title: Effects of X-irradiation in early ontogenesis on the longevity and amount of the S1 nuclease-sensitive DNA sites in adult Drosophila melanogaster.
abstract: The long-term consequences of the X-irradiation of Drosophila melanogaster fruit fly one-hour eggs with doses of 0.25, 0.50, 0.75, 1, 2 and 4 Gy were investigated. Longevity hormesis was observed in males exposed to 0.5 Gy and 0.75 Gy, but no longevity increase was observed in females. The electrophoretic analysis has shown that the amount of the DNA segments resulting from cleavage in S1 nuclease-sensitive sites (<3 kb) reached 39.2% of the total DNA from control males. DNA from the irradiated males had a smaller amount of such fragments (10-30% in different experimental groups). These findings indicate that the longevity hormesis may be associated with irradiation-induced long-term structural and/or functional DNA modifications.
authors.affiliation: Laboratory of Mathematical Modelling of Aging Processes, Institute of Gerontology, Vyshgorodskaya st. 67, Kiev 04114, Ukraine. vaiserman@geront.kiev.ua
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Vaiserman
authors.forename: Natalya M
authors.initials: NM
authors.lastname: Koshel
authors.forename: Alexander Y
authors.initials: AY
authors.lastname: Litoshenko
authors.forename: Tatyana G
authors.initials: TG
authors.lastname: Mozzhukhina
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Voitenko
mesh_terms: D000818:Animals; D004247:DNA / Q000378:metabolism*; D053938:DNA Fragmentation; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects; D005260:Female; D008136:Longevity / Q000502:physiology / Q000528:radiation effects*; D008297:Male; D010063:Ovum / Q000502:physiology / Q000528:radiation effects*; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism*
chemical_list: D004247:DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases