title: Prevalence of Borrelia burgdorferi species and identification of Borrelia valaisiana in questing Ixodes ricinus in the Lyon region of France as determined by polymerase chain reaction-restriction fragment length polymorphism.
abstract: Many cases of Lyme borreliosis have been reported over the years in the region of Lyon, France. The identification and prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus were investigated by polymerase chain reaction (PCR) of the flagellin gene and restriction fragment length polymorphism (RFLP) analysis. Questing Ixodes ricinus larvae, nymphs and adults were collected by the flagging method from deciduous forests in four areas in the Lyon region of France between October 1994 and September 1995 and in June 1998. The overall prevalence of Borrelia burgdorferi sensu lato was 13.2% (91/688). No significant differences in prevalence were observed between the different stages and sex of the ixodids or between collection areas. The majority of infections were simple infections (82.4%; 75/91), most of which were due to Borrelia afzelii (41.4%), while coinfections (12.1%) were predominantly (54.5%) a combination of Borrelia valaisiana and Borrelia garinii. No tick was infected with more than two borrelial species, nor was Borrelia lusitaniae identified. The Borrelia valaisiana species was detected for the first time in France, confirming its widespread presence in Europe. This study confirms that the surroundings of Lyon are risk areas for contracting Lyme disease and that no particular clinical manifestations predominate due to the heterogeneous distribution of Borrelia genospecies. Moreover, the polymerase chain reaction restriction fragment length polymorphism analysis is a rapid and easy method for genotyping of Borrelia species.
authors.affiliation: Pharmacie, Hôpital Debrousse, 29 rue Soeur Bouvier, 69322, Lyon Cedex 05, France, tquessada@club-internet.fr
authors.forename: T
authors.initials: T
authors.lastname: Quessada
authors.forename: F
authors.initials: F
authors.lastname: Martial-Convert
authors.forename: S
authors.initials: S
authors.lastname: Arnaud
authors.forename: H
authors.initials: H
authors.lastname: Leudet De La Vallee
authors.forename: B
authors.initials: B
authors.lastname: Gilot
authors.forename: J
authors.initials: J
authors.lastname: Pichot
mesh_terms: D000818:Animals; D001179:Arthropod Vectors / Q000469:parasitology*; D001898:Borrelia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D025065:Borrelia burgdorferi / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005602:France / Q000453:epidemiology; D018884:Ixodes / Q000469:parasitology*; D008018:Life Cycle Stages; D008193:Lyme Disease / Q000453:epidemiology / Q000635:transmission*; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid