title: Actinobacillus pleuropneumoniae serotype 7 siderophore receptor FhuA is not required for virulence.
abstract: A ferrichrome receptor, FhuA, was identified in Actinobacillus pleuropneumoniae serotype 7. An isogenic mutant with a deletion in the ferrichrome uptake receptor gene (fhuA) was constructed and examined in an aerosol infection model. The disease caused by the mutant was indistinguishable from disease induced by A. pleuropneumoniae serotype 7 wild-type; an isogenic mutant lacking expression of the exbB gene that is required for the uptake of transferrin-bound iron retained the ability to utilize ferrichrome, thereby indicating that an energy-coupling mechanism involved in ferrichrome transport remains to be identified.
authors.affiliation: Institut für Mikrobiologie und Tierseuchen, Tieraerztliche Hochschule Hannover, 30173 Hannover, Germany.
authors.forename: Nina
authors.initials: N
authors.lastname: Baltes
authors.forename: Walaiporn
authors.initials: W
authors.lastname: Tonpitak
authors.forename: Isabel
authors.initials: I
authors.lastname: Hennig-Pauka
authors.forename: Achim D
authors.initials: AD
authors.lastname: Gruber
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Gerlach
mesh_terms: D000189:Actinobacillus Infections / Q000382:microbiology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D000336:Aerosols; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000502:physiology*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000502:physiology*; D005291:Ferrichrome / Q000378:metabolism; D017353:Gene Deletion; D017136:Ion Transport; D007501:Iron / Q000378:metabolism; D018410:Pneumonia, Bacterial / Q000382:microbiology / Q000662:veterinary*; D011815:R Factors; D011991:Receptors, Virus / Q000235:genetics / Q000502:physiology*; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014168:Transferrin / Q000378:metabolism; D014774:Virulence / Q000235:genetics
chemical_list: D000336:Aerosols; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D018076:DNA, Complementary; D029968:Escherichia coli Proteins; C050900:FhuA protein, E coli; D011991:Receptors, Virus; D014168:Transferrin; D005291:Ferrichrome; D007501:Iron