Cloning of a novel one-repeat calcium channel-like gene.
We describe the cloning of a cDNA from a human testis library that encodes a novel protein with similarity to one repeat of voltage-gated Ca(2+) channels (Ca(v)). Northern and dot blot analyses indicate that the novel Ca(v)-like gene is expressed predominantly in testis and at lower levels in many other tissues. Heterologous expression of the Ca(v)-like protein did not lead to the induction of any detectable ionic current and failed to modify intracellular Ca(2+) concentrations. Similar one-repeat Ca(v)-like proteins have been cloned from Bacillus, Mus, and Homo, and appear to encode ion channels involved in renal function, axis determination, and sperm motility.
Arias
Juan Manuel
JM
Department of Pharmacology, University of Virginia, P.O. Box 800735, 1300 Jefferson Park Avenue, Charlottesville 22908-0735, VA, USA.
Murbartián
Janet
J
Perez-Reyes
Edward
E
D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D004594:Electrophysiology; D005091:Exons; D005260:Female; D015723:Gene Library; D006801:Humans; D007438:Introns; D007473:Ion Channels; D007477:Ions; D008297:Male; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009865:Oocytes; D011485:Protein Binding; D012313:RNA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism; D014018:Tissue Distribution; D014982:Xenopus laevis
D015220:Calcium Channels; D007473:Ion Channels; D007477:Ions; D012313:RNA; D002118:Calcium