title: Multiplex PCR for detection of aminoglycoside resistance genes in enterococci.
abstract: A multiplex PCR procedure for detecting the aminoglycoside resistance genes aac(6')-Ie-aph(2")-Ia, aph(2")-Ib, aph(2")-Ic, aph(2")-Id, aph(3')-IIIa, and ant(4')-Ia was evaluated and found to determine accurately the presence of these genes in enterococci.
authors.affiliation: Division of Infectious Diseases, Department of Biochemistry and Molecular Biology, Wayne State University, Detroit, Michigan 48201, USA.
authors.forename: Sergei B
authors.initials: SB
authors.lastname: Vakulenko
authors.forename: Susan M
authors.initials: SM
authors.lastname: Donabedian
authors.forename: Anatoliy M
authors.initials: AM
authors.lastname: Voskresenskiy
authors.forename: Marcus J
authors.initials: MJ
authors.lastname: Zervos
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Lerner
authors.forename: Joseph W
authors.initials: JW
authors.lastname: Chow
mesh_terms: D000617:Aminoglycosides; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016983:Enterococcus / Q000187:drug effects* / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents