[Diagnosis of imported malaria with multiplex PCR on LighCycler apparatus].
For the diagnosis of imported malaria, a competitive multiplex PCR using LightCycler was developed and compared for 3 months with a traditional PCR method. Hundred eighty three patients were tested by these 2 techniques of molecular diagnosis: 60 were positive for P. falciparum, 9 were positive for other Plasmodium species and 114 were negative. The LightCycler method was found in total agreement with the traditional PCR.
Fabre
R
R
Service de Parasitologie, CHU Rangueil, 31403 4, Toulouse, France.
Berry
A
A
Magnaval
J F
JF
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis; D006801:Humans; D008288:Malaria / Q000175:diagnosis*; D010961:Plasmodium / Q000235:genetics; D010963:Plasmodium falciparum / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation*
D016054:DNA, Protozoan