Anti-platelet fraction from Galega officinalis L. inhibits platelet aggregation.
A fraction from crude extract of Galega officinalis L. was purified by gel filtration on Sephadex G-25, Sepharose 4B, and ion-exchange chromatography on diethylaminoethyl (DEAE)-cellulose. The fraction with molecular weight 100-140 kDa appears to have a polysaccharide nature, including protein. The fraction inhibits platelet aggregation initiated by 25 microM adenosine 5'-diphosphate (ADP), 100 microg/ml collagen, and 0.8 U/ml thrombin with the 50% inhibiting concentration (IC(50)) being 11.2 microg/ml for ADP, and the IC(100) being 15.1 microg/ml for collagen and IC(100) 19.6 microg/ml for thrombin.
Atanasov
Atanas Todorov
AT
Department of Biophysics, Faculty of Medicine, Thracian University, Stara Zagora 6000, Bulgaria. atanastod@abv.bg
Tchorbanov
Bojidar
B
D000244:Adenosine Diphosphate; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003094:Collagen; D003636:DEAE-Cellulose; D029881:Galega / Q000737:chemistry*; D006801:Humans; D008970:Molecular Weight; D010936:Plant Extracts / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D013917:Thrombin
D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D000244:Adenosine Diphosphate; D003094:Collagen; D003636:DEAE-Cellulose; D013917:Thrombin