CesD2 of enteropathogenic Escherichia coli is a second chaperone for the type III secretion translocator protein EspD.
Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli are extracellular pathogens that employ a type III secretion system to export translocator and effector proteins, proteins which facilitates colonization of the mucosal surface of the intestine via formation of attaching and effacing (A/E) lesions. The genes encoding the proteins for A/E lesion formation are located on a pathogenicity island, termed the locus of enterocyte effacement (LEE), which contains eae encoding intimin as well as the type III secretion system and effector genes. Many type III secreted proteins are stabilized and maintained in a secretion-competent conformation in the bacterial cytosol by specific chaperone proteins. Three type III chaperones have been described thus far within the EPEC LEE region: CesD, for the translocator proteins EspB and EspD; CesT, for the effector proteins Tir and Map; and CesF, for EspF. In this study we report the characterization of CesD2 (previously Orf27), a second LEE-encoded chaperone for EspD. We show specific CesD2-EspD protein interaction which appears to be necessary for proper EspD secretion in vitro and pathogenesis in vivo as demonstrated in the A/E-lesion-forming mouse pathogen Citrobacter rodentium.
Neves
Bianca C
BC
Centre for Molecular Microbiology and Infection, Flowers Building, Department of Biological Sciences, Imperial College London, London SW7 2AZ, UK.
Mundy
Rosanna
R
Petrovska
Liljana
L
Dougan
Gordon
G
Knutton
Stuart
S
Frankel
Gad
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016971:Citrobacter freundii / Q000472:pathogenicity; D003106:Colon / Q000473:pathology; D004756:Enterobacteriaceae Infections / Q000473:pathology; D004926:Escherichia coli / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010750:Phosphoproteins; D017422:Sequence Analysis, DNA; D014774:Virulence
C473781:CesD2 protein, E coli; D029968:Escherichia coli Proteins; C474481:EspD protein, E coli; C457340:LEE protein, E coli; D008565:Membrane Proteins; D018832:Molecular Chaperones; D010750:Phosphoproteins