Id-1 is not expressed in the luminal epithelial cells of mammary glands.
BACKGROUND
The family of inhibitor of differentiation/DNA binding (Id) proteins is known to regulate development in several tissues. One member of this gene family, Id-1, has been implicated in mammary development and carcinogenesis. Mammary glands contain various cell types, among which the luminal epithelial cells are primarily targeted for proliferation, differentiation and carcinogenesis. Therefore, to assess the precise significance of Id-1 in mammary biology and carcinogenesis, we examined its cellular localization in vivo using immunohistochemistry.


METHODS
Extracts of whole mammary glands from wild type and Id-1 null mutant mice, and tissue sections from paraffin-embedded mouse mammary glands from various developmental stages and normal human breast were subjected to immunoblot and immunohistochemical analyses, respectively. In both these procedures, an anti-Id-1 rabbit polyclonal antibody was used for detection of Id-1.


RESULTS
In immunoblot analyses, using whole mammary gland extracts, Id-1 was detected. In immunohistochemical analyses, however, Id-1 was not detected in the luminal epithelial cells of mammary glands during any stage of development, but it was detected in vascular endothelial cells.


CONCLUSIONS
Id-1 is not expressed in the luminal epithelial cells of mammary glands.
Uehara
Norihisa
N
Division of Life Sciences, Lawrence Berkeley National Laboratory, University of California Berkeley, USA.
Chou
Yu-Chien
YC
Galvez
Jose J
JJ
de-Candia
Paola
P
Cardiff
Robert D
RD
Benezra
Robert
R
Shyamala
Gopalan
G
D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D004847:Epithelial Cells / Q000737:chemistry* / Q000166:cytology; D007150:Immunohistochemistry; D051798:Inhibitor of Differentiation Protein 1; D008321:Mammary Glands, Animal / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D012097:Repressor Proteins; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
C497455:Idb1 protein, mouse; D051798:Inhibitor of Differentiation Protein 1; D012097:Repressor Proteins; D014157:Transcription Factors