High molecular weight factor in FCS inhibits Helicobacter pylori VacA-binding to its receptor, RPTPbeta, on AZ-521.
VacA, a secretory product of Helicobacter pylori, binds to its cell surface receptor, receptor tyrosine phosphatase (RPTP) beta, leading to cytoplasmic vacuolization of gastric epithelial AZ-521 cells. VacA binding to the cell surface and VacA-dependent vacuolization were inhibited by cell culture media containing fetal calf serum (FCS). The high molecular weight fraction of FCS isolated by Superose 12 gel filtration chromatography inhibited VacA binding, whereas only weak effects were observed with other fractions. These data show that the high molecular weight fraction of FCS inhibits VacA action though its ability to block toxin binding to its receptor, RPTPbeta, on AZ-521 cells.
Kimura
Takahiro
T
Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Nagasaki 852-8523, Japan.
Wada
Akihiro
A
Nakayama
Masaaki
M
Ogushi
Ken-ichi
K
Nishi
Yoshito
Y
De Guzman
Blanquita B
BB
Moss
Joel
J
Hirayama
Toshiya
T
D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000378:metabolism; D003603:Cytotoxins / Q000276:immunology; D005312:Fetal Blood / Q000276:immunology*; D005434:Flow Cytometry; D016481:Helicobacter Infections / Q000276:immunology*; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5; D013272:Stomach Diseases / Q000276:immunology*; D014407:Tumor Cells, Cultured; D014617:Vacuoles / Q000276:immunology
D001426:Bacterial Proteins; D003603:Cytotoxins; D009419:Nerve Tissue Proteins; C074689:VacA protein, Helicobacter pylori; C516895:PTPRZ1 protein, human; D017027:Protein Tyrosine Phosphatases; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5