[Function analysis of a wheat phosphate transporter in yeast mutant].
Phosphorus is a major nutrient acquired by plants and phosphorus availability is considered one of the major growth-limiting factors for plants in many natural ecosystems. For a better understanding of the function of the wheat phosphorus transporter gene named TaPT2, we did Southern blot analysis and studied its function with complementation test in yeast mutant strain MB192. Southern blot indicated that TaPT2 gene is a low-copy member and has several different members in wheat genome. In the function complementation study, TaPT2 has a similar function as PHO84 which is the phosphate transporter in yeast Saccharomyces cerevisiae. Both TaPT2 and PHO84 are able to complement the PHO84 mutant phenotype of yeast strain MB192 which lacks the phosphate transporter activity. Therefor, this assessment presents evidence that TaPT2 gene plays an important role in Pi acquisition.
Zeng
Ya-Juan
YJ
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Ying
Jia
J
Liu
Jian-Zhong
JZ
Sun
Jian-Hua
JH
Li
Bin
B
Xiao
Hua-Shan
HS
Li
Zhen-Sheng
ZS
D015139:Blotting, Southern; D002455:Cell Division / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005816:Genetic Complementation Test; D009154:Mutation; D028061:Phosphate Transport Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
D018744:DNA, Plant; D028061:Phosphate Transport Proteins