[Lectin of Yersinia pestis vaccine strain EV and its immunological properties].
As the result of the chromatographic separation of Y. pestis EV membrane proteins, a protein fraction with hemagglutinating activity was obtained. The isolated preparation was glycoprotein with a molecular weight of 22 kD, contained 16% of carbohydrates and exhibited thermolabile properties. The determination of the carbohydrate specificity of this glycoprotein revealed that it belonged to the class of lectins. Changes in the content of 11 corticosteroids and the population composition of lymphocytes, as well as the detection of specific antibodies in the blood serum of guinea pigs immunized with lectin, were indicative of the fact that the preparation was sufficiently immunogenic and induced the activation of the processes of proliferation and activation of lymphocytes during immunogenesis. The lectin isolated from Y. pestis EV outer membrane may be regarded as an additional factor ensuring the contact of the pathogen with the cells of the body and as a promising component of combined plague vaccine.
Tikhomirova
E I
EI
Chernyshevsky State University, Research Institute for Plague Control Microbe, Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov, Russia.
Zadnova
S P
SP
Volokh
O A
OA
Karpunina
L V
LV
Shcherbakov
A A
AA
D042842:11-beta-Hydroxysteroid Dehydrogenases; D000279:Administration, Cutaneous; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology; D002845:Chromatography; D006023:Glycoproteins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D006168:Guinea Pigs; D006388:Hemagglutinins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D006913:Hydroxysteroid Dehydrogenases / Q000032:analysis; D007114:Immunization; D037102:Lectins / Q000737:chemistry / Q000276:immunology*; D008213:Lymphocyte Activation; D008970:Molecular Weight; D010930:Plague / Q000097:blood / Q000201:enzymology / Q000276:immunology*; D010931:Plague Vaccine / Q000737:chemistry*; D013045:Species Specificity; D015010:Yersinia pestis / Q000276:immunology*
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D006023:Glycoproteins; D006388:Hemagglutinins; D037102:Lectins; D010931:Plague Vaccine; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases