title: Enhancement of humoral immune responses to a human cytomegalovirus DNA vaccine: adjuvant effects of aluminum phosphate and CpG oligodeoxynucleotides.
abstract: A human cytomegalovirus (HCMV) glycoprotein B (gpUL55) DNA vaccine has been evaluated in BALB/c mice. Intramuscular immunization of these mice with pRc/CMV2-gB resulted in the generation of high levels of gpUL55-specific antibody (geometric mean titer [GMT] 1:8900) and neutralizing antibody (GMT 1:74) after 2 booster doses given 5 and 10 weeks after primary inoculation. Emulsifying the construct with the aluminum phosphate gel adjuvant Adju-Phos before immunization enhanced gpUL55-specific antibody responses (GMT 1:17800, P = 0.04). Co-immunization with CpG oligodeoxynucleotides was shown to enhance levels of neutralizing antibodies generated by immunization of mice with a pRc/CMV2-gB/Adju-Phos emulsion (P = 0.04). The results provide a rationale for evaluating combinations of other HCMV proteins for incorporation into a multi-target DNA vaccine, and for the optimization of adjuvant usage, to elicit enhanced levels of neutralizing antibodies. 2003.
authors.affiliation: Department of Virology, Royal Free and University College Medical School, London, United Kingdom.
authors.forename: Nigel J
authors.initials: NJ
authors.lastname: Temperton
authors.forename: Debra C
authors.initials: DC
authors.lastname: Quenelle
authors.forename: Keirissa M
authors.initials: KM
authors.lastname: Lawson
authors.forename: Jane N
authors.initials: JN
authors.lastname: Zuckerman
authors.forename: Earl R
authors.initials: ER
authors.lastname: Kern
authors.forename: Paul D
authors.initials: PD
authors.lastname: Griffiths
authors.forename: Vincent C
authors.initials: VC
authors.lastname: Emery
mesh_terms: D000276:Adjuvants, Immunologic; D017607:Aluminum Compounds / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007117:Immunization, Secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage / Q000276:immunology*; D010710:Phosphates / Q000276:immunology*; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D017607:Aluminum Compounds; C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides; D010710:Phosphates; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C012714:aluminum phosphate