title: Expression pattern of the cell cycle promoter cyclin e in benign extravillous trophoblast and gestational trophoblastic lesions: correlation with expression of Ki-67.
abstract: In this study, a specific monoclonal antibody was used to immunohistochemically investigate correlated expression of the cell cycle promoter cyclin E and the proliferation marker Ki-67 in benign extravillous trophoblast and gestational trophoblastic lesions. Our data show that cyclin E is expressed in the normal extravillous trophoblast, with strongest levels of expression in the cell columns of anchoring villi. Differences could be observed in expression of Ki-67 in both normal extravillous trophoblast and gestational trophoblastic lesions. In the extravillous trophoblast of the cell columns, expression of cyclin E started more distal compared with Ki-67 and was maintained (with less intensity) into the deeper layers of interstitial trophoblast. In the benign trophoblastic lesions (exaggerated placental site [EPS] and placental site nodule [PSN]) and in the trophoblast proliferations on the surface of hydropic villi of hydatidiform moles (HM), the percentage of cells expressing cyclin E was higher than of those expressing Ki-67. The same observation could be made for a case of placental site trophoblastic tumor (PSTT). In contrast, choriocarcinomas (N=8), which are definitely malignant tumors, showed an opposite pattern, with a much higher percentage of strongly Ki-67-positive cells compared with cyclin E-positive cells. We conclude that cyclin E is expressed in benign extravillous trophoblast and gestational trophoblastic lesions, where a ratio cyclin E/Ki-67<1 characterizes choriocarcinomas, whereas PSTT and the benign lesions (HM, EPS, PSN) show expression of cyclin E in a higher percentage of cells than Ki-67 (cyclin E/Ki-67 ratio >1).
authors.affiliation: Department of Gynecopathology, Institute of Pathology, University Hospital Eppendorf, Hamburg, Germany.
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Bamberger
authors.forename: Solveig
authors.initials: S
authors.lastname: Aupers
authors.forename: Karin
authors.initials: K
authors.lastname: Milde-Langosch
authors.forename: Thomas
authors.initials: T
authors.lastname: Löning
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D019927:Cyclin E / Q000096:biosynthesis*; D005260:Female; D015870:Gene Expression; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D011247:Pregnancy; D014328:Trophoblastic Neoplasms / Q000378:metabolism* / Q000473:pathology; D014327:Trophoblasts / Q000378:metabolism* / Q000473:pathology; D014594:Uterine Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D019927:Cyclin E; D019394:Ki-67 Antigen