Expressional changes of ganglioside GM3 during ovarian maturation and early embryonic development in db/db mice.
Diabetes and obesity cause abnormal development of reproductive processes in a variety of species, but the mechanisms that underlie this effect have not been fully elucidated. This study examined the expressional changes of ganglioside GM3 during ovarian maturation, in vitro fertilization (IVF) and early embryonic development in diabetic/obese db/db mice. In high-performance thin-layer chromatography studies, GM3 expression was conspicuously low in the ovaries of db/db mice compared to non-diabetic db/+ mice. Signal detected by anti-GM3 monoclonal antibody was greatly reduced in the primary, secondary and graffian follicles of db/db mice compared to control mice. Results from IVF with ova and sperm from db/db mice showed that GM3 expression during early embryonic development was obviously decreased compared to db/+ mice. This study also elucidated the effects of high glucose (20 and 30 mm) on early embryonic development in ICR strain mice. High glucose caused a decrease in GM3 expression during early embryonic development. Taken together, the results of this study indicate decreased GM3 expression during ovarian maturation and embryonic development of db/db mice, suggesting that alteration of ganglioside expression induced by the diabetic condition may be implicated in the abnormal follicular embryonic development.
Kwak
Dong Hoon
DH
College of Natural Sciences and Research Institute for Basic Sciences of Wonkwang University, 344-2 Shinyong-dong, Iksan, Jeonbuk 570-749, Korea.
Jung
Kyu Yong
KY
Lee
Young Choon
YC
Choo
Young Kug
YK
D000818:Animals; D002855:Chromatography, Thin Layer; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D004195:Disease Models, Animal; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D005679:G(M3) Ganglioside / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D010053:Ovary / Q000378:metabolism*
D005679:G(M3) Ganglioside