Reduced expression of calsenilin/DREAM/KChIP3 in the brains of kainic acid-induced seizure and epilepsy patients.
Calsenilin is a neuronal calcium binding protein that may function in calcium signaling and cell death. Kainic acid, an analog of the excitatory amino acid L-glutamate, produced excitotoxic cell death and induced the pathophysiology of status epilepticus. The expression of calsenilin was investigated in the mouse brain after kainic acid-induced seizure and seizure-induced hippocampal neuronal cell culture system using immunostaining analysis. Calsenilin was markedly decreased not only in the damaged cortex and CA3 region of hippocampus at 24 h after kainic acid-induced seizure but also in a cell-culture model of seizure-like activity. In addition, immunoreactivity of calsenilin in the hippocampus derived from human epilepsy patient was significantly decreased compared with normal brain. These results demonstrate that the reduced expression of calsenilin may functionally be associated with the pathophysiology of status epilepticus.
Hong
Yeon-Mi
YM
Department of Life Science, Kwangju Institute of Science and Technology, 1 Oryong-dong, Puk-gu, Kwangju 500-712, South Korea.
Jo
Dong-Gyu
DG
Lee
Min-Cheol
MC
Kim
So-Young
SY
Jung
Yong-Keun
YK
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D015536:Down-Regulation; D004827:Epilepsy / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007608:Kainic Acid / Q000633:toxicity; D051605:Kv Channel-Interacting Proteins; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010361:Patients / Q000706:statistics & numerical data; D012097:Repressor Proteins / Q000096:biosynthesis*; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*
D002135:Calcium-Binding Proteins; C496756:Csen protein, mouse; C496749:KCNIP3 protein, human; D051605:Kv Channel-Interacting Proteins; D012097:Repressor Proteins; D007608:Kainic Acid