ADAM 10: an active metalloprotease expressed during avian epithelial morphogenesis.
The ADAMs are a family of proteins containing multiple functional domains. We have cloned the avian orthologue of ADAM 10 and demonstrate that it has metalloprotease activity. Chick ADAM 10 is expressed in the developing dermatome and myotome of the somite, epidermis, gut endoderm, the epithelial tissues of the kidney, liver, and heart, and in neural crest cells. The expression patterns and protein distribution of ADAM 10 suggest it may play a significant role in the morphogenesis of several epithelial tissues. When a dominant-negative metalloprotease-mutant form of ADAM 10 is expressed in the ectoderm or ADAM 10 expression is knocked down with morpholinos, morphogenesis and tissue specification are altered.
Hall
Ronelle J
RJ
Section of Molecular and Cellular Biology, University of California-Davis, One Shields Avenue, Davis, CA 95616, USA. rjyoung@ucdavis.edu
Erickson
Carol A
CA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004848:Epithelium / Q000196:embryology; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting; D006321:Heart / Q000196:embryology; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics; D009432:Neural Crest / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D019170:Somites / Q000378:metabolism
D018076:DNA, Complementary; D008565:Membrane Proteins; D012333:RNA, Messenger; D008666:Metalloendopeptidases