Multiplex PCR for detection of aminoglycoside resistance genes in enterococci.
A multiplex PCR procedure for detecting the aminoglycoside resistance genes aac(6')-Ie-aph(2")-Ia, aph(2")-Ib, aph(2")-Ic, aph(2")-Id, aph(3')-IIIa, and ant(4')-Ia was evaluated and found to determine accurately the presence of these genes in enterococci.
Vakulenko
Sergei B
SB
Division of Infectious Diseases, Department of Biochemistry and Molecular Biology, Wayne State University, Detroit, Michigan 48201, USA.
Donabedian
Susan M
SM
Voskresenskiy
Anatoliy M
AM
Zervos
Marcus J
MJ
Lerner
Stephen A
SA
Chow
Joseph W
JW
D000617:Aminoglycosides; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016983:Enterococcus / Q000187:drug effects* / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents