[The role of C5a in adhesion properties of polymorphonuclear leukocyte to pulmonary vascular endothelial cells in burn patients with acute lung injury].
OBJECTIVE
To explore the postburn adhesion properties of polymorphonuclear leukocyte (PMN) onto pulmonary vascular endothelial cells (PVEC) in burn patients with acute lung injury (ALI), so as to determine the role of C5a on PVEC-PMN adhesion.


METHODS
Microtubule sucking technique was employed to determine the PVEC-PMN adhesion. The myeloperoxidase (MPO) was also assayed to reflect the magnitude of PVEC-PMN adhesion.


RESULTS
The magnitude of PVEC-PMN adhesion increased and the adhesion force increased along with an increase in rh-C5a concentration. Simultaneously, the MPO activity was increased, which could be inhibited by anti-C5aR McAb in a concentration 1:104.


CONCLUSIONS
Both C5a and C5aR participated in PVEC-PMN adhesion, which might be important in the pathogenesis of ALI.
Lu
Fenglin
F
Research Institute of Field Surgery, Da Ping Hospital, The Third Military Medical University, Chongqing, 400042 P.R. China.
Zhu
Xihua
X
Hu
Chengxiang
C
Huang
Yunhui
Y
D000208:Acute Disease; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015703:Antigens, CD / Q000276:immunology; D002056:Burns / Q000097:blood* / Q000150:complications; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D015936:Complement C5a / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D005333:Fetus; D006801:Humans; D008168:Lung; D008171:Lung Diseases / Q000150:complications*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D009195:Peroxidase / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000378:metabolism; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement; D015936:Complement C5a; D009195:Peroxidase