The energetics of specific binding of AT-hooks from HMGA1 to target DNA.
The interaction of the second and third AT-hooks of HMGA1 (formerly HMGI/Y), which bind selectively in the minor groove of an AT-rich DNA sequence, was studied at different temperatures and ionic strengths by spectropolarimetry, spectrofluorimetry, isothermal titration calorimetry and differential scanning calorimetry. The data show that binding of the ten amino acid core element of the two AT-hooks, which penetrates deep into the minor groove, is entropically driven: both the entropy and enthalpy of association of the peptides to the target DNA are positive up to 50 degrees C. The seven amino acid extension of the core in the second AT-hook, which extends out from the minor groove and loops over the phosphodiester backbone, adds a substantial negative enthalpic component into the binding of the 17 residue DBD2 peptide to DNA that corresponds in magnitude to the enthalpy of formation of two hydrogen bonds. The ionic strength dependence of the association constant allowed an estimation of the electrostatic component of binding and, by subtraction, the contribution of the non-electrostatic component, which results from dehydration of the contacting surfaces and makes up almost 70% of the total energy of complex formation. The exceptionally large positive entropy and enthalpy of association of the core AT-hook peptides with target DNA suggest that the water, which is removed from the minor groove of DNA upon binding, is in a highly ordered state. Acetylation of the lysine residue in the second AT-hook, which corresponds to Lys65 of HMGA1, has little effect on the DNA binding; so it appears that repression of the hIFNbeta gene, which follows this modification, is not a direct result of the abrogation of DNA binding.
Dragan
Anatoly I
AI
Department of Biology, Johns Hopkins University, 144 Mudd Hall, 3400 N Charles Street, Baltimore, MD 21218-2685, USA.
Liggins
John R
JR
Crane-Robinson
Colyn
C
Privalov
Peter L
PL
D001665:Binding Sites; D002152:Calorimetry, Differential Scanning; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004734:Energy Metabolism; D019277:Entropy; D025741:HMGA1a Protein / Q000737:chemistry* / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D006860:Hydrogen Bonding; D008024:Ligands; D008239:Lysine / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013816:Thermodynamics
D008024:Ligands; D010446:Peptide Fragments; D025741:HMGA1a Protein; D004247:DNA; D008239:Lysine