Proton transfer reactions in the F86D and F86E mutants of pharaonis phoborhodopsin (sensory rhodopsin II).
pharaonis phoborhodopsin (ppR, also called pharaonis sensory rhodopsin II, psRII), a negative phototaxis receptor of Natronobacterium pharaonis, can use light to pump a proton in the absence of its transducer protein. However, the pump activity is much lower than that of the light-driven proton-pump bacteriorhodopsin (BR). ppR's pump activity is known to be increased in a mutant protein, in which Phe86 is replaced with Asp (F86D). Phe86 is the amino acid residue corresponding to Asp96 in BR, and we expect that Asp86 plays an important role in the proton transfer at the highly hydrophobic cytoplasmic domain of the F86D mutant ppR. In this article, we studied protein structural changes and proton transfer reactions during the photocycles of the F86D and F86E mutants in ppR by means of Fourier transform infrared (FTIR) spectroscopy and photoelectrochemical measurements using a tin oxide (SnO2) electrode. FTIR spectra of the unphotolyzed state and the K and M intermediates are very similar among F86D, F86E, and the wild type. Asp86 or Glu86 is protonated in F86D or F86E, respectively, and the pK(a) > 9. During the photocycle, the pK(a) is lowered and deprotonation of Asp86 or Glu86 is observed. Detection of both deprotonation of Asp86 or Glu86 and concomitant reprotonation of the 13-cis chromophore implies the presence of a proton channel between position 86 and the Schiff base. However, the photoelectrochemical measurements revealed proton release presumably from Asp86 or Glu86 to the cytoplasmic aqueous phase in the M state. This indicates that the ppR mutants do not have the BR-like mechanism that conducts a proton uniquely from Asp86 or Glu86 (Asp96 in BR) to the Schiff base, which is possible in BR by stepwise protein structural changes at the cytoplasmic side. In ppR, there is a single open structure at the cytoplasmic side (the M-like structure), which is shown by the lack of the N-like protein structure even in F86D and F86E at alkaline pH. Therefore, it is likely that a proton can be conducted in either direction, the Schiff base or the bulk, in the open M-like structure of F86D and F86E.
Iwamoto
Masayuki
M
Laboratory of Biophysical Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.
Furutani
Yuji
Y
Kamo
Naoki
N
Kandori
Hideki
H
D019943:Amino Acid Substitution / Q000235:genetics*; D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics; D001224:Aspartic Acid / Q000235:genetics; D002338:Carotenoids / Q000737:chemistry* / Q000235:genetics; D004563:Electrochemistry; D004579:Electron Transport / Q000235:genetics; D005615:Freezing; D018698:Glutamic Acid / Q000235:genetics; D025602:Halorhodopsins; D019633:Natronobacterium / Q000737:chemistry* / Q000235:genetics; D010649:Phenylalanine / Q000235:genetics; D010777:Photochemistry; D017494:Proton Pumps / Q000737:chemistry* / Q000235:genetics; D025603:Sensory Rhodopsins; D017550:Spectroscopy, Fourier Transform Infrared
D019843:Archaeal Proteins; D025602:Halorhodopsins; D017494:Proton Pumps; D025603:Sensory Rhodopsins; C061508:sensory rhodopsin II protein, archaeal; D001224:Aspartic Acid; D002338:Carotenoids; D018698:Glutamic Acid; D010649:Phenylalanine