Purification of normal lymphocytes from leukemic T-cells by lectin-affinity adsorbents - correlation with lectin-cell binding.
Utilization of leukemic T-cells from normal ones, using lectin-affinity adsorbents, is described. CNBr-activated Sepharose 6MB was covalently coupled to Soybean (SBA) or Dolichos Biflorus Agglutinins (DBA), then serves as an affinity probe for separation of leukemic T-cells from normal lymphocytes. The normal lymphocytes were removed almost completely by phosphate buffered saline (Ca(2+) and Mg(2+) free) (PBS(-)) from lectin-affinity column. More than 80% of the leukemic T-cells were retained on the lectin-affinity adsorbent, whereas another 10-15% were easily removed by PBS(-). There was a very good linear correlation between percent of cells, retained on the lectin-affinity adsorbent and percent of cells, interacting with the respective free lectin (r=0.97 for SBA, and r=0.93 for DBA). The viability of normal lymphocytes was not influenced after passing through the columns. In the case of leukemic T-cells - about 90% of the easily removed cells were dead, and another 10% were viable cells, non-interacting with DBA or SBA.
Bakalova
R
R
Institute for Structural and Engineering Materials, Independent Administrative Institution, National Institute of Advanced Industrial Science and Technology, AIST-Kyushu, Saga-ken, Japan. r.bakalova-zheleva@aist.go.jp
Ohba
H
H
D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002846:Chromatography, Affinity / Q000379:methods*; D005434:Flow Cytometry; D006801:Humans; D007938:Leukemia / Q000276:immunology* / Q000473:pathology*; D037121:Plant Lectins / Q000378:metabolism*; D030262:Soybean Proteins / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured; D010835:Phytohemagglutinins
D037121:Plant Lectins; D030262:Soybean Proteins; C036554:dolichos biflorus agglutinin; C028824:soybean lectin; D010835:Phytohemagglutinins