Biomarkers of carcinogenic contaminants in Baltic flounder (Platichthys flesus): temporal changes in urban and non-urban sites in Tallinn bay.
In order to study effects of environmental contamination, a suite of biomarkers were measured over the period 1996 to 1999 in livers of flounder (Platichthys flesus) from two urban embayments and one non-urban reference site of the Gulf of Finland in the vicinity of Tallinn, Estonia. Total cytochrome P450 (CYP) level, aryl hydrocarbon hydroxylase (AHH), 5-aminolevulinic acid synthetase (ALA-S), and heme synthetase (HEM-S) activities were quantified by means of spectrophotometry. These data were compared to results obtained in 1994 for the same biomarkers at one of the urban embayments and the non-urban site, as measured by the same protocols. For the flounder collected from the non-urban site, changes occurred in AHH activity and the total CYP level, which were significantly lower in 1996 and 1999 compared with 1994 (p < 0.05). Activity of ALA-S decreased slightly over this same period. The activity of HEM-S increased between 1996 and 1999. In the urban site first investigated in 1994, the activities of AHH and ALA-S, as well as the total level of CYP in flounder liver were significantly higher compared with 1999 (p < 0.05). HEM-S activities did not show any significant changes over this time period. AHH activities of flounder collected in another urban site decreased slightly between 1996 and 1999, in contrast to data on the total CYP level which diminished drastically over these years (p < 0.05). Activities of HEM-S increased significantly (p < 0.05) during the period investigated, while activities of ALA-S remained unchanged. These findings suggest that contamination of the marine environments by PAHs has gone down everywhere in the Tallinn area during the last 3 to 5 years. However, the results indicate that the area is still contaminated, as indicated by elevated heme synthesis enzymes and the total CYP content, and that monitoring of contaminants and their effects should be continued in this region.
Bogovski
Sergei
S
Institute of Experimental and Clinical Medicine, Hiiu Str. 42, EE-11619 Tallinn, Estonia.
Muzyka
Vladimir
V
Sergeyev
Boris
B
Karlova
Svetlana
S
D000624:5-Aminolevulinate Synthetase / Q000032:analysis / Q000494:pharmacology*; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000032:analysis / Q000494:pharmacology*; D015415:Biomarkers / Q000032:analysis*; D002273:Carcinogens / Q000009:adverse effects / Q000032:analysis*; D002947:Cities; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000494:pharmacology*; D004781:Environmental Exposure; D005294:Ferrochelatase / Q000032:analysis / Q000494:pharmacology*; D005432:Flounder / Q000502:physiology*; D008099:Liver / Q000201:enzymology; D014874:Water Pollutants, Chemical / Q000009:adverse effects / Q000032:analysis*
D015415:Biomarkers; D002273:Carcinogens; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; D000624:5-Aminolevulinate Synthetase; D005294:Ferrochelatase