title: Macrophages at the skeletal tissue-device interface of loosened prosthetic devices express bone-related genes and their products.
abstract: Aseptic loosening of prosthetic arthroplasty is the most common reason for implant failure in adult orthopaedic reconstruction. At the interface of aseptic loosened prostheses, there is an abundance of particle-activated macrophages and other inflammatory cells. The role of these particle-laden macrophages in the osteogenic arm of the remodeling skeleton in this pathological condition is poorly understood. Molecular signaling by mesenchymal cells and mononuclear inflammatory cells residing in the interfacial tissues between bone and cement or prosthetic material of aseptically loosened joint prostheses was studied using in situ hybridization and immunohistochemical techniques. We found that a range of collagenous and noncollagenous matrix proteins, including osteopontin, osteocalcin, bone sialoprotein, and type I collagen, were produced in the periprosthetic tissue by foamy macrophages, as well as nearby osteogenic cells. The former accumulated in profusion in the three zones of interfacial tissues: pseudomembranous, fibrous, and osseous. Spindle mesenchymal cells in the fibrous zone failed to express any of the osteogenic mRNAs or proteins sought. The expression of bone-related genes and proteins by foamy macrophages at the interface of an aseptic loosened prosthesis may contribute to the disturbance of bone remodeling at this site.
authors.affiliation: School of Pathology, University of New South Wales, NSW, Sydney 2052, Australia. h.zreiqat@unsw.adv.au
authors.forename: H
authors.initials: H
authors.lastname: Zreiqat
authors.forename: R K
authors.initials: RK
authors.lastname: Kumar
authors.forename: B
authors.initials: B
authors.lastname: Markovic
authors.forename: B
authors.initials: B
authors.lastname: Zicat
authors.forename: C R
authors.initials: CR
authors.lastname: Howlett
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001391:Azo Compounds; D001672:Biocompatible Materials; D001842:Bone and Bones / Q000166:cytology*; D004396:Coloring Agents; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008264:Macrophages / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D010012:Osteogenesis / Q000235:genetics*; D019736:Prostheses and Implants; D014176:Protein Biosynthesis; D016707:Tissue Fixation
chemical_list: D001391:Azo Compounds; D001672:Biocompatible Materials; D004396:Coloring Agents; C011049:oil red O