title: DNA sequences in cationic lipid:pDNA-mediated systemic toxicities.
abstract: Systemic delivery of synthetic gene transfer vectors such as cationic lipid:plasmid DNA (pDNA) complexes elicits a range of acute physiologic responses, which in the context of therapeutic gene delivery represent dose-limiting toxicities. The most prominent responses are transient leukopenia, thrombocytopenia, serum transaminase elevations, and elevations of proinflammatory cytokines such as interferon-gamma (IFN-gamma), interleukin-12 (IL-12), and tumor necrosis factor-alpha (TNF-alpha). The unmethylated CpG sequences present in plasmid DNA have been implicated as a major cause of the robust cytokine response that follows systemic administration of cationic lipid:pDNA complexes. However, the factors causing the additional significant toxicities (leukopenia, thrombocytopenia, and serum transaminase elevations) recently shown to be associated with vector administration have not been defined. We show here that DNA sequences, such as immune stimulatory CpG sequences, play a significant role in inducing the additional acute toxicities associated with cationic lipid:pDNA complex administration. Importantly, while methylating these CpG sequences results in greatly reduced cytokine levels, this modification does not eliminate their ability to generate the other systemic toxicities. Examples of non-CpG DNA sequences that induce distinct toxicity profiles when administered systemically in the form of cationic lipid:DNA complexes are also identified. Taken together, these results imply that specific DNA sequences are responsible for a significant portion of the systemic toxicities observed after administration of cationic lipid:pDNA complexes.
authors.affiliation: Genzyme Corporation, Framingham, MA 01701-9322, USA.
authors.forename: Jennifer D
authors.initials: JD
authors.lastname: Tousignant
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhao
authors.forename: Nelson S
authors.initials: NS
authors.lastname: Yew
authors.forename: Seng H
authors.initials: SH
authors.lastname: Cheng
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Eastman
authors.forename: Ronald K
authors.initials: RK
authors.lastname: Scheule
mesh_terms: D000818:Animals; D018899:CpG Islands; D016207:Cytokines / Q000378:metabolism; D004247:DNA / Q000008:administration & dosage / Q000378:metabolism* / Q000633:toxicity; D019175:DNA Methylation; D018664:Interleukin-12 / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000008:administration & dosage / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D016207:Cytokines; D008055:Lipids; D018664:Interleukin-12; D004247:DNA