Synchronous onset of oestradiol-17beta secretion by Meishan conceptuses.
The response of Meishan conceptuses to an exogenous precursor for oestradiol-17beta biosynthesis was investigated in vitro, to determine whether gestational age or morphological stage of development elicit changes in hormone metabolism. Conceptuses were recovered on days 11, 12, 13 or 15 after the onset of oestrus and cultured for 6 hours at 37 degrees C, in the presence or absence of testosterone. On days 12 and 13 after the onset of oestrus spherical conceptuses were recovered from some gilts, whereas others yielded elongated or filamentous conceptuses. All conceptuses recovered on day 15 after oestrus had elongated. The number of cells per individual conceptus increased from days 11 to 13 after the onset of oestrus (P < 0.001), as did conceptus surface area (P = 0.038). Supplementing culture media with testosterone, as a substrate for oestrogen biosynthesis, significantly increased conceptus oestradiol-17beta secretion in vitro on days 12, 13 and 15, regardless of whether pre- or post-elongation conceptuses were cultured. However, on day 11 oestradiol-17beta was only detected at significant concentrations in the culture media of four testosterone supplemented conceptuses and only one gilt produced conceptuses capable of secreting oestradiol-17beta in the absence of testosterone. Therefore, the onset of conceptus oestradiol-17beta secretion is apparently limited by the expression of aromatase enzymes that are activated synchronously, irrespective of the stage of morphological development, within Meishan litters. Once established, Meishan conceptus oestradiol-17beta secretion in vitro is increased in the presence of exogenous testosterone.
Pickard
Amanda R
AR
Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen, AB21 9SB, UK. Amanda.Pickard@ioz.ac.uk
Miller
Samuel J
SJ
Ashworth
Cheryl J
CJ
D000818:Animals; D001141:Aromatase / Q000378:metabolism; D001755:Blastocyst / Q000166:cytology; D001947:Breeding; D004622:Embryo, Mammalian / Q000187:drug effects / Q000378:metabolism; D004790:Enzyme Induction; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D005313:Fetal Death / Q000235:genetics / Q000503:physiopathology / Q000662:veterinary; D005865:Gestational Age; D008098:Litter Size; D009924:Organ Culture Techniques; D010060:Ovulation; D011247:Pregnancy; D013552:Swine / Q000196:embryology*; D013739:Testosterone / Q000494:pharmacology
D013739:Testosterone; D004958:Estradiol; D001141:Aromatase