title: Effects of 20-hydroxyecdysone and serotonin on neurite growth and survival rate of antennal lobe neurons in pupal stage of the silk moth Bombyx mori in vitro.
abstract: Effects of 20-hydroxyecdysone and serotonin on the morphological development and the survival of antennal lobe neurons from day-2 pupal brains of the silk moth Bombyx mori were investigated in vitro. Four morphologically distinct neuronal types could be identified in the cultured antennal lobe neurons: unipolar, bipolar, multi-polar and projection neurons. Antennal lobe neurons in culture with 20-hydroxyecdysone and serotonin showed different patterns of the morphological development from those described in Manduca sexta. Projection neurons extend their neurites remarkably by 20-hydroxyecdysone in B. mori, but there is no extension from antennal lobe neurons in M. sexta. Multi-polar neurons conspicuously increase only formation of new branches from their primary neurites by serotonin in B. mori, but there are both extension and branching of the neurites in M. sexta. On day-5, antennal lobe neurons in lower titers of 20-hydroxyecdysone had significantly higher survival rates than those in higher titers. Neurons cultured for 7 days at different levels of 20-hydroxyecdysone generally showed significantly lower survival rates than neurons cultured for 5 days under the same conditions.
authors.affiliation: Graduate School of Life Science and Biotechnology, Korea University, Seoul.
authors.forename: Hun Hee
authors.initials: HH
authors.lastname: Park
authors.forename: Cheolin
authors.initials: C
authors.lastname: Park
authors.forename: Kwan Seon
authors.initials: KS
authors.lastname: Kim
authors.forename: Oh Seok
authors.initials: OS
authors.lastname: Kwon
authors.forename: Sung Sik
authors.initials: SS
authors.lastname: Han
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Hwang
authors.forename: Sang Mong
authors.initials: SM
authors.lastname: Lee
authors.forename: Su Il
authors.initials: SI
authors.lastname: Seong
authors.forename: Seok Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: Hak Ryul
authors.initials: HR
authors.lastname: Kim
authors.forename: Bong Hee
authors.initials: BH
authors.lastname: Lee
mesh_terms: D000818:Animals; D012831:Bombyx / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D001921:Brain / Q000166:cytology / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004441:Ecdysterone / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D066298:In Vitro Techniques; D016501:Neurites / Q000187:drug effects*; D009475:Neurons, Afferent / Q000187:drug effects*; D011679:Pupa / Q000166:cytology / Q000187:drug effects*; D012679:Sense Organs / Q000166:cytology / Q000187:drug effects*; D012701:Serotonin / Q000494:pharmacology*
chemical_list: D016166:Free Radical Scavengers; D012701:Serotonin; D004441:Ecdysterone