title: Fluorescence in situ hybridization (FISH) on human chromosomes using photoprobe biotin-labeled probes.
abstract: Fluorescence in situ hybridization (FISH) on human chromosomes in meta- and interphase is a well-established technique in clinical and tumor cytogenetics and for studies of evolution and interphase architecture. Many different protocols for labeling the DNA probes used for FISH have been published. Here we describe for the first time the successful use of Photoprobe biotin-labeled DNA probes in FISH experiments. Yeast artificial chromosome (YAC) and whole chromosome painting (wcp) probes were tested.
authors.affiliation: Institute of Human Genetics, Jena, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Weise
authors.forename: Peter
authors.initials: P
authors.lastname: Harbarth
authors.forename: Uwe
authors.initials: U
authors.lastname: Claussen
authors.forename: Thomas
authors.initials: T
authors.lastname: Liehr
mesh_terms: D001710:Biotin / Q000737:chemistry; D020223:Chromosome Painting / Q000379:methods; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D015342:DNA Probes / Q000737:chemistry; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*
chemical_list: D015342:DNA Probes; D001710:Biotin