title: Detection of base sequence changes in the CEBPD gene in human breast cancer cell lines and primary breast cancer isolates.
abstract: Single strand conformation polymorphism (SSCP) analysis was performed on genomic DNA from 97 human breast cancer samples and 10 breast cell lines to screen for mutations in the single exon C/EBPD gene. Three C --> T transitions resulting in silent mutations were detected in three individual breast cancer samples. One breast cancer sample also contained a G --> T transversion (Q253H). The SUM-52PE cell line contained an A --> T transversion (AAG --> TAG) resulting in a nonsense mutation (K180Stop). All mutations identified in genomic DNA isolates were in highly conserved regions of the C/EBPD gene. This study indicates that mutational alterations in the coding region of the C/EBPD gene are relatively uncommon in human breast cancer.
authors.affiliation: Department of Veterinary Biosciences, Ohio State Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Dahai
authors.initials: D
authors.lastname: Tang
authors.forename: Jim
authors.initials: J
authors.lastname: DeWille
mesh_terms: D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D051743:CCAAT-Enhancer-Binding Protein-delta; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D018807:Polymorphism, Single-Stranded Conformational; D014157:Transcription Factors; D014407:Tumor Cells, Cultured
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; C497252:CEBPD protein, human; D014157:Transcription Factors; D051743:CCAAT-Enhancer-Binding Protein-delta