New ELISA system for myeloid-related protein complex (MRP8/14) and its clinical significance as a sensitive marker for inflammatory responses associated with transplant rejection.
BACKGROUND
C-reactive protein (CRP), a useful marker for inflammatory diseases, is not always sensitive to inflammatory reaction in the liver or other tissues. The aim of this study was to develop a sensitive and specific method for detecting inflammatory responses associated with transplant rejection.


METHODS
We developed a new, highly sensitive ELISA system for the measurement of serum human myeloid-related protein complex (MRP8/14), using monoclonal antibodies against MRP8/14, and applied it to specimens obtained from patients undergoing small intestine or liver transplantation.


RESULTS
This assay could detect MRP8/14 concentrations as low as 2 micro g/L. Within-run CVs were 3.7-6.1% and between-day CVs were 5.6-8.7% for MRP8/14 concentrations of 117-3300 micro g/L. Mean recovery was 104% (range, 80-128%). We observed a marked increase in serum MRP8/14 postoperatively in most recipients of transplants, followed by an increase in CRP 1-7 days after the increase in the complex. The increase in serum MRP8/14 occurred simultaneously with permeation of lymphocytes into the transplanted tissues as a result of rejection of the graft tissues.


CONCLUSIONS
Accurate measurement of serum MRP8/14 provides a useful clinical diagnostic method tool for detecting inflammation associated with rejection of transplanted tissues.
Ikemoto
Masaki
M
College of Medical Technology, Kyoto University, Japan. mmas@kuhp.kyoto-u.ac.jp
Tanaka
Tomoko
T
Takai
Yuri
Y
Murayama
Hiroshi
H
Tanaka
Koichi
K
Fujita
Masatoshi
M
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015415:Biomarkers / Q000097:blood; D040501:Calgranulin A / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis*; D006801:Humans; D007249:Inflammation / Q000175:diagnosis; D007421:Intestine, Small / Q000637:transplantation; D016031:Liver Transplantation; D008297:Male; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D040501:Calgranulin A