Lack of biofilm contribution to bacterial colonisation in an experimental model of foreign body infection by Staphylococcus aureus and Staphylococcus epidermidis.
The contribution of in vivo biofilm-forming potential of Staphylococcus aureus and Staphylococcus epidermidis was studied in an experimental model of foreign body infections. Increasing inocula (from 10(2) to 10(7) organisms) of ica-positive strains of S. aureus and S. epidermidis and their ica-negative isogenic mutants (the ica locus codes for a major polysaccharide component of biofilm) were injected into subcutaneously implanted tissue cages in guinea pigs. Surprisingly, bacterial counts and time-course of tissue cage infection by ica-positive strains of S. aureus or S. epidermidis were equivalent to those of their respective ica-negative mutants, in the locally infected fluids and on tissue-cage-inserted plastic coverslips.
Francois
Patrice
P
Division of Infectious Diseases, University Hospitals of Geneva, 1211 Geneva 14, Switzerland. patrice.francois@hcuge.ch
Tu Quoc
Patrick H
PH
Bisognano
Carmelo
C
Kelley
William L
WL
Lew
Daniel P
DP
Schrenzel
Jacques
J
Cramton
Sarah E
SE
Götz
Friedrich
F
Vaudaux
Pierre
P
D000818:Animals; D018441:Biofilms; D004195:Disease Models, Animal; D005547:Foreign Bodies / Q000150:complications*; D006168:Guinea Pigs; D013203:Staphylococcal Infections / Q000209:etiology*; D013211:Staphylococcus aureus / Q000472:pathogenicity*; D013212:Staphylococcus epidermidis / Q000472:pathogenicity*