title: Attenuated mild colonic inflammation and improved survival from severe DSS-colitis of transgenic Cu/Zn-SOD mice.
abstract: Mucosal tissue damage in chronic inflammatory bowel disease (IBD) is partly caused by an enduring exposure to excessive amounts of reactive oxygen metabolites (ROM). To protect themselves from the toxic effects of ROM, most intestinal cell types constitutively express the highly specific, key ROM-neutralizing cytosolic enzyme Cu/Zn-superoxide dismutase (SOD). Under inflammatory conditions, however, its protein and activity levels have consistently been reported as being decreased. To elucidate a direct functional relationship between intracellular Cu/Zn-SOD expression and intestinal inflammation, we investigated the effects of transgenic human Cu/Zn-SOD overexpression in acute and chronic murine dextran sodium sulfate (DSS)-induced colitis. When subjected to a mild form of acute colitis, the Cu/Zn-SOD overexpressing mice showed a significantly lower colonic activity of neutrophilic myeloperoxidase (MPO) than their nontransgenic littermates. This difference was particularly evident in the male animals. In contrast, a severe acute colitis did not lead to any differences in MPO activity between both groups. Yet, when the animals were subsequently allowed to recover, MPO levels were again significantly lower in the transgenes, suggesting an involvement of Cu/Zn-SOD in, particularly, the clearance of neutrophils. Specific, immunohistochemical identification of neutrophils confirmed the validity of the MPO activity measurements. In addition, transgenic animals showed a remarkable survival benefit from severe DSS colitis over their nontransgenic littermates, particularly during or shortly after the acute inflammatory phase. During the chronic inflammatory phase, which was not characterized by massive neutrophil infiltration, no effects of Cu/Zn-SOD overexpression were noted. Paradoxically, overexpression of Cu/Zn-SOD did not obviously improve the colitis-related (oxidative) injury or symptoms at any stage of the experiment. Surprisingly, however, we did observe a pronounced male gender preference for DSS susceptibility that was reflected by increased male colitis mortality. Our findings provide direct in vivo evidence for a protective, neutrophil-related role for Cu/Zn-SOD in intestinal inflammation. As such, they support the concept of SOD-based (adjunct) antioxidant treatment strategies for inflammatory bowel disease.
authors.affiliation: Department of Gastroenterology-Hepatology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Laurens
authors.initials: L
authors.lastname: Kruidenier
authors.forename: Marieke E
authors.initials: ME
authors.lastname: van Meeteren
authors.forename: Ineke
authors.initials: I
authors.lastname: Kuiper
authors.forename: Dick
authors.initials: D
authors.lastname: Jaarsma
authors.forename: Cornelis B H W
authors.initials: CB
authors.lastname: Lamers
authors.forename: Freek J
authors.initials: FJ
authors.lastname: Zijlstra
authors.forename: Hein W
authors.initials: HW
authors.lastname: Verspaget
mesh_terms: D000208:Acute Disease; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002908:Chronic Disease; D003092:Colitis / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology*; D003106:Colon / Q000201:enzymology; D017931:DNA Primers / Q000737:chemistry; D016264:Dextran Sulfate / Q000633:toxicity; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D007249:Inflammation / Q000201:enzymology*; D008297:Male; D008657:Metabolic Clearance Rate; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009504:Neutrophils / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D016133:Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D015996:Survival Rate
chemical_list: D000975:Antioxidants; D017931:DNA Primers; D007202:Indicators and Reagents; D016264:Dextran Sulfate; D002374:Catalase; D009195:Peroxidase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase