Clostridium colicanis sp. nov., from canine faeces.
Morphological, biochemical and molecular genetic studies were performed on an unknown, anaerobic, rod-shaped organism isolated from faeces of a canine. The organism was tentatively identified as a member of the genus Clostridium based on its cellular morphology and ability to form endospores but, biochemically, it did not appear to correspond to any recognized species of this genus. Comparative 16S rRNA gene sequence analysis showed that the bacterium represents a previously unrecognized subline within Clostridium rRNA group I (Clostridium sensu stricto), which includes Clostridium butyricum, the type species of the genus. The nearest phylogenetic relatives of the unknown bacterium corresponded to Clostridium absonum, Clostridium baratii, Eubacterium budayi, Eubacterium moniliforme, Eubacterium multiforme and Eubacterium nitritogenes, but 16S rRNA sequence divergence values of > 3% demonstrated that it represents a novel species. Based on the findings presented, a novel species, Clostridium colicanis sp. nov., is described, with the type strain 3WC2T (=CCUG 44556T =DSM 13634T).
Greetham
Hazel L
HL
Food Microbial Sciences Unit, School of Food Biosciences, Whiteknights, PO Box 226, University of Reading, Reading RG6 6AP, UK.
Gibson
Glenn R
GR
Food Microbial Sciences Unit, School of Food Biosciences, Whiteknights, PO Box 226, University of Reading, Reading RG6 6AP, UK.
Giffard
Catriona
C
Waltham Centre for Pet Nutrition, Waltham-on-Wolds, Melton Mowbray, UK.
Hippe
Hans
H
DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
Merkhoffer
Birgit
B
DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
Steiner
Ulrike
U
DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
Falsen
Enevold
E
Culture Collection, Department of Clinical Microbiology, University of Göteborg, Sweden.
Collins
Matthew D
MD
Food Microbial Sciences Unit, School of Food Biosciences, Whiteknights, PO Box 226, University of Reading, Reading RG6 6AP, UK.
D000818:Animals; D001482:Base Composition; D003013:Clostridium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004285:Dogs; D005243:Feces / Q000382:microbiology; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S