title: Flow cytometric assessment of ethylene glycol monoethyl ether on spermatogenesis in rats.
abstract: The effects of ethylene glycol monoethyl ether (EGEE) on testicular cell populations in rats were investigated by a flow cytometric method. Rats were administered by gavage with EGEE at the various doses of 0 (saline alone), 100, 200, 400, and 800 mg/kg body weight/day for 4 weeks. The treatment of EGEE caused decreases in the weight of testis and epididymis and in the number of testicular cells. Histopathologically, exfoliation of germ cells into the tubular lumen was observed at the doses of above 200 mg/kg. The treatment of EGEE at the dose of 400 mg/kg caused moderate testicular degeneration. A significant depletion of haploid cells and a disproportionate ratio of diploid and tetraploid cells were observed as determined by flow cytometric analysis. These results indicate that the toxic effect of EGEE on the male reproductive system may be strongly associated with the disproportion of testicular germ cells.
authors.affiliation: Department of Pathology, National Institute of Toxicological Research, Korea Food & Drug Administration, Seoul, Japan.
authors.forename: Chang Yong
authors.initials: CY
authors.lastname: Yoon
authors.forename: Choong Man
authors.initials: CM
authors.lastname: Hong
authors.forename: Yong-Yeon
authors.initials: YY
authors.lastname: Cho
authors.forename: Yong Hyun
authors.initials: YH
authors.lastname: Chung
authors.forename: Hong Ki
authors.initials: HK
authors.lastname: Min
authors.forename: Young Won
authors.initials: YW
authors.lastname: Yun
authors.forename: Beom Jun
authors.initials: BJ
authors.lastname: Lee
authors.forename: Ki Hwa
authors.initials: KH
authors.lastname: Yang
authors.forename: Yong Soon
authors.initials: YS
authors.lastname: Lee
authors.forename: Cheol Kyu
authors.initials: CK
authors.lastname: Kim
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004247:DNA / Q000032:analysis; D005026:Ethylene Glycols / Q000633:toxicity*; D005434:Flow Cytometry; D008297:Male; D011003:Ploidies; D051381:Rats; D017207:Rats, Sprague-Dawley; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*; D013737:Testis / Q000166:cytology / Q000473:pathology
chemical_list: D005026:Ethylene Glycols; D004247:DNA; C021296:2-ethoxyethanol