The myth of ventrally emigrating neural tube (VENT) cells and their contribution to the developing cardiovascular system.
The cardiac neural crest cells are a group of cells that emigrate from the dorsal side of the neural tube during a specific time window and contribute to the pharyngeal arch arteries and the aorticopulmonary septum of the heart. Recent publications have suggested that another group of cells emigrating from the ventral side of the neural tube also contributes to the developing cardiovascular system. The first aim of our study was to define the specific time window of cardiac neural crest cell migration by injecting a retrovirus containing a lacZ reporter gene into a chick embryo at different stages during development. The second aim was to study the contribution of the supposed ventrally emigrating neural tube cells to the cardiovascular system using three approaches. One approach was to inject a lacZ retrovirus into the lumen of the chick hindbrain. Secondly, we injected the retrovirus into the neural tube at the position of the 10-12 somite pair. Finally, we used the chimera technique in which we transplanted a quail neural tube segment into a chick embryo. Cardiac neural crest cells were shown to emigrate from the dorsal side of the neural tube between HH9 and HH13(-). The HH13(+) neural tube has ceased to produce cardiac neural crest cells between the level of the otic placode and the fourth pair of somites. Retroviral injection directly into the chick hindbrain at HH14 resulted in 50% of the embryos with minimal labeling of the hindbrain and intense labeling of the adjacent mesenchyme, suggesting that virus was spilled. This implies that this technique is not useful for confirming the existence of ventrally emigrating cells. Both retroviral injections into the neural tube lumen at HH14 at the position of the 10-12 somite pair and the chimeras showed no signs of ventrally emigrating neural tube cells. We conclude that there is no contribution of ventral neural tube cells to the developing cardiovascular system.
Boot
Marit J
MJ
Department of Anatomy and Embryology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands.
Gittenberger-de Groot
Adriana C
AC
van Iperen
Liesbeth
L
Poelmann
Robert E
RE
D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002642:Chick Embryo; D002678:Chimera; D003370:Coturnix; D017930:Genes, Reporter; D006321:Heart / Q000196:embryology*; D007150:Immunohistochemistry; D007763:Lac Operon; D009024:Morphogenesis; D009432:Neural Crest / Q000166:cytology / Q000196:embryology*; D013047:Specific Pathogen-Free Organisms; D013997:Time Factors