Enhancer-promoter communication mediated by Chip during Pannier-driven proneural patterning is regulated by Osa.
The GATA factor Pannier activates proneural achaete/scute (ac/sc) expression during development of the sensory organs of Drosophila through enhancer binding. Chip bridges Pannier with the (Ac/Sc)-Daughterless heterodimers bound to the promoter and facilitates the enhancer-promoter communication required for proneural development. We show here that this communication is regulated by Osa, which is recruited by Pannier and Chip. Osa belongs to Brahma chromatin remodeling complexes and we show that Osa negatively regulates ac/sc. Consequently, Pannier and Chip also play an essential role during repression of proneural gene expression. Our study suggests that altering chromatin structure is essential for regulation of enhancer-promoter communication.
Heitzler
Pascal
P
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, 67404 Illkirch Cedex, Strasbourg, France. pascal@igbmc.u-strasbg.fr
Vanolst
Luc
L
Biryukova
Inna
I
Ramain
Philippe
P
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D018257:Helix-Loop-Helix Motifs / Q000502:physiology; D009420:Nervous System / Q000196:embryology / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; C108740:Chi protein, Drosophila; D029721:Drosophila Proteins; D009687:Nuclear Proteins; C089368:OSA protein, Shigella flexneri; D015534:Trans-Activators; D014157:Transcription Factors; C086469:brm protein, Drosophila; C109259:pnr protein, Drosophila