HeLa cell adhesion on various collagen-grafted surfaces.
Cell adhesion is important to develop cell microarrays and biocompatible materials. Collagen has been reported to be able to improve cell adhesion. In this paper, two collagen coating methods (collagen grafted directly on the substrate and chitosan-modified substrate) were carried out, on which the adhesive behaviors of HeLa cells were studied. An atomic force microscope and a surface potential meter were used to characterize morphologies and electric polarization of these surfaces. It was found that surface electric polarization and the its durability and surface topography were key factors to cell adhesion. Collagen (1 mg/mL) grafted on 1% chitosan-modified surface showed the best adhesion of HeLa cell. This work might be helpful to the practical application of cell microarray chips.
Zhu
Yi
Y
National Laboratory of Molecular and Biomolecular Electronics, Southeast University, Nanjing 210096, China.
Wu
Zhonghan
Z
Tang
Zuming
Z
Lu
Zuhong
Z
D002448:Cell Adhesion / Q000502:physiology*; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D048271:Chitosan; D003094:Collagen / Q000378:metabolism*; D005898:Glass / Q000737:chemistry; D006367:HeLa Cells / Q000378:metabolism*; D006801:Humans; D018625:Microscopy, Atomic Force
D002686:Chitin; D003094:Collagen; D048271:Chitosan