Functional EF-Tu with large C-terminal extensions in an E coli strain with a precise deletion of both chromosomal tuf genes.
An Escherichia coli strain was constructed in which both chromosomal genes encoding elongation factor (EF)-Tu (tufA and tufB) have been inactivated with precise coding sequence replacements. A tufA gene in an expression vector is supplied as the sole EF-Tu source. By using plasmid replacement, based on plasmid incompatibility, mutant EF-Tu variants with a large C'-terminal extension up to 270 amino acids were studied and proved to be functional in a strain lacking the chromosomal tufA and tufB genes.
Schnell
Robert
R
Department of Microbiology, Stockholm University, S-106 91 Stockholm, Sweden.
Abdulkarim
Farhad
F
Kálmán
Miklós
M
Isaksson
Leif A
LA
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D017353:Gene Deletion; D010444:Peptide Elongation Factor Tu / Q000235:genetics*; D010957:Plasmids
D001426:Bacterial Proteins; D017931:DNA Primers; D010444:Peptide Elongation Factor Tu