Expression of the LIM proteins paxillin and Hic-5 in human tissues.
The LIM domain is a protein-protein interaction motif critically involved in a variety of fundamental biological processes, including cytoskeletal organization, cell lineage specification, and organ development. In this study we examined the expression of the LIM proteins paxillin and Hic-5 in adult human tissues by immunohistochemistry and immunoblotting. Paxillin expression was widespread and observed both in non-muscle and muscle tissues. Of the latter, paxillin was mainly expressed in multinuclear striated muscle. In contrast, Hic-5 showed restricted expression and was expressed in muscle tissues, mainly in mononuclear smooth muscle. Taken together with previous findings, it appears likely that the counterbalance between paxillin and Hic-5 may be deeply involved in muscle differentiation.
Yuminamochi
Tsutomu
T
Department of Clinical Laboratory, Yamanashi Medical University, Japan.
Yatomi
Yutaka
Y
Osada
Makoto
M
Ohmori
Tsukasa
T
Ishii
Yoshio
Y
Nakazawa
Kumiko
K
Hosogaya
Shigemi
S
Ozaki
Yukio
Y
D000328:Adult; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D018482:Muscle, Skeletal / Q000378:metabolism; D009130:Muscle, Smooth / Q000378:metabolism; D009928:Organ Specificity; D051419:Paxillin; D010750:Phosphoproteins / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; C496061:PXN protein, human; D051419:Paxillin; D010750:Phosphoproteins; D016212:Transforming Growth Factor beta