title: A preliminary linkage map of the hard tick, Ixodes scapularis.
abstract: A linkage map of the Ixodes scapularis genome was constructed, based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence-Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 F1 intercross progeny from a single, field-collected P1 female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of approximately 109 bp, the relationship of physical to genetic distance was found to be approximately 300 kb/cM in the I. scapularis genome.
authors.affiliation: Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA. aff1@cdc.gov
authors.forename: A J
authors.initials: AJ
authors.lastname: Ullmann
authors.forename: J
authors.initials: J
authors.lastname: Piesman
authors.forename: M C
authors.initials: MC
authors.lastname: Dolan
authors.forename: W C Black
authors.initials: WC
authors.lastname: Iv
mesh_terms: D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D003433:Crosses, Genetic; D005260:Female; D015723:Gene Library; D017344:Genes, Insect / Q000235:genetics*; D005819:Genetic Markers; D016678:Genome; D018884:Ixodes / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats; D018807:Polymorphism, Single-Stranded Conformational; D019105:Random Amplified Polymorphic DNA Technique; D016324:Sequence Tagged Sites
chemical_list: D005819:Genetic Markers