title: Determination of acrylamide in foods by GC/MS using 13C-labeled acrylamide as an internal standard.
abstract: A method was developed for the determination of trace amounts of acrylamide (AA) in foods. The method includes the addition of 13C-labeled acrylamide-1-13C (AA-1-13C) as an internal standard, extraction with water, bromination, clean-up with a Florisil cartridge column, dehydrobromination and GC/MS analysis in the selected ion monitoring (SIM) mode. Bromination of AA to 2,3-dibromopropionamide (2,3-DBPA) was done using potassium bromide and potassium bromate under an acidic condition. 2,3-DBPA was converted to 2-bromopropenamide (2-BPA) by dehydrobromination with triethylamine before GC/MS analysis. The recoveries of AA from spiked potato chips, corn snack, pretzel and roasted tea were 97-105%, and their relative standard deviations were 0.8-3.9%. The detection limit of AA in foods was 9 ng/g. The method was applied to thirty-one foods purchased from retail markets. AA was found in potato chips at the level of 466-3,340 ng/g, and in other foods at the level of ND-520 ng/g.
authors.affiliation: National Institute of Health Sciences: 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Nemoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takatsuki
authors.forename: Kumiko
authors.initials: K
authors.lastname: Sasaki
authors.forename: Tamio
authors.initials: T
authors.lastname: Maitani
mesh_terms: D020106:Acrylamide / Q000032:analysis*; D002247:Carbon Isotopes; D005504:Food Analysis / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*
chemical_list: D002247:Carbon Isotopes; D020106:Acrylamide