[Preparation and application of the monoclonal antibody against hepatoma-specific gamma-glutamyltransferase].
OBJECTIVE
To prepare a monoclonal antibody against hepatoma-specific gamma-glutamyltransferase (GGT-II) and study it's application.


METHODS
Two Bal B/C mice were immunized with pure GGT-II, then their spleen cells were separated and fused to SP 2/0 myeloma cells so as to make hybridoma cell strain which could yield monoclonal antibody against GGT-II. And it's effect of binding GGT-II was detected by competitive inhibitory enzyme linked-immunosorbance assay (ELISA).


RESULTS
A mouse hybridoma cell strain which could steadily secrete the monoclonal antibody against GGT-II was obtained and named 2G4F6B2. This monoclonal antibody belonged to IgG1 subclass and was specific to GGT-II, without cross-reaction to GGT-II. The result of detecting human serum GGT-II by ELISA with the monoclonal antibody accorded with that by polyacrylamide gradient electrophoresis.


CONCLUSIONS
The monoclonal antibody against GGT-II prepared in this study has high specificity and can be applied in clinic to detect human serum GGT-II.
Tang
Wei
W
Department of Infectious Disease, Affiliated Hospital of Nantong Medical College, Nantong 226001, China.
Wang
Xiao Ying
XY
Gao
Jian Ee
JE
Ji
He Ping
HP
Tao
Qi Min
QM
Ji
Ying
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000201:enzymology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008113:Liver Neoplasms / Q000175:diagnosis / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D005723:gamma-Glutamyltransferase / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D005723:gamma-Glutamyltransferase