Expression of MIP-3alpha/CCL20, a macrophage inflammatory protein in oral squamous cell carcinoma.
We have examined the expression of MIP-3alpha/CCL20 in oral squamous cell carcinoma (SCC) in vivo and in vitro. In addition, we have investigated whether the expression of MIP-3alpha/CCL20 is regulated by bacterial infection and inflammatory cytokines. In order to determine the mRNA level of MIP-3alpha, quantitative reverse transcription-polymerase chain reaction (RT-PCR) was performed with LightCycler using the double-stranded DNA dye, SYBR Green I. Oral epithelial cells and six SCC cell lines (SCC-9, SAS, BSC-OF, HSC-4, HSC, Ca9-22) were found to express MIP-3alpha mRNA. The expression of MIP-3alpha was upregulated by infection with Actinobacillus actinomycetemcomitans and by stimulation with lipopolysaccharide and TNF-alpha. By in situ hybridization, the detectable MIP-3alpha expression in SCC was localized primarily at the epithelial pearls corresponding to the spinous layer. These results suggest that MIP-3alpha contributes to the oral immunoresponse to bacterial infection, and may be involved in the growth of SCC.
Abiko
Yoshihiro
Y
Department of Oral Pathology, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan. yoshi-ab@hoku-iryo-u.ac.jp
Nishimura
Michiko
M
Kusano
Kaoru
K
Nakashima
Keisuke
K
Okumura
Kazuhiko
K
Arakawa
Toshiya
T
Takuma
Taishin
T
Mizoguchi
Itaru
I
Kaku
Tohru
T
D016976:Aggregatibacter actinomycetemcomitans; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000378:metabolism*; D054418:Chemokine CCL20; D019742:Chemokines, CC / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D019402:Macrophage Inflammatory Proteins / Q000276:immunology / Q000378:metabolism*; D009062:Mouth Neoplasms / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D054399:Receptors, CCR6; D019707:Receptors, Chemokine; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
C108355:CCL20 protein, human; C106311:CCR6 protein, human; D054418:Chemokine CCL20; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger; D054399:Receptors, CCR6; D019707:Receptors, Chemokine; D014409:Tumor Necrosis Factor-alpha