title: Molecular characterization of the prototrophic Salmonella mutants defective for intraepithelial replication.
abstract: Three MudJ prototrophs demonstrated that intracellular replication is a Salmonella virulence trait (K. Y. Leung and B. B. Finlay, Proc. Natl. Acad. Sci. USA, 88:11470-11474, 1991). mutS and mutH are disrupted in mutants 3-11 and 12-23, and ssaQ is disrupted in mutant 17-21. Further analysis revealed that loss of Salmonella pathogenicity island 2 function underlies the intracellular replication defect of 3-11 and 17-21.
authors.affiliation: Department of Microbiology & Molecular Genetics, University of Vermont, 95 Carrigan Drive, Burlington, VT 05405, USA.
authors.forename: Akamol E
authors.initials: AE
authors.lastname: Suvarnapunya
authors.forename: Daniel V
authors.initials: DV
authors.lastname: Zurawski
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Guy
authors.forename: Murry A
authors.initials: MA
authors.lastname: Stein
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D004268:DNA-Binding Proteins; D004847:Epithelial Cells / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D051719:MutS DNA Mismatch-Binding Protein; D009154:Mutation; D012475:Salmonella / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C440638:SPI-2 protein, Salmonella; D000251:Adenosine Triphosphatases; D051719:MutS DNA Mismatch-Binding Protein; C497175:MutS protein, E coli