title: Development of microsatellite markers from an ectomycorrhizal fungus, Tricholoma matsutake, by an ISSR-suppression-PCR method.
abstract: An inter-simple sequence repeat (ISSR)-suppression-PCR technique established to develop microsatellite markers of plant species was applied to an ectomycorrhizal fungus, Tricholoma matsutake. Six polymorphic SSR markers were developed. All six polymorphic SSR markers were single-locused and co-dominant. Alleles produced by these six single-locused markers ranged from two to nine per locus and the expected heterozygosities were calculated as values from 0.098 to 0.803. The results indicated that the ISSR-suppression-PCR technique was effective and applicable to the development of microsatellite markers from ectomycorrhizal fungi. Furthermore, the six microsatellite loci did not amplify DNA from any other ectomycorrhizal species investigated, except for Tricholoma nauseosum (Swedish matsutake) and Tricholoma fulvocastaneum, suggesting that population genetics and reproduction of T. matsutake could be investigated by the SSR markers developed in the present study.
authors.affiliation: Asian Natural Environmental Science Center, The University of Tokyo, Midori-cho 1-1-8, Nishitokyo-shi, Tokyo 188-0002, Japan. lian@uf.a.u-tokyo.ac.jp
authors.forename: Chunlan
authors.initials: C
authors.lastname: Lian
authors.forename: Taizo
authors.initials: T
authors.lastname: Hogetsu
authors.forename: Norihisa
authors.initials: N
authors.lastname: Matsushita
authors.forename: Alexis
authors.initials: A
authors.lastname: Guerin-Laguette
authors.forename: Kazuo
authors.initials: K
authors.lastname: Suzuki
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Yamada
mesh_terms: D001487:Basidiomycota / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D005787:Gene Frequency / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D038821:Mycorrhizae / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D004271:DNA, Fungal