Chemosensory proteins of Locusta migratoria.
Two different classes of chemosensory proteins (CSPs) in Locusta migratoria have been identified on the basis of the molecular cloning of a series of different cDNAs from the antennae of this insect. Several CSP isoforms have been purified and biochemically characterized from antennal and wing extracts, some of them corresponding to expression products predicted for the identified cDNAs. In wings, the nature of the main endogenous ligand binding to these proteins was determined as oleoamide by a gas chromatography-mass spectrometric approach. One of these isoforms has been expressed in a bacterial system with high yield and used in a fluorescent binding assay. Competitive binding experiments have indicated the presence of long-chain compounds among the best ligands.
Ban
L
L
Department of Entomology, China Agricultural University, Beijing, China.
Scaloni
A
A
Brandazza
A
A
Angeli
S
S
Zhang
L
L
Yan
Y
Y
Pelosi
Paolo
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002849:Chromatography, Gas; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007915:Lepidoptera / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008024:Ligands; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011485:Protein Binding; D012313:RNA / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D019476:Insect Proteins; D008024:Ligands; D011994:Recombinant Proteins; D012313:RNA