Discharge of raphe magnus ON and OFF cells is predictive of the motor facilitation evoked by repeated laser stimulation.
Medullary raphe magnus (RM) on and off cells are thought to modulate spinal nociception by gating withdrawals evoked by noxious stimulation. To test whether withdrawal initiation is the target of RM modulation, we examined the relationship between on and off cell discharge and motor withdrawal evoked by noxious laser heat in halothane-anesthetized rats. The cellular responses of both cell types began during the 50 msec after onset of the tail flick, peaked within 200 msec, and outlasted the duration of the motor reaction. Thus, it is unlikely that the target of on and off cell modulation is withdrawal initiation; instead, on and off cells may modulate reactions to repeated noxious stimulation. We therefore tested whether laser heat-evoked changes in RM cell discharge were predictive of the modulatory effects of one noxious stimulus on the reaction to a subsequent noxious stimulus. Two pulses of laser heat were presented at interpulse intervals of 0.8, 2.0, or 10.0 sec. The motor withdrawal evoked by the second pulse was significantly enhanced relative to that evoked by the first pulse. The observed motor enhancement depended on supraspinal input because it was not present in spinalized rats. Comparison of the relative changes in motor and cellular activity preceding double laser heat stimulation revealed parallel changes between motor facilitation, decreases in off cell discharge, and increases in on cell discharge. This finding suggests a preparatory role for RM on and off cells in enhancing reactions to a noxious stimulus that closely follows another noxious stimulus.
Foo
H
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Department of Neurobiology, Pharmacology and Physiology, and Committee on Neurobiology, University of Chicago, Chicago, Illinois 60637, USA.
Mason
Peggy
P
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D019771:Axotomy; D004567:Electrodes, Implanted; D004576:Electromyography; D006358:Hot Temperature; D007834:Lasers; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D009433:Neural Inhibition / Q000502:physiology; D009474:Neurons / Q000145:classification / Q000502:physiology; D010147:Pain Measurement / Q000295:instrumentation / Q000379:methods*; D010812:Physical Stimulation / Q000295:instrumentation / Q000379:methods; D011903:Raphe Nuclei / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000502:physiology; D012018:Reflex / Q000502:physiology; D013116:Spinal Cord / Q000502:physiology