Detection of HBV, PCNA and GST-pi in hepatocellular carcinoma and chronic liver diseases.
OBJECTIVE
To investigate the change of HBV DNA, PCNA and GST-pi in chronic liver disease and hepatocellular carcinoma (HCC).


METHODS
Hepatitis B surface antigen (HBsAg), proliferating cell nuclear antigen (PCNA) and glutathione S-transferases (GST-pi) were detected by immunohistochemical staining and HBV DNA was detected by in situ hybridization (ISH) in formalin-fixed and paraffin-embedded sections with a total of 111 specimens of chronic hepatitis, liver cirrhosis, paratumorous tissue, HCC and normal liver tissue.


RESULTS
The positive rates of HBsAg and HBVDNA were 62.5 %(15/24) and 75.0 %(12/16) in chronic hepatitis, 64.0 %(16/25) and 83.3 %(15/18) in liver cirrhosis, 72.7 %(16/22) and 85.7 %(12/14) in the paratumorous tissu and 45.0 %(14/31) and 64.3 %(9/14) in HCC. The positive HBVDNA granules in chronic hepatitis, liver cirrhosis and the paratumorous tissue were more intense than that in HCC. The positive rates of PCNA and GST-pi were 34.8 %(8/23) and 25.0 %(4/16) in chronic hepatitis, 73.7 %(14/19) and 17.6 %(3/17) in liver cirrhosis, 86.7 %(13/15) and 53.3 %(8/15) in the paratumorous tissue, 100 %(15/15) and 60.0 %(9/15) in HCC, respectively, and the positive rate of GST-pi in the paratumorous tissue was significantly higher than that in the liver cirrhosis without tumor (P<0.05), but same as that in HCC (P>0.05).


CONCLUSIONS
The HBV infection may increase expression of PCNA and GST-pi. The paratumor cirrhosis may be a sequential lesion of precancerous cirrhosis around HCC.
Shen
Li-Juan
LJ
Department of Pathology, Kunming Medical College, Kunmming 650031, Yunnan Province, China. wycslj@public.km.yn.cn
Zhang
Hua-Xian
HX
Zhang
Zong-Ji
ZJ
Li
Jin-Yun
JY
Chen
Ming-Qin
MQ
Yang
Wei-Bo
WB
Huang
Run
R
D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000821:virology*; D002908:Chronic Disease; D005982:Glutathione Transferase / Q000378:metabolism*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D008107:Liver Diseases / Q000378:metabolism / Q000821:virology*; D008113:Liver Neoplasms / Q000378:metabolism / Q000821:virology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*
D018809:Proliferating Cell Nuclear Antigen; D005982:Glutathione Transferase