Epstein-Barr virus regulates STAT1 through latent membrane protein 1.
This study shows a mechanism for the increase of signal transducer and activator of transcription 1 (STAT1) in Epstein-Barr virus-immortalized cells. Latent membrane protein 1 (LMP-1) expression was sufficient to induce STAT1 expression, DNA binding, and transcriptional activity. LMP-1-expressing cells can induce an increase in STAT1 expression in LMP-negative cells in the same culture, suggesting an indirect regulation of STAT1 expression. The increase in STAT1 expression is mediated by the C-terminal activating region 1 (CTAR-1) and/or CTAR-2 domains of LMP-1 and is inhibited by mutant IkappaB, demonstrating a role for NFkappaB in LMP-1-mediated STAT1 expression.
Richardson
Ciarán
C
Infection and Immunity, University of Wales College of Medicine, Heath Park, Cardiff, United Kingdom.
Fielding
Ceri
C
Rowe
Martin
M
Brennan
Paul
P
D001402:B-Lymphocytes / Q000378:metabolism / Q000821:virology; D002460:Cell Line; D002472:Cell Transformation, Viral; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation; D004854:Herpesvirus 4, Human / Q000472:pathogenicity* / Q000502:physiology*; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D017434:Protein Structure, Tertiary; D050794:STAT1 Transcription Factor; D015398:Signal Transduction; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism*; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000502:physiology*
D004268:DNA-Binding Proteins; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D016328:NF-kappa B; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D015534:Trans-Activators; D014763:Viral Matrix Proteins; D004247:DNA