title: A new approach to the statistical treatment of 2D-maps in proteomics using fuzzy logic.
abstract: A new approach to the statistical treatment of 2D-maps has been developed. This method is based on the use of fuzzy logic and allows to take into consideration the typical low reproducibility of 2D-maps. In this approach the signal corresponding to the presence of proteins on the 2D-maps is substituted with probability functions, centred on the signal itself. The standard deviation of the bidimensional gaussian probability function employed to blur the signal allows to assign different uncertainties to the two electrophoretic dimensions. The effect of changing the standard deviation and the digitalisation resolution are investigated.
authors.affiliation: Dipartimento di Scienze e Tecnologie Avanzate-Università del Piemonte Orientale-Spalto Marengo 33-15100 Alessandria, Italy. marengoe@tin.it
authors.forename: Emilio
authors.initials: E
authors.lastname: Marengo
authors.forename: Elisa
authors.initials: E
authors.lastname: Robotti
authors.forename: Valentina
authors.initials: V
authors.lastname: Gianotti
authors.forename: Pier Giorgio
authors.initials: PG
authors.lastname: Righetti
mesh_terms: D004586:Electrophoresis; D017143:Fuzzy Logic; D008962:Models, Theoretical; D040901:Proteomics