In vitro culture of human bone marrow mesenchymal stem cell clonies and induced differentiation into neuron-like cells.
OBJECTIVE
To study the long-term in vitro culture of human bone marrow mesenchymal stem cells (hMSC) and their phenotypical and functional properties.


METHODS
Adherent hMSC colonies were digested by 0.25% trypsin-EDTA with a clone cycle for in vitro subculture. Flow cytometry was employed to examine the phenotypes of the cells. Their committed differentiation potential to neurons, clone-forming ability and growth curves were all investigated.


RESULTS
hMSCs could be subcultured under this culture condition for 20 passages, expressing CD13, CD29 and CD59 but not CD11, CD14, CD31, CD34, CD45, CD80, CD86, CD117 and HLA-DR. The cells could be induced to differentiate into neurons when subcultured for 17 passages.


CONCLUSIONS
hMSCs can be efficiently expanded under this culture condition, and the colony-derived hMSCs can maintain the differentiation potentials and retain their biological characteristics.
Lin
Jing-ren
JR
Department of Hematology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Guo
Kun-yuan
KY
Li
Jiang-qi
JQ
Yan
Ding-an
DA
D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D009474:Neurons / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology