title: Calcium/calmodulin-dependent protein kinase I in Xenopus laevis.
abstract: Calcium/calmodulin (CaM) dependent protein kinase I (CaM-KI) is a member of a well-defined multi-functional CaM-K family, but its physiological and developmental functions have yet to be determined. Here, we have cloned two cDNAs encoding CaM-KI from a Xenopus laevis (X. laevis) oocyte cDNA library. One is a novel isoform of CaM-KI, named CaM-KI LiKbeta (XCaM-KI LiKbeta). The other is an alpha isoform of CaM-KI (XCaM-KIalpha), which is a highly related to previously cloned mammalian isoform. XCaM-KIalpha was constantly expressed through embryogenesis, whereas XCaM-KI LiKbeta expression dramatically increased in the neurula stage. Both XCaM-KI isoforms exhibited kinase activity in a Ca(2+)/CaM-dependent manner. Overexpression of a constitutively active mutant of CaM-KI isoforms inhibited cell cleavage in X. laevis embryos and caused a marked change of cell morphology in Hela cells. Taken together, these results suggest that CaM-KI plays a role in cell-structure regulation during early embryonic development.
authors.affiliation: Laboratory for Developmental Neurobiology, RIKEN Brain Science Institute, Saitama, Wako-shi 351-0198, Japan. saneyosi@ims.u-tokyo.ac.jp
authors.forename: Takeo
authors.initials: T
authors.lastname: Saneyoshi
authors.forename: Shoen
authors.initials: S
authors.lastname: Kume
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Mikoshiba
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D054729:Calcium-Calmodulin-Dependent Protein Kinase Type 1; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D015723:Gene Library; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D009865:Oocytes / Q000166:cytology / Q000201:enzymology; D010957:Plasmids / Q000235:genetics; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D020033:Protein Isoforms; C517596:CAMK1 protein, human; D054729:Calcium-Calmodulin-Dependent Protein Kinase Type 1; D017871:Calcium-Calmodulin-Dependent Protein Kinases