title: Gene silencing by adenovirus-delivered siRNA.
abstract: RNA interference is the process that double-stranded RNA induces the homology-dependent degradation of cognate mRNA mediated by 21-23 nucleotide short interfering RNA (siRNA). Here, we describe a simple virus vector for efficient delivery of siRNA into mammalian cells utilizing the well-defined H1-RNA promoter and conventional adenovirus. In this pilot study, p53 was targeted by this vector. Our results demonstrate efficient and specific knock-down of p53 in breast cancer MCF-7 and lung carcinoma A549 cells and indicate a prospective application of this siRNA expressing recombinant adenovirus system in functional genomics, cancer gene therapy and virus inhibition.
authors.affiliation: Department of Nuclear Medicine, University of Ulm, Robert-Koch-Strasse 8, D-89070 Ulm, Germany.
authors.forename: Changxian
authors.initials: C
authors.lastname: Shen
authors.forename: Andreas K
authors.initials: AK
authors.lastname: Buck
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Liu
authors.forename: Michael
authors.initials: M
authors.lastname: Winkler
authors.forename: Sven N
authors.initials: SN
authors.lastname: Reske
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D016158:Genes, p53; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D014407:Tumor Cells, Cultured