Performance characteristics of a rapid new immunochromatographic test for detection of antibodies to human immunodeficiency virus.
A new immunochromatographic rapid test (Rapid Check HIV 1 and 2; Núcleo de Doenças Infecciosas) for the detection of antibodies to human immunodeficiency virus type 1 and type 2 in human samples (whole blood, serum, and plasma) was evaluated and compared to the commercially available Determine (Abbott Laboratories). When whole-blood samples were evaluated, the specificity and sensitivity of both tests were 100%. However, when plasma samples were used, sensitivity for the Rapid Check HIV 1&2 and the Determine tests were 100 and 98.58%, respectively. The observed specificity for plasma samples was 98.94% for the Rapid Check HIV 1&2 and 96.97% for the Determine test. The results presented here are encouraging and support the adoption of both tests as an alternative to enzyme-lined immunosorbent assay and/or Western blots in regions where laboratorial infrastructure is not available or for use in the management of occupational accidents for healthcare workers.
Ribeiro-Rodrigues
Rodrigo
R
Cellular and Molecular Immunology Laboratory, Núcleo de Doenças Infecciosas, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil. rodrigrr@ndi.ufes.br
Ferreira da Silva Pinto Neto
Lauro
L
Cunha
Carla B
CB
Cabral
Valéria P
VP
Dietze
Reynaldo
R
D015658:HIV Infections / Q000175:diagnosis* / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology* / Q000302:isolation & purification; D015498:HIV-2 / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000592:standards; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic