title: Increased acetylcholine levels in skin biopsies of patients with atopic dermatitis.
abstract: Recent experimental evidence indicates that non-neuronal acetylcholine is involved in the regulation of basic cell functions. Here we investigated the cholinergic system in the skin of healthy volunteers and patients with atopic dermatitis (AD). The synthesizing enzyme, choline-acetyltransferase (ChAT), was studied by anti-ChAT immunohistochemistry and enzyme assay. Skin biopsies taken from healthy volunteers and from AD patients were separated into the 2 mm superfical (epidermis and upper dermis) and 3 mm underlying portion (deeper dermis and subcutis). ChAT enzyme activity was detected in homogenized skin and subcutaneous fat (about 13 nmol/mg protein/h). ChAT immunoreactivity was expressed in keratinocytes, hair papilla, sebaceous and eccrine sweat glands, endothelial cells and mast cells. In healthy volunteers the superficial and underlying portion of skin biopsies contained 130 +/- 30 and 550 +/- 170 pmol/g acetylcholine (n = 12), respectively. In AD patients (n = 7) acetylcholine was increased 14-fold in the superficial and 3-fold in the underlying biopsy portion. The present study demonstrates the widespread expression of ChAT protein in the vast majority of human skin cells. Tissue levels of acetylcholine are greatly (14-fold) enhanced in the superficial 2 mm skin of AD patients.
authors.affiliation: Institute of Pharmacology, University of Mainz, Obere Zahlbacher Str 67, D-55101 Mainz, Germany. wessler@uni-mainz.de
authors.forename: Ignaz
authors.initials: I
authors.lastname: Wessler
authors.forename: Torsten
authors.initials: T
authors.lastname: Reinheimer
authors.forename: Heinz
authors.initials: H
authors.lastname: Kilbinger
authors.forename: Fernando
authors.initials: F
authors.lastname: Bittinger
authors.forename: Charles James
authors.initials: CJ
authors.lastname: Kirkpatrick
authors.forename: Joachim
authors.initials: J
authors.lastname: Saloga
authors.forename: Jürgen
authors.initials: J
authors.lastname: Knop
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000328:Adult; D001706:Biopsy; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003876:Dermatitis, Atopic / Q000201:enzymology / Q000378:metabolism*; D004485:Eczema / Q000201:enzymology / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D012867:Skin / Q000201:enzymology / Q000378:metabolism*
chemical_list: D002795:Choline O-Acetyltransferase; D000109:Acetylcholine