[Hydrodynamic parameters of native C-reactive protein molecule in a solution].
The hydrodynamic properties of the C-reactive protein in solution (pH 6.8) were studied using quasi-elastic light scattering and size-exclusion liquid chromatography. It was shown that the solution containing the C-reactive protein represents a polydisperse system. The values of the translation diffusion coefficient and the apparent molecular weight of the C-reactive protein in solution at pH 6.8 were determined. The values of the translation diffusion coefficient, molecular weight and the hydration radius obtained suggest that the native pentameric C-reactive protein is the major form of the protein in solution at pH 6.8.
Blizniukov
O P
OP
Institute of Immunology, Ministry of Health of Russian Federation, Kashirskoe shosse 24/2, Moscow, 115478 Russia.
Kozmin
L D
LD
Falikova
V V
VV
Titov
V N
VN
Belen'kiĭ
A G
AG
Tishchenko
V M
VM
D002097:C-Reactive Protein / Q000737:chemistry*; D002850:Chromatography, Gel; D006863:Hydrogen-Ion Concentration; D007122:Immunoelectrophoresis; D008027:Light; D008970:Molecular Weight; D011108:Polymers; D012542:Scattering, Radiation; D012996:Solutions
D011108:Polymers; D012996:Solutions; D002097:C-Reactive Protein