Purification of the 67-kDa lectin-like glycoprotein of Trypanosoma cruzi, LLGP-67, and its evaluation as a relevant antigen for the diagnosis of human infection.
In the present work we propose a simple method for affinity purification of the 67-kDa lectin-like glycoprotein (LLGP-67) from Trypanosoma cruzi, the causative agent of Chagas' disease. The LLGP-67, which presents galactose binding activity and participates in the host cell recognition process, was previously purified by methods based on its interaction with galactose residues on erythrocytic membranes. We describe herein results showing that this protein can be purified from T. cruzi in a direct way using non-derivatized agarose as a chromatographic ligand. We also demonstrate the relevance of LLGP-67 as an antigen for human diagnosis of chagasic infection. Sensitivity and specificity for this antigen were calculated, being 98 and 98.11% respectively.
Marcipar
Iván S
IS
INTEBIO, Facultad de Bioqui;mica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, Paraje El Pozo, C. C. 242 (3000) Santa Fe, Argentina.
Welchen
Elina
E
Roodveldt
Cintia
C
Marcipar
Alberto J
AJ
Silber
Ariel M
AM
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D014355:Chagas Disease / Q000175:diagnosis* / Q000469:parasitology; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005690:Galactose / Q000378:metabolism; D006801:Humans; D007106:Immune Sera; D037102:Lectins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D015800:Protozoan Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000276:immunology
D000953:Antigens, Protozoan; D007106:Immune Sera; D037102:Lectins; D015800:Protozoan Proteins; D005690:Galactose