title: Fabrication of a protein film by electrospray deposition method and investigation of photochemical properties by persistent spectral hole burning.
abstract: Transparent protein film of iron-free cytochrome c (Cyt. c) was successfully manufactured by electrospray deposition (ES) method and the properties of the protein film were investigated. Excursion temperature dependence of spectral hole profiles in the photochemical hole burning for the iron-free Cyt. c in protein film and in glassy solution was investigated and compared with that of iron-free porphyrin embedded in a synthetic polymer film to clarify photochemical properties of electrospray deposited protein film. The spectral holes of iron-free porphyrin were thermally more stable in Cyt. c than in polymer, indicating compact packing of the chromophore in the protein. The ES-deposited iron-free Cyt. c in protein film showed less stable spectral hole than that in glassy solution. This difference is attributable to the electrospray deposition process of the protein involving ionization and to subsequent cross-linking of protein molecules.
authors.affiliation: Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, 113-8656, Tokyo, Japan.
authors.forename: Bumhwan
authors.initials: B
authors.lastname: Lee
authors.forename: Noriho
authors.initials: N
authors.lastname: Kamiya
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Machida
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yamagata
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Horie
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nagamune
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents / Q000494:pharmacology; D045304:Cytochromes c / Q000737:chemistry; D005898:Glass; D006736:Horses; D007501:Iron / Q000737:chemistry; D008027:Light; D009206:Myocardium / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods*; D013053:Spectrophotometry; D013696:Temperature; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D011108:Polymers; D011166:Porphyrins; D011506:Proteins; D045304:Cytochromes c; D007501:Iron