Evaluation of hepatoprotective effect of Gentiana olivieri herbs on subacute administration and isolation of active principle.
Hepatoprotective effect of Gentiana olivieri Griseb. (Gentianaceae) flowering herbs on subacute administration were studied using in vivo models in rats. For the activity assessment on carbon tetrachloride-induced hepatic damage following biochemical parameters were evaluated; plasma and hepatic tissue malondialdehyde formation, and liver tissue glutathione level, as well as plasma transaminase enzyme levels (aspartate transferase and alanine transferase). Results of biochemical tests were also confirmed by histopathological examination. Through bioassay-guided fractionation procedures isoorientin, a known C-glycosylflavone, was isolated from the ethyl acetate fraction as the active antihepatotoxic constituent by silica gel column chromatography. Isoorientin exhibited significant hepatoprotective effect at 15 mg/kg b.w. dose.
Orhan
Didem Deliorman
DD
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler 06330, Ankara, Turkey
Aslan
Mustafa
M
Aktay
Göknur
G
Ergun
Ender
E
Yesilada
Erdem
E
Ergun
Fatma
F
D000284:Administration, Oral; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D005591:Chemical Fractionation; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000473:pathology / Q000517:prevention & control*; D018890:Chemoprevention; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D030012:Gentiana / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D047311:Luteolin; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D020011:Protective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley
D005419:Flavonoids; D010936:Plant Extracts; D020011:Protective Agents; D008315:Malondialdehyde; C057912:homoorientin; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; D047311:Luteolin