Isolation of Escherichia coli O157:H7 from intact colon fecal samples of swine.
Escherichia coli O157:H7 was recovered from colon fecal samples of pigs. Polymerase chain reaction confirmed two genotypes: isolates harboring the eaeA, stx(1), and stx(2) genes and isolates harboring the eaeA, stx(1), and hly(933) genes. We demonstrate that swine in the United States can harbor potentially pathogenic E. coli O157:H7.
Feder
Ingrid
I
Microbial Biophysics and Residue Chemistry and Core Technologies, U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania 19038, USA. ifeder@arserrc.gov
Wallace
F Morgan
FM
Gray
Jeffrey T
JT
Fratamico
Pina
P
Fedorka-Cray
Paula J
PJ
Pearce
Rachel A
RA
Call
Jeffrey E
JE
Perrine
Richard
R
Luchansky
John B
JB
D000818:Animals; D016521:Electrophoresis, Gel, Pulsed-Field; D019453:Escherichia coli O157 / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D013552:Swine / Q000382:microbiology*