Central distribution of neuronal cell bodies innervating the levator veli palatini muscle and associated pattern of myosin heavy chain isoform expression in rat.
The levator veli palatini (LVP) is a muscle that plays a very important role in the complex functions regulating velopharyngeal function. Although previous studies have indicated that the contraction properties of the LVP closely resemble those of the intrinsic laryngeal muscle, histological evidence has not yet been obtained. The LVP is generally considered to be innervated by the glossopharyngeal nerve, which contains efferent and afferent components. LVP motoneurons are localized in the nucleus ambiguus (Amb), and afferent neurons project into the bilateral regions of the nucleus of the solitary tract (NST). However, the position of neuronal cell bodies on afferent neurons has remained unknown. The present study examined serial muscle cross-sections using monoclonal antibodies specific for myosin heavy chain (MyHC), to characterize muscle fibers of the LVP, clarify the central distribution of LVP motoneurons within the Amb and afferent terminals within the NST, and elucidate the location of LVP afferent neuronal cell bodies. Clear separation was observed within the LVP between fibers containing only fast MyHC and others positive for both slow and fast MyHC. Horseradish peroxidase (HRP)-labeled motoneurons in the Amb were separated into rostral and caudal divisions, corresponding to the Bötzinger complex and the rostral ventral respiratory group, respectively. HRP-labeled afferent neuronal cell bodies were observed in a glossopharyngo-vagal complex ganglion, and HRP-labeled afferent terminals were observed in bilateral lateral regions of the NST. These results suggest a relationship between MyHC isoform expression and the central distribution of LVP motoneurons or central projections of afferent neurons, with regard to activity of the LVP during both inspiration and expiration.
Yasuda
Kouichi
K
Department of Oral and Maxillofacial Surgery, Matsumoto Dental University School of Dentistry, Nagano 399-0781, Shiojiri, Japan.
Okuda
Daizo
D
Tanaka
Mikiko
M
Mori
Ryota
R
Hasumi-Nakayama
Yoko
Y
Tanaka
Mizuho
M
Yamaoka
Minoru
M
Furusawa
Kiyofumi
K
D000818:Animals; D007839:Functional Laterality; D005930:Glossopharyngeal Nerve / Q000033:anatomy & histology; D007150:Immunohistochemistry; D008526:Medulla Oblongata / Q000033:anatomy & histology / Q000378:metabolism; D009046:Motor Neurons / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000145:classification / Q000378:metabolism*; D009434:Neural Pathways / Q000033:anatomy & histology*; D010156:Palatal Muscles / Q000294:innervation*; D010160:Palate, Soft / Q000294:innervation; D020033:Protein Isoforms / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017552:Solitary Nucleus / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism; D014630:Vagus Nerve / Q000033:anatomy & histology; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate / Q000378:metabolism
D020033:Protein Isoforms; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D018995:Myosin Heavy Chains