title: Nutrient-gene interactions in mitochondrial function: vitamin A needs are increased in BHE/Cdb rats.
abstract: The BHE/Cdb rat has a maternally inherited mutation in the ATPase 6 mitochondrial gene that associates with impaired oxidative phosphorylation (OXPHOS) and glucose intolerance. A longevity study revealed that feeding an egg-rich (vitamin A-rich) diet delayed the onset of impaired glucose tolerance. Two experiments were conducted to test the hypothesis that BHE/Cdb rats require more dietary vitamin A than normal rats. Experiment 1 was a dose-response study examining OXPHOS in BHE/Cdb rats fed one of six levels of vitamin A. In experiment 2 BHE/Cdb and Sprague-Dawley rats were used. The rats were depleted of retinol stores, then repleted with 4 or 12 IU vitamin A/g diet. Vitamin A status was assessed in depleted, never depleted, and depleted/repleted rats. OXPHOS was optimized at 4 IU/g diet for the Sprague-Dawley rats and 12 IU/g diet for the BHE/Cdb rats. These results suggested that the criteria for vitamin intake adequacy in the BHE/Cdb rats is the optimization of mitochondrial OXPHOS. Using this criteria, we conclude that diabetes-prone BHE/Cdb rats require more dietary vitamin A than normal rats.
authors.affiliation: Department of Foods and Nutrition, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: Helen B
authors.initials: HB
authors.lastname: Everts
authors.forename: Carolyn D
authors.initials: CD
authors.lastname: Berdanier
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D005260:Female; D018149:Glucose Intolerance / Q000235:genetics / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism; D009154:Mutation; D009751:Nutritional Requirements; D010085:Oxidative Phosphorylation; D051381:Rats; D011922:Rats, Mutant Strains; D017207:Rats, Sprague-Dawley; D014801:Vitamin A / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism*
chemical_list: D014801:Vitamin A; D000251:Adenosine Triphosphatases