Localization of classical swine fever virus from chronically infected pigs by in situ hybridization and immunohistochemistry.
Classical swine fever (CSF) virus (CSFV) nucleic acid and antigen were detected in 15 pigs with naturally occurring chronic CSF by in situ hybridization and immunohistochemistry. The most consistent and prominent microscopic lesions were perivascular mononuclear cell infiltration and gliosis in the central nervous system of pigs with chronic CSF. Positive cells typically exhibited a dark brown (in situ hybridization) or red (immunohistochemistry) reaction product in the cytoplasm without background staining. A positive signal for both in situ hybridization and immunohistochemistry was detected in mononuclear cells and lymphocytes of lymphoid tissues. Viral nucleic acid was detected in some tissue sections in the absence of viral antigen. The in situ hybridization technique developed in this study was useful for the detection of CSFV RNA in tissues taken from chronically infected pigs and may be a valuable technique for studying the pathogenesis of chronic CSFV infection.
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Chae
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D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D001921:Brain / Q000473:pathology / Q000821:virology; D006691:Classical Swine Fever / Q000175:diagnosis / Q000473:pathology / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary; D017403:In Situ Hybridization / Q000379:methods / Q000662:veterinary; D008221:Lymphoid Tissue / Q000473:pathology / Q000821:virology; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D013552:Swine
D000956:Antigens, Viral; D012367:RNA, Viral