Galactose is needed only for expression of co-receptors used by Theiler's murine encephalomyelitis virus as the virus does not directly bind galactose or use the UDP-galactose transporter as a receptor.
Theiler's murine encephalomyelitis virus (TMEV) infects most mammalian cells, but a TMEV receptor has not been identified. Studies have demonstrated that the UDP-galactose transporter (UGT) is critical for TMEV attachment and entry into mammalian cells (Hertzler et al., Virology 286, 336-344, 2001). It was suggested that UGT might function as a TMEV receptor. We have demonstrated that polyclonal rabbit antibodies to human UGT that cross-react with hamster UGT do not block binding to or infection of mammalian cells by either high- or low-neurovirulence TMEV. In addition, incubation of virus with galactose, or blocking galactose on the cell surface with lectins, does not inhibit TMEV binding or infection. Thus, TMEV needs UGT for its transporter activity and galactose for assembly of its co-receptors (attachment factors) but does not bind directly to galactose. Excluding direct involvement of UGT and galactose in TMEV binding and entry provides further insight into how TMEV interacts with the host cell and should facilitate ongoing studies to identify a TMEV receptor.
Reddi
Honey V
HV
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Chicago, IL, USA.|Departments of Neurology, Northwestern University, Chicago, IL, USA.|Department of Neurology, Evanston Hospital, 2650 Ridge Avenue, Evanston, IL 60201, USA.
Kallio
Patricia
P
Departments of Neurology, Northwestern University, Chicago, IL, USA.|Department of Neurology, Evanston Hospital, 2650 Ridge Avenue, Evanston, IL 60201, USA.
Lipton
Howard L
HL
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Chicago, IL, USA.|Department of Neurology, Evanston Hospital, 2650 Ridge Avenue, Evanston, IL 60201, USA.|Departments of Neurology, Northwestern University, Chicago, IL, USA.|Department of Microbiology-Immunology, Northwestern University, Chicago, IL, USA.
D000818:Animals; D000906:Antibodies; D018188:Cardiovirus Infections / Q000378:metabolism / Q000821:virology; D002460:Cell Line; D005690:Galactose / Q000378:metabolism; D037102:Lectins; D009004:Monosaccharide Transport Proteins / Q000276:immunology / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D017945:Theilovirus / Q000378:metabolism* / Q000472:pathogenicity; D014779:Virus Replication; D000099237:UDP-Galactose Translocators
D000906:Antibodies; D005690:Galactose; D037102:Lectins; D009004:Monosaccharide Transport Proteins; D011991:Receptors, Virus; D000099237:UDP-Galactose Translocators