title: Involvement of caveolin-2 in caveolar biogenesis in MDCK cells.
abstract: Caveolins have been identified as key components of caveolae, specialized cholesterol-enriched raft domains visible as small flask-shaped invaginations of the plasma membrane. In polarized MDCK cells caveolin-1 and -2 are found together on basolateral caveolae whereas the apical membrane, where only caveolin-1 is present, lacks caveolae. Expression of a caveolin mutant prevented the formation of the large caveolin-1/-2 hetero-oligomeric complexes, and led to intracellular retention of caveolin-2 and disappearance of caveolae from the basolateral membrane. Correspondingly, in MDCK cells over-expressing caveolin-2 the basolateral membrane exhibited an increased number of caveolae. These results indicate the involvement of caveolin-2 in caveolar biogenesis.
authors.affiliation: Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
authors.forename: Ulla
authors.initials: U
authors.lastname: Lahtinen
authors.forename: Masanori
authors.initials: M
authors.lastname: Honsho
authors.forename: Robert G
authors.initials: RG
authors.lastname: Parton
authors.forename: Kai
authors.initials: K
authors.lastname: Simons
authors.forename: Paul
authors.initials: P
authors.lastname: Verkade
mesh_terms: D000818:Animals; D021941:Caveolae / Q000502:physiology* / Q000648:ultrastructure; D051243:Caveolin 2; D022461:Caveolins / Q000502:physiology*; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron
chemical_list: D051243:Caveolin 2; D022461:Caveolins