Interleukin-2 induces peroxide production by primed normodense eosinophils of patients with asthma.
In this study we assessed, by flow cytometry, the effect of interleukin 2 (IL-2) on the oxidative burst of normodense eosinophils (Eos's) isolated from 15 patients with moderately severe extrinsic asthma and 17 controls. We found that IL-2 significantly induced peroxide (H2O2) production in normodense Eos's from patients with asthma on a time kinetics study. This rise was higher in patients with immunoglobulin E levels > 180 IU/mL versus normal immunoglobulin E values. The effect of IL-2 was partially blocked by using anti-Tac antibody. In contrast, IL-2 decreased H2O2 production in normodense Eos's from controls. Cell surface expression of CD25, CD122, CD132, and CD69 were also determined and no statistical differences were found between both groups. In conclusion, IL-2 is able to increase H2O2 production by normodense Eos's isolated from patients with asthma and it may contribute to bronchial epithelium damage and chronic inflammation.
Conesa
Angela
A
Instituto de Inmunología, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela.
Tassinari
Paolo
P
Aldrey
Oscar
O
Taylor
Peter
P
Bianco
Nicolas E
NE
De Sanctis
Juan B
JB
D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis / Q000187:drug effects; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis / Q000187:drug effects; D000954:Antigens, Surface / Q000096:biosynthesis / Q000187:drug effects; D001249:Asthma / Q000097:blood* / Q000503:physiopathology; D015415:Biomarkers / Q000097:blood; D002273:Carcinogens / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004396:Coloring Agents; D004804:Eosinophils / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007376:Interleukin-2 / Q000378:metabolism* / Q000494:pharmacology*; D037181:Lectins, C-Type; D008297:Male; D010545:Peroxides / Q000097:blood / Q000378:metabolism*; D017452:Receptors, IgG / Q000096:biosynthesis / Q000187:drug effects; D016897:Respiratory Burst / Q000187:drug effects / Q000502:physiology; D013268:Stimulation, Chemical; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014343:Trypan Blue; D014687:Venezuela; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D015415:Biomarkers; D002273:Carcinogens; D004396:Coloring Agents; D007073:Immunoglobulin E; D007376:Interleukin-2; D037181:Lectins, C-Type; D010545:Peroxides; D017452:Receptors, IgG; D013755:Tetradecanoylphorbol Acetate; D014343:Trypan Blue; D000099192:CD69 Antigens