Detection of pap-, sfa- and afa-specific DNA sequences in Escherichia coli strains isolated from extraintestinal material.
P-fimbriae, S-fimbriae and AFA-adhesins are virulence factors responsible for adherence of Escherichia coli strains to extraintestinal host-cell surface. Detection of pap-, sfa- and afa-specific sequences performed by PCR revealed 74% pap+, 65% sfa+, and 8.3% afa+ strains in a group of 84 extraintestinal E. coli isolates. Detection in a group of fecal strains showed 29% pap+, 21% sfa+ and 4% afa+ strains. pap together with sfa were found as the most frequent combination (56%) among extraintestinal isolates probably due to localization of pap- and sfa-operons on a common pathogenicity island. The occurrence of afa-specific sequence among 56 urine strains was 11%, although no afa+ strain was detected among 28 gynecological isolates. No strains with detected adhesin operons were found among twenty (24%) extraintestinal E. coli strains.
Bogyiová
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E
Institute of Medical Microbiology, School of Medicine, Safárik University, 040 66 Kosice, Slovakia. bogyie@central.medic.upjs.sk
Kmetová
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M
Biros
E
E
Siegfried
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L
D018830:Adhesins, Escherichia coli / Q000235:genetics* / Q000502:physiology; D000328:Adult; D001422:Bacterial Adhesion / Q000502:physiology*; D002648:Child; D002675:Child, Preschool; D003556:Cystitis / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology; D005260:Female; D010861:Fimbriae, Bacterial / Q000502:physiology*; D005831:Genital Diseases, Female / Q000382:microbiology; D006801:Humans; D007223:Infant; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011704:Pyelonephritis / Q000382:microbiology; D014774:Virulence
D018830:Adhesins, Escherichia coli; D004269:DNA, Bacterial