title: [Constituent of natural food additive hokosshi extract and an analytical method for the additive in foods].
abstract: Hokosshi extract is obtained by ethanol extraction from the seeds of hokosshi (Psoralea corylifolia), which is used as a Chinese medicine. The constituents of hokosshi extract were analyzed. The main constituent was isolated using column chromatography, and identified as bakuchiol by TLC, LC/MS and NMR. Bavachinin A was also detected. In order to prepare a marker substance for hokosshi extract, bakuchiol was isolated from seeds of hokosshi using Sep-Pak cartridges. An analytical method for hokosshi extract in foods based on detection of bakuchiol was developed. Bakuchiol was extracted from food with 60 vol% ethanol. The extract was cleaned up using a Sep-Pak plus C18 cartridge, and bakuchiol was determined by HPLC. Seasoning and juice were spiked with hokosshi extract at 500 micrograms/g and analyzed by the proposed method. The recoveries of bakuchiol were 72-99%. The detection limit for the assay was 25 micrograms/g.
authors.affiliation: National Institute of Health Sciences: 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Chino
authors.forename: Kyoko
authors.initials: K
authors.lastname: Sato
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Tamio
authors.initials: T
authors.lastname: Maitani
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005503:Food Additives / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D010636:Phenols / Q000032:analysis*; D012639:Seeds / Q000737:chemistry
chemical_list: D005503:Food Additives; D010636:Phenols; C012765:bakuchiol