2P1, a novel male mouse cDNA specifically expressed during meiosis.
We screened a mouse germinal cell expression library with a probe derived from Sob1, a human testis-specific cDNA, and identified 2P1, a new mouse cDNA. A database search revealed that 2P1 was 91% identical to ORF1 of E3-3, a rat gene probably involved in the regulation of alternative splicing. Sequencing showed that 2P1 has a destabilization motif in its 3'-untranslated region. Northern blotting showed strong gene expression in the testis and weak expression in the epididymis, with no signal detected in other tissues. RT-PCR analysis confirmed testis and epididymis expression. In situ hybridization revealed that 2P1 mRNA was absent in spermatogonia but expressed in spermatocytes. This last result was confirmed by RT-PCR of FACS isolated primary spermatocytes (pachytene stage). Using RT-PCR, purified spermatids were also shown to express 2P1.
Hammami-Hamza
Sonia
S
INSERM U566/CEA/Université Paris 7, Gamétogenèse et Génotoxicité, Fontenay-aux-roses, Paris, France.
Doussau
Mireille
M
Allemand
Isabelle
I
Segretain
Dominique
D
Gasc
Jean-Marie
JM
Finaz
Catherine
C
D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005854:Germ Cells / Q000166:cytology; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008540:Meiosis; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013087:Spermatids / Q000502:physiology*; D013090:Spermatocytes / Q000502:physiology*; D013091:Spermatogenesis; D013737:Testis / Q000502:physiology
D054758:A Kinase Anchor Proteins; C119530:AKAP3 protein, human; D048868:Adaptor Proteins, Signal Transducing; C485611:Akap3 protein, mouse; D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger