MRP1-transfected cells do not show increased resistance against oxidative stress.
Sensitivity of V79 Chinese hamster cells and V79 cells transfected with human MRP1 gene to several agents inducing oxidative stress was compared. Cells overexpressing MRP1 did not show increased resistance to tert-butyl hydroperoxide, diamide, paraquat, menadione, dichromate and carmustine as estimated by cell survival and DNA damage assessed by comet assay. These findings suggest that overexpression of MRP1 does not confer increased resistance to oxidative stress.
Balcerczyk
Aneta
A
Department of Molecular Biophysics, University of Lódź, 90-237 Lódź, Poland. kbm@biol.uni.lodz.pl
Rychlik
Błazej
B
Kruszewski
Marcin
M
Burchell
Brian
B
Bartosz
Grzegorz
G
D000818:Animals; D001692:Biological Transport; D015153:Blotting, Western; D001643:Bridged Bicyclo Compounds / Q000378:metabolism; D016466:CHO Cells; D002330:Carmustine / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival; D020552:Comet Assay; D006224:Cricetinae; D004249:DNA Damage; D003958:Diamide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics* / Q000502:physiology*; D018384:Oxidative Stress; D010269:Paraquat / Q000494:pharmacology; D011192:Potassium Dichromate / Q000494:pharmacology; D014162:Transfection; D024483:Vitamin K 3 / Q000494:pharmacology; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D001643:Bridged Bicyclo Compounds; D002330:Carmustine; D003958:Diamide; D005978:Glutathione; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D010269:Paraquat; D011192:Potassium Dichromate; D024483:Vitamin K 3; D020122:tert-Butylhydroperoxide; C080120:glutathione-bimane; C421109:multidrug resistance-associated protein 1