[Improvement of cardiomyocyte isolation for different purposes].
To improve the methods of isolating single ventricular myocytes for purposes of different experiments.



The cardiac ventricular myocytes were isolated enzymatically by Langendorff perfusion technique at constant flow rate. The patch clamp whole-cell recording, a spectrofluorometric method and a video  tracking system were used to verify the basic electrophysiological properties, intracellular calcium transient and cell contraction of the single myocytes.



The resting membrance potential of the myocyte was (-74.06+/-4.54)mV. The whole-cell sodium, potassium and calcium currents, intracellular calcium transients and contractile properties were stable and typical.



The myocytes obtained by adjusting perfusion parameters are well suited for electrophysiological recording, intracellular calcium and single cell contraction measurements.
Cao
Chun-Mei
CM
Department of Physiology, College of Medical Sciences, Zhejiang University  Hangzhou 310031, China.
Zhang
Xiong
X
Chen
Ying-Ying
YY
D000200:Action Potentials; D000818:Animals; D015220:Calcium Channels / Q000502:physiology; D002469:Cell Separation / Q000379:methods*; D005260:Female; D006352:Heart Ventricles / Q000166:cytology*; D008297:Male; D032383:Myocytes, Cardiac / Q000502:physiology*; D015221:Potassium Channels / Q000502:physiology; D051381:Rats
D015220:Calcium Channels; D015221:Potassium Channels