title: Levitation and movement of human tumor cells using a printed circuit board device based on software-controlled dielectrophoresis.
abstract: In this study we describe an original, efficient, and innovative printed circuit board (PCB) device able to generate dielectrophoresis-based, software-controlled cages that can be moved to any place inside a microchamber. Depending on their dielectrophoretic properties, eukaryotic cells can be "entrapped" in cages and moved under software control. The main conclusion gathered from the experimental data reported is that the PCB device based on dielectrophoresis permits levitation and movement of different tumor cells at different dielectrophoresis conditions. The results presented herein are therefore the basis for experiments aimed at forced interactions or separation of eukaryotic cells using "lab-on-a-chip." In fact, because many cages can be controlled at the same time, and two or more cages can be forced to share the same or a different location, it is possible, in principle, either to bring in contact cells of a differing histotype or to separate them.
authors.affiliation: Center of Excellence on Electronic Systems (ARCES), University of Bologna, Bologna, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Altomare
authors.forename: M
authors.initials: M
authors.lastname: Borgatti
authors.forename: G
authors.initials: G
authors.lastname: Medoro
authors.forename: N
authors.initials: N
authors.lastname: Manaresi
authors.forename: M
authors.initials: M
authors.lastname: Tartagni
authors.forename: R
authors.initials: R
authors.lastname: Guerrieri
authors.forename: R
authors.initials: R
authors.lastname: Gambari
mesh_terms: D000818:Animals; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D004574:Electromagnetic Fields; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D019169:Jurkat Cells / Q000528:radiation effects; D020014:K562 Cells / Q000528:radiation effects; D007938:Leukemia; D004915:Leukemia, Erythroblastic, Acute; D008545:Melanoma; D051379:Mice; D008839:Microelectrodes; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods*; D008904:Miniaturization; D009038:Motion; D014407:Tumor Cells, Cultured / Q000528:radiation effects; D014584:User-Computer Interface