title: Effect of recombinant basic fibroblast growth factor (bFGF) on fibroblast-like cells from human rotator cuff tendon.
abstract: Rotator cuff tendon cells (RCC) derived from surgical samples showed fibroblast-like morphology. Histological staining demonstrated collagen secretion by RCC. Immunohistological findings revealed that RCC secreted type I and III collagen, but not type II collagen. In addition, the SDS-PAGE analysis suggested that RCC predominantly produced type I collagen. Basic fibroblast growth factor (bFGF) had a stimulatory effect on the proliferation of RCC dose-dependently up to 1 ng/ml. Administration of bFGF suppressed the secretion of collagens from RCC in a dose-dependent manner.
authors.affiliation: Department of Orthopedic Surgery, Akita University School of Medicine, Akita 010-8543, Japan. shu@med.akita-u.ac.jp
authors.forename: Shu
authors.initials: S
authors.lastname: Takahashi
authors.forename: Mizuho
authors.initials: M
authors.lastname: Nakajima
authors.forename: Moto
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Ikuko
authors.initials: I
authors.lastname: Wakabayashi
authors.forename: Naohisa
authors.initials: N
authors.lastname: Miyakoshi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Minagawa
authors.forename: Eiji
authors.initials: E
authors.lastname: Itoi
mesh_terms: D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D017006:Rotator Cuff / Q000187:drug effects* / Q000378:metabolism; D013710:Tendons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D013997:Time Factors
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D013778:Tetrazolium Salts; D013844:Thiazoles; D016222:Fibroblast Growth Factor 2; C022616:thiazolyl blue