Gene silencing by adenovirus-delivered siRNA.
RNA interference is the process that double-stranded RNA induces the homology-dependent degradation of cognate mRNA mediated by 21-23 nucleotide short interfering RNA (siRNA). Here, we describe a simple virus vector for efficient delivery of siRNA into mammalian cells utilizing the well-defined H1-RNA promoter and conventional adenovirus. In this pilot study, p53 was targeted by this vector. Our results demonstrate efficient and specific knock-down of p53 in breast cancer MCF-7 and lung carcinoma A549 cells and indicate a prospective application of this siRNA expressing recombinant adenovirus system in functional genomics, cancer gene therapy and virus inhibition.
Shen
Changxian
C
Department of Nuclear Medicine, University of Ulm, Robert-Koch-Strasse 8, D-89070 Ulm, Germany.
Buck
Andreas K
AK
Liu
Xiangwei
X
Winkler
Michael
M
Reske
Sven N
SN
D000256:Adenoviridae / Q000235:genetics; D016158:Genes, p53; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D014407:Tumor Cells, Cultured