title: Interleukin 1beta and tumour necrosis factor alpha secreting cells are increased in the peripheral blood of patients with primary Sjögren's syndrome.
abstract: OBJECTIVE
  To study systemic alterations of cytokine secreting peripheral blood mononuclear cells (PBMC) in primary Sjögren's syndrome (pSS) and their relation to common clinical and immunological manifestations of this disease.
  
  
  METHODS
  PBMC spontaneously secreting tumour necrosis factor alpha (TNFalpha), interleukin 1beta (IL1beta), and interleukin 6 (IL6) were assessed by enzyme linked immunospot (ELISPOT) analysis in a cohort of 31 patients with pSS fulfilling the modified European classification criteria. Nineteen healthy volunteers served as controls. ELISPOT results were correlated with glandular and extraglandular manifestations and autoantibody titres-that is, rheumatoid factor (RF) isotypes, anti-Ro/SS-A, anti-La/SS-B as determined by an enzyme linked immunosorbent assay (ELISA) technique.
  
  
  RESULTS
  The number of TNFalpha and IL1beta secreting cells was significantly higher in patients with pSS than in controls. No differences were detected in the number of IL6 secreting PBMC. Patients with recurrent parotid swelling (RPS) had a significantly increased number of IL1beta secreting PBMC. Moreover, the number of IL1beta secreting PBMC correlated with the disease duration (r(s)=0.479; p<0.01) and with the concentration of IgM RF (r(s)=0.63; p<0.01) and IgG RF (r(s)=0.42; p<0.05). Other autoantibodies did not correlate with cytokine secreting PBMC.
  
  
  CONCLUSIONS
  The increased systemic secretion of IL1beta and TNFalpha in patients with pSS points to a pathogenic impact of these cytokines in this autoimmune disease. In particular the correlation of IL1beta secreting PBMC with RPS and RF production indicates that IL1beta is a crucial regulator in the development of local and systemic disease manifestations.
authors.affiliation: Department of Medicine B, Westphalian Wilhelms-University, Muenster, Germany. willeke.peter@uni-muenster.de
authors.forename: P
authors.initials: P
authors.lastname: Willeke
authors.forename: H
authors.initials: H
authors.lastname: Schotte
authors.forename: B
authors.initials: B
authors.lastname: Schlüter
authors.forename: M
authors.initials: M
authors.lastname: Erren
authors.forename: H
authors.initials: H
authors.lastname: Becker
authors.forename: A
authors.initials: A
authors.lastname: Dyong
authors.forename: E
authors.initials: E
authors.lastname: Mickholz
authors.forename: W
authors.initials: W
authors.lastname: Domschke
authors.forename: M
authors.initials: M
authors.lastname: Gaubitz
mesh_terms: D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012859:Sjogren's Syndrome / Q000097:blood / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha