title: Changes in blood lipid peroxidation markers and antioxidants after a single sprint anaerobic exercise.
abstract: It has been well demonstrated that the principal factor responsible for oxidative damage during exercise is the increase in oxygen consumption. However, other theoretical factors (acidosis, catecholamine autoxidation, ischemia-reperfusion syndrome, etc.) that are known to induce, in vitro, oxidative damage may also be operative during short-term supramaximal anaerobic exercise. Therefore, we hypothesized that short-term supramaximal anaerobic exercise (30-s Wingate test) could induce an oxidative stress. Lipid peroxidation markers [serum lipid radical production detected by electron spin resonance (ESR) spectroscopy and plasma malondialdehyde (MDA) levels detected by the thiobarbituric acid reactive substances (TBARS) method], as well as erythrocyte antioxidant enzyme activities [glutathione peroxidase (GPx), superoxide dismutase (SOD)] and erythrocyte glutathione (GSH) levels, were measured at rest, after the Wingate test and during the 40 min of recovery. The recovery of exercise was associated with a significant increase (x2.7) in lipid radical production detected by ESR spectroscopy, as well as with changes in the erythrocyte GSH level (-13.6%) and SOD activity (-11.7%). The paradoxical decrease in plasma TBARS (-23.7%) which was correlated with the peak power developed during the Wingate test ( r=-0.7), strongly suggests that such exercise stimulates the elimination of MDA. In conclusion, this study demonstrates that short-term supramaximal anaerobic exercise induces an oxidative stress and that the plasma TBARS level is not a suitable marker during this type of exercise.
authors.affiliation: Laboratoire de Physiologie et de Biomécanique de l'Exercice Musculaire, Campus la Harpe, UFRAPS Rennes 2, EA 1274, Avenue Charles Tillon, CS 24414, 35044 Rennes Cedex, France. carole.groussard@uhb.fr
authors.forename: C
authors.initials: C
authors.lastname: Groussard
authors.forename: F
authors.initials: F
authors.lastname: Rannou-Bekono
authors.forename: G
authors.initials: G
authors.lastname: Machefer
authors.forename: M
authors.initials: M
authors.lastname: Chevanne
authors.forename: S
authors.initials: S
authors.lastname: Vincent
authors.forename: O
authors.initials: O
authors.lastname: Sergent
authors.forename: J
authors.initials: J
authors.lastname: Cillard
authors.forename: A
authors.initials: A
authors.lastname: Gratas-Delamarche
mesh_terms: D000328:Adult; D000693:Anaerobiosis / Q000502:physiology; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D004789:Enzyme Activation; D004912:Erythrocytes / Q000378:metabolism*; D005080:Exercise Test; D017079:Exercise Tolerance / Q000502:physiology*; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008055:Lipids / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000097:blood* / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D008055:Lipids; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione