title: Cloning of the pig renal organic anion transporter 1 (pOAT1).
abstract: A pig kidney cDNA library was screened for the porcine ortholog of the multispecific organic anion transporter 1 (pOAT1). Several positive clones were isolated resulting in two alternatively spliced cDNA clones of pOAT1 (pOAT1 and pOAT1A). pOAT1-cDNAs consist of 2126 or 1895 base pairs (EMBL Acc. No. AJ308234 and AJ308235) encoding 547 or 533 amino acid residue proteins with 89, 87, 83 and 81% homology to the human, rabbit, rat, and mouse OAT1, respectively. Heterologous expression of pOAT1 in Xenopus laevis oocytes revealed an apparent K(m) for [3H]PAH of 3.75 +/- 1.6 microM. [3H]PAH uptake mediated by pOAT1 was abolished by 0.5 mM glutarate or 1 mM probenecid. Functional characterization of pOAT1A did not show any affinity for [3H]PAH. In summary, we cloned two alternative splice variants of the pig ortholog of organic anion transporter 1. One splice form (pOAT1) showed typical functional characteristics of organic anion transporter 1, whereas the second form appears not to transport PAH.
authors.affiliation: Zentrum für Physiologie und Pathophysiologie, Abt Vegetative Physiologie, Universität Göttingen, Humboldtallee 23, 37073 Göttingen, Germany. hagos@veg-physiol.med.uni-goettingen.de
authors.forename: Yohannes
authors.initials: Y
authors.lastname: Hagos
authors.forename: Andrew
authors.initials: A
authors.lastname: Bahn
authors.forename: Abdul R
authors.initials: AR
authors.lastname: Asif
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Krick
authors.forename: Mark
authors.initials: M
authors.lastname: Sendler
authors.forename: Gerhard
authors.initials: G
authors.lastname: Burckhardt
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D000838:Anions / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015723:Gene Library; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology; D027362:Organic Anion Transport Protein 1 / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013552:Swine / Q000235:genetics*; D014982:Xenopus laevis; D010130:p-Aminohippuric Acid / Q000493:pharmacokinetics
chemical_list: D000838:Anions; D002352:Carrier Proteins; D018076:DNA, Complementary; D027362:Organic Anion Transport Protein 1; D020033:Protein Isoforms; D010130:p-Aminohippuric Acid