Autoimmune hair loss induced by alloantigen in C57BL/6 mice.
Exponentially growing Meth-A cells expressing H-2K(d).D (d) antigen were found to induce alopecia when injected intraperitoneally into normal C57BL/6 mice, which express the H-2K(b).D (b) antigen. However, the capacity to induce alopecia disappeared when Meth-A cells were treated with K252a, which inhibits protein kinases. Histologically, skin in affected areas showed dense mononuclear cell infiltration and a focal foreign-body giant-cell reaction in hair follicles. The subtyping of lymphocytes in peripheral blood demonstrated a significant difference between normal mice and Meth-A cell-injected mice. To further examine the mechanism by which the alloantigen induces alopecia, lymphocytes isolated from the peripheral blood of normal C57BL/6 mice were cultured in medium containing Meth-A cell homogenate, phytohemagglutinin (PHA) and recombinant mouse interleukin-2 (rm IL-2), and intravenously injected into normal C57BL/6 mice. The adoptive transfer of the lymphocytes induced alopecia in a similar way. These findings suggest that the protein kinase-modulated alloantigen induces alopecia by disturbing the immunological homeostasis, and that lymphokine-activated killer cells play an important role in induction of alopecia by cross-reacting with hair follicles.
Zong
Zhi-ping
ZP
Department of Serology, Kanazawa Medical University, Uchinada-machi, Ishikawa-ken 920-0293, Japan. zong3961@kanazawa-med.ac.jp
Matsui
Shinobu
S
Li
Ai-li
AL
Katsuda
Shogo
S
Yamaguchi
Nobuo
N
D019264:Adoptive Transfer; D000505:Alopecia / Q000235:genetics* / Q000276:immunology*; D000818:Animals; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005260:Female; D018859:Hair Follicle / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D006367:HeLa Cells; D006706:Homeostasis / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007519:Isoantigens / Q000276:immunology*; D015979:Killer Cells, Lymphokine-Activated / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D010835:Phytohemagglutinins / Q000494:pharmacology; D011494:Protein Kinases / Q000187:drug effects / Q000378:metabolism*
D017077:Culture Media, Conditioned; D007376:Interleukin-2; D007519:Isoantigens; D010835:Phytohemagglutinins; D011494:Protein Kinases