Sterile isolation and cryopreservation of human adherent monocytes/macrophages from mononuclear cell apheresis preparations.
There is evidence from animal and human study that suggests clinical use of monocytes/macrophages may be of benefit in wound healing, tissue regeneration, and cancer therapy. To facilitate further study, a method was developed for sterile isolation and cryopreservation of adherent monocyte/macrophages from mononuclear cell apheresis units collected from unstimulated normal human donors. Preparations contained approximately 1 x 108 total cells and were comprised of approximately 60% monocytes, 38% lymphocytes, and 2% granulocytes. Cells could be cryopreserved for up to 8 months and subsequently thawed and stored at 1-6 degrees C for up to 4 hours with retention of viability and adequate phagocytic function. These cells can be used in clinical trials to determine their possible therapeutic benefit, e.g., whether administration of exogenously supplied cells improves the healing of chronic wounds or promotes the regeneration of transected nerves.
Wagner
Stephen J
SJ
American Red Cross, Biomedical Services, Jerome H. Holland Laboratory for the Biomedical Sciences, Blood & Cell Therapy Development, Rockville, Maryland 20855, USA. wagners@usa.redcross.org
Myrup
Andrew
A
Xu
Yanwen
Y
Katz
Alfred J
AJ
D015925:Cryopreservation / Q000379:methods*; D006801:Humans; D007937:Leukapheresis / Q000379:methods*; D008264:Macrophages; D009000:Monocytes