Binding of antibromelain monomeric Fab' improves the stability of stem bromelain against inactivation.
Antienzyme polyclonal antibodies against stem bromelain were raised in male albino rabbits and the Fab' monomers isolated from the IgG of the immune sera. Incubation of bromelain with the Fab' resulted in binding and gel filtration of the resulting complex suggested a 1:1 stoichiometry. Complexing with the Fab' resulted in significant stabilization of bromelain against thermal inactivation and alkaline pH.
Gupta
Pawan
P
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Uttar Pradesh, India.
Khan
Rizwan H
RH
Saleemuddin
M
M
D000818:Animals; D000936:Antigen-Antibody Complex / Q000737:chemistry; D001963:Bromelains / Q000008:administration & dosage / Q000276:immunology*; D004795:Enzyme Stability / Q000276:immunology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007140:Immunoglobulin Fab Fragments / Q000276:immunology* / Q000302:isolation & purification; D008297:Male; D011817:Rabbits
D000936:Antigen-Antibody Complex; D007140:Immunoglobulin Fab Fragments; D001963:Bromelains; C091760:stem bromelain