title: Effects of alkali and simulated gastric and intestinal fluids on danazol stability.
abstract: The degradation kinetics of methanolic solution of danazol (0.020% w/v) in aqueous buffers and sodium hydroxide was investigated using stability-indicating HPLC method. The drug degrades in alkaline medium through a base-catalysed proton abstraction rather than via an oxidative mechanism involving oxygen species. The degradation followed pseudo-first-order kinetics. The rates pH-profile exhibited specific base catalysis. The stability of the drug was found to be dependent on pH, buffer concentration, buffer species (acetate, borate, phosphate) and temperature. The ionic strength did not affect the stability of the drug. The energy of activation according to Arrhenius plot was estimated to be 22.62 kcal mol(-1) at pH 12 and temperatures between 30 and 60 degrees C. The effect of simulated gastric and intestinal fluids on the drug stability was also investigated. Two major hydrolytic degradation products were separated and identified by IR, NMR and mass spectrometry and the degradative pathway suggested.
authors.affiliation: Department of Pharmaceutical Chemistry, College of Pharmacy, P.O. Box 2457, King Saud University, 11451, Riyadh, Saudi Arabia.
authors.forename: E A
authors.initials: EA
authors.lastname: Gadkariem
authors.forename: H A
authors.initials: HA
authors.lastname: El-Obeid
authors.forename: M A
authors.initials: MA
authors.lastname: Abounassif
authors.forename: S M
authors.initials: SM
authors.lastname: Ahmed
authors.forename: K E E
authors.initials: KE
authors.lastname: Ibrahim
mesh_terms: D000468:Alkalies / Q000378:metabolism; D002021:Buffers; D003613:Danazol / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D004355:Drug Stability; D005750:Gastric Juice / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007419:Intestinal Secretions / Q000378:metabolism*
chemical_list: D000468:Alkalies; D002021:Buffers; D003613:Danazol