The role of CCL22 (MDC) for the recruitment of eosinophils during allergic pleurisy in mice.
Eosinophils are important inflammatory cells in allergic diseases. In the present study, we have investigated the effects of CCL22 on the recruitment of eosinophils in vivo and in vitro. CCL22 induced a dose- and time-dependent recruitment of eosinophils into the pleural cavity of mice, and this was dependent on the release of platelet-activating factor (PAF) and subsequent generation of CCL11. However, in an allergic pleurisy model, an anti-CCL22 polyclonal antibody given during sensitization or before challenge had no significant effect on eosinophil recruitment. CCL22 did not induce eosinophil chemotaxis in vitro but was able to induce eosinophil degranulation in vitro and in vivo. In conclusion, we show that although exogenously added CCL22 may induce eosinophil migration in vivo via release of PAF and CCL11 (eotaxin), endogenous production of CCL22 does not drive eosinophil migration during allergic inflammation. However, CCL22 may be an important activator of eosinophils once these cells have migrated into tissue.
Pinho
Vanessa
V
Immunopharmacology, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Oliveira
Sandra H
SH
Souza
Danielle G
DG
Vasconcelos
Denise
D
Alessandri
Ana Letícia
AL
Lukacs
Nicholas W
NW
Teixeira
Mauro M
MM
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects; D054413:Chemokine CCL11; D054422:Chemokine CCL22; D019742:Chemokines, CC / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology* / Q000502:physiology; D002631:Chemotactic Factors, Eosinophil / Q000276:immunology / Q000502:physiology; D002633:Chemotaxis / Q000187:drug effects*; D004804:Eosinophils / Q000166:cytology*; D006967:Hypersensitivity / Q000473:pathology; D007975:Leukotriene B4 / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D010980:Platelet Membrane Glycoproteins / Q000235:genetics / Q000502:physiology; D010998:Pleurisy / Q000276:immunology / Q000473:pathology*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology; D043562:Receptors, G-Protein-Coupled
D000906:Antibodies; C515457:Ccl11 protein, mouse; C515483:Ccl22 protein, mouse; D054413:Chemokine CCL11; D054422:Chemokine CCL22; D019742:Chemokines, CC; D002631:Chemotactic Factors, Eosinophil; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor; D007975:Leukotriene B4