The use of NASBA for the detection of microbial pathogens in food and environmental samples.
The isothermal amplification method nucleic acid sequence-based amplification (NASBA), which amplifies RNA, has been reported as useful for the detection of microbial pathogens in food and environmental samples. Methods have been published for Campylobacter spp., Listeria monocytogenes and Salmonella enterica ser. Enteritidis in various foods and for Cryptosporidium parvum in water. Both 16S rRNA and various mRNAs have been used as target molecules for detection; the latter may have advantages in allowing specific detection of viable cells. Most of the methods to detect pathogens in foods have employed enrichment in nutrient medium prior to NASBA, as this can ensure sensitivity of detection and encourage the detection of only viable target cells. Although a relatively recent method, NASBA has the potential for adoption as a diagnostic tool for environmental pathogens.
Cook
Nigel
N
DEFRA Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK. n.cook@csl.gov.uk
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification* / Q000472:pathogenicity; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000032:analysis; D004783:Environmental Microbiology; D005516:Food Microbiology; D030041:Hepatitis A virus / Q000302:isolation & purification*; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012401:Rotavirus / Q000302:isolation & purification* / Q000472:pathogenicity; D021161:Self-Sustained Sequence Replication / Q000379:methods*
D004275:DNA, Ribosomal; D012333:RNA, Messenger; D012336:RNA, Ribosomal, 16S