title: Acidification modulates the traffic of Trypanosoma cruzi trypomastigotes in Vero cells harbouring Coxiella burnetii vacuoles.
abstract: We studied the fate of different Trypanosoma cruzi trypomastigote forms after they invade Vero cells persistently colonised with Coxiella burnetii. When the invasion step was examined we found that persistent C. burnetii infection per se reduced only tissue-culture trypomastigote invasion, whereas raising vacuolar pH with Bafilomycin A1 and related drugs, increased invasion of both metacyclic and tissue-culture trypomastigotes when compared with control Vero cells. Kinetic studies of trypomastigote transfer indicated that metacyclic trypomastigotes parasitophorous vacuoles are more efficiently fused to C. burnetii vacuoles. The higher tissue-culture trypomastigote hemolysin and transialidase activities appear to facilitate their faster escape from the parasitophorous vacuole. Sialic acid deficient Lec-2 cells facilitate the escape of both forms. Endosomal-lysosomal sequential labelling with EEA1, LAMP-1, and Rab7 of the parasitophorous vacuoles formed during the entry of each infective form revealed that the phagosome maturation processes are also distinct. Measurements of C. burnetii vacuolar pH disclosed a marked preference for trypomastigote fusion with more acidic rickettsia vacuoles. Our results thus suggest that intravacuolar pH modulates the traffic of trypomastigote parasitophorous vacuoles in these doubly infected cells.
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo--UNIFESP, Escola Paulista de Medicina, Rua Botucatu, 862 6th floor, 04023-062, São Paulo, SP, Brazil.
authors.forename: Walter K
authors.initials: WK
authors.lastname: Andreoli
authors.forename: Renato A
authors.initials: RA
authors.lastname: Mortara
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000150:complications* / Q000635:transmission; D002522:Chlorocebus aethiops; D016997:Coxiella burnetii; D018450:Disease Progression; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal; D011778:Q Fever / Q000150:complications*; D014349:Trypanosoma cruzi; D014617:Vacuoles / Q000378:metabolism; D014709:Vero Cells / Q000382:microbiology* / Q000469:parasitology / Q000648:ultrastructure