title: Expression of cyclooxygenase-2 in swine naturally infected with Actinobacillus pleuropneumoniae.
abstract: Cyclooxygenase-2 (COX-2) was detected and localized in 15 pigs with naturally occurring pleuropneumonia using a 437-base pair digoxigenin-labeled cDNA probe in an in situ hybridization protocol. Histopathologic changes in the acute stage were characterized by coagulative necrosis of lung parenchyma, hemorrhage, vascular thrombosis, edema, fibrin deposition, and infiltration of lung parenchyma by neutrophils and alveolar macrophages in nine pigs. In chronic lesions, a thick layer of granulation tissue surrounded foci of pulmonary necrosis in six pigs. All 15 pigs infected with Actinobacillus pleuropneumoniae, confirmed by bacterial isolation, had distinct positive hybridization signals for COX-2 in bronchial, bronchiolar epithelial cells, alveolar macrophages, neutrophils, and type I pneumocytes. COX-2 expression was detected primarily in neutrophils from pigs with acute lesions and primarily in alveolar macrophages from pigs with chronic lesions. The results suggest that a prostanoid product of COX-2 is an important component of the inflammatory response to acute and chronic A. pleuropneumoniae infection.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon, Republic of Korea.
authors.forename: W S
authors.initials: WS
authors.lastname: Cho
authors.forename: C
authors.initials: C
authors.lastname: Chae
mesh_terms: D000189:Actinobacillus Infections / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000254:growth & development*; D000208:Acute Disease; D000818:Animals; D051546:Cyclooxygenase 2; D015971:Gene Expression Regulation, Enzymologic; D017403:In Situ Hybridization / Q000662:veterinary; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008168:Lung / Q000473:pathology / Q000821:virology; D016676:Macrophages, Alveolar / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology; D011002:Pleuropneumonia, Contagious / Q000201:enzymology / Q000382:microbiology / Q000473:pathology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000201:enzymology* / Q000382:microbiology* / Q000473:pathology
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases