title: Resolving the ionotropic receptor kinetics and modulation in the time scale of synaptic transmission.
abstract: Synaptic transmission plays a crucial role in signal transduction in the adult central nervous system. It is known that synaptic transmission can be modulated by physiological and pathological processes and a number of factors including metal ions, pH, drugs, etc. The patch-clamp technique allows to measure postsynaptic currents, but the mechanism of these currents modulation remains unclear. The estimated value of neurotransmitter transient indicates that this time course is very short and the activation of postsynaptic receptors is extremely non-equilibrient. The ultrafast perfusion system makes it possible to mimic synaptic conditions and, additionally, the agonist concentration can be controlled, which is very important for pharmacokinetic studies. In the present paper, examples of pharmacological modulation of mIPSC kinetics and currents evoked by ultrafast agonist application are presented.
authors.affiliation: Department of Biophysics, Wrocław Medical University, Chałubińskiego 10, Poland. maja@biofiz.am.wroc.pl
authors.forename: Maria
authors.initials: M
authors.lastname: Pytel
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Mercik
authors.forename: Jerzy W
authors.initials: JW
authors.lastname: Mozrzymas
mesh_terms: D002460:Cell Line; D002746:Chlorpromazine / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D015640:Ion Channel Gating; D007700:Kinetics; D018408:Patch-Clamp Techniques; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*
chemical_list: D011963:Receptors, GABA-A; D011994:Recombinant Proteins; D002746:Chlorpromazine