Cloning, expression, and genomic structure of a novel human Rap2 interacting gene (RPIP9).
During a large-scale screen of a human fetal brain cDNA library, a full-length cDNA encoding a novel Rap2 interacting protein was isolated and sequenced. The cDNA is 3397bp long and has a predicted open reading frame encoding a protein of 329 aa. The predicted protein shows high homology to mouse and human RPIP8, and has a RUN domain near its C-terminus. The gene was mapped to human chromosome 7q21-7q22 and has 9 exons and 8 introns. The expression pattern was also detected by cycle-limited reverse polymerase chain reaction (RT-PCR).
Wang
Shu
S
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, People's Republic of China.
Zhang
Zhen
Z
Ying
Kang
K
Chen
Jin-zhong
JZ
Meng
Xian-fang
XF
Yang
Quan-Sheng
QS
Xie
Yi
Y
Mao
Yu-min
YM
D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D002897:Chromosomes, Human, Pair 7; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism*; D009928:Organ Specificity; D020161:Physical Chromosome Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D020668:rap GTP-Binding Proteins / Q000378:metabolism*
D002352:Carrier Proteins; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; C477805:RUNDC3B protein, human; C116756:RAP2A protein, human; C492137:Rap2a protein, mouse; D020668:rap GTP-Binding Proteins