title: PARP-3 localizes preferentially to the daughter centriole and interferes with the G1/S cell cycle progression.
abstract: A novel member of the poly(ADP-ribose) polymerase (PARP) family, hPARP-3, is identified here as a core component of the centrosome. hPARP-3 is preferentially localized to the daughter centriole throughout the cell cycle. The N-terminal domain (54 amino acids) of hPARP-3 is responsible for its centrosomal localization. Full-length hPAPR-3 (540 amino acids, with an apparent mass of 67 kDa) synthesizes ADP-ribose polymers during its automodification. Overexpression of hPARP-3 or its N-terminal domain does not influence centrosomal duplication or amplification but interferes with the G1/S cell cycle progression. PARP-1 also resides for part of the cell cycle in the centrosome and interacts with hPARP-3. The presence of both PARP-1 and PARP-3 at the centrosome may link the DNA damage surveillance network to the mitotic fidelity checkpoint.
authors.affiliation: Unité 9003 du CNRS, Ecole Supérieure de Biotechnologie de Strasbourg, Boulevard Sébastien Brant, 67400 Illkirch, France.
authors.forename: Angélique
authors.initials: A
authors.lastname: Augustin
authors.forename: Catherine
authors.initials: C
authors.lastname: Spenlehauer
authors.forename: Hélène
authors.initials: H
authors.lastname: Dumond
authors.forename: Josiane
authors.initials: J
authors.lastname: Ménissier-De Murcia
authors.forename: Matthieu
authors.initials: M
authors.lastname: Piel
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Schmit
authors.forename: Françoise
authors.initials: F
authors.lastname: Apiou
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Vonesch
authors.forename: Michael
authors.initials: M
authors.lastname: Kock
authors.forename: Michel
authors.initials: M
authors.lastname: Bornens
authors.forename: Gilbert
authors.initials: G
authors.lastname: De Murcia
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D002502:Centrioles / Q000378:metabolism*; D006224:Cricetinae; D016193:G1 Phase / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D051379:Mice; D008969:Molecular Sequence Data; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism*; D016196:S Phase / Q000187:drug effects; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D018797:Cell Cycle Proteins; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C480979:PARP3 protein, human; D011065:Poly(ADP-ribose) Polymerases; D006918:Hydroxyurea