Prospects of electron cryotomography to visualize macromolecular complexes inside cellular compartments: implications of crowding.
Electron cryotomography has unique potential for three-dimensional visualization of macromolecular complexes at work in their natural environment. This approach is based on reconstructing three-dimensional volumes from tilt series of electron micrographs of cells preserved in their native states by vitrification. Resolutions of 5-8 nm have already been achieved and the prospects for further improvement are good. Since many intracellular activities are conducted by complexes in the megadalton range with dimensions of 20-50 nm, current resolutions should suffice to identify many of them in tomograms. However, residual noise and the dense packing of cellular constituents hamper interpretation. Recently, tomographic data have been collected on vitrified eukaryotic cells (Medalia et al., Science (2002) in press). Their cytoplasm was found to be markedly less crowded than in the prokaryotes previously studied, in accord with differences in crowding between prokaryotic and eukaryotic cells documented by other (indirect) biophysical methods. The implications of this observation are twofold. First, complexes should be more easily identifiable in tomograms of eukaryotic cytoplasm. This applies both to recognizing known complexes and characterizing novel complexes. An example of the latter-a 5-fold symmetric particle is-given. Second, electron cryotomography offers an incisive probe to examine crowding in different cellular compartments.
Grünewald
Kay
K
Department of Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany.
Medalia
Ohad
O
Gross
Ariane
A
Steven
Alasdair C
AC
Baumeister
Wolfgang
W
D000818:Animals; D002477:Cells / Q000737:chemistry / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D006801:Humans; D007091:Image Processing, Computer-Assisted; D046911:Macromolecular Substances; D014054:Tomography
D046911:Macromolecular Substances