Comparison of the susceptibility testing of clinical isolates of Mycobacterium tuberculosis by the XTT colorimetric method and the NCCLS standards method.
The susceptibility or resistance of clinical isolates of Mycobacterium tuberculosis were determined by a method incorporating the 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and compared with results obtained by the National Committee for Clinical Laboratory Standards approved standard method (M24-T2). One hundred percent of all isolates demonstrated agreement between the susceptibility and resistance to isoniazid, rifampicin, and ethambutol obtained by the two methods, suggesting that the XTT-based method could provide a useful means for the rapid determination of antimycobacterial susceptibility of clinical isolates of M. tuberculosis.
De Logu
Alessandro
A
Dipartimento di Scienze Chirurgiche e Trapianti d'Organo, Sezione di Microbiologia e Virologia, Università di Cagliari, Viale Frà Ignazio 38, 09123 Cagliari, Italy. adelogu@unica.it
Pellerano
Maria Luisa
ML
Sanna
Adriana
A
Pusceddu
Maria Cristina
MC
Uda
Patrizia
P
Saddi
Barbara
B
D000995:Antitubercular Agents / Q000494:pharmacology; D015169:Colony Count, Microbial; D003124:Colorimetry / Q000379:methods; D024881:Drug Resistance, Bacterial; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D013778:Tetrazolium Salts; D018088:Tuberculosis, Multidrug-Resistant / Q000188:drug therapy / Q000382:microbiology
D000995:Antitubercular Agents; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide