Potato tuber phospholipids contain colneleic acid in the 2-position.
Colneleic acid (9-[1'(E),3'(Z)-nonadienyloxy]-8(E)-nonenoic acid) is produced from linoleic acid by the sequential action of 9-lipoxygenase and divinyl ether synthase. We demonstrate that a small fraction of the colneleic acid in potato tubers is esterified in phospholipids. This colneleic acid was released by chemical hydrolysis and a phospholipase A(2), but not by a lipase with 1-acyl specificity. Phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol contain molecular species with nominal masses consistent with identification as palmitoyl,colneleoyl species. Exact mass analysis of its fragments confirmed the identity of palmitoyl,colneloyl phosphatidylinositol. To our knowledge, this work represents the first identification of a colneleoyl phospholipid.
Fauconnier
Marie-Laure
ML
Unité de Chimie Générale et Organique, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Déportés 2, B-5030 Gembloux, Belgium.
Williams
Todd D
TD
Marlier
Michel
M
Welti
Ruth
R
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D011198:Solanum tuberosum / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; C002946:colneleic acid