In situ detection of TGF betas, TGF beta receptor II mRNA and telomerase activity in rat cholangiocarcinogenesis.
OBJECTIVE
Initial report on the in situ examination of the mRNA expression of transforming growth factor betas (TGFbetas), TGFbeta type II receptor (TbetaRII) and telomerase activity in the experimental rat liver tissue during cholangiocarcinogenesis.


METHODS
Rat liver cholangiocarcinogenesis was induced by 3'-methyl 4-dimethylazobenzene (3'Me-DAB). In situ hybridization was used to examine the TGFbetas) and TGFbeta type II receptor (TbetaRII) mRNA, in situ TRAP was used to check the telomerase activity in the tissue samples.


RESULTS
There was no TGFbetas, TbetaRII mRNA expression or telomerase activity in the control rat cholangiocytes. The expression of TGFbeta1, TbetaRII was increased in regenerative, hyperplastic, dysplastic cholangiocytes and cholangiocarcinoma (CC) cells. The expression of TGFbeta2 mRNA was observed in only a part of hyperplastic, dysplastic cholangiocytes. TGFbeta3 expression was very weak, only in hyperplastic lesion. There was positive telomerase activity in the regenerative, hyperplastic, dysplastic cholangiocytes, and CC cells. Stroma fibroblasts of these lesions also showed positive TGFbetas, TbetaRII mRNA expression and telomerase activity.


CONCLUSIONS
There were TGFbetas, TbetaRII expression and telomerase activity in hyperplastic, dysplastic cholangiocytes, cholangiocarcinoma cells as well as in stroma fibroblasts during cholangiocarcinogenesis. Their expression or activity is important in cholangiocarcinogenesis andstroma formation.
Lu
Jian-Ping
JP
Department of Pathology, Medical Center of Fudan University (Former Shanghai Medical University), Shanghai 200032, China. lu_jp@hotmail.com
Mao
Jian-Qun
JQ
Li
Ming-Sheng
MS
Lu
Shi-Lun
SL
Hu
Xi-Qi
XQ
Zhu
Shi-Neng
SN
Nomura
Shintaro
S
D000818:Animals; D001391:Azo Compounds; D001555:Benzene Derivatives; D018281:Cholangiocarcinoma / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics*; D019098:Telomerase / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000235:genetics*
C473128:3'-methyl 4-dimethylazobenzene; D001391:Azo Compounds; D001555:Benzene Derivatives; D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II; D019098:Telomerase