title: Characterisation of humoral immune responses in dogs vaccinated with irradiated Ancylostoma caninum.
abstract: Infection with Ancylostoma caninum, an intestinal hookworm of dogs, can cause debilitating and potentially life-threatening disease. In the current study, protective immunity to hookworm infection was induced in dogs following vaccination with irradiation-attenuated third-stage larvae (L3) with significant reductions in both worm (P<0.03) and faecal egg counts (P<0.0004) following a challenge infection. Vaccination with irradiated L3 and challenge with infective L3 stimulated a dominant antibody response to antigens of less than 20 kDa in an excretory/secretory extract from adult parasites. Immunoscreening of an adult A. caninum cDNA library with antisera from the vaccine trial identified a number of clones. The three clones with the strongest immunoreactivity proved to be identical and encoded a peptide with similarity to the N-terminal domain of the tissue matrix metalloproteinase inhibitor (TIMP)-2 mammalian tissue metalloproteinase inhibitor family.
authors.affiliation: Victorian Institute of Animal Science, 475 Mickleham Road, 3049, Vic., Attwood, Australia
authors.forename: P R
authors.initials: PR
authors.lastname: Boag
authors.forename: J C
authors.initials: JC
authors.lastname: Parsons
authors.forename: P J A
authors.initials: PJ
authors.lastname: Presidente
authors.forename: T W
authors.initials: TW
authors.lastname: Spithill
authors.forename: J L
authors.initials: JL
authors.lastname: Sexton
mesh_terms: D000595:Amino Acid Sequence; D000722:Ancylostoma / Q000235:genetics / Q000276:immunology* / Q000528:radiation effects; D000724:Ancylostomiasis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western / Q000662:veterinary; D004283:Dog Diseases / Q000276:immunology / Q000469:parasitology*; D004285:Dogs; D005243:Feces / Q000469:parasitology; D005260:Female; D007422:Intestines / Q000469:parasitology; D008297:Male; D008969:Molecular Sequence Data; D010270:Parasite Egg Count / Q000662:veterinary; D016415:Sequence Alignment; D014611:Vaccination / Q000379:methods*; D014613:Vaccines, Attenuated / Q000276:immunology* / Q000592:standards
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D014613:Vaccines, Attenuated