Direct observation of structure transition of donor-acceptor labeled peptides temperature dependence of fluorescence quenching kinetics.
From the temperature dependence of fluorescence quenching kinetics, a novel temperature-sensitive structure transition was discovered in a donor-acceptor labeled peptides. Perfect agreement of related abrupt changes in a(1),tau(1) and tau(2) gives strong support for the existence of this structure transition, which suggests it is possible to develop peptides that can be rapidly and reversibly switched between two structure states in response to temperature shift.
Min
Wei
W
Department of Applied Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P.R. China. minw@chem.pku.edu.cn
Sun
Lin
L
D000595:Amino Acid Sequence; D004151:Dipeptides / Q000737:chemistry*; D004579:Electron Transport; D005453:Fluorescence; D007700:Kinetics; D009842:Oligopeptides / Q000737:chemistry*; D038761:Photobleaching; D017433:Protein Structure, Secondary; D013696:Temperature
D004151:Dipeptides; D009842:Oligopeptides