Mucosal immunization with a genetically engineered pertussis toxin S1 fragment-cholera toxin subunit B chimeric protein.
A chimeric protein consisting of a divalent pertussis toxin (PT) S1 fragment linked to the cholera toxin (Ctx) A(2)B fragment was constructed. The chimera induced a mucosal immunoglobulin A (IgA) and a serum IgG immune response to PT and CtxB in BALB/c mice following intranasal immunization. The immune sera neutralized PT in vitro. In the mouse model of Bordetella pertussis respiratory infection, the chimera-immunized animals showed a significant reduction in bacterial lung counts (P = 0.01) from that of the sham control group. Thus, a divalent S1 fragment CtxA2B chimera is an immunogenic antigen and can elicit a protective immunity.
Lee
Song F
SF
Department of Applied Oral Sciences, Faculty of Dentistry, Dalhousie University, Halifax, Nova Scotia, Canada B3H 3J5. song.lee@dal.ca
Halperin
Scott A
SA
Salloum
Danny F
DF
MacMillan
Ann
A
Morris
Annette
A
D000281:Administration, Intranasal; D000818:Animals; D001886:Bordetella pertussis / Q000276:immunology; D002772:Cholera Toxin / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005818:Genetic Engineering; D018928:Immunity, Mucosal; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D037342:Pertussis Toxin / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D010567:Pertussis Vaccine / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D012463:Saliva / Q000276:immunology; D014917:Whooping Cough / Q000517:prevention & control*
D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D010567:Pertussis Vaccine; D011993:Recombinant Fusion Proteins; D002772:Cholera Toxin; D037342:Pertussis Toxin