title: A cell shrinkage-induced non-selective cation conductance with a novel pharmacology in Ehrlich-Lettre-ascites tumour cells.
abstract: In whole-cell recordings on Ehrlich-Lettre-ascites tumour (ELA) cells, the shrinkage-induced activation of a cation conductance with a selectivity ratio P(Na):P(Li):P(K):P(choline):P(NMDG) of 1.00:0.97:0.88:0.03:0.01 was observed. In order of potency, this conductance was blocked by Gd(3+)=benzamil>amiloride>ethyl-isopropyl-amiloride (EIPA). In patch-clamp studies using the cell-attached configuration, a 14 pS channel became detectable that was reversibly activated upon hypertonic cell shrinkage. It is concluded that ELA cells express a shrinkage-induced cation channel that may reflect a molecular link between amiloride-sensitive and -insensitive channels. In addition, because of its pharmacological profile, it may possibly be related to epithelial Na+ channels (ENaCs).
authors.affiliation: Max-Planck-Institut für molekulare Physiologie, Otto-Hahn-Strasse 11, D-44227 Dortmund, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Lawonn
authors.forename: Else K
authors.initials: EK
authors.lastname: Hoffmann
authors.forename: Charlotte
authors.initials: C
authors.lastname: Hougaard
authors.forename: Frank
authors.initials: F
authors.lastname: Wehner
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002412:Cations; D048429:Cell Size; D004553:Electric Conductivity; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D014407:Tumor Cells, Cultured
chemical_list: D002412:Cations; D007473:Ion Channels