An interface chip connection between capillary electrophoresis and thermal lens microscope.
A thermal lens microscope (TLM) detection of capillary electrophoresis (CE) utilizing microchip technology was developed. Fused-silica capillaries with an inner diameter of 50 microm were directly connected to a microchannel in a microchip. The detection limit by TLM was estimated as 2.8 x 10(-7) absorbance by measuring pure water. The detection limit of derivatized amino acids determined by CE-TLM was estimated as 2.4 x 10(-8) M, which was 100 times lower than that of conventional absorbance detection.
Uchiyama
Kenji
K
Integrated Chemistry Project, Kanagawa Academy of Science and Technology, Kawasaki, Japan.
Hibara
Akihide
A
Sato
Kiichi
K
Hisamoto
Hideaki
H
Tokeshi
Manabu
M
Kitamori
Takehiko
T
D000596:Amino Acids / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D007202:Indicators and Reagents; D008832:Microchemistry / Q000295:instrumentation; D008853:Microscopy / Q000295:instrumentation*; D012822:Silicon Dioxide; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives*
D000596:Amino Acids; D007202:Indicators and Reagents; C010572:4-N,N-dimethylaminoazobenzene-4'-sulfonyl chloride; D012822:Silicon Dioxide; D004124:p-Dimethylaminoazobenzene