title: [Effects of polyamine-loaded liposomes on liver mitochondrial permeability transition].
abstract: The objective of the present study was represented by the effects of polyamineloaded liposomes on hepatic mitochondrial permeability transition (MPT). MPT was appreciated through the swelling of the isolated guinea-pig liver mithocondria induced by Ca2+, at 540 nm, using a diode-array 8452 UV-VIS spectrophotometer and a General Purpose software (Hewlett-Packard). Polyamines encapsulated in liposomes might inhibit the appearance of MPT induced by Ca2+, directly proportional to electrical charge: spermine > spermidine > putrescine > cadaverine. Thus, together with some previous data, it was observed that spermine may modulate hepatic MPT from the exterior, as well as from the interior of mitochondria. Moreover, spermidine and putrescine may also modulate MPT from the interior of mitochondria at low doses, and from the exterior only at high doses. The changes in mitochondrial membranes lipid composition (as done by control liposomes) are slightly influencing the MPT. The increase in membrane rigidity through the use of 40% cholesterol-enriched liposomes is drastically decreasing the appearance and development of MPT.
authors.affiliation: Facultatea de Medicină Stomatologică, Universitatea de Medicină şi Farmacie Gr.T. Popa Iaşi.
authors.forename: M
authors.initials: M
authors.lastname: Costuleanu
authors.forename: Liliana
authors.initials: L
authors.lastname: Foia
authors.forename: Natalia
authors.initials: N
authors.lastname: Costuleanu
authors.forename: Anca
authors.initials: A
authors.lastname: Indrei
authors.forename: Simona Mihaela
authors.initials: SM
authors.lastname: Slătineanu
authors.forename: Gh
authors.initials: G
authors.lastname: Petrescu
mesh_terms: D000818:Animals; D015317:Biogenic Polyamines / Q000493:pharmacokinetics*; D002463:Cell Membrane Permeability / Q000502:physiology*; D003196:Computer Graphics; D006168:Guinea Pigs; D008081:Liposomes / Q000493:pharmacokinetics*; D008930:Mitochondria, Liver / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D013053:Spectrophotometry; D013096:Spermine / Q000378:metabolism
chemical_list: D015317:Biogenic Polyamines; D008081:Liposomes; D013096:Spermine; D011700:Putrescine