Transfecting mammalian cells by DNA/calcium phosphate precipitates: effect of temperature and pH on precipitation.
Chowdhury
E H
EH
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
Sasagawa
Tadashi
T
Nagaoka
Masato
M
Kundu
A K
AK
Akaike
Toshihiro
T
D002118:Calcium / Q000494:pharmacology; D002130:Calcium Phosphates / Q000494:pharmacology*; D011232:Chemical Precipitation; D004247:DNA / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D014162:Transfection / Q000379:methods*
D002130:Calcium Phosphates; D004247:DNA; D008156:Luciferases; D002118:Calcium