Effects of a low extracellular magnesium concentration and endotoxin on IL-1beta and TNF-alpha release from, and mRNA levels in, isolated rat alveolar macrophages.
The aim of this study was to assess the effects of a low extracellular Mg2+ concentration and endotoxin on interleukin (IL)-1beta and tumour necrosis factor (TNF)-alpha release from, and mRNA levels in, rat alveolar macrophages. A low extracellular Mg2+ concentration enhanced the release of both cytokines, and this release was suppressed by a calcium antagonist, nifedipine. Bacterial lipopolysaccharide (LPS) also enhanced the release of both cytokines, and this enhancement was stronger in a low-Mg2+ medium than in control medium. Furthermore, LPS increased the mRNA levels of both cytokines in alveolar macrophages, and this increase was enhanced in low-Mg2+ medium. These results suggest that a low extracellular Mg2+ concentration and LPS stimulate the release of IL-1beta and TNF-alpha from rat alveolar macrophages by increasing the synthesis of both cytokines and by Ca2+ signaling pathways.
Shogi
Toyokazu
T
Department of Veterinary Pharmacology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Oono
Hiromi
H
Nakagawa
Masae
M
Miyamoto
Atsushi
A
Ishiguro
Shigeru
S
Nishio
Akira
A
D000818:Animals; D003470:Culture Media; D017931:DNA Primers; D004731:Endotoxins / Q000494:pharmacology*; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis*; D016676:Macrophages, Alveolar / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D003470:Culture Media; D017931:DNA Primers; D004731:Endotoxins; D007375:Interleukin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C044101:endotoxin, Escherichia coli; D008274:Magnesium