Iron particle labeling of haematopoietic progenitor cells: an in vitro study.
We present a method for labeling bone marrow haematopoietic progenitor cells with iron particles. Labeling was assessed by magnetic resonance imaging and electron microscopy. Labeling with iron particles could allow the following by imaging techniques of haematopoietic cells in physiologic and pathologic conditions such as the engraftment of haematopoietic progenitor cells or the migration of myelomonocytic cells in inflammatory diseases.
de Laquintane
Benoit Doche
BD
Laboratoire de Neurobiologie des Affections de la Myéline EA-2966, BP 78, Université Victor Ségalen, 33076 Bordeaux, France.
Dousset
Vincent
V
Solanilla
Anne
A
Petry
Klaus G
KG
Ripoche
Jean
J
D000818:Animals; D002465:Cell Movement / Q000502:physiology; D003911:Dextrans; D052203:Ferrosoferric Oxide; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000648:ultrastructure*; D019044:Image Cytometry / Q000295:instrumentation / Q000379:methods*; D007249:Inflammation / Q000503:physiopathology; D007501:Iron; D008279:Magnetic Resonance Imaging / Q000295:instrumentation / Q000379:methods*; D058185:Magnetite Nanoparticles; D008297:Male; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods*; D010087:Oxides; D051381:Rats; D017207:Rats, Sprague-Dawley
D003911:Dextrans; D058185:Magnetite Nanoparticles; D010087:Oxides; C097921:ferumoxtran-10; D007501:Iron; D052203:Ferrosoferric Oxide