Construction and integration of radiation-hybrid and cytogenetic maps of dog Chromosome X.
Chromosome (chr) X is under-represented in current maps of the genome of the domestic dog ( Canis familiaris). To address this problem, we have constructed a small-insert, genomic DNA library in pBluescript from flow-sorted canine Chr X DNA. Fluorescence in situ hybridization (FISH) studies confirmed that the library was highly enriched for Chr X. Clones containing microsatellites were identified and sequenced. Database searches detected significant sequence identity between four X-derived clones and genes previously characterized in other species. Thirty-seven markers derived from these clones were mapped on Chr X by FISH, and of these, 28 were mapped by using the female-derived T72 whole-genome radiation hybrid (RH) panel (Research Genetics). Four X-linked canine genes from publicly available data were also mapped. Eight RH linkage groups with LOD >4.0 were identified, and FISH data were used to locate the groups on the chromosome; four groups could be unambiguously orientated by FISH data. In each case, the FISH and RH data were mutually consistent. The data suggest strongly conserved synteny between canine and human X Chrs. The pseudoautosomal region has been further characterized, and the putative or actual locations of nine genes of clinical relevance have been suggested.
Spriggs
Helen F
HF
Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk, CB8 7UU, UK. hfs@mrc-lmb.cam.ac.uk
Holmes
Nigel G
NG
Breen
Matthew G
MG
Deloukas
Panos G
PG
Langford
Cordelia F
CF
Ross
Mark T
MT
Carter
Nigel P
NP
Davis
Maria E
ME
Knights
Catherine E
CE
Smith
Angela E
AE
Farr
Christine J
CJ
McCarthy
Linda C
LC
Binns
Matthew M
MM
D000818:Animals; D017931:DNA Primers / Q000378:metabolism; D018900:Dinucleotide Repeats; D004285:Dogs; D017404:In Situ Hybridization, Fluorescence; D008126:Lod Score; D008969:Molecular Sequence Data; D021101:Radiation Hybrid Mapping; D017422:Sequence Analysis, DNA; D014960:X Chromosome
D017931:DNA Primers