Development and characterization of simulant pancreatic islets.
Insulin is stored in pancreatic islets as a zinc-insulin complex, and stimulating the islets results in the release of insulin and zinc. Simulant pancreatic islet beads have been developed using agarose beads (50-250 micro m diameter) derivatized with iminodiacetic acid that have been loaded with zinc. A qualitative comparison of the simulant beads with pancreatic islets has been made by staining with dithizone and a zinc-binding fluorescent dye, TSQ. The binding capacity of simulant beads was determined to be 34 micro mol Zn(2+)/g of dried beads using anodic stripping voltammetry. Hydrochloric acid was used to release zinc from beads to mimic the secretion of insulin from pancreatic islets and a release profile was established. The simulant beads can be used to optimize the islet isolation process and reduce the use of real islets in method development.
Maghasi
Anne T
AT
Department of Chemistry, University of Cincinnati, P.O. Box 210172, Cincinnati, OH 45221-0172, USA.
Schlueter
Kevin T
KT
Halsall
H Brian
HB
Heineman
William R
WR
Rodriguez Rilo
Horacio L
HL
D000818:Animals; D004285:Dogs; D004563:Electrochemistry; D006851:Hydrochloric Acid / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008863:Microspheres; D051381:Rats; D012685:Sepharose / Q000378:metabolism* / Q000494:pharmacology; D013194:Staining and Labeling; D015032:Zinc / Q000378:metabolism
D007328:Insulin; D012685:Sepharose; D015032:Zinc; D006851:Hydrochloric Acid