Confocal microscopy of Aspergillus fumigatus keratitis.
OBJECTIVE
To use a confocal microscope to characterise the treated and untreated courses of fungal keratitis.


METHODS
In the first experiment, Aspergillus fumigatus stromal keratitis was produced in both eyes of seven New Zealand white rabbits. In the second experiment, keratitis was induced in right eyes of 20 rabbits. Group 1 rabbits were treated with topical fluconazole, group 2 rabbits received oral fluconazole, and group 3 rabbits were used as controls. The rabbits were examined with a slit lamp and confocal microscope 2, 6, 10, 14, and 20 days after inoculation. The corneal cultures were taken on days 2, 14, and 20 and biopsies were taken on days 2 and 22.


RESULTS
On days 14 and 22 confocal microscopy was more sensitive than culture technique in both treated and untreated animals, since not all cases of fungal keratitis can be cultured.


CONCLUSIONS
This study indicates that confocal microscopy is a rapid and sensitive diagnostic tool for both the early diagnosis and non-invasive follow up of fungal keratitis
Avunduk
A M
AM
Karadeniz Technical University Medical School, Trabzon, Turkey Louisiana State University Eye Center, New Orleans, LA, USA. avunduk@ttnet.net.tr
Beuerman
R W
RW
Varnell
E D
ED
Kaufman
H E
HE
D000284:Administration, Oral; D000287:Administration, Topical; D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage; D001228:Aspergillosis / Q000188:drug therapy / Q000473:pathology*; D001232:Aspergillus fumigatus / Q000302:isolation & purification*; D002478:Cells, Cultured; D003315:Cornea / Q000382:microbiology / Q000473:pathology; D015725:Fluconazole / Q000008:administration & dosage; D007634:Keratitis / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology*; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods*; D011817:Rabbits
D000935:Antifungal Agents; D015725:Fluconazole