Assembly of single-shelled cores and double-shelled virus-like particles after baculovirus expression of major structural proteins P3, P7 and P8 of Rice dwarf virus.
Expression of the core capsid protein P3 of Rice dwarf virus in a baculovirus system resulted in the formation of single-shelled core-like particles in insect cells in the absence of any other capsid proteins. Double-shelled virus-like particles were also observed upon mixing or co-expression of P3 and the major outer capsid protein P8, suggesting that P3 and P8 have the ability to form double-shelled particles both in vivo and in vitro. Core protein P7 expressed in a similar manner was incorporated into the virus-like particles.
Hagiwara
Kyoji
K
Laboratory of Virology, National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan.
Higashi
Takahiko
T
Laboratory of Virology, National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan.
Namba
Kazunori
K
Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan.
Uehara-Ichiki
Tamaki
T
Laboratory of Virology, National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan.
Omura
Toshihiro
T
Laboratory of Virology, National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan.
D000818:Animals; D016367:Baculoviridae / Q000378:metabolism* / Q000648:ultrastructure; D002460:Cell Line; D015870:Gene Expression; D005822:Genetic Vectors; D019590:Nucleocapsid Proteins / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D012087:Reoviridae / Q000235:genetics*; D015678:Viral Structural Proteins / Q000096:biosynthesis / Q000235:genetics*
D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins; D015678:Viral Structural Proteins