Marker assisted detection of gene (1Dx5) and translocation (1B/1R) in wheat genotypes.
Detection of 1Dx5 gene and presence of 1B/1R wheat rye translocation were studied in nineteen elite Indian wheat genotypes using AS-PCR and STS markers, respectively. Fifteen genotypes had 1B/1R translocation whereas ten showed presence of 1Dx5 gene. More than 50 per cent of the genotypes tested were found positive for both 1Dx5 and 1B/1R translocation. The results are in conformity with HMW glutenin SDS-PAGE profile for 1Dx5 and cytological observations for 1B/1R translocation.
Tiwari
Ratan
R
Directorate of Wheat Research, P.O. Box 158, Karnal 132 001, India. tiwari 1964@yahoo.com
Priyamvada
Singh
Rajender
R
Nainawatee
H S
HS
Kumar
Rajendra
R
Tyagi
B S
BS
Gupta
R K
RK
Nagarajan
S
S
D017343:Genes, Plant; D005819:Genetic Markers; D005838:Genotype; D005983:Glutens / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics; D007194:India; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*
D005819:Genetic Markers; D005983:Glutens; C037302:glutenin