Effects of hyperthermia on rat hippocampal pyramidal cell apoptosis in vitro.
OBJECTIVE
To investigate the changes in the morphology and biochemistry of neonatal rat hippocampal pyramidal cells (HPCs)apoptosis in high-temperature environment.


METHODS
In vitro culture of HPCs was established and then subjected to environmental stimulation with high temperatures (37, 40, and 42 degrees Celsius respectively) for 1 h. All the cells were collected at different time points after the treatment and examined by flow cytometery, fluorescence staining, agarose gel electrophoresis and in situ cell apoptosis detection kit.


RESULTS
The apoptotic rate and the number of apoptotic bodies increased as the temperature was elevated. DNA ladder was presented on agarose gel electrophoresis, and the germination of the apoptotic body was observed by fluorescence microscope.


CONCLUSIONS
Hyperthermia may induce the apoptosis in HPCs in vitro, and the extend of apoptosis and apoptotic behavior may vary with the temperature and treatment duration.
Chen
Guang-zZhong
GZ
Department of High-temperature Medicine, First Military Medical University, Guangzhou 510515, China.
Luo
Bing-dDe
BD
Wang
Hong-qQin
HQ
Zou
Fei
F
Wan
Wei-rRen
WR
Shen
Xiao-hHong
XH
D000818:Animals; D017209:Apoptosis; D001831:Body Temperature; D002478:Cells, Cultured; D005260:Female; D006624:Hippocampus / Q000166:cytology*; D006979:Hyperthermia, Induced / Q000009:adverse effects*; D008297:Male; D017966:Pyramidal Cells / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley