Reduction of aflatoxins by Korean soybean paste and its effect on cytotoxicity and reproductive toxicity--part 2. Antigenotoxic effects of the methanol extract of Korean soybean paste (doen-jang) on aflatoxin B1-induced bacterial reverse mutation and chromosome aberration.
A bacterial reverse-mutation assay with Salmonella Typhimurium TA1535, TA1537, TA98, TA100, and TA102 and an in vitro chromosome aberration assay with Chinese hamster lung (CHL) cells were used to investigate the genotoxicity of the methanol extract of Korean soybean paste (doen-jang [dwen-jahng]) and its antigenotoxic activity against aflatoxin B1. The methanol extract revealed nonmutagenic potential for all of the bacterial strains tested. The extract significantly reduced the numbers of revertants per plate when it was added to the assay system with Salmonella Typhimurium TA100 (P < 0.05). The extract also exhibited significant inhibitory effects on chromosome aberration in CHL cells (P < 0.05). The findings of this work indicate that the methanol extract of Korean soybean paste could have strong potential as an antigenotoxic material.
Kim
Jong-Gyu
JG
Department of Public Health, Keimyung University, 1000 Shindang-Dong, Dalseo-Gu, Daegu 704-701, Korea. jgkim@kmu.ac.kr
Lee
Yong-Wook
YW
Shintani
Hideharu
H
D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001230:Aspergillus / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations; D006224:Cricetinae; D007723:Korea; D000432:Methanol / Q000737:chemistry; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*; D013025:Glycine max / Q000737:chemistry*
D016587:Antimutagenic Agents; D009153:Mutagens; D010936:Plant Extracts; D016604:Aflatoxin B1; D000432:Methanol