Inhibition of mutagenicity of food-derived heterocyclic amines by sulphoraphene--an isothiocyanate isolated from radish.
The naturally derived isothiocyanate, sulphoraphene [4-isothiocyanato-(1R)-(methylsulphinyl)-1-(E)-butene], isolated from seeds of radish ( Raphanus sativus L., Cruciferae) was investigated for its antigenotoxic effects against a battery of cooked food mutagens (heterocyclic amines) in the Ames Salmonella/reversion assay using Salmonella typhimurium TA98 (frame-shift mutation sensitive) and TA100 (base -pair mutation sensitive) bacterial strains in the presence of Aroclor 1254 induced rat liver S9. Results of the present in vitro anti-mutagenicity studies using the base-pair mutation sensitive strain TA100, strongly suggest that sulphoraphene is a potent inhibitor of the S9-mediated mutagenicity of all the tested heterocyclic amines (60 - 75 % inhibition at a dose of 500 nmol/plate).
Shishu
Singla
A K
AK
Kaur
I P
IP
D000588:Amines / Q000633:toxicity*; D000818:Animals; D020111:Chlorodiphenyl (54% Chlorine); D006571:Heterocyclic Compounds / Q000633:toxicity; D017879:Isothiocyanates / Q000633:toxicity*; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D008517:Phytotherapy; D031224:Raphanus; D051381:Rats; D017207:Rats, Sprague-Dawley; D012486:Salmonella typhimurium / Q000187:drug effects; D012639:Seeds
D000588:Amines; D006571:Heterocyclic Compounds; D017879:Isothiocyanates; D009153:Mutagens; D020111:Chlorodiphenyl (54% Chlorine); C473643:sulphoraphene