[Generation of high affinity humanized single-chain antibody against PreS1 of hepatitis B virus from immune phage displayed antibody library].
OBJECTIVE
Screening and characterizing high affinity completely humanized single-chain antibody (ScFv) against PreS1 of hepatitis B virus.


METHODS
A combinatorial library of phage-displayed human ScFv, genes of which were derived from peripheral blood lymphocytes immunized by PreS1 of Hepatitis B Virus in vitro, was constructed. The library contained 7 10(8) clones.


RESULTS
After 3 rounds panning, a high affinity (K=10(7) to 10(8) mol/L) ScFv specific to PreS1 was obtained. The V(H) belonged to human V(H4) family, and V(1) to V(4) by sequence analysis.


CONCLUSIONS
This study suggests that the method of antigen stimulation in vitro is an expeditious way for the source of human immune antibody. And the ScFv may provide a more satisfactory therapy.
Zhang
Zhi Chao
ZC
Department of Bioengineering, Dalian University of Technology, Dalian 116012, China.
Li
Qi
Q
Hu
Xue Jun
XJ
D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000915:Antibody Affinity; D006510:Hepatitis B Antibodies / Q000096:biosynthesis*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D019151:Peptide Library; D011498:Protein Precursors / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D019151:Peptide Library; D011498:Protein Precursors; C050806:presurface protein 1, hepatitis B surface antigen