Rescue of Hantaan virus minigenomes.
Hantavirus infections are a major public health concern worldwide. Their widespread geographical distribution and their ability to produce serious, often fatal, human disease underline the need for a system that allows manipulation of these viruses. We describe here the first successful establishment of a reverse genetics technology for Hantaan virus, the prototype of the genus Hantavirus. The system offers a unique opportunity to study the biology of hantaviruses, the pathogenesis of the diseases, and the efficacy of antiviral and prophylactic measures against hantavirus infections.
Flick
Kirsten
K
Special Pathogens Program, National Microbiology Laboratory, Health Canada, Canadian Science Centre for Human and Animal Health, 1015 Arlington Street, R3E 3R2, Winnipeg, Canada. Ramon_Flick@hc-sc.gc.ca
Hooper
Jay W
JW
Schmaljohn
Connie S
CS
Pettersson
Ralf F
RF
Feldmann
Heinz
H
Flick
Ramon
R
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D002522:Chlorocebus aethiops; D017930:Genes, Reporter; D005821:Genetic Techniques; D016679:Genome, Viral; D018063:Hantaan virus / Q000235:genetics* / Q000472:pathogenicity*; D018778:Hantavirus Infections / Q000209:etiology; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014709:Vero Cells
D012367:RNA, Viral; D015500:Chloramphenicol O-Acetyltransferase