title: [Preparation and application of the monoclonal antibody against hepatoma-specific gamma-glutamyltransferase].
abstract: OBJECTIVE
  To prepare a monoclonal antibody against hepatoma-specific gamma-glutamyltransferase (GGT-II) and study it's application.
  
  
  METHODS
  Two Bal B/C mice were immunized with pure GGT-II, then their spleen cells were separated and fused to SP 2/0 myeloma cells so as to make hybridoma cell strain which could yield monoclonal antibody against GGT-II. And it's effect of binding GGT-II was detected by competitive inhibitory enzyme linked-immunosorbance assay (ELISA).
  
  
  RESULTS
  A mouse hybridoma cell strain which could steadily secrete the monoclonal antibody against GGT-II was obtained and named 2G4F6B2. This monoclonal antibody belonged to IgG1 subclass and was specific to GGT-II, without cross-reaction to GGT-II. The result of detecting human serum GGT-II by ELISA with the monoclonal antibody accorded with that by polyacrylamide gradient electrophoresis.
  
  
  CONCLUSIONS
  The monoclonal antibody against GGT-II prepared in this study has high specificity and can be applied in clinic to detect human serum GGT-II.
authors.affiliation: Department of Infectious Disease, Affiliated Hospital of Nantong Medical College, Nantong 226001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Xiao Ying
authors.initials: XY
authors.lastname: Wang
authors.forename: Jian Ee
authors.initials: JE
authors.lastname: Gao
authors.forename: He Ping
authors.initials: HP
authors.lastname: Ji
authors.forename: Qi Min
authors.initials: QM
authors.lastname: Tao
authors.forename: Ying
authors.initials: Y
authors.lastname: Ji
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000201:enzymology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008113:Liver Neoplasms / Q000175:diagnosis / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D005723:gamma-Glutamyltransferase / Q000032:analysis / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D005723:gamma-Glutamyltransferase