title: Singlet oxygen quenching by dietary carotenoids in a model membrane environment.
abstract: The ability of several dietary carotenoids to quench singlet oxygen in a model membrane system (unilamellar DPPC liposomes) has been investigated. Singlet oxygen was generated in both the aqueous and the lipid phase, with quenching by a particular carotenoid independent of the site of generation. However, singlet oxygen quenching is dependent on the carotenoid incorporated; xanthophylls exhibit a marked reduction in efficiency compared to the hydrocarbon carotenoids. Lycopene and beta-carotene exhibit the fastest singlet oxygen quenching rate constants (2.3-2.5 x 10(9)M(-1)s(-1)) with lutein the least efficient (1.1 x 10(8)M(-1)s(-1)). The other carotenoids, astaxanthin and canthaxanthin, are intermediate. Zeaxanthin exhibits anomalous behavior, and singlet oxygen quenching decreases with increasing amounts of zeaxanthin, leading to nonlinear plots for the decay of singlet oxygen with zeaxanthin concentration. Such differences are discussed in terms of carotenoid structure and their influence on the properties of the lipid membrane. The formation of aggregates by the polar carotenoids is also proposed to be of significance in their ability to quench singlet oxygen.
authors.affiliation: School of Chemistry and Physics, Lennard-Jones Laboratories, Keele University, Staffordshire, ST5 5BG, UK.
authors.forename: Ann
authors.initials: A
authors.lastname: Cantrell
authors.forename: D J
authors.initials: DJ
authors.lastname: McGarvey
authors.forename: T George
authors.initials: TG
authors.lastname: Truscott
authors.forename: Fiorenza
authors.initials: F
authors.lastname: Rancan
authors.forename: Fritz
authors.initials: F
authors.lastname: Böhm
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000975:Antioxidants / Q000494:pharmacology; D002338:Carotenoids / Q000737:chemistry / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects*; D017666:Deuterium Oxide / Q000737:chemistry; D007700:Kinetics; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D014975:Lutein / Q000494:pharmacology; D000077276:Lycopene; D008567:Membranes, Artificial; D008956:Models, Chemical; D010100:Oxygen / Q000378:metabolism*; D013997:Time Factors; D024341:Xanthophylls / Q000737:chemistry; D065146:Zeaxanthins; D019207:beta Carotene / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D008055:Lipids; D008081:Liposomes; D008567:Membranes, Artificial; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene; D015060:1,2-Dipalmitoylphosphatidylcholine; D002338:Carotenoids; D017666:Deuterium Oxide; D010100:Oxygen; D000077276:Lycopene; D014975:Lutein