Synthesis and characterization of covalently linked single-stranded DNA oligonucleotide-dendron conjugates.
A solution-phase synthesis and characterization of covalent DNA-dendron conjugates is presented. Thiol-terminated 12-base oligonucleotides were added to second- and third-generation triazine-based dendrons via thiol/disulfide exchange chemistry. Single-stranded DNA oligonucleotides were successfully attached to dendrons at the core, the periphery, and both. Proof of structure for these architectures is derived primarily from mass spectrometry and polyacrylamide gel electrophoresis and complemented by labeling analysis using Ellman's reagent and degradation analysis using a reducing agent.
Bell
Stephen A
SA
Department of Chemistry, Texas A&M University, P.O. Box 30012, College Station 77842-3012, USA.
McLean
Megan E
ME
Oh
Sang-Keun
SK
Tichy
Shane E
SE
Zhang
Wen
W
Corn
Robert M
RM
Crooks
Richard M
RM
Simanek
Eric E
EE
D004396:Coloring Agents; D004277:DNA, Single-Stranded / Q000138:chemical synthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009841:Oligonucleotides / Q000138:chemical synthesis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004396:Coloring Agents; D004277:DNA, Single-Stranded; D007202:Indicators and Reagents; D009841:Oligonucleotides