Two glycosylation alterations of mouse intestinal mucins due to infection caused by the parasite Nippostrongylus brasiliensis.
The glycosylation alterations of mouse small intestinal mucins during a 12-day infectious cycle caused by the parasite Nippostrongylus brasiliensis have been studied. The guanidinium chloride insoluble mucins were isolated at day 0 to 12 from the small intestine of infected and non-infected C57BL/6 mice. The O-linked oligosaccharides were released by reductive beta-elimination from the mucins and separated into neutral, sialylated and sulfated fractions. All fractions were analyzed by monosaccharide composition analysis and the neutral oligosaccharides were structurally characterized by gas chromatography/mass spectrometry. Two oligosaccharides containing blood group H-type epitopes (Fucalpha1-2Gal-) were transiently expressed with a maximum at day 6. Additional oligosaccharides with the common structure HexNAc-Gal-3GalNAcol were transiently induced with a maximum at day 10. Northern blot analysis on total RNA showed a transient expression at day 4-6 of the Fut2 gene encoding a Fucalpha1-2 fucosyltransferase, probably responsible for the detected blood group H-type epitopes. Comparisons with the corresponding infection in rat studied previously, revealed structurally different alterations, although occurring as transient events in both species. Both showed an induced blood group-type transferase halfway through the infection (a blood group A transferase in rat) and an induced transferase adding a terminal GalNAc (to a sialic acid- containing epitope in rat) towards the end of the infection. These differences between closely related species suggest rapid evolutionary alterations in glycosyltransferase expression.
Holmén
Jessica M
JM
Department of Medical Biochemistry, Göteborg University, P.O. Box 440, SE-405 30 Gothenburg, Sweden.
Olson
Fredrik J
FJ
Karlsson
Hasse
H
Hansson
Gunnar C
GC
D000818:Animals; D002240:Carbohydrate Sequence; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006031:Glycosylation; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000469:parasitology*; D051379:Mice; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009559:Nippostrongylus / Q000502:physiology*; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017206:Strongylida Infections / Q000378:metabolism* / Q000469:parasitology; D013431:Sulfates / Q000378:metabolism
D009077:Mucins; D009844:Oligosaccharides; D012333:RNA, Messenger; D013431:Sulfates; D005647:Fucosyltransferases; C031692:galactoside 3-fucosyltransferase; D019158:N-Acetylneuraminic Acid