[Preparation of monoclonal antibodies against oh(8)dG and their biological characteristics].
To prepare monoclonal antibodies against oh(8)dG and to evaluate the relationship between Hp infection and oxidative DNA damage by detecting oh8dG in gastric mucosa.



BALB/C mice were immunized with BSA-oh(8)dG conjugate, monoclonal antibodies were prepared by hybridoma technique, the biological characteristics of antibodies were analysed by competitive ELISA, Western blot and immunohistochemistry.



Two strains of hybridoma cell were obtained. ELISA and Western blot indicated that the antibodies were fairly specific for oh(8)dG. In immunohistochemistry,the positive rate of oh(8)dG expression in Hp positive tissues and Hp negative tissues was 55% and 5%, respectively(P<0.01).



The prepared antibodies can specially recognize oh(8)dG and immunohistochemistry with the monoclonal antibodies showed Hp infection can increase oh(8)dG level in gastric mucosa.
Wang
Jian
J
Department of Immunology, College of Medical Sciences, Zhejiang University   Hangzhou 310031, China.
Shen
Jian-Gen
JG
Zhu
Yong-Liang
YL
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005753:Gastric Mucosa / Q000737:chemistry; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine