Multi locus fingerprinting of Listeria monocytogenes by sequence-specific labeling of DNA probes combined with array hybridization.
We have developed an alternative multi locus sequence typing (MLST) approach that targets the variable genetic changes directly in a DNA array format. Our approach is based on DNA array hybridization in combination with sequence-specific labeling of oligonucleotide probes. Listeria monocytogenes was chosen for the development and evaluation of the assay. The genes hlyA, iap, flaA, inlA and actA were targeted. Twenty-nine suitable probe regions were identified within these genes. The DNA array results from 32 different strains were compared to serotype and amplified fragment length polymorphism data. This comparison showed that our DNA array method gave good discrimination between the strains analyzed. In conclusion, the DNA array-based MLST method is a promising tool for fingerprint bacteria.
Rudi
Knut
K
MATFORSK, Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway. knut.rudi@matforsk.no
Katla
Tone
T
Naterstad
Kristine
K
D015373:Bacterial Typing Techniques / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis*; D005798:Genes, Bacterial; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes; D010802:Phylogeny; D012703:Serotyping
D004269:DNA, Bacterial; D015345:Oligonucleotide Probes