[Effect of neuroprotectant agent combined with cocktail on expression of anti-apoptotic protein bcl-2 in rats after focal cerebral ischemia].
OBJECTIVE
To investigate whether the protective effect of therapy with different combined neuroprotectant agents was better than that of single agent on focal cerebral ischemia.


METHODS
The right middle cerebral artery in the rats was occluded with suture occlusion technique. The rats were divided into five groups treated with FDP (50 mg/kg, n = 10), MK-801 (1 mg/kg, n = 10) and NAC (150 mg/kg, n = 10) singly, or in combination, respectively, by intraperitoneal infusion 30 minutes after vessel occlusion. The rats were weighed and assessed neurologically, based on a 5-point scale, six and 24 hours after focal cerebral ischemia. The expression of anti-apoptotic protein bcl-2 was observed with SDS-PAGE protein electrophoresis and Western blot technique.


RESULTS
The optical density of bcl-2 increased more distinctly in the rats treated with combined neuroprotective agents than that with any single agent six and 24 hours after cerebral ischemia, with a statistically significant difference (P < 0.05).


CONCLUSIONS
Treatment with combined neuroprotectant agents could un-regulate the anti-apoptotic protein bcl-2 more distinctly than that with any single agents. Combined use of neuroprotectants might be more effective than that of single agent in protecting rats' brain from ischemia.
Pan
Dianqing
D
Department of Neurology, Harrison International Peace Hospital, Hebei Medical University, Hengshui 053000, China.
Li
Zhimei
Z
D000111:Acetylcysteine / Q000008:administration & dosage; D000199:Actins / Q000032:analysis; D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism; D016291:Dizocilpine Maleate / Q000008:administration & dosage; D004359:Drug Therapy, Combination; D005635:Fructosediphosphates / Q000008:administration & dosage; D008297:Male; D008970:Molecular Weight; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar
D000199:Actins; D005635:Fructosediphosphates; D018696:Neuroprotective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D016291:Dizocilpine Maleate; C029063:fructose-1,6-diphosphate; D000111:Acetylcysteine