title: Sympathetic mediated vasomotion and skin capillary permeability in diabetic patients with peripheral neuropathy.
abstract: OBJECTIVE
  A loss of sympathetic function could lead to changes in capillary fluid filtration in diabetic patients. We investigated whether a decreased sympathetically mediated vasomotion in the skin in diabetic patients with peripheral neuropathy is associated with an abnormal capillary leakage.
  
  
  METHODS
  Three matched groups were studied: 18 diabetic patients with documented peripheral neuropathy (DN), 18 diabetic patients without peripheral neuropathy (D), and 18 healthy control subjects (C). Sensory and motor nerve function of the distal extremities were assessed by standard neurography, and expressed in a sensory-motor nerve function score. Sympathetic vasomotion of the skin microcirculation was assessed by determining the power of blood flow variability in the low-frequency (0.02-0.14 Hz) band by spectral analysis of laser Doppler flowmetry at the median ankle. Skin capillary leakage was evaluated by sodium fluorescein videodensitometry at the same site of the foot.
  
  
  RESULTS
  Sympathetically mediated vasomotion of the foot skin microcirculation was lower in diabetic patients with documented peripheral neuropathy compared with diabetic patients without peripheral neuropathy and control subjects (p<0.001). Capillary sodium fluorescein leakage was larger in 18 diabetic patients with documented peripheral neuropathy than in diabetic patients without peripheral neuropathy (p<0.02) and C (p<0.005). Multiple regression analysis disclosed that a reduced sympathetically mediated vasomotion, together with a lower sensory-motor nerve function score, independently contributed to the variance in sodium fluorescein leakage, for 30% (p<0.001) and 17% (p<0.01), respectively.
  
  
  CONCLUSIONS
  A loss of sympathetic tone, apart from sensory-motor nerve dysfunction, seems to be a major determinant of an increased capillary permeability in diabetic patients with neuropathy.
authors.affiliation: Division of Angiology, Department of Internal Medicine, University Hospital, Groningen, The Netherlands.
authors.forename: J D
authors.initials: JD
authors.lastname: Lefrandt
authors.forename: E
authors.initials: E
authors.lastname: Bosma
authors.forename: P H N
authors.initials: PH
authors.lastname: Oomen
authors.forename: J H
authors.initials: JH
authors.lastname: Hoeven
authors.forename: A M
authors.initials: AM
authors.lastname: Roon
authors.forename: A J
authors.initials: AJ
authors.lastname: Smit
authors.forename: K
authors.initials: K
authors.lastname: Hoogenberg
mesh_terms: D002199:Capillary Permeability; D016022:Case-Control Studies; D003287:Contrast Media / Q000493:pharmacokinetics; D003922:Diabetes Mellitus, Type 1 / Q000150:complications; D003924:Diabetes Mellitus, Type 2 / Q000150:complications; D003929:Diabetic Neuropathies / Q000209:etiology / Q000503:physiopathology*; D005260:Female; D019793:Fluorescein / Q000493:pharmacokinetics; D005528:Foot; D006801:Humans; D017078:Laser-Doppler Flowmetry; D008297:Male; D008875:Middle Aged; D010523:Peripheral Nervous System Diseases / Q000209:etiology / Q000503:physiopathology*; D012039:Regional Blood Flow; D012867:Skin / Q000098:blood supply*; D013564:Sympathetic Nervous System / Q000503:physiopathology*; D014666:Vasomotor System / Q000503:physiopathology*
chemical_list: D003287:Contrast Media; D019793:Fluorescein