title: Molecular typing of exfoliative toxin-producing Staphylococcus aureus strains involved in epidermolytic infections.
abstract: Genotyping of sixteen exfoliative toxin-producing (ET-positive) strains of Staphylococcus aureus isolated in maternity units of two distant hospitals was accomplished by PFGE, ribotyping, PCR ribotyping, and prophage carriage. Three strains secreted combined ETA + ETB, and the remaining produced ETA and enterotoxin C, or TSST-1. The comparison of various genomic profiles resulted in the identification of nine genotypes. The presence of one prevailing genotype was demonstrated in each hospital. Evidence was given that the outbreak-related ET-positive strains causing the skin disease pemphigus neonatorum disseminated in both the hospitals did not originate from a single source or a common ancestor.
authors.affiliation: Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Brno, Czech Republic. vladkar@sci.muni.cz
authors.forename: Vladislava
authors.initials: V
authors.lastname: Růzicková
authors.forename: Roman
authors.initials: R
authors.lastname: Pantůcek
authors.forename: Petr
authors.initials: P
authors.lastname: Petrás
authors.forename: Jirí
authors.initials: J
authors.lastname: Doskar
authors.forename: Ivo
authors.initials: I
authors.lastname: Sedlácek
authors.forename: Stanislav
authors.initials: S
authors.lastname: Rosypal
mesh_terms: D000328:Adult; D015373:Bacterial Typing Techniques; D001768:Blister / Q000453:epidemiology / Q000382:microbiology; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005083:Exfoliatins / Q000096:biosynthesis*; D005260:Female; D005838:Genotype; D006771:Hospitals, Maternity; D006801:Humans; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D039002:Prophages; D021521:Ribotyping; D013206:Staphylococcal Scalded Skin Syndrome / Q000453:epidemiology / Q000382:microbiology; D013207:Staphylococcal Skin Infections / Q000453:epidemiology* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000378:metabolism / Q000821:virology
chemical_list: D005083:Exfoliatins