Application of Agilent 2100 Bioanalyzer in detection of human papilloma virus.
OBJECTIVE
To explore the feasibility of using Agilent 2100 bioanalyzer in the detection and genotyping of human papilloma virus (HPV).


METHODS
The consensus sequence of highly conserved region (L1) and genotype-specific gene fragments of all HPV genotypes (including HPV6, 11, 16 and 18) were amplified by PCR technique using general and type-specific primers respectively. Agilent 2100 Bioanalyzer and routine agarose gel electrophoresis were then employed respectively to examine the PCR products, and the accuracy, reproducibility and sensitivity of these 2 detection techniques were compared.


RESULTS
Agilent 2100 Bioanalyzer showed better accuracy in determining the length of the gene fragments than agarose gel electrophoresis, the accuracy of the former reaching above 95% while the latter was only 85%. Bioanalyzer was also 100 times more sensitive than agarose electrophoresis.


CONCLUSIONS
Agilent 2100 Bioanalyzer combined with PCR is specific, accurate and sensitive for HPV detection and genotyping.
Liu
Cui-hua
CH
Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.
Ma
Wen-li
WL
Shi
Rong
R
Peng
Yi-fei
YF
Ouyang
Qian
Q
Zheng
Wen-ling
WL
D004587:Electrophoresis, Agar Gel / Q000379:methods; D006801:Humans; D027383:Papillomaviridae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic