title: Proteomic analysis of rat kidney cortex following treatment with gentamicin.
abstract: The regionally specific structure and function of the kidney renders it susceptible to toxic exposure. To characterize these changes at the proteome level, we have investigated the effects on protein expression following treatment with gentamicin. The more than 20 proteins identified were involved in the citric acid cycle, gluconeogenesis, fatty acid synthesis, and transport or cellular stress responses. These results strongly support the notion that energy production is impaired and mitochondrial dysfunction is involved in gentamicin-induced nephrotoxicity.
authors.affiliation: GlaxoSmithKline, New Frontiers Science Park, Third Avenue, Harlow, Essex CM19 5AW, UK.
authors.forename: Joanne
authors.initials: J
authors.lastname: Charlwood
authors.forename: J Mark
authors.initials: JM
authors.lastname: Skehel
authors.forename: Nick
authors.initials: N
authors.lastname: King
authors.forename: Patrick
authors.initials: P
authors.lastname: Camilleri
authors.forename: Peter
authors.initials: P
authors.lastname: Lord
authors.forename: Peter
authors.initials: P
authors.lastname: Bugelski
authors.forename: Uzma
authors.initials: U
authors.lastname: Atif
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000633:toxicity; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D005839:Gentamicins / Q000494:pharmacology* / Q000633:toxicity; D007672:Kidney Cortex / Q000166:cytology / Q000187:drug effects* / Q000473:pathology / Q000502:physiology*; D008297:Male; D013058:Mass Spectrometry; D008928:Mitochondria / Q000378:metabolism; D008954:Models, Biological; D020543:Proteome / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D020543:Proteome