A non-redundant microarray of genes for two related bacteria.
A microarray with sequences from the annotated open reading frames (ORFs) in Salmonella enterica subspecies 1, serovar Typhimurium was supplemented with annotated chromosomal ORFs from serovar Typhi that are divergent from Typhimurium (>10% DNA sequence divergence). This non- redundant array was used to (i) measure changes in gene copy number in DNA from actively growing versus stationary Typhi and (ii) to reveal the transcriptional response of Typhi to peroxide, a stress similar to that experienced when they are phagocytosed by macrophages. In S.enterica subspecies 1, pairs of genomes differ in the presence or absence of approximately 10% of their genes. An array twice the size of that needed to cover all ORFs for one genome could carry close homologs of all the ORFs for 10 genomes. Non-redundant DNA arrays could be constructed for any group of closely related organisms that differ by the presence and absence of a few genes.
Porwollik
Steffen
S
Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121, USA.
Frye
Jonathan
J
Florea
Liliana D
LD
Blackmer
Felisa
F
McClelland
Michael
M
D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010545:Peroxides / Q000494:pharmacology; D019779:Salmonella enterica / Q000187:drug effects / Q000235:genetics*; D013045:Species Specificity
D010545:Peroxides