Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis.
The solubilizing power of various nonionic and zwitterionic detergents as membrane protein solubilizers for two-dimensional electrophoresis was investigated. Human red blood cell ghosts and Arabidopsis thaliana leaf membrane proteins were used as model systems. Efficient detergents could be found in each class, i.e. with oligooxyethylene, sugar or sulfobetaine polar heads. Among the commercially available nonionic detergents, dodecyl maltoside and decaethylene glycol mono hexadecyl ether proved most efficient. They complement the more classical sulfobetaine detergents to widen the scope of useful detergents for the solubilization of membrane proteins in proteomics.
Luche
Sylvie
S
CEA - Laboratoire de Bioénergétique Cellulaire et Pathologique, EA 2943, DRDC/BECP, CEA - Grenoble, France.
Santoni
Véronique
V
Rabilloud
Thierry
T
D017360:Arabidopsis / Q000378:metabolism; D001622:Betaine / Q000737:chemistry; D002592:Cetomacrogol / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004910:Erythrocyte Membrane / Q000378:metabolism; D005030:Ethylenes / Q000737:chemistry; D005960:Glucosides / Q000494:pharmacology; D006801:Humans; D007477:Ions; D007525:Isoelectric Focusing; D008565:Membrane Proteins / Q000737:chemistry*; D017830:Octoxynol / Q000494:pharmacology; D018515:Plant Leaves / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*
D003902:Detergents; D005030:Ethylenes; D005960:Glucosides; D007477:Ions; D008565:Membrane Proteins; D011506:Proteins; D001622:Betaine; C040358:dodecyl maltoside; D017830:Octoxynol; D002592:Cetomacrogol