title: Gas chromatography-olfactometry (GC-O) and proton transfer reaction-mass spectrometry (PTR-MS) analysis of the flavor profile of grana padano, parmigiano reggiano, and grana trentino cheeses.
abstract: Gas chromatography-olfactometry (GC-O) and proton transfer reaction-mass spectrometry (PTR-MS) techniques were used to deduce the profile of odor-active and volatile compounds of three grana cheeses: Grana Padano (GP), Parmigiano Reggiano (PR), and Grana Trentino (GT). Samples for GC-O analysis were prepared by dynamic headspace extraction, while a direct analysis of the headspace formed over cheese was performed by PTR-MS. The major contributors to the odor profile were ethyl butanoate, 2-heptanone, and ethyl hexanoate, with fruity notes. A high concentration of mass 45, tentatively identified as acetaldehyde, was found by PTR-MS analysis. Low odor threshold compounds, e.g., methional and 1-octen-3-one, which contributed to the odor profile but were not detected by FID, were detected by PTR-MS. Principal component analysis on both GC-O and PTR-MS data separated the three cheese samples well and showed specific compounds related to each sample.
authors.affiliation: Institut für Ionenphysik, Universität Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria.
authors.forename: Elena
authors.initials: E
authors.lastname: Boscaini
authors.forename: Saskia
authors.initials: S
authors.lastname: van Ruth
authors.forename: Franco
authors.initials: F
authors.lastname: Biasioli
authors.forename: Flavia
authors.initials: F
authors.lastname: Gasperi
authors.forename: Tilmann D
authors.initials: TD
authors.lastname: Märk
mesh_terms: D000328:Adult; D002611:Cheese / Q000032:analysis*; D002849:Chromatography, Gas; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D009812:Odorants / Q000032:analysis*; D011522:Protons; D012903:Smell; D014835:Volatilization
chemical_list: D011522:Protons