Hemin: a possible cause of oxidative stress in blood circulation of beta-thalassemia/hemoglobin E disease.
A correlation between endogenous hemin and pro-oxidant activity was revealed in serum of beta-thalassemia/hemoglobin E disease (beta-thal/Hb E), which is the most common prevalent type of thalassemia in Thailand. The technique of low temperature electron spin resonance spectroscopy was used for characterization and quantification of high spin ferric heme, which had been identified as hemin (iron (III)-protoporphyrin IX). Hemin was present at levels ranging from 50 to 280 microM in serum of beta-thal/Hb E but not detectable in serum of non-thalassemia. Pro-oxidant activity in serum of beta-thal/Hb E was demonstrated by luminol-mediated chemiluminescence, a sensitive method for screening of free radical generation in vitro. In the presence of H2O2, the chemiluminescence intensity (CL) was about 20 fold enhanced in serum of beta-thal/Hb E, indicating its extensive pro-oxidant activity. The CL showed a good correlation with serum heroin, r = 0.778 (p < 0.001), while the correlations with total serum iron and serum ferritin were 0.260 (p = 0.259) and 0.519 (p = 0.004), respectively. Our finding suggested that serum hemin readily catalyzed free radical reactions and it may contribute a major pro-oxidant in blood circulation of beta-thal/Hb E.
Phumala
Noppawan
N
Department of Pharmacology, Faculty of Science, Mahidol University, Rama 6 Rd, Bangkok 10400, Thailand. scnpm@mahidol.ac.th
Porasuphatana
Supatra
S
Unchern
Supeenun
S
Pootrakul
Pensri
P
Fucharoen
Suthat
S
Chantharaksri
Udom
U
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005260:Female; D005609:Free Radicals; D006427:Hemin / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D006446:Hemoglobin E / Q000096:biosynthesis*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007501:Iron / Q000378:metabolism / Q000494:pharmacology; D015227:Lipid Peroxidation; D008297:Male; D016877:Oxidants / Q000737:chemistry / Q000378:metabolism; D018384:Oxidative Stress; D013997:Time Factors; D024502:alpha-Tocopherol / Q000378:metabolism; D017086:beta-Thalassemia / Q000097:blood*
D005609:Free Radicals; D016877:Oxidants; D006427:Hemin; D006446:Hemoglobin E; D006861:Hydrogen Peroxide; D007501:Iron; D024502:alpha-Tocopherol