title: Genomes to Life "Center for Molecular and Cellular Systems": a research program for identification and characterization of protein complexes.
abstract: Goal 1 of Department of Energy's Genomes to Life (GTL) program seeks to identify and characterize the complete set of protein complexes within a cell. Goal 1 forms the foundation necessary to accomplish the other objectives of the GTL program, which focus on gene regulatory networks and molecular level characterization of interactions in microbial communities. Together this information would allow cells and their components to be understood in sufficient detail to predict, test and understand the responses of a biological system to its environment. The Center for Molecular and Cellular Systems has been established to identify and characterize protein complexes using high through-put analytical technologies.A dynamic research program is being developed that supports the goals of the Center by focusing on the development new capabilities for sample preparation and complex separations, molecular level identification of the protein complexes by mass spectrometry, characterization of the complexes in living cells by imaging techniques, and bioinformatics and computational tools for the collection and interpretation of data and formation of databases and tools to allow the data to be shared by the biological community.
authors.affiliation: Oak Ridge National Laboratory, 1 Bethel Valley Road, Oak Ridge, TN 37831-6129, USA. buchananmv@ornl.gov
authors.forename: Michelle V
authors.initials: MV
authors.lastname: Buchanan
authors.forename: Frank W
authors.initials: FW
authors.lastname: Larimer
authors.forename: H Steven
authors.initials: HS
authors.lastname: Wiley
authors.forename: Steve J
authors.initials: SJ
authors.lastname: Kennel
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Squier
authors.forename: J Michael
authors.initials: JM
authors.lastname: Ramsey
authors.forename: Karin D
authors.initials: KD
authors.lastname: Rodland
authors.forename: Gregory B
authors.initials: GB
authors.lastname: Hurst
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: David
authors.initials: D
authors.lastname: Dixon
authors.forename: Mitchel J
authors.initials: MJ
authors.lastname: Doktycz
authors.forename: Steve
authors.initials: S
authors.lastname: Colson
authors.forename: Ray
authors.initials: R
authors.lastname: Gesteland
authors.forename: Carol
authors.initials: C
authors.lastname: Giometti
authors.forename: Malin
authors.initials: M
authors.lastname: Young
authors.forename: Michael
authors.initials: M
authors.lastname: Giddings
mesh_terms: D000818:Animals; D001695:Biology / Q000379:methods*; D016678:Genome; D006801:Humans; D013058:Mass Spectrometry; D011506:Proteins / Q000737:chemistry; D012106:Research
chemical_list: D011506:Proteins