title: Visual detection of AMP and real-time monitoring of cyclic nucleotide phosphodiesterase (PDE) activity in neutral aqueous solution. Chemosensor-coupled assay of PDE and PDE inhibitors.
abstract: A phosphate chemosensor ensemble, [Zn(H-bpmp)(pyrocatechol violet)](+) is useful for a real-time assay of phosphodiesterases (PDEs) in a neutral aqueous solution. In addition, a simple and convenient screen and assay procedures for inhibitors of PDEs have been demonstrated using IBMX, a nonselective PDE inhibitor as an example.
authors.affiliation: Center for Integrated Molecular Systems and Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology, San 31 Hyojadong, Pohang 790-784, South Korea.
authors.forename: Min Su
authors.initials: MS
authors.lastname: Han
authors.forename: Dong H
authors.initials: DH
authors.lastname: Kim
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000378:metabolism*; D002151:Calorimetry; D007202:Indicators and Reagents; D007700:Kinetics; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
chemical_list: D007202:Indicators and Reagents; D010726:Phosphodiesterase Inhibitors; D012996:Solutions; D000249:Adenosine Monophosphate; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D015056:1-Methyl-3-isobutylxanthine