The C-type lectin DC-SIGN (CD209) is an antigen-uptake receptor for Candida albicans on dendritic cells.
Dendritic cells (DC) that express the type II C-type lectin DC-SIGN (CD209) are located in the submucosa of tissues, where they mediate HIV-1 entry. Interestingly, the pathogen Candida albicans, the major cause of hospital-acquired fungal infections, penetrates at similar submucosal sites. Here we demonstrate that DC-SIGN is able to bind C. albicans both in DC-SIGN-transfected cell lines and in human monocyte-derived DC. The binding was shown to be time- as well as concentration-dependent, and live as well as heat-inactivated C. albicans were bound to the same extent. Moreover, in immature DC, DC-SIGN was able to internalize C. albicans in specific DC-SIGN-enriched vesicles, distinct from those containing the mannose receptor, the other known C. albicans receptor expressed by DC. Together, these results demonstrate that DC-SIGN is an exquisite pathogen-uptake receptor that captures not only viruses but also fungi.
Cambi
Alessandra
A
Department of Tumor Immunology, Nijmegen Center for Molecular Life Sciences, University Medical Center Nijmegen, Nijmegen, The Netherlands.
Gijzen
Karlijn
K
de Vries
l Jolanda M
lJ
Torensma
Ruurd
R
Joosten
Ben
B
Adema
Gosse J
GJ
Netea
Mihai G
MG
Kullberg
Bart-Jan
BJ
Romani
Luigina
L
Figdor
Carl G
CG
D020410:Activated-Leukocyte Cell Adhesion Molecule / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000276:immunology*; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000276:immunology* / Q000382:microbiology; D004533:Egtazic Acid / Q000494:pharmacology; D004705:Endocytosis; D011992:Endosomes; D006358:Hot Temperature; D006801:Humans; D020014:K562 Cells; D037181:Lectins, C-Type / Q000276:immunology*; D009000:Monocytes / Q000166:cytology; D010587:Phagocytosis / Q000187:drug effects; D010588:Phagosomes / Q000382:microbiology; D011956:Receptors, Cell Surface / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014162:Transfection; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D020410:Activated-Leukocyte Cell Adhesion Molecule; D000946:Antigens, Fungal; D015815:Cell Adhesion Molecules; D004533:Egtazic Acid; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin