Substrate specificity of NovM: implications for novobiocin biosynthesis and glycorandomization.
[reaction: see text] In an effort to expand the scope of natural product in vitro glycorandomization (IVG), the substrate specificity of NovM was investigated. A test of four aglycon analogues and over 40 nucleotide sugars revealed NovM has a surprisingly stringent substrate specificity and provided only three new "unnatural" natural products. On the basis of the determined substrate specificity, an alternative to the sugar nucleotide biosynthetic dogma and a cautionary note for the general applicability of IVG are introduced.
Albermann
Christoph
C
Laboratory for Biosynthetic Chemistry, Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison, Wisconsin 53705, USA.
Soriano
Aileen
A
Jiang
Jiqing
J
Vollmer
Heidi
H
Biggins
John B
JB
Barton
William A
WA
Lesniak
Jacob
J
Nikolov
Dimitar B
DB
Thorson
Jon S
JS
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D006027:Glycosides / Q000737:chemistry; D009675:Novobiocin / Q000096:biosynthesis*; D013302:Streptomyces; D013379:Substrate Specificity
D000900:Anti-Bacterial Agents; D006027:Glycosides; D009675:Novobiocin