title: Flow cytometric measurements of neutrophil functions: the dependence on the stimulus to cell ratio.
abstract: Phagocytosis and antimicrobial killing of neutrophils has been quantitatively determined as a function of the stimulus (Candida albicans) to cell ratio R using two donor collectives containing a total of 115 blood samples. Analysis of the collectives in two different laboratories according to the same flow cytometric protocol for simultaneous measurement of neutrophil functions did not produce statistically significant differences. The number of phagocytosing leukocytes as well as that of killed fungi per leukocyte depends strongly on R. While each phagocytosing neutrophil kills one fungus at low values of R, each neutrophil kills on average 2.5 fungi for large R.
authors.affiliation: Institute of Medical Microbiology and Hygiene, Technical University Dresden, Fiedlerstr. 42, 01307 Dresden, Germany. kirsten.anding@mailbox.tu-dresden.de
authors.forename: Kirsten
authors.initials: K
authors.lastname: Anding
authors.forename: Jan M
authors.initials: JM
authors.lastname: Rost
authors.forename: Enno
authors.initials: E
authors.lastname: Jacobs
authors.forename: Franz D
authors.initials: FD
authors.lastname: Daschner
mesh_terms: D000328:Adult; D005434:Flow Cytometry / Q000379:methods*; D005658:Fungi / Q000276:immunology; D006801:Humans; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology* / Q000502:physiology; D010587:Phagocytosis; D016897:Respiratory Burst