title: A fully coupled binary biochemical reactive-diffusion model with analytic solution.
abstract: Coupled multicomponent biochemical reactive diffusion underlies a variety of biological signalling processes and pharmacokinetic applications, such as paracrine signalling involving "cocktails" comprised of growth promoter/inhibitor factors and proteases associated with tumor angiogenesis, invasion and metastasis, extravascular drug delivery, and polymeric controlled-release drug codelivery design. Here, we present a model and develop a new analytic solution to illustrate the spatiotemporal behavior associated with fully coupled binary biochemical reactive diffusion. The complete coupling renders the solution appreciably more complex in structure and behavior than solutions for unicomponent or partially coupled models. Concentration behavior is illustrated by the computational simulation of binary-species tumor angiogenesis factor reactive-diffusion in the extravascular tissue matrix. The computational results indicate that (a) steady-state concentration profiles are achieved within 1 h of a change in factor production; (b) in the steady state, the spatial profiles of the two components tend to be similar; (c) exceedingly steep concentration gradients, involving several orders-of-magnitude differences in concentration over a few tenths of a millimeter, can occur in the vicinity of boundary sources due to inter-species reaction; (d) the concentration profiles of the two species differ from unicomponent predictions due to the simultaneous mass interchange between the two species. The analytic solution predictions are also used to provide a first-ever validation of a time-dependent, binary-component Crank-Nicholson numerical solution. The ability to quantitatively model interacting and often strongly varying concentration levels as a function of time and position can serve as a powerful complementary tool to experimental analyses for assessing disease state and interventional pharmacological efficacy, especially when the spatial scales on which in vivo behavior occurs taxes the limits of imaging capabilities.
authors.affiliation: Department of Radiology, The University of Texas, Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA. ligenev@netscape.net
authors.forename: J W
authors.initials: JW
authors.lastname: Durkee
authors.forename: P P
authors.initials: PP
authors.lastname: Antich
authors.forename: M A
authors.initials: MA
authors.lastname: Lewis
authors.forename: R W
authors.initials: RW
authors.lastname: Parkey
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D019295:Computational Biology; D004058:Diffusion; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism; D008954:Models, Biological; D019899:Paracrine Communication / Q000502:physiology*; D013997:Time Factors
chemical_list: D043925:Angiogenesis Inducing Agents