Antisense knockdown of neuronal nitric oxide synthase antagonizes nitrous oxide-induced behavior.
The behavioral effects of nitrous oxide (N(2)O) were antagonized by non-specific inhibitors of nitric oxide synthase (NOS). To identify the isoform of NOS involved in this response, mice were pretreated with an antisense oligodeoxynucleotide (AS-ODN) against neuronal NOS, and then tested in a light/dark exploration paradigm. The AS-ODN but not the mismatch ODN significantly antagonized N(2)O induced behavior and also reduced NOS activity in the cerebellum and hippocampus. These results implicate neuronal NOS in the N(2)O response.
Li
Shuang
S
Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, 99164-6534, Pullman, WA, USA.
Dai
Yang
Y
Quock
Raymond M
RM
D000704:Analysis of Variance; D000818:Animals; D020137:Base Pair Mismatch; D001522:Behavior, Animal; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002940:Circadian Rhythm / Q000187:drug effects / Q000502:physiology; D004347:Drug Interactions; D005106:Exploratory Behavior / Q000187:drug effects*; D008297:Male; D051379:Mice; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000235:genetics; D052248:Nitric Oxide Synthase Type I; D009609:Nitrous Oxide / Q000494:pharmacology*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D013997:Time Factors
D020319:Oligodeoxyribonucleotides, Antisense; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496321:Nos1 protein, mouse; D009609:Nitrous Oxide