Mannose receptor determination by an ELISA-like method.
Mannose receptor determination may be a useful tool in research, because endocytosis via this animal lectin is involved in many functions of macrophage cells, in particular, the scavenger activity, the specific and unspecific defence against infective diseases, the recognition of neoplastic cells and the activation/differentiation process of the monocyte/macrophage and microglial population. To date, available tests required expensive equipment, the use of radioactive material or the availability of a specific antiserum. We describe an ELISA-like assay, based on biotinylated mannose-conjugated bovine serum albumin (BSA), which specifically binds to the cell mannose receptor. Biotin-labelled receptors can be quantified colorimetrically, utilising an avidin-alkaline phosphatase conjugate as the indicator enzyme. This new method is sensitive and reproducible, as well as simple and rapid, and can be performed with standard laboratory equipment.
Musiani
Silvia
S
Department of Experimental Pathology, University of Bologna, Via San Giacomo 14, 40126, Bologna, Italy.
Battelli
Maria Giulia
MG
D000818:Animals; D002417:Cattle; D002460:Cell Line; D003124:Colorimetry / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D037181:Lectins, C-Type / Q000032:analysis* / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000032:analysis* / Q000378:metabolism*; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000378:metabolism*
D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D012710:Serum Albumin, Bovine