Type I collagen regulated dentin matrix protein-1 (Dmp-1) and osteocalcin (OCN) gene expression of rat dental pulp cells.
In this study, we investigated the effect of type I collagen on dentin matrix protein-1 (Dmp-1) and osteocalcin (OCN) gene expression of dental pulp cells. The mRNA level of Dmp-1 gene was down-regulated; however, OCN gene expression was up-regulated by the culture of dental pulp cells with type I collagen. These findings imply that type I collagen regulates mRNA level of Dmp-1 and OCN gene that are predominantly expressed in active odontoblasts. The change of gene expression by type I collagen was suppressed by the blocking of collagen-integrin interaction. We could conclude that the effect of type I collagen was mediated via binding of collagen to integrin receptors.
Mizuno
Morimichi
M
Department of Oral Health Science, School of Dentistry, Hokkaido University, Sapporo, Japan 060. mmizuno@den.hokudai.ac.jp
Miyamoto
Tetsuro
T
Wada
Keinoshin
K
Watatani
Sanae
S
Zhang
Gui Xia
GX
D000469:Alkaline Phosphatase / Q000032:analysis / Q000096:biosynthesis; D000818:Animals; D002478:Cells, Cultured; D024042:Collagen Type I / Q000494:pharmacology / Q000502:physiology*; D003782:Dental Pulp / Q000187:drug effects / Q000378:metabolism*; D003804:Dentin / Q000378:metabolism*; D016326:Extracellular Matrix Proteins; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009804:Odontoblasts / Q000378:metabolism; D015675:Osteocalcin / Q000032:analysis / Q000096:biosynthesis*; D010750:Phosphoproteins / Q000032:analysis / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation
D024042:Collagen Type I; C081635:Dmp1 protein, rat; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D015675:Osteocalcin; D000469:Alkaline Phosphatase