Lipid peroxidation and vitamin E in human coronary atherosclerotic lesions.
BACKGROUND
We aimed to assess the oxidant/antioxidant status within the ex vivo human coronary endarterectomy samples.


METHODS
To achieve this, we measured products of lipid oxidation (malondialdehyde, 7-ketocholesterol), lipids (cholesterol, cholesteryl esters) and vitamin E in endarterectomy samples.


RESULTS
Content of malondialdehyde in the plaque ranged from 0.23 to 37.36 microg/g. Unesterified cholesterol content ranged from 0.30 to 1.94 mg/g. It was 9.04+/-4.32% of total cholesterol. Total cholesterol content ranged from 1.73 to 23.69 mg/g. Cholesteryl palmitate content ranged from 0.57 to 19.10 mg/g, which is 11.43-60.86% of the total esters (mean+/-SD 40.27+/-18.42%). Cholesteryl oleate content ranged from 0.24 to 5.76 mg/g, being 9.97-21.81% of total esters (mean+/-SD 14.35+/-4.51%). Cholesteryl linoleate content ranged from 1.05 to 8.21 mg/g, being 17.84-45.15% of total esters (mean+/-SD 30.78+/-11.69%). Cholesteryl arachidonate content ranged from 0.51 to 4.20 mg/g, which is 7.56-22.87% of total esters (mean+/-SD 14.60+/-5.60%). The cholesteryl linoleate/cholesteryl oleate ratio (CL/CO) ranged from 1.01 to 4.33. Content of 7-ketocholesterol in the plaque ranged from 0.0 to 577.5 ng/g of wet weight. The 7-ketocholesterol/total cholesterol ratio was 0.003+/-0.003% (range from 0.0% to 0.008%). The 7-ketocholesterol/unesterified cholesterol ratio was 0.024+/-0.023% (range from 0.0% to 0.066%). The plaque content of vitamin E ranged from 0.0 to 40.9 microg/g of wet weight.


CONCLUSIONS
The present study, comprising measurements of lipids, products of lipid peroxidation and vitamin E in 12 human coronary endarterectomy samples, lends the evidence for ongoing lipid peroxidation within an atherosclerotic lesion.
Jacheć
Wojciech
W
II Department of Cardiology, Silesian School of Medicine, Ul. Skłodowskiej 10, Zabrze 41-800, Poland.
Tomasik
Andrzej
A
Ceglarek
Wojciech
W
Woś
Stanisław
S
Wodniecki
Jan
J
Wojciechowska
Celina
C
Skrzep-Poloczek
Bronisława
B
Walichiewicz
Piotr
P
Widenka
Kazimierz
K
D000975:Antioxidants / Q000378:metabolism; D002784:Cholesterol / Q000031:analogs & derivatives / Q000032:analysis; D003324:Coronary Artery Disease / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D004691:Endarterectomy; D006801:Humans; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000032:analysis; D016877:Oxidants / Q000378:metabolism; D013223:Statistics as Topic; D014810:Vitamin E / Q000032:analysis* / Q000378:metabolism
D000975:Antioxidants; D016877:Oxidants; D014810:Vitamin E; D008315:Malondialdehyde; D002784:Cholesterol