Close identity of a pressure-stabilized intermediate with a kinetic intermediate in protein folding.
Atomic detailed structural study of a transiently existing folding intermediate is severely limited because of its short life. In ubiquitin, we found that a pressure-stabilized equilibrium conformer shares a common structural feature with the proline-trapped kinetic intermediate found in a pulse-labeling (1)H(2)H exchange NMR study [Briggs, M. S. & Roder, H. (1992) Proc. Natl. Acad. Sci. USA 89, 2017-2021]. The conformer is locally unfolded in the entire segment from residues 33 to 42 and in C-terminal residues 70-76. The close structural identity of an equilibrium intermediate stabilized under pressure with a transiently observed folding intermediate is likely to be general in terms of a folding funnel common to both experiments.
Kitahara
Ryo
R
Department of Molecular Science, Graduate School of Science and Technology, Kobe University, Rokkodai-cho, Kobe 657-8501, Japan.
Akasaka
Kazuyuki
K
D003903:Deuterium; D004355:Drug Stability; D006859:Hydrogen; D066298:In Vitro Techniques; D007700:Kinetics; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D011312:Pressure; D011487:Protein Conformation; D017510:Protein Folding; D013816:Thermodynamics; D025801:Ubiquitin / Q000737:chemistry*
D025801:Ubiquitin; D006859:Hydrogen; D003903:Deuterium