The optimization of HPLC-UV conditions for use with FTIR detection in the analysis of B vitamins.
The water-soluble vitamins thiamine (B(1)), riboflavin (B(2)), pantothenic acid (B(5)), and pyridoxine (B(6)) are separated by high-performance liquid chromatography. The mobile phase, column temperature, and flow rate are optimized so that the chromatograph can be used with a Fourier-transform infrared (FTIR) detector. Reproducibility, linearity, and detection limits are evaluated for method validation. Finally, this method is successfully transferred to liquid chromatography-FTIR with a standard mixture.
Li
Ying
Y
Department of Chemistry, University of Rhode Island, Kingston, RI, 02881, USA.
Brown
Phyllis R
PR
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010205:Pantothenic Acid / Q000032:analysis*; D011736:Pyridoxine / Q000032:analysis*; D015203:Reproducibility of Results; D012256:Riboflavin / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013831:Thiamine / Q000032:analysis*
D010205:Pantothenic Acid; D011736:Pyridoxine; D012256:Riboflavin; D013831:Thiamine