Afa/Dr diffusely adhering Escherichia coli infection in T84 cell monolayers induces increased neutrophil transepithelial migration, which in turn promotes cytokine-dependent upregulation of decay-accelerating factor (CD55), the receptor for Afa/Dr adhesins.
Ulcerative colitis and Crohn's disease are inflammatory bowel diseases thought to involve strains of Escherichia coli. We report here that two wild-type Afa/Dr diffusely adhering E. coli (DAEC) strains, C1845 and IH11128, which harbor the fimbrial F1845 adhesin and the Dr hemagglutinin, respectively, and the E. coli laboratory strain HB101, transformed with the pSSS1 plasmid to produce Afa/Dr F1845 adhesin, all induced interleukin-8 (IL-8) production and transepithelial migration of polymorphonuclear leukocytes (PMNL) in polarized monolayers of the human intestinal cell line T84 grown on semipermeable filters. We observed that after PMNL migration, expression of decay-accelerating factor (DAF, or CD55), the brush border-associated receptor for Afa/Dr adhesins, was strongly enhanced, increasing the adhesion of Afa/Dr DAEC bacteria. When examining the mechanism by which DAF expression was enhanced, we observed that the PMNL transepithelial migration induced epithelial synthesis of tumor necrosis factor alpha and IL-1beta, which in turn promoted the upregulation of DAF.
Bétis
Fréderic
F
Unité INSERM 36, IFR 50, Faculté de Médecine, avenue de Valombrose, 06107 Nice Cédex 02, France.
Brest
Patrick
P
Hofman
Véronique
V
Guignot
Julie
J
Kansau
Imad
I
Rossi
Bernard
B
Servin
Alain
A
Hofman
Paul
P
D018830:Adhesins, Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D001422:Bacterial Adhesion; D018958:CD55 Antigens / Q000378:metabolism*; D016764:Cell Polarity; D016207:Cytokines / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006388:Hemagglutinins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008854:Microscopy, Electron; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000276:immunology; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D018830:Adhesins, Escherichia coli; D018958:CD55 Antigens; D016207:Cytokines; C465564:Dr adhesin, E coli; D029968:Escherichia coli Proteins; D006388:Hemagglutinins