title: Birth resulting from transfer of blastocysts cryopreserved with propanediol after spontaneous hatching.
abstract: This paper reports the birth of a healthy baby resulting from transfer of blastocysts that were cryopreserved using propanediol after spontaneous hatching. A young infertile couple underwent IVF treatment in the clinic. After several IVF attempts, two births resulted; the first one with fresh embryos in 1996 after three IVF cycles, and the second one in 1999 (after a new IVF cycle in 1998) with frozen blastocysts that had remained cryopreserved in 1.5 mol/l propanediol and 0.1 mol/l sucrose after spontaneous hatching. This report of a healthy baby following transfer of hatched blastocysts frozen in propanediol supports further exploration of this approach.
authors.affiliation: Halitus Instituto Médico, Marcelo T de Alvear 2084 (C1122AAF), Buenos Aires, Argentina. carlosquint@hotmail.com
authors.forename: C J
authors.initials: CJ
authors.lastname: Quintans
authors.forename: M J
authors.initials: MJ
authors.lastname: Donaldson
authors.forename: M V
authors.initials: MV
authors.lastname: Bertolino
authors.forename: R S
authors.initials: RS
authors.lastname: Pasqualini
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000378:metabolism*; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D011247:Pregnancy; D011409:Propylene Glycols / Q000378:metabolism*
chemical_list: D011409:Propylene Glycols