Expression of the acetylcholine release mechanism in various cells and reconstruction of the release mechanism in non-releasing cells.
After loading cells in culture with acetylcholine (ACh), it was possible to identify cells that express a calcium-dependent release mechanism and cells that do not release. Mediatophore transfection restored the release capability of non-releasing cells. The transfection of choline acetyltransferase and the vesicular ACh transporter (VAChT) in cells that have already mediatophore in their membrane enables to study the effect of VAChT on the release kinetics. We also studied the properties of the mediatophore "pore" as a function of the concentration of ACh and also its temporal properties. A reconstruction of the release mechanism in cells particularly graftable cells, appears now possibly for ACh and probably for other transmitters.
Malo
M
M
Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, 91198 Gif-sur-Yvette Cedex, France. michel.malo@creteil.inserm.fr
Israël
M
M
D000109:Acetylcholine / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000502:physiology; D020013:Calcium Signaling / Q000235:genetics / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004557:Electric Organ / Q000166:cytology / Q000378:metabolism; D004594:Electrophysiology; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005910:Glioma / Q000378:metabolism; D007700:Kinetics; D008564:Membrane Potentials / Q000502:physiology; D026901:Membrane Transport Proteins; D010957:Plasmids / Q000235:genetics; D014101:Torpedo; D014162:Transfection; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D014981:Xenopus
D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine; D002118:Calcium