title: Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling.
abstract: In S. cerevisiae cells undergoing anaphase, a ras-related GTPase, Tem1, is located on the spindle pole body that enters the daughter cell and activates a signal transduction pathway, MEN, to allow mitotic exit. MEN activation must be reversed after mitotic exit to reset the cell cycle in G1. We find that daughter cells activate an Antagonist of MEN pathway (AMEN) in part through induction of the Amn1 protein that binds directly to Tem1 and prevents its association with its target kinase Cdc15. Failure of Amn1 function results in defects of both the spindle assembly and nuclear orientation checkpoints and delays turning off Cdc14 in G1. Thus, Amn1 is part of a daughter-specific switch that helps cells exit from mitotic exit and reset the cell cycle.
authors.affiliation: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Yanchang
authors.initials: Y
authors.lastname: Wang
authors.forename: Takahiro
authors.initials: T
authors.lastname: Shirogane
authors.forename: Dou
authors.initials: D
authors.lastname: Liu
authors.forename: J Wade
authors.initials: JW
authors.lastname: Harper
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Elledge
mesh_terms: D001665:Binding Sites / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D005057:Eukaryotic Cells / Q000166:cytology / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018816:Genes, cdc / Q000502:physiology*; D008938:Mitosis / Q000235:genetics; D015394:Molecular Structure; D011485:Protein Binding / Q000235:genetics; D016601:RNA-Binding Proteins; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics*
chemical_list: C473476:Amn1 protein, S cerevisiae; C082279:CDC15 protein; C513239:CEF1 protein, S cerevisiae; D018797:Cell Cycle Proteins; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D019204:GTP-Binding Proteins