title: Isolation and sequence analysis of the rat dihydrolipoamide succinyltransferase gene.
abstract: The dihydrolipoamide succinyltransferase (DLST) gene of the alpha-ketoglutarate dehydrogenase complex (alpha-KGDC) was isolated from a rat genomic DNA library and sequenced. This gene was composed of 15 exons and 14 introns like the human DLST gene. Sequence analysis of the promoter-regulatory region of the rat DLST gene-(Dlst) showed the possible presence of a CAAT box-sequence and of the sequences for an AP-2 site and three Sp1 sites, but no TATA box-sequence was evidenced. The nucleotide sequences of introns 1 and 4 of the rat Dlst were significantly homologous to those of introns 1 and 4 of the human DLST gene. The sequence analysis of the rat Dlst suggested that the exon coding for the E3- and/or E1-binding domain may have been lost from the gene during evolution in eukaryotic DLST, possibly after mitochondrial symbiosis because prokaryotic DLST possesses the E3- and/or E1-binding domain.
authors.affiliation: Department of Biochemistry, Kagoshima Women's Junior College, 1-59-1 Murasakibaru, Kagoshima 890-8565, Japan. nakano@jkajyo.ac.jp
authors.forename: Kyoko
authors.initials: K
authors.lastname: Nakano
authors.forename: Mariko
authors.initials: M
authors.lastname: Tanabe
authors.forename: Shiro
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Shigeo
authors.initials: S
authors.lastname: Ohta
authors.forename: Shihoko
authors.initials: S
authors.lastname: Suzuki
authors.forename: Masako
authors.initials: M
authors.lastname: Shimura
authors.forename: Sadayuki
authors.initials: S
authors.lastname: Matuda
mesh_terms: D000217:Acyltransferases / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D017208:Rats, Wistar; D017422:Sequence Analysis, DNA; D024363:Transcription Initiation Site
chemical_list: D000217:Acyltransferases; C021515:dihydrolipoamide succinyltransferase