Practical considerations in refolding proteins from inclusion bodies.
Refolding of proteins from inclusion bodies is affected by several factors, including solubilization of inclusion bodies by denaturants, removal of the denaturant, and assistance of refolding by small molecule additives. We will review key parameters associated with (1) conformation of the protein solubilized from inclusion bodies, (2) change in conformation and flexibility or solubility of proteins during refolding upon reduction of denaturant concentration, and (3) the effect of small molecule additives on refolding and aggregation of the proteins.
Tsumoto
Kouhei
K
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai, Japan.
Ejima
Daisuke
D
Kumagai
Izumi
I
Arakawa
Tsutomu
T
D002479:Inclusion Bodies / Q000737:chemistry*; D011487:Protein Conformation / Q000187:drug effects; D017510:Protein Folding; D020673:Protein Renaturation / Q000187:drug effects; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D012995:Solubility / Q000187:drug effects
D011506:Proteins