Effects of rosiglitazone and oleic acid on UCP-3 expression in L6 myotubes.
OBJECTIVE
The regulation of uncoupling protein-3 (UCP-3) expression in muscle remains unclear, specifically in relation to dietary and drug treatments. The present study evaluated the effects of oleic acid and rosiglitazone on UCP-3 mRNA expression in differentiated L6 myotubes.


METHODS
L6 myocytes were cultured and differentiated prior to exposure to rosiglitazone 10 micro mol/l, oleic acid 100 micro mol/l, or the combination, for 24 h, prior to semiquantitative evaluation of UCP-3 mRNA relative to GAPDH mRNA by RT-PCR.


RESULTS
Exposure to oleic acid produced a significant increase in UCP-3 mRNA (0.012 +/- 0.007 vs. 0.0011 +/- 0.0006 for untreated cells, relative to GAPDH mRNA, p < 0.001). Rosiglitazone alone had no effect on UCP-3 expression and nor did the glitazone affect oleic-acid-induced upregulation of UCP-3.


CONCLUSIONS
In L6 myotubes, 24-h exposure to oleic acid produced a 10-fold increase in UCP-3 mRNA expression, but rosiglitazone had no effect. Oleic-acid-induced upregulation of UCP-3 was not affected (positively or negatively) by glitazone exposure.
Costello
A
A
School of Medical and Surgical Sciences, University of Nottingham.
Gray
S
S
Donnelly
R
R
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D004338:Drug Combinations; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007473:Ion Channels; D024101:Mitochondrial Proteins; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D019301:Oleic Acid / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077154:Rosiglitazone; D013844:Thiazoles / Q000494:pharmacology*; D045162:Thiazolidinediones; D000071247:Uncoupling Protein 3
D002352:Carrier Proteins; D004338:Drug Combinations; D007004:Hypoglycemic Agents; D007473:Ion Channels; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D013844:Thiazoles; D045162:Thiazolidinediones; C000604394:Ucp3 protein, rat; D000071247:Uncoupling Protein 3; D000077154:Rosiglitazone; D019301:Oleic Acid