Cytoskeletal requirements for hepatitis C virus (HCV) RNA synthesis in the HCV replicon cell culture system.
Hepatitis C virus (HCV) induces microtubule aggregates in infected hepatocytes. To determine if cytoskeletal elements are important for HCV RNA synthesis, we examined the effect of cytoskeleton inhibitors on HCV replicon transcription in Huh7 cells. The data demonstrate that HCV replication complex-mediated RNA synthesis requires microtubule and actin polymerization.
Bost
Anne G
AG
Infectious Diseases Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285-0438, USA.
Venable
Daryl
D
Liu
Lifei
L
Heinz
Beverly A
BA
D000199:Actins / Q000378:metabolism; D002460:Cell Line; D003599:Cytoskeleton / Q000821:virology; D017930:Genes, Reporter; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008870:Microtubules / Q000821:virology; D020871:RNA Stability; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics*; D012093:Replicon; D014779:Virus Replication
D000199:Actins; D012367:RNA, Viral; D008156:Luciferases