Cationic electrophoresis and electrotransfer of membrane glycoproteins.
Sodium dodecyl sulfate electrophoresis of glycoproteins results in broad, fuzzy bands. The use of the positively charged detergent cetyltrimethylammonium bromide (CTAB) avoids this problem. Protocols for CTAB electrophoresis, electroblotting, and protein detection are presented. These methods were found useful in studies on the multiple drug resistance ATPase Mdr1.
Buxbaum
Engelbert
E
Department of Biochemistry, Faculty of Medicine, University of Kuwait, Safat, 13110, Kuwait. engelbert_buxbaum@web.de
D000818:Animals; D015153:Blotting, Western; D016466:CHO Cells; D002412:Cations / Q000737:chemistry*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008562:Membrane Glycoproteins / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling / Q000379:methods
D002412:Cations; D002593:Cetrimonium Compounds; D008562:Membrane Glycoproteins; D012967:Sodium Dodecyl Sulfate; D000077286:Cetrimonium