PARP-3 localizes preferentially to the daughter centriole and interferes with the G1/S cell cycle progression.
A novel member of the poly(ADP-ribose) polymerase (PARP) family, hPARP-3, is identified here as a core component of the centrosome. hPARP-3 is preferentially localized to the daughter centriole throughout the cell cycle. The N-terminal domain (54 amino acids) of hPARP-3 is responsible for its centrosomal localization. Full-length hPAPR-3 (540 amino acids, with an apparent mass of 67 kDa) synthesizes ADP-ribose polymers during its automodification. Overexpression of hPARP-3 or its N-terminal domain does not influence centrosomal duplication or amplification but interferes with the G1/S cell cycle progression. PARP-1 also resides for part of the cell cycle in the centrosome and interacts with hPARP-3. The presence of both PARP-1 and PARP-3 at the centrosome may link the DNA damage surveillance network to the mitotic fidelity checkpoint.
Augustin
Angélique
A
Unité 9003 du CNRS, Ecole Supérieure de Biotechnologie de Strasbourg, Boulevard Sébastien Brant, 67400 Illkirch, France.
Spenlehauer
Catherine
C
Dumond
Hélène
H
Ménissier-De Murcia
Josiane
J
Piel
Matthieu
M
Schmit
Anne-Catherine
AC
Apiou
Françoise
F
Vonesch
Jean-Luc
JL
Kock
Michael
M
Bornens
Michel
M
De Murcia
Gilbert
G
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D002502:Centrioles / Q000378:metabolism*; D006224:Cricetinae; D016193:G1 Phase / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D051379:Mice; D008969:Molecular Sequence Data; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism*; D016196:S Phase / Q000187:drug effects; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D018797:Cell Cycle Proteins; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C480979:PARP3 protein, human; D011065:Poly(ADP-ribose) Polymerases; D006918:Hydroxyurea