Assessment of intestinal permeability: enzymatic determination of urinary mannitol, raffinose, sucrose and lactose on Hitachi analyzer.
The sugar absorption test is the usual test for measurement of intestinal permeability. After intestinal absorption of probe sugars the subsequently excreted sugars are measured in urine. We have developed four enzymatic methods for the measurement of the urinary concentration of the probe sugars mannitol, raffinose, lactose and sucrose. Mannitol, lactose and sucrose are directly measured on Hitachi 917 using mannitol dehydrogenase, beta-galactosidase and invertase, respectively, as enzyme reagents. Raffinose measurement needs a three hours preincubation with alpha-galactosidase, after which the liberated sucrose is measured. The analytical performances such as within- and between-run precision, linearity, lowest detection limit, interference of other sugars and comparison with a gas chromatographic method are described for the four methods. These methods are accurate an can easily be performed in any clinical laboratory.
Hessels
Jan
J
Clinical Laboratory, Twenteborg Hospital, Almelo, The Netherlands. j.hessels@twenteborg.nl
Snoeyink
Ellen J M
EJ
Platenkamp
Antonius J
AJ
Voortman
Gerrit
G
Steggink
Jan
J
Eidhof
Harry H M
HH
D002849:Chromatography, Gas; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000201:enzymology; D007785:Lactose / Q000652:urine*; D008353:Mannitol / Q000652:urine*; D008354:Mannitol Dehydrogenases / Q000378:metabolism; D010539:Permeability; D011887:Raffinose / Q000652:urine*; D013053:Spectrophotometry / Q000379:methods; D013395:Sucrose / Q000652:urine*; D043324:beta-Fructofuranosidase; D001616:beta-Galactosidase / Q000378:metabolism
D008353:Mannitol; D013395:Sucrose; D008354:Mannitol Dehydrogenases; D006026:Glycoside Hydrolases; D001616:beta-Galactosidase; D043324:beta-Fructofuranosidase; D007785:Lactose; D011887:Raffinose