A twisted tRNA intermediate sets the threshold for decoding.
Putting together consistent cryo-EM structure, transient kinetic and mutant tRNA suppressor data, it appears that a deformed or waggling aminoacyl-tRNA is the critical transitional structure examined by the ribosome during decoding at the A site. The unusual conformation may be required for effective proofreading of the codon-anticodon complex.
Yarus
Michael
M
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA. yarus@stripe.colorado.edu
Valle
Mikel
M
Frank
Joachim
J
D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008854:Microscopy, Electron; D009154:Mutation; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D012329:RNA, Bacterial; D012343:RNA, Transfer