title: Distribution of ehrlichiae in tissues as determined by in-situ hybridization.
abstract: Specific identification of ehrlichiae in the tissues and determination of their distribution is difficult. In this study, an in-situ hybridization method was developed to detect ehrlichial 16S rRNA in tissue specimens from mice experimentally infected with the HF strain. This strain is closely related to Ehrlichia chaffeensis, the agent of human monocytic ehrlichiosis. HF strain-specific 16S rRNA was detected in endothelial cells and monocyte-macrophages in the liver, lungs, bone marrow, spleen, lymph nodes, and large and small intestinal tissues. The results suggest that the in-situ hybridization method with a digoxigenin-labelled RNA probe specific to ehrlichial 16S rRNA will be useful for post-mortem diagnosis and for the histopathological investigation of ehrlichial infection.
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, 069-8501, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Okada
authors.forename: H
authors.initials: H
authors.lastname: Usuda
authors.forename: T
authors.initials: T
authors.lastname: Tajima
authors.forename: M
authors.initials: M
authors.lastname: Kawahara
authors.forename: T
authors.initials: T
authors.lastname: Yoshino
authors.forename: Y
authors.initials: Y
authors.lastname: Rikihisa
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D018469:Ehrlichia chaffeensis / Q000235:genetics / Q000302:isolation & purification*; D016873:Ehrlichiosis / Q000382:microbiology* / Q000473:pathology; D004730:Endothelium, Vascular / Q000382:microbiology / Q000473:pathology; D017403:In Situ Hybridization / Q000379:methods*; D008264:Macrophages / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D009000:Monocytes / Q000382:microbiology / Q000473:pathology; D012313:RNA / Q000737:chemistry; D015347:RNA Probes / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D015347:RNA Probes; C034477:RNA primers; D012336:RNA, Ribosomal, 16S; D012313:RNA