A novel alpha-helical protein, specific to and highly conserved in plants, is associated with the nuclear matrix fraction.
A cDNA for a novel plant protein was isolated from tomato. Nuclear Matrix Protein 1 (NMP1) is a ubiquitously expressed 36 kDa protein, which has no homologues in animals and fungi, but is highly conserved among flowering and non-flowering plants, including gymnosperms, moss, and the liverwort Marchantia polymorpha. NMP1 is predominantly alpha-helical with multiple stretches of short amphipathic regions. Cell fractionation, immunofluorescence, and GFP localization experiments showed that NMP1 is located both in the cytoplasm and nucleus and that the nuclear fraction is associated with the nuclear matrix. NMP1 is a candidate for a plant-specific structural protein with a function both in the nucleus and cytoplasm.
Rose
Annkatrin
A
Plant Biotechnology Center and Department of Plant Biology, Ohio State University, Columbus, OH 43210, USA.
Gindullis
Frank
F
Meier
Iris
I
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000187:drug effects; D018551:Solanum lycopersicum / Q000235:genetics*; D008969:Molecular Sequence Data; D015530:Nuclear Matrix / Q000378:metabolism*; D034521:Nuclear Matrix-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques
D018076:DNA, Complementary; D034521:Nuclear Matrix-Associated Proteins; D010940:Plant Proteins