CpG oligodeoxynucleotides accelerate reovirus type 2-triggered insulitis in DBA/1 suckling mice.
We reported previously that reovirus type-2 (Reo-2) triggers T-helper (Th) 1-mediated autoimmune insulitis resulting in temporal impaired glucose tolerance (IGT) approximately 10 days post infection (d.p.i) in suckling DBA/1 mice. We hypothesized that CpG motifs in bacteria may enhance virus-induced insulitis through its content of unmethylated CpG motifs. In the infected mice, the intraperitoneal treatment of synthetic 20-base oligodeoxynucleotides with CpG motifs (CpG ODN) caused increase in cumulative incidence of insulitis with IGT, increased serum interferon (IFN)-gamma concentration, and high frequency of autoantibody against pancreatic islet cells, compared to the infected mice without CpG ODN at 17 d.p.i. Also CD4+ and CD8+ lymphocytes infiltrated in and/or around pancreatic islets in the CpG ODN-treated mice. This evidence suggests that CpG ODN may contribute to accelerate Reo-2-induced autoimmune reaction against pancreatic islet cells via additional effects of Th1 cytokines especially IFN-gamma.
Hayashi
T
T
Laboratory of Veterinary Pathology, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan. hayashi@agr.yamaguchi-u.ac.jp
Yoshinaka
K
K
Hasegawa
K
K
Maeda
K
K
Onodera
T
T
D000818:Animals; D000833:Animals, Suckling; D001323:Autoantibodies / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D018899:CpG Islands; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology / Q000473:pathology / Q000821:virology*; D005260:Female; D005951:Glucose Tolerance Test; D007371:Interferon-gamma / Q000097:blood; D007515:Islets of Langerhans / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage*; D030102:Orthoreovirus, Mammalian; D012088:Reoviridae Infections / Q000276:immunology* / Q000473:pathology
D001323:Autoantibodies; D009838:Oligodeoxyribonucleotides; D007371:Interferon-gamma