title: Polyprotein processing protease of African swine fever virus: purification and biochemical characterization.
abstract: The purified recombinant African swine fever virus polyprotein processing protease cleaves the two GG-X sites in polyprotein pp62 with the same efficiency. Cleavage at the site that is first recognized in vivo is not a requisite for cleavage at the second site, suggesting the existence of mechanisms that control the ordered processing of the polyprotein during infection.
authors.affiliation: Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid), Universidad Autónoma de Madrid, Cantoblanco, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Rubio
authors.forename: Alí
authors.initials: A
authors.lastname: Alejo
authors.forename: Irene
authors.initials: I
authors.lastname: Rodríguez
authors.forename: María L
authors.initials: ML
authors.lastname: Salas
mesh_terms: D000358:African Swine Fever Virus / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D010450:Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005814:Genes, Viral; D066298:In Vitro Techniques; D009154:Mutation; D020815:Polyproteins / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D025842:SUMO-1 Protein / Q000378:metabolism; D013379:Substrate Specificity; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D020815:Polyproteins; D011994:Recombinant Proteins; D025842:SUMO-1 Protein; D014764:Viral Proteins; D010450:Endopeptidases