title: Investigation of mitochondrial function in hereditary spastic paraparesis.
abstract: Following the association of hereditary spastic paraparesis (HSP) with mutation in the paraplegin gene (SPG7) and mitochondrial dysfunction, we wished to investigate whether mitochondrial dysfunction might be associated with other forms of HSP. Five cases of HSP caused by mutation in the spastin gene (SPG4) and nine cases with HSP with mutation in the spastin and paraplegin genes excluded (non-SPG4/SPG7), were investigated for mitochondrial dysfunction. Muscle tissue from the HSP groups and a control group was analysed histochemically and spectrophotometrically for mitochondrial dysfunction. A significant decrease in mitochondrial respiratory chain complexes I and IV was demonstrated in the non-SPG4/SPG7 group. No abnormality was detected in the SPG4 group. We therefore conclude that there is evidence for mitochondrial dysfunction in non-SPG4/SPG7 HSP. There is no evidence for mitochondrial dysfunction in the pathogenesis of spastin-related HSP.
authors.affiliation: Academic Neurology Unit, University of Sheffield, Medical School, Royal Hallamshire Hospital, UK. chrismcder@aol.com
authors.forename: C J
authors.initials: CJ
authors.lastname: McDermott
authors.forename: R W
authors.initials: RW
authors.lastname: Taylor
authors.forename: C
authors.initials: C
authors.lastname: Hayes
authors.forename: M
authors.initials: M
authors.lastname: Johnson
authors.forename: K M D
authors.initials: KM
authors.lastname: Bushby
authors.forename: D M
authors.initials: DM
authors.lastname: Turnbull
authors.forename: P J
authors.initials: PJ
authors.lastname: Shaw
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases / Q000235:genetics; D000328:Adult; D000368:Aged; D035061:Control Groups; D006651:Histocytochemistry; D006801:Humans; D008666:Metalloendopeptidases / Q000235:genetics; D008875:Middle Aged; D008931:Mitochondria, Muscle / Q000378:metabolism* / Q000502:physiology; D028361:Mitochondrial Diseases / Q000209:etiology; D018482:Muscle, Skeletal / Q000378:metabolism; D009154:Mutation; D015419:Spastic Paraplegia, Hereditary / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D000074181:Spastin; D013053:Spectrophotometry
chemical_list: D008666:Metalloendopeptidases; C112889:SPG7 protein, human; D000251:Adenosine Triphosphatases; D000074183:ATPases Associated with Diverse Cellular Activities; D000074181:Spastin; C400283:SPAST protein, human