title: MRP1-transfected cells do not show increased resistance against oxidative stress.
abstract: Sensitivity of V79 Chinese hamster cells and V79 cells transfected with human MRP1 gene to several agents inducing oxidative stress was compared. Cells overexpressing MRP1 did not show increased resistance to tert-butyl hydroperoxide, diamide, paraquat, menadione, dichromate and carmustine as estimated by cell survival and DNA damage assessed by comet assay. These findings suggest that overexpression of MRP1 does not confer increased resistance to oxidative stress.
authors.affiliation: Department of Molecular Biophysics, University of Lódź, 90-237 Lódź, Poland. kbm@biol.uni.lodz.pl
authors.forename: Aneta
authors.initials: A
authors.lastname: Balcerczyk
authors.forename: Błazej
authors.initials: B
authors.lastname: Rychlik
authors.forename: Marcin
authors.initials: M
authors.lastname: Kruszewski
authors.forename: Brian
authors.initials: B
authors.lastname: Burchell
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
mesh_terms: D000818:Animals; D001692:Biological Transport; D015153:Blotting, Western; D001643:Bridged Bicyclo Compounds / Q000378:metabolism; D016466:CHO Cells; D002330:Carmustine / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival; D020552:Comet Assay; D006224:Cricetinae; D004249:DNA Damage; D003958:Diamide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics* / Q000502:physiology*; D018384:Oxidative Stress; D010269:Paraquat / Q000494:pharmacology; D011192:Potassium Dichromate / Q000494:pharmacology; D014162:Transfection; D024483:Vitamin K 3 / Q000494:pharmacology; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: D001643:Bridged Bicyclo Compounds; D002330:Carmustine; D003958:Diamide; D005978:Glutathione; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D010269:Paraquat; D011192:Potassium Dichromate; D024483:Vitamin K 3; D020122:tert-Butylhydroperoxide; C080120:glutathione-bimane; C421109:multidrug resistance-associated protein 1