FcgammaRIIIA-158V and rheumatoid arthritis: a confirmation study.
OBJECTIVE
To develop a robust assay for genotyping the FcgammaRIIIA-158V/F polymorphism and to confirm the putative association between the FcgammaRIIIA-158V allele and rheumatoid arthritis (RA).


METHODS
This allelic association study examined the FcgammaRIIIA-158V/F polymorphism for association with RA. A novel single-stranded conformational polymorphism assay was used to genotype 828 RA patients and 581 controls from the UK.


RESULTS
The FcgammaRIIIA-158V allele was associated with both RA (P=0.02) and nodules (P=0.04). Individuals homozygous for this higher affinity allele had a significantly increased risk of RA (OR 1.53, 95% CI 1.08-2.18) and the development of nodules (OR 2.20, 95% CI 1.20-4.01). There was no evidence of an interaction with the shared epitope.


CONCLUSIONS
We have developed a novel assay to genotype the FcgammaRIIIA-158F/V polymorphism and confirmed that homozygosity for the FcgammaRIIIA-158V allele is associated with UK Caucasian RA, particularly in those individuals with nodules, suggesting FcgammaRIIIA may play a role in determining disease severity or in the development of nodules per se.
Morgan
A W
AW
Rheumatology and Rehabilitation Research Unit, University of Leeds, UK. a.w.morgan@leeds.ac.uk
Keyte
V H
VH
Babbage
S J
SJ
Robinson
J I
JI
Ponchel
F
F
Barrett
J H
JH
Bhakta
B B
BB
Bingham
S J
SJ
Buch
M H
MH
Conaghan
P G
PG
Gough
A
A
Green
M
M
Lawson
C A
CA
Pease
C T
CT
Markham
A F
AF
Ollier
W E R
WE
Emery
P
P
Worthington
J
J
Isaacs
J D
JD
D000293:Adolescent; D000328:Adult; D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000235:genetics*; D015331:Cohort Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006684:HLA-DR Antigens / Q000032:analysis; D059811:HLA-DRB1 Chains; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D017452:Receptors, IgG / Q000235:genetics*
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D017452:Receptors, IgG