Inhibitory effect of aqueous extract from the gall of Rhus chinensis on alpha-glucosidase activity and postprandial blood glucose.
The present study examined the inhibitory effect of aqueous extract from the gall of Rhus chinensis (AEGRC) on alpha-glucosidase activity, an enzyme responsible for digestion of carbohydrate to monosaccharides in the process of intestinal absorption. AEGRC inhibited Bacillus alpha-glucosidase acitvity with an IC(50) of 0.9 micro g/ml. Its inhibition on alpha-glucosidase was determined to be noncompetitive and reversible when the enzyme-substrate mixture was simultaneously treated with AEGRC as an inhibitor. In addition, when it was orally administered to rats with sucrose (2g/kg), AEGRC (250-1000mg/kg) significantly suppressed the increase of blood glucose levels after sucrose loading in a dose dependent manner. These results suggest that AEGRC might exert anti-diabetic effect by suppressing carbohydrate absorption from intestine, and thereby reducing the postprandial increase of blood glucose.
Shim
Young-Jun
YJ
Department of Pharmacology & BK21 Program for Medical Sciences, College of Medicine, Korea University, 136-705, Seoul, South Korea.
Doo
Ho-Kyung
HK
Ahn
Se-Young
SY
Kim
Yong-Suk
YS
Seong
Je-Kyung
JK
Park
In-Sun
IS
Min
Bon-Hong
BH
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D003956:Dialysis; D004186:Disaccharidases / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D065089:Glycoside Hydrolase Inhibitors; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D007700:Kinetics; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology; D019518:Postprandial Period / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D029045:Rhus / Q000737:chemistry*; D013395:Sucrose / Q000494:pharmacology
D001786:Blood Glucose; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D013395:Sucrose; D004186:Disaccharidases