title: Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases.
abstract: A significant number of chimeric 16S rDNA sequences of diverse origin were identified in the public databases by partial treeing analysis. This suggests that chimeric sequences, representing phylogenetically novel non-existent organisms, are routinely being overlooked in molecular phylogenetic surveys despite a general awareness of PCR-generated artefacts amongst researchers.
authors.affiliation: ComBinE group, Advanced Computational Modelling Centre, The University of Queensland, Brisbane 4072, Australia.
authors.forename: Philip
authors.initials: P
authors.lastname: Hugenholtz
authors.affiliation: ComBinE group, Advanced Computational Modelling Centre, The University of Queensland, Brisbane 4072, Australia.
authors.forename: Thomas
authors.initials: T
authors.lastname: Huber
mesh_terms: D002678:Chimera / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D030561:Databases, Nucleic Acid; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S