[Specific features of albumin interactions with hemiacetals of aflatoxins and sterigmatocystin].
Aflatoxin B2a (AB2a), aflatoxin G2a (AG2a), and hemiacetal of sterigmatocystin have been shown to form immunoreactive conjugates with albumin. The conjugates were formed following incubation of solution mixtures at room temperature for 1 h, as demonstrated by spectrophotometry and enzyme immunoassay. Anti-AB2a antibodies reacted with AB2a, aflatoxin B1, and aflatoxin AB2 (100, 8.8, and 5.9%, respectively); a similar result was obtained for anti-AG2a antibodies reacting with AG2a, aflatoxin G1, and aflatoxin AG@2 (100, 2.5, and < 1.0%, respectively). Binding of anti-AB2a and anti-AG2a antibodies to solid-phase conjugates of AB2a or AG2a exhibited similar analytical characteristics.
Kononenko
G P
GP
All-Russian Research Institute of Veterinary Sanitation, Hygiene, and Ecology, Russian Academy of Agricultural Sciences, Moscow, 123022 Russia.
Burkin
A A
AA
Soboleva
N A
NA
D000348:Aflatoxins / Q000737:chemistry* / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity; D007124:Immunoenzyme Techniques; D011485:Protein Binding; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000276:immunology; D013053:Spectrophotometry; D013241:Sterigmatocystin / Q000737:chemistry* / Q000276:immunology
D000348:Aflatoxins; D000906:Antibodies; D013241:Sterigmatocystin; D012710:Serum Albumin, Bovine