Novel "three-in-one" peptide device for genetic drug delivery.
We here describe a new strategy for the delivery of oligonucleotides to cells that is based on the use of a short peptide containing three functional units: a membrane-penetrating segment, a DNA-binding domain and a cell-localization sequence. The designed vector binds strongly to oligonucleotides and has membrane-perturbing abilities in vitro. This type of multi-functional device may be a powerful tool to achieve efficient delivery of genetic drugs in vivo.
Smith
Jason
J
Molecular and Cellular Biology Graduate Program, and Department of Chemistry, Tulane University, New Orleans, Louisiana 70118, USA.
Guidry
Jesse
J
Wittung-Stafshede
Pernilla
P
D000595:Amino Acid Sequence; D001854:Bone Marrow Cells / Q000166:cytology; D002462:Cell Membrane / Q000378:metabolism; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D009841:Oligonucleotides / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D013050:Spectrometry, Fluorescence; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism
D009841:Oligonucleotides; D009842:Oligopeptides; D004247:DNA