Secreted adhesion molecules of Strongyloides venezuelensis are produced by oesophageal glands and are components of the wall of tunnels constructed by adult worms in the host intestinal mucosa.
The parasitic female of Strongyloides venezuelensis keeps invading the epithelial layer of the host intestinal mucosa. Upon invasion, it adheres to the surface of the intestinal epithelial cells with adhesion molecules secreted from the mouth. It has been demonstrated that S. venezuelensis are expelled from the intestine because mucosal mast cells inhibit the attachment of adult worms to the mucosal surface. In the present study, we generated specific antibodies against secreted adhesion molecules to investigate their function in vivo, because these molecules have been demonstrated only in vitro in spite of the importance in the infection processes. A mouse monoclonal antibody specific to S. venezuelensis adhesion molecules inhibited the attachment of adult worms to plastic dishes and the binding of adhesion molecules to rat intestinal epithelial cells. Immunohistochemical study revealed that adhesion molecules were produced by oesophageal glands and were continuously secreted in vivo to line the wall of the tunnels formed by adult worms in the intestinal mucosa. Our findings indicate that adhesion molecules play essential roles in the infection processes of S. venezuelensis in the host intestine.
Maruyama
H
H
Department of Molecular Parasitology, Nagoya City University Graduate School of Medical Sciences, Kawasumi, Mizuho-cho, Mizuho, Nagoya 467-8601, Japan. maruyama@med.nagoya-cu.ac.jp
El-Malky
M
M
Kumagai
T
T
Ohta
N
N
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002352:Carrier Proteins / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D004947:Esophagus / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism; D005849:Gerbillinae; D006790:Host-Parasite Interactions; D007114:Immunization; D007413:Intestinal Mucosa / Q000469:parasitology*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D051381:Rats; D013321:Strongyloides / Q000033:anatomy & histology / Q000378:metabolism / Q000472:pathogenicity*; D013322:Strongyloidiasis / Q000469:parasitology*
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins