title: Jenfluor ap--a novel fluorogenic substrate for in situ detection of alkaline phosphatase activity.
abstract: Alkaline phosphatase (AP) activity is often targeted in enzyme-related histochemistry as probe enzyme to detect neoplastic cells, as marker for primordial germ cells as well as in preimplantation studies, osteoblast differentiation, phosphate starvation in bacteria, yeast and phytoplankton. Moreover, AP-marker activity is a very useful tool in immunohistochemistry to detect gene sequences, antigens and antibodies. Here we describe a novel high resolution fluorescence method to localize AP-activity in cells and tissue sections based on a naphthol-AS azo coupling procedure (Jenfluor ap). This method provides amorphous photostable fluorescent final reaction products without any diffusion artifacts which are visible in conventional fluorescence microscopes as well as in confocal laser scanning and near infrared multiphoton laser scanning microscopes. The superiority of the Jenfluor ap method in comparison to the known Fast Red TR salt as well as the ELF stains is discussed.
authors.affiliation: Friedrich Schiller University of Jena, Institute of Anatomy II, Teichgraben 7, 07743 Jena, Germany. khal@mti-n.uni-jena.de
authors.forename: Karl-Jürgen
authors.initials: KJ
authors.lastname: Halbhuber
authors.forename: Reimar
authors.initials: R
authors.lastname: Krieg
authors.forename: Peter
authors.initials: P
authors.lastname: Fischer
authors.forename: Karsten
authors.initials: K
authors.lastname: König
authors.forename: Hubert
authors.initials: H
authors.lastname: Nasse
authors.forename: Wolfram
authors.initials: W
authors.lastname: Dietz
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers; D004355:Drug Stability; D004822:Epididymis / Q000201:enzymology; D005260:Female; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D007700:Kinetics; D008297:Male; D009130:Muscle, Smooth / Q000201:enzymology; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015415:Biomarkers; D005456:Fluorescent Dyes; D000469:Alkaline Phosphatase