title: [Serologic evidence for human Ehrlichiosis in Chile].
abstract: BACKGROUND
  Ehrlichiosis is a non contagious infectious disease, mainly transmitted by thick bites. In 1998, this infection was detected in dogs, for the first time, in Chile.
  
  
  OBJECTIVE
  To establish if there is human exposure to Ehrlichia sp in Chile.
  
  
  METHODS
  Blood samples from 17 dogs with ehrlichiosis and 19 humans who had contact with them were studied to determine human exposure to Ehrlichia equi and Ehrlichia chaffeensis in Chile. Samples were analyzed by indirect immunofluorecence and by polymerase chain reaction (PCR).
  
  
  RESULTS
  Six dogs had positive titers against both species of ehrlichia; 2 with titers of 1/256; 3 with titers over 1/512 to Ehrlichia equi and titers of 1/256, 1/128 and 1/64 to Ehrlichia chaffeensis respectively, and 1 with titers of 1/256 to Ehrlichia equi and titers of 1/128 to Ehrlichia chaffeensis. Two of the 19 humans, had positive titers against both antigens (1/128). PCR reactions were negative in both human and canine sera.
  
  
  CONCLUSIONS
  These results confirm that human exposure to Ehrlichia sp. Epidemiological surveillance for human ehrlichiosis should be implemented in the country.
authors.affiliation: Clinica Veterinaria Alcántara, Puente Alto, Santiago, Chile. javievet@entelchile.net
authors.forename: Javier
authors.initials: J
authors.lastname: López
authors.forename: Marisol
authors.initials: M
authors.lastname: Rivera
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Concha
authors.forename: Silvana
authors.initials: S
authors.lastname: Gatica
authors.forename: Mike
authors.initials: M
authors.lastname: Loeffeholz
authors.forename: Omar
authors.initials: O
authors.lastname: Barriga
mesh_terms: D000818:Animals; D002677:Chile; D004283:Dog Diseases / Q000097:blood* / Q000382:microbiology; D004285:Dogs; D041061:Ehrlichia canis / Q000276:immunology; D018469:Ehrlichia chaffeensis / Q000276:immunology; D016873:Ehrlichiosis / Q000097:blood* / Q000662:veterinary*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D016133:Polymerase Chain Reaction; D012698:Serologic Tests