title: Enhancement of protease activity involved in modulation of cell mutability by microgravity stress in UVC-irradiated human cells.
abstract: It is an intriguing question whether gravity-changing stress modulates human cell mutability. To resolve this problem, it is necessary to determine the cellular events leading to modulation. We previously detected protease activation just after UV (UVC, principally 254 nm wavelength) irradiation followed by hypomutability in cultured human cells. We here investigated whether UV-activated protease activity is affected in human UVAP-1 cells exposed to gravity-changing stress prior to UV irradiation.
authors.affiliation: Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: H C
authors.initials: HC
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Sugaya
authors.forename: J
authors.initials: J
authors.lastname: Nomura
authors.forename: K
authors.initials: K
authors.lastname: Kita
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
mesh_terms: D002460:Cell Line; D002498:Centrifugation; D003546:Cysteine Endopeptidases / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism* / Q000528:radiation effects*; D006801:Humans; D018471:Hypergravity; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D016296:Mutagenesis / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014893:Weightlessness
chemical_list: D015853:Cysteine Proteinase Inhibitors; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D007930:Leucine; C024974:E 64