[Screening and cloning of genes related to varicose great saphenous vein accompanying with primary deep vein valve insufficiency].
OBJECTIVE
To screen the genes related to the occurrence and development of varicosis of the great saphenous vein in the patients with primary deep vein valve insufficiency.


METHODS
Using mRNA fluorescent differential display reverse transcriptive polymerase chain reaction (FDD-RTPCR), different genes expressed in the varicose great saphenous veins in patients with primary deep vein valve insufficiency and corresponding normal human tissues were compared. Differentially expressed cDNA fragments confirmed by Northern blot were compared and then cloned into the pGEM-Teasy vector. Positive clones were selected and sequenced. All the sequences were put into GenBank and analyzed by BLASTN software to search for their genetic origins.


RESULTS
Altogether 37 different cDNA fragments were obtained and 30 of which were confirmed by Northern blot. Analysis of the sequences by BLASTN software showed that C(610) fragment (NO. 18 cDNA clone) shared 96% homology with the mRNA sequence of the human Mckusick-Kaufman syndrome gene (MKKS gene).


CONCLUSIONS
C(610) fragment is highly homologous with the mRNA sequence of the human MKKS gene and is closely related to the development of varicosis of the great saphenous vein in patients with primary deep vein valve insufficiency.
Wang
Shenming
S
Department of Vascular Surgery, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
Yin
Henghui
H
Wang
Jinsong
J
Hu
Zuojun
Z
Huang
Xueling
X
D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D056385:Group II Chaperonins; D006801:Humans; D035002:Lower Extremity / Q000098:blood supply*; D018832:Molecular Chaperones / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012501:Saphenous Vein; D012689:Sequence Homology, Nucleic Acid; D014648:Varicose Veins / Q000209:etiology / Q000235:genetics*; D014689:Venous Insufficiency / Q000150:complications / Q000235:genetics*
C414726:MKKS protein, human; D018832:Molecular Chaperones; D012333:RNA, Messenger; D056385:Group II Chaperonins