New C-band protocol by heat denaturation in the presence of formamide.
C-banding techniques detect the presence of constitutive heterochromatin, which is usually located in centromeric regions of chromosomes in the majority of analysed species. The common method for C-banding used over the last 30 years involves treatment with a mild alkali barium hydroxide 5% Ba(OH)2 at 50 degrees C for 5-15 min and subsequent incubation in salt solution (2 x SSC at 60 degrees C for 1 h). We here present a new, easy and reliable technique for C-banding, which basically involves heat denaturation of chromosomal DNA in the presence of formamide and incubation in 2 x SSC at room temperature.
Fernández
R
R
Departamento de Psicobiologia, Facultad Ciencias de la Educación, Universidad de A Coruña, Coruña, Spain.
Barragán
M J L
MJ
Bullejos
M
M
Marchal
J A
JA
Díaz De La Guardia
R
R
Sanchez
A
A
D000818:Animals; D001483:Base Sequence; D002871:Chromosome Banding / Q000379:methods*; D017931:DNA Primers; D005559:Formamides / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D011489:Protein Denaturation; D012377:Rodentia / Q000235:genetics
D017931:DNA Primers; D005559:Formamides; C031066:formamide