Positive and negative regulators for neuronal BC1 RNA transcription by RNA polymerase III are possible members of the RNA polymerase II transcription system.
Neuronal cell-specific BC1 RNA is a unique RNA polymerase III (Pol III) transcript. The transcription is controlled by an activator E2 site and by BCRE, a repressor element, in response to neuronal activity. BC1 RNA is localized to dendritic domains as ribonucleoprotein particles, and it has been suggested to play a functional role in translational regulation of dendritic mRNAs. In the present study, using a luciferase assay in NG108-15 cells, we found that the positive and negative regulators for BC1 RNA transcription can also function in the Pol II transcription system. Our results suggest that the neuronal activity-dependent expression of BC1 RNA by Pol III and a subset of neuronal mRNAs by Pol II may be simultaneously controlled by the E2 site and BCRE, as well as their binding proteins.
Kobayashi
Shunsuke
S
Department of Biochemistry, College of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi-shi, Chiba 274-8555, Japan.
Kamo
Sakurako
S
Agui
Keisuke
K
Funakoshi
Tomoko
T
Anzai
Kaijiro
K
D000818:Animals; D001665:Binding Sites / Q000235:genetics; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D003712:Dendrites / Q000378:metabolism; D005809:Genes, Regulator / Q000235:genetics*; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D011485:Protein Binding / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics; D012319:RNA Polymerase II / Q000235:genetics / Q000378:metabolism*; D012320:RNA Polymerase III / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020733:RNA, Small Cytoplasmic / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D012097:Repressor Proteins / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics
C117734:BC1 RNA; D012333:RNA, Messenger; D020733:RNA, Small Cytoplasmic; D012097:Repressor Proteins; D012319:RNA Polymerase II; D012320:RNA Polymerase III