title: BCL-2/JH translocation in peripheral blood lymphocytes of unexposed individuals: lack of seasonal variations in frequency and molecular features.
abstract: BCL-2/J(H) rearrangement has been proposed as a biomarker for evaluating the genotoxicity of occupational and environmental exposures. Available data on time-related modification of this rearrangement in peripheral blood lymphocytes in unexposed healthy individuals is scarce. We investigated the characteristics of BCL-2/J(H) rearrangements in 33 adults unexposed to genotoxins at 2 seasonal time points: winter and spring. BCL-2/J(H) rearrangement was detected in 79% of individuals (detection limit = 8.48 x 10(-8)). Its frequency ranged from <1 to 40 translocations per million lymphocytes with a significant (p = 0.04) positive correlation with age. No significant modifications of BCL-2/J(H) rearrangement frequency or in the number of clones harboring this rearrangement were observed according the 2 time points. No obvious influence of season-related environmental factors on frequency or molecular features of BCL-2/J(H) rearrangements was found in this population suggesting that this would not be a confounding factor.
authors.affiliation: Laboratoire de Cancérologie Expérimentale, Centre François Baclesse, Caen, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Roulland
authors.forename: Pierre
authors.initials: P
authors.lastname: Lebailly
authors.forename: Gilbert
authors.initials: G
authors.lastname: Roussel
authors.forename: Mélanie
authors.initials: M
authors.lastname: Briand
authors.forename: David
authors.initials: D
authors.lastname: Cappellen
authors.forename: Didier
authors.initials: D
authors.lastname: Pottier
authors.forename: Agnès
authors.initials: A
authors.lastname: Hardouin
authors.forename: Xavier
authors.initials: X
authors.lastname: Troussard
authors.forename: Christian
authors.initials: C
authors.lastname: Bastard
authors.forename: Michel
authors.initials: M
authors.lastname: Henry-Amar
authors.forename: Pascal
authors.initials: P
authors.lastname: Gauduchon
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D004247:DNA / Q000032:analysis; D017931:DNA Primers; D005434:Flow Cytometry; D005787:Gene Frequency; D015321:Gene Rearrangement; D006801:Humans; D007133:Immunoglobulin Joining Region / Q000235:genetics*; D008214:Lymphocytes / Q000097:blood*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D012621:Seasons; D014178:Translocation, Genetic / Q000235:genetics*
chemical_list: D017931:DNA Primers; D007133:Immunoglobulin Joining Region; D019253:Proto-Oncogene Proteins c-bcl-2; D004247:DNA