title: Synthesis and enzymatic degradation of epichlorohydrin cross-linked pectins.
abstract: The water solubility of pectin was successfully decreased by cross-linking with increasing amounts of epichlorohydrin in the reaction media. The initial molar ratios of epichlorohydrin/ galacturonic acid monomer in the reaction mixtures were 0, 0.37, 0.56, 0.74, 1.00, 1.47, and 2.44. The resulting epichlorohydrin cross-linked pectins were thus referred to as C-LP0, C-LP37, C-LP56, C-LP75, C-LP100, C-LP150, and C-LP250, respectively. Methoxylation degrees ranged from 60.5 +/- 0.9% to 68.0 +/- 0.6%, and the effective cross-linking degrees, determined by quantification of the hydroxyl anions consumed during the reaction, were 0, 17.8, 26.0, 38.3, 46.5, 53.5, and 58.7%. respectively. After incubating the different cross-linked pectins (0.5% w/v) in 25 mL of 0.05 M acetate-phosphate buffer (pH 4.5), containing 50 microL of Pectinex Ultra SP-L (pectinolytic enzymes), between 60 and 80% of the pectin osidic bounds were broken in less than 1 hr. Moreover, increasing the cross-linking degree only resulted in a weak slowing on the enzymatic degradation velocity.
authors.affiliation: Laboratory of Pharmaceutics and Biopharmaceutics, UFR/SDS, University of Ouagadougou, Ouaga, Burkina Faso.
authors.forename: Rasmané
authors.initials: R
authors.lastname: Semdé
authors.forename: André J
authors.initials: AJ
authors.lastname: Moës
authors.forename: Michel J
authors.initials: MJ
authors.lastname: Devleeschouwer
authors.forename: Karim
authors.initials: K
authors.lastname: Amighi
mesh_terms: D002240:Carbohydrate Sequence; D002626:Chemistry, Pharmaceutical; D003106:Colon; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004339:Drug Compounding; D016503:Drug Delivery Systems; D004355:Drug Stability; D004798:Enzymes / Q000737:chemistry*; D004811:Epichlorohydrin / Q000737:chemistry*; D008969:Molecular Sequence Data; D010368:Pectins / Q000138:chemical synthesis / Q000737:chemistry*; D012995:Solubility; D013997:Time Factors
chemical_list: D003432:Cross-Linking Reagents; D004798:Enzymes; D004811:Epichlorohydrin; D010368:Pectins; C003181:polygalacturonic acid