Establishment of an enzyme-linked immunosorbent assay for detection of hantavirus antibody of rats using a recombinant of nucleocapsid protein expressed in Escherichia coli.
A recombinant nucleocapsid protein of Hantaan virus (HTN) 76-118 strain expressed in E. coli was applied as a serodiagnostic antigen in an enzyme-linked immunosorbent assay (rHTN-ELISA) for detection of hantavirus antibody in rat sera. The sensitivity and specificity of the rHTN-ELISA were compared with those of the indirect immunofluoresent assay (IFA) using virus-infected cells. The sensitivity of rHTN-ELISA was similar to that of the IFA both in experimentally SR-11 infected rat and naturally infected rat sera. Sera showing a low antibody titer in IFA and suspected to be negative by other methods were also found to be negative in rHTN-ELISA. These results indicate that rHTN-ELISA is effective as a screening method for serodiagnosis of hantaviruses, because of its high sensitivity, specificity, safety and suitability for processing large number of samples.
Takakura
Akira
A
Central Institute for Experimental Animals, 1430 Nogawa, Miyamae, Kawasaki, 216-0001, Japan.
Goto
Kazuo
K
Itoh
Toshio
T
Yoshimatsu
Kumiko
K
Takashima
Ikuo
I
Arikawa
Jiro
J
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001426:Bacterial Proteins; D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004926:Escherichia coli; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006476:Orthohantavirus / Q000276:immunology*; D018778:Hantavirus Infections / Q000175:diagnosis*; D019590:Nucleocapsid Proteins; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*
D000914:Antibodies, Viral; D001426:Bacterial Proteins; D015415:Biomarkers; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins