Three-dimensional model of bone marrow stromal cell culture.
In a hematopoietic microenvironment in vivo, spatial organisation of hematopoiesis is possible due to the existence of a three-dimensional framework, the main part of which is formed by a branching population of stromal cells. Most of the previous in vitro studies, concerning long-term bone marrow cultures, were based on a previously prepared, flat adherent layer of stromal cells. There are only few reports concerning the three-dimensional growth pattern of the bone marrow stroma. In the present study we used a new three-dimensional model of the stromal cell culture. The framework for the cultured stromal cells was a structure of a nonliving trabecular bone (Unilab Surgibone). After a period of about four weeks the stromal cells created a spatial network which filled the intertrabecular spaces of the spongy bone.
Januszewski
Maciej
M
Orthopeadic Surgery Department of the L. Rydygier's Hospital, os. Złotej Jesieni 1, Cracow, Poland.
Dabrowski
Zbigniew
Z
Adamus
Maria
M
Niedźwiedzki
Tadeusz
T
D000818:Animals; D001853:Bone Marrow / Q000254:growth & development* / Q000648:ultrastructure*; D001857:Bone Matrix / Q000166:cytology / Q000254:growth & development / Q000648:ultrastructure; D018786:Bone Substitutes; D016025:Bone Transplantation / Q000502:physiology; D001842:Bone and Bones / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure; D002417:Cattle; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D005269:Femur / Q000166:cytology / Q000254:growth & development / Q000648:ultrastructure; D006410:Hematopoiesis / Q000502:physiology; D008297:Male; D011817:Rabbits; D017154:Stromal Cells / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods
D018786:Bone Substitutes