Functional post-translational proteomics approach to study the role of N-glycans in the development of Caenorhabditis elegans.
Glycosylation is one of the most common post-translational protein modifications. Carbohydrate-mediated interactions between cells and their environment are important in differentiation, embryogenesis, inflammation, cancer and metastasis and other processes. Humans and mice with mutations that prevent normal N-glycosylation show multi-systemic defects in embryogenesis, thereby proving that these molecules are essential for normal development; however, a large number of proteins undergo defective glycosylation in these human and mouse mutants, and it is therefore difficult to determine the precise molecular roles of specific N-glycans on individual proteins. We describe here a 'functional post-translational proteomics' approach that is designed to determine the role of N-glycans on individual glycoproteins in the development of Caenorhabditis elegans.
Schachter
Harry
H
Department of Structural Biology and Biochemistry, Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada. harry@sickkids.on.ca
Chen
Shihao
S
Zhang
Wenli
W
Spence
Andrew M
AM
Zhu
Shaoxian
S
Callahan
John W
JW
Mahuran
Don J
DJ
Fan
Xiaolian
X
Bagshaw
Rick D
RD
She
Yi-Min
YM
Rosa
J Cesar
JC
Reinhold
Vernon N
VN
D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry / Q000254:growth & development*; D013058:Mass Spectrometry; D011134:Polysaccharides / Q000737:chemistry / Q000502:physiology*; D011499:Protein Processing, Post-Translational; D040901:Proteomics
D011134:Polysaccharides