title: Determination of organic acids in tobacco by capillary isotachophoresis.
abstract: Nine organic acids and phosphate have been separated and quantified in tobacco by capillary isotachophoresis. Two operating systems for the separation were found: hydrochloric acid (10 mmol l(-1)) adjusted with beta-alanine to pH 2.9 plus 0.1% poly(vinylpyrrolidone) was used as the leading electrolyte and 10 mmol l(-1) nicotinic acid as the terminating electrolyte and hydrochloric acid (10 mmol l(-1)) including 5.5 mmol l(-1) 1,3-bis[tris(hydroxymethyl)methylamino]propane plus 0.1% poly(vinylpyrrolidone) was used as the leading electrolyte and 5 mmol l(-1) 2-morpholinoethanesulfonic acid as the terminating electrolyte. Linearity was observed from 0.008 to 0.100 mmol l(-1) with a coefficient of determination (r2) of 0.999. The separation of anions was achieved in less than 16 min. The minimal sample pretreatment and relatively low running cost make isotachophoresis a good alternative to existing methods.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemical and Food Technology, Radlinského 9, 812 37 Bratislava, Slovak Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Sádecká
authors.forename: Jozef
authors.initials: J
authors.lastname: Polonský
mesh_terms: D000143:Acids / Q000032:analysis*; D004586:Electrophoresis / Q000379:methods*; D009930:Organic Chemicals / Q000032:analysis*; D015203:Reproducibility of Results; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D000143:Acids; D009930:Organic Chemicals