title: Cationic electrophoresis and electrotransfer of membrane glycoproteins.
abstract: Sodium dodecyl sulfate electrophoresis of glycoproteins results in broad, fuzzy bands. The use of the positively charged detergent cetyltrimethylammonium bromide (CTAB) avoids this problem. Protocols for CTAB electrophoresis, electroblotting, and protein detection are presented. These methods were found useful in studies on the multiple drug resistance ATPase Mdr1.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Kuwait, Safat, 13110, Kuwait. engelbert_buxbaum@web.de
authors.forename: Engelbert
authors.initials: E
authors.lastname: Buxbaum
mesh_terms: D000818:Animals; D015153:Blotting, Western; D016466:CHO Cells; D002412:Cations / Q000737:chemistry*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008562:Membrane Glycoproteins / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling / Q000379:methods
chemical_list: D002412:Cations; D002593:Cetrimonium Compounds; D008562:Membrane Glycoproteins; D012967:Sodium Dodecyl Sulfate; D000077286:Cetrimonium