title: Regulation of the human Fc epsilon RI alpha-chain distal promoter.
abstract: The alpha-chain of the high affinity receptor for IgE (Fc epsilon RI) is essential for cell surface expression of Fc epsilon RI and binding of the IgE Ab. The human alpha-chain gene possesses two promoters: the proximal promoter, which is highly conserved with that of rodent; and the distal promoter, the structure and role of which are largely unknown. Transcriptional regulation of the alpha-chain distal promoter was investigated in this study. Transient reporter assay revealed critical region for transcription activity located within -27/-17. EMSA identified Elf-1, YY1, and PU.1 as transcription factors binding to this region. In contrast to the proximal promoter, which was trans-activated by YY1 and PU.1, these transcription factors exhibited repressive function on this promoter. Addition of IL-4 caused a marked increase in transcription from the distal promoter and subsequently increased the intracellular production of the alpha-chain. These results indicate that IL-4-dependent up-regulation of the human alpha-chain was due to enhancement of distal promoter activity and suggests that the two promoters have different regulatory mechanisms for alpha-chain expression.
authors.affiliation: Atopy (Allergy) Research Center, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Masanari
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Chiharu
authors.initials: C
authors.lastname: Nishiyama
authors.forename: Makoto
authors.initials: M
authors.lastname: Nishiyama
authors.forename: Yushiro
authors.initials: Y
authors.lastname: Akizawa
authors.forename: Kyoko
authors.initials: K
authors.lastname: Takahashi
authors.forename: Tomonobu
authors.initials: T
authors.lastname: Ito
authors.forename: Susumu
authors.initials: S
authors.lastname: Furukawa
authors.forename: Chisei
authors.initials: C
authors.lastname: Ra
authors.forename: Ko
authors.initials: K
authors.lastname: Okumura
authors.forename: Hideoki
authors.initials: H
authors.lastname: Ogawa
mesh_terms: D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology; D050981:Erythroid-Specific DNA-Binding Factors; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology*; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000276:immunology*; D011518:Proto-Oncogene Proteins / Q000302:isolation & purification / Q000378:metabolism; D017455:Receptors, IgE / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid / Q000276:immunology; D012097:Repressor Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology; D015534:Trans-Activators / Q000302:isolation & purification / Q000378:metabolism; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism; D024363:Transcription Initiation Site; D014407:Tumor Cells, Cultured; D050986:YY1 Transcription Factor; D000099232:Proto-Oncogene Protein Spi-1
chemical_list: D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D015847:Interleukin-4; D011518:Proto-Oncogene Proteins; D017455:Receptors, IgE; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D050986:YY1 Transcription Factor; D000099232:Proto-Oncogene Protein Spi-1; C494698:YY1 protein, human