title: In vitro culture of human bone marrow mesenchymal stem cell clonies and induced differentiation into neuron-like cells.
abstract: OBJECTIVE
  To study the long-term in vitro culture of human bone marrow mesenchymal stem cells (hMSC) and their phenotypical and functional properties.
  
  
  METHODS
  Adherent hMSC colonies were digested by 0.25% trypsin-EDTA with a clone cycle for in vitro subculture. Flow cytometry was employed to examine the phenotypes of the cells. Their committed differentiation potential to neurons, clone-forming ability and growth curves were all investigated.
  
  
  RESULTS
  hMSCs could be subcultured under this culture condition for 20 passages, expressing CD13, CD29 and CD59 but not CD11, CD14, CD31, CD34, CD45, CD80, CD86, CD117 and HLA-DR. The cells could be induced to differentiate into neurons when subcultured for 17 passages.
  
  
  CONCLUSIONS
  hMSCs can be efficiently expanded under this culture condition, and the colony-derived hMSCs can maintain the differentiation potentials and retain their biological characteristics.
authors.affiliation: Department of Hematology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
authors.forename: Jing-ren
authors.initials: JR
authors.lastname: Lin
authors.forename: Kun-yuan
authors.initials: KY
authors.lastname: Guo
authors.forename: Jiang-qi
authors.initials: JQ
authors.lastname: Li
authors.forename: Ding-an
authors.initials: DA
authors.lastname: Yan
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D009474:Neurons / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology