title: Periparturient endocrine and metabolic changes in healthy cows and in cows affected by mastitis.
abstract: Transition from pregnancy to lactation in dairy cows involves considerable metabolic adaptation. Additional stress is incurred during infections such as periparturient mastitis. Multiparous Holstein-Friesian cows kept under normal production conditions (n = 15) were used to evaluate changes in circulating metabolite and hormone concentrations from 5 days before to 5 days after calving. Insulin-like growth factor binding protein (IGFBP) profiles were also monitored. Marked time-related changes were observed for plasma thyroid hormone, IGF, cortisol, insulin, beta-hydroxybutyrate (BHB) and non-esterified fatty acid (NEFA) concentrations but not for plasma leptin. A decrease in IGF-II concentration and maximal intensity of the putative IGFBP-1 band occurred at parturition. When compared with the five healthy cows,low IGF-II levels were prolonged to day 2 post-partum in five cows with Escherichia coli-associated mastitis. However, marked decreases in IGFBP-2 band intensity were evident only in two of the four cases examined. Individual total ligand (IGF-I + IGF-II) concentration and IGFBP pattern prepartum were largely regained 5 days post-partum in all cows. Hormone and metabolite concentrations in the two cows with Staphylococcus aureus-associated mastitis were very similar to those in the five healthy cows. Plasma thyroxine (T4) was lower 2 days prepartum in the cows, which later developed Gram-negative mastitis. Multiregression analysis showed that variance in T4 concentration was significantly and independently associated with triiodothyronine (T3) and IGF-I positively and with cortisol negatively (R2 = 0.648). This study confirms the close inter-relationship between the thyroid hormone and IGF axes in cattle and indicates possible effects of Gram-negative mastitis infection on IGF-II metabolism.
authors.affiliation: INEP - Institute for the Application of Nuclear Energy, Zemun, Yugoslavia. anikolic@inep.co.yu
authors.forename: J A
authors.initials: JA
authors.lastname: Nikolić
authors.forename: M
authors.initials: M
authors.lastname: Kulcsár
authors.forename: L
authors.initials: L
authors.lastname: Kátai
authors.forename: O
authors.initials: O
authors.lastname: Nedić
authors.forename: Sz
authors.initials: S
authors.lastname: Jánosi
authors.forename: Gy
authors.initials: G
authors.lastname: Huszenicza
mesh_terms: D000818:Animals; D002417:Cattle / Q000378:metabolism*; D004927:Escherichia coli Infections / Q000378:metabolism; D005260:Female; D006854:Hydrocortisone / Q000097:blood; D007328:Insulin / Q000097:blood; D018969:Insulin-Like Growth Factor Binding Proteins / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000378:metabolism; D007774:Lactation / Q000378:metabolism*; D020738:Leptin / Q000097:blood; D008414:Mastitis, Bovine / Q000378:metabolism*; D049590:Postpartum Period / Q000378:metabolism*; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood
chemical_list: D007328:Insulin; D018969:Insulin-Like Growth Factor Binding Proteins; D020738:Leptin; D014284:Triiodothyronine; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D013974:Thyroxine; D006854:Hydrocortisone