title: ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures.
abstract: Mammals detect temperature with specialized neurons in the peripheral nervous system. Four TRPV-class channels have been implicated in sensing heat, and one TRPM-class channel in sensing cold. The combined range of temperatures that activate these channels covers a majority of the relevant physiological spectrum sensed by most mammals, with a significant gap in the noxious cold range. Here, we describe the characterization of ANKTM1, a cold-activated channel with a lower activation temperature compared to the cold and menthol receptor, TRPM8. ANKTM1 is a distant family member of TRP channels with very little amino acid similarity to TRPM8. It is found in a subset of nociceptive sensory neurons where it is coexpressed with TRPV1/VR1 (the capsaicin/heat receptor) but not TRPM8. Consistent with the expression of ANKTM1, we identify noxious cold-sensitive sensory neurons that also respond to capsaicin but not to menthol.
authors.affiliation: Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: Gina M
authors.initials: GM
authors.lastname: Story
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Peier
authors.forename: Alison J
authors.initials: AJ
authors.lastname: Reeve
authors.forename: Samer R
authors.initials: SR
authors.lastname: Eid
authors.forename: Johannes
authors.initials: J
authors.lastname: Mosbacher
authors.forename: Todd R
authors.initials: TR
authors.lastname: Hricik
authors.forename: Taryn J
authors.initials: TJ
authors.lastname: Earley
authors.forename: Anne C
authors.initials: AC
authors.lastname: Hergarden
authors.forename: David A
authors.initials: DA
authors.lastname: Andersson
authors.forename: Sun Wook
authors.initials: SW
authors.lastname: Hwang
authors.forename: Peter
authors.initials: P
authors.lastname: McIntyre
authors.forename: Tim
authors.initials: T
authors.lastname: Jegla
authors.forename: Stuart
authors.initials: S
authors.lastname: Bevan
authors.forename: Ardem
authors.initials: A
authors.lastname: Patapoutian
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017487:Ankyrins / Q000737:chemistry; D016466:CHO Cells; D015220:Calcium Channels / Q000378:metabolism*; D002211:Capsaicin / Q000494:pharmacology; D002478:Cells, Cultured; D003080:Cold Temperature; D006224:Cricetinae; D005260:Female; D008565:Membrane Proteins / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009475:Neurons, Afferent / Q000378:metabolism*; D009619:Nociceptors / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D051381:Rats; D017207:Rats, Sprague-Dawley; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels; D013823:Thermoreceptors / Q000737:chemistry / Q000378:metabolism*; D050051:Transient Receptor Potential Channels / Q000378:metabolism*
chemical_list: D017487:Ankyrins; D015220:Calcium Channels; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D000074025:TRPA1 Cation Channel; C473350:TRPA1 protein, human; D050052:TRPC Cation Channels; D050051:Transient Receptor Potential Channels; D002211:Capsaicin