Helicobacter pylori and Campylobacter rectus share a common antigen.
OBJECTIVE
The aim of this study was to investigate the presence of antigens with immunological cross-reactivity in periodontopathogenic bacteria and Helicobacter pylori, the pathogen associated with gastritis and peptic ulcers in human.


RESULTS
Among the putative periodontopathogens tested (Actinobacillus actinomycetemcomitans, Campylobacter rectus, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia and Treponema denticola), cross-reactive bands were only detected in C. rectus by SDS-PAGE/Western immunoblotting analysis using a polyclonal antibody directed to H. pylori cells. One of these cross-reactive antigens, a 64-kDa band antigen, also reacted with a monoclonal antibody directed to the human heat shock protein (HSP) 60. The N-terminal amino acid sequence of this C. rectus protein revealed a high degree of homology with corresponding regions of other HSPs belonging to the HSP60 family, indicating that the 64-kDa antigen was a GroEL protein. The nucleotide sequence of the C. rectus GroEL protein coded for a 547 amino acid protein with a predicted size of 57.8 kDa. Comparison of the alignment of the deduced amino acid sequence of the GroEL protein of C. rectus with that of H. pylori showed a high degree of similarity throughout its length (76.8%). GroEL protein from C. rectus possessed the ability to stimulate production of IL-6 by a confluent monolayer of human gingival epithelial cells and was cytotoxic when used at a high concentration.


CONCLUSIONS
This study reveals an immunological relationship between H. pylori and C. rectus, and clearly indicates that one of the shared antigens is a GroEL protein possessing a biological activity that might play a role in the initiation and progression of periodontal disease.
Tanabe
S
S
Department of Preventive Dentistry, School of Dentistry, The University of Tokushima, Tokushima City, Japan.
Hinode
D
D
Yokoyama
M
M
Fukui
M
M
Nakamura
R
R
Yoshioka
M
M
Grenier
D
D
Mayrand
D
D
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000145:classification* / Q000302:isolation & purification; D015153:Blotting, Western; D002167:Campylobacter / Q000276:immunology*; D002478:Cells, Cultured; D018834:Chaperonin 60 / Q000276:immunology; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000276:immunology; D005881:Gingiva / Q000276:immunology; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D015850:Interleukin-6 / Q000276:immunology; D020539:Sequence Analysis, Protein; D012689:Sequence Homology, Nucleic Acid
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D018834:Chaperonin 60; D015850:Interleukin-6