Interplay of the Arabidopsis nonhost resistance gene NHO1 with bacterial virulence.
It is poorly understood why a particular plant species is resistant to the vast majority of potential pathogens that infect other plant species, a phenomenon referred to as "nonhost" resistance. Here, we show that Arabidopsis NHO1, encoding a glycerol kinase, is required for resistance to and induced by Pseudomonas syringae isolates from bean and tobacco. NHO1 is also required for resistance to the fungal pathogen Botrytis cinerea, indicating that NHO1 is not limited to bacterial resistance. Strikingly, P. s. pv. tomato DC3000, an isolate fully virulent on Arabidopsis, actively suppressed the NHO1 expression. This suppression is abolished in coi1 plants, indicating that DC3000 required an intact jasmonic acid signaling pathway in the plant to suppress NHO1 expression. Constitutive overexpression of NHO1 led to enhanced resistance to this otherwise virulent bacterium. The presence of avrB in DC3000, which activates a cultivar-specific "gene-for-gene" resistance in Arabidopsis, restored the induction of NHO1 expression. Thus, NHO1 is deployed for both general and specific resistance in Arabidopsis and targeted by the bacterium for parasitism.
Kang
Li
L
Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA.
Li
Jianxiong
J
Zhao
Tiehan
T
Xiao
Fangming
F
Tang
Xiaoyan
X
Thilmony
Roger
R
He
ShengYang
S
Zhou
Jian-Min
JM
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology*; D001483:Base Sequence; D020171:Botrytis / Q000472:pathogenicity; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D005816:Genetic Complementation Test; D005991:Glycerol Kinase / Q000235:genetics; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D011549:Pseudomonas / Q000472:pathogenicity*; D017386:Sequence Homology, Amino Acid; D014774:Virulence
D018744:DNA, Plant; D005991:Glycerol Kinase