title: Binding of antibromelain monomeric Fab' improves the stability of stem bromelain against inactivation.
abstract: Antienzyme polyclonal antibodies against stem bromelain were raised in male albino rabbits and the Fab' monomers isolated from the IgG of the immune sera. Incubation of bromelain with the Fab' resulted in binding and gel filtration of the resulting complex suggested a 1:1 stoichiometry. Complexing with the Fab' resulted in significant stabilization of bromelain against thermal inactivation and alkaline pH.
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Uttar Pradesh, India.
authors.forename: Pawan
authors.initials: P
authors.lastname: Gupta
authors.forename: Rizwan H
authors.initials: RH
authors.lastname: Khan
authors.forename: M
authors.initials: M
authors.lastname: Saleemuddin
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000737:chemistry; D001963:Bromelains / Q000008:administration & dosage / Q000276:immunology*; D004795:Enzyme Stability / Q000276:immunology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007140:Immunoglobulin Fab Fragments / Q000276:immunology* / Q000302:isolation & purification; D008297:Male; D011817:Rabbits
chemical_list: D000936:Antigen-Antibody Complex; D007140:Immunoglobulin Fab Fragments; D001963:Bromelains; C091760:stem bromelain