title: High-throughput manual parallel synthesis using SynPhase crowns and lanterns.
abstract: The high-throughput manual solid-phase parallel synthesis of libraries comprising thousands of discrete samples using pellicular supports (i.e. SynPhase crowns and lanterns) and a suite of novel tools and techniques is described. Key aspects of this approach include the combination of a split-split-split synthesis strategy with spatial encoding to differentiate thousands of crowns, the rapid washing and filtration of up to 48 reaction vessels in parallel, the application of an inexpensive and environmentally friendly technique to remove trifluoroacetic acid from sixteen 96-well plates in parallel, and a high-throughput method for removing cleaved crowns from reusable pin racks. Tens of thousands of discrete samples have been produced in-house using this conceptually and operationally straightforward strategy.
authors.affiliation: Department of Medicinal Chemistry, GlaxoSmithKline Inc., Five Moore Drive, P.O. Box 13398, Research Triangle Park, North Carolina 27709, USA. samuel.gerritz@bms.com
authors.forename: Samuel W
authors.initials: SW
authors.lastname: Gerritz
authors.forename: Mark H
authors.initials: MH
authors.lastname: Norman
authors.forename: Lee A
authors.initials: LA
authors.lastname: Barger
authors.forename: Judd
authors.initials: J
authors.lastname: Berman
authors.forename: Eric C
authors.initials: EC
authors.lastname: Bigham
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bishop
authors.forename: David H
authors.initials: DH
authors.lastname: Drewry
authors.forename: Deanna T
authors.initials: DT
authors.lastname: Garrison
authors.forename: Dennis
authors.initials: D
authors.lastname: Heyer
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Hodson
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Kakel
authors.forename: James A
authors.initials: JA
authors.lastname: Linn
authors.forename: Brian E
authors.initials: BE
authors.lastname: Marron
authors.forename: Suganthini S
authors.initials: SS
authors.lastname: Nanthakumar
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Navas