Enhanced induction of hepatitis C virus-specific cytotoxic T lymphocytes and protective efficacy in mice by DNA vaccination followed by adenovirus boosting in combination with the interleukin-12 expression plasmid.
We evaluated the prime-boost immunization consisting of hepatitis C virus (HCV)-core expression plasmid (pCEP4-core) and replication-defective adenovirus expressing HCV-core (Adex1SR3ST) for core-specific CTL induction in mice. Compared to a single booster, double boosters after priming enhance CTL induction. The prime-double boosts immunization involving pCEP4-core priming followed by pCEP4-core and Adex1SR3ST boostings (pC/pC/aC) can induce core-specific CTLs as well as other combinations: pC/aC/aC; aC/pC/pC; aC/aC/aC, whereas pC/pC/pC does not induce CTLs. Furthermore, co-administration of interleukin-12 (IL-12) expression plasmid leads to the highly efficient CTL induction and clearance of HCV-core expressing vaccinia virus challenged. Thus, the prime-double boosts immunization together with IL-12 may be promising for HCV vaccine.
Matsui
Masanori
M
Department of Microbiology, Saitama Medical School, Moroyama-Cho, Iruma-Gun, 350-0495, Saitama, Japan. matsui@saitama-med.ac.jp
Moriya
Osamu
O
Akatsuka
Toshitaka
T
D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004359:Drug Therapy, Combination; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D016174:Hepacivirus / Q000276:immunology*; D006801:Humans; D007117:Immunization, Secondary / Q000379:methods*; D018664:Interleukin-12 / Q000008:administration & dosage* / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000096:biosynthesis / Q000494:pharmacology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000821:virology*; D014407:Tumor Cells, Cultured; D019444:Vaccines, DNA / Q000276:immunology*
D018984:Epitopes, T-Lymphocyte; D019444:Vaccines, DNA; D018664:Interleukin-12