Identification and characterisation of Schizosaccharomyces pombe cyclophilin 3, a cyclosporin A insensitive orthologue of human USA-CyP.
We have identified nine cyclophilins encoded in the genome of the fission yeast Schizosaccharomyces pombe (Sp). Cyclophilin 3 is an orthologue of hUSA-CyP, which is associated with Prp4/Prp3 in the [U4/U6.U5] snRNP complex and Prp18, both of which are components of the pre-mRNA splicing machinery. PPIase assays have shown SpCyp3 and hUSA-CyP to have comparable activity and substrate specificity, but SpCyp3 has a reduced sensitivity to CsA correlating with a difference in the catalytic site. Prp3, Prp4 and Prp18 proteins exist in S. pombe and nuclear localisation of SpCyp3 has been shown, indicating conservation of function between hUSA-CyP and SpCyp3.
Pemberton
Trevor J
TJ
The School of Biological Sciences, University of Sussex, Falmer, East Sussex, BN1 9QG, Brighton, UK. t.j.pemberton@sussex.ac.uk
Rulten
Stuart L
SL
Kay
John E
JE
D000595:Amino Acid Sequence; D001483:Base Sequence; D021984:Cyclophilin A / Q000737:chemistry*; D021983:Cyclophilins / Q000737:chemistry*; D017931:DNA Primers; D004271:DNA, Fungal; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012568:Schizosaccharomyces / Q000737:chemistry* / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D004271:DNA, Fungal; D029702:Schizosaccharomyces pombe Proteins; D021984:Cyclophilin A; D021983:Cyclophilins; C479571:cyp3 protein, S pombe