[The application of dual fusion interphase fluorescence in situ hybridization probe in detecting minimal residual disease in chronic myeloid leukemia].
OBJECTIVE
To explore the sensitivity, specificity and reproducibility of dual-color and dual-fusion fluorescence in situ hybridization (D-FISH) in detecting the minimal residual disease (MRD) of chronic myeloid leukemia (CML) after treatment.


METHODS
MRD were detected in the bone marrow of 8 cases of chronic myeloid leukemia after treatment with interphase fluorescence in situ hybridization. Sensitivity, specificity and reproducibility of D-FISH were compared with those of single-fusion FISH (S-FISH). Furthermore, correlation between the results of D-FISH and RT-PCR was explored.


RESULTS
D-FISH was more sensitive and specific than S-FISH and had a good reproducibility. In normal control, the cutoff rates of D-FISH and S-FISH were 0.570% and 5.868% with significant statistic difference. In detection of MRD in 8 cases of CML post transplantation, positive rates of S-FISH and D-FISH were 3/8 (37.5%) and 5/8 (62.5%) respectively. Furthermore, the results of D-FISH had a high correlation with that of RT-PCR.


CONCLUSIONS
In view of the lower false-positive and false-negative results of D-FISH, it may be used in the detection of minimal residual disease in CML after treatment. But further exploration is required for verifying the correlation between the results of D-FISH and the clinical parameters.
Du
Qingfeng
Q
Department of Hematology, Nanfang Hospital, The First Military Medical University, Guangzhou 510515, China. dupontping@sohu.com
Liu
Xiaoli
X
Song
Lanlin
L
Meng
Fanyi
F
Zhou
Shouyun
S
D000293:Adolescent; D000328:Adult; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D018365:Neoplasm, Residual / Q000175:diagnosis*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D033581:Stem Cell Transplantation