Hepatitis C virus genotypes in Hungarian and Austrian patients with chronic hepatitis C.
Hepatitis C virus (HCV) genotypes are relevant to epidemiological questions, vaccine development, and clinical management of chronic HCV infection. The aim of this study was to determine HCV genotypes of South Hungarian and Southeast Austrian patients with chronic hepatitis C. Results were obtained by the largely automated TruGene HCV 5'NC Genotyping Kit (Visible Genetics, Toronto, Ontario) and by phylogenetic analysis. All of the 20 Hungarian patients and 15 out of 20 Austrian patients were infected with genotype 1. The remaining Austrian patients were infected with genotypes 3 or 2. With the commercially available assay, it was not possible to determine the HCV subtype in a total of three patients. The TruGene HCV 5'NC Genotyping assay for the determination of HCV genotypes proved to be useful for a high-throughput routine diagnostic laboratory.
Müller
Zsófia
Z
Department of Clinical Microbiology, University of Szeged, Somogyi B. tér 1, H-6725, Szeged, Hungary. zsomu@freemail.hu
Deák
Judith
J
Ross
R Stefan
RS
Nagy
Elisabeth
E
Kovács
László
L
Roggendorf
M
M
Kessler
Harald H
HH
D000410:Alanine Transaminase / Q000097:blood; D001317:Austria / Q000453:epidemiology; D015415:Biomarkers; D003955:Diagnostic Tests, Routine; D016679:Genome, Viral; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D019698:Hepatitis C, Chronic / Q000201:enzymology / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic
D015415:Biomarkers; D011933:Reagent Kits, Diagnostic; D000410:Alanine Transaminase