title: Protective efficacy of anti-Helicobacter pylori immunity following systemic immunization of neonatal mice.
abstract: Helicobacter pylori infection of the gastric mucosa is a significant cause of morbidity and mortality because of its etiologic role in symptomatic gastritis, peptic ulcer disease, and gastric adenocarcinoma. Infection occurs in young children; therefore, a prophylactic vaccine would have to be administered within the first year of life, a period thought to be immunologically privileged. We investigated vaccine formulations administered by different routes to confer protective anti-H. pylori immunity in neonatal mice. Neonatal mice immunized with a single dose of vaccine in complete Freund's adjuvant (CFA) generated antigen-specific gamma interferon-, interleukin-2 (IL-2)-, IL-4-, and IL-5-secreting T cells in numbers similar to those in immunized adult mice, while vaccine administered to neonates in incomplete Freund's adjuvant (IFA) induced such cells in reduced numbers compared to those in adult mice. Both IFA and CFA, however, provided partial protection from a challenge with infectious H. pylori when the vaccine was administered subcutaneously. Neonatal immunized mice also had reduced bacterial loads when immunized intraperitoneally with CFA. In all cases, protection was equivalent to that achieved when adult counterparts were immunized. These studies suggest that an efficacious vaccine might be successfully administered to very young children to prevent perinatal infection of H. pylori.
authors.affiliation: Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
authors.forename: Julia C
authors.initials: JC
authors.lastname: Eisenberg
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Czinn
authors.forename: Christine A
authors.initials: CA
authors.lastname: Garhart
authors.forename: Raymond W
authors.initials: RW
authors.lastname: Redline
authors.forename: Wolf C
authors.initials: WC
authors.lastname: Bartholomae
authors.forename: Judith M
authors.initials: JM
authors.lastname: Gottwein
authors.forename: John G
authors.initials: JG
authors.lastname: Nedrud
authors.forename: Steven E
authors.initials: SE
authors.lastname: Emancipator
authors.forename: Bernhard B
authors.initials: BB
authors.lastname: Boehm
authors.forename: Paul V
authors.initials: PV
authors.lastname: Lehmann
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Blanchard
mesh_terms: D000536:Aluminum Hydroxide / Q000276:immunology; D000818:Animals; D000831:Animals, Newborn; D000942:Antigens, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D005620:Freund's Adjuvant / Q000276:immunology*; D016481:Helicobacter Infections / Q000276:immunology / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000276:immunology*; D007114:Immunization; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D016207:Cytokines; D000536:Aluminum Hydroxide; D005620:Freund's Adjuvant