Heme oxygenase-1 induction and regulation in unstimulated mouse peritoneal macrophages.
Heme oxygenase-1 (HO-1) is a stress protein induced by a variety of stimuli in inflammatory cells. This study was set up to investigate the induction of this protein in unstimulated macrophages. Resident mouse peritoneal macrophages purified by adhesion and cultured in basal conditions strongly induced HO-1 in a time-dependent manner, with a peak at 20 hr. At the same time, low levels of nitrite accumulated in the culture medium and expression of nitric oxide synthase-2 (NOS-2) and NOS-3 protein was detected. Inhibition of NO production and/or NOS expression by incubating macrophages with different drugs inhibiting NOS activity or modulating the redox state of the cell, such as N-acetylcysteine (NAC) resulted in inhibition of HO-1 expression, suggesting that NO is an endogenous mediator of this stress response. In conclusion, mouse peritoneal macrophages cultured in basal conditions develop an adaptive response with up-regulation of HO-1 as a very sensitive marker of oxidative stress.
Vicente
Ana María
AM
Department of Pharmacology, University of Valencia, Burjasot, Spain.
Guillén
María Isabel
MI
Alcaraz
María José
MJ
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D004790:Enzyme Induction; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis*; D051547:Heme Oxygenase-1; D007150:Immunohistochemistry; D017737:Macrophages, Peritoneal / Q000201:enzymology* / Q000378:metabolism; D008565:Membrane Proteins; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010084:Oxidation-Reduction
D008565:Membrane Proteins; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse