Effect of high pressure on isomerization and degradation of lactose in alkaline media.
The combined effects of temperature (60 degrees C) and high-pressure treatments (400 MPa for 3 h) on the isomerization-degradation of lactose (10%) in basic media were studied. The formation of isomeric disaccharides (lactulose and epilactose) and galactose decreased by the application of high pressure in aqueous sodium hydroxide 4 mM (pH 10.2) and 8 mM (pH 10.6), and sodium carbonate-bicarbonate buffer (pH 10.0). In addition, no substantial color development was observed in the sodium hydroxide systems for high and atmospheric pressure, whereas the application of high pressure led to a noticeable decrease of color development in the carbonate-bicarbonate system.
Moreno
F Javier
FJ
Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
Villamiel
Mar
M
Olano
Agustín
A
D001274:Atmospheric Pressure; D002021:Buffers; D003116:Color; D004187:Disaccharides / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D007785:Lactose / Q000737:chemistry*; D007792:Lactulose / Q000737:chemistry; D011312:Pressure; D012996:Solutions; D013696:Temperature
D002021:Buffers; D004187:Disaccharides; D012996:Solutions; C047464:epilactose; D007792:Lactulose; D007785:Lactose; D005690:Galactose