New serum-free in vitro culture technique for midgestation mouse embryos.
Current in vitro culture methods for mouse embryos are critically dependent on specially prepared rodent serum. Rodent serum requires careful preparation and stringent assessment of serum quality, while commercially available whole embryo culture serum is expensive and shows considerable lot variability. Thus, preparation and testing of suitable serum represents a considerable investment of time and resources, particularly for laboratories with only short-term embryo culture requirements. In addition, serum supplementation of culture medium may introduce unknown serum components that could interfere with interpretation of experimental results, especially where the study is geared towards analysis of a specific growth factor. Here we describe the composition of a standardized serum free culture medium comprised of commercially available stem cell media supplements. With this method, we have successfully cultured midgestation stage mouse embryos and demonstrated, using both morphological and gene expression criteria, that these embryos exhibited proper developmental progression. We believe this method to be a significant advance in whole embryo culture technology that will be of particular use to laboratories needing to utilize whole embryo culture to study midgestation organogenesis.
Moore-Scott
Billie A
BA
Institute for Molecular Medicine and Genetics, Medical College of Georgia, Augusta Georgia 30606, USA.
Gordon
Julie
J
Blackburn
C Clare
CC
Condie
Brian G
BG
Manley
Nancy R
NR
D000818:Animals; D016895:Culture Media, Serum-Free; D046508:Culture Techniques / Q000379:methods; D004622:Embryo, Mammalian / Q000502:physiology*; D017403:In Situ Hybridization; D051379:Mice
D016895:Culture Media, Serum-Free