Specific SR protein-dependent splicing substrates identified through genomic SELEX.
The Drosophila pre-mRNA splicing factor B52 (SRp55) is essential for fly development, but splicing of RNAs of specific genes tested previously is normal in B52-null animals, presumably due to partial functional redundancy with other SR proteins. To identify B52-dependent splicing substrates in vivo, we selected genomic sequence fragments whose transcripts bind B52. Almost all of the corresponding genes having a known function encode either transcription factors or components of signal transduction pathways, with the B52- binding fragments located to not only exonic but also intronic regions. Some pre-mRNAs from these genes showed splicing defects in the B52-null mutant. These results indicate that B52 has unique functions in the removal of some introns during development, and plays a critical role in cellular regulatory networks.
Kim
Soyoun
S
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Shi
Hua
H
Lee
Dong-Ki
DK
Lis
John T
JT
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D024202:Electrophoretic Mobility Shift Assay; D016678:Genome; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D009843:Oligoribonucleotides / Q000235:genetics / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D012326:RNA Splicing / Q000235:genetics*; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism
D029721:Drosophila Proteins; D009687:Nuclear Proteins; D009843:Oligoribonucleotides; D010750:Phosphoproteins; D012322:RNA Precursors; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins; C070791:B52 protein, Drosophila