Ets, Ap-1 and GATA factor families regulate the utrophin B promoter: potential regulatory mechanisms for endothelial-specific expression.
Duchenne muscular dystrophy is caused by dystrophin deficiency, which can be prevented in the mdx mouse model by over-expression of an autosomal homologue, utrophin. Utrophin has two characterised full-length promoters, A and B. No data are available on the transcriptional regulation of B utrophin, which has been recently localised to the endothelium. Similar to characterised endothelial promoters, Ets and Ap-1 individually trans-activate the human B core promoter. Synergistic activation by GATA-2 and c-jun to the order of 20-fold was observed.
Perkins
Kelly J
KJ
Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Davies
Kay E
KE
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000235:genetics*; D024202:Electrophoretic Mobility Shift Assay; D004727:Endothelium / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014157:Transcription Factors / Q000502:physiology*; D015533:Transcriptional Activation / Q000187:drug effects; D049411:Utrophin
D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D014157:Transcription Factors; C483600:Utrn protein, mouse; D049411:Utrophin; D013755:Tetradecanoylphorbol Acetate