Flavonoid effects on normal and leukemic cells.
Quercetin and flavopiridol, both flavonoids which influence oxidative milieu, proliferation, and apoptosis of various cell types, were examined for their effects on acute myelogenous leukemic cells and normal progenitors. Both quercetin and flavopiridol inhibited the growth and viability of various acute myelogenous leukemia (AML) cell lines and AML blasts isolated afresh from patients with AML of various subtypes. The effects on inhibition of proliferation and decreased viability were also significant in normal CD34+ cells isolated from normal marrow donors. In certain AML cases, the effects of flavopiridol appeared to be mediated through activation of caspase 3, offering one possible mechanism for the apoptosis evident after exposure to flavopiridol as measured by annexin V expression. These flavonoid compounds might find use in various therapeutic settings in AML.
Liesveld
Jane L
JL
Department of Medicine, James P Wilmot Cancer Center, University of Rochester Medical Center, Rochester, NY, USA. jane_liesveld@urmc.rochester.edu
Abboud
Camille N
CN
Lu
Chaohui
C
McNair
Curtiss
C
Menon
Aarti
A
Smith
Alan
A
Rosell
Karen
K
Rapoport
Aaron P
AP
D000328:Adult; D000368:Aged; D017304:Annexin A5 / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D016022:Case-Control Studies; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003114:Colony-Forming Units Assay; D004789:Enzyme Activation; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010880:Piperidines / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D017304:Annexin A5; D018952:Antigens, CD34; D005419:Flavonoids; D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3; D010880:Piperidines; C077990:alvocidib; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D011794:Quercetin; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases