Human coronavirus 229E: receptor binding domain and neutralization by soluble receptor at 37 degrees C.
Truncated human coronavirus HCoV-229E spike glycoproteins containing amino acids 407 to 547 bound to purified, soluble virus receptor, human aminopeptidase N (hAPN). Soluble hAPN neutralized the infectivity of HCoV-229E virions at 37 degrees C, but not 4 degrees C. Binding of hAPN may therefore trigger conformational changes in the viral spike protein at 37 degrees C that facilitate virus entry.
Breslin
Jamie J
JJ
Department of Microbiology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA.
Mørk
Irene
I
Smith
M K
MK
Vogel
Lotte K
LK
Hemmila
Erin M
EM
Bonavia
Aurelio
A
Talbot
Pierre J
PJ
Sjöström
Hans
H
Norén
Ove
O
Holmes
Kathryn V
KV
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D018826:CD13 Antigens / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D028941:Coronavirus 229E, Human / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D000085064:Receptors, Coronavirus; D011991:Receptors, Virus / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017384:Sequence Deletion; D012995:Solubility; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology
D008562:Membrane Glycoproteins; D000085064:Receptors, Coronavirus; D011991:Receptors, Virus; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D018826:CD13 Antigens