title: Effect of Helicobacter pylori infection on epidermal growth factor receptor (EGFR) expression and cell proliferation of gastric epithelial mucosa: correlation to macroscopic and microscopic diagnosis.
abstract: Our aim was to compare the expression of EGFR and proliferative cell nuclear antigen (PCNA) in different histological and endoscopic diagnostic groups, in cases of Helicobacter pylori infection, in vivo. Paraffin embedded human gastric biopsy samples (86) were analysed by EGFR and PCNA immunohistochemistry and classified both on the basis of histology and endoscopic findings. In normal epithelia (NE), a positive correlation was found between PCNA and EGFR and in H. pylori-negative gastritis with and without intestinal metaplasia (P < 0.01). On the other hand, a negative correlation was detected between the two immunohistochemical findings in H. pylori-associated gastritis with intestinal metaplasia (HPGIM) and in the atrophic gastritis (AG) group. In HPGIM the percentage of EGFR-positive cells was significantly lower (32.4 +/- 30.4) when compared to either the NE (50.3 +/- 23.7) or H. pylori-negative gastritis with intestinal metaplasia (HNGIM) (48.3 +/- 23.7). In AG, EGFR was significantly lower when compared to the NE (P < 0.05). Based on the endoscopic findings, a significant decrease of EGFR expression was found in gastric ulcer cases as compared to NE, gastritis or erosion cases (P < 0.01). PCNA showed no significant alterations between the NE and gastritis, AG groups. The presence of H. pylori has an inverse effect on PCNA and EGFR expression in HPGIM.
authors.affiliation: Semmelweis University, Faculty of Medicine, Second Department of Medicine, Budapest, Hungary.
authors.forename: Agnes
authors.initials: A
authors.lastname: Ruzsovics
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Unger
authors.forename: Bela
authors.initials: B
authors.lastname: Molnar
authors.forename: Laszlo
authors.initials: L
authors.lastname: Pronai
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Tulassay
mesh_terms: D015415:Biomarkers / Q000032:analysis; D002455:Cell Division; D004848:Epithelium / Q000382:microbiology / Q000473:pathology; D066246:ErbB Receptors / Q000032:analysis* / Q000378:metabolism*; D005753:Gastric Mucosa / Q000382:microbiology* / Q000473:pathology; D005756:Gastritis / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D016481:Helicobacter Infections / Q000378:metabolism* / Q000473:pathology; D016480:Helicobacter pylori; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D016612:Paraffin Embedding; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis
chemical_list: D015415:Biomarkers; D018809:Proliferating Cell Nuclear Antigen; D066246:ErbB Receptors