Salmochelins, siderophores of Salmonella enterica and uropathogenic Escherichia coli strains, are recognized by the outer membrane receptor IroN.
Members of a family of catecholate siderophores, called salmochelins, were isolated by reversed-phase HPLC from Salmonella enterica serotype Typhimurium and structurally characterized by Fourier transform ion cyclotron resonance-MSMS and GC-MS. The tentative structure of salmochelin 1 contained two 2,3- dihydroxybenzoylserine moieties bridged by a glucose residue, bound to the serine hydroxyl group of one moiety and the carboxylate of the second moiety. Salmochelin 2 contained in addition a second glucose residue linked to a third 2,3-dihydroxybenzoylserine moiety. Salmochelins were not produced by an iroBC mutant, which indicated that the IroB protein might be responsible for the glucosyl transfer predicted by sequence similarities to known glycosyltransferases. Uptake experiments with radiolabeled (55)Fe-salmochelin and growth promotion tests with salmochelins showed that the IroN outer membrane receptor, encoded in the iroA locus of S. enterica and uropathogenic Escherichia coli strains, was the main receptor for ferric salmochelin transport.
Hantke
K
K
MikrobiologieMembranphysiologie, MikrobiologieBiotechnologie, Institut für Organische Chemie, Universität Tübingen, Auf der Morgenstelle 28, Tübingen, Germany. hantke@uni-tuebingen.de
Nicholson
G
G
Rabsch
W
W
Winkelmann
G
G
D002462:Cell Membrane / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000302:isolation & purification / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D019779:Salmonella enterica / Q000378:metabolism*; D017262:Siderophores / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
D029968:Escherichia coli Proteins; C469922:IroN protein, E coli; D011956:Receptors, Cell Surface; D017262:Siderophores; D007501:Iron