Pulsatilloside A and anemoside A3 protect PC12 cells from apoptosis induced by sodium cyanide and glucose deprivation.
Using sodium cyanide (NaCN) and glucose deprivation induced cell injury in PC12 as an injury model, we investigated the protective effects of pulsatilloside A and anemoside A 3 on neurons. The results showed that PC12 cells under the NaCN-injury and glucose deprivation would undergo apoptosis. Additions of pulsatilloside A and anemoside A 3, at dosages ranging from 0.1, 1 and 10 microg/ml, protected PC12 cells from apoptosis determined by MTT, LDH release analysis, and flow cytometry measurement.
Gao
Xiang-Dong
XD
Ye
Wen-Cai
WC
Yu
Albert C H
AC
Zhang
Yun
Y
Tan
Ren-Xiang
RX
Li
Ming
M
Hsiao
W L Wendy
WL
D000544:Alzheimer Disease / Q000188:drug therapy; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005947:Glucose; D006801:Humans; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D016716:PC12 Cells / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D031946:Pulsatilla; D051381:Rats; D012503:Saponins; D012966:Sodium Cyanide; D014315:Triterpenes
D018696:Neuroprotective Agents; D010936:Plant Extracts; C473641:Pulsatilloside A; D012503:Saponins; D014315:Triterpenes; C473640:anemoside A3; D005947:Glucose; D012966:Sodium Cyanide