title: Amplifying whole insect genomes with multiple displacement amplification.
abstract: Many applications in insect genetics require repeated analyses on individual organisms. These include population genetic and genomics, linkage mapping, molecular systematics and map-based positional cloning. However, using the polymerase chain reaction, a limited number of analyses are possible with DNA isolated from whole bodies or parts of small or preserved specimens. We describe the optimization of a new technique, Multiple Displacement Amplification, for 100-400-fold amplification of whole mosquito genomes. We demonstrate that MDA amplifies genomic DNA directly from an adult leg or from organisms as small as a first instar mosquito larva. Genetic polymorphisms revealed at individual loci are the same whether using the original genomic DNA or MDA DNA as a template.
authors.affiliation: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA. normago@lamar.colostate.edu
authors.forename: N
authors.initials: N
authors.lastname: Gorrochotegui-Escalante
authors.forename: W C
authors.initials: WC
authors.lastname: Black
mesh_terms: D000330:Aedes / Q000235:genetics*; D000818:Animals; D004247:DNA / Q000032:analysis / Q000235:genetics; D005260:Female; D017344:Genes, Insect / Q000235:genetics*; D005821:Genetic Techniques; D016678:Genome; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: D004247:DNA