title: Afa/Dr diffusely adhering Escherichia coli infection in T84 cell monolayers induces increased neutrophil transepithelial migration, which in turn promotes cytokine-dependent upregulation of decay-accelerating factor (CD55), the receptor for Afa/Dr adhesins.
abstract: Ulcerative colitis and Crohn's disease are inflammatory bowel diseases thought to involve strains of Escherichia coli. We report here that two wild-type Afa/Dr diffusely adhering E. coli (DAEC) strains, C1845 and IH11128, which harbor the fimbrial F1845 adhesin and the Dr hemagglutinin, respectively, and the E. coli laboratory strain HB101, transformed with the pSSS1 plasmid to produce Afa/Dr F1845 adhesin, all induced interleukin-8 (IL-8) production and transepithelial migration of polymorphonuclear leukocytes (PMNL) in polarized monolayers of the human intestinal cell line T84 grown on semipermeable filters. We observed that after PMNL migration, expression of decay-accelerating factor (DAF, or CD55), the brush border-associated receptor for Afa/Dr adhesins, was strongly enhanced, increasing the adhesion of Afa/Dr DAEC bacteria. When examining the mechanism by which DAF expression was enhanced, we observed that the PMNL transepithelial migration induced epithelial synthesis of tumor necrosis factor alpha and IL-1beta, which in turn promoted the upregulation of DAF.
authors.affiliation: Unité INSERM 36, IFR 50, Faculté de Médecine, avenue de Valombrose, 06107 Nice Cédex 02, France.
authors.forename: Fréderic
authors.initials: F
authors.lastname: Bétis
authors.forename: Patrick
authors.initials: P
authors.lastname: Brest
authors.forename: Véronique
authors.initials: V
authors.lastname: Hofman
authors.forename: Julie
authors.initials: J
authors.lastname: Guignot
authors.forename: Imad
authors.initials: I
authors.lastname: Kansau
authors.forename: Bernard
authors.initials: B
authors.lastname: Rossi
authors.forename: Alain
authors.initials: A
authors.lastname: Servin
authors.forename: Paul
authors.initials: P
authors.lastname: Hofman
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D001422:Bacterial Adhesion; D018958:CD55 Antigens / Q000378:metabolism*; D016764:Cell Polarity; D016207:Cytokines / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006388:Hemagglutinins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008854:Microscopy, Electron; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000276:immunology; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
chemical_list: D018830:Adhesins, Escherichia coli; D018958:CD55 Antigens; D016207:Cytokines; C465564:Dr adhesin, E coli; D029968:Escherichia coli Proteins; D006388:Hemagglutinins