title: The skeletal muscle ryanodine receptor isoform 1 is found at the intercalated discs in human and mouse hearts.
abstract: The ryanodine receptors (RyRs) are a class of intracellular calcium release channels of which there are three isoforms. In striated muscle, isoform 1 and isoform 2 are mainly expressed in the terminal cisternae of sarcoplasmic reticulum of skeletal muscle and heart, respectively. Isoform 3 is widely distributed in tissues but in minuscule amounts. These channels release calcium ions from intracellular stores in excitation-contraction coupling for cell signaling. Here, we report the presence of skeletal muscle isoform 1 localized in the intercalated discs (IDs) of human and mouse hearts. By using RyR1 and connexin43 specific antibodies and dual immunofluorescent techniques, both were localized in the proximity of the IDs of human and mouse hearts. We confirmed that RyR1 is localized to the IDs by selective immunoprecipitation of RyR isoform 1 from a subcellular fraction containing IDs from human heart tissue. The functional significance of our observation remains to be elucidated as isoform 1 is involved in depolarization induced calcium release, unlike RyR isoforms 2 and 3 which appear to be involved in calcium induced calcium release.
authors.affiliation: Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA.
authors.forename: Loice H
authors.initials: LH
authors.lastname: Jeyakumar
authors.forename: Linda A
authors.initials: LA
authors.lastname: Gleaves
authors.forename: Bettye D
authors.initials: BD
authors.lastname: Ridley
authors.forename: Paul
authors.initials: P
authors.lastname: Chang
authors.forename: James
authors.initials: J
authors.lastname: Atkinson
authors.forename: Joey V
authors.initials: JV
authors.lastname: Barnett
authors.forename: Sidney
authors.initials: S
authors.lastname: Fleischer
mesh_terms: D000818:Animals; D000906:Antibodies; D020013:Calcium Signaling / Q000502:physiology*; D018031:Connexin 43 / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D051379:Mice; D009119:Muscle Contraction / Q000502:physiology; D018482:Muscle, Skeletal / Q000378:metabolism*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism*; D013347:Subcellular Fractions
chemical_list: D000906:Antibodies; D018031:Connexin 43; D020033:Protein Isoforms; D019837:Ryanodine Receptor Calcium Release Channel