title: IL-7 receptor is present on human microvascular endothelial cells.
abstract: Interleukin-7 (IL-7) is a pleiotropic, non-redundant cytokine crucial for development of B and T lymphocytes. The cellular response to IL-7 is triggered by binding of the cytokine to its receptor, IL-7R. Until now the expression of the receptor was evidenced only in lymphoid and myeloid cell lineages. The receptor consists of two chains: IL-7 specific alpha chain (CD127) and the common gamma(c) chain (CD132) which is a component of several other cytokine receptors: IL-2, IL-4, IL-9 and IL-15. The former observation that exogenous IL-7 is biologically active towards murine endothelial cell lines from secondary lymphoid organs was the starting point of our studies. This observation has prompted us to search for the presence of IL-7 receptor in human microvascular endothelial cells. We used in our studies a set of human endothelial cell lines established from various organs. Our results demonstrate the presence of IL-7R in human microvascular endothelial cells, mainly in the mesenteric but also in the peripheral and to a lesser extent, in the mucosa-associated lymph node endothelial cells. On the basis of the RT-PCR reaction, molecular weight estimated in Western blot and IL-7 binding activity the identified endothelial IL-7 receptor was identical to the lymphocyte-type IL7-R.
authors.affiliation: Laboratory of Cellular Interactions, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Centre of Excellence 'IMMUNE', R. Weigla 12, 53-114, Wroclaw, Poland. danuta@immuno.iitd.pan.wroc.pl
authors.forename: Danuta
authors.initials: D
authors.lastname: Duś
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Krawczenko
authors.forename: Piotr
authors.initials: P
authors.lastname: Załecki
authors.forename: Maria
authors.initials: M
authors.lastname: Paprocka
authors.forename: Antoni
authors.initials: A
authors.lastname: Wiedłocha
authors.forename: Caroline
authors.initials: C
authors.lastname: Goupille
authors.forename: Claudine
authors.initials: C
authors.lastname: Kieda
mesh_terms: D002460:Cell Line; D004730:Endothelium, Vascular / Q000737:chemistry*; D006801:Humans; D020014:K562 Cells; D008163:Luminescent Measurements; D008833:Microcirculation / Q000166:cytology; D012333:RNA, Messenger / Q000378:metabolism; D020395:Receptors, Interleukin-7 / Q000032:analysis* / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D020395:Receptors, Interleukin-7