title: A comparison of the in vitro cytotoxicity of two root canal sealers.
abstract: The purpose of this study was to compare the cytotoxicity of an epoxy resin-based sealer (AH Plus) and a silicone-based sealer (Roeko Seal Automix, RSA). Cytotoxicity was assessed using the MTT assay for mitochondrial enzyme activity and haemocytometer viable cell counting after 24, 48 and 72-h exposure to L929 cells. AH Plus and RSA showed no statistically significant difference in the degree of toxicity. Both sealers had a low toxic influence on the cells during the experimental period. This study indicates that epoxy resin-based sealer AH Plus and the silicone-based sealer RSA have similar levels of cytotoxicity to mouse fibroblasts.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Ankara University, Ankara, Turkey. mdartar@hotmail.com
authors.forename: M Dartar
authors.initials: MD
authors.lastname: Oztan
authors.forename: S
authors.initials: S
authors.lastname: Yilmaz
authors.forename: A
authors.initials: A
authors.lastname: Kalayci
authors.forename: L
authors.initials: L
authors.lastname: Zaimoğlu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials; D002478:Cells, Cultured / Q000187:drug effects*; D004853:Epoxy Resins / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects*; D051379:Mice; D009152:Mutagenicity Tests; D012387:Root Canal Filling Materials / Q000633:toxicity*; D012828:Silicones / Q000633:toxicity*
chemical_list: D001672:Biocompatible Materials; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; D012828:Silicones; C534916:epoxy resin-based root canal sealer