MS screening strategies: investigating the glycomes of knockout and myodystrophic mice and leukodystrophic human brains.
The implementation of highly sensitive and rapid mass spectrometric screening strategies for defining the glycosylation repertoires of organs in knockout mice is helping to reveal the roles that glycans play in health and disease. Thus novel glycosylation pathways have been uncovered in two such knockouts, namely alpha-mannosidase II null mice and UDP-N-acetylglucosamine: alpha 6-D-mannoside beta 1,2-N-acetylglucosaminyltransferase II null mice. This chapter documents the glycosylation profiles of a wide range of organs from the normal mouse which should facilitate future glycomics studies of knockout mice. Furthermore, we report applications of our screening technology in studies of the myodystrophy mouse and a human leukodystrophy.
Sutton-Smith
Mark
M
Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London SW7 2AY, U.K.
Morris
Howard R
HR
Grewal
Prabhjit K
PK
Hewitt
Jane E
JE
Bittner
Reginald E
RE
Goldin
Ehud
E
Schiffmann
Raphael
R
Dell
Anne
A
D000818:Animals; D001921:Brain / Q000378:metabolism*; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D009137:Muscular Dystrophy, Animal / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D012680:Sensitivity and Specificity; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods*; D013106:Sphingolipidoses / Q000378:metabolism*
D011134:Polysaccharides