Migration and differentiation of myogenic precursors following transplantation into the developing rat brain.
There is increasing evidence that muscle-derived precursor cells can, under appropriate conditions, give rise to other than myogenic cell types. Transplantation into the embryonic ventricular zone provides a unique opportunity to study the migration and differentiation of non-neural somatic progenitor cells in response to instructive cues within the developing neuroepithelium. Here, we demonstrate that myogenic cell lines grafted into the ventricles of rat embryos showed widespread migration into several host brain compartments. In contrast to incorporation patterns observed after transplantation of neural cells, grafted myoblasts incorporated virtually exclusively along endogenous blood vessels. Preferential incorporation sites included cortex, olfactory bulb, hippocampus, striatum, thalamus, hypothalamus, and tectum. While the engrafted myoblasts showed no evidence of neural differentiation, a fraction exhibited pronounced coexpression of endothelial marker antigens. These findings support the concept of a close developmental relationship between the myogenic and the endothelial lineages. Used as a delivery system, transfected myoblasts may be exploited for widespread gene transfer to the perivascular compartment of the perinatal central nervous system.
Steffel
Jan
J
Institute of Reconstructive Neurobiology, University of Bonn, Germany.
Wernig
Marius
M
Knauf
Ulrich
U
Kumar
Santhosh
S
Wiestler
Otmar D
OD
Wernig
Anton
A
Brüstle
Oliver
O
D000818:Animals; D015415:Biomarkers; D001921:Brain / Q000098:blood supply / Q000254:growth & development / Q000601:surgery*; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D002465:Cell Movement; D002678:Chimera; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D018014:Gene Transfer Techniques; D007763:Lac Operon; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology*; D032446:Myoblasts / Q000166:cytology / Q000637:transplantation*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D019076:Transgenes
D015415:Biomarkers