title: Determination of protease inhibitors using liquid chromatography-tandem mass spectrometry.
abstract: A method for the analysis of six protease inhibitors and one metabolite has been developed and validated. Amprenavir, ritonavir, saquinavir, lopinavir, indinavir, nelfinavir, and an active metabolite of nelfinavir (M8) are quantitated using reversed-phase liquid chromatography coupled to tandem mass spectrometry, equipped with an electrospray ionization source (ESI-LC-MS-MS). The validation data presented here shows that the method allows the rugged analysis of these species from one aliquot. The evolution of complex drug interactions assessments and the clinical use of therapeutic drug monitoring for these antiretrovirals will be a potential immediate application of this method.
authors.affiliation: Pharmacotherapy Research Center, Core Analytical Laboratory, Department of Pharmacy Practice, University at Buffalo, State University of New York, Room 317 Hochstetter Hall, Buffalo, NY 14260-1200, USA. emorse@acsu.buffalo.edu
authors.forename: Valerie A
authors.initials: VA
authors.lastname: Frerichs
authors.forename: Robin
authors.initials: R
authors.lastname: DiFrancesco
authors.forename: Gene D
authors.initials: GD
authors.lastname: Morse
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D017320:HIV Protease Inhibitors / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D017320:HIV Protease Inhibitors