Peritoneal leukocyte survival and respiratory burst responses in patients treated with a low glucose degradation and high pH peritoneal dialysis fluid.
The discovery of toxicity related to glucose degradation products (GDP) has initiated the development of new PD fluids with low GDP concentrations and higher, more physiological, pH levels. Cell numbers, differential counts and the respiratory burst responses of peritoneal leukocytes were compared between patients treated with the low GDP, high pH fluid Gambrosol-trio (n=10) and a conventional fluid (n=12). Effluents from over-night dwells were collected and leukocytes were evaluated morphologically and by luminol-amplified chemiluminescence (CL) after stimulation with opsonized zymosan. The frequency of necrosis and early apoptosis was quantified by means of annexin V binding and propidium iodide uptake. The Gambrosol-trio group produced significantly higher (p<5%) macrophage counts and stronger CL responses (p<10%) than did the conventional fluid group. The cell compositions did not differ significantly between the groups. Necrosis was significantly more common among the cells in the conventional fluid group. The occurrence of apoptosis did not differ between the fluids.
Jonasson
P
P
Department of Anatomy and Cell Biology, Göteborg University, Sweden.
Albrektsson
A
A
Ljungman
S
S
Wieslander
A
A
Braide
M
M
D000328:Adult; D000368:Aged; D017209:Apoptosis; D002470:Cell Survival / Q000187:drug effects; D015314:Dialysis Solutions / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007674:Kidney Diseases / Q000628:therapy; D007962:Leukocytes / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D008962:Models, Theoretical; D009336:Necrosis; D010530:Peritoneal Dialysis / Q000379:methods*; D016897:Respiratory Burst / Q000187:drug effects*
D015314:Dialysis Solutions; D005947:Glucose