title: Electrophysiological characterization of cardiac muscarinic acetylcholine receptors: different subtypes mediate different potassium currents.
abstract: To characterize electrophysiologically the K+ currents mediated by various mAChR subtypes, we performed detailed whole-cell patch-clamp studies in canine atrial myocytes. I(KACh) was induced by 1 mM ACh (acetylcholine) or by arecaidine but-2-ynyl ester tosylate (100 nM, an M2 receptor selective agonist) and was blocked by methoctramine (20 nM, an M2 receptor selective antagonist). Tetramethylammonium (0.5 mM) activated a K+ conductance with delayed rectifying properties (I(KM3)) and the currents were highly sensitive to 4-diphenylacetoxy-N-methylpiperidine methiodide (2 nM, an M3 receptor inhibitor). 4-aminopyridine (1 mM) induced a delayed rectifier-like current (I(K4AP)) which was selectively suppressed by tropicamide (200 nM, an M4 receptor blocker). The current waveforms, I-V relationships, steady-state voltage-dependence, kinetics and pharmacological properties of these three currents were different from one another and distinct from the classical delayed rectifier K+ currents (I(Kr) and I(Ks)). Both I(KACh) and I(K4AP) were sensitive to pertussis ntoxin, whereas I(KM3) was not. Isoproterenol (1 mM) markedly depressed I(KM3), but increased I(K4AP) and did not alter I(KACh). The effects of isoproterenol were reversed by propranolol (1 mM); and ACh completely suppressed I(KM3) and I(K4AP). The results suggest that the K+ currents mediated by different subtypes of mAChR represent different populations of K+ channels and that the cholinergic regulation of the heart's electrical function is a consequence of activating multiple mAChRs linked to different effector systems with potentially varying signal transduction.
authors.affiliation: Research Center, Montreal Heart Institute, Montreal, Quebec, Canada.
authors.forename: Hong
authors.initials: H
authors.lastname: Shi
authors.forename: Baofeng
authors.initials: B
authors.lastname: Yang
authors.forename: Donghui
authors.initials: D
authors.lastname: Xu
authors.forename: Huizhen
authors.initials: H
authors.lastname: Wang
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D004285:Dogs; D004553:Electric Conductivity; D004594:Electrophysiology; D005260:Female; D006325:Heart Atria / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000187:drug effects; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000378:metabolism*; D021122:Protein Subunits; D011976:Receptors, Muscarinic / Q000378:metabolism*; D013997:Time Factors
chemical_list: D015221:Potassium Channels; D021122:Protein Subunits; D011976:Receptors, Muscarinic; D011188:Potassium