title: Functional post-translational proteomics approach to study the role of N-glycans in the development of Caenorhabditis elegans.
abstract: Glycosylation is one of the most common post-translational protein modifications. Carbohydrate-mediated interactions between cells and their environment are important in differentiation, embryogenesis, inflammation, cancer and metastasis and other processes. Humans and mice with mutations that prevent normal N-glycosylation show multi-systemic defects in embryogenesis, thereby proving that these molecules are essential for normal development; however, a large number of proteins undergo defective glycosylation in these human and mouse mutants, and it is therefore difficult to determine the precise molecular roles of specific N-glycans on individual proteins. We describe here a 'functional post-translational proteomics' approach that is designed to determine the role of N-glycans on individual glycoproteins in the development of Caenorhabditis elegans.
authors.affiliation: Department of Structural Biology and Biochemistry, Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada. harry@sickkids.on.ca
authors.forename: Harry
authors.initials: H
authors.lastname: Schachter
authors.forename: Shihao
authors.initials: S
authors.lastname: Chen
authors.forename: Wenli
authors.initials: W
authors.lastname: Zhang
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Spence
authors.forename: Shaoxian
authors.initials: S
authors.lastname: Zhu
authors.forename: John W
authors.initials: JW
authors.lastname: Callahan
authors.forename: Don J
authors.initials: DJ
authors.lastname: Mahuran
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Fan
authors.forename: Rick D
authors.initials: RD
authors.lastname: Bagshaw
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: She
authors.forename: J Cesar
authors.initials: JC
authors.lastname: Rosa
authors.forename: Vernon N
authors.initials: VN
authors.lastname: Reinhold
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry / Q000254:growth & development*; D013058:Mass Spectrometry; D011134:Polysaccharides / Q000737:chemistry / Q000502:physiology*; D011499:Protein Processing, Post-Translational; D040901:Proteomics
chemical_list: D011134:Polysaccharides