title: Stable octameric structure of recombinant hemoglobin alpha(2)beta(2)83 Gly-->Cys.
abstract: We have engineered a recombinant hemoglobin (rHb betaG83C) based on the variant Hb Ta-Li, which oligomerizes through intertetramer disulfide bonds. Size exclusion chromatography and electrospray ionization mass spectrometry show that the rHb betaG83C assembles into an oligomeric structure the size of a dimer of tetramers. The oligomer has carbon monoxide-binding properties similar to those of natural human hemoglobin. Unlike HbA, the oligomer does not participate in dimer exchange. The CO kinetics, auto-oxidation rate, and gel filtration experiments on the oligomeric betaG83C did not show the usual concentration dependence, implying that it does not dissociate easily into smaller species. The octamer could be dissociated by the use of reducing agents. The action of reduced glutathione on oligomeric betaG83C exhibited biphasic kinetics for the loss of the octameric form, with a time constant for the rapid phase of about 2 h at 1 mM glutathione. However, the size of oligomer betaG83C was not modified after incubation with fresh plasma.
authors.affiliation: INSERM U 473, 94276 Le Kremlin-Bicêtre Cedex, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Fablet
authors.forename: Michael C
authors.initials: MC
authors.lastname: Marden
authors.forename: Brian N
authors.initials: BN
authors.lastname: Green
authors.forename: Chien
authors.initials: C
authors.lastname: Ho
authors.forename: Josée
authors.initials: J
authors.lastname: Pagnier
authors.forename: Véronique
authors.initials: V
authors.lastname: Baudin-Creuza
mesh_terms: D019943:Amino Acid Substitution; D002850:Chromatography, Gel; D005978:Glutathione / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D013058:Mass Spectrometry; D013997:Time Factors
chemical_list: D006454:Hemoglobins; D005978:Glutathione