title: [Disturbed cooperation of hepatocytes in the rhythm of protein synthesis by chelating agent of cytoplasmic calcium BAPTA-AM].
abstract: Previously we demonstrated synchronized oscillations of protein synthesis rate in the hepatocyte cultures upon accumulation of monosialognaglioside GM1 in the medium or after introduction of exogenous GM1 to the medium. The synchronized oscillations of the protein synthesis rate in dense hepatocyte cultures were blocked 30 min after their treatment with 10-20 microM BAPTA-AM--a chelating agent of cytoplasmic calcium. Enzyme immunoassay for GM1 demonstrated similar amounts of GM1 in the medium conditioned for 3 h by dense hepatocyte cultures pretreated with 20 microns]M BAPTA-AM for 1 h and in the medium of normal dense cultures--0.0060 +/- 0.0005 and 0.0055 +/- 0.0005 pmol/1000 cells, respectively. The content of GM1 was also similar in the normal and BAPTA-AM-pretreated hepatocytes--0.158 +/- 0.013 and 0.183 +/- 0.014 pmol/1000 cells, respectively. The synchronized rhythm of protein synthesis has been confirmed in the diluted cultures in the medium conditioned by the normal dense cultures. However, the medium conditioned by the dense cultures pretreated with BAPTA-AM induces no synchronization of the diluted cultures. Since GM1 concentration was normal in this medium, we propose the effect of physicochemical form of the gangliosides accumulated in the medium on their ability to synchronize the rhythm of protein synthesis.
authors.affiliation: Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia.
authors.forename: N D
authors.initials: ND
authors.lastname: Zvesdina
authors.forename: N V
authors.initials: NV
authors.lastname: Nechaeva
authors.forename: E V
authors.initials: EV
authors.lastname: Gracheva
authors.forename: T E
authors.initials: TE
authors.lastname: Novikova
authors.forename: I G
authors.initials: IG
authors.lastname: Gvazava
authors.forename: V I
authors.initials: VI
authors.lastname: Fateeva
authors.forename: L A
authors.initials: LA
authors.lastname: Mal'chenko
authors.forename: V Ia
authors.initials: VIa
authors.lastname: Brodskiĭ
mesh_terms: D000818:Animals; D001683:Biological Clocks / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology*; D017077:Culture Media, Conditioned / Q000378:metabolism / Q000494:pharmacology; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D004533:Egtazic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005677:G(M1) Ganglioside / Q000378:metabolism / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000187:drug effects; D051381:Rats
chemical_list: D002614:Chelating Agents; D017077:Culture Media, Conditioned; D011506:Proteins; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; D005677:G(M1) Ganglioside; D004533:Egtazic Acid; D002118:Calcium