title: FcgammaRIIIA-158V and rheumatoid arthritis: a confirmation study.
abstract: OBJECTIVE
  To develop a robust assay for genotyping the FcgammaRIIIA-158V/F polymorphism and to confirm the putative association between the FcgammaRIIIA-158V allele and rheumatoid arthritis (RA).
  
  
  METHODS
  This allelic association study examined the FcgammaRIIIA-158V/F polymorphism for association with RA. A novel single-stranded conformational polymorphism assay was used to genotype 828 RA patients and 581 controls from the UK.
  
  
  RESULTS
  The FcgammaRIIIA-158V allele was associated with both RA (P=0.02) and nodules (P=0.04). Individuals homozygous for this higher affinity allele had a significantly increased risk of RA (OR 1.53, 95% CI 1.08-2.18) and the development of nodules (OR 2.20, 95% CI 1.20-4.01). There was no evidence of an interaction with the shared epitope.
  
  
  CONCLUSIONS
  We have developed a novel assay to genotype the FcgammaRIIIA-158F/V polymorphism and confirmed that homozygosity for the FcgammaRIIIA-158V allele is associated with UK Caucasian RA, particularly in those individuals with nodules, suggesting FcgammaRIIIA may play a role in determining disease severity or in the development of nodules per se.
authors.affiliation: Rheumatology and Rehabilitation Research Unit, University of Leeds, UK. a.w.morgan@leeds.ac.uk
authors.forename: A W
authors.initials: AW
authors.lastname: Morgan
authors.forename: V H
authors.initials: VH
authors.lastname: Keyte
authors.forename: S J
authors.initials: SJ
authors.lastname: Babbage
authors.forename: J I
authors.initials: JI
authors.lastname: Robinson
authors.forename: F
authors.initials: F
authors.lastname: Ponchel
authors.forename: J H
authors.initials: JH
authors.lastname: Barrett
authors.forename: B B
authors.initials: BB
authors.lastname: Bhakta
authors.forename: S J
authors.initials: SJ
authors.lastname: Bingham
authors.forename: M H
authors.initials: MH
authors.lastname: Buch
authors.forename: P G
authors.initials: PG
authors.lastname: Conaghan
authors.forename: A
authors.initials: A
authors.lastname: Gough
authors.forename: M
authors.initials: M
authors.lastname: Green
authors.forename: C A
authors.initials: CA
authors.lastname: Lawson
authors.forename: C T
authors.initials: CT
authors.lastname: Pease
authors.forename: A F
authors.initials: AF
authors.lastname: Markham
authors.forename: W E R
authors.initials: WE
authors.lastname: Ollier
authors.forename: P
authors.initials: P
authors.lastname: Emery
authors.forename: J
authors.initials: J
authors.lastname: Worthington
authors.forename: J D
authors.initials: JD
authors.lastname: Isaacs
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000235:genetics*; D015331:Cohort Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006684:HLA-DR Antigens / Q000032:analysis; D059811:HLA-DRB1 Chains; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D017452:Receptors, IgG / Q000235:genetics*
chemical_list: D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D017452:Receptors, IgG