title: Molecular markers in ductal carcinoma in situ of the breast.
abstract: Gene expression patterns in ductal carcinoma in situ (DCIS), and in invasive, and metastatic breast tumors were determined using serial analysis of gene expression (SAGE). We used mRNA in situ hybridization to examine gene expression at the cellular level and immunohistochemistry on tissue microarrays to determine association between gene expression patterns and histopathologic characteristics of the tumors. We found that that the most dramatic transcriptome change occurs at the normal to DCIS transition, while there is no clear universal "in situ" or "invasive" tumor molecular signature. From the 16,430 transcripts analyzed, we identified only 5 and 11 that were preferentially up-regulated in DCIS and invasive tumors, respectively. The majority of invasive cancer specific SAGE tags correspond to novel genes. The genes we identified may define biologically and clinically meaningful subgroups of DCIS with a high risk of progression to invasive disease.
authors.affiliation: Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
authors.forename: Dale
authors.initials: D
authors.lastname: Porter
authors.forename: Jaana
authors.initials: J
authors.lastname: Lahti-Domenici
authors.forename: Aparna
authors.initials: A
authors.lastname: Keshaviah
authors.forename: Young Kyung
authors.initials: YK
authors.lastname: Bae
authors.forename: Pedram
authors.initials: P
authors.lastname: Argani
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Marks
authors.forename: Andrea
authors.initials: A
authors.lastname: Richardson
authors.forename: Amiel
authors.initials: A
authors.lastname: Cooper
authors.forename: Robert
authors.initials: R
authors.lastname: Strausberg
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Riggins
authors.forename: Stuart
authors.initials: S
authors.lastname: Schnitt
authors.forename: Edward
authors.initials: E
authors.lastname: Gabrielson
authors.forename: Rebecca
authors.initials: R
authors.lastname: Gelman
authors.forename: Kornelia
authors.initials: K
authors.lastname: Polyak
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D005819:Genetic Markers; D006801:Humans; D007150:Immunohistochemistry; D020411:Oligonucleotide Array Sequence Analysis; D015854:Up-Regulation / Q000235:genetics
chemical_list: D005819:Genetic Markers