title: Induction of glutathione-S-transferase in the Northern quahog Mercenaria mercenaria after exposure to the polychlorinated biphenyl (PCB) mixture Aroclor 1248.
abstract: The glutathione-S-transferases (GSTs) from the Northern quahog (Mercenaria mercenaria) were examined after an injection of a polychlorinated biphenyl (PCB) mixture, Aroclor 1248, to a concentration of approximately 50 ppm. Enzymatic analysis indicated a fourfold increase in the GST activity of quahogs injected with PCBs compared with that of the control. An electrophoretic analysis of the GST from the PCB-exposed quahogs showed a 1.5-fold increase in the concentration over that of the control. Purification of the GST on a glutathione affinity column yielded a glutathione binding protein, in addition to the GSTs. However, the amount of the glutathione binding protein in the PCB-injected quahogs was found to decrease by approximately 50% in comparison to the glutathione binding protein in the control quahogs.
authors.affiliation: Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth and The School for Marine Science and Technology, 285 Old Westport Road, North Dartmouth, Massachusetts 02747, USA.
authors.forename: Brian N
authors.initials: BN
authors.lastname: Blanchette
authors.forename: Bal Ram
authors.initials: BR
authors.lastname: Singh
mesh_terms: D000818:Animals; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D004785:Environmental Pollutants; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000378:metabolism; D007700:Kinetics; D008974:Mollusca / Q000187:drug effects* / Q000201:enzymology; D011078:Polychlorinated Biphenyls
chemical_list: D004785:Environmental Pollutants; D011078:Polychlorinated Biphenyls; D005982:Glutathione Transferase; D005978:Glutathione