Reconstruction of epidermis by grafting of keratinocytes cultured on polymer support--clinical study.
BACKGROUND
Extensive wound coverage still represents a challenge for contemporary medicine. We demonstrate the results of a clinical trial of the grafting of cultured keratinocytes directly on a polymer cultivation support in the treatment of skin defects in seriously burned patients and in patients with trophic ulcers.


METHODS
Wound closure was evaluated clinically. The morphology and phenotypic pattern of the reconstructed epidermis, including the basal lamina, as well as the presence of Langerhans cells, were evaluated immunocytochemically using a panel of monoclonal antibodies.


RESULTS
All layers of the reconstructed epidermis were normally differentiated (cytokeratin immunocytochemistry). The basal lamina contained collagen type IV and laminin. The reconstructed epidermis was extensively colonized by Langerhans cells.


CONCLUSIONS
The results of the described technology are encouraging, especially in patients after a burn injury. The described procedure is suitable for the treatment of skin defects in clinical practice.
Dvoránková
Barbora
B
Prague Burn Center, 3rd Faculty of Medicine, Center of Cell Therapy and Tissue Repair, Czech Republic.
Holíková
Zuzana
Z
Vacík
Jirí
J
Königová
Radana
R
Kapounková
Zuzana
Z
Michálek
Jirí
J
Prádn
Martin
M
Smetana
Karel
K
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002056:Burns / Q000601:surgery; D017690:Cell Transplantation; D002478:Cells, Cultured; D002648:Child; D004817:Epidermis / Q000601:surgery*; D005260:Female; D006801:Humans; D007223:Infant; D015603:Keratinocytes / Q000637:transplantation*; D008297:Male; D008875:Middle Aged; D011108:Polymers; D012883:Skin Ulcer / Q000601:surgery; D023822:Tissue Engineering; D020858:Tissue and Organ Harvesting / Q000534:rehabilitation; D014945:Wound Healing
D011108:Polymers