title: Cytotoxic and antitumor activities of a polypore macrofungus, Phellinus rimosus (Berk) Pilat.
abstract: Cytotoxic and antitumor activities of ethyl acetate, methanol and aqueous extracts of a wood inhabiting polypore macrofungus, Phellinus rimosus (Berk) Pilat. were studied. Ethyl acetate and methanol extracts showed in vitro cytotoxic activity against Dalton's lymphoma ascites (DLA) and Ehrlich's ascites carcinoma (EAC) cell lines. The aqueous extract did not exhibit cytotoxicity against the tested cell lines. All the three extracts were highly effective in inhibiting growth of solid tumor induced by DLA cell line in mice. However, the antitumor activity of ethyl acetate extract was higher than that of methanol and aqueous extracts. The ethyl acetate extract was also effective in preventing the EAC induced ascites tumor development in mice. The antitumor activity of all the three extracts against solid tumor at a dose of 50 mg/kg (p.o.) was comparable to the clinically used standard reference drug, cisplatin (4 mg/kg, i.p.). Pre-treatment of the extracts was also effective in inhibiting the tumor growth induced by DLA cell lines. The experimental results revealed that ethyl acetate extract of P. rimosus possessed significant antitumor activity. The findings thus suggest the potential use of this mushroom as antitumor agent.
authors.affiliation: Amala Cancer Research Centre, Amala Nagar, Kerala 680 553, Thrissur, India.
authors.forename: T A
authors.initials: TA
authors.lastname: Ajith
authors.forename: K K
authors.initials: KK
authors.lastname: Janardhanan
mesh_terms: D000085:Acetates; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy / Q000473:pathology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D008297:Male; D000432:Methanol; D051379:Mice; D009368:Neoplasm Transplantation; D012997:Solvents; D014407:Tumor Cells, Cultured; D014867:Water
chemical_list: D000085:Acetates; D000903:Antibiotics, Antineoplastic; D012997:Solvents; D014867:Water; C007650:ethyl acetate; D000432:Methanol