3-Nitrotyrosine in atherosclerotic blood vessels.
The effect of peroxynitrite on the development of atherosclerosis is one of the major foci of recent studies. Here, the cytotoxic effect of peroxynitrite was investigated by quantitatively measuring nitrated tyrosine, 3-nitrotyrosine (3-NT) levels in atherosclerotic blood vessels. Atherosclerotic vessels were obtained from the patients who underwent either coronary artery or peripheric artery bypass surgery. Internal thoracic arteries of the patients were treated as non-atherosclerotic control vessels. 3-NT was measured by reverse-phase HPLC and plasma nitrite-nitrate levels were measured by spectrophotometry. 3-NT levels were significantly elevated in atherosclerotic vessels (46.6 +/- 23.3 nmol/mg protein, n = 15; p < 0.001) in comparison to control vessels (15.8 +/- 2.5 nmol/mg protein, n = 10). Vessel 3-NT correlated weakly with plasma nitrate levels (r = 0.38). Thus, atherosclerotic arteries have higher 3-NT levels than non-atherosclerotic blood vessels.
Sucu
Nehir
N
Department of Thoracic and Cardiovascular Surgery, Faculty of Medicine, Mersin University, Turkey. nehirsucu@superonline.com
Unlü
Ali
A
Tamer
Lülüfer
L
Aytacoglu
Barlas
B
Ercan
Bahadir
B
Dikmengil
Murat
M
Atik
Ugur
U
D001161:Arteriosclerosis / Q000378:metabolism* / Q000473:pathology; D001808:Blood Vessels / Q000378:metabolism*; D016022:Case-Control Studies; D006801:Humans; D025202:Molecular Diagnostic Techniques; D009566:Nitrates / Q000032:analysis / Q000097:blood; D009573:Nitrites / Q000032:analysis / Q000097:blood; D013053:Spectrophotometry; D013895:Thoracic Arteries / Q000378:metabolism* / Q000473:pathology; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism*; D015854:Up-Regulation
D009566:Nitrates; D009573:Nitrites; C002744:3-nitrotyrosine; D014443:Tyrosine