title: Flagella are virulence determinants of Burkholderia pseudomallei.
abstract: Burkholderia pseudomallei, a facultatively intracellular pathogen, is a flagellated and motile gram-negative bacterium and is the causative agent of melioidosis in humans. Flagella are commonly recognized as important virulence determinants expressed by bacterial pathogens since the motility phenotype imparted by these organelles often correlates with the ability of an organism to cause disease. We used a virulent isolate of B. pseudomallei, KHW, to construct an isogenic deletion mutant with a mutation in the flagellin gene (fliC) by gene replacement transposon mutagenesis. The KHWDeltafliCKm mutant was aflagellate and nonmotile in semisolid agar. The isogenic KHWDeltafliCKm mutant was not impaired in terms of the ability to invade and replicate in cultured human lung cells compared with the wild type. It was also equally virulent in slow-killing assays involving Caenorhabditis elegans, but it was avirulent during intranasal infection of BALB/c mice. Very few bacteria, if any, were isolated from the lungs and spleens of KHWDeltafliCKm-infected mice. In contrast, the bacterial loads in the lungs and spleens were similar in mice infected with KHW and in mice infected with the complemented mutant, KHWDeltafliCKm/pUCP28TfliC. Unlike the Syrian hamster or diabetic rat models of infection, the B. pseudomallei flagellin was also a virulence factor during intraperitoneal infection of BALB/c mice. In this study, all animals infected with KHWDeltafliCKm remained healthy and did not succumb to disease regardless of the route of infection. The flagellum is therefore an important and necessary virulence determinant of B. pseudomallei during intranasal and intraperitoneal infection of mice.
authors.affiliation: Department of Biochemistry, National University of Singapore, Faculty of Medicine, 10 Kent Ridge Crescent, Singapore 119260. bchckl@nus.edu.sg
authors.forename: K L
authors.initials: KL
authors.lastname: Chua
authors.forename: Y Y
authors.initials: YY
authors.lastname: Chan
authors.forename: Y H
authors.initials: YH
authors.lastname: Gan
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D016957:Burkholderia pseudomallei / Q000235:genetics / Q000472:pathogenicity*; D017173:Caenorhabditis elegans / Q000382:microbiology; D005407:Flagella / Q000502:physiology*; D005408:Flagellin / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008554:Melioidosis / Q000382:microbiology / Q000503:physiopathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D014407:Tumor Cells, Cultured; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D005408:Flagellin