title: Nucleotide sequence and genome organization of Cucumber yellows virus, a member of the genus Crinivirus.
abstract: The genome of Cucumber yellows virus (CuYV), isolated in Japan from cucumber (Cucumis sativus L.), was completely sequenced and shown to be bipartite. CuYV RNA1 consisted of 7889 nucleotides and encompassed seven open reading frames (ORFs), which is typical of the Closteroviridae, including a heat-shock protein 70 homologue, a coat protein and a diverged coat protein (CPd). CuYV RNA2 consisted of 7607 nucleotides and included two ORFs: ORF1a potentially encoded a polyprotein containing putative papain-like protease, methyltransferase and helicase domains, and ORF 1b potentially encoded an RNA-dependent RNA polymerase, which is probably expressed via a +1 ribosomal frameshift. The size and organization of the CuYV genome are similar to those of Lettuce infectious yellows virus (LIYV), the type member of the genus Crinivirus in the family Closteroviridae, indicating that CuYV is a member of that genus, although CuYV differed in several points from LIYV.
authors.affiliation: Faculty of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya 321-8505, Japan.
authors.forename: Sedyo
authors.initials: S
authors.lastname: Hartono
authors.affiliation: Faculty of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya 321-8505, Japan.
authors.forename: Tomohide
authors.initials: T
authors.lastname: Natsuaki
authors.affiliation: Nihon Horticultural Production Institute, Kamishiki 207, Matsudo 270-2221, Japan.
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Genda
authors.affiliation: Faculty of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya 321-8505, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Okuda
mesh_terms: D001483:Base Sequence; D029383:Crinivirus / Q000737:chemistry / Q000235:genetics*; D018553:Cucumis sativus / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D016415:Sequence Alignment