title: Antizyme is a target of sex-lethal in the Drosophila germline and appears to act downstream of hedgehog to regulate sex-lethal and cyclin B.
abstract: The sex determination master switch, Sex-lethal, has been shown to regulate the mitosis of early germ cells in Drosophila melanogaster. Sex-lethal is an RNA binding protein that regulates splicing and translation of specific targets in the soma, but the germline targets are unknown. In an experiment aimed at identifying targets of Sex-lethal in early germ cells, the RNA encoded by gutfeeling, the Drosophila homolog of Ornithine Decarboxylase Antizyme, was isolated. gutfeeling interacts genetically with Sex-lethal. It is not only a target of Sex-lethal, but also appears to regulate the nuclear entry and overall levels of Sex-lethal in early germ cells. This regulation of Sex-lethal by gutfeeling appears to occur downstream of the Hedgehog signal. We also show that Hedgehog, Gutfeeling, and Sex-lethal function to regulate Cyclin B, providing a link between Sex-lethal and mitosis.
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Vied
authors.forename: Naomi
authors.initials: N
authors.lastname: Halachmi
authors.forename: Adi
authors.initials: A
authors.lastname: Salzberg
authors.forename: Jamila I
authors.initials: JI
authors.lastname: Horabin
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D019926:Cyclin B / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D053823:Hedgehog Proteins; D006358:Hot Temperature; D008297:Male; D008954:Models, Biological; D009154:Mutation; D009866:Oogenesis / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics*; D019849:Sex Determination Processes; D012733:Sex Differentiation / Q000235:genetics
chemical_list: C503698:CycB protein, Drosophila; D019926:Cyclin B; D029721:Drosophila Proteins; D053823:Hedgehog Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; C067777:Sxl protein, Drosophila; C031017:ornithine decarboxylase antizyme; C077245:hh protein, Drosophila; D012313:RNA