Kinetic analysis of pulmonary neutrophil retention in vivo using the multiple-indicator-dilution technique.
Multiple-indicator-dilution experiments were performed in the lungs of 13 anesthetized dogs by simultaneous bolus injection of 111In-labeled neutrophils, 51Cr-labeled red blood cells, and Evans blue-labeled albumin. Concomitant counts of unlabeled neutrophils were similar in pulmonary artery and aortic blood samples, demonstrating a dynamic balance across the lungs in the physiological state. Outflow profiles of labeled neutrophils were analyzed on the basis of a recirculatory pharmacokinetic model of labeled albumin. The outflow profiles of the recovered neutrophils were composed of a throughput component of circulating neutrophils and a component of reversibly marginated neutrophils. They were interpreted by a model incorporating neutrophil margination (transfer coefficient = 0.195 +/- 0.081 s-1), rapid demargination (0.054 +/- 0.027 s-1), and transfer to a slow marginated pool (0.023 +/- 0.018 s-1). It will be interesting to apply the analysis in future studies aimed at determining whether it could be a useful research tool to investigate the interactions between the pulmonary endothelium and neutrophils in physiological and diseased states.
Schwab
Andreas J
AJ
McGill University Medical Clinic, MontrealGeneral Hospital,Quebec, Canada H3G, 1A4.
Salamand
Agnes
A
Merhi
Yahye
Y
Simard
Andre
A
Dupuis
Jocelyn
J
D000418:Albumins / Q000378:metabolism; D000465:Algorithms; D000818:Animals; D004285:Dogs; D004395:Dye Dilution Technique; D006439:Hemodynamics / Q000502:physiology; D007205:Indium Radioisotopes; D007700:Kinetics; D007958:Leukocyte Count; D008168:Lung / Q000166:cytology*; D008954:Models, Biological; D015233:Models, Statistical; D009504:Neutrophils / Q000502:physiology*; D011652:Pulmonary Circulation / Q000502:physiology; D011659:Pulmonary Gas Exchange / Q000502:physiology
D000418:Albumins; D007205:Indium Radioisotopes