title: Tigliane diterpenes from the latex of Euphorbia obtusifolia with inhibitory activity on the mammalian mitochondrial respiratory chain.
abstract: Six diterpenes isolated from the latex of Euphorbia obtusifolia Poir. (Euphorbiaceae) were evaluated for their inhibition of the NADH oxidase activity in submitochondrial particles from beef heart. 4,20-Dideoxyphorbol-12,13-bis(isobutyrate) was the most potent inhibitor and showed an inhibitory concentration with IC(50) value of 2.6+/-0.3mM. In the present study, some structure-activity trends are suggested for the inhibitory activity of the mammalian mitochondrial respiratory chain of these natural product derivatives of 4-deoxyphorbol esters.
authors.affiliation: Grupo de Reproducción, Universidad de Antioquia, A.A. 1226, Medellín, Colombia. labeta@catios.udea.edu.co
authors.forename: Liliana
authors.initials: L
authors.lastname: Betancur-Galvis
authors.forename: Emilio
authors.initials: E
authors.lastname: Palomares
authors.forename: J Alberto
authors.initials: JA
authors.lastname: Marco
authors.forename: Ernesto
authors.initials: E
authors.lastname: Estornell
mesh_terms: D000818:Animals; D002417:Cattle; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D004579:Electron Transport / Q000187:drug effects*; D028481:Euphorbia / Q000737:chemistry*; D066298:In Vitro Techniques; D007840:Latex / Q000737:chemistry*; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D012402:Rotenone / Q000494:pharmacology; D014475:Uncoupling Agents / Q000494:pharmacology
chemical_list: D004224:Diterpenes; D007840:Latex; D010936:Plant Extracts; D014475:Uncoupling Agents; D012402:Rotenone; D009247:NADH, NADPH Oxidoreductases