Polyunsaturated hydrocarbons in the hemolymph: biosynthetic precursors of epoxy pheromones of geometrid and arctiid moths.
Female moths of many species in Geometridae, Arctiidae and some other macrolepidopteran families produce epoxy pheromones, which are probably derived from polyunsaturated hydrocarbons. In order to understand a biosynthetic site, hemolymph from both sexes of two geometrid species, Ascotis selenaria cretacea and Hemerophila artilineata, and one arctiid species, Spilosoma imparilis, was shaken with n-hexane and the solvent extracts were analyzed by GC-MS. Each extract of the female hemolymph sex-specifically included polyunsaturated hydrocarbons corresponding to the pheromonal epoxy components in addition to many saturated hydrocarbons, but no epoxy compounds were detected in it. Based on this analysis, deuterated polyunsaturated hydrocarbons were injected into the abdomens of two geometrid females, and the labeled epoxy components were successfully yielded from the pheromone glands. This result indicated that the polyunsaturated hydrocarbons occurring in the female hemolymph were direct pheromone precursors, which might be produced outside the pheromone gland, probably in oenocytes associated with abdominal epidermal cells or in the fat body, and transported to the pheromone gland via the hemolymph for their epoxydation and emission into the atmosphere.
Wei
W
W
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
Miyamoto
T
T
Endo
M
M
Murakawa
T
T
Pu
G-Q
GQ
Ando
T
T
D000818:Animals; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D006458:Hemolymph / Q000737:chemistry*; D006838:Hydrocarbons / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D009036:Moths / Q000145:classification / Q000378:metabolism*; D012724:Sex Attractants / Q000096:biosynthesis* / Q000737:chemistry; D013045:Species Specificity
D006838:Hydrocarbons; D012724:Sex Attractants