title: The rapid detection of low molecular mass proteins differentially expressed under biological stress for four Helicobacter spp. using ProteinChip technology.
abstract: Helicobacter pylori is one of the most prevalent human pathogens in the world and is the aetiological agent of gastritis, peptic ulcer disease and gastric malignancies. In addition H. pylori and other novel members of the genus are capable of successfully colonising the bile-rich niche of the upper intestine and are associated with a diverse range of intestinal pathologies. Surface-enhanced laser desorption/ionisation-time of flight mass spectrometry was used to analyse surface extracts from H. pylori, Helicobacter bilis, Helicobacter pullorum and "Helicobacter sp. flexispira" to characterise cell surface changes following bile stress. The system detected two distinct response patterns to bile stress on the cell surface of Helicobacter spp. in vitro. The first involved the increase under bile stress of peaks at 7.6 and 7.9 kDa for H. billis and H. pullorum, respectively. In contrast both "Helicobacter sp. flexispira" and a clinical isolate of H. pylori had similar response profiles to bile stress. Both strains had at least three low mass peaks decreased under bile stress and a single peak induced by bile stress. The present study has established the use of ProteinChip(R) technology to analyse helicobacter-related proteomics. Specifically this study has established that different patterns are generated in response to bile stress among various pathogenic Helicobacter spp. which may give insights into the ability of these strains to colonise different niches.
authors.affiliation: Department of Medical Microbiology, Dermatology and Infection, Lund University, Sweden.
authors.forename: Sean O
authors.initials: SO
authors.lastname: Hynes
authors.forename: Jim
authors.initials: J
authors.lastname: McGuire
authors.forename: Tobias
authors.initials: T
authors.lastname: Falt
authors.forename: Torkel
authors.initials: T
authors.lastname: Wadström
mesh_terms: D001646:Bile / Q000737:chemistry*; D005756:Gastritis / Q000382:microbiology; D016998:Helicobacter / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics; D013045:Species Specificity
chemical_list: D011506:Proteins