title: An EREBP/AP2-type protein in Triticum aestivum was a DRE-binding transcription factor induced by cold, dehydration and ABA stress.
abstract: We characterize one transcription factor of DRE-binding proteins (TaDREB1) that was isolated from a drought-induced cDNA library of wheat (Triticum aestivum L.). TaDREB1 contains one conserved EREBP/AP2 domain, and shows similarity with Arabidopsis thaliana DREB family members in both overall amino-acid sequences and the secondary structure arrangement within the DNA-binding motifs. In yeast one-hybrid system, TaDREB1, can specially activate the genes fused with the promoter containing three tandemly repeated copies of the wild-type DRE sequence: TACCGACAT. In different wheat cultivars, the Ta DREB1 gene is induced by low temperature, salinity and drought; and the expression of Wcs120 that contains DRE motifs in its promoter is closely related to the expression of TaDREB1. These results suggest that TaDREB1 functions as a DRE-binding transcription factor in wheat. We also observed the dwarf phenotype in transgenic rice (T0) overexpressing TaDREB1.
authors.affiliation: Plant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Y-G
authors.initials: YG
authors.lastname: Shen
authors.forename: W-K
authors.initials: WK
authors.lastname: Zhang
authors.forename: S-J
authors.initials: SJ
authors.lastname: He
authors.forename: J-S
authors.initials: JS
authors.lastname: Zhang
authors.forename: Q
authors.initials: Q
authors.lastname: Liu
authors.forename: S-Y
authors.initials: SY
authors.lastname: Chen
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D001483:Base Sequence; D003080:Cold Temperature; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D003681:Dehydration / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014908:Triticum / Q000235:genetics / Q000378:metabolism*
chemical_list: C499534:APETALA2 protein, Arabidopsis; D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C114578:DREB1A protein, Arabidopsis; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D010940:Plant Proteins; D014157:Transcription Factors; C110867:Wcs120 protein, Triticum aestivum; C093680:ethylene-responsive element binding protein; D000040:Abscisic Acid