title: Animated documentation of the filaria dance sign (FDS) in bancroftian filariasis.
abstract: BACKGROUND: Ultrasonography is able to detect adult Wuchereria bancrofti worms in scrotal lymphatic vessels of infected men on account of the characteristic pattern of adult worm movements, known as the filarial dance sign. Furthermore, the technique is able to delineate associated pathology, such as hydrocoele and lymphoedema, which can be diagnosed in early stages. Ultrasonography is also useful in the assessment of macrofilaricidal effects of antifilarial medication.The purpose of this study was to evaluate the usefulness of scrotal ultrasonography, in combination with a new method of digital documentation, in men infected with Wuchereria bancrofti. METHODS: Ultrasonography of the scrotal areas was carried out in 33 male patients from an endemic area in Ghana using a hand-carried ultrasound system and a linear array transducer at 7.5 MHz. Wuchereria bancrofti infection was also assessed by quantification of night blood microfilaraemia and semi-quantitative detection of circulating filarial antigen. Ultrasound findings were documented by print outs and by Digital Video sequences directly exported from the ultrasound machine which were edited in Final Cut Pro 3ledR; and exported, using QuickTimecircledR; Pro, as MPEG-1 video. RESULTS: Worm nests, i.e. dilated lymphatic vessels with the characteristic movement patterns of worms, were found in all patients, and typical examples of larger as well as smaller nests are presented through MPEG-1 video in b- and m-modes as well as Colour Doppler and Pulse Wave Doppler images. CONCLUSION: In this study, the filarial dance sign is being made available on the Internet to readers through MPEG-1 video. This method allows for demonstration of movement patterns rather than static images. In addition, the pathologic ultrasonographic signs of filariasis can be rapidly relayed over great distances and may be helpful to other investigators or clinicians in the diagnosis of patients infected with Wuchereria bancrofti.
authors.affiliation: Department of Helminthology; Bernhard-Nocht-Institute for Tropical Medicine, 20359, Hamburg, Germany. hoerauf@bni.uni-hamburg.de
authors.forename: Sabine
authors.initials: S
authors.lastname: Mand
authors.forename: Yeboah
authors.initials: Y
authors.lastname: Marfo-Debrekyei
authors.forename: Matthias
authors.initials: M
authors.lastname: Dittrich
authors.forename: Kerstin
authors.initials: K
authors.lastname: Fischer
authors.forename: Ohene
authors.initials: O
authors.lastname: Adjei
authors.forename: Achim
authors.initials: A
authors.lastname: Hoerauf