title: Avian reovirus induces an inhibitory effect on lymphoproliferation in chickens.
abstract: The cellular immune responses of chickens inoculated with the vaccine strain S-1133 and/or a field isolate VA-1 of avian reovirus (ARV) were studied. Both strains of virus caused inhibition of the phytohaemagglutinin (PHA)-induced lymphoproliferative response of peripheral blood mononuclear cells (PBMC) and splenic mononuclear cells (SMC) during the initial stage from day 4 up to day 10 post-inoculation (PI), with a later return to the normal value. The inhibition in the PHA-induced lymphoproliferation of SMC could be partially overcome by depletion of adherent cells. The supernatant of the PHA-stimulated SMC culture was also checked in vitro for the presence of suppressive factor(s) produced in response to ARV infection. The culture supernatant from chickens at day 5 PI caused significant inhibition of the PHA-induced lymphoproliferation of control birds, suggesting the presence of suppressive factor(s). ARV infection also significantly inhibited IL-2 production on day 5. There was a significant increase in nitric oxide production by the splenic mononuclear cells of chickens inoculated with either strain of ARV.
authors.affiliation: Immunology Section, Indian Veterinary Research Institute, Izatnagar 243 122 (UP), India.
authors.forename: S
authors.initials: S
authors.lastname: Neelima
authors.forename: G C
authors.initials: GC
authors.lastname: Ram
authors.forename: J M
authors.initials: JM
authors.lastname: Kataria
authors.forename: T K
authors.initials: TK
authors.lastname: Goswami
mesh_terms: D000818:Animals; D002645:Chickens; D017077:Culture Media, Conditioned / Q000378:metabolism; D005260:Female; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D009000:Monocytes / Q000276:immunology; D009573:Nitrites / Q000378:metabolism; D030101:Orthoreovirus, Avian / Q000276:immunology*; D010835:Phytohemagglutinins / Q000276:immunology; D011201:Poultry Diseases / Q000276:immunology / Q000821:virology*; D011897:Random Allocation; D012088:Reoviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology
chemical_list: D017077:Culture Media, Conditioned; D007376:Interleukin-2; D009573:Nitrites; D010835:Phytohemagglutinins