title: Differences between BALB/c and C57BL/6 mice in mouse hepatitis virus replication in primary hepatocyte culture.
abstract: We previously showed that an intraperitoneal infection with mouse hepatitis virus (MHV) resulted in acute hepatic failure accompanying extremely elevated viral growth in the liver in interferon-gamma-deficient BALB/c (BALB-GKO), but not C57BL/6 (B6-GKO) mice. To examine the basis of the strain difference against MHV infection in interferon-gamma-deficient mice, viral replication in primary hepatocyte cultures from BALB/c and B6 mice with or without the IFN-gamma gene was compared in vitro. The MHV replication in BALB/c hepatocytes with or without the IFN-gamma gene was significantly higher than that in B6 hepatocytes with or without the IFN-gamma gene, suggesting that there is a strain difference in MHV replication in hepatocytes. Since a significant difference in MHV replication in hepatocytes was not observed between wild type and IFN-gamma-deficient mice of the same genetic background, the phenomenon is thought to be independent of IFN-gamma. However, pretreatment of hepatocytes with recombinant mouse interferon-gamma inhibited MHV replication in a dose-dependent fashion. The results are discussed with respect to the pathology of MHV infection in mice with or without the IFN-gamma gene.
authors.affiliation: Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Tokyo 108-8639, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kyuwa
authors.forename: Seiji
authors.initials: S
authors.lastname: Kawamura
authors.forename: Yoh-ichi
authors.initials: Y
authors.lastname: Tagawa
authors.forename: Yoichiroh
authors.initials: Y
authors.lastname: Iwakura
authors.forename: Toru
authors.initials: T
authors.lastname: Urano
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018352:Coronavirus Infections / Q000235:genetics / Q000517:prevention & control / Q000821:virology; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000821:virology*; D007371:Interferon-gamma / Q000008:administration & dosage / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics* / Q000821:virology*; D008810:Mice, Inbred C57BL / Q000235:genetics* / Q000821:virology*; D006517:Murine hepatitis virus / Q000472:pathogenicity / Q000502:physiology*; D011994:Recombinant Proteins / Q000008:administration & dosage; D014779:Virus Replication
chemical_list: D011994:Recombinant Proteins; D007371:Interferon-gamma