title: Chromosomal alterations in osteosarcoma cell lines revealed by comparative genomic hybridization and multicolor karyotyping.
abstract: We characterized the chromosomal alterations in eight osteosarcoma cell lines (OST, HOS, U-2 OS, ZK-58, MG-63, SJSA-1, Saos-2, and MNNG) by comparative genomic hybridization (CGH); gains and losses of DNA sequences were defined as chromosomal regions with a fluorescence ratio, wherein all of the 95% confidence interval was above 1.25 and below 0.75, respectively. In four of 8 cell lines, multicolor karyotyping (MK) was added. CGH revealed the average number of aberrations per cell line was 20.8 (range: 10-31); the average numbers of gains and losses were 11.1 and 9.6, respectively. The frequent gains were identified on 1p21 approximately q24, 1q25-q31, 7p21, 7q31, 8q23 approximately q24, and 14q21; frequent losses were at 18q21 approximately q22, 18q12, 19p, and 3p12 approximately p14. High-level gains were observed on 8q23 approximately q24, 5p, and 1p21 approximately p22. MK revealed the most common translocations in the four cell lines were t(8;9), t(1;3), t(3;5), t(1;13), t(2;6), t(3; 17), t(1;15), t(10;20), and t(6;20). Chromosomes 1, 3, 8, 9, and 20 were most frequently involved in translocation events. The concordance rate of aberrations in CGH and translocations in MK was 76%. MK was useful to identify the chromosomal alterations and as a supplement to the CGH results in three of four chromosomes.
authors.affiliation: Department of Orthopaedics, Westfälische Wilhelms-University, Münster, Germany. tozaki@md.okayama-u.ac.jp
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Ozaki
authors.forename: Thomas
authors.initials: T
authors.lastname: Neumann
authors.forename: Daniel
authors.initials: D
authors.lastname: Wai
authors.forename: Karl-Ludwig
authors.initials: KL
authors.lastname: Schäfer
authors.forename: Franz
authors.initials: F
authors.lastname: van Valen
authors.forename: Norbert
authors.initials: N
authors.lastname: Lindner
authors.forename: Christina
authors.initials: C
authors.lastname: Scheel
authors.forename: Wermer
authors.initials: W
authors.lastname: Böcker
authors.forename: Winfried
authors.initials: W
authors.lastname: Winkelmann
authors.forename: Barbara
authors.initials: B
authors.lastname: Dockhorn-Dworniczak
authors.forename: Jürgen
authors.initials: J
authors.lastname: Horst
authors.forename: Christopher
authors.initials: C
authors.lastname: Poremba
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology; D002869:Chromosome Aberrations; D020223:Chromosome Painting; D002877:Chromosomes, Human / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007621:Karyotyping; D008297:Male; D009693:Nucleic Acid Hybridization; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured / Q000648:ultrastructure