title: PRISM, a generic large scale proteomic investigation strategy for mammals.
abstract: We have developed a systematic analytical approach, termed PRISM (Proteomic Investigation Strategy for Mammals), that permits routine, large scale protein expression profiling of mammalian cells and tissues. PRISM combines subcellular fractionation, multidimensional liquid chromatography-tandem mass spectrometry-based protein shotgun sequencing, and two newly developed computer algorithms, STATQUEST and GOClust, as a means to rapidly identify, annotate, and categorize thousands of expressed mammalian proteins. The application of PRISM to adult mouse lung and liver resulted in the high confidence identification of over 2,100 unique proteins including more than 100 integral membrane proteins, 400 nuclear proteins, and 500 uncharacterized proteins, the largest proteome study carried out to date on this important model organism. Automated clustering of the identified proteins into Gene Ontology annotation groups allowed for streamlined analysis of the large data set, revealing interesting and physiologically relevant patterns of tissue and organelle specificity. PRISM therefore offers an effective platform for in-depth investigation of complex mammalian proteomes.
authors.affiliation: Program in Proteomics and Bioinformatics, Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario M5G 1L6, Canada.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kislinger
authors.forename: Khaled
authors.initials: K
authors.lastname: Rahman
authors.forename: Dragan
authors.initials: D
authors.lastname: Radulovic
authors.forename: Brian
authors.initials: B
authors.lastname: Cox
authors.forename: Janet
authors.initials: J
authors.lastname: Rossant
authors.forename: Andrew
authors.initials: A
authors.lastname: Emili
mesh_terms: D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D008099:Liver / Q000502:physiology; D008168:Lung / Q000502:physiology; D008322:Mammals; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008813:Mice, Inbred ICR; D015388:Organelles / Q000502:physiology; D040901:Proteomics / Q000379:methods*
chemical_list: D008565:Membrane Proteins