title: Imaging TOF-SIMS analysis of oligonucleotide microarrays.
abstract: Single strand thiolated oligonucleotide (25-mer) was printed onto chemically modified glass and silicon surfaces. Confirmation of the level of attachment attained in each case was effected through detection by conventional confocal fluorescence microscopy. Both positive-ion and negative ion imaging time-of-flight mass spectra were recorded for the visualization of micro-patterned oligonucleotide arrays. This represents the first report of such detection by this form of mass spectrometry on glass. Ultimately, we are interested in the possibility that imaging time-of-flight secondary ion mass spectrometry can discern the orientation and conformation of DNA strands present on the surface of a substrate.
authors.affiliation: Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6.
authors.forename: Larisa-Emilia
authors.initials: LE
authors.lastname: Cheran
authors.forename: Dajana
authors.initials: D
authors.lastname: Vukovich
authors.forename: Michael
authors.initials: M
authors.lastname: Thompson
mesh_terms: D006801:Humans; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods