for resistance toward melphalan.
abstract: Analysis of differential protein expression in the cytosol of melphalan-resistant and -susceptible MCF-7 cell lines has been carried out using a combination of two-dimensional gel electrophoresis, mass spectrometry, and bioinformatics. Comparison of multiple digitized gel arrays detected several spots as candidates for differentially expressed proteins in melphalan-resistant MCF-7 cells. The up-regulated proteins included retinoic acid binding protein II, an isoform of the macrophage migration inhibition factor, and other unidentified proteins. The down-regulated proteins included calreticulin, cyclophin A, and an isoform of the 27 kD heat shock protein. Correlation of the differential expression of some of the proteins with acquired resistance of MCF7 cells to melphalan is discussed.
authors.affiliation: Department of Chemistry and Biochemistry, University of Maryland, College Park Maryland 20742, USA. hathout@wam.umd.edu
authors.forename: Yetrib
authors.initials: Y
authors.lastname: Hathout
authors.forename: Kelly
authors.initials: K
authors.lastname: Riordan
authors.forename: Marion
authors.initials: M
authors.lastname: Gehrmann
authors.forename: Catherine
authors.initials: C
authors.lastname: Fenselau
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000378:metabolism*; D037282:Calreticulin / Q000096:biosynthesis; D019295:Computational Biology; D021984:Cyclophilin A / Q000096:biosynthesis; D003600:Cytosol / Q000378:metabolism; D019992:Databases as Topic; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm; D015180:Electrophoresis, Gel, Two-Dimensional; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D006801:Humans; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis; D013058:Mass Spectrometry; D008558:Melphalan / Q000494:pharmacology*; D014176:Protein Biosynthesis; D020033:Protein Isoforms; D011506:Proteins / Q000737:chemistry; D018168:Receptors, Retinoic Acid / Q000096:biosynthesis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014407:Tumor Cells, Cultured
chemical_list: D018906:Antineoplastic Agents, Alkylating; D037282:Calreticulin; D006360:Heat-Shock Proteins; D008263:Macrophage Migration-Inhibitory Factors; D020033:Protein Isoforms; D011506:Proteins; D018168:Receptors, Retinoic Acid; C082928:retinoic acid binding protein II, cellular; D021984:Cyclophilin A; D008558:Melphalan