Ultrastructure of the central subnucleus of the nucleus tractus solitarii and the esophageal afferent terminals in the rat.
The central subnucleus of the nucleus tractus solitarii (ceNTS) receives afferent projections from the esophageal wall and projects to the nucleus ambiguus, thus serving as a relay nucleus for peristalsis of the esophagus. Here we examine the synaptic organization of the ceNTS, and its esophageal afferents by using transganglionic anterograde transport of cholera toxin-conjugated horseradish peroxidase (CT-HRP). When CT-HRP was injected into the subdiaphragmatic esophagus, many anterogradely labeled terminals were found only in the ceNTS. The ceNTS was composed of round or oval-shaped, small neurons (14.7x8.7 micro m) containing sparse organelles and an irregularly shaped nucleus. The average number of axosomatic terminals was only 1.3 per section cut through the nucleolus. Most of them (92%) contained round vesicles and formed asymmetric synaptic contacts (Gray's type I), and a few (8%) contained pleomorphic vesicles and formed symmetric synaptic contacts (Gray's type II). All anterogradely labeled terminals contacted dendrites but not the neuronal somata. The labeled terminals were large (2.55+/-0.07 micro m) and exclusively Gray's type I. More than half of them (60%) contacted small dendrites (less than 1 micro m in diameter), and contained dense-cored vesicles. More than 40% of the labeled terminals contacted two to four dendrites, thus forming a synaptic glomerulus. Sometimes a labeled terminal that contacted an unlabeled terminal by an adherent junction was found within the glomerulus. The large terminals and these complex synaptic relations appeared to characterize the esophageal afferent projections in the ceNTS.
Hayakawa
Tetsu
T
Department of Anatomy, Hyogo College of Medicine, Mukogawa, Nishinomiya, Hyogo 663-8501, Japan. tetsu@hyo-med.ac.jp
Takanaga
Akinori
A
Tanaka
Koichi
K
Maeda
Seishi
S
Seki
Makoto
M
D000344:Afferent Pathways / Q000502:physiology / Q000648:ultrastructure; D000818:Animals; D002772:Cholera Toxin / Q000008:administration & dosage / Q000378:metabolism; D004947:Esophagus / Q000294:innervation*; D006735:Horseradish Peroxidase / Q000378:metabolism; D007823:Laryngeal Nerves / Q000033:anatomy & histology / Q000502:physiology; D008297:Male; D008854:Microscopy, Electron; D009475:Neurons, Afferent / Q000502:physiology / Q000648:ultrastructure*; D010528:Peristalsis / Q000502:physiology; D017729:Presynaptic Terminals / Q000502:physiology / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017552:Solitary Nucleus / Q000502:physiology / Q000648:ultrastructure*
D002772:Cholera Toxin; D006735:Horseradish Peroxidase