title: Primary African HIV clade A and D isolates: effective cross-clade neutralization with a quadruple combination of human monoclonal antibodies raised against clade B.
abstract: We investigated the ability of several human neutralizing monoclonal antibodies (nmAbs), originally raised against human immunodeficiency virus (HIV) clade B isolates, to neutralize primary clade A and D isolates as single agents and in combinations. All four primary HIV clade A isolates and five primary HIV clade D isolates tested were neutralized >99% by the quadruple combination of nmAbs IgG1b12, 2G12, 2F5, and 4E10. These mAbs recognize conserved epitopes on HIV-1 envelope (Env), resulting in strong cross-clade neutralization. Previously, we showed synergistic neutralization of primary HIV-1 clade C isolates in vitro by the same nMAb combination. We and others also showed neutralization of primary HIV clade B strains. Together, our data show that the quadruple combination of mAbs effectively neutralized primary HIV clade A, B, C, and D isolates.
authors.affiliation: Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
authors.forename: Moiz
authors.initials: M
authors.lastname: Kitabwalla
authors.forename: Flavia
authors.initials: F
authors.lastname: Ferrantelli
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Alistair
authors.initials: A
authors.lastname: Chalmers
authors.forename: Hermann
authors.initials: H
authors.lastname: Katinger
authors.forename: Gabriela
authors.initials: G
authors.lastname: Stiegler
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Cavacini
authors.forename: Ting-Chao
authors.initials: TC
authors.lastname: Chou
authors.forename: Ruth M
authors.initials: RM
authors.lastname: Ruprecht
mesh_terms: D000349:Africa; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions; D015483:HIV Antibodies / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D009500:Neutralization Tests
chemical_list: D000911:Antibodies, Monoclonal; D015483:HIV Antibodies