title: In vivo studies of liver-type fatty acid binding protein (L-FABP) gene expression in liver of transgenic zebrafish (Danio rerio).
abstract: Mammalian liver fatty acid binding protein (L-FABP) is a small cytosolic protein in various tissues including liver, small intestine and kidney and is thought to play a crucial role in intracellular fatty acid trafficking and metabolism. To better understand its tissue-specific regulation during zebrafish hepatogenesis, we isolated 5'-flanking sequences of the zebrafish L-FABP gene and used a green fluorescent protein (GFP) transgenic strategy to generate liver-specific transgenic zebrafish. The 2.8-kb 5'-flanking sequence of zebrafish L-FABP gene was sufficient to direct GFP expression in liver primordia, first observed in 2 dpf embryos and then continuously to the adult stage. This pattern of transgenic expression is consistent with the expression pattern of the endogenous gene. F2 inheritance rates of 42-51% in all the seven transgenic lines were consistent with the ratio of Mendelian segregation. Further, hhex and zXbp-1 morphants displayed a visible liver defect, which suggests that it is possible to establish an in vivo system for screening genes required for liver development.
authors.affiliation: Laboratory of Marine Molecular Biology and Biotechnology, Institute of Zoology, Academia Sinica, Nankang, Taipei 115, Taiwan.
authors.forename: Guor Mour
authors.initials: GM
authors.lastname: Her
authors.forename: Chia-Chang
authors.initials: CC
authors.lastname: Chiang
authors.forename: Wen-Ya
authors.initials: WY
authors.lastname: Chen
authors.forename: Jen-Leih
authors.initials: JL
authors.lastname: Wu
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D049452:Green Fluorescent Proteins; D008099:Liver / Q000378:metabolism*; D008164:Luminescent Proteins / Q000378:metabolism; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D016133:Polymerase Chain Reaction; D015027:Zebrafish; D029961:Zebrafish Proteins
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; C493320:Fabp1 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D008164:Luminescent Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D029961:Zebrafish Proteins; C490175:fabp7a protein, zebrafish; D049452:Green Fluorescent Proteins