title: Immunohistochemical localization of S100-like protein in non-mammalian kidney.
abstract: The immunolocalization of S100-like protein was investigated in the kidney of saltwater fishes (Dicentrarchus labrax; Coris julis; Serranus cabrilla; Scorpaena porcus), amphibia (Rana aesculenta), reptiles (Lacerta viridis), and aves (Gallus domesticus; Strutio camelus). S100-like immunoreactivity was detected in the juxtaglomerular cells of all saltwater fishes studied. No immunoreactivity was observed in other tracts of the nephron or in the interstitial tissue. In frog kidney, S100-like immunoreactive cells were localized in the proximal tubule, singly distributed or placed side by side in clusters of two or three cells. S100-like immunoreactive cells were distributed in the distal and in the collecting tubules in lizard, chicken, and ostrich kidney. In the distal tubule of lizard kidney, S100-like immunoreactive cells were numerous and uniformly distributed. In lizard collecting tubules, S100-like immunoreactive cells showed less intense immunoreactivity than in the distal tubule, except for a cluster of cells at the junction with the initial collecting duct. In chicken and ostrich kidney, S100-like immunoreactive cells of the distal tubules were closely packed together. In the collecting tubules, S100-like immunoreactive cells were alternate to negative cells. These results indicate the high conservation degree of S100 proteins through phylogenesis and suggest a functional role for these proteins in the vertebrate kidney.
authors.affiliation: Dipartimento di Strutture, Funzioni e Tecnologie Biologiche, Università di Napoli "Federico II," 1 I-80137 Napoli, Italy. degirola@unina.it
authors.forename: Paolo
authors.initials: P
authors.lastname: De Girolamo
authors.forename: Nadia
authors.initials: N
authors.lastname: Arcamone
authors.forename: Gaetano Vincenzo
authors.initials: GV
authors.lastname: Pelagalli
authors.forename: Giuliana
authors.initials: G
authors.lastname: Gargiulo
mesh_terms: D000818:Animals; D002645:Chickens / Q000378:metabolism; D005399:Fishes / Q000378:metabolism; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism* / Q000648:ultrastructure; D007684:Kidney Tubules / Q000378:metabolism / Q000648:ultrastructure; D008116:Lizards / Q000378:metabolism; D008854:Microscopy, Electron; D011898:Ranidae / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism*; D012623:Seawater; D020310:Struthioniformes / Q000378:metabolism
chemical_list: D009418:S100 Proteins