title: Multiparameter flow cytometry in the diagnosis of a gynaecologic double tumor: a case report.
abstract: OBJECTIVE
  An uncommon clinical presentation of metastatic tumor will often lead to additional diagnostic examinations. The patient of the present study was known to have endometrial cancer which was thought to be limited to the endometrium. Three months postoperatively, she developed ascites due to spread of the tumor, which is rarely seen in low-stage endometrial cancer.
  
  
  METHODS
  Multiparameter flow cytometry using both cell phenotype information and DNA ploidy was performed.
  
  
  RESULTS
  Retrospectively, the patient was diagnosed as having a DNA-diploid epithelial tumor of the endometrium as well as a DNA-aneuploid epithelial tumor in the left fallopian tube. It was shown that 3 months after primary surgery she developed ascites caused by metastatic tumor from the primary fallopian tube cancer.
  
  
  CONCLUSIONS
  The complete diagnosis was made using multiparameter flow cytometry which, at present, is not routinely applied in gynecologic pathology.
authors.affiliation: Maaslandziekenhuis Sittard, Department of Obstetrics and Gynaecology, Research Institute Growth and Development GROW, Walramstraat 23, 6131 BK Sittard, Maastricht, The Netherlands. h.mertens@orbisconcern.nl
authors.forename: H J M M
authors.initials: HJ
authors.lastname: Mertens
authors.forename: M
authors.initials: M
authors.lastname: Nap
authors.forename: M P G
authors.initials: MP
authors.lastname: Leers
authors.forename: T H B
authors.initials: TH
authors.lastname: Salemans
mesh_terms: D000368:Aged; D000782:Aneuploidy; D001201:Ascites; D004247:DNA / Q000032:analysis; D004171:Diploidy; D016889:Endometrial Neoplasms / Q000175:diagnosis* / Q000235:genetics; D005185:Fallopian Tube Neoplasms / Q000175:diagnosis* / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D009362:Neoplasm Metastasis; D009378:Neoplasms, Multiple Primary / Q000175:diagnosis*; D011003:Ploidies
chemical_list: D004247:DNA