title: Association of ovarian tumor epithelium coexpressing HLA-DR and CA-125 antigens with tumor infiltrating cytotoxic T lymphocytes.
abstract: Understanding of the relationship between tumor infiltrating cytotoxic T lymphocytes (CD 8(+)TILs) and tumor cells as well as tumor-associated antigens is important and may reflect the extent of immune response of the patient to the tumor, thus providing a useful clue relevant to the prognosis. The purpose of this study was to determine the relationship between the expression of HLA-DR and CA-125 antigens and the presence of CD 8(+)TILs with regard to the established clinicopathological factors of ovarian carcinomas using immunohistochemical methods. Thirty-one ovarian carcinomas consisting of 20 serous, 7 mucinous, and 4 clear cell types were examined. Of these, 18 (58%) and 22 (71%) were positive for HLA-DR and CA-125 antigens respectively, and the overall mean number of the CD 8(+)TILs was 7.2+/-2.9. A significant association was observed between the mean number of CD8(+)TILs and tumor grade (P=0.01), disease stage (P=0.003), and patient outcome (P=0.01). The mean number of CD 8(+)TILs in HLA-DR positive (8.6+/-2.2) or CA-125 positive (8.4+/-2.1) tumors was significantly higher than that in HLA-DR negative (5.2+/-2.5; P=0.0003) or CA-125 negative (4.2+/-2.2; P=0.00002) tumors. These significant levels were further enhanced by one order of magnitude when the mean number of CD 8(+)TILs in tumors positive for both HLA-DR and CA-125 antigens (9.1+/-1.7) was compared to that in HLA-DR negative or CA-125 negative tumors. The frequency of cancer-related deaths in HLA-DR and CA-125 positive tumors was significantly lower than in the negative tumors (P=0.01). These data suggest that concurrent expression of HLA-DR and CA-125 antigens may augment the immune response of the patient to the tumor, thus providing a potential clue relevant to the prognosis.
authors.affiliation: Department of Obstetrics and Gynecology, Nippon Medical School, Kanagawa, Japan.
authors.forename: Norihiro
authors.initials: N
authors.lastname: Matsushita
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ghazizadeh
authors.forename: Hideki
authors.initials: H
authors.lastname: Konishi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Araki
mesh_terms: D002288:Adenocarcinoma, Mucinous / Q000276:immunology* / Q000473:pathology*; D018394:CA-125 Antigen / Q000032:analysis*; D018414:CD8-Positive T-Lymphocytes / Q000473:pathology*; D002277:Carcinoma / Q000276:immunology* / Q000473:pathology*; D004848:Epithelium / Q000276:immunology; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D016246:Lymphocytes, Tumor-Infiltrating / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000276:immunology* / Q000473:pathology*
chemical_list: D018394:CA-125 Antigen; D006684:HLA-DR Antigens