title: cDNA encodes Xenopus P2Y(1) nucleotide receptor: expression at the neuromuscular junctions.
abstract: A cDNA encoding P2Y(1) receptor was isolated by cross-hybridization with chicken homolog. The deduced amino acid sequence of P2Y(1) receptor with 361 amino residues is 80-85% identical to human, rodent and avian homologs. When the cDNA was expressed in mammalian cells, the activation of P2Y(1) receptor by adenine nucleotides stimulated the accumulation of inositol phosphate, and adenosine 3',5'-bismonophosphate (A3P5P) or other antagonists blocked its action; these pharmacological properties showed resemblance of P2Y(1) receptor family in higher vertebrate. A transcript encoding P2Y(1) receptor at approximately 3.2 kb was revealed in the brain, spinal cord and muscle of adult, and it is strongly expressed in developing brain, spinal cord and myotomal muscles of the embryos by hybridization. P2Y(1) receptor was shown to be restricted to the neuromuscular junctions and co-localized with AChRs in adult muscle. These results support the notion that ATP and its P2Y(1) receptor subtype are effectors in organizing the post-synaptic apparatus.
authors.affiliation: Department of Biology and Molecular Neuroscience Center, The Hong Kong University of Science and Technology, Hong Kong, China.
authors.forename: Anthony W M
authors.initials: AW
authors.lastname: Cheng
authors.forename: Ling W
authors.initials: LW
authors.lastname: Kong
authors.forename: Edmund K K
authors.initials: EK
authors.lastname: Tung
authors.forename: Nina L
authors.initials: NL
authors.lastname: Siow
authors.forename: Roy C Y
authors.initials: RC
authors.lastname: Choi
authors.forename: Shang Q
authors.initials: SQ
authors.lastname: Zhu
authors.forename: Benjamin H
authors.initials: BH
authors.lastname: Peng
authors.forename: Karl W K
authors.initials: KW
authors.lastname: Tsim
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009469:Neuromuscular Junction / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018048:Receptors, Purinergic P2 / Q000235:genetics* / Q000378:metabolism*; D058472:Receptors, Purinergic P2Y1; D013194:Staining and Labeling; D014981:Xenopus / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; C546382:P2RY1 protein, human; D012333:RNA, Messenger; D018048:Receptors, Purinergic P2; D058472:Receptors, Purinergic P2Y1