Induction of glutathione-S-transferase in the Northern quahog Mercenaria mercenaria after exposure to the polychlorinated biphenyl (PCB) mixture Aroclor 1248.
The glutathione-S-transferases (GSTs) from the Northern quahog (Mercenaria mercenaria) were examined after an injection of a polychlorinated biphenyl (PCB) mixture, Aroclor 1248, to a concentration of approximately 50 ppm. Enzymatic analysis indicated a fourfold increase in the GST activity of quahogs injected with PCBs compared with that of the control. An electrophoretic analysis of the GST from the PCB-exposed quahogs showed a 1.5-fold increase in the concentration over that of the control. Purification of the GST on a glutathione affinity column yielded a glutathione binding protein, in addition to the GSTs. However, the amount of the glutathione binding protein in the PCB-injected quahogs was found to decrease by approximately 50% in comparison to the glutathione binding protein in the control quahogs.
Blanchette
Brian N
BN
Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth and The School for Marine Science and Technology, 285 Old Westport Road, North Dartmouth, Massachusetts 02747, USA.
Singh
Bal Ram
BR
D000818:Animals; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D004785:Environmental Pollutants; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000378:metabolism; D007700:Kinetics; D008974:Mollusca / Q000187:drug effects* / Q000201:enzymology; D011078:Polychlorinated Biphenyls
D004785:Environmental Pollutants; D011078:Polychlorinated Biphenyls; D005982:Glutathione Transferase; D005978:Glutathione