Clinical significance of heat shock protein-70 expression in bladder cancer.
OBJECTIVE
To investigate the expression of heat shock protein-70 (HSP-70) in transitional cell bladder cancer and correlate it with normal and inflammatory bladder tissue samples.


METHODS
We used a three-step avidin-biotin method to examine the localization of a rabbit anti-HSP-70 polyclonal antibody in normal (n = 10), inflammatory (n = 10), and malignant (n = 67) bladder tissue samples.


RESULTS
Fifty-eight percent of the bladder cancer samples and only 10% and 20% of the normal and inflammatory bladder tissue samples, respectively, expressed HSP-70. Localization of the antibody correlated with the grade (P <0.001), stage (P <0.001), and survival (P <0.05) of the patients with malignancy.


CONCLUSIONS
HSP-70 is frequently overexpressed by bladder cancer cells and could be used as biochemical marker in patients with bladder cancer.
Syrigos
Konstantinos N
KN
Laboratory of Molecular Oncology, 3rd Department of Internal Medicine, Sotiria General Hospital, Athens University School of Medicine, Athens, Greece.
Harrington
Kevin J
KJ
Karayiannakis
Anastasios J
AJ
Sekara
Eleutheria
E
Chatziyianni
Emmy
E
Syrigou
Ekaterini I
EI
Waxman
Jonathan
J
D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002278:Carcinoma in Situ / Q000175:diagnosis / Q000378:metabolism; D002295:Carcinoma, Transitional Cell / Q000175:diagnosis / Q000378:metabolism*; D003556:Cystitis / Q000378:metabolism*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism; D011379:Prognosis; D011817:Rabbits; D016019:Survival Analysis; D001743:Urinary Bladder / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis / Q000378:metabolism*
D014408:Biomarkers, Tumor; D018840:HSP70 Heat-Shock Proteins; D009363:Neoplasm Proteins