Molecular cloning and characterization of mannan binding lectin C gene from Balb/c mice.
OBJECTIVE
To obtain full-length cDNA encoding mouse mannan-binding lectin C(MBL-C) polypeptide.


METHODS
The cDNA encoding mouse MBL-C was isolated from Balb/c mouse liver cells by reverse transcriptase (RT)-PCR and inserted into pUC-T vector, and the encoding region structure of the DNA was analyzed to understand its evolutionary relationship with single human MBL homologues.


RESULTS
The amplified mouse MBL-C cDNA, which was 735 bp in length, encoded 245 amino acid residues, and its structural analysis showed 100% homology with published mouse MBL-C gene sequence and 71.4% homology with MBL gene of Chinese human.


CONCLUSIONS
The gene encoding Balb/c mouse MBL-C has been successfully isolated, which may facilitate further study of the innate immune functions of MBL molecule in vivo.
Wang
Hong-wei
HW
Department of Immunology, First Military Medical University, Guangzhou 510515, China. whwnet@fimmu.com
Chen
Zheng-liang
ZL
Zuo
Da-ming
DM
Lu
Xiao
X
Yu
Xin-pei
XP
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis*; D005260:Female; D006801:Humans; D037601:Mannose-Binding Lectin / Q000031:analogs & derivatives* / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D037601:Mannose-Binding Lectin; C445639:Mbl2 protein, mouse