A jasmonate-responsive element within the A. thaliana vsp1 promoter.
The vsp1 gene of Arabidopsis thaliana encodes a storage protein that accumulates in vegetative organs. Transgenic plants expressing a vsp1 promoter-gus (beta-glucuronidase) gene fusion were found to contain high GUS activity when challenged with jasmonate, a volatile plant hormone. The induction of vsp1-gus expression by jasmonate could be measured in tobacco mesophyll protoplasts, after transient expression. A number of deletions were operated in the vsp1 promoter in order to locate its jasmonate-responsive element. A 41 bp sequence taken approximately 150 bp upstream of the vsp1 TATA box could confer jasmonate responsiveness upon a short CaMV 35S promoter. Whereas the deletion of a CAAT box-like element within the 41 bp sequence did not affect promoter activity, mutation of a short palindromic motif completely abolished jasmonate responsiveness. This motif shows no homology with the jasmonate-responsive elements of other promoters.
Guerineau
F
F
Université de Picardie Jules Verne, 33 rue St Leu, F-80039 Amiens, France. francois.guerineau@sc.u-picardie.fr
Benjdia
M
M
Zhou
D X
DX
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D003517:Cyclopentanes / Q000494:pharmacology*; D010450:Endopeptidases / Q000235:genetics*; D017353:Gene Deletion; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D054883:Oxylipins; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011523:Protoplasts / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016385:TATA Box / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D011993:Recombinant Fusion Proteins; C011006:jasmonic acid; C441479:VSP1 protein, Arabidopsis; D005966:Glucuronidase; D010450:Endopeptidases