title: Improvements to EPA method 531.1 for the analysis of carbamates that resulted in the development of U.S. EPA Method 531.2.
abstract: This project is undertaken to fully optimize the U.S. Environmental Protection Agency Method 531.1 post-column chemistries and to incorporate recent advances in liquid chromatographic separation, post-column derivatization, and detection techniques. Sample preservation and storage stability studies establish citric acid as a suitable replacement for the caustic monochloroacetic acid in the current method and confirm its antimicrobial effectiveness. Performance of an alternate set of commercially available post-column reagents is also investigated. This research has resulted in the publication of Method 531.2, a high-performance liquid chromatographic direct injection method for the analysis of N-methylcarbamoyloximes and N-methylcarbamates using post-column derivatization and fluorescence detection.
authors.affiliation: Shaw Environmental, Inc., 26 W. Martin Luther King Drive, Cincinnati, OH 45268, USA. bassett.margie@EPA.gov
authors.forename: Martha V
authors.initials: MV
authors.lastname: Bassett
authors.forename: Steve C
authors.initials: SC
authors.lastname: Wendelken
authors.forename: Barry V
authors.initials: BV
authors.lastname: Pepich
authors.forename: David J
authors.initials: DJ
authors.lastname: Munch
mesh_terms: D002219:Carbamates; D002713:Chlorine / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000032:analysis*; D012015:Reference Standards; D013050:Spectrometry, Fluorescence; D014481:United States; D014484:United States Environmental Protection Agency
chemical_list: D002219:Carbamates; D007306:Insecticides; D002713:Chlorine