title: Structural analysis of the Lactobacillus rhamnosus strain KL37C exopolysaccharide.
abstract: The exopolysaccharide from the lactic acid bacterium Lactobacillus rhamnosus strain KL37C isolated from human intestinal flora was prepared by sonication of bacterial cell mass suspended in water followed by centrifugation and cold ethanol precipitation of the supernatant. The polysaccharide material was purified by gel permeation chromatography on an TSK HW-50 column and characterised using chemical and enzymatic methods. On the basis of sugar and methylation analysis and 1H, 13C, 1D and 2D NMR spectroscopy the exopolysaccharide was shown to be composed of the following pentasaccharide repeating unit:-->3)-alpha-D-Glcp-(1-->2)-beta-D-Galf-(1-->6)-alpha-D-Galp-(1-->6)-alpha-D-Glcp-(1-->3)-beta-D-Galf-(1-->
authors.affiliation: Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, PL-53 114, Wroclaw, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Lipiński
authors.forename: Christopher
authors.initials: C
authors.lastname: Jones
authors.forename: Xavier
authors.initials: X
authors.lastname: Lemercinier
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Korzeniowska-Kowal
authors.forename: Magdalena
authors.initials: M
authors.lastname: Strus
authors.forename: Jacek
authors.initials: J
authors.lastname: Rybka
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
authors.forename: Piotr B
authors.initials: PB
authors.lastname: Heczko
mesh_terms: D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000737:chemistry; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D007778:Lactobacillus / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D006023:Glycoproteins; D011135:Polysaccharides, Bacterial; D019344:Lactic Acid