title: Gelatinase A (MMP-2), collagenase-2 (MMP-8), and laminin-5 gamma2-chain expression in murine inflammatory bowel disease (ulcerative colitis).
abstract: Dextran sulfate sodium-induced inflammatory bowel disease in mice resembles human ulcerative colitis. In inflammatory bowel diseases matrix metalloproteinases contribute to tissue degradation. Laminin-5 is an anchoring filament protein in the basement membrane area that can be cleaved by matrix metalloproteinases. We investigated the expression of matrix metalloproteinases-2 and -8 and laminin-5 gamma2-chain in dextran sulfate sodium-induced mice by immunohistochemistry and in situ hybridization. Matrix metalloproteinase-8 expression was evidenced in the colon surface epithelial cells and the protein was more abundant in dextran sulfate sodium-induced mice colon. Matrix metallproteinase-2 and laminin-5 gamma2-chain colocalized in the colon surface epithelial cells and in the basement membrane zone as demonstrated by double immunostaining. In dextran sulfate sodium-induced colon, matrix metalloproteinase-2 immunoreactivity was detected in epithelial cells in the lower parts of the crypt and surrounding the degraded crypts. Matrix metalloproteinase-2 and -8 could participate in the local epithelial inflammatory processes and tissue destruction. The presence of laminin-5 gamma2-chain indicates alternative anchoring mechanisms in the colon, a compartment devoid of hemidesmosomes.
authors.affiliation: Department of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Biomedicum Helsinki, Finland.
authors.forename: Emma
authors.initials: E
authors.lastname: Pirilä
authors.forename: Nungavarm S
authors.initials: NS
authors.lastname: Ramamurthy
authors.forename: Timo
authors.initials: T
authors.lastname: Sorsa
authors.forename: Tuula
authors.initials: T
authors.lastname: Salo
authors.forename: Jarkko
authors.initials: J
authors.lastname: Hietanen
authors.forename: Päivi
authors.initials: P
authors.lastname: Maisi
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000378:metabolism*; D003106:Colon / Q000378:metabolism*; D016264:Dextran Sulfate; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020784:Matrix Metalloproteinase 8 / Q000378:metabolism*; D051379:Mice; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D016264:Dextran Sulfate; D020778:Matrix Metalloproteinase 2; D020784:Matrix Metalloproteinase 8; D000097794:Kalinin