title: Functional EF-Tu with large C-terminal extensions in an E coli strain with a precise deletion of both chromosomal tuf genes.
abstract: An Escherichia coli strain was constructed in which both chromosomal genes encoding elongation factor (EF)-Tu (tufA and tufB) have been inactivated with precise coding sequence replacements. A tufA gene in an expression vector is supplied as the sole EF-Tu source. By using plasmid replacement, based on plasmid incompatibility, mutant EF-Tu variants with a large C'-terminal extension up to 270 amino acids were studied and proved to be functional in a strain lacking the chromosomal tufA and tufB genes.
authors.affiliation: Department of Microbiology, Stockholm University, S-106 91 Stockholm, Sweden.
authors.forename: Robert
authors.initials: R
authors.lastname: Schnell
authors.forename: Farhad
authors.initials: F
authors.lastname: Abdulkarim
authors.forename: Miklós
authors.initials: M
authors.lastname: Kálmán
authors.forename: Leif A
authors.initials: LA
authors.lastname: Isaksson
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D017353:Gene Deletion; D010444:Peptide Elongation Factor Tu / Q000235:genetics*; D010957:Plasmids
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D010444:Peptide Elongation Factor Tu