Cloning and sequencing of the Thermus aquaticus glycerol facilitator gene.
The gene glpK, encoding glycerol kinase of Thermus aquaticus has been identified [Biosci. Biotechnol. Biochem., 62 (1998) 2375-2381]. In the present work, the nucleotide sequence of glpFK operon and the gene glpF encoding glycerol facilitator were determined. T. aquaticus GlpF was predicted to contain 272 amino acids with six putative transmembrane segments and two half-membrane-spanning segments that contained the motif Asn-Pro-Ala, respectively. The amino acid residues involved in the discrimination of glycerol were deduced to be Trp44, Tyr182, and Arg188.
Huang
Hua-Shan
HS
School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852, Japan.
Ito
Kiyoshi
K
Yoshimoto
Tadashi
T
D000595:Amino Acid Sequence; D020346:Aquaporins / Q000235:genetics; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics; D005991:Glycerol Kinase / Q000235:genetics*; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D013824:Thermus / Q000235:genetics*
D020346:Aquaporins; D029968:Escherichia coli Proteins; C078043:GlpF protein, E coli; D005991:Glycerol Kinase