The relative ligand binding preference of the murine ileal lipid binding protein.
The ileal lipid binding protein (ILBP), a member of the intracellular lipid binding protein family, is a 14-kDa protein that has bile and fatty acids as possible physiological ligands. The ligand binding specificity of this protein is not well characterized. Therefore, we studied the lipid binding activity of purified recombinant murine ILBP (mILBP) in vitro. These studies demonstrated by direct analysis the interaction of mILBP with naturally occurring bile and fatty acids. The rank order of binding preference for fatty acids, or unconjugated and conjugated bile acids, was assessed. Among fatty acids, mILBP preferred species that had longer chain length and increased saturation, similar to other members of the intracellular lipid binding protein family. Among the bile acids, mILBP showed the greatest preference for conjugated species that contained a doubly hydroxylated steroid moiety. The results demonstrate that mILBP exhibits a preference for certain species of bile and fatty acids.
Labonté
Eric D
ED
Department of Biochemistry, 328 Heritage Medical Research Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2S2.
Li
Qianqian
Q
Kay
Cyril M
CM
Agellon
Luis B
LB
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D029363:Organic Anion Transporters, Sodium-Dependent; D011485:Protein Binding; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D027981:Symporters; D013656:Taurocholic Acid / Q000378:metabolism
D001647:Bile Acids and Salts; D002352:Carrier Proteins; D005227:Fatty Acids; D008024:Ligands; D029363:Organic Anion Transporters, Sodium-Dependent; D011994:Recombinant Proteins; D027981:Symporters; C071908:sodium-bile acid cotransporter; D013656:Taurocholic Acid