OM197-MP-AC induces the maturation of human dendritic cells and promotes a primary T cell response.
Dendritic cell (DC) maturation is critical for the induction of antigen-specific T lymphocyte responses and may be essential for the development of human vaccines relying on T cell immunity. We investigated the effects on human DC of OM-197, a synthetic pseudodipeptide derived from amino acids, linked to three fatty acid chains and devoid of endotoxin properties. OM-197 upregulated the expression of HLA-DR, CD80, CD86, CD83, CD40 and CD54 at the surface of myeloid DC naturally present in blood as well as of DC generated in vitro from monocytes using IL-4 and GM-CSF. OM-197 also induced the release of IL-12 and TNF-alpha from DC. Finally, DC incubated with OM-197 after pulsing with hepatitis B surface antigen (HBs Ag) induced in vitro expansion of IFN-gamma-secreting HBs Ag-specific CD4(+) T lymphocytes from naive individuals. Taken together, these data identify OM-197 as a potential vaccine adjuvant for the induction of Th1-type responses.
Byl
B
B
Laboratoire d'Immunologie Expérimentale, Université Libre de Bruxelles, Brussels, Belgium.
Libin
M
M
Bauer
J
J
Martin
O R
OR
De Wit
D
D
Davies
G
G
Goldman
M
M
Willems
F
F
D015704:CD4 Antigens / Q000378:metabolism; D003470:Culture Media; D016207:Cytokines / Q000096:biosynthesis / Q000097:blood; D003713:Dendritic Cells / Q000187:drug effects*; D005434:Flow Cytometry; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D007202:Indicators and Reagents; D007371:Interferon-gamma / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D018655:Lymphocyte Count; D010641:Phenotype; D010743:Phospholipids / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
D015704:CD4 Antigens; D003470:Culture Media; D016207:Cytokines; D006514:Hepatitis B Surface Antigens; D007202:Indicators and Reagents; C479638:OM 197-MP-AC; D010743:Phospholipids; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D007371:Interferon-gamma