title: Cloning and identification of a novel ubiquitin-like protein, BMSC-UbP, from human bone marrow stromal cells.
abstract: Ubiquitin is one of phylogenetically well-conserved proteins in all eukaryotes. Ubiquitin-dependent modification of protein contributes to fine regulation of cellular biological processes. Using large-scale screening of human bone marrow stromal cell (BMSC) cDNA library, we isolated a full-length cDNA of 1352 bp encoding 380 amino acids with a ubiquitin domain (UBQ), which was designed as bone marrow stromal cell-derived ubiquitin-like protein (BMSC-UbP). In addition to UBQ domain at its N-terminus, BMSC-UbP also possesses a ubiquitin-associated domain at its C-terminus, sharing moderate homology to some ubiquitin-like proteins such as UBIN, Chap1, and ubiquilin. BMSC-UbP localizes at chromosome 15q22.3-q23 as confirmed by blast search in human genome. BMSC-UbP mRNA is widely expressed in human multiple tissues and various tumor cell lines. Moreover, BMSC-UbP mRNA decreased in BMSC stimulated with PMA and increased in HL60 cells stimulated with LPS, suggesting that BMSC-UbP might play roles in regulation of BMSC function or cell differentiation through an evocator- and cell-specific pattern.
authors.affiliation: Institute of Immunology, Second Military Medical University, 800 Xiangyin Road, 200433, Shanghai, People's Republic of China.
authors.forename: Shuxun
authors.initials: S
authors.lastname: Liu
authors.forename: Yizhi
authors.initials: Y
authors.lastname: Yu
authors.forename: Huazhang
authors.initials: H
authors.lastname: An
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Naisong
authors.initials: N
authors.lastname: Lin
authors.forename: Wenya
authors.initials: W
authors.lastname: Wang
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Wan
authors.forename: Xuetao
authors.initials: X
authors.lastname: Cao
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology*; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D017154:Stromal Cells / Q000737:chemistry*; D014018:Tissue Distribution; D014452:Ubiquitins / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; C479964:UBL7 protein, human; D014452:Ubiquitins