title: Fatty acid analysis of Stenotrophomonas maltophilia clinical strains showing different susceptibility to antibiotics at 30 and 37 degrees C.
abstract: Isolates of Stenotrophomonas maltophilia species display the feature "temperature-dependent susceptibility" (TDS) to antibiotics. Both 30TDS strains (at least 4 times lower value of minimum inhibitory concentration (MIC) of an antibiotic at 30 than at 37 degrees C) and 37TDS strains (at least 4 times lower value of MIC at 37 than at 30 degrees C) were described. Changes in the distribution of saturated and unsaturated fatty acids (FA) at 30 and 37 degrees C were considered as one of possible causes of the TDS phenomenon. Gas chromatography was used to determine the distribution of individual FA in five 37TDS strains of S. maltophilia (Group I); in five strains with MIC values unaffected by the cultivation temperature (Group II) and in six 30TDS (four strains) or 30/37TDS (two strains) isolates (Group III). At identical temperatures, no statistically significant differences in the distribution of major FA (iso-15:0, anteiso-15:0, 16:0 and 16:1) were registered between individual groups. Statistically significant (p < 0.05) differences between groups were found in minor FA only (iso-16:0, iso-17:0 and iso-17:1). Distribution changes of cellular FA at 30 and 37 degrees C can be considered to play only a minor role in the formation of the TDS phenomenon.
authors.affiliation: Institutes of Microbiology and Pathological Physiology, Faculty of Medicine, Palacký University, 775 15 Olomouc, Czechia. hejnar@fnol.cz
authors.forename: P
authors.initials: P
authors.lastname: Hejnar
authors.forename: Z
authors.initials: Z
authors.lastname: Chmela
authors.forename: M
authors.initials: M
authors.lastname: Rypka
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D006801:Humans; D008826:Microbial Sensitivity Tests; D020615:Stenotrophomonas maltophilia / Q000187:drug effects* / Q000378:metabolism*; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D005230:Fatty Acids, Nonesterified