title: Global approaches to protein-protein interactions.
abstract: The availability of complete, annotated genome sequences for a variety of eukaryotic organisms has paved the way for a paradigm shift in biomedical research from the 'one gene-one hypothesis' approach to more global, systematic strategies that analyse genes or proteins on a genome- and proteome-wide scale. One daunting task in the post-genome era is to determine how the complement of expressed cellular proteins - the proteome - is organised into functional, higher-order networks, by mapping all constitutive and dynamic protein-protein interactions. Traditionally, reductionist approaches have typically focused on a few, selected gene products and their interactions in a particular physiological context. In contrast, more holistic strategies aim at understanding complex biological systems, for example global protein-protein interaction networks on a cellular or organismal level. Several large-scale proteomics technologies have been developed to generate comprehensive, cellular protein-protein interaction maps.
authors.affiliation: Cellzome AG, Meyerhofstrasse 1, 69117, Heidelberg, Germany. gerard.drewes@cellzome.com
authors.forename: Gerard
authors.initials: G
authors.lastname: Drewes
authors.forename: Tewis
authors.initials: T
authors.lastname: Bouwmeester
mesh_terms: D005057:Eukaryotic Cells / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D008954:Models, Biological; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods* / Q000639:trends; D015003:Yeasts / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D011506:Proteins