Intracellular targeting of calmodulin mRNAs in primary hippocampal cells.
We investigated the intracellular distribution of the mRNAs corresponding to the three non-allelic CaM genes in cultured hippocampal cells by in situ hybridization with digoxigenin-labeled gene-specific riboprobes. In neurons the perikaryon was heavily stained and strong dendritic mRNA targeting was detected for all three CaM genes. The color labeling exhibited a punctate distribution, suggesting that CaM mRNAs are transported in RNA granules. Immunocytochemistry for S100 demonstrated that glial cells express CaM mRNAs at a very low level. A minority of the cultured cells were negative for either labeling.
Kortvely
Elod
E
Department of Zoology and Cell Biology, University of Szeged, Szeged, Hungary.
Varszegi
Szilvia
S
Palfi
Arpad
A
Gulya
Karoly
K
D000818:Animals; D002147:Calmodulin / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats
D002147:Calmodulin; D012333:RNA, Messenger