[Treatment of burn surfaces by proteinases: mathematical description of an enzyme distribution].
The process of penetration of a proteolytic enzyme applied to the surface of burn wound into the depth of necrotic tissue was considered. The model approximation describes three factors by a series of mathematical equations: inward-directed enzyme diffusion, counter-flow filtration of interstitial fluid (exudates), and irreversible inactivation of the enzyme by specific inhibitors present in exudates. According to the model, a quasi-stationary distribution of enzymatic activity through the thickness of the necrotic layer is achieved within 3 h and persists as long as the enzyme concentration on the wound surface is constant. The enzyme activity diminishes linearly from the wound surface to the mid-part of the necrotic layer. No enzyme activity is retained in the inner mid-part of the necrotic layer completely protected by the prevalent inhibitor. The ratio of enzyme concentration on the wound surface to inhibitor concentration in the interstitial fluid is the same as the ratio of the depth of active enzyme area to the depth of the inhibitor-protected area through the necrotic layer. The dynamics of accumulation of the active enzyme in the necrotic zone and the rate of enzyme inactivation in the wound by inhibitors were described by formulas applicable for practical purposes.
Khalili
A S
AS
Lomonosov Moscow State University, Vorob'evy Gory, Moscow, 119899 Russia.
Domogatskiĭ
S P
SP
Blizniukov
O P
OP
Ruuge
E K
EK
D002056:Burns / Q000378:metabolism* / Q000473:pathology; D004058:Diffusion; D008954:Models, Biological; D009336:Necrosis; D011480:Protease Inhibitors / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism / Q000493:pharmacokinetics*; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D014945:Wound Healing
D011480:Protease Inhibitors; D012697:Serine Endopeptidases