title: Molecular cloning and characterization of mannan binding lectin C gene from Balb/c mice.
abstract: OBJECTIVE
  To obtain full-length cDNA encoding mouse mannan-binding lectin C(MBL-C) polypeptide.
  
  
  METHODS
  The cDNA encoding mouse MBL-C was isolated from Balb/c mouse liver cells by reverse transcriptase (RT)-PCR and inserted into pUC-T vector, and the encoding region structure of the DNA was analyzed to understand its evolutionary relationship with single human MBL homologues.
  
  
  RESULTS
  The amplified mouse MBL-C cDNA, which was 735 bp in length, encoded 245 amino acid residues, and its structural analysis showed 100% homology with published mouse MBL-C gene sequence and 71.4% homology with MBL gene of Chinese human.
  
  
  CONCLUSIONS
  The gene encoding Balb/c mouse MBL-C has been successfully isolated, which may facilitate further study of the innate immune functions of MBL molecule in vivo.
authors.affiliation: Department of Immunology, First Military Medical University, Guangzhou 510515, China. whwnet@fimmu.com
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Wang
authors.forename: Zheng-liang
authors.initials: ZL
authors.lastname: Chen
authors.forename: Da-ming
authors.initials: DM
authors.lastname: Zuo
authors.forename: Xiao
authors.initials: X
authors.lastname: Lu
authors.forename: Xin-pei
authors.initials: XP
authors.lastname: Yu
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis*; D005260:Female; D006801:Humans; D037601:Mannose-Binding Lectin / Q000031:analogs & derivatives* / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018076:DNA, Complementary; D037601:Mannose-Binding Lectin; C445639:Mbl2 protein, mouse