SAS-4 is essential for centrosome duplication in C elegans and is recruited to daughter centrioles once per cell cycle.
The mechanisms governing centrosome duplication remain poorly understood. We identified a gene called sas-4 that is essential for this process in C. elegans. SAS-4 encodes a predicted coiled-coil protein that localizes to a tiny dot in the center of centrosomes throughout the cell cycle. FRAP experiments with GFP-SAS-4 transgenic embryos reveal that SAS-4 is recruited to the centrosome once per cell cycle, at the time of organelle duplication. Additional evidence indicates that SAS-4 is recruited to the daughter centriole or a closely associated structure. These findings identify SAS-4 recruitment as a key step in the centrosome duplication cycle.
Leidel
Sebastian
S
Swiss Institute for Experimental, Cancer Research (ISREC), CH-1066 Epalinges/, Lausanne, Switzerland.
Gönczy
Pierre
P
D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics*; D002502:Centrioles / Q000235:genetics / Q000378:metabolism; D018385:Centrosome / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D005260:Female; D031541:Fluorescence Resonance Energy Transfer; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000235:genetics; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D008297:Male; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000172:deficiency / Q000235:genetics; D034622:RNA Interference / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism
D029742:Caenorhabditis elegans Proteins; D008164:Luminescent Proteins; C472409:SAS-4 protein, C elegans; D049452:Green Fluorescent Proteins; D011494:Protein Kinases; C429649:zyg-1 protein, C elegans