Taenia solium cDNA sequence encoding a putative immunodiagnostic antigen for human cysticercosis.
A T. solium metacestode cDNA library was prepared and antibody screened to obtain recombinant antigens, which could be used for the neurocysticercosis diagnosis. The F18 clone was selected and sequenced, and the full length cDNA characterised as well as the genomic structure from the gene. F18 is a single copy gene that spans approximately 6.1 kb and contains five exons and four introns. The F18 cDNA has a 690-nucleotide open reading frame that encodes a putative polypeptide of 229 amino acids with a predicted molecular mass of 26.06 x 10(3) M(r). The F18 recombinant protein was obtained and purified by affinity chromatography using pGEX system (G-F18) and pQE system (H-F18). The purified G-F18 fusion protein showed the best results when it was used in ELISA with sera from neurocysticercosis patients.
Montero
E
E
Instituto de Salud Carlos III, Centro Nacional de Microbiologi;a, Crta. Majadahonda Pozuelo Km 2,2, 28220 Majadahonda, Madrid, Spain.
González
L M
LM
Harrison
L J S
LJ
Parkhouse
R M E
RM
Gárate
T
T
D000595:Amino Acid Sequence; D000947:Antigens, Helminth / Q000097:blood / Q000737:chemistry; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D017931:DNA Primers; D018076:DNA, Complementary; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D020019:Neurocysticercosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D016366:Open Reading Frames; D041201:Taenia solium / Q000235:genetics* / Q000276:immunology
D000947:Antigens, Helminth; D017931:DNA Primers; D018076:DNA, Complementary