Synthesis of monoglucosylated high-mannose-type dodecasaccharide, a putative ligand for molecular chaperone, calnexin, and calreticulin.
Convergent and stereoselective synthetic routes to Man9GlcNAc2 (1b), alpha-Glc1M9GlcNAc2 (2b), and its stereoisomer beta-Glc1M9GlcNAc2 (3) were established. Interaction analysis of 2b with CRT was measured by 1H NMR spectroscopy, and the first NMR-based evidence for the specific binding of CRT to 2b was obtained.
Matsuo
Ichiro
I
RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Wada
Megumi
M
Manabe
Shino
S
Yamaguchi
Yoshiki
Y
Otake
Keisuke
K
Kato
Koichi
K
Ito
Yukishige
Y
D037281:Calnexin / Q000378:metabolism*; D037282:Calreticulin / Q000378:metabolism*; D002240:Carbohydrate Sequence; D005960:Glucosides / Q000138:chemical synthesis / Q000378:metabolism; D008024:Ligands; D008358:Mannose / Q000138:chemical synthesis* / Q000378:metabolism; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000378:metabolism
D037282:Calreticulin; D005960:Glucosides; D008024:Ligands; D009844:Oligosaccharides; D037281:Calnexin; D008358:Mannose