Applications of plasmid electrotransfer.
The use of electric pulses to transfect cells has recently been extended to show the utility of this procedure in vivo. Electrotransfer has been performed in vivo on several tissue types including skin, blood vessels, liver, tumor, muscle, cornea, brain and spleen. The most widely targeted tissue has been skeletal muscle. In addition to its potential use in gene therapy, in vivo DNA electrotransfer is also, because of its simplicity, a powerful laboratory tool to study in vivo gene expression and function in a given tissue. Many published studies have now shown that plasmid electrotransfer can lead to a long-lasting therapeutic effect in various pathologies, such as cancer, blood disease, or muscle ischemia. The future potential for this gene therapy approach will include delivery for both local action or distal effect by secretion of the transgenic proteins in the circulation.
Scherman
Daniel
D
Laboratoire de Chimie Bioorganique, UMR 7001 ENSCP/CNRS/Gencell SA, CRVA-Aventis, Batiment Monod. 13 quai Jules Guesde, 94403 Vitry-sur-Seine cedex, France. Daniel.scherman@aventis.com
Bigey
Pascal
P
Bureau
Michel Francis
MF
D000818:Animals; D030801:Animals, Genetically Modified; D004247:DNA / Q000378:metabolism; D018274:Electroporation / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D018482:Muscle, Skeletal / Q000378:metabolism; D009369:Neoplasms / Q000628:therapy; D010957:Plasmids / Q000378:metabolism; D014162:Transfection
D004247:DNA