Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis.
To increase the number of proteins detectable by two-dimensional electrophoresis (2-DE) in plants, we present a new procedure for extracting total proteins from plant tissue. This method avoids any loss of proteins in the course of sample preparation and results in two different fractions, one comprising mainly the cytoplasmatic proteins, the other one containing predominantly structure bond proteins. 2-DE patterns obtained from these two fractions show that the total number of different protein spots detected exceeds the degree of resolution commonly reported for plant proteins threefold.
Giavalisco
Patrick
P
Fachbereich Biologie, Freie Universität Berlin and Max Planck Institut für Molekulare Genetik, Germany. giavalis@molgen.mpg.de
Nordhoff
Eckhard
E
Lehrach
Hans
H
Gobom
Johan
J
Klose
Joachim
J
D029681:Arabidopsis Proteins / Q000302:isolation & purification*; D005591:Chemical Fractionation; D011232:Chemical Precipitation; D002843:Chromatin / Q000737:chemistry; D003902:Detergents; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D007525:Isoelectric Focusing; D012834:Silver; D013194:Staining and Labeling
D029681:Arabidopsis Proteins; D002843:Chromatin; D003902:Detergents; D012834:Silver