Specific conjugation of DNA binding proteins to DNA templates through thiol-disulfide exchange.
The double-stranded oligodeoxyribonucleotides with single internucleotide disulfide linkages were successfully used for covalent trapping of cysteine containing protein. In particular, an efficient conjugation of DNA methyltransferase SsoII to sequence-specific decoys was demonstrated. The obtained results assume that synthetic oligodeoxyribonucleotides bearing a new trapping site can be used as new tools to study and manipulate biological systems.
Metelev
Valeri G
VG
Chemical Department, M V Lomonosov Moscow State University, Vorob'evi gori, Moscow 119992, Russia.
Kubareva
Elena A
EA
Vorob'eva
Olga V
OV
Romanenkov
Andrey S
AS
Oretskaya
Tatiana S
TS
D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004220:Disulfides / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013698:Templates, Genetic
D004268:DNA-Binding Proteins; D004220:Disulfides; D013438:Sulfhydryl Compounds; D004247:DNA