title: Relationship between gastric disease and deletion of cag pathogenicity island genes of Helicobacter pylori in gastric juice.
abstract: The cag pathogenicity island genes of Helicobacter pylori (ie, cag1, cag5, cagT, cagE, and cagA) were detected by PCR in DNA extracted from endoscopically collected gastric juice, and the relationship between these genes and gastric disease was studied in 25 patients with early gastric cancer, 9 patients with gastric ulcer, and 15 patients with chronic active gastritis. In three patients with early gastric cancer and one patient with gastric ulcer, cag pathogenicity island genes were amplified although H. pylori was not detected by conventional methods. Compared with conventional methods, the sensitivity of detection of cag genes was 92.3% (36/39) and the specificity was 60% (6/10). Among the patients with cagA amplification, only cagE was not amplified in one case each with early cancer and chronic active gastritis. In addition, none of cag1, cag5, cagT, and cagE were amplified in spite of cagA amplification in one patient with gastric ulcer. This method is a simple procedure, has a high sensitivity, and appears to be useful for accurate assessment of infection with cagA-positive strains. Because deletion of cag PAI genes was found in the patients with all three gastric diseases that we studied, it was suggested that the pathogenicity of H. pylori might not be determined by cag PAI genes in those cases.
authors.affiliation: Department of Endoscopy and Endoscopic Surgery, Gunma University Hospital, 3-39-15, Showa-machi, Maebashi, Gunma 371-8511, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Kawamura
authors.forename: Masami
authors.initials: M
authors.lastname: Murakami
authors.forename: Osamu
authors.initials: O
authors.lastname: Araki
authors.forename: Takuro
authors.initials: T
authors.lastname: Yamada
authors.forename: Sayaka
authors.initials: S
authors.lastname: Tomizawa
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Shimoyama
authors.forename: Keiko
authors.initials: K
authors.lastname: Minashi
authors.forename: Masaki
authors.initials: M
authors.lastname: Maeda
authors.forename: Motoyasu
authors.initials: M
authors.lastname: Kusano
authors.forename: Masatomo
authors.initials: M
authors.lastname: Mori
mesh_terms: D000368:Aged; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005750:Gastric Juice / Q000382:microbiology*; D005756:Gastritis / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D013272:Stomach Diseases / Q000382:microbiology*; D013274:Stomach Neoplasms / Q000382:microbiology; D013276:Stomach Ulcer / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C080866:cagA protein, Helicobacter pylori