title: [Standardization of neutralization tests using the COBL cell line and comparison with the particle agglutination test for measles serology].
abstract: The aim of the present study was the detection and comparison of measles antibody titers with particle agglutination (PA) and neutralization (Nt) methods, in the sera samples of 364 subjects from different age groups. PA method was performed with a commercial test kit (Serodiameasles, Fujirebio Com. Japan), and Nt test which was standardized in this study, by using COBL (cord blood) cell lines, has been started to use in our laboratory as a reference method. As a result, antibody titers detected by PA were in parallel to the titers which detected by Nt test, and it was concluded that the differences in antibody titers would arise from the differences of test principles and viral antigens.
authors.affiliation: Refik Saydam Hifzissihha Merkezi, Viroloji Laboratuvari, Ankara.
authors.forename: Gülay
authors.initials: G
authors.lastname: Korukluoğlu
authors.forename: Tülay
authors.initials: T
authors.lastname: Yalçinkaya
authors.forename: Etem
authors.initials: E
authors.lastname: Ozkaya
authors.forename: Demet
authors.initials: D
authors.lastname: Kurtoğlu
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Gözalan
authors.forename: Kikuko
authors.initials: K
authors.lastname: Miyamura
mesh_terms: D000293:Adolescent; D000328:Adult; D000372:Agglutination Tests / Q000592:standards*; D000914:Antibodies, Viral / Q000097:blood*; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008457:Measles / Q000175:diagnosis* / Q000276:immunology; D008459:Measles virus / Q000276:immunology*; D009500:Neutralization Tests / Q000592:standards*; D011933:Reagent Kits, Diagnostic
chemical_list: D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic