title: Specific determination of bromate in bread by ion chromatography with ICP-MS.
abstract: A sensitive method for detecting bromate in bread by ion chromatography with inductively-coupled plasma mass spectrometry (IC/ICP-MS) was developed. Bromate was extracted from bread with water. The clean-up procedure included a 0.2 micron filter, a C18 cartridge for defatting, a silver cartridge to remove halogen anions, a centrifugal ultrafiltration unit to remove proteins, and a cation-exchange cartridge to remove silver ions. A 500 microL sample solution was applied to IC/ICP-MS. The detection limit and the quantitation limit of bromate in the solution were 0.3 ng/mL and 1.0 ng/mL, expressed as HBrO3, respectively, which corresponded to 2 ng/g and 5 ng/g, respectively, in bread. Recovery of bromate was about 90%, and the CV was about 2%. Based on the detection limit in solution and recovery from bread, the detection limit of bromate in bread was estimated to be 2 ng/g.
authors.affiliation: National Institute of Health Sciences: 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Akiyama
authors.forename: Michiko
authors.initials: M
authors.lastname: Yamanaka
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Date
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kubota
authors.forename: Megumi Hamano
authors.initials: MH
authors.lastname: Nagaoka
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kawasaki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Chikako
authors.initials: C
authors.lastname: Yomota
authors.forename: Tamio
authors.initials: T
authors.lastname: Maitani
mesh_terms: D001939:Bread / Q000032:analysis*; D001959:Bromates / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D001959:Bromates