title: [Function analysis of a wheat phosphate transporter in yeast mutant].
abstract: Phosphorus is a major nutrient acquired by plants and phosphorus availability is considered one of the major growth-limiting factors for plants in many natural ecosystems. For a better understanding of the function of the wheat phosphorus transporter gene named TaPT2, we did Southern blot analysis and studied its function with complementation test in yeast mutant strain MB192. Southern blot indicated that TaPT2 gene is a low-copy member and has several different members in wheat genome. In the function complementation study, TaPT2 has a similar function as PHO84 which is the phosphate transporter in yeast Saccharomyces cerevisiae. Both TaPT2 and PHO84 are able to complement the PHO84 mutant phenotype of yeast strain MB192 which lacks the phosphate transporter activity. Therefor, this assessment presents evidence that TaPT2 gene plays an important role in Pi acquisition.
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Ya-Juan
authors.initials: YJ
authors.lastname: Zeng
authors.forename: Jia
authors.initials: J
authors.lastname: Ying
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Liu
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Sun
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Hua-Shan
authors.initials: HS
authors.lastname: Xiao
authors.forename: Zhen-Sheng
authors.initials: ZS
authors.lastname: Li
mesh_terms: D015139:Blotting, Southern; D002455:Cell Division / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005816:Genetic Complementation Test; D009154:Mutation; D028061:Phosphate Transport Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
chemical_list: D018744:DNA, Plant; D028061:Phosphate Transport Proteins