Role of NF-kappaB and AP-1 on Helicobater pylori-induced IL-8 expression in AGS cells.
Oxygen radicals are important regulators in Helicobacter pylori-induced gastric ulceration and carcinogenesis. IL-8 may be regulated by oxidant-sensitive transcription factors, NF-kappaB, and AP-1. The present study aims to investigate whether H. pylori-induced IL-8 expression is regulated by NF-kappaB and AP-1 in gastric epithelial AGS cells and whether this transcriptional regulation of IL-8 is inhibited by N-acetylcysteine (NAC). As a result, H. pylori induced the expression of mRNA and protein for IL-8 via activation of NF-kappaB and AP-1. NF-kappaB activation accompanied by a decrease in I-kappaBalpha and activated AP-1 complex was a c-jun/c-fos heterodimer in H. pylori-infected AGS cells. NAC inhibited H. pylori-induced activation of transcription factors and IL-8 expression in AGS cells. In conclusion, oxygen radicals induce the activation of NF-kappaB and AP-1 and IL-8 expression. Antioxidants such as NAC might be useful anti-inflammatory agents by inhibiting activation of transcription factors and decreasing IL-8 production in H. pylori-induced gastric inflammation.
Chu
Sang Hui
SH
Department of Pharmacology and Institute of Gastroenterology, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
Kim
Hyeyoung
H
Seo
Jeong Yeon
JY
Lim
Joo Weon
JW
Mukaida
Naofumi
N
Kim
Kyung Hwan
KH
D000704:Analysis of Variance; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002478:Cells, Cultured; D004847:Epithelial Cells; D005753:Gastric Mucosa / Q000166:cytology / Q000382:microbiology; D016481:Helicobacter Infections / Q000473:pathology; D016480:Helicobacter pylori; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis / Q000378:metabolism*; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000032:analysis / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011336:Probability; D012333:RNA, Messenger / Q000032:analysis*; D017382:Reactive Oxygen Species; D012680:Sensitivity and Specificity; D013276:Stomach Ulcer / Q000382:microbiology / Q000473:pathology; D018808:Transcription Factor AP-1 / Q000032:analysis / Q000378:metabolism*
D016209:Interleukin-8; D016328:NF-kappa B; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D018808:Transcription Factor AP-1