title: Essential roles of lipoyl domains in the activated function and control of pyruvate dehydrogenase kinases and phosphatase isoform 1.
abstract: Four pyruvate dehydrogenase kinase and two pyruvate dehydrogenase phosphatase isoforms function in adjusting the activation state of the pyruvate dehydrogenase complex (PDC) through determining the fraction of active (nonphosphorylated) pyruvate dehydrogenase component. Necessary adaptations of PDC activity with varying metabolic requirements in different tissues and cell types are met by the selective expression and pronounced variation in the inherent functional properties and effector sensitivities of these regulatory enzymes. This review emphasizes how the foremost changes in the kinase and phosphatase activities issue from the dynamic, effector-modified interactions of these regulatory enzymes with the flexibly held outer domains of the core-forming dihydrolipoyl acetyl transferase component.
authors.affiliation: Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506, USA. bchter@ksu.edu
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Roche
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Hiromasa
authors.forename: Ali
authors.initials: A
authors.lastname: Turkan
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Gong
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Yan
authors.forename: Shane A
authors.initials: SA
authors.lastname: Kasten
authors.forename: Haiying
authors.initials: H
authors.lastname: Bao
authors.forename: Jianchun
authors.initials: J
authors.lastname: Dong
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D004789:Enzyme Activation; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D017434:Protein Structure, Tertiary; D021122:Protein Subunits; D011769:Pyruvate Dehydrogenase (Lipoamide)-Phosphatase / Q000737:chemistry / Q000378:metabolism*; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase
chemical_list: D007527:Isoenzymes; D021122:Protein Subunits; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D011769:Pyruvate Dehydrogenase (Lipoamide)-Phosphatase; D002118:Calcium