The IntP C-terminal segment is not required for excision of bacteriophage Mx8 from the Myxococcus xanthus chromosome.
During lysogenization of myxophage Mx8, phage DNA can be integrated into the attB site of the Myxococcus xanthus chromosome through site-specific recombination. We previously demonstrated that the Mx8 attP site is located within the coding sequence of the Mx8 intP gene. Hence, the integration of Mx8 into the M. xanthus chromosome results in the conversion of the 112-amino-acid C-terminal segment of the IntP protein into a 13-amino-acid C-terminal segment of a new protein, IntR. To examine whether IntR is active for Mx8 excision, we have constructed a series of plasmids carrying various lengths of the intP-attP or intR-attR regions as well as the lacZ gene. The integrated Mx8 was excised at a high frequency, indicating that IntR is active for the excision. For Mx8 excision, a gene designated xis was shown to be required in addition to intR.
Tojo
Nobuki
N
Department of Biology, Tokyo Metropolitan University, Minamiohsawa, Hachioji-shi, Tokyo 192-0397, Japan.
Komano
Teruya
T
D001435:Bacteriophages / Q000235:genetics*; D001665:Binding Sites / Q000235:genetics; D002876:Chromosomes, Bacterial; D004254:DNA Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism; D008242:Lysogeny; D016941:Myxococcus xanthus / Q000235:genetics / Q000821:virology*; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
C100962:IntP protein, Bacteriophage Mx8; D010446:Peptide Fragments; D014764:Viral Proteins; D004254:DNA Nucleotidyltransferases; C063911:excisionase; D004720:Endonucleases