Elevation of transgene expression level by flanking matrix attachment regions (MAR) is promoter dependent: a study of the interactions of six promoters with the RB7 3' MAR.
We have analyzed effects of a matrix attachment region (MAR) from the tobacco RB7 gene on transgene expression from six different promoters in stably transformed tobacco cell cultures. The presence of MARs flanking the transgene increased expression of constructs based on the constitutive CaMV 35S, NOS, and OCS promoters. Expression from an induced heat shock promoter was also increased and MARs did not cause expression in the absence of heat shock. There was also no effect of MARs on the pea ferredoxin promoter, which is not normally expressed in this cell line. Importantly, most transgenes flanked by RB7 MAR elements showed a large reduction in the number of low expressing GUS transformants relative to control constructs without MARs.
Mankin
S Luke
SL
Department of Botany, NC State University, Raleigh, NC 27695-7612, USA. mankins@basf.com
Allen
George C
GC
Phelan
Thomas
T
Spiker
Steven
S
Thompson
William F
WF
D020413:3' Untranslated Regions / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000502:physiology*; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006358:Hot Temperature; D015530:Nuclear Matrix / Q000502:physiology*; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics / Q000254:growth & development; D014170:Transformation, Genetic; D019076:Transgenes / Q000502:physiology*; D015854:Up-Regulation
D020413:3' Untranslated Regions; D017931:DNA Primers; D005966:Glucuronidase