title: Cloning of a novel one-repeat calcium channel-like gene.
abstract: We describe the cloning of a cDNA from a human testis library that encodes a novel protein with similarity to one repeat of voltage-gated Ca(2+) channels (Ca(v)). Northern and dot blot analyses indicate that the novel Ca(v)-like gene is expressed predominantly in testis and at lower levels in many other tissues. Heterologous expression of the Ca(v)-like protein did not lead to the induction of any detectable ionic current and failed to modify intracellular Ca(2+) concentrations. Similar one-repeat Ca(v)-like proteins have been cloned from Bacillus, Mus, and Homo, and appear to encode ion channels involved in renal function, axis determination, and sperm motility.
authors.affiliation: Department of Pharmacology, University of Virginia, P.O. Box 800735, 1300 Jefferson Park Avenue, Charlottesville 22908-0735, VA, USA.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Arias
authors.forename: Janet
authors.initials: J
authors.lastname: Murbartián
authors.forename: Edward
authors.initials: E
authors.lastname: Perez-Reyes
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D004594:Electrophysiology; D005091:Exons; D005260:Female; D015723:Gene Library; D006801:Humans; D007438:Introns; D007473:Ion Channels; D007477:Ions; D008297:Male; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009865:Oocytes; D011485:Protein Binding; D012313:RNA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism; D014018:Tissue Distribution; D014982:Xenopus laevis
chemical_list: D015220:Calcium Channels; D007473:Ion Channels; D007477:Ions; D012313:RNA; D002118:Calcium