The solution structure of the Ga(III)-bleomycin A2 complex resolved by NMR and molecular modeling; interaction with d(CCAGGCCTGG).
The solution structure of the Ga(III)-bleomycin A2 complex (GaBLM) has been determined using 2D NMR methods in combination with molecular dynamics calculations. Complete assignment of the amide and amine protons, observation of 80 NOEs and measurement of 15 (3)JH(-H) coupling constants provided us with a well-defined structure using a restrained simulated annealing protocol. On the basis of distance and dihedral angle constraints agreement, along with potential energy considerations, the favored model is a five-coordinate complex with the primary amine of beta-aminoalanine holding the axial position of a distorted tetragonal pyramid. The disaccharide moiety of GaBLM is not a ligand, sharing the same side of the equatorial plane with the axial amine ligand. Titration of the self-complementary oligonucleotide d(CCAGGCCTGG) with GaBLM results in the formation of only one 1:1 complex in slow exchange on the NMR time scale. Our data indicate that the bithiazole moiety intercalates between the C6*G15 and C7*G14 base pairs, in a similar mode to that reported by earlier studies. Structural implications and comparisons to other metallo-bleomycins are discussed.
Papakyriakou
Athanasios
A
Institute of Physical Chemistry, NCSR "Demokritos", 153-10 Ag. Paraskevi Attikis, Athens, Greece.
Mouzopoulou
Barbara
B
Institute of Physical Chemistry, NCSR "Demokritos", 153-10 Ag. Paraskevi Attikis, Athens, Greece.
Katsaros
Nikos
N
Institute of Physical Chemistry, NCSR "Demokritos", 153-10 Ag. Paraskevi Attikis, Athens, Greece. katsaros@chem.demokritos.gr.
D001761:Bleomycin / Q000737:chemistry*; D005708:Gallium / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008958:Models, Molecular; D009841:Oligonucleotides / Q000737:chemistry*; D011485:Protein Binding; D012996:Solutions
D009841:Oligonucleotides; D012996:Solutions; D001761:Bleomycin; D005708:Gallium