[Effect of beta-interleukin on the content and proliferation of hemopoietic precursor cells in intact and irradiated long-term cultures of the bone marrow].
Effects of beta-leukine (human recombinant interleukin-1 beta) on the number and proliferative activity of early hemopoietic precursor cells (CFU-GM) and colonystimulating activity (GM-CSF) were studied in long-term cultures of the bone marrow. Studied were also recovery of CFU-GM, total number of myelocaryocytes and content of immature and mature granulocytes in response to beta-leukine after gamma-radiation of cultures in a dose 2.0 Gy. It was found that beta-leukine enhanced GM-CSF induction and elevated content of early CFU-GM in non-radiated long-term cultures of the bone marrow maximum 20 hours after introduction. 20 hours after introduction of beta-leukine proliferative activity of CFU-GM reached its maximum. The study of postradiation recovery in administration of beta-leukine in a dose 0.01 mcg/ml 10 min after radiation of long-term cultures of the bone marrow registered no significant stimulating effect of the drug on CFU-GN recovery, recovery of the number of myelocaryocytes and content of young immature and mature granulocytes.
Lebedev
V G
VG
Moroz
B B
BB
Deshevoĭ
Iu B
IuB
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000528:radiation effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005720:Gamma Rays; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000528:radiation effects; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011829:Radiation Dosage; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors
D007375:Interleukin-1; D011837:Radiation-Protective Agents; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor