title: The Musca domestica larval hexamerin is composed of multiple, similar polypeptides.
abstract: The Musca domestica larval hexamerin (MdHex-L) is a hexameric glycoprotein with an apparent native molecular weight of 500 kDa. Seven different cDNAs that encode MdHex-L subunits were cloned and sequenced. Furthermore, amino acid sequences of isolated subunits were determined by the Edman degradation method and compared to the conceptual translation products derived from the cloned cDNAs. The obtained data indicate the existence of multiple forms of MdHex-L subunits and that these multiple forms may be grouped into three categories according to their percentages of nucleotide sequence identity.
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP 05508-900, Brazil.
authors.forename: C K
authors.initials: CK
authors.lastname: Moreira
authors.forename: M de L
authors.initials: Mde L
authors.lastname: Capurro
authors.forename: E
authors.initials: E
authors.lastname: Calvo
authors.forename: P I
authors.initials: PI
authors.lastname: Silva
authors.forename: A A
authors.initials: AA
authors.lastname: James
authors.forename: A G
authors.initials: AG
authors.lastname: deBianchi
authors.forename: O
authors.initials: O
authors.lastname: Marinotti
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D006458:Hemolymph; D006793:Houseflies / Q000254:growth & development*; D019476:Insect Proteins / Q000737:chemistry* / Q000235:genetics; D007814:Larva; D008969:Molecular Sequence Data; D010802:Phylogeny; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D019476:Insect Proteins; D011994:Recombinant Proteins; C041137:storage proteins, Insecta