title: Ornithine decarboxylase promotes catalysis by binding the carboxylate in a buried pocket containing phenylalanine 397.
abstract: Ornithine decarboxylase (ODC) is a pyridoxal 5'-phosphate (PLP) dependent enzyme that catalyzes the decarboxylation of l-Orn to putrescine, a rate-limiting step in the formation of polyamines. The X-ray crystal structures of ODC, complexed to several ligands, support a model where the substrate is oriented with the carboxyl-leaving group buried on the re face of the PLP cofactor. This binding site is composed of hydrophobic and electron-rich residues, in which Phe-397 is predicted to form a close contact. Mutation of Phe-397 to Ala reduces the steady-state rate of product formation by 150-fold. Moreover, single turnover analysis demonstrates that the rate of the decarboxylation step is decreased by 2100-fold, causing this step to replace product release as the rate-limiting step in the mutant enzyme. These data support the structural prediction that the carboxyl-leaving group is positioned to interact with Phe-397. Multiwavelength stopped-flow analysis of reaction intermediates suggests that a major product of the reaction with the mutant enzyme is pyridoximine 5'-phosphate (PMP), resulting from incorrect protonation of the decarboxylated intermediate at the C4' position. This finding was confirmed by HPLC analysis of the reaction products, demonstrating that Phe-397 also plays a role in maintaining the integrity of the reaction chemistry. The finding that the carboxylate-leaving group is oriented on the buried side of the PLP cofactor suggests that ODC facilitates decarboxylation by destabilizing the charged substrate carboxyl group in favor of an electrostatically more neutral transition state.
authors.affiliation: Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9041, USA.
authors.forename: Laurie K
authors.initials: LK
authors.lastname: Jackson
authors.forename: Harold B
authors.initials: HB
authors.lastname: Brooks
authors.forename: David P
authors.initials: DP
authors.lastname: Myers
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Phillips
mesh_terms: D000409:Alanine / Q000235:genetics; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002250:Carbon Radioisotopes; D002264:Carboxylic Acids / Q000737:chemistry* / Q000378:metabolism; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D009952:Ornithine / Q000737:chemistry / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000737:chemistry* / Q000378:metabolism; D010649:Phenylalanine / Q000737:chemistry* / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D013057:Spectrum Analysis; D055672:Static Electricity; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000235:genetics
chemical_list: D002250:Carbon Radioisotopes; D002264:Carboxylic Acids; D010649:Phenylalanine; D009952:Ornithine; D009955:Ornithine Decarboxylase; D000409:Alanine