CD8alpha+ mouse spleen dendritic cells do not originate from the CD8alpha- dendritic cell subset.
Although previous studies had indicated that the CD8alpha- and CD8alpha+ subtypes of murine dendritic cells (DCs) differ in immediate origin, a recent study found that intravenous transfer of CD8alpha- DCs led to CD8alpha+ DCs in the spleen several days later, suggesting a direct precursor-product relationship. We have repeated these experiments with a balance sheet approach. We find that though a few CD8alpha+ DCs can be generated in such experiments, this is a rare event and could be the result of a contaminant precursor. Most of the immediate precursors of CD8alpha+ DCs are cells that lack the phenotype of a recognizable DC. CD8alpha- DCs and CD8alpha+ DCs are not precursor-product related, though these sublineages may be connected further upstream.
Naik
Shalin
S
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
Vremec
David
D
Wu
Li
L
O'Keeffe
Meredith
M
Shortman
Ken
K
D000818:Animals; D016827:CD8 Antigens / Q000032:analysis*; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation; D002678:Chimera / Q000276:immunology; D003713:Dendritic Cells / Q000145:classification / Q000166:cytology* / Q000637:transplantation; D005434:Flow Cytometry; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology*
D016827:CD8 Antigens; C431698:CD8alpha antigen