Determination of sugar phosphates and nucleotides related to photosynthetic metabolism by high-performance anion-exchange liquid chromatography with fluorometric and ultraviolet detection.
A high-performance anion-exchange liquid chromatography system was constructed to identify sugar phosphates and nucleotides involved in photosynthetic metabolism. First sugar phosphates and nucleotides were separated by a gradient elution with boric acid and sodium phosphate, then they were detected by a fluorescence detector (as fluorescent derivatives with arginine) and UV detector, respectively. Eight authentic sugar phosphates and 11 authentic nucleotides could be analyzed using the system. The applicability of the system to the determination of the corresponding sugar phosphates and nucleotides in extracts from only five soybean leaf discs (8.95 cm2) was shown.
Sawada
Shinichi
S
Department of Biofunctional Science, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, 036-8561, Japan. shinichi@cc.hirosaki-u.ac.jp
Ono
Reiko
R
Sato
Takayuki
T
Suzuki
Satoshi
S
Arakawa
Osamu
O
Kasai
Minobu
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009711:Nucleotides / Q000032:analysis* / Q000378:metabolism; D010788:Photosynthesis; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013403:Sugar Phosphates / Q000032:analysis* / Q000378:metabolism
D009711:Nucleotides; D013403:Sugar Phosphates