Caveolin-3 expression during early chicken development.
Caveolin-3, a protein that is correlated with caveolae, is found in muscle cells, especially during their differentiation. Although the distribution of caveolin-3 has been studied in cases such as adult and late embryonic mammalians, the expression of caveolin-3 has not been clearly defined during chicken development. In this study, we detected intense caveolin-3 immunoreactivity (IR) as early as embryonic day 4 (E4), most of the signals were localized within the neural tube and myotome. While IRs in the brain occurred in radial glia at E6, these intensities were reduced to an almost undetectable level at E8. In the case of muscle cells, the exclusive localization of caveolin-3 in the cytoplasmic membrane was detected even at E11, much earlier than in mammalian muscle tissues. Although the caveolin-3 IR pattern was similar to that reported by previous studies, we found some interesting mismatches in the case of avian tissues. Although we are unable to explain caveolin-3 expression patterns in the early embryonic stages, this study could provide a basis for further study on the function of caveolin-3 in avian embryogenesis.
Shin
Dong Hoon
DH
Department of Anatomy, Seoul National University, Seoul, South Korea.
Kim
Jin-Soo
JS
Kwon
Bum Sun
BS
Lee
Kun-Sei
KS
Kim
Jong Wan
JW
Kim
Myung Ho
MH
Cho
Sa Sun
SS
Lee
Wang Jae
WJ
D000818:Animals; D019521:Body Patterning / Q000502:physiology; D021941:Caveolae / Q000378:metabolism* / Q000648:ultrastructure; D051244:Caveolin 3; D022461:Caveolins / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D002490:Central Nervous System / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D002642:Chick Embryo / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D007150:Immunohistochemistry; D018482:Muscle, Skeletal / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism / Q000648:ultrastructure; D019170:Somites / Q000166:cytology / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism* / Q000648:ultrastructure
D051244:Caveolin 3; D022461:Caveolins