title: Relationships between plasma concentrations of leptin and other metabolic hormones in GH-transgenic sheep infused with glucose.
abstract: To study the regulation of leptin secretion in sheep, we infused glucose (0.32 g/h/kg for 12 h) into GH-transgenic animals (n = 8) that have chronically high plasma concentrations of ovine GH and insulin, but low body condition and low plasma leptin concentrations, and compared the responses with those in controls (n = 8). In both groups, the infusion increased plasma concentrations of glucose and insulin within 1 h and maintained high levels throughout the infusion period (P < 0.0001). Compared with controls, GH-transgenics had higher concentrations of insulin, IGF-1, GH (all P < 0.0001) and cortisol (P < 0.05), but lower GH pulse frequency (P < 0.0001). Overall, leptin concentrations were lower in GH-transgenics than in controls (P < 0.01). A postprandial increase in leptin concentrations was observed in both groups, independently of glucose treatment, after which the values remained elevated in animals infused with glucose, but returned to basal levels in those infused with saline, independently of transgene status. In both GH-transgenics and controls, glucose infusion did not affect the concentrations of GH, IGF-1, or cortisol. In conclusion, GH-transgenic and control sheep show similar responses to glucose infusion for leptin and other metabolic hormones, despite differences between them in body condition and basal levels of these hormones. Glucose, insulin, GH, IGF-1 and cortisol are probably not major factors in the acute control of leptin secretion in sheep, although sustained high concentrations of GH and IGF-1 might reduce adipose tissue mass or inhibit leptin gene expression.
authors.affiliation: CSIRO Division of Livestock Industries, Private Bag #5, PO Wembley, WA 6913, Australia. hiroya@affrc.go.jp
authors.forename: H
authors.initials: H
authors.lastname: Kadokawa
authors.forename: J R
authors.initials: JR
authors.lastname: Briegel
authors.forename: M A
authors.initials: MA
authors.lastname: Blackberry
authors.forename: D
authors.initials: D
authors.lastname: Blache
authors.forename: G B
authors.initials: GB
authors.lastname: Martin
authors.forename: N R
authors.initials: NR
authors.lastname: Adams
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001786:Blood Glucose / Q000032:analysis; D005260:Female; D005947:Glucose / Q000008:administration & dosage*; D013006:Growth Hormone / Q000097:blood / Q000235:genetics*; D006854:Hydrocortisone / Q000097:blood; D007328:Insulin / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D020738:Leptin / Q000097:blood*; D008297:Male; D012756:Sheep / Q000097:blood* / Q000235:genetics
chemical_list: D001786:Blood Glucose; D007328:Insulin; D020738:Leptin; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D005947:Glucose; D006854:Hydrocortisone