title: [An observation of the basement membrane remodeling after the combined grafting of xenogenic acellular dermal matrix with autoskin in rats].
abstract: OBJECTIVE
  To observe the dynamic process of basement membrane remodeling after the combined grafting of xenogenic acellular dermal matrix with autoskin.
  
  
  METHODS
  The rat skin wounds were covered with xenogenic porcine acellular dermal matrix overlaid with razor thin autoskin. The skin samples were collected at 1, 2, 3, 4, 8, 12 and 16 post-grafting weeks. The changes in laminin expression in the basement membrane and the ultrastructure of the basement membrane at 12 post-grafting weeks were observed by immunohistochemistry and transmission electron microscopy. The results were compared with those in simple thin autoskin grafting as the control.
  
  
  RESULTS
  The laminin expression in the combined grafting was higher than that in control. At 12 post-grafting weeks, the basement membrane in combined grafting rats was clear and continuous and the hemidesmosome was relatively more in amount and distributed evenly. While in the autoskin group, the lamina densa in the basement membrane was blurred and discontinuous with a decrease in and uneven distribution of hemidesmosome.
  
  
  CONCLUSIONS
  The increased expression of laminin in the basement membrane in the combined grafting rats might be beneficial to the remodeling of the basement membrane and to strengthening the connection of epithelium to the dermis, thus wound healing quality would be improved.
authors.affiliation: Burn Institute of Shanghai, Rui Jin Hospital, Shanghai Second Medical University, Shanghai 200025 P.R. China.
authors.forename: Qiuhe
authors.initials: Q
authors.lastname: Wu
authors.forename: Min
authors.initials: M
authors.lastname: Yao
authors.forename: Chun
authors.initials: C
authors.lastname: Qing
authors.forename: Qidong
authors.initials: Q
authors.lastname: Cao
authors.forename: Weishi
authors.initials: W
authors.lastname: Xu
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000378:metabolism / Q000648:ultrastructure; D002056:Burns / Q000601:surgery*; D020405:Dermis / Q000637:transplantation*; D007150:Immunohistochemistry; D007797:Laminin / Q000096:biosynthesis; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar; D016038:Skin Transplantation / Q000379:methods*; D013552:Swine; D013997:Time Factors; D014182:Transplantation, Autologous; D014183:Transplantation, Heterologous; D014945:Wound Healing
chemical_list: D007797:Laminin