title: Role of bradykinin in angiotensin-converting enzyme knockout mice.
abstract: Angiotensin-converting enzyme (ACE) plays a central role in the renin-angiotensin system. Whereas ACE is responsible for the production of angiotensin II, it is also important in the elimination of bradykinin. Constitutively, the biological function of bradykinin is mediated through the bradykinin B(2) receptor. ACE knockout mice have a complicated phenotype including very low blood pressure. To investigate the role of bradykinin in the expression of the ACE knockout phenotype, we bred B(2) receptor knockout mice with ACE knockout mice, thus generating a line of mice deficient in both the B(2) receptor and ACE. Surprisingly, these mice did not differ from ACE knockout mice in blood pressure, urine concentrating ability, renal pathology, and hematocrit. Thus abnormalities of bradykinin accumulation do not play an important role in the ACE knockout phenotype. Rather, this phenotype appears due to the defective production of angiotensin II.
authors.affiliation: Department of Pathology, Emory University, Atlanta, Georgia 30322, USA.
authors.forename: Hong D
authors.initials: HD
authors.lastname: Xiao
authors.forename: Sebastien
authors.initials: S
authors.lastname: Fuchs
authors.forename: Justin M
authors.initials: JM
authors.lastname: Cole
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Disher
authors.forename: Roy L
authors.initials: RL
authors.lastname: Sutliff
authors.forename: Kenneth E
authors.initials: KE
authors.lastname: Bernstein
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000235:genetics; D001920:Bradykinin / Q000494:pharmacology / Q000502:physiology*; D018076:DNA, Complementary / Q000235:genetics; D005838:Genotype; D006400:Hematocrit; D007668:Kidney / Q000473:pathology; D007671:Kidney Concentrating Ability / Q000235:genetics; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009206:Myocardium / Q000473:pathology; D009929:Organ Size / Q000502:physiology; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D010641:Phenotype; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin; D007703:Peptidyl-Dipeptidase A; D001920:Bradykinin