Human T-cell leukemia virus type 1 Tax protein stimulates the interferon-responsive enhancer element via NF-kappaB activity.
Gene expression in Rat-1 fibroblast cells transformed by Tax from human T-cell leukemia virus type 1 was studied using the reverse transcriptase polymerase chain reaction differential display technique. The analysis revealed eight genes that were upregulated and one gene that was suppressed in Tax-transformed cells. Interestingly, at least four of the upregulated genes were interferon-stimulated genes. Promoter analysis of the 2',5'-oligoadenylate synthetase gene, which was activated in both Tax-transformed Rat-1 cells and primary adult T-cell leukemia cells, demonstrated that Tax indirectly activates its interferon-responsive enhancer element in a nuclear factor-kappaB pathway-dependent manner, indicating a close association of interferon signaling with the transformation by Tax.
Shimizu
Toshiki
T
Department of Microbiology, Kansai Medical University, Fumizono-cho 10-15, Moriguchi, Osaka, Japan.
Kawakita
Shigenari
S
Li
Qin-Hua
QH
Fukuhara
Shirou
S
Fujisawa
Jun-ichi
J
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics; D000818:Animals; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D054357:Chemokine CXCL10; D019743:Chemokines, CXC / Q000494:pharmacology; D004742:Enhancer Elements, Genetic; D015967:Gene Expression Regulation, Viral; D016356:Gene Products, tax / Q000502:physiology*; D006367:HeLa Cells; D015368:Human T-lymphotropic virus 1 / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000097:blood; D008214:Lymphocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
D054357:Chemokine CXCL10; D019743:Chemokines, CXC; D016356:Gene Products, tax; D016328:NF-kappa B; D007371:Interferon-gamma; D015088:2',5'-Oligoadenylate Synthetase