title: Fluorescence imaging of lipid peroxidation in isolated rat lungs during nonhypoxic lung ischemia.
abstract: We have shown previously that ischemia results in reactive oxygen species production by lung endothelium that occurs within 3-5 s after flow cessation and is followed by lipid peroxidation at 15-30 min as determined by assay of thiobarbituric acid-reactive substances, conjugated dienes, and protein carbonyls in lung homogenate. The present study evaluated membrane lipid peroxidation in isolated, ventilated rat lungs using a fluorescence imaging method that permits continuous observation of pulmonary subpleural microvascular endothelial cells in situ. Diphenyl-1-pyrenylphosphine (DPPP), a fluorescent probe which localizes in the plasma membrane and shows increased fluorescence emission after its oxidation by lipid hydroperoxides, was used for detection of membrane lipid peroxidation. Compared to continuously perfused control lungs, endothelial cell DPPP fluorescence increased significantly within 1 min of ischemia (i.e., flow cessation); these changes were prevented by pretreatment with 0.5 mM alpha-tocopherol succinate (vitamin E) added to the perfusate. Increased DPPP fluorescence was confirmed by spectrofluorometry of lipid extracts of lung homogenates. These data indicate that DPPP can be used for the real-time detection of lipid peroxidation in an intact organ. Ischemia results in peroxidation of the pulmonary microvascular endothelial cell membrane and this insult can be detected as early as 1 min after the onset of ischemia compatible with a radical-mediated process.
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, Hadassah University Medical Center, Jerusalem, Israel.
authors.forename: Idit
authors.initials: I
authors.lastname: Matot
authors.forename: Yefim
authors.initials: Y
authors.lastname: Manevich
authors.forename: Abu Bakr
authors.initials: AB
authors.lastname: Al-Mehdi
authors.forename: Chun
authors.initials: C
authors.lastname: Song
authors.forename: Aron B
authors.initials: AB
authors.lastname: Fisher
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D004730:Endothelium, Vascular; D005453:Fluorescence; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D000860:Hypoxia / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007425:Intracellular Membranes / Q000737:chemistry* / Q000378:metabolism; D007511:Ischemia / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism; D008168:Lung / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009943:Organophosphorus Compounds; D016877:Oxidants / Q000494:pharmacology; D009195:Peroxidase / Q000378:metabolism; D011721:Pyrenes; D051381:Rats; D017207:Rats, Sprague-Dawley; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D005456:Fluorescent Dyes; D008054:Lipid Peroxides; D009943:Organophosphorus Compounds; D016877:Oxidants; D011721:Pyrenes; C066322:diphenyl-1-pyrenylphosphine; D014810:Vitamin E; D009195:Peroxidase