title: Tropane alkaloids in auxin-independent root cultures of Physochlaina physaloides.
abstract: Adventitious and hairy root cultures of Physochlaina physaloides were established. These roots grew well and produced high amounts of tropane alkaloids (particularly hyoscyamine and 6 beta-hydroxyhyoscyamine) in auxin-free culture medium. The effects of basal media and temperature on the growth and alkaloid production of these roots were investigated. Both root cultures produced highest amount of tropane alkaloids in B5 medium though the optimum temperature for hairy roots were lower than that for adventitious roots.
authors.affiliation: Faculty of Life Science, Toyo University, 1-1-1 Izumino, Itakura-machi, Oura-gun, Gunma, 374-0193 Japan.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Shimomura
authors.forename: Makoto
authors.initials: M
authors.lastname: Hirose
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Natori
authors.forename: Motoyoshi
authors.initials: M
authors.lastname: Satake
authors.forename: Kayo
authors.initials: K
authors.lastname: Yoshimatsu
authors.forename: Kanji
authors.initials: K
authors.lastname: Ishimaru
mesh_terms: D003470:Culture Media; D007210:Indoleacetic Acids; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D019657:Solanaceae / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature; D014326:Tropanes / Q000378:metabolism*
chemical_list: D003470:Culture Media; D007210:Indoleacetic Acids; D014326:Tropanes