title: Production of IL-12 and IL-18 in human dendritic cells upon infection by Listeria monocytogenes.
abstract: Dendritic cells (DCs) are major antigen-presenting cells of the immune system, which need to be activated in order to initiate an immune response. Here, we describe the immunostimulatory effects on human monocyte-derived DCs observed upon infection with Listeria monocytogenes or after treatment with listerial lipoteichoic acid (LTA) and lipopolysaccharide (LPS), respectively. All stimuli caused upregulation of costimulatory molecules, induced T-cell proliferative responses and secretion of cytokines in vitro. Infection of DCs with L. monocytogenes induced release of interleukin (IL)-12 and IL-18. In contrast treatment with purified listerial LTA yielded high levels of IL-18 release, but only minimal IL-12 production. Treatment of DCs with LPS conversely induced significant amounts of IL-12 production, but no IL-18. The release of both stimulating cytokines IL-12 and IL-18 upon infection with entire bacteria suggests that attenuated strains of L. monocytogenes may be a valuable tool for subunit vaccine delivery.
authors.affiliation: Department of Dermatology, University of Würzburg, 97080 Würzburg, Germany. ankolb@biozentrum.uni-wuerzburg.de
authors.forename: Annette
authors.initials: A
authors.lastname: Kolb-Mäurer
authors.forename: Ulrike
authors.initials: U
authors.lastname: Kämmerer
authors.forename: Mathias
authors.initials: M
authors.lastname: Mäurer
authors.forename: Ivaylo
authors.initials: I
authors.lastname: Gentschev
authors.forename: Eva Bettina
authors.initials: EB
authors.lastname: Bröcker
authors.forename: Peter
authors.initials: P
authors.lastname: Rieckmann
authors.forename: Eckhart
authors.initials: E
authors.lastname: Kämpgen
mesh_terms: D000328:Adult; D001428:Bacterial Vaccines; D002454:Cell Differentiation; D002478:Cells, Cultured / Q000378:metabolism / Q000382:microbiology; D003713:Dendritic Cells / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D005786:Gene Expression Regulation; D006801:Humans; D018664:Interleukin-12 / Q000096:biosynthesis* / Q000235:genetics; D020382:Interleukin-18 / Q000096:biosynthesis* / Q000235:genetics; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000737:chemistry / Q000502:physiology*; D007959:Lymphocyte Culture Test, Mixed; D009000:Monocytes / Q000166:cytology / Q000187:drug effects; D013682:Teichoic Acids / Q000302:isolation & purification / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics; D014613:Vaccines, Attenuated; D022223:Vaccines, Subunit
chemical_list: D001428:Bacterial Vaccines; D020382:Interleukin-18; D008070:Lipopolysaccharides; D013682:Teichoic Acids; D014409:Tumor Necrosis Factor-alpha; D014613:Vaccines, Attenuated; D022223:Vaccines, Subunit; D018664:Interleukin-12; C009900:lipoteichoic acid