Protection of epidermal cells against UVB injury by the antioxidant selenium-containing single-chain Fv catalytic antibody.
The antioxidant effect of selenium-containing single-chain Fv catalytic antibody (Se-scFv2F3), a new mimic of glutathione peroxidase, was confirmed using a model system in which cultured rat skin epidermal cells were injured by ultraviolet B (UVB). The cell damage was characterized in terms of lipid peroxidation of the cells, cell viability, and cell membrane integrity. The injury effects of UVB and protection effects of Se-scFv2F3 on the cells were studied using the model system. UVB can damage the cells severely. Upon precultivation of the cells with 0.4U/ml Se-scFv2F3, however, the damage was significantly reduced as shown by the increase in cell viability, the decrease in the malondialdehyde and hydrogen peroxide levels, and the normalization of lactate dehydrogenase activity. In addition, a novel finding that Se-scFv2F3 can stimulate cultured epidermal cells to proliferate under certain conditions was observed.
Sun
Ye
Y
Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, 125 Jiefang Road, Changchun 130023, People's Republic of China.
Mu
Ying
Y
Li
Weijia
W
Lv
Shaowu
S
Jiang
Zhihua
Z
Zhang
Kun
K
Zheng
Keyan
K
Lin
Feng
F
Yan
Ganglin
G
Luo
Guimin
G
Liu
Junqiu
J
Shen
Jiacong
J
D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000737:chemistry* / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D002384:Catalysis; D002455:Cell Division; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D000078404:Epidermal Cells; D004817:Epidermis / Q000528:radiation effects*; D005609:Free Radicals; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000737:chemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D050356:Lipid Metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D012643:Selenium / Q000494:pharmacology*; D014466:Ultraviolet Rays
D000906:Antibodies; D000975:Antioxidants; D005609:Free Radicals; D007135:Immunoglobulin Variable Region; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D005979:Glutathione Peroxidase; D012643:Selenium