[Effects of nitriles and amides on the growth and the nitrile hydratase activity of the Rhodococcus sp. strain gt1].
Effects of some nitriles and amides, as well as glucose and ammonium, on the growth and the nitrile hydratase (EC 4.2.1.84) activity of the Rhodococcus sp. strain gt1 isolated from soil were studied. The activity of nitrile hydratase mainly depended on carbon and nitrogen supply to cells. The activity of nitrile hydratase was high in the presence of glucose and ammonium at medium concentrations and decreased at concentrations of glucose more than 0.3%. Saturated unsubstituted aliphatic nitriles and amides were found to be a good source of nitrogen and carbon. However, the presence of nitriles and amides in the medium was not absolutely necessary for the expression of the activity of nitrile hydratase isolated from the Rhodococcus sp. strain gt1.
Maksimov
A Iu
AIu
Institute of Ecology and Genetics of Microorganisms, Ural Division, Russian Academy of Sciences, ul. Pushkina 1-57, Perm, 614081 Russia. maks@ecology.psu.ru
Kuznetsova
M V
MV
Ovechkina
G V
GV
Kozlov
S V
SV
Maksimova
Iu G
IuG
Demakov
V A
VA
D000577:Amides; D003470:Culture Media; D005947:Glucose; D006836:Hydro-Lyases / Q000378:metabolism*; D009570:Nitriles; D000644:Quaternary Ammonium Compounds; D012240:Rhodococcus / Q000201:enzymology* / Q000254:growth & development*; D012988:Soil Microbiology
D000577:Amides; D003470:Culture Media; D009570:Nitriles; D000644:Quaternary Ammonium Compounds; D006836:Hydro-Lyases; C048831:nitrile hydratase; D005947:Glucose