A soluble protein is immobile in dormant spores of Bacillus subtilis but is mobile in germinated spores: implications for spore dormancy.
Fluorescence redistribution after photobleaching has been used to show that a cytoplasmic GFP fusion is immobile in dormant spores of Bacillus subtilis but becomes freely mobile in germinated spores in which cytoplasmic water content has increased approximately 2-fold. The GFP immobility in dormant spores is not due to the high levels of dipicolinic acid in the spore cytoplasm, because GFP was also immobile in germinated cwlD spores that had excreted their dipicolinic acid but where cytoplasmic water content had only increased to a level similar to that in dormant spores of several other Bacillus species. The immobility of a normally mobile protein in dormant wild-type spores and germinated cwlD spores is consistent with the lack of metabolism and enzymatic activity in these spores and suggests that protein immobility, presumably due to low water content, is a major reason for the metabolic dormancy of spores of Bacillus species.
Cowan
Ann E
AE
Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, CT 06032, USA.
Koppel
Dennis E
DE
Setlow
Barbara
B
Setlow
Peter
P
D001407:Bacillus / Q000235:genetics / Q000502:physiology*; D001412:Bacillus subtilis / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism; D036681:Fluorescence Recovery After Photobleaching; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D010848:Picolinic Acids / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013171:Spores, Bacterial / Q000502:physiology*
D001426:Bacterial Proteins; D008164:Luminescent Proteins; D010848:Picolinic Acids; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; C004860:dipicolinic acid