title: Purification by affinity chromatography of H1o RNA-binding proteins from rat brain.
abstract: Post-transcriptional regulation of mRNA metabolism is involved in processes as different as cell fate specification in development and cell response to a large variety of environmental cues. Regulation of all steps of RNA metabolism depends on RNA-binding proteins (RBPs). By using a T1 RNase-protection assay, we previously identified three H1 degrees RNA-binding factors (p40, p70 and p110), highly expressed in the rat brain. Here we report enrichment of these factors from brain extracts, obtained by affinity chromato-graphy of biotinylated H1 degrees RNA-protein complexes on streptavidin-conjugated paramagnetic particles. The purified proteins maintain RNA-binding ability and preference for histone messages.
authors.affiliation: Dipartimento di Biologia Cellulare e dello Sviluppo Alberto Monroy, University of Palermo, Palermo, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Scaturrok
authors.forename: Anna
authors.initials: A
authors.lastname: Sala
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cutrona
authors.forename: Lavinia
authors.initials: L
authors.lastname: Raimondi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cannino
authors.forename: Simona
authors.initials: S
authors.lastname: Fontana
authors.forename: Ida
authors.initials: I
authors.lastname: Pucci-Minafra
authors.forename: Italia
authors.initials: I
authors.lastname: Di Liegro
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002846:Chromatography, Affinity; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D012313:RNA / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D016601:RNA-Binding Proteins; D012313:RNA