Versatile inducible activation system of Akt/PKB signaling pathway in mice.
We report a transgenic mouse line in which Akt/protein kinase B (PKB) pathway can be activated in an inducible manner in defined cell types. In this transgenic mouse line, Cre expression allows the expression of a tamoxifen-activatable form of Akt/PKB in a defined cell type. Subsequent injection of tamoxifen triggers the transient activation of Akt/PKB in mice. Thus, this transgenic line allows the transient activation of Akt/PKB pathway in a predefined cell type. We expect that this transgenic system will provide a unique tool to study the roles of Akt/PKB pathway in mice.
Kroll
Jens
J
The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390-8573, USA.
Cobo
Patricia
P
Sato
Thomas N
TN
D000818:Animals; D004789:Enzyme Activation / Q000502:physiology; D017930:Genes, Reporter; D051379:Mice; D008822:Mice, Transgenic; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt; D015398:Signal Transduction / Q000502:physiology*; D013629:Tamoxifen / Q000378:metabolism
D011518:Proto-Oncogene Proteins; D013629:Tamoxifen; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt