title: The hemolytic and physiological activities of mixtures of some phenoxy and organophosphorous herbicides.
abstract: Experiments were performed investigating the potential to improve the biological activity of some phenoxy and organophosphorous compounds by using them in binary mixtures. The compounds were: 2,4-dichlorophenoxyacetic acid (1) and its sodium salt (2), dibutyl 1-butylamino-1-cyclohexanephosphonate (3) and diethyl 9-butylamino-9-fluorenephosphonate (4), all widely used as herbicides. There were two test methods: the inhibition of cucumber (Cucumis sativus) growth induced by one single herbicide or by equimolar binary mixtures of herbicides; and, in parallel, the hemolytic efficiency of separate compounds or their mixtures. The hemolytic properties of the compounds were studied as hemolysis is generally a good measure of their toxicity, especially in the case of lipophilic compounds. Pig erythrocytes were used as good models for the determination of toxicity and the kinetics of red blood cell hemolysis. In the plant-based experiments, binary mixtures were found to display additive type toxicity. The compounds' hemolytic activities were of additive or antagonistic types. In some combinations, the addition of a second component did not change the hemolytic efficiency of the first component, and vice versa.
authors.affiliation: Department of Physics and Biophysics, Agricultural University, Norwida 25, 50-375 Wrocław, Poland.
authors.forename: Halina
authors.initials: H
authors.lastname: Kleszczyńska
authors.forename: Dorota
authors.initials: D
authors.lastname: Bonarska
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Bielecki
authors.forename: Janusz
authors.initials: J
authors.lastname: Sarapuk
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000633:toxicity; D000818:Animals; D018553:Cucumis sativus / Q000187:drug effects / Q000254:growth & development; D004347:Drug Interactions; D004912:Erythrocytes / Q000187:drug effects; D005449:Fluorenes / Q000633:toxicity; D006461:Hemolysis / Q000187:drug effects*; D006540:Herbicides / Q000737:chemistry / Q000633:toxicity*; D066298:In Vitro Techniques; D063065:Organophosphonates / Q000633:toxicity; D009943:Organophosphorus Compounds / Q000633:toxicity; D034421:Sus scrofa
chemical_list: D005449:Fluorenes; D006540:Herbicides; D063065:Organophosphonates; D009943:Organophosphorus Compounds; C465763:diethyl 9-butylamino-9-fluorenephosphonate; D015084:2,4-Dichlorophenoxyacetic Acid; C078045:buminafos