title: Oxidative damage of sulfur dioxide inhalation on stomachs and intestines of mice.
abstract: Effects of exposure to sulfur dioxide (SO(2)) on levels of thiobarbituric acid-reactive substances (TBARS), levels of reduced glutathione (GSH), and the activities of Cu,Zn-superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were investigated in stomachs and intestines of Kunming albino mice of both sexes. SO(2) exposure at different concentrations (22, 56, and 112 mg/m(3)) was administered to the animals of SO(2) groups in the exposure chambers for 6 h/day for 7 days, while control groups were exposed to filtered air under the same condition. Our results show that SO(2) caused lipid peroxidation and changes of antioxidative status in stomachs and intestines of mice. Exposure to SO(2) at all concentrations tested caused significantly the increase of TBARS levels in stomachs and intestines of mice. For the stomachs, activities of these antioxidant enzymes and levels of GSH were significantly unaltered by SO(2) at low concentrations, except significant increase of SOD activity in the stomachs of male mice. However, higher SO(2) caused the significant increases of CAT activities and the significant decreases of GSH levels and activities of SOD and GPx. For intestines, SO(2) at all concentrations tested decreased significantly activities of SOD relative to control animals; SO(2) caused the decreases of GPx activities, but only the decreases of GPx activities caused by SO(2) exposures at higher concentrations (56 and 112 mg/m(3)) were statistically significant. SO(2) at all concentrations tested tended to increase the CAT activities in a dose-dependent manner, but the decreases of CAT activities caused by higher SO(2) were significant. SO(2) at all concentrations tested decreased significantly levels of GSH in intestines of both sexual mice. These results lead to conclusion that SO(2) exposure can caused oxidative damage to stomachs and intestines of mice, and SO(2) is a toxic agent to stomachs and intestines of mammals, not only to respiratory system. Further work is required to understand toxicological role of SO(2) on multiple or even all organs in human and animal.
authors.affiliation: Institute of Environmental Medicine and Toxicology, Department of Life Sciences, Shanxi University, Taiyuan, 030006, China. zqmeng@sxu.edu.cn
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Meng
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Juli
authors.initials: J
authors.lastname: Bai
authors.forename: Hong
authors.initials: H
authors.lastname: Geng
authors.forename: Chengyun
authors.initials: C
authors.lastname: Liu
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D002374:Catalase / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005260:Female; D005978:Glutathione / Q000032:analysis; D005979:Glutathione Peroxidase / Q000032:analysis; D007422:Intestines / Q000737:chemistry / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D011506:Proteins / Q000032:analysis; D013270:Stomach / Q000737:chemistry / Q000187:drug effects*; D013458:Sulfur Dioxide / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000032:analysis; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D000975:Antioxidants; D011506:Proteins; D017392:Thiobarbituric Acid Reactive Substances; D013458:Sulfur Dioxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione