Further studies on the structural analysis of the cuticle of Litomosoides chagasfilhoi (Nematoda: Filarioidea).
In order to obtain further information on the structural organization of the cuticle of nematodes, this structure was isolated from adult forms of the filariid Litomosoides chagasfilhoi. The purity of the fraction was determined by light and transmission electron microscopy, deep-etching, high resolution scanning electron microscopy, atomic force microscopy, immunocytochemistry, gel electrophoresis (SDS-PAGE) and Western blot. The epicuticle presented a rugous surface with parallel rows and several globular particles that could be involved in the absorption of nutrients and secretion of products. Analysis by SDS-PAGE of purified cuticles revealed five major polypeptides corresponding to 151, 41, 28, 13 and 11 kDa. A polyclonal antibody against a synthetic 18 amino-acid peptide that corresponds to the sequence of domain E of the Haemonchus contortus3A3 collagen gene recognized several protein bands on the Western blot of purified cuticle, and labeled all cuticular layers, as shown by immunocytochemistry.
de Moraes Neto
A H A
AH
Laboratório de Helmintologia Programa de Biologia Celular e Parasitologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, 21949-900, Rio de Janeiro, RJ, Brazil.
Lanfredi
R M
RM
Gadelha
C
C
Cunha-e-Silva
N L
NL
Simão
R A
RA
Achete
C
C
de Souza
W
W
D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D005370:Filarioidea / Q000737:chemistry / Q000145:classification / Q000166:cytology / Q000648:ultrastructure*; D005613:Freeze Etching / Q000379:methods; D007150:Immunohistochemistry; D018625:Microscopy, Atomic Force / Q000379:methods
D000947:Antigens, Helminth