title: Does sucrose influence the properties of DMPC vesicles?
abstract: Small-angle neutron and X-ray scattering, dynamic light scattering, X-ray diffraction coupled with differential scanning calorimetry, and Raman spectroscopy were applied to investigate unilamellar (ULVs) and multilamellar (MLVs) dimyristoylphosphatidylcholine (DMPC) vesicles in aqueous sucrose solutions with sucrose concentrations from 0 to 60% w/w. In case of ULVs, the addition of sucrose decreases the polydispersity of vesicle population. A minimum value of polydispersity was found at 20% sucrose. For sucrose concentration from 0 to 35% oligolamellar vesicles in the ULV population have a minimum presence. Vesicles with 5-10% sucrose exhibit the best stability in time. For the case of MLVs, sucrose influences the temperature of the phase transitions, but the internal membrane structure remains unchanged.
authors.affiliation: Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research (JINR), 141980 Dubna, Moscow Region, Russia. kiselev@nf.jinr.ru
authors.forename: M A
authors.initials: MA
authors.lastname: Kiselev
authors.forename: S
authors.initials: S
authors.lastname: Wartewig
authors.forename: M
authors.initials: M
authors.lastname: Janich
authors.forename: P
authors.initials: P
authors.lastname: Lesieur
authors.forename: A M
authors.initials: AM
authors.lastname: Kiselev
authors.forename: M
authors.initials: M
authors.lastname: Ollivon
authors.forename: R
authors.initials: R
authors.lastname: Neubert
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D046911:Macromolecular Substances; D008560:Membrane Fluidity; D021962:Membrane Microdomains / Q000737:chemistry*; D008968:Molecular Conformation; D013059:Spectrum Analysis, Raman; D013395:Sucrose / Q000737:chemistry*; D013696:Temperature; D014867:Water / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D046911:Macromolecular Substances; D014867:Water; D015060:1,2-Dipalmitoylphosphatidylcholine; D013395:Sucrose