Gelatinase A (MMP-2), collagenase-2 (MMP-8), and laminin-5 gamma2-chain expression in murine inflammatory bowel disease (ulcerative colitis).
Dextran sulfate sodium-induced inflammatory bowel disease in mice resembles human ulcerative colitis. In inflammatory bowel diseases matrix metalloproteinases contribute to tissue degradation. Laminin-5 is an anchoring filament protein in the basement membrane area that can be cleaved by matrix metalloproteinases. We investigated the expression of matrix metalloproteinases-2 and -8 and laminin-5 gamma2-chain in dextran sulfate sodium-induced mice by immunohistochemistry and in situ hybridization. Matrix metalloproteinase-8 expression was evidenced in the colon surface epithelial cells and the protein was more abundant in dextran sulfate sodium-induced mice colon. Matrix metallproteinase-2 and laminin-5 gamma2-chain colocalized in the colon surface epithelial cells and in the basement membrane zone as demonstrated by double immunostaining. In dextran sulfate sodium-induced colon, matrix metalloproteinase-2 immunoreactivity was detected in epithelial cells in the lower parts of the crypt and surrounding the degraded crypts. Matrix metalloproteinase-2 and -8 could participate in the local epithelial inflammatory processes and tissue destruction. The presence of laminin-5 gamma2-chain indicates alternative anchoring mechanisms in the colon, a compartment devoid of hemidesmosomes.
Pirilä
Emma
E
Department of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Biomedicum Helsinki, Finland.
Ramamurthy
Nungavarm S
NS
Sorsa
Timo
T
Salo
Tuula
T
Hietanen
Jarkko
J
Maisi
Päivi
P
D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000378:metabolism*; D003106:Colon / Q000378:metabolism*; D016264:Dextran Sulfate; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020784:Matrix Metalloproteinase 8 / Q000378:metabolism*; D051379:Mice; D000097794:Kalinin
D015815:Cell Adhesion Molecules; D016264:Dextran Sulfate; D020778:Matrix Metalloproteinase 2; D020784:Matrix Metalloproteinase 8; D000097794:Kalinin