title: Lethal systemic capillary leak syndrome associated with severe ventilator-induced lung injury: an experimental study.
abstract: OBJECTIVE
  We report the evolution of severe ventilator-induced lung injury associated with lethal systemic capillary leak syndrome, when sheep were ventilated at a peak inspiratory pressure of 50 cm H2O, at a respiratory rate of 8 breaths.min, with an inspiratory time of 2.5 secs.
  
  
  METHODS
  A prospective laboratory animal study.
  
  
  METHODS
  Experimental animal research laboratory.
  
  
  METHODS
  Mixed breed sheep.
  
  
  METHODS
  Sheep were anesthetized, paralyzed, and mechanically ventilated.
  
  
  RESULTS
  This sheep model was characterized by a rapidly evolving massive anasarca, hemoconcentration, cardiac dysfunction, multiple system organ failure, and severe ventilator-induced lung injury. Cardiovascular changes and profound hemoconcentration developed within 6 hrs from the start of mechanical ventilation, along with a major decline in pulmonary compliance and deterioration in arterial blood gases. When total static lung compliance decreased to 0.15 mL (cm H2O)(-1) x kg(-1) (7-30 hrs), the sheep were randomized to two groups. Group I received high (recruitive) positive end-expiratory pressure (9-20 cm H2O), adjusted as needed; group II received low (supportive) positive end-expiratory pressure (2-6 cm H2O). Sheep in both groups progressively deteriorated and died with cardiocirculatory failure and multiple system organ failure within 12-24 hrs from start of treatment.
  
  
  CONCLUSIONS
  This model of lethal systemic capillary leak syndrome with multiple system organ failure differs greatly from our previous sheep model of acute ventilator-induced lung injury in which sheep were ventilated with a peak inspiratory pressure of 50 cm H2O, a respiratory rate of 4 breaths x min(-1), and an inspiratory time of 1.35 secs, without inducing capillary leak syndrome. The mere change of respiratory rate from 4 to 8 breaths x min(-1), with a near doubling of the inspiratory time to 2.5 secs, although maintaining eucapnia, resulted in lethal systemic capillary leak syndrome and multiple system organ failure with both gross and microscopic pathology of lungs greatly different from our previous model of mechanical ventilation-induced acute respiratory distress syndrome.
authors.affiliation: Pulmonary-Critical Care Medicine Branch, Section on Pulmonary and Cardiac Assist Devices, National Hearth, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Mandava
authors.forename: Theodor
authors.initials: T
authors.lastname: Kolobow
authors.forename: Giovanni
authors.initials: G
authors.lastname: Vitale
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Foti
authors.forename: Michele
authors.initials: M
authors.lastname: Aprigliano
authors.forename: Michael
authors.initials: M
authors.lastname: Jones
authors.forename: Eckhard
authors.initials: E
authors.lastname: Müller
mesh_terms: D000818:Animals; D001784:Blood Gas Analysis; D019559:Capillary Leak Syndrome / Q000175:diagnosis / Q000209:etiology* / Q000378:metabolism / Q000401:mortality; D004195:Disease Models, Animal; D018450:Disease Progression; D005652:Functional Residual Capacity; D007320:Inspiratory Capacity; D008170:Lung Compliance; D011175:Positive-Pressure Respiration / Q000009:adverse effects* / Q000379:methods*; D011446:Prospective Studies; D011897:Random Allocation; D012128:Respiratory Distress Syndrome / Q000175:diagnosis / Q000209:etiology* / Q000378:metabolism / Q000401:mortality; D015656:Respiratory Mechanics; D012756:Sheep; D016019:Survival Analysis; D013990:Tidal Volume; D013997:Time Factors; D016896:Treatment Outcome