title: mRNA display: ligand discovery, interaction analysis and beyond.
abstract: In vitro peptide and protein selection using mRNA display enables the discovery and directed evolution of new molecules from combinatorial libraries. These selected molecules can serve as tools to control and understand biological processes, enhance our understanding of molecular interactions and potentially treat disease in therapeutic applications. In mRNA display, mRNA molecules are covalently attached to the peptide or protein they encode. These mRNA-protein fusions enable in vitro selection of peptide and protein libraries of >10(13) different sequences. mRNA display has been used to discover novel peptide and protein ligands for RNA, small molecules and proteins, as well as to define cellular interaction partners of proteins and drugs. In addition, several unique applications are possible with mRNA display, including self-assembling protein chips and library construction with unnatural amino acids and chemically modified peptides.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Pasadena, California 91125, USA.
authors.forename: Terry T
authors.initials: TT
authors.lastname: Takahashi
authors.forename: Ryan J
authors.initials: RJ
authors.lastname: Austin
authors.forename: Richard W
authors.initials: RW
authors.lastname: Roberts
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000939:Epitopes / Q000378:metabolism; D020869:Gene Expression Profiling; D008024:Ligands; D008954:Models, Biological; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000378:metabolism; D011487:Protein Conformation; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D019809:Streptavidin / Q000378:metabolism
chemical_list: D000939:Epitopes; D008024:Ligands; D010455:Peptides; D012313:RNA; D000255:Adenosine Triphosphate; D019809:Streptavidin