Measurement of phosphatidylcholine hydroperoxide in mild ischemia-reperfusion injury in rat intestine.
In the pathogenesis of intestinal ischemia-reperfusion injury, the measurement of lipid peroxides needs to be established. Sprague-Dawley rat intestines were assessed after 30 minutes of occlusion of the superior mesenteric artery followed by reperfusion at 30, 60, 120, 180, 360 minutes. Grade of the mucosal injury, accumulation of the activated neutrophils and ICAM-1 expression were transiently increased after reperfusion. Two measuring methods of mucosal lipid peroxides using thiobarbituric acid reacting substance (TBARS) and phosphatidylcholine hydroperoxide (PCOOH) were compared. PCOOH level was significantly increased after reperfusion, while the mucosal TBARS level showed no significant change. In conclusion, lipid peroxidation could be detected with high specificity and sensitivity by measuring the mucosal phosphatidylcholine hydroperoxide level.
Masuko
Tsuyoshi
T
Department of Surgery, Division of GI and Colorectal Surgery, Research Unit of Biological Regulation and Oncology, Tohoku University, Graduate School of Medicine, Sendai 980-8574, Japan.
Funayama
Yuji
Y
Naito
Hiroo
H
Fukushima
Kohei
K
Shibata
Chikashi
C
Takahashi
Ken-ichi
K
Ogawa
Hitoshi
H
Ueno
Tatsuya
T
Hashimoto
Akihiko
A
Miyazawa
Teruo
T
Nakagawa
Kiyotaka
K
Matsuno
Seiki
S
Sasaki
Iwao
I
D000818:Animals; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009504:Neutrophils / Q000473:pathology; D010713:Phosphatidylcholines / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D012680:Sensitivity and Specificity; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
D010713:Phosphatidylcholines; D017392:Thiobarbituric Acid Reactive Substances; C057590:phosphatidylcholine hydroperoxide; D018799:Intercellular Adhesion Molecule-1