[MRSA infection].
Molecular diagnosis of MRSA infection detecting mecA, spa gene and toxin gene by PCR including genotyping methods for MRSA nosocomial outbreak investigations are described. Restriction length polymorphism using pulsed-field gel electrophoresis(PFGE-RFLPs) with SmaI restriction enzyme is widely used for elucidating the infection route of MRSA. In Japan, MRSA is the major pathogen for nosocomial infections. These laboratory findings play an important role in infection control.
Mitsuda
Toshihiro
T
Division of Clinical Laboratory Medicine, Yokohama City University, School of Medicine.
D000942:Antigens, Bacterial / Q000032:analysis; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000032:analysis; D003428:Cross Infection / Q000175:diagnosis / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field; D004768:Enterotoxins / Q000032:analysis; D005838:Genotype; D006602:Hexosyltransferases; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D002267:Muramoylpentapeptide Carboxypeptidase / Q000032:analysis; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000235:genetics*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004768:Enterotoxins; D046915:Penicillin-Binding Proteins; C119204:streptococcal protective antigen; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase