title: Bias in dietary-report instruments and its implications for nutritional epidemiology.
abstract: OBJECTIVE
  To evaluate measurement error structure in dietary assessment instruments and to investigate its implications for nutritional studies, using urinary nitrogen excretion as a reference biomarker for protein intake.
  
  
  METHODS
  The dietary assessment methods included different food-frequency questionnaires (FFQs) and such conventional dietary-report reference instruments as a series of 24-hour recalls, 4-day weighed food records or 7-day diaries.
  
  
  METHODS
  Six original pilot validation studies within the European Prospective Investigation of Cancer (EPIC), and two validation studies conducted by the British Medical Research Council (MRC) within the Norfolk cohort that later joined as a collaborative component cohort of EPIC.
  
  
  METHODS
  A sample of approximately 100 to 200 women and men, aged 35-74 years, from each of eight validation studies.
  
  
  RESULTS
  In assessing protein intake, all conventional dietary-report reference methods violated the critical requirements for a valid reference instrument for evaluating, and adjusting for, dietary measurement error in an FFQ. They displayed systematic bias that depended partly on true intake and partly was person-specific, correlated with person-specific bias in the FFQ. Using the dietary-report methods as reference instruments produced substantial overestimation (up to 230%) of the FFQ correlation with true usual intake and serious underestimation (up to 240%) of the degree of attenuation of FFQ-based log relative risks.
  
  
  CONCLUSIONS
  The impact of measurement error in dietary assessment instruments on the design, analysis and interpretation of nutritional studies may be much greater than has been previously estimated, at least regarding protein intake.
authors.affiliation: Biometry Research Group, Division of Cancer Prevention, National Cancer Institute, Bethesda, MD 20892-7354, USA. vk3b@nih.gov
authors.forename: Victor
authors.initials: V
authors.lastname: Kipnis
authors.forename: Douglas
authors.initials: D
authors.lastname: Midthune
authors.forename: Laurence
authors.initials: L
authors.lastname: Freedman
authors.forename: Sheila
authors.initials: S
authors.lastname: Bingham
authors.forename: Nicholas E
authors.initials: NE
authors.lastname: Day
authors.forename: Elio
authors.initials: E
authors.lastname: Riboli
authors.forename: Pietro
authors.initials: P
authors.lastname: Ferrari
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Carroll
mesh_terms: D000328:Adult; D000368:Aged; D015982:Bias; D004032:Diet; D004034:Diet Surveys; D004044:Dietary Proteins / Q000008:administration & dosage* / Q000378:metabolism; D005060:Europe; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D009584:Nitrogen / Q000652:urine*; D009752:Nutritional Status; D015203:Reproducibility of Results
chemical_list: D004044:Dietary Proteins; D009584:Nitrogen