[Polymerase chain reaction for Chlamydia trachomatis in urine specimens from patients with mucopurulent genital discharge].
The aim of this study was to investigate the DNA of Chlamydia trachomatis by polymerase chain reaction (PCR) in the first-void urine samples of patients with mucopurulent genital discharge and to compare the results with the urethral/endocervical swab culture method. First-void urine samples from 56 patients (46 female, 14 male) and urethral swab samples from 14 male patients were tested by PCR. Additionally, shell-vial culture method was performed for the urethral/endocervical swab samples which were collected from 46 female and 14 male patients. Four (2 females, 2 males) of the patients (7.1%) showed positive results by both of the methods. In five (8.9%) of the urine samples, internal control tests were found negative, indicating the presence of amplification inhibitors, and the culture results of these patients were also negative. Since the PCR method (Cobas Amplicor CT, Roche Diagnostic Systems, NJ, USA) which was used in the study included internal control programme to identify inhibitors in urine, the sensitivity was improved. As a result, the perfect (100%) correlation between culture and PCR methods, lead to the conclusion that PCR is a rapid and reliable method for the detection of C. trachomatis in urine samples, however more detailed studies are necessary related to the sensitivity and specificity of PCR method.
Ciçek
Candan
C
Ege Universitesi Tip Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Izmir.
Ozacar
Tijen
T
Kolday
Kahraman
K
Tinar
Sivekar
S
Tuzcuoğlu
Yücel
Y
Bilgiç
Altinay
A
D000293:Adolescent; D000328:Adult; D001437:Bacteriuria / Q000382:microbiology; D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000652:urine*; D005260:Female; D005831:Genital Diseases, Female / Q000175:diagnosis*; D005832:Genital Diseases, Male / Q000175:diagnosis*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000592:standards*; D012680:Sensitivity and Specificity; D014521:Urethra / Q000382:microbiology
D004269:DNA, Bacterial