B-cell commitment: deciding on the players.
Considerable progress has been made over the past few years in determining the key molecular players that regulate the commitment and development of early B-lineage cells in mouse bone marrow (transcription factors, cytokines and their receptors). However, confusion remains as to the precise stages, surface phenotypes and lineage restrictions that are observed early in B-cell development. Increasingly powerful flow cytometry analysis of genetically altered animals (knockouts and transgenics) combined with sensitive functional assays may provide an answer, a "scorecard" for the cell stages.
Hardy
Richard R
RR
Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA. rr_hardy@fccc.edu
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology*; D008565:Membrane Proteins / Q000502:physiology; D051379:Mice; D008954:Models, Biological; D011518:Proto-Oncogene Proteins / Q000502:physiology; D051880:Receptors, Notch; D014157:Transcription Factors / Q000502:physiology; D051153:Wnt Proteins; D029961:Zebrafish Proteins
D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; D051880:Receptors, Notch; D014157:Transcription Factors; D051153:Wnt Proteins; D029961:Zebrafish Proteins