mecA-blaZ corepressors in clinical Staphylococcus aureus isolates.
The presence and nucleotide sequences of the two mecA repressors, mecI and blaI, were assessed in 73 clinical Staphylococcus aureus isolates. Isolates with mecI mutations were grouped into unique clonal types based on their spa nucleotide repeat patterns. Forty-three of the 45 (96%) isolates with mutant mecI or with a deletion of mecI contained blaI, while blaI was present in only 21 of 28 (78%) isolates with wild-type mecI (P < 0.05). Among 22 additional isolates that did not contain blaI, all had wild-type mecI sequences. We conclude that oxacillin-resistant S. aureus must have at least one of the two functional mecA regulators.
Rosato
Adriana E
AE
Department of Medicine, Medical College of Virginia Campus of Virginia Commonwealth University, Richmond, Virginia 23298, USA.
Kreiswirth
Barry N
BN
Craig
William A
WA
Eisner
William
W
Climo
Michael W
MW
Archer
Gordon L
GL
D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000235:genetics*; D006602:Hexosyltransferases; D006801:Humans; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics*; D010068:Oxacillin / Q000494:pharmacology*; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D012097:Repressor Proteins / Q000032:analysis*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
D001426:Bacterial Proteins; D002352:Carrier Proteins; C101640:MecI protein, Staphylococcus; D046915:Penicillin-Binding Proteins; D012097:Repressor Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D001618:beta-Lactamases; D010068:Oxacillin