title: Slit and glypican-1 mRNAs are coexpressed in the reactive astrocytes of the injured adult brain.
abstract: The slit family serves as a repellent for growing axons toward correct targets during neural development. A recent report describes slit mRNAs expressed in various brain regions in adult rats. However, their functions in the adult nervous system remain unknown. In the present study, we investigated whether slit mRNAs were expressed in the cryo-injured brain, using in situ hybridization. All slit family members were expressed at the lesion. Slit2 mRNA was the most intensely expressed in the cells surrounding the necrotic tissue. A double-labeling study showed that slit2 mRNA was expressed in the glial fibrillary acidic protein (GFAP)-positive reactive astrocytes. In addition, glypican-1, a heparan sulfate proteoglycan that serves as a high-affinity receptor for Slit protein, was coexpressed with slit2 mRNA in the reactive astrocytes. These findings suggested that slit2 might prevent regenerating axons from entering into the lesion in concert with glypican-1.
authors.affiliation: Department of Orthopedic Surgery, Fukushima Medical University School of Medicine, Fukushima, Japan. shagino@fmu.ac.jp
authors.forename: Seita
authors.initials: S
authors.lastname: Hagino
authors.forename: Ken
authors.initials: K
authors.lastname: Iseki
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Mori
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Hikake
authors.forename: Sachihiko
authors.initials: S
authors.lastname: Yokoya
authors.forename: Mayumi
authors.initials: M
authors.lastname: Takeuchi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Hasimoto
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kikuchi
authors.forename: Akio
authors.initials: A
authors.lastname: Wanaka
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D015415:Biomarkers; D001930:Brain Injuries / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D002135:Calcium-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005911:Gliosis / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D020439:Growth Cones / Q000378:metabolism*; D019812:Heparan Sulfate Proteoglycans / Q000235:genetics*; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008840:Microfilament Proteins; D017628:Microglia / Q000166:cytology / Q000378:metabolism; D009416:Nerve Regeneration / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060225:Tetraspanin 28; D000098552:Slit Homolog 2 Protein
chemical_list: C491049:Aif1 protein, mouse; D015703:Antigens, CD; D015415:Biomarkers; D002135:Calcium-Binding Proteins; C554799:Cd81 protein, mouse; C554797:Cd81 protein, rat; D005904:Glial Fibrillary Acidic Protein; D019812:Heparan Sulfate Proteoglycans; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C120010:Slit1 protein, mouse; C492288:Slit1 protein, rat; C432251:Slit3 protein, mouse; D060225:Tetraspanin 28; D000098552:Slit Homolog 2 Protein