title: Genotyping of four genetic polymorphisms in the CYP1A2 gene in the Egyptian population.
abstract: OBJECTIVE
  The goal of this study was to determine the frequencies of CYP1A2*1C, *1D, *1E and *1F variants in the Egyptian population and compare frequencies with other populations.
  
  
  METHODS
  Genotyping was performed in a total of 212 unrelated Egyptian subjects using polymerase chain reaction-restriction fragment length polymorphism assay.
  
  
  RESULTS
  The frequencies of CYP1A2*1C, *1D, *1E and *1F variants in the Egyptian population were 0.07, 0.40, 0.03 and 0.68, respectively. The Egyptians have a lower frequency of CYP1A2*1C, and CYP1A2*1E than the Japanese (0.07 vs 0.21 and 0.03 vs 0.08, respectively), while the frequencies of CYP1A2*1D and CYP1A2*1F did not differ significantly between the two groups. CYP1A2*1F (0.68) frequency in Egyptians was identical to that observed in Caucasians (0.68 among 236 German individuals).
  
  
  CONCLUSIONS
  The present study is the first to describe the frequencies of four known allelic variants of CYP1A2 among the Egyptian population. CYP1A2*1C and *1E occurred at frequencies significantly lower than that in Japanese, while similar frequencies were observed for CYP1A2*1D and *1F. The CYP1A2*1F frequency appeared to be identical to that of Caucasians. This does not exclude the possibility of the presence of new mutations relatively specific to the Egyptian population that have not been identified.
authors.affiliation: Department of Pharmaceutical Sciences, Tohoku University Hospital, Sendai, Japan.
authors.forename: Samar I
authors.initials: SI
authors.lastname: Hamdy
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hiratsuka
authors.forename: Kaori
authors.initials: K
authors.lastname: Narahara
authors.forename: Naomi
authors.initials: N
authors.lastname: Endo
authors.forename: Mervat
authors.initials: M
authors.lastname: El-Enany
authors.forename: Nadia
authors.initials: N
authors.lastname: Moursi
authors.forename: Mohammed S-E
authors.initials: MS
authors.lastname: Ahmed
authors.forename: Michinao
authors.initials: M
authors.lastname: Mizugaki
mesh_terms: D019388:Cytochrome P-450 CYP1A2 / Q000235:genetics*; D004534:Egypt / Q000208:ethnology; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D009154:Mutation / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D019388:Cytochrome P-450 CYP1A2