title: Rescue of Hantaan virus minigenomes.
abstract: Hantavirus infections are a major public health concern worldwide. Their widespread geographical distribution and their ability to produce serious, often fatal, human disease underline the need for a system that allows manipulation of these viruses. We describe here the first successful establishment of a reverse genetics technology for Hantaan virus, the prototype of the genus Hantavirus. The system offers a unique opportunity to study the biology of hantaviruses, the pathogenesis of the diseases, and the efficacy of antiviral and prophylactic measures against hantavirus infections.
authors.affiliation: Special Pathogens Program, National Microbiology Laboratory, Health Canada, Canadian Science Centre for Human and Animal Health, 1015 Arlington Street, R3E 3R2, Winnipeg, Canada. Ramon_Flick@hc-sc.gc.ca
authors.forename: Kirsten
authors.initials: K
authors.lastname: Flick
authors.forename: Jay W
authors.initials: JW
authors.lastname: Hooper
authors.forename: Connie S
authors.initials: CS
authors.lastname: Schmaljohn
authors.forename: Ralf F
authors.initials: RF
authors.lastname: Pettersson
authors.forename: Heinz
authors.initials: H
authors.lastname: Feldmann
authors.forename: Ramon
authors.initials: R
authors.lastname: Flick
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D002522:Chlorocebus aethiops; D017930:Genes, Reporter; D005821:Genetic Techniques; D016679:Genome, Viral; D018063:Hantaan virus / Q000235:genetics* / Q000472:pathogenicity*; D018778:Hantavirus Infections / Q000209:etiology; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014709:Vero Cells
chemical_list: D012367:RNA, Viral; D015500:Chloramphenicol O-Acetyltransferase