title: Characterization of complementary deoxyribonucleic acids encoding preprogalanin and its alternative splice variants in the goldfish.
abstract: This paper reports the identification of five preprogalanin complementary deoxyribonucleic acids encoding preprogalanin peptides in the goldfish. Preprogalanin 1A, 1B and 1C are encoded in galanin gene 1 and 2A and 2B are encoded in gene 2. Preprogalanin 1B and 2B have a 24 amino acids insert in the mature peptide region and form 1C has a deletion of 23 amino acids in the middle of the galanin message associated peptide region. The mature peptides from the preprogalanin 1A and 1C are 29 amino acids. However, the mature peptide is 31 amino acids from preprogalanin 2A, 53 amino acids from 1B and 55 amino acids from 2B. The physiological significance of multiple forms of galanin peptide is unknown. Organization of galanin gene 1, which is similar to the mammalian galanin gene has been identified. Expression of preprogalanin messenger ribonucleic acids was widely detected in goldfish brain and several peripheral tissues.
authors.affiliation: Department of Biological Sciences, University of Alberta, Edmonton, Alta., Canada T6G 2E9.
authors.forename: Surajlal
authors.initials: S
authors.lastname: Unniappan
authors.forename: Xinwei
authors.initials: X
authors.lastname: Lin
authors.forename: Richard E
authors.initials: RE
authors.lastname: Peter
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000166:cytology / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D019004:Galanin / Q000235:genetics* / Q000378:metabolism; D006054:Goldfish / Q000235:genetics*; D008297:Male; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D021382:Protein Sorting Signals / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D011498:Protein Precursors; D021382:Protein Sorting Signals; D019004:Galanin