An efficient oxidizing reagent for the synthesis of mixed backbone oligonucleotides via the H-phosphonate approach.
The mixture of carbon tetrachloride, N-methyl morpholine (NMM), pyridine and water in acetonitrile has been exploited for the oxidation of dinucleoside H-phosphonate diesters to the corresponding phosphates. The system is found to be inert to the phosphoramidate (P-N) and the phosphorothioate (P-S) linkages and has successfully been applied to the solid phase synthesis of mixed-backbone oligonucleotides (MBOs).
Mohe
Nikhil U
NU
Department of Chemistry, The Institute of Science, 15-Madam Cama Road, 032, Mumbai-400, India
Padiya
Kamlesh J
KJ
Salunkhe
Manikrao M
MM
D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000138:chemical synthesis*; D063065:Organophosphonates / Q000737:chemistry*; D016877:Oxidants / Q000737:chemistry*; D010084:Oxidation-Reduction; D021241:Spectrometry, Mass, Electrospray Ionization
D007202:Indicators and Reagents; D009841:Oligonucleotides; D063065:Organophosphonates; D016877:Oxidants