Effect of bradykinin and PMA on the synthesis of proteoglycans during the cell cycle of endothelial cells in culture.
Bradykinin (BK) and phorbol 12-myristate-13-acetate (PMA) were used in the present work to study the biosynthesis of proteoglycans (PG) during the cell cycle of endothelial cells. PMA, an activator of PKC, stimulated the synthesis of heparan sulfate proteoglycan (HSPG) secreted to the medium of endothelial cells mainly during the G(1) phase of the cell cycle [J. Cell. Biochem. 70 (1998) 563]. BK is a vasoactive peptide that increases calcium levels inside the cells indirectly stimulating PKC. Treatment of the endothelial cells with BK, as well as PMA, stimulated the synthesis of HSPG secreted to the medium and produced an antimitogenic effect on the cell cycle. These results led to the conclusion that PKC is directly involved in the synthesis of HSPG secreted to the medium. Also, comparing the effect showed by BK with PMA, one may suggest that different PKC isoforms are involved in these two processes and that their isoforms are mainly Ca(2+) dependent.
Moreira
Claudia R
CR
Departamento de Bioquímica/Biologia Molecular, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua 3 de Maio 100, Quarto andar, CEP 04044-020 São Paulo, São Paulo, Brazil. crmoreira.bioq@epm.br
Porcionatto
Marimelia A
MA
Dietrich
Carl P
CP
Nader
Helena B
HB
D020001:1-Butanol / Q000494:pharmacology; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000096:biosynthesis / Q000378:metabolism; D006497:Heparan Sulfate / Q000096:biosynthesis; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011509:Proteoglycans / Q000096:biosynthesis*; D011817:Rabbits; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D020001:1-Butanol; D001920:Bradykinin; D004791:Enzyme Inhibitors; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D011493:Protein Kinase C; D011509:Proteoglycans; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine