title: Heparan sulphate N-sulphotransferase activity: reaction mechanism and substrate recognition.
abstract: Human heparan sulphate N-deacetylase/N-sulphotransferase 1 sulphates the NH(3) (+) group of the glucosamine moiety of the heparan chain in heparan sulphate/heparin biosynthesis. An open cleft that runs perpendicular to the sulphate donor 3'-phosphoadenosine 5'-phosphosulphate may constitute the acceptor substrate-binding site of the sulphotransferase domain (hNST1) [Kakuta, Sueyoshi, Negishi and Pedersen (1999) J. Biol. Chem. 274, 10673-10676]. When a hexasaccharide model chain is docked into the active site, only a trisaccharide (-IdoA-GlcN-IdoA-) portion interacts directly with the cleft residues: Trp-713, His-716 and His-720 from alpha helix 6, and Phe-640, Glu-641, Glu-642, Gln-644 and Asn-647 from random coil (residues 640-647). Mutation of these residues either abolishes or greatly reduces hNST1 activity. Glu-642 may play the critical role of catalytic base in the sulphuryl group transfer reaction, as indicated by its hydrogen-bonding distance to the NH(3) (+) group of the glucosamine moiety in the model and by mutational data.
authors.affiliation: Pharmacogenetics Section, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, U.S.A.
authors.forename: Y
authors.initials: Y
authors.lastname: Kakuta
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: L C
authors.initials: LC
authors.lastname: Pedersen
authors.forename: L G
authors.initials: LG
authors.lastname: Pedersen
authors.forename: M
authors.initials: M
authors.lastname: Negishi
mesh_terms: D000818:Animals; D001665:Binding Sites; D006801:Humans; D008958:Models, Molecular; D009154:Mutation; D011485:Protein Binding; D013379:Substrate Specificity; D015238:Sulfotransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D015238:Sulfotransferases; C021350:heparitin sulfotransferase