Immune response and protection elicited by DNA immunisation against Taenia cysticercosis.
The study evaluated DNA vaccination in Taenia solium cysticercosis prevention by using cDNA of an antigen (cC1) from T. solium metacestode. pcDNA3-cC1 DNA vaccine was constructed by inserting the cDNA into the eukaryotic expression plasmid pcDNA3. Positive expression of the pcDNA3-cC1 product was confirmed by its transfection into COS7 cell and enzyme-linked immunoabsorbent assay using serum of pigs infected with T. solium metacestode. Immunisation of BALB/c mice with three injections of pcDNA3-cC1 induced antigen-specific immune responses of the Th1 phenotype. Inoculation of new-born pigs induced protection against challenge with T. solium by 73.3% reduction of the metacestode number. Antibodies elicited by DNA immunisation with pcDNA3-cC1 specifically reacted with native cC1 protein, which was mainly restricted to the cyst wall of T. solium metacestode. Positive apoptosis signals were also detected in the cyst wall cells of metacestode slices from pigs immunised with pcDNA3-cC1 by TUNEL staining method. Those suggested that apoptosis played a role in protecting pigs immunised with pcDNA3-cC1 nucleic acid vaccine from pathogen challenge.
Wang
Qing-min
QM
Department of Medical Genetics, The Second Military Medical University, Xiang'Yin Road 800, 200433, Shanghai, China.
Sun
Shu-han
SH
Hu
Zhen-lin
ZL
Wu
Dan
D
Wang
Zhong-chuan
ZC
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D003551:Cysticercosis / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013552:Swine; D041201:Taenia solium / Q000235:genetics* / Q000276:immunology*; D019444:Vaccines, DNA / Q000235:genetics* / Q000276:immunology / Q000627:therapeutic use*
D019444:Vaccines, DNA