Levels of malondialdehyde, glutathione and ascorbic acid in idiopathic thrombocytopaenic purpura.
BACKGROUND
Idiopathic thrombocytopaenic purpura (ITP) is an autoimmune disorder, which causes an acute or chronic thrombocytopenia, and may result in potentially life-threatening haemorrhage. Oxidative damage may be involved in the pathogenesis of autoimmune diseases. Antibodies to bind to membrane lipids and platelet destruction may play a role on lipid peroxidation in ITP.


OBJECTIVE
To investigate the posible role of lipid peroxidation and antioxidants in patients with ITP.


METHODS
The levels of plasma and erythrocyte malondialdehyde (MDA), erythrocyte glutathione and ascorbic acid were analysed in patients with ITP. METHODS. The MDA levels were performed according to the method of Bidlack WR. Plasma MDA, erythrocyte glutathione and ascorbic acid levels were carried out according to the methods of Ohkawa H, Beutler E and Bauer JD, respectively.


RESULTS
The erythrocyte and plasma MDA levels in patients with ITP were found to be 9.52+/-4.65, 3.03+/-1.44 (p<0.001) and in control group were found to be 2.49+/-0.57, 1.03+/-0.28 nmol/ml (p<0.001), respectively. Erythrocyte glutathione was found to be 3.71+/-0.82, 6.26+/-0.66 micromol/gr Hb (p<0.001). Ascorbic acid levels of these groups were 1.09+/-0.25, 1.70+/-0.33 mg/dl (p<0.001).


CONCLUSIONS
The oxidative damage is involved in the pathogenesis of ITP. In patients with ITP, the platelet destruction and bleeding may play significant role on elevation of lipid peroxidation and reduction of antioxidant capacity. Further studies on oxidant and antioxidant status of ITP are also needed to confirm these results.
Polat
G
G
Department of Biochemistry, Mersin University, Medical Faculty, Adana, Turkey.
Tamer
L
L
Tanriverdi
K
K
Gürkan
E
E
Baslamisli
F
F
Atik
U
U
D000328:Adult; D000975:Antioxidants; D001205:Ascorbic Acid / Q000032:analysis* / Q000097:blood*; D016022:Case-Control Studies; D004912:Erythrocytes / Q000737:chemistry*; D005260:Female; D005978:Glutathione / Q000032:analysis* / Q000097:blood*; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000032:analysis* / Q000097:blood*; D008875:Middle Aged; D018384:Oxidative Stress; D010976:Platelet Count; D011379:Prognosis; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000276:immunology / Q000378:metabolism*
D000975:Antioxidants; D008315:Malondialdehyde; D005978:Glutathione; D001205:Ascorbic Acid