[Evaluation of the usefulness of three collections of bacteriophages for typing methicillin-resistant Staphylococcus aureus, isolated in Moscow hospitals].
The typing of S. aureus methicillin-resistant strains, isolated in different hospitals of Moscow; was carried out with the use of three collections of phages: the International Set of Phages; the set of phages of the International Center of S. aureus phage typing in London (L); and the experimental collection of phages of the Gamaleya Institute of Epidemiology and Microbiology in Moscow (M). In this study made with the use of both the phages of the International Diagnostic Set and phages L in the standard typing dose of 1 TP about 6% of the cultures under study proved to be sensitive. When the typing dose was increased to 100 TP the phages of the international diagnostic set lyzed 75.5% of the cultures. The typed strains were found to belong to phage types 77 (71.7%), 77/84/85 (19.6%) and 94/96 (6.5%). At a concentration of 100 TP phages L lyzed 83.7% of the cultures, but the dominating phage types could not be determined due to a great variety of phage markers. In contrast to the two preceding collections, the third phage collection M was composed in such a way that in the study of the investigated culture the specificity of its restriction modification was primarily evaluated and only then the presence of antiphage immunity was determined. This latter collection was used in the evaluation of 93.1% of the cultures. By the specificity of their restriction specification system the majority of them were classified with two new groups, heretofore not described. Only this collection M made it possible to differentiate epidemic and sporadic strains and to evaluate the epidemic situation in all 6 hospitals.
Dmitrienko
O A
OA
Gamaleya Research Institute of Epidemiology and Microbiology, Federal State Unitary Enterprise State Research Center on Antibiotics, Botkin City Clinical Hospital, Pirogov City Clinical Hospital No. 1, Vishnevsky Institute of Surgery, Moscow, Russia.
Sidorenko
S V
SV
Zhukhovitskiĭ
V G
VG
Terekhova
R V
RV
Karabak
V I
VI
Tarasevich
N N
NN
Vasil'eva
E I
EI
Prokhorov
V Ia
VIa
D001434:Bacteriophage Typing / Q000379:methods; D006801:Humans; D016106:Methicillin Resistance; D009031:Moscow; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification