title: Using second-order calibration to identify and quantify aromatic sulfonates in water by high-performance liquid chromatography in the presence of coeluting interferences.
abstract: We used the Generalized Rank Annihilation Method (GRAM), a second-order calibration method, to quantify aromatic sulfonates in water with high-performance liquid chromatography (HPLC) when interferences coeluted with the analytes of interest. With GRAM, we can quantify in only two chromatographic analyses, one for a calibration sample and one for the unknown sample. The calculated concentrations were not statistically different to those obtained when the chromatographic separation of the unknown sample was modified in order to completely separate the analyte from the interferences before univariate calibration. With GRAM, the concentrations are determined much more quickly because a complete resolution is not required.
authors.affiliation: Department of Analytical Chemistry and Organic Chemistry, Rovira i Virgili University, Imperial Tàarraco, 1, 43005 Tarragona, Spain.
authors.forename: Enric
authors.initials: E
authors.lastname: Comas
authors.forename: R Ana
authors.initials: RA
authors.lastname: Gimeno
authors.forename: Joan
authors.initials: J
authors.lastname: Ferré
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Marcé
authors.forename: Francesc
authors.initials: F
authors.lastname: Borrull
authors.forename: F Xavier
authors.initials: FX
authors.lastname: Rius
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results; D013451:Sulfonic Acids / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D013451:Sulfonic Acids; D014874:Water Pollutants, Chemical