Treatment of human immunodeficiency virus type 1 virions depleted of cyclophilin A by natural endogenous reverse transcription restores infectivity.
We have previously shown that virions with nef deleted can be restored to wild-type infectivity by treatment to induce natural endogenous reverse transcription (NERT). Since Nef and cyclophilin A (CyPA) appear to act in similar ways on postentry events, we determined whether NERT treatment would restore infectivity to virions depleted of CyPA. Our results show that the infectivity of virions depleted of CyPA by treatment with cyclosporine A could be restored by NERT treatment, while mutants in the CyPA binding loop of capsid could only be partially restored. These results suggest that CyPA is involved in some aspect of the uncoating process.
Khan
Mahfuz
M
Department of Microbiology, Biochemistry, and Immunology, Morehouse School of Medicine, Atlanta, Georgia 30310, USA.
Garcia-Barrio
Minerva
M
Powell
Michael D
MD
D036022:Capsid Proteins / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D021984:Cyclophilin A / Q000172:deficiency / Q000502:physiology*; D016572:Cyclosporine / Q000494:pharmacology; D005814:Genes, Viral; D015754:Genes, nef; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology; D009154:Mutation; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics / Q000502:physiology
D036022:Capsid Proteins; C028391:G protein, vesicular stomatitis virus; D007166:Immunosuppressive Agents; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D016572:Cyclosporine; D021984:Cyclophilin A