Lactobacillus ingluviei sp. nov., isolated from the intestinal tract of pigeons.
Lactic acid bacteria were isolated from the crop and intestines of pigeons. One group of strains, showing similar genomic patterns after screening with tRNA intergenic spacer PCR, could not be identified to the species level. Sequencing of the 16S rRNA gene of one representative strain revealed about 96% similarity to sequences from Lactobacillus fermentum and Lactobacillus mucosae. Determination of the DNA base composition, DNA-DNA hybridization experiments, SDS-PAGE of whole-cell proteins and biochemical testing confirmed that the seven strains studied constitute a single novel Lactobacillus species, for which the name Lactobacillus ingluviei sp. nov. is proposed. The type strain is strain KR3T (=LMG 20380T =CCUG 45722T).
Baele
Margo
M
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Vancanneyt
Marc
M
BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Devriese
Luc A
LA
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Lefebvre
Karen
K
BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Swings
Jean
J
BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Haesebrouck
Freddy
F
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
D000818:Animals; D001483:Base Sequence; D001717:Birds / Q000382:microbiology; D003426:Crop, Avian / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005838:Genotype; D007422:Intestines / Q000382:microbiology; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S