Detection of Cryptosporidium parvum and Giardia lamblia carried by synanthropic flies by combined fluorescent in situ hybridization and a monoclonal antibody.
Wild-caught synanthropic flies were tested for the presence of Cryptosporidium parvum and Giardia lamblia on their exoskeletons and in their digestive tracks by fluorescent in situ hybridization and fluorescein isothiocyanate (FITC)-conjugated monoclonal antibody (MAb) against Cryptosporidium and Giardia cell wall epitopes. The levels of C. parvum were positively correlated with the levels of G. lamblia, indicating a common source of contamination. The majority of oocysts and cysts were potentially viable (C. parvum = 80% and G. lamblia = 69%). More G. lamblia cysts occurred on the exoskeleton of the flies than within the digestive tracts; the opposite relationship was observed for C. parvum. No genotype other than C. parvum G2 was found to be associated with flies. Because filth flies carry viable C. parvum oocysts and G. lamblia cysts acquired naturally from unhygienic sources, they can be involved in the epidemiology of cryptosporidiosis and giardiasis. Fluorescent oligonucleotide probes used together with FITC-conjugated MAb represent a convenient and cost-effective technique for rapid and specific identification of human-infectious species of Cryptosporidium and Giardia mechanically transported by flies, and for the assessment of the viability of these pathogens.
Graczyk
Thaddeus K
TK
The W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA. tgraczyk@jhsph.edu
Grimes
Barbara H
BH
Knight
Ronald
R
Da Silva
Alexandre J
AJ
Pieniazek
Norman J
NJ
Veal
Duncan A
DA
D000818:Animals; D002417:Cattle; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000517:prevention & control* / Q000635:transmission; D016785:Cryptosporidium parvum / Q000235:genetics* / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000235:genetics; D003612:Dairying; D004175:Diptera / Q000469:parasitology*; D016829:Giardia lamblia / Q000235:genetics* / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology / Q000517:prevention & control* / Q000635:transmission; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007303:Insect Vectors / Q000469:parasitology*; D009657:North Carolina / Q000453:epidemiology; D018505:Waste Management
D016054:DNA, Protozoan