Age-related base excision repair activity in mouse brain and liver nuclear extracts.
To assess DNA repair activity relative to age, in vitro base excision repair assays were performed using brain and liver nuclear extracts prepared from mice of various ages. An 85% decline in repair activity was observed in brain nuclear extracts and a 50% decrease in liver nuclear extracts prepared from old mice compared with 6-day-old mice. Brain nuclear extracts prepared from old mice showed a decreased abundance of DNA polymerase-beta, but the addition of purified protein did not restore base excision repair activity. Abundances of other tested base excision repair proteins did not change relative to age. The conclusion is that, during aging, a decline in DNA repair could contribute to increased levels of DNA damage and mutagenesis.
Intano
Gabriel W
GW
Department of Cellular & Structural Biology, The University of Texas Health Science Center at San Antonio, 78229, USA.
Cho
Eun Ju
EJ
McMahan
C Alex
CA
Walter
Christi A
CA
D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D045647:DNA Glycosylases; D011088:DNA Ligases / Q000494:pharmacology; D019951:DNA Polymerase beta / Q000378:metabolism; D004260:DNA Repair; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D051981:Uracil-DNA Glycosidase
D019951:DNA Polymerase beta; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase; D011088:DNA Ligases