Cyclic guanosine monophosphate phosphodiesterase activity in human gingival carcinoma.
BACKGROUND
Cyclic guanosine monophosphate (cGMP) is an essential second messenger metabolized by phosphodiesterases (PDEs).


OBJECTIVE
We looked for a possible correlation of PDE activities in human oral squamous cell carcinoma (OSCC) with and without lymph node metastases.


METHODS
The analysis of phosphodiesterase activity and the cGMP assay were done by reverse-phase HPLC on samples of fresh or frozen gingival tissues. Analysis of cGMP was confirmed with the enzyme-linked immunoabsorption assay.


CONCLUSIONS
cGMP PDE activity was 34.92 +/- 7.17 SD, 12.89 +/- 4.43 SD, and 35.88 +/- 8.76 SD (nmols/mg of protein), respectively, in controls, samples without lymph node involvement (N-), and specimens with lymph node metastases (N+). cGMP values were 1.97 +/- 0.63 SD, 3.30 +/- 1.47 SD, and 3.49 +/- 1.47 SD (nmols/mg of protein). Our data support the hypothesis of a role for cGMP and PDE in the progression of OSCC.
Spoto
Giuseppe
G
Department of Applied Sciences of Oral and Dental Diseases, G. D'Annunzio University, Via dei Vestini 31, 66013 Chieti, Italy. spoto@unich.it
Fioroni
Massimiliano
M
Rubini
Corrado
C
Contento
Alessandro
A
Di Nicola
Maurizio
M
Forcella
Sabrina
S
Piattelli
Adriano
A
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000556:secondary; D006152:Cyclic GMP / Q000378:metabolism / Q000502:physiology; D018450:Disease Progression; D005881:Gingiva / Q000201:enzymology; D005887:Gingival Neoplasms / Q000201:enzymology*; D006801:Humans; D008198:Lymph Nodes / Q000201:enzymology; D008207:Lymphatic Metastasis; D015290:Second Messenger Systems / Q000502:physiology; D013223:Statistics as Topic
D015106:3',5'-Cyclic-GMP Phosphodiesterases; D006152:Cyclic GMP