title: Jatrophane diterpenes from the latex of Euphorbia obtusifolia with inhibitory activity on the mammalian mitochondrial respiratory chain.
abstract: Seven diterpenes isolated of the latex of the Euphorbia obtusifolia were evaluated as inhibitors of the NADH oxidase activity in submitochondrial particles from bovine heart. Compound 2, 2,3,5,7,8,9,15-heptahydroxyjatropha-6(17),11-diene-14-one 8,9-diacetate 7-isobutyrate 2,3-bis(2-methylbutyrate), was the most potent inhibitor with an inhibitory concentration (IC 50 ) value of 5.1 +/- 0.2 microM. In the present study, some structure-activity trends are suggested for the inhibitory activity of these natural products on the mammalian mitochondrial respiratory chain.
authors.forename: Liliana
authors.initials: L
authors.lastname: Betancur-Galvis
authors.forename: Javier
authors.initials: J
authors.lastname: Checa
authors.forename: J Alberto
authors.initials: JA
authors.lastname: Marco
authors.forename: Ernesto
authors.initials: E
authors.lastname: Estornell
mesh_terms: D000818:Animals; D002417:Cattle; D004224:Diterpenes / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D028481:Euphorbia; D006801:Humans; D020128:Inhibitory Concentration 50; D007840:Latex / Q000737:chemistry; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000201:enzymology; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D008517:Phytotherapy; D013329:Structure-Activity Relationship
chemical_list: D004224:Diterpenes; D007840:Latex; D009097:Multienzyme Complexes; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases