Effect of diluents on motility of ram sperm during storage at 5 degrees C.
This study examined the effects of 5 diluents--glucose-phosphate (GP), skim milk-egg yolk (MY), egg yolk-citrate (YC), blood sera (S), and seawater (SW)--on daily motility of ram sperm stored at 5 degrees C for a short time. Ram semen collected by artificial vagina was diluted. Percentage of motile spermatozoa (PM) was assessed every 24 h up to day 10 after dilution. The different kinds of diluents except for SW had no effect on the primary motility of sperm and the primary motility was 0 with SW. There was a significant effect (p<.01) of diluent type and storage times in the conservation rates after cooling for PM. The GP diluent achieved greater values (80.5+/-0.88%) than S (69.5+/-1.70%) and MY (78.0+/-1.17%), while YC showed an intermediate value (79.0+/-1.24%). In relation to time of storage, GP and YC diluents showed the PM values up to day 10, but these values were 8 and 7 days for MY and S, respectively. The GP and YC extenders maintained a PM > 50% up to day 4, while MY was greater than 50% up to day 3 and S to day 2.
Gündoğan
M
M
Department of Reproduction and Artificial Insemination, Afyon Kocatepe University, Faculty of Veterinary Medicine, Afyon, Turkey.
Tekerli
M
M
Uçar
M
M
Türkmenoğlu
I
I
D000818:Animals; D001769:Blood; D019343:Citric Acid / Q000494:pharmacology; D004530:Egg Yolk; D005958:Glucosephosphates / Q000494:pharmacology; D008297:Male; D008892:Milk; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D012623:Seawater; D012662:Semen Preservation; D012756:Sheep; D013081:Sperm Motility / Q000187:drug effects*
D005958:Glucosephosphates; D019188:Organ Preservation Solutions; D019343:Citric Acid