Interactions of lactic acid bacteria with human intestinal epithelial cells: effects on cytokine production.
As a participant in the mucosal immune response, the intestinal epithelial cell must respond to a variety of stimuli, including lactic acid bacteria (LAB) consumed in the diet. The objective of this study was to compare the abilities of several strains of LAB to modulate cytokine secretion by human intestinal epithelial cell (IEC) line HT-29. Certain strains of Lactobacillus rhamnosus, Lactobacillus delbrueckii, and Lactobacillus acidophilus suppressed the production of the chemokine RANTES by stimulated HT-29 IEC, although the magnitude of this suppression varied depending on the nature of the bacterial growth medium. Similarly, specific strains showed growth condition-dependent suppression of HT-29 interleukin-8 (IL-8) production. Strain-dependent effects were also seen for the suppression of tumor necrosis factor alpha (TNF-alpha) and transforming growth factor beta (TGF-beta) production. The binding of several of these bacterial strains to the HT-29 cell line was also examined. Different strains were found to have differing abilities to interact with IEC, with L. rhamnosus R0011 being the strain that generally had the most extensive effects on HT-29 cytokine production and also bound to HT-29 IEC most effectively. Modulation of IEC cytokine production has the potential to profoundly affect the mucosal microenvironment, influencing the immune response to pathogens and other ingested antigens.
Wallace
Timothy D
TD
Department of Biology, Acadia University, Wolfville, Nova Scotia, Canada B0P 1X0.
Bradley
Shannon
S
Buckley
Nicole D
ND
Green-Johnson
Julia M
JM
D001422:Bacterial Adhesion; D001681:Biological Assay; D016207:Cytokines / Q000096:biosynthesis*; D019073:HT29 Cells; D006801:Humans; D018928:Immunity, Mucosal; D016209:Interleukin-8 / Q000096:biosynthesis; D007778:Lactobacillus / Q000276:immunology* / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D016207:Cytokines; D016209:Interleukin-8; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha