title: [Techniques for preparing postmortem human eyes to perform anterior segment intraocular surgery].
abstract: We describe different methods to prepare postmortem human or animal eyes used at the Center for Research in Ocular Therapeutics and Biodevices at the Storm Eye Institute, Medical University of South Carolina, Charleston, SC, USA. These techniques have been utilized for performing different surgical procedures (phacoemulsification, extracapsular cataract extraction, etc.), and for training of surgeons in-transition. Performing these techniques in the wet-laboratory contributed to improve surgical skills to perform the critical steps of the phacoemulsification surgery. Pathological evaluation of pseudophakic postmortem human eyes using the Miyake-Apple posterior view and histology was helpful to analyze postoperative complications of cataract surgery (anterior capsule opacification and posterior capsule opacification) secondary to postoperative proliferation of lens epithelial cells into the capsular bag. Modifications in the surgical techniques and/or lens design may be helpful to reduce these postoperative complications. Implantation of various aphakic and phakic intraocular lenses in postmortem human eyes as well as animal eyes was helpful to analyze the sizing and fitting of new lens designs within the eye.
authors.affiliation: Center for Research on Ocular Therapeutics and Biodevices, Storm Eye Institute, Medical University of South Carolina, USA. Luis_Vargas@med.uni-heidelberg.de
authors.forename: L G
authors.initials: LG
authors.lastname: Vargas
authors.forename: L
authors.initials: L
authors.lastname: Werner
authors.forename: S K
authors.initials: SK
authors.lastname: Pandey
authors.forename: L P
authors.initials: LP
authors.lastname: Werner
authors.forename: J M
authors.initials: JM
authors.lastname: Schmidbauer
authors.forename: V
authors.initials: V
authors.lastname: Zuleta
authors.forename: M
authors.initials: M
authors.lastname: Escobar-Gómez
authors.forename: D J
authors.initials: DJ
authors.lastname: Apple
mesh_terms: D000818:Animals; D000869:Anterior Eye Segment / Q000601:surgery*; D002102:Cadaver; D002387:Cataract Extraction / Q000009:adverse effects / Q000193:education* / Q000379:methods*; D004396:Coloring Agents; D006801:Humans; D007910:Lenses, Intraocular; D011183:Postoperative Complications / Q000175:diagnosis
chemical_list: D004396:Coloring Agents