Quantitative HPLC analysis of withanolides in Withania somnifera.
One of the most widely used herbs in Ayurvedic medicine is Ashwaghanda, Withania somnifera, a shrub commonly found on the Indian subcontinent. As this plant is increasingly becoming a popular adaptogenic in the western world, analytical methods for its identification and quality control are in demand. Thus, a HPLC method for the determination of withaferin A and withanolide D was developed. The system was successfully used to investigate the presence of the markers in different W. somnifera plant parts as well as to analyze their content in market products.
Ganzera
M
M
Department of Pharmacognosy, University of Innsbruck, Innsbruck 6020, Austria. markus.ganzera@uibk.ac.at
Choudhary
M I
MI
Khan
I A
IA
D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards*; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000737:chemistry*; D006801:Humans; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves; D018517:Plant Roots; D018547:Plant Stems; D015203:Reproducibility of Results; D032341:Withania; D054358:Withanolides
D010936:Plant Extracts; D054358:Withanolides; C010376:withanolide D; C009684:withaferin A; D004875:Ergosterol