title: Cu,Zn-superoxide dismutase of Saccharomyces cerevisiae is required for resistance to hyperosmosis.
abstract: Here we analyzed the role of the antioxidant response in Saccharomyces cerevisiae adaptation to hyperosmotic stress. We show that Cu,Zn-superoxide dismutase (SOD1) plays a fundamental role in this adaptation process since under hyperosmosis SOD1 mutants lead to high protein oxidation levels and show a sensitive phenotype, which is reversed by the addition of N-acetylcysteine to the medium. Pretreatment with MnCl(2), a superoxide scavenger, improves the survival of the sod1 strain upon hyperosmosis. Additionally, we show that upon hyperosmotic shock there is a small and transient increase in SOD1 transcript levels, regulated by the protein kinase A-cAMP and SKN7 pathways.
authors.affiliation: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Apdo. Postal 510-3, Cuernavaca 62250, Morelos, Mexico.
authors.forename: Adriana
authors.initials: A
authors.lastname: Garay-Arroyo
authors.forename: Fernando
authors.initials: F
authors.lastname: Lledías
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Hansberg
authors.forename: Alejandra A
authors.initials: AA
authors.lastname: Covarrubias
mesh_terms: D000222:Adaptation, Physiological; D000975:Antioxidants / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D009154:Mutation; D009997:Osmotic Pressure; D010084:Oxidation-Reduction; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D013482:Superoxide Dismutase