Identification of novel SNPs in the interleukin 6 receptor gene (IL6R).
The human interleukin-6 receptor (IL6R) is responsible for signal transduction of IL6 that might have associations with immune diseases and various infectious diseases. We have sequenced all 10 exons including the putative splicing site to identify single nucleotide polymorphisms in IL6R. Seven novel single nucleotide polymorphisms (SNPs) were identified; one SNP in the promoter region (-183G>A), one synonymous SNP in exon 2 (24013G>A: Ala31Ala), one non-synonymous SNP in exon 9 (48892A>C: Asp358Ala) and four in introns (29753A>C, 42700T>C, 48869T>A and 59818C>T). The frequencies of each SNP in the Korean population (n=300) were 0.48 (-183 G>A), 0.13 (24013 G>A), 0.41 (29753A>C), 0.41 (42700T>C), 0.1 (48869T>A), 0.42 (48892A>C), and 0.07 (59818C>T), respectively. Haplotypes and their frequencies were estimated by the EM algorithm. Linkage disequilibrium coefficients (/D'/) between each SNP pair were also calculated. The information on SNPs and haplotypes in IL6R would be useful for genetic studies of this gene.
Kim
Lyoung Hyo
LH
Department of Genetic Epidemiology, SNP Genetics, Inc., 11th Floor, Maehun B/D, 13 Chongro 4 Ga, Chongro Gu, Seoul, 110-834, Korea.
Lee
Hyo-Suk
HS
Kim
Yoon Jun
YJ
Jung
Ji Hyun
JH
Kim
Jun Yeun
JY
Park
Byung Lae
BL
Shin
Hyoung Doo
HD
D002874:Chromosome Mapping / Q000379:methods; D005091:Exons / Q000235:genetics; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D022821:RNA Splice Sites / Q000235:genetics; D019947:Receptors, Interleukin-6 / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods
D022821:RNA Splice Sites; D019947:Receptors, Interleukin-6