Identification of genes required for immortalization in human papillomavirus-infected human oral keratinocytes.
The early stages of head and neck cancer are presumed to require a senes of genetic alterations that are not represented by a distinct clinical phenotype. Therefore, genes with altered expression in the preneoplasia may be useful for the early detection of this highly recurrent cancer. In this study, we immortalized normal human oral keratinocytes (NHOK) by retroviral-mediated infection of HPV 16 transforming oncogenes, E6 and E7 (HOK16E6E7). Using the Affymetrix gene chip (U95Av2), we identified 177 known genes and EST that were overexpressed at least 3-fold or above in the immortalized cells, while 133 were down-regulated compared to NHOK. Northern blot analysis showed elevated levels of p55CDC in the immortalized cells, while NHOK showed high basal expression of small proline rich protein (SPRR2). The altered expression of these genes maybe associated with cellular proliferation or differentiation and the early stages of oral carcinogenesis.
Nishitani
Junko
J
Department of Otolaryngology, Charles R. Drew University of Medicine & Science, Los Angeles, CA 90059, USA.
Chen
Zugen
Z
Qin
Min
M
Liu
Feng
F
Chen
Hongying
H
Nelson
Stanley F
SF
Liu
Xuan
X
D015152:Blotting, Northern; D015815:Cell Adhesion Molecules / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004261:DNA Replication / Q000235:genetics; D004798:Enzymes / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006133:Growth Substances / Q000235:genetics; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000821:virology; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D009061:Mouth Mucosa / Q000166:cytology; D009857:Oncogenes; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000235:genetics*; D014412:Tumor Virus Infections / Q000235:genetics*
D015815:Cell Adhesion Molecules; D018797:Cell Cycle Proteins; D004798:Enzymes; D006133:Growth Substances