Fluorescence in situ hybridization (FISH) on human chromosomes using photoprobe biotin-labeled probes.
Fluorescence in situ hybridization (FISH) on human chromosomes in meta- and interphase is a well-established technique in clinical and tumor cytogenetics and for studies of evolution and interphase architecture. Many different protocols for labeling the DNA probes used for FISH have been published. Here we describe for the first time the successful use of Photoprobe biotin-labeled DNA probes in FISH experiments. Yeast artificial chromosome (YAC) and whole chromosome painting (wcp) probes were tested.
Weise
Anja
A
Institute of Human Genetics, Jena, Germany.
Harbarth
Peter
P
Claussen
Uwe
U
Liehr
Thomas
T
D001710:Biotin / Q000737:chemistry; D020223:Chromosome Painting / Q000379:methods; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D015342:DNA Probes / Q000737:chemistry; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*
D015342:DNA Probes; D001710:Biotin