Sterol composition of gonad, muscle and digestive gland of Pecten maximus from Málaga (South Spain).
Sterol composition and content and their seasonal variations over 18 months were investigated in adductor muscle, digestive gland and gonads of Pecten maximus. Sterols were isolated by Silicagel 60 thin layer chromatography and identified by gas chromatography/mass spectrometry. Eleven sterols were identified, with cholesterol, brassicasterol, 24-methylenecholesterol and 22-trans-dehydrocholesterol being the principal components. The same sterols were found in all three tissues independent of season. The relative amounts of each sterol present in each tissue differed. Total sterol levels in gonad and muscle were higher than in digestive gland. Statistically significant differences (P<0.05) were found between the concentrations of each of the sterols isolated from the gonad or muscle and digestive gland. The seasonal variations in the sterol content of the gonad seem be related to the reproductive cycle, while the sterol content of the digestive gland appears to be linked to diet, mainly diatoms or dinoflagellates. The muscle sterol content showed minor changes throughout the year.
Pazos
A J
AJ
Departamento de Bioquímica y Biología Molecular, Instituto de Acuicultura, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Silva
A
A
Vázquez
V
V
Pérez-Parallé
M L
ML
Román
G
G
Sánchez
J L
JL
Abad
M
M
D000818:Animals; D002775:Cholestadienols / Q000032:analysis; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000032:analysis; D002855:Chromatography, Thin Layer; D003684:Dehydrocholesterols / Q000032:analysis; D004064:Digestive System / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006066:Gonads / Q000737:chemistry; D007536:Isomerism; D008974:Mollusca / Q000033:anatomy & histology / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000737:chemistry; D010840:Phytosterols; D012621:Seasons; D013030:Spain; D013261:Sterols / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
D002775:Cholestadienols; D003684:Dehydrocholesterols; D010840:Phytosterols; D013261:Sterols; C003532:brassicasterol; C032843:5,22-cholestadien-3beta-ol; D002784:Cholesterol; C002537:24-methylenecholesterol