Gene discovery in Eimeria tenella by immunoscreening cDNA expression libraries of sporozoites and schizonts with chicken intestinal antibodies.
Specific antibodies were produced ex vivo from intestinal culture of Eimeria tenella infected chickens. The specificity of these intestinal antibodies was tested against different parasite stages. These antibodies were used to immunoscreen first generation schizont and sporozoite cDNA libraries permitting the identification of new E. tenella antigens. We obtained a total of 119 cDNA clones which were subjected to sequence analysis. The sequences coding for the proteins inducing local immune responses were compared with nucleotide or protein databases and with expressed sequence tags (ESTs) databases. We identified new Eimeria genes coding for heat shock proteins, a ribosomal protein, a pyruvate kinase and a pyridoxine kinase. Specific features of other sequences are discussed.
Réfega
Susana
S
Laboratoire de Virologie et Immunologie Moléculaires INRA F 78352, Jouy-en-Josas, France.
Girard-Misguich
Fabienne
F
Bourdieu
Christiane
C
Péry
Pierre
P
Labbé
Marie
M
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000918:Antibody Specificity; D001483:Base Sequence; D002645:Chickens / Q000276:immunology / Q000469:parasitology; D003048:Coccidiosis / Q000276:immunology / Q000469:parasitology; D030541:Databases, Genetic; D016786:Eimeria tenella / Q000235:genetics* / Q000254:growth & development / Q000276:immunology*; D015723:Gene Library; D017125:Genes, Protozoan / Q000235:genetics*; D007422:Intestines / Q000276:immunology; D012680:Sensitivity and Specificity; D034101:Sporozoites / Q000235:genetics* / Q000276:immunology*
D000913:Antibodies, Protozoan