Analysis of the ferric citrate transport gene promoter of Escherichia coli.
FecI, an extracytoplasmic-function sigma factor, is required for initiation of transcription of the ferric citrate transport genes. A mutational analysis of the fecA promoter revealed that the nonconserved -10 region and a downstream regulatory element are important for fecA promoter activity. However, nucleotide substitutions in the well-conserved -35 region also have an effect on the fecA promoter activity. Titration of FecI suggests that the FecI-RNA polymerase holoenzyme does not bind strongly to the downstream regulatory element, which is therefore probably involved in a subsequent step of transcription initiation.
Enz
Sabine
S
Mikrobiologie/Membranphysiologie, Universität Tübingen, Germany.
Mahren
Susanne
S
Menzel
Claudia
C
Braun
Volkmar
V
D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D005290:Ferric Compounds / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D024363:Transcription Initiation Site
D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C028589:FecA protein, E coli; D005290:Ferric Compounds; D008164:Luminescent Proteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; C025314:ferric citrate