In vitro proliferation of blood, spleen and thymus leukocytes from the turtle Mauremys caspica.
Cell-mediated immune responses is commonly evaluated by cell proliferation assays. Mitogens are known to induce a vigorous proliferative response in lymphoid cells from mammals but relatively fewer studies have investigated mitogen-mediated lymphoproliferation in non-mammalian animals. In the present work, we incubated spleen, thymus and blood leukocytes with phytohaemagglutinin (PHA), concanavalin A (Con A), lipopolysaccharide (LPS) and pokeweed mitogen (PWM), by different times of incubation (96 and 120 h) and at different concentrations. Our results show that the optimal mitogen concentrations inducing proliferation on leukocytes from Mauremys caspica were 20 microg/ml PHA, 1 microg/ml Con A, 12.5 microg/ml LPS and 1/150 dilution PWM. The optimal time of incubation was dependent on the type of leukocytes (peripheral blood leukocytes, splenic leukocytes or thymic cells) and the mitogen utilized.
Muñoz
Francisco J
FJ
Cell Signalling Unit, Department of Experimental and Health Sciences, Faculty of Health and Life Sciences, Pompeu Fabra University, Barcelona 08003, Spain. paco.munoz@cexs.upf.es
De la Fuente
Mónica
M
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D008214:Lymphocytes / Q000097:blood / Q000166:cytology / Q000187:drug effects*; D008297:Male; D008934:Mitogens / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology*; D014426:Turtles / Q000097:blood / Q000276:immunology*
D008934:Mitogens