title: Comparison of the susceptibility testing of clinical isolates of Mycobacterium tuberculosis by the XTT colorimetric method and the NCCLS standards method.
abstract: The susceptibility or resistance of clinical isolates of Mycobacterium tuberculosis were determined by a method incorporating the 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and compared with results obtained by the National Committee for Clinical Laboratory Standards approved standard method (M24-T2). One hundred percent of all isolates demonstrated agreement between the susceptibility and resistance to isoniazid, rifampicin, and ethambutol obtained by the two methods, suggesting that the XTT-based method could provide a useful means for the rapid determination of antimycobacterial susceptibility of clinical isolates of M. tuberculosis.
authors.affiliation: Dipartimento di Scienze Chirurgiche e Trapianti d'Organo, Sezione di Microbiologia e Virologia, Università di Cagliari, Viale Frà Ignazio 38, 09123 Cagliari, Italy. adelogu@unica.it
authors.forename: Alessandro
authors.initials: A
authors.lastname: De Logu
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Pellerano
authors.forename: Adriana
authors.initials: A
authors.lastname: Sanna
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Pusceddu
authors.forename: Patrizia
authors.initials: P
authors.lastname: Uda
authors.forename: Barbara
authors.initials: B
authors.lastname: Saddi
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology; D015169:Colony Count, Microbial; D003124:Colorimetry / Q000379:methods; D024881:Drug Resistance, Bacterial; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D013778:Tetrazolium Salts; D018088:Tuberculosis, Multidrug-Resistant / Q000188:drug therapy / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide