title: Alterations of cullin-5 mRNA levels in the rat central nervous system following hemorrhagic shock.
abstract: Hemorrhagic shock is a clinical syndrome that manifests as hypoperfusion, hypoxia, and ischemia initiating various cellular stress responses involved in the synthesis and release of an assortment of pro-inflammatory molecules, cytokines, chemokines, and reactive oxidant species (ROS). The ROS have been shown to oxidize and damage proteins making them targets for ubiquitination and proteasomal degradation. Cullin-5 (cul-5), an E3 ligase that binds ubiquitin to proteins targeted for degradation via the proteasome, was investigated for its gene expression during hemorrhagic shock. Male Long-Evans rats were subjected to volume controlled (27 ml kg-1) hemorrhage over 10 min and kept in shock for 60 min. Quantitative realtime polymerase chain reaction showed cul-5 mRNA levels were significantly increased in the brainstem and cerebellum, and decreased in the hypothalamus of rats as a result of hemorrhagic shock (n = 6) compared to sham-treated rats (n = 6). Cul-5 mRNA levels in the cerebral cortex, small intestine, kidney, liver, lung, or pituitary gland did not significantly change after hemorrhagic shock. This is the first report of cul-5 mRNA regulation by hemorrhagic shock. Evidence indicates this protein may have a regulatory role in ubiquitin-proteasomal protein degradation in response to hemorrhagic shock.
authors.affiliation: Graduate Program in Neuroscience, Department of Surgery, F. Edmund Hébert School of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA. Tceremuga@USUHS.mil
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Ceremuga
authors.forename: Xiang-Lan
authors.initials: XL
authors.lastname: Yao
authors.forename: Hasan B
authors.initials: HB
authors.lastname: Alam
authors.forename: Joseph T
authors.initials: JT
authors.lastname: McCabe
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology*; D044842:Cullin Proteins; D003546:Cysteine Endopeptidases / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D020318:Rats, Long-Evans; D017483:Receptors, Vasopressin / Q000235:genetics* / Q000378:metabolism*; D012771:Shock, Hemorrhagic / Q000378:metabolism / Q000503:physiopathology*; D025801:Ubiquitin / Q000378:metabolism
chemical_list: C094547:Cul5 protein, rat; D044842:Cullin Proteins; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D017483:Receptors, Vasopressin; D025801:Ubiquitin; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex