Synergic and competitive inhibition effect between two kinds of arylamines in the formation of hemoglobin adduct in vitro.
This article presents our work with HPLC-UV to determine hemoglobin adducts produced by adduction reaction among 4-aminobiphenyl (4-ABP) or/and 2-naphthylamine (2-NA) and human hemoglobin in vitro. The results showed that HPLC-UV could efficiently be used to detect aromatic amine-hemoglobin adduct. Two kinds of optimized linear models modified from the traditional dose-response model were screened out. The models implied that synergic effect exists between the two kinds of arylamines adducted with hemoglobin and competitive inhibition effect occurs in the formation of hemoglobin adduct when the two kinds of arylamines are present in the blood synchronously. The results indicate that models for dose-response relationship should take the effects of the other toxicants into consideration in the mixed pollutant system.
Chen
Hui-Ming
HM
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
Qin
Tao
T
Xu
Xiao-Bai
XB
D015081:2-Naphthylamine / Q000737:chemistry*; D000611:Aminobiphenyl Compounds / Q000737:chemistry*; D002273:Carcinogens / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006454:Hemoglobins / Q000737:chemistry*; D066298:In Vitro Techniques; D008962:Models, Theoretical
D000611:Aminobiphenyl Compounds; D002273:Carcinogens; D006454:Hemoglobins; C006757:4-biphenylamine; D015081:2-Naphthylamine