title: In vitro cytocompatibility studies of Diamond Like Carbon coatings on titanium.
abstract: Diamond like carbon (DLC) films were deposited on to titanium (Ti) substrates by Plasma Enhanced Chemical Vapour Deposition (PECVD) process. The quality of the films were checked by Raman spectra and nano-hardness tests. The cytocompatibility of titanium and DLC coated titanium were studied using continuous cell lines of mouse fibroblast cells ( L-929), Human Osteoblast cells (HOS) and primary human umbilical cord vein endothelial cells (HUVEC). The cellular responses to the materials were assessed both quantitatively and qualitatively. The adhesion and spreading of cells on materials were compared using Ti as a control. Present study indicates an improved cytocompatibility of DLC coated Ti in comparison to bare Ti.
authors.affiliation: BMT Wing, Sree Chitra Tirunal Institute of Medical Sciences & Technology, Poojapura, Thiruvananthapuram, Kerala 695 012, India. tvkumari@sctimst.ker.nic.in
authors.forename: T V
authors.initials: TV
authors.lastname: Kumari
authors.forename: P R
authors.initials: PR
authors.lastname: Anil Kumar
authors.forename: C V
authors.initials: CV
authors.lastname: Muraleedharan
authors.forename: G S
authors.initials: GS
authors.lastname: Bhuvaneshwar
authors.forename: Y
authors.initials: Y
authors.lastname: Sampeur
authors.forename: F
authors.initials: F
authors.lastname: Derangere
authors.forename: R
authors.initials: R
authors.lastname: Suryanarayanan
mesh_terms: D000818:Animals; D002244:Carbon / Q000009:adverse effects / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000009:adverse effects* / Q000737:chemistry*; D018130:Diamond / Q000009:adverse effects* / Q000737:chemistry*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000648:ultrastructure; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure; D006244:Hardness; D006801:Humans; D008422:Materials Testing / Q000379:methods; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000648:ultrastructure; D013059:Spectrum Analysis, Raman; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D002244:Carbon; D018130:Diamond; D014025:Titanium