title: Acridine mutagenesis of zebrafish (Danio rerio).
abstract: Mutagenesis screening, in which heritable traits are isolated following damage to the genome, is a powerful approach for investigating gene function. Among vertebrate model organisms, the zebrafish (Danio rerio) is ideally suited to mutagenesis screens. The success of large-scale screens is dependent on the way in which changes are identified. The type of damage induced is also pivotal. Single base coding region deletions and insertions are suited to abolition of gene function whilst inducing a small physical alteration to the genome. Such mutations are not commonly found following mutagenesis schemes reported to date. Here, we show that an acridine mutagen, ICR191, which in other model organisms frequently induces single base deletions and insertions, is mutagenic in zebrafish. ICR191 induces hallmark phenotypes associated with genetic damage in treated embryos. Alterations are heritable. Offspring of mutagenised fish had mutations in a marker gene and were found to produce offspring with abnormal development. Using an adaptation of a molecular mutation detection method, fluorescent arbitrary primed PCR, we identified an induced alteration directly. The estimated frequency of induced mutations was sufficiently high to make it feasible to employ this approach for mutagenesis screening.
authors.affiliation: MRC Centre for Developmental Neurobiology, 4th Floor South, New Hunt's House, King's College London, Guy's Campus, London SE1 1UL, UK. richard.hampson@kcl.ac.uk
authors.forename: Richard
authors.initials: R
authors.lastname: Hampson
authors.forename: Simon M
authors.initials: SM
authors.lastname: Hughes
mesh_terms: D000585:Aminacrine / Q000031:analogs & derivatives* / Q000633:toxicity*; D000818:Animals; D003433:Crosses, Genetic; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects; D005393:Fish Diseases / Q000139:chemically induced / Q000235:genetics; D018095:Germ-Line Mutation; D016296:Mutagenesis; D009153:Mutagens / Q000633:toxicity*; D009588:Nitrogen Mustard Compounds / Q000633:toxicity*; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*
chemical_list: D009153:Mutagens; D009588:Nitrogen Mustard Compounds; D000585:Aminacrine; C004294:acridine half-mustard