title: Molecular characterization of Oenococcus oeni genes encoding proteins involved in arginine transport.
abstract: OBJECTIVE
  This work was carried out to complete the sequence of the arc cluster involved in arginine catabolism in Oenococcus oeni, and particularly to characterize the genes encoding proteins involved in arginine transport.
  
  
  RESULTS
  Using molecular cloning, two loci encoding proteins involved in the arginine-ornithine antiport were isolated. Their expression patterns were monitored by RT-PCR to study the influence of arginine on their transcription. Polycistronic mRNAs were detected. PCR performed directly on colonies with primer pairs specific of arc genes was used to discriminate strains able/unable to degrade arginine.
  
  
  CONCLUSIONS
  Oenococcus oeni contains two arcD loci encoding similar proteins. Their expression is not influenced by arginine and polycistronic messengers were detected. The inability to use arginine is due to a lack of genetic information encoding proteins of the arginine deiminase pathway.
  
  
  CONCLUSIONS
  The constitutive expression of arcD genes points to the positive role of arginine on O. oeni cell growth. The occasional presence of all the arc ABCD genes together in O. oeni strains might provide insights into the growth rate variability within this species.
authors.affiliation: Laboratoire de Biotechnologie et Microbiologie Appliquée, Faculté d'Oenologie, Unité Associée INRA-Université Victor Ségalen Bordeaux 2, Talence, France.
authors.forename: B
authors.initials: B
authors.lastname: Divol
authors.forename: T
authors.initials: T
authors.lastname: Tonon
authors.forename: S
authors.initials: S
authors.lastname: Morichon
authors.forename: E
authors.initials: E
authors.lastname: Gindreau
authors.forename: A
authors.initials: A
authors.lastname: Lonvaud-Funel
mesh_terms: D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems / Q000235:genetics* / Q000378:metabolism; D017920:Antiporters / Q000235:genetics* / Q000378:metabolism; D001120:Arginine / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001692:Biological Transport / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006095:Gram-Positive Cocci / Q000235:genetics* / Q000378:metabolism; D007934:Leuconostoc / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012329:RNA, Bacterial / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D026905:Amino Acid Transport Systems; D017920:Antiporters; C063930:ArcD protein, Bacteria; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D001120:Arginine