Ganglioside binding pattern of CD33-related siglecs.
Our study deals with the interaction of CD33 related-siglecs-5,-7,-8,-9,-10 with gangliosides GT1b, GQ1b, GD3, GM2, GM3 and GD1a. Siglec-5 bound preferentially to GQ1b, but weakly to GT1b, whereas siglec-10 interacted only with GT1b ganglioside. Siglec-7 and siglec-9 displayed binding to gangliosides GD3, GQ1b and GT1b bearing a disialoside motif, though siglec-7 was more potent; besides, siglec-9 interacted also with GM3. Siglec-8 demonstrated low affinity to the gangliosides tested compared with other siglecs. Despite high structural similarity of CD33 related siglecs, they demonstrated different ganglioside selectivity, in particular to the Neu5Acalpha2-8Neu5Ac motif.
Rapoport
Eugenia
E
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia.
Mikhalyov
Ilya
I
Zhang
Jiquan
J
Crocker
Paul
P
Bovin
Nicolai
N
D015703:Antigens, CD / Q000737:chemistry / Q000378:metabolism*; D000944:Antigens, Differentiation, B-Lymphocyte; D015214:Antigens, Differentiation, Myelomonocytic / Q000737:chemistry / Q000378:metabolism*; D002240:Carbohydrate Sequence; D005678:G(M2) Ganglioside; D005679:G(M3) Ganglioside; D005732:Gangliosides / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D037102:Lectins; D011485:Protein Binding; D011956:Receptors, Cell Surface; D063268:Sialic Acid Binding Ig-like Lectin 3; D063265:Sialic Acid Binding Immunoglobulin-like Lectins
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D015214:Antigens, Differentiation, Myelomonocytic; C571163:CD33 protein, human; D005679:G(M3) Ganglioside; D005732:Gangliosides; D037102:Lectins; D011956:Receptors, Cell Surface; C426228:SIGLEC10 protein, human; C114531:SIGLEC5 protein, human; C402206:SIGLEC7 protein, human; C403967:SIGLEC8 protein, human; C412227:SIGLEC9 protein, human; D063268:Sialic Acid Binding Ig-like Lectin 3; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; C021298:ganglioside, GD1a; D005678:G(M2) Ganglioside; C016481:trisialoganglioside GT1; C026226:ganglioside, GD3; C039129:GQ1b ganglioside