Optimization of microwave-assisted extraction (MAP) for ginseng components by response surface methodology.
Response surface methodology (RSM) was applied to predict optimum conditions for microwave-assisted extraction-a MAP technology-of saponin components from ginseng roots. A central composite design was used to monitor the effect of ethanol concentration (30-90%, X(1)) and extraction time (30-270 s, X(2)) on dependent variables, such as total extract yield (Y(1)), crude saponin content (Y(2)), and saponin ratio (Y(3)), under atmospheric pressure conditions when focused microwaves were applied at an emission frequency of 2450 MHz. In MAP under pre-established conditions, correlation coefficients (R (2)) of the models for total extract yield and crude saponin were 0.9841 (p < 0.001) and 0.9704 (p < 0.01). Optimum extraction conditions were predicted for each variable as 52.6% ethanol and 224.7 s in extract yield and as 77.3% ethanol and 295.1 s in crude saponins, respectively. Estimated maximum values at predicted optimum conditions were in good agreement with experimental values.
Kwon
Joong-Ho
JH
Department of Food Science and Technology, Kyungpook National University, Taegu 702-701, Korea.
Bélanger
Jacqueline M R
JM
Paré
J R Jocelyn
JR
D000431:Ethanol; D008872:Microwaves; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification*
D010936:Plant Extracts; D012503:Saponins; D000431:Ethanol