[A new model of choroidal neovascularization in mice].
OBJECTIVE
To investigate the feasibility of inducing choroidal neovascularization (CNV) in mice by diode laser.


METHODS
Two hours, 3 days, 1 week, 2 weeks and 4 weeks after photocoagulation, the effect of diode laser for inducing CNV by fundus fluorescein angiography (FFA), light microscopy and transmission electron microscopy.


RESULTS
Two hours and 3 days after laser treatment, there were no CNV and fluorescent leakage observed in mice. The first signs of leakage in FFA associated with CNV appeared 1 week after the postphotocoagulation, (48/90 lesions, or 53.3%). Four weeks after laser treatment, there were still 33 lesions appearing leakage (36.67%). Histological sections proved the existence of CNV, but the rate of CNV was not in accord with that of fluorescence leakage.


CONCLUSIONS
This animal model is very useful for in vivo studies of angiogenesis and its modulation via various therapy.
Wang
Kang
K
Ophthalmic Center, the Affiliated Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China. lanbowang1972@yahoo.com
Wang
Kangsun
K
Wang
Ling
L
Chen
Rongjia
R
Shi
Haiyun
H
D000818:Animals; D020256:Choroidal Neovascularization / Q000473:pathology*; D004195:Disease Models, Animal; D007834:Lasers / Q000009:adverse effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009389:Neovascularization, Pathologic