Coupled oxidation of aromatic hydrocarbons by horseradish peroxidase and hydrogen peroxide.
The oxidation capability of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2) coupled oxidation of aromatic hydrocarbons (o-xylene-d10 and naphthalene-d8) was investigated. Batch experiments were conducted using horseradish peroxidase prepared in potassium phosphate buffer in the presence of H2O2. The oxidation of aromatic hydrocarbon was tested as a function of HRP at a fixed concentration of H2O2, and as a function of the concentration of H2O2 at a constant HRP activity (4000 units/ml). The mass removal of o-xylene-d10 and naphthalene-ds increased with increasing HRP enzymatic activity, and up to 54% and 51% of mass removal were observed for o-xylene-d10 and naphthalene-d8, respectively. Increasing the concentration of H2O2 resulted in increased mass removal of aromatic hydrocarbons.
Fang
Jiasong
J
Department of Civil and Environmental Engineering, The University of Michigan, Ann Arbor, MI 48109, USA. jsfang@iastate.edu
Barcelona
Michael J
MJ
D006735:Horseradish Peroxidase / Q000737:chemistry*; D006841:Hydrocarbons, Aromatic / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D009281:Naphthalenes / Q000737:chemistry; D010084:Oxidation-Reduction; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical; D014992:Xylenes / Q000737:chemistry
D006841:Hydrocarbons, Aromatic; D009281:Naphthalenes; D014874:Water Pollutants, Chemical; D014992:Xylenes; C031721:naphthalene; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C026114:2-xylene