Solid-phase clean-up in the liquid chromatographic determination of polycyclic aromatic hydrocarbons in edible oils.
A solid-phase extraction (SPE) method for sample clean-up, followed by reversed-phase high-performance liquid chromatography (HPLC) with fluorescence detection is reported for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible oils. The effects of experimental variables, such as washing and elution solvents, sample solvent and drying time have been studied using C18 cartridges. Recoveries and selectivity using other sorbent materials (C8, C2, CH, PH and NH2) were also examined, with C18 being the best one. The recoveries ranged between 50 and 103% depending on the molecular mass of the PAH. The limits of quantitation were lower than 1 ng/g for most PAHs and good precision was achieved. The method was validated using certified reference materials.
Barranco
A
A
Department of Analytical Chemistry, Faculty of Sciences, University of the Basque Country, P.O. Box 644, E-48080 Bilbao, Spain.
Alonso-Salces
R M
RM
Bakkali
A
A
Berrueta
L A
LA
Gallo
B
B
Vicente
F
F
Sarobe
M
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009821:Oils / Q000737:chemistry*; D011083:Polycyclic Compounds / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D009821:Oils; D011083:Polycyclic Compounds