[Effects of chrysotile asbestos on the activities of cytochrome P4501A1 and glutathione S-transferase in A549 cell line].
OBJECTIVE
To study the effects of chrysotile on the activities of some enzymes for xenobiotics metabolism.


METHODS
UICC chrysotile (UC) and China Mangya chrysotile (MC) asbestos fibers were used at different doses (0, 25, 50, 100, 200 mg/L) to study its effects on the activities of cytochrome P4501A1 and glutathione S-transferase (GST) in A549 cell line. Also, the effects of chrysotile on the activities of CYP1A1 and GST induced by benzo(a)pyrene were studied.


RESULTS
The activity of EROD increased slowly with the increasing dose of UC, as A549 cells were incubated with UC for 24 h, and EROD activity increased by 40% at a dose of 200 mg/L UC. However, activity of EROD decreased by 32% with 48 h incubation at the same dose, indicating that lower dose of UC and short time could induce the activity of EROD in A549 cells, whereas higher doses and long time could inhibit its activity. MC exhibited a multiphasic effects on the activity of EROD, whether at a dose of 25 mg/L for 24 h or 48 h all gave the strongest induction, 1.86 times or 1.28 times as controls, respectively. However, EROD activity decreased with the increases in MC doses and incubation time, with the lowest as 35% of the controls. The effect of UC on GST activity was not so obvious, with the highest as the increase in its activity by 20%. The highest induction of MC to GST activity was at a dose of 25 mg/L, 2, 5 times as that in controls. With the increases in its doses, effects of MC on GTS activity became inhibition from induction, like that on EROD activity. MC at a dose of 200 mg/L could lower the activity of GST by 18.7%. A549 cells were incubated with chrysotile fiber for 24 h firstly, and then incubated with benzo(a)pyrene to induce the activities of EROD and GST. The results showed that neither UC nor MC could affect the activity of EROD induced by benzo(a)pyrene. However, UC at a dose of 200 mg/L and MC at 100 mg/L could increase the activity of GST induced by benzo(a)pyrene.


CONCLUSIONS
Chrysotile at different doses or its different types showed varied effects on the activities of EROD and GST in A549 cell lines, probably because of different physicochemical characteristics and surface activity of two kinds of asbestos.
Wang
Qi'en
Q
Department of Occupational and Environmental Health, School of Public Health, Peking University, Beijing 100083, China.
Yang
Xinyu
X
Yan
Lei
L
Zhao
Jinghui
J
Liu
Shijie
S
Shen
Huiqi
H
D017632:Asbestos, Serpentine / Q000633:toxicity*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology*; D014407:Tumor Cells, Cultured
D017632:Asbestos, Serpentine; D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase