Vasopressin-SV40 T antigen expression in transgenic mice induces brain tumor and lymphoma.
In order to understand the importance of various cis-acting elements in regulating VP gene expression, transgenic mice regulated by VP constructs were produced containing 3.8 kb of the 5' flanking region and all the exons and introns in the mouse VP gene, which was fused at the end of exon 3 to an SV40 T antigen (Tag). In the transgenic mice by the pVPSV.IGR3.6 construct, all the six transgenic mice died at the age of 2-6 weeks. In the transgenic mice by pVPSV.IGR2.1, 21% of them had brain tumors at 5 weeks and 100% of the mice had brain tumors after 24 weeks. Histological analysis of the transgenic mice revealed primitive neuroectodermal tumors (PNET) in the brain and lymphoma in the spleen and lymph nodes. The phenotype differences between the two transgenic mice suggest that tissue-specific expression might be regulated by cis-acting elements in the 1.5-kb of the 3(') flanking region, which are not contained in pVPSV.IGR2.1. In conclusion, pVPSV.IGR2.1 mice will be a valuable mouse model system for investigating PNET tumorigenesis in the brain and lymphoma in the lymph nodes and spleen.
Lee
Jeong Woong
JW
Laboratory Animal Center, Catholic Research Institutes of Medical Science, Catholic Medical College, 505 Banpo-Dong, Seocho-Ku, Seoul 137-701, Republic of Korea.
Park
Jun Hong
JH
Kim
Kil Soo
KS
Lee
Eun Ju
EJ
Kim
Myoung Ok
MO
Kim
Sung Hyun
SH
Jeong
Seong Whan
SW
Kim
Chul Woo
CW
Lee
Ho Joon
HJ
Kang
Kyung Sun
KS
Chang
Kyu Tae
KT
Hyun
Byung Hwa
BH
Ryoo
Zae Young
ZY
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000378:metabolism*; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008223:Lymphoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism / Q000473:pathology; D014018:Tissue Distribution; D019076:Transgenes; D014667:Vasopressins / Q000235:genetics / Q000378:metabolism*
D000952:Antigens, Polyomavirus Transforming; D011993:Recombinant Fusion Proteins; D014667:Vasopressins