title: A structural and primary sequence comparison of the viral RNA-dependent RNA polymerases.
abstract: A systematic bioinformatic approach to identifying the evolutionarily conserved regions of proteins has verified the universality of a newly described conserved motif in RNA-dependent RNA polymerases (motif F). In combination with structural comparisons, this approach has defined two regions that may be involved in unwinding double-stranded RNA (dsRNA) for transcription. One of these is the N-terminal portion of motif F and the second is a large insertion in motif F present in the RNA-dependent RNA polymerases of some dsRNA viruses.
authors.affiliation: Department of Biological Sciences, State University of New York, Buffalo, NY 14260, USA. cambruen@nsm.buffalo.edu
authors.forename: Jeremy A
authors.initials: JA
authors.lastname: Bruenn
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D017124:Conserved Sequence / Q000235:genetics; D030561:Databases, Nucleic Acid; D008958:Models, Molecular; D012328:RNA Viruses / Q000201:enzymology / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment / Q000379:methods
chemical_list: D012324:RNA-Dependent RNA Polymerase