title: Pulsatilloside A and anemoside A3 protect PC12 cells from apoptosis induced by sodium cyanide and glucose deprivation.
abstract: Using sodium cyanide (NaCN) and glucose deprivation induced cell injury in PC12 as an injury model, we investigated the protective effects of pulsatilloside A and anemoside A 3 on neurons. The results showed that PC12 cells under the NaCN-injury and glucose deprivation would undergo apoptosis. Additions of pulsatilloside A and anemoside A 3, at dosages ranging from 0.1, 1 and 10 microg/ml, protected PC12 cells from apoptosis determined by MTT, LDH release analysis, and flow cytometry measurement.
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Gao
authors.forename: Wen-Cai
authors.initials: WC
authors.lastname: Ye
authors.forename: Albert C H
authors.initials: AC
authors.lastname: Yu
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ren-Xiang
authors.initials: RX
authors.lastname: Tan
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: W L Wendy
authors.initials: WL
authors.lastname: Hsiao
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005947:Glucose; D006801:Humans; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D016716:PC12 Cells / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D031946:Pulsatilla; D051381:Rats; D012503:Saponins; D012966:Sodium Cyanide; D014315:Triterpenes
chemical_list: D018696:Neuroprotective Agents; D010936:Plant Extracts; C473641:Pulsatilloside A; D012503:Saponins; D014315:Triterpenes; C473640:anemoside A3; D005947:Glucose; D012966:Sodium Cyanide