Spectroimmunochemistry using colloidal gold bioconjugates.
Using surface-enhanced infrared absorption (SEIRA) spectroscopy of dry films of colloidal gold (CG) bioconjugates with protein A, it is shown that certain characteristic bands of the protein (e.g., amide I, amide II and some other vibration modes) are essentially affected by the metal surface. Thus, the method may be used for controlling the quality of such bioconjugates. Moreover, it is demonstrated that the biospecific reaction of protein A attached to CG particles with human immunoglobulin G (IgG) results in further essential changes in SEIRA spectra, providing a means for an easy and rapid IR spectroscopic detection of biospecific immunochemical interactions (i.e., spectroimmunochemistry). The results obtained can form a basis for developing test systems for detecting various biospecific interactions.
Kamnev
Alexander A
AA
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prosp. Entuziastov, 410015 Saratov, Russia. aakamnev@ibppm.sgu.ru
Dykman
Lev A
LA
Tarantilis
Petros A
PA
Polissiou
Moschos G
MG
D001665:Binding Sites / Q000502:physiology; D017979:Gold Colloid / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D007150:Immunohistochemistry / Q000379:methods*; D008968:Molecular Conformation; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013205:Staphylococcal Protein A / Q000032:analysis*
D017979:Gold Colloid; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A