The SEIRA spectroscopy data of nucleic acids and phospholipids from sensitive- and drug-resistant rat tumours.
It is known that tumour progression towards drug resistance is one of the main factors resulting in the failure of cancer treatment. As tumour progression is based on the genetic instability, the study of the structure of nucleic acid from tumour cells is of great importance both for basic knowledge and for biomedical application. We applied surface enhanced infrared absorption spectroscopy (SEIRA) of nucleic acids on gold substrate and essentially increased the sensitivity of IR spectroscopy. We observed numerous changes in infrared spectra of DNA from sensitive and resistant cells that reflect drastic changes in molecular structure of DNA from tumour cells. The DNA from resistant cancer cells could be characterised as rigid structure, the structure of DNA from sensitive cancer strain seems to be flexible and after application of anticancer drugs drastically changes and approaches to the structure of helix forms. The molecular structure of lipids from resistant and sensitive cancers after application of anti-tumour drugs is also modified. Thus, we observed a disordering in the lipid chain packing from resistant cells after application of cisplatin and, in some cases, formation of phospholipid-Pt complex.
Chekhun
V F
VF
Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, Kyiv, Ukraine. kul@onconet.kiev.ua
Solyanik
G I
GI
Kulik
G I
GI
Tryndiak
V P
VP
Todor
I N
IN
Dovbeshko
G I
GI
Repnytska
O P
OP
D000818:Animals; D004273:DNA, Neoplasm / Q000032:analysis*; D004317:Doxorubicin / Q000627:therapeutic use; D019008:Drug Resistance, Neoplasm; D007091:Image Processing, Computer-Assisted; D018625:Microscopy, Atomic Force; D009374:Neoplasms, Experimental / Q000737:chemistry* / Q000188:drug therapy; D010743:Phospholipids / Q000032:analysis*; D051381:Rats; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D004273:DNA, Neoplasm; D010743:Phospholipids; D004317:Doxorubicin