title: Structure of the main ganglioside from the brain of Xenopus laevis.
abstract: The main component of the ganglioside(1) mixture from the brain of the adult amphibian Xenopus laevis accounts for 35% of the total, as lipid bound sialic acid. This ganglioside has been purified and characterized by thin layer chromatography, partial and exhaustive enzymatic hydrolysis with sialidase, TLC-overlay procedures with anti-Gg(4)Cer and anti-Neu5Acalpha6GalNAcbeta specific monoclonal antibodies and mass spectrometry. All together the results suggest the following structure: Neu5Acalpha8Neu5Acalpha3Galbeta3(Neu5Acalpha8Neu5Acalpha6)GalNAcbeta4Galbeta4Glcbeta1Cer, or, IV(3)-alpha-Neu5Ac(2),III(6)-alpha-Neu5Ac(2)-Gg(4)Cer.
authors.affiliation: Institute of General Physiology and Biochemistry, 20134 Milan, Italy.
authors.forename: Angela Maria
authors.initials: AM
authors.lastname: Rizzo
authors.forename: Bruno
authors.initials: B
authors.lastname: Berra
authors.forename: Federica
authors.initials: F
authors.lastname: Rossi
authors.forename: Anna
authors.initials: A
authors.lastname: Guerra
authors.forename: Rosalba
authors.initials: R
authors.lastname: Gornati
authors.forename: Giovanni
authors.initials: G
authors.lastname: Bernardini
authors.forename: Takao
authors.initials: T
authors.lastname: Taki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kasama
authors.forename: Laura
authors.initials: L
authors.lastname: Mauri
authors.forename: Sandro
authors.initials: S
authors.lastname: Sonnino
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: D005732:Gangliosides; D009439:Neuraminidase