Direct detection of dermatophytes in skin samples based on sequences of the chitin synthase 1 (CHS1) gene.
For the direct detection of dermatophytes in skin scrapings and hairs from animals, a primer pair specific to the chitin synthase 1 (CHS1) gene of dermatophytes was constructed. By PCR analysis with the primer pair, dermatophyte DNA could be diagnosed directly and rapidly in clinical skin samples.
Kano
Rui
R
Department of Pathobiology, Nihon University School of Veterinary Medicine, Kanagawa, Japan.
Hirai
Asuka
A
Muramatsu
Masato
M
Watari
Toshihiro
T
Hasegawa
Atsuhiko
A
D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000175:diagnosis* / Q000382:microbiology; D002415:Cats; D002687:Chitin Synthase / Q000235:genetics*; D003881:Dermatomycoses / Q000175:diagnosis* / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis* / Q000382:microbiology; D004285:Dogs; D005260:Female; D006197:Hair / Q000382:microbiology; D008297:Male; D008864:Microsporum / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011817:Rabbits; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017385:Sequence Homology; D012867:Skin / Q000382:microbiology; D014249:Trichophyton / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification
D002687:Chitin Synthase