Novosphingobium tardaugens sp. nov., an oestradiol-degrading bacterium isolated from activated sludge of a sewage treatment plant in Tokyo.
An oestradiol-degrading bacterium isolated at a sewage treatment plant in Tokyo was studied phenotypically, genotypically and phylogenetically. Analysis of its 16S rDNA sequence, DNA base composition, whole-cell fatty acid profile and isoprenoid quinone composition, as well as the presence of sphingoglycolipid, revealed that the isolate is a member of the genus Novosphingobium. However, the sequence similarity of its 16S rDNA to those of known Novosphingobium species was no higher than 97%, implying that the isolate is distinctive. The results of DNA-DNA hybridization experiments and physiological characterization also indicated that the isolate represents a novel Novosphingobium species, for which the name Novosphingobium tardaugens sp. nov. is proposed; strain ARI-1T (=JCM 11434T =ATCC BAA-531T =IFO 16725T) is the type strain.
Fujii
Katsuhiko
K
Department of Applied Chemistry, Muroran Institute of Technology, Mizumoto, Muroran 050-8585, Japan.
Satomi
Masataka
M
Food Processing Division, National Research Institute of Fisheries Science, Fukuura, Kanazawa, Yokohama 236-8648, Japan.
Morita
Naoki
N
Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology, Toyohira, Sapporo 062-8517, Japan.
Motomura
Taizo
T
Muroran Marine Station, Field Science Centre for Northern Biosphere, Hokkaido University, Bokoi-minamimachi, Muroran 051-0003, Japan.
Tanaka
Tadaharu
T
Technical Research Laboratory, Kurabo Industries, Shimokida-cho, Neyagawa, Osaka 572-0823, Japan.
Kikuchi
Shintaro
S
Department of Applied Chemistry, Muroran Institute of Technology, Mizumoto, Muroran 050-8585, Japan.
D020561:Alphaproteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001482:Base Composition; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004958:Estradiol / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D014041:Tokyo
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D008055:Lipids; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D004958:Estradiol