Rapid direct sequence analysis of the dystrophin gene.
Mutations in the dystrophin gene result in both Duchenne and Becker muscular dystrophy (DMD and BMD), as well as X-linked dilated cardiomyopathy. Mutational analysis is complicated by the large size of the gene, which consists of 79 exons and 8 promoters spread over 2.2 million base pairs of genomic DNA. Deletions of one or more exons account for 55%-65% of cases of DMD and BMD, and a multiplex polymerase chain reaction method-currently the most widely available method of mutational analysis-detects approximately 98% of deletions. Detection of point mutations and small subexonic rearrangements has remained challenging. We report the development of a method that allows direct sequence analysis of the dystrophin gene in a rapid, accurate, and economical fashion. This same method, termed "SCAIP" (single condition amplification/internal primer) sequencing, is applicable to other genes and should allow the development of widely available assays for any number of large, multiexon genes.
Flanigan
Kevin M
KM
Department of Neurology, University of Utah, Salt Lake City, UT, USA. kevin.flanigan@genetics.utah.edu
von Niederhausern
Andrew
A
Dunn
Diane M
DM
Alder
Jonathan
J
Mendell
Jerry R
JR
Weiss
Robert B
RB
D001483:Base Sequence; D002311:Cardiomyopathy, Dilated / Q000235:genetics; D016189:Dystrophin / Q000235:genetics*; D005817:Genetic Counseling; D006801:Humans; D008969:Molecular Sequence Data; D020388:Muscular Dystrophy, Duchenne / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods
D016189:Dystrophin