Oral immunization using tuber extracts from transgenic potato plants expressing rabbit hemorrhagic disease virus capsid protein.
Rabbit hemorrhagic disease, which is caused by a calicivirus, is a lethal infection of adult animals that is characterized by acute liver damage and disseminated intravascular coagulation. In this study, we report the production of the major structural protein VP60 of rabbit hemorrhagic disease virus in transgenic tubers of potato plants and its use as an oral immunogen in rabbits.
Martín-Alonso
José M
JM
Departamento de Biología de Organismos y Sistemas, Instituto Universitario de Biotecnología de Asturias (CNB-CSIC), Universidad de Oviedo, 33006 Oviedo, Spain.
Castañón
Sonia
S
Alonso
Pablo
P
Parra
Francisco
F
Ordás
Ricardo
R
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D017250:Caliciviridae Infections / Q000276:immunology / Q000517:prevention & control*; D002213:Capsid / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D017929:Hemorrhagic Disease Virus, Rabbit / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D030821:Plants, Genetically Modified; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D011198:Solanum tuberosum; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology*; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
D000956:Antigens, Viral; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D015678:Viral Structural Proteins; D014765:Viral Vaccines; C081149:viral protein 60, rabbit hemorrhagic disease virus