title: [Characteristics of clinical isolates of Mycobacterium tuberculosis using molecular biological methods].
abstract: A total of 230 clinically drug-resistant and 3 drug-sensitive isolates of M. tuberculosis obtained from patients in Tula and Tula region in 1998-2001 were studied. The RFLP-IS6110 genotyping showed that 52 (30.2%) of isolates had unique patterns, and 120 (69.8%) of them were grouped to form 16 clusters. 95 (55.2%) and 53 (30.8%) of isolates were attributed to groups A1 and W (Beijing), respectively. Double mycobacterium cultures were detected in 4.1% of isolates. 2 to 4 clinical isolates were obtained from each of 55 patients during 1.5 years. A replacement of mycobacterium isolates was registered in the course of treatment in 12 (21.8%) patients. No replacement of clinical isolates occurred in 43 (78.2%) patients during the whole follow-up period. Repeatedly obtained isolates acquired the determinants of drug-resistance in 5 (11.6%) patients. Changes in the quantity of IS6110 elements were registered only in 1.05% of isolates during a 3-year follow-up.
authors.affiliation: State Research Center for Applied Microbiology, Obolensk, Moscow Region.
authors.forename: I G
authors.initials: IG
authors.lastname: Shemiakin
authors.forename: V N
authors.initials: VN
authors.lastname: Stepanshina
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Ivanov
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Lipin
authors.forename: O V
authors.initials: OV
authors.lastname: Korobova
authors.forename: V A
authors.initials: VA
authors.lastname: Anisimova
mesh_terms: D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length