title: Measurement of phosphatidylcholine hydroperoxide in mild ischemia-reperfusion injury in rat intestine.
abstract: In the pathogenesis of intestinal ischemia-reperfusion injury, the measurement of lipid peroxides needs to be established. Sprague-Dawley rat intestines were assessed after 30 minutes of occlusion of the superior mesenteric artery followed by reperfusion at 30, 60, 120, 180, 360 minutes. Grade of the mucosal injury, accumulation of the activated neutrophils and ICAM-1 expression were transiently increased after reperfusion. Two measuring methods of mucosal lipid peroxides using thiobarbituric acid reacting substance (TBARS) and phosphatidylcholine hydroperoxide (PCOOH) were compared. PCOOH level was significantly increased after reperfusion, while the mucosal TBARS level showed no significant change. In conclusion, lipid peroxidation could be detected with high specificity and sensitivity by measuring the mucosal phosphatidylcholine hydroperoxide level.
authors.affiliation: Department of Surgery, Division of GI and Colorectal Surgery, Research Unit of Biological Regulation and Oncology, Tohoku University, Graduate School of Medicine, Sendai 980-8574, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Masuko
authors.forename: Yuji
authors.initials: Y
authors.lastname: Funayama
authors.forename: Hiroo
authors.initials: H
authors.lastname: Naito
authors.forename: Kohei
authors.initials: K
authors.lastname: Fukushima
authors.forename: Chikashi
authors.initials: C
authors.lastname: Shibata
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Takahashi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ogawa
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Ueno
authors.forename: Akihiko
authors.initials: A
authors.lastname: Hashimoto
authors.forename: Teruo
authors.initials: T
authors.lastname: Miyazawa
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Seiki
authors.initials: S
authors.lastname: Matsuno
authors.forename: Iwao
authors.initials: I
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009504:Neutrophils / Q000473:pathology; D010713:Phosphatidylcholines / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D012680:Sensitivity and Specificity; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
chemical_list: D010713:Phosphatidylcholines; D017392:Thiobarbituric Acid Reactive Substances; C057590:phosphatidylcholine hydroperoxide; D018799:Intercellular Adhesion Molecule-1