Infection of a chimeric simian and human immunodeficiency virus with CCR5-specific HIV-1 envelope to Rhesus macaques.
Human immunodeficiency virus (HIV) infects lymphocytes and macrophages via CD4 and chemokine receptors. In this study, the infectivity of a chimeric simian and human immunodeficiency virus (SHIV) having a CCR5-specific HIV-1 envelope gene was examined. A SHIV strain termed SHIV-JRFL could enter cells via CD4 with a chemokine receptor CCR5, not CXCR4, and the viral replication was suppressed by recombinant human RANTES, one of beta-chemokines. The intravenous inoculation of SHIV-JRFL into two rhesus macaques resulted in a systemic infection, though it was rather weak. During the early infection, the production of RANTES from Con A-stimulated PBMCs of the infected monkeys increased. These results suggested that beta-chemokine has the potential to limit the infectivity of an R5-type SHIV.
Enose
Yoshimi
Y
Institute for Virus Research, Kyoto University.
Miyake
Ariko
A
Ido
Eiji
E
Hayami
Masanori
M
D000818:Animals; D018946:Chemokine CCL5 / Q000032:analysis; D004274:DNA, Recombinant / Q000235:genetics*; D004195:Disease Models, Animal; D006678:HIV / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D008253:Macaca mulatta / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis; D019713:Receptors, CCR5 / Q000378:metabolism*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000378:metabolism*; D019562:Viral Load; D014779:Virus Replication
D018946:Chemokine CCL5; D004274:DNA, Recombinant; D012367:RNA, Viral; D019713:Receptors, CCR5; D014759:Viral Envelope Proteins