Studies with black tea and its constituents on leukemic cells and cell lines.
The anticancer effect of black tea (BT) and its polyphenols theaflavin (TF) and thearubigin (TR) has been evaluated on U-937 cell line, a myeloid leukemic cell line and on leukemic cells isolated from peripheral blood of chronic myeloid leukemia (CML) patients. In both types of cells, cell growth inhibition was observed 24 hrs after treatment with BT, TF and TR. MTT assay showed growth inhibition of metabolically active cells and inhibition of DNA synthesis was observed by 3H-Thymidine incorporation after treatment with the compounds. In all cases TF and TR were more effective than BT, suggesting that these are possibly the active components in BT responsible for its antileukemic activity. Superoxide dismutase (SOD), a free radical scavenger, was found to be increased by TF, whereas BT and TR lowered the level in comparison to the control. The present study is the first report of antileukemic effect of BT and its polyphenols.
Das
M
M
Indian Institute of Chemical Biology, Kolkata, India.
Chaudhuri
T
T
Goswami
S K
SK
Murmu
N
N
Gomes
A
A
Mitra
S
S
Besra
S E
SE
Sur
P
P
Vedasiromoni
J R
JR
D016588:Anticarcinogenic Agents / Q000633:toxicity*; D044946:Biflavonoids; D002392:Catechin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D007951:Leukemia, Myeloid; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D008517:Phytotherapy; D010936:Plant Extracts / Q000633:toxicity*; D059808:Polyphenols; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D013662:Tea / Q000633:toxicity; D014407:Tumor Cells, Cultured
D016588:Anticarcinogenic Agents; D044946:Biflavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D013662:Tea; C086701:thearubigin; C056068:theaflavin; D002392:Catechin; D013482:Superoxide Dismutase