Developmental responses of two substrains of in vitro fertilized C57BL/6J mouse embryos to oxygen and amino acids.
The developmental response of in vitro fertilized embryos to oxygen and amino acids were compared between in-house bred C57BL/6JNrs (Nrs) and commercially available C57BL/6JSlc (Slc) mice. Under 20% oxygen, the percentage of embryos that developed to blastocysts and expanded blastocysts, and nuclear numbers were lower in Slc embryos than in Nrs embryos. Moreover, the nuclear number did not increase in Slc embryos during 72-96 h culture. Effects of amino acids were beneficial on development of Slc embryos under 20% oxygen, but inhibitory on blastocoel formation at 78 h under 5% oxygen. On the other hand effects of amino acids on Nrs embryos were observed in nuclear number at 72 and 78 h culture under 5% oxygen. Because the present study showed differences in sensitivity to culture conditions between the C57BL/6J substrains, care must be taken in embryo manipulation of this inbred strain in studies of embryo development or other studies.
Kito
Seiji
S
Laboratory Animal Development and Management Section, National Institute of Radiological Sciences, 4-9-1 Anagawa, Chiba City, 263-8555, Japan.
Noguchi
Yoshiko
Y
Ohta
Yuki
Y
D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D002478:Cells, Cultured; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000494:pharmacology*; D013997:Time Factors
D000596:Amino Acids; D010100:Oxygen