title: The C-type lectin DC-SIGN (CD209) is an antigen-uptake receptor for Candida albicans on dendritic cells.
abstract: Dendritic cells (DC) that express the type II C-type lectin DC-SIGN (CD209) are located in the submucosa of tissues, where they mediate HIV-1 entry. Interestingly, the pathogen Candida albicans, the major cause of hospital-acquired fungal infections, penetrates at similar submucosal sites. Here we demonstrate that DC-SIGN is able to bind C. albicans both in DC-SIGN-transfected cell lines and in human monocyte-derived DC. The binding was shown to be time- as well as concentration-dependent, and live as well as heat-inactivated C. albicans were bound to the same extent. Moreover, in immature DC, DC-SIGN was able to internalize C. albicans in specific DC-SIGN-enriched vesicles, distinct from those containing the mannose receptor, the other known C. albicans receptor expressed by DC. Together, these results demonstrate that DC-SIGN is an exquisite pathogen-uptake receptor that captures not only viruses but also fungi.
authors.affiliation: Department of Tumor Immunology, Nijmegen Center for Molecular Life Sciences, University Medical Center Nijmegen, Nijmegen, The Netherlands.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Cambi
authors.forename: Karlijn
authors.initials: K
authors.lastname: Gijzen
authors.forename: l Jolanda M
authors.initials: lJ
authors.lastname: de Vries
authors.forename: Ruurd
authors.initials: R
authors.lastname: Torensma
authors.forename: Ben
authors.initials: B
authors.lastname: Joosten
authors.forename: Gosse J
authors.initials: GJ
authors.lastname: Adema
authors.forename: Mihai G
authors.initials: MG
authors.lastname: Netea
authors.forename: Bart-Jan
authors.initials: BJ
authors.lastname: Kullberg
authors.forename: Luigina
authors.initials: L
authors.lastname: Romani
authors.forename: Carl G
authors.initials: CG
authors.lastname: Figdor
mesh_terms: D020410:Activated-Leukocyte Cell Adhesion Molecule / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000276:immunology*; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000276:immunology* / Q000382:microbiology; D004533:Egtazic Acid / Q000494:pharmacology; D004705:Endocytosis; D011992:Endosomes; D006358:Hot Temperature; D006801:Humans; D020014:K562 Cells; D037181:Lectins, C-Type / Q000276:immunology*; D009000:Monocytes / Q000166:cytology; D010587:Phagocytosis / Q000187:drug effects; D010588:Phagosomes / Q000382:microbiology; D011956:Receptors, Cell Surface / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014162:Transfection; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D020410:Activated-Leukocyte Cell Adhesion Molecule; D000946:Antigens, Fungal; D015815:Cell Adhesion Molecules; D004533:Egtazic Acid; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin