title: Isolation of Escherichia coli O157:H7 from intact colon fecal samples of swine.
abstract: Escherichia coli O157:H7 was recovered from colon fecal samples of pigs. Polymerase chain reaction confirmed two genotypes: isolates harboring the eaeA, stx(1), and stx(2) genes and isolates harboring the eaeA, stx(1), and hly(933) genes. We demonstrate that swine in the United States can harbor potentially pathogenic E. coli O157:H7.
authors.affiliation: Microbial Biophysics and Residue Chemistry and Core Technologies, U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania 19038, USA. ifeder@arserrc.gov
authors.forename: Ingrid
authors.initials: I
authors.lastname: Feder
authors.forename: F Morgan
authors.initials: FM
authors.lastname: Wallace
authors.forename: Jeffrey T
authors.initials: JT
authors.lastname: Gray
authors.forename: Pina
authors.initials: P
authors.lastname: Fratamico
authors.forename: Paula J
authors.initials: PJ
authors.lastname: Fedorka-Cray
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Pearce
authors.forename: Jeffrey E
authors.initials: JE
authors.lastname: Call
authors.forename: Richard
authors.initials: R
authors.lastname: Perrine
authors.forename: John B
authors.initials: JB
authors.lastname: Luchansky
mesh_terms: D000818:Animals; D016521:Electrophoresis, Gel, Pulsed-Field; D019453:Escherichia coli O157 / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D013552:Swine / Q000382:microbiology*