title: Capillarization of the sinusoids in liver fibrosis: noninvasive assessment with contrast-enhanced MRI in the rabbit.
abstract: Sinusoidal capillarization induces microcirculatory changes in liver cirrhosis and fibrosis. The purpose of this study was to assess whether contrast-enhanced MRI can be used to demonstrate the effects of sinusoidal capillarization in liver fibrosis. Dynamic MRI after injection of a low-molecular-weight contrast agent of 0.56 kDa (Gd-DOTA), and two high-molecular-weight contrast agents of 6.47 kDa and 52 kDa (P792 and P717) was performed in rabbits with liver fibrosis induced by cholesterol and diethylstilbestrol. The hepatic distribution volume accessible to the high-molecular-weight agents decreased in the rabbits with liver fibrosis (P792: 7.8% +/- 1.7% vs. 10.1% +/- 1.8% in normal rabbits, P =.038; P717: 6.2% +/- 2.1% vs. 9.7% +/- 1.6% in normal rabbits, P =.007), whereas the hepatic mean transit time (MTT) of the low-molecular-weight agent was increased (15.9 +/- 8.0 s vs. 8.8 +/- 2.6 s in normal rabbits, P =.015). In rabbits with liver fibrosis, the clearance of indocyanine green (ICG) was correlated with the volume accessible to the high-molecular-weight agents (P792: r = 0.810, P =.015; P717: r = 0.857, P =.007). The collagen content of the liver was inversely correlated with the distribution volume of P717 (r = -.833, P =.010) and with the ICG clearance (r = -.810, P =.015). It was concluded that the microcirculatory changes induced by sinusoidal capillarization in liver fibrosis can be demonstrated noninvasively with MRI.
authors.affiliation: Department of Radiology, Université Catholique de Louvain, St-Luc University Hospital, Avenue Hippocrate 10, B-1200 Brussels, Belgium. vanbeers@rdgn.ucl.ac.be
authors.forename: Bernard E
authors.initials: BE
authors.lastname: Van Beers
authors.forename: Roland
authors.initials: R
authors.lastname: Materne
authors.forename: Laurence
authors.initials: L
authors.lastname: Annet
authors.forename: Laurent
authors.initials: L
authors.lastname: Hermoye
authors.forename: Christine
authors.initials: C
authors.lastname: Sempoux
authors.forename: Frank
authors.initials: F
authors.lastname: Peeters
authors.forename: Anne M
authors.initials: AM
authors.lastname: Smith
authors.forename: Jacques
authors.initials: J
authors.lastname: Jamart
authors.forename: Yves
authors.initials: Y
authors.lastname: Horsmans
mesh_terms: D000818:Animals; D002199:Capillary Permeability; D002784:Cholesterol; D003287:Contrast Media; D006571:Heterocyclic Compounds; D007091:Image Processing, Computer-Assisted; D008099:Liver / Q000098:blood supply*; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000175:diagnosis* / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D008297:Male; D008833:Microcirculation; D009942:Organometallic Compounds; D011817:Rabbits
chemical_list: D003287:Contrast Media; D006571:Heterocyclic Compounds; D009942:Organometallic Compounds; D002784:Cholesterol; C050823:gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate