Recovery of feline calicivirus infectious particles and genome from water: comparison of two concentration techniques.
The aim of this work was to determine the recovery rate of feline calicivirus (FCV-F9) infectious particles and genome from water after a concentration step using either adsorption elution on glass wool or filtration through an electropositive membrane. The results showed that the membrane filtration technique allowed a 75% recovery rate of FCV-F9 infectious particles while the yield was only 5.3% for FCV-F9 genome. Using the glass wool adsorption-elution technique, the recovery rate of FCV-F9 infectious particles was 0.5% while the yield was 102.5% with Poliovirus 1.
Gassilloud
B
B
LCPME UMR-UHP-CNRS 7564, Faculté de Pharmacie, 54003 Nancy, France.
Duval
M
M
Schwartzbrod
L
L
Gantzer
C
C
D000327:Adsorption; D017927:Calicivirus, Feline / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D004279:DNA, Viral / Q000032:analysis*; D004784:Environmental Monitoring; D005374:Filtration; D008567:Membranes, Artificial; D014871:Water Microbiology; D014881:Water Supply
D004279:DNA, Viral; D008567:Membranes, Artificial