Differences in airway-inflammation development by house dust mite and diesel exhaust inhalation among mouse strains.
Three mouse strains (BALB/c, ICR, and C3H/He) were injected intratracheally with house dust mites (Der f) four times at 2-week intervals during exposure to diesel exhaust (DE) or clean air for 8 weeks. Der f treatment caused eosinophilic inflammation and proliferation of goblet cells in the airways of the three strains. DE + Der f caused a further increase of eosinophils in BALB/c and ICR mice, but not in C3H/He mice. DE + Der f significantly increased interleukin (IL)-5; regulated on activation, normal T cell expressed, and presumably secreted (RANTES); eotaxin, monocyte chemotactic protein-1 (MCP-1); and macrophage-inflammatory protein-1 alpha (MIP-1 alpha) in all three strains. However, the protein of IL-5 decreased more in C3H/He mice treated with DE + Der f than in mice treated with Air + Der f. The levels of IL-5 in lung tissues corresponded to the pathological changes by Der f and/or DE treatment. The levels of MCP-1 and MIP-1 alpha in the three strains corresponded to the accumulation of lymphocytes in the airway. The adjuvant effect of DE on IgG1 production was observed in the ICR and C3H/He mice. These results suggest that the murine strain differences in the production of eosinophilic airway inflammation by DE + Der f is related to differences in local expression of IL-5, eotaxin, and IgG1 production. The enhancing effects of DE exposure may be mediated mainly by local IL-5.
Ichinose
Takamichi
T
Department of Health Sciences, Oita University of Nursing and Health Sciences, 2944-9 Notsuharu, Oita 870-1201, Japan.
Takano
Hirohisa
H
Sadakane
Kaori
K
Yanagisawa
Rie
R
Kawazato
Hiroaki
H
Sagai
Masaru
M
Shibamoto
Takayuki
T
D000280:Administration, Inhalation; D000818:Animals; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008813:Mice, Inbred ICR; D011014:Pneumonia / Q000209:etiology* / Q000473:pathology; D039981:Pyroglyphidae; D013045:Species Specificity; D001335:Vehicle Emissions / Q000633:toxicity*
D018925:Chemokines; D016207:Cytokines; D001335:Vehicle Emissions