Synthesis and DNA binding properties of a cationic 2,2':6',2"-terpyridine cobalt(II) complex containing an oligopeptide.
The first example of DNA metallointercalator containing an oligopeptide moiety is presented. The ternary cobalt(II) complex [(DOTA)Co(II)(TPY)](ClO(4))(2) (DOTA=1,4,7,10-tetraaza-cyclododecane-2,9-dione, TPY=2, 2':6',2"-terpyridine) was found to be efficient in binding ct-DNA. The binding constant was determined by spectrophotometric titration. In vitro antitumor studies shows that this complex has significant antitumor activity. The single-crystal of a six coordinated oligopeptide cobalt(II) complex [Co(II)(DOTA)(2)](ClO(4))(2) is also reported. The stabilities and species distributions of Co(II)-DOTA (1:1) and DOTA-Co(II)-TPY (1:1:1) systems were investigated by potentiometry titration.
Gao
Jian
J
Department of Chemistry, Texas A&M University, College Station, TX 77842-3012, USA.
Reibenspies
Joseph H
JH
Martell
Arthur E
AE
D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004354:Drug Screening Assays, Antitumor; D004355:Drug Stability; D006801:Humans; D007364:Intercalating Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D007941:Leukemia P388 / Q000188:drug therapy; D046911:Macromolecular Substances; D051379:Mice; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D013053:Spectrophotometry; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D007364:Intercalating Agents; D046911:Macromolecular Substances; D009842:Oligopeptides; D009942:Organometallic Compounds; C445104:bis(terpyridine)cobalt(II) chloride; D004247:DNA