Two-dimensional protein map of human vastus lateralis muscle.
Protein samples of human vastus lateralis muscle were analyzed by two-dimensional gel electrophoresis, using immobilized pH gradients encompassing several pH regions in the first dimension and sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the second dimension. More than 500 protein spots on each gel were detected by silver staining, of which 150 were excised, digested in-gel with trypsin and characterized by matrix assisted laser desorptioin/ionization-mass spectrometry and tandem electrospray mass spectrometry. Using these techniques, 124 spots including contractile proteins and metabolic enzymes were identified. This database should provide a valuable resource for the definition of the functional properties of muscle fibres and for assessment of the influence of e.g., hypoxia, ischaemia and ageing on protein level.
Gelfi
Cecilia
C
IBFM, National Research Council, Segrate, Milan, Italy. gelfi@inb.mi.cnr.it
De Palma
Sara
S
Cerretelli
Paolo
P
Begum
Shajna
S
Wait
Robin
R
Begum
Shagn
S
D000293:Adolescent; D000328:Adult; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009124:Muscle Proteins / Q000302:isolation & purification*; D010449:Peptide Mapping / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D009124:Muscle Proteins