title: Electrochemical protein chip with arrayed immunosensors with antibodies immobilized in a plasma-polymerized film.
abstract: An electrochemical protein chip was microfabricated. A thin-film three-electrode system, including an array of 36 platinum working electrodes, a set of thin-film Ag/AgCl electrodes, and platinum auxiliary electrodes, was integrated on a glass substrate. Capture antibodies were immobilized in a 4.5-nm-thick double layer of a hexamethyldisiloxane plasma-polymerized film. Because of their highly cross-linked network structure, the capture antibodies could be firmly immobilized. No nonspecific adsorption was observed during a series of procedures to detect target proteins, and electrochemical cross talk between neighboring sites was negligible. The sandwich immunoassay was conducted on a single chip using model proteins, alpha-1-fetoprotein and beta2-microglobulin. A distinct current increase following the oxidation of hydrogen peroxide produced by the enzymatic reaction of glucose oxidase was observed, which indicates that the capture proteins could actually bind the target proteins. Two kinds of protein were detected independently on multiple sites with respective capture antibodies.
authors.affiliation: Institute of Materials Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Kojima
authors.forename: Atsunori
authors.initials: A
authors.lastname: Hiratsuka
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Suzuki
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Yano
authors.forename: Kazunori
authors.initials: K
authors.lastname: Ikebukuro
authors.forename: Isao
authors.initials: I
authors.lastname: Karube
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D007120:Immunochemistry / Q000295:instrumentation*; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D008838:Microcomputers; D011506:Proteins / Q000737:chemistry*
chemical_list: D000906:Antibodies; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D011506:Proteins