title: Elevated serum levels of thioredoxin in patients with acute exacerbation of asthma.
abstract: The pathogenesis of bronchial asthma is chronic airway inflammation caused by immune cells such as T lymphocytes and eosinophils. Eosinophils release cytotoxic products including reactive oxygen species at the site of inflammation, leading to epithelial damage. Human thioredoxin (TRX), a redox-regulating protein with antioxidant activity, is induced and secreted from cells by oxidative stress. This study was undertaken to investigate the clinical significance of TRX in the pathogenesis of asthma. We collected blood samples from 48 patients with bronchial asthma with or without attack, and measured serum ECP and pulmonary function as well as serum TRX. The serum TRX levels in patients with asthma were significantly increased in patients with mild (34.63 [28.40-42.73] ng/ml, medians with 25 and 75% interquartiles, P=0.0064) and moderate (38.83 [35.14-50.80] ng/ml, P=0.0017) asthma attacks compared with those during the asymptomatic period. The serum TRX levels were inversely correlated with FEV(1.0)% (r=-0.44, P=0.039) and %PEF (r=-0.49, P=0.020) during attack. There was a significant correlation between the serum TRX and the serum eosinophil cationic protein (rs=0.32, P=0.016). These findings suggest that serum TRX is related to the state of asthma exacerbation and allergic inflammation.
authors.affiliation: Department of Clinical and Laboratory Medicine, Akita University School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Yamada
authors.forename: Hajime
authors.initials: H
authors.lastname: Nakamura
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Adachi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sannohe
authors.forename: Hajime
authors.initials: H
authors.lastname: Oyamada
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kayaba
authors.forename: Junji
authors.initials: J
authors.lastname: Yodoi
authors.forename: Junichi
authors.initials: J
authors.lastname: Chihara
mesh_terms: D001249:Asthma / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D001798:Blood Proteins / Q000032:analysis; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012129:Respiratory Function Tests; D012260:Ribonucleases / Q000032:analysis / Q000097:blood; D012907:Smoking; D013879:Thioredoxins / Q000097:blood*
chemical_list: D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D013879:Thioredoxins; D012260:Ribonucleases