title: Lack of biofilm contribution to bacterial colonisation in an experimental model of foreign body infection by Staphylococcus aureus and Staphylococcus epidermidis.
abstract: The contribution of in vivo biofilm-forming potential of Staphylococcus aureus and Staphylococcus epidermidis was studied in an experimental model of foreign body infections. Increasing inocula (from 10(2) to 10(7) organisms) of ica-positive strains of S. aureus and S. epidermidis and their ica-negative isogenic mutants (the ica locus codes for a major polysaccharide component of biofilm) were injected into subcutaneously implanted tissue cages in guinea pigs. Surprisingly, bacterial counts and time-course of tissue cage infection by ica-positive strains of S. aureus or S. epidermidis were equivalent to those of their respective ica-negative mutants, in the locally infected fluids and on tissue-cage-inserted plastic coverslips.
authors.affiliation: Division of Infectious Diseases, University Hospitals of Geneva, 1211 Geneva 14, Switzerland. patrice.francois@hcuge.ch
authors.forename: Patrice
authors.initials: P
authors.lastname: Francois
authors.forename: Patrick H
authors.initials: PH
authors.lastname: Tu Quoc
authors.forename: Carmelo
authors.initials: C
authors.lastname: Bisognano
authors.forename: William L
authors.initials: WL
authors.lastname: Kelley
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Lew
authors.forename: Jacques
authors.initials: J
authors.lastname: Schrenzel
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Cramton
authors.forename: Friedrich
authors.initials: F
authors.lastname: Götz
authors.forename: Pierre
authors.initials: P
authors.lastname: Vaudaux
mesh_terms: D000818:Animals; D018441:Biofilms; D004195:Disease Models, Animal; D005547:Foreign Bodies / Q000150:complications*; D006168:Guinea Pigs; D013203:Staphylococcal Infections / Q000209:etiology*; D013211:Staphylococcus aureus / Q000472:pathogenicity*; D013212:Staphylococcus epidermidis / Q000472:pathogenicity*