In vivo studies of liver-type fatty acid binding protein (L-FABP) gene expression in liver of transgenic zebrafish (Danio rerio).
Mammalian liver fatty acid binding protein (L-FABP) is a small cytosolic protein in various tissues including liver, small intestine and kidney and is thought to play a crucial role in intracellular fatty acid trafficking and metabolism. To better understand its tissue-specific regulation during zebrafish hepatogenesis, we isolated 5'-flanking sequences of the zebrafish L-FABP gene and used a green fluorescent protein (GFP) transgenic strategy to generate liver-specific transgenic zebrafish. The 2.8-kb 5'-flanking sequence of zebrafish L-FABP gene was sufficient to direct GFP expression in liver primordia, first observed in 2 dpf embryos and then continuously to the adult stage. This pattern of transgenic expression is consistent with the expression pattern of the endogenous gene. F2 inheritance rates of 42-51% in all the seven transgenic lines were consistent with the ratio of Mendelian segregation. Further, hhex and zXbp-1 morphants displayed a visible liver defect, which suggests that it is possible to establish an in vivo system for screening genes required for liver development.
Her
Guor Mour
GM
Laboratory of Marine Molecular Biology and Biotechnology, Institute of Zoology, Academia Sinica, Nankang, Taipei 115, Taiwan.
Chiang
Chia-Chang
CC
Chen
Wen-Ya
WY
Wu
Jen-Leih
JL
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D049452:Green Fluorescent Proteins; D008099:Liver / Q000378:metabolism*; D008164:Luminescent Proteins / Q000378:metabolism; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D016133:Polymerase Chain Reaction; D015027:Zebrafish; D029961:Zebrafish Proteins
D002352:Carrier Proteins; D017931:DNA Primers; C493320:Fabp1 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D008164:Luminescent Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D029961:Zebrafish Proteins; C490175:fabp7a protein, zebrafish; D049452:Green Fluorescent Proteins