Pseudoalteromonas agarivorans sp. nov., a novel marine agarolytic bacterium.
The phenotypic, genomic and phylogenetic characteristics of four aerobic, Gram-negative, non-fermentative, motile, non-pigmented, agarolytic Pseudoalteromonas-like bacteria, isolated from marine environments, have been investigated. These bacteria share DNA-DNA similarities above 86%. Comparative 16S rDNA sequence analysis of strain KMM 255T revealed its membership of the genus Pseudoalteromonas; it shares 99.9% sequence similarity with Pseudoalteromonas distincta, Pseudoalteromonas elyakovii, Pseudoalteromonas atlantica and Pseudoalteromonas espejiana. DNA-DNA reassociation levels obtained for strain KMM 255T and type strains of these four species and other Pseudoalteromonas species were below 45%. The marine isolates differed from known species of the genus by the fact that the cells are motile by means of a single flagellum or two to four polar unsheathed flagella and by an inability to utilize most organic compounds. On the basis of phenotypic, DNA-DNA hybridization and phylogenetic data, it is concluded that the isolates represent a novel species within the genus Pseudoalteromonas, for which the name Pseudoalteromonas agarivorans sp. nov. is proposed. The type strain is strain KMM 255T (= DSM 14585T).
Romanenko
Lyudmila A
LA
Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, 690022 Vladivostok, Prospekt 100 Let Vladivostoku, 159, Russia.
Zhukova
Natalia V
NV
Institute of Marine Biology, Far-Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, Russia.
Rohde
Manfred
M
GBF - Gesellschaft für Biotechnologische Forschung GmbH, D-38124 Braunschweig, Germany.
Lysenko
Anatoly M
AM
Institute of Microbiology, Russian Academy of Sciences, 117811 Moscow, Russia.
Mikhailov
Valery V
VV
Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, 690022 Vladivostok, Prospekt 100 Let Vladivostoku, 159, Russia.
Stackebrandt
Erko
E
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010641:Phenotype; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D031161:Pseudoalteromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S