title: Specific conjugation of DNA binding proteins to DNA templates through thiol-disulfide exchange.
abstract: The double-stranded oligodeoxyribonucleotides with single internucleotide disulfide linkages were successfully used for covalent trapping of cysteine containing protein. In particular, an efficient conjugation of DNA methyltransferase SsoII to sequence-specific decoys was demonstrated. The obtained results assume that synthetic oligodeoxyribonucleotides bearing a new trapping site can be used as new tools to study and manipulate biological systems.
authors.affiliation: Chemical Department, M V Lomonosov Moscow State University, Vorob'evi gori, Moscow 119992, Russia.
authors.forename: Valeri G
authors.initials: VG
authors.lastname: Metelev
authors.forename: Elena A
authors.initials: EA
authors.lastname: Kubareva
authors.forename: Olga V
authors.initials: OV
authors.lastname: Vorob'eva
authors.forename: Andrey S
authors.initials: AS
authors.lastname: Romanenkov
authors.forename: Tatiana S
authors.initials: TS
authors.lastname: Oretskaya
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004220:Disulfides / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013698:Templates, Genetic
chemical_list: D004268:DNA-Binding Proteins; D004220:Disulfides; D013438:Sulfhydryl Compounds; D004247:DNA