[Standardization of neutralization tests using the COBL cell line and comparison with the particle agglutination test for measles serology].
The aim of the present study was the detection and comparison of measles antibody titers with particle agglutination (PA) and neutralization (Nt) methods, in the sera samples of 364 subjects from different age groups. PA method was performed with a commercial test kit (Serodiameasles, Fujirebio Com. Japan), and Nt test which was standardized in this study, by using COBL (cord blood) cell lines, has been started to use in our laboratory as a reference method. As a result, antibody titers detected by PA were in parallel to the titers which detected by Nt test, and it was concluded that the differences in antibody titers would arise from the differences of test principles and viral antigens.
Korukluoğlu
Gülay
G
Refik Saydam Hifzissihha Merkezi, Viroloji Laboratuvari, Ankara.
Yalçinkaya
Tülay
T
Ozkaya
Etem
E
Kurtoğlu
Demet
D
Gözalan
Ayşegül
A
Miyamura
Kikuko
K
D000293:Adolescent; D000328:Adult; D000372:Agglutination Tests / Q000592:standards*; D000914:Antibodies, Viral / Q000097:blood*; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008457:Measles / Q000175:diagnosis* / Q000276:immunology; D008459:Measles virus / Q000276:immunology*; D009500:Neutralization Tests / Q000592:standards*; D011933:Reagent Kits, Diagnostic
D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic