[Features of regulating delta sleep-inducing peptide by free radical processes in tissue and erythrocyte membranes in intact animals and under stress].
In activity the comparative analysis of metabolic effects delta--sleep inducing peptide (DSIP) in tissues and erythrocytes of intact rats and under cold stress is conducted. The regulation effect of DSIP in attitude of free radical processes will be realised through modulation the prooxidant--antioxidant balance: both for intact animal, and at stress. Exogenous DSIP increases the antioxidant system activity in tissues of brain, liver and blood in standard conditions and under cold stress. The anti-stress effect of DSIP is directed as on increase of power endogenic enzymatic antioxidant system activity, specially glutathione peroxidase activity, and not enzymatic of antioxidant protection. The DSIP renders different influence on activity of prooxidant enzymes: for intact animal boosts the myeloperoxidase activity in blood neutrophils, not rendering essential influencing on the xanthine oxidase activity in tissues of brain, liver and activates the myeloperoxidase activity, depresses the xanthine oxidase activity for rats at stress. The membranotropic effect of DSIP in the norm and under stress is connected to increase of stability of protein--lipid interplays. The membranostabilizing effect of DSIP in conditions of stress is characterized decrease of polarity of lipid phase and negative surface charge of erythrocyte membranes, modified in course of lipid peroxidation.
Shustanova
T A
TA
Rostov State University, Rostov-on-Don Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Bondarenko
T I
TI
Miliutina
N P
NP
Mikhaleva
I I
II
D000818:Animals; D001921:Brain / Q000201:enzymology; D003701:Delta Sleep-Inducing Peptide / Q000502:physiology*; D004910:Erythrocyte Membrane / Q000201:enzymology / Q000502:physiology*; D005609:Free Radicals; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology; D009195:Peroxidase / Q000097:blood / Q000378:metabolism; D051381:Rats; D013312:Stress, Physiological / Q000201:enzymology / Q000503:physiopathology*
D005609:Free Radicals; D003701:Delta Sleep-Inducing Peptide; D009195:Peroxidase; D005979:Glutathione Peroxidase