Sustainable enzymatic preparation of polyaspartate using a bacterial protease.
Diethyl l-aspartate was polymerized by a bacterial protease from Bacillus subtilis (BS) in organic solvent at a temperature between 30 and 50 degrees C to yield alpha-linked poly(ethyl l-aspartate) having an M(w) of up to 3700 and a maximum polymer yield of 85%. The best polymerization conditions were the 40 degrees C polymerization of diethyl l-aspartate using 30% protease BS containing 4.5 vol % water in acetonitrile for 2 days. Poly(ethyl l-aspartate) was readily depolymerized by the enzyme into the oligomeric and monomeric l-aspartate in aqueous acetonitrile. Poly(sodium aspartate) prepared by the saponification of poly(ethyl l-aspartate) was readily biodegradable by activated sludge obtained from the municipal sewage treatment plant. Also, poly(sodium aspartate) was depolymerized by the hydrolase enzyme into the monomeric aspartate. These results may indicate the sustainable chemical recycling and biorecycling of this polymer.
Soeda
Yasuyuki
Y
Faculty of Science and Technology, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
Toshima
Kazunobu
K
Matsumura
Shuichi
S
D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000201:enzymology*; D001673:Biodegradation, Environmental; D001704:Biopolymers / Q000096:biosynthesis* / Q000737:chemistry; D002384:Catalysis; D010450:Endopeptidases / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001704:Biopolymers; D001224:Aspartic Acid; D010450:Endopeptidases