Delayed reoxygenation after maximal isometric handgrip exercise in high oxidative capacity muscle.
We hypothesized that after maximal short-term isometric exercise, when O(2) demand is still high and O(2) supply is not fully activated, higher oxidative capacity muscle may exhibit slower muscle reoxygenation after the exercise than low oxidative capacity muscle. Seven healthy male subjects performed a maximal voluntary isometric handgrip exercise for 10 s. The reoxygenation rate after the exercise (Reoxy-rate) in the finger flexor muscle was determined by near infrared continuous wave spectroscopy (NIRcws) while phosphocreatine (PCr) was measured simultaneously by (31)P magnetic resonance spectroscopy. Muscle oxygen consumption (muscle VO(2)) and muscle oxidative capacity were evaluated using the rate of PCr resynthesis post-exercise. The forearm blood flow (FBF) index at the end of exercise was measured using NIRcws. There was a significant positive correlation between the Reoxy-rate, which ranged between 0.53% s(-1) and 12.47% s(-1), and the time constant for PCr resynthesis, which ranged between 17.8 s and 38.3 s (r(2)=0.939, P<0.001). At the end of the exercise, muscle VO(2) exceeded the resting level by approximately 25-fold, while the FBF index exceeded the resting level by only 3-fold on average. The Reoxy-rate closely correlated with muscle VO(2) (r(2)=0.727, P<0.05), but not with the FBF index. Also, the estimated O(2) balance (muscle VO(2) index/FBF index) was negatively correlated with the Reoxy-rate (r(2)=0.820, P<0.001). These results support our hypothesis that higher oxidative capacity muscle shows slower muscle reoxygenation after maximal short-term isometric exercise because the Reoxy-rate after this type of exercise may be influenced more by muscle VO(2) than by O(2) supply.
Kime
Ryotaro
R
Department of Biochemistry and Biophysics, 250 Anatomy-Chemistry Building, University of Pennsylvania, 37th and Hamilton Walk, Philadelphia, PA 19104-6059, USA. ZXC06104@nifty.com
Hamaoka
Takafumi
T
Sako
Takayuki
T
Murakami
Motohide
M
Homma
Toshiyuki
T
Katsumura
Toshihito
T
Chance
Britton
B
D000222:Adaptation, Physiological / Q000502:physiology*; D000328:Adult; D015444:Exercise / Q000502:physiology*; D005385:Fingers / Q000502:physiology; D018737:Hand Strength; D006706:Homeostasis / Q000502:physiology; D006801:Humans; D007537:Isometric Contraction / Q000502:physiology*; D008279:Magnetic Resonance Imaging; D008297:Male; D018482:Muscle, Skeletal / Q000502:physiology*; D009211:Myoglobin / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D010108:Oxyhemoglobins / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism*; D005082:Physical Exertion / Q000502:physiology; D019265:Spectroscopy, Near-Infrared
D009211:Myoglobin; D010108:Oxyhemoglobins; C017595:oxymyoglobin; D010725:Phosphocreatine; D010100:Oxygen