title: HLA-DRB1 allele polymorphisms in genetic susceptibility to esophageal carcinoma.
abstract: OBJECTIVE
  To probe into the genetic susceptibility of HLA-DRB1 alleles to esophageal carcinoma in Han Chinese in Hubei Province.
  
  
  METHODS
  HLA-DRB1 allele polymorphisms were typed by polymerase chain reaction with sequence-specific primers(PCR-SSP) in 42 unrelated patients with esophageal cancer and 136 unrelated normal control subjects and the associated HLA-DRB1 allele was measured by nucleotide sequence analysis with PCR.SAS software was used in statistics.
  
  
  RESULTS
  Allele frequency (AF) of HLA-DRB1*0901 was significantly higher in esophageal carcinoma patients than that in the normal controls (0.2500 vs 0.1397, P=0.028, the odds ratio 2.053, etiologic fraction 0.1282). After analyzed the allele nucleotide sequence of HLA-DRB1*0901 which approachs to the corresponded exon 2 sequence of the allele in genebank. There was no association between patients and controls in the rested HLA-DRB1 alleles.
  
  
  CONCLUSIONS
  HLA-DRB1*0901 allele is more common in the patients with esophageal carcinoma than in the healthy controls, which is positively associated with the patients of Hubei Han Chinese. Individuals carrying HLA-DRB1*0901 may be susceptible to esophageal carcinoma.
authors.affiliation: Department of Gastroenterology, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, China. linjun64@yahoo.com.cn
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.forename: Chang-Sheng
authors.initials: CS
authors.lastname: Deng
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.forename: Xian-Gong
authors.initials: XG
authors.lastname: Zheng
authors.forename: Xing
authors.initials: X
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Ping
authors.initials: P
authors.lastname: Xiong
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Wang
mesh_terms: D000328:Adult; D000483:Alleles; D002277:Carcinoma / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic
chemical_list: D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains