Molecular cytogenetic parameters in Ewing sarcoma.
To evaluate possible genomic instability and possible random aneuploidy, we applied comparative genomic hybridization and fluorescence in situ techniques, and evaluated telomerase activity in 16 cases of Ewing sarcoma (EWS) and compared the results to 7 controls. Common secondary aberrations (gains of chromosomes 8 and 12) were found in the study group. There was a direct correlation between the detection of random aneuploidy and development of tumor relapse (P = 0.0047). Other detectable abnormal parameters (secondary) and high telomerase activity were also more common among the cases with relapse but did not reach a statistical significance (probably because of the small sample size). In EWS, the detection of random aneuploidy seems to be a sensitive parameter in the prediction of tumor relapse.
Amiel
A
A
Genetic Institute, Sapir Medical Center, Kfar-Saba, Israel. amielaliza@clalit.org.il
Ohali
A
A
Fejgin
M
M
Sardos-Albertini
F
F
Bouaron
N
N
Cohen
I J
IJ
Yaniv
I
I
Zaizov
R
R
Avigad
S
S
D000293:Adolescent; D000328:Adult; D000782:Aneuploidy; D001859:Bone Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002648:Child; D002877:Chromosomes, Human / Q000648:ultrastructure; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015993:Life Tables; D008297:Male; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000032:analysis; D009364:Neoplasm Recurrence, Local; D009693:Nucleic Acid Hybridization; D012512:Sarcoma, Ewing / Q000201:enzymology / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D019098:Telomerase / Q000032:analysis
D009363:Neoplasm Proteins; D019098:Telomerase