Structure of the main ganglioside from the brain of Xenopus laevis.
The main component of the ganglioside(1) mixture from the brain of the adult amphibian Xenopus laevis accounts for 35% of the total, as lipid bound sialic acid. This ganglioside has been purified and characterized by thin layer chromatography, partial and exhaustive enzymatic hydrolysis with sialidase, TLC-overlay procedures with anti-Gg(4)Cer and anti-Neu5Acalpha6GalNAcbeta specific monoclonal antibodies and mass spectrometry. All together the results suggest the following structure: Neu5Acalpha8Neu5Acalpha3Galbeta3(Neu5Acalpha8Neu5Acalpha6)GalNAcbeta4Galbeta4Glcbeta1Cer, or, IV(3)-alpha-Neu5Ac(2),III(6)-alpha-Neu5Ac(2)-Gg(4)Cer.
Rizzo
Angela Maria
AM
Institute of General Physiology and Biochemistry, 20134 Milan, Italy.
Berra
Bruno
B
Rossi
Federica
F
Guerra
Anna
A
Gornati
Rosalba
R
Bernardini
Giovanni
G
Taki
Takao
T
Kasama
Takeshi
T
Mauri
Laura
L
Sonnino
Sandro
S
D000818:Animals; D001923:Brain Chemistry; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000378:metabolism; D014982:Xenopus laevis
D005732:Gangliosides; D009439:Neuraminidase