title: Osmotic stress increases cullin-5 (cul-5) mRNA in the rat cerebral cortex, hypothalamus and kidney.
abstract: Cullin-5 (cul-5), a member of the cullin gene family, may have a role in proteolysis and cell cycle regulation. Our recent study demonstrated that cul-5 mRNA is ubiquitously expressed in the central nervous system and many peripheral organs. The present study used quantitative realtime polymerase chain reaction to measure changes in cul-5 mRNA expression as a consequence of osmotic stress in vivo. Cul-5 mRNA levels were significantly increased in the rat cerebral cortex, hypothalamus and kidney following 48 h of water deprivation. Water deprivation for a shorter time period (24 h) or rehydration (24 h access to water following 48 h of water deprivation) also elevated kidney cul-5 mRNA levels. Water deprivation did not significantly alter cul-5 mRNA levels in the brainstem, cerebellum, hippocampus, lung or liver. Since cul-5 appears to be linked to proteosome-mediated protein degradation, it may have a role in protein regulation under conditions of osmotic stress.
authors.affiliation: Graduate Program in Neuroscience, F. Edmund Hébert School of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, 20814, Bethesda, MD, USA. tceremuga@usuhs.mil
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Ceremuga
authors.forename: Xiang Lan
authors.initials: XL
authors.lastname: Yao
authors.forename: Yang
authors.initials: Y
authors.lastname: Xia
authors.forename: Dhritiman
authors.initials: D
authors.lastname: Mukherjee
authors.forename: Joseph T
authors.initials: JT
authors.lastname: McCabe
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics; D002540:Cerebral Cortex / Q000378:metabolism*; D044842:Cullin Proteins; D007031:Hypothalamus / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D009997:Osmotic Pressure; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D020318:Rats, Long-Evans; D017483:Receptors, Vasopressin / Q000096:biosynthesis* / Q000235:genetics; D014868:Water Deprivation / Q000502:physiology; D014882:Water-Electrolyte Balance / Q000235:genetics / Q000502:physiology*
chemical_list: D018797:Cell Cycle Proteins; C094547:Cul5 protein, rat; D044842:Cullin Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D017483:Receptors, Vasopressin