Effect of meliacine, a plant derived antiviral, on tumor necrosis factor alpha.
The effect of meliacine (MAS) and two fractions MAB 1 and MAB 2 obtained from it on the in vitro production of TNF-alpha of murine macrophages induced by bacterial lipopolysaccharide (LPS) (from Escherichia coli) was tested. Simultaneous administration of the above fractions (ranging from 14 to 56 microg/ml) and LPS (10 microg/ml) to a macrophage culture significantly increased the amount of TNF-alpha released at 24 h of induction in a dose-dependent manner. Meliacine alone, at a concentration of 56 microg/ml, is a weak inducer of TNF-alpha production.
Petrera
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Department of Biochemistry, School of Science, University of Buenos Aires, Buenos Aires, Argentina.
Coto
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CE
D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli; D005260:Female; D008070:Lipopolysaccharides; D017737:Macrophages, Peritoneal / Q000187:drug effects; D031586:Melia azedarach; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D018515:Plant Leaves; D010940:Plant Proteins; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects*
D000998:Antiviral Agents; D008070:Lipopolysaccharides; D010455:Peptides; D010936:Plant Extracts; D010940:Plant Proteins; D014409:Tumor Necrosis Factor-alpha; C058031:meliacin