Coupled column chromatography for separation and determination of enantiomers of phenylcarbamic acid derivatives in serum.
Columns packed with vancomycin coupled to an achiral C18 column and beta-cyclodextrin were used for the separation and the determination of enantiomers of alkoxysubstituted esters of phenylcarbamic acid in blood serum. The method involves off-line SPE, the separation of the racemate on a reversed-phase stationary phase, and the separation of the enantiomers on a chiral stationary phase. The limit of detection was 1.0 microg/ml for the vancomycin column and 10.0 microg/ml for the beta-cyclodextrin column in standard solution. In vitro degradation studies of enantiomers demonstrated a difference in the concentation of the enantiomers after the treatment. It was found that the rate constants of R(-)- and S(+)-forms of enantiomers are not significantly different.
Rojkovicová
T
T
Department of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak Technical University, Bratislava, Slovak Republic.
Hrobonová
K
K
Lehotay
J
J
Cizmárik
J
J
D002219:Carbamates / Q000097:blood* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003505:Cyclodextrins; D007202:Indicators and Reagents; D007700:Kinetics; D013237:Stereoisomerism; D017334:Teicoplanin / Q000097:blood; D014640:Vancomycin / Q000097:blood; D047392:beta-Cyclodextrins
D002219:Carbamates; D003505:Cyclodextrins; D007202:Indicators and Reagents; D047392:beta-Cyclodextrins; D017334:Teicoplanin; D014640:Vancomycin; C031215:betadex; C059692:phenylcarbamic acid