title: Role of EphA4 and EphrinB3 in local neuronal circuits that control walking.
abstract: Local circuits in the spinal cord that generate locomotion are termed central pattern generators (CPGs). These provide coordinated bilateral control over the normal limb alternation that underlies walking. The molecules that organize the mammalian CPG are unknown. Isolated spinal cords from mice lacking either the EphA4 receptor or its ligand ephrinB3 have lost left-right limb alternation and instead exhibit synchrony. We identified EphA4-positive neurons as an excitatory component of the locomotor CPG. Our study shows that dramatic locomotor changes can occur as a consequence of local genetic rewiring and identifies genes required for the development of normal locomotor behavior.
authors.affiliation: Department of Medical Biochemistry, Gothenburg University, Medicinaregatan 9 A, 405 30 Gothenburg, Sweden. klas.kullander@medkem.gu.se
authors.forename: Klas
authors.initials: K
authors.lastname: Kullander
authors.forename: Simon J B
authors.initials: SJ
authors.lastname: Butt
authors.forename: James M
authors.initials: JM
authors.lastname: Lebret
authors.forename: Line
authors.initials: L
authors.lastname: Lundfald
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Restrepo
authors.forename: Anna
authors.initials: A
authors.lastname: Rydström
authors.forename: Rudiger
authors.initials: R
authors.lastname: Klein
authors.forename: Ole
authors.initials: O
authors.lastname: Kiehn
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D001640:Bicuculline / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004594:Electrophysiology; D036389:Ephrin-B3 / Q000235:genetics / Q000502:physiology*; D005684:Gait; D066298:In Vitro Techniques; D007395:Interneurons / Q000502:physiology; D026901:Membrane Transport Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009043:Motor Activity; D009474:Neurons / Q000502:physiology*; D009557:Nipecotic Acids / Q000494:pharmacology; D036122:Receptor, EphA4 / Q000235:genetics / Q000502:physiology*; D012521:Sarcosine / Q000494:pharmacology; D015398:Signal Transduction; D013116:Spinal Cord / Q000502:physiology*; D013126:Spinal Nerve Roots / Q000502:physiology; D013331:Strychnine / Q000494:pharmacology; D050598:Vesicular Glutamate Transport Protein 1; D050599:Vesicular Glutamate Transport Protein 2; D033921:Vesicular Transport Proteins; D016138:Walking
chemical_list: D002352:Carrier Proteins; D036389:Ephrin-B3; D026901:Membrane Transport Proteins; D009557:Nipecotic Acids; C493477:Slc17a6 protein, mouse; D050598:Vesicular Glutamate Transport Protein 1; D050599:Vesicular Glutamate Transport Protein 2; D033921:Vesicular Transport Proteins; C030278:nipecotic acid; D036122:Receptor, EphA4; D013331:Strychnine; D001640:Bicuculline; D012521:Sarcosine