Protection against Leishmania major infection by oligomannose-coated liposomes.
Liposomes coated with neoglycolipids constructed with mannopentaose and dipalmitoylphosphatidylethanolamine (Man5-DPPE) have been shown to induce cellular immunity against antigens encapsulated in the liposomes. To assess whether these neoglycolipid-coated liposomes can elicit protective immune response against challenge infection, effects of immunization with soluble leishmanial antigens encapsulated in the liposomes were evaluated using Leishmania major infection in susceptible BALB/c mice. Intraperitoneal immunization of mice with leishmanial antigens in the Man5-DPPE-coated liposomes significantly suppressed footpad swelling in comparison to the control, non-immunized mice, while progression of the disease was observed in mice administered antigens in uncoated liposomes and those administered soluble antigens alone, as seen with control mice. Similarly, the number of parasites decreased substantially in local lymph nodes of mice immunized with the antigen in the Man5-DPPE-coated liposomes. Protection against L. major infection in the immunized mice also coincided with an elevated ratio of antigen-specific IgG2a/IgG1 antibodies, which is a profile of T helper-type 1-like immune response. Taken together, these results indicate the possibility that Man5-DPPE-coated liposome-encapsulated antigens could serve as a vaccine that triggers protection against infectious disease.
Shimizu
Yoshitaka
Y
The Institute of Glycotechnology and the Department of Applied Biochemistry, Tokai University, Kanagawa Hiratsuka, 259-1292, Japan.
Yamakami
Kazuo
K
Gomi
Takao
T
Nakata
Munehiro
M
Asanuma
Hideki
H
Tadakuma
Takushi
T
Kojima
Naoya
N
D000276:Adjuvants, Immunologic; D000818:Animals; D002784:Cholesterol; D005079:Excipients; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D018320:Leishmania major / Q000276:immunology*; D007896:Leishmaniasis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D008081:Liposomes; D008198:Lymph Nodes / Q000469:parasitology; D008358:Mannose / Q000031:analogs & derivatives* / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009844:Oligosaccharides / Q000494:pharmacology*; D010270:Parasite Egg Count; D010714:Phosphatidylethanolamines; D016052:Protozoan Vaccines / Q000008:administration & dosage* / Q000276:immunology*
D000276:Adjuvants, Immunologic; D005079:Excipients; D007074:Immunoglobulin G; D008081:Liposomes; D009844:Oligosaccharides; D010714:Phosphatidylethanolamines; D016052:Protozoan Vaccines; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; D002784:Cholesterol; D008358:Mannose