title: Allergen-induced bronchial inflammation in house dust mite-allergic patients with or without asthma.
abstract: BACKGROUND
  It is presently unknown which factors determine the occurrence and persistence of asthma in house dust mite-allergic individuals. The level of allergen-specific IgE antibodies does not seem to be decisive for asthmatic symptoms. Moreover, levels of exposure to mite allergens do not seem to differ significantly between asthmatic and non-asthmatics individuals.
  
  
  OBJECTIVE
  It was hypothesized that the presence or absence of asthmatic symptoms in house dust mite-allergic patients is associated with quantitative or qualitative differences in the cellular bronchial inflammatory response during the late phase of the allergic reaction. This hypothesis was tested in the bronchial allergen challenge model.
  
  
  METHODS
  Whole lung challenges with house dust mite extract were performed in 52 house dust mite-allergic subjects, of whom 26 had asthma and 26 had perennial rhinitis without asthmatic symptoms. Primary outcomes were parameters for bronchial inflammation in serial samples of induced sputum (cell differentials, eosinophil cationic protein (ECP), interleukin-8 (IL-8), myeloperoxydase (MPO)). In addition, lung function, non-specific bronchial hyper-responsiveness and serial blood samples (eosinophils and IL-5) were analysed.
  
  
  RESULTS
  At baseline sputum eosinophils and ECP were similar in both groups but neutrophils and IL-8 were higher in asthmatics. The early bronchoconstriction after allergen challenge was similar in asthma and non-asthmatic rhinitis (median decrease in FEV1: asthma -31.7% vs. non-asthmatics -29.1%, P > 0.1). The late phase bronchoconstriction was significantly greater in asthma (median decrease in FEV1: asthma -27.6% vs. non-asthmatics -18.9%, P = 0.02). Induction of bronchial hyper-responsiveness was similar in both groups. Bronchial allergen challenge elicited significant increases in sputum eosinophils and ECP, which were indistinguishable for both groups (P > 0.1 and P = 0.07, respectively). In contrast, higher numbers of neutrophils persisted in asthma 24h after challenge and were accompanied by significant increases in IL-8 and MPO, which were absent in non-asthmatics (difference between groups P = 0.007 and P = 0.05, respectively).
  
  
  CONCLUSIONS
  Allergen challenge inducedvery similar increases in eosinophils and ECP in induced sputum in allergic asthmatics and in allergic non-asthmatic patients. The difference in bronchial inflammation between asthma and non-asthmatic rhinitis appeared to be more closely related to indices for neutrophilic inflammation.
authors.affiliation: Department of Pulmonology, Academic Medical Center, Amsterdam, The Netherlands.
authors.forename: C E
authors.initials: CE
authors.lastname: Lopuhaä
authors.forename: T A
authors.initials: TA
authors.lastname: Out
authors.forename: H M
authors.initials: HM
authors.lastname: Jansen
authors.forename: R C
authors.initials: RC
authors.lastname: Aalberse
authors.forename: J S
authors.initials: JS
authors.lastname: van der Zee
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000009:adverse effects*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology; D001249:Asthma / Q000209:etiology* / Q000276:immunology / Q000503:physiopathology; D016535:Bronchial Hyperreactivity / Q000209:etiology* / Q000276:immunology; D001985:Bronchial Provocation Tests; D016084:Bronchoconstriction; D004391:Dust / Q000276:immunology; D004804:Eosinophils / Q000473:pathology; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D020556:Neutrophil Infiltration; D039981:Pyroglyphidae / Q000276:immunology*; D012221:Rhinitis, Allergic, Perennial / Q000209:etiology* / Q000276:immunology / Q000503:physiopathology; D013183:Sputum / Q000276:immunology
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D004391:Dust