Accumulation of phosphorylated repressor for gibberellin signaling in an F-box mutant.
Gibberellin (GA) regulates growth and development in plants. We isolated and characterized a rice GA-insensitive dwarf mutant, gid2. The GID2 gene encodes a putative F-box protein, which interacted with the rice Skp1 homolog in a yeast two-hybrid assay. In gid2, a repressor for GA signaling, SLR1, was highly accumulated in a phosphorylated form and GA increased its concentration, whereas SLR1 was rapidly degraded by GA through ubiquitination in the wild type. We conclude that GID2 is a positive regulator of GA signaling and that regulated degradation of SLR1 is initiated through GA-dependent phosphorylation and finalized by an SCF(GID2)-proteasome pathway.
Sasaki
Akie
A
BioScience Center, Nagoya University, Nagoya 464-8601, Japan.
Itoh
Hironori
H
Gomi
Kenji
K
Ueguchi-Tanaka
Miyako
M
Ishiyama
Kanako
K
Kobayashi
Masatomo
M
Jeong
Dong-Hoon
DH
An
Gynheung
G
Kitano
Hidemi
H
Ashikari
Motoyuki
M
Matsuoka
Makoto
M
D000483:Alleles; D000595:Amino Acid Sequence; D018388:Codon, Terminator; D004790:Enzyme Induction; D017343:Genes, Plant; D005875:Gibberellins / Q000378:metabolism* / Q000494:pharmacology; D008025:Ligases / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism; D010641:Phenotype; D010766:Phosphorylation; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D017434:Protein Structure, Tertiary; D012639:Seeds / Q000378:metabolism; D015398:Signal Transduction; D025801:Ubiquitin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases; D000516:alpha-Amylases / Q000096:biosynthesis
D018388:Codon, Terminator; D005875:Gibberellins; D010940:Plant Proteins; D025801:Ubiquitin; C007842:gibberellic acid; D044767:Ubiquitin-Protein Ligases; D000516:alpha-Amylases; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease; D008025:Ligases