Changes in the concentration of trigonelline in a semi-arid leguminous plant (Prosopis laevigata) induced by an arbuscular mycorrhizal fungus during the presymbiotic phase.
An in vitro presymbiotic system between mesquite [Prosopis laevigata(Willd.) M.C. Johnst], a semi-arid leguminous plant, and pregerminated spores of Gigaspora rosea Nicol. & Schenck was established. After characteristic hyphal branching, high performance liquid chromatographic analyses of methanol extracts from P. laevigata roots revealed a concentration change in one ultraviolet-detectable product. This product was identified by nuclear magnetic resonance and mass spectrometry as trigonelline, a pyridine alkaloid. The concentration of trigonelline was constant in the aerial parts of the plant with or without G. rosea, but its concentration in the roots increased 1.8-fold when G. roseawas present. Trigonelline may be a regulatory factor during early signal events in the establishment of the arbuscular mycorrhizal symbiosis in P. laevigata.
Rojas-Andrade
R
R
Departamento de Biotecnología y Bioingeniería, CINVESTAV, Avenida Instituto Politécnico Nacional 2508, C.P. 07360 Mexico D.F., Mexico.
Cerda-García-Rojas
C M
CM
Frías-Hernández
J T
JT
Dendooven
L
L
Olalde-Portugal
V
V
Ramos-Valdivia
A C
AC
D000470:Alkaloids / Q000032:analysis*; D005658:Fungi / Q000502:physiology; D038821:Mycorrhizae / Q000502:physiology*; D018517:Plant Roots / Q000737:chemistry / Q000382:microbiology; D029862:Prosopis / Q000737:chemistry* / Q000382:microbiology / Q000502:physiology; D013559:Symbiosis / Q000502:physiology
D000470:Alkaloids; C009560:trigonelline