[Effect of the supernatant from mice liver cell primary culture on the in vitro growth of Plasmodium falciparum wild isolates].
Cultivation of Plasmodium falciparum has been a major research success, leading to a greater understanding of the parasite. Despite the fact that several P. falciparum clones have been maintained in continuous culture in different laboratories, research in genomics and proteomics would require parasitic material produced from fresh wild isolates. We have tested the effect of the supernatant from primary culture of mice hepatocytes on in vitro growth of P. falciparum isolates. Parasitized blood samples were collected from Madagascan malarious patients naturally infected. Isolates proliferation was assessed by use of isotopic method. The asexual erythrocytic stages of P. falciparum were grown for 42 hours in RPMI 1640-based medium plus L15 medium-based supernatant from mice liver cells culture, and in standard RPMI 1640-based medium alone. The mean of parasite growth was 1.5 times greater when the standard medium was enriched with the liver cells layer supernatant at a proportion of 10% and 15% (v/v). The usefulness of P. falciparum ex-vivo culture and of the hepatocytes in vitro primary culture is discussed.
Andrianantenaina
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HB
Institut Pasteur de Madagascar, Groupe de Recherche sur le Paludisme, BP 1274-1001 Antananarivo-Madagascar.
Randrianarivelojosia
M
M
Jambou
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R
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D017077:Culture Media, Conditioned / Q000592:standards*; D016895:Culture Media, Serum-Free / Q000592:standards; D023281:Genomics; D022781:Hepatocytes; D006801:Humans; D008270:Madagascar / Q000453:epidemiology; D016778:Malaria, Falciparum / Q000097:blood / Q000453:epidemiology / Q000469:parasitology; D051379:Mice; D009930:Organic Chemicals; D010963:Plasmodium falciparum / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification; D040901:Proteomics; D013997:Time Factors
D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; C095240:L15 culture medium; D009930:Organic Chemicals