title: Cytoskeletal requirements for hepatitis C virus (HCV) RNA synthesis in the HCV replicon cell culture system.
abstract: Hepatitis C virus (HCV) induces microtubule aggregates in infected hepatocytes. To determine if cytoskeletal elements are important for HCV RNA synthesis, we examined the effect of cytoskeleton inhibitors on HCV replicon transcription in Huh7 cells. The data demonstrate that HCV replication complex-mediated RNA synthesis requires microtubule and actin polymerization.
authors.affiliation: Infectious Diseases Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285-0438, USA.
authors.forename: Anne G
authors.initials: AG
authors.lastname: Bost
authors.forename: Daryl
authors.initials: D
authors.lastname: Venable
authors.forename: Lifei
authors.initials: L
authors.lastname: Liu
authors.forename: Beverly A
authors.initials: BA
authors.lastname: Heinz
mesh_terms: D000199:Actins / Q000378:metabolism; D002460:Cell Line; D003599:Cytoskeleton / Q000821:virology; D017930:Genes, Reporter; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008870:Microtubules / Q000821:virology; D020871:RNA Stability; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics*; D012093:Replicon; D014779:Virus Replication
chemical_list: D000199:Actins; D012367:RNA, Viral; D008156:Luciferases