[Morphological and functional characteristics of lymphocytes of mothers of children with Down syndrome].
Nuclear chromatine of peripheral blood lymphocytes was studied in 13 women with children suffering from Down's syndrome using optic structural computer analysis. In 12 cases significant increase of nuclear roundness coefficient was determined. Deformation coefficient was determined for heterochromatine structures in 8 cases. Integral optic density of nuclear chromatine was significantly decreased in 12 women. This indicates the reduction of felgen-positive material due to deficiency of its compact fraction (in 11 cases). The activity of lymphocyte cytoplasmic lactate, alpha-glycerophosphate and succinate dehydrogenases (SDG) was studied morphocytochemically in 5 women who had children with the disease. High activity of mitochondrial SDG was determined in all cases which probably indicates disorders in lymphocyte energy state. This is one of the reasons for retention of risk pregnancy. Further research in this area may serve as a base for complete cytoanalysis in order to distinguish risk groups among women including primagravida for consequent determination of embryonal karyotype.
Novikov
V D
VD
Medical Institute of Novosibirsk, Institute of Clinical and Experimental Lymphology, Siberian Section of RAMS, Novosibirsk.
Valova
T A
TA
Iasakova
N T
NT
Belan
I B
IB
D000328:Adult; D002467:Cell Nucleus / Q000737:chemistry; D002648:Child; D002843:Chromatin / Q000737:chemistry / Q000648:ultrastructure*; D004396:Coloring Agents / Q000032:analysis; D003593:Cytoplasm / Q000737:chemistry; D004314:Down Syndrome / Q000235:genetics*; D005260:Female; D005994:Glycerophosphates / Q000097:blood; D006801:Humans; D007091:Image Processing, Computer-Assisted; D019344:Lactic Acid / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000648:ultrastructure*; D008423:Maternal Age; D008875:Middle Aged; D009035:Mothers; D011247:Pregnancy; D012307:Risk Factors; D012394:Rosaniline Dyes; D013385:Succinate Dehydrogenase / Q000097:blood
D002843:Chromatin; D004396:Coloring Agents; C007722:Feulgen stain; D005994:Glycerophosphates; D012394:Rosaniline Dyes; D019344:Lactic Acid; C029620:alpha-glycerophosphoric acid; D013385:Succinate Dehydrogenase