Role of dietary fat type in the development of adiposity from dietary obesity-susceptible Sprague-Dawley rats.
The present study was designed to define how dietary fat type regulates body adiposity in dietary obesity-susceptible (DOS) Sprague-Dawley (SD) rats. Eighty-three SD rats received a purified diet containing 50 g maize oil (MO)/kg for 3 weeks and then thirty-nine of the rats, designated as the DOS rats, were allotted to diets containing 160 g MO (DOS-MO), beef tallow (DOS-BT) or fish oil (DOS-FO)/kg for 9 weeks. As a result of the experiment, the DOS-FO rats had significantly (P<0.05) reduced weight gain and abdominal and epididymal fat-pad mass than the DOS-MO and DOS-BT rats. Serum leptin level was also significantly (P<0.05) lower in the DOS-FO rats; however, hypothalamic leptin receptor (a and b) mRNA and neuropeptide Y expressions were not altered by dietary fat sources. A lower acetyl-CoA carboxylase mRNA expression in the liver was observed in the DOS-FO group, whereas hepatic peroxisome proliferator-activated receptor-gamma mRNA and protein expressions were markedly elevated in the DOS-FO group compared with those in the other groups. We did not observe differences in acetyl-CoA carboxylase and peroxisome proliferator-activated receptor-gamma expressions in epididymal fat of the DOS rats consuming MO, BT or FO. It is concluded from our present observations that dietary fat type, especially that rich in FO, plays a potential role in down-regulation of adiposity by altering hepatic lipogenic genes, rather than feeding behaviour, in the DOS-SD rats.
Jang
In Surk
IS
Department of Animal Science & Biotechnology, RAIRC, Jinju National University, Chilam-Dong 150, Jinju, KyeongNam, Korea. isjang@jinju.ac.kr
Hwang
Dae Yeon
DY
Chae
Kab Ryong
KR
Lee
Ju Eun
JE
Kim
Yong Kyu
YK
Kang
Tae Seok
TS
Hwang
Jin Hee
JH
Lim
Chae Hyung
CH
Huh
Young Bum
YB
Cho
Jung Sik
JS
D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects*; D000818:Animals; D002417:Cattle; D003314:Corn Oil / Q000494:pharmacology; D004041:Dietary Fats / Q000494:pharmacology*; D004198:Disease Susceptibility; D004435:Eating; D005395:Fish Oils / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D020738:Leptin / Q000097:blood; D008099:Liver / Q000378:metabolism; D008297:Male; D009478:Neuropeptide Y / Q000378:metabolism; D009765:Obesity / Q000209:etiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D054411:Receptors, Leptin; D014157:Transcription Factors / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects
D004041:Dietary Fats; D005395:Fish Oils; D020738:Leptin; D009478:Neuropeptide Y; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D054411:Receptors, Leptin; D014157:Transcription Factors; D003314:Corn Oil; D000103:Acetyl-CoA Carboxylase