[An organotypic culture of the newt retina together with other tissues of the posterior eye segment as a model for studying the effects of the cell adhesion glycoproteins].
The adult newt retina explanted together with the posterior eye wall and cultivated for a short time in a serum-free medium was tested as an experimental model by several criteria, including the expression of protein markers of the main retinal cell types. Some differences in the expression of specific photoreceptor, interneuron, and glial cell proteins and the localization of acetylcholinesterase activity were found to appear during in vitro cultivation. Using this model, preliminary tests of new cell adhesion glycoproteins from the bovine retina and pigment epithelium were conducted, and the role of pigment epithelial cell proteins in improving cell viability in the cultivated newt retina was revealed. Moreover, the fraction of basic adhesion proteins from the bovine pigment epithelium improved the survival potential of the macroglial (Muller) cell population, compared to that in the control.
Krasnov
M S
MS
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia.
Grigorian
E N
EN
Iamskova
V P
VP
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D016895:Culture Media, Serum-Free; D005123:Eye / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D005136:Eye Proteins; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006023:Glycoproteins / Q000494:pharmacology*; D051597:Hippocalcin; D008074:Lipoproteins; D008297:Male; D008954:Models, Biological; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D009924:Organ Culture Techniques / Q000379:methods*; D010857:Pigment Epithelium of Eye / Q000737:chemistry / Q000378:metabolism; D010999:Pleurodeles; D019736:Prostheses and Implants; D051603:Recoverin; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D015415:Biomarkers; D002135:Calcium-Binding Proteins; D016895:Culture Media, Serum-Free; D005136:Eye Proteins; D005904:Glial Fibrillary Acidic Protein; D006023:Glycoproteins; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; C051774:neurofilament protein H; D051603:Recoverin; D051597:Hippocalcin; D000110:Acetylcholinesterase