Isolation and characterisation of a novel bacteriocin produced by Bacillus thuringiensis strain B439.
Bacillus thuringiensis strain B439 produces a bacteriocin-like inhibitory substance in its growth medium. This antimicrobial peptide, referred to as thuricin 439, acts as a bacteriocidal peptide and exhibits an apparent narrow range of inhibitory activity, essentially only affecting growth of Bacillus cereus and B. thuringiensis strains. It remains active over a relatively wide pH and temperature range, showing no loss of activity following heat treatments up to 80 degrees C. Purification of thuricin 439 was achieved using several chromatographic steps, which resulted in the identification of two peptides with inhibitory activity. These two peptides were shown to possess identical N-terminal sequences, but different molecular masses.
Ahern
Melissa
M
Department of Microbiology, National University of Ireland, Western Road, Cork, Ireland.
Verschueren
Saskia
S
van Sinderen
Douwe
D
D000595:Amino Acid Sequence; D001409:Bacillus cereus / Q000187:drug effects; D001413:Bacillus thuringiensis / Q000187:drug effects / Q000378:metabolism*; D001430:Bacteriocins / Q000302:isolation & purification* / Q000494:pharmacology; D002847:Chromatography, Agarose; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012685:Sepharose / Q000031:analogs & derivatives*; D013696:Temperature
D001430:Bacteriocins; C027176:octyl-sepharose CL-4B; D012685:Sepharose