Detection of the human papillomavirus and analysis of the TP53 polymorphism of exon 4 at codon 72 in penile squamous cell carcinomas.
Human papillomaviruses (HPV) are thought to be involved in penile squamous cell carcinomas (SCC). A common polymorphism at codon 72 of exon 4 encoding either arginine (Arg) or proline (Pro) has been shown to affect HPV-mediated degradation of p53 in vitro, and may represent a risk factor for HPV-induced carcinogenesis. The presence of HPV DNA as well as the TP53 polymorphism at codon 72 of exon 4 were investigated in a series of 45 penile SCC. HPV detection and typing were carried out by polymerase chain reaction (PCR) with generic primers (MY09-MY11 and FAP59-FAP64), and type-specific DNA probes. TP53 polymorphism was further investigated using Denaturing Gradient Gel Electrophoresis (DGGE). HPV DNA was detected in 67% of penile SCC and 32% of benign lesions (BL) (P<0.05). Among the TP53 amplified samples, the rate of Arg homozygosity in penile SCC was 61% compared with 68% in BL (non-significant (NS)). Our results demonstrate a strong association between penile SCC and the presence of HPV DNA. The TP53 Arg/Arg genotype does not appear to represent a risk factor for the development of genital SCC in men, and no correlation was found between the TP53 polymorphism at codon 72 and the presence of HPV DNA.
Humbey
O
O
Department of Cell Biology-EA 3181-Institut d'Etude et de Transfert de Gènes, University Hospital, 25030 Besançon Cedex, France.
Cairey-Remonnay
S
S
Guérrini
J S
JS
Algros
M P
MP
Mougin
C
C
Bittard
H
H
Aubin
F
F
D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005091:Exons; D016158:Genes, p53; D005838:Genotype; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D027383:Papillomaviridae / Q000235:genetics; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000235:genetics; D010412:Penile Neoplasms / Q000235:genetics* / Q000821:virology; D011110:Polymorphism, Genetic; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000235:genetics
D004279:DNA, Viral