Immunohistochemical analysis of Fas protein expression in hypertrophic scar and keloid.
OBJECTIVE
To find an efficient diagnostic method of hypertrophic scar and keloid.


METHODS
Immunohistochemistry was employed to detect the expression and distribution of Fas protein in keloids, hypertrophic scars and their surrounding normal skins. Image analysis was performed to quantitatively compare the differences of the protein expression in these tissues.


RESULTS
The expression level of Fas antigen was much higher in the keloids and their surrounding normal skins than in hypertrophic scars, and no significant difference in the expression level between the former 2 tissues was found. In hypertrophic scars, however, much lower Fas protein expression was detected in comparison with that in their surrounding normal skins(P < 0.01).


CONCLUSIONS
Immunohistochemical detection of Fas protein is a simple method that is well applicable in the differential diagnosis of pathological scars.
Lu
Feng
F
Department of Plastic Surgery, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Gao
Jian-hua
JH
Li
Xiao-jian
XJ
D017439:Cicatrix, Hypertrophic / Q000175:diagnosis* / Q000378:metabolism; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D007627:Keloid / Q000175:diagnosis* / Q000378:metabolism; D019014:fas Receptor / Q000032:analysis*
D019014:fas Receptor