Gelsolin is a dorsalizing factor in zebrafish.
The gene for gelsolin (an actin-binding, cytoskeletal regulatory protein) was shown earlier to be specialized for high corneal expression in adult zebrafish. We show here that zebrafish gelsolin is required for proper dorsalization during embryogenesis. Inhibition of gelsolin expression by injecting fertilized eggs with a specific morpholino oligonucleotide resulted in a range of concentration-dependent ventralized phenotypes, including those lacking a brain and eyes. These were rescued by coinjection of zebrafish gelsolin or chordin (a known dorsalizing agent) mRNAs, or human gelsolin protein. Moreover, injection of gelsolin mRNA or human gelsolin protein by itself dorsalized the developing embryos, often resulting in axis duplication. Injection of the gelsolin-specific morpholino oligonucleotide enhanced the expression of Vent mRNA, a ventral marker downstream of bone morphogenetic proteins, whereas injection of gelsolin mRNA enhanced the expression of chordin and goosecoid mRNAs, both dorsal markers. Our results indicate that gelsolin also modulates embryonic dorsalventral pattern formation in zebrafish.
Kanungo
Jyotshnabala
J
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, 6 Center Drive, Bethesda, MD 20892, USA.
Kozmik
Zbynek
Z
Swamynathan
Shivalingappa K
SK
Piatigorsky
Joram
J
D000818:Animals; D001483:Base Sequence; D019521:Body Patterning / Q000235:genetics / Q000502:physiology; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D003315:Cornea / Q000196:embryology / Q000378:metabolism; D018260:Gelsolin / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D006023:Glycoproteins / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics; D015398:Signal Transduction; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000502:physiology*; D029961:Zebrafish Proteins / Q000235:genetics
D019485:Bone Morphogenetic Proteins; D018260:Gelsolin; D006023:Glycoproteins; D018398:Homeodomain Proteins; D036341:Intercellular Signaling Peptides and Proteins; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D012097:Repressor Proteins; D029961:Zebrafish Proteins; C434250:vent protein, zebrafish; C054234:chordin