title: Effect of tenascin and fibronectin on the migration of human corneal fibroblasts.
abstract: OBJECTIVE
  To investigate the effect of fibronectin and tenascin on the migration of corneal fibroblasts.
  
  
  METHODS
  Department of Ophthalmology, University of Vienna, Medical School, Vienna, Austria.
  
  
  METHODS
  Using human corneal fibroblasts, a monolayer migration assay was performed to measure corneal fibroblast movement. The migration on collagen I, fibronectin, and tenascin with and without transforming growth factor (TGF)-alpha/fibroblast growth factor (FGF)-2 stimulation and the effect of soluble tenascin and fibronectin on corneal fibroblast migration on collagen-I-coated wells were investigated.
  
  
  RESULTS
  The cytokines TGF-alpha and FGF-2 stimulated migrational activity of corneal stromal cells in a dose-dependent manner, reaching the maximum effect at 100 ng/mL and 10 ng/mL, respectively. The migration of corneal fibroblasts on fibronectin was significantly higher (P <.05) than the migration on collagen I. Transforming growth factor-alpha and FGF-2 increased radial cell displacement independent of the provided matrix composition. Tenascin had a negative effect on corneal fibroblast adhesion/migration in this in vitro model.
  
  
  CONCLUSIONS
  Fibronectin and tenascin influenced corneal fibroblast migration and adhesion, respectively, and may play a role in stromal cell movement during wound healing. The cytokines TGF-alpha and FGF-2 had an additive effect on corneal fibroblast migration on a fibronectin matrix.
authors.affiliation: Department of Ophthalmology, University of Vienna, Medical School, Vienna, Austria. schmidinger@gmx.at
authors.forename: Gerald
authors.initials: G
authors.lastname: Schmidinger
authors.forename: Georg
authors.initials: G
authors.lastname: Hanselmayer
authors.forename: Stefan
authors.initials: S
authors.lastname: Pieh
authors.forename: Birgit
authors.initials: B
authors.lastname: Lackner
authors.forename: Stephan
authors.initials: S
authors.lastname: Kaminski
authors.forename: Irene
authors.initials: I
authors.lastname: Ruhswurm
authors.forename: Christian
authors.initials: C
authors.lastname: Skorpik
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000502:physiology; D003315:Cornea / Q000166:cytology*; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D005353:Fibronectins / Q000502:physiology*; D006801:Humans; D019063:Tenascin / Q000502:physiology*; D016211:Transforming Growth Factor alpha / Q000494:pharmacology; D014746:Vimentin / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D005353:Fibronectins; D019063:Tenascin; D016211:Transforming Growth Factor alpha; D014746:Vimentin; D016222:Fibroblast Growth Factor 2