Imaging TOF-SIMS analysis of oligonucleotide microarrays.
Single strand thiolated oligonucleotide (25-mer) was printed onto chemically modified glass and silicon surfaces. Confirmation of the level of attachment attained in each case was effected through detection by conventional confocal fluorescence microscopy. Both positive-ion and negative ion imaging time-of-flight mass spectra were recorded for the visualization of micro-patterned oligonucleotide arrays. This represents the first report of such detection by this form of mass spectrometry on glass. Ultimately, we are interested in the possibility that imaging time-of-flight secondary ion mass spectrometry can discern the orientation and conformation of DNA strands present on the surface of a substrate.
Cheran
Larisa-Emilia
LE
Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6.
Vukovich
Dajana
D
Thompson
Michael
M
D006801:Humans; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods