Visual detection of AMP and real-time monitoring of cyclic nucleotide phosphodiesterase (PDE) activity in neutral aqueous solution. Chemosensor-coupled assay of PDE and PDE inhibitors.
A phosphate chemosensor ensemble, [Zn(H-bpmp)(pyrocatechol violet)](+) is useful for a real-time assay of phosphodiesterases (PDEs) in a neutral aqueous solution. In addition, a simple and convenient screen and assay procedures for inhibitors of PDEs have been demonstrated using IBMX, a nonselective PDE inhibitor as an example.
Han
Min Su
MS
Center for Integrated Molecular Systems and Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology, San 31 Hyojadong, Pohang 790-784, South Korea.
Kim
Dong H
DH
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000378:metabolism*; D002151:Calorimetry; D007202:Indicators and Reagents; D007700:Kinetics; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
D007202:Indicators and Reagents; D010726:Phosphodiesterase Inhibitors; D012996:Solutions; D000249:Adenosine Monophosphate; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D015056:1-Methyl-3-isobutylxanthine