title: In vivo transglutaminase type 1 expression in normal lung, preinvasive bronchial lesions, and lung cancer.
abstract: Transglutaminase type 1 (TGase 1) is a member of a class of enzymes that catalyze the cross-linking of proteins, a characteristic feature of epidermal differentiation and squamous metaplasia. The role of TGase 1 has been extensively studied in epidermis but not in the lung. Using a polyclonal anti-TGase 1 antibody prepared in our laboratory (TGase-lac), TGase 1 expression in normal bronchial epithelium, bronchial preinvasive lesions, and lung cancer was characterized. The specificity of the antibody was confirmed by the presence in squamous differentiated bronchial cells of specific 106-kD and 92-kD bands by Western blotting. In addition, immunohistochemistry displayed a recognized pattern of labeling in both normal and tumor cells beneath the cytoplasmic membrane and within the cytosol. TGase 1 was shown to be expressed by cells from bronchial epithelium and bronchial preinvasive lesions, strongly expressed in most non-small-cell lung cancer tumor cells and in apoptotic bodies, but weakly expressed in small-cell lung cancer. The distribution of TGase 1 mRNA correlated with the immunohistochemical profile. These observations suggest that TGase 1 expression is a normal feature of bronchial epithelium and is linked to the process of squamous differentiation occurring in preinvasive lesions. Its role in lung cancer remains to be clarified.
authors.affiliation: EA 3443 and Laboratoire d'Hématologie, Faculté de Médecine, Vandoeuvre-Les-Nancy, Nancy, France.
authors.forename: Nadine
authors.initials: N
authors.lastname: Martinet
authors.forename: Lionel
authors.initials: L
authors.lastname: Bonnard
authors.forename: Véronique
authors.initials: V
authors.lastname: Regnault
authors.forename: Evelyne
authors.initials: E
authors.lastname: Picard
authors.forename: Louise
authors.initials: L
authors.lastname: Burke
authors.forename: Joëlle
authors.initials: J
authors.lastname: Siat
authors.forename: Gilles
authors.initials: G
authors.lastname: Grosdidier
authors.forename: Yves
authors.initials: Y
authors.lastname: Martinet
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Vignaud
mesh_terms: D000918:Antibody Specificity; D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology / Q000473:pathology / Q000601:surgery; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008168:Lung / Q000201:enzymology*; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology / Q000601:surgery; D009361:Neoplasm Invasiveness; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000201:enzymology* / Q000473:pathology; D012016:Reference Values; D020545:Respiratory Mucosa / Q000201:enzymology; D012867:Skin / Q000201:enzymology; D011503:Transglutaminases / Q000235:genetics* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D011503:Transglutaminases; C105804:transglutaminase 1