title: Decreased expression of adrenomedullin during adipocyte-differentiation of 3T3-L1 cells.
abstract: Adrenomedullin (AM) is a potent vasodilator peptide which has an inhibitory action on insulin secretion. Resistin is a novel peptide specifically secreted from adipocytes, and implicated in insulin resistance. We studied the expression of AM and resistin in 3T3-L1 adipocytes and preadipocytes by Northern blot analysis and radioimmunoassay. Immunoreactive-AM was detected in the culture media of 3T3-L1 preadipocytes and adipocytes, with higher concentrations found in preadipocytes. Northern blot analysis showed that AM mRNA was expressed in 3T3-L1 preadipocytes but was undetectable in adipocytes. In contrast, resistin mRNA was expressed in 3T3-L1 adipocytes, whereas it was not detected in 3T3-L1 preadipocytes. The present study thus showed that AM expression was decreased, and resistin expression increased, during adipocyte-differentiation of 3T3-L1 cells.
authors.affiliation: Department of Molecular Biology and Applied Physiology, Tohoku University School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575 Japan.
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Totsune
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Takeda
authors.forename: Kazumichi
authors.initials: K
authors.lastname: Furuyama
authors.forename: Shigeki
authors.initials: S
authors.lastname: Shibahara
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology* / Q000502:physiology*; D053607:Adrenomedullin; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D015870:Gene Expression / Q000502:physiology; D006729:Hormones, Ectopic / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D020932:Nerve Growth Factor; D010455:Peptides / Q000235:genetics*; D011506:Proteins; D012333:RNA, Messenger / Q000032:analysis; D011863:Radioimmunoassay; D052243:Resistin; D013234:Stem Cells / Q000166:cytology / Q000502:physiology
chemical_list: D006729:Hormones, Ectopic; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011506:Proteins; D012333:RNA, Messenger; D052243:Resistin; C499623:Retn protein, mouse; C413576:Retnla protein, mouse; D053607:Adrenomedullin; D020932:Nerve Growth Factor