Primary African HIV clade A and D isolates: effective cross-clade neutralization with a quadruple combination of human monoclonal antibodies raised against clade B.
We investigated the ability of several human neutralizing monoclonal antibodies (nmAbs), originally raised against human immunodeficiency virus (HIV) clade B isolates, to neutralize primary clade A and D isolates as single agents and in combinations. All four primary HIV clade A isolates and five primary HIV clade D isolates tested were neutralized >99% by the quadruple combination of nmAbs IgG1b12, 2G12, 2F5, and 4E10. These mAbs recognize conserved epitopes on HIV-1 envelope (Env), resulting in strong cross-clade neutralization. Previously, we showed synergistic neutralization of primary HIV-1 clade C isolates in vitro by the same nMAb combination. We and others also showed neutralization of primary HIV clade B strains. Together, our data show that the quadruple combination of mAbs effectively neutralized primary HIV clade A, B, C, and D isolates.
Kitabwalla
Moiz
M
Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
Ferrantelli
Flavia
F
Wang
Tao
T
Chalmers
Alistair
A
Katinger
Hermann
H
Stiegler
Gabriela
G
Cavacini
Lisa A
LA
Chou
Ting-Chao
TC
Ruprecht
Ruth M
RM
D000349:Africa; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions; D015483:HIV Antibodies / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D009500:Neutralization Tests
D000911:Antibodies, Monoclonal; D015483:HIV Antibodies