title: Prognostic value of immunocytochemical determination of parathyroid hormone-related peptide expression in cells of mammary ductal carcinoma. Analysis of 7 years of the disease course.
abstract: Parathyroid hormone-related peptide (PTHrP) participates in the development of humoral hypercalcaemia of malignancy. The peptide is thought to affect growth and differentiation of normal and neoplastic cells. The present study aimed at evaluation of the relationship between survival time and development of distant metastases in patients with ductal mammary carcinoma on the one hand and PTHrP expression on the other. Immunocytochemical reactions using mouse monoclonal (clone 212-10.7) anti-PTHrP (38-64) antibodies were performed in paraffin sections originating from 47 patients with ductal mammary carcinoma. Expression of the protein was quantified employing a scale, considering the number of positive cells and intensity of the reaction (immunoreactive score, IRS). Survival time of the patients, determined during the course of a 7-year observation was also analysed. The obtained results demonstrated a relationship between intensity of PTHrP expression and the survival time. Patients with high expression of PTHrP (IRS>6) manifested longer survival than patients with lower PTHrP expression (IRS< or =6; Cox'es F test, P<0.05). Moreover, in the group with the lower PTHrP expression, a negative relationship was detected between expression of the protein and the survival time (Cox'es model, P<0.05). No relationship was detected between PTHrP expression and the development of distant metastases.
authors.affiliation: Department of Histology and Embryology, University School of Medicine, ul.Chałubińskiego 6a, 50-356, Wrocław, Poland. pawel.surowiak@interia.pl
authors.forename: Paweł
authors.initials: P
authors.lastname: Surowiak
authors.forename: Piotr
authors.initials: P
authors.lastname: Dziegiel
authors.forename: Rafał
authors.initials: R
authors.lastname: Matkowski
authors.forename: Mirosław
authors.initials: M
authors.lastname: Sopel
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wojnar
authors.forename: Jan
authors.initials: J
authors.lastname: Kornafel
authors.forename: Maciej
authors.initials: M
authors.lastname: Zabel
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016015:Logistic Models; D008408:Mastectomy; D008875:Middle Aged; D009362:Neoplasm Metastasis; D044162:Parathyroid Hormone-Related Protein; D036361:Peptide Hormones / Q000032:analysis*; D011379:Prognosis; D011878:Radiotherapy; D015996:Survival Rate; D013629:Tamoxifen / Q000627:therapeutic use
chemical_list: D000911:Antibodies, Monoclonal; C470121:PTHLH protein, human; D044162:Parathyroid Hormone-Related Protein; D036361:Peptide Hormones; D013629:Tamoxifen