title: Human acid sphingomyelinase.
abstract: Human acid sphingomyelinase (haSMase, EC 3.1.4.12) catalyzes the lysosomal degradation of sphingomyelin to ceramide and phosphorylcholine. An inherited haSMase deficiency leads to Niemann-Pick disease, a severe sphingolipid storage disorder. The enzyme was purified and cloned over 10 years ago. Since then, only a few structural properties of haSMase have been elucidated. For understanding of its complex functions including its role in certain signaling and apoptosis events, complete structural information about the enzyme is necessary. Here, the identification of the disulfide bond pattern of haSMase is reported for the first time. Functional recombinant enzyme expressed in SF21 cells using the baculovirus expression system was purified and digested by trypsin. MALDI-MS analysis of the resulting peptides revealed the four disulfide bonds Cys120-Cys131, Cys385-Cys431, Cys584-Cys588 and Cys594-Cys607. Two additional disulfide bonds (Cys221-Cys226 and Cys227-Cys250) which were not directly accessible by tryptic cleavage, were identified by a combination of a method of partial reduction and MALDI-PSD analysis. In the sphingolipid activator protein (SAP)-homologous N-terminal domain of haSMase, one disulfide bond was assigned as Cys120-Cys131. The existence of two additional disulfide bridges in this region was proved, as was expected for the known disulfide bond pattern of SAP-type domains. These results support the hypothesis that haSMase possesses an intramolecular SAP-type activator domain as predicted by sequence comparison [Ponting, C.P. (1994) Protein Sci., 3, 359-361]. An additional analysis of haSMase isolated from human placenta shows that the recombinant and the native human protein possess an identical disulfide structure.
authors.affiliation: Kekulé-Institut für Organische Chemie und Biochemie, Universität Bonn, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Lansmann
authors.forename: Christina G
authors.initials: CG
authors.lastname: Schuette
authors.forename: Oliver
authors.initials: O
authors.lastname: Bartelsen
authors.forename: Joerg
authors.initials: J
authors.lastname: Hoernschemeyer
authors.forename: Thomas
authors.initials: T
authors.lastname: Linke
authors.forename: Judith
authors.initials: J
authors.lastname: Weisgerber
authors.forename: Konrad
authors.initials: K
authors.lastname: Sandhoff
mesh_terms: D000595:Amino Acid Sequence; D002518:Ceramides / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry*; D006031:Glycosylation; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009542:Niemann-Pick Diseases / Q000201:enzymology; D016366:Open Reading Frames; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010720:Phosphines / Q000737:chemistry; D010767:Phosphorylcholine / Q000378:metabolism; D010920:Placenta / Q000201:enzymology; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013108:Sphingomyelin Phosphodiesterase / Q000737:chemistry* / Q000235:genetics; D013109:Sphingomyelins / Q000378:metabolism
chemical_list: D002518:Ceramides; D004220:Disulfides; D007202:Indicators and Reagents; D010455:Peptides; D010720:Phosphines; D010767:Phosphorylcholine; D011994:Recombinant Proteins; D013108:Sphingomyelin Phosphodiesterase; D013109:Sphingomyelins; C080938:tris(2-carboxyethyl)phosphine