Anti-inflammatory effect of Lonicera japonica in proteinase-activated receptor 2-mediated paw edema.
BACKGROUND
Lonicera japonica (Caprifoliaceae) has long been used for treatment of infectious diseases. In the present study, the anti-inflammatory effects of L. japonica water extract (AELJ) were investigated in proteinase-activated receptor 2 (PAR2)-mediated mouse paw edema.


METHODS
Paw edema was induced by injection of trypsin or trans-cinnamoyl-LIGRLO-NH(2) (tc-NH(2)) into hindpaw of mice. AELJ (10, 50, 100, and 200 mg/kg) was orally administered 1 h before induction of inflammation.


RESULTS
At doses of 50, 100 and 200 mg/kg, the AELJ showed significant inhibition of both change in paw thickness and vascular permeability. The AELJ (100 mg/kg) also significantly inhibited PAR2 agonists-induced myeloperoxidase (MPO) activity and tumor necrosis factor (TNF)-alpha expression in paw tissue.


CONCLUSIONS
The present study demonstrated that AELJ has an anti-inflammatory action for PAR2-mediated paw edema.
Tae
Jin
J
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 570-749, South Korea.
Han
Seung-Woo
SW
Yoo
Jae-Young
JY
Kim
Jin-A
JA
Kang
Ok-Hwa
OH
Baek
Ok-Seon
OS
Lim
Jong-Pil
JP
Kim
Dae-Ki
DK
Kim
Young-Ho
YH
Bae
Ki-Hwan
KH
Lee
Young-Mi
YM
D000284:Administration, Oral; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002934:Cinnamates; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006614:Hindlimb / Q000187:drug effects; D031239:Lonicera / Q000737:chemistry*; D051379:Mice; D009195:Peroxidase / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D044464:Receptor, PAR-2 / Q000819:agonists / Q000378:metabolism*; D014357:Trypsin; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000187:drug effects
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002934:Cinnamates; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D044464:Receptor, PAR-2; D014409:Tumor Necrosis Factor-alpha; D009195:Peroxidase; D014357:Trypsin