Flow cytometric assessment of ethylene glycol monoethyl ether on spermatogenesis in rats.
The effects of ethylene glycol monoethyl ether (EGEE) on testicular cell populations in rats were investigated by a flow cytometric method. Rats were administered by gavage with EGEE at the various doses of 0 (saline alone), 100, 200, 400, and 800 mg/kg body weight/day for 4 weeks. The treatment of EGEE caused decreases in the weight of testis and epididymis and in the number of testicular cells. Histopathologically, exfoliation of germ cells into the tubular lumen was observed at the doses of above 200 mg/kg. The treatment of EGEE at the dose of 400 mg/kg caused moderate testicular degeneration. A significant depletion of haploid cells and a disproportionate ratio of diploid and tetraploid cells were observed as determined by flow cytometric analysis. These results indicate that the toxic effect of EGEE on the male reproductive system may be strongly associated with the disproportion of testicular germ cells.
Yoon
Chang Yong
CY
Department of Pathology, National Institute of Toxicological Research, Korea Food & Drug Administration, Seoul, Japan.
Hong
Choong Man
CM
Cho
Yong-Yeon
YY
Chung
Yong Hyun
YH
Min
Hong Ki
HK
Yun
Young Won
YW
Lee
Beom Jun
BJ
Yang
Ki Hwa
KH
Lee
Yong Soon
YS
Kim
Cheol Kyu
CK
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004247:DNA / Q000032:analysis; D005026:Ethylene Glycols / Q000633:toxicity*; D005434:Flow Cytometry; D008297:Male; D011003:Ploidies; D051381:Rats; D017207:Rats, Sprague-Dawley; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*; D013737:Testis / Q000166:cytology / Q000473:pathology
D005026:Ethylene Glycols; D004247:DNA; C021296:2-ethoxyethanol