title: Accumulation of phosphorylated repressor for gibberellin signaling in an F-box mutant.
abstract: Gibberellin (GA) regulates growth and development in plants. We isolated and characterized a rice GA-insensitive dwarf mutant, gid2. The GID2 gene encodes a putative F-box protein, which interacted with the rice Skp1 homolog in a yeast two-hybrid assay. In gid2, a repressor for GA signaling, SLR1, was highly accumulated in a phosphorylated form and GA increased its concentration, whereas SLR1 was rapidly degraded by GA through ubiquitination in the wild type. We conclude that GID2 is a positive regulator of GA signaling and that regulated degradation of SLR1 is initiated through GA-dependent phosphorylation and finalized by an SCF(GID2)-proteasome pathway.
authors.affiliation: BioScience Center, Nagoya University, Nagoya 464-8601, Japan.
authors.forename: Akie
authors.initials: A
authors.lastname: Sasaki
authors.forename: Hironori
authors.initials: H
authors.lastname: Itoh
authors.forename: Kenji
authors.initials: K
authors.lastname: Gomi
authors.forename: Miyako
authors.initials: M
authors.lastname: Ueguchi-Tanaka
authors.forename: Kanako
authors.initials: K
authors.lastname: Ishiyama
authors.forename: Masatomo
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Jeong
authors.forename: Gynheung
authors.initials: G
authors.lastname: An
authors.forename: Hidemi
authors.initials: H
authors.lastname: Kitano
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Ashikari
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsuoka
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D018388:Codon, Terminator; D004790:Enzyme Induction; D017343:Genes, Plant; D005875:Gibberellins / Q000378:metabolism* / Q000494:pharmacology; D008025:Ligases / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism; D010641:Phenotype; D010766:Phosphorylation; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D017434:Protein Structure, Tertiary; D012639:Seeds / Q000378:metabolism; D015398:Signal Transduction; D025801:Ubiquitin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases; D000516:alpha-Amylases / Q000096:biosynthesis
chemical_list: D018388:Codon, Terminator; D005875:Gibberellins; D010940:Plant Proteins; D025801:Ubiquitin; C007842:gibberellic acid; D044767:Ubiquitin-Protein Ligases; D000516:alpha-Amylases; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease; D008025:Ligases