Differential regulation of GABA B receptor subunit expression and function.
The GABA(B) receptor is a G protein-coupled heterodimer composed of GABA(B1) and GABA(B2) subunits. In the present study, experiments were undertaken to examine the relationship between GABA(B) receptor function and subunit expression in the rat lumbar spinal cord following pharmacological and physiological manipulation of this receptor system. Although formalin-induced hind paw inflammation increases the production of GABA(B1) and GABA(B2) protein in the spinal cord within 24 h, there is no change in receptor function, as measured by the baclofen-stimulated guanosine 5'-O-(3-[(35)S]thiotriphosphate) ([(35)S]GTPgammaS) binding assay. Conversely, although chronic (7 days) administration of baclofen, a GABA(B) receptor agonist, abolishes baclofen-stimulated [(35)S]GTPgammaS binding in the spinal cord tissue, causes tolerance to the sedative and antinociceptive effects of the drug, increases the number of formalin-induced hind paw flinches, and induces mechanical hyperalgesia, this treatment had no effect on the levels of GABA(B1) or GABA(B2) mRNAs in the lumbar spinal cord. The results indicate a lack of concordance between expression of GABA(B1) and GABA(B2) subunits and GABA(B) receptor function, suggesting these subunit proteins may serve multiple functions in the cells. Moreover, these findings indicate that nongenomic mechanisms are primarily responsible for the GABA(B) receptor desensitization that occurs during prolonged exposure to receptor agonist.
Sands
S A
SA
Department of Pharmacology, Toxicology and Therapeutics, Kansas University School of Medicine, Kansas City, Kansas 66160-7424, USA.
McCarson
K E
KE
Enna
S J
SJ
D000818:Animals; D001418:Baclofen / Q000494:pharmacology; D001665:Binding Sites; D005557:Formaldehyde / Q000494:pharmacology; D018755:GABA Agonists / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D008297:Male; D010147:Pain Measurement / Q000187:drug effects; D021122:Protein Subunits / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D018080:Receptors, GABA-B / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D013462:Sulfur Radioisotopes
D018755:GABA Agonists; D021122:Protein Subunits; D012333:RNA, Messenger; D018080:Receptors, GABA-B; D013462:Sulfur Radioisotopes; D005557:Formaldehyde; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D001418:Baclofen