Bacteriocin-like substance (BLS) production in Aeromonas hydrophila water isolates.
30 Aeromonas hydrophila water isolates were tested for bacteriocin-like substance (BLS) production using a target panel of closely related microorganisms and other Gram-positive and Gram-negative bacteria, including food-borne pathogens. A. hydrophila showed antibacterial activity against one or more indicator microorganisms, but the activity emerged only with non-phylogenetically related genera or species. In particular all A. hydrophila showed antibacterial activity against one or more of the tested Staphylococcus strains, five against Listeria spp. (Listeria seeligeri, Listeria welshimeri and Listeria ivanovii), and eight presented a weak antagonistic activity towards Streptococcus agalactiae and Lactobacillus spp. Inhibitory activity was not observed against the other Gram-positive (Listeria monocytogenes, Listeria innocua and Enterococcus spp.) and Gram-negative tested strains, including Aeromonas sobria, Aeromonas caviae and the same A. hydrophila, when used as indicator. Anti-staphylococcal activity was observed with a gradual increase of the inhibition zone during incubation and seemed to be influenced by A. hydrophila hemolytic expression. Extrachromosomal analysis showed the presence, in 70% of the strains, of one to five plasmids with molecular masses ranging from 2.1 to 41.5 MDa, but it was not possible to relate this result with BLS production.
Messi
Patrizia
P
Department of Biomedical Sciences, University of Modena and Reggio E., Via Campi 287, 41100 Modena, Italy.
Guerrieri
Elisa
E
Bondi
Moreno
M
D016980:Aeromonas hydrophila / Q000302:isolation & purification / Q000378:metabolism*; D001430:Bacteriocins / Q000096:biosynthesis* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D005516:Food Microbiology; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008087:Listeria / Q000187:drug effects; D010957:Plasmids; D011549:Pseudomonas / Q000187:drug effects; D013210:Staphylococcus / Q000187:drug effects; D014871:Water Microbiology
D001430:Bacteriocins