[Effects of polyamine-loaded liposomes on liver mitochondrial permeability transition].
The objective of the present study was represented by the effects of polyamineloaded liposomes on hepatic mitochondrial permeability transition (MPT). MPT was appreciated through the swelling of the isolated guinea-pig liver mithocondria induced by Ca2+, at 540 nm, using a diode-array 8452 UV-VIS spectrophotometer and a General Purpose software (Hewlett-Packard). Polyamines encapsulated in liposomes might inhibit the appearance of MPT induced by Ca2+, directly proportional to electrical charge: spermine > spermidine > putrescine > cadaverine. Thus, together with some previous data, it was observed that spermine may modulate hepatic MPT from the exterior, as well as from the interior of mitochondria. Moreover, spermidine and putrescine may also modulate MPT from the interior of mitochondria at low doses, and from the exterior only at high doses. The changes in mitochondrial membranes lipid composition (as done by control liposomes) are slightly influencing the MPT. The increase in membrane rigidity through the use of 40% cholesterol-enriched liposomes is drastically decreasing the appearance and development of MPT.
Costuleanu
M
M
Facultatea de Medicină Stomatologică, Universitatea de Medicină şi Farmacie Gr.T. Popa Iaşi.
Foia
Liliana
L
Costuleanu
Natalia
N
Indrei
Anca
A
Slătineanu
Simona Mihaela
SM
Petrescu
Gh
G
D000818:Animals; D015317:Biogenic Polyamines / Q000493:pharmacokinetics*; D002463:Cell Membrane Permeability / Q000502:physiology*; D003196:Computer Graphics; D006168:Guinea Pigs; D008081:Liposomes / Q000493:pharmacokinetics*; D008930:Mitochondria, Liver / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D013053:Spectrophotometry; D013096:Spermine / Q000378:metabolism
D015317:Biogenic Polyamines; D008081:Liposomes; D013096:Spermine; D011700:Putrescine