title: Reconstruction of epidermis by grafting of keratinocytes cultured on polymer support--clinical study.
abstract: BACKGROUND
  Extensive wound coverage still represents a challenge for contemporary medicine. We demonstrate the results of a clinical trial of the grafting of cultured keratinocytes directly on a polymer cultivation support in the treatment of skin defects in seriously burned patients and in patients with trophic ulcers.
  
  
  METHODS
  Wound closure was evaluated clinically. The morphology and phenotypic pattern of the reconstructed epidermis, including the basal lamina, as well as the presence of Langerhans cells, were evaluated immunocytochemically using a panel of monoclonal antibodies.
  
  
  RESULTS
  All layers of the reconstructed epidermis were normally differentiated (cytokeratin immunocytochemistry). The basal lamina contained collagen type IV and laminin. The reconstructed epidermis was extensively colonized by Langerhans cells.
  
  
  CONCLUSIONS
  The results of the described technology are encouraging, especially in patients after a burn injury. The described procedure is suitable for the treatment of skin defects in clinical practice.
authors.affiliation: Prague Burn Center, 3rd Faculty of Medicine, Center of Cell Therapy and Tissue Repair, Czech Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: Dvoránková
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Holíková
authors.forename: Jirí
authors.initials: J
authors.lastname: Vacík
authors.forename: Radana
authors.initials: R
authors.lastname: Königová
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Kapounková
authors.forename: Jirí
authors.initials: J
authors.lastname: Michálek
authors.forename: Martin
authors.initials: M
authors.lastname: Prádn
authors.forename: Karel
authors.initials: K
authors.lastname: Smetana
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002056:Burns / Q000601:surgery; D017690:Cell Transplantation; D002478:Cells, Cultured; D002648:Child; D004817:Epidermis / Q000601:surgery*; D005260:Female; D006801:Humans; D007223:Infant; D015603:Keratinocytes / Q000637:transplantation*; D008297:Male; D008875:Middle Aged; D011108:Polymers; D012883:Skin Ulcer / Q000601:surgery; D023822:Tissue Engineering; D020858:Tissue and Organ Harvesting / Q000534:rehabilitation; D014945:Wound Healing
chemical_list: D011108:Polymers