Allergen-induced airway disease is mouse strain dependent.
We investigated the development of airway hyperreactivity (AHR) and inflammation in the lungs of nine genetically diverse inbred strains of mice [129/SvIm, A/J, BALB/cJ, BTBR+(T)/tf/tf, CAST/Ei, C3H/HeJ, C57BL/6J, DBA/2J, and FVB/NJ] after sensitization and challenge with ovalbumin (OVA). At 24, 48, and 72 h post-OVA exposure, the severity of AHR and eosinophilic inflammation of the mouse strains ranged from relatively unresponsive to responsive. The severity of the airway eosinophilia of some strains did not clearly correlate with the development of AHR. The temporal presence of T helper type 2 cytokines in lung lavage fluid also varied markedly among the strains. The levels of IL-4 and IL-13 were generally increased in the strains with the highest airway eosinophilia at 24 and 72 h postexposure, respectively; the levels of IL-5 were significantly increased in most of the strains with airway inflammation over the 72-h time period. The differences of physiological and biological responses among the inbred mouse strains after OVA sensitization and challenge support the hypothesis that genetic factors contribute, in part, to the development of allergen-induced airway disease.
Whitehead
Gregory S
GS
Pulmonary and Critical Care Medicine, Duke University Medical Center, Research Drive, Rm. 275 MSRB, DUMC Box 2629, Durham, NC 27710-0001, USA. white141@mc.duke.edu
Walker
Julia K L
JK
Berman
Katherine G
KG
Foster
W Michael
WM
Schwartz
David A
DA
D000485:Allergens / Q000276:immunology / Q000494:pharmacology; D000818:Animals; D001249:Asthma / Q000209:etiology / Q000235:genetics* / Q000276:immunology*; D016535:Bronchial Hyperreactivity / Q000209:etiology / Q000235:genetics / Q000276:immunology; D004804:Eosinophils / Q000166:cytology; D006967:Hypersensitivity / Q000150:complications / Q000235:genetics* / Q000276:immunology*; D018793:Interleukin-13 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009504:Neutrophils / Q000166:cytology; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology; D013045:Species Specificity; D018418:Th2 Cells / Q000166:cytology / Q000378:metabolism
D000485:Allergens; D018793:Interleukin-13; D015848:Interleukin-5; D015847:Interleukin-4; D010047:Ovalbumin