Analysis of the presence of Trypanosoma cruzi in the heart tissue of three patients with chronic Chagas' heart disease.
It is still unclear to what extent myocarditis-associated, chronic Chagas' heart disease is due to persisting Trypanosoma cruzi. In the present study, we have analyzed tissue samples from the hearts of three patients with this disease. In situ hybridization provided little evidence for the presence of intact T. cruzi even at sites of strong inflammation. Nevertheless, micromanipulation techniques detected remnants of both T. cruzi kinetoplast DNA and nuclear DNA. Trypanosoma cruzi DNA was also detected in single macrophages dissected directly from frozen heart tissue sections. Thus, this analysis demonstrates that T. cruzi kinetoplast DNA and nuclear DNA are widely dispersed in the heart tissue, although in low amounts. Since we rarely detected intact T. cruzi parasites during the chronic phase of Chagas' heart disease, we can exclude heart tissue as a major parasite reservoir.
Elias
Fernando E
FE
Deutsches Rheuma ForschungsZentrum, Berlin, Germany. f.elias@gmx.net
Vigliano
Carlos A
CA
Laguens
Rubén P
RP
Levin
Mariano J
MJ
Berek
Claudia
C
D000818:Animals; D001483:Base Sequence; D002598:Chagas Cardiomyopathy / Q000469:parasitology*; D002908:Chronic Disease; D017931:DNA Primers; D016054:DNA, Protozoan / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D008969:Molecular Sequence Data; D009205:Myocarditis / Q000469:parasitology*; D009206:Myocardium / Q000473:pathology; D016133:Polymerase Chain Reaction; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000302:isolation & purification
D017931:DNA Primers; D016054:DNA, Protozoan