title: Activation of influenza virus RNA polymerase by the 5' and 3' terminal duplex of genomic RNA.
abstract: The current model for influenza virus mRNA transcription involves the sequential interaction of the viral polymerase with the 5'- and 3'-ends of vRNA, with each RNA-protein interaction triggering a polymerase function necessary for cap-primed transcription. Here we show that the order in which this ternary complex is assembled is in fact important. Polymerase bound simultaneously to a pre-annealed duplex of the 5'- and 3'-ends of vRNA had greatly increased levels of primer binding and endonuclease activities compared to a sequentially assembled complex. Increased primer binding was due to the activation of a high affinity binding site with a preference for primer length RNAs. This correlated with enhanced levels of cap-primed transcription. Polymerase that was bound initially to just 5' vRNA had low primer binding activity, but was endonucleolytically active. Neither activity was significantly increased by the subsequent addition of 3' vRNA, and this sequentially assembled complex had correspondingly low mRNA transcription activity. Nevertheless, both routes of assembly led to complexes that were highly competent for dinucleotide ApG-primed transcription. Therefore, polymerase complexes assembled on pre-annealed 5' and 3' terminal viral RNA sequences have distinct properties from those assembled by sequential loading of polymerase onto the 5'-end followed by the 3'-end. This suggests a mechanism by which the virus couples transcription initiation and termination during mRNA transcription.
authors.affiliation: Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK.
authors.forename: M-T Michael
authors.initials: MT
authors.lastname: Lee
authors.forename: Klaus
authors.initials: K
authors.lastname: Klumpp
authors.forename: Paul
authors.initials: P
authors.lastname: Digard
authors.forename: Laurence
authors.initials: L
authors.lastname: Tiley
mesh_terms: D004720:Endonucleases / Q000378:metabolism; D004789:Enzyme Activation; D016679:Genome, Viral; D007700:Kinetics; D011485:Protein Binding; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C061290:PA protein, influenza viruses; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase; D004720:Endonucleases