title: Desulfovibrio hydrothermalis sp. nov., a novel sulfate-reducing bacterium isolated from hydrothermal vents.
abstract: Mesophilic, hydrogenotrophic, sulfate-reducing bacteria were isolated from a deep-sea hydrothermal chimney sample collected at 13 degrees N on the East-Pacific Rise at a depth of 2,600 m. Two strains (BL5 and H9) were found to be phylogenetically similar to Desulfovibrio profundus (similarity >99%), whereas two other strains (H1 and AM13T) were found to be phylogenetically distinct (similarity 96.4%) from Desulfovibrio zosterae, their closest relative. Strain AM13T was characterized further. It was a barophilic, Gram-negative, non-sporulating, motile, vibrio-shaped or sigmoid bacterium possessing desulfoviridin. It grew at temperatures ranging from 20 to 40 degrees C, with an optimum at 35 degrees C in the presence of 2.5% NaCl. The pH range for growth was 6.7-8.2 with an optimum around 7.8. Strain AM13T utilized H2/CO2, lactate, formate, ethanol, choline and glycerol as electron donors. Electron acceptors were sulfate, sulfite and thiosulfate, but not elemental sulfur or nitrate. The G + C content of DNA was 47 mol%. Strain AM13T (= DSM 14728T = CIP107303T) differed from D. zosterae not only phylogenetically, but also genomically (DNA-DNA reassociation value between the two bacteria was 23.8%) and phenotypically. This isolate is therefore proposed as the type strain of a novel species of the genus Desulfovibrio, Desulfovibrio hydrothermalis sp. nov.
authors.affiliation: IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 avenue de la Méditerranée, 13288 Marseille cedex 09, France.
authors.forename: D
authors.initials: D
authors.lastname: Alazard
authors.affiliation: Laboratoire de Microbiologie Marine, CNRS-INSU-UMR 6117, Université de la Méditerranée, Marseille Luminy, France.
authors.forename: S
authors.initials: S
authors.lastname: Dukan
authors.affiliation: IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 avenue de la Méditerranée, 13288 Marseille cedex 09, France.
authors.forename: A
authors.initials: A
authors.lastname: Urios
authors.affiliation: IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 avenue de la Méditerranée, 13288 Marseille cedex 09, France.
authors.forename: F
authors.initials: F
authors.lastname: Verhé
authors.affiliation: IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 avenue de la Méditerranée, 13288 Marseille cedex 09, France.
authors.forename: N
authors.initials: N
authors.lastname: Bouabida
authors.affiliation: Laboratoire de Microbiologie Marine, CNRS-INSU-UMR 6117, Université de la Méditerranée, Marseille Luminy, France.
authors.forename: F
authors.initials: F
authors.lastname: Morel
authors.affiliation: IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 avenue de la Méditerranée, 13288 Marseille cedex 09, France.
authors.forename: P
authors.initials: P
authors.lastname: Thomas
authors.affiliation: IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 avenue de la Méditerranée, 13288 Marseille cedex 09, France.
authors.forename: J-L
authors.initials: JL
authors.lastname: Garcia
authors.affiliation: IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 avenue de la Méditerranée, 13288 Marseille cedex 09, France.
authors.forename: B
authors.initials: B
authors.lastname: Ollivier
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D003901:Desulfovibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006358:Hot Temperature; D006874:Hydrostatic Pressure; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S