Capillary electrophoresis of erythrocytes.
Capillary electrophoresis (CE) of erythrocytes from different sources under various conditions is reported in this paper. It was found that erythrocyte samples from sheep, duck, and human showed characteristic and reproducible elution peaks, and that the retention times of A-, B-, AB-, and O-type erythrocytes from human blood were distinctively different; even subtle differences, among individuals with the same blood type could be detected by CE. A strictly linear correlation was obtained between the peak area and the amount of human erythrocyte over a range of 4.8 x 10(2)-1.9 x 10(4) cells (r=0.999), indicating that CE could be used for rapid and accurate quantification of erythrocytes. Using this CE protocol, the decrease of the surface electrical charge of erythrocyte during storage was confirmed. Therefore, this work demonstrated that CE could be a useful alternative for characterizing and quantifying erythrocytes or other cells.
Lu
Wan-Hua
WH
Institute of Biotechnology, Fuzhou University, Gong-Ye-Ru Road 523, Fuzhou, Fujian Province 350002, People's Republic of China.
Deng
Wen-Han
WH
Liu
Shu-Tao
ST
Chen
Tian-Bao
TB
Rao
Ping-Fan
PF
D000017:ABO Blood-Group System / Q000097:blood; D000818:Animals; D004372:Ducks; D019075:Electrophoresis, Capillary / Q000379:methods*; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000166:cytology* / Q000502:physiology; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology; D012756:Sheep; D013997:Time Factors
D000017:ABO Blood-Group System