Identification of env CRF-10 among HIV variants circulating in rural western Kenya.
As part of a program to determine the genetic diversity of human immunodeficiency virus in rural Kenya, we carried out a molecular analysis of the C2-V3 region of HIV-infected blood samples obtained from 30 antenatal clinic attendees of seven health centers in western Kenya. Direct sequencing was carried out on the envelope C2-V3 region of proviral DNA. On phylogenetic analysis with reference strains, 20 were subtype Al, 2 were subtype D, 1 was subtype C, 1 was subtype G, 1 was CRF-10, 2A/D, 2A/C, and 2 were unclassified. The presence of CRF-10 and the great variety of subtypes and recombinants in such a limited sample size suggest that western Kenya may be a potential hotspot for HIV recombination in the country.
Songok
Elijah M
EM
Department of Viral Infection and International Health, Graduate School of Medical Sciences, Kanazawa University, Japan. Esongok@nairobi.mimcom.net
Lihana
Raphael W
RW
Kiptoo
Michael K
MK
Genga
Isaiah O
IO
Kibaya
Rukia
R
Odhiambo
Frida
F
Kobayashi
Kana
K
Ago
Yumi
Y
Ndembi
Nicaise
N
Okoth
Frederick
F
Fujiyama
Yoshihide
Y
Imanishi
Jiro
J
Ichimura
Hiroshi
H
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D004279:DNA, Viral / Q000097:blood; D015686:Gene Products, env / Q000235:genetics*; D014644:Genetic Variation; D015699:HIV Envelope Protein gp120 / Q000235:genetics; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology; D015497:HIV-1 / Q000145:classification* / Q000235:genetics; D006801:Humans; D007630:Kenya / Q000453:epidemiology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D010802:Phylogeny; D011533:Proviruses; D011995:Recombination, Genetic; D012424:Rural Population; D017422:Sequence Analysis, DNA
D004279:DNA, Viral; D015686:Gene Products, env; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments