Intestinal epithelial CD23 mediates enhanced antigen transport in allergy: evidence for novel splice forms.
We previously demonstrated enhanced transepithelial antigen transport in the intestine of allergic rodents associated with elevated expression of the low-affinity IgE receptor CD23 on enterocytes. Here, we examined the role of CD23 in the transport phenomenon using CD23-/- mice and characterized the isoform of intestinal epithelial CD23. Jejunal segments of sensitized mice were challenged with antigen. Enhanced transepithelial antigen transport and transmucosal antigen flux were found in the intestine of sensitized CD23+/+ but not CD23-/- mice. RT-PCR showed that enterocytes expressed only the isoform b of CD23. Sequencing revealed classic and alternative CD23b transcripts lacking exon 5 (bDelta5) or 6, all of which were translated into functional IgE receptors. The protein encoded by bDelta5 but not the classic b transcript was able to mediate the uptake of anti-CD23 or IgE, whereas both CD23 proteins were internalized after binding to IgE/antigen complexes. Our results suggest that the classic and alternative forms of CD23b display distinct endocytic properties, suggesting that they are likely to play different roles in transepithelial transport of IgE and allergens.
Yu
Linda C H
LC
Intestinal Disease Research Programme, HSC 3N5C, McMaster University, 1200 Main Street W., Hamilton, Ontario, Canada L8N 3Z5.
Montagnac
Guillaume
G
Yang
Ping-Chang
PC
Conrad
Daniel H
DH
Benmerah
Alexandre
A
Perdue
Mary H
MH
D000818:Animals; D000941:Antigens / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005091:Exons; D015870:Gene Expression / Q000276:immunology; D006967:Hypersensitivity / Q000378:metabolism* / Q000503:physiopathology*; D007073:Immunoglobulin E / Q000097:blood; D007408:Intestinal Absorption / Q000276:immunology; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D007536:Isomerism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis; D017455:Receptors, IgE / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D000941:Antigens; D012333:RNA, Messenger; D017455:Receptors, IgE; D007073:Immunoglobulin E