Effects of ochratoxin A on micronucleus frequency in human lymphocytes.
Ochratoxin A (OTA), a nephrotoxic and carcinogenic mycotoxin, was investigated to examine its potency to induce micronuclei (MN) in cultured human lymphocytes. Lymphocyte cultures were treated for the last 48 h with OTA at concentrations of 25 microM, 10 microM, 1 microM, 100 nM, 10 nM, 1 nM, and 100 microM and absolute ethanol. At the highest concentration, OTA was found to induce MN in cytokinesis-blocked lymphocytes (p < 0.05). The 25 microM OTA concentration also led to a clear decrease in the percentage of binucleated cells, probably due to cytotoxicity. OTA at the other concentrations tested did not induce MN frequency. These results indicate that a high concentration of OTA is genotoxic in cultured human lymphocytes.
Dönmez-Altuntaş
Hamiyet
H
Erciyes University, Medical Faculty, Department of Medical Biology, Kayseri, Turkey med-biol@erciyes.edu.tr
Hamurcu
Zuhal
Z
Imamoglu
Nalan
N
Liman
Bilal Cem
BC
D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests; D009793:Ochratoxins / Q000494:pharmacology* / Q000633:toxicity
D009793:Ochratoxins; C025589:ochratoxin A