High levels of hyaluronan production by a malignant lymphoma cell line with primary effusion lymphoma immunophenotype OHK.
We investigated whether hyaluronan (HA) was produced by a malignant lymphoma cell line with a primary effusion lymphoma immunophenotype, OHK. OHK secreted high levels of HA into the culture supernatant, and reverse transcription polymerase chain reaction showed mRNA for the HA synthases 2 and 3. HA binding protein-reactive HA was present in the plasma membrane and in the cytoplasm. The inoculation of OHK into severe combined immunodeficiency disease mice produced marked viscid ascites with concentrated HA and a vascular-rich mesenteric solid tumour. These results show that HA is produced and secreted by the malignant lymphoma cell line OHK.
Kuwabara
Hiroko
H
Department of Pathology, Osaka Medical College, Osaka, Japan. pa2020@art.osaka-med.ac.jp
Yoneda
Masahiko
M
Nagai
Masami
M
Nishio
Hajime
H
Tasaka
Taizo
T
Suzuki
Koichi
K
Mori
Hiroshi
H
D000818:Animals; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism*; D016130:Immunophenotyping; D008223:Lymphoma / Q000276:immunology / Q000503:physiopathology*; D051379:Mice; D016513:Mice, SCID; D009374:Neoplasms, Experimental; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D014407:Tumor Cells, Cultured
D019408:Platelet Endothelial Cell Adhesion Molecule-1; D006820:Hyaluronic Acid