GABAA receptor alpha 1 and beta 2 subunit null mutant mice: behavioral responses to ethanol.
Mice lacking either the alpha1 or beta 2 subunit of the GABAA receptor were tested for ethanol, saccharin, or quinine consumption, ethanol-conditioned place preference, ethanol-conditioned taste aversion, ethanol-simulated motor activity, and handling-induced seizures following chronic consumption of an ethanol liquid diet. The alpha1 null mutants showed decreased ethanol and saccharin consumption, increased aversion to ethanol, and a marked stimulation of motor activity after injection of ethanol. The beta 2 null mutants showed decreased consumption of saccharin and quinine, but not ethanol. Surprisingly, neither mutant showed marked changes in handling induced seizures before or after withdrawal of ethanol. The unique effects of deletion of these two GABAA receptor subunits on ethanol responses are discussed in terms of the distinct changes in different populations of GABAA receptors.
Blednov
Y A
YA
Waggoner Center for Alcohol and Addiction Research and Section of Neurobiology, University of Texas, Austin, TX 78712-1095, USA. yablednov@mail.utexas.edu
Walker
D
D
Alva
H
H
Creech
K
K
Findlay
G
G
Harris
R A
RA
D000428:Alcohol Drinking / Q000503:physiopathology; D000818:Animals; D001522:Behavior, Animal; D002492:Central Nervous System Depressants / Q000378:metabolism / Q000494:pharmacology*; D003214:Conditioning, Classical / Q000187:drug effects; D000431:Ethanol / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009043:Motor Activity / Q000187:drug effects*; D011963:Receptors, GABA-A / Q000235:genetics / Q000378:metabolism*
D002492:Central Nervous System Depressants; D011963:Receptors, GABA-A; D000431:Ethanol