Flow cytometry in human reproductive biology.
Flow cytometry (FC) is an analytical cytology technique which has been extensively used for decades. It has many advantages compared with other similar methods for the study of cell biology, even on a molecular basis. FC allows the cell-by-cell analysis of many optical or immunological features in the same sample, at the same time, and at a rate of thousands of cells per second, generating immense quantities of data and thus providing almost limitless information which is statistically robust due to the number of units studied. The aim of this review is to describe the contribution of FC to the study of physiological and pathological processes related to human reproduction, and to discuss how this technique has been used in research, as well as its clinical applications in this field. We have used some practical examples selected from the most relevant studies within a wide range of investigations published in the literature, and we have also drawn on our own experience of using flow cytometry to study different phenomena related to reproduction. It is conclued that FC is a useful instrument for basic investigation of gynecological issues, as well as for the study of male reproductive characteristics, either in research applications or directly for clinical diagnostic purposes. Future development of these techniques will permit further advances both in our knowledge and in the improvement of assisted reproduction techniques.
Garrido
N
N
Instituto Valenciano de Infertilidad; Department of Pediatrics, Obstetrics and Gynecology, Valencia University School of Medicine, Valencia, Spain.
Meseguer
M
M
Remohi
J
J
Pellicer
A
A
Simón
C
C
D020101:Acrosome Reaction; D000988:Antispermatogenic Agents; D001323:Autoantibodies / Q000032:analysis; D004247:DNA / Q000032:analysis; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D005831:Genital Diseases, Female; D006176:Gynecology; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012098:Reproduction; D027724:Reproductive Techniques, Assisted; D012661:Semen / Q000737:chemistry; D013091:Spermatogenesis; D013094:Spermatozoa / Q000276:immunology
D000988:Antispermatogenic Agents; D001323:Autoantibodies; D008565:Membrane Proteins; D011506:Proteins; D004247:DNA