High-throughput one-bead-one-compound approach to peptide-encoded combinatorial libraries: MALDI-MS analysis of single TentaGel beads.
The identification of pharmacologically promising compounds (lead compounds) from combinatorial libraries is frequently limited by the throughput of the analytical technique employed. Fourier transform mass spectrometry (FTMS) offers high sensitivity, mass accuracy (m/Deltam > 500 000), and sequencing capabilities. A rapid and efficient method for high-throughput analysis of single beads from peptide-encoded combinatorial libraries with matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is presented. Encoding peptides on single beads are identified and structurally characterized by MALDI time-of-flight (TOF) and ultrahigh-resolution MALDI Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. A strategy of on-probe sample preparation is developed to minimize handling of the beads.
Franz
Andreas H
AH
Department of Chemistry, University of California Davis, One Shields Avenue, Davis, California 95616, USA.
Liu
Ruiwu
R
Song
Aimin
A
Lam
Kit S
KS
Lebrilla
Carlito B
CB
D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D017357:Cyclotrons; D008863:Microspheres; D019151:Peptide Library; D010455:Peptides / Q000138:chemical synthesis*; D010782:Photolysis; D000644:Quaternary Ammonium Compounds; D012116:Resins, Plant; D012997:Solvents; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D019151:Peptide Library; D010455:Peptides; D000644:Quaternary Ammonium Compounds; D012116:Resins, Plant; D012997:Solvents