title: The kinase insert domain-containing receptor (KDR) is regulated by shear stress.
abstract: OBJECTIVE
  Intimal hyperplasia develops in areas with low shear stress. The aim of the present study was to investigate if the mRNA expression of vascular endothelial growth factor (VEGF), Fms-like tyrosine kinase-1 receptor (Flt-1) and kinase insert domain-containing receptor (KDR) is regulated by shear stress.
  
  
  METHODS
  Endothelial cells from human umbilical veins were in an in vitro system subjected to different levels of shear stress during 1 and 12 h. The mRNA expression of VEGF, Flt-1 and KDR was measured with RT-PCR. eNOS served as positive control and actin as housekeeping gene.
  
  
  RESULTS
  The KDR expression was isolated upregulated 3-4 times after 12 h exposure to high shear stress.
  
  
  CONCLUSIONS
  The genetic expression of KDR is upregulated by shear stress and this response is time dependent.
authors.affiliation: Department of Vascular Surgery, The Wallenberg Laboratory, Bruna Stråket 11B, Plan 1, Sahlgrenska University Hospital, SE-413 45 Gothenburg, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Staalesen
authors.forename: B
authors.initials: B
authors.lastname: Risberg
authors.forename: E
authors.initials: E
authors.lastname: Mattsson
mesh_terms: D002478:Cells, Cultured; D016228:Endothelial Growth Factors / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology; D018056:Hemorheology / Q000379:methods; D006801:Humans; D006965:Hyperplasia / Q000209:etiology; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis*; D008222:Lymphokines / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017539:Tunica Intima / Q000473:pathology; D014471:Umbilical Veins / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000096:biosynthesis; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000096:biosynthesis*; D042442:Vascular Endothelial Growth Factors
chemical_list: D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D040281:Vascular Endothelial Growth Factor Receptor-1; D040301:Vascular Endothelial Growth Factor Receptor-2