Efficient adeno-associated virus-mediated gene expression in human placenta-derived mesenchymal cells.
Mesenchymal cells from various sources are pluripotent and are attractive sources for cell transplantation. In this study, we analyzed recombinant adeno-associated virus (rAAV)-mediated gene expression in human placenta-derived mesenchymal cells (hPDMCs), which reside in placental villi. After transduction of AV-CAG-EGFP, a rAAV expressing enhanced green fluorescence protein (EGFP), hPDMCs showed much higher level of EGFP expression than human umbilical vein endothelial cells or rat aortic smooth muscle cells. The number of EGFP-positive hPDMCs infected by AV-CAG-EGFP alone did not increase significantly by coinfection of adenovirus, which enhanced expression level of the rAAV vector. Moreover, flow cytometric analysis showed discrete positive fraction of EGFP-expressing hPDMCs, which is about 15-20% of the cells infected with AV-CAG-EGFP. Therefore, some cell population in hPDMCs might be highly susceptible to rAAV-mediated gene transduction. In addition, stable EGFP expressions were observed in about 1% of hPDMCs infected with AV-CAG-EGFP at 4 weeks post-infection. Collectively, hPDMCs have characters favorable for rAAV-mediated gene expression.
Zhang
Xiaohong
X
Department of Advanced Medical Science, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
Nakaoka
Takashi
T
Nishishita
Toshihide
T
Watanabe
Nobukazu
N
Igura
Koichi
K
Shinomiya
Ken-ichi
K
Takahashi
Tsuneo A
TA
Yamashita
Naohide
N
D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D000229:Dependovirus / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D015967:Gene Expression Regulation, Viral; D015316:Genetic Therapy; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D008648:Mesoderm; D032389:Myocytes, Smooth Muscle / Q000276:immunology / Q000821:virology; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000276:immunology / Q000378:metabolism / Q000821:virology*; D011247:Pregnancy; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D014161:Transduction, Genetic / Q000379:methods*
D004279:DNA, Viral; D008164:Luminescent Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins