title: Homo- and hetero-complexes of exchangeable apolipoproteins in solution and in lipid-bound form.
abstract: The self-association state of human plasma apolipoprotein E (apoE) in solution and in complexes with dimyristoylphosphatidylcholine (DMPC) varying in stoichiometry was studied in sub-micromolar concentration range by gel filtration, fluorescence anisotropy, fluorescence quenching and energy transfer measurements with apolipoprotein labeled with lysine-specific fluorescent dyes. Together, these results confirm the equilibrium scheme for various apoE structures in solution: oligomer (in aged preparations) <==> 'closed' tetramer <==> 'open' tetramer ('molten globule' state) <==> native or partially denatured monomer <==> fully denatured monomer. Within DMPC:apoE discoidal complex (125:1) the apolipoprotein association state seems to be intermediate between that in solution and in larger vesicular complex (1000:1); for both complexes, the degree of exposure of fluorescein chromophores into water phase decreased. Hetero-associates of apoA-I and apoC-III-1 in solution and in the complexes with DMPC appear to behave similarly to apoE. When extrapolated to native HDL particles, 'molten globule' state seems to be a structure responsible for the interaction of exchangeable apolipoproteins with phospholipid. For a first time, the location of various apolipoprotein molecules on disc periphery was confirmed. The lysine residue(s) seems to locate closely to reacting residue(s) within apolipoprotein molecules in associates, however, with different package constraints for discoidal versus vesicular complexes with phospholipid.
authors.affiliation: National Research Center for Preventive Medicine, 10, Petroverigsky street, 101953 Moscow, Russia. dergunov@img.ras.ru
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Dergunov
authors.forename: Yulia Y
authors.initials: YY
authors.lastname: Vorotnikova
authors.forename: Sophie
authors.initials: S
authors.lastname: Visvikis
authors.forename: Gerard
authors.initials: G
authors.lastname: Siest
mesh_terms: D001053:Apolipoproteins / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission
chemical_list: D001053:Apolipoproteins; D008055:Lipids