title: Steroidal compounds from in vitro regenerated shoots of Datura metel.
abstract: Regeneration of adventitious shoots from young leaves of Datura metel is described. Shoot buds developed on MS medium with 2 mg/l and elongated on hormone-free solid basal medium. The microshoots failed to produce alkaloids, but a number of steroidal compounds were detected. The C(28) sterol 3beta,24xi-dihydroxy-ergosta-5,25-dienolide, regarded as the precursor of withanolides and related steroidal lactones, was identified. The withanolide 12-deoxywithastramonolide was also detected.
authors.affiliation: Department of Botany, Calcutta University, 35 Ballygunge Circular Road, Kolkata 700019, India. bratatide@vsnl.net
authors.forename: Bratati
authors.initials: B
authors.lastname: De
mesh_terms: D001593:Benzyl Compounds / Q000494:pharmacology*; D032302:Datura / Q000737:chemistry* / Q000254:growth & development* / Q000502:physiology; D006801:Humans; D008517:Phytotherapy; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry / Q000254:growth & development / Q000502:physiology; D011687:Purines / Q000494:pharmacology*; D012038:Regeneration / Q000187:drug effects*; D013256:Steroids / Q000032:analysis*
chemical_list: D001593:Benzyl Compounds; D010937:Plant Growth Regulators; D011687:Purines; D013256:Steroids; C480551:benzylaminopurine