Oxidants and antioxidants affect the expression of glycodelin.
Glycodelin is a glycoprotein that has immunosuppressive activity. We have shown that K562 cells, hematopoitic progenitor cells, are capable of synthesizing glycodelin peptide (Gp) and, perhaps, contribute to Gp in tissues. In addition, several reproductive and nonreproductive tissues themselves are capable of synthesis of glycodelin. In this study, we report that lipid peroxides induce the synthesis of Gp. Antioxidants vitamin E and pyrrolidine dithiocarbamate (PDTC) and antioxidizing enzymes catalase and superoxide dismutase (SOD) effectively blocked phorbol myristate acetate- (PMA-) and lyso phosphatidic acid- (LPA-) induced synthesis of Gp. Dioctanoin (a mimic of diacylglycerol) activated Gp synthesis, and an inhibitor of protein kinase C (PKC) downregulated the response. Based on these observations, we postulate that oxidants by way of PKC might potentiate the angiogenic process.
Ramachandran
Sumathi
S
Emory Center for Advanced Research on Women's Health, Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA 30322, USA.
Rong
Rong
R
Parthasarathy
Sampath
S
D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D004797:Enzyme-Linked Immunosorbent Assay; D000071816:Glycodelin; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry; D006801:Humans; D007166:Immunosuppressive Agents; D020014:K562 Cells; D019787:Linoleic Acid / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D009389:Neovascularization, Pathologic; D016877:Oxidants / Q000737:chemistry / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D011257:Pregnancy Proteins / Q000096:biosynthesis* / Q000737:chemistry; D011759:Pyrrolidines / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000378:metabolism; D013859:Thiocarbamates / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism
D000975:Antioxidants; D000071816:Glycodelin; D006023:Glycoproteins; D007166:Immunosuppressive Agents; D008054:Lipid Peroxides; D008077:Lipoproteins, LDL; D016877:Oxidants; C045246:PAEP protein, human; D011257:Pregnancy Proteins; D011759:Pyrrolidines; D013859:Thiocarbamates; D014810:Vitamin E; C020972:pyrrolidine dithiocarbamic acid; D019787:Linoleic Acid; D002374:Catalase; D013482:Superoxide Dismutase; D010100:Oxygen