Modulation of the NO trans effect in heme proteins: implications for the activation of soluble guanylate cyclase.
The binding of NO to the iron heme in guanylate cyclase and other heme proteins induces the cleavage of the proximal histidine bonded to the metal. In this study we assess by means of density functional theory (DFT) electronic structure calculations the role of H-bonding to histidine in the modulation of this effect. We have considered in the first place a model of the isolated active site coordinated with imidazole and imidazolate to mimic the effects of a very strong H-bond. We have also investigated four selected ferrous heme proteins with different proximal histidine environments: the O(2) sensing FixL, horseradish peroxidase C, and the alpha and beta subunits of human hemoglobin. Our results indicate that polarization and charge transfer effects associated with H-bonding to the proximal histidine play a fundamental role in the modulation of the NO trans effect in heme proteins. We also find computational evidence suggesting that protein structural constraints may affect significantly the cleavage of the Fe-His bond.
Martí
Marcelo A
MA
Departamento de Química Inorgánica, Analítica y Química Física and INQUIMAE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. II (C1428EHA), Buenos Aires, Argentina.
Scherlis
Damián A
DA
Doctorovich
Fabio A
FA
Ordejón
Pablo
P
Estrin
Darío A
DA
D001665:Binding Sites; D004789:Enzyme Activation; D006162:Guanylate Cyclase / Q000378:metabolism*; D006418:Heme / Q000737:chemistry / Q000378:metabolism; D006420:Hemeproteins / Q000737:chemistry* / Q000378:metabolism*; D006639:Histidine / Q000378:metabolism; D006801:Humans; D006860:Hydrogen Bonding; D007093:Imidazoles / Q000378:metabolism; D008956:Models, Chemical; D009569:Nitric Oxide / Q000378:metabolism*; D012995:Solubility
D006420:Hemeproteins; D007093:Imidazoles; D009569:Nitric Oxide; D006418:Heme; D006639:Histidine; D006162:Guanylate Cyclase