In vitro effect of light-cure dental adhesive on IL-6 release from LPS-stimulated and unstimulated macrophages.
The objective of this study was to measure IL-6 release from LPS-stimulated and -unstimulated macrophages exposed to extracts from fresh and aged Scotchbond Multipurpose Plus adhesive disks (5 mm in diameter by 2 mm in thickness) light cured for 10, 20, or 40 s. One set of disks was aged for 16 weeks at 4 degrees C. Extracts were prepared by incubating three disks in 1 mL of serum-free culture medium for 72 h at 37 degrees C. Then macrophages (RAW 264.7) were exposed to the extracts (6.25-50 microL) for 72 h at 37 degrees C/5% CO(2). Supernatants were analyzed for cytokine levels (ELISA), and the monolayer of cells was assessed for viability (MTT assay). Unlike adhesive disk age, curing time affected cell viability. Disk extracts cured for 10 s were more cytotoxic (p < 0.05) than were extracts from 20- or 40-s cured disks. Macrophage release of IL-6 was stimulated significantly (p < 0.01) by extracts from fresh 10-s cured disks, up to 777 pg/mL and by 2 microg/mL of LPS (1174 pg/mL). The LPS response was significantly (p < 0.05) suppressed by 50 microL of extracts, which may be related to the enhanced cytotoxicity exhibited by LPS in combination with extracts. This study has demonstrated the possibility that IL-6 release is stimulated by light-cure dental adhesive applications using 10-s curings.
Kostoryz
Elisabet L
EL
Division of Pharmacology, School of Pharmacy, University of Missouri-Kansas City, 2411 Holmes Street, Kansas City, MO 64108-2792, USA. Kostoryze@umkc.edu
Eick
J David
JD
Chappelow
Cecil C
CC
Glaros
Alan G
AG
Wetmore
Lori
L
Yourtee
David M
DM
D000269:Adhesives / Q000528:radiation effects; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism; D003764:Dental Materials / Q000528:radiation effects; D004797:Enzyme-Linked Immunosorbent Assay; D015850:Interleukin-6 / Q000378:metabolism*; D008027:Light; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D051379:Mice; D013268:Stimulation, Chemical; D013778:Tetrazolium Salts; D013844:Thiazoles
D000269:Adhesives; D016207:Cytokines; D003764:Dental Materials; D015850:Interleukin-6; D008070:Lipopolysaccharides; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue