title: In vitro aging of macaque adherent cells: similar pattern of cellular aging between human and macaque.
abstract: To explore new models for human cellular aging as well as to evaluate aging of the macaques, profiles of cellular aging in macaques were studied. Adherent cells were obtained from five Japanese macaques (Macaca fuscata), 14 long-tailed macaques (Macaca fascicularis), two bonnet monkeys (Macaca radiata) and a rhesus monkey (Macaca mulatta). A total of 35 cultures were performed and cell morphology, doubling time, telomere length and telomerase activity were studied. They were classified into three groups; group I: cell strains with a definite replicative life-span (-41 PDLs) (presence of M1), group II: cell strains with a limited extension of replicative life-span (79-106 PDLs) with p53 mutation(s) (presence of M2), and group III: a cell strain with an indefinite replicative life-span (>150 PDLs) with characteristics of transformation. Except for the last group, telomerase activity was not observed. Macaque cells demonstrated three chronological patterns comprising both human and rodent patterns, however, presence of the two limits of proliferation in vitro grants macaque cells to be more appropriate than rodents in both studying human aging and oncogenesis.
authors.affiliation: Department of Biological Sciences, Unit of Human Biology and Genetics, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Juri
authors.initials: J
authors.lastname: Suzuki
authors.forename: Keiji
authors.initials: K
authors.lastname: Terao
authors.forename: Takafumi
authors.initials: T
authors.lastname: Ishida
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000166:cytology; D008168:Lung / Q000166:cytology; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008255:Macaca radiata; D008297:Male; D010641:Phenotype; D012867:Skin / Q000166:cytology*; D013045:Species Specificity; D019098:Telomerase / Q000378:metabolism; D016615:Telomere / Q000378:metabolism
chemical_list: D019098:Telomerase