Involvement of caveolin-2 in caveolar biogenesis in MDCK cells.
Caveolins have been identified as key components of caveolae, specialized cholesterol-enriched raft domains visible as small flask-shaped invaginations of the plasma membrane. In polarized MDCK cells caveolin-1 and -2 are found together on basolateral caveolae whereas the apical membrane, where only caveolin-1 is present, lacks caveolae. Expression of a caveolin mutant prevented the formation of the large caveolin-1/-2 hetero-oligomeric complexes, and led to intracellular retention of caveolin-2 and disappearance of caveolae from the basolateral membrane. Correspondingly, in MDCK cells over-expressing caveolin-2 the basolateral membrane exhibited an increased number of caveolae. These results indicate the involvement of caveolin-2 in caveolar biogenesis.
Lahtinen
Ulla
U
Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
Honsho
Masanori
M
Parton
Robert G
RG
Simons
Kai
K
Verkade
Paul
P
D000818:Animals; D021941:Caveolae / Q000502:physiology* / Q000648:ultrastructure; D051243:Caveolin 2; D022461:Caveolins / Q000502:physiology*; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron
D051243:Caveolin 2; D022461:Caveolins