Inhibitory effects of luteolin isolated from Ixeris sonchifolia Hance on the proliferation of HepG2 human hepatocellular carcinoma cells.
We investigated the anti-proliferative effects of luteolin and apigenin, isolated from Ixeris sonchifolia Hance, on HepG2 human hepatocellular carcinoma cells. In MTT assay luteolin showed more efficient anti-proliferative effects on cells than apigenin did. According to propidium iodide staining and flow cytometry studies, we postulated that these effects might be a result of cell cyde arrest. Hence we examined the changes of protein expressions related to cell cycle arrest. Western blotting data demonstrated that the down-regulated expression of CDK4 was correlated to the increase of p53 and CDK inhibitor p21(WAF1/CIP1) protein. These data suggest that luteolin may have potential as an anti-cancer agent.
Yee
Su Bog
SB
Department of Pharmacy, Pusan National University, Busan 609-735, Korea.
Lee
Jung Hwa
JH
Chung
Hae Young
HY
Im
Kwang Sik
KS
Bae
Song Ja
SJ
Choi
Jae Soo
JS
Kim
Nam Deuk
ND
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D047310:Apigenin; D019659:Asteraceae / Q000737:chemistry*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D051358:Cyclin-Dependent Kinase 4; D050759:Cyclin-Dependent Kinase Inhibitor p21; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis; D016213:Cyclins / Q000096:biosynthesis; D015536:Down-Regulation; D004354:Drug Screening Assays, Antitumor; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D047311:Luteolin; D018517:Plant Roots / Q000737:chemistry; D011518:Proto-Oncogene Proteins; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D015854:Up-Regulation
D000972:Antineoplastic Agents, Phytogenic; C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D005419:Flavonoids; D011518:Proto-Oncogene Proteins; D016159:Tumor Suppressor Protein p53; D047310:Apigenin; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases; D047311:Luteolin