title: Inert site in a protein zinc cluster: isotope exchange by high resolution mass spectrometry.
abstract: Using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry, we show that one Zn2+ ion in the Zn4 cluster of cyanobacterial metallothionein is inert to exchange with 67Zn2+. We suggest that this is zinc in site A, which together with the surrounding alpha and beta secondary structure forms a zinc-finger fold.
authors.affiliation: School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, Scotland, U.K.
authors.forename: Claudia A
authors.initials: CA
authors.lastname: Blindauer
authors.forename: Nick C
authors.initials: NC
authors.lastname: Polfer
authors.forename: Stella E
authors.initials: SE
authors.lastname: Keiper
authors.forename: Mark D
authors.initials: MD
authors.lastname: Harrison
authors.forename: Nigel J
authors.initials: NJ
authors.lastname: Robinson
authors.forename: Pat R R
authors.initials: PR
authors.lastname: Langridge-Smith
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Sadler
mesh_terms: D017357:Cyclotrons; D005583:Fourier Analysis; D013058:Mass Spectrometry / Q000379:methods; D008958:Models, Molecular; D015032:Zinc / Q000737:chemistry*
chemical_list: D015032:Zinc