A temperature-dependent interaction of neutral red with calf thymus DNA.
Neutral red (NR) is used as a probe to study the temperature and concentration dependent interaction of a cationic dye with nucleic acid. A temperature-dependent interaction of NR with calf thymus DNA (CT DNA) has been studied by differential pulse voltammetry (DPV), UV-Visible absorption, circular dichroism (CD) and fluorescence spectroscopy. The experimental results of increasing peak current, changes in the UV-Visible absorption and fluorescence spectra of NR and decreasing the induced circular dichroism (ICD) intensity show that (i) the binding mode of NR molecules is changed from intercalating into DNA base pairs to aggregating along the DNA double helix and (ii) the orientation of NR chromophore in DNA double helix is also changed with the temperature.
Wang
Zhenxin
Z
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 30022 Jilin, China.
Zhang
Zheling
Z
Liu
Dianjun
D
Dong
Shaojun
S
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D009499:Neutral Red / Q000737:chemistry / Q000378:metabolism*; D013053:Spectrophotometry; D013696:Temperature; D013950:Thymus Gland / Q000378:metabolism
D009499:Neutral Red; D004247:DNA