title: [Polymerase chain reaction--application of the technique].
abstract: Our knowledge of genes and genetic diseases has increased tremendously since the polymerase chain reaction technique (PCR) was introduced as a technique in molecular biology. The technique has become a critical tool for basic research, molecular biology, and biotechnology. PCR has been the link between science and molecular biology as a diagnostic tool. High sensitivity within a short time and low labour cost have been the key elements for the success of PCR. Furthermore, combinations with other techniques in molecular biology have opened up for still new perspectives as e.g. fluorescence-based real-time reverse transcription PCR (RT-PCR). In addition to providing a snapshot of the state-of-the-art applications of PCR, a look is made into the new DNA-micro-array era.
authors.affiliation: AH Diagnostics, Runetoften 18, DK-8210 Arhus V.
authors.forename: Niels
authors.initials: N
authors.lastname: Rüdiger
mesh_terms: D000483:Alleles; D014644:Genetic Variation; D006801:Humans; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards / Q000639:trends; D020133:Reverse Transcriptase Polymerase Chain Reaction