title: [Significance of expression of epidermal growth factor (EGF) and its receptor (EGFR) in chronic cholecystitis and gallbladder carcinoma].
abstract: OBJECTIVE
  Epidermal growth factor(EGF) and its receptor(EGFR) play an important role in tumorigenesis. Cholelithiasis and cholecystitis were closely related to gallbladder carcinoma. This study was designed to investigate the relationship between the expression of EGF and EGFR in chronic cholecystitis and gallbladder carcinoma.
  
  
  METHODS
  SABC immunohistochemistry was used to determine the expression of EGF, EGFR, and proliferating cell nuclear antigen (PCNA) in surgically resected specimens of 41 gallbladder carcinomas, 26 simple hyperplasia tissues, 14 dysplasia tissues,and 10 normal gallbladder tissues.
  
  
  RESULTS
  The positive expression rates of EGF and EGFR were higher in gallbladder carcinomas(63.4%,70.7%) and dysplasia tissues (71.4%,85.7%) than in simple hyperplasia tissues (15.4%,27%) and normal gallbladder tissues (0%,0%)(P< 0.01); PCNA counting score tended to increase with the severity of mucosa change degree(P< 0.01), which were 1.0 in normal tissues, 1.0 in simple hyperplasia tissues, 1.9288+/-0.9972 in dysplasia tissues,and 3.0488+/-0.669 in gallbladder carcinomas. Significantly positive correlation were shown between EGF,EGFR, and PCNA(P< 0.05). There was no correlation between EGF, EFGR, and PCNA expression and tumor TNM stage(P>0.05).
  
  
  CONCLUSIONS
  Expression of EGF and EGFR is involved in the gallbladder carcinogenesis, and is related to high activity of cell proliferation.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Lanzhou Medical College, Lanzhou, Gansu, 730000, P.R. China. zym766@hotmail.com
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Zhou
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Li
authors.forename: Nong
authors.initials: N
authors.lastname: Cao
authors.forename: Ying
authors.initials: Y
authors.lastname: Feng
authors.forename: Feng
authors.initials: F
authors.lastname: Zeng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002764:Cholecystitis / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D066246:ErbB Receptors / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005706:Gallbladder Neoplasms / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D004815:Epidermal Growth Factor; D066246:ErbB Receptors