Developmental regulation of beta-1,3-galactosyltransferase-1 gene expression in mouse brain.
beta-1,3-galactosyltransferase-1 (beta3GalT-1) is the key enzyme to form the type 1 chain structure. Northern blot analysis indicated that beta3GalT-1 was expressed predominantly in the brain. In the present study, it was revealed that the gene expression of beta3GalT-1 in mouse brain was developmentally decreased. High expression levels of beta3GalT-1 were found in cerebral cortex and hippocampus in both newborn and adult mice, while in cerebellum, the expression levels decreased markedly during development. In situ hybridization revealed that the absence of expression in cerebellar granual cell layers contributed to the main loss of beta3GalT-1 expression in adult mouse cerebellum. Moreover, the decreased levels of beta3GalT-1 could affect the synthesis of type 1 chain oligosaccharides, as revealed by immunohistochemistry analysis.
Zhu
Dan
D
Box 103, Gene Research Center, Shanghai Medical College of Fudan University (Former Shanghai Medical University), Shanghai 200032, PR China.
Shen
Aiguo
A
Wang
Ying
Y
Gu
Xiaosong
X
Gu
Jianxin
J
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000196:embryology / Q000201:enzymology*; D017931:DNA Primers; D005260:Female; D005700:Galactosyltransferases / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics
D017931:DNA Primers; D012333:RNA, Messenger; D005700:Galactosyltransferases