title: The dual activity of pyruvate kinase type M2 from chromatin extracts of neoplastic cells.
abstract: Pyruvate kinase type M(2) from Morris hepatoma 7777 tumour cell nuclei and cytosol, in contrast to types L and M(2) from nuclei and cytosol of normal rat liver, shows the histone H(1) kinase activity. Moreover, in the presence of L-cysteine and without ADP it converts 2-phosphoenolpyruvate (PEP) to pyruvate while in the presence of L-arginine or L-histidine does not. L-Cysteine markedly stimulates the activity of histone H(1) kinase transferring a phosphate group from PEP to, as results suggested, the epsilon -amino group of L-lysine of histone H(1). This, L-cysteine which is known to inhibit the activity of pyruvate kinase type M(2) from neoplastic cells transfering a phosphate from PEP to ADP, can act as a control factor champing the direction of enzymatic reaction in cancer cells.
authors.affiliation: Department of General Biochemistry, Institute of Medical Biochemistry, Collegium Medicum, Jagiellonian University, 7 Kopernika Street, 31-034 Cracow, Poland. mbignaca@cyf-kr.edu.pl
authors.forename: Jan
authors.initials: J
authors.lastname: Ignacak
authors.forename: Maria Barbara
authors.initials: MB
authors.lastname: Stachurska
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002458:Cell Fractionation; D002843:Chromatin / Q000302:isolation & purification / Q000378:metabolism; D003545:Cysteine / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006639:Histidine / Q000494:pharmacology; D007527:Isoenzymes / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D010728:Phosphoenolpyruvate / Q000494:pharmacology; D011770:Pyruvate Kinase / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011915:Rats, Inbred BUF
chemical_list: D002843:Chromatin; D004791:Enzyme Inhibitors; D007527:Isoenzymes; D006639:Histidine; D010728:Phosphoenolpyruvate; D000255:Adenosine Triphosphate; D001120:Arginine; D011770:Pyruvate Kinase; D003545:Cysteine