[Aspiration biopsy RNA diagnosis].
Molecular-based diagnosis of carcinomas can be more easily established by utilizing specific mRNAs that are restrictively expressed in cancer tissues. Recently a new method of preoperative molecular-based diagnosis of thyroid carcinomas was established. This technique, Aspiration Biopsy RNA Diagnosis, ABRD, allows us to perform preoperative RNA analyses by extracting RNAs from tumor cells obtained by fine needle aspiration biopsies(FNABs). By ABRD detecting of oncofetal fibronectin or calcitonin mRNA in FNABs, papillary, anaplastic and medullary carcinomas are accurately diagnosed preoperatively. Further, by real-time monitoring RT-PCR, a fully automated system was established.
Takano
Toru
T
Department of Laboratory Medicine, Osaka University Graduate School of Medicine.
Amino
Nobuyuki
N
D014408:Biomarkers, Tumor / Q000032:analysis*; D001707:Biopsy, Needle / Q000379:methods*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013964:Thyroid Neoplasms / Q000175:diagnosis*
D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D012334:RNA, Neoplasm