Cellular factors and IE62 activation of VZV promoters.
The varicella zoster virus IE62 protein transactivates promoters representing all three kinetic classes of viral genes. This is accomplished in conjunction with the general transcription machinery of the cell and specific cellular transcription factors that act at sites located within viral promoters. The incidence of a subset of specific recognition sites was examined for the ubiquitous cellular factors Sp1 and USF within the VZV genome. Evidence is also presented for a direct physical interaction between Sp1 and IE62.
Ruyechan
William T
WT
Department of Microbiology, 138 Farber Hall, University at Buffalo, Buffalo, NY 14214, USA. ruyechan@buffalo.edu
Peng
Hua
H
Yang
Min
M
Hay
John
J
D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins; D005814:Genes, Viral; D014645:Herpesvirus 3, Human / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics* / Q000378:metabolism*; D066298:In Vitro Techniques; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D016329:Sp1 Transcription Factor / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D015533:Transcriptional Activation; D051720:Upstream Stimulatory Factors; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism*
D004279:DNA, Viral; D004268:DNA-Binding Proteins; C074221:IE62 protein, Human herpesvirus 3; D017874:Immediate-Early Proteins; D016329:Sp1 Transcription Factor; D015534:Trans-Activators; D014157:Transcription Factors; C497181:USF1 protein, human; D051720:Upstream Stimulatory Factors; D014759:Viral Envelope Proteins