title: Preparation and comparative analysis of two podophyllotoxin liposomes.
abstract: OBJECTIVE
  To prepare two different liposome gels containing podophyllotoxin (PPT) and compare their pharmaceutical properties.
  
  
  METHODS
  PPT-Dipalmitoylphosphatidylcholine (DPPC) liposome and PPT-soya lecithin liposome were prepared by reverse phase evaporation technique, and optical and electron microscopy were performed respectively for examining the appearances of the 2 liposomes and the diameter of the particles. Gel filtration was employed to assess the embedding ratio of the liposomes.
  
  
  RESULTS
  Under optical microscope, PPT-DPPC liposome was observed to be composed of spherical particles with diameter range of 2.05 to 0.825 microm (average 1.45 microm). The diameter range of the particles of PPT-soya lecithin liposome is 7.59 to 1.445 microm (average 3.78 microm). PPT-DPPC liposome appeared multivesicular under electron microscope, with the embedding ratio of 73.8%, and 79.1% for PPT-soya lecithin liposome (n=3). PPT-DPPC liposome had a embedding ratio of 65.2%, 58.8% and 56.4% after preserved 1, 3, 6 months respectively. As for PPT-soya lecithin liposome, the embedding ratios were 70.3%, 60.4%, and 51.3% respectively.
  
  
  CONCLUSIONS
  The PPT in the liposomes prepared by reverse phase evaporation technique is evenly distributed throughout the gel. The preparation is relatively simple with high embedding ratio.
authors.affiliation: Department of Dermatology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Gui-rong
authors.initials: GR
authors.lastname: Hou
authors.forename: Kang
authors.initials: K
authors.lastname: Zeng
authors.forename: Zai-gao
authors.initials: ZG
authors.lastname: Zhou
authors.forename: Guo-feng
authors.initials: GF
authors.lastname: Li
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D008081:Liposomes; D010713:Phosphatidylcholines / Q000737:chemistry*; D011034:Podophyllotoxin / Q000008:administration & dosage*; D013025:Glycine max / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D008081:Liposomes; D010713:Phosphatidylcholines; D015060:1,2-Dipalmitoylphosphatidylcholine; D011034:Podophyllotoxin