title: [ANCA's- approach, advantages, limits].
abstract: OBJECTIVE
  The aim of this paper is to present the experience of the Laboratory of the Department of Histology in establishing the presence of ANCAs, in cases which cover an extremely large and diverse pathological area. The investigations were made following the request of some clinical medical services, ANCAs being a complementary exam, with an important role in the orientation and/or confirmation of the diagnosis.
  
  
  METHODS
  There were investigated 77 cases with clinical diagnoses ranging in the connective tissue diseases field, mainly SLE and vasculitides, and in the renal diseases field, mainly glomerulonephritis. There was performed an indirect immunofluorescence technique with a personal method, involving the usage of blood smear instead of neutrophils concentrate, human anti-IgG monospecific fluorescent conjugate and serum from the patients.
  
  
  CONCLUSIONS
  There was obtained a comprehensive survey of the presence of ANCAs in the study group. The interpretation of the results was completed within the general clinical background and in association with the physician. There is discussed the significance of the data in comparison with the literature.
  
  
  CONCLUSIONS
  The ANCAs research may become a monitoring method of different cases, offering therapeutic and prognostic efficiency elements.
authors.affiliation: Facultatea de Medicină Stomatologică, Universitatea de Medicină şi Farmacie Gr. T. Popa Iaşi.
authors.forename: Irina-Draga
authors.initials: ID
authors.lastname: Căruntu
authors.forename: C
authors.initials: C
authors.lastname: Cotuţiu
authors.forename: Gabriela Cristina
authors.initials: GC
authors.lastname: Cântec
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005921:Glomerulonephritis / Q000097:blood; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D014657:Vasculitis / Q000097:blood
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic