title: Effects of hyperthermia on rat hippocampal pyramidal cell apoptosis in vitro.
abstract: OBJECTIVE
  To investigate the changes in the morphology and biochemistry of neonatal rat hippocampal pyramidal cells (HPCs)apoptosis in high-temperature environment.
  
  
  METHODS
  In vitro culture of HPCs was established and then subjected to environmental stimulation with high temperatures (37, 40, and 42 degrees Celsius respectively) for 1 h. All the cells were collected at different time points after the treatment and examined by flow cytometery, fluorescence staining, agarose gel electrophoresis and in situ cell apoptosis detection kit.
  
  
  RESULTS
  The apoptotic rate and the number of apoptotic bodies increased as the temperature was elevated. DNA ladder was presented on agarose gel electrophoresis, and the germination of the apoptotic body was observed by fluorescence microscope.
  
  
  CONCLUSIONS
  Hyperthermia may induce the apoptosis in HPCs in vitro, and the extend of apoptosis and apoptotic behavior may vary with the temperature and treatment duration.
authors.affiliation: Department of High-temperature Medicine, First Military Medical University, Guangzhou 510515, China.
authors.forename: Guang-zZhong
authors.initials: GZ
authors.lastname: Chen
authors.forename: Bing-dDe
authors.initials: BD
authors.lastname: Luo
authors.forename: Hong-qQin
authors.initials: HQ
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Zou
authors.forename: Wei-rRen
authors.initials: WR
authors.lastname: Wan
authors.forename: Xiao-hHong
authors.initials: XH
authors.lastname: Shen
mesh_terms: D000818:Animals; D017209:Apoptosis; D001831:Body Temperature; D002478:Cells, Cultured; D005260:Female; D006624:Hippocampus / Q000166:cytology*; D006979:Hyperthermia, Induced / Q000009:adverse effects*; D008297:Male; D017966:Pyramidal Cells / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley