Isolation and characterization of hemopoietic cells from lungs of allergic mice.
We developed a procedure for the isolation of hemopoietic cells from murine lung. Ovalbumin sensitization and challenge increased the numbers of functionally intact hemopoietic progenitors recovered from digested lung fragments by 80-fold to 120-fold, relative to naive controls. Eosinophil precursors, which are absent in the naive mouse lung, accumulated in the lungs of sensitized/challenged mice. Progenitors in allergic BALB/c mice were recoverable from lung parenchyma, not blood or airways, and were exclusively CD34+. Precursors isolated from allergic lung, unlike those from bone marrow, were inhibited by dexamethasone and were stimulated by prostaglandin D(2). This directly demonstrates that sensitized/challenged lungs accumulate hemopoietic progenitors and precursors, distinct from those in bone marrow.
Gaspar Elsas
Maria Ignez C
MI
Departamento Pediatria, Institute Fernandes Figueira, FIOCRUZ, Rio de Janeiro, Brazil.
Maximiano
Elisabeth S
ES
Joseph
Danielle
D
Bonomo
Adriana
A
Vargaftig
Bernardo Boris
BB
Xavier Elsas
Pedro
P
D000818:Animals; D001681:Biological Assay / Q000379:methods; D001853:Bone Marrow / Q000473:pathology; D002452:Cell Count / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods; D002469:Cell Separation / Q000379:methods*; D004195:Disease Models, Animal; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006967:Hypersensitivity / Q000473:pathology*; D008168:Lung / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C