title: A novel GlcNAcalpha1-HPO3-6Gal(1-1)ceramide antigen and alkylated inositol-phosphoglycerolipids expressed by the liver fluke Fasciola hepatica.
abstract: The acidic (glyco)lipids of the parasitic liver fluke Fasciola hepatica exhibited two different phosphate-containing species, designated AL-I and AL-II, which were analyzed by MALDI-TOF MS, ESI MS, NMR, methylation analysis, and combined GC-MS in conjunction with HF treatment. AL-I was structurally determined as 1-O-hexadecyl-sn-glycerol-3-phosphoinositol, an ether bond variant of lysophosphatidylinositol. The structure of AL-II was shown to be GlcNAcalpha1-HPO3-6Gal(1-1)ceramide. Ceramide analysis revealed as major components 2-hydroxyoctadecanoic acid [18:0(2-OH)] together with C18- and C20-phytosphingosines. AL-II was apparently highly antigenic and strongly recognized by both animal- and human-F. hepatica infection sera. Furthermore, inhibition ELISAs revealed that the unusual antigenic determinant GlcNAcalpha1-HPO3- phosphate might have a potential in the serodiagnosis of F. hepatica infections.
authors.affiliation: Institute of Biochemistry, Medical Faculty, University of Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Wuhrer
authors.forename: Christiane
authors.initials: C
authors.lastname: Grimm
authors.forename: Ulrich
authors.initials: U
authors.lastname: Zahringer
authors.forename: Roger D
authors.initials: RD
authors.lastname: Dennis
authors.forename: Clemens M
authors.initials: CM
authors.lastname: Berkefeld
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Idris
authors.forename: Rudolf
authors.initials: R
authors.lastname: Geyer
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000032:analysis / Q000737:chemistry; D002518:Ceramides / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005210:Fasciola hepatica / Q000737:chemistry* / Q000276:immunology; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D006858:Hydrofluoric Acid / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010716:Phosphatidylinositols / Q000032:analysis* / Q000737:chemistry; D012756:Sheep; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D002518:Ceramides; D010716:Phosphatidylinositols; D006858:Hydrofluoric Acid