Cloning and expression of a novel, truncated, progesterone receptor.
Progesterone acts via two specific receptors to affect gene transcription in target tissues. Progesterone receptor (PR) B contains 933 amino acids while PR A is a truncated version lacking the initial 164 amino acids. We have cloned a novel, truncated PR from both human adipose and aortic cDNA libraries. This cDNA encodes a predicted protein of 314 amino acids, termed PR-M. Initiation of transcription of PR-M occurs in intron 3, with the initial exon identical to exon 4 of the genomic PRs, except for a novel 16 amino acid amino-terminal sequence, consistent with a signal peptide. The remainder of PR-M is identical to the genomic PR. Transcript for this protein was identified by RT-PCR in human aortic endothelial cells and T47D breast cancer cells. Expression of PR-M in Sf 9 insect cells results in a 38-kDa protein, demonstrated in human aortic endothelial cells and T47D breast cancer cells. The function of PR-M remains to be determined. The presence of a signal peptide and the lack of a DNA binding region suggests a non-genomic action.
Saner
Karla J
KJ
Department of Microbiology and Molecular Medicine, Clemson University, SC, USA.
Welter
Brenda H
BH
Zhang
Fan
F
Hansen
Elizabeth
E
Dupont
Barbara
B
Wei
Yangzhan
Y
Price
Thomas M
TM
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D042783:Endothelial Cells / Q000378:metabolism; D005260:Female; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals; D017434:Protein Structure, Tertiary; D011980:Receptors, Progesterone / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D021382:Protein Sorting Signals; D011980:Receptors, Progesterone