Perils of immunohistochemistry: variability in staining specificity of commercially available COX-2 antibodies on human colon tissue.
We assessed the immunohistochemical (IHC) staining patterns of three commercially available COX-2 antibodies in human tissues. The location of positive stain in sequential serial 4-mu sections of formalin fixed, paraffin embedded tissue differed considerably. Staining patterns ranged from diffuse cytoplasmic to occasional perinuclear staining. Thus, marked variability in staining results from use of different antibodies, making it important to consider the antibody used when comparing reports of COX-2 staining from different investigators.
Garewal
Harinder
H
Hematology-Oncology Section, Tucson Veterans Affairs Hospital, 3601 S. 6th Ave., Tucson, Arizona 85723, USA.
Ramsey
Lois
L
Fass
Ronnie
R
Hart
Nancy K
NK
Payne
Claire M
CM
Bernstein
Harris
H
Bernstein
Carol
C
D000911:Antibodies, Monoclonal; D003106:Colon / Q000378:metabolism*; D051546:Cyclooxygenase 2; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000276:immunology*; D008565:Membrane Proteins; D010544:Peroxidases / Q000276:immunology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D008565:Membrane Proteins; D010544:Peroxidases; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases