title: Ribosome-inactivating proteins depurinate poly(ADP-ribosyl)ated poly(ADP-ribose) polymerase and have transforming activity for 3T3 fibroblasts.
abstract: It has been known that ribosome-inactivating proteins (RIPs) from plants damage ribosomes by removing adenine from a precise position of rRNA. Subsequently it was observed that all tested RIPs depurinate DNA, and some of them also non-ribosomal RNAs and poly(A), hence the denomination of adenine polynucleotide glycosylases was proposed. We report now that ricin, saporin-L2, saporin-S6, gelonin and momordin depurinate also poly(ADP-ribosyl)ated poly(ADP-ribose) polymerase (auto modified enzyme), an enzyme involved in DNA repair. We observed also that all RIPs but gelonin induce transformation of fibroblasts, possibly as a consequence of damage to DNA and of the altered DNA repair system.
authors.affiliation: Dipartimento di Patologia sperimentale, Università di Bologna, Via San Giacomo 14, I-40126 Bologna, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Barbieri
authors.forename: Maurizio
authors.initials: M
authors.lastname: Brigotti
authors.forename: Paolo
authors.initials: P
authors.lastname: Perocco
authors.forename: Domenica
authors.initials: D
authors.lastname: Carnicelli
authors.forename: Marialibera
authors.initials: M
authors.lastname: Ciani
authors.forename: Laura
authors.initials: L
authors.lastname: Mercatali
authors.forename: Fiorenzo
authors.initials: F
authors.lastname: Stirpe
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002461:Cell Line, Transformed; D051379:Mice; D008807:Mice, Inbred BALB C; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D011506:Proteins / Q000502:physiology*; D011687:Purines / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D011506:Proteins; D011687:Purines; D011065:Poly(ADP-ribose) Polymerases