GABAA receptor epsilon subunit expression in identified peptidergic neurons of the rat hypothalamus.
Dual-labeling immunohistochemical or in situ hybridization studies for the recently cloned epsilon-subunit and several neuropeptides were performed in the rat hypothalamus. We revealed an extensive co-expression (>90%) with hypocretin (Hcrt), oxytocin (OT), the gonadotropin-releasing hormone (GnRH), and the melanin-concentrating hormone (MCH) peptides, whereas occasional co-expression (<10%) with cocaine-amphetamine-regulated transcript (CART) was found. Our results suggest that novel GABA(A) receptor subtypes comprising epsilon-subunit are important for metabolic and neuroendocrine functions.
Moragues
Nathalie
N
Laboratoire de Neurophysiologie CNRS-UMR 5543, Université Victor Segalen Bordeaux 2, 146 rue Léo-Saignat, France.
Ciofi
Philippe
P
Lafon
Pierrette
P
Tramu
Gérard
G
Garret
Maurice
M
D000818:Animals; D007031:Hypothalamus / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis*; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000032:analysis / Q000378:metabolism; D021122:Protein Subunits / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000032:analysis / Q000096:biosynthesis*
C546278:Gabre protein, rat; D009419:Nerve Tissue Proteins; D010455:Peptides; D021122:Protein Subunits; D011963:Receptors, GABA-A