Towards a unified system for detecting waterborne pathogens.
Currently, there is no single method to collect, process, and analyze a water sample for all pathogenic microorganisms of interest. Some of the difficulties in developing a universal method include the physical differences between the major pathogen groups (viruses, bacteria, protozoa), efficiently concentrating large volume water samples to detect low target concentrations of certain pathogen groups, removing co-concentrated inhibitors from the sample, and standardizing a culture-independent endpoint detection method. Integrating the disparate technologies into a single, universal, simple method and detection system would represent a significant advance in public health and microbiological water quality analysis. Recent advances in sample collection, on-line sample processing and purification, and DNA microarray technologies may form the basis of a universal method to detect known and emerging waterborne pathogens. This review discusses some of the challenges in developing a universal pathogen detection method, current technology that may be employed to overcome these challenges, and the remaining needs for developing an integrated pathogen detection and monitoring system for source or finished water.
Straub
Timothy M
TM
Analytical Microbiology Group, Pacific Northwest National Laboratory, 902 Battelle Boulevard, P.O. Box 999 MSIN P7-50, Richland, WA 99352, USA. Timothy.Straub@pnl.gov
Chandler
Darrell P
DP
D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D003140:Communicable Disease Control; D004784:Environmental Monitoring / Q000379:methods*; D056890:Eukaryota / Q000235:genetics / Q000302:isolation & purification; D006376:Helminths / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007239:Infections / Q000209:etiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification; D014867:Water / Q000469:parasitology*; D014871:Water Microbiology; D014881:Water Supply / Q000592:standards
D014867:Water