Altered enzymes in ageing human fibroblasts.
Diploid human fibroblasts accumulate heat labile enzymes during the final stages of their life-span in culture. The RNA base analogue 5-FU induces premature senescence, which is preceded by the appearance of altered enzyme. These observations support Orgel's "error catastrophe" theory of ageing.
Holliday
R
R
National Institute for Medical Research, Mill Hill, London NW7 1AA.
Tarrant
G M
GM
D016923:Cell Death; D002460:Cell Line; D016922:Cellular Senescence / Q000187:drug effects; D004795:Enzyme Stability; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology*; D005472:Fluorouracil / Q000494:pharmacology; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D008297:Male; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism*; D013379:Substrate Specificity
D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005472:Fluorouracil