Tropane alkaloids in adventitious root cultures of Physochlaina physaloides.
Adventitious root cultures of Physochlaina physaloides were established and their growth and tropane alkaloid production were investigated. Although the roots cultured in Murashige-skoog liquid medium containing 1.0-3.0 mg/l IBA grew well, the addition of lower concentrations of auxins was more suitable for alkaloid production. The best alkaloid yield was obtained with 0.01 mg/l NAA in 1/2 MS liquid medium. In addition, the alkaloid differences between the cultured roots and the regenerated plant cultivated in the soil was studied. Cultured roots in this condition showed higher level of alkaloids when compared to leaf, stem and roots from the regenerated plant (3 months in field conditions).
Shimomura
Koichiro
K
Faculty of Life Science, Toyo University, 1-1-1 Izumino, Itakura-machi, Oura-gun, Gunma, 374-0193 Japan.
Hirose
Makoto
M
Natori
Shinsaku
S
Satake
Motoyoshi
M
Yoshimatsu
Kayo
K
Ishimaru
Kanji
K
D003470:Culture Media; D007210:Indoleacetic Acids / Q000494:pharmacology; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D012987:Soil; D019657:Solanaceae / Q000254:growth & development / Q000378:metabolism*; D014326:Tropanes / Q000378:metabolism*
D003470:Culture Media; D007210:Indoleacetic Acids; D012987:Soil; D014326:Tropanes