A role of the Duffy antigen for the maintenance of plasma chemokine concentrations.
We examined plasma chemokine concentrations and chemokine clearance rates in Duffy antigen knockout mice. The plasma concentrations of eotaxin and MCP-1 in Duffy antigen knockout mice were less than one-third of those in wild-type mice. When eotaxin or hMGSA was intravenously injected, the chemokine disappeared more rapidly from the plasma of Duffy antigen knockout mice than from the plasma of wild-type mice. The half-lives of hIP-10 and interferon-gamma, which do not have an affinity for the Duffy antigen, in plasma were indistinguishable between Duffy antigen knockout mice and wild-type mice. These results suggest that the Duffy antigen delays the disappearance of chemokines from the plasma, resulting in the maintenance of plasma chemokine concentrations.
Fukuma
Nobuko
N
Laboratory of Developmental Biochemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 3-1, 7-Chome, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Akimitsu
Nobuyoshi
N
Hamamoto
Hiroshi
H
Kusuhara
Hiroyuki
H
Sugiyama
Yuichi
Y
Sekimizu
Kazuhisa
K
D000818:Animals; D000953:Antigens, Protozoan; D002352:Carrier Proteins / Q000378:metabolism* / Q000502:physiology*; D054413:Chemokine CCL11; D054360:Chemokine CXCL1; D054357:Chemokine CXCL10; D018925:Chemokines / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics; D019742:Chemokines, CC / Q000097:blood; D019743:Chemokines, CXC / Q000378:metabolism; D002630:Chemotactic Factors / Q000493:pharmacokinetics; D004375:Duffy Blood-Group System / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins / Q000493:pharmacokinetics; D007371:Interferon-gamma / Q000493:pharmacokinetics; D051379:Mice; D018345:Mice, Knockout; D015800:Protozoan Proteins / Q000378:metabolism* / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000378:metabolism* / Q000502:physiology*; D013997:Time Factors
D000953:Antigens, Protozoan; D002352:Carrier Proteins; C515457:Ccl11 protein, mouse; D054413:Chemokine CCL11; D054360:Chemokine CXCL1; D054357:Chemokine CXCL10; D018925:Chemokines; D019742:Chemokines, CC; D019743:Chemokines, CXC; D002630:Chemotactic Factors; C475895:Cxcl1 protein, mouse; D004375:Duffy Blood-Group System; C066001:Duffy antigen binding protein, Plasmodium; D036341:Intercellular Signaling Peptides and Proteins; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; D007371:Interferon-gamma