The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre.
Yeast Erv1p is a ubiquitous FAD-dependent sulfhydryl oxidase, located in the intermembrane space of mitochondria. The dimeric enzyme is essential for survival of the cell. Besides the redox-active CXXC motif close to the FAD, Erv1p harbours two additional cysteine pairs. Site-directed mutagenesis has identified all three cysteine pairs as essential for normal function. The C-terminal cysteine pair is of structural importance as it contributes to the correct arrangement of the FAD-binding fold. Variations in dimer formation and unique colour changes of mutant proteins argue in favour of an interaction between the N-terminal cysteine pair with the redox centre of the partner monomer.
Hofhaus
Götz
G
Institut für Biochemie und Biologisch-Medizinisches Forschungszentrum, Heinrich-Heine-Universität Düsseldorf, Germany. hofhaus@uni-duesseldorf.de
Lee
Jeung-Eun
JE
Tews
Ivo
I
Rosenberg
Beate
B
Lisowsky
Thomas
T
D001665:Binding Sites / Q000502:physiology; D003116:Color; D017124:Conserved Sequence; D003545:Cysteine / Q000378:metabolism; D019281:Dimerization; D004789:Enzyme Activation / Q000502:physiology; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005816:Genetic Complementation Test; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D050862:Oxidoreductases Acting on Sulfur Group Donors; D017434:Protein Structure, Tertiary / Q000502:physiology; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D013053:Spectrophotometry; D013329:Structure-Activity Relationship
D003545:Cysteine; D005182:Flavin-Adenine Dinucleotide; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D050862:Oxidoreductases Acting on Sulfur Group Donors; D029701:Saccharomyces cerevisiae Proteins; D012694:Serine; C111280:ERV2 protein, S cerevisiae; C073790:ERV1 protein, S cerevisiae