title: Reconstruction of the muscle system in Antygomonas sp. (Kinorhyncha, Cyclorhagida) by means of phalloidin labeling and cLSM.
abstract: In the present investigation the entire muscle system of the cyclorhagid kinorhynch Antygomonas sp. was three-dimensionally reconstructed from whole mounts by means of FITC-phalloidin labeling and confocal scanning microscopy. With this technique, which proved to be especially useful for microscopically small species, we wanted to reinvestigate and supplement the findings obtained by histological and electron microscopical methods. The organization of the major muscle systems can be summarized as follows: 1) All muscle fibers, apart from the intestinal ones, the spine, and the mouth cone muscles, show a cross-striated pattern; 2) Dorsal longitudinal muscle fibers as well as segmentally arranged dorsoventral fibers occur from segment III to XIII; 3) Diagonal muscle fibers are located laterally in segments III to X; 4) Two rings of circular fibers are present in segment II, forming the closing apparatus in Cyclorhagida. Further circular muscles are present in segment I, forming the mouth cone and the eversible introvert, and in the pharyngeal bulb.
authors.affiliation: Spezielle Zoologie, Fachbereich 5, Universität Osnabrück, D-49069 Osnabrück, Germany. MCMueller@biologie.uni-osnabrueck.de
authors.forename: Monika C M
authors.initials: MC
authors.lastname: Müller
authors.forename: Andreas
authors.initials: A
authors.lastname: Schmidt-Rhaesa
mesh_terms: D000818:Animals; D007448:Invertebrates / Q000033:anatomy & histology*; D018613:Microscopy, Confocal; D009132:Muscles / Q000033:anatomy & histology*; D010590:Phalloidine; D013194:Staining and Labeling
chemical_list: D010590:Phalloidine