Relation between reticulocyte count and characteristics of erythrocyte 5'-nucleotidase in dogs, cats, cattle and humans.
To examine substrate specificity and susceptibility to lead, erythrocyte 5'-nucleotidase was measured in dogs, cats, cattle and humans, and its relationship to the reticulocyte count in these species was determined. The reticulocyte count in dogs was similar to that in humans, but the count in cats was higher than that in humans. Reticulocytes were not observed in cattle. The activities of canine erythrocyte 5'-nucleotidase measured using cytidine and uridine 5'-monophosphates, which are preferentially catalyzed by one of the human pyrimidine 5'-nucleotidase isozymes (P5N-I), were similar to those of the human enzyme. The canine enzyme preferentially catalyzed thymidine 3'-monophosphate, which is catalyzed only by human P5N-II, more strongly than the human enzyme. This suggests that canine erythrocytes have two isozymes similar to human P5N-I and P5N-II, and a higher P5N-II-like activity than human erythrocytes. Feline erythrocytes had the highest level of P5N-I-like activity among the species examined, and the bovine enzymic activities including those of P5N-I and II were the lowest among these species. According to these observations, the reticulocyte count was approximately proportional to the P5N-I-like activity in these species. Therefore, the P5N-I-like activity may be involved in the morphological maturation of mammalian erythrocytes. The canine and feline erythrocytes had markedly high activity and preferentially catalyzed purine 5'-monophosphate suggesting the presence of a purine-specific 5'-nucleotidase as in human erythrocytes. In addition, the canine and feline P5N-I-like activity showed less susceptibility to lead than the human P5N-I. This may be a reason why there are few case reports of lead-induced anemia in dogs and cats.
Hossain
Mohammad Alamgir
MA
Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Yamato
Osamu
O
Yamasaki
Masahiro
M
Otsuka
Yayoi
Y
Maede
Yoshimitsu
Y
D015720:5'-Nucleotidase / Q000378:metabolism*; D000818:Animals; D002415:Cats; D002417:Cattle; D002478:Cells, Cultured; D004285:Dogs; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007854:Lead / Q000494:pharmacology; D017701:Reticulocyte Count; D012156:Reticulocytes / Q000166:cytology*; D013045:Species Specificity; D013379:Substrate Specificity
D007527:Isoenzymes; D007854:Lead; D015720:5'-Nucleotidase