Development of a 5'-nuclease PCR assay for detecting Shiga toxin-producing Escherichia coli O145 based on the identification of an 'O-island 29' homologue.
OBJECTIVE
A DNA sequence, from Escherichia coli STEC O145, homologous to O-island 29 from STEC O157 is described, together with a real-time PCR assay for detecting it.


RESULTS
PCR and sequencing were used to identify the 'O-island 29' homologous DNA sequence from STEC O145 (strain VTH34). The sequence divergence between the STEC O145 and O157 'O-island 29' allowed a STEC O145 5'-nuclease PCR assay to be developed.


CONCLUSIONS
The characterization of a novel locus in STEC O145 has allowed a specific O145 serogroup 5'-nuclease PCR assay to be designed.


CONCLUSIONS
These findings increase the number of serogroup PCR assays available as alternatives to classical O-serotyping of E. coli.
Perelle
S
S
Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches sur l'Hygiène et la Qualité des Aliments, Unité: Atelier de Biotechnologie, Maisons-Alfort, France. s.perelle@afssa.fr
Dilasser
F
F
Grout
J
J
Fach
P
P
D000595:Amino Acid Sequence; D015373:Bacterial Typing Techniques / Q000379:methods*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D019453:Escherichia coli O157 / Q000145:classification* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D022621:Shiga Toxin / Q000096:biosynthesis* / Q000235:genetics
D004269:DNA, Bacterial; D022621:Shiga Toxin